LOCUS       DAOSLE010000001        65833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_508_length_65833_cov_8.335963, whole genome shotgun sequence.
ACCESSION   DAOSLE010000001 DAOSLE010000000
VERSION     DAOSLE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 65833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65833
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_508_length_65833_cov_8.335963"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1849)
                     /locus_tag="PK576_00005"
     CDS             complement(236..1849)
                     /locus_tag="PK576_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOE60004.1"
                     /translation="MRSMVAVFLGCLALGAQAFTVSTIYSDNMMLQRGKPIKVTGSGA
                     PNETVEVTFDGETVKAAANAHGRWLATLAPREACKVPKTLTVTGGGKTVTFKNVLVGD
                     VWVCSGQSNMHWNIAWGGCYHQEDVKAEAETNAPYFRGIDLRRGSLLFPAAEVRLDGC
                     SYKKWVKMGKRPGEVSALGFMFGLKLSKACPDVPMAIISLAQGGSRIEPWIPLDGYDG
                     IKGLEEEAAAAELYDFGKPQGRAVLNGVLKDISGWAKGFAALSTNDFPYAPQPMLPRP
                     LKGHAGLYNHMMAPLETFPFKGVIWYQGESNEDDAKYGIKQRALITSWRRRFDTDMPF
                     YFVQLPNFGEKNKPGDFRMRKYSAIRILQAESLDLPLTGMAVTTDTADDNQLHPKNKL
                     DVADRLARMALNDLYGQKQPNEGRSPVFRAAALSADGKEIVIHFKYAEGGLANGTREI
                     EKAPVLTDAKDVKGVVVTLANGREVAPESAVIDGEVFRISVDGDQPIAAVRYNCFAYP
                     EHHVWSKTTGLPLAPFKTASLVTCSAIPK"
     gene            complement(1869..2981)
                     /locus_tag="PK576_00010"
     CDS             complement(1869..2981)
                     /locus_tag="PK576_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60005.1"
                     /translation="MKSRILMVAILAAVSKGMAARTSRVFGDNMVLQSGRPIPVWGTG
                     EVDETVSVAFGGNTASCRVGADGKWQVVLKAESPAVAPRELTITAASGAQTFKNVRVG
                     DVYIIMGDRHVGWPFWSVGAEIKTDLDRYADTNLCYFISPIQRRASYPLDDLEPPTPH
                     AGFWRSVVNKDRLSSFAYFYARERAVKTKMPVGVIVVTGGAYDRCDARMFMRPEAYAA
                     SQNDKGNPQAYDALNPTSEKGLAALKEAAARIQTWADDALALPQGEYPASNMPELPNL
                     EKYGQVTTHYNYQMSPFAKTPIKGAVFYAGYGWGYDPKEAPLAEELKTDMRRVWGDDI
                     AFTIVPVPEKTVMEGDCPSECNKPLEVVRNLDASNK"
     gene            complement(3004..7059)
                     /locus_tag="PK576_00015"
     CDS             complement(3004..7059)
                     /locus_tag="PK576_00015"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOE60006.1"
                     /translation="MMNKIMAALAVAFGVFSALAAETVELKAKPTVTPVELNFGDTVK
                     FTLLNGETRTIRLVDTSAQIIEIPTVEGLVFMFDVTLEVDGHRVKIDRYTATQQAFYE
                     PIVINGMRIWADMALSVYDVIPMRYPGTGNQRQRPWKDMRLALQDATIEDCPVKLSKW
                     FDYRHPSIFIGDCYHGGDCWMGSFGAGVAHGGLDVNQREGDPLWAPMDFDDQGYFQSL
                     ATGSNNNRWRGWRTWPDGSIWAFQCHHVITQYVAQGMSVKQGTHLFDGAGVWVGWYNH
                     NHYEFKVANGKMPDWNAGGRNSADGRGGRALEHGQPEVYHLDPWIYFRQIFRNEREKT
                     GYVYPEMAPPGPAKTAEAVVFELTKGGSDDVYWDFGDGAFGRGAKVTHRYAKPGLYAA
                     RAVIVREDGSLDEVIRHLTVNGAASLLPSLIVQSDDHRFRPWPTGTQYAFGYDLVNYR
                     PGGFEIKAPIGQRNLPTESRTFRFANAGGGVLPPIAAALSEPCDWIVLQPTADGVTFT
                     VDPMKAKDGVNEAAIVLKGEGLLNGEVTVPVRLAKVKCGYYTGSFRLDSNAHTCFYAT
                     PGFWCSHRLSGSKRYYKMSGNRPESDAIARFIPEPMAAGTYRVRVDGDAPWAAGSKCW
                     VDVKDAAGLHRVRFEPAKSWTVGTFRFAAGGGNYVEFRTQDSVGSVAVNHLAFDRLPD
                     TPPVAKAPYVPETIELKAKPTVTPVEMNCGDVVKFTLLNGATRTLTLIGTSARMAACT
                     TTETILGFDATLEIDGHRVTFQRNTCEPEAFYEPFVVNGLRIWPDMALKIFDVMPMRQ
                     ACEGNGRRRPWKDLRLALQDATIEDCPVKLSKWLEYPRLAPSVNDGRVGGMCWMGSFE
                     GHHIHGGLDIRQQPDKTALSAPLDLDDQAPLPERTKHSREGRWRGIRKWDDGSVWAFE
                     CHHIVTQSVPAHVSVKQGTTFLTGGKPHPGHHMHTHFEFRVANNGMPDWDASTYARDG
                     GAGTRALEHGQPAVYHLDPWIYFRQIFRNERERTGYEYPELAPIGPAKTGEAVAFEVL
                     RGCGTATNRAEVCWDFGDGAFGRGEKVTHRYAKPGIYAARAVFARPDGTFDEVVRHLT
                     VDGTRIAVPDLVVKSDEPAFFPHPAGVPQAFAYGRVNLRPGQFAFITDEKRPSAETRT
                     LRFANAGGGVLPKVTATLKTPCDWLTLASVAEGVVLTADGTKAPAGVSEAVIVLAGDN
                     LLNGAVEVPVRFDVGAHTLIKRDRLNLDASGVGFYATPGGWLGLAGGAFTSGGRNRPG
                     EVARFTPQMLEAGEYTVCIPLNTPWPMGHQCNVRVRDAGGVQTVRFDPAKSMTLGAFR
                     FAAGGGNYIEFESAGATGEVVVRKVRLIPNR"
     gene            complement(7062..10904)
                     /locus_tag="PK576_00020"
     CDS             complement(7062..10904)
                     /locus_tag="PK576_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60007.1"
                     /translation="MNVEKVVATVAIAAGVCFAANAAEWKTLRDLPVSAVGNAAYLTS
                     MTERPWWNKAWTRRTPILVSSAAKASCGKIVVDAVVDFGEQVNPAEVRLVTPWETEVP
                     CWVEAKEGTKLELYFETDLRIEENRPFLVYWGNPAAKAPDLWPTMTMRVNDEEVRIAN
                     GVLDVTFDNLHRTSGLLKKIRVLASQTPNELLDRASGFAWDGFSLRSGAAEPWSKAKV
                     VADNPFKKQIRFDSKKVTLDFTLYANQPRVDYAYTLKPGNEQANISISWACGDGTGED
                     DLFYPGLAGKVLSLRASLDHATDCIPNPHYEGLGKYLAEGWYAIRDRKQHEVVGMIFD
                     RPTLASFDYSGAGQYAGERSVMTFRHQTEKDRPVGGSGALVALMGGVDDVRNEYLRYC
                     KAPIISVGKTEAYREIAVRRPRIDRDFCADYNIGKGAGAGWESGEPLEGGEWCSNIVD
                     RVRSLGGNIVRIGGYGWQDLAITKELYDRIAKVWALPTSRSRNRKMPEWQEGQYTGEK
                     FRQHCAAAHAKGVAVSLWSGFVPGVGFDNDMLWDKDAQELDTELQCLYADLGADIIYN
                     AMACREGAHIPTDVIKGRGTSWWLWDDPRDFFKTEDIRTEAIKKFYRDCKRRHPDTPV
                     VVMNSECSIIERETMMTDQVGAFDSVYCEMLPLCDNPRTLPHTKMAVKRLRALYDNEA
                     GRTVHAHFYYYGHDCVQRILQVELPYVWGINGFTFENLTYENFGKEQPNITADAYRFL
                     EYTKLGDKVARMAPVKDLAVFRDNRAYVDDVINHRRGKPYAYSTQQDGRANAIAEIRN
                     CVYDIVIDPFFTAKALAKYRAVYVPEDLSFGEDRAKELIGYVQAGGGAIVEGRTVEGN
                     GLSKLGLTDGKVTDLGKGKIVWLKDVLTDRLARRDAKAFAQAKELMASVGCLAPFTLP
                     SKTLDANLQISEEGMFLGVFNDGKEEDKGRATLNLSHLPNFSHLSNPLYVLDVKRGVR
                     FRYTNGFEVVVGPEQCGYYLIGDEKFTALPETSGGAWFGASASHVGEPGRGCKMPVLD
                     RSEAFPRVHALEYVRGSEKKPTMIRRSMLAAFDIKQVTPDAYDPSLFAAAIRKATYLH
                     LCANAKGADRAFADCPEELKAMLKRGGAILFDRTRPGPNAQKFLKEIGVFDPYPSAKT
                     KIGDGKSVWDETFPSNHLLSAFNRVKEGYFGNDFIRRLGRDGGRYNLAFGQWDAETQL
                     TPFRAALNKDYTHLIIQEHVLGAGKVAFNENARSFNDWYESKMYGDALLSWLIGMPVK
                     DYKDKAERYNGGPGKAVE"
     gene            complement(10971..12563)
                     /locus_tag="PK576_00025"
     CDS             complement(10971..12563)
                     /locus_tag="PK576_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE60008.1"
                     /translation="MAMNVKPGAVFVPCLLAAGVLLAAEPFCEEAKGWKRVPAVSDEF
                     DGKALDKSKWDDWCASFPGRVAAGMRGEPCVNGFRFAPDNVSVEDGELVLTARLLTDE
                     ERKSHHQEHLRHGPYATSIVKSRKKQVYGYFEIRAKTMKACVSNAFWLYDPHSDDPRV
                     KYTAGDVSEEIDIFEVTGRPDSRGRNPDCSRKYYNTFHLYCTPYLEGVINAKKINPPD
                     RAFVTKVDFDFCDDYHVYGLLWTPEKLVWYLDGKPVKERKNDGLFHRPLHVTLDSEVF
                     VSWFGVPDPKDLPAKFRIDYVRVWECPVSGNVALRPRMKIEKDSYDWFERHNEAMACA
                     RARKPEIVFVGDSITHFWAGGRTLGGEAAHPRWKQKFGAYRTLNLGYGWDRTGNVLWR
                     LDHGEMDGLDPKLVVLHIGGNNFSATKNYPGNNAEETAEGVLAVLDKIRTKVPQARIV
                     TMAIFPFGEKPDAPHRVKARAANKILAVEVPKRPNTVFLDITDKQVGADGIYPKSRAN
                     DFVHPTDAGYDIWYEALKPYLP"
     gene            complement(12600..14105)
                     /locus_tag="PK576_00030"
     CDS             complement(12600..14105)
                     /locus_tag="PK576_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60009.1"
                     /translation="MTISKNGLLAIVVGSMLSGVAATGDSGRVEVTLDRSPDRVETPL
                     NPPLVRIGTLVAREKPDPSDDRWQIGCEVLDRDFANFDEYKGFLVPLGIRNVRFQGGW
                     AKCERTKGVYDFSWLDAQVDWVLAHGLNPLIETSYGNPIYNDVRADLGGGFPSGEKAL
                     AAWDAWVNALTRHFKGRVRDWGMWNEPDNNPQHTPERVAAFNVRTARIVKRNIPDARV
                     AGLVFNHPDTNRFDRALKAMGPDTALFWRFVYHSYEYNPESSYGDVAHCQALVKKYAP
                     HAVLWQGENGAPSETVYTLALLGRPWSEYSQAKYNLRRMLGDLGRSIPSSVFTICDYN
                     HVGRELGTFGLLRANKSHEVIGVKRAFYAVQNVATLFDSRTKLVARRNYSKDGTIEIW
                     EYRRDGKRLYVFWDHKDGKSVPPSESFELRPFVMDVPKGAEALETPVWIDLLTGRIYE
                     FPKERVLRHSDGITYTDIPLYDSPCVLTERLVVDAAMLAPTQSNVRTRASH"
     gene            complement(14128..16614)
                     /locus_tag="PK576_00035"
     CDS             complement(14128..16614)
                     /locus_tag="PK576_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60010.1"
                     /translation="MMGIRMKSGIVLMGAVFFTFSAAFGGVWEKYAGNPVLGNAKLGT
                     CFDVNVVTNGPAPYTMYFSWRPKKAIALVRSDDALSWTQEPEICLEADPTSGWEDDLN
                     RSCTVFKDGKWHMWYTGQAKGGSKIGYATSADGVKFVRVRKDPVMVPENDFEKKSVMN
                     PYVRWDAQRGVWRMWYAAGETYEPNVLCYAESKDGVDWKKWEKNPMFGKGAAESWDRD
                     RVGGCETHPLPDGRWAMFYIGYSDIDTARIGCAISPDGVSDWKRIAQNPIIAPDLCTW
                     DASACYKPSVLRDEKNNRWMLWYNGRNGWSEYVGMALHEGLDLEAPSSKVPDTKALLS
                     TYVRGFNKFDNECVTNAIPNSDAERFLSENVPLFACPDKDIERTYYFRWWTFRKHLRH
                     GTGVWTVSEFLPKVGWSGKDNTIVCPAGHHFLEGRWLRDPKYMTDLARFWLTDKECTH
                     RWQYSSWLYTGVCKFAAVAGKEALADELLDAAVAYFERWEKGFDRRKWDEEGKGSIRM
                     GGDGKGRFLSARTNEGTEFTLTPSGYRPLFNAAMWSEAHAISEVAARLGRSDLATRYA
                     EKAAVIRRGLEEICWSPEIAFFVAADTNRTRCAVRELHGYAPWYFGVPTDKKADWSQL
                     SDPQGFAAKYGLTFPERRAKGFVLAYSGHECQWNGPSWPFATSIALTALINDLHARPS
                     IPNPQRSTFNFCLWQYAAQQKLIKGIAVPWIDENANPDKPDWISRTILHKRSKEKVKF
                     ERGKDYNHSTFCDLVISGLVGIIPEGAKGFTVNPLAPAAWDYFMLERLNYRGHEVSVV
                     WRRGHGGLTVTVDGKVAARRDDLGRLAIRF"
     gene            complement(16659..18545)
                     /locus_tag="PK576_00040"
     CDS             complement(16659..18545)
                     /locus_tag="PK576_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5696 domain-containing protein"
                     /protein_id="HOE60011.1"
                     /translation="MNKNDLTNAAHRICNADTFGTGHKTIRLAVFAVSVLTAAAPCVQ
                     AAEVTLDYTYIDGRTRREKMPLDSENRLVVKADHIGPGVKTLDVAPDFATAKKGEDGY
                     FLMPDGLYGTFRADNGKFYPAARNYHPLFIMKTPRVNFMAHATGLAYSYETHVVAKNG
                     GYRLFNRYNFNGDPPYEDLVIRFRFGAADVSWCAFAREYRDDQLKRGACRPINERMKD
                     FPALAYAATNIEVRLRLGWKPVPAKVRDQTPFTEPPVKVAITFDRCRDIMREFKRQGI
                     PEAEFCLVGWNKGGHDGAYPQLFPVEPKMGGETKLRALLKDAKTMGFQTVAHNNYSDA
                     YLIADCFDEEFTLKKKDGLPKTAGTWGGGFQYLTCPQRMCERFLVKDLEMMRDLGFFG
                     LHYTDVIASQPLRKCYDPRHAINEADCIKWYARIQSETKKRLGGFASEGIFDYCVGDL
                     DYCLYAYFYKLVPGELNKMVDRHVPLAQIIYNGIVLINPFCGTVNYTIKDNVKRLKLV
                     EFGGRPIFYFYTNFVEGFRWMGDEDFTCATDAELTASVAKIKQGYDEFCRLSDLQFVF
                     MDEHEYLASNVTCTTYANGTKVIVNHGTADFPYKGEVVPAGDWRRFDSTEGNPVASLL
                     RSDR"
     gene            complement(18630..20228)
                     /locus_tag="PK576_00045"
     CDS             complement(18630..20228)
                     /locus_tag="PK576_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOE60012.1"
                     /translation="MKKTWIIGCSMAAFAAMANVKPANVFSSNMVLQRDKVVPVWGTA
                     DAGEEVKVSFAGQSVAAKADADGRWEVKLQPMPASKENRTMVIEGKNKIEFKDILVGD
                     VWLCSGQSNMEMSFNWGIYDGEAFKKEAAEKFDPIRCVKVSKIHSTWPKADVKLDHPW
                     ARTTANGGIANWTATGYFFARRLVQELDIPIGILDDNWSGCRIEPFIPLSSFKNYSAT
                     NGAYAKQVKSFLELQEKTDPRTEAGRKVFAKYAAEMKAFLAAVEAAVAEGKLPEADMP
                     ALPSVSATSQYNAMIAPIVRFPIAGAIWYQGCSNGQEGESYTEKTEMLVKGWREAWGY
                     EFPFYWVQLASFTAKTDDPKGGNGYALIRDAQRLALKRLEKTGMAVTIDVGNDKDIHP
                     KAKVFVGERLAAWALKGAYGRDVVPSGPLFRKAEGLGTAEVTLSFDYVGGGLTAGEKD
                     NLSNAPVKLLPDAKLVGFAAEVEGGQFVWADAKIAGDKVVVTAGGKTIRNVRYGYRAN
                     ATGLANLYNREALPASPFATENKW"
     gene            complement(20274..21932)
                     /locus_tag="PK576_00050"
     CDS             complement(20274..21932)
                     /locus_tag="PK576_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60013.1"
                     /translation="MKTLTIVAAVCAPAVLLAGLSVNPVFMDNMILQRDKPIPVWGRG
                     EAGKEVTVDFAGARARATVDADGRWAATLPALGVSNDDRTMTVTMGEKQGFFARLFGC
                     APEGERVTFANVLVGDVWLCSGQSNMEYSFCWWPYSMDLSKAFCTNTPNFRYLRVARH
                     KAPVPRETPEIQGKWSVLTGQVWFTASCVGCFFAQTVMEKTGKKIPIGLIDSSWGGMN
                     IEPYIDPTCLRDDDPKALKAIKDRYDENANPATAKGRMKWDAYLARQKAFVADIEAAV
                     VDGRPPAGDPAEVPVSHTYWNGCYQAMIHPFAKYPVKGALWYQGCGNYPDNENYQYLL
                     EGLVRGWREAWGYTFPFYWVQLSSFDNKGVVDDAPAGGLGFTRVREGMRRAMATIPHG
                     GMCVTFDLGDPKDIHPREKLHVGQRLAQWALKNEYGFDDIVPSGPNYRAMKIEGDRIR
                     LSFDYVGSGLAVGYKNNFDDRDVKLMPEAKLSGFAIAGEDRTWFFADAVIDGDTVVVS
                     SKDVKKPVAVRYAYRTSPIGANLYNRELLPASPFCTDDWEREKK"
     gene            complement(22034..23515)
                     /locus_tag="PK576_00055"
     CDS             complement(22034..23515)
                     /locus_tag="PK576_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60014.1"
                     /translation="MKMCIAVMLGLAVTVTFADGTKAYARPGSDGGPLYTARGDAPRI
                     AKMITEAQAAGRREVVIPYRNDAANDDKWIIEETVLIPSGMKVILDGARLVQADGVCC
                     NLFANEHAWTDDRCTAKAEDRDIAIEGRNGATLDGGNWNGLGERSKPPELAGKRLVHN
                     CFVYFHNVKGFKVSGLKCRHQRYWATCFSFCEQGVIRDLRFEADISWVSEDGRDHRPN
                     RLPGKYVNLWVKNGDGVNLRKGCHDIVVENITGWTEDDVVALTNLCGEEMKDIVEGKS
                     TDIHHITVRNIRAGTWLWFNQVRLLCCDGAKVHDVVIDGVHDEYDPSWMNWQRNASAV
                     QINDSAEEYYRKRNCAMGEMRDIVIRNVTSGAGFPIRLFCPMQNVTIENLHLLPGAKG
                     ALAVQCDAEFDNVTVSGITADETVRLFSFLNFHQAEGAITVRNARVPEAEHLIRNSGT
                     CQVTLENVEIGKLNGEREIKSCGGHWIGDKVPLYQDLKPGTRP"
     gene            complement(23531..25009)
                     /locus_tag="PK576_00060"
     CDS             complement(23531..25009)
                     /locus_tag="PK576_00060"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE60015.1"
                     /translation="MKRQMMLMTGICLAGAAWAQEKAPLPPAVQAQMAAEFAKAKLVT
                     EGLSDYERPWTLSAYVPNDRIVWEVGERPEAVFEVRGADGRLLTKEQLTNVTMSVSWY
                     RTHETTVEDIVLSQEGNPVRRILTLDKPGVVAVTAKLRGIPEPLNGRYPNVRDLRQAR
                     VDEKGKRHSLTLSYRVGALFNPLEIKPGKPIPEGFDAKWREFLEEDAKYSNVPKHFRR
                     RGTNDAENVWVYAGVLDSPGGEVHFDWGVPKVVREGKGKLPIRVFLQAYGTGFHFAET
                     WISGELTLGINCHSISNRASNAYWDEARKRHGNFGFPTNENAKVETCYHINMLRRDIR
                     ALRWAMSHPGWDGTNLYVHGDSQGGFQTFALSGLFPQITKAWSMCPWYVDVGGSQKHW
                     RPLWQPGPDFADPVHHITRTKAQNITYTFGICDSAGPADGMMAMFNGIPDTTAFAEIT
                     IIQNRGHDAGHTSKDLFPHYVFTKRFGKVTGAYHLPTGETKR"
     gene            complement(25174..27273)
                     /locus_tag="PK576_00065"
     CDS             complement(25174..27273)
                     /locus_tag="PK576_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HOE60016.1"
                     /translation="MNVEKVLTLSVLAAAVCALSGFAADGAPTAKIKPCAGSEIRQFA
                     PGERLFFDRHFVISKKVALQLVGKSFVYASINGISFRVTQSGEILALTPPAEFTDKGG
                     KPSQESELKKLGFAEVNDIPVSQLFNTSDKEKVRVWRKQVKEGERYELDYWCVIAGFD
                     PKPAVTVKKDVPRETLYNGIVLETDPRDRTDMRKYGRYPLPVPYLDNPPAVIPVDAGR
                     QLFVDDFLIASTTMVRTWHKAQKDPKNPVLRPETPLERGALGPIKTPMAAPFSGGVWF
                     DATDRLYKCWYCAGWFDGTAYAYSKDGYNWERPVLKVDGKTNRIVKPETRDGKNCHRD
                     SAAVILDPDATDGNRFKAFIWSRPQGGEIFTSANGTDWEWRAACAWSGDRSTLFYNPF
                     RKVWCYSLRDFWSARSRQLAESKDFVAGVAYTNRVCWQRADCCDRRDMRWVYCEEGNY
                     EKEPPEPSLYNLDCVAYESLMLGVYAIMSGADNSEFQRRGSPKLTELHLGFSRDGYHF
                     SRPEDRSPFIPASRTYGAWDRAYLHSNAAICLVKGDELLFYYTGFAGQIGEWKDHPVG
                     GLYTLAQMGIARLRRDGFASMDAGRCGGELVTRPIRFTRGDRLFVNANAAGGELKVEI
                     LDEDGQALAASKPFTANAVKSEILGGLSSYAGKTIRLRFTARDTQLYAFWFSDASGRS
                     RGYLAGGSPEADGLKDE"
     gene            complement(27366..29291)
                     /locus_tag="PK576_00070"
     CDS             complement(27366..29291)
                     /locus_tag="PK576_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60017.1"
                     /translation="MKTKAFRATVIVAATILGAAVQAAEWYVDKDSTADTHDGGQATP
                     FLTIQEGLDAASANDIVHVAKGSYLISAPLTFKADGVSLVGDDGVAANTVIDAQQLTN
                     CVTSTYFNSHFHNLTFTNGRCEVTSAYGGGGLCLTKGGAVISNCIVTSCAVIGTGEIG
                     IAGGGIKANGGDLIDSLVEHCCISNTVTEAYTNATSCNGGGVQGLSTGRRSIIRNCSA
                     VCTRGVARYNFMQGGGAHGGSWYDCAFISNRLVNPLEIYNCRGGGLFTALTTVSNCYF
                     EGNYITGKGSGAFLDRVYACGNNSFIGNIGSAQAGAAMHGIAAFDCYFEGNDLPGGGN
                     ECGSGGLYLDQNGVAHGCAFRNNSGILGSAMQSHGGTVVSNCLFDSNLCTYAGKYGGG
                     VVYYGNQRLSTFLSDCVFINNSSTSDGAAFYAFGTSNATVRSCLFVSNTVKHVLYILE
                     WNVAQSGIAAGEGACGRVENCTIADNTLLGSSYALKGSQYDLFQRNNLIYNNCLEDTT
                     TSRALEDWYAGTNVVRHCFIDSRASNAASFDKDGNVIGSDPMFRDSAQGDYRLTRRSA
                     CYNAGTNLAWMTEGALDMGNGFRETHPATITAFGETFPVGVKLEKIDPCARVEGGRVD
                     IGCFELFRPLVFTMSVK"
     gene            complement(29308..31389)
                     /locus_tag="PK576_00075"
     CDS             complement(29308..31389)
                     /locus_tag="PK576_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60018.1"
                     /translation="MRNVGLVVVLSVCALAVRARTVYVDLNTSAVMPDGMTWETAYPT
                     IGEAVSNLLTNNYESGSAIYIAPGHYALSEPIPAIPGSNPVTFYGVTNVLLKANPGFA
                     QQVVIDGQNATNAFEAIQGYAKGSQFHGLTIVNCRKDVFAATGLELYGGSAINARNSD
                     AVISNCIIRNCWSGGFREGGTAGCIYGGAVAVANGSVVDTLFEDNGITNACASAESQT
                     LTGLRFRGGAICGGGYKEFRGNIFRRNSIVDPTGKWGDLDGGAMHGVGTLTADCRFET
                     NRVWSSRTDKAAGRGGAVFTEGSGKVFSNLVFVGNSSVSQPVCVNASAPALFTHCTFV
                     GNRTERYGASLTTSLKLGAGNEAVIRHCLFKDNYSPGGVADGSGTAVHAHLNDTQGGY
                     RGLFIHDCAFVGNVNKDFGMAALSTWSGNGGAYVSLSNCYFEANLSATNSGTHALLAI
                     NSGLNFSLVDCSFIGNQSGNNGNQPLVRQSGRENEVGSTTNSVVRNCFFYDNASSRVL
                     DISNPVADIGQEFPGTRVESCTIAGNRLSGTSEAILAQTGKKFYVCNTAVWGNYKAGT
                     ADNYNLNASMAAQTSHSYIGPQAIAVDAFTTNVLSGNITEIDPGFADPAKGDFRLKGR
                     SPLKNAGKAQPWMAGANDLSNQAVVWDETLTQGGCDVVRVPALPRIFPTVPDIGCFES
                     WAPLGTGMYVK"
     gene            31594..32277
                     /locus_tag="PK576_00080"
                     /pseudo
     CDS             31594..32277
                     /locus_tag="PK576_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
     gene            complement(34854..36740)
                     /locus_tag="PK576_00085"
     CDS             complement(34854..36740)
                     /locus_tag="PK576_00085"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60019.1"
                     /translation="MGRLTVAAVSVWVAGVCISAQCSSALYGADAGSSPRGGGGCDAW
                     YYPQMSMELYRLEELKRNAQAAGMYVGYPGSVPPYAAAVEFRRRFKPGETREYFTHGR
                     AFSENFDEKTGMLRVSVSARYLDTPALRSPLKGWEARVSGPQRDDTWRAAARYSGGEV
                     PPHLRWLEEDHDYRDLKKIGDYYDTQRELLAYVQTSGEKPPKLFVGESVPEMRNTVPV
                     DSEQATDMVLVANDTWRSRSALALRYFRFEGKAEKVKVIPLWWPGMEEKGTFSAKDKR
                     HEKMREVGVHTLKLCCHDFLIDGIKRDRLPWGGDLTVSLMANAYVLGDPEISRRSLSV
                     LDAYTTDVNGIVTYSMWLIIAHDLHQLYFADREFLEARWWRIKQRIEDLISRTDENGF
                     VARGLDWVFIDWAGPRSATALGMIWTGALDAAAKLAGRINDPRAAEYRALCDKVKANL
                     NRLAWDEGRKLYRVDPFEKNSGFARQANIYAVIFGVAQNERAQAIGDELAKDDLPAVG
                     TPYVFGWELVALARTGHHKEFFTGMETVFGAMLDAGATSFWEGFNAKQKGDERYRFYG
                     RPWAKSLCHAWSAWPAFIFVSEAMGVKPTSDGWKTHEVKPIPGAEGMKADIPTPSGRT
                     KIGL"
     gene            37045..38946
                     /locus_tag="PK576_00090"
     CDS             37045..38946
                     /locus_tag="PK576_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOE60020.1"
                     /translation="MRLDAIVRLLLTVSFVAHVRCFAGPVVTPFMERTRPGAVRPEGW
                     LLDMARKARDGYTGHMDAVDVQFSRSWRNETRPRGKDLLWVSDPGAWSCEGGAYWFDG
                     LVKLAYQLDDPSLKALASNRLDTVLSEMNPHAIGFCWWLDRNDPQQVLEMKISDAWLI
                     WVTGMFARPVCAWHEVSGDARALKALDFALDEHAFSYGKFPTLPSGAYAGYQVTRSER
                     IARALDEFWKKALHQPGQLPAVFSQYLTPPPAHLEETLNIRRLHERLLKIPTRHGVIA
                     SEALLSIFDGYLWTGNTNLLNAVRGWYAFFDRHMRQPYGVTTMDEEWGHPGPERGTET
                     CVVAAEGWTRINLLAALGEGCWGDDVERNFFNAGRNCMTEDYRRHVYLQQPNRLTASD
                     VTRCSFTGARGDRLARYDTKHWPLCCTAALNRILPNYVASMWMLPADGGVAATLYGPC
                     RFAAELSSGSFEVEEHTDYPYSDTITFKIVKSPNAAFPFHVRFPGWCANPELQVNGRQ
                     TVIAAQNGFAAITRIWRAGDVVTLRLPMKPTVEKMFNYNEPRGREYASVSVGPILYAL
                     PISSKDDNNPEGDVHVPLVSAGFDAASVRVGDAMRMLTIPDSKGNSLKLVPYGDAKLR
                     ISMFPVDGD"
     gene            39175..41163
                     /locus_tag="PK576_00095"
     CDS             39175..41163
                     /locus_tag="PK576_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60021.1"
                     /translation="MKETNVIRVLPALGCGLLAFAASAVEINVASVTDLRAAIADTVN
                     VPDGSTIVLQASPTHYDVSADGLGPIRLTRSLTLRGSTENPRDTVLTGNGMTNILYVG
                     GTDTLACNLTVTNGLSYLVDSRKWYRGGGIHVNGSSAVVSNCVVTGCHFDTRTDYLTG
                     TAVAEMTAFGGAGVYANGSSLVLDTLIENCWMFNDRKQKVTGAGAGLFIGGKAWGTDT
                     PSRAYRCEVRNCSTETMSVGGGTGALVGGGLYVSDAGIVDNCRVHHNWVRPTDDSSRC
                     YGGGLYVGTGGIVSNSLVYLNTCQKCVTNTSGNASGGGVAIEGAGLFCTSTVSNNYSY
                     GDAGGVFHINYPSGGLRDSLIIDNEAVGSGGAGTGGPAATGCVFHRNTSGGGGGGWLT
                     GGSAGWFSNCVFEANATGKWGEGKAARVQRGGSTPFVFESCWFVNHTVTDGKAVLQFD
                     GSPLPTYRAHIRNCVFVGNTGSCPFYSSYTATGPEIDNCTFVASDTEYYAIGAGSKRE
                     TNVVVRNTLFHGYKNGGLPPAFATDKPEHVSNCYDQVATTLPKDGDGNILNGNLAGTI
                     VGEPGFRNLAETNLTLRGASPLRGKGLVLDWMKTQDGSADWTACDAGAGTYMIEKVAD
                     YGVAVRFGRRYPRYTGGADAPDIGAFEHYGTADVMYIK"
     gene            41192..45022
                     /locus_tag="PK576_00100"
     CDS             41192..45022
                     /locus_tag="PK576_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60022.1"
                     /translation="MKRSLAMGLALAAVLPCVEAGMKTILDVPVAGVTNTAYLTGMAS
                     RPWWNAAWKRRAPILVSSHADVPEPKAVVDCVVDFGEKVNPDEVRVVTPWETVVPCVC
                     EKGKAEGQGQEPAAVRLLFQTPLRIHENKPFLVYWGNPAAKRETPVTALMMSETANEV
                     RILNGVLDVTIDKKHVTGGFLRKLRIMGTDASNELLERATQWAWNGLEMTLGGATDWS
                     DGVVTEDNAFVKRVRFEGTNGAVTLSVYADQPRVDWAYALKKGDRCELNVAWAVGGGV
                     AYDKFVYPGATGRLLTFDAALDSPTDSIPQPRFEMAPWFGEGWYAIASKRSPSVCGLL
                     FDRPALQGLWYNGSGQAFGEWTRLTFAHKARKDAPVTGSGSLVALPGQALDVRREYLR
                     RKTPLTVSVGAAQPYAEYPYVRPRLDRDFIADINCGKDSGHGWRSGKPLPDPAWASNI
                     VDHLRSYGVTGIRVGGYFWWDMPLTEKLYRDLERQANRPLVAPDCRKRFTGWDPDQYR
                     GTDFLEMANAAHAKGLVVKQCGDFIPGMYGREFDTKFDADIQRTALDLHLRMAEVGGV
                     DVIYNNFEGQEQVILPQDVKREKHNDYWNWKDPDEFFAWQDKARVLVKAFYDAAKKAR
                     PDLPVLVYNSENGELSREIFMSEQIGSFDTVVVEMLSGLDFSRCKHVAKRMRALFDNE
                     AGRTVHHHYYFMKPDAMHRIKEIELPFICGVNGFSHENQSYENFSREQSEIAADFYRF
                     AEYTRLGEKAARMAPVRYLGVFRDSVCYREDIRKGRHGQPKDRPYFNRAEQDGRCRAL
                     GEIRNLDFDFVMNPFFTAKALAKYRIVYVPEDDVFSDALAKELVAYVKAGGTAILEAK
                     TAGNTVIKSLGLKDGVITPLGKGKILWYEKVRTDGLAKRDEKTIREVAAAIEALAGPM
                     PHRIDGASSLDGVLQSGSDGMLLGVHNAGDKADAGRVTIAKALRSGPSLYVLDVKTGV
                     RFPYTNGFDIAIGPRQCGFYLIGDETFTAVPETKTAVWNGPAVAAARPNGIPAQVNDD
                     PSFVPAKAVEFTRGDGKGNPTAIRRSREAMIDVTPVTPADCYPPVLARALSTATYIHF
                     SSLGTLSADEKKTVDDVFVSCADELKALLKRGGTILFDRSPAGPQTRAFLKEIGVFDP
                     YAHPAPKLGDENTVWTSETNHPFAGNLAVKKGGFGRFQATERSFAKWDAARQVAPVRM
                     VRNHDVAVFVLQENVLGTGRVVFLENRFAFSDWYENRAFGDALLGWIVGRPIEEHAKK
                     SLQRAGGIGVALSE"
     gene            45040..47742
                     /locus_tag="PK576_00105"
     CDS             45040..47742
                     /locus_tag="PK576_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60023.1"
                     /translation="MKHMLLLTACALTGARLLPAFGDVTPAALPGGYTELPYVESTYN
                     GGQWVYTDYVPEWNHRIVCKFRVNQVGTWNSPGVFCARGTATNDKGYCLSFGQGGNNR
                     VNLPGALQCWTGGWAAPSSAPFFYPDLLTDYEVDVNVELKKWCVDDGERWNTREHTSF
                     TAGSPLAIFCRHELGNSLSPSSTFGANNCMAGRIYYLRAYDANGTLIHEFVPARDDNA
                     MVPTKYFGFYDTVDAKFWPNCGATAFATDPSVPSNVRVVDRADYDTDEEAGTALVATV
                     AAAFPTDRIKVTGGTKAAPHVYRITSPLLMEMTNTRLFGDSDDAEATVIDAGGTSAGV
                     VVKGSGISVSGLTVRNATNYVNGTTYCGGGLALIAEGSVASNMIVCGCVNGGVEKALR
                     GGGVYLYKSTLVDALVKDCVISNDMDTTGKTAFYGGGVYAERSTVSRLEVSGCLAVNN
                     QMCESMWWATGAFRGGGLMLNESSATDCYIHNNMITNGPKQTSSCNVSHGAGVALMGA
                     STLSRSLVTGNSAPCSAAGVNLESASAVIDCTISSNTVFFAWSGAYPGGGLYLRNASQ
                     RAVNCLLEGNRNLGTYWDAGGGAGVIEAGVFLGGVVRGNGSYNTHGGGFFLNGACLVS
                     NVVFDANWSTDSSDRCGSGAIEFRGGGAVVRDCWFVRNIGGDNANNSYGTVFYGSYDT
                     GSVLQNCFFANNTGACVLGGSIETNKADCVAEACTFVKNHVSRGAVWCTALDYSVPGT
                     VQKMAFTNCVFWKNGENEDKPGIPDKFKNVSTKVSYCYDEIGTGLTPGDYHNLTAANG
                     EPFYADAENGEWRIGRKASVLVDNGLKLDWMGDGAKDGPLDMGDGTWSEAPAATISVR
                     GKTMDVGVIVTRNNAQPRISPQNGLPDIGCAEYQAVNGFILRVH"
     gene            47811..51713
                     /locus_tag="PK576_00110"
     CDS             47811..51713
                     /locus_tag="PK576_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60024.1"
                     /translation="MRSFNFSHAVLALSTILIGASASALDILDVPVAAVSNRVYLADM
                     PERPWWNRAWKRRAAILVSNMSDVREERAMIDFIFDVSEAVEAKSVRVVTPWGTAVPC
                     VAEPVRQGEGALITARSRDVPALVAPTALRLLFETSLRERENRPFFIYWGNPDAPTQG
                     IDSPMTLSCREDEVRVNNGIIEVVFDNLRRTDGLMKKLKVNASQAPSELLWRTTGFAW
                     RGFEFSAGRAPKPWSKAVITADNAFRKTVTFTNEDAVLDFTFYAGQPRVDYVYRLKDG
                     LHNARIDVSWACGGDCGHDDFTYRGLSGQLLTFRAALDHVTDCMPQPEYYRFPWLSEG
                     WYAITDRRNRDVVGMVFDRRALGGVSYIGQGQSSGEYVSLNFNHAPKGKTPVTGSGAL
                     VATVGEARTVATLYAWLTHPPKVFVAKAESYREIPVKVPVLDRDFCVNVNVGGWRSST
                     PLPGDEWAPNIIAHIRALGANTILLGQLTDYSWTSLPISKDLFDRLCAHKKARNPSWK
                     APEWKGGYDGRLLKRICAEAHSNGLAVCTWHDALPGFSYGGKFDAEEQALCREMQALY
                     PMCGVDSTFNALGGGEGVEFPDDDLKKFGRAYWKWPDPEPFFASRRLSGEYMKAFYEY
                     SHKVNPGTKVMVFNSENSELERDMCMGYYPGVMDTLFCEFVAGMNLSKIKHTAKRLRG
                     YFDNRDGHTIHAHYYNMTKDYAHRIGQLEMPFICGINGFSQESMTYENFDRDLFEITA
                     DFNRFALYTKLGPKVAKMAPVKNLAVLRDMACFEEDCRKYRTMPRDWWHASRHDARVN
                     AFAEILSYNYDVVINPHFKAGSLARYKAVYLTDDEVLPEALAAELVAYVKAGGGAVIE
                     GSTGERLNVEEVKGWEDGKVIELGKGRIVWTKEVLTDPMMKGDAKAIARVKALVASVG
                     GVAPFEIASKSLDGILQAGEEGMFLGVYNKGKTEDRGKVTLYVGSPSYVLDVKKGVRF
                     PFTNGFEIAVGPRQCGFYLIGGDAFTAIPAAQEAAWMGAALASVRPAGQKPPENPDPA
                     FRPYVAVEFTSVRGEGAKAKAAGITRSRLAGIERRRFVTDEATLCRTLKVDDLDVWRS
                     NNTDVKTYSAQAFAAALKTAHYVHFSANPKDCDAAFADCAEELKALLRRGGGLLFDRN
                     GTGPAARRFLAEIGVFDPYPTAKRGIGDGAPAWDETFPTNHPYYATNETKPRYGVQRL
                     EADGGQYSLAFTKWDPQTQLAPFRTARDPSAAELVIQDKVLGAGKVVFNQNSRSFNDW
                     YETKTYGNVILSWFIGMPVDEHAKKARFFVGGIGESVK"
     gene            51728..55606
                     /locus_tag="PK576_00115"
     CDS             51728..55606
                     /locus_tag="PK576_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60025.1"
                     /translation="MMKGRVKQLVIAAVLAAGSVEAKTALDVPVSGVTNKMYLSSAAT
                     RPWWNTDWARRAPLLVSSMADIQSGAGWVDCVVDFGETVNPDEIRLVTMWETEIPCVA
                     EKVDGGRVRLIFQLPLRIRENKPLLVYWGNPKAEKPTFIETMNVTMDGATARLANGRV
                     ELTIDRRQEAGNTIKGFRLVGSQTGDLVTLRATGAPWKGFTFATGRSNVVWKTCRVTA
                     DNPFKKQVTLDAPEATLVWTMYPGQPRADYAYTLKRDPSARIVVAWCPGGGAAWDDFY
                     YTGLTGARLTFRGSLDEVTDSGGFPHNDHLEKWMKEGWYAICDRKNGDTAGMVYDRAS
                     LARVSYGAAHYNGGERVSLTFEHASKKGESLRGAGSGALVGTFGGADQVAAEYARLVA
                     PPQVFLGKAESCRTIPIRLPDHTKDFMGEFVTGMNPGNWEHLRPGTNPDWADNAANYL
                     RRFGATAIRLTGYGWQELCLTEDLYNRCKTIIREDPKIADHVKNRPIADWSVERYAGV
                     NLRKVSEAAHRKGLGVSTWTGRGAEIFGGLEADVTETPLYRQLDFDLQNRYPKSGAGV
                     VYNSQCSLEGPWMPKALRQERGNEYWRWEKPEDFFNAQDDFIRYMKAFYAQSKRENPK
                     TPVFVFSAENGGLNRELPLADLIGAFDAEYCELVPGYLASAQGIKHSVRRLRSHFDNN
                     PHTAFHHFYYYTSDHGWRISQLEVPFAFGVNGFSHEVMMHDTIDVELNEITGDFYRFA
                     EYTRLGGKVSKMAPVKHLAVLRDARLFRENVLNKRPNEQESRLRGFAALRNYPCDIVA
                     NPFFTCDSLKRYTIVYVPDNRVFTDEQAAELLAYVEAGGNAIVEGVTGEKVKGLVDGE
                     ITVLGKGKIVWSKEVLSEQLAKDDASKVLPLLRKLGGEEPYSVKAGGSCDGLLQASGE
                     GFFYAAYNVAKHPSKGRFVMKEEVRKSLFVLNVRTGGRKPFDGSFEFEVGAQQCGFWL
                     IGDEAFTAVPEAKACVYDGASAVSAKPAPVKLPQHDVEKAKAFKELEIVEVLSLNPGF
                     LGYQTGGEIGRIDRHERLKLKLTRFYPDEKAVAEVVKPEELDTWKQINKGLYNPFDET
                     AFAKALKTAKVLYLRGSAAQDLPFVRCPQAVKDFLAGGGGIIFDCLDANTNVTAFLKS
                     VGVEDPMAAGRFVKSCGQRAVKAPGLEHPIVRDTNERINDFNREGFCRYHLTGGGWKG
                     WSKDQFAPVRAFEDPEQIATVIVQENVCGKGRVVFSRNWNAWWSYRSSMCDGLLSTNI
                     LSWLFDYDVNAYESDLQVKNGGPGEVVGEQ"
     gene            complement(56085..59936)
                     /locus_tag="PK576_00120"
     CDS             complement(56085..59936)
                     /locus_tag="PK576_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60026.1"
                     /translation="MKPKSILLAVLISVCSVPYSVFAQGWNALLDWPVSTVTNKAYLT
                     SMTERPWWNDAWQKRVPLLVSGQADEKDDKVIVDAIVDLGEKVEPKEVRVVTPWETVV
                     PCVCEKAEGSKVRLLFKTTLRQHENKPFLVYFGNPAAQAEKISADVTLETDEHTFRIR
                     NGVLDVVFDRRHVTEGLIRSLRILGSNCRTVFFDRATGYAKAGFVFHPNATNSWDKGT
                     VTADNALVKSIRFECADSTLTFTLYAEQPRIDWSYEIHRGDEASIRMNWGLGTGSAFD
                     DFFYCGKNGKILTQRAGLDHVTDSMTNPEGRFEDWLGEGWYAIGERRMGDIAGLAFDV
                     TAAGRLGYDSYYGVSAGLKLRHKLERGEKAAGSGAVVATLGDVNDYRRICKRIAKPVI
                     VSVGAVQAKVAKPYRIPRLDRDFCFDHNVGYGYGGDSGTAEPLMKDPAWADRICERLR
                     SYGSTAVCLMGYPWWMMPIEDKALYDRLIAAQTSGAFEDWVPKRKSPSWEEMKGRGQE
                     ILAFTKAIHAKGMGVHTWCGFVPGWSSKANGHFMKEINDLSVDMMSLRVKCGQDCGYS
                     NAIEGEGVALPSDLVKKNGGSPNYWSWENPQEAFDKFDFQHGLMRDFYTKFKKLHPDI
                     PVFLWNSENGEYSREKFMSEDEGYFDTMVVEMLPHAGFPHTKHVSKRMRSHFNNRVGH
                     TVWHHYYIMNPKTTERIYQIEWPFVCGVNGFSQENLSYETKDLEMSEVTADFFRFAEY
                     TCLGDKVARMAPVKNVGVYRDPKMFRADVLKKRLDKPYGAGSQQDGRVRSFSEIKNFA
                     YDVIGPKFFTAKDLAQYRVVYVPEDEVLAEGEAKELLAYAEQGGNAVFEGELSDGAKK
                     VFGELEDAKVKTLGKGKVLWFKDVRTDRLAKRDAKAIAEMRKTIADLGGVEPFTLTGS
                     WMIDGILQAGPDGMFLGAYNDGRAEEKGIVTLSPTVAHVRTTPLYVLDVKTGIRRSVT
                     NGVFEIAVAPRQCGYYLIGDDAFTAVPVVKVAEWKGAGVGSFRPRPHVPPVIDITGFK
                     PIVAIEFVRANQKGKPIRIARSVEAKYDVRAFTPASYDVKDCKAALAEARFIHFIDTD
                     TKAMDLVFADCAEELKALLKRSGTILISRSETGPAAQKFFKEIEVFDPNPSAKAGVGD
                     TWATWCGPEDHPYLTSCHKSLGKEEEVWGTKFWLWRSLCNYFQYRRVFGAWDAAKQQV
                     LFRPKVDGEKYAGLVIQEKVLGTGRVIFNENQRCFTDWYESKQFGDNWLSYITGVPST
                     EHAIKATAYHGGLGEIVEPKW"
     gene            complement(59962..60825)
                     /locus_tag="PK576_00125"
     CDS             complement(59962..60825)
                     /locus_tag="PK576_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60027.1"
                     /translation="MKGKRNVLAAVALAAAFAFADDGYLVVGHGGLMEKGGDSPIAKE
                     LVARFSPKNGTAVAVDVVEVTNAVALKACAPKSYAGIILVPGFDTGKMKDHSIVNAEH
                     LAAMNALLDSVRAKAVVAVEEGGDFENYQRLRDAFAGRAAVITTRDGGARLAMSWKRF
                     APEGMRRIALALKHAADPAKRDGIHPLPGKVTMEESWMVLDFETPALQGQSLIVSDTL
                     FRKFYVRSAGKTNWTRIDNLYVEDNKVYLDCSKISNPGGVRLNWWHLSPFKNIDHLPT
                     PGFVLALDAGR"
     gene            complement(60854..61792)
                     /locus_tag="PK576_00130"
     CDS             complement(60854..61792)
                     /locus_tag="PK576_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE60028.1"
                     /translation="MTAAKNMNRRAFLASAGALVAYPIWGKVPEDEKPLVRVGLVTDI
                     HHAYLPEAWFDNGRFYDRALPRLEEAIKVFNERRSDLVIELGDFKDIAKKKDPKPGED
                     DRDKETVIKLTKEAEAVFAQFNGPRYHVFGNHDNDMLDKSEFTALCPNPGIPPEKTYY
                     AFDQNGVTFIILDGNFNLKGEDYTGSKINWDWRESLIPPTQIAWLKETLAAAKGHVVV
                     CIHQRLDSGARPLHRVANGAEVQKILADSQKVAAVFQGHDHIGGYMKERGVGYYTLRA
                     IVTGPQRENNAYAEAAVYPSGAVVVTGFAQAVTVHI"
     gene            complement(61833..63818)
                     /locus_tag="PK576_00135"
     CDS             complement(61833..63818)
                     /locus_tag="PK576_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60029.1"
                     /translation="MKTSRLITAGWLVFACVSAWAQVPKLYVKTAADGGNDVTGDGSW
                     DAPYATIQRAMDVAVKEQKNVLTTISVQPGVYPGSNYVNSTSYFVDVRAQKRVNGVDV
                     DNDNPRETVIDGSINRQFGFDLRGWKMSDDGTLYRQSVCGFTVTNGLWTYYWRGYELT
                     TSHMGGGGVTVYNGAMASNMIVCCCITEPDDRIPPGKTQGNQHGAVQINGNNSLFTHS
                     FVYNCTNHNTAASLTGEITGGGVYMYGGTIRDCTVSNCWLIADKDVNGNRRGGGIYLA
                     ENQNGLVDRCVVVDCGTSGTRSCEGGGICNMKPNVVTNSFIANCTANGNGGGICCSYG
                     GNVYDCTVSNCINTAGYGGNFGATSGSEQWLGTFRGCRFINCSGTSVYLGFGIMDGCL
                     IENNTGTPLQMTGKNTILRNSVIRNNTGKKAAFAHTTGADGTGTVERCWFVDNDCGNN
                     PVALRGQTYARWLILRDTLIAGNTCKRGVSITAKTSWSDVTDPWVRVENCTVAGNTCS
                     DVGIGVNAGSGGTSYPDEITITNCLVTANNGKQAFNDYLKVNGTVGNIAYCAADEGKG
                     THLPDGCHNVTGTITFREGTFIPTGSSVATERGFALPRMREAGASDMGDGTFTIMKSG
                     EWGVKIVPNNVKSRIIGVAPEIGACEYMPSGTTVMVN"
     gene            64167..65645
                     /locus_tag="PK576_00140"
     CDS             64167..65645
                     /locus_tag="PK576_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOE60030.1"
                     /translation="MSTALRYDVIALRILSGRVLGVLLGVFALTETLWADFSPDRAVM
                     GEKYRLNWNAAVDAEIDARIEKHRKADCVVALDGVPDGTVVCVEQLTHDFKFGSHIFN
                     FDQLGRDDWNDMYKSTFTNLWNAATVAFYWKNYEPVEGAVRFADGPHDGAAFWNSKAT
                     LSPKEKRLAFNEWRRPAPDPIVNFCLRHGLSVHGHAMIYGAYTPGWATNIVDASRLDA
                     VYEHRIRTLCKHYGDRIDQWDVVNESVRANSMPESPDDEGKWRFRMPKCYTFKSFKTA
                     EEHLPRRVRLAINDAGVMVKPYVPFIRHLLGKGARIDIIGVQMHIFSRDDVLKVADGL
                     ECMPNLTSWDPVDQLATLTKLSRLGRPIHISEITIPAPDDTPEGWRIQAQLMYDNYRL
                     WFSWPDVCRITYWNLVDYSYDKESLASGLYTKDLRRKPSYEALDRLINREWKTRMSIV
                     VNNGRIAFRGFRGMYLLSWMDAEGTMRSKRINLKGAVERVEF"
BASE COUNT        12445 a        20283 c        19991 g        13114 t
ORIGIN      
        1 aaatcctgtt aatcctgtcc aaactctccc cacctatgac gccgacggca acatgacgtg
       61 cctcggggaa tggcgtcatt catgggacgc ggagaaccgc atgttcgatg gtgtgggagg
      121 gacacggggc gaaattccca tgtccccacc cgttcccttc cgaggatgtc gacggtggta
      181 ctgaagttac cgttgatctg ggaacggcga tgcgcgtcga aggttttgtg gtccttcatt
      241 tcggtatcgc actacaggta acaagggatg ctgtcttgaa cggggcaagc ggcagtccgg
      301 ttgtctttga ccagacgtgg tgctcggggt aggcaaaaca gttgtagcgc acggcggcaa
      361 tcggttggtc gccgtcaacg gaaatgcgga aaacctcacc gtcaatcact gctgactccg
      421 gtgcgacctc gcgtccgttc gcaagcgtga cgacgacgcc cttgacatcc ttcgcatccg
      481 ttagcaccgg cgctttctcg atctcgcgcg ttccattggc gagaccgcct tcggcatatt
      541 tgaagtgaat gacgatctcc ttgccgtcag ccgaaagcgc cgcggcacgg aatacgggag
      601 agcgaccctc gttcggctgt ttctgaccgt agaggtcatt cagcgccatg cgcgcaaggc
      661 ggtcggcgac gtcaagcttg ttcttgggat gtaactggtt gtcgtccgcc gtgtcagtcg
      721 tgacggccat gccggtcagc ggcagatcca gcgattcggc ctggagaata cggattgcgg
      781 agtactttcg catcctgaaa tcaccgggct tgttcttctc gccgaagtta ggaagttgaa
      841 cgaagtaaaa cggcatgtcc gtgtcgaaac gcctgcgcca ggaggtgatg agcgcgcgct
      901 gcttgatgcc gtatttcgca tcgtcctcgt tggactcacc ctgataccag atcacgccct
      961 tgaacgggaa tgtctccagt ggcgccatca tgtggttgta gagtcccgcg tgtcctttga
     1021 gaggacgcgg cagcatcggc tgcggcgcat acgggaagtc gttcgttgaa agcgccgcaa
     1081 agccctttgc ccaaccggag atgtccttga gtaccccatt cagcaccgcg cgtccctgcg
     1141 gcttgccgaa gtcgtaaagt tccgccgcag cggcctcttc ctcaaggcct ttgattccat
     1201 catagccgtc tagcgggatc cacggttcga tgcgactgcc gccttgagcg agcgagatga
     1261 tcgccatcgg cacgtcagga caagccttcg agagcttaag cccgaacatg aagccgagcg
     1321 ccgagacctc gccaggacgc ttgcccattt tcacccactt cttgtaactg caaccgtcga
     1381 ggcgtacctc agccgcagga aagaggagtg atccgcgcct caggtcgatc ccgcggaaat
     1441 acggtgcgtt cgtctcggcc tcggccttga catcctcctg atggtagcat ccgccccagg
     1501 cgatgttcca gtgcatgttc gactgccctg agcagaccca cacgtcaccg acgagaacgt
     1561 tcttgaaagt gacagtcttg ccgccgcccg tgacggtcaa cgtcttcggc accttacacg
     1621 cctcacgcgg cgcgagtgtc gcgagccatc gtccgtgcgc gttcgccgcc gccttcaccg
     1681 tctcgccgtc aaacgtgacc tccacggtct cgttcggcgc gcccgacccc gtcaccttga
     1741 tcggcttgcc gcgctggagc atcatgttgt ccgaatagat cgttgacacc gtaaacgcct
     1801 gcgcaccgag cgcgaggcac cccaaaaaca ccgcaaccat tgacctcatc gtcttcctcg
     1861 atctcctgtc atttgttcga cgcgtccaga ttgcggacga cttccagcgg cttgttgcat
     1921 tctgagggac aatccccttc catcacggtc ttctccggta ccggaacgat ggtgaacgcg
     1981 atgtcgtcgc cccagacacg gcgcatgtcg gttttcagtt cttcggcgag cggcgcttct
     2041 ttcgggtcgt atccccatcc gtagcccgca tagaacaccg cgcccttgat cggcgtcttg
     2101 gcgaatggcg acatctggta gttgtaatgc gtagtgactt gtccatactt ctccagattc
     2161 ggcaactcag gcatgttgga agcgggatat tcaccctgtg gaagcgcgag cgcgtcatcg
     2221 gcccacgttt gaatgcgggc ggccgcctcc ttgagcgcgg cgagcccctt ctcggatgtc
     2281 gggttgagcg cgtcatatgc ctgcggattg cccttgtcgt tctgactggc cgcataggcc
     2341 tcggggcgca tgaacatacg cgcatcacaa cgatcgtacg ccccgccggt cacaacgatg
     2401 acgccaacgg gcatcttcgt cttcacggcg cgttcgcgcg catagaagta ggcgaaagag
     2461 ctaagccgat ccttgttgac gacactgcgc cagaagccgg catgcggcgt cggcggttcg
     2521 aggtcatcga gcggatacga cgcgcgccgc tggatgggcg aaatgaagta gcagaggttt
     2581 gtgtcggcgt agcgatcgag atccgtcttg atctccgcgc cgaccgacca gaacggccag
     2641 ccaacatgtc ggtcgcccat aatgatatag acatcgccga cgcgcacatt tttgaacgtc
     2701 tgcgcacccg atgccgcggt gatcgtcagt tcgcgcggtg ccaccgcagg gctctcggcc
     2761 ttgaggacga cctgccattt gccatccgca ccgacccggc atgatgcggt gttgccgccg
     2821 aacgcgacgg atacggtctc gtcgacctcg cccgtgcccc acacgggtat cggacgaccg
     2881 ctttgaagga ccatgttatc gccgaagaca cgcgatgtcc tagccgccat tcccttactg
     2941 accgcagcca atatcgccac catcagtatt ctgcttttca tattccttgt cctgttctcc
     3001 cctttaacga ttgggaatca gacgcacctt gcgcacgacg acttcacccg tggccccggc
     3061 actttcgaac tcgatgtagt tgccgccgcc tgcggcgaaa cggaaggccc cgagcgtcat
     3121 tgacttcgcc ggatcgaacc gcaccgtctg cacacccccg gcatcccgta cgcgaacgtt
     3181 gcattgatgg cccattggcc agggcgtgtt gagcggtatg cacaccgtgt actcgcccgc
     3241 ctccagcatt tgcggtgtga agcgcgcgac ttcacccgga cgattgcgtc cgccggacgt
     3301 gaatgcaccg cccgcaagac caagccatcc ccccggcgtc gcatagaagc cgacaccgga
     3361 cgcatcgagg ttgaggcggt cacgtttgat taaggtgtgc gccccgacat cgaaacgcac
     3421 cggaacctca accgctccgt tcagcaggtt gtcgccggcg aggacgatca ccgcctccga
     3481 aacccctgcg ggcgctttcg tgccgtccgc cgtcaggacg acaccctccg ccaccgacgc
     3541 caacgtcagc cagtcgcacg gcgttttcaa cgtcgccgtc accttcggca gcacgccacc
     3601 gcccgcgttc gcaaaacgca atgtgcgcgt ctcggcactg ggccgcttct cgtccgttat
     3661 gaaagcgaac tgaccagggc gcagattgac gcgtccgtat gcaaacgcct gtggaactcc
     3721 cgccggatgc gggaagaaag cgggttcgtc ggacttcaca acgagatcgg gaaccgcaat
     3781 ccttgtcccg tccaccgtca ggtggcggac gacctcgtcg aacgtgccgt caggacgtgc
     3841 aaacaccgcc cgcgccgcgt aaatccccgg tttcgcatag cggtgcgtca ccttttctcc
     3901 gcggccgaag gctccgtcac cgaaatccca gcagacctcc gcgcgattcg tcgcggtgcc
     3961 acatcccctc aacacttcaa acgccaccgc ctcgccggtc ttcgcgggac cgatcggggc
     4021 caactcggga tattcatagc ccgtccgctc gcgttcgttg cggaagatct ggcggaaata
     4081 aatccacgga tcaaggtgat acacagcggg ctggccgtgt tcgagcgcgc gcgtccccgc
     4141 tccgccgtca cgcgcatagg tgctcgcgtc ccagtcgggc attccgttgt tggcgacacg
     4201 gaactcgaaa tgcgtgtgca tatggtggcc gggatgcggc ttgccgcctg tcaggaacgt
     4261 cgttccctgc ttgacgctca catgcgcggg gacggactgc gtcacgatgt gatggcactc
     4321 gaacgcccag accgatccat cgtcccattt ccggatgcca cgccagcggc cctcgcgcga
     4381 atgtttcgtc ctttccggca acggtgcctg atcgtccaga tcaagcggtg ccgaaagcgc
     4441 cgtcttgtcc ggctgctgac ggatgtcgag accgccatgg atgtgatgcc cctcgaagga
     4501 acccatccag cacatgccgc cgacacgccc atcgttgaca ctcggcgcca gacgcggata
     4561 ctcgagccac ttcgagagtt tgaccggaca gtcttcgatc gtcgcatcct gcagggcaag
     4621 gcgcagatcc ttccacggac gccgccggcc gttgccttcg catgcctgcc gcatcggcat
     4681 gacgtcgaaa atcttaagcg ccatatcggg ccagatacgc agtccgttca cgacgaacgg
     4741 ctcatagaac gcctcgggct cgcatgtgtt gcgttggaat gtcacgcggt gtccgtcgat
     4801 ctccagcgtc gcatcgaagc ccaggatcgt ctccgtggtc gtacatgcgg ccatgcgcgc
     4861 cgaggtgcca atgagcgtga gtgtgcgtgt cgcaccattg aggagcgtga atttcactac
     4921 atcgccgcag ttcatttcaa cgggcgtcac cgtcggtttc gccttcagct cgatcgtttc
     4981 aggaacgtac ggcgccttgg cgaccggcgg ggtgtcgggc aggcgatcaa acgcgagatg
     5041 gttcacggcg acgctgccga cgctgtcttg cgtgcggaac tcgacgtaat tgccgccgcc
     5101 cgccgcaaac cggaaagtgc cgaccgtcca ggacttcgcg ggctcgaagc ggacgcgatg
     5161 aaggccggcc gcatccttga catccaccca acatttgctg ccggccgccc agggcgcatc
     5221 accgtccacg cgtacacgat aggtgcccgc tgccatcggt tcggggatga aacgcgcgat
     5281 ggcgtccgat tcgggacggt tgccggacat cttgtaatac cgcttgcttc cgctcaatcg
     5341 gtgcgaacac caaaaaccgg gcgtagcgta aaagcacgta tgcgcattgc tatcgagacg
     5401 gaaacttccg gtgtagtaac cgcacttcac tttagcgagc cgtaccggca cggtgacctc
     5461 accgttcagg agtccttcac ccttgagcac gatcgccgcc tcgttgacgc cgtccttcgc
     5521 cttcatcgga tcgaccgtga acgtgacgcc gtccgcagtc ggctggagga cgatccagtc
     5581 acacggctcg gaaagtgccg cggcgatagg gggcaacacg ccgccgccag cgttcgcgaa
     5641 gcggaacgtg cgcgattccg tcggaagatt gcgttgtccg atcggtgcct tgatttcgaa
     5701 accgcccggg cggtagttga cgagatcgta accgaaagcg tattgcgttc ccgtcggcca
     5761 ggggcggaag cgatggtcgt ccgactggac gatcagcgac ggcagcagac ttgccgcacc
     5821 gttcacagtc agatggcgga tcacctcatc gagtgagccg tcttcacgca cgatgactgc
     5881 gcgtgccgca tagaggccgg gcttcgcata tcggtgcgtc actttcgcgc cgcgtccgaa
     5941 cgcgccgtcg ccaaagtccc aatagacatc atccgatccg cccttcgtca gttcgaaaac
     6001 gaccgcctcg gccgtcttcg ctggaccggg cggcgccatc tcaggataga cgtagcccgt
     6061 cttttcgcgt tcgttacgga agatctggcg gaagtagatc cacggatcaa gatggtagac
     6121 ctcgggctgc ccgtgttcga gcgcacggcc gccgcgtcca tccgcgctat tccttccgcc
     6181 cgcattccaa tccggcatct tgccgttggc gaccttgaac tcgtagtggt tgtggttata
     6241 ccagccgacc cagacgccgg caccgtcgaa aagatgcgtc ccctgcttga cgctcatacc
     6301 ctgcgcgacg tattgcgtga tgacatggtg acactggaac gcccagatcg aaccgtccgg
     6361 ccaggtgcgc cagccgcgcc aacggttgtt gttgctgcct gtggcgagcg actggaagta
     6421 gccctgatcg tcgaagtcca tcggtgccca gagcgggtcg ccctcgcgct ggttcacgtc
     6481 gaggccgcca tgcgcaacgc cagcaccgaa ggatcccatc cagcaatcgc cgccgtgata
     6541 gcagtcgccg atgaagatcg aggggtgccg gtagtcgaac cacttcgaga gtttgaccgg
     6601 acaatcctcg attgtcgcat cctgcagggc aaggcgcata tccttccacg gacgctgccg
     6661 ctggttgccg gtgccgggat aacgcatcgg gatcacatcg tagacactga gcgccatatc
     6721 cgcccagata cgcatgccgt tgatgacaat cggctcgtag aacgcctgtt gcgtcgcagt
     6781 atagcggtcg atcttgacgc ggtggccatc gacctcgagc gtcacgtcga acatgaaaac
     6841 gagcccttcc acagtcggga tctcgatgat ctgcgcggag gtgtcgacaa gccggatcgt
     6901 ccgcgtctct ccgttcaaga gcgtgaactt cacggtgtca ccgaagttga gttcaaccgg
     6961 cgtcaccgtc ggcttcgcct tgagttcgac cgtctccgcc gcgagggcgg agaaaacccc
     7021 aaaggcgacc gcaagggccg ccatgatttt gttcatcatt ttcactccac cgctttcccg
     7081 ggtccgccat tgtagcgttc ggccttgtcc ttatagtcct tgaccggcat cccgatgagc
     7141 cacgacagga gcgcgtcgcc atacatcttc gactcgtacc agtcgttgaa cgagcgcgcg
     7201 ttctcgttga aggcaacctt gcctgcgccg agcacgtgct cctgaatgat aaggtgcgtg
     7261 taatccttgt tgagcgcggc gcggaatggc gtgagttgtg tctccgcatc ccactggccg
     7321 aacgccagat tgtaacggcc gccgtccctg ccgagccgac ggatgaaatc gttgccgaaa
     7381 taaccctcct taacacggtt gaaggccgag aggaggtggt tggacggaaa ggtctcgtcc
     7441 catacggatt tcccgtcgcc gatcttggtc ttggcggacg gataggggtc gaaaacgccg
     7501 atctctttca ggaacttctg cgcgttcggt cctggacgcg tacgatcgaa aaggatcgct
     7561 ccgccgcgct tgagcatcgc cttcagttcc tccgggcagt ctgcgaacgc ccggtccgcg
     7621 cctttggcat tggcgcagag gtgcaggtac gtcgccttgc ggatggctgc ggcgaacagc
     7681 gagggatcgt aggcatcggg tgtgacctgc ttgatgtcga acgccgccag catcgatctg
     7741 cggatcatcg tcggcttctt ctccgaaccg cgcacatact ccagcgcatg gacgcgcggg
     7801 aacgcttcgg aacggtcgag caccggcatc ttgcagcccc tcccaggttc gccgacatgt
     7861 gaggcggaag cgccaaacca tgctccgccg gacgtctccg gcagcgctgt gaacttctca
     7921 tcgccgatga ggtagtagcc gcactgctcc ggtcccacca caacctcgaa gccattcgta
     7981 tagcggaaac gcacgccgcg cttcacgtcg agaacgtaaa gcgggtttga gaggtgcgag
     8041 aagtttggaa ggtgtgagag gtttagggtc gccctcccct tgtcttcctc cttgccatcg
     8101 ttgaagacgc cgaggaacat gccttcttcg gaaatctgaa ggttggcatc gagcgtcttg
     8161 gacggaagcg tgaacggcgc gaggcagccg acagacgcca tcagctcctt tgcctgcgcg
     8221 aacgccttcg cgtcgcgacg cgcaaggcgg tcggtgagaa cgtctttgag ccagacgatt
     8281 ttgcccttgc cgagatccgt caccttcccg tccgtaagcc cgagcttcga cagaccgtta
     8341 ccctcgacgg tccggccctc gacaatggca ccgccgccgg cctgcacgta cccgatcagc
     8401 tccttcgcgc ggtcttcgcc aaaggaaagg tcttcgggca cgtagacggc gcggtatttc
     8461 gcgagcgcct tggcggtgaa aaacggatcg atcacgatgt catagacgca gttgcggatc
     8521 tccgcgatgg cgttggcgcg tccgtcctgc tgcgtactgt aggcatacgg cttgccgcgg
     8581 cggtggttga tgacgtcgtc aacgtaagcg cggttgtcgc ggaagacggc gagatccttc
     8641 acgggcgcca tccttgcgac cttgtcgccg agcttggtgt attctaggaa gcggtaggcg
     8701 tccgccgtga tattcggctg ctccttaccg aagttctcgt aggtgaggtt ctcgaaggtg
     8761 aagccgttga tgccccagac gtagggaagc tcgacctgca ggatgcgttg cacgcaatcg
     8821 tgtccgtagt agtagaaatg agcgtggacg gtccgtcccg cctcgttgtc gtaaagcgcc
     8881 cgcaggcgtt tcaccgccat cttcgtatgc ggcagagtgc gcggattgtc acagagcggg
     8941 agcatctcac aatagacgga gtcgaacgcg ccgacctgat ccgtcatcat ggtctcgcgc
     9001 tcgatgatcg agcattcgga gttcatgacg acgaccggcg tgtccggatg acgccgcttg
     9061 caatcgcggt agaacttttt gatagcctcg gtgcggatat cctcggtctt aaagaagtcg
     9121 cgcggatcgt cccagagcca ccacgaggta ccgcgtccct ttatgacgtc cgttggaata
     9181 tgcgcgccct cgcggcacgc catggcgttg tatatgatgt ccgccccgag gtcggcgtaa
     9241 aggcactgaa gctcggtatc gagctcctgc gcatccttgt cccagagcat gtcattgtcg
     9301 aagccgacgc cggggacgaa gccggaccac aggctgaccg ccacgccctt cgcatgcgcg
     9361 gccgcgcaat gttggcggaa cttctcaccg gtgtactgac cctcctgcca ctcgggcatc
     9421 ttccgattcc tggagcgcga ggtcgggagg gcccacacct tcgcgatccg gtcgtaaagt
     9481 tccttcgtga tcgccagatc ctgccacccg tagccgccga tgcgcacgat gttgccgccg
     9541 agcgaacgga cgcggtcgac gatgttcgaa caccactcgc caccttcgag cggctcgccg
     9601 gactcccatc ccgcgcctgc gcccttgccg atgttatagt ccgcacagaa gtcgcggtca
     9661 atccgcggac gccgcaccgc gatttcgcgg tatgcctcgg ttttgccgac ggagatgatc
     9721 ggcgctttac agtagcgcag gtactcattc cgcacatcgt ccaccccgcc catcagcgcg
     9781 acgagcgcac ccgaaccgcc gacgggcctg tccttttcgg tttggtggcg gaaggtcatg
     9841 accgagcgct cgcccgcgta ctgtcccgcg ccgctgtaat cgaaggatgc gagagtgggg
     9901 cggtcgaaga tcatgccaac gacctcatgc tgcttgcggt cacggatcgc gtaccagccc
     9961 tccgccagat acttccccag cccctcgtag tgggggttgg gaatgcagtc cgtcgcatgg
    10021 tcgagcgaag cgcggagcga cagcaccttg cccgcgaggc cgggatagaa aagatcgtcc
    10081 tcccccgtcc cgtcgccaca ggcccatgag atgctgatgt tcgcctgttc attgcctggc
    10141 ttgagcgtgt aggcgtagtc cacacgcggt tgattggcgt aaagcgtgaa gtccagcgta
    10201 accttcttgc tgtcgaagcg gatctgcttc ttgaagggat tgtccgcaac gaccttggcc
    10261 ttcgaccacg gttcggcggc gccggaacgg agcgaaaaac cgtcccaagc aaaaccggag
    10321 gcgcggtcga ggagttcatt cggcgtctgg gacgccagaa cgcggatttt cttcagaaga
    10381 ccgctggtgc ggtgaaggtt atcgaaggtg acgtcaagga cgccgttcgc gatgcgcacc
    10441 tcttcgtcat tcacccgcat cgtcatcgtc ggccacaagt ccggcgcctt tgccgcggga
    10501 ttgccccagt agacgaggaa ggggcggttc tcctcgatgc gcaggtcggt ctcgaagtag
    10561 agttcgagtt tcgtcccttc cttcgcctcg acccaacacg gaacctcggt ttcccagggc
    10621 gtgaccaaac gcacctcggc ggggttcacc tgttccccga agtcgacgac agcgtccacc
    10681 acgatcttgc cgcacgacgc ttttgccgcg gacgacacca gaatcggtgt ccgccgcgtc
    10741 cacgccttgt tccaccacgg ccgctccgtc atcgacgtca gatacgccgc attccctacc
    10801 gccgaaaccg gcaggtcccg aagcgtcttc cattctgcgg cattcgccgc aaagcacaca
    10861 cccgcggcaa tcgccacggt cgccacgacc ttttcgacat tcatcgaata atgttaccat
    10921 tctccatccc ggtgcacaat cctcgccggg gtaaaaaatg gcggcaccgt ttacggcaaa
    10981 tagggtttga gcgcctcgta ccagatgtcg tagcccgcgt ccgtaggatg aacgaagtcg
    11041 ttggcgcgag acttgggata gatcccgtct gcaccgacct gcttgtccgt gatgtcgagg
    11101 aagaccgtgt tcggacgctt cggcacttcc acggcaagaa tcttgttggc cgcgcgtgct
    11161 ttgacacgat gcggagcgtc gggcttttcg ccgaacggga agatggccat cgtgacgatg
    11221 cgcgcttgcg gcaccttcgt gcgaatttta tccagcaccg cgagaacgcc ttcggccgtc
    11281 tcctcggcgt tgttgccggg atagttcttc gtcgcggaga agttgttgcc gccgatgtgc
    11341 aggacaacga gcttgggatc cagtccgtcc atctcaccat ggtcgagacg ccacaggacg
    11401 ttgcccgtac ggtcccagcc gtagccgagg ttcagcgtac ggtacgcgcc gaacttctgc
    11461 ttccagcgcg gatgcgccgc ctcgccgccg agagtgcgtc cacctgccca gaagtgggtg
    11521 atggagtcgc cgacgaacac gatctcgggc ttgcgtgcgc gggcacaggc catcgcctcg
    11581 ttgtgtcgct cgaaccagtc ataggaatcc ttttcgatct tcatgcgtgg gcggagcgcg
    11641 acgttgccgg agacgggaca ttcccagacg cgcacgtagt cgatgcggaa cttcgcaggc
    11701 agatcctttg gatcgggcac accgaaccaa ctgacgaaca cctcgctgtc gagtgtgaca
    11761 tgcaacggac ggtggaagag tccgtcgttc ttccgctcct tcaccggctt gccgtccaga
    11821 taccacacga gtttctccgg cgtccagagg agtccgtaca catggtagtc gtcgcagaaa
    11881 tcgaagtcca ccttcgtcac gaaggcacgg tccggcggat taatcttctt cgcgttgatg
    11941 acaccttcaa ggtatggcgt acagtagaga tggaaggtgt tgtagtactt gcgcgagcag
    12001 tccggatttc gtccgcggct gtcgggacgc cccgtcactt cgaagatatc gatctcttca
    12061 gaaacatcgc ccgccgtgta ctttacccgc ggatcgtccg agtgggggtc atagagccag
    12121 aacgcattgg agacgcacgc cttcattgtt ttcgcgcgga tctcaaagta gccgtagacc
    12181 tgctttttgc ggctcttgac gatcgaggtg gcgtaggggc catgccgcag gtgctcctga
    12241 tgatgagact tccgctcctc atcggtgagg agccgcgcag tgaggacgag ttcgccgtcc
    12301 tctacagaga cgttgtcggg cgcgaacctg aagccgttaa cgcaaggctc gccgcgcatc
    12361 ccggccgcca cgcgtccggg aaacgacgcg caccaatcgt cccacttgga cttgtccagc
    12421 gccttgccgt caaactcatc gctcacggcg ggcacccgtt tccacccctt cgcctcctcg
    12481 caaaacggtt ccgccgccaa caagacgccc gccgccaaca ggcacggaac aaaaacggca
    12541 cccggtttaa cgttcattgc catactccca atacgttgtt ttgaatacct tgccagagat
    12601 taatgggatg cccgcgtccg cacgtttgac tgcgtcggcg cgagcatcgc cgcatcgacc
    12661 acgagccgtt ccgtcagcac acacggcgaa tcgtaaagcg ggatgtccgt ataggtgatg
    12721 ccgtccgaat gccgcagcac acgttctttc gggaattcat agatgcgccc tgtaagcaag
    12781 tcaatccaga cgggggtctc gagcgcttcg gcgcctttcg ggacgtccat cacgaatgga
    12841 cgcagctcga acgactcgga cggcggcacg cttttcccgt ccttatggtc ccagaaaacg
    12901 taaagccgct tcccgtcgcg ccggtattcc cagatttcaa tcgttccgtc cttggaataa
    12961 ttgcggcggg cgacaagctt cgtacgcgag tcgaaaagcg tcgcgacgtt ctgcacggca
    13021 tagaacgcgc gcttgacgcc gatgacctca tggctcttgt tggcccgcag cagtccgaat
    13081 gtcccgagtt cgcgccccac gtgattgtag tcgcagatgg tgaagacgct tgacgggatg
    13141 ctccgtccaa ggtcgccaag catacgccgc aggttgtatt tggcctgcga atactcgctc
    13201 cacggccggc caagcagcgc gagcgtatac accgtctcgc tcggcgcgcc gttttcgccc
    13261 tgccagagca cggcatgggg cgcatacttt ttcaccagag cctgacagtg cgcgacatcg
    13321 ccatacgacg actcgggatt atactcgtag ctgtggtaga cgaatcgcca gaagagtgcc
    13381 gtgtccggcc ccatcgcctt gagggcacgg tcgaagcggt tcgtgtccgg atggttgaag
    13441 acaagccccg cgacacgcgc gtcgggaatg ttgcgcttca cgatcctcgc cgtccggaca
    13501 ttgaacgcgg cgaccctttc gggcgtgtgc tgcggattgt tgtccggctc gttccacatc
    13561 ccccaatcac gcacacggcc cttgaaatgc cgcgtcagcg cgttgaccca agcgtcccac
    13621 gccgcaagag ccttctcgcc ggacgggaat cccccgccga gatccgcccg cacgtcgttg
    13681 taaatcggat tgccgtagct ggtctcgatg agcgggttga gtccatgcgc cagaacccag
    13741 tcaacttgtg cgtcgagcca ggagaagtcg tagacgccct tcgtcctctc gcacttcgcc
    13801 cagccgccct ggaagcgtac gttgcggatg ccgagcggca cgaggaatcc cttgtactcg
    13861 tcgaaattcg cgaagtcgcg gtcaagcacc tcgcatccga tctgccagcg gtcgtccgac
    13921 gggtcgggtt tttcgcgcgc gaccagcgtg ccgatgcgca caagcggcgg attgagcggc
    13981 gtctcgacgc gatccggcga gcggtcgagc gtcacctcga cgcggccaga atcgcctgtc
    14041 gcggcaacgc cagacaacat cgaaccgacg acaattgcca gcaggccgtt tttcgaaata
    14101 gtcatgactt tcaagaatgt ccttttgtca gaacctgatc gccagacgcc cgagatcgtc
    14161 acgccgcgcg gcgaccttgc cgtccaccgt gacggtcagt ccgccgtggc cgcggcgcca
    14221 gacgaccgaa acctcgtgtc cgcgataatt gaggcgctca agcatgaaat agtcccatgc
    14281 cgctggcgcg agcggattga ccgtaaaacc cttcgcacct tctgggatga tgcctacgag
    14341 ccccgaaatc acgaggtcgc agaacgtcga gtggttgtag tccttgccgc gctcgaactt
    14401 gactttctcc ttcgaacgct tgtgcaggat cgtgcgtgaa atccagtccg gcttgtccgg
    14461 attcgcgttc tcatcgatcc aggggacagc gatacccttg atgagcttct gttgcgcggc
    14521 atattgccaa aggcagaagt tgaacgtgga gcgttgagga ttggggattg agggacgagc
    14581 gtgcaggtcg ttgatgaggg ctgtgagcgc gattgatgtc gcaaagggcc aactcggacc
    14641 gttccactga cactcgtgtc cgctgtaagc gaggacaaaa ccttttgcgc ggcgctcggg
    14701 gaacgtgagg ccgtacttcg ccgcgaatcc ctgcggatcc gagagctgcg accaatcggc
    14761 cttcttgtcc gtcggcacgc cgaaatacca cggcgcatag ccgtggagtt cacggacggc
    14821 gcaacgcgtg cggttggtgt cggccgcgac gaaaaacgcg atctcggggc tccagcagat
    14881 ctcctcgagc ccgcggcgga tgaccgcggc tttttcggcg tagcgcgtgg cgaggtccga
    14941 gcggccgaga cgcgcggcga cctcggaaat cgcatgcgcc tcgctccaca tcgccgcgtt
    15001 gaagagcgga cggtatccgc tcggcgtcag ggtgaattcg gtgccttcgt tcgtgcgggc
    15061 cgaaaggaag cgtcccttgc cgtcgccgcc catgcggatc gagcccttgc cctcctcgtc
    15121 ccacttccgg cggtcaaagc ctttctccca gcgctcgaag tatgcgacag ccgcatccag
    15181 aagttcgtcc gccaacgcct ccttcccggc gactgccgcg aatttgcaga cgcccgtata
    15241 gagccagctc gaatactgcc agcggtgagt gcactccttg tctgttaacc agaagcgcgc
    15301 aaggtccgtc atgtatttcg gatcgcggag ccagcggccc tcgaggaaat ggtggccagc
    15361 gggacagacg atcgtgttgt ccttgccgct ccagccgacc ttcggcagga attccgacac
    15421 cgtccagacg cccgtgccgt gacgcaggtg tttgcggaag gtccaccagc ggaagtagta
    15481 cgtgcgctca atgtccttat cagggcaggc gaagagcggc acgttctcag acaagaagcg
    15541 ctccgcgtcg ctgttcggaa tggcattcgt cacgcattcg ttgtcaaact tgttgaatcc
    15601 gcgcacatag gtcgacagga gcgccttcgt gtcaggcact ttcgaggatg gcgcctcaag
    15661 gtcaagtccc tcgtgcagcg ccatgccgac gtattcggac cagccgttgc ggccgttgta
    15721 ccagagcatc cagcgattgt tcttctcgtc acgcaggacg ctcggcttgt agcacgcgct
    15781 cgcatcccag gtgcagagat ccggggcgat gatcggattt tgcgcgatac gcttccagtc
    15841 gctgacgccg tcaggcgaga tcgcgcaacc gatgcgcgcg gtgtcgatgt cggaatagcc
    15901 gatgtagaac atcgcccagc gcccgtcggg aagcggatgc gtctcgcacc cgccgacgcg
    15961 gtcgcggtcc cacgattccg cagcgccctt gccgaacatc gggtttttct cccacttttt
    16021 ccaatcgacc ccatccttcg attcggcgta gcagaggacg ttcggctcgt aggtctcgcc
    16081 ggcggcgtac cacatgcgcc agaccccgcg ttgcgcgtcc cagcggacgt aggggttcat
    16141 cacagacttt ttttcaaaat cattctcggg aaccatcacc ggatccttgc ggacgcggac
    16201 aaatttcacg ccatccgcgc ttgtcgcata gccgatcttc gaaccgcctt ttgcctggcc
    16261 ggtataccac atgtgccatt tgccgtcttt aaagacggtg cagctacggt tcaaatcgtc
    16321 ctcccagccg ctcgtcgggt cagcctcgag gcagatctcc ggctcctgtg tccacgacag
    16381 cgcgtcatcg ctccgcacga gcgcgatcgc cttctttggt cgccacgaga agtacatcgt
    16441 gtacggggcc ggaccgttcg taaccacgtt aacgtcgaaa caggttccga gcttcgcatt
    16501 gcctaatact ggattcccgg cgtatttttc ccaaaccccg ccgaatgcgg cagaaaaggt
    16561 gaaaaacacc gcacccatta aaacgatccc gcttttcatt cgaatgccca tcattttacc
    16621 tccccgaaac cgtcgcaatc gttcttcgcc agcaccttct aacgatctga ccgcagaagg
    16681 gaagcgacgg ggttcccttc tgttgaatca aaccgacgcc agtcgccagc cggaacgacc
    16741 tcacctttgt aagggaaatc agccgttccg tgattgacga tcaccttcgt cccgttcgcg
    16801 taggtcgtgc aagtcacatt cgacgcgaga tactcgtgtt cgtccatgaa gacgaactgc
    16861 agatcggaaa gacggcagaa ctcgtcatat ccttgcttga tcttggcgac tgacgccgtc
    16921 agttccgcgt cggtcgcaca cgtgaagtcc tcatcaccca tccagcggaa gccttcgacg
    16981 aagttcgtat agaaatagaa aatcggacgt ccgccgaact cgacaagctt gaggcgcttt
    17041 acgttgtcct tgatcgtata gttgaccgtg ccgcagaaag gattgatgag aacgatgccg
    17101 ttgtagatga tctgggcgag cggcacgtgc cggtctacca tcttgttcag ttcgcccggt
    17161 acgagcttgt agaagtaggc gtataggcaa tagtcgaggt cgccgacgca gtagtcgaag
    17221 atgccctctg acgcaaagcc cccgaggcgc ttcttcgttt cggactggat gcgcgcgtac
    17281 cattttatgc agtcggcctc gttgatcgca tgcctcgggt cgtaacattt cctgagcggc
    17341 tgggacgcga tgacgtcggt atagtggagt ccgaagaacc cgagatcacg catcatctcc
    17401 agatccttca cgaggaagcg ctcacacatc cgttgcggac acgtcaggta ctggaagccg
    17461 ccgccccacg ttccagccgt tttgggcaat ccgtccttct tttttagcgt gaactcctcg
    17521 tcgaagcagt ccgcgatcag gtatgcgtcc gaatagttgt tgtgcgcgac ggtctggaag
    17581 cccattgtct tcgcatcctt caacagcgcg cggagcttcg tctcgccgcc catcttcggt
    17641 tcgacaggga agagctgcgg ataggccccg tcgtgtccac ccttgttcca accgacaagg
    17701 caaaactcgg cctcgggaat gccctgccgc ttgaattccc gcataatgtc gcggcaacgg
    17761 tcgaatgtaa tcgccacctt gacgggcggt tcggtgaacg gcgtctggtc acgcactttc
    17821 gcgggcacgg gtttccagcc cagccgcagc cgcacctcga tgttcgtcgc ggcgtacgcg
    17881 agcgcgggga agtctttcat gcgctcgtta atcggtctgc atgcgccacg cttcagttga
    17941 tcatcgcggt attcgcgcgc gaacgcgcac caagacacat ccgccgcgcc gaaacggaag
    18001 cggatgacaa gatcctcata ggggggatcg ccgttgaagt tgtagcggtt gaagagacgg
    18061 tatccgccgt tcttcgcgac aacatgcgtc tcgtacgaat aggccagacc cgtcgcatgc
    18121 gccatgaaat tcacgcgcgg tgtcttcatg atgaagagcg gatggtagtt gcgcgcggca
    18181 ggatagaatt ttccgttgtc cgcacggaac gtaccgtaaa ggccgtccgg catgaggaag
    18241 tagccgtctt cgcccttttt cgccgtcgcg aaatcgggcg cgacatcaag tgtcttcacg
    18301 cccggtccga tatggtctgc cttgacgacg agccggttct cgctatcgag cggcattttc
    18361 tcgcgacggg tacgtccgtc gatatacgtg tagtcaagcg tgacttcagc cgcctgtacg
    18421 cagggtgcgg ccgccgtcag aaccgatacc gcgaaaacgg caagccgaat cgtcttgtga
    18481 ccggtgccga aggtgtcagc gttgcaaatc cggtgtgctg catttgtcag atcgttcttg
    18541 ttcatggctt tgagattgtt tcagtggttg agtatccatg cggccatcgc tctgccgcaa
    18601 tacacagaca ggagtgcgat gcgaacgcct taccatttgt tttcagtggc aaatggcgaa
    18661 gccggcaacg cttcacggtt gtagagattg gcaagacccg tcgcgttggc gcgatagccg
    18721 tagcggacgt tccggatcgt cttgccgccg gccgtcacga cgaccttgtc acccgcgatc
    18781 ttcgcatcgg cccagacgaa ctgtccgccc tcgacctcgg ccgcgaagcc gacgagcttc
    18841 gcgtcgggga ggagcttgac gggcgcgttg gagagattgt ccttctcacc cgccgtgaga
    18901 ccgccgccga cgtagtcgaa tgacagcgtc acctcggccg tgccgagccc ctccgctttc
    18961 ctgaaaagcg gacccgaggg gacgacgtcc cggccatacg cgcctttgag tgcccacgcc
    19021 gcgagacgct cgcccacgaa caccttggcc ttcggatgga tatccttgtc gttgccgacg
    19081 tcaatcgtga ccgccatgcc cgtcttttcc agacgcttga gcgcaaggcg ttgcgcgtca
    19141 cggatgagcg catagccgtt gccgcccttc ggatcgtctg tcttcgccgt gaaagacgcg
    19201 agctgcaccc agtagaacgg gaactcataa ccccacgcct cgcgccagcc cttgacgagc
    19261 atctcggtct tctcggtgta ggactcgccc tcctgcccgt tcgagcaacc ctgataccag
    19321 atcgcgccgg cgatcggaaa acgcacgatc ggcgcaatca tcgcgttgta ctgtgaggtc
    19381 gcgctgacac tcggcagcgc cggcatgtcg gcctcgggga gcttcccctc cgcgacggcc
    19441 gcttcgaccg ccgcgagaaa cgccttcatc tctgcggcgt acttcgcgaa caccttccgg
    19501 cccgcctccg tgcgcggatc ggtcttctcc tgaagctcca gaaagctctt aacctgcttg
    19561 gcgtacgcgc cgttcgttgc gctgtagttc ttgaacgacg agagcgggat gaacggctcg
    19621 atccggcaac cgctccagtt gtcgtcaagg atgccgatgg ggatgtccaa ctcttggaca
    19681 agcctgcgcg cgaaaaaata gccggtcgcc gtccagttgg cgatgccgcc gttcgcggtg
    19741 gtacgggccc aaggatgatc gagcttcaca tcggctttgg gccacgttga gtggatcttc
    19801 gagaccttca cgcagcggat cgggtcgaac ttctcggccg cctccttctt gaaggcctcg
    19861 ccgtcgtaga tgccccagtt gaagctcatc tccatgttcg actgccccga gcagagccac
    19921 acatcgccga cgaggatgtc cttgaactcg atcttgttct tgccctcgat caccatcgtc
    19981 cggttctcct tcgaggccgg catcggctgg agtttcacct cccacctgcc gtcggcatcg
    20041 gccttcgccg caacggactg ccccgcgaac gagaccttga cctcttcgcc agcatcggcc
    20101 gtgccccaca ccgggacgac cttatcgcgc tggagcacca tgttgctcga gaacacattc
    20161 gccggtttca cgttcgccat cgccgcgaac gctgccatcg aacaaccaat aatccatgtc
    20221 ttcttcatcc tgaatctcct cgtttcctta ttcctgtttc ccaattccca ttctcacttc
    20281 ttctccctct cccaatcgtc cgtgcagaac ggcgaagcgg gaagaagctc ccggttgtag
    20341 aggttcgcgc cgatcggcga cgtgcgataa gcgtagcgca ccgcgaccgg cttcttcacg
    20401 tccttcgacg aaacgacaac cgtgtcgccg tcgatgaccg cgtcggcaaa gaaccatgtg
    20461 cggtcctcgc ctgcgatcgc aaatccgctc aacttcgcct cgggcatgag tttcacgtcc
    20521 ctgtcgtcga agttgttctt gtagccgacg gcgaggcccg acccgacgta gtcgaatgag
    20581 agacggattc tgtcaccctc aatcttcatc gcgcggtaat tcggccccga cggcacgatg
    20641 tcgtcaaagc cgtactcgtt cttaagcgcc cactgcgcga gacgctggcc gacgtgcagc
    20701 ttttcccgcg gatggatgtc cttggggtcg ccaaggtcga aggtcacgca catgccgccg
    20761 tgtggaatgg tcgccatcgc gcggcgcatg ccctcgcgga cgcgcgtgaa cccgagtccg
    20821 cccgccggcg cgtcgtcgac gacacccttg ttgtcgaagg acgagagctg cacccagtag
    20881 aacgggaacg tgtaccccca cgcctcgcgc cagccgcgca cgaggccctc caggagatac
    20941 tggtagttct cgttatccgg atagttgccg cacccttggt accagagcgc gcccttgacc
    21001 ggatacttcg cgaacggatg gatcatcgcc tggtagcagc cgttccagta ggtgtgcgag
    21061 acggggactt cggcgggatc gccggccggc ggccggccgt cgacgaccgc cgcctcgatg
    21121 tccgcgacga acgccttctg ccgggcgagg tacgcgtccc atttcatccg gcccttggcg
    21181 gtcgccgggt tcgcgttttc gtcgtagcgg tccttgatcg ccttcagcgc cttcgggtcg
    21241 tcgtcccgga ggcacgtcgg gtcaatgtac ggttcgatgt tcatgccgcc ccagctcgag
    21301 tcaatgagcc cgatcggtat ctttttcccg gttttctcca tgacggtctg cgcgaagaag
    21361 cagccgacgc acgaggccgt gaaccacacc tgccccgtca gcacactcca cttgccctgt
    21421 atctccggcg tttcgcgcgg cacgggcgct ttgtggcgcg ccacgcgcaa atagcggaag
    21481 ttcggcgtgt tcgtgcagaa ggccttcgag aggtccatgg agtacggcca ccagcagaac
    21541 gagtattcca tgttggactg gcccgagcag agccagacat cgcccacgag cacgttcgcg
    21601 aacgtgacgc gctcgccttc gggcgcacag ccgaagagcc ttgcgaagaa gccttgtttt
    21661 tcgcccatcg tcacggtcat cgtccggtca tcgttcgaca cgccgagcgc cggcagcgtc
    21721 gccgcccagc gcccgtccgc gtccacggtt gcccgtgccc gcgcgcccgc gaagtcgacc
    21781 gtcacctctt tccctgcctc gccccttccc cagacgggga tcggcttgtc gcgctggagg
    21841 atcatgttgt ccatgaacac gggattgacc gagagccccg ccagcagaac ggccggcgcg
    21901 cacaccgccg cgacgatcgt caatgtcttc atcgtctcct cagatcattc ttgttcatgg
    21961 cttagagttt gcgaatccgc ttcccgtgca ggccggggat gtgggggttc gccgcgatgg
    22021 gcaacgatca gggtcacgga cgcgtcccgg gcttgaggtc ttgatacaag ggcaccttgt
    22081 cgccgatcca gtgtccgccg caggatttga tttcgcgttc gccgttcagc ttgccgatct
    22141 caacgttctc gagcgtcacc tggcacgtgc cgctgttgcg gatgaggtgt tccgcctccg
    22201 gcacccgggc attgcggacg gtgatcgcgc cctccgcctg atggaagttg aggaagctga
    22261 agaggcggac cgtctcgtcc gcagtgatcc cggatacggt cacgttgtcg aactcggcgt
    22321 cacactgcac ggccagcgcg cccttcgcgc cgggaaggag gtgcaggttc tcgatggtga
    22381 cgttctgcat gggacagaac aggcggattg ggaacccggc gccggaggtg acgttacgga
    22441 tgacgatgtc gcgcatctcg cccatcgcgc agttgcgttt gcggtagtat tcttcagcgc
    22501 tgtcgttgat ctgcacggcg cttgcgttgc gttgccagtt catccaactc ggatcgtact
    22561 cgtcatgcac cccgtcgatg acgacgtcat gcactttcgc gccgtcgcaa cagagaagcc
    22621 gcacctgatt aaaccagagc caagtccccg cacggatgtt gcggacggtg atgtgatgga
    22681 tgtcggtcga cttgccttcg acgatgtctt tcatctcctc gccgcagagg ttggtgagcg
    22741 cgaccacgtc gtcctctgtc cagccggtga tgttctcgac aacgatgtcg tggcagccct
    22801 tgcgcaaatt gacgccgtca ccgtttttca cccaaaggtt cacgtattta ccgggcaagc
    22861 ggttgggccg atgatccctg ccgtcctcgc tcacccaaga gatgtccgcc tcgaagcgca
    22921 gatcgcggat gacgccctgt tcacagaagc tgaagcaggt tgcccagtag cgctgatggc
    22981 ggcatttgag ccccgagacc ttgaaccctt taacgttatg gaagtagacg aagcagttgt
    23041 ggacaagccg ctttccggcg agttcgggcg gcttcgatcg ctcgcccaga ccgttccagt
    23101 tgccgccgtc cagcgtggct ccgttgcggc cctcgatcgc gatatcgcga tcctcggcct
    23161 tcgccgtaca ccggtcgtcc gtccaggcgt gctcgttcgc gaaaagattg caacaaaccc
    23221 cgtccgcctg cacgaggcgc gcgccgtcca ggatcacttt catgccagac gggatcagca
    23281 cggtctcctc gatgatccac ttgtcgtcgt tggccgcgtc attgcgatat ggaatcacca
    23341 cctcgcgccg tcccgccgct tgggcttctg tgatcatttt tgcgatccgc ggcgcatccc
    23401 cgcgcgcggt gtacaacggc ccgccatccg aaccgggtct ggcataggcc ttggtcccgt
    23461 ccgcgaacgt caccgttaca gcgagcccca acatgaccgc aatgcacatt ttcatgatat
    23521 tccgcctctc ttacctcttc gtttctccgg tcggcaagtg gtacgcgcct gtcaccttac
    23581 cgaagcgctt cgtgaacaca taatggggga acaggtcctt gctcgtatgt cccgcatcgt
    23641 gaccgcgatt ctggatgatc gtgatctcgg cgaacgcggt tgtgtcaggg atgccgttga
    23701 acatcgccat catgccgtct gcgggcccgg cagaatcgca gatgccgaat gtgtatgtga
    23761 tgttctgggc ttttgtgcgt gtgatgtggt gcacgggatc ggcgaaatcc gggcccggct
    23821 gccatagggg gcgccagtgc ttttgcgatc cgccgacgtc gacgtaccac gggcacatcg
    23881 accacgcctt cgtgatctgc gggaaaaggc ccgagagcgc gaacgtctgg aatccgccct
    23941 ggctgtcgcc gtgcacatag aggttcgtgc cgtcccaacc cggatggctc atcgcccagc
    24001 ggagcgcacg aatgtcgcgc cgcaacatgt taatgtgata acacgtctcg accttcgcgt
    24061 tctcgttcgt cgggaagccg aagttgccgt ggcgcttacg cgcctcgtcc caataggcgt
    24121 tcgacgcgcg gttcgagatc gaatggcagt tgatgccgag cgtcagctcg ccgctgatcc
    24181 acgtctcggc gaagtggaaa ccggttccat acgcctgcag aaagacgcgg atcggcagct
    24241 ttcccttgcc ttcgcgcacc accttcggca cgccccagtc aaagtgcacc tcgccgccgg
    24301 gcgagtccag cactcccgcg tatacccaca cgttctccgc atcgtttgtg ccgcggcgcc
    24361 ggaagtgttt tggcacgttg gagtatttcg cgtcttcttc gagaaactcc cgccacttcg
    24421 catcgaaacc ctcggggatg ggtttgccgg gcttgatctc cagcgggttg aagagcgcgc
    24481 ccacgcgata gctgagcgtg agcgaatggc gcttgccctt ctcatccacg cgcgcctgcc
    24541 tgagatcacg cacgttcgga tagcgtccgt taagcggttc gggaatgccg cggagcttcg
    24601 ctgtcacggc gacaacgccg ggcttgtcca gcgtgaggat gcgccgcacg gggtttccct
    24661 cctgcgaaag aacgatatcc tcgaccgtcg tctcgtgtgt ccggtaccac gagaccgaca
    24721 tcgtcacgtt cgtgagctgc tctttcgtga gaagtcggcc gtccgccccg cgtacctcga
    24781 aaaccgcctc gggccgctcg ccgacctccc agacgatccg gtcgttcggc acataggccg
    24841 ataacgtcca gggtcgttca taatccgaaa gcccctcggt cacgagcttc gccttcgcga
    24901 actccgccgc catctgcgcc tgcacggccg gcggcagcgg cgctttctcc tgcgcccacg
    24961 ccgcaccggc cagacagatc cccgtcatca acatcatttg tcgtttcatg tccatacccc
    25021 tcgtacactg tgttgctaaa aaaactcgga catgactaca tgatttacat gatgtgcccg
    25081 ttttattcca gtcataatga atcctgtaat cctgtctgaa agcgttgttt tgggttttat
    25141 tgattgctgg ctgtatattc ccgtcaatgc atgtcactcg tccttgaggc cgtccgcctc
    25201 gggagagccg ccggcgagat aaccgcggga ccggcctgag gcgtcggaga accagaatgc
    25261 gtaaagttgc gtatcgcggg cggtgaagcg aagacgaatg gttttgcccg cgtaagagga
    25321 aagtccgccg agaatctccg atttgacggc attcgccgtg aatggcttgg aagccgcaag
    25381 tgcttggcca tcctcatcca gaatctcgac cttaagctcg ccgcccgcgg cattcgcgtt
    25441 cacgaagagg cggtcgcccc gggtgaagcg gatcggacgc gtgacgagtt cgcctccgca
    25501 acggccggca tccattgagg cgaagccgtc ccggcgcagg cgcgcgatac ccatctgcgc
    25561 gagcgtgtaa agccctccga ccggatggtc tttccactcc ccgatctgtc ccgcaaagcc
    25621 cgtgtaatag aagagcagct cgtcgccctt cacaaggcag atcgccgcgt tcgaatgcag
    25681 ataagcgcgg tcccacgcac cgtatgtgcg ggacgcgggt atgaacggcg aacggtcttc
    25741 gggacgcgag aagtggtagc cgtcgcggct gaagccgagg tggagttcgg tgagcttggg
    25801 agagccccga cgctggaact cgctgttgtc cgctccggac atgatcgcgt acacgcccaa
    25861 catcaacgat tcgtaggcga cacaatcgag gttatagagg gacggctcgg gcggttcctt
    25921 ctcgtagttg ccttcctcgc agtagaccca gcgcatgtca cgccgatcac agcagtccgc
    25981 gcgttgccag cagacgcggt tcgtgtaggc gacacccgcg acaaagtcct tgctctcggc
    26041 aagctggcgc gagcgggcag accagaagtc gcgcagcgag tagcaccata ccttacggaa
    26101 cggattatag aagagggtcg agcggtcacc gctccaggca caggcggcgc gccactccca
    26161 gtccgtgccg ttcgcggacg tgaaaatctc gccgccttgg ggacgtgacc aaatgaacgc
    26221 cttgaagcgg ttgccgtccg tcgcgtccgg gtcgaggatg actgcggcgg agtcgcgatg
    26281 acagttcttg ccgtcccgcg tctcgggctt cacgatacgg tttgtcttgc cgtccacctt
    26341 gagcaccggg cgctcccagt tgtagccgtc cttggaatac gcgtacgccg ttccgtcgaa
    26401 ccagcccgcg cagtaccagc acttgtagag cctgtccgtc gcgtcgaacc acacgccgcc
    26461 ggaaaagggc gctgccatcg gcgttttgat cggcccgagc gcaccgcgct cgagcggggt
    26521 ctcgggacgg agcaccgggt tcttcggatc cttctgcgcc ttatgccaag tgcgcaccat
    26581 tgtggtcgag gcgatgagga agtcgtccac gaaaagttga cgcccggcat ccacggggat
    26641 tactgccggg ggattgtcca gatacggcac gggcagcgga tagcgtccgt acttgcgcat
    26701 atcagtccgg tcacgcggat ccgtctcgag gacgatcccg ttatacagcg tctcgcgcgg
    26761 gacgtccttc ttcacggtta cggccggctt gggatcgaac cccgcgatga cgcaccagta
    26821 gtcgagttcg tagcgttcgc cctctttcac ctgtttgcgc cagacgcgga ccttctcctt
    26881 gtcggacgtg ttgaagagct gcgagacggg aatgtcattc acctccgcga acccgagctt
    26941 cttcagttcc gattcttgcg aaggcttgcc gcccttgtcg gtgaactccg caggcggcgt
    27001 cagcgccaaa atttccccgg actgcgtcac ccggaaggag atgccgttga tggatgcgta
    27061 gacaaaggac ttgcccacga gctgcagcgc aacctttttg gaaatgacga aatgccggtc
    27121 gaagaacagc cgctcgccgg gtgcgaattg acggatctcg gaacctgcgc acggcttgat
    27181 tttcgccgtc ggcgcaccgt ctgcggcaaa cccggacaag gcgcagacag cggccgccag
    27241 aacggacagt gtcaggactt tttcaacatt catgttcttc taaggatacc gtttgatttt
    27301 aaggacaccc ccgcgcgtgc gccctccctg aacgcatgcg cggggttttt gtcactcacg
    27361 gggggtcatt tgacgctcat cgtaaagaca agcgggcgga aaagctcgaa acagccgatg
    27421 tcgaccctac cgccttcaac acgcgcgcac ggatcgatct tttcgagttt cacgcccaca
    27481 gggaaagtct cgccgaacgc cgtgatcgtg gctggatggg tctcccggaa accgttgccc
    27541 atgtccagcg ccccttccgt catccacgca aggttggtgc ctgcgttgta acacgccgaa
    27601 cgccgcgtaa gcctgtaatc gccctgcgca ctgtccctga acatcggatc cgacccgatc
    27661 acattcccgt ctttgtcgaa actcgcggca ttcgaggcgc gcgaatcgat aaagcagtgc
    27721 cgcaccacgt tcgttcccgc gtaccagtcc tccagcgcgc ggctcgtcgt cgtgtcctca
    27781 aggcagttgt tgtagataag gttgttgcgc tggaaaagat catattggct gcccttaagc
    27841 gcatacgacg aacccaaaag cgtgttgtcg gcgatcgtgc agttctcgac tctcccgcaa
    27901 gcgccctccc ccgctgcaat cccggattgg gcgacattcc actccaaaat atacagcaca
    27961 tgtttcaccg tattgctgac gaaaagacag ctccgtaccg tcgcgttcga cgtgccgaac
    28021 gcgtaaaacg ccgcgccatc ggatgtgctg ctgttgttga tgaacacgca gtccgacaaa
    28081 aacgtcgata gtctctggtt gccataataa acgacaccgc caccgtattt cccggcatag
    28141 gtgcacaggt tgctgtcgaa caggcagttc gagaccacgg ttccgccgtg gctctgcatc
    28201 gctgagccca atatccccga gttgttccgg aacgcgcaac cgtgcgcgac accgttttgg
    28261 tcgaggtata aaccgccgct gccgcactcg ttgccgccgc cgggcaggtc attgccctcg
    28321 aagtaacagt cgaaagccgc tattccatgc attgcggcgc ccgcctgcgc gctgccgatg
    28381 ttgccgatga aggagttgtt gccgcaggcg tataccctgt cgaggaaagc gcccgagccc
    28441 ttgccggtga tgtagttgcc ttcgaaatag caattcgaga ccgtggtcag ggccgtaaac
    28501 aacccgccgc cgcggcagtt gtaaatctcg agcgggttga cgagtcggtt gctgataaag
    28561 gcgcagtcgt accaactgcc gccatgcgcg ccgccgcctt gcataaagtt atatctggcc
    28621 acaccacggg tgcagactgc ggaacagttg cggatgatcg aacggcgtcc cgtggagaga
    28681 ccttgcacac cgccgccatt gcatgatgtc gcattcgtgt aggcttctgt cacggtgttg
    28741 gagatgcagc agtgctcgac gagcgaatca atgagatccc ccccgttcgc tttgatgccg
    28801 ccgccggcaa ttccaatctc gcctgtcccg ataacggcgc aactggtgac gatgcagttt
    28861 gatatgaccg cgccgccctt cgtaaggcac aggccgccgc cgccataggc gctcgtcacc
    28921 tcgcaacggc cgttcgtgaa ggtcagatta tggaagtgcg agttgaaata tgtgctcgtc
    28981 acgcagttcg tgagttgctg tgcgtcaatg accgtattcg cggcgacccc gtcgtcaccg
    29041 acaagcgaaa caccgtctgc cttgaatgtc aagggggctg aaatgagata cgaccccttc
    29101 gcgacatgga cgatatcatt cgccgaggct gcgtcgagtc cttcttggat tgtcagaaaa
    29161 ggcgtcgcct ggccgccgtc gtgcgtgtcg gccgtcgagt ctttgtcgac ataccactct
    29221 gccgcctgaa ctgccgctcc caagattgta gcggcaacga tgaccgtggc cctgaacgcc
    29281 tttgttttca tcatcactcc tcttatttca cttgacatac atgcccgtgc ctaagggcgc
    29341 ccaggattcg aaacagccga tatccggcac ggtcgggaag atgcggggca acgcaggaac
    29401 gcgcaccaca tcgcatccgc cttgcgtcag cgtttcgtcc cacaccaccg cctgattgga
    29461 aagatcgttc gccccggcca tccacggctg cgctttaccc gcgtttttaa gcggagagcg
    29521 gcccttcaaa cggaaatcgc ccttggcggg atcggcaaac ccggggtcga tctcggtgat
    29581 gttgccgctc aacacgttcg tcgtgaacgc gtcgaccgcg atcgcctgcg gcccgatata
    29641 cgagtgcgaa gtctgcgccg ccatgctcgc attcaggtta tagttgtcag ccgtcccggc
    29701 cttataattt ccccacaccg ccgtgttgca cacgtaaaac ttcttgcccg tttgggcaag
    29761 aatggcttca ctcgtgccgc tcaggcggtt gcccgcgatc gtgcagctct ccacccgcgt
    29821 tccggggaac tcctgcccga tgtccgcgac gggattggag atgtccagaa cccgtgaaga
    29881 ggcgttatcg taaaaaaagc agttgcgcac cacggagttg gtcgttgatc cgacctcgtt
    29941 ttcgcgtccg ctctgcctga caagcggttg attcccgtta tttccgcttt ggttgccgat
    30001 gaacgaacaa tccacaaggg agaagttcag gccggagtta atcgccaaga gggcgtgtgt
    30061 gccgctgttg gtggcgctca ggttggcctc aaaatagcag ttcgaaaggg aaacgtacgc
    30121 gccgccgttt ccagaccaag tggagagagc cgccatgcca aagtctttgt tcacgtttcc
    30181 gacaaacgcg cagtcatgga taaagaggcc ccgatacccc ccctgcgtat cattcaggtg
    30241 cgcgtgaacc gctgtgccgg aaccgtcagc aaccccgcct ggcgagtaat tgtccttaaa
    30301 aaggcaatgc cggataaccg cttcatttcc tgccccaagt tttaggcttg tcgtcaaaga
    30361 agcgccgtag cgctccgtcc ggttgccgac aaaggtgcaa tgcgtaaaca gcgcgggagc
    30421 gctggcattg acacagaccg gttgggagac cgacgaattc ccgacgaaaa ccagattgga
    30481 gaaaactttg ccgctcccct cggtaaagac ggcgccgccc cttcccgccg ctttgtcggt
    30541 tctgctgctc caaacgcggt tggtctcaaa ccggcaatcc gccgtcagtg tcccgacgcc
    30601 atgcattgcg ccgccgtcca ggtcccccca tttccccgtc ggatcgacaa tgctgttgcg
    30661 gcggaagatg ttgccgcgaa actccttgta gccgccgccg catatcgctc cgccgcggaa
    30721 acggaggcct gtgagggttt gcgactctgc ggacgcacag gcgttggtga tgccgttgtc
    30781 ctcgaagagg gtgtcgacga ccgacccgtt cgccacagcg accgcaccgc cgtaaatgca
    30841 ccccgccgta ccgccttcgc ggaaaccgcc cgaccagcag ttccggataa tgcaattcga
    30901 gataaccgca tcagagttgc gtgcgttgat ggcggaaccg ccatagagct ccaggccggt
    30961 tgcggcaaag acatctttcc ggcaattgac gatggtgagc ccgtgaaact gcgagccttt
    31021 ggcatacccc tgaatcgcct caaaagcgtt ggtggcgttt tgcccgtcga tgaccacctg
    31081 ctgggcaaac ccggggttcg ccttcagcag gacattggtg acaccgtaga acgtgaccgg
    31141 gtttgaaccg ggaatagcag gaatcggctc ggacaaagca tagtgtccgg gcgcgatgta
    31201 tatggcgctc ccgctctcat aattattcgt aagcaaattg ctgacagcct cgccgatggt
    31261 cggatacgcg gtttcccacg tcatgccgtc gggcatgacc gcagaagtgt tcagatccac
    31321 gtacaccgtc ctggcccgca ccgcgagggc gcacacactc aaaacaacaa ccagccctac
    31381 atttctcatg atgaatctcc ccccatcacc acctattatg tgggggaggg gcgaaaagag
    31441 tcaatcctga aagcccgcgc caggcgaaaa agggggaggg ggaacgaatg aaactcgttt
    31501 gacaggcgac tgtactcttt cgcataatgc gagtggaatc gattgacgga gggacatatg
    31561 aaagacggtg tcagattggt gtgtgcgctg gcaatgccgg cgtggatcct gcacgcggag
    31621 cagccgtgcg agaattggct gtcgccctac acgggcgcgg atgcgaccgg cgagaaggtg
    31681 gtggggctgt ggactttcgc agacttgaag gatgcatcgg gtagaggacg cgacgcggtg
    31741 ctgcacggtg ccgtgctggt gccggacgcc cgttggggtt cctgcctgga gtcgttctgc
    31801 ggctggccgg tggaagacaa gcgtcacgcg gctgttgtcg ccaatcacac ctcgctctcg
    31861 cctcgggatg cgttcacgct ggagatgtgg atcaagccga agcctgagtt tgtcgattat
    31921 cccgaagcgt tcctgatcga caagaagtac gtggcgcaca cggattacca atggacgctg
    31981 ggagccgccg ataaaagcgg tcagcggcgc gcctatctgc ggctcggttt cggcgatgat
    32041 tcggaagtgt atagttcgga gccctcggtg tttgcgacga acatctggac gcatctcgcg
    32101 gtcacctatg acggcgccgg caccgtgcgg tttttcaggg acggcgccgc gcagggtacg
    32161 gtcacgcgga cgggacgcgg gcgcatcgcg ccgggcagtc atccgctctc catcggtgac
    32221 cgcatcggca gctactatca cggctttccg gggtatctga cgcaggtgcg gctgtgtcaa
    32281 ggcgcgctgg agttccgtcc ggtcacattg gccctgacat ccgaccggag cgtgtttgtg
    32341 cgcatggaaa agacgccgtc cttacgtttc acgctggcca atgcccaacg cgaagccgcg
    32401 cgcaacgtct cagtgttctg tacggtcgaa ggacgcgaag ccgcgcgcca tcagatcccg
    32461 gaactcgcgt cgggcgagag ccggacgatc gaggtgccgg tcgacacggc gttgcgtccc
    32521 ggtagctaca cgctgcgact gcgcgtcgag acccccttga aagaaacgac agccccctat
    32581 gtgagcgagg agagcgtaac gttcaccttg atgccgcgcc gtccgccgcg catgccggtg
    32641 gtgatgtggg gcctttacgg agcgggcaac gtcattcgcg agttgccgcg cctgacggaa
    32701 atcgggttca cgcactgttt ggggttcgga gcggatggca agcgtatctt cgaagcggga
    32761 cagccggtcg cgcccggaac acctgagcac gtcgcggagg tcaagcggat gctggacacg
    32821 gcattggcgc atgatgtcgg catcatcgcg acgctttctc ccggccactg ggtggccaac
    32881 gccaagccgg agttccggcg tgtggatcgc ctgggcgtca gtaacgataa cagaccgacg
    32941 ctctgtgcgg cgtttcccga aatgcagacg ttcgccttta acgtgggcgc atcggtggcg
    33001 caggcctaca gcgactttcc ggcttggcag gcggctctgc tgcataccga ggtgcgtgac
    33061 ggtgcgaacg tctgctggca cgcgcatgac cgcgaggcgt gtcagcgcgc gaccggttct
    33121 gactatccaa cgaatgcacc gaataaaaac ggcgtggact acaccaagct gagcgatttt
    33181 ccgtccgaca gagtgatccc tgatgatcat ccgctcttgg gtttcctctc ttggtactgg
    33241 aaagagggcg acggctggaa ccgcatgaac tccgaactgc accggggact ccagtcgacc
    33301 ggtcgggaag ggctttggac gttccacgat ccggcggtac gcgtggcgag cgtctacgga
    33361 agcggcggga gcgcggatgt cctgtcgcag tggacctact cctatcccga cccgatccgc
    33421 atcggagtgg cgaccgacga actgtttgcc atgacgcgcg gcgccgctca gccgcagcag
    33481 gtcatgaaga tgacccaaat catctggtac cgctcgcaga cggcaccgat cgccaagggg
    33541 gacgaggtca gaaaagcgcc cgcctcgccg tgggaggaca ccgatcccga cgcggcattc
    33601 ccgactattg cgcccatgca tctgcgcgaa gcgttctgga ccaagctggc cagaccggtg
    33661 cgcggcatca tgtatcacgg ctggggatcg ctggtgccgc agagcggtga gggctcctac
    33721 cgctacaccc atccgcagac acagcatgag ttgcgccgcc tgatcaaaga ggtcgtggag
    33781 ccgctggggc ccaccttgct gcaagtcccc gaccggccca gcgatgtcgc gtttctggag
    33841 agcttcgcgt cgcagatgtt cgcacggcgc ggaacttacg gctggggcgg cgggtggacc
    33901 ggcgacgcct atcagatgct gctgtgggcg catcttcagc ctgagatcat ctacgatgaa
    33961 acggtgaagg cgcatggact gggcaagtac cgtgtgctgg tcatgatgga ctgcgatgtg
    34021 ttgacagccg gcgtggtggc tgcggttaag gcatttcagg cgcggggcgg ggtggtggtg
    34081 ggggatgaac gcctctgccc ggctatccgc gctgacatca cgatcgcgca ttacacgcgg
    34141 acgcgcaaag cggataaaga ccgcgccgct ttgatcgcta aatctgccga gctgcgtacg
    34201 gcgatcgaca cgcgttacca gcgttattgc gatgcctcaa cgccggacgt cgtgccccgc
    34261 tgccgttcat acggctccac cgattatctc ttcgccgtca acgaccggcg tgagttcggc
    34321 gattacgtcg ggcagcatgg tctggtgatg gagaatggct tgccggcaga ggccgtgctg
    34381 tcgttggcac gcgcctcggg tcatgtgtac gacctgacag aagggcgcac cttggaagcg
    34441 catgtgcagg acgggcggct aactgttgcg cgcgtcttgg gtccttgcga cggctgtgtg
    34501 ctgatggtca cggaacgcgc cattgatgca gtgcgtatcg acgtcgcgca agaagcgcgc
    34561 ctcggcagcc gtctgccctg cacattttcc gtgatggatc gagagggaaa gccgatcgat
    34621 gccgtggtgc cgctgcatgt cgagattctc gatccggccg gcgcgcaagc ggagtggagc
    34681 gggcattacg gtgccgaacg cgggcaactc gcgctcacgc tagatctggc ctcgaatgac
    34741 cggcgcggcc tgtggcaggt ccgtgtgcga gagctggctt cgcgccgctg cgccacaact
    34801 tattttagga tagtgaataa cgaaaccgga aaccaacgag cggtatgcct cattcagagc
    34861 cctatctttg tgcgcccgga tggcgtgggg atatcggcct tcatcccctc cgctccggga
    34921 atgggcttta cctcgtgcgt tttccagccg tccgatgtgg gcttaacccc catcgcctcg
    34981 gaaacgaaaa tgaacgcggg ccacgcgctc catgcgtggc aaaggctttt cgcccacggc
    35041 cgcccgtaaa aacgatatcg ctcatcgcct ttctgtttgg cgttaaaccc ttcccagaaa
    35101 ctcgtcgcgc ccgcatcaag catcgcgccg aacacggttt ccatgcccgt gaaaaactcc
    35161 ttatggtgtc cggtgcgcgc caaggccaca agttcccatc cgaacacgta aggcgtgcct
    35221 accgccggca agtcatcttt ggcgagttcg tcgccgatcg cctgcgcccg ttcattctgg
    35281 gccacgccga agatcaccgc gtaaatattg gcctgacgcg caaaccccga attcttctca
    35341 aacggatcga cgcggtaaag tttgcgccct tcgtcccacg cgagacggtt gaggttcgct
    35401 ttcactttgt cgcaaagcgc acggtactca gcggcacgcg ggtcgtttat gcgcccggcg
    35461 agcttcgccg ccgcgtcaag tgctcctgtc catatcatcc ctagtgcggt agcggacctt
    35521 ggaccagccc aatcgatgaa cacccagtcg agccctcttg cgacaaagcc gttttcatcg
    35581 gtgcgggaaa tcaggtcttc gatgcgttgc ttgatccgcc accaacgcgc ctccagaaat
    35641 tcgcggtccg cgaaataaag ctgatgcaga tcgtgcgcga tgatcagcca catcgaatag
    35701 gtcacgatgc cgttcacgtc cgttgtgtat gcgtccagca cagaaagcga gcgccgggag
    35761 atttccgggt cacccagaac atacgcattc gccatcagcg acaccgtgag atcacctccc
    35821 cacggcagac ggtcgcgttt gatgccgtcg atcaaaaagt cgtggcagca aagcttgagc
    35881 gtgtggaccc ccacctcgcg cattttctcg tgacgtttat ccttcgcgga aaacgttcct
    35941 ttttcctcca tgcccggcca ccacagcggg atcaccttga ccttttcggc ttttccctca
    36001 aagcgaaaat atctgagcgc gagcgcggac ctggatcgcc acgtgtcgtt ggcgaccaac
    36061 accatgtctg tcgcctgctc tgaatcgacc gggaccgtat tcctcatttc cggcacggat
    36121 tcgccgacaa aaagtttcgg cggcttttcc ccacttgtct gcacataggc aagcaattcc
    36181 ctttgcgtgt cgtaataatc cccgattttc ttcaaatccc gatagtcgtg atcttcttca
    36241 agccacctta aatgcggcgg cacctcgccg ccggaataac gcgcggcagc gcgccacgtg
    36301 tcgtcccgtt gcggaccgga caccctcgcc tcccaaccct tgagcggact gcgcaatgcc
    36361 ggcgtgtcga gataccgtgc gctcaccgag acacggagca tgccggtctt ttcgtcgaag
    36421 ttttcgctga acgcgcgtcc gtgcgtgaaa tattcgcgcg tttcgcctgg cttgaaacga
    36481 cgccggaact ctacggctgc cgcgtacggg ggcacgctgc ccggatatcc cacatacatc
    36541 ccggcggcct gcgcattccg tttcagttct tcgagccggt acaactccat gctcatctgc
    36601 ggataatacc aggcgtcaca cccaccgcct ccgcgcggag aactccccgc gtccgcaccg
    36661 taaagcgcgc tggaacattg cgcagaaatg cacacgcccg ccacccaaac cgacaccgcc
    36721 gcaaccgtta acctgcccat cgtctttccc cttaaacaaa cataagcgag cgccgtgacg
    36781 ccgcagtcag acccgcatcg acctgaccgc ccgcttgacc gccatcggct ccgggaccga
    36841 tccgtccgcc atcgcgttat ccatgcggta agattgcaac tttgcagcgg catggcaagc
    36901 ttgaagagtt ttgcaacaat tttgcttgcc gcaaaccttg tcaatcccat accctagtct
    36961 ggcaagttaa taccttgtat agtatagtca tcggcacgac gatggggttg cggtcgagtg
    37021 atcgcggaaa ggaatcgggg ttaaatgcgt cttgatgcga ttgtgaggtt gctgttgacg
    37081 gtttcgttcg ttgcgcacgt caggtgtttc gccggaccgg tcgtgacgcc gttcatggaa
    37141 agaacgaggc cgggggcggt aagacccgag ggctggcttt tggacatggc gcggaaagcg
    37201 cgcgacggat acaccggtca catggacgcg gtggacgttc agttctcacg ctcttggaga
    37261 aatgaaacca ggccgcgcgg aaaggatctc ctctgggtat ccgatcccgg agcctggagt
    37321 tgtgagggcg gggcgtactg gtttgacggg ctggtgaagc tcgcgtatca gttggatgat
    37381 ccgtcgttga aggctctcgc atccaatcgt ctggataccg ttctctcgga gatgaacccg
    37441 catgccatcg gattttgctg gtggctggat cgcaacgatc cccagcaggt tttggagatg
    37501 aaaatttcgg atgcgtggtt gatctgggtg acggggatgt ttgcgcgtcc ggtttgcgct
    37561 tggcacgagg tcagcgggga tgcccgggcg ctcaaggcgc ttgacttcgc gcttgacgaa
    37621 cacgcttttt cctacggcaa gtttcctacg ttgccctccg gtgcatatgc cggttatcag
    37681 gtgacgcgga gcgagcgtat tgcccgggcg ctcgatgaat tctggaaaaa ggcgcttcac
    37741 cagcccggtc agcttccggc ggttttcagc caatatttga cgccgccccc cgcgcatttg
    37801 gaggagacgc taaacatccg tcgccttcac gagcgtttac tgaagatccc tacgcgtcac
    37861 ggtgtcatcg cctcggaggc cctgctgtcg attttcgacg gatacctatg gaccggcaat
    37921 accaatctcc tgaacgcggt ccgcggctgg tacgcctttt tcgaccggca catgcggcaa
    37981 ccctacggtg tcaccacgat ggacgaggag tgggggcatc ccgggccgga acggggtacg
    38041 gagacctgcg tcgtggcggc agagggctgg acgcgtatca accttctggc ggcactgggc
    38101 gaaggatgtt ggggcgatga cgtcgaacga aatttcttta acgccggacg gaactgcatg
    38161 acggaggatt accgtcggca cgtctacctg caacaaccga accgccttac ggcaagcgat
    38221 gtcacgcgct gttcgtttac gggtgcccgc ggcgaccggc tggcgcgtta cgacacgaaa
    38281 cattggccgc tttgctgtac ggcggcgctc aaccgtattc tccccaacta cgtcgcgtcg
    38341 atgtggatgc tgcctgcgga cggcggggtc gcggcgacac tttacggccc gtgccggttt
    38401 gccgcggaac tgtcaagcgg ctcgtttgag gtggaggaac acacagacta tccgtacagc
    38461 gatacgatca ccttcaagat cgtcaaatca ccgaatgcgg cgttcccgtt tcatgtgcga
    38521 tttcccggat ggtgcgccaa tccagaactg caagtcaacg gcagacaaac ggtgatcgcc
    38581 gcacagaacg ggttcgcggc aatcacgcgc atctggcgtg ctggcgacgt ggtgacgttg
    38641 aggctgccga tgaaaccgac ggtggagaag atgttcaact acaacgagcc gcgaggacgc
    38701 gagtacgcca gcgtgtcggt cgggccaatc ctctatgcgt tgccgatctc ttcaaaggac
    38761 gacaacaatc ccgaaggtga tgtgcacgtc ccattggtct cggccggttt cgatgccgcg
    38821 tcggtcaggg ttggcgatgc gatgcgcatg ttgacgatcc cggattcgaa gggaaacagc
    38881 ctcaagcttg tgccatatgg ggacgcgaag ctgcgcatct cgatgtttcc ggttgacggc
    38941 gattgagtgc gcgaatctgg gtctcggggt tcgaaccatg aagcacatga aacttcatga
    39001 aaccaaaacg acgtctctcc gaggcacccg caggttggct gtttgacttg tgttgcgaat
    39061 ggtgtaaact gtaaaattca atctgcttaa gagggggaat gcggaatgac aaccgcaacg
    39121 ctggctgctg gctggcgggg gggtaaaaga ggaaataaaa acaggagcaa agacatgaaa
    39181 gagacaaacg tgattcgtgt tcttccggcg ctcggctgcg gattgctcgc cttcgccgct
    39241 tcggcggtcg aaataaacgt ggcgtcggtc accgatctgc gcgcggcgat cgccgatacg
    39301 gtgaacgtgc cggatggctc gaccatcgtc ctgcaggcgt ccccgacgca ctacgatgtc
    39361 tcggccgacg gcctcgggcc gatcaggctc acgcgctcgc tgacgctgag gggctcgacg
    39421 gagaatccgc gcgacacggt cctcacgggc aacggcatga ccaatatcct ctatgtgggc
    39481 ggcaccgata cgctcgcctg caacctcacg gtgacgaacg ggctctccta tctggtcgat
    39541 tcccgcaagt ggtaccgcgg cggcgggatc catgtgaacg gcagctcggc cgttgtctcg
    39601 aactgcgtcg tgaccggctg ccacttcgat acgcgtaccg actacctcac gggcacagcc
    39661 gtcgccgaga tgaccgcgtt cggcggtgcg ggcgtttacg cgaacggcag ctcgctcgtc
    39721 ctcgacacac tgatcgagaa ctgctggatg ttcaacgata ggaagcagaa ggtcacgggc
    39781 gccggcgccg gacttttcat cggtggcaaa gcctggggca ccgatacgcc atcgcgcgct
    39841 tatcgttgcg aggtgcggaa ctgttcgacc gagacaatgt cggtcggcgg cggtacggga
    39901 gcactcgtgg gcggcggcct gtatgtgagc gacgcgggca tcgtcgacaa ttgccgcgtc
    39961 caccacaact gggtccggcc gacggatgac agctcccgct gctatggcgg ggggctctac
    40021 gtgggaaccg gtggaatcgt cagcaattcg ctcgtttacc tgaacacatg ccaaaaatgc
    40081 gtgacgaaca cgagcggcaa cgcctccggc ggcggcgtgg cgatcgaggg cgccggactt
    40141 ttctgcacat cgaccgtttc gaacaactat tcgtacggcg atgccggcgg agtcttccac
    40201 atcaactatc cctcgggcgg tctgcgcgac tcgttgatca ttgacaacga agccgtcgga
    40261 tcgggcggcg ccggcacggg ggggcctgcg gcgacggggt gcgtcttcca ccggaacacc
    40321 tccggcggcg gcggaggagg atggctcacg ggcggttcgg cgggctggtt ctcgaactgc
    40381 gtcttcgagg cgaatgcgac gggaaagtgg ggcgagggga aggccgcgcg tgtccagcgc
    40441 ggcggttcga ccccgttcgt gttcgaaagc tgctggttcg tcaatcacac ggtgacggac
    40501 ggcaaggcgg tccttcagtt cgacggttcg cctcttccaa cctaccgcgc gcacatccgc
    40561 aactgcgtct tcgtcgggaa cacggggtcg tgcccgttct attcgtctta caccgccacc
    40621 gggccggaga tcgacaactg cacctttgtc gcctcggaca cggaatacta cgccatcgga
    40681 gcggggagta agcgcgagac gaatgtcgtc gtccgcaaca cgcttttcca cggttacaag
    40741 aacggcggcc tcccgccggc cttcgcgacc gacaagccgg agcatgtctc gaactgctac
    40801 gatcaggtcg cgacgacgct cccgaaggac ggggacggca acatcctcaa cggcaacctc
    40861 gcgggaacga tcgtcggcga gccggggttc cggaatctcg ctgagacgaa cctgacgctc
    40921 cgcggggcgt cgccgctccg ggggaagggg ctcgtcctcg actggatgaa gacccaggac
    40981 ggttcggctg actggacggc gtgcgatgcg ggtgcgggca cctatatgat cgaaaaggtc
    41041 gcagactacg gtgtcgcggt cagattcggc cgacgttacc cgcgctacac gggcggcgcg
    41101 gacgctcccg acatcggcgc gttcgagcat tacggtacgg ccgacgtcat gtatatcaag
    41161 taggaatcgg gaaacaggga cgcggaatgg ggtgaagaga agtctggcga tggggctggc
    41221 gctcgcggct gttctgccgt gcgtcgaagc ggggatgaaa acgattctcg acgtgccggt
    41281 cgcgggcgtc acgaacacgg cgtatctgac gggcatggcc tcccgcccgt ggtggaacgc
    41341 ggcctggaag cgccgcgcgc cgattctcgt ctcttcccat gcggatgtcc cggagccgaa
    41401 ggcggtcgtc gattgcgtgg tcgacttcgg cgagaaggtg aacccggacg aagtgcgcgt
    41461 ggtgacgcct tgggagacgg tcgtgccgtg cgtttgcgag aagggcaaag cggaaggtca
    41521 aggccaagaa cctgcggccg tcaggctgct tttccagacg ccgctcagga tccacgagaa
    41581 caagccgttc ctcgtctact ggggcaatcc ggcggcgaag cgcgagacac cggtaacggc
    41641 gctgatgatg tccgagactg cgaacgaggt gcgcattctg aatggcgtgc tggatgtcac
    41701 gatcgacaag aagcatgtga cgggcggatt cctcaggaag ctgcgcatca tgggcacgga
    41761 cgcctcgaat gagctgctcg agcgtgcgac ccagtgggcg tggaacgggc ttgagatgac
    41821 gttgggcggc gcgacggact ggtccgacgg cgtcgtcacc gaggacaacg ccttcgtgaa
    41881 gcgcgtccgc ttcgagggga cgaacggcgc ggtcacgctt tcggtctacg ctgaccagcc
    41941 gcgcgtcgac tgggcgtacg cgctgaagaa gggcgaccgc tgcgaactca acgtcgcctg
    42001 ggcggtgggc ggcggcgtcg cctacgacaa gttcgtctat ccgggcgcga ccggcaggct
    42061 cctcacgttc gatgctgcgc tggacagccc gaccgactcg atcccgcagc cccgcttcga
    42121 gatggcgccg tggttcggcg aaggttggta cgcgatcgcc tcgaagcggt cgcccagcgt
    42181 ctgcgggctt ctcttcgacc gtcccgcgct gcaggggctc tggtataacg gcagcggaca
    42241 ggccttcggc gaatggacgc gccttacctt tgcgcacaag gcgcggaagg acgcgccggt
    42301 gaccggttcg ggttcgctcg tggcgcttcc gggccaggcg ctcgacgtgc ggcgcgaata
    42361 cctgcggcgg aaaacgccgc tgacggtgtc cgtcggtgcg gcgcagccgt acgcggaata
    42421 tccgtacgtg cggccgcgcc tcgaccgcga cttcatcgcg gatatcaact gtggcaagga
    42481 ttcgggccac ggctggcggt cgggcaagcc gctgccggat ccggcgtggg cgtcgaacat
    42541 cgtcgaccac ctgcggtcgt acggggtgac gggtatccgc gtcggcgggt acttttggtg
    42601 ggacatgccg ctgacggaga agctctaccg cgacctcgag cggcaggcga accgtccgct
    42661 cgtcgcaccg gactgccgca agcgtttcac gggctgggat ccggatcagt atcgcggcac
    42721 ggacttcctc gagatggcga acgccgcgca cgcgaaggga ctcgtcgtca agcagtgcgg
    42781 tgacttcatc cccggtatgt acgggcgcga gttcgacacc aagttcgacg ccgatatcca
    42841 gaggacggcg ctcgatctgc acctccggat ggccgaggtc ggcggcgtgg acgtcatcta
    42901 caacaacttc gagggccagg agcaggtcat cctgccccag gacgtgaagc gcgagaagca
    42961 caacgactac tggaactgga aggacccaga cgaattcttc gcctggcagg acaaggcccg
    43021 cgtgctcgtc aaggcgttct acgacgcggc gaagaaggcg cggccggacc tcccggttct
    43081 cgtctacaat tcggagaacg gcgaactgag ccgtgagatc ttcatgtccg agcagatcgg
    43141 cagtttcgac acggtcgtcg tcgagatgct ctcggggctc gacttctcgc gctgcaagca
    43201 cgtcgccaag cgcatgcgtg cgctcttcga caacgaagcg gggcggacgg tccatcacca
    43261 ctattacttc atgaagccgg acgcgatgca ccgcatcaag gagatcgagc tgccgttcat
    43321 atgcggcgtg aacggcttca gccacgagaa ccagagctac gagaacttct cgcgcgaaca
    43381 gagcgagatc gcggccgact tctaccgctt cgccgagtat acgcgcctcg gcgagaaggc
    43441 cgcgcggatg gcgcccgtcc gttacctcgg cgtcttccgc gattccgtct gctaccgcga
    43501 ggacatccgc aagggccgtc acggacagcc aaaagaccgg ccctacttca accgtgccga
    43561 gcaggatggc cgctgccgcg cactgggcga gatccgcaac ctcgacttcg actttgtgat
    43621 gaacccgttc ttcacggcga aggcgctggc gaagtaccgg atcgtctacg tgccggagga
    43681 cgacgtcttc tcggacgcgc tcgccaagga acttgtcgcc tacgtgaagg ctggcggaac
    43741 ggccattctc gaagcgaaaa ccgccgggaa caccgtcatc aagtcgcttg gcctcaagga
    43801 cggcgtgatc acgccgctcg gcaagggtaa gatcctctgg tatgagaaag tccggacgga
    43861 cggacttgcg aaacgggatg agaagacgat tcgcgaggtt gccgccgcca tcgaggctct
    43921 ggccgggccg atgccgcacc gcatagacgg cgcttcgtcg ctcgacggtg tgttgcagtc
    43981 cggttccgac ggaatgctgc tcggtgtcca caatgcgggc gacaaggccg acgcgggacg
    44041 ggtgacgatc gcgaaagcgt tgaggtcggg accttctctc tacgtcctcg acgtgaagac
    44101 gggtgtgcgc ttcccctaca cgaacggttt cgacatcgcg atcgggccgc gacagtgcgg
    44161 cttctacctg atcggcgatg aaacgttcac ggcggttccc gaaacgaaga cggccgtctg
    44221 gaacggccct gcggttgccg ccgcgcgtcc gaacggaatc ccggcccagg tgaacgatga
    44281 cccttcgttc gtcccggcga aggctgtcga attcacgcgc ggcgatggga agggaaaccc
    44341 gaccgcgatc cgccgttcgc gcgaggcgat gatcgatgtg acgcccgtga cgccggccga
    44401 ctgctatccg cccgtcctcg cccgggccct ctcaacagcg acctacatcc atttctcctc
    44461 gctcggcacg ctttccgcag atgaaaagaa gaccgtcgac gacgtcttcg tcagttgcgc
    44521 cgacgagctg aaagcgctcc tgaagcgcgg cgggacgatc ctcttcgacc gctccccggc
    44581 cggaccgcag acgcgcgcgt ttctgaagga gatcggcgtc ttcgacccgt acgcgcatcc
    44641 cgcgccgaag ctgggcgacg agaacacggt ctggacatcg gagacgaacc atccgttcgc
    44701 cgggaatctc gccgtgaaga agggcggctt cggccggttt caggccaccg agcgctcatt
    44761 cgcgaagtgg gatgccgcgc gtcaggtcgc ccccgtccgg atggtacgca atcacgatgt
    44821 ggccgtcttc gtccttcagg agaacgtcct cggcacgggc cgcgtcgtct tcctcgagaa
    44881 caggttcgcg ttctccgatt ggtacgagaa ccgcgcgttc ggagatgcgc tgctcggctg
    44941 gatcgttggc cggccgatcg aagaacacgc taagaagtcc ttgcaacgtg ccggcggcat
    45001 cggcgttgct ttatcggaat gacagaaagg aagcggatga tgaaacacat gttgttgctg
    45061 acggcttgtg cgttgacagg ggctcgtctt ctgccagctt tcggtgatgt gacgcctgcg
    45121 gccttgccgg gcggctacac cgagctgccg tatgtcgaat cgacttacaa cggcggccag
    45181 tgggtctata cggactatgt gccggaatgg aatcaccgta tcgtttgcaa gttccgcgtc
    45241 aatcaggtcg gcacttggaa ctcgcccggt gtcttctgcg cacgcggcac agcgaccaat
    45301 gacaaaggtt actgtctcag cttcgggcag ggcggcaaca accgcgtgaa tctcccgggc
    45361 gcccttcagt gttggacggg cggctgggcc gcgccgtcct ccgcgccgtt tttctatccc
    45421 gatcttctga ccgactacga ggtcgacgtc aacgttgaac tcaagaagtg gtgcgtcgat
    45481 gacggggaac gctggaacac gcgcgagcac acttcgttca cggcggggag cccgctcgcg
    45541 atcttctgca ggcacgagct gggcaactcg ctttcgcctt cttcgacctt cggcgccaac
    45601 aactgcatgg cgggccgcat ctactacctg cgcgcgtacg acgcgaacgg aacgctgatc
    45661 cacgaatttg tgccggcgcg cgatgacaac gcgatggtgc cgacgaagta cttcggcttt
    45721 tatgatacgg tggacgccaa attctggccg aactgtggcg cgacggcgtt cgcgaccgat
    45781 ccttccgtac cgtccaacgt gcgtgtcgtc gaccgcgccg attacgatac agacgaggag
    45841 gcgggcacgg cgctcgtcgc gacggttgcc gccgcgttcc cgaccgaccg gatcaaggtg
    45901 acgggcggga cgaaggccgc gccgcacgtc taccggatca cctcgcccct cctgatggag
    45961 atgacgaaca cccggctctt cggcgactcg gacgatgccg aggcgacggt gatcgacgcg
    46021 ggcggaacat cggcgggcgt cgttgtgaag ggctccggca tctccgtttc ggggctgacg
    46081 gtgaggaacg cgaccaacta cgtgaacgga acgacgtact gcggcggcgg cctcgcgctc
    46141 atcgcggagg gttcggtcgc gagcaacatg atcgtctgcg gctgcgtcaa cggcggcgtg
    46201 gagaaggctc tgcgcggcgg cggggtctat ctttacaagt cgacgctcgt cgacgcgctc
    46261 gtgaaggatt gcgtcatctc gaacgacatg gacacaacgg gcaagacggc gttctacggc
    46321 ggcggcgtct acgccgagcg ctcgaccgtt tcgcgccttg aggtcagcgg ctgcctcgcg
    46381 gtcaacaacc agatgtgcga atcgatgtgg tgggcgaccg gcgctttccg tggcggcggc
    46441 ctgatgctca acgaatcgtc ggcgaccgac tgctacattc acaacaatat gatcacgaac
    46501 gggccgaagc agacgtcgag ctgcaacgtc agccatggcg ctggcgtcgc gctaatgggt
    46561 gcgtcgacgc tttcccgctc gctcgtcacg ggcaattccg ccccgtgctc tgccgcaggc
    46621 gtcaacttgg agtcggcgtc ggccgtcatt gactgcacga tctccagcaa tacggtgttc
    46681 ttcgcctggt cgggcgcgta tccgggtggc ggcctctacc tgcgaaacgc gtcgcaacgg
    46741 gctgtcaact gccttctgga gggcaaccgg aatctgggga cctactggga cgcgggcggc
    46801 ggcgcgggcg tgatcgaagc gggggtgttc cttggcggcg tcgtgcgcgg taacgggtcg
    46861 tacaacaccc acggaggcgg cttcttcctg aacggtgcat gcctcgtctc caacgtcgtc
    46921 ttcgacgcga actggtcaac ggacagtagt gatcggtgcg ggtctggcgc gatcgagttc
    46981 cgcggtggcg gcgctgtcgt ccgtgactgc tggtttgtcc gcaacatcgg gggcgacaac
    47041 gccaacaaca gctacggaac ggtcttctac gggtcctacg acaccggcag cgtgttgcaa
    47101 aattgtttct ttgcaaataa cacgggcgcg tgcgtcctcg gcggctcgat cgagacgaac
    47161 aaggccgact gtgtggcgga ggcctgcacg ttcgtgaaga accatgtgtc gcgcggggcg
    47221 gtctggtgca cggcgctcga ctattcggtt ccggggacgg ttcaaaagat ggccttcacg
    47281 aactgcgtct tctggaagaa cggcgagaac gaggacaagc ccggtattcc ggacaagttc
    47341 aagaacgtct cgacgaaggt atcgtactgc tacgacgaga tcggcacggg cctcacgccg
    47401 ggcgactacc ataacctgac ggcggcgaac ggcgagccgt tctatgcgga tgcggaaaac
    47461 ggcgaatggc gcatcggcag gaaagcgagc gttctggtcg acaacggcct gaagttggac
    47521 tggatgggcg acggagcgaa ggacgggccg ctcgacatgg gcgacggaac ctggagcgaa
    47581 gcgccggcgg cgacgatttc cgtgcgcggc aaaacgatgg acgtcggcgt gatcgtcacg
    47641 cgcaacaatg cgcaaccgcg cattagcccg cagaacggtc tgcccgacat cggttgcgcc
    47701 gaatatcagg cggtgaacgg attcatcttg cgtgtgcact gatcccgggt ggtctcggta
    47761 tcacatcgcc cgcaatggaa atcttcattg cgggaaccaa aggattcatc atgagaagtt
    47821 tcaacttcag tcatgccgtt cttgcgttgt cgacgattct gatcggggcg tccgcgtccg
    47881 cgcttgacat tctcgatgtg ccggtcgcgg cggtctcgaa cagggtctac ctcgccgaca
    47941 tgccggagcg cccctggtgg aacagggcct ggaaacgccg tgcggcgatt ctcgtctcga
    48001 acatgtcgga tgtccgcgag gagcgcgcga tgatcgattt catcttcgac gtcagcgagg
    48061 cggttgaagc gaagtccgtc cgcgtggtga cgccgtgggg gaccgcggtg ccgtgcgtcg
    48121 ccgaaccggt gcgtcagggc gagggcgcgc tgatcacagc acgtagccgg gacgtccccg
    48181 ccctggtcgc gccaaccgcg ctccggctcc tcttcgagac gtcgctccgc gaacgcgaga
    48241 acaggccgtt cttcatctac tggggcaatc ccgacgcgcc gacgcagggg atcgactcgc
    48301 cgatgacgct ctcgtgccgc gaggacgagg tgcgtgtcaa caacggcatc atcgaggtcg
    48361 tcttcgacaa cctccgccgc acggacggcc tcatgaagaa gctgaaggtg aatgcctcgc
    48421 aggcaccgag cgaactcctg tggcgcacga cgggcttcgc ctggcggggc tttgagttct
    48481 ctgccggaag ggccccgaaa ccgtggtcga aggcggtgat caccgccgac aacgccttca
    48541 ggaaaaccgt gaccttcacg aacgaggacg ccgtgctcga cttcacgttt tacgcgggcc
    48601 agccgcgtgt cgactatgtc taccgtctga aggacggtct ccacaacgcg cgcatcgatg
    48661 tttcgtgggc gtgcggcggc gactgtggcc acgatgactt cacctaccgt ggcctttcgg
    48721 gccagctcct cacgttccgc gcggcgctcg atcatgtgac ggactgtatg ccgcagcccg
    48781 aatactaccg cttcccgtgg ctctcggaag gctggtacgc gatcactgac cgtcggaatc
    48841 gcgatgtcgt cggtatggtc ttcgaccgcc gggctctggg cggcgtctcc tacatcgggc
    48901 aggggcagtc gagcggcgag tatgtttcgc tgaacttcaa ccacgcgccg aaggggaaga
    48961 cgcccgtcac gggatcgggc gcgctcgtcg cgacagtcgg cgaggcgcgg acggtcgcca
    49021 ccctctacgc ctggctcacg catccgccga aggtcttcgt cgcgaaagcc gagtcgtacc
    49081 gagagatccc cgtgaaggtt ccggttctgg accgtgactt ctgcgtcaac gtcaacgtcg
    49141 gcggctggcg ttcgtccacg ccgcttcccg gcgacgagtg ggcgccgaac atcatcgcgc
    49201 acatccgcgc acttggcgcg aacacgatcc ttctggggca gttgacggac tacagttgga
    49261 cgtcgctccc gatctcgaag gacctcttcg accgactctg cgcacacaag aaggcgcgca
    49321 acccgtcatg gaaggctccc gaatggaagg gcggttacga cgggcggctt ctgaagcgca
    49381 tctgcgccga ggcccactcg aacggactgg ccgtctgcac atggcacgac gcgttgccgg
    49441 gcttcagtta cggcggcaag ttcgacgccg aggagcaggc cctctgccgc gagatgcagg
    49501 ccctctaccc gatgtgcggc gtcgattcga ccttcaacgc gctcggcggc ggcgaaggcg
    49561 tcgagttccc ggacgacgac ctcaagaagt tcggcagggc ctattggaag tggccggacc
    49621 ccgagccgtt cttcgcctcg cgccgtctga gcggcgaata catgaaggcg ttctacgagt
    49681 attcgcacaa ggtcaatccc ggcacgaagg tgatggtctt caactccgag aacagcgagc
    49741 ttgaacgcga catgtgcatg ggttactacc ccggcgtcat ggacacgctc ttctgtgaat
    49801 tcgtcgcggg catgaatctc tcgaagatca agcacacggc caaacgcctg aggggctatt
    49861 tcgacaaccg tgacggacac acgattcacg cccactacta caacatgaca aaggactacg
    49921 cccaccgcat cggtcagctt gagatgccgt tcatctgcgg gatcaacggg ttctcgcagg
    49981 agtcgatgac gtacgaaaac ttcgaccgcg acctcttcga gatcacggcc gacttcaacc
    50041 gcttcgcgct ctacacgaag ctcgggccga aggtcgcgaa gatggccccg gtgaagaacc
    50101 tcgccgtcct gcgcgacatg gcctgcttcg aggaggactg ccgcaagtac cgtacgatgc
    50161 cgcgcgactg gtggcatgcc tcgcgccacg acgcgcgcgt caatgcgttc gcggagatct
    50221 tgagctacaa ctacgatgtc gtcatcaatc cgcatttcaa ggccggctcg ctcgctcgct
    50281 acaaggccgt ctatctcacc gacgatgagg ttctcccgga agccctcgcg gcagagctcg
    50341 tcgcctacgt gaaggcaggc ggcggcgcgg tgatcgaggg gagtacgggt gaaagactga
    50401 acgttgagga ggtgaaaggc tgggaggacg ggaaggtcat cgaactcggc aagggccgga
    50461 tcgtctggac gaaggaggtt ctcaccgatc cgatgatgaa gggcgatgcg aaggcgatcg
    50521 cgcgtgtgaa ggcgctcgtc gcgtccgtcg gcggcgtcgc tccgttcgag attgcgtcga
    50581 agtctctcga cggcatcctt caggcaggtg aagaggggat gttcctcggc gtttacaaca
    50641 agggcaagac cgaagacagg gggaaggtga cgctttacgt cggcagtccg tcgtatgttc
    50701 tcgacgtgaa gaagggcgtg cgcttcccct tcaccaatgg cttcgagatt gcggttgggc
    50761 cacggcagtg cggcttctac ctgatcggcg gcgacgcgtt cacggcgatt cctgccgcgc
    50821 aggaggctgc gtggatgggg gcggcgctcg cgagcgtccg gccggcgggt cagaagccgc
    50881 cggagaatcc cgatcccgcg ttccggccgt acgtggcggt cgagttcacg tccgtccgcg
    50941 gcgagggcgc gaaggcaaag gcggccggca tcacgcgctc gcgcctggcg ggtatcgaac
    51001 gccggcgctt cgtcacggac gaggcgacgc tctgccgcac actcaaggtc gacgacctcg
    51061 atgtgtggcg ctcgaacaac acggatgtga agacctattc ggcgcaggca tttgccgcag
    51121 cgctcaagac ggcgcattac gtccacttca gcgccaatcc gaaggactgc gacgcggcgt
    51181 tcgcggactg cgccgaggaa ctgaaggctc ttctccggcg cgggggcggt ctcctcttcg
    51241 acaggaacgg cacggggccc gccgcgcgcc ggttcctcgc cgagatcggt gtcttcgacc
    51301 cgtacccgac ggcgaaacgc ggcatcggcg acggtgcacc ggcgtgggac gagacgttcc
    51361 cgacgaacca cccgtactac gcgacgaacg agacaaagcc gcgctatggc gtccagcggc
    51421 tcgaggccga cggcgggcag tattcgctcg ccttcacgaa gtgggatccg caaacgcagc
    51481 ttgcgccgtt ccgcacggcg cgggatccgt cggcggccga actcgtcatt caggacaagg
    51541 ttttgggcgc cggcaaggtc gtcttcaacc agaacagccg ctccttcaac gactggtacg
    51601 agacgaagac ctacggcaac gtgatcctct cgtggttcat cggtatgccg gttgacgagc
    51661 acgcgaagaa ggcgcggttc ttcgtcggcg gcatcggaga gagcgtgaaa tgacgaaaag
    51721 gaaaaggatg atgaagggca gggtgaaaca gttggtgatc gctgccgttc ttgccgccgg
    51781 ttcggttgag gcgaagaccg cgttggatgt gccggtgtcg ggtgtgacga acaagatgta
    51841 tctgtcaagc gcggcgacgc gtccgtggtg gaacacggac tgggcgcgcc gggctccgct
    51901 ccttgtctcg tccatggcgg acatccagtc gggtgccgga tgggtcgact gcgtggtcga
    51961 cttcggcgag acggtgaatc ccgacgagat tcgcctcgtc acgatgtggg agacggaaat
    52021 tccgtgtgtg gcagagaagg ttgatggagg acgggtcagg cttatcttcc agctgccgct
    52081 caggattcgt gaaaacaaac cactgctcgt ctactgggga aatccgaagg cagagaagcc
    52141 gacgttcatc gaaacgatga acgtgacgat ggacggggcg acggcgcggc tggcgaacgg
    52201 gcgcgtggag ctgacgatcg accgtcggca ggaggcaggc aatacgatca aggggtttcg
    52261 tcttgtcggc tcgcagacgg gcgatctcgt gacgctgcgt gcgacgggcg ctccgtggaa
    52321 gggctttacc tttgcgacgg gacgttcgaa cgtcgtctgg aaaacgtgcc gcgtgacagc
    52381 ggacaatccg ttcaagaagc aggtcacgct cgacgctccc gaggcgacgc ttgtctggac
    52441 gatgtatccc ggccagccgc gtgcggacta cgcctatacg ctcaagcggg atccgagcgc
    52501 ccgcattgtg gtcgcctggt gccctggcgg cggcgcggcc tgggacgatt tctactatac
    52561 gggcctcacc ggcgcgcgtc tcacgttccg cggctcgctc gacgaggtga cggactcggg
    52621 cggctttccc cataacgacc atctcgagaa atggatgaag gagggctggt acgcgatctg
    52681 cgaccgcaag aacggcgaca cggcgggcat ggtctacgac cgtgcgtcgc tggcgcgcgt
    52741 ctcttacggc gcggctcatt ataacggtgg cgagcgcgtc agtctcacgt tcgagcatgc
    52801 gtccaagaag ggcgagagcc tccgcggcgc gggttcgggt gcgctcgtcg gcacattcgg
    52861 cggtgccgac caggtggcgg cggaatacgc gcggcttgtg gcgccgccgc aggtgtttct
    52921 tggcaaggcc gaatcgtgcc gtacgattcc catccgtctg cctgaccaca cgaaggactt
    52981 catgggcgag ttcgtgacgg gcatgaaccc tgggaactgg gaacatcttc gtccaggcac
    53041 gaaccccgac tgggccgaca atgcggcgaa ttatctgcgg cgattcggcg cgacggcgat
    53101 tcgtcttaca ggatacggct ggcaggaact gtgcttgact gaagacctct acaaccgctg
    53161 taaaacgatc atccgcgaag atccgaagat cgccgatcac gtcaagaatc gtccgatcgc
    53221 cgactggtcg gtcgaacggt atgcgggcgt gaatctccgc aaggtgtccg aagccgcgca
    53281 ccgcaagggc cttggcgtca gcacctggac ggggaggggg gccgagatct tcggcggtct
    53341 cgaggcggac gtcaccgaga cgccgcttta ccgtcagctc gacttcgacc tgcagaaccg
    53401 ctatccgaag agcggtgcgg gtgtcgtcta caacagccag tgcagtttgg aaggcccgtg
    53461 gatgccgaag gcgctccgtc aggaacgcgg caacgagtac tggcgctggg agaagccgga
    53521 ggacttcttc aatgcgcagg acgacttcat ccgctacatg aaggcgtttt acgcgcagtc
    53581 gaagcgagag aatccgaaga cgcccgtctt cgtattcagc gcggagaacg gcggcctgaa
    53641 ccgcgaactg ccgcttgcgg acctgatcgg cgcgtttgat gcggaatact gcgaacttgt
    53701 gccgggttac cttgcgagcg cccagggcat caagcacagc gtacggcgcc tgcggtcgca
    53761 tttcgacaac aatccgcaca cggccttcca ccatttctat tactacacgt ccgaccacgg
    53821 ctggcgcatc tcgcagttgg aagtgccgtt cgccttcggc gtcaacggct tttcgcacga
    53881 ggtgatgatg cacgatacga tcgacgtcga actgaacgag atcacgggcg acttctaccg
    53941 gtttgcggaa tacacgcgtc ttggcgggaa ggtgtcgaag atggcgcccg tcaagcacct
    54001 tgcggtactg cgcgacgcgc ggctcttccg cgagaacgtc ctgaacaagc gtccgaacga
    54061 gcaggagagt cgcctgcgtg gctttgccgc gctccgtaac tatccgtgcg atatcgtggc
    54121 gaatccgttt ttcacatgcg actcgctcaa gcgctatacg atcgtctacg tgcccgacaa
    54181 ccgtgttttc accgacgaac aggctgcgga actgctcgcc tacgtcgaag cgggcggaaa
    54241 cgccattgtt gaaggtgtga caggcgagaa ggtcaaggga ctggtcgacg gcgagatcac
    54301 ggtgctgggc aagggcaaga tcgtctggtc gaaggaggtg ctgtccgaac agctcgcgaa
    54361 ggacgatgcg tcaaaggttc ttccgcttct ccgcaagctc ggcggcgagg agccgtattc
    54421 cgtcaaggcc ggcggctcgt gcgacggcct cttgcaggcc tcgggtgaag ggtttttcta
    54481 tgcggcctac aacgtcgcca aacacccgag caagggaagg tttgtcatga aggaagaagt
    54541 tcggaagagc ctctttgttc tgaacgtgcg gacaggcggg cgcaagccgt tcgacggttc
    54601 gttcgagttt gaggtcgggg cgcagcagtg cggtttttgg ctgatcggtg acgaggcctt
    54661 cacggctgtt cccgaggcaa aggcgtgtgt atatgacggc gcttcggcgg tttcggcgaa
    54721 gcccgcgccg gtgaagctgc cgcagcacga cgttgagaag gcaaaggcgt tcaaggagct
    54781 tgagatcgtc gaggtgctct cgctcaatcc ggggttcctc ggctatcaga cgggcggcga
    54841 gatcgggcgt atcgaccgtc acgagcggct caagctcaag ctgacacggt tctatccaga
    54901 cgaaaaggcg gttgcggagg tcgtcaagcc tgaggaactc gatacgtgga aacagatcaa
    54961 caagggactc tacaatccgt ttgatgaaac ggcgttcgcg aaggcgctca agacggcaaa
    55021 ggtgctttac ttgcgtggca gcgcagcgca ggatctgccg tttgtccgct gtccgcaagc
    55081 ggtgaaggac ttccttgccg gtggcggcgg catcatcttt gactgtctcg atgcgaacac
    55141 gaacgtgacg gccttcctga aatcggtcgg cgtggaggac ccgatggcgg cgggacggtt
    55201 cgtcaagtcg tgcggacagc gggccgtcaa ggcgccgggg ctcgagcatc cgatcgtgcg
    55261 cgatacgaac gagcggatta acgattttaa ccgcgagggc ttttgccgct atcatctgac
    55321 gggcggcggt tggaagggct ggagcaagga tcagtttgcg cccgtgcgcg catttgagga
    55381 tccggaacag atcgcgacgg tgatcgtgca ggagaacgtc tgcggcaagg gccgcgttgt
    55441 cttctcccgc aactggaacg cctggtggag ttatcggagt tcgatgtgcg acgggctttt
    55501 gtccacgaac atcctctctt ggctgttcga ctacgacgta aacgcatacg agagcgactt
    55561 acaggtcaag aacggcggtc cgggagaggt ggtcggggag cagtagatat ccaggttgaa
    55621 acaaaaatta tgtcagtcga gttccgtaac gtcaggcgct gtccgctgtg gcggcgccgc
    55681 ccaaagccgc cgccgccagt gcaaccgttg caaaacctcc tgcctcacca tcgcgggtcg
    55741 aatgtcgcgg gtcgcggggc gacgatcccg atcccgaccc cgatttggat gaaaccctga
    55801 aacacatgaa acttcatgaa aatcaggaat tccctctggc gttccaaaaa aacccgagcg
    55861 atgccccaaa aaatctgcgt aatctgtgta aatctgtgga taaaaacccg agcagtgcta
    55921 tcccgtgtgt tcgtggttaa ataatcgatc ccgatcccga ccccgacccc gatttggatg
    55981 aaacccagaa acccagaaac cctgaaacca gaaacccaga aacccgaaac ccctgaaacc
    56041 agaaaccctg aaaccctgaa accagaaacc caagagaatc gttttcacca tttcggctca
    56101 acgatttcgc ctaggccacc gtggtaagcc gttgccttga tggcgtgttc ggtcgacggc
    56161 acacccgtga tgtacgaaag ccagttgtca ccgaactgct tcgactcata ccagtcggtg
    56221 aagcagcgct ggttctcgtt gaagatgacg cggcccgtgc cgaggacctt ctcttgaatg
    56281 accagtcccg cgtatttctc gccgtctacc ttggggcgga aaagaacttg ctgcttggcg
    56341 gcatcccaag cgccgaagac gcgtctgtac tggaagtagt tgcaaaggct cctccagagc
    56401 cagaattttg tgccccagac ctcctcttcc ttgccgagcg atttgtggca cgaggtgagg
    56461 taggggtgat cctcggggcc gcaccaggtc gcccaagtat cgccgacgcc ggcttttgcc
    56521 gaaggattcg ggtcaaagac ctcgatttcc ttaaagaact tctgcgccgc aggccccgtc
    56581 tcagagcgcg aaatcagaat ggtcccgctg cgcttcagca acgccttcag ctcctccgcg
    56641 cagtccgcaa agacaaggtc catagccttt gtgtccgtgt cgatgaaatg gataaaccgc
    56701 gcttcggcga gcgccgcctt gcagtccttg acatcatagc tggcgggcgt gaaagcgcgc
    56761 acatcgtatt tcgcctcgac cgaacgcgcg atccggatcg gcttgccctt ctggtttgcg
    56821 cggacgaact cgatcgccac aatgggcttg aagccggtga tgtcgatgac cggaggcaca
    56881 tggggcctcg gacggaacga gcccacccca gcgcccttcc attccgcgac cttcacaacg
    56941 gggaccgctg tgaaggcatc gtcgccaatg aggtagtacc cgcactgccg cggtgcgacc
    57001 gcgatttcaa agacgccgtt ggtcaccgac cggcgtattc cggttttcac gtcgagcacg
    57061 taaaggggag tcgtgcgtac gtgcgcgacg gtgggggaaa gggtcacgat ccctttctct
    57121 tccgccctgc cgtcgttgta cgcgccgagg aacatgccat ccggaccggc ctgcaggatg
    57181 ccgtcaatca tccatgaacc ggtgagtgtg aacggctcca ccccgccgag gtcagcgatg
    57241 gtcttgcgca tctccgcgat ggctttggcg tcacgcttgg cgaggcggtc cgtgcgcaca
    57301 tccttgaacc agaggacctt gcccttaccg agcgtcttta ccttggcatc ctcaagctcg
    57361 ccgaacacct ttttggcgcc gtcgctcagc tcgccttcga aaacggcgtt gccaccctgc
    57421 tcggcatagg cgagaagctc cttcgcttcg ccttccgcaa gaacctcgtc ctccggcacg
    57481 tagacgacgc ggtattgcgc gagatccttc gcggtgaaga acttcggtcc gatcacgtcg
    57541 taggcgaagt tcttgatctc gctgaacgag cggacgcgtc cgtcctgctg ggatcccgcc
    57601 ccatagggct tgtccagacg cttcttgagg acatcggctc ggaacatctt cgggtcgcgg
    57661 tagacaccga cgttcttgac aggtgccatc cgcgcgacct tgtcgccgag acaggtgtac
    57721 tcggcgaaac ggaagaagtc cgccgtgacc tcgctcattt ccagatcctt cgtctcatag
    57781 ctgaggttct cctgcgagaa gccgttgacg ccgcagacga acggccactc gatctggtag
    57841 atgcgctcgg tcgttttcgg gttcatgatg taatagtggt gccagaccgt atggccgacc
    57901 cggttgttga agtgcgagcg catgcgcttc gagacgtgtt tggtgtgcgg gaaaccggca
    57961 tggggcagca tttcgacgac catcgtatcg aagtagccct catcctcgct cataaacttt
    58021 tcgcgcgaat actcgccgtt ctcggaattc cagaggaaga cggggatgtc cggatggagt
    58081 ttcttgaact tggtatagaa gtcccgcatg agcccgtgct ggaagtcgaa cttgtcaaac
    58141 gcctcctgcg gattctccca actccagtag ttggggctgc cgccgttctt cttaacgagg
    58201 tcggacggca gtgccacgcc ctcgccctcg atcgcattgg aatagccgca gtcctgtccg
    58261 cacttgacgc gcaggctcat catgtcgacg ctgaggtcgt tgatctcctt catgaaatgg
    58321 ccattggctt ttgacgacca gccggggacg aatccgcacc aggtatggac gcccataccc
    58381 ttggcatgga tcgccttcgt gaacgcgagg atctcctgtc cccgtccctt catctcttcc
    58441 cagctcggac tcttgcgctt cggcacccag tcttcgaacg cgccgctcgt ctgggcggcg
    58501 atcaggcggt catagagcgc cttgtcctcg atcggcatca tccaccaggg gtaacccatc
    58561 aggcagacgg ccgtcgagcc gtaactgcgc aggcgctcgc agatcctgtc cgcccacgcg
    58621 ggatctttca tcagcggctc ggcggtgccg ctgtccccgc cgtagccgta gccgacattg
    58681 tggtcgaaac agaagtcacg gtcgaggcgc gggatcctat acggcttcgc cactttcgcc
    58741 tgcaccgcgc cgacgctgac gatgacaggc ttcgcgatgc gcttgcagat gcgacggtag
    58801 tcgttgacgt cgccgagtgt ggcgacgacc gctcccgacc ccgcggcctt ctcgccgcgt
    58861 tcgagcttgt gccggagctt aagccccgcc gaaacgccgt agtagctgtc gtagccgaga
    58921 cggcccgcgg ccgtgacgtc gaaggccaga ccggcgatgt cgcccatgcg gcgctcgccg
    58981 atcgcatacc agccctcgcc cagccagtcc tcgaagcgcc cctcgggatt cgtcatgctg
    59041 tccgtcacat ggtcgagccc cgcccgttgg gtgaggatct tcccgttctt tccgcagtag
    59101 aagaagtcgt cgaaggcgct gcccgtgcca aggccccagt tcatgcggat ggacgcctcg
    59161 tcgccgcgat ggatctcgta cgaccaatcg atgcgcggct gttcggcata gagcgtgaac
    59221 gtaagggtcg aatccgcgca ttcgaagcgg attgacttca ccagggcatt gtcggccgtc
    59281 accgtgccct tgtcccagct attcgtggcg tttggatgga acacgaagcc ggccttcgca
    59341 tagccggtcg cgcggtcgaa gaacaccgtg cggcagttcg agccgaggat gcgcagcgaa
    59401 cggatgaggc cctccgtcac gtgtctgcgg tcgaagacca catccaggac gccgttgcgg
    59461 attcggaacg tgtgctcgtc cgtctccagc gtcacgtctg cggagatctt ctccgcctgt
    59521 gccgcagggt tgccgaaata cacaagaaac ggcttgttct catgctgccg gagcgtcgtc
    59581 ttgaagagga gccgcacctt cgacccctcg gctttctcgc acacgcacgg cacgaccgtc
    59641 tcccacggtg tcaccacgcg cacctccttc ggctccacct tctcgccgag gtcaacaatg
    59701 gcgtcgacaa tgactttgtc atccttttcg tcggcctgcc ccgacaccaa cagcggcaca
    59761 cgtttctgcc aggcatcatt ccaccaaggg cgctccgtca tcgatgtcag atatgccttg
    59821 ttcgtgactg tcgacaccgg ccagtccagc agggcattcc atccctgagc aaatacggag
    59881 tagggtacgc tgcaaacgga gatgaggacg gctaacagaa tggactttgg tttcatcgtt
    59941 gagtgtttcc tttcctgcgc cttaccttcc cgcgtccagc gcgaggacga agcccggcgt
    60001 gggcagatgg tcgatattct tgaacgggct cagatgccac cagttgaggc gcacaccgcc
    60061 cggattgctg atctttgagc agtcgagata gaccttgttg tcctcgacgt acaggttgtc
    60121 gatgcgcgtc cagttcgtct tgcccgccga gcggacgtag aacttgcgga agagcgtgtc
    60181 gctgacgatg agcgactggc cctgcagagc gggcgtctcg aagtcgagga ccatccagga
    60241 ctcctccatc gtcaccttgc cggggagcgg gtgtatcccg tcgcgcttcg cgggatcggc
    60301 cgcgtgctta agcgcgagcg cgatgcggcg catgccctcc ggcgcgaaac gtttccaact
    60361 catcgcaagc cgcgcccccc cgtcgcgcgt cgtgatgacg gccgcgcgtc cggcgaaggc
    60421 gtccctcagt cgctggtagt tctcgaaatc gccgccctct tcgaccgcca cgacggcctt
    60481 cgcgcggacg gagtcgagaa gcgcgttcat cgccgcgaga tgctccgcgt tgacaatcga
    60541 atggtctttc atcttcccgg tgtcgaagcc cggcaccaga atgatgcccg catagctctt
    60601 cggcgcacag gccttcagcg cgacggcatt cgtgacctcg acgacatcga ccgcgacggc
    60661 cgtgccgttc ttgggcgaga aacgcgccac gagttccttc gcgatgggtg aatccccgcc
    60721 tttctccatc aggccgccgt gaccgaccac gagatagccg tcgtccgcaa acgcgaaagc
    60781 ggccgccagt gccactgccg ccaaaacatt tcttttcccc ttcacaatcc cccgacccct
    60841 tctccccaat ccctcagata tgaaccgtca cggcctgcgc gaaacccgtc acgacgacgg
    60901 cgccggacgg atagaccgcc gcctcggcgt aggcgttgtt ctcgcgctgc gggcccgtga
    60961 cgatcgccct gagcgtatag tacccgacgc cgcgttcctt catgtagccg ccgatgtggt
    61021 catgtccctg aaagaccgcg gcgacctttt gcgagtccgc gaggatcttc tgcacctccg
    61081 cgccgttggc gacgcggtgg agcggacgcg cgcccgagtc gaggcgctga tggatgcaga
    61141 caacgacgtg gcccttcgcg gcggcgagcg tctccttaag ccaggcaatc tgggtcggcg
    61201 ggatgagcga ctcgcgccag tcccagttga tcttcgagcc cgtgtagtcc tcgcccttca
    61261 ggttgaagtt gccgtccaag atgatgaacg tgacgccgtt ttgatcaaac gcgtagtacg
    61321 tcttctccgg cgggatgccc gggttcggac agagcgcagt gaattcggac ttgtcgagca
    61381 tgtcgttgtc gtggttcccg aagacgtggt aacgcggacc gttgaactgc gcgaagacag
    61441 cctcggcctc cttcgtgagc ttgatcacgg tctccttgtc gcggtcatcc tcgcccggtt
    61501 tcggatcctt cttcttggca atgtccttga agtcgccgag ttcgatcacg agatcgctcc
    61561 ggcgctcgtt gaagaccttt atcgcctcct cgaggcgcgg caaggcacga tcataaaagc
    61621 gaccgttatc gaaccacgcc tcgggcagat aagcgtgatg gatgtccgtc acaagcccga
    61681 cacggacgag cggcttttca tcttccggca cctttcccca gatcggatac gcgacgagcg
    61741 cccccgcgct cgcgagaaag gcgcgcctgt tcatgttttt tgccgcagtc atcgatccct
    61801 ttcttttctt attcccctgc gaaggatgga ttttaattta ccattaccgt tgtgccggac
    61861 ggcatgtatt cgcatgcgcc gatttccggc gcaaccccga tgatgcggct cttgacattg
    61921 ttcggcacga tcttgacgcc ccattcgccg ctcttcataa tcgtgaacgt tccgtcaccc
    61981 atgtcgctcg ctccagcctc acgcatccgc ggcagagcga aaccgcgttc cgttgccacc
    62041 gaacttccgg ttgggatgaa ggtcccttcc ctgaacgtga tcgtgccggt aacgttgtgg
    62101 cagccatccg gcagatgcgt tcccttgccc tcatccgccg cgcagtaggc gatgttgccg
    62161 accgtgccgt tgaccttcag gtaatcgttg aaagcctgct tgccgttgtt ggcggtgacg
    62221 agacagttgg tgatcgtgat ctcgtcggga tagctcgtgc cgccgctgcc cgcattgacg
    62281 ccgatgccca cgtcgctgca ggtgttgccc gcgaccgtgc agttctcgac gcgcacccac
    62341 gggtcggtga catccgacca ggacgttttt gctgtgatcg aaacgccgcg tttgcaggta
    62401 ttgcccgcga tgagcgtgtc gcgtaggatc aaccacctgg cataagtctg gccacgcaat
    62461 gcaaccgggt tgttgccgca gtcgttgtcc acaaaccagc accgctcgac cgtgccggtg
    62521 ccgtcggccc cggtggtgtg cgcaaacgcc gcttttttgc cggtgttgtt ccgtatgacc
    62581 gaattgcgca ggatcgtgtt tttgccggtc atctgcaggg gtgtgccggt attgttctcg
    62641 atcaggcagc cgtccatgat gccgaaaccg agataaacgg atgtcccgct gcaattgatg
    62701 aagcggcagc cgcgaaacgt gccgagccac tgctccgagc cgctggtcgc gccgaagttt
    62761 ccgccgtaac cggcggtgtt gatgcaattc gataccgtgc agtcgtagac gttgccgccg
    62821 taactgcagc agatcccgcc gccgttacca ttcgccgtgc agttggcgat gaaggagttg
    62881 gtgacgacat tcggtttcat attgcagatg ccgccgcctt cgcagctcct cgttccgctg
    62941 gtaccgcagt caacgacaac gcaacggtcc accagtccat tctgattctc ggccaagtag
    63001 atgccgcctc cgcggcggtt cccgttgacg tccttgtccg cgataagcca gcaattcgag
    63061 accgtacagt cgcggatcgt cccgccgtac atataaactc ctccgccggt aatctcgcct
    63121 gtgagcgaag ccgcggtatt gtgattggtg cagttgtaga caaacgaatg cgtgaacagc
    63181 gaattattgc cgttgatctg cacggcgcca tgttgattgc cttgtgtctt gccgggcggg
    63241 atacggtcgt ccggttcggt gatgcagcag cagacgatca tgttggacgc catcgcgccg
    63301 ttatataccg tcacgccgcc gccgcccatg tgagatgtgg tcaattcgta gccgcgccaa
    63361 tagtaggtcc aaagtccatt cgtcaccgtg aagccgcaaa cgctctgccg gtagagggtc
    63421 ccatcgtcgg acattttcca accgcgcagg tcgaagccga actggcggtt gatcgatccg
    63481 tcgatcaccg tttcgcgcgg gttgtcgttg tcgacgtcga cgccgttcac gcgcttctgc
    63541 gcacgcacgt caacgaaata agaggtgcta tttacataat tgctgcccgg atagacgcca
    63601 ggctggactg aaatggtcgt caaaacattc ttctgttcct taaccgccac atccattgcc
    63661 cgttggatcg tcgcgtacgg cgcgtcccag gagccgtcgc cggtcacgtc attcccgccg
    63721 tccgccgcgg ttttgacgta aagtttcggc acctgcgccc atgcgcttac acacgcgaac
    63781 accaaccaac ccgccgtgat gagtcttgac gttttcatat cgccccctta tgaacgcacc
    63841 aatatcccat agggtgaacc ggcaacacac cggttcctgt gatcagcacc tattatacgg
    63901 tgagggtgaa aacagtcaat cctcccgggg gacccactgg acgggctgcc gcagtacggt
    63961 gtacactgtc ttcgttggtt tcggaggcgc gaacccaagc aattggggga ttcgtttttt
    64021 catgatgcct gtgggctaca ggcaaacgcc cctgaagccg acacgttttg agaattttta
    64081 gggttgactt ttaccccccc cgcacaatgt gcgccaacct tggggatcgg tggggtgccg
    64141 gtctatccta tgggacggta gtgaaaatga gtactgcatt acgatacgat gtgattgccc
    64201 ttaggatatt gtctggaaga gtgcttggag tcctgctcgg agtttttgct ctaactgaaa
    64261 cgctatgggc ggatttctcc ccggatcggg cggtgatggg cgagaagtac cggttgaatt
    64321 ggaacgcggc ggttgatgcc gagatcgatg cgcggattga gaaacaccga aaggccgact
    64381 gcgtggtggc gctggacggc gttccggacg gaaccgttgt ttgcgttgaa cagttgacgc
    64441 acgatttcaa gttcggctca catattttta atttcgatca actcggacga gatgattgga
    64501 atgacatgta caagtcgacg tttacgaatc tctggaatgc cgcgacggtt gcgttttatt
    64561 ggaagaatta cgagccagtc gaaggggcgg ttcgttttgc ggacggtccg cacgacggag
    64621 cagcgttctg gaattccaaa gcaacccttt cgccaaagga gaagaggttg gctttcaatg
    64681 aatggaggcg gccggcccca gatccgattg tgaatttctg cctgcgacat ggactgtctg
    64741 tccacggaca cgcgatgatt tacggggctt atacgccggg ttgggccacg aacattgtcg
    64801 acgcgtcgcg tcttgacgca gtgtacgagc atcggatccg taccttgtgc aaacactacg
    64861 gcgaccgtat cgaccagtgg gatgttgtaa acgagtccgt gagagccaac tcgatgccgg
    64921 aatcgccgga tgacgagggg aaatggagat tccgtatgcc aaagtgctac acgtttaagt
    64981 cctttaagac tgccgaagaa caccttccca ggcgggtacg gctcgcaatc aacgacgcgg
    65041 gggttatggt taagccgtat gtgccgttta tccgtcattt gctcggtaag ggagcgcgca
    65101 tcgacatcat cggcgtgcag atgcatatct tcagtcgaga tgacgtcctt aaggtcgcgg
    65161 acggactcga gtgtatgcca aatctgacat cctgggatcc agtcgaccag ctcgcgacgc
    65221 tgaccaagct ttcacgcctc ggccgtccga ttcacatctc cgaaatcacc atcccggcgc
    65281 cggatgacac gccggaggga tggcggattc aggctcaact catgtatgac aactatcggc
    65341 tttggttctc gtggccggat gtttgtcgaa ttacctattg gaatcttgtt gattattcct
    65401 atgacaagga atcgctcgcc tccgggctct acacgaaaga tctcaggcga aagccgtcgt
    65461 acgaagcgct tgatcggctg atcaatcgcg agtggaaaac tcggatgtcg attgtggtaa
    65521 acaacggccg catcgcgttt cgaggctttc gcggaatgta cctcctgtcc tggatggacg
    65581 cagagggcac gatgcgttcg aagcggatta acctaaaagg agcagttgaa cgggtagaat
    65641 tctagcaaag ccccgcaaac attgggtttt gacgtattga gatgcgggcg gagcccgcaa
    65701 tctcaaaggc tgtaagactg tgaggcggtg agactgtgag gcggttgtgt tctgtggtga
    65761 aacaatcgct cccgatcccg atcccgatcc cgaccccgat ttggatgaaa ccctgaaaca
    65821 catgaaactt cat
//
LOCUS       DAOSLE010000002        49464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_820_length_49464_cov_6.988949, whole genome shotgun sequence.
ACCESSION   DAOSLE010000002 DAOSLE010000000
VERSION     DAOSLE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 49464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49464
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_49464_cov_6.988949"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PK576_00145"
     CDS             complement(<1..196)
                     /locus_tag="PK576_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOE60031.1"
                     /translation="MIIAIASGKGGTGKTTVAANLARVHGAVTLLDCDVEEPNVHLFL
                     KGTPDGEPQEVSILVPEVDEA"
     gene            complement(193..1071)
                     /locus_tag="PK576_00150"
     CDS             complement(193..1071)
                     /locus_tag="PK576_00150"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOE60032.1"
                     /translation="MNTSLDCLPCFFRQTLDAVRRVSDDTAFHEKVVRQAAEWVRNVD
                     LSESPPVMARQLYRYLRELTGVADPYAEAKARDNEMALRLLPGLRERLAASDDPFTLA
                     VRLAIVGNLIDLGAKSDLTTAEVESAIRDVEKEPFSGDVAALRQRAGKARTILYLADN
                     AGEIVLDRLLIEALGPERVTVAVRGAPIINDATMEDARAAGLHELVEVISNGSDVPGT
                     LLEECAPDFRKHFDAADLVISKGQGNFETLSNAPRDICFLFKVKCPVIANHVGLALGT
                     QALVWLQGATKAKKKQ"
     gene            complement(1077..1439)
                     /locus_tag="PK576_00155"
     CDS             complement(1077..1439)
                     /locus_tag="PK576_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007463787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE60033.1"
                     /translation="MKIAFTTSGSDLNAPLDMRFGRAAKFLVYDTATEAFIIIDNEQN
                     LNAAQGAGIQAAQNVAAAGAQAVVSGHCGPKAFQVLNTAGIKVYTSNAATVAEALKLY
                     RDGSLTELKSADVGGHWA"
     gene            complement(1436..1678)
                     /locus_tag="PK576_00160"
     CDS             complement(1436..1678)
                     /locus_tag="PK576_00160"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 family protein"
                     /protein_id="HOE60034.1"
                     /translation="MPGGNRMGPLGMGPMTGRGAGYCAGGGAAGNIMAGGRFCGFGRG
                     RGNWGGGGRRCRNMFFATGRPGWARFEGAPAGKEES"
     gene            complement(1816..2244)
                     /locus_tag="PK576_00165"
     CDS             complement(1816..2244)
                     /locus_tag="PK576_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE60035.1"
                     /translation="MKTAYSIYGTRIAPVFDTARDILIVESRGQEICGESLHKLEGRT
                     PAEDIAWLQSQGVKRLVCGAVSRPLHEMIIAAGIQTEAFVTGEVRQVISAALSGLLNS
                     AVFAMPGCCGRRRMRRACAGGGRGAGRGLGRGRGREQCIS"
     gene            complement(2622..3983)
                     /locus_tag="PK576_00170"
     CDS             complement(2622..3983)
                     /locus_tag="PK576_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE60036.1"
                     /translation="MGRSQIRQTDGDMGTRAILESISYAVFCFEKAWRVTSFNRAAET
                     ITGVPRKEAIGRRCADVFRSNMCGPECALQTTLKTGRPVIGRSGYIINAEGDRIPISI
                     STAVLKDHAGRVIGGAEVFRDLSEVDALRRELEGRFQVGDLVSRSPVMQRLFEVLPAI
                     AASPSTVLVQGETGTGKELVARTLHTLSPRAQAPFVAVNCGALPDTLLESELFGYKAG
                     AFTGATRNKPGRFALAKGGTLFLDEIGEISPALQVRLLRVLQERVYEPLGATRSEKAD
                     VRVVVATNRDLQQLCRDGAFREDLYYRINVVRVELPPLRKRKEDIPMLVDEFIRRFNR
                     LQSKRVTGISAKALSLLMAHDWPGNVRELENVIERAFILCGAENIGLQHLPENLTRAK
                     MTRTQTAASGHIKDTRAIAEAQAIRLALERSNGNRQAAARALGIHKTTLFRKIKALGL
                     EGI"
     gene            complement(4012..4749)
                     /locus_tag="PK576_00175"
     CDS             complement(4012..4749)
                     /locus_tag="PK576_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOE60037.1"
                     /translation="MNSVVKISAAAVILALGMILSSSLLSKLFVRIRHEQTITVKGYA
                     EKDITSDVGKFTATCTVRGSSLKESYDKLRESRNAVMAYLKRSGFGEAELVAGTIQTE
                     KIPKRDAQGKETNETEYFDATQTFTVTSGNVRLIRDVSNGITELIKDGIEIRAGSPEF
                     YISDLKDTKLALLAEATSDGYRRAVALAANSKGRVGSLISAQQGVFQITERHSTDTSG
                     YGAYDTSTIEKTAKAVVTLGYAIEPEK"
     gene            complement(4761..6212)
                     /gene="lysS"
                     /locus_tag="PK576_00180"
     CDS             complement(4761..6212)
                     /gene="lysS"
                     /locus_tag="PK576_00180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOE60038.1"
                     /translation="MSEEQPQSGNEYRAQRIANLRSLEERGFAPYGQAFERTRLSEIR
                     STFTEDRVVRAAGRLMTIRRMGKASFATFSDGTGNFQIFVKKDRLTPEQFDAFAYLDL
                     GDFIGVEGRLFTTRTGEPTLEVHTWVLLGKAILQPPEKFHGLQDVEERYRKRYLDLMM
                     NPESRARFDKRSAILAEIRAFLHARGFIEVETPIMQPQAGGAAAKPFVTHHNALDTEM
                     VMRIAPELYLKRLIVGGYDRIFELGKDFRNEGIDRTHNPEFTVLEVYEAYGDRSSMKS
                     LIEGLMPHLCEKVLGTLQVAYGEAKEMLDFTPPYREIAYRDAVREVMGPDWFDLPVDE
                     ARARAEAKGVDIDPSWNHLMVTHEVYGKVVEKMLRQPTFVTRIPRQFVPLAKTCADDP
                     ELADVFEFVVAGKELCPGYTEQNDPMAQRAALADQAGDNPEKIDEDFITALEHGMPPT
                     GGLGMGVDRLVMMLTGTEVIRDVILFPQMKKQG"
     gene            complement(6276..8687)
                     /locus_tag="PK576_00185"
     CDS             complement(6276..8687)
                     /locus_tag="PK576_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60039.1"
                     /translation="MRKLRSFSILATAAVFGAVAMVAQTLLLRRFLWRFAATESGVAL
                     FLACWLGWTGLGAAVAATRMGRRITGILADRLWLPLSAGIALYFVQYALIGNLRAWLG
                     VPDYQAFPLARLAAGCFVANFPFCFVSGFVLPAVAVRLARDGSPVSRAFAWEALGAAL
                     GGLAVTGLLALGITPDPRDLAEWRRAFPDGTAPSGRFETGGGTTFYGKHRGTFYALSS
                     GGVREVIPETDYAMGWAALLLSQRPYARTVLLLGEVSLATGLALESLRPDLEIVWCPA
                     DPGYGVGLLKSVKAAGMATRIRAAGVTPRRYLADPASPLFDAVLVWPPAATSLAGAVW
                     RETAFLCAVRQVTRRTGVALFGLDCTSAVVTHEQGALLERFVRRIRQVWPESGALAAG
                     AGGWWVAAQVPGLAYGAEDAVRRFALLKSAHYPAEAVRLLYDPARAEAWRTRWPAWLA
                     TDPPLAPPEMSAPETTLAYGLADALSRTYPETAAGSVFLRLLRPEGVRLTAYLLLILW
                     TVPVVVGRAQTAPRRMVAAWLAACGALGLTASLTVLSRLEMRFGSLYLLAGAGSCLYL
                     AGLFCGNRLAEGGMRAMPERPVVTAALPSALTVLLAGCAFAMSAGAERAATAYVTLLL
                     CFPIGVAAGGALPVAWACCGEGPENRAAVFVLADALGAAAAGMFFLVLVPLAGLERTI
                     GTFAALALGFAVCLAFGGSRAARLSAGTALMLACAVCVAQWRELAQARPEEVPGGVGG
                     AASVAVPAARGVRMKEGEGPRSSKPAADACLPGIPRRIDSERVLRQMEAGRLATNEAA
                     YWERE"
     gene            complement(8799..10202)
                     /locus_tag="PK576_00190"
     CDS             complement(8799..10202)
                     /locus_tag="PK576_00190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE60040.1"
                     /translation="MMKLTFFGAAQTTTGSMHLVEANGKRILLDCGLFQGHRKEAFEK
                     NRNMPIDPRTLDAVVLSHAHIDHSGNLPTLARHGFRGKVYCTPATRDLCDIMLRDSAY
                     LQQRDLEYVNKKRAQQGKVLFEPLYEQEDVDHILSRFEPEPLKRLVDLGDGISLLLHN
                     AGHILGSALVQLDVKTRGGTSRRLLFTGDLGQPDRPILRDYDFPQGADTILIESTYAD
                     RDHPAREDVSGRLKGFVEDIHQQKARLIIPAFSVGRTQHLLYELNRLIEQRRMPLTPV
                     YVDSPLSLKATQVYARHRECYDEEASDLLRQGIDPLRFPGLQFVETPDQSKALNDRPG
                     PMIIIAASGMCEGGRILHHLRQGVGDPRNIILIVGFQAENTLGRRIVERRSPLKILGD
                     EFELNARVHTINALSAHADRTALMRWFDAVRGKRLKRVFAVHGEPEKVQAMIELVRAR
                     GVENVVAPQPGQKFEDV"
     gene            complement(10256..10453)
                     /locus_tag="PK576_00195"
     CDS             complement(10256..10453)
                     /locus_tag="PK576_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60041.1"
                     /translation="MRDAGRLVRWGRGMTAVRREEHEDFLGAWIEEFCGMGVPPMKHG
                     QDARAIPAFGVPVILALRWIE"
     gene            complement(10543..11001)
                     /locus_tag="PK576_00200"
     CDS             complement(10543..11001)
                     /locus_tag="PK576_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60042.1"
                     /translation="MATSKRSVKRTSVKPAGKKTAATAARAAAKPEPRKAAPQPVSAA
                     GVRPPVAVERPAAPSTGAVAGPTARTCPLVLDGETSAAAFTPRACSACDEFDCRFNVT
                     EEGSGALRSRLFAVEEEEEDGGEDVLYGADFDEEGDGGFGGADEEDDDLF"
     gene            complement(11045..14251)
                     /gene="ileS"
                     /locus_tag="PK576_00205"
     CDS             complement(11045..14251)
                     /gene="ileS"
                     /locus_tag="PK576_00205"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOE60043.1"
                     /translation="MFEPVSKQTDFPGMERDMLAFWESHDTFRKSVEQRRGQREYVFY
                     DGPPFATGLPHYGHLLAGTIKDIVPRYQTMRGHHVERRFGWDCHGLPIEALAQEALGV
                     SGAQAIREFGVAAFNEQCRSMVLTYVEEWRKTVTRMGRWVDFDNDYKTMDPGFMESIW
                     WVFKQLWDQGRIYKSYRIMPYSWKLSTPLSNFEAGNNYKDVQDPAVTVRVRVRSGAEP
                     AWGTTYLLIWTTTPWTLPENLAICVGPEIDYVVVRDCEDETVFVLAEARLPVYYKDAG
                     AYEVLARLKGEALRDWTYEPIFPHFAEYRDAGAFRVLTDSFVSTEDGTGLVHMAPAYG
                     EDDFRVCQQAGLSVIADPLDAACCFTEAVPECQGRFCKDCDKDLIQWLKREGKLVHQT
                     TVTHSYPFCDRTDTPLIYRAIEAWYVRVEDLHERLTRNNATVRWMPEYVGEKRFGNWL
                     NEARDWNISRNRFWGSCIPVWINENDPADQICVGSVAELEQLSGERVTDLHKHFVDRV
                     VIRRDGKVYRRTPEVLDCWFESGAMPYAQQHYPFGDLGVRSEDLGVEDLGVEEKRGKG
                     GQDISTFFPADFIAEGLDQTRGWFYTLMVLGTCLFDQSPFKNVIVNGLVLAEDGKKMS
                     KRLKNYPDPSHILDAYGADALRLYMIYSPVVRAENLRFSETGVKQILRELLIPWWNAY
                     SFFVTYANVDRFDDAEVVRPESPNVLDRWIVSSLETLIRDVTVAMDGYDLQRSVRPFV
                     TFIEDLTNWYIRRSRRRFWKSQNDEDKTHAYRTLRYVLVQLCQVAAPFTPFISEAIYR
                     NLRGASMPESVHLCDFPQAYAAGRDEALESAMARVQQVVRLGRQLRTENDLKIRQPLA
                     VLHVVSGNAGLRAILQGYEDLITDELNIKTVAYGEDETVMADVSVKADFRQLGPRVGP
                     RMKAVAAAIAALSSAEAAALAAGREVSIAVGGESMTLCAGDVVVRRTPKVGMVVASED
                     DVIVGIETALTPELIREGLAREWVSRVQNLRKEAGYEVTQRIRVTVACGAEVREAIEQ
                     YADYVKAETLCEGIRFDEVHGPVIELNGHAVGLHVEKA"
     gene            complement(15012..15749)
                     /locus_tag="PK576_00210"
     CDS             complement(15012..15749)
                     /locus_tag="PK576_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE60044.1"
                     /translation="MRLTVVIPVYREYRRLKGTLEQVAAFIRDTTEVKVDAILVDDGS
                     PDRCSDVVREFIAASGSDRIRLLGYKVNRGKGYAVRTGVLAAEGDLILMSDADLSTPL
                     QEWVRLKEAVDAGAAIACGSRAVRGAHILTPPPLHRRVLSRVFNLLVRLAGVHDMRDT
                     QCGFKLFRRDVAREVFSRSRINRFAFDVEIIAMARDLGYRVVEVPVIWNYGGHSTVRV
                     FSSGGRMLLDVLWLALRRAVAGPARRV"
     gene            complement(15828..18455)
                     /locus_tag="PK576_00215"
     CDS             complement(15828..18455)
                     /locus_tag="PK576_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE60045.1"
                     /translation="MITRGMRRAAAALAVAAIPVSPVWADGCGASGSATDAAARFILQ
                     LGVLVFAARLGGALFERGRLPMVFGEVAAGVVVGPYLLGGVALPFFPHGLMGDAEAFL
                     SVWGGGGQGLMAVILAVFFFLAGLDTDLRQMRRLSASGVLAGVCGFLFAFGAGLALLF
                     RAAPTLLGPAQPWHTSALLLCAVPISVTSVGFLARLLAVRKRLESPVGTLSLTAAMAG
                     NVLGLIVFTVVAGVSGAGGGVNELSGARIAAVAWRAGVGLGAVLLLGIPLARRMNALA
                     VREKNYGQPFAVSAAGLLIAGGLIGACGLPVVSGAYMMGVAFSAMDVRHEIRERLDFV
                     SLTLMPACFASLGMRLNPNLLANETVLGTSVLLAVAVLGANLIGCAAGASLTRSGVAT
                     SLRVGILALPRAELSVALLAAVGGMIPLPQSLLFCAVFLILCSCVALPFLSDRVLDQG
                     DGCGGSGPEPARLVFRFPSRQAAQLMANRIVELFEDEGFYAQVLNRHHMLYRISRETQ
                     VIHLQSHDGEITFECGERERALVNVVMLEALSDIEGNLCALRRPLDDVLLRKNIQVPE
                     ASAAGSAGVLRNHLFPETCRPRLLASTPAGAVAELVNLLCESGLVTDRDRALQAVLDR
                     EQNLSTGLEHGLALPHARTDAVTRLVCAVGLKKEGLAYDTVDGRPARIVALVLAPEQA
                     ASPQLQVIAHLCRLLDSHGRAALLACDTPDDMYAVLTGGRAGAAFCARQTILARCLRW
                     QSIALDFDVEDRQQGLALLLALCARSGAVNALPEAMADIRRGVETPPVRIGEEVAVFP
                     VRTAHVDRMVAALGIASATSGSGTSPRVWVMVLYPESEATVYAEVEAELRQRLTGQTL
                     DGLLAAKTAHEAFALLTGG"
     gene            complement(18474..19136)
                     /gene="larB"
                     /locus_tag="PK576_00220"
     CDS             complement(18474..19136)
                     /gene="larB"
                     /locus_tag="PK576_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOE60046.1"
                     /translation="MTEDLHFAKPDFDRQRRLGMPEVVFGSGKTAVQIAAIMRALNDA
                     GQNAFVTRVTPEQAAAVQTEIPDAVYREEAHILTRDVAPLPQPEGKVAVLCAGTSDLL
                     VAEEAALTAERLGARIERVYDVGVAGLHRLLSRIDRFEDARAIVVVAGMEGALPSVVG
                     GLTDRPVIAVPTSVGYGASFQGLAALLGMLNSCAAGVTVVNIDNGFGAGVAAARINRQ
                     GA"
     gene            complement(19140..20909)
                     /locus_tag="PK576_00225"
     CDS             complement(19140..20909)
                     /locus_tag="PK576_00225"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOE60047.1"
                     /translation="MSNTSSLDVIASLCKRRGFIFHSSEVYGGINGFWDYGPLGCEMK
                     RNIKEDWWKTTVRNREDVAGLDATIIMHPAIWKASGHLDTFSDPMCTCRACKKLMRAD
                     QVWDILAEGAIGQSLKALLEGGADAAQVAGWCKGKAKGLAPNLAAVLDADTLTAAYET
                     LKADPAPYAAVRALYTLLANAKAPQPAVVPCPYCGGELTEPRPFNLMFRTVVGPVDDS
                     ANIAYLRPETAQAIFAQFQNVLATSRQQVPFGIAQMGKSFRNEVTPRNYTFRSREFEQ
                     MELEFFIRPDEAVELICGHVTTLDDHPDLSVPQDSWGWEVWHKYWVEQRKAWLEACGL
                     PKTSFVEYWQKPEELAHYARACVDIEYRFPFGVQELEGIAARSDFDLSQHQKHSGKSM
                     EVFDEPLKLAADKLDDAAKLAFTDKVVAEWRAHGKDESDARAFVSKLFLGKYVPHVIE
                     PSAGVDRMMLALLCNAYEEQKLTDDKGKEDTRIVMHFSPRIAPVKVAVFPLVKNKPDL
                     YNKARGIFEDLRRRYSAFWDEGGAIGRRYRRQDEIGTPWCVTIDFQTIEDGTVTVRDR
                     DTMLQERLTIEQVKAKLDDALLV"
     gene            21373..22155
                     /locus_tag="PK576_00230"
     CDS             21373..22155
                     /locus_tag="PK576_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60048.1"
                     /translation="MMRITRYVLCAGILSAACCLAAEEGWTPLFNGKDLDGWIPKIKG
                     YPAGENFANTFRVEDGMIKVRYDGYDGAFKNRFGHLFYKTPFSHYILRVEYRFVGEQL
                     PDGAGWAKRNSGVMIHGQTPESMGVNQSFPVSIEVQFLGGLGSGNRTTANLCTPGTNV
                     EKDGKLYTPHGLSAKSKTYHGDQWVTVEIEARGSETILHRIEGETVLQYDKPQYDPKD
                     GDARRLIEANGGKLLIEGGTISLQSESHPIDFRKIEIKVLKP"
     gene            complement(22136..24979)
                     /locus_tag="PK576_00235"
     CDS             complement(22136..24979)
                     /locus_tag="PK576_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOE60049.1"
                     /translation="MLNFRTVWVTRHPGRPPVFDLFPVTDQRDGGETVYVTQDPAAFE
                     GVLPQEGAWAVDVLVLYAFAFPGTPAEQVTTQIRQGPHTGEAIGVLWQAVAARLNALP
                     LWTLESIEGILRNLDEHGLARVFSHFAACVRQAGVGCGPWIETFAPEARRGDRRALPE
                     HADCSPLDVAAMAAHLLPGGAFARLMPGYEPRAGQVEMLHAVTRAFNEGRHLLVEAGT
                     GVGKSLAYLIPAAAWALLNDVPVVVSTNTRNLQAQLIENDLPRVREAVCPGGRAALRV
                     AVVKGRSNYFCLRRLAILLEQSQYELDRPEWRHFAYVLAWAAQTPDGDLDTFAGPGRV
                     DAEFLAKLASTAEECPGRACRYSRRCFLQRARARAAAAHVVIANHALVFAESRAPGTA
                     LPPHAQIVFDEAHNLEEAATRHLSTEITQTRLTQLLRRLSRGRGRRAGGMLEVLRNHL
                     EKGAVTADPEYAQALRRQIREIRNVLEEVQAAARQLFESLHGLIQPEPGPVRFRCVPC
                     DGPDATVWPTGESALAAGNAGQPACGGVFPSGFRREVFRDKRFTPCPDAWDEDTVQMH
                     RIGLKNAIAEASGLLLALGEALRQATEGELALYDDQAANVEGAAASLRTYAMDLDFVL
                     AAYDAEHVFWAEPPSRSAGTAHLYAAPLRIGEALAETLYRRKATVVFCSATLRVGNSF
                     GYISRRLGIDRLEPDRLLTCVAASPFDYLTQCAALAPVFLPEPTGGTGGTYAEQLSGL
                     MLDVFTRTRGRAMGLFTSYEMMNRVARLLEEPLQEAGIRLLVHGTNGSRDQITRIFRT
                     GGNCVLLGTHSFWEGVDVAGEALSCVVMARLPFAAVTDPIVEARCEQIEDAGGCAFRE
                     FSLPQAVIRFRQGFGRLIRIRADRGVVIIADPRVVTKSYGATFRKSLPCPLLRVETRE
                     ELLRRVEALFAESAMAQESARRHGAGDTTASAP"
     gene            complement(25004..25873)
                     /locus_tag="PK576_00240"
     CDS             complement(25004..25873)
                     /locus_tag="PK576_00240"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE60050.1"
                     /translation="MSRAGVLSFVVSKSEAGQSVQVFLAAKLSRSRRAVKALLDERVV
                     WVNRKCVWMAHHTLRQGDTVELPAAVLRKGARDANPVAVPKIRLLIEDAEYAVVDKPS
                     GLLTVGAGSVEERLRAQTGIAELKAVHRLDRDTTGCLLCAKTPEAFDAAVELFKTRRV
                     TKQYHTIVSGRYERHASTVDQELDGERAVSHIQRIMLSRDASFLKVRIETGRTHQIRR
                     HLAAIRHPVLGDRKYGVKVARDPRLLSVPRLMLHASGLGLPHPMRPGKTLQAHSPLPA
                     DFRHCLKLFDMGK"
     gene            complement(25903..26622)
                     /locus_tag="PK576_00245"
     CDS             complement(25903..26622)
                     /locus_tag="PK576_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE60051.1"
                     /translation="MTSVCAMRDHKSLYRQLIAGMYDALLITDPNGHVIEINPRATEY
                     FQVKPEEVWDKPVAFLIPGVTPQMIGRIRKGIDEARHIMLDAKCQRQDGTVFAAEVTI
                     SVIDLINSGDLVFTVRNTERRRKQWQALRSMANAFENAQSACFVCDRDRVFRCVNPAF
                     LKMFDLREENEVLGRPFEDLMPDEPLPELFNRALGGEPVACRVVADAEEDKRIDLEVQ
                     MGPNRLGRDAVAGVVGSLLQV"
     gene            26802..27059
                     /locus_tag="PK576_00250"
     CDS             26802..27059
                     /locus_tag="PK576_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60052.1"
                     /translation="MEERLDETTFVSFICEACNQEIEAPADLAGRETECPTCGVTLTV
                     PYVSTPGTLWGGPLENTGGPTPAQTEAMKSRTIRIELPDSW"
     gene            27067..28245
                     /locus_tag="PK576_00255"
     CDS             27067..28245
                     /locus_tag="PK576_00255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE60053.1"
                     /translation="MSRILFISPKPYSPWEGACHRTRHILEAQIALGYDVDLLTPTEG
                     QTPTVADSVTTIPLPRLPFCRRTPTLPSLLRLTLDLLALLKILWLAGNTRYALIHGQG
                     NSGVLAWAAARLLRIPCIVELRNDPAPDPAHRRGLIPALYRRLEHKALRQADAVIGHD
                     AATVARLTDLGCHSRACVIADIPAVTEVADAPARNLAKARYRTLTDQDLVTCVASYTR
                     FQGLDLFFNALPRLLATLPHTRCVVVGGGPEEIAQMREALDRAGIHEAVTFPGRLSTS
                     ELAALLAISDVLVSSRRAGATAPIKVLDYLLSGTPIVAVDTPANRAILSPENAVITPA
                     TPEGLADGLIRLLRSPGLRTELRLKGRETLRRENRTPEAFRAALARCYTYALSRSEAE
                     "
     gene            28247..29287
                     /locus_tag="PK576_00260"
     CDS             28247..29287
                     /locus_tag="PK576_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOE60054.1"
                     /translation="MTRRLLRFARSFVILVSLFLLSTLIAANALVHLPRQRQQSLLQN
                     LPDVAAQTLTRVGHAAADITDGLGWTGRDAVAALPQPTDTNRLTRAGVPRRLPSCTAP
                     DDLVVLNKIGFTIGYSPAWRHPVWVAYRADPTCRNPLLPRPSGFKPDPAAPRSPKHRE
                     YSRSGYNRGHMVPNRVIASRHGPAAQAQTFLTSNICPQRASLNQGPWENLEFRIAELW
                     PTRYGSVWVLAGAVPAPEARRLPSGIHVPAAFYQIVVTQPQDGTLRAFAVYMPQHVRR
                     RTPVRSMLVSIDELETLTGFDFLSELPDEVEAELEAATPTRLWPVGLVGTIRLVRERF
                     RRYPVKRVQGAR"
     gene            complement(29272..30108)
                     /locus_tag="PK576_00265"
     CDS             complement(29272..30108)
                     /locus_tag="PK576_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOE60055.1"
                     /translation="MRMGCVLGVIALLVCGAGAAEREGWRLVWADEFDRPGMPDPAKW
                     TYEKGFVRNREAQFYTVARRENARVENGCLVIEGRKETFANPGHKSGSEDWREAETAA
                     YTSASLTTEGLFAVTYGRVEVRAKLPRGRGMWPAIWMLGTSFRSIGWPRCGEIDIMEF
                     VGHAPATVHANVHWFDTTQGKRTSLGGKLHDQKPSDDFHLYVVERFEDRMDFYYDDTL
                     YHRFLLDKAGAGEDNPFRKPHYLLINLAIGGAWGGQKGIDDAMLPQTYEIDYVRVYQR
                     AP"
     gene            complement(30252..32291)
                     /locus_tag="PK576_00270"
     CDS             complement(30252..32291)
                     /locus_tag="PK576_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60056.1"
                     /translation="MRKRGIFVCACLISMVAGAVTNVFEMPLLYPKHLALQQQLMRVV
                     RTGETEEMERICREGVALLPRDPTWQYNLACALAYRADKGEALAALDRAIDLGFRDAA
                     AIEADRDLKSLGPIKAFRELLDKARRLQGKPVEGMPSVRPVSVVMGRPAEVNAENTIW
                     DFEFGCFRTRFNLLVPDLRPVSDYAEAYRGAAAETVRAGFKAGTASGNFGDLYVNRDG
                     NHSLLVVSNYPGLTPVVYGEQAKQAQVHLSLPNGLFDAPVLGNASLSMVSGPYWRSLP
                     RAAVTDPLRAIISLRFYLGNQIWVFPEHRDHDPETGDLYPVNAPFFVISQGSSGSDQP
                     FVNAFAATLAAFTPATKRALTQRNRIGPTLQRILRITQKAVAKDEERYLTGAAHPVVF
                     DAAQLDVEAMVRMAQAMTPEAIPPLVALRTVEDAKVQAGLDFFDLRSEGLFDTPCCIA
                     RVVRGVSRERVMTVEAAVPGQQQEGAFRWTVLQGDTRKIVITPLNANASRVKLTVSYH
                     GAFRPVDDQGMPSALLRHRVDIGCFARHGKGYTAPSIISFYYLPNEVRAYRDDGQVLS
                     VDYTNAEHRYADPMLTFEKGWKDLYAYDDQGRLTGWYRTRPGRTPERFTYLGHKVLQA
                     DSRDRPVQACAVEYLPRQTGGQQTPPTLTCVETRQRFAYSYADDADRIGRATAEP"
     gene            complement(32329..33129)
                     /locus_tag="PK576_00275"
     CDS             complement(32329..33129)
                     /locus_tag="PK576_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOE60057.1"
                     /translation="MKGWLLVLFALTLKACAQFTVEERFHSCVFTNAPTGETFAYRLS
                     APQFPRPGVRYPLILFLHGSGECGTDNRRHLKVGLPALLSTLLRQPEPVMILAPQCQT
                     GNWWVKRLARGEDYAMPPQPAPSMEVVLAMVDHLVDACQADPDRVYITGLSLGGFGTW
                     DAIQRRPERFAAAIPVCGGGDIRRVRELRRMPIWVFHGTADQNVDVACSRRMVQALKQ
                     VGNRAVRYTEFEGAGHAVWDRAYGNDEVIQWLMRQTRAPVPWWRRLFG"
     gene            complement(33254..35377)
                     /gene="hemC"
                     /locus_tag="PK576_00280"
     CDS             complement(33254..35377)
                     /gene="hemC"
                     /locus_tag="PK576_00280"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOE60058.1"
                     /translation="MQTVRAKVGTRGSRLALAQTRDALNRIAALFPFLEFDPVTLETP
                     GDRDLATDLRVSPADFFTRDLDDALRKGCIDLAVHSAKDLPDPVPPDLDWFWLPWGED
                     PRDAWVLPQGKTWADLPEKPVVGVSSERRGAFARARFPHAVLKPIRGAIPSRLEQLDA
                     GRYDALLMAGAALNRLGLEERVSEWIPLSELPVPAGQGRLAVTFRLGDPVWTRVRGYF
                     VKAVRFVGAGVGVAESCTWGGVRDLRQAEVCLYDVLMDDRLLEFLPPSAERVFVGKRC
                     GEHAVRQPDITDLIAKYARQGRRVVRLKGGDPGLFGRLAEETDELERLELPYRVRAGV
                     SALTVATTGTGMLLTKRGVSRGFTAFTPRVEGGAVASVSLEARRRLPLVLFMSVRVAT
                     EMAGQLLAEGWDGKTPVGLVFDAGAEDELIFRTTLAGLREGAAAPVETKAPGLLVIGE
                     AAAGAYRRDTGALRGCRVLLTCSEALLDKTALRVVDFGGVPLLRPLIRLTPCVEAERA
                     LTRIASFDWVVLTSPSAVHLFMGMIRQAGVDLRRLPRVMACGPGSAAAFRHHGIHPDL
                     TPPMEYSAAGLAAEMRTLEIAGCNVLRLRSEKAGPLLADVLREEGAAVEDVLLYVNEP
                     VHYPRLPASDAVFFASASAVEAFMGQAGVEALAGQIVVTIGRPTSEALAGFGRQPDAV
                     AAEATAEGAIEALARVVMSRGLGAS"
     gene            complement(35517..37043)
                     /locus_tag="PK576_00285"
     CDS             complement(35517..37043)
                     /locus_tag="PK576_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE60059.1"
                     /translation="MRDTRPVRKTFPINAMIERRRVLVVGGGRVGQRKVELLLDSGAE
                     VRLVCPDCVAELQTLAETGAIHHTARRFEPADVEGAAMVFACTDDKRVNRTVLESAQA
                     NRILCCCADGNWPDGDFVTPAIIRTGDVLVAVSTSGKSCRQSRLVKENLRKHLDSIEN
                     SDLLVLGTSHEYLSAEERAPFHLLLREREAIGGMIRQIWGVHEFFILNTCNRIEVVAA
                     ISREAGTSGILRRLLRFDQLPPDRFYMKRGFDAFAHLCLVAAGMDSQTPGEFHVVSQV
                     KEAVDEAVTFGWAGSVIRELCDAALHISKDIRHEVEPLLDVAEIEDLALRYLAEQLPA
                     LPDSRVLVIGTGVVGQGLMEDLLRRGCHCVWAYHRNAPASSAQDAHRIEVIQLKDLAR
                     VLPTIDAVVSAVNASEPVLNRLEHGARLNPRGVTLIDLGMPRNIDPALDTFGNGVRVA
                     DLDEIKRWYRSANGTLDKVLKACDAILREHRSLYERMRRSVQGEHETGEERRTLNVER
                     "
     gene            complement(37107..37943)
                     /gene="dapF"
                     /locus_tag="PK576_00290"
     CDS             complement(37107..37943)
                     /gene="dapF"
                     /locus_tag="PK576_00290"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOE60060.1"
                     /translation="MKIRFTKMHGAGNDFVLIDDREGTVPWEDHFLMAALAGRHTGIG
                     CEGVILLQRSDKADFRMRFLNPDGTEVEMCGNGSRCAAAFAHAIGASGRALTMETMCG
                     LVDAQLTKNGVCVWMPEPTRKNYGIEVQIDGETIRGDFLNTGVPHFVVQVPHISAVDV
                     QGLGRALRRHPVFAPDGTNVDFVTLRAPNRMTMRTYERGVEAESGACGTGAVACVVVA
                     VETAGFTLPAKVKTSSGYDLTVDGDWRHRKCTGLTLSGPVKFVFSGEIDLDNMELGDA
                     ID"
     gene            complement(37970..40117)
                     /locus_tag="PK576_00295"
     CDS             complement(37970..40117)
                     /locus_tag="PK576_00295"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOE60061.1"
                     /translation="MMRTCRFFGLALFVLGMMPRIAGAAQTTLVPQPHYGGVAQKVAR
                     VLPAAHLSQKPLNDAISQVAWTNLLNAFDFDHSYFLQSDIDAFIPMRTQIDDALKAGD
                     VSFPFEVYRVFRKRVTERYQAVTNLLEQGFTFDQEESYTWKRREAPWPATLTEQDDLW
                     RKRIKNEVLSQRISRELDRLGVTNAPVQAVTNAMPGAESTVTDTPEQNIAKRYKQLMI
                     VVQDMDEEAVLQRYLSAVAMAYDPHSDYMSPMRKEDFDIDMNLSLCGIGAQLRSEDGA
                     ALVVEIIPGGPADRDKRAQRLTKGDKIIGVGQGNGPIEDIVHLPLNKAVRKIRGEKGT
                     RVVLEVIPASEPSGTITKRIDLIRDEVKLEEQAATGRVARVTSPEGKTVKVGVVRLPT
                     FYGTMDKRPGQAGFRSATLDVARQLAAFNSEQVAGLILDLRNNGGGSLREAISLTGLF
                     VRSGPAVQVREARQLVVLPVPNLDPAMAFRKPVVVLINRASASASEIVAGALQDYGRA
                     VLVGDTQSHGKGTVQTVMPLGSEKMGSMKVTTASFYRINGASTQRRGVAADIVIPSTL
                     DGLDIGEDKMPGALPWSRVESALYIPVSDVAAFVPQLKAFSAQRLADNSRYARYCTLV
                     RHVQELNEKTEVPLEWEARRAMMRAEREMRKIEDEASDASEQEEWEDDVVLDEAMHIL
                     ADLVALSGGGEIPMETEGDLRTRMMRIFGRELP"
     gene            complement(40114..40821)
                     /locus_tag="PK576_00300"
     CDS             complement(40114..40821)
                     /locus_tag="PK576_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOE60062.1"
                     /translation="MQEPESARIYGVPGERARAAGVMRAVGPLLAALFVLGLFSGLLL
                     PRITWEAAGLGLLVTAAALVWAVRDGLAGLDAFFKGARGEERVAWVLSGLPRGFHVFH
                     DLLCGGRSEGIDHVVVGPTGLFAIETKCWSGSVTLEEGVLRVDGGLPSRPPLEQARAS
                     AAAVTSFLAERVGGSPACVPVVCFASNTFRPGVSVCEGCVVCNVSGLAGVMRERGGHL
                     SADEIERAVKVLEQKDV"
     gene            complement(40847..42739)
                     /gene="dxs"
                     /locus_tag="PK576_00305"
     CDS             complement(40847..42739)
                     /gene="dxs"
                     /locus_tag="PK576_00305"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOE60063.1"
                     /translation="MSNLLKTIRTPADVQVLESGQLPVLAEEIREAILETVSRNGGHL
                     ASNLGAVELTIAILRAFSPPQDKVIWDVGHQTYAWKLLTGRRERFHTLRQRDGLSGFT
                     RPEESDCDVCVAGHAGTALSSALGLAAGRERGNGAGHVVAVIGDGAMANGIALEALNS
                     IEEVRSKVIVILNDNEMSISENVGALSRRLGRMLADVRYNRIKAAAEAAGHRLRMTPL
                     RRIYYRLEQAIKSLWLQNAFFEAFGLRYVGPIDGHDFRALENALCSAREYKRSVLIHV
                     ATQKGRGFRPAERRPSAWHGVGAFDLEAGQPPEARPGGYSQAFGETLTALAERDPSVV
                     AITAAMCSGTGLEPFASRHPERFFDVGICEAHAVVFAAGLATAGLRPVFAVYSTFLQR
                     AVDCVMQDVCIPRLPVVFCVDRAGVVGGDGPTHHGVYDIPMLRCLPNLTIMQPKDRVE
                     LEAMLRTALALDGPSVIRYPREPGPEVACDAAVPALSVGQAEVLLHPDAGPEGGEVWI
                     WALGDMLPLARDVATRLRDAGLRSGVVNARFVKPMDTTLLYAQAGNAAQFVTMENGAV
                     SGGFGSALREALAAGGFDCPVSAFGWPDRFVGQGNLAELLEGCGLTAENMARQIAGKK
                     RVATGV"
     gene            complement(42744..44372)
                     /locus_tag="PK576_00310"
     CDS             complement(42744..44372)
                     /locus_tag="PK576_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60064.1"
                     /translation="MLRKGSHRFCLGCAATLLAGATVVLYAFAVWFGNLNQDEGWYLY
                     AAIRVAEGARPYRDFFFTQGPLLPYVYALFSGLWTPFGVLGGRILTALMGLAGCVMAA
                     GLARRAVPAQRAAEAGLIAFALTGCNLYHIYFTTIPKTYALASCLLLGGYGVLSLCVS
                     RRCKTRSALSCMWALPAGLLIAFAAGTRLSLGALLPVTALALLVTCRKTGAAFFWFAL
                     GGGVGLALVYGPCLMEAREAFIFGQTYHVSRGGRDWFYLAGSFSRTVRAYLPMALLTA
                     ALIIFRIFRSGIVGQNAQEEPAGVRYQGGMWPAIWTLGFLAVFLVHLLSPFPYDDYQV
                     PVMGLLAAALAGWVANSTSSGTLRGCLCLFWTVASLLAAFASPLAQEWFVHGQDRFWV
                     VRKPMPDLALLRMTARDVRALAEEDRLLLTQDTYLAVEAGMRVPDGLEMGPFSYFPEL
                     GDEDAQRFHVMNTRRLSELITSASCRVAAFSGYGLAVRAPVMDEVPHAERQMFFSKVG
                     KYYDRVQEVPGFGQNGTTLQIWKRRTSAAEKAAE"
     gene            44626..45543
                     /locus_tag="PK576_00315"
     CDS             44626..45543
                     /locus_tag="PK576_00315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE60065.1"
                     /translation="MNAFPDSVTPPPAALPRDTPGERLQNVLAHRGVASRRHAADIIA
                     NGSVTVNGKVIFAPGFRVDPTRDDIRVNGERLAKTEERSRTILLHKPAGLICSADSSR
                     GQTVCDLMRPHFRERIVPVGRLDKESEGLLLLSNDGELIHRMTHPRYGHSKTYLVRVA
                     GRMAASKIELLRSRLEIDGYTIRPVKVEVLKEGRDNTHLLAFTLSEGRNRQIRKMCSL
                     AHFVVLNLTRVRIGPLKIGTMRPGEWRELSEAEVRALKRTPSASVIPPPDRGGPTRGG
                     TPPHARPKPDLRPPGPRKSGPSARCARHP"
     gene            complement(45485..46474)
                     /locus_tag="PK576_00320"
     CDS             complement(45485..46474)
                     /locus_tag="PK576_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE60066.1"
                     /translation="MDMQMNRRAFMMGVAAAGAVTGMPRFGVAQEGGGACAFRTTLYK
                     ALIAGVADDKTCERIAKAGFPGVELSNKNVTEEQAIQGRLTAAKHGLAIHSFMGGWTN
                     FNSSDEAQRRKAIEDVKRMIRLTATYGSSVILLVPCRVGGTMPKPSQFRIAFDPRTLR
                     VTSVVEGDNAPFAEYIAAQNQATEMSRRAIEELIPVAAREGVIIALENVWNNLWVLPD
                     FAAAFVRSFDNPWVKAYFDLGNNVRYAPTEEWLRALGSQIVKLHIKDFKIDREKKNEG
                     DFVPIGKGSVDWRSVRKVIDEIGYNGWVTIESRGFTDAEHSALMDRFFAGQGA"
     assembly_gap    46637..46736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46906..49452
                     /locus_tag="PK576_00325"
     CDS             46906..49452
                     /locus_tag="PK576_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOE60067.1"
                     /translation="MNRNAGMLLAIVLSLQVRAAGDPAAVAAWEKSGRAETLEWFKRH
                     WFGVRPAERLADQEIGEDYVACHGGQIRINIYVKLPEGASAENPVPAFIFGDHYNPKT
                     SRTKTDTMPGIPTNAIVSRGYAYIRFNFNDVTPNTNQGCHDPSSANMKLGGMGNPDSW
                     GTISAWAWGFSRVMDWVETRPELDAKRIAVIGHSRGGKTALWAGCTDQRIALTISNCS
                     GTGGARYLHMPLKGMETLDWMKDHATRHWFCPSWIPTYRNRETEIEHDADEMIMLIAP
                     RRVYVASGSEDSWAGPAGEFESAKRASALWEAYGHKGLSLDTFPVPGTVDHSGRVGYH
                     LRPGAHAIRPWDWDRYMDFMDKHLPGPKKAPPKAMEPAAPESQRVQSEAILAWINALE
                     AFFDGGEQGSVHGFSILRHGKVIAEGSWKPFDTLHETHMLYSLSKSFASTAIGLLVDD
                     GKLDLDERITDLFPEYVPAEPSENLKAMRVRDLLSMNVGTSHADPHKKDPGGDWVKMF
                     VTNVIDGKPGQAFRYDSSATHVLVAIAEKRSGRTFLDFIGERLFDRIGIGKNAWTTYS
                     PTGIACGGWGLNLTTRDLARFGQLYLQRGVWNGERVLSREWVELATARHTWNGGIVGT
                     RPDDDWAQGYGFQFWRCVPEGVYRADGAYGQYTIVLPDQDMVVSMHAGVKSMGKILKL
                     TWEHLLPGVRNVPLPVNTVGEEALQKRLGGLAIAPVKGSVAEGAYFGRELALKENNRG
                     IRSIRLDREGEGWKCTLVTRAGENAFPVGCGVWAKGTIRIDTEKYEKPGGLIGVQPTV
                     ASGGVDADGVFRMKVFLTGTTAYLSFEISEKQILGRLFAIDGCRFEGKTE"
BASE COUNT         9814 a        17254 c        13716 g         8580 t
ORIGIN      
        1 gcgcctcatc tacttccggc acgagaatcg agacctcttg cggttcgcca tccggcgtcc
       61 ctttgagaaa aaggtggacg ttcggctcct cgacatcgca gtccagcaac gtcaccgcac
      121 cgtgtacacg tgccagattg gcagcgaccg tagttttgcc tgtaccgcct ttgccggatg
      181 ctatcgctat aatcattgtt tcttctttgc ctttgtcgcg ccctgcagcc ataccagcgc
      241 ctgggtgccg agcgccagtc ccacgtggtt tgctatgaca gggcacttga ccttaaacag
      301 aaagcagatg tcacgcggcg cattgctcag tgtctcgaaa tttccttggc ccttggaaat
      361 gaccaagtcg gcggcgtcaa agtgcttacg gaaatctggc gcgcactctt ccagcagcgt
      421 gcccggcaca tcagagccat tggatatgac ctcgaccaat tcatgcagtc cggcggcgcg
      481 cgcgtcttcc atcgtagcat cgttgatgat cggcgcgccg cgcacggcca cggttacgcg
      541 ctcggggccc agcgcctcga tcagcaggcg atccagcacg atttcacccg cgttatcggc
      601 cagatatagg attgttcgcg ctttgccggc acgctgccgt aaagcagcaa catctcctga
      661 aaacggttct ttctcgacat ccctgatggc cgactcgacc tcggcagtcg tcaggtcgct
      721 cttggcgccg aggtcgatca ggttgccgac aatggcgagg cgcacggcca gcgtgaacgg
      781 gtcgtccgag gcggccagcc gctcgcgcag tcccggcaac agtcgcaacg ccatttcatt
      841 gtcacgcgct ttggcttcag catacggatc agcaacccca gtcagttcgc gcaaataacg
      901 gtacaactgc cgggccatga ccggcggcga ctcgctgagg tcaacgttgc ggacccattc
      961 agccgcctgc cgcacaacct tctcatggaa agccgtatca tccgacacgc ggcgcaccgc
     1021 atccagcgtc tgccggaaga aacaaggcaa gcaatccagc gaagtgttca taaggattac
     1081 gcccagtgcc ctcccacgtc agccgactta agttctgtca gtgaaccgtc tcgatacagc
     1141 ttcagcgcct ctgccacggt cgccgcgttt gatgtgtaga ccttgatacc cgcggtattc
     1201 agcacctgga aagccttagg gccgcagtgt ccggagacca ctgcttgcgc acctgcggcc
     1261 gcaacattct gcgccgcctg gatgcctgca ccttgcgcgg cattcaggtt ttgctcgttg
     1321 tcgatgatga tgaaagcctc agttgcggtg tcgtacacca gaaacttcgc ggcccgtccg
     1381 aaacgcatgt ccaacggcgc gttcaggtca ctgccggatg ttgtaaaagc aattttcatg
     1441 attcctcctt tcccgcaggc gcgccttcaa agcgcgccca ccccggccgg cccgtggcga
     1501 aaaacatgtt cctgcatctg cggcctccgc caccccagtt accgcgtccg cggccaaagc
     1561 cgcagaagcg tccgcccgcc ataatgtttc ccgccgcgcc gcctcctgcg caatatccgg
     1621 ctccgcgtcc ggtcatcggt cccattccca agggtcccat tctgtttcca cccggcatag
     1681 tgtacctcct tttttgtctt ttactaattc cgccacggcg tgccgtgggg ctataaaaaa
     1741 acctccgtgc cttctgtgag agacctccac ggcccccgcc aaacaaacta acgcactaac
     1801 gcactaatgc actaattaac taatgcactg ctccctgccc ctgcctctgc ctaagcctct
     1861 gcccgcgcct ctgccgccgc ctgcgcaggc gcgccgcatc cgccgccgcc cgcaacagcc
     1921 cggcatcgcg aagaccgcac tgttcaacaa accgctaagc gcggctgata tgacctgcct
     1981 gacctctcca gtcacgaagg cctcggtctg tatgcccgcc gcaatgatca tctcatgcag
     2041 cggacgcgag accgcgccgc ataccagacg ctttacgcct tgcgactgca gccatgcgat
     2101 atcctcggcc ggggtgcgcc cttccagttt gtgcaggctt tcgccgcaga tctcctgccc
     2161 ccgcgactcg acgatcagga tgtccctggc cgtgtcaaaa accggcgcga tacgggttcc
     2221 gtaaatggag tatgctgttt tcacgcctta aatatatgca atccgcgtgc caagtggcat
     2281 cggctaggtt atatcgatgt aaccatctgt ccagcaatca gttatgacgt catcggaggc
     2341 gaggcgtgaa ttttcactag ttgcatttat gctactttac cacaaattgg gttgcattta
     2401 tgctaccatt ctgaagcgct tgatccataa gggtaaaacc gggagaagca agcgtctgct
     2461 tgcccggacg aacagacgct cgtcccagaa aaattccgaa ctccgcgcct agcgcggtga
     2521 agcacgctct attctctccc ctattttctc tctcaatgtt caaatacgat cccgatcccg
     2581 atagcgataa aacccgaaac caaaacaaag cgggtaacgg atcagatgcc ttcaagtccc
     2641 aacgccttga tcttgcggaa gagcgtggtc ttgtgtatgc ccagcgcgcg ggcggcggcc
     2701 tggcggttcc cgttgctgcg ctctaaagcg agacgtatgg cctgcgcctc ggcgatggcg
     2761 cgggtgtcct tgatatgacc gctggctgcg gtctgtgtcc gtgtcatctt tgcgcgcgtc
     2821 aggttctcgg gcaggtgttg caaaccgatg ttttccgcgc cgcagaggat gaaggcgcgt
     2881 tcgatcacgt tttccaactc gcgcacgtta cccggccagt cgtgcgccat cagcaacgac
     2941 agcgccttgg ctgagatacc tgtcacacgc ttgctctgca ggcggttgaa acggcggatg
     3001 aattcgtcca ccagcatcgg aatgtcttct ttgcgtttgc gcagcggcgg cagttccaca
     3061 cgcaccacgt tgatgcggta ataaagatcc tcgcggaacg cgccgtcgcg gcagagctgc
     3121 tgcaagtcgc ggttggtcgc cacgaccacg cgcacatcgg ctttctcgga acgggtggcc
     3181 cccagcggct cgtagacccg ctcctgcaaa acccgcagca ggcgcacctg caacgcagga
     3241 ctgatctcgc cgatctcgtc gagaaagagc gtgccgccct tggccagcgc gaagcgtccg
     3301 ggcttgttcc gtgtcgcgcc ggtgaaagcc ccggccttgt atccgaacaa ctccgactcc
     3361 aacaaggtgt cgggcagcgc gccgcagttg accgccacga acggcgcctg ggcgcgcgga
     3421 ctcagcgtgt gcagcgtccg ggcgaccagc tctttgccgg taccggtctc gccctgcacc
     3481 agcaccgtgc tcggactggc ggcgatcgcg ggcaacacct caaacaggcg ctgcatcacc
     3541 ggactgcggc tgaccagatc gccgacctga aaccggccct ccagctcgcg ccgcagggca
     3601 tccacctcgg aaagatcgcg gaacacctcc gcgccgccaa tgacccgacc cgcatgatcc
     3661 ttcagcaccg cggtggagat gctgatcgga atgcgatccc cttcggcgtt gatgatgtat
     3721 cccgagcggc cgatcaccgg ccgaccggtc ttcaaggtcg tttgtaacgc gcattcgggc
     3781 ccgcacatgt tggaacggaa gacgtccgcg catcgacggc cgatcgcctc tttccgcggc
     3841 acccctgtaa tggtctcggc cgcccggtta aacgaggtga cgcgccacgc tttttcgaag
     3901 cagaagacgg catacgagat gctttccaga atggcgcgtg tgcccatgtc gccgtctgtc
     3961 tgccgtatct gggaccgacc catgcccaca cccttcccgt ctgattccgg cctatttttc
     4021 cggttcgatt gcgtatccga gcgtcacgac cgctttggcc gttttctcga tcgtcgacgt
     4081 gtcgtacgcg ccgtatccgg acgtgtccgt ggagtggcgt tcggttatct gaaaaacccc
     4141 ctgttgcgcg gagatcaagc tcccgaccct gcccttgctg ttggcggcaa gggccaccgc
     4201 acgccgatac ccgtctgatg tcgcttcggc gagaagcgcc agtttcgtat ccttcaggtc
     4261 ggagatgtag aactcggggc tcccggcccg gatttcgatc ccgtccttga tcaattcggt
     4321 gattccgttc gaaacgtcgc ggatcagccg gacattgccg gacgtgacgg tgaaggtctg
     4381 cgtggcgtcg aaatactcgg tttcattggt ttccttcccc tgcgcatccc gtttgggaat
     4441 cttctcggtc tgaatggttc ccgcaaccag ctccgcctcg ccaaagcctg accgcttgag
     4501 gtacgccatg accgcattcc tgctttcccg cagtttgtcg taggactcct tcagactgga
     4561 acccctgacc gtgcatgtgg cggtgaactt cccgacgtcg gacgtaatat ccttttccgc
     4621 gtacccctta accgtaatgg tctgttcgtg ccgaatccgg acgaacagtt tggacaacag
     4681 ggaagaggaa agaatcatcc ccaaagccag gatcaccgca gccgcagata ttttgacaac
     4741 gctattcatt cgtctctccg tcaaccctgt tttttcatct gggggaagag gatcacatcc
     4801 cggatcactt cggttccggt gagcatcata accagccggt cgacccccat gcccaaccct
     4861 ccggtcggag gcatgccgtg ctccaacgcc gtgatgaaat cctcatcgat tttttcgggg
     4921 ttatcccccg cctgatcagc cagcgccgcg cgctgcgcca tgggatcgtt ctgttcggta
     4981 taacccggac aaagctcttt gccagccacc acgaactcga agacgtccgc caactcggga
     5041 tcgtccgcgc aggttttggc cagaggcacg aactggcgcg ggatgcgcgt cacgaacgtg
     5101 ggctgacgca gcattttctc gaccactttg ccgtagacct catgcgtgac catcagatgg
     5161 ttccacgacg ggtcgatgtc cacccccttc gcctcagcac gcgcccgcgc ctcgtccacc
     5221 ggcagatcaa accagtccgg ccccatcacc tccctcaccg cgtcccgata cgcgatctcg
     5281 cggtagggcg gggtgaagtc cagcatctcc ttggcttcac cgtacgccac ctgcagcgtg
     5341 ccgagcacct tttcgcacag gtgcggcatg agtccttcaa tgaggctctt catggaggaa
     5401 cggtcgccgt aggcctcgta gacctccagc acggtgaact ccggattgtg tgtgcggtcg
     5461 atcccctcgt tccggaaatc tttgcccaac tcgaagatgc ggtcgtatcc gccgacaatc
     5521 aaccgcttca aatacaactc aggcgcgata cgcatcacca tctcggtgtc cagcgcgttg
     5581 tgatgcgtga cgaacggttt ggcagccgca ccccccgcct gcggctgcat gatcggcgtc
     5641 tcgacctcga tgaacccgcg cgcgtgcagg aaagcgcgga tctccgccag aatcgcgctc
     5701 cgcttatcga accgggcacg cgactcggga ttcatcatca gatccagata acgtttgcgg
     5761 taacgctctt cgacatcctg caacccgtgg aacttctcgg gcggttgcag gatcgccttg
     5821 cccagcagaa cccacgtgtg gacctcaagg gtcggctcgc cggtacgcgt cgtgaagagg
     5881 cgaccctcga caccaatgaa atcccccaaa tcgagatagg cgaacgcatc gaactgttcc
     5941 ggggtcaacc gatccttctt gacgaaaatc tgaaaattgc cggtgccgtc gctgaacgtt
     6001 gcgaacgagg ccttgcccat gcgccggatg gtcatcaggc gaccggccgc ccgcaccacg
     6061 cgatcctccg taaacgtgga gcggatttcc gaaaggcgcg tgcgctcaaa cgcctgcccg
     6121 tagggcgcaa acccccgctc ctccagactg cgcagattag cgatgcgctg tgcgcgatac
     6181 tcgtttcccg actgcggctg ctcctctgac atctcgaccg tccctttcct ttcaaccatg
     6241 cgcggcagtg tatcacacct tcgggagccc tgccactact cgcgttccca atacgccgcc
     6301 tcgttggtgg ccaaccgccc cgcctccatc tggcgcaaca cgcgctcgga atcgatccgg
     6361 cgcggaatgc cgggcaggca cgcgtccgcg gcaggcttgg aggagcgcgg cccctcccct
     6421 tccttcatgc gaacgccgcg tgccgcggga accgcaacgg atgccgcgcc cccgacaccg
     6481 ccgggcacct cttccggccg cgcttgcgca agttcgcgcc attgagccac gcaaaccgcg
     6541 caagccagca tcagcgccgt gccggcactc agccgggccg cgcgactgcc gccgaacgcc
     6601 agacagaccg cgaagccgag cgccaaagcc gcaaaagtgc caatcgtccg ctccaacccg
     6661 gcgagcggca ccaataccag aaagaacatc cccgccgccg ccgcacccaa cgcatcggcc
     6721 agaacaaaga ccgcggcgcg attctccggt ccttccccgc agcacgccca cgccacgggt
     6781 agcgcgcctc cggccgcgac cccgatcgga aaacagagca gcagggttac ataagcggtg
     6841 gcggcgcgtt ccgcgccagc cgacatcgcg aaggcacatc ccgccagcag cacggtcaag
     6901 gccgacggta acgcagccgt gacaacggga cgctccggca tggcgcgcat ccccccttcg
     6961 gccaaacggt tgccgcaaaa aagccctgcc agatacaggc agctcccggc acccgctaac
     7021 aggtagagcg aaccgaaacg catctcgagc cggctcagca ccgtgagcga cgcggtcagc
     7081 cccaaagcgc cgcacgccgc gagccatgcg gccaccatcc ggcgcggcgc ggtctgggcg
     7141 cgccccacga ccaccggcac ggtccagagg atcagcagca gataggctgt cagacggaca
     7201 ccctcgggcc gcagcaaccg caggaaaacg gatcccgccg cagtttcggg atacgtgcgg
     7261 ctcaaggcgt cagccaagcc gtaggccaac gtcgtttccg gcgcggacat ctccggcggg
     7321 gccagggggg ggtccgtggc aagccacgca ggccatcgcg ttcgccatgc ttccgcgcgc
     7381 gcgggatcat agagcaggcg cactgcctcg gccggatagt gcgcactctt gagcagggcg
     7441 aaacgacgta ccgcgtcttc cgctccatac gccaatcccg gcacctgcgc agccacccac
     7501 caccccccgg caccggccgc aagcgcgccg ctttccggcc agacctgccg gatccgccgg
     7561 acaaagcgct ccagcaacgc gccctgttca tgcgtcacga cggcgcttgt gcaatcgagc
     7621 ccgaacaacg caacgccggt acgccgcgtc acctgccgca ccgcacacag aaaggccgtc
     7681 tcgcgccata ccgcgccggc cagagacgtg gcggcgggcg gccacaccaa caccgcatca
     7741 aaaagaggcg atgcgggatc agccagataa cgccgcggcg tgacaccggc cgcgcggata
     7801 cgggttgcca tacccgccgc cttcacgctt ttcagcaacc cgacaccata gccagggtcc
     7861 gccgggcacc acacgatttc cagatccgga cgcagcgact ccagcgcgag tcccgttgcc
     7921 agcgacacct cgcccagcaa cagcaccgtg cgggcgtaag ggcgctgcga caacagcagg
     7981 gcggcccatc ccattgcgta gtcggtttcc ggaatcacct cccggacccc gccagaggag
     8041 agggcataga aggtgccgcg gtgtttcccg tagaacgtcg taccgccgcc tgtctcaaac
     8101 cgtccggacg gcgcggtgcc gtcggggaag gcgcgccgcc actccgcaag gtcgcgcggg
     8161 tccggcgtga tccccaaggc cagcaagcca gtcacggcaa gacctcccag cgcagcgccg
     8221 agcgcctccc aggcaaaggc ccggctcacc ggggatccgt cgcgcgccaa ccggaccgcc
     8281 accgcaggca acacaaaccc ggagacgaag caaaaaggga agttcgccac aaagcaaccc
     8341 gccgcaagcc tcgccaaggg gaaggcctga taatcgggaa cccccaacca cgcgcgcaaa
     8401 tttccgatca gcgcgtattg gacaaaatag agcgcgatgc ccgcagacaa cggcagccag
     8461 aggcggtcgg ccaatatccc cgtaatccgg cggcccatac gcgtcgcagc gactgcggca
     8521 cccagcccgg tccaaccgag ccagcaggcg agaaacaagg ccaccccgct ttcggtcgcc
     8581 gcaaaccgcc agaggaaacg ccgcaacagc aacgtctgcg ccaccatggc gacagcgcca
     8641 aaaaccgctg ccgtagccag tatcgaaaat gaacgaagct tcctcaccct tcataccctc
     8701 tatgccgtga acattgagcg ttcgttccgc ctttgggagg gacgcccgcg ctccagactc
     8761 cgcaccgcat attccccgcc ctcttcacga ttcacgactc aaacatcctc aaacttttgt
     8821 cccggctgcg gcgccaccac gttctctacc ccgcgcgccc ggaccaactc gatcatggcc
     8881 tggacttttt ccggttcgcc gtggaccgcg aacacccgct tcagacgctt gccgcggacc
     8941 gcgtcgaacc accgcatcag agccgtacgg tccgcatgcg cagacagtgc gttgatcgtg
     9001 tggacgcgcg cgttcagttc aaactcatcc cccagaatct ttaacggcga acggcgttcg
     9061 acgatccggc ggcccagagt gttttccgcc tgaaacccga cgatcagaat gatgttgcgc
     9121 ggatcgccca cgccctgtct gagatggtgg agaatacgtc cgccctcgca catgccggac
     9181 gcggcgatga tgatcatcgg ccccggccgg tcgttcaacg ctttcgactg atcgggcgtc
     9241 tccacaaact gcaagcccgg aaagcgcagc gggtcgatcc cctgccgcaa cagatcagag
     9301 gcctcctcgt cgtagcactc acggtgccgg gcatagacct gggtggcctt cagcgagagc
     9361 ggactgtcca catacaccgg cgtgagcggc atgcgccgct gttcgatcag ccggttcaac
     9421 tcgtagagca gatgctgggt acgtcccaca ctgaacgccg gaatgatgag tctggctttc
     9481 tgctggtgga tatcctcaac gaatcctttg agccgacccg aaacgtcctc tcgcgcaggg
     9541 tggtcccggt ccgcatatgt gctttcaatc agaatcgtgt ccgcgccctg cggaaaatcg
     9601 taatcgcgca gaatcggtcg gtcgggctgg cccagatcgc cggtgaacaa cagccgccgc
     9661 gacgtcccgc cgcgcgtctt cacatccaac tgcacgagcg cagagccgag aatgtgtccg
     9721 gcgttgtgca acaacaagga gattccgtcc cccagatcga ccagccgttt gagcggttcc
     9781 ggttcgaagc gggagaggat gtggtccaca tcctcctgct catacagcgg ctcgaacaga
     9841 accttgcctt gctgcgcacg ttttttgttc acgtattcca gatcgcgctg ttggaggtac
     9901 gcggagtcgc gcagcatgat gtcgcacagg tcgcgcgtgg ctggcgtgca gtacacctta
     9961 ccccggaacc cgtgccgcgc cagagtcggc aggttaccgc tatgatcgat atgggcgtgc
    10021 gacaggacca cggcgtccag cgtccgggga tcgatcggca tgttgcggtt cttttcaaac
    10081 gcctctttcc ggtgtccttg gaagagaccg caatccagca gaatccgctt cccgttcgcc
    10141 tccaccagat gcatcgaacc cgtggtcgtt tgcgccgcgc cgaagaatgt gagtttcatc
    10201 atcccgcttc ctttaactga tggttctgat ccgggaccag tatataccca tcccgtcatt
    10261 caatccagcg caacgcgaga ataacaggaa cgccgaacgc cggtatggca cgggcatctt
    10321 gcccgtgttt catgggcggg acacccatgc cgcaaaattc ttcgatccat gcccccaaaa
    10381 aatcttcgtg ttcttcgcgt cttaccgccg tcatcccgcg gccccagcgc accaaacgtc
    10441 ccgcatctct catcacgcac cctttcaacg cgggcttctc ccgcaacctg atcggcacaa
    10501 cctgtttgga atcggcaggg cgtacggtct caggcgcaga gttcagaaca ggtcatcgtc
    10561 ctcctcatcc gcaccgccga aaccgccatc cccctcttcg tcgaaatccg cgccgtacag
    10621 cacatcctcc cccccgtcct cttcctcctc ttcgacagcg aagagccgac tgcgaagcgc
    10681 accggaaccc tcttccgtga cgttgaaccg gcagtcgaac tcatcgcagg cggaacaggc
    10741 cctgggcgtg aacgccgcag ccgaggtttc gccgtccaac accagcggac aggttcgcgc
    10801 ggtcggcccc gcgaccgcgc cggtcgatgg tgccgcaggc ctctcgacgg caacgggcgg
    10861 acgcacaccc gcagcggaaa ccggttgcgg cgcggcctta cggggttccg gtttcgcggc
    10921 ggcccgcgcc gcagttgcag ccgttttttt cccggccggt ttcacagatg tccgtttgac
    10981 ggatcgtttg ctcgtcgcca tatcaggaat cctgcctttc cgcgggttca gccccgcaac
    11041 agcgtcaggc cttctccaca tgcaggccga ccgcgtgccc gttcaactcg atcaccggcc
    11101 catgcacttc atcgaaccgg atgccctcac acagcgtctc ggctttcaca taatccgcgt
    11161 actgttcgat cgcctcgcgc acctcggccc cgcacgccac cgtaacccgg atacgctgcg
    11221 tcacctcgta ccccgcctct ttgcgcaggt tctgcacacg gctgacccac tcacgcgcca
    11281 acccctcgcg gatcaactca ggcgtcagcg cggtctcgat cccaacgatc acatcgtcct
    11341 cagaggccac gaccatcccc actttgggag tccgccgcac caccacgtcc ccggcacaaa
    11401 gggtcatcga ttcgcccccg accgcaatcg aaacctcgcg ccccgcggca agcgcggcag
    11461 cctcggcaga ggaaagagcg gcgatggccg ccgcgaccgc cttcatgcga gggccgaccc
    11521 ggggacccag ctgacgaaaa tcggctttca ccgagacatc agccataacg gtctcgtcct
    11581 ccccatatgc cacggttttg atgttcagct catcggtgat cagatcctca tacccctgca
    11641 ggatcgcgcg aagaccggca ttcccggaga ccacatgcag caccgcgagg ggctgccgaa
    11701 tcttcagatc gttctcggta cgcaactggc ggccaagccg caccacctgc tgcacccggg
    11761 ccatcgcgct ttccaacgcc tcatcacggc ccgccgcata cgcctgcgga aaatcgcaca
    11821 gatgcaccga ctcgggcatt gaggcgccgc gcagattacg gtatatcgcc tcgctgatga
    11881 acggcgtgaa cggtgcggcc acctgacaga gctgcaccag cacataccgc aaggtccggt
    11941 aggcgtgcgt cttgtcttcg tcgttctgag atttccagaa gcggcggcgg ctccgccgga
    12001 tataccagtt ggtcagatcc tcaataaacg tgacgaacgg gcgcacggaa cgctgcaagt
    12061 cgtatccgtc catcgccacc gtcacatccc ggatcagcgt ctcgagcgaa ctgacgatcc
    12121 agcggtccag cacattcgga ctctcgggac ggaccacctc ggcatcgtca aaccggtcga
    12181 cgttcgcgta cgtgacgaaa aagctgtagg cgttccacca tgggatcagc aattcgcgca
    12241 gaatctgttt tacgccggtt tcggagaaac gcaagttctc ggcgcgcacc accggcgaat
    12301 aaatcatgta gagccgcagc gcgtcggccc cgtacgcatc gagaatgtgc gacgggtccg
    12361 ggtagttctt gaggcgcttg gacatcttct tgccgtcctc agccagaacc agaccgttga
    12421 cgatcacgtt cttgaagggc gactggtcga agagacaggt ccccagcacc atcagggtat
    12481 agaaccagcc gcgggtctga tccaaacctt ccgcgatgaa gtccgccggg aagaatgtgg
    12541 agatgtcctg cccgcccttc cctctcttct cctcaactcc taaatcctca actcctaaat
    12601 cctcactcct cactcctaaa tccccgaagg ggtaatgctg ctgcgcgtat ggcatggctc
    12661 ccgactcgaa ccagcagtca agcacctcgg gcgtgcgccg gtagaccttg ccgtcgcgcc
    12721 ggatcacaac gcggtccacg aaatgcttgt gcaaatccgt gacgcgttct ccggaaagct
    12781 gctccaattc cgccaccgaa cccacgcaga tctgatccgc gggatcattc tcattgatcc
    12841 aaaccggaat gcaggatccc cagaaacggt tacggctgat gttccagtcg cgcgcctcgt
    12901 tcagccagtt gccgaaccgc ttttcgccga catactccgg catccaacgg accgtagcgt
    12961 tgttgcgcgt cagccgctca tgcagatcct cgacgcgcac gtaccaggcc tcgatagcgc
    13021 gatagatgag cggcgtgtcc gtacggtcgc agaagggata actgtgcgtc acagtagtct
    13081 gatgcaccaa cttgccttca cgcttgagcc actggatcag atccttgtcg caatccttgc
    13141 agaaccgccc ctgacactca ggcaccgcct cggtgaaaca acaagcggca tccaatggat
    13201 ccgcgatgac gctcaaaccg gcctgctggc agacccggaa atcgtcttcg ccataagccg
    13261 gcgccatgtg caccagtccg gttccgtcct ccgtgctcac gaagctgtca gtcagcacac
    13321 ggaacgcgcc tgcgtcccga tactccgcaa aatgagggaa gatcggctcg taggtccaat
    13381 cccgcaacgc ctcgcccttg agacgcgcca gcacctcgta cgcgcccgca tccttgtaat
    13441 acaccggaag ccgggcctcc gccagcacaa agacggtctc gtcctcacag tcgcgcacca
    13501 ccacgtagtc gatctccgga ccgacgcaga tcgccagatt ctcgggcagg gtccacggtg
    13561 tggtcgtcca aatcagcaga taggtcgtac cccatgcggg ctcggccccc gaacgcacgc
    13621 gcacacgaac ggtcacagcg ggatcctgca cgtccttgta gttgttgccc gcctcgaaat
    13681 tgctgagcgg cgtgctgagt ttccaactgt acggcatgat ccggtaggat ttatagatac
    13741 gcccctgatc ccagagctgc ttgaacaccc accagatgct ctccatgaaa ccggggtcca
    13801 tggttttgta atcgttgtcg aaatcgaccc agcgccccat gcgcgtaacg gtcttgcgcc
    13861 actcttcgac atacgtgagc accatggagc ggcactgctc gttaaaggcg gcgacgccga
    13921 actcccgaat cgcctgcgcg ccgctgaccc ccagagcctc ctgcgccaag gcctcgatcg
    13981 gcagtccgtg gcaatcccaa ccgaaccggc gttccacatg gtggccgcgc atcgtctgat
    14041 accgcggcac gatgtccttg atcgtacccg ccagcaaatg cccgtagtgc ggcaaaccgg
    14101 tcgcaaaagg cggcccatca taaaaaacgt actcgcgttg tccccggcgc tgctccacgc
    14161 ttttccggaa ggtgtcgtgc gactcccaaa acgccagcat gtcacgttcc atgccgggaa
    14221 aatccgtttg cttcgataca ggttcaaaca taagattcac tcatttcccc cttccgctca
    14281 ccccatggcg cacgaaaggt caagatgcgt aaaagcaaga taggaaaact ataaaggaac
    14341 tgccttgaca cggcaagacc gtataagaac ttaacaacct ataccgtgta acctgtaacc
    14401 tgaaaaccgt ccccgtcagc cattagctat tgcaacacct ttaaccctct gaacgtcaac
    14461 aagtaagctt ttccgattca acggaaaccc ccacagacac acggttttcg gcggatcgag
    14521 aaagcgtaac atcctctgaa ctaaaacgtt gaaggcgtta cacccccgtc gcctgtgcgg
    14581 tcccgcttcc gtccacatat catgagaagt ggacagccct gcacagaacc agcgggaacc
    14641 attcgaggtt tgcgttacgg ttttggtttc tctgctcgtc gggtcatgga gttcacctca
    14701 ttccacgcac ccggcgtctt gtcatgcttg atgaggaatg cctgcaaatc cttcggctga
    14761 gctgttaaga ggcgccttct tcccttcgtg gtcaacaatc cgcaacatgt aattcttccc
    14821 gtcaccctct gtgaacgtcc acttactctt atccgtatta tccttgcctc tccactcgcc
    14881 aatcagcaaa ggatcaaaga tcagatcctg ctccgtgtat aacgggtgca gggatggagc
    14941 acaccccatc aaaaaaccaa gatcccgagg gtcatgatcg cagtcgtctt tttcatgttt
    15001 tgccctttca ttcagacgcg ccgcgccgga cccgcgaccg cgcgccgtaa agcgagccac
    15061 agcacatcca gcagcatccg ccccccgctg gagaagaccc gaaccgtcga atgcccgccg
    15121 taattccaaa tcaccggaac ctccacaacc cggtacccca aatcccgcgc catcgcgatg
    15181 atctcgacat caaaggcgaa gcggttgatg cgcgaacggc tgaacacctc gcgggcgaca
    15241 tcccgccgga acagtttgaa cccgcattgc gtgtcgcgca tgtcgtgaac cccggcaaga
    15301 cgcacaagaa ggttgaaaac ccggctcaac accctgcggt gcaacggcgg aggcgtaaga
    15361 atatgagcgc cgcggaccgc ccgcgaccca caggcaatcg ccgcgcccgc atccaccgcc
    15421 tctttcaacc ggacccactc ttgcaacggt gtggagaggt cggcgtccga catcaggatc
    15481 agatcgccct cggcagcgag cacaccggtc cgaaccgcgt agcccttgcc ccggttgacc
    15541 ttgtagccca gcagccggat ccggtcactg cccgaagcgg cgatgaactc gcgcaccaca
    15601 tccgaacacc ggtcgggtga accgtcatcc accaatatcg cgtcaacctt cacctcggtc
    15661 gtgtcgcgga taaacgccgc gacttgttcc agcgtcccct ttaaccgacg gtactcccga
    15721 taaaccggta tgacgactgt taaccgcatg cctctctttc ccctttgagt gttatccctc
    15781 gtttgttcgc aacgcattcc gcacagcgga cggatcccgc ctttccctca cccgcctgta
    15841 agtagcgcga acgcctcgtg agcggttttc gcggccaaca acccgtccaa cgtctgaccg
    15901 gtcaagcgtt gccgcaactc ggcttccacc tcagcgtaga cagtggcctc ggattccgga
    15961 tacaacacca tcacccacac gcgcggagat gttccggagc cagatgtcgc ggaagcgatt
    16021 cccaacgccg cgaccatgcg gtcgacatgc gccgtccgta ccggaaatac cgcgacctcc
    16081 tcaccgatcc ggaccggcgg cgtctccacg ccccggcgga tatccgccat cgcctccggc
    16141 aacgcattga ccgccccgct gcgcgcgcag agcgccaaca gcagggcaag accctgttga
    16201 cgatcctcca catcgaaatc gagggcgatg ctctgccaac ggaggcaacg cgccagaatc
    16261 gtctgccgtg cgcaaaacgc cgcgccggcg cggccgccgg tgagcaccgc atacatgtcg
    16321 tccggcgtgt cgcacgccag cagggcggcg cggccgtggc tgtccagcag ccgacacagg
    16381 tgcgcgatca cctgcaattg cggcgacgcc gcctgttcgg gagccagcac cagcgccacg
    16441 atacgggctg ggcggccgtc cacggtgtca tatgccaaac cttctttttt caggccgacc
    16501 gcacacacca gacgcgtcac ggcatcggtg cgggcgtgcg gcagggccag cccgtgctcc
    16561 aaccccgtgg agagattctg ctcccgatcc aaaaccgcct gcaacgcgcg atcccggtct
    16621 gtgaccagtc cgctctcgca taacaggttg acgagttcgg cgaccgcccc ggccggagtg
    16681 gaggccagca accgcggacg gcaggtctcg gggaacaggt ggttccgcaa cacacccgcg
    16741 ctcccggccg cagacgcttc cggcacctgt atgttttttc tgagcaacac gtcatccagc
    16801 ggtctccgca acgcacaaag gttcccctcg atatcggaaa gcgcttccag catcactaca
    16861 ttcaccagcg cgcgctcgcg ttcgccgcat tcgaacgtga tctccccgtc atggctctgg
    16921 agatggatca cctgagtctc gcggctgatg cggtagagca tgtgatgccg gtttaacacc
    16981 tgcgcgtaaa agccctcatc ctcaaacagc tcaacaatcc ggttcgccat aagctgcgcc
    17041 gcctgccgcg aagggaaccg gaacaccagc cgcgccggct ccggcccgct tccaccgcaa
    17101 ccatccccct gatccaaaac ccggtcagag aggaacggca aagccacgca ggagcagaga
    17161 atcagaaaca cggcgcaaaa cagcaggctt tggggcaacg ggatcatgcc gccgaccgcc
    17221 gccagcaatg ccaccgaaag ttcagcgcgt ggcagtgcca gaatccccac gcgcagcgat
    17281 gtcgcgacgc ctgaccgtgt cagcgacgcc ccggccgcgc atccgatcag attggccccc
    17341 aacaccgcca cagccaacag caccgacgtg ccgagcacgg tctcgttcgc cagaagattc
    17401 ggattgagac gcatgcccaa cgaggcgaaa caggccggca tcagcgtcag actcacgaaa
    17461 tcgaggcgct cgcggatctc atgccggaca tccatcgcgg agaaggccac acccatcata
    17521 tacgcgccgg agacgaccgg cagcccgcag gccccgatca acccgcccgc gatcagcaga
    17581 ccggccgcag agaccgcaaa cggctgaccg taattcttct cccgcacggc taacgcgttc
    17641 atgcggcgcg caagcgggat gcccaacaac agcacagccc ccaacccgac acccgcgcgc
    17701 catgccaccg ccgcaatccg cgcaccggaa agctcgttca ctccgcctcc cgcgccgctg
    17761 acacccgcaa ccacggtgaa cacgatcaac ccaagcacat tcccggccat ggccgctgtc
    17821 agcgacaggg tgcccaccgg cgattccaaa cgtttgcgga ccgccagcag acgggccaga
    17881 aagccgacgg atgtgacgga aatcgggaca gcgcaaagca acagcgcgga tgtgtgccac
    17941 ggctgcgccg gacccagcag cgttggcgcg gcccggaaaa gcagcgcgag ccccgcgccg
    18001 aacgcaaaca ggaatccgca cacacccgcc agcaccccgc tcgccgacag gcgccgcatc
    18061 tgacgcaagt ccgtatcgag tccggccaga aaaaagaaga ccgcgaggat cacggccatc
    18121 agaccctgcc cgcccccacc ccacaccgac aggaacgcct ccgcatcccc catcaatccg
    18181 tgcgggaaaa acggcaacgc caccccgccg agcagatacg gccccaccac caccccggcg
    18241 gccacctcgc cgaacaccat cgggagacgc ccccgctcaa acagggcccc tcccagtctg
    18301 gccgcaaaca ccaacacccc gagttgcaga ataaaacgtg ccgcggcatc cgttgctgat
    18361 cccgacgcac cgcaaccgtc agcccagacc ggagacacgg gtatcgcggc caccgccagt
    18421 gccgccgccg cccgcctcat tccgcgtgtg atcattgccg cctccgtccg tggttatgcg
    18481 ccctgccggt tgatccgcgc agcggcaacc cccgcgccga atccgttgtc gatgttcacc
    18541 accgtgaccc cggccgcgca ggagttgagc ataccgagca gcgcggcaag cccttggaaa
    18601 ctcgccccgt acccgacgct cgtcggcacc gcaatcaccg gacggtccgt gagtccgccg
    18661 accacggaag gcagcgcgcc ctccatcccg gccaccacca caatcgcgcg cgcgtcctcg
    18721 aaccggtcga tccgtgagag caaccggtgc aggccggcca ctcccacatc ataaacccgt
    18781 tcgatccgcg cgcccagacg ctcggccgtg agtgccgcct cctccgccac cagaagatcg
    18841 gacgtgccgg cgcagagtac cgccaccttg ccttccggtt gcggcaacgg cgcgacatca
    18901 cgggtcaaga tgtgcgcctc ttcgcggtag acggcatcag ggatctccgt ttgaacagcg
    18961 gcagcctgct ccggcgtcac acgcgtcacg aatgcgtttt gtccggcatc gttcaacgcg
    19021 cgcatgatcg cggcgatctg cacggcagtc ttgcccgatc cgaaaaccac ctcgggcatc
    19081 cccaaccgcc gctggcggtc gaaatccggt tttgcgaagt gaagatcttc agtcatagat
    19141 tacaccaaca aggcatcgtc cagtttggct ttgacctgct cgatcgtaag gcgttcctgc
    19201 agcatggtgt cccggtcgcg gaccgtgacc gtgccgtcct cgatggtctg gaagtcgatg
    19261 gtcacgcacc agggcgtgcc gatctcgtcc tgacggcggt aacggcggcc gatcgcgccg
    19321 ccctcgtccc agaacgcgga gtagcggcgg cgcaggtctt cgaaaatccc gcgggccttg
    19381 ttgtagagat ccggcttgtt cttcacgagc gggaagacgg cgaccttaac cggcgcgatg
    19441 cgcggactga aatgcatcac gatacgggta tcctctttgc ccttatcgtc ggtcaacttc
    19501 tgctcctcgt aggcgttgca gagcagcgcg agcatcatgc ggtccacccc ggcagaaggc
    19561 tcgatgacat gcggcacgta cttgcccaag aagagcttcg acacgaaggc gcgcgcgtcg
    19621 gactcgtcct taccgtgcgc gcgccactcg gcgacgacct tatcggtgaa cgcgagctta
    19681 gcggcgtcat ccagcttatc ggccgcgagc ttgagcggct cgtcaaacac ctccatgctt
    19741 ttgccgctgt gcttttggtg ctgcgagaga tcaaagtccg agcgcgccgc gatcccttcc
    19801 agctcctgca cgccgaaggg gaaccggtat tcgatatcga cacaggcgcg cgcgtagtgg
    19861 gccagctcct caggcttctg ccagtactcg acaaaggacg tcttgggcag tccgcacgcc
    19921 tcgagccaag ccttgcgctg ctcgacccaa tatttgtgcc acacctccca gccccacgag
    19981 tcctgcggca cggagagatc gggatggtcg tccaaggtgg tgacgtgtcc gcaaatcagc
    20041 tcgaccgcct cgtcgggccg gatgaagaac tccagctcca tctgctcgaa ctcgcgcgag
    20101 cggaaggtgt agttgcgcgg cgtgacctcg ttgcggaagg acttgcccat ctgcgcgata
    20161 ccgaacggca cctgctggcg gctcgtggcc agcacattct ggaactgcgc gaaaatcgcc
    20221 tgcgcagtct cgggacgcag ataggcgatg ttcgcggaat cgtccaccgg gccgaccacg
    20281 gtcctgaaca tcaggttgaa cgggcgcggc tcggtcaact caccgccgca gtagggacag
    20341 gggaccaccg caggctgcgg ggccttggca ttcgccagca gcgtgtagag cgcgcgcacc
    20401 gcggcatatg gcgcgggatc ggccttgagc gtctcatagg cggcggtcag cgtgtccgca
    20461 tcgagcacgg ccgccaaatt cggggcaagc cctttggcct tgcctttgca ccagcccgcg
    20521 acttgggccg cgtccgcgcc gccctccagc aacgccttca gactctggcc gatcgcgcct
    20581 tccgccagaa tgtcccacac ctggtcggcg cgcatcagct ttttgcaggc ccggcaggtg
    20641 cacatggggt ccgaaaaggt atcaaggtgt cccgaggctt tccagatcgc cggatgcatg
    20701 atgatcgtgg catcgagtcc cgccacatcc tcgcgattgc gcaccgtggt cttccaccag
    20761 tcctccttga tgttgcgctt catctcgcag cctagcggcc cgtaatccca aaatccgttg
    20821 atccccccgt acacctcgga actgtgaaaa atgaaaccgc ggcgtttaca caatgaggcg
    20881 atgacatcca agctgcttgt gttcgacata tccttccttc acttgcgtgg tcgcgcaatc
    20941 atgcgcttat gaaattgcca ttctcgcccc ccgccggtca gggaccagtg caacaccggc
    21001 ccctgaccgg cgggctcgcg aatcctccag gtgacaaacc agtgttagtt tggcatgcgg
    21061 acggggtgta ggcaagggga aacaggccgt gaacaacgcg cttctccgtc cgatgatccg
    21121 gataatcccg ttattgtccc ttccaacctg ccgtcacact tcatacccca gaggacatga
    21181 gacggcaacc ggtcacgaac gggacacacg atgatgatgc accgcgtgag cggcaaagta
    21241 cggaacgtca ccggccttgg gcaggtcaga taaggcgctt tttaccaccc cgtcgccttt
    21301 gtccgcttgc cagaacgatg gcattttgcg acactgtcgt caaacaacac gttcaccttg
    21361 ggaggcaatc acatgatgcg gatcacacga tatgtcctat gtgcggggat cctttctgcg
    21421 gcctgctgcc ttgcggcaga ggaaggctgg accccgttgt tcaacgggaa agatctggac
    21481 ggctggatcc cgaaaatcaa agggtatccc gcaggcgaga atttcgccaa caccttccgc
    21541 gtggaggacg gcatgatcaa agtgcgctac gacgggtacg acggcgcgtt caaaaaccgt
    21601 ttcgggcacc ttttttacaa aaccccgttc tcccactata ttctgcgtgt cgaataccgg
    21661 tttgtaggag agcagttgcc ggacggtgcc ggatgggcca aacgtaacag cggcgtgatg
    21721 atccacggac agacgccgga aagcatggga gtgaaccagt cgttcccagt atcaatcgag
    21781 gtccagttcc tgggcggact gggcagcggc aaccgcacta ccgccaacct ctgcactccc
    21841 ggcacaaatg ttgaaaagga cggcaaactt tacacgccgc acggactcag cgccaaatcc
    21901 aagacctatc acggcgacca atgggtgacg gttgagatcg aggcgcgcgg tagcgaaacc
    21961 atcctccatc gcatcgaggg cgagacggtc ttgcagtatg acaagccgca gtatgacccc
    22021 aaagacggcg atgcccgacg cctgatcgag gcgaacggcg gcaagctcct gatcgagggc
    22081 gggacaatct cgcttcagtc ggagagccat cccatcgact tccgtaaaat cgagatcaag
    22141 gtgctgaagc cgtagtgtcc ccggcaccat ggcgccgggc cgattcctga gccatcgctg
    22201 actccgcaaa cagcgcttcc acgcgccgca acagttcttc gcgggtttcg acgcgcagca
    22261 atgggcacgg cagactcttg cggaaggtgg cgccgtacga tttggtcacc acacgcggat
    22321 ccgcgatgat gaccacgccc cggtccgccc ggatgcggat caggcggccg aaaccctgcc
    22381 gaaagcggat cacggcctgc ggcagcgaaa actctcggaa agcgcagcct cccgcatctt
    22441 caatctgttc acagcgcgct tcgacaatcg gatcggtgac ggccgcaaag ggcagccgcg
    22501 ccatgaccac gcagctcagc gcctcccccg ccacatcgac cccctcccag aacgaatgcg
    22561 ttccaagcag aacgcagttc ccgccggtac ggaagatccg tgtgatctga tcgcgcgacc
    22621 cgttggtgcc gtgcaccagc agacggatgc cggcctcctg cagcggctcc tccagcagcc
    22681 gcgcgacccg gttcatcatt tcataactgg tgaacagccc catcgcccgc ccgcgtgtcc
    22741 gcgtgaacac gtccaacatc agaccgctga gttgctcggc ataggtgccg cccgtgcccc
    22801 ctgtgggttc gggaagaaaa accggtgcca gcgccgcgca ctgcgtgaga taatcgaacg
    22861 gactcgccgc cacacaggtc aggagccgat ccggttcgag ccggtcgatc cccaatcgcc
    22921 ggctgatata cccgaacgaa tttcccacgc gcaacgtggc cgaacagaac accacggtgg
    22981 ccttccggcg gtaaagcgtc tcggccagcg cttcaccgat acgaagcggt gccgcgtaaa
    23041 gatgggcagt ccccgcactg cggctcggcg gttcggccca aaaaacgtgt tccgcgtcat
    23101 acgcggcgag aacgaaatcc agatccatcg cataggtccg gagcgacgct gccgcgcctt
    23161 cgacattggc agcctgatcg tcatacagcg cgagctcgcc ttccgtcgcc tgccgcaagg
    23221 cttcccccaa ggccagcaac agaccggagg cttcggcgat cgcattcttc aacccgatgc
    23281 gatgcatttg gacagtgtcc tcatcccatg cgtccggaca gggcgtgaac cgcttatcac
    23341 ggaaaacctc gcggcggaaa ccggagggaa acacgccgcc gcatgcgggt tgcccggcat
    23401 ttcccgccgc gagggccgac tcccccgtcg gccaaactgt cgcgtcagga ccgtcgcagg
    23461 gcacacaccg gaaacgcacc ggaccgggtt ccggctggat caagccgtgg agcgactcga
    23521 acagttgccg agccgcagcc tgcacctctt cgagcacatt gcggatctcc cggatttgac
    23581 gccgcagcgc ttgggcgtat tccggatccg cagtcaccgc gcccttctcc aaatggttgc
    23641 gcagaacttc cagcataccc ccggcccgcc gcccccggcc ccggcttagt cggcgcaaca
    23701 gttgggtgag gcgcgtctgc gtaatctccg tggagaggtg gcgcgtcgcg gcctcctcca
    23761 gattgtgggc ctcgtcaaac acgatctggg catgcggcgg cagggcggtc ccgggggccc
    23821 gcgactccgc gaacaccaga gcgtgattcg cgatcaccac atgcgccgca gcggcgcgcg
    23881 cgcgggccct ctgcaaaaaa cagcgccgac tgtagcggca ggcacggcct ggacactctt
    23941 cggcggtaga ggccagcttc gcgaggaact cggcatcgac acgtcccgga cccgcaaagg
    24001 tatcaagatc gccgtccggc gtctgcgcgg cccaggcaag cacatacgcg aaatggcgcc
    24061 attccgggcg atcgagttcg tactggctct gctccagcag aatcgcgagc cgccgcagac
    24121 agaagtagtt tgagcgccct ttaaccaccg ccacgcgcag agcggcgcgc ccgccaggac
    24181 agaccgcctc gcggacccgc ggcaaatcgt tctcgatcag ttgggcctgc aagttccgcg
    24241 tgttggtgct gaccaccacc ggaacatcgt tgagcagcgc ccaggccgcg gccggaatca
    24301 ggtaggccag acttttgccg acacccgtac cggcctcgac caacaaatgg cgcccctcgt
    24361 tgaaggcacg cgtgacggcg tgcagcatct cgacctgtcc ggcacgcggc tcataaccgg
    24421 gcatgagacg tgcgaacgcg ccgccgggaa gcagatgcgc ggccatggcc gcaacatcca
    24481 gcggcgagca gtcggcatgc tcgggcaagg cacggcgatc cccccggcgc gcctccgggg
    24541 cgaaggtctc gatccaaggc ccgcatccga cgccagcttg acgaacgcaa gccgcgaagt
    24601 ggctgaagac gcgcgccagt ccgtgctcat cgagattgcg caggatcccc tcaatcgact
    24661 ccagtgtcca caggggcaac gcgttcaaac gcgccgccac agcctgccac agcaccccga
    24721 tcgcctcacc ggtgtgcggc ccctggcgta tttgcgtggt cacctgctcg gcgggcgtgc
    24781 ccgggaaggc gaaggcgtac agaaccagca catcgaccgc ccacgcccct tcctgcggca
    24841 agaccccctc aaacgcggcc ggatcctgcg tgacgtacac cgtctccccg ccgtcacgtt
    24901 gatccgtgac gggaaagaga tcgaagaccg gcggtcggcc gggatgtcgc gtgacccaaa
    24961 ctgtacggaa gttcagcatg tgcgccgaac cggaaatccg gcctcacttc cccatgtcga
    25021 agagcttcag gcaatgccgg aaatcggcgg gcagcgggct gtgcgcctga agggttttgc
    25081 ccggccgcat cggatgcggc aacccgagtc ccgatgcgtg gagcatcagg cgcggcacac
    25141 tcaacaaacg cgggtcccgc gcaactttca ccccgtattt gcggtccccc agtacgggat
    25201 gacgaatcgc ggccagatga cgccggatct gatgggtacg tcccgtttcg atccgcacct
    25261 ttagaaaact cgcgtcgcgg gagagcatga tccgctgaat gtggctaacc gcgcgctcgc
    25321 cgtccaactc ctgatctacc gttgaggcgt gccgttcgta gcgcccggaa acgatcgtat
    25381 gatactgctt ggtcacccgc cgcgtcttga acaactccac ggcggcgtca aaggcctccg
    25441 gcgtcttggc gcacaacaga cagccggtgg tgtcccggtc gagccggtgc accgccttca
    25501 gttccgcgat ccccgtctgc gcgcgcagcc gctcctccac gctccccgcg ccgaccgtca
    25561 gcagaccgga aggcttgtcc acaaccgcat attccgcatc ctcgatgagc aggcggattt
    25621 tcggcaccgc aacggggttc gcgtcccgcg cgcccttacg cagcaccgca gccggcaact
    25681 ctaccgtatc accctgacgt agcgtatgat gcgccatcca gacacatttg cggttcaccc
    25741 acaccacccg ttcgtccagc agggctttca ccgcccgtcg ggaacgcgag agtttcgcgg
    25801 cgagaaaaac ctgaaccgat tgacctgcct cgctcttgga caccacaaaa gacaacacac
    25861 ccgcccggct catatcaccc gctctttccg cttccgcccc cgttacacct gcagaagcga
    25921 acccaccacc cctgccaccg catcccgtcc caaacggttc ggacccatct gaacctcaag
    25981 atcgatccgc ttgtcttcct ccgcgtctgc caccacccgg caggccaccg gttcgccacc
    26041 cagcgcgcga ttgaaaagct ccggcaacgg ctcgtccggc attagatcct caaacggccg
    26101 tcccaacacc tcgttctctt cccgaagatc gaacatcttc aggaaggccg ggttgacaca
    26161 ccggaacacc cggtcccgat cgcacacaaa acaggcgctc tgcgcgttct caaacgcatt
    26221 cgccatgctg cgcagagcct gccactgttt gcgccggcgc tccgtgttgc ggaccgtgaa
    26281 caccaaatca ccgctattga tcagatcaat cacgctgatg gtcacctccg ccgcgaacac
    26341 cgttccgtcc tgacgctggc acttggcgtc gagcatgatg tggcgcgcct catcgatccc
    26401 tttgcggatc cgcccgatca tctgcggggt gacgcccggg atcagaaaag ccaccggctt
    26461 gtcccacacc tcctccggct taacctgaaa atactcggtc gcacgcggat tgatctcaat
    26521 cacatgcccg ttcgggtccg tgatcagcaa cgcatcatac atgccggcga tgagttgacg
    26581 gtaaagtgat ttatgatccc gcatcgcgca aacggatgtc atgtatcccc cctcttttcc
    26641 aacacctgcc gcacacggtt acatcgtgcg ggacacgttc acaacaacat gattactatg
    26701 acaggttact tcgctttccg caattcctgt tttcatgaaa tatggactcg ccttaccctg
    26761 tccggcgtat tatcatgatc tcaccatttg gggagggagt tatggaagag cggcttgacg
    26821 aaaccacctt cgtttcattc atttgtgagg cgtgcaatca ggagattgag gcacccgcag
    26881 accttgcggg ccgtgaaacc gagtgcccga cctgcggcgt gaccctcacg gtgccttacg
    26941 tctccacgcc cggaaccctc tggggcggtc cccttgaaaa cacggggggc cccactcccg
    27001 cgcagacgga ggccatgaag agccggacga tccgcatcga actgccggac agttggtgac
    27061 cgtcccatgt cccgcatcct gttcatctcc ccgaaaccct actccccttg ggaaggcgcc
    27121 tgccaccgga cgcgccatat actcgaagcc caaattgcct tagggtacga tgtcgacctg
    27181 ctcaccccga cggagggtca gacgcccacc gttgccgact ccgtcaccac tatcccgctg
    27241 ccacgcctcc cgttctgccg acgcacgccc accctccctt cgcttctccg cctcacactg
    27301 gacctgctcg cgcttctcaa gatcctctgg cttgcgggaa acacgcggta tgcgctgatc
    27361 cacggacagg gcaacagcgg ggtcctcgcc tgggccgcgg cgcgcctgct gcgcatcccc
    27421 tgtatcgtgg aactgcggaa cgaccctgcg ccagaccccg cgcaccggcg cggcctcatc
    27481 cccgcactct accgacggct ggaacacaag gcgctgagac aggcggatgc ggtcatcggc
    27541 catgatgcgg caacggtcgc gcgtctgaca gacttgggct gccatagccg cgcgtgcgtg
    27601 atcgccgaca tcccggccgt aaccgaggtg gcggacgcac cggcccgcaa tctggcgaaa
    27661 gcgcgttacc ggaccctgac cgatcaggat ctggtcacct gcgtcgcttc ctatacccgg
    27721 tttcagggac tcgacctctt tttcaacgcg ctcccgcgcc tgttggcaac gctgccccac
    27781 acgcggtgcg tggtcgtcgg cggcggcccg gaggagatcg cgcagatgcg cgaagccctc
    27841 gaccgggcag gcatccatga ggccgtgact ttcccgggcc gattgtccac cagcgagttg
    27901 gccgcgttgc tcgcgatttc ggacgtgctg gtctcgtccc gccgtgccgg cgccaccgca
    27961 cccatcaaag ttctcgacta cctcctttcc ggcactccca tcgtggcagt cgacaccccc
    28021 gccaaccgcg ccatcctctc gccggaaaac gccgtgatca cccccgccac gccggaggga
    28081 ttggcggacg gactcatccg cctcctgagg tcgcccggac tccgtacgga actcagactc
    28141 aaaggccgcg aaaccctgcg ccgcgaaaac cgcacgcccg aggccttccg cgcagccctg
    28201 gcgcgctgtt acacctatgc gctctcccgg tcggaggccg agtgagatga cgcgccgcct
    28261 cctccgtttc gcgcgctcct tcgtcatact ggtctcgctg tttctgctgt cgacattgat
    28321 cgccgccaac gcgctcgttc atctgccgcg ccaacgccaa cagagcctgc tccaaaacct
    28381 tccggatgtt gcggcccaaa cgctcacgcg tgtcggccat gccgcggccg acatcaccga
    28441 cggactcggc tggaccggcc gtgacgccgt ggccgccctg ccgcagccga cggacaccaa
    28501 ccgcctgacc cgcgcgggtg taccgcgccg actgccctcc tgcaccgcgc cggatgatct
    28561 ggtcgtgctc aacaagatcg gcttcaccat cggttattca cccgcgtggc gtcatccggt
    28621 ctgggtcgcc taccgggccg acccgacgtg ccgaaacccg ctgttaccgc gcccctccgg
    28681 attcaagccc gaccctgccg cgccgcgttc gcccaaacac cgggagtact cccgcagcgg
    28741 atacaaccgc ggccatatgg ttcccaaccg cgtgatcgcc tcgcgtcacg gtcccgcagc
    28801 gcaggcgcag accttcctca cctcgaacat ctgcccgcag cgcgcctccc tcaatcaggg
    28861 gccgtgggaa aacctggagt tccgtatcgc ggaactctgg cctacgcggt acggttcggt
    28921 ctgggttctg gccggcgcgg tgcccgcacc cgaggcccgg cgactgccct ccggtatcca
    28981 tgtgcccgcc gcgttttacc agatcgtcgt gacccagccg caggacggga cactccgcgc
    29041 cttcgcggtg tacatgccgc aacacgtccg gcgccgcacc ccggtccgct ccatgctggt
    29101 ttcgatcgac gagttggaga cgctcacggg gttcgatttt ctctccgagc tgccggacga
    29161 ggtggaggca gaactcgaag ccgccacgcc cacgcgcctc tggcccgtgg ggcttgtcgg
    29221 caccatccgg cttgtgcgtg aacgcttccg ccgctacccc gtgaaacgcg ttcagggcgc
    29281 gcgctgatag acgcgcacat aatcgatctc atacgtctga ggcaacatgg cgtcgtcgat
    29341 ccccttttgc ccgccccacg ccccgccgat cgccagattg atgagcaggt agtgcggttt
    29401 acggaacggg ttgtcctccc cggcacccgc cttgtcgagc aagaaccggt gatagagcgt
    29461 atcgtcatag tagaaatcca tgcgatcctc gaaccgctcc acaacataga ggtgaaagtc
    29521 atccgacggt ttctggtcat gcagtttgcc gcccaaagag gtgcgcttgc cttgggtcgt
    29581 gtcaaaccag tgcacattgg cgtgaaccgt tgcgggcgca tgccccacga actccatgat
    29641 gtcgatctcg ccgcaacgcg gccagccgat ggaccggaat gaggtgccga gcatccagat
    29701 cgcgggccac atgccgcgtc cgcgcggcag cttcgcacgc acctcgacac gcccgtatgt
    29761 caccgcgaac aacccctcgg tggtcagact ggccgaagtg taggccgcgg tttccgcctc
    29821 gcgccaatcc tcggatccgg acttgtgccc gggattggca aacgtctcct tgcggccttc
    29881 gatcaccaga cacccgttct cgacccgcgc gttctcccgg cgcgccacgg tgtagaactg
    29941 cgcctcccga ttacggacaa accccttctc atacgtccac ttggccggat ccggcatgcc
    30001 gggccggtca aactcatccg cccacaccag ccgccacccc tcgcgctccg ccgcgcccgc
    30061 accgcatacc agcaaggcga tcacacccaa cacgcatccc atccgcatcc gctccccctt
    30121 tccgccgccc cacagcggca tttcacctca cgttccgaca cccaatctgt tacggcatgc
    30181 cattcacggc caacgccttg ccttgggcag cctcgtcaag ccggggctcc accgcgcgtt
    30241 ctgttatccg ttcacggttc tgccgtcgcg cgtccgatcc ggtccgcatc gtccgcatag
    30301 ctgtacgcga aacgttgccg cgtctccacg caggtcaggg tcggcggcgt ttgctgtccg
    30361 ccggtctggc gcggcagata ctccacggca caggcttgga caggccggtc ccgggaatcg
    30421 gcctgaagca ccttgtgccc cagatacgtg aaccgttccg gcgtcctgcc cggacgcgtg
    30481 cggtaccagc cggtgagacg gccctgatca tcgtatgcgt acagatcctt ccaccctttt
    30541 tcaaaggtga gcatcgggtc ggcgtaccgg tgctccgcat tggtgtagtc aaccgacagg
    30601 acctgtccgt catcccgata ggcccgcacc tcattcggca gataatagaa cgagatgatc
    30661 gaaggcgcgg tatatccctt gccgtgacgc gcgaagcatc cgatatcaac gcggtggcgc
    30721 agcagagccg agggcatccc ttggtcatca accggcctga aggcgccgtg atacgacacc
    30781 gtgagtttca cacgcgaggc gtttgcgttc agaggcgtga tgacaatttt ccgggtgtcg
    30841 ccctgcaaca cggtccagcg gaaggcccct tcctgctgct gaccgggcac tgcggcctcc
    30901 acggtcatca cccgctcgcg ggacacgccg cgcacaaccc gcgcgatgca gcagggcgta
    30961 tcgaacagcc cctcggagcg caggtcgaag aaatccaaac ccgcctgaac cttggcatcc
    31021 tccactgtcc gaagcgccac caagggcggg atcgcctccg gcgtcatggc ctgcgccatc
    31081 cgcaccatgg cctcgacatc cagttgcgcg gcatcgaata caaccggatg cgccgcaccg
    31141 gtcaagtacc gctcctcgtc ctttgcgacc gccttctgcg tgatccggag aatccgctgc
    31201 agcgtcggac cgatccgatt ccgctgcgtc agcgcgcgct tggtcgctgg cgtgaacgcc
    31261 gccaaggttg cggcgaaggc gttcacgaac ggctggtccg aacccgacga tccttggctg
    31321 atcacgaaaa acggggcgtt gaccggatag agatccccgg tttcgggatc atggtcccga
    31381 tgttcaggga acacccaaat ctggtttccg agataaaaac gcagggagat gatcgcccgg
    31441 agcggatcgg tcacggccgc gcgcggcaga ctgcgccaat acgggccact caccatggag
    31501 agtgacgcgt tgcccagtac cggcgcgtcg aacaggccgt tcggcagact caggtggacc
    31561 tgggcctgct ttgcctgctc gccatacacc accggcgtca ggccgggata gttcgacacc
    31621 accagcagcg aatgattccc gtcccggttc acatacagat cgccgaaatt cccggacgcc
    31681 gtccccgcct tgaaccccgc gcgcaccgtt tccgccgccg cgccgcgata cgcctccgca
    31741 taatcggaaa cgggacgcag atccggaacg agcaggttaa accgtgtccg gaaacatccg
    31801 aactcgaaat cccatatcgt gttttccgca ttcacttccg ccggacgtcc cataacaacg
    31861 gatacgggac ggacggacgg cataccttca accggttttc cctgaagacg ccgcgccttg
    31921 tccaacagtt cccggaatgc cttgatgggg ccaagcgatt tcaggtcccg gtccgcctca
    31981 atcgcggcgg cgtcgcggaa accaaggtcg atcgcgcgat ccagcgccgc caacgcttcc
    32041 cctttgtcgg cacggtaagc gagtgcgcag gccagattgt actgccacgt cgggtcacga
    32101 ggcagcaggg ccacgccctc acggcagatc cgctccatct cctcagtctc tcccgtccgc
    32161 acgacccgca tcagttgctg ttgcagagcg aggtgtttcg gatagagcaa cggcatctca
    32221 aacacgttgg tcacggcacc cgcaaccatc gaaatcagac acgcgcagac aaaaatcccg
    32281 cgtttcctca tcacgtcccc cttccctatg ctgtcccgca ctgtcctttt atccaaacag
    32341 cctacgccac cacggcaccg gcgcccgcgt ctgccgcatc agccattgga tgacctcgtc
    32401 attcccgtag gcgcgatccc acacggcatg acccgcacct tcgaattccg tataccggac
    32461 cgcacggttg ccgacctgtt tcagcgcctg aaccatacgc cgggaacagg ccacatcgac
    32521 attctggtcg gccgtgccgt gaaaaaccca gatcggcatc cgccggagtt cccgcacccg
    32581 ccggatatcc cccccgccgc aaacgggaat cgcggcggca aaacgttcgg ggcggcgctg
    32641 aatcgcatcc caagtgccga acccgcccaa cgaaagtccc gtgatgtaga cccggtccgg
    32701 gtccgcctga cacgcatcca ccagatggtc caccatggcc agcacgacct ccatggacgg
    32761 ggcaggctgc ggcggcatgg catagtcttc cccgcgcgcc agccgtttca cccaccagtt
    32821 gccggtttgg cactgcggcg ccaagatcat cacaggttca ggctggcgga gcagggtgga
    32881 cagcagggcg ggcagcccga ctttgaggtg acggcggttg tccgtgccgc actctccgga
    32941 tccgtgcaga aacaggatca ggggatatcg gacgccgggc cggggaaact gcggcgcgct
    33001 caaccggtaa gcgaacgtct ccccggtcgg cgcattggta aaaacgcagg aatgaaaacg
    33061 ttcctcgacc gtgaactgcg cacacgcctt gagggtcagc gcaaaaagaa ccagaagcca
    33121 gcctttcatg gggatcagta tgccataacc cgtccgtctt ggggagcgtc aattcgcgcg
    33181 ctcgtttccc gccggagcgt catgcgcggc accgctttct ggtctgtccg cgccaaagcg
    33241 gcgcgctgat cgatcaactc gcccccagcc ctctgctcat caccacgcgc gccagcgcct
    33301 cgatcgcccc ttccgcggtc gcctccgccg cgaccgcatc cggttgacgg ccgaatccgg
    33361 ccagcgcctc cgaagtcggc ctgccgatcg tcaccacgat ctgaccggcc agcgcctcca
    33421 cgcccgcctg ccccataaac gcctcaaccg ctgatgcgct cgcaaagaaa accgcatcgg
    33481 acgcgggcaa ccggggatag tgaaccggtt cgttgacgta caacagaacg tcctctaccg
    33541 ccgcgccctc ttcgcgcaga acatcggcca acagcggtcc ggccttttcc gaacgcaacc
    33601 gcaacacgtt gcatcccgct atttccaacg tccgcatttc cgccgccaat cccgccgcac
    33661 tgtattccat cggcggcgtc agatccggat ggataccatg gtgccgaaac gccgcggcac
    33721 tccccggccc gcaggccatc acccgcggca gacgccgcag gtcgaccccc gcctgccgga
    33781 tcatgcccat gaacagatga accgccgagg gtgaagtcaa caccacccag tcaaacgacg
    33841 cgatccgcgt caacgcccgc tccgcttcaa cacacggcgt cagccggatc agtggccgga
    33901 gcaacggcac tcccccgaaa tcgaccaccc gcaacgcggt cttgtccagc aacgcctcac
    33961 tgcacgtcaa caacacgcgg caaccacgca acgcgccggt gtcccgccga tacgcgcccg
    34021 ccgccgcttc accgattacc aggagtcccg gcgccttcgt ctccaccggc gcggccgcac
    34081 cctcgcgcaa cccggccagc gtggtgcgga aaatcagttc gtcctccgcc cccgcgtcaa
    34141 aaaccagtcc gaccggggtt ttcccatccc agccctccgc aagcaactgg ccggccatct
    34201 ccgtcgccac acgcacggac atgaacagca ccaacggcag acgccgacgc gcttcgagcg
    34261 acacgctggc tacggcgccg ccctcgaccc gcggcgtgaa tgccgtgaac ccgcgggaca
    34321 cgccgcgttt ggtgagcagc attcctgtgc cggtggtggc cacggtcagc gcactcaccc
    34381 ccgcccgcac gcggtaaggc agttccaacc gttccaactc gtcagtctct tccgccagcc
    34441 gcccgaacaa tccggggtca ccccccttca gccgcacgac ccgtctgccc tgacgggcat
    34501 acttcgcgat gaggtcagta atatcgggct gccgcaccgc atgttcgccg caccgtttac
    34561 ccacgaagac gcgctcggcc gagggcggca gaaactccag caaccggtca tccatcagca
    34621 cgtcgtagag gcatacctcc gcctgccgca aatcccggac gccgccccaa gtgcaggact
    34681 cggccacgcc cacccccgcg cccacaaagc gcaccgcttt gacaaaatat ccgcgcaccc
    34741 tcgtccagac cggatcgccc agtcggaagg tcaccgccaa ccgtccctga ccggcgggca
    34801 ccggcagttc tgaaagcggt atccactcgc tcacgcgctc ttccagaccg agccggttca
    34861 gagccgcgcc agccatcagc agagcgtcat accggccagc atcaagttgc tccaggcgcg
    34921 acgggatcgc gccgcggatc ggcttcagca cggcgtgcgg gaatcgcgcc cgcgcaaagg
    34981 ctccgcgccg ctcgctactg acccccacca ccggcttctc cggcaggtca gcccacgttt
    35041 taccttgcgg cagaacccat gcgtcgcgcg gatcttcccc ccaaggcagc cagaaccagt
    35101 ccaaatccgg cggcacggga tcaggcaggt ctttggcgct gtgtacggcc aaatcgatgc
    35161 atcccttgcg caaggcgtca tccaaatcgc gcgtgaaaaa atccgccgga ctgacgcgca
    35221 ggtcggtggc cagatcccgg tcacccggcg tttccagcgt cacgggatcg aattcgagaa
    35281 acggaaacag cgcggcaatc cggttgagcg cgtcacgcgt ctgagccagc gccagccgac
    35341 tgccccgcgt cccgactttc gcgcgtaccg tttgcatggt taacctttcc ttcacttccg
    35401 aacgagccgt accgcaacat gcccatgagt aaacttcatg ctcttcgtat ccttgtggtg
    35461 taacaacatg ttattgcgtt tgttgatccg taaggcactg ccgaaaactt cgacgttcag
    35521 cgttcgacgt tcagcgttcg acgttcttcc ccggtctcat gttccccctg gacgctgcgc
    35581 cgcatgcgct catagaggct gcggtgctcg cgcaggatcg cgtcacatgc tttcagcact
    35641 ttgtccaatg tgccgttggc ggaccggtac caccgtttga tctcatccag atccgccacg
    35701 cgtaccccgt tgccgaacgt gtccagcgcc ggatcgatat tccggggcat gccgaggtcg
    35761 atcagcgtca caccgcgcgg attcaaccgg gcaccatgct ccaaccggtt cagcaccggc
    35821 tcggatgcat tgaccgcact caccacagcg tcaatcgtgg gcagcacacg cgccaggtct
    35881 ttcagctgga tcacttcgat ccggtgcgcg tcttgggcgc tcgacgcggg cgcgttgcga
    35941 tgataggccc acacgcaatg gcagccgcgg cgcagcagat cctccatcag cccctgcccg
    36001 accacccccg tgccgatcac cagcacacgg ctgtccggca gcgccggcag ctgttccgcc
    36061 aagtaccgca acgccaaatc ctcgatctcc gcgacgtcca gcagcggctc cacctcatga
    36121 cggatatcct tggaaatgtg cagcgccgca tcgcacaact cccggatgac cgatcccgcc
    36181 caaccgaacg tgacggcctc atccaccgcc tccttcacct gagagaccac atggaactcg
    36241 cccggcgtct gcgaatccat gccagccgcg accaggcaca ggtgcgcgaa cgcgtcaaac
    36301 ccgcgcttca tgtaaaaccg gtccggcggc aattggtcga accgcagcaa cctccgcaaa
    36361 atcccgctgg ttcccgcctc gcgcgaaatc gcggccacca cttcaatacg gttgcaggtg
    36421 ttgagaatga aaaactcatg cacaccccag atctgccgga tcatcccgcc aatcgcctcg
    36481 cgttcccgca acagcaaatg gaatggcgca cgttcctcag ccgaaaggta ctcatgactg
    36541 gtacccagca ccagcagatc cgaattttcg atcgaatcca gatgtttccg aagattttct
    36601 ttcaccagcc gcgattgccg acaggatttg ccgctggtgg agacggcgac cagcacatcc
    36661 ccggtgcgga taatagcggg cgtcacaaaa tccccgtccg gccaattccc gtccgcgcag
    36721 cagcacagaa tccgattggc ttgtgcgctt tcgagaacgg ttcggttgac gcgcttgtca
    36781 tccgtgcagg cgaagaccat cgccgcgccc tccacatcgg ccggctcaaa ccggcgagcc
    36841 gtatggtgga tcgccccggt ttcggccagg gtttgcaatt ccgccacaca gtccggacac
    36901 acgagccgaa cctcggcgcc cgaatccaac aaaagctcga ctttgcgctg cccgacgcgt
    36961 ccgcccccca caaccaacac gcggcgacgc tcaatcatcg cgttgatcgg aaatgtcttt
    37021 cgaaccggtc ttgtgtctcg catagatcag aaaaaccgta tcacattcct gagcgcttga
    37081 ccacacacga gaccgggaag acccctttag tcgatcgcgt cacccagctc catgttgtcg
    37141 agatcgatct ccccgctgaa cacaaacttg accggaccag acagcgtcaa tccggtacat
    37201 ttgcgatgac gccagtcgcc atccaccgtg aggtcgtatc ccgaggatgt cttcactttg
    37261 gcgggcagcg tgaaccctgc ggtctcgacc gccaccacca cgcaggcgac ggccccggtt
    37321 ccgcaggcac cggattccgc ttcgaccccc cgctcgtagg tgcgcatcgt catccggttg
    37381 ggtgcgcgca acgtgacaaa atccacattc gttccgtccg gcgcgaaaac gggatggcga
    37441 cgcagcgcgc gcccgagacc ctgcacatcc accgcgctga tatgaggcac ctgcaccaca
    37501 aaatgcggca cgcccgtatt cagaaaatcc ccgcgaatcg tctccccgtc aatctggacc
    37561 tcgatcccgt agtttttgcg ggtcggctcc ggcatccaca cgcaaacccc gtttttggtg
    37621 agctgcgcgt ccaccaggcc gcacatcgtc tccatggtga gggctcggcc gctggcaccg
    37681 atggcatgcg cgaacgccgc cgcgcaccgg gaaccgttgc cgcacatctc cacctcggtg
    37741 ccgtccgggt tgaggaaccg catccggaag tcggctttat ccgaacgctg aagcagaatc
    37801 accccttcac aaccgattcc ggtgtgccgt cccgccaagg cggccatcag aaaatgatcc
    37861 tcccacggaa ccgttccctc ccggtcatcg atcagcacaa aatcgtttcc cgcgccgtgc
    37921 attttcgtga aacggatttt cattcagtcc ctcctccggc ttgtccgcgt tacggcaact
    37981 cccgtccgaa aatgcgcatc atgcgcgtcc gtaaatcgcc ctccgtctcc atcgggatct
    38041 cgccgccgcc cgacaacgcg accagatcgg cgaggatatg catcgcctca tccagcacca
    38101 catcgtcttc ccattcctcc tgctccgaag catccgacgc ctcatcctca attttccgca
    38161 tctcgcgttc agcgcgcatc atcgcgcggc gcgcctccca ctccaacggc acctcggtct
    38221 tctcgttcaa ttcctgcaca tgccggacca gagtgcaata ccgtgcgtac cggctgttgt
    38281 ccgccaggcg ttgggctgag aaagccttca actgcggcac aaacgccgcc acatccgaaa
    38341 ccggaatgta gagtgcggat tcaacccggc tccagggcag ggcacccggc atcttgtctt
    38401 ccccgatgtc cagcccgtcg agcgtcgacg gaatgacgat atccgccgcc acaccccggc
    38461 gttgcgtgga tgcgccgttg atgcggtaaa aactcgcggt ggtcaccttc atggatccca
    38521 ttttctccga acctaaaggc atcaccgtct gcacggtgcc tttgccgtgc gattgcgtat
    38581 caccaaccaa caccgcgcgg ccgtaatcct gcagcgcgcc cgccacaatt tcggaagccg
    38641 acgcgctggc gcggttgatc agcaccacca ccggcttgcg gaacgccatg gccggatcga
    38701 gattggggac agggagcacc accaactgac gggcctcgcg cacctgaacc gcgggaccgc
    38761 tccgcacaaa caggccggtc aggctgatcg cctcgcgcag cgacccgccc ccgttgttcc
    38821 gcaaatccag aatcagcccc gcgacctgtt cgctgttgaa cgcggcgagt tggcgggcga
    38881 cgtccagcgt cgcgctgcgg aaccccgcct gacccgggcg tttgtccatt gtaccgtaaa
    38941 acgtgggcag ccgaaccacc ccgaccttga cggtcttgcc ctcgggggag gtcacccgcg
    39001 cgacacggcc ggtcgccgcc tgctcctcga gtttcacctc atcccggatc aggtcaatgc
    39061 gcttggtgat cgtcccgcta ggctccgaag cgggtatgac ctccaacacc acccgggtcc
    39121 ccttctcgcc gcgaatctta cgtaccgctt tgttaagcgg cagatgcacg atatcctcga
    39181 tcggtccgtt gccctgcccc acaccgatga tcttatcccc cttcgtcagg cgctgggcgc
    39241 gcttgtcccg gtcggcgggc ccgccgggga tgatctccac caccaacgcg gcgccgtctt
    39301 cggaccggag ttgcgcgccg atcccgcaga gcgagagatt catgtcaatg tcaaaatcct
    39361 ctttacgcat gggcgacatg taatccgagt gcgggtcata cgccatggcc acggcactga
    39421 ggtaacgctg caacaccgcc tcctcgtcca tatcctgcac gacaatcatc aactgcttgt
    39481 agcgcttggc gatgttctgc tcgggcgtat ccgttaccgt actctccgca cccggcatcg
    39541 cgttcgtaac ggcctgcacc ggggcattgg tcacaccgag ccggtccagt tcgcggctga
    39601 tgcgctgcga cagcacctcg tttttgatgc gtttgcgcca caaatcatcc tgctcggtca
    39661 aagtcgcggg ccaaggcgcc tcccgccgtt tccaggtgta gctttcctcc tgatcaaagg
    39721 tgaacccttg ctcaagcagg ttggtgacgg cctgatagcg ctccgtgacc cgcttgcgga
    39781 aaacccggta cacctcaaag gggaacgaga catcccccgc cttcaacgca tcatcgatct
    39841 gggtccgcat cggaatgaag gcgtcgatat cgctctgcag aaagtagctg tggtcgaaat
    39901 cgaacgcgtt aagcaggttg gtccaggcga cctgagaaat ggcgtcattg aggggtttct
    39961 gcgaaagatg ggcagctggc agcacccgcg cgactttctg cgcgacgccg ccataatggg
    40021 gctgcggcac cagcgtagtc tgtgccgcgc ccgctatccg cggcatcatc ccgagcacga
    40081 agagcgccaa cccgaaaaac cggcatgtcc tcatcatacg tctttctgct ccaatacctt
    40141 cacggcccgt tcgatctcat ctgcggacag atgcccgccc cgttcccgca tcacccccgc
    40201 aaggccgctc acattgcaga ccacgcaccc ttcacacacc gaaacccccg ggcggaaggt
    40261 gttgctggcg aaacagacaa ccggcacaca ggccggtgac ccgcccaccc gctcggccaa
    40321 aaaagatgtc actgccgctg ccgaggcccg tgcctgctcc aacggtggcc tgctcggcaa
    40381 accgccatcc acccgcaaca cgccctcctc cagcgtgacg gaaccagacc agcacttcgt
    40441 ttcgatcgca aacaggccgg tcggccccac caccacatgg tcgatcccct ccgaacgccc
    40501 gccgcaaaga agatcatgaa acacatgaaa accgcgcggc agaccggaaa gcacccaggc
    40561 cacccgctct tccccacggg cccctttgaa aaaagcgtcc agtccggcaa gcccgtcacg
    40621 caccgcccat accagcgccg ccgcggtcac aagcaggccg agccccgccg cctcccacgt
    40681 gatccgcggc agcagcagcc ccgaaaacag accgagaacg aacagggccg ccagcagcgg
    40741 gccgaccgcc cgcatcaccc cggccgcacg ggcgcgttcg cccggaacac cgtatatccg
    40801 cgctgactcc ggctcttgca taatgggttc aattattgtg accgattcac acaccggttg
    40861 caaccctctt cttgccggcg atctgccggg ccatgttttc ggcggtcagt ccgcatccct
    40921 ccagcaactc ggcgaggttc ccttgtccca caaaccggtc gggccagcca aaggccgaca
    40981 caggacaatc aaaaccgcca gccgccagag cctcgcgcaa agccgagccg aacccgccgc
    41041 tgaccgcgcc gttctccatt gtcacgaatt gcgccgcgtt tccggcctga gcatagagca
    41101 gcgtcgtatc catcggtttc acgaaccggg cattcaccac gccggaacgc aggcccgcgt
    41161 cccgcagacg ggtcgccacg tcgcgcgcca acggcagcat gtcgcccagt gcccaaatcc
    41221 acacctcgcc cccctccggc cccgcatccg gatgaagcaa gacttccgcc tgtccgacgg
    41281 acaatgccgg gaccgccgcg tcacacgcca cctccggacc cggttcacgc ggataccgga
    41341 tcaccgacgg cccgtcgagc gcgagggctg tccgcagcat ggcctccagt tccacccgat
    41401 ccttgggctg catgatcgtc agattcggca gacagcgcaa catggggata tcgtagaccc
    41461 cgtgatgcgt gggtccgtcg ccccccacca cgccggcccg gtccacgcag aacaccaccg
    41521 gcaggcgcgg gatgcaaacg tcctgcatca cacagtcgac cgcccgctgc aagaacgtgg
    41581 aatataccgc aaacaccggg cgcaagccgg cggtggccaa acctgcggcg aacaccaccg
    41641 cgtgcgcctc acagattccc acatcgaaga atcgttccgg atggcgcgac gcgaacggct
    41701 ccaaccccgt gccgctacac atggcggcgg tgatcgccac aaccgacggg tcccgttccg
    41761 ccagagcggt cagcgtttcg ccgaacgcct gcgaatatcc gcccggacgc gcctcaggcg
    41821 gttggccggc ctccagatca aacgcaccca cgccatgcca ggcgctggga cggcgttccg
    41881 cgggccgaaa cccccgcccc ttttgtgtgg cgacatggat cagtacggaa cgcttgtatt
    41941 cgcgcgccga acagagggca ttctccaacg cgcggaaatc gtgtccgtcg atcggcccca
    42001 cgtaccgcag gccaaaggcc tcgaagaaag cgttctgcag ccacaggctc ttgatcgcct
    42061 gttccagccg gtagtaaatc cgacgcagcg gtgtcatgcg caaccggtgg ccggccgctt
    42121 cggcagcggc cttgatgcgg ttgtagcgca catccgcgag catccgaccg agacggcggg
    42181 agagcgcgcc cacattctcg gagatcgaca tctcattgtc attgaggatg acgatcacct
    42241 tgcttcgcac ctcctcgatg ctgttgagcg cctcgagcgc gatcccattg gccatggcgc
    42301 cgtcgccgat caccgcgacc acatgccccg cgccgttccc ccgctcacgc cctgccgcca
    42361 atcccagagc ggatgaaagc gcggtcccgg catgaccggc cacgcatacg tcacagtcac
    42421 tctcttccgg ccgggtgaac cccgaaagcc cgtcccgctg ccgcagggtg tgaaaacgct
    42481 cccgccgccc cgtaagcagt ttccaggcgt aggtttggtg gccgacatcc caaatcacct
    42541 tgtcctgcgg cggagaaaag gcgcgcagaa tcgcgatggt cagttcgacc gcaccgagat
    42601 tggaggccaa atgaccgccg ttccggctta ccgtctccag aatcgcctcc cggatctctt
    42661 cggccaggac cggcaactgt ccagattcga gaacctgcac atcagcggga gtccggatcg
    42721 ttttcaacaa attcgacata acctcactct gcggctttct ccgcggccga ggtccgacgc
    42781 ttccagatct gaagcgtcgt gccgttctgc ccgaagccgg gaacctcctg cacacgatcg
    42841 tagtatttgc cgaccttact gaagaacatc tgccgctcgg cgtgcggcac ctcatccatc
    42901 accggcgcac gcacggctaa cccatatccg ctgaaggccg ccacccggca cgacgcggac
    42961 gtgatcagtt cgctcaaacg ccgggtattc atcacatgaa accgctgcgc gtcctcgtct
    43021 cccagttccg gaaaataact gaacggcccc atttccagcc catccggcac acgcatcccg
    43081 gcctccaccg caagataggt gtcctgtgtc agcaacagcc ggtcctcctc agccagcgcc
    43141 cgcacgtcac gggccgtcat ccggagcagc gccagatcag gcatcggctt acgaaccacc
    43201 cagaaccggt cctgcccgtg cacgaaccac tcctgcgcca gcggtgaggc aaaggccgcg
    43261 agcagcgagg ccacggtcca gaacaggcaa agacaaccgc gcaacgttcc ggaggaggtg
    43321 ctgttcgcga cccaccccgc cagcgcagca gcgagcaacc ccatcaccgg aacctgatag
    43381 tcgtcatacg ggaaggggct gagcagatgc accagaaaga ccgcgagaaa accgagtgtc
    43441 cagattgcgg gccacatccc cccctgatac cgaacgcccg ccggttcttc ttgcgcgttc
    43501 tgtcccacta tgccgctgcg gaagatccgg aagatgatca gcgccgcagt caacagcgcc
    43561 atcggcaaat aggcgcggac tgtccgggag aatgacccgg ccagatagaa ccaatcccgg
    43621 ccgccgcgcg agacatgata cgtctggcca aagataaacg cttcgcgcgc ctccatcaga
    43681 cagggtccgt acaccagcgc cagccccact cccccaccca atgcgaacca gaaaaacgcg
    43741 gctccggtct tacggcaggt gaccagcaac gccagcgcgg taaccggcag cagcgcgccc
    43801 agtgaaagcc gtgtgcccgc cgcaaacgcg atcaggagac cggcaggcag cgcccacatg
    43861 caggagaggg cggaacgggt cttacagcga cgtgacacgc acagcgacag aaccccgtat
    43921 ccgcccagaa gcagacagga ggccagcgca taggttttgg ggatcgtagt gaaatagatg
    43981 tgatacagat tgcatcccgt caacgcgaac gcgatcaggc cggcttccgc cgcccgctgg
    44041 gccggcaccg cgcgccgcgc caacccggcc gccatgacac accccgccaa ccccatcagt
    44101 gccgtcagaa tccttccgcc caaaacccca aaaggtgtcc agaggcccga gaagagcgca
    44161 tacacgtacg gcagaagcgg cccctgcgtg aaaaagaaat cccggtacgg tctggccccc
    44221 tcggccaccc ggatcgccgc atacaggtac cacccctcat cctgattgag gttgccgaac
    44281 catacggcga aagcgtacaa cacaaccgta gcccccgcca acagagtcgc ggcacaccct
    44341 aaacaaaagc gatgactccc ttttcggagc atgtccctcc ccccaaaaat tacccgccgc
    44401 gatgccgcat cctgtccgct ctcattgcgc ccttagtata acccgatttc tttccgtcac
    44461 gcaagcagac agccggatca ccgcttgacc cgaggccccg cttccgcata ctatccccgc
    44521 atccggaaaa gacaccaaag gcggaaaagc aggcgccgcc accagcccgg tgaaccgccg
    44581 cggggcgtga accccgcacg catgtactcg cgatctgaaa gtcccatgaa tgcgtttcct
    44641 gactctgtca ctccaccgcc cgcagccctt ccccgcgaca cgccgggcga acggctgcag
    44701 aacgtcctcg cgcaccgcgg cgtcgcatcg cgccgccatg ccgccgacat catcgcaaac
    44761 ggctcggtca ccgtcaacgg caaggtgatc ttcgcgcccg gattccgcgt cgatccgacc
    44821 cgcgatgaca tccgcgtcaa cggggagcgt cttgcgaaga cagaggaacg ttcccgcacg
    44881 atcctgctcc ataaacccgc aggcctgatt tgcagcgcgg actcttcgcg tggccaaacg
    44941 gtctgcgacc tgatgcgccc gcatttccgc gaacggatcg tgcccgtggg gcggctcgac
    45001 aaagagagtg aagggctgct gctgctttcc aatgacggcg aactcatcca ccgtatgacg
    45061 catccgcgat acggtcactc caaaacctac ctcgtccggg tggcgggccg catggccgct
    45121 tccaaaatcg aactgctgcg ctcgcgtttg gagatcgacg ggtacaccat ccggccggtg
    45181 aaggtcgagg tgctgaagga gggacgcgac aacacgcatc ttctggcctt caccctgagc
    45241 gaaggccgaa accgccagat ccgcaaaatg tgctctctgg cgcatttcgt cgtgctgaac
    45301 ctcacccgcg tacgcatcgg cccgctgaag atcggcacga tgcgccccgg cgaatggcgg
    45361 gaactctcgg aagccgaagt ccgggcactt aagcgcacgc cttccgcatc cgtcatcccg
    45421 ccgccggatc gcggcggccc gacccggggc ggcacgccgc cccacgccag gccgaaaccg
    45481 gacctcaggc cccctggccc gcgaaaaagc ggtccatcag cgcgctgtgc tcggcatccg
    45541 tgaacccgcg gctctcgatc gtgacccagc cgttgtagcc gatctcatcg atcaccttgc
    45601 gcacggagcg ccagtccacg ctgccttttc cgatcggcac gaaatccccc tcgttcttct
    45661 tttcgcggtc gattttgaaa tccttgatgt gaagtttcac gatctgggaa cccaacgcgc
    45721 gcagccactc ctcggtcggg gcgtaccgca cgttgttgcc gaggtcgaag taggctttca
    45781 cccacgggtt gtcaaacgac cggacaaacg cggccgcgaa atccggcagc acccagagat
    45841 tgttccacac attctccagc gcgatgatca ccccttcgcg cgccgcgaca ggaatcagtt
    45901 cctcaatcgc gcgccgcgac atctcggtcg cctgattctg cgcggcgatg tactcggcaa
    45961 acggcgcgtt gtccccctcg accaccgacg tgacgcgcag cgtccgggga tcaaacgcaa
    46021 tccggaactg cgacggcttg ggcatggtgc cgcccaccct gcacggcacc agcaggataa
    46081 cggaggatcc atacgttgcc gtgaggcgga tcatcctctt gacgtcctcg atcgctttgc
    46141 gccgctgggc ctcgtccgag ctgttgaaat tcgtccaccc gcccatgaaa gaatggatgg
    46201 cgagtccgtg tttcgcggcc gtcaaacgtc cttggatcgc ctgttcctcg gtcacgttct
    46261 tattggacag ttcgacaccg ggaaagccgg ccttggcgat ccgctcacag gtcttgtcat
    46321 ccgccacacc cgcaattaac gccttgtaca gcgtggtccg gaacgcgcat gcgccccccc
    46381 cctcctgcgc gaccccgaaa cgcggcatcc ccgtcactgc cccggctgcc gccacaccca
    46441 tcatgaatgc cctgcggttc atctgcatgt ccatcctccc cttgcctttc ctgtgctccc
    46501 gttccgttgg gctgcacacc cttttccgga tttccagtcc catcgcatca ggcgcatgcg
    46561 cctgttccca atttgacgta aacagtatag agaccccgga acgtctatgt caagacacac
    46621 gcggtcagga cagatcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagg
    46741 gggtttcgga gttgactgcg ttcgcctccc gcctgcataa tgcgtttcaa ccacaagacc
    46801 ggcacggcac ctgtaaaggc tggggaacgc cggcggctcg aataatctct tgccgagcat
    46861 gacccaactg atctgttggc aatgcccaaa atgggggtaa gcgggatgaa tcgaaatgcg
    46921 ggaatgttgt tggcgatcgt gctttcattg caagtcaggg cagcgggtga tccggcagcg
    46981 gtagcggcgt gggaaaagtc gggacgcgcg gaaacactcg aatggttcaa acgccattgg
    47041 ttcggcgtgc gcccggcgga acgtttggcg gatcaagaga tcggcgagga ttacgtcgcg
    47101 tgccacggcg ggcagatcag gatcaacatt tacgtgaagc ttccggaagg cgcgagtgcc
    47161 gagaacccgg tgccggcttt catcttcggc gaccactaca atcccaagac aagcagaacc
    47221 aagacagaca cgatgccggg aatcccgacg aacgcgatcg tctcgcgcgg ctacgcctac
    47281 atccggttca atttcaatga cgtgacgccc aacaccaacc aagggtgcca cgacccgtca
    47341 agcgccaaca tgaaactcgg cggaatgggc aatcccgaca gctggggcac gatctcggct
    47401 tgggcgtggg gtttctctcg cgtaatggac tgggtggaga cacggccgga actggatgcg
    47461 aaacgcatcg cggtaatcgg acactcccgc ggcggcaaga ccgcgctatg ggccggatgc
    47521 actgaccaac gcatcgcgct cacgatttcg aactgttccg gtaccggcgg ggcgcgttat
    47581 ttgcatatgc cgctcaaagg aatggaaacg ctggactgga tgaaagacca tgcgacgcgg
    47641 cactggttct gtccgagctg gataccgacc tatcgcaacc gcgagacgga aatcgagcat
    47701 gatgcggacg aaatgatcat gctcatcgcg ccgcggcgag tctacgtggc aagcgggtcg
    47761 gaagatagct gggcaggtcc cgcaggcgag ttcgaatcgg ccaaacgcgc gtcggcgctc
    47821 tgggaagcct atggacacaa ggggctttcg ctcgacacgt tcccggtgcc gggtacggtc
    47881 gaccactccg gccgggtcgg ataccatttg cgtccgggag cgcacgcgat caggccgtgg
    47941 gactgggacc gctacatgga cttcatggac aaacatctgc cggggccgaa aaaggcaccc
    48001 ccgaaagcga tggagcccgc ggcaccggaa tcgcaacgtg tccaatcaga ggccatcctc
    48061 gcgtggatca acgccttgga agcctttttc gacggcggcg agcaaggctc tgttcacggc
    48121 ttttcgatcc tccggcacgg caaagtgatt gccgagggca gttggaaacc gttcgacacg
    48181 ctgcatgaga cacacatgct ctattcgctc tcgaaaagct ttgcctcgac cgcgatcgga
    48241 ttgctggtgg atgacggcaa gctggacttg gacgaacgga tcactgacct ctttccggaa
    48301 tacgttccgg cggaaccatc cgaaaacctg aaggcgatgc gtgtgcgcga tctgctctcc
    48361 atgaatgtcg gcacgagcca tgccgatccg cacaagaaag accccggcgg cgattgggtg
    48421 aagatgttcg tcaccaacgt gatcgacggc aagcccggcc aggctttcag gtacgacagc
    48481 agcgccaccc atgtgctggt cgcgatcgcc gagaagagaa gcggcaggac gtttttggat
    48541 ttcatcgggg agcggctctt cgaccggatc ggcatcggga agaatgcctg gaccacttat
    48601 tcgccgaccg gtatcgcttg cggcggatgg gggctcaatc tgacaacccg cgacctggcg
    48661 cggtttggac aactttatct gcagcgcggt gtctggaacg gcgaaagggt cttgtcgcga
    48721 gagtgggtag aactcgcgac agcccggcac acctggaacg gcgggattgt cggcacgcgt
    48781 ccggatgacg actgggcgca ggggtacggc ttccagttct ggcgctgtgt tcccgagggc
    48841 gtttaccgtg cggacggggc ttatggccaa tacacgattg tgttgcccga tcaagatatg
    48901 gtggtgtcaa tgcacgccgg ggtcaaaagc atgggcaaaa tattaaagct gacctgggaa
    48961 caccttttgc ccggagtcag gaacgtcccg ttgccggtga acacggtcgg tgaggaagcg
    49021 ttgcagaaac gtcttggcgg tctcgccatt gccccggtaa aaggctcggt cgcggaaggc
    49081 gcgtatttcg gccgggaact cgctctcaag gaaaacaatc gcggcatcag gtcaatccgc
    49141 ctcgaccgcg aaggcgaagg atggaagtgt acgctcgtga cccgcgccgg ggaaaacgcg
    49201 ttcccggtcg gatgcggcgt ttgggcaaaa ggcacgatca ggatcgatac ggagaaatac
    49261 gaaaagcctg gcggacttat cggagtgcag ccgacggtgg cttcgggtgg cgtggatgcg
    49321 gacggcgtct tccgcatgaa ggttttcctg accggaacaa ccgcttacct ttcgttcgaa
    49381 atctcagaaa agcagattct gggccgactt tttgccatag acggatgccg gtttgagggg
    49441 aagacggaat aaattggttt cagg
//
LOCUS       DAOSLE010000003        47706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_880_length_47706_cov_7.315838, whole genome shotgun sequence.
ACCESSION   DAOSLE010000003 DAOSLE010000000
VERSION     DAOSLE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 47706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47706
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_880_length_47706_cov_7.315838"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..604)
                     /locus_tag="PK576_00330"
     CDS             complement(137..604)
                     /locus_tag="PK576_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOE60068.1"
                     /translation="MSSTSARIDRSLELFHAGCACSQAVFAAYSDLFGIDEACAMRLS
                     CGLGAGVGRLRNVCGAVSAMALLAGLKYGSETPGDAEAKRKTYEVVRQMASAFQARHG
                     SIVCRELLGTIRSETSAVPSERTTAYYATRPCARLVADAASIIEATLLVPDAP"
     gene            873..2132
                     /gene="nusA"
                     /locus_tag="PK576_00335"
     CDS             873..2132
                     /gene="nusA"
                     /locus_tag="PK576_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOE60069.1"
                     /translation="MNNELLSIINYLERDRGVNREIIIQAIESAIQQAARKTSDSTYE
                     LRVEVDRKTLAIKAYDTLTVSNDETGPGFISLRRAQRLRPGAKVGETVEVAVPPAQLG
                     RIAAQTARQMILQKIREAERKNMYDEYKDRIGDIVNGMVRQISHRDLIVELGKTEAIL
                     PAKERLPTEDYNIGDRIRAYVYRVQTGVNGPSVVLSRACPDFVKTLFRLEVAEIADGI
                     VEVMGVARDPGYRSKIAVKTLDEKVDPVGACVGLRGVRVRNIVRELNGEKIDIVRWSD
                     DIKQYVAQALAPARLESVSIDANELRTVNVMVAPDQLSLAIGKRGQNVRLTSKLTGWR
                     VNIIKSGEEVTFEEQRETAIASLAEVLDITTEIATTLVANGFLNTDGIIEAEIPYLQE
                     VTGLDEETVQKIWAAAQAVSGGEDREE"
     gene            2142..4709
                     /gene="infB"
                     /locus_tag="PK576_00340"
     CDS             2142..4709
                     /gene="infB"
                     /locus_tag="PK576_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOE60070.1"
                     /translation="MRVFELAKSMQTSSVEVLKQADALGIEAYSPLSALEDQEVSRLQ
                     AYFAGLGAEVKERASAERAERARAKQERARSRRMAQSQADRAVLEANRARALEMDVRA
                     RGEVSPEPAAPAPAAEAETTPPSTAAPEKPAPAPQKKTTPATAATARPTSAVKPTAAK
                     PAAAKPTAQKTKPARYASAEDELEPEEQLAASFMQRIAKEPEREMSKPPRMTPEGKTR
                     EKEKEIGRIPQRPASAASAAARPGSRPSRFTPVSQQRMESGTPPDRIILLRGAVVVKE
                     LAEKLGIRANRLIADLMQLNILASINQRVEVDVAQKVADKYGFKVEIEKAKRSTERRP
                     VLRSEDADDTIPDDKPEDLLPRPPVVTFLGHVDHGKTSLMDRIRNTRVTSGEAGGITQ
                     HIGAYTVEINGRKITFLDTPGHAAFSAMRARGASLTDIAVIIIAADDGIMPQTREAIR
                     HAQQAGVQIMIAINKCDLPTAKPDRVRQMLQSEGLTPEEWGGDIISVEVSAMTGKGVD
                     HLLEMILLQADMLELSANPARRADGFVVEAQLEQGLGPTATLLIAGGTLNVGDAILCG
                     EHFGKIRGLIDDRGRRVKSAGPATAVKCMGLSGVPEAGAAFRVMLNEKRARELAEKLA
                     QERKEIELSSAKATSLDALMSQMQENQRRELALIVKADTQGSSEAVCEALREIKSEKV
                     TLNIIHEAIGNVSATDVNKAAAGRALIVGFSVGCDAGVQQLARHAGVRINTFRIIYEL
                     LEFVKQRMLDLLPPEYKEVIRGHAEIRAIFDIGKTGRVAGCQMLDGVIRADARCRIFR
                     KKEQIWDGRFAALKHFQDDVSEVTGSQECGIFFDGFEAFQEGDTVECYVLEELPRSL"
     gene            4737..5132
                     /gene="rbfA"
                     /locus_tag="PK576_00345"
     CDS             4737..5132
                     /gene="rbfA"
                     /locus_tag="PK576_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOE60071.1"
                     /translation="MAVDRVERLNALLRREIGEALYKVFASDPLDLAAITVTKVRVAR
                     NLRNATVQVSIFGHESERGEFIRKLAGKAKELQALINRNLNLKYTPRLRFELDGSLEK
                     GDRVLDVLSRLEGLETHGETNPAPDVPPG"
     gene            5155..5895
                     /gene="truB"
                     /locus_tag="PK576_00350"
     CDS             5155..5895
                     /gene="truB"
                     /locus_tag="PK576_00350"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOE60072.1"
                     /translation="MTRYSNAPSSLTPFDGLLLVDKPSGYTSHDLVRLIRKTFRVEKV
                     GHGGTLDPGATGLLLILLGKGTKCSDQVMAGDKTYSAVIRLGRTTSTQDREGETLEEK
                     PWDTVTREAFEAKLTEFTGDLFQTPPMVSAIKVNGVPLYKLARKGQEVERKPRFVHVY
                     RFNLTEWTPPLAHVEVVCTKGTYVRTLAHDVGQALGCGACLDALRRTASGPFRVEDAL
                     PLDAVLRLTPALLAERVIPLYRLAGRRA"
     gene            5892..6893
                     /gene="ribF"
                     /locus_tag="PK576_00355"
     CDS             5892..6893
                     /gene="ribF"
                     /locus_tag="PK576_00355"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOE60073.1"
                     /translation="MRILRQAVSFRRIPGPVFLAVGFFDGVHKGHQLVLGGTVERARE
                     VGGQAWVLTFDRHPMAVLAPSKCPPLLCTLEERLHRLELSGVDGVLLLPFTRKLAVRK
                     PETFIRWLCGAPPQTGTGQNAPSPGTRLREIRCGDNWRFGRRAAGTPEELARYGGIYG
                     YRVVIVPYARYQGVEISSTRIRYAVREGRLKDAAAMLGRPFSIRDVVVRGRGLGRDRC
                     VATANLHPRSDVLPPNGVYAVRVLTGDAAYNGVANLGMHPTFTDVRPERPVLETHLLG
                     FEGDLYGQTIEVVFLERLRDECVFDSAEALASQIAEDIRNATLIFQQHGTPGPQRSP"
     gene            complement(6885..7667)
                     /locus_tag="PK576_00360"
     CDS             complement(6885..7667)
                     /locus_tag="PK576_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOE60074.1"
                     /translation="MSTQKPLIRVGLFGFGRTGAVVAAEMVDSPRCTLSWVVRKSTSR
                     MGEYVSRLHGVDSDEGMIHWIGNVNDEFFVNNPVDVIVDFSTSETVHLYRSAAQQGTR
                     ILSAISNYNREDLTELKKLSHKTAVLYSPNISIGVNLLLLTAMVVSNVIPQADVAILE
                     EHFSEKPDVSGTALRIAKKLKLDPATCVKSIRAGGIVGKHEVLFGLPNQTLRICHESI
                     TRSAFGRGALTGAEWLMECPKGFYTMEQLVASRFRDALSELR"
     gene            7767..8234
                     /locus_tag="PK576_00365"
     CDS             7767..8234
                     /locus_tag="PK576_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60075.1"
                     /translation="MKEVSRRQTLAALGAGTCACGWNAGCATLSKKGNAPEIPAEAYT
                     LEGRAVTIRLNRVPSLAAVGGSVKILDARLPQPMLIGRTGENAYAAVSLLCPHRGVEV
                     EYRHADALFRCASLGHSKFHIDGKLKKGFAKTGLTRYEAALDPADPSYLRITF"
     gene            8248..9945
                     /locus_tag="PK576_00370"
     CDS             8248..9945
                     /locus_tag="PK576_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60076.1"
                     /translation="MRKQALFAVVGMMPVLASGWGAGHDTVARALALRMPEPWCTHLQ
                     GEVLRTFCADSHYPDSFLKLSEDPRITPEEKAFLEGCNPKMTVRYHFHSDTGRGVAFT
                     LMVRALRENRRESALLWFAILGHSTADMVACNHDPIAHLATYAWGDKGWGLRFPDGTP
                     MAAMQSGLDLGWVEKESAAKAVWDARVANMSATDSGKSADEALLDVMRAGLDGVGVCS
                     AHGLPIVEAAAEWTMTRKAESCQELAKRLSDLGCWAVERLLRDFLTAQRLAAGDSDPS
                     MTEAVRQRFNVWFDEFVANRNYNEDSFVKGLTTAVRPDAPLLGVLSEPTWRMNEGMLG
                     PNDRVLAAQAATSLRKRGRNAVLLDVRRVMAEGISAERVPLLLVFAQKTSPYHGMDPK
                     VLVRRLIAYRAAGGKLIWIGGSVPNPALCGFPQELVAQADVGKGYAYALTRLPVGTNA
                     YAHLSLKIGDRAAYPLSRSPNFSAGWHVPSNKTFFKSGADEHVLPIAHLLDRDRVLLV
                     GAAWPRNQPAVAYLPVYVLFPYLWTDESPVFTPFEIGLDSVGNEALEAAFAALQVAP"
     gene            10047..10568
                     /gene="ispF"
                     /locus_tag="PK576_00375"
     CDS             10047..10568
                     /gene="ispF"
                     /locus_tag="PK576_00375"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOE60077.1"
                     /translation="MLRTGIGYDSHRLTDHRRLVLGGVEIPHSRGLIGHSDADVLIHA
                     LCDALLGAAAEGDIGQMFPDTDPRWENADSAVFLTAVIERLDHKGWTVVNTDATVITE
                     APKLQPHLPAMRARLAALMGIEADRVSVKAKTNERMGAVGRHEGISVLALATISRRAT
                     TGSSRRNERGDES"
     gene            10565..12010
                     /gene="cysS"
                     /locus_tag="PK576_00380"
     CDS             10565..12010
                     /gene="cysS"
                     /locus_tag="PK576_00380"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013182431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE60078.1"
                     /translation="MMQVYNSLSRSKEPFTPLNPETVGLYTCGPTVYNFAHIGNFRAY
                     TFEDLLRRTLIFNGYRVMQVMNLTDVDDKTIRGARQAGVPLKTFTAPFIQAFFEDLAT
                     LNIQRAEVYPAATDHLPEMLDLIGKLMERGLAYRSDDGSVYFSVSAWPAYGKLARLDR
                     SALRAGARVSHDEYEKESYGDFALWKGWDEQDGEVAWDSPWGRGRPGWHIECSAMAMR
                     YLGETFDLHTGGIDNLFPHHENEIAQAEGATGKPFVAYWMHCAHLRVNGEKMSKSLGN
                     FFTLRDLLSRGWAGREIRYVLLNGHYRQPLNFTFEALDAARVSLARIDECVDALTAAA
                     GGSAPADALPDWAASARVAFTEAVNDDLNLPEALAALFALVRESNTAMRDAALTPQGA
                     AAVLDLFDRLDTVLGVIRFGRADRDSEMPPEVAERVRARAEARAAKDWSAADRLRDEL
                     ASLGWEVRDSKEGQKVKRNETMTRSTAVTAS"
     gene            12007..12696
                     /gene="tmk"
                     /locus_tag="PK576_00385"
     CDS             12007..12696
                     /gene="tmk"
                     /locus_tag="PK576_00385"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE60079.1"
                     /translation="MTQNGNTRLQGKFITFEGPEGGGKSTHIRLLETFLRARGLDVTV
                     TREPGGTPTGETIRALLQHDQAGESPVPRTEVLLFLASRAQHVDRLIRPALARGEWVL
                     CDRFEVSTFAYQGYARGFDLDMLRRLNTFATGGLTPDLTLLLDVPPGVGGARLRDRHA
                     RNAAAGLDRFEREAKPFHEQVRRGFLDLARLAPDRIAVVNTDEETDRVAERIRETVTR
                     CLLAGESPDGC"
     gene            12686..13657
                     /locus_tag="PK576_00390"
     CDS             12686..13657
                     /locus_tag="PK576_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOE60080.1"
                     /translation="MDADAKFAMVHRAIEIGHPAHGYLIVGPVRGAALTFAERILQTL
                     FCRETVRPCGACEGCRRVTTRLEPDIHWIFPEKKSRIISVEQIRENLIREVTQTALSG
                     GWKAGVLVGADRMNDASANTFLKTLEEPPEKTIFLLLCENPQSLLPTVVSRCQRIDLD
                     LFRDLEEPWKSRVLEILASPLWGQTLENLALSSALAAILGEIKEEAERRVREETKQSA
                     AMVEEDDDVLEARVSSRYREMRADFQLTLLRWYRDLMVLRAGGDPGVVFHKPWLEVLR
                     ERAARLPLDRTFANLGFIEDSVRQMERSIPDDTVLAYTLDRIDHGVV"
     gene            13668..14576
                     /gene="ricT"
                     /locus_tag="PK576_00395"
     CDS             13668..14576
                     /gene="ricT"
                     /locus_tag="PK576_00395"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOE60081.1"
                     /translation="MLTLARIRLPENSVYQCRVPASVTVVPGEVCLAELEYGLDYGTV
                     LDLTGHTELGEGEGRGPAFRIVRKQGAEDVSRLNENAQLAEKATQAFQLSVSREKGHV
                     KLLHTRFSYGRDRFFIRYTAQIPVDLRRFVGQIQRDFKTHVDLWQVGIRDETALLGCL
                     GSCGRAACCCTWQKQFEPVNVLMAKSQEMSLNPVTLNGTCGRLKCCLRFEYEQYCEYG
                     ECLPPLRSVVTFPAADETRTDVEEEGQVIGRDILRGRLTVRTRDGRHMTVSAKAVTAK
                     RNREREYGTGKEDDNDANSRGEWTES"
     gene            14542..15000
                     /gene="aroQ"
                     /locus_tag="PK576_00400"
     CDS             14542..15000
                     /gene="aroQ"
                     /locus_tag="PK576_00400"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOE60082.1"
                     /translation="MRILVVNGPNLSRLGMREPAWYGRETLEQIMDRVRALAAERHVE
                     VEDMQSDSEGELVAVIGRARERFDGVVINPAAYTHTSVALRDAIAACGLPVVEVHLTN
                     THRREPFRHASLTVPVCLGQVMGFGGTGYLLALEGLLRHLQTEKEKESNT"
     gene            15000..15923
                     /locus_tag="PK576_00405"
     CDS             15000..15923
                     /locus_tag="PK576_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE60083.1"
                     /translation="MARKQDAVRLCVVGSIGLDDIRTRTADRKNLLGGSCPYSTVAAS
                     FYTRAGAVGVVGSDFPKSFDRRWSAFGVDLTGVQRQNGTTFRWACEYDADMIERTTLK
                     TELGVFAAFQPVLPAAYQRAPFLLLGNIQPELQEQVLDQAKGARFVALDTMNLWIEIA
                     RPALIRVIRRANLLTVNDGEARQLMGKWNLIECAAALLKMGPSYIVIKKGEHGAMLFS
                     KRGIFLVPAYPVRRLADPTGAGDSYAGAFMGYLARAGRVSERLLREALFHASVMASFG
                     VEGFSFTRFERLDHKTIDARVRELRNMTATS"
     gene            complement(16047..17111)
                     /locus_tag="PK576_00410"
     CDS             complement(16047..17111)
                     /locus_tag="PK576_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60084.1"
                     /translation="MDRKENGHVWGVGIVGTGLIAHFHARAVAETPGARLVAVCGRNA
                     ARTAAFASTYGCEAVSSLEDLVCRSELDVLMIATPSGAHLDAAIAAARAGKHVLCEKP
                     LEISRARIDTMIHAHREAGTRLGCIFQLRYMSALEPIRAALREGRFGTLTYAGAYVPW
                     WRDAAYYHDSSWRGTWKGDGGGALMNQAIHMIDLLCDLLPPVISVSAHVSAVGHPGIE
                     TEDSAVASLGFEGGAVGVVYGTTAAWPGQAKRLEISGTDGTAVLTDDRLTRFSFREAR
                     DSDSSWVSHAPCETAAGAAQPGAMSHALHAACLGAFLESLQNGTPFAICGESARRPVA
                     LIEAIYEAARTGQTVRPNPD"
     gene            complement(17225..18571)
                     /locus_tag="PK576_00415"
     CDS             complement(17225..18571)
                     /locus_tag="PK576_00415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE60085.1"
                     /translation="MNVRKAWWWSVLGRAGGLIVLGVGLVSPTGCVTNPITGKQQISL
                     VGKSELISLSQQAVPSQFSSDYGVVSDAQLNAYVAQVGRRLVATLKPSDVVFPDMPFS
                     FQVVNAVYVNAYAFPDGTIAITRGMLAELENEAQLAAVLGHEIAHVNCGHTASAMSKG
                     TVYDALVSGTQGYLASRGSAWTDLAGTAGQLGSAVVLASYSREQERQADQGGMLYLVR
                     AGYDPQGMVDLMRLLIRISGSNPSMIEQMFSSHPMSTERLETAETRLREEYAGQNVGV
                     VNQAAFLAATASIRKNRAAFQHFAAAEALLAQKQAAAAKTRAEQGLRIAPNDYVGLML
                     VAQAERQSGQIGAAQQTALRAVRLYPAGARAHGMLAQCALQKKEYAAAVAHLQVFERQ
                     VPGEPHTSFYKGLAYEGMGRKPQAAQAYRAFVRRSGTQSRAAQYATQRLQHLTGMP"
     gene            complement(18568..19590)
                     /gene="fmt"
                     /locus_tag="PK576_00420"
     CDS             complement(18568..19590)
                     /gene="fmt"
                     /locus_tag="PK576_00420"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE60086.1"
                     /translation="MRIVFMGSSDASATALRGILRLPLLNVVGVVTQPDRPAGRRQRF
                     TPCPCKAYATRKGVGPIISPEKVNAPEVLDQIAQLRPDVIAVVAFGQFLGKRLLALPP
                     YGCVNGHFSLLPQYRGAAPVQAAIAAGDPVSGVTIMQMAEGMDDGDMLLKAIEPIHSD
                     DTAATLMDRLAILCAVTLAKALRLMQEGGLKREPQDHAAATYAPKIQKADGLIDWHAS
                     AVVIERRIRAYDPWPGAFTFLPERLNGKQGNKARLKVFQAEVWRDPVPHMQDYVPGQI
                     CEISPSGPIVVTGNMPLRLTGVQLDGSRRMDGKSFLNGHPMQPGDRFSAVAAMQPDPE
                     MSGGVQ"
     gene            complement(19723..20310)
                     /locus_tag="PK576_00425"
     CDS             complement(19723..20310)
                     /locus_tag="PK576_00425"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOE60087.1"
                     /translation="MTIMTETILQTPLSEAAVRGLNAGDGVRVCGRVYTGRDRLHKFL
                     MEGGACPVSLRDGVLFHCGPVVVPENGGWRVVAAGPTTSIREEPYMATLIERFGVRAI
                     IGKGGMGDKTREACVRFGCVYLQAVGGAATLLAQRVSRVDGVSFLEEFGATEAMWHFE
                     VNGIETVVGMDARGRSLFDEVREASGGVLKKLLGM"
     gene            complement(20307..21179)
                     /locus_tag="PK576_00430"
     CDS             complement(20307..21179)
                     /locus_tag="PK576_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOE60088.1"
                     /translation="MDWTETLAELIRRTTSDLPRDVEAALRTAEAAEPAHSQAARLLR
                     AIGENTRLARAQSTPLCQDTGTLTFFWRVPRESDTLALEEATREAVALATRNGWLRRN
                     TIDTLTGSSVDENVAEDCPVCHIEQADTETTEVWLLQKGGGCENMSTQFSLPDEALGA
                     GRDMEGVRRCLLQAVWQTQGFGCAPGILGVCVGGDRAEGYRVAKQQLLRPLDDEAGIP
                     ELAALERRVLTEANQLGIGPMGMGGRTTLLGVKITARTRLPASYFVTVAYLCWACRRR
                     GIRCGRGGEREWLG"
     gene            complement(21212..22084)
                     /locus_tag="PK576_00435"
     CDS             complement(21212..22084)
                     /locus_tag="PK576_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE60089.1"
                     /translation="MIDLHLHSTNSDGSDTPEELVISGCQAGLTALSLTDHDTLDGTE
                     EFLNACRANGMTGISGIEISAHLDEGPGELHILGYGLNPTHPLVVESLDRVLEGRAWR
                     NEQILEKLNGLGLELEWADVLAYADEHVVGRAHFAQALIDRSYVSSCSEAFERYLGKG
                     APAYADRYRLYPEEGIRMIREAGGVAVIAHPFSWELREAELETGLRELKAYGLTGMEV
                     LYSEYTREQTVTLMRLAKRLDLLPTGGSDYHGLAKSDIRLGTGFGSLHVPDAYLPPLL
                     NAIGSDSAWVVRPD"
     gene            complement(22081..22773)
                     /locus_tag="PK576_00440"
     CDS             complement(22081..22773)
                     /locus_tag="PK576_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004073255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60090.1"
                     /translation="MALNRVRDVDNRVAVRRVLMSVSDKSGLDVLVPGLLETCPNVVI
                     YSTGGTFAAVRDMLDPAVAARHLVAVSEYTGQPEMQGGLVKTLDFKIYLGLLSETYNE
                     AHRQDLSRVGAEPIDMVVVNLYPFSQTVARAGVTPEDARTNIDIGGPCMVRAAAKNYL
                     RVASVTDPADYAALLEELRRTGGTLGFGTRLRLMKKAFAHTAGYDQAIAAFFADVAEP
                     DARSAYEVRDRL"
     gene            22924..25095
                     /locus_tag="PK576_00445"
     CDS             22924..25095
                     /locus_tag="PK576_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60091.1"
                     /translation="MMRTVSRMMLGMLAMITWAEAGTSKTFHVSPNGCDTASGSWWRP
                     FATFERAQQAVAEFKARHPAEAIERIEVRFRGGTYPLKQPIRIRPEHGGTATIPVIYR
                     SSGDAPPVFTGGRLITGWTVRADGAWTTHLPEVKSGSWDFAQLFVNGARRFRPRIPRK
                     GYFTTTNDFETVEAGVNGFYYKEDGLDPAWSNLQDIEFHTLHIWSASRVRLKSIDASR
                     KIVRFLKTRNYNQYWGSYKGPGWGENGCRFWAENVKEAFGVPGEWYLDRPTGTLSYMP
                     MPGERPDTARVEVPILPLLLIVEGDADRPVINTRIEGLVFKQTCWTTPESGNFIPQAE
                     VNIPAAAEFVNARNVTVSRCVFTQLAGYTFAFGPGSHSNTVDACIMSDLGAGGVKIGP
                     PYAGYSMFESMRNDFQSKNELADMDKTSAITISNCRIVGGGRIHPAAHGVWVGHASHN
                     RILNNEIGDLYYTSVSLGWTWGYAEPSRAHHNEVAFNHMHHIGQGVLSDMGGVYTLGV
                     SPGTTVHNNRIHDINAFTYGGWGLYTDQGSSYIHMYNNLVYNTKTGGFDQHFGRENIV
                     ENNIFANARKDQVQRSRDEEHISFFFRNNILYWETDGNLLGNNWKSGTRGMKDGKPTQ
                     HYELGPNLYWHTSGKQELFPGKKTLAQWQAETGQDAGSLVADPLFENPAAGDFRLKPG
                     SPATQVGFKPFDTNAAGPDKSARLPKFKLRPVPTMYEITTP"
     gene            25106..26293
                     /gene="eboE"
                     /locus_tag="PK576_00450"
     CDS             25106..26293
                     /gene="eboE"
                     /locus_tag="PK576_00450"
                     /inference="COORDINATES: protein motif:HMM:NF035939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metabolite traffic protein EboE"
                     /protein_id="HOE60092.1"
                     /translation="MSISYCLNIHPGESLDAVRQAITRHACAVKARVSPAAPYPLGLR
                     LSAAAAGELSDPAALAAFTDLLGCNDLFVNGINGFPYGTFHNTAVKTAVYQPDWSTPE
                     RLTYTGRLATLLAALLPENQTGNISTVPIGHKTTAQPATSSLPVRQITIMAEFLHDLK
                     QRTGREVVLALEPEPDCLLENTQDVIVWFEDGLLTEGRRWLCASRRRTPEEAESLLRT
                     HVGLCLDTCHFAVEFEDPLAALARFESAGLRVARIQLSAAIRAVISDASLARLADFID
                     PVYLHQTKIQLPRGPLLSLPDLTNETLMTARRHMGRELRTHFHVPLFYAGDGVLDSTH
                     TDLTPEFFAHTVAQGYPLEIETYTFNVLPSALRPASVIDSLVAEHDWVSVRLHRHHQQ
                     ERL"
     gene            complement(26422..27012)
                     /locus_tag="PK576_00455"
     CDS             complement(26422..27012)
                     /locus_tag="PK576_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOE60093.1"
                     /translation="MRKCFAHTARHQEDWEPLKTHLLNVAARARQFASIFGAGVEGYI
                     AGLLHDLGKYGIRFQERLHGKGRHIDHWTAGAWTAMKQYGKAGYCLSLIAQGHHVGLQ
                     QDTKESFKKICSRAPSPDTTLSADSMNELLQLFAEDGLSWPTLQKSFYSRTGKRCSDM
                     LDVRMLFSALVDADYLETEKFINTVEVKPSRPTRGA"
     gene            complement(27153..28304)
                     /locus_tag="PK576_00460"
     CDS             complement(27153..28304)
                     /locus_tag="PK576_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOE60094.1"
                     /translation="MRTLKTIAAGHVCLDIIPTFLRGAPTLEAVMQPGKLVDMGPAVL
                     STGGSVANTGMALKRLGLPVRLMGKVGRDAFGAAVCDVFRTHDETLVKTMIVDPAVAT
                     SYTVVINPPGIDRIFLHCPGANDTFGADDVIPQLLDGMDIFHFGYPPLMRAFYTKTDE
                     LMRLFRKVKECGLTTSLDMALPDPQSPAGQADWRAILKQALPFTDIFSPSLDEIVFML
                     HRERGLKVREQAAEGVPMGGLIEEDVRALADELIALGVAVVMLKLGNQGAYLRVTSDE
                     TRLATCGCFDTASRTAWGGVEFFAPCFDAKVAGTTGSGDCTVAGLLAAIAKGLTPAES
                     LRTAVAVGSASVEQPDATSGVPTWTALDARIRAGWGRETPKVAFQAKWV"
     gene            complement(28339..29268)
                     /locus_tag="PK576_00465"
     CDS             complement(28339..29268)
                     /locus_tag="PK576_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOE60095.1"
                     /translation="MARAISKLAPGWWDYTTLDSEILNDAAALTAKDLEQLSRPGFKV
                     CIYDTVEEFYAAEALEYIEAWRQATPDNPTGVCGPIGPTEHLPLVARIVNAIGLNLRD
                     AHFWGMDEWVENGKIVSTDHPLSFSKCDREMCFDRIKPELSMPAGNMHFPTGDLAAFT
                     RSFDEIKCLLMQGGQGEIKHWAFNDPVKREGAYADAPPSPEEYRKLATRLVDLHPVTV
                     IQNARTSGGGNVALVPTSASTVGPVETWKSAKVSIWHPGHHDNPFGIRLSALMISKRI
                     PDSSVPMSLLADHPNVQFNYLRSGIGEVAVEMH"
     gene            complement(29320..30105)
                     /locus_tag="PK576_00470"
     CDS             complement(29320..30105)
                     /locus_tag="PK576_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE60096.1"
                     /translation="MSEKMVALAMAAHPDDIEFMMSGTLLLLKQAGAEIHICNLANGC
                     YGSQVYGKEEAARVRALEAQEAARVAGAVWHPSLFDDVGLFYNADSLAKASAVFRKVC
                     PDILLTLSLYDYMEDHEYASRLAASAAFNRCLPSYVTDPPLPSTNKPVAVYHSLPHSL
                     MDMLCRPVEPEFCIDIGSVMEVKREMLACHTSQREWLDATQGMGSYVESMADAAREVG
                     RRYGGCEFAEGWRRHSHMGYCAPDFAPLETALAPLLRRVAPST"
     gene            complement(30145..31203)
                     /locus_tag="PK576_00475"
     CDS             complement(30145..31203)
                     /locus_tag="PK576_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60097.1"
                     /translation="MKTFGAVRALDGVSVSIRPGELFFLLGPSGCGKTTLLRCLAGFH
                     TPDEGRIFFGTQDVTDLPPHARNTGMMFQSYALWPHMTLAENVAFGLEMRKRPRAEIR
                     ARVAAALELVHLADRAKARPNLLSGGQQQRVALARALVVEPQCLLLDEPLSNLDAKLR
                     LEMREEIRRICKAAGTTAVYVTHDQKEALSIADRIAVMRDGRIEQIGTPEEIYRHPAT
                     RFVAGFIGEGHFLTGRIESVSETEGVQVATRIGLFQARRAPGGIRTGEEVTVCLRPET
                     LRFGVPPHAGMNRMRGRYRASVYLGEMARHEIDLGPEDGPCEVLTAFQLNPSGGTDRT
                     GDPAEVWVAPDEVIVTRG"
     gene            31436..31720
                     /locus_tag="PK576_00480"
     CDS             31436..31720
                     /locus_tag="PK576_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE60098.1"
                     /translation="MILKVALRAIFRNKTRSLLTALGIIVGIAALIAVVASGQGAQAM
                     MVREISSIGNNIIMVFPGSRGALHGWRSDRCIGWHLRFLSRPPRLPSQSH"
     gene            31744..32433
                     /locus_tag="PK576_00485"
     CDS             31744..32433
                     /locus_tag="PK576_00485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE60099.1"
                     /translation="MTMTLSSSLLDFGDVRAALGRGGRVALFIRHSERPPIRSDDKDF
                     GRHLGLTPRGVAVAREAGAMLAGAPDVRLLASPMQRCRLTARHIAEGMGVPEPQVEDA
                     DPLGVRGFFYRDPYAVQEIMRQQGYMAYMLEYLKKGNALHSAPLMTATEQTLEWMITQ
                     MTVPFVLFVSHDIFVAAILTALRLRSYTAEEWVGFLHGFALIQHGQDPWACHPCLPSH
                     TEVDASHPFIH"
     gene            32448..33074
                     /gene="thiE"
                     /locus_tag="PK576_00490"
     CDS             32448..33074
                     /gene="thiE"
                     /locus_tag="PK576_00490"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOE60100.1"
                     /translation="MLIERFGLYLVITDPVTSYERCAEAAVAAELRYIQLRRKKCDRA
                     AILATAHNLRTITRGTSTRLIVNDDPELAAEAEADGVHLGQDDMPLPEARRRYPVLRH
                     FGLSTHTPEQARSAIKVAPDYCGVGPVFATPTKEVPDATLGPEQAGAIIRAAPFTTVA
                     IGGIDADRLPEVLRAGAVNFAVVRYVCQAPAPFDAIRRLQDIWQAAQP"
     gene            33112..35556
                     /locus_tag="PK576_00495"
     CDS             33112..35556
                     /locus_tag="PK576_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOE60101.1"
                     /translation="MMRCLLIPLLFYILCRTTASALPEINLITEPACNSEVAVIVGHG
                     FVPGRTRVKALALGGYDMRKPQDPLRFAKAIGRPPDLPDTPPPEAQECEIVGQGEVFL
                     QVLFRCLPQSWVCAPLTSAVWVGDESGWGRPILINRPQAQWLYPQRQAPGEPIRIFGR
                     TFAWGWLLPSAHVLLRPVGSTQMVHLARAAQHHEDGHTERWSLCVWLPESLAEGEYEV
                     FVHGRHGAEYGWSSPLRLTVAQKPSSRPVVSVREHGAVGDGLADDTAALEAALRQAAP
                     GGTLFLPAGTYAISRTLNIPEDVVIQGAGMRQSVIANRQEPCVDPFALTQTRPVFDGK
                     DLLWGMRRFALRDLTLRFMPALGSALRVGRDPLWSEDVSLYRVRLETQQDFGLSPTHP
                     YCARPLNIVKARRFSMVRCETYGPGACSCERKVEDSLFSQNRFITDRRWRGHGFKFWG
                     AEQVIFEDNLLSGDTRGLVMQTHFGVNYRNFIAGNTVERTVLGENAGETYLVEGAGYL
                     GESAVEEATPTHVRIARWPTARGAPATAEMCVGRFVVIAGGRGIGQWRRVRAADPAQR
                     VLYPETPWRVPPDASSVVVMMNGLIDTVFVNNQETDCGKGLYIYGAGAINCIIDRHLC
                     ARTLGITFKTHDDRQNADPMHHQTAPDFFNLIRDCRVHDGGGLFCAVGGRLPLEERNG
                     VPWANFANRFIANEIQNVTPFSGAQYGANWTWGGGWDNVQAGISVIPSDLGTAAGSGS
                     PGPERIRAATFQKNWISGSRFGVGVSRRAADTLLMQTTVYDVDVPLTDKGRDTREKAT
                     VVRTPETYSPERGPIR"
     gene            35641..37221
                     /locus_tag="PK576_00500"
     CDS             35641..37221
                     /locus_tag="PK576_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60102.1"
                     /translation="MRNQWQTKQLARRCVIALPFLLFLVSSGCKPSAKRAAEREAYLK
                     HEVEQTSLRIQDYSYERQDEALVLVDEALANPRFAGHKPIFFAHKINLLLSLERVKEA
                     SDLVVEAWKKAPDQAQAVFGKICDFHQQRNAYREMNDWCTRLLDLGAAFPKELRGRLM
                     LRRLTAALGIKDMEQARSDLDTLLTLVRPEESLPDLQRSLEWFIDMGNHREALVLLNH
                     FEKAAAGQPSYQPLIVSLKLRCIVLTGEWGRVVEALAACVPQLPDAMLHQTLKNVFGA
                     LNKNNQTDLLQQAAKQILFNAVGKEASVHYAARVWVDTGMTVDKKVLAERLNVLLNVK
                     VSPILVGDLYERYFYETIEDHNVVRNLCAVGERLLTVCTNEATVNMAKVKVLDGAFIV
                     NDYDKAIHMLEQGIPGKDKAWHDMSLPKVKAHRAMAQNKPREAVAYFRAFMNAWLATD
                     QDEESDPTTGISHSREWILGRNAIRIARLLESIPDKEEADKAREEAKDYFRKALIKAE
                     NDPPALKLLKEEIRPLGL"
     gene            complement(37253..38335)
                     /gene="ispG"
                     /locus_tag="PK576_00505"
                     /gene_synonym="gcpE"
     CDS             complement(37253..38335)
                     /gene="ispG"
                     /locus_tag="PK576_00505"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOE60103.1"
                     /translation="MRCPRTATRQVHVGSVAIGGGAPVSVQTMANADPHDAEALLRQV
                     CEAAELGCDLVRLTVPDHAAAETFKQVRARSPLPLVADIHFDHRLALAALAAGADGLR
                     INPGNIGGPERVRAVVAAARPNRIPIRIGVNGGSLEKDLLARYGSATPEALVESALRH
                     VAWLEAEDYREMKISVKASDVPRTLAAYRLLSERTDYPLHLGVTEAGTFLPGTVRSCV
                     ALGTLLLEGIGDTIRVSLTDQPAQEVRVGVELLRSVGLRAPSASVTSCPTCGRTRVDV
                     AGLALRIENRLERFYREHPDAPRPHVAVMGCVVNGPGEAKGADIAVAGGDGVFVLFVR
                     GERIRTLPEAEALEAIVRLVTEWPRP"
     gene            complement(38361..39806)
                     /gene="rseP"
                     /locus_tag="PK576_00510"
     CDS             complement(38361..39806)
                     /gene="rseP"
                     /locus_tag="PK576_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOE60104.1"
                     /translation="MEWVSLIGSGVCVALFFSLAIFIHEFGHFIAARRLGLRVDAFSI
                     GFGPALWKRTVGGVVYKVGCVPFGGYVALPQLDPSGMEKVQGSERDGEGDSCSELEDI
                     APWKRIVVALAGPVGNFVLAIFLAYFLFLAPGVKTGVLDTRIGSVAENSEAWKAGVRP
                     GDRILSVNNRKVGTWSDLQVECQLAGDSGKAVFGIQRGDTVREVELPFRTSEIVELKM
                     LEGVFPATHCEVSALMPDMPAIQAGLLPNDVVVEINGEPVREAQQFAEAIQHNRAQPV
                     TLTVKRGRELQQIVLTPRFDEASGRYLVGIVWKDATERVRAWMMYRNPWRQLKWDSLS
                     VVRVLQALLAPESKGERKAVARNVGGPVAIVIGLFHTVRGDILDAIGFLRMICVNLAI
                     LNLLPFPVLDGGHILFALYEVTTRRKPHPKVVSALVNAFAFLLIGLMVLLFYRDIARQ
                     VKISRAVRAAVREARQEAETASETNASVVPR"
     gene            complement(39872..41080)
                     /locus_tag="PK576_00515"
     CDS             complement(39872..41080)
                     /locus_tag="PK576_00515"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOE60105.1"
                     /translation="MRSVVILGSTGSIGENALRVIEAHPEALRVAGLSTRTHIMRVLD
                     QALRFDVPVVAVADENAAREAETLARPHGIEVWPGEAGVAALAALPKADMVLCALVGL
                     AGLEPVLEAIRVGHDIALATKEVLVAAGELVMRRRAAAGVRMLPVDSEHSALFQCLQT
                     PGCPPACVRDAADTSRIEARVARFALTASGGPFGASKETDFDRVTPEEALAHPRWDMG
                     RKVTIDSATMMNKGLEILEAHWLFNLPVAKIDVLIHPESVVHSMVTFTDGATLAQLSM
                     PDMRFPIQYALTWPERLTADLPRLDLARMGCLTFAEPDTTRFPCLRLAREAAQAGGTA
                     PAVLNAADEVAVEAFLAGAIPFSGIARAVEAVMAQHDVKTCVELETILEADAWARRQA
                     GAQVKKSGTT"
     gene            complement(41077..41985)
                     /locus_tag="PK576_00520"
     CDS             complement(41077..41985)
                     /locus_tag="PK576_00520"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE60106.1"
                     /translation="MLMRRILAGVLVGVAWLLSLCAMPGWMLFGVLLVMSSACQNEFY
                     QMMKRNGYPVSRNGGMVMGVVWLTACYAFPPYAAGRLPTGHQFETLMLAMLVAALLLR
                     VLFDSRIAKPVEHVGVTLLGFFYLPFMLSFFIRLAQWGADEMFEIPSARAGVFLASYL
                     AAVVKFSDVGAFAVGVTMGRHKLFPRVSPKKTWEGLVGGIVVSMMVSAGLVAAAKRFD
                     CLPGGPLAELSMTHALVLGLVLSVVGALGDLIESMFKRSVNAKDSAGLLPGGGGGILD
                     MFDSLTFTPAVLYFYLVWFVGQSVAL"
     gene            complement(41979..42728)
                     /gene="uppS"
                     /locus_tag="PK576_00525"
     CDS             complement(41979..42728)
                     /gene="uppS"
                     /locus_tag="PK576_00525"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOE60107.1"
                     /translation="MDTQTNRVPSHIAIIMDGNGRWARQRGKPRLAGHEAGSETVRCV
                     LNYCRDAGIRYLTLYAFSTENWSRPPDEVSGLMALLGTFIHKHERELIARQVRLRVIG
                     QREDLSAPLREALTRLERETAHFERQLLIALSYSGRNELARAARRIAEEVRAGTLTPE
                     QVDEAAVAARLDAPDVPDPDLIIRTSGEWRISNFLLWQSAYSEFFVTPVLWPDFREAE
                     FQAALAAYAKRERRFGGVTAQPAGAGGGAPC"
     gene            complement(42763..44043)
                     /locus_tag="PK576_00530"
     CDS             complement(42763..44043)
                     /locus_tag="PK576_00530"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOE60108.1"
                     /translation="MPNTVLVGAQWGDEGKGKVIDVLTSQADLIVRYQGGSNAGHTVI
                     ADGRKYVLHLIPSGILHTDKQCVIGNGVVLDPFDLIREIEGLREQGFDPLGRLYISDR
                     AHMVMNWHRALDAADEARRSQERKIGTTGRGIGPAYGAKIGRSGLRAGDMLSPSFLEM
                     VEAGVAEANTVLAGWGAPLLDGRETRVRYEEAVRTLTPYIADTVTLVNEADRAGKSIL
                     LEGAQGTMLDIDFGTYPFVTSSSPTAGGACTGSGISPRRIDRVVGVIKCYTTRVGAGP
                     FPTELTGETGERLRQKGGEFGATTGRLRRCGWFDAVVGRYAAMVNGVDFWAMTKLDVL
                     DEEPVIRICTAYERDDGVRYEIFPSDLSVLERCTPVYEEMPGWLTPTSCCTRYEDLPL
                     KARAYVERLIALIGGRLGILSVGPAREATLRLGL"
     gene            complement(44196..47252)
                     /locus_tag="PK576_00535"
     CDS             complement(44196..47252)
                     /locus_tag="PK576_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60109.1"
                     /translation="MTLLGTLVFAVVHLGAVETVIDAAETPQIEKETVLAVGDVLKLV
                     GKKADFVATAPIRGAGATLYGALGSPGSCTHQAVFEAPIEFDTVLWGQDYGESKLVLK
                     ASGNRWHKFELAYAKVECAAANVLAPEGVLTWGDYYRGTLDRNATFDLGGFDQTIDRL
                     HSTSSPGTKNSDHVVSTGGTLLMRGTADAVCFARIDGELNLVWHPTGDFTQSFDERVN
                     SVRGRIEVRRGTVRLGKSGAFRNIDEIKVADGATFVVEATNALALAAVKLVELGKDSA
                     LVVKGVDPFMPQQVSVVKDVGAKIESAVPLKLAREAAYDDTPPRTIRDKLQVFWDDWI
                     VDPAMTSARRVPHHPEYVGPVITTDKPWEGASGSYMSVVKVDDVYRMYRQGMSSHESE
                     PHYHGLKICLFESKDRGLTWERAKINRFPYKGNRENNIVIDFRVNYWDNFMAFRDDNP
                     ACPEAERYKAIALYSHKWSEEFAKAHPDYAPLAKYGKALWCFVSADGLDWKPGWPLFK
                     FGDMGLRFDSLNVAFYDEVNREYRMYMRNVKPLEYERYGGTNARWVMVTRSKDFHNWS
                     KPENIDFNDGQEEYELYTSGVDLYPRNPELTVGFPTRYTDRRRWSKNFERLCGRNKRV
                     ERLAPDPRHGIAITDTVFMWSRDGKTFTREEDAFFSPGPEHAGNWMYGDGYLQRGFVV
                     SPGRYGSDDELSFYEVDGGWTGSRLTINRYALRMDGFVSYHGRYRTDKLVTKRVVFDG
                     NRMLLNFKTSARGRVFVKITDEAGKSIVTDEIFGDKVDRIVDFEDGEVSAFAGRPVRI
                     EFGLSDADVYSFRFEKAADAGARDACPYRGRSGEYKTEIEKTCWSNMTDEVRIPYLAA
                     LSMEYRVTAERETMLKADELFMVLKDTQRLNATVTEGQPLFGELLAVVDCHWRIARDP
                     WQIRWFYSRLGACDHPLALAFNVKYSRNDQAKAKLKELLAAGRVRPEELPVTWDRLNN
                     EDGYVQERFADRLVKDFDLTSQPQDAAGKARWLWAYWTMRLLNNGR"
     gene            complement(47295..>47706)
                     /locus_tag="PK576_00540"
     CDS             complement(47295..>47706)
                     /locus_tag="PK576_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60110.1"
                     /translation="GGAAKIVVGAEGTGMIEIGSNGVVQAEHVVMSNGVQSVLRFVAG
                     ANGFGYLDAQKSLSICDGQRVEVDLTEYAGSKTKHQLVKFSGTNENFRVDVLQLDAVF
                     TDESGKNQHKVAVLRKGADGLYLNLMQGTVLHFK"
BASE COUNT         9462 a        15034 c        14126 g         9084 t
ORIGIN      
        1 accgcctcac agtctcacag cctttgagat tgcgggctcc gcccgcatct caatacggtg
       61 cggcggggac cgcgacaaaa cccaatgttt acggggcttt gccagaattc tacccgttca
      121 acagcttctt ttgcgtttag ggagcgtccg gcacaagcaa tgtggcctcg atgatcgacg
      181 ccgcatcggc caccagacgt gcgcatgggc gcgtggcgta ataggcggtg gtgcgttcgg
      241 aagggactgc cgaggtttcg gagcgtatcg tccccagcag ttcgcggcag acaatggaac
      301 cgtggcgcgc ctgaaacgcg gacgccatct gccgcaccac ttcgtaagtc ttgcgtttgg
      361 cctccgcatc gccgggtgtt tcgcttccgt atttcagtcc ggccagaagt gccatagcag
      421 aaaccgcccc gcagacatta cgcaggcgtc ccacaccggc ccccaatccg caagacaggc
      481 gcatggcgca tgcttcgtcg ataccgaaca ggtcgctgta agcggcgaaa acagcctgcg
      541 agcaggcgca tccggcatga aacagttcga gcgaccggtc aattcgtgca gaggttgatg
      601 acatgttcac actccaattc gcaaacgggt ttaccgctat ggaccgcacc tcgtccgcat
      661 aatctggaca gcgcattgga aacatataaa ctatagtgaa acaaagaggg gttgaccatc
      721 ttgaaaagaa gccgatgcgg cggggtattg tgcattgaca tcttttgccc gaatacggta
      781 agattactta actttttgag ataaaaaggg tgggggggct taccccgctc ttttttgttg
      841 gttgaacctt gaaagcggaa ggcggtgcgg cgatgaacaa tgagttgctt tcgattatca
      901 attatctgga gcgagatcgc ggggtcaacc gggaaattat tatccaagcc atcgaatccg
      961 cgatccagca ggccgcccgc aaaacatcgg actcaacgta tgaattacgt gtggaggtcg
     1021 accggaaaac cttggccatc aaagcctatg acacgctcac tgtttcgaat gacgagaccg
     1081 ggcctgggtt catctcgctg cggcgggcgc agaggctccg tccgggagcc aaggttggcg
     1141 aaaccgtgga agtcgccgtg ccgccggccc agttgggacg gatcgccgcg caaaccgcgc
     1201 gccagatgat cctgcagaag atccgcgaag ccgaacggaa gaacatgtac gacgaataca
     1261 aagaccggat cggcgacatt gtcaacggga tggtccggca gatttcccat cgcgacttga
     1321 ttgttgaact gggcaagacg gaggcgattc tgccggccaa ggagcgtctt cccacggaag
     1381 actacaacat cggtgaccgg atccgcgcgt acgtgtaccg tgtgcagacc ggggtcaacg
     1441 ggccgtcggt agtgctgtcg cgtgcctgcc cggatttcgt gaaaaccctt ttccgactcg
     1501 aagtcgcaga gatcgccgac gggattgtcg aggtgatggg cgtggcacgc gaccccggat
     1561 accgctctaa gatcgccgtg aaaacgctgg acgaaaaggt ggatcccgtt ggcgcatgtg
     1621 tcggcttgcg gggtgtgcgc gtacggaaca tcgtgcggga actgaacggg gaaaagatcg
     1681 atatcgtccg atggagcgat gacatcaagc agtatgtcgc gcaggcgttg gcgccggccc
     1741 gtctggagag tgtctcaatc gacgccaacg agttgcgcac tgtgaatgtg atggtggcgc
     1801 cggaccagct ttcactggcg atcggcaagc gcggacaaaa cgtgcggctg acttctaagc
     1861 tcaccgggtg gcgggtcaat atcataaaga gcggggaaga ggtcacgttt gaagagcagc
     1921 gtgaaaccgc gatcgcgtcg ctggcagagg tgctggacat cacgacggaa atcgcgacca
     1981 cgctggtggc gaacggattc cttaacaccg acgggatcat cgaggccgag attccctatc
     2041 tgcaggaagt gactgggctt gatgaggaaa cggtgcaaaa aatctgggcg gcggcccaag
     2101 cggtcagcgg cggcgaggac agggaagagt aaggggtgac gatgagagta tttgaattgg
     2161 caaagagcat gcagacttcg agtgtcgaag tgctgaagca ggcggatgcg ttaggcatcg
     2221 aggcctattc gcccctgtcc gcgctggaag accaggaggt gtcgcgcctt caggcttatt
     2281 ttgcgggtct gggtgccgag gtgaaggagc gtgcgagtgc cgaacgcgcc gaacgcgcac
     2341 gcgccaaaca ggagcgggca cggtcacgac gcatggcgca gagtcaggcg gaccgggcgg
     2401 tgttggaggc gaaccgtgcg cgcgccctgg aaatggacgt gcgggcccgg ggcgaagtgt
     2461 cgcccgaacc ggcggctccg gctccggctg cggaggccga gacaaccccg ccgtccacag
     2521 ccgctccgga gaaaccggcg ccggcaccgc agaagaagac cactcccgca accgcggcaa
     2581 ccgctcgccc gacatccgcc gtcaagccga ccgccgcaaa gccagccgcc gccaagccga
     2641 ccgctcagaa aacgaagccc gcgcgatatg cgtcagcgga agatgagttg gagcctgagg
     2701 agcagttggc cgcatccttc atgcaacgga tcgcgaagga gccggaacgc gagatgtcga
     2761 agcctccccg catgacgccc gagggaaaaa cgcgtgaaaa agaaaaagag atcggacgga
     2821 tcccgcagcg tccggcttcc gccgcatcgg ctgccgcgcg tcccggtagc cgtccgtcgc
     2881 gtttcacgcc ggtgtcacaa caacgtatgg aatccggtac cccgcccgac cgcatcattc
     2941 tgttgcgcgg cgcggtggtg gtgaaggaac tggcggaaaa actcgggatc cgggctaacc
     3001 ggctgatcgc ggatctgatg caactcaata ttctcgcgtc catcaatcaa cgcgtggagg
     3061 ttgatgtggc gcagaaggtc gctgataaat acggcttcaa agtcgagatt gaaaaagcca
     3121 agcgctcaac ggagcgcagg cccgtactgc gcagcgagga tgcggatgac accatcccgg
     3181 acgacaaacc cgaggatttg ttgccgcggc cgccggtggt caccttcctc ggccatgtcg
     3241 accatggcaa aacatcgctg atggaccgga tccgtaacac gcgcgtcacc agcggcgagg
     3301 cgggggggat cacccaacac atcggtgcct atacggtgga aatcaatggt cggaaaatca
     3361 cctttctgga tacgcccggc catgccgcct tttccgcaat gcgcgctcgc ggcgccagtc
     3421 tgacggatat cgcggtgatc atcattgcgg cggatgacgg catcatgccg cagacccgtg
     3481 aggcgatcag gcatgcccag caggcggggg tacagatcat gatcgcgatc aacaaatgcg
     3541 acctgcccac cgccaagccc gaccgggtgc gccagatgct gcaaagcgag ggcctgactc
     3601 ctgaagagtg gggcggcgat atcatcagcg tggaagtctc ggccatgaca ggcaagggcg
     3661 tggaccatct gcttgaaatg atcctgctgc aggcggacat gctcgaattg agcgccaatc
     3721 cggcgcgccg cgcggacggg ttcgtggtgg aggcccagct cgaacagggg ctcggaccaa
     3781 ccgccaccct gctgattgcg ggaggcacgc tgaatgtggg agacgcgatt ttgtgcgggg
     3841 agcatttcgg caagatccgc gggttgatcg acgaccgcgg acgccgcgtg aagtcggcgg
     3901 gccccgccac ggcggtcaag tgcatgggac tgtcgggtgt gccggaggcc ggggcggcgt
     3961 tccgcgtgat gctcaatgag aaacgtgccc gcgaactggc ggagaaactc gcccaagagc
     4021 ggaaggagat cgagctgtcc agcgccaaag ccacctcgct ggatgcgctg atgagtcaga
     4081 tgcaagagaa ccagcgccgc gagttggcgc tgatcgtgaa ggcggatacc cagggttcct
     4141 ctgaggccgt ttgcgaggcg cttcgcgaaa tcaagagcga aaaggtcacg ttgaacatca
     4201 tccacgaggc catcgggaat gtgtctgcca cggatgtgaa caaagccgcg gccggtcgcg
     4261 cgctgattgt gggcttcagt gtcgggtgcg atgccggtgt gcagcaactc gcccgtcatg
     4321 ccggagtgcg catcaatacc ttccggatca tctatgaact gcttgaattc gtgaagcagc
     4381 gcatgcttga tctgctgccg cccgaatata aggaagttat tcggggccat gccgagattc
     4441 gcgctatttt cgatatcggc aagaccggga gggtggcggg ttgccaaatg ctcgacggtg
     4501 tgattcgcgc ggatgcgcgg tgccggattt tccgcaagaa agagcaaatc tgggatggcc
     4561 gtttcgccgc cctcaaacac ttccaggacg acgtttccga agtcaccggt tcgcaggagt
     4621 gcggtatttt cttcgacggg ttcgaggcgt ttcaggaggg cgacacggtc gagtgttacg
     4681 ttctggaaga gttgccgcgc agcctatgag gtgcgctgcg gcgcaaggag gaggcgatgg
     4741 ccgttgatcg ggtagaaagg ctgaacgccc tgttgcggcg tgagatcggc gaagcgctgt
     4801 acaaagtgtt tgccagcgac ccgctggatt tggccgcgat cacggtgacg aaggtgcgcg
     4861 tcgcgcgtaa tctccgcaac gcgaccgtgc aggtctcgat tttcggccat gaaagtgaac
     4921 gcggggagtt catccggaaa ctcgccggga aagccaagga actgcaagcc ctgatcaacc
     4981 gcaatctgaa cctgaagtac acaccccgtc tccgctttga attggacggt tcgctggaaa
     5041 agggggatcg cgttctggat gtgctgagtc ggctggaggg gttagaaacg cacggcgaaa
     5101 cgaaccccgc tcccgacgtc ccgcccggct gagccgagag gaaaacccgt tgagatgaca
     5161 cgttattcga acgcgccctc ctccctgacc ccttttgacg gtcttctgct ggtggataaa
     5221 ccgtcggggt acacttcgca tgatctggta cgcctcatcc ggaaaacgtt tcgtgtggag
     5281 aaggtggggc atggaggcac gttggacccc ggcgccacgg gcttgctctt gattctgctg
     5341 ggcaagggta cgaaatgctc ggatcaggtc atggctgggg acaagaccta ttccgccgtg
     5401 atacggttgg gccggaccac ctccacgcag gacagggaag gcgaaacgct tgaggaaaaa
     5461 ccttgggata cggtgacgcg tgaagcgttc gaagcgaagt taaccgaatt cacgggggat
     5521 ttgttccaga cgccgcccat ggtttcagcg atcaaagtga acggtgtgcc gctctacaaa
     5581 ctggcgcgta aagggcagga ggttgagcgt aaaccccgct ttgtgcacgt ctaccgtttc
     5641 aacctgaccg aatggacccc gccgctggcg catgtggagg tggtctgcac caaaggcacc
     5701 tatgtgcgca cattggccca tgatgtgggc caggccttgg gctgcggcgc gtgtctcgac
     5761 gcgctgcgac gcaccgcttc cggtcccttc cgggttgagg atgcgctgcc gttggatgcg
     5821 gttctgcggc tcacgcctgc gcttctcgcc gaacgggtga ttcccctgta ccgcctcgca
     5881 gggagacgcg catgagaatc ctacgccaag ccgtttcttt ccggcggatt ccgggaccgg
     5941 tttttctggc cgtcggattt tttgacgggg tgcacaaggg acatcagctt gtgttgggag
     6001 gcacggtgga acgggcccgc gaagtcggcg ggcaggcatg ggtcctgacc ttcgaccgtc
     6061 atccgatggc ggtgctcgcg ccctcgaagt gccccccgct gctgtgcaca ctggaggagc
     6121 gtctgcaccg gctggaactg tccggagtgg acggggtcct gctgttgccg ttcacccgga
     6181 aacttgcggt ccggaagccg gaaaccttta tccgctggtt gtgcggggcg cctccgcaaa
     6241 cgggcacagg tcagaacgct ccctcgccag gcacacgact gcgtgagatc cgctgcggcg
     6301 ataactggcg tttcggccga cgcgctgcag gcacgccgga ggagctcgcc cggtacggcg
     6361 ggatctacgg gtaccgtgtg gtgatcgtac cgtatgcccg ctatcaggga gtggagattt
     6421 ccagcacgcg gattcggtat gccgtgcggg aagggcgttt gaaggacgcg gcggcaatgc
     6481 ttggacgccc cttttcgatt cgggatgtgg tggtgcgcgg acgcgggctc ggccgggacc
     6541 ggtgtgtggc aacggccaac ctgcatcctc gctcggatgt gctgccgccc aacggagtct
     6601 atgccgtgcg cgtactgacg ggcgatgccg cctataacgg tgtggcgaat ctgggaatgc
     6661 acccgacctt cacggatgtg cgccccgaac gccctgtgtt ggaaacgcac ctgcttgggt
     6721 tcgaagggga tttgtacggc caaacgatcg aggtcgtgtt tctggagcgg ttgcgcgatg
     6781 agtgcgtgtt cgattcggct gaagccctcg cgagtcagat cgcggaagat atccggaacg
     6841 ccaccctaat ttttcaacaa cacggaacgc ccggtccgca gcggtcaccg tagttccgac
     6901 agcgcgtccc ggaagcggct ggccaccaac tgctccatcg tgtaaaaccc cttcgggcac
     6961 tccatcagcc attcggcacc ggtcagcgcg ccgcgcccga aagcagagcg ggtgatgctt
     7021 tcatgacaga tgcgcaacgt ctggttgggc agcccgaata aaacctcgtg cttcccgaca
     7081 atcccccccg cgcggatgga tttgacgcaa gtggcgggat cgagcttcag ttttttcgcg
     7141 atccgcaagg cggtccccga cacgtccggt ttttcgctga agtgttcttc aagaatcgcg
     7201 acatcagcct gtgggatcac gttgctgacc accatggctg tcaacagcaa cagattcacg
     7261 ccgatggaga tgttcggcga gtagagcact gcggttttat gcgatagttt tttgagttcc
     7321 gtcaggtctt ctcgattgta gttggaaatc gcggaaagaa tacgtgtgcc ttgctgggcc
     7381 gcgcttcggt agaggtggac ggtttcggaa gtggaaaaat ccacaatcac gtccaccggg
     7441 ttgttcacga aaaactcgtc gttcacattg ccaatccagt ggatcatccc ttcatccgaa
     7501 tccactccgt gcaaccggct cacgtattcc cccatgcggc tggtgctttt gcgcaccacc
     7561 caggaaagcg tgcagcgggg ggagtcgacc atttctgcgg cgaccacggc gcccgtgcgc
     7621 ccgaaaccga acaacccgac tcgaattaag ggtttttgtg tgctcatcaa gagggtttta
     7681 taatcgaata tgcgtgttcc gtcaaaggaa atcgggtaag atgaagatgt gaaatgacgt
     7741 gtgctgactt gaaaaagggg ggagagatga aagaggtgtc tcgccgtcag actttggcgg
     7801 ctctgggtgc gggaacgtgc gcttgcggct ggaatgccgg atgtgccacc ctgtcgaaaa
     7861 aaggaaatgc gccggagatc ccggcggaag cctacaccct tgaaggccgt gcggtcacga
     7921 tacggttgaa ccgggtgcca agcctcgccg ctgtgggcgg atcggtcaaa atcctcgacg
     7981 cgcgcctgcc gcaaccgatg ctgatcggac ggaccggcga aaacgcgtat gcggcggttt
     8041 cgctgctctg cccgcatcgc ggcgtggagg tggaataccg acatgcggac gcgcttttcc
     8101 gttgcgcgag tctgggtcat tcgaaattcc acatcgacgg caagctcaag aaaggctttg
     8161 cgaaaaccgg attgacccga tatgaggcgg ccttggatcc ggcggatccg tcttacctcc
     8221 ggatcacgtt ctaaaatggg gagagagatg aggaaacaag cgctgttcgc ggtggtgggg
     8281 atgatgccgg tgctggcctc cgggtggggt gcggggcacg acacggtggc gcgcgcgctt
     8341 gccctgcgta tgccggagcc gtggtgtacg cacttgcaag gtgaggtgct gcggacattc
     8401 tgtgccgaca gtcattatcc ggattcgttt ctcaagttga gcgaggatcc gcggatcaca
     8461 ccggaggaga aagcgtttct ggagggttgc aaccccaaga tgacggtacg gtatcatttt
     8521 cattcggaca ccggacgtgg tgtggccttt acgctgatgg tgcgcgcgtt gcgcgaaaac
     8581 cgccgggaga gcgcgttgtt gtggttcgcc attctgggac attcgactgc ggatatggtg
     8641 gcctgcaacc atgaccccat tgcgcatctg gcgacgtacg catggggaga caaggggtgg
     8701 gggctccgtt ttccggacgg gacgcctatg gccgcgatgc agtcgggcct tgatctcggc
     8761 tgggtggaga aagaatccgc cgcgaaagcg gtgtgggacg cgcgcgtggc gaacatgtcc
     8821 gcgaccgatt ccggcaagag tgcggatgag gcgcttctgg atgtgatgcg ggccggactc
     8881 gatggggtag gggtctgttc cgcacacggg ctgccgattg tcgaggcggc ggccgagtgg
     8941 acgatgacgc gaaaagccga gtcctgtcag gagcttgcga aacgtctttc agatctgggt
     9001 tgttgggcgg tggagcggct gttgcgtgac tttctcacgg cacagcggct ggctgcgggc
     9061 gatagcgatc cctcgatgac ggaggcggtg cggcagcggt tcaacgtctg gtttgacgag
     9121 tttgtggcta accggaacta taatgaggac tccttcgtga aaggattgac cacagcggtc
     9181 aggcccgacg cgccgctgtt gggcgtgctg tcggaaccga cttggcgcat gaacgagggg
     9241 atgctggggc cgaacgaccg cgtgctggcc gcgcaagccg ccacgtcgct gcgcaagcgc
     9301 ggcaggaacg ccgtcctgct ggatgtgcga cgggtgatgg cggaggggat atccgcagaa
     9361 cgggtgccgc tgttattggt tttcgctcaa aagacttctc cttaccatgg gatggatccc
     9421 aaggttctcg tccgccgcct gattgcgtat cgcgcggcgg gaggcaaact gatatggatc
     9481 ggcggcagcg tgccaaatcc agcactctgc ggtttcccgc aagagcttgt tgcgcaggct
     9541 gatgtcggca aagggtatgc ctacgccttg acgcggctgc cggtcggaac gaacgcctac
     9601 gcgcacctct ccctgaagat cggtgatcgg gcggcgtacc ctctgtcacg atcgccgaat
     9661 ttcagcgcgg ggtggcatgt gcctagcaac aaaacctttt tcaaatccgg ggctgatgag
     9721 catgtcctgc cgattgccca tttgttagac cgcgaccgcg tattgctggt gggtgccgca
     9781 tggccccgaa atcaacctgc tgtcgcgtat cttccggtgt acgtgctctt cccgtacttg
     9841 tggacggacg agtctcccgt gttcacgccg tttgagatcg ggctcgactc ggtggggaac
     9901 gaagcgttgg aagccgcttt cgcggcgctg caagtcgcgc cttaagcttt ataatctgcg
     9961 taatctgtgg accaaatgat tccatggttt attctcgctc cgcctttcgg tttgtcccac
    10021 agcagggttt ccggtatagt gtcgccatgc tacgtaccgg aataggctat gactcacacc
    10081 ggctgacgga ccaccgccgt ctggtgctgg gcggagtcga gatcccgcat tctcgggggc
    10141 tgattggaca ctcggatgcc gatgtgctga tccacgcgtt gtgcgacgca ctgttgggtg
    10201 cggccgcgga gggtgacatc ggccagatgt ttccggacac ggatccccgt tgggaaaatg
    10261 ccgacagcgc ggtgtttcta acagccgtga tcgaacgtct tgaccacaag ggatggactg
    10321 tcgtgaacac ggatgccacc gtcatcacgg aagccccgaa actgcaaccg catcttcccg
    10381 ccatgcgcgc gcggctcgcc gcgctcatgg ggattgaagc agaccgggtg tcagtcaaag
    10441 ccaaaacgaa tgagcgcatg ggggcggtgg ggcgtcacga ggggatcagt gtgctggccc
    10501 tcgccacgat ttcccggcgc gcgaccaccg gttcaagcag gcggaacgaa cgcggagatg
    10561 aatcatgatg caggtgtaca acagtctgtc acgaagcaag gagcctttta ccccgctgaa
    10621 ccccgagacc gtagggctct acacatgcgg tcccacggtc tacaactttg cccatatcgg
    10681 caatttccgc gcgtatacgt ttgaagatct gttgcgccgc acactgattt tcaacgggta
    10741 tcgggtcatg caggtcatga acctcacgga tgtcgatgac aaaaccatcc gcggcgcacg
    10801 gcaggccggc gtgccgctga agacattcac ggcaccgttt atccaggcct tttttgagga
    10861 tctcgcgacc ctgaacatcc agcgcgccga agtctatcct gcggcgaccg atcatctgcc
    10921 cgaaatgctg gacctcatcg ggaaactgat ggagcgtggc ctggcgtacc gatccgacga
    10981 cggctcggtt tatttcagtg tcagcgcctg gcctgcatac gggaagctcg cgcgtctgga
    11041 ccgttccgcc ttgcgtgccg gcgcgcgcgt ttcgcacgat gagtacgaaa aggagagcta
    11101 cggcgatttc gccctttgga aaggatggga cgaacaggac ggggaggtgg catgggattc
    11161 gccgtggggc aggggacgtc cggggtggca tatcgaatgt tcggccatgg ccatgcgcta
    11221 cctcggtgag acgttcgatc tccataccgg cggcatcgac aatctctttc cgcatcacga
    11281 gaacgaaatc gcgcaagccg agggtgcgac cggcaagccg ttcgtcgcgt attggatgca
    11341 ctgcgcgcac ttgcgcgtca acggggagaa gatgtcgaaa tccctcggca attttttcac
    11401 attgcgcgat ctgttgtccc gaggttgggc cggacgggaa atccggtatg tgctgctgaa
    11461 cggacactac cggcagccgc tgaatttcac gtttgaggcg ttggacgcgg cccgcgtatc
    11521 tctggcgcgc atcgacgaat gtgtcgatgc gctgaccgcc gcagcgggcg gctcggcacc
    11581 agcggatgcg ctgccggatt gggccgcctc ggcgcgtgtg gcctttacgg aggcagtcaa
    11641 cgacgacctg aatctgcccg aggcgcttgc ggcgctgttc gcgctcgtgc gggagagcaa
    11701 caccgcgatg cgggatgcgg cgttgacgcc ccagggcgcc gccgctgtgc tggacctgtt
    11761 cgaccggctg gacacggttc ttggggtgat ccgattcgga cgcgcagacc gggacagcga
    11821 gatgccgccg gaagtcgcgg aacgggttcg cgcccgggcc gaggcgcgcg cggcaaagga
    11881 ctggagtgcg gcggaccggt tgcgtgacga acttgcgtcc ttggggtggg aagtgcgtga
    11941 ctcaaaagag ggccagaagg tgaagcggaa cgagacgatg acgcggagta cggcggtgac
    12001 ggcatcatga cgcagaacgg caatacccgg ttgcaaggga agttcatcac gttcgagggg
    12061 ccggaaggcg gcgggaaatc cacacacatc cgcctgctgg aaacatttct gcgcgctcgc
    12121 ggcctcgacg tgaccgtcac acgcgaaccg ggcggcacgc ctacgggtga gacgatccgc
    12181 gcgctgctgc aacatgatca ggcaggggag tcgccggtgc cgcgtacaga ggtgctgctc
    12241 tttctggcta gccgcgcaca gcatgtcgac cggctgatcc gtcccgccct ggcccgtggc
    12301 gaatgggtgc tctgcgaccg gtttgaggtt tccacattcg cctatcaagg gtacgcgcgg
    12361 gggttcgacc tcgacatgtt gcgtaggctg aacacgttcg ctaccggcgg gctgacgccc
    12421 gatctgaccc ttctgttgga tgtgccgccc ggagtcgggg gggctcgcct gcgggatcgc
    12481 catgcgcgaa acgccgctgc cgggcttgac cggttcgaac gcgaggcgaa accgtttcat
    12541 gaacaggtgc gtcgcggttt tctggattta gcccgcttgg cccccgaccg gattgcggtc
    12601 gtgaacacgg atgaggagac ggatcgagtg gcggagcgga tccgcgagac cgttacgaga
    12661 tgcttattgg caggggaatc gcctgatgga tgctgacgca aaattcgcga tggtgcaccg
    12721 agccatcgaa atcgggcatc cggcacacgg atacctgatc gtcgggccgg tacgcggcgc
    12781 cgccctgacg tttgctgaac gcattctgca aaccctcttc tgccgtgaaa ccgtgcggcc
    12841 gtgcggagcc tgcgaagggt gccgccgcgt gacaacgcgg ttggaacccg atattcactg
    12901 gatattccct gaaaaaaaat cccgcatcat cagcgtcgag cagatacggg agaacttgat
    12961 ccgcgaagtg acccagaccg cgctgtccgg aggatggaag gccggggtct tggtcggggc
    13021 cgaccggatg aatgatgcgt ccgccaacac gtttctcaaa acgttggagg agcctcccga
    13081 aaagacgatc ttcctcctgt tgtgcgagaa cccccagtct ctgctgccga ctgtggtctc
    13141 gcgttgccaa cggatcgatc tagacctttt ccgcgatctg gaggaaccgt ggaaaagccg
    13201 cgtgctagag atcctggcca gtccgctttg ggggcagacg ctggagaatc tggccttgtc
    13261 cagcgcactc gcggccatac ttggcgaaat aaaagaggag gcggagcggc gggtacgcga
    13321 ggaaacgaaa cagagcgcgg caatggtgga ggaagacgac gatgtcctcg aggcccgtgt
    13381 gagttcgcgt taccgcgaaa tgcgggcgga ttttcaactc acgctgcttc gctggtatcg
    13441 cgatctcatg gttttgcgtg ccggaggaga tcccggggtg gtttttcaca agccttggct
    13501 ggaggtgctg cgtgagcggg cggcccgtct gcccctggac cggacctttg ccaatctcgg
    13561 gttcattgag gattcggtgc gccagatgga gcgcagcatc ccggatgata cggttctggc
    13621 gtatacgttg gaccgcatcg atcatggcgt agtctaggaa atgccctatg ttgactctcg
    13681 cacgcatacg gttgcctgaa aacagcgttt atcaatgtcg tgtgcccgca tcggtcacgg
    13741 tggtgccggg ggaagtatgt ctggctgagt tggaatacgg actggactac ggtacggtgc
    13801 tggatctgac cggacacacc gagctaggcg agggggaggg gaggggcccc gcctttcgca
    13861 ttgtgcgcaa acagggtgcg gaagacgtgt cgcggctaaa cgagaacgcg cagttggccg
    13921 aaaaggcgac acaggcattc cagctttcgg tgagccgcga gaaagggcat gtcaaactgt
    13981 tgcacacgcg gttttcctac ggtcgggacc gttttttcat ccgctatacg gcacaaatcc
    14041 cggtggacct gcgtcggttt gtgggacaga tccagcggga tttcaaaacg catgtggatc
    14101 tgtggcaggt cggaatccgc gatgagaccg cgcttctggg ctgtttggga tcgtgcggtc
    14161 gcgccgcctg ttgctgcacg tggcagaaac agtttgagcc ggtcaacgtg ctgatggcga
    14221 aaagtcagga gatgtcgctc aatccggtca ccctcaacgg cacgtgcggc cgtctgaaat
    14281 gctgcctgcg tttcgagtac gaacagtatt gcgaatacgg ggaatgtctc ccgccgttac
    14341 gaagtgtggt gacattcccg gccgccgatg aaacgcggac ggacgttgaa gaggaggggc
    14401 aggtgatcgg ccgggatatc ctgcgcgggc ggttgacggt caggacgcgc gacggacgac
    14461 atatgaccgt gtccgcaaaa gcggtgaccg ccaagcggaa tcgagagcgg gaatacggaa
    14521 caggcaagga ggatgataac gatgcgaatt ctcgtggtga atggaccgaa tcttagtcgg
    14581 ttgggcatgc gtgaaccggc atggtacggg cgggaaaccc tcgagcaaat catggaccgg
    14641 gtgcgtgcct tggcagcgga acgtcatgtg gaagtcgaag acatgcaaag tgattcggag
    14701 ggagaactgg tggccgtgat cggtcgggca cgggaacgct tcgacggggt ggtgatcaat
    14761 cctgcggcgt acacgcatac cagcgtcgcg ttacgcgacg caatcgcggc ctgtgggctc
    14821 cccgtggttg aggttcacct gaccaacacc catcggcgcg aaccctttcg gcatgccagt
    14881 ctgacggtac cggtctgcct cggtcaggtg atggggttcg gcggcaccgg atatctgttg
    14941 gcgctggaag ggctgctgcg tcatctgcaa acggaaaagg aaaaggagtc gaatacataa
    15001 tggcacggaa acaggatgcg gttcggctgt gcgttgtcgg atcgattggc ttggatgata
    15061 tccggacacg gacggccgac cggaaaaact tgctgggcgg atcatgccct tacagcacgg
    15121 tcgccgcttc gttttatacc cgcgccggtg cggtaggggt ggtgggcagt gattttccaa
    15181 agtccttcga ccgccgctgg tccgcgttcg gcgtggatct gaccggggtc cagcgtcaaa
    15241 atggcacaac tttccggtgg gcgtgcgagt atgacgcgga tatgatcgag cggaccacgc
    15301 tgaagacgga actgggggta ttcgcggcat ttcagcccgt tttgcccgca gcctatcaac
    15361 gcgctccctt tctgctgctc ggtaacattc aacctgaact tcaagagcaa gttttggatc
    15421 aggcgaaggg tgcccggttt gtggcactgg ataccatgaa cctctggatc gagatcgcgc
    15481 gtccggcgct gatccgggtg atccggcgcg cgaacttgct gaccgtgaat gacggggagg
    15541 cgcgtcagtt gatgggaaaa tggaatctga tcgagtgtgc ggcggcattg ctcaaaatgg
    15601 gtccgtcgta tatcgtcatc aaaaaaggcg aacatggcgc gatgctcttc tcgaagcgcg
    15661 ggatatttct ggtcccggcg tatccggtgc gccgtctggc ggacccgacc ggtgccggag
    15721 attcatatgc cggcgcattt atgggttatc tggcgcgcgc cggacgtgtc agcgaacggc
    15781 ttttgcgcga ggcgcttttc cacgcgagcg tcatggcctc gttcggggtc gagggattca
    15841 gtttcacgcg gttcgaacgt ttggatcaca agacgattga cgcgcgggtc cgtgaactcc
    15901 gtaacatgac cgcaacatca taaaaattgc ggtgctgtct accggtcttg cttcctctgt
    15961 ctctgcctgc cgccaaactt gacataatat atattatgcg acgttattgt ttgggcggtc
    16021 atttacccca aaacgggctg aaatcgctag tccggattcg gccggaccgt ctgtcctgta
    16081 cgtgccgcct catagatcgc ctcaatcaga gccacaggac gacgggctga ttcgccgcag
    16141 atcgcaaagg gtgttccgtt ctggagggat tcaaggaacg cgcccaagca tgcagcgtgc
    16201 aaagcgtgcg acatggcacc gggttgggcc gcgcctgcgg ctgtctcaca aggagcgtgt
    16261 gaaacccacg aggagtccga atcgcgcgct tcgcggaagg aaaaccgtgt caggcgatca
    16321 tcggtcagga ccgccgtgcc gtcggtgccg ctgatttcaa gccgctttgc ctgtcctggc
    16381 caggctgcgg tcgtgccgta caccaccccc acggccccgc cttcaaaacc gagcgaggcc
    16441 accgcactat cttcggtttc aattccggga tggccgacag cggaaacatg cgcgcttacc
    16501 gagatgaccg ggggaagcag gtcgcacaac agatcaatca tgtggatcgc ctgattcatc
    16561 aaagcccctc ccccatcccc tttccacgtc ccgcgccaag acgaatcatg gtaatatgcc
    16621 gcatcccgcc accatggcac atatgccccg gcataggtga gcgtcccgaa gcgcccttcg
    16681 cgaagagcgg cgcgaatcgg ttccagcgca gacatgtacc ggagttggaa aatgcatccc
    16741 aggcgtgtgc cggcctcgcg atgcgcatgg atcatggtgt cgatccgagc cctcgagatt
    16801 tccagcggtt tttcgcacaa aacatgtttc ccggcgcggg ccgcggcgat tgcggcatcc
    16861 agatgcgcgc cggaaggcgt cgcgatcatc agcacatcca actcgctccg gcaaaccaga
    16921 tcctccagcg aggaaaccgc ttcacacccg tatgttgacg caaacgccgc cgtacgcgca
    16981 gcgttccgtc cgcagaccgc gaccagacgg gcaccgggcg tttccgccac ggcccgcgca
    17041 tgaaaatgcg cgatcagtcc cgttccgaca atccccaccc cccatacgtg tccgttctct
    17101 ttcctgtcca tcagacctcc acgactaacg caaaccacaa catcacccaa cgctgctcgt
    17161 ctccttccga tttcgtcgcc atcaatgcgt atcgaggatc atggggcacg cggccggtcg
    17221 ctggttacgg cattcctgtg agatgctgca accgttgcgt cgcgtattgg gctgctctag
    17281 actgcgtgcc gctgcgtctc acaaaagccc gatatgcctg cgcggcttgc ggtttacgtc
    17341 ccatgccctc ataggccaag cctttgtaaa aagaggtatg gggttcaccc ggaacctgcc
    17401 gttcgaagac ttgcagatgg gcgacagctg cggcgtactc ttttttctgc aacgcacact
    17461 gcgccagcat accgtgcgcg cgcgcgccgg caggatacag gcgaaccgcg cggagagccg
    17521 tctgctgcgc ggccccgatc tgcccgctct gtcgttcggc ctgcgcgacc agcatcagcc
    17581 ccacataatc attcggcgcg atccgcaaac cctgctcggc ccgggttttg gctgccgcgg
    17641 cctgcttttg cgcgagcaac gcctccgccg ccgcgaaatg ctggaacgcg gcccgattct
    17701 tccggatgga cgcagtggcc gcgaggaatg cggcctggtt cacgacaccg acgttctgtc
    17761 ccgcatattc ctcccgcaac cgcgtttcgg cggtttccaa ccgttccgtg ctcatcggat
    17821 gcgatgagaa catctgttcg atcatcgaag ggttggaacc gctgatgcgg atgagcagac
    17881 gcatcaggtc caccatcccc tgaggatcat aacccgcccg caccagatat agcatgccgc
    17941 cctgatccgc ctgccgctcc tgttcccggc tgtagctggc aagaaccacc gcgctgccaa
    18001 gttggcctgc ggtccccgcc aaatccgtcc acgcgctgcc gcggctcgcc agatacccct
    18061 gcgtgccgga aaccagcgcg tcatacaccg tacccttgct catggcggat gcggtgtgcc
    18121 cgcaattcac atgagcgatc tcgtgtccaa gcaccgccgc aagctgtgcc tcgttctcca
    18181 gttccgccaa catcccgcgt gtgatggcga tggtcccgtc cggaaatgcg taagcattga
    18241 catagaccgc gttcaccacc tgaaaggaga aaggcatatc cgggaaaacc acatccgaag
    18301 gcttcagcgt ggcgaccagc ctgcgtccga cctgagcgac ataagcattg agttgagcgt
    18361 cggacaccac cccgtaatcc gatgagaatt gcgacggcac ggcctgctgg gacagagaga
    18421 tcaactcact ctttcccaca aggctgatct gctgttttcc agtgatggga ttggtcacgc
    18481 atccggtcgg cgagaccaaa cccaccccca atacaatcag ccctcccgca cggccgagca
    18541 cactccacca ccaagctttt ctcacattca ttgcacacct cctgacattt cgggatctgg
    18601 ctgcatcgcg gcaaccgcag aaaaccggtc accgggctgc ataggatgcc cgttcaaaaa
    18661 ggacttgcca tccatgcggc ggctgccgtc caactgaacc cccgtgagac gcaacggcat
    18721 attgcccgtg accactatcg gtccggacgg ggatatttcg caaatctgcc ccggcacgta
    18781 atcctgcata tggggaaccg ggtcccgcca cacctcggct tggaacactt tcaaacgagc
    18841 cttgttccct tgtttcccgt tcagccgttc cggcagaaag gtgaaggctc cgggccacgg
    18901 gtcgtacgca cggatacgcc gttcgatcac cacggcggag gcatgccagt cgatcagtcc
    18961 gtccgccttt tggattttcg gcgcatacgt ggctgccgca tggtcctgag gctcgcgctt
    19021 caacccgcct tcctgcatca gccgcagcgc cttggccaac gtcaccgcac acagaatcgc
    19081 gagtcgatcc atgagcgtcg ccgccgtatc atccgaatgg atcggttcga tcgccttgag
    19141 aagcatgtcg ccgtcatcca ttccttcagc catctgcatg atggtcacac cggaaacagg
    19201 atcccccgca gcgatcgcgg cctgcacggg ggctgccccg cgatattggg gcagcagaga
    19261 aaagtgaccg ttcacgcaac cgtacggcgg cagtgccagc agtcgtttgc ccaaaaactg
    19321 accgaaggcc actacggcaa tgacatccgg acggagttgg gcgatctggt cgagcacctc
    19381 cggcgcgttc accttttcgg gcgagattat cggccccacc cccttgcggg tggcgtaagc
    19441 tttacagggg cagggtgtga agcgctgacg ccgtcccgcg ggacggtccg gctgtgtcac
    19501 cacgccgaca acgttcagca gcggtaacct caaaatcccc cttagcgcag tggcggaggc
    19561 gtctgatgat cccataaaga cgatacgcat tcacggcttc ctgtagcttg cagaattcaa
    19621 ctattttagc agattaccgg gtgtatggcg cataaaaaaa gcaaggtgtg gaaagacgcg
    19681 tcttcgggcc gcaacctgat tacgcatttg atgcgctttg ccttacatgc cgagtagttt
    19741 tttcaacacg ccgccggagg cctcgcgcac ttcatcgaac aggctccggc cccgggcatc
    19801 catgccgacc accgtctcga ttccgtttac ctcgaaatgc cacatcgctt ccgtggcacc
    19861 gaattcttcc agaaaactga ccccgtccac acggctgacg cgttgtgcca acagtgtggc
    19921 cgcccccccc accgcctgaa ggtacacgca gccgaaccga acgcaagcct cgcgggtctt
    19981 gtcgcccatc ccgcctttgc cgatgatcgc gcgcacaccg aagcgttcga tcagcgtggc
    20041 catataaggc tcttcgcgta tggaagtggt cggacctgcg gcgaccaccc gccacccccc
    20101 gttttccggc accaccacag ggccgcaatg gaacaggacg ccatcccgca acgataccgg
    20161 acaagcgcca ccttccataa gaaacttgtg cagacgatcc cgccccgtgt acacccgtcc
    20221 gcagacacgc actccgtcac ctgcgtttaa tccgcgcacc gcggcctccg aaagcggagt
    20281 ctgtaatatc gtctccgtca tgatcgtcat cccagccact cccgttcccc cccgcggccg
    20341 caccgtatgc cgcgtcggcg gcatgcccag cagaggtagg ccaccgtcac gaaataggag
    20401 gcgggcagac gcgtccgcgc cgtaattttc acgcccagca atgtggtccg cccccccata
    20461 cccattggcc cgataccaag ttgattggcc tccgtcagga cacgccgttc cagcgcggcc
    20521 aactcgggga ttccggcctc atcatccaac ggtcgcagca actgctgttt ggcgacccgg
    20581 tagccttccg cacggtcccc gccaacgcac acgcccagaa tgcccggggc gcaaccaaaa
    20641 ccttgcgtct gccacacagc ctgaagcaga caccgacgca ccccttccat gtcgcggccg
    20701 gcccccaatg cctcgtccgg aagcgagaat tgcgtgctca tgttttcgca gccgccgcct
    20761 ttttgcaaca accagacttc cgtcgtctcc gtatcggcct gctcaatgtg gcagaccgga
    20821 cagtcttccg cgacattttc atccaccgaa gagcctgtca gggtgtcgat cgtattacgt
    20881 cgcaaccagc cgttgcgcgt ggcgagcgcc actgcctcgc gcgtggcctc ttccagtgcc
    20941 aaggtatcgc tttcacgcgg cacccgccag aaaaaagtca gggttccggt gtcttgacag
    21001 agcggggtgg actgcgcacg cgccaaccgc gtattctctc cgatcgcccg caacagccgg
    21061 gccgcctgag agtgcgcggg ttccgccgcc tcggcggtac gcaacgcagc ctccacatcc
    21121 cgaggcagat cgcttgtcgt gcgtcggatc aattccgcga gggtctccgt ccaatccata
    21181 cccatccttt tttttgcggt gtcgcgcagg gttaatccgg cctgaccacc caggcgctgt
    21241 cggacccgat cgcgttcagc aatggcggca gataggcatc cggcacatgc aacgatccga
    21301 accccgttcc caaacggatg tcggacttgg ccagcccgtg ataatcagag ccgccggtcg
    21361 gcagcagatc gaggcgcttc gccaaccgca tcagcgtgac cgtctgttcg cgcgtgtact
    21421 ctgagtacaa cacctccatg ccggtcagcc cgtacgcctt caattcgcgc agaccggtct
    21481 ccaactccgc ttcgcgcaac tcccaggaga agggatgcgc aatcaccgcc acccctccgg
    21541 cttcgcggat catgcggatc ccctcctcgg gatacaggcg gtaccgatcc gcataggccg
    21601 gtgcgccctt cccgagatac cgctcgaacg cctcggaaca ggaagagaca taactgcggt
    21661 cgatcagggc ctgcgcgaaa tgcgcgcgcc ccaccacatg ttcgtccgca taggccagca
    21721 cgtccgccca ctccaattcc agccccagtc cattgagctt ttccagaatc tgctcgttac
    21781 gccaagcgcg gccttccaac acccgatcca aactctccac caccaacgga tgagtagggt
    21841 tcaggccata tcccagaata tgcagttcgc ccggcccctc atcgagatgc gcactgattt
    21901 cgatgcctga tatcccggtc atgccgttcg cgcggcaggc attcagaaac tcctccgtgc
    21961 cgtccagcgt atcatggtct gtcagcgata gagccgtcag cccggcctga caaccgctga
    22021 tcaccaattc ttcaggcgta tccgaaccgt ccgaattcgt tgaatggaga tgcagatcaa
    22081 tcataatcgg tcgcgtacct catacgcgct tcgggcatcc ggctccgcga catccgcgaa
    22141 aaaggccgcg atcgcctgat catagcccgc cgtatgggcg aaagcttttt tcatcagacg
    22201 cagccgggtg ccgaacccca gtgtgccgcc tgtccggcgg agttcctcca gcaacgccgc
    22261 ataatccgcc ggatcggtca ccgaggccac acgcagatag ttcttcgccg cagcccgcac
    22321 catgcagggg ccgccgatgt cgatgttcgt gcgggcgtct tccggggtca ccccggcgcg
    22381 cgcgacggtc tggctgaaag gatagagatt cacaaccacc atgtcgatcg gttcagcacc
    22441 gacgcgcgaa agatcctgcc ggtgcgcctc gttgtacgtc tcgctcaaca agccgaggta
    22501 aattttgaag tccagcgttt tgaccagccc gccctgcatt tccggttgcc ccgtatactc
    22561 ggaaaccgcg accagatgcc gggcggccac tgccgggtca agcatatccc gtacggccgc
    22621 aaaggtgccg cccgtggagt agatcacgac gtttggacag gtctccagca agccgggaac
    22681 caaaacgtcc aagccgctct tgtccgaaac actcatcagc acgcgacgca ccgcaacccg
    22741 gttatccaca tccctcactc gattcaaagc catacgctct ccttctccca acgcttgtct
    22801 gccatacacc tgtagcatag aaagaaggcc gacttttggc aacagcggaa tgacgggaag
    22861 acaaaaggct tttccctcca taagcgggtc tgttatgtta cgtagtgaaa aaaggggagg
    22921 tttatgatgc gtacggtttc aagaatgatg ctggggatgt tggccatgat aacgtgggct
    22981 gaggcgggca cgtccaaaac atttcacgtc tcgcccaacg gctgcgatac agcgtccggt
    23041 tcgtggtggc gtccgttcgc cacctttgaa cgcgcgcaac aggcggtagc cgaattcaag
    23101 gcacggcacc cggctgaagc gattgaacgg attgaggtcc gtttccgcgg cgggacatac
    23161 cctctcaagc aaccgatccg catccgtccc gaacacggcg ggaccgcgac gattccggtc
    23221 atttaccgat ccagcggtga tgcgcccccc gtcttcaccg gtggacgctt gatcacaggc
    23281 tggaccgtcc gcgccgacgg cgcatggacc actcacctgc ccgaagtgaa gtccggctcc
    23341 tgggatttcg cccagctttt cgtgaatggt gcgcgccgat tccggccgcg cattccgcgc
    23401 aaagggtact tcaccacgac caatgacttc gaaacggtcg aggccggtgt gaacggcttt
    23461 tattacaagg aagacgggct tgatcccgca tggtccaacc tgcaggacat cgagttccac
    23521 accctgcaca tctggtccgc ctcgcgtgtg cggctgaagt ccatcgacgc cagccgaaaa
    23581 atcgtgcggt ttctgaagac ccgcaactac aaccagtact ggggaagcta taaaggcccc
    23641 ggctggggag aaaacggctg ccggttctgg gccgaaaacg tgaaggaagc cttcggcgtg
    23701 cccggcgagt ggtaccttga ccggcctacg ggaacgctct cctatatgcc gatgcccggc
    23761 gaacgtcccg atacggcccg tgtcgaggtg cccattctcc cgctgcttct gatcgtcgag
    23821 ggcgacgcgg atcgccctgt catcaacacg cggattgagg ggctggtttt caaacagacg
    23881 tgctggacca cccccgagtc cggaaatttc atcccccaag ccgaggtcaa catccccgcg
    23941 gccgcagagt tcgtcaatgc ccggaacgtc acagtcagcc gttgcgtgtt cacccaactc
    24001 gccggttaca ccttcgcctt cggcccgggc tctcactcca acacggtcga tgcctgcatc
    24061 atgtccgacc tcggcgcggg cggcgtgaag atcggccctc catacgcggg ctatagcatg
    24121 ttcgagagca tgcggaacga tttccaaagc aagaatgaac tcgccgacat ggataagact
    24181 tcggccatca cgatctcgaa ctgccgcatc gtcggcggcg gacgcatcca tcccgcagcg
    24241 cacggcgtct gggtcggcca tgcttcccac aaccgcatcc tgaacaacga gatcggcgac
    24301 ctgtattaca cctccgtctc gctgggctgg acgtggggat acgcggagcc gtcccgcgcg
    24361 caccacaacg aggtcgcctt caaccacatg caccacatcg gccaaggcgt cctttccgac
    24421 atgggcggcg tctacaccct cggcgtttcg ccgggcacaa ccgtccacaa caaccgcatc
    24481 cacgacatca atgcgttcac gtatggcggc tgggggctct ataccgatca gggctccagt
    24541 tatatccaca tgtacaacaa cctcgtgtac aacaccaaga ccggcggatt cgaccaacac
    24601 tttggccgcg agaacatcgt tgagaacaac atcttcgcca atgcgcgcaa agatcaggtg
    24661 cagcgctcac gcgacgagga acacatctcg ttcttcttcc gtaacaacat cctctactgg
    24721 gagactgacg gaaacctgtt gggcaacaac tggaaaagcg gcactcgcgg catgaaggac
    24781 ggcaagccca cccagcacta cgaactcggc ccgaacctct actggcacac ctccggcaag
    24841 caggaactct tcccgggcaa gaaaacgcta gcccagtggc aggccgagac cggtcaggac
    24901 gcgggctcgc tcgttgccga cccgctattc gaaaaccccg ccgcgggtga cttccgcctc
    24961 aagcccggtt cgcctgccac ccaagtcggc ttcaagccct tcgacaccaa cgcggccgga
    25021 cccgacaagt cagcccgcct cccgaaattc aagctccgcc ccgtccccac catgtacgaa
    25081 atcacgacgc cctgaaccgg tcatcatgag tatctcctat tgcctgaaca ttcatcccgg
    25141 cgaaagtctg gatgccgtgc gccaggcgat cacgcgccac gcgtgcgccg tcaaagcccg
    25201 cgtctcgccc gccgcgccct atccgctcgg actgcgcctt tccgccgcag ccgccggaga
    25261 gctttccgat cccgccgcgc tcgctgcatt caccgacctg ctcggctgta acgacctctt
    25321 cgtgaacggc atcaacggct tcccctatgg cacgttccac aacaccgccg tcaaaacagc
    25381 ggtctaccag cccgactggt caactcccga gcggttgacc tacacaggcc gtctcgccac
    25441 ccttctggcc gcgctactgc ctgaaaacca gaccggcaac atctcgactg ttcccatcgg
    25501 gcacaaaaca accgcccaac ctgcaacctc atcccttccc gtccgccaga tcacgataat
    25561 ggcggagttc ctgcacgacc tcaagcagcg cactggccgc gaggtcgtcc tcgcgctgga
    25621 gcccgagccc gactgcctgt tggaaaacac gcaggacgtg atcgtctggt ttgaggacgg
    25681 gctactgacc gaaggtcggc gctggctttg cgccagcaga cgccgtacgc cggaagaggc
    25741 cgaatcgtta ctgcgcaccc atgtcgggct ctgcctcgac acctgccatt tcgccgtcga
    25801 attcgaggac ccgctcgccg cgcttgcccg cttcgaaagc gcaggcctcc gtgtcgcccg
    25861 tatccaactc agcgccgcca tccgcgccgt catcagcgat gcatcgctcg cgcggctcgc
    25921 cgacttcatt gatccggtct atctgcacca aaccaaaatc cagctaccgc gcggcccgct
    25981 cctttccctg ccggacctga ccaacgaaac gctgatgact gcccgccgac acatggggcg
    26041 cgaactccgg acacacttcc atgtgccgct gttttacgca ggcgacggcg tcctcgactc
    26101 cacccacacc gacctcacac ccgaattctt tgcgcataca gttgcgcaag gctatccgct
    26161 ggagatcgag acctacacct tcaatgtgct gccttccgcg ctccgtcccg cctcggtgat
    26221 cgacagcctc gtcgccgaac acgattgggt cagtgtacgc ctgcatcgac accaccagca
    26281 ggaaagactg tagtcgcgtc gcatagggca tgacatctca gcctcgcccc caccccacac
    26341 agccgctatt tcaattcacg cgccccgcgt ggggcgcgac ggcggccaac cgacgcggcg
    26401 tcacggcgag gaggtttcaa ttcacgcgcc tcgcgtgggg cgcgacggct tgacctcgac
    26461 tgtgttaata aacttctcgg tctcgaggta atccgcgtcg acaagtgccg aaaacagcat
    26521 ccgaacgtcg agcatgtcgg agcaacgttt acccgtccga ctgtaaaacg acttctgtaa
    26581 ggtaggccat gacaacccgt cttctgcgaa cagttgtaac aattcgttca tagagtccgc
    26641 agatagtgtt gtatcggggg aaggcgcgcg gctgcaaatt ttcttgaagg attccttggt
    26701 gtcttgttgt aatccgacat gatgcccttg tgcaattaaa gaaagacagt agcctgcttt
    26761 tccatactgc ttcattgcgg tccacgcacc agcagtccag tggtcgatgt gccgcccctt
    26821 accatgtaat cgttcttgaa accgtatccc gtacttcccc aagtcgtgga gcaggccggc
    26881 aatgtacccc tcgacgcctg caccaaagat ggaggcgaat tggcgggcgc gagcggccac
    26941 gttcagcagg tgcgttttaa gcggctccca atcttcctgg tgtcgtgccg tgtgtgcaaa
    27001 acattttctc atatccccct taattaaaat cgcccgatca gtagccagca caatactggc
    27061 actaccgacc gggagattta ttcagcgttt aatgttcgat ctttggtttg ttttgcgttc
    27121 aacgggtggc ctcccccatc gtcccgcatc ccctacaccc attttgcctg aaaggcgact
    27181 ttgggggttt cccgccccca ccctgcacgg atccgcgcgt cgagcgccgt ccaagtcggc
    27241 acgccgctcg tggcgtccgg ttgttcgacg cttgcagaac ccaccgctac ggctgtgcgc
    27301 aaactctccg ccggggtgag tcccttggcg attgccgcca ataatcccgc caccgtgcag
    27361 tcgccggaac cggtcgtgcc cgcgaccttc gcgtcgaaac agggcgcgaa aaactccaca
    27421 cccccccagg ccgtacggga tgcggtgtca aaacatccgc atgtcgccag ccgcgtttcg
    27481 tctgaggtca cccgcagata agctccctga ttgcccagct tgagcatcac caccgccacg
    27541 cctagcgcga tcagttcgtc agccagcgca cgcacgtcct cttcgatcag tccgcccatc
    27601 ggcacgccct cagccgcctg ctcgcgcact ttcaatccgc gctcgcggtg cagcatgaag
    27661 acgatctcgt cgaggctcgg cgagaagatg tccgtaaacg gcagcgcctg cttcaaaatc
    27721 gcgcgccaat ccgcctgccc ggcgggcgac tgcgggtcgg gcagcgccat gtccagcgag
    27781 gtcgtcagtc cgcattcctt gaccttcctg aacaggcgca tcagttcgtc cgtcttggtg
    27841 tagaacgccc gcatcagggg cgggtagccg aagtggaaga tatccatccc gtcgagaagt
    27901 tgcgggatca catcgtccgc accgaaggtg tcgttcgcgc ccggacagtg caggaagatg
    27961 cggtcgatcc ccggcgggtt gatcaccacc gtgtaggagg tcgcgaccgc cggatcgacg
    28021 atcatggttt tcaccagtgt ctcgtcatgc gtccggaaaa catcgcagac cgccgcgcca
    28081 aaggcgtccc ggccgacttt gcccatgagg cgcacgggga ggccgagacg ttttaaggcc
    28141 atccccgtgt tagccaccga cccgcccgtg gagagcaccg caggacccat gtcgaccagt
    28201 ttgccgggct gcatgaccgc ttcgagcgtt ggcgcaccgc gtagaaaggt gggaatgata
    28261 tccaggcata catgcccggc cgcaatggtt ttcaacgtcc gcatcgtttc tcctcgtttc
    28321 ggatcccgca cggatcggtc agtgcatctc gacggcaact tcgccgatac cgctgcgcag
    28381 gtagttgaac tggacattgg gatgatccgc cagaagcgac atcggcacgc tgctgtccgg
    28441 gatgcgcttt gagatcatca gcgcggagag ccggatgccg aacgggttgt cgtggtggcc
    28501 gggatgccag atcgaaacct tggccgactt ccacgtctcc accggaccga ctgtcgacgc
    28561 gctcgtcggc accagcgcga cgttgccgcc gccagaggta cgcgcgttct ggatgaccgt
    28621 gacgggatgc agatcaacga gacgcgtggc gagcttgcgg tactcctccg gtgacggcgg
    28681 cgcatccgca taggcgcctt cgcgcttgac cgggtcattg aaggcccagt gtttgatctc
    28741 tccctgaccg ccctgcatca gcaggcactt gatctcatcg aaggaacggg tgaaggccgc
    28801 aagatcacca gtcgggaagt gcatgttccc ggcgggcatg ctcaactccg gcttgatccg
    28861 gtcgaaacac atctcgcggt cgcacttaga gaaggagagc ggatgatcgg tcgagacgat
    28921 tttgccgttt tcgacccact cgtccatccc ccagaaatgc gcgtcgcgca ggttcaaccc
    28981 gatcgcgttc acgatgcggg ccaccagcgg cagatgctca gtcgggccga tcgggccgca
    29041 cacgccggtc gggttatcgg gcgtggcctg acgccaagcc tcgatatact ccaacgcttc
    29101 ggccgcgtaa aactcctcga ccgtgtcgta gatgcaaacc ttgaatcccg gacgcgaaag
    29161 ctgctcaaga tccttggcgg tgagcgccgc cgcatcgttc aggatctcgc tgtccagtgt
    29221 cgtgtagtcc caccatccgg gggccagttt actgattgct cgtgccatgt catttcctct
    29281 ctttcttccg ttttggttgt tgaagttctc acctacccgt cacgttgacg gcgcgacacg
    29341 ccgcaacagc ggggccagcg ccgtctccaa cggcgcgaag tccggcgcac agtaccccat
    29401 gtgactatgc cggcgccagc cctctgcgaa ttcgcagccg ccataccgtc tgccgacttc
    29461 gcgggccgca tccgccatac tctccacata cgagcccatg ccctgcgtcg catcgagcca
    29521 ttcgcgctga ctggtgtgac aagccagcat ctcgcgcttc acctccatca cgcttccgat
    29581 gtcgatgcag aactccggct cgaccggtct gcacagcatg tccatcaggc tgtgcggcaa
    29641 cgaatgatag accgccaccg gcttgttggt agacggcagc ggcggatctg tcacgtagct
    29701 gggcagacaa cggttgaatg cggcggacgc cgcaaggcgc gacgcatact cgtggtcttc
    29761 catataatcg taaagcgaga gtgtcagcag gatgtcgggg cagaccttgc ggaacacggc
    29821 ggaagccttg gctagcgaat cggcattata gaaaagcccc acatcgtcga agagtgacgg
    29881 atgccagacc gcgccggcca cgcgtgccgc ttcctgcgcc tccaatgccc gcacgcgcgc
    29941 cgcttcctct ttgccgtaaa cctgtgaacc gtagcagccg ttggccagat tgcagatatg
    30001 gatctccgcg ccggcctgtt tcagcaaaag cagcgttccg ctcatcatga actcgatatc
    30061 atccggatgt gccgccatcg ccaacgcgac cattttctca ctcattttcg ttctgacctt
    30121 tcccctcttt ctgcccgctg cgtttcagcc gcgtgtcaca atcacctcgt ccggcgccac
    30181 ccagacctct gccgggtcac ctgtccggtc cgttccgccg gaagggttca actgaaacgc
    30241 cgtcagcacc tcacacggcc cgtcctccgg acccaaatca atctcatgcc gggccatctc
    30301 gcctagataa acactcgcac gatagcggcc gcgcatccgg ttcattccgg cgtgcggcgg
    30361 cacaccgaac cgcagcgttt cgggacgcaa acagaccgtc acctcctcgc ctgtccgaat
    30421 ccctcccggt gcgcggcggg cttggaacag cccgatccgc gtcgcgacct gcaccccctc
    30481 ggtttccgaa acggactcaa tgcggcccgt gaggaaatgg ccttcaccga tgaaccccgc
    30541 cacgaaccgc gtggcgggat gacggtaaat ctcctcgggc gttccgatct gctcgatgcg
    30601 accgtcccgc atcaccgcaa tccggtcggc gatggaaagc gcctctttct ggtcatgcgt
    30661 cacatacacg gcggtggtcc cagccgcttt gcagatgcgc cggatctctt cgcgcatctc
    30721 caggcgcaat ttcgcgtcga gattcgagag cggctcgtcc agcaacaggc actgcggctc
    30781 caccaccagc gcgcgtgcca gcgcgacgcg ctgctgctga ccgccggaaa gcaggttggg
    30841 acgcgctttg gcgcggtctg ccagatgcac gagttcgagc gccgctgcca cgcgcgcgcg
    30901 gatttccgcg cggggccgct tccgcatctc cagaccgaag gcgacgttct ccgccaaggt
    30961 catgtgcggc cacagcgcgt aactctgaaa catcattccg gtattgcgcg catgcggcgg
    31021 caggtcggtc acatcctgcg tcccgaaaaa aatccgcccc tcatccggag tgtgaaatcc
    31081 ggcgagacag cggagcagcg tagtcttgcc gcatccgctg gggcccaaca gaaagaacag
    31141 ctcgcccgga cggatcgaca cgctgacccc gtccagtgcg cgaactgccc cgaacgtttt
    31201 catgatccct tgcgtttcga ccccgactgc catgtcccgc tccgaccctt cccgtagccc
    31261 catagccaac ctagcttttg tggtttaagt atatcgattg tcacggcaca gggcaagcat
    31321 ccagcagttt tctgcggaaa tcgatcccgt tccgttcttc catgaggctt tcaagtggtg
    31381 tatgataatt gctttaagac acaaaccgga ctcacgccat cccatgacct ttttcatgat
    31441 cctgaaagtg gcgctgcggg ccatcttccg caacaaaacg cgttcgctgc tgaccgcgct
    31501 gggcatcatt gtcggcattg cggcgctcat tgctgtcgtg gccagcggcc aaggcgcgca
    31561 ggccatgatg gtccgcgaaa tcagcagtat cgggaacaac atcatcatgg tctttcccgg
    31621 aagccgtggt gctctccacg gttggcggtc tgatcggtgt attggctggc accttcggtt
    31681 tttatcccgc cctccgcgcc tcccgtctca atcccattga ttgcctccgt tatgaataaa
    31741 cgtatgacga tgacgttgtc ttcctccctc ctcgacttcg gtgacgttcg ggccgcgctg
    31801 gggcgcggtg gccgtgtcgc cctgttcatc cgccattccg aacgcccgcc catccgttcg
    31861 gacgataagg atttcggccg acatctcggg ctgacccccc gtggggtcgc cgtggcgcgt
    31921 gaggcgggcg cgatgttggc gggtgccccg gacgtgcggc ttttggcaag tccgatgcaa
    31981 cgctgtcgtc tgaccgcacg gcatatcgcc gagggcatgg gggttcccga accgcaggtg
    32041 gaagatgcgg acccgctggg tgtgcgcggt tttttttacc gtgaccccta cgcggtgcag
    32101 gagatcatgc gtcagcaggg atacatggct tacatgttgg agtatctgaa gaaggggaac
    32161 gccctccact cggcaccgct catgaccgcc acggaacaga cgctggagtg gatgattacg
    32221 caaatgaccg tacccttcgt tctttttgtc agtcacgata tcttcgtggc cgctattctc
    32281 accgccctgc ggctccgctc ttacacggcg gaggagtggg tcgggtttct gcacgggttc
    32341 gcattgatcc agcacggtca agatccttgg gcgtgccacc catgccttcc gtcgcacacc
    32401 gaggtcgatg cgtcccaccc gtttatccat tgagttccgg agactgtatg ctgatcgaac
    32461 gttttggcct gtatcttgtg atcaccgacc ccgtgacctc atacgagcgc tgtgctgaag
    32521 ccgctgtggc ggccgaactg cgctacatcc aattacgccg gaaaaagtgt gaccgggccg
    32581 caattttggc cacggcgcac aatctgcgca ccatcacccg cggcacctcc acgcgcctga
    32641 ttgtaaacga cgatcccgaa ctggcggcag aggccgaggc ggatggtgtg cacctcggcc
    32701 aagatgacat gcccctgccc gaggcgcgcc gccgttatcc cgtcctgcgt cactttggcc
    32761 tctccacaca taccccggaa caggcgcgct ccgccatcaa ggtcgccccc gattactgcg
    32821 gcgtgggacc ggttttcgcg acgccgacca aagaggtccc cgacgccaca ttggggccgg
    32881 aacaggctgg cgcgatcatc cgtgcggcgc ctttcacaac cgttgcgatc ggcggcatcg
    32941 atgccgacag gttaccggaa gtgctgcggg ctggcgcggt gaatttcgcc gtggtgcggt
    33001 atgtgtgcca ggccccggcc ccctttgacg ccatccgccg tctgcaagac atttggcagg
    33061 cggcccaacc ttgatctctc catgtcccgt gaatacgaaa ggagtttcct gatgatgcgt
    33121 tgcctcctga tcccgctctt gttttacatc ctgtgtcgga caaccgcatc cgcccttccg
    33181 gagatcaacc tcatcaccga gccggcgtgc aacagtgaag tggccgtgat cgtcggacac
    33241 gggttcgttc ccggccggac acgcgtcaag gctttggcgc tcggcggata cgatatgcgg
    33301 aagccgcagg atccgcttag gttcgcgaag gcgatcggcc gtccgcccga tcttcctgac
    33361 acaccaccgc cggaggcgca ggagtgtgag atcgtgggac agggagaggt cttcctgcaa
    33421 gtgctgttcc gctgtctgcc gcaatcgtgg gtgtgcgctc cactcacctc cgccgtctgg
    33481 gtcggggacg aatcagggtg gggccgtccg attctcatca accgtccaca ggcgcaatgg
    33541 ttgtatccgc agcgccaggc gccaggagag ccgatccgaa ttttcggcag aaccttcgcc
    33601 tggggttggc tcctgccctc tgcccatgtc ttgttacggc cggtgggttc cacgcaaatg
    33661 gtgcatctcg cccgcgctgc ccagcatcat gaggacggac acacggaacg ttggtcactc
    33721 tgcgtctggc taccggagtc gctggccgaa ggcgaatacg aggtgtttgt ccatggtcgt
    33781 cacggtgcgg agtacggatg gtccagcccg ttgcgtctga ccgtcgccca aaaaccgtcg
    33841 tcacggccgg tcgtttcggt gcgcgagcac ggggcggtcg gcgatgggct cgcggatgac
    33901 acggcggcct tggaggctgc gctccgtcaa gccgcacccg gcggcacgct ttttttgccg
    33961 gccggaacgt acgcaatctc ccgaaccctc aacatcccgg aagacgtggt gatccagggc
    34021 gcgggcatgc gccagagtgt gatcgccaat cgacaagaac cgtgcgtgga tccatttgcg
    34081 ttgacacaaa cccgacccgt ttttgatgga aaggatctgt tatgggggat gcgccggttt
    34141 gctttgcgtg atctgacctt acggttcatg cccgcgctcg gttcggcact gcgtgtcggc
    34201 cgtgatccgc tgtggtccga agacgtctcg ctctaccggg tccggcttga aacccagcag
    34261 gatttcggac tgtcccccac gcacccctac tgtgcccggc cgctcaacat cgtcaaagcg
    34321 cgccgcttca gcatggtgcg ttgtgagacc tacggaccgg gagcgtgcag ttgcgaacgc
    34381 aaggtcgagg acagcctgtt ttcgcaaaac cgtttcatca ccgatcggcg ctggcgcggc
    34441 cacggtttca aattctgggg tgcggaacag gtgatcttcg aagacaacct cctctccggt
    34501 gacacccgcg gtctggtgat gcagacccat ttcggcgtca actaccgtaa tttcattgcc
    34561 ggcaacacgg tggaacgaac tgttctgggt gagaatgcgg gcgagacgta tctggttgag
    34621 ggggcgggtt atttgggtga gagtgcggtg gaggaggcca ctcccacgca tgtccggatt
    34681 gcgcgctggc caacggcgcg gggggctccc gccacggccg agatgtgcgt gggccgtttc
    34741 gttgtgatcg cgggcgggcg cgggatcggc caatggcggc gagtccgtgc agccgatccg
    34801 gcccaacgtg tcctgtaccc ggaaacgccg tggcgtgtcc cgccagacgc ctccagcgtg
    34861 gtcgtgatga tgaacggcct gatcgacacc gttttcgtca acaaccaaga gaccgattgc
    34921 ggcaagggct tgtacattta cggtgcgggc gcgatcaact gcatcatcga ccgtcacctc
    34981 tgcgcccgca ctctgggcat tacgttcaaa acacatgacg atcggcagaa cgccgacccg
    35041 atgcatcacc aaacggcacc cgactttttt aacctcatcc gtgattgccg tgtgcatgac
    35101 ggcggcggac tcttctgcgc tgtgggcgga cgtctgccac tggaagaacg caacggagtg
    35161 ccttgggcca atttcgccaa ccggttcatc gccaatgaga ttcagaacgt gacccctttc
    35221 agcggtgccc agtacggggc gaactggact tggggcggcg gatgggacaa cgttcaggcg
    35281 ggcatcagtg tgatccccag cgacctgggc actgcggcag ggagcggaag tccgggcccc
    35341 gaacgcatcc gcgccgccac gttccaaaaa aactggattt cgggatcccg attcggtgtc
    35401 ggggtgagcc gtcgcgcggc ggacaccctc ctgatgcaga ccacggttta cgatgtggac
    35461 gtgccgctga cggacaaggg ccgtgacacc cgcgaaaaag caacggttgt gcggacaccc
    35521 gaaacctatt cgccggaacg gggtccgatc cgttgaggaa tccaaacaaa cggttgccca
    35581 ataaaaggta ttccgtttat actgtgcccg tttctgggtt atctgataag gagattcgac
    35641 atgcgaaacc aatggcaaac caagcaactc gcccgacgtt gcgtcatcgc cctccccttc
    35701 cttctgtttc tcgtttcctc cggctgcaaa ccttccgcga aaagagccgc cgaacgcgag
    35761 gcgtacctca agcatgaagt ggagcagacg tccctgcgta ttcaggatta ttcctacgaa
    35821 cggcaggatg aggcgctggt tctggttgac gaggcgttgg ccaaccctcg ttttgctgga
    35881 cacaagccga ttttcttcgc gcacaaaatc aacctgctgc tctcattgga gcgcgtgaag
    35941 gaggcctctg atctggtggt tgaagcttgg aaaaaagcac cggatcaggc gcaagccgta
    36001 ttcggaaaaa tttgcgattt ccatcagcag cgcaacgcat accgcgagat gaacgactgg
    36061 tgcacgcgac tgctggatct cggcgcggct ttcccgaaag agctgagagg gcggttgatg
    36121 ctgcggcggt tgacggccgc gctgggcatc aaggacatgg agcaggcccg gagcgatctg
    36181 gacacccttc tgacgctcgt ccggcccgaa gagtctctgc cggatctgca acgttccttg
    36241 gagtggttca ttgatatggg gaatcaccgc gaagcgcttg tcctgttgaa ccattttgag
    36301 aaggctgccg ccggtcaacc ctcgtaccaa ccgctgatcg tgtcgctgaa actgcgctgt
    36361 atcgtgctga ccggcgaatg gggacgcgtg gtcgaggccc tcgcggcctg tgtgccgcaa
    36421 ctgccggacg cgatgctgca ccagaccctc aagaatgttt tcggcgccct gaataaaaac
    36481 aatcagacgg atctgttgca acaggccgct aaacagattt tgttcaacgc cgtgggcaag
    36541 gaggcatcgg ttcactatgc cgcccgcgtg tgggtggaca cgggtatgac cgtcgataag
    36601 aaagtcctgg cggagcggct caatgtgttg ctcaacgtca aagtttcccc gattctggtc
    36661 ggtgacttgt acgagcggta tttttacgag acgatcgagg accacaacgt ggtccggaac
    36721 ctgtgcgcgg tgggcgaacg cctgctgacg gtctgcacga acgaggcgac ggtcaacatg
    36781 gcgaaagtga aggtgctcga cggcgcgttc attgtcaacg attacgacaa ggcgatccac
    36841 atgctcgaac aaggcatccc cggcaaggat aaagcctggc acgacatgtc cctgcccaag
    36901 gtcaaagcgc accgtgccat ggcgcagaac aagccgcgcg aagcagtggc gtatttccgc
    36961 gcgttcatga atgcctggct ggcgacggat caagacgagg aatccgatcc gactacgggc
    37021 atctcccaca gccgggaatg gattctcgga cgcaacgcca ttcgtatcgc caggctgttg
    37081 gagtccattc cagataagga ggaggcggac aaagcgcgcg aagaggccaa agactatttc
    37141 aggaaggccc tgatcaaagc cgagaatgac ccgccggccc tgaaactgct gaaagaagag
    37201 atccggccgc tcggtctata agcggcgatg atcagcgcgg ggcgcacggc cttcacgggc
    37261 ggggccattc ggtcaccagc cggacgatcg cctccagcgc ctccgcttcg gggagggttc
    37321 tgatccgttc gccccgcaca aacagcacga acacgccgtc gcccccggcc acagcgatat
    37381 cggcaccctt cgcctcgccg gggccgttga ccacacaccc catgaccgcc acatgcggcc
    37441 gcggcgcgtc agggtgttcg cggtaaaaac gttcgaggcg gttttcgatt cgcaacgcca
    37501 gaccggccac gtccacccgc gtccggccgc atgttggaca cgaggtcacc gaagcactcg
    37561 gcgcgcgcaa cccgacgctg cgtaagagtt ccacccccac gcgcacctct tgggccggct
    37621 gatccgtcag cgaaacgcgg atcgtatcgc cgataccctc cagcaagagt gtgccgagcg
    37681 ccacgcagga gcgcactgtt ccgggcagaa acgtgccagc ctccgtcaca cccagatgaa
    37741 gcggataatc ggttcgttcc gacaacaggc ggtaggcggc aagggtacgc ggcacgtcag
    37801 aggccttcac cgagattttc atctcgcggt agtcctcggc ctcgagccac gccacatgcc
    37861 gcaacgcgct ttccaccagc gcctccggtg tcgcggagcc gtatcgcgcg agcagatcct
    37921 tttccagcga accgccgttc acaccgatac ggatgggaat gcggttcgga cgggccgccg
    37981 ccacgacggc ccgaacccgt tcaggaccgc cgatgttacc ggggttgata cgcaacccgt
    38041 ccgcgccagc cgccagcgcc gccagagcca accggtgatc gaaatgaata tccgccacca
    38101 gcggcagcgg ggagcgcgca cgcacctgtt tgaaggtttc ggccgccgcg tgatccggca
    38161 cggtcagacg caccaagtcg cacccgagtt ctgcggcttc acacacctgg cgcaacaggg
    38221 cctccgcatc atgcggatcg gcgttcgcca tggtttgcac actcaccggg gcaccgccgc
    38281 cgatggcgac ggaccccaca tgaacctgac gcgtcgcagt acgcggacac cgcatacatc
    38341 gtcctcctca tcaacactgc ctaccgcggc acgaccgatg cgttggtctc ggacgccgtc
    38401 tctgcctcct gacgcgcctc gcgaaccgcg gcacggaccg cccgcgagat tttgacctga
    38461 cgcgcgatat cgcggtaaaa taagagcacc atcagtccga tcagtaaaaa cgcgaacgcg
    38521 ttcaccagcg ccgaaacgac tttcgggtgc ggtttacgac gggtggtcac ttcatacaac
    38581 gcgaacagga tgtgcccgcc atccagaacg ggaaacggca gcagattcag gatcgccaga
    38641 ttgacgcaaa tcatccgcaa gaatccgatg gcgtcaagga tatcgccccg taccgtgtga
    38701 aagagcccga tcacgatggc caccggtccg cccacgttac gtgccaccgc tttacgctcg
    38761 cccttcgatt ccggcgccag cagggcctgg agcacgcgca ccacggacag ggagtcccac
    38821 ttcaactggc gccaaggatt acggtacatc atccaggcac gaacccgttc cgtggcgtct
    38881 ttccagacaa tgccgacgag ataccttccc gaggcctcgt caaaacgggg tgtcagcacg
    38941 atctgctgga gttcgcggcc ccgtttcacc gtaagcgtca cgggttgcgc ccggttgtgc
    39001 tggatcgctt cagcgaactg ctgggcctcc cgcaccggct ctccgttaat ctccaccacc
    39061 acatcgttcg ggagcagtcc ggcttgaatt gccggcatgt cgggcatgag cgccgagacc
    39121 tcgcagtgcg ttgcggggaa gaccccttcc agcatcttca gttccacaat ctcgctggtc
    39181 cggaatggca gttccacctc ccgcaccgtg tccccgcgct ggatgccgaa cacggctttg
    39241 ccggaatcgc ccgcaagttg gcactccacc tgcaaatcgg accaggttcc caccttccgg
    39301 ttgttaacgc tgaggatccg gtcccccgga cgcaccccgg ctttccaggc ttccgagttt
    39361 tccgccacag aaccgatccg cgtatccagc acgcctgttt tcacgcccgg agccagaaag
    39421 aggaaatagg ccaaaaagat ggcgagcacg aagttcccga ccggtccggc caacgcgacc
    39481 acaatccgtt tccacggggc gatatcctcc aactccgaac acgaatcccc ctccccgtcc
    39541 cgctcgcttc cctgcacctt ctccatccct gaaggatcca attgcggcag ggccacatat
    39601 ccgccgaacg gcacgcatcc caccttatac accaccccgc ccacggtgcg cttccagagc
    39661 gcaggtccaa atccgattga aaaggcatcc acacgcaacc ccagtcgccg cgcagcgata
    39721 aaatgcccga actcatgaat aaagatggcg aggctgaaaa acaacgccac gcaaacaccc
    39781 gatccaatca gactgaccca ttccatgctt ttccacctat ctcacgtcgg cccgatacgc
    39841 cgcaaccggt atgccgcggc gcggcgcaca ctcacgttgt ccctgatttc ttcacctgtg
    39901 cgcccgcctg gcggcgcgcc cacgcatccg cttcgagaat ggtttccaac tcgacacagg
    39961 ttttcacatc atgttgcgcc atcacagcct ccaccgcccg ggcgatgccg gaaaacggaa
    40021 tcgccccggc caaaaaggct tccaccgcca cctcgtccgc ggcattgagc accgcaggtg
    40081 cagtcccccc cgcctgtgcc gcctcgcgtg ccagccgcag acagggaaac cgcgtcgtgt
    40141 ccggttcggc aaaggtcagg catcccatcc gggccaagtc cagacggggc agatcggcag
    40201 tcagccgttc cggccaagtc agggcatact ggatcggaaa gcgcatatcg ggcatggaaa
    40261 gttgcgccag cgtggcaccg tccgtaaacg tgaccatcga gtgcaccacg ctctctggat
    40321 ggatcagcac atcgatcttg gcgaccggaa gattgaacag ccagtgcgcc tccagaatct
    40381 ccagaccttt attcatcatc gtggccgaat cgatcgtgac cttccgcccc atgtcccacc
    40441 gcggatgggc cagggcctct tcaggcgtga cgcggtcgaa atccgtttct ttgctcgcgc
    40501 cgaacggccc ccccgacgcg gtcagcgcaa agcgcgcgac acgcgcttca atgcgggagg
    40561 tgtctgcggc atcccgtaca caggccggcg gacaccccgg ggtttgcagg cattggaaca
    40621 gcgcactgtg ttcactgtcc accgggagca tccgcacacc agcggctgcc cgccgccgca
    40681 tcaccaattc gcctgcggcg accagcactt ctttggtggc cagcgcgata tcgtgcccca
    40741 ctcggatcgc ctccagtacc ggttcgaggc ccgccagacc gaccaaggcg cacaggacca
    40801 tatccgcctt cggcagcgcc gccagcgccg ccactcccgc ttcaccgggc cagacctcaa
    40861 tcccgtgcgg acgggccagc gtctccgcct cccgcgccgc gttttcatcc gcgaccgcca
    40921 ccaccggcac atcaaagcgg agggcttgat ccaaaacacg catgatgtgc gttcgagtcg
    40981 agagccccgc cacacgcaac gcttcgggat gcgcctcgat cacgcgcaaa gcgttctcgc
    41041 cgatcgatcc cgtgctcccg agtataacca ccgacctcat aacgccaccg actgtcccac
    41101 aaaccaaacc agataaaaat agaggactgc cggcgtgaag gtcaggctgt cgaacatatc
    41161 cagaatcccg ccgccgccac ccggcaacag gccagcggaa tccttggcgt tcaccgaccg
    41221 tttgaacatg gattcaatca gatcccccag tgcgccgacc acactgagca ccaaacccaa
    41281 aaccagcgcg tgggtcatac tcaactccgc cagcggaccg cccggcagac agtcgaaccg
    41341 cttggcagcc gccaccagcc ccgcactgac catcatggag acgacgatcc cgccgaccaa
    41401 tccttcccag gtttttttcg gcgagacccg cggaaacagt ttgtggcgcc ccatcgtcac
    41461 cccaaccgca aacgcgccaa cgtcactgaa cttcaccact gccgccagat aggaggccag
    41521 aaagacacct gcgcgggcag agggaatctc gaacatctcg tccgctcccc actgggccag
    41581 ccggatgaag aaactcaaca tgaacggcaa ataaaagaac cccaacagcg tgactcccac
    41641 atgctcgacc ggcttggcga tccgcgagtc aaaaagcacg cgcaacaaca gggcggcgac
    41701 cagcatggcc agcatcaggg tttcgaactg gtggccggtc ggcagtcgtc ccgccgcata
    41761 cggcgggaag gcgtagcagg ccgtcaacca caccactccc atgaccatgc cgccgttccg
    41821 gctcaccgga tagccgttgc gcttcatcat ctggtaaaac tcgttctggc aggcgctcga
    41881 catcaccagc agcaccccga acagcatcca tcccggcatg gcgcagaggc tcaacagcca
    41941 agcaacgccc accagcacac cggcgaggat tcggcgcatc aacatggcgc acctccccct
    42001 gcccctgccg gttgcgccgt cactccgcca aagcgccgct cgcgtttggc ataggcggcc
    42061 agcgccgctt gaaactccgc ctcgcggaaa tcgggccaca gcaccggtgt gacgaaaaac
    42121 tcgctgtagg cactctgcca cagcagaaaa ttgctgatgc gccactctcc gctggtacgg
    42181 atgatcagat cggggtccgg cacatccggc gcgtcgagcc gggccgccac agcggcctca
    42241 tcgacctgtt ccggcgtaag cgttcctgcc cggacctcct ccgcgatccg ccgcgccgcg
    42301 cgcgccagct cgttgcggcc gctgtaactc aaggcgatga gaagctggcg ttcaaagtgc
    42361 gccgtttcgc gttccagacg tgtcagggcc tcccgcaacg gagccgagag atcttcccgc
    42421 tgtccgatca cccgcagccg cacctgacgc gcgatcagtt cccgctcgtg cttgtggatg
    42481 aaggttccca agagcgccat cagccccgaa acctcatccg gcgggcgcga ccagttctcg
    42541 gtgctgaacg catagagcgt caggtaacgg atcccggcgt cgcggcagta attaagcaca
    42601 caacgaaccg tttcggagcc ggcctcatgt cccgccagac gcggcttgcc acgttgtcgg
    42661 gcccagcgac cgttgccatc catgatgatg gcgatgtgcg acggcactct gttggtttgc
    42721 gtgtccacac tcattccgtg tgcgcggtac tgcggcctgc ggtcacaacc ccagccgcaa
    42781 cgtggcttcg cgcgcgggtc ccactgaaag gattcccagt ctcccgccga tcaacgcgat
    42841 caggcgctcc acatacgcgc gggccttgag gggcaaatcc tcatagcggg tacagcaact
    42901 ggttggagtg agccagccgg gcatctcctc ataaacaggc gtgcagcgtt ccaagacgct
    42961 caggtcggaa gggaagattt cataccggac cccgtcgtcg cgctcatagg cagtacagat
    43021 ccggatcacc ggctcctcgt caagcacatc cagcttggtc atggcccaga agtccacacc
    43081 gttgaccatc gcggcgtaac ggcccactac ggcatcaaac cagccgcacc gccgcagacg
    43141 tccggtggtc gcaccgaatt cgcctccctt ctgccgcagc cgctcgccgg tttcgccggt
    43201 cagttcggtg gggaacggtc cggcccccac gcgggtcgtg taacatttga tcacgccgac
    43261 cacccggtcg atacggcgcg gcgagatacc ggaacccgtg caggctccgc cagccgtggg
    43321 gctggacgag gtgacaaagg gataggtccc gaaatcgatg tccagcatcg tgccttgggc
    43381 tccttccagc agaatgcttt tgcccgcgcg gtccgcttca ttcacgagcg tcacggtgtc
    43441 ggcaatgtac ggggtcaacg tccggaccgc ctcttcatac cgtacccggg tctcacgccc
    43501 atccagcagc ggcgcgcccc atccggccag caccgtgttg gcttccgcca cccccgcctc
    43561 caccatttcc aggaacgaag gggaaagcat gtcccccgcg cgcaagcctg atcgccctat
    43621 cttggcaccg taggccggac cgatgccgcg accggtagtt ccgattttcc tctcctgcga
    43681 gcgccgtgct tcatcggccg catccagcgc acggtgccaa ttcatcacca tgtgggcccg
    43741 gtcactgata tacagccggc cgagtggatc aaacccctgt tcccgcaggc cctcgatctc
    43801 ccggatcagg tcgaaaggat ccaacaccac gccgttaccg atcacgcatt gcttgtccgt
    43861 gtgcagaatc ccggacggaa tcaggtgcag cacatatttc cggccatccg cgatcacggt
    43921 atgtccggca ttgcttcccc cctgataccg cacaatcagg tccgcctgac tggtgaggac
    43981 atcaatcacc ttacctttgc cctcatcccc ccactgtgca cccaccaaaa ccgtattcgg
    44041 cataactgct cccgcatgag aacccgtacc ggcccctccg gcacaacgaa gttttactat
    44101 acccgattac gggagccggg ggcaacccct gttgccggaa agccgaattt ttgacttggg
    44161 gaacgacggc attcaaacgg tgactgcgga tgagatcagc ggccattgtt caacagccgc
    44221 atggtccagt aggcccacag ccaacgggcc ttgcctgcgg catcctgagg ttgggaagtg
    44281 agatcgaagt ctttgaccag acggtcggcg aagcgctcct gaacatagcc gtcttcgttg
    44341 ttgagccgat cccaggtgac aggcaattct tcgggcctga ccctgcctgc ggcaagcagc
    44401 tccttcagct tcgcctttgc ttgatcgtta cggctgtact tcacgttgaa cgcaagtgca
    44461 agcggatgat cgcacgcccc gagtctcgaa tagaaccaac ggatctgcca agggtctcgg
    44521 gcgatccgcc agtggcagtc caccaccgcg agcagttcgc cgaagagcgg ttgtccctcc
    44581 gtcaccgtgg cgttgagacg ctgggtgtcc ttcagcacca tgaagagttc atccgccttg
    44641 agcatcgtct cgcgttcggc ggttacgcgg tattccatcg aaagcgccgc caagtaggga
    44701 atacgcacct cgtcggtcat gttcgaccag cacgtcttct cgatttcagt tttgtattca
    44761 cctgaacgtc cacggtaagg gcatgcgtcc cgtgcgcccg catccgccgc cttctcgaaa
    44821 cgaaacgagt agacgtccgc atcggaaagc ccgaattcga tccgcaccgg cctgccggcg
    44881 aacgccgaca cctcgccgtc ctcgaagtcg acgatacggt cgactttgtc gccgaagatc
    44941 tcgtcggtca caatcgactt gcccgcctcg tcggtgattt tcacgaaaac acgtccccgt
    45001 gccgaggtct tgaagttgag cagcatgcgg ttgccatcga aaacaacgcg cttggtgacg
    45061 agtttgtcgg tgcggtagcg tccatgatac gacacgaaac cgtccatccg cagcgcatag
    45121 cggttaattg tgagacggct gcccgtccac ccgccgtcga cttcgtagaa ggaaagttcg
    45181 tcgtcgctgc cgtaacgtcc gggacttacc acgaagcccc gctgcagata gccgtcgcca
    45241 tacatccaat tgccggcatg ttccggaccc ggcgagaaga acgcgtcctc ttcacgtgtg
    45301 aaggtcttgc cgtcccgcga ccacatgaaa acggtatcgg tgatcgcaat gccgtgccgc
    45361 ggatcgggcg cgaggcgttc gacgcgcttg tttctgccgc agagccgctc gaagttcttc
    45421 gaccagcgtc ggcggtcggt atagcgcgtc ggaaaaccga cggtcaactc aggattgcgc
    45481 ggatagaggt ccacccccga agtgtagagt tcgtactcct cctgtccgtc gttgaaatcg
    45541 atgttctcgg gcttggacca gttatggaaa tcctttgaac gggtcaccat cacccagcgc
    45601 gcgttcgtcc cgccgtaacg ttcgtattcg agcggcttca cgttgcgcat gtacatgcgg
    45661 tattcccggt tgacctcgtc gtagaacgca acgttgaggg aatcgaagcg gagtcccatg
    45721 tcgccgaact tgaagagcgg ccagcccggc ttccagtcga gaccatccgc cgagacaaag
    45781 caccacagtg ccttgccgta tttcgccagc ggcgcgtagt cgggatgcgc ctttgcgaat
    45841 tcctcgctcc acttgtggct gtagagcgcg atcgccttgt agcgctcggc ctcgggacaa
    45901 gccggattat cgtccctgaa cgccatgaaa ttgtcccagt agttgactct gaaatcgatg
    45961 acgatgttgt tctcgcgatt gcccttgtac gggaagcggt tgatcttcgc gcgctcccag
    46021 gtgagcccgc ggtccttcga ttcgaagagg cagatcttga gcccgtgata gtgcggctcc
    46081 gactcgtgac tgctcatccc ctgccggtac atccggtaga cgtcatcgac cttcacgacc
    46141 gacatgtagc tgccggaggc accctcccaa ggcttgtcgg tcgtgatgac cggacccacg
    46201 tattcgggat ggtgcggcac acgccgggca ctcgtcatcg ccggatcgac gatccagtcg
    46261 tcccaaaaga cctgcaactt gtcgcggatc gtacgcggag gcgtatcatc gtacgcggct
    46321 tcacgcgcga gtttaagtgg caccgcgctt tcgatctttg cgccgacgtc cttcaccacg
    46381 ctcacctgct gcggcatgaa gggatcaaca cccttcacga ccaatgcgga gtctttgccg
    46441 agttccacca gcttcaccgc cgccaacgca agtgcattcg tcgcctcgac aacgaacgtc
    46501 gcgccgtccg ccaccttgat ttcgtcgatg ttccgaaacg ctccgctttt accgagtcgc
    46561 accgtaccgc gccgaacctc aatccgacct cgcaccgaat tgacacgctc gtcaaaactc
    46621 tgggtgaaat cgcccgtagg atgccaaacg agattcaatt ccccgtcgat ccgcgcaaaa
    46681 cacacggcat cagcggtacc acgcataagg agcgtgccgc ccgtggatac gacatggtcg
    46741 gaattcttcg tgcccggcga actcgtcgaa tggagccgat cgatcgtctg gtcgaaaccg
    46801 ccgagatcaa aggtcgcgtt acggtcgagg gtgccgcggt agtagtctcc ccaggtgagc
    46861 actccttccg gcgccagcac gttcgccgct gcgcattcga ccttcgcgta cgcgagctcg
    46921 aatttgtgcc agcggtttcc ggatgctttc agcaccagct tcgattcgcc gtagtcttgt
    46981 ccccacagga cggtatcaaa ttcgatcggc gcttcgaaga ccgcctggtg tgtacacgag
    47041 cccggagacc cgagcgcccc gtagagcgtc gcccccgcgc ccctgatcgg cgcggtggcg
    47101 acaaaatcgg ccttcttacc gacgagtttg agcacatcgc cgacggcaag aacggtttcc
    47161 ttctcaatct gcggtgtctc cgccgcatcg atcacggttt caaccgcgcc caagtgcact
    47221 acggcgaaaa ccaaggtgcc aagaagggtc aactttttcc gagaaagggt aatcgtcaag
    47281 gttcctcact ttcctcattt gaaatgcagt accgttcctt gcatgagatt caagtacaga
    47341 ccgtcggcgc ccttgcgcag aaccgccact ttatgctggt tcttgccgct ctcgtccgtg
    47401 aaaacggcgt cgagttgcaa aacgtcgaca cggaaattct cgttcgtgcc gctgaacttg
    47461 accaactgat gcttcgtctt gctcccggca tattccgtga ggtccacctc gacacgctgc
    47521 ccgtcgcaaa tcgacaggga tttctgcgca tccagatagc cgaagccgtt tgcaccggca
    47581 acgaagcgca ggaccgactg gacgccgttg gacatcacca catgttcggc ctgcacgacg
    47641 ccgttcgaac cgatctcgat catccccgta ccctccgcgc caacgacgat cttcgccgcg
    47701 ccgcca
//
LOCUS       DAOSLE010000004        46491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_921_length_46491_cov_7.599944, whole genome shotgun sequence.
ACCESSION   DAOSLE010000004 DAOSLE010000000
VERSION     DAOSLE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 46491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46491
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_921_length_46491_cov_7.599944"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(636..4844)
                     /locus_tag="PK576_00545"
     CDS             complement(636..4844)
                     /locus_tag="PK576_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60111.1"
                     /translation="MKQLITVITTAALAAAVSASDGYWFVNQMTYAPDSLKWSNTKYW
                     LDGVPAQNGGVFTHASASDSMSQWVSAGVYQDVAGLEISGMAFPYSGAFLLAGNQPIT
                     FVGDAPYLRPKDEEGKYVDQKIPFLGNNNAFEKTGRGPLRVYDGAFYSGFTNVTFKHG
                     LVAVYAKSGTALTDGRVTFEGNEIRFEPVAAEGSAAALTIATNSAVALKGSVRFSLKK
                     GSNASASLTIGEVVREDVFPGTLYLAPNGGTGTLGTSEKFFLTNPAALVHAGSGMVSP
                     VIALGGPNTVSTPVCFARYDASNGLVPATDLYHDGFAGADSNAIVRITANTTLSADTA
                     IHALVIDGNAVLTLNPGVTLTIGDGTSPAGIILQGFTTTKTCISGGAIDFRGSEGIVW
                     VQDNNSKTLQTIDTEIRGTAGVTFTGRNNVDYVNTILSGTYANSGDIRAYWGRVQLYA
                     DLTSAPAVRVFGDRLCGAQFLNYTKTAISELSLAGFGISTYHGRAVFTGTGEDFSTNI
                     ARVVLADDAGIRMNKNLQFDCPVTGPGGLVILQDSNKLTLNSTNTYLGVTRLETGTLT
                     LGANGTFGAGEVINNTTITVNKNADFVISNAVSGVGSWINSGTNTLLFASPSVTMGGL
                     TTQGDIGIAGAATRLGNLAQSSERAITPIAEGADAPAMAEIGTSSDILYGGYLRDNGT
                     NLIGLTKIGSGTLGIAKPQSYSGPTVVREGTLKLYRELIPCEVDIAYHLDANDAASVQ
                     TDETTGAVTGWVDRVTGVAFKQTWDGLPCPVYSNGVGGAIQSKPAIYFNGWTNCLVAN
                     KAFATKTVLILFQTTGYSVAGGGMDCLWANTRGDHGSRFLRLASATSIDTGTEGSVFH
                     YSTLANLSINGVAGVNTFTPNEPTLLSAFRTDDFGAGNNVIGNNYQGRSLCGFIGEVI
                     AFSRILSRDEIKAVENYMWQKWMGTELHAEAPAPVHGTLPAGTTLSVEPGAAFDLNGI
                     DQTVSTLSGIGGNIINSGAETATLTVTGTNGFRGTIGEKVNLALCGAADMALRVEAGT
                     VSFGGASAVTVFNDLPPQDGLQYWLDATDAATVRRSADGYVTNWVSKAGTEGLAFGLE
                     KQAWDAETSQPPTYTGSINGQSAVFFGNATTNWMRSNKSIAVQTLVMVFQAFQNQMNM
                     AGYWGVNSGGDLGVRGSSTNFSYGTVGNCFVGAEAWHNGIALPASASGFDYALNTPVT
                     AHIESPVKAGPGNNAIGGFYVNSPTDTRRFRGAIGEVIAYNRMLTKAERDQLEHYLAR
                     KWRTPGYTVNTEVFAAGSEVTLTAGASLNLRGAPLVVARLTGGGEVTGDVTLTDTYTV
                     TLDETGGCPNPLRVTGDLTFTGCRLVVVGTLKNALHKVKIAEADGVLTGLPSWDEPQP
                     GWQVVREGGAVYLYKELGTTVILR"
     gene            5302..7509
                     /locus_tag="PK576_00550"
     CDS             5302..7509
                     /locus_tag="PK576_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60112.1"
                     /translation="MRFAMMGMVLGLVSGVWAEVTIHVAPNAPAGGDGRRRSPYATLE
                     QARDHIRAARKEGLIARDEPVTVLLEPGVHTLTQSFELTREDGGSEAAPVVYRAAKRG
                     RARLRGGLTLPADAFQPVTNGAVLNRLDPAVREHIRVCDLTSRVPGGFEPFKNAYSGT
                     PAAPWLYHNRKPLTLARWPNADAPQGGWAGFTKAVETGKPIPDSPDPALRKPRPGSFE
                     FADPRPARWNLEEGVWLMGYWTHDWSGEVIRIASYDPDKKVIALAAPHGYGIAAGTWG
                     SAVRRFFALNLMEELDAPGEWYLDRTSKQLYVYPEGETEEGGEWVLATLTQPMLAVSD
                     AAHIRVLDLAFEYSHCAGVVIILKNSEQVEVAGCVVANHAGSGISVSGRANTVRSCDL
                     YQLGKAGLSLNGGDRKKLIAAKNLAVNNHIHHYGLFQRTYAAGIALGGCGQIARNNCI
                     HDAPHNAVLYGGNEHLLELNEVYRVVMETGDAGAFYTGRDWTSQGNVLRHNFVHTLGR
                     GDAKHVNTMGFYLDDCDCGDRIEGNVFWRAGRAIMIGGGRDNPVINNLIVECPIGLHI
                     DSRGMTWKYWNNTNDPNWRLDAKAQEFDYRNPPWSVRYPKLASIMDECPREPLNNPAR
                     RNVFVDCVDTCSFGGKVMDLLHKMELEDNLIVNTVGTNRVGKLREGINKGFRTVSGTP
                     DKPFDPGFEDAPNGNFALRRNARLFTELPAFERIPFERIGLYTDTFRRKLPRR"
     gene            complement(7590..8936)
                     /gene="miaB"
                     /locus_tag="PK576_00555"
     CDS             complement(7590..8936)
                     /gene="miaB"
                     /locus_tag="PK576_00555"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOE60113.1"
                     /translation="MRFLLHTYGCQMNVRDSDAAAAVLKAAGHTLVDREADADLILVN
                     TCSVRGKAEDKALGKLGLLCAGKREQPERIVGVMGCMAQRLGAEIFTRVRGLDFAVGT
                     RRAGAIPRLVARVLAGETGLGEWSAPDELPDVPDSHAESGFSALVTILLGCNRRCAYC
                     IVPDVRGTEYSRPAREILAEIDALTRNGVREITLLGQSVMNYGRVHTVWNAADAPSPG
                     GFREPFARLLEAVAAVPGIARVRFTSSHPSGVTDELIRVMRHVPQLCHHLHLPVQSGS
                     DRILKAMRRGYTRAGYLDAVARLRSAMPDLALTTDVIVGFPGETVADFEETRSLMREA
                     AFDNAFIFKYSPRPGTPAAALEDDVAEEEKMRRNQVLLADQDLRGVALNRQLVGTIQE
                     VLAEGPSLRNTARWSGRTPGNKIVIFDPPADLAAGDAVRVRITRAAPQTLYGDPLP"
     gene            complement(8940..10835)
                     /locus_tag="PK576_00560"
     CDS             complement(8940..10835)
                     /locus_tag="PK576_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOE60114.1"
                     /translation="MWRLLSILGRVCRILFWLALILVLAGILVLYLIGHDIPAPLVQR
                     LAQAVSTDDVHVRIGRATFTLDKGLRLLHVKALPKRIADGPLLAVEEVAVALSLLPQT
                     PMQARIRGVTLKNLTFPDLPPPMSDPPHSLPELPDLAPFPLRIEQADVLGLRVRSLVA
                     NVDVDTNRVAVSEALIHWPDNVFGMTVTGQATLDLTRRHVSGLVAGKAFPDNLMPLFE
                     MLHARGAIRQINCFSKIARPVEAVYAFDVDIDSTDFAMRLGLDVGPCAYRGVPVSYAK
                     GTLFLYGTNIHTTVVIDPLEAQTADGAPITGSLAYREETEGLEIDARTVMHLPPFTTI
                     INVLNRGELDRIRCDTPPSLSVRGAIALSSQESTITNDLRGRVALDKGTILNFGVRDL
                     TADFDMQGYSARFHQAAARSASGGQVNGEVTFTFPEYAATATVFSADIRFTGVELAEI
                     SQAFNVTNERVGLVNGNLHLTGPAYGDTVAGLNGSGHANIRNGVIHRMRLFAGLTDYL
                     TRNIPGISSLVTQSSSSMDFTLCNGVFATENLLLEGDLVSIRGRGTYDLNTDKLDFTV
                     RVSIFRQKTIAGRIVRLMTLPFSRLLLEFRVFGTLEQPEWTYVNLIERITEGFSDAPG
                     TVSQPKP"
     gene            11369..11629
                     /locus_tag="PK576_00565"
     CDS             11369..11629
                     /locus_tag="PK576_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE60115.1"
                     /translation="MNVEFDPDKSAANLKKQGTGFLEAQALWEDADRLLVPARTQGGP
                     RHMLVGKTGQKHWSAIFTYRGEAVRIISVRRARKEEVDAYEN"
     gene            11619..11849
                     /locus_tag="PK576_00570"
     CDS             11619..11849
                     /locus_tag="PK576_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011743982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOE60116.1"
                     /translation="MKTEVLDKAFDNGDDVSKYLNFSKATRPGRELKRVNVDFPMWMV
                     QSLDKEAQRLGVPRQAVIKVWIAERLRVRTHA"
     gene            12103..13299
                     /locus_tag="PK576_00575"
     CDS             12103..13299
                     /locus_tag="PK576_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009669088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HOE60117.1"
                     /translation="MKKNSLNQGLTAAKKAKQDEFYTRYVDIQKEVEAYLEFDPDAFR
                     DKVIYCNCDDPFESNFFKYFAAKFNTLGLKRLISTSYDGSPIAGHLTLFPEHNEGAGK
                     RKKPDALAVIIDHVKDEDGGGAANVADVELFLKRNKAARIALKGDGMYPGGDFRSAEC
                     VAFLKEADIVVTNPPFSLFREHVAQLVEHGKKFLIIGNTNSITYKDIFPLIKNNGMWL
                     GCTNFNVGMFFEVPDHWGHFHHIDQETGKKIARVSSSCWYTNMDHGRRHQPLSLMTMA
                     ENLKFSRNLRGKTTYDRYDNYDAIEVAAYKEIPSDYDGVMGVPITFLDKYNPDQFEIV
                     GTTESNDPNNACRTRVYTSQECRDAYMAIFGKPGVYDLNASGVVNGVKVYKRILIRHR
                     ALPHNS"
     gene            complement(13727..13846)
                     /locus_tag="PK576_00580"
     CDS             complement(13727..13846)
                     /locus_tag="PK576_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE60118.1"
                     /translation="MHRTYVGMIEHGEKNITLRNIAKFVKALRTPIHELLNLE"
     gene            14182..16305
                     /locus_tag="PK576_00585"
     CDS             14182..16305
                     /locus_tag="PK576_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60119.1"
                     /translation="MRYSKAVWAITAVVAVARSVFGVNWKSEVTSGAWDNAANWEGGV
                     VPDAVTPASIENSGVDYSITLNAAGTTCATNTVIGNAEGSTVFTIHSPLSFFDFTSSS
                     PKYYYKQNFGAKTIIGAGGAFNFLASTSDGLSSDQGLFITKSEFAVDGGKVDLRMNGH
                     VALSGTAQQPATLTITNGGSFAWHSSDGYGQFKVPENAKVRVHDGSLAFSQNRNHYHT
                     APFSMTGGEMVFSGTSVFSNSYVSAEGYTSVALEIRSGKMEFHDEAVCVVQSKAGVHS
                     DFLLIPSDAGKRVELTVTDQACFGVGVEPYLMTVGEWTPGGIAVAEFSSTEQCNLGRT
                     TAVGAYAGYGELNLRSGLIVLGKTYGLMVGTTDNQALNRARGDNAAHQPADAATWAPT
                     GLVRVAGANLYLSTPFVDTGYGCKGLAGFRIGDGVYARYNNKAVPAPAVGTLELSSGS
                     ITNAQGYWSIGAGDGYGRFVQTGGTLLKCDPDYPTYGGNQTMVVGFVGGRGEWVMSNG
                     TARVIAPMMIGGAAPDDFLFADINTGWCPFSSHDAVGTLTIACADKEKPCSLEVIKAN
                     TGNTDVQGVWLGRDGTGTLEMIGSGGSLKTPYLILTNGYENASGELLTHGKAKLRFVL
                     DEDGIASLTVSTKMAIADNAEIEVDGRAYTGETKKVKLVNVTGSRSGSFRPENIRVKG
                     GVLIQDEDRHLYFRHMKTGLSVMLR"
     gene            16321..19344
                     /locus_tag="PK576_00590"
     CDS             16321..19344
                     /locus_tag="PK576_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOE60120.1"
                     /translation="MKYCAMMAVMAAVGSALAGGNLLTNGDFEGGRLTGWGNCASPGG
                     STMSVTRDAHAGGYALEYTHNGIGNQAFCRNFVKVVPGAVYTIRGYLKGDAEKINIRL
                     FNYAEGMSYVGETDIYTATKLEDWTYFEKAFSAPEDIKSVGVGLLLVKGRAYLDDVEV
                     LEGNQIAEEPNVLWNSSFKLSYHDNGMPEFWNTGGGHSFQTFQQGTHEGKFFRMADDV
                     ASPVEGARVLYVNKVGRTYLRPGPLAGGTDYTFSVYARAVTGEAKMNLILRGMRPGKK
                     AFTVGSEWQRLIFTAHLDRAVAEATATLEYNRNELYLAAPMLHAGKTALPWKPSPVDA
                     TFPDLFAGNQVPPPPAKIETIALPRGGDWSAAVKIDRFYVHGDYGKHVDGRYQAAAYR
                     DGDVLKVAFEMASGKKPPVKSKPHGPLVTGNEAVELFVSPQANGAGALHFMGGRNGAY
                     ACQDNGNGQAEINEGWAFAASETAQGWRGIFEIPLSLFTQPGGEVWRMNFAVTEFDGV
                     KPLYFSAIRAGFHSAPDFFRVTGLPRGSKVEPPAVFVEYDFYTTEKTARFRLRNAKAP
                     VEMEVTGASVPLAQDGHVDHVTLTLSGNEGEIEIGAFADGYYEVVVKAANGEGRSHFR
                     KFADEKHYTRINRFNETINHDGQPFFPIIFTAWMGGGRNDKIPTDWHIGELKKRNFNT
                     VMIPNELYVLWDTRNSSEMRRTIAKKFTDAGFKLLIWSACGNLKEEGMMKAVVDYRKR
                     HIDELAEFKKDILGWYYLDEIGANWEKSFGVKLEDYPKGYRAIKDYDPARLHFINWNH
                     TGCTVGTKYFAEDGATDIYSLDCYTYSCKPNFGSLSAFEIGTSTLRSRMDDTHLPGIE
                     WLQTYAYQEGIREPHQLEYRNNVYVALVNNVTGYMHFIDVPEHKELWEGMGEAYGTAL
                     KWLGMTVEPGSRLVRRGRQGQISYALWERGSGGHAFIAANTLYAPQTEAIVFTVELGS
                     SVETVGGEGALESNGRSLSVALPTAGSAAWFITGR"
     gene            19496..22210
                     /locus_tag="PK576_00595"
     CDS             19496..22210
                     /locus_tag="PK576_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60121.1"
                     /translation="MGRQNVIRNVRGITLVSCGLALMEMAFGAPVLTGDTSKPAASIF
                     YPKDRVSLYFHVSGLAAFQKDITLKLTVENEDGKVFWGRDCPVKPDANGQDEVIVTDA
                     PGGRLGFYRLRARLSDGTLLTTPWATRQPGQLTWAVVPAPEEREPVDVSYLRFFNSGD
                     PDLVGRMAVDRVGPTNPKIGPFGITSQWRHLEAHRPGEYADDPEADRIPLPYAVGNLY
                     THSTAWGNDLLATHFKMQGHADGHPPALTPAGEKAWEAFVKTFARNFRRQYPTVPRVY
                     EFTAEYYAPDNGSRTLKDIVRMYEVGYKALKSEDPEGLVAGPGFGYAAKDYHQLEFLK
                     AGLARWLDVITEHSYYTYPAEPNGIVDAIRTSRQLVRQYAGRDLAFYSTEFGYATEDF
                     EPRERDQLRHILRVSLILFGEGFKALSAFVPSDYRLEPGFGYTYNLALIKDVDRKDDW
                     FHNKTSPKPVYPAYAALTHLLVNSETLGDVPFLGETAWGYVFRKANTGRIILALWDWS
                     GTPRAVKLPAGVPVVDVVDCMGNSRTVKTVDGELSLTLTDVPQYVTGVSPDVWTNPMG
                     ERAKLAAAFQARQKAARSARGVEITGVSSACMDDGRAAVDVTLANLRAETNEGVVRVR
                     VKGNPDGRASAPYRLSPEQTVSVKVPMAADFVGAALMNEEVEVAVEQKAGPRVECGEN
                     VNFLFAPYRPKVAVDGIFDDWKDVSISRFDPARQLTNGKRYYKGPDDCTATLSVCWDD
                     RFLKFLFDVVDDQYVPPPASKTVWSRDSVQMAFSGVYHLIKTGNMLQDKLNEAATECT
                     WGLTEKGPYAYRHVSFDNAICPRGPLDLSTHPFHASIALCANGKSRIRYEIAVPWRDM
                     RVAEPKVGVRVGWACTVNDWDPGKPAYSAFGAFNLKIPDQFGAIVLTP"
     gene            22217..23539
                     /locus_tag="PK576_00600"
     CDS             22217..23539
                     /locus_tag="PK576_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60122.1"
                     /translation="MMFATGLFVLAATLQFNGIMGQSAVPGDPPLTFTGVRALTEGPG
                     GRLLLTGDDGVLYALVDGEVVSLGVRVPAAHLAWDGRTLWVLGSDAVWTVDEECPGCF
                     AAHKAFDVPGLHFDQAAVRPELADHPFAGKGKFFRWDRGRNVLVAHDAAGRECGTVFA
                     FPRRKGDSALVALGFLPGTEDLVASSYYPDVRLYRFRVDGSQVTGNGWPANRGYGLLR
                     RFKGRLYHMGTDVLPLGENLLGARAMRVGHEAHHRGWATDGVRDYLATSQGLYVREPG
                     ETRFRTRRGGIRPLTALAVNAPYVFLSMGGTVRWLYLDEDATGVFASSDDDNLRVANA
                     WIYPPLDMVPEGANLLVAGGEAGLYCFHPFLPPGASPRDHWEKRAPGPCPRVPGCRET
                     FPGVEAAIVAAGAALPEGIEPGLVARQGKWLVVEDRKNFRLVRFKVLN"
     gene            23570..25345
                     /locus_tag="PK576_00605"
     CDS             23570..25345
                     /locus_tag="PK576_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60123.1"
                     /translation="MMGNKTLIAALLAGCGTVVFATSFPNADGSGDIASPTAWGGVLP
                     TDQAQFQTSDVTNTASANVTFAGVEVGAKNVVFDFSQTPARTITLSDHFRPLNYTGAS
                     YTLRGGTWNLSGKNFHLYHNWGQGSECTVTLENGCNVTNVGWTLLNFSTLKCHLIIRD
                     QASLTVNTYDSNSGHGNDEPCLEVLSGGRLSVPATGSTLRIGDGTTLVSGAGSRIESY
                     GGAGDFTLGNTYHNTLIRITDGGAFDAASRRMNLNLNDATNTTIVVDDDGTFQCGEVY
                     PNGRAFNIQIGERGQMTTGRWYIKGNDHSVRVLNGTLKGSRWIGEAASGASVTNFSVA
                     VIGSDAQVDIAGGDALFMGLSDSSILLEGASWSCPVTYWKSRESSNCTFRLTQQAAFT
                     TTGHFWGTAYDGGSASQTNCNGNVISVESGSTFQSAGFTFVGKGNRLIVTNATFRCPT
                     GSFNFGRSDRGSDANQLVLQGTTPRVEIASDAMHLRYGSTLRVEMPAEGYAADFVPIT
                     TAWSLSLDDSCHLVADVEAIRRHQRVNKTYVLARANGGVSIPESVLAEANAVAPDEWQ
                     FGVSEDNKSLTLRVMLLRGMAVMLK"
     gene            complement(25438..26418)
                     /locus_tag="PK576_00610"
     CDS             complement(25438..26418)
                     /locus_tag="PK576_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOE60124.1"
                     /translation="MIPELQIFLILCCFAAVAFFSATEAGVLSISHARLIHLVRAGMK
                     SAVLLNGYLADMQRFMGAVLVGTNLTNVILSTLSASLAQTCLPNSRTLQSVWAVTMAC
                     ILLFFCEYLPKLFFTTRPLRRTLMIIRLFYFVEKAISPLTALVLWLTKWLTPQKAGAQ
                     GQRFLMTREYIQHVVSDARNGARISAFERLMINRVLTLQSMKAEQVMTPLARVTKTVE
                     DAPLEECYRLVRESGHVRLPVFSADGSRCVGVLNVLSLLAQAPDPKRTLARDGMHPPY
                     FVRADECADNVLPLMRAYRQPMVLVRATQDNAVLGIITEENVLSALTGSL"
     gene            complement(26415..27734)
                     /locus_tag="PK576_00615"
     CDS             complement(26415..27734)
                     /locus_tag="PK576_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE60125.1"
                     /translation="MNPIFFMLNGIVLLTLFALSAFFSGSETVLFSLSPIHVQRIRNR
                     NAEAAARLERLLANPSLVLSTLLVGNTLVNFAIATIGYLMFSRLTPQWGEVIAIPVVT
                     IALLLFGEVTPKRIAVLHAERLAPLCSRLLLFWLWLLRPFNLVLTAASRVFKHALRRE
                     RRALSNNELLTVVEVGEEQGVIDAEEASMVDGILRLSELMASDEMTPRVDMVGLDLDT
                     PVAQQVETVRKAQFRYLPVYVRTPDAIEGFLDATMFLLDPTHNARKATTPALFVPENV
                     TLDDLLVTFQRSGKHIACVLDEYGGTAGLITRGDILELITVPVARNGEERTPAIRNLQ
                     GDIWLVDGTVSLDELNHRLDLQLEADDADRIAGWITFHAKRLPQPGQVVEAQGCRVTV
                     RRLRKRRIDVVQLEVLERPEETPEDLQEDLQEVDDLPYDHPEEGELR"
     gene            complement(27731..28504)
                     /locus_tag="PK576_00620"
     CDS             complement(27731..28504)
                     /locus_tag="PK576_00620"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOE60126.1"
                     /translation="MKSTPGCVCLLCVILIGAVCRADDESRPATAAAALEENLTAAQI
                     LSACTQLIPTDRLLLQGTLTVRKLRGIKLAEEPFKLMLDWGARPPSAEVLLLDATGTS
                     LVERAVLTRPAGRPAQISLFRGPEQQPVDSPSYAGRVRGTDMTWMDLTLDFLWWTDVR
                     FDDVPRGQSRNGRDCDILVTVPPAPIPGCSAVRVWVDRQLRCLMQAEQLDPQGTPVRK
                     MWVQRVKKMDDRWMVRDMEIETLNSGHRTRLSVIDVSSP"
     gene            28696..29487
                     /gene="lgt"
                     /locus_tag="PK576_00625"
     CDS             28696..29487
                     /gene="lgt"
                     /locus_tag="PK576_00625"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOE60127.1"
                     /translation="MALGFLFGWRTAVLLCKRTGQDADQLTTLLTWLMISALLGARAA
                     YVLEHWHAEFADAPLMILRLDQGGLMFYGGFLAAALVLLLYARIHRQHVLSITDLVLT
                     VVPLGHAFGRVGCFLHGCCYGRVTTSRFGVAFPQGSPAWWEHVSAKPPLISQHATASL
                     PVIPTQLIEAGANALLFVVLFTLYPRCRTLRGLLTGCYLMAYATLRFGIEMLRGDPRL
                     AVGPFSISQAISLVLFALGLALAVYSPRMRLDVRIAQNTLPHMEQ"
     gene            complement(29448..30704)
                     /gene="thiC"
                     /locus_tag="PK576_00630"
                     /pseudo
     CDS             complement(29448..30704)
                     /gene="thiC"
                     /locus_tag="PK576_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010902492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
     gene            complement(30721..31590)
                     /gene="thiD"
                     /locus_tag="PK576_00635"
     CDS             complement(30721..31590)
                     /gene="thiD"
                     /locus_tag="PK576_00635"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOE60128.1"
                     /translation="MSDERNDECACLEPVRLPVALTIAGSDSGGNAGIQADLRAFHVF
                     GVHGCTVIAALTAQNPFGVRAVLTADAAFVAEQLDTVLDVYGITALKTGMLACPEVIE
                     AVADCLVCHNRIAKVIDPVMVATSGARLLAEDALEMLTSRLLPLATLLTPNLAETEVL
                     LGSDRTVQTLEAMQEAARELADRFGCAVLVKGGHADSPCAEDVLYDGAALHRVTTPRL
                     VSPASTHGTGCSLSAAIAASLALGRPLVEAVTEGKAYVYESIRTGAQVGEVATVLGTP
                     TRLPCDRVTVETV"
     gene            complement(31587..32546)
                     /locus_tag="PK576_00640"
     CDS             complement(31587..32546)
                     /locus_tag="PK576_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOE60129.1"
                     /translation="MGFAKQVGMWLAGISLASAVTGAEPTSATSQRFVRYRSGFNRDV
                     LSLQVLLDRRNLSCNCVDGMWGKRTQIALMTWQTLNGLPATGVPDAAVLEALGGDADV
                     LTRHTVTEADRAAIGPVPESWEERARLPSMTYETIQEKVAEQGHTSQRALARLNPEVA
                     WPNPPVGTEIVLPDCTTRVPSVKAGSVRIALSRMEVTVFDAAGKLIALFPCSIAREKR
                     HRPDGELTVKNIAPNPNYLYDPHLFDPKGPKRSKLVIPPGPNNPVGLAWVGLSLQGYG
                     IHGTPFPEQIGRAESKGCFRLANWNAVRFLNLVEPGLPVVVEE"
     gene            complement(32804..33583)
                     /locus_tag="PK576_00645"
     CDS             complement(32804..33583)
                     /locus_tag="PK576_00645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE60130.1"
                     /translation="MITETYYDTHAHFTLTAAETVAVLSRAREAGVSRVVAVGGNAKL
                     NAGALAAAQAFPDAIRLALGFDRDQATEAEPEVFVETLRQLAETYPFAAVGETGLDFH
                     FHPETAEAQCALFAAQLRLADVWKRPVIVHTREADDATLRVLDETPWHGQGLRGVIHC
                     FTGDTRFAAQLLDRQMALSFSGIVTFRQAESVRASAAFVPDDRLLIETDSPYLAPVPM
                     RGQRNEPAYVTHVAACLAAVRKTRPERVAEITTRNARALFG"
     gene            complement(33628..34089)
                     /locus_tag="PK576_00650"
     CDS             complement(33628..34089)
                     /locus_tag="PK576_00650"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOE60131.1"
                     /translation="MSALSKLLQRTLNLRRKETERKELPRSARVGAWGEEQAAAYLSA
                     RGYTVLGRNVRPNRRDELDLVVRQGDTLVFVEVKTRHRETFARPARAVNPAKRHALNR
                     AAVAYLRKARFPDLYYRFDVIEVLGQPEEGEPLIRHLENAFSFERRFVFPV"
     gene            complement(34094..34681)
                     /locus_tag="PK576_00655"
     CDS             complement(34094..34681)
                     /locus_tag="PK576_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOE60132.1"
                     /translation="MSENVTNVSLVGVGGQGILLTSDILAKTAVLAGLDVKKSEIHGM
                     AQRGGSVISQVRFGEKVYSPIIPDGASNLLVAFERLEALRWRHLLAPDGKVLINSLDM
                     TPITVSSGQQAAVTDLDDRLRAEYPSALYVDALTIAKEMGNMRTMNMVIAGALSALLP
                     FEPEIWKQAMRERIPGALIELNLRAFDTGRQAISG"
     gene            complement(34674..35531)
                     /locus_tag="PK576_00660"
     CDS             complement(34674..35531)
                     /locus_tag="PK576_00660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE60133.1"
                     /translation="MGKKKTFKPNDGRCFVPKSKRKPRDIKNKLFEGDCLEILPFFPD
                     ACVDLVLCDLPYGTTQNKWDSLIPLEPLWEQYRRILKPRGVVVLTAQGIFTARVILSN
                     EAWFKYKIVWEKSKPTNFLNAKRQPLRKHEDVCVFYARQPVYNPQMNSGEPYDKGVRK
                     NQLSGSYGDFQPARVKSSGARYPTDVVYFKTAESEGPVWHPTQKPVELGRYLIRTFTE
                     PRALVLDNAFGSGSFLVAAALEGRSYVGIERNQDVHLFKKVQVDYVGVARGRIERSSC
                     TPPEGEEHE"
     gene            complement(35549..37324)
                     /gene="iorA"
                     /locus_tag="PK576_00665"
     CDS             complement(35549..37324)
                     /gene="iorA"
                     /locus_tag="PK576_00665"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOE60134.1"
                     /translation="MSQRVLLSANEAVAWAACKAGCAVASAYPGTPSTEILENVAKFK
                     ASIYCEWAVNEKVGMEVAIGASISGARALTAMKHVGLNVAMDPLMTYTYVGANGGLVI
                     ITADDPGMHSSQNEQDNRTLVRFARAVLFEPADSQEAFDMVIEAFEVSERYQVPVFVR
                     LTTRTSHTSTVVDVGDDFAPGHPPRKPYVKDIRRYIPVPMFARPQRLKAQERTAVLRD
                     LACASPFNRIEAGDTRFGIITSGVTYQYVKEVFPEFAVLKLGFSNPFPAERVREFAAS
                     VERVLVIEELDPFIEEQVRALGIPVVGHTTELNMFEMNPLRLMTLRNELLGDITLPEV
                     RRADAGLPTRPPVLCAGCSHRGVFYTLAKLGATVTGDIGCYTLGAFPPLNAMDTTICM
                     GASVGNAYGMEKAGKGGRLCAVIGDSTFFHSGMTGILNVLYNGGRTTTVVLDNRITGM
                     TGHQDNPGSGKTLCGDPAPVTDIAEVAKAMGFRRVVKVDAYDLKTLEKVLAEELDADE
                     PSVVVAGGPCRIAAKLMNAGTFEVDAEKCKACGACFKLGCPAIERGETVVEGKRYKAR
                     IDPVQCSGCDLCPQVCKFDAIKRVR"
     gene            complement(37601..40855)
                     /locus_tag="PK576_00670"
     CDS             complement(37601..40855)
                     /locus_tag="PK576_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HOE60135.1"
                     /translation="MGMTTMKFVMGAALATFVSFSHAATTYYVNGTSGNDANDGTSAA
                     PFKTIGKALTVAIDNDRIEVAPATYKITASLLVDKAVEIVGTGAEASDTYVLSGRENS
                     SFSGRPLVVSNALAKVANIKFRGGYGRYSDGIYPANCAWIYAGTVTNCVFADSWRVNK
                     GYGGVYAQTEDAYVVDCEIYGHFNVDGAGSNQGGIFCAAGLTLHNGTARGCYIHDNVS
                     RQNTGGVLLSGANAKLLDSSVIGNVGCGYDRVNGLTPWPGSGAGGIMIRNANAVVSNC
                     VVSANISGERMTTGSDNHYRAGGIWFASAGKVYNTVVIGNTSASEGGGVWGSGYLYNC
                     VIADNVAQFRTGGGVYGKAFLYNCTVTGNDSLLDGGVDGVTGSDANNKATVENGIVWG
                     NGTLDSANVTLTATYTEDPKFLDAAAGDYRLDIGSPCIDTASVPSVMLPETDAAGRPR
                     VANGALDYGALEAAPNFRVVGDPETFGVPATPAFGEHPDTTVGAVNASVPSPYTDATG
                     LTNVVCTGYTYVAQDNANNVTTNEGADTAYAAAYARLGTLTWHLLAEYKQTVTNRGNG
                     AGLVTGSGFVTRGATFTATATPAAGYRFWRWEGAIGDNDPTSPTLTLVSDFARTVTAV
                     FSRDWHVDGVTGDDGNPGTSTAPFKTIHQALAVADDGDTVYVAPATYGFDPDHQALVI
                     TNAIALVGTGANRTDVKVSYKASTENWTGFGGKRLLYVDNKDAKVQHIDFAGPDNKYN
                     RSGNIDGSTAYINAGLLSDCVFRDAFVDIAAGCGVYLNTEDAVAEDSLFSGFGSVNGY
                     DKGGVALTIVNGLARRCEIINNNGRQNGPVGLSGAKARLFDSIITNNQSCAAHKHTET
                     TWGGPRSGGGVWIRNAGAVVSNCVIAANRANLPQGGSKTVCTGGGIVFAAAGSVYNTV
                     IRDNYAYLEGGGVYGVGNLYNCLIADNESEAGQGGAVSGAARLYNCTVVGNDSQLTDG
                     TDGISGDATLLNCIVWNNGAKNYDTAAMTYSCSSPKPTGEGNRDSDPQFRSSLSRGLY
                     CIASDSPCAKAGVWQDWMPGLKYLNGVPMPSEPGDVPMGCYAPYQIRGLIMEVR"
     gene            complement(41117..41914)
                     /locus_tag="PK576_00675"
     CDS             complement(41117..41914)
                     /locus_tag="PK576_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60136.1"
                     /translation="MKTRIRPFYFLHLASSFAFVLSANALTVTLSADPELPAPNILTN
                     GFFRFDEGLKGWANSHSNEVCCIEMEGRGLSFFTKDKKSVWRSIPTSVVKPGRTYILS
                     CEIKPSSKVNTHTFSNGGSGLGCGLTYWSEDWKRAVSLCAYTDGPDRWFRTYSQPVTM
                     PDWIIHGQMTVGLAYSGGSGYVDNIELVEAYSELTICAESQAAIRQVKVVDHNFRTVY
                     DSGVLNNGAKTWTHRLQIEAAYKYAVFAVDAVGNVSVVHYPAKKAQE"
     gene            complement(41924..44014)
                     /locus_tag="PK576_00680"
     CDS             complement(41924..44014)
                     /locus_tag="PK576_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60137.1"
                     /translation="MHKKTMTSGALALAMVLAGHAFAYETVMIPTLSPPNIVDGDLSD
                     PCWKKAYASAPFTAIKPGDKITEETRAYLFCTADTLYIGAKCSFTDYALREKIVNDKS
                     KSGFAGDCIELFLDPGDTGNYAHIAINVAGNVTSVGMVAPFRCSVQLYQTYWMMEAEI
                     PFAAIKLDAQSYNANWRINVARGNYKKGESSSWSKLENGVFGEPASFNSVNGIVADLV
                     AIMKAQKDAARKDFEIVFDRVVYTDQADVKATLDFLYTKSIAGFTTKATIVDATGKTV
                     LEHNAPLTAFHADFAFPLKGFADGKHVATIAIVDAAGKTIKSGEKTFYKVPPMRDSDN
                     VDRYTIRNHCVYRNGRFFFPIITWKLTSSDFASLEQWRSQSEAVLKEMAEHGFTTGMT
                     TDYSFPEEDLDVRRKTKSIAAWNQKITKSLHDAGVTFEDQCRMAKKYGINVIATSPYR
                     PKSPYATDCFVEHVARIRRTPNVMLWHTADETDGQVEYNTLLNRLYHELDPTRLTWLN
                     VINAVSANKDAADILSTDPYPIPNGRVTAVAAHADRLVANTKGRPQVGRWLWIQNFGG
                     EGGWTRPPTPAEVKCMSMLAINHGVKGLAFFTWAPLSQRNGVRQHPEGMSMFKAFSPF
                     LQKWTPALCQGEVKFQGRQGVLDVLAVEFEGRKVLSVVNIENNPVAAAPVEIPGFCDK
                     KMTLEGYGISVEEL"
     gene            complement(44168..44244)
                     /locus_tag="PK576_00685"
     tRNA            complement(44168..44244)
                     /locus_tag="PK576_00685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44208..44210),aa:Val,seq:gac)
     gene            44467..45693
                     /locus_tag="PK576_00690"
     CDS             44467..45693
                     /locus_tag="PK576_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOE60138.1"
                     /translation="MNRGLVVGMISLTLMGVRAETWVPEKMAESLHMGRRLEVFTNEV
                     RAAWGYAAPQKDAFLVLHPKQERKCAPLYVVLHSAGHDAFSCLACTRTPGNHDIYHAP
                     EEFFALYLDCRGNKGDWWWGIQRTKGPDIGPTEKRVIDTVKWVIEQYAVDPERVYLCG
                     NSMGGSGALGIGMRHGEIFAAIKANVPARVEHVSSRMGFPPYAPLPAGTRLADPPICI
                     DYSAPNDTWSTNHETFVSAMNLRKYPLYFYWGPFGHANNDGKILAVNDLVHSFDWLNV
                     RKNELYAVFTDASTNDPLPWPARLDDKKSGQINAFFRWKPLADRPRRVEVSLFLLTPQ
                     ICKTSFTLPTEATANVTLRRLQRFTVKPGAEVRWSFGEREGTVRADSEGLITIPGLKI
                     TGEPQTLRLVKPGWWR"
     gene            45797..>46491
                     /locus_tag="PK576_00695"
     CDS             45797..>46491
                     /locus_tag="PK576_00695"
                     /inference="COORDINATES: protein motif:HMM:NF037707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE60139.1"
                     /translation="MPPRLTKNKNKPPISAIPFEKCAAKTTTDGKPGTTVVEHCIHVG
                     RVAEALSALKPEYVKRLLPPRPGLPTGVHDVGKVSPGYALKYFANTVVREHAPELCNQ
                     SSFCKLHATISAAAIERWLNTPVTRHSPVALAAAAHHGKMETPPFPPDMCEILGGSAW
                     SEERQKLIARLSEEFDGTLQDAAEANEWLLAGLTCVADWIGSDETFFPADRLPIVDGD
                     AVLTARHAVKECG"
BASE COUNT         8992 a        13832 c        14426 g         9241 t
ORIGIN      
        1 atccgggtgc aggcggttca caccggcacc ggaaagctcg gtgatccggc cggaaccgtc
       61 caccgcgata ccgagtccgg cggacgtctt ttcccgcaac gtttcggacg cggcggcaac
      121 gccaactgcg agagtggcca tcgtgataac tgtgacgagt tgtttcatac gaatcgttcc
      181 tttatgctcg taatcggttc gtgacaggga ggtgcgaaga tcatgcgttt ctggtgaaaa
      241 gggatgcaga gagtcgggag cgcgggcgtc cccgcccgca acaacgtgtc ttgtgctttc
      301 tggttttatc cgcagattta cacagatttc gcagattttt tgttctgccc gcaggacggg
      361 agggacgagc gtctgctcgt ccgggcggcg tttcacgccg ccgcctttga acgccgaacg
      421 cccaacgccg aactcagaac gtcgaagtga aaaaactcct aactcctaac tcctaactcc
      481 taaatccacg cgtagcgcgg aagacgcccg cgctcccgac ttgcgcacac gcaaaccacg
      541 cacaaaactc gcatgcccct gtcacgaacc gattgctcga tcatagctcc ttctgccttg
      601 tcgtttcgtt ctgttgttat ggttctgggc agacgtcagc gcagaatgac ggtcgtgccg
      661 agttccttgt aaaggtaaac cgcgccgccc tccctgacaa cctgccagcc gggctgcggc
      721 tcgtcccagg acggaaggcc ggtaagaact ccgtcggcct cggcaatttt cactttgtga
      781 agggcgtttt tcagcgtccc gaccacaacc agcctgcagc cggtaaacgt caggtcgccc
      841 gtgacccgga gcggattggg gcatccgccg gtttcgtcca gcgtgaccgt ataggtgtcc
      901 gtgagcgtca cgtcaccggt cacctccccg ccgccggtga gccgggcgac aaccagcggc
      961 gcgccccgca gattcaggct ggcgccggcc gtgagggtga cttccgagcc tgcggcgaac
     1021 acttctgtgt tcacggtata tcccggggtg cgccatttcc gcgcgagata atgctccaac
     1081 tgatcccgtt cggctttcgt cagcatccgg ttgtacgcga tgacctcgcc gatcgcgccg
     1141 cggaaacgtc gcgtatcggt gggggagttg acgtagaaac ccccgatggc gttgttcccg
     1201 gggccggctt tcaccggcga ttcgatgtgc gccgtgaccg gcgtgttcag ggcgtaatcg
     1261 aaaccgctgg cggaagccgg gagcgcgatc ccgttgtgcc acgcctccgc gccgacgaaa
     1321 cagttgccga ccgtcccgta tgagaaattg gtcgaactgc ctcggactcc gaggtcgccg
     1381 ccggagttga cgccccaata cccagccata ttcatctggt tctgaaacgc ctggaacacc
     1441 atgaccagcg tctgaaccgc gatggatttg ttggagcgca tccagttggt ggtggcgttc
     1501 ccgaaaaaca ccgcgctctg cccgttgatg cttccggtgt aggtgggcgg ttgcgaggtc
     1561 tccgcgtccc aagcctgctt ttcgaggccg aaggccagtc cttcggttcc tgctttggaa
     1621 acccagttcg tcacatagcc gtctgccgag cgccgtaccg ttgccgcgtc cgtcgcgtcc
     1681 agccagtact ggaggccgtc ctgcggcggc agatcattga agaccgtgac cgccgacgcg
     1741 ccgccgaacg agaccgtccc cgcttccacg cgcagtgcca tgtccgccgc gccgcacagc
     1801 gcgaggttga ccttctcccc gatggtgccg cggaatccgt tggttcccgt cacggtgagc
     1861 gtggcggtct ccgcgccgct gttgatgatg tttccgccga tgccggacag ggtggagaca
     1921 gtctgatcta tgccgttcag gtcgaacgcc gccccgggtt ccacgctcag ggttgtcccg
     1981 gccggcaggg tcccgtgcac gggtgcgggc gcctccgcgt gcagttcggt ccccatccac
     2041 ttttgccaca tataattttc gaccgccttg atctcgtccc ggctcagaat ccggctgaag
     2101 gcaatgacct cgccgatgaa cccgcagagc gaacgtccct ggtaattgtt gccgatcacg
     2161 ttgtttcctg cgccgaaatc atcggtgcgg aaggcggaga gcagcgtcgg ctcgttcggc
     2221 gtgaacgtat tgaccccggc gacgccgttg atgctcaggt ttgccagcgt actgtaatgg
     2281 aacacgctgc cttctgtgcc cgtgtcgatg gaggtggcgc tggcgagacg caaaaaacgg
     2341 ctgccatgat caccgcgcgt gtttgcccag aggcagtcca tgccgccacc ggcaacactg
     2401 tacccggtcg tctggaagag gatcagcacc gtcttggtgg caaaggcctt gttcgccacc
     2461 aggcagttgg tccaaccgtt gaaatagatt gcgggcttgg actggatggc gccgccgaca
     2521 ccgttggagt agaccgggca cggcaggcca tcccaggtct gtttgaaggc gacgcccgtt
     2581 acccggtcca cccagcccgt cactgcgccg gtcgtttcgt ccgtttgcac gctggccgcg
     2641 tcgttcgcgt cgagatgata cgcgatgtcc acctcgcacg ggatcagctc ccggtacagc
     2701 ttgagcgtcc cctcccgaac caccgtcgga ccggaataac tctgtggttt ggcgatcccg
     2761 agcgtgccgg aaccgatttt ggtcaggccg atgaggttgg tgccgttgtc gcgaaggtac
     2821 cctccgtata ggatgtccga cgacgttccg atctccgcca tggccggcgc gtccgcgcct
     2881 tcggcgatcg gcgtgatcgc gcgttcggaa ctctgggcca gattgcccag tcgggtcgcg
     2941 gcaccggcga ttccgatatc cccttgtgtc gtgagcccgc ccatcgtcac cgaagggctg
     3001 gcgaaaagaa gtgtgttcgt cccgctgttg atccaggatc cgacaccgga aaccgcgttg
     3061 gagataacga aatccgcatt cttgttgacc gtgatcgtgg tgttgttgat cacctcgcct
     3121 gcgccgaacg tgccgttggc tccgagcgtg agcgtgccgg tctccagccg ggtcacgccg
     3181 agataggtgt tggtgctgtt cagagttaat ttattggaat cctgcaggat gacgagtccg
     3241 ccggggccgg tcaccggaca gtcgaattgc aggtttttgt tcatccggat tccggcgtcg
     3301 tccgccagca ccacccgggc gatgttcgtc gaaaagtcct cgccggtgcc ggtgaacacg
     3361 gcccgcccgt ggtaagtgct gatgccgaat ccggcaaggc tcagttcgct gatcgctgtc
     3421 ttggtatagt tcaggaattg ggcgccgcag aggcggtcgc cgaagacgcg gaccgcgggt
     3481 gcggaagtga ggtccgcgta aagctgcacc cggccccagt aggcccggat gtcaccgctg
     3541 ttggcatagg tgccactcaa tatggtgttg acgtaatcca cattgttgcg cccggtgaac
     3601 gtgacgcccg ccgtgccgcg gatttcggta tcgatcgtct gtagtgtttt gctgttgttg
     3661 tcctgtaccc agacgatgcc ttcggagccg cggaaatcga tcgcgccgcc gctgatgcag
     3721 gtcttggtgg tcgtgaaccc ttgcaggatg atgccggcgg gcgacgtgcc gtcgccgatc
     3781 gtcagcgtca cgccggggtt gagggtcagc accgcgttac cgtcgatgac cagcgcgtgg
     3841 atcgccgtgt cggcggaaag cgtggtgttg gcggtgatgc gcacgatcgc attcgagtcc
     3901 gctccggcga acccgtcatg gtagaggtcc gtggcgggca cgagcccgtt ggacgcgtca
     3961 taccgcgcga agcacaccgg cgtgctgacg gtgttcgggc cgccgagggc gatgaccggc
     4021 gagaccatgc ccgagcccgc gtggaccagc gcggccgggt ttgtcaggaa gaacttctct
     4081 gaagtgccga gcgtgcccgt gccgccgttg ggggccagat acagcgtgcc gggaaaaaca
     4141 tcctcgcgga cgacctcgcc gattgtgagg gaagcggagg cgttggagcc ttttttcagg
     4201 ctgaagcgca ccgaaccctt gagcgcgacc gcgctgtttg tggcgatcgt gagtgcggcg
     4261 gcggaaccct cggccgcaac gggttcaaac ctgatttcgt tgccttcgaa cgtgacgcgg
     4321 ccgtcggtga gcgcggtgcc gcttttcgca taaaccgcga ccaagccgtg cttgaacgtc
     4381 acgttggtga atccgctgta gaacgcgccg tcatatacgc ggagcggtcc gcggcccgtt
     4441 ttctcgaacg cgttgttgtt accgaggaac gggattttct ggtcgacgta cttgccctcc
     4501 tcgtctttcg gacgcagata cggcgcatcc ccgacgaagg tgatcggctg gttgccggcc
     4561 agcagaaacg cgccggaata cggaaacgcc ataccggaaa tctcaaggcc ggccacatcc
     4621 tgatacacgc cggcggaaac ccattgactc atgctgtcgg acgccgaggc gtgggtgaac
     4681 acgcccccgt tctgcgcggg cacgccatca agccaatact tggtgttgct ccatttgagg
     4741 ctgtcggggg cgtatgtcat ctggttgacg aaccagtatc cgtccgaggc cgaaaccgcc
     4801 gccgcgagag cggccgtcgt gatgaccgtg atgagttgct tcatgtgaac cactcctttg
     4861 catacacagg tgaacctctg ccatcccaca ggttgccccg gcaccgtttc ggatcgtatg
     4921 accggcatat atacggcgag ggagaaacca tcccaagtgc cttataattt actcgtttcc
     4981 gcacgcgttt gtccagaaca tccaatgtgg gataggtaat cggtttgtga cagggggggc
     5041 gaaggcaatg cgtttctggt gaaaaggggg gcagtgagtc gcgagcgcgg gcatccccgc
     5101 ccgcgttccc gactttctgt ccgttccgcc cattttcatt tcatgaagtt ttatgtgttt
     5161 catggtccga aaacttgagg tcggcgttcg atccggtcga gtcggtcttg cggcgccgat
     5221 catcctgtga gatactgaac agactttaac gggaccggag gagtgatggg atggttccgg
     5281 gggaacaaca agggaggacg catgcgtttc gcgatgatgg ggatggtgtt ggggttggtt
     5341 tccggtgtgt gggccgaggt cacgatccat gtggcgccca atgcgcctgc cgggggggac
     5401 gggcggcggc gttcgccgta cgcgacgctg gagcaggctc gggatcatat ccgggcggcg
     5461 cgtaaggagg ggctgatcgc gcgggatgag ccggtcacgg tgctgctgga gcccggggtc
     5521 catacgctga cgcaatcgtt tgaactcacg cgcgaggatg gcggcagcga ggccgcgccg
     5581 gtggtctacc gggccgcgaa gcggggacgc gcgcgcctgc ggggcgggct gacgctgccc
     5641 gcggacgcgt ttcagccggt cacgaatggg gcggttctga accggctcga ccccgccgtg
     5701 cgcgagcata tccgggtgtg cgatctcacg tcgcgcgttc ccggcgggtt tgaaccgttc
     5761 aagaacgctt attcgggaac gccggccgcg ccgtggctgt atcacaaccg caagccgttg
     5821 acgctggcgc gctggcccaa cgcggacgcc ccgcagggag ggtgggcggg tttcaccaag
     5881 gcggtggaaa cgggcaagcc gattccggat tccccggatc ccgctctgcg aaagccgcgc
     5941 ccgggctcct tcgagttcgc ggatccgcgg ccggcccgct ggaatctgga agagggggtt
     6001 tggctgatgg ggtattggac gcacgactgg tcgggcgagg tgatccgcat cgcgtcgtac
     6061 gatccggaca agaaagtgat cgccctggcg gccccgcacg ggtacgggat cgccgcggga
     6121 acctggggca gtgccgtgcg gcgctttttc gcgctgaatc tcatggaaga gttggacgcg
     6181 ccgggcgaat ggtatctgga tcggacaagt aagcagcttt acgtttatcc ggagggggag
     6241 acggaggagg ggggcgaatg ggtcttggcg acgcttacgc agcccatgtt ggctgtctcc
     6301 gacgccgcgc atatccgggt gcttgatctg gcgtttgaat attcgcactg cgcgggcgtg
     6361 gtgatcatat tgaaaaacag cgaacaggtg gaagtcgcgg gatgcgtggt ggcgaatcac
     6421 gccgggagcg gcatcagcgt ctccggcagg gccaacacgg tgcggtcttg cgatctgtac
     6481 caactcggca aagcgggcct ctccctcaac ggaggcgacc gcaaaaagct gatcgccgcg
     6541 aagaatctgg cggtcaacaa ccacatccac cactacgggc tcttccagcg gacctatgcg
     6601 gcggggattg cgctgggcgg ctgcggacag atcgcgcgca acaactgcat tcacgatgcg
     6661 ccgcacaacg ccgtcctcta cggcgggaac gagcacctgc tggagttgaa tgaggtctac
     6721 cgcgtggtga tggagacggg ggatgcgggc gcgttttaca cgggacgcga ctggaccagc
     6781 caggggaatg tgctgcggca caatttcgtc cacacgctgg gcaggggcga tgcgaaacat
     6841 gtcaacacca tgggttttta tctcgacgat tgcgactgcg gcgaccggat cgagggcaat
     6901 gtgttctggc gcgcgggccg ggcgatcatg atcggcgggg ggcgcgacaa tccggtgatc
     6961 aacaatctga tcgtggagtg cccgatcggg ctccacatcg attcacgcgg aatgacctgg
     7021 aagtactgga acaacaccaa cgacccgaac tggcgcttgg atgccaaagc ccaagagttc
     7081 gactaccgga acccgccgtg gagcgtgcgc tatccgaaac tcgcttcgat catggatgag
     7141 tgtccccgcg aaccgctcaa caacccggcc cgccgcaacg tgtttgtgga ttgcgtcgac
     7201 acctgctcgt tcggcggcaa ggtgatggac ctgctgcaca agatggagct tgaggacaac
     7261 ctgatcgtca acacggtcgg cacgaaccgg gtggggaaac tgcgtgaggg gatcaacaag
     7321 gggttccgga cggtttcggg aacgccggac aagccgttcg atccgggatt cgaggatgcg
     7381 ccgaacggga atttcgcgtt gcgccggaac gcccgcctct ttacggaact gcccgccttc
     7441 gaacgcatcc cgttcgagcg gatcgggctg tacaccgaca ccttccggcg taagctgccg
     7501 cgccggtaag cgcgcgtttc accccggcct gcgggataac cgcaggccag agagagattc
     7561 gatcgtgcag gtgtgcgttt ggccggcact catggcagcg ggtcaccgta cagggtttgc
     7621 ggtgccgcac gggtgatgcg cacacgcacc gcatcgcccg ccgccagatc ggcgggggga
     7681 tcgaagatca cgatcttgtt gcccggcgtt cggcccgacc accgcgcggt attccgcaga
     7741 ctggggcctt cggccagcac ctcctgtatg gtgccgacga gttgacggtt gagtgccacg
     7801 ccacgcagat cctgatccgc cagcaacacc tgattgcgcc gcatcttctc ctcctccgcg
     7861 acatcatctt ccagtgcggc ggccggggtg ccgggacgcg gcgagtattt gaagatgaag
     7921 gcattgtcaa acgccgcctc ccgcatgagc gagcgggtct cttcaaaatc agccacggtc
     7981 tcacctggga acccgacgat cacgtcggtc gtgagcgcaa ggtccggcat ggcggagcgc
     8041 agacgggcca ccgcgtccag atatccggcc cgcgtgtacc cgcggcgcat ggctttaaga
     8101 atgcggtcgg aaccggactg gacgggcagg tgcagatggt gacagagttg cggcacgtgg
     8161 cgcatgacgc ggatgagttc atccgtgacg ccggacgggt gactcgatgt gaaacggacg
     8221 cgggcgatgc cgggcacggc ggcaaccgcc tccagcagac gggcgaacgg ctcgcggaag
     8281 cctcccggcg agggagcgtc cgccgcattc cagaccgtgt ggacacggcc gtagttcatg
     8341 acgctctgtc ccagtaacgt gatttcgcgg acgccgttgc gcgtgagcgc gtcgatctcc
     8401 gccagaatct cgcgggcggg gcggctgtac tcggtgccgc gcacatccgg gacgatgcag
     8461 taggcgcagc gccggttgca gccgagcaga atggtcacca gcgctgagaa gccggactcc
     8521 gcatggctgt ccggcacgtc cggcagttcg tccggcgcgg accattcacc cagtccggtc
     8581 tcgccggcca gcacacgggc caccaaacgg gggatcgcgc cggcacggcg ggtgcccacc
     8641 gcaaaatcca gaccgcgcac acgcgtgaag atctccgctc ccaaacgctg cgccatgcag
     8701 cccatcacgc ccacgatccg ttctggctgt tcgcgtttcc ccgcgcagag cagaccgagt
     8761 ttgccgaggg ccttgtcctc cgccttgccg cggacactgc aagtgttgac caggatgagg
     8821 tcggcatctg cctcgcggtc aaccagcgtg tggcctgcgg ctttcaggac cgcggcagcg
     8881 gcgtcggaat cgcgcacgtt catttggcag ccgtaggtgt ggagcagaaa acgcatgggt
     8941 cagggtttgg gttgcgaaac ggttccgggc gcatccgaaa agccttccgt gatccgttcg
     9001 atgaggttga cgtaagtcca ttccggttgt tcaagcgtgc cgaacacccg gaactccaac
     9061 aacagccgcg agaacggcag ggtcatcagt cggacaatgc gtccggcaat cgtcttttgg
     9121 cggaagatgc tgacgcgcac cgtgaagtcc agtttgtccg tgttgaggtc ataggtgccg
     9181 cgtccccgga tgctgacgag atctccctcc agcaggaggt tctcggtggc aaagacgccg
     9241 ttgcagaggg tgaaatccat agagcttgat gattgggtga cgagcgagga gatgccggga
     9301 atgttgcgcg tgaggtaatc ggtcaacccc gcgaacagcc gcatgcggtg gatcacgccg
     9361 ttacggatat tcgcatggcc gctgccgttc aatcccgcga cggtgtcccc gtaggccggt
     9421 ccggtgagat ggaggttccc gttgaccaga ccgacgcgtt cgttggtcac attaaaggct
     9481 tgcgaaattt cggcaagctc cactccggta aagcggatat cggcagagaa gacggtggcg
     9541 gtggcggcat actcggggaa cgtgaaggtg acttcgccgt tgacctggcc tccgcttgcg
     9601 gaacgcgccg cagcctgatg gaaacgtgcc gaatacccct gcatgtcgaa gtcggcggtc
     9661 aggtcacgga ctccgaagtt caaaatcgtt cccttgtcga gcgcgactcg gccgcggaga
     9721 tcgttggtga tcgtactctc ctgtgatgag agggcgattg ctccgcggac cgagagggag
     9781 gggggggtgt cgcagcggat ccggtccagt tctccccggt taagcacatt gatgatcgtg
     9841 gtgaaggggg gcagatgcat cacggttcgg gcatcgatct ccaacccttc ggtctcctct
     9901 cgataggcga ggcttcccgt gattggcgcg ccgtccgcgg tttgcgcctc cagcggatcg
     9961 atcaccacgg tggtgtggat gttggtgccg tacaggaaga gggttccttt ggcataggag
    10021 accggcacgc cgcgatacgc gcaggggccg acgtccaaac ccagccgcat ggcgaagtcc
    10081 gtggaatcaa tatccacgtc aaacgcatac acggcctcga ccggccgcgc gatcttggag
    10141 aagcagttga tctgacggat cgcgccccgc gcgtgcaaca tctcgaaaag cggcatgagg
    10201 ttgtcgggga acgccttgcc cgcgaccaaa ccgctgacgt gacggcgggt gaggtcaagc
    10261 gtggcctgtc cggtgacggt catgccgaac acgttgtcgg gccagtgaat cagcgcctcc
    10321 gacaccgcga cccggttcgt gtccacatcc acgttggcca cgaggctccg gacacggagg
    10381 cccagcacat cggcttgctc gatgcgcagc ggaaacgggg ccaggtcggg caactccggc
    10441 agcgaatgcg gggggtcgga catcgggggc ggtaaatccg ggaaagtcag gtttttcagc
    10501 gtaacgccac gtatgcgcgc ctgcatggga gtctgcggca acagcgagag cgccaccgcg
    10561 acttcctcta cggcgagcag cggaccgtcc gcgatgcgct tgggcagagc cttcacatgg
    10621 agcaaccgca gccctttgtc cagggtgaag gtggcgcgcc cgatccgcac atgcacgtca
    10681 tccgtcgaaa ccgcctgcgc gagtcgttgc accaacggcg cggggatgtc atggccgatc
    10741 aggtacagca cgagaatccc ggccagcacg agaatcagcg ccaaccagaa gagaatcctg
    10801 cacactctgc ctagaataga gaggagtcgc cacatgatga gaattcaggc cggaccggac
    10861 acacccgatc ccaagacaaa gcataccgga agccctcgga tagccgcaac aagaaagggg
    10921 ggaacgccca acgcgcgttg cctcacaatt catgacaccg gaaccaaaaa ggttcgggga
    10981 ggggacgttg cggctggcgg ctgaggtgcc cgtgggaacc ccttttgccc tttgaccaga
    11041 atacgttgga aacgaaagaa aaaatcaagg caggttctgg gttttagatc tcgttggtca
    11101 tacggatggc tgcccacttc tcatgatatg tgggcagaag agaatcgaaa gctgaatcag
    11161 cgtaaaggtg gagagaggga atttgggttt ccagaaactc ccgtgccaga ctggaaaaaa
    11221 aggagggact attcctagca acgatgatgc actgaacata ttcgcctttg gctcagggga
    11281 ccggagatcg ctgacgattc gagaaggaat caaaaccgac gacaaagccg actgacatcc
    11341 ggtatcgttt tggtatataa tatataccgt gaatgttgag ttcgatccag acaagagcgc
    11401 ggcaaacttg aagaagcagg gcaccggctt tctcgaagca caggcgcttt gggaagatgc
    11461 cgacagactt cttgtgcccg cgagaacgca gggtgggccg agacacatgc tggtcggcaa
    11521 gacggggcag aaacattggt cagccatatt cacttacaga ggcgaggcgg tccggatcat
    11581 atcggttcgc cgtgcgcgaa aagaagaggt ggacgcctat gaaaactgaa gtcttggata
    11641 aggcctttga caacggcgac gacgtgtcaa agtatctgaa tttttccaag gcaacccgcc
    11701 ccggaagaga gctgaagcga gtcaacgttg attttcccat gtggatggtt caatctctcg
    11761 acaaggaagc ccaacggctc ggtgttcccc ggcaggccgt cattaaggtg tggatcgctg
    11821 aacggctgag agtcaggact cacgcttgaa atcgtccgaa cccgtcggag cagagacacg
    11881 gggaaaagtg cgttaggtaa gatgagaagg ctgttctcct tgtgaaaggt ttctggcata
    11941 atgcctgaaa ccgttaccgt tacggtaaaa cgcactcaaa ggtgcacaca aggagaacag
    12001 caatgaagaa ggttacagca ataaccaaaa aggttcgggg aggggacgtg cggctggcgg
    12061 ctgaggtgcc cgtgggaaaa ccttttgtcc tttgatcctt gcatgaagaa aaattcgctg
    12121 aatcaaggtc tgaccgctgc gaagaaagcc aagcaggacg agttttacac ccggtacgtt
    12181 gacatccaga aggaggtcga ggcctatctg gagtttgacc ccgatgcctt tcgcgacaag
    12241 gttatctact gcaactgtga cgacccattc gagagcaatt ttttcaagta tttcgctgcc
    12301 aaattcaaca cgctcggact caagcggctc atcagcacca gctacgacgg ctcacctatc
    12361 gcgggccacc tcacgttgtt cccggagcac aatgaaggcg ccggcaagcg caagaagccc
    12421 gacgcgctcg ccgtgatcat cgaccatgtg aaagatgagg acggaggcgg cgcggccaat
    12481 gtggccgacg ttgagttgtt tctgaagcgg aacaaggccg ctcggatcgc cttgaaagga
    12541 gacggcatgt atccgggcgg cgatttccgc agcgccgaat gcgtcgcgtt tttgaaagag
    12601 gcggacatcg ttgtcaccaa cccgcccttt tccctcttcc gcgagcacgt cgcccaactc
    12661 gtggaacacg ggaagaagtt tctgatcatc gggaacacca attcgatcac atacaaagac
    12721 attttcccgc ttatcaagaa caacgggatg tggctaggct gcacgaactt caacgtcgga
    12781 atgttttttg aggtgcctga tcactgggga cattttcatc acatcgatca ggagacgggg
    12841 aaaaagatcg cccgcgtttc ctcttcgtgc tggtacacaa acatggacca tggccgtcgc
    12901 catcaaccat tgagcctgat gactatggcc gagaatctga aattcagcag gaatctgaga
    12961 ggaaagacca cttatgaccg gtacgataac tacgacgcga ttgaagtcgc tgcctataag
    13021 gaaatcccaa gcgattacga tggggtgatg ggcgtcccca tcacattcct cgacaaatat
    13081 aacccagacc agtttgagat tgtgggcacg acagagtcca acgatccgaa caatgcctgc
    13141 aggacgcggg tatatacgtc ccaagagtgc agagatgcct atatggcaat ttttggcaaa
    13201 ccgggcgttt acgacctaaa cgcatccgga gtcgtaaatg gcgtcaaggt ttataagcgc
    13261 attctcattc gccaccgcgc gttgcctcac aattcatgac accggaacca aaaaaggttc
    13321 ggggagggga cgttacggct ggcggctgag gtgcccgtgg gaaaaccttt tgtcctttgg
    13381 tcctttgaac gccgaacgcc caacgccgaa ctcagaacgt cgaagtgaaa aaactccttg
    13441 cctataaatt gacaggatga caggattttc aggattaaac aacatcatgt taatcatgtc
    13501 ctaaacttat tccaaggttt tttcgcggcg aagccgcgcg gcaggctaac tcctaaatcc
    13561 gcgcgtagcg cggaagacgc ccgcgctccc gactgcttac gcacaaaaca cgcgatcgac
    13621 ccgcatgacc cctgtcacga atcgattgcg aatccgttaa atactggcct tgagaaagat
    13681 cgccaagaaa tacaatacag tccagaacgg aaggacatca caatgactac tccaaattca
    13741 acagttcgtg gatcggtgtc cttagcgcct ttacaaattt ggcaatgttg cgtaacgtga
    13801 tatttttttc gccatgttcg atcatgccga catatgtgcg atgcacattt catgaagttt
    13861 catgtgtttt atgttttgta gattcagacc cgttgcccca gtccggaagt ccttcaggaa
    13921 tccactggac gatctgccgc agtacggtgt atactgtctt tatcggcttc gtggttgcac
    13981 ggtcatgcgg cttggttttg tttttcatgc gcctgcaggt tacaggcgga cggctttggg
    14041 gccaacgttt tcgttttttt aggggtgacc ttttgccttc cctacaaaat gtgtgccaat
    14101 caatgggagt cgatgggttg ccggtctgcc ctatgggatg gtagtgaccc ctgtaagacg
    14161 gtgatgagag gaagcagtga aatgagatac agtaaggcag tctgggcgat caccgcagtc
    14221 gtagcggtgg cgcgttccgt tttcggcgtg aattggaagt cggaggtgac aagcggcgcg
    14281 tgggataacg cggcgaactg ggagggcggg gtggtgccgg atgcggtgac gcctgcgtcg
    14341 atcgagaata gcggcgtcga ttattcgatc acgttgaacg cggcggggac gacctgcgcg
    14401 acgaatacgg tgatcggcaa tgccgaggga tcgacagttt tcacgattca ttccccgctc
    14461 agtttcttcg attttacgag ttcctcgccg aagtattact acaagcagaa ctttggcgcg
    14521 aagacgatca tcggcgcggg cggagcgttc aacttcctcg cctccacgag tgacgggttg
    14581 tcctccgatc aaggactttt tatcacgaag tccgagttcg ccgtcgatgg cggcaaggtg
    14641 gatctccgga tgaacggcca tgtcgcgctg agcggcacgg cgcagcaacc tgcgacgttg
    14701 acgatcacga acggcggctc cttcgcgtgg cactcgtcag acggctacgg acagttcaag
    14761 gtgccggaga atgcgaaggt gcgcgtccat gacggttcgc tggcgttctc ccagaatcga
    14821 aatcactacc acacggcacc gttttcgatg accggcggcg agatggtctt cagcggcacg
    14881 agtgtcttct cgaattcgta tgtgtccgcc gagggttata cgtctgtcgc gctcgagata
    14941 cgatccggca agatggaatt ccacgacgaa gcggtctgcg tcgtgcaaag caaagcgggc
    15001 gttcacagcg atttcctgct gattccgtcc gatgcgggga agcgtgtcga actgacggtt
    15061 acggatcagg cgtgtttcgg ggtgggagtc gagccgtatc tgatgacggt tggtgaatgg
    15121 acaccgggag ggatcgccgt cgccgagttc tcttcgacag agcagtgcaa tctcggacgg
    15181 actacagccg tgggtgccta tgcgggctac ggcgagctca atctgcgctc gggtcttatc
    15241 gtgctgggga aaacctacgg tcttatggtc ggtacgacgg ataatcaggc gctgaatcgt
    15301 gcccgcgggg acaatgcggc acatcagccg gccgatgcgg cgacttgggc accgacgggg
    15361 ctggtgcggg tcgcgggtgc aaatctttat ctcagtacgc cgtttgtcga caccggttat
    15421 ggttgcaagg ggcttgcggg cttccgcatc ggtgacggcg tctacgcccg gtacaacaac
    15481 aaggccgtgc cggccccggc ggtaggcacg ctcgagcttt cgtccggctc aatcacgaat
    15541 gcccagggtt actggtcgat cggcgcgggc gacggttacg ggcgcttcgt gcagaccggc
    15601 ggtacgcttc tcaagtgcga tccggattat ccgacgtatg gcgggaacca gacgatggtc
    15661 gtcggctttg tgggcgggcg cggcgaatgg gtgatgtcga acggaacggc tcgggtcatc
    15721 gcgccgatga tgatcggcgg cgccgcgccg gacgatttct tgtttgctga tatcaacacg
    15781 ggatggtgtc cgttctccag ccacgatgcc gtcggtacgc tcacgatcgc gtgcgcggac
    15841 aaggagaagc catgctctct cgaggtcatt aaggccaaca ccggcaatac ggatgttcag
    15901 ggtgtctggc tcgggcgcga cggtaccggc acgctcgaga tgatcggttc cggcggttcg
    15961 ctcaagacgc cctatctcat tctcactaat ggttacgaga acgcgagcgg cgaacttttg
    16021 acccacggca aggcgaaact gcggttcgtg ctcgacgagg acggtatcgc ttcgctcacc
    16081 gtttcgacta agatggcgat tgcggataac gcggagatcg aagtcgacgg ccgcgcctat
    16141 accggcgaga cgaaaaaggt gaagctcgtc aacgtaaccg gttcgcgcag cggttccttc
    16201 cgtcccgaga acatccgggt caagggcggc gtgctcatcc aggatgagga caggcatctc
    16261 tatttccggc acatgaaaac cggcttgagc gtcatgcttc gttaaggatg cgttcacaac
    16321 atgaaatact gcgcgatgat ggccgtgatg gcggctgtag gaagtgctct cgcgggcggc
    16381 aatctgctga cgaacggaga tttcgagggc ggcaggctca cgggctgggg aaattgcgcg
    16441 agcccgggcg gcagcacgat gagcgtcacc agagatgccc atgccggggg gtacgcgctc
    16501 gagtacacgc ataatggcat cggcaatcag gcgttctgcc gtaatttcgt gaaggtcgtg
    16561 ccgggcgcgg tctacacgat ccgcggctat ctgaaggggg atgccgaaaa gatcaatatc
    16621 cggctcttca attatgccga ggggatgagc tacgtcggcg agaccgatat ctacacagcg
    16681 acgaaactcg aggactggac atatttcgag aaggcatttt ctgcgccgga ggacatcaag
    16741 tcggttggtg tcgggcttct cctcgtcaaa gggcgcgcgt accttgacga tgtcgaggtg
    16801 cttgagggca atcagatcgc ggaagagcca aacgtcctct ggaattcgtc cttcaaactc
    16861 tcctatcatg acaacggtat gcccgagttt tggaacaccg gcggtggcca cagtttccag
    16921 accttccagc agggcacaca tgaaggaaag ttcttccgca tggccgatga cgtcgcctcg
    16981 ccggtggagg gcgcgcgtgt tctctacgtg aacaaggtcg gccgcacata tctgaggccg
    17041 ggccccctcg cgggcggcac ggactatacg ttttcggtct atgcgcgggc cgtgacgggt
    17101 gaggcgaaga tgaatctcat ccttaggggc atgaggcccg gcaagaaggc gttcacggtc
    17161 ggttccgagt ggcaacgact cattttcacc gcgcatctcg accgtgcggt cgcggaggcg
    17221 acggcgacgc ttgaatacaa ccggaacgaa ctctatctcg ccgcgcccat gttgcacgcg
    17281 ggaaagaccg cgcttccctg gaagccttcg ccggtcgatg cgacctttcc cgatcttttc
    17341 gcaggcaatc aggttccgcc gccgccggcg aagatcgaga cgatcgccct gccgcgcggt
    17401 ggcgactggt ccgcggccgt gaagatcgac cgcttttacg tccacggcga ttatggaaaa
    17461 cacgtagatg ggcgctatca agccgcagcg taccgggatg gagatgtcct gaaggttgcc
    17521 ttcgagatgg cgagcgggaa aaagcctccg gtaaagagca agccgcacgg gccgctcgtc
    17581 accggcaacg aggccgtcga gcttttcgtt tcgccgcagg ccaacggcgc cggagcactt
    17641 catttcatgg gtgggcgcaa cggtgcgtat gcgtgtcagg acaacggtaa cgggcaggct
    17701 gagatcaacg agggatgggc gtttgccgcg agtgagaccg cgcagggctg gcgcggtatc
    17761 ttcgagatac ccctttcgct cttcacgcaa cctggcggcg aggtctggcg catgaatttc
    17821 gcggtgaccg agtttgacgg cgtaaagccc ctttatttca gtgccatcag ggccggcttc
    17881 cattccgcgc ccgatttctt ccgcgtgacc gggctgccgc gaggcagcaa ggtagagccg
    17941 cctgcggtat tcgtggaata cgatttctac acgaccgaga agacagcgcg gtttcggctg
    18001 aggaatgcga aggctccggt ggagatggag gtgacagggg caagcgtccc gcttgctcaa
    18061 gacggtcacg tggatcacgt gacgctgacc ctttcgggaa atgaggggga aatcgaaatc
    18121 ggtgcattcg ctgacggtta ttatgaagtg gtcgtcaagg cggcgaacgg cgagggaaga
    18181 agccacttcc gcaaattcgc cgatgagaag cactatactc gcattaatcg cttcaacgaa
    18241 acgatcaacc acgacggtca gccgtttttt ccgatcattt tcacggcatg gatgggcggc
    18301 ggacgcaacg acaagatccc gactgactgg catatcgggg aattaaagaa acgcaatttc
    18361 aacacggtca tgatcccgaa cgagctctac gtcttgtggg acacgcgcaa ctcgtcggag
    18421 atgcgcagaa ccatcgcgaa gaagttcacc gatgcgggct tcaagcttct catctggtcc
    18481 gcgtgcggca atctcaagga agaaggtatg atgaaggccg tggttgatta ccgcaaacgt
    18541 catatcgacg aacttgccga gttcaagaag gacatcctcg gctggtatta tctcgacgag
    18601 atcggcgcca actgggaaaa gagcttcggt gtaaagctcg aggactaccc gaagggctac
    18661 cgtgcgatca aggactatga tcccgcgcgg ctccatttca tcaactggaa ccacaccggc
    18721 tgcacggtcg gcacgaagta tttcgcggaa gacggagcga ccgacatcta ttcgctcgac
    18781 tgctacacct attcgtgcaa gccgaacttt gggtcgctct ccgccttcga gatcggcacc
    18841 tcgacgttgc ggtcgcgtat ggacgacacg catctgccgg gtatcgagtg gttgcagacc
    18901 tatgcctatc aggagggcat ccgcgagccg catcagcttg aataccgcaa caacgtctat
    18961 gtcgcgctcg tcaacaatgt gaccggctac atgcacttca tcgacgtgcc cgagcacaag
    19021 gaactctggg agggtatggg cgaggcgtac ggaacggctc tcaaatggct tggtatgacg
    19081 gtcgagccgg ggtcacggct cgtccgccgc ggacgccagg gccagatttc ctacgcgctc
    19141 tgggaacgcg ggagcggggg gcatgctttc atagccgcca acacgctcta cgcaccgcag
    19201 actgaggcga tcgttttcac ggtggaactc ggttcttccg tcgagacggt cggcggcgag
    19261 ggtgctttgg aatcgaacgg ccgctcgctt tccgttgcgc ttcccaccgc cggcagcgcg
    19321 gcttggttca tcacggggag gtgacgacaa ggcacggcat gggatcttgg aaatatgccg
    19381 atttatggga ttgacggttt tcaccttccc tgcataataa cgggtatcgg taacgggaat
    19441 cgaagtgtcc cggcctgtcc gtctactcaa ggagatcgtc gttaaggaat atgaattggg
    19501 gaggcaaaac gtcatccgaa atgtccgggg catcacgctg gtgtcgtgcg gactggcctt
    19561 gatggaaatg gctttcggcg ctccggtcct gaccggcgac acgtccaagc ccgcagcctc
    19621 gatcttttat cccaaagacc gggtgtcgct ttattttcac gtttcggggc ttgccgcctt
    19681 tcagaaggac atcacgctga agttgacggt cgaaaacgag gacggcaagg tcttctgggg
    19741 gcgcgattgc cccgtgaagc ctgatgccaa cggacaggac gaagtgatcg tgaccgatgc
    19801 gcccggcggc cgtctcggct tctaccgtct gcgtgccaga ctctcggacg ggacgttgct
    19861 gacgacgccg tgggcgacgc gtcagccggg tcaactgacc tgggccgtgg ttcctgcgcc
    19921 ggaagagcgc gagcccgtgg acgtttcgta tctgcgtttc ttcaattcgg gtgatcctga
    19981 cctcgtgggc cgcatggctg tggatcgggt cggcccgacg aatcctaaga tcgggccgtt
    20041 cggcataacg tcccagtggc ggcatctgga ggcgcatcgt ccgggagaat atgcggacga
    20101 tcccgaagcg gaccgcattc ccttgccgta tgccgtcggg aacctttata cgcactcgac
    20161 ggcctggggc aatgatttgt tggcgacgca tttcaagatg caggggcatg cggacggcca
    20221 cccgccggcg ctgacgcctg cgggcgaaaa ggcttgggag gcctttgtga agaccttcgc
    20281 acgtaatttc cgccgtcagt atccgaccgt gccgcgcgtc tatgagttca cggcggagta
    20341 ctatgcgccg gacaacggca gccgcacgct caaggatatc gtgcgcatgt acgaagtcgg
    20401 ctacaaagcc ctcaagagcg aagatccgga ggggcttgtg gcagggccgg gattcggcta
    20461 tgccgccaag gactaccatc aactggaatt cctcaaggca ggcctggctc gctggctgga
    20521 tgtgatcacg gaacactctt actacaccta tccggctgag ccgaacggca tcgtcgatgc
    20581 cattcgcaca tcgcgtcagc tggtgaggca atacgccggg cgcgaccttg ctttctacag
    20641 cacggagttc gggtatgcca ccgaggactt cgagccgcgt gaacgggatc agttgcggca
    20701 cattctgcgc gtgtcgctga ttctcttcgg cgaggggttc aaggcgctct cggctttcgt
    20761 gccgagcgac tatcgcctcg agccggggtt tggctacacg tataacctgg cgctcatcaa
    20821 ggatgtcgac cggaaagacg actggttcca caacaagacg tcgcctaagc ccgtttatcc
    20881 ggcctatgcg gctctgacgc atctgctggt caactccgag acgctgggcg acgtgccgtt
    20941 ccttggcgaa acggcctggg gatacgtctt ccggaaggcg aacacgggac gcatcatcct
    21001 tgcgctgtgg gactggagcg gtacgccgcg cgcggtgaaa ctgcccgcgg gggttccggt
    21061 ggtcgatgtg gtggactgca tgggaaattc gcgtacggtg aagactgtgg acggcgaact
    21121 gtcgctcacg ctgacggatg tgccgcagta tgtgacgggc gtgtcgccgg atgtctggac
    21181 gaatccgatg ggtgaacggg cgaagcttgc agcggccttc caggcgcgcc agaaagcggc
    21241 ccgcagtgcc aggggcgtgg aaatcaccgg cgtctcgtcg gcctgcatgg acgacggacg
    21301 ggcggcggtg gatgtgacgc tcgccaatct gcgggcggag acgaacgaag gcgtggtgcg
    21361 cgtgcgcgtg aaagggaatc cggacggtcg cgcgtcggca ccttaccgtt tgtcgcccga
    21421 acagacggtg tccgtgaagg ttcctatggc cgccgatttc gtaggcgcgg cgctgatgaa
    21481 cgaggaggtc gaggtggccg ttgaacagaa ggcgggtccg cgcgtggaat gcggggagaa
    21541 cgtgaacttc ctgttcgcgc cgtatcgccc gaaggtggcc gtggacggga ttttcgacga
    21601 ctggaaggac gtgtcgatca gccgttttga ccccgcgcgg caattgacga acggcaaacg
    21661 gtattacaag ggaccggatg attgcacagc gaccctgtcc gtctgctggg atgaccgttt
    21721 cttaaagttc ctgttcgatg tggtggacga ccagtatgtg ccgccgccag ccagcaaaac
    21781 cgtgtggagc cgtgactccg tgcagatggc tttttcgggc gtctaccatc tgataaagac
    21841 gggcaacatg ctacaggaca agttgaacga ggccgcgacg gagtgcacct ggggactgac
    21901 ggaaaaaggc ccgtatgcgt accgccacgt atcctttgac aacgcgatct gtccgcgcgg
    21961 cccgctggat ctctcgacgc atccgttcca tgcgtcgatt gcgctgtgtg cgaacgggaa
    22021 atcccgcatc cgctacgaga tcgccgtgcc gtggcgcgat atgcgggttg cggagcctaa
    22081 ggtcggtgtg cgtgtcggat gggcctgtac ggtgaacgac tgggatcccg gcaaaccggc
    22141 ctattccgcg ttcggcgcgt tcaacctgaa aatccccgac cagttcggtg ccatcgtctt
    22201 gactccgtga ggaaaaatga tgttcgccac cggtctattc gtcttagcgg ccacgctcca
    22261 gttcaacggc atcatgggcc agtctgcggt tccgggagac cctccgttga cgttcacggg
    22321 cgtgcgggcg ctgacagaag ggccgggcgg ccgccttctg ttgacgggcg acgacggggt
    22381 gctctatgcg ctcgtcgacg gggaggtcgt gtccttgggc gtgagggtgc cggctgcgca
    22441 tctggcgtgg gacggcagga cgctgtgggt gctcggctcc gatgcggtgt ggacggtgga
    22501 tgaggagtgt cccggctgct ttgcggcgca caaggccttt gacgttcccg gcctccattt
    22561 cgatcaggcg gccgttcgtc cggaactggc ggaccatccg ttcgccggga aggggaagtt
    22621 cttccgctgg gatcgagggc gcaatgtgct ggtggctcac gacgcggccg gccgtgaatg
    22681 cggaaccgtg ttcgcttttc cgaggcggaa gggcgacagc gcactggtgg cgctcgggtt
    22741 tctgccgggc acggaagacc tggtggcgtc cagttactat cccgacgtgc gtctgtaccg
    22801 tttccgcgtg gatggctcgc aggtgacggg gaacgggtgg cccgcgaatc gcggttatgg
    22861 gttgttgcgc cgttttaagg gacggctcta ccacatggga accgacgtgt tgccgttggg
    22921 cgaaaacctg ctgggtgcac gcgcgatgcg tgtgggtcat gaagcgcatc atcgcggatg
    22981 ggcgacagat ggtgtgcgtg attatctggc gacatcgcag ggactctatg tgcgagagcc
    23041 gggggaaacg cggtttcgaa cgcggcgggg cggcatccgc ccgctcacgg ctttggctgt
    23101 caacgcgccc tacgtttttc tgtcaatggg cggcacggtg cgctggctgt atctggatga
    23161 agacgcgacg ggcgtctttg cttcgtcgga cgatgacaac ctgcgtgttg ccaacgcttg
    23221 gatttatccg ccgcttgaca tggtgccgga aggagcgaat ctgctggtcg ctggcgggga
    23281 ggccgggctc tactgcttcc atccgtttct gccgccgggg gccagtcccc gcgaccattg
    23341 ggaaaagagg gcgccgggac cgtgtccgcg tgttccgggg tgtcgggaaa cgtttcccgg
    23401 ggtcgaggcg gccatcgtcg ccgcaggtgc cgcgctcccg gagggtatcg aaccgggtct
    23461 ggtggcccgc cagggcaagt ggcttgtcgt ggaggatcgg aagaatttcc gtcttgtccg
    23521 ttttaaggtc ttgaactaaa cgaagaatgg taatgagagg agtaaagcta tgatgggtaa
    23581 taagacgttg atcgcggccc tgttggccgg ttgcggcact gtggtgtttg cgacctcctt
    23641 cccgaatgcg gacggttccg gcgacatcgc atcaccaacg gcttggggag gcgtgctccc
    23701 tacggatcaa gctcagttcc agacgtccga tgtcacgaac acggcatcgg caaacgtcac
    23761 gttcgccggt gtcgaggtcg gtgcgaagaa tgtggttttt gatttttccc agacgcccgc
    23821 ccgtacgatt acactgagcg accattttag accgctcaac tacacgggcg cgtcctatac
    23881 tttgcgcggc gggacatgga atttgagcgg aaagaatttc catctttacc ataattgggg
    23941 gcagggaagt gagtgcacgg tgacactgga aaacgggtgc aacgtcacga atgtcggttg
    24001 gaccctgctt aacttttcga cgctcaaatg ccatttgatc atccgggatc aggccagcct
    24061 gacggtgaat acctacgatt cgaacagtgg tcacggcaat gatgagcctt gtctggaggt
    24121 tctgtcgggt ggacgacttt ccgtgccggc gacaggttcg actttgcgta tcggcgatgg
    24181 cacgacattg gtctcgggtg cgggatcgcg cattgaaagc tacggcgggg cgggtgactt
    24241 cacgttgggc aatacctacc acaatacctt gatccggatt acggatggcg gcgcctttga
    24301 tgccgcttca cggcggatga atctgaattt gaatgatgct acgaacacga caatcgtggt
    24361 ggatgatgac gggacgttcc aatgcggcga ggtctatcct aacggtcgtg ccttcaatat
    24421 ccaaatcggt gaaagggggc agatgacgac ggggcgctgg tatataaagg ggaacgacca
    24481 ttccgtgagg gttttgaacg ggacattgaa agggtcgcgc tggatcggcg aggcggcttc
    24541 tggggcttcg gtcacgaatt tctcggtcgc cgtcatcggc agcgatgccc aggtggatat
    24601 tgcggggggc gatgccttgt tcatgggcct gtcggattcg tcgattctgc tggaaggggc
    24661 ttcctggtcc tgtcccgtaa cctactggaa aagccgtgag tcgtcgaatt gcacgttccg
    24721 tctgacgcag caggcggcgt ttaccactac ggggcatttc tggggtactg cctatgacgg
    24781 gggatcggcg tcgcagacga attgcaatgg caatgtgatc agcgtcgaat ccggctcgac
    24841 gttccagtcc gccgggttca ctttcgtcgg gaaggggaac cgcctgatcg ttaccaatgc
    24901 cacgttccgc tgcccgacag ggtcgttcaa tttcgggcgg tctgacaggg ggtcggacgc
    24961 gaatcaactt gttctgcaag gcacgacgcc gcgtgtggaa atagcaagcg acgcgatgca
    25021 tttgcgttac ggttcaacgt tgcgcgtgga aatgccggcg gaggggtatg cggcggattt
    25081 tgtgccgatc acgacggcgt ggagcttgtc gcttgacgac tcgtgccatc tggtggcgga
    25141 tgtcgaggcg atcaggcggc accagcgcgt gaacaagacg tatgtcctgg ccagagccaa
    25201 tggtggcgtg agcattccgg aatccgtgct ggccgaggcc aatgccgtcg caccggacga
    25261 gtggcagttc ggtgtgagcg aggataacaa atctcttacc ctgcgtgtga tgctgcttcg
    25321 gggaatggct gtcatgctta aatgaaagtt acgatattgc cggagcggga cgcccatgtc
    25381 cccaaaaact ttcgtgccct tagataaacg gggataggcc tgggttcatc cggcctccta
    25441 cagactgcct gtcagcgcgg agagcacgtt ttcttcggtg atgatcccca gcaccgcatt
    25501 gtcctgagtg gcacgtacca gcaccatcgg ttgccggtat gcgcgcatca ggggcaagac
    25561 attgtcggcg cactcgtcgg cgcggacaaa ataggggggg tgcatcccgt cgcgtgccag
    25621 ggtacgcttc ggatccggcg cctgcgcaag gaggctcagc acattcagca ccccaacgca
    25681 acgcgagccg tctgcggaga acaccggcaa ccgcacatga ccggattcgc gcaccagccg
    25741 gtaacactct tccagcgggg cgtcctccac ggttttggtc acccgcgcca acggggtcat
    25801 cacctgctcc gctttcatgg actgcaacgt tagcacgcgg ttgatcatca ggcgctcgaa
    25861 cgcgctgatg cgcgctccgt tgcgtgcgtc actgaccaca tgctggatgt actcgcgcgt
    25921 catcaaaaaa cgctgccctt gggcaccggc tttctgtggc gtcaaccact tcgtcaacca
    25981 aaggaccagt gcggtgagtg gcgagatggc cttctccacg aaatagaaga ggcggatgat
    26041 cattagggtg cggcgcaggg gacgtgtcgt gaagaacagc ttgggcaggt attcgcagaa
    26101 aaaaagcagt atgcaggcca tggtcacggc ccatacgctt tggagcgtgc ggctgttcgg
    26161 cagacaggtc tgcgcgaggc tggccgagag ggttgaaagg atgacgttgg tcaggttggt
    26221 gccgaccagg acggccccca tgaaacgttg catgtcggca aggtatccgt tgagcaacac
    26281 ggcggacttc atccccgcgc gcaccagatg tatcagccgt gcgtggctga tcgacagcac
    26341 ccccgcttcg gtagcggaaa aaaaggctac ggccgcgaaa cagcacaaga tcaggaaaat
    26401 ctggagttcg gggatcatcg cagttccccc tcctccggat ggtcatacgg caggtcatcg
    26461 acctcctgca ggtcttcctg caggtcctcc ggtgtctcct ccgggcgttc caacacctcc
    26521 aactgcacca cgtcgatccg gcgtttgcgc agtcggcgca cggtcacgcg gcatccctgc
    26581 gcctccacga cctggcctgg ctgcggcaga cgtttggcat ggaaggtgat ccatccggcg
    26641 atgcggtcgg catcgtctgc ctccagttgc aggtcgagac ggtgattgag ttcgtcgaga
    26701 ctgacggtgc catcgaccaa ccagatgtcg ccctgcagat tacggatcgc gggtgtgcgt
    26761 tcctccccgt tgcgggctac cggtacggtg atgagttcta aaatatcgcc gcgcgtgatc
    26821 aggcccgccg tgccgccgta ctcgtccaac acgcaggcga tgtgcttgcc gctgcgctga
    26881 aaagtcacca gcaggtcgtc cagcgtcaca ttctccggca cgaacagggc gggggttgtg
    26941 gctttacggg cattgtgcgt gggatcgagc aggaacatgg tggcatccag aaacccttcg
    27001 attgcgtccg gtgtccgcac atatacgggc agatagcgga actgcgcttt gcggacagtc
    27061 tcgacctgtt gtgcgacggg cgtgtccaag tccagtccca ccatgtcaac gcgcggggtc
    27121 atttcgtcgc tggccatcag ttccgacagt ctgaggatgc cgtccaccat cgacgcctcc
    27181 tcggcatcga tcacgccctg ttcctctccc acctcgacca cggttagcag ttcgttatta
    27241 gaaagagcgc ggcgttcacg gcgcaacgcg tgcttaaaca cgcgcgatgc ggcggtcagc
    27301 accagattga acggccgcag cagccagagc cagaagagca aaagtcgact gcataggggc
    27361 gcaagccgct cggcatggag cacggcgatg cgcttggggg tcacttcgcc aaagagcagc
    27421 agcgcgatag tgaccacggg gatggcgatc acctcgcccc attgcggagt caggcgggag
    27481 aacatgaggt agccgatcgt cgcaatcgcg aagttaacca gagtattgcc gaccagcagc
    27541 gtggagagaa ccagcgacgg gttggcaagc agtcgttcca accgggcggc ggcctcggcg
    27601 ttgcggttgc ggatccgctg cacatggatg ggggagaggg agaagagcac cgtctcacta
    27661 cccgaaaaaa aagccgaaag cgcgaagagc gtcagcagca caatcccgtt cagcatgaag
    27721 aaaatcggat tcatggtgag gaaacgtcga tcaccgacag gcgggtccga tgcccgctgt
    27781 tcaacgtctc gatctccatg tcgcggacca tccagcggtc gtccatcttt ttgacgcgct
    27841 gcacccacat tttgcgcacc ggcgttcctt gcgggtcgag ttgttcggct tgcatcaggc
    27901 agcgcaactg ccggtcgacc cagacccgca ccgctgaaca cccggggatc ggcgcgggcg
    27961 gcacggtcac caggatgtca cagtcccggc cgttgcggct ttggccgcgc gggacatcgt
    28021 cgaagcgcac atctgtccac cagaggaaat caagggtcag gtccatccag gtcatgtccg
    28081 tgccgcggac tcgtccggca taggagggtg agtccaccgg ctgctgttcg gggccgcgga
    28141 acaggctgat ctgcgcgggg cggcctgcgg gtcgcgtgag caccgcgcgt tcgaccagcg
    28201 aggtgcctgt ggcatccagc agcagcacct cggcggaagg cgggcgggca ccccagtcaa
    28261 gcatcagctt gaacggttct tccgcgagtt tgatcccgcg cagtttgcgt accgtaagcg
    28321 tgccttggag cagcagccga tccgtgggga tcagttgggt gcaggccgat agaatctggg
    28381 ccgcggtcag gttctcctcc agggccgccg cggctgtggc cggacgcgat tcgtcatcag
    28441 cgcggcagac cgcgccgatc aggatgacac acaacaaaca gacgcaaccg ggtgtgctct
    28501 tcaaacggac ctctcataac acgacggggt tcgagaccgc gtgtatgtct catgtatagc
    28561 atatccccgg cctctccgca acaagcggaa tatcattcgc attgcggtac ggccacaaaa
    28621 aacctatgat accgaatcat gcattcgatt ctcatggagt tcgggccggt caccatccgg
    28681 gcgttcggcc tttgtatggc gctcggattt ctgtttggct ggcggacggc ggtgctgctc
    28741 tgcaaacgga cggggcagga cgcggatcag ctgaccacgc tgctgacttg gctgatgatc
    28801 tcggcgttgc tgggagcccg cgccgcttat gtgctcgagc actggcacgc ggagttcgcg
    28861 gacgcgccgc tgatgatcct gcgtttggat cagggcggtt tgatgttcta cggcggcttt
    28921 ctcgcggcag cgctggtcct gttgctctac gcgcgcatcc accggcaaca tgtgctgtcg
    28981 atcaccgatc tggtgctgac ggtcgtgccg cttggccacg ctttcggtcg ggtcggctgt
    29041 tttctgcacg gctgctgcta cggcagggta accacctcgc gcttcggagt ggcttttccg
    29101 caagggtcgc ccgcttggtg ggagcatgtt tccgcgaagc cgccgctgat ttcgcagcac
    29161 gcgacggcga gcttgccggt gatccccacg cagttgatcg aggccggggc caacgcgttg
    29221 ctcttcgttg tgttgttcac cctttatcca cggtgccgca cgctgcgcgg actcctcacc
    29281 ggttgttatc tgatggccta cgccacgttg cgtttcggga tcgaaatgct gcggggtgac
    29341 ccgcgtctcg ctgtggggcc gttctccatt agtcaggcga tcagcctcgt actgttcgcc
    29401 ctgggactgg cgctcgccgt ctactcgccg cggatgcgtt tggatgtgcg gatcgcacag
    29461 aacactttgc cgcacatgga gcagtgatcg tccgcggccc cgtcctgcgg ggaacgcgtt
    29521 ttgagccagc gggcgcgggc gccggccgga tcgagcgcca acgtgaactg cccctcccag
    29581 tcgaaggccg cgcgcgcgcg ggacatcgca tcgtcacggt tccgtgcgcc gggtctgccc
    29641 cgcgccacat cgccggcatg cgccgcgatg cggtgtgcga tcaccccttg acggacctca
    29701 tctgcctccg gaagtccgag atgttccgcg ggtgtcacat aacagagcag cgaggcgccg
    29761 taacaggcgg cggccgtagc gccgatgctg gaggtgatat ggtcatatcc cggcgcgata
    29821 tccgtcacca ccggtcccag cacatagaac ggcgcaccgt cacagaccgc ctgctggcgc
    29881 tccatattca tttgaatctg gtcgaatggc acatgcccgg gaccttcgac catcacctgc
    29941 acaccccgtg cgcggcagcg ggtcacgagt gcgccgagca cgtccaattc gccgaactgc
    30001 gccgcatcac tcgcatccgc cagacaaccg ggccgcaacc cgtcgcccag cgacaccgtc
    30061 acgtcatgcc gcaccagaat gtcgagcact tcatcccagt gcgtgtacag cgggttctcg
    30121 gcctcgttgc gccgcatcca ctccgccatg atcgcgccgc cgcggctcac gatacccatg
    30181 atgcgccgca tggccagatc cacatggcgc agtaacaggc ccgcgtgcag cgtcatgaag
    30241 tccacaccct gttcggcctg ttcgcggatg acccccagca gcatttcggg ccgcatgtcc
    30301 ggcacgtcac cttcgagacg ggagagggtc tcgtagaccg gtacggtccc gagcggcgcg
    30361 ggcgaagcct ccagcatgcc ctgccggatc agactcacgt ccggtcccac gctcagatcc
    30421 atcacaaagt cggcgccggc atcaagtgcg atgcgaagtt tctccagttc atcgtgcttt
    30481 cccgagcggg tcggcgaacg ccctaggttc gcgttgactt tggtggagaa gagacgtccg
    30541 acccctacgg gacggactcc cgcatgcgcg ggattgagag ggatcaccgc tttgccgcag
    30601 gccacggcct cccgcacctg ttcgggcgtg acaccctctg tctcggcaat ccgggccatc
    30661 gcctcggtga ctgtccccgc ctttgcggat tggagttgcg tcatgtttct ctgtttccgt
    30721 tcacaccgtt tcgaccgtca cccgatcaca cggcagacgc gtgggggtgc ccagcaccgt
    30781 tgccacctcg ccgacctgcg caccggtgcg gatcgattca tacacatacg ccttgccctc
    30841 ggtcaccgcc tcgaccaacg gacgcccgag cgccagcgaa gccgcaatgg ccgcgctgag
    30901 cgagcacccg gttccgtgcg tcgacgctgg cgacaccagt cgcggtgttg tcacacgatg
    30961 caatgccgca ccgtcataca gcacatcttc ggcgcagggc gagtcggcat gtcctccttt
    31021 gaccaacacc gcacagccga agcgatccgc cagttcgcgg gccgcctcct gcatcgcctc
    31081 caacgtctgc accgtccgat ccgaacccag cagtacttcg gtctcggcca aattgggcgt
    31141 cagcagggtg gccaaaggca acagccgcga cgtcaacatc tccaacgcat cctccgcgag
    31201 cagacgcgca ccgctcgtcg cgaccatcac gggatcgatc accttcgcga tccggttgtg
    31261 gcacaccaga cagtcggcca cggcctcgat cacctcgggg caggcgagca tgccggtttt
    31321 gagcgccgtg atgccgtaca cgtcgagcac ggtatccaac tgctctgcca caaatgcggc
    31381 atcggcggtc agtacggcac gcacgccgaa gggattctgt gcggtcagtg ccgcgattac
    31441 cgtgcagccg tgcacgccga aaacatggaa ggcgcgcaga tccgcctgaa tacccgcatt
    31501 gccgccgcta tcgctacccg cgatcgttaa ggccacgggc aaccgcaccg gctccagaca
    31561 cgcgcattcg tcgtttcgct cgtcgctcac tcttccacca ccaccggcag gccgggctcg
    31621 accaaattca ggaaacggac cgcattccag ttggccaacc ggaagcagcc tttggattcg
    31681 gcccggccga tctgctcggg gaacggcgtg ccgtgaatgc cgtagccctg cagcgagagg
    31741 cccacccagg cgaggcctac cgggttgttg ggccccggcg ggatcaccaa cttcgatcgc
    31801 ttcggacctt tcggatcaaa caggtgcggg tcatacagat agttcggatt gggagcgata
    31861 tttttcaccg tcagctcacc gtcagggcgg tgccgcttct cccgcgcgat cgagcagggg
    31921 aagagtgcga tcagtttgcc cgccgcgtcg aacaccgtga cctccatgcg gctaagcgcg
    31981 atgcgcacgg aaccggcttt gacggacggg acgcgcgtag tgcaatcggg cagcacgatc
    32041 tccgtgccga cgggcggatt gggccacgcg acctccgggt tcagacgcgc cagtgcccgc
    32101 tgagaggtgt ggccctgctc cgccaccttc tcctgaatcg tctcgtacgt catgctggga
    32161 agtcgcgccc gttcctccca ggattccgga accggcccga tcgcggcccg gtccgcttct
    32221 gtcaccgtgt gccgggtcag gacatccgca tccccgccca gagcctccag cactgcggcg
    32281 tccggcacac cggtggccgg cagtccgttc aaggtctgcc acgtcatcag ggcgatctgc
    32341 gttcgtttgc cccacatgcc gtccacgcag ttgcacgaca agttgcgacg gtccagcaac
    32401 acttgcagac tcagcacatc ccggttaaag ccggaacggt atcggacgaa gcgctggctt
    32461 gtggccgaag tcggctcggc gcccgtcacg gccgaagcca gactgatgcc cgccagccac
    32521 atgccaacct gttttgcgaa ccccaccgtg ccgaatcctt tccgtttagc aatggcccgc
    32581 attataccac aagcggtagt tcggtcgata tcattcctgc ctctctgtcc ggcgcgacga
    32641 tctgccatac aatcccaatg cgggcggagc ccgtaacccg atttagtcac acacaaaggt
    32701 tttttcatga catcccattt cccttcgtct tcttctattg gcctgatcag ccgatacggc
    32761 acgcatgtcc agacgatgat cggttgcatg tggaaggtgc gtgtcagcca aacagggcgc
    32821 gtgcattacg ggtcgtgatt tccgccaccc gttcgggtcg ggtcttgcgc acagccgcca
    32881 gacaggcggc cacatgggtc acatacgcgg gctcgttgcg ttgcccgcgc atcggcaccg
    32941 gtgccagata aggcgagtcc gtttcgatca ggagccggtc atccggcacg aaggccgcgc
    33001 tggcgcgtac gctttcggcc tgccggaagg tgacgatccc gctgaagctg agggccatct
    33061 ggcggtcgag caactgcgcg gcaaaccgtg tgtcgccggt gaaacagtgg atcaccccgc
    33121 gcagaccctg gccgtgccaa ggcgtctcgt ccagcacgcg cagggtcgcg tcgtcggctt
    33181 cgcgcgtgtg gacgatgacc ggtcgtttcc agacgtcggc cagtcgcaat tgcgccgcga
    33241 agagtgcgca ctgggcctct gcggtttcgg gatgaaagtg gaagtccagt cctgtttcgc
    33301 ccactgccgc gaacggatac gtttcggcga gttgccgcag tgtctccaca aagacctccg
    33361 gttcggcttc ggtggcctgg tcgcggtcga accccagtgc cagacggatc gcatccggaa
    33421 acgcttgggc tgcggcaagg gcgccggcgt tcagtttcgc gttgccgccc accgccacca
    33481 cacggctgac cccggcttcg cgcgcccggc tgagcacggc tacggtctct gcggcggtca
    33541 gggtgaaatg cgcgtgcgtg tcgtaatagg tttcagtaat cacgatggct ccggattgcg
    33601 gggcgtttcc ctgcgcggcg tcacgcgtca cacgggaaaa acgaaacgcc gctcgaacga
    33661 aaaggcgttt tccagatggc ggatcagcgg ctcgccctct tcaggctgcc ccagaacctc
    33721 aatcacatcg aaacggtaat acagatccgg aaaacgcgcc ttccggagat aggccacggc
    33781 cgcgcggttc agcgcgtgac gtttcgccgg attcaccgcc cgcgccggac gggcgaacgt
    33841 ttcgcggtgg cgcgtcttga cctccacgaa cacgagggta tcgccttggc ggacgacgag
    33901 atccagttcg tcgcggcggt tgggccggac attgcggccc agcaccgtat acccgcgcgc
    33961 ggaaaggtac gcggcggctt gctcctcgcc ccatgcgccg acacgggccg aacgcggaag
    34021 ttctttccgt tccgtctctt tgcgacggag gttgagggtg cgctgcagca gtttggagag
    34081 cgcggacacc gcatcacccc gatatagcct gacggccggt atcaaaggcg cggaggttca
    34141 gttcgatcag cgcacccggg atccgttcgc gcatcgcctg tttccagatt tcgggttcga
    34201 acggcaacaa ggccgagagc gcacccgcga tcaccatgtt catcgtgcgc atgttgccca
    34261 tctccttggc gatcgtcagc gcgtcaacat acagcgcgga cggatattcg gcgcgcagac
    34321 ggtcatccag atccgtcacc gcagcctgct ggccgctcga aaccgtgatc ggcgtcatgt
    34381 ccagactgtt gatcagcacc ttgccgtccg gcgcgagcag atgccgccag cgcagcgcct
    34441 ccagccgctc gaacgcgacc agcaagttgc tggcaccgtc cgggataatc ggcgaataca
    34501 ccttttcgcc gaagcgtacc tggctgatca cactgccgcc gcgctgcgcc atgccgtgga
    34561 tttcgctctt tttgacgtcc agacccgcga ggaccgcggt tttggcgagg atgtcgctgg
    34621 tgagcaggat gccctgaccg cccacgccaa ctaaggaaac gtttgtgacg ttctcactca
    34681 tgctcttctc cttcaggggg ggtacaagag cttctttcga ttcgcccgcg cgcgaccccc
    34741 acgtaatcaa cttgaacctt tttgaacaga tgaacatcct gattgcgctc aataccaaca
    34801 taactccgcc cttccaacgc agcagcaaca agaaaactcc cgctcccaaa agcgttgtca
    34861 agcaccagtg ctctaggctc ggtaaacgtc cggattaaat accgcccgag ttcaacaggt
    34921 ttctgcgttg gatgccacac cggcccctcg ctctcggctg ttttgaaata gacgacatct
    34981 gtaggatagc gcgcaccact actctttaca cgcgccggtt ggaaatcacc gtagctaccg
    35041 gacaattggt ttttccgcac acctttatca tacggctctc cactattcat ctgtgggttg
    35101 taaacgggtt gcctagcata aaaaacgcac acatcctcat gctttctgag cggttgcctt
    35161 ttagcgttca agaaatttgt gggtttggat ttttcccaaa cgattttgta tttgaaccat
    35221 gcctcgttgc tcaaaataac tctggcggtg aaaatcccct gcgcggttag aaccacgaca
    35281 cctctcggct taagaatcct gcggtactgc tcccaaagtg gttccaaagg gataagactg
    35341 tcccatttgt tttgagttgt cccgtaaggc agatcgcaca gcaccaagtc tacgcaggca
    35401 tcgggaaaaa acggcaatat ctccagacaa tcgccttcga aaagcttgtt cttgatatca
    35461 cgaggcttac gcttgctctt cggaacaaaa catcgtccgt cgttaggttt gaatgtcttt
    35521 tttttaccca taagaagaat gtgaccggct accgcacccg tttgatcgcg tcgaatttgc
    35581 agacctgcgg gcacaggtca cacccagagc actgcaccgg atcgatgcgc gccttgtagc
    35641 gcttcccttc aacaaccgtt tcgccccttt caatcgcagg gcagcccagc ttgaaacatg
    35701 cgccgcaagc cttgcacttc tccgcatcca cctcgaaagt gccggcgttc atcagcttgg
    35761 ccgcgatgcg gcaaggtccg ccagccacca ccactgaagg ctcatccgcg tccaactctt
    35821 ccgccaacac cttttccagt gtcttgagat cgtatgcgtc gaccttcaca acgcgccgga
    35881 accccatggc cttggcaacc tccgctatat ccgtgaccgg cgcgggatca ccgcacaggg
    35941 tcttgcccga ccccggattg tcctgatgcc cggtcatgcc ggtgatccgg ttgtcgagca
    36001 cgaccgtcgt ggtcctgccg ccgttgtaca gcacattcaa gataccggtc atgccggaat
    36061 ggaaaaaggt ggagtcaccg atcaccgcgc agaggcgtcc gcccttgccg gctttctcca
    36121 taccgtaggc gttgccgacg gaggcgccca tgcagatcgt ggtgtccatg gcgttgagcg
    36181 gggggaacgc ccccagggtg tagcatccga tgtcgcccgt caccgtggcg ccgagcttgg
    36241 cgagcgtgta aaacaccccg cggtggctgc acccggcgca cagaaccggc ggccgcgtgg
    36301 ggagtccggc atccgcccgc cggacttccg gcagcgtgat gtcgccgagc aattcgttac
    36361 gcagcgtcat gagccgcagc ggattcatct cgaacatgtt caactcggtt gtgtgcccca
    36421 cgaccgggat gcccaacgcc cggacctgct cctcgatgaa cgggtccagc tcttcgatca
    36481 ccagtacgcg ctccacgctt gccgcgaact cccggacccg ttcggctggg aacggatttg
    36541 aaaagcccag tttcagcacg gcgaattcgg ggaagacctc tttcacgtac tggtaggtca
    36601 ccccggaggt gatgatcccg aaacgcgtgt cgcctgcctc aatccggttg aacggcgacg
    36661 cgcaggccag atcacgcagg accgcagtac gctcctgcgc cttcaggcgt tgcggccgcg
    36721 cgaacatcgg taccgggatg tagcgccgga tatctttgac atacggtttg cgtggcggat
    36781 ggccgggggc gaagtcatcc cccacatcca ccacggtgga ggtgtgcgaa gtccgcgtgg
    36841 tcagacgtac gaacaccggc acctgatagc gctccgagac ctcgaacgcc tcgattacca
    36901 tgtcgaaggc ctcttggctg tccgcaggct cgaacaatac ggcgcgcgcg aagcgcacca
    36961 gagtgcgatt gtcctgctcg ttctgcgaac tgtgcatccc gggatcgtca gccgtgatga
    37021 tcaccagtcc gccgtttgcc cccacatacg tgtaggtcat gagcggatcc atcgccacgt
    37081 tcaggccgac gtgcttcatc gcggtcaacg cgcgcgcgcc ggagatcgac gcgccgatcg
    37141 cgacctccat gccgaccttc tcgtttaccg cccattcgca gtagatcgac gctttgaatt
    37201 tggcgacgtt ctccagaatc tccgtacttg gcgtgcccgg ataagcggac gccacagcgc
    37261 accccgcttt gcaggccgcc cacgccaccg cttcattcgc cgacagcagg actctctgac
    37321 tcattgtttg tctccattcc ccgatagggg tagcgcttcg cgcgggaggc ccggaacggg
    37381 cgcactgccc cataaaataa aaaaaacctg cttcctccgg ttttccgaat acatcctgat
    37441 ctggcgggag atcgtggact cgaaccaccc accccttacc atttgaacca attacatgta
    37501 aaatgtagca tattgtttca gtactgggaa gggtgagtta tggacgcgga agctatggtg
    37561 acttgcgcgg cattcaccgg atcacgctgc ccgaggacgc ttaccggacc tccatgatca
    37621 gcccccggat ctgatacggc gcgtagcagc ccatcgggac atcgcccggt tcggacggca
    37681 tggggacgcc gttcaggtac ttgaggcccg gcatccagtc ctgccacaca cccgcctttg
    37741 cacatggcga atcgctggcg atgcagtaaa ggccgcggct caggctgctg cggaactgtg
    37801 gatcgctatc ccgattgcct tcgcccgtcg gcttcggact cgagcaggaa tatgtcatcg
    37861 ccgccgtatc gtagttcttc gcgccgttgt tccagacgat gcagttgagg agcgttgcgt
    37921 caccgctgat gccgtccgta ccgtccgtga gttgcgaatc gttgccgacg actgtgcagt
    37981 tgtagagcct tgccgcgccg ctgacggctc caccctgtcc cgcctccgac tcgttgtcgg
    38041 cgatcaggca gttatagaga ttaccgacgc cgtacacgcc accgccttcg agataagcgt
    38101 agttgtcgcg aatgacggta ttgtagaccg agcccgccgc cgcgaacacg atgccgccgc
    38161 ccgtgcaaac ggttttcgag ccaccttgcg gaaggttcgc gcggttcgcc gcgatgacgc
    38221 agttcgagac caccgcgccc gcattgcgga tccacacgcc accgccgctt cgaggaccac
    38281 cccatgtggt ttccgtatgt ttgtgggcag cgcagctctg gttgttggtg atgatcgagt
    38341 cgaagagacg cgccttcgcg ccgctcaagc cgaccggtcc gttctggcga ccgttgttgt
    38401 tgatgatctc gcagcgccgc gcaaggccgt tcacaatcgt cagcgccaca ccgcctttgt
    38461 cataaccgtt cacgctgccg aagccgctga acagcgaatc ctcggcaacc gcgtcttccg
    38521 tgttgaggta gacgccgcat cccgccgcta tgtcgacaaa cgcgtcacgg aagacgcagt
    38581 cgcttagcag acccgcgttg atataggccg tcgagccgtc gatgttgccc gagcggttgt
    38641 atttgttgtc cgggccggcg aagtcgatgt gctggacctt cgcatctttg ttgtcgacgt
    38701 agaggagacg cttgccgccg aagcccgtcc aattttctgt gctggcttta taggacacct
    38761 tgacgtccgt acggttggct cccgtaccga ccagcgcaat ggcgttggtg ataacaagcg
    38821 cctgatggtc agggtcaaag ccataggtgg ccggcgcgac gtaaacagtg tcaccgtcat
    38881 ccgccaccgc gagcgcctga tggattgtct tgaacggcgc tgtagacgtg ccggggttgc
    38941 catcatcgcc cgtcacgcca tcgacatgcc agtcacggct gaaaaccgcc gtgaccgtgc
    39001 gcgcgaaatc ggacacaagc gtcagcgtgg ggctggtcgg atcgttgtca ccgatggcac
    39061 cttcccagcg ccagaaacgg tagcccgccg ccggggtagc cgtcgccgtg aacgtcgcgc
    39121 cgcgcgtgac gaacccggat cccgtcacca gtcccgcgcc gttgccacga ttcgttaccg
    39181 tctgcttgta ctccgccaga aggtgccatg tcagggtgcc gaggcgtgcg tacgcggccg
    39241 catacgcggt gtccgcaccc tcgtttgtcg ttacgttgtt ggcgttgtcc tgcgccacgt
    39301 aggtatagcc cgtgcaaaca acgttcgtga gacccgtcgc atccgtatat ggcgagggaa
    39361 ccgaggcgtt caccgcgcca accgttgtat cgggatgttc accgaatgcg ggtgtcgccg
    39421 gtacgccgaa ggtctcggga tcacccacga cgcggaagtt cggcgccgcc tccagcgcac
    39481 cgtagtcgag cgcgccgttc gccacacgcg gacggcccgc ggcgtccgtt tccggcagca
    39541 tcaccgacgg gacgcttgcc gtgtcgatgc agggcgaacc gatgtcgagg cgatagtctc
    39601 ccgccgccgc gtcgaggaac ttcgggtctt ccgtatacgt cgccgtcagt gtcacgttcg
    39661 cagaatcaag cgtgccgttg ccccagacaa tgccgttctc gaccgttgcc ttgttgttcg
    39721 catccgagcc tgtcacgcca tccacaccgc catcgagcag cgagtcgttc cctgtcaccg
    39781 tgcagttgta gagaaacgcc ttgccgtaga ctccgccgcc tgtgcggaac tgcgccacgt
    39841 tgtcggcgat cacacagttg tagagatagc ccgagcccca cacgccgccg ccctcgctcg
    39901 cggacgtatt gccgatgacg actgtgttgt agacctttcc cgccgaggcg aaccagatgc
    39961 cgcccgcccg atagtggttg tcggagcctg tcgtcatgcg ttcgcccgat atgttggccg
    40021 aaacgacaca gttcgagaca accgcattcg cgttgcggat catgataccg cccgcgccag
    40081 agcccggcca gggcgtcagg ccgttgacgc ggtcatagcc gcatccgaca tttccaatga
    40141 cgctcgagtc gaggagcttc gcattggcgc ccgaaagcaa cacgccgccc gtgttttggc
    40201 gcgagacgtt gtcgtgaata tagcaaccgc gcgccgtacc gttgtggagc gtcaggcctg
    40261 ccgcacaaaa tattccgccc tgattactgc cagcgccgtc cacattgaag tggccgtaga
    40321 tctcgcaatc gacgacatac gcatcctcgg tctgtgcata aacgccgccg taacccttgt
    40381 tgacgcgcca cgagtcggca aaaacacagt tcgtcaccgt acccgcataa atccacgcgc
    40441 agttcgcggg gtagatgccg tccgagtatc ggccgtaacc gccgcggaac ttgatgttcg
    40501 ccactttcgc aagcgcgttg ctcaccacaa gcggacggcc gctgaaggac gaattctccc
    40561 gacccgaaag cacataggtg tcgctcgctt ccgcgcccgt accgacgatc tcgacggcct
    40621 tgtccacgag gagcgacgca gtgatcttgt aggtcgccgg ggcgacttcg atgcggtcat
    40681 tgtcgatggc gacggtcagt gccttgccga tcgtcttgaa cggcgcagcc gaagtaccgt
    40741 cgttggcgtc gtttccgctc gtgccattga cgtaatatgt cgtagcggcg tgtgagaacg
    40801 aaacgaacgt agccaacgcc gcccccatta cgaacttcat cgtcgtcata cccatagcct
    40861 gagtcctttc tgcaaacatc aactcatccg gcttgctcgc ataggttcta tcgaaacact
    40921 ttactttact tttatatcaa acaaccctgt tcgccgtcaa ctatcgccaa atcaggacat
    40981 cgaaacgacg gtcaccggcc ttctgcggta cgcctcatgt cgagtcggca acatggggcg
    41041 cccccgcgtc atacccgatc ttcaacacgc gggcacagcc ctccggcaga aagtcgcgcg
    41101 catggcgtcc gccggtttat tcctgcgcct tctttgcggg ataatgcacg accgagacgt
    41161 tgcccaccgc gtcaaccgcg aagaccgcgt acttgtacgc cgcctcgatc tggaggcggt
    41221 gcgtccacgt cttcgcgccg ttgttcagca cgcccgaatc gtagaccgtc cggaagttgt
    41281 ggtccacgac cttgacctga cggatcgccg cctgcgactc cgcgcagatc gtaagttccg
    41341 agtacgcctc cacgagttcg atgttgtcca cgtaacccga accgccggaa taggcgagac
    41401 cgaccgtcat ctggccgtgg atgatccagt cgggcatggt gaccggctgc gagtacgtgc
    41461 gaaaccatct gtcgggaccg tcggtatatg cgcacagact gaccgcgcgc ttccagtcct
    41521 cgctccagta ggtcagtccg cagccgaggc cgctgccgcc gttcgagaag gtgtgggtgt
    41581 tgacctttga cgacggtttg atctcgcacg agaggatgta ggtgcgcccc ggtttgacca
    41641 ccgaggtcgg tatgcttctc cagacggatt tcttgtcctt cgtgaagaac gacaatcccc
    41701 gcccttccat ctcgatgcaa cagacctcgt tggagtggct gttcgcccag cccttgagtc
    41761 cctcgtcgaa acggaagaat ccgttcgtaa ggatattggg cgcaggcagc tcgggatcgg
    41821 ccgagagcgt gacggtcagc gcgttcgcgc taagcacgaa cgcgaaggaa gaggcaagat
    41881 gcaggaagta aaaaggtctg attcttgtct tcattttcgt ttctcacagc tcctcgacgg
    41941 aaataccgta gccttcgagc gtcatcttct tgtcgcaaaa gcccggaatc tcgaccggtg
    42001 cggcggcgac gggattgttc tcgatgttga cgacagagag aaccttgcgt ccctcgaact
    42061 cgaccgcgag cacgtccagg acaccctgtc ggccctgaaa cttcacttcg ccctggcaga
    42121 gcgctggcgt ccacttctgc aggaacggag agaacgcttt gaacatgctc atcccctccg
    42181 gatgctggcg gacaccgttg cgctgcgaca gcggcgccca cgtgaagaag gcgaggccct
    42241 tgacgccgtg attgatcgcc agcatcgaca tgcacttcac ctcggccggc gtcggcggac
    42301 gcgtccagcc gccctcgcct ccgaagttct ggatccagag ccagcgaccg acctgtggac
    42361 ggcccttcgt gttggcgacg agacggtcgg cgtgtgccgc aaccgccgtc acacgtccat
    42421 tcggaatcgg atagggatcg gtcgagagga tgtcggcggc atccttgttc gccgaaacgg
    42481 cattgatgac gttcagccac gtcagacgcg tcgggtcgag ttcgtggtag agccggttca
    42541 ggagtgtatt gtactcgacc tgtccatccg tctcgtcggc cgtatgccac aacatcacgt
    42601 tcggcgtcct gcggatgcgc gcgacatgtt cgacgaagca atccgtcgcg tagggcgact
    42661 tcggccgata aggcgaagtg gcgatgacgt tgatgccgta cttcttggcc atcctgcact
    42721 ggtcctcgaa cgtaacaccc gcatcgtgca gtgacttcgt gatcttctga ttccaggcgg
    42781 cgatcgactt cgtcttgcgg cggacgtcga gatcctcctc gggaaagctg taatctgtcg
    42841 tcatgcccgt cgtaaaaccg tgttccgcca tctccttgag cactgcttcg gattgcgacc
    42901 gccattgctc aagcgatgcg aagtccgacg acgtcagctt ccacgtgatg atcgggaaga
    42961 agaaccggcc gttgcgatag acgcagtggt tcctgatcgt ataacggtcg acgttgtcgg
    43021 aatcccgcat cggcggaacc ttgtagaacg tcttctcgcc cgacttgatg gtcttgcccg
    43081 ccgcatcgac gatcgcgatc gtcgccacat gcttgccgtc cgcgaaacct ttgagtggga
    43141 acgcgaaatc agcgtggaac gccgtcaacg gcgcattgtg ctcgaggacg gtcttgccgg
    43201 tggcatcaac gatggtcgcc ttcgtggtga agcccgcgat agacttcgtg tagaggaagt
    43261 cgagcgttgc cttcacgtcc gcctggtcgg tatagacgac gcggtcgaat acgatctcga
    43321 agtccttgcg cgcggcgtct ttctgcgcct tcatgatcgc cacgaggtcc gcgacgatgc
    43381 cgtttacact gttgaatgag gcgggttcac cgaagacacc gttctcgagc tttgaccaac
    43441 tggacgactc gcccttcttg tagttcccgc gcgcgacgtt gatgcgccag ttggcgttat
    43501 agctctgcgc gtccagcttg atcgccgcaa acgggatctc ggcctccatc atccagtacg
    43561 tctggtagag ctgcaccgag cagcggaacg gagcgaccat gccgacgctc gtcacgttgc
    43621 ctgcaacgtt gatggcgatg tgcgcgtagt tgcccgtgtc acccgggtcg aggaacagct
    43681 cgatgcagtc gcccgcgaag ccgctcttcg acttgtcgtt gacgatcttc tcccggagcg
    43741 cgtagtcggt gaacgagcac ttcgcgccga tgtaaagcgt gtcagccgta cagaagaggt
    43801 atgcgcgcgt ctcctccgtg atcttgtcgc ccggcttgat ggccgtaaag ggtgcgctcg
    43861 cgtaggcctt cttccagcaa ggatcagaca ggtcgccgtc cacgatgttc ggcggggaaa
    43921 gcgtcgggat catcaccgtc tcataggcga acgcatgccc cgccagcacc atcgccagcg
    43981 ccagcgcgcc ggacgtcatc gtcttcttgt gcatcgtctc cctttcatgt cctgattgat
    44041 tctctatatc ttacactacc ctgcaccgct cctcaacccc ttgtgttcgt gaggtcgatg
    44101 gggcgaaaag caaaaaataa aaaccccgtt ttcattgggc ttttccaata aaaacggggc
    44161 tgaaatctgg tgggagatag tggactcgaa ccaccgaccc cttgcatgtc aagcaagtgc
    44221 tctaaccaac tgagctaatc ccccgaagcg gaaaaaacgg aagtagtgta aacgatgcga
    44281 tgttccctgt caaccgccgg gaacccggct ttcgcatttc ctccctgtcg gccagacgga
    44341 aaaccgttcc gggaaacacg ggaatatgat attacatgga acaaacggag tccgggcctg
    44401 cggaacatgt gccggtatgc ggtagcgggg gggggggaca acgcgttcgg gagagagggg
    44461 aaaaagatga acaggggtct tgtggtcggg atgatatcac tcacgctcat gggcgtccgc
    44521 gccgagacgt gggtgcctga aaaaatggcg gagtcgcttc acatggggcg caggctcgag
    44581 gttttcacga atgaggtccg ggcagcgtgg ggttatgcgg caccgcaaaa agacgcgttc
    44641 ctcgtactgc atcccaagca ggagcgcaag tgtgcgccgc tctatgtggt gctgcactcg
    44701 gctggtcatg acgccttctc ttgtctggcc tgcacacgca cgcccggcaa ccacgacatc
    44761 taccatgctc ccgaagagtt tttcgcgttg tacctcgact gccgcggcaa caagggggac
    44821 tggtggtggg gtatccagcg aaccaaagga ccggatatcg gccccacgga aaagcgtgtg
    44881 atcgacacgg tgaagtgggt gatcgagcag tatgcggttg atccggaacg ggtctacctg
    44941 tgcggtaact caatgggcgg cagcggcgca ctcggcatcg gtatgcgcca cggcgagatt
    45001 ttcgccgcga tcaaggcgaa cgtgcccgca cgcgtcgagc acgtctccag ccgcatgggg
    45061 tttccgccgt acgcgccgtt acctgcgggc acacgcctcg ccgacccgcc gatctgtatc
    45121 gactactcgg cccccaacga cacttggtcc accaaccatg agacgtttgt cagtgcgatg
    45181 aatctccgca agtacccgct ttatttctac tggggcccct tcggacatgc gaacaacgac
    45241 gggaagattc tggcggtgaa cgacctcgtg cactcctttg actggctgaa cgtgcggaaa
    45301 aatgagctgt acgctgtctt tacggacgcc tcgaccaacg atccgctgcc gtggcccgcg
    45361 cggcttgatg acaaaaaatc tggacagatc aacgcttttt tccgatggaa accgttggcc
    45421 gaccgtccgc gtcgcgtgga ggtttcgctg tttctgctga caccccagat atgcaaaacc
    45481 tcgttcacgc tgcccacgga ggcgacggca aacgtgacgc tgcgacgctt acagcgtttc
    45541 accgtcaagc cgggagcgga agtccgctgg tcgttcggcg aacgggaggg cacggttcgc
    45601 gccgactctg agggactcat cacgatcccg ggtctgaaga tcaccggtga accgcagacg
    45661 ctgcgtctgg tcaagcccgg ctggtggcgt tgagaaggag ctgatttcac ctgtctttcc
    45721 acaacccgcg actcaacgat ttccgaatac gtcaggagac ccctcaacgg tgctttgaat
    45781 tgcaggaatc atgaccatgc cgccacgtct tacgaaaaat aaaaacaaac cgcccatctc
    45841 cgccattccg tttgaaaaat gtgcggccaa gacgacaacg gacggcaagc cgggcacgac
    45901 ggtggtggag cactgcatcc atgtcgggcg tgttgcggaa gctctctccg cgctgaaacc
    45961 tgagtacgtc aaacggctct tgccccctcg tcctgggttg cctaccggtg tgcatgatgt
    46021 cggcaaagtc tcgccgggct atgcattgaa atatttcgcc aatactgtcg tccgtgaaca
    46081 cgcgccggaa ctatgtaacc aaagctcttt ctgcaagctt cacgccacga tcagtgccgc
    46141 cgcgatcgaa cgctggctga acacgcccgt cacgcgtcac tcacccgttg cgctggcagc
    46201 ggcggcgcat cacggcaaga tggaaacacc gccctttcct cccgacatgt gtgaaatact
    46261 gggcggatcc gcatggtcag aagagcggca aaagctgatc gcgcgtctct ctgaggagtt
    46321 tgatggcacc ctgcaagatg ctgccgaagc caatgaatgg ttgctggccg gcctgacatg
    46381 cgtcgcggac tggatcggct ccgatgagac gttctttccc gccgaccgtc tgccgattgt
    46441 ggacggagat gccgtcctga cggcgcgtca tgcggtcaag gagtgcggtt t
//
LOCUS       DAOSLE010000005        46044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_935_length_46044_cov_9.086368, whole genome shotgun sequence.
ACCESSION   DAOSLE010000005 DAOSLE010000000
VERSION     DAOSLE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 46044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46044
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_46044_cov_9.086368"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            344..712
                     /locus_tag="PK576_00700"
     CDS             344..712
                     /locus_tag="PK576_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60140.1"
                     /translation="MKHMKGNWLMNRCAVACGCLALVQATFAGSATWNYREGAGGSPA
                     NPTLWTDTANWTGGAVRLTGDTSKFTTGSYKLIASSGLIPGSEWTVDGYTGRKVLKLR
                     VKADGLYLEVIEQGLSVVIR"
     gene            752..1993
                     /locus_tag="PK576_00705"
     CDS             752..1993
                     /locus_tag="PK576_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60141.1"
                     /translation="MKKECFSLCLTVVAAVVVGLDARAANIEFYRRSKTGGSWNDVNH
                     WVTNAGGSVLMGTYPKDPATDRVWFKDGFTNDTQELYLESDINVLNMMQLNASAGNQT
                     NYLNLGGHTLYVVNFLNQDYGCVGTFAITNGNISSMMIRTIGSGYCLWFKGPNTRFDL
                     HGDWYRICIGYNSARSTLRVSDGAVCNWPCTMFYLGGEQLSGKHDGNRLVIDSGGLVT
                     NCNTDLVINRGGGTNGLYVSDGGKFHSNAGIQMNSSDGGWHTNPVMKSVTAILDVRDG
                     TVDIRRLTGSVGSNSVLRLAGPQSDLRIRNEMVITNTFDMTFDMTDGVFAAETAPIRL
                     TSTSHTHILGDGTIRVRYPQRFAKEHKGLIPLIHAASGNLTYTGDIRFDFTEARWAQA
                     VTNSTMVAIKIVPPGLVFSIQ"
     gene            2049..3563
                     /locus_tag="PK576_00710"
     CDS             2049..3563
                     /locus_tag="PK576_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60142.1"
                     /translation="MKTVWKTSRGWGAIALSAWVLCVRGAIVDPADYGFSPDAPAATN
                     AVALQRALDGGKRTVQVTRPGVYGLDRTVWLDSDTRLIFTPGAVLQKQAAYVHVLANR
                     GLESWAWDTNIVVEGATIDVNDFSAGHGEDSPQYGNRGQISFCRIRDLTVRKFTCLNL
                     HGGQYGFQIATFENVILDEFEIRGAKDGVHVNAGRKFVIRNGILETGDDGIALTAMDW
                     VSSAPVVGDIEDGLIENITDDGRGRCNFSRILTGAWTDWHKGIRLQRGDTVRCGKKLY
                     RVAMPRGTNEYVSLEAPTHTRGIWTDATGLKFWFMQDSDALAANIRNVTFRNLYLRSP
                     RNGFLADWETRSSWNRGIHPEVKPENYPVCEVRLEDVYSESPGALVSGYESVRLWLRN
                     ITKTRGPLVNLARPNDNRDCAIDVWLHLSGVVLKADDGKGADISVGGAGSSLNLTVEG
                     LMQERDLRVSAGAGVRARVNGIGSIHSLEGLTPEAGDSIKVGGVLKTFDGTQWR"
     gene            complement(3610..4692)
                     /locus_tag="PK576_00715"
     CDS             complement(3610..4692)
                     /locus_tag="PK576_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60143.1"
                     /translation="MKRKRVLHIALIYNTYRDAVPEAPDDRGSTNDLRRFIRSLARAL
                     RSLGHRVTVLPLANDMLGFQRKLARLQPDVVFNQYDDVVHSALYEMRVAALVRMMGFP
                     ITGSHALALGLCRYKHMAASLLQGAGLPVPPDARLLERVKDVSGVKWRFPLIVQAAKE
                     HAGVGLTRHSVVHSVTALRRQVGIILRDYHQPATVQRFLPGREFNIGIIGGAKQRVLP
                     FAEVNYAGLPPGIPPIMSYDAKWTESSIEYKCTSVICPADVAPPLARKLTNLTVKAFR
                     ALGGWGYGRVDIRLDEAGEPHVLEVNCNPCLEEDVALARSAKAAGIAYPQLLQQILDA
                     AFEKPPFDTDVPMLPPPRPQNPLRPT"
     gene            complement(4801..5790)
                     /locus_tag="PK576_00720"
     CDS             complement(4801..5790)
                     /locus_tag="PK576_00720"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOE60144.1"
                     /translation="MHITIIGDGGWGTAVGLLLNGYGHAVTLWGPFPEVVDEIRERRE
                     NTRFLPGIPLPETLRWTADAAEAVAGADVVILASPSKFLAQVCERFKGLIPADCLTVS
                     LTKGLCETTHCRMSQLARTILGLRSIVVLSGPSHAEEVARRLPTAVVAASEEEELTRR
                     VQQLFSGPAFRVYTSPDPLGVEIGGAVKNVIAIAVGVSDGLGFGDNSRAALITRGLAE
                     ITRFGTALGACPATFSGLSGAGDLIVTCTSRHSRNHMVGERLGRGERIQEILDSMKMV
                     AEGVWNAKIIHALSRTHGVSMPITEVVHALCYEGLSARDAVEALMTREVKPEQ"
     gene            complement(5817..6476)
                     /gene="plsY"
                     /locus_tag="PK576_00725"
     CDS             complement(5817..6476)
                     /gene="plsY"
                     /locus_tag="PK576_00725"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOE60145.1"
                     /translation="MTTVTVWIVGGVAAYLLGSIPFGFLLARSRGKDIRTLGSGNIGA
                     TNVFRSVSKPLGVLTFALDFLKGLGGVTLIPALALTICPPETGAAGGKGLAMFCGALT
                     VVGHNWTCFLRFKGGKGVATSTGLVLGLAPQAVGIAFVVWLVVFLMTRMVSAASVAAA
                     VTLAAAVWWPLRLHAAYGFWFPAVFTALAAVAVWKHRSNLARIRNGTEARFTFRTRRQ
                     V"
     gene            6659..8116
                     /locus_tag="PK576_00730"
     CDS             6659..8116
                     /locus_tag="PK576_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60146.1"
                     /translation="MKSMLRWFIGIGLAGVLFAEEKSPAGQGAGAAAPADVAQMKARE
                     TGGLDWDAGADLRVRQEASDNLPKRINAQGEGVTAKNDNYLRIRPRVWGQVKNEDFKL
                     YLRVADEFREYFQPRESRNARPPDEVLVDSLYLDLYNLADGWLDLRIGRQDFFGPDGP
                     VYGAGRVICDGTPFDGSRTIFMDAIKATVKFDEKNSLDLLAIYNSAETEMSWGHPYRT
                     DGSEYKERPLTSIHPASKDMDEYGGGLYFRSRSVPEVPFELYAFYKRETKARLAGGAR
                     LPGRNTYTWGLRVMPQLTETLSAEVEGAVQAGEKDGGASTSGYMGYAGLTYRPLIDGS
                     SKPFFTGSLYYLSGDKNRDAEGDNDTAWNPLWARWPQFSEMYVYSFMYGVGYWSNLLY
                     PSLEAGVGFAPGHKVRASVGPMYVAVEDDLGGGNGDLLGWLGVVRYDFPLFKNIFGKR
                     GDLTGHVTAEVLDPGDYYVSDTVAYFLRWEVIARF"
     gene            8193..8705
                     /locus_tag="PK576_00735"
     CDS             8193..8705
                     /locus_tag="PK576_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60147.1"
                     /translation="MMWVVLAPLLLQARVGENLKQSETRYGTGEELQDAETFILKNAR
                     NVVYFHGDWIITAGYLNNVTARIQYEKAGLRQPRAQLTREDVAEILEAEDAAGWSAYN
                     EETRQAGKAYTGKTHPPPVLRSVSGLTARYKVFSVTVEREEASHHEKGTTLRQPLRPT
                     LTPRGKRVAL"
     gene            complement(8733..9953)
                     /locus_tag="PK576_00740"
     CDS             complement(8733..9953)
                     /locus_tag="PK576_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOE60148.1"
                     /translation="MLIKPFAALRPAPDRAASLASVPYDVVDTAEARALAAGNPDSFL
                     HVSRPEIDLPDTVDIYDDAVYAQGARAFAELQARGVLLREAEERIYVYRQIMGTHSQT
                     GVVACCHIDDYARDVIRKHEKTRQDKEDDRTRHCLELNANSGPVFLTYRTREDLDARV
                     AQAQQATPLYDFTAADGVRHTVWRAEDDPAAWVEAFHHVPLAYVADGHHRAAAAFRAG
                     KQRREAHPGHTGHEEYNWFLAVLFPADQLRILPYNRCVADLNGLTPDAFLERVRTLFR
                     TEPTDGAEPAGPREIRMFLNGAWHRLTWEAVDADPVGRLDVSVLQNRLLGPVLGIDDP
                     RTNTRIAFVGGIRGTGELVQRVTSGQDAVAFSMFPVTVQQMMDIADAGQIMPPKSTWF
                     EPKLRSGLLVHTLD"
     gene            complement(10038..10547)
                     /locus_tag="PK576_00745"
     CDS             complement(10038..10547)
                     /locus_tag="PK576_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60149.1"
                     /translation="MKRMWSGLVLACLIAVGAQAAEKVRWPVWCAFNDTEDIDVVGLR
                     LNLPSGQCEQVTGLDIGLIGRSQYFNGVQINLLRNSVLDALAGWQIGCYNSTGLGDML
                     GMQTGLWNESRTLYGLQVGLINLADYAEGFQVGLVNRAEDMHGYQIGLINIIRGGDVP
                     FCPLINIGF"
     gene            10765..12279
                     /locus_tag="PK576_00750"
     CDS             10765..12279
                     /locus_tag="PK576_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010599709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE60150.1"
                     /translation="MNVWIRTAGVGVFWAGLLWAGDPVPYGAIPSERQVRWHEIEVFS
                     LIHYGLNTYTDREWGYGEEDPALFNPANLDANQIVSALRSGGMRGVILVAKHHDGFCL
                     WPTQTTAHNISKSPWRGGKGDLVREVADAAKKHGLHFGVYCSPWDRNHAAYGTPAYLA
                     VYQAQLRELLSQYGTLFEVWFDGANGGDGYYGGAREKRSIDRFTYYDWPNTWKIVREL
                     QPSACIFGDVGPDCRWVGNEKGYAAEESWATFTPKGKTDPARPANGDSMNHIEGPAGH
                     RNGTAWMPAECDVPLRKGWFWHPDQDATVRSPATLLDIYFRSVGKGGCMNLGVAPDRN
                     GRVCEADTRSLEAFGNLLSVLFAVNFAEQATVSASHVRAWEPAPGKTHPYEPARVVDR
                     DRYSYWATDDGVTQATLTLTLPAAVEFDVVRLRENIRLGHRVDGFVVEVWQGQAWKEY
                     AKGQSIGACRLIRGSKVQTDRVRLRITSAAACPCISDFGLFALPATLAPSLPKP"
     gene            12276..13001
                     /gene="rph"
                     /locus_tag="PK576_00755"
     CDS             12276..13001
                     /gene="rph"
                     /locus_tag="PK576_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOE60151.1"
                     /translation="MKKTPARLRPVSFERRFTRHAAGSVLIRQGDTWVLCTASVEEKV
                     PPFLRGSGKGWVTAEYSMLPGSTIERKEREIRRGKPDARGTEISRLIGRALRAAVDLE
                     RLGERTITIDCDVLQADGGTRCASVTGGMVALADALTALVRQGKLSSPPLRHFVAAVS
                     VGICGGKPVLDLDYAHDSVAEVDLNVVMTDDGRYVEVQGTAEGEPFDEAVLDTLRSRA
                     RKGIRDLIRMQKAALREEPPHHP"
     gene            13091..15193
                     /locus_tag="PK576_00760"
     CDS             13091..15193
                     /locus_tag="PK576_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE60152.1"
                     /translation="MLFRTILFCCAFALLAVCRGQTNTPVDLGTFVVEGTPISKYRAE
                     TVSTATFADAKPEELPQTVEVLTEDFISEMNPVDLHDMLRHQAGVYTGGKSMLDRTSG
                     QYTLRGMSGSDVMLNGTLGLAGPMGTFMDPSAFERIEVVKGPVGSTVGGASSTLGPYG
                     AGGSVNLVMKQPRDDQDFLHLKPRASLGEHSQRYRLPFDINERVIEDRLTVRLPGNIE
                     YGRPFWLSSGYDWRESYYLAPSVFMAVRDDLRIGANLSIQYTDQPAYQGIPVYKGKPY
                     AGYDWDSNPALSHMRDTYIGHTLDAYVEWDASRVWTLRSGAGVAQADIEFEHLGPAAF
                     ANASTDALLPLPLAKPYDHQEGDMLYRRYNAYQRATAVFDTGPVGHQAVFQGDFSRKT
                     SKGRSYFESVAKPDAVHRWVAADYQNTELDKYGFLAQDYLSWWKLRVLVGGRFDDHES
                     DQGNSANAFCPRGGISFLPTDWLVFFANVSYTEAPNFGYNGKDNRELTSTWEAIQYET
                     GFRVAPVKTLWLSAALYRIDQDNTPIALPEDRTRYTEEGANESQGVEVSLTGNISDRW
                     SVYASYAYNEYENKQQKKRFDRYPPHAATLSTSYRLAGGPLDDIVLGFGYRYRHRYAA
                     TMRGEYVGDNFYIDDSHVFDCSADIPLYKFGGPKNVTLSLACKNIFNRHYIESTRHYY
                     QCFPGDPRTFELALQAEF"
     gene            complement(15322..16677)
                     /locus_tag="PK576_00765"
     CDS             complement(15322..16677)
                     /locus_tag="PK576_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE60153.1"
                     /translation="MRVRGITRRKFLSGGTGAGAALICGRTFAEGEAAGLAELIRALG
                     WDPNAPGSFFFLQATDLHASEITSGALKMPDKFGGRNFADDMNALRPAPAFVALTGDL
                     IAATTRNPATWPQAEAGFRRVRDLIVSRLTVPCHMIIGNNDCSPEAYHAVWPDRPLYW
                     SFDRQGVHFVGLHGYNTWKPENTNHAGILLDDSQLVWLKRDLEAAANAKTLVLFTHEA
                     LKDPDCHRIRKQLAPLLAAFPGEIWNIAGHNHGNAECEIRLGGKPVRVVETITPVGAW
                     TPDKGAYRLVFVSNGRVVGSGLRWLTKEGVPLRFEAVASDRNIPTRTLIETALAEGAH
                     RVFMVGEEDLPLRLDTTGVTDRISNFQFRKGAALTYRLPLTLRGKDATRLRLACGAKV
                     SVTFSADGTVWHPASKQAHAAGVVTVDVPEALRATANLTVRIQAPPDQGARLYGIAVL
                     R"
     gene            16920..17399
                     /gene="dtd"
                     /locus_tag="PK576_00770"
     CDS             16920..17399
                     /gene="dtd"
                     /locus_tag="PK576_00770"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE60154.1"
                     /translation="MKALIQRVAEASVEVEGRRVAAIGRGLLVLLGVTHGDTLKEVAW
                     LARKIAALRIFEDEQGLMNRSVSETGGAVIVVSQFTLYADTRKGNRPSFTEAARPEQA
                     EPLYRELVRQLRLTLGADRVGTGVFGAEMKVRLLNDGPVTVELCTEVACPPPRTSCG"
     gene            complement(17476..18402)
                     /locus_tag="PK576_00775"
     CDS             complement(17476..18402)
                     /locus_tag="PK576_00775"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE60155.1"
                     /translation="MTSPTNPCRMSLLKKFAAYLEYLAIRFLGMFYGLVPASRAYDLG
                     ARLTALAYPLFAARRRTAVENILKAHITDDPDEADRIARQAFGHLAGHLCESLKAGNV
                     ISDETWRDHITLDGPAESFALLLESPGVPILILTGHLGAWESAVPILSRARPMIAVAR
                     TLNNPFVERFLKRHHFRGQITIIPKKKGFSPDVLRAWKNQGAAMTLVMDQHAGPKQGV
                     TVDFLGRPASTHTSPARLHLQTGAPILVGGFMREGPFRYRMVTGLPIRFQPTENREAD
                     VRALTAEINARLGEVIRRYPEQYLWAHKRWRV"
     gene            complement(18386..21769)
                     /locus_tag="PK576_00780"
     CDS             complement(18386..21769)
                     /locus_tag="PK576_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE60156.1"
                     /translation="MASRKHRFFTTPWQLIRFFLPVVTLLTAVVYAVTLFRHLYPGTP
                     AFLTAMAAGLCQQNDLAHPLFSLIIRPIAQLPYATLPVRLNLFCAACGTLAATLFYLL
                     TARLIFIWAAENPGGSMAALPPGTEGIREDEGDLPNRNRMSFPLPASEMPILPASVQA
                     HNWRSARAAALGGMGAALAFAFCGPFWIAATRLYPFAFDLALFFLLLNLLIAYDQKAH
                     PFFLFASSFLLAACCVESPLFLLLLPVGSGFLLRSLLLNEHATFYRTLAVVLTGLIGF
                     LTGGMLLWRASALCAAVPIPAPRPILNVLTATMLDELARWVPSFGWSYIFMQLLFPCA
                     IALFIFAFSFRKRTPLLFLMQLTLAATLIPSLLNLRMSPWAIARMTSRIPVFSSTIVA
                     FLTGLLIAVWYLMREMFDEQLSEDLDYYEYRDHPLVCRIGRWLCWPLLALACLVPFRS
                     FTDIDPREGAFVDVVTDRIYATLDTCDWIVNSSTLTHHLMLRAFQDGRRLRLIETEES
                     PDGANAKHLGTAIREDPSLTRIQARLLNAADLSPVSFLREWLTQETNAYSRIALFRTP
                     VFWRENGFTPLPNSIFFNGLPQDVSVDLAQLRDRHQAFVEFLQPHLFPATPDSIRLFA
                     KIRAALAGQLACAGNELAVLFAGQKRFEEAAELLAQTEALMPDNLSVLLNRYHLALGH
                     GLRADTLPALQTRLREIPRRYARTHLLTPASVQAQTGTLISPDILEQVRKSYWTKSGL
                     LQQLSHTLSPGQPDPFLAVRDKKRELYQTIAKHLETYEFDIADLQLNLLLDIDDRDRF
                     ALVNKARLAIERRDLPEAGLWMDLAKENGVTPAELIWHEAALLILDGKLPEARERLNT
                     ALNAAPADIGLWGLLAEILLQLEEYPELEMRVFPVLRSASVKKEHYLMYKVRGYLLRH
                     NGTEHYRAARANFLRALELNPNLNDVREEILRLDDVLEVPAFSEQDACAILRQNPDHA
                     FANYLWGRVRLERGEVDLAEDLFRRSLETERNAPAFAGLGTVLLEKGEHALAEKFLRR
                     ALDDAPQRLDAWHALARLLLATGRADEASRALDAVLQAKPDDFSARLTLIRIRMAQRN
                     LREAAEQISGLLEQEAHLPRHILRELKPLSQQLLRDLAN"
     gene            complement(21779..23932)
                     /locus_tag="PK576_00785"
     CDS             complement(21779..23932)
                     /locus_tag="PK576_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60157.1"
                     /translation="MKTLLLFCCLLASGLGAADLYVAPAAKTSEPPDGSRRRPFTSLT
                     QARDAIRAARKAGCSEAWTVHVDTGFYPVNEPIVFSPEDSGTADAPVTYRGRGADRSR
                     LCGGRVITGWQVQPDGRWLADIPCDASGTPAYFETLFVNGRRAPRARHPNRGFFNPAA
                     VQQTILTNASPRGVYARATLTGRAGELDPLARSPQPEWRFAQVVVHHKWDTTRRMIAG
                     FDVGKATLITQGAPWKHWNKWQTNSLYYVENVRSALDAPGEWFYDGVNRKLHYLPLPD
                     ERPERAEVVAPLSGVKTLVVFQGDPVAGVFVRHLTFENLGFLYTDSPRRLNQIACAFL
                     PDEICGPLNAPGPTQFEPHQAAAHTEAAIMADGAHGVTFRRCDIAHTGEYGLWFRAGC
                     VSNRVEQCALTDLGAGGIRIGDPGAKGVSASTNAAVQALTSFSTAFNTVDNCIITHGG
                     RFHASGTAVWIGHSPDNSVTHCEISDHFYTGVSVGWVWGYRGSAAQRNTIAFNRIHTI
                     GQRMLGDMGGVYTLGTSFGTCISNNVIFNVDSYTYGGWGLYPDEGSEGIVLENNLVYD
                     TKDGSFHQHYGRDNVLRNNILAFSRECQVAVTRVEPHRSLTVERNIIIWENAPNFSDT
                     RYTRSIQAKVDWQSNLWWRTDGPADFKGMTFAEWQAMGRDTDGLVADPLFENAAERDF
                     RLRRGSPAEQIGFRPFDFSRAGVYGDRAWRRRAHQ"
     gene            complement(23962..24792)
                     /gene="kduI"
                     /locus_tag="PK576_00790"
     CDS             complement(23962..24792)
                     /gene="kduI"
                     /locus_tag="PK576_00790"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOE60158.1"
                     /translation="MRNIETPDRQSYRRMTTDELRNRFVIEDLFQPGQVNLVYTNTDR
                     AVVGGIVPETEHLELTGGREMACSYFCERREVGIINLGEPGTVVVDGTEHALARRECL
                     YVRRGSRGLLFSSANPQKPACFYLVSYPAHTDYPTVKAGLKDANRIELGTLAESNRRT
                     IYQYIRPGFIRSCQLVMGFTMLHEGSVWNSFPPHTHDRRTEVYCYFDLPPNGAVLHLL
                     GAPDETRHVMLREKQVVLSPAWSVHCGAGTGGYAFVWAMGGENQEFDDMDKCDLASFR
                     "
     gene            complement(24914..26119)
                     /gene="hydF"
                     /locus_tag="PK576_00795"
     CDS             complement(24914..26119)
                     /gene="hydF"
                     /locus_tag="PK576_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOE60159.1"
                     /translation="MSLNDIPQGERVHIALFGRRNVGKSSLINALTGQEVSIVSAVKG
                     TTTDPVRKAMELLPLGPILIFDTPGLDDESELGALRIERTRDVLNKTDVAILVLEADS
                     GIGDCEREVLDLVRAKGIPLLTVFNKVDLRPPSPSALRQAAEECKTPLLTVSSVTRAG
                     IEDLKLALAKVAPEDDAERLVGDLLKPNDLVVLVTPIDSAAPKGRLILPQQQTVRDIL
                     EAGALALVTREFELRAALATLARPPRLVITDSQVFESVAAEIPRNVPLTSFSILFSRY
                     KGDLAEQVRGTRAIASLKDGDRVLVAEGCTHHRQCDDIGTVKIPRWLLRHTGRKLVYE
                     HTSGMNYPSDLSPYALIVHCGACMLNRREMRYRIHQAKAAGVPIVNYGVLIAHLHGIL
                     ERVIEPFTQ"
     gene            26336..28585
                     /locus_tag="PK576_00800"
     CDS             26336..28585
                     /locus_tag="PK576_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE60160.1"
                     /translation="MRKIRFLVWVMGASLAFGRGESWMPKPPAPPSVATLTNEARYVE
                     WKFRGDVTDPSTGLAAHALRTNALALASGLEPKEDWRVVKAKVYAMLCDQMAIDVSPL
                     DWYPAISIWNRYDRPMSAVVWRRNGEIARKCYPDITGRINKGNRDGIWRVWKDFDHSV
                     PVWEDMFKLGFAGMRDRVDAHDDGTPFYRSLRITADASIRLIERFAAQARKRLGTADG
                     KSSRLVKQIAALDQLAKGPPQTAYEVLLFIDLYFFMCEHLDAMQCRSLSIIDRNLWPY
                     YQADLAAGRTTEAEFREQFRHFLWQWGSVDNYWGQPVTMGGTKADGTTEYNPLSRIIL
                     DVMDECALPTPKFHVKIGKSTPREILDKLLSMARRNRSLTFIGEESARRILEHYGATK
                     EEARTFYTTGCYEIVLANGANKTGVGYINLLKQVEFVLRDAKGGKFSAADYAAFQAEC
                     IRRMVNVCTNCLAITSAFEQHLDDVNPANITTLGVAYAVKTGRDAFANGSERGNNTAI
                     LIAAMGTTVDALVAVKDLVYEKKEMSLADLGKLMAANWEGRDDLRLRMKRSKRKWGNN
                     DPDANATAAAVSKALAAAVNGKPNARGGIYVLSGHSARAFIYEGAATGATPDGRKAGE
                     EFSKNMSATMGADTEGVTALIQTLGALDSRDWPGDFPLDVMLHPATVEGDRGLALMRT
                     LLDVYYANGGLQIQFVVVSADELRDAQKHPEKYENLQIRVCGWNVRWNDIPKAEQDAY
                     ILRAESIMQ"
     gene            28582..29409
                     /locus_tag="PK576_00805"
     CDS             28582..29409
                     /locus_tag="PK576_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE60161.1"
                     /translation="MNRREFIGSSAAFAVAPLFAAGGGKVRTPLMLDTKRMAIWDGPG
                     VRTTFFVKGCPLKCIWCHNPESISAQRQTARFQHLCRRCEKCTMDEATCPTRALKFYG
                     KPMKIDEIVAKALEDKPFYDSSGGGVTLSGGEPLLFWEWLAELFTAFKKAGLHTCLDT
                     SLYAPPVAIEAMLPLVDMWLPDYKADDDARHRKYTGVPNKIIRKNLERLVAAKAKLEV
                     RLLVVPRCTDDDDLAARHAYLDSLDIPRAAQVELEYRDLARSKYLALGMPDTMPPST"
     gene            29623..31752
                     /locus_tag="PK576_00810"
     CDS             29623..31752
                     /locus_tag="PK576_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE60162.1"
                     /translation="MRLHENCRMNNFKLGVVALGGVMAGTILARPVLQPEVKEYVSSK
                     GVADYAAAPVVYQDQRQCRIGADEIPSTGEKTAYAGHALPEAGVFIAIADSALGKKLV
                     ADFRLDVPAKKQGYAIRVDGKCAVIVGHDAIGALYGAVTFRQMAAKGGRVECAMVRDW
                     PDVLYRGGASLGRGLWPYGKSDGVDGIKAALDVMMRHKLNIISDFFGLDELAPREKLA
                     TWRELVRYARDRGIYSYYFISTALFRRTNKPEGMTLEKWPCVKDLRTWNEWYYCWSDD
                     EGTEKAVNHCADILQNIGAEDGIVQIHPVDGGGWKDPEFWSKRCAKCRSLWNDHERWK
                     ATVHQLEIWNRVLRRRCPNVIVGSCIYPYTYSALLTPEKDRTDLWRESHTEYWSKVSE
                     GIGNKEFFFSSWMSPPWLAEQIRRYVPSRPIHFAQYPQFSGIFQTTSRKACSVYEENA
                     DNTYLPQGTDFDAVQWESLLLGVESTWNRNMPGAEAFDGGTYYDAAVDHTGPRIVMEQ
                     VLPRLCAAFWGTELAPYMTRILSSGVLPSYIRDPGKALAYWNSVRKDPLFDPTVEQVS
                     TLERKRIEKIVDTSERMREQVKAAETVIRDLTEARGHLDGLDSVRRRYFMKLAKYAPF
                     WLATARARYALRAANDLIAAGRHEEAKRALPEGLRQLREEWRQAEENLKALSSEVDCS
                     PRRYVWPVKLETLVAEFERAETSAGRP"
     gene            32017..34218
                     /locus_tag="PK576_00815"
     CDS             32017..34218
                     /locus_tag="PK576_00815"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOE60163.1"
                     /translation="MASRRMFFALIILLSAIPDRTARAVSAEWTLETVDRNARGVADW
                     ARPNPKRSLVWENGTLYAGITALGLADPSLPYLDQIRQLGAHHAWGHRERTYHADDHC
                     IGHAWLELAAFDNNPVYAARLRRNFEYILANRHRGSLNWSEKDCAKRWCWSDALFMAP
                     PVWVKMYGLTGDRRYLDFLDQEYKLTTDLLYDKEASLFYRDSRYLTERTKNGKKVFWG
                     RGNAWVYGGLALMLRDLPADWPARADYTKLFREMSAALKKTQGTDGAWRPSLLDPDDP
                     DLPEMSCTTFFVYGMLWGVNNGVLDKETYLPGIKKAWAVISASINGEGRLGWTQPPGW
                     APEKAFNADTHAVYAIGSYLLAAMELRKQIVADAHPERKTVTATNPSERFRSAETLSL
                     NWGELKLDAAKTRVFDVRNAAFLPHQLIDSDLDGVPDELIFRSYFAARQTREFWVFED
                     SGIPPPVSPEICFSRHVPERMDDFAWENDRMAYRIYGPAVSLPPPQGEGLHSSGIDVW
                     CKKVRHPVINTWYKHRRYHDDHGEGMDFYKVGPGRGCGGLGVFHAGAFHVSGNWATWK
                     HIANGPVRTVFEVTYAPWACGNGAQVAETRRVSLDAGSHFTRFESRLTLTGATGLAAG
                     PGMDISVKNGHNGLLTCRPDQGWLALYGPEQKGAGAIATAILLPAAGNLMGDGGKNVY
                     LVDAVSPDKPLVWYAGAAWSGAGDFVRPEDWTEQVSAFAAALAEPLVVTVR"
     gene            34320..35972
                     /locus_tag="PK576_00820"
     CDS             34320..35972
                     /locus_tag="PK576_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE60164.1"
                     /translation="MRTRRWVAVGLMLACACRAESLLRPNDRIIFVGDSITAQAVGEM
                     QGYYHQFTNALKTAYPASRNEVIGLGFSGHTVFSWADSLEVKSRTESVPANTGGFDVH
                     TAFARPADLVLVLLGMNDILRPTVTDEEASLRMWQDKYRELIRAIRKRVSPREFVLCE
                     ITPLTEDPLAPKNRVRDRMTQLVRELAREENCRVAETGGALFDVIARARKARQDYLVI
                     PDTVHPRRPLGHTAIARGMAAALRDAKLTALFDARLDAAITGLRPAQATVTWRFKPQP
                     GIEAKGGEQVYRIECYWDDGTGASVARVPTFSLEVPEGWLVAEAPVTRTEAVFTVRGT
                     PERLQTPLTILAKAGEEVIRRTVQIPVPWRVVCGPENGGAWMSGRRYQPDNRVPFMET
                     DLVAGKHFLEPFTDGGKSYGWQINTPCMDYTAGDDPNAVVPYSLTFGRENDVLYAVRW
                     VYSAKARPVQIRLSHRTFSATLGFVVWINGERVMTADLNRSGKNQAVGQATLKAGWNR
                     LLVRNDHLQWQRQFACALLPVEGDALDDLRYAITPQSVSPSP"
     gene            36280..37242
                     /gene="trpS"
                     /locus_tag="PK576_00825"
     CDS             36280..37242
                     /gene="trpS"
                     /locus_tag="PK576_00825"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009511994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOE60165.1"
                     /translation="MRILSGIQPSGKLHLGNYFGMMRPALRLQEQGEAYLFIANYHAL
                     TSVHDPERLRQDTLDVALDFLACGLDPERTVFFRQSDVPEVHDLAWLLSIVTPMGLLE
                     RCHSYKDKLTKGIAPTHGLFAYPVLMAADILLYQSDVVPVGRDQKQHVEVTRDIAIKF
                     NNEFGEVFRIPEPAISEEVAVVPGLDGQKMSKSYGNTIELFGDPKEARARIMRIVTDS
                     KGLEEPKDPAACNIFALYRLFADPARRAEMEANYRGGNYGYGHAKKALAAIFEETFAP
                     FRARREELQRNLDYVEEVLRKGAARARAEAAKTMDRARRAVGIA"
     gene            37281..38099
                     /locus_tag="PK576_00830"
     CDS             37281..38099
                     /locus_tag="PK576_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOE60166.1"
                     /translation="MSIEALLPDEYKVDLEVFEGPLDLLLYLIRREEIDIYDIPIERI
                     TAQYLTYLDVMRMLDLNIAGEFLVMAATLMMIKSRMLLPVETRAVEEEESDETWVDPR
                     LDLVRQLIEYKKFKDAANRLLEWETLQGEVFAFGGAQPILERDPPDAGQALGDIGLFD
                     LLTAFQEVLARAPLEPLGHIEPIRWSVPDKMETILTLTRGGNRVAFSALFNPESHRGE
                     VIVTFLALLELLRLRQIRLWQNAAFHEILILPAEEMDGGPLPVPVIEGDEHVQA"
     gene            38086..38880
                     /gene="scpB"
                     /locus_tag="PK576_00835"
     CDS             38086..38880
                     /gene="scpB"
                     /locus_tag="PK576_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOE60167.1"
                     /translation="MSKPDEVKVPLPASLQQVVGALLFGADHPLTVEEIRTCLKNVAE
                     DASGEEKAALSIFAEASPREIEDAVGGITKELSRLGLGIEVTAVGNAFRFQTQACCGR
                     WVRSLLKAERPSRLSRPALETLAIIAYRQPIAKSEIESVRGVTVDHIVKALMELHLIR
                     IVGRSELPGRPFLYGTTASFLSHFGLGSLKELNDLDPTLQRSKPSERKAIHQKKKKKD
                     ESKDASQDAALPSDPPASEKGATEAVNEILGDGSPPGENAEPLYEA"
     gene            complement(39555..39845)
                     /locus_tag="PK576_00840"
     CDS             complement(39555..39845)
                     /locus_tag="PK576_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60168.1"
                     /translation="MNRILWDAEARRRLSLRHLRALQISVIALVTIAIISNTVVVAKS
                     KTGNVEVSQFLCVTMCLWVLPAALYRIAQLRRIACIRTDDHNVLTRVDTRRC"
     gene            complement(39987..41039)
                     /gene="purM"
                     /locus_tag="PK576_00845"
     CDS             complement(39987..41039)
                     /gene="purM"
                     /locus_tag="PK576_00845"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOE60169.1"
                     /translation="MTKRTTPNRAVSAYAQAGVDIDSKMSGIEIIKKMVGATTTKGVV
                     GGIGNFGGLFHSPGKDHLLVASTDGVGTKLKMAVLAKKHDTVGQDLVNHCVNDILVQG
                     AKPLFFLDYIGMARFDVDIFRDVVAGLCKACRENGCALLGGETAELPGLYPQGEYDLV
                     GTIVGTVERKKLITGTSIRVGDVIIGLPSGGLQTNGFSLARKVIIDQCGLTPQDLIPG
                     TRMTVADTLLALHRSFLKPVTALQAKVPIRGMAHITGGGFVDNIPRVLPPTCNAVIDR
                     NSWQPPTVFTFLERQGKVERDEMYRVFNMGIGFVIIVRRKDLTAALKMLKAQRQAPVV
                     IGMIEKGSKKVVYRDR"
     gene            complement(41313..41993)
                     /locus_tag="PK576_00850"
     CDS             complement(41313..41993)
                     /locus_tag="PK576_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60170.1"
                     /translation="MGKTGKSKRRANVTGTGQAAGVPVAERACGDAAEAVSFSDADSR
                     TTAPEPLSDRSDGIPEPAPVVKPLFPIGSRWCRIAPDLTKTAIALAVIVLAAAIWYKP
                     PLIRANFPYLSYRFDNRVLTDAVLYRPLAMPTRYYIMLPAQRTGRYAWFAVDRRREVV
                     ALAEAPQHRFLGQPAIRRSDPLGLDLEFRKLDGSEWRIFFFDDAIVFSNNVLAVRLDI
                     STDARADP"
     gene            complement(42113..43078)
                     /locus_tag="PK576_00855"
     CDS             complement(42113..43078)
                     /locus_tag="PK576_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE60171.1"
                     /translation="MNRPMQRRDFLRTALSAGALVATSRLPSLADDPALFPKRGRFER
                     LSLAYVHIDAGATRPFSILHLSDTHFTAAYPHEDERKQKLKAARTQTFGGRQEEALRD
                     SLAWAKDNVDFVLHTGDLIDWLSEANLDLVKQSFGAGMLGALGNHEFSRYMWLEPSEN
                     TEAYKARSAELLSKAFPFDLRLHAQVVNGVNFVTLDSVYGYVCPDQVERFRAEVKKGL
                     PIILCMHVPFYTDGLWRASRKFWARSGKKFESAVIPDASGDFKTQQTDPTTRDFIAYL
                     KTEPLLKAILAGHLHITVQDRFSPTAMQYVVGGNFLFHGQEVTFA"
     gene            complement(43119..44468)
                     /locus_tag="PK576_00860"
     CDS             complement(43119..44468)
                     /locus_tag="PK576_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60172.1"
                     /translation="MMKTSRLFVSAAAFILSASTVVHAATLHVSAATGGSVRKADGSK
                     EKPWRDLQAALDKAQAGDTILVAAGNYLGTSDQGFLKMTIPVNIMGGYTDDFASRDVL
                     KNLTMVQPTTAQNGTSRSFALLSIGDPNKPRSFNAGDGGVTIDGIVFDRGFSNGYHPT
                     KGKPKDVETGMLVNPPGQGINGDAKNVATIVQPLIYFGNGFGNVTIRNCTFANSGNYA
                     IRGSWSQGKVVIENNVFINNTYAAIELSGGGKSGEFSLTIDCAYNTMLFNWARTNDLG
                     DMGYGFRYMNNVHSDVHHCIIGTSTLGGLDRARIENSKDHEKMKKTGAENNAFFLNTQ
                     GDLVLPGGAKWLLIRAKDFEDREELYKYEGNAELPAEALKGKINEAYLNGFVSANNSE
                     SVLLDRNSPVNQVRSAFGMNLQATGTTRVDMFANRYPLTDALKLFGAVEGKGAQMPK"
     gene            complement(44542..45747)
                     /locus_tag="PK576_00865"
     CDS             complement(44542..45747)
                     /locus_tag="PK576_00865"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HOE60173.1"
                     /translation="MAYQEVTRTGYGDRLKRALGGVVGGAVMFIGGTALLFWNEGNFV
                     KTKKALNEAQDAVVQVEQVTSLDPALEGKLIHASARAETQDVLEDAEFGVKQTAIQLR
                     RSVEYYQWVEESRTETRDRMGGGEERVTTYTYKKEWRSKPVDSGEFRDPDYQNRNTVW
                     MTFDAQTSYAKNVSFGAYTLPQFFVEAIGQAEHANVNPAPGQIQAWAKSMSAHPRFPR
                     ALSSTNTPPIHVSGNTVYFGRSPGAPVIGDVRVSFTWVAPHEVSIIGKVSGKTFVKFV
                     AKNGKTVSRLDDGVRSAEEMFASAAKENAILTWILRLVGVLCVCIGLKSIFGILEAIA
                     KVIPILGDIVGAGVGLVCTLVGLAWSFLWISIAWLAYRPLIGIPLLVASVGCVVWLKT
                     KAKAKEVRI"
BASE COUNT         8948 a        13394 c        14853 g         8849 t
ORIGIN      
        1 ccacgagaca ctttcttggg tttcaagggt tcaggtttca tggtttcatg gtttcatggt
       61 ttctggtttc atggtttcat ggtttcaggg tttcatccaa atcggggtcg gggtcgctat
      121 cgctatcggg atcgactgtt taaccacgag acactttctt gggtttcatg gtttcagggt
      181 ttcagggttt caggggttct ggggttcatc caaatcgggg tcggggtcgc tatcgctatc
      241 gggatcgatc atttaacctc ccgtcagcca gtagcaagag cgttgctctt gctactggct
      301 ggcgggtcgc gaatgtttcg cgtgagtgtg gaaaggggtc agaatgaaac acatgaaagg
      361 aaactggttg atgaatcgct gtgctgttgc gtgcggctgt cttgctctgg tgcaggccac
      421 ctttgcgggt agcgcgacgt ggaactatcg ggagggggcc ggcggctcgc cggcgaaccc
      481 gacactgtgg accgataccg cgaactggac aggcggtgcg gtgcgcctga cgggtgacac
      541 gtccaagttt acgacggggt cgtacaagtt gattgcgtcg tcggggttga tcccgggcag
      601 tgaatggacc gtcgatggct atacgggccg caaggtcctc aagctgaggg ttaaggcaga
      661 cggtctttat ctggaggtga tcgagcaggg tctgagtgtc gtgatccgct aaggtcggtc
      721 aaaggagttc aaacaaaaaa aggaacctgt tatgaaaaaa gagtgttttt ctctctgcct
      781 cacggttgtc gcggcagtgg tggtgggatt ggacgcgcgc gcggccaaca tcgagtttta
      841 ccggcgttct aaaaccggtg gcagttggaa tgacgtcaac cactgggtga cgaatgctgg
      901 cggcagtgtg ctcatgggaa cgtatccgaa ggatcccgcc accgaccggg tctggttcaa
      961 agacgggttc accaacgaca cccaagagct ttatctggag tcggatatca atgtgttgaa
     1021 catgatgcag ctcaacgcca gcgccgggaa tcaaaccaac tacctcaacc tcggcgggca
     1081 cacgctgtat gtggtcaact tcctcaatca ggactacgga tgcgtcggca cgttcgccat
     1141 taccaacggg aacatctcct ctatgatgat ccgcaccatc ggcagcggat attgcctttg
     1201 gttcaaaggg ccgaataccc ggttcgatct ccatggcgac tggtaccgga tctgcatcgg
     1261 atataacagt gctagaagca ccctgcgggt ttccgacggg gcggtctgca actggccgtg
     1321 caccatgttt tatctgggag gagagcagct cagcggcaag cacgacggta accggctggt
     1381 gattgacagc ggtggtttgg tgacgaattg caatacagat ctggtaatca atcgaggcgg
     1441 cggcaccaac gggctttatg tgtcagacgg ggggaaattc cactccaacg cgggtattca
     1501 gatgaactcc agcgatggcg gctggcatac gaatccggtg atgaaaagcg ttaccgcaat
     1561 cctcgatgtg cgtgacggca ccgtcgacat cagaagactg acaggtagcg tcggctcgaa
     1621 cagcgtgttg cggttggcag ggccgcagtc cgatctccgc atccgaaacg aaatggtcat
     1681 caccaataca ttcgacatga cgttcgacat gacggacggt gtgttcgccg ccgaaacagc
     1741 gccgatccgc ctcaccagta ccagccatac gcacattctg ggtgacggca cgatccgggt
     1801 gcgttacccg cagcgtttcg cgaaagagca caagggcctc attccgctca ttcacgcggc
     1861 ctccggcaac ctcacgtaca cgggggatat ccggttcgac ttcaccgaag cccgctgggc
     1921 ccaggcagtc acgaactcga ctatggtggc catcaagatc gtgccgcccg ggttggtgtt
     1981 cagcatccag tgagcggcgg ccatgaggta agggattgct ttgtccgggt aacgggggcg
     2041 aatgttgaat gaaaacagta tggaagacca gccgaggatg gggggccatc gcgttgagcg
     2101 catgggtgtt atgtgttcgc ggtgcgattg tggatccggc ggactacggg ttctcgccgg
     2161 atgcgcctgc ggcgacgaac gccgtggcgc tgcaacgggc gctggatggc ggcaagcgga
     2221 ccgtgcaggt gacgcggccc ggtgtctacg gcttggatcg gacggtatgg ctggacagcg
     2281 atacgcggct gattttcacg cccggcgcgg tgctgcagaa gcaggcggca tatgtccatg
     2341 tgctggccaa ccgtgggctc gagtcgtggg cctgggacac gaacatcgtc gtggagggcg
     2401 cgacgatcga cgtcaatgac ttcagcgccg gtcatggcga ggacagtccg caatacggca
     2461 accgcggaca gatctccttc tgccgcatcc gcgacctcac ggtgcgcaag ttcacttgcc
     2521 tcaacctgca tggcggccag tacgggtttc agatcgccac cttcgagaat gtgatcttgg
     2581 atgaattcga gatccgcggc gcgaaagacg gcgtccatgt caatgcgggg cgaaaattcg
     2641 tgatccgcaa tgggattctg gagaccggcg atgacgggat cgcgctcacc gccatggact
     2701 gggtttcctc cgcgccggtg gtgggggaca tcgaggacgg cttgatcgag aacatcacgg
     2761 atgacggccg cggccgttgc aactttagcc gcattctgac cggcgcgtgg acggactggc
     2821 acaaggggat ccggctacag cgcggcgaca ccgtgcggtg cggaaagaag ctctaccgcg
     2881 tggcgatgcc gcggggcacg aacgagtacg tctcgctgga ggcgccgacg cacacgcgcg
     2941 gcatctggac ggacgcgacc gggctgaaat tctggttcat gcaggatagc gacgcgctcg
     3001 ccgcgaacat ccgcaatgtc acgttccgca acctttacct gcgctcgccg aggaacggtt
     3061 tcctcgcgga ctgggaaacc cgctcgtcat ggaaccgcgg gattcatccg gaggtgaagc
     3121 cggagaacta tccggtctgt gaagtccggc tggaggatgt ttactccgaa tcgccgggtg
     3181 cgctggtcag cggttacgag tccgtgcgct tgtggctacg gaacatcacc aaaacacggg
     3241 gaccgctcgt gaaccttgcg cgtccgaacg acaaccggga ctgcgcgatc gacgtctggc
     3301 tgcatctctc cggcgtggtg ttgaaagccg atgacgggaa aggggcggac atctctgtgg
     3361 gaggggcggg ctcttcacta aacctgacgg tcgagggcct gatgcaggag cgtgatctgc
     3421 gggtgtcggc gggtgcgggc gtccgtgcgc gcgtcaacgg aatcgggtcg atccactcgc
     3481 tggagggcct gacgccggag gcaggcgact ccatcaaagt gggcggcgta ttgaaaacgt
     3541 tcgacggcac acagtggcgt tgacgcgata acgtttcggc gttttctttc gtcagcctga
     3601 cgtacagagt caggtcggcc tcagcggatt ctgcgggcgt ggcggcggca gcatcgggac
     3661 atcggtgtcg aagggcggct tttcgaacgc ggcgtcgagg atctgctgca ggagctgcgg
     3721 gtaggcgatc cccgcagctt tggccgaacg ggccagcgcg acgtcctcct ccagacaggg
     3781 gttgcagttg acctccagca catgcggctc gcccgcctcg tcgaggcgga tgtccacgcg
     3841 gccatagccc cagccgccca aggcgcggaa cgctttgacc gtcaggttgg tgagtttgcg
     3901 tgccagcggc ggcgcgacgt ccgccgggca gatcaccgac gtgcacttgt attcgatact
     3961 gctctccgtc cacttggcgt cataggacat gatcggggga atgccggggg gcaacccggc
     4021 gtagttgact tcggcaaagg gcagcacacg ctgtttggct ccgccgatga tgccgatgtt
     4081 gaactcgcgc cccggcagga agcgttgcac ggtcgctggt tgatggtagt cgcgcagaat
     4141 gatgccgacc tggcggcgca gggcggtgac cgagtgcacc acggagtggc gcgtcagtcc
     4201 gacgcccgca tgctctttgg cggcctggac gatcaggggg aaacgccact tcacaccgga
     4261 cacgtctttc acgcgctcca gcagccgcgc gtcaggcggc accggcagac cggcgccttg
     4321 cagcagactg gccgccatgt gcttgtagcg gcagaggccc agcgccaagg cgtgcgagcc
     4381 ggtgatcggg aaacccatca tacgcaccag cgcggccacg cgcatctcat aaagcgcgct
     4441 gtgcacaaca tcgtcgtatt ggttgaagac cacgtccggt tgcaggcgcg ccagcttacg
     4501 ctggaacccc agcatgtcgt tggccagcgg caacacggtc acgcggtgcc cgaggctacg
     4561 cagcgcgcgc gccagactgc ggatgaaacg cctcaaatca ttcgtgctgc cgcgatcatc
     4621 cggcgcctcg ggaacggcgt cccggtaggt gttgtagatc agggcgatgt gcagcacgcg
     4681 tttccgtttc atgggcgcat tttaacaatg aaaacaaaaa gtgcaatagg acgacgtcca
     4741 acgttcccgt tatccccccg gtttcgcccg gcaccccgga cagtcggctt atctcgcgct
     4801 ttactgctcg ggcttcacct cgcgggtcat gagcgcctcg acggcatccc gcgccgaaag
     4861 cccctcataa cagagtgcgt gcacaacctc ggtgatcggc atggaaacgc cgtgggtacg
     4921 gctgagcgcg tggatgattt tggcgttcca gacgccctcg gccaccatct tcatgctgtc
     4981 cagaatctct tggatccgct cgccgcggcc gagtcgttcg cccaccatgt gattgcggct
     5041 gtggcggctg gtgcaggtga cgatcaggtc gcccgcgccg ctcagtcccg agaaggtggc
     5101 gggacacgcg ccgagcgcgg tgccgaaacg ggtgatctcc gccaggcccc gtgtgatcaa
     5161 cgcggcgcgg gagttgtcgc cgaatccgag tccgtcggac acgccgaccg cgatggcgat
     5221 gacgttcttc accgcgccgc cgatttccac gcccaagggg tccggggacg tgtagacgcg
     5281 gaaagccggg ccgctgaaga gctgctgcac gcggcgggtg agttcctctt cctcagaggc
     5341 cgcgaccacc gcagtgggga gccggcgcgc cacttcctcg gcgtgactcg gtcccgagag
     5401 caccacgatc gagcggagcc cgagaatcgt gcgggcgagc tggctcatcc ggcagtgggt
     5461 ggtctcgcac aagcctttgg tcagactgac ggtcagacag tcggcgggga tcaggccttt
     5521 gaaccgttca caaacctggg cgaggaattt ggagggcgag gccaggatca ccacgtccgc
     5581 acctgccacg gcttcggccg cgtcggcggt ccagcgcagg gtttcgggga gcggaatccc
     5641 gggcagaaag cgcgtgttct cccggcgttc acggatttca tccacaactt ccggaaaggg
     5701 gccccagagg gtgacggcgt ggccatagcc gttgagtaga aggccgacgg ccgtacccca
     5761 gccgccgtcg ccgatgatgg tgatgtgcat atccgtatct ctcccagagt ctgcgctcaa
     5821 acctgccgcc gtgtacgaaa ggtgaaacgc gcttctgttc cgttgcgtat ccgtgcgagg
     5881 ttggaccggt gtttccagac cgccaccgcc gcaagggcgg tgaacacggc cgggaaccag
     5941 aacccgtacg ccgcgtggag gcgcagtggc caccacacgg ccgcggccag cgtcacggcc
     6001 gctgccacag aggctgccga gaccatccgc gtcatgagga agacaaccag ccagacaacg
     6061 aaagcgatgc ccaccgcctg gggcgcgagg ccgagcacca gacccgtgct cgtggccacg
     6121 cccttgcccc ccttaaagcg gaggaaacag gtccaattgt ggccgacgac cgtcagcgcg
     6181 ccgcagaaca tcgccagccc cttgccccct gccgcgcccg tttccggcgg acagattgtc
     6241 aaggccaggg ccggaatcag cgtgacgccg ccgaggcctt tcaaaaaatc gagcgcgaag
     6301 gtcagaacgc ccagcggctt gctgactgaa cggaacacat tggtggcccc gatgttgccg
     6361 ctgcccagcg tgcggatatc ctttccgcgg ctccgggcca gcaggaatcc gaaggggatc
     6421 gatcccaaca ggtaagcggc cacgccgccg acgatccata cggttacggt tgtcatggcg
     6481 gcagtgtagc aaaatctggg cgaagcgggc agacacggtt aaaaaaaaca gacctctgaa
     6541 taaaacgggc aaatcccgct ggacacggat acgcggtttc cggtataaag cgttactact
     6601 tgaagtaacg cccggaaggg cggttcaaaa ccacaacagc cccaaacaag ggatccatat
     6661 gaaaagcatg ttgcgatggt tcatcggtat cggattggcg ggtgtcctgt ttgcggagga
     6721 gaagagtcct gcgggacagg gtgcgggcgc ggcggcgcct gcggacgtgg cacagatgaa
     6781 ggcgcgcgag accggcggac tcgattggga cgcgggtgcg gatctgcgtg tgcggcagga
     6841 ggccagtgac aacctgccga agcggatcaa tgcgcagggc gagggcgtga cggccaaaaa
     6901 cgacaactac ctccggatcc ggccgcgcgt gtggggtcag gtgaagaacg aggatttcaa
     6961 actctatctg cgcgtcgccg atgagttccg cgagtatttc cagccgagag agagccgcaa
     7021 tgcccgcccg ccggacgagg tgctggtgga cagcctctat ctggatctct acaatctggc
     7081 tgacgggtgg ctcgatctgc gcatcgggcg acaggatttc ttcggtccgg acggtccggt
     7141 ttacggcgcg ggccgtgtga tctgcgacgg cacacccttt gacgggtcgc gcacgatctt
     7201 catggatgcg atcaaggcca cggtgaagtt cgacgagaag aacagtctgg atctgctggc
     7261 catctacaac agcgcggaaa ccgagatgag ctggggccat ccgtatcgca cggatggcag
     7321 tgaatacaag gagcgtccgt tgacctcgat ccatcccgcc agcaaggata tggacgaata
     7381 cggcggcggg ctttatttcc gctctcggtc ggtgccggaa gtgccgttcg agttgtacgc
     7441 tttctacaag cgcgagacca aggcccgcct ggcgggcggg gcgcgtttgc cgggccgcaa
     7501 cacgtacacc tggggtctgc gcgtgatgcc ccaactgacc gaaacgcttt cggccgaggt
     7561 cgagggcgcc gttcaggcgg gcgagaaaga cggcggggcg tcgacttcgg gttacatggg
     7621 gtatgcgggg ttgacctatc ggccattgat cgatggttcg tccaaaccgt tcttcaccgg
     7681 ctcgctctac tatctttccg gtgacaagaa ccgcgatgcg gagggggata acgacaccgc
     7741 ctggaatccg ctttgggcgc ggtggccgca attcagcgag atgtacgtct acagtttcat
     7801 gtacggtgtc gggtactggt ccaacctgct ctatcccagc ctggaggcag gggtcggctt
     7861 cgcgccgggt cacaaggtgc gcgcgagcgt cggcccgatg tatgtggcgg tggaggacga
     7921 tctgggcggc ggcaacggcg atctgctggg atggctgggg gtggtccgtt acgacttccc
     7981 gctgttcaag aatatcttcg gcaaacgcgg cgatctgacc ggtcacgtca cggcggaagt
     8041 gctcgacccg ggcgactact atgtatccga cacggtcgcc tatttcctgc gttgggaagt
     8101 gatcgcccgt ttctgaaaaa accgcgacct tgcagatgcg tttttcccgc agggatgcta
     8161 gaatctccgt tatgcaaacc aagatacctt gtttgatgtg ggtggttctt gcgcccctgt
     8221 tgcttcaggc gcgggtgggc gagaatctga aacagagcga gacgcgctac ggcaccgggg
     8281 aggaacttca agacgcggaa acgttcatcc ttaaaaatgc gcgcaatgtc gtctacttcc
     8341 acggcgactg gatcatcacc gcagggtatc tcaacaacgt gaccgcacgg atccaatacg
     8401 agaaagcggg cttgcggcag ccacgggcgc agctcacccg cgaggatgtc gccgagatcc
     8461 tcgaggccga ggatgcggcg gggtggtccg cctacaacga ggagacccgt caggccggca
     8521 aagcctacac gggcaagacc catccgcctc cggtgttgcg atccgtctcg gggttgaccg
     8581 cacgctacaa ggtgttcagc gtgacggtgg agcgcgaaga ggcgtcgcac cacgagaagg
     8641 ggacgacgct gcggcagccg ctccggccca cgctgacccc gcgcggcaaa cgcgtggccc
     8701 tgtgactgtg ccgcggcggt cgccgtgccg cgtcagtcca gcgtgtgtac cagcaggccc
     8761 gagcgcagct tgggctcgaa ccaggtgctt ttgggcggca tgatctggcc ggcgtcggcg
     8821 atgtccatca tctgctgcac cgtcacgggg aacatcgaga acgccacggc gtcctgtccg
     8881 ctggtcacgc gttggaccag ttcgccggtg ccgcggatgc cgccgacaaa ggcgatgcgc
     8941 gtgttggtgc gcgggtcatc gatcccgagc acagggccga gcaggcggtt ctgcagtacg
     9001 ctcacatcga gtcgccccac cggatcggcg tccacggcct cccaggtgag ccggtgccac
     9061 gcgccgttca ggaacatccg tatttcacgc ggtccggcgg gctccgcacc gtcggtcggt
     9121 tcggtacgga agagcgtccg tacccgctcc agaaacgcgt ccggggtcag accgttcaag
     9181 tccgccacgc aacggttata ggggaggatg cgcaactgat cggccgggaa gagcacggcg
     9241 agaaaccagt tgtactcctc gtgcccggta tggccgggat gggcctcgcg gcgctgtttc
     9301 ccggcgcgga aggccgctgc ggcgcgatgg tggccgtccg ccacataggc cagcggcacg
     9361 tggtggaagg cttcgaccca cgcggcggga tcgtcctcgg cgcgccagac ggtatggcgt
     9421 accccgtcgg ctgcggtgaa gtcatagagt ggtgtggcct gctgtgcctg cgccacgcgg
     9481 gcgtcgagat cctcccgcgt ccggtaggtc aggaaaaccg gtcccgagtt ggcgttcagt
     9541 tcaaggcagt ggcgtgtgcg gtcatcctct ttgtcctgac gggttttctc atgcttgcgg
     9601 atcacgtcgc gcgcgtaatc gtcgatgtgg cagcaggcca ccacgccggt ctggctgtgg
     9661 gtgcccatga tctggcggta cacatagatc cgctcttcgg cctcgcgcaa cagcaccccg
     9721 cgtgcctgca actccgcaaa ggcccgcgcg ccctgcgcat agaccgcgtc atcatagatg
     9781 tccaccgtgt cgggcaggtc gatctcgggc cgcgacacat gcagaaaact gtccggattg
     9841 ccggcggcca gcgcgcgcgc ttcggcggtg tcgaccacat cgtacggaac ggaggcgagc
     9901 gacgccgccc ggtccggggc gggccgcaac gcggcaaagg gtttaatcag catggaagac
     9961 tctccggata cgcgaaacgc aaaggaccgt ggctccgggc cgtcctttgc gcttcacagg
    10021 atgggcagcg ggcccgttca gaaaccgatg ttgatcagcg gacagaacgg aacgtcgccg
    10081 ccgcggatga tgttgatcag gccgatctgg tacccgtgca tgtcctccgc ccggttgacc
    10141 aatccgacct ggaaaccctc ggcataatcc gcgaggttga tcagtccgac ttgcagtccg
    10201 tacagggtgc gggattcgtt ccagaggccg gtctgcattc cgagcatgtc cccgagcccc
    10261 gtggagttgt aacacccgat ctgccagccg gccagcgcgt ccagcacgct gttgcgcagc
    10321 aggttgatct ggacaccgtt gaaatactgc gaccgtccga tcagaccgat atccaaaccg
    10381 gtgacctgct cgcactgccc ggaaggcaga ttgaggcgca atcccacaac gtcaatgtcc
    10441 tccgtgtcgt taaacgcaca ccaaaccggc caacgcactt tttccgcagc ctgcgcgccc
    10501 acggcaatca gacaagccag aaccaaacca gaccacattc ttttcatgaa ttacctcgcc
    10561 tcgctaatga acccgacatc gtcttgtccg ctggatcaac ggacacccga acgcgtatgg
    10621 ttattaacta tacagatttt agggtgcggc gcaaaggcca gaatgcgatg ggcgcgattt
    10681 ttttatgcgg tttgcggctt gccggaatgc ctcatactcg ttaagatgac agataatgac
    10741 aattcaaaat gcgggagagg cgacatgaat gtgtggatac ggacagcagg cgtaggcgtg
    10801 ttctgggcag ggttgctatg ggcgggagac ccggtcccgt atggcgcgat tccttcggaa
    10861 cgacaggtac ggtggcacga gatcgaagtt ttttctctga ttcattacgg gctcaacacc
    10921 tacacggacc gcgagtgggg atacggggag gaggaccccg ctctgttcaa tcccgcgaat
    10981 ctcgatgcga accagatcgt gtccgcgctc cgcagcggtg ggatgcgggg cgtgattctg
    11041 gtggcgaaac accatgacgg tttctgtctc tggcctaccc aaaccacagc gcacaacatc
    11101 tccaaatccc cttggcgcgg cggcaagggc gatctggtgc gggaggttgc cgacgccgcc
    11161 aaaaaacacg ggttgcattt cggagtctac tgttcgcctt gggatcgcaa tcacgcggcc
    11221 tacggcacgc ccgcctatct ggcggtctat caggctcagc tccgcgaact gctttcccag
    11281 tacggcacgc tcttcgaggt ctggttcgac ggtgccaacg gcggcgacgg gtactatggc
    11341 ggagcgcgtg aaaaacgaag catcgaccgt ttcacctact atgattggcc caacacctgg
    11401 aagatcgttc gcgaactgca accctcggcc tgcatcttcg gcgatgtcgg tccggattgc
    11461 cgctgggtcg gtaacgagaa gggatacgcc gccgaagagt cgtgggcgac ctttacccct
    11521 aaaggcaaga cggatccggc ccgccccgcc aacggcgact ccatgaacca tatcgagggt
    11581 ccggccggcc accgcaacgg gacggcttgg atgccggccg agtgtgatgt gcccctgcgc
    11641 aagggatggt tctggcatcc ggatcaggac gccacagtgc gttcgcccgc cacgttactg
    11701 gacatctact ttcgcagtgt cggcaaaggc ggttgcatga acctcggtgt ggcgcctgac
    11761 cgcaacggac gcgtctgcga agcggatacc cgttcgctcg aagccttcgg gaacctgctg
    11821 agcgtacttt ttgccgtgaa tttcgccgaa caggccacgg tcagcgccag ccatgtgcgc
    11881 gcctgggaac ctgcgccggg caagacgcat ccctatgagc cggcccgtgt cgtggatcgt
    11941 gatcgttatt cctattgggc cacggatgac ggcgtcactc aggcgacgct caccctgacc
    12001 ttgccagcgg cggtggagtt tgacgtggtg cgcctgcgcg agaacattcg gctggggcac
    12061 cgcgtagacg ggttcgtcgt ggaggtgtgg cagggtcagg catggaagga atacgccaaa
    12121 gggcagagta tcggtgcctg ccgtctgatc cgcggctcca aggtgcagac ggaccgtgtt
    12181 cgtttgcgta tcacctcggc ggcggcctgc ccctgtatca gtgactttgg cctcttcgcc
    12241 ctgcccgcga cccttgcgcc ttcacttccg aagccatgaa aaaaacgcct gcccggttac
    12301 gccccgtttc gtttgaacgc cgcttcaccc gccatgccgc agggtcggtc ctgatccgtc
    12361 agggcgacac ttgggtgctc tgtaccgcga gtgtggagga gaaggtgccc ccgtttctgc
    12421 gcggcagtgg caagggctgg gtcaccgcag agtacagcat gctgcccggc agcacgatcg
    12481 agcgcaagga gcgcgaaatc cggcgcggca aacccgatgc gcgcggcacg gagatcagtc
    12541 ggctcatcgg tcgcgcactc cgcgcggccg tggatttgga gcgcctcggc gaacgcacca
    12601 tcacgatcga ttgcgacgtg cttcaggcgg acggcggcac gcgctgcgct tccgtcaccg
    12661 gcgggatggt ggcgttggcc gatgcattga cggcgctggt gcggcagggc aagctctcct
    12721 ccccgccgct ccggcatttc gtggcagcgg tcagcgtcgg catctgcggc ggcaagcccg
    12781 tgcttgacct cgattatgcg cacgacagcg ttgcggaggt cgacctcaat gtggtcatga
    12841 ccgacgacgg gcggtatgtg gaggtgcagg gtactgccga gggcgagccg tttgacgagg
    12901 ccgtgctgga cacgctccgc tctcgggcgc gcaaagggat ccgcgatttg atccgcatgc
    12961 agaaggccgc gttgcgtgag gagcccccgc accatccgta acgtttttct ttctttccga
    13021 tgaaccgtgg caaagacgct ctcgtttgaa agaggtggaa acgtcaccca tcaaacgagg
    13081 agatccgatc ttgctgttcc gaaccatcct gttttgctgc gcgttcgccc tgttggccgt
    13141 ctgccgcggc caaaccaaca cgccagtcga tctgggaacc tttgttgtgg agggcacccc
    13201 gatatcgaag taccgggcgg aaaccgtttc gaccgccacc ttcgctgacg cgaaaccgga
    13261 agagctgccg cagacggtgg aggtgctgac ggaagacttc atcagcgaga tgaatccggt
    13321 ggatctgcac gatatgctgc gccatcaggc cggtgtctat actggcggca agtccatgct
    13381 ggaccgcact tccggacaat acacgctgcg cggcatgtcc ggcagcgacg tcatgctcaa
    13441 cggcacgctc ggcctggccg ggcccatggg cacattcatg gacccttcgg ccttcgaacg
    13501 tatcgaggtc gtcaaggggc cggtcggttc gacggtcggc ggcgccagct ccacactcgg
    13561 gccctatggc gcgggcggct cggtgaattt ggtcatgaaa caaccgcgtg acgaccaaga
    13621 ctttctccat ctgaaacccc gcgcctccct cggcgagcac agtcagcgtt accgcctccc
    13681 gttcgatatc aacgagcgcg tgattgaaga ccgcctgacc gtgcgcctgc ccggcaatat
    13741 cgagtacggc cgtccgttct ggctctccag cggctatgac tggcgcgaat cgtactatct
    13801 cgcgccctct gtgttcatgg cggtgcgcga tgatctgcgc ataggcgcca atctctcgat
    13861 ccagtacacc gaccagcccg cttatcaggg cataccggtc tacaagggca agccttacgc
    13921 cggatatgac tgggattcca atcccgcgct gtcgcacatg cgcgacacct acatcggcca
    13981 tacccttgat gcctatgtgg agtgggatgc ttcgcgcgtc tggaccttgc gcagtggcgc
    14041 tggcgtcgcg caggccgata ttgagtttga gcatctcggc cccgccgcgt tcgcgaatgc
    14101 ctccaccgac gcgttgctgc cgctgccgct cgccaagccc tatgatcatc aggagggtga
    14161 catgctgtac cgccgctaca acgcctacca gcgcgccacg gcggttttcg ataccggccc
    14221 ggtcgggcac caggcggttt ttcagggtga tttctcgcgc aaaacatcta aaggccgcag
    14281 ttatttcgaa tctgtcgcga agcccgatgc cgtacaccgc tgggtggcgg ctgactatca
    14341 gaataccgag ctggacaaat acggtttcct cgcgcaggat tatctctcct ggtggaaatt
    14401 acgtgtgctg gtcggcggcc gttttgatga tcacgaaagc gaccaaggca actccgccaa
    14461 cgctttctgc ccgcgtggcg gcatctcgtt cctgccgacc gactggctgg ttttcttcgc
    14521 aaacgtctcg tacacggaag cgccgaactt cggttacaac ggtaaagaca accgcgagct
    14581 gacctccaca tgggaggcta tccagtacga gaccggtttc cgcgtcgcgc cggttaagac
    14641 cctttggctc tctgccgcgc tctaccgcat cgaccaggat aacaccccca tcgccctgcc
    14701 tgaagaccgc acccgctaca ccgaagaggg ggccaacgaa tcgcagggcg tggaggtctc
    14761 gctcaccggt aacataagcg acaggtggtc ggtttatgcc tcatacgcct acaacgaata
    14821 cgagaacaaa cagcagaaaa aacgcttcga ccgctacccg ccgcacgcgg cgacgctgtc
    14881 cacgtcctac cgtctggctg gcgggccgct agatgacatc gtgctgggct tcggttaccg
    14941 ttaccgccac cgctacgccg ccaccatgcg cggcgagtat gtcggcgaca atttctatat
    15001 agacgactcg cacgtcttcg actgctcggc ggatatcccc ctgtacaaat tcggcgggcc
    15061 gaaaaatgtg acgctatccc tcgcctgtaa aaacatcttc aacaggcact acattgaatc
    15121 cacccgacac tattaccaat gttttcccgg agacccccgc acattcgagt tggcgcttca
    15181 agcggaattc tgagcctgca tttcggtcaa ccgaacgttt tgcgcgatca ggctcgcgca
    15241 tgattcgggt gatgcgtgaa acccggaacc aaagaaggcc caaaatacct gtcggttttc
    15301 ccgctcggca gatcccggac tctagcggag cacagcgatc ccatagagtc gcgcgccctg
    15361 atccggcggc gcttgaatgc gcacagtcag attcgccgtg gcgcgtaacg cctccggcac
    15421 gtcaacggtg acgactcctg cggcgtgcgc ttgtttcgaa gccgggtgcc agactgtgcc
    15481 gtcagccgaa aacgttacgc tcaccttggc tccgcaggcg aggcgcaggc gcgtggcatc
    15541 cttcccccgc aacgtcaacg gcaggcgata ggtcagggcc gcccctttcc ggaattggaa
    15601 attcgagatg cggtccgtca cccccgtcgt gtcgagacgt aaaggcagat cttcctcgcc
    15661 gaccataaag acgcggtgcg cgccttcagc caatgcggtt tcaatcaggg tccgggtggg
    15721 gatgttgcgg tctgatgcca cggcctcgaa ccgcagcggc acgccctcct tggttagcca
    15781 acgcaaccct gaacccacga cacgtccgtt cgacacgaag acaagtcggt acgcaccctt
    15841 gtccggggtc caggccccga cgggtgtaat ggtctcgact acgcgaacgg gtttgccgcc
    15901 gaggcggatt tcgcactcgg cattaccgtg gttgtgtccc gcgatattcc agatctcgcc
    15961 gggaaacgcg gccagaagcg gggcaagctg tttgcggata cggtggcaat ccggatcctt
    16021 gagcgcttcg tgcgtgaaga gcaccagagt cttcgcattg gcggcggcct ccagatcgcg
    16081 cttcaaccag acgagttgcg aatcatccag caggatgccg gcatggttgg tgttctccgg
    16141 tttccatgtg ttgtatccgt gcaggccgac gaaatgtacg ccttgcctgt caaacgacca
    16201 gtagagcgga cgatccggcc agaccgcgtg ataggcttcg ggcgagcagt cgttgttgcc
    16261 gatgatcatg tggcagggca cggtgagacg tgagacgatc agatcccgca cgcgccggaa
    16321 accggcttcg gcctgcggcc aggtggcagg gttacgtgtg gtcgcggcga tcagatctcc
    16381 ggtcagcgca acgaacgcag gcgcgggccg cagcgcgttc atgtcatccg cgaagttgcg
    16441 gcctccgaat ttatccggca ttttcaaagc accggatgtg atctcactcg catgcagatc
    16501 tgtggcttgg agaaaaaaga aacttcccgg cgcgttcgga tcccacccta gggcccggat
    16561 cagttccgcc agtcctgccg cctctccctc tgcaaacgta cgtccgcaga tcagtgcggc
    16621 acccgcgccc gtcccgccgg aaagaaactt ccggcgcgtg attcctctga ctcgcatcgc
    16681 tcttctccct aaccccgctc agatttgtca gacaggatgg tcttgattcg gagcatacca
    16741 tgttccgatc cgatgtgtcc atgcgcggat gaaaccaagt tgcgggcgcg ggcacccgcg
    16801 tttccgacgc cctcccgtct tgcttgcatg aaggttcatg tgtttcattt ttttcgcgtc
    16861 tccgttctta cgttgaagcg tgtgcatgaa cggtgttatg cttacgggta acgattcgga
    16921 tgaaagcgtt gatccaaaga gtcgctgagg cttcggtgga agtcgaggga cggcgtgtgg
    16981 ccgcaatcgg ccgcggcctg ctggtgctgc ttggggtaac gcatggcgac acgctcaagg
    17041 aggtcgcgtg gctggcacgc aagattgcgg cgctgcgcat ctttgaggat gagcagggcc
    17101 tgatgaaccg gtctgtctcg gagacgggcg gtgcggtgat cgtggtctcg cagttcacgt
    17161 tgtatgccga cacgcgcaaa ggcaatcgcc cgagtttcac ggaggccgcg cggccggagc
    17221 aggccgagcc gctgtaccgc gaattggtgc ggcaactccg cctgactctg ggcgcggacc
    17281 gcgttggaac gggtgtgttc ggcgcggaga tgaaagtgcg tctgttgaat gacgggccgg
    17341 tgaccgtcga gttgtgcacg gaggttgcgt gtccccctcc tcgcacatcg tgtggctaac
    17401 gtcagttctt tttgcgattc cctcgcaagg ggttcgatcc cggccccgat ttcgatatcc
    17461 gcttgtctgc ggcgctcaca cgcgccagcg tttgtgtgcc cagaggtatt gttccgggta
    17521 gcgccggatc acttcgccca gtcgcgcatt gatctctgcg gtaagtgcgc ggacatccgc
    17581 ctcccggttc tcggtgggct gaaagcggat cgggagcccg gtgaccatcc ggtagcggaa
    17641 gggcccctcg cgcatgaatc cgccgaccag gattggcgca ccggtttgga gatggaggcg
    17701 cgcgggtgag gtgtgggtgg aggcgggacg gcccagaaag tcaaccgtca cgccctgctt
    17761 ggggcccgcg tgctggtcca tcacgagtgt catggcagcc ccttgatttt tccacgcgcg
    17821 caacacatcc ggcgagaacc cctttttttt ggggatgatg gtgatctgcc cgcggaagtg
    17881 gtgccgcttg aggaaacgtt cgacgaacgg gttgtttagg gtgcgggcca cggcgatcat
    17941 cgggcgggca cgggagagga tggggacagc cgactcccag gcgccgaggt ggcccgtgag
    18001 gatgagaatc ggcacgccgg gcgattccag caggagggcg aacgattcgg cggggccgtc
    18061 gagcgtgatg tggtcgcgcc aggtctcgtc cgaaatgacg tttccggcct tgagggactc
    18121 gcacaggtgg cccgcaagat ggccgaaggc ctgacgcgca atccggtcgg cttcgtccgg
    18181 gtcgtcggtg atatgcgcct tcaggatgtt ctcgacagcc gtgcgtcggc gtgcggcaaa
    18241 cagcgggtag gcgagggcag tcaggcgcgc acccagatca taggcccgcg atgcggggac
    18301 caagccgtag aacatgccca gaaagcggat ggcgagatat tcaagataag cggcgaattt
    18361 tttgagcagg ctcatgcggc acggattagt tggcgaggtc acggagcagt tgctggctga
    18421 ggggtttgag ttcccggaga atgtgccggg gcagatgggc ttcctgctcc agcagaccgg
    18481 agatctgttc ggcggcctcc cggaggttcc gttgcgccat tcggatacgg atcagcgtca
    18541 ggcgagcgga gaagtcatcg ggtttcgcct gaagcacggc atcgagggcc cgggaggcct
    18601 catcggcgcg tccggtggcc aacagcagac gggccaatgc gtgccaggcg tcaaggcgct
    18661 gcggcgcgtc gtcgagtgca cggcgcaaaa acttttcggc aagggcgtgc tcgccttttt
    18721 ccagcaggac cgtgccaagg cccgcgaacg ccggcgcatt gcgttcagtt tccaaactcc
    18781 ggcggaagag gtcttcggcg aggtccactt cgccgcgttc gagccgcacg cggccccaga
    18841 ggtagttggc gaaggcgtgg tcgggattct ggcgcagaat cgcgcaggcg tcctgttcac
    18901 tgaaagcggg cacctccagc acgtcatcaa gccgcaggat ctcctcgcgc acgtcgttga
    18961 ggttagggtt caactccaag gctcgcagga agttcgcgcg tgcggcgcgg tagtgttccg
    19021 taccgttgtg ccggagcaga taaccgcgga ctttgtacat gagatagtgt tcttttttaa
    19081 cggaggcgct gcgcaacacg gggaagacgc gcatctcgag ttcgggatac tcctccagtt
    19141 gcagcagaat ctccgccagc agaccccaca atccgatgtc ggcgggtgcg gcgttgagcg
    19201 cggtgttaag ccgttcgcgg gcttcgggca gtttgccgtc gagaatcaga agggccgctt
    19261 cgtgccagat cagttcggcg ggagtgacgc cgttttcctt ggccagatcc atccagagcc
    19321 cggcctcggg aaggtcacgg cgttcgatgg ccaagcgtgc cttgttcacc agcgcgaacc
    19381 ggtcccggtc atcgatatcc agaagcaggt tgagttggag gtccgctatg tcaaactcat
    19441 acgtctccag atgtttggcg attgtctggt agagttcgcg cttcttgtcg cgtacggcca
    19501 gaaaggggtc cggctgccca ggcgagaggg tgtgcgacaa ctgctgcagc agtccggact
    19561 tcgtccaata gctcttgcgg acctgctcca gaatatccgg agagatcagc gttccggttt
    19621 gggcctggac cgaggcgggg gtgaggaggt gggtacgggc gtaccgccgc gggatctcgc
    19681 gcagacgtgt ctgcagggcg gggagcgtat ccgcgcgcag gccatggccg agggccagat
    19741 ggtagcggtt cagcagtacg ctgaggttgt cgggcatcag cgcttcggtc tgcgcgagca
    19801 actccgcagc ctcctcgaag cgcttctgcc cggcgaacag aaccgccagc tcattgccag
    19861 cgcaggccag ttgtccggcg agcgccgcgc ggatcttggc gaacaggcgg atgctgtccg
    19921 gggttgcggg gaacaggtgc ggttgcagga actctacgaa agcttggtgc cggtcgcgca
    19981 gttgggcgag gtctacggac acgtcttgcg gcagaccgtt gaagaaaatg ctgttgggca
    20041 ggggcgtgaa accgttctcg cgccagaaga ccggagtgcg gaacagggca atgcgggagt
    20101 aggcgttggt ttcctgggta agccattcgc gcaggaaaga aacgggcgaa aggtcggccg
    20161 cgttcagaag ccgggcttgg attctggtaa gggagggatc ctcccggatg gcggtgccga
    20221 ggtgcttcgc gttggcgccg tccgggctct cttccgtttc gatgagccgt agccgacgcc
    20281 cgtcctgaaa ggcgcggagc atcaggtgat gcgtgagagt gctgctgttg acgatccagt
    20341 cacaggtgtc cagcgttgcg tagatgcggt ccgtgacgac atcgacgaag gcgccttcac
    20401 gcggatcgat gtccgtgaaa ctgcggaacg gcaccagaca cgcgagtgcc aacaggggcc
    20461 agcagagcca tcggccgata cggcaaacga gaggatggtc gcggtattcg taataatcga
    20521 ggtcctcgga caactgttcg tcgaacatct cgcgcatcag ataccagacg gcaatcaaca
    20581 ggccggtcag gaaagcgacg attgtggaac tgaagaccgg aatgcgggag gtcatacgcg
    20641 caatcgccca gggcgacatg cgcaggttga gcaggctggg gatgagtgtg gcggccagcg
    20701 tgagctgcat gaggaacagc agcggtgtgc gcttgcggaa ggaaaaggcg aaaatgaaca
    20761 gcgcgatcgc gcaggggaag agcagttgca tgaagatgta gctccagccg aagctgggca
    20821 cccagcgggc aagttcgtcc agcatggtcg cggtcaggac gttcaggatc gggcggggtg
    20881 ccgggatggg gaccgcggcg cataaggcgc tggcacgcca taggagcatg ccgcccgtca
    20941 gaaacccaat cagaccggtc aacaccacgg ctagtgtgcg gtaaaaagtg gcgtgttcat
    21001 tcagcaggag cgatcgcagc agaaatccgc tccctaccgg aaggagcaac agaaagagag
    21061 gggactccac gcagcaggcc gccagcagaa aagaggaggc gaacaaaaag aaagggtggg
    21121 ctttctggtc ataggcgatc agtagattca gaagcaggaa gaacagggcg agatcaaagg
    21181 cgaagggata gagacgggtc gcggcgatcc agaacggtcc gcagaaggcg aaggcgaggg
    21241 cggcacccat gccgccgagg gccgcggcgc gcgcactgcg ccagttatgg gcctgaaccg
    21301 aggcgggaag gatcggcatc tcgctggcgg gtagcgggaa ggacatgcga ttgcggttcg
    21361 ggagatcgcc ctcgtcttcc cgtatgcctt ccgtgccggg aggcagggcc gccatactgc
    21421 cgccgggatt ctcagcggcc caaatgaaaa tcagccgtgc ggtcagcaga taaaagaggg
    21481 tcgcggccaa cgtgccgcag gcggcgcaga agagattgag ccgcacgggc agggtggcgt
    21541 acggcaactg cgcgatcggt cggatgatca gactgaacaa cggatgcgcc agatcgtttt
    21601 gctggcagag accggcagcc attgcagtca ggaaggccgg ggttccggga tagaggtgac
    21661 ggaaaagcgt caccgcatac actacggccg tcagcagcgt cacgaccggc aagaaaaagc
    21721 ggatgagctg ccaaggggtt gtgaaaaacc tgtgtttgcg ggacgccatg gccttgcctc
    21781 actggtgcgc tctacgacgc cacgcgcggt caccgtacac gccagcgcgg gagaaatcaa
    21841 acggacggaa gccgatctgt tccgcaggcg aaccgcgccg cagacggaag tcgcgttccg
    21901 cagcgttttc aaacagcgga tccgccacga gcccgtccgt gtcgcgcccc atggcctgcc
    21961 attcggcgaa ggtcatgcct ttaaaatcag ctggcccgtc cgtccgccac cacaggttgc
    22021 tctgccagtc cactttggcc tggatggagc gggtataacg ggtgtcgctg aagtttgggg
    22081 cgttttccca gatgatgatg ttgcgctcga cggtcagcga gcggtgcggt tctacccggg
    22141 tgaccgccac ctggcactcg cggctgaagg cgagaatgtt gttgcgcaac acgttgtcac
    22201 gaccgtaatg ctggtggaac gatccgtcct tggtgtcgta cacgagattg ttttctaaga
    22261 cgatgccctc cgaaccctcg tccggataga gtccccatcc gccgtacgtg taggagtcga
    22321 cgttaaagat cacgttgttg ctgatgcagg tgccgaaact ggtccccagc gtgtagacgc
    22381 cgcccatatc gcccagcatg cgctgcccga tcgtgtggat acggttaaag gcgatggtgt
    22441 tgcgttgtgc cgcgctgccg cggtaccccc agacccagcc cacagagacg cccgtgtaga
    22501 aatggtcgct gatctcgcag tgggtgacgc tgttgtcggg gctgtggccg atccagacgg
    22561 cggtgccgct cgcgtggaac cgtccgccgt gagtgatgat gcagttgtcg accgtgttga
    22621 acgcggtgct gaaggatgtc agcgcctgaa ctgcggcatt ggtgctggcg ctcactccct
    22681 tggctccggg atcgccgatg cggatgccgc ccgcgcccag atcggtgagt gcgcactgct
    22741 ccacccggtt gctgacacag cccgcgcgga accagaggcc gtattcaccg gtgtgcgcga
    22801 tgtcgcaacg acggaaggtc accccatgcg cgccgtccgc catgatcgcc gcttcggtgt
    22861 gcgcggccgc ttggtggggt tcgaactggg tagggccggg cgcgttcagg ggaccgcaga
    22921 tttcgtcggg tagaaacgcg caggcgatct ggttgaggcg gcgcggcgaa tcggtataga
    22981 ggaagccgag gttttcaaag gtgaggtgcc gtacgaagac accggcgacc ggatcgccct
    23041 gaaagacaac cagcgtcttg acccccgata agggagccac aacctctgcg cgttcgggcc
    23101 gctcgtccgg caatggcagg tagtggagtt tgcggttgac gccgtcatag aaccactcgc
    23161 ccggcgcatc gagtgcggaa cggacgttct cgacatagta aagggagttg gtctgccact
    23221 tgttccaatg cttccaaggc gcgccctggg tgatgagcgt cgcctttccg acatcgaacc
    23281 cggcgatcat ccggcgcgtg gtgtcccatt tgtggtggac caccacttgg gcgaaacgcc
    23341 attcaggctg cggactgcgc gccaagggat cgagttcacc ggcacggccg gtcagggtgg
    23401 cccgggcgta gaccccgcgc ggagaggcgt tggtcagaat cgtctgttgc acagcggcgg
    23461 gattgaagaa cccgcggttg ggatgccgcg cgcgcggggc acgccgaccg ttgacgaaga
    23521 gggtctcaaa ataggcgggt gtgccgctgg cgtcgcacgg gatatccgcc agccaccggc
    23581 cgtccggctg cacctgccag ccggtgatca cacggccgcc gcacaggcgt gaccggtccg
    23641 caccccggcc gcggtaggtg accggcgcgt cggcggtccc ggagtcctcg ggactgaaca
    23701 cgatcggttc gttcacaggg taaaaacccg tatcgacatg cacggtccac gcctctgaac
    23761 aaccagcctt ccgcgccgcg cgaatcgcgt cgcgcgcctg cgtcagcgaa gtgaacgggc
    23821 ggcgtcgcga gccgtccggc ggttcagagg ttttcgcggc gggagcgacg tacaggtcag
    23881 cggcgccgag tccggaggcg agcaggcaac agaacagcag cagtgttttc atagggtgtc
    23941 acgcatcctg gggttaaagg attaacggaa agaggcgagg tcgcatttgt ccatgtcgtc
    24001 gaactcctga ttctcaccgc ccatggccca gacaaaagcg tacccgccgg tgcccgcgcc
    24061 gcaatggacc gaccacgcgg gtgagagtac gacttgcttt tcacgcagca tcacatgccg
    24121 cgtctcatcc ggcgcgccga gcagatgcag gaccgcaccg ttgggcggaa gatcgaagta
    24181 acagtacacc tcggtgcggc ggtcgtgcgt gtggggcggg aaggagttcc acacgcttcc
    24241 ctcgtgcaac atggtgaacc ccatgacgag ctgacagctc cggataaatc ccggccggat
    24301 gtactgatag atcgtgcggc ggttcgactc cgcaagtgtg ccgagttcga tgcggttggc
    24361 atccttgagc cccgctttca cggtcggata atcggtgtgc gcgggatagg aaaccagata
    24421 gaaacacgcc ggtttctggg ggttggcgct agagaagagg aggccgcggc tgccgcgccg
    24481 gacgtagagg cattcgcgcc gcgccaaggc gtgctcggta ccgtccacca ccacggttcc
    24541 gggctcgccg agattgatga tcccgacctc gcgccgctcg cagaaatagg agcaggccat
    24601 ttcgcgtccc cccgtcagtt ccagatgctc ggtttcggga acgatcccgc cgaccaccgc
    24661 gcggtcggtg ttcgtgtaga cgaggttgac ttggccgggc tggaacagat cttcgattac
    24721 gaagcggttg cgcaactcgt ccgtggtcat acggcgatag gattgacggt caggtgtttc
    24781 gatgtttctc atgatgtctc ccaatctacg tttctgaatg tcggaaccac tatagcagag
    24841 gtcagccccc cggtccaata cccgatttgc cgggatgcac cgcgaaaaag cggggtcgca
    24901 ccgtgcacgg acctcattgc gtgaagggtt caatgacgcg ttcaagaatc ccgtgcaggt
    24961 gtgcgatcag cacaccgtaa ttgacgatcg gcacgcctgc ggccttggcc tgatggatgc
    25021 ggtagcgcat ttcacggcgg ttgagcatac aggcgccgca atggacgatg agcgcgtaag
    25081 gggaaaggtc gctcggatag ttcatgccgc tcgtgtgttc gtaaaccagt ttgcggccgg
    25141 tgtgccggag cagccaacgc gggatcttga ctgtgccgat atcgtcgcac tgccggtggt
    25201 gtgtgcatcc ttccgccaca agcacgcggt cgccgtcctt caacgacgcg atcgcgcgcg
    25261 taccgcgcac ctgctccgcc aaatcaccct tatagcgcga gaaaagaatc gagaaagagg
    25321 tcagcggcac gttgcgcggg atttcggccg ccacgctctc aaaaacctgt gaatcggtaa
    25381 tgaccagtcg cggaggccgg gcgagcgtgg cgagcgcggc gcgcagctcg aattcgcgcg
    25441 tgaccagcgc cagcgcgcca gcctcaagga tgtcgcgcac ggtttgctgt tgcggcagga
    25501 tcaggcggcc tttgggcgcg gcggagtcga ttggtgtgac cagcacaacg aggtcgtttg
    25561 gtttcagcag gtcaccgacc agccgttccg catcgtcttc cggcgcgact ttggccagtg
    25621 ccagcttgag atcctctatg cccgcgcgtg taacggagga gaccgtgagt agcggcgtct
    25681 tgcactcctc ggcggcctga cgcagtgccg agggcgaagg cggccggagg tcgactttgt
    25741 tgaagaccgt caacagcgga atacccttgg cgcgtaccag atcgaggacc tcgcgctcgc
    25801 agtcgccgat accagagtct gcctccagca ccagaatcgc cacgtcggtc ttgttcagca
    25861 cgtcacgcgt acgctcgatc cgtagtgcgc ccagctcgct ctcgtcgtcc aaaccgggcg
    25921 tatcgaagat caggatcggg ccgagcggca ggagttccat cgccttgcgg accggatcgg
    25981 tggtggtgcc tttgaccgct gaaacgatgg agacctcctg accggtgagc gcgttgatca
    26041 gactggattt gccgacattg cgccgaccga agagcgcaat gtgcacacgc tcgccctgag
    26101 ggatatcgtt gaggctcatt gttgtatatc tgccagcgtt acagcacgcg gcggtccctt
    26161 cttgaatacg gtcattttac aggggcacga tccggattgt caacgagagg ctgcctttcc
    26221 ctctggcctc gcgcgttttc ctgattgact cttttgccct ccttgtataa taggtgccga
    26281 ctataggaac cggtgtgttc ccggtctgcc ctatgggatg gcagggattc gtgatatgag
    26341 aaaaatacgc tttcttgttt gggtcatggg ggcgtccttg gccttcgggc gtggcgaaag
    26401 ctggatgccc aagccgcccg caccgccttc ggtagcgacg ctgacaaatg aggcgcggta
    26461 cgtcgagtgg aagtttcgcg gggacgtgac agatccgtcg acgggccttg ccgcgcacgc
    26521 gcttcgcacg aacgcgctcg cgctggcgtc cgggctcgag ccgaaggagg attggcgcgt
    26581 cgtgaaggcg aaggtctacg cgatgctctg cgaccagatg gcgatcgacg tttcgccgct
    26641 cgactggtat ccggcgattt caatctggaa tcgatacgac cgcccgatgt ccgctgtggt
    26701 ctggcggcgc aacggggaga tcgcgcgcaa gtgctacccc gacatcaccg ggcggatcaa
    26761 caaaggcaac cgtgacggga tttggcgtgt ctggaaggac tttgaccact ctgttcccgt
    26821 ctgggaggat atgttcaagc tcggcttcgc cggaatgcgc gaccgcgtgg acgcgcacga
    26881 tgacgggacg cccttttacc gctcgctccg catcacggcg gacgcatcga tcagactgat
    26941 cgaacgtttt gccgcgcagg cgcgcaaacg gctcggtacg gcggacggaa aaagctcccg
    27001 cctcgtaaaa cagatcgccg ccttggatca gcttgctaaa ggtccgccgc agacggctta
    27061 tgaggtgctc ctgttcattg atctctattt tttcatgtgt gagcatctcg acgccatgca
    27121 gtgtcgttcg ctttcgatta tcgacaggaa cctgtggccg tactatcagg ctgatcttgc
    27181 ggcgggacgg acgaccgaag ctgaatttcg cgagcaattc aggcacttcc tctggcaatg
    27241 ggggtcggtt gacaactact ggggccagcc cgtgacgatg ggcggcacaa aagcggacgg
    27301 cacgacggag tacaatccgc tttcgcggat cattctcgat gtgatggacg agtgcgccct
    27361 cccgacgccg aagttccatg tgaagatcgg caagtcgacg ccgcgagaga tcctcgacaa
    27421 gctcctcagc atggcgcgac gcaaccgttc gctcacgttc atcggcgaag agtcggcgcg
    27481 gcgcattctc gaacattatg gtgccaccaa agaagaggcg cgcacgttct atacgaccgg
    27541 atgctacgag atcgtgctcg cgaacggcgc gaataagact ggtgttggct acatcaacct
    27601 gctcaaacag gtcgagttcg tcctgcgcga cgcgaaaggc ggaaagttct ccgcagccga
    27661 ttatgcggca ttccaggcgg aatgtatccg ccgtatggtg aacgtctgta cgaactgtct
    27721 cgcgatcacg agcgcgttcg agcagcatct ggatgacgtc aaccccgcga acatcacgac
    27781 gctcggtgtc gcgtacgcgg tcaaaacggg acgtgatgct tttgcgaatg gctcggaacg
    27841 cggaaacaac actgcgatcc tgatcgctgc gatgggcacg accgtcgacg cgcttgttgc
    27901 ggtaaaagat cttgtctacg aaaagaaaga gatgtccctc gcggacctcg gcaaactcat
    27961 ggctgcgaac tgggagggac gtgacgacct gcgtcttcgg atgaaacgct cgaaaaggaa
    28021 gtggggcaac aacgatccgg acgcgaacgc gacggcggct gcggtctcaa aggcactcgc
    28081 cgcggcggtc aacggcaagc cgaacgcgcg cggcggcatt tacgttctct ccggacacag
    28141 cgcccgcgcg tttatctacg agggtgcggc aacaggcgcg acgccggacg gacgcaaggc
    28201 gggcgaagag ttctccaaga acatgtccgc gacaatgggc gcggataccg aaggcgtgac
    28261 ggcgctcatc cagaccctcg gcgcactcga ttcccgcgat tggccgggcg actttccgct
    28321 cgacgtgatg ctccatcccg cgaccgtgga aggcgaccgt ggtctcgcgc tgatgcggac
    28381 gctgcttgat gtctactatg cgaacggcgg gcttcagatt caatttgtcg ttgtctcggc
    28441 ggatgaactc cgcgatgccc agaagcatcc ggagaaatat gagaacctgc agattcgcgt
    28501 gtgcggctgg aacgtacgat ggaatgatat ccccaaagcc gaacaggatg cctacatcct
    28561 gcgggcggaa agcatcatgc aatgaacaga agggaattca tcggatcttc cgcggctttt
    28621 gctgtggcgc cgcttttcgc cgcaggcgga gggaaagtca gaacgccgct catgctcgat
    28681 acgaagcgga tggcgatctg ggacggcccc ggcgtgcgga ccacgttctt cgtgaagggg
    28741 tgtccgctca agtgcatctg gtgtcataac cccgaatcga tttcggccca acgccagacc
    28801 gcgcgattcc agcatctctg ccgacgttgt gaaaagtgta caatggacga agcgacctgt
    28861 ccgacccgcg cgctcaagtt ctacggcaag ccgatgaaga tcgacgagat cgtcgcaaag
    28921 gcgcttgagg ataagccgtt ttacgattcg tcgggcggtg gcgtgaccct ttctggcggc
    28981 gagccgctcc ttttttggga atggctggcc gagcttttca cggcattcaa gaaggcgggg
    29041 cttcacacct gcctcgacac ctcgctttat gcgccgcccg tggcgatcga ggcaatgctc
    29101 ccgcttgtcg acatgtggct tcccgactac aaagccgatg acgatgcgcg ccatcggaag
    29161 tacacaggcg ttccgaacaa aatcatccgg aaaaaccttg agcgactcgt ggcggcgaag
    29221 gcgaaactcg aagtgcggct tcttgttgtg cctaggtgta cggatgatga tgatcttgcg
    29281 gcgcgtcacg cgtatcttga ttcgctcgac attccgcgcg cggcgcaggt cgaactcgaa
    29341 tatcgtgatc tcgcccgctc gaaatatctt gcgctcggca tgccggacac gatgccccct
    29401 tcaacgtaag gggcggggag gcggatcaaa cgccgcaagt cgaatgtcgc gggtcgcaag
    29461 tcgacgacgg gtacgagtac gaggacggga aaacgatccc gatagtggta aacccggaac
    29521 aaaccgagtt aagaaattca ttggacgggt tgccgcaaca cgatgtattc tgttttcgtc
    29581 ggcttcgggg gtgtgccctg aagcaacagg gatatccatt ttatgcgtct acatgaaaac
    29641 tgccgtatga acaacttcaa attgggggtc gtggctctcg ggggagtgat ggcgggaacg
    29701 atactggcgc gtcccgtgct ccagcccgag gtgaaggaat acgtttcgtc aaaaggcgtt
    29761 gcggactatg cggcggcgcc ggttgtctat caggaccaga gacagtgccg gattggtgcg
    29821 gacgagattc cgtcgacagg tgaaaagacg gcctatgccg ggcatgcgct accggaggca
    29881 ggcgtattca tcgctattgc cgattcggcg ctcggcaaga agttggtcgc ggatttcagg
    29941 ctggacgtgc cggcgaagaa acagggctac gcgatccgcg tcgacgggaa atgcgcggtc
    30001 atcgtcgggc acgacgccat cggtgcgctc tatggtgcgg tgacgtttcg gcagatggcg
    30061 gcgaagggag gcagggttga gtgcgcgatg gtgcgcgact ggccagacgt gctctatcgc
    30121 ggtggagctt cccttgggcg tgggctatgg ccgtacggga agtccgacgg tgttgacggc
    30181 atcaaggcgg cgctagacgt gatgatgcgc cacaagctga atatcatcag cgactttttc
    30241 ggactcgatg agttggcgcc tcgcgagaaa ctcgcgactt ggcgcgaact tgtgcgatac
    30301 gcgcgggacc gcggcatcta ttcctattac ttcatctcca cggcgctttt tcgccgcacc
    30361 aacaagcctg agggcatgac cctcgagaag tggccgtgcg tcaaggacct ccgtacctgg
    30421 aacgaatggt attactgctg gtccgacgac gagggaaccg agaaggccgt caaccactgc
    30481 gcagacatcc tgcagaacat cggcgcggag gacgggattg ttcaaatcca tcccgtcgac
    30541 ggaggaggtt ggaaggatcc cgagttctgg agcaagcgtt gcgcgaaatg ccgcagtctc
    30601 tggaatgacc acgagcggtg gaaggcgacg gtgcaccagc tagagatttg gaaccgtgtg
    30661 ctgcggcgga gatgtccgaa tgtgatcgtc ggttcctgca tctatccgta cacatacagt
    30721 gcgctgctga caccggagaa ggaccgcacc gacctctgga gagagtcaca tacggaatac
    30781 tggtcaaagg tcagcgaagg gatcggaaac aaggagttct ttttcagcag ttggatgtct
    30841 ccgccatggc tggccgaaca gattcggcga tatgtgccat cgcgtcctat tcatttcgcc
    30901 cagtatccgc agttctcagg catcttccag acaacttcgc gcaaggcatg ctccgtgtac
    30961 gaggagaatg ccgataacac ctatcttcct cagggaaccg acttcgacgc ggtccaatgg
    31021 gaatctctac tgttgggtgt cgaatccaca tggaaccgaa acatgcccgg ggcggaggcc
    31081 tttgacggcg gaacatatta tgacgcggcg gttgaccata cggggccgag gatcgtgatg
    31141 gaacaggtgc tgccgcgcct ctgtgcggct ttttggggaa cggaactcgc gccgtacatg
    31201 acgcgtattc tgtcgtcggg cgtgttgccg tcctacatcc gggatccggg caaggcgctt
    31261 gcctactgga attccgttcg gaaagatccg ctcttcgatc cgacggtaga acaggtctcg
    31321 actctggaac ggaagcgcat cgagaaaatc gtggacacgt ccgagcggat gcgtgagcag
    31381 gtgaaggctg cggaaaccgt cattcgagac ctgacggagg cccgcggtca tctggacggg
    31441 ttggactcgg tccgtcgccg gtatttcatg aagctggcca agtatgcccc gttctggctg
    31501 gccaccgctc gcgcccgata tgccttgcgc gcggcgaatg acctgattgc cgccggtcgg
    31561 catgaggagg cgaagagggc cctgccggag gggctgcggc aactgcggga ggagtggcgt
    31621 caggcggagg agaacctcaa ggctctatcc agtgaggttg actgctctcc ccgaaggtac
    31681 gtatggccgg tcaagctgga gacgttggtt gctgaattcg agcgtgcgga aacctcagcg
    31741 gggcgtccat agttgcgtgc catcagcgtg gagccggacc gccgaaaccg tgccgggcct
    31801 tcgcggcagg gatgtctctc cgaggagacg cccgagtacg gggacgaaaa aacgatagcg
    31861 atcctgatcg tgattacgat ggggcgcgcg tcccactcaa aaattttgat gcgcatggct
    31921 ttcgcgcgag gctgtacgaa gggagtcaaa aacgctacta tccggttact gaatcagtac
    31981 ggcgcaatgc agccgttgca caaaccggag ggtacgatgg cttcacgacg gatgtttttt
    32041 gctctgatca ttctgttgag cgcgatcccg gaccgcactg cgcgggcggt ttcagcggag
    32101 tggacattgg aaacggttga ccggaatgcg cgcggggtcg ccgattgggc gcgccccaat
    32161 cccaagcgga gtctcgtctg ggaaaacggg acgctgtacg cggggatcac ggcgcttgga
    32221 ctcgctgatc cgtccttgcc ataccttgat cagatccggc agcttggtgc gcatcatgcg
    32281 tggggtcatc gggagcgcac ctaccacgcg gacgaccact gcatcgggca cgcctggctg
    32341 gaactggcgg cattcgacaa taacccggtc tatgccgcgc ggctgcgccg gaatttcgaa
    32401 tacatcctcg cgaaccgcca ccggggttcc cttaactggt cggagaagga ttgcgctaaa
    32461 cgctggtgct ggagcgacgc gcttttcatg gcgccccccg tctgggtcaa gatgtacgga
    32521 ctcaccggtg accgccgtta cctcgacttt ttggatcagg agtacaaact gacgaccgat
    32581 ttgctctatg acaaagaggc gtcgctcttt taccgcgatt cacgctatct gaccgagcgg
    32641 acgaaaaacg ggaagaaagt tttctggggt cgcggcaatg cctgggtcta tggggggctg
    32701 gcactcatgc tccgtgacct gcctgccgat tggcctgccc gggcagacta cacaaagctc
    32761 ttccgggaaa tgtccgcagc cctgaagaaa acgcagggga cggatggcgc gtggcggccc
    32821 agtctgttgg acccggatga ccccgacctg cccgagatga gctgcaccac gtttttcgtg
    32881 tatgggatgc tctggggtgt caacaacggc gtgctcgata aggagacgta cctgcccggc
    32941 atcaagaagg cctgggccgt gatcagtgcc agtatcaacg gggagggtcg gctgggctgg
    33001 actcagccgc cgggttgggc gccggaaaag gccttcaacg cggacacgca tgcggtttat
    33061 gcgatcgggt cttatctgct cgcggccatg gagttgcgca agcagattgt ggcagatgcg
    33121 caccccgaac ggaaaaccgt gacggcgacc aatccgtcgg agcgcttccg gagtgccgaa
    33181 accctctccc tcaactgggg ggagctgaaa ctcgacgcgg ccaagacgcg cgtcttcgat
    33241 gtccgtaatg ccgcctttct gccgcatcag ctcatcgaca gcgatctcga cggcgtgcct
    33301 gacgagctga tcttccggag ttatttcgcg gcgcgccaga cccgggagtt ttgggttttc
    33361 gaggacagcg gcattccgcc gcctgtttcc ccggagatct gcttcagtcg gcatgtcccg
    33421 gagcggatgg atgatttcgc atgggagaac gaccggatgg cctaccggat ctacggaccc
    33481 gccgtgtcgc tgccgccgcc gcagggcgag gggttgcatt ccagcgggat cgatgtctgg
    33541 tgcaaaaaag tccggcaccc ggtgatcaac acttggtaca aacacaggcg gtatcatgac
    33601 gatcacggcg aaggcatgga tttctacaag gtcggtccgg gccgcgggtg cggcggattg
    33661 ggtgtgttcc acgcgggcgc gttccatgtc agcggaaact gggccacatg gaagcacatc
    33721 gcgaacggtc cggtgcggac cgtgttcgaa gtgacctacg cgccctgggc gtgcggaaac
    33781 ggggcgcagg ttgcggagac gcggcgagtg agtctggacg cgggttcgca cttcacccgg
    33841 ttcgagagtc gccttaccct caccggagcg acgggtctgg cggcgggacc cggaatggac
    33901 atctcggtga aaaacgggca taacgggctt ctgacgtgcc gtccggatca ggggtggttg
    33961 gcgctttacg ggcccgagca gaagggggcc ggggccatcg ccaccgcgat ccttctgcct
    34021 gcggcgggga acctgatggg cgacggcggt aagaatgtct atctggttga cgcggtgtcc
    34081 cctgataagc ctctcgtctg gtacgcggga gcggcttgga gcggcgcggg ggatttcgtc
    34141 aggccggagg attggacgga acaggtgtcc gctttcgccg cggcgctcgc ggaaccgctt
    34201 gtcgtgacgg tccggtaaac gcgcacatgt tctgtaaaga agaggaagga tgaggcggtt
    34261 gccgacaggg accgggtgcg gctcggacgg gtgccggaga gtaaaaggag gagaagcgga
    34321 tgagaacgag gagatgggtt gcggtagggt tgatgctggc ctgcgcgtgt cgcgcggaat
    34381 cgctgctgag gccgaatgac cggattattt tcgtaggcga cagcatcacg gcgcaggcag
    34441 tcggggaaat gcagggctat taccatcagt tcaccaacgc gctgaagacg gcttatcccg
    34501 cttcccgtaa tgaggtgatc gggctgggat tctccgggca tacggtcttc agttgggccg
    34561 atagtctgga ggtcaagagc cggaccgaat ccgtaccagc caacaccggc ggcttcgatg
    34621 tccacaccgc gttcgcccgt ccggccgatc tggtgctggt cctgctcggt atgaacgaca
    34681 tcctgcggcc cacggtgaca gatgaggaag cgtcgctgcg gatgtggcag gacaagtatc
    34741 gcgaactgat ccgggcgatc cgcaagcgtg ttagcccgcg cgagtttgtg ctgtgcgaga
    34801 tcacgccact caccgaggat ccgctcgccc ccaagaaccg tgtgcgtgac cggatgacgc
    34861 aacttgtgcg tgagttggcg cgcgaagaga actgccgcgt ggcagaaacg ggcggcgcgc
    34921 tcttcgatgt catcgcgcgt gcccgaaagg cgcgacagga ctatctggtg attccggata
    34981 cggtccatcc gcggcgtccg ctcggacaca ccgccatcgc gcgcggtatg gctgccgcgc
    35041 tacgggatgc aaaactgacc gccctgttcg acgcgcgcct cgatgccgcg atcaccggac
    35101 tccggcccgc tcaggcgacc gtgacctggc gtttcaaacc ccagcccggc atcgaagcca
    35161 agggcgggga gcaagtctac cggatcgagt gctattggga tgacggcacc ggggcgtccg
    35221 ttgcgcgcgt gccgaccttt tcgctggagg tgcccgaggg gtggctcgtg gccgaggcgc
    35281 cggtgacccg gaccgaggcg gtgttcaccg tgcgtggcac gcccgaacgg ttgcagacgc
    35341 cgctgacgat tttggctaag gccggtgaag aggtgatccg ccggacggtg cagatcccgg
    35401 taccatggcg cgtggtctgc gggccggaga atggcggcgc gtggatgagc gggcggcgtt
    35461 atcagccgga taaccgtgtg ccgttcatgg agacggatct ggtggcgggc aagcattttc
    35521 tggaaccctt cacagacggc ggaaaaagct atggctggca gatcaacact ccgtgcatgg
    35581 actacacggc gggcgatgat cccaatgcgg tcgtgcccta cagcctgacc ttcggcaggg
    35641 agaatgacgt gctctacgct gtgcgttggg tgtacagcgc caaggcgcgc ccggtgcaaa
    35701 tccgactgtc acaccgcacc ttctcggcca cgctggggtt cgtggtctgg atcaacggtg
    35761 agcgcgtgat gacagcggat ctcaaccgca gtggcaagaa tcaggcggtc ggccaagcca
    35821 cgctcaaagc gggctggaac cgtctgctgg tgcgcaacga ccacctgcag tggcagcgcc
    35881 agttcgcctg cgccctgctg ccggtcgagg gcgacgcgct tgacgatctg cgttacgcga
    35941 tcacgcctca gagcgtatcc ccgagcccct gaccgttgcg ggttcggggg gcgggctttc
    36001 agtcgggaac acgggcgtcc ccgcccgtaa tggcggatca gtcgccctgc caaacaaccc
    36061 aaatgccgca agtcgacggg tacgagtacg aggacgggaa aacgatcccg aatccgattt
    36121 cgatagcgat aaacccggaa cccgaaacca ccttcccgcc ttcgctcggg aaaggtccgc
    36181 ggcgttttct tccaagtccg ttcgggactt gtctaatccg ttgtggaccg gtatgatatt
    36241 cattttccct ttcggcaaat gatgaaagtt cggcacgata tgagaatttt atctggaatc
    36301 caaccttcgg gcaaactcca cctcggcaac tatttcggga tgatgcggcc tgccctgcgg
    36361 ctgcaagagc agggcgaagc gtatctgttc atcgcgaatt accatgcgct gacctcggtt
    36421 catgacccgg aacgtctgcg gcaggacacg ctggatgtgg cactcgattt tctggcgtgc
    36481 ggtctggatc cggagcgaac ggtctttttc cgccagagcg acgtgccgga ggtgcatgat
    36541 ctggcgtggc tgctctcgat cgtcacgccc atgggactgc tggagcgttg ccactcctac
    36601 aaggacaaac tcaccaaggg gattgcgcca acgcatgggc ttttcgccta tccggtcctg
    36661 atggccgccg atatcctgct ctaccaatcc gacgtcgtgc cggtcggacg ggatcagaag
    36721 caacatgtgg aggtgacgcg tgatatcgcg atcaagttta acaacgagtt cggcgaggtc
    36781 ttccgcatcc ctgagcccgc gatctccgaa gaggtggccg tggtgccggg tctggacggc
    36841 cagaagatgt ccaagagtta cggtaacacc atcgagctgt tcggcgaccc gaaggaggcg
    36901 cgcgcgcgca tcatgcggat cgtgacggac agcaaggggc tggaggagcc caaagatcca
    36961 gccgcctgta acatctttgc cctctaccgg ctcttcgccg atccggcgcg acgcgcggag
    37021 atggaggcga actaccgcgg cgggaattac ggatacggac acgccaaaaa ggcgctggcc
    37081 gcgatcttcg aggaaacctt cgcgccgttc cgcgcacggc gtgaggaatt gcagcgcaac
    37141 ctcgattatg tggaggaggt cttgcgcaag ggggccgcgc gggcgcgtgc cgaagccgcg
    37201 aaaaccatgg accgggcgcg ccgggcggtg ggcatcgcgt gaccgatggc gcggggcccg
    37261 cgccgcggag aggggtagac atgtcgatcg aggcgctgtt gccggatgag tacaaggtgg
    37321 atctggaggt ctttgagggg cctctggact tgctgttgta tctaatccgg cgcgaggaga
    37381 tcgatatcta tgacattccg atcgaacgga tcacggcgca gtatctgacg taccttgatg
    37441 tgatgcgcat gctcgacctg aacatcgcgg gcgaattcct ggtgatggcc gctacgctga
    37501 tgatgatcaa gagccggatg ttgctgcccg tggaaacgcg cgccgtcgag gaagaggaga
    37561 gcgacgagac ttgggtggat ccgcggctgg atttggtgcg gcaactgatc gagtacaaga
    37621 agttcaagga tgcggcgaac cggctgttgg aatgggagac cctgcagggc gaggttttcg
    37681 cgttcggcgg cgcgcagccg attctcgagc gcgatccgcc ggacgcgggt caggctttgg
    37741 gtgacatcgg tctgttcgat ctgctgaccg cctttcagga ggttctggcg cgtgcgccgc
    37801 tggagccgct gggccatatc gagccgatcc gctggtcggt gccggacaag atggagacca
    37861 tcctgaccct cacgcgcggc gggaatcgcg tggcgttctc cgcgctgttc aaccccgaat
    37921 cgcatcgggg tgaggtgatc gtgacgtttt tagctctgtt ggagctgttg cggctccggc
    37981 agatcaggtt gtggcagaac gcggcgttcc acgaaatcct gatccttccg gcggaggaga
    38041 tggatggcgg accgttaccc gttcccgtga tcgaaggaga cgaacatgtc caagcctgat
    38101 gaggtgaaag tcccgttgcc cgcaagcctg cagcaggtgg tcggggcgtt actgttcggc
    38161 gccgaccatc cgctgacggt ggaggagatc cgtacttgcc tgaagaatgt ggcggaagac
    38221 gcgtccggag aagagaaggc ggccctgtcg atctttgccg aagcctcgcc gcgtgagatt
    38281 gaggatgcgg ttgggggaat caccaaggag ttgtctcgcc tcggtcttgg catcgaagtc
    38341 acggccgtcg gcaacgcctt ccggttccaa actcaggcgt gttgcggccg ctgggtgcgt
    38401 agcctgctca aggccgaacg ccccagccga ctctcgcggc cggcgttgga gacgctggcg
    38461 atcatcgcct accgccagcc gatcgccaag tccgagatcg aatcggtccg cggcgtgacg
    38521 gtggatcata tcgtcaaagc cctgatggaa ctgcacctga tccgtattgt gggtcgcagc
    38581 gaactgccgg gacgcccctt cctgtacggt acgaccgcct cattcctcag tcatttcggg
    38641 ctcggcagtc tgaaagaact gaacgatctc gaccccacgc tgcaacgttc caaaccctct
    38701 gagcgcaagg cgatccatca gaaaaagaag aagaaggatg agtcaaagga cgcttcgcag
    38761 gacgcggcgt tgccgtccga cccgcccgcc tcggaaaagg gggcgaccga ggcggtcaac
    38821 gagatcctcg gcgatggctc gccgcccgga gagaacgccg aaccgctcta cgaggcgtaa
    38881 acctaaaaag agcagttgaa ccttcaactc tctttggttt caaggtttct ggtttcatgg
    38941 tttcagggtt tcatccaaat cggggtcggg atcgctatcg ggatcgggaa cgtcgacccg
    39001 cgacccgcga cattcgaccc gcgacgatcg acagggacgc ccgcgtttcc gacattgcag
    39061 gtcgctcgtc gcgggtcgac ataaggtctg acgggagaag cgggtgtccc tatcccgcgt
    39121 ggcggggcgg ttgcccgaaa ccgccgccag tccctaccga acggccgcct cggggatgcg
    39181 tccctaccgg ttggcggacg ctttgggcgc accgtaaaac ccaatgttta cggggctttg
    39241 ctggaaatct acccgttcaa ctgccctttt tggcgtaaac gcagggaatc gaatcggtgt
    39301 ggattccgaa gtcgtatgcg aagcttggtt gggtgtcctc gtttccgttc ccgccttctt
    39361 ccggtctgga aaggggtttc tggcaacccg gacagtcgcg ctctccgtct ttccgcggac
    39421 tcgggccccg gctctcttcc gtccacatat catgagaagt ggacagccat tcgcatgacc
    39481 aacgtgatct aaaaaacaaa acctgtcttg atcttttttc tcgttcccaa cgttgcggtg
    39541 caccaacggc gcggtcagca ccgtcgggtg tcgacgcggg ttagcacgtt gtggtcatcc
    39601 gttctgatgc aggcgattcg gcgcagttgc gcaatacggt acaaggctgc aggcaacacc
    39661 cagagacaca tcgttacaca gagaaactgg ctcacctcca cattccccgt cttgcttttg
    39721 gcgacaacga ccgtattcga gattatggct atcgtcacga gtgcaataac cgagatttga
    39781 agtgctcgca aatgacggag cgacaaccga cgccgagctt cggcatccca cagaatccga
    39841 ttcatgtctt ttgttccggt gtccatcttt ctcctatgcc aacgtattct gaccaaaggt
    39901 gtcttgcata acacctctgc cagaatgagt cattatttac aaaaaagggg cgtggcaacg
    39961 gtcagcgggc gaaacggggt ggctgcttac cggtcgcggt agaccacttt cttggagccc
    40021 ttctcgatca tgccaatgac cacgggtgcc tgccgctgcg ccttcaacat tttcagcgcc
    40081 gcggtgagat ccttgcgccg cacgatgatc acaaaaccga tccccatgtt gaagacgcgg
    40141 tacatctcgt cgcgctccac cttgccttgg cgttccagaa aggtgaagac ggtcgggggc
    40201 tgccaactgt tgcgatcaat gaccgcattg caggtcggag gcagcacacg cgggatgttg
    40261 tccacaaacc cgccgccggt gatatgggcc atgccgcgga tcggcacctt ggcctgaagc
    40321 gcggtcacgg gtttcaggaa actgcggtgc agggcgagca gggtgtcggc caccgtcatc
    40381 cgcgttccgg ggatcaggtc ctgcggcgtg agtccgcatt ggtcgatgat gactttacgc
    40441 gcgagcgaga agccgttggt ctgaagaccg ccggagggca aaccgatgat cacgtccccg
    40501 acgcggatcg aagtgccggt gatgagcttc ttgcgctcca ccgtgccaac gatcgtgccg
    40561 accagatcgt actcgccctg aggatagagc ccgggcaact cggccgtctc gccgcccagc
    40621 aacgcgcatc cgttttcccg gcaggctttg cagagccccg cgaccacgtc ccgaaagata
    40681 tccacatcaa aacgtgccat gccgatgtag tccagaaaga agagcggctt cgcgccctgg
    40741 accagaatgt cgttcacgca gtggttgacc agatcctgtc cgaccgtgtc gtgttttttg
    40801 gccagcaccg ccatcttgag cttggtgcca acaccgtctg tgctcgcgac cagcagatga
    40861 tctttgccgg gcgagtggaa aagcccgccg aaattgccga tgccgccgac cacacccttc
    40921 gtggttgttg cccccaccat ttttttgatg atctcgatgc cagacatctt cgagtcgata
    40981 tccactccgg cttgcgcgta cgccgaaaca gcacgattgg gtgttgtccg ttttgtcatc
    41041 cgagatcctc cccgacaaga atgagtgttt gaaaattgct gtaaaatacc atttccttcc
    41101 gatccgttcc agccaaaaag gacaaactat agtttgtcga tagacttata cggcaaaaac
    41161 agcaggttta aggggggatt ttgcaacaat gggatagcgg aattatcgga ttgtgggtgt
    41221 gtggaaagcc ttgcgggcga gacacatacg cacaacccca cacacaatat gtgtgagatg
    41281 gaatcgggtg gcggattctg tcgcgttgcg ggtcacggat cggcgcgcgc gtccgtcgaa
    41341 atgtccagac gcacggcgag gacgttgttt gagaagacga tggcgtcatc gaagaaaaag
    41401 atgcgccatt cggaaccgtc gagtttgcgg aattcgaggt ccagacccaa cgggtcgctg
    41461 cgccggatgg cgggctggcc gagaaaccgg tgctgcggcg cttccgcgag ggccaccacc
    41521 tcacgccgcc ggtcgaccgc gaaccaggcg taacgcccag tccgttgcgc aggcagcatg
    41581 atgtagtaac gagtgggcat ggccagcggc cggtagagga cagcgtcggt cagcacgcgg
    41641 ttatcgaaac ggtagctcag ataggggaag ttggcgcgga tcaggggcgg cttgtaccag
    41701 atggccgcgg caaggacaat gaccgcgaga gcgatcgcgg ttttggtcag gtctggcgcg
    41761 atccggcacc agcgggaacc gatcgggaaa agaggtttca ccacaggcgc gggttccgga
    41821 atcccgtccg accgatccga caggggttcc ggtgccgttg tccgcgaatc cgcatcggag
    41881 aaagaaacgg cttcggcggc atcgccacag gctcgctccg caaccggtac cccggccgcc
    41941 tgtccggtgc ccgtcacgtt ggcgcgtcgt ttcgattttc ccgttttacc cattcgtcgt
    42001 aagttataaa cgttggccgc gccggggaaa aggcaaaagt catccgaaat acaggttcca
    42061 ttcgtacacg gtatacgaat ccgcctgaca gcgggaccat gcgaacgtga cgtcaggcga
    42121 acgtaacttc ctgaccgtga aacaggaagt tgccgcccac gacgtactgc attgcggtcg
    42181 gggagaaacg gtcctgcacg gtaatgtgga gatgaccggc gagaatggct ttcagtaacg
    42241 gttcggtctt gagatacgca atgaagtcac gcgttgtcgg gtcggtctgc tgcgtcttga
    42301 agtcgccgct ggcatcggga atgacggcgg actcgaactt ctttccggaa cgggcccaga
    42361 acttgcggct cgcgcgccag aggccgtctg tgtagaacgg cacatgcatg cagaggatga
    42421 tcggcagccc ttttttcacc tcggcccgga aacgttccac ctgatcgggg cagacataac
    42481 cgtagacgga gtcgagcgtc acgaagttga cgccgttcac cacctgcgca tgcagacgca
    42541 gatcgaaggg aaacgcctta ctgagcagtt ccgcgctgcg tgctttgtac gcctccgtgt
    42601 tttcgctcgg ctccagccac atgtagcgcg agaactcgtg gttgcccaga gcccccagca
    42661 ttccggcacc gaaggactgc tttacgagat cgagattcgc ctcgctcagc cagtcgatca
    42721 ggtcgcccgt gtgaaggacg aagtccacgt tgtctttggc ccatgcgagc gagtcgcgca
    42781 acgcctcctc ctgccgtccg ccgaacgtct gcgtgcgggc tgccttcagc ttttgtttcc
    42841 gctcatcctc atgcgggtat gccgccgtga agtgcgtgtc ggaaaggtgg agaatcgaga
    42901 acggccgggt cgcgcccgcg tcgatgtgaa cgtaggcgag cgaaagacgc tcgaaccggc
    42961 cgcgcttcgg aaacagcgcg ggatcatccg cgagcgacgg cagacgggag gtggcgacca
    43021 gcgcgcctgc agacaaggcg gttcggagga agtcacggcg ttgcataggc ctgttcatgg
    43081 gtgtctcttt ttcatgcttt gccgggcggc aagttagatc atttcggcat ctgtgcgcct
    43141 ttgccttcca ccgcgccgaa gagcttcagg gcatcggtga ggggatagcg gttggcgaac
    43201 atatccacac gggtcgttcc cgtggcctgc aggttcatcc cgaacgcgct gcgaacctga
    43261 ttgacgggcg aattgcgatc caacagaacc gattcggagt tgttcgcgct gacaaagccg
    43321 ttcagatagg cctcgttgat cttgcccttg agagcttccg caggaagttc cgcgttcccc
    43381 tcgtacttat agagttcttc gcggtcctcg aagtccttgg cccggattaa cagccacttg
    43441 gcgccgcccg gaagcacgag gtcaccctgt gtgttcagga agaaggcgtt gttttcagca
    43501 ccggtcttct tcatcttctc atggtctttt gagttctcaa tgcgcgcccg gtccaagccg
    43561 ccgagcgtgg aagtgccgat gatgcagtgg tggacatcgg agtggacgtt gttcatgtag
    43621 cggaacccgt accccatgtc acccagatca ttggtccgcg cccagttaaa gagcatcgtg
    43681 ttgtaggcgc agtcgatggt caggctgaac tcgccgctct tgccgccgcc ggacagctcg
    43741 atggccgcat aggtgttgtt gatgaacacg ttgttctcga tgacgacctt gccttggctc
    43801 catgagccgc ggatggcgta attaccgctg ttggcgaacg tgcagttgcg gatcgtcacg
    43861 ttgccgaatc cgttgccgaa gtagatgagc ggctggacga tcgtcgccac gttcttcgca
    43921 tcgccgttga tgccctggcc cggaggattg acaagcattc ccgtttcgac atccttgggc
    43981 ttgcccttcg tcggatggta gccgttcgag aacccgcggt cgaagacaat gccgtcaatg
    44041 gtgacgccgc cgtcaccggc gttgaaggaa cggggtttgt tggggtcgcc gatggacagc
    44101 agggcgaacg agcgcgaggt gccgttctgc gcggtggtgg gctgcaccat ggtgaggttc
    44161 ttgagcacgt cgcggcttgc gaagtcgtcc gtataaccgc ccatgatgtt gaccgggatg
    44221 gtcattttca gaaatccctg gtcactggtg ccgaggtaat tgcccgcagc gacaaggatc
    44281 gtgtcgcccg cctgcgcctt gtccagcgcg gcctgcaagt cgcgccacgg cttttcctta
    44341 ctgccgtccg ctttacgcac actgccgccg gttgcggcag agacgtgcag ggttgcggcg
    44401 tgaacgaccg ttgacgcaga caggatgaac gcggcggcag aaacaaaaag acgagaggtc
    44461 ttcatcatgt gatactcctt tttttggtgt ggaaaactcc taactcctaa ctcctaactc
    44521 ctaactccta actcctaaat cctaaatcct aacttccttg gccttggcct tggtcttgag
    44581 ccagacaacg cagccgacag acgcgaccag cagcgggatg ccgatcagcg gacggtaggc
    44641 gagccaggcg atcgagatcc acagaaacga ccaggccaat ccgacgaggg tgcagaccaa
    44701 gccgacaccc gcaccgacga tgtcgcccag aatcgggatg accttggcga tcgcctccaa
    44761 gatgccgaag atcgacttca ggccgatgca cacacacagc acgccgacga gccgcaggat
    44821 ccacgtcagg atggcgttct ccttggctgc gctggcgaac atctcctccg cactccggac
    44881 cccgtcatcc agacgcgaga cggtcttgcc gtttttcgcc acgaatttca cgaacgtctt
    44941 gccggagacc ttgccgataa tggagacctc atgcggagcc acccaagtga acgacacgcg
    45001 gacatcaccg attaccggcg caccgggcga acgtccgaag tacacggtgt tcccgctgac
    45061 gtgaattggc ggcgtattcg tcgagcttag ggcgcgtggg aagcgcgggt gcgcggacat
    45121 gctctttgcc catgcctgaa tctgcccggg cgcggggttg acattcgcgt gctctgcctg
    45181 accgatggcc tccacaaaga attgaggtag cgtgtaagcg ccgaatgaga cgttcttcgc
    45241 gtatgacgtc tgcgcgtcaa acgtcatcca gacggtgtta cgattctggt agtcgggatc
    45301 acggaactcg cctgaatcca ctggcttgga tcgccactcc tttttgtacg tgtaggtggt
    45361 cacccgctcc tcgccgccgc ccatcctgtc gcgggtttcg gtacgggact cttcgaccca
    45421 ctggtagtac tccacgctgc ggcggagttg gatcgccgtc tgtttcacgc cgaattcagc
    45481 gtcttccaaa acgtcctgcg tttccgcacg ggcgctggcg tggatcagct tgccttcgag
    45541 agcgggatca agagaggtga cctgctcaac ctgcacaacg gcatcctgcg cctcgttgag
    45601 cgcttttttg gtcttgacga agtttccttc attccagaac agcagcgccg tgcctccgat
    45661 aaacatgaca gccccgccga ccacgccacc cagcgctctc ttcagacggt ctccgtatcc
    45721 tgtccttgtc acttcttgat aagccattat tgctcctcgt tatgcaactc caaaacacaa
    45781 gccagtgatc gtcattgtgg catatcaccc aaagcctgtc acgcgacatt ttatctccgc
    45841 gcacagcgcg gagttacgaa tccggccaca aacagcattg cacgttgaac accaagatgc
    45901 ccctgctttt catgaaggtt ccgttttaga gtttcggggt ttcaggtttc agggtttcgg
    45961 ggtttcaagg tttcatggtt tcatccaaat cggggtcggg atcgctatcg ggatcgattg
    46021 cttaacctaa aaagagcagt tgaa
//
LOCUS       DAOSLE010000006        42597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1052_length_42597_cov_7.432819, whole genome shotgun sequence.
ACCESSION   DAOSLE010000006 DAOSLE010000000
VERSION     DAOSLE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 42597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42597
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_42597_cov_7.432819"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(627..1715)
                     /locus_tag="PK576_00870"
     CDS             complement(627..1715)
                     /locus_tag="PK576_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60174.1"
                     /translation="MGDRGFDWRRRIGWGIVVAGVACVCGVGCLTEKTHAPETTAGHE
                     LKGMSEEDRIARLRQAGFVCERVSGDMADGWRVDLGGAYKKRVATRDLRPLAGLRVVE
                     LGAQYSEVEDLSPLRAMPLKKLDIRSSRVSDLSALKGLAITNLNLHRCCPVADLSPLQ
                     GMPLVDLEIGQTSVRSLEPLRGMPLNRLHMEVTDIPDLSPLKGMPLARLFINYTTVTN
                     LSDVVGLSLKELHMGWTRITDLSPLVGMPLERLHIPGTKVCDLRPLKGSALRFLYMSG
                     TPVTDVEPLRGLPLYFINMTDSAVSDLTPLADLQLRQFLFTPRRIKKGIEAVRAMPTL
                     RIIGVGFSRGTMWSPSEFWAKYDAGEFE"
     gene            complement(1734..2714)
                     /gene="hemB"
                     /locus_tag="PK576_00875"
     CDS             complement(1734..2714)
                     /gene="hemB"
                     /locus_tag="PK576_00875"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOE60175.1"
                     /translation="MRFPEVRLRRLRRTPAIRRLFDDPVPPASSLIWPVFVVEGHNRC
                     EPIDAMPGQNRMSVDHLLRALEPVAAQGVGGVLLFGQTEQDKDEGGSGAFDERGVVQQ
                     AVPAIRRAYPELVVMTDVCLCAYTAHGHCGPLAPDGSVDNDAANGLLARVAVSHAAAG
                     ADVVAPSAMMDGQVAVIRAALEENGFDQTLLMAYSTKFASSLYGPFREAERSSPSSGD
                     RQGYQASGGNLRAALRESDFDEDEGADILMVKPALFYLDVLSRLRERTDLPLAAYNVS
                     GEYSMLIAAAERGWGDRDAMARESLTAIRRAGAEIILTYWAPRYRELFKL"
     gene            complement(2728..3342)
                     /locus_tag="PK576_00880"
     CDS             complement(2728..3342)
                     /locus_tag="PK576_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOE60176.1"
                     /translation="MRRRERVLLLLAAGLGCAAGCQAPMFEGTMFEAQPRQDPTVARI
                     ATSTQQMNLEFRQLAEQVAAVSRNQEVLDARLTRLEGQLAALQRSGEEMGALRQELQR
                     VRVEREGLRKEITDDLAGRIETIASRQQAEIRAARAAAERAAPSASASPARSGSGYEH
                     KVERGQTLSQIARGYGKSMESIMKANKITNPSAIRAGQILFIPD"
     gene            complement(3363..3692)
                     /locus_tag="PK576_00885"
     CDS             complement(3363..3692)
                     /locus_tag="PK576_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60177.1"
                     /translation="MPPATGRRVLKQGPNSVFVDADVRRTPASWRLDVREDAAGNSFF
                     FGKRRRPALRSRGRNRLFHYIAAGGLRQLRRTSADDDREARRRAFLVFLALLSAVWLL
                     FYFLPCL"
     gene            complement(3712..4872)
                     /locus_tag="PK576_00890"
     CDS             complement(3712..4872)
                     /locus_tag="PK576_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOE60178.1"
                     /translation="MVRYGIIVWGSLLLLSGAGCGKSGVQEAESAERGSPLYRKAFAA
                     EQSGDLKEAIRLYNRVLIEEPRSFSAHFQLATLLHDHAEDYVGAIYHYNQYLYLRPEA
                     EKSTLAQDRIRIAEQLLAPQILRKVGDSVQGISQAHLLKENDRLNRLITTLEGEKSML
                     VESKTAAEQERTRLQAENERLRDLLRKMRVEEVAAPEAAAGQAAQAARDAADTRESKR
                     TTDARTLRALREEAAELAAQSEKPQPIRKPVVDAPTTESVLRKVQSRLTGGSDDSVPA
                     ARKEQSRLASGSGDSAPAAQTVAPPPPIDVTPDKDALSALSLFGQDGRKEKRTRKAGA
                     HRTYVVQPGDTLFRVAERFYGDSTQWKRIRDANRGRIDADGRIRAGQIIEVP"
     gene            5027..6841
                     /locus_tag="PK576_00895"
     CDS             5027..6841
                     /locus_tag="PK576_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE60179.1"
                     /translation="MTTENTKKLWDSARAVLGGNGCLSVIMPVYGLEKIIGQNIQAVC
                     DLLRDEIPFEVLPVDDGSRDGTAESIRQAASRDPEHVRPVFVQVNAGKGNALKCGFAS
                     SRGSHILLLDGDLDLSPARITTFFEVMAHNRADIVIGSKRHPESVIDYPWHRRLASVI
                     YYTLVRLLVGLPVTDTQTGMKLFRREALGWAFDRMLVKAFAFDLEILSIAHARGFKVA
                     EAPIEMHFGNKVGCLSWSNVRQVMNDTLAIFYRLRILRYYGSVEVAPPASRPMTVSVV
                     IACPGPSPYLTECLEALALQTHRAFEVLVLPDEPLTLPEYPFTLHVLPTGKTRPAEKR
                     NTGLWRAQGEIVAFLDDDAYPVPAWLEHAVKYFTLPDVGGVGGPGVTPPHDPFLAQAS
                     GRVYANRLVSGNYRYRYLGDRVRPRIDDFPSCNLFVRTEVLKAIGGYRTDFWPGEDTL
                     LCADVVFGQGKRIVYDPWVQVFHHRRPLFGPHLRQIGRYALHRGHFAKRFPNTSLHLS
                     YLLPSLFVAGVIAGAILSVFYPPLRIPYIAVLATYAALTLLSSLTLHPGLWFVTWLGV
                     MGTHLVYGVRFAQGLLTRRMPCEAGAFDHPSEKPSGAT"
     gene            6838..8070
                     /locus_tag="PK576_00900"
     CDS             6838..8070
                     /locus_tag="PK576_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HOE60180.1"
                     /translation="MILPRKKRRNTRQRIPQVALLLESSHEISRGMLRGILEYVHLYG
                     PWALHLNSGGANDQKLPDRAAWRGSGIIARIPNPHVAADIVAARLPTVLIDPVDAYLN
                     PRHPLARCCRVSCDSAAVARCAADYYLANGFTRFAFVGEPSGLNWSLWRQKAFVNRIA
                     EAGFACDVYPPPSEEERATWELDRKRMCRWLAKLPKPLAVFAANDARGRQVLNACLAA
                     DLPVPYEVAVLGVNNDVMICETSLPPLSSVAVDMERAGFAAAELLDQLMRKTLRKQRC
                     VTFAPQKVVARASTRRLQVNDRLVIRALEFIRINAGLNVRVSDVADHVGVTQRWVEKR
                     FAQTLNRSVMDEIQRVRMETVRSLVTKSTLTFSAIAQRCGFTNANHLGVLFRHTFGTT
                     MSDYRHQHAPPTLTARPR"
     assembly_gap    8111..8210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8314..10215)
                     /locus_tag="PK576_00905"
     CDS             complement(8314..10215)
                     /locus_tag="PK576_00905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE60181.1"
                     /translation="MIIRMCGLVALAVCGAEDPVCVTRQGLPNASRIWRAKTQNGCVF
                     YAGCTTQTPRGQVRNRVPPLVVRAFPGAKGVFPFISWGRAYPDNCSSVIETFRAMSDG
                     PSVSPETSWPNVALTLIDLSDQDIGQTERQVRAALEGLVHRTRQRNPARDMLMLHQPE
                     ASFVAAYRAGRTPDVIRWYEEIAEHYGIPSVNLAKAAARKPEWKGAQEEWDALLRDEL
                     GALFSWCAAQPAQAQPVRHACPSPLTPALWNRATLVNYERGDLDPAGWLGWQWSPVDA
                     IFHVAVCEKPGPVMTLYFTGTAAGLYGVTGPDSGDLEVAIDDGPWQRLAVFDPQARDN
                     AYHLFHRMFADELPDTRHAVRIRVAEAVPSGSTGRVARIGWLTVNGRDASPMGSLSPV
                     ALADTLFLQLEPIAYRPPPGRWSRIPKTMERLRSGGTVRMVMLGDSIINNIQSSHFER
                     LIERAYPGSRIEKIVSVRGSTGCWYYQEPEHLQNYVLRHKPDLLVIGGISQKGDVEAI
                     RSVIRQTRAALPEVEVVVMTDVYGGPWAKDLDPYDPKNAADPDPAGTGYRDRLFKMAG
                     EERVEYVNITQPWMRHLTASKQPLGAFKSDAVHANARGSQLIGRFLELYFAPDGWDAY
                     GTAKRALKP"
     gene            10454..11959
                     /locus_tag="PK576_00910"
     CDS             10454..11959
                     /locus_tag="PK576_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE60182.1"
                     /translation="MNEPSLTLLAIADLHFTGLARQTAQPPQLRGELAQVLLRKVFAR
                     LTHMGVKPDLTVLLGDLTEDGTDPQAKLDLISLNGELTRSGIPVLALPGNHDIAPAAF
                     HRLLGCEPGLHLFGGYGLVLFDDVYGEDRTCARTEDALAMLRQIAADHPDLPLIALQH
                     PPIYPAIDSHYPYRPTNAAEILDTFEKSKVVLSLSGHYHKGQKARMHEGVLYHTVPSL
                     SEAPFRFSLIRLTGHRVEIEEIQLAMGLPNLIDVHCHTEYAYCGTTVNTASCIALSQA
                     LGVTGLCITEHAFQLYFDKKEAMGFAWQRNPEMAEAVWATPERKRMAAYKRFAQTLRS
                     PFVKVGLEVDLYDHGKLLLAPEDAESEWDLLVGAIHLIEDYVPGRTSQKEAERLFLRD
                     VELLTGHPIQVLAHPFRFFSRNRLACPTHLYAAVADLLADTGVAAEVNFHTHQPDPDF
                     LRICVEKGVRLALASDTHDLAEAGEFWPHVQVLKQAGVTPRSFPETLFRFT"
     gene            complement(11973..13775)
                     /gene="ptsP"
                     /locus_tag="PK576_00915"
     CDS             complement(11973..13775)
                     /gene="ptsP"
                     /locus_tag="PK576_00915"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE60183.1"
                     /translation="MSNDSDHLTPSRNEARVLTGLAASRGFVAGPAFLFRTTTTEQVP
                     EYGIRPEQVPHELARLADAFTLTRAQIRSLALELSKRIRGDEATLFDGHLMILDDPSF
                     IGSCKEKITTKQVNAESAVNSVAERYASIFAAMDDAYLKERSQDIGDIAKRILRNLLG
                     GADPLAVRVEKPCIVVADELSPSETISLPRNLILGFATDRGSNTSHVSLLARALGIPA
                     VVGLGSISEQVRTGDALLLDGTSGKVILNPGQQERLAFEKMVARSRTLDEKIEEGKCL
                     PGATSDAHRIPMLANIDPGTPLTDLYAVGAEGVGLYRSEYLWLTYDQEPTEEEQTRAY
                     TNVARVFPDGQPVTIRVLDLGGDKMPHYTGALYKEANPFLGNRSIRYLLSHPEVFRRQ
                     LRAILRASAHGNVQLMYPMVATIEEVRAANLEVRACMSALRAEGIPFNETIRRGVMIE
                     TPAAALIADALAAECEFLSIGTNDLIQYTLAVDRVNETVARLYQPTHPAVLRLIDLTV
                     CAGRARGIPVSVCGETAADPVMAVLLIGLGVDALSMSPNLIPLVKKVISQVSIEEARK
                     MAAIVRRMTGTSANKIYTYCRNHVLKLAPDLLYL"
     gene            complement(13765..14061)
                     /locus_tag="PK576_00920"
     CDS             complement(13765..14061)
                     /locus_tag="PK576_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE60184.1"
                     /translation="MGKKDTDAETREFVLLNKYGMHVRPAGLFAKVASRFNAEVEVEK
                     DGNVVSGKSIMALMTLEAVCGTVLRVTAHGPQRREALDELESLVARKFDIPDEQ"
     gene            complement(14065..15036)
                     /gene="hprK"
                     /locus_tag="PK576_00925"
     CDS             complement(14065..15036)
                     /gene="hprK"
                     /locus_tag="PK576_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOE60185.1"
                     /translation="MAKSRQSKVRQSKVTVGQFLTSGRDRLNLELVSGARGLERVIVE
                     PVTHRPGLALSGFFGHFARKHIQVVGMAEHAYLSSLPEALRVQRFEKLFAHKIPCMVF
                     TRGKRVFPEVLRVSERDGVAVLRSGMVTRFFIHAATFVLEGLSAPRCKVHGTMLEVAG
                     VGVLIEGAAGLGKSETALGLVKRGHALVADDLTMLRRDSNNHLIGSAMEVTQYYMEIR
                     GIGIIHVPSIFGVAAVRGEKQLDLVVTLKRQGEADAELDRTGTESMRRTFLGITVPQL
                     VIPVAPGRDLVNLVETAAQEHKLRLSGFVAVRELDARIKRHHAGGEG"
     gene            complement(15036..15350)
                     /locus_tag="PK576_00930"
     CDS             complement(15036..15350)
                     /locus_tag="PK576_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOE60186.1"
                     /translation="MVIEVTVRNKQASPDVKKYAEKRALKLFETFPRVESVRVVIDVQ
                     RNLYEAEFVVQQKGLTAVGVKGHASSARAVIDVAAARVEKQLRKTLSKRIKANVRKAA
                     KV"
     gene            complement(15402..16157)
                     /gene="lptB"
                     /locus_tag="PK576_00935"
     CDS             complement(15402..16157)
                     /gene="lptB"
                     /locus_tag="PK576_00935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOE60187.1"
                     /translation="MEDVVENGGTRIPDIQAIELVKQYGSRTVVNGVSLHASCGEIVG
                     LLGPNGAGKTTTFYMVVGLVRPNAGEIVFRGEPITRMPVYRRARLGLGYLAQEASIFR
                     KLTVEENVLAILETLPLSRAERAARAEELLASLSLTKVARQRAYTLSGGERRKLEIAR
                     ALVRKPAILMLDEPFSGVDPLAVHDIQEIVRGLRDKGLGIIVTDHNVRETLSIVDRAY
                     LVYDGQILCEGPSSFLVQDETARRLYLGKHFRM"
     gene            complement(16174..16710)
                     /locus_tag="PK576_00940"
     CDS             complement(16174..16710)
                     /locus_tag="PK576_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOE60188.1"
                     /translation="MFGIVGWVVVLLICRAAWGEVRTNRAARAASLLAGDSTGAPERE
                     AVITAERIEFDNKEGVILFDEKVLVDDEQFIMRSDRLLVFMEGTNDVQQIMAVGRVSI
                     TNANRSASCDKAVYTKKDGQIVMTGNARLMRQGDEGGEVTGNRITFWLDDERMEVSPG
                     RVVLPPGTFKRMDTKRLP"
     gene            complement(16716..17267)
                     /locus_tag="PK576_00945"
     CDS             complement(16716..17267)
                     /locus_tag="PK576_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60189.1"
                     /translation="MVWYVNPLRRAARRLLFLWACLLLGRVEAARFVDPETDRWFARH
                     GDDLWRPLAARWDEVKAAMTSPAKKLSIPLEYHVNGRAKALLRADQAQIFPDGMIFAE
                     KVRVEMFSETGKPDGLLTAEGCLFDRTARHGYCRGRVRVEKGGDRLQGVGMYFSTERQ
                     CIKILGDCEIRTHRIRNNFGRLK"
     gene            complement(17251..18072)
                     /gene="kdsA"
                     /locus_tag="PK576_00950"
     CDS             complement(17251..18072)
                     /gene="kdsA"
                     /locus_tag="PK576_00950"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOE60190.1"
                     /translation="MGRVSRVKAGCVTFGAPELAIIAGPCVIESAEATIGLAAKLVRL
                     ANELEMPLVFKASFDKANRTSGDAYRGPGLIEGLAILREVRDRFRVPVLTDIHEPGQA
                     AAVAEVADILQIPAFLCRQTDLVIAAAETGRVVNVKKGQFLAPEDMVNVIDKITGTGN
                     RRILLTERGASFGYHNLVADMRSLLIMREFGYPVIFDATHSVQRPGGAGKMSGGDGRW
                     APALARAAVATGVDGVFMETHVNPAEALSDKANAIAFRDLKAVWRTLKGIHGLVR"
     gene            complement(18084..18833)
                     /gene="kdsB"
                     /locus_tag="PK576_00955"
     CDS             complement(18084..18833)
                     /gene="kdsB"
                     /locus_tag="PK576_00955"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOE60191.1"
                     /translation="MRVIGVIPSRWGSTRFPGKSLHPLCGKPLIQWVVEAATRATLLD
                     EVLVATDDERIARATEGFAAVVMTRPDHPSGTDRVAEAARAAKDDIVVNIQGDEPLID
                     PDLIDALVRRLRDDPGWAMATAASPIRSLTELESRNVVKVVVAHDGGALYFSRLPIPC
                     RRDGEPDLASGLYLHHVGLYAYRGAFLRRLVTEPPCALEQCESLEQLRALYLGGRIAV
                     ILTGEQGLGIDTPADAVAVEAILKERGQAQQ"
     gene            19053..20129
                     /gene="serC"
                     /locus_tag="PK576_00960"
     CDS             19053..20129
                     /gene="serC"
                     /locus_tag="PK576_00960"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOE60192.1"
                     /translation="MARVYNFSAGPAIMPLEVLQEAQAELVDYKGSGMSIMEMSHRGK
                     EYDAVHSEAAANIRELMGLNDDYAVLFMQGGASFQFAMLPMNFLGEGRTADYVNAGSW
                     GAAAVKQARLIGNVNLAADCQKDIPTRMPDESEMKWTPGAAYSHITTNETISGAQFKT
                     IPETGSPLVADMSSDILSRPLDYSRFAMIYAGAQKNLGPSGVTLVVIRKDFAEQGSIA
                     LPTLLRYQTHIENLSLYNTPPCFSIYMLCLVTRWIKKQGLENLCKVNRDKAQQLYDVI
                     DASGFYRGTAKREYRSDMNVTFRLPTEELETLFIKQAAEAGMKQLKGHRSVGGIRASI
                     YNAFPVAGIEALAAFMKDFEKRNG"
     gene            20201..21973
                     /locus_tag="PK576_00965"
     CDS             20201..21973
                     /locus_tag="PK576_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE60193.1"
                     /translation="MRFPYPMLMLLSAALVSRSDDAYPKPGWVEKPDPLASPYAVPGG
                     MLRFAAFQPPKSLNAYIDSNTYTRQVFGLMYETLLATDPVTTEFTPWLACRWTLSDDK
                     RCYTFELDPAARWSDGAPVTAADVKWTFDQIMDPRHATGASKVALGIFDSPELLGPRT
                     VRFRARESHWRNLLALGSFEILPRHALAEQDFNRIDFDRPVVSGPYVLDAVREQIEIR
                     MRRRRDWWADARPATRHTMNFDTLIFRYYSDTGNAFEAFKKGLVDVYAIYAARIWANE
                     TIGEKFDKNWIVKCRVRNHNPIGFQGFAMNMRRPPFDDLRVRQALAHLVDRETMNRTM
                     MFNAYFLHRSYFEDLYDDAHPCTNPVFAFDPPRAKALLREAGYRPNPQTGVLEKDGRP
                     LRFTFLTRDGGTDKFLAVCATTFREAGIEMKIERKDFASWMRDMDEYRFDITWAAWGG
                     SLFRDPESMWHSKEADRPSGNNLTGFKDPQVDALIEKQKTLFSITERNAINREIDARV
                     AAAVPYVLLWNINSTRLLYWNRFGVPDTVLAKFGDERSLLGYWWDDADSAAELKAAMS
                     AKDVLPRRPEWVDFDAIFPSPSSP"
     gene            22024..22827
                     /locus_tag="PK576_00970"
     CDS             22024..22827
                     /locus_tag="PK576_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE60194.1"
                     /translation="MFPLQLDRPLVVFDIEATGTSPRSDRIIELAAIRLDPDGTETEG
                     YWLLNPGVPIPIETTAIHGITDEHVKDQPEFKDKAQEILAFFGDSDLAGFNAGRFDIP
                     MLSEEFARVGIPFDALSRRLLDAQRIFHTKEPRDLSAALTFYCGRSHADAHGAEADVR
                     ATLDVLRGQFARYDDLPREMEALDKLLNPIDPFNADRSGRLRWIEDEVVINFGKKKGA
                     KLRDLAKDDPGFLKWIIKNDFPQDTRALCENALKGVFPTPPRPKSAPVQ"
     gene            22928..23563
                     /locus_tag="PK576_00975"
     CDS             22928..23563
                     /locus_tag="PK576_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60195.1"
                     /translation="MKRIIQCSKCEAKLAIFDFGKPINQKCPKCGNAFVVDSEAGKAK
                     APESSAPAPAPAAADKAPTPAPAATSSEPPAAAKPTAPTEAQAPAKTATPDATPDAAP
                     VKPEPAAEPAAKPVDAPAASPDAGQKEITLKKPAPRAAAKPAPAPAPEPDMPELPNSG
                     VSFLHVVVIIGLLVLSIILQVMNKKAAQRSAFDLAERLNRIEAKLRAPQQP"
     gene            23644..23895
                     /locus_tag="PK576_00980"
     CDS             23644..23895
                     /locus_tag="PK576_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE60196.1"
                     /translation="MDTNTLEAKLKEMIVGRLFLNVTAEEIDTNASLIDAYGVDSVCL
                     LELVVGLEEAFGIVIEDSDFDVRNFISVAALRDFVQARL"
     gene            complement(23906..24148)
                     /locus_tag="PK576_00985"
     CDS             complement(23906..24148)
                     /locus_tag="PK576_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60197.1"
                     /translation="MSDTTQQREWGEQPLARILSEAGLTPHDLVTASEVPMTHKMVAR
                     ACKGRWLTPNTRRIVVAALNRATGREYTPSDLFTYG"
     gene            complement(24175..24744)
                     /gene="rfbC"
                     /locus_tag="PK576_00990"
     CDS             complement(24175..24744)
                     /gene="rfbC"
                     /locus_tag="PK576_00990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOE60198.1"
                     /translation="MPVRFEPTELDGVILCVPQVFRDARGFFLETYHAAQYREGGVCD
                     TFVQDNRSRSVRGVLRGLHYQLHKPQAKLVSCLRGEIFDVAVDIRRGSPTFGRWSAAV
                     LTEENHHQLYIPAGFAHGFCVLSEGAEIAYKCSEFYDPGDDRGIRWDDPDVRVDWPIQ
                     DPVLSAKDAAQPLLADAELPEWQPAGVVV"
     gene            complement(24744..26360)
                     /gene="pgi"
                     /locus_tag="PK576_00995"
     CDS             complement(24744..26360)
                     /gene="pgi"
                     /locus_tag="PK576_00995"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOE60199.1"
                     /translation="MNESAWKAVETEVAATRATHLRDWFAKDPKRAERFTVRAAGWTL
                     DYSKNLITPALMRRLVELAEASGLRKEIDAMFSGELINKTENRAVLHTALRNKADCPV
                     YVQGQDVMPKVTAVLDAMSGFAERVRKGKWLGHTGKRIRYVVNLGIGGSDLGPAMACQ
                     ALKPFAKRSLEMHFVSNVDATHLAETLRKVKAEQTLFIVASKTFTTQETMTNAMSARA
                     WLVERLGSETAVAKHFAAVSTDAKRMTEFGIDPENRFGFWDWVGGRYSLPSAVGLPLM
                     IAIGPRLFRKMLKGFHAMDRHFATAPFSRNMPVILGLLGVLYVNGYGAESYAVLPYDQ
                     YLARLAAYLQQLDMESNGKSVDRQGRRVGYSTGPVVWGEPGTNGQHAFYQLIHQGTRL
                     IPCDFIGFCRSHNPLGDHHDKLMANFFAQTEALAFGKTADACRAEGVPETLVPHKTFE
                     GNHPTNTLLADALTPETFGALIALYEHKVFTQGIIWNIFSFDQWGVELGKVLASKVLL
                     ELTASEAPVLRHDASTNTLIARYRAVRGRS"
     gene            complement(26451..28376)
                     /locus_tag="PK576_01000"
     CDS             complement(26451..28376)
                     /locus_tag="PK576_01000"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOE60200.1"
                     /translation="MGRSLVAVWVLVCGACWAQGGAAQDPLKVTREEVRARLERLPAT
                     HPRLFINRADAFKSIASESGTPSVAGVIRDWIIREADAVLPQKPTVRKQEGFRMQGQS
                     QVQKRVGLLATAYLFTGKAAYVERAKEEMLAAAAFSDWNPKHFLDTATMTLGLALGYD
                     WLYDALDPDTRATVAGAIRDQGLKQALARKYFWTEGNNNWTQVCWSGMVAGALAIHDQ
                     EPELALSIIHKAVTSVPYSIRPYAPKGSYPEGPGYWSYGTTYYVIMLDLLTQALGTDF
                     GLYDLPGFPQTGEYINLVTGPSGRFYNYADGGDGRSHLLALWWLARRSGRFDWLTREV
                     ADLREALRLNNPDKTGLRQIALPALLLLWAREIDEHQLTSRMPLHWSSGGHVPLVVLR
                     TAWADPNAGYLATKGGPASQNHGHMDAGSFIYEADGVRWAVDLGAQRYYSIEKLGMSL
                     WNSKQDSDRWKVFRLNSASHNLITIDGQPHVVGGSGSVAHFSDNPERPHAVLDLSPVY
                     AGQAERVWRACALLPSRTGVIQDRLKGVRAGAAVRWAMVTQATGCQTVGQDMVLTHSG
                     KSLTVRVVHPAEVRWRVIDLAEPPNPWDVANPGARMIAFEVTAPQDGRVETRVVLLPG
                     GTAPVEEVPDLITLSGL"
     gene            28486..30486
                     /locus_tag="PK576_01005"
     CDS             28486..30486
                     /locus_tag="PK576_01005"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOE60201.1"
                     /translation="MRFSLSHRPATYIPLLCMLTLSSARLLGADETILWLDTLDLSSM
                     TSAGEPPQAGRTVGGNPFKIARKRFERGVGTHAESWLSLEARGGALAFQAWVGVDDEE
                     LIRGKGSVIFRVYADGRRVADSGIRRAREPAFLLRADLTGAQVVTLHVSDAGDGDARD
                     HADWCEAFFTLKQGASLTPLPAPPTEQMGILTPASPLEPLLRGPRRFGVRPGSPLLFR
                     IPATGEKPIRFAARDLPPGVSLDSTTGLLTGAVTRPGSYAITLAAENARGRTERIWHL
                     EVGERLALTPPLGWNAWNVFAHTVSDAKIRQAAEAMVSSGLADHGWAYINIDDYWQTC
                     PGEREDTALMGPGRDPSGRILPNRRFPDMRGLVQYVHGLGLKIGLYSSPGPLTCGGCV
                     GSLGHEARDAQTIAEWGFDYLKYDWCSYAQIAADDTLAEQMRPFLLMGAALRKQPRDI
                     LFSLCQYGIGHVSAWGDKAGGHCWRTTQDITDTWSSISQIAEAQVGLELFAGPGNWND
                     PDMLVVGTVGWGSPRPSRLTPNEQYTHVSLWCLLSAPLLIGCDLTRLDAFTLNLLTND
                     EVLEVNQDPLGRQAVRVQRTDAQEVWAKPLEDGSLAAGIINRGMLTAPVTLDLTTLGL
                     TGPQQLRDLWRQRDLGTFADASFKTTLPGHGVLLLRITPIKQ"
     gene            complement(30549..32534)
                     /locus_tag="PK576_01010"
     CDS             complement(30549..32534)
                     /locus_tag="PK576_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOE60202.1"
                     /translation="MIEFKKIGVRYGTQSVLEEVSFRVNTGERVGIVGPNGSGKSTLF
                     RLILGEADADAGEVITEGTPRIGHIRQHLAADHEDETLLAYALRGIPGLTEMEEEIHA
                     LQNQLAGAEDEETRNRLLRQLGDVQTEFEHLGGYEMETRVKVSLGGLGFDASEFDKPF
                     KHFSGGWQMRAELSRVLASHPDLLLLDEPSNYLDLPAVEWLQRFLAGYDGTLMLISHD
                     RYLLRTLTSVTVEVDANTATRYNGDLDFYLREREARHANLLAAKANQDRRIEQLERFV
                     DRFKAQASKAAQAQSRMKMIEKIEAVAVKVPKRSLAAGYLRLAPAPHSGAEVARLEHV
                     DFAYDPGRPVLRDVNLSIARGDKIAIVGYNGMGKTTLLRILAGLRVPTAGVCVLGHKV
                     QPGYLSQDFSETVPPDVSVYECAKRCAPDTPEKVIRAQLGAFGFSADDIGKLAGVLSG
                     GEKIRLAFLRLFLAPPNLMLLDEPTTHLDLEGRATLEKALRAYNGTVCLVSHDVEFVR
                     NTVTSVIEITSAGVRRFPGGYTYYREKIATESRNTDNVSRSVPVEQATTDEPVTHARD
                     ARRARAQARSKYQAAIRTLKERVARTEARITDLEAELETLSACLFNPTPETDFAATNK
                     RLKRVQDQLSVFSEEWERDATELDRLQREQQAAVSGT"
     gene            complement(32598..36830)
                     /locus_tag="PK576_01015"
     CDS             complement(32598..36830)
                     /locus_tag="PK576_01015"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE60203.1"
                     /translation="MTRFTKNFSACWRTANLIAVAGVVLTTVAELEPLPNPPEFARRA
                     TLSETMLHNLGALRNWEAAHDARAAAPEGLELGVWHVLGPLKPDSPLTDKIINEYGLI
                     DLTVPLEDRHGRHAWIPCPQFKDHAVCDLTAFRNAGPGDRFIAIRPVTLAERCDLKDV
                     MLDVSLSDGYSQWHPGRNRRDEQGTWPVLDDGYALTIRPGPNQFMMVIRPDRDGYRRF
                     YFRVFRRNTQSPASHSWQRSQRRLALMRRALAEVDAAPHQIRMAEWEALASVWVPQEY
                     NRYRYAWVPGCEAEFASNRFATACNDVLAEIAGRLARMEGLPAQALTPYREMLETWVG
                     QARKPEAAWRRSDPRAAFLKSCALRELMTSAATVISIRLAVEDHIRMFKDAYPQGHAA
                     RRQIDARSREIAEELRAFPHSGPDLPERAARQSVALDALRQTVLLDTPLLAFNRVLLA
                     RGSVSFNSNWGGANQIGDELAILSPVAPSGSVTRVHKGTVSDFDLHWDGRRVLFSDRK
                     ILWELDLQAPDDPPRRITDAPPDVWHYDGCYLPDGGLLAVCNACYQAVPCTGEANVGN
                     LHRMDADGRNERRIAFDQDHNWNPTVMHDGRVLYTRWEYADIPHYFSRFLMRMNPDGS
                     GQMEYYGSGSYWPNAMYWPRPIPGDPHRVVCIVSGHHGVSRAGEVILLDPRQGRHEAS
                     GVVQRLPGYGQTVEPVIKDQLVSDVWPRYAAPYPLAEGPAALGAGRYFLVCRQRNAFS
                     GWDLCLIDIFDNVTPIKHFETLPNGQFVNYMSPVPLRARPQPPVIPSQCNPSRKDAVI
                     YLSDIYSGQGLRGYPRGSIKALRVGTHHYRYFGNGFTYAHAFEGGWDVKRILGTVPVH
                     EDGSAFFRVPANTPIFVQPLDAEGKAQQQMRSWYTAMPGETAACIGCHEPQNSVPPVQ
                     PNLAARGKPVAITPWQGPVRGFSFEHEIQPILSRRCVGCHQDKPLKSGSGGQTTMEDF
                     RDKRLQPHDPSLIPPKCEWWWWLGSANKHLKEPPLCFSPAYMRLQKYVRRAGLEADLH
                     LLAPAEFEADTSELVQRLKKGHHGVTLTQEEWEALYTWIDFNVPYCGTWRESNLPPNE
                     KHVRMRAEYKKRYAGLEDRDEEGVPLPPIPRFEPPSAPVAKTTAAPQVDGWPMNAGQA
                     TAAQRAAGEPELTLDLGGGISMRFALIPAGTFVMGRADGFADEAPRHAVAIDKPFYLG
                     VWEVTCEQYARFNARHESGYIEGRNKDRTTRGTFIGSPRHPVVRISWREAMAFCAWLS
                     RVSGRTCTLPTEAQWEWACRAGSDADFAFGPALANHQANLADEGIRDWNYGRAQTGYQ
                     DGARFSAPVGRYKPNVWGLYDMHGNVAEWTRSAYRPYPCDPSGDEDEAGLKVVRGGSW
                     NDTMAFAGSASRWRYAAWQPVYQVGFRVLVVP"
     gene            complement(36855..38975)
                     /locus_tag="PK576_01020"
     CDS             complement(36855..38975)
                     /locus_tag="PK576_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60204.1"
                     /translation="MCRGGVAGLCFLLGVCGALAANVTLHVSPQGKDTWSGTLAAPNA
                     AKTDGPLASLGGARDALRRLRDAGTLTPKQAVTVRFQIGRYEQRTPVVFTKEDSGSPE
                     APVVFEAAPNGQVRITGGVALWKMNTLTDTNLLRRLPEAARGKVQVISLPAQGVYEYG
                     VEPVGSSAGPVSGIQLIYGDEVMRLARWPNEGFVTIADTPDGKQGNTFTYAEENPSRW
                     IDEIDPRGQGYWAHDWAACAIAFERIDPATKTITQKVPGSHYGFRKGGHWFGYNLLCE
                     LDKPGEYYVDRLDGKLYFWPPGKSANTRAEVSVGTRLIQLDDVAHMQFRGLTFENCRG
                     EAILISQGESVNVIGCTMRNIGHRAVTIRGGKTHRIAGCEMTRLGDGGIQVSGGNERS
                     LERCGHVVDNNHIHDYARFSMTYGAAVQLYGCGIRVSHNLIHNGPHVAVLFSGREQVM
                     EYNEIHSICLSSGEMGAFYAGRDWTLVGNVIHGNYIHDIYNPRPQRNRAIMLDDGAAG
                     ITMTQNLIVRVAEGISLSAVGNVIDNNVFVDCHPAISGWQTYETPESVTPPKGVHAQM
                     PERLFATPVEDPLWKERYPWLLAFRNAVRDKKLRPPETRTRVERNVIWGGPSVWLAHY
                     KKYPRTVHSWLVGENNLAGADPLFIDAAKGDYRLQSASPAFKLGFRPLPIEKMGLYAS
                     PERAVWPVRHEVTQVCTNLTYVKP"
     gene            complement(39219..39701)
                     /gene="fabZ"
                     /locus_tag="PK576_01025"
     CDS             complement(39219..39701)
                     /gene="fabZ"
                     /locus_tag="PK576_01025"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOE60205.1"
                     /translation="MSNEEKITWQQLVASGKEYPGSIEELLPHRKPFLFVDRLLYFDQ
                     QNYIGEMTYGEDMWFFQGHFPGYPVVPGVILIETMAQCGGAGLVASGLIQNGVFLLVG
                     VDNAKFRRQVRPGDTLRMEVENRRVNKRMAVQHGKAYVGEELAAEADFRCVMRPATDN
                     "
     gene            complement(39767..41011)
                     /gene="fabF"
                     /locus_tag="PK576_01030"
     CDS             complement(39767..41011)
                     /gene="fabF"
                     /locus_tag="PK576_01030"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOE60206.1"
                     /translation="MSGKTKVVITGLGVISPVGNTVGEMWDNIRNGVSGIARTTRFDP
                     SRVDSKISGEVKQFDITQYVDPKEARRMALFTQYALAAARQAWQDAGLADGGADPERV
                     GVFVGNGIGGKEVDDEAYQVLFNRGPGRLSPMLIPKLIANEAAGNISIALKAQAAVHT
                     LVTACASGTDALGVAMDMIRAGRADVMIAGGTEATVTEYCVGGFCSMKALSTKYNDTP
                     EKACRPFDAGRDGFIMGEGSAILILEGEAHARARGARIYAELAGYGASGDAYHLTAPD
                     PEAAGAIRAIRLALKDAGLAPDEIDYVNAHGTSTPLNDPMETKAVKTVFGDHARKLKM
                     SSTKSMTGHMLGAAGAIEALICTLAIRDGFYPPTINYTTPDPACDLDVVPNQGVNGPI
                     RAALSTSLGFGGHNGVLVLKSV"
     gene            complement(41048..42064)
                     /locus_tag="PK576_01035"
     CDS             complement(41048..42064)
                     /locus_tag="PK576_01035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE60207.1"
                     /translation="MFVHITGTGSYLPTRVVTNAELAEKLDTTDEWIFSHTGIRSRHL
                     GADDETTSSMAIQAGRAALEAAGVRAEELGMIVVATSTSDYQAFPSTACLVQHALGAT
                     QAAAFDLQAACTGFIYALWMVYGLMQVDPRPTLVIGAEMMSRIVDWTDRNSCILFGDA
                     AGAVVLQTSGQSGGIRDAILRADGSGADLLYREGGARQLATGPWMHNTLFMKGRPVFN
                     FAVKVFDEILCGLLERSGHTFADLACVIPHQANARIVEAVARRMKVPLETFHMNVATT
                     GNTSAASVPVALDDAVRSGKLTDGGLVVLIGFGGGLTYGGILLQWHDVAGGVPPQRPH
                     PESA"
     gene            complement(42154..42330)
                     /locus_tag="PK576_01040"
     CDS             complement(42154..42330)
                     /locus_tag="PK576_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60208.1"
                     /translation="MKNLLFHRGAQLLVDPLVAGFPALALQTFSKLLSHNERSLYQAG
                     FSLSTARFESVSFS"
     gene            42334..42407
                     /locus_tag="PK576_01045"
     tRNA            42334..42407
                     /locus_tag="PK576_01045"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42366..42368,aa:Cys,seq:gca)
BASE COUNT         8188 a        14279 c        12051 g         7979 t
ORIGIN      
        1 ggtcgaatgc cgcaggtcgc agaacgatgt tcacgcgaat gtgaacgggc acgagaacga
       61 ggacggggcg tccctattga ctgtcgcacg atggtcaaat ctcaagcgtc gcaggtcgaa
      121 tgccgcaggt cgcagaacga cgttcgcgcg aacgtgaacg ggtacgagaa cgaggacggg
      181 ggcgtccctg tcgactgtcg cacgatggtc aaatctcgag cgtcgcaggt cgaatgccgc
      241 aggtcgcaga acgatgttca cgcgaatgtg aacgggcacg aggacgagga cggggaagct
      301 atggattttc gattctggct cgtgctgctc atgcgggggt ctgcccactt ctcatgagat
      361 gtggacaagg gcactgcaaa agaggcgcgg catgaacggg attcttacct tacacagtga
      421 atgtggaccc ttacccttac aggatgttgg gtacatcgga catctacagg atgttgggta
      481 catcggacat cgttcgggga tcgtttttcg ttgcccttcc ggatcgtcgc cgcttacaag
      541 atggtggaca catcagacgc cgttcgggga gttcccgtac cggacgtggt ctaggtgatt
      601 gaacggcggg cgtgcgggag aagaaactat tcgaattccc ccgcatcgta tttggcccag
      661 aattccgacg gtgaccacat cgtgccacgt gagaatccga cgccgatgat gcgcagcgtc
      721 ggcatggccc gtaccgcctc gatccccttt ttaatccgtc gcggcgtaaa caagaactgc
      781 cgcaactgaa gatcggccaa cggtgtcagg tccgacaccg cactgtcggt catgttaatg
      841 aagtacagcg gcaaaccgcg aagcggctcc acgtccgtga ccggagtgcc ggacatatag
      901 aggaaccgca gtgctgaccc tttcagggga cgcaaatcgc acaccttcgt cccaggaata
      961 tggagccgtt ccaacggcat accgaccaag ggagagaggt cggtgatccg cgtccacccc
     1021 atgtgcaact cttttaaaga caaccccacc acatccgata gattcgtgac ggtggtatag
     1081 ttgatgaaca accgcgcaag cggcattccc ttgagcgggg agagatccgg aatatccgtg
     1141 acctccatat gcagtctgtt taacggcatc ccccgcaaag gctccagact tctgaccgac
     1201 gtctgaccta tctccaaatc gaccagcggc atcccctgca aaggcgaaag gtcggcaacc
     1261 ggacaacatc ggtgaagatt taaattcgtg atggccaacc ccttcagcgc agagaggtcg
     1321 gacacacggg aactccggat atccaatttc ttgagcggca tcgcccggag cggcgacaaa
     1381 tcttccactt cagagtattg cgcgcccagc tcgacgacgc gcaatccagc gagcggacgc
     1441 agatcacgcg tcgccacccg ttttttgtac gcgccgccga gatcgacccg ccaaccatcg
     1501 gccatatcgc cggaaacccg ttcacagaca aatccggcct gccgtaaccg cgcgatccgg
     1561 tcctcctcgg acatcccctt cagttcatgt cccgcagtcg tctcgggcgc atgcgttttc
     1621 tccgtcaggc atcccacgcc acagacacat gcgacacctg caacgacgat accccatcct
     1681 atgcgccgcc gccaatcaaa tccccgatcc cccatcccgt gacctccgcc atcctacagt
     1741 ttgaagagtt cgcggtaacg cggtgcccag taggtgagga tgatctccgc gcccgcgcgc
     1801 cgaatggcgg tcagcgattc ccgcgccatc gcgtcccgat cgccccaccc cctctcagca
     1861 gcggcgatca gcatgctgta ttcgccactg acgttgtaag ccgcgagcgg caggtcggtc
     1921 cgctcgcgta gccgggacag aacatccaga taaaacaagg cgggcttgac catcaggata
     1981 tccgccccct cgtcttcatc gaaatccgat tcgcgcagcg ccgcgcgcag gttaccgccg
     2041 gaggcctgat agccctgccg gtcacccgaa gacggagagg agcgttccgc ctcgcggaac
     2101 ggcccgtaca gagacgacgc gaatttcgtc gagtacgcca tcagcagggt ctggtcgaac
     2161 ccgttctcct ccagagccgc ccggatcacc gcgacctggc cgtccatcat ggcgctgggc
     2221 gccaccacat ccgcaccggc cgcggcatgc gaaaccgcca cacgggcgag cagaccgttc
     2281 gcggcatcat tgtccaccga tccgtccggc gcgagcggtc cgcaatgacc atgcgcggta
     2341 taggcgcaca ggcagacgtc cgtcatcacc accagttcgg gataggcgcg ccggatcgcc
     2401 ggaaccgcct gctgcaccac gccccgctca tcaaacgcac cgctgccgcc ctcgtctttg
     2461 tcctgctcgg tctgcccgaa cagcaacacc ccgcccaccc cttgcgccgc cacgggttcg
     2521 agcgcacgca acagatgatc gacactcatg cggttctgtc ccggcatcgc gtcgatcggc
     2581 tcacaccggt tatgtccctc caccacaaac accggccaaa tcagggatga cgcgggcggc
     2641 acgggatcgt cgaacagcct acgaatcgcc ggggtacgcc gcaagcggcg caggcggact
     2701 tcgggaaaac gcatgcggac cttcctttca atccggaatg aacagtatct ggcccgcacg
     2761 gattgcggac ggattggtga tcttgttggc cttcatgatc gactccatgc tctttccgta
     2821 tccgcgggcg atttgggaga gggtctgccc tcgttccact ttgtgctcat acccggaacc
     2881 gcttcgggca ggtgaagcgg aggcagaggg tgccgcgcgt tccgcggcag cacgggccgc
     2941 ccggatttcc gcctgctggc gtgaagcgat cgtctcaata cgtccggcca gatcgtcggt
     3001 gatctctttg cgcaacccct cgcgctcgac gcggactcgc tgcaactcct gccgcagcgc
     3061 gcccatctcc tcgccggaac gctgcagggc cgcaagctgc ccttccagcc gcgtcagcct
     3121 cgcatcaagc acttcctgat tgcggctgac cgcagccacc tgttccgcaa gctgccggaa
     3181 ctccagattc atctgctgcg tgctcgtggc gatccgcgcc accgttggat cctgacgcgg
     3241 ctgcgcctcg aacatcgtcc cttcaaacat cggggcctga catcctgccg cacatccgag
     3301 tcccgctgcc aacagcagca acacacgttc tcttcttcgc atccctcctc ctttcccgtc
     3361 cgtcacaggc agggcaagaa ataaaacaac agccagaccg cagaaagaag cgcgaggaac
     3421 acgagaaacg cgcggcggcg cgcctcccgg tcatcgtccg ccgaagtgcg gcgcaattgc
     3481 cgcaatccgc ccgccgcaat gtaatggaac agccggttac ggccgcggct cctgagggcc
     3541 ggacgccgac gcttaccgaa aaaaaagctg ttccccgccg catcctcccg cacatccaga
     3601 cgccacgatg cgggtgtccg cctgacatcc gcatcgacga acacactgtt cggaccctgc
     3661 ttgagcacac gtctaccggt ggccggtggc ataccttccc atccccctct ctcaaggcac
     3721 ttcgataatc tgtcccgccc ggatgcgtcc gtccgcatca atccgtccgc ggttcgcgtc
     3781 acgaatccgt ttccactggg tcgagtcccc gtaaaacctc tccgcaaccc ggaaaagcgt
     3841 atccccgggc tgcaccacat aggtccggtg cgcccccgct ttccgggttc ttttctcctt
     3901 gcggccgtcc tgcccgaaca gcgagagcgc ggacagcgca tccttgtccg gcgtcacgtc
     3961 gatcggcggc ggcggcgcga ccgtttgggc ggcgggcgcc gaatcgcccg atccgctggc
     4021 aagccgcgac tgctctttgc gggcggcggg caccgaatcg tccgatccgc cggtaagccg
     4081 cgactgcact ttccggagga ccgattcggt tgtcggcgcg tccaccaccg gcttgcggat
     4141 cggctgcggc ttctccgact gcgcggccag ttccgccgcc tcctcgcgca gcgcgcgcaa
     4201 cgtgcgggca tcggtggtcc gcttcgattc cctcgtgtcc gccgcatcac gagccgcttg
     4261 ggccgcctgc cctgcggccg cttccggcgc ggccacctcc tccacacgca tcttgcgcaa
     4321 caagtcacgc aaccgctcgt tctccgcctg caagcgcgtc cgttcctgct ctgccgcggt
     4381 cttggactca acgagcatgg atttctcacc ctccagcgtg gtgatgagcc tgttgaggcg
     4441 gtcattctcc ttgagcaggt gcgcctgcga gatcccctgc accgaatccc cgactttccg
     4501 caggatttgg ggggccagca attgttcggc aatgcggatc cggtcttggg ccagcgtgct
     4561 cttttcggct tccggcctca gatagagata ttgattatag tggtagatcg cccccacata
     4621 atcctcggca tgatcatgaa gcagcgtggc caactggaag tgagccgaga aggaacgggg
     4681 ctcttcgatc aacacccggt tgtaaagccg gatcgcctcc ttcagatcgc ccgactgctc
     4741 ggcggcgaag gcctttcgat agagaggcga cccgcgttcc gcactctcgg cctcctgcac
     4801 gccagacttg ccgcaccccg cccctgaaag gagcaggagc gacccccata cgataatccc
     4861 gtatcgaacc attacaaaag cccttccgga aagagatgcg accccgcatt tttgatttgg
     4921 tttctgcgtc agaccgacat atcatgtctg cactttggtt tttaggccgc cgcgcctcaa
     4981 acacagaatg catgcgtgat cgtttattgt actgatctcc tgaaaaatga ccactgaaaa
     5041 cacgaaaaaa ctttgggatt cggcccgcgc cgttctgggc gggaatggtt gtctgagcgt
     5101 tatcatgccg gtttacggcc tggaaaaaat catcgggcag aacattcagg ctgtctgcga
     5161 tctgctgcgc gacgagattc cgtttgaggt gctcccggtg gatgacggca gccgggacgg
     5221 cacggccgag tccatccggc aggcggcgag ccgggatccg gagcatgtgc gccccgtctt
     5281 cgtccaagtc aatgcgggca agggcaacgc cctcaaatgc gggttcgcct cttcccgcgg
     5341 ttcgcacatc ctgctgctgg acggcgatct ggatttatcg cccgcgcgga tcaccacgtt
     5401 ctttgaggtc atggcgcata accgcgcgga catcgtgatc ggctccaagc gtcatcccga
     5461 atccgtgatc gactaccctt ggcaccgacg cctcgccagc gtgatctatt acacgctggt
     5521 gcgcctgctg gtgggactgc cggtcacgga tacgcagacc ggcatgaaac tcttccgccg
     5581 tgaggccctc ggctgggctt tcgaccgtat gctggtgaag gcgttcgcct ttgacttgga
     5641 gattctctcg atcgcccatg cccgcgggtt caaggtggct gaagcgccga tcgaaatgca
     5701 tttcggcaac aaagtggggt gtctctcttg gtccaacgtc cggcaggtga tgaacgacac
     5761 gctcgcgatt ttctaccgcc tgcgaatcct gcggtattac ggcagtgtcg aggtggcgcc
     5821 gcccgcctcc cgtcccatga cggtctccgt ggtcatcgcc tgtccaggcc caagcccgta
     5881 tctcacggag tgcctcgaag cgctggcgtt gcagacccac cgcgcgttcg aagtgctcgt
     5941 actccccgat gagccgctta cgttgccaga gtaccccttc acgctgcacg tgctgcccac
     6001 cggcaagacg cggcccgcag aaaagcgcaa taccggcctg tggcgggcgc agggtgaaat
     6061 cgtggcgttc cttgatgacg atgcctaccc cgtccccgcc tggctggagc atgccgtcaa
     6121 atacttcacg ctaccggatg tcggcggtgt gggcggacct ggcgtcacac caccccacga
     6181 cccattcctg gcccaagcga gtggacgtgt ctacgccaac cgcctggtct ccggaaacta
     6241 ccgttaccgc tatctcgggg accgtgtgcg gccgcggatc gacgacttcc ccagctgcaa
     6301 cctcttcgtc cgcacggagg tgctcaaggc catcggcggt taccgcaccg atttctggcc
     6361 cggggaggac acgctcttgt gcgccgacgt cgtgttcgga caggggaaac ggatcgtgta
     6421 cgacccgtgg gttcaggtgt tccaccaccg tcgtccgctc ttcggacccc acctgcggca
     6481 aatcggacgc tacgcgctgc accgcggcca ttttgccaag cgtttcccca acacctcgct
     6541 gcacctaagc tatttgctgc cctcgctgtt cgtcgccggc gtgatcgcgg gtgcgatcct
     6601 gtccgtgttt taccccccgc tgcgtatccc gtacatcgcg gtcctggcca cctacgcggc
     6661 actgaccctg ctgtcgagtc tgaccctgca tccgggactc tggtttgtca cctggctcgg
     6721 cgtgatgggc acacatctgg tttacggcgt gcgcttcgcc caaggactgc tgacccgccg
     6781 gatgccctgc gaagccggag ccttcgacca cccgtctgaa aaaccaagcg gggcgacatg
     6841 attttgccac ggaaaaaacg acgcaacacg cggcaacgga tcccgcaggt cgcgctgttg
     6901 ctggaatcgt cgcatgagat ctcgcgcggc atgttgcgcg gcattctcga atacgttcat
     6961 ctctacggcc cttgggctct gcatctgaac tccggaggcg ccaatgacca gaagttgccg
     7021 gatcgcgcgg cctggcgcgg ctcgggcatc atcgcgcgca tccccaatcc ccacgtggcc
     7081 gctgatatcg tcgcggcacg cctccccacc gtgttgatcg atccggtcga tgcgtacctc
     7141 aacccccgcc acccgctcgc gcgctgctgc cgcgtgagct gcgattccgc cgccgtggcc
     7201 cggtgtgccg cggactacta tctggccaac gggttcacgc ggttcgcctt tgtgggcgaa
     7261 ccctctggtc tcaactggtc cctgtggcgt cagaaggcgt tcgtgaaccg tatcgcggag
     7321 gccggattcg cttgcgacgt ctatccgccc ccctcggagg aggaacgtgc cacatgggaa
     7381 ctggaccgca aacgcatgtg ccgctggctg gccaaactac ccaaaccttt ggccgtgttc
     7441 gccgccaatg acgcgcgcgg caggcaggtg ctcaatgcct gtctggccgc cgatcttccg
     7501 gtgccgtacg aagtggccgt gctgggcgtc aacaacgacg tcatgatctg cgaaaccagt
     7561 ctgccgccgc tctccagcgt ggcggtggat atggaacggg ccggattcgc cgccgctgaa
     7621 ctgctggacc aactcatgcg caaaaccctg aggaaacagc gttgcgtaac gttcgcgccg
     7681 cagaaggtgg tggcgcgcgc ctccacccgg cgcttgcagg tgaatgaccg cctcgtgatc
     7741 cgcgcccttg agttcatccg catcaacgcg ggactgaatg tccgcgtgtc ggatgtcgcg
     7801 gaccacgtgg gtgtcacgca gcgttgggtc gagaagcgtt tcgcccagac gctgaaccgt
     7861 tcggtgatgg atgagattca gcgcgtgcgc atggaaaccg tccgctcgct cgtgaccaag
     7921 agcaccctga ccttttccgc gatcgcgcag cgttgcgggt tcacgaacgc caaccatctc
     7981 ggcgtactct tccgccacac gttcggcacc accatgagtg attaccgcca tcagcatgcg
     8041 ccgccgacgc tcaccgcgcg accgcgctga cgcgggtcgt gttctctggc ttcgggtttg
     8101 ggggttcagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcgaccgc
     8221 gctgacgcgg gtcgtgttct ctggcttcgg gtttgggggt tcagtaaccg cacccgtttt
     8281 cgggcaggga cacctccccg agatgtccgc aagtcagggc ttcagcgccc gcttggccgt
     8341 gccgtacgca tcccaaccgt ccggcgcgaa atacaactcg aggaagcggc cgatgagctg
     8401 acttccgcgg gcgttggcgt ggaccgcatc tgacttgaag gcgccgaggg gttgtttgga
     8461 agcggtgagg tggcgcatcc acggttgcgt gatattcacg tattcgacgc gttcctcgcc
     8521 ggccatcttg aacagacggt cccgatatcc ggttcccgcc gggtccggat ctgccgcgtt
     8581 cttcggatca tacggatcca gatccttggc ccacggcccg ccgtagacgt cggtcatgac
     8641 caccacctcc acctccggca aggccgcgcg cgtctggcgg atcaccgagc ggatcgcctc
     8701 cacatccccc ttctggctga tgccgccgat gaccagcaga tccggcttgt ggcgcagcac
     8761 gtagttctgc aagtgttccg gctcttgata gtaccagcag cccgtgctgc cgcgtaccga
     8821 cacgatcttc tcgatccggc tgccgggata cgcgcgttcg atcagccgct cgaagtgcga
     8881 actctggatg ttgttgatga tgctgtcgcc cagcatgacc atgcgcaccg taccacccga
     8941 gcgtaatcgc tccatggtct tggggatccg gctccagcgt cccggcggcg gcctgtaggc
     9001 gatcggctcc aactgaagaa aaagcgtgtc cgccaacgcg accggcgaaa gggagcccat
     9061 ggggctcgcg tcccgcccgt tgacggtgag ccagccgatc cgcgcaaccc ggcctgtgct
     9121 gccggacggc acggcttccg ccacccgtat ccgcacggca tgccgcgtat ccggaagctc
     9181 gtccgcgaac atgcggtgga aaagatgata cgcgttgtcg cgggcctgcg ggtcgaacac
     9241 ggcgagccgc tgccacggac catcatcgat cgcgacctcc agatcgcctg aatccggacc
     9301 ggtcacaccg tacaacccgg ccgccgtgcc ggtgaagtac agggtcatca ccggccccgg
     9361 cttctcgcaa accgccacat ggaaaatcgc gtccacgggg ctccattgcc aacccaacca
     9421 ccctgcggga tccagatcgc cccgttcgta attgaccagc gtggcccgat tccacaatgc
     9481 cggcgtcaac ggtgacggac aggcgtgccg gaccggttgc gcttgagcgg gttgcgccgc
     9541 gcaccaggaa aagagcgcgc cgagttcgtc gcgcaacagc gcatcccact cttcctgcgc
     9601 acccttccat tccggtttac gcgccgccgc tttcgcgaga ttcacactcg gaatcccgta
     9661 atgctccgcg atctcctcat accagcggat cacgtccggc gtccggcccg cccgatacgc
     9721 cgccacaaaa gatgcctccg gctgatggag catcaacata tcccgggccg ggttccgctg
     9781 ccgggtccgg tgaaccagtc cctccagagc cgcgcggacc tgacgctccg tctgtccgat
     9841 atcctgatcc gagagatcga tcaacgttaa cgcgacattc ggccagctcg tttccggaga
     9901 cacagaagga ccgtcggaca tagcccggaa cgtttcaatg accgaggagc aattgtcggg
     9961 ataggcgcgg ccccatgaga tgaaggggaa gacacctttc gcacccggaa acgcccggac
    10021 caccagcggc ggcacgcgat tcctgacttg tccccgcggc gtttgggtcg tgcacccggc
    10081 atagaatacg catccgttct gcgttttggc gcgccagatg cggctcgcgt tcggcaatcc
    10141 ctgacgcgtg acgcacaccg gatcttcggc accgcatacc gccagagcca cgagcccgca
    10201 catcctgatt atcatccttt ttttccgcat tgtttgcctc cttttgctct cgtccacccg
    10261 atctggtttc cggttgtgta gcatggcctc cggcgcgtcg cctgtcaatc ccggaatccg
    10321 gtctgcagga cgccttttga acgcgcggtt ttcgtgttga ccccgcatgc gacttcactt
    10381 acccttaaac gacttttccg ggaccggaag gtcacgttgt tttccttcgc gacgtattgg
    10441 atttcttttt cccatgaatg aaccctctct cacgcttctg gccatcgcgg atctgcattt
    10501 caccggactg gcgcgtcaaa ccgcccaacc gccgcaactg cgcggcgagt tggcgcaggt
    10561 gttgctgaga aaagtgttcg cacgtttgac acacatgggg gtcaagccgg acctcaccgt
    10621 gctgctcggt gatttgacgg aagacggcac agatcctcaa gcgaagctgg acctgatctc
    10681 gctgaatggc gaactgacgc gcagcggcat cccggttctg gcactgccgg gcaatcacga
    10741 catcgcgccg gccgcctttc accgcctgct cggttgtgag cccggcctcc atctgttcgg
    10801 tggatacggc ttggtgcttt ttgatgacgt gtacggtgag gaccgcacct gtgcgcggac
    10861 ggaagacgcg ctcgcgatgc tccggcagat cgccgccgac catccggatc tgccgctgat
    10921 cgcgttgcag catccgccga tctatccggc gatcgattca cactatccgt accgtcccac
    10981 caacgccgcc gaaatcctcg acacgtttga gaagtcgaag gtggtcctct ctttgagcgg
    11041 tcattaccac aaaggccaaa aggcccgcat gcatgagggt gtgctctacc ataccgtgcc
    11101 ctctctctcg gaagcgccgt tccggttcag cctgatccgc ctgaccggcc accgcgtgga
    11161 aatcgaggaa atccaactcg cgatgggctt gcccaacctt atcgatgtcc attgtcacac
    11221 ggagtacgcc tattgcggca cgacggtcaa caccgcgtcc tgcattgccc tctcgcaggc
    11281 gttgggcgtg acggggctct gcatcacgga gcacgccttc caactctatt tcgacaaaaa
    11341 agaggcgatg gggttcgcgt ggcaacgcaa cccggagatg gctgaagcgg tttgggcgac
    11401 accggagcgc aagcgcatgg ccgcatacaa acggttcgcg cagaccctgc gttcgccctt
    11461 cgtgaaggtc ggcctcgaag tggacctcta tgaccacggc aagttgctgc tcgccccgga
    11521 ggatgccgaa agcgaatggg acctgctggt cggcgcgatt cacctcatcg aagattatgt
    11581 ccccggacgg accagccaga aagaggccga gcgtctcttc ctgcgcgacg tggaacttct
    11641 gaccggacac ccgatccaag tgctggcgca ccctttccgt ttcttttcac gcaaccgtct
    11701 ggcatgcccc acgcacctgt atgcggcggt ggctgatctg ctggccgaca ccggcgtggc
    11761 ggcggaggtg aacttccaca cgcatcaacc ggacccggat ttcctgcgca tctgcgtcga
    11821 aaaaggtgtg cggctggcgc tggcgtccga cacgcacgat ctggccgagg cgggcgagtt
    11881 ctggccgcat gtgcaggtgc tgaaacaggc gggcgtgacc ccgaggagct ttcccgaaac
    11941 gctcttccgt ttcacgtgac acgcggggct tactacagat aaagcagatc gggcgcgagt
    12001 ttcaacacat ggttgcggca ataggtgtaa atcttgttcg ctgacgtgcc ggtcatgcgc
    12061 cgcacgatcg cggccatttt gcgggcctcc tcaattgaga cctggctgat gaccttcttg
    12121 accagcggga tcagattcgg cgacatgctc aacgcgtcca cccccaaccc gatcagcagc
    12181 acggccatga cgggatccgc ggccgtctca ccgcagaccg acaccggaat cccgcgcgcg
    12241 cggccggcgc agacggtcag atcgatcaga cggaggactg ccggatgggt tggctggtag
    12301 agtcgggcaa ccgtctcatt cacccggtcc accgccaacg tgtactgaat caggtcattg
    12361 gtgccgatcg acagaaactc gcactccgcg gccaaggcgt cggcgatcag cgccgctgcg
    12421 ggggtctcaa tcatcacgcc gcgccggatc gtctcgttga agggaatccc ctcagcccgc
    12481 aacgcactca tgcaggcacg cacttcgagg ttcgcggcgc gcacctcctc gatggtcgcc
    12541 accatgggat acatgagttg cacgttccca tgcgcgctcg cgcgcaaaat cgcgcgcaac
    12601 tgccgccgga agacctcggg atggctgagc agataccgga tcgagcggtt gccgaggaaa
    12661 gggttggcct ccttatacaa cgcacccgta tagtgcggca tcttgtcgcc gcccaaatcc
    12721 agcacacgga tcgtgaccgg ttgcccgtcg gggaacacgc gggccacatt cgtgtaggcg
    12781 cgggtctgct cctcttcggt cggctcctga tcgtaggtca gccacaggta ttcggaacgg
    12841 tagagaccga ccccctcggc ccccaccgca tagagatcgg taagcggcgt gccgggatcg
    12901 atattcgcca gcatcggaat ccggtgggcg tcggaagtgg cgccgggcag gcattttccc
    12961 tcctcgatct tctcgtccag cgtccgggaa cgggccacca ttttttcaaa agcgaggcgc
    13021 tcctgttggc ccgggttgag gataaccttg ccgctggtcc cgtccagcag cagcgcatcg
    13081 ccggtccgca cctgttcaga gatcgaaccg agtccgacca ccgccgggat acccagcgca
    13141 cgcgccaaca gcgatacgtg ggaggtgttg ctgccgcggt ctgtcgcaaa tccgaggatt
    13201 aagttccgcg gcagcgaaat cgtctcggac ggggagagtt catccgccac cacgatgcag
    13261 ggcttctcca cgcgcaccgc cagagggtcg gccccgccca aaaggttccg cagaatccgc
    13321 ttcgcgatgt ccccgatgtc ttgggaacgc tccttgagat acgcatcgtc catggcggcg
    13381 aagatggagg cataccgttc cgccacactg ttcaccgccg actccgcgtt cacctgtttc
    13441 gtggtgatct tctccttaca ggaaccgatg aaagaagggt cgtcgagaat catcagatgc
    13501 ccgtcgaaga gggtggcctc gtctccgcga atccgtttgc tgagttcaag cgcgagcgaa
    13561 cggatctgcg cccgcgtcaa cgtgaacgca tcggccaagc gcgccagttc atggggcacc
    13621 tgttcgggac ggatcccgta ttccggcacc tgctcggtcg tcgtggttcg gaacagaaaa
    13681 gccggccccg cgacaaagcc gcgcgaggcg gccaggccgg tcagaacacg cgcctcgttg
    13741 cgcgagggcg tcagatgatc cgaatcattg ctcatcaggg atatcgaatt tgcgggcaac
    13801 cagcgactcc aactcatcga gcgcctcacg cctctgggga ccgtgggccg ttacgcgcag
    13861 gaccgtgcca cagaccgcct ccaacgtcat caacgccatg atgctcttgc cggacacgac
    13921 attcccgtct ttctccacct caacctcggc gttaaagcgc gaggccactt tggcgaaaag
    13981 tccggccgga cgaacatgca tgccgtattt gttcagcagg acaaactccc gtgtctccgc
    14041 atcggtatcc ttttttccca cgatctaccc ctccccgccg gcatgatgcc gtttgatccg
    14101 cgcatccagt tcgcgaaccg caacaaaccc cgaaagccga agcttatgct cctgcgccgc
    14161 ggtctccacc agattgacca gatcccgtcc cggtgcgacc gggatcacca actgcggcac
    14221 ggtgattccg agaaacgtcc gccgcatcga ctccgtacct gtgcggtcga gttcggcatc
    14281 cgcctcgccc tgccgcttga gcgtcaccac cagatccagt tgtttctcac cgcgcaccgc
    14341 cgccacaccg aagatcgagg gcacatggat gatgccgata ccacggatct ccatgtaata
    14401 ctgcgtcacc tccatagcgg acccgatcag atggttgttg ctgtcacgcc gcagcatcgt
    14461 caggtcatcg gccaccagcg cgtggccccg tttgaccaga cccagcgcag tctcgctttt
    14521 gcccaaaccg gccgcgccct cgatgagcac gccgaccccg gccacctcca gcatcgtgcc
    14581 gtgcacttta cagcgcggcg cagacagacc ctccagcacg aaggtggcgg catggataaa
    14641 aaaccgcgtc accatcccgg agcgcaacac cgccaccccg tcgcgctctg acacgcgcaa
    14701 cacttcggga aagacccgtt tgccgcgggt gaacaccatg caggggatct tgtgggcgaa
    14761 caatttttca aagcgctgca cacgcagcgc ctccggcagg gaggagagat aggcatgctc
    14821 cgccatcccc accacctgaa tgtgcttccg cgcgaaatgc ccgaaaaaac cggacagtgc
    14881 caaaccgggg cgatgggtca cgggctcgac aatcacccgt tcgagaccgc gcgccccgga
    14941 gaccagttcc agattcaacc ggtcacggcc gctcgtgaga aactggccga ccgtgacctt
    15001 cgattgccgg accttgctct gtctgctttt ggccattaca ctttggcggc tttccggaca
    15061 ttcgccttaa tgcgcttaga caacgttttc cgcaactgtt tctcgacacg tgcggccgcg
    15121 acatcaatca ccgctctcgc gctggacgca tgccctttga caccgaccgc cgtcagcccc
    15181 ttctgctgca cgacgaattc ggcctcatac aggttacgct gcacatcgat gaccacacgc
    15241 acgctctcca cccgcggaaa cgtctcgaac agtttgagcg cgcgcttctc ggcatacttt
    15301 ttcacatccg gggaggcctg cttgtttcgg accgtcactt caattaccat aaaccctctt
    15361 cctttccttt gtgcccgccg tcgcgcaggc gttttgtttt gtcacatcct aaagtgtttg
    15421 ccgagataca accgacgcgc cgtctcgtcc tgcacaagga acgaactcgg gccctcacat
    15481 aaaatctggc catcatacac gaggtaggcg cggtccacaa tcgagagcgt ctcgcgcacg
    15541 ttgtgatccg tcacgataat gccgagcccc ttgtcgcgca gccctctcac aatctcctgg
    15601 atatcatgca ccgccaacgg gtccaccccg ctgaacggct cgtccagcat cagaatcgca
    15661 ggcttgcgca ccagcgcgcg cgcgatctcg agcttgcgcc gttcgccgcc actgagcgtg
    15721 taggcccgct gccgggcgac cttggtgagg ctcaggctcg ccagcagttc ctcggcgcgg
    15781 gccgcacgtt cggcgcgcga gagaggcagg gtctccagaa tcgccagcac gttctcctcc
    15841 accgtcagct tgcggaagat ggacgcctcc tgagcgaggt accccaggcc caggcgcgcg
    15901 cggcggtaca ccggcatccg cgtgatcggc tcgccgcgga acacgatctc gccggcgttg
    15961 ggccgtacca gccccaccac catatagaac gtcgtggtct tgcccgcacc gttcgggccg
    16021 agcaacccca cgatctcccc gcaactcgca tgcagcgaga cgccgttgac caccgtacgc
    16081 gaaccatact gcttgaccaa ctcgatggcc tgaatgtccg gtatgcgcgt ccctccgttt
    16141 tcgacaacgt cttccatgtc ccgtctcatt cccttagggg agccgcttcg tgtccatccg
    16201 cttaaacgtg ccgggcggaa gcaccacgcg cccgggcgag acttccatcc gctcgtcatc
    16261 aagccagaag gtgatccggt tacccgtgac ctcccccccc tcatctccct gccgcatcag
    16321 ccgcgcatta ccggtcatca caatctgacc gtcctttttg gtgtacacgg ccttgtcgca
    16381 actagccgac cggttcgcgt tcgtgatgct cacccgtccg accgccatga tctgctgcac
    16441 atcgttggta ccctccatga acaccagcag ccggtctgac cgcatgataa actgctcgtc
    16501 atccaccagc accttctcgt caaacaggat cacgccctct ttgttatcga actcgatccg
    16561 ttcagccgtg atcaccgcct cgcgttccgg tgcgccagtc gaatctcccg ccaacaggct
    16621 cgcggcgcgt gccgccctgt tcgtccgaac ctcaccccat gcggcccggc agatcaacag
    16681 gaccacgacc cagccgacaa taccaaacat ccttctcact tgagcctccc gaagttattg
    16741 cgtatgcgat gtgtccgaat ctcgcaatca cccagaatct taatacattg gcgctcggtt
    16801 gaaaagtaca tacccacacc ctgtagccgg tcgcccccct tttccacacg gacccgcccc
    16861 cggcaatacc cgtgccgggc cgtgcggtcg aaaaggcacc cctcggccgt caacaagcca
    16921 tccggtttcc cggtctcgct gaacatctcg acgcgcacct tctcggcaaa aatcatcccg
    16981 tccgggaaga tttgcgcctg atcggcgcgc aagagcgctt tggcccgccc gttcacgtga
    17041 tattccagcg ggatcgaaag cttcttcgcc ggcgaggtca tcgccgcctt gacctcgtcc
    17101 caacgcgccg caagcgggcg ccaaagatca tcgccgtgtc gcgcaaacca gcggtccgtc
    17161 tccggatcaa cgaaacgggc ggcctcgacc cgtcccaaca acagacaggc ccagaggaac
    17221 agcaaccgcc gcgccgcgcg ccgcaaagga ttaacgtacc agaccatgta tgcccttcaa
    17281 tgtgcgccac accgctttca aatcccggaa agcgatcgcg ttcgccttgt ccgagagcgc
    17341 ctcagccgga ttgacatggg tctccatgaa caccccgtcc acgcccgtgg ccaccgccgc
    17401 gcgcgccagg gccggagccc agcgcccgtc cccgccgctc atcttacccg cgccgccggg
    17461 acgctgcacg ctgtgcgtgg cgtcgaaaat caccggataa ccgaattcgc gcatgatcaa
    17521 cagactgcgc atgtccgcca ccaggttgtg atacccgaaa ctcgccccgc gttcggtgag
    17581 aaggatccgg cggttgccgg tgccggtgat cttgtcgatc acattcacca tatcctccgg
    17641 tgcgagaaac tgccctttct tgacgttcac caccctgccg gtctccgcgg ccgcgatcac
    17701 caggtcggtc tgccgacaga gaaaggccgg aatctgcaag atgtccgcca cctccgccac
    17761 tgccgccgcc tgccccggct cgtgaatgtc ggtcaggacc ggcacccgga accggtcgcg
    17821 cacctcccgc aaaatcgcca aaccctcgat cagccccggc ccccggtagg cgtcacccga
    17881 cgtgcggttc gccttgtcga acgaggcttt gaagaccagc ggcatctcca gttcgttcgc
    17941 gaggcggacc agtttcgccg ccaatccgat tgtggcttca gccgattcga tcacgcacgg
    18001 gccggcaatg attgccagtt ccggtgcccc gaacgtcaca catcccgcct taacccgact
    18061 taccctcccc atacatccgc tccttactgt tgcgcttgtc ctcgctcctt gagaatcgcc
    18121 tccaccgcca ccgcatccgc gggggtgtca atccccaaac cctgctcccc ggtcagaatc
    18181 accgcgatgc gcccccccag gtacagggca cgcaactgct ccagactctc gcattgctcc
    18241 aaggcgcacg gcggttccgt gaccagacgc cggagaaacg cgccgcgata ggcataaagc
    18301 cccacatgat gaagatagag tcccgaggcc aagtccggtt cgccatcgcg ccggcacggg
    18361 atgggcaacc gcgaaaaata gagtgcgccg ccatcatggg ccaccaccac cttcaccaca
    18421 ttccgcgact ccagttccgt gagagaccgg atcgggctcg cggccgtcgc catcgcccag
    18481 ccggggtcgt cccgcagacg gcgcaccagc gcgtcgatca gatccgggtc gatgagcggt
    18541 tcgtcgcctt ggatattcac cacaatgtcg tccttcgccg cacgcgccgc ctctgccacc
    18601 cggtccgtgc cggacggatg gtccggccgc gtcatcacca ccgcggcgaa gccctccgtc
    18661 gcgcgcgcga tccgctcgtc atccgtggcg accagcacct catccagcaa cgtcgcacgc
    18721 gttgccgcct ccaccaccca ttggatcagc ggtttaccgc aaagggggtg caggcttttt
    18781 cccggaaacc gggtcgatcc ccaccgcgaa gggattacac cgatcactcg catcgtttcg
    18841 actcctacca catcgtgaaa tgccccattc tccgtgttcc cagagagaga gtcaagccct
    18901 ccagtccgac aagtccgtat tttgtcgcgc gggaacggga cttttgccgg attgacaact
    18961 ccggcatgtg aaggcatgat aatcgccgaa ttgaagcagg agaatgtgga cggatgttcg
    19021 catctcagtg ccgaaaacgg aaggagagag ttatggcgcg cgtgtataat ttcagtgcgg
    19081 gtccggccat catgccgctg gaggtcttgc aggaggctca agccgaactg gtggattaca
    19141 agggttccgg catgtcgatt atggaaatgt cccatcgggg caaagagtac gatgccgtcc
    19201 acagcgaggc ggccgccaac atccgcgaac tgatggggct gaacgacgac tacgcggtcc
    19261 tgttcatgca gggaggcgcc agtttccagt tcgccatgct gccgatgaac tttctgggcg
    19321 aaggacgcac cgccgactac gtgaacgccg gttcctgggg cgccgcggca gtcaagcaag
    19381 cccgcctgat cggcaatgtg aaccttgccg ccgattgcca gaaggacatc cccacccgga
    19441 tgcctgacga gagcgagatg aagtggaccc ccggcgcggc ctacagtcac atcacaacga
    19501 acgagaccat ctccggggcg cagttcaaga ccataccgga aaccggatca ccgctcgtgg
    19561 cggacatgtc ttccgacatc ctctcccgcc cgcttgatta cagccgcttt gcgatgatct
    19621 atgccggagc ccagaaaaac ctcggtccct ccggcgtcac cttggtcgtg atccgcaagg
    19681 attttgcgga acagggcagc atcgcgctgc caaccctgct gcgctaccag acgcacatcg
    19741 agaacctttc gctgtacaac accccgccgt gtttcagtat ctacatgctc tgcctggtca
    19801 cccgctggat caaaaaacag ggactggaga acctttgcaa agtgaaccgc gacaaggcgc
    19861 agcagctcta cgatgtcatc gatgcctccg gcttctaccg cggcaccgcg aaacgcgaat
    19921 accggtcgga catgaatgtc accttccgcc tgcccaccga ggagctggag acgcttttca
    19981 tcaagcaggc agcggaagcc ggcatgaaac agctcaaagg ccaccgttcc gtcggcggaa
    20041 tccgcgcatc gatctacaac gccttcccgg ttgcgggcat cgaagcgctg gccgcattca
    20101 tgaaggattt cgaaaaacgc aacggctgag atccggacac cctactgacg ggcggcaagg
    20161 cgggggaatc cccgccttgc cggcaccttt gattttgaac atgcgttttc cataccccat
    20221 gctgatgctg ctctccgccg cgctcgtttc gcggtcggat gacgcgtatc cgaaaccggg
    20281 gtgggtcgaa aagccggatc cgctggccag cccgtacgcg gttccgggcg gcatgttgcg
    20341 cttcgccgcc ttccagcccc ccaaaagcct caacgcctac atcgattcca acacctacac
    20401 ccggcaggtt ttcggcctga tgtatgaaac gcttctggca accgatccgg tcaccaccga
    20461 attcaccccg tggctggcct gccgctggac cctttccgac gacaaacggt gttacacctt
    20521 cgagctggat cctgcggccc ggtggagtga cggagcaccg gtcaccgcag cggacgtgaa
    20581 gtggaccttt gaccagatca tggacccccg ccacgccacc ggagcatcca aagtcgcgct
    20641 tggcatcttc gattctccgg agcttttagg accgcgcacc gtgcggttcc gcgcgcgcga
    20701 aagccactgg cgtaatctgc tggccctcgg ctcgttcgag atcctgccgc gacacgccct
    20761 ggcggaacag gatttcaacc gcattgactt cgaccgtccc gtggtttccg ggccgtacgt
    20821 gctggacgct gtgcgggagc agatcgagat ccgcatgcgg cgccggcgcg actggtgggc
    20881 ggacgcgcgc cccgccaccc gccacacgat gaatttcgac acgctcattt tccgttacta
    20941 ctcggacacc ggaaacgcct ttgaagcctt caaaaagggc ttggtggacg tgtacgccat
    21001 ctatgcggcg cgtatctggg ccaacgagac gatcggtgag aagtttgaca agaactggat
    21061 tgtcaaatgc cgtgtgcgca accacaaccc catcggattc caggggttcg ccatgaacat
    21121 gcgtcgtccg cctttcgatg atctgcgcgt gcgccaggcg ctcgcccatc tggtggaccg
    21181 cgagaccatg aaccgcacca tgatgttcaa cgcctacttt ctgcaccgat cgtacttcga
    21241 ggacctgtac gacgacgccc acccctgcac caaccccgtc ttcgcgttcg acccgccccg
    21301 cgccaaggca ctgctgcgcg aggccgggta ccgccccaac ccgcagacag gcgttttgga
    21361 gaaggacgga cggccactgc gcttcacctt cctcacacgc gacggcggca ccgataaatt
    21421 cctcgccgtc tgtgccacga ccttccgcga ggccggcatc gagatgaaaa tcgaacgcaa
    21481 ggatttcgcc tcttggatgc gggacatgga tgagtaccgc ttcgacatca cctgggccgc
    21541 ctggggcggc tccctcttcc gcgacccgga gagcatgtgg cactccaaag aggcggatcg
    21601 gccttccggc aacaatctga ccggattcaa ggacccgcag gtcgacgccc tcatcgaaaa
    21661 acagaaaacc cttttcagca tcaccgagcg taatgccatc aaccgcgaga ttgacgcgcg
    21721 cgtggctgcg gcggtcccgt atgtcctgct ttggaacatc aactccacgc gcctgctgta
    21781 ttggaaccgg ttcggcgtgc cggacaccgt gctcgcgaaa ttcggcgacg aacggtcgtt
    21841 gctgggatac tggtgggatg acgcggactc cgccgccgag ttgaaagccg ccatgagcgc
    21901 gaaagatgtg ttgccccgtc ggccggagtg ggtcgacttt gacgcgatct tcccgtcgcc
    21961 atcttctccc tgacccgttt tattccgcaa agaaaccgaa aaaccaaccc aagaaagacc
    22021 cgtatgttcc ccctccagct tgatcgtccc ctcgtcgttt tcgatatcga agccaccgga
    22081 accagccccc gctccgaccg gatcatcgaa ttggccgcta tccgtctgga tcccgacggc
    22141 acggagaccg aagggtactg gctgctgaat cccggtgttc cgattccgat cgagaccacg
    22201 gcgattcacg ggatcaccga cgaacacgtc aaagaccagc cggaattcaa ggataaggcg
    22261 caggaaatcc tcgccttttt cggcgattcc gatctggcgg ggtttaatgc cggccgcttc
    22321 gacatcccca tgttgtctga agagttcgcc cgggtcggta tcccgttcga tgccctttcg
    22381 cgacgccttc tggacgcgca gcgcattttc cacaccaagg aaccgcgtga cctctcggca
    22441 gccctgacct tctactgcgg acgctcgcat gcggacgcgc acggtgccga agcagatgtg
    22501 cgcgccacgc tcgacgtctt gcgcggccag ttcgcccgct atgacgacct gccgcgcgag
    22561 atggaagcgc tcgacaagct cctgaatccg atcgacccct ttaatgcgga tcgctccgga
    22621 cgcctgcgtt ggatcgagga tgaggtggtc atcaatttcg gcaagaagaa aggggccaaa
    22681 ctgcgggatc tggccaaaga cgacccgggg tttctcaagt ggatcatcaa aaatgatttt
    22741 ccgcaggata cgcgcgctct gtgtgaaaac gcgctcaagg gtgtctttcc cacgccgcca
    22801 cgcccgaaat ccgcgccggt ccagtaacgt cctccggcac gcgtccgaag cggcgggctg
    22861 aatctccggc ttgcccgacc ggacgggaac gaatacacta aacccactac ttaaaaggag
    22921 acctcgaatg aaacgcatca tacagtgctc aaaatgcgaa gccaaactcg caatctttga
    22981 tttcgggaaa ccgatcaacc agaaatgccc caaatgcggg aacgcctttg tcgtggattc
    23041 ggaggccggc aaagcgaagg cgccagagtc ttccgcccct gccccagccc ccgcagccgc
    23101 tgacaaagcc ccgacccccg ctcccgcagc cacctcatcc gaacccccgg ccgcggctaa
    23161 acctaccgcc ccgactgagg cgcaggctcc cgccaaaacc gcgactccgg atgcgactcc
    23221 ggatgcggct ccggttaaac ccgaacctgc tgcagaaccg gccgccaaac cggttgacgc
    23281 cccggcggct tctcccgacg cgggccagaa ggagatcacg ctcaagaaac ccgctccgcg
    23341 tgccgccgcc aaacctgctc ccgcacccgc accggaacct gacatgcccg aacttccgaa
    23401 cagcggggtc tcattcctgc atgtggtcgt gatcatcgga ctgctggtcc tctcaatcat
    23461 cctgcaggtg atgaataaaa aagcggcgca gcgctcggcg ttcgaccttg ctgaacgcct
    23521 caaccggatc gaggcgaaac tgcgggcgcc acaacagcct taattggaaa cagggggcac
    23581 accggccgcg gacgaacatg gttcgctccg cgcgtcggtg aacccatgga caggggggga
    23641 caagtggaca cgaacaccct tgaagccaaa ctgaaagaga tgattgtcgg gcggctgttc
    23701 ctgaacgtca cggcagagga gatcgacacg aacgcctcgc tgattgacgc ctacggggtt
    23761 gactccgtct gtctgcttga actcgtggtc gggctggagg aagcgttcgg catcgtgatc
    23821 gaagacagcg actttgatgt ccgcaacttc atctcggtgg cagcgttgcg cgacttcgtg
    23881 caggcccgtc tgtaaaaaag agcagtcacc cgtacgtgaa caggtcggaa ggcgtgtact
    23941 cccgccctgt tgcccggttg agggccgcga ccacaatcct gcgcgtgttc ggcgtcagcc
    24001 aacgtccttt acaggcgcgc gcgaccattt tatgcgtcat cggcacctct gacgccgtga
    24061 ccagatcatg cggtgtgagt ccggcctctg acaagatccg ggccaagggc tgttcacccc
    24121 actcacgctg ttgcgttgta tccgacatgc aaatcttccc tcccccctgc cggttcagac
    24181 caccactccc gcaggttgcc attccggcag ttcggcatcc gccaacaggg gttgcgccgc
    24241 gtctttcgcc gagaggaccg gatcctgaat gggccagtcg acacgcacat ccggatcatc
    24301 ccagcggata ccccggtcat cgcccggatc gtagaactcc gaacatttgt acgcaatctc
    24361 ggcaccctcc gaaagcacgc agaacccgtg cgcaaaccct gccgggatgt agagctgatg
    24421 atgattctcc tccgtgagca ctgcagctga ccagcgcccg aaggtcggcg atccccggcg
    24481 gatgtcgacc gccacatcga agatctcccc ccgcagacac gaaaccagtt tcgcctgcgg
    24541 tttgtgaagc tgatagtgca acccccgcaa cacgccgcgc accgaacggg aacggttgtc
    24601 ctgcacgaac gtgtcacaga ccccgccctc ccgatattgc gccgcgtgat aggtttcgag
    24661 gaaaaatccg cgcgcatcgc ggaacacctg cgggacacac aggatcaccc cgtccaattc
    24721 ggtcggctcg aaacgtaccg gcatcacgac ctcccccgca ccgcgcggta gcgggcgatc
    24781 agcgtgttcg tcgacgcatc gtggcgcagc acgggggctt cgctcgcggt caattcgagc
    24841 aacactttgg aagccaggac cttgcccagt tccacccccc actggtcaaa cgagaaaatg
    24901 ttccaaatga taccctgcgt gaagaccttg tgctcataga gcgcgatcaa cgcgccgaac
    24961 gtctcgggcg tcagcgcgtc agccagcagc gtgttggtcg gatggttccc ctcaaaggtt
    25021 ttgtgcggca ccaacgtttc cggaaccccc tcggcgcggc aggcgtccgc ggttttgccg
    25081 aacgccaacg cctcggtctg cgcgaaaaaa ttcgccatca gtttgtcatg gtggtcgccc
    25141 aacggattgt gcgagcggca gaacccgatg aaatcgcagg ggatcagtcg cgtgccctga
    25201 tggatgagtt gatagaaggc gtgctgcccg ttggttccgg gctcacccca aacgaccggg
    25261 ccggtcgagt atccaacccg gcgtccctga cgatccaccg attttccgtt actctccatg
    25321 tcgagctgct gcagataggc cgccagccgg gcaaggtact ggtcatacgg caacacagcg
    25381 taactctccg cgccataccc gttcacgtag agcaccccca gcaaacccag aatcaccggc
    25441 atgttccgac tgaacggtgc cgtcgcgaaa tgccggtcca tcgcgtgaaa ccccttgagc
    25501 atcttccgga aaagacgggg gccgatcgcg atcatgagcg gtaacccgac cgctgagggt
    25561 agcgagtagc gtccccccac ccaatcccaa aacccgaacc tgttctccgg atcgatgccg
    25621 aactccgtca tccgtttcgc atcggtggaa accgcggcaa agtgtttggc caccgcggtt
    25681 tcactgccga gccgttccac cagccacgcc cgcgcgctca tcgcgttggt catggtctcc
    25741 tgcgtcgtga acgtcttcga cgccacgata aacaacgtct gctcagcctt gaccttccgc
    25801 agcgtctccg ccaaatgggt cgcgtccaca ttcgagacaa aatgcatctc cagcgaacgc
    25861 ttggcaaaag gtttcagcgc ctggcacgcc atggccggac ccaaatccga ccccccgatc
    25921 ccgaggttga cgacataccg gatgcgttta ccggtgtggc cgagccactt gcccttgcgc
    25981 acgcgttccg caaaacccga catggcgtcc aacaccgccg tcaccttggg catcacatcc
    26041 tgcccctgga catacaccgg acagtcagct ttgttgcgga gggccgtatg caacaccgca
    26101 cggttctcgg ttttgttgat cagctcaccc gagaacatgg catcgatctc tttccgcaac
    26161 ccactggctt cggccagctc caccagacga cgcatcagcg caggcgtaat gaggttttta
    26221 gaatagtcca gcgtccatcc ggcagcgcgc accgtgaacc gttccgcccg tttgggatcc
    26281 ttggcaaacc aatcccgcag atgcgtcgcg cgcgtcgccg ccacctcggt ttccaccgct
    26341 ttccatgctg attcattcat gaaataagtt tactatagcg gctccctctc cgcaatcccg
    26401 atagcgattg ggatgccaca ggccgggtcc ccattttttc gtcccgtggc tcacagaccg
    26461 gacagcgtga tcagatccgg cacctcttcc accggcgcgg tcccgcccgg caagagcacg
    26521 acccgcgttt caacccggcc gtcctgaggc gcagtcacct caaaggcgat catgcgcgcc
    26581 cccggattgg cgacatccca tggattcggc ggttcggcaa gatcgatcac ccgccatctc
    26641 acctccgccg gatgcaccac ccgcacggtc aggcttttgc cagagtgcgt cagcaccata
    26701 tcctgtccca ctgtctggca gcctgtggcc tgcgtcacca tggcccaacg gaccgcggcc
    26761 cctgcacgga cccccttgag gcgatcttga atcaccccgg tacgggaggg cagtaacgcg
    26821 cacgcgcgcc aaacccgttc cgcttgcccg gcgtagaccg ggctcagatc aagcaccgcg
    26881 tggggccgtt ccggattgtc gctgaagtgc gccacgctgc ctgatccgcc caccacatgc
    26941 ggttgcccgt cgatcgtgat aagattatga ctcgcgctgt tcagcctgaa caccttccac
    27001 cgatccgaat cctgttttga attccacagg ctcattccca acttctcgat cgaataatac
    27061 ctctgtgccc ccaagtccac cgcccaacgc accccatccg cctcgtagat gaacgacccc
    27121 gcatccatgt ggccgtggtt ttgtgaggca ggcccgcctt tcgtggccag atatccggca
    27181 ttgggatccg cccaggctgt acgcaacacc acaagcggca catgccctcc actcgaccag
    27241 tggagcggca tgcggctcgt aagctgatgt tcgtcgattt cccgtgccca gagcaagagc
    27301 agcgcgggca acgcgatctg ccgcaacccg gtcttgtcgg gattgttgag ccggagcgcc
    27361 tcccgcaggt cggcaacctc ccgcgtaagc caatcgaaac ggccgcttcg gcgcgccagc
    27421 caccacaacg cgagcagatg ggatcggcca tcaccgccgt ccgcatagtt gtagaaccgc
    27481 ccgctggggc ctgtcaccag attgatgtac tctccggtct gcgggaaccc cggcaaatca
    27541 tacagaccga aatccgtgcc gagagcctga gtcagcagat ccagcatgat cacgtaatac
    27601 gtcgtgccat agctccagta tcccggccct tccgggtaac ttcctttcgg ggcataaggc
    27661 cggatggagt agggaaccga cgtcaccgct ttatgaatga tcgacaacgc aagttcaggc
    27721 tcctgatcgt ggatcgcgag cgcccccgca accataccgc tccagcacac ctgagtccag
    27781 ttgttgttcc cctccgtcca gaaatacttc cgcgccaacg cttgtttcag gccttgatcc
    27841 cggatcgctc cggccacggt cgctcgcgtg tccggatcca gcgcgtcgta aagccagtcg
    27901 taccccagcg ccaagcccaa ggtcatggtg gccgtgtcca gaaaatgctt cgggttccaa
    27961 tcgctgaaag cggccgcggc caacatttcc tccttcgccc gctccacgta agcagccttc
    28021 ccggtgaaga ggtatgcggt tgccaacagc ccgacccgtt tctgcacctg actctggccc
    28081 tgcatccgga acccctcttg cttgcggacg gtcggtttct gcggcaagac cgcatcggct
    28141 tcccggatga tccaatcacg gatcacccct gccaccgaag gggttccgct ctcgctggcg
    28201 atcgatttaa acgcgtccgc ccgattgatg aacagccgcg ggtgcgtggc gggaagccgc
    28261 tccagacgcg cccgcacctc ttcccgtgtc accttcaacg gatcttgcgc ggccccccct
    28321 tgtgcccagc aggccccgca aaccagcacc cataccgcaa ccagacttct ccccattttt
    28381 tcctccttat gtatggtgga acaatacaaa aaaaagaccg ctccagacaa gagcgaactg
    28441 gaaaaacgtt ctataatagg ataccttcta tgaaaggagt tcatcatgcg cttttctctt
    28501 tcccatcgtc ccgccaccta tatcccgctt ctttgcatgt tgacgctctc gtccgcccgg
    28561 ctactgggcg cggacgagac catcctctgg ctggatacgc ttgacctcag ttcgatgacc
    28621 agcgcagggg aaccgcccca agccggtcgc acagtaggcg gcaacccgtt caaaatcgcg
    28681 cgcaagcgct ttgagcgcgg tgtcggcaca catgccgaaa gctggttgag tctggaggcg
    28741 cgcggcggcg cgctcgcgtt tcaggcatgg gtcggtgtcg atgacgagga gttgatccgc
    28801 ggcaaaggat cggtgatttt ccgtgtgtac gcggacggac ggcgggttgc ggacagtggc
    28861 atccggaggg cgcgggagcc cgcctttttg ctccgcgccg acctcaccgg ggcgcaggtg
    28921 gtgacgttgc acgtttccga cgccggggac ggtgatgccc gcgaccacgc tgattggtgc
    28981 gaagcgtttt tcacgctgaa gcagggagct tcgctgaccc ccctgccggc tccgccaacc
    29041 gaacagatgg gcatcctcac accggcgtcc cctcttgaac cgctccttcg cggaccgcgc
    29101 cgtttcggtg tccgacccgg cagcccgctc ctgttccgca ttcccgccac cggcgagaaa
    29161 cccatccggt tcgcggcacg cgacctgccg cccggcgtgt cgctggattc aacaaccggt
    29221 ctgctcacag gcgcggtcac gcgccccggc tcgtatgcga ttaccctggc cgcggaaaac
    29281 gcgcgcggcc gcacggagcg gatctggcat ctggaggtcg gcgagcgtct ggccctcacc
    29341 ccgcccctgg gatggaacgc atggaacgtt ttcgcgcata ccgtctcgga cgctaaaatc
    29401 cgtcaggcgg ccgaggccat ggtgagcagc ggactcgccg atcacggatg ggcgtacatt
    29461 aacatcgacg actattggca gacctgtccc ggagaacgag aggataccgc gttgatggga
    29521 cccggtcgtg atccctcggg gcgtattctg cccaaccggc gttttcccga catgcgcggc
    29581 ctggtgcagt acgtgcacgg actgggactc aagatcggac tgtactcctc gccggggccg
    29641 ctgacatgcg gcgggtgcgt cggcagttta gggcatgagg cacgcgacgc gcaaacgatt
    29701 gccgagtggg ggttcgacta tctgaaatac gattggtgca gttatgcgca gatcgcggcg
    29761 gatgacacgc tggcggaaca gatgcggcct ttcctgctca tgggtgccgc cctgcgcaaa
    29821 caaccgcgcg acatcctgtt tagtctctgc caatacggga tcggacacgt ctccgcttgg
    29881 ggggataagg caggcggtca ttgctggcgc accacccaag acatcacgga tacgtggtcg
    29941 agtatcagcc agatcgcgga agcccaagtc gggctggagc tttttgccgg gccgggaaac
    30001 tggaatgatc ccgatatgct ggtggtggga acggttggct ggggctcgcc ccgtccttca
    30061 cgcctgacac ccaacgagca atatacgcat gtctcactct ggtgcctgct cagcgcgccg
    30121 ctgctgatcg gatgcgatct gacgcgcctg gacgctttta ccctcaacct gctgaccaat
    30181 gatgaggttt tggaggtcaa tcaggatccg ctcggacgcc aagccgtccg tgtgcaacgc
    30241 actgacgcgc aagaggtctg ggccaaacct ctggaggacg gcagtctggc cgcgggaatc
    30301 atcaaccgcg gcatgttgac cgcccccgtg accctggatc ttactacgct cggcctcacc
    30361 ggcccgcagc aattacggga tctttggcgt caacgtgacc tcggcacctt tgccgacgcc
    30421 tccttcaaga ccaccctccc cggccacggc gtgctgctgt tgcggatcac accaatcaag
    30481 caatgaccgg catcggcggg gacgcctacg gtctcgtgtc tcccgtcttc tgtctgccgc
    30541 ttcgaagatc acgtgccgga aaccgccgcc tgctgttcgc gttgcaggcg gtcgagttcc
    30601 gtcgcatcgc gttcccactc ctcggaaaac acactaagct ggtcttgcac tcgttttagt
    30661 cgtttgttgg tggcggcgaa atcggtctcg ggtgtgggat tgaagagaca ggcggagagc
    30721 gtttccagtt ccgcctccaa atccgtgatg cgcgcctcgg tccgggcgac ccgttctttt
    30781 aacgtgcgga tggcggcctg atacttgctg cgagcctgcg cccgggcgcg ccgcgcgtcg
    30841 cgcgcgtgcg taacgggttc gtcggtcgtt gcctgttcga cgggaaccga ccgggaaacg
    30901 ttgtccgtgt tccgactctc ggtcgcgatc ttctcgcggt aataggtata cccgccggga
    30961 aaacggcgca caccggccga cgtgatttcg atgaccgatg tgaccgtgtt gcgcacaaat
    31021 tcgacgtcat ggctgacaag gcagaccgtg ccgttgtaag cgcgaagcgc cttttccagt
    31081 gtggcgcgcc cttccagatc caagtgcgtg gtcggctcgt caagcagcat aagattgggc
    31141 ggcgcaagaa agagacgcag gaacgcgagc cggatttttt cgccgccgct gagcacgcca
    31201 gccagctttc cgatatcgtc ggccgagaaa ccgaatgcgc ccaactgcgc ccggatcacc
    31261 ttttccggcg tatccggcgc gcaccgcttg gcgcactcgt acaccgagac gtccggcggc
    31321 accgtttcgg aaaaatcctg cgacaggtaa ccgggttgaa ccttgtggcc caagacacac
    31381 acgccagcgg tggggacacg cagaccggcg aggattcgca gcagcgtggt tttgcccatg
    31441 ccgttatagc cgacaatcgc gattttgtcg ccccgcgcaa tgctgaggtt cacatcccgc
    31501 aataccggac ggccgggatc atatgcgaaa tccacatgtt ccaaccgggc gacctccgca
    31561 ccggaatgcg gagcgggcgc gagtcgcaga tatcccgcag ccagactgcg tttgggcacc
    31621 ttgaccgcca ccgcctcgat tttttcgatc atcttcatcc ggctttgcgc ttgcgcggct
    31681 ttggaagcct gtgctttgaa gcggtcgaca aaacgttcga gttgttcgat ccgccgatcc
    31741 tggttggcct tggcggccag cagattagcg tgacgcgcct cacgctcgcg cagatagaaa
    31801 tcgagatcgc cgttgtatcg cgtggcggtg ttggcatcca cctccaccgt gaccgaagtc
    31861 agggtgcgca acagataccg gtcgtgcgag atcagcatca gggtgccgtc gtaccccgcc
    31921 agaaaacgct gcaaccactc caccgccggc aggtcgagat agttcgaagg ctcatccagc
    31981 aacagcaggt caggatggga agccagcacg cgcgaaagtt cggcgcgcat ctgccagccg
    32041 ccgctgaagt gcttgaacgg tttatcgaac tccgacgcat caaaacccag accgccgagc
    32101 gagaccttga cgcgggtctc catctcgtat ccgccgagat gctcgaactc ggtctggacg
    32161 tcacccagtt gacgcaacag gcggttgcgc gtctcctcgt cctccgctcc ggccaactgg
    32221 ttctgcagcg cgtggatctc ctcttccatc tcggtcaggc cgggaatccc gcgcaaggcg
    32281 tacgccagca acgtttcgtc ttcgtgatcc gccgcgagat gctgccggat atgcccgatg
    32341 cggggtgtgc cctcggtgat cacctcaccg gcatccgcgt cggcctcccc cagaatcagc
    32401 cggaagagtg tggatttgcc cgatccgttg gggccgacaa tcccgacccg ttcacccgta
    32461 ttcacgcgga aactcacctc ttccagcacg ctctgcgtac cgtaccgcac accgattttt
    32521 ttaaactcaa tcacagcaca tccatgatcc gcatacgggc gcagcccgcg aatgttccct
    32581 gcgcactctg attccgctca ggggacgacc aacacccgga acccgacctg atacaccggt
    32641 tgccaagccg cgtatcgcca gcgcgaggcg gaaccggcaa aagccatcgt gtcgttccac
    32701 gagccgccgc gtacgacctt tagccccgct tcgtcctcat cccccgaggg gtcgcacgga
    32761 tagggccgat acgcgctgcg cgtccactcg gcgacattgc cgtgcatgtc ataaagtccc
    32821 cagacgttcg gcttataccg gccaaccggc gcactgaacc gcgcgccgtc ttgataccct
    32881 gtctgcgccc ggccgtagtt ccagtcgcgg atgccttcat ccgccagatt ggcctgatgg
    32941 ttggcgagtg cgggtccgaa ggcaaagtcg gcatcgctgc cagcgcggca ggcccactcc
    33001 cactgcgctt cggtggggag agtacaagtt cgtccgctga cccgcgagag ccaggcgcag
    33061 aacgccatcg cctcgcgcca agagatgcgc accacggggt gacgcggcga tccgatgaac
    33121 gtgccgcgcg tcgtgcggtc tttgttacgc ccctcgatgt acccgctctc atgccgggca
    33181 ttgaaccggg catactgttc acaggtgacc tcccaaacgc cgagatagaa cggtttgtcg
    33241 atcgccaccg catgccgtgg cgcctcatcc gcgaacccgt cggcacgccc catcacgaac
    33301 gttccggcgg ggataagtgc gaaacgcatg ctgatccccc cgcccagatc cagagttagt
    33361 tccggttcac cggcggcccg ttgcgcggcc gtagcctgtc cggcattcat tggccagcca
    33421 tcgacctgcg gcgcggccgt cgttttcgcg accggagcgg acggcggctc aaagcgcgga
    33481 atcggcggaa gcggaacccc ctcctcatca cggtcttcca atccggcata ccgttttttg
    33541 tactcagcgc gcatgcgcac atgtttctcg ttcggcggca gattggactc acgccatgtg
    33601 ccgcagtacg ggacattgaa gtcgatccac gtgtagaggg cttcccactc ctcttgcgtc
    33661 aatgtaaccc cgtgatgccc tttcttaagc cgctgcacca actcgctggt gtcggcttcg
    33721 aactctgctg gagcgagcag gtggagatcc gcctccaagc cggcacggcg cacatacttc
    33781 tgcaagcgca tgtaggcggg actgaagcag aggggcggtt ccttgagatg cttattcgcc
    33841 gaaccgagcc accaccacca ctcgcatttg gggggaatca gactgggatc atgcggttgc
    33901 agccgtttgt cgcggaaatc ctccatcgtt gtttgccccc cgctcccgga cttcagcggc
    33961 ttgtcctgat gacaccccac gcaacgccgg ctgagaatcg gctggatctc gtgttcgaac
    34021 gaaaaaccgc gaaccggtcc ctgccacggg gtgatggcaa cgggtttgcc gcgcgcggcc
    34081 agattgggct gaaccggcgg cacggagttc tgcggctcgt gacatccgat gcaggcggcg
    34141 gtctcacccg gcatggcggt ataccaactg cgcatttgct gctgcgcttt gccctccgcg
    34201 tccagcggct gaacgaagat cggcgtgttg gcgggaaccc ggaaaaaagc cgaaccgtcc
    34261 tcatgcaccg gcacggtgcc aagaatgcgc ttcacatccc agcctccctc aaaagcgtgg
    34321 gcataggtga acccgttgcc aaagtatcga tagtggtggg tgccgacgcg cagcgctttg
    34381 atcgatccgc gtggataacc gcgcagacct tggccgctat agatgtcgga aagatagatc
    34441 actgcgtctt tccgggaggg attgcattgc gacgggatca ccggcggctg cggccgggcg
    34501 cgcagcggca ccggcgacat atagttgacg aactgcccgt tcggcagagt ttcaaaatgt
    34561 ttgatgggcg tgacattatc gaaaatatcg atcaggcaga gatcccagcc cgagaaggca
    34621 ttgcgttggc ggcacaccag aaagtagcgc ccggcaccca gcgccgcagg cccttcggcc
    34681 agcggatacg gggcagcgta acgcggccag acatcggaga cgagctggtc cttgatcacc
    34741 ggctccacgg tttgtccgta tcccggcaag cgttgcacca cgccagatgc ctcgtgccgc
    34801 ccctgacgcg gatccagcag aatcacctcc cctgcccggc tgaccccgtg atgcccggag
    34861 acgatgcaga ccacgcggtg cggatcaccg gggatcggac gcggccagta catggcgttg
    34921 ggccagtacg aaccgcttcc gtagtactcc atctgacccg aaccgtcggg attcatccgc
    34981 atgaggaaac gtgagaaata gtgcggtatg tcggcatact cccagcgcgt atagagcacg
    35041 cggccgtcat gcatcaccgt ggggttccag ttgtgatcct ggtcaaaggc gatccggcgt
    35101 tcgttacggc cgtcggcatc catccggtgc aggttgccga cattcgcctc gccggtgcag
    35161 ggcacagcct gataacaggc gttacagacg gccagcaagc cgccgtccgg caaatagcat
    35221 ccgtcataat gccacacatc gggcggcgcg tccgtgatcc ggcgcggcgg gtcgtcgggt
    35281 gcctgcaagt caagttccca gaggatcttg cggtcggaga acaagacacg ccgaccgtcc
    35341 cagtgcagat cgaaatcgga aaccgttccc ttgtgcacac gcgtcacgct accgctaggc
    35401 gccaccggcg agagaatggc gagttcatca ccgatctgat tggctccgcc ccaattgctg
    35461 ttgaaagaga ccgaaccgcg ggccagcagc acccggttaa acgcgagcag cggggtgtcg
    35521 agcagcacgg tctgccgcaa ggcgtcgagc gcgacagact gtcgggcggc acgttcagga
    35581 agatccggcc cactatgcgg gaaggcgcgt agttcctccg cgatttcccg ggatcgcgca
    35641 tcgatttgcc gccgtgccgc atgaccctgc ggataggcat ctttgaacat gcggatatgg
    35701 tcttcgaccg ccagacgtat ggagatcacg gttgccgcgc tggtcatcag ttcacgcagg
    35761 gcacaacttt tcagaaacgc cgcacgggga tcagaccgac gccacgccgc ctccggtttc
    35821 cgcgcctgcc cgacccacgt ttccagcatc tcccggtacg gggtcagcgc ctgcgccggg
    35881 aggccctcca tgcgcgccag ccgacccgcg atctcagcca ggacatcgtt acaggcggtc
    35941 gcaaagcggt ttgaagcgaa ctccgcctca catccgggga cccaggcata ccgataccgg
    36001 ttgtactcct gaggcaccca gaccgacgcc aacgcctccc actcggccat tcgaatctga
    36061 tgcggcgcgg cgtcaacctc ggccaatgcc cggcgcatca gagccagacg ccgctgggaa
    36121 cgctgccaac tgtggctggc cggagattgt gtgttacggc ggaacacgcg gaaataaaag
    36181 cggcgatagc cgtcgcgatc cggtctgatg accatcatga actggttcgg ccccggacgg
    36241 atggtcagcg catacccgtc atccaacacc ggccatgtcc cctgttcatc ccgccggttg
    36301 cgtcccggat gccactgcga atacccgtcg ctcagggaca catcgagcat gacgtccttc
    36361 agatcgcacc gctcagccag cgtcaccgga cggatcgcga tgaagcgatc cccgggtccc
    36421 gcattgcgga aggccgtcag atcacagacc gcatggtcct tgaactgcgg acagggaatc
    36481 caggcgtgcc gtccgtgccg atcctccaac ggaacagtga ggtcgatcag gccgtactca
    36541 ttgataatct tgtccgtcag cggagagtcg ggttttagcg gccccaacac atgccaaacc
    36601 ccgagttcga gcccttcggg cgcggccgcg cgcgcatcgt gtgccgcctc ccaattacgg
    36661 agcgcaccca gattgtgcag catggtttcg ctcaaggtcg cgcgccgcgc aaactccggc
    36721 ggattgggca acggttcgag ttccgcaacc gtagtcagga ccaccccggc aacggcaatc
    36781 aggttagcgg tccgccaaca tgcggaaaag ttctttgtaa aacgagtcat gcgttcacct
    36841 gatccattcg gcgctcacgg tttcacgtaa gtcagattgg tgcagacctg ggtcacttcg
    36901 tgccggaccg gccagaccgc gcgttcagga ctagcgtaaa gccccatttt ctcgatgggc
    36961 aggggacgga accccagctt gaaagcggga gacgccgact gcaggcggta atcccctttg
    37021 gcggcatcga taaaaagagg atccgcaccc gcgagattgt tttcgccgac cagccaggaa
    37081 tgaaccgtgc gaggatattt tttataatgc gccaaccaca cggacggtcc gccccaaatc
    37141 acgttgcgct cgacccgtgt gcgcgtttcc ggcggacgga gttttttatc gcgcaccgca
    37201 ttacggaagg ccaacagcca aggataacgt tctttccaca gcggatcctc caccggcgtg
    37261 gcgaagaggc gttcgggcat ttgcgcatgc acccccttcg gtggcgtgac cgactccggc
    37321 gtctcatagg tctgccatcc ggaaatcgcg ggatggcaat caacaaacac gttgttgtcg
    37381 atcacgttac ccaccgccga gagcgagatg ccctcggcta cgcggacaat cagattctgc
    37441 gtcatggtga tgccagccgc gccgtcatcc agcatgatcg cgcgattgcg ctgcggtcgc
    37501 gggttataga tatcgtggat atagttgccg tggatcacat tcccgaccag cgtccagtcg
    37561 cggccggcgt aaaacgcgcc catctcaccc gaggagagac agatgctatg gatctcgttg
    37621 tactccatca cctgttcccg cccgctgaac agcaccgcca catgcggtcc gttatgaatc
    37681 aggttgtgcg aaacccggat tccgcaaccg tagagctgga ccgccgcacc ataggtcatg
    37741 ctgaagcgcg cgtaatcatg gatatggttg ttgtcaacga catgtccgca gcgctcgagg
    37801 ctccgctcat tcccaccgga gacctggatg cctccgtcgc ccagacgcgt catctcgcac
    37861 cccgcgatcc gatgcgtttt tccgccgcgg atcgtcaccg cccggtgccc gatgttccgc
    37921 attgtgcaac cgatcacgtt caccgactcg ccctgactga tcaggatcgc ctcgccgcgg
    37981 caattctcga acgtcagccc ccgaaactgc atatgggcga cgtcgtccaa ctggatcagc
    38041 cgcgtcccta ccgacacttc ggcacgggtg ttcgcagatt tcccgggggg ccaaaagtag
    38101 agtttgccgt ccagccggtc cacatagtac tcgcccggct tatccaattc gcacaacagg
    38161 ttataaccga accagtgccc acctttgcgg aatccgtagt ggctacccgg gaccttctgc
    38221 gtgatcgtct tcgttgccgg gtcgatccgc tcaaacgcga tcgcgcaagc cgcccagtcg
    38281 tgcgcccaat acccctgtcc gcgcggatcg atctcgtcaa tccagcggga aggattctcc
    38341 tccgcatacg tgaaggtgtt tccctgtttg ccgtccggcg tatccgcgat ggtcacgaat
    38401 ccctcgttgg gccagcgcgc caaccgcatc acctcgtcac cgtagatgag ttggatgccg
    38461 gacacaggtc cggcagagga tccgaccggt tccacgccgt actcgtaaac tccctgcgct
    38521 ggcaacgaga tcacctggac cttgccgcgc gcggcctccg gcaaccgccg cagcagattg
    38581 gtgtcggtca acgtgttcat cttccacaac gccacaccgc ccgtgatccg cacctgcccg
    38641 ttcggggcgg cctcgaaaac gaccggcgct tccggcgaac ccgaatcctc tttcgtgaaa
    38701 acaaccgggg tccgttgctc ataacggccg atctgaaaac gtaccgtgac cgcctgcttc
    38761 ggcgtcaacg tgccggcatc gcgcaaccgc cgcaacgcat cccgggctcc gcccagagag
    38821 gcgagaggcc cgtcggtctt cgccgcgttc ggcgcggcta acgtgccgct ccacgtgtct
    38881 ttcccctgcg gcgaaacgtg cagcgtcacg ttagccgcaa gcgcaccgca cacacccagt
    38941 aaaaaacaaa gtccagccac cccgcctcga cacatcggtt ccccccttta caatgaacga
    39001 ttcagttaag cagacgcttc atcgtttgac aagcgttatg ggcggaaaca cgcggacgca
    39061 tcacggtttc agattttcgt atcatgaaac acatgaattt tcatgaaaac agggacaggc
    39121 aaagaatcgg gagcgcggcc gtcctcgccc gctggcaggg cggtttcccc gcaaaccgcc
    39181 gccggtccct gccgagcgga cgcctccctg atgcgtccct agttatccgt ggcaggcctc
    39241 atgacacaac ggaaatccgc ctcggccgcc agttcttctc ccacatacgc cttgccatgc
    39301 tggacggcca tgcgcttatt cacgcgccga ttttccacct ccatccgcag cgtatcgccc
    39361 ggacggacct gccgcctgaa cttggcgttg tccacgccga ccagcagaaa cacgccgttc
    39421 tggatcagac cggaggcaac cagccccgcg cccccgcact gcgccatcgt ttcgatcagg
    39481 ataacgcccg gcacaacggg gtatcccggg aaatgaccct gaaaaaacca catgtcctcg
    39541 ccataggtca tctctccgat atagttctgc tgatcgaaat acaacaagcg gtccacgaac
    39601 agaaacggtt tccggtgcgg aagcagttct tcaattgatc ccggatattc cttgccgctc
    39661 gccacgagtt gctgccatgt tattttctct tcgttactca tgctcatccc cttcgccacg
    39721 gatccggaga ggatacggag tttacctccc cttccgcgtt tgccgtttac acggacttca
    39781 ataccagaac gccattgtgt ccaccgaacc cgagcgaggt ggaaagggcc gcgcgaatcg
    39841 gtccgttcac cccctgattg ggcaccacat ccagatcgca cgccggatcg ggtgtcgtgt
    39901 agttgatggt cggcggatag aatccgtccc gaatggccag cgtgcagatc agcgcctcaa
    39961 tcgcgcctgc ggcacccagc atatgaccgg tcatgctctt ggtcgaggac atcttcagtt
    40021 tacgcgcatg gtcaccgaac accgtcttca ctgccttggt ctccatggga tcgttcaacg
    40081 gggtggaagt gccgtgggcg ttgacgtaat cgatctcatc cggcgcgagt ccggcatctt
    40141 tcagggcaag acggatcgcg cggatggcac ccgccgcctc cggatcgggc gcggtcagat
    40201 gataggcgtc tccggacgcg ccgtagccgg cgagttccgc gtagatccgc gctccgcggg
    40261 cgcgggcatg cgcctcgccc tccagaatca ggatcgcaga accctccccc atgataaacc
    40321 cgtcgcgccc ggcatcaaag gggcggcagg ccttttcagg cgtgtcgttg tacttggtgc
    40381 tgagcgcctt cattgaacag aacccgccca cacaatattc ggtgaccgtc gcctctgtcc
    40441 cgcccgcgat catgacatcc gcccgtccgg cgcgaatcat gtccatcgcg accccgaggg
    40501 cgtccgtgcc cgacgcgcag gccgtgacca gcgtgtgcac ggcggcctgg gccttgagcg
    40561 cgatcgagat gtttccggca gcctcattcg cgatcagttt gggaatcagc atcggcgaga
    40621 gccggcccgg cccgcggttg aacagcacct gatatgcctc gtcatccacc tctttgccac
    40681 cgatgccgtt cccgacaaac acccccacgc gctccggatc cgcgcccccg tccgccaatc
    40741 cggcatcctg ccaggcctgc ctggccgctg ccagcgcgta ctgcgtgaag agcgccatgc
    40801 ggcgcgcctc cttggggtca acgtactggg tgatatcgaa ctgcttcact tcacccgaaa
    40861 tcttggaatc cacacgcgag ggatcgaacc gggtggtgcg tgcgatccca gacaccccat
    40921 tccggatatt gtcccacatc tcccccaccg tgttgccgac aggcgaaatc acgcccaaac
    40981 cggtgatgac aaccttggtt ttcccgctca taagcactgg tctccttttt ttcccacttg
    41041 gggtacgcta agcactctcc ggatgcggac gctgcggcgg gacgccaccc gccacgtcgt
    41101 gccactggag caaaatcccg ccgtaggtca ggccgccgcc gaacccgatc agcacaacca
    41161 accctccgtc cgtaagctta ccggaacgga ccgcgtcatc cagcgcgacc ggcacggagg
    41221 ccgcagaggt gttaccggtg gtcgcgacgt tcatgtggaa tgtttccaat ggcaccttca
    41281 tacggcgcgc caccgcctcg acgatccgcg cattcgcttg atgcgggatc acgcacgcca
    41341 gatcggcgaa cgtatggccc gaccgttcga gcagcccgca gaggatctcg tcgaacacct
    41401 tgaccgcgaa attgaaaaca ggccgtcctt tcatgaacag cgtattgtgc atccatggac
    41461 cggtcgcgag ttgccgcgcc cccccttcac ggtacaacag gtccgcaccc gaaccgtcgg
    41521 cgcgcaggat ggcatcgcgg attccgccgg actgcccgct ggtctgcaga accaccgcgc
    41581 cggccgcatc cccgaaaaga atacaggaat tacggtccgt ccaatccaca atgcgcgaca
    41641 tcatctccgc tccgatcacc agcgtaggcc ggggatccac ctgcatcaga ccgtaaacca
    41701 tccacagggc atagatgaac ccggtacagg ccgcctgcag atcaaacgcc gcggcctgcg
    41761 tcgcacccaa tgcgtgctgc accagacaag cggtcgaggg gaaagcctga taatcagatg
    41821 tggacgtggc gaccacgatc atgcccaact cttcagcacg cacacccgcg gcctccaacg
    41881 ccgcgcggcc ggcctggatc gccatggatg aagtggtttc gtcatccgcg ccgagatgcc
    41941 gactccggat cccggtgtgc gaaaaaatcc attcatctgt ggtgtccagt ttctccgcca
    42001 actccgcgtt ggtgaccacg cgcgtcggca gatacgaacc tgtaccggtg atatggacaa
    42061 acatgccaat cctttcgtgc tgaaaacaac gcttgatcat gacgtttttt gttgttttgt
    42121 caacctgcaa tgcgtcgcat ctgaaatttt gtctcaggaa aaagaaaccg attcgaaacg
    42181 ggcggttgac agggaaaacc cggcctgata tagtgaccgc tcgttatggg acagcaactt
    42241 agaaaacgtt tgaagcgcca gcgccgggaa gcccgcaaca agcggatcaa ccagaagctg
    42301 cgcgccgcga tgaaaaagta aatttttcat aagggtgcga tggccgagtg gttaggcaga
    42361 ggtctgcaaa acctcgtaca gcggttcaat tccgcttcgc acctccatgt ttccactgat
    42421 catcacagat tgtgacggtc agtttttttt gtggctgccc ttcgtgaaat gcaggcgcgc
    42481 aaaaaaaccg cgccccgcgc ggttttggcc gcacggggcg tggcacgtga aaagaaaagg
    42541 ccgagccgct aacggcgaac cgccagcggc tctgcgtctc ttacatgtcc atgccgc
//
LOCUS       DAOSLE010000007        41752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1091_length_41752_cov_6.996427, whole genome shotgun sequence.
ACCESSION   DAOSLE010000007 DAOSLE010000000
VERSION     DAOSLE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 41752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41752
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1091_length_41752_cov_6.996427"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PK576_01050"
     CDS             <1..643
                     /locus_tag="PK576_01050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002762309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOE60209.1"
                     /translation="RKLTITVERDRSVVVRAPVGVSDEKIREIVEAKRFWIFEKLHHV
                     QKYTPPHPPGKEMVSGESVLYLGRSCRIEVVDTDATEILFDHRFVIPHSHASRRKEVL
                     REWYIERAKATILPRVRRFADKLGVDYKHAKIVDSRYRWGSCTVKDNVNFNWRLIKAP
                     MFVIDYVIVHELAHLMEPNHTPRFWNIVSAQSPKMEKARAWLRDNGQLLEEDI"
     gene            complement(664..1428)
                     /locus_tag="PK576_01055"
     CDS             complement(664..1428)
                     /locus_tag="PK576_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE60210.1"
                     /translation="MAQAPTLSDIAYRAITRKLMASELLAGQKLSEQKIAAECGISRT
                     PVREAVRRLIEEGVLYQIPCSGTYVARLDRRQLIDAYEVRMALECFALDRAVRNLTRE
                     QRAECRQTCDVMRKVAEGLRKRNKTVLDGKPLVTFLKADLSFHLLLLKAAGNALAIKI
                     VTHAYQRNLFFGHYSHRRDLNHVAWAWHHHAEIERAIRRGDADRATRWMRTHIARSRE
                     DALIAFDQAQISLIHANHPPRDPVHEALDRFASQLV"
     gene            complement(1572..3806)
                     /locus_tag="PK576_01060"
     CDS             complement(1572..3806)
                     /locus_tag="PK576_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60211.1"
                     /translation="MSIESLKNYVFTSKYSRYIPEKMRRETFEEAVDRVIAMHRSHFA
                     AKGIELEDLLSLCEQAMKKRMVLGSQRAMQFGGPPILRKNARIYNCTTSYCDRPRFFQ
                     EALWLLLCGCGVGFSVQPHHIDKLPGIRKPAAGHATFIIPDTIEGWADALGVLLSSYF
                     TDAQPFPEYAGQIVQFDYTEIRPKGRPISSGMGRAPGPEPLRRSLEMIRTLVDRRIEE
                     GHGRLRSVDAYDILMHASDAVLSGGVRRSATICLFSPDDELMATAKTGNWFNENPQRA
                     RSNNSAVLLRNQTTREQFNRLMKSVKEFGEPGFVWTDHLDMTFNPCGEIGLYPQIDGV
                     TGFAFCNLCEINMGLVDNADKFREACRAAAILGTLQADYTDFEYLGEVSTRIAKREAL
                     LGISMTGMMENPDVSFDPQLQRDMAKVILEVNAEVAARIGINPAARTTCVKPAGTTSS
                     ILGTSSGIHPHHAKRYFRRAQANEDELLVDFFGAQNPKAVEKSVWNPNGTDVVLTFCV
                     EASPHALTKRDVDAVELLNKVKLTKENWIDAGKRPEQCVQPWLSHNVSNTISVKDSEW
                     ESVADFIYENRHVFAGVSLLPDGGDLDYPQAPFCEVLTADEIVEIYGVGALLASGLIV
                     DGLHAFDDNLWAACDCVLGRGENLEEPTPKTYPKRVYEAIEGVREAKQDWVRRARKFA
                     RNYFGNDVLKMTRCLKRVNACKHWEDLTRTYAPVDYTQLIERQDNTTVTQTVACTGGK
                     CEIL"
     gene            4017..5069
                     /gene="gluQRS"
                     /locus_tag="PK576_01065"
     CDS             4017..5069
                     /gene="gluQRS"
                     /locus_tag="PK576_01065"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOE60212.1"
                     /translation="MGNDQREVREEALQSDAARGDETGGSAPVRGRLAPSPTGALHLG
                     NARTFLIAWLRVRSRAGSVILRMEDLDHPKIKRGAARETLDDLRWLGFDWDEGPDCGG
                     PFAPYTQSERRAYYARVLQRLIARGRVYPCICSRRDVEAGQSAPHAEDGLFYPGTCRG
                     RFADYPDACRVLPTGRLPAWRFRVAAGERVAFDDKFHGRVEQDVAAASGDFVLARDPD
                     GAGYMLAVVADDAAMGITEVVRGDDLLPATPRQILLYRELGFPPPLFVHVPLVVSEEG
                     RRLAKRHGDTRIRAFREAGVPPEKIIGLLAWWCGWAEWGEMLSLNEVLPRFDMRRLPR
                     EPVVLTSAIRRELGIR"
     gene            complement(5145..6317)
                     /gene="aroC"
                     /locus_tag="PK576_01070"
     CDS             complement(5145..6317)
                     /gene="aroC"
                     /locus_tag="PK576_01070"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOE60213.1"
                     /translation="MALRYLTAGESHGPALTAILEGMPAGLPVTEGQIALDLARRQCG
                     KGSGERMAIEKDAAQILSGVMDGVTTGAPIALLIPNRDHANWKGREIPAYTTPRPGHA
                     DLAAAVKYGYDDIRPALERASARETAMRVAVGALCRALLDNFGITVGGYVSAIGEHTA
                     RLDGLAFAERVSRARASAVQCPDPVADAAMSDAIAKAHDAGETLGGIMEVVADGIPVG
                     LGSHVNADRRLDARLIQALGSVPAIKGVAFGDAFASARQTGTLTNDAIRPDAEGNLLR
                     GSNRCGGIEGGISNGAPIWLRAAMKPIPTTRKGQETVDLATGQTSLTCYERSDVCPVP
                     RAVVVLEAVVAIVLADALIEKLGGDSLSEMRPRFDALRRATLREARMSGNPRLFWP"
     gene            6469..7545
                     /locus_tag="PK576_01075"
     CDS             6469..7545
                     /locus_tag="PK576_01075"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOE60214.1"
                     /translation="MAKTYKIAVLPGDGTGPEVIAEGCKVLDAAAQKFGFKLDKAFYD
                     LGGMRYLAIGETLTDATLDELRQFQAIYLGAIGHPDIKPGILEQGVLLKLRFALDQYI
                     NLRPVKLYPGVACPIKDKGPAEIDYCVIRENSGGLYTGIGGLSRPGTRDEVAVQQMIY
                     TYAQVERCLRYAFEYARHNGKKARGKGPENKLALIGKSNVLTHMYALWNRVFDEVGSE
                     YPDVKREYYHVDATCLYMVACPEMFDVLVTENMFGDIITDLAAITQGGLGVAAGGNIN
                     PQGVSMFEPIGGTAPMWTGKNAINPIAAIGAGAMMLTALGETEAGRAIEDAIAKTTPK
                     MKSQMANEMGFTTTQVGDLVAEAL"
     gene            complement(7553..8152)
                     /gene="ruvA"
                     /locus_tag="PK576_01080"
     CDS             complement(7553..8152)
                     /gene="ruvA"
                     /locus_tag="PK576_01080"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOE60215.1"
                     /translation="MIAFLQGTVVEKTPSSVVLDVHGVGYEVFISLNTYDRLPGSGAP
                     CRLLTHDYVREDARTLFGFMQAEEKAMFERLITVSGIGPKLALSALSGLTVADLSLAI
                     AEGNVKRLSSIHGIGKKTAERIIVELRDKIDPLAALAARSGEARTSEQTIMLRDAILA
                     LGSLGFPQEQARKMVQSALDAEPGIRETEALLRKALSTK"
     gene            complement(8149..9435)
                     /locus_tag="PK576_01085"
     CDS             complement(8149..9435)
                     /locus_tag="PK576_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE60216.1"
                     /translation="MNTLALRLYGENDLRLERFPLPEITDDEILADIVTNSICMSDHK
                     AAIQGARHKRVPDDVADHPIILGHEFCGKFLKVGKNWQHKFSVGQKYGVQPQLNIPGH
                     EHRAPGYSFPYTGGNATRIIIPREVLELDCLLIYSGDAYYKSSIAEPVSCIVGAYRCS
                     YHFKAGEYVHQMGIKNGGSMVILAGAGPMGLSAIDVALHGPEHRPGRLVITDIDQARL
                     DRAAQLFPVEHAKTCGVELHYINTAGQDDPVAQIRSVNANQGFDDVMVFAPVPELIEQ
                     GSALLAYLGCLNFFAGPARSDFKAAINFYDVHYMGHHVVGSSGGNTQDLQDAMDYAAQ
                     GLITPSVMITHVGGIDSVAETTMKLPDIPGGKKLVYTHVSMPMTAITDFAARGRTEPF
                     FAALDEICGRNNGLWCSEAENYVLTHAPRLEEDARV"
     gene            complement(9919..10986)
                     /locus_tag="PK576_01090"
     CDS             complement(9919..10986)
                     /locus_tag="PK576_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60217.1"
                     /translation="MIDAKTIKKVNTVNVNCKMKHTLSLLSLRRYAIPALLIGLLLFT
                     VSGWLTGLTGRLRWRLDQGNQIYLDASIRDAARLMIPVGTAKAAADAIEGSTVNIEAG
                     AVFAKAGMSIEAGDTLQPLLDYIDLAWRLLMISLVYLVTAKCVLAGTDALSAPFLVLS
                     FSALLAGHLFAMIPGIPPVWRQAFRRFGALFLMAALLFLLILPLTVAGTAYLSRHTTD
                     PMREEVRTAFEQIGSAFSMEQFHAADELKDKALILKEKLASLGEYARVSIKEVAMAVC
                     KLVAIKILNGLVFPLASFAFLVWLVRGCLWPAFGLSDRPLSPEDFRRLATWLDRGKPA
                     THAEPPTVLPTEPAESSPGER"
     gene            complement(10979..12358)
                     /locus_tag="PK576_01095"
     CDS             complement(10979..12358)
                     /locus_tag="PK576_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE60218.1"
                     /translation="MKKSLFALSLFAGLTTLAATITDITVSPRWPWQDKVDIDYTLDS
                     DVYGGFPVTVALYDGETPLTVAAASLPGNGEVRLPGRRHQVFDFALSGLESRPKALRT
                     VITTSANPVRFMIIDLTKRPGDANQREYIYAGDARLESYVDYPIEYRVSDDEAEGMAV
                     THIDYTDVWFGVTNDNQYARSKMVFRYVPPQAGVKTGDGNVETVFNRVSLTKAYWIAV
                     FPMTQGQYSWLTWDVANRRHRDPNWGMATINETEQQYPMPGGMRDYVGATYTDDPYND
                     YPAGMRGPVGDPVNPVGWPTYGHDVCPTNLLALYRQKTGLRLDYPTEAQWEVACRAGT
                     TGYYYDKESTAQNTNLLQRIANKIDNNVAVGRYLPNTWGLYDMLGYRGEVMLDRYTSD
                     RISGVDPVGDRDPAKSNYTYARVVRGTSSHAEPHAQATQVGSRIADAAAEWFSSLLTT
                     MRWTVELDD"
     gene            complement(12378..13727)
                     /locus_tag="PK576_01100"
     CDS             complement(12378..13727)
                     /locus_tag="PK576_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60219.1"
                     /translation="MKHLVPVLALSAVSAASAVTLSIDSVKQRWPFSPKIDITFTVGE
                     TGGDFYRLKDFAIYDGNHQVALNGTAAFDNLKPVYSAGTYTLTFDPSKTALTNIVAVQ
                     HFRIGFDISATPTRYLIVNLDKQVDEDGLVEYIDQGDPRLETFVQTVDYLDNGTPTQT
                     TIQYSDAFLSLTNMTVSSGGKTASPYAINKMAFRLVKPGDYRVGKASVSSTYNSVTFT
                     KPYFIAVGLLSRGQYGYIRYGSPWQTDTGTDGNAENQSKSKEIYLDDLRGPHNDPVYP
                     VDWITYGHSVCPTSFIGQARIKYGLAFDQPTEAQWEVAARAGTTGFYYVDVNLTAENT
                     DLLLKLGHEGGTNFNPFRKLANGWGLFDLLGWGQGVLDNAKSSDAGQVNNWVWPSGTD
                     PVGAYTTWNGFCICKGGGHHAGLLEACGSRPTGLSRTKYVNWYDGGYNRVRLVINVD"
     gene            complement(13742..14410)
                     /locus_tag="PK576_01105"
     CDS             complement(13742..14410)
                     /locus_tag="PK576_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60220.1"
                     /translation="MIKSRSCVRSVLVSLVIGFVLAANAALSPTMKIIAPLAGSNLDW
                     GQLHATTVQLNWSWPNGATSAELVIVANGRENAVFSRTFPDTAVRSCSWDMGVPVEDT
                     VYDVTLTFNNNVVQKAQLYANLGSFGGVELKGWNDAGEAKVTKGCVLPYRAAWAEGLA
                     GAATLSIDAEQIALDNSDGYSYLRFSQPGVRQAELDFETEPSSPAFSAYLKVVCNGFV
                     VLFF"
     gene            14675..15904
                     /locus_tag="PK576_01110"
     CDS             14675..15904
                     /locus_tag="PK576_01110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOE60221.1"
                     /translation="MKKWMVMFIAVAAMISGCSTVCNGPKNSGTTFAKDALAPFVEKG
                     ELPGAINVFYKDGLQEVACVGYADVAAKRPITMDDVFMQCSQTKGFCGVTIAILVEEG
                     KITLDDPVSKYLPEFKTLWVHESTTNGVKRLVQAKNPLTIRMVMNHTGGFPFEICAKQ
                     GNIKGGGWSGGAPLRQTAAIAAACPLLFEPGTKVQYSNTGIDIGAAIIEVVTGTRWED
                     FLKERVFDPLGMASSSFKPTDKQLKTQVEMYDCVKDAPAKHRLQNEMQQRPYNGDHVF
                     ASAGAGLWTTASDQLKFYKMLMNLGVGENGVRILKEETVKEILAKSTRPKGMGGYSLG
                     LTAPEEDKEDSWFGHGGAWGTNCMVNWHRRELKLWAVQLCGSPRPWDKARTAAADKFF
                     QYAIDNSSADAYTGRVQ"
     gene            16648..19485
                     /locus_tag="PK576_01115"
     CDS             16648..19485
                     /locus_tag="PK576_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60222.1"
                     /translation="MCKRLCRMVVALSMITVVRADQHKWKSAVSGNWSDLSKWSIASA
                     LPEVTDTIQYGAVGTFTITNDTGGDITLVKIDQSAVPGATKFCVMTLDMQGRTLTLTV
                     SDNSSIFSSGYYQYKNTRDLDPPTCTYELRNGTFDFTAGRLFLGNNGMEADGGIILSG
                     GATLHAYTYHWNDSSRVFIREGSVWNWSKPTADLSMQSGGWHRGFGFLCVTGENSRVE
                     MNGRSVSINNDDSGVYLFDGGALNCQTLTVGTGSHGSNGFVQVRNGMLCAQSFNLGST
                     ALTAYKPTLTLAGETTLVAVTNTGATANFTCYERTGATIAFEIPADGYRTNGVARAPL
                     LLDNLAFNTTRSTSYPNDGPTSLRIACMAWAVAHPNATIPLVSIRKAGGDKADLEALT
                     NGFVFVDVPTSVIEAGNAPVLSIDEGGTTLFITAPESMGTNPTPPEFTVESVSSGANG
                     IRDIFLTISTYGFLSTQATLVELTVATNETFSGAVVTNLATQIEAPTPYSAAFKLPGC
                     LQRQHYWCRLRVVNDNDLDATVPFEFTDEGWEETYVWANAADGHWDTLANWMTGGAAA
                     TELPHGEDMLSSFPVGSYTVTLDSDSEIRSIGFFTAGTLEVPRVYTFDLNGKTLRTTK
                     VTQSVTGFSGTCPYSPAVPMSGQLVFKNGTFDASLSTSLSTFEDAELRVTDGATVYLK
                     LVMNGAARLIVDNGGFFRPQDHITLNANNRTYKTYGLIRVNGAGSKYGSWADLNVGGT
                     LSAVIVENSGLITVGSLNIGGNRNTPTAPCSNCVARADNGTFELSSSLNLGTANAECY
                     RPELRITGTNGFVRATGSGKISSFYENIGATIALEIPAEGFADTNGTARAPIHLNALQ
                     FVARGAGLADFGATTLEITARDWVKANPKQTIKLVELITPNAEALTKLKNAAVFTDTS
                     DATSPTLTVSEDGKVLYATAPVWPGFIMLFY"
     gene            19734..20525
                     /locus_tag="PK576_01120"
     CDS             19734..20525
                     /locus_tag="PK576_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60223.1"
                     /translation="MTIRQKVAFGLGCIGCAMAGAGIESARRALKVPSVPDMMQVERE
                     VVRLTRTPGQTVVVRDEALASEAEALRTRVAELEQALAARDAAPAAVAVSHLATNAAP
                     ARAGRPERESWEQRMERMRTEEPERYAEMQKRREEFRQQMEQRARERADFLDAVDVAG
                     MNDAQRENHEKLLALVARANELGMQLMQGGGRGEQGEALRREMGETMSALSTLYEMER
                     DYLLEATAKAVGYDGEDVKAFTEHIRHIMENTSPMPGFGRRRGGR"
     gene            complement(20548..21648)
                     /gene="ychF"
                     /locus_tag="PK576_01125"
     CDS             complement(20548..21648)
                     /gene="ychF"
                     /locus_tag="PK576_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOE60224.1"
                     /translation="MSLGVGIVGLPNVGKSTLFNALTHAQNAEAANYPFCTIEPNKAT
                     VALADPRLDALAQIVHPEQVVKATVDFIDIAGLVRGASKGEGLGNKFLANIRETDAIL
                     QVVRCFADDDVVHVEGEVDPVRDIEIVETELVLADLQSVEKRLDRIARQARADKDLRK
                     EVEAMERLLRHLDAGNPVRTFPREEADPIANTLTELQLITDKPVIYCANVGEIDLAAN
                     SGSVARVRAFAAEKGSDVVVISAKMEAELASLPVEDREAFLASYGLTESGLDQTIRMA
                     YHTLGLASYFTAGPKEVRAWTFRRGWAAPTCAGVIHTDFERGFIRAEVVAFEDYVRLN
                     GEAGAKAAGVMRVEGKTYAVQDGDVMHFLFSV"
     gene            complement(21743..21828)
                     /locus_tag="PK576_01130"
     tRNA            complement(21743..21828)
                     /locus_tag="PK576_01130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21793..21795),aa:Leu,seq:caa)
     gene            complement(21834..21907)
                     /locus_tag="PK576_01135"
     tRNA            complement(21834..21907)
                     /locus_tag="PK576_01135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21873..21875),aa:Gly,seq:tcc)
     gene            22135..23208
                     /locus_tag="PK576_01140"
     CDS             22135..23208
                     /locus_tag="PK576_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE60225.1"
                     /translation="MELRKACKYALAVPTSMGIRITPSNGQPVHCGGQFMMQVTSAET
                     NVASVAAYLGLPVKVLTTFVKDSPIARLIQDNLRSRHMDYEGVEVPQGGPWGYRHQIN
                     IADSGFGSRGPRVWNDRAGEVGRTLNVKDFDLERLFGDEGVQIVHLSGLIGALSPETG
                     TFCLELARAAKKHGTRISFDLNHRASFWKGRESELSAIFAEIASIADILVGNEEDFQL
                     CLGVRGPEAGGKDIKAKIDGFKGMIERVRKAYPHAQTYGTTLREVVDTNRHLWGALMC
                     AGDAWHVVEPRDITVLDRIGGGDGFVGGMLYAILKGWEPSKWIQFAWACGALATTMLV
                     DYALPADEEQVWSVWEGNARVQR"
     gene            complement(23361..24017)
                     /locus_tag="PK576_01145"
     CDS             complement(23361..24017)
                     /locus_tag="PK576_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOE60226.1"
                     /translation="MSDRTRELNLWTSEKKCRKLVENLARRHFTAVYCACAREAAEYI
                     LKQSADAHTVGFGGSLSVADLELTADLTAQGKEILNHGFPDLTPEQRVEIMKRQQTCD
                     LFLSGANAVTLDGCIVNIDGNGNRVAAMIYGPTKVIVVVGRNKIVEGDVSDAIRRIKD
                     KSAPPNAMRLGRQTPCAATGFCSDCDSPQRICHVTTILDSKPTLTDFHVLVVNEDMGL
                     "
     gene            24408..26138
                     /locus_tag="PK576_01150"
     CDS             24408..26138
                     /locus_tag="PK576_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE60227.1"
                     /translation="MRGRIGNVAAGVLLAAGLAQAGPTVFRRGMDRVSSLDPAMASSL
                     YAARAVQLVYETLLEYDYRARPYRLIPGLATHLPEVQSNGCVYVFTLHPEARFQADPC
                     FGPDGSGPPRGRPVTAEDVVFSLKRLADRRLASPGSWLVEDVIAGMRAFAETSETGSA
                     TDYGRPVSGLRAVDERTVRIELTRPMHQFIWYLAMAYTAVVPHEAVRCYGTDFGSRAV
                     GSGPYRLTDWKRNHQMSYTRDPEWRGWRSGAAAIDASDGTVPFDRVEYRVIDDVSTQW
                     LCFLAGELDFLGEVSRDNWDVVVDAAGGLTEPLRRRGVTLFAMPTLEVAYVGINMEDP
                     VLGKNRALRQALNCAFDRAAWVRFFNNRAVPCDGPVPPGTEGRLETPFPYAYDLDKAR
                     ALLREAGYPDGMDPRTGRRLELNLDLGRTSQDIRESTELMVSFLAQVGIALKPQYHNW
                     PAFLRRLSERRSQMFRIGWVGDYPDAENFLQLFYSRNVSPGPNRTNYVNPEFDRLYEA
                     ACAAEDAAGRNRHWAAAQRLIREDCPWIFLSYAKAYSLCNPSVLYYMPSDFPYGTEKY
                     LRTRAEREGI"
     gene            26186..26656
                     /locus_tag="PK576_01155"
     CDS             26186..26656
                     /locus_tag="PK576_01155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOE60228.1"
                     /translation="MVRYCYRGTAQIGRIGVVGTHKAVTRLLFEEDTPPADAEPGECP
                     LLREALRQITAYLEGRLRCFDLPLAPEGTPFMRRVWKRVLEVPYGTTASYGQIARAIG
                     RPGAARAVGMANGANPIPILIPCHRIIGADGTLIGYGGGLSRKARLLEIERSLF"
     gene            26653..27297
                     /locus_tag="PK576_01160"
     CDS             26653..27297
                     /locus_tag="PK576_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE60229.1"
                     /translation="MKDNANKTVAAPFSSVVNPMQPETGTAVESDASLLSAFAEGDAA
                     ALDRLVGRYRSALFSWLLGMTANRADAEDLYQEVWVRIIRHAARFHDVSFRAWMWKIA
                     RNLLIDFRRKRKPVVSLDVVSDEEDRPLIEQLADTTAGPVERVERGDLKACVMRAVKL
                     LPEVQREVFLMRVQGDLPFHEIAKTLDIPLNTALGRMHDAMTKLKRMLAEENLR"
     gene            27294..27722
                     /locus_tag="PK576_01165"
     CDS             27294..27722
                     /locus_tag="PK576_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60230.1"
                     /translation="MKPQNTFDEIAALERDAAALAPQVREAFRWAGGPSATVEEAIRQ
                     EAARVAARARVRWPVWSLRRLQVVAVACLVLLLAAGGLLLTSPQVYRAKAVRQTAHVG
                     TRPAGGASEDAAGFARLLLNIQGLDEDSFVTDGASEALWL"
     gene            complement(27688..28041)
                     /gene="rsfS"
                     /locus_tag="PK576_01170"
     CDS             complement(27688..28041)
                     /gene="rsfS"
                     /locus_tag="PK576_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOE60231.1"
                     /translation="MTINSEKIALETASALESKKADAIRIFDVRGMSSVTDFYVVATG
                     TSAPHLKALIAETQHRMKSLGVTAYRKSGEPDSGWVVVDYIHVVVHVFSPEARAYYAI
                     EKLWSTAKELPMPHP"
     gene            complement(28038..28664)
                     /gene="nadD"
                     /locus_tag="PK576_01175"
     CDS             complement(28038..28664)
                     /gene="nadD"
                     /locus_tag="PK576_01175"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOE60232.1"
                     /translation="MACIGILGGTFNPIHLGHLLIGRAAAEAFGLDRVLLLPCCVSPF
                     KIGAADLAPDADRLEMVRLSIQGDPLFQVSTLDLDRGGVSYTIDTVRALRAQYPNDRF
                     AFIIGMDTLCGLSHWHQADALLKLCEVITVERPGMDVRELPGEALSFPQEVRDRLLAN
                     VIRGPLFDVSASEIRRRIAEGKTIRYLVWHEVEAYIRTRGLYRTVKEM"
     gene            complement(28684..29784)
                     /gene="ugpC"
                     /locus_tag="PK576_01180"
     CDS             complement(28684..29784)
                     /gene="ugpC"
                     /locus_tag="PK576_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOE60233.1"
                     /translation="MAEVTLNKVCKRYDDGGLAVDRFTLTIRDGEFLVLVGPSGCGKS
                     TTLRMIAGLEEITSGTIHIGDRCVNAVPPKDRDVAMVFQNYALYPHMTVFENMAFGLR
                     LRKFPRAEITARVQEAAGILGLDGLLRRLPKQLSGGQRQRVAVGRAIVRQPKVFLFDE
                     PLSNLDAKMRVEMRGELRRLHRRLAATMVYVTHDQTEAMTMGDRIVVMRRGTIQQAAS
                     PMEVYRRPANRFVAGFIGTPPMNVLRGRLVRQPDDTLLFQYAPDARLPLPPVAMSSLA
                     QAADREVLLGFRPEALGADDAAGGVSLRGTVDGVEPMGAETFVQLVAEGGTRLIARVH
                     PDRVLTPGTVADFPLDMRHALFFDAETEHVIG"
     gene            complement(29805..30107)
                     /locus_tag="PK576_01185"
     CDS             complement(29805..30107)
                     /locus_tag="PK576_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOE60234.1"
                     /translation="MNSCRPAPRMENRDAAPAAQEPAYPGEFHFRILTETATHAETAL
                     AARIAAYRVTSPLAVSRDSSTGRYRAFCVSVRLEDREEHLRLDAEIKQVPGVRLVL"
     gene            complement(30104..31801)
                     /gene="recN"
                     /locus_tag="PK576_01190"
     CDS             complement(30104..31801)
                     /gene="recN"
                     /locus_tag="PK576_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOE60235.1"
                     /translation="MLNHLSVKNLAIVEEAAVTFGPGLNIITGETGAGKSVLMGALDL
                     VLGGRADKSAIREGAAEAWVEAGFALADTREVDALLESAGLPPCEEGALLVRRSVSAS
                     GAGRCLVNDSPATAQTLRRLGNLLVDIHGPHDHQSLLDSAFQRDVLDAYGHCVPQREA
                     CAEVWQTLQTLEARLDELQRDAPNIEAETERLRFIAEEITHAQLTEADEEDLVSRHAQ
                     AAHAEQILALGNAVVEGLTDGEPAVFSGLVAVQTQLTELARLLPEAADWLAEARNAAV
                     QIQELGRAVTDCLSRIEADPERLSHLEERMALVQRLKRKYGSSVSEVLATLEKSEARL
                     QDLATRTEQADALRKEIADATQTLTRRAAALSTVRARAAGALARSITQELHDLGFLKS
                     GFDVELSPCPPGPNGADTVSFGFAPNPGEPMRPLKAIASSGEIARVMLAVKAVLALHD
                     RIPVLVFDEIDANIGGEIGRVVGQKMRRVAETHQVISITHLPQSAVYGHRHVVVTKEV
                     VRGRTRMRITTLDDAGRVDEIARMLGGKGLTSVIESHARELLASASIQTTTPAKGVS"
     gene            32055..32807
                     /gene="tpiA"
                     /locus_tag="PK576_01195"
     CDS             32055..32807
                     /gene="tpiA"
                     /locus_tag="PK576_01195"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE60236.1"
                     /translation="MRKKIVAGNWKMNKTASEAVALIEEIRKETEGVSAVEIVVCPPF
                     TNLKDAAAACAGTHVMLGAQNVHWEASGAFTGEISGAMLKDLGVQYVIIGHSERRQYF
                     GETDETVNRRVRAALAVGLIPIVCVGETLAERDAGQMESVVVRQTKEGLADLGEDLGR
                     VVIAYEPVWAIGTGRTASPAQAQEVHALIRRTLAEIAGGDVANTIRIQYGGSMKPENA
                     RELMSQPDIDGGLIGGAALKADSFAAIIRAGM"
     gene            33213..34817
                     /locus_tag="PK576_01200"
     CDS             33213..34817
                     /locus_tag="PK576_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE60237.1"
                     /translation="MERETHLVEGSAAQMRVPQEALRRRALEEGVAAYVARERLVPPL
                     TAAEMLAHARAATASENLDDAEAVFAAVLLNNAVWADVVAGIPYERRLLLLPLCLARN
                     EVCRAERDGLGLLCAQCGNCPIGLIQAEADRLGYVSLVAEGTTVVTQLLAGGKVDAVI
                     GVGCMDSLRRMFPIMNAHAIPGQGIPLLTDGCQATEADVAWILRVLRASRQGGVSGVT
                     EIEAVADAVRNWFTCEELAAVLGSAETEAERIGREWLLIGGKRWRPLLTAAVSEALGG
                     ARDAIRPAALAVECFHKASLIHDDIEDQDTERYGVPTVHCRYGVPAAINAGDYLIGEG
                     YRLLAEAAFPDDLRARMVTAAARGHRELCLGQGEELAFSRQPAPVSESAVLRIYERKT
                     AAAFEVAVLLGAIAAGADADTCARLSEFSRAVGTAYQIGDDMEDFFSARGRAADLLAM
                     RPTVFLATACTCDNAEVAAALDSVWNADRAGRQRLIDAITQAGLHTRVRLLYEHYRHE
                     TERTIAGIQHTGLQRLLRHVMGRMLR"
     gene            34847..36298
                     /locus_tag="PK576_01205"
     CDS             34847..36298
                     /locus_tag="PK576_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOE60238.1"
                     /translation="MRIRQAIPFCVTCLAAVCAVGTLAAWLARESAVDASLRLATADG
                     SGAEAAAITESDARGTDLTGCFRANREIPRGATTGNWPGFRGAGRDNVARRAGKLLTA
                     WPETGPAVLWQIPVGDGHAMPALANGCLYLLDYDEARGGDCLRCLNADTGQERWEHLY
                     AVKTKRNHGISRTVAATDGRSVVTIGPQGHVVCLDADDGTYRWGFSLSERYGSRVPLW
                     YVGQCPLIDGDRVILAPAASGVLLCALALSDGKTVWETPNPHGYAMSHSSVMILETEG
                     RRHYVYAGIGGIAGVGAEGAERGTLLWHTEAFRPSVVVPSPVPLPDGRFFMTAGYGSG
                     GALFRVSRVSDGWRAETLFKTDRKTFASEQQTPVFLNGLLYTVLPSDGGGERQQLVCM
                     TLEGQRMWASGRDHTFGLGPYVATPEGMLLLLNDAGTLTLARVGRDGFVPLARHALME
                     GRGRDAWGPMVLADNRLYLRDSKRLFCVQVGAP"
     gene            complement(36311..36532)
                     /locus_tag="PK576_01210"
     CDS             complement(36311..36532)
                     /locus_tag="PK576_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOE60239.1"
                     /translation="MDEQIKAKLEELRGMLQADGGDMELVSITDKTVRLRLRGACGSC
                     PHATMTLKQGIERVLREQIDPEITVERVV"
     gene            36566..37333
                     /locus_tag="PK576_01215"
     CDS             36566..37333
                     /locus_tag="PK576_01215"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOE60240.1"
                     /translation="MPENTGCVKLRLTGSGGRAKVGFVNHKAETTVLLANGAYPVHPF
                     PVDALRSARRIVCCDGAADRLSQAGLEPAWVIGDLDSLSPAARVRWPGRVVAVSDPES
                     NDLTKAFRFCVSRGWRDLVIVGATGLREDHTLGNLSLLPDFAQEAEVLLLTDTGTFRP
                     MLRSARLRAHPGQQVSIFSFDPRTVITSEGLRYPLRRLRLSRWWQATLNEACGDEFDL
                     IFEGGPLLVFQTYEIRPNRAPGGGDGEMAGQHESNSV"
     gene            37314..38204
                     /locus_tag="PK576_01220"
     CDS             37314..38204
                     /locus_tag="PK576_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE60241.1"
                     /translation="MNRILFEPGELCADGTVTLADARAEHILNVLGLGVGGVVRSGVV
                     NGSAGTSRIIGTEGKRIRLQTDHTQPAEPSGFDLLLAAPRPKVMKRLWPQLAALGAGR
                     VVVLNAAKVEKCYFSSQWLDPARIRPLLIEGLAQAGTTMLPEVRVRQRFKPFVEDELG
                     TLFPTPLRLLAHPGPVVGLAASEWPSGTRPLIAVGPEGGWTDYERAMLTERGFRPFSL
                     GPRTLRTDTACVALIAVLAWLRDSSRWSGGADESRVLSGRLGIGQVAGPSCTINAERR
                     TENHETRTLGSLSSEARYAD"
     gene            38152..38955
                     /gene="rlmB"
                     /locus_tag="PK576_01225"
     CDS             38152..38955
                     /gene="rlmB"
                     /locus_tag="PK576_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOE60242.1"
                     /translation="MKRERWVRYPQKRDTPTECRDWIYGRRPVLEVLKAGRRHLYEAV
                     LPPAGRDAPEIAEMRDLLLARGVPFRTLERSELDESCEGGNHQGVAVRTGGYPYISFD
                     QVLDDVKTDTNAVVLILDHIEDPQNVGSLLRTADAAGVTAVLIPKDRAADVTPAAVRA
                     SAGASEHLRVVKVVNLVRAMKELQACEVWITGLDFGPDAKPYTSIDFKGRAGLVVGSE
                     GRGLSRLVRETCDFIASLPMKGRVESLNAGVAGAIALYEVVRQRGQASR"
     gene            39013..39762
                     /locus_tag="PK576_01230"
     CDS             39013..39762
                     /locus_tag="PK576_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle suffix domain-containing
                     protein"
                     /protein_id="HOE60243.1"
                     /translation="MSMFGGTGGYFRLDSWILANIVQLGTQRFCKRFLNRTNDPCGRQ
                     YDQMAQAARSGCSNIAEGSARHATSRETEMKLTDVARSSLAELAGDYLNWLLQQEQVP
                     WGKATPESRAVYSVRLDKADYGEDVIHDACAHILAQKRKFAQWLESESDVTMANALLI
                     LIARVINMLNHQMESQGEAFKAEGGFREKLTGIRVEARARQQAAPVCPDCGKPMARRK
                     ARSGKNAGREFWGCTGYPECRGAREMEGGEK"
     gene            39863..41686
                     /locus_tag="PK576_01235"
     CDS             39863..41686
                     /locus_tag="PK576_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOE60244.1"
                     /translation="MRTPLESLVPRPRRIESGPGVLALGEAGVIVGAGAPDDLRPALR
                     VLEAALGSGWPLSEARASSPAGGVRLVFRIVPQVAGPQSYCLRVTSAGVEAGAQDRAG
                     LFYAAQTFAQLARLSGGVLPVCEIRDAPDFPVRGVMLDISRDKVPTMDTLFGLVDMLA
                     GLKVNQFQLYTEHTFAYRAHEEVWREASPMTPDEIRALDAYCRGRCVELVPNQNSFGH
                     LERWLTLPRYNGLAELPQGGAPLPWGGVHEKPASLCPTDPRSVDFLAGLYDDLLPNFT
                     SRLFNVGCDETFDLRGNGRSQALVRQVGEGRVYLDFLKTIHGLVTARGKRMAFWGDIV
                     IRYPELIAELPRDVLALEWGYEADHPFDAHCERFAAAGVPFYVCPGTSSWNSLAGRTE
                     NMLGNLRSAAENGLKHGACGFLIADWGDGGHWQPLCVSFAGFLYGAALSWHVEGNREL
                     PLAAALDTRLTEAGRGQLLLGLGDVYRLCGALRGNGTELFQILSKPQSRPVAQGVTGK
                     TLCAVLSRVEELERSMLTAQRSVVEQETEQVIRLLRAACHRGLALLDGSVRRAATRRA
                     LRTETDDLACAHARVWRLRNREGGLADSLAKLTRHRQEYAE"
BASE COUNT         7715 a        11994 c        13817 g         8226 t
ORIGIN      
        1 acgcaagcta accatcacgg ttgagaggga tcgatccgtg gttgtacgcg cgcctgtggg
       61 agtttccgac gaaaagatcc gcgagatcgt cgaagccaag cgtttttgga tcttcgagaa
      121 actgcaccat gtccagaaat acactccgcc tcacccgccc ggcaaggaga tggtcagtgg
      181 cgagtcagtg ctttatctgg gaagatcctg ccgaattgag gtggtagaca ccgatgcaac
      241 ggagatcctt ttcgatcacc ggtttgtcat tccacatagc cacgcatcgc ggcgcaagga
      301 agtcctgcgc gaatggtaca ttgaacgtgc caaagcaacg attctgccta gagtgcggag
      361 atttgccgac aagctcggag tcgactacaa gcatgccaaa atcgtcgaca gccgctatcg
      421 ctggggttct tgcaccgtca aagataacgt caactttaac tggaggctca tcaaagcccc
      481 tatgttcgtg atcgactatg tcatcgtcca tgaactagcg catttgatgg agcccaacca
      541 cacgccgcgt ttctggaaca tcgtcagtgc ccagtcgccc aagatggaaa aagcgcgggc
      601 atggctcaga gacaacgggc aactcctcga agaagacatc tgagcaatga catgcggtgt
      661 gtctcaaacg agttgggaag cgaagcggtc aagggcttcg tggaccgggt cccgcggcgg
      721 gtggtttgcg tgaatcaggg atatctgggc ttggtcgaag gcgatcagcg cgtcctcccg
      781 gcttcgggcg atgtgggtgc gcatccagcg ggttgcgcga tcggcgtcac cgcgccggat
      841 ggcgcgctca atctcagcgt gatggtgcca tgcccaggcg acgtgattga gatcgcgccg
      901 atggctgtag tggccgaaga agaggttccg ctgataggcg tgggtcacga tcttgatggc
      961 cagcgcgttt ccggcggctt tgagcaacag caggtggaac gagaggtcgg ccttgaggaa
     1021 ggtgacgagc ggttttccgt ccagaacggt tttgtttcgc ttacgcaacc cttccgcgac
     1081 tttccgcatg acatcgcaag tttgacggca ttcggcgcgc tgttcccggg tcaggttcct
     1141 gaccgcgcgg tcaagggcga agcattcaag ggccatgcgg acttcatacg cgtcgatcag
     1201 ttgacggcgg tcgagccggg cgacatacgt gccgctgcag gggatttggt agaggacccc
     1261 ttcctcgatg aggcgacgca cggcctcgcg caccggggtc cggctgatcc cgcactcggc
     1321 agcgatcttc tgttccgaca gtttctgtcc ggcgagcagt tcggaagcca taagcttacg
     1381 ggtgatggcc cggtatgcga tatcactcaa tgtgggtgct tgtgccacac gatcctcgtt
     1441 gtttgccatg cgtccgggtt gacggacgcg atggcgttat tgatatcaca aatcgccggg
     1501 ggtgtcagcc atcacgatcc ggttcccggg cagcgaccga aaaatgcggg accgacggga
     1561 tgcggcgcgg atcagaggat ttcgcatttg ccgccggtgc aggccacggt ctgggtgacc
     1621 gtggtgttgt cctggcgctc gatgagctgg gtatagtcca ccggcgcata ggtgcgtgtg
     1681 aggtcctccc agtgtttgca cgcattgacg cgtttgaggc aacgggtcat tttcagcaca
     1741 tcgttcccga aatagttccg ggcgaacttg cgcgcgcggc gcacccaatc ctgtttggcc
     1801 tcgcgcacgc cttcgatcgc ctcgtagacg cgtttggggt aggtcttcgg tgtgggctcc
     1861 tccagatttt ccccgcgccc caagacacag tcgcaggcgg cccacaggtt atcatcgaac
     1921 gcatgcaggc cgtccacgat caggccggag gcgagcaacg cgccgacacc gtagatttcc
     1981 acaatctcat cggcggtgag cacttcgcag aagggggcct gcgggtagtc gaggtctccc
     2041 ccgtccggca gcagcgagac ccccgcgaac acgtggcggt tttcgtagat gaaatcggcg
     2101 acactctccc attcgctgtc tttgacggaa atggtattgg agacgttgtg gctgagccag
     2161 ggttgaacac actgttcagg gcgtttgccc gcatcgatcc agttttcttt ggtgagcttg
     2221 accttgttca gcaactccac ggcgtccaca tcccgcttgg tgagcgcgtg cggacttgcc
     2281 tccacgcaga atgtcagcac cacatccgtg ccgttgggat tccagaccga tttttccact
     2341 gctttggggt tctgcgcgcc aaagaaatca acgagcagtt catcctcgtt ggcctgggcg
     2401 cgccggaaat aacgtttcgc atggtggggg tggatccccg acgatgtgcc cagaatgctg
     2461 ctggtggtgc cagcgggttt gacacaggtg gttcgtgcgg cggggttgat gccgatccgt
     2521 gcggccacct ccgcattgac ttccagaatc acctttgcca tgtctcgctg gagctgcgga
     2581 tcaaacgaga cgtccgggtt ttccatcatg ccggtcatcg aaatgccgag cagcgcctca
     2641 cgtttcgcaa tgcgcgtgct gacctcgccg agatattcga agtcggtata gtccgcttgc
     2701 agggttccca gaatggcggc cgcgcggcag gcttcgcgga atttgtccgc gttgtccacc
     2761 agccccatgt tgatctcgca caggttgcaa aaggcgaatc ccgtgacgcc gtcgatctgc
     2821 ggatagagtc cgatctctcc gcaggggttg aaggtcatgt cgagatggtc ggtccaaacg
     2881 aatccgggct cgccgaactc cttgaccgat ttcatcagcc ggttgaactg ttcgcgggtg
     2941 gtttggttac gcagcaggac ggcggaattg ttggaccggg cgcgctgcgg gttttcattg
     3001 aaccagtttc cggtcttggc cgtggccatc agttcgtcgt ccggcgaaaa gaggcagatg
     3061 gtggcgctgc gtcgcacgcc gcccgaaagg accgcatccg atgcatgcat gaggatgtca
     3121 taggcgtcga ccgagcgtag acggccgtgc ccctcttcga tgcgccggtc gaccagtgtg
     3181 cggatcattt ccaggctgcg gcgtagcggt tcgggccccg gcgcgcgccc catgccagag
     3241 gagatggggc ggcctttggg acggatttcc gtgtagtcga attggacgat ctgtcccgca
     3301 tattccggaa agggctgcgc gtccgtgaaa taactggaga gcagtacacc caatgcgtcg
     3361 gcccaaccct cgatggtgtc gggaatgatg aaggtggcgt gtcccgctgc gggtttgcgg
     3421 atgcccggga gtttgtcgat gtggtggggc tgcaccgaaa accccacgcc gcaaccgcac
     3481 aacagaagcc agagcgcctc ttggaaaaag cgggggcggt cacaatagga ggtggtgcag
     3541 ttgtagatgc gcgcgttctt gcgcaggatc ggggggccgc cgaattgcat cgcgcgctga
     3601 ctgcccagca ccatgcgttt tttcatcgcc tgttcacaga ggctcagcag atcctccagt
     3661 tcgatcccct tggccgcaaa atggctgcgg tgcatcgcga tcacccggtc gaccgcttcc
     3721 tcgaaggtct cccgccgcat tttttccggt atgtaccggc tgtattttga ggtgaaaaca
     3781 taattcttca acgattcgat gctcatggat tccctgtacg taagaaaaga aaaggtgact
     3841 gaaatgatgc ccggcccctg tggtctgatg acatcaggga gctctatata tagtggttgt
     3901 cacccgggaa gtccaccata ttttgtggtt tttcgtaact cattggctct caaagaaatc
     3961 gggaagcagt ttttgggctt tctccgtctt gggggcgggg gtatcatgaa aaacacatgg
     4021 ggaacgatca gagagaggtc cgggaggagg ctctgcaatc cgacgccgca cgaggtgacg
     4081 agacgggggg gagcgcaccg gttcgcggga ggcttgcgcc gagtccgacc ggcgcgttgc
     4141 atctgggaaa tgcgcggacc ttcctgattg cctggctgcg ggtacgcagt cgggcgggct
     4201 ccgtgattct gcgcatggag gatctggacc acccgaaaat caaacgtggg gcggcaaggg
     4261 aaacgctgga tgacctgcga tggctgggat tcgactggga tgaagggccg gattgcggcg
     4321 gccccttcgc gccgtacacg caaagtgagc gcagggcgta ttacgcacgc gtcctgcagc
     4381 gtctcattgc gcgggggcgg gtctatccgt gcatctgttc gcggcgggat gtcgaggccg
     4441 ggcaatccgc accccatgcg gaggacgggc tcttttatcc cggcacatgc agagggcgct
     4501 tcgcggatta tccggacgcc tgccgggtgc tgccaacggg gcgtttgccg gcctggcggt
     4561 tccgcgttgc ggcaggggag agggtggcgt ttgacgacaa gtttcacgga cgtgtcgaac
     4621 aggatgtcgc ggcggcgagc ggcgacttcg tgctggcgcg ggatccggac ggcgcgggct
     4681 acatgctggc ggtggtggcg gatgacgcgg cgatgggcat cacggaggtg gtgcgggggg
     4741 atgatctgct gcctgccacg ccgcgccaaa tcctgcttta ccgcgagttg gggttcccgc
     4801 cgccgttgtt cgtccatgtg ccgctggtgg tgagcgagga gggccgccga ctggccaagc
     4861 gacatggcga tacgcggatc cgcgccttcc gcgaggcagg ggtgcctccg gaaaaaatca
     4921 tcggtctgct ggcttggtgg tgcggttggg ccgaatgggg cgagatgctt tcactgaatg
     4981 aggtgctgcc ccggttcgac atgcgtcggc tgccgcgcga gccggtcgtg ttgacatctg
     5041 cgatccggcg ggagttgggt atccgctgaa ccgccgcagg tccctgccgt gttctgcgga
     5101 caccccgggg atgcgtccct accgtgcggg ctccgtcggc cacgtcaggg ccagaagagg
     5161 cgggggttgc ctgacatgcg ggcctcgcgg agcgtggccc ggcgcagggc gtcgaaccgc
     5221 ggacgcattt cgctcaggct gtctcccccc agcttctcga tcagcgcgtc cgccagcacg
     5281 atggcgacaa ccgcctccag caccacgacg gcgcggggca cagggcacac gtccgaccgc
     5341 tcgtaacagg taagcgaagt ttgtccggtc gcgagatcca ccgtttcctg ccctttccgg
     5401 gtggtcggga tgggtttcat cgccgcgcgc aaccagatcg gcgccccatt ggagatgccg
     5461 ccctcgatgc ctccgcaccg gtttgaaccg cgcagaagat tgccctccgc gtcgggccgg
     5521 atcgcgtcgt tggtgagggt ccccgtctgc cgcgcgctgg cgaacgcgtc accaaacgcc
     5581 acgcccttga tggcaggcac actgccgagc gcctgaatca ggcgggcatc gagacggcgg
     5641 tccgcgttca cgtgcgaacc gaggccgacc gggatcccgt ccgcgacgac ctccatgatg
     5701 cccccgagcg tttcgcccgc gtcgtgggcc ttggcgatcg cgtccgacat ggccgcgtcc
     5761 gccaccgggt ccgggcactg gacagcagag gcgcgggccc ggctgacgcg ctcagcgaac
     5821 gcgagtccgt ccagccgtgc ggtgtgctcg ccgatggcgg acacataccc gccgaccgtg
     5881 atcccgaagt tgtcgagcag ggcgcggcag agggctccga ccgccacacg catggcggtt
     5941 tcacgcgctg aggcgcgttc cagcgctggc cggatgtcgt cataaccgta tttgacggct
     6001 gcggcgaggt ccgcgtgacc ggggcgcggc gtggtgtacg cggggatctc ccgacctttc
     6061 cagttggcat gatcacggtt cgggatcagc agcgcgatcg gcgcgccggt ggtgactccg
     6121 tccatcacac cggacagaat ctgggcggcg tccttttcga tggccatacg ctcgcccgag
     6181 cctttcccgc actggcgccg cgcgaggtca agggcgatct gcccttcggt gaccggcaga
     6241 cccgccggca tgccttcgag gattgcggtc agggccggtc cgtgggattc gccagcagtc
     6301 agatatcgca gagccataga cagttccgtt ttgttaggga tacggtgttt ctttccagtg
     6361 gcgtatttta accggaaggc cgggcggaat gcaaggtgcg gcacgaaaac cttgtgtgca
     6421 tgggcgcgtg tccgttaaaa tgtttttctt tcaacgcaaa ggagagatat ggccaaaaca
     6481 tacaaaatcg cggtgctgcc cggtgacggg acaggtccgg aagtgatcgc cgaagggtgc
     6541 aaggtgctgg acgcggcggc gcagaagttc gggttcaaac tcgacaaggc gttttatgat
     6601 ttggggggta tgcgttatct ggccatcggc gagacgctga cggatgcgac actggatgag
     6661 ttgcgccagt ttcaggcgat ctatctgggg gcaatcggtc atccggacat caagccgggg
     6721 attcttgagc agggggtgct gctgaagctg cgctttgcgt tggatcagta catcaacctg
     6781 aggcccgtga aactctatcc cggtgtcgca tgcccgatca aggacaaagg cccggcggag
     6841 atcgattact gcgtgatccg cgaaaacagc ggtgggctct acaccgggat cggcgggctg
     6901 tcgcgtccgg gcacccggga cgaggtggcg gttcagcaga tgatctacac gtacgcccaa
     6961 gtggagcgct gcctgcgata tgccttcgaa tacgctcggc ataacggcaa aaaggcgcgt
     7021 ggtaaggggc cagagaacaa attggccctg atcggcaagt cgaatgtgct gacgcacatg
     7081 tacgcgctgt ggaaccgcgt gttcgacgag gtcggctcag agtatcccga tgtgaagcgg
     7141 gagtattacc atgtggacgc gacctgcctg tacatggtgg cctgtccgga gatgttcgac
     7201 gtgttggtga cggaaaacat gttcggcgac atcatcaccg atttggcggc gatcacgcag
     7261 ggcgggctgg gcgtggcggc gggcggcaac atcaacccgc agggcgtcag catgttcgag
     7321 ccgattggcg ggaccgcgcc gatgtggacc ggtaaaaacg cgatcaatcc gattgccgcg
     7381 atcggcgcgg gcgccatgat gctgaccgcg ctgggtgaaa cggaggctgg ccgcgcgatt
     7441 gaggacgcga ttgcgaagac cacgccgaaa atgaagagcc agatggcgaa tgagatgggc
     7501 ttcaccacca cgcaggtggg tgatctggtc gccgaagcgc tctgatctgg attcacttgg
     7561 tgctgagcgc tttgcgcaac agcgcttcgg tttcccggat accgggttcg gcatccaggg
     7621 cgctctgcac catcttgcgc gcctgctcct gcgggaagcc gagggagccg agcgcgagga
     7681 tcgcgtcgcg cagcatgatg gtctgttcgg aggtgcgggc ctcgcccgag cgggcggcca
     7741 gtgccgcgag cgggtcgatt ttgtcccgca gttcgacgat gatgcgttca gcggtttttt
     7801 tgccgatccc gtggatcgaa ctcaaccgtt tcacgttgcc ctccgcgatg gcgagactca
     7861 ggtcggccac ggtcaggccg ctcagggcgc tgagcgccag tttcgggccg atgccgctca
     7921 ccgtgatcaa gcgttcgaac atcgccttct cttccgcctg catgaatccg aaaagcgtgc
     7981 gggcgtcctc gcggacataa tcgtgcgtca gcagtcggca gggggcaccg ctaccgggga
     8041 gccggtcata ggtgttgagg gagatgaaca cctcataccc cacgccgtgc acgtcgagta
     8101 cgaccgacga gggggttttc tcgaccacgg tgccctgaag aaaagcgatc acacccgcgc
     8161 gtcctcctcc agtcgcggcg cgtgcgtcag cacgtagttt tcggcctccg aacaccagag
     8221 cccgttgttc cgtccgcaga tttcatccag cgcggcgaaa aacggctcgg tccgtccgcg
     8281 cgcggcgaaa tccgtgatgg cggtcatggg catggagacg tgggtgtaga ccagtttttt
     8341 gccgccgggg atatcgggca gcttcatggt cgtctcagcc acggagtcga tgccgcccac
     8401 atgggtgatc atgaccgacg gcgtgatcag accctgcgcg gcgtaatcca tggcatcctg
     8461 caggtcctgg gtgttgccgc ccgaggaacc gaccacatgg tggcccatgt agtggacatc
     8521 atagaaattg atggccgcct tgaaatcgga gcgcgccggt cccgcgaaga agttcaggca
     8581 tccgagatac gccaacaagg ccgagccttg ctcgatcaac tcgggcacgg gggcgaaaac
     8641 catcacatcg tcaaacccct gattcgcgtt cacggatcgg atctgcgcga ccggatcatc
     8701 ctgccccgcg gtgttgatgt aatgcagttc aaccccgcat gttttggcgt gttcaaccgg
     8761 gaaaagctgc gcggcgcggt cgaggcgggc ctgatcgata tccgtgatga ccaggcgtcc
     8821 gggccggtgt tcgggcccgt gcagggccac gtcgatcgcg ctgagtccca tggggccggc
     8881 ccccgctagg atcaccatcg agcctccgtt tttaattccc atctgatgga cgtactcgcc
     8941 ggccttgaag tggtaactgc aacggtacgc gcccacgatg caggagaccg gctcggcgat
     9001 cgaggacttg tagtaggcat cgccgctgta gatgagcagg cagtccagtt ccagcacttc
     9061 ccgcggaatg atgatgcgcg tggcatttcc gccggtgtag gggaacgaat atcctggcgc
     9121 acggtgctcg tgaccgggga tgttaagctg gggctgcacg ccgtacttct ggccgaccga
     9181 aaatttgtgt tgccagttct tgccgacttt caggaacttg ccgcagaatt cgtggccgag
     9241 aatgatcggg tggtccgcca catcatccgg cacgcgtttg tgccgggcgc cctgaatcgc
     9301 cgccttgtgg tcggacatgc agatgctgtt ggtcacgatg tcggcgagga tctcgtcgtc
     9361 tgtgatttcc ggcagcggga aacgctccag gcggaggtcg ttctccccgt acaggcgcaa
     9421 ggcaagggtg ttcattcgct tttcctcaag gtgcgggtta tgatggcgtc gggttcgccc
     9481 cgaaaaccac gttagcatag ctttccgggt ggggcgcggc aagttcaaag atatgccggg
     9541 cggggggcgc accatgaaac acaagaagct tcatgaaacg aggagtggtt gcaggtctgt
     9601 gaatggttct gcaaacacgc caaacggctc ggcaggaaga tctggcttta cgacgaatac
     9661 aattggccgg gcggcccgtg tcgcggttcc gccgcaggcc tggtttttgc gctgtgagac
     9721 cggggagcac cgaactgagg atcacccccc acacatcagt cgcgccgatt ttcggaaacg
     9781 cggaaacgcc gggagcgaaa tggaagaaac tctggctgcc gatgacaccc caaggcgcac
     9841 gcaccgggct tctgtccgcc ccggtgtggt gctggtaggc agggttccct gaacggtcgc
     9901 gggatggata aaaccgctct agcgttcccc gggggaggac tcggcgggtt ccgtcggcaa
     9961 cacggtgggg ggttccgcgt gcgtcgcggg tttcccacgg tccagccatg ttgcgagacg
    10021 tcggaaatcc tccggcgaga gcggtcggtc gctcaggccg aatgctggcc acaggcatcc
    10081 gcgaaccagc cagacgagga aagcgaacga ggcgagcgga aaaaccagtc cgttcagtat
    10141 tttgatggcc accagtttgc agaccgccat cgcgacctcc ttgatggaca cacgggcgta
    10201 ctctccgagg gaggcgagtt tttctttgag gatcagtgct ttgtctttca actcgtcagc
    10261 ggcatgaaac tgctccatgg aaaaagcgga accgatctgt tcgaacgcgg ttcgaacctc
    10321 ttcgcgcatt ggatcggtcg tgtggcgtga cagataggcg gttccggcga cggtaagcgg
    10381 caggatgagc agaaacagca gggccgccat cagaaagagg gcgccgaacc gacggaacgc
    10441 ctgacgccat acggggggga ttcccgggat catggcgaac agatgcccgg caagcagagc
    10501 actgaacgat aacacgagaa atggcgcgga cagcgcgtcc gtgcccgcaa gaacgcattt
    10561 ggcggtaacc aggtagacga ggctgatcat caggagacgc catgcgagat cgatgtagtc
    10621 gagcaacggc tgtagggtgt ctccggcttc gatactcatc cctgctttgg caaagacggc
    10681 tccggcctcg atgttgacgg tcgagccctc aatcgcatcg gcagccgctt tggcggtgcc
    10741 gaccggaatc atcaggcgcg cggcatcccg gatcgaggcg tcgagataaa tctggttgcc
    10801 ctgatccaac cgccaacgca accgaccggt caggccggtc agccagccgg aaaccgtgaa
    10861 taacaacagc ccgatcaaca gcgccgggat tgcgtatcgg cgtaaagaaa gcagggagag
    10921 cgtgtgtttc attttgcagt tcacgttcac tgtgttcact ttcttaatcg tcttcgcgtc
    10981 aatcatccag ttcgaccgtc cagcgcatgg tcgttaagag gctggaaaac cactctgcgg
    11041 ccgcatccgc tatgcgcgaa ccgacttgcg tggcctgcgc atgaggctct gcatgactgg
    11101 acgtgccgcg gacaacccgg gcataagtat aattgctctt cgccggatcc ctgtcgccga
    11161 cgggatccac gccggaaata cggtcgctgg tatagcgatc caacatcact tcaccgcgat
    11221 agccgagcat atcgtaaagc ccccaggtgt tcggaagata tcttcccacc gcgacattgt
    11281 tgtcgatttt attggcgata cgctgcagga ggttggtgtt ctgcgccgta gactctttgt
    11341 cgtagtaata gcctgtggtg ccggcgcggc aggcgacttc ccactgcgcc tcggtcggat
    11401 aatcgagtcg caggccggtt ttctgacggt agagcgcgag caggttcgta ggacacacat
    11461 catggccgta ggtcggccag ccgacgggat tgacgggatc acccaccggc ccgcgcatgc
    11521 cagccggata atcgttgtac ggatcatccg tatacgttgc acccacataa tcgcgcatac
    11581 cgcctggcat cggatactgc tgttccgtct cattgatcgt cgccattccc cagttcggat
    11641 ccctatgcct acggtttgca acatcccacg tgagccaact gtactgcccc tgtgtcatcg
    11701 ggaagactgc gatccaatag gccttggtga gtgaaacccg gttgaacacc gtctcgacgt
    11761 tgccgtcgcc cgtcttgacg cctgcctgcg gcggcacata gcggaacacc atcttcgagc
    11821 gcgcgtactg gttatcgttc gtcacgccga accacacgtc cgtgtagtcg atgtgggtga
    11881 ccgccatccc ttccgcctcg tcgtcagaga cgcggtattc aatcggatag tcgacgtagc
    11941 tctcaagacg cgcgtccccg gcatagatgt actcgcgctg gttcgcgtcg cccgggcgct
    12001 tcgtcaagtc gatgatcatg aagcggacgg ggttcgctga cgttgtgatg accgtgcgca
    12061 gggccttcgg gcgcgactca agacccgaca gggcgaaatc gaagacttgg tgccgacgcc
    12121 ccggcagacg gacttcaccg ttgcccggca gcgacgcggc cgcgactgtc aatggcgtct
    12181 cgccgtcgta gagcgccacg gttacgggaa atcctccgta gacgtccgaa tcgagcgtat
    12241 agtcgatatc gaccttgtcc tgccacggcc agcgcggcga aacggtgatg tcggtaatcg
    12301 tcgccgccag tgtcgtaagt ccggcgaaca gggaaagggc gaaaagcgat ttcttcatat
    12361 cagattctcc gctttcttca gtccacgttg atgacgagac gcacgcggtt gtagccgcca
    12421 tcataccaat tcacgtactt ggttctcgaa agcccggtcg gacgcgatcc acaggcctcc
    12481 agcagaccgg catgatgtcc accgcccttg cagatgcaaa atccgttcca ggtcgtgtac
    12541 gcgcccacgg gatccgttcc cgacggccag acccagttgt tgacctgacc tgcatcgctt
    12601 gatttggcgt tatcgagaac gccctgtccc cagccgagca gatcgaagag gccccaaccg
    12661 ttggcaagct tcctgaaggg attgaagttg gtgccgccct cgtgaccgag cttaagcagc
    12721 agatcagtgt tctcggcagt cagattaacg tcgacgtagt agaagcccgt cgtgccggcg
    12781 cgggcggcga cttcccattg cgcttccgtc ggctggtcaa aggcaaggcc atatttgatg
    12841 cgcgcctgac cgatgaagct cgtcgggcag acggagtggc cgtaggtgat ccagtccacc
    12901 ggatagaccg gatcgttgtg cggaccgcgc agatcgtcca gatagatctc cttggatttc
    12961 gactggttct ccgcattgcc atccgttccg gtatcggtct gccacggact gccgtaacgg
    13021 atataaccgt attggcctct tgaaagcaga ccgacggcga tgaagtacgg tttggtgaag
    13081 gtgacggaat tgtaggtcga cgagacgctg gctttgccga cgcggtagtc acccggcttc
    13141 acgagacgga acgccatctt gttgatcgcg tatggcgagg ccgtcttgcc gccggaagag
    13201 acggtcatgt tcgtaaggga aaggaaggcg tcggaatact ggatcgttgt ctgcgttggc
    13261 gttccgttat ccagatagtc gacggtctgc acgaacgtct cgaggcgcgg atcaccttgg
    13321 tcgatatact cgacaagccc gtcctcgtcc acctgtttgt cgagattgac gatcaggtag
    13381 cgcgtcggtg tcgccgaaat gtcaaacccg atacggaagt gctgtacggc gacgatgttg
    13441 gtaagggcag tcttggaagg atcgaacgtg agcgtgtagg tgcccgcact gtagaccggc
    13501 ttgaggttat cgaaggccgc cgtcccgtta agcgccactt gatgattccc gtcgtatatg
    13561 gcgaaatcct tcaggcggta gaaatccccg cccgtctcgc cgacggtaaa ggtgatatcg
    13621 atcttagggg agaacggcca gcgctgcttg accgagtcga tggacagcgt gacagcggaa
    13681 gccgcagaaa cggcgctgag cgccagaacg ggaaccagat gcttcatagg acgatccttt
    13741 cctagaagaa gagcacaaca aacccgttac acacgacttt gagataggcc gaaaaagccg
    13801 gtgaggaagg ctcggtctcg aagtcgagct ccgcctgccg gactccgggc tgactgaacc
    13861 gcaaatagga gtagccgtcg gaattgtcca gcgcgatctg ctcggcatcg atgctgagcg
    13921 tggccgcgcc cgcaaggcct tcggcccatg ccgcacgata gggtagcacg cagcctttcg
    13981 tgaccttggc ctcgcccgca tcattccagc ccttcagttc cacgccgccg aacgagccga
    14041 gattcgcgta gagctgcgcc ttctgcacca cgttgttatt gaacgtcaac gtcacgtcat
    14101 agacggtgtc ctcgaccgga acccccatgt cccaactgca ggaacggacg gcggtatctg
    14161 ggaacgtccg gctgaagaca gcattttccc tgccgttggc gacgatcaca agctccgccg
    14221 acgtggcgcc attgggccag gaccagttga gctgcacggt ggtcgcatgg agctgtcccc
    14281 agtcgaggtt actgccggca agcggcgcga tgatcttcat cgtcggcgaa agcgcggcat
    14341 tggcagctaa gacgaaaccg atcacaagac tgacaagaac ggagcgaaca caagaacgtg
    14401 atttgatcat catcgaattc ctcctagtgc gttgactgcg attatagcat gagttcaccg
    14461 tgtggacaat gggcttcgcg tcttttgcac ttcacgcgct ctccggtttt tgacgggttg
    14521 cgcttgctct ttcttaaaaa accatattat agtatacctt ttcattcacg gaagaaaggg
    14581 atgtcggctt tgtctgtacg ccggtaccgg ctcaagtgtc tggcgcggct caaccgatcg
    14641 aaaaaacaac actggggaaa gaaggagaca tacgatgaag aagtggatgg tgatgttcat
    14701 tgccgtcgcc gcgatgattt ctggatgcag cacagtctgc aacgggccaa aaaattcggg
    14761 aacaacgttt gcgaaggacg cgctcgcgcc tttcgttgaa aaaggagagc ttcccggtgc
    14821 gatcaacgtc ttttacaagg acggcctgca agaggtcgcc tgcgtcggtt atgccgacgt
    14881 cgcggcgaag cgtccgatca cgatggacga cgttttcatg cagtgctctc agacaaaggg
    14941 tttttgcggc gtgacgatcg cgatccttgt tgaggagggg aaaatcacgc ttgacgaccc
    15001 ggtttccaag tatctgccgg aattcaaaac gctctgggtc catgaatcca ccacaaacgg
    15061 cgtgaagcgg cttgtccagg cgaaaaaccc gctcacgatc cgcatggtca tgaaccacac
    15121 cggcggcttc ccgttcgaga tttgcgcgaa gcagggtaac atcaagggcg gcggctggtc
    15181 cggcggcgcg ccgttgcggc agaccgcggc gatcgcggcg gcgtgtccgc tgctcttcga
    15241 gcccggcacg aaagttcagt attccaatac cggcatcgac atcggcgcgg cgattattga
    15301 ggtggtgacc ggaacacgct gggaggattt cctcaaggag cgtgtctttg atccgctcgg
    15361 tatggcgtcc agttcgttca agccgaccga caagcagctt aagacgcagg tggagatgta
    15421 cgactgcgtg aaggacgcac ccgcgaaaca ccgcctgcag aacgagatgc agcaacgccc
    15481 ctacaacggc gatcacgtct tcgcgtcggc gggcgcgggg ctctggacga ccgcgagcga
    15541 ccagctcaag ttctacaaga tgctcatgaa tctcggcgtg ggcgagaacg gtgtgcggat
    15601 cctcaaagag gagaccgtga aggagatcct cgcgaaatcg acacgcccga agggcatggg
    15661 cgggtattcg ctcggactca ccgctccgga ggaggacaag gaagactcct ggttcgggca
    15721 tggcggcgcg tggggcacga actgcatggt caactggcac cgcagggagt tgaagctttg
    15781 ggccgtacaa ctctgcggca gtccgcgccc ttgggacaag gctcgcacgg cggcggcgga
    15841 taagttcttc cagtacgcga tcgacaactc cagtgctgac gcctacaccg gccgcgttca
    15901 gtaaacctaa aaagagcagt tgaacgttca acgctctttg gtttcagggt ttctgggttg
    15961 agggtttcag ggtttcaggg tttctgggtt gagggtttca gggtttcatc taaatcgggg
    16021 tcgggatcgc tatcgggatc gggatcggcg acccgcgaca ttcgacccgc gacgatcgac
    16081 agggacgccc gcgcttccga caccgcaggt cgctcgtcgc gggtcgacat ataaggtctg
    16141 acgggagaag cgggcgtccc gcccgctggt agggacggtt ccctgaaccg tccgcggacg
    16201 cctcggggag gcgtccctac cgattgattg gcggacgcct tagttccgca tatgcaccgg
    16261 tctccaaaac tcagccgatt gcgcttgcga cgaagggctt tgctaaaatt ctacccgttc
    16321 aactgctcct ttttgggttc aacgctcttg ggtttccggt tcctgtagac agaagaccgt
    16381 aggcgggaga cgggaaacaa gcgtcggcaa cgcggacgtc cccgccctca ggggcaagca
    16441 gacgcttgcc ccttgttttc atttcatgac gtttcatgtg tttcatggtt cgagtgttga
    16501 aacccgaaac ccgaaaccca aaacccaatt agctggggat tgactgcgtt cacatccctt
    16561 tcgtatacta tacgcaatgc tgattatgct gtgattgatc aagcagaggt gtggtttccg
    16621 aacgcaatca gaaggggagg ggaacgtatg tgtaaaagat tatgccgcat ggtggtcgct
    16681 ctttcgatga tcacggttgt ccgtgccgat cagcacaagt ggaaatccgc agtgtccggt
    16741 aactggagcg atctttcgaa atggagtatt gcaagcgctt tgccggaagt gacagacacg
    16801 atccagtatg gcgcggtcgg cacgttcacc atcacgaacg atacgggcgg cgacatcacg
    16861 ctggtgaaaa tcgaccagtc ggcggtgccg ggtgccacga agttttgcgt catgacgctc
    16921 gacatgcagg gacgcacgct gacgctcacc gtgtccgaca acagctccat tttctcttct
    16981 ggatattacc aatataaaaa cacgcgggac ctcgatcccc cgacgtgcac ctacgaattg
    17041 cgcaacggca cattcgattt cacggccggg cgcctgtttc tcggcaacaa cgggatggag
    17101 gccgacggcg gcatcatcct ctcgggcggc gccacgctcc acgcgtatac gtaccactgg
    17161 aacgactctt cccgcgtgtt catccgggag ggcagcgttt ggaactggag caagcccacc
    17221 gctgacctct ctatgcagag cgggggatgg caccgtggct tcgggttcct ctgcgtgacc
    17281 ggggaaaact cccgcgtgga gatgaatgga cggtcggtct cgatcaacaa cgacgattca
    17341 ggggtctatc tgttcgacgg cggcgccctg aactgccaga ccctcacggt cggcaccggt
    17401 tcgcacggat cgaacggttt cgttcaggtc cggaacggca tgctctgtgc ccagagtttc
    17461 aatctcggca gcacggccct gacggcgtac aagccgactc ttaccctcgc gggtgagaca
    17521 acgcttgttg ccgtgacgaa cacgggagcg acagccaact tcacctgcta cgagcgcacc
    17581 ggcgcgacga tcgccttcga aatcccggcg gacggttacc gcacgaacgg cgttgcccgc
    17641 gcaccgctcc tgctcgacaa cctcgccttc aacacgacac gcagcacgtc ctatccgaac
    17701 gatggcccga cttcgctccg gatcgcctgt atggcgtggg ccgtcgcgca cccgaacgcg
    17761 acgatcccgc tcgtttccat ccgtaaagcg ggcggcgaca aagcggatct tgaggccctc
    17821 acgaacggct tcgtgttcgt cgatgtgccg acctcggtca tcgaggccgg aaacgcgccg
    17881 gtcctttcga tcgacgaggg cggcacgaca ctttttatca ccgcgccgga gtcgatgggt
    17941 acaaacccga cgcccccgga gttcacggtg gaatctgtca gtagcggtgc gaacggcatc
    18001 cgcgacatct tcctgacaat cagcacgtac ggctttctct cgactcaggc gacccttgtg
    18061 gaactcacgg ttgcgacgaa tgaaaccttc tcgggggccg tcgtgacgaa ccttgccacc
    18121 caaatcgagg cgcccacgcc ctattcggcc gcgttcaagc ttcccggttg cttgcagcgc
    18181 cagcactact ggtgccgcct ccgggtggtg aacgacaacg acctcgacgc gacggtgccg
    18241 ttcgaattca cggacgaggg atgggaggag acctacgtgt gggcaaacgc cgcagacggg
    18301 cactgggaca cgctcgcgaa ctggatgacc ggtggcgcgg cggctacgga gttgccgcac
    18361 ggggaagaca tgctcagttc gttcccggtc ggctcctata cggtcacgct cgattcggat
    18421 tccgaaatcc gttcgatcgg attcttcacg gcagggacgc tcgaagtgcc gcgggtctac
    18481 acgtttgacc tgaacggcaa gacgctgagg acgacgaaag tgacgcagtc ggtgacgggg
    18541 ttctccggaa cctgtccata ttcccctgcg gtgccgatgt ccggacagct cgttttcaaa
    18601 aacggcacgt tcgatgcgtc cctttcgacg agcttgtcga cgttcgagga tgccgaactc
    18661 cgggtgacgg acggcgccac cgtatacctc aagctggtca tgaacggcgc ggcccgcctg
    18721 attgtggaca acggcggctt cttccggccg caggaccaca tcaccctgaa cgcgaacaac
    18781 aggacatata agacatacgg tttgatccgc gtcaacggcg caggctcgaa atacggaagc
    18841 tgggctgatc tgaacgtcgg cggcacgctt tcagccgtga tcgttgaaaa cagtggcctg
    18901 atcacggtag gcagcctgaa catcggcggc aaccgcaata cgcccactgc accctgttcc
    18961 aactgcgtcg cgcgtgcgga caacggcacg ttcgagcttt ccagctcgct caacctcgga
    19021 acggccaacg ccgaatgtta caggccggag ctgcgtatca ccggcaccaa cggctttgtg
    19081 cgggcgaccg gatcaggcaa gatctcctct ttttacgaga acatcggcgc aacgatcgct
    19141 ctggagatcc ctgctgaggg gtttgcggat accaacggca cggcacgcgc gccgatccac
    19201 ctgaacgcgc tccagttcgt ggcccgcggg gcgggactcg cggacttcgg cgcgacgacg
    19261 cttgaaatca ccgcccggga ttgggtgaag gcgaatccga aacaaacgat caaactggtc
    19321 gaactgatca cccccaacgc ggaggcgctc accaagctga aaaacgcggc ggtctttacg
    19381 gatacgagcg acgcgacctc tccgacgctc acggtctccg aggacggcaa ggtgctctac
    19441 gccaccgcgc cggtctggcc gggtttcatc atgctcttct actgacgtgc acgccgcccg
    19501 cggtttggga aggcgacggg ggcggtcttt gtgatgtgcc ggtggcacac gccgcgtcct
    19561 tccgctgacg gttttcggtg cggggtgtca acatgtgtac gggcgtgccg ccggggtgtc
    19621 ccgtgacggg atgacgtttt ttttggtttg aaccgcaata tcgtgtccgc tgatccgtta
    19681 gcatgaacca tgttgactgc atggcaccgg caagcagaga atggggagag ggaatgacga
    19741 ttcggcagaa agtggcgttc ggattgggat gcatcggatg cgcgatggca ggcgctggaa
    19801 tcgaatccgc acggcgggcg ctcaaagtgc cttcggttcc cgacatgatg caggtggagc
    19861 gggaagttgt tcggctaacc cgcactccgg ggcagacggt ggtggtgcgg gatgaggcgc
    19921 tggccagtga ggctgaagcg ctgcgaactc gggttgccga gttggagcag gcattggccg
    19981 cacgggatgc ggcaccggcc gctgtcgcgg tgtcgcatct tgccacgaat gccgcaccgg
    20041 cacgtgccgg tcggccggaa agggagtcgt gggagcagcg gatggagcgg atgcggactg
    20101 aggagccgga gcggtatgcg gaaatgcaga agcggcgcga ggagttccgc cagcagatgg
    20161 aacagcgcgc ccgcgagcgt gcggactttc tggatgcggt tgacgtggcg ggtatgaatg
    20221 acgcccaacg ggagaatcat gagaaattgt tggcgctggt cgcccgcgcc aatgagttgg
    20281 gcatgcagct gatgcagggc ggcggacgcg gtgagcaggg cgaggcgttg cggagagaga
    20341 tgggggagac catgtcggcc ctctcgacgc tgtatgagat ggagcgtgat tatctgctgg
    20401 aggccacggc caaagcggtg gggtacgatg gggaggatgt gaaggcgttc accgaacaca
    20461 tccggcacat catggagaac acgtccccga tgccgggctt cggacgacgt cgcggaggca
    20521 ggtgacgggg gagcgcaacg cgcccgctca aacgctgaac aggaagtgca tgacgtcgcc
    20581 gtcctgcacg gcatacgttt tgccttccac ccgcatgacg cccgcggctt tggcgccggc
    20641 ctccccgttc aggcgcacgt aatcttcaaa cgcgaccact tcggcgcgga tgaacccgcg
    20701 ttcgaaatcg gtgtggatga cccccgcgca cgttggtgcc gcccaccccc gtcggaacgt
    20761 ccaggcccga acctctttgg ggccggccgt gaaataactg gccaatccga gcgtgtgata
    20821 agccatacgg attgtctgat cgaggccgct ctcggtcagg ccgtatgagg cgagaaacgc
    20881 ctcgcggtct tcaacgggaa ggctggccag ttccgcctcc attttggccg agatgaccac
    20941 gacatcgctg cctttttctg cggcgaacgc ccggacgcgg gcgaccgatc cgctgtttgc
    21001 cgcgaggtcg atctctccca cattggcgca gtagatcacc ggtttgtcgg tgatgagctg
    21061 aagttcggtc agcgtatttg cgatcggatc cgcctcttcc cgcggaaaag tccgcaccgg
    21121 gtttccagca tccaaatggc gcagaagacg ctccatggcc tcaacttctt tgcgcagatc
    21181 cttgtcggcg cgcgcttgcc gcgcgatacg gtccagccgt ttttcgaccg actgcaagtc
    21241 ggcgaggacc aactcggtct ccacgatttc gatatcgcgc acaggatcga cttcaccctc
    21301 gacatgcacc acatcgtcgt cggcaaagca gcgcacgact tgcaggatcg cgtcggtttc
    21361 ccggatgttc gctaaaaact tgttgcccaa gccttcccct ttgctggcgc cgcgcaccag
    21421 accggcgata tcaataaaat ccacggtggc tttgacgacc tgctccggat ggacgatctg
    21481 cgcgagcgca tccagccggg ggtccgcaag cgcgaccgtc gccttgttgg gttcgatcgt
    21541 gcagaaggga tagttggctg cctccgcatt ctgggcgtgg gtcagagcat taaacagggt
    21601 agacttcccg acattgggaa ggccgacgat accgactccg agtgacatgc ggaagattcc
    21661 ttttcccgaa ccggttgtcc ggcgatcctt gctctggcct gtacgacata ggctgtgttg
    21721 gcctgatcgg acatgatacg catggtgcca ggagagggag tcgaaccctc acaccctttc
    21781 gggcggcaga ttttgagtct gctgcgtctg ccatttcgcc atcctggcgg ggatggagcg
    21841 ggcgaaggga atcgaaccct cgtaaccagc ttggaaggct ggggctctgc cattgagcta
    21901 cgcccgcaaa aatgttgtgt tactttaccg atttccgttt gtgcggtcaa tagcgcggct
    21961 aatttattca cagtcgaatg gtttacggca catgcggcgg cttgccttca accacggaga
    22021 ttgctactat aacagggttt tatggtgttc caagtctggg gcactgcggg aaagacaaga
    22081 ggggcgcaag acgcctgccg catgtactga aaacccttta acacggagat gagtatggag
    22141 ttgcgcaaag cgtgcaagta tgcgttggcg gtgccgacca gcatgggcat tcggatcacg
    22201 ccttcgaacg gccagccggt gcattgcggc ggccagttca tgatgcaggt gaccagtgct
    22261 gagacaaatg tggccagcgt ggcagcctat ctggggttgc ctgtcaaggt cctgaccacg
    22321 tttgtgaaag acagtccgat tgcgcggctc atccaggaca acctgcgcag tcggcacatg
    22381 gattacgagg gggtcgaggt gccgcagggc ggaccttggg gttaccgtca ccagatcaac
    22441 atcgcggaca gcgggttcgg ttcgcgcggg ccgcgcgtgt ggaatgaccg tgcgggcgag
    22501 gtgggccgca cgctgaacgt gaaggatttc gatctggagc ggcttttcgg tgacgagggc
    22561 gtgcagatcg tgcatctttc cggcctgatc ggtgcgctgt cgcctgaaac cgggaccttc
    22621 tgtctcgaac tcgcgcgcgc cgcgaaaaaa cacggtacgc gaatctcgtt tgatctgaac
    22681 caccgcgcct ctttctggaa ggggcgcgag tccgaattgt cggcgatctt tgcggagatc
    22741 gcgagcatcg cggatattct ggtcgggaac gaagaggatt tccaactctg cctcggtgtg
    22801 cgcggaccgg aggcgggcgg caaggacatc aaggctaaga tcgacgggtt caaagggatg
    22861 atcgaacgcg tccggaaggc ctatccgcat gcgcagacct acggcacgac gttgcgcgag
    22921 gtggtggaca ccaatcgcca tttgtggggc gccctgatgt gcgcgggcga cgcttggcat
    22981 gtggtcgagc cgcgtgacat cacggtgctg gaccgtatcg gcggcggcga cgggttcgtg
    23041 ggcggcatgt tgtacgcgat tctgaaaggg tgggagcctt cgaagtggat ccagttcgca
    23101 tgggcctgcg gcgccttggc cacgaccatg ctggtcgatt acgcgctgcc tgccgatgag
    23161 gagcaggtct ggagcgtctg ggaaggcaac gcccgggtcc aacggtagcg cgcgccgggc
    23221 atcgaacgcc ggacgataaa ggagcgggca tgctcttcgg gcatgcccgc ttcaccgggg
    23281 aagaccccgc ttggcgcgct gtccgtccgg tatgtcgcgc gcaaagaaac cgtcgcggaa
    23341 gaaaacctgc gcaaaggcgc ttacagcccc atgtcctcgt tgaccaccag aacatggaag
    23401 tcggtcaggg tcggcttgct gtcgaggatg gtggtgacgt gacagatgcg ctggggagaa
    23461 tcgcaatcgc tgcaaaagcc ggtcgccgcg cagggggttt gtctcccgag gcgcatggcg
    23521 ttcggcggcg cggacttgtc cttgatacgc cggatggcgt cactcacatc gccctccaca
    23581 attttgttgc gtcccacgac cacgatgact ttggtcgggc cgtaaatcat agccgcgacc
    23641 cggttgccgt ttccgtcgat attcacgatg cagccgtcga gcgtgaccgc gttcgcgccg
    23701 gagaggaaca ggtcacacgt ctgttggcgt ttcatgatct cgacgcgctg ttccggagtg
    23761 agatccggga acccgtggtt caggatctcc ttgccttgag cggtgaggtc ggcggtcagt
    23821 tcgaggtcag ccacggagag tgatccgccg aatccgacgg tgtgcgcgtc tgcgctctgc
    23881 ttcagaatat actctgcggc ctcacgggcg caggcgcaat atacggcggt gaagtgacgg
    23941 cgggcaaggt tctcgaccaa tttgcggcac tttttttccg aggtccagag attcagttca
    24001 cgggtacggt ctgacatagg tattccttat ttggttttgc gaaacaagac aatcatccgc
    24061 tgcgggacgc ctctgcggat cgtccctgaa acagcggtat tccacatcca aaaacatcat
    24121 gtggaatttt acatgatccc tcatgggatt acaatcctca aagcggtttt cccgcaacgc
    24181 gcggatcagt cgaatcgggg tgtccttaca agagaacgta aacgtcacag gtcgaatgcc
    24241 gcggggcgca ggtcatgttc acgggaatgg ggacggggaa gaggacgagg acgtgtgcgg
    24301 ggaacgcgtg cccgcatctc cttgacaacc gtggggcgtc ccgtaagata catgttcacc
    24361 gggatcgggg agtgtccggt tttcggtgaa aggggcggac gggaggtatg cgcggacgga
    24421 tcgggaacgt ggcggcgggg gtcttgttgg cggcagggct ggcgcaggcg ggaccaacgg
    24481 tgttccggcg cgggatggac cgggtctcga gtctggatcc ggccatggcc tcatcgctgt
    24541 atgcggcgcg cgcggtgcag ttggtgtacg agacgttgtt ggagtacgac tatcgcgcgc
    24601 gtccgtaccg cctcattccg gggttggcga cgcatttgcc ggaggtgcaa tccaacgggt
    24661 gcgtgtatgt gttcacgctt catcctgagg cgcgttttca ggcggatccc tgtttcggtc
    24721 cggacggctc cggtccgccg cgcggccgcc cggtgacggc ggaggatgtg gtgttttcgc
    24781 tcaaacgtct cgcggaccgg cgtctcgcct cgccgggttc ctggctggtg gaggatgtga
    24841 tcgcggggat gcgcgccttt gccgagacct ccgagacggg atcggccacg gattacggac
    24901 ggccggtcag cgggttgcgg gcggtggatg agcgcaccgt gcggattgaa ctcacgaggc
    24961 cgatgcacca gttcatctgg tatctggcga tggcttatac tgcggtggtg ccgcacgagg
    25021 cggtccgttg ctacggcacc gatttcggta gccgggccgt gggtagcgga ccgtatcggc
    25081 tgacagactg gaaacgcaat caccagatga gctatacgcg tgatccggag tggcgcggct
    25141 ggcgcagcgg tgccgcagcc atcgacgctt cggacggcac ggtgccgttt gaccgtgtgg
    25201 agtaccgggt cattgacgac gtttccacgc agtggctctg ttttttggcg ggcgagctgg
    25261 atttcttagg tgaggtgtcg cgcgacaact gggacgtggt ggtggatgcg gcaggcggtt
    25321 tgacagagcc gttacggcgg cgcggtgtga ccctcttcgc catgccgacg ctggaggtgg
    25381 cttatgtcgg gatcaacatg gaagatcccg tgctggggaa aaaccgcgcg ttgcgtcagg
    25441 cgttgaactg cgcattcgac cgcgcggcgt gggtgcggtt tttcaataac cgggccgtgc
    25501 cgtgcgacgg gcccgtgccg ccgggcacgg aagggagact ggagacgccg tttccgtacg
    25561 cctacgattt ggacaaggcc cgagcgctgt tgcgcgaggc agggtatccc gacggcatgg
    25621 atccccggac cgggcgacgc ctggagctga atctcgattt gggccgcacc tcgcaggata
    25681 tccgcgagtc caccgaactg atggtttcct ttctggccca ggtgggcatt gccctgaagc
    25741 cgcaatatca caactggccc gcttttttgc gccggttgtc ggagcgccgg tcgcagatgt
    25801 tccggatcgg ctgggtgggg gactatcccg atgccgaaaa ttttcttcag cttttttaca
    25861 gccggaatgt gtcgccgggc cccaatcgga cgaattacgt caaccccgag ttcgaccggc
    25921 tgtacgaggc ggcttgcgcg gcggaggatg cggctggccg caaccgtcat tgggctgcgg
    25981 cgcaaaggct gatccgggag gattgccctt ggatttttct gagctatgcg aaagcctaca
    26041 gcctttgcaa cccgagtgtg ctatactata tgccatctga tttcccgtac ggcaccgaga
    26101 aatatctccg cacgcgcgca gaacgggagg ggatctgaag gaggggttct tgaccgccat
    26161 catacgatca tgcggaaagc cggaaatggt tcggtactgc tatcgcggga ccgcgcaaat
    26221 cgggcggatc ggcgttgtgg gaacccataa agcggtgacc cgcctgcttt ttgaggaaga
    26281 cacgccgcca gccgacgcgg aaccgggcga gtgtcctttg ttgcgggagg cgttgcgcca
    26341 gatcaccgca tatctcgaag gccggttacg gtgcttcgat ttgccgttgg cgccggaggg
    26401 cacgcccttc atgcgccgcg tctggaagcg ggtgctggag gttccgtatg gcactacagc
    26461 ctcgtatggg cagatcgccc gggcgatcgg ccgtcccgga gcggcgcgtg ccgtggggat
    26521 ggccaatggc gccaatccga ttccgatcct gattccctgt caccggatca tcggtgccga
    26581 cggtacgctg atcggatacg gcgggggatt atcgcggaag gcccgtctgt tggagatcga
    26641 gaggagtctt ttttgaaaga caatgccaat aaaacggtag cggcaccgtt ttcttctgtt
    26701 gtaaacccca tgcagccgga gaccgggacc gcagtagaaa gcgacgccag tttgttgagc
    26761 gcctttgccg agggtgatgc ggcggccttg gacaggttgg tggggcggta ccggtcggcg
    26821 ctgttttcgt ggttgctggg catgaccgcg aatcgcgccg acgccgagga tctctatcag
    26881 gaggtttggg tgcggatcat ccgccatgcg gcgcggttcc atgacgtgtc cttcagggcc
    26941 tggatgtgga agatcgcgcg gaatctgctg atcgatttcc gacgcaagcg aaaaccggtc
    27001 gtgagtctgg acgtggtttc ggatgaggag gaccggccgc tgatcgaaca gttggccgac
    27061 accacagccg gaccggtcga gagggtggag cgcggggact tgaaggcgtg tgtgatgcgc
    27121 gcggtcaagt tgttgcccga ggtgcagcgg gaggtgttcc tgatgcgggt gcagggcgat
    27181 ctgccttttc atgagattgc gaagacgctg gacattccgc tcaatacagc actcgggcgg
    27241 atgcacgacg ccatgaccaa actgaaacgg atgttggccg aggagaattt gagatgaagc
    27301 cgcaaaacac attcgacgag attgccgcgc tggagcgtga tgcagcggcg ctcgcaccgc
    27361 aggttcgcga ggcattccgt tgggcggggg ggccctccgc aacagtagag gaggccattc
    27421 gtcaagaggc ggctcgcgtg gccgcgcgtg cccgggtgcg atggcctgta tggtcactgc
    27481 gccggttgca agtcgtcgcc gttgcatgtc tggtgctgct gctggctgcc ggcggtcttt
    27541 tgttgacttc tccgcaggtc taccgggcga aggctgtgcg tcagacggcg catgtgggca
    27601 cgcggcctgc gggcggcgcc tcggaggatg cggcggggtt cgcccggctc ctgctgaaca
    27661 ttcaggggtt ggacgaggat tcgttcgtca cggatggggc atcggaagct ctttggctgt
    27721 agaccagagc ttttcgatgg cgtaatacgc ccgcgcctca ggcgaaaaga cgtgcaccac
    27781 gacgtggata taatccacca ccacccagcc gctgtcgggt tcgccggatt tgcggtaggc
    27841 agtgaccccc aacgacttca tacggtgctg ggtttcggcg atcagtgctt tgagatgcgg
    27901 ggccgaggtg ccggttgcca ccacataaaa atcggtcacg gaggacatgc cgcgcacatc
    27961 gaagatgcgg atcgcgtccg cttttttgga ctcgagtgcc gatgctgttt cgagcgcaat
    28021 cttttccgag ttaatggtca catctccttt actgtccggt aaagtccgcg ggtgcggata
    28081 taagcttcga cctcatgcca aacaagatac cggattgttt tgccttccgc aattcttctt
    28141 cggatctccg aggcggaaac atcgaaaagc ggtccccgga tcacgttcgc cagcagccgg
    28201 tcccgcacct cttgcggaaa ggagagggct tccccgggga gttcccgcac atccatgccc
    28261 ggccgttcga cggtgatgac ctcgcacagc ttgagcagcg catcggcctg atgccagtgg
    28321 ctcaatccgc acagggtgtc catgccgatg atgaaggcga agcggtcatt cggatactgc
    28381 gcacgcagcg cgcgcacggt gtcgatcgta taggagacgc ccccccgatc cagatcgagt
    28441 gtgctgactt ggaacagcgg atcgccctgg atgctcagcc gcaccatctc cagacggtcg
    28501 gcgtcggggg cgaggtccgc cgcgccgatt ttaaagggcg acacgcaaca cggcagcaac
    28561 agcacgcggt cgagcccgaa ggcctctgcg gccgcgcggc cgatgagcag atgccccaga
    28621 tggatcgggt tgaacgtgcc gcctagaatg ccgatacatg ccatgaaaac ctcccggatg
    28681 cgttcagccg atcacgtgtt ccgtttcggc gtcgaaaaac agcgcgtgcc gcatgtcgag
    28741 cggaaaatcc gccaccgtgc ccggtgtcag gacacggtcc ggatggacac gcgcgatcag
    28801 gcgcgtcccg ccttcggcca caagctggac aaatgtttcg gcccccatgg gctccacacc
    28861 gtccaccgtg ccgcgcagcg atacgccccc ggccgcgtca tcggccccca gggcttccgg
    28921 ccggaaaccc agcagcacct cgcggtctgc ggcctgcgcc agcgacgaca tggcgacagg
    28981 cggcaacggc aaccgggcat ccggcgcata ctgaaacagg agcgtgtcgt ctggttgtcg
    29041 gaccagtctt ccgcgcaaca cattcatggg gggcgtgccg atgaatcccg ccacgaaccg
    29101 gttggccggg cggcggtaga cctccatcgg gctggcggcc tgctggatgg tgccgcggcg
    29161 catcaccacg atccggtctc ccatggtcat ggcttccgtc tgatcatgcg tcacatacac
    29221 catggtcgcc gcgagacggc gatgcaggcg ccgtaactcg ccgcgcatct ccacacgcat
    29281 tttggcatcc agattcgaga gcggctcatc gaacaggaag accttgggtt ggcgcacgat
    29341 ggcgcggccc accgccaccc gctggcgctg tccgccggag agttgtttgg gcagccgccg
    29401 caacaggccg tccagtccca gaatgccggc tgcctcctgt acgcgcgccg tgatctcggc
    29461 acgcgggaac ttgcgcaacc gcaggccgaa tgccatgttt tcgaaaacgg tcatgtgcgg
    29521 atagagcgcg tagttctgaa acaccatggc cacgtcacgg tccttgggcg gcacggcgtt
    29581 cacgcagcgg tcaccgatgt ggatcgttcc ggacgtgatc tcctccagac ccgcaatcat
    29641 ccgcagggtg gtggacttgc cgcatcccga cggccccacg agcaccagaa actcaccgtc
    29701 gcggatggtg agcgtgaacc ggtctacggc caatccgccg tcatcgtatc gcttgcagac
    29761 cttgttcagt gtgacttcag ccatgtctct tccgtggggc ctccctacag caccaggcgc
    29821 acgccgggca cctgtttgat ctccgcgtcc agacgcaagt gttcttcccg gtcttcgagc
    29881 ctcaccgaaa cgcagaatgc gcggtaacgt cccgttgacg agtcccgcga gacagcgagc
    29941 ggcgaggtga cccggtaggc ggcgatccgc gcggccagcg cggtttcagc atgcgtcgcg
    30001 gtttccgtga gaatccggaa gtggaactcc ccggggtagg caggctcttg cgccgcgggt
    30061 gcggcgtcgc ggttttccat acggggggcg gggcgacacg aattcatgag actccttttg
    30121 ccggggttgt ggtctggata ctcgccgacg cgagcaactc gcgcgcatgg ctctcgatca
    30181 cgctggtcaa tcccttgccg cccagcatgc gcgcgatctc atccacgcgg ccggcatcgt
    30241 ccagagtcgt gatgcgcatc cgggttcggc ctcggaccac ctcttttgtc accaccacgt
    30301 gacgatgccc ataaacggca gattgcggca gatgggtgat ggagatcacc tgatgggttt
    30361 ctgccacgcg gcgcattttt tgccccacca ctctgcctat ctcgccaccg atgttggcat
    30421 caatctcgtc aaacaccagc acggggatcc ggtcgtgcaa cgcgagcacc gctttcaccg
    30481 cgagcatgac ccgcgcgatc tcgccgctgg aggcgatggc tttgaggggc cgcatcggct
    30541 cgcccgggtt cggcgcgaac ccgaaggaaa ccgtgtccgc gccgttcggt cccggcgggc
    30601 agggactcag ttctacgtcg aaccctgatt tcaggaaacc caggtcgtgc agttcttgcg
    30661 tgatcgagcg ggcgagtgcg ccggccgcgc gcgcgcggac ggtcgaaagt gcggcggccc
    30721 gcctcgtcag ggtttgggtt gcgtccgcga tctccttgcg gagcgcatcg gcctgttcgg
    30781 tgcgggtggc gagatcctgc aaccgcgctt cgctcttttc cagggtggcc aagacctcgc
    30841 tcactgaaga accgtacttg cgtttcagcc gctgcaccag tgccatacgc tcttcaagat
    30901 ggctcaacct ttccggatca gcctcgatcc ggctcaaaca atccgtcacg gcccggccga
    30961 gttcctgaat ctgcacggcc gcattccgcg cttcggcgag ccagtcggcc gcttccggca
    31021 gaagccgcgc cagttccgtc agctgcgtct gaaccgcgac cagtccgctg aacacggctg
    31081 gttcgccatc ggtgaggcct tccaccacgg cgtttccgag tgcgagaatc tgttcggcat
    31141 gcgcggcttg ggcgtgtcgc gaaaccaggt cctcttcgtc cgcctccgtg agctgggcgt
    31201 gggtgatctc ttccgcgatg aatcgcagtc tttcggtttc cgcctcgatg ttcggagcgt
    31261 cgcgttgcag ttcgtcgaga cgcgcctcca acgtctgaag ggtttgccag acctcggcac
    31321 aggcttcgcg ttgcggcaca cagtgcccgt acgcatccaa cacatcccgt tgaaaggcgg
    31381 agtccaacag cgactggtgg tcatgagggc cgtggatgtc caccagcaga ttccccagcc
    31441 tgcggagcgt ctgggcggtg gcgggcgaat cgttcacaag acagcgtccg gcacctgaag
    31501 cagaaaccga acggcgcacg agcagcgcgc cctcttcaca gggcggcagc cccgccgact
    31561 ccaaaagcgc atccacctca cgcgtatcgg caagcgcgaa ccccgcctct acccaggctt
    31621 ccgccgcgcc ttcgcggatg gccgacttgt cggcgcgtcc ccccaagacc agatcgagcg
    31681 cacccatgag cacggatttg cccgcgccgg tttcgccggt gatgatgttg aggccggggc
    31741 cgaaggtgac ggcagcctct tcaacgatcg cgagattttt gactgacagg tggttgagca
    31801 cgacttttta tcacattcaa cggattcagg atcagacaag ggaagtgtaa cacatccgcc
    31861 gctacgccgt aaacggtatg aaaaagccgg gcaaaaatac gaagggacag ctggagcgct
    31921 gaatgtcgaa ttgcgacgct ttatcaggtc gaggatgcgc cccggttacc ctgtccagcg
    31981 taagcgggct tgcatccgga gtgttcaaac gccataatct gttctcacgt tttgatgtaa
    32041 aaggagagtg aagcatgcgg aagaagattg ttgcaggaaa ctggaagatg aacaagacgg
    32101 cttcggaggc cgtggccctg atcgaggaga tccgaaaaga aaccgaaggc gtttctgcgg
    32161 tggagatcgt ggtgtgcccg ccgttcacga atttgaagga tgcggcggca gcctgcgccg
    32221 gaacccatgt gatgctgggt gcgcagaatg tgcattggga ggcttccggt gccttcacag
    32281 gcgaaatttc aggggcgatg ctcaaggacc tgggcgtgca gtatgtgatc attggccaca
    32341 gcgagcgacg ccagtatttc ggcgagaccg acgagacggt caaccgccgt gtccgggcgg
    32401 cattggctgt ggggctgatc ccgatcgtct gcgtgggcga gactctggcc gagcgcgatg
    32461 ccggacagat ggagtccgtg gtggtgcgcc agaccaagga ggggttggcg gacctcggtg
    32521 aggatctggg acgcgtggtg atcgcctatg aacccgtttg ggcgatcggt accgggcgga
    32581 cagcctcgcc ggcccaagcc caggaggttc acgcgctgat ccgccgcaca ctggccgaga
    32641 tcgccggagg cgatgtggca aacacgatcc ggatccagta tggcggcagc atgaagcccg
    32701 agaacgccag agaactgatg agccagcccg atatcgacgg cgggctgatc ggcggcgcgg
    32761 cgcttaaggc cgactccttc gcggcaatca tccgggccgg catgtaaggg gtttgcctta
    32821 gcggatctgg gcgacccaaa gagacggggt gctttggcga ggcgcccctc ggttccggtt
    32881 tatcgaaatc gttgtcgggg tcggcgtctc gtcctcgttc atgtgaacgt cggcttatga
    32941 cctgtgtgtg accttcgccc cgcgacgttt cagattttgg aatccgttta ggacatgatt
    33001 tccgacagct tcgcttcggc ggggagaaat ccatgattac catgatgttg ttcaatcctg
    33061 aaaatcctgt catcctgtca atttataggc aagagttgac cgttccggac cgtccgcagc
    33121 gtgaaacccg agccccaaaa ccaaagagcg ctttgaaggt tcagctattc cagggttcca
    33181 atcgggttgc cggattccta taatgggcgc tcatggagag ggaaacccat cttgttgagg
    33241 ggagtgccgc gcagatgcgc gtgccgcagg aggctttgcg gcggcgtgcg ctggaagagg
    33301 gcgttgcggc gtatgtggcg cgcgagcgtc tcgttccgcc tctgactgcc gccgaaatgc
    33361 tggcgcatgc ccgtgcggcg accgcttcgg aaaatctgga tgatgcggag gcggtgttcg
    33421 cggcggttct gctcaacaat gcggtttggg cggatgtggt cgccggtatt ccctatgagc
    33481 ggcggttgtt gctccttccc ctttgtttgg cgcggaacga ggtgtgccgg gcggagcgcg
    33541 acggtttggg tctcttgtgc gcgcaatgcg gcaactgtcc gatcggcctg atccaggcgg
    33601 aggcggaccg gctcggatat gtctcgttgg ttgcggaggg caccacggtg gtgacccagt
    33661 tgctcgccgg cggcaaggtg gacgcggtga tcggtgtggg ctgcatggat tcgctgcgcc
    33721 gtatgttccc cattatgaac gcgcacgcga ttccgggtca ggggatcccg ctgctgacgg
    33781 atggctgtca ggccacggag gcggatgtcg catggatcct gcgtgttttg cgcgcgagtc
    33841 gtcagggggg cgtgtcgggg gtcacggaga tcgaggcggt ggccgatgcg gttcgcaact
    33901 ggttcacgtg cgaggagttg gccgccgtgc tgggctcggc ggagacggaa gcggagcgga
    33961 tcggccggga gtggctgctg atcggaggca agcgttggcg gccgctgttg accgccgcgg
    34021 tttcggaggc gctggggggg gcgcgtgacg ccatccgtcc tgcggcgctg gcggtcgagt
    34081 gtttccacaa ggcgtccctc attcatgacg atatcgagga tcaggacacg gagcggtatg
    34141 gggtgccgac cgtgcattgc cggtacgggg ttccggccgc gatcaatgcc ggagattatc
    34201 tgatcggtga aggctaccgc ctgctggccg aggccgcgtt cccggatgac ctgcgcgcgc
    34261 gtatggtgac ggcagcggcc cgcggacacc gcgaactttg tctcggccaa ggtgaggagc
    34321 tggcgttcag ccgccagccc gcgccggtga gcgagtccgc tgtgctgcgt atctatgagc
    34381 gcaaaaccgc tgcggcgttc gaggtggcgg ttttgctggg cgcgatcgca gccggagccg
    34441 atgcggacac gtgcgcgcgg ctttccgaat tcagccgtgc ggtgggtacg gcgtatcaga
    34501 tcggcgatga catggaggat ttcttctcgg cgcgcggtcg ggccgcggac ctgctcgcca
    34561 tgcgtcccac ggtgtttctg gccacagcct gcacatgtga caacgcggaa gtcgccgcag
    34621 cgctggattc ggtctggaat gcggaccgcg ccggacggca gcggctgatc gacgcgatca
    34681 cgcaggccgg cctgcatacg cgggtccgcc tgttgtatga gcattatcgg catgagacgg
    34741 aacggaccat cgccggaatt cagcataccg gcctgcaacg cctgttgcgg catgtcatgg
    34801 ggcggatgct gcgataggcg ccaggcggcg cggaccggac ggactgatga ggatacggca
    34861 agccatacct ttttgcgtga cctgcttggc ggcggtgtgc gcggtcggga cccttgcggc
    34921 atggctcgcg cgcgaatccg ccgtggacgc gtccttacgt ctggcgaccg ccgacggctc
    34981 gggtgccgag gcggcggcaa tcacggaatc agacgcgcgc ggaacggatc tgaccggttg
    35041 tttccgcgcc aaccgggaga tcccccgggg agccacgacc ggaaactggc cggggttccg
    35101 cggggcgggc cgcgacaatg tcgcgcgccg ggcgggaaag ttgctgaccg catggccgga
    35161 aaccggtccc gccgtactct ggcagattcc ggtgggggac ggacatgcca tgcctgcttt
    35221 ggccaacggg tgcctctatc tgctggacta tgacgaggcg cggggcggcg actgcctgcg
    35281 ttgtctgaac gcggacacgg ggcaggaacg ttgggagcac ctctatgcgg tcaaaaccaa
    35341 acgcaatcac ggcatctcgc gtaccgtggc cgcgacggac ggtcgctccg tcgtcaccat
    35401 cgggccgcag gggcatgtcg tgtgtctgga cgcggatgat ggcacatacc gctggggatt
    35461 cagtctgtct gaacggtacg gttcgcgcgt gccgctctgg tatgtggggc agtgccccct
    35521 gatcgatggg gatcgggtga ttctggcgcc tgccgcatcc ggggttctgc tgtgtgccct
    35581 cgcgctttcg gacgggaaaa ccgtttggga gacaccgaat ccgcacgggt acgcgatgtc
    35641 gcattcgtca gtgatgattc ttgaaacgga agggcgccgc cactatgtgt acgcggggat
    35701 cggcgggatc gcgggcgtgg gcgcggaggg cgcggaacgg ggaaccttgc tgtggcacac
    35761 ggaggccttt cgtcccagtg tggtcgtgcc gtcacccgtg ccgctgccgg acggccggtt
    35821 cttcatgaca gcggggtacg gctcgggtgg cgccctcttc cgggtgtcgc gcgtttcaga
    35881 cggttggcgg gcggaaacgc ttttcaaaac cgaccgcaag acgttcgcga gcgaacagca
    35941 gactccggtt ttccttaacg ggctactcta tacggttctg cccagcgatg ggggcggcga
    36001 gcggcagcaa ttggtttgca tgaccttgga gggtcagcgg atgtgggcga gcggacgcga
    36061 ccatacattc ggactcgggc cttacgtcgc tacgccggaa gggatgttgc tgctgttgaa
    36121 cgatgccggt acgctgacac tggcgcgggt ggggcgcgac gggtttgtcc ccttggcgcg
    36181 gcatgctctc atggagggca ggggacgcga tgcctggggt cccatggtgt tggcggacaa
    36241 ccggctctac ctgcgcgaca gcaagcggct cttctgtgtg caagtcggcg caccatgagg
    36301 cggccggtgt tcagacgaca cgctcgaccg tgatttcggg gtcgatctgc tcgcgcaaaa
    36361 cccgttcgat accctgtttc agcgtcatgg tcgcgtgcgg gcaactgccg catgcgccgc
    36421 gcagacgcag acgaacggtc ttgtccgtga ttgaaaccag ttccatatcc ccgccgtcag
    36481 cctgcagcat gccgcgaagt tcttccagtt ttgccttgat ttgctcgtcc atcattgttc
    36541 cttccgcttg aaatggtggc gtatcatgcc cgaaaacaca gggtgcgtca agttgcggtt
    36601 gacgggttcc ggtggccgcg ctaaggttgg gttcgtgaat cacaaagccg aaactaccgt
    36661 gttgctcgcg aacggcgcgt atccggtcca tccctttccg gtagacgcgc tgcgatcggc
    36721 ccgccgtatc gtttgttgcg acggcgcggc ggaccggctt tcgcaagccg ggctggagcc
    36781 tgcgtgggtc attggcgatc tggacagcct ctcgcctgcg gcgcgcgtcc ggtggcccgg
    36841 ccgcgtggtc gcggtttctg atccggagag caacgacttg accaaggctt tccgcttttg
    36901 cgtatcccgc ggctggcgcg atctggtgat cgtcggcgcg acaggccttc gcgaggatca
    36961 taccttgggt aatctctcgc tcctcccgga tttcgcgcaa gaggcggagg tgctcttgtt
    37021 gaccgatacg gggacgttcc ggccgatgtt gcggtcagcg cgcctgcgcg cccatcctgg
    37081 acaacaggtt tcgattttct cctttgatcc ccgtaccgtc atcacgtcgg aagggttgcg
    37141 ttatccgttg cggcggttac gactctcccg ctggtggcag gccacgctga atgaggcctg
    37201 cggcgacgag tttgatctga tattcgaggg aggccccctg ctggtgtttc agacgtatga
    37261 gatacggccg aaccgtgcgc cggggggagg cgatggggaa atggcggggc aacatgaatc
    37321 gaattctgtt tgagcccggg gaattgtgcg cggatgggac cgtgacgctt gcggacgcga
    37381 gggccgagca catactgaat gtgctgggac tcggcgtggg gggcgtggtg cggagcgggg
    37441 ttgtgaacgg gagtgccgga acgtcgcgga tcatcgggac ggagggaaag cgaatccggt
    37501 tgcagaccga tcatacgcag ccggcggagc cttctgggtt cgatttgttg ttggccgcgc
    37561 cgagacccaa agtgatgaaa cggctttggc cgcaattggc ggcactcggc gcaggccggg
    37621 tggtggtgct caacgctgcc aaagtcgaaa agtgctattt cagcagtcaa tggcttgatc
    37681 ctgcgcggat ccgtccgctt ctgattgaag ggttggccca ggccggcacg accatgctgc
    37741 ccgaagtgcg ggtgcgtcag cggttcaagc cgtttgttga ggatgagttg ggcacccttt
    37801 tccccacgcc gttgcgtctg ctcgcccatc ccggaccggt tgtcggtctt gccgcttccg
    37861 aatggccgtc cgggacgcgt ccgctgattg cggtcggccc cgaaggggga tggaccgatt
    37921 acgagcgcgc gatgctgacg gagcgcggtt tccgtccctt ttcattgggc ccccggactc
    37981 tgcggaccga tacggcgtgc gtcgcgctga tcgcggtgct ggcttggctt cgcgactcct
    38041 cccgctggag cggcggagcg gatgaatcgc gtgttttgtc cgggcggttg gggattggac
    38101 aggttgccgg tccttcatgt actataaacg ccgaacgcag aacggaaaac catgaaacga
    38161 gaacgctggg ttcgttatcc tcagaagcgc gatacgccga ctgagtgccg cgactggatt
    38221 tacggtcgtc ggccggtatt ggaggtgctg aaagccggta gacgccatct gtatgaggcg
    38281 gttttgcctc ccgccggacg cgacgcgccg gaaatcgccg aaatgcgtga cctgctgctg
    38341 gcccggggtg tgccgttccg cacgttggag cggagtgagc tggacgaatc ctgcgagggc
    38401 ggcaaccacc aaggtgtggc cgtgcgcacc ggagggtatc cgtacatctc gttcgatcag
    38461 gttctcgacg atgtgaaaac ggatacgaac gccgtggtgc tgattctcga ccacatcgag
    38521 gatccgcaga atgtcggttc gctgttgcgc acggcggatg cggcgggcgt cactgcggtg
    38581 ctgattccga aagaccgcgc ggccgacgtc acacccgctg ctgtgcgtgc ctctgccggg
    38641 gcctccgagc acctgcgtgt ggtcaaggtc gtgaatctgg tgcgtgccat gaaagaactg
    38701 caggcgtgtg aagtctggat cacgggattg gacttcggac cggacgccaa accatatacg
    38761 tccatcgatt tcaaaggtcg cgcggggttg gtggtgggca gcgaggggcg tggcctctcg
    38821 cggctggtgc gcgaaacctg cgacttcatc gccagcctgc cgatgaaagg ccgcgtcgaa
    38881 tcgctcaacg ccggcgtagc cggcgcgatc gcactctacg aggtggtgag gcagcggggg
    38941 caggcgagtc gatagaatcg acggaatcga tcaatcgaat ctctcgattc catcgaaaac
    39001 aaaggagcga tcatgagcat gttcggcggc acaggcggct atttccggct ggattcgtgg
    39061 attctggcca acatcgtgca actcgggacg cagcggttct gcaagcgttt cctgaaccgg
    39121 acgaacgatc cgtgcggacg gcagtacgac cagatggcgc aggcggcgcg atccggttgt
    39181 tccaacatcg ccgagggttc ggcgcgtcac gcgacttccc gcgagacgga aatgaaactg
    39241 accgatgtcg cgcgctccag cctcgccgaa ctggcgggcg actacctgaa ctggcttctg
    39301 cagcaggagc aggttccctg gggcaaggcc acgcccgagt cgcgggcggt ttattccgta
    39361 cggctggaca aggcggatta cggcgaggac gtgatccatg acgcctgcgc gcacattctc
    39421 gcgcagaagc ggaagttcgc gcagtggctg gagtcggaga gcgacgtgac gatggcgaac
    39481 gcgctgctca tcctgatcgc gcgggtgatc aacatgctca accaccagat ggagtcccag
    39541 ggagaggcgt tcaaggcgga aggcggcttc cgcgagaagc tgaccgggat tcgggtcgaa
    39601 gcccgggcgc ggcagcaggc cgcgccggtt tgccccgatt gcggcaagcc gatggcgcgt
    39661 cggaaggcga ggagcggcaa gaacgcgggc cgagagttct ggggttgcac ggggtatcca
    39721 gagtgcaggg gtgcgcggga aatggaagga ggagagaaat gaggagagtg ggtttttcga
    39781 gggaatcgaa tgaatcgagg aagtcgaggg aatcgattcg gtcgagggaa tcgattctgt
    39841 tgattcggtc gaaatcaaca ccatgcgaac tccattagag agtctggtgc ccaggccacg
    39901 ccggattgag tcggggccgg gagttttggc actgggcgag gcgggtgtca ttgtgggcgc
    39961 gggtgcgccg gacgatctgc ggcccgcgct gcgggtgctt gaggcggcct tggggtcagg
    40021 gtggccactg tcggaagcgc gggcatcctc gccggccggg ggcgtccgtc tcgtgttccg
    40081 gattgtgccg caggtggccg ggccacagtc ctattgtctg cgcgtcacgt ccgcaggtgt
    40141 cgaagccggg gcgcaggatc gggcgggatt gttttatgct gcccagacgt tcgctcaact
    40201 cgcgcgcttg tcgggtgggg tactgcccgt ctgcgagatc cgggatgcgc cggattttcc
    40261 ggtgcggggc gtgatgctgg acatcagccg tgacaaggtg ccgacgatgg acaccctttt
    40321 cgggctggtg gacatgctgg ccggactgaa ggtgaaccag ttccaactct acaccgaaca
    40381 cacgttcgcg taccgggctc atgaggaagt gtggcgcgag gcgagcccga tgacgccgga
    40441 tgagatccgc gcgctcgacg cctattgccg cggacgatgt gtggaactgg tgccgaacca
    40501 gaactcgttc ggccatctcg aacgctggct taccctgccg cgttacaacg ggttggcgga
    40561 gttgccgcag ggcggcgcgc cgttgccgtg gggcggcgtt cacgagaagc cggcctcgct
    40621 ctgcccgacc gatccgcgca gcgtcgattt tctggccggg ctttacgacg atctgttgcc
    40681 gaattttacc tcgcgccttt tcaatgtcgg atgcgatgag acgtttgacc tgcggggcaa
    40741 cgggcgctcg caggcgcttg tgaggcaggt gggcgagggg cgcgtgtacc tcgattttct
    40801 caaaacgatc catgggctcg tgaccgcgcg cggcaagcgg atggcgtttt ggggcgacat
    40861 cgtgatccgc tatccggagc tgattgcgga gctgccccgg gacgtgctgg cattggagtg
    40921 ggggtatgag gcggaccatc cctttgacgc gcactgcgag cggttcgcgg cagcgggcgt
    40981 gccgttctat gtgtgccccg gaacctcgtc gtggaacagt ctggcaggcc ggacggagaa
    41041 catgctcggc aacctgcgtt cagcggccga gaacgggctc aagcatggcg cgtgcggatt
    41101 cctgatcgcc gactggggtg acggcgggca ctggcagccg ttgtgcgtca gctttgcggg
    41161 ttttttatat ggggcggcgc tatcgtggca tgtggagggt aaccgggaac tgccgctggc
    41221 cgcagcgctg gacacgcgct tgacggaggc gggacggggc cagctgctgc tcggattggg
    41281 tgacgtttac cggttgtgtg gcgcgttacg cgggaacggt acagaactgt ttcagatcct
    41341 ttcgaaaccg cagtcacgcc cggtggcgca gggggtgacc ggtaagacgc tttgcgccgt
    41401 gctgagccga gtggaggagt tggaacgctc aatgctcact gctcaacgtt cggtggttga
    41461 gcaggagacg gagcaggtga tccgtctgtt gcgtgcggcc tgccaccgtg gcttggcctt
    41521 gcttgacgga agcgtgcgcc gcgctgccac ccggcgcgcg ttgcggacag agacggatga
    41581 tttggcgtgc gcccatgccc gtgtctggcg gttgcgaaac cgtgaggggg ggctggccga
    41641 cagcttggcg aagctcacac gccaccgtca ggaatacgcg gagtgacttc gtcgttcaag
    41701 cgtttgaaca gattcatgat ttcaacactg gaacacaaaa ccttgcctat aa
//
LOCUS       DAOSLE010000008        41742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1093_length_41742_cov_7.596709, whole genome shotgun sequence.
ACCESSION   DAOSLE010000008 DAOSLE010000000
VERSION     DAOSLE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 41742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41742
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_41742_cov_7.596709"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PK576_01240"
     CDS             <1..530
                     /locus_tag="PK576_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOE60245.1"
                     /translation="PDRMGRASPLELDFGAIGKGYALDVACRMLTEDWDFADFLMHSG
                     TSTVIARGTMGDGQPGWPVSVGGDWRERAGLHHVRLSEGAISGSGFDVKGAHVVDVRR
                     GVAASRHPAAWSYAPAAAVSDALSTACLGLGWREICAACAALEGCGALVARAQPVWMD
                     RLRSPVRQYGAFPAV"
     gene            622..1290
                     /locus_tag="PK576_01245"
     CDS             622..1290
                     /locus_tag="PK576_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOE60246.1"
                     /translation="MKHTGMMLAAVVAGLAVVAGAQDGAKVQLKIELPKPQFTGTPKD
                     IRSPNLEPARGGKPRPPIDVPAGADKVLSRDCKVTSSDPEPIIGELSFITDGDKEHDA
                     ATYVELGPQTQWVQVDLGKEKEIYAACVWHYHGEARVYRDVICQISNDPDFIDGVVTV
                     FNNDHDNSSKLGEGKEKEYIETNEGRPFAISGVKGRYVRFYSKGNTSNEMNHYTEIEV
                     YGKP"
     gene            1542..1973
                     /gene="trxA"
                     /locus_tag="PK576_01250"
     CDS             1542..1973
                     /gene="trxA"
                     /locus_tag="PK576_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE60247.1"
                     /translation="MRMRIFATVSVIGCLWLTGCKPAGAPTTDSVPDAGSGTAAQVLE
                     LNDMTFTAQTAQGVVLVDFWAPWCGPCRTQGPIIEQAAKLLGDSAKVAKVNVDDAQQT
                     AKRFGVQGIPTLIVFKDGKPGKRFVGVTQAAVLVEAVKEAL"
     gene            2102..4843
                     /gene="polA"
                     /locus_tag="PK576_01255"
     CDS             2102..4843
                     /gene="polA"
                     /locus_tag="PK576_01255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOE60248.1"
                     /translation="MSETAKLFLIDVMPLLYRGHFVFLKSPRVTSGGVNTSALTGFAN
                     CLLQILNEHTPTHVALVLDSSTPTFRHEAYPLYKAQRQKMPEDLAAAIPMSIELAEAL
                     RIPILRVDGFEADDVMGTLAAHAATKPGWQTYLATPDKDVAQLVGPATFLFRPGKGGA
                     PAEIYDEAAVCRHWGVSAPSQMIDLLGLAGDASDNIPGIPGVGEKTATALLAQFGDLE
                     NVLAHAAELKGKLAEKVAAGAASARQSRELAAIRTDVPLTIRLDDLACRDPDEARLRA
                     VLKKYELAALGKRLLGEGFTDGTGGVAASGAYQTLADVPHTYVCVTDEERMRELLRAL
                     EEAPEWAFDTETTGLNPRHDRLVGLSFATAPGRAWYVPVPETHEACRAALHRFAPYFD
                     DPATIKIGHNAKFDLTVLRQYGITLRGGLHDTMLAHYVIDASDRHGMDHLARQYLHYN
                     PIPITALIGEKKRGTVQGNMGDRAPEEICDYAAEDADITLRLDRLLRQEAQETGCLRA
                     LTECEEPLVGVLLDMESEGIRINEQALREYGRELDREILDLEIRIREFGGGPFNPASP
                     KQLGEVLFDHLKLDPDAARTASGQYATSEEVLLKLRDRHPIVPLILEYRMCGKLKSTY
                     VDKLPDCIDPETGRIHTSFSQALTETGRLSSSDPNLQNIPIRTERGQRIRSAFVARDA
                     DHVLLSADYSQIELRVMAALSKDPGMIEAFRRGADIHRETASRVYGVPPDEVTPEMRS
                     VCKMVNFGIIYGISAFGLGQRLDVPRKQAAELIETYFTTYPGVKTYMDQAIAEARAKG
                     YAQTLLGRRRFLRDINSRNATSRQSAERNAINTPVQGTAADLIKLAMVRVHRELAERG
                     LKTRMVLQIHDELLFDAPRGEVETVRELVTQAMTTVWEIGVPLEVSVGTGETWLEAH"
     gene            4898..5491
                     /locus_tag="PK576_01260"
     CDS             4898..5491
                     /locus_tag="PK576_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE60249.1"
                     /translation="MNEKEGQEVTRRNALAWLLRVGAGAAITAAAVQATRRASGKRTV
                     WQIDPRKCIQCGRCETECVLHPSAVKCVHGYAMCGYCKLCFGYFQPDAARLDEAAENQ
                     ICPTGAIRRQFIESPYFEYHIDEARCTGCALCVKGCTHFGNGSLYLQIRHDRCVGCNA
                     CRIAEACPAQAIMRVPADTPYLLKEKLGPSGPKESDG"
     gene            5488..6798
                     /locus_tag="PK576_01265"
     CDS             5488..6798
                     /locus_tag="PK576_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE60250.1"
                     /translation="MKRPGCIRKRAGLAGVLLPLVTWAADPRFPQPQFESGHTLPQTP
                     HPAFVEQVPAWMDVLLLALALGIAVWLVRRLRSRRGVLLFSLLCLGWFGFVRRGCVCP
                     VGAVQNVAEAMWNGGGLPWFVAAIFVLPLFATLLYGRVFCGAVCPLGALQDLFIIRPQ
                     RVPRAMDAVLRLVPLGVLALGVVAAVNGAGYLICQWDPFVGLFRRSAPMTVMLTGMAV
                     LFVGMVVARPYCRYFCPYGVLLEFCAGLAWRPVTIPSRTCVNCRLCVGTCPVGAVTAP
                     REVTSGAERKRLFRRFVTLAVLMPLGVLLCASTGWLTGAWVARAHPAVRMAEVLATVP
                     QEAADQHPELEAFRRGGASRDEAEAYAAEAVRRFRAGGAGAGVFVGVVMGLRLLGMTR
                     LRRRPLHEADRMRCVACGRCWDACPLNRSVAGTSGSTSDGPSVG"
     gene            complement(6787..7638)
                     /locus_tag="PK576_01270"
     CDS             complement(6787..7638)
                     /locus_tag="PK576_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE60251.1"
                     /translation="MANSDRDMPLLRIRGLKTWYPVKKGVFARTVGHIRAVDGVDLDL
                     MRGETLGIVGESGCGKSTLARTILRLEKPRAGTLLLNGRDIRTLRGADLRAYRRTVQV
                     VFQDPYASLNPRHTVLDLLTEAMLAHRLVTPETRRDAAVRLLADVGLPEEILDRYPHE
                     FSGGQRQRLCIARALALGPQLLICDEAVSALDLSVRAQVLNLLEDLKAKHGLSYLFIT
                     HDISVVQHIADRIAVMNRGRIVETGPAEQVLNTPEHPYTRLLLSAVPRIGVPLPDFPE
                     PALTSTH"
     gene            7821..9065
                     /locus_tag="PK576_01275"
     CDS             7821..9065
                     /locus_tag="PK576_01275"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE60252.1"
                     /translation="MNKKTLRDVDLKGKRVVMRVDFNVPIKEGVIKDDTRIRGALASI
                     RYVLEQGGSLVLMSHLGRPKGKGYEEEFSLKPVAEHLAGLLGKPVTFAPDCANADAEV
                     SALQPGGVVMLENTRFYKAEDGKVKKTDEMSDEEVARKKAELKAKREELAKKLASYGD
                     LFCNDAFGTAHRAHASTAVICKFIPESVAGFLMEKEIEYLGNAVEKPTRPFVAILGGA
                     KVSDKLAVVKNLLDKVDTLIIGGGMAYTFLKAQGHDVGGSLCELDQLAYANQMIEKAK
                     ANGKRLLLPVDNVVADKFDAEANTQVVGNDIPSGWMALDIGPQTTELYATAIKGAKTV
                     VWNGPMGCFEMAKFAGGTTGVCKAVAESGAVSIIGGGDSVSAVNKSGLADKMTHISTG
                     GGASLEFLEGKELPGVAALSDK"
     gene            complement(9215..11632)
                     /locus_tag="PK576_01280"
     CDS             complement(9215..11632)
                     /locus_tag="PK576_01280"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOE60253.1"
                     /translation="MTTSRPTLLRCLLLVAAGLVSATDLTLQNRAVLLQYREASGEVT
                     LWVPGESKPFARFDAATQKPSAVRTVRLNEPRLGAGEALEFDFPHGSRNRVMLFADLP
                     FAVLRTSWRGTNTTNPVNRIAYPAVRLALDRPAEELTSLGTGGLLQPKRNPGSYMWTA
                     VADPATRRGVVAGWLTTDRGSGIVRMAVAEECVTLLPHLDYGRLAPNADREEDLELFA
                     LGLFDDARFGLEAYADAIARIYAIRLPPMPTVHCTWYVDRASNERVMTDRTAYAAQTL
                     KPFGLRVMQIDDGWQSGQSTNGPKKVFIRHRPDGPYPAGMKAMSDTIRAAGMTAGIWL
                     IPFAGSREDPWFADKTAWFAKRSDGSPFDTRWGGTCFDLTRRDTQAYLRDMIRTLTAE
                     WGYRYLKMDALYAGAAINLNYVCNTFREDEIGQAILTDPSVTPIQMMRNSLKIVRDTA
                     GRDVFLLGCCTPQNMRSAGAAFGLVDAMRIGPDNGANWSAMMRGPEYGAWNYFLHGRV
                     WYNDPDPLYVRPSLPIHQAQALAAWVTLSGQMNSSSEHYAGLPPDRLDLLRRTMPSHQ
                     AVARPADLFENRIPGIWLVTDGATAERPQRQVAGLFNWDGTNKTFNVPLASLGLETRS
                     PWIGFEYWGNTLTPPFRHSLPLTLPPQSSAVLALCPIRDRPQLISTSRHITQGITDVR
                     AVTWDPVSRTLSGVSDTVADDRYELRILTHADASASFGTVLSAAITGPNGYELTIAPG
                     EGQVTRPDDAYAHANTPDAPRTTLFEPKSEEGLVRLAFISGSGGQVSWRVIFSERTIS
                     ALTPPAP"
     gene            11926..13272
                     /locus_tag="PK576_01285"
     CDS             11926..13272
                     /locus_tag="PK576_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE60254.1"
                     /translation="MRLTGRGLGTMWLAAVCAAAGIVSAAPEYRQVAAEEVRPRGGIG
                     NVMRKIRAGGEVTVAYLGGSITSMQGWRNKTTDWLKATYPQATFREVHAAIGGTGSDL
                     GVFRLGRDVLAQKPDLLFVEFATNDGGQAPEQIWRSMEGIVRQTWKHDPLTDIVFTYT
                     ITTAMTEDYLKGMCNRAASAMELLADHYNIPSINFGVRVAQLLKENKLIMSAREIETA
                     VPKESPDRDRLIRERIAADPRMLFANDGVHPRDEGHELYKAAIIAGLTQMRDLPAVDH
                     KASLANVFVQDHWEAAKMVPIRASMLNGNWKALPEDDPKQKSFGKRMGQIWMADQPGS
                     TLRFKFKGTVAKVYDLLGPDGGQVTVSVDGQTGAKPVPRFDSYCTYHRIATLGVFTGS
                     GAEVTREAVITLHPEQPDRSSISFRLKDPAKELAAPKFQGTRIWMSQIMLIGDLVE"
     gene            complement(13302..15719)
                     /locus_tag="PK576_01290"
     CDS             complement(13302..15719)
                     /locus_tag="PK576_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOE60255.1"
                     /translation="MKTQTFVRYLLCLALTLPVLTGCDRVPKGLEPEKLVLFSSETLL
                     GMPGTNMVNAIHVELLSKPVASLLGGAGTQHPVAGKRINAIPVDPAGGVRVSPPSQLT
                     DAGGSCKFDVTFGAVFGDHYLDLVCEEAPEIRKRLRFVCGVAVENVRQEVSAGDPLPK
                     PLRVRLVAPDGKPLADAGVHFTLTQQPGKHGRIGTSFARTDPDGIAEVSLKTDPHATG
                     TYEVRAEVADPKRGLFTRPVFLQAMSICLFRTLIGVLGGLALFIFGMTLMSDGLQQIA
                     GHRMKAVLAYVTRNRVAAIFAGTFITALIQSSSATTVMTVGFVNAGLLSLKQAIGVVF
                     GANIGTTVTGQMVSFNLDNLAFPAIILGVTGLLLCRKATLQGTARTILGFGLLFYGMT
                     LMSDELKSVSTFPSFVRFFHIFDCAPQSGQTMPIGAVLGAIGIGTLMTMLVQSSSATI
                     GLAIALANSGLLNIWTAVPIVLGDNIGTTITAVLASINANRTARQTAVAHSLFNVLGT
                     CVMVGLFYVPFHNVPCFLYLVNSVTAGNVFLGENIGRHVAMAHTLFNVTNVILMTPFI
                     GALAWLCEKIMPGKKVTSRVVHLEKNLLSSPPLALFCAMRALGDMTETAWNAAARSLR
                     GYKDGKPVDVNSIQEIEDEVDACQTGIMDYLVQLTRKDLSEKQAAAIPALMHCVNDAE
                     RISDLALLIAKRSASLTSENTKFSDTALQELEALFDKASVIANLTRESLQDGRLLAKA
                     VEVVVNDLKAMARQSIQGHVERLQKGLCTPERGLVYVEVVGALENIVRHLENIAQRSD
                     ELTAAAT"
     gene            complement(15815..17779)
                     /locus_tag="PK576_01295"
     CDS             complement(15815..17779)
                     /locus_tag="PK576_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOE60256.1"
                     /translation="MLKISTLDAVIIFGFLFSVIAIGSAAARRSSRSAADFFLSGRSM
                     PWWLLGVSMVACTFSCDTPNLVTDIVRTQGVAGNWAWWAFLLTGMLTVFVYAKLWRRS
                     ALDTDLGFYEIRYSGRPAALLRGFRALYLGVFFNIMIMATVSLAAIKIGQVMFGLSPV
                     ATLLYASAGVGIYATLGGLTGSIWADFYQYSIAMVGAIFAAVYAVKSADVGGITSLKQ
                     LFSQPEVQTHMSMFPSGEGGLSALMTVLILPIAVQWWNVWYPGAEPGGGGYIAQRMLS
                     AKDEKNAVGATLLFNFLHYAVRPWPWIIVALVSLVHFPLTPPAEQAAATRWLADHPAQ
                     VGQYDTAKMQMPEPEREQVRQAKADAAGVGSLARAFPKVDDQFLRHDIAYPGMISTMP
                     KGWLGLIVASLIAAYMSTIATHLNWGSSYVVQDFYSRFINPNSTPRQAVAMGRVTMLA
                     LLVLSGLVALWMRNAKDSFDILLQIGAGTGLLYILRWFWWRINAWSEISAMVISFAVA
                     CFFQWGAAPLGLDAAMERAGLFAVMDYSAWKLVIGILITTIGWLTVTYLTPPENQDLL
                     ARFCTRIRAGGPGWRHIEEASSLPKSAEGWDVPTGLLCMMIGCLAVWSALFGVGNLLY
                     GRMFAGSFLLVVTVVATALLMRLVGKIRLS"
     gene            complement(17843..18706)
                     /locus_tag="PK576_01300"
     CDS             complement(17843..18706)
                     /locus_tag="PK576_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60257.1"
                     /translation="MRLLPNNVWFCLCLIGITLSGTAEVKRSARNPQRLTGQPAQRRS
                     NRSGQTASADKTLTQTAAPQNRVTRVVFMGPKSGWGFTKREVSYYSPEGKRLGTMPGG
                     TLFRYSDVKASSKNPMLLSRVRRNNAWDEPCLLDCTETATYEGDPETVDPEIVGNLHS
                     FFLLTAKIAAYKATLAEKAHAANPHFLAARDAQRAYQQSLAQADRLEAEAQTCTGARK
                     VKVLDALRALKYEQVRLQSEVTRTSAAFTKWNQTHPAVVPADDPTLRTLEREREAVRV
                     KVADLIPPDAS"
     gene            18836..19009
                     /locus_tag="PK576_01305"
     CDS             18836..19009
                     /locus_tag="PK576_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60258.1"
                     /translation="MLRQNKRPCPETRFARGVRALRKLTPGLTDRERAALWLVLALFV
                     LGALVRWLRMQDG"
     gene            complement(19075..24648)
                     /locus_tag="PK576_01310"
     CDS             complement(19075..24648)
                     /locus_tag="PK576_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60259.1"
                     /translation="MKRVSNDLCAALAVFLTCTGVASGVTNLLPVARILLFDDYADTS
                     LWTHEGPVITGLATRRALRSGTNHFHSAGWNIFNTRTGSARGQGSGLIVPPTLPSFNI
                     NTATNFAANLQNTLDAAIISPVFQEGIGTVYFEAVNNQAGRPTRITIWLTTNMTDQTS
                     GWPTNILLDAETQDLTYNWVQFGDDVVLDYSNSGEFYRYVRSLNYRGPAKLRWQRSGG
                     VSGGSALPPPEAENYGGVYTDAPGDGLDSFFTVIDNIRVSQPPSDVVVRKTACPFQPG
                     YPSVGTNMTVRCFTDNVDMNTPTDWTSRTNKVYYRWRYLDQASNDWTSVTLTNFFQGI
                     QGNNEGYEGVIPPHPRTGDLEYYFECSYAGYSYQSPDYTGEGHVYPSEAAAAGFSPRL
                     LGSRSQPFSIRIRPFVSPYGSISVVADEYPEPVTMELVADNTWQGMLPLSDAGTTNVS
                     WRLKGERRYLPVEVAYSTSVVHWAETLPGTINRLPYGGVCEETETPLTKTVTADRGAY
                     VMLTFDTETLKYMLVRAEYQNFNNWPALSDKFSESNGQTPKASFLNTFEAWPVSVEDT
                     YSQYFHLPVPTTNVFVREPFGTPQANWMAGHAAFSVERTLADENNKPAGYVNFRNQAL
                     RLQGGGCTLGLGYVHNTVQTRTDGIRDFAFKYRLAQTADKIDVAYDRTAFISSNYSAK
                     VTARSYTGHSPDAPSLSVIAYYQDADHFYEYRITQVPDTDNLSRDKRVTHQLYRWING
                     TPTLLATSGTYDTGNAGDIAGFEHAMEIRIFNQPNSTLIRCYYYPSSSAVLRIDHTDN
                     SDSRLTYGTYGVLSEDCGAGFSNFIIQPTTTGATLTGSATTVLSDTNAFNTDKNNWSA
                     PWGRFVLDGDRSPKGIHAVIPTQKLGVYLQSSAQDSTAEPAAPGTTAWKLFQEITVSG
                     YGYKTNAVTVKDWKTQFLLLQVMGVAGSKADVVVDELKITSWRGEQFGSGNDDAWLAT
                     EAWAVSNNAANGHVIQLKHTRADPNADQAVRSILLENGMGMLEFDYRVLRAPAQLTVQ
                     YATERENDIWIPVHTYAISNVMSEWDHISGYVGITNPGFLRVINDRSGGCSNAWVEID
                     NVRAWEEPVVKDTSWRAYNVKVTQTDKQRIMLDGEEACLLNNSTTQDAEPIQDQNVPF
                     VQSPVLPYGLGELSFKTRAYTNTFPSSLYIYATTNGWNAPTNLWFEIAAFTQITNRFY
                     ETVTVKPVDGRDYDAIKFISPLGGQRACIEDIAVTEPVFPGFDIENVQVLCRNGDSYD
                     DDPPRHQPINSDHVGIQAELVNIRLSPSNIQMYVDYYIGTNVWGVENWPAAQVVTLPM
                     EQMGNSTTYQTLPSQDLPMLDSDTIVQYRVRATYLGGIPLTAEQKTFENPPWYFPVDF
                     NAVYASQGWSPYYIVYGVPLKTVWINEVNAAEHVEENGVPQYGFYDNPYIEIAVPVGV
                     DLAGWSIDLVMNDRDMHTITLPEGLPEQTAVTNGYAFFVIGEKFPDPNRTPVPLPKLD
                     YGEPGFYWYIPCIYAGGLRLKRPMGMYEHVIAYDDPSQGSGPEFDGELWAAEDPERQF
                     VYVGPETHGGSLNVTNGTGVTRADWTFPLYWTPGGLNPGQSVAFPELGVSNHWIVSTL
                     NALNGTQNGLRTQCHTFRTRNGANTNILYQADNWYRLYSVKVNQVEQLPPGEGLQNYL
                     LPLTNVSANLNVEAHFALRQDIADLEISDDMLNWLLGFGDNNTPLASSYFFGNGRLMS
                     LTELYWLNADPTVTNRVDGSIVRFTRDAETNYYVTASLAVNGQNCTCLLSQAVMKLEM
                     TPSLTIPDWRIAGQYRLMPDSFDTNHQCRILLPNPYIYSFPYWDPAQAFFRWVIEEQH
                     PNIGVLPLVNTPPEP"
     gene            complement(24716..25591)
                     /locus_tag="PK576_01315"
     CDS             complement(24716..25591)
                     /locus_tag="PK576_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE60260.1"
                     /translation="MLFVRKTGFTLIELLVVISILGILAAALTTQVGRARSMGQAIRC
                     KTNLRNLAQASLSYAVETKHMPWAGSFQVRYPSRDGNRYVPKYHEHRGWVGWVGSGSW
                     PNKDIQNMEISCFHGNNAYYSLTNGSLWTFVGKDISTFLCDTHKKAAARTGLEKIHRS
                     YVMNAYFGYEHKAPPKTADVRRWLESLSSSGNAASRLLFAELPARSIVSSGEGADSVL
                     QAEIKNYANPGSPEMIGFNHKVGKRYVAHVAYADGHVDALIEPEGGNKDDCIELTRQL
                     CNGEDITEELRDRMR"
     gene            25754..27511
                     /locus_tag="PK576_01320"
     CDS             25754..27511
                     /locus_tag="PK576_01320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE60261.1"
                     /translation="MRWSRALLNTLRDDPQEAEINSHKLMVRAGLIRKVAGGLYTFLP
                     LGLRALHKVTRIIREEMDRAGAQEILMPALQPAELWERSGRLAAMGPGMFRLKDRAER
                     LMTLGPTHEEVVTSLLAGEVSSYRQLPCTVYQVQTKFRDEIRPRFGLMRAKEFIMKDA
                     YSFDTSMEAADRSYQAMYDAYTRIFQRCGLRVKPVEADTGDIGGSWSHEFMVLADSGE
                     DGIVDCAACGYAANQERAERMGKPAAEAACPPLSEVPTPGVRTIEDLTAFFDCAPDRF
                     IKTLIYLADGKPVAALVPGDRDVNEHKLKRLLNASKIELADEETIFRVTGAPVGFAGP
                     AGLSIPVYADQSLEGAEDRVTGANRADTHYRQVSLARDARVTAYGDLTVVGSGDLCPR
                     CGKPLQLRRGIEVGHVFKLGTKYSESFDAVYLNEKGQSEQMVMGCYGIGVTRTLQAVI
                     EQSHDADGIIWPVSVAPFAVSLLLLDPRDERVYDVVAQLEAELEACGIDVFVDDREER
                     PGVKFKDADLIGCPVRVVAGAKGLAKGGVEIKLRTGKESEIVPVEQAAAAIADRVKAL
                     TSALSAKADEPAACEAGNG"
     gene            27557..27883
                     /locus_tag="PK576_01325"
     CDS             27557..27883
                     /locus_tag="PK576_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE60262.1"
                     /translation="MKPSKHDKAPERVALKKRDLVNRIAKDTGLTQLQVLDIVQRTLD
                     GIVDALEAGQHVEFREFGVFSVVKRKARIGRNPNQPADVVTIPVRYAVKFKPGKRMRD
                     MLADKV"
     gene            27915..29192
                     /gene="hisS"
                     /locus_tag="PK576_01330"
     CDS             27915..29192
                     /gene="hisS"
                     /locus_tag="PK576_01330"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOE60263.1"
                     /translation="MNVPASFSFDPPRGMRDFYPEDMFWRNRIFDAWRQAALAFGFQP
                     YDACVVESLDLLQRKSGEEVSEQIYHFEDKSGRRLALRPEMTPTLARLIAARQGQLVF
                     PVKWFTVAQCFRYERMTRGRKREHYQWNLDVVGEGQVSAEVEVLAAAAEGLRRMGLPE
                     QAYRIHVSNRALLAELLAVSGIAPEHHAAVFLALDKRGKISDDEIAGLLRAEGLKDPA
                     VEQTFALLGIQTLDQAAERVGPDSAAVAALRELFALAGVYGIADRLMFDIAVIRGLAY
                     YTGIVFEAFDSARKFRAIFGGGRYDNLLTTIGGAPTPAVGLGFGDVVIAEVLKDLLSE
                     TAATEKNGVIIGYMFPEQRESAMRLARSLREAGERVDLHLARQKPKTFFAKAGAGSAA
                     DAIFIGPDDVARGTVRRKHLATREETVIPLPEA"
     gene            complement(29281..30120)
                     /locus_tag="PK576_01335"
     CDS             complement(29281..30120)
                     /locus_tag="PK576_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE60264.1"
                     /translation="MKFTRRSFLRHSAATLSLTAIPSPGAHADKPKIRVGSCTLRLDQ
                     ARQAGLDGVQLWAGGAADELDIYKADTRARLKTQIRETGLPICSLMLGLLNSYPLATD
                     PRAPAWLRQSIEAAQDLGVPNILVAFFGKGDLQADRKIKEPEFASACRRIRDAAPRAH
                     DAGVTLSIENLLSAEQNLRMLDTIGHEAVSLYYDVYNTGKSMKYDSPAEIRLLKGRIT
                     QIHYKNGPRYLDEDRPYFEAVTAAIREIRYSGWIVLETSSPSKDGVADGKRNGDFVRS
                     LFA"
     gene            complement(30147..31142)
                     /locus_tag="PK576_01340"
     CDS             complement(30147..31142)
                     /locus_tag="PK576_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOE60265.1"
                     /translation="MHVKLDIPGFVDLQVNGFVGVDFSGPDLTEESFKLACHTLLERG
                     TAAFLPTVITGTPEQTRRNLERMASVLKKDAVLARHIPGFHLEGPFISSEPGAVGAHD
                     PAAVRPPDCAYFDRLCQWAGDRIRLLTIAAEIPGAEVLTRHAVARGILVSCGHQLADA
                     AALARLVDAGASALTHLGNGCPNQAHRHDNPLLTGLAEERLQILFIPDGHHLPAHVLK
                     VFARAAGARRLIATTDASPAAGLPPGTYNVLGNQAVLEPDGRLHNPEKQCLVGSSATL
                     IACMNVLHRLNLFTLEELLQIGFRNPLALIRVESREVPTCRYRITFSPKTGFVLR"
     gene            31290..31730
                     /locus_tag="PK576_01345"
     CDS             31290..31730
                     /locus_tag="PK576_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60266.1"
                     /translation="MDWRAELSGILGGRARTTRAEEENAVFEAFLDTVAIPALSEIAE
                     ELVQKHERDAQVRRAPASATLSVRIGEMEEITFRVMKQFVQNGILPRAEVRLNRGQRL
                     VKYESMFRADPQTYPITEITQDEVIACFIKYYRMVMDRNPTAAE"
     gene            31800..33230
                     /locus_tag="PK576_01350"
     CDS             31800..33230
                     /locus_tag="PK576_01350"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE60267.1"
                     /translation="MMKIPLPKIIQGGMGVGVSHWRLAQAVSKLGQLGVVSGTGLDQV
                     LARRLQDGDLGGDVRRALSHFPFPQMAQRVLDALFVPGGRPADQPYQAATKHTIKSNR
                     WVDELCVVSNFVEVFLAREGHSNPVGINYLEKIQFPHLPSVYGAMLAGAAVMIVGAGI
                     PVEFPGVLDALSRHEAATYTVRVHGATPETDCQRVFDPALFFEDGCTPPRLLRPDFLP
                     IISSDSLATMLLRRASGSVEGFVVEGPTAGGHNAPPRGPLQLTPDGQPIYGPRDVVKL
                     ESMRKIGMPFWLGGGYGSPEALRTALAEGAVGVQVGTPFALCEESGLEPKMRHALIHL
                     ALAGDGKVFTDPLASPTGFPFKVARLEGTLSEPEVYTARRRVCDLGYLREAYVQADGV
                     IGYRCPAEPVEAYTAKGGSAEDTVGRKCICNGLFGNIELGQVRADGTREPGIVTLGDD
                     YLRIGRFCTLEQPDYSASHVVRVLLG"
     gene            complement(33281..34387)
                     /locus_tag="PK576_01355"
     CDS             complement(33281..34387)
                     /locus_tag="PK576_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOE60268.1"
                     /translation="MTPTLITTAADLADLNTRLLRAEAVALDTEFFWERTFYPVLGLV
                     QLATPDGCWLVDAVCLKDLSALAPLIASPSVTKVLHDAPLDLTILARATGAVPRSVFD
                     TRLAAGFAGLSATLSLQALLQEVLGVTIAKGETRSNWLRRPLSENQIRYAAEDVVHLL
                     PLREALLARCAGKRSRNWLDQELARLDDPAHYEESPPEERFRRVKGAARLNRRHLALL
                     REVAGWREREARRRDWPRNHVLPDPVMVTIALRGPADVRALMAVPGFPSRMPAKLVGQ
                     ILAAVERGKALPESACPDPSDRNPATARALKEPTDRLLAHLRIACEKEGIDPAWIASR
                     NEAESLVQSLARGDASAHPLMRDWRQTLLAGFTL"
     gene            complement(34398..35783)
                     /gene="lpdA"
                     /locus_tag="PK576_01360"
     CDS             complement(34398..35783)
                     /gene="lpdA"
                     /locus_tag="PK576_01360"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE60269.1"
                     /translation="MQDFDIIVIGAGPGGYPAAIRAAQLGKKTAIIEKEALGGTCLNW
                     GCIPTKTLIASAERYHHATQSATFGVTAKGVTFDYAAMTARKAEIVDKLSGGVRMLLQ
                     SNGVELINGTARFEGPNRLSVLQPDGTTRWLSAVSIIIAAGSDSAMPAFLPKDKRVMD
                     SRAFLDLTELPKKLLILGGGVIGCEFACMAAALGADVTVVEMLEDIVTVLDRDVRRVL
                     RKRMETLGITLLTGTPLTDIAVKKNKVTGTFKDQRVEGDVLLVAVGRKPNTALLDLAR
                     AGIKTDERGTIPVNAQGRTNVASVFAVGDLVTGSIQLAHAATQQGVTAAEAAAGLKSK
                     VERICPACIFTSPEIGLAGLTENMARQRGIAVKTAVFPFSALGKAMTAGETDGFVKWV
                     CDPVTGQVLGAQAVGCHATELIAEAALAIRNELTVHEIGNTIHCHPTLSEAWMEAAHA
                     FHDACIHLPKR"
     gene            complement(35841..37226)
                     /locus_tag="PK576_01365"
     CDS             complement(35841..37226)
                     /locus_tag="PK576_01365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOE60270.1"
                     /translation="MAQAVIFPKLGQTMEEGAIVQWLKQEGDPVVKGDILFEIETDKA
                     NLEVESFFEGVLLKIYVREGVTVPVNTVVGYIGAPGEKVPDQAPKPEAPAPAPKPVAS
                     PSPTPGAARVPVAEPAAAPAPVPARAPVPAPPSHPAPQPAKVPVPAARLVISPRAKTL
                     AQSACINPARITGTGPNGRIVEKDVRAYLAASGYDALRITPAAKRLAAEKSIDILTVR
                     GSGDSGRIMVEDIERAIRRKPVALSRMRQVIARRLVESKQTIPHFYVTVKADITDLMV
                     FRSDLKARGLNYSVNDFVLEAVVLSLEEIPIVNSVTDGQTVSWRGDVDIGMAVSVDNG
                     LVVPVIRAAQTLTFSELSAQAKALAEKARAGRLTPDEMTGSSFSVSNMGMLNVDQFTA
                     IINPGEAGILAVSSGRQEPAVVNGEVRIRSRMAMTLSVDHRIVDGAVGAQFANAVRSK
                     LENIELWKSLV"
     gene            37441..38562
                     /locus_tag="PK576_01370"
     CDS             37441..38562
                     /locus_tag="PK576_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE60271.1"
                     /translation="MITVLHVLEATEGGTRRHLRDLVGALEPLGFRCALAVSCRRDPA
                     FRRDVAFYLSRGLTVAELPMRRGLAPITDVAALLRLIGCVRRVRPSVIHAHSAKGGLL
                     ARAAGAVCRVPVVYTPHVFPFLMACDPRMRRVYRLVERGLRCMTAAVIAVSEEERREA
                     AALGYAASRIYLIPNGVDGKVPGEVTVRKDGELRVGFFGRLTAQKGPSVLLEAVADVV
                     AHLPQVRFVFYGDGTEADTLRARAEVLKLNDAVRFLGSVAQGEVVAHMRQTDVIGIPS
                     RWEGCPYVVLEAFQAGVPVVAAAVGGVPDLIRDGVTGVLVEPDNPEALCDALLSLLRD
                     PQKRRRIAEQARNELARYRLDAMAAAVADVYRRVTQDLP"
     gene            complement(38570..38788)
                     /locus_tag="PK576_01375"
     CDS             complement(38570..38788)
                     /locus_tag="PK576_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60272.1"
                     /translation="MGTGRTLNKAPRTRPIKSQAEKRRRLKAQRARLVKIGVDDSVVA
                     KMQPEEVRKLVQHPAKVKKSLATPNRSA"
     gene            38996..40330
                     /gene="rimO"
                     /locus_tag="PK576_01380"
     CDS             38996..40330
                     /gene="rimO"
                     /locus_tag="PK576_01380"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOE60273.1"
                     /translation="MSVSVGFISLGCSKNLVDSQVMAGYLKAGAIALAPSPEEADVVL
                     INTCAFIESAREEAAGAILRACAHKERGGCRAVIVAGCMVQRYRSRLKAAFPGVDAFL
                     GIDELERIGEVVGNVAKGKRCGVIAARGMPVKCYKPPYPTLLFTGGPFAYLKIAEGCD
                     HRCAYCAIPNIRGAYRSRSEDELVHEAQAMVRAGVREINLISQDTLRYGTDRGGKSAI
                     VPLIRRLDRIRGAFRIRLLYGYPAGVTKELLHVMNTSHHVCWYLDLPIQHSHPEILRA
                     MRRGKAVAATQDLAARLRQTVPGLVLRTTCLVGFPGETETHFEHLMEYVERSAFDHLG
                     VFAFSPEEGTPAAAMGGTPSRKVAEERCARLMRLQRRIVRRRVRDLTGREDTVLLLRR
                     TKTGWVGRLPRQAPDVDGETHVSHVPRQAKVGDFVRVEITGGRGYDLMARAV"
     gene            40330..>41742
                     /locus_tag="PK576_01385"
     CDS             40330..>41742
                     /locus_tag="PK576_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE60274.1"
                     /translation="MDTTSFFQDLAVILVAVGIVSAVFMRFGWPKVIGYILAGVVIGK
                     HTLGGSLVINQSSIDVFGQIGVVFLMFTLGLEFSLRKLKKIGHVIFPTALLDMTVMIW
                     AGHFVGTKIFGWGSVQSLFLGAAISDSATTLLAKTINDMGWGTRRFTKYIFGITITED
                     ILCVGVIALLTGLVRSGTMRVGAVAYSMGGLLLFLTGVTVFGLLLVPRVLNRVARLKD
                     DESLLLTMLGFCFLVSFIAAKLNFSLAMGAFLVGVMGAESKPLKRIYQQCMPLRTMFS
                     AIFFVTIGLLVDPAQMLANGPAILGVTLLVICGKSFNCTLGALLTGQDLKNAIQTGIG
                     LAQIGEFAYLVALIGLTMKAVDSSVYQIAVGVSVLTTVLNPFLLRASDPLSDWVVRKL
                     PPRWKEWLETYSNWVDRFQHSSAPSATMKAIRFNLTLVLLHLALVAVVYISAGMLARV
                     DYTRFSPLIEENKKMLLWSAA"
BASE COUNT         7910 a        11471 c        13896 g         8465 t
ORIGIN      
        1 agcccgaccg catgggacgc gcctcgccgc tggagctgga tttcggcgcc atcggtaagg
       61 gctacgcgct ggatgtggca tgccgcatgt tgacggagga ctgggacttt gccgacttcc
      121 tgatgcacag cggcaccagc accgtgatcg cgcgcgggac gatgggcgac ggtcagccgg
      181 gctggccggt gagcgtgggc ggcgattggc gcgaacgcgc cggtttgcac catgtgcggc
      241 ttagcgaggg cgcgatcagc gggtcgggct ttgatgtgaa aggcgcgcac gttgtggacg
      301 tgcgtcgcgg cgtcgcggca tcgcggcatc ctgccgcatg gtcgtacgcc ccggcggcgg
      361 cggtgtcgga tgcgctttcg accgcctgcc tcggactggg atggagagag atttgcgccg
      421 catgcgcggc gttggaagga tgcggtgctt tggtggcgcg ggcgcagccc gtgtggatgg
      481 accggttgag aagtccggtg aggcaatatg gtgcctttcc cgccgtttaa tgaagtgaac
      541 gcttttcggg tctgacgggt cggacaggtc tgacaggttg gacggatcgg acaaatacga
      601 acagaggaac aaggaggaca catgaagcac acaggaatga tgttggcggc tgttgtggcg
      661 ggccttgccg tggtggcggg cgcgcaggac ggagccaagg tgcagttgaa gatcgaactg
      721 ccgaaaccgc agttcacggg tacgcccaag gacattcgct cgccgaacct cgagccggcc
      781 cgcggcggca agccgcgccc cccgatcgat gttccggccg gcgctgacaa agtgctgtcg
      841 cgggactgca aggttacgtc gagcgatcct gagccgatca tcggtgaact gtcgttcatc
      901 actgacggcg acaaagagca cgacgctgcg acgtacgttg aactcggtcc ccaaacgcag
      961 tgggtgcagg tcgatctggg caaggagaag gaaatctacg ccgcgtgcgt gtggcactac
     1021 cacggcgaag cgcgcgtcta tcgcgacgtg atctgccaga tttccaatga tcctgacttc
     1081 atcgatggcg tcgtcacggt cttcaacaac gaccatgaca actcctcgaa gctgggtgag
     1141 ggcaaagaga aagagtacat cgagaccaac gagggccgtc ctttcgccat cagtggcgtg
     1201 aagggccgtt acgtccgctt ctacagcaag ggcaacacct cgaacgagat gaaccactac
     1261 actgagattg aagtgtacgg caagccctga gcgggagtag gagttgggat ttaggattta
     1321 ggatttagga tttaggattt aggagttagg agttaggatt taggatttag gagttgatgt
     1381 ttctgaatcc gaaacctgac ttcggcaaca agagcggcgt ggcgggcaaa cgggtccgtc
     1441 cgccgcctgt tttgttgcgt aagaaattga cgcattgggc cggttcctga tatgttgtcc
     1501 gaaaccatca ggatgggttt aggattcagg aggacgaaac gatgagaatg aggattttcg
     1561 cgacggtatc ggtgatcgga tgcttgtggc tgacggggtg caagcctgcg ggagcgccga
     1621 caacggattc cgtaccggat gcaggatccg gcactgcggc gcaggtgctg gagttgaatg
     1681 acatgacttt tacggcgcag accgcgcagg gcgtggtgct ggttgatttc tgggcaccct
     1741 ggtgcggacc ctgccgtacc caggggccga tcatcgagca ggcggccaag ctgttggggg
     1801 attctgcaaa ggtggctaag gtcaatgtgg atgacgcgca gcaaacggca aagcgttttg
     1861 gggttcaggg cattccgacg ctgatcgtat tcaaggatgg caagccgggc aaacggtttg
     1921 tgggcgtgac tcaggctgcg gtgctggtcg aggcggtgaa agaggcgctg tgatatttag
     1981 gatgtgacgt gctctttccg cctttgcgcc ttcgggattt ttgacctgac agactcccgc
     2041 cggagcccgt atactgcata ccaatgggtt ttatgccgga ggatttgcgg ggggtgtgtg
     2101 cgtgagtgag acggccaagt tgttcctgat cgatgtgatg ccgctgctgt accgcggcca
     2161 tttcgtgttt ctcaaatccc cgcgcgtgac gagtggcggg gtcaacacct ccgcgttgac
     2221 cgggtttgcc aactgtctgt tgcagattct caacgagcac acccccacgc atgttgcgtt
     2281 ggtgctggat tcgtccacgc cgactttccg gcatgaggca tatcctctct acaaggccca
     2341 acggcagaaa atgccggagg acctggcggc cgcgatccct atgtcgatcg agttggccga
     2401 ggcgttgcgg attccgatct tgcgcgtgga cgggtttgag gcggatgacg tgatggggac
     2461 tctggccgcg catgccgcaa cgaaacccgg ctggcagact tatctggcca cgcccgacaa
     2521 ggacgtggcg caactggtcg gcccggcgac gttcctgttc cgtcccggca aggggggggc
     2581 accagcggaa atttacgatg aggcggcggt atgccgacat tggggtgtga gtgcccctag
     2641 ccagatgatc gacctcctag gcttggcggg ggatgcctca gacaacattc cgggcattcc
     2701 tggtgtgggt gagaaaacag cgaccgcact gttggcgcag ttcggcgatt tggaaaacgt
     2761 gcttgcgcat gccgcggaac tcaaggggaa actcgcggag aaagtggcgg ccggggcggc
     2821 gagcgcgcga cagagtcgtg agttggcagc catccggacg gatgtgcccc tgacgatccg
     2881 cctcgacgac ctcgcgtgcc gggatcccga tgaggcgcgt ttgcgcgcgg tgctgaagaa
     2941 atatgagttg gcggcgcttg gcaaacggct gctgggcgag gggtttacgg acggcaccgg
     3001 aggtgttgcg gcatccggcg cctaccaaac gttggccgat gtcccgcata cctatgtctg
     3061 cgtcacggac gaggagcgga tgcgggaatt gctacgggcg ctggaggagg cacccgagtg
     3121 ggcgttcgac acggaaacca cggggttgaa cccgcgtcac gaccggctgg tgggcctctc
     3181 cttcgcgacc gcgccgggac gggcatggta cgtgcccgtg cccgaaacgc atgaggcctg
     3241 ccgcgcggcg ctgcaccgtt tcgcgcccta tttcgatgac ccggctacga tcaagatcgg
     3301 gcataacgcg aaattcgatc tcacggtcct gcgtcaatat ggcatcactc tgcgcggtgg
     3361 attgcatgac accatgctcg cgcattatgt gatcgacgca tcggatcggc acggcatgga
     3421 ccatctcgca cggcagtatc tgcattacaa tccgattccg atcaccgcgc tgatcggcga
     3481 aaagaagcgg ggcacggtgc aggggaacat gggtgaccgg gctcccgagg agatctgcga
     3541 ctatgcggcc gaggatgcgg acatcacgct gcggctggac cggctgttgc gtcaggaggc
     3601 gcaggaaacg ggatgcctgc gcgcgttgac cgagtgcgag gagccgctgg tcggcgtgct
     3661 gttggatatg gagtccgagg ggatccgcat caacgaacag gcattacggg aatacgggcg
     3721 cgaactggat cgcgagatat tggatttgga gatccgtatt cgtgaattcg ggggcggtcc
     3781 cttcaacccc gcttcgccca agcaactcgg cgaggttttg ttcgaccacc tcaagcttga
     3841 tccggacgcc gcgcgcacgg catccggtca gtacgctacc agcgaagaag tgctgctcaa
     3901 gctccgggac cgtcatccga ttgtgccgct gattctggag taccggatgt gcggaaaact
     3961 caaatccaca tatgttgaca aactgcccga ctgcatcgat ccggagaccg gacgcattca
     4021 cacttcgttc tcgcaggctc tgaccgaaac cggccgtctc tcctcctccg atccgaatct
     4081 gcagaacatc cccatccgaa ccgagcgcgg tcagcgcatc cgttccgctt ttgtggcgcg
     4141 cgatgcggat catgtgctgc tctccgccga ctactcgcag atcgagttgc gcgtgatggc
     4201 ggcgctcagt aaggatccgg gaatgatcga ggcgttccgg cgcggcgcgg atattcatcg
     4261 cgagaccgcc tcgcgtgtgt acggcgtgcc ccccgacgag gtcacgccgg agatgcgttc
     4321 cgtctgcaaa atggtcaact tcggcatcat ttacggtatt tccgcgttcg gcttgggtca
     4381 gcggctggat gtgccgcgca aacaggcggc agagctgatc gaaacctatt tcaccacata
     4441 ccccggcgtg aaaacgtaca tggatcaggc gattgccgag gcgcgcgcga aaggatatgc
     4501 gcagaccctc ttggggcggc ggcggttttt gcgtgatatc aattcgcgca acgccacctc
     4561 gcgtcaatcg gccgaacgta acgcgatcaa caccccggtg cagggcacgg cggcggatct
     4621 gatcaagctg gcaatggtgc gggtacaccg cgaattggcc gaacgcggcc tcaagactcg
     4681 gatggtgttg cagatccacg acgaactgct gttcgacgcg ccccgcgggg aagtcgaaac
     4741 ggtccgcgag ctggtcacac aggccatgac cacggtatgg gagatcggtg tgccgctgga
     4801 ggtgagcgtc gggacggggg aaacttggct ggaggctcat tgagcaacgc cttccgggat
     4861 attggcgcag acgcgcggga cgtatgggga atcaggcatg aatgagaagg aagggcagga
     4921 agtgacgcgc aggaacgcgc tggcctggtt gttgcgggtt ggcgcgggcg cggcgataac
     4981 cgccgcggcc gtccaagcca cacggcgggc ttcgggcaag cggacggtct ggcagatcga
     5041 tccgcgcaag tgcattcagt gcgggcgttg cgaaaccgaa tgcgtgctcc acccgtcggc
     5101 ggtgaagtgt gttcacgggt acgcgatgtg cgggtattgc aaactctgtt ttggctattt
     5161 ccaaccggat gccgcgcgtc ttgatgaggc ggcggagaac cagatttgcc cgaccggtgc
     5221 gatccgccgc cagttcattg aatcacccta cttcgagtat cacatcgatg aagcgcgttg
     5281 cacgggctgc gccctctgcg tgaaggggtg cacacatttc ggcaacgggt cgctgtatct
     5341 gcagatccgg catgaccggt gtgtcggatg caacgcctgc cgtatcgcgg aggcgtgtcc
     5401 ggctcaggcc attatgcggg tgccggccga caccccatac ctgctgaaag aaaaactggg
     5461 gccgtccggc ccaaaggaat cggacggatg aagcggccgg ggtgcatccg gaaacgggcg
     5521 ggtctggcgg gtgtgctgtt gccgttggtt acctgggcgg ccgatccgcg ttttccacaa
     5581 ccgcaattcg aatcggggca tacgctgccg cagacgccgc atccggcctt tgtggagcag
     5641 gtgcctgctt ggatggatgt gctgttgctc gcgttggctt tgggcatagc ggtctggctg
     5701 gtgcggcgat tgcggtcgcg acgcggcgta ttgctctttt cgctgctctg cttggggtgg
     5761 tttggttttg tgcggcgcgg atgcgtctgt ccggtcgggg cggtgcaaaa tgtggcagag
     5821 gccatgtgga atggcggcgg gttgccgtgg tttgtggccg caatcttcgt tttgccgctg
     5881 ttcgcgacgc ttctgtatgg ccgggtgttt tgtggcgcgg tatgcccgtt aggcgcgtta
     5941 caggatcttt tcatcatccg gccgcagcgg gtaccccgag ccatggatgc ggtgttgcgg
     6001 ttggtgccgc tcggggtgct tgcgctgggt gtggtggctg cggtcaacgg tgcggggtat
     6061 ctgatctgcc aatgggatcc ctttgtgggg ctgttccggc gcagcgcgcc gatgacggtg
     6121 atgttaaccg ggatggccgt tctgttcgtg gggatggtgg tggcgcgtcc gtactgccgt
     6181 tatttctgcc catacggtgt gctgctggag ttttgcgcgg ggttggcgtg gcgtccggtg
     6241 acgatcccct cgcggacatg tgtcaactgc cggttatgtg tcggcacgtg tccggttggc
     6301 gcggtcacgg caccgcggga ggtgacgtcc ggtgcggaac ggaagcggct tttccggcgg
     6361 tttgtgacgt tggcggtgct gatgccgctc ggtgtgcttt tatgcgcaag tacaggctgg
     6421 ctgaccggtg cttgggtcgc acgggcgcat cctgcggtga ggatggcgga agtgctcgcg
     6481 acggtaccgc aggaggcggc ggatcagcat cccgaactcg aggcgttccg acgcgggggc
     6541 gcgtcgcgtg atgaggctga ggcatacgct gctgaagcag tgcggcgttt ccgagcgggg
     6601 ggggccggtg ccggcgtatt tgtgggggtg gtgatgggcc tgcggctttt ggggatgaca
     6661 cggctgcgac ggcgcccgct gcatgaggcg gaccggatgc ggtgtgttgc ctgcggccgc
     6721 tgttgggacg cgtgccccct gaaccggtct gttgccggaa cctcaggatc gacgtctgac
     6781 ggcccttcag tgggttgagg tcagggccgg ttccggaaaa tccggtagcg gtacgccgat
     6841 acgtggcacg gcactcaaca ggagccgtgt gtacggatgt tccggtgtgt tcagcacctg
     6901 ttcggccggg ccggtttcga cgatccggcc tcggttcatg accgcgatgc ggtcggcgat
     6961 gtgttgtacc acgctgatgt catgcgtgat gaacaggtag gagagtccgt gtttcgcctt
     7021 cagatcttcg aggaggttca acacttgtgc ccgcaccgaa agatccaacg cgctgaccgc
     7081 ctcgtcgcag atgagcagtt gtggcccgag ggcgagtgcc cgggcgatgc acagacgctg
     7141 gcgctgcccg ccggaaaatt catgcggata acggtcgagg atttcttccg gcaggccgac
     7201 atcagcgagc aggcgtaccg ccgcgtcgcg acgggtttca ggcgtcacca gccggtgcgc
     7261 caacatggct tcggtgagca ggtcgagcac ggtgtgacgt gggttgaggg aggcgtatgg
     7321 atcttggaac acgacttgca cggtgcggcg gtaggcgcgg agatccgcgc cgcgaagcgt
     7381 ccggatgtcc cggccgttca gcaggagggt cccggcccgc ggtttctcca ggcgcaggat
     7441 agtacgcgcg agagtcgatt tgccgcaacc cgattctccg acgatgccca gagtctcacc
     7501 ccgcatcaga tccagatcca caccgtccac cgcgcggata tgcccgacgg tacgggcgaa
     7561 gacccctttt ttgacggggt accaagtttt cagcccccga atccgcagga gcggcatatc
     7621 cctgtccgaa ttggccatgc cggtatatag cataccggca tggcggacgg acaggataaa
     7681 aggcgaatgt cggtgaaacc cgcagcggat tatgaggatt gggagacaaa cgaaccgggg
     7741 tgaccagaaa ctccggtttc cttccgggaa gcggatgtgt tatgcttctt tttttcaaac
     7801 ccaatgagga atagccggac atgaacaaga agacgttgcg tgatgtggac ctgaagggca
     7861 agcgcgtggt gatgcgcgtg gatttcaacg tgccgatcaa ggaaggcgtg atcaaggacg
     7921 atacccgtat tcggggtgcc ttggcgtcga tccgttacgt gctggagcag ggcgggagtt
     7981 tggtgctgat gagccacctc ggccggccta agggcaaagg gtatgaggag gagttctcgc
     8041 tgaagccggt ggctgaacac cttgccgggc ttttgggtaa gcccgtgact ttcgcgccgg
     8101 attgcgccaa cgccgacgcg gaagtctcgg cgttgcagcc cgggggcgtc gtgatgctgg
     8161 agaacacgcg tttttacaaa gccgaggacg gcaaggtcaa aaagaccgat gagatgagcg
     8221 atgaggaggt tgcccggaag aaagccgagt tgaaagccaa gcgcgaggag ctggccaaaa
     8281 aactcgcctc ctacggtgac ctcttctgca acgacgcctt cggtaccgcc caccgcgccc
     8341 atgcctccac agccgtgatc tgtaagttca ttccggagag tgtggcgggt ttcttgatgg
     8401 agaaggagat cgagtattta ggcaacgcgg ttgagaagcc gacccgtccg tttgtggcga
     8461 ttctcggcgg ggccaaagtt tcagacaagc tggcggtggt caagaacttg ctggataagg
     8521 tcgacacgct gatcatcggc ggcggcatgg cctacacgtt cctgaaggcg caaggccacg
     8581 atgtcggcgg ttcgctgtgc gaactcgatc aattggccta tgccaaccag atgatcgaga
     8641 aagcgaaggc caacggcaaa cggctcttgc tgccggtgga caacgtcgtg gccgataagt
     8701 ttgatgccga ggccaatacg caggttgtcg gcaatgacat tccgtcgggc tggatggcgc
     8761 tggatatcgg tcctcagacc acggagctgt atgcgaccgc gatcaagggt gccaagactg
     8821 tggtctggaa cggtcccatg ggctgtttcg agatggccaa gtttgcgggc ggcaccaccg
     8881 gcgtttgtaa agccgtggcg gagagcggcg cggtttcgat catcggcggt ggcgacagcg
     8941 tgagcgcggt caacaagagc ggtctggccg ataagatgac gcacatctcc acgggtggcg
     9001 gtgcttcgct cgaattcctc gaggggaagg aactgcccgg tgtggcggcg ttgagcgaca
     9061 agtaagacgc ttcaggtgtt gaaagggaag cagccggacg cgggaatcac gatcggctgc
     9121 ttttttttgt tccgataagt ttgtcagact ttggttccag cggaagaagc tgattccgca
     9181 ggtgcctgtc agtaatgcgt tagcggaaac cgttttacgg agcgggaggc gtcaaggcgg
     9241 atatcgtacg ctcggagaag atcacgcgcc aggaaacctg tccgccgctg ccgctgatga
     9301 atgcgaggcg tacgaggccc tcttcggatt tcggttcgaa gagtgtggtg cggggggcgt
     9361 ccggtgtgtt ggcgtgggcg taggcgtcat ccggacgggt gacttggcct tcaccgggcg
     9421 cgatcgtcag ttcatatcca ttcggaccgg tgatcgctgc ggaaagtact gtgccgaagg
     9481 atgctgaggc gtccgcatgc gtgaggatgc gcaattcgta tcggtcatcg gccacggtgt
     9541 cactgacgcc ggagagcgtg cgcgagaccg gatcccaagt tacggcgcgc acatccgtaa
     9601 tgccctgcgt gatgtgtcgc gaggtgctga tgagttgagg ccggtcacgt atcggacaca
     9661 gcgcgaggac cgcgctggat tgcggcggga gggtgagcgg tagactgtgc cggaaggggg
     9721 gcgtgagggt gttgccccag tattcgaacc cgatccaggg ggaacgggtt tcgagaccga
     9781 gtgaggcgag cggcacgttg aaggttttgt ttgtgccgtc ccagttaaag agcccggcga
     9841 cctgacgttg cggccgttcc gctgtcgcgc catctgtcac cagccagatg ccggggatcc
     9901 ggttttcgaa gagatcggca ggccgggcta cggcctgatg cgagggcatg gtgcgccgga
     9961 gcagatccag acggtccggc ggcaaccccg catagtgttc gctgctgctg ttcatctggc
    10021 cgctcagagt gacccacgcg gcaagggctt gcgcttggtg gatcggcaag gatggcctca
    10081 catacagcgg gtccggatcg ttataccaca cgcggccgtg cagaaagtag ttccatgcgc
    10141 cgtattctgg accgcgcatc atggcggacc aattggcccc gttgtccggc ccgatgcgca
    10201 tcgcatcgac cagaccgaag gccgcgcccg cgctgcgcat gttttgcggt gtgcagcagc
    10261 cgagcaggaa cacatcgcgg cctgcggtgt cgcgcacgat tttgagactg ttacgcatca
    10321 tctggatcgg ggtgacggag gggtcggtga gtatcgcctg accgatctca tcctcgcgga
    10381 aggtattgca cacgtaattc aggttgatcg ccgcgccggc gtagagcgcg tccattttca
    10441 gataacggta accccactcc gcagtgaggg tgcggatcat gtcgcgcaga taagcctgcg
    10501 tatcgcggcg ggtcaggtcg aaacaagtcc cgccccaacg ggtgtcaaag ggcgatccgt
    10561 ctgatcgttt ggcgaaccac gccgtcttgt cagcgaacca ggggtcttcg cgtgaacctg
    10621 cgaaggggat cagccagatt ccggcggtca tcccggcggc gcggatggtg tcggacatgg
    10681 ctttcatgcc tgcgggataa ggaccgtcgg ggcgatggcg gatgaagact ttcttggggc
    10741 cgttggtcga ttgtccgctc tgccacccgt catcgatctg catcacgcgg aggccgaacg
    10801 gcttgagcgt ttgtgccgca tacgcggtcc ggtcggtcat tacccgttcg ttggaggccc
    10861 tgtccacata ccaggtacag tggacggtcg gcatgggcgg caggcggatc gcatagatcc
    10921 gggcgatggc gtccgcataa gcctcgagac cgaaacgcgc gtcgtcaaac aacccgaggg
    10981 cgaaaagttc cagatcttct tcgcgatctg cattgggtgc gaggcgaccg tagtcgagat
    11041 gcggcaacag cgtgacgcat tcctccgcaa cagccatgcg cacgataccg ctgccgcggt
    11101 ctgttgtgag ccagcccgca accacgccac gccgcgtggc gggatcggcc acggcagtcc
    11161 acatgtagct gccgggattg cgtttcggct ggagcagccc tccggtaccg aggctggtca
    11221 gttcttcggc gggccggtcc agcgcgagcc gcaccgcagg gtaggcgatt cgattaaccg
    11281 ggttcgtcgt gttcgtgccg cgccaagatg tgcggagcac ggcaaaaggc agatccgcaa
    11341 agagcatcac tcggttgcgg ctcccgtggg ggaagtcgaa ttccaacgcc tcaccagcac
    11401 ccagacgggg ctcgttcaga cggaccgtac ggacggcaga cggtttctgc gtagcggcat
    11461 cgaatcgggc aaagggtttg gactcgccgg gaacccacaa ggtcacctcg ccggaagcct
    11521 cccggtattg gagaagaacg gcgcggttct gaagggtcag atcggtggcg gaaaccaatc
    11581 ctgcggcaac cagcagcagg catctgagta atgttggtcg ggaggtcgtc attcgggtcc
    11641 tttccggcat ggagaatcac atgcttcgca gggtaacaga ggcgcgggac cgattcaagt
    11701 tcatgcggct gcgcacagcg cgacggcttg gcgtctctct catgcaaagt acctgtttgc
    11761 gcgtgtcgga tcaagcccgt atccggataa gcgtaacatc tgccgtgcgg ttgtttgcga
    11821 tcccccgcca tcaacgattg cgcaaactgc gctttacgga cgggctccga tggggtatgg
    11881 tgaaattccg gttgaatatc ggatcacaaa ctacaagggg gaaccatgag gctaacggga
    11941 aggggattgg ggacgatgtg gttggcggcg gtgtgtgcgg cggcggggat tgtgtcggcc
    12001 gcgccggaat accggcaggt ggcggcagag gaggtccggc cgcgcggcgg aatcggtaat
    12061 gtgatgcgga agatccgtgc gggcggtgag gtgaccgtcg cctatctcgg cggttcgatc
    12121 acctcgatgc agggttggcg caacaaaacc accgattggc tcaaggcgac gtacccgcag
    12181 gcgacgttcc gtgaggtgca tgcggcgatc ggcggcaccg gaagcgatct gggtgtgttt
    12241 cggttgggtc gcgatgtgct ggcgcagaag cccgacctgc tctttgtgga gtttgcgacc
    12301 aatgacgggg ggcaggcgcc ggagcagatc tggcgcagca tggaagggat cgtgcgacag
    12361 acgtggaagc atgatccgct gacggacatc gtcttcacct acacgatcac gacggccatg
    12421 acggaggact atctgaaggg gatgtgcaac cgcgcggcct cagcgatgga gctgcttgcg
    12481 gaccactata atatcccgtc gatcaatttc ggcgtgcgcg tggcacagtt gctgaaagag
    12541 aacaagttga ttatgagcgc ccgggagatc gaaaccgctg tgccgaaaga gtcgccggat
    12601 cgcgaccggc tgatccgcga acggatcgcc gccgaccccc gtatgctctt cgcgaacgac
    12661 ggggtgcatc cgcgggacga ggggcacgag ttgtacaagg ccgcgatcat cgccggcctg
    12721 acccagatgc gcgacctgcc ggcggtcgac cacaaggcta gtctggcgaa tgtgtttgta
    12781 caggaccatt gggaggcggc taaaatggtt ccgatccggg catccatgct caacggtaac
    12841 tggaaggcac tgccggagga cgatcccaag cagaaaagtt tcgggaagcg tatgggacaa
    12901 atctggatgg ccgatcagcc gggcagcacc ttgcggttca agttcaaggg caccgtggcc
    12961 aaggtgtacg atctgctcgg gccggacggt ggtcaggtca cggtttccgt ggacggccag
    13021 accggtgcca agcctgtgcc gcgttttgat agctattgca cctaccaccg gatcgcgaca
    13081 ctcggagtct tcaccgggag cggtgccgag gtgacacgcg aggcggtcat cacgctacat
    13141 cctgaacagc ccgaccgctc cagcatctcg ttccggctta aggacccggc caaagagctg
    13201 gctgcgccga aatttcaggg cacccggatc tggatgagcc aaatcatgct gatcggcgat
    13261 ctggtcgagt aacgcaatcg cactgccagc tttgcgcaat gtcaggtggc cgccgcagtg
    13321 agttcatccg aacgctgggc gatgttctcg aggtggcgca cgatattttc gagtgcgccc
    13381 acgacctcga cgtaaaccag accgcgttcc ggtgtgcaga ggcctttctg cagacgttcc
    13441 acatgccctt gtatggactg gcgcgccata gccttcaggt cgttgaccac gacctccacg
    13501 gctttagcca gaaggcgtcc gtcctgcagg ctttcccgtg tgagattggc gatgacagac
    13561 gctttgtcga acaaggcttc cagttcctgc agcgccgtgt ccgaaaactt cgtgttttcc
    13621 gaagtcagcg aggccgagcg tttcgcgatg agcagggcga ggtcggagat ccgttcagcg
    13681 tcgttcacgc agtgcatgag cgcggggatg gcggccgcct gtttctccga aagatccttg
    13741 cgggtgagtt gcaccagata atccatgatg ccggtctggc aggcgtcgac ctcatcctcg
    13801 atctcctgga tgctgttcac gtcaacaggt ttgccatcct tgtaaccgcg caaagaccgg
    13861 gcggccgcgt tccaggcggt ttcggtcata tcacccaagg cgcgcatggc gcagaagagc
    13921 gcgagcgggg gcgagctgag caggtttttc tccagatgaa cgacacggga ggtcactttc
    13981 ttacccggca tgattttttc gcagagccag gccaatgcgc caatgaaggg ggtcatgagg
    14041 atcacattcg tgacattgaa cagcgtatgg gccatggcca cgtgacggcc gatgttttca
    14101 cccaggaaga cattgcctgc ggtcacgctg ttgaccaggt acaggaaaca gggcacgttg
    14161 tggaaaggga cgtaaaagag gccgaccatt acgcaggttc ccagcacatt gaacagcgaa
    14221 tgggctacgg cggtctgccg ggcggtgcgg ttggcgttga ttgaggcgag cacggcggtg
    14281 atggttgtgc cgatgttgtc tcccaaaacg atgggcaccg cagtccagat gttcaacagg
    14341 ccgctgttgg ccagcgcgat ggctagaccg attgtggccg aactgctctg caccagcatc
    14401 gtcatcagag tgccgatccc gatcgcgccc agcaccgcgc cgatcggcat cgtttgacct
    14461 gattggggtg cgcagtcgaa gatatggaaa aagcggacga aactcgggaa ggtgctgacg
    14521 cttttgagtt catccgacat gagggtcatg ccgtaaaaaa ggaggccgaa gcccaggatg
    14581 gtacgtgcgg ttccctgaag tgtcgcttta cggcagagca gcaggccggt gacgcctaga
    14641 atgatggcag gaaaggccag gttgtcgagg ttgaacgaga ccatctgtcc ggtcacggtg
    14701 gtgccgatgt tcgcgccgaa gaccacgccg atagcctgtt tgagcgagag cagccccgca
    14761 ttgacgaacc ccacggtcat gacggtcgtg gcgctggagg attggatcag ggcggtgatg
    14821 aaggtccctg cgaagatcgc cgccactcgg ttacgggtga catacgccaa tacggccttc
    14881 atccggtgcc ctgcaatttg ctgcaacccg tcgctcatca gggtcatgcc gaaaatgaag
    14941 agcgccagtc cgcccagcac gccgatcagc gtgcggaaca ggcatatgga catggcctga
    15001 aggaacaccg gccgcgtgaa caatccgcgt ttcggatccg ccacctcggc ccggacctcg
    15061 taggtccccg tggcgtgggg gtcggtcttg agggagacct cggcgatccc gtcgggatcg
    15121 gtccgggcaa acgaggtccc gatacggccg tgttttcccg gttgttgggt cagggtaaag
    15181 tgaacccccg catcggcgag cggtttgccg tctggcgcga caaggcgtac acgcagaggt
    15241 ttcggcaagg gatcaccggc ggagacctcc tgacggacgt tctctaccgc gacgccgcaa
    15301 acgaaccgca gccgtttacg gatttcgggc gcctcttcgc agaccaagtc cagataatgg
    15361 tctccgaaca cggctccgaa cgtcacgtcg aatttgcacg atccgcccgc atcggtaagt
    15421 tgcgagggag gggagacgcg cacaccgccc gcagggtcga ccgggatcgc gttgatccgc
    15481 ttgcctgcca cagggtgttg tgtgcccgcg ccgcccaata ggcttgcgac gggtttgctc
    15541 agcagttcca catggatggc gttcaccata ttggtgccgg gcattccaag cagggtttcc
    15601 gacgagaata agaccagctt ctccggttcc aagccttttg gaacccggtc gcatccggtg
    15661 aggaccggga gcgtaagggc gagacacagc aggtagcgca cgaaggtttg tgttttcata
    15721 agtgtgaaaa aggaaaggcc tctcttattc ttgtgttctc ccgttgagtt gaaacagaca
    15781 ggaggactca cacgttatcg caggatcagg catttcagga gagacggatc ttgccgacca
    15841 gtcgcatcag gagcgctgtg gcaacgaccg tgaccaccag caggaaactc ccggcgaaca
    15901 tgcgtccgta gagcaggttg ccgacgccga agagcgcgga ccagaccgcg aggcaaccga
    15961 tcatcataca aagcaggccg gtcgggacat cccagccctc tgcggatttc ggcagcgatg
    16021 aggcctcttc gatatggcgc cagccggggc cgcccgcgcg gatccgggta cagaaccggg
    16081 ccagcagatc ctggttttcg ggaggggtca gataggtcac ggtgagccag cctatggtgg
    16141 tgatcaggat gccgatcacc agtttccagg cgctgtaatc catgacggca aagagtccgg
    16201 cacgttccat ggccgcatcc aatccaagcg gcgcggcacc ccattgaaag aaacaggcca
    16261 ccgcgaacga aatcaccatg gcggagatct cgctccaggc attgatgcgc caccagaacc
    16321 agcgcaggat atagagcagc ccggtcccgg caccgatttg taacagaatg tcgaaggagt
    16381 cttttgcatt gcgcatccag agcgcgacga gtccggagag cacgaggagt gcgagcatgg
    16441 tgacacggcc catggctacc gcctgccgcg gcgtactgtt ggggttgatg aaccgggagt
    16501 agaagtcttg caccacatac gaggaacccc agttcaggtg tgtggcgatg gtggacatgt
    16561 aggcggcgat cagcgaggcc acgatcaggc ccagccaccc tttgggcatg gtggagatca
    16621 tccccggata ggcgatgtcg tggcgcagga actgatcgtc gactttaggg aacgcccggg
    16681 cgagactgcc cacccctgcg gcatccgcct tggcctgccg cacttgttcc cgctcgggtt
    16741 ccggcatctg cattttcgcg gtgtcatatt gtccgacctg tgccggatgg tctgcgagcc
    16801 atcgcgttgc ggccgcctgt tccgccggag gcgtgagcgg gaagtgcaca agcgagacga
    16861 gagccacgat gatccaaggc caggggcgta ccgcatagtg caggaaattg aacaacaggg
    16921 tcgctcccac cgcgtttttc tcatccttgg ccgagagcat acgctgcgcg atgtatccgc
    16981 cgccgccggg ctccgcgccg ggataccaga cattccacca ctgcaccgcg atcggcagaa
    17041 tcagcacggt catcagggcc gaaagcccgc cctctccgga cgggaacatc gacatgtgcg
    17101 tctgcacctc cggttgggag aagagctgct tcagcgaagt gatgcctcct acgtcggcgg
    17161 atttgacggc gtaaactgcg gcaaaaatcg cgccgaccat ggcgatcgaa tactggtaga
    17221 aatcggccca gatcgagccg gtgagtccgc ctagggtcgc atagattccc acgccggccg
    17281 aggcatagag cagggtggcg accggtgaga ggccgaacat cacctgtccg atcttgatgg
    17341 ctgccagcga gaccgtggcc atgatcatga tgttgaagaa aacacccaga tacagggcgc
    17401 ggaacccgcg cagcagggct gcgggtctgc cgctgtaccg gatctcgtaa aacccgagat
    17461 cggtgtccag cgcggaacgg cgccagagtt tagcgtagac gaagaccgtc agcatgccgg
    17521 tcagcagaaa ggcccaccag gcccagttgc ccgcgacacc ctgtgtccgc acgatgtcgg
    17581 tcacgagatt cggcgtgtcg cacgagaaag tacaggccac catggagacg cccagcagcc
    17641 accaaggcat ggaacgcccg gagaggaaaa agtcggctgc gctccggctc gaacgacgcg
    17701 ctgcggcaga gccgatggcg atcacagaaa agaggaagcc gaagatgatg acggcgtcca
    17761 gagtcgagat tttcaacatg ttttttttcc tttcacactc cggttacccc ggaaagcata
    17821 tcccgtcttc ccgacggttc ggtcatgacg catccggcgg aatcaggtcc gcgactttca
    17881 ctcgaaccgc ctcgcgctcg cgttccagcg tccgcaacgt ggggtcatcc gccggaacga
    17941 cggcgggatg ggtttgattc cacttcgtaa aggctgctga ggttcgggtc acctcgcttt
    18001 ggaggcgaac ctgctcgtac ttgagcgcgc gcagggcgtc cagaactttg accttgcgtg
    18061 cgccggtgca tgtctgcgcc tctgcttcaa gccggtcggc ttgggcaagg ctctgctgat
    18121 aggcgcgttg cgcgtcacgc gcggcaagga agtggggatt ggccgcatgc gccttttccg
    18181 ccagcgtcgc tttatacgcg gcgattttgg cggtgagcag gaaaaaggag tgtagattcc
    18241 cgacaatctc gggatccacg gtttcggggt ctccctcata ggtggcggtt tccgtgcagt
    18301 cgagcaggca gggttcgtcc caggcattgt tgcggcgcac ccggctcaac agcatcgggt
    18361 tcttggacga ggctttcaca tcggaataac ggaacagcgt gccgccgggc atggtgccga
    18421 gacgcttgcc ctccggcgaa tagtacgaca cttcgcgttt cgtgaagccc cagccgcttt
    18481 tcggccccat aaacaccacg cgtgtgacac ggttctgcgg ggcggcggtt tgcgtcaagg
    18541 ttttgtcagc ggaagcggtc tgcccgctgc ggttggatcg ccgctgtgcg ggctgcccag
    18601 tcaaccgttg cgggttccgg gcggaacgtt tcacctctgc cgttccggac agggttattc
    18661 caatcaggca gaggcagaac cagacgttgt tcggaagaag tcgcatagca ggccgatcaa
    18721 acactgttaa agagtgaatc atattcggcc ccatgttcca agcaggtttt tcgtcggatg
    18781 aatcgattac gggaaaacag ttgccgcggt tgatggatac gcgtatggta atcccatgtt
    18841 gcggcaaaac aagaggccgt gcccggagac gcgttttgcg cgcggggtac gggcattgcg
    18901 gaaactcacc ccgggactga ccgaccggga acgcgcggct ttgtggctgg tgcttgccct
    18961 gttcgtcttg ggagcacttg tgcgctggct ccgcatgcaa gacggttgag ggaggcgtgt
    19021 gaaccctgcc gtgtcacgat gcgccccctg atgaggcagg gggcgcgtat cggactaagg
    19081 ttccggcggc gtgttcacca gcggcaggac tccgatattg gggtgctgtt cttcgattac
    19141 ccaacggaag aaggcttgag ccggatccca gtacgggaaa ctgtagatat acgggttggg
    19201 cagcagaatc cggcactgat ggttcgtgtc gaacgaatcc ggcatcagcc tgtattgtcc
    19261 ggcgatccgc caatcgggga ttgtcagcga cggcgtcatt tccagtttca tgaccgcctg
    19321 gctcaacaga caggtacagt tctgcccgtt gacggccagc gaggccgtaa cataataatt
    19381 ggtttcggca tcacgcgtga aacggacaat actgccgtcc acacggttgg ttacggtcgg
    19441 atccgcattg agccaataca gttcggtgag ggacatcaac ctcccgttgc cgaagaaata
    19501 actcgatgca agcggagtgt tattatcgcc gaacccgagc agccagttca gcatatcgtc
    19561 gctgatttcg agatcagcga tatcctgacg cagggcgaaa tgagcttcca cattcaggtt
    19621 ggcagagaca ttggtgagcg gtaacagata gttttgcaag ccttctccgg gcggaagttg
    19681 ttccacctga ttgaccttga cggaatagag acggtaccag ttgtcagcct gatacagaat
    19741 gttggtgttc gcgccgttcc gcgtacggaa ggtgtggcac tgcgtgcgca aaccgttttg
    19801 cgtaccgttc aacgcattga gtgtggacac gatccagtgg ttcgagacgc ccagttcggg
    19861 gaaggcgaca gactgaccgg ggttcagccc gccgggggtc caatagaggg ggaaggtcca
    19921 gtccgcgcgt gtgacaccgg tgccgttggt gacattgagt gaaccgccat gggtctccgg
    19981 tccgacatac acgaactgcc tttccggatc ttcggccgcc cagagttcgc catcgaattc
    20041 cggtccggag ccctggctcg ggtcatcgta tgcgatgaca tgctcgtaca tgcccatcgg
    20101 tcgtttcagg cgcaacccgc ccgcgtagat acaagggatg taccagtaaa agccgggctc
    20161 cccgtaatcg agcttcggta acggcaccgg tgttctattg ggatccggaa atttctcgcc
    20221 gatcacgaag aacgcgtagc cattggtcac cgccgtttgt tcgggcagtc cctcgggcag
    20281 ggtgatggta tgcatatccc tgtcattcat gaccaggtcg atactccagc ccgcgagatc
    20341 gacaccgacg ggcacggcga tttcgatgta cggattgtca taaaatccat attggggcac
    20401 accgttctcc tcgacatgtt ccgccgcgtt tacctcgttg atccacaccg ttttgagggg
    20461 tacgccgtac acgatgtaat agggggacca cccctgcgaa gcgtaaaccg cgttgaaatc
    20521 cactgggaag taccaaggag gattctcaaa cgttttttgt tccgcagtca agggtatccc
    20581 tcccagatag gtggcgcgca cacggtactg cacgatggtg tcgctgtcca gcatgggaag
    20641 gtcctgagag gggagggtct gataggttgt cgagttgccc atttgctcca tcggcaacgt
    20701 caccacctgc gccgcaggcc agttctcgac gccccagaca ttggtgccga tatagtaatc
    20761 gacatacatc tggatgttcg aaggcgagag ccggatgttc accaactccg cctgaatgcc
    20821 cacgtggtcg gagttgatag gctggtggcg gggcgggtca tcatcatacg aatcgccgtt
    20881 gcggcagagc acctgcacat tctcgatgtc gaaacccgga aagactggtt cggtcaccgc
    20941 gatatcctca atacaggcac gctggccgcc cagcggggaa atgaatttga tcgcatcgta
    21001 atcgcgtccg tcaaccggtt ttacggtgac cgtttcataa aaccggttcg tgatctgggt
    21061 gaaggcggcg atctcgaacc agaggtttgt gggcgcattc catccgttgg tggtcgcgta
    21121 aatgtaaagg ctcgacggaa aagtgttggt gtaggcgcgt gtcttgaagg agagttcccc
    21181 caaaccgtac ggcagaaccg gggattgcac gaacggcacg ttctgatcct gaatgggctc
    21241 cgcatcctgc gtggtgctgt tgttcagcaa acaggcttcc tcgccatcca acatgatccg
    21301 ctgtttgtcg gtttgggtca cctttacatt atacgcgcgc catgaggtgt ccttaacgac
    21361 aggctcttcc caagcccgga cattgtcgat ttcgacccag gcgttggaac acccgccgct
    21421 tcggtcgttg atcacccgca ggaatcccgg attggtaatg cccacgtatc ccgagatgtg
    21481 gtcccactcg gacatcacgt tgctgatggc gtaggtgtgc acggggatcc agatgtcgtt
    21541 ctcgcgctcc gtcgcgtact gcacggtgag ctgtgcggga gcgcgcagca cacggtaatc
    21601 gaactccagc atccccatgc cgttttcgag gaggatcgag cggaccgcct gatccgcatt
    21661 cggatcggcg cgcgtgtgtt tgagctgtat cacatggccg ttcgccgcat tgttggagac
    21721 ggcccaagcc tcggtggcga gccaggcgtc gtcattgccg ctaccgaact gttcaccgcg
    21781 ccaggaggtt atcttcaact catccaccac gacatcggct ttcgatcccg ccactcccat
    21841 gacctgcaac aaaaggaact gtgtcttcca atccttaacc gttacggcat tggtcttgta
    21901 tccgtacccc gaaacggtga tctcctggaa aagcttccat gcggtcgtgc ccggcgcggc
    21961 gggctcggcg gttgagtcct gcgcggagct ctggaggtaa accccgagtt tctgggtggg
    22021 gatgaccgcg tggatcccct tcggcgacct atctccatca agaacaaaac ggccccaggg
    22081 ggcactccag ttgtttttat ccgtgttgaa cgcgttcgta tccgacagga cggttgtggc
    22141 acttccggtg agggtcgctc cggttgtggt cggttggata atgaaattcg agaaacctgc
    22201 accgcaatcc tcggagagca cgccgtaggt gccgtaggtg aggcgggaat cggagttgtc
    22261 ggtatgatcg atccgtaata cagcgcttga tgacggataa tagtaacagc ggatcagcgt
    22321 actgttgggt tggttgaata tacggatttc catagcgtgt tcgaatcccg ctatatcccc
    22381 cgcgttaccg gtgtcatacg tgcctgaggt tgcaagcagg gtgggcgtgc cgttgatcca
    22441 gcggtacaat tgatgtgtca cccgcttgtc gcgtgacaga ttatcggtgt cgggaacttg
    22501 agtgatgcgg tattcgtaaa aatggtcggc atcctgataa taggcgatca cggagagcga
    22561 cggggcgtcc ggtgagtgtc ccgtgtagga acgggccgtc actttggccg aatagttcga
    22621 actgatgaac gcggtacggt cgtacgcgac gtcgattttg tcggcagtct gcgccagacg
    22681 gtatttgaag gcgaaatccc ggatgccgtc ggtccgggtt tgcaccgtat tgtggacgta
    22741 ccccaagccg agggtacagc cacccccttg gagacgcagc gcctgattgc ggaagttgac
    22801 ataccctgcc ggcttgttgt tctcgtccgc cagcgttcgt tcgacactga aggccgcgtg
    22861 accggccatc cagttggcct ggggggtgcc gaacggctcg cggacaaaga cgttcgtggt
    22921 ggggacgggc agatggaagt attgggaata ggtgtcctcc acactgactg gccacgcctc
    22981 aaaggtgttg agaaaactcg ctttgggggt ttgcccgtta ctctccgaaa acttgtcgga
    23041 gagggccggc caattgttga aattctggta ctcggcacgg acgagcatgt atttcaaggt
    23101 ttccgtgtcg aaagtcagca tcacatacgc gccccgatcc gccgtgaccg tcttggtgag
    23161 tggcgtttcg gtttcctcgc agaccccgcc gtaaggcagg cggttgatgg tgccggggag
    23221 ggtttccgcc cagtggacaa ccgaggtcga ataggcgacc tcgaccggca ggtagcggcg
    23281 ttcccctttt aaacgccagg agacattggt tgtgcccgcg tcgctgagcg gcagcatgcc
    23341 ctgccaagtg ttgtccgcca caagttccat ggtgaccggt tccggatact cgtccgcgac
    23401 tacggagatc gaaccatagg gcgagacgaa agggcggatt cggatggaga acggttggga
    23461 acggcttccg agcaggcggg gcgagaaacc tgcggccgcg gcttctgaag gatagacatg
    23521 gccttcaccc gtgtaatcgg gcgactggta actgtagccc gcatacgagc actcgaaata
    23581 gtactccaag tcaccggtcc gcgggtgggg cgggatcacg ccttcatacc cctcgttatt
    23641 cccctggatg ccctgaaaga aattggtgag ggtgacactc gtccagtcat tgctggcctg
    23701 atccagataa cgccagcggt aatagacttt gtttgtgcgg ctggtccagt cggtgggcgt
    23761 gttcatgtcc acattgtccg tgaaacagcg gaccgtcatg ttcgtgccga ctgaggggta
    23821 tcccggctga aaagggcagg cggttttccg gaccacgaca tccgatggcg gttgcgagac
    23881 ccggatgttg tcgatgacgg tgaagaagga atcgagacca tctccaggcg catccgtgta
    23941 aacacccccg taattttccg cttcaggagg gggaagcgcg gagccgcccg aaaccccgcc
    24001 gctgcgctgc cagcgaagtt tggcgggacc ccggtagttc agggagcgta cataacggta
    24061 aaactctccg gaattcgagt aatccaaaac aacatcatcg ccaaattgta cccagttata
    24121 ggtgaggtct tgggtttccg cgtcaagcag gatgttggtg ggccagccgc tggtttggtc
    24181 agtcatgttg gtggtcagcc agatcgtgat gcgggtgggc cgcccggctt ggttgttcac
    24241 cgcttcaaaa taaacggtgc cgatcccttc ttggaaaaca ggcgagatga tcgccgcatc
    24301 caacgtgttt tgcaggttgg cggcgaaatt ggtggcggtg ttaatgttga aagaaggcaa
    24361 tgtggggggg acgatcagcc cggatccttg tccgcgggca gaaccagtgc gcgtgttaaa
    24421 aatattccat ccagcggaat gaaaatggtt tgtgcctgag cgtaaggctc ggcgagtagc
    24481 gagccctgta attaccggcc cctcatgcgt ccaaagcgag gtgtccgcat aatcatcaaa
    24541 cagcaggata cgcgctaccg gcagcaggtt ggtcacgccg gacgcgacac ctgtgcaggt
    24601 caagaaaacc gccagcgcgg cgcacaaatc gttactcact cgtttcattc cgatgctcca
    24661 cacacgtctg tccccgtccg caaaaaatgg ccgctcccaa cgcgctggtt cttgctcacc
    24721 gcatccggtc cctcaactct tcggtgatat cctcgccatt gcagagttgc cttgtcagtt
    24781 cgatgcagtc atccttgttc cccccttcgg gttcgatcaa cgcatcgaca tggccatcgg
    24841 cataggccac atgcgccacg taccgtttgc cgactttgtg gttgaatccg atcatctccg
    24901 gtgacccggg attcgcatag tttttgatct ccgcctgcag cacgctgtcg gcaccctccc
    24961 ccgaagagac gatcgacctc gcgggaagtt ccgcgaagag caaccggctg gccgcattgc
    25021 cgcttgagga gagactttcc agccagcgcc ggacatcggc ggttttcggg ggggctttat
    25081 gctcgtaacc gaaataggcg ttcattacat aagagcggtg gattttctcg agaccggttc
    25141 gtgcggcggc tttcttgtgc gtgtcgcaaa ggaaagtgga gatgtctttg cccacaaacg
    25201 tccacaaact gccgttcgtg agcgagtagt acgcattgtt cccgtgaaaa cacgagatct
    25261 ccatgttttg gatatctttg ttcggccaac ttccggatcc gacccagcct acccagccgc
    25321 ggtgctcgtg gtatttcggg acgtaacggt tgccatcccg gctggggtaa cgcacctgga
    25381 acgaccctgc ccaaggcatg tgtttggtct ccacggcata actcagggaa gcctgcgcga
    25441 gattgcgcag gttggtcttg caccgaatcg cctgtcccat ggaccgtgcc cggcccacct
    25501 gtgtcgtcag ggcagcggcc agaatgccca gaatggaaat caccaccagc agctcgatga
    25561 gcgtgaatcc ggtttttcgc acaaaaagca taaccctcct gtgtcttttt ttatcagttt
    25621 gatattgtcg ctgttatcgc ctcaagtggc aaggcctatt ctcactcttt tttgacttgc
    25681 gaatcatcgg aaaatacggt gaaataaatc gcaatgtttt cgtaaagcgg gatttaagaa
    25741 ggataaaagc gaaatgcgtt ggtcgcgtgc gttgctcaat accctgcggg atgatccgca
    25801 ggaggctgaa atcaacagtc ataaacttat ggtccgcgcc ggcctgatcc ggaaggttgc
    25861 gggagggttg tatacgtttt tgcctctcgg actgcgggca ttgcacaagg tgacccggat
    25921 cattcgggaa gagatggatc gggccggcgc tcaggagatc ctgatgcccg cgctgcagcc
    25981 ggccgagctt tgggagcgtt cgggaaggct cgccgccatg ggaccgggga tgttccgtct
    26041 gaaagaccgt gcggagcgtc tgatgacgct tggccccacg catgaggagg tggtcaccag
    26101 tctgttggcg ggtgaagtca gttcctaccg tcaactgccg tgcacggttt atcaggtcca
    26161 gaccaaattc cgtgatgaga tccggccgcg cttcggtctg atgcgcgcca aggagttcat
    26221 catgaaggat gcgtacagtt tcgacacctc catggaggcg gcggaccgtt cttatcaggc
    26281 gatgtacgat gcgtataccc ggatattcca gcgttgcggc ctgcgggtca agccggtgga
    26341 ggcggatacg ggggatattg gcggaagctg gtcgcatgag ttcatggttc tggccgactc
    26401 gggggaggac ggcattgtgg attgcgccgc ctgcggatat gccgccaatc aggagcgggc
    26461 tgaaaggatg ggcaagcctg cggcggaagc ggcctgtccc cccttgtctg aggtgcccac
    26521 ccccggagtg cgcacgatcg aggacctgac cgcctttttt gactgtgcgc cggaccggtt
    26581 catcaaaacg ttgatctatc tggcggacgg caagcccgtc gccgcattgg tcccgggtga
    26641 ccgcgatgtg aatgagcata agttgaaacg gctgctcaac gccagcaaga tcgaactggc
    26701 ggatgaggaa acgatcttcc gtgtcaccgg tgcgccggtc ggattcgccg ggccggcggg
    26761 gctttcgatt ccggtgtatg cggatcagtc gctggagggt gcggaagacc gtgtcaccgg
    26821 tgcgaaccgc gccgacacgc attaccggca ggtttccctc gcacgggacg cgcgtgtgac
    26881 cgcatatggg gacctgacgg ttgtcggttc cggggatctc tgcccgcggt gcggcaaacc
    26941 cctgcaactc cggcggggta ttgaggtcgg ccacgtcttc aaactcggga ccaagtattc
    27001 cgagtcgttc gatgccgtct atctcaacga aaaaggccag agcgagcaga tggtcatggg
    27061 gtgttatgga atcggcgtga cccggacgct gcaggcggtc attgaacaga gccatgatgc
    27121 cgacggcatt atctggcctg tatccgtggc gccttttgcg gtaagtctgc tgttgctcga
    27181 tccccgtgac gagcgggttt acgatgtggt cgctcaactg gaggcggaac tggaggcttg
    27241 cggcattgat gttttcgtgg atgaccggga ggagcgcccg ggggttaaat tcaaggatgc
    27301 cgatctaatc ggttgcccgg tgcgcgtggt ggcgggcgcg aaaggcctcg cgaaaggcgg
    27361 ggtggaaatc aagttgcgca caggtaagga gagcgagatc gttccggtcg agcaggctgc
    27421 ggcggcgatc gccgaccgcg taaaggcgtt gacatccgca ctcagtgcga aagcggacga
    27481 gcctgccgcc tgcgaagcgg ggaatgggtg agggtagaat ggaacggtta cgtccagggg
    27541 gaaggggagg gatgccgtga agccgtcaaa gcatgataaa gcgccggaaa gggtggcgtt
    27601 gaaaaaacgc gatctcgtca accggatcgc caaagatacc ggactgacgc aactgcaggt
    27661 tctggatatc gtccagagaa cgctggatgg tatcgtcgac gcgctggaag ccggtcagca
    27721 tgttgagttc cgcgagttcg gcgtgttttc ggtggttaaa cggaaagcgc ggatcgggcg
    27781 taatccgaat caacccgccg acgtggtcac gattccggtc cggtatgccg tgaaattcaa
    27841 acccggcaaa cgcatgcgag acatgcttgc ggataaagtg tagtgggctg ccgcacgctt
    27901 ttaggtttcc cgtcatgaat gtacccgctt cgttttcctt tgatccgccg cgcggcatgc
    27961 gcgattttta tccggaagac atgttttggc gcaaccggat ctttgacgcc tggcgccagg
    28021 cggcgcttgc gttcgggttt cagccctatg acgcgtgtgt ggtggaaagc ctcgatctgc
    28081 tgcagcggaa aagcggggag gaggtctcgg agcagattta ccattttgag gacaagagcg
    28141 gccgaagact ggccttgcgt ccggagatga ctcccaccct tgcccgcctg attgccgcac
    28201 ggcaggggca actcgtgttc ccggtcaagt ggttcacggt ggcccaatgt ttccggtatg
    28261 aacggatgac ccgcggtcgg aaacgtgaac attaccagtg gaatctggat gtcgtcgggg
    28321 aaggtcaggt ctcggctgag gttgaggtgt tggccgcggc cgcagagggg ttgcgccgca
    28381 tgggactgcc ggagcaggct taccggattc atgtcagcaa ccgtgccctg ctcgcggagt
    28441 tgctggcagt atccgggatt gcgcctgaac accatgccgc agtcttcctc gcgctggata
    28501 agcgcggcaa aatttcggat gatgagatcg ccgggttgct tcgggccgaa gggttgaaag
    28561 accctgcggt ggaacagacc ttcgctttgc tcggcattca aacgctcgat caggcagcgg
    28621 agcgggtcgg tcccgattcg gccgcggtcg ccgcgttgcg tgaattgttc gcgctggccg
    28681 gggtctacgg catcgcggat cgtttgatgt tcgatattgc cgtgatccgc gggctcgcct
    28741 attacacggg gatcgtgttc gaggcgttcg attcggcgcg caaattccgt gcgatctttg
    28801 gcggcggacg gtatgacaat ctgctgacta ccatcggggg tgcgcccacg cccgccgtgg
    28861 gattgggttt cggggatgtg gtgatcgcgg aggtgctcaa ggacctactc agcgagacgg
    28921 cggcaacgga aaagaatggg gtgatcatcg gatacatgtt ccctgaacag cgtgaatccg
    28981 cgatgcgttt ggcccggagc ctacgcgagg cgggtgagag ggtggacctc cacctcgcac
    29041 gccagaaacc gaaaacgttt ttcgccaaag ccggtgcggg ctctgcggcg gacgcgattt
    29101 tcatcggtcc cgatgatgtg gcgcgcggca cagtccggcg caagcatctc gctacgcgcg
    29161 aggaaacagt cattccgctg ccggaggcgt gaacggcatg cgcccggcgg tgtccacggg
    29221 aaccgacccg accgggttgg gttgccggga cggaagcggt ttcgagacgc gccgcgcagg
    29281 tcacgcgaaa agcgagcgga caaaatcacc gttgcgtttg ccgtcggcca caccgtcctt
    29341 ggaggggctt gaggtctcca gtacgatcca tcctgaatac cggatctccc ggatcgcagc
    29401 cgtgaccgcc tcaaaatagg ggcggtcctc atccagatac cgcgggccgt ttttgtagtg
    29461 gatctgcgta atacgccctt ttagaagccg gatttcggcg ggcgagtcgt atttcatgct
    29521 ttttccggta ttgtacacat cataataaag gctgaccgcc tcatgcccga tcgtgtccag
    29581 cattcgaagg ttctgctcgg cgctcaacag gttttcgatg gagagtgtga ctccggcgtc
    29641 gtgcgcccgc ggcgccgcat cccggattcg tcggcaagcg gaggcgaatt ccggctcctt
    29701 gatttttcga tcggcttgga ggtcgccttt gccgaaaaac gccaccagga tattcggcac
    29761 gcccagatcc tgcgccgcct caatactctg ccggagccag gcgggggcac gaggatccgt
    29821 ggcgagggga tacgagttca acagtcccaa catgagggaa caaatcggca atccggtctc
    29881 gcgaatctgc gttttgagac gggcgcgggt atccgctttg tagatatcca gttcatccgc
    29941 cgccccgccc gcccagagtt gcacaccatc cagtccggcc tgtcgggctt gatcgagacg
    30001 gagcgtgcag gaaccgactc ggattttcgg tttatccgca tgcgcacccg gggacgggat
    30061 cgcggtgagc gacagggtgg cggcggaatg ccggaggaac gatctgcggg taaacttcat
    30121 gacgtctcct ttccttgcaa cgcacgttaa cggaggacaa accccgtctt cggtgagaac
    30181 gtgatccggt aacggcaggt gggaacctcg cggctttcga cgcggatcag agccagcggg
    30241 ttgcggaatc cgatctgcag caactcctcc agcgtgaaca ggttgagccg gtggagcacg
    30301 ttcatacatg cgatcagcgt ggcggaagaa ccgaccaggc attgtttctc cggattatgc
    30361 aaccgtccgt ccggctccag tacggcctga ttccccagca cattgtaggt cccgggcggc
    30421 aatcccgctg caggcgaggc gtccgtagtg gcgatcagac ggcgcgcgcc cgcggcacgg
    30481 gcaaacactt tcagcacgtg ggcggggaga tgatgcccgt ccggaatgaa caggatctgc
    30541 aagcgctcct cggccaagcc ggtcaacagc ggattgtcgt gccgatgagc ctgattcggg
    30601 cagccgttcc ccagatgggt cagggccgag gcccccgcat caacgagccg cgcaagggct
    30661 gcggcatccg cgagttggtg accgcaggag acaaggatgc cccgcgccac ggcatgtcgc
    30721 gtgaggactt cggcaccggg aatctccgcc gcgatcgtca gcaggcggat ccggtctccg
    30781 gcccactggc agagccggtc gaaatacgcg caatccgggg gacggaccgc tgcggggtca
    30841 tgtgccccca ccgctcccgg ctcgctcgaa atgaaggggc cttcgaggtg gaatccggga
    30901 atgtgacgcg ccagcaccgc atcttttttt aaaacggacg ccatccgctc aagattgcgg
    30961 cgggtctgct ccggggtgcc agtgattacg gtagggagaa aagcagcggt gccgcgttcc
    31021 agtagcgtat ggcaggcgag tttaaagctc tcttccgtga gatcgggacc gctgaaatcc
    31081 acacccacaa acccgttgac ctgaagatcc acaaatccgg ggatatccag tttgacatgc
    31141 atgctgagag aataagggat tcgatggggc ggggcaagga tggaataccg gaatccgtct
    31201 ggcgttttcc ggggcaggtg tgaggttgcg cgggactgcg cagatgtgtt atagtttcgg
    31261 aaatcttcga attatgagaa gggcacggca tggattggcg agcggagttg agcgggattt
    31321 tgggcgggag agcccggacc acgcgtgcgg aagaggaaaa cgctgttttt gaggcgtttt
    31381 tggatacggt ggccattccg gcgttgtccg agatcgcgga agagttggtt cagaagcacg
    31441 aacgtgatgc gcaagtacgg cgggcgcccg cttcggcgac gttgagtgtg cgcatcggtg
    31501 agatggagga aatcacattc cgggtgatga agcagttcgt ccagaacggg attctcccgc
    31561 gggcggaagt gcgcttgaat cgcggacagc ggctggtcaa atacgagagt atgttcagag
    31621 cggatcctca aacgtatccg atcacggaga taactcagga tgaagtgatc gcctgcttca
    31681 taaaatatta ccgcatggtg atggatcgaa acccaaccgc agccgagtga gtgccgtaaa
    31741 gaccggcgtg tgcgacgccg tgtttcggaa aagttgatgt cgtgaaggat tccgtgcgta
    31801 tgatgaaaat cccgttgccg aaaataatcc agggtggtat gggtgtcggc gtttcccatt
    31861 ggcgtttggc ccaggccgta tccaaactcg ggcagcttgg tgtggtttcc ggaaccgggc
    31921 tcgatcaggt tctcgcccgg cgtttgcagg atggggatct ggggggtgac gtgcgccggg
    31981 cgctttcgca tttccctttc ccccagatgg cgcagcgggt gttggacgca ttgtttgtgc
    32041 ccggcgggag gccggcggac cagccgtatc aggctgcgac caaacatacg atcaaaagca
    32101 accggtgggt ggacgagttg tgcgtcgttt ccaattttgt ggaggtcttt ctcgcgcgtg
    32161 aaggccactc caatccggtc ggcatcaact atctcgaaaa aatccagttt ccccacctgc
    32221 cctccgtcta cggcgcaatg ctcgccggtg cggcggtcat gatcgtcggc gccggcatcc
    32281 ccgtggagtt tccgggcgtg ttggatgcgc tgtcgcgcca tgaagccgcg acctataccg
    32341 tccgcgtcca cggcgcaacg cctgagaccg actgccagcg tgtctttgat ccggccctct
    32401 tttttgagga tggctgtacg ccgccccgtc tgctgcgtcc ggatttcctg ccgatcatct
    32461 cttcggactc gcttgccacc atgttgttgc ggcgcgccag cggtagcgta gagggttttg
    32521 ttgttgaggg accgacggcc ggcggccaca acgcgccgcc acgcggtccg ctacagctga
    32581 cccccgacgg ccagccgatc tacggtccgc gcgatgtggt caaattggaa tccatgcgta
    32641 aaatcggcat gccgttctgg ctgggcggtg gctacggatc gcccgaagcc ctgcgcacgg
    32701 cactcgcaga gggtgccgtc ggtgtgcagg tcggcacgcc tttcgcgctc tgcgaagagt
    32761 cggggctgga gccgaaaatg cgccacgctc tgatccatct ggcgctggct ggcgacggca
    32821 aggtctttac cgacccgctc gcctcgccaa ccggcttccc cttcaaggtc gcacggctcg
    32881 aagggacgct ttccgagccc gaggtttata cggcgcgccg ccgggtgtgc gatctcggtt
    32941 atctgcgcga ggcctatgtc caagcggatg gcgtcatcgg gtaccgctgt cccgcggaac
    33001 cggttgaggc ctacacggcc aagggcggca gtgcagagga tactgtgggc cggaagtgca
    33061 tctgcaacgg cctgttcggt aatatcgagc tgggacaggt tcgggcggac ggcacgcgtg
    33121 agccggggat tgtcacgctg ggtgacgatt atctgcggat cggccgtttc tgcacgcttg
    33181 aacagccgga ctattcggcg tcccacgtcg tgcgtgttct gctgggttga ggcggcgagt
    33241 ctgccatacc gcgctgcgct ggaaaacgca tcggtccgaa tcagagcgta aagcccgcaa
    33301 gcagcgtttg ccgccagtcg cgcatgagcg ggtgcgcgga ggcgtctccc cgcgcaaggg
    33361 actggaccag gctttccgct tcgttgcgcg aggcgatcca tgccggatcg atcccctctt
    33421 tttcgcaggc gatccggaga tgggcgagca ggcggtcggt tggctctttg agtgcgcgcg
    33481 cggtcgcggg attccggtca gaggggtcag ggcatgcgct ctccggcaac gcctttcccc
    33541 gttccacggc cgccaggatt tgtccgacaa gtttggcggg catacgtgaa ggaaaaccgg
    33601 ggaccgccat gagcgcccgg acatctgccg gacctcgcaa cgcgatcgtc accatcacgg
    33661 ggtcgggcag cacgtgattc cggggccagt cacggcggcg ggcttcccgt tcgcgccacc
    33721 ccgccacctc gcgcaggagt gcgaggtggc gccggttcag gcgggcggcg cctttcacgc
    33781 gccgaaacct ttcctcaggc ggagactcct cgtaatgtgc cggatcgtcc agccgcgcga
    33841 gttcttggtc cagccagttc cgggaccgct ttccggcgca gcgcgccagc agggcttcgc
    33901 gaagcggaag caggtgaaca acatcctcgg cggcatatcg gatctggttc tcgctgagcg
    33961 gtcgccgtaa ccaattggaa cgggtctcgc ctttggcgat cgtcaccccg aggacctcct
    34021 gcaacagggc ttggagcgag agcgttgcgc tgagtccggc gaacccggcc gcaaggcgcg
    34081 tgtcgaatac ggagcgcggt acggcaccgg tcgcgcgcgc taggatcgtg aggtccagag
    34141 gtgcgtcatg cagcactttg gtcaccgacg gcgaggcgat caacggggcg agcgcagaga
    34201 gatccttaag gcataccgca tccaccagcc agcacccatc gggcgtggcg agttggacga
    34261 gtccgagaac cggatagaaa gtgcgttccc agaagaattc cgtgtccagc gcgacggctt
    34321 cggcgcgcag cagacgggta ttcaggtcgg cgaggtctgc ggccgtagtg atcagggttg
    34381 gtgtcaacgg caccccctta ccgctttggc aggtggatgc aggcgtcatg aaatgcgtgc
    34441 gccgcctcca tccacgcctc cgagagggtc ggatggcagt ggatcgtatt gccgatctca
    34501 tggaccgtca gttcgttacg gatcgccagc gcggcctcgg cgatcagttc ggtggcgtgg
    34561 catccgaccg cctgcgcgcc gagcacctgg ccggtgaccg gatcgcagac ccacttgacg
    34621 aagccgtcgg tctctcctgc ggtcatggct ttgccgagcg ccgagaaggg gaaaaccgca
    34681 gtcttgaccg caatcccgcg ctgccgggcc atgttttcgg tgaggccggc gagaccgatc
    34741 tcgggcgagg tgaagataca ggccggacag atccgctcaa ccttggattt caggcccgcg
    34801 gcggcttcgg cggctgtcac accctgctgg gtcgccgcgt gtgcgagttg gatcgaaccg
    34861 gttaccagat caccaaccgc aaaaacagat gcaacattcg tgcgcccctg cgcattcacg
    34921 gggattgtgc cgcgctcatc ggtcttaatg cccgcgcggg caaggtcgag gagggcggtg
    34981 ttgggcttgc gtcccacggc tacgagcagc acgtcaccct cgacgcgctg atccttgaag
    35041 gttccggtga ccttgttttt tttcacggcg atgtcggtca acggcgtacc ggtgaggagt
    35101 gtgatgccga gcgtttccat acgtttgcgc aacacgcggc gcacgtcgcg gtcgagcacg
    35161 gtgacgatgt cctcgagcat ttcgacgacc gtgacgtccg cgccgagcgc ggcggccata
    35221 caggcgaatt cgcagccgat cacgccgccg ccgaggatga gcagcttctt ggggagttcg
    35281 gtgagatcga ggaaggcacg gctgtccatc acgcgcttgt ccttgggcag gaaagcgggc
    35341 atggccgaat cgctgcccgc cgcgatgatg atcgagacgg cgctgagcca gcgcgtggtg
    35401 ccgtcgggct ggaggacgga gaggcggttc gggccttcga agcgggctgt gccgttgatc
    35461 agctcaacgc cattcgattg gagcagcatc cgcacgccgc cgctgagctt gtcgacgatt
    35521 tcggccttgc gggccgtcat cgcggcatag tcaaaggtca cgcctttggc agtgacgcca
    35581 aacgttgcgg attgggtggc atgatggtaa cgctccgcag aggcgatcag ggttttggtg
    35641 gggatgcagc cccagttgag gcaggttccg cccaacgcct ctttctcaat gatggcggtt
    35701 tttttgccga gttgtgcggc acgaatggcg gcaggataac cgcccggtcc ggcaccgatt
    35761 acgatgatgt caaagtcttg catgagaacc tcggggattg gaatggtgga gtgaagggaa
    35821 gagggaccgg tattctctat ttatacgagc gatttccaca gttcgatgtt ttcgagtttc
    35881 gaacggaccg cgttagcgaa ctgcgcgccg acggcaccgt ccacgatccg gtggtcgacc
    35941 gagagggtca tggccatccg ggagcggatg cgcacttcgc cgttgacgac cgcaggctct
    36001 tggcgtccgc tggagaccgc gagaatgccc gcctcgccgg ggttgatgat ggcggtgaac
    36061 tggtccacat tcagcatgcc catgttcgag acggagaagg aacttcccgt catctcatcc
    36121 ggcgtgagcc ggcccgcacg ggccttttcg gccagcgctt tggcttgtgc gctgagttca
    36181 ctgaacgtga gggtctgggc ggcgcggata accggcacga ccagaccgtt gtccacgctg
    36241 accgccatac cgatatccac atcgccgcgc cacgaaacgg tctggccgtc cgtgacggag
    36301 ttgacgatcg gaatctcctc aagcgacaga accacggcct cgagcacgaa atcattgacg
    36361 ctatagttca ggccgcgggc cttgagatca gaacggaaaa ccatcaggtc ggtgatgtcc
    36421 gctttgactg tgacataaaa gtgcgggatg gtctgcttac tctcgaccaa acggcgcgcg
    36481 atgacctgac gcatgcggga gagggccacg ggtttgcgcc ggatcgcacg ttcgatgtcc
    36541 tccaccataa tccgtccgct gtcacccgaa ccgcgaaccg tcagaatgtc gatgcttttt
    36601 tctgcggcca gccgtttggc cgccggggtg atacgcagcg cgtcgtaacc ggatgcggcg
    36661 agataggccc gcacgtcctt ttccacaata cggccgttgg gaccggtgcc ggtgatgcgt
    36721 gccgggttga tacaggcgct ctgggcaagg gttttcgcgc gcggcgaaat aacgagacgc
    36781 gctgcaggga cagggacctt cgcgggctgc ggtgccggat gggagggagg ggcaggcacg
    36841 ggagcgcggg caggcacggg cgccggagcc gccgcaggct ccgccacggg aacgcgggct
    36901 gcgccagggg tcggcgaagg agatgcgaca ggcttgggcg cgggtgccgg ggcttcgggc
    36961 ttcggtgcct gatctggaac cttttcgccg ggagcgccga tgtagccgac aacggtgttg
    37021 accgggaccg tgaccccctc gcggacatag atcttcagca ggacgccctc aaagaaactt
    37081 tccacctcaa gattcgcctt gtcggtttcg atctcgaaaa ggatgtcgcc tttgacgacc
    37141 gggtcacctt cttgtttcag ccattggacg atggcgccct cttccatcgt ctgaccgagt
    37201 ttcggaaaaa tcactgcctg ggccatagcg tctccatttt ttgaaaagta aaaatatcat
    37261 tttagcattg cctgaagcga ttgtcatttg caaaatcaat caaaatcaaa aaaaatcgat
    37321 caaaaccgag cgcggccagt ttatgctttc tctgcggccg ggaccgggat atcctatctg
    37381 ggttgccggt tggcgtcgga atccgtttcg gtttagtctg cggacctgtc gaggaggcgg
    37441 atgattacgg ttttacatgt gctggaggcg acggagggtg ggacgcggcg tcacctacgc
    37501 gatctggtcg gcgcgctaga gccgttgggg ttccgttgcg ccttggcggt ttcgtgccgc
    37561 cgcgatccgg cctttcgcag ggatgtggcc ttctacctgt cgcgcgggct gacggtggcg
    37621 gaacttccca tgcggcgagg tctggcgccg attaccgatg tggccgcgtt gttgcgcctg
    37681 atcgggtgtg tccgccgtgt gcggccgtcc gtgatccatg cgcacagcgc caaaggcggt
    37741 ctgctggcgc gtgcggcagg tgcggtctgc cgggtgccgg tggtgtatac gccgcacgtt
    37801 ttcccatttc tcatggcgtg tgatccgcgt atgcgccgcg tataccgact cgtcgaacgc
    37861 ggactgcggt gcatgaccgc cgcagtgatc gcggtttcgg aggaagaacg gcgcgaggcc
    37921 gcggcgctgg ggtatgcggc gtctcgaatc tatctgatcc cgaacggagt ggacggcaag
    37981 gttcccggcg aggtcacggt gcgtaaggat ggggagttgc gggtggggtt cttcggacgg
    38041 ctgaccgccc agaaggggcc gtccgtgctc ttggaggcgg ttgcggatgt ggtggcgcac
    38101 ctgccacagg tgcggttcgt gttctacggg gacgggacgg aggcggacac gttgcgtgcc
    38161 cgggcggaag tgctgaaact gaacgatgcg gttcgctttc tgggatctgt cgcgcaggga
    38221 gaggtggtgg cgcacatgcg gcagacggat gtgatcggga tcccgtcgcg ctgggaaggg
    38281 tgtccgtatg tggttttgga ggctttccaa gccggggtgc cggtcgtggc cgcggcagtg
    38341 ggcggagtgc ccgatctgat ccgggacggg gtcaccggtg tgctggttga gccggacaat
    38401 cccgaggcgt tgtgcgatgc gctgctgtcc ctgttgcgcg atccgcagaa acggcgtcgg
    38461 atcgcggaac aggcgcggaa tgaactggca cgataccggt tggatgcgat ggcggctgcg
    38521 gtggcggacg tttaccgccg cgtcacgcaa gatctgccct gatgcgtttt caggccgaac
    38581 ggtttggggt tgccagtgac tttttcactt ttgcagggtg ctgcaccagc ttgcgaacct
    38641 cttccggctg cattttcgct acgacggaat cgtcaacgcc gatcttcacc aaacgggcgc
    38701 gctgcgcctt caggcggcgg cgcttctcgg cctgactttt gatgggacgt gtgcgaggag
    38761 ccttgtttaa tgtacggcct gtgcccatga aaaaaacctc caaccgattg ctttcagtaa
    38821 tgagataagg gcaacatcat ggcggattcg ggaggaagat gcaagccctc gttttaaacg
    38881 tgccgcgcaa cataaaacag cctcacttgt caaaggaaat gccctgccgc aatctttcgg
    38941 ggtggaatcg gcaagccgta taagctattc taatgcgctt tcaaggaggg tacgcatgtc
    39001 tgtttcggtc gggttcatca gtctcggctg ctctaagaat ctcgtggatt cgcaggtgat
    39061 ggccggatat ctgaaggccg gggcaatagc gttggcacct tctcccgaag aggcggacgt
    39121 ggtgttgatc aacacctgcg cctttatcga gagcgcgcgg gaggaggcgg cgggggcgat
    39181 tttgcgagcc tgcgcgcaca aggagcgggg cggttgccgg gcggtgatcg tggcggggtg
    39241 catggtgcag cgttaccggt cgcgattgaa agccgcgttt cccggggtgg acgcgtttct
    39301 cggcattgat gagttggagc ggatcggcga ggtggtcggg aacgtggcca agggtaaacg
    39361 gtgcggggtg atcgcggcgc gcggcatgcc ggtcaaatgc tacaaaccgc cctatccgac
    39421 gctgctcttc acgggtggcc ccttcgcgta tctcaaaatc gcggaggggt gcgatcatcg
    39481 gtgcgcgtat tgcgcgatcc ccaacatccg gggggcgtac cgttcgcgct ccgaggatga
    39541 gttggtgcat gaggcgcagg cgatggtgcg ggcgggtgtg cgggaaatca acctgatttc
    39601 ccaggacaca ttgcggtatg gaacggatcg cggggggaag tcggcaattg tgccgctgat
    39661 ccgccgactg gaccggatcc ggggtgcgtt caggattcgt ctgctctacg gttatcccgc
    39721 cggtgtgacg aaggaactgc tgcatgtgat gaacacgtcg caccatgtct gctggtattt
    39781 ggatttgccg atccagcaca gtcatccgga gatcctgcgc gcgatgcgcc gcggtaaggc
    39841 ggtggcggcg acgcaggatc tggcggcccg actgcgccag acggtgccgg gactggtgtt
    39901 gcgcactacc tgtctggtcg ggttcccggg ggagacggag acgcacttcg agcacctgat
    39961 ggagtatgtg gagcggtcgg ccttcgacca tttgggcgtg ttcgcattct cacccgagga
    40021 gggtacgcct gcggccgcga tgggcggaac gccgtcccgc aaggtggcgg aagagcggtg
    40081 tgcgcggctc atgaggctgc agcggcgtat cgtccggaga cgggtgcggg atttgacggg
    40141 gagggaagac acggtcttgt tgctccggcg cacaaaaacc ggatgggtcg ggcgtctgcc
    40201 gcggcaggcg ccggacgtgg acggtgaaac ccatgtgtcc catgtgccgc ggcaggccaa
    40261 ggttggcgat ttcgtgcgtg tggaaatcac aggtggcaga ggttatgatt tgatggcgag
    40321 ggcggtttga tggatacgac ttcatttttt caggatttgg ctgtcatttt ggtggcggtc
    40381 gggattgttt cggccgtgtt catgcgtttt gggtggccta aggtcatcgg gtatatcctc
    40441 gccggggtgg tcatcggcaa gcataccttg ggcgggtcgc tcgtcatcaa ccagagcagt
    40501 atcgacgtgt tcgggcagat cggcgtcgtt tttctgatgt tcacgctggg gctggaattc
    40561 agtctgcgga agttgaaaaa gatcggccat gtcattttcc cgacagccct gttggacatg
    40621 acggtgatga tctgggccgg ccattttgtg ggcacaaaga ttttcgggtg ggggtcggtg
    40681 cagagcctgt ttcttggggc ggccatcagc gattcggcca ccacgttgct ggcgaaaacc
    40741 atcaacgaca tggggtgggg gacgcggcgt ttcaccaagt atattttcgg gatcaccatc
    40801 accgaggata tcctgtgtgt gggtgtgatc gcgctgctta cgggacttgt gaggtctggg
    40861 acgatgcgtg tgggcgcggt ggcgtacagc atgggggggc tcctgctgtt tctgaccggg
    40921 gtgacggtgt tcgggctttt gttggtgccg cgcgtgttga atcgcgtggc acggttgaag
    40981 gatgacgagt cgttgctgct gaccatgctc ggcttctgtt ttctggtctc cttcattgcc
    41041 gccaaactga atttcagtct ggcgatgggc gcgtttcttg tgggggtgat gggggcagag
    41101 tcaaagccgt tgaagcggat ttaccagcag tgcatgccct tgaggacgat gttcagcgcg
    41161 attttctttg tgacgatcgg gttgctggtc gatccggcgc aaatgctggc caacgggccg
    41221 gccattttgg gggtcacgct gctggtgatt tgcggcaaga gcttcaactg cacattgggg
    41281 gctttgctga cggggcagga tcttaagaac gcgattcaga ccggtatcgg gctggcgcag
    41341 atcggcgagt tcgcctatct ggtggcgctg attggtctga ccatgaaagc ggtggacagc
    41401 tccgtgtacc agattgcggt cggcgtgtcg gtgctgacga cggtgctgaa tcccttcctg
    41461 ttgcgggcat cggatccgct ttcggattgg gtggtgcgca aattgccgcc gcggtggaaa
    41521 gagtggttgg agacctattc aaactgggtg gaccggttcc agcacagctc ggcacccagt
    41581 gctacgatga aggccatccg ttttaacctg acactcgtgt tgctgcatct ggctttggtc
    41641 gcggtggtct atatctcggc gggtatgttg gcgcgggtcg attacacccg gttctcgccg
    41701 ctgatcgagg agaacaagaa aatgttgctg tggagcgcgg cc
//
LOCUS       DAOSLE010000009        39321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1190_length_39321_cov_8.097463, whole genome shotgun sequence.
ACCESSION   DAOSLE010000009 DAOSLE010000000
VERSION     DAOSLE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 39321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39321
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1190_length_39321_cov_8.097463"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK576_01390"
     CDS             complement(<1..496)
                     /locus_tag="PK576_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE60275.1"
                     /translation="MAAKKIDKILVINSGSSSLKFTLFNVSTKTALAKGLVERVGTPN
                     ANLVYQRNDEPKITQGIAAENHGKALAMACKMLTDPETGVIKSLKEVNAIGHRVVHGA
                     EEFSDSVLITEDVKTSIKRCADLAPLHNPANLDGVVACETVFPNTPNVAVFDTAFHQS
                     MPRHA"
     gene            752..2098
                     /locus_tag="PK576_01395"
     CDS             752..2098
                     /locus_tag="PK576_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60276.1"
                     /translation="MGTNRRRFLKQAAVAGMPFILPSHIWAAPVKPNDRMTLAFIGTG
                     NQFSTLVSPFLYEPVQALVACDCDTFLRESAQQRINEFYKGKPEKGSPCCEAIADFQE
                     VIARKDIDLICVATPNHWHAYITIAALKAGKDVYCEKPLTHTVQEAVQVMKTVRETGR
                     ILQTGSMQRSMNEFRVACELVRNGCIGKISHVDVHFDEPGRPYDMKEEPVEPGLDWNR
                     WVGPARMVPFNRAVIFRPAPLKPYARRWREFKEFCGGYIANWGSHHIDIAQWGLDMDA
                     SGPVTYIPPEKPDAQWGAEMVYANGVRMFHKPGFSQGVTFYGSDGKISVYRGIFHFER
                     DGKVISHFWKKGDPSSCGAEVAKAEREYLKDAKVKLYRSRSHVENFIKSVKTRQPTIS
                     NAEVGGHTAICCNLLSEAYYHRQRLQWDPVALKLVEGDNKWTAREYRDWGGPRSPA"
     gene            complement(2133..2825)
                     /locus_tag="PK576_01400"
     CDS             complement(2133..2825)
                     /locus_tag="PK576_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOE60277.1"
                     /translation="MDVLIYTRNYTPDGKGYVHDNIATSVAALRELCAERGLKTEASD
                     DPTLFTEARLKGLKAIIFANTNNEAFTTDDQRGALLDYVYCGGAFVGLHSATGSERKS
                     EAFRRMLGGRFLWHPPLQPFTIRVSDRRHPATAHLDATWSWTDEPYVCDCTPDIHVLF
                     EVDVASLQKKPRDKRLDTFKSDSFPLAWCRRHGAGQVFYTALGHLKTHYQDPAFRTHL
                     IQGVLWAMSANA"
     gene            3274..3390
                     /locus_tag="PK576_01405"
                     /pseudo
     CDS             3274..3390
                     /locus_tag="PK576_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586894.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(3625..4287)
                     /locus_tag="PK576_01410"
     CDS             complement(3625..4287)
                     /locus_tag="PK576_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60278.1"
                     /translation="MTRVIKALSRNKLVGREKQVFAECNRKLEEARKHRAQARQSATG
                     ATPCTCRFKMLTRPADQDRVDNAIRAVAVGPVTKSGTVSYPEYRFRVARLSDLDRLHP
                     QLLFDNPDALLTANRRQVLNMRAAGVDVTFDSTDVSASATITVTFNVKPGSRLYYKQP
                     GGVETDITAKVDKRGKVTFPASIKEGQKFIYARAVKDNVTRYIRINIFSNEVQDIRRN
                     EY"
     gene            complement(4284..6299)
                     /locus_tag="PK576_01415"
     CDS             complement(4284..6299)
                     /locus_tag="PK576_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60279.1"
                     /translation="MKKNLGMKKSGWQMFMASLVGAWCALAGEPYYQTAFETAEEIRG
                     WKRDANWKIEEGAGTGGSRALVWTNDDPKNYNVNSFTIPGGRPGLYFVATFKVKPEKI
                     TGARLGCTICWTGKDKWLGGAGGTVMKLGDKRLKPDADGWYEMTIRMPSERLPEEATA
                     VQLQLYVHAGGTGRVAFDDIRVDTFADPKKAAVKTLTTSCYRDMAATGIVHVRAGLEL
                     PDKYLGEKGLVTELAYRASDGARRQDAMRAVEAKQFVASIPVTSFAMGQHPLVATLKL
                     ENGEVLGEKTLVFERVDTLPPRRVCFDRYGRTLVEGKRFFPLGMYWSENTLAKSNSLE
                     RYASGPFNCLQTYEKQMTPEILDRYWRHGLRVVASVKDIYVRPVGGPLIGFTPPEVKT
                     RTDETNYVKRVVERCRNHPALLAWYTCDEIPHEYADRVEDRYSLLKQLDPDHPTFICL
                     NHPVTARVFIDCTDVVGSDPYPIPGSDRVNTVLPPDAGAVWRAGDSARTIRESVFGLR
                     PMWQVPQAFAWKWDKSEQPELRFPTRRELSSMTWQQIAEGANGLLFYSYGQMMNKAGA
                     ELEENFRTTCSVAVEVRDLIPILLLEPGPDVRKMPPRTRVRTWKDGKKVYVLVCNTHP
                     DRRVGQVEIEGAYTTCAPVFGSGVTRAGGVLSLDMDSMGTAVVRLEP"
     gene            6642..8525
                     /locus_tag="PK576_01420"
     CDS             6642..8525
                     /locus_tag="PK576_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60280.1"
                     /translation="MTRTLRLITICSLLAACAGLLSAAPEAEAYRIRRGIPNSRYFLQ
                     NDTVGNQYLFFIGHSVLAGTGLKNSGLRYSACMTKALKRHFPNASPRETRHMYPGGSW
                     FAQYRCSRGQPVFGEVICSGHLAILDFAAEDRSADLEQVKTSLEGLIRQITLYRATHS
                     TILVYTLTPEMLADYRAGRTPAYIEVCERIADHYGIPSLNLAQVAARAILSGTISAEA
                     FSADGIHPTDAGARLWAEAINAFVDALMADGPVPEKPQRVTLPAPLFPGTDDRGQIIA
                     YEHPEVRTTGSWQPGRESPIKPFRHVLVSREPGTRLTLRFQGSEIGLIDVAAPDGADL
                     EYAVDGGAFRTLAAPKNTDKPVLRALPLARGLTRDAEHEVVIRTASDGTARIGGLLVN
                     GSVKNPHAGMSTLERIDAIYAAMDPINYTPPANRFAHIPKTMAKLRGGGELRMVLLGD
                     SIMGNTSGSSFELLLMRNAPACKIIKIASLRSSTGCTYYQHENRVQQYVLRHNPDLLM
                     IGGISNGGDAEAVRSVIRQVRASKPDTEVLLLTPVFGAMRDEHIRTFTREIDTTTPNF
                     RHRLSKVAEEERCAFFDMTGPWWEYIQKSGKTYGWFMGDAVHANARGCQIIGRLLERW
                     FKE"
     gene            complement(8515..9621)
                     /locus_tag="PK576_01425"
     CDS             complement(8515..9621)
                     /locus_tag="PK576_01425"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOE60281.1"
                     /translation="MPSLPIFSLSDRSVPPLILAPMAGFTGAPMRALCRAHGAELTYT
                     EMTSAAGLLHSSDKTWHLLETFADEGPVVAHLYGSEPEILAEAAARVEATGRFAAIDL
                     NAGCPVRKIAATGAGATLAADPQRVHAILKAMRRAVTLPLTVKTRLGPDPGRVTVFEL
                     LAAAEEAEADAFALHARFTSQGHGGEARLDLLAEVKRRARIPVIGNGGVGSPYTAQRM
                     LQETGVDAVMVARAAIGNPWLFSDIRDALVSGRTPSPVETARGRPRRALETIRSVLEA
                     HLEAECELARRCCEREGRAGSVRDLDAAGVAAFRGHLFRYLHGLKGSSYLRGRLHTLH
                     TLEEVRAAVEACLAREAAYRAGARPSGNSNRVTP"
     gene            9773..10555
                     /locus_tag="PK576_01430"
     CDS             9773..10555
                     /locus_tag="PK576_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE60282.1"
                     /translation="MREVLDVLLLSVLQGIAEFLPISSSGHLVLAQSLLGVNTPGMRL
                     EVVLHLGTLVSILVYYRNRLTQLVLGTVRGDRESWRTAGQIALSAMPAVFFYLLCHDK
                     INAFFESPRAVGGFLIFTGVVLYALRWMACGQGRLTAARALLVGLAQALAVLPGVSRS
                     GMTIAAGRMAGIAPDRAAEFSFLMCIPLLIGAALMDGLGITPAAPKADALPVWLLLAG
                     AAVSAGVGYLALSLLVRVLRAGRFWLFGLYCIAAGFVTILLT"
     gene            complement(10568..11443)
                     /locus_tag="PK576_01435"
     CDS             complement(10568..11443)
                     /locus_tag="PK576_01435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE60283.1"
                     /translation="MSPGLADEYELLDSGDGRKLERFGAYTLARPCSQAVWKPRLSEA
                     EWTAAAHAAFDREDGNRWHNRSGLPAAWEVEIAGIRFRLSGTDFGHLGVFPEQRAQWL
                     WLRNTVRDACAARPGPVRILNLFAYSGGSTLAAALGGAEVCHLDASKGMVTWARDNAR
                     LNRLEAHPIRWITDDAHTFLRREVRRERRYDAVILDPPTFGRGQGNETYKIERDLPET
                     LRLCRSLLAPDPLFVLLSAHTPGYSPLVLENVLGQAVGDLDGKIVSGEMLLTGAATVP
                     STPSGAFARWTRGAQ"
     gene            complement(11472..12620)
                     /locus_tag="PK576_01440"
     CDS             complement(11472..12620)
                     /locus_tag="PK576_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60284.1"
                     /translation="MVVAKFCLVGFVFAAPEIRDDAVRLDFFFSPGCEECERVKREAL
                     PELADQLDGLYELASHDLTDAESVPLLVAYQQRCGNTDNGRVALVIDHSVFLSGADLI
                     VTGLLDRVGEALVRRQQPGWQMPQAPILDDERAAEIVKERAGALTFSVVALGGLLDGF
                     NPCAISTLIFFMSVLALARADRRTRLLVGASFITASFFVYMGLGMGFLFAFRQVPQFV
                     MVKRIIEVVLGLAMVPLAILSFRDAIRFRKSQRPDDVTLQIPKKIKGQIHAFMNSRLG
                     VGGPVLGGLVTGAGVTLLESVCTGQSYVPVLMYMLKRDCSDFTSWILLITYNLLFVLP
                     LTVVFICFHRGLQLTSLINWSKRNLVVVKILLGFFFAAMALLLLWGKT"
     gene            12774..12953
                     /locus_tag="PK576_01445"
     CDS             12774..12953
                     /locus_tag="PK576_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60285.1"
                     /translation="MANVHVLQIAIGIGIGIAIAIGFEIDCCAHAVARSDKCNDSGDS
                     DSDTDGDSDGAAMEP"
     gene            complement(13116..15254)
                     /locus_tag="PK576_01450"
     CDS             complement(13116..15254)
                     /locus_tag="PK576_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOE60286.1"
                     /translation="MERITRRKFLKTVGAAAPAAGWVTGGAASPDAGGTEAFREAVTR
                     YLETLARSDGGYGWEDQEDSFLTETYAAVGAYHALGMRPPRPAETAAYVRRAHPINGP
                     YAQTRRHWAEIKEFVFQQIQTLLWLGEPAGEGFSRQVAGWRKVSGYTTAYEKGGNPVF
                     RQEVQPLLCRVLLGLPCETLAEAFGGYLEARRRPDGTFNNTPAADGSGGHLVNTFFGI
                     RAQQALGIAPRPAALDWIRRCQRPDGGFSWCPEPEVGGLSDLSYTWAAIRTLELLGGK
                     PVRPEACRDWIRSLWNADGGFADRPGGVSSALATYQACDILARTGGGLRDTGPQDRPS
                     RRRSVIPADLHAFTIQFEAPGEGSVIEAVELAKALKIHLWGAKNSRPGWREAAQACAE
                     ERGVPVTFFASDEAYGTRVSLPGLGSYTHVNDPVAAPGRPVPAWPRRDGSWREFRDEC
                     VGSLHAVGGMMLWQICDNEEFARIVLDDSILRGGYGAISTFHFGCHNMIWALPFMMRY
                     RHEIPFVSLQDAHGESWWWSGMLTGFRTLFLAREPGWEGWQEALREKRVVAVRRDART
                     AGRIRMLGGAPEVREACMRRLDDWRWWDDSGTVLDRMPVSVVTVRPGDRFEFGRPDAG
                     VCVRIRVRREWVEGRKLADPSAITCVGATLDGVAIKPERVAKRDAQGLLEECCELIRL
                     PASPAKRILNMNFREQTADGVREFTWRLEV"
     gene            15489..16625
                     /locus_tag="PK576_01455"
     CDS             15489..16625
                     /locus_tag="PK576_01455"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019977798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOE60287.1"
                     /translation="MINLKQALLTLSGAMCVFALPVRAEKILVFFGTGGAHAQGIYRA
                     SFDTRTGRLEPAALAAKIGSPGFLALHPDGDKLYAAANGEGGQGVTGFRIGGDGVLNA
                     FTAAPTGDGQACHLAVHPSRRLLLTAQYGTGSVALFPLDTEGRLGAATTHKHPGPGTG
                     VVAQRQKTPHPHWCGFSPDGRFALVPDLGLDGIMVYQIDATAPNIARHGFITAPRGAG
                     PRHMRFSTDGRRIHLLNELSLTVSTYAWDAETGSARLLTTVPTLSETEKAGEAHNSAA
                     EILTHPNGRFLYASNRGNDTVTVFRADPATTAPERIQVQPVRGAFPRNINLSPCGNWL
                     LAAGADSHTIAVHKVDPETGRLTYQTRSVISVPAPICILFARVP"
     gene            16947..18122
                     /locus_tag="PK576_01460"
     CDS             16947..18122
                     /locus_tag="PK576_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE60288.1"
                     /translation="MCPTRNPSDPKAAIAFTERQQGIVAAGITVLCATLITGFAVLLF
                     WALFALLSAVSVVLTPLLVAMILALLFKPYYQWLHAHLRRSHLLALPAFFLSLLIPVG
                     GLFFFFGSLLVTQLMALYDYLPSLIEQIRDALTHGNPALEAFITKFGLETKIPLLSDP
                     ESFVAALFDRVSLDSMGGMALAYGMDAIKHLVSLIGWLVVPVYLIYFLTAKPFSGKNA
                     ENFLPFLKPATRRDVSYLIDEFFSIIVAFFRGQVTVAFIQGLLFGFGFWIVGLPYGLL
                     IGLTLGLFNLVPYLGNIIGLAVTLPLAFFGEGGSGLRLGLVLAVFCIVQILDGYFITP
                     RIQGKKTGLSDPAVIFSLLFWGVVFKGILGVLLAIPLSAFIVVLWRLLKTKYIRELI"
     gene            18503..19471
                     /locus_tag="PK576_01465"
     CDS             18503..19471
                     /locus_tag="PK576_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60289.1"
                     /translation="MSLPLLEVENLAVVFRQEGVETVPVRSVSFTIRAGERVAIVGES
                     GCGKSLTAMSLTRLPPTDRARLSGRVRFDGTDTLALNHAGLARVRGRGIAYVFQDPSG
                     SLNPVMRVRDQIAECLPDLRRADRADAVVRLLTQTGLADPLRAARAYPCELSGGQQQR
                     VMLAMALAARPKLLVADEPTTALDVTTQRQVLDLIDTLADACGMAVLLITHNLGIVAG
                     RMSQVHVMYAGQIIESGPVTQVLTRPRHPYTRGLLAAVPLLDGPRDAPLQDIPGRVPS
                     PDVWPPGCAFAPRCPEASERCSAQSPPKTGDATHTCRCWLEEAPCS"
     gene            19462..20265
                     /locus_tag="PK576_01470"
     CDS             19462..20265
                     /locus_tag="PK576_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE60290.1"
                     /translation="MLLDVRDLHVLYRTPGRQPVAAVRGVTFSLDTGESLGLVGESGC
                     GKSSLARALIGIQPPEAGTIRFEGQDIARLTPPAFARYRRAVQMIFQDAAGSLNPRMT
                     VRQTLEEVLRVHAMRPAARIPDRIRELLDRVGLPETVLNAFPREISGGQCQRVSFARC
                     LALEPRLILADEPVSALDVSVQARILNRLRSLQKELNLAIILISHDLAVVRNICDRVV
                     IMRAGEFVECGTANEVLDTPQHPYTRELLAAVPDIARSLRSKVPPQPVT"
     gene            20287..20853
                     /locus_tag="PK576_01475"
     CDS             20287..20853
                     /locus_tag="PK576_01475"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE60291.1"
                     /translation="MPPPLILGSASPRRSKILQALGCVFTVSTVDTKELHDDFDPIRA
                     VTFNALEKHRACRLRHPESALITADTLVWFEGRLIGKPADFDEAAELLRSFSGRTQIV
                     YTGVALSLPGQEPELRVEASSVRFRELTAAVIADYLHRTRPLDRAGAYDIDENGDLLI
                     AEVCGSYTNVMGLPEAPVRDWLRAHGLI"
     gene            complement(20846..21220)
                     /locus_tag="PK576_01480"
     CDS             complement(20846..21220)
                     /locus_tag="PK576_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013459605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOE60292.1"
                     /translation="MRKISTETAPAVIGPYAQAVEANGFLFCSGQLAVNPASGMIEMA
                     GAAEQTMQVLLNLRAVLEAAGSGLDKVVKTTVFLTDMGDFPAMNKVYAEMFGSHTPAR
                     STVQVTRLPKCAKVEIEAIAVI"
     gene            complement(21247..23178)
                     /locus_tag="PK576_01485"
     CDS             complement(21247..23178)
                     /locus_tag="PK576_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOE60293.1"
                     /translation="MTRRLTMWMVMAALTAGAAPVAVIHGYQSVSEGERRFAASLARH
                     VERWYREVGVEAVLTDDRDLSRSLASARVAVLVYLSQPNAMQLAALKAFAGRGGSMIV
                     CYSSSAELAALLGMETVGYQKGSTDGRWSMMRFSAQRPPGTPERILQTSRNLFRVQPV
                     AGRSQVLAWWHDRQGRQTGEAAWLMSPNGYWMTHVLLADGDADAKGRLLLALAAARDP
                     SLWRPAAARVLDRARGIGGAGGAEGLRMNAERLTDPARRTRALNAARGALRSEAETVR
                     ALAAGKGYEAWLAAQDLRARMYEAYGAAQPVRKGEFRGVWEHSGMGLYPGDWSRTCRL
                     LKDAGISDLFVNVAGAGFAHYASGVLPRSRVFDEQGDQLAACLAAAKPLGLRVHAWLL
                     CFSTEGATNDRIGIFAKRGWLLTHPDGTPRPWLDPAVPAVREYLLKAVREMATAYKTD
                     GVHLDFVRYPDYASSLGPTVRTRFEKATGRSAADWPEDVKSGALRASFIRWRAERVTD
                     LVQASRRTLRRDAPGKLLTAAVFGKYPSCLDAVGQDWESWINIGLVDYVMPMNYTEDL
                     SRFNEWLSQQTRLRKQTMRVLPGIGVTAAESRLDAAQVIDQIRAVRRAGCPGYLFFDL
                     DTTLHQEILPVLRLGVTAP"
     gene            complement(23211..24518)
                     /gene="hisD"
                     /locus_tag="PK576_01490"
     CDS             complement(23211..24518)
                     /gene="hisD"
                     /locus_tag="PK576_01490"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOE60294.1"
                     /translation="MRATIAQWSLTRPTPKVERFLARPAFSPEAETAAAAVLAEVRAH
                     GNRAVLAAARRFDGAALRVSEMRVTDAELKAATREVPARVRRAVNDAHARVRRYAEAS
                     LREAWHMPLPRGGFTGEFFSPMGRVGVYVPGGTAPLASTAVMTVTLARTAGVREIVAC
                     TPCGRDKRVNPVLLHALRVAGATEVYRVGGIQSIGLMAFGTATVRPVQKIVGPGNAYV
                     TAAKRQVYGIVALDQVAGPSEIAILADDSAAPEWVAADLLSQAEHGSGHEKALLVTDS
                     LVLARAVRDALTAQTRLLSRAACIERVLAVGGILAVVVPDLDQGMELVNRFAPEHFEI
                     MTRDARLRLNQVRAAGAVFVGPWTPEAAGDFVAGPSHVLPTGGAARMFNGLTADDFRR
                     RHSFVEFTREDLIETRRTIETFAEVEGLDAHGRSARIRFEERC"
     assembly_gap    24688..24787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24788..25332)
                     /locus_tag="PK576_01495"
     CDS             complement(<24788..25332)
                     /locus_tag="PK576_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60295.1"
                     /translation="MRNGLMQMAMVLMAVGLARAEMVSLVQITDMRGQTGFEILNREE
                     YTALQKEIREETAAYTVAAAESKKQWDANKENKLPFPGNKIKPRSAKKMGADFPNREK
                     AETRKARLEERINEKQLEEMDKESRRKKMMKDEDLAKEASREKAFEDAFAMISKRMAE
                     KLGRPVPTFGVTFVEPAKDEPA"
     gene            complement(25380..27431)
                     /locus_tag="PK576_01500"
     CDS             complement(25380..27431)
                     /locus_tag="PK576_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE60296.1"
                     /translation="MSLGFIKKIADKLLGQKQQSQKKPAAKTGRGAKNPKREPSRNGR
                     RSRPQGGRAKPQASKEGSAGRSSRGASGREGARKADAGRGKPRRESSGRGSETRPRRE
                     SGPPAVAAAARSVARPPSKEPRRGRGRGGGERSDPNTRRPGGAVGDSERAARIAEHAS
                     WSPESFSVEPAEGKKRFHDFDLPGEILHAVADLGFQYCTPIQALSLEHALAGKNVAGK
                     AQTGTGKTAAFLVAILTRYLRSPESRQQKGGAPRALVIAPTRELVIQICKDAEALGKY
                     CGLRSLAVYGGMDYDRQKREVSDAPVDLLVATPGRLLDFVRSRVIDLSKVDTLVIDEA
                     DRMLDMGFIPDVRSIIARLPPKNRRSTMLYSATLTEEVMRLALQWMEAPVKVEVESEQ
                     VTTAAVKQVVYVVTVNEKFTVLYNHIQQYPDSRMLIFCNRRNTTEDVADSLIRRGIRC
                     EMLSGDVNQNRRLRVLEDFREGRVRIVVATDVAGRGLHVDDIGFVVNFDFPYEPEDYV
                     HRIGRTGRAGASGVAISFADEDESFIIPEIEKYIGEELKCTVLAPDDPLLAKLPPKPA
                     GRSRRASPSESANSRRPAGAQRERAGEGGTAVRGDARPRRSEPSPAAVPANAAQPKEP
                     AAAKPEPKPEPRKPEKPRPAPARRAETPARSAVTAAPRLAVAQRRPRFSEEWVPGQQS
                     K"
     gene            complement(27515..27907)
                     /gene="rpsI"
                     /locus_tag="PK576_01505"
     CDS             complement(27515..27907)
                     /gene="rpsI"
                     /locus_tag="PK576_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE60297.1"
                     /translation="MAKEIAAGTGRRKTAIARVRLVPGDGKWFVNDVAIDQYIPSEAL
                     RQYLSQPLELTGMQGKYDVFVSAKGGGISGQSGAIRHGLSRALVASDATLRDVLKKAG
                     CLTRDARMKERKKPGQPGARKRFQFSKR"
     gene            complement(27970..28401)
                     /gene="rplM"
                     /locus_tag="PK576_01510"
     CDS             complement(27970..28401)
                     /gene="rplM"
                     /locus_tag="PK576_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE60298.1"
                     /translation="MTKTTKPKDPGPNRSWHLVDATGKPLGRLAVNIANTLRGKNRPT
                     FDPSVDTGDFVIVVNAEKVLLTGRKEEQKEYQRYSGWRGGLKRFTAKTMLEKHPDRVI
                     KEAVWGMLPKNTLSRKMMTRVKVYRGAEHPHEAQMPVAATF"
     gene            complement(28488..29033)
                     /locus_tag="PK576_01515"
     CDS             complement(28488..29033)
                     /locus_tag="PK576_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60299.1"
                     /translation="MSLSNWCYLLGLFCLSLGVTIVLCPGPAARVFRAFPRNRAAGIV
                     LSTLAWIWAGAALWTMGLDLLMPFKRYIPFAVLVCIPLTWFWLDNLLPCRALGGLLTL
                     FPYDLLHTARTHPSPWRLVVVAVAYLCIIKGMVLLLYPWKLRQAIDWLTGRPGLFRAA
                     GFLQGLMGALLAALGASVLRV"
     gene            complement(29102..29863)
                     /locus_tag="PK576_01520"
     CDS             complement(29102..29863)
                     /locus_tag="PK576_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE60300.1"
                     /translation="MRLLLHNIQYGTGRLRRFAWVETLLRTTAHFPNITRFVRHMNPD
                     IVGLVEVDAGSYRARHRNQAEELAADLSLYHCCRVKYPDEGMGRRLPVFNKQANAVLV
                     REPILRTRFHDFETGFKSLAIEVELENVTFFLVHLALGIRARQRQLSDLYDLITGCDK
                     PRLVAGDFNTLSGVWEMRMFLRATGLRSANTRHAPTFPSWAPCRELDFICYDPSIITP
                     VRFKIPRVRLSDHLPLMFDFEIGGGRIRAPGDGGH"
     gene            complement(29871..31670)
                     /locus_tag="PK576_01525"
     CDS             complement(29871..31670)
                     /locus_tag="PK576_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE60301.1"
                     /translation="MKKISLALFPVLALALFAAAWLWLFCRIPVPAGHMAIITRKTGH
                     PLPPGQILAGPGEKGVQRDPLPEGRHFRDPVTHSWRIVPLVSIPVGKVGVVTAKVGRE
                     LPPGEILAADKEGKGVWKDVLGPGTYRLNPEGYEITQLDAVSIPIGYVGIVTSQSGEP
                     VKTGEFAGPGQQGVMKDVLQPGLYYVNPRAYQVDVLEIGMNQVSINGRSGSVVLTKSQ
                     ISNASSALDELHATTIQKQQQKRADYIGRNSNLLAQNEVAAALGKSREILGKALSRKV
                     SAASPRESKAYAMEAPGGRRRAPETEDLLADTDGPAAMRQAVVPESVAFGINRFVEFP
                     SRDGFQILLDMTVEFELLPEDISRIYMLYGDLPAVVEKIILPQVLSISRIRGSSYKAR
                     DFIDGEGRQLFQKELTAELTRVMGEKHIVIHNAIIRHVEVPGEILTPIQDSSLAKEQD
                     LTNKARQDTARKQAELNTETAMIEQFKKQVEQETEKLVATTVARTRSEVANIQAGTAL
                     AVAATNLLKAAVQAQITRVQGEARVRADYLTANEKALGEQLRAGVFKDPATLADLTFV
                     DKLNPDAGIRILHAGEGTLWTDLKSLAPSLPVK"
     gene            complement(31667..33268)
                     /locus_tag="PK576_01530"
     CDS             complement(31667..33268)
                     /locus_tag="PK576_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE60302.1"
                     /translation="MQEDAEIMPPPLENRRSAPSRRTPSRRAALSSLSLAPLLLMLAL
                     ILGPIWFWFWWRIEPGNGKMAVLFRKTGKNLPVDRIIADEPGYKGIQLEVLPEGRFFR
                     NPYTWGWQIVRVTDIPAGKFGVLVRKYGKDLPGGEILAPDEGYKGIVRDVLGTGKHRI
                     NPYAYEVRLFDDIKIAPGHVGVVTALCGRDILSGIDGSDVVREKGFLAAPGCKGVQSE
                     VLKEGTHRLNPFLYAVSIVNIQSQRFEFSGEEAIMFLTLDGFPVTVEGTLEFNLNIDK
                     VALLTHEVGDMDDIRQKLILPSARGFFRIEGSKKSATEFIVGESRQAFQDSLEKYLRG
                     ICANWGVSLNSVLIRDIIAPQQVAGIIRDRELANQESLKFEQQIVQARSQADLEREKA
                     LAIQNSAKVTAETERIRQTIAAEQVRIEQTLAARTELSVAEIQLEVARADAEALLQLA
                     EADRRVIEAQNRANADVLKQQVAVYRDEADYVRARLYEKTAPKIQGVLTSDDGQGLFG
                     LPVGAKGGPAAPAAGTVKPAKGDTP"
     gene            33678..35183
                     /locus_tag="PK576_01535"
     CDS             33678..35183
                     /locus_tag="PK576_01535"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOE60303.1"
                     /translation="MTTSSAITPHAVAAHLGKPPAQFTKADIIRFIQDRGIQMVNFMY
                     PGGDGRLKTLNFVINDLAYLDAILTCGERVDGSSLFSFIEASGSDLYVVPRFRTAFLD
                     PFTEIPTLTLLCSFFNKEGRPLENSPDYTLHKACKAFTDITGLEFQAMGELEYYVVAD
                     DVALFHAVDDQRGYHESGPYAKFNAFRSHCMLTIAQCGGLVKYGHSEVGNFTQNGKIY
                     EQNEIEFLPTCAEDAADQLVVAKWIIRNLSRQRGLNVTFAPKITVGKAGSGLHIHMRI
                     MKDGRNRMLADGALSDTARKVIAGLMTLAPSITAFGNKNPTSYFRLVPHQEAPTSVCW
                     GDRNRSVLVRVPLGWTAENDMSAVANPLETASVRDTSDKQTVEMRSPDGSADVYQLLA
                     SLAVAARHGFEMPDALDVAAKTYVDVNIHKTGNESIAASLSQLPGCCVDSAASLERQR
                     AVFEAHDVFSPGMIDGVVAALRAYDDKNLRAELMKSPERLAELVERHFNCG"
     gene            35397..36041
                     /locus_tag="PK576_01540"
     CDS             35397..36041
                     /locus_tag="PK576_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60304.1"
                     /translation="MNEENEKPVKTREELAAIAYLDKLRRGVGETMPTELPLACVVKV
                     VAYKEIPKANEKYALVTVRGPIGITWKMCVNRHYLKKGMNALFVSKGAAMPEEERFRN
                     PEAAKVKLRVFKFGPGIKERRLLPIVSRNIYLFNCGLLYPLDDFPELQGVRAGMVCAV
                     KLRIDRLKELHERVAAPKPKQKKKPAPAPKAESDGDDSVLAKVRRYYIRVWRAR"
     gene            complement(36060..36293)
                     /locus_tag="PK576_01545"
     CDS             complement(36060..36293)
                     /locus_tag="PK576_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60305.1"
                     /translation="MSDRGDGRQARRTDRDIRDGTYAPDAASLATACHVKVWRAYLLK
                     LEVRRDVPDRRKRAWCLTLRGTVLPPHKLRGFA"
     gene            complement(36286..38787)
                     /locus_tag="PK576_01550"
     CDS             complement(36286..38787)
                     /locus_tag="PK576_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60306.1"
                     /translation="MCWKRWCSTAVVWAVAGAAGAIFAGVRAPSGGPMVTGQYAFVSG
                     IYPHLAMYNGGGECGIGAVVPWAGRLWTLTYSPHSPTGSDDKLYEITPDLVSVVRSES
                     IGGTPANRMIHPESNQLFIGPYAIGADRTVRTIPYSVMYGRPTGNARHLTDPEGKIYY
                     ASMEEGFYEVDVRTLAVTELWRDEQLAKAKRTPGDNPRFAKLPGYHGKGLYSGQGLLI
                     YANNGDQARQAFVDPSAPSGCLAVWDGKAAAWTVVRRNQFTDVTGPGGILGNPNPETD
                     PVWAIGWDHRSLLLTVLDCGAWHTYRLPKGSHSYDGGHGWNTEWPRIREIGGKSFLMT
                     MHGTFWRFPKTFSAHDSAGIAPLSNYLKVVADFCRWGDWLVLACDDSAKSEFLNKRRA
                     KGTIRGPGQSHSNLWFVNPERLDAFGPAIGRGAVWLGESVSAGVPSDPYLFSGYAVRS
                     LGLAHGSAEPVRFTLETDRAGKGVWKAVRTVEAAPGRLTALTFAERERGAWVRLVPQR
                     DAADVYACFQYANPDRRSDRPDARCAGLASLRDPRAYGGIVYARGGDKRVLGLAAVTA
                     GPDGVRETGYYELDADLNLTRKEDAAAWKVARTDYPMSGTGLVVEAASALITDDAGRR
                     WRFPKADPAYAAPGLFGATRLCREVCTERDLLNVCGIFYELPAENAGGFSKVRAVATH
                     CGQVHDYCSYRGLLVMTGIRENAATSRHLIRSGDGRAAVWVGSVDDLWAFGKARGEGG
                     PWKDSVTAAGVPSDPYLMTGFDRKRLALSHAAAGIVTVTLELDLTGTGVWRVYDTYRV
                     APGQTTRVVLDDARAYWLRVTADRACTATAWLTYE"
     gene            complement(38882..>39321)
                     /locus_tag="PK576_01555"
     CDS             complement(38882..>39321)
                     /locus_tag="PK576_01555"
                     /inference="COORDINATES: protein motif:HMM:NF028447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HOE60307.1"
                     /translation="GLWHDWKTGELVTGPCKRAVKVTPEWGGSLFVRAGAVIPMWEKK
                     AHVDKGWDETVELHVWPGADGKGDLYEDDGVSLEYRSGKGALTTCAFKDGALTVGPRT
                     GTYSGMPATRKMKAVFHKANSLETVTVELGDVGTAGTGPVRCP"
BASE COUNT         7334 a        13092 c        11511 g         7284 t
ORIGIN      
        1 aggcgtgccg cggcatgctc tggtggaacg ccgtatcgaa gaccgccacg ttcggtgtgt
       61 tcgggaaaac cgtttcacag gccaccacgc cgtccagatt ggccggatta tgtaacgggg
      121 cgaggtccgc gcaacgtttg atgctggttt tgacatcttc ggtgatcagc accgagtctg
      181 agaactcctc ggcgccgtgg accacgcggt ggccgatcgc gttgacctct ttcaatgact
      241 ttatgacgcc cgtctcagga tcggtaagca tcttgcaggc catcgccaac gccttgccat
      301 ggttttccgc cgcgatgcct tgcgttattt taggctcgtc gttacgctgg tacaccaagt
      361 tggcgttggg cgtgccgaca cgctcgacca ggcccttggc gagggcggtc tttgtagaca
      421 cgttgaacag ggtgaacttg agcgaggagc tgccggagtt tataacaaga atcttgtcga
      481 ttttttttgc tgccatgata agtccttttt tctctctatt cagtgatcat tggggtaaat
      541 caaacagtca gcggcacact gacaaggcac gccgaagtat gccatattat tctgcacgtc
      601 ttggccgggg tttcaagaac aaactgctcc aaacaaacgc ttccaatcga ttgagtgttg
      661 acttttccgc actacgggct aacctagcgt gcgtgagaac ttgtgatcgc agaggtttgc
      721 ggccgctttc caaagagggg ggagtaatac gatgggtacc aatagaagac gttttctgaa
      781 acaagccgcc gtcgcaggaa tgcctttcat tcttccgtcc cacatttggg cggctccggt
      841 caaacctaat gatcggatga ctcttgcgtt catcggaaca gggaatcaat tttcgacgct
      901 ggtttcgccg ttcctgtatg agccggtgca ggcattggtt gcgtgcgact gcgacacgtt
      961 tctgcgggaa tctgcacaac aacgcatcaa tgagttctac aaaggcaaac ccgagaaagg
     1021 ttcgccctgc tgcgaggcga ttgcggactt tcaggaggtg atcgcgcgca aggatatcga
     1081 tctcatttgt gtggcgaccc cgaaccattg gcacgcatac atcacgattg ccgcactgaa
     1141 agccggtaaa gacgtgtatt gcgagaagcc cctgacgcat accgtgcagg aggctgttca
     1201 agttatgaaa acggtccgag agaccggacg cattttacaa accggctcga tgcagcggtc
     1261 gatgaacgaa tttcgcgttg cctgtgagct tgtccggaat ggttgcatcg gtaaaatttc
     1321 tcacgttgat gtgcatttcg atgagcccgg gcggccctac gatatgaagg aggaacctgt
     1381 cgagccgggg ttggattgga accgatgggt gggccccgcg cggatggtcc cgttcaatcg
     1441 cgccgtcatc tttcgtccgg cacctctcaa accctatgcc agaaggtggc gggaattcaa
     1501 agagttttgc gggggctaca tcgccaattg gggctcgcac catatcgaca tcgctcagtg
     1561 ggggctggat atggatgctt cgggtcccgt tacctatatc ccgccggaaa aaccagatgc
     1621 gcaatggggc gccgaaatgg tttatgcaaa tggtgtccga atgtttcata agccggggtt
     1681 ttcccaagga gtaacttttt acggatcgga cgggaaaatc agtgtctacc gcggcatttt
     1741 ccattttgag cgggacggga aggtgatcag ccatttctgg aaaaagggcg atccctcttc
     1801 ctgcggggca gaagtcgcaa aagcagaaag agaatactta aaggatgcaa aagtaaaact
     1861 ttaccgtagc cgtagtcacg tagaaaactt cattaagagt gtcaaaacac gccagccgac
     1921 aatttcgaat gcagaggtcg gaggccatac cgcgatctgt tgcaatctgc tgagcgaggc
     1981 ttattaccat cgccaacggc tgcaatggga tcccgttgca ttgaaacttg ttgagggcga
     2041 taacaaatgg acagcacgcg agtaccgtga ttggggcggt ccccgctctc cggcgtgacg
     2101 acgctgatag gcgggcgcta tatgcgcccc atttatgcgt ttgcactcat cgcccacagc
     2161 accccctgaa tgaggtgggt gcggaaagcc ggatcctgat agtgcgtttt caaatgcccg
     2221 agagccgtgt aaaagacctg cccggcaccg tgacgacggc accacgcgag cgggaaactg
     2281 tcgcttttga aggtgtcgag ccgcttgtca cgcggctttt tttgcaagct ggcgacgtcg
     2341 acctcgaaaa gaacgtggat gtcaggcgtg cagtcacaga catagggttc atcggtccac
     2401 gaccaagtcg cgtccagatg tgcggtggcc ggatggcggc gatcgctcac ccgtatggtg
     2461 aaaggctgga gcggcgggtg ccacaggaag cgtccgccca gcatgcgccg gaacgcttcg
     2521 gacttgcgct cggagccggt ggcggagtgt agcccgacaa aagcaccgcc gcagtaaacg
     2581 tagtcgagca acgcgccgcg ctggtcatcc gtggtgaacg cctcgttgtt cgtgttggcg
     2641 aagatgatcg ccttcagccc cttaaggcgc gcctcagtga acagcgtggg gtcatccgag
     2701 gcctcggtct tcagaccgcg ctccgcgcaa agctcgcgca gcgcagccac gctggtcgcg
     2761 atgttgtcgt gtacataacc cttgccgtcg ggcgtgtagt tccgtgtgta gatcaagacg
     2821 tccatttgat aaatccgctc ccttcgcaag ctgtggcagg atcaccccaa aagatggcgc
     2881 ccattatacg gggagtgtgg aaagagtcaa cttgtgcaag cacaaaaatg gtaatcggtt
     2941 agtaacaggg gtgtggtgcg ccaaacgaag ttcggcatgc ggggagagaa gcggacggga
     3001 acttgggagg cccgccgtcg tccgcacccg tgggggcgcg gtagcgacac tgaaccggac
     3061 ggcgaggcgg cgcgacgcgg cggcgggagt cagaccgccc cacagtactc tgagacggga
     3121 gagccggtca cacggggaag gggcggcggg gctgcgaagc ccgcaaaggg aacagaaaag
     3181 gacagatatg attgattgct gccgataagt cgttacactt tgggcttgaa caggtgaaaa
     3241 agaggttaca atgtcatgcc cggaaaacct ttgatccatc cgtttttcga tggcaacgga
     3301 cgcgtggcgc ggctggtcgc gaatctgcct gtgctacgtg ccggttatcc gcccgtcacc
     3361 gttccgctgg agcgtcgcgg cgattacatc acccctcgtg gcatgggcgt cccgcccatg
     3421 aagcaaccac gggcaagatg cccgtgccac acccctcgcg gcatgggcgt cccgcccatg
     3481 aagcaaccac gggcaagatg cccgtgccac acttgcaggc aaggggtttt gcaaggctct
     3541 cccaatgttt atggggctgt gctaaaaacc atcgcatccc ttttcaccag aaacgcacgg
     3601 tcttcgcgtc ttcctatcac gatctcaata ctcgttacgg cggatgtctt gcacttcgtt
     3661 gctgaaaatg ttgatgcgaa tgtagcgggt tacgttgtcc ttgacggcgc gtgcgtagat
     3721 aaatttctgt ccctctttga tcgaagcggg gaacgtcact ttgccccgct tgtcgacctt
     3781 ggcggtgatg tcggtctcca cgccgcccgg ctgcttgtag tagaggcgcg aacccggttt
     3841 gacattgaaa gtgaccgtga tggtcgccga ggccgagaca tcggtggagt cgaacgtgac
     3901 gtccacgcct gcggcacgca tgttcagcac ttggcgccgg ttggcggtca gcagcgcgtc
     3961 gggattgtcg aagagcagtt gcggatgcag gcggtcgagg tcgctcagcc gcgccacgcg
     4021 gaagcgatat tcgggatagg agacggtgcc gctcttggtg accggcccga cagccaccgc
     4081 gcggatcgcg ttgtccacgc gatcctgatc ggcggggcgc gtcaacatct tgaagcggca
     4141 ggtgcagggg gtggctccgg ttgcggattg tcgagcttgc gcccggtgct tccgtgcctc
     4201 ctccagtttg cggttgcact ccgcgaagac ctgcttctcg cgcccgacca gtttgttgcg
     4261 ggataacgct tttatgaccc gcgtcacggt tccaacctga caaccgccgt tcccatggag
     4321 tccatgtcga gcgacaacac gccgcccgcc cttgtcacgc cgctgccgaa cactggtgcg
     4381 cacgtcgtat aggccccttc aatttccacc tgtcccacgc gtcgatcagg gtgcgtgtta
     4441 cagacgagca cgtagacttt ctttccgtct ttccatgtgc gcacacgcgt ccgcggcggc
     4501 attttacgca catccgggcc aggctccaac agaagaatgg ggatgagatc gcgcacctcg
     4561 accgcgaccg aacatgtcgt acggaagttt tcctccaatt ccgccccggc tttattcatc
     4621 atctgtccgt aggaatagaa caaaagcccg ttcgcccctt cggcgatttg ttgccaagtc
     4681 atgctggaga gttcgcgccg ggtagggaaa cgcagttcag gttgttcgct tttgtcccac
     4741 ttccaagcaa aggcctgcgg aacctgccac atcggacgca ggccaaacac gctctctcgg
     4801 atggtccgcg ccgagtcgcc cgcgcgccat acggcaccgg cgtccggagg caggaccgtg
     4861 ttgacgcggt ccgatcccgg tatgggataa ggatccgatc ccacgacatc cgtacaatcg
     4921 atgaatacgc gcgcggtcac aggatggttc aggcagatga acgtgggatg gtcgggatca
     4981 agctgtttaa gaagagaata acggtcttcc acccgatcgg cgtattcatg cggaatctcg
     5041 tcgcaggtgt accaggccag cagcgcggga tggttgcggc agcgttcaac aacgcgtttc
     5101 acgtagtttg tctcgtcggt ccgcgtcttg acttccggcg gcgtaaagcc aatcagcgga
     5161 ccgccgacag gacgcacata gatgtctttg acgctcgcca cgacacgtaa cccatgacgc
     5221 cagtaacgat ccaatatctc aggcgtcatc tgcttttcat atgtctgcag gcagttgaac
     5281 ggccccgaag cgtaacgctc caaagagttg gacttcgcca gcgtgttctc gctccaatac
     5341 atgcccagag ggaagaagcg tttcccctcg acgagtgtgc gaccgtaacg atcgaagcag
     5401 acacgccgcg gcggcaacgt gtcaacacgt tcgaacacaa gggttttctc gcccaacacc
     5461 tctccatttt ccagcttcag agtcgccacc aacggatgtt gccccatggc gaacgatgtg
     5521 acggggattg atgccacgaa ctgtttggct tccaccgcgc gcattgcatc ctgacgccgt
     5581 gcaccatcgg aagccctgta tgcaagttcc gtcaccagtc ctttctcccc caagtatttg
     5641 tcaggcaact caagaccggc acggacgtgt acgatccccg tggcagccat atcgcgataa
     5701 caggacgttg tcagtgtctt gacggcagct ttcttcggat ccgcaaacgt gtccacgcga
     5761 atgtcgtcga aagcgacgcg accggttccg ccggcatgga cataaagctg aagctggaca
     5821 gccgtcgctt cctcgggcag acgttcggac ggcattcgaa tcgtcatctc gtaccacccg
     5881 tcggcgtccg gcttgagccg cttgtctccg agtttcatca ccgtgccgcc cgcgccgccc
     5941 agccacttgt ccttgcccgt ccagcaaatc gtgcacccca gccgcgcccc cgtgatcttt
     6001 tccggtttca ccttgaacgt tgcgacgaag tataagcccg gccgcccacc ggggatggtg
     6061 aaactgttca cattgtagtt ctttggatcg tcgttggtcc agacaagcgc ccgcgagccg
     6121 cccgtgcccg cgccctcctc gatcttccag ttggcatccc gtttccagcc gcggatttcc
     6181 tccgccgttt cgaaagccgt ctgatagtag ggctcccccg ccaacgcaca ccacgcgccg
     6241 accagagacg ccatgaacat ctgccaccca ctctttttca tgccgaggtt tttcttcatt
     6301 tgtttttgcc tttcgttagc acgttcacat gtgaccgctc gaatctgcgg cgggcgatac
     6361 ggcgagacgc ccgaagaacg gcagacgcgt gagcggcgtg gcctgattcc cgtccgtttc
     6421 catgtttaat ctattatgcg gtcgggggac gaaagcagtc aaccccgaac ccccccggaa
     6481 cgtgttgaac ggtgagggcg gcaccccctt ccatgcggga caaaaggtgt ggcgcacgga
     6541 atccgacctg cggccggaac cgcggatacg atgcttgcac ccgttccggc catccgttat
     6601 tctcttatcg aatccgacac gtttcagaaa ggacacgact catgacacgc accctccgtt
     6661 tgatcacgat ctgttcgcta ctcgccgcat gcgcgggcct gctgtcggcc gcacccgagg
     6721 ccgaagccta ccgcatccgc cgcggcatcc cgaacagccg gtactttctc cagaacgaca
     6781 ccgtgggcaa ccagtatctc tttttcatcg gccattccgt gcttgcggga accggactga
     6841 aaaactcggg gctgcgttat tccgcgtgta tgaccaaagc cctcaaacgg cacttcccga
     6901 acgcctcgcc ccgcgagacg cgccacatgt accccggcgg cagctggttt gcccagtacc
     6961 gttgtagccg cggccagccg gttttcggtg aggtgatttg cagtggccac ctcgcgattc
     7021 tggatttcgc ggctgaggat cgaagcgctg atctcgaaca ggtcaaaacc tcccttgagg
     7081 gcctgatccg tcagatcacg ctctaccgcg ccacgcacag cacgatcctc gtatacacgc
     7141 taacccccga gatgctggcc gactaccgtg cgggccgcac gcccgcgtac atcgaggtct
     7201 gcgagcggat cgcggaccac tacggcattc cgagcctgaa cctggcccag gtcgcggccc
     7261 gcgcgatcct ttccggcacg atctctgccg aggctttctc cgctgacggc atccatccca
     7321 cggacgcggg tgcgcgtctg tgggccgagg cgatcaatgc ctttgtcgac gcgctcatgg
     7381 ccgacggtcc ggttcccgaa aaaccgcaga gggtgaccct ccccgcgccc ctgttccccg
     7441 gaacggatga ccgtggacaa atcatcgcgt atgaacaccc ggaggtacgc accaccggtt
     7501 cttggcaacc gggccgggag agtccgatca agcccttccg ccatgtgctg gtttcccgcg
     7561 agcccggcac ccggctgacg ctccggttcc agggttcgga aatcggcctc atcgatgtcg
     7621 ccgctccgga cggcgcggat ctcgaatacg cggtggacgg cggcgcgttc cgcaccctcg
     7681 cggcaccgaa gaacacagac aagcccgtct tgcgcgcgct cccgctcgca cgcggcttga
     7741 cgcgggatgc cgaacatgag gtcgtcatcc gcaccgcttc ggacggcacg gcgcgcatcg
     7801 gcggactgct ggtcaacggc agcgtgaaga acccccacgc cggaatgagc acgctcgaac
     7861 ggatcgacgc gatctacgcc gcgatggacc cgatcaacta cacgccgcct gcaaaccgct
     7921 ttgcccacat cccgaaaacc atggccaaac tgcgcggggg cggcgaattg cgtatggttc
     7981 tgctcggtga cagcattatg ggcaacacct cgggctcctc gttcgaactg ctgctgatgc
     8041 gcaacgcgcc cgcctgcaaa atcatcaaaa tcgcctccct gcgcagttcc accgggtgca
     8101 cctactatca gcacgagaac cgcgtacagc agtatgtgct gcgccacaac cccgatctgt
     8161 tgatgatcgg cggtatctcc aacggcggcg atgccgaggc cgtgcgttcc gtgatccgtc
     8221 aggtccgggc ctcgaagccg gatacggagg tgctgctgct cacgcccgtt ttcggcgcca
     8281 tgcgtgatga gcacatccgg accttcaccc gggagatcga caccacgacc ccgaacttcc
     8341 ggcaccgtct cagcaaggtg gcggaagaag agcgctgcgc cttcttcgat atgaccggcc
     8401 cctggtggga atacatccaa aagagcggca agacctacgg gtggttcatg ggcgacgccg
     8461 tgcatgccaa tgcgcgcggt tgccagatca tcgggcgtct gctggagcgc tggttcaagg
     8521 agtgacgcgg ttggaattgc cggaagggcg cgcgcccgcc cggtatgcgg cttcgcgtgc
     8581 cagacaggcc tccacagctg cgcggacctc ctccagcgtg tggagcgtgt gcagacggcc
     8641 gcgcagatac gaggagcctt tgagcccgtg caggtagcgg aaaagatgcc cccggaaggc
     8701 ggccacccct gcagcgtcca gatccctaac ggaaccggcg cggccctcgc gttcgcaaca
     8761 gcggcgcgcc aattcgcatt cggcttcgag gtgcgcctcc aaaacagacc ggatcgtctc
     8821 aagcgcgcgc cgtggacgac cccgcgccgt ttcgacgggt gacggggtcc tgcccgagac
     8881 gagggcgtcg cgaatatcgc taaaaagcca ggggttaccg atggcggcgc gcgcgaccat
     8941 caccgcatcc acaccggtct cctgcagcat gcgttgggcc gtatacggcg aacccacccc
     9001 gccgttgccg atgaccggta tccgggcgcg gcgtttgacc tcagcaagca gatcgagccg
     9061 cgcctccccg ccgtgtcctt gcgaggtgaa ccgcgcgtgc agcgcaaagg cgtcggcctc
     9121 cgcctcctct gccgcggcca acagctcgaa aaccgtcacc cggcccgggt cgggcccgag
     9181 gcgtgttttc acggtcagag gcaacgtgac cgcgcggcgc atggccttta ggatcgcgtg
     9241 tacgcgttgc ggatccgccg ccagagtcgc gcccgcgccg gtcgcggcga tcttccgcac
     9301 cgggcagccc gcgttgagat caatcgcggc aaagcgtccc gtggcttcca cgcgtgcggc
     9361 ggcctcggcc agaatctcgg gctccgaacc gtagagatgg gccacaaccg gcccttcgtc
     9421 ggcaaacgtc tccagcagat gccatgtttt gtccgaggag tgcaacagac ccgccgcgct
     9481 ggtcatctcc gtataggtca gttccgcacc gtgcgcacgg cacagcgcgc gcatcggtgc
     9541 tccggtgaat ccggccatcg gcgccagaat cagcggcggt accgaacgat cggagaggga
     9601 gaatatgggt agagaaggca taacgtccgc gttgagatgt gtaaggtatc cccatcccgc
     9661 agggttttca acccaacaaa cgtcttttgt ccgtttccga tgatgatgcg caaaaagaac
     9721 cgcttgccgg atttgacccc gaaggccccg cccgcgtata cttgaagccg tcatgcgtga
     9781 agttctcgat gtcttgctcc tctccgtgct gcagggaatt gccgaattcc tgccgatcag
     9841 cagttccggc catctggtgc tcgcccaaag cctgctgggc gtcaacaccc caggcatgcg
     9901 gttggaggtg gtgctgcatc tcggcacact ggtctccatc ctcgtgtatt accggaaccg
     9961 cctcacgcag ttggttctcg ggaccgtgcg gggcgaccgc gaaagctggc gcacagcggg
    10021 acagatcgcg ctttccgcca tgcctgcggt tttcttttat ctgctctgcc acgacaagat
    10081 caacgccttt ttcgagtccc cccgggccgt gggcgggttt ctgatcttca ccggcgtggt
    10141 tttgtacgcg ctgcgctgga tggcgtgcgg ccaaggcaga ctcaccgccg cgcgcgcgct
    10201 gctggtgggg ctggcccagg cgctggcggt cctgccgggc gtcagccgtt ccggcatgac
    10261 gatcgcggcc ggacgcatgg caggcatcgc gccggaccgg gccgcggagt tctcgttcct
    10321 gatgtgcata ccgctcttaa tcggcgcggc cctgatggat ggactgggca tcacgccggc
    10381 ggcaccgaaa gccgatgcgc tgcccgtatg gctgctgttg gcgggtgccg cagtttcggc
    10441 aggcgtgggg tatctcgccc tctctctgct cgtgcgtgtt ttgcgtgcgg gccgcttctg
    10501 gctgttcggc ctctactgca ttgccgcagg tttcgtgaca atcctgctga cctgagtggg
    10561 ggacgcgtca ctgcgcaccg cgcgtccagc gcgcaaaggc cccggacggc gtggacggca
    10621 cggtggccgc gccagtgagc agcatctcgc ctgaaacgat tttgccgtcc aaatccccca
    10681 ccgcctgccc gaggacgttc tccagcacga gcggcgaata cccgggagtg tgcgcgctca
    10741 gcagcacgaa gagcgggtcg ggggcgagca ggctacggca aaggcggagt gtctcgggca
    10801 agtcgcgttc gatcttgtag gtttcgttgc cctgtccccg gccgaaggtg ggcggatcaa
    10861 ggatcaccgc atcgtaacgc cgttcacggc gtacctcccg gcgcaggaag gtgtgcgcgt
    10921 catccgtgat ccagcggatc ggatgcgctt cgagccggtt gagccgcgcg ttgtcccgcg
    10981 cccacgtcac catgcccttc gaggcatcga gatgacagac ctccgcaccg cccaaggcgg
    11041 ctgccagggt cgagcccccg gagtaggcga agagattcag aatccgcacc ggccccggac
    11101 gcgcggcgca tgcgtcccgc acggtgttgc ggagccacag ccactgcgca cgctgttcgg
    11161 gaaacacgcc gaggtggccg aaatccgtgc ccgagagccg gaaacggatc cccgcgatct
    11221 ccacctccca tgcggcgggc aggccactgc ggttgtgcca gcggttccca tcctcacggt
    11281 caaacgcggc gtgcgccgcc gcggtccact ccgcctcgct caggcggggc ttccaaaccg
    11341 cctgggagca gggccgggcg agcgtgtacg cgccgaaacg ttcgagtttc cggccatccc
    11401 cgctgtccag cagttcatat tcatcggcca gacccggact catcttccct ctccgcaact
    11461 ccttgcgccg gtcacgtttt cccccagagc agcaacaacg ccatcgcggc gaaaaagaag
    11521 ccgagcagga ttttgacgac cacgagattc cgcttgctcc agttgatcag cgaggtcagt
    11581 tgcaaccccc ggtggaaaca gataaacacc acggttagcg gcaggacaaa gagcaggttg
    11641 taggtgatca gcaggatcca ggaagtgaaa tcggaacagt cgcgcttgag catatacatc
    11701 aggaccggca cgtaactctg ccccgtgcag acactttcca gcaatgtgac ccccgcgcct
    11761 gtgaccagtc cgcccagcac cggcccgccc acaccgagcc gcgagttcat gaacgcgtgg
    11821 atctggccct tgatcttctt cgggatctgg agcgtcacgt catccggccg ctgcgatttg
    11881 cggaaacgga tggcgtcgcg aaaggagaga atcgccaacg gcaccatggc aagcccgagc
    11941 accacctcga taatccgctt gaccatgacg aactgcggca cttgacggaa cgcgaagaga
    12001 aagcccatac cgagtcccat gtatacgaaa aaggaggcgg tgatgaacga agcgccaacc
    12061 agcaggcggg tgcgtcggtc ggcacgggcg agggcgagca cactcatgaa aaaaatcagg
    12121 gtcgaaatcg cgcaggggtt gaacccgtcc agcaaaccgc cgagcgccac caccgagaag
    12181 gtcagcgccc ccgcccgctc cttcacgatc tccgccgcgc gctcgtcatc caaaatcggt
    12241 gcctgcggca tctgccaacc cggttgctgc cgccgcacca gtgcctcgcc gactcgatcc
    12301 agcagacccg tgacaatcaa atccgcgccc gagaggaaca cgctgtgatc gatcaccagc
    12361 gccacgcggc cgttgtcggt gttgccgcac cgctgctgat aggccacgag caacgggacg
    12421 ctctcggcat ccgtgagatc atgtgaggcc aactcgtaga gcccgtcaag ctgatccgcc
    12481 aattcaggca acgcctcccg tttcacccgc tcgcactctt cgcatcccgg ggaaaagaag
    12541 aaatccaaac gcaccgcatc gtcccggatc tcgggcgccg caaagacgaa cccgaccaga
    12601 caaaacttcg cgacgaccat aagccgcaaa aaccgaatta ctctgaaaag gtctttctgc
    12661 ataggaataa ttataaaata ccgcatatat ggcaaagacc acaacctaaa agtccgaacg
    12721 tcacgcaatc ggttcgtgac aggggggaga gtcatcgtgt acgggtacgg ggtatggcca
    12781 acgttcacgt tctccaaatc gctatcggta tcggtatcgg tatcgctatc gccatcgggt
    12841 tcgaaatcga ctgttgtgcc cacgcagtgg cccgcagcga caagtgtaat gatagcggcg
    12901 attccgattc cgataccgat ggcgatagcg atggcgccgc aatggaaccc tgaaacgcat
    12961 gaccttcgca cacccctgtc acgaaccgat tacgaacgtc acagatcgac atttcgtgct
    13021 ccagtcactc tcaggcgtcc gagacgtccg cccgtactct gttcggctca tgtccctgcc
    13081 agtacggcgg gacgtccccc ccttccctct tcgcctcaca cctccaagcg ccacgtgaac
    13141 tcccgcactc cgtcggcggt ctgttcacgg aagttcatat tcaggatgcg tttggccggc
    13201 gaggcaggca agcggatcag ttcgcaacac tcttccagca agccttgggc gtcgcgcttc
    13261 gccacgcgct cgggtttgat tgctacgccg tccagcgtcg ctccgacaca cgtgatcgcc
    13321 gacggatccg ccaacttccg cccctccacc cattcgcgcc gcacccgaat ccgcacgcac
    13381 acgcccgcgt ccggacgtcc gaactcgaac cggtccccgg gccgcaccgt caccaccgac
    13441 acgggcatcc ggtccagcac ggtgccggag tcatcccacc agcgccagtc atccagacgc
    13501 ctcatgcagg cttcgcgcac ctcgggcgcg ccgcccagca tgcgtatccg cccggcggtc
    13561 cgcgcatcac gcctcaccgc caccacccgt ttctcccgca aggcctcctg ccacccttcc
    13621 caccccggct cgcgcgcgag aaacagggtt cggaacccgg tgagcatccc gctccaccac
    13681 cacgactccc catgcgcgtc ctgcagggac acaaagggga tttcgtgccg gtagcgcatc
    13741 ataaagggca atgcccaaat catgttgtgg caaccgaaat gaaaggtgct gatggcgccg
    13801 taaccgccac gcaggatcga atcgtccagc acgattcgcg cgaactcctc gttgtcacaa
    13861 atttgccaga gcatcatgcc tcccaccgcg tggagcgatc cgacgcactc gtcgcgaaac
    13921 tcccgccagc tcccatcgcg ccgcggccac gccggcaccg gacgccccgg tgccgccaca
    13981 gggtcattga catgggtata tgaacccaac cccggcaggc tcacccgcgt tccgtaggcc
    14041 tcgtccgagg caaagaacgt caccggcaca ccccgttcct ccgcgcaggc ttgtgcggcc
    14101 tccctccagc cggggcgact gtttttcgcg ccccacaagt ggatcttgag ggccttggcc
    14161 agttcaacgg cctcaatgac cgaaccttcg cccggcgctt caaactggat cgtgaacgca
    14221 tgcagatccg cagggatcac ggaccgtctg cgtgaggggc ggtcttgcgg tccggtatcc
    14281 cgcaacccgc ctcccgtacg cgcaaggata tcgcaggcct gataggtcgc cagtgctgac
    14341 gagaccccgc ccggccggtc ggcgaacccg ccgtccgcgt tccagaggga ccggatccag
    14401 tcgcggcaag cctccggccg taccggtttc ccgcccagca gttccagcgt ccggatcgcg
    14461 gcccacgtgt aggagaggtc actgagcccg ccgacctccg gttcgggaca ccaggagaat
    14521 ccgccgtccg gccgttgaca acgccggatc caatcgagcg cggcgggtcg gggggcgatc
    14581 cccagagcct gctgcgcgcg gataccgaaa aacgtgttca ccagatgccc gccgctgccg
    14641 tccgccgcag gcgtgttgtt gaaggtgccg tccggtctgc gccgcgcctc cagatatccg
    14701 ccgaacgcct cggccagcgt ctcgcacggc aaccccagca gtacgcggca caagaggggc
    14761 tgtacctctt gacggaagac cgggtttccg cccttctcat acgccgtcgt gtaccccgag
    14821 accttccgcc accccgcaac ctgtcgggaa aagccctccc ctgccggttc gcccagccaa
    14881 agcagggtct gaatctgttg aaagacgaac tcttttatct cggcccagtg ccgccgcgtc
    14941 tgcgcgtacg gtccgttgat cggatgcgcc cgcctgacat acgctgcggt ttcagcagga
    15001 cggggcggcc gcatccccaa agcatgatac gccccgaccg ccgcatacgt ttccgtcaga
    15061 aaggaatcct cctgatcctc ccatccgtaa ccgccgtcgg agcgtgccaa agtctccaga
    15121 tagcgcgtga ccgcctcgcg gaacgcctcc gtccccccgg cgtccggcga agccgcgcct
    15181 cccgtaaccc agcctgcggc cggagcggcc gcgccgacgg tcttcaaaaa ttttctgcgg
    15241 gtgatccgtt ccatcgttct cccttattgc agccaaatca acataccgtg taatcataat
    15301 tttagacgcg atccccgctt tgtcaacgat gacaccgtcc cgagaaaaac cggaggatac
    15361 cgatcactcc gatccgacaa atccacaacg ggcgagaaca cccgcgttcc cgacaccgca
    15421 gatcgctggt cgcagggaaa agcgtccggt ccttgtccgg cacggggaag cgtgttacag
    15481 taatccgcat gatcaacctt aaacaagctc ttctcacgct gtccggcgca atgtgcgttt
    15541 tcgccctccc ggtgcgggcg gaaaaaatcc tcgtgttctt cggaaccggc ggtgcccacg
    15601 cccaaggcat ctaccgcgcg tcatttgaca cccggaccgg ccgactcgag ccagcggcgc
    15661 tggcggccaa aatcggctct cccggttttt tggccttgca tccggacggg gacaagcttt
    15721 acgctgcggc gaacggcgag ggtggtcaag gcgtgaccgg tttccgcatc ggcggcgacg
    15781 gtgtgctgaa cgccttcacg gccgcgccta caggcgacgg ccaggcgtgt catctggccg
    15841 tacacccctc ccggcgcttg ttgttgaccg cgcagtacgg caccggctcg gtcgcgctct
    15901 ttccgctgga tacggaagga cggctgggcg cggccacaac gcacaaacat ccggggccgg
    15961 gaacaggtgt ggtcgcgcaa cgtcagaaga caccacatcc gcactggtgc ggcttctcgc
    16021 ccgacggccg cttcgcgctg gtgccggacc tcgggctgga cggcatcatg gtttaccaga
    16081 tcgacgcgac tgcgccgaat atcgcacgcc acggcttcat caccgcgccg cgcggcgccg
    16141 gaccgcggca catgcgtttc tccacagacg gccgccggat ccatctgctc aacgagcttt
    16201 cgctgacggt ctcgacctac gcgtgggacg cggaaaccgg aagcgcgcgc ctgctgacca
    16261 ccgtcccgac cctgagcgaa acagagaagg ccggcgaggc gcacaacagt gccgccgaaa
    16321 tcctgacgca cccgaacggc cgtttcctct acgcctccaa ccgcggaaac gacacggtga
    16381 cggtgttccg cgccgatccg gctacaaccg cgccggagag gatccaggtt cagccggtgc
    16441 gcggcgcgtt cccgcgcaac atcaatctct cgccatgcgg caactggcta ctggcagcgg
    16501 gcgcggactc gcacaccatc gcggtgcaca aagttgatcc tgaaacggga cgactgacct
    16561 accagacgcg aagcgtgatt tcggtgcccg caccgatctg catcctcttc gcgcgtgtgc
    16621 cgtaggcgcg gagcgggacc gttgaaaccg tgccggactt cggggtaggg acgcctcccc
    16681 gaggcgtccg aggacgcgga caggcaaacg tctgccagcc tggacgggca gacgctcgtc
    16741 ccttcgtgat ttcatgaagt ttcttgtgtt tcatggttac ggatatttac aaggctttgg
    16801 ttgcgattgc tctttttagg cttataagat cacggctttc gcttgcaaac cgctgatgtc
    16861 ccacctacac tggcaactgt tgaaagcgga gcgcccgacg cgggccgccg cagtttcaga
    16921 ctgaacgttt caggagtgcc tgctttatgt gtccaacccg caacccttct gacccgaaag
    16981 cggcgatcgc cttcacggaa cgccaacagg ggatcgtggc cgccggcatc acggtccttt
    17041 gcgccacgct gatcaccggg ttcgcggtcc tgctgttctg ggcgttgttc gcgttgctct
    17101 cggccgtctc ggtggtgctc accccgctgc tcgtggcgat gatcctcgcg ctgcttttca
    17161 aaccctatta tcagtggctc cacgcgcatt tgcggcgctc gcacctgctg gccctgcccg
    17221 ctttcttcct ctccctcctg attcccgtgg gcggactctt cttttttttc ggctcgctgc
    17281 tggtcaccca actgatggcc ctgtacgatt acctgccgtc gctgattgag cagatccgtg
    17341 acgccctgac gcacggcaac cccgcgctgg aagccttcat caccaagttc ggcctggaaa
    17401 cgaagatccc gctgttgagc gaccccgaat cgtttgtcgc cgccctgttc gaccgggtct
    17461 cgctggacag catgggcggc atggcgctcg cctacggcat ggatgcgatc aagcatctcg
    17521 tctcgctcat cggttggctg gtcgttccgg tctatctgat ctacttcctg accgccaaac
    17581 ccttttccgg caagaatgcc gagaacttcc tgccgtttct gaagcccgcg acacgccgag
    17641 acgtctctta cctcatcgac gagttcttct ccatcatcgt ggccttcttc cgcggtcagg
    17701 taaccgtcgc cttcatccaa gggcttctct tcggtttcgg gttctggatc gtgggcctcc
    17761 cctacggtct gctgatcggt ctcacgctgg ggcttttcaa cctcgtcccc tatcttggca
    17821 acatcatcgg tctggccgtc accctgcccc tggccttttt cggcgagggc ggcagtgggc
    17881 tgcggctcgg tctggtgctg gccgtgttct gcatcgtgca gattctcgac ggttacttca
    17941 tcactccgcg catccaaggc aagaagacgg gactcagcga tccggcggtc atcttctcgt
    18001 tgctcttctg gggcgtggtc ttcaaaggca tcctcggcgt actgctcgcc atccccctca
    18061 gcgcattcat cgtggtgctc tggcgactac tcaaaaccaa gtacatccgg gagctgatct
    18121 aacgcgggcg gagggcagcg caagggtttt gtctgtaaat tcgtccggcg ggtcacggaa
    18181 tctcataaac ctaaaagcca attcgccgta cccgcgcagt ccgatcccgt attcccgacc
    18241 catgaacgaa cacactcccg ctcgccgtcc aaaccgaccg gacacacgct tcccccgaca
    18301 ggccggccag cgacttcgca accgggacaa attgacggtg cgctacagcg aaaactgcgg
    18361 gatcttgagc gtcgtgccgc cctttatcgc ggaaaggtgc gcgtagaagc ccgagaccgt
    18421 cgtgttcccg gaataacgcg ccgcacatcg accgcatttt ctgttttggt tccgcgcccc
    18481 ttcccgttac aatgaaacgc acatgtccct accgcttcta gaggtcgaga atctggccgt
    18541 ggtcttccgt caggagggcg tcgagaccgt gcccgtgcgt tcagtctcgt tcacgatccg
    18601 cgcgggcgag cgtgtcgcga tcgtgggtga gagcggctgc ggcaagagcc tcaccgcgat
    18661 gtccctcacg cgtcttccgc ccacagaccg cgcccggctt tccggacgcg tgcgattcga
    18721 cggcaccgac accctcgccc tgaatcacgc cgggttggcg cgcgtgcgcg gacgcggtat
    18781 cgcttacgtc tttcaggatc cctcgggcag cctaaacccg gtgatgcgcg tgcgtgacca
    18841 aatcgccgag tgtctgccgg atctgcgccg cgccgaccgc gccgatgcgg tagttcggct
    18901 gttgacccaa accggtctgg ccgatccgct ccgcgcggcc cgtgcgtatc cttgcgaact
    18961 cagcggcggg cagcaacagc gcgtgatgct ggccatggcg ctcgcggccc gccccaaact
    19021 gctggtggcg gatgagccga ccaccgcgct ggacgtgacc acgcagcgtc aagtgctcga
    19081 tctgatcgac acactcgccg acgcctgcgg catggcggtc ttgctgatca cccacaacct
    19141 cggcatcgtg gccggccgta tgtcgcaggt ccatgtcatg tatgccggcc aaatcatcga
    19201 gagcgggccc gtgacgcagg tgctgacccg gccccgccac ccctacaccc gcggactgct
    19261 cgccgcggtg ccactgctgg acggtccccg cgacgcgccg ttgcaggaca tcccgggccg
    19321 cgtgccctca cccgatgtct ggccaccggg gtgtgccttt gctccgcgct gccccgaggc
    19381 ttctgaacgc tgttccgcgc aatctccccc gaagaccgga gacgcgacac acacctgccg
    19441 ctgctggctg gaggaggccc catgctctta gacgtacgtg acctccatgt cctctaccgc
    19501 acacccggtc gccagcctgt tgcagcggtg cgcggtgtca cgttctccct cgacaccggc
    19561 gaatcactgg gactggtcgg ggagagcggc tgcggcaaaa gttcgctcgc gcgcgcgttg
    19621 atcggcatcc agccgcccga agcgggaacg atccgtttcg aaggacagga tatcgcccgc
    19681 ctgacgcccc ccgcgttcgc gcggtaccgg cgtgcggttc aaatgatttt tcaggacgcg
    19741 gcgggctcgc tcaatccgcg catgaccgtg cggcagacgc tggaagaggt gttgcgcgtc
    19801 cacgcgatgc ggcctgcggc ccgtattccc gaccgcatcc gcgaactgct ggaccgcgtc
    19861 ggcctgccgg agaccgtgct caatgccttc ccgcgtgaga tcagcggtgg ccaatgccag
    19921 cgcgtcagct tcgcgcgctg cctcgcgctc gagccgcgtt tgatcctggc cgacgaaccg
    19981 gtcagcgcgc tggacgtctc ggtacaggcg cgtatcctga atcgcctgcg gtcactgcag
    20041 aaagaactga atctggcgat cattctgatc tcgcacgatc tggccgtggt ccgcaacatc
    20101 tgcgaccgcg tggtgatcat gcgtgccggc gagttcgtcg aatgcggcac cgcgaacgag
    20161 gtgctcgaca caccgcaaca tccctacacc cgcgaactgc tggctgcggt gcccgacatc
    20221 gcccgttccc tgcggtcaaa ggtccctccg caacctgtaa cctgaaaccc gtattcctcc
    20281 cgtactatgc cccctcccct catcctcggt tccgcctcac cgcgccgcag taaaatcctg
    20341 caagctctgg gctgcgtctt tacggtttcg accgttgaca cgaaagaact tcacgacgac
    20401 ttcgatccaa tccgtgcggt gaccttcaac gcgctggaga aacaccgtgc ctgccgtttg
    20461 cgccatcccg aatcagccct catcaccgca gacacgctgg tctggtttga aggacgtctc
    20521 atcggcaagc cggccgactt cgacgaggcc gccgagcttt tgcgctcttt ctccgggcgc
    20581 acgcagatcg tctacaccgg tgtggcgctc agtctgccgg gtcaggagcc ggaactacgc
    20641 gtcgaagcct cttccgtgcg ttttcgagag ctgactgccg cagtgatcgc cgactacctg
    20701 catcggacac ggccgcttga ccgtgcgggc gcctatgaca tcgacgagaa cggcgacctg
    20761 ttgattgccg aggtctgtgg ctcgtacaca aacgtgatgg ggctaccgga ggcacccgtg
    20821 cgcgactggc tccgggccca cgggctcata tgaccgcgat ggcctcgatc tcgaccttcg
    20881 cgcatttcgg caggcgcgtc acctgtactg tggagcgcgc cggggtgtgg ctgccgaaca
    20941 tctcggcata gaccttgttc atagccggga agtcccccat atcggtcagg aacaccgtgg
    21001 tcttgaccac cttgtcgagc cccgatcccg cagcctccaa gaccgccctc aggttcagca
    21061 acacctgcat ggtctgttcc gcggccccgg ccatctcgat catgcctgag gcaggattga
    21121 ccgccagttg cccggaacag aagaggaacc cgttggcttc caccgcctgc gcatagggtc
    21181 cgatcactgc cggggctgtt tccgtgctga tctttctcat cgctcatccc tccctgatcg
    21241 cgtcgtttac ggtgcggtca cgcccagccg caacaccggc aggatctcct ggtgcagcgt
    21301 ggtgtcgaga tcgaaaaaca gatatcccgg gcacccggcg cggcgcaccg cccggatctg
    21361 atcaatcacc tgcgcggcgt cgagccgact ttccgccgca gtgacgccga taccgggcag
    21421 cacgcgcatc gtttgtttgc gcaggcgcgt ttgctgcgac agccactcgt tgaagcggga
    21481 gagatcctcg gtgtagttca tgggcatgac ataatccacc agcccgatat tgatccacga
    21541 ctcccaatcc tgacccaccg catcgaggca ggagggatat ttgccgaaga ccgccgcagt
    21601 caagagcttg cccggcgcgt cgcgccggag cgtccgccgc gacgcctgca ccagatccgt
    21661 aacccgttcc gcacgccacc ggatgaagga cgcgcgcaac gccccgctct tcacatcctc
    21721 gggccagtcc gcggcgctcc ttcccgtggc cttctcaaaa cgggttcgga cggtggggcc
    21781 caaactcgac gcgtaatccg gatagcgcac gaaatccaga tgcacaccgt cggtcttgta
    21841 cgccgtggcc atctcccgca cagccttgag cagatactcg cgcactgcgg gcacggccgg
    21901 atcaagccac gggcgcggcg ttccgtcggg atgggtcagc agccaacccc gtttagcgaa
    21961 aattccgatc cggtcattcg tggccccttc ggttgagaag cagagcagcc aggcatgaac
    22021 ccgcaacccc agcggtttcg ccgctgccaa gcacgcggcc aactggtcgc cctgctcatc
    22081 gaacacacga gaacgcggca gcaccccgct ggcgtaatgc gcgaatcccg cacccgccac
    22141 attcacaaaa agatcggaga tccccgcatc tttgagcaga cggcaggtcc ggctccaatc
    22201 cccggggtag agtcccatcc ccgagtgttc ccacacgccg cggaactcgc ctttgcgaac
    22261 aggctgcgcc gcaccgtacg cctcatacat ccgggcccgc agatcctgcg ccgccagcca
    22321 cgcctcatac cctttgcctg cggccagcgc gcgcacggtc tccgcctcac tgcggagcgc
    22381 gccccgggcc gcgttcagag cccgcgtgcg acgggccggg tccgtgagcc gctcagcgtt
    22441 cattcgcagg ccttcggccc ccccggcccc gccgatcccc cgcgcccgat ccagcacacg
    22501 cgccgcagca ggccgccaga gcgacggatc ccgcgcagcc gccagagcca gaagcagacg
    22561 ccccttggcg tccgcatcgc catcggccag caacacatgg gtcatccagt atccgttcgg
    22621 cgacatcagc caagcggctt ccccggtctg ccgaccctga cggtcgtgcc accacgccaa
    22681 cacctgcgaa cggcctgcca caggctgaac ccgaaagagg tttctggagg tctggaggat
    22741 ccgctccggc gtgccgggcg gacgctgcgc agagaaacgc atcatggacc agcggccgtc
    22801 agttgaaccc ttctggtacc ccacggtctc catgcccaga agagccgcga gttccgctga
    22861 agaggaatag catacgatca tgctgccgcc gcgacctgca aaggccttca gcgcggcgag
    22921 ttgcatggca ttcggctgag aaagatagac cagcaccgca acgcgggcgg acgcgaggga
    22981 acgggacaga tcgcggtcat cggtcagcac cgcctccacg ccgacctcgc ggtaccaacg
    23041 ctccacatgc cgcgcaaggg atgccgcaaa acggcgttcc ccctcactga cggattggta
    23101 gccgtgaatc accgccaccg gtgcggcccc ggccgtgagg gcggccatca ccatccacat
    23161 cgtcagccgt cgtgtcatcc tcccccccaa gccctcatcc cacgccgtgc tcagcaccgt
    23221 tcctcaaaac gaatccgggc ggatcgcccg tgcgcatcca aaccctcgac ctccgcaaac
    23281 gtttcgatcg tgcggcgcgt ttcgatcaga tcctcccgcg tgaactcgac aaagctgtgc
    23341 cgccgccgga aatcatccgc cgtgaggccg ttgaacatgc gcgccgcgcc gcccgtgggc
    23401 agcacatgac tgggaccggc cacaaaatca cccgcagcct cgggagtcca cgggccgacg
    23461 aaaacagcac cggccgcgcg cacctgattc agccgcagcc gcgcgtcgcg cgtcatgatc
    23521 tcgaagtgtt ccggcgcaaa ccggttcacc aattccatac cctgatccag atccggcacc
    23581 actaccgcca gaataccgcc gacggcaagc acgcgctcga tacaagcggc acgggaaagg
    23641 agccgcgtct gcgccgtgag cgcgtcgcgc accgcccgag ccagaaccaa cgaatccgtc
    23701 accagcaacg ccttctcgtg cccgctgccg tgctcggcct gagacagcaa atccgcagca
    23761 acccattcgg gcgcggcgga gtcatccgcg aggatcgcga tttcactcgg tcccgccacc
    23821 tgatcaaggg ccacaatgcc gtacacctgg cgtttggccg cagtcacata cgcattgcca
    23881 gggccgacga tcttctgcac cggccggacc gtggccgtac cgaaggccat caggccgatc
    23941 gactggatcc cgcccacccg gtagacctcg gtggcgcccg cgacccggag agcgtgaagc
    24001 aacacggggt tgacccgttt gtcgcggccg cacggcgtac aggccacaat ctcccgcacg
    24061 ccagccgtgc gggccagcgt gacggtcatc accgccgtgg aggcgagcgg cgcagtcccg
    24121 ccgggcacat aaacaccgac acggcccatg ggcgaaaaga actcgccagt aaagccgcca
    24181 cgcggcaacg gcatgtgcca cgcctcgcgc agactggcct cggcataccg ccggacccgc
    24241 gcatgagcgt cgttcaccgc gcgccggacc cgtgcgggca cctcgcgggt tgcggccttg
    24301 agttccgcat ccgtcacgcg catctcggag acccggagcg cggcaccgtc aaaccgccgg
    24361 gccgcggcca gcacggcccg gttgccgtgc gcgcgcacct ccgccagcac tgcggctgcc
    24421 gcagtctccg cctctgggga aaaggccgga cgcgcgagga accgttcgac cttaggcgtc
    24481 ggacgggtta gggaccattg ggctatcgtc gcacgcatgc tcgggcctcc tcgctctccg
    24541 cctttcgtaa ccggcctcat tggtcgcccg caggcttctt ctcctctttc ttcgcgtctt
    24601 tcttcgcctc gtccttggcc ggctcgtcct tagccggctc gtccttagcc ggctcgacga
    24661 acgtcacgcc gaaagtgggc accgggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24781 nnnnnnngcc ggctcgtcct tagccggctc gacgaacgtc acgccgaaag tgggcaccgg
    24841 gcgtccgagt ttctcggcca tgcgcttgct gatcatcgca aaggcatcct cgaaggcctt
    24901 ctcgcggctg gcctctttgg cgaggtcctc atccttcatc atcttcttcc gcctagactc
    24961 tttgtccatc tcttcgagtt gcttctcgtt gatccgctcc tccagacgcg cctttctggt
    25021 ctcggccttt tcgcggttcg gaaaatctgc gcccatcttt ttggcggagc gcggcttgat
    25081 cttgtttccc gggaagggga gtttgttctc cttgttggca tcccactgct ttttgctctc
    25141 ggccgccgcg accgtatacg ccgccgtctc ctctctgatc tccttctgga gcgcggtgta
    25201 ctcttcgcgg ttcaggatct caaacccggt ctgaccgcgc atatccgtga tctgaaccag
    25261 actgaccatc tcggcgcgcg cgaggcccac cgccatcagt accattgcca tctgcatcaa
    25321 cccgttccgc atccgacacc tccttctttc tttgctgcgg gcgaaaggcc cgcacccgat
    25381 catttcgatt gctgtcccgg cacccactct tctgaaaaac ggggacggcg ctgcgccacc
    25441 gccagacgcg gggccgcagt cacagccgaa cgcgcgggcg tttccgcacg ccgcgcgggc
    25501 gcgggccgcg gcttctccgg cttccggggc tcaggcttgg gttcgggctt ggccgcagcc
    25561 ggttctttgg gctgcgccgc attggcggga accgcggcgg gcgacggctc cgaacggcgc
    25621 ggacgcgcat ccccgcgcac ggccgtgccc ccttcccccg cgcgctcgcg ttgggccccg
    25681 gcgggacgac gcgagttggc agactccgac ggcgacgcac gccgcgaccg gcccgccggt
    25741 ttaggcggca acttggccag cagcggatca tcgggcgcca agaccgtgca cttcagttcc
    25801 tcgccgatgt acttctcgat ctcggggata atgaacgact catcctcatc ggcgaacgag
    25861 atggccactc cgctagcccc cgcgcgaccg gtccggccga tgcggtgcac ataatcctcc
    25921 ggctcgtaag gaaagtcgaa gttgacaacg aacccgatgt cgtccacgtg caggccgcgt
    25981 cccgcgacat ccgtggccac cacgatccgc acccggccct cgcggaaatc ctccagcacg
    26041 cgcagacggc ggttctggtt gacgtcgccg ctgagcatct cgcaacggat gccgcggcgg
    26101 atgaggctgt ccgccacatc ctcggtcgtg ttgcggcggt tgcagaaaat cagcatacgg
    26161 gaatcggggt actgctggat gtggttgtag agcacggtga acttctcatt gaccgtcacc
    26221 acatacacca cctgcttgac cgcggcggtg gtgacctgct cggactccac ctccactttg
    26281 accggcgcct ccatccactg gagcgcgagg cgcatgacct cttccgtgag cgtggcactg
    26341 tacagcatgg tgctgcgccg attcttgggg ggcagccgcg cgataatcga acgcacgtca
    26401 gggatgaacc ccatgtcgag catccggtca gcctcatcga tcacaagggt gtccaccttc
    26461 gacagatcaa tcacacgcga ccgcacgaaa tcgagcagac ggcccggtgt ggccaccagc
    26521 aggtccaccg gcgcgtccga aacctcgcgt ttctgccgat cgtaatccat gccgccgtag
    26581 accgccagcg agcgcagccc gcaatactta cccaacgcct cagcgtcttt gcagatctgg
    26641 atcaccaact cgcgggtcgg cgcgatcacc agagcgcggg gcgcgccccc cttctgctgc
    26701 cgcgattcgg gagaacggag atagcgggtc aggatcgcaa ccagaaaggc tgcggttttg
    26761 ccggtgccgg tctgggcctt acccgccaca ttcttgccgg ccagcgcatg ctccaagctc
    26821 agcgcctgga tcggcgtgca gtactggaag ccgagatccg cgaccgcgtg caggatctcg
    26881 ccgggcagat cgaagtcatg aaaccgcttc ttcccttcgg ccggttccac cgaaaaactc
    26941 tccggcgacc acgaggcgtg ttcggcgatg cgcgccgccc gttcggaatc ccccaccgca
    27001 ccgcccggac gccgtgtgtt cggatccgag cgttcgccgc cgccgcggcc gcgcccgcga
    27061 cgcggctctt tggatggcgg acgggcaacg gaacgcgcgg ccgccgcgac cgcgggcggc
    27121 cccgattcgc ggcgcggccg cgtttcagaa ccgcgtccgg acgactcccg gcgcggtttc
    27181 ccgcgaccgg catccgcctt gcgtgcgccc tcgcggcccg aagcgccccg ggacgaccgg
    27241 ccagccgaac cctctttgga cgcctgaggt ttggcgcgcc cgccctgcgg acggctccgc
    27301 cgcccgttgc gcgacggttc ccgcttggga ttcttcgcgc cccggccagt cttggccgcc
    27361 ggttttttct gtgactgttg cttttgacca agtagtttgt cggctatttt tttaataaat
    27421 ccgagtgaca tatgcgtaaa cctgctatca gaaaaaaatg cggacgcctg cgagagacgc
    27481 ccgcacattc aagtaggatg tgacgaaaat aagactaacg cttcgagaac tggaagcgct
    27541 tgcgggcgcc aggctgaccc ggctttttgc gctccttcat gcgggcgtcg cgtgtcaggc
    27601 agcccgcctt cttgagcaca tcgcgcaatg tggcgtctga cgccaccaac gcacgggaga
    27661 gaccgtgacg gatcgcgccg gactggccgc tgatgcctcc gcccttggcc gaaacgaaca
    27721 cgtcatattt cccctgcatc ccggtcagct ccaacggctg actcagatat tggcgcaggg
    27781 cctcgctggg aatgtattgg tcgatcgcaa cgtcattcac gaaccatttg ccatcgccgg
    27841 gaaccagacg cacgcgcgcg atcgcggttt tccggcggcc tgttcccgct gcaatttctt
    27901 tcgccatctg aaaccttctt ctcttgtccg gccctgagcc gggatcctat aactctgtcc
    27961 gccgcctgat cagaacgtcg ccgcaaccgg catctgcgcc tcgtgcgggt gttccgcgcc
    28021 gcggtacacc ttgacgcggg tcatcatctt ccggctgagc gtgtttttcg gcagcatgcc
    28081 ccacaccgcc tctttgatca cgcggtcagg gtgtttttcc aacatggtct ttgccgtgaa
    28141 acgcttgagc cccccacgcc aacctgaata ccgctggtat tccttctgct cttccttccg
    28201 accggtcagc aggacttttt ccgcattgac cacaatcacg aaatcgcccg tgtccacact
    28261 gggatcaaag gtcggacgat tctttccgcg cagggtgttg gcgatattca cggctagccg
    28321 cccgagcggt ttacccgtgg cgtccaccag atgccaactg cggttcggac ctggatcttt
    28381 cggttttgtc gtcttcgtca tgttgaacct gtttttcctt cttcgcgaaa aatggtccat
    28441 aaggttatcc catcccgcgc ccggtgtcaa cgcgcaactt ccggttttta cacccgcaaa
    28501 accgaggccc ccagcgcggc cagcagggcc cccatcagcc cctgtagaaa cccggcggcc
    28561 cggaacaggc ccgggcgtcc ggtgagccag tcgatggctt ggcggagttt ccacgggtag
    28621 agcagcagca ccatgccctt gatgatgcac aggtaggcca cggccaccac caccagcctc
    28681 cagggcgagg ggtgggtgcg ggccgtgtgc agcagatcat agggaaagag cgtcagcagc
    28741 ccccccaacg cgcggcacgg caacaggtta tcaagccaga accaggtgag agggatgcaa
    28801 accagtaccg caaaggggat gtagcgcttg aacggcatga gcaggtcgag ccccatggtc
    28861 cagagcgcgg ctccggccca gatccacgcg agggtactca gcacgatccc ggccgcgcgg
    28921 ttgcgcggaa aagcccggaa cacccgtgcg gccggtccgg gacagaggac aatcgtcacg
    28981 ccgagcgaca ggcagaaaag gccgagcaga taacaccagt tcgaaaggga catataggaa
    29041 tctccgtaaa atgaggatag taaagcatat cccggtgcag gaggggaagc ttgatccgcg
    29101 ctcagtgacc tccatcgccc ggggcccgga ttcgcccccc cccgatctcg aagtcaaaca
    29161 taagcggcag atgatccgaa agtcgcaccc gggggatctt gaagcgcacg ggggtgatga
    29221 tggaagggtc atagcagatg aaatcgagtt cccggcaggg agcccagctt gggaaagtcg
    29281 gcgcgtggcg cgtgttcgcg ctccggagtc cggtcgcccg gaggaacatg cgcatctccc
    29341 aaacgccgga gagggtgttg aagtcgccgg ccaccagccg gggcttgtca caccccgtga
    29401 tcagatcgta caggtcgcta agctggcgct gccgcgcgcg tatacccagc gccagatgca
    29461 caagaaagaa ggtgacgttc tccaactcga cctcgatggc cagacttttg aaaccggttt
    29521 cgaaatcgtg aaaccgggtg cgcagaatcg gttcccgcac caacacggcg ttcgcctgtt
    29581 tgttgaacac cggcagacgc cgccccatgc cctcgtccgg atacttcacc cgacaacagt
    29641 ggtagagcga gagatccgcc gccagctcct cggcctgatt ccggtgccgc gcgcggtaag
    29701 atccggcgtc cacctccacc aaccccacaa tatccgggtt catatggcgc acgaaccgcg
    29761 tgatgttcgg aaagtgcgcg gtagtccgca acagggtctc cacccacgcg aaccgccgca
    29821 accggccagt cccatactga atgttgtgca acagcaaccg catacgtcct ctacttgacc
    29881 ggcagcgaag gcgccagaga cttcagatcg gtccaaagcg tgccctctcc ggcgtggagg
    29941 atccggatac ccgcatcggg gttgagcttg tccacaaagg tcagatcggc cagcgtggcg
    30001 ggatccttga aaacccccgc gcgcaactgc tcgcccagcg ccttctcgtt ggccgtcagg
    30061 tagtcggccc ggacccgggc ctcgccctgc acccgtgtga tctgcgcctg caccgcagcc
    30121 ttcagaaggt tggtggcggc aaccgcaagc gcggtgccgg cctggatgtt cgcgacctcg
    30181 ctgcgggtcc gcgccacggt cgtggccacg agtttctcgg tctcctgctc gacctgtttc
    30241 ttgaactgtt cgatcatcgc ggtttcggtg ttgagctccg cctgtttgcg ggccgtgtcc
    30301 tgacgcgcct tgttggtcag atcctgttct ttcgcgagac ttgaatcctg aatcggagta
    30361 aggatctcgc ccggcacctc cacatggcgg atgatcgcgt tatggataac aatgtgcttc
    30421 tcgcccatca cgcgcgtgag ttcagcggtc agttccttct ggaaaagctg gcggccctcg
    30481 ccgtcgatga aatcgcgcgc cttgtagctg gatccccgga tgcgcgagat ggagagcacc
    30541 tgcggcagga tgatcttctc gaccacggcg ggcagatccc cgtagagcat gtagatacgc
    30601 gagatgtcct ccgggagcag ctcgaactcg accgtcatat caagcaggat ctgaaaaccg
    30661 tcccgcgagg ggaactccac aaagcggttg atgccgaacg ccacggactc cggcaccaca
    30721 gcctgccgca ttgcggcggg tccatcggta tccgcaagca gatcctccgt ttccggtgcc
    30781 cgtcttcttc ctcccggcgc ctccatcgcg tacgctttcg actccctcgg tgacgcggcc
    30841 gacaccttgc gcgacaaggc cttacccaaa atctcccgac tcttgcccag cgcggcggca
    30901 acctcgtttt gggccagcag gttgctgttc cgcccaatat agtcggcgcg cttctgctgc
    30961 tgcttctgaa tcgtggtggc atgcagctca tccaatgcgc tgctggcatt ggagatttgg
    31021 ctcttggtca gcaccacgct gccggagcgg ccgttgatcg agacctggtt catgccgatc
    31081 tccagcacgt ccacctgata ggcgcgcgga ttgacatagt aaagccccgg ctggagcacg
    31141 tccttcataa cgccctgctg tccgggcccc gcgaactcac cggtcttcac cggctcgccg
    31201 ctctgcgacg tgacgatccc cacatacccg atcggaatgc tgaccgcgtc caactgggtg
    31261 atctcgtacc cttcgggatt cagacggtag gtgccgggac cgagcacgtc tttccacacg
    31321 cccttaccct ccttgtccgc agccaagatc tcgccgggcg gcaactcccg gccgactttc
    31381 gcggtcacca ccccgacctt gccgaccgga atggaaacca gcggcacgat gcgccacgag
    31441 tgggtgactg gatcgcggaa gtggcggcct tcgggcagcg ggtcgcgctg cacgcctttc
    31501 tcaccgggac cggcgaggat ctggccgggc ggcagcgggt gccccgtctt tctggtgatg
    31561 atcgccatat ggcccgcagg caccgggata cggcagaaga gccacagcca ggcggcagcg
    31621 aacagggcga gggccagcac aggaaagaga gcgagagaga ttttcttcat ggcgtgtccc
    31681 ctttcgcagg tttcaccgtt ccggccgcag gtgccgcggg accgcctttc gcccccaccg
    31741 gcaggccaaa caaaccctga ccgtcgtccg aggtcaacac cccctgaatc ttcggcgcgg
    31801 ttttctcata aagtctggcg cgcacgtaat cagcctcatc gcggtaaacc gcaacctgct
    31861 gcttgagcac atcggcgttg gcccggttct gcgcctcgat cacgcggcgg tcggcctcgg
    31921 caagttgaag caacgcctcg gcatccgcac gcgcgacttc gagctggatc tcggctacgg
    31981 acagctcggt acgggccgca agggtctgct cgatccgcac ctgttcggcc gcgatggtct
    32041 ggcggatgcg ttcggtctcg gccgtgactt tggcgctgtt ctggatggcc agcgcctttt
    32101 cgcgctccag atccgcttgc gaccgggcct gcacaatctg ctgctcgaac ttcagagact
    32161 cttggtttgc cagttcccga tcccggatga tgcccgcaac ctgctgcggc gcgatgatat
    32221 cgcggatcag caccgaattc agcgagacgc cccagttggc gcagatcccg cgcagatact
    32281 tctccaagct gtcttggaag gcctgccgcg actcgcccac aatgaactcc gtggcgctct
    32341 tcttgctccc ctcgatccgg aagaacccgc gcgcgctcgg caggatcagc ttctggcgaa
    32401 tgtcgtccat gtcgccgacc tcatgcgtca gcaacgcgac cttgtcgatg ttgagattga
    32461 actccagcgt gccctcgact gtcaccggaa aaccgtcgag cgtgaggaac atgatcgcct
    32521 cctccccgct gaactcgaac cgctggctct ggatgttgac gatcgaaacc gcgtacagga
    32581 aggggttgag gcggtgcgtg ccctccttga gcacctcgga ctgcacgccc ttgcatcccg
    32641 gcgcggccag aaaccccttc tcgcggacca cgtccgatcc gtctattccg ctcaggatgt
    32701 cccgaccgca tagcgcggtg accaccccga cgtgccccgg cgcgatcttg atgtcgtcga
    32761 acaaccggac ctcgtaggca taagggttga tccggtgctt gccggtgccg agcacgtcgc
    32821 gcacgatacc cttgtacccc tcgtccggcg cgaggatctc cccgcccggc aggtccttgc
    32881 cgtatttgcg caccagcacc ccgaacttgc cggccgggat gtccgtgacc cgaacgatct
    32941 gccaacccca cgtataggga ttgcggaaga aacggccctc gggcagcacc tcgagttgaa
    33001 tgcccttgta ccccggctca tccgcgatga tccggtccac cggcagattt ttaccggttt
    33061 tccggaacag caccgccatc ttgccattgc ccggctcgat gcgccaccag aaccagaacc
    33121 aaatcggccc gagaatgagc gccagcatca ggagcaacgg cgccagcgaa agggaggaaa
    33181 gcgcggcccg gcgcgacggc gtacgacgcg acggcgcgga tcgtcgattc tccagcggcg
    33241 gcggcatgat ttcggcatct tcctgcatgg tgacctccgt ttgttgtgaa cctgaagaac
    33301 ccgtcggcat actgaacgtg agagaccgga cttcagggcg aagagtctgt aaagctgtag
    33361 ggtttcagta taaaggggca aggcatggag cgtcaacgca aggatttagg gaaagacgca
    33421 tccgggcgca tccctgctgt tttgactgcg gtcaagcggc gggagtccgc aggagagaat
    33481 cgggggaaga accgcactgg acggctgaca acaaggacga ccccgatccc aatgacgata
    33541 gcgatttgaa cgtgtacggc ctccggacgg acggcttgtg tgcgagaaga gacggtctgg
    33601 aattgcactt gaaacatctg gacaggcgca cgaacctcta agtatattat ccacgcaaag
    33661 agcaagagga accacaaatg acgacatcat cggcgatcac cccccatgcc gtggcggcac
    33721 acctcggcaa gcccccggcg cagttcacca aggcggatat catccgcttc atacaggaca
    33781 ggggtatcca gatggtgaac ttcatgtatc cgggaggtga cggccggttg aagacgctca
    33841 acttcgtcat caacgatttg gcctacctcg acgccattct cacctgcggc gaacgtgtgg
    33901 acggatccag tcttttctcc ttcatcgaag ccagcgggag cgatctgtat gtcgtgcccc
    33961 gtttccggac agcctttctc gatccgttca ccgagatccc gacgctgacc ctgctctgct
    34021 ctttcttcaa caaggagggg cgaccgctag agaactcgcc tgactacacg ctgcacaagg
    34081 cttgcaaagc gttcaccgac atcaccggcc tggagttcca ggcgatgggc gagttggaat
    34141 actacgtggt ggcagacgac gttgcgctct ttcacgctgt cgacgaccag cgcggctacc
    34201 atgaatccgg gccgtacgcc aaattcaacg cctttcgctc ccactgtatg ctgacgatcg
    34261 cccagtgcgg cggcctcgtg aagtacggcc acagcgaggt gggcaatttc acccagaacg
    34321 gcaagatcta cgaacagaac gaaatcgagt ttctgcccac ctgcgccgag gatgccgcag
    34381 atcaactcgt ggtcgccaag tggatcatcc gcaacctcag ccgtcaacgc gggctcaatg
    34441 tcacattcgc gcccaagatc actgtgggca aggcaggctc gggactccac atccacatgc
    34501 gtatcatgaa agacgggcgc aaccggatgc tcgccgacgg cgcgctttcc gataccgccc
    34561 gcaaagtgat cgccggcctg atgacactgg cgccctccat caccgccttc ggcaacaaga
    34621 accccacctc ctacttccgc ttggtcccgc accaggaggc gcccacgagc gtctgctggg
    34681 gcgaccggaa ccgttccgtg cttgtccgcg tgccgctggg ctggactgcg gagaacgaca
    34741 tgagcgccgt ggcgaaccct ctcgaaaccg cctcggttcg ggacacctcg gacaaacaga
    34801 cggtcgagat gcggtcgccc gacggttcgg cggatgtcta ccagttactg gccagtctcg
    34861 ccgtagcggc ccgccatggc tttgaaatgc ccgacgctct ggacgtcgcc gcgaaaacct
    34921 acgtcgatgt gaatatccat aagacgggta acgagtcgat tgccgcatcc ctgagccagc
    34981 ttcccggctg ctgcgtcgat tcggccgcct cgcttgagcg tcaacgcgcc gtcttcgagg
    35041 ctcacgacgt cttcagcccg ggcatgatcg acggcgtggt ggcggccctc cgtgcctacg
    35101 acgacaaaaa cctccgtgcc gagctgatga agtcgcctga acggctcgcg gagcttgtcg
    35161 agcggcattt caactgcggt tagtcaaccg acgcgccccg tattttcggt gccgtttcat
    35221 ttgaggcaac ctgaagtcgc cctccccgaa cctttttgat tccggtgtca tgaatggtga
    35281 ggcaatgcga cggattttta cggattgacg gttttcaccc tcctgcgcat aatgaatacc
    35341 atcgtggatg tccgtgtgtt gtcgggcatc gtgtggctga tcgtggattg aacgtgatga
    35401 atgaagagaa cgagaaaccg gtgaaaacgc gggaggagct tgcggcgatt gcgtatctcg
    35461 acaagctgcg acgcggcgtg ggcgagacga tgccgacgga gctgccgctc gcgtgtgtcg
    35521 taaaggtggt cgcctacaag gagattccga aggcgaacga gaagtatgcg ctggtgaccg
    35581 ttcgcggtcc gatcgggatc acgtggaaga tgtgcgtcaa ccgccactat cttaaaaagg
    35641 gcatgaacgc gctcttcgtc tcaaaaggcg ctgcgatgcc cgaggaggag cgtttccgta
    35701 atcccgaagc cgcaaaggtg aagctgcgcg tgttcaagtt cggcccgggc atcaaggaac
    35761 gacggcttct gccgatcgtc agccggaaca tctatctctt caactgcggg ctcctctatc
    35821 cgctcgacga cttcccggaa ttacaggggg tacgcgcggg catggtctgt gccgtaaaac
    35881 tccgaatcga ccgcttgaaa gagctgcatg aacgtgtcgc cgcgccgaag ccaaagcaga
    35941 agaaaaagcc cgcgcccgcg ccgaaggcgg aatcggacgg cgatgatagc gtccttgcca
    36001 aagtgcgccg ctattacatc cgggtctgga gggcaagata aaaagcattg ccctgttttt
    36061 cacgcgaagc cgcgaagttt gtgtggcggc aagacggtgc cgcgcaacgt cagacaccag
    36121 gccctttttc ggcggtccgg gacgtcccgc cgtacctcca gcttgagcag atacgcgcgc
    36181 cagactttta catgacatgc tgttgcgagc gaggccgcat ccggcgcgta ggtcccgtcc
    36241 cgtatgtctc ggtctgtgcg gcgggcctgc ctcccgtcac cccgatcact catacgtcag
    36301 ccacgccgtc gccgtacagg cacggtcggc ggtcacgcgc aaccaatacg cgcgcgcatc
    36361 atccagcacc acacgggtgg tttggcccgg cgccacacga taggtatcgt acacccgcca
    36421 cacaccggta ccggtgaggt caagctccag cgtgaccgtt acgatcccgg ccgccgcatg
    36481 cgagagcgcc agccgcttac ggtcgaaccc ggtcatcaga taagggtcgg acggcacgcc
    36541 cgcagcggtg acgctgtcct tccaggggcc gccttcgccg cgcgctttcc cgaacgccca
    36601 gagatcgtcg acactgccga cccaaaccgc cgcacgtcca tctccggaac ggatgaggtg
    36661 acgcgacgta gccgcgttct cccggattcc ggtcatgacc agcaacccac ggtacgagca
    36721 gtaatcgtgc acctgcccgc aatgtgtcgc gacagcccgc accttgctga aaccgcccgc
    36781 attttcggcg ggcaactcgt agaagatgcc gcaaacgttc agcagatcac gttcggtgca
    36841 gacctcgcgg cacagacgcg tggcgccgaa aagcccaggc gcagcgtacg ccggatccgc
    36901 cttggggaaa cgccagcgcc gccccgcatc atcggtaatg agcgcggagg cggcctcgac
    36961 caccagcccc gtgccgctca tcggataatc ggtacgcgcc accttccacg ccgcggcatc
    37021 ctccttgcgc gtgaggttca gatcggcgtc caactcgtaa tatccggttt cacgcacacc
    37081 gtccggacca gcggtgaccg ccgcgagccc cagcacgcgc ttatccccgc cgcgtgcgta
    37141 cacgattccg ccgtacgcgc ggggatcccg caatgacgcc agacccgcgc aacgcgcgtc
    37201 cggccggtcg gaacggcgat ccgggttggc gtactggaaa caggcataga cgtccgcagc
    37261 gtcgcgctgc ggcacgagcc gcacccaggc accccgttcc cgttccgcga aggtcagggc
    37321 cgtcagccgc cccggtgccg cctcgaccgt tcgcacggct ttccagacgc ccttgcccgc
    37381 gcgatccgtt tcgagggtga agcgcaccgg ctccgcactg ccgtgggcga gcccgagcga
    37441 gcggaccgca tacccggaga acagatacgg atcgctcgga accccggcgg aaacggactc
    37501 tcccagccac acggcaccgc ggccgatggc cggaccaaac gcgtccaaac gctcaggatt
    37561 gacgaaccag agattcgagt gcgactgacc cggaccgcgg atcgtgcctt tggcacggcg
    37621 cttgttgaga aactccgatt tcgcgctgtc atcgcaagcc agcaccagcc aatcacccca
    37681 gcggcagaaa tccgccacaa ccttcagata gtttgaaagc ggcgcgatcc ccgccgaatc
    37741 gtgcgcggaa aaggtttttg ggaaacgcca gaaagtgccg tgcatggtca tcaggaacga
    37801 cttgccaccg atttcgcgga tgcgcggcca ctcggtgttc catccgtgtc cgccgtcgta
    37861 actgtggctg cctttgggca accggtaggt gtgccacgca ccgcagtcca gcacggtgag
    37921 caacagcgaa cggtgatccc aaccgatcgc ccagaccgga tccgtttccg ggttggggtt
    37981 acccaaaatg ccgccggggc cggtgacgtc ggtgaactga ttgcgccgca cgaccgtcca
    38041 cgccgcagcc ttgccgtccc aaacggcgag acatccggaa ggggccgagg gatcaacgaa
    38101 cgcctgccgc gcttggtcac cgttgttcgc atagatcagc aggccctgac ccgaataaag
    38161 ccctttaccg tggtaccccg gcaacttggc gaagcgggga ttgtcaccgg gagtgcgctt
    38221 cgccttcgcg agctgttcat cgcgccacag ctctgtcacg gccagtgtgc ggacatccac
    38281 ctcgtaaaag ccctcctcca tactcgcgta gtaaattttg ccctccggat cggtcaggtg
    38341 gcgcgcgttg ccggtgggcc gaccgtacat gacgctgtac gggatcgtcc ggaccgtgcg
    38401 atccgcgccg atcgcgtacg ggccaatgaa aagctgattg gattcgggat ggatcatccg
    38461 gttggcgggc gtgcccccga tactctcgga cctcacgacc gaaaccagat cgggcgtgat
    38521 ctcatagagc ttgtcatccg accctgtggg actatggggg gaataggtca gggtccacaa
    38581 ccgtccggcc cacggtacca ccgcaccgat cccgcactca cctccgccat tatacatggc
    38641 cagatgcggg tagatgccgc ttacaaaagc gtactgcccg gtgaccatcg gtcccccgga
    38701 cggcgcccgt acccctgcaa aaattgcgcc agccgctccc gcgaccgccc atacgaccgc
    38761 tgtcgaacac cacctcttcc aacacataac cgccaccttt ttcgttgttg atcagcgcac
    38821 cgcgcccgga aaacggcccg cctttcttgt catcgcccct ccccccttct cttgtcgtgt
    38881 ttcaaggaca ccggaccgga ccggttcccg cggtgccgac gtcaccgagt tcgaccgtta
    38941 cagtctctaa agaattcgcc ttgtggaaga cggctttcat cttacgcgtg gccggcatac
    39001 ccgaataggt gcccgtgcgc ggacccaccg tcagggcgcc gtccttgaag gcacatgtcg
    39061 tcagcgcgcc cttgcccgaa cggtattcga gggagacccc gtcatcttca tagagatcgc
    39121 ccttgccgtc ggcccccggc cacacatgca gctcgaccgt ctcgtcccaa cctttgtcca
    39181 catgcgcttt cttctcccac atcgggatca ccgcacccgc gcgcacaaac aggctgccgc
    39241 cccactccgg cgtcaccttg accgcgcgtt tgcaagggcc ggtcaccaac tcgcccgttt
    39301 tccagtcgtg ccacaaccct t
//
LOCUS       DAOSLE010000010        39082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1200_length_39082_cov_7.815564, whole genome shotgun sequence.
ACCESSION   DAOSLE010000010 DAOSLE010000000
VERSION     DAOSLE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 39082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39082
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_39082_cov_7.815564"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1557)
                     /locus_tag="PK576_01560"
     CDS             complement(226..1557)
                     /locus_tag="PK576_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE60308.1"
                     /translation="MNTTPIVENRFWQPEFECMPRPRILALQVERLRETVARAMRVPF
                     YRESFERKGITPDVIRSVEDVRRLPFTTKADFRDHYPLGLTAVPRSEIARFQGTSGTT
                     GKPTWTAYTAADVDRWADLCARFLVSGGLRPEHTVQISFGFGLFTGGFGLHNGIQRVG
                     AAVLPVSSGNAARQLMCMQDLQPEVLICTPSYALKLMDDILHGGVDRAALAIQHAHFG
                     GEPWTEEIRERIETGLGIRCWNNYGMAECGGPGLSGECAFRNGMHIQEDQFIVETIDP
                     ETLEPLPDGEKGELVVTPLCREALPVVRYRTRDIGILYADGPCPCGRTTRRMSRILGR
                     TDDMLIIRGVNVFPSQIETALMRFEKVAPHYMIEVTRPTALDEVTVHIEMQPGMFSDS
                     MREMQGIRERIAAEIYGVTGLHMRVNLVSPNTLERFEGKSRRVKDLRKLVD"
     gene            complement(1554..1991)
                     /locus_tag="PK576_01565"
     CDS             complement(1554..1991)
                     /locus_tag="PK576_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOE60309.1"
                     /translation="MKIQQISLFLENKPGHLNTICRTLADAGINIVTLSLADTQQFGI
                     VRLIVQEWQKAKEVLEQAGYVVNVREVVAAAVADRPGGMVEVLDIIGKTGVNIEYMYA
                     FAFRHNAEAVLVFRFDDPDRAVRALQAAGRDVLDAVTLFQEAK"
     gene            complement(1993..3354)
                     /locus_tag="PK576_01570"
     CDS             complement(1993..3354)
                     /locus_tag="PK576_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE60310.1"
                     /translation="MSLYEYAHDLDTPVHPVSAPDYTPVEELRALQLHRLQGLVRRVY
                     DHVPLTRARMDERGVRPEHIQTLKDIALLPFMMKTDLRDTYPFGLFACPMNDIVRFHC
                     SSGTTGKPIVIGNTQNDIRVWQNGCMRALALCGVRSSDIMQVAYGYGLFTGGLGLHYG
                     GEGLGCAVLPISGGNTDRQLMLMRDMGTTVIACTPSYFLHLIDEGMKKGIDFKRDMQI
                     RHGVFGAEPWTEEMRAKIESLTGIFAHDIYGLTEIAGPGVANDCRCRRGLHVFEDHFY
                     PEIVDPETLEPLPDGEQGELVFTTLTRTGTPMLRYRTRDISRLLTGACPCGRTIRAIE
                     RIHARSDDMLIIRGVNVFPSQIEAGLLSIDSVLPHYQIVVGKEGDLDTIEIKVEVTRE
                     TFSDDIKTLERLRQRIGQAVQRIINLSPHITLVEPHTLPRSEGKLQRVTDLRNRAVSS
                     KGV"
     gene            complement(3357..4208)
                     /gene="nifU"
                     /locus_tag="PK576_01575"
     CDS             complement(3357..4208)
                     /gene="nifU"
                     /locus_tag="PK576_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOE60311.1"
                     /translation="MWEYSDKVMDHFRNPRNVGKIENPDGVGTVGSLACGDALTLMFR
                     LDADGRIAEVKFQTFGCASAIASSSALTEMILGKTLEEAERITNREIAEYLGGLPDQK
                     MHCSVMGREALEAAIRNYRTGETTVKKRDDPIVCTCFGVSEGEIRRVVTENGLTQVEE
                     VTNFCKAGGGCGMCRDDIQKTIDVLRAQPVGPSVATVAAPAKKLTNLQKMKRIEEVVN
                     REIRPQLQKDGGDIELIDIVGDCVTIAFRGMCAGCHSAAFTRQDFIEARLREFVDPAI
                     TVIEEEK"
     gene            complement(4238..5347)
                     /locus_tag="PK576_01580"
     CDS             complement(4238..5347)
                     /locus_tag="PK576_01580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE60312.1"
                     /translation="MKSRRFWHTAGVLIGVSAAVWVCAAAELSGGREDPDGKTVWYDG
                     QSLPIEGRAFQDTEVFYDRLPARAQKTVTPGVWGNSRHSAGMAFRLVTDAPTVKVRWG
                     VRNASLAMPHMPATGVSGVDVYRRLPEGGWYFVRNGRPGSFTNSLTSFSITPNTEFMI
                     YLPLYNGTSFLEVGIPKGNRLFAPPVRASGITKPVVFYGTSITHGGCASRPGLAFTAV
                     AGRFADVPVVNLGFSGSGKMEMALCDLIAEIDASVYVLDCLWNMGDALVKERAEPFIR
                     ALRERRPDTPILLAEDCNVFGRAPTSKSLLMRAIYDKLKAEDPARWAKLHYLEAKDML
                     GHDGEGTVDGCHPNDLGMMRQGLVYGQALKKALGR"
     gene            complement(5434..6249)
                     /locus_tag="PK576_01585"
     CDS             complement(5434..6249)
                     /locus_tag="PK576_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE60313.1"
                     /translation="MMETIGQKLLDALKLYGVKVLFALGILLVGWFVARMIRQALKNL
                     MRKAHLDDTLVTFLASLSHTAVMILVIITALGQVGLQTTSFVAVLGAAGLAVGLALQG
                     SLSNFAYGILMVLFKPFRAGDYIEGAGVGGLVKAVGIFTTELRSPDNKTIIVPNAKMM
                     GDNIVNYSANATRRLELKICVSFADDLKKAKQVLESVLEAEPRVLKDPAPFVGVFALS
                     DYGVEFTVRPWVKTPEYWDVRFALLEAIKTRLDAEGITIPLPQQVVHLHRNEE"
     gene            6679..8118
                     /locus_tag="PK576_01590"
     CDS             6679..8118
                     /locus_tag="PK576_01590"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60314.1"
                     /translation="MIILGEKHTMSTLTRRTFLRAGGATTAAITLPNLIPPHLFGAAA
                     PSKQIHIGIIGCGRIATSMEMPGLFGLTEMARITALCDLDSKRLAHSKARVENEYNKR
                     LGLQNHTVKTFGRYSDLLRDGGVDAVMICVPDHWHALCAIECALAGKDIWLQKPFSQT
                     IHEGRILAEIVRRKKVVLQVGSQQRSSKQFRTACELVRNGRIGRIRRVEVGFGKDRDG
                     GRKEEMPIPPNLDYDTWLGPTPEAYYTEDRVHNQDIAQIASRPGWIQMEPYGWGMITN
                     WGAHHMDIVQWGLGTEETGPLKVSGTCQWLDKGLWNAHREFDLLYTYPDDIKVSVCYR
                     YPNGVRFIGDEGWIFVSRGAAKVTASDPTTPGKTLKALDASDPRLLEAPLHANAVRLH
                     VSDNHVKDWLLAIQNRTSPVTNAEIGHRSTSVCTLGHLCMKTGKTLAWDWRTERTDDA
                     GANALLACPERGPFSIPKVLQAADIRVSV"
     gene            8166..9557
                     /locus_tag="PK576_01595"
     CDS             8166..9557
                     /locus_tag="PK576_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013663935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOE60315.1"
                     /translation="MNRNTLLLTGAALLALTGCDNPGTRCCPNTCGGDTVKLITLDPG
                     HFHAALVQKRMFPGVSPKVHVYAPAGPDLSLHLDRIRGFNTRAEDPTSWESVVYTGPD
                     FFDRMIAERAGNVVVLAGNNARKTEYILKSVQAGFHVLADKPMAITPKDYELLKQAFE
                     VARSKGVLLYDIMTERFEITSVLQRELSRFPTVYGEQEKGTPDDPAVTKESVHHFCKQ
                     VAGKPLQRPPWYYDTTQQGEAIVDVNTHLTDLIQWETFPGVALTPADIKVLKARVWPT
                     PITLEQFKTSTSQTAWPDYLKKDLDAQGVLQCRANGEFTYVIRGVHAKASVLWNFQPP
                     AGAGDTHYSLMRGTRSSLIIQQGEAEGYKPVLYVEPRRVAGVNKEELGRALTDAVATL
                     AKTYPGISAEPHETGWRIIVPEQYAVGHEAHFGQVMEAFLGFLQNRDMPDWEVPNMLV
                     KYHTLMEAYRLSR"
     gene            complement(9684..12053)
                     /locus_tag="PK576_01600"
     CDS             complement(9684..12053)
                     /locus_tag="PK576_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HOE60316.1"
                     /translation="MSILMIATLSVGARTVESFNNGWEFAKDGGAFEYARVPHDWAIA
                     GPFDPEGDGDTAKLPWKGMGVYRKKLVMAEEPKGRVFLDFDGVMARSAVFVNGQVCGR
                     GDYGYLGYRADATCFLMKGTNDVEVRCSTKELTSRFYPGGGMYRNTWIVTTDNVYIED
                     DSLAVETVDVLTDLAKVKVSGKVTGRAATNRLVRVSFELTDPDGRTAATGKTEMELEA
                     FASARFERTLEVPHPQLWQMERGAKLYTLRIAIVADEYADELTRRIGLREFRFDPEEG
                     FFLNGERIQLKGFNQHADLGPLGMAVNEGAIRRQLDIMREMGANAIRTSHNPPAPELL
                     DACDEMGIFVWDECFDKWDDSAVKGDFVQEEFIARHLRQLVRRDRLHPCVFVWSMGNE
                     IQTARATKFGDVGWWIGPIALGTNRERCAQYRHAIREEDRTRPVGIGSAFGHALTGRG
                     DYLDLDIAGWNYGRVYAKAHELAPQIPVLCSESASAVSEFGFYADDLPTCKTNYAHEV
                     HRIDSYDFNAAPWSDVPDREFVRMELDPYCSGEFVWTGIDYLGEPTPFKDSRSSYFGI
                     CDLLGFPKDRYYLYRSYWNTEDFTLHIVPGHWNFPKKIGKKIPVFVYTSAPEAELFVN
                     GKSMGRRRKDPKAGTLDDYYSVLPRYRLMWMEVPYEPGEVKAVAYDAQGRKLGEQVRR
                     TAGVPVKVVLTPEKKRYGKLQVVMVTLADKDGNFVPDDDRRIHFQVEGCRILAVGNSD
                     PRGYDRFMKTDSHPLKFGRAGIFLEIEPGAKWKLTATAEGCVKAQKAAE"
     gene            complement(12084..14657)
                     /locus_tag="PK576_01605"
     CDS             complement(12084..14657)
                     /locus_tag="PK576_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60317.1"
                     /translation="MGGIVYDIDFGSATNQVARDGVGKINGVLPDDFAENYTGWCDGI
                     ATTEVKSEEGIGKYLRLTTSAQTKGGTQFVVPKELKPGCYRFTVESRTLGAVTYRCSF
                     RMGGSPYSDCLSRHVMSENWSVQSWCFSVPKDWKQLPMLFVNTRPGVVDLKRMKVETA
                     TIAEMAQEAVRRPSKDVTEYIRHNRFPLGLPTGWCFRRDCEGTVYGADETEPAPDGVP
                     VLKVSGNRMWTFYSEAFQTAFPGGEMEVSFKYRSDCRVRNFVNDDHWRGEMFGESWLG
                     PTKEWKEHRFRFRGPELAEAFTIGWGTGVEAGTMWLDDVRVRPMDGTGVRDTRPCLQM
                     AVVEGDAGEGTRVQFLDEKPLVKWCVVDVPTGSVMKVEAADLYGEEVTIAEIPLKGGK
                     LEKGTLEISAFKDCPMGQFRICATIEKDGKVVSEREEIVLTRVVRPVMWGKDAPNSPF
                     ASHFNPWRPVVKMMKACGVNWVRFHDAAEDLSNWFYLERKKGEWTFRDAAIDEYRRAN
                     VKIFAQLGMSPEWANHSADLVKENPKWTPGYFGRYLRPTNSVDWVNYVTTFEKHYRGV
                     IDEYFVWNEPGGHSWQFNDDAKWFAPGRAGQDFAVLQRQTYDALKAVDPKLKLFGFNA
                     GGPKEWVEDVMDGGGFHCCDGIDWHYYSVPSLAHRETANVMEKYSLPSIRAKYPDFGG
                     KPLYMSEGQGTNDGGSKLRCHNTGLYHATVPWTPDTMQDYLELSDNTARFLVQLLADG
                     FKKVFLYTCHGYVHPARRSEFSVLIGKDGYPVPSFVAHAALAQTIEGKTFVNKEDFGE
                     KGCVFTFRAKDGKNARVYSDLSEKEKLALAKKTCLHDVFGNPVTEKTAMPDTLVYEPG
                     K"
     gene            complement(14830..16944)
                     /locus_tag="PK576_01610"
     CDS             complement(14830..16944)
                     /locus_tag="PK576_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60318.1"
                     /translation="MGRTVRPARPWVRGTRVPAVVLLVLSFTFFIFNPSFAQGDEGHA
                     ATVTWRIHENQWRTEEEFKKLTKFFDEHPVTGKIAFFTCPSHVPPKFEHFLPCLPQLK
                     KAIAHFKARGHRTGINHLCTIGQTDEDLDRAIVIEGAQYLVNWKGETAKSARCPLDPV
                     WREKYVKPIYTVLAETGPDFIWTDDDIGRLSGHGVPGPGCFCPICMKRLHDRLGYSGD
                     VKGLNAFFTDAEKGEARRRAMMTLNREVYADLLKYIREVVHAVDPKIVIGVMDSITAY
                     TGNNYEEKFRALAPTGTEEVFFRPGGGYYNDIGGLGGVLSKANEMGYESAYLPDGVAV
                     NESEIETFQYQLLHKANFSLKTESLAYMAAGTRGTAWNVFPFADNETYEAVAPRIESC
                     EKVRVEADAIVAACGRAKPRGVWNGRGRDYFVGNKNGGRTGEWMQEEWWEKGFWQSEL
                     QMTGLPAAYREEECEVCAPNAQAVWSWTKEQLERRLAGGLYLPLDAAQAIIDRGYGED
                     LGFKIGKAVSKDARERFVDHPLNAGVAGFRRDMRQSFWGGDVIELEPMEGAQILADAV
                     DIPGRVVAKCVSGVYENRRGGRIFMTGTMAWDRLSFSPVVRERKNVMRWLSRETLTGY
                     IDSYHKAAMWVRGDRAIAIYNMSFDAAEGMEVLLKGEAWSKGIRLSGGDGTVIRGVRE
                     GAYTRYRLPKLAAWSVTAWARM"
     gene            complement(17038..19158)
                     /locus_tag="PK576_01615"
     CDS             complement(17038..19158)
                     /locus_tag="PK576_01615"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60319.1"
                     /translation="MKSSKIMNSCTFVVLVVSPFACSLSHLNAAEFRVLPGDDVKAVV
                     AQADAVRRQKPTEPIDIVFAKGDHEITVPLFLHRTKNPIGDGQAATMFRGEEGARVLG
                     GTRVGPWRKCAFNGRTDTWCADVPKDVERIYTFHADIEIRTECRYPNFDPEHPETGGW
                     LFIPGKMFSMYSLREGERKDRIPMRAEDWKTWAKPSEGRVNIFPRYNWWNRIPPIAAA
                     DEATHTLHLAGDTGEFPRPADRFYLIGLREELDAPNEWYFDADAHRIWFIPPAGMDPN
                     KVRTAITTTTQPVFNFNDVGNVHFRDLEICEGGGGIKLYDGSRNCTVTHCTIHDTGWF
                     AGCGIQTWGSGIVIRECRVWNTGNSGVQVHSLRDASPAAYAPGHVDGNEVSFCHIHHT
                     GRINGCGECICGAGQGTRIVNNLLHHAPRGAISFGGRMNDISRNRIHDTNFQSEDTGA
                     IYTGGYVNNAGTKINDNWISDSYGFSHGGTGVWEHRKADAFGIYLDDSSGGAEVVGNL
                     VERCTQGAIFFHSARFNTVSNNFFISNGGFGKYRLQITMNGFTTNAPPYLKTAAQDQY
                     DHMMKGGAAWTNYPSLRLPPTAFEHSEGMLMIGNQIVNNVVYYPDQPESFYTAPGQFA
                     PKATLFDRNVIWCGKGNRVRYGRNFKEIPAEKWAEMGQDLHSVVADPLFVDPARGDYS
                     FKPDSPALKLGIRPFKVSHYGPNY"
     gene            complement(19155..21467)
                     /locus_tag="PK576_01620"
     CDS             complement(19155..21467)
                     /locus_tag="PK576_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase TIM-barrel
                     domain-containing protein"
                     /protein_id="HOE60320.1"
                     /translation="MARVTPEWAGKVVFEDVARKAGEKEFCEVRSLPDGRIAVKGNTP
                     NAQCAGYGKYLRKVAKVPYFWEGGGTAAVAGRHAPPRMDGVIRAEAPYQWRAVFNFCT
                     HSYSLAFADKARWERELDLLALRGVNLPLACTGVECVWYETLMSLGWSDEEARSFLVG
                     PAFMAWQWMTNIESYAGPLPKSWIENRLALARFILARERELGMTPIQQGFTGNVPRDT
                     VKKFPSAKIKFQPPWCGFPGSAQLDPLDPLFPKFARAFYAAEKKLFGLHGYYAVDPFH
                     ESQPPQAGDDYLRAVGETIGRELRAADPAAKACMQSWSIRVPILKSFPKDGIIVLDIS
                     GEKGRHTKGFWGYPFVTGAIINFGGRTASGGDIRWLAENHFAETYRKFPNCVGMGYFP
                     EGIHTSPLYWEQALDLLWRDDNPEPLAWTTELLEARYGVPSATAKSLAEGLLATYYGR
                     VHLASAVYPAKPTFLLKRCDPNVGFRSGYDHRRLLRTWREMITAAKEFGVRPGMRFDL
                     VDAGHRLLSDLSYAQFVDVVEMFLKGDVKEYDCAVAAFLETGADEDWLRKHEPLSSLK
                     TYLDLAAAQGKTDEERNLYTFNQAVQVTYWGPFGVPTVAGDPIQEYAWKEWGGLLSGY
                     YLPRWKMFLEHARKVLEGKAELADIVPPTDIWQRPKHRGNAFYSKMADFEEGWIRDPK
                     RMTGDAPTDDVVSACEKVLAKQAAAFEYQFENGKEKINGFEAKIAEFEKEIQRRIKAG
                     RVRADVTGDGKENDTADLMRQVGDQMKEEK"
     gene            complement(21604..22878)
                     /locus_tag="PK576_01625"
     CDS             complement(21604..22878)
                     /locus_tag="PK576_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOE60321.1"
                     /translation="MERAKWKMAAAVAGRRVLPLLAFAAFTALAADPVVSGVTMTQDA
                     TTKNVTIGYTLSGADAIVTLDVTTNGVSIGGENLTKVWGDVNRKVVTGTRSIVWRPVM
                     GTADMKPVVTAWRTDTPPDYMVIRMDRPQGATNVVPLYYANTNSIPGGFNNARYKTTH
                     MIFRKCPAAEVVWRMGTPSSDGFFGADQVAHLVKLTHDFYIGVYPCTQGQFTALAGVG
                     WRGRCRYTYSANIDWAVHPMESINYDDLRGKTAGAQWPTSGDVDAGSFFGVLRAYTGM
                     NFDLPTEAQWEYACRAGTGTTGNIGVSYGTCWDEVSWINYNADGHTHEVGLKRPNAWN
                     IYDMHGQVMEICRDWYGPYEFVDDETPVVDPEGAPSGEYRVARPAAFTNNHTPNHSGF
                     RNKMKPSEGDMGGWHWNGSTYFAGFRVALEIE"
     gene            complement(22898..23170)
                     /locus_tag="PK576_01630"
     CDS             complement(22898..23170)
                     /locus_tag="PK576_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60322.1"
                     /translation="MENVKEKMVAVAVFMAMAAVAEPAWTFTIEDHPAEVTASATTTD
                     AVAVASVTSQEAPSGAGGLTAFVSAFFESLGINLRSDPFGTLFLFK"
     gene            complement(23183..24463)
                     /locus_tag="PK576_01635"
     CDS             complement(23183..24463)
                     /locus_tag="PK576_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOE60323.1"
                     /translation="MKRVVGMTAVFSLALAAGGAEPSILTNSVTMAQSSQTHLVTIGY
                     ELTGADAIVTLDITTNGVSIGAANIRGLVGDVNKRVSPGQRAIQWNPNKSWKNNVVSD
                     GSAKAVVKIWSLSEPPLYMTASLDTANCVRYYDCADAIEGGITNDMYKRTHLLLRKVP
                     ATGVTWRMGENSRNSGEYTGANETPHLVAFTNDYYISVYQFTQGQIAELRGLGWVGGC
                     NYNKASHDDWWSHPMESINYDYLRGATKGAQWPTTDEFDADSCFAVLQNRTGMRFDLP
                     TDAQWEFAARAGNQLASSTVAVDTYTSGWEIMNPYAWTGWNSGDNGGSENKRTHAVGT
                     KLPNNWGIYDMQGNVWEMCRDWFEQVCAFPADGSPVVNPLGPISGSTRVMRPGGYDNS
                     THSVHRYTFRNHIDPSGSASGSVGFRVVCEITGE"
     gene            24670..25926
                     /locus_tag="PK576_01640"
     CDS             24670..25926
                     /locus_tag="PK576_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOE60324.1"
                     /translation="MVLTTEIPRVVICFSAAIKTMRELMQGVLEYAKANGPWALHILA
                     GRNLEQKLPDLVEWGCNGLIGNLSDEQFVDMKIPADIPLILNGRASNEDDRPVDYPSN
                     TIGLIECDNVPIGRAAAEFFLSKGFTAFAYVGDVNRPQWSIRRGEVFARCVAARGFEC
                     HHYTLPPISVARNAVREQRTLARWLDALPKPIALFAANDTRARQVVNLCLENGIAVPQ
                     EISILSCDNDELLCESCVPSLSSIKMNPFQAGYEAASLLDRAMHEGFRRRHRPIRVTY
                     TLDSIVSRLSTADLRVKDPLVERALTCIRLNSAADFSVGDLAEKLKVSRRLLEMRFRK
                     VLDSTVHNAIMSARLERVCEVLKSTRLPIQDIADRLHFSSASHLGIIFKRQFGTTPSR
                     FRRELSGGGRNDFRLMAGDSTTVSRI"
     gene            26099..26419
                     /locus_tag="PK576_01645"
     CDS             26099..26419
                     /locus_tag="PK576_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HOE60325.1"
                     /translation="MKKSLVLSLLCASVLMTTGCLSPMKGSIMAVFTVDHVASDPVVD
                     NTVRPAKRGQAKATGIICFATGDASIGAAMRNGGITKIHHVDYDVKNILFLYNEVTTI
                     VYGE"
     gene            complement(26489..27379)
                     /locus_tag="PK576_01650"
     CDS             complement(26489..27379)
                     /locus_tag="PK576_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOE60326.1"
                     /translation="MAIRSMTGFGRGEAEENGRGVTVELSAVNRKQFDCSLSLPRELS
                     SLDTRIQTLIRTRVSRGYVKGVVTVATSVGEAGGNAAGWDPEVIGKHLTALRDLAKRL
                     GLADDLTASSLLCLPDLLRGAAPLEPEKMWPLVERAGTLALESLDTMRQREGAALEQD
                     LRKRLTVLEKTAEQIAARAPMVPVTYQTLLTKRLGELLGEEGTAVDAALVAREVAVFA
                     DRCDVSEELTRLGSHFAQVAKTLDSGGTCGRTLDFLCQELFREINTTGSKANDAEISR
                     HVIAFKAALEAMREQVQNIE"
     gene            complement(27421..28167)
                     /locus_tag="PK576_01655"
     CDS             complement(27421..28167)
                     /locus_tag="PK576_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60327.1"
                     /translation="MSDDLTDRQVLTRLAWLFALFAVISLAAGASRMQPPGWQLRTFG
                     DVLRLREALSMFVFAPALGLVFWLIGRTLSRGRTVVAADLLMVLAVYGVACGMGIHDP
                     TNRMISAYSGSGALTPEVRRSLFYLDEVLGHWVFWAGFVLATWVLGLRQALAPLKERM
                     QWPWRVGLGVVAAAFLWVMVTNLWDEYPQTRADLGVILLAALGPLAAHVAFGRNAGVL
                     RMPLHFVIYPTYFGSVAVTLINWLIRYGRV"
     gene            complement(28157..29788)
                     /locus_tag="PK576_01660"
     CDS             complement(28157..29788)
                     /locus_tag="PK576_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOE60328.1"
                     /translation="MRFLTVDQMRAADRDAVTEAGIPERALMRRAGEALACAVARVAA
                     RRRTRAAVLVAGHGNNGGDACVAARALREAGFLVRVLLTAPVARFRGVAREAWEAMDA
                     AGVPWSEPEAAESACGGWTEDNADAVAAPSAWGGVVVDGVLGTGCAGAPSGAAARAIG
                     WINRQRAHAMIVAADIPSGVHGDSGEAAGVAVHADVTVTFARPKRGLLNPCGAERFGH
                     VIVADIGLPDALCDRGTDDAPCECIALPELARFFPRRQWSAHKGTCGHVAVIGGAAGM
                     PGAPVLAALGAVRSGAGLVSLAVPPQSAAVAATWVPEAMCHTLDVPRGVISPHALAAG
                     FSGLDACKVVIAGPGLTRHEEAGRTVAQLLETGPDRLLLDADGLNALADLFHAGWRPR
                     ARQDLLLTPHPGEAARLLGLSVAAVEADRVAAVRLLAERYRAVVVLKGAGTLVCAPDG
                     RPWLNRTGNPGMASGGMGDLLAGMVAALWAQGLDPVQAACTAVWAHGAAGDEAAWEEG
                     RTALSATRLAAALGSVYGELERLAAGSEEGGGNVG"
     gene            complement(29776..31023)
                     /locus_tag="PK576_01665"
     CDS             complement(29776..31023)
                     /locus_tag="PK576_01665"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase family protein"
                     /protein_id="HOE60329.1"
                     /translation="MIPHELVRALPKSDVHAHLDGSLRIATLIELAREQRVPLPSYEE
                     EGLLELVFKPVYRDLADYLRGFEYLCAVLRRRDALERAAYEMAEDAIAQGVRYLEVRF
                     APQRLVETGEACVQAFKAVNDGLARAAREHNASARVHAQEDIPFEWAILCCAMRNFER
                     GMGAYYDRLLDVLPGMARNELVSIASLEAVRAAVKARDREGVPVTGFDLAGEEAGYRA
                     GHHVTAYEEAHRHFIRKTVHAGEAYGPESIYEAIARCHAERIGHGTFLFAADKILDPS
                     ISDPQAFTDSLADYIATMRVTIEVCPTSNLQTIPELGGDLRKHPVRRMLACGMSVAVG
                     TDNTLVSRTNINTELLRVAEVCELDRRAFQRLVLAGFKAAFYPGRYEVKRRFVRRAAE
                     RIEQLLGTVPKHAAGKKEAACGS"
     gene            complement(31023..32171)
                     /locus_tag="PK576_01670"
     CDS             complement(31023..32171)
                     /locus_tag="PK576_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HOE60330.1"
                     /translation="MRNEWYYAVEGERFGPVTFTELRRLAQTGRISEYDLVWHPEFGP
                     EWRNAGQVREMFEVPYREAAAPPISGMGEVTLGRPHALRAASQAFARMTALLFRPFDP
                     VRWFSIGFCAWLAYLGTQSTVNLQQLGETSAGGFKQQVDRMIDVLQLLPEQPVKLAWT
                     GFAILLGLLFALWMCWVRSRGDFMFLHRWYKPDTPVHACWMASRSSGWALFGWRVLFF
                     LVAAALFVLHAAIAYTHVLKPYLDAGRIWQPALLPPVTGCVTSLALLLIAVRLVAYLT
                     QAFVVPLMYWSGCGVASAWRMVLALVNRHPFALTGYLLCGMACWSAAMFAVLVFGLVT
                     CCIGFIPLVLPYLNAVLLLPVTFFFRGYAVCFLSQWRPDLVPAAEGDM"
     gene            complement(32190..33134)
                     /locus_tag="PK576_01675"
     CDS             complement(32190..33134)
                     /locus_tag="PK576_01675"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOE60331.1"
                     /translation="MTGEVGAGMRDLDLRVNLGGIRMQNPVTVASGTFGYGREYAGLI
                     DVTRLGAITVKGIRLQPWPGNALPRHVEVPGGLVNAIGLQGPGVDGFVSEYMPFLRTT
                     GVPVIVNIWGTSVEEYAEVARRLTEVEGVAGLELNISCPNVKEGGNTFGTDPCAVSSL
                     VSAVRRATRLPLIPKLAPNVPDIRVFVRAAQEAGADALAMINTLPAMVIDIETRKPVL
                     ANRVGGLSGPAIHPVAVKLVWEAAQVAAVPIVAMGGIMGAEQAIEFLIAGASAVAVGT
                     ANFIDPSTPLRVLDGLESYLRRHGMRSVGELTGSLCCG"
     gene            complement(33131..33958)
                     /locus_tag="PK576_01680"
     CDS             complement(33131..33958)
                     /locus_tag="PK576_01680"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOE60332.1"
                     /translation="MSASGLGGCGKGEAGMVDVWGRVRRQIPLTAGAWVMEMEAPEVA
                     AEARPGQFVHVRVPNLERSALRRPFSLYGAEGGVLRLLYKTVGRGTEQLSRVEPGERM
                     QVIGPLGNGFPLEPQGTPVLVAGGYGVAPLAFLASRLPVRGVVLVGGRGAADVLAIET
                     FTELGWRVRVATQDGSLGEQGLVTDLLDSELETMRGAEARVELFACGPDGMLRAVGDR
                     AIQTGCRAWLSLDKRMVCGVGACLACVQSLRRPDGSEWIGRVCHDGPIFEAREIIWS"
     gene            complement(33955..36921)
                     /locus_tag="PK576_01685"
     CDS             complement(33955..36921)
                     /locus_tag="PK576_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE60333.1"
                     /translation="MERFVQPENTARIQVPPTERLAAPAQDETQTVRVVPGEGTSHYE
                     EPGNDKLFDQYTIYSKIGNGGMGVVYLARDRRLGRFVAIKRLNHQAQSIPTLRQRFLH
                     EARAVAVLSHVHIVHIYALGEDEDGPFIVMEYIAGPDRPDVVSEMPPGGLLQPNPPLT
                     LDQYVSQHGQLSVDEAIELVLKIGRAVAYAHASGVIHRDLKPSNILMDKSGEPKIVDF
                     GLARLMSKEEAKLTVPGEKLLSLGYGAPEQESDASQSDERADVYGLGALLYFVLTGQN
                     PRYFREQDIPVSLRDVVVKALATDKEQRWESAAAFNTALYQIQSKTQIETPTVKTTWR
                     CKWCDAVNPLAIKFCAECGWDGSEACPECGADTFVGVQYCGTCGADARAYESVLHLLQ
                     KMRDSDTQQRFDRVIAHAGRVHGFEPAGPSGRAYLQEVTELRALAEKKMRRRDQLKEQ
                     IPIEIRAENFERAEEFIRQYRELTEDRQGFENELRQIPERILLRDLQRAQRALKQREW
                     DSAARLCGTLLREVAPDHPDVLRIRKAIRIHTAAANLRIACAITLGMMVLYLLSLPVA
                     VTLADRSFGTLARGFYRPARWVYERSLARVPLQAYARLWLGAPPEWNVYFATASEEPV
                     LPGEVQPTPQPDALAQKQREYARQLAELDARQEKFQRAWPTEYVRELDLLMERCRTAG
                     DFEGWDVAQTERRRFDETRQMNDAVVEGLSELNTLKTKYRQMLSEQRLQHSRKWVTLC
                     KKYVNDLTDMQRKFMQEGRMDLASAVNAEIRKARNAPPALAAEAVVAAAELAGMSETE
                     TAPMLLGGSDQHRVEEIAQLRSGFESELAAVEKDAVRKATAWPDQYVQGLTELMESFQ
                     RAGDYVGWESARDELGRFEADRAIQQRHFVLQPAGLVELQKKFFLLREENRRKRAEQL
                     VDTTEKYVKKLQDVQKKLTVGGQMETAAAVNAEIKRVRTRVDYIEAQNELSPPQGPPV
                     PPAVWPKTEPAAGGNG"
     gene            complement(37006..38928)
                     /locus_tag="PK576_01690"
     CDS             complement(37006..38928)
                     /locus_tag="PK576_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60334.1"
                     /translation="MVSLYTVGIAGGVLGILLGFALPVCGSDLALRFRTPRENWTRPG
                     VELTFRTAHTNQAEVVRQLERAQALGFGSVLAAVEGPEEGVWPIVERLADTCHRLGLE
                     LGLHDFPFAGDGGKNRARCLVWTEEPSTAFGGTNALNRVVQENGALAVWAVPADRSGA
                     VHPHEMVDLTRAALPTTGVWRVFRFALREVTPPLTDGYAGNSFARHVNRTLSACQSRL
                     PQTYGTTLLWYQFNGPQRDDMVWPADLPERFLRNSGLNLMRFLPVLAGVPVGGEATAQ
                     YVRRQLRQSVREAWREGFARHADELVHEAGLEAGIAIDAVPVEPEEVPLSFRRPMFAV
                     SRDADAAGVNRRAAGVARVLRRRFVIGRLPLNDLAETPAATLLPFRWKPEAERLLVEG
                     ATRLLLVPSGDLPDEDAAFRSLREGCDTVRRCQVLLQHGEPVADFLVWTEGVPPVLEG
                     YACDYATPALMERATVSQGRIRFESDRTYNALVVPAGVMGREAARRRIGQIAARGVTV
                     WLMEEADGAAGTTAAETMPAGCRRLRPGAPDAPRPDLFWQSEQAGMRLSFLHRRTPER
                     EIYFLVNDTPEAGPVRCTFRDAGAGMPLRWDPVSGETDLVVQGASRTGDGRVTAPLFL
                     GPHDACFIVFERAHAN"
BASE COUNT         7481 a        13703 c        10608 g         7290 t
ORIGIN      
        1 tctttcctaa acacgcttgc aaatcaatag agaccgaacg tcaggacaat gcaatcggag
       61 ggcttgcgga tcggtatcgg tatcgcctat cgggatcggt gcctcgttct tgcgtgaaca
      121 tgaaacgctg cgcggtgtac ggcttttgcc gcgcatagcg tgacccggac aagtgacact
      181 tgtccgggcc ggcgctccgc aagccgcctg tttacgcgtg ttggatcagt cgaccaactt
      241 gcgcagatcc ttgacgcggc gcgatttgcc ctcgaaacgc tccagcgtat tggggctcac
      301 gaggttgaca cgcatgtgta acccggtgac gccgtagatc tccgcagcga tacgctcccg
      361 gatcccctgc atctcccgca tgctgtcgga aaacatgccc ggctgcatct cgatgtggac
      421 cgtgacctcg tccagcgccg tgggccgggt gacctcgatc atgtagtgtg gagccacctt
      481 ctcaaaacgc atcagggcgg tctcgatctg cgacgggaag acgttcacgc cgcggatgat
      541 cagcatgtca tcggtgcgac cgagaatccg gctcatgcgg cgggtggtgc ggccacaggg
      601 acaggggccg tccgcgtaca agatgccgat atcgcgtgta cgataacgga ccacagggag
      661 ggcctcgcgg cacaacgggg tgacgaccag ttcgcccttc tcaccgtccg gcagcggttc
      721 cagcgtctcg ggatcgatgg tctcgacgat gaactggtcc tcttggatgt gcattccgtt
      781 gcggaaggcg cactctcccg agaggccggg tccgccgcac tcggccatgc cgtagttatt
      841 ccaacagcgg ataccgagtc cggtttcgat gcgttctcgt atctcctccg tccagggttc
      901 gccgccgaaa tgcgcgtgct ggatggcgag tgcggcacgg tccaccccac cgtgcaggat
      961 atcatccatg agcttgaggg cataagaggg cgtgcagatc agcacctccg gctgcaaatc
     1021 ctgcatgcac atcagctggc gcgcggcgtt gccgctggag accggcagga ctgcggcgcc
     1081 gacacgctgg atcccgttgt gcagcccgaa gccgccggtg aagagtccga aaccgaacga
     1141 gatctgcacc gtgtgctccg gacgcaggcc tccggagacc aggaaccggg cgcacagatc
     1201 ggcccaacga tccacgtccg ccgccgtgta agcggtccag gtcggcttcc cggtggtgcc
     1261 gctggttccc tgaaaccgcg cgatctcgga gcgcggcaca gccgtgagtc cgagcgggta
     1321 atggtcacgg aaatcagctt tggtggtgaa cggcagacgg cgcacatcct ccacgctccg
     1381 gatgacatcc ggcgtgatgc ccttgcgctc gaagctctcg cggtagaacg gcacccgcat
     1441 cgcgcgcgcc actgtctcgc gcaaccgctc gacctgcaag gcgaggatac ggggccgcgg
     1501 catacattca aactccggct gccagaaccg gttctcgaca atcggtgtag tattcatttc
     1561 gcctcttgaa atagggtgac ggcatccaac acatcccggc ctgcggcctg cagtgcccgg
     1621 acagcgcggt cgggatcgtc aaaacggaag accagcactg cttcagcatt gtgccgaaag
     1681 gcgaaggcgt acatgtactc gatgttcaca ccggtcttgc cgatgatgtc gagcacttcc
     1741 accataccgc ccggccggtc ggcgaccgca gcggccacaa cctcgcgcac attcaccaca
     1801 tacccggcct gttcgagcac ctccttggcc ttctgccact cctgcacgat cagccggacg
     1861 atgccgaact gctgggtgtc ggccagcgag agggtcacga tattgatgcc cgcatcagcc
     1921 agcgtgcggc agatcgtatt caggtgcccg ggcttatttt ccagaaacag ggaaatctgt
     1981 tggattttca tactatactc ctttcgacga taccgcgcgg tttcgcagat ccgtgacacg
     2041 ctggagcttg ccttcgctgc gcggaagcgt atggggctcc accagtgtga tatggggact
     2101 caggttgatg atgcgctgga ccgcttggcc gatccgctga cgcaaccgct ccagcgtctt
     2161 gatgtcatcg ctgaaggtct cccgcgtgac ctccaccttg atctcgatcg tgtcgagatc
     2221 gccctctttg cccaccacga tctggtagtg gggcagaacc gagtcgatgg acaggagtcc
     2281 ggcctcgatc tgcgagggaa agacgttcac gccgcggatg atcagcatgt catcgctgcg
     2341 ggcgtggatc cgctcgatcg cacggatcgt gcggccgcaa gggcaggccc cggtgaggag
     2401 cctcgagatg tcgcgcgtgc ggtaacgcag catcggcgtt ccggtgcgtg tgagcgtggt
     2461 gaaaaccaac tcgccctgtt cgccgtcggg caggggttcg agggtttccg gatccacaat
     2521 ctcgggatag aagtgatcct caaagacgtg caggccgcgt cggcagcggc aatcgttggc
     2581 gacacccggt ccggcgattt cggtcaggcc gtagatgtcg tgcgcgaaga tcccggtgag
     2641 gctttcgatt ttagctcgca tctcctcggt ccacggctcc gcgccgaaca cgccgtgccg
     2701 gatttgcatg tcccgtttga aatcgattcc ttttttcatg ccctcatcga tcaggtgcag
     2761 aaaataactc ggggtgcagg cgatgaccgt cgtccccatg tcgcgcatga gcatgagctg
     2821 gcggtcggtg ttgccgccgg agatcggcaa cacggcacaa ccaagcccct cgccgccgta
     2881 gtgcagaccg agcccgccag tgaagaggcc atagccgtag gcgacctgca tgatgtccga
     2941 actgcgcaca ccgcacaggg ccagcgcccg catgcagcca ttctgccaga cccggatatc
     3001 attctgcgta tttccgatta cgatgggttt accggtcgtt ccgctggaac agtggaaacg
     3061 cacgatgtcg ttcatcggac aagcgaagag gccgaaggga taggtgtcgc gcagatcagt
     3121 cttcatcatg aagggcagca acgcgatatc cttgagggtc tggatgtgtt ccggccggac
     3181 ccctcgctcg tccatgcgcg cgcgggtcag gggcacatgg tcgtagaccc ggcggaccag
     3241 gccttgcagc cggtgaagct gcagcgcgcg caactcttcg acaggcgtgt aatccggagc
     3301 actgaccgga tggaccgggg tatccagatc gtgagcatac tcataaagag acatgattat
     3361 ttttcctctt cgattacggt aatggcagga tcaacgaact cgcggagccg cgcctcgatg
     3421 aaatcctgac gggtgaacgc ggcagagtgg caccccgcgc acataccgcg gaacgcgatg
     3481 gtcacacagt cgcccacgat atcgatcaac tcgatgtcgc cgccgtcctt ctgcagttgg
     3541 ggccggatct cgcggttgac cacctcttcg atccgcttca tcttctggag attggtgagt
     3601 ttctttgcgg gcgcggccac ggtggctacg gacgggccca ccggctgcgc gcgcagcaca
     3661 tcgatggttt tttggatgtc atcgcggcac atgccgcatc cgccgcccgc cttgcagaag
     3721 ttggtcacct cctccacctg cgtcaacccg ttttccgtga ccacacgccg gatctcgccc
     3781 tccgagacac cgaaacaggt gcacacgatg ggatcatcgc gttttttaac ggtggtctct
     3841 ccggtgcggt aattgcggat ggccgcttcc agggcctcgc ggcccatgac cgaacagtgc
     3901 atcttctggt cgggcaaccc gccgaggtat tccgcgatct cccgattcgt gatccgctcc
     3961 gcttcctcca aggttttgcc gagtatcatc tccgtcagtg ccgaagagga ggcgatagcg
     4021 ctggcgcagc cgaacgtctg gaacttcact tccgcgatac gcccgtccgc gtcaagccgg
     4081 aacatcagcg tcagcgcgtc accacaggct agcgaaccga ccgtgccgac accgtcaggg
     4141 ttttcgatct tcccgacgtt gcgcggattg cggaaatgat ccatcacctt gtcactatat
     4201 tcccacatga ctccgctccc gcatcacgct cattccctta gcggccgagc gcttttttca
     4261 gcgcctgacc gtagaccaaa ccctgacgca tcatgccaag gtcgttgggg tggcatccgt
     4321 ccaccgtacc ctcgccatca tgccccagca tgtcctttgc ctccaaatag tggagcttag
     4381 cccatcgggc gggatcctcc gctttcaact tgtcgtagat cgcccgcatc aggagggatt
     4441 tggaggttgg ggcgcggcca aagacgttgc aatcctcggc cagcagaatc ggtgtatcgg
     4501 gacgccgttc gcgcagagca cggatgaagg gttccgcccg ctccttcacc agagcgtcac
     4561 ccatgttcca aaggcaatcc agcacataca ccgaggcatc gatctccgcg atcagatcgc
     4621 agagcgccat ctccatctta ccgctgccgg aaaaaccgag gttgacgacc ggcacgtccg
     4681 cgaagcgtcc ggcaacggcc gtaaacgcca gaccgggtcg cgaggcacag ccgccgtgcg
     4741 tgatcgaggt gccgtaaaag accaccggct tggtgatccc gctggcgcgt acgggcggcg
     4801 caaaaaggcg gttacccttg gggatcccca cctcgagaaa cgacgtgccg ttatagagcg
     4861 gcagatagat cataaactcc gtgttggggg tgatgctgaa cgacgtgaga gagttggtga
     4921 acgacccggg gcgtccgttg cgcacgaaat accagccacc ctccggcaaa cgacggtaga
     4981 catccacacc gctgaccccc gtggcgggca tgtgcggcat ggccagcgag gcattgcgca
     5041 ccccccagcg caccttgacg gttggcgcat cggtaacgag gcggaacgcc atgccggccg
     5101 agtggcggct gttcccccag acacccggcg tcacggtctt ctgggcgcgc gcgggcaacc
     5161 ggtcgtaaaa cacctcggtg tcctgaaagg cgcgcccctc gatcggcagg gactggccgt
     5221 cgtaccagac ggtcttgccg tccggatcct cgcgcccccc cgaaagttca gccgcagcgc
     5281 acacccacac cgcagccgac acaccaatca gaacccccgc cgtatgccag aaacgcctgc
     5341 ttttcatgac cgctgtcccc tctcccgcgc atggcctttt tcttccggat gcccgggacg
     5401 gcgcaggggc gccccgcggc cccgtctccc ctcttattcc tcattccgat gcaaatgcac
     5461 aacctgttgc ggcaagggaa tggtgatccc ctccgcatcc aaacgcgtct tgatcgcttc
     5521 gagcagcgca aaccgcacat cccagtattc cggggttttc acccaagggc ggaccgtgaa
     5581 ttcgacaccg tagtcactca acgcaaacac gccgacaaac ggggccggat ccttgagcac
     5641 gcgcggctct gcctccagaa ccgactccag cacctgtttg gcttttttga ggtcgtccgc
     5701 gaaactcaca cagatcttca gttccagacg ccgtgtggcg ttggccgagt aattgacaat
     5761 gttgtcaccc atcatcttgg cattgggaac gatgatcgtc ttgttgtccg gggagcgcaa
     5821 ttcggtggtg aagatcccga ccgccttgac cagtccgccc acgccggcac cctcgatgta
     5881 gtcgcccgcc cggaagggct tgaacaaaac catcagaatc ccgtaagcga agttggagag
     5941 cgacccttgc agcgccaagc ccaccgccag ccccgccgca ccgagcacgg ccacgaacga
     6001 ggtcgtctgc aagccgactt gacccagcgc ggtgatgatg acgagaatca tgaccgcggt
     6061 atggctgaga ctggcgagga acgtgaccag cgtgtcgtcc agatgcgctt tacgcatgag
     6121 gtttttgagt gcctgacgga tcatccgggc gacgaaccac ccgaccagca ggatgccgag
     6181 cgcaaacagc actttcacgc catacagctt gagcgcatcc aaaagctttt gtccgatcgt
     6241 ctccatcatg aattccgcgt tgccgaatta tccccacgaa tcagaaatgg gcgaacttta
     6301 tcaaagcgaa cggcaaaaac caagcctttg atgtgacgct tcggtttgtc cgggcgtatc
     6361 cttgagtcac tggccgttga ggcgggcagg gttcagcaga cagatagata ggccgtgccg
     6421 ggtttcggca gggatacctc cccgaggcgt ccgcggacga gaacgaggac ggggatgggg
     6481 aaacgatagc gatggcgatt tcaagaaacc agaaaccaaa gtgcgggaaa catccttcgt
     6541 ctccatcgtg tgcctcggta acaaaaccct tgcgcaaaag cgtctccttg gcctattgtt
     6601 tgcgcatatc ggcctacgtg tgcccgcgcc cgaccggatc gccgcgcgga cacgcgcagg
     6661 attaccgtat gtgcctcaat gattatccta ggggagaaac acaccatgtc caccctgacc
     6721 agacgcacct ttcttcgcgc cggaggcgcg acgaccgcag ccattaccct gccgaacctg
     6781 atccccccgc atctgttcgg tgccgctgcc ccaagcaagc agatccacat cggcatcatc
     6841 ggatgcggac ggatcgcgac cagcatggag atgcccggac ttttcggctt gaccgagatg
     6901 gcgcggatca cggccctctg cgatctcgac tccaagcggc tggcccattc gaaggcccgg
     6961 gtcgagaacg agtacaacaa gcgtctcggt ttgcaaaacc ataccgtcaa aactttcggc
     7021 cgctattctg atctgttgcg cgacggcggc gtggacgccg tgatgatctg cgtgccggat
     7081 cactggcacg ccttgtgcgc catcgaatgc gcacttgcgg gaaaagacat ctggttgcag
     7141 aagccctttt cacaaacgat ccatgagggg cgtattctgg ccgaaatcgt gcgccgcaaa
     7201 aaggtggtgc tgcaggtcgg gtcccagcag cgttccagca aacagttccg cacggcgtgc
     7261 gaactggtgc gtaacggccg gatcggccgg atccgccgcg tcgaagtcgg tttcggcaaa
     7321 gaccgggacg gcggccgcaa agaagagatg cccattccgc cgaatctgga ttacgacacg
     7381 tggctggggc cgacaccgga ggcgtactat accgaggacc gtgtgcataa tcaggacatc
     7441 gcccaaatcg cttcccgtcc cgggtggata cagatggaac cctacggctg gggcatgatc
     7501 accaactggg gcgcacacca catggatatc gtgcagtggg gactgggcac cgaggagact
     7561 gggcccctaa aggtttcggg cacctgccaa tggctcgaca aagggctctg gaatgcgcac
     7621 cgcgaattcg acctgctcta cacctatccc gacgacatca aggtcagtgt ctgttaccgc
     7681 tatccgaacg gcgtgcgctt catcggcgat gaaggctgga ttttcgtaag ccgcggcgcc
     7741 gccaaagtca ctgccagcga ccccaccacg cccggcaaga ccctcaaggc gctggatgcg
     7801 agcgacccgc ggctccttga agcgccgctg cacgctaacg ccgtgcgtct gcatgtcagc
     7861 gacaaccatg tgaaagactg gctgcttgcg atccaaaacc gcacaagccc ggtgaccaac
     7921 gctgaaatcg gccaccgctc gacctcggtc tgcaccctcg gccatctctg catgaaaacc
     7981 ggcaagaccc tcgcctggga ctggcgtacc gaacgcaccg atgacgccgg ggccaacgca
     8041 ttgctggcct gtcccgagcg cggtcccttc agcatcccga aagtgcttca ggctgccgac
     8101 atccgcgtca gcgtatgagt ccgttacact ccgccttttc ttcaaaccat cccaacgagg
     8161 acaccatgaa tcgaaacacc ctgctgctca ccggcgccgc gctgttggcg ctgaccggtt
     8221 gcgacaaccc cggcacccgc tgctgcccca acacctgcgg gggcgacacc gtgaaactga
     8281 tcactctgga ccccggccat ttccatgccg cgctggtgca gaaacgcatg ttccccggcg
     8341 tctcgccgaa ggttcacgtc tacgcaccgg ccggccccga cctctccctg cacctcgacc
     8401 ggatccgggg cttcaacacc cgcgcggaag acccgacctc ttgggagagc gttgtgtaca
     8461 ccggtcccga cttctttgac cggatgatcg cggagcgggc cggaaacgtc gtggtgctgg
     8521 ccggcaacaa cgcacgcaaa accgaataca tcctcaaaag cgtgcaggcc gggttccatg
     8581 tgcttgccga caaaccgatg gccatcacgc ccaaggatta cgagttactc aaacaagcgt
     8641 tcgaagtcgc ccgctccaaa ggggtcctgc tctacgacat catgaccgag cgctttgaga
     8701 tcaccagcgt gctgcaacgc gaactgtcac ggttccccac cgtgtacggc gagcaggaga
     8761 agggcacacc ggacgatccg gcggtcacca aggagagcgt ccaccacttc tgcaaacagg
     8821 tggccggcaa gccgctccag cggccgcctt ggtattacga caccacccag cagggcgagg
     8881 cgatcgtgga tgtgaacacc cacctgaccg atctcatcca atgggagacc ttccccggcg
     8941 tggccctcac gcctgcggac atcaaagtgt tgaaggcgcg cgtctggccc acgccgatca
     9001 cgctggaaca gttcaaaacc tcgacctcgc agaccgcctg gccggattac ctgaaaaaag
     9061 acctcgacgc ccaaggcgtg ttgcagtgcc gtgccaacgg cgaattcacc tatgtgatcc
     9121 ggggggtcca tgccaaagct tcggtgctgt ggaatttcca acccccggcc ggcgcggggg
     9181 acacccatta ctcgctgatg cgcggcacac gcagttccct gatcatccag cagggcgagg
     9241 ccgaagggta caaaccggtg ctctatgtcg aaccccgccg cgtcgcaggt gtcaacaaag
     9301 aggaactcgg ccgtgccctg accgatgcag tcgcaacgct cgccaagacc tatcccggca
     9361 tcagcgcgga accgcatgaa accggttggc gcatcatcgt cccggagcag tacgcggtcg
     9421 gtcacgaagc ccatttcggc caagtcatgg aagccttcct cggttttctg cagaaccgtg
     9481 acatgcccga ctgggaggtt cccaacatgc tggtcaaata ccacacgctg atggaagcct
     9541 atcgcctgag ccgctgacca ttcacaaaaa gtgcgccccc ggttacggtt gggcgcaccc
     9601 ccgttcccct acccctggaa gcttccggat tcggcaacgt cctcgggcat ccgagacgtc
     9661 caaccgtact ttgcgcgagg aaatcactcc gccgcttttt gggctttgac acacccttcc
     9721 gccgttgccg ttagtttcca tttcgcgccc ggctcgatct ccagaaaaat acccgcccgt
     9781 ccgaacttga gcggatgcga atctgtcttc atgaaacggt catatcccct cggatcggaa
     9841 ttgccgacgg cgagaattcg gcatccttcc acctgaaaat ggatgcggcg gtcgtcgtcc
     9901 ggcacgaagt tcccgtcctt gtccgcaagt gtcaccatca ccacctgcaa ctttccgtac
     9961 cgcttcttct ccggtgtcag caccaccttg acaggcacac ccgccgtccg tcgtacctgt
    10021 tcgccgagtt ttctcccctg cgcgtcatac gccacggcct tcacctcgcc cggctcgtat
    10081 ggcacctcca tccacatgag ccgataccgc ggcagaacgg agtagtagtc gtcgagggtc
    10141 cccgccttag gatccttccg ccgacgcccc atgctcttcc cgttcacgaa cagttccgcc
    10201 tccggcgcgc tcgtgtagac gaacaccgga atcttcttcc cgatcttctt cggaaagttc
    10261 cagtgtcccg gcacaatgtg aagcgtaaag tcctccgtgt tccagtatga acgatagaga
    10321 taataccgat ccttcgggaa cccaagcaga tcgcagatac cgaaatagga actgcgggaa
    10381 tccttgaacg gcgtcggttc gcccaaatag tcgatgccgg tccagacgaa ctcgcccgaa
    10441 cagtacggat ccagttccat ccgcacgaat tcacggtcgg ggacatcgct ccacggcgcg
    10501 gcattgaaat cgtacgagtc gatgcgatgc acctcatgtg cgtagttcgt cttgcaggtc
    10561 ggcaaatcgt ccgcatagaa tccgaactcg gaaaccgcag acgcgctttc ggaacagagc
    10621 accggaatct gcggtgccaa ttcgtgcgcc ttggcgtaga ccctgccgta attccatccg
    10681 gcgatatcga gatcaagata gtcccccctt cccgtaagcg catggccgaa cgcgctaccg
    10741 atgccgaccg gacgtgtccg gtcctcctcc cggatcgcgt gccggtactg cgcgcaccgc
    10801 tcgcggttcg tccccagcgc aatcggaccg atccaccagc ccacatcccc gaacttcgtc
    10861 gcacgcgccg tctggatctc gttccccatt gaccacacga acacgcacgg atgcagacgg
    10921 tctcgccgca ccagctgccg cagatgtcgc gcgatgaact cctcttgcac gaagtcgccc
    10981 ttcaccgccg aatcatccca cttatcgaaa cactcgtccc acacgaagat acccatctcg
    11041 tcgcacgcat ccagaagttc cggggccggc ggattgtgcg atgtgcggat cgcattcgct
    11101 cccatctccc gcatgatatc gagctgacgc cgaatcgccc cttcgttcac ggccatcccc
    11161 agcggcccca gatccgcatg ttggttgaat cctttgagtt gtatccgctc cccgttcagg
    11221 aagaacccct cctcgggatc aaaacggaat tccctcaacc cgatccgccg cgttagttcg
    11281 tccgcatatt cgtccgccac gatcgcgatc ctgagcgtgt agagcttcgc cccgcgctcc
    11341 atttgccaaa gctgcggatg gggcacctcc agtgtccgtt cgaaccgcgc ggacgcaaac
    11401 gcctccagtt ccatttccgt ctttcccgtt gccgccgtcc gaccatctgg atcggttaac
    11461 tcgaatgaca ccctcacaag gcggttcgtc gctgcccgcc ccgtcacctt gccgctcacc
    11521 ttcacttttg cgaggtccgt cagcacgtcc accgtttcca ccgcaagcga atcgtcctcg
    11581 atatagacgt tgtccgtcgt cacaatccac gtattgcggt acatgcctcc gcccggatag
    11641 aagcgtgagg tcaactcctt tgtactgcac cgcacctcca cgtcattcgt gcctttcatc
    11701 agaaaacacg tcgcatccgc acgatatccg agatagccgt agtcgccccg accgcagacc
    11761 tgtccgttca cgaacaccgc cgaccgtgcc atcacgccgt cgaagtcgag gaatacccgc
    11821 cccttcggct cctccgccat cacaagtttc ttccgataga cccccatgcc cttccaaggc
    11881 agtttcgctg tgtcgccgtc gccctccgga tcgaagggac cggcaatcgc ccagtcgtgc
    11941 ggcacccgcg catactcgaa cgcccccccg tccttcgcaa actcccatcc attgttgaag
    12001 ctttcgaccg tccgcgcacc aacggacaga gtcgcgatca tcaaaattga catcaaccgg
    12061 aactttttca tcgtcgcccc cgtttacttt cccggttcgt acaccaacgt atccggcatt
    12121 gccgtcttct ccgtcaccgg attcccgaag acatcatgca ggcatgtctt cttcgcaagc
    12181 gcgagtttct ccttctcgga aaggtcggaa tacaccctcg cattctttcc gtccttcgcc
    12241 cggaaggtga atacacatcc cttctcaccg aaatcttcct tgttcacaaa cgtcttgccc
    12301 tcgattgtct gcgccagtgc cgcgtgcgcc acgaaggacg gcaccggata gccatccttg
    12361 ccaatcagca cgctaaactc gctgcgccgt gccggatgca cgtatccgtg acaggtgtag
    12421 aggaagacct ttttgaagcc atccgccagc agttgtacga gaaaccgcgc cgtgttgtcc
    12481 gaaagctcaa gataatcctg catcgtatcc ggcgtccacg gcacggtcgc gtgatagagt
    12541 ccggtgttgt ggcaccgcag cttgctcccg ccgtcgttcg tcccctgccc ctcgctcatg
    12601 taaagcggct tcccgccaaa atccggatac tttgcacgga tcgacggcag cgaatacttt
    12661 tccatcacgt tcgctgtctc gcggtgcgca agggacggca cggagtagta gtgccagtcg
    12721 ataccgtcgc agcagtggaa accaccgccg tccatcacgt cctcgaccca ttccttcgga
    12781 ccgcccgcat tgaacccgaa gagcttcaac ttgggatcga ccgccttcag cgcgtcgtag
    12841 gtctggcgtt gcagtacggc gaagtcctgc cccgcccgac ccggtgcgaa ccacttcgcg
    12901 tcgtcgttga actgccagct gtgtccgccc ggttcgttcc atacgaagta ttcgtcgatc
    12961 acaccccgat agtgtttttc gaacgtcgtc acatagttca cccagtcaac cgaattcgtc
    13021 ggacgcagat aacgcccgaa ataccccggc gtccacttcg gattctcctt caccaagtct
    13081 gccgaatggt tcgcccattc gggcgacatt cccagttgcg cgaagatttt gacattcgcc
    13141 ctgcggtact cgtcgatcgc cgcgtcccgg aacgtccact cccccttctt ccgttcgaga
    13201 tagaaccagt tggaaagatc ctccgccgcg tcatggaacc gtacccagtt caccccgcac
    13261 gccttcatca tcttcaccac gggacgccac ggattgaagt ggcttgcgaa cggtgaattc
    13321 ggcgcgtcct tcccccacat caccggacgc actacacgcg taaggacgat ttcctccctc
    13381 tccgacacga ccttgccgtc cttttcaatc gtcgcgcaaa tccggaactg ccccatcgga
    13441 cagtccttga acgccgatat ttccaacgtt cccttctcaa gcttcccgcc cttgagcgga
    13501 atctccgcaa tcgtcacctc ttcgccgtag agatccgccg cctccacttt catcaccgac
    13561 cccgtcggca catcgaccac acaccatttt acgagcggct tctcgtccag gaactgtacc
    13621 cgcgttcctt ctcccgcatc gccttccacc actgccatct gcaggcaggg acgcgtatcc
    13681 cgtacacctg tgccgtccat cggtcgtaca cgcacgtcat caagccacat cgttcccgcc
    13741 tcaacaccgg ttccccatcc gatcgtgaac gcctccgcga gttcgggccc cctgaatcgg
    13801 aagcgatgct ccttccactc ttttgtcggt ccgagccagg attcgccgaa catctctccc
    13861 cgccagtggt cgtcgttgac gaaattccgc acacggcaat cgctccggta cttgaacgac
    13921 acctccatct cgccacccgg aaacgcagtc tggaacgctt cggaatagaa ggtccacatg
    13981 cggttgcccg aaaccttcaa caccggcacg ccgtccggtg ccggttccgt ctcgtccgca
    14041 ccgtacaccg ttccctcgca atcccgccgg aaacaccacc ctgtcggcaa gcccaacggg
    14101 aaacggttgt ggcgaatgta ctccgtcacg tccttcgacg gacgccgcac cgcctcctgc
    14161 gccatctccg ctatcgtcgc cgtttcaacc ttcatccgct tcaggtctac cacgcccggc
    14221 ctcgtgttca cgaacagcat cggcaactgc ttccagtcct tcggcacgga gaagcaccaa
    14281 ctctgcacgc tccagttctc gctcatgacg tgccgactga gacaatccga atacggcgat
    14341 ccgcccatcc gaaacgaaca gcgatacgtg accgcgccaa gcgtgcgcga ctcgaccgtg
    14401 aaccggtaac agcccggttt cagttccttc gggaccacga attgcgttcc gcccttcgtc
    14461 tgcgcggacg tcgtcaggcg cagatacttc cctatgccct cctcactctt cacctccgtc
    14521 gtcgcgatgc cgtcgcacca gcccgtgtag ttctccgcaa agtcatccgg cagtaccccg
    14581 tttatcttcc ccacgccatc gcgcgccacc tgattcgtcg ccgacccgaa gtcaatatcg
    14641 tagacaattc cacccaaggc atcacataaa atggaaaaag agaaaaggaa aaagaaacac
    14701 gcgataacgg cagggcgaca tgaatggagc cgccacgacg gtttcatcga aaccgtcctg
    14761 ccggcgtttc catacattgc ctccgtcagc acggcattca agcatacagg tgtcaacatg
    14821 gtgtcaattc tacattcgcg cccacgccgt cacggaccac gcggcgagtt tcggcagacg
    14881 gtaccgcgtg tacgcgcctt cccgcacacc cctgatcacc gtgccatcgc cgccggaaag
    14941 acggattccc ttcgaccacg cttcaccctt gagtagaact tccattccct ccgccgcatc
    15001 aaaggacatg ttgtagatcg cgatcgcccg gtcaccgcgt acccacatcg ccgccttgtg
    15061 gtagctgtcg atgtatcccg tgagcgtctc ccgcgagagc caccgcatca cgttcttgcg
    15121 ttcgcgtacc accggactga acgacagccg atcccaggcc atcgtccccg tcatgaagat
    15181 ccgtccccca cgcctgttct cgtacacgcc gctcacacac ttcgcgacga cacgtcccgg
    15241 gatatccacc gcgtccgcga gaatctgcgc cccttccatc ggctcgagtt caatcacgtc
    15301 cccgccccag aatgactgcc gcatgtcccg tcggaatccg gccacgcccg cgttgagtgg
    15361 atggtcgacg aagcgttcgc gcgcgtcctt cgaaaccgcc ttgccgatct tgaacccgag
    15421 atcttctccg tagccgcggt cgatgatcgc ctgcgccgcg tcgagcggca ggtagagccc
    15481 gcccgcgaga cggcgttcga gttgctcctt cgtccaagac cacaccgcct gcgcgttcgg
    15541 cgcgcacact tcacactcct cctcgcggta ggcggcagga agtcccgtca tctgcagttc
    15601 ggactgccag aaccctttct cccaccactc ttcctgcatc cattcccctg tccgtccacc
    15661 gttcttgttt cccacgaagt aatcgcgtcc acgcccgttc cacacgcccc tcggcttcgc
    15721 ccgtccgcac gccgccacaa tcgcgtccgc ctctacgcgc accttctcgc acgactcaat
    15781 ccgcggcgcc accgcctcgt aggtctcgtt gtccgcaaaa ggaaacacgt tccacgccgt
    15841 gccccgcgtt cctgccgcca tgtatgcgag cgactccgtc ttcagcgaga aattcgcctt
    15901 atgaaggagc tggtactgga aggtctcgat ctcagattcg ttcaccgcca cgccatccgg
    15961 cagatatgcg ctttcgtagc ccatctcgtt cgccttggaa agaactccgc cgagtccgcc
    16021 gatgtcgttg tagtatccgc cgcccggacg gaagaacacc tcctccgtcc ccgtcggcgc
    16081 gagcgcacgg aacttctcct cgtagttgtt ccccgtgtag gccgtgatcg aatccatcac
    16141 gccgatcacg atcttcgggt caacggcgtg cacgacctcg cggatgtact tgagcagatc
    16201 cgcatacacc tcgcggttga gcgtcatcat cgcccgccgt cgcgcctcgc ccttttccgc
    16261 gtcggtaaag aacgcgttga gtcccttcac gtcgccagaa taccccagcc ggtcatggag
    16321 acgcttcatg cagatcgggc agaagcaccc cggacccggc acgccgtgcc ccgagagtcg
    16381 accgatgtcg tcgtccgtcc agatgaagtc cggccccgtc tccgccaaaa ccgtgtatat
    16441 cggcttcacg tacttctccc gccacaccgg atcgagcgga caccgtgcac tcttcgccgt
    16501 ctcacccttc cagttcacca gatactgcgc cccttcgatc acgatcgccc ggtcgagatc
    16561 ctcgtccgtc tgaccgatcg tgcaaaggtg gttgatgccc gtgcggtgtc cgcgtgcctt
    16621 gaaatgcgcg atcgccttct tcaattgcgg caggcacggc agaaagtgct cgaacttcgg
    16681 cggcacgtgg cttggacacg taaagaatgc gatctttccc gtcacaggat gctcgtcgaa
    16741 gaacttcgtc aatttcttga actcctcctc cgtccgccac tggttctcgt ggatgcgcca
    16801 cgttaccgtc gcagcatgcc cctcgtcgcc ctgcgcaaat gaaggattga aaatgaagaa
    16861 tgtaaaacta aggacgagga gcacgacggc gggaacacgc gttccgcgca cccacggtcg
    16921 cgcaggacgc acagtcctgc ccattgccct ctccgtattc aaacaatcag acatcaccat
    16981 gacgtttccc atttcccgtt ttcctttctt catttctcat tccccttttc acattcttca
    17041 gtagttcggt ccgtagtgcg agaccttgaa cggacggatg ccgagcttca gcgccggcga
    17101 atcgggcttg aacgagtagt cgccgcgtgc cggatcgacg aagagcggat cggcgaccac
    17161 gctgtggagg tcctgaccca tctccgccca tttctcagcg gggatctcct tgaagttccg
    17221 tccgtaacgc accctgttcc ccttcccgca ccagatcacg ttacggtcga agagcgtcgc
    17281 cttcggcgcg aactgtcccg gcgccgtgta gaaggactcc ggctggtcgg gatagtagac
    17341 gacgttgttg acgatctggt tcccgatcat cagcatcccc tccgaatgct cgaacgccgt
    17401 cggcggcagg cgaagcgacg gatagttcgt ccacgccgca ccgcccttca tcatgtggtc
    17461 gtactggtcc tgcgcagccg tcttcaggta gggcggcgcg ttcgtggtga agccgttcat
    17521 cgtgatctga agccggtact tgccgaatcc tccgttcgaa atgaagaagt tgttggagac
    17581 ggtgttgaac cgcgccgaat gaaagaatat tgcgccctgc gtgcagcgtt ccacgaggtt
    17641 cccgaccacc tccgcgccgc cggaggagtc gtcgaggtag ataccgaacg catccgcctt
    17701 gcgatgttcc caaacacctg tccccccgtg gctgaacccg taggagtccg agatccagtt
    17761 gtcgttgatc ttcgtccccg cattgttgac gtatccgccc gtgtagatcg cccctgtgtc
    17821 ctcgctctgg aagttcgtgt cgtggatgcg gttgcgcgag atatcgttca tccgaccgcc
    17881 gaagctgatc gccccgcgcg gcgcgtggtg gagaaggttg ttcacgatcc gcgtgccctg
    17941 tcccgcgccg catatgcact ctccacagcc gttgatgcgt cccgtgtggt ggatgtggca
    18001 gaacgacacc tcgttgccgt ccacgtgccc cggagcgtat gccgcaggag aggcatcccg
    18061 caacgaatga acctgcacgc cgctgttgcc cgtgttccag acccggcatt cgcggatcac
    18121 gatgcccgaa ccccacgtct ggatgccgca tccggcgaac cagcccgtgt cgtggatcgt
    18181 gcagtgcgtc accgtgcagt tgcgcgaacc gtcgtagagc ttgatgccgc cgccaccctc
    18241 gcagatctcg agatcgcgga aatggacgtt ccccacgtcg ttgaagttga agaccggctg
    18301 cgtcgtcgtc gtgatcgcgg tgcggacctt gttcgggtcc atccccgcgg gcgggatgaa
    18361 ccagatccga tgcgcgtccg cgtcgaagta ccattcgttc ggcgcgtcca gctcctcgcg
    18421 gagcccgatc aggtagaagc ggtccgccgg gcgcgggaac tcccccgtgt ctcccgccag
    18481 atggagcgtg tgcgtcgcct cgtccgccgc cgcgatcggg ggtatccggt tccaccagtt
    18541 gtagcgcggg aagatgttca cgcgcccttc ggacggtttt gcccacgtct tccagtcctc
    18601 cgcccgcatc ggaatgcggt ccttccgctc gccctcccgc agtgaataca tcgaaaacat
    18661 cttgcctgga atgaacagcc agccgcccgt ctccggatgc tcaggatcga agttcggata
    18721 acggcattcg gtccgtatct caatgtccgc gtggaacgtg tagatccgct ccacgtcctt
    18781 cggcacgtcc gcgcaccatg tgtcggtgcg tccgttgaac gcacatttcc tccacggccc
    18841 cacgcgcgta ccgcccagca cccgcgcccc ctcctcgccg cggaacatcg tcgccgcctg
    18901 cccgtcgccg atcggattct tcgtgcggtg cagaaacagc ggcaccgtga tctcgtggtc
    18961 gcccttcgca aagacgatgt cgatcggctc cgtcggcttc tgccgcctca ctgcgtccgc
    19021 ctgcgcgacc accgccttca cgtcatcgcc cggcagcacc cggaattcgg cggcgttcaa
    19081 atgtgaaagg gaacatgcaa aaggggaaac gacgagtacg acaaacgtac aggaattcat
    19141 gattttcgaa cttttcattt ctcttctttc atttgatcac cgacttgccg cataagatcg
    19201 gcggtgtcgt tttccttccc gtcccccgtg acgtcggcac gcacgcgccc cgccttgatc
    19261 cggcgctgga tctccttttc gaactccgcg attttcgcct caaacccgtt gattttctct
    19321 tttccatttt caaattgata ttcgaatgcc gccgcttgct tcgcaagcac tttctcgcag
    19381 gcggaaacga catcgtccgt cggagcatcg ccggtcatcc gcttcggatc gcggatccag
    19441 ccctcttcga aatccgccat cttcgagtaa aacgcgtttc ctcgatgctt cggacgttgc
    19501 cagatgtccg tcggcggcac aatgtctgcc agttccgcct tcccctccaa caccttgcgg
    19561 gcatgttcca aaaacatctt ccaccgtggc aggtaatacc ccgagaggag tccgccccat
    19621 tccttccacg catactcctg aatcggatcg ccggcgaccg tcggcacgcc gaatggcccc
    19681 cagtaggtca cctgcaccgc ctgattgaac gtgtagagat tccgctcctc gtccgtcttc
    19741 ccctgtgcgg ccgccagatc aagatacgtc ttcaatgagg agagcggctc atgcttccgg
    19801 agccagtcct cgtccgcccc cgtctccaaa aacgccgcca ctgcgcagtc gtattccttc
    19861 acgtcccctt tcaggaacat ctcgaccacg tccacaaact gcgcgtagga aagatcgctc
    19921 aagagacgat gccccgcgtc tacgaggtcg aaccgcatgc ctggacgcac gccgaattcc
    19981 tttgccgccg taatcatctc cctccatgtc cgcaggaggc gccgatggtc gtatccggac
    20041 ctgaatccca cgttcggatc gcagcgtttc agcaggaagg tcggtttggc cggatagacc
    20101 gcggacgcga gatggacccg tccataatag gtcgcgagaa gtccttcggc aaggctcttc
    20161 gccgtagcgg aaggcacgcc gtagcgggcc tcgagcagtt ccgtcgtcca ggcgagtggt
    20221 tcggggttgt catcccgcca caagaggtcg agtgcctgct cccagtagag cggcgaggtg
    20281 tggatgccct ccggaaaata ccccatgccc acgcagttcg ggaacttccg gtatgtctcg
    20341 gcgaagtggt tttcagcgag ccaacggatg tcgccgcccg aggccgtccg tccgccaaag
    20401 tttatgattg cccccgtcac gaacggatac ccccagaaac ctttggtgtg gcggcccttc
    20461 tcgcccgaga tgtccaggac aatgatgccg tccttcggaa acgacttcag aatcggcacg
    20521 cggatggacc agctctgcat acacgccttc gccgcaggat ccgctgcgcg gagctcacgg
    20581 ccgatcgtct cgcccaccgc cctgagatag tcgtcccccg cctgcggcgg ctgcgattcg
    20641 tggaaaggat ccaccgcgta gtatccgtgc agaccgaaga gcttcttttc cgcggcataa
    20701 aacgcccgcg cgaatttcgg gaagagcggg tcgaggggat cgagctgcgc cgaccccggg
    20761 aacccgcacc agggcggctg gaacttgatc ttcgcggacg ggaacttctt caccgtatcg
    20821 cgcggcacgt tccccgtgaa tccctgctgg atgggcgtca tcccgagctc gcgctcgcgc
    20881 gcgagaataa aacgcgcgag cgcgagacga ttttcgatcc aactcttcgg caacggtccc
    20941 gcatagctct cgatgttcgt catccactgc cacgccatga aggccgggcc cacgaggaag
    21001 ctccgcgcct cctcgtcgct ccatccaagc gacatcagcg tctcgtacca cacgcactcc
    21061 acgcccgtgc aggcgagcgg cagattgacg cccctgagcg caagcaggtc aagttcccgc
    21121 tcccaccgcg ccttgtccgc gaacgcgagc gaatacgaat gcgtgcagaa attgaacacc
    21181 gcccgccatt ggtacggcgc ctccgcccga atcacgccat ccatcctcgg gggggcgtgc
    21241 cgccctgcga ccgccgccgt gccgccgccc tcccagaaat acgggacctt cgccaccttc
    21301 cggagatact tcccgtatcc cgcacactgc gcgttcggcg tattgccctt taccgcgatg
    21361 cgcccgtccg gcagggaccg tacttcgcag aactccttct cccccgcctt tcgcgccaca
    21421 tcctcgaaca cgactttccc ggcccactcc ggcgtcacac gcgccaccag cccctctgga
    21481 cccggaaatg cgttcaaatg gaaaatgaga aacgcagaat ggagaatgaa gagtacgatc
    21541 ctaccgtttt tccattttcc atttcccact ctccgttctc cactcttcac cgaaaaccac
    21601 cggtcattcg atttccaacg ccacacggaa accggcgaag tacgtgctcc cgttccagtg
    21661 ccatccgccc atatcacctt cggagggctt catcttgttc cggaaaccgc tgtgattcgg
    21721 cgtgtggttg ttggtgaagg ccgccggccg cgcaacacgg tattcgcccg atggcgcgcc
    21781 ctccgggtcc actaccggcg tctcgtcgtc tacgaattcg taggggccgt accagtcgcg
    21841 gcagatctcc atcacctgtc cgtgcatgtc gtagatgttc cacgcgttcg gccgcttgag
    21901 ccccacctcg tgcgtgtgcc cgtcggcgtt gtagttgatc cacgagactt cgtcccagca
    21961 ggtcccgtag ctgacaccga tattccccgt cgtacccgtt cccgcacggc aggcgtactc
    22021 ccattgcgcc tcggtcggca gatcgaagtt cattcccgta tacgcgcgca gtacaccgaa
    22081 gaacgaaccg gcgtccacat cgcccgatgt cggccattgc gccccagccg tcttgccccg
    22141 aagatcgtcg tagttgatac tctccatcgg atggaccgcc caatcaatgt ttgcggagta
    22201 ggtgtatctg caccgcccgc gccatccaac ccccgcaagc gcggtgaact ggccctgtgt
    22261 gcagggataa acgccgatgt agaagtcgtg cgtcaacttc acgagatgcg ccacttgatc
    22321 cgccccgaaa aacccgtcgg aggagggcgt tcccatccgc cacacgacct cggccgccgg
    22381 acacttccgg aagatcatgt gcgtcgtctt gtaccgagca ttattgaagc cgcctggaat
    22441 cgaattcgta ttcgcgtagt aaagcggaac cacgttcgtc gccccctgag gacggtccat
    22501 gcggatcacc atgtagtcgg gcggcgtatc cgtgcgccag gccgtgacca ccggcttcat
    22561 gtctgcggtg cccatgaccg gacgccacac gatcgaccgt gtgccggtca caaccttcct
    22621 gttgacgtcg ccccagacct tcgtgaggtt ctcaccaccg atcgagacgc cgttcgtcgt
    22681 cacgtccagc gtgacaatcg cgtccgcgcc cgaaagcgtg tagccgatcg tcacgttctt
    22741 cgtcgtcgcg tcctgcgtca tcgtcacacc gctgacgacc gggtccgccg ccaacgccgt
    22801 gaatgcagca aacgccagca acggcaggac tcgtcgccct gcgaccgccg ccgccatttt
    22861 ccattttgca cgttccattt tgaattctcc cctcgcctca cttgaacagg aacagcgtcc
    22921 cgaatggatc gctccggaga ttgatgccga gcgactcgaa aaacgctgac acgaacgcgg
    22981 tcagtccacc cgcaccgctc ggcgcctcct gcgacgtgac ggacgccacc gccaccgcgt
    23041 ccgtcgtcgt cgcgctcgcg gtcacctcgg caggatgatc ctcgatcgtg aacgtccacg
    23101 ccggctcggc caccgccgcc atcgccataa acacggcgac tgccaccatt ttttctttta
    23161 cattttccat tttacaccct cctcattcac ctgtgatctc gcacacaacg cggaatccca
    23221 ccgaaccact cgcactcccg gacggatcga tatggttacg aaaggtgtag cgatggacgc
    23281 tgtgcgtgga gttgtcgtat ccgcccggac gcatcacgcg cgttgatccg gatatggggc
    23341 cgagcggatt gaccaccggc gaaccgtccg ccggaaacgc gcagacctgc tcgaaccagt
    23401 cgcggcacat ctcccacaca ttcccctgca tatcgtagat tccccagttg ttcggcaact
    23461 tggttcccac cgcatgagtc cgcttgtttt cgctgccgcc gttgtcaccg ctgttccaac
    23521 ccgtccaggc atagggattc atgatctccc aacccgacgt atacgtatcc accgccacgg
    23581 tcgaggacgc cagttggttg ccggcccgcg cggcgaactc ccactgcgca tctgtcggca
    23641 ggtcgaagcg catgcccgtc cggttctgta gaacggcgaa gcaggaatcc gcatcgaatt
    23701 cgtccgttgt cggccactgc gcgcccttcg tcgcgccacg cagatagtcg tagttgatgc
    23761 tctccatcgg atggctccac cagtcgtcgt gggaagcttt gttgtaattg cagccgccca
    23821 cccagccaag accgcgaagt tccgcaatct ggccctgggt gaattgatag acgctgatgt
    23881 agtagtcgtt cgtgaacgcc acgagatgcg gcgtttcgtt ggctcccgtg tattcgcccg
    23941 agttgcgtga gttctcgccc atacgccacg tcacgcctgt cgctgggacc ttgcgcagga
    24001 ggagatgggt acgcttgtac atgtcattcg taataccccc ctcgattgcg tctgcacagt
    24061 cgtagtagcg cacgcagttc gccgtgtcca gactcgccgt catgtacagc ggcggttcgc
    24121 tcaggctcca tatcttcacc accgccttcg cagacccatc ggagacgaca ttgttcttcc
    24181 acgacttgtt cggattccac tggatggcgc gctgacccgg cgacacacgc ttgttgacgt
    24241 cgcccacaag accgcggata tttgccgcgc caatcgaaac accgttcgtt gtgatatcca
    24301 gtgtcacgat cgcgtccgca cccgtcagct cgtagccgat cgtaaccaag tgcgtctggg
    24361 aactctgcgc catcgtcacg ctgttcgtca ggatcgaggg ctcggccccc cccgccgcca
    24421 acgccaggct aaacaccgcc gtcatcccca ccactctctt catcttgacg tcctttctga
    24481 ttggtcaccg ccggtcacca ccaaggcaac acccatcacc caagccagat cgacgaaacc
    24541 gtccgtatga tttagttgca gtttatcaaa ttccatttcc cgccaccatg tcgtaatgcg
    24601 caaaataaaa tatcaaaaaa acgaaagcca accttgttcc cctcacagga tcttgatatt
    24661 ctgacgtcca tggtactcac aaccgagatc ccccgggttg tcatctgctt ctcggccgcc
    24721 atcaagacaa tgcgcgagtt gatgcagggc gtgctcgaat acgccaaggc caacggcccc
    24781 tgggccttgc acatcctcgc cggcaggaat ctcgaacaga agctccccga cctcgtggaa
    24841 tggggatgca acgggttgat cggcaacctc tccgacgaac agttcgtaga catgaaaatc
    24901 cccgccgaca tcccgctcat actcaacggc cgcgccagca acgaggacga ccgtccggtc
    24961 gactacccgt ccaacaccat cggtctcatc gaatgcgaca atgtccctat cggacgtgcc
    25021 gccgccgagt tcttcctgtc caagggtttc accgccttcg cctacgtcgg cgacgtcaat
    25081 cgtccccaat ggtcgattcg ccgcggcgag gtcttcgccc gatgcgtagc cgcacgcggt
    25141 ttcgagtgcc atcattacac gctgccgccg atttccgtcg cgcgcaatgc cgtacgcgaa
    25201 cagcgtacgc tcgccagatg gctcgatgcc ctgccgaagc ccattgccct gttcgccgct
    25261 aacgacactc gcgcccggca ggtcgtcaac ctttgccttg agaacggcat cgccgttccc
    25321 caggaaatct ccattctctc gtgtgataac gacgaactgc tctgcgaatc ctgtgtcccg
    25381 tccctttcct ccatcaagat gaaccccttt caggcgggct acgaagccgc ctcgctgctg
    25441 gaccgcgcga tgcacgaggg gttccggcgt cgccataggc cgattcgcgt gacgtacacc
    25501 ctcgattcca tcgtttcgcg tctctccacc gcagatctgc gcgtgaagga tccgctcgtc
    25561 gagcgcgccc tcacctgtat ccggctcaac tccgccgccg atttctccgt cggagacctt
    25621 gcggagaaac tcaaggtctc acgccgtctt ctggagatgc gtttccggaa ggtgctcgac
    25681 agcacggttc acaacgcgat catgtctgcc cgcctcgaac gtgtctgcga ggtgctgaaa
    25741 tccacccggc tcccgattca ggacatcgcc gaccgcctcc acttcagttc cgccagccac
    25801 ctcggcatca tcttcaaacg gcaattcggc acgacgccgt cacgtttccg cagagagttg
    25861 agcggaggtg gacgcaatga cttcagattg atggcaggcg attctaccac cgtgtcccgc
    25921 atttgacgga tgcgcgtgat ttctgggcac cgcgattttg gctacggtgc ccatgacgat
    25981 gacataccgc gtaccgtaaa gggacacccg ttacggttgc ctttcccctt ccggcctgtt
    26041 atgatcgtaa tactccgttc gcatggtgcg gacggtttaa actgattaag gagttgttat
    26101 gaagaaatct ctcgtcctgt ccctgctttg cgccagcgtc ctgatgacca ccggatgcct
    26161 ctcgcccatg aaaggaagca tcatggccgt attcacggtc gatcacgtcg cctcggatcc
    26221 ggtcgtggac aacacggtcc gcccggccaa acgcggccag gccaaagcca ccggcattat
    26281 ctgttttgcc acgggtgacg cctcgatcgg cgcagccatg cggaacggcg gcatcaccaa
    26341 gatccatcat gtggactatg atgtgaagaa catcctcttc ctctacaacg aagtcaccac
    26401 catcgtgtac ggcgaataac ccttcgctaa cggaaagaga aacgggacgg ggccgcaacg
    26461 gctccgcccc gtttcttttt ctgttccgtc actcgatgtt ctggacctgt tcgcgcatcg
    26521 cctccagagc ggccttaaag gcgatcacat gccgggagat ttcggcatcg ttggctttgg
    26581 aaccggtcgt gttgatctcg cggaacagtt cctggcagag aaaatccagt gtgcggccgc
    26641 atgttccgcc cgagtccaaa gtcttggcca cctgtgcgaa atggctgccg agccgggtca
    26701 gttcctcgct cacatcacac cggtccgcga acaccgccac ttcgcgcgcc accagcgcgg
    26761 catcgaccgc cgtcccttcc tcccccaaca actcgccaag acgtttcgtc agcagcgtct
    26821 gataggtcac aggcaccatc ggcgcgcgcg cggcgatctg ctcggccgtc ttttccaata
    26881 ccgtcaaccg tttacgcaaa tcctgttcga gggccgcgcc ctcccgctgc cgcatcgtat
    26941 ccagactttc caacgccaac gttcccgctc gctccaccaa cggccacatc ttctccggct
    27001 ccagcggcgc cgcgccccgc agcaagtccg gcagacaaag cagcgaggag gcggtcaggt
    27061 catccgccaa ccccaaccgc ttggccagat cgcgcagggc cgtaaggtgc ttgccaatga
    27121 cttccgggtc ccatcccgcc gcattgccac cagcctcccc cacgctcgtc gcaaccgtca
    27181 ccaccccttt gacgtaaccg cgtgacaccc gggttcggat caacgtctgg atccgcgtgt
    27241 ccagactcga caactcgcgc ggcagggaga ggctgcaatc gaactgcttg cggttgaccg
    27301 cgctcaactc caccgtcacc ccgcgtccgt tttcctccgc ctcgccgcgg ccgaaccccg
    27361 tcatactcct gatcgccatg acacgacact cctttccgtc gggccgcgcc cgcccgtccc
    27421 ttatacccgg ccgtaccgga tcagccagtt gatcagcgtc accgcgacac tgccgaaata
    27481 cgtcggataa atcacgaaat gcagcggcat ccgcaacaca cccgcattcc ggccgaacgc
    27541 cacatgtgcc gccaacggac ctaacgcggc cagcaggatc acacccaggt ccgcccgggt
    27601 ctggggatat tcatcccaca ggttcgtgac catcacccac agaaacgctg ccgccacgac
    27661 gcccaacccg acccgccaag gccattgcat ccgctctttc aaaggcgcaa gcgcctgacg
    27721 aagaccgagc acccaggtgg cgagcacaaa gccggcccag aagacccaat gccccaacac
    27781 ctcgtccaga tagaacagcg aacgccgcac ctctggcgta agcgcgccgc ttcctgaata
    27841 cgcgctgatc atgcgattgg tcggatcatg gattcccatg ccgcaagcca ccccatacac
    27901 ggcaagcacc atcagcagat ccgccgccac gaccgtacgg ccccgactga gcgtacggcc
    27961 gatcaaccag aacacaaggc cgagggccgg cgcgaagaca aacatcgaaa gcgcctcacg
    28021 caaccgcaac acatcaccga acgtccgcaa ctgccacccc gggggttgca tccggctcgc
    28081 ccccgccgcc aagctgatca ccgcaaaaag cgcgaacagc cacgccagac gcgtcagcac
    28141 ctgacgatcc gtcaaatcat ccgacattac cgcccccttc ctcagatcct gccgcgagcc
    28201 gttccagttc gccgtaaacc gatccgagcg cggcggccaa ccgcgttgcg ctcagtgcgg
    28261 ttcggccctc ttcccatgcc gcctcatccc ctgcggcacc gtgcgcccac actgcggtgc
    28321 aggccgcctg cacgggatcc aacccctgtg cccacaatgc ggcgaccatc cccgcgagca
    28381 aatcccccat cccgcccgaa gccatgccgg gattcccggt ccggttcaac caaggccgcc
    28441 cgtccggtgc gcacaccagc gtacccgcgc ctttgagcac caccaccgcc cggtaccgtt
    28501 ccgccagcaa ccgcaccgcc gccacgcggt ccgcctccac tgccgccaca ctgagaccca
    28561 gcaaccgcgc cgcctcaccc ggatgcggcg tcagcagcag atcctgccgg gcgcgcggac
    28621 gccagccagc gtgaaacaga tcggccagag cgttgagccc gtccgcatcc agcaacaacc
    28681 ggtccggccc ggtctccaaa agttgcgcca cagtgcggcc tgcctcctcg tgccgtgtca
    28741 agccgggccc ggcgatgacc accttgcacg cgtccagccc cgaaaatcct gcggcaagcg
    28801 catgaggcga aatcaccccg cgcggcacgt ccagcgtatg acacatcgcc tcggggaccc
    28861 acgtcgcagc cacagccgcg ctctgcggcg gcacggccaa cgataccagc cccgccccgc
    28921 tccgcaccgc gccgagcgcc gcgagcaccg gcgcgcccgg cattcctgcc gcgccgccga
    28981 tcacggcgac atgaccgcac gtgcccttgt gggcgctcca ttgccgacgc ggaaagaacc
    29041 gggcgagttc cggcagagcg atgcattcac acggcgcatc atccgtaccc cggtcgcaca
    29101 acgcatccgg cagaccgata tcagccacga tcacatggcc gaagcgctcc gcgccgcacg
    29161 ggttgagcaa cccgcgtttg ggacgcgcga acgtgacggt cacatcggca tgcaccgcaa
    29221 cacctgcggc ttcgccgcta tcgccatgca cacccgaagg gatatccgca gcaacgatca
    29281 tcgcatgcgc acgctgccgg ttgatccatc cgatggcccg cgcggccgcg ccagacggcg
    29341 cgccagcaca ccccgtgccc agcactccgt ccaccaccac gccaccccat gcggaaggcg
    29401 cggcaaccgc atccgcattg tcctctgtcc atccgccgca cgcagactct gcggcctcgg
    29461 gttccgacca cggcacgccc gccgcgtcca tcgcctccca cgcctcgcgg gcaaccccgc
    29521 ggaaccgcgc gaccggagcg gtcaacagca cccgcacgag gaacccggcc tcccgcaacg
    29581 cgcgcgcggc cacacaggca tcgccaccgt tattcccgtg gccagccacc agcaccgctg
    29641 cgcgcgtccg ccgccgtgcc gccacgcgcg ccaccgcgca agccagcgcc tcgccggccc
    29701 gccgcatcaa tgcgcgttcc ggaatccccg cttcggtcac ggcatcgcga tccgcagcgc
    29761 gcatttggtc caccgtcagg aaccgcatgc cgcctccttt ttccccgcag cgtgcttcgg
    29821 cacggtaccc aggagctgct cgatccgttc cgcggcccgc cgcacaaacc gccgcttcac
    29881 ctcataccga ccgggataga acgcggcctt gaaaccagcc agcaccaagc gttggaaggc
    29941 gcgccggtca agctcgcaca cctctgccac ccgcagcagt tcggtgttga tgttcgtacg
    30001 ggaaaccagc gtgttgtccg tacccactgc caccgacatg ccgcaggcga gcatgcgccg
    30061 caccggatgc ttgcgcaggt cgccgcccaa ttcgggaatg gtttgaagat tactggtcgg
    30121 gcaaacttca atcgtcacac gcatggtcgc gatatagtcg gccaacgaat cggtgaaggc
    30181 ctgagggtcg gaaatcgacg gatcgaggat cttgtccgcc gcgaacagaa aggtgccgtg
    30241 cccgatccgt tcggcgtgac accgggcgat cgcctcatag atcgactcgg ggccgtacgc
    30301 ctcgcccgcg tgcacggtct tgcggatgaa gtgccggtgc gcctcctcat atgccgtcac
    30361 atgatggccg gcgcggtatc ccgcctcttc gccggccaga tcgaaaccgg tcaccggcac
    30421 cccctcgcgg tcgcgcgcct tcaccgccgc gcgcaccgcc tccagcgagg cgatggaaac
    30481 cagttcgttg cgcgccatgc ccggcaacac atccaacagt cggtcataat aggcacccat
    30541 accgcgctcg aaattgcgca tggcgcaaca gaggatggcc cattcgaacg ggatgtcctc
    30601 ctgcgcatgc acgcgcgctg acgcgttgtg ctcccgcgca gcccgggcca gcccgtcatt
    30661 caccgccttg aacgcttgca cacacgcctc gcccgtttcc accagccgct gcggcgcaaa
    30721 ccggacttcg agatatcgca cgccttgggc gatggcatcc tccgccatct cgtaggccgc
    30781 gcgctcgagc gcgtcacggc ggcgcaacac cgcacagagg tactcaaacc cccgcagata
    30841 atcggccaga tcgcggtaaa ccggtttgaa caccaattcg agcagtccct cctcctcata
    30901 cgagggcaac ggcacccgtt gctcgcgggc cagttcgatc agcgtcgcga tgcgcaggga
    30961 gccatccaga tgcgcatgca catcgctctt tggcaaagca cggaccagtt cgtgcggaat
    31021 catcacatat ccccctctgc ggcaggaacc agatcggggc gccactgact caggaaacag
    31081 acggcgtacc cccggaagaa aaaggtgacg ggcagcagca gcaccgcgtt gagatagggc
    31141 aggaccaacg gaatgaagcc gatgcaacag gtcaccaacc cgaaaacgag aaccgcgaac
    31201 atggcggcac tccagcaggc cataccgcag agcagatagc cggtcagcgc gaacgggtga
    31261 cggttgacga gcgcgaggac catgcgccag gcgctagcca caccacagcc gctccaatac
    31321 atcagcggca ccacgaaagc ttgggtcaga taggccacga gccggaccgc tatcagaagc
    31381 agggccagac tggtcacgca tccggttacg ggcggcaaca gcgcgggctg ccagattctg
    31441 cccgcgtcga gatacggctt gagcacatga gtgtacgcga tcgccgcatg caggacgaac
    31501 agcgccgccg caaccagaaa gaacaggacg cgccaaccga acaacgccca tccgctagac
    31561 cgcgaagcca tccaacacgc gtgcaccggc gtatccggct tgtaccagcg gtgcagaaac
    31621 atgaagtccc cgcgcgaccg cacccagcac atccaaagcg cgaacagcag gccgagcaga
    31681 atggcgaacc ctgtccacgc gagtttcacg ggctgttccg gcaacagctg caggacgtca
    31741 atcatccggt cgacctgttg cttgaatcct ccggccgagg tctcgcccaa ctgctgcaga
    31801 ttgaccgtgg actgcgtgcc cagataagcc agccacgcgc agaaaccgat gctgaaccag
    31861 cgcacgggat cgaacggccg gaaaagcaga gccgtcatgc gcgcgaaggc ctgcgatgcg
    31921 gcccgcaacg cgtggggccg acccaacgtc acctccccca tgccggatat cggcggtgcc
    31981 gccgcttccc tgtacggcac ctcgaacatt tcgcgcacct ggcccgcatt acgccattcc
    32041 gggccgaatt cggggtgcca caccagatcg tactccgaga tcctcccggt ttgcgccagc
    32101 ctgcgcaact ccgtgaaggt caccggaccg aaccgctcac cctccaccgc gtaataccac
    32161 tcgttccgca tcggctcatc ctttccgcat cacccgcaac acaacgatcc ggtcaactcg
    32221 ccgaccgaac gcatcccgtg ccgacggagg taggactcca atccgtcgag cacgcgcaac
    32281 ggggtcgagg ggtcgatgaa attagcggtg ccgaccgcca ccgcgctcgc gcccgcgatc
    32341 agaaactcta tcgcctgctc cgcacccatg atgccgccca tggccacgat cggcactgcg
    32401 gcgacctgcg cggcctccca caccagtttg actgcgaccg gatggatggc cgggccggaa
    32461 aggccgccca cccggttggc aagcaccggc ttgcgcgttt caatatcaat caccatggcg
    32521 ggcagggtgt tgatcatcgc cagcgcatcg gcccccgcct cctgcgccgc gcgcacgaac
    32581 acccggatgt ccggcacgtt cggcgccagt ttcggaatca ggggcagccg ggtcgcccgg
    32641 cgcaccgcgc tcaccagaga ggataccgcg cacggatccg tgccgaacgt attcccgccc
    32701 tccttcacat tcgggcagga gatgttgagt tcaagtccgg cgacgccctc gacctcggtc
    32761 agacggcggg cgacctccgc gtactcctcc acgctcgtgc cccagatgtt gacgatcacc
    32821 ggcacgccgg tcgtgcgcag aaacggcata tactcgctca cgaacccgtc cacgccgggc
    32881 ccctgcaatc cgatcgcgtt caccagaccg cccggcacct ccacatggcg cggcaacgca
    32941 ttgcccggcc aaggctggag ccggatccct ttgacggtga tcgcgccgag ccgggtcaca
    33001 tcgatcagcc cggcatactc cctgccgtat ccgaacgtgc cagaagccac tgtcaccgga
    33061 ttttgcatcc ggatcccgcc gagattgaca cgcaaatcca aatcccgcat tcctgcccca
    33121 acttccccgg tcatgaccag atgatctccc gcgcctcaaa gatcggcccg tcatgacaga
    33181 cgcgtccgat ccactccgaa ccgtcaggcc gccgcagtga ctgcacgcat gccagacagg
    33241 cccccacccc gcagaccatg cgcttgtcca gcgagagcca ggcgcggcat ccggtctgaa
    33301 tcgcgcgatc acccaccgcg cgcagcatac cgtccggtcc gcatgcaaac agctccaccc
    33361 gcgcctccgc accgcgcatc gtctccaact cactgtccaa caggtccgtc accagaccct
    33421 gctcgccgag tgaaccgtcc tgcgtggcca cccgcacccg ccaccccaac tccgtgaagg
    33481 tctcgatggc caacacatcc gccgcgcccc gcccgccgac gagcaccaca ccccggaccg
    33541 gcaaccggct cgccagaaag gcgagcggtg ccacaccgta ccccccggcc accagcaccg
    33601 gcgtaccctg cggctccagc gggaacccat tacccaacgg accgatgacc tgcatccgct
    33661 cccccggttc gacgcggctc aactgttccg tgccgcgtcc caccgttttg taaagcaacc
    33721 gcaacacacc cccctccgcg ccgtacaggc tgaagggacg gcgtaacgcc gaacgctcca
    33781 gattcggcac gcgcacatgc acaaactgtc cgggccgcgc ctcggccgcc acctcgggtg
    33841 cctccatttc catcacccag gctccggccg tcaacggaat ctgacggcgc acccgacccc
    33901 acacatccac cataccggct tcccctttcc cgcatccgcc gaggccggac gcgctcatcc
    33961 gtttccgcct gcggcgggct cggtcttcgg ccacacagcc gggggcacgg gcggcccctg
    34021 cggcggcgac aactcgttct gcgcctcgat gtaatccacg cgcgtgcgga cccgtttgat
    34081 ctcggcattc accgccgccg ccgtctccat ctgaccgccc accgtgagtt tcttctgcac
    34141 atcctgaagt tttttcacat acttttccgt ggtgtccaca agttgctcgg cccgtttgcg
    34201 ccgattctcc tcgcgcagca ggaaaaattt cttctgcagt tcgaccagtc ccgcaggctg
    34261 caacacaaaa tggcgctgct ggatcgcgcg gtcggcctca aaacgcccga gctcgtcgcg
    34321 cgcgctctcc caccccacat aatcgcccgc tcgctggaac gattccatga gttccgtcag
    34381 cccctgcaca tattgatcgg gccaggcggt ggctttgcgc acggcatcct tttccaccgc
    34441 cgccagctca ctctcgaacc cgctccggag ctgcgcgatc tcctccacgc ggtgctggtc
    34501 cgaaccgccc agcagcatcg gagccgtttc ggtctcggac atgcccgcca actcggccgc
    34561 cgccaccacc gcttccgcag ccagagccgg aggcgcgttg cgcgccttgc ggatttcggc
    34621 gttcaccgca gaggcgagat ccatgcgccc ttcctgcata aacttccgct gcatatccgt
    34681 caggtcgttg acatattttt tgcagagcgt cacccatttc cggctgtgct gcaaccgctg
    34741 ttccgaaagc atctggcggt acttggtttt cagcgtgttc agttcggaca acccctcgac
    34801 aacggcgtcg ttcatctgcc gtgtctcatc gaaccgcctg cgttcggttt gcgccacatc
    34861 ccacccctcg aagtctcccg ccgtgcggca ccgttccatc aagagatcca actcgcgcac
    34921 gtattcggtt ggccacgccc gctggaactt ctcctgccgg gcatcgagtt ccgccagttg
    34981 gcgcgcgtac tcgcgctgct tctgcgccag cgcgtccggc tggggcgtgg gctggacctc
    35041 cccgggaagg acgggctcct ccgaagcggt tgcgaaatac acgttccatt ccggcggggc
    35101 accgagccag agtcgggcgt aggcctgtaa cggcaccctc gccaggctgc gctcatacac
    35161 ccaccgggcc ggccgataga aaccgcgcgc cagcgtgccg aaggaccggt cggccagcgt
    35221 cacggccacc ggcaggctca gcagatacaa caccatcatc ccgagggtga tcgcgcaggc
    35281 gatccgcaga ttggccgctg cggtatgtat ccggatcgcc ttccgaatcc gcagcacgtc
    35341 cggatgatcg ggcgcgacct cgcgcagcaa cgtcccgcag agacgcgccg cggaatccca
    35401 ctcgcgctgc ttcagcgcac gttgcgcgcg ctgtagatca cgcagcagaa tgcgctcggg
    35461 tatctgccgc agttcgtttt cgaacccctg gcggtcctct gtcaactcgc ggtactgccg
    35521 gatgaattct tcggcgcgct caaaattctc ggcgcggatc tcgatcggga tctgctcttt
    35581 caattgatcg cgccgacgca tttttttctc tgccagagca cgcagttcgg tcacttcctg
    35641 cagataggcg cggccggagg gtccagccgg ttcgaaccca tgcacacgtc cggcatgggc
    35701 gatgacacga tcgaaacgct gctgcgtatc cgaatcgcgc attttctgca gcagatggag
    35761 caccgattcg tacgcccgcg cgtccgcacc gcatgtgccg caatattgga cgcccacaaa
    35821 cgtgtcggct ccacactcgg gacaagcttc cgacccgtcc cacccgcact cggcgcagaa
    35881 tttgatcgcc agcggattga cggcgtcgca ccatttgcag cgccaggtgg ttttgaccgt
    35941 aggcgtctcg atctgcgtct tggactgtat ctggtacagc gccgtattga aggccgcggc
    36001 cgattcccac cgctgttcct tatcggtggc gagtgctttg accaccacat cccgcagcga
    36061 cacgggaatg tcctgttcgc gaaagtagcg cgggttctga ccggtcagga cgaagtagag
    36121 caacgcgccc aacccgtaca cgtcggcacg ctcgtccgat tggctcgcgt cggactcctg
    36181 ttcaggcgcg ccgtaaccta acgacagcag cttctcgccg gggaccgtga gtttggcctc
    36241 ttctttgctc atgaggcgcg ccaacccgaa atcaacgatc ttgggctcgc cgctcttgtc
    36301 catgaggatg ttgctgggct tgaggtcgcg gtgaatcacg ccgctggcat gcgcgtacgc
    36361 cacggcgcgt ccgattttca gcaccagttc gatcgcctcg tcaaccgaca actgcccgtg
    36421 ctgcgacaca tactggtcaa gggtcagcgg cggattgggt tgcagcagtc cgcccggagg
    36481 catctcactc accacgtccg gccgatccgg accggcgatg tattccatca cgatgaacgg
    36541 gccgtcctca tcctcgccca gcgcgtagat gtgcacgata tggacgtggc tcaacaccgc
    36601 caccgcgcgg gcctcgtgca gaaatcgctg gcgcagcgtc gggatggact gggcctgatg
    36661 gttcagccgt ttgattgcaa cgaagcgccc cagccggcgg tcccgcgcca gatacacgac
    36721 acccatgccg ccgttgccga ttttgctgta gatcgtgtat tggtcaaaaa gcttgtcgtt
    36781 gccgggctcc tcatagtggg atgttccctc accgggcaca acgcgtaccg tctgcgtctc
    36841 gtcttgcgcg ggcgcggcca gacgctcggt cggcggaacc tgaattcggg ctgtgttttc
    36901 cggctgcaca aatctctcca atcgtatccg cgttccgggg gccggtcaaa aacagcccac
    36961 atcggcggat cggattataa gtatacggcc ttgcggtccc atgcgtcaat tcgcgtgtgc
    37021 ccgctcgaaa acgataaagc aggcgtcatg agggcccaga aacaacggcg ccgtcacccg
    37081 tccgtcaccc gtccttgacg cgccctgcac caccagatcg gtctcgccgc tcaccgggtc
    37141 ccagcgcaac ggcattcccg cccccgcgtc acggaaggtg cagcgtaccg gaccggcctc
    37201 cggcgtgtca ttcaccaaaa agtagatttc ccgttccggc gttcggcggt gcaggaaaga
    37261 gaggcgcatg ccggcttgtt cgctttgcca gaacaggtcg ggccgcggcg cgtccggcgc
    37321 accgggccgc agtcgcctgc accctgcggg catcgtctcc gccgcagttg tccccgccgc
    37381 gccgtcggcc tcttccatca gccacacggt caccccacgc gccgcgatct gcccgatccg
    37441 ccgtcgcgcc gcctcccggc ccatcacgcc ggccgggacc accagcgcgt tatacgtgcg
    37501 atccgattca aagcggatcc ggccctgact gaccgtggcc cgttccatca aagccggtgt
    37561 ggcgtagtcg caggcatatc cctccaagac cggcgggaca ccttccgtcc agaccagaaa
    37621 atcggcaacc ggctcgccgt gctgcaacaa cacctggcac cgccgcaccg tgtcgcatcc
    37681 ttcccgcagc gagcggaacg cggcgtcttc atcaggcaaa tcccctgaag gcaccagcaa
    37741 caaccgtgtg gccccttcca ccagaaggcg ctccgcctcc ggtttccatc ggaagggcag
    37801 cagggttgcg gcaggcgttt ccgccagatc gttcagcggc agacgtccga tcacgaaacg
    37861 ccgcctgagc acgcgcgcca cccctgccgc acggcggttc acgccagccg catccgcgtc
    37921 ccgcgaaacc gcaaacatcg ggcgtcggaa gctcaatggc acctcctccg gctcgaccgg
    37981 cacagcgtcg atcgcgatcc ccgcctcgag tcccgcctca tgcaccaact catcggcgtg
    38041 ccgggcgaac ccttcgcgcc acgcctccct gacgctctgc ctgagttgcc gccggacata
    38101 ttgcgcggtc gcctccccgc ccaccggcac acctgccagc acgggcagga aacgcatgag
    38161 gttcaggccg ctattccgca ggaaacgctc cggcaaatca gcgggccaca ccatgtcatc
    38221 gcgttgcggc ccgttgaact ggtaccagag cagcgtggtc ccgtacgtct gcggcaaccg
    38281 gctctggcag gctgaaagcg tccgattcac atggcgggca aaactgttgc ccgcataccc
    38341 gtcggtgagg ggcggcgtca cttcccgcaa cgcgaaacgg aagacacgcc acaccccggt
    38401 tgtcggaagc gcggcgcgcg tcagatccac catctcgtgc ggatgcaccg ctccgctccg
    38461 atccgcaggc acagcccaga ccgccaacgc gccgttctcc tgtacgacgc gattcagcgc
    38521 attcgttcct ccgaatgccg tggacggctc ttcggtccag accaggcacc gcgcacggtt
    38581 cttgccgccg tccccggcga acggaaaatc gtgcaacccc aattcgagac ctagcctgtg
    38641 gcaggtgtcc gcaagccttt cgacgatcgg ccacaccccc tcctccggcc cctccacagc
    38701 cgccagcaca ctgccgaacc cgagcgcctg cgcacgttcg agttgccgga cgacctccgc
    38761 ttgattcgtg tgcgcggtgc ggaaggtcag ctccaccccg ggccgcgtcc aattctctcg
    38821 cggcgtgcgg aaccgcaagg ccagatccga accgcacacc ggcaacgcga acccgagcag
    38881 aatccccagc acgcctcccg cgatccccac tgtgtaaagt gaaaccataa attgagcata
    38941 ccatacccgg atgtccccga ccaaccggat tacggtgaga ttgaacctaa aaagagcagt
    39001 tgaaccttca actctctttg gtttcaaggt ttcggggttt cagggtttcg gggtttcagg
    39061 gtttcggggt ttcagggttt cg
//
LOCUS       DAOSLE010000011        37229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1276_length_37229_cov_11.751627, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000011 DAOSLE010000000
VERSION     DAOSLE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 37229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37229
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1276_length_37229_cov_11.751627"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK576_01695"
     CDS             complement(<1..403)
                     /locus_tag="PK576_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60335.1"
                     /translation="MKQISWMLAGLVLVAGCAQRPWGQRGALAAPQALTVDYLQDPVG
                     LDHPAPRLSWKLTAARRSVRGLRQTAYQILVASSPERLRDNQGDLWDSGRVASDQSLN
                     VVYAGKPLTTSQRCYWKVRAWDNQSDTPSAWS"
     gene            complement(480..1250)
                     /locus_tag="PK576_01700"
     CDS             complement(480..1250)
                     /locus_tag="PK576_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60336.1"
                     /translation="MIHLEHIHATYNGQPVLTDVSLHLERGECAALVGPNGAGKSSLL
                     RVITGQLPSQGSRRIAGGDPARCNAREFARHVAVVPQELPQDISFAAYEFVLLGRTAY
                     LPRFGSPSKADHDAVQEAMNVTGTWHLRHRALTAMSGGERQRLAIAVALAAQPEILLL
                     DEPTSHLDLRHRVDLMALLLRLNRDGKITILMVVHDLTLASQFFSRIILLNQGVKLAD
                     GTPAEVLRPDLLETAYGCPINVIPLPDFAAACVLPVRA"
     gene            complement(1277..2254)
                     /locus_tag="PK576_01705"
     CDS             complement(1277..2254)
                     /locus_tag="PK576_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE60337.1"
                     /translation="MTPSRTTTCLLLLTVPVVLILCLLAGPAGFGLPDTSTPAGRALL
                     ELRLLRVVTGFVVGAALALSGCALQAILRNALAEPYVLGVSSGSSLGAALMIVTGLAQ
                     LTPLALPAGAFVVGGLTLLLVCLMSGRAGHYSPNTLILVGVVASSMLSSLLMFVIALA
                     DTLALHSITWWMMGNLQATSWTLVLIVTVLIAISAALLFSQTPALNALLLGSETARHL
                     GVETRIVLPLVLGAAALATSAAVSLAGCIGFVGLIVPHTLRRVVGANHRRLLPATTLC
                     GGAFLVVCDTLARLLFAPREIPVGVITALSGGPFFLHLLLRNHETHETP"
     gene            complement(2251..3129)
                     /locus_tag="PK576_01710"
     CDS             complement(2251..3129)
                     /locus_tag="PK576_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOE60338.1"
                     /translation="MIVCGLSLSLLTACIRQDPLPADSGMRLVSTAPHLTECVCAIGA
                     GHLLVGRTATCDHPPEVVSEIPVTGDFAVPWLEPLLAVRPTHVLETVLSDPALRDRLT
                     ALNIPVVHVPCTRLDQIPDALRQLGVLCDRSTRANQLADEIQSGIESARQAASSCQAR
                     PRVLLLFAPDVPITAGRNAFVTTLLELAGGVSAGHDLETNYLRVSLEWIIEQDPDLLL
                     CIFATARPPLEIYAGQTGWRSLRAVRSGRVYTVTDLNTVSRPGPRVLDGIAQIKAVLD
                     QDAERFAKQSPTPDRP"
     gene            complement(3395..5425)
                     /locus_tag="PK576_01715"
     CDS             complement(3395..5425)
                     /locus_tag="PK576_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE60339.1"
                     /translation="MSLSHRFTAATFAACLAATGLTTLGQTTPEAPESSPTAPSVSNA
                     IPPIIVQASRTGKTSLQMPASVQVITAREIAESGCSSTAEVLEKQGGVTLRTLNSNPA
                     LTQLSMRGFGESSFGRVLVLVNGERLNNPDMSSPSLLRVPMASISRIEIIRGSQTVLH
                     GDFASAGVINIITDDATAEQQTTLGASVGAYDTYSAFVNTTGMFGEDGVSYRASADWE
                     KSDGYRDNSDYETYQLNAALRKDFGEDRFLSLSTFYHDAEYGLPGTLSRERYHNAPRS
                     STTPRDRSELESYGIRLGGRTTVGADGIFDLNATASRRETDSRWLSATSQAFYGSEID
                     SYALQPQYTHDLRIAGHRSILTLGTDLRYDVTDFYSDFNSPFYTSLLDWEFNRASIAG
                     YAQNEFFFTDDLSLTLGGRTEWFDNRVKNAASTTSYSKRESAVEAALLYRPTEASKVY
                     AKAGRFYHAPFIDEIFAGAGTPNLALTPETGLNLEVGAEARIANEWLAALSLYHMDMD
                     DEIYYDPATFQNRNSPGDTDRIGLDAALHWSRDRVGSATLAYSAVRAEFDGGPYDDNR
                     VPLVPTHTLSVNGDYYLLHSLALMGGVRYVTRQYLGSDFNNSAPPLKGYTLVDCGLRY
                     EPSFIEGLRVTLSVDNVFGRDYCDYAGWSSFSGDYYYPGRGRFWKVGATFTF"
     regulatory      complement(5547..5709)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6292..6693
                     /locus_tag="PK576_01720"
     CDS             6292..6693
                     /locus_tag="PK576_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60340.1"
                     /translation="MRQGLRVAFFCLAVTVGGGMAGFGQDFSMLSEAERKQVNGWMAE
                     RAAILADAHKIEIELDQAWGNPQFTSPEVEAQRERYRQLQHELMMTQLTLRRLVEKLP
                     AAQSRQSDLDALRNRAEALSRQIEAKGGVKR"
     gene            6740..8281
                     /locus_tag="PK576_01725"
     CDS             6740..8281
                     /locus_tag="PK576_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOE60341.1"
                     /translation="MTRENKSSTGKNRRPRILVVTPEITYLPSGMGNMACKLNAKAGG
                     LADVSASLVAALFDLGADVHVALPHYRRMFHIDVGQLISDELRVYKSRMPDSRIHLAE
                     DRCFYYRDTVYSNSQDENPKLALAFTREVLNNIIPFVQPDLIHCNDWMTGLVPGAARR
                     MGIPCLFTVHNIHTHHLTLAQVEDSGIDAAEFWANLFYTRVPYNYEESRNTNQIDMQA
                     SGVFASHYINTVSPTFLEEIVNGWHDFIPTALREEIRNKHRAGCAIGILNAPDASDNP
                     ETDPHIEVCYDAATHREAKRANKVAFQHKVGLEEDPDVPLFFWPSRLDPAQKGPQLLA
                     DILYRFMSRHWRQRVQIALVANGVFQKPFHDILHFHEMYGRLAVCDFNQGLSQLGYAA
                     SDFTLVPSLFEPCGLPQMVGQLYGSLPVVHDTGGLHDTVEHLDPAQNQGNGFVFRIYD
                     SRGLDWAMEKAIEFYKCAPEVRERNIARVMREGKARFNHETCARAYIEIYEKMLNRPL
                     VRSFE"
     gene            8317..10329
                     /locus_tag="PK576_01730"
     CDS             8317..10329
                     /locus_tag="PK576_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOE60342.1"
                     /translation="MPATLRRPRLLDDPYLLPYAEIIRRRAELADRREADLSQGPGKL
                     AEFACAHEYYGLHRTDSGWVFREWAPNATALWLVGDFSSWQCQEPFRLQRIPGRGVWE
                     AHLAPDAVRHGQYYRLEMVWDGGRGERIPAYARRVVQNPETLVFAAQVWDPEPYVWRN
                     PDFRVPDRVPLIYEAHVGMAQEREGVGGYTEFRERILPRIKRAGYNTVQLMAVMEHPY
                     YGSFGYHVSSYFACSSRFGTPEELKALVDAAHGEGLAVIMDIVHSHAVKNEREGLSRF
                     DGTLYQYFHDGLRGWHEAWDSRCFDYGKTDVLHFLLSNCRYWLDEFRFDGFRFDGVTS
                     MLYLHHGLGVDFMDYSQYFDATVDEDAWIYCRLANRVIHQVRPDAMTVAEDVSGMPGL
                     AAPLEDDGAGFDYRMAMGVPECWFRLVRDVRDEDWSIGYLWHELTNRRADERTVNYVE
                     SHDQALVGGKTLFFQLVDAAIYDAMLRSMRHLEVERGVALHKLARLATLATAGHGYLN
                     FMGNEFGHPEWIDFPREGNGFSYRYARRQWTLRDNPELLYWCLGEFDAAMLEMVTRYR
                     ALEGTLPRRLFVSDQEKILVFERGPLVFLFNFHTHTSVADFKVLVPPGSYHWVMDSDE
                     ERFGGQGRIAAGQSFALIAEQRDRELCQTLRVYLPCRTAMVLERRI"
     gene            complement(10346..11194)
                     /locus_tag="PK576_01735"
     CDS             complement(10346..11194)
                     /locus_tag="PK576_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE60343.1"
                     /translation="MLRSSYHLCTVFGIPIRLDASLIILMFLLIGNYGNPLYGIAAGI
                     ALLVSITLHELGHSLTAMAFGCRVRDITLMMMGGCATLLSMPRKAWQELLMAAAGPAV
                     SLGLALAGLLLPDVTYRDGHVLFASGWDRFLFLQIGALNAMLFVFNLLPAFPMDGGRM
                     LRAILQQFFMTRLRATWVASRIGRFLALLLGLSVLYSLFTGRFDGFKLIRLLIAYFIY
                     QAAEGEYRMVLAEERAGRATSPFDGFPFMGGRSSAMPPPDDGKAVVSPPPYERRGATR
                     VDVHKT"
     gene            complement(11209..12195)
                     /locus_tag="PK576_01740"
     CDS             complement(11209..12195)
                     /locus_tag="PK576_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOE60344.1"
                     /translation="MNPSLSHLVFQPVYKRYVWGGDRFRTFFHRTGVPAVCAESWEIS
                     AHPDGMSVVSCGPFAGQTLAELTARYGAALTGTRAPDPARFPLLFKLLDASDRLSVQV
                     HPNAACAARTGGEPKTEAWVVLDCTPGASLYAGFEPQVTPEVLRAALSDGTVAEQLLR
                     FEPQPGQALFLPGGMAHAMGAGCLIYEVQQSANTTYRLFDWNRTGPDGRARPLHIGPG
                     LASIDWSLPPPQLLTPQLPCPTGTAPVWSEIVTSPVFTVRRLDLREPLRVAPDGTTFL
                     ALFAAAGRVTVADAGNRQHATLPPGSSLLLPATADAHILTPHPESTLYVTTL"
     gene            12446..15739
                     /locus_tag="PK576_01745"
     CDS             12446..15739
                     /locus_tag="PK576_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60345.1"
                     /translation="MRHLPALLAVFCLAVLCLRAGEGEWQRGFLGKAEFNGTLKGSSA
                     ETEWELVFTRKGRALDATGGVHEVNQPIFNREGLRMAGGRETEYSDSSGAITNHAGIF
                     QTREILSLPPVLTQRMPEDDFTVLVTFSVFKTGGLYQTVFEQTRRQRWWPTLHLGFRS
                     QHRNQLTLHHADRTDSSAKRFAETGVLPETGRVYTVVLTRQRGTAKVWVDGVKRAELA
                     GIPVEDVSDWNLGCGGPERALDGHLRTFAAWDRALDEEEVAAIDLNDLATAGLVRVTP
                     LRYIRHLARGFEPRSYSFQVRNLSDTALEGVAFEWEIGDQRFTQPVGRLEAQGERVID
                     VPVLITLKPGDTVQTFSVTAGGKALNRQSLPATVTPAPVPVNQLQTSSWCQFLPGEPG
                     YTMATVRHCADAMLAGLHWGPVQLRWTGWARADHPEDRAFTPHQKRSYNDFYFSERMR
                     EQMRVCTDGIVRRLAFYPALTHIDINNEYNPNFQVNFSEPARRYAAEHFGLDLTPWMG
                     DGKRELVRPIGRLSPVLANYPIPASRIIPASDPLYRWHLDRKGPHGDSESLLNQHIAT
                     AMRRERPDVLSVVAPLTRRLSLRGYDDRLDIAQTWCYTPGLRVVWLQEALTAHIRHQP
                     RMRSSSFPAFLHMNAGPYPAMPSGDIFAASCWASLIRPTSMLVLFQALEMMRGHGLPK
                     AEIDALLGSTAPNAEEAARVIRAKRLSFKGDHPTLAPRVRRLLAEELPPFRALLPAWS
                     NAPRQIAVYSCFADSLFADQRWPTDYTPLLAELTRSGVAYDLLFDEDFTRDPKLLDGY
                     DMVFIPNLTYLFEPAWQALRTFVETPGRVLAVPDTAHVFFPNAVTVRDTGGALSAEEH
                     ARIVAEVESRYGADSGRPAVIEALDARLAEAAAQGADAGPFAPLLDRLGRLNARCLMP
                     HTALNVTRLDDTHFLSVVNDLRTWGPIYGHFRVLPERGVRQTGDVLFRSALGRFAYNL
                     RTARRLAVTTGGNGQSRLSLTLDGGDAAILLLSDHELGRFRAGIVREGTYMRVTAQAC
                     DTTGREIDVSMPCRVAVEGIPDYSGVALFRKGKLDYRFPFDGTFPTVTLREEATGATV
                     TLRTATLRD"
     gene            15866..17155
                     /locus_tag="PK576_01750"
     CDS             15866..17155
                     /locus_tag="PK576_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60346.1"
                     /translation="MKRMLIGTAFACALAGMGMAAGVLEEGFVSLFNGKDLSGWTGAT
                     GGYAVENGVLFCKEKSGGKLLTEKQYDNFILRFEFLVQTNSNNGLGIRCPAEGDAAYN
                     GMELQILDDSGPKYTALKPYQYHGSIYGVVPAKRGHQKKVGMWNFQEVRAIGSRITVI
                     LNGEVIVDADLSTINETMDGKPHPGLRNTKGHIAWLGHGSRVDWRHIRIKEVPADYTV
                     ARPTDNRAPEGFTLLFNGKDMTNWKGVTREERFDNPLVRQQATPEKRAAMQKKADELA
                     RKHWHIRDQALYFDGKGYSLATAKDYGDFEMWVDWRLLSVRGDSGLYLRGSPQVQIWD
                     AHNQWNIGSGGLYNNKKNPSKALKIADRLPGEWNTFYIKMVGERVTVKLNGELVVDNT
                     VLENFWDRSKPVFPKEQIELQCHGDPLEFKNIYIREL"
     gene            complement(17233..18408)
                     /gene="nspC"
                     /locus_tag="PK576_01755"
     CDS             complement(17233..18408)
                     /gene="nspC"
                     /locus_tag="PK576_01755"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOE60347.1"
                     /translation="MPLPTPAFVIDTAALERNLAVLREVKRRTDCRILLALKAFSMFS
                     VFPLLRRTLDGCCASSVHETRLGREQFGGEVHAFAAAFSEGEMSEIATLADCVTLNSL
                     SQWRHFRHILVSVGRDIPCGLRINPEHSEGTTPLYDPCAPGSRLGIRRETLNNERLDG
                     ITGLHSHTLCEQDAAPFQRTLEVIEARFGDLLPQMRWMNFGGGHLITQPGYDLDLLCN
                     TLNAFRERHPQIETLYLEPGAACVLNAGTLAATVLDVVENDMPIAILDASCACHMPDV
                     LEMPYRPRVFRDAGWHGEAVPGPQADCGADPGVKAHTVRLAGPTCLAGDIIGDWSFDS
                     PVRPGDRILFEDMAHYTMVKTNTFNGIRLPTLAVREPDGAIRIARVFGYEDFKSRLS"
     gene            complement(18506..19354)
                     /locus_tag="PK576_01760"
     CDS             complement(18506..19354)
                     /locus_tag="PK576_01760"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOE60348.1"
                     /translation="MNTPLFEDAFRALPPPCTDGEPDCGMILGSGWSQTLSVDPPIAE
                     IAYDQIPGLGASTVVGHAGRFVLFERHGIRVAAFLGRRHWYEGAGWEPVVLPVELLRR
                     LGTRNLLITNAAGGIRPTFKPGDLMLIRDHINSVGLNPLIGPVLPGWGPRFPDQSRVY
                     DPELTNLLREAAHDSGTSLSDGVYVFTSGPGYETPAEIRAYSGMGGDAVGMSTVPEAI
                     VANAAGMRIAALSCITNMAAGISGPHLSHEEVLEQTRVTAPLMSSLLDAFLARLAHQI
                     RRVPRE"
     gene            complement(19351..20718)
                     /locus_tag="PK576_01765"
     CDS             complement(19351..20718)
                     /locus_tag="PK576_01765"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOE60349.1"
                     /translation="MTFPVFHGTNTEVRSKLLEARLTVRCAPVYLSGMVNLILERVRV
                     IDPAEGRDETADLVLRDGILQGGAATTEGLRRIRAEGWIAAPGFWDVHVHFREPGNTQ
                     AETRRSGAGAAAAGGFTHVVTMPNTSPAGDSAVWVQEQAADDVPVRILPAACVTRGRQ
                     GRDLAELEALAQAGAVAFTDDGAMVADEDVMRCAMTRARALNRPVMDHAVVPSIAGKG
                     VVRDCPLARANGWPIFPAEAEVEAVRRDIRLCRETGCATHIQHVSCAESVAAIRVARA
                     EGLPVTAEVTPHHLALAAEDIPSDDGHFRMNPPLGTRKDARALREAVLDGTLGLFATD
                     HAPHTPETKTHGFLRAAFGVIGLETAIGVTWRILVEEEGMTPLDWVARWSCGPAALLR
                     QPVPALRPGLPVDLVLIDPATPWIVNPETFRSRSRNTPFAGWNLHARARLTVCGGRAT
                     YSDLP"
     gene            20852..22156
                     /locus_tag="PK576_01770"
     CDS             20852..22156
                     /locus_tag="PK576_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002945316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOE60350.1"
                     /translation="MSKDSVTRETVLAFHIGGKIGMRMSKPLQTQTDLCLAYTPGVAH
                     AVREIATHSAKVFDYTAKRSLVAVVSDGTAILGLGDLGAEASIPVMEGKAVLFKSFGD
                     VDAWPVPLNHCRQGGANTGKTDPQRVIDAVLAIAPMYGGINLEDIAAPACFEIEDRLD
                     ELLDIPVFHDDQWGTAVITLSGLMNYALLSDRSMESLRVVINGAGAAGIRIADMCKAA
                     GVQDLVMCDTRGVIHTGRADLNAHKRRHACDSAARTLQEVLRGAHVFIGVSAADAVTA
                     EDVRGMAEYPAIFAMANPDPEIRPEGVAEVLGDKPYVMATGRSDYPNQVNNVLGFPFL
                     FRGALDVKARTINTAMKVAASEALAALARQPTPSEVQALYPRENLVFGNGYIIPKPFD
                     RRLFTGVPLAVARAAVASGAAPADTDLEALQVELAARDAARG"
     gene            complement(22222..23028)
                     /locus_tag="PK576_01775"
     CDS             complement(22222..23028)
                     /locus_tag="PK576_01775"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOE60351.1"
                     /translation="MKPLQIGGRLFRSRLFIGTGKFGSHDVMRQAIEASGAELVTVAL
                     RRVDLDKNPAGDPLLSQLDPEKVLIVPNTSGARTAEEAVRIARLARAAGGYDWIKLEL
                     TPNLHHLLPDPIETLKAAEILVREGFRVLPYINADPVLAKRLEEVGTVAVMPLAAPIG
                     TNLGLRTRDMIEIIVSHAQVPVVVDAGLGLPSHAAAAIELGADCVLVNTAIATAADPV
                     AMAQAFALAVRAAEAGLDAGPRAARLDAEASSPLTDFLWQEPEATPDNVR"
     gene            complement(23116..23316)
                     /gene="thiS"
                     /locus_tag="PK576_01780"
     CDS             complement(23116..23316)
                     /gene="thiS"
                     /locus_tag="PK576_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOE60352.1"
                     /translation="MTVTVNGKPRPLGKAHSLADLLKALGVNAIHMAVERNDVIIPAH
                     AFETTPVAPGDRIEIIQFVGGG"
     gene            complement(23313..24383)
                     /gene="hydE"
                     /locus_tag="PK576_01785"
     CDS             complement(23313..24383)
                     /gene="hydE"
                     /locus_tag="PK576_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOE60353.1"
                     /translation="MVSLRHLKKMHQGALPSVAELASLLKAQGRVRERIYRAADTLRR
                     ENMGDAVFLRGIIEFSNVCKNNCLYCGIRCGNPHVGRYRMSADEIVNTARQAKHWGCG
                     TVVLQSGEDPFYTTEILCGMIQRIKEETGVAITLSIGVRPRAELARLRTAGCDRFLLR
                     FETTSPALFAAIHPDESFEHRVQCLTDLRDLGYQVGSGFMIGLPGSTPEMIAQDILFA
                     TQLKLDMIGCGPFLSHPGTPLAEKPLLEDRSVYYKTVALLRLLNPQAHIPATTAFDAL
                     EPEGRNGVLMRGANVFMPNATPDKYRRMYQLYPGKPYADEEGATCAMYARERIAAIGR
                     EVDSGPGHARRLTVSADRKECT"
     gene            complement(24528..27212)
                     /locus_tag="PK576_01790"
     CDS             complement(24528..27212)
                     /locus_tag="PK576_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60354.1"
                     /translation="MRFTGKMTIAVAAVVAGTAAAAEFTIATNGSDRAEGTAAAPFAT
                     FSRARDAVRALRMREPGRAVPVVVEIREGVYELEEPLAFRTQDAGTPEAPLLFMAADG
                     AKPVLSAGRRITGWKTDADGVWRATAPGTARRERAFSQFFVNGRRRLRPAFPENGYFV
                     PEANLPGDTNTRDSRKGFVFARGDIQPSWHHFRDVEVSMIWAWQYARAHVSSIDAAKR
                     EAVLSLDADKLFNSSIRTDIYYRLVNIREGLKRPGQWYHDRETGEIAYIPEQGETPDE
                     AETIASCLENAVVIRGEKGGKHVSDITFRGLTFAYTDWSISTNGYWDSQGAAELPAAI
                     HVDYADRIRFENCAMRNTGGTAVRFGLGCRDCAADGCEMTDIGGGGVASGAFWKGSGD
                     KDNETSGTVVRQCLIGWYGRDQPGAVGILIGGANRCLIAHNTVHDGYYSGISVGWTWA
                     PYPSFTRENTVEWNHIYGYAPGVLSDGGGVYTLGLQPGTVIRHNRVHGAVAARYGFHS
                     FYFDQGSSCLTLSNNLAYDTDSGVVIPSEDKSGNLIVNNIFADGRDQQFYANDRTADC
                     KFGTNRIERNLFVWRGANRGGNTGAPNRYASNLWWQVDSPERIQTAFNGKTFAEWAKT
                     DTGAVFADPQFRNADMGDFTLSPDSPAHALGFVPFDLTDAGRTLPLCFAGDQPLPGET
                     FPVAPLDQHKTLSTGFEECCAGTTKFGLAGWHTEGRNPSNLLVFVTEKNAAKGRHALK
                     MLNDKSIGPTPALRSWGRRLSGRQTHQFDMYLEPGATFEYSILDANVPRRRYAVETAV
                     FRVNGQGDLAGADGKTVMRLPSGTWVHVEMRISLGLAACGAYDLRVCLPHGNSRLVTA
                     IPLPRTLRQIDGFRFAVGGQDKTAVCLDNLDIESDRPR"
     gene            27417..28115
                     /locus_tag="PK576_01795"
     CDS             27417..28115
                     /locus_tag="PK576_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60355.1"
                     /translation="MSVVQPLLDLQEIDDRIRKLQQEIHDIPQRKAQEQERLTSVQEV
                     LTRAQAELKAAQVRVSEAELEVKARQDKIQSIKQNQVLLKTNKEFQLYNLEIAKLEGE
                     IEAYEARQLAAMDDVIPVRHRVAEAEAKLKEDQAAVDADLTELDARLAAVRETLAAEE
                     GARVEAAKRVTPQFLLYYERLRTKRWPVVVALDEGGVCKGCNLVQPPSVGQMVRRNQG
                     IVPCQMCGRVLYQD"
     gene            28180..28546
                     /gene="rnpB"
                     /locus_tag="PK576_01800"
     ncRNA           28180..28546
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK576_01800"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(28927..30063)
                     /locus_tag="PK576_01805"
     CDS             complement(28927..30063)
                     /locus_tag="PK576_01805"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE60356.1"
                     /translation="MNLIPPTLLHPNRTLAGPDKILHLTQEAAGFGPRGLLVHGASLQ
                     RSGLLDAILAQPPSGMTVHTWCHAGGEPTVHAVEALRTAIRTERPDWVAAVGGGSVMD
                     LAKAAAGLADAPESVTFYMSHASLIPPAPLPLIAAPTTAGTGSEATGVAVLTDPERTL
                     KQSIRHPSYLPRLVILDPRLLRDCPPDTLAAAGLDAFVQAFESYTSRHATPWTRVLSE
                     LALTRIAQNLLPLYRGDADRAAAMLEASFLAGVALSHARLGVVHGLAHPLGVRFHAAH
                     GLACACCLSACLAFNRPVLSDTLRDLRDRLGLDIENQTATWLETMSLANPFVGKSLPD
                     REAVIRETLASGSTAANPRPVSADDVSDLLDAILGHDALPAAPY"
     gene            30235..31515
                     /locus_tag="PK576_01810"
     CDS             30235..31515
                     /locus_tag="PK576_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE60357.1"
                     /translation="MSRLRFWNERAETLSRDELTAVQLAGLKWTVNHVWTNNAFYRDR
                     LAAHGIEPGDIRSLDDLRSLPFLTKDDFRDQYPLGMLCMPQDTIREMHMSSGSTGTPV
                     VMAYTEADLTQWAECMARCQVMAGLQPGDAIQIMPTFGLFNGGFGFYHGARLAGMFAV
                     PASSGNTARQIMLLRDFKVRAMGSVVSYAARIMEILDAKQIELPHLKIGIFGAESFSD
                     EMRNRVESRLGIRAFDIYGMTETGGVGTTGMDCEARCGIHVWEDHYLTEVVDPQTGAP
                     VPDGAVGELVFTSLTREAVPVIRYRTRDLSRIVSREPCACGRTSLRIDRITGRCDDML
                     IIKGVNFFPKQVEQALMEIPGVLPEYQIILEECHGVTDVRINVEVEEGVTGYQVVKHL
                     KKRLGFSPKGDVFKPGTLPRGEGKAKRVFREVRG"
     gene            31622..32695
                     /locus_tag="PK576_01815"
     CDS             31622..32695
                     /locus_tag="PK576_01815"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOE60358.1"
                     /translation="MRYLTYVGCYTDEKKEGIHIFETDSETGAFCPVGLVGGIENAIH
                     LTLNASRTRLYAGLGMPEYGASAQNGAVAAYEVQGDTLRLLNHCPVGVTPPCYVALDP
                     SEQALAFAEYTNAVAGVFELAEDGRFTDAPAVTVQHEGEGPSKPRQDKAHAHCAEVTP
                     DGRYLCVVDLGLDQVRVYDFPERRGGLRERSTLTIRSVGGAGPRHILFHPNGRFAFVL
                     HELNNTISSFRYTGEEFVLVQTVPMLPPEFKAYSKAAAIKLSADGRQLFGSNRGHDSI
                     AAYDLDPDSGRMSLLAISKLVGEFPRDFEFLPGGKFVLLGHENSNELFSYAYDAKSGV
                     FTPAHGPFSLHRPVLVRFGVRRS"
     gene            complement(32936..33958)
                     /locus_tag="PK576_01820"
     CDS             complement(32936..33958)
                     /locus_tag="PK576_01820"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOE60359.1"
                     /translation="MNPILGMLIFTCGGLAGATFLLPARYVKGWAYESWWFVYVFIGL
                     IICPPVICTFTVPDFWNVVMSAPNAVLLRCAGFGMMWGVGALAWGLMVRYLGIGLGLA
                     IGCGLCAATGTLIPPIATGHAADLVKDAAALTVLGGVFGSLAGIVFVGLAGKLKEDEL
                     PEEEKKKAVMEFDFKKGIITAIIAGIFSAGMNFGLQGAGVIEEAAVKAGANPCWRGMP
                     VIMVVLWGGFIVEAAWCFQQNWKNKTFGDYAKPSLRNILLCAAIGIIWVMQFVCTKAG
                     EPLMGDLKYISFAIMMASTILFSTIIGVMTGEWKGTSAKTKAYLAFGTAVLVVSFCVI
                     SFASKP"
     gene            complement(33955..36132)
                     /locus_tag="PK576_01825"
     CDS             complement(33955..36132)
                     /locus_tag="PK576_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60360.1"
                     /translation="MKKTCVVLAVAAFAAIGAFADVVIENPRFRFVLGDDAKAKSLVL
                     KATGEELLDLNEDIRAFAVIQNRPFNNEVKLTFPNKRTEYRADRVRRAGDFLYIGFEL
                     ISYEAQLKVTEAADYVTFELVDFPLGPRGSNGLKMTYPPVDVMKILDLPVKNRKYFGG
                     WLNVSWDDAAAVAVVAAEPYIWIDNSRRHGFRLMTAEAHRNLKLRGAKTALVVAATGN
                     FMDAMDAFERDLGLPRGVQSRRSQEIKSSIYWSSGVRPSTVDRHLAYAKQGGFRNMLI
                     YYPAICKGAKGDGGYGGIADYELLDEYVNGYDSLKEMLAKIKAAGITPGLHVLQTFIG
                     FKTRYVTPVADPRLNLTTHFTLAKPLGKEDGDVWVHEDPSNCPTNEASRILAFGGELI
                     SYEGFTTERPYKFTGVKRGAKETTIVEHPNGQIGGILDVCEFGGVSCYIDQNTDLQDE
                     VAEKIAKIYNCGFEFMYCDGSEGVNVPQGIHVPNAQYRVWKKFDKKPLFMEGAAKAHF
                     GWHHLSGANAFDVFPPEIFKDMIVRWPLYAAPIMRQDFSRLNFGWWGIYQPSDKSVGT
                     QPDMWEFGTSKGAAWDCPATIQFNLPALEKHPRMADLLEVMRRWEDVRAKKWLTDAQR
                     KALKDSKREFHLYLDDKGAYTLYEIEMLPASAEAKLLRGFVFERDGKRVVAYWHTRGE
                     GEFTLDWAGPKTLKADKMRYLETPMTKAEIAKAFSTAKEGGTK"
     gene            complement(36135..37166)
                     /locus_tag="PK576_01830"
     CDS             complement(36135..37166)
                     /locus_tag="PK576_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOE60361.1"
                     /translation="MKINLRNEFFSEVKRTLAESGEFKAETFRYSTGVDALKVSNSRG
                     SFTILPFMGQMIWRCDFDGRELTMKSMYEEPKDCVKCFNESYGCFMMHCGLTAMGNPT
                     AEDTHVGHAELPIIRYKEAYIEFGSDELGEYVAVGGKAFHDMCFTYNYEFSPRVVLRK
                     GAAKLDIFVTTKNLKDLPLEYYYLCHINYRPVNGSKIVESPLAKKPIINHEVPADYHK
                     PWANATNKWLAVLDKDYKAQSIVGAKGESYKPEIVNCYFHKPDKDGWAFVKQVYPKGG
                     GVFVRYRPEELPYATRWIARTKDEDAMGMCLPATCEHKGRLYCQAHNQQRTLAPGESV
                     TFHVETGIL"
BASE COUNT         7112 a        10788 c        12159 g         7170 t
ORIGIN      
        1 cactccaggc ggagggtgta tcactctggt tatcccacgc gcgcactttc cagtagcagc
       61 gttgcgaggt cgtgagcggc ttgccggcat acaccacgtt cagcgattgg tcagaagcca
      121 cgcggccgct gtcccagaga tcgccctgat tgtcgcgtag ccgctccggc gaggaggcca
      181 ccagaatctg gtaagcggtt tggcgcaggc cgcgcaccga gcggcgtgcg gcggtcaatt
      241 tccaactcag gcgcggggcc gggtggtcga gccccacggg gtcctgcagg tagtcgaccg
      301 tcaacgcctg cggagcggcg agtgcgccgc gctgacccca cggtctctgt gcacaacctg
      361 cgacgagcac caaacccgcc aacatccatg aaatctgttt catcttttct cccctcattt
      421 caacagcagg atagcaccgc cctgcgtggt aagcaagagt tgggtgccgt atgatcaaat
      481 tatgcgcgga ccggcaacac gcaggcggct gcgaaatccg gcagtggaat gacgttgatt
      541 gggcagccgt aggccgtttc taaaagatcg gggcgtagga cttcggcggg agtgccgtcg
      601 gcgagtttga ccccttggtt cagcaggatg atccgcgaga aaaactggct cgccaaggtg
      661 aggtcgtgca ccaccatcag gatggtgatt ttaccgtcgc ggttgaggcg taacaacagc
      721 gccatgaggt ctacgcggtg acgcaggtcc agatgcgagg tgggctcgtc cagcaacagg
      781 atctcgggct gcgccgccaa ggccacggcg atggccagcc gctggcgttc gccgccgctc
      841 atcgctgtca gtgcgcggtg acgcaggtgc caggtgccgg tcacgttcat cgcttcctgc
      901 actgcatcgt gatcggcttt ggaaggggag ccgaagcgcg gcagataggc ggtgcgcccg
      961 agcaacacaa attcgtaggc cgcgaaagaa atatcctgcg gaagctcttg cggaaccacc
     1021 gctacatgcc gggcaaattc acgtgcgttg caacgggcgg gatcgccgcc ggcaattctg
     1081 cgcgaaccct gcgacggaag ttgaccggtg atgacgcgca gcagagatga cttgcccgcg
     1141 ccgttcgggc cgaccagcgc ggcgcactca ccgcgctcaa ggtgcagcga gacatcggtc
     1201 agtaccggct ggccgttgta ggtggcatgg atgtgttcca agtgaatcat ggctcggttt
     1261 ttctatccac agattttcat ggagtttcat gtgtttcatg gttgcgcagc agcagatgga
     1321 ggaaaaaggg accgcctgaa agcgcggtga tcacgccgac cggaatttcg cggggcgcaa
     1381 aaagcaaacg cgcaagcgtg tcgcacacga ccaaaaatgc accgccgcac aacgtcgtgg
     1441 ccggcagcaa tcgacggtgg tttgcgccga ctacgcgacg caacgtatgc ggaacgatta
     1501 aaccaacaaa tccgatgcat cccgcgagcg agacggcggc ggaggtggcc agcgcggcgg
     1561 caccgagtac cagtggcaaa acgatgcgtg tttccacgcc gaggtggcgt gcggtctcgc
     1621 tgccgagcag cagggcattc aacgcagggg tctggctgaa gagcagtgcg gcggagatgg
     1681 caatcagtac ggtcacaatc agcacgagcg tccatgacgt cgcctgcaag ttacccatca
     1741 tccaccaagt aatgctgtgc aaagcgagcg tatcggccaa ggcgatgaca aacatcaaca
     1801 ggcttgagag catcgaactg gcgaccacac cgacaagaat cagggtgttg ggcgagtagt
     1861 gaccggcacg ccctgacatg aggcagacca gcaacagggt caggccgccg accacgaaag
     1921 caccggccgg caaggcgagc ggcgtcaact gtgcgaggcc ggtcacgatc atcaatgccg
     1981 cgccgagcga actgccgctg ctcacgccaa gcacgtaagg ctcggccagt gcgttgcgca
     2041 gaatggcttg cagcgcgcaa cccgagagtg ccagtgccgc accgaccaca aaaccggtca
     2101 cgacgcgcag caaccgcaac tccagcaatg cgcgacctgc aggcgttgag gtgtccggca
     2161 gtccaaatcc cgccgggccg gccagcaggc acaggatgag caccacaggc acggtgagga
     2221 gtagcaggca ggttgttgtg cgggaggggg tcatgggcga tccggggtag gggactgttt
     2281 ggcgaatcgt tcggcgtcct gatccagaac cgctttgatt tgtgcgatcc cgtctagcac
     2341 acgcgggccg ggtcggctta cggtgttcag atcggtaacc gtgtagacgc ggccggaacg
     2401 tacggcgcgc aaagaacgcc agccggtctg tccggcataa atctccagtg gtgggcgtgc
     2461 ggttgcaaag atgcagagta gcagatccgg gtcttgctcg atgatccatt cgagtgagac
     2521 gcgcaggtaa ttggtctcaa gatcatgtcc ggcactgaca ccgcccgcga gttcgagcag
     2581 ggtggtgaca aaggcgttac ggcctgcggt gatggggaca tccggcgcaa agagcagcag
     2641 cacgcgcggg cgggcctggc atgaagaggc cgcttggcgc gcgctctcga tgccgctctg
     2701 gatttcgtcc gccaactggt tggcgcgtgt tgagcggtcg cagagcacac caagctggcg
     2761 aagcgcgtca gggatctggt cgagccgcgt gcagggcaca tgcaccaccg ggatgttcag
     2821 cgccgtgagc cggtcgcgga gcgcgggatc ggaaagcacg gtttccagaa catgcgtggg
     2881 gcggacggca agcaagggtt cgagccacgg gacggcgaag tcgccggtga cgggaatttc
     2941 actcacaacc tccggcgggt ggtcacaggt cgccgtgcgt ccgaccagaa ggtgccccgc
     3001 accgatcgca cacacgcact cggtcaggtg gggggcggtc gagaccagcc gcatgccact
     3061 gtcggcgggc agggggtctt gccggatgca cgccgtcagc agcgacagcg acagcccgca
     3121 cacaatcacg cgccgccatt ggctggtttc tggtttctgg tttctggttt cattgcttgc
     3181 ggagcgccgg gaggggcaag cgtctgcttg cccggcggcg gtcatgccgc catcccgtaa
     3241 aaaacctctg cgtctccgcg tctctgcgtt aaaaactctc aactttcaac tctcaacttt
     3301 caacgctcat tttcctgcga cgggacatcc gcgctcccga acttaggaac aacgcaagga
     3361 cgagcagatg ctcgtccccc cccccgtttt gcggttaaaa agtgaatgtg gcaccgacct
     3421 tccagaaacg gccgcgaccg ggatagtaat agtcgccgct gaagctagac cagccggcgt
     3481 aatcgcaata gtcacggccg aacacgttat cgacgctcag tgttacacgc agaccctcga
     3541 tgaaggaagg ttcgtaccgc aggccgcaat cgaccaacgt gtagcccttg agcggtggcg
     3601 cactgttgtt gaagtcgctg ccgagatact ggcgtgtgac gtagcgcacc ccgcccatga
     3661 gcgcgagact gtgcagcagg tagtagtcac cgttaacgct gagggtgtgc gtcggtacga
     3721 gcggtacgcg gttatcgtca tacgggccgc catcgaactc cgcgcgcacc gcgctgtaag
     3781 cgagcgtagc ggagccgacc cgatcgcgcg accagtgcag tgcggcgtcg aggccgatgc
     3841 ggtccgtgtc gcccggcgag ttgcggttct ggaaggtggc ggggtcgtaa tagatctcgt
     3901 cgtccatgtc catgtgatag agcgagagcg cagccagcca ttcgttggcg atgcgggcct
     3961 ctgcgccgac ctcgaggttg aggccggttt ctggggtgag agcgagattc ggtgtgcccg
     4021 cacctgcgaa gatctcgtcg atgaacggtg catgatagaa gcgccctgcc ttggcgtaaa
     4081 ccttggaggc ttcagtgggt cgatagagca gggcggcttc aacagccgac tcacgcttgc
     4141 tgtatgaggt cgtcgaggcg gcattcttga cgcggttgtc aaaccactcg gttcgtccgc
     4201 caagtgtcag cgagaggtca tcagtgaaga agaactcgtt ctgcgcatag ccggcgatac
     4261 tggcgcggtt gaactcccaa tcaagcaacg aggtgtagaa aggcgaattg aaatccgaat
     4321 agaagtcggt cacgtcatac cgcaggtcgg tgccgagtgt gaggattgag cggtggccag
     4381 cgatacgtaa gtcatgtgtg tactgcggct gcagcgcgta tgaatcgatc tcgctgccgt
     4441 agaaggcctg actggtggca ctgagccagc ggctgtcggt ctcacggcgg ctggccgttg
     4501 cgttcaaatc gaaaatgccg tcggcgccca cggtcgtacg cccgcccaga cggatgccgt
     4561 agctctccag ttcactgcgg tctcgcggtg tggtggaact gcgcggcgcg ttgtgatagc
     4621 gctcgcggga gagcgtgccc ggtaggccgt actccgcgtc gtggtagaag gtggagagcg
     4681 agaggaaacg atcctcgccg aaatctttgc gcagcgcggc attcaactga taggtttcat
     4741 aatcgctgtt gtcgcgatag ccgtcggact tttcccagtc cgcgctggcg cggtaggaaa
     4801 cgccgtcctc gccgaacatg ccggtggtgt tgacgaaagc cgagtaggtg tcataggcac
     4861 cgactgaagc gccgagcgtg gtctgttgtt cggcagtcgc gtcgtccgtg atgatgttga
     4921 tcacaccagc cgaggcgaag tcgccgtgaa gtacggtctg cgatccgcgt ataatctcga
     4981 tgcgcgagat ggatgccatc ggaacgcgca gtaggctcgg cgaagacatg tcgggattgt
     5041 tgaggcgttc gccattgacg agaaccagaa cgcggccgaa ggagctttcg ccgaacccgc
     5101 gcatcgaaag ttgcgtgagc gcgggattgc tgttaagtgt gcgcagggta acgccgccct
     5161 gcttttcgag cacttccgcc gttgacgagc agcccgattc agcgatttcc cgcgctgtga
     5221 tgacctgcac ggaggcgggc atctgcagcg aggttttacc ggttcggctg gcctgcacga
     5281 taatcggcgg gatggcgttg gagacagacg gtgctgtcgg tgaagactcc ggggcctcgg
     5341 gggttgtttg gccgagggtg gtcaggccgg ttgcggccag acacgccgca aatgtggcgg
     5401 cggtaaagcg gtgagacaga gacatgatac gtatcctctt acgcgaaggt tggggcagag
     5461 ccaacgcgta cgaggccgca ggggcgagag cagaagcagt tccggaaacg gccggcgaca
     5521 ggcctcggct cctcggacgc gaaggagtgc cgcagcttgc atccggcccg tcttctggct
     5581 tacggcttga ccttaccccc gccttctctc cctgagggaa atggcatccg ggggcttgta
     5641 gccgattaca gcggcgcgac cgcgcccgat tctcacgggc ttccgatagc cggtgcaaag
     5701 tacgggggga gcatagcgca gcgggcgcgg cctgtcaatg ccgcgccggg cgcggaattg
     5761 acgaggagga gttgaaattt aggatttagg attttaggat ttaggattta ggagttagga
     5821 gttttggggg aaccgccatg acgccaagaa cgccaagttt ttttgggggt tgagcaggcc
     5881 gcgggtcgcg ggtcgcgggt cgcgggtcaa cctctcttgt tgcggccacg cagtggctcg
     5941 caatcgtgag agtaaaagca aggatactgc catcgcattg gttgcagcta aattcaaaag
     6001 gccgctcgtg attccgatgc gcggctgggt tgccggagga tcgcgtcgcg tgtacgtgta
     6061 cggcacccgg gctcgcggaa cgccgggcaa gcgtctgctt gcccggacgg gcagacgctc
     6121 gtcgctgcgg gcgagggtcc cgtgttcccg actttctact cgttcccctg tcttgatttc
     6181 atgaagtttc ttgtgtttca tggtgcgaaa agcttctggt tgttgccaaa aggtgttccg
     6241 atgcgtatac tgttggcggt tttatggata cggacaggaa gtggggtagg gatgagacag
     6301 ggtttgaggg tcgctttttt ttgtctggcg gtcacggtcg gcggagggat ggcagggttc
     6361 ggacaggatt tctcgatgtt gtccgaggcc gaacggaaac aggtgaacgg atggatggcg
     6421 gagcgggccg ccattctggc ggacgcgcac aaaatcgaaa tcgaactcga tcaggcgtgg
     6481 ggcaatccgc agttcacttc gccggaagtg gaggcgcagc gcgagcggta ccgccagttg
     6541 cagcacgaat tgatgatgac ccagttgaca ttgcggaggt tggtcgagaa actccccgca
     6601 gcgcagagtc ggcagtcgga tctggatgcg ctccggaacc gtgcggaggc actttcccgg
     6661 cagatcgagg cgaagggcgg tgttaagcgg taagcctgtc gcggcatgtg ttccgcccga
     6721 ccccgttcag gaggatgtga tgacaagaga aaacaaatcg tcgaccggca aaaaccggag
     6781 accgcggatt ctggtcgtga cgccggagat cacctatctg ccctccggca tgggcaacat
     6841 ggcgtgcaaa ctcaacgcca aggcgggcgg gttggcggat gtgtccgcct cgctggtcgc
     6901 ggcgctgttc gatttgggcg cggatgtgca tgtggcgttg ccgcattacc gtcgcatgtt
     6961 ccacattgat gtcggccagt tgatcagcga cgagctgcgc gtctataaga gccgcatgcc
     7021 ggattctcga atccatctcg cggaggaccg ctgcttttac taccgcgaca cggtctacag
     7081 caacagtcag gacgagaacc ccaagctggc gctggccttc acgcgcgaag tgctcaacaa
     7141 catcatcccc tttgtccagc ctgatctgat ccactgcaac gactggatga ccggtctggt
     7201 tccgggcgcg gcgcggcgca tggggatccc ctgcctcttt acggtgcata atattcacac
     7261 gcaccacctg actctagcac aggtcgagga ttccggaatc gacgccgccg aattctgggc
     7321 caatctcttt tacacgcggg tgccgtacaa ttacgaggaa tcgcgcaaca ccaaccagat
     7381 cgacatgcag gcgagcgggg tctttgcctc gcactacatc aacacggtga gcccgacttt
     7441 tctggaggag atcgtcaacg gttggcatga tttcatccca acggcattgc gggaggagat
     7501 ccgcaacaaa caccgtgccg gttgcgcgat cgggattctg aacgcgccgg atgcgtcgga
     7561 caacccggaa acggatccgc acatcgaggt gtgctatgac gcggcgaccc accgtgaggc
     7621 gaaacgcgcc aacaaagtcg cgttccagca caaggtgggg ctggaggagg atccggatgt
     7681 gcctcttttc ttctggccgt cacggctcga ccccgcccag aaaggtcccc aactgctggc
     7741 ggacatcctc taccgcttca tgtcgcgcca ttggaggcag cgtgtgcaga tcgcgctggt
     7801 ggcgaatggc gtgtttcaga agccgttcca tgacatcttg cactttcacg agatgtacgg
     7861 gcggctggcg gtgtgtgatt tcaatcaggg actttcgcaa ctcgggtacg ccgcgtccga
     7921 ctttacgctg gtgccgtcgc tcttcgagcc ctgcggcctg ccgcagatgg tggggcagct
     7981 ttacggatcg ctgccggtgg tccatgacac gggcggattg catgacacgg ttgagcacct
     8041 ggatcccgcg cagaatcagg ggaacggttt cgtgttccgg atttatgaca gcagggggct
     8101 cgattgggcc atggaaaagg cgatcgagtt ttacaagtgc gcgccggagg tgcgcgagcg
     8161 gaacatcgcg cgggtgatgc gcgagggcaa ggcgcggttc aatcacgaaa cctgcgcccg
     8221 ggcctatatc gagatctatg agaagatgct gaaccgtccg ctggtgcgtt cgttcgaatg
     8281 atgcttccgt ctatctatct gcaaggagat tcgaacatgc cagccacatt acgccgcccc
     8341 cgcctgctgg atgatccgta tctcctgccg tatgcggaga tcatccgtcg gcgtgccgaa
     8401 ctggccgacc ggcgcgaggc ggatctgtcc caaggtccgg gcaagctggc ggaattcgcc
     8461 tgcgcgcacg agtattacgg gcttcatcga actgattcgg gatgggtctt ccgggagtgg
     8521 gcgccgaacg ccacggcgtt gtggctggtg ggggatttct cgtcgtggca gtgtcaggag
     8581 cctttccggt tgcaacggat cccggggcgg ggggtctggg aggcgcatct ggcgccggat
     8641 gcggtgcgcc acgggcagta ctaccggctg gagatggtgt gggacggcgg gcgcggcgaa
     8701 cgtattccgg cttacgcgcg ccgggtggtg cagaatcccg agaccttggt gttcgcggcc
     8761 caggtgtggg atccggagcc gtacgtctgg cgcaatcccg acttccgggt accggaccgg
     8821 gtgccgctga tctacgaggc gcatgtcgga atggcgcaag agcgggaggg ggtcggcggc
     8881 tacacggagt tccgggagcg gattctgccg cggatcaagc gggccggata caacacggtc
     8941 caactcatgg cggtgatgga gcatccgtat tacggctcgt tcgggtatca tgtgtcgagc
     9001 tacttcgcct gttcgtcccg tttcggcacc cccgaggaat tgaaagcgct ggtggatgcg
     9061 gcgcacgggg aagggcttgc cgtgatcatg gatatcgtgc attcccacgc ggtcaaaaac
     9121 gagcgcgagg ggctgtcgcg tttcgacggc accctctacc agtatttcca tgacggcttg
     9181 cgcggttggc atgaggcgtg ggattcgcgc tgtttcgatt acggtaaaac cgatgtgctg
     9241 cactttctgc tctccaactg ccggtactgg ctggatgagt tccgcttcga cggattccgt
     9301 tttgacgggg tcaccagcat gttgtatctg caccacgggc tcggagtgga ttttatggat
     9361 tacagccagt atttcgatgc cacggtggac gaggacgcgt ggatctactg tcggctcgcg
     9421 aaccgcgtga tccatcaggt gcggccggat gcgatgaccg tggccgaaga tgtgagcggc
     9481 atgccgggcc ttgccgcacc gctggaggat gacggcgcgg gattcgatta ccggatggcc
     9541 atgggggttc ccgagtgctg gttccgtttg gtgcgcgatg tgcgggatga ggattggagc
     9601 attggatacc tgtggcacga attgaccaac cggcgcgcgg atgagcggac ggttaactac
     9661 gtggaaagcc acgatcaggc gctggtgggc gggaaaaccc ttttcttcca attggttgac
     9721 gcggcaatct atgatgccat gctgcgttcg atgcggcatc tcgaggtgga acgcggtgtg
     9781 gccctgcaca aattggcgcg gttggccacg ctggcgaccg ctggccacgg gtatctcaat
     9841 ttcatgggta atgagttcgg ccatcccgag tggatcgatt tcccgcgcga aggtaacgga
     9901 ttctcctacc ggtatgcgcg tcggcagtgg acgttgcgcg acaacccgga gctcctctac
     9961 tggtgtctgg gcgagtttga tgcggcgatg ctggagatgg tcacgcggta ccgggcgctc
    10021 gaagggaccc tgccgcgtcg gttgttcgtt tccgaccagg agaagatttt ggtgttcgaa
    10081 cgagggccgc tggtcttcct cttcaacttc cacacccaca cttcggttgc cgatttcaag
    10141 gtgttggtgc cgcccggttc ctatcactgg gtgatggaca gcgatgaaga gcgtttcgga
    10201 ggccagggcc ggatcgcggc agggcagtcc tttgcgctga tcgccgaaca acgtgaccgg
    10261 gagttatgcc agaccctccg cgtctacctg ccctgccgga ccgcgatggt gctggagcgc
    10321 aggatctgaa agatccgaac cgcggtcacg tcttgtgcac atcgacgcgg gttgcgccgc
    10381 gccgctcata tggcggggga ctcaccactg ccttgccgtc atccggcggc ggcatggcgg
    10441 aggatcggcc tcccatgaag gggaagccgt caaacgggga ggtcgcgcgt cccgcgcgct
    10501 cttccgccag aaccatgcgg tattcgcctt ctgcggcctg atagatgaaa taagcgatca
    10561 gcaggcggat cagcttgaat ccgtcgaagc ggccggtgaa cagtgagtac agcaccgaga
    10621 ggcccagcaa cagggcgagg aaacgtccga tgcgcgaggc gacccaagtg gcgcgaaggc
    10681 gcgtcatgaa aaattgctgc aggatcgcgc gtagcatgcg tccgccatcc atcgggaaag
    10741 caggcaacag attgaagacg aagagcatcg cattcaacgc gccgatttgc aggaagagga
    10801 accggtccca accgctcgca aacagcacgt ggccgtcacg gtaggtcacg tccggcagca
    10861 acaggcccgc cagggccagg cccagactga ccgcaggacc ggccgccgcc atgagcaact
    10921 cttgccacgc tttgcgcggc atcgagagta acgtggcaca cccgcccatc atcatcagcg
    10981 tgatgtcccg gacacggcac ccgaaggcca tggcagtcag gctgtgtccc agttcgtgca
    11041 acgtgataga gacgagcagg gcgatgccgg cggcgatgcc gtaaagcggg ttgccgtagt
    11101 tcccgatcaa caggaacatc agtatgatca gggatgcgtc cagacgaatc gggataccga
    11161 aaacagtgca gaggtggtat gaggatcgga gcataagagg gtcagcggtt agagggtggt
    11221 gacgtatagg gtggattcag ggtgaggagt cagaatgtgt gcgtcggctg tggctggaag
    11281 gagaagactg gagcccggag ggagcgtggc atgttggcga ttcccggcgt ctgccaccgt
    11341 cacgcgaccc gctgccgcga agagcgcgag gaaggtggtg ccgtccggcg ccacacgcaa
    11401 cggctcgcgc agatccagtc ggcgcacggt gaatacgggg gaggtgacga tctcggacca
    11461 gaccggcgcg gtgcccgtcg gacaaggcaa ctgcggcgtg aggagttgcg gcgggggaag
    11521 ggaccagtcg atcgacgcca accccgggcc gatatggagc ggccgcgccc ggccgtcagg
    11581 gccagtacgg ttccagtcga acaaccggta agttgtgttg gcgctctgct gcacttcata
    11641 aatcagacac cccgcaccca tggcgtgcgc cattcctccc ggaagaaaga gcgcttgtcc
    11701 gggttgcggc tcgaatcgga gaagctgttc cgccaccgtg ccatccgaca gggcggcgcg
    11761 caaaacttcc ggggttacct gcggctcgaa cccggcgtag agtgacgcgc cgggcgtgca
    11821 gtcgaggacg acccacgctt ctgttttagg ttctcctccc gtacgtgcgg cgcacgccgc
    11881 attggggtgc acttgcacgc ttaggcggtc cgacgcatcg agcagtttga acagcagcgg
    11941 gaaacgcgcg ggatccggtg cgcgcgtacc ggtgagcgcc gcgccgtatc gggcggtcag
    12001 ttccgccagc gtttgtcccg cgaaaggacc gcatgaaaca acgctcatgc cgtccggatg
    12061 agccgagatc tcccaggact ccgcgcacac cgcaggcaca ccggtacggt ggaaaaacgt
    12121 ccggaaacgg tcgccgcccc acacgtaccg tttatagacc ggttgaaaaa ccaagtgtga
    12181 gagagaggga ttcattccgt ttgccagtgt agcgggggag ggtcgcgaat gcaagagagg
    12241 agccagcaga acgcgtcggc ttacgatccg cgacattcga cctgcggaaa ccaaagagcg
    12301 ctgaaaggtc acctgttctt cgttgtcgcg cacgataacg ggaaatcgtt agtctgccgc
    12361 atgggaaatg gggtatactg tgtggtatcc gggatcgaga tcggaagcgt gtctggtcag
    12421 cggttcggcg ggaggggttc tgggcatgag acatttaccc gcgttattgg ctgttttttg
    12481 ccttgccgtt ctgtgtcttc gagccgggga gggggagtgg caacggggct ttctggggaa
    12541 ggcggagttc aacggcacgc tgaagggatc atcggccgaa acggaatggg aactggtttt
    12601 cacgcgcaag ggccgggcgc tcgatgcgac cgggggggtg catgaggtca atcagccgat
    12661 attcaatcgt gaagggctga ggatggccgg ggggcgcgaa accgagtaca gcgactcgtc
    12721 cggggcgatc acgaatcacg ccgggatctt tcagacacgc gagatcttgt cgttgccccc
    12781 ggtcttgacg cagcggatgc cggaggatga tttcactgtg ctggttacct tttcggtgtt
    12841 taaaacagga gggctctacc agacggtgtt cgagcaaacg cggcgtcaac gctggtggcc
    12901 gacgctgcac ctcgggttcc gttcgcagca ccgcaaccag cttacgctgc atcatgccga
    12961 ccgtacggat tcgagcgcga agcgttttgc cgagaccggt gttttgccgg agaccggacg
    13021 cgtctacacg gttgtgctga cccgtcagcg cggtacggcc aaagtttggg tcgacggggt
    13081 caagcgggct gaactggccg ggattccggt cgaggatgtg agcgactgga atctcgggtg
    13141 cggcgggccc gaacgtgcgc tggacggcca tctccgcact tttgcggctt gggatcgtgc
    13201 gttggacgag gaggaggtcg cagccatcga tctgaacgac ttggccacgg ccggtttggt
    13261 ccgtgtcaca ccgttgcgct atatccgcca tttggcgcgc ggcttcgaac cccggtccta
    13321 ctctttccag gtgcggaatc tttccgacac ggcgctggag ggcgtggcgt tcgaatggga
    13381 gatcggagac cagcggttca cgcagccagt cgggcggttg gaggctcagg gcgagcgggt
    13441 gatcgacgtg ccggtcctga tcacgctgaa acccggggat acggttcaga ccttttccgt
    13501 cactgcggga ggcaaagcgc tcaaccgcca gtcactgcct gccaccgtga cgcctgcgcc
    13561 ggttccggtc aatcagctcc agacctcctc ctggtgtcag ttcttgccgg gcgagccggg
    13621 ttacaccatg gcgacggtcc ggcattgcgc cgacgcgatg ctggcgggac tccactgggg
    13681 gccggttcaa ttgcggtgga ccggttgggc gcgcgccgac catccggagg accgggcctt
    13741 cacgcctcat cagaagcgct cctacaatga tttttatttc agcgaacgga tgcgggagca
    13801 gatgcgtgtc tgcacggacg ggatcgtccg gcggctcgcc ttctatcccg cgctcacgca
    13861 catcgacatc aacaatgaat acaatccgaa ctttcaggtc aacttcagcg agccggcccg
    13921 acgttacgct gcagagcatt tcggcctcga ccttacgcct tggatgggag acggcaaacg
    13981 cgagctggtg cgtcccatcg ggcggctctc gccggtgttg gcgaactatc cgattcccgc
    14041 cagccggatc attccggcgt ccgatccgct ctaccgctgg catctcgacc gcaaggggcc
    14101 gcacggtgat tcggaaagtc tgctcaacca gcatatcgct acggccatga ggcgcgagcg
    14161 gcccgatgtg ctctctgtgg tggccccgct tacgcgtcgt ctctccctgc gcggatatga
    14221 cgaccgtctc gacatcgcgc agacctggtg ctacacgccg gggttgcgcg tagtctggct
    14281 gcaggaggcg ctcaccgcgc atatccggca tcagccgcgc atgcgatcct cctcctttcc
    14341 cgcctttctg catatgaacg cggggcccta tccggccatg ccgtccggcg acattttcgc
    14401 cgcgtcctgc tgggcttcgc tgatccggcc cacctccatg ctggttctgt ttcaggcgct
    14461 tgagatgatg cgggggcacg ggctgcccaa agcggagatc gatgcgctgc ttggttcgac
    14521 cgcacccaat gcggaagaag cggcgcgtgt catccgggcc aagcgtctct cctttaaggg
    14581 cgatcatccc acgcttgcgc cgcgcgtgcg gcgtctgctt gcggaggagc ttccgccctt
    14641 ccgggctctg cttcccgcgt ggagcaacgc tccgcgtcag atcgcggtct attcgtgttt
    14701 cgcggacagc ctgtttgcgg accagcgctg gccgaccgat tacacgccgt tgctggcgga
    14761 actcacccgt tccggggtcg cctatgatct gcttttcgac gaggacttca cgcgtgatcc
    14821 gaagctgctc gatgggtacg acatggtgtt tatcccgaac ctcacgtatc tgttcgaacc
    14881 ggcgtggcag gcgctccgga cctttgtgga gacgcccggc cgtgtgctgg cagtgccgga
    14941 tacggcgcac gtttttttcc cgaatgccgt gaccgtgcgc gacaccggcg gggcgctgtc
    15001 cgcagaggag cacgcgcgca tcgtcgccga ggtcgaatcc cgatacggag cggacagcgg
    15061 acgtccggcg gtgattgagg cgttggacgc gcggttggct gaggccgccg cgcaaggggc
    15121 ggacgcgggg ccctttgccc cgttactgga ccggctcggg cggttgaacg cccgttgcct
    15181 gatgccgcac acggcactga acgtgactag actggacgac acgcacttcc tttcggtggt
    15241 caacgacttg cgtacatggg ggccgatata cggtcatttc cgtgtgttgc cggaacgggg
    15301 agtgcggcag accggcgacg tgctgttccg cagcgcgctc ggccggttcg cttacaacct
    15361 ccgcaccgcg cggcgtcttg cggtcacgac ggggggtaac ggacagtcgc ggctatcgct
    15421 cacgctcgat ggcggtgacg ccgccatcct gctgctgagc gatcacgagt tggggaggtt
    15481 ccgtgccggg atcgtgcgcg aggggacata catgcgcgtc accgcgcagg cctgcgacac
    15541 tacgggccgc gagatcgatg tctccatgcc gtgccgggtc gcggtcgagg gcattccgga
    15601 ctactccggc gtcgcgcttt tccgcaaggg gaaattggac taccgttttc ccttcgacgg
    15661 tacgttcccg acggtcaccc tccgtgagga ggccactggc gcgaccgtca ccctgcggac
    15721 cgcgacactg cgcgattaag gcgctttcga agagaacgct ggggtttcag gattgctttt
    15781 acgatcagaa aggattttaa tactcgccgt acaagtgcgc ggatggtttc tgacgcaccg
    15841 gatacgtatg agggaaaggg agaatatgaa acggatgttg atcggcaccg cattcgcgtg
    15901 tgcgctggcg gggatgggta tggcggccgg agttctggaa gagggcttcg tctcgctctt
    15961 caacgggaaa gacttgtccg ggtggactgg tgcgacaggc gggtacgcgg tcgagaacgg
    16021 ggttctgttc tgcaaagaaa agagcggggg taagctgctg accgagaagc agtacgacaa
    16081 cttcatcctg cgttttgaat ttcttgtcca aaccaactcc aacaacggac tcgggatccg
    16141 ctgcccggca gagggagacg ccgcatacaa cggcatggaa ctgcaaattc tggatgattc
    16201 gggccccaag tacacggcgc tcaagccgta tcagtatcac ggctcgatct atggggtggt
    16261 gccggccaag cggggacacc agaagaaggt gggcatgtgg aacttccaag aggtgcgcgc
    16321 catcggttcg cggatcacgg tgatcctgaa tggggaagtg attgtcgatg cggacctctc
    16381 aaccatcaac gagacgatgg acggcaagcc ccatcccggc ctgcgtaata ccaaagggca
    16441 tatcgcctgg ctcgggcacg gctcgcgtgt ggattggcgc catatccgca tcaaggaggt
    16501 cccggccgat tatacggtgg cccggccgac cgataaccgc gctccggaag ggttcaccct
    16561 gttgttcaac gggaaagaca tgaccaactg gaagggggtc accagagaag agagattcga
    16621 caatcctctg gtccggcaac aggccacacc ggagaaacgc gcggccatgc agaaaaaggc
    16681 ggacgagctg gcacggaagc attggcatat ccgggatcag gccctgtatt tcgacggcaa
    16741 gggatacagc ctcgcgaccg ccaaggatta cggcgatttc gaaatgtggg tcgactggcg
    16801 tctgctgtcg gtccgcgggg atagcggact ctatctgcgc ggctcgccgc aagtgcagat
    16861 ttgggacgcc cacaatcagt ggaatatcgg ttccgggggg ttgtataaca acaaaaagaa
    16921 ccccagcaag gcgttgaaga tcgcggaccg gctgcccggc gaatggaaca ccttttacat
    16981 caaaatggtt ggcgagcgcg tgacggtcaa actgaacggc gagttggtgg tcgacaacac
    17041 ggtgctggag aacttctggg accgtagcaa accggtcttc cccaaagagc agatcgagtt
    17101 gcagtgccac ggcgatccgc tggagttcaa gaacatctat atccgcgaac tgtaaccggt
    17161 tccgcttgga tagcgcattg gggacgtgtc cggaaacgga cacgtccttc ttggtgtggg
    17221 ccagcgtgaa aatcaggaga gccgggattt gaaatcctcg tacccaaaca cgcgtgcgat
    17281 ccggatcgcg ccatccggtt cgcgcacggc aagcgtcggg agccggatgc cgttgaaggt
    17341 gttggttttc accatggtgt aatgggccat gtcttcgaag aggatgcggt cgccgggtct
    17401 gaccggcgaa tcgaaggacc aatccccgat gatgtcgccc gcaaggcagg tggggcccgc
    17461 cagccgtacg gtgtgtgctt tgactccggg gtccgcgccg cagtcggcct gtggccccgg
    17521 cacggcttcg ccgtgccagc ccgcatcgcg gaacacgcgc ggacggtagg gcatttccag
    17581 cacgtccggc atgtggcagg cgcacgaggc gtcgagaatc gcgatcggca tgtcgttctc
    17641 caccacgtcc agcacggttg cggccagggt tccggcgttc agcacgcacg ccgcgcccgg
    17701 ttccagatag agggtttcga tctgggggtg gcgctcgcgg aaggcattga gcgtgttgca
    17761 gagcaggtcg agatcatacc ccggctgggt aatcaaatga ccaccgccga agttcatcca
    17821 gcgcatctgg ggcagcagat cgccgaagcg ggcctcgatc acctcaaggg tacgctggaa
    17881 gggggcggcg tcctgttcac aaagcgtatg ggagtggagt cctgtgatgc cgtcgagccg
    17941 ttcgttattc agggtttcgc gtcggatgcc cagccgggaa ccgggtgcac acggatcgta
    18001 caggggcgtg gtgccctccg agtgttccgg attgatgcgc agtccgcagg ggatatccct
    18061 cccgactgag accagtatgt gacggaagtg ccgccactgt gacagggagt tgagcgtcac
    18121 acaatccgca agcgtcgcga tttcagacat ctccccttcg gaaaacgcgg cggcaaaagc
    18181 gtggacctcg ccgccgaact gttcacgtcc cagtcgcgtc tcgtgcacgg agctggcgca
    18241 acagccgtcc agcgtgcgcc gcaacagcgg aaacacgctg aacatggaaa aggctttcag
    18301 cgcgagcagg atgcggcagt ccgtgcggcg cttgacttcg cgcagcaccg cgagattccg
    18361 ctccagtgcg gcggtatcga tcacaaatgc gggtgtggga agaggcattc aggtctccag
    18421 aaaggtgttg ggttgggtga ctggcgaatc gaaatcagca atacgaaacg gattgtttcc
    18481 ttaggcagac agacggccga tgcgctcatt cacggggcac gcgccggatt tggtgcgcga
    18541 gccgcgctag aaacgcgtcg agcagtgacg acatcagcgg tgccgttaca cgggtctgtt
    18601 cgagcacctc ctcatgcgat aggtgcggcc cgctgatccc cgccgccatg ttggtgatgc
    18661 agctcagcgc ggcaattcgc attccggccg cattggcgac aatcgcctct ggaaccgtgg
    18721 acatccctac ggcgtccccg cccatgccgg aatacgcacg gatctcggcc ggggtttcgt
    18781 atcccggtcc ggacgtgaac acatacaccc cgtccgaaag ggacgtgccg ctatcgtgcg
    18841 ccgcttcacg cagcaggttc gtcaactccg gatcgtacac gcgcgactgg tcggggaacc
    18901 gcggccccca gccgggaagg accgggccga tcagcgggtt cagtcccacg ctgttgatgt
    18961 ggtcacggat cagcatgagg tcgcccggtt tgaacgtggg gcggatcccg ccggccgcat
    19021 tggtgatcag caggtttcgc gtgccgagtc gacgcaacag ttcgaccggc agcacaaccg
    19081 gttcccatcc cgccccctca taccagtggc ggcggcccag aaaagcggcc acgcggatcc
    19141 cgtgacgctc gaacaacacg aatcggcctg cgtgaccgac gaccgtactg gcaccgagcc
    19201 ccgggatctg gtcgtaggcg atctccgcga tcggcgggtc tacggacaaa gtctggctcc
    19261 aaccggaacc aaggatcatt ccgcaatcgg gttccccgtc cgtgcagggg ggcggcaggg
    19321 cgcggaaggc gtcttcaaac agtggggtgt tcatggcaga tccgaatagg ttgcgcgtcc
    19381 gccgcaaact gtgaggcggg cgcgggcgtg gaggttccaa cccgcgaacg gtgtgttgcg
    19441 ggaacgagaa cggaaggttt cgggattcac aatccagggc gtggccggat cgatcagcac
    19501 cagatcaacg ggcaggcctg gccgcaatgc gggcacgggt tgccggagta acgccgcagg
    19561 accgcaactc cagcgcgcga cccagtccaa gggcgtcatc ccctcttcct caaccagaat
    19621 tcgccaggtg acgccgatcg cagtctccag tccaatgact ccgaaagcgg cgcggagaaa
    19681 cccgtgtgtt ttcgtttcgg gtgtatgcgg ggcatggtcg gtcgcgaaaa ggccgagcgt
    19741 gccgtccagc accgcctcac gcagcgcgcg cgcatccttg cgggttccga gaggggggtt
    19801 cattcggaag tggccgtcat cagacgggat atcctcggcc gccagcgcga gatgatgcgg
    19861 cgtgacttca gcggtcacgg gcaggccctc tgcgcgcgcc acccgaatgg cggccaccga
    19921 ttcagcgcag gagacgtgct ggatgtgcgt ggcgcagccg gtttcccggc agagacggat
    19981 atcgcgccgc accgcctcca cttccgcttc ggccggaaag atcggccaac cgtttgcgcg
    20041 tgcgagggga cagtcgcgca caaccccctt cccggcgatc gacggaacga cagcgtgatc
    20101 catcacaggc ctattcaacg cgcgggcgcg tgtcatggcg cacctcatca cgtcctcgtc
    20161 cgccaccatg gcgccgtcat cggtgaatgc gaccgctcct gcctgcgcga gcgcctccag
    20221 ttccgcaaga tcgcggccct gacggcctcg tgtcacgcag gccgcaggga ggatccgcac
    20281 cggcacatca tcagcggctt gctcctgcac ccaaaccgcg gaatcgcctg cgggcgaagt
    20341 gttgggcatg gtgacgacat gcgtgaaccc acccgcggcc gctgctccgg caccggaacg
    20401 ccgggtttca gcctgcgtgt taccgggctc gcggaagtgc acatggacat cccaaaaccc
    20461 gggtgccgcg atccagcctt cggcccggat gcgccgcaac ccctccgttg tcgcggcacc
    20521 gccctgtaga ataccgtcgc gaagaacgag atccgcggtc tcatcccggc cttctgccgg
    20581 atcgatcacc cgcacacgct ctaaaatcag gttcaccatg ccgcttaagt ataccggggc
    20641 acaccgcacg gtcaagcgtg cttccagcaa ttttgaacgc acttccgtat tcgtgccatg
    20701 aaacacagga aacgtcatga aatcaagcta gggatatgct cgcgtctgcg gcggcagccg
    20761 ctttcgactc gcatgcggtc agggaaacaa gtaagctatg cctccattcc ggatcagtgt
    20821 gccggaacgc aagcatgcga ggagatggat gatgtcgaag gattctgtga cacgcgagac
    20881 ggtgttggca tttcatatcg gcgggaagat cggaatgcgg atgtccaagc cgcttcaaac
    20941 ccaaaccgat ttgtgtctgg cctacacgcc gggtgtggcg cacgcggtgc gcgagatcgc
    21001 cacccattcc gcaaaagtgt tcgactacac ggcgaagcgc agtctggtgg ccgtcgtgtc
    21061 ggatggcacg gcgatcctcg gtctcggtga tttgggtgcg gaggccagta tcccggtgat
    21121 ggagggcaag gccgtgctct tcaaaagttt cggcgatgtg gatgcctggc cggttcctct
    21181 gaaccattgc cgacagggtg gcgcgaacac cggcaagacc gatccgcagc gcgtgattga
    21241 cgcggtgctg gcgatcgcgc cgatgtacgg cgggatcaat ctggaggata tcgcggcgcc
    21301 ggcctgcttt gagatcgagg atcggctgga cgagttgctg gatatccccg tgtttcatga
    21361 tgatcaatgg gggacagcgg tcatcacgct ttccgggttg atgaactatg ccctgctttc
    21421 agatcgctca atggaatcgt tgcgcgtggt gatcaacggc gcgggggccg cagggatccg
    21481 catcgccgac atgtgtaagg cggccggggt acaggatctt gtcatgtgcg acacccgggg
    21541 cgtgatccac acgggccgtg ccgacctgaa cgcgcacaag cgtcggcatg cgtgcgacag
    21601 tgcggcgcgt acgctgcaag aggttctgcg cggcgcgcat gtgtttatcg gcgtttcggc
    21661 ggcggacgcg gtgacggcgg aggatgtgcg cggcatggcg gagtacccgg cgatctttgc
    21721 catggccaac ccggaccccg agatccggcc tgaaggggtg gccgaggtgc tgggcgacaa
    21781 gccgtatgtg atggcgaccg gacgatccga ctatcccaat caggtcaaca atgtgttggg
    21841 ttttccgttc ctgttccgcg gagcgctcga tgtgaaggcg cgtacgatca acaccgcgat
    21901 gaaggtggcc gcctccgagg cgcttgccgc cttggcacgg cagccgacgc cgtcggaggt
    21961 gcaggcgttg tatccgcgcg aaaatctggt gttcggcaac ggatacatca ttcccaagcc
    22021 gttcgaccgc cggttgttca ccggggtgcc tttggcagtg gctcgtgccg cggtggcctc
    22081 cggggctgcg cctgccgata ctgatttgga ggccctgcag gtcgaactcg ctgcgcgcga
    22141 tgcggcccgc gggtgatcgg tgaacgtgag gtgcggcacg gtacgccatg ccgtttcatt
    22201 tttccctttg atccgagcgc gttagcggac gttgtctggc gttgcctcgg gctcttgcca
    22261 caggaagtcc gtgagcggcg acgaggcttc ggcatcgagt cgcgcggcac gcggtccggc
    22321 gtcgagtccc gcttcagcgg cgcgcacggc gagcgcaaag gcttgcgcca tggccaccgg
    22381 gtcggcggcc gtggcgatcg ccgtgttgac gagcacgcaa tccgcgccga gttcgatcgc
    22441 ggccgcagcg tgcgagggca gcccgagtcc ggcgtccacc acaaccggca cctgcgcgtg
    22501 ggacacgatg atctcgatca tgtcgcgggt acgcagtccc agattggtgc cgatcggtgc
    22561 ggccaacggc atcacggcca ccgtgccaac ctcttcgagc cgtttggcga ggaccgggtc
    22621 ggcattgatg tacggcagaa cccggaaacc ctccctcacg agaatttcgg cggctttcag
    22681 cgtttcgatg gggtcgggaa gcaagtggtg cagattgggt gtcagctcca gcttgatcca
    22741 gtcgtacccg cctgcggcac gcgccagccg ggcgatgcgc accgcctctt ccgcagtgcg
    22801 ggcgccgctg gtattgggca cgatcaggac tttctccggg tcgagttgtg agagaagcgg
    22861 atcccccgca ggatttttat cgaggtccac acgtcgcagc gccacggtta ccagttccgc
    22921 gcccgaggcc tcgatggcct gccgcatcac gtcgtgcgag ccgaacttgc cggttccgat
    22981 gaagagacgc gaacggaaaa ggcgtccgcc gatctggaga ggtttcatag gcttgtcgtt
    23041 cccgaggggt tatgaacagg ggaggggtgc gaaacgtcgc cgcgcgcaac ggcgcggcag
    23101 cgtgttttcc gggtatcagc ctccgcccac gaactggatg atctcgatcc ggtcgcccgg
    23161 cgcgaccggt gtggtttcga aagcgtgtgc ggggatgatg acatcgttgc gttccactgc
    23221 catgtggatt gcgttgaccc ccagagcctt cagcaggtcg gccaaggaat gggccttgcc
    23281 gagcggacgc ggtttgccgt tgacggttac ggtcatgtgc actccttccg atcggcggac
    23341 acggtgagtc ttcgtgcatg tccggggccg gagtccacct cgcggccgat cgccgcgata
    23401 cgctcccgtg cgtacatcgc gcatgtggca ccctcttcgt cggcataagg tttacccggg
    23461 tatagttgat acatccgacg gtacttatcg ggggtcgcgt tcggcatgaa cacattcgcg
    23521 ccgcgcatca ggacaccgtt gcggccttcg ggttcgagcg cgtcgaacgc ggtggtggcc
    23581 gggatatgcg cctgcgggtt gagcagacgc agcagcgcga ctgttttgta atagacgctg
    23641 cggtcttcca gcaacggttt ttccgcaagc ggggtgccgg ggtgtgagag gaagggaccg
    23701 cacccgatca tgtccagctt gagctgcgtg gcgaacagga tgtcctgcgc gatcatctcc
    23761 ggggtagagc cgggcaggcc gatcatgaat ccgctgccga cctggtaacc gaggtctcgc
    23821 aggtcagtca ggcactgcac gcggtgttcg aacgactcat ccgggtggat cgcggcgaac
    23881 agtgcggggg aggtggtttc gaaacgcagc agaaagcggt cacacccggc ggttcggagt
    23941 cgggccaatt ctgcgcgggg gcgcacaccg atggacaggg tgatcgccac cccggtctcc
    24001 tccttgatgc gttggatcat gccgcagaga atctcggtcg tataaaaggg gtcctcgccg
    24061 gattggagca cgaccgtacc gcatccccag tgtttggctt ggcgcgcggt attcacaatc
    24121 tcgtcggcgg acatgcggta gcgcccgaca tgcgggttgc cgcaccggat gccgcagtag
    24181 agacagttgt ttttgcagac gttggaaaac tcgatgatgc cgcgcaagaa aaccgcgtcc
    24241 cccatgtttt cccgtctcag cgtgtcggcg gcccggtaaa tccgttcacg gacccggccc
    24301 tgggccttca gcagagatgc aagttccgcg acggatggca gggcaccctg atgcattttc
    24361 ttgagatgac ggagagaaac catgacatag aagtataccg caaaagggga acgacgggaa
    24421 gcacgtcctt tcgtggacgg gcgcgtccgc ggttatcgca ttcttttgtt tctggtttcg
    24481 gctgtttcgc ttttcgcagt gacgcttgag cgggaccttt catcgggtta tcggggacgg
    24541 tcgctttcga tatcgagatt gtcgaggcac accgctgtct tgtcttgacc gccgacggcg
    24601 aaccggaacc cgtcgatttg ccggagcgtg cggggcaggg gaatggccgt aacgaggcgg
    24661 ctgttcccgt gcgggaggca gactcggaga tcatacgcgc cgcacgcggc gagaccgagg
    24721 ctgatgcgca tttcgacatg aacccaggtt ccggaaggaa gccgcattac ggttttcccg
    24781 tccgcacccg caaggtcgcc ctgtccgttc acccggaaaa ccgccgtttc caccgcatat
    24841 ctccggcgcg gcacattcgc gtccaaaatg gaatactcga acgtggcacc cggttccaga
    24901 tacatatcga actgatgggt ctgccgtccc gagagccttc ttccccacga acgcagggcc
    24961 ggtgtggggc cgatcgactt gtcgttgagc atcttgagcg cgtggcggcc tttcgcagcg
    25021 ttcttttccg taacgaacac aaggagattg ctcggattcc ggccttccgt gtgccagccc
    25081 gccagaccga actttgtggt gcccgcgcaa cactcctcaa agccggttga gagtgttttg
    25141 tgctgatcca acggggcaac cgggaacgtt tcacccggaa ggggttggtc gcccgcgaaa
    25201 cagagcggca gggtccgccc cgcatcggtg aggtcaaagg gaacgaatcc gagcgcatgt
    25261 gccggggagt cgggggaaag cgtgaagtcc cccatgtccg catttcggaa ctgcgggtcg
    25321 gcaaagaccg cgcccgtgtc ggttttcgcc cactcggcga acgttttccc gttgaaggcg
    25381 gtttggatcc tctcgggaga atcgacctgc caccacaaat tggaggcgta ccggttcggt
    25441 gcacccgtgt tgccgccccg gttcgccccg cgccagacga aaaggttgcg ctcgatgcgg
    25501 ttcgtgccga acttgcaatc cgctgtccgg tcgttcgcgt agaactgctg gtcgcgcccg
    25561 tccgcgaaaa tgttgttcac gatcaggttc cccgacttgt cctcactcgg aatcacgact
    25621 ccgctgtcgg tgtcatacgc gaggttgttc gagagggtga ggcaggaaga accttggtca
    25681 aagtaaaagc tgtgaaaccc gtaccgtgcg gcaaccgcgc cgtggacgcg gttgtggcgg
    25741 atcaccgtgc ccggctggag cccgagggtg tagacgccac ccccgtcgga gagcacgccg
    25801 ggcgcataac cgtagatgtg gttccactcg accgtgtttt cgcgcgtgaa actcgggtac
    25861 ggcgcccatg tccagccgac ggagatcccg ctgtaatagc cgtcatgaac cgtgttgtgc
    25921 gcgatgagac accggtttgc gcccccgatc agaatcccga ccgcgccggg ctggtcccgt
    25981 ccataccatc cgatcaggca ctggcggacg actgttccgg aggtctcgtt gtccttgtcg
    26041 cccgaacctt tccagaaggc acccgatgcc acgccgccac cgccgatgtc cgtcatctcg
    26101 cagccatcgg ccgcgcaatc gcggcacccc aatccgaacc gcactgccgt gccccccgtg
    26161 ttccgcatgg cgcagttttc gaaccggatg cggtctgcgt agtccacgtg aatcgcggca
    26221 gggagttcgg ccgcgccttg ggagtcccaa tagccgtttg tactgatgga ccagtcggtg
    26281 taggcaaacg tcaggccgcg gaaggtgatg tcgctgacgt gctttcctcc tttttccccc
    26341 cggatcacga ctgcgttttc caggcacgag gcgatggttt ccgcctcgtc aggagtctcg
    26401 ccctgttccg gtatgtaggc gatttcgccc gtctcgcggt cgtggtacca ctgtcccgga
    26461 cgtttcaacc cttcccggat gttgaccaag cggtagtaaa tatccgtgcg gattgaagag
    26521 ttgaaaagct tgtcggcatc cagagaaagc acggcttccc ttttcgcggc atcaatggat
    26581 gaaacgtggg cgcgggcata ttgccacgcc caaatcatgc tcacctccac gtctcggaaa
    26641 tggtgccacg agggctggat gtccccgcgg gcgaagacaa accccttcct gctgtcgcgc
    26701 gtattggtgt cgccgggaag attcgcctcg ggcacgaagt agccgttttc cggaaaagcc
    26761 gggcgcagtc tgcgtcgacc gttcacaaaa aactgcgaaa aggctctttc ccttcgtgcg
    26821 gtacccggcg cagtcgcgcg ccagacaccg tcggcgtccg ttttccatcc ggtgatccgg
    26881 cgtcccgcac tgaggacggg tttcgccccg tccgcagcca tgaaaagaag cggggcctcc
    26941 ggcgtacccg cgtcctgcgt acggaaggcg agcggctctt ccaactcgta cacaccctcg
    27001 cggatttcga ccactacggg taccgcacgc cccggttccc tcattcgcaa tgcccgtacg
    27061 gcgtcacgcg cccgtgaaaa cgtcgcgaac ggcgctgccg ctgttccctc cgcccggtca
    27121 ctgccgttgg ttgcgatcgt gaattccgcg gccgcggccg tgcccgccac aacggcggcc
    27181 accgcgatgg tcatcttgcc ggtaaaacgc atccgttctc cctttccccg tgtttgttgg
    27241 gcttcctttg tgatttcatt gtgatcataa ctgtggaggg atgattacgt cgagcggaaa
    27301 agcggaacgg cggacggcaa aaaaaagcga tctgtgtttg actccggaga ggggatcggg
    27361 tagtctacat gaccatacgt gatctttatc agttgtatcc ggaaggaagt gccgcaatga
    27421 gtgtcgttca accattgctc gacctgcagg agattgatga ccgcatccgg aaactccagc
    27481 aggaaatcca tgacattccg cagcgtaagg cccaggagca ggagcgtctt accagcgttc
    27541 aggaagttct gacccgagcg caggcggagt taaaggccgc ccaagtgagg gtcagcgagg
    27601 cggagctgga ggtcaaggcc cgtcaggaca agatccagtc gatcaaacag aaccaggtct
    27661 tgttgaagac aaacaaggaa ttccaattgt ataatctcga gatcgccaaa ctcgaggggg
    27721 agatcgaagc gtacgaggcg cgccaactgg cggcgatgga cgatgtgatc ccggtccggc
    27781 atcgtgtggc cgaggcagag gcgaagttga aagaggatca ggcggccgtg gatgcggatc
    27841 tgacggagtt ggacgcccgg ttggccgcag tgcgcgagac gttggcagcg gaggagggcg
    27901 cgcgggtcga ggcggccaaa cgggtcacgc cgcaatttct gctctattac gagcgtctcc
    27961 gcaccaagcg ctggccagtg gtggtggcgt tggacgaggg cggtgtgtgc aaagggtgca
    28021 atctggtcca gcctccctcc gtggggcaga tggtccggcg caatcagggg atcgttccct
    28081 gtcagatgtg cgggcgcgtt ttgtatcagg attgatccgg aaagaaagcg gatgcgtgcc
    28141 gcgccgctgc gggcggcata ttgcgatttg cggtgggtgc aaggggagtg accggtcgct
    28201 cctgccatgt gcaggggagg aaagtccgga ctccgcaggg cgagaggtcc ggccgtgaag
    28261 gccggagagg cactgccaga tcggtgccga cggaaagtgc cacagaaaac aaaccgtccc
    28321 gtttcggcgg gataagggtg aaaaggtggt gtaagagacc accggacgga cgcggaagcg
    28381 ccgtcgcagg gcaaaccccc tctggagcaa gatcaaatag gggactcgag acgcggcccg
    28441 tgtcgttccg gttcgccgga tagagttccg ggtagattgc tcagatgaat gatcggagcc
    28501 gctttcgggc ggtgaacaga atccggctta ttcgcaccct tgtgcccgcc gcactcttac
    28561 cctatacccg tgccacacct gtgaacaaaa ggttgcgggc tccgtggggc atccccaccc
    28621 gtaacgctag atcagtcgtc ctgccaacga acccaaacgc cgcaagtcga atgtcgcggg
    28681 tcaactctct ttggtttctg gtttcatggt ttcaaggttt catggtttca tccaaatcgg
    28741 ggtcgggatc gctatcgcta tcgggtttca aggtttctgg tttcaaggtt tcatggtttc
    28801 atccaaatcg gggtcgggat cgctatcggg atcgggatcg atcatttaac cacggaatac
    28861 caccacctca cagcctaaca gcctcacagt ctcacagcct ttgagattgc gggcttcgcc
    28921 cgcatctcaa tacggtgcgg cggggagcgc atcgtggccg aggatcgcgt cgagtaagtc
    28981 cgagacatcg tcggctgaaa ccgggcgcgg gtttgcagcg gttgagccgg aggccagggt
    29041 ttcacggatg accgcctcgc gatccggaag cgacttgccg acaaaggggt ttgccagtga
    29101 catcgtctcc agccacgtcg cggtctggtt ctcgatatcc agcccgaggc ggtcacgcaa
    29161 atcgcgcagc gtgtcgctga gcaccggacg gttaaaggcg aggcaggccg acaggcaaca
    29221 ggcgcaggcg aggccgtggg ccgcatggaa gcgtacgccc aacggatggg ccaggccgtg
    29281 caccacgccg agccgcgcgt gggagagtgc cacacccgcc agaaaactgg cctccagcat
    29341 ggcggcggcc cggtcggcgt cgccgcgata caaggggagc agattttgtg cgatacgcgt
    29401 gagggcgagt tcagagagta cccgcgtcca gggggtggcg tgccgactgg tatacgattc
    29461 gaacgcttgc acaaaggcgt ccagacccgc cgcggccaac gtgtcgggcg ggcagtcccg
    29521 cagtagccgc ggatcgagga tcaccagacg cggcaggtag gagggatggc ggatcgattg
    29581 tttgagtgtg cgttccggat cggtcagcac ggcgactcca gtcgcctccg atccggtccc
    29641 cgcagtagtg ggcgctgcga tcagcggcag gggtgcgggc gggatgagcg aggcgtggct
    29701 catgtagaac gtcacgcttt ctggcgcgtc ggccagtccc gcggccgctt tggcgaggtc
    29761 catgacgctg ccgccgccga cagccgccac ccagtcggga cgttccgtgc ggatcgcggt
    29821 acgtaaggct tccaccgcat ggacggtcgg ctcgccgccc gcgtggcacc aggtgtgcac
    29881 ggtcatgcca gacggcggtt gcgccaaaat tgcgtccagc aggccggagc gctggagtga
    29941 agcgccgtgc acgagcaatc cgcgcgggcc gaacccggcg gcttcctgcg tgagatgcaa
    30001 tatcttgtcc ggcccggcaa gggtccggtt cggatgcaac agggtagggg gaatcaggtt
    30061 catggttttt aagcgtaacg gaatcgcggc cgaatgggga gcgaaaaaag atgcggatga
    30121 aaggtgaaat caagattaga ttgccggatt cagagggaaa cgatagcata cttttctttt
    30181 catgaaggtt tgaggcggaa cgtgcaacga tccgccgggg cagggcaaaa gcatatgagc
    30241 agattacgat tttggaacga gcgggcggaa accctgtctc gggacgagtt gacggctgtg
    30301 caactcgcgg gactgaaatg gaccgtcaac cacgtttgga ccaacaatgc cttttaccgg
    30361 gaccggctcg ccgcgcatgg catcgagccc ggagatatcc gttcgctgga tgatctccgg
    30421 tccctgccct tcctgaccaa agacgacttc cgcgatcagt atccgctggg tatgctctgc
    30481 atgccgcaag ataccattcg ggagatgcac atgagttcag gttccaccgg cacgccggtt
    30541 gtgatggcgt acaccgaggc cgatctgacg caatgggcgg agtgcatggc gcggtgtcag
    30601 gtgatggctg gccttcagcc aggagacgcg attcagatca tgccgacctt cggtctgttc
    30661 aacggcggat tcggctttta tcacggtgcg cggctcgcgg ggatgttcgc cgtgcccgcc
    30721 agttccggca atacggcgcg ccagatcatg ctgctgcgcg acttcaaggt gcgggccatg
    30781 ggttcggtcg tctcgtatgc ggcgcgtatc atggagatac tggatgcgaa acagatcgaa
    30841 ctgccccatt tgaagatcgg gattttcggg gcggagtcgt tttccgacga gatgcgcaac
    30901 cgtgtggagt cacggctcgg aatccgcgca ttcgacatct atggaatgac cgagacgggc
    30961 ggggtgggga ccaccgggat ggattgcgag gcgcgctgcg gcatccacgt ctgggaggat
    31021 cactacctca cggaggtcgt ggaccctcag actggcgcgc ccgtgccgga cggagcggtg
    31081 ggcgaattgg tgttcacctc gctgacacgc gaagcggttc cggtgatccg ctaccgtacg
    31141 cgggatctat cgcgcatcgt cagccgggag ccctgcgcct gcggccgcac gagtcttcgc
    31201 atcgaccgga tcacgggacg gtgtgacgac atgctgatca tcaaaggcgt caatttcttc
    31261 ccgaagcagg ttgaacaggc actgatggaa atcccggggg tgttaccgga ataccagatc
    31321 attctggagg agtgccacgg ggtgacggat gtgcgcatca acgttgaggt ggaggagggg
    31381 gtcaccggtt atcaggtggt gaaacacctc aagaagcggc tcggattcag tccgaaaggt
    31441 gatgtcttta aacccggcac gctgccgcgc ggcgaaggca aggccaagcg cgtgtttcgc
    31501 gaggttcgcg gttgagaaaa cggcgggccg caggggttct gccagtctgc cgcacgggat
    31561 gggaagagac aaggaatcat atgaacgttc aacgctcaaa cgttgtcggc taagagggaa
    31621 catgcgctat ctaacctacg tgggttgtta tacggatgag aaaaaagagg gcatccacat
    31681 cttcgagacg gacagcgaaa ccggcgcgtt ttgtccggtc ggtctggtgg gcggcatcga
    31741 gaacgccatc cacctgacgc tgaatgccag ccggacccgc ctgtacgccg ggctcggtat
    31801 gccggagtac ggggcgtccg cgcaaaacgg cgctgttgcg gcgtatgagg tgcagggtga
    31861 tacgctccgg ctgctcaacc attgtccggt aggggtgacg ccaccctgtt atgtggcact
    31921 cgatccttcg gaacaggcgt tggcttttgc agagtacacg aatgcggtgg ccggggtgtt
    31981 cgaattagct gaagacggcc ggttcaccga tgcgccggcc gtgacagtgc agcacgaggg
    32041 cgaaggtccg agcaaacccc ggcaggacaa ggcacacgcg cattgcgcgg aagtgacacc
    32101 ggacggacgg tatctctgcg tggtagatct gggccttgat caagtgcggg tatacgactt
    32161 ccctgaacgg cggggcggat tgcgcgagcg atctacgctg accatccgca gcgtgggcgg
    32221 agccggaccg cgccacatcc tgttccatcc caacggacgt ttcgcgttcg tcctgcacga
    32281 gttgaacaac acgatctctt cgttccggta tacgggggag gagtttgttt tggtgcagac
    32341 cgtcccgatg cttccgccgg agttcaaggc gtacagtaag gcggcggcga tcaaactctc
    32401 cgcggacgga cgtcagttgt tcggttcgaa ccgcgggcat gacagcatcg cggcgtatga
    32461 tctcgatcct gacagtgggc gcatgtcctt gctggcgatc tccaaactgg tgggcgaatt
    32521 tccccgtgat ttcgagtttc tgcccggcgg aaaattcgtt ttactgggcc atgagaattc
    32581 gaacgagttg ttttcctatg cctacgacgc gaagagcggc gtcttcacac cggcacacgg
    32641 cccgttcagt ctccatcgtc cggtgctagt ccgattcgga gtccgccgta gctgacgcgg
    32701 ggcgcgtccc tgttgtttta acgcattgcg tccgggggga atgctcaagg ggaaccatga
    32761 aacacatgga acttcatgaa accaggcccc cccccccttg gcgtttctgg cgtcttggcg
    32821 gtttgctcaa atctgagccg gttctgccga gtgtaaccta ttacttctta cctattacct
    32881 cttacctatt acctcttacc tattacctct tacctattac ctcttccttc cgcgatcagg
    32941 gtttagaggc gaaggagatc acgcagaagg agacgacgag cactgccgtg ccgaatgcga
    33001 gataggcctt ggtcttggcg cttgttcctt tccattcgcc cgtcatcacg ccgatgatcg
    33061 tggagaagag gatcgtcgaa gccatcatga tcgcgaagga gatgtacttg aggtcgccca
    33121 tcaggggctc gccggctttg gtgcagacga actgcatgac ccagatgatc ccgatcgccg
    33181 cgcagaggag gatgttgcgg agcgaaggct tcgcatagtc gccgaaggtc ttgttcttcc
    33241 agttctgctg gaagcaccac gccgcttcga cgatgaaccc gccccagagt acgaccataa
    33301 tgaccggcat gccgcgccag caggggttcg cccccgcctt gactgcggct tcttcgatca
    33361 cgcccgcgcc ctgcaggccg aagttcatcc ctgcggagaa gatgcccgcg atgatcgcgg
    33421 tgataatgcc cttcttgaag tcgaactcca tgaccgcctt ctttttctcc tcttcgggca
    33481 gctcgtcttc tttgagcttg cccgcgagac cgacaaagac aatgccggcc agagagccga
    33541 acacaccgcc gagcaccgta agcgccgcag cgtctttgac aagatcggcg gcgtgaccgg
    33601 tcgcgatcgg cgggatgagc gttccggtgg cggcacagag gccgcagccg atcgccagcc
    33661 cgagcccgat gccgaggtag cgcaccatga gcccccaagc cagcgcgccg acgccccaca
    33721 tcatgccgaa gccggcgcag cgcagcagca ccgcattcgg ggcggacatg acgacgttcc
    33781 agaagtcggg aacggtgaaa gtgcagatca ccggcggaca gatgatcagt ccgataaaga
    33841 cgtatacgaa ccaccacgat tcatacgccc accccttcac atagcgggcg ggcagcagaa
    33901 acgtcgcacc cgcgagaccg ccgcaggtga agataagcat gccgagtatt ggattcattt
    33961 tgtgccgcct tccttagccg tcgagaaagc tttggcgatc tcggcttttg tcatgggtgt
    34021 ttcaagatag cgcatcttat ctgccttgag cgttttcggg ccggcccagt ccaacgtgaa
    34081 ctcgccttcg ccgcgcgtat gccagtaggc gacgacgcgc ttgccgtcac gctcgaagac
    34141 aaacccgcgc aacaatttcg cctcagcgct ggccggaagc atctcgatct cgtaaagcgt
    34201 gtacgcgccc ttgtcatcaa gatacaggtg gaactcgcgt ttcgaatcct tgagcgcttt
    34261 ccgttgcgca tcggtgagcc acttcttcgc acggacatcc tcccagcggc gcatgacctc
    34321 cagaaggtcc gccatgcgcg ggtgcttttc gagggccggc aagttgaact ggatcgttgc
    34381 gggacaatcc caagccgcgc cctttgacgt gccaaactcc cacatgtcag gctgcgtgcc
    34441 gacagatttg tccgacggct ggtatatgcc ccaccaaccg aaattgagtc ggctgaaatc
    34501 ttgccgcatg atcggagcgg cgtaaagcgg ccaacggaca atcatatcct taaaaatttc
    34561 tggcgggaaa acgtcgaagg cattcgcgcc gctcaggtga tgccagccga agtgcgcctt
    34621 ggctgcgccc tccataaaga gcggcttctt gtcgaacttt ttccaaacgc ggtattgggc
    34681 gttggggacg tggatgccct gaggaacgtt gacgccctcc gagccgtcgc agtacatgaa
    34741 ctcgaaaccg cagttataga tttttgcgat cttttccgcg acctcatctt gcaggtcggt
    34801 gttctggtca atgtaacaac ttacaccgcc gaactcgcag acatcgagaa tgccgccgat
    34861 ctgcccgttc ggatgctcga cgatggttgt ttcctttgca ccacgcttca cgccggtgaa
    34921 cttgtagggg cgctcggttg tgaatccttc gtagcttatg agttctccgc caaaggcgag
    34981 gatacggctc gcttcgttgg tcgggcaatt cgacggatct tcatgcaccc agacatcgcc
    35041 gtcctctttg ccgaggggtt tcgccagcgt gaaatgcgtc gtcaggttga gccgcggatc
    35101 ggcgacaggc gtgacataac gcgtcttgaa accgataaag gtttgcaaaa cgtgcagtcc
    35161 cggcgtgatg cccgccgcct tgatttttgc cagcatctct ttgagcgaat cgtaaccgtt
    35221 cacgtactcg tccagcaact cgtagtcggc gatgccgccg taaccgccgt cgcccttggc
    35281 gcccttgcag atcgcgggat aataaatcag catgttgcgg aagccgccct gtttcgcgta
    35341 ggcgaggtgc cggtcgaccg tggacggccg cacgccggaa ctccagtaga tcgacgactt
    35401 gatttcttgc gagcggcggc tttggacgcc gcggggaagc cccagatcgc gctcaaaggc
    35461 atccatggca tccatgaagt tgcccgttgc agcaacgacc agcgctgttt tcgcgccgcg
    35521 cagtttcagg ttgcggtggg cttcggcggt catcagccgg aagccgtggc gtcgtgagtt
    35581 gtcgatccag atgtacggtt cggcggcgac aaccgcgacc gccgcagcat cgtcccaact
    35641 gacgttgagc cagccgccga agtatttgcg gttcttgacc ggcagatcca agatcttcat
    35701 gacgtccacc ggcgggtagg tcatcttgag cccgttcgat cccctcgggc cgagcgggaa
    35761 gtccacaagc tcgaacgtca cataatcggc ggcctcggtg actttgagct gtgcctcgta
    35821 ggagatgagt tcaaagccga tgtagaggaa atcgcccgcg cggcgcacgc gatccgcgcg
    35881 gtactcggtg cgtttgttcg ggaatgtgag cttcacctcg ttgttgaacg gacggttctg
    35941 gatgaccgcg aaggccctga tatcctcgtt cagatcgaga agctcctcgc ccgtcgcttt
    36001 gaggacgagc gacttcgcct tggcgtcgtc gccgaggaca aagcggaagc gggggttctc
    36061 gatgacgaca tcagcaaagg cgccgatggc ggcgaacgct gccacggcaa gaacgacaca
    36121 ggtttttttc atgattaaag tattcccgtc tcaacatgga atgtgactga ctcgccggga
    36181 gcgagggtgc gctgttggtt gtgcgcctga cagtagagcc gtcccttgtg ttcgcaagtc
    36241 gccgggaggc acatgcccat cgcgtcctca tccttcgtgc gcgcaatcca gcgcgtcgcg
    36301 taggggagtt cctctgggcg gtagcgcacg aacacgccgc cgcctttcgg atacacctgc
    36361 ttcacgaacg cccagccgtc cttgtcgggc ttgtggaagt agcagttcac gatttccggc
    36421 ttgtaggact cgcctttcgc gccgacgatg gactgcgcct tgtagtcctt gtccagcacg
    36481 gccagccact tgttcgttgc gtttgcccac ggcttgtgat agtccgccgg tacttcgtgg
    36541 ttgatgatcg gctttttcgc aagcggggat tcgacgatct tcgaaccgtt gaccggacgg
    36601 tagttgatgt ggcagaggta gtagtactcc agcggcagat ccttgaggtt ctttgtcgtc
    36661 acaaagatat cgagcttcgc cgctcccttg cggagaacaa cgcgcggtga aaactcgtag
    36721 ttgtaggtga agcacatgtc atggaacgcc ttgccgccga cagcgacgta ttcgccaagt
    36781 tcgtcactgc cgaactcgat gtacgcctcc ttgtagcgga tgatcggcaa ttccgcgtgg
    36841 ccgacgtgcg tgtcttccgc agtcgggttg cccatcgcgg tgagcccgca gtgcatcatg
    36901 aagcaaccgt acgactcgtt gaagcacttg acgcaatcct tgggctcctc gtacatggac
    36961 ttcatggtga gttcgcgtcc gtcgaagtca caccgccaga tcatctgtcc catgaagggc
    37021 aggatcgtga acgaaccgcg cgagttggac actttgagcg catcgacgcc ggtcgagtag
    37081 cggaaggtct cggccttaaa ctctccgctc tcggcaagtg tgcgcttcac ctcggaaaag
    37141 aactcattac gaaggttgat tttcattttc aggtactcac tttaagatgc ggcggaagtg
    37201 atcgaggatc atcttgacgc cattgtcac
//
LOCUS       DAOSLE010000012        34213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1457_length_34213_cov_7.042772, whole genome shotgun sequence.
ACCESSION   DAOSLE010000012 DAOSLE010000000
VERSION     DAOSLE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 34213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34213
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_34213_cov_7.042772"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..675
                     /locus_tag="PK576_01835"
     CDS             175..675
                     /locus_tag="PK576_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HOE60362.1"
                     /translation="MSDLCPCGSGIEFESCCEPFLLERQKPLTAQALMRSRYCAYAMG
                     DIDYLYQTSGAKVRKEFDAESSRNWAKNAKWTGMEIVSLEGGGENDDTGVVEFIAHYS
                     VKETDFNHHERAEFGRVGGEWRFLDGKIFGPDPVRRDHPKIGRNDPCPCGSGKKYKKC
                     CSQTVA"
     gene            651..1346
                     /locus_tag="PK576_01840"
     CDS             651..1346
                     /locus_tag="PK576_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE60363.1"
                     /translation="MLQPNRRMTLQAVLFDLDGTLVDSERLWAEAMRDLLVDRQCPCD
                     TETVLRIVYGHSWIDIYQALVTRFPVLSEIPRHRLAEHLRVYYERLRGQGEGVVIRSS
                     VTLLKQLARDYPVILVSGSPRHDLEEAVRLMEIERDVRFVLGAEDYEPGKPSPAGFLL
                     GARRLGVPPEHCLVFEDSRVGVLAAKRAGMLCVALARPGSHPQELSEADWILEDLAAF
                     TVDGLRKRLARPK"
     gene            1501..1576
                     /locus_tag="PK576_01845"
     tRNA            1501..1576
                     /locus_tag="PK576_01845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1534..1536,aa:Gly,seq:gcc)
     gene            1587..1662
                     /locus_tag="PK576_01850"
     tRNA            1587..1662
                     /locus_tag="PK576_01850"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1620..1622,aa:Lys,seq:ctt)
     gene            complement(1701..2333)
                     /locus_tag="PK576_01855"
     CDS             complement(1701..2333)
                     /locus_tag="PK576_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOE60364.1"
                     /translation="MCERQSYPVVEIFESLQGEGYNTGLESVFLRFGGCNLKCPWCDT
                     RHETFETLDEETVVARVTACSARAVILTGGEPFLQTGLEGLLTRFRDAGFWIGAETNG
                     VLAPSAEVLRRVDYLTVSPKALYAPLYDDARMVRRADEVRIVVDGEVRDFCMAMRNRI
                     EAPRYYLSPCERDGTFNIGETVRLLGELNRGRRSGKWRLSLQTHKLAGFR"
     gene            complement(2340..2750)
                     /gene="queD"
                     /locus_tag="PK576_01860"
     CDS             complement(2340..2750)
                     /gene="queD"
                     /locus_tag="PK576_01860"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOE60365.1"
                     /translation="MLTVSKQCQFDAAHVLPHHAGQCKNLHGHTYRVIVEVAEDADSE
                     DMVIDFKDLKQVIRDVIVDRFDHAFLYDETSESECEIAAVIAKHGMRSVGLPFRSTAE
                     NLARYFFHELATRVNVVSVKVYETPESCAEYRRG"
     gene            complement(2803..3471)
                     /gene="queC"
                     /locus_tag="PK576_01865"
     CDS             complement(2803..3471)
                     /gene="queC"
                     /locus_tag="PK576_01865"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOE60366.1"
                     /translation="MRKNKAVVIFSGGQDSTTCLAQAIRDYGEGNVSCITFQYGQKHS
                     REVEVAKEIARIFRISDHPILALDWYRQVTTNALLDSDMAIAAGQGGACPNTVVDGRN
                     MLFLLVAAIYAKSRNIRDLIIGVGETDYSGYPDCRDVFVKSCNETLNLAMAYPFRVIT
                     PLMHMTKAETWALADKLGILELVAEKTLTCYNGVVGRGCGTCPGCLLRQRGYEAYLAR
                     RAHG"
     gene            complement(3789..5021)
                     /locus_tag="PK576_01870"
     CDS             complement(3789..5021)
                     /locus_tag="PK576_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE60367.1"
                     /translation="MTRQIGFTDTVVRGVRVLLLAALWCAGVGARAAEPTTAPFAGKI
                     RLLLPPRLYAVPGIEMNVYFDNVCLMPNPESVVFDVTCTKGRQQVERWTFVPEEEDAG
                     EYPFVLEVRDAANTIIARAESRLRVTPRAAGAGAPVTVLTVGDSLTHATVYPAHLLEL
                     CKAEGNPHVTLVGHTPYTNRPAIRIEGYGGWTAKQFTTGYKEGKRDTGSQDWRVWNAS
                     SSPFLYADAQGVPALDFARYCREFNGGRAPDFVTFLLGCNDLFGADDGSIDAILDAMF
                     KHYDALIEMVRGVSRETRIGVLLVTPPAGTQDAFGANYRCGLTRWQFKRNQHRTVERM
                     MERYGGREGENLFLVPVHVNLDCVHNYPTLQGPWNARTREAGSRLNNGVHPSREGYLQ
                     IGDSLFCWIKAQLVADNF"
     gene            complement(5080..5556)
                     /gene="queF"
                     /locus_tag="PK576_01875"
     CDS             complement(5080..5556)
                     /gene="queF"
                     /locus_tag="PK576_01875"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009646470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOE60368.1"
                     /translation="MRRTKKTVPGVTRLGSQGTVYADQYDPSVLEAFANQHRDRDYWV
                     TFTCPEFTSLCPVTGQPDFGTIIIRYVPDQRMVESKSLKLYLFSFRNHGDFHEDCVNV
                     ILNDLKALMDPKYIEVWGRFAPRGGIAIEPYVNFGRPDSAFAALAETRFATHPAAR"
     gene            complement(5570..7912)
                     /locus_tag="PK576_01880"
     CDS             complement(5570..7912)
                     /locus_tag="PK576_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE60369.1"
                     /translation="MGEGRERMRVSANRKPGELIAALQAVQDACGYVPPEELARIAVT
                     MGVSEAELTGVVTFYDQFRLRPVGRHRIRVCVGTACHVKGAERTVEAFRRALRIPEGS
                     DTDAEGEFTVERVACLGCCMLAVAVQVDGVIYGQVTPARVKAVLRDVRAGETQIRDTA
                     DVPHPQTGARVRLCACSSCAAAGAADVLHEIRRVVRTWRLPVGVREVGCTGGSYHAPL
                     MDILLEDGLHFRYGRVRVRDVERILLSHIRPARTAARLQATLRAWTEQLFAGEEDDPP
                     VRFPLSVRDQSDAGYWLCQHRIATEGAGERSSLDWDEYCRHGGFEAYRTCARQMTPQE
                     VVAQLEASGLRGRGGGGFGTGRKWRAVADAAQGSDAVVVCNGDEGDPGAFMDRMLLES
                     YPFRVIEGMMIAAHAVGASEGIFYIRHEYPLAVRRVREALALLEREGILGAEVPLRLR
                     VVEGAGAFVCGEETALLSSVEGRRGVPHVRPPYPSESGLFGRPTLVNNVETLALVPWI
                     VRHGAAAFRAVGTAGSPGTKAFALAGKVARGGLVEVPMGLPLRQVVEQIGGGVGEGHR
                     FKAVQVGGPSGGCIPADLSDMPVDYETLTRAGGMMGSGGMVVLDETDCMVDIARYFLS
                     FTQRESCGTCTGCRIGTRRMLEILERLCNGEGRPEDPAALERLAESVRAGSLCGLGRT
                     APNPVLSTLRHFRAEVEAHVRGVCPARACRALIAYTVSDRCIGCTLCAQNCPAEAIAF
                     TPLERAVILQDRCTKCGSCRQVCPEKAIEIEDAKRDNEIR"
     gene            complement(7966..8775)
                     /gene="truA"
                     /locus_tag="PK576_01885"
     CDS             complement(7966..8775)
                     /gene="truA"
                     /locus_tag="PK576_01885"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE60370.1"
                     /translation="MAANGQRKPGGGPTAATLPGPVARRFRLTVAYDGTAYAGWQVQP
                     HHPSVQQTLEEALAGIVGHTVKVHGSGRTDQGVHARGQVAHVDLVCRMTPVAVGRALN
                     ARLPQDIRIRSVARVRPDFHARRSAVGKEYRYFVWNGAQLLPDRRLYAAHVFRPLDVE
                     AMRQAAAFFAGQHDFASFTANPQREVPSTVRRVFDVTVGKRGREIVFRVRGEGFLYKQ
                     VRSMVGFLLRVGEGAEPPEAVRDLLASRAPRKARVPSAPARGLFLWRVWYA"
     gene            complement(8803..9885)
                     /locus_tag="PK576_01890"
     CDS             complement(8803..9885)
                     /locus_tag="PK576_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOE60371.1"
                     /translation="MRHAYAVILAGGSGERFWPLSTKARPKQFISLFGGRPLLALAVE
                     RLSGLIPADRILIITAEHLVEQTVEAAWHVPHANIIGEPCKRDTAPAVAVACGLVRQR
                     DPEGVVCILTADQLMSDVETFRVTLADAIRVAGCEEAIVTIGIKPDTPATGFGYIEVG
                     EAVETRTVTLFNRAKRFVEKPDAYHAARYVESGTYFWNAGMFIWKCDVMREALERFAP
                     DLAELCDAVVAAAPGPELANLLQRVYPSLRAISIDYAVMEHAPNILVARGEFGWDDVG
                     SWPAVAGHFSADLAGNVVVGSCEPMESLNNIVVSENRLTALIGVSNLVVVQSDNATLI
                     CPQDRAEDVKKLLRRIAQRPDGEKYV"
     gene            10083..11441
                     /locus_tag="PK576_01895"
     CDS             10083..11441
                     /locus_tag="PK576_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60372.1"
                     /translation="MNRKRDALISMTVAMGVAACCFAEFESGCPEYAPELKDRCWMWG
                     HKTGQYDQPNGWFRIPVSPDVTMAEACRYMGIPNVSIVIWHIPDEKFMSEFTDMKRVA
                     WVVDNGDLHLHGRDILPEFLAFGEKKMKTMPNLMGFEFDDFFDQPRKPVRKEKLADGT
                     EVNVLPGSRTLEELRALRKRLHSYDRPIDMRLVFYERDLDHPEETKAVVDIFDTVMFW
                     TWFGKNVQALENNFKAYRKIAPKKPTLLGIYMWDFGGSQPLDATSMKLQLDFALKLFR
                     AGEVEGFIFHCTPLVNKNIEAVELARRWIIEHGNEKHGGGEQRVPPIPEPPALRPEQT
                     MTDFGRLAFEEGKKPNGALLLTGPGKIRIVERTGRIAWENVAQANPEKAFYHGGHVYF
                     EEDGIQKRIRYSLPCDRLAKAEVVPEAQRAQVPKNKKFDSALLDCLKDRSGYSGAMNL
                     QR"
     gene            11662..11925
                     /gene="rpsT"
                     /locus_tag="PK576_01900"
     CDS             11662..11925
                     /gene="rpsT"
                     /locus_tag="PK576_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOE60373.1"
                     /translation="MANIKSAIKRAKQSEARNVRNRAVKSRVLTARKKVLAAISAGEK
                     EAAQKLYSDYTSVLDKAAKKGVIPKNTASRKKSRVALSMQKMA"
     gene            12013..12669
                     /gene="lipB"
                     /locus_tag="PK576_01905"
     CDS             12013..12669
                     /gene="lipB"
                     /locus_tag="PK576_01905"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOE60374.1"
                     /translation="MRELEVSDLGRDAGYNEVLALQERLHTRRAAREIPDTLLLLEHR
                     PVYTLGRSAAAAHVLYSEERLKEAGIERVQTSRGGDVTYHGPGQLVGYPILHLGDAGL
                     RVLEYVTALEETLIRAVADFGIVAARDPRNRGVWVGNAKLAALGIRVAHQVTMHGFAL
                     NVAPRLDDYRGIVACGLTDASVTSLQHLLGEAPDMPAVKQSVVRAFREVFGYDRNITA
                     "
     gene            complement(12697..13131)
                     /locus_tag="PK576_01910"
     CDS             complement(12697..13131)
                     /locus_tag="PK576_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60375.1"
                     /translation="MKGRDISFREVVQGICAKDPRYDEDAYLFLMEALDSTVKAIREK
                     EPEHGRDVSGRELLEGIRAFALDEYGPLAYTVFAEWGIHETADFGAIVFNLVEAGRLG
                     KTESDSLEDFNQVYDFDEAFRLPFEPQEPPEKDGAAGKRQRG"
     gene            complement(13128..13547)
                     /locus_tag="PK576_01915"
     CDS             complement(13128..13547)
                     /locus_tag="PK576_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOE60376.1"
                     /translation="MTDPECVFCRIIAGQIPAKVLFENEEAIAFLDIAPFEPGHALVV
                     PKMHAALFTELPDSVLSHLMPLVKRVASLLLERLPCDGFNILQNNGRCATQVVPHVHF
                     HVVPRWNGRAINWISGAYASAEEMESVYRKLTVAAGV"
     gene            complement(13668..14615)
                     /gene="ispH"
                     /locus_tag="PK576_01920"
     CDS             complement(13668..14615)
                     /gene="ispH"
                     /locus_tag="PK576_01920"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOE60377.1"
                     /translation="MCNRRKLTVVTPHGFCSGVARAVATAEAVLAACPGERVYCLNEI
                     VHNRQVVSRLTDAGMVFVSDPAEVPQGARLLFSAHGVSPEVRRQAAARGARVIDATCP
                     FVMKVHHEVVRFAAQGACVVCIGHRHHEEVVGVAGEAPDQVRIVECEAEAESLDVPST
                     QPVAVVTQTTLDAARVEGVMRVLRRRFGARLIVPASTDVCYATRNRQRAVSLLAERCG
                     LVLVLGSENSSNSQRLVETARTAGARACLVSTPSCLAEVAGLADEPAVGLTSGASTPE
                     AFLDEVIRLLCEGYGFPAPEILCAVEEESHVFRLPPLPE"
     gene            complement(14608..14931)
                     /locus_tag="PK576_01925"
     CDS             complement(14608..14931)
                     /locus_tag="PK576_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE60378.1"
                     /translation="MDEWVDIRKDERHVARERAKARVLRNSAWWREQIRPGVCRYCGK
                     TVGADALTLDHVVPVARGGTSTRGNVVPACVACNREKNVLTPAERILTELERDAAAKT
                     GHDDV"
     gene            complement(14947..15720)
                     /locus_tag="PK576_01930"
     CDS             complement(14947..15720)
                     /locus_tag="PK576_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOE60379.1"
                     /translation="MNGPLFFSTFILIFLAELGDKTQLAVMSQSAASSSKWTVFAAGV
                     AALVASTAIGVLAGGLIRRFVPDERYIKCAGGLLFLLFGVLMLREVFGTKTVPVRTAA
                     PPVSAFSDWIGRFVIQQAAVFEKAAFEDYEALAARSLDPEEKALYQRLAQEERWHHQA
                     MLGAMEAGAGRDIPITAEMAGALPPFNEMMHDAVKAPRELEHAIEHERAMSRFYQILA
                     EKSTLPRLRETFRALAAAEANHATRLLERMRNAETKTTE"
     gene            complement(15867..17726)
                     /locus_tag="PK576_01935"
     CDS             complement(15867..17726)
                     /locus_tag="PK576_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HOE60380.1"
                     /translation="MNGENGKRLRIALIYMPFAPPEGPAIGLTQIASVLKQRFGGQVD
                     VRIHYLNLDVAALPDAAGFYGAATSPYGRMTGLADWFFRAAAFPDAEDNIEAYLGRYY
                     YDGATEATEVAEFARARRGELFAFLDELIDRYALDSADLVGFSLCFFQTVASLAMASR
                     LKLRNPEVTVVFGGPAVKGVPGKTLVEHAPCVDAVFSGPGLVSFPELVGRRLAGLPIT
                     RPAIPGVFARGVADALPPACNVGAQLDIRKDVPLDYRSFLDHFDAVLGDSGRQPFLLM
                     QTSRGCWWADRRRCTFCGLNDLQERFEALPPEAALRHIRSVLGYAGRVSRFVACDNVV
                     PPNYFREVFPHLAPPQGVFIKYETRSAITAEEIRVLCRAGIRCVQPGVEALSTESLTL
                     MRKGVTAFDNLRFLKDCLRHALYVEWNLLLFSPGEREEVYRKYERDIPRLVHLQPPEG
                     VFPIEFVRDSRYFEEAGAYGLDLEPHESLRYIYPFAPETLFGLCYRFTDRNADPVWAE
                     QWLDRLGGLIETWQERWACGPEERPFLLLYRDAQSACIHDSRSGGTGRYRISDLSERL
                     LQEMGETPRTISEAAVAAEVEENAVRREIGFLNERQLLFEEDGRFLSLVISPT"
     gene            complement(17787..17966)
                     /locus_tag="PK576_01940"
     CDS             complement(17787..17966)
                     /locus_tag="PK576_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60381.1"
                     /translation="MGVGFANGLITPRRGVPLAACFGRGGYGTLPLVGGAPREDTAER
                     LIRAAGAGLRRACRA"
     gene            complement(17988..19058)
                     /locus_tag="PK576_01945"
     CDS             complement(17988..19058)
                     /locus_tag="PK576_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60382.1"
                     /translation="MNQVRYALVGFGGIAENRIAKEGFACDVSRFRPLKQAVLVGATD
                     VNPKRRKAAQALGLKVYRDLDAVLADPGVDAVFVATNNLTHAPLARWALEAGRPVIVE
                     KPLAPNARDARMLVKLAARKKLSLSVDHMMTHNTFNREARAQVAQGTLGKVSDACFHM
                     QFAYGFDPAEAATWRCNNRAEMGGPVGDVASHCFYMAEFILDSRIRAVRAVYMPKRMA
                     IRVEDGALIQCDLENGITCSVLVSFCDVRGGLGGTLSNLGYEIYGDQAVLRAYGTLFQ
                     LSGHPDEPFAMRLELDAFDRQRRLRVAKPVNIYRGVIEEHAASILSGRPLSGEGGLHN
                     VLICEAAHASAQRGGRTVAVKG"
     gene            complement(19069..21048)
                     /locus_tag="PK576_01950"
     CDS             complement(19069..21048)
                     /locus_tag="PK576_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOE60383.1"
                     /translation="MIPITWLDAWIIVGFLLSVVLVGSWAARYSGQNTESFFLSGRNM
                     PWWLLGVSMVACTFSCDTPNLVTEIVRLNGVAGNWVWWAFLLTGMLTVFVYAGLWRRS
                     GLDTDLGFYELRYSGRSAAFLRGFRAIYLGVFYNLIILGGVSLAAIKIGQILFGLSPH
                     MSLLVSMTGVAIYATLGGLTGSMWADFYQFGVAMTGAVFAAMYAVRSGMADGVTSLGE
                     LFTHPAVVPNLALFRPSSHDLSTLMTVMILPLAVQWWNVWYPGAEPGGGGFVVQRMLS
                     AKNERHAVGGTLLFNVLHYAVRPWPWILVALASMVVFPLTPQPEREAARMWLEAHAAE
                     VRGYAADKAALPDEVREAVRLKRADAAGVGSLARAFPRVDDTYLGHDIAYPGMVSRMP
                     GGWRGLIVASLIAAYMSTVATLLNWGSSYVVQDVYLRFIRSQAPPRHAVRIGRLTMLA
                     MLLVSAWIAFHMTTVKDIFHVLLQVGAGTGLLYILRWFWWRINVWSEVAAMTVSFVVA
                     LFFQFGAAPLGIESAMADAGWFRLMDFTSWKMVSGILFTTVVWVAVTYLTPPVDDRTL
                     MAFCRKIRPGGPGWKRITERIQAAGDEPAGTEPCWNVPAGLVCMLLGCLLVWAALFGV
                     GYLLYDRIALGCVMSGIAAVAAYGVWKQMGRLRLG"
     gene            complement(21140..22441)
                     /locus_tag="PK576_01955"
     CDS             complement(21140..22441)
                     /locus_tag="PK576_01955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE60384.1"
                     /translation="MATMRKAAKRSGSKQAALALKGGAKVWDKGFPMWPSFSEETLKT
                     ALEPLVSGKVNYWTGPVGMKFEAAFAKWLGVRNAISVTNGTAALHTAIAALGIGPGDE
                     VICPSYSFIASSFCVLQAGALPVFTDVGFDHQIDPRQIEEKITERTKGIVVVHLYGVV
                     ADMGPILKIAKKHNLKVIEDCAQCFGGLYKGEKAGTLGDVGCFSFCQSKHFTTGGEGG
                     MVVTNDDDLAWECRSFRDHGYDVKERLNLLEMEGKLMYIHKRVGFNYRMTEIQSQIGL
                     CELKRFDSWNLKNRIRNGRMLKKALEKHPLVLHAPVDTPQRRNAYWWAPFVLDTSKLT
                     CPIKEFIKAIAAEGVPVYSVLWPEMYKERAYLERNGFGVANYPFFDPKARNIDYSRVS
                     CPRARWMTDRTMCFFTHPVYTEEHMQACIDAFKKVAKAYMK"
     gene            complement(22501..23766)
                     /locus_tag="PK576_01960"
     CDS             complement(22501..23766)
                     /locus_tag="PK576_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60385.1"
                     /translation="MNRTLTRRALMKLTGGAAVASASASVAAATYRRSIGANDRIRIG
                     IIGCGSRGRNAHMKGLYNHVKGQPFEIVALADPWRVAREAANGLVREWFGRDAQQFSS
                     YRQLLECRDLDAVTIASCDLHHTAHLEAAARAGKHIYCEKPLAVEFDDLVRATDAARA
                     AGTVIQIGTQIRSLPSMVGARDLFRTGIFGRISRVEECRNAGRPYWYHYLKEDVREAD
                     LDWKEFLHGLPMRPFRADLFSGWYGYYEFSRGPIPNLGSHFIDLVHFITGATFPTSCV
                     CLGRINETWLDAHEFTNPNVIQATWLYPEGFVVSSSNNLANGANNVRKLYGDKGALDF
                     TNWSKPVYDCEGAPLRDGSIRGKNEVPLTEHPDHYLDWLQCMRSGGTPNASLEAGYQH
                     AVAVIMAMKSYETGRKAVYDPVQRKITTV"
     gene            complement(23962..26952)
                     /locus_tag="PK576_01965"
     CDS             complement(23962..26952)
                     /locus_tag="PK576_01965"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60386.1"
                     /translation="MNGRLRGMLLIGVVLGCGAAEVRFDFESGDLQGWRILDGGFGKV
                     VSDRDREHHGGGPYTKEGRFFLSTLESADGQSPRDVFTGILESPVVVLRGPRISLRVG
                     GGKGADVYVALCSLDGRELAHARGGNSQRILERVWTVPEQVGKPVFFRVSDQSEVGWG
                     HITLDSLVCQGIIDEAATEQRFRERRREIARARGGAAADRLRVAINELAERFGARYPS
                     AVLLERLGAAEAAGDEAQLDAVAREALVARNPLMNAQPILFVTRKQYRPDHHNTETMF
                     QTGEINTDKYDTEGALKVLDAASGTVRTLFAPGPGVTVRDPDVSFDGGQILFSMRRGK
                     GDDYHIYAINSDGKGLRQLTSAPGVSDIDPIWLPDGDILFSSSREPKYCMCNRHIMCN
                     LYRMGADGANIHQIGKSTLFEGHASLLPDGRVLYDRWEYVDRNFGDAQGLWVCNPDGT
                     GHALYWGNNTTSPGAVIDARALSQSHLAIAVLGSCHDRPWGALGLIDRSKGVDGLEPV
                     LRTWPVAFTNRIRTAGQNFDSTVGLQIKYEDPYPLDDRHFLCARKTGKGEETGLYYLD
                     SHGNEVLVHVEAPGCFDPMPLAPRPAPPVHPRRRNFDDPNAPGFFYVQNAMIGTHMAG
                     VAPGTIRYLRVVESPEKRNFSVRGWFGQGEQAPGMNWHNFENKRILGTVPVEEDGSAY
                     FEVPGNTFVFFQVLDANGMMVQSMRSGTYVQPGETYGCVGCHENRVGDIPPPATRPKA
                     LRRKPDRLTGWRGAARLFCFQREVQPIFNRRCVSCHDYGNEAGKKLNLAGDRDVIFST
                     SYTDLWVQGALTCVGGGPAEIQQAYSWGSHRSKLIRKVRAGHAGVKLSPEELDRLITW
                     VDLNAPYYPYYESAYPDHPGGRSPLSQDELKRLKDLTGVAISISHGQRQRSQVSFARP
                     EESRILSGLATNSAERAEALALIRAGARRLHERPRADMEGFVASVRDQEREKKYQARL
                     AEERRVYEALRNGGKRYDPGLPRE"
     gene            27262..28302
                     /locus_tag="PK576_01970"
     CDS             27262..28302
                     /locus_tag="PK576_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE60387.1"
                     /translation="MNRFAYLVRRLLLVIPTFLGITMVCFALTRFLPGGPVEIQLMKM
                     RNLGGANDPGSAAVGQVTEEYRQELTRQFGFDRPLPVQYYDWLVRKRMGLLIASYDYP
                     NRTAWQLIASRIPVSLWFGITGFVLSYLICIPLGVAKALRHRQPFDAISSLIVFAGYA
                     IPAFALGMVLKMFFCGTVEGLADLFPLGGFESDLPATAGWGARMADRAWHMALPVTCY
                     VAGNFAVLTLIMKNALLDQIASDYVRTVLAKGATRRRAIWGHAFRNALIPVATGFGSI
                     LGVLFAGSIIIENIFEIPGMGRLSWEALTGRDYAVFLALLALTSLLQLAGNLLSDFCY
                     LLIDPRIHFGDT"
     gene            28299..29900
                     /locus_tag="PK576_01975"
     CDS             28299..29900
                     /locus_tag="PK576_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE60388.1"
                     /translation="MRRFPRYRLSPLTRKRLERFWRIRRARVALILLTALFAVSLCAE
                     WLCNGRPLFLRVNGRTWLPFLQRLTLRDLEGPDAEATRVNFRAFVRSPAFTTNPLNRV
                     VWAPVPYSPGDVVDAGTLSRFRTVHVSVVPEIRVGRLNLLPDGTVIRAEGCEPFFPGE
                     ARVNGVVLDRHWHVPETLRASLDARAAGRPAPAQRFLLTHLRQSDLRARVTLPETAGA
                     AGPPRLMFRQIETPDLPVQVRFRRDDAGTVYPVTHRTWRQIPATCQPEILALASAAFA
                     GTAPTARIPWKDRHATVACGLTEVAWPFPPVRGHWMGIDSAGRDVLVRVLYGMRTAML
                     FGLLLALWAVLFGVVIGAAQGYFGGWVDILGQRLTEIWSALPFLYVMIFIGSALGRSF
                     SLLLLCYGLFNWIGISYYLRAEFLRLRNRTFVEAARCQGLSSARIIFNHILPNALTPL
                     ITLFPFTLIGAIGSLAALDFLGFGLPPLTPSWGELLQQAQQFRWAWWLILFPSLALFT
                     VMLLAVLIGEGLREAFDPRQATKLE"
     gene            29903..30859
                     /locus_tag="PK576_01980"
     CDS             29903..30859
                     /locus_tag="PK576_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60389.1"
                     /translation="MLLEIQNLQIQFESDGQIVRAVEGVSFAIDRGEVLGLVGESGCG
                     KSATAMCIPRLLPAPPARVTGGRVTLDGRDLLAMPLPELRALRGAKIGVILQDAMTSL
                     SPLHRVGRQLEEAVRLHRRVSPAAARETALDWLDRVGIPDPAARARAYPHELSGGMQQ
                     RVMIAMALLLEPDLIIADEPTTALDVTIQAQVLTLMRRLRRANAGMLLITHDMGVVSQ
                     MATRIAVMYAGQIVEQAASEAFFRTPLHPYSIALLEAMPSAATRGKRLKAIPGQVPPA
                     GNFPPGCRFHDRCPVARPECARTPPALRVLEDRSVRCPYAGA"
     gene            31064..32218
                     /locus_tag="PK576_01985"
     CDS             31064..32218
                     /locus_tag="PK576_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60390.1"
                     /translation="MKKRIVIGTALISLAGYASFPPDAVLSAFPRLAGEHDDAPRFRR
                     AIAAAPNGVLAVGKGDYSIATPLVITNRCSLEMHPAARLIATAPMEFVLTWDGHDDYH
                     ALSIFNPDGSIYDNAGLFIRGGDIDGRGLASCLRIGNAHHFTLANITLHNGRKAGLEL
                     SRWNGGHLYELIANNVYCKCTMKGLAGNIGVLVQVHDCHFTDIIVVDYTIGIRATGSS
                     NRFTRCHVWGGTVAPKGMSMREWSDFYAANKKQRSRWNADLEAALLSKGLPEMLAGSI
                     CFDSSSGNLFDGCYADTGEIGFNLPHGGQLVNCGFYNNLLMGLRKSTVIVHKSGRLVI
                     TGCKFNGVTGVEKLYEGPWKDVVWSSSTASGGADMDADATKLRHGTPVQK"
     gene            complement(32259..>34213)
                     /locus_tag="PK576_01990"
     CDS             complement(32259..>34213)
                     /locus_tag="PK576_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60391.1"
                     /translation="VWHDGIATASQSNYRTVQSGMVWNQPITNVCAVFHVVYAYGSGG
                     NAVLGCTKRFAEMGTARDYNDFGRGINSTVDGGGVGTWQNYLLASGVPSHVRNGWLYV
                     DGQEWDLAAKMPAAYSVPYVLEVHPLAPYGRADAFAIQEHAGVTCSGCQRQMECIVYT
                     NELTLTERRQVCAYLMQKYRNATVEYNLSDSRFTNRSNLDALDVTHDVGVDVAAGNVM
                     GFTTLTNSATLMKTGGGTLYADLVDGASLDVREGEVVIRSQELTADVVPSGPFVHLDA
                     TATNRMTLTSKTYLDGATRDEVTHWRDVSGASPVSAQRRTTDDRYKGPYLSYPESLGG
                     RPVVDYGVLTNTSPVDFNYGWGLFHDLVWQETDGYTPMFYTNLTTLGSVFLMIGSQNG
                     GGVPLGGYGTAQWRTATADPAVPFFNSNAPTGCRSGGNWLNGVRTTFTTAGLSGGYDL
                     VSFRDTVGTGCNVQSLMMANARQFSGGGELGEVLIYEEPLSYYTHQVVDAWLNWKWFA
                     RKTVGHAPSAAEDVTLAAGTTLTVEGDMPLTVTRLAGAGTINGSVAFASGAGLEVVIN
                     DNGTITPLTVSGAVDLSDGGTVTLKGDISKLATGSYPLVFCTSAIAGPWNVQYTGTSY
                     LKYFVRIRPDGIYLDVVSPGTLVIFR"
BASE COUNT         6579 a        11662 c         9745 g         6227 t
ORIGIN      
        1 acgcggtttc cgcttttgct ctatccaacc gtccgctttt gaaattccca cgacaacagc
       61 gtatcgattc ggtgttttcg ggattgcggg gaagaggcgc atacgataca gtacacctcc
      121 gttgtgaccg gacatggcca accgagttgt ccccggtctt caggaggctt tccgatgagc
      181 gatttgtgtc cctgcggcag tggcatcgag ttcgagtcct gctgtgaacc gttcctgctc
      241 gagcgtcaaa aaccgctgac cgcccaggct ctcatgcgtt cccgttactg tgcctacgcc
      301 atgggggata tcgactatct ctaccagacc tcaggggcca aggtgcgcaa agaatttgat
      361 gctgaaagca gtcgcaactg ggccaaaaac gccaagtgga ccggcatgga gatcgtgtcg
      421 ctggaaggtg gcggggagaa cgacgacacc ggcgtggtgg agttcatcgc ccattactcc
      481 gtgaaggaga cggatttcaa ccatcacgag cgggcggaat tcggccgggt cgggggcgaa
      541 tggcgttttc tcgacggcaa gattttcggt cccgaccccg tgcgccgtga ccatcccaag
      601 atcgggcgca acgatccctg cccctgcggg agcggcaaga agtataaaaa gtgttgcagc
      661 caaaccgtcg catgaccctt caagctgtcc tctttgatct cgacggcaca ctggtcgatt
      721 ccgaacgtct ctgggccgag gccatgcggg atctgctcgt ggaccggcaa tgcccttgcg
      781 acaccgaaac cgtcctccgt attgtctacg gtcactcgtg gattgacatt taccaggcgt
      841 tggtcacccg tttcccggtg ctgtcggaga ttccgcggca tcggctggcg gagcatctgc
      901 gcgtgtatta cgaacgcttg cgcggtcagg gcgagggagt cgtcatccgt tcctccgtca
      961 cgctcctgaa acaactggct cgcgactatc cggtgatcct cgtctcgggt tccccgcgcc
     1021 acgacttgga agaggccgtg cgcctgatgg agatcgagcg cgatgtgcgg tttgttctgg
     1081 gtgccgaaga ttacgagccc ggaaaaccgt cgcccgccgg cttcctgctc ggcgcgcggc
     1141 gtcttggcgt cccgccggaa cactgtctgg ttttcgagga ttcgcgcgtc ggcgtgctgg
     1201 cagccaaacg ggccggcatg ctctgcgtgg ccctcgctcg ccccggatcg cacccgcaag
     1261 agctttccga agcggactgg attctcgaag atctcgccgc gttcaccgtg gacggtctcc
     1321 gcaaacgcct cgcgcgtccg aaataagccg cgtccgggct gcctgcggac gcgaatttcc
     1381 gtttctctga tctccgtgcg ccaaaccgcg ctgccgtccc gtcaaaaaaa ctgcaaaaat
     1441 cactggtttt gcggttgcat gccccgcacg cttctgataa agtgcgtccg ttcttttgca
     1501 gcgggtgtag ctcaattggt agagcgcgac cttgccaagg tcgaggttgt gggttcgaga
     1561 cccatcactc gctccatgtt ttttatgggg gtgtagctca gttggtagag caagtgactc
     1621 ttaatcactg ggtccacggt tcgagtccgt gcacccctac cataaacacc ggaaggctgg
     1681 tctgacgacc ggccttttgt ttagcggaac cccgccagct tgtgcgtttg gagcgacaga
     1741 cgccatttcc cgctacgccg gccgcggttg agttcgccca acagcctcac cgtctccccg
     1801 atgttgaacg tgccgtcgcg ctcgcacggc gaaaggtagt aacgcggagc ctcgatccgg
     1861 ttgcgcatcg ccatgcagaa atcccgtacc tcgccgtcca ccacgatgcg gacctcgtcc
     1921 gcacggcgca ccatacgcgc gtcatcgtaa agcggcgcgt acagggcttt cggcgacacc
     1981 gtcagataat ccacgcggcg cagcacctcc gccgacggcg ccaacacccc gttggtctcg
     2041 gcaccgatcc agaacccggc atcccggaag cgcgtcagga gcccctccag ccccgtttgc
     2101 agaaacggct cgccaccggt cagaatcacc gcgcgcgcgg aacatgcggt cacccgcgcc
     2161 accaccgtct cctcgtccag cgtttcgaaa gtttcgtgcc gggtgtcgca ccacgggcac
     2221 ttcaggttac agccgccgaa acgcaaaaac acgctctcca ggccggtgtt gtacccctcg
     2281 ccttgcaggg actcgaaaat ctccaccacc ggatagctct ggcgctcaca catacgcggt
     2341 catcccctgc ggtattcggc acacgactcc ggggtctcgt agaccttgac ggagaccacg
     2401 ttgacgcgcg ttgccagttc atggaagaaa taacgggcga gattctcagc ggtcgaccgg
     2461 aacggcaagc ccacgctccg catgccgtgc ttggcgatca ccgccgcaat ctcgcactcg
     2521 ctttcactgg tctcgtcgta aaggaaggca tgatcaaagc gatccacaat cacatcacga
     2581 atcacctgct tgagatcctt gaaatcgatc accatgtctt cgctgtccgc gtcttcggcg
     2641 acttccacga tcacgcggta agtgtggccg tgcagatttt tgcactgtcc cgcatgatga
     2701 ggcagcacgt gcgccgcatc aaactggcac tgcttcgaca cggtcagcat gattaccccc
     2761 taactctttg ccgatcactt ccatcataaa tcctgttaat cctcacccat gcgcccgacg
     2821 cgccaggtag gcctcatacc cgcgctgccg caagagacac cccgggcatg tgccgcatcc
     2881 gcgtcccacc accccgttgt aacaggtcag cgtcttttcc gccaccaact ccagaatccc
     2941 cagcttgtct gccagtgccc acgtctcggc tttcgtcata tgcatcagcg gcgtgatcac
     3001 ccggaacgga tacgccatcg ccagattcag cgtctcattg cacgatttca cgaacacatc
     3061 ccggcagtca ggatatccgc tgtagtccgt ttcgcccacg ccgatgataa ggtcccggat
     3121 gttgcggctc ttggcgtaaa tcgccgcgac cagcaggaag agcatgttgc ggccgtcgac
     3181 caccgtgttt gggcacgcgc ctccctgccc tgccgcaatc gccatatcgg agtccagcaa
     3241 cgcgttggtg gtgacttgcc ggtaccagtc cagagcgagg atcgggtggt cggagatgcg
     3301 aaagatccgc gcgatttcct tggcgacctc cacctcgcgg ctgtgtttct gcccgtactg
     3361 gaaggtgatg caggaaacgt tgccctcccc gtagtcgcgg atcgcctgtg ccagacaggt
     3421 cgtgctgtcc tgcccgcccg aaaaaatcac cactgcctta tttttcctca tcataaccct
     3481 catggaaatc gtcatccgag aaaccacgcc cggaacacat cgcccttcgt agtcggttag
     3541 agacagaggt catgcgagtc gagtgcgtgt tttgtgcgta cgcagtcggg agcgcgggcg
     3601 tccccgcccg cagagagttg agcgttgaaa gttttttaac gcagagacgc ggagacgcag
     3661 aggtttttac gggacggcgt cgtgactgca agcctgaaaa aaagtgtgta tccgcgcaca
     3721 gcgcggaggg aaagcggggc cgccacgccg tgcacggcac aacaatcttg tcaatcacgt
     3781 taatcatgtc aaaaattgtc agcgacgagt tgcgccttga tccagcagaa cagggaatcc
     3841 ccgatctgca gatatccctc ccgcgacgga tggaccccat tgttcagtcg gctgcccgcc
     3901 tcccgggttc tggcattcca cgggccttgc agcgtgggat agttgtgcac gcagtcgaga
     3961 ttgacatgga cggggaccag aaagaggttc tccccctcgc gcccgccgta ccgttccatc
     4021 atccgctcca ccgtccggtg ctggttgcgc ttgaactgcc aacgggtcag gccgcaacgg
     4081 tagttggcac cgaacgcatc ctgcgtacct gcgggcggcg tcaccagcag caccccgata
     4141 cgggtctccc ggctgacccc gcgcaccatc tcgatcaacg catcgtagtg cttgaacatg
     4201 gcgtcgagga tcgcgtcgat actgccgtca tccgcgccga agaggtcatt acaccccagc
     4261 aggaaagtga cgaaatccgg cgcacggccc ccgttgaatt cccggcagta acgggcaaaa
     4321 tccaatgcgg gaacaccctg cgcatccgcg tacagaaacg gactgctgct cgcattccac
     4381 acgcgccagt cttgcgaacc cgtatctctc ttgccctctt tgtaaccggt cgtgaactgt
     4441 ttcgcggtcc acccgccata gccttcgatg cggatcgcgg ggcggttggt atagggcgta
     4501 tgcccgacca gcgtgacgtg cgggttgcct tcggccttac acaactccag cagatgcgca
     4561 ggatagacgg tggcatgcgt caggctgtcg ccaaccgtca acaccgtgac cggcgctccc
     4621 gcgccggccg cgcggggcgt gacccgcaac cgcgattccg cgcgcgcgat aatggtgttc
     4681 gccgcatccc ggacttcgag gacgaacgga tactcacccg catcctcctc ctcgggaacg
     4741 aatgtccagc gctcaacctg ctgccgtccc ttcgtacagg tcacatcgaa cacgacgctc
     4801 tctggattgg gcatcaggca gacgttgtcg aagtaaacgt tcatttcgat gccgggaacc
     4861 gcgtacaggc ggggcggaag caggaggcgg attttccccg cgaacggcgc ggtcgtcggt
     4921 tctgcggccc gcgcacccac tccggcgcac cacagagctg ccaacagcaa cacgcgcaca
     4981 ccgcgaacga ccgtatccgt gaatccaatt tgacgtgtca tggaatcctc tttcttgaac
     5041 aatccgcttt tccggagttg cacacgcgct gtccgaaact caccgcgctg cgggatgagt
     5101 cgcgaaacgg gtttccgcca gcgcggcgaa cgcggaatcg ggccgtccga agttgacgta
     5161 cggttcaatc gcgatcccgc cgcgcggggc gaaccgtccc cacacctcga tgtacttggg
     5221 gtccatcagg gccttgaggt cattcaggat gacattgacg cagtcctcat gaaaatcccc
     5281 gtggttccgg aaactgaaaa gatagagttt gagagacttg ctctccacca tccgctgatc
     5341 cggcacatac cggatgatga tcgtgccgaa atccggctgg cccgtcaccg gacagagcga
     5401 ggtgaactcg ggacacgtga aggtcaccca gtaatcccgg tcccggtgct ggttggcgaa
     5461 agcctctaat accgaggggt catattggtc ggcgtaaacc gtgccttggc ttcccaaccg
     5521 ggttactccc ggaaccgtct tttttgtgcg gcgcatgctc aactcctctt cagcggattt
     5581 cattatcccg tttcgcatcc tcgatttcaa tggctttttc cggacagacc tgacggcacg
     5641 aaccacactt cgtacagcgg tcctgcaaaa tcaccgcacg ctccagcggg gtgaaggcga
     5701 tcgcctcggc cggacagttc tgcgcgcaga gcgtgcagcc gatgcaccgg tcggacaccg
     5761 tataggcgat cagcgcgcgg caggcgcgcg ccggacagac gccgcgcaca tgcgcctcca
     5821 cttccgcacg aaaatgccgc aacgtcgaca acaccggatt cggcgcggtg cggcccaatc
     5881 cgcacagact ccccgcccgc acgctttcag ccagacgctc caaggccgcc gggtcctccg
     5941 gccgcccctc tccgttgcag agccgttcca gaatctccag catgcgccgc gtgccgatgc
     6001 ggcatccggt acaggtgccg caggactccc gctgggtgaa ggagagaaaa taccgcgcga
     6061 tatccaccat acagtcggtc tcgtcgagca ccaccattcc acccgacccc atcatgcccc
     6121 cggcccgcgt cagggtctcg taatccaccg gcatatccga caagtccgcc gggatgcacc
     6181 ctcccgaggg tccgccgacc tgaaccgcct tgaaccggtg gccctcgccg accccgcctc
     6241 cgatctgctc caccacctgc cgcagcggca gacccatcgg cacctcgacc agcccgccac
     6301 gcgcgacctt gcccgccaac gcgaaagcct tggtcccggg gctccccgcc gtgcccacgg
     6361 cgcggaacgc cgcagcgcca tgacgcacga tccacggaac cagcgctagg gtttcgacat
     6421 tgttgaccag cgtgggccgt ccgaacaacc cgctctcgga cgggtacggc ggccgcacat
     6481 gcggcacgcc gcggcgcccc tcgacactgg acaacagagc ggtctcctcc ccgcacacga
     6541 acgcgcccgc gccttcgacg acccgtaacc ggagcggcac ttccgccccc agaatccctt
     6601 cgcgttccag tagcgccagc gcttcgcgca cacgccgtac ggccagcgga tactcgtgcc
     6661 ggatgtagaa aatcccctcg gaagccccca ccgcgtgagc cgcgatcatc atcccctcga
     6721 tcacgcggaa cggataggac tccaacagca tgcggtccat gaacgcgccc ggatccccct
     6781 catccccgtt acaaaccact accgcatcgg aaccttgcgc ggcgtccgcc accgctcgcc
     6841 atttccgccc cgtcccaaac ccgccgccac cgcgcccgcg caagccggag gcttccagtt
     6901 gcgccaccac ctcctgcggc gtcatctgcc gcgcgcaggt acggtaagcc tcgaacccac
     6961 cgtggcggca atactcgtcc cagtccagcg acgacctttc gcccgcgcct tccgtggcga
     7021 tgcggtgctg acacagccag tacccggcat cactctgatc ccggacgctc agcggaaacc
     7081 ggacgggcgg atcatcctcc tcgcccgcaa aaagctgttc ggtccatgcc cggagtgtgg
     7141 cctgcagccg cgccgcagtc cgggcgggcc ggatgtggct cagcaaaata cgctcgacat
     7201 cccggacgcg gacacgtccg taacgaaaat ggaggccgtc ctccaacagg atatccatca
     7261 gcggcgcgtg gtacgatccg cccgtgcagc cgacctcgcg caccccgacc ggcaaacgcc
     7321 aagtccgcac cacgcggcga atctcgtgca gcacatccgc cgcgccggcc gcggcgcaac
     7381 tggaacaggc gcacaacctg acccgcgcac ctgtctgggg atgcggcacg tcggccgtgt
     7441 cgcggatctg cgtctccccg gcccgcacgt cacgcagcac ggctttcacg cgcgcgggcg
     7501 tcacctgtcc ataaatgaca ccatccacct gtacggccac cgccagcatg cagcacccca
     7561 gacaggccac ccgttcgact gtaaactctc cttccgcatc ggtgtccgac ccctcgggaa
     7621 tacgcaacgc gcgccggaag gcctccactg tgcgctccgc gcctttcaca tgacaggccg
     7681 tgccgacgca cacccggatc cgatgccgtc ccacgggccg caggcggaac tggtcgtaaa
     7741 aggtgaccac tccggtcaac tccgcctcgc tcacccccat cgttaccgct atgcgggcga
     7801 gttcttccgg cggcacatac ccgcaggcgt cctgcaccgc ctgcaatgcg gcgatcagtt
     7861 ctccgggctt ccggtttgcc gacacccgca tccgttcccg cccctcgccc attcctgccc
     7921 cgtcctttcc cgtgtcaatc cgcaccgctg gcctgcccgg gtccctcacg cgtaccacac
     7981 acgccagaga aacaacccgc gcgccggtgc ggacggcacc cgtgccttac gcggtgcacg
     8041 ggacgccaac aggtcgcgta ccgcctcagg cggctccgcc ccctcaccca cccggagcag
     8101 gaatcccacc atgctccgaa cctgcttgta gagaaaacct tctccgcgca cgcggaagac
     8161 gatctcccgt ccccgtttgc caaccgtcac atcgaacacg cggcgcaccg tgctcggcac
     8221 ctcacgctgc ggattggcgg tgaacgaggc gaaatcgtgc tgccccgcga aaaacgcggc
     8281 agcctgccgc atcgcctcca catccaacgg gcgaaagacg tgcgccgcat aaagccgccg
     8341 gtccggcagc agctgcgcgc cgttccacac gaagtagcgg tactccttgc ccaccgccga
     8401 acgccgcgcg tgaaaatccg gccgcacccg cgcaacgctg cgtatccgga tatcctgcgg
     8461 caggcgcgcg ttcagcgcac ggcccaccgc cacgggcgtc atgcggcaca cgagatcgac
     8521 atgcgccacc tgaccccgcg cgtgcacccc ctgatccgtg cggccgcttc catgcacctt
     8581 caccgtgtgc ccgacaatcc ccgcgagcgc ctcctccaag gtctgctgca cgctagggtg
     8641 gtgcggttgc acttgccaac ccgcgtacgc ggtaccgtca tacgcgacag tcagcctgaa
     8701 ccgacgcgcg accggtccgg ggagcgttgc cgccgttggc ccgccaccgg gcttgcgctg
     8761 tccgttcgcc gccatcgctg tcacaccctc tgccgttggg gactacacat acttttcgcc
     8821 gtccggccgc tgagcgatcc ggcgcaacag tttcttcacg tcctctgccc gatcctgggg
     8881 gcagatcagc gtcgcgttgt ccgactgcac cacgaccaga ttgctgaccc cgatcagcgc
     8941 ggtgagccgg ttttccgaga ccacgatgtt gttcagcgat tccatcggct cgcacgagcc
     9001 gaccaccacg ttcccggcca gatcagcaga gaagtgtccc gcgaccgcgg gccaggaacc
     9061 cacgtcatcc cagccgaact ctcctcgggc caccaggatg ttgggtgcgt gttccatcac
     9121 cgcgtaatcg atcgagatgg cgcgcagcga cgggtagacc ctttgcagca gattcgcgag
     9181 ttccggaccc ggggcggccg caaccaccgc atcgcagagt tccgccagat cgggggcgaa
     9241 ccgctcgagc gcctcgcgca tgacgtcgca tttccagatg aacatgcccg cgttccagaa
     9301 ataggttccg ctttcaacgt accgcgcggc atggtacgca tcgggcttct ccacgaaccg
     9361 cttggcccgg ttgaagaggg tcacggtccg ggtttcgacc gcctcgccca cctcaatgta
     9421 gccgaaccct gtggcgggcg tgtcgggctt gatgccgatc gtgacgatcg cctcctcaca
     9481 acccgccacg cggatcgcgt ccgccagcgt cacgcggaag gtctccacat cgctcatcaa
     9541 ctggtccgcg gtcaggatac agaccacacc ctcgggatcg cgctgccgca ccaggccgca
     9601 ggccaccgcg acggctggcg cggtatcgcg tttacacggc tcgccgatga tgttggcgtg
     9661 cggcacatgc caggcggcct ccacagtttg ttcgacgaga tgttcggccg taatgatgag
     9721 tatccggtcc gccgggatca gcccgctcaa ccgttccacc gccaacgcca gcaacgggcg
     9781 tccgccgaaa agggaaatga actgtttggg ccgggccttg gtgctcagcg gccaaaaacg
     9841 ctcgccgctc cctccggcca gaatcaccgc atacgcgtgt cgcattggga aatcctttct
     9901 cactcgattt ttgtttcaat caacttccat agcatactga aactgccgca gagcggaaag
     9961 cgatcttttc atgggacaag tccaattgac tttccccctc ccccctgcat aataggtgtc
    10021 aatcatggga accggtttgt ttcgggttcc cccgatgaaa gggtagcgga acaggagaaa
    10081 caatgaatcg caagagagat gcgctgatat cgatgaccgt tgcaatggga gtggcggcgt
    10141 gttgcttcgc ggagtttgaa agcggttgcc cggagtatgc gccggaactg aaggaccgct
    10201 gctggatgtg ggggcataag accgggcagt atgatcagcc gaacggttgg tttcgcattc
    10261 cggtttcccc ggacgtgacg atggcggagg cctgccggta catgggcata ccgaacgtct
    10321 ccattgtcat ctggcacatt ccagacgaga agttcatgtc cgagtttacg gacatgaagc
    10381 gcgtcgcctg ggtcgtggac aacggcgacc ttcaccttca cggcagggac atcctgcccg
    10441 agtttctggc gttcggcgaa aagaaaatga aaacgatgcc caatctcatg gggttcgagt
    10501 tcgacgactt cttcgaccag ccgcgcaagc cggttcgaaa ggagaaactg gcggacggca
    10561 cggaagtgaa cgtgttgccg ggatcgcgta cgctggaaga gttgcgtgcg ttgcgcaagc
    10621 ggctccattc ctatgaccgt ccgatcgaca tgcgacttgt cttctacgaa cgggaccttg
    10681 accatcccga agagacgaag gcggtcgtgg acatcttcga cacggtgatg ttctggacgt
    10741 ggttcggcaa gaatgtccag gctctcgaga acaacttcaa agcctatcgc aagatcgcgc
    10801 cgaagaaacc gacgctgctg ggcatctaca tgtgggattt cggcggctcc cagccgctcg
    10861 acgcgacgtc gatgaagctt cagttggact ttgccctcaa gctcttccgc gccggcgaag
    10921 tggaggggtt catcttccat tgcacgcctc tcgtcaacaa aaacatcgag gccgtagaac
    10981 ttgcgcgccg ctggatcatc gagcatggca acgagaagca cgggggcgga gaacagcgcg
    11041 ttccgccgat tccggaaccg ccggccttgc gtccggaaca gacgatgacc gatttcggcc
    11101 gtctggcatt tgaggaaggg aagaagccga acggcgcact cctgctgacc ggacccggca
    11161 agatccggat tgtcgagcgg acggggcgga tcgcctggga aaatgtcgca caggcgaatc
    11221 ccgagaaggc gttttatcat ggcgggcacg tttatttcga ggaggacggc attcagaagc
    11281 gcatccgcta ttcgctgccc tgcgatcggc tggcgaaggc ggaagtcgtg ccggaggcgc
    11341 agcgggcaca ggtgccaaag aacaagaagt tcgacagcgc cttgctcgat tgcctgaaag
    11401 acaggtccgg atattctggg gcaatgaacc tgcaacgttg aacgagcacg accttcaggc
    11461 caacgaaggc atgcggcacg cgctggcgga caggctcttt ccggtttctg gtttcgatcc
    11521 agatccggat cgtgatttcg atagcgtttc gatatcgatg tgccgaccga tcactcttcc
    11581 cgcttgaccc gcagggcgga ttcgttatga tacgcgacat cacacggttc cagaaagagg
    11641 attaaggagt aacgtttcat catggcgaat atcaaaagtg cgattaaacg tgcgaaacag
    11701 tccgaagcgc gtaacgtgcg gaatcgggcc gtaaaaagcc gcgtgctgac cgcgcgtaaa
    11761 aaagtgttgg cggcgatcag tgcgggcgag aaggaggcgg ctcagaagct gtattcggat
    11821 tacacgtcag ttctcgacaa ggcagccaaa aagggcgtga tcccgaaaaa caccgccagc
    11881 cgcaaaaaat cccgcgtggc gctgagcatg cagaagatgg cctgaaggcc tgaggttctc
    11941 tttgggtcaa gaccggtcac cgcgcacggg cggggtgacc ggcttttgtg tttcaatccg
    12001 gaggcggatg acatgcgtga gctggaggtc agcgatctcg gacgcgatgc cggatacaac
    12061 gaggtgctcg ccctgcagga gcggctgcac acgcgccgcg ccgcgcggga gattccggat
    12121 accctgctgc tgctggaaca tcggccggtt tacacactgg ggcgtagtgc cgcggcagcg
    12181 catgtgctct attccgagga acgcctgaaa gaggcgggta tcgaacgcgt gcagacctca
    12241 cgcggcggcg atgtgacata tcacggaccg ggccaactgg tgggctatcc gattcttcat
    12301 ctgggggatg ccggactccg tgtgctggag tatgtgaccg ctctggaaga aacgctcatt
    12361 cgcgctgtcg ccgatttcgg catcgtcgcg gcacgggatc cgcggaaccg gggcgtttgg
    12421 gtcggtaacg cgaaactcgc ggcgcttggc atccgcgtcg cgcatcaggt caccatgcac
    12481 gggttcgcgc tgaatgtggc gccccggctc gatgattacc gcgggatcgt ggcttgcggc
    12541 ctgacggatg ccagcgtcac ctcgctgcaa cacctgctgg gggaagcgcc cgacatgccc
    12601 gccgtcaaac agagtgtggt ccgggcattc cgagaggtct tcggatacga ccgcaacatc
    12661 acggcgtgat ccaacgcgcc gcagcgcgaa atggcctcag ccgcgttggc gtttgcctgc
    12721 cgcaccgtcc ttttccggcg gctcctgcgg ctcgaacggc agtcggaacg cctcgtcgaa
    12781 atcataaacc tgattaaaat cctctaagct gtcactctcg gtcttcccca accggcccgc
    12841 ctcgaccaga ttgaacacga tcgcgccgaa atccgcagtt tcgtgtatcc cccactcggc
    12901 gaacacggtg tacgccagag ggccgtactc gtcaagcgcg aacgcccgga ttccctccaa
    12961 cagttcccgt ccgctcacat cccggccgtg ttcgggctct ttttcccgga tggccttgac
    13021 cgtactgtcc agagcctcca tcaaaaagag gtacgcgtcc tcgtcataac gcggatcctt
    13081 ggcgcagatc ccctgcacaa cctctcggaa agagatatcc cgacccttca tacgccagcc
    13141 gccacagtca gtttccggta cacggactcc atctcttccg cagacgcgta agcgcccgaa
    13201 atccaattga tcgcgcgtcc gttccaacgg ggcaccacat ggaaatgcac atggggcacc
    13261 acctgcgtcg cgcaacgtcc gttattctgc aggatgttga acccgtcgca cggcagacgc
    13321 tccaacagca acgacgccac gcgcttgacg agcggcatca ggtgcgacag gacggagtcc
    13381 ggcaattccg tgaacagggc cgcatgcatt ttgggaacca ccagcgcatg ccccggctcg
    13441 aacggcgcga tatcgagaaa cgcgatcgct tcctcattct cgaacaacac tttcgcagga
    13501 atctgccccg caatgatccg gcaaaacaca cactcaggat ccgtcatgac ctctcctttt
    13561 tctttccgcg ccgaaaacgg ccgtattaaa acaaagactc cccagacaag aaaggatttt
    13621 attggcgcgt ttcccaaacc gttccgcgca tgggtctcct ccccgaatca ttccggcagc
    13681 ggcggcaacc ggaacacatg cgattcctcc tcaaccgcgc acaggatctc gggcgcgggg
    13741 aaaccgtaac cctcgcacaa caggcggatc acctcatcga gaaacgcctc gggagtcgag
    13801 gcaccgctag tcagaccgac cgcaggctcg tctgccaagc ccgccacctc cgccagacac
    13861 gacggcgtgc tgacaaggca tgcccgcgcc ccggcggtcc gggcggtctc gaccaacctc
    13921 tggctgttcg aactgttctc cgatcccagc accagcacca gaccgcaacg ttccgccagc
    13981 aggctcaccg cccgctgccg gttgcgcgtg gcatagcaga catccgtgct ggcgggcacg
    14041 atcagacgcg cgccgaagcg tcgccgcaac acccgcatca ccccctccac acgagccgca
    14101 tccagcgtgg tctgagtcac caccgcgacc ggttgggttg acggcacatc cagcgactcc
    14161 gcctccgcct cgcactccac gatacggact tggtccggtg cctccccagc cacccccacc
    14221 acctcctcgt gatgtcggtg accaatgcag accacgcagg caccctgcgc cgcgaaccgc
    14281 accacctcgt gatgcacctt catgacgaac ggacaggtgg catcgatcac ccgcgcaccc
    14341 cgggccgccg cctgccgccg cacctcgggc gaaaccccgt gcgccgaaaa gagaagccgc
    14401 gctccctgcg gcacttcggc tggatcggaa acgaacacca tgcccgcatc ggtcagacgc
    14461 gacaccacct gccggttgtg cacgatctcg ttcagacaat acacccgttc gccgggacag
    14521 gctgccagca ccgcctcggc cgtggccacc gcccgggcca cccccgaaca gaaaccgtgc
    14581 ggcgtcacca ccgtcaattt gcgtctgtta cacatcgtca tgccctgtct tagccgccgc
    14641 gtcgcgctcg agttccgtca aaatccgctc ggccggggtc agcacatttt tttcgcggtt
    14701 gcaggcgacg caggcaggca ccacattccc ccgggtgctc gtacccccgc gcgccacggg
    14761 caccacatga tccaaggtca gggcgtccgc gccaaccgtc ttgccgcaat accggcacac
    14821 tcccggccgg atctgctcac gccaccatgc actgttgcgc agcacgcggg ccttcgcgcg
    14881 ttcacgcgcc acatgccgtt cgtccttgcg gatatcgacc cactcatcca taccgtcccc
    14941 ctaccgtcac tccgtcgtct tcgtctccgc attccgcatc cgctccagaa ggcgggtcgc
    15001 gtgatttgcc tcggccgcgg ccagcgcccg gaacgtttcg cgcaggcgcg gcaacgtgct
    15061 cttctccgcg aggatctgat aaaagcggga catggcgcgt tcgtgctcga tcgcatgctc
    15121 cagttcgcgg ggagccttca cggcatcgtg catcatctcg ttgaacggcg gcagtgcgcc
    15181 agccatctcc gccgtgatcg gaatgtcccg gcctgccccc gcctccatcg cgccgagcat
    15241 ggcctgatgg tgccaacgct cctcctgcgc caagcgttga tagagcgctt tttcctccgg
    15301 gtcaagcgaa cgggccgcca acgcctcata atcctcaaac gccgccttct caaacactgc
    15361 ggcctgctgg atcacgaaac ggccgatcca gtcggagaag gcggaaaccg gcggcgccgc
    15421 cgtccgcacc ggcacggttt tcgtcccgaa cacctcgcgc agcatcagaa caccgaacaa
    15481 cagaaacagc aggccgcccg cgcatttaat ataacgctcg tccggcacaa aacgccggat
    15541 caggcctccg gccaagaccc cgatggcggt ggaggcgacc agcgccgcca ccccggccgc
    15601 gaacaccgtc cattttgagg acgaggccgc ggattggctc atcaccgcca actgggtctt
    15661 gtcccccagt tcggcgagaa agatcaaaat gaatgtgctg aaaaataacg gtccgttcac
    15721 tccctgctcc ttgttggatg aaaaacagta tacacggtca ccccgcgcat ccgccacctt
    15781 caacttgcca gacctcgcac atggcagatg aggccaataa aaaacaggtt gtttctcaca
    15841 tcaagacacg aaggagaggg gtttgcctac gtgggagaga tgacgagact caggaaccgg
    15901 ccatcctcct caaagagcaa ctgcctctcg ttcagaaatc caatctctct ccgcacggca
    15961 ttctcttcga cctctgccgc gacagcggcc tcgctgatcg tccggggcgt ctcccccatc
    16021 tcttgcagca gccgctcgga aagatcggaa atccggtaac gcccggttcc gcccgagcgc
    16081 gaatcgtgaa tgcaggcact ttgcgcgtcg cgatagagca gcagaaacgg ccgttcctcc
    16141 ggaccgcacg cccaccgctc ctgccaggtc tcgatcaacc cgcccagccg gtccaaccat
    16201 tgttcggccc atactggatc ggcgttccgg tccgtaaaac ggtaacacaa tccaaacagc
    16261 gtttccggag cgaagggata gatgtagcgc aggctttcgt gcggctccaa atccaggccg
    16321 taggccccgg cctcttcgaa atagcgggaa tcgcgcacga actcgatcgg aaagactccc
    16381 tccggcggct gcagatgaac caggcgcggg atatcgcgct cgtacttgcg gtaaacctcc
    16441 tcccgctcgc ccggcgagaa gagcaggaga ttccactcca cgtacagcgc gtgacggagg
    16501 cagtctttca ggaaccggag attgtcgaac gccgtgaccc ccttgcgcat gagcgtcaga
    16561 ctctctgtgg aaagcgcctc cacgcccggc tggacacagc ggatgcccgc ccgacacaac
    16621 acacggatct cctccgccgt gatcgcggac ctcgtctcgt atttgatgaa gacgccctgc
    16681 ggcggtgcca gatgcgggaa cacctcgcgg aaataattcg gcggcaccac gttgtcgcag
    16741 gccacgaacc gcgagacccg ccccgcatag ccgagaaccg accggatgtg cctcagggcc
    16801 gcttccggtg gcagggcctc gaaccgttcc tgaagatcgt tcaagccgca gaacgtgcag
    16861 cgccgccggt cggcccacca acaccctcgg gaggtctgca tcagcagaaa cggctggcgt
    16921 ccggaatccc ccaacacggc atcaaaatga tccagaaacg agcggtagtc gagcggcaca
    16981 tctttccgga tgtcgagttg cgcaccgaca ttgcacgccg gcggcagcgc atccgccaca
    17041 ccccgcgcga aaacgcccgg aatcgccgga cgcgtgatcg gaagacccgc gagacgacgg
    17101 ccgaccaact caggaaaact gaccagcccc ggtccggaaa acacggcatc cacacagggc
    17161 gcatgctcga ccagcgtctt tcccggaacg cctttgaccg caggcccgcc gaacacaacg
    17221 gtaacctccg ggttgcgcag cttcagccgg ctggccatcg ccagcgaggc gaccgtctgg
    17281 aagaagcaga gggaaaagcc caccagatca gctgaatcca gcgcataccg gtcgatcaac
    17341 tcgtccagaa aagcgaaaag ttccccgcgc cgtgcgcgcg cgaactccgc gacctccgtt
    17401 gcctccgtcg cgccatcgta gtaataccga ccgagatacg cctcgatgtt atcctccgca
    17461 tcgggaaacg ccgcagcacg gaagaaccag tcggccagcc cggtcatccg gccgtagggc
    17521 gaggtcgccg ccccgtagaa tcctgccgcg tccggcaacg ccgcgacatc cagattcaga
    17581 taatggatcc ggacatcgac ctgcccgccg aaccgctgtt tcagcaccga cgcgatctgc
    17641 gtgagaccga tcgccggacc ctccggcggc gcaaacggca tgtagatcag cgcgatcctg
    17701 aggcgtttgc cattttcccc gttcattccg gggttcagga tgccacgttc cggacaaacc
    17761 gtcgagccca agaatttgcc cggcggtcac gccctgcatg cgcgacgaag acccgcgcca
    17821 gccgcgcgga tcagccgctc cgccgtatcc tcacgcggcg cgccacccac gagcggcagg
    17881 gtcccgtacc cgccgcggcc gaagcacgcg gccagcggca ccccgcggcg aggcgtgatg
    17941 agtccattcg caaacccgac gcccatcgct ccctctttcc gtccggatca ccccttcacc
    18001 gccaccgtgc gtccgccgcg ttgcgcggat gcgtgggccg cctcgcagat cagcacattg
    18061 tgcagccccc cctccccgct cagaggccgg ccactcagaa tcgaggccgc atgctcctca
    18121 atcacccccc ggtagatgtt gaccggtttc gcgacccgga ggcgccgttg ccgatcaaag
    18181 gcatccaact ccagccgcat cgcgaacggt tcatccggat gcccggaaag ctggaacagc
    18241 gtgccatagg cgcgcaacac cgcctgatcg ccgtaaatct catacccgag atttgaaagc
    18301 gtcccgccca aaccaccgcg cacgtcacaa aacgagacca gcacggaaca ggtgatcccg
    18361 ttctccaaat cgcactggat cagcgcaccg tcctccaccc ggatggccat gcgcttgggc
    18421 atgtacaccg cacggaccgc ccgaatccgg ctgtccaaaa tgaactcggc catataaaaa
    18481 cagtgactcg ccacatcccc gaccgggcca cccatctccg cccggttgtt acaacgccaa
    18541 gtagccgcct ccgccggatc gaatccgtac gcgaactgca tgtggaaaca ggcgtcgctc
    18601 accttgccca gagtgccttg ggccacctgt gcgcgtgcct cccggttgaa tgtgttgtgg
    18661 gtcatcatgt ggtcgaccga gaggctgagc tttttgcggg ccgccagctt gaccagcatc
    18721 cgggcatcgc gcgcgttcgg cgccaacggc ttttcgacaa tcaccggacg gcccgcttcc
    18781 aacgcccacc gcgccagcgg cgcatgcgtc agattgttcg tcgccacaaa aaccgcgtcc
    18841 acccccggat ccgccaaaac ggcatccaga tcgcgataca ccttcaatcc cagggcttgt
    18901 gcagccttac gccgtttcgg gttcacatcc gtggccccga ccaacaccgc ctgcttcaat
    18961 ggccggaacc gggacacgtc acacgcaaac ccctctttcg cgatccggtt ctccgcaatc
    19021 ccgccgaacc ccaccaacgc ataccgcact tgattcatcg cgtacccctc atcctaaccg
    19081 taaccggccc atctgtttcc aaaccccgta cgccgcaacc gcggcgatgc ccgacatgac
    19141 acaccccagt gcgatccggt catacagcag ataaccaacg ccgaaaagcg cggcccacac
    19201 cagcaggcag cccagcagca tacacaccag accggccggc acattccaac acggttccgt
    19261 tcccgcaggc tcatcccccg ccgcttgaat ccgctctgtg atccgcttcc accccggacc
    19321 gccgggacgg attttccggc agaaggccat cagcgtccgg tcgtcaaccg gcggggtcag
    19381 ataggtcacc gcgacccata ccaccgtggt gaacagaatt cccgacacca tcttccatga
    19441 ggtgaaatcc atcaagcgga accatccggc atcggccatt gcggattcga tgcctaacgg
    19501 cgcggccccg aactggaaaa acagggccac cacaaaagag accgtcatgg cggccacctc
    19561 gctccacacg ttgatccgcc accagaacca gcgcaggatg tagagcaggc ccgtgcccgc
    19621 gcccacctgc aacagtacgt ggaagatgtc tttgaccgtg gtcatgtgaa acgcgatcca
    19681 ggctgaaacc aacagcatcg ccagcatggt cagccggccg atgcgtaccg catggcgcgg
    19741 cggggcctgc gagcggatga agcgcaggta aacatcctgc accacatacg acgagcccca
    19801 attcaacagc gtggccacgg tagacatgta ggctgcgatc agcgacgcca caatcagccc
    19861 ccgccaaccg ccgggcatgc gcgagaccat acccggatag gcgatgtcgt gaccgagata
    19921 ggtgtcgtcc acgcgcggga aagcacgcgc cagcgacccc acgccagccg catccgcgcg
    19981 cttcaggcgg accgcctcgc gcacctcgtc cggcagcgcg gccttgtcgg cagcgtatcc
    20041 gcgcacctca gccgcatgcg cctccagcca catccgggca gcctcccgtt ccggttgcgg
    20101 ggtcagaggg aaaaccacca tcgaagcgag cgccacgaga atccagggcc agggccgaac
    20161 cgcgtagtgc aacacattga agagcagcgt gccgccgacc gcgtgccgct cgtttttcgc
    20221 ggaaagcatc cgctgcacca cgaaaccgcc tccgcccggc tcggcccccg gataccacac
    20281 attccaccac tgcaccgcca gcggcagaat catcacggtc atcagggtcg agagatcatg
    20341 cgacgacggc cggaacaacg cgagattggg aaccaccgca ggatgcgtga acagttctcc
    20401 gagggacgtg acgccgtccg ccatgccgga acggaccgca tacatcgccg cgaacacggc
    20461 accggtcatc gccacgccga actgataaaa atccgcccac atcgaacccg tcagcccacc
    20521 caaggtcgca tagatcgcaa ccccggtcat ggaaaccagc agcgacatat gcggcgacaa
    20581 cccgaacaga atctgtccta ttttgatggc cgccagcgac acgccgccca aaatgatgag
    20641 gttatagaag acgcccagat agatcgcgcg gaacccgcgc agaaacgcgg ccgaccggcc
    20701 actgtaccgc aattcgtaaa aacccagatc cgtgtccagc cccgagcgtc gccacaaacc
    20761 ggcgtacacg aaaaccgtca gcatgccggt cagcagaaac gcccaccaca cccaattgcc
    20821 cgccacgccg ttcaaccgca cgatttccgt gacgaggttc ggcgtgtcgc acgaaaaggt
    20881 gcaggccacc atcgagaccc ctaacagcca ccaaggcatg ttccgccccg aaagaaaaaa
    20941 cgattcggtg ttctggccag agtaccgggc ggcccaagaa ccgaccagca cgaccgacaa
    21001 cagaaacccc acgatgatcc acgcgtccag ccaagtgatg ggtatcatac ggcactccaa
    21061 aggagaaacg ggaaaacggg gctgtggcgt cgatgccaca tttcccgtcc cacacttccc
    21121 gtttcccgtc tcccgatccc tatttcatgt atgccttcgc cactttcttg aaagcatcga
    21181 tacaggcctg catgtgctct tcggtataaa ccggatgggt gaagaaacac atcgtgcggt
    21241 cggtcatcca ccgcgcgcgc gggcacgaca cccggctgta gtcgatgtta cgcgctttgg
    21301 gatcgaagaa cggatagttg gcgacgccga atccgttccg ttcgaggtag gcccgttcct
    21361 tgtacatctc cggccagagc acgctgtaga cgggcacccc ctccgccgcg atcgccttga
    21421 tgaactcctt gatcgggcag gtcagtttcg acgtgtcgag cacaaacggt gcccaccagt
    21481 acgcattccg gcgctgcggg gtgtctactg gcgcatggag cacgagcgga tgcttctcca
    21541 acgctttttt caacatcctg ccgttgcgga tccgattctt gagattccaa gaatcaaaac
    21601 gcttcagctc gcacagtccg atctgcgact ggatctccgt catgcggtag ttaaagccga
    21661 cccgcttgtg gatatacatc agcttgcctt ccatctccaa caggttcagg cgttccttca
    21721 cgtcatagcc gtgatcgcgg aacgaacggc actcccaggc gaggtcgtcg tcattcgtga
    21781 ccaccatccc gccctcgccg ccagtggtga agtgcttgct ctggcagaaa ctgaaacagc
    21841 cgacatcccc gagggtgccc gccttctccc ccttgtacag gccgccgaaa cattgggcgc
    21901 agtcctcgat caccttcagg ttgtgtttct tggcgatctt caggatcggc cccatgtccg
    21961 ccaccacgcc gtagagatgc accaccacga tgcctttggt ccgctcggtg atcttctcct
    22021 cgatctgccg cggatcaatc tggtggtcga accccacatc cgtgaaaacc ggcagcgcac
    22081 ccgcctgcag gacacagaac gacgacgcga tgaacgagta agacggacag atcacctcgt
    22141 ccccgggccc gatacccaac gcggcgatcg cggtgtgcaa tgccgcggtg ccgttggtca
    22201 cgctgatggc attgcgcact cccagccatt tggcgaacgc ggcctcgaac ttcatgccga
    22261 ccggcccggt ccagtagttc accttgccgc tcaccagcgg ttccagcgcc gtcttcaggg
    22321 tttcttccga aaaagagggc cacatcggga accccttatc ccatactttc gcgccgccct
    22381 taagggccag cgccgcctgt tttgaaccac tccgtttcgc tgctttcctc atggtcgcca
    22441 tgtgcttctc ccttcggttc gggttccgct gtcctcccct gcccgatttg cccgcctctc
    22501 tcacaccgtc gtgatcttgc gttggacagg atcgtaaacc gctttgcgcc cggtttcata
    22561 ggatttcatc gccatgatga ccgccacggc gtgctggtag ccggcctcca gcgaagcgtt
    22621 gggcgttccg ccactgcgca tgcattgcag ccagtcgaga taatggtcgg ggtgctccgt
    22681 aagaggaacc tcgttcttcc cgcggatgga gccgtcacgc aacggcgccc cttcgcagtc
    22741 gtagaccggc ttgctccaat tcgtgaaatc gagcgcgccc ttgtcgccat acagtttccg
    22801 gacgttgttc gcgccgttgg caaggttgtt ggacgagctg accacgaacc cttccggata
    22861 gagccaagtt gcctgaatca catttggatt ggtgaattcg tgagcgtcca gccaggtctc
    22921 gttgatcctc ccgagacaga cgcacgaggt ggggaaggtc gccccggtaa tgaaatgcac
    22981 gagatcgata aagtgcgagc cgagattcgg aatcggaccg cgcgagaact cgtaatagcc
    23041 gtaccagccg gagaagagat cggcgcggaa cggccgcatc ggcaacccgt gcagaaactc
    23101 tttccagtcc agatccgcct cccggacatc ctctttgaga taatgatacc aatacggacg
    23161 cccggcgttg cgacactctt ccacacgcga gatcctgccg aagatcccgg tccggaaaag
    23221 atcgcgcgcc cccaccatcg agggaagact gcggatctgc gtgccgatct ggatcaccgt
    23281 gcctgcggcc cgggccgcgt cagtcgcacg gaccagatca tcgaactcca ccgccagcgg
    23341 tttctcgcag taaatatgtt tgcccgcccg tgccgccgcc tccagatgcg cggtgtgatg
    23401 cagatcgcag gaggcgatgg tgaccgcgtc cagatcccgg cattcgagca gctgacgata
    23461 ggagctgaac tgttgcgcgt cacgtccgaa ccactcccga accagaccgt tggccgcttc
    23521 gcgggccacg cgccaaggat ctgccagcgc cacgatctcg aagggctgcc ccttcacatg
    23581 attgtaaagc cccttcatgt gcgcgttgcg tccgcgcgaa ccgcagccga tgatcccgat
    23641 gcggatgcgg tcgttcgcgc cgatcgaacg gcggtacgtg gcagccgcca ccgatgcgga
    23701 agccgatgcg acagccgcgc ctcctgtgag tttcatgagt gcgcgacggg tcaatgtacg
    23761 gttcataaac gttcccctcc cggtttcagc tcacgttccg gatgccgacc ggaaacgtcg
    23821 cggagccaac ccccgcgtgc cataccgaaa ttcctttatt gtcgcccagt atacggcaac
    23881 ccttccggcc cgacaagttc cgtttccggt gctgtaaacc tttttccaga atatacgcga
    23941 aaatccggac ggaacgggaa ttcattcgcg cggcaggccg ggatcgtaac gtttcccgcc
    24001 gttccgcaac gcttcgtaca cgcgccgctc ttcagccaac cgcgcctgat atttcttttc
    24061 gcgttcctga tcccggacgg acgccacaaa cccctccatg tccgcgcgcg gccgttcgtg
    24121 cagccgccgc gcacccgcgc gaatcagcgc cagcgcctcg gcacgttccg ccgaattcgt
    24181 cgccagcccc gacagaatcc ggctctcttc cggccgggcg aaactgacct gcgaccgctg
    24241 ccgctggcca tgcgagatgg aaatcgccac gccggtgagg tccttcagcc gcttcaactc
    24301 gtcttgactc aacggactcc gtccgccggg atgatcggga tacgcgctct cataatacgg
    24361 atagtacggc gcattgaggt ccacccatgt gatcagccgg tccaactcct cgggtgataa
    24421 cttgaccccc gcgtggccgg cacggacctt ccggatcaac ttggaccgat ggctacccca
    24481 actgtatgcc tgctggatct cggccggtcc gccgcccacg caggtcaagg caccctgaac
    24541 ccacaagtcc gtgtacgagg tgctgaagat cacatcgcga tcccccgcca gattaagctt
    24601 cttgcccgcc tcgtttccgt agtcgtgaca ggagacgcac cgcctgttga aaatcggctg
    24661 cacctcgcgt tggaaacaga agagccgcgc cgcgccgcgc cagccggtca ggcgatccgg
    24721 tttgcgccgc aacgccttgg gccgtgtcgc aggcggcggg atgtcgccca cgcgattctc
    24781 atgacaaccc acgcacccgt aggtctcgcc cggctgcaca taggttccgg aacgcatgga
    24841 ctgcaccatc atgccgttgg cgtccaacac ttggaaaaac acaaacgtgt tgcccggaac
    24901 ctcgaaatag gcgctgccgt cctcctccac cggaaccgtg ccgagaatcc gcttgttttc
    24961 gaaattatgc cagttcatgc ccggtgcctg ctcaccttga ccgaaccatc cgcgtaccga
    25021 aaagttgcgt ttctccggtg attccaccac gcgcagatag cgaatcgtgc ccggtgccac
    25081 ccctgccata tgcgtaccga tcatcgcatt ctgcacgtaa aaaaaaccgg gcgcgttcgg
    25141 gtcgtcgaaa ttccggcggc gcgggtgaac cggcggtgcg ggccgcggtg ccagcggcat
    25201 cgggtcgaaa caccccggcg cctcgacatg gaccaacacc tcgttaccgt gcgaatccag
    25261 atagtacagc cccgtctcct cccctttgcc cgttttgcgc gcgcacagga aatggcggtc
    25321 atctaacggg tatggatcct cgtatttgat ttggagccct accgtcgaat caaaattctg
    25381 ccctgcggtc cggatgcggt ttgtgaaagc gaccggccag gtgcgcaata cgggttccaa
    25441 cccatccacg cccttcgacc ggtcgatcag acccaacgcc ccccagggac ggtcatggca
    25501 ggagcccagc acggcaatcg ccaaatgact ctgcgagagc gcccgtgcat cgatcacggc
    25561 acccgggctg gtggtgttgt tgccccagta gagcgcatgg cccgtgccgt ccggattgca
    25621 cacccataaa ccctgcgcgt caccgaagtt gcggtccaca tactcccaac ggtcatacag
    25681 cacacgtcca tccggcaaca gcgaggcgtg cccctcaaag agcgtggatt tcccgatctg
    25741 gtggatgttc gcgccgtccg cgcccatacg gtagagattg cacataatgt ggcggttgca
    25801 catgcagtac ttgggctcgc gggaactgga gaagagaata tccccgtccg gcagccagat
    25861 cgggtcgatg tccgatacgc ccggcgccga ggtcaactgc cgcagtccct tgccgtcgct
    25921 gttgatcgcg tagatatggt aatcgtcccc cttcccgcgc cgcatcgaaa agaggatttg
    25981 gccaccgtcg aagctcacgt ccggatcgcg caccgtcaca cccggccccg gtgcgaaaag
    26041 cgtccgaacc gtgccggagg cggcgtccaa caccttgagc gcgccctcgg tgtcgtattt
    26101 gtccgtgttg atctccccgg tctggaacat cgtctccgtg ttgtggtggt ccggtcgata
    26161 ttgtttgcgc gtcacgaaga ggatcggctg cgcgttcatc agcgggttgc gcgccaccag
    26221 cgcttcccgc gccacagcgt caagttgggc ctcatctccc gcggcctccg ccgcacccaa
    26281 ccgttccagc aagacagcgg aggggtaacg cgcgccgaaa cgctccgcca attcgttgat
    26341 cgccacgcgc aaccggtccg cggcggctcc gcccctggcc cgcgcgatct cacgcctgcg
    26401 ttcccggaac cgttgctccg tggccgcctc gtcgatgatc ccctggcaga ccagactgtc
    26461 cagtgtgatg tgcccccagc cgacttccga ctggtccgac acccggaaaa aaaccggctt
    26521 gcccacctgc tccggcactg tccagacacg ttcgagtatc cgctggctgt ttccgccgcg
    26581 cgcatgcgcc aactcccgcc cgtccaagga gcacaacgcc acgtacacgt ccgcgccttt
    26641 tccgcccccg acccgcaacg aaatccgcgg gccacgcaac accaccaccg gtgactccaa
    26701 aatgcccgta aacacgtcac gtgggctctg tccgtccgcg ctttccagag tcgagaggaa
    26761 aaaacggcct tccttggtgt acggtccgcc tccgtgatgc tcgcggtccc gatcacttac
    26821 caccttcccg aacccgccgt ccagaatacg ccagccttgc agatccccgc tctcgaaatc
    26881 gaaccgtact tccgcagcgc cgcaccccaa tacgacgcca atcaaaagca tccccctcaa
    26941 ccgcccattc atcactgttc ccttccctcc ggtaaggacc gccttgcccg atgccgaaca
    27001 ataggcgcga attccgtgtc cggcattcac cgaacaggaa aattttgtca caggactcaa
    27061 cccggcgcaa ggccaatctt ccgtaagcgg gataaccgga acgctgaaca gggacgccgg
    27121 ggcgggcacg gttttcatgc gacaaaacag catccccttg ccaccagtcg attacctccg
    27181 tattgtctcg cgcctttttt tttactacac tccggaccgt tgcctgtgat tccgcgtatt
    27241 ctgcggcggg cttgttccac tatgaatcgt ttcgcctatc tggtccggcg cctgctgttg
    27301 gtgattccca cctttctggg gatcacgatg gtgtgttttg cgctgacgcg ctttttgccc
    27361 ggcggtccgg tcgaaatcca gctgatgaag atgcggaatc tcggcggggc gaatgatccg
    27421 ggcagtgccg cggtcgggca ggtcaccgaa gagtaccgcc aggaattgac ccgccagttc
    27481 ggattcgacc gccccctgcc ggtgcaatat tacgactggc tcgtgcgcaa acggatggga
    27541 ttgctcatcg cctcctacga ttaccccaac cggaccgcct ggcaactgat cgcctcccgg
    27601 atcccggtct cgctctggtt cggcatcacc ggattcgtgc tgagctacct gatctgcatc
    27661 ccgctcgggg tggccaaagc cctgcggcac cgccagccct tcgacgccat ctcgagcctg
    27721 atcgtgttcg cgggatatgc catccccgcc ttcgccctcg gcatggtgct caaaatgttc
    27781 ttctgcggca cggtcgaggg gctggccgac ctcttcccgc tcggcgggtt cgaatccgac
    27841 ctgccggcca ctgcgggttg gggtgcccga atggcggacc gcgcctggca catggcgttg
    27901 ccggtgacct gctatgtagc gggaaatttc gcggtgctca cgctgatcat gaaaaacgcg
    27961 ctgctcgatc agatcgccag cgattacgtg cgcaccgtgc tcgcgaaagg cgcgacccgc
    28021 cgccgcgcta tctgggggca cgcgttccgc aacgcgctca ttccggtcgc gaccggtttc
    28081 gggtcgatcc tcggggtgct gttcgcgggt tcgatcatca tcgaaaacat cttcgagatt
    28141 cccggcatgg gccgactgag ctgggaggcg ctcaccggcc gtgactatgc ggtgtttctg
    28201 gcgctgctcg cgttgacctc cctgctgcaa ctcgcgggga acctgctttc cgacttctgc
    28261 tacctgttga tcgatccgag aatccacttt ggtgacacat gaggcgattt ccccgctacc
    28321 ggctctcgcc gctgacacgt aaacggctgg agcgcttttg gcgcatccgc cgcgcacgcg
    28381 tggcgctcat attgctgacc gccctgttcg cggtgagtct gtgcgcggaa tggctctgca
    28441 acgggcgccc cctctttctg cgcgtgaacg gccgcacctg gctgccgttc ctgcaacgcc
    28501 tgacactgcg cgatctggaa ggccctgatg cggaggcgac ccgcgtgaac ttccgcgcgt
    28561 tcgttcgatc acccgccttc accaccaacc cgctcaaccg cgtggtctgg gcgccggtgc
    28621 cgtacagccc cggtgacgtg gtcgacgccg ggacgctcag ccgtttccgc actgtccatg
    28681 tttcggtcgt gcccgagatc cgcgtgggtc gcctcaacct gctgccggac ggcacggtga
    28741 tccgtgcgga aggctgcgaa cccttcttcc ccggcgaggc gcgtgtaaac ggcgtggtgc
    28801 tcgaccggca ctggcatgtg ccggaaaccc tgcgcgcgtc gctggacgca cgggccgcgg
    28861 gccgccccgc gcccgcgcaa cgcttcctgc tcacgcatct ccggcaatcc gatttgcgcg
    28921 cgcgcgtgac gctgcccgaa accgccgggg ccgcaggccc gccccgcctc atgttccgcc
    28981 agatcgaaac gcccgacctg cccgtacaag tgcggttccg gcgcgatgac gccggaacgg
    29041 tctatcccgt aacgcaccgc acgtggcgtc agatccccgc aacgtgccaa cccgaaatcc
    29101 tcgctctcgc ttccgccgcg ttcgccggta ccgcgcccac ggcccgcatc ccctggaagg
    29161 accgccacgc gaccgtggcc tgcgggctca cggaggtggc gtggcccttc ccgccggtgc
    29221 ggggtcactg gatggggatc gactcggccg gacgggatgt ccttgtgcgc gtgttgtacg
    29281 gaatgcgcac ggctatgctt ttcggcctgc tgttggcgct ctgggccgta ttgttcggtg
    29341 tggtgatcgg cgcagcccaa ggttatttcg gcggatgggt cgatatcctg ggccagcggc
    29401 tgaccgagat ctggagcgcg ttgccctttc tgtacgtgat gattttcatc ggctcggctc
    29461 tgggccgcag tttttcgctg ctcctgctct gttacggcct gtttaactgg atcggcatct
    29521 cctactacct gcgtgccgaa tttctgcgtc tgcgcaaccg cacctttgtg gaggccgcgc
    29581 gctgtcaagg gctctcgtcg gcccgcatca ttttcaacca catcctgccg aacgccttga
    29641 cgccgctgat cacgcttttc ccctttacgc tgatcggcgc catcggttcg ctcgccgcgc
    29701 tggattttct gggcttcggc ttgccgccgc tcacgccgag ttggggcgaa ctcttgcagc
    29761 aggcgcaaca gttccgctgg gcctggtggc tgattctctt cccgtcactg gccctgttca
    29821 cggtgatgct gctggcggtg ctgatcggcg aagggttgcg cgaggccttt gatccgcgtc
    29881 aggcaacgaa gctggagtag acatgttgct cgaaatccaa aatctccaga tccaatttga
    29941 gtcggacggc cagattgtcc gtgccgtgga aggggtttcg ttcgcaatcg accggggaga
    30001 ggtgctggga ctcgtcggcg agtctggctg cggcaaatcc gccaccgcga tgtgcatccc
    30061 gcgcctgctg ccggcgccgc cggcgcgcgt caccggcggc cgcgtcacac tggacgggcg
    30121 ggatctgctc gcgatgccgc ttccggagtt gcgtgccctg cgcggcgcga agatcggcgt
    30181 gattctccag gacgccatga cctcgctttc gccgctccac cgtgtcggcc gccaactcga
    30241 ggaggcggtc cggctgcacc ggcgggtctc ccccgccgcg gcgcgcgaga ccgcgctgga
    30301 ctggcttgac cgcgtgggca tccccgaccc ggccgcccgc gcccgcgcct acccccacga
    30361 gctgtccggc ggcatgcagc agcgcgtgat gatcgccatg gccctgctgc tggaaccgga
    30421 cctgatcatc gcggatgaac ccaccaccgc gctggatgtc acgatccagg cccaggtgct
    30481 gacgctcatg cgccggctcc gccgcgccaa cgcgggcatg ctgctcatca cgcacgacat
    30541 gggcgtggtc tcccagatgg ccacgcgcat cgcggtcatg tatgccgggc aaatcgtgga
    30601 acaggcagcg tccgaagcat tcttccgtac cccgcttcat ccgtattcga tcgccctgct
    30661 ggaggccatg ccctctgccg ccacgcgcgg caagcggctc aaggccattc cgggtcaggt
    30721 gccgccggca ggcaactttc cgccggggtg tcgttttcac gaccgctgtc ccgtcgcccg
    30781 tcccgaatgc gcccggacgc cgcccgcgct tcgggttctg gaagaccggt ccgtgcgctg
    30841 cccctatgcg ggtgcgtagg gtgccacagt gccccgtcta cggacgcttc ggggcgggcg
    30901 gaaagcggat tgcgggattg actcttttcg cctcccccgc ataatggggc gccgctcatg
    30961 ggaaccggtg tgttgttacc ggaccaacca acctgtggga tacgagtgaa tcgaagaaaa
    31021 gtgcgggtcg ttgttgacct gacccttcca tggagttctt aacgtgaaaa aacgaatcgt
    31081 gatcggtacg gccctcatat cgctcgcagg gtatgcgtcc tttcctccgg atgcggtgct
    31141 gtcggctttc ccgcgtcttg ccggtgaaca cgatgacgcg ccacgtttcc ggcgcgcgat
    31201 cgccgcggcg ccgaacgggg ttctcgcggt cgggaagggc gactattcga ttgcgacgcc
    31261 gctcgtgatc acgaaccgat gttcgctgga gatgcatccc gcggcacggc tcatcgccac
    31321 ggcaccgatg gagtttgtcc tcacttggga cggacatgat gattatcatg cgctctcgat
    31381 cttcaatccc gacggctcca tctatgacaa tgccggactc ttcatccgcg gcggcgatat
    31441 cgacggcagg ggtctcgcga gttgtcttcg aatcgggaac gcccatcact tcacgctcgc
    31501 caacatcacg ctccacaacg gacgcaaggc cggacttgaa ctctcgcgct ggaacggcgg
    31561 acacctctat gaactcatcg ccaacaacgt ctactgcaag tgcaccatga aggggcttgc
    31621 cggcaatatc ggcgtccttg ttcaagtcca tgactgtcat tttaccgaca tcatcgtcgt
    31681 tgattataca atcggcatcc gtgctacggg cagctcgaac cgtttcacgc gttgccatgt
    31741 ctggggcggc actgtcgcgc cgaaggggat gtccatgcgc gagtggtccg atttctatgc
    31801 ggccaacaaa aagcagcgca gcaggtggaa tgcggatctg gaagcggctc tcctgtcaaa
    31861 agggcttccg gaaatgctcg ccggatcgat ctgcttcgat tcctcctctg gaaatctgtt
    31921 cgacggctgc tatgccgata ccggggaaat cggcttcaac cttccgcatg gcggacagct
    31981 tgtgaattgc ggtttttaca acaacttact gatggggctc cgcaaatcaa cggtcatcgt
    32041 gcataaatcg gggcgtcttg tcatcaccgg ttgtaaattc aacggtgtta caggtgtcga
    32101 aaaactgtac gagggcccat ggaaggatgt ggtctggtcg tcctcgacag cctcgggcgg
    32161 cgcggacatg gacgccgacg caacgaaact ccggcatgga acccctgtgc agaagtgatc
    32221 gggttccggg ttccagccgg aagcgcggga atcctcaatc atctgaagat gaccagcgta
    32281 cccggtgaca ccacgtcgag gtagatcccg tccgggcgta tccgcacaaa gtatttgagg
    32341 taactcgtgc ccgtgtattg gacattccag ggacccgcga tggcggaggt gcagaacacg
    32401 agcggatagg atcccgtcgc gagtttggag atgtcgccct tgagggtcac ggtgccaccg
    32461 tcggaaaggt ctaccgcacc ggaaaccgtc agcggcgtga tcgtgccgtt gtcgttgatc
    32521 accacctcca gtccggcgcc ggaggcgaac gccaccgagc cgttaatcgt gccggcgccg
    32581 gcgagccgcg tcaccgtaag cggcatatca ccctccaccg tcaaggtcgt gccagcggcc
    32641 agcgtcacgt cttcggccgc ggacggcgcg tgacccaccg ttttgcgcgc aaaccacttc
    32701 cagttcagcc acgcatccac gacctgatgc gtgtagtacg agagcggctc ttcgtagatg
    32761 aggacctcgc ccagttcacc accgccgctg aattgacgcg cgttcgccat catgagcgac
    32821 tgaacgttgc accccgttcc cacggtatcg cggaaagaga ccagatcgta cccgccggaa
    32881 agccccgcgg tcgtgaaggt tgtcctcacg ccgttcaacc agttgccgcc ggaacggcag
    32941 cctgtcggcg cgttggaatt gaagaacggc accgccggat ctgcggtcgc agtgcgccac
    33001 tgtgctgtac cataacctcc caagggcaca ccgccgccgt tttgcgatcc gatcatcaga
    33061 aacacgcttc cgagggtggt caggttggtg taaaacatcg gagtgtaacc atccgtttcc
    33121 tgccagacga gatcatggaa gaggccccat ccgtagttga agtcgacggg cgaggtgttc
    33181 gtgagcacgc cgtaatcgac caccggcctg ccgccgagac tctccggata ggagaggtac
    33241 ggccccttgt accggtcgtc cgtagtgcgc cgctgcgcgg acacgggcga ggcgccggac
    33301 acgtcgcgcc agtgagtcac ctcgtcccgc gtcgcgccgt cgagatacgt tttcgaggtc
    33361 agggtcatgc ggttggtggc cgtcgcatcg agatgaacga acggtcccga cggaaccaca
    33421 tccgcggtca gttcctgcga acggatgacg acttcgcctt cgcggacgtc gagcgaagcg
    33481 ccgtccacga ggtccgcgta aagcgtgccc ccgccggttt tcatgagggt cgcactgttc
    33541 gtgagcgtgg tgaagcccat cacgttgccg gcagccacgt caacccccac atcgtgggtc
    33601 acgtccaaag cgtcgagatt cgaccggttt gtgaaacgcg aatcggaaag gttgtactcg
    33661 accgtcgcat tacggtactt ttgcatcagg tacgcgcaca cctgccgccg ctccgtcaaa
    33721 gtaagttcgt tcgtgtagac gatgcactcc atctggcgct ggcagccgga gcaggtgacg
    33781 ccggcgtgtt cctggatggc gaaggcatcg gcgcggccgt acggcgccaa aggatgtact
    33841 tcgagcacat acggcaccga gtacgcggcg ggcatcttcg cggcgagatc ccactcctgg
    33901 ccgtccacgt acagccagcc gttccggaca tgcgacggca caccggacgc gagaaggtaa
    33961 ttttgccatg tgccgacacc gccgccatca acggtggagt tgattccccg tccgaagtcg
    34021 ttgtagtcgc gcgccgtccc catctccgcg aaacgtttgg tgcagccgag aaccgcgttc
    34081 ccgccgctcc cataggcgta cacgacgtgg aacacggcgc agacattcgt gatcggctgg
    34141 ttccacacca tgccggattg cacagtccgg tagttggatt gcgacgcggt tgcgattccg
    34201 tcgtgccaca cgc
//
LOCUS       DAOSLE010000013        34114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1463_length_34114_cov_9.145336, whole genome shotgun sequence.
ACCESSION   DAOSLE010000013 DAOSLE010000000
VERSION     DAOSLE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 34114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34114
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1463_length_34114_cov_9.145336"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1357)
                     /locus_tag="PK576_01995"
     CDS             complement(254..1357)
                     /locus_tag="PK576_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE60392.1"
                     /translation="MKIKKRNQGGNGEGLGRRSFIGTIAGAAAALSAGCKATAAAKGQ
                     PAAASRAARRGAQRLSLDRLRQFEALGYGMFISYDIQAFYKGTIHGKVTDEMRRIPAS
                     CYAPDKLDVGQWIAVARDAGMKYAVLTAKRHPGFCLWPSRHTDYTVANSGNRTDVVEQ
                     YVKACAQCGIVPGIYYPAVDFHHLHGRPPQDDWRYVTSLHQTFITDQITELLTEYGEI
                     GEVWIDIPKVLGRGYRTFLYEHIARLQPNAVILMNSGIQDGLKINVEKVWPTDLISIE
                     VKGPAKGGYQKWRTVEGREYYLPGEYCNSIMPKWWWREGDLPRPDQDVLKEFQAARAV
                     GVNVLLDAPPDNHGLIPKETVDALMRLRRNAGL"
     gene            1543..2106
                     /locus_tag="PK576_02000"
     CDS             1543..2106
                     /locus_tag="PK576_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60393.1"
                     /translation="MAQEWNIRPRGHVCSICARPFEDRKPCVSVLRDVDGTYERFDCC
                     PACWRDTPREWEPFSFWEGEYEAPAPITPKQEVVKRETAEGLLRRLITLEDPAMRDAV
                     YVLAVMLERGRQLVERDAKPHESGGILRVYEHKKSGDTFVVLDPRLRLDQLGAVQQQV
                     VALLSGTHALDVQPETAAPAEPVSETE"
     gene            2117..3739
                     /locus_tag="PK576_02005"
     CDS             2117..3739
                     /locus_tag="PK576_02005"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOE60394.1"
                     /translation="MTFDLILRGGQVADGSGAPARPADVAVKGDRIAAVGDLASARAT
                     REIQAAGLMVAPGFIDAHAHSDAYLLIEPDAPSKLTQGITTEVNGQCGGSVAPRLGEA
                     RLSSDWASQHYPRFSPAGGVEANPDPGPTWGTVAEYRRLFDAVQPAINTIQFIGHNTL
                     RAGVMGYAPRPAAAADLREMIRRLEQALEEGGWGLSTGLLYPPGKYAAGEEVAALVRA
                     AALRGGMYATHMRSEGDELLESVGEVLRLARDTGIRVQISHLKTSGRDNWPKIEALLE
                     MLRRARDAGVALHGDRYPYTASGTDLDVVLPEWAAAGGRDAILANLRSPAARRRMEEE
                     LDANPRDWSAVMIGGGWSAEVRRFSGRTVADAAAELGLSPGSVVCRFIERDETRTGAF
                     FFVMCEANLRHLYREPWIMPGSDASLRAPWGPLGLDHPHPRAYGTMPRFVRIVTGREE
                     GVPAVCGIEEAVRRMTSLPACAFGIRDRGTLIPGAFADVTVFDPETFRDTATFAVPHQ
                     FAAGVRQVIVNGAVAFDNGRFTGERRGRFLSR"
     gene            complement(3794..4831)
                     /locus_tag="PK576_02010"
     CDS             complement(3794..4831)
                     /locus_tag="PK576_02010"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOE60395.1"
                     /translation="MKNKKPARRNARITQVAETLRAAKRVLITGHVRPDGDSLGGMIA
                     LAHLLNRAGVRAVATAARTGLGGPGFLTGVKKLLAPEEAVKRSFDLMVALDCGAFDRM
                     PEALHPLASRLPLINIDHHLTNTLFGTLNWIDGRASSTGEMLWRLARKAGWPLDTVSA
                     EALWVALITDTGRFAHEQTRPATLRCGADLLRYGVRTAFINDKLYCSFSRVAMELKRR
                     AFRTLTVWGEDNTVALVTLTGQDFEETGGTKADAEDVIEIPRSLVNNRVALFFYGSEE
                     DNAETRVSIRTRRPLDATVLAKQFGGGGHSCAAGCTIRQPLSQAKRLFRKAVADLLEK
                     NPSDDASLSSS"
     gene            complement(4839..6116)
                     /locus_tag="PK576_02015"
     CDS             complement(4839..6116)
                     /locus_tag="PK576_02015"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017941110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOE60396.1"
                     /translation="MTPNLNEPNLAETLRLIGDQAQAAARRMVGLSTRRKNAILNAMT
                     EALEARRDQIREANDRDLEAARSAGIPAALLDRLTLTGARIDAFIQGVREVALLPDPV
                     GRLIWRRNRPNGLRIEKRRVPLGVVAIIYEARPNVTADAAVLCIKTSNAVILRGGKEA
                     FHSNLAIARALQEGGERAGLPPHAVQLIETTDRGAVRELVRLEDQVDVVIPRGGESLI
                     RAVMEHARVPVLKHYKGVCHVFVDASADLAQALAIAENAKCQRPGVCNAAETLLVHRD
                     VADAFLPQLGELAARRGVTLRGCPATRAVLPACEPATEADWTAEYLDLILAVRVVRDL
                     KTAIAHINTFGSHHSDAIVTADTRSANRFLKEVDSAAVYVNASTRFTDGGEFGMGAEM
                     GISTDKLHARGPMGLEELTTYKYRITGTGQVRG"
     gene            complement(6130..6936)
                     /gene="proB"
                     /locus_tag="PK576_02020"
     CDS             complement(6130..6936)
                     /gene="proB"
                     /locus_tag="PK576_02020"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOE60397.1"
                     /translation="MNVEHRYREFLPEARRVVVKIGTRVIAHPSGRPDMRPLKGLVRQ
                     IARLHRKGYELLVVSSGAIGAGMEALGMTVRPTSVPDLQMCAAVGQARLMAHYEELFD
                     TEKITIGQVLLTHADFQHRIRFTNAKRTLEHMIRRDVIPIINENDAVADEEIKAGLSL
                     GDNDYLAALVVKLVRADLLVILSTVDGVLDAKNRRVACIENLNEAFALVMPTEEGGLS
                     KGGMDSKLRAAQIAVRAGCSVVVACGRTPDVLTQVMEGCDTGTLILSSTL"
     gene            complement(6954..7793)
                     /gene="tsf"
                     /locus_tag="PK576_02025"
     CDS             complement(6954..7793)
                     /gene="tsf"
                     /locus_tag="PK576_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE60398.1"
                     /translation="MAEITVAAINDLRQRTNAGMMDCKKALTDANGDIDAAIKALREK
                     GLAIQVKRADKESKEGIIMARTSEDGKTIGMAEVNTETDFVAKTDNFKQFVKAVTDKT
                     LEGVEDVSAALKDELMTIVSQTGENVKIRRTALYKLSGTGKVASYIHMGGKVGVLVEV
                     GCGNEATATSAAFAELVHDLTLQIAAAAPRWITPDEVPAEVVEAEKDIYRNQVQGKPA
                     NIVENILKGKIQKFYSDVCLIHQPFIKEPKQTVTDVVNACAKATGDTITIKRFVRYQL
                     GAA"
     gene            complement(7870..8877)
                     /gene="rpsB"
                     /locus_tag="PK576_02030"
     CDS             complement(7870..8877)
                     /gene="rpsB"
                     /locus_tag="PK576_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE60399.1"
                     /translation="MDMPVSSLTGLTLKDLLDAGLHFGHQTKRWNPKMKRYIFDKRNG
                     IHIIDLTQTVTLIDEAAAFLREAVLSGKKILFVATKKQAQETVREAAEACGQFHMTER
                     WLGGTLTNNQTIRRSVRRMRQIEAMGRNNNGVLSVHKKEASSLRRELEKLQKNLGGVA
                     DMESKPAALFIVDICREQNAVKEARRLGIPLVAIVDTNADPDPVQYVIPGNDDAVRGI
                     KLIADAFAKVIKNANDEYSRIAAERQQQKEAELASQEAQERAARKAARKKEAAGAEGK
                     ADKAKTKLAETRKRAAKVAKETVKAKAAAVEAAAPAPEAAAPAVVEAAPAAEASAPAA
                     E"
     gene            9275..9901
                     /locus_tag="PK576_02035"
     CDS             9275..9901
                     /locus_tag="PK576_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE60400.1"
                     /translation="MIKRQKTPTRHGADGKKSAAPKTEAPQQPDAQPAAAAQPPAAPE
                     PDQSPPEAQELATLKDRYTRLLADFDNFRKRQARDNEEFVRRANENLLSDLLPVVDHL
                     ELALAQTPDPDDPFVIGVRLVYEQIQSLFGRYGLTPIDAKGEPFDPAFHEAISQASSL
                     TVPTQTVIEQFRRGWMLSGRLLRPAQVVVSSGKPEPEQVEAESVSVSD"
     gene            9960..11153
                     /gene="dnaJ"
                     /locus_tag="PK576_02040"
     CDS             9960..11153
                     /gene="dnaJ"
                     /locus_tag="PK576_02040"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE60401.1"
                     /translation="MATQRDYYDILGVGKKASAEEIKKAYRKLAMKFHPDRNPDNKEA
                     TEKFKEVCEAYEVLSDTDKRQRYDQFGHEGMKSAFGPGGFDFRRDFSHMQDIDLQDIL
                     GSFFGGGFGDLFGGNGGGRRRSRNPNAPQRGDDLRFDLEIDLEEAIFGSEREVDLSVN
                     DACPTCKGRGTTSGSGRETCRQCDGHGVVIVGGGFIQMRQTCPVCRGEGTIIRNPCKP
                     CGGSGRVKVPRHLALRIPKGVETGSRLRLSGKGESGLRGGPPGDLYVVLRVRGHKLFQ
                     RQDDDLACTVFVSPVTAILGGDIEVPTPDGHARIKLPAGTPNGKVFRLRGKGMPNLNG
                     GTGDLHVRVEIEVPVRLSSRQKKTLEEFASQTTVENFPETIKQNKAIDTFFARRDALR
                     KHTST"
     gene            11227..11961
                     /locus_tag="PK576_02045"
     CDS             11227..11961
                     /locus_tag="PK576_02045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOE60402.1"
                     /translation="MHRCLVTTENLLADTLTLGREASHHLHTVLRVRPDDPVVLFDGQ
                     GRTRRALVRSAERHSVALTAAEPAQTHPAPVCAITLFACISKGHRMDWTVEKAVELGI
                     ARLVPVISEHTVVRLAPGDREGKAERWRRVAAEAARQCGAVWMPTIVSPLPFREALPL
                     VATTLPVFTAALTPAARPLREVFASHSEPPVHAGWFVGPEGDFSEAELAELTATGATP
                     VSLGPLVLRSETACLYGLCALNAAWL"
     gene            12021..13376
                     /locus_tag="PK576_02050"
     CDS             12021..13376
                     /locus_tag="PK576_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HOE60403.1"
                     /translation="MNQYVRFDSVGGASGDMILAALAALGADLDSIGQTLRSFFPQPL
                     FLHTEPAAAAGLHGLRVAVRCDDSEDHDPHVWPDAHTPAHGPDSGHSHHHRPTPQPGH
                     PHGHTHGHTHGHTHNAGHGAHSHTHRGLREIEQLLASAPISDAARTLTRDVFRALAEA
                     EAAIHGKTPETVHFHEVGAWDSVADIVGACLALEQLGIRGVACGPLPAGTGTIHCAHG
                     EMPNPAPATLELLAGMTVTQTEEPFELVTPTGAALLRVWTRTLEPVPREATVLRSAFG
                     FGSRTLQSRPNVLRATLLEAAAAPAPEPEERLVLETNLDDCNPEWLGALTTELLECGA
                     LDVWQIPATMKKGRAGVILGVLARPADADVLRERIFRGTTTFGIRSHAVRRETLSRRF
                     VTAHTPWGEVPVKLGALRGETLTATPEHDACERLARTHGVTPRQVCEAARQSAGEPKR
                     E"
     gene            13373..14512
                     /locus_tag="PK576_02055"
     CDS             13373..14512
                     /locus_tag="PK576_02055"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE60404.1"
                     /translation="MKNPESVFKQPPHVVQLLPALDEGGVERGTVELNREFVRHGLRS
                     TVVSNGGRLVERIERDGGRHCRLDLKSKNVLSAPARAGTLRRLLSELRPDLVHIRSRV
                     PGWLFAWANRTLGLPHVSTVHGLNSVNAYSRIMTAGVRVICPSSAVANHIRAHYATPE
                     ERIRVIPRGLDPQQFDPQRLDTAFMAAFRDQFGLAGRFVVLGVGRLTPLKGFDVLLHA
                     TDLARKRMPQIKTVIVGGAETAREGYARELYRLAESLGLDDHVAFAGSQSRMAEIYAC
                     GRVLVSGNHAKPEAFGRTMTEALAMECPVIATRFGGALDIVREGVNGWLVEPGDPAAL
                     ADRLVTASQTELTGLREDALERFSLEAMVRATLAVYAEVLGAESP"
     gene            14546..15820
                     /gene="purD"
                     /locus_tag="PK576_02060"
     CDS             14546..15820
                     /gene="purD"
                     /locus_tag="PK576_02060"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOE60405.1"
                     /translation="MNILVIGGGGREHALAWRLAQDSCRPKLFCAPGNAGTAAFCTNL
                     PLAAEDLDGLVAWSRENRPDLTVVGPEIALCLGITDALEAEGLRVFGPCRAAARMEGS
                     KCFAKEVMRAAGVPTARSASFRSSAAARAALDDFALPVVIKADGLAAGKGVIIAQTRQ
                     EAEAALVSMLEEAVFGKAGAEVLIEEFLEGEEASILALVDGEHAVLLPSSQDHKRVSD
                     GDQGPNTGGMGAYSPAPVVTPELLPVIRDTVILPVVRELKRRGIVYKGVLYAGLMIGP
                     QGIRVLEFNARFGDPETQAVLPRLGGDLIPALEACINGTLSDSLLTVRPEAAATVILA
                     SGGYPGAYRKGLPVTGLDQAAALDDVLLFHAGTALRDGQIVTAGGRVLAVTALGTGLR
                     AAVGRAYEAVGKIHFEGLHFRTDIAHRALDRE"
     gene            15882..16382
                     /gene="purE"
                     /locus_tag="PK576_02065"
     CDS             15882..16382
                     /gene="purE"
                     /locus_tag="PK576_02065"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOE60406.1"
                     /translation="MPTPLIGIVMGSDSDWPLVQKAADTLNAFGVAFEARVISAHRTP
                     DLAFEYASSAETRGLKVLIAAAGGAAHLGGVLAAHTALPVVGIPIPGGALNGLDALYA
                     TLQMPAGVPVATVTLGNAGPVNAALLAVQILGTADPELRAKFRAYKEELKRKVLEGDA
                     KINAAL"
     gene            complement(16419..17528)
                     /locus_tag="PK576_02070"
     CDS             complement(16419..17528)
                     /locus_tag="PK576_02070"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016861193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOE60407.1"
                     /translation="MELSLLMQAFDVSGRLVTINPTGNGNVNDTFLAIFRNTFAEEQV
                     ILQRVNPLVFPEPQIIMQNLHRLTEHAHRKLETEGDTTDRVWQMPRIVKTRDGQDYYV
                     DDAGEVWRVITKIASATAFDEAQNAEHAAECGAVLGHFHGLVSDLDPRSMTDPLPGFH
                     ITPGYLAKYDAVLESEPLAKKRLGASTEARRMAMFIEERRGVAATLQRALEAGELTLR
                     IMHGDPKVNNIMIDDFTGKGTAIIDLDTVGPGLIHYDFGDALRSICNHAGEDEQNLSK
                     VVFDLDLCEAFCKGYMAHAGEFLTDTDRYYLYDAIRLITFELGLRFFEDYLAGSVYFK
                     IRHPEQNLNRARVQFRLCESIEARERAIRNLLAAL"
     gene            complement(17592..18611)
                     /locus_tag="PK576_02075"
     CDS             complement(17592..18611)
                     /locus_tag="PK576_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOE60408.1"
                     /translation="MRTGLWAWTAIVLGIGLSSPLFGDEVLFKSGDRLTGKVEKVADG
                     KMTFKSNVAGPLVLNMEDIKTFSTDEPIELALADGSLQKQKVAASDEGYVTIAGTAQP
                     ASLPIANIAKINPDKPRWKGVVGAGATLVRGNTKSDTASVTAEAARRGENDRISFGAG
                     YYFAKQRDNSTRDDSTIADNWFLKGQYDYFFSKQFYGYGNLRYEKDRIANLDIRLTPG
                     LGVGYQWIEREDFNFSTEAGANWVYEKYTEPDETRTYMAGRLAYHVDKSFNDRVKGFH
                     NLEYIPSFERADTFLVNADVGLRAAITARMAVEAKTQLAYNSKPSEGRDKKDLRHILG
                     VAWTF"
     gene            18862..19458
                     /locus_tag="PK576_02080"
     CDS             18862..19458
                     /locus_tag="PK576_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60409.1"
                     /translation="MSGGTSHPDAGKRCGAVLMETVLAIPLFMIFLGGVMWVGDLLVT
                     RQQLVIADRFVAWNHGLRYPDKEKTDPGTIHRYFFTEANGAPSPYHQPSSSDARIDET
                     FDWSHAASGQVRLQMRMPQWVRSMFNAGQVFYDSGVPLEQASNLFGRDKEDQRHVVVM
                     RTRREAQADYIRNRYGRDKSAEVAEQWEAISDEAWPYE"
     gene            19436..19813
                     /locus_tag="PK576_02085"
     CDS             19436..19813
                     /locus_tag="PK576_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60410.1"
                     /translation="MRRGPTNSLACAALGLLGLALAAAGAPQAPLPRLPLPPLATVLR
                     EDLNSRTWRQEGELSGTLENASREFRQALAGGGWRLEKRIVAGRSHLLLCSRKRTRFL
                     VMVWEKGVGACGFAWGEDAQQGP"
     gene            19828..20631
                     /gene="cpaB"
                     /locus_tag="PK576_02090"
     CDS             19828..20631
                     /gene="cpaB"
                     /locus_tag="PK576_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOE60411.1"
                     /translation="MKNFIPLILAVLLGLAAVLAVGRLLQARKQAAEKMTSVVAVARD
                     IAAGDVLTADSIMEKKIPVSARPAQAIFWSRSEMVKGQKALRSIAQGDYVLLSDVGLS
                     RSMADIVGEGEWAVALTLPPGGIARLVQPGDEVAVIGTFRIQSKVVSADLSAAPAEVA
                     KEATLVLFPRVRVLDVATDRAGGDEAAGELILALPPQQAQVLVAAQREAQLTLALRRP
                     GDTAAINRMDIGMVDAETFDKLLKGLKPVEMPTIPGQIDAAGSGKPAGQ"
     gene            20650..22224
                     /locus_tag="PK576_02095"
     CDS             20650..22224
                     /locus_tag="PK576_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOE60412.1"
                     /translation="MSTFRNRMRVITSAMAFAVFALVMSPALARAADAEPVTVTVGKM
                     EVINVAFPIVGFRMADASVAKVEQQGQQQLRVMGLRAGTTDLQVAGEGNVSAIFTLTV
                     TDNINAVLAAVKRDLDTVPEVDVAINLGRVLIKGEVSNIEHWNYLQKVVALYKDNVVN
                     LASFRPAPEVMLGLKAAFEKAGVKVLGTEAPAESAEPGSVKLAFSGNNIFVSGQVYSQ
                     RDKDRILQIIEAQNWITFKRGGDEKQDATKPVNEGAVRALVDLTIVPTMIEMEAVFVG
                     VTDAEEKQIGVNLAKAGLLVLDSTSAGFQGDLATRSSGWAGTYTINSGLQGALKFFAG
                     NGPGRFQNAGHMTFKNDSPEWRTYHSGGTLKVRVATRDATSLEDVDYGLLMKVKGGLL
                     NAKAAALDVNLELSYPVPVGNDYDLKRNRIETTVNCPIGQTIVLAGMKSLVEQSNMEG
                     VPFLRSVPAISWFFSEKNTRKEDSKVLILMSPRIAGMPIETPAVSEQTAPALQESDKP
                     VQQRQLEKQKRRFFFF"
     gene            22231..23910
                     /locus_tag="PK576_02100"
     CDS             22231..23910
                     /locus_tag="PK576_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE60413.1"
                     /translation="MATLTLIPSGGSGEAEVYTLEPAGVTTLGRSPENRIVLHDNRVS
                     RAHLTVTGKGGVFRVRDLDSTYGTLLNGKRLTTEATLREGDSLQIGGFSLRFGQGTKE
                     TPSETPAADAQHAAEPDIEMDPALAAMAALYTEEMMTMKRRIHEQVLFKLNLPEIATK
                     QIQDEEMRAKLENALDQVLREVRHELPRDLINNDLFRQALLDELIGYGPITPMLRDPT
                     VDEVMVNGPARIFVERKGVLYETGMRFFDDRHLITIIQRIVEPLGRHVDEASPMVDAR
                     LPDGSRVNAIIPPLALDGSSVTVRKFAKRKLTTDDLIAFGSMTPEIALFLEEAVRARQ
                     NIVVSGGTGSGKTTLLNVLSQFIPRNERLVTIEDSAELKLSHRNIVRLEARPANIEGR
                     GRVAIRDLVINALRMRPDRIVVGECRGAEALDMLQAMNTGHDGSLTTAHANTPRDCLA
                     RLENMVMMAGFELPSAAIREQIASAINLIIQQNRLADGSRKIVTISEVTGREGNTILL
                     QDIFVFEQKGFSPEGKVLGQMVATGNIPQFVEALKQRGDLRLDMGVFVPKA"
     gene            23942..24823
                     /locus_tag="PK576_02105"
     CDS             23942..24823
                     /locus_tag="PK576_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE60414.1"
                     /translation="MTDILVLVLVFAATTWGVWVVTPLLAQIGLERSATQHIRSDGVK
                     QLGPIYRFTTPERLAQACWSAALLGAGLAAAALIAFNVLSPMLIGGACLLAGVIAFHI
                     PAKWVQHRIRTRQRLFDARLTDLTLGLANGLRAGAALPQSLEIVSRDMGGPMTEELML
                     VLQEYRLGMDLPEALSRLCERMPGEDLSLLVTAIRLTMQSGGSLAEVLDRITDTIRQR
                     TEFKDRLLTMTAQGRFEAIAMAAAPLVAFLILFFLDRELMQPLLQTKIGWCAIGAVVI
                     LETVGFLVINKIVTIDV"
     gene            24835..25734
                     /locus_tag="PK576_02110"
     CDS             24835..25734
                     /locus_tag="PK576_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE60415.1"
                     /translation="MDVIIIQMLTFLSVLFLVNGIQAVIRKENETLSNAKPLVFRVFH
                     HEIGTIGRATGAWIDQTFPVQTRQIRNDLIAGAMPIESVEVRGLQGFAGAVLFAVAGL
                     TTFILTLNWAYGLLAGIGFGLMGWYYPVAWVSRCARTRKDLMSKSMPYAIDLITVSME
                     AGQDFGAAVRNLVHDGPKGPLRDEFGVTLRQIELGKSRVEGLKAMADRVQLDEFRSLV
                     TSVVQSSEMGASISATLKIQAEEIRRRRYQRAERQAARAPSLMLIPTALFILPAVFII
                     IATPIIIRAIESGVGEYFQSGGG"
     gene            25740..27413
                     /locus_tag="PK576_02115"
     CDS             25740..27413
                     /locus_tag="PK576_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE60416.1"
                     /translation="MFCLKVEKGEPAGEVHILKEGENTLGRSRSATFRLGASDVSGQH
                     ARIVVRGGVVTVENLSQFGTKVDGTPITGSVQLTEGQRLSMGESTVLCLLRTPAGEPS
                     AGADMSAAEPDVTQTKGTLGVEAILQARADEIPTGEGVPPSPETGGATRATPPPPPPP
                     AQVAAAAPTGAEFGAPVPAYPDSDLTSGLPLEALTVDSETIEGATRAMQTRAATPEEI
                     EHLRIVEQKRHKKRITRALAVLIPLIALVVIFRPRTPPPEPKIEWAKDEEGNYLDLFE
                     AAPSGGLAEGGYDLCYPGPVRFDKTVTEDGVILSGFVGRERNVPMRLITQEQTDPKFV
                     TMDRLAFVEEWIQQQKADNKRWSFDKPSPMPGFIGIRNGIPYLRVTYYRDDDEGSWFG
                     VASVFRYGYRRIVIRAEAPASERVRLEDLLSSKFMRASEAMEYAYWEAPEELPKQSEA
                     VLLKQAEADLNRMAPATWFELQRNLSGLLARTVLSGNKTTEVEALRLLVRLRERLTLW
                     FNSQQLAFDAARMQNNRGQARETARLTRAVFKDMEDQRYYTVRKWNTEF"
     gene            27418..29715
                     /locus_tag="PK576_02120"
     CDS             27418..29715
                     /locus_tag="PK576_02120"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE60417.1"
                     /translation="MLIRLEHNGRIIHEIGSDALTAELTIGRSHACEWPIPKEDGVAS
                     SRHAALFKKGRAVWVKDLKSTNGTFHNGKKIEKRKLAIGDKISIGNCVLSAEADRSGT
                     GRNLSEVVVLSGKTRGQKKRLVPPVFTIGSDPSSSLVFLDMLVSRRHAEILIREDGSC
                     WIRDLGSKNGTSVNGMPLRDDKERLLKDGDCIACSHFELEFRDGAVKHSNKQTWLRLG
                     ILIVTLLAGLGAYHTYQRLKPSAEAFVKAARRLAAKEEFDQAEREVEQAVNARHASRH
                     QVAIEEMRRLIGVWKRTRALWQSAKEDLERGEWVGASRTLGVLQASKREVWEWNAQAA
                     EAKDAAAYAKTLLDAFLQTDALLKREDLEFAELENNQPVLDRVLDEKPATAEPAAYLK
                     RLLDELRRVQTRQTALLTESRQMETALTKLGEAQPSYDEVVSLLEQLATSQEPALRRR
                     AQVLVVPVKALANSYIKLGEAATTLRSLAFQSALKLDLALPTVNACAIEPRVSLARQN
                     IEKIHANLKLKGYQLDSLFTEVEKRIGRNDDSLEGFRVLRNSAMMEKVMACDSMNLPL
                     PKRSRSQPAGEYDRLLGIEAFYAYLSAQPEPLDPAMLADLPFVPLINQTREALQKIDT
                     FLQFMAQADNQWLLDDALAVQVKRMKAIMQQRDELVKLLITRAGSATDRTALIAGGLA
                     ARLITVSGKTKIKDLPPEEWLAAELKRQRTALLRMNEEYTLAAPTRQIEIRAAVLKAG
                     LPGDPVVRRMWAMRDAAAAAMSSTP"
     gene            complement(29760..30347)
                     /locus_tag="PK576_02125"
     CDS             complement(29760..30347)
                     /locus_tag="PK576_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOE60418.1"
                     /translation="MTAITFGRVPFVLLFMVLAVTHAYMAPRGVWLAVAATASLSIAA
                     LTDLCDGKLARKWQVVSRFGAMADPLMDKVFYLVVFPTLLWLLGRQPNEAEHALVMLI
                     FTVLYILRDQWVTFLRSVGAMFQADCRANWMGKFRTAVSFPAACVIYVYASLHPWFLP
                     QALIYLIEALLIILNLWSIVVYTRQYLPYIKQALQ"
     gene            complement(30395..31834)
                     /gene="uxaC"
                     /locus_tag="PK576_02130"
     CDS             complement(30395..31834)
                     /gene="uxaC"
                     /locus_tag="PK576_02130"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOE60419.1"
                     /translation="MKPFIHDDFLLDNAAARELYHGYAATMPILDYHCHLPPAEIAED
                     RRWENMTRLWLGGDHYKWRALRSNGVDERFITGDAPDREKFQKYAETLPYLLRNPLFD
                     WSQLELARYFGITDLLTPATAEAIWTETGRQLAEPAFSARGLMVRSNVRLVCTTDDPA
                     DSLEHHAALRAENFAVRVLPTWRPDKALALDRPAFWNAWLDRLSAAADMPVKTWDDLL
                     CALQRRHEVFAAHGCRLSDYGLDTVYADPYLDTEIREIFHRVRQGGKATPEEVSAFRS
                     AFLYESLTLDAKSDWSAQIHYGAMRNNNTIMFKRLGPDTGFDSIGDWHVASPMARLFD
                     RLEQAGALPRTVIYTLNPRDNEMVATLLGNFQRGPEPGRMQFGSGWWFNDQRDGMLRQ
                     MEALSQLGLLSRFVGMLTDSRSFLSYTRHEYFRRVLCNLLGTDITQGRIPNDLAWTGE
                     IVKDICTRNAERYFGFDLPDAPVLSFQER"
     gene            32144..32698
                     /locus_tag="PK576_02135"
     CDS             32144..32698
                     /locus_tag="PK576_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE60420.1"
                     /translation="MAQLSITFCRKLARARRERGMTQSALAQAVSCAQSAISMLESGQ
                     PGKLSQETVGKIASLLGVPLEDSAPDGVPLPTRPSAGYCPNAACFSNIPYVVADEVLF
                     WPRPQPTAARGPYCVYCGEVLEPQCPHCGAGVTPGACCPQCGRARITGGAPDGVDPED
                     WAARRRRELADWRALSAWTGFASE"
     gene            32742..33683
                     /locus_tag="PK576_02140"
     CDS             32742..33683
                     /locus_tag="PK576_02140"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HOE60421.1"
                     /translation="MTKKLLIVTDGKAGHENQSKAFCAALGYGYECVRAVYPSRFHKG
                     LSYLIDWLGLLLSSPFTIEPTDGYFAAVVCTGSTAFYPGKIEARRRGIPVAAILYPSG
                     YRKANFDCILAPSFDRPRPAPNVIPIPVNLTSTDAAFYETGVAAFRARHTQRKPASAL
                     IVGGPNAAAELSVETLRRDLDRFFAVTEGNERWVTTSRRTPPAIEEFIASRPFDYSLL
                     YSRDTFNPIPAFVSLCEHLFVTADSTGMISEAVTRGRARVEILMTLRKPDSKFGRFIR
                     DLEQAGAVHVFDGTPGCADRKIDLAPAFEQARKLLGL"
     gene            complement(33916..>34114)
                     /locus_tag="PK576_02145"
     CDS             complement(33916..>34114)
                     /locus_tag="PK576_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60422.1"
                     /translation="NRERAVERNCSFFYPTIDSDPDSDSDPDSDSDSDSDLDEAMKHM
                     KLHENQEFPLAFQKNPSSAIP"
BASE COUNT         6265 a        10595 c        10924 g         6330 t
ORIGIN      
        1 ggaattcccg attttcatga agttttatgt gtttcatggt ttcatccaaa tcgggatcgg
       61 gatcgggatc gtcgacccgc gacattcgac ccgcgacgat cgacagggac gcccgcgttt
      121 ccgacaccgc aggtcgctcg tcgcgggtcg cgggtcgaca taaggcctga cgggagaagc
      181 gggcgtccct accccgcgtg gcggggcggt tcccccgaaa ccgccgcggg cgcggcacac
      241 gccctctgac cgctcacagc cccgcattgc ggcgcaaccg catcagcgcg tcaaccgttt
      301 ccttcggaat caggccatgg ttgtcgggcg gcgcgtccaa caggacgttg actccaacag
      361 cgcgggcggc ttgaaattcc ttaagcacat cctgatcggg ccggggcagg tcaccttcgc
      421 gccaccacca tttcggcatg atggaattgc agtattcgcc cggcaggtaa tactcccggc
      481 cctcgacggt ccgccacttc tggtatcctc ccttagccgg gcctttaacc tcaatcgaga
      541 tcaaatccgt gggccagact ttttccacgt tgattttcaa accatcctga atcccgctgt
      601 tcatcaagat caccgcgttg ggttgcaaac gcgcgatatg ctcgtagagg aaagtgcggt
      661 aaccgcgtcc gagcacttta gggatgtcaa tccagacttc gccgatctca ccgtactccg
      721 tcagcaactc cgtgatttga tccgtgatga aggtctgatg aagcgaggtc acatagcgcc
      781 agtcatcctg cggcggccga ccgtgaagat ggtggaaatc cacggccgga tagtaaattc
      841 ccggcacaat accgcattgc gcacacgcct tcacatactg ctccaccaca tccgtccggt
      901 ttccgctgtt ggccacggtg tagtcggtat gtcggctggg ccagagacag aatccgggat
      961 ggcgtttggc cgtgagcacg gcgtatttca tgccagcatc gcgcgccacc gcgatccact
     1021 gcccgacatc cagtttgtcg ggcgcataac atgacgccgg aatgcggcgc atctcatccg
     1081 tgaccttgcc gtggatggtt cccttgtaaa aagcctgaat gtcgtacgag atgaacatcc
     1141 cgtagcccag cgcctcgaac tggcggagcc ggtccaagga gagccgctgc gcaccccgcc
     1201 gggcggcacg ggaagcggcc gcaggctggc ctttggcagc ggcggtcgcc ttacaccctg
     1261 ccgacaaagc cgctgcggca ccggcaatgg taccgataaa actacggcgt cccagccctt
     1321 cgccattgcc gccctggttt ctctttttga ttttcatggt tttgattgta tccaaaacat
     1381 cgagaaagaa caaccttact gattccgggt tccggttttt gtaccatcaa aaagtcacgc
     1441 cgccggtttt ggccgatgcg ctctttgcgt taaaaaacgc cccgcatccc gatttctaaa
     1501 cgcgcgggtg tctgtccgcg cgttttttgt tatactgccc ccatggcaca agagtggaat
     1561 atccgcccgc gagggcatgt ctgttcaatc tgcgcccggc cgttcgagga ccggaagccg
     1621 tgcgtgtcgg tcttgcgcga tgtggatggc acgtatgagc ggtttgactg ctgtcccgcc
     1681 tgctggcgcg acacgccgcg cgagtgggag ccgttcagct tttgggaggg cgaatacgag
     1741 gcccccgcgc caatcacgcc gaagcaagag gtggtgaagc gggagactgc cgaggggttg
     1801 ctgcggcggc tgatcacgct ggaggatccc gccatgcggg atgcggtcta tgtgctggcc
     1861 gtgatgttgg agcgcggtcg ccaactggtt gagcgggatg ccaaaccgca tgagagcggc
     1921 ggcattctgc gcgtgtatga gcacaagaag agcggcgaca cctttgtggt gctcgacccc
     1981 cggttgcggc tcgatcagtt gggtgcggtg cagcagcagg tggtggcgct gctcagcggg
     2041 acgcacgcgc ttgacgtcca gcccgaaacc gcggccccgg cggaaccggt gtccgaaacc
     2101 gaatgaggtc tcccgcatga cctttgatct catcctgcgc ggcggacagg tggcggacgg
     2161 aagcggtgcg ccagcgcggc ccgcagatgt cgcggtgaag ggggatcgta tcgcggcggt
     2221 cggggatctt gcctctgcgc gggctacgcg ggagatccaa gccgcgggcc tcatggtcgc
     2281 gccgggattc atcgacgcgc acgcccattc ggacgcttat ctgctgatcg aacccgacgc
     2341 tccgagcaaa ctcacgcagg gcatcaccac ggaggtcaac ggccaatgcg gcggatcggt
     2401 cgcgccgcgg ctgggtgagg cgcgcctctc ctccgattgg gcgtcgcaac attacccacg
     2461 cttctcccct gcgggtggcg tggaggcgaa tccggatccc ggaccgacct ggggcacggt
     2521 cgccgaatac cgccggttat tcgatgcggt gcagccagcg atcaacacca tccagttcat
     2581 cggtcacaac accttgcgcg cgggggtcat ggggtatgcc ccgcgcccgg ctgcggcagc
     2641 ggaccttcgc gaaatgatcc ggcgattgga acaggcgctg gaggagggcg gatggggatt
     2701 gagcaccggt ctgctctacc cgcccggtaa atacgccgcc ggggaggagg tcgcggcact
     2761 ggtccgcgcg gccgcgctcc gcggggggat gtacgccacg cacatgcgca gcgagggaga
     2821 cgaactgctg gaatcggtcg gcgaggttct gcgtctcgct cgcgacaccg gaatccgtgt
     2881 gcagatctcg cacctgaaga cctccggtcg cgataactgg ccgaagatcg aagcgctgct
     2941 cgagatgctc cgtcgcgcgc gcgatgcggg agtggcgctc cacggtgacc gttacccgta
     3001 caccgcatcc ggaacggatt tggatgtggt gctgccggaa tgggccgcag ccgggggacg
     3061 cgacgcgatc ctcgccaacc tccgttcccc tgcggcgcgc cgacgcatgg aggaggagct
     3121 cgacgcaaac ccgcgcgact ggtcagccgt catgatcggc ggcggctgga gtgcagaggt
     3181 gcggcggttc agcggacgca ccgtggcgga cgcggcggcg gaactgggcc tttcaccggg
     3241 cagcgtggtc tgccgcttca tcgaacgtga cgagacgcgc acaggcgcgt tcttcttcgt
     3301 catgtgcgaa gcgaacctgc ggcacctcta ccgcgagccg tggatcatgc ccggtagcga
     3361 cgcctcgttg cgtgcgccgt ggggaccgct gggcctcgac catccgcacc cgcgcgccta
     3421 cggcaccatg ccgcgcttcg tgcggattgt cacgggtcgg gaagaggggg ttccggcggt
     3481 ctgcggaatc gaagaggccg tgcgccgcat gacctcgcta cccgcttgcg ccttcggcat
     3541 ccgtgatcgc ggcacgctca tcccgggcgc gttcgcggat gtgacggtgt ttgatccgga
     3601 gacgtttcgc gacaccgcca ctttcgccgt gccgcaccaa ttcgcggcgg gtgtgcgcca
     3661 ggtgatcgtc aacggtgctg tcgcgttcga caacggccgg ttcacgggtg agcgccgcgg
     3721 tcgcttcctc tcccgctgat tcaggcggac agacggttcg gaggtgtccc tgccgcgggt
     3781 cgcaggtcga cgttcacgag gacgagaggg acgcgtcgtc ggacgggttt ttttccagca
     3841 ggtcggccac ggctttccgg aacagcctct tggcttggga gagcggttgc cggatcgtgc
     3901 atccggccgc gcacgagtgg ccgccgccgc cgaactgttt ggccagcacg gtggcgtcta
     3961 gcggcctgcg ggtccggatg ctgacgcgtg tctccgcgtt gtcctcctcg ctgccgtaga
     4021 aaaagagcgc cacacggttg ttgaccaggc tacgcgggat ttcgatgacg tcctcggcat
     4081 ccgccttggt tccgccggtc tcctcgaaat cctgcccggt cagcgtcacc aacgccaccg
     4141 tgttgtcctc cccccacacc gtgagcgtac ggaaggcgcg ccgcttcagt tccatcgcga
     4201 cccgactgaa cgagcagtag agtttgtcgt tgatgaacgc ggtacggacc ccgtaccgca
     4261 gcaagtcggc accgcaccgc agggttgcgg gccgggtctg ctcatgcgca aaccgtccgg
     4321 tgtcggtgat cagcgcgacc cacagcgctt cggccgagac ggtatcaagc ggccagccgg
     4381 ccttgcgggc caaacgccag agcatctcgc cagtcgagga ggcgcgtccg tcgatccagt
     4441 tcaacgtgcc gaaaagggtg ttggtgaggt ggtggtcgat gttgatcaag ggaagccggg
     4501 atgccagcgg gtggagcgcc tccggcattc ggtcgaacgc gccgcagtca agcgcaacca
     4561 tcaggtcaaa cgaacgtttg accgcctctt cgggcgcaag cagctttttg acgccggtca
     4621 gaaagccggg gccgccgagt ccggtacgcg cggcggtggc cacggcccgg acccccgccc
     4681 ggttaagcag atgggcgagg gcgatcattc cgccgagcga atcgccgtcc ggccgcacgt
     4741 ggccggtgat cagcacgcgt ttggccgcgc gaagcgtttc cgccacttga gtgatccggg
     4801 cgttacggcg ggcgggtttc ttgtttttca tacgcatcct atccgcggac ctgccccgtt
     4861 ccggtgatcc ggtacttgta ggtggtcagc tcttccaggc ccattgggcc gcgggcgtgc
     4921 agtttgtcag tgctgatccc catctccgct cccatgccga actcaccgcc gtcggtaaac
     4981 cgggtggagg cattcacata tacggcggcg gagtccacct ctttaaggaa gcggttggcg
     5041 ctgcgtgtgt cagcggtgac gatcgcatcc gaatggtgcg agccgaaggt gttgatgtgg
     5101 gcgatggcgg ttttgagatc acggaccacg cgcaccgcga ggatcagatc aaggtactca
     5161 gccgtccaat ccgcttctgt ggccggctcg catgcgggca gcacggcccg cgtggcggga
     5221 cagccccgca gcgtcacgcc gcgccgcgcg gccagctctc cgagttgcgg cagaaacgcg
     5281 tccgcgacgt cacggtgtac gagcaaggtt tcggcggcgt tgcagacccc gggacgctgg
     5341 cacttggcgt tttcggcaat ggcgagcgcc tgggcaaggt cggccgaagc gtccacgaac
     5401 acatggcaga cccccttgta gtgtttgagc accggcacgc gggcatgctc catcacggcg
     5461 cggatcagcg attcgccgcc gcgcgggatc acgacatcga cttggtcttc cagccgcacc
     5521 agttcgcgca cagccccgcg gtcggtggtt tcgatcagtt gcacggcgtg gggcggcaac
     5581 cccgcgcgct ctccgccttc ctgcagcgca cgcgcgatgg cgaggttaga atggaaggcc
     5641 tccttaccgc cgcgcaggat cacggcattc gaggttttga tgcagagcac ggcggcgtcg
     5701 gcggtaacgt tcggtcgcgc ttcgtagatg atggcgacca cacccagcgg cacgcgccgt
     5761 ttctcgatcc gcaggccgtt ggggcggttg cgccgccaaa tcaggcggcc gaccggatcg
     5821 ggcagcagcg ccacctcgcg cacaccctga atgaacgcgt caatccgggc ccccgtgagc
     5881 gtcaggcggt cgagaagagc cgccgggatt ccggcggaac gcgcggcctc cagatcccgg
     5941 tcattcgcct cacggatctg atcgcgacgc gcctccagcg cctcggtcat ggcattcagg
     6001 atcgcattct tgcggcgggt cgagagcccc accatgcggc gtgcggcggc ctgtgcctga
     6061 tcgccgatca ggcgtagggt ttccgcaaga ttcggttcgt tgaggtttgg tgtcatatcc
     6121 gcctccgggt tacagggtgc tgctgaggat cagcgtgccg gtgtcgcagc cttccatcac
     6181 ctgagtcaac acgtccgggg tacggccgca ggcgaccacc acgctgcatc cggcgcgaac
     6241 cgcgatctgc gcggcccgca gcttggagtc cataccgcct ttggagagac cgccctcctc
     6301 ggtcggcatg accagcgcga aggcctcgtt gagattctcg atgcaggcga cccgacgatt
     6361 cttcgcgtcc aacacaccgt ctaccgtgct gaggatcacc agcaggtcgg cccgcaccag
     6421 cttgaccacc agcgcggcca gatagtcgtt gtcgcccagc gacaacccgg ctttgatttc
     6481 ctcatccgcc accgcgtcat tctcgttgat gatcgggatc acatcacggc ggatcatgtg
     6541 ctccaacgtc cgcttggcgt tggtgaagcg gatgcggtgc tggaaatccg catgcgtgag
     6601 gagaacctgg ccgatggtga tcttctcggt gtcgaacaat tcctcgtaat gcgccataag
     6661 ccgagcctgc ccgactgcgg cgcacatctg gagatccggc acgctggtgg gccggacggt
     6721 catgcccagc gcctccatcc ccgcgccgat cgcacccgag gagaccacca gcagctcgta
     6781 tcccttgcgg tgcagtctgg cgatctggcg caccagtcct ttgagcggcc gcatgtccgg
     6841 ccgtccggag ggatgagcga tcacccgggt gccgattttg accacgacac gccgggcttc
     6901 cggcaggaac tcacggtagc ggtgttccac attcataaac gaacgcaacc gggttacgcc
     6961 gcaccgagct ggtagcggac gaaccgtttg atcgtgatcg tgtcgccggt ggccttggcg
     7021 cacgcgttca ccacatcggt gacggtctgc ttcggctctt tgatgaacgg ctggtggatc
     7081 aggcagacat ccgaatagaa cttttggatc ttgcccttga ggatgttctc cacgatgttg
     7141 gcgggtttac cctgcacctg attgcggtag atatcctttt cagcctcgac cacctcagcc
     7201 ggaacctcgt ccggcgtgat ccagcgcggt gcggccgccg cgatttgcag cgtcaggtcg
     7261 tgcaccaact ccgcgaacgc cgcgctcgtt gccgtggcct cgttaccgca accgacctca
     7321 accagcacgc cgaccttgcc acccatgtgg atgtaggagg caaccttgcc tgtgccactc
     7381 aacttgtaga gcgcggtccg ccggatcttc acgttctcgc cggtctgcga gacaatggtc
     7441 atcagctcat ctttcagggc cgcgctaacg tcctcaaccc cttcgagggt cttatcggtc
     7501 acggccttga cgaactgctt gaaattgtcg gtcttggcca cgaagtcggt ttcggtgttg
     7561 acttcagcca tgccgatggt cttgccgtcc tccgaggtcc gcgccatgat gatgccttcc
     7621 ttcgactctt tgtcggcacg tttgacttga atcgcaagcc ccttctcgcg cagcgccttg
     7681 atggccgcgt cgatatcgcc gttcgcatcg gtaagggctt tcttgcaatc catcatgccc
     7741 gcgttggtac gctgccgaag gtcattgatt gccgcaactg tgatctctgc catgatatcc
     7801 tcctaaaagt gaaaagtgaa tgcgggtcgg ccgtgtcggt atcctgccgg cacggcacgg
     7861 accagcgggt tattcggcgg ccggcgcgga agcctcagcg gcgggcgcgg cctcgacaac
     7921 agcgggcgcg gcagcctcag gggcgggcgc ggcagcctcg acagcggcgg ctttggcctt
     7981 caccgtttct ttggcaacct tggcggcacg cttgcgggtc tcggcgagct tggtcttggc
     8041 cttgtcggcc ttgccctcag cgccagccgc ctccttcttg cgcgcagctt tgcgcgcggc
     8101 gcgctcctgc gcctcctggc tggccagctc cgcctctttc tgctgttgcc gttcggccgc
     8161 aatgcgagaa tactcgtcgt tggcgttctt gatcaccttc gcgaacgcgt ccgcaatcag
     8221 tttgatgccg cgcacggcgt catcgttacc ggggatgaca tactggaccg ggtccgggtc
     8281 ggcgttggta tccacaatgg ccaccaacgg aatgcccaga cgccgcgcct ctttgaccgc
     8341 attctgctcg cggcagatat cgacgatgaa gagcgcggcc ggcttgcttt ccatatccgc
     8401 cacgccgccc aagttctttt ggagcttttc cagttcgcgg cgcaggctgg aggcctcttt
     8461 cttgtggacc gagagcacgc cgttgttgtt ccgccccatg gcttcgatct ggcgcatgcg
     8521 gcggacactg cgacggatgg tctggttgtt ggtgagcgtg ccgccgagcc agcgctccgt
     8581 catatggaac tgaccgcagg cctccgccgc ctcgcgcacc gtctcctgag cctgcttctt
     8641 ggtggccacg aacaaaatct tttttccgct caggacggct tcacgcaaaa aagcggccgc
     8701 ctcgtcaatc agcgttaccg tctgggtgag atcgataatg tgaatcccgt tgcgcttatc
     8761 gaagatgtag cgcttcattt tgggattcca ccgtttggtc tggtggccga agtgcagacc
     8821 cgcatccagc aggtctttca gggtgagccc ggtcaggctc gatacaggca tgtccattgg
     8881 gtttactttc tcaactggga tcctcggcaa cgcgtcgcgc caacggccat ggaccggccg
     8941 gcacgatacc gcacccgaag cttcccgggt taatccgctt tgacggaccg gtcctgtaac
     9001 cggccgcgcc ttcgcttcca cgcaggggag ccacccggca tcccgcgcta gggaataaaa
     9061 aagaaagata ccctttttcc ggccagagcg caaggccccg gctgtttttt gattcaccgc
     9121 ctcccgaaaa accgattcgg cctgcttgac tatcatgggt gagtatggtt gactatttcc
     9181 cttttcccgt atgatttttt gaaccggatg cggggttccc ggttcccttc caacccggta
     9241 ccggtgcctc gaaaacccga ggggagattg cctgatgatc aaacgacaaa agaccccaac
     9301 tcgacatggc gcggatggca aaaagtcggc ggctccgaaa accgaggccc cgcaacagcc
     9361 ggacgcgcag cctgcggccg ctgcacagcc tcccgccgca ccagaaccag atcagtcccc
     9421 accggaggca caggaactcg cgacgctcaa agaccgctat acgcggttgc tcgccgattt
     9481 cgataacttc cgcaaacgcc aggcgcgcga taacgaggag tttgtccggc gcgcgaacga
     9541 gaatctgctc agcgacctgt tgccggtggt cgatcatctt gaactcgcgc tggcccaaac
     9601 cccggacccc gacgacccct ttgtgatagg ggtccggctg gtttacgagc agatccagtc
     9661 gctattcggt cggtacgggc tcaccccgat cgacgcgaag ggcgagccct tcgacccggc
     9721 gttccatgag gcgatctcgc aggcgagttc gttgaccgtg ccgacgcaga cggtgatcga
     9781 gcagttccgg cgcggctgga tgctctccgg acgcctcctg cgccccgcgc aggtggtcgt
     9841 ttcctccggc aaaccggagc cggagcaagt cgaagccgag tccgtgagcg tctcagactg
     9901 aatgttccgc agccaaccgt actcgaccgg aaacatgtta ccctaatccc ttttccgcta
     9961 tggctaccca acgcgattat tacgatatac tcggagtcgg caagaaagcc tctgccgagg
    10021 agatcaaaaa ggcctaccgc aaactggcca tgaaattcca tccggaccgc aatcccgaca
    10081 ataaagaggc gacggaaaag ttcaaggaag tctgcgaggc ctacgaggtg ctcagcgaca
    10141 cggacaagcg tcagcggtat gaccagttcg gacacgaggg catgaagtcc gccttcggac
    10201 ccggcgggtt cgatttcaga cgcgatttct cccacatgca ggacatcgac ctgcaggaca
    10261 ttctcggcag tttcttcggc ggcgggttcg gcgacctttt cggggggaac ggcggcggac
    10321 gacggcggtc tcggaatccc aatgcgcccc agcgcggcga tgacctgcgt ttcgatctgg
    10381 agatcgattt ggaggaggcg atcttcggat ccgaacgcga agtggatttg tcggtcaatg
    10441 atgcctgccc aacttgcaag ggacgcggca ccacctcggg gtcgggacgc gagacctgcc
    10501 gccaatgcga tggtcacggt gtggtgattg tgggcggcgg gttcatccag atgcggcaga
    10561 cctgcccggt ctgccgcggc gagggcacga tcatccgcaa cccctgcaaa ccttgcggcg
    10621 gttccggtcg cgtgaaggtg ccgcgccatc tcgcgctgcg gatccctaaa ggcgtggaga
    10681 ccggatcgcg gctgcgtctt tccggcaaag gcgaaagcgg tctgcggggc ggcccgcccg
    10741 gtgacctgta tgtggtgttg cgtgtgcgcg gacacaaact gtttcaacgg caggatgatg
    10801 acctcgcctg tacggttttc gtttcgccgg tgaccgccat actcggcggg gacatcgagg
    10861 tgccgacgcc tgatggccac gcgcgcatca agctgccggc gggcaccccc aacggcaagg
    10921 tttttcgcct ccgcggcaaa ggcatgccta atctcaacgg cggaacgggt gacctgcacg
    10981 tccgcgttga gatcgaagtc cccgtgcgcc tctcttcccg gcagaaaaag acgctcgaag
    11041 agttcgccag ccagaccacg gtagagaatt tcccggaaac gataaaacag aacaaagcca
    11101 tcgacacctt tttcgcgcgt cgcgacgccc tgcgtaaaca cacatccaca tgaccgcgga
    11161 accctgcgtg tcggaggccg gtctaccgac cgcacaacct gtaacggatc accggacccg
    11221 tcgctcatgc accgttgcct tgtcacgact gagaatctgc ttgccgacac gctgacgctc
    11281 ggcagggaag cgtcgcacca tcttcacacc gtgttgcggg tgcggccgga cgatccggtc
    11341 gtcctcttcg acgggcaggg gcgcacacgc cgtgccctcg ttcgatcggc tgaacggcac
    11401 agcgtcgccc tgaccgctgc cgagcccgcc caaacccatc cggcacctgt ctgcgcaatc
    11461 accctgttcg cctgcatcag caagggccac cggatggatt ggacggttga gaaggctgtc
    11521 gaactcggca tcgcacggct ggtgccggtg atttccgagc atacggtcgt acgtcttgct
    11581 cccggcgacc gggaagggaa ggcggaacgg tggcgacggg tggcagcgga ggcggctcgc
    11641 cagtgcgggg cggtctggat gccgaccatt gtgtcgcccc tccccttccg cgaggcccta
    11701 ccccttgtcg caaccaccct cccagtcttt acggcggcgc tcacgcccgc ggcccgcccg
    11761 ctgcgcgagg ttttcgcgtc ccatagcgaa ccgcctgtcc acgccggatg gtttgtcgga
    11821 cccgagggag atttttcgga ggccgaactg gcggagttga ccgcaacagg cgcgacgcca
    11881 gtgagcctcg gtccgttggt gctgcggagt gaaaccgcct gtctctatgg attgtgcgca
    11941 ctcaacgccg cctggctgta aaaaaacgcc ggggcgcgtt ttgcgttgag ccgtatcgca
    12001 ataccttgta ttctgcacac atgaaccaat acgtgcgctt cgacagtgtg ggcggggcca
    12061 gcggcgacat gatcctcgcc gcgcttgccg cgttgggggc ggatctggat tctatcggcc
    12121 agacgctccg ttcgtttttt ccgcaacccc tgtttctgca taccgaaccc gcggctgccg
    12181 cgggactaca cggactgcgg gtagcggtgc ggtgcgacga ttccgaagat cacgacccgc
    12241 acgtctggcc ggatgcgcac acccccgcgc acggtcctga ctccgggcac agccaccacc
    12301 acaggccgac tccccagccg ggacatccac acggacacac gcatggacac acgcacggac
    12361 acacacacaa cgcggggcac ggggcgcaca gccacacgca ccgcggcctg cgcgaaatcg
    12421 aacagcttct ggcctccgcg ccgatctccg acgccgcgcg gaccctaacc cgcgacgtgt
    12481 tccgcgctct ggccgaggcg gaagccgcga tccacggcaa aacgccggaa accgtccatt
    12541 tccacgaggt cggcgcctgg gattcggtcg ccgacatcgt cggggcctgt ctcgcgctcg
    12601 aacagctcgg aatccgcggt gtggcctgcg gccctctgcc cgcaggaacc ggcacgatcc
    12661 attgcgcgca cggtgaaatg cccaatcccg ccccggccac gctggagctg ttggccggca
    12721 tgaccgtgac gcagaccgaa gagccgttcg agttggtgac ccccaccggc gcggcgctgt
    12781 tgcgcgtctg gacgcgtacg cttgaaccgg tgccgcgtga ggcgaccgtc ctgcgcagtg
    12841 cgttcggctt cggcagccgt acgctgcaga gccgcccgaa tgtgctgcgc gccacgctgt
    12901 tggaggctgc cgccgcgcct gcccccgaac cggaggagcg gctggtgctc gaaaccaatc
    12961 tggacgactg caaccccgaa tggctgggcg cactgacgac cgaactgctg gagtgcgggg
    13021 cactggatgt ctggcagatc cccgcgacca tgaagaaagg gcgagcgggc gtgattctcg
    13081 gcgtactcgc gcgaccggcc gacgcggacg tgttgcggga acgcatcttc cgcggcacga
    13141 ccacctttgg catccgctca catgcggtgc ggcgcgaaac cttgtcgcgg cgcttcgtga
    13201 ccgcgcatac gccgtggggc gaggtaccgg tcaagttggg tgcgttgcgt ggcgagaccc
    13261 tcacggccac accggagcat gacgcttgcg aacggttggc ccgtacccac ggcgtgacgc
    13321 cgcgccaagt atgcgaggcc gcgcgccaat cagccggaga accgaaacgc gaatgaagaa
    13381 tccggaaagc gtgttcaaac agccgccgca tgtggtccaa ctgctgccgg cgcttgacga
    13441 gggcggcgtg gaacgcggca ccgtggagtt gaaccgcgaa tttgtgcggc atggcctgcg
    13501 cagcaccgtg gtttcaaatg gcggacggct tgtcgaacgc atcgaacgcg acggcggtcg
    13561 gcactgccgc ttggacctca aatccaaaaa cgtgctcagt gccccggcgc gtgcgggcac
    13621 gttgcgccgc ctgttgtccg aactccgccc cgatctggtg catatccgca gccgcgtgcc
    13681 cggatggctc ttcgcctggg cgaaccggac actcggcctg ccgcacgtct caaccgtcca
    13741 cgggctcaac agcgtcaatg cctacagtcg gatcatgacc gccggggtac gggtaatctg
    13801 tcccagttcg gctgtggcca accacatccg tgcgcactat gccacgccgg aggagcgcat
    13861 ccgcgtgatc ccgcgcggtc tggatccgca acagtttgat ccgcagcggc tggacaccgc
    13921 gttcatggcg gcgtttcgtg accaattcgg tctggcgggg cgtttcgtgg tgttgggggt
    13981 gggacggctt acgccgctca aggggtttga cgtgttgctg catgcgaccg atctggcccg
    14041 caagcggatg ccccaaatca aaaccgtgat cgtcggcggg gccgaaacag cgcgcgaagg
    14101 gtacgcgcgg gagctgtacc gccttgcgga gtcgctgggt ttagacgacc atgtggcctt
    14161 tgcaggcagc cagagccgca tggcggaaat ctacgcctgc ggccgggtat tggtttccgg
    14221 caatcatgcc aaacccgagg ccttcggccg caccatgacc gaggcgctgg ctatggagtg
    14281 tccggtgatc gcgacacgct tcggcggcgc gctcgatatc gtgcgcgaag gggtgaacgg
    14341 ctggctggtg gagcccggag atccggcggc gctggccgac cggctcgtga ccgcttcgca
    14401 aaccgagttg accgggctgc gcgaggacgc gttggagcgt ttctctctgg aggcgatggt
    14461 ccgcgccacc ctcgccgtgt atgcagaggt gctgggcgct gaatctcctt gaatccggcg
    14521 gggggcttcc cgtatcttat cgcgtatgaa catccttgtg atcggcggcg gcggccggga
    14581 gcacgccctc gcttggcgtt tggcccaaga ctcctgtcgt cccaagttgt tttgcgcgcc
    14641 cggtaatgcc ggaaccgccg cgttctgcac caacctgccg ttggctgcgg aggatctgga
    14701 cggactggtc gcctggtccc gcgagaaccg tccggatctc acagtcgtgg gcccggagat
    14761 cgcgctctgc ctcggtatca ccgacgcgct ggaagccgaa gggttgcgcg ttttcggccc
    14821 ctgccgggcc gcagcccgca tggagggaag caaatgtttc gcgaaagagg tgatgcgcgc
    14881 ggcaggtgtc cccacggccc gttccgcgtc tttccgttcg agtgcggcgg cacgggccgc
    14941 gctggatgat ttcgcgttgc cggtggtgat caaggcggat ggacttgcgg cgggcaaagg
    15001 ggtgatcatc gcgcagacgc ggcaagaggc ggaagcggct ctggtttcga tgctggagga
    15061 ggcggtcttt ggcaaagccg gcgccgaagt gttgatcgag gagtttctcg aaggggaaga
    15121 ggcctcgatc ctggcgttgg tcgatggcga acatgcggtg ctgcttccct cctcgcagga
    15181 tcacaaacgg gtgtcggatg gcgatcaggg accgaacacc gggggcatgg gggcctactc
    15241 gcccgcgccg gtggtcacgc cggagttgtt gccggtgatc cgcgacacgg tgatcctgcc
    15301 tgtggtgcgt gaattgaagc ggcgcggcat tgtttacaaa ggtgtgttgt acgcggggct
    15361 gatgatcggt ccccagggca tccgtgtgct ggagtttaac gcgcgattcg gcgatccgga
    15421 aacccaggcg gttttgccgc gtctcggcgg cgacttgatc ccggcgctgg aggcctgcat
    15481 caacggcaca ctgagcgatt cgctgctgac ggtccgtccc gaagccgccg ccacggtgat
    15541 tctggcctcg ggcggatatc ccggcgccta tcgcaaaggg ctccccgtga cggggctgga
    15601 ccaagccgcc gcactggatg acgtgctgct ctttcatgcc gggactgctt tgcgggacgg
    15661 ccagatcgtc acggcgggcg gccgcgtgct ggctgtcacc gcgctgggta ccgggttgcg
    15721 tgccgccgtg ggccgcgcct acgaggcggt cgggaaaatc cattttgagg gtttgcattt
    15781 tcgcaccgac atcgcccacc gcgcgttgga ccgggagtga cggacaacac acgatgcttg
    15841 gaacagattt ccgaatgctc taaaccaagg agacctcaga catgcccaca cctctcattg
    15901 gaatcgtgat gggaagcgat tcagactggc ctctcgtgca gaaggccgcg gacaccttga
    15961 acgccttcgg cgttgccttc gaggcgcgcg tgatctccgc gcaccgtacg ccggatctcg
    16021 cgttcgagta tgcgagcagc gcggagacgc gcgggctgaa ggtcctcatc gcggcggcgg
    16081 gtggcgcggc ccacctcggc ggcgtgctgg cggcgcacac ggcgctgccg gtagtcggca
    16141 ttccgatccc cggcggcgcg ctgaacggac tggacgcact ctacgccacg ctccagatgc
    16201 cagcaggggt tccggtggcc acggtgacgc tcggcaacgc gggtccggtc aacgcggccc
    16261 tgctcgccgt gcagatcctc ggcaccgccg acccggaact ccgtgcgaaa ttccgtgcct
    16321 acaaagaaga actgaagcga aaagttctgg aaggcgacgc caagatcaac gcggcgctgt
    16381 gacgcgtaag atttcccggc ctgttgacgg cgcactcctc acaatgcggc cagcagattg
    16441 cggatcgcgc gttcgcgcgc ctcgattgat tcgcagaggc ggaactggac acgggcgcgg
    16501 ttgaggttct gttccgggtg gcggatcttg aaatagacgc tacccgcgag ataatcctcg
    16561 aagaaacgca gacccaactc gaaggtgatc aggcggatcg cgtcgtagag gtaatagcgg
    16621 tccgtgtcgg tcaggaactc tccggcgtgc gccatgtagc ccttacagaa ggcctcgcac
    16681 aggtcgagat cgaagaccac cttgctcaga ttctgctcat cctcgccggc atggttgcag
    16741 atcgaacgca gggcgtcacc gaaatcgtaa tggatcagac cgggacccac ggtgtcgaga
    16801 tcgattatag ccgtgccttt gccggtgaag tcatcgatca tgatgttgtt gaccttgggg
    16861 tcgccatgca tgatgcgcag ggtgagttcg cccgcctcca gcgcccgctg cagcgtcgcg
    16921 gcgacccccc gccgctcctc gatgaacatc gccatgcgcc gcgcctccgt ggaggcgccg
    16981 agtcgcttct tcgccagcgg ctctgactcc agtacggcgt cgtatttggc cagatagccg
    17041 ggcgtgatat ggaaaccggg cagcggatcg gtcatgctgc gcggatcgag atccgagacc
    17101 agcccgtgga agtggcccag caccgcgccg cactcggcgg cgtgttccgc attctgggcc
    17161 tcatcgaagg ccgtggcgga ggcgatcttg gtgatcacgc gccagacctc acccgcgtca
    17221 tccacataat agtcctgccc gtcgcgcgtt ttgacgatgc gcggcatctg ccacacgcgg
    17281 tcggtggtat ccccttccgt ctccagcttc cgatgcgcgt gctcggtcag ccggtggagg
    17341 ttctgcatga tgatctgcgg ttcggggaag accagcgggt tgacgcgctg caggatcacc
    17401 tgctcttccg caaaggtatt gcggaagatg gccagaaagg tgtcgttgac gtttccgttg
    17461 ccggtgggat tgatggtcac cagccttccg gaaacatcaa acgcctgcat cagcaacgac
    17521 aattccataa catgcccccg atttccgtaa cccctgcctg cgcacgcggc gcggcagggg
    17581 gcccgtcccg ttcagaaggt ccacgccaca ccgaggatgt ggcgcaggtc tttcttgtca
    17641 cgcccttcgg agggcttgga gttatacgcc agttgcgtct tggcctcgac cgccatgcgc
    17701 gcggtgatgg cggcgcgcag ccccacatcg gcgttgacca gaaaagtgtc ggcccgctcg
    17761 aagctgggaa tgtattcgag attgtggaac cccttcacac gatcattaaa ggatttgtcc
    17821 acatggtagg ccagacggcc ggccatatag gtgcgggttt cgtccggttc ggtgtacttc
    17881 tcatacaccc agttggcgcc ggcctcggtc gagaaattga aatcctcccg ctcgatccat
    17941 tgatatccga caccgaggcc cggcgtgagc cgtatatcca gattggcgat gcggtccttc
    18001 tcataccgca ggttgccgta gccatagaac tgcttcgaga agaagtagtc gtattggccc
    18061 ttgaggaacc agttatccgc gatggtactg tcgtcgcggg tgctgttgtc ccgctgtttg
    18121 gcaaaatagt acccggcacc gaatgatatg cgatcgttct cgccccggcg cgcagcctcg
    18181 gccgtcacac tggcggtgtc gcttttggtg ttgccgcgca caagggtggc gccggcaccc
    18241 accacgccct tccagcgggg cttgtcgggg ttgatcttgg cgatgttggc gatcggcagc
    18301 gatgcgggct gcgccgttcc ggcaatcgtc acataccctt cgtcggaagc ggccactttc
    18361 tgtttctgca gagagccatc agccaaggcg agttcgatcg gctcgtcggt cgagaaggtt
    18421 ttgatgtcct ccatattcag gacgagcggt cccgccacat tagatttgaa ggtcattttc
    18481 ccgtccgcca ccttctcaac cttgcccgtc aggcggtcgc ccgacttgaa cagcacctcg
    18541 tcgccaaaaa gcggggatga caacccgata cccagcacga tagctgtcca agcccacaaa
    18601 cctgttctca tgacttctcc gtttcctttc ttctgctctc cgcaccccta tgcgcaccaa
    18661 acaccgggga cctgcaagaa tcatttgttt gcaacacctc gcatcgtatc ccaccgtccc
    18721 agacaaaaca agcggtttta tacacgctga aagagtttct ttttatgctt gttcaataaa
    18781 gcggactttc ggtataatgc tcaccgcgac ggtggttggt aatcttgtat catccaaaga
    18841 cgttagggaa acgaggaacg aatgtccggt ggaacctcac atccggatgc cgggaagcgg
    18901 tgcggtgcgg tgctcatgga gacggtgctg gcgataccgt tgttcatgat ttttctgggc
    18961 ggcgtgatgt gggtcggcga tctgctggtc acccggcagc aactggtgat cgccgaccgg
    19021 tttgtggcgt ggaaccacgg gttgcgctat ccggacaagg agaagaccga tccgggcacg
    19081 atccaccggt attttttcac cgaggcgaac ggcgcgccga gtccatatca ccagccgagt
    19141 tccagcgacg cgcggattga tgagacgttt gattggagtc atgcggccag cggccaggtc
    19201 cggttgcaga tgcggatgcc gcagtgggtg cggtcgatgt tcaatgcggg tcaggttttt
    19261 tacgatagcg gcgtgccgct ggaacaggcc agcaacctgt tcggacgcga caaggaggac
    19321 cagcgccacg tggtggtgat gcggacgcgc cgggaggcgc aggcagatta catccggaac
    19381 cggtacggcc gcgacaagtc cgcggaagtc gccgagcaat gggaggcaat cagcgatgag
    19441 gcgtggccct acgaatagtc tcgcgtgcgc ggcgctcggg ttgctcgggt tggcgctggc
    19501 tgcggcgggt gcgccgcagg cacccttgcc gcggctaccg ctcccgccgc tggccacggt
    19561 cctgcgcgag gatctgaaca gccggacttg gcgtcaggag ggcgagctga gcggtacgct
    19621 cgaaaacgcc agccgggagt tccggcaggc gttggcgggc ggcggatgga ggctggagaa
    19681 gcggattgtg gcgggccggt cgcatttgtt attgtgtagc cggaaacgga cgcgtttttt
    19741 ggtcatggtg tgggaaaaag gagtgggcgc ctgcggcttc gcctggggtg aggacgcgca
    19801 gcaaggcccg tgaaaggaaa cggtcggatg aaaaacttca ttccgttgat tctggctgtc
    19861 ttgttggggt tggcggcggt gttggcagtc ggaaggctgc tacaggcgcg caagcaagcg
    19921 gcggagaaga tgacctcggt ggttgcggtc gcgcgggaca tcgcggcagg cgacgtcctg
    19981 accgccgact cgatcatgga gaagaagatc ccggtctcgg ctcggccggc acaggcgatc
    20041 ttctggtcgc ggtcagaaat ggtaaagggc cagaaggcgc tgcggtcgat cgcccaggga
    20101 gattatgtgc tgctttcgga cgtgggcctc tcgcgcagca tggccgatat tgtgggcgaa
    20161 ggcgaatggg cggtggcgct caccctgccg cccggcggga tcgcgcgcct ggtgcaaccc
    20221 ggcgacgagg tggcggtgat cggcactttc cgaatccaga gcaaagtggt ctcggccgac
    20281 ctttcggccg cgcccgctga ggtggccaaa gaggccacgc tggtcctttt cccgcgcgtc
    20341 cgggtgctgg atgtggctac ggaccgcgcg ggcggcgatg aggcggcagg cgagttgatt
    20401 ctggcgctgc cgccgcaaca ggcccaggtg cttgtggccg cgcaacgcga ggcgcaactg
    20461 accctcgccc tgcgccgccc cggcgatacg gcggccatca accgaatgga tatcggaatg
    20521 gtggatgcgg agacattcga caagttgttg aaagggctga aacccgttga gatgccgaca
    20581 atcccggggc aaatcgatgc cgcgggatca ggtaaaccgg cgggacaata atccgaacgg
    20641 aggaaacaca tgagcacatt cagaaacaga atgagagtca taacgtccgc gatggcgttc
    20701 gcggtgttcg cgctggtgat gtcgcccgct ctggcacggg ccgcggatgc ggaaccggtg
    20761 acggtgacgg tcggcaaaat ggaggtcatt aacgtcgcct tcccgatcgt gggattccgg
    20821 atggccgatg catcggtggc caaagtggag cagcaggggc agcagcagct gcgcgtcatg
    20881 ggactgcggg ccggaacaac cgacctgcag gtggccggcg aagggaacgt ctcggcgatc
    20941 ttcacgctga ccgtgaccga caacatcaac gctgtgctcg ctgcggtcaa acgggacctc
    21001 gacaccgtgc cggaggtgga tgtggcgatc aacctcggcc gcgtgctcat caagggcgag
    21061 gtcagcaaca tcgaacactg gaactacctg cagaaggtgg tggccctgta taaggataac
    21121 gtggtcaatc tggcctcgtt ccggcccgcg cccgaggtga tgctgggact caaggcggcg
    21181 ttcgaaaaag caggcgtgaa ggtgctgggc accgaggccc ctgcggaatc cgccgaaccg
    21241 ggcagcgtga agctggcctt ctccgggaac aacatcttcg tgtccggaca ggtctacagc
    21301 cagcgcgaca aagaccgtat cctgcagatc atcgaggcgc agaactggat cacgttcaaa
    21361 cggggcggcg acgagaaaca agacgcgacc aaaccggtga acgagggggc agtgcgggct
    21421 ttggtggatc tgacgatcgt gccgaccatg atcgaaatgg aggccgtgtt cgtgggggtc
    21481 acggatgcgg aggagaagca gatcggcgtg aatctggcca aggcgggcct gctggtgctg
    21541 gattcgacct cggcgggctt ccagggtgac ctcgccacgc gttcttcagg gtgggccggc
    21601 acctacacga tcaacagcgg gttgcagggc gcgctgaagt tcttcgccgg caacggaccg
    21661 ggacgcttcc agaacgctgg acacatgacc ttcaagaacg actcgcccga atggcgcacc
    21721 taccacagtg gcggcaccct gaaagtgcgg gtcgccacac gtgacgccac ttcgctggag
    21781 gatgtggatt acggactgct tatgaaggtc aaaggcggcc tgctaaacgc caaggccgcg
    21841 gctctggatg tgaacctcga actcagctat cccgtgccgg tcggcaacga ctacgacctc
    21901 aagcgcaacc gtatcgaaac caccgtcaac tgcccgatcg gccagaccat cgtgctggcg
    21961 ggcatgaaga gcctggtcga gcagtccaac atggaagggg tgccgttcct gcgcagcgtg
    22021 ccggccatct cgtggttttt ctcggagaag aatacgcgca aggaagacag caaagtgctg
    22081 atcctgatga gcccgcgcat cgccgggatg ccgatcgaga cccccgcggt ttcggaacag
    22141 accgcgccgg cgctgcagga gtccgacaaa ccggtgcaac agcgccaact cgaaaaacag
    22201 aaacgccggt tcttcttctt ctgatccgtc atggcgacac tcaccctcat cccgtccggc
    22261 gggtccggcg aagccgaagt gtacacgctg gaacccgccg gcgtgacaac gctcgggcgt
    22321 tcgcccgaga accgcattgt ccttcatgac aaccgggtgt cgcgtgcgca tctcaccgtg
    22381 acgggaaagg gaggggtgtt ccgggtccgc gatctggaca gtacctacgg cacgttgctc
    22441 aacggcaagc gcttgacgac cgaggcgacg ttgcgcgagg gcgacagcct gcagatcggc
    22501 gggttctccc tgcgcttcgg ccagggcaca aaagagactc catccgaaac acctgcggcc
    22561 gacgcgcagc atgccgcaga gccggacatc gagatggatc ccgctctggc tgccatggcc
    22621 gcgctctaca ccgaagagat gatgacgatg aaaaggcgca tccacgagca ggtgctgttc
    22681 aaacttaacc tgccggagat cgcgaccaaa caaattcagg atgaggagat gcgcgccaag
    22741 ctggagaacg cgttggatca ggtcctgcgt gaggtacgcc atgaattgcc gcgcgacctc
    22801 ataaacaacg acctgttccg gcaagcgctg ctcgacgagc tgatcgggta cggcccgatt
    22861 accccaatgc tgcgcgaccc gactgtcgat gaggtgatgg tcaacgggcc cgcacgcatt
    22921 ttcgtggagc gcaaaggggt tttatacgag accggcatgc gttttttcga cgaccgccac
    22981 ctgatcacca tcatccagcg gatcgtggag cccctcggtc ggcatgtgga tgaggccagc
    23041 cccatggtgg acgcgcgtct gccggacggt tcgcgcgtca acgcgatcat cccgccgctg
    23101 gcgctcgacg gttcgtccgt gacggtccgc aaattcgcca agcgcaaact cacgacggat
    23161 gacctgatcg ccttcggcag catgaccccg gagatcgcgc tctttctgga ggaggcggtg
    23221 cgcgcgcgtc agaacatcgt ggtgtccggc ggcacgggat ccggcaaaac cacactgctg
    23281 aacgtgctct cccaattcat cccgcgcaac gaacggttgg ttaccattga ggattcggct
    23341 gagttaaaac tctcccaccg caatatcgtg cggctggagg cgcggcccgc caacatcgag
    23401 ggacgcggac gtgtcgccat ccgggacctc gtgatcaatg cactgcgtat gcggccggac
    23461 cggattgttg tgggcgagtg ccgcggcgcc gaggcgctcg atatgctgca agccatgaac
    23521 accggtcacg acggttccct gacgaccgcc cacgccaaca cgccgcgcga ttgcctcgcc
    23581 cgtctcgaga acatggtgat gatggcgggg ttcgagttgc cttccgccgc catccgcgag
    23641 cagatcgcct ccgccatcaa cctcatcatt cagcaaaacc gtttggcgga tggcagccgc
    23701 aagatcgtga ccatctcgga ggtgaccgga cgcgagggca acaccattct cctgcaagac
    23761 attttcgtgt tcgaacaaaa agggttcagt ccggaaggca aggtgctagg ccagatggtg
    23821 gccaccggca atattccgca gtttgtcgag gcgctcaagc aacgcggcga cctgcggctg
    23881 gatatgggcg tgttcgtgcc caaagcgtaa acggaaaacg cgcgtaagcg aggagtttcc
    23941 catgaccgat attttggtgc tggtattggt attcgcggcc acgacgtggg gcgtgtgggt
    24001 agtgaccccg ctgctggcac agatcgggct ggagcggtcg gccacccaac acatccggtc
    24061 cgacggcgtg aagcagttgg ggccgatcta ccggttcacg accccggagc gtctggcgca
    24121 ggcctgctgg tcggccgcgc tgttgggggc gggtcttgcg gcagccgcgc tgatcgcctt
    24181 taatgtgctg agtcccatgc tgatcggcgg cgcctgtctg ctggcgggtg tgatcgcctt
    24241 tcacatcccg gccaaatggg tacagcaccg tatccggacc cgccaacgcc tgtttgacgc
    24301 ccgcctgacg gatctcacgc tcgggttggc gaacggcttg cgcgccggcg cggcgctgcc
    24361 gcagagtctg gagattgttt cgcgcgatat gggggggccg atgacggaag aactgatgct
    24421 tgttctgcaa gaataccgtc tgggcatgga cctgcccgag gccctcagcc gcctgtgcga
    24481 gcggatgccc ggcgaagacc tcagtctgct ggttacggcg atccggttga ccatgcagtc
    24541 gggcggcagt ctggcggagg tgctggaccg gatcaccgac accatccgcc agcgcacgga
    24601 gttcaaggac cgtctgctga ccatgaccgc gcagggacgg ttcgaggcca tcgccatggc
    24661 ggcggcgccg ctggtggctt tcttgatcct cttttttctg gatcgcgaac tgatgcagcc
    24721 actgctccag accaagatcg gctggtgcgc gatcggtgcg gtggtgatcc tcgaaaccgt
    24781 ggggtttctc gtgatcaaca aaatcgtcac catcgatgtc tgaggagcaa ccgcatggac
    24841 gtaatcatca tccaaatgct gacgttttta tcggtcctgt ttctggtcaa cggaatccag
    24901 gccgttatcc gcaaggagaa cgagacgctg agcaacgcca agccgctggt gttccgcgtg
    24961 ttccatcatg agatcggcac gatcggccgg gcgaccggcg cttggattga ccagacgttt
    25021 ccggtgcaga cgcgccagat ccgcaacgac ctgattgcgg gcgcgatgcc gatcgaatcc
    25081 gtcgaggtgc gcgggttgca aggattcgcc ggcgccgtgc tcttcgccgt ggcgggtttg
    25141 accaccttca tcctgacgct gaactgggcg tacggtctgt tggccgggat cggcttcggt
    25201 ctgatgggtt ggtactatcc cgtggcatgg gtcagccgat gcgcacgcac gcgtaaggac
    25261 ctcatgtcga agagcatgcc gtacgcgatc gacctgatca cggtctcgat ggaggcgggt
    25321 caggatttcg gcgcggccgt gcgcaatctg gtgcatgacg gaccgaaggg gcctttgcgg
    25381 gacgagttcg gtgtgacgct ccgccagatc gaactgggca aatcgcgcgt cgaaggcctc
    25441 aaagccatgg cggaccgggt ccagcttgat gagttccggt cgctggtgac ctcggtggtt
    25501 cagagcagtg aaatgggcgc gagtatttcc gcgacgctca agattcaggc ggaggagatt
    25561 cgccggcggc gttaccagcg tgccgaacgg caggccgccc gcgcgccctc gctgatgctg
    25621 atcccgaccg ccctgttcat tttgcccgca gtcttcatca tcatcgccac gccgatcatc
    25681 atccgggcga tcgaatccgg cgtgggcgag tatttccaaa gcgggggagg ctgacagtca
    25741 tgttctgtct gaaagtcgaa aaaggggagc cggcgggcga ggtccacatc ctgaaggaag
    25801 gagagaacac gctcggacgg tcgcgctctg ccacctttcg tctcggcgcg tcggatgtgt
    25861 ccgggcagca cgcgcggatc gtggtgcggg gcggcgtggt tacggtggag aatctgagcc
    25921 agttcgggac gaaagtagac ggcaccccca taacgggaag cgtgcagcta acggaaggcc
    25981 agcgcctttc catgggggaa agcacggttt tgtgtctgtt gcggacgccg gcaggcgaac
    26041 cttccgccgg tgccgatatg tcggccgccg agccggacgt aacgcagacc aaagggaccc
    26101 tcggcgtgga agcgatactc caggcgcgcg ccgatgagat ccccacgggc gaaggcgtgc
    26161 cgccgtcccc tgaaacaggc ggtgcgaccc gcgccacccc ccctccgcct ccgccgcctg
    26221 cccaagtcgc tgccgcagcc cctaccggcg cggagttcgg tgcgcccgtg cctgcgtacc
    26281 cagactccga tctcacctcc ggcctgccgc tcgaagcctt gacggtcgat agcgagacca
    26341 tcgagggcgc cacccgcgcc atgcagacac gggcggccac accggaggag attgaacacc
    26401 tgcggatcgt ggagcagaaa cggcacaaga agcggatcac gcgcgcgctg gccgtcctga
    26461 tcccgctgat cgccctcgtg gtgatcttcc ggccccgcac accgccgccc gagccgaaga
    26521 tcgagtgggc gaaagacgag gagggcaact acctcgacct ctttgaggcc gcgcccagcg
    26581 gcggacttgc ggaaggcggg tatgacctct gttaccccgg ccccgtccga ttcgataaaa
    26641 ccgtcaccga agacggtgtc atcctttccg ggtttgtcgg gcgcgaacgg aacgtgccca
    26701 tgcggctgat cacgcaagag cagaccgatc cgaaattcgt cacaatggac cgcctcgcct
    26761 ttgtcgagga atggatccag cagcagaaag cagacaacaa gcgctggagt tttgacaagc
    26821 cttcgccgat gcccggcttc atcggaatcc gcaacggaat cccctacctc cgcgtcacgt
    26881 attaccgcga cgacgacgag ggttcctggt tcggtgtggc cagtgtgttc cgttacgggt
    26941 accggcggat cgtgatccgc gccgaagccc ctgcgtcgga acgcgtgcgg ctggaggatc
    27001 tgctctcctc caagttcatg cgcgcctcag aggccatgga gtatgcgtat tgggaagcgc
    27061 ccgaggagct gcccaaacag agcgaggccg tgttgctcaa acaggcggag gcggatctca
    27121 accgcatggc gccggcaacc tggttcgagt tgcagcgcaa cctttcggga ttgttggccc
    27181 gcaccgtatt gtccggcaac aagaccacgg aggtagaggc gttgcgtctg ctggtccggc
    27241 tgcgtgagcg actcaccctc tggttcaaca gccagcagct tgcctttgac gcggcgcgta
    27301 tgcaaaataa ccggggtcaa gcgcgggaga ccgcccggct gacccgggcg gtgttcaagg
    27361 acatggagga ccagcggtat tacacggtcc gcaaatggaa cacggagttt taacgccatg
    27421 ctgatccgac tggaacacaa cggacgcatc atacacgaga tcggttcgga cgcgctgacc
    27481 gctgaactga cgatcggccg gagtcatgcg tgcgaatggc cgatcccgaa agaagatggg
    27541 gtcgccagtt cgcgccatgc agccctcttc aaaaaaggcc gcgcggtctg ggttaaagac
    27601 ctcaaaagca ccaacggcac ctttcacaac ggcaagaaga tcgaaaaacg caaactggcg
    27661 atcggcgaca aaatcagtat cggcaattgc gtgctcagcg cggaggccga ccggagcggg
    27721 accggccgca atctctcgga ggtggtcgtg ctctccggca aaacgcgcgg ccagaaaaaa
    27781 cggctcgtgc cgccggtctt cacgatcgga tcggacccct cctcttcgct ggtctttctg
    27841 gacatgttgg tctcgcggcg ccacgccgag attctgatcc gggaggacgg cagttgctgg
    27901 atccgcgatc tgggcagcaa aaacggcacg tcggtcaacg gcatgccgtt gcgcgatgac
    27961 aaagagcggc tgctcaaaga cggggactgc atcgcctgtt cccatttcga actcgaattc
    28021 cgcgacgggg cggttaaaca ctccaacaaa cagacctggt tgcggttagg catcctgatc
    28081 gtcaccctgc tggcgggtct gggtgcctac catacgtatc aacggctcaa accatcggcc
    28141 gaggcctttg tcaaagcagc gcgccgactg gccgccaaag aagagttcga tcaggcggaa
    28201 cgcgaagtgg aacaggcggt caacgcgcgc cacgcatcgc gtcatcaggt ggcaatcgaa
    28261 gagatgcgcc ggttgatcgg tgtctggaag cggacgcgcg cgctctggca gagcgcgaag
    28321 gaggatctgg agcgcggtga gtgggtcgga gcctcgcgca ccctcggcgt gctgcaagcc
    28381 agcaaacgcg aggtctggga gtggaacgcg caggccgccg aagccaagga cgccgcggca
    28441 tacgccaaaa ccctgctgga cgctttcctg cagaccgacg cgctgctgaa gcgggaggat
    28501 ctggagttcg ctgagttgga aaacaatcag cccgtacttg accgcgtact cgacgagaaa
    28561 ccggcgacag ccgaaccggc cgcctatctg aagcgtctcc tcgatgaact gcgccgtgtg
    28621 caaacccggc aaaccgccct gctaaccgag agtcgtcaga tggaaacggc cctcaccaaa
    28681 ctgggcgagg cccaaccgtc gtatgatgaa gtggtgagcc tgctcgaaca actggcgacg
    28741 agtcaggaac ccgccttgag acgccgggcc caagtcttgg tcgtgccggt gaaggccctc
    28801 gcaaacagtt acatcaaact cggcgaggcc gcgaccaccc tgcggagcct cgcgttccag
    28861 tcggcactga agctcgacct ggctctgccg acagtgaatg cctgtgccat cgagccgcgt
    28921 gtctccctgg cgcgccagaa catcgagaag atccacgcga acctcaaact caaggggtat
    28981 cagctcgact ccctgttcac ggaggtggaa aaacggatcg gccgtaacga cgattcgctg
    29041 gaggggttcc gggtgctgcg taattcggcg atgatggaga aggtgatggc ctgcgattcc
    29101 atgaacctgc ctctgcccaa gcgcagccgt tcccagccag ccggcgagta tgaccgcctg
    29161 ctgggcatcg aggcgttcta cgcctatctc agcgcacagc ccgaaccgct tgaccccgcc
    29221 atgctggcgg acctgccctt tgtgccgctg atcaaccaga cacgcgaggc cctgcagaag
    29281 atcgacacct ttctgcagtt catggcgcag gcggacaacc agtggctgct ggacgatgcg
    29341 ctggccgtgc aagtcaagcg gatgaaagcg atcatgcaac agcgcgacga gttggtgaaa
    29401 ctcctgatta cgcgtgccgg gtccgccacc gaccgcaccg ctctgatcgc gggcggcctt
    29461 gccgcgcgcc tgatcaccgt ttccgggaag accaagatca aagacctgcc tccggaagag
    29521 tggctggcgg ccgagctgaa gcggcaacgc accgccctgc tgcgcatgaa cgaggagtac
    29581 acgctggctg ctccgacgcg gcagatcgag atccgtgccg cagtcctgaa agccggtctg
    29641 cccggtgatc cggtggtccg gcgcatgtgg gccatgcgtg atgctgcggc agccgccatg
    29701 tcgtccacac cgtaaccgct ccggctgaaa acggcgagac cacactccca caactccccc
    29761 tactggagcg cctgtttgat atagggcagg tattgccgcg tatagacgac gatggaccag
    29821 aggttcagaa taatcagcag ggcttcaatc aggtagatca gcgcctgcgg caggaaccac
    29881 gggtggaggg aggcgtagac gtagatcaca cacgcggccg ggaagcttac ggccgtgcgg
    29941 aacttcccca tccaattggc gcggcagtcg gcctggaaca tcgcgcccac ggagcggaga
    30001 aaggtcaccc actggtcccg cagaatgtag agcaccgtga agatcagcat caccagcgcg
    30061 tgttcggcct cgttcggctg gcgccccagc agccagagca gggtggggaa aaccaccaga
    30121 taaaaaacct tgtccatcag cgggtccgcc atcgcgccga accgggaaac cacctgccac
    30181 ttgcgggcca gtttgccgtc gcacagatcg gtcagggcgg caatggacag cgatgcggtt
    30241 gccgccaccg ccaaccaaac gccgcggggg gccatatacg catgcgtcac ggccaaaacc
    30301 atgaagagca ggacgaacgg cacgcggccg aaagtgatcg cggtcacgat catccgtttg
    30361 gcggaaacat ccatgggaag tatcaaacca taactcaccg ctcttgaaaa ctcaaaaccg
    30421 gcgcgtccgg cagatcgaac ccgaagtacc gttccgcgtt tcgggtacag atgtccttca
    30481 cgatttcgcc ggtccaggcg agatcgttgg ggatccggcc ctgcgtgatg tcggtaccca
    30541 acagattgca gagcacacgc cggaaatatt catgtcgcgt gtaactgagg aaagagcggc
    30601 tgtccgtcag catgcccacg aaacggctca gcaatccgag ctgggagagc gcctccatct
    30661 gacgcagcat gccgtcgcgc tgatcgttga accaccaacc gctgccgaac tgcatgcggc
    30721 cgggttcggg accgcgctgg aagttgccga gcagcgtggc gaccatctcg ttgtcgcgcg
    30781 ggttgagggt atagatcacg gtccgcggca gggcccccgc ctgttcgagc cggtcgaaca
    30841 accgtgccat cggtgacgcc acatgccagt ccccgatgct gtcgaatccc gtgtccggcc
    30901 cgagccgttt gaacatgatc gtattgttgt tgcgcatcgc gccatagtgg atctgcgcgc
    30961 tccagtcgct tttcgcatct agcgtcaggc tctcgtagag gaaggcggag cggaaagcgg
    31021 agacctcttc gggcgtcgcc ttgccgccct ggcgcacgcg atggaaaatc tcgcggattt
    31081 ccgtatcaag gtacgggtcg gcatataccg tgtcgagccc gtaatccgag aggcggcagc
    31141 cgtgcgcggc aaagacctca tgccgccgct gcagggcaca gagcagatcg tcccaggtct
    31201 tcaccggcat atccgcggcc gcagagagcc ggtcaagcca ggcgttccag aacgcgggcc
    31261 ggtccaaagc cagcgctttg tcgggccgcc aggtcggcag tacccgcacc gcgaaatttt
    31321 cggcgcggag agccgcatgg tgctccagcg aatcggcggg atcgtcggtc gtgcagacca
    31381 gccgcacgtt acttcgcacc atcaggccgc gcgcgctgaa cgcgggttcg gccagttgcc
    31441 gtccggtttc ggtccagatc gcctcggccg tggcaggggt cagcagatcg gtgataccga
    31501 aataccttgc gagttcgagt tgcgaccagt cgaagagcgg gttgcgcaac agatacggca
    31561 acgtctccgc gtacttctgg aacttctcgc gatccggggc atcccccgtg atgaaccgct
    31621 cgtccacccc gttggatcgc agcgcgcgcc acttgtaatg gtctcccccc aaccacaagc
    31681 gggtcatgtt ttcccaacgc cggtcctccg caatctccgc agggggaagg tggcagtggt
    31741 agtcaaggat cggcatggtc gcggcatagc cgtgatacag ctctcgcgcc gccgcattgt
    31801 caagcaggaa gtcgtcgtga atgaacggtt tcataacggc acgcatttaa gcacactcgg
    31861 cggtgcaaga gcgattcctt tttatttacg tcttttgtct tccagcctcc gctccggcct
    31921 gcggagtccg gcctgcggat ccggcctgtg gttatcaaca cggcgcccaa aaatcacccg
    31981 aagggttata aacaggtgat aataagcctg ttgataagat ataaatctct attgttaagc
    32041 aagttgactt tatcaaattg tgtagatatc ttgtgcttgc cgatccagcc gggtttggcg
    32101 catactaacg gtacgttgtt atcaacaggt tttgtttagg cacatggcac agctttcgat
    32161 aactttttgc cggaaattgg cgcgggcgcg gcgcgaacgg ggcatgacgc agagcgcgtt
    32221 ggcgcaggcg gtctcctgtg cgcaatccgc gatcagcatg ctcgaatcgg gccagcccgg
    32281 aaaactctcg caggagacgg tcggcaagat cgcctctctg ctgggcgtgc cgctggagga
    32341 ctccgcaccg gacggcgtgc cgctgccgac acgcccgagt gcaggttact gtcccaacgc
    32401 ggcctgtttc tcgaacatcc cgtatgtggt ggcggatgag gtgctcttct ggccgcgtcc
    32461 gcagccgacg gcggcacggg ggccttactg cgtttactgc ggggaggtgc tggaaccgca
    32521 gtgtccgcat tgcggcgcgg gtgtgacgcc aggggcctgt tgcccgcaat gcggccgcgc
    32581 gcgcatcacc ggtggcgcgc cggacggcgt tgaccctgaa gattgggcgg cgcggcggcg
    32641 gcgtgaactg gccgactggc gcgcgttaag cgcttggacc ggattcgctt cggaatgacg
    32701 gggataggac ggaacggaag aaagagagga tcagagaccg catgactaag aagctcttga
    32761 tcgtcacgga cgggaaggcc ggccacgaga accagtccaa agccttttgt gcggcattgg
    32821 gatacggcta cgagtgcgtc cgcgccgttt acccctcccg tttccacaag ggactctcct
    32881 atctcatcga ctggctgggc ctgctgctca gttccccttt cacgatcgaa ccgaccgacg
    32941 gttatttcgc agccgtggtt tgcaccggtt cgacggcctt ctatcccggc aaaatcgagg
    33001 cgcggcggcg cggcatcccg gtggccgcca tcctctatcc gtccggatac cggaaggcga
    33061 acttcgactg catcctggcc ccctccttcg accggccccg tccggccccg aacgtcatcc
    33121 cgatcccggt caacctgacc tcgacggacg ccgcgtttta cgagacgggc gtggcggcgt
    33181 tccgcgcgcg gcacacgcag cgcaaaccgg cctccgccct gatcgtgggt ggccccaacg
    33241 ccgcggccga actttcggtc gagaccctgc gccgcgatct ggaccggttc ttcgccgtca
    33301 cggaagggaa cgaacgctgg gtcaccactt cgcgccgcac cccgcccgcg attgaggagt
    33361 tcatcgcctc ccgcccgttt gactactcgc tgctctacag ccgcgacacc ttcaacccca
    33421 tccccgcgtt cgtttccctc tgtgaacacc ttttcgtgac cgccgactcc accgggatga
    33481 tcagcgaggc ggtcacccgg ggccgcgccc gcgttgaaat cctgatgaca ctgcgcaaac
    33541 cggattccaa gttcggccgc ttcatccgcg atctggaaca ggccggagcg gttcatgtgt
    33601 ttgacggcac gcccggttgc gcggaccgca agatcgatct cgcaccggct tttgaacagg
    33661 cgcgcaagct gctcgggctc tgaccccgtg ccgattacga atccggccac aaacagcatt
    33721 gcactttttg cccctgcttt tcctgaagat tccgttttag ggtttcaggg tttcatccaa
    33781 atcgggatcg gggtcgggat cgctatcggg cttgggtttc agggtttctg gtttctggtt
    33841 tcatgtgttt catggcttca tccaaatcgg gatcgctatc gggatcggga tcgggatcga
    33901 ttgtttacca cgaactcacg ggatggcact gctcgggttt ttttggaacg ccagggggaa
    33961 ttcctgattt tcatgaagtt tcatgtgttt catggcttca tccaaatcgg aatcgctatc
    34021 ggaatcgcta tcgggatcgg aatcggaatc gggatcggaa tcgattgtag ggtagaaaaa
    34081 agagcagttg cgttcaactg ctctttctcg gttc
//
LOCUS       DAOSLE010000014        34035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1470_length_34035_cov_6.864244, whole genome shotgun sequence.
ACCESSION   DAOSLE010000014 DAOSLE010000000
VERSION     DAOSLE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 34035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34035
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_34035_cov_6.864244"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..3359
                     /locus_tag="PK576_02150"
     CDS             327..3359
                     /locus_tag="PK576_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE60423.1"
                     /translation="MRTSVIGTVMVVCAICVQGATKFWTGEGADDKFSTDGNWEGMAA
                     PVNGDTLVFQGENAVAENDFDSTVQFAAIYMSNSVPFTIRGNTLYLSGNRIFAKSLQS
                     EITDRFEADVVFSGGASLGAASVKQHHITFLGTVASASGGTLSTDSQTGNIFTFEGMV
                     TNFSHVYRPNGNGTLRFFAPTNHFTNPSSFQINQGTITVSNLTAFGTDTRTFSGGQGR
                     YNTPAIIQMYPTADETAPIHFKVYSPNQQQSNLQLYNNTPGVTLTFTGDVSLGWTVGN
                     GGRIAVGGAGDGMITGDFTSPNLALYKSDTGIWELAGAYASTGVVTVAAGTLVVNTVL
                     PTNTLCSVTAGLLCGTGTLHRAILSSTSQITGGVPGMPGTLHFANGGEVALTVASGTL
                     VARPKADGTPSLVAVDGTVALTGLLTVNLDGDFSALPAGTYPLLTWTEKTGTGTYAAG
                     SGFPSGSSLVETAAGLALYVPGAVLSWTGAVNGDWDTATANWNSGQVFADGTDVVFGD
                     DVVNGQTNVTLTTAAAPGSVTISSDVNGYTFSGAKMTGTMTLLKTGNASLTLENDNDY
                     TGLTTINGGTFRLNGSLDGSPLLVQPGAELMQGTNASIRGDVNVNLLGQATLSGSNTF
                     TGDLVFGSIAEGKATGTVCNVSAIGGDGGNLVVMHNARVMLDAEGVYPNRTLRFAARQ
                     SGAGTVPYLKQMSGKTATWPGGIAFDAPAYDYSFEISGTLNLGTDPDAVITDAGSASA
                     STLMVRGTGRLNQYARAQLKGGFGKTDNVSWHVYASNNVLSQFNISVGSAFLHAPNAL
                     STNCTIKLGQVYGNNKFNSLFDFGGFDLAIAGLAVDQGISIEGNTQVFTTSEPATITL
                     LGPGDTILNRTGTELKGALAIRKRGAATTLFLGQPNSNTGDFRLEEGYTVASTNRAFG
                     YSTNLFVGATGTLTVSASDALPPEDDVWLRVADGGTVVLSNDVAATIFGLEINGRPRG
                     AGTYGGEGSGARIILPAVFSGTGTLSVRNGAGTILRLH"
     gene            complement(3351..3473)
                     /locus_tag="PK576_02155"
     CDS             complement(3351..3473)
                     /locus_tag="PK576_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60424.1"
                     /translation="MIRITAKMSTEDVGKVIDELMDGAVAKAQRALIGAPAALV"
     gene            complement(3536..4051)
                     /locus_tag="PK576_02160"
     CDS             complement(3536..4051)
                     /locus_tag="PK576_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOE60425.1"
                     /translation="MSGLWQKIRDGFVRTLEILLMGMVAVMTLTVLWGVFTRFCLGHQ
                     AHYTDELARVLLVWISMIGSALAFGAKAHLGVDYFVSKLHPEARKTMSVVVQLVILAL
                     ALIVFVIGGWGLAMGQMGQQLPALPIKRGMVYISMPIAGVLIILFALENMVEIIRTPA
                     GKLGAQTKSEG"
     gene            complement(4107..4346)
                     /locus_tag="PK576_02165"
     CDS             complement(4107..4346)
                     /locus_tag="PK576_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOE60426.1"
                     /translation="MLLQGLILMVVGVGTVSGFLGLLVVILTLSSKIIPRFNHILPDE
                     QAKVKAQKAQKRDASPDAEIAVAIAAAMLRQRAHE"
     gene            complement(4366..4704)
                     /locus_tag="PK576_02170"
     CDS             complement(4366..4704)
                     /locus_tag="PK576_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60427.1"
                     /translation="MQNQPIPEHSNEAGCLIRLYWMFLGNAILLILLAYLSQNRPRFP
                     SLPDVAYLLVLVSLVAARYIDIRRFNGANGENSAPATMADWRRYTLFLVTGCAVVWLI
                     IRVLVPALAK"
     gene            complement(5036..6526)
                     /locus_tag="PK576_02175"
     CDS             complement(5036..6526)
                     /locus_tag="PK576_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOE60428.1"
                     /translation="MKKNWGMTLLRMLIGWHFLYEGVWKLIQPGGWSCEGYLRMSQWF
                     AAPLFRHIANTPWLLRLCDLANMWGLTLIGLALILGVLVRPAAVAGMLLLAFYYIAQP
                     PFLAYTSEGHFLWIDRNVVEAAALLAVCLVPSHQIGPILWNRLCLFLRRRDIAADGGS
                     GLGVSSAGRRELLLSLSSLPVIGAFGYAFSRKHGLAWERENLKTTDAVTSATVKSFSF
                     DDIKDLKKPVDKFGQIGNLKLSRMLLGGNLIGGWAHARDLLYTDKLVKAYHTDERVFR
                     TFRMAEACGVNTISTNPALMRVINDYWRKEGGKIQFISDSGHKDGLVRGAELSVENGA
                     SAVYCHGGRSDNLALKGQFDKIGEAVEGMRKLGVPVGIGAHRLETVKGCVENGIIPDF
                     WVKTLHRTEYWSARPEAGYYSRDNVWCLDPDDVIAYMEKLEQPWIAFKILAAGALHPK
                     DAFPYAFQSGADFICVGMYDFQLVEDVNLVNTVLDNPIKRSRPWRA"
     gene            complement(6651..8915)
                     /locus_tag="PK576_02180"
     CDS             complement(6651..8915)
                     /locus_tag="PK576_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60429.1"
                     /translation="MKDRLYLTLGAIVAFSACAEEMPKTPFDGTTPVTVETKTFRLII
                     NPDATAKSLIVKATGEEMLEPREGIPVFASTQIRPFNNEIRLVQQAKRTTYPANRVRR
                     EGDLIFIGFETAPYQIAVRVTETAAGYATFRPERLISNTTDEHQYYHWNLDVPPIDAF
                     RLLQLPVKNRANFGDWLNVMWDDQAFVGVMGGTPYMDVDNERRHGFRKLTVESLKDYG
                     VTNGVAVLAVESQKERFLDQIDAVERDLGLPRGVQSRRDSRLNASLYWTAGDFRPENV
                     DALLSYLKKGGFRMLLVYFPGIVKAKSYNTLPDYSFDNGWDAEKLTAALKRFHDEGIS
                     VGLHTLQTFIGFKSSYVTPEADHRLALVKRFTLAKPLPLSEEPCEVFVEENPVAAPMH
                     PKIRILKFGTELMTYTGYTTTRPYTFTGVKRRHLGTYAKAHPLGEIGGVLGVSECAAI
                     SAYVDQDSSLQDEVAEKIAGVYACGFDFLYFDGSEDANPPCTVNISLSQYRCVNACTA
                     RAGRAPLFTQGCAKSHFGWHFQSGANAFDVFGPEIFKKKIIEYPYAAAQRLAKDFTRV
                     DFGWWGCWPARLDGPPPAKEGLFRSGSRTVGTQPDIWEYGTSKAAAFDCPATMQLSLD
                     TLKKHRRTDDLLETIRRWEDVRARKWLTPGQKELLKDTTKEFHLYRNSRNEYELVEWR
                     QLDVAGDKWSRVRAFVFERQGRRVVAYWDIADRSELVLANGVGTLKAENLIYWETQMN
                     EADVHAAFAGATIR"
     gene            complement(8936..10885)
                     /locus_tag="PK576_02185"
     CDS             complement(8936..10885)
                     /locus_tag="PK576_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60430.1"
                     /translation="MLERRGGCHWFWDIDVVPHKEDIDLTGLDVNEEARFEYRGLRYF
                     AHRGLTRFQAEHWGLDDWKREIDWCLKRRLNVFMLRIGQDDLFQRTFPDIVSYPDASK
                     NLPGTGKGYDNRTLFWSLEYRGKLRHDLQQYAFARGLMVPEDFGTMTHWYSRTPEEFL
                     EKMNPPFLPQTTAGYSEKNGLVWDVRDDKWVDAYWKLTQTAVEAYGQGKDRQQLLHTI
                     GLGERRCYTNRQENLELKIASLKKFLARAHRDYPNAKTLLAGWDFYFTWRPEEVQELV
                     RSLDPKRDIIWDYEGDSTRDYRPEMLDLGGNNFTKWGVVGKFPYTFSVFLAFENALDV
                     RANYPLIEERQKIVQDDSACVGYIFWPESSHTDTLLLRFFTANAWSSTSVSHRQVLDE
                     FCNSRYGALAPRMKSLWEKVIPVSYMSDWGSNYSYLFTRKSFGTKQYGPAGKTDAETW
                     GRRLAEAKGVFAELAKIEWQGEAVRRDTIDLARTVLDRMMVMEAGTLAADFEKWQGGA
                     ENGAKMIAAANRLAGLADRMADLLELHTDYSLWESYLRLNEIEPVRNPNFPSVLFENA
                     ANTYCQSHQYELARYWYAPRAHEWAAKIETIIRSKDRKSPLQLESGEVLRNELQKRPL
                     ETLRPRSDRSPKVFAALMEVLSESP"
     gene            complement(11209..14946)
                     /locus_tag="PK576_02190"
     CDS             complement(11209..14946)
                     /locus_tag="PK576_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60431.1"
                     /translation="MKLSQTRGKGRSAGAVRGGMAMFAVAFAQMAVAATDYVWTGSSA
                     SGPVSMPDWADEDNWNPKSIPNSDDALVSVSGGWVSNTMPVTVARVENAGSTTRYISD
                     HQFSIVSNLFSKNMAFFAPLHIKGSTPYLRNCSLAGDMTFGNGAKILVHGGTFSINDN
                     QYAPSSETTWRPSPLGTITEYHGSLSFNGPRGSSAAITQKFSTQAGSPFVKAVTAGAA
                     DTLPIGALVSGAGVSGGTFLRRIFGKDDFVELSQPVSETAAAAELSFAGRKGDCRRYV
                     NELAHNGSTDHNFTINLGKYRMEDIYEVEVGLLKLLTLSGGNRWTRIHCNNTFPGRLI
                     LHDTPKVLNTALEVGTCDILFSDAPNTENGPGLPYATVRQGDAGQNSRFSVTGNMEAV
                     IHEFQAIKGSLTKGGAGLLRVGFGEGPHTGALVIEEGTFVPTAEDESCPIEIGSFSIT
                     SNAVLQLPVQGLRVTNRVGSLAGGVVKGPGKLFFSNVASIDGLTLEDGAEIVFECDGS
                     IIRSEGGETVPGNPAFWVDCSKNGSAMTLVDVTNGVKGVTRIEDVRQKNDPDGDYIYS
                     EVCSAPGSGYFPELRTDTTRNIQYVHFEGNFVSGSSSPADSDTHRWSKPVKNIRHVFQ
                     VMAQDRWRSGGNFLGIIDTQQTGTEAEAWAHYNVYTGKIFDWGTDAIKQGKLWINGVL
                     SNPDQGYPYPAYENASVKYFVPIVLAFECNANYELPGADSYEYNTVRAKEGRIGHKCL
                     GELIVYTNALTVVECKQIMAYLTRKWQTTEICYAPIQTGSEASLGTLNLGEGSGGIGV
                     KDGAELMVDRVTGAGTLAKTGNGTLLVPALPEDGSVSFLVKEGTLEIQSVAATENMLP
                     AGAWARYDASNLNNLEYTVENGVTNILKWLDSASGEHPASHPDYCTNYPTLRTSTALG
                     GMPYVDFGEAKRWEKGGLPTNQYAPSAPVLSYASTPQLHTVIAVWGADHGGGNLVGTS
                     APDSQYWSSVYTIVRAGETSGPLGGDRDQPVVRSDMLMPKTAMDERGVRFHKNGVACD
                     PRQTGLSAGFNIVSWASYENFGGSALTGVASSTRYAGGEQFAEYMLYEKQLSTREVAL
                     VEAYLRQKWFGQPTPGWNGTKASKVAVSNGATLKVVGNAPLSVETLSLAGGTISGGVA
                     LQSAADLEAAVNADGTVTPVTVDGTIDFSKGGTVTFTGATNMLTYGSYVLLTGIGECD
                     LSNWSVDTAGLPGRYQNIVLSAAGGSLTVKIVPGGTSIRMK"
     gene            complement(15027..15605)
                     /locus_tag="PK576_02195"
     CDS             complement(15027..15605)
                     /locus_tag="PK576_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60432.1"
                     /translation="MYDVIAPDAKSQKPEELLAVMKSYKALVVAGDYPDTSWEKTLAR
                     YEADGGKVIHVGVDMLADPVKIGKGRGIGNGETRYPKLEKALNDLEAEYFPFEVEGDC
                     MYGLTVADRHSWLYVFNNDGVTKFADAPQELDATKTSDIRITFKSDKKRLCAVRELLS
                     EAVVAVSAGVSFDCRLGPGEFAIFEITSAGLK"
     gene            complement(15667..17076)
                     /locus_tag="PK576_02200"
     CDS             complement(15667..17076)
                     /locus_tag="PK576_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60433.1"
                     /translation="MKHAQVRYVFPRGLLAAGLGLVVLTGCDERDVCGVPLVEVPARH
                     KPLEIMGRDCWRVLTRYTPYGWSGKLLAADNPEFNNSLAISDVVPKYVAAAEPPAAAT
                     GYAVWPTLDLHFRPDAPDREILTSNPHPDRPFFLSKRGERNRGTWGSPVQLDKAAYAA
                     WRAAHPNLVADGACSEWCNDLNNAWSSVAGEKGTYVDHRPDSSNRVAILRAFLGEKPK
                     NRYEHVALLKRYYEMRKERNYGGTMSVLDAHLNSLHIAADFGAVLLRMETTSSGQYRY
                     QPSAMFTRGAARQFGIPWEWYIAGYANGPSAKGRGFLGDAMCRYPATPEATGEPRYAK
                     EREASWCPGGKIRIGNSGPDFGISRSLFKRTHYLAYLSGANYIELEEWNCGILNMWDK
                     EKGRTVFSPRGEIYAAFAEFIKDHPDRGTHYSPVAICVPLAQGYPTWGGGAVRRSLVR
                     LHRRRQGGRCRLLYARTRL"
     gene            complement(17498..19300)
                     /locus_tag="PK576_02205"
     CDS             complement(17498..19300)
                     /locus_tag="PK576_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60434.1"
                     /translation="MCTCGADKYLWTELIAFDNTEPDYGVQAYLDKMSVKPKGVSFLI
                     FDPELFHAHTDLSSDFQIGDLHCSYFARPWNEERLRQKWTAWQFRGLIAELKKYGIAS
                     YPSFFDMAPGKGNSWLRQFKATRKDVTWFDRHPEVGYRMKDGRRANNVNPIERLADGR
                     DYADFFVAQAVQFLTDYGFAGLHACDGFGHPRYPLNQLDYSHLFPEAKDRASLCRAHA
                     ARHAAFIAKLAAALHARGLKLYANTSWTRDPLEALYRHGIDYRLMEKAGLDGFMAEAS
                     AIVLELEGWRFSPVPLLDSCRVAFLLTGATVDTPIIHLACVKDGMEQYNSLRDAPQRV
                     TAEVVLLGNLLRGDRLMVPDVLWCLTDGFTREEWRRLDTAWNLAKCPKRADGVRVVVS
                     SHAMDAEFDAYCSGGYPSAYTLLAHLVNAGAAVNSAVTVEEAKADADLPLLILNSGRF
                     PADELASLTNRSAAVVTFGYGSPACPFGPRPSVLDAASWRHPLPFRAFDPQALAAAAE
                     KVNRASPVFPGADMADLRLSSYVAEDGSRIVFAVNDRPTYLNANILVRGPVTTAEALT
                     ESPSLPIVISAKAEDLTSLVAKIPPAGIVVLRLK"
     gene            complement(19353..20750)
                     /locus_tag="PK576_02210"
     CDS             complement(19353..20750)
                     /locus_tag="PK576_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOE60435.1"
                     /translation="MKILVGSFQCESNTFSPPKAGKENFYILHGEEAKNKLFASHLFE
                     EAGFEVVPLVYAVSLPSGMVTRECFDEVLADFRKEAAKHSDAAGIYLYLHGAMCVEGL
                     GSGEEFTVKELRKTIGDQIPISLAQDFHGNLADGLIPNVQAISGYRTAPHTDYDETET
                     RAAVALMRILNEKAVTVPHFFRIKVQFADAAQTSVEPYVSIMKMVRELDTDPDIISAS
                     VFNGQPWVDAPCMSASVIVYSKANHEAVHAKAKAIADYFDAHKTDLKFGVPALAPDRT
                     VDAVKTMAKPVFVSDSGDNTTAGAGGESVFLLKRFLGSDVKTLFTSLYCPTAWAKLTE
                     MKPGDRATLTIPKSDEYNDDLMIEGEYIHGAKILGFVGEESGEGILFRAGHVDIVFAS
                     VRTSFTMKEHFAAMDVAAADYDAVVVKQGYLWPGAAELAASQIFCMTPGTATNDFATL
                     PFKNLEGKYYYVRPQ"
     gene            complement(21040..22212)
                     /locus_tag="PK576_02215"
     CDS             complement(21040..22212)
                     /locus_tag="PK576_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOE60436.1"
                     /translation="MSYYNQNETNKRIAAVQAILDKKNLDAALVYFDELNVANGWYLT
                     GWCGQFEKGSVFVPAKGEPWLLGGPESEPFAIQSSAIKKIRCFPVFMVPGEEYPNAHI
                     IGFEDLYKEMSAAGMPLKRIGLVGTNTIPHQVYLDFQKGFAGVELVDITADYEALRAF
                     KSEWERENMLKAVGLCDLAYDAMKAKIKPGVFEYEVAAAGEAVCRANGATNFAYSTIV
                     GSGVRANAVVPTATNKEMKAGELVMIGIAPRFNGYAGTFGDTLPVSGVFTQDQKECMN
                     HLREVMWLTKDMLKPGVSGKEIDVPGREYFKKHGLFKYLVCPFAHTIGLMEAEAPFYG
                     PNSEDVLAPGMTVMVDVSFFGHPKFNGARIETGYIITENGSKPMSPKMDAYFSRSL"
     gene            complement(22223..22597)
                     /locus_tag="PK576_02220"
     CDS             complement(22223..22597)
                     /locus_tag="PK576_02220"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOE60437.1"
                     /translation="MSVISMEIGRKQWVFADGDLPPRGDSEPFGHEALMIVNNGEQDA
                     TIKVTILFEDKEPKEDITLHVPARRVKCFRMDYPLGDEQYQLGPGQTATILASDVPVV
                     AVYGRLDRRKDMAYYPVQGFSC"
     gene            complement(22691..25927)
                     /locus_tag="PK576_02225"
     CDS             complement(22691..25927)
                     /locus_tag="PK576_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60438.1"
                     /translation="MTVRKIGTTLIAMIIGSVLADEMLENELVSLTFDGQGCLTSIRE
                     RQSGRELVREKSPFVSVVNAEGKTLVPTSCAKDAKGNLCFGFAEAGEMVLAVRPFSGG
                     WSLGVERQTVQDAQEIHIGRVHPDCLKYRGHMVNAYSDERSFVCVRAYDVGASFRCAE
                     GLVEASVKTSARPLTGARFGFAAGPRSAAIPALRAMTIDADVPCSDRGGAWALGAEAC
                     RRSYWMAWGIGPFTIDDEIEQCRRGGFGTLHFDHFFKSYGSYELPGDGSGRDGMTLLK
                     EMIAKAHDAGLHCDFHSLCACVARNDPIATPECQDGLMVTAAYTLGTDLEAATNVTTL
                     VVNEKPVDCHEFETTAWGKSNTLKIGTELVTYTGRSTEAPWTFTGVTRGAFGTTVKPH
                     AKGTKVEYLFNCFSCFQIDADSELAKRAWELQGKLFGAGFDNYYLDGADCIRDKRKTD
                     KFVRELYKAAQANGKTPLFEDSLWTSAAWWFHSRIGANDYVQWGSKRSLDRRFERLVS
                     QARDANFIEPEMGWWATLQGNGAQGNPYRIDEHEYFGRKCAAYDAAMSVLPGNAYPAR
                     YMVARNIHRYVTVIGWYERFRLAYAFNDETLAKFRVKRDEYRLRQDTDGVWKVRPLDF
                     FHHRVTGPDARAWRIDSKERREATLRIEALLTCKPWDDPEAKVLLDPACAATFEKSAA
                     KGVTVECANSQDSERGHVVTITANNANATSRGAWSCVARVWPKPPVGQPPVYGDCSGS
                     RGFGLWVRGDGSGALLNVQLMGAKGAGSGPKSEHYQRLDFTGWRYLEFNRRERDSGAA
                     SDFVWPYVEKNSSIDYSLERVNVSKLQAVNLYLNEIPAGGKTEVSVSAVKLLPERKQV
                     TYSGLAVTLNGTRYACPFALEPTDFAEMEDGCWTRYDASANPLERAPADLAELRAGTN
                     ELTFEGNGGEGVNVRAEVNILALGEPAAALKPTVNAAAHEALAYEVVEPATWAPSKGF
                     EKIPDLVTRPGEKAALSFEVYGPTAPFTLVVGDEARRFDCTLSPKEYLVCRDGEHWQL
                     KRRGEKPCIVASGKIRPFAPFTGSRKVAFSSDDPEQANVFLIFAKHYR"
     gene            complement(26007..26786)
                     /locus_tag="PK576_02230"
     CDS             complement(26007..26786)
                     /locus_tag="PK576_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE60439.1"
                     /translation="MKRAIVFNIEKFATHDGPGIRTVVFLKGCPLRCMWCHSPESWSF
                     EVERYPDGEVIGREMSSEDVLAEVLKDKDFYDVSDGGLTVSGGEPLARPAFLAELLGK
                     AKAAGLHTAVETSGAATEKVFEPLLPLVDLWLWDVKKLDADKHFTYTKRPLAQILANL
                     RRVNDALAAESPRKRSIVLRCPMIPGINDGAEDIEAVGRLADSLPAVIELHVAPYIPY
                     GIDKARRLGLKVYEAPQPPPEYGRRIVAALTRHTAKPVRLP"
     gene            complement(26798..29179)
                     /locus_tag="PK576_02235"
     CDS             complement(26798..29179)
                     /locus_tag="PK576_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE60440.1"
                     /translation="MKVKRREFMGSAAAFFAALGLDAPAALSAARERPFAAADYETFR
                     KSLRAFYPDECHGKCRDPRQVASVRMIGNELDAYVAAHPDCDVLDIRRESYLAMRRHF
                     VPYILPGSPFYCEAGVNGGWCVWQGAESVPGRHINRICRGWLEERYIPREAFARLRAR
                     RRGCLMLTGDFVDDMHHIPPFHTIFTKGFRGVRREVETALASCPAEDASGRKGLEVAL
                     VGLDTVHEIQLAFVREAERQLAAVGLSAEDRLRLERIAVAARRCPWEPPRDFYEGLNT
                     LWFVREILGYVEGTLNFSLGRPDAWLIDFYRRDLAAGRLTEPEARDLIARFLVMADCH
                     LDTRLEIDGYSDQEAEIPLTLGGCDAEGRPVYNELTAAFLDAHIGLDLVFPKLHCRIS
                     EKSPQEYLEKIGAMLMRGHAVFAVFNDDRHIPNFMALGYPVARARDYVCCGCWDGNVD
                     SDTNLDTANYMSVARILELTIYRDPNFEREARITIDPVDDATSFEQVRDIVYHNFIRF
                     LRDVLADYTRYGGENVHVSARPVYSSCLTGCVAARRDALAGGAAFSPRIITLAFLGNV
                     VDSLCAIRKICFEDRMATLPDFLAAVRSNWKCPGGEALRQAVLAAPYWGDNSEASNGL
                     MRYWIDSVSEDIRDLKNEKGGPYVLACWIYREFLLWGLKMRATPDGRYDGDRLAQGFA
                     PSEYRCKAGVTTVFNAIGSLDHSRLYASNANLAFEKSAMNAELFAAVFRVACRKGMHL
                     LQPNCYSVEDLLDAQVHPERHQNIMIKVCGFSARFVALSKRWQDEVIQRHRLH"
     gene            complement(29307..32483)
                     /locus_tag="PK576_02240"
     CDS             complement(29307..32483)
                     /locus_tag="PK576_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60441.1"
                     /translation="MQLNMLGKWICALSVAVTFSVCGSTWTGGGAAGDWNDSSNWDGG
                     VPVADSLADFTGDVVISSAFSLPGKVTIRVAANKRVDFNGVISGTGPLVVGEKGSASN
                     YGSVYFNATNAFTGNLEVLHGFVYGTVDNAFGAADAGRIMVNPDQTAVSRTQLYYKGI
                     RSLRTLEFTLVDQKTVTFDGVTTVGTIVKKGNFRGTINGKLFVTGASNLDSLLLFSFA
                     EGAELVFTNNPSVLLPYVYTHKGKVILASVSNTVNRCTSGPYAPLGYPWSRIAFTLRC
                     DVNGAITESSALCFEATDNTFYPGYSKLDLNGTTQSVRRLTTYTDPSWDKWNDVTRLG
                     RVTSSVGGLITVNDGVDFINRNVFEGTASLEMKLASGITGTLLAKSTSSGELIATSGT
                     TVLTNGAQWVGTVRVGNGAVLKTCSADAFSNGVARIFLEGGTLELAEGSYSVKELRDA
                     TGTSLAFGWYAATASGEIKACEGLSGAGVIFVAPPDDPPEQAWTWCGGGVDDKVTTLG
                     NWKNATELPDFGYTGNVFSFPSNATVELDVPVNAKSLVFDEDASGTVEFTSTPEANGR
                     LMLANGVIDIRPGTSDRVKKIVFKSPFKFGGDLAASVPKGSSLSFESSFDSAGGASFE
                     KTGYGELRITGNANKIYGDVTVRTGHTYLTGLDPLGGTGALYIYPEAEKHKETALYLS
                     NAVVNRPVSRINPDGHNQLYALASTTNVLAGLVSLQNGFFTFTVQSKARLDFAGDFML
                     ITQRLLYPSLVDDASEVRFTSPHLNDNDAVLYGASSELNNYARFVLGASGSKFATDYN
                     FKICCTHVYSAPNVFSNAVPIQIVSPKARVDLGGWRQAVANCCCEGVLTDGVLTACGG
                     DVSAANGGTIFGASGSKLVLTDAIPCQGTSNADTGIMPVFGGAASVERAGAGTNTFRR
                     ANISTGEVSVVAGRLVFAAAGDQWDFWQKVNNVWTRYPQTSATAGTWAGKKATVSGGE
                     LVIGHSYAFPKDAEFYLEGGTLELASGVVQQIGSLWLKKNGDWVKCKDGFYGSATNLS
                     VPPENRLALITGDGTLLAGRVGLAIILK"
     gene            33012..>34035
                     /locus_tag="PK576_02245"
     CDS             33012..>34035
                     /locus_tag="PK576_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE60442.1"
                     /translation="MTDSKAKRVGVLVELTREFGRDLCKGITDFACSHEGFIPFFITP
                     ETLKRKSELKSYDGFIARVMSDEMAATLAALKKPIVDMYYDKPRPGFAIVKTNHSRIG
                     RLAAEHFLERKFKNFAFCGFAGGRFSNYCSRAFQRALAAKGHRCHSYQPDARAKYEFD
                     STVLINEQLNRAPDAKALAKWIGLLPKPVAVFCPNDLRAWQLLQVCKECDIHVPHDIA
                     ILGLDNDVIVCGFSNPMISSIDPDTPAIGREAARTLVEMMNDPDFRKKTLIRQVNPNR
                     VIGRASTEVYPIDPPWLSDALVYMRKRVSDQLTADDVFKHLGLSHTTVNRTFRKVLNT
                     TVQKEIA"
BASE COUNT         7280 a        10646 c         9333 g         6776 t
ORIGIN      
        1 ggcggagccc gcaatctcaa aggctgtgag acggtgaggc tgtaagacgg tgaggctgta
       61 agacggtgag gctgtaagac ggtgaggaat tgagcatatg agaatgaata accaaaaaag
      121 ttaggatgtt tgatgcgacc cgtagcatat tccctcacag ccttaacacc ttgccgcctc
      181 acccccccat tacggattga cttctctccg cgaaacgcac ataatttttc caatctggat
      241 gataacctgt cgcgcggcga taggtaaaga tgtgtggtag ggtgggttca ccgacaggcg
      301 ggtcctgttt gataaaggag aaaacgatgc gaacaagtgt gatcggaacg gtcatggtgg
      361 tctgtgcgat ttgcgtgcag ggggcgacga agttctggac gggagagggt gccgacgaca
      421 aattctcgac ggatggcaac tgggagggca tggccgcgcc cgtgaacggc gacacgctgg
      481 tcttccaagg agagaacgcc gtcgcagaga acgatttcga ctcgaccgtc cagttcgccg
      541 cgatttatat gtccaactcg gtaccgttca ccatccgcgg caacacgctt tatctgagcg
      601 gcaaccgaat ttttgcgaaa agccttcagt cggagattac tgaccgtttc gaagcggacg
      661 tggttttctc cggcggcgcc tctctcggag cggcgagcgt caaacagcac cacatcactt
      721 ttttgggaac ggtcgcgagc gcgtcgggag gaaccctgtc gaccgactcc caaaccggaa
      781 acatcttcac ctttgagggc atggtgacca atttctcaca tgtctaccgc ccgaacggta
      841 acggcacgct taggtttttc gcgccaacaa accacttcac caaccccagt tcgtttcaga
      901 tcaatcaggg gacgatcacg gtctcgaatc tcaccgcatt cggcacagat acccggacat
      961 tctccggcgg gcaaggtaga tacaacaccc ccgcaatcat ccagatgtat ccaacggcgg
     1021 acgagacggc accgatccat ttcaaagtct acagcccgaa tcagcaacag tccaacctcc
     1081 aactttacaa caacacgccc ggcgtgacgc tgaccttcac cggcgatgtg agcctgggct
     1141 ggacagtggg caatggcggg cgcatcgccg tcggcggagc gggcgacggg atgatcacgg
     1201 gggacttcac ctcgccgaat ctcgcgctct ataagagcga tacgggcatc tgggaactcg
     1261 ccggagcata cgcgtcgacc ggcgtcgtga ccgtcgcggc gggaacgctt gtggtcaaca
     1321 ccgtgctccc gacaaacacg ctctgtagtg tcaccgccgg cctcttgtgc ggcacaggga
     1381 cgctccaccg ggcgatacta agctccacct cccaaatcac cggcggcgtt cccggcatgc
     1441 cgggaacgct ccatttcgca aacggaggcg aggtcgcgct cacggtcgcg agcggcacgc
     1501 tcgtcgcccg gccgaaagca gacggcacgc cgtcgctcgt cgccgtcgac ggcacggttg
     1561 cgttgaccgg cttattgacg gtgaaccttg acggcgattt ctccgccctc ccggccggaa
     1621 cgtatccgct cctgacttgg acggaaaaaa ccgggacggg aacatacgcc gcaggcagcg
     1681 gcttcccctc ggggtcaagt ctcgtggaaa cggccgccgg actggcgctt tacgtgccgg
     1741 gcgcggttct gagttggaca ggggccgtga acggcgactg ggacaccgcc acggcgaact
     1801 ggaacagcgg ccaggtgttc gctgacggca cggacgttgt gttcggcgat gatgtcgtca
     1861 acggacagac gaatgtcacc cttaccaccg cagccgcacc gggttcggtg acgatttcga
     1921 gtgatgtcaa cggctatacc ttttccggtg ccaagatgac gggaacgatg acactcttga
     1981 aaacagggaa cgcctcgctg acgttggaga acgacaacga ctacaccggc ctcaccacaa
     2041 tcaacggggg cacgttccgg ctaaacggat cgcttgacgg ctcgcccctg ctcgtgcagc
     2101 cgggcgcgga actcatgcag gggacgaacg cttccatccg cggggatgtg aacgtgaatc
     2161 tcctcggaca ggcaacgctc tccggatcga acaccttcac cggcgatctg gtcttcggtt
     2221 cgatcgccga agggaaagcc acgggaacgg tttgcaacgt ctcggcgatc ggcggagacg
     2281 gcggtaatct cgttgtgatg cataacgcgc gagtcatgct ggatgcggag ggcgtctacc
     2341 ctaaccgcac gctccgtttc gcggcccgtc aaagcggcgc cggaaccgtg ccctacctca
     2401 agcagatgtc cggcaaaacc gcgacctggc ccggcggcat cgcatttgac gcgccggcgt
     2461 atgattattc cttcgagatt tccggaaccc tgaatctcgg caccgatccg gacgcggtga
     2521 tcacggacgc ggggagcgct tcggcttcga cgctcatggt ccgcggcacc ggacgcctca
     2581 atcagtacgc ccgggcgcaa ctcaagggag gtttcggaaa gacggataat gtgtcctggc
     2641 acgtctacgc ctccaacaac gtgctttcgc agttcaatat ctcagtcggc tctgcctttc
     2701 tgcacgcgcc gaacgcgctt tcgacgaact gcaccatcaa gctgggacag gtatacggga
     2761 acaataaatt caactccttg ttcgacttcg gcggtttcga tctggcgatc gccgggctcg
     2821 cggtcgatca gggaatctcg attgagggga acacccaggt gttcacaacg tccgaacctg
     2881 ccacgatcac actgctcggc cccggtgaca cgatcctgaa ccggaccggc accgaactga
     2941 agggcgcact cgcgatccgc aagcgcggcg cagccacaac gctcttcctc ggtcagccga
     3001 actcgaacac cggcgatttc cggctggagg aaggctacac cgttgccagc accaaccgcg
     3061 cgttcgggta ttccacgaac ctcttcgtgg gagcaacggg caccctcacg gtttccgcct
     3121 ccgacgccct gccgccggag gatgacgttt ggctgcgtgt ggccgacggc ggcaccgtcg
     3181 tgctctccaa cgacgtcgcc gcaacgatct ttggcctcga aatcaacggg cggccccgag
     3241 gtgcaggcac ctacggcggt gagggaagcg gtgcgcggat catcctcccc gccgtattca
     3301 gcggcacggg aacactttcc gtaagaaacg gcgcaggcac gatcttgcgg ttacactaac
     3361 gcggccggag ccccgatcaa ggcccgctgc gctttggcga ccgcgccatc catcagttcg
     3421 tcgatcactt tgcccacgtc ctcggtggac attttcgccg taatacggat catttgtcca
     3481 catcggtttt gccgaaaatc ccaagcggca ggaatgcgga cgcgcctgcc gcgtttcacc
     3541 cctctgattt tgtttgcgcg ccgagtttcc cggctggcgt ccggatgatt tcaaccatgt
     3601 tttccaacgc gaacaggatg atgagcacgc ccgcgatcgg catcgagatg taaaccatgc
     3661 cccgcttgat cggcagggca ggcaactgct gccccatctg ccccatcgcc aatccccaac
     3721 cgccgattac gaacacgatc agagccaggg caaggatcac cagttgcacg acgacgctca
     3781 tcgttttgcg cgcttcgggg tgcagcttgg acacgaagta gtcaacaccg agatgagcct
     3841 tcgcaccgaa cgccagcgca gatccgatca tggagatcca aaccagcagc acacgggcca
     3901 gttcgtccgt atagtgcgcc tgatgtccca agcagaaacg ggtgaaaact ccccacagca
     3961 ccgtcagcgt catgacggca accatcccca tcagcaggat ttccaacgtc ctgacaaagc
     4021 catctcttat cttctgccac agcccgctca ttccagaatc ctccttcttt gcaatccggc
     4081 ttttcagcgg cggcctcacg ccccgcttac tcgtgcgccc gctgacgcag catcgctgct
     4141 gcaatcgcga cggcgatctc ggcgtcaggc gaagcgtcac gcttctgcgc tttttgcgct
     4201 ttcactttcg cctgctcgtc cggcaggatg tgattgaacc ggggaatgat cttggaacta
     4261 agcgtcaaaa tcacaacaag tagtccgaga aaacccgaaa ccgtacccac ccccacaacc
     4321 atcagaataa gtccctgtaa cagcatgctc actcctttct gccggttatt tcgcaagcgc
     4381 aggcacgaga acgcggatga tcagccagac gacagcgcac cctgtcacga ggaacagcgt
     4441 gtacctccgc cagtcagcca tggtcgcggg agcgctgttt tctccgttcg cgccattaaa
     4501 ccggcggatg tcgatgtagc gggcagcgac cagagacacc aacaccaata ggtaggcgac
     4561 gtccgggagg gaggggaatc gtggacggtt ctgagataga taggcaagca agatcaacaa
     4621 tatcgcattg cctagaaaca tccagtaaag acgaatgaga caaccggcct cattgctatg
     4681 ctcgggaatt ggctggttct gcatgacgca ccatccattt cgtttgcgct aaaacgcgca
     4741 gtccaatgga tggcggcccg accatctcgt taacctgcgg gggctcgtta tccccccgca
     4801 cccggataag acacaacatg aagttgtgaa tacatgcact ataacagatt cgtaacccga
     4861 actgcaagcg cgtattcatc agcgatgcga aacgcaactg gaaatcctca cgactcccct
     4921 agacgtttgc gacaggcgag gcggtacaga ccgacagcgc acatgaaagg ggtgggaagt
     4981 gcgggttgtt gacgcagaac cccggatcgt tagcgataca gggtgcggtt gcgtatcagg
     5041 cgcgccatgg ccgggaacgt ttgatcgggt tgtcgagcac cgtgttaacg aggttgacgt
     5101 cctcgaccag ttggaaatcg tacatgccca cgcagatgaa gtccgcaccg ctctggaagg
     5161 cgtaaggaaa ggcatctttg gggtgcaggg cgcctgcggc gagaatcttg aaggcgatcc
     5221 acggctgttc gagcttctcc atataggcga tgacatcgtc aggatccaga caccagacgt
     5281 tatcccttga gtaatagccc gcttccgggc gggcggacca gtactcggta cggtgcaggg
     5341 tcttgaccca gaagtcgggg atgatgccgt tctcgacgca gcctttgacc gtttccagac
     5401 ggtgggctcc aataccgacc ggcacaccca gtttgcgcat gccctcgacc gcctcgccga
     5461 tcttgtcgaa ctggcctttc aaagcgagat tgtctgacct tccgccgtgg cagtaaaccg
     5521 ctgacgcgcc gttctcgacc gaaagctccg caccgcggac cagaccgtct ttatgtccgc
     5581 tgtcggagat gaactggatt ttgccgccct cttttctcca gtagtcgttg atgacacgca
     5641 tcagggccgg gttggtgctg atggtgttga ctccacaggc ctcggccatg cggaatgtgc
     5701 ggaagacacg ctcgtcggta tggtaggctt taaccagctt gtcggtgtag agcaggtcgc
     5761 gggcgtgtgc ccagcccccg atcaggttgc cgccaagcaa catgcgactc agtttcagat
     5821 tgccgatctg gccgaatttg tcgaccggct ttttcaggtc cttgatatca tcgaagctga
     5881 aggatttgac ggtggcgctg gtgaccgcat cggtggtctt gaggttctcg cgttcccagg
     5941 ctaggccgtg cttgcggctg aaggcgtagc cgaaggcacc gatcaccggc aacgagctga
     6001 ggctgagcaa cagctcgcgg cgtcccgccg aggacacccc taaaccgcta ccaccgtctg
     6061 cggcgatgtc gcggcggcgc aggaacaaac aaagacggtt ccataagatg ggaccgatct
     6121 gatgggaagg cacgagacag accgcgagta aggcggccgc ctcgaccaca ttgcggtcga
     6181 tccataagaa atgcccttcc gaggtatacg ccaggaaagg cggttgtgcg atgtaataga
     6241 aggccaacag cagcataccc gcgacagcgg ccggccggac cagtacgccc aagatcaacg
     6301 caaggccgat cagggtgagc ccccacatgt tggctaaatc gcagagccgc agcaaccacg
     6361 gagtgttggc gatatgccgg aagagcggcg cagcgaacca ttggctcatg cgcagatagc
     6421 cttcgcagga ccagccgccg ggctggatca acttccagac cccctcgtac aggaagtgcc
     6481 atccgatcag catccgcaac agagtcattc cccagttctt cttcatttta ccccgtttgg
     6541 tttgtccgga ccggctaccc aagttcaaaa gtgaattcac attgtatccg aacacgcaaa
     6601 acggacaagc acttattacg ccccgtccct tggtcgcaat aatcaggaat tcagcgaatt
     6661 gtcgcgcccg cgaaagcggc atgtacatcc gcttcgttca tttgcgtctc ccagtaaatg
     6721 aggttctccg ccttcagcgt gccgactccg ttagccagca caagttcaga tcgatcggcg
     6781 atgtcccaat aggccacgac acgacggccc tgtcgctcga agacgaacgc acgcacgcgt
     6841 gaccatttgt ctccggcgac gtcaagctga cgccattcga ccagctcgta ttcgttccga
     6901 ctgttccggt acagatgaaa ctccttcgtc gtgtccttga gaagttcctt ctgcccgggc
     6961 gtcagccatt tgcgtgcgcg cacgtcctcc caccggcgta tcgtctcgag aagatcatcc
     7021 gttcgccgat gcttcttcaa ggtgtcgagc gacaactgca tcgtggccgg acaatcgaaa
     7081 gccgcggcct tgctcgtccc atattcccag atatccggtt gcgtacccac cgtgcggctt
     7141 ccggatcgga aaagaccctc tttcgcagga ggcggcccgt cgagccgtgc gggccagcac
     7201 ccccaccatc cgaagtcgac gcgcgtaaag tccttcgcca gccgctgtgc cgcggcatac
     7261 gggtactcga tgatcttctt cttgaagatc tccggaccaa acacgtcgaa ggcgttcgca
     7321 ccgctctgga agtgccagcc gaaatggctc ttggcacacc cttgggtgaa gagcggcgcc
     7381 ctgcctgcgc gcgccgtgca ggcgttcaca cagcggtact gcgacagcga gatgttgacc
     7441 gtgcagggcg ggttcgcgtc ttccgaaccg tcgaaataga ggaaatcgaa accgcaggcg
     7501 tagacgccgg cgattttctc ggccacttcg tcctgcaagg aagaatcctg atccacgtag
     7561 gcgctgatcg ccgcgcattc gctcacgccc agcacgccgc cgatctcccc gagcggatgg
     7621 gccttcgcgt aggtgccgag gtggcggcgt ttcacgccgg tgaatgtgta ggggcgcgtc
     7681 gtggtatagc ccgtgtacgt catgagttcc gtgccgaact tcaaaatgcg gattttcgga
     7741 tgcatcggcg cggcgacggg gttctcctcg acgaagacct cgcacggctc ttccgagagc
     7801 ggcagcggct tcgcaagcgt gaagcgcttg acgagcgcca gccggtggtc ggcctcgggc
     7861 gtcacgtagg aactcttgaa gccgatgaac gtctgcaacg tgtgcaggcc gaccgaaatg
     7921 ccctcgtcgt ggaagcgttt caaagccgcc gtgagctttt ccgcatccca gccattatcg
     7981 aaggaataat cgggcagcgt attgtacgat ttggccttga cgatgccggg aaagtagacg
     8041 agcaacatgc ggaagccgcc tttcttcaga taggagagga gcgcgtcgac gttctctggc
     8101 cggaagtcgc cggcggtcca atagagcgag gcgttgaggc ggctgtcgcg gcggctctgc
     8161 acgccccgcg gcaggccgag atcgcgctcc acggcgtcga tctggtccag gaagcgttcc
     8221 ttctgcgatt cgacggcgag gacggcgacg ccgttcgtga cgccgtagtc tttgagcgac
     8281 tcgacggtca gcttgcggaa gccgtgccgg cgctcgttgt ccacatccat gtacggcgtg
     8341 ccgcccatga cgccgacgaa ggcctggtcg tcccacatca cgttcagcca gtcaccgaag
     8401 ttcgcgcggt tcttcaccgg caactgcagg agccggaacg catcgatcgg cggcacgtcc
     8461 aggttccagt gatagtactg gtgctcgtcc gtcgtgttgg agatgagccg ctcgggcctg
     8521 aatgtcgcat atccggccgc tgtctcggtc acgcgcacgg cgatctggta gggcgccgtc
     8581 tcgaagccga tgaagatgag atcaccctcg cgcctgaccc ggttcgccgg gtacgtcgtg
     8641 cgcttcgcct gctgcacgag gcggatctcg ttgttgaagg gccggatctg cgtcgacgcg
     8701 aagaccggaa tcccctcgcg cggctcgagc atctcctcgc ccgtcgcctt cacgatcagt
     8761 gacttggcgg tggcatccgg attgatgatg agcctgaacg tcttcgtctc gaccgtgacg
     8821 ggcgtcgtcc cgtcaaaagg cgtcttcggc atttcctcag cgcacgcgga aaatgccaca
     8881 atcgctccaa gcgtcaaata taatctgtct ttcattgtca tttcgtcctt tacggttaag
     8941 gtgattccga caaaacctcc atcagggctg caaacacctt cggcgaccga tccgaacggg
     9001 ggcggagcgt ctcgagcggc cgcttctgaa gctcatttcg caacacttcg ccagattcga
     9061 gctggagcgg gctcttcctg tctttggagc ggatgattgt ctcgatcttc gccgcccatt
     9121 catgcgcacg gggcgcatac cagtagcgcg ccagctcgta ctggtggctc tgacaatagg
     9181 tgttggcggc gttctcgaat aaaacggacg gaaaattagg attgcgcacc ggctcgattt
     9241 cattcaagcg caaataggat tcccagagcg aataatccgt atgcaattca agaagatccg
     9301 ccatacggtc ggcaagaccg gcaaggcggt tcgccgcggc aatcatcttt gcgccgtttt
     9361 ccgcgccgcc ttgccatttc tcaaaatcag cggccagcgt accggcctcc atcaccatca
     9421 tgcggtccag taccgtacgc gccaaatcaa tcgtatcgcg acgaacggcc tctccctgcc
     9481 actcaatctt ggccaactcg gcgaacacgc ccttcgcctc agcaagcctc ctgccccacg
     9541 tttcggcgtc cgtttttccg gcgggaccat attgcttcgt cccgaacgac ttgcgcgtga
     9601 acaggtaact gtagttgctt ccccagtcac tcatgtagga cacagggatg accttctccc
     9661 agagcgattt cattcggggc gccaacgcgc cataacgact gttgcagaat tcgtcaagga
     9721 cctgccggtg cgaaacggac gtggacgacc aggcgttggc cgtaaagaat cgcaataaga
     9781 gcgtgtccgt atggctggat tccggccaga agatataccc cacgcaagcg gagtcatcct
     9841 gcacgatttt ctgccgttcc tcgatgagcg gatagttcgc gcgtacgtcg agcgcattct
     9901 caaaggcgag gaacaccgag aacgtgtagg gaaacttgcc gacgacaccc cacttcgtga
     9961 agttgttgcc gcccagatcc agcatctccg gacgatagtc gcgcgtcgaa tctccttcat
    10021 agtcccagat gatatcccgt ttcgggtcca gcgaccgaac cagttcctga acttcctccg
    10081 gacgccacgt aaagtagaaa tcccatcctg ccaaaagggt cttcgcgttc ggatagtcgc
    10141 gatgcgcccg tgcgaggaac ttcttcagcg aagcgatctt gagttcaaga ttctcctgac
    10201 gattggtgta gcaacggcgc tcgccaaggc cgatcgtgtg tagaagctgc tgccggtctt
    10261 tcccttgacc atacgcctca acagcagtct gggtcagctt ccagtacgcg tccacccact
    10321 tgtcatcacg gacatcccag acgaggccgt ttttctccga ataaccggcc gttgtctgcg
    10381 gcaggaacgg cggattcatc ttctcgagga actcttcggg cgtgcgcgag taccaatgcg
    10441 tcatcgtacc gaagtcctcc ggaaccatca gtccgcgtgc gaacgcgtat tgctgaaggt
    10501 cgtgccgaag cttgccccgg tactccagcg accagaagag cgtccggttg tcgtagccct
    10561 taccggttcc cggcaggttc ttggacgcat ccggatagga aacgatgtca ggaaacgtgc
    10621 gctggaagag atcgtcctgt ccgatgcgga gcatgaagac gttgagtcgg cgcttgagac
    10681 accagtcgat ctcccgcttc cagtcatcaa gcccccagtg ctcggcttgg aaacgcgtga
    10741 gtccccggtg cgcgaaataa cggagaccgc ggtattcaaa gcgagcctcc tcatttacgt
    10801 cgagccccgt gaggtcgatg tcctccttgt gcggcaccac atcaatatcc cagaaccaat
    10861 gacagccgcc gcgacgctcc agcaaatcat acagtccata gaagagggac cggacattgg
    10921 atccggtaat gacagccccg cctgtttcgg atctgatcgt ataggcgtca tttccccttt
    10981 tcgatatgga acgatcaatc gcgaaccgaa cggactcctt cgttggtgtc ctgcctgtta
    11041 tctgccggta atatttaccg tactccgccc tcgccaagtc aattccgtcg gcaaacgata
    11101 cggaaacggc gatcatcccc gacaagatca aaacgccggc cttactcaaa ctaaacataa
    11161 cctatacccc tttccgcctc gtaaccatcc gccgacatcg gcaggcaact acttcattct
    11221 gattgacgtg ccgccgggaa cgatcttcac ggtcagacta cctccggctg ccgacagcac
    11281 gatgttttga tagcggcccg gcagtcccgc tgtgtcaacc gaccagttcg agaggtcaca
    11341 ttctccaata cccgtcaaaa ggacatacga cccataagtc aacatgttcg tcgccccggt
    11401 gaacgttacc gtacccccct ttgaaaagtc aatcgtcccg tcgaccgtca caggcgtcac
    11461 cgtaccatcg gcattgacgg cagcctcaag atctgcggcc gattgaaggg cgaccccacc
    11521 gctgatcgtg ccacctgcca gcgacagcgt ttcgaccgaa agcggcgcgt tgccaacgac
    11581 cttcagcgtc gccccgttcg acacggcgac cttcgacgcc tttgtgccat tccaccctgg
    11641 cgtcggctga ccgaaccatt tctgccgaag atatgcctca acaagggcca cctcacgcgt
    11701 cgagagctgt ttctcataaa gcatgtactc tgcaaattgc tcgccgccag catagcgggt
    11761 gctgctcgca acacccgtca gcgccgatcc gccgaagttc tcgtaagacg cccacgagac
    11821 gatgttgaag cccgcagaga gccccgtctg tcgcggatcg caggcaactc cattcttgtg
    11881 gaaccgcacg ccacgctcat ccattgccgt cttaggcata agcatgtcgc ttcggacaac
    11941 gggctgatcc ctgtcccccc ccagcggccc cgatgtctca ccagcacgga cgattgtgta
    12001 taccgatgac cagtactggg agtcaggcgc cgacgttccc accagattcc cgccgccatg
    12061 atccgctccc caaacggcaa tgaccgtatg aagttgtgga gtggacgcat aagaaagcac
    12121 gggggccgac ggggcgtact ggttggtcgg caggccaccc ttttcccatc gctttgcctc
    12181 accaaagtcg acatagggca ttccgccaag cgccgtactc gtccgcaggg ttggataatt
    12241 cgtgcaatag tccggatgcg aggccggatg ttcaccgctt gcagaatcca gccatttcaa
    12301 aatgtttgtg acgccgttct ctaccgtata ctcgaggttg ttgagattgg acgcatcata
    12361 acgcgcccac gctccggcgg gcaacatgtt ctccgttgcc gcaacggatt ggatttcgag
    12421 tgtgccttcc ttcaccaaaa aggaaaccga accgtcctcg ggcaaggccg gcacgagcaa
    12481 ggtgccgttt cctgtcttgg caagcgtacc cgcaccggtc acccgatcaa ccatcagttc
    12541 tgcgccatcc ttgacaccga tgccgccgct gccctcgccc aagttcaacg tgcccagcga
    12601 agcttcagaa ccggtttgaa tcggcgcata acagatttcc gtcgtctgcc acttgcgcgt
    12661 cagataggcc ataatctgct tgcactcgac gaccgtcaac gcatttgtgt aaacgatcaa
    12721 ctctcccagg cacttgtgcc cgattcgacc ttccttggcc cggacagtgt tgtattcgta
    12781 cgagtccgct ccaggcaatt cataattggc attgcactcg aacgccaaaa caatcgggac
    12841 aaaatacttc accgaggcat tttcataggc gggataggga tatccctgat ccggattgct
    12901 gagaacgcca ttgatccaga gtttcccctg cttgatggca tctgttcccc agtcgaaaat
    12961 cttgccggta tagacgttgt aatgagccca ggcttccgcc tccgtgccgg tctgttgcgt
    13021 gtcaatgatg cccaagaaat taccccccga ccgccaacga tcctgcgcca tcacctgaaa
    13081 gacatggcgg atgttcttta caggtttact ccagcggtgc gtgtcgctat ccgcaggaga
    13141 tgaactgcca ctgacgaaat taccttcgaa atgaacatac tggatgttgc gggtcgtatc
    13201 cgtcctcagt tcaggaaaat agcccgaccc cggtgcggaa cacacttccg aataaatgta
    13261 atcaccatca ggatcgtttt tctggcgaac atcctcgatg cgtgtaaccc ccttcacacc
    13321 atttgtgaca tcaacgagag tcattgccga accgttcttc gagcagtcaa cccaaaacgc
    13381 cgggttgccg gggaccgtct cgccgccttc agaacggatg atcgatccgt cacactcgaa
    13441 aacgatttcc gcgccatcct caagcgtgag tccgtctatc gaggccacat tggaaaagaa
    13501 aagtttacca gggcccttca caacacctcc ggccagactg ccgacacggt tcgtgacacg
    13561 aaggccttgc acaggcagtt gcagaacggc attcgacgtg atcgagaacg agccgatttc
    13621 aatcggacac gactcatctt cagccgttgg cacaaacgtt ccttcttcga taaccagggc
    13681 acccgtatgc ggcccctcgc cgaatccgac tcgtaacaaa ccggcccccc ccttggtcag
    13741 ggatcccttg atcgcctgga attcatggat gacggcctcc atgttgcccg tcaccgagaa
    13801 gcgggaattc tgtcccgcat ccccttgacg caccgttgca tagggaaggc caggaccatt
    13861 ctccgtgtta ggagcatcag agaagaggat atcacatgtg ccgacctcaa gcgccgtatt
    13921 caggactttc ggtgtgtcat ggagaatcag gcggccggga aatgtattgt tgcaatgaat
    13981 gcgcgtccag cggttgccac cgcttaaagt caacaatttg agcaatccaa cctcaacctc
    14041 atatatatcc tccatgcgat acttgccaag gtttatggtg aagttgtggt ctgtcgaacc
    14101 attatgcgcc agctcattta cataccggcg gcagtcgcct ttccgtccgg caaaggaaag
    14161 ctcagcagcg gccgccgttt cggaaaccgg ctgcgaaagt tcaacaaagt catccttgcc
    14221 gaagatccgc ctgagaaaag tgccgcccga aactcctgca cccgacacca gagcgccgat
    14281 cggcagggta tcggccgcac cggccgttac ggccttaaca aacggcgaac cggcttgcgt
    14341 cgagaacttt tgagtgatgg ccgcggaact cccgcgcggc ccgttaaatg acagcgagcc
    14401 gtgatactca gtgatcgtcc ccaagggaga agggcgccag gtggtctccg atgacggcgc
    14461 atactgatta tcgttgatgg aaaacgtacc gccatggaca aggattttcg ccccgttgcc
    14521 aaaggtcatg tcgccggcaa gtgaacagtt cctaagataa ggcgtactcc ccttgatgtg
    14581 aagcggggca aagaaggcca tgttcttcga gaacaaattg gatacgattg aaaattggtg
    14641 atcgctgatg taacgagtcg ttgaaccggc attctctacc cgtgcgacgg tgacgggcat
    14701 cgtattcgaa acccatccgc cagaaacaga aaccagagcg tcatcgctgt tggggatcga
    14761 cttggggttc cagttgtcct catccgccca atcgggcatg gacaccgggc ccgacgcgct
    14821 cgatccggtc caaacatagt ccgtcgcagc aacagccatt tgcgcaaaag cgaccgcgaa
    14881 catcgccatg cccccgcgca ccgcgcccgc gcttcttccc ttaccacgcg tttgagacag
    14941 tttcatgttg ttgttccttt gttttggttt gtcctgaccc gcactccaca caacgaaacg
    15001 accgaactca gcctttaccg acattcttac ttcagacctg ctgaagtgat ctcaaaaatc
    15061 gcaaattcac ccggtccgag acggcaatcg aacgaaacgc cagccgacac ggcgacaact
    15121 gcttcgctca aaagctcacg cacggcgcag aggcgtttct tatcggactt aaacgtgatc
    15181 cggatatccg atgtctttgt tgcgtcaagt tcttgcggag catcagcaaa cttcgtcacg
    15241 ccgtcgttgt tgaagacata aagccaggag tgcctgtcag caactgtcaa tccgtacatg
    15301 cagtccccct cgacctcaaa cgggaaatac tcggcctcaa ggtcgttaag cgccttctcg
    15361 agtttcggat accgtgtctc gccgtttccg atcccccgac cctttccgat cttgaccgga
    15421 tcggccagca tatccacacc gacatgaatc accttgccgc catccgcctc atatctggca
    15481 agcgtttttt cccaagacgt gtcgggatag tcaccggcga cgacgagcgc cttgtagctc
    15541 ttcatgacgg ccagcaactc ctctggcttc tggctctttg catccggcgc aatcacatca
    15601 tacatgttcg catagggaga attgcgtaga catttctctt cgccgcgatc gcgacaagcg
    15661 gtatagtcac agccgggtac gagcgtaaag aagacggcat cgaccgcctt gtcgccttcg
    15721 gtgtaaccga acgagggatc ggcgaacggc gccccccccc caggtcggat agccctgcgc
    15781 gagcggcaca caaatggcca ctggagaata atgggtaccg cgatcgggat gatcctttat
    15841 gaattcggca aatgcggcat agatctcacc acgcggcgag aagaccgtcc tgcctttctc
    15901 tttgtcccac atgttgagga taccgcagtt ccactcctcc agttcaatat agtttgcacc
    15961 gctcaggtag gcgagataat gcgtgcgctt gaagagggaa cgcgaaatgc cgaagtccgg
    16021 cccgctgttt ccgatgcgga tcttgccgcc cggacaccac gatgcctccc gctccttcgc
    16081 ataacgcggc tcgcctgttg cttcgggcgt cgccggatag cggcacatcg catccccgag
    16141 aaaccccctg cccttggccg acgggccgtt ggcatagccg gcgatatacc attcccacgg
    16201 tataccgaac tgccgcgccg cgccgcgcgt gaacatcgcg gagggctggt agcggtactg
    16261 gcccgaactc gtcgtctcca ttcgcaggag caccgccccg aaatcggcgg cgatgtgcag
    16321 cgaattgagg tgcgcgtcaa gtacggacat cgttccgccg tagttccttt ccttgcgcat
    16381 ttcataatag cgcttgagca acgcgacatg ctcatagcgg tttttcggtt tttcgccgag
    16441 gaacgcccgc aggatcgcca cgcggtttga cgaatcgggc cgatggtcaa cgtatgtgcc
    16501 cttctccccc gccacggaac tccatgcgtt attgaggtcg ttacaccatt cgctgcaggc
    16561 gccgtccgcc acaagattcg gatgcgctgc ccgccaggcc gcatacgccg ccttgtcgag
    16621 ctgcacggga cttccccacg tcccgcggtt gcgttcaccg cgtttggaga gaaaaaacgg
    16681 acgatccgga tgcgggttcg aagtgaggat ttcacggtct ggcgcatccg gtctgaaatg
    16741 gagatccagt gtcggccaga cggcgtatcc ggtcgcggcc gcaggcggct ccgccgctgc
    16801 cacgtacttc ggcacgacat cgctgatggc taagctgttg ttgaactcag gattgtccgc
    16861 agccagcagt ttgccggacc acccgtaggg agtatatctc gtcaatacgc gccagcagtc
    16921 gcggcccatg atctcaagcg gcttgtgccg cgccggcacc tcgacaagcg gcacgccaca
    16981 cacatcccgc tcatcgcaac ctgtcaatac aaccaaccct aaccccgccg cgagcaaacc
    17041 gcgcggaaac acatagcgga cctgtgcatg tttcatgctg ttttcctcca ttgattaaga
    17101 atcggacggc agaatttctt cttgcctttt tcacaacttg agtttgctta ctcatcggat
    17161 tatgacagcg agccccgtct ggctggacac cttcgcgatc aacgcaccga cgtccatcat
    17221 gtctccagcg ctctttgcgt actcaaacga cccgtccccg tccggcacac cgagaggcta
    17281 tccttggccg tagcgacagc cgcctgaatc gttctaaacg ccgtctgctc gctcgttccc
    17341 gatccggaat cgtccggtcg cctccagtcg acaaagtatt ccgtcgcgca ggcagcggtc
    17401 gccagaagtg acaaaacaca ggcaaacagt ggagattgcc tgcaaacggc acattggtca
    17461 tgtctgtttg tcatgttgac tcctttttca tttgcgttca tttcagccgc aggacgacga
    17521 taccggcagg cgggatcttc gcgacgagtg acgtaagatc ttccgccttc gccgagatga
    17581 cgatgggcag tgatggcgat tcggtcaggg cctcggccgt cgtaacggga ccgcgaacga
    17641 gaatattggc gttcaggtag gtcggccggt cattgaccgc gaagacgatg cgcgaaccat
    17701 cttccgcgac ataggatgaa agacgcaaat cagccatgtc agctccgggg aagaccggag
    17761 aagctcggtt gaccttttcg gcggccgcgg caagggcctg agggtcgaac gcccgaaacg
    17821 gaagcggatg acgccaggac gccgcatcga ggacggacgg ccgcggaccg aacggacagg
    17881 cgggagaacc gtaaccgaag gtcacgactg ccgcggagcg gtttgtgaga gacgcgagct
    17941 cgtcagccgg aaagcgaccg gaattcagga tgaggagcgg gaggtccgca tcggccttcg
    18001 cctcttcaac cgtaacagcc gaattgaccg ccgcgccagc atttacgaga tgggcgagaa
    18061 gggtgtacgc gctcggatag cccccgctgc aataggcatc gaattctgcg tccatcgcat
    18121 gtgacgagac gaccacacgt actccgtccg cccgtttcgg acacttcgca aggttccaag
    18181 ccgtgtcgag gcgacgccac tcctcacgcg taaagccgtc cgtcaggcac cacaggacat
    18241 ccggcaccat caaacggtct ccccgcagga gatttcccag caaaacaact tcagccgtca
    18301 cgcgctgcgg cgcatcgcgg agcgaattgt actgttccat gccatccttc acgcacgcaa
    18361 gatggatgat gggcgtatcg acggtggcac ccgtcagcaa gaaggctaca cgacagctat
    18421 cgagcaacgg gacaggcgaa aaccgccagc cctcgagttc gagaacgatc gccgaagcct
    18481 cagccatgaa accgtccaac cccgccttct ccatgagccg gtagtcgata ccatgccgat
    18541 acagcgcctc aagcggatcg cgcgtccaag acgtgttcgc atagagcttg agccccctcg
    18601 catgcagagc cgcggcgagt ttagcaatga atgcagcatg tcttgcggca tgcgcccggc
    18661 agagggacgc acggtctttg gcctcgggga agaggtgcga atagtcaagc tggttcagcg
    18721 gataacgcgg atggccaaac ccgtcgcagg catgcaagcc ggcgaatccg taatctgtca
    18781 agaactgcac ggcctgtgcc acaaagaagt cagcatagtc ccttccgtcc gccagccgct
    18841 cgatcgggtt tacattgttc gcccgcctcc cgtccttcat ccgatagccg acctctggat
    18901 gacggtcgaa ccaggtcacg tccttacgcg tcgccttgaa ctgacggagc caggagttcc
    18961 ccttccctgg cgccatgtcg aaaaatgaag gatacgaggc gataccgtac ttcttgagtt
    19021 cggcgataag tccgcggaat tgccaagccg tccacttctg ccgcagacgc tcctcgttcc
    19081 acggacgcgc aaaatacgaa cagtggaggt cgccgatctg gaaatctgac gagagatccg
    19141 tatgcgcgtg aaaaagctca ggatcgaaga taagaaacga aacccccttg ggcttgacgg
    19201 acatcttgtc aagatatgcc tgaaccccat agtcgggctc cgtattatca aaagcgatga
    19261 gttccgtcca aagatacttg tccgccccgc atgtacacat ggccattccg acaagcacta
    19321 ccagtgccag cagtgctgat cttcggacac tcctactgtg gtctaacata ataatatttt
    19381 ccttccaggt tcttgaacgg cagggttgca aagtcgttgg tcgccgtccc cggcgtcatg
    19441 cagaagatct ggctcgcagc gagttccgcc gcgcccggcc agagatagcc ctgcttgacg
    19501 acgaccgcgt catagtctgc ggcggcaacg tccatcgccg cgaaatgctc tttcatcgtg
    19561 aaagacgtcc tgacagaggc gaagacgatg tcgacatgac ccgctcggaa gagaatgcct
    19621 tctccggact cttcgcctac gaagccgagg atcttggccc cgtgaatgta ttcgccctcg
    19681 atcatgaggt cgtcgttata ctcatccgac ttcgggatcg tcaacgtcgc gcgatcgccc
    19741 ggcttcatct ctgtgagctt ggcccaagct gtcggacaat agagcgaggt aaagagtgtc
    19801 tttacatcgc tgccgaggaa acgcttcaga agaaagacac tctcgccgcc cgcgccggcc
    19861 gtcgtgttgt cgcccgagtc gctgacgaat accggctttg ccatcgtctt gacggcatcg
    19921 accgtcctgt cgggcgccag tgccggcacg ccgaacttga gatccgtctt gtgtgcatcg
    19981 aaatagtccg cgatcgcttt cgcctttgcg tgcacggcct cgtgattcgc ctttgagtaa
    20041 acgatgaccg aggccgacat gcacggcgcg tccacccacg gctggccgtt gaagaccgaa
    20101 gccgagatga tatccggatc ggtatcgagc tcgcgcacca tcttcatgat cgagacatac
    20161 ggctcgaccg aggtctgcgc ggcatccgcg aactgaacct tgatgcggaa gaaatggggg
    20221 accgtcaccg ccttctcgtt caggatgcgc atgagcgcaa ccgccgcgcg cgtctccgtc
    20281 tcgtcgtagt ccgtgtgcgg tgccgtgcgg tagccgctga tcgcctgcac gttcggaatg
    20341 agcccgtccg cgaggttccc gtgaaaatcc tgcgcaagcg agatcgggat ctggtcgccg
    20401 atcgtcttgc ggagttcctt gaccgtgaac tcctcgccgc tccccagccc ctcgacgcac
    20461 atcgcaccgt ggagataaag gtagatccct gcggcatcgg aatgcttcgc cgcttccttg
    20521 cggaagtcgg cgagcacctc gtcgaagcac tcccgcgtca ccatgcccga cggcagggaa
    20581 acggcgtaga cgagcggaac gacctcgaac ccggcctctt cgaagagatg ggacgcgaag
    20641 agtttgttct tcgcctcctc tccgtggagg atgtagaagt tctccttccc cgccttcggg
    20701 ggcgagaagg tgtttgattc gcactggaat gatccgacga ggattttcat tggcttttca
    20761 caattagtaa gttgagcgtc ggaagtttaa cgcagagacg cagagatttt tacgggatgg
    20821 cggcgtgacc tccgccgggg gagggacgag cgtctgctcg tccgcgggca agcagacgct
    20881 tgcccctccc gatgctctgc aatgaaccct gaaaccaaga gaagtgtacg catcagcctt
    20941 tcgcaagata cgctgttgcg ggcggggacg cccgcgctcc cgactctctg ctctcctttt
    21001 ctccagaaac gcatgatctt cgcacttttt cgaaatcgct caaagagaac gtgaaaagta
    21061 cgcgtccatc ttcggggaca tcggcttgga gccgttttcc gtgatgatgt agccggtctc
    21121 gatgcgcgcg ccgttgaact tcggatgccc gaagaagctg acatcgacca tgacggtcat
    21181 gccgggtgca agcacatcct cagagttcgg cccgtagaag ggcgcctccg cctccatgag
    21241 gccgatcgtg tgggcgaacg ggcagacgag gtacttgaag aggccgtgct tcttgaagta
    21301 ctcgcggccg ggcacgtcga tctccttgcc ggagacgccg ggcttgagca tgtccttcgt
    21361 gagccacatc acttcgcgga ggtggttcat gcactccttc tggtcctgcg tgaagacgcc
    21421 gctcacgggg agcgtgtcgc cgaaggttcc cgcatagccg ttgaagcgcg gcgcgatgcc
    21481 gatcatcacg agttcgccgg ccttcatctc cttgttcgtc gccgtcggca cgaccgcatt
    21541 cgcgcgcacg cccgagccga cgatggtgga gtacgcgaag ttcgtcgcgc cgttcgcgcg
    21601 gcagaccgcc tcgcccgcag cggcgacctc gtactcgaac acgcccggct tgatcttcgc
    21661 cttcatcgcg tcatacgcga ggtcgcaaag accgacggcc ttgagcatgt tctcgcgctc
    21721 ccactcgctc ttgaaggcgc gcagcgcctc gtagtcggcg gtgatgtcca caagctcgac
    21781 gcccgcgaac cccttctgga agtcgagata gacctgatgc gggatcgtgt tcgtgccgac
    21841 gagtccgatg cgtttcaaag gcatgcccgc ggcggacatc tccttgtaga gatcctcgaa
    21901 cccgatgatg tgcgcgttcg gatactcctc gcccggcacc atgaacaccg ggaagcagcg
    21961 gatcttcttg atcgccgaac tctggatcgc gaacggctcg gactcgggcc cgccgaggag
    22021 ccacggctcg cccttcgcgg gaacaaacac ggagcccttc tcgaactggc cgcaccagcc
    22081 ggtcaggtac cagccgttgg cgacgttcag ttcgtcgaaa tagacaagcg cggcatcgag
    22141 gttcttttta tcaagaatcg cctgcacggc cgcaatacgc ttgttcgtct cgttctgatt
    22201 gtaataggac acttttctct ccttagcagc taaacccttg aactgggtaa tacgccatgt
    22261 ccttccgacg gtcgaggcga ccgtaaacgg ccacgacggg cacatccgac gcgagaatcg
    22321 tcgcggtctg tcccggtccg agctggtact gctcgtctcc gagcgggtaa tccatacgga
    22381 agcacttgac gcggcgcgcg gggacgtgca gcgttatgtc ctccttcggt tccttgtctt
    22441 cgaagaggat tgtgactttg attgtcgcgt cctgctcccc gttgttgacg atcatcagcg
    22501 cctcgtgtcc gaacggctcc gaatcgcccc gcggcggcag atcgccgtcc gcaaacaccc
    22561 actgtttcct tccgatttcc atgcttatca cagacataat ctctcctttg cttaactata
    22621 atttaagaaa agcctttgaa gccttcaatt gtaaaatctg caacaaccgc ttaaatatct
    22681 gcaacaaaaa tcaacggtag tgctttgcaa agatcaagaa gacattcgcc tgttccggat
    22741 catcactcga aaaggcaact tttcgagagc cggtgaacgg agcaaacgga cggatctttc
    22801 ctgaagcaac gatacaaggc ttctcgccgc ggcgtttcag ttgccagtgc tcgccatcgc
    22861 ggcagacgag atactctttg ggggacaacg tgcagtcgaa ccgacgcgcc tcatcgccga
    22921 caaccagcgt aaacggggct gtcggaccgt agacctcaaa ggaaagagca gccttctcac
    22981 ccggacgggt cacgaggtcc ggtatcttct caaaaccctt ggaaggcgcc cacgtcgccg
    23041 gttcaacgac ctcgtaggcg agagcctcgt gcgccgcggc gttaaccgtc ggcttcagag
    23101 ccgccgcagg ctcgccaagc gcaaggatat tgacttctgc gcggacgttc acgccttcac
    23161 cgccgttgcc ctcgaaagta agttcattcg tgccggccct gagttcggca aggtctgccg
    23221 gtgcccgttc caacggatta gcgctcgcat cgtaccgggt ccaacagccg tcttccatct
    23281 cggcaaagtc ggtcggttcc agtgcaaacg gacaggcata gcgagtgccg ttcaacgtga
    23341 cagccaatcc ggaatacgtc acttgcttac gctcgggcaa cagcttgaca gcactcactg
    23401 acacctcagt tttgccgccg gccgggatct cgttcaaata gagattcacc gcctgcaact
    23461 ttgaaacatt cacgcgctca agcgaataat cgatcgaaga atttttttcc acatagggcc
    23521 agacgaagtc actcgccgca cccgaatccc gttcacggcg gttgaactcc aaataacgcc
    23581 agccagtaaa gtcgagcctc tgatagtgtt cgctcttcgg tccgcttccg gcccctttcg
    23641 cccccatgag ctgtacgttc aagagcgcac ccgaaccatc gccccgtacc cacagcccga
    23701 acccgcgcga accgctgcaa tcgccataga caggcggctg gccgacgggc ggcttcggcc
    23761 agacacgcgc cacacacgac caagcaccgc gtgacgtggc gttggcgttg tttgccgtta
    23821 tcgtcaccac atgaccacgt tcggaatcct gagagttcgc acattcgacc gtcacaccct
    23881 tggcagcgga tttctcgaag gtggcggcac aagccggatc aagcaaaact ttcgcctcgg
    23941 gatcatccca cggcttgcat gtcaacaacg cctcgatgcg caacgtcgct tcacgccgtt
    24001 ccttcgagtc aatcctccat gcgcgcgcgt ccggtcctgt cacgcgatga tgaaagaagt
    24061 caagcgggcg taccttccag acgccatccg tatcctgccg caaccgatat tcgtcgcgtt
    24121 tgacgcggaa cttcgcaagg gtctcgtcat tgaaggcata cgccagccga aaccgttcat
    24181 accatccgat cacggtcaca taacgatgga tgttgcgcgc caccatgtat ctggcggggt
    24241 aggcgtttcc cggcaggacg ctcatcgcgg cgtcataggc tgcgcacttg cggccgaagt
    24301 attcgtgttc gtcaatacgg tagggatttc cctgggcccc gtttccctgc aatgtcgccc
    24361 accaccccat ttccggctcg atgaagttgg cgtcacgcgc ttgactgaca agccgttcga
    24421 agcggcggtc gagagagcgc ttggaacccc attgcacata gtcgttggca ccgatgcgcg
    24481 aatggaacca ccaggccgcc gacgtccaca ggctgtcctc gaaaagcggc gttttgccgt
    24541 tggcctgcgc ggctttgtag agttcgcgca cgaacttgtc ggtcttgcgt ttgtcgcgaa
    24601 tgcaatcggc accatcaagg tagtagttgt caaaacccgc accgaaaagt ttaccctgaa
    24661 gttcccacgc gcgcttggcc aattcgctgt cggcgtcaat ctggaagcaa ctaaagcaat
    24721 taaagaggta ctcgaccttt gtgccctttg cgtgcggctt gaccgttgtg ccgaatgccc
    24781 cgcgcgtgac acccgtgaac gtccacgggg cctccgttga acggccggta tacgtcacca
    24841 gttccgtgcc gatcttcaag gtgttcgact tcccccaggc ggttgtctcg aattcgtgac
    24901 aatcaaccgg cttttcgttg acaacgagcg ttgtcacgtt cgtggcggcc tccaggtcgg
    24961 tgccgagcgt gtacgccgcc gtcaccatga gtccgtcctg acactccggc gtcgcgatgg
    25021 gatcgttgcg cgcgacacag gcgcagagcg aatgaaagtc acaatgaagc cccgcgtcat
    25081 gagccttggc gatcatctcc ttcagaagcg tcatcccgtc acggccagag ccgtcgccgg
    25141 gcagctcata actcccgtag gatttgaaga agtgatcgaa atgcagcgtg ccgaagccac
    25201 cgcgtctaca ttgttcgatc tcatcgtcga tcgtaaatgg accgatcccc caggccatcc
    25261 aatacgagcg ccgacacgct tccgcaccaa gcgcccacgc gccgcccctg tcgctgcagg
    25321 gcacgtccgc gtcaatcgtc atcgcgcgca gcgcaggtat cgccgccgaa cgcgggcccg
    25381 ccgcaaaccc gaagcgcgcg cctgtcagcg gacgcgccga tgtctttacg gacgcctcca
    25441 ccaggccttc ggcacaacgg aacgaagctc cgacatcata ggcgcgcacg cagacgaacg
    25501 accgttcatc cgagtaggcg ttcaccatgt gcccccgata tttcaagcag tcggggtgca
    25561 ccctgccgat atgaatctcc tgcgcatcct gtacggtctg ccgttcgacg ccaagcgacc
    25621 agcctcccga aaacgggcgc accgccaaca ccatctcgcc agcctcagcg aagccgaaac
    25681 acaggttccc cttggcatcc ttcgcacatg atgtcggcac gagcgtcttt ccctcggcgt
    25741 tcacaaccga cacgaaaggc gatttttcgc ggacaagctc gcggccgctc tgacgctccc
    25801 tgatcgacgt aaggcagccc tgcccgtcaa aagtcagcga gaccagttcg ttttccaaca
    25861 tctcgtccgc caaaaccgat ccgattatca tcgcgatcag cgttgtgccg attttacgta
    25921 ccgtcatgca cttacctcct gtaggagaga ccgttaacat gccgcgcccc ctggccggca
    25981 cctagtatgc ggaaccagcc ggatcgtcac ggcaggcgga ccggtttggc ggtgtggcgg
    26041 gtcaacgcgg caacgatccg ccgaccgtat tcgggcggcg gctgcggcgc ctcgtaaacc
    26101 ttcagcccca gacggcgcgc cttgtcgatg ccgtagggaa tatacggcgc gacgtgcaat
    26161 tcaatgacgg cgggcagcga atccgcgagc cgcccgacgg cctcgatgtc ctccgccccg
    26221 tcgttgatac cgggaatcat cgggcagcgc aacacgatcg agcgtttccg cggagactct
    26281 gcggcgagtg cgtcgttgac acggcgcagg ttcgccaaaa tctgcgcgag aggcctcttg
    26341 gtgtacgtga agtgcttgtc ggcatccagt tttttcacgt cccagagcca cagatccaca
    26401 agcggcagca agggctcaaa gaccttctcc gtcgcggccc cgctcgtctc caccgccgta
    26461 tggagccctg ccgccttggc cttgccgagg agttccgcga gaaacgcggg ccgggccagc
    26521 ggctcgccgc cggacacggt gaggccgccg tcggacacgt cgtagaagtc cttgtccttg
    26581 agcacttcgg cgagcacgtc ttccgacgac atctcccgtc cgatgacctc gccgtccgga
    26641 tagcgttcca cctcaaagct ccagctttcg ggactgtggc accacatgca ccgcaatgga
    26701 cagcctttga gaaaaaccac agtgcggatg ccggggccgt catgcgtggc gaacttctcg
    26761 atgttgaaaa cgatggcacg tttcatccgg tgttccctca atgtaggcga tggcgctgga
    26821 tcacctcgtc ctgccagcgt ttcgagagcg ccacgaaacg cgcggagaat ccgcacacct
    26881 tgatcatgat gttttggtgc cgctccggat ggacttgtgc gtccaaaagg tcttcaacgg
    26941 aatagcaatt cggctggaga aggtgcatcc ctttgcgaca tgcgaccctg aacacggccg
    27001 cgaacagttc tgcgttcatc gccgactttt cgaaagcaag gttggcattt gacgcataaa
    27061 gccgcgaatg gtcaagagag ccgatggcgt tgaacaccgt cgtgacaccc gctttgcacc
    27121 ggtattcact tggcgcgaac ccttgcgcca agcggtcacc gtcataacgg ccatccggcg
    27181 ttgcccgcat cttcagcccc cagagcagga attcccggta tatccagcag gcaagcacat
    27241 acggtccgcc cttttcgttt ttcaagtctc ggatgtcctc cgacacggag tcgatccagt
    27301 atcgcatcag tccgttcgac gcttcgctgt tgtcgcccca atacggtgcc gccaatactg
    27361 cctgacgcaa cgcctcgcct cccggacact tccagttcga cctgacggcg gccaaaaagt
    27421 cgggcagcgt ggccatacga tcctcgaaac agatctttcg tattgcgcaa agcgaatcca
    27481 cgacgttccc caagaaggcg agagtgatga tacgcggact gaaagccgct ccgcccgcca
    27541 gggcgtcacg ccgcgccgcc acgcaacccg tcagacacga agaatagacc ggccgcgccg
    27601 acacatggac gttttcgccg ccataccgcg tatagtcggc aagaacgtcc cgcagaaaac
    27661 gaatgaagtt gtggtaaacg atatcgcgca cctgttcgaa gcttgtcgcg tcgtcaaccg
    27721 gatcaatcgt gatccgcgcc tcccgctcaa aattcggatc acggtagatc gtcagttcga
    27781 ggatacgcgc aaccgacatg taattggccg tgtcaaggtt tgtgtcgctg tcgacattgc
    27841 cgtcccagca accgcagcaa acataatcgc gggcgcgcgc cacgggatat cccaaggcca
    27901 tgaagttcgg aatgtgccgg tcgtcgttga agacggcaaa caccgcatgc ccgcgcatca
    27961 gcatcgcgcc gatcttctcg agatattctt ggggcgactt ctctgaaatc ctgcaatgaa
    28021 gctttgggaa cacaaggtcg agcccgatat gcgcgtccag gaacgcagcc gtcaattcgt
    28081 tgtagacagg acgtccctcc gcatcgcagc ccccgagcgt cagggggatt tccgcctcct
    28141 gatccgaata gccgtcgatc tccagccgtg tatcgagatg acagtcggcc atgacaagaa
    28201 aacgggcgat gagatcgcgc gcctccggct ccgttagccg tccggcggca agatcgcgcc
    28261 gatagaagtc gatcagccat gcatcgggac gcccgaggga aaaattgagc gttccctcga
    28321 cgtacccgag gatctcccgg acgaaccaaa gggtgttcaa tccctcgtag aaatcgcgcg
    28381 gcggctccca cgggcaccga cgagccgcga cggcaatccg ttccagtctc aaacggtctt
    28441 ccgcagacag gccgacggcc gccaactgtc tctcggcctc gcgcacaaaa gcaagctgga
    28501 tctcatgcac cgtatccagc ccgacaagcg ccacttcaag ccccttacgc ccggaggcat
    28561 cttccgccgg acaggatgcc agcgcggtct ccacttctcg acgaacgccg cgaaagcctt
    28621 tggtgaaaat cgtatggaac ggcggaatat gatgcatgtc gtcgacgaaa tcgcccgtta
    28681 acataagaca tccgcgtctc ctcgcgcgca agcgcgcgaa ggcctcccgt ggaatatacc
    28741 gctcctcaag ccacccacgg cagattcgat tgatgtgacg cccgggaacg gattccgcgc
    28801 cctgccagac acaccatccg ccgtttacgc ccgcttcgca gtagaaaggc gatccaggca
    28861 gaatgtacgg cacgaaatgc cgccgcatcg caaggtatga ctcgcgccgg atatccagca
    28921 catcgcaatc gggatgagcc gccacgtagg catccagttc attgccaatc atccggaccg
    28981 acgcaacttg gcgcggatcg cggcacttgc cgtggcactc gtccggatag aatgcgcgga
    29041 gcgatttccg aaatgtttcg taatccgccg cggcaaaagg cctttcccgc gcagcggaaa
    29101 gcgcggcagg cgcatcaagc cccaaggccg caaaaaatgc cgcggccgat cccataaatt
    29161 cccgacgctt gaccttcatc tcctgttcat cccctgcgtt cggttcgtga cagggaggca
    29221 tgcgagtcga gccgttcccg actcgctgct ccctttttcg tcaggcacgc aagatctttg
    29281 cccatccctg tcagcaaccg gcatcctcac ttgaggatga tcgccaatcc caccctgcct
    29341 gccaacagcg tgccatcacc cgtgatgagc gccagacggt tttccggcgg cacggagaga
    29401 ttggtcgccg agccataaaa accgtcctta cacttcaccc agtctccgtt cttcttcagc
    29461 cacaaagagc cgatttgctg cacgactccc gaggcgagct ccagcgtgcc cccttcgaga
    29521 tagaactcgg catccttcgg aaatgcatac gaatgaccga tcaccagctc accgccggaa
    29581 accgtcgcct tcttgcccgc ccaggtcccg gccgtcgcgc ttgtctgcgg ataccgcgtc
    29641 caaacattgt tcaccttctg ccagaaatcc cattggtcac cggccgccgc aaagacgaga
    29701 cgtccggcca cgacagacac ctcgcccgtc gatatgttgg cccgccggaa cgtatttgtg
    29761 cctgcaccgg cccgttcgac cgacgccgcc ccgccgaaga ccggcatgat gcccgtatcg
    29821 gcattcgacg ttccctgaca agggattgcg tccgtcaaaa cgagcttcga cccgctcgcg
    29881 ccgaagatcg tgcccccgtt cgcggccgac acgtcgcctc cacatgccgt cagaacaccg
    29941 tccgtcaaga cgccctcaca gcagcagttc gcgaccgcct gacgccaacc gccgagatcc
    30001 acacgcgcct tgggggagac gatctgaatc ggcacggcat tcgaaaacac attcggcgcc
    30061 gaatagacat gcgtgcagca gatcttgaag ttataatccg tggcgaactt gctgccggaa
    30121 gcccccagga caaaccgggc gtagttgttg agctcgctcg aagctccgta gagaaccgca
    30181 tcgttgtcat tcaggtgcgg cgaggtaaaa cgcacttcag acgcgtcatc gaccagactc
    30241 ggatacaaca accgttgggt gatcagcatg aaatcaccgg caaagtccag ccgcgccttc
    30301 gactgaacag taaatgtgaa aaaaccgttc tgcaggctga ccagaccggc cagaacgttc
    30361 gttgtgcttg ccagcgcata aagttggttg tgcccatccg gattgatgcg tgacacggga
    30421 cggttcacaa cagcgttgct aaggtagaga gccgtctcct tgtgcttttc ggcctccgga
    30481 tagatataca gcgcaccggt tccgcccagc ggatcgaggc ccgtgaggta cgtatggccc
    30541 gtgcgaaccg taacgtcgcc ataaatctta ttggcgttcc cggtaatccg cagttcgcca
    30601 taccccgtct tctcgaagga cgccccgccg gccgaatcga acgacgattc aaacgaaagc
    30661 gaactcccct tcggcaccga ggcggcgaga tcgccaccga atttgaaagg cgacttgaag
    30721 acaatcttct tgacgcggtc ggacgtcccc ggacggatgt cgataactcc gttggccagc
    30781 atcagccgac cgttcgcttc cggcgtcgac gtgaattcga ccgtcccgga cgcatcttca
    30841 tcgaaaacaa gcgacttggc gttcacggga acatccagct caacggtcgc attcgacggg
    30901 aaggaaaaga cattgcccgt atagccgaag tccggaagct cggtggcgtt cttccagttc
    30961 ccgagcgtcg tcaccttatc gtcaacacct ccgccgcacc atgtccacgc ctgttccggc
    31021 ggatcgtccg gcggagcgac gaagatcacg cccgcgccag aaaggccttc gcaggccttt
    31081 atctcccccg aggcagtggc ggcataccac ccgaatgcca gagacgtccc cgtggcatcc
    31141 cgcaactcct tgaccgaata ggaaccctcg gcgagttcaa gcgtgccgcc ttccagaaaa
    31201 atcctggcca cgccattgga gaaagcgtcc gccgagcagg tcttgaggac ggcaccgttt
    31261 ccaacgcgca ccgtgccgac ccactgcgcc ccgttcgtca agaccgtcgt tcccgacgtc
    31321 gcgatcagct cgccggaaga agtgcttttc gccagcagtg tgccggtgat acccgacgcc
    31381 agcttcatct caagcgacgc tgtaccttcg aaaacattcc ggttgatgaa atccacaccg
    31441 tcatttacgg taatgagacc gccgacggag ctcgtcacac gacccagccg cgtcacgtca
    31501 ttccacttgt cccaggaggg atccgtgtag gtcgtcagac ggcgaacgct ctgcgtcgtg
    31561 ccattgagat ccaatttact gtatcccggg taaaaagtat tatcagtggc ttcgaaacac
    31621 aaagcggaac tctccgtaat cgccccgttc acgtcacagc ggagggtgaa ggcaatacgc
    31681 gaccagggat accccagcgg cgcatacggt ccagacgtgc atctgttcac cgtattgctg
    31741 acactcgcca gaatcacctt ccccttgtgt gtatacacgt acggcaggag gacggacggg
    31801 ttgttggtaa agacaagttc ggccccttcc gcgaaagaaa acagcaagag cgaatccaaa
    31861 ttcgacgcgc ccgtgacaaa gagcttgcca ttgatcgtcc cgcggaaatt gccctttttg
    31921 acgatcgtcc ccaccgtcgt gactccgtcg aacgtcaccg tcttctgatc gactagagta
    31981 aattccagtg tgcgaagaga acgaatgcct ttataataaa gttgagtccg gctaactgcg
    32041 gtttggtccg gattgaccat aatccggccc gcatccgcgg cgccgaaggc attgtccacc
    32101 gtcccataga caaatccatg cagaacctcg agattacccg tgaaagcgtt cgtggcgttg
    32161 aaatagacgc tgccgtagtt cgatgcgcta cccttttcgc caacgacaag agggccggtt
    32221 ccggaaatga cgccattgaa gtcgacccgc ttattcgccg cgacgcggat cgtcaccttc
    32281 cccggcaaag aaaaagcact tgatatcacg acatcgccgg tgaagtcggc cagactgtcc
    32341 gcaaccggaa cgcctccgtc ccagttgctg gagtcattcc aatcgcctgc cgctccgccg
    32401 cccgtccagg ttgaaccaca gacagaaaac gtcaccgcca ctgataacgc acagatccat
    32461 tttccaagca tattcagttg catttttctg ctccttcttt tcctaccaga atagaccgcg
    32521 aaaacaacgt acgaaaagcc tctaaaaatc tcaattgaaa aatatgcaac tatcgcttaa
    32581 atatttgcaa cacgggtagt cggtcagtga caggggtgga gtgtgcgaag atcatgcgtt
    32641 tccggtgaaa aggagagcaa agagtcggga gcgcgggcgt ccccgcccgc aacagcgtgc
    32701 cttgcgaaag gctgatgcgc atacttccct tgggaaccaa accgcgcatc gggatatcaa
    32761 tcggcctttc acagagcggg cggggacgcc cgcgctcccg actgcgcata cacaaaccac
    32821 gcactaaact cgcatgcctc ctgtcaccaa cccgattact gtgcgctgga acacgcgatt
    32881 gacaaagcga cagaaaagag aaatttgatc tttgccgtgc tggccttgct gggcatgcag
    32941 aatccgttcg tgccaaaggt tcaaattacc tgtcccttag ttgtctctga atttgctatg
    33001 cttgcatcgc tatgacagat agtaaggcca agagagtcgg ggtccttgtt gaactgaccc
    33061 gcgagttcgg gcgcgacctc tgcaagggga ttaccgattt cgcttgttcc catgaaggct
    33121 ttatcccctt tttcatcacc ccggagacgc ttaaacgcaa atccgaactc aaatcgtatg
    33181 acggattcat cgcccgcgtc atgtccgatg agatggcggc gacccttgcc gcacttaaga
    33241 agccgatcgt cgacatgtac tatgacaagc cgcgcccggg gttcgccatc gtcaagacca
    33301 accactcccg catcgggcgg ctcgcggcag agcacttcct agagcgaaaa ttcaagaatt
    33361 tcgccttctg cggctttgcc ggcggacgtt tttcgaacta ctgttcccgt gccttccagc
    33421 gggcgctggc cgcaaaaggg caccgctgcc acagttatca accggacgcc agagccaagt
    33481 acgaattcga cagcacggtt ctgatcaacg agcaactcaa tcgcgcaccg gatgcgaagg
    33541 cgctggcgaa gtggatcggg ctattgccga aaccggtggc ggtgttctgt ccgaatgacc
    33601 ttcgtgcctg gcagttgctg caggtttgca aagaatgcga tatccatgtc cctcatgaca
    33661 ttgcgatcct cggactcgac aacgacgtca tcgtgtgcgg tttttccaac cccatgatat
    33721 cgagcatcga cccagacaca ccagcgatcg ggcgcgaggc cgcgcggaca ttggtcgaga
    33781 tgatgaatga cccggacttc cgcaaaaaga ccctgatccg gcaagtgaac cccaaccgag
    33841 tcatcggacg cgcctccacg gaagtttatc ccattgatcc accttggctt tccgatgccc
    33901 tcgtttacat gcgtaaacgc gttagcgatc aactcaccgc cgatgatgtt ttcaagcatc
    33961 tcggtctctc gcacacgacc gtgaacagga ctttccggaa agtactaaac acaaccgtgc
    34021 agaaggaaat cgccg
//
LOCUS       DAOSLE010000015        30292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1711_length_30292_cov_7.150313, whole genome shotgun sequence.
ACCESSION   DAOSLE010000015 DAOSLE010000000
VERSION     DAOSLE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 30292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30292
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1711_length_30292_cov_7.150313"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2945)
                     /locus_tag="PK576_02250"
     CDS             complement(<1..2945)
                     /locus_tag="PK576_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60443.1"
                     /translation="MKRKRMMVVGAVVFGVAAVRAADVYWWKGSAGDHKWSTADNWAV
                     GSLEGDTATEAPTNGQDVWLVGYAGETIINDVDGVTLNSLHTTNQVDTTHPVMLIGKP
                     LTLSATYKCYTRTTNEVGLVFTTTSAVSIYGANNYNVIRGGISAENAISLTFSNARDL
                     GYDCPIFNVYGAVNVPKGSVRAAQGNSATSFYGPVTAVTLYSGQYYGNGHVHLYAIGN
                     NIGTVYASKYNICNFNAANCFVNNPLLCWDWNNNTDSNGETFRFYGDLTIDHFESTTL
                     QSAEVSNVKRAIGAASGISTVTMKATTNSNTSASYNDKLSLVWDPQGDYTFMNTNRIS
                     TMTGTITVKRGRMVVAGTANFTKLTGITVEDGAAFEAASTSAATTFPLLTTLTLGRNA
                     RFDVTNTTSTVFAQDVTLLVLAEGAKLYIGRDIELKFTGVNRDGVYLASTNFSAATCD
                     WIEGEGTVTTPAVSTLTSWKTPADGDWSTEANWSHGVPAAGKETYLTIQGGTYTARVD
                     VASLVNGRTTLGNTDGNESALLVGNDLAIADGNWLQINAGGAYVQTNGVSEFRNTAEA
                     RRTTVNEGGLWRLEGTATNRFLTSDSGFFTLAGGRIEIADQAELYFENIQHAYNQYSA
                     LDAGRIDISGNGVLKYSSAQNSMANNIRDAEIIVRDNAKFVFTYASFASKANGEFRLV
                     VTNNASYRASACTYVGYNGYSAVPSHCRQIFDFHTDVSPSNYFGWTGFVGVNASGYSE
                     VNIHEGAMVQLGGGYGCRIGACSGKSDKSVCSPTGVVNVAGTARVEGNYYSTYIQQNK
                     AGYNAFYGLLVGGGYQLYYEDIYNNRVHRELPTDSRMKGIVNIAPTGSFLLWQGMLAV
                     GIGTGGDGEINIDGGTFTKARDGNTAIGMGGGKGAVTITGGGRMDAYGTLFIGGVRTN
                     DFESVVYYDDWYVDDGAPASLRDAEGTLRVVNGVYRQAGGAAKIIVGAEGTGMIEIGS
                     NGVVQAEHVV"
     gene            3145..3396
                     /locus_tag="PK576_02255"
     CDS             3145..3396
                     /locus_tag="PK576_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60444.1"
                     /translation="MRMSRFLLLSVVPVLAFGTAAVEVTFIPHRIRTNRTEACGIADF
                     HGDGKLGIVVADMDGNGDWDLVTTGKWGGPVLIENRLKR"
     gene            3393..4451
                     /locus_tag="PK576_02260"
     CDS             3393..4451
                     /locus_tag="PK576_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOE60445.1"
                     /translation="MNTPPLTPYTTTLTDAARRRGIRVTMINPDPELPIFTLSREGRS
                     VRCFNALTDHVGAATYHLLNSKRACHRWLSRHRIPVPTQCVYRDAQPEEALAFLRTHA
                     PVVVKPDSQWGGRGVAMGVRTPAELHAAIRRARRYERDVLLEETVPGEDLRVILAGGK
                     LAAAIRRKPAQVTGDGRHTIRQLITLHNRRRRAEDPSNLIPWDPETRRNLAEQGHAPD
                     DVPAAGCAVQVRLTHNYHTGGTVELATHAVPAYALRLAQRIVRALPVPLIGVDFLIDR
                     ATRRATVVEVSPDMAISPPEGAQVAECFLDVLFPETVTGNATCCRPYPLTAKHGKKTC
                     GTTHAIKMKGSDHASDTP"
     gene            4432..5673
                     /locus_tag="PK576_02265"
     CDS             4432..5673
                     /locus_tag="PK576_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60446.1"
                     /translation="MHLTRHSLSISLLISLLTAHANAEPFPVFSAARAITSGPKDHLF
                     ASYYAINAWSANNRYVTVLETDVKNRLPTEADVAELGVVDLQDNNRFIPLTTTRCWNF
                     QEAAMAHWLATAPDRHFLFNDLVDGRFVTVIFDLQTRTRRIVPFPVSAVSPSGKEAVS
                     INYARLRLTRPDYGYGGKGQDARETVSFPQDDGLFLVDLETGTAKLIVPIASVREQMP
                     SVEEGVDLAYFCHTVFSRDGSKIFWLARSVNPKTARNWKTTSFTCNRDGSGVRRCFPD
                     GWGGSHFNWLDGDRLMVTANYQKKVYTHVLFTVGKTDYTRVGRGMLDFDGHGVFSDDG
                     LWMATDTYPDRLNERKLMVLRMADHAILPLGGFFVPESYRGTYWRCDLHPRWRPDGGM
                     LAFNSVHEGSRQVYVMDVKKR"
     gene            5678..6187
                     /locus_tag="PK576_02270"
     CDS             5678..6187
                     /locus_tag="PK576_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60447.1"
                     /translation="MRRHAWAAAALVIVCGCQHPMENPYFTVTESGLNWVDIRHYRIG
                     NPTQRVWVRLDGNGIVTVREGSSPLVSNPFAKDMTHQQWYDIRETRLTIPRHEAVFLF
                     QTLVDKGLFEKPVKPKNIEEADQIYVSANIQNKTVSYVDPIGDPDLAEQCRLLVMMFH
                     HPKPRARKP"
     gene            6184..7854
                     /locus_tag="PK576_02275"
     CDS             6184..7854
                     /locus_tag="PK576_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60448.1"
                     /translation="MNTKRAKFIVRFPSALRMAGLSLAVLLPCVSRAERLFVGILETD
                     SYQSVIYGASAFSRLADLPLALEWVQGALDRATCLPSFTGVDRNRPLRIVQTVETQKP
                     VGAGNPANVAIIPLTDDGRTVLDALAAAYRKQTRVEPFTRFEEPASTNLTPVVTVVVV
                     KRHLFTSSSAEAVLWAWENRSLLLDAPPQSIPGTLRVLVNPQRLADLTGVRSDKAGAF
                     VNTDRFLRECETFSFALTFESQALTLTVRGRPTAGTGLARLVAAMRPPRATLWHGVPD
                     RAFFASVSACDAPQLWDPYLGSQRLSLVRPYAGIAPTNGFSGDCLISLSPTRDQRGIC
                     LVQIEPVVQTADAEKAIRSLHTVSHADGITLKPLPARRAATGVEIVSYRLAFHAPEAE
                     AEGKTDVSILNTLLELFLKQAVLETAVTEGFVITTVGPVQTLDGELTELTFRERQLTL
                     ERRLRMQDPALADTPLTSGANLRLASLMRHLVSIIPNVKPEHLRPFPTGGDGAALGLG
                     RGANGAFTASLRFGTAELAALQRINRDGRAVLQELFFQMFSSQMMGTQ"
     gene            complement(7913..8977)
                     /locus_tag="PK576_02280"
     CDS             complement(7913..8977)
                     /locus_tag="PK576_02280"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOE60449.1"
                     /translation="MKPKTFVVACGGTGGHVFPGLAVAQELRLRGHAVDVWFSGRAVE
                     RSTLCDWDGPVFLTGARQLSVRTLPQTLNAFFRCARELKRRRPDALLAMGSYTSLPPV
                     VAARLANVPILLHEANAVPGKAVEVLSGMAELTAISFEETAAWMPRRKTVFTGLPVRQ
                     ELIGKPRFDEIPEGMFTVLVTGGSQGARRVNRLVTQAFCLLQQSGSGEFAVLHQSGAA
                     DEARVRQTYEEAGVWARVRAFEPEMGRAYASADLVVCRAGASTCFELCLLGKPALMIP
                     LPSAVRNHQFLNASALARSGGVDVGIEKELSARSIMRYVQNKMRTPACLMHMGEALKA
                     LAHPAAAQRVADALESLSEA"
     gene            complement(8974..10608)
                     /gene="murJ"
                     /locus_tag="PK576_02285"
     CDS             complement(8974..10608)
                     /gene="murJ"
                     /locus_tag="PK576_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOE60450.1"
                     /translation="MSVSQTEQKGPHPRVLRSVSVVSLMTAVSRVLGLVREVTMAYFF
                     GTSALKSAFDIAFIVPNLFRRLFGEGALSSAFVPVFGETLIREGHASAFRFGMRVISM
                     LTVVLGFITTAGILLSFPLARMLPPESRWLIPLPMLRVMLPYALLICIAALLSGMLNT
                     LGKFAIASLTPFLLNLIWIVTLLVVCPFVFRAPEQQIQTLSWAILLAGLAQILFQLPA
                     LGRHGFHFRLRFRGMFHDPGVKRVLYLMGPASLGIGLIQINVCIDKVLAFWADPAAPA
                     ALEYAERLIYLPLGVFGTAFMTVLLPTFSRQASAGELPAMQATLERSLRNLSVIMAPC
                     SAALFVLALPVILLVYSFRGGRFDETSALLSARALAAYAPGLLVFSFQKAITPAFYGL
                     QDLRTPVRVSLCALVLNITLNVFSVLWLPHGWKHVGIAGSTVLTSLVNAVALVILLRR
                     RTVAPRREAVIPTLLKAVGCSLVMAVGVWRVCTVGMRWADGLGEYVKGAQIGVMALTV
                     LCGVILYGGLMVWVGRGAVWEMVGEFRRRPVKGVRQ"
     gene            complement(10619..11842)
                     /gene="lpxK"
                     /locus_tag="PK576_02290"
     CDS             complement(10619..11842)
                     /gene="lpxK"
                     /locus_tag="PK576_02290"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOE60451.1"
                     /translation="MRKVKIRRIERIEHYLVRLIQQPGDDQGHPWPIRTLLAFLKGLS
                     EVFKIIVSLRLFLYEVGICRRYPLGCQVISVGNITAGGTGKTPVVEIFARELQRSGRK
                     VAVLSRGYRKKELPWYQRLFREQIEPPRVVSDGKRILLDSEMGGDEPYMLASNLPGVV
                     VLVDRNRVKSGRYAVKRFGCDTLILDDGFQYQKLKHRLEVVLVDKTNPFGNSQLLPRG
                     VLREPVRNLQRADFIFITKSDGDSDALRARLHELNPTAEIIECRHRARYLQHVFTAER
                     LDLAWLKGRTVTSLSGIAVPQSFENSLRTMGARVIHCERYADHHRYHAQEIINAVNRA
                     ADLKADALLTTEKDAVRFPRLEVTPVPVYYLRVDIELMRGAENFHEAVERICFRPPPR
                     ASAARAQSVPVAEKT"
     gene            complement(12024..12098)
                     /locus_tag="PK576_02295"
     tRNA            complement(12024..12098)
                     /locus_tag="PK576_02295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12063..12065),aa:Glu,seq:ttc)
     gene            complement(12278..13642)
                     /locus_tag="PK576_02300"
     CDS             complement(12278..13642)
                     /locus_tag="PK576_02300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE60452.1"
                     /translation="MRTRFRWVWMTVVLSAGMVFGRMFRDGEVVCFLGDSITNGGRYQ
                     NTVWDYYLTRFPGWTIRFVHAGRSGDSAGGSLGRLQEDVMDYKPTSVAIMMGMNDVGR
                     GNYVANPDEKRKAAQQRSLDGYKANMEKVTERILNEANRPHLFLITPSPFDQTVVLER
                     DNNQPGCNDGLGRCAAIVRELASKVNATVVDFHGPMTAFNLEQQKKDPKYTIVGADRV
                     HPGAPGHLMMAWLFLKAQGVPSLVSRVAVDASAGQVTAGENATVTAVAKRDGGVTFTV
                     LAKALPFPTDPAACKALEGLLPILEELSQETLHVTGLAAGEYALVIDGKEVGRYSAAA
                     LATGISLGANTMTPQYQQALNVSRLNARRRDAECQARNLLNTRRWMKNHHKINVDDPA
                     AVQAHYDGMKDKRQYSAVMALNYIKKWPQYEAYRKNAAELEKEMLASRTPAPHVYEVR
                     PVGR"
     gene            complement(13693..14034)
                     /locus_tag="PK576_02305"
     CDS             complement(13693..14034)
                     /locus_tag="PK576_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HOE60453.1"
                     /translation="MRNKWRVRGLMLACVLGCVCAAQADTMQEIKARRRARREQIRQL
                     VQTGHAKEGDEGYLVAQRALTADQTALMQAENEDRKAGYTAIAQANGKSVAEVGRQAA
                     VIQKNRRASKQ"
     gene            complement(14054..14227)
                     /locus_tag="PK576_02310"
     CDS             complement(14054..14227)
                     /locus_tag="PK576_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60454.1"
                     /translation="MRPKERRFLSVLLLVVTTGCITGCFWVKTEHEVKPIHITMDVNL
                     KVQKELEDFLCLD"
     gene            complement(14243..17257)
                     /locus_tag="PK576_02315"
     CDS             complement(14243..17257)
                     /locus_tag="PK576_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HOE60455.1"
                     /translation="MDARLTKPEPARRWRRLASVVWCGVAVVLSGLLIVDIWLFRVGI
                     VTHMARMTLDRYGFSDTEFAIAGFDTRGVVLEDIRIGQPQPLLEVDQMRLRFRVGDLL
                     RGRLERLDVEGVRLDMRLDEQRITLPLAELLLPRLATIRRPDRKSARIFQLDAATVRE
                     MRIDLLNRKEVPLTQLVLELSAFAEPHGCTRVWGSLGDAATNLCVRVDGTLEPGTGNL
                     SLRSDMRVATLAPVADWIMQATAGEGRRHRIPVRIEGGLTARGEVTAERWREMRTGNM
                     EVALDRQTVIRFEGEDRFVRFQNLRADLTGAPDEWSLRLHAGVSGFRLDAERAVARES
                     GRLLSLRGNARLQRTGRRQHVTATLESDLPGRSLGQLFSAMLPLVPRLFAEGGTLRVE
                     TDLTAEQGGKWTGQTEFLATAKHSSVRVPAGRAGAESVSVSATVPMEKGKAGALQARV
                     AVEDGFVFKKGFVLRGGAEMRLTAAPPYDTANGTLTGRIAEMRALRKRGVSMSQEGVG
                     FESAVQVSGLVTNPVWHLDLHMPETAVAVTNPVCHASAMMGAQARVQYGAQVLAAEGG
                     VWVRDGAARLTVSGGVQNVVAGLERAALRFDLPAYAVSAPDQAVADVSVEVTNGWLRA
                     DDRLALEGLQMFVPLRGSVRDGITCLAGQRFGWDHWEAEGVRLVADGFGVEADTGQIA
                     GRLGVRAVDSAARVDIGVVVPIARPGQAKATVTFPEASLERDDALAARLRKAVPDLEA
                     SARVAAEAAFRLGGGTPHAVGRVCVRDGQFRKKEMSVSGLALDMPFEFSPTFRTIERP
                     SLTFKQIQAGNIRLDAGRIEFDLTPREVSVGSMEVGWCKGSLRAYSVLFDLKDRKDEF
                     IVYADRIDLGEALMMVMPFQGVMEGVLYGRFPVGIDGNRIRLSTGYLYSLPGQGGRLK
                     LDDAAPMASLLDRAGIKGDVQRPLSKALSDLLFHAIRLELEPKPEGDAVLRILLEGKS
                     NDKDWPAPVSLNLNLHGPIEKLVNVGLDLSRE"
     gene            complement(17303..18352)
                     /locus_tag="PK576_02320"
     CDS             complement(17303..18352)
                     /locus_tag="PK576_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60456.1"
                     /translation="MRVRIRWVSFWVTLAFCGLMGVSAEAKSKEEPPKVSVLMKEAND
                     LMSQAQSAYVDGDAPKAIELYRKALAEIVRVERENPNRVTSAEFAPVRFRKALCETEI
                     DRILIEEINASSRTVAVSDTRALEEKRKARQEAAATNRVPEATIKLSVKRGVGGGDAV
                     DHPEDKLADAVRSGSEPVAAKTDAPVTKSETGATAKLDVKKELSWVRELLSLEKWDEA
                     DQNLRTVLRHEPTSRDARFLMALVQMRRGRADDASVMMDDLLADPVTDESVCLLASAV
                     YAATGSYGKAMAALDQAMRLNPQRPLGYLNMAWLLLEMRPADLAEPEMYYRQAVKLGG
                     ARDRDIERRLGIKSE"
     gene            complement(18380..19183)
                     /locus_tag="PK576_02325"
     CDS             complement(18380..19183)
                     /locus_tag="PK576_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE60457.1"
                     /translation="MDYRAKQDKTGRVRRIRRWVNGLGRSRQCCVCGRTFFRFSTYRG
                     GWQQFSAYLHNVKWIGSDFDHFWCPFCRSHDRERHLFLYGEKLGFWEQMRGTSILHIA
                     PEKHVSRRIEACKPERYVKGDLVPSREGVERLDVSAIPYAAASFDWVLCNHVLEHVPD
                     DVRALSELFRVLKPGGHAILQTPYASGLERTLENQAEINTDAQRLAFYGQEDHVRLYG
                     RDLFDRIRGPGFSLELIRHADCLGDVDAARYGVNPDEPLFLCVKPLPAV"
     gene            complement(19186..20166)
                     /locus_tag="PK576_02330"
     CDS             complement(19186..20166)
                     /locus_tag="PK576_02330"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOE60458.1"
                     /translation="MRATDEILTALRQAAGGEVSSRALCDRLGVSRAAIWKRIEALRG
                     LGYGISASPRRGYRLESVPDLPHAAEVLPLLSTRRIGRDCRYVAETGSTNRDAAVLAQ
                     AGAEEGLVVTAGSQHAGRGRMTRVWFSPAGVNLYFSLVLRPAVEPLRAASLPLVAGLA
                     VAEAVADLAPELQPAVKWPNDILVAGRKLCGILCEMQAEIDCVRHIVLGVGVNVNLER
                     ASLPEEIRSRATSLRMACGRSFSRAAVLAAILNHFEPLYDQWLRKGLEPLITRLDARD
                     ALRGRMITLESGGGQTLRGRADGIQADGALRLVTERGPVTLYSGETHIGT"
     gene            complement(20196..20825)
                     /locus_tag="PK576_02335"
     CDS             complement(20196..20825)
                     /locus_tag="PK576_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE60459.1"
                     /translation="MLKAMNYEVIPVGPYAANCSVLWDEPGEAWVVDPGEDGDRILAF
                     LEKRGLRAGMVVLTHAHFDHISATNRVLAVYPVKVYLHQNDVAMAFSPLNAMPPYRAT
                     SRPATLDSSKEDGETLTCGGLTARLIHTPGHTPGGWCLYFEQEGLLVAGDTLFAGSVG
                     RTDFPGGSMLALQRSLDRLKRLPDATRVICGHGPMTTIGAEKRGNPYLR"
     gene            complement(20865..22997)
                     /locus_tag="PK576_02340"
     CDS             complement(20865..22997)
                     /locus_tag="PK576_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60460.1"
                     /translation="MMKMLRSGLILVAVLVARAERVPVFEEGVPQTMCGGASVTAYGG
                     TWIYAAAPDLLDDVSFQQGNATPREKVAPKPAQALKGNYFGVNTTRMPCLNKNLELLE
                     EAVHLIDGDDQTCWLSEAQPRSDMQPIVVRIDLVKETDVAKIVLRKRPLSPETKRRAT
                     DRQPAPDGCEVGRGLPVEMALALSCDAWRWSDVFTGPLGAASEQETFEVAFTPRRAKQ
                     IRLTASKLRQVEFFLYALSLAEIEVLDTQGRNVALVSRGATIAVNATRHQEMPLNMQQ
                     RALWPVQWDLGAKWIRVGYHDDPINWHRVERVKGKLEIDPVADAAISACKEHGQNVIM
                     CLNFGNRLYSGPEKRSFTQLPEWNWCMPNPPATPEALAAWDRYVIFMCRHFRDRVHSF
                     EIWNEWNISVYWGDTPNTEAYCEIVRRTIPLIREHAPQCKVMLGGTSGYFRGLAHQTP
                     EQVAVREEKDSRFVVWKRFAKDVDGIGYHPFYNPNPGWLLDYSADIRAFRAWLARQGF
                     SGTLHASEWNINGRPAPIDPKDARDVWCGIYTQSEIAKAKEVLQQMVRHAGFGIPSCF
                     CEAYNAFYAQTELSLLKGSLLQDPVAPLQPTAAYYALRNAATQMDGFEPAAFTPDVAG
                     TVPGAVAAFAFADGARRGVAVWYEDDQRADTYRQKPVTLTLPFAAAALHAYDPVNGVR
                     QRLNATVDSGKTIVCDLLIGDAPLFVAE"
     gene            complement(23019..25427)
                     /locus_tag="PK576_02345"
     CDS             complement(23019..25427)
                     /locus_tag="PK576_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60461.1"
                     /translation="MRTWLLSAVWLAVSVARAAPVQIAAVDCTREFPTNLYFACGDVV
                     VTNGPAGRYRETAPTEASRFGYRFTVRHPGRPHLFVVRYPDDKTRCMAISDGTAYDMS
                     VGVFTGAAKIGNHGRDYSGLAQPFSNTMVEQRNIFWPRWTDASIVFANTTPGQPAAVA
                     SFAIFELDEQDISPLDIPKAVFPRRSFGVSFEDPCSHGADFGAATYADWLDRAVRCMK
                     HQGQNRLIYPLVWYHGPLYPSAIDTAHYFEWSVPSPVDRKIYIRWTSHPADWPEMLLT
                     RFDREGFEFIAQICLLRYGSLMERMNTDAAAVAAGADTVCNVWNDGVVQAGAGDWTGE
                     YNVNNFAVQCAIREKGKRWGNAPLQYGEKIGGNMRRGPIFNPAHPEVRAAVLRNVREI
                     AERYARHRSFKGLKIFTYGSSFLAWPSLACGYDDVSIARFEKETGCVVDAPKAGAERF
                     MARYKTLTAPPLRRVWIDWRCRVVADLVRDIQCTLRNVRPDLEFTVQLNRARECGVDP
                     TLWRGIGVPDVVGFGGVSPCAHKIKPDVDMRDISDVNIYNTWIERWGKHTWWRCAPGE
                     PVPLSMAFLLGQPAEGICRMGSSFEPDGFWWPDAQMRITPAFSGGVHYMRHFANAVAT
                     CDAFTLVRGGLTFDRAHADLLRPFARAFRALPRVKFETLAGTACGNAVVRTQKAEGAR
                     WLYLVNREYVPCDVTLRVEHPRDGLENGCDAVRPAAEWKIHLEPYALRSFAFPSECSF
                     AWTATDVPPTFASDLEKRAATALTACADSADARDKAYVAEVRGLLSARDWPVLRCALE
                     RRQF"
     gene            complement(25424..26650)
                     /locus_tag="PK576_02350"
     CDS             complement(25424..26650)
                     /locus_tag="PK576_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE60462.1"
                     /translation="MKTKTFSILISIAAISAAFAVDVRDVTLQQRWPWSRLIDVEFVI
                     DGEAGSNMRYDVALEAKNGSDVLAVPTAAILGNRSALASGVHVLTIDPVAAGFPDTVL
                     TRFNVTVTASNSPAYMIIDLTTGAKTYTYDWSDTTWCNTDEYKSDKMAFKHIPAGTFG
                     MGYIGTLPSSTDQTNHQVRLTQDYWLGVHEVTQTQWNKLMGTWPSYFSNVTDRAYRPV
                     ENITLTQIRGAWNVARFACPDTSFIGILAARSGLTMDIPTEAQFEYAQRAGVTVNYYM
                     GITSMITAQSYIRFRENAGGYNYDSYQKLDLRNLTIAEGGTADVGHYPPNAWGLYDMA
                     GNLWEVVYDKGGYKDTTAQIDPVHVPDGTVLEIHGGCFNSPFGSWVYNTSRSTWNCTA
                     VKWEIGFRVMAEYEVK"
     gene            complement(26706..27386)
                     /locus_tag="PK576_02355"
     CDS             complement(26706..27386)
                     /locus_tag="PK576_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60463.1"
                     /translation="MRIERIMQVCLLSLPLLGAGPLIAAPYASATGRVDAAVSARRWQ
                     AVHDRTTTLRWAYPDGAASATVAITNLSGCEVLRQTFTPPAVTCVWTVCSNEVVTADD
                     YFTVTVSFDVGDALVAGVALLKSSFGGGATVVPEGDRRWGRVGDTALIPYDAAWLDAE
                     TAAFSLARRDGQKSTLEPDCTVGWYGWYAKAWGTGWFDLSLSAAGTEDVLSAAVRRVP
                     PGGIVLIE"
     gene            complement(27783..30209)
                     /locus_tag="PK576_02360"
     CDS             complement(27783..30209)
                     /locus_tag="PK576_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60464.1"
                     /translation="MNKGTALSYVSMLFATLVAFNTFAQRFTIENHALRRTLTTEGGV
                     LRTEEIENKLNKSVTPLSQAPEFRLRFSEGTHRPETAFTLTSADFELATIKQAPRKDS
                     EPLSFTLRNAKHGITIAVAFSVNATDAFLHKQLTVTSGKPITLERIDVDALTLPDAYQ
                     PYTTREITAQAPARWSPGLGQPLYGAKSATFWGVEFPAADNLVNEKLLLSGYLWGRQL
                     QAGVPYKSYRAVMGVADDPAFVSDAFRDYITQRRVRPLRLQTQFNSWFDRGRGVNKEN
                     FKEAVEKIHKELVVERGCAPLKAYVVDDGWQDVHADWSGKVWKVNSKFDADFASTLAV
                     IREAKSTLGLWLSPGCLFGASAQVDKLRGQGFEALDVWMSMAGPKYMQALEDRVLELT
                     KQGVGFFKLDGIFGHLNRRTFELKGDKYGLPIMPQLGIEGFKSDDKRLNDSKYDELKI
                     YYLTAGTERLMEIFKKQAAINPEVYIVISNGAWLSPWWLMYIDSVWMINAGDAAGGSS
                     RTEELVYRDSIYYKIWTEQKTQFPISALFNHEPKKTGSNESKEVFRKYLYMNLARGTG
                     FVELYITPRNLAAYDWDVIAEGLQWVKEVFPTFSRAKMFGGDPGKREVYGYTGWQGGV
                     GYIALHNPSEEPSDFVCRLDRAFGLQQSERPERFIVSSPLEGSTRGLPDKVQYGDTMT
                     IRLEPREIRIINFDVASRDWSALKTLQTRTQADYVPPPPKPQPKPIPVGNHPLLGVWE
                     YQHNGTTYTREFVAEGNCILKQGNNIIWTKPVTSKDKNTLIVDDCYTHRLQPDGTLVI
                     ENHYKAKRKK"
BASE COUNT         6150 a         9873 c         8356 g         5913 t
ORIGIN      
        1 accacatgtt cggcctgcac gacgccgttc gagccgatct cgatcatccc cgtaccctcc
       61 gcgccaacga tgatcttcgc cgcgccgcca gcctgacggt acacgccgtt gaccacccgg
      121 agcgtaccct cggcatcacg cagggatgcc ggagcgccgt cgtcgacgta ccagtcatcg
      181 taatagacca cgctttcgaa gtcgtttgtg cgcacgccgc cgatgaacag cgtcccgtag
      241 gcgtccattc tgccgccccc ggtgatcgtc accgcgccct ttccgccacc catgccgatc
      301 gccgtattac cgtcgcgggc cttggtgaag gtgccgccgt cgatgttgat ctcgccgtcg
      361 ccgccggtgc cgatacccac cgcgagcatt ccctgccaga gaaggaacga tcccgtcggc
      421 gcgatgttga cgataccctt catccgcgaa tcagtcggca gttcccggtg caccctgttg
      481 ttgtagatgt cctcgtaata caactgatag ccaccgccga ccaacagtcc gtagaaggca
      541 ttgtatccag ccttgttctg ctggatgtaa gtcgaatagt aattgccttc cacacgggcc
      601 gtgccggcca cgttgacgac accggtggga ctgcagacgc ttttgtcgct cttgcccgaa
      661 caggcgccaa tccggcaacc gtagccgccg ccgagttgca ccatcgcgcc ctcatggatg
      721 ttgacctcgc tgtagccgga agcgttgacg ccgacaaaac ccgtccagcc gaagtagttg
      781 ctcggcgaaa cgtcggtgtg gaaatcgaag atctgccgac aatgggaggg caccgcggaa
      841 tagccgttgt aaccgacgta ggtgcacgca ctcgccctgt aggaggcgtt gttggtgacg
      901 accagacgga attcgccatt cgccttggac gcaaacgacg cataggtgaa aacgaacttc
      961 gcgttatccc ttacgatgat ttcggcatcg cggatattat tcgccatcga attctgcgcc
     1021 gaactgtact tgagaacgcc attgccggag atgtcgatcc tacccgcgtc caaagccgaa
     1081 tactgattgt aggcatgctg aatgttttcg aaatacagct ccgcttgatc ggcgatttcg
     1141 atcctgccgc ccgcaagcgt gaagaagcca ctgtcactgg tgaggaatcg gttggtcgcc
     1201 gttccctcga gccgccacag gccaccttcg ttcaccgtcg tcctgcgcgc ctccgcagta
     1261 ttacggaact cgctcacgcc attcgtctgc acgtaggcac cgccggcgtt gatctggagc
     1321 cagttgccgt ccgcgattgc gagatcgttc ccgaccagca gcgccgactc atttccgtcc
     1381 gtgttcccaa gcgtggtacg cccattcacg agggacgcga cgtccacccg cgcggtgtac
     1441 gtaccgccct gtatggtgag atacgtctcc ttgcccgccg ccggcacgcc atgagaccag
     1501 ttcgcctcgg ttgaccaatc gccgtccgct ggcgttttcc aactcgtcaa cgtcgatacg
     1561 gccggcgtcg tcactgtgcc ctcaccctcg atccagtcgc aggttgccgc cgaaaaattc
     1621 gtactggcaa gatatacgcc gtcacgattc acccccgtga acttcaactc gatgtccctg
     1681 ccgatgtaaa gcttcgctcc ttcggcgagc accaacaaag tgacgtcttg ggcgaagacg
     1741 gtcgaagtcg tgttggtcac gtcgaagcgc gcgtttcggc cgagcgtaag cgttgtcagc
     1801 agcgggaaag tcgttgccgc cgaagtcgac gccgcttcga acgccgcgcc gtcctcgacc
     1861 gtgataccgg tgagtttagt gaaattcgcc gtacccgcca ccaccattcg gccgcgcttt
     1921 accgtgatcg taccggtcat cgtgctgatg cggttggtgt tcatgaacgt gtagtcgccc
     1981 tggggatccc agacgagcga cagtttgtcg ttgtagctcg ccgaggtgtt gctgttggtc
     2041 gtcgccttca tcgtcacggt cgagatcccg ctcgcagcgc cgatcgcccg cttcacgttc
     2101 gacacctcgg cggactgaag cgtggtgctc tcgaaatgat cgatcgtcaa atcgccataa
     2161 aaccggaagg tctcgccatt gctatccgta ttgttgttcc agtcccagca cagcagggga
     2221 ttgttcacga agcagtttgc ggcgttgaaa ttgcagatgt tgtacttgga tgcgtagacg
     2281 gtgccgatat tgttgccgat cgcgtagaga tggacgtgtc cgttaccata gtactgaccc
     2341 gaataaagcg tgactgcggt caccggtccg tagaagctcg tcgcgctgtt gccctgcgcc
     2401 gcccgcacac tccccttcgg cacgttcacc gcaccataga cgttgaagat cggacagtcg
     2461 tagccgagat cgcgcgcatt gctaaaagtc aacgaaatcg cgttctctgc ggagatgccg
     2521 ccacggatga cgttgtaatt gttggcgcca tagatcgaaa ccgcgctggt cgtggtgaag
     2581 acgagaccga cctcgttggt tgtcctcgtg tagcatttgt aggtggcgct cagcgtcagc
     2641 ggtttgccga tgagcatcac cggatgcgtg gtgtcgacct gattggtcgt gtggagggag
     2701 ttaagcgtga caccgtcaac gtcgttgatg atcgtttcac ccgcataccc gacaagccac
     2761 acgtcctgac cgttggtcgg cgcctcggtc gcggtatcgc cttcaagtga gccaaccgcc
     2821 cagttgtccg ccgtcgacca cttatgatcc ccggctgaac ccttccacca ataaacgtct
     2881 gccgcccgca ccgcagcgac gccgaacaca accgcaccga caaccatcat ccgttttctt
     2941 ttcatttttt actacctatt cgagggggtt gcaaaccact aggtttttgt ccgcgaaaca
     3001 cctgccctat tgctatgaaa gaccatgcgc tttatcggca ttgatgtcaa tggtgaaaat
     3061 cgatccgatt gccctgccca atcttttttt gcaagacaaa ctcccgcatg ccgcgctata
     3121 ctgtttcgca tcaggagaga gttcatgcgc atgtcccgtt ttctgttgct gtccgtggtt
     3181 ccggttcttg cgttcggtac tgccgccgtt gaagtgacgt tcatccccca ccggatcaga
     3241 accaaccgaa ctgaagcgtg cggcatcgcg gatttccatg gtgacggcaa actgggtatc
     3301 gtggtcgccg acatggacgg caatggcgat tgggatctgg tcacgaccgg caagtggggc
     3361 ggcccggtgc tgatcgagaa ccggttgaaa cgatgaatac accccccctc accccgtata
     3421 cgactacatt gaccgatgcc gcccgacgcc gcggcatccg cgtcacgatg attaaccccg
     3481 accccgaact gccgattttc accctcagcc gggagggccg gagcgtacgg tgtttcaacg
     3541 cgctcacgga tcatgtcgga gccgccacct accacttgct caacagcaag cgcgcctgcc
     3601 atcgctggct ttcccgtcac cggatcccgg tgccgacgca atgcgtttac cgggacgcgc
     3661 aacccgaaga agcgctcgcc tttctgcgca cacacgcgcc ggtggtggtc aaacctgaca
     3721 gccaatgggg cgggcgcggt gttgctatgg gggtacgcac ccctgctgaa ctgcatgcgg
     3781 ccatccgtcg cgcacgccgc tatgaacgcg atgtgttgct tgaggaaacc gttccgggtg
     3841 aagatctgcg cgtgattctg gcgggtggca agctggccgc cgcgatccgg cgcaagccgg
     3901 cgcaggtcac gggcgacgga cgccacacga tccgccaact cattacgctt cacaaccgcc
     3961 gacgccgggc cgaagaccct agcaacctca tcccgtggga tccggaaacc cgacgcaatc
     4021 tcgcggaaca gggacatgcg cccgatgacg tgcccgctgc cggatgtgcg gtgcaagtcc
     4081 gactgactca taattatcac actggaggta cggtcgagct cgccacgcac gccgtgcccg
     4141 cgtacgcact gcgtctggcg cagcgcatcg tccgtgctct gccggtgccg ctgatcggcg
     4201 tagattttct gatcgaccgc gccacacgcc gggccaccgt ggtcgaagtc agcccggaca
     4261 tggcgatctc cccgcccgaa ggcgcccaag tcgccgaatg ttttttggat gtcctgtttc
     4321 ctgagactgt aacagggaac gccacatgct gtcgtcctta cccgcttacc gcaaaacacg
     4381 ggaaaaagac gtgcggcacg acacacgcaa tcaaaatgaa aggatcggac catgcatctg
     4441 acacgccata gcctgtccat ttccctcctg atctcgttgc tcaccgctca cgcgaatgcg
     4501 gaaccgttcc cggttttctc cgccgcgcga gccatcacgt cggggcccaa agaccacctg
     4561 ttcgcctcgt attacgcgat taatgcgtgg agcgccaaca accgctacgt caccgtgctc
     4621 gaaaccgatg tgaaaaaccg tctcccgacc gaagcggacg tggcggaact cggtgtggtg
     4681 gacctgcagg ataacaaccg cttcataccc ttgaccacca cacgctgctg gaactttcag
     4741 gaggcggcca tggctcactg gctggcaacc gcacccgatc gccacttcct tttcaatgat
     4801 ctggtcgacg gccggttcgt gacggtcatc tttgatttgc agacccgcac ccgacggatc
     4861 gtgccgttcc ccgtcagcgc ggtctcccca tccggtaaag aggccgtcag catcaattac
     4921 gcgcggctac gcctcacgcg ccccgattac ggatacggcg gcaagggaca ggacgcgcgt
     4981 gaaaccgtct ctttccctca ggacgacggg ctttttctgg tcgacctgga gacgggtacg
     5041 gcgaagctga tcgtgccgat cgcatcagtc cgcgaacaga tgccctccgt ggaagaaggg
     5101 gttgacctcg cctatttctg ccatacggtc ttcagtcggg acggatccaa aatcttttgg
     5161 ctggcgcgtt cggtcaaccc caagaccgca cgcaactgga aaaccacgtc cttcacctgc
     5221 aatcgtgacg gcagtggcgt gcgtcgttgt tttccggacg gctggggcgg gtcgcatttc
     5281 aattggctgg acggcgaccg gctgatggtc accgcgaact atcagaaaaa agtatacacg
     5341 catgtgctct tcacggtcgg caagaccgac tacacacgcg tcggccgcgg catgctggat
     5401 ttcgatggtc acggggtctt ttcggatgac ggcctttgga tggcgacgga cacctacccc
     5461 gaccgcctga acgagcgtaa actcatggtc ttgcgtatgg cggatcacgc cattctgccg
     5521 ttgggtggat tcttcgtgcc ggaatcctac cgcggcacct attggcggtg tgacctgcat
     5581 ccgcgctggc ggccagacgg cggcatgctc gcgttcaact ctgtgcacga gggttcccgt
     5641 caggtttatg tgatggacgt gaaaaaaagg tgatgccatg agacgacatg cctgggctgc
     5701 ggccgcactg gttatcgtgt gcggttgcca acacccgatg gaaaacccct attttacagt
     5761 aaccgaatca ggactgaact gggtggatat ccgccattac cggatcggca atccgaccca
     5821 acgcgtctgg gtgcgtctgg acgggaacgg gatcgtcact gtccgcgaag gaagcagtcc
     5881 gctggtcagc aatccgttcg ccaaggacat gacccaccag caatggtacg atatccgcga
     5941 gacgcgactg accattccgc gacacgaagc cgtattcctg ttccagactt tggtggacaa
     6001 gggactcttt gaaaaaccgg tcaaaccgaa aaacatcgag gaggcggatc agatttacgt
     6061 ctcggccaac atccaaaaca aaacggtcag ctatgtggat ccgatcggtg atccagatct
     6121 tgcagaacaa tgccggctgc tggtgatgat gttccatcac cccaaacccc gggcacgtaa
     6181 accatgaaca cgaaacgtgc taagttcatt gtacggttcc cctccgctct gcggatggcg
     6241 gggttgtccc tcgccgttct tctgccctgc gtcagccggg ctgaacgtct ctttgtcggt
     6301 attctcgaaa cggacagtta ccaaagcgtg atctacggcg cttcggcgtt cagccgcctc
     6361 gcggatttgc cgctggcgtt agagtgggtt caaggtgcgc tggaccgtgc aacctgcctg
     6421 ccgtcgttca cgggtgtgga tcggaatcgc ccgctgcgta tcgtgcaaac ggtagagacc
     6481 caaaagcccg ttggtgcggg aaatccagcc aatgtggcaa tcatcccgtt gacggacgac
     6541 gggcggacag tgcttgacgc attggccgca gcctaccgga aacaaacgcg tgtcgaaccc
     6601 ttcacccggt ttgaagagcc cgcctccact aacctgacgc cggtcgtcac ggtggttgtc
     6661 gtcaaacggc acctgttcac ctcgtcttcg gctgaggccg tgttgtgggc ttgggaaaac
     6721 cgttcgcttc tgttggacgc ccccccccag tcgatccccg gcaccctgcg tgttctggtc
     6781 aatccgcaac ggctggctga cctgacagga gttcgcagtg acaaagccgg agcgttcgtc
     6841 aacacggacc gtttccttcg cgaatgcgag accttctctt tcgcattgac cttcgaaagt
     6901 caggccctca ccctgactgt gcgtggccga ccaacggctg gcaccggact tgcccgcctc
     6961 gtcgccgcga tgcgtccgcc gcgcgcgaca ctctggcacg gcgtgccgga tcgcgcgttt
     7021 ttcgcctccg tatcagcgtg tgacgcgccg cagctctggg atccgtatct cggttcgcaa
     7081 cggctcagtc tggtacgacc ctatgcggga atcgcgccca caaacgggtt cagcggcgat
     7141 tgcctgatct ccctttcccc cactcgggat caacgtggca tctgcctcgt gcagatcgag
     7201 ccggtcgtgc agaccgcaga cgcggagaaa gccatccgtt cactgcacac cgtgagccat
     7261 gctgacggca tcaccctcaa accgcttccc gcgcggcgtg ccgcaaccgg tgtggagatc
     7321 gtatcctatc gtctcgcgtt tcacgcgccc gaagccgaag cggaaggcaa gaccgacgtc
     7381 tcgatcctga acacgttgct cgaactcttt ctaaaacagg ccgtattgga aaccgccgtc
     7441 acggaagggt ttgtgattac aacggttggc cccgtccaaa cgctggacgg ggaactgacg
     7501 gagttgacgt ttcgggaacg gcaactcacg ctggaacgac ggttgcgcat gcaggatccg
     7561 gcactggcgg acacaccgct gacctccggc gcgaatttac gcctggcgag tctgatgcgt
     7621 catctggtga gcattatccc gaatgtgaaa ccggagcatc tccgcccgtt tcccaccggg
     7681 ggggacggtg cggcattggg tctggggcgc ggcgccaacg gtgcgttcac ggcttcgtta
     7741 cgcttcggca ccgcggaatt ggcggcgctg cagcgcatca atcgcgacgg gcgcgctgtg
     7801 ctgcaggaac tcttctttca gatgttctcc agccagatga tgggcacgca gtaaacgtcg
     7861 gctgatcccg cttcgcgcac gtcgaatcct gagccctgat gagcactgcc gctcacgctt
     7921 ccgagaggct ttccagcgca tccgccaccc gttgcgccgc agcgggatgg gccaaggcct
     7981 tcaaggcttc ccccatgtgc atgaggcatg cgggcgtccg cattttattc tgcacatagc
     8041 gcataatcga ccttgcggaa agttccttct cgatccccac atccaccccg ccgctacggg
     8101 cgagcgcaga ggcattcaga aactggtggt tccggactgc ggacggcaga ggaatcatca
     8161 gcgcgggctt gccgagcaga cataactcga aacaggtgga ggctccggcc cggcagacaa
     8221 ccaaatcagc agaagcgtaa gcccgcccca tttccggctc gaacgcccgt actctggccc
     8281 agacccccgc ttcctcatat gtctggcgca cccgagcctc atcggccgcc ccgctctggt
     8341 gcaagacggc aaactctccg gacccggact gctggagtag gcagaacgcc tgtgtgacca
     8401 gacggtttac gcgccgcgcg ccctgactgc cgcctgtgac caacacggtg aacattcctt
     8461 ccgggatctc gtcaaaacgc ggcttgccga tcaattcctg ccgaaccggc aacccggtga
     8521 acaccgtctt gcggcgaggc atccaggcgg cggtctcttc aaaactgatc gccgtgagtt
     8581 ccgccatgcc tgacaggacc tcaaccgcct tgcccggcac cgcgttggct tcatgcaaca
     8641 ggatcggcac attggcgagt cgcgccgcca ccaccggcgg caggctggta taactgccca
     8701 tggcgagcaa ggcatccggc cgacgccgtt tcagttcacg cgcacaccga aagaaggcgt
     8761 tcagtgtctg cggcagcgtc cggacggaaa gttggcgcgc accggtcaga aaaacagggc
     8821 cgtcccaatc gcacagcgtg gaacgttcga ctgcccgtcc cgagaaccag acgtccaccg
     8881 catgaccgcg caaccgcaac tcctgagcca cggccaaacc cggaaacaca tgtcccccgg
     8941 ttccgccgca cgccaccaca aaggtttttg gcttcattga cgtaccccct tcaccggccg
     9001 tcgccggaac tcgcccacca tttcccagac cgcccctctc cccacccaca ccatcagccc
     9061 cccatagagg atcacaccac agaggaccgt cagagccatc acaccgatct gcgctccttt
     9121 cacatactcc cccaacccgt cggcccaacg catgcctacc gtgcaaaccc gccatacccc
     9181 cacagccatc accagtgaac aaccgaccgc cttcagcagc gtgggtatca cggcctccct
     9241 gcgcggggcc accgtgcggc gccgaagcag aatcaccagc gcaaccgcat tcaccaagct
     9301 cgtgagcacc gtcgagcccg cgattccgac atgtttccag ccgtgcggca accacagcac
     9361 gctgaacaca ttgagcgtga tattcagcac cagcgcgcac agactcacac gcaccggcgt
     9421 gcgcaagtcc tgtaacccgt aaaaagcggg cgtgatcgct ttttgaaaac tgaacaccag
     9481 cagccccggc gcatacgctg ccaacgcgcg cgcactcaac aacgcggaag tctcatcaaa
     9541 acgtccgccc cggaacgaat aaaccaaaag gatcaccggc agcgccagaa cgaacaacgc
     9601 cgccgaacag ggggccatga tcaccgacaa attccgtagc gaacgctcca gcgtcgcctg
     9661 catcgctggc aactcgcccg cagaggcctg acgtgaaaaa gtcggcagca gtacggtcat
     9721 gaaagcggtc ccgaacaccc ccaacggcag ataaatgagg cgctcggcat attcgagcgc
     9781 cgctggcgcg gccgggtccg cccagaacgc gagaacctta tcgatacaca cgttgatctg
     9841 gatcaacccg atcccgagcg aggccggccc catcagatac agcacgcgtt tcactcctgg
     9901 atcgtggaac atcccgcgga accgcagacg aaaatggaat ccgtgccggc ccaacgccgg
     9961 caactggaaa aggatctggg ccagtcccgc caacagaatc gcccaactga gcgtttggat
    10021 ctgctgttcc ggagcacgga aaacaaaagg gcaaaccacc agcaacgtca cgatccagat
    10081 caggttcagc aaaaaggggg tcaacgacgc gatcgcaaac ttccccaatg tgttgagcat
    10141 gccggaaagt aaggccgcaa tgcagatcag caacgcatac ggcaacatga cgcgcagcat
    10201 cggcaacgga atcagccaac ggctttccgg cggaagcatc cgcgcgagcg ggaaggagag
    10261 caggatccct gcggtcgtga tgaatcccag cacgacggtc agcatactga tcacccgcat
    10321 cccgaaccgg aaagcggagg cgtgcccttc ccggatcagg gtctcgccaa acaccggcac
    10381 aaaggcactc gaaagcgctc cctccccgaa cagtcgccga aacagattcg gcacgatgaa
    10441 cgcgatgtca aacgcggatt tcaacgcgga ggtgccgaaa aaataggcca tcgtcacctc
    10501 gcgcaccaac ccaagcaccc ggctgacagc tgtcatcagg ctgaccacgg aaaccgaacg
    10561 caatacgcgc ggatgcgggc ctttctgctc tgtctgcgat acactcaaca ccggctcctc
    10621 atgtcttttc cgcgaccggc acggactgcg cgcgcgcggc agatgcgcgg gggggaggcc
    10681 gaaaacagat acgctccacc gcctcgtgaa agttttcggc accacgcatc agttcgatgt
    10741 ccacacgcag gtagtagacc ggaaccggcg tgacctccag acgggggaag cggaccgcat
    10801 ccttctccgt ggtcagcaag gcgtcggcct tgagatccgc agcccggttg accgcattaa
    10861 tgatctcctg agcgtgatac cggtggtgat ccgcataccg ttcgcagtgg atcacgcgcg
    10921 cgcccatggt ccgtagcgaa ttttcgaaac tttgcggcac cgcgataccg gacagcgagg
    10981 tcaccgtacg gcccttcagc catgcgagat ccagacgctc cgcagtgaag acatgctgca
    11041 gataccgcgc ccgatgacgg cactcaatga tttccgctgt aggattcaat tcgtggagac
    11101 gcgcacgcaa cgcatcggaa tccccgtccg acttggtgat gaagatgaaa tccgcgcgct
    11161 ggaggttccg gaccggctca cgcaacacac cgcggggcaa gagctggctg ttaccgaacg
    11221 ggttggtctt gtccaccaac accacctcca gacggtgttt gagtttctga tattggaagc
    11281 cgtcatccag aatcagcgtg tcacacccga atcgtttgac ggcataccgt ccgcttttga
    11341 cgcgattccg gtccacaagc accaccacac cgggcaggtt actcgccaac atgtacggct
    11401 catccccgcc catctcgctg tcgagcaaga tccgcttgcc gtcgctgacc acacggggcg
    11461 gttcgatctg ttccctgaac agacgctgat accacggcaa ctctttctta cggtacccgc
    11521 ggctgagtac cgccaccttc cgtcccgagc gctgcaactc gcgcgcgaaa atttccacca
    11581 ccggcgtctt gccggtcccc cccgccgtga tgttcccgac gctgatgact tggcatccca
    11641 agggataacg gcggcagatt cccacctcgt acagaaagag gcgcaacgag acgatgattt
    11701 tgaacacctc agacaggccc ttcaggaatg ccagcaaggt gcggatcggc cacgggtggc
    11761 cttggtcgtc accgggttgt tgaatcagac ggaccagata atgctcgatc cgttcgattc
    11821 gtctgatttt cactttacgc atgggtcacc ccaacatggg aatgggaaaa agaataagat
    11881 atccacgcgc atatgacaat catttgaacc cgttatgcac attccggacc aagccgggcc
    11941 ccaacatgaa attgtagaag gccaaccgcc gcccgactca agcgttctca atccggcgca
    12001 cagggttcga gatatagtaa tgatggcgcg cccacggaga gtcgaactcc gcttccaagg
    12061 atgaaaacct tgtgtcctaa ccgatagacg atgggcgcac aaaaaagtgg ctaaacttta
    12121 tcaaatcggt tcgtacactg gcaagcgtga attccgtttc cccggcggcg cggcaacgcg
    12181 agcacatccc tgattctctg accattgagg gtttcgggtt actgatttca ggagtgacgt
    12241 gatggtctca aacccgaacc cctcaaaagg caacgtgtca ccggccgact ggccgcacct
    12301 catagacgtg cggcgcagga gtccgactgg cgagcatctc cttttcaagt tccgcagcat
    12361 ttttgcggta ggcctcgtat tgcggccatt ttttgatata gttgagagcc atgaccgcgc
    12421 tgtactggcg tttatccttc atcccgtcat agtgcgcctg cacagcagcc ggatcatcca
    12481 cattgatctt gtggtggttt ttcatccacc gccgggtgtt cagcaagtta cgcgcctgac
    12541 actcggcatc ccggcggcgc gcgttcaagc gcgacacatt cagcgcctgc tgatactgcg
    12601 gggtcatcgt gttcgccccc aacgaaatcc cggtcgccag cgccgccgcc gaataccggc
    12661 cgacctcctt gccgtcgatc acgagcgcgt actcgcccgc cgccagaccg gtcacgtgaa
    12721 gcgtctcttg gctgagttct tccaagatcg gcaacagacc ttccaacgcc ttgcatgccg
    12781 caggatccgt cgggaacggc agggccttgg ccaacacggt gaacgtcacg ccgccatccc
    12841 gcttcgcgac cgctgtcacc gtagcgttct cacctgcggt aacctgtccc gccgatgcgt
    12901 caaccgcgac tcgcgaaaca agcgacggca ccccctgggc cttcaaaaac aaccacgcca
    12961 tcatcagatg ccccggcgca ccgggatgca cgcgatccgc tcccacgatc gtgtatttcg
    13021 gatctttttt ctgttgttcg agattgaacg ccgtcatcgg gccgtgaaaa tcaaccaccg
    13081 tcgcattcac tttggacgcc agttcccgaa cgatcgccgc acaacggccc aatccgtcat
    13141 tgcatcccgg ttggttgtta tcccgctcca gcacaaccgt ctggtcaaaa ggcgagggcg
    13201 tgatcagaaa cagatggggc cggttcgctt cgttgaggat tcgctcggtg accttttcca
    13261 tgttggcctt gtatccgtcc agcgagcgct gttgcgcggc cttgcgtttt tcgtccggat
    13321 tcgcgacata attcccccgg cccacatcgt tcatgcccat catgatggcc acggaggtcg
    13381 gtttgtaatc catcacatcc tcctgaagcc gtccaagcga gccgcccgca gagtctcccg
    13441 aacgccccgc gtgcacaaac cggatcgtcc aaccgggaaa acgcgtcaga taatagtccc
    13501 agacggtgtt ctggtaacgc cccccgttcg tgatactgtc ccctaaaaaa cagacgacct
    13561 ccccgtcccg gaacatcctg ccgaaaacca tcccggctga cagcacgacc gtcatccaaa
    13621 cccagcgaaa ccgtgttctc attttccgac tttccttttt ttcgttttcc ttttcttatt
    13681 gcctcatccc cgtcattgtt ttgaggcacg ccggtttttt tggatgaccg cggcctgacg
    13741 ccccacttcc gcgacggact tgccgttcgc ttgggcgatc gcggtatagc cagctttacg
    13801 atcctcgttt tcagcttgca tcaaagccgt ctggtctgct gtcagcgctc tctgagccac
    13861 aagatatcct tcgtccccct ccttggcatg accggtctga accagttgcc ggatttgctc
    13921 acgacgcgct ctgcgccgcg ctttgatctc ctgcatcgta tccgcctgcg ccgcacagac
    13981 gcaaccgagt acgcaggcca gcatcaatcc tctcactctc catttgttac gcatatccgc
    14041 ctccctcttt cgttcagtcc agacagagga aatcttccaa ctctttctgt accttcagat
    14101 tcacatccat cgtgatgtga atcggtttga cctcatgttc ggttttcacc caaaagcatc
    14161 cggtgatgca gccggtcgtc acgaccagca gcagcacgga tagaaaacgc cgttctttgg
    14221 gtctcattct gtcctccttc tttcactccc gggacaagtc cagtcccaca ttcaccaact
    14281 tctcgatcgg cccgtgcaag ttgagattca atgaaaccgg cgcgggccag tccttgtcat
    14341 tcgacttgcc ttccaacaga atccgcaaca ccgcgtcgcc ctccggctta ggctccaact
    14401 ccaaccggat ggcgtggaac aggagatcgc tcaacgcctt ggagagcggc cgctgcacat
    14461 cgcccttgat gcccgcgcga tcgagcagtg acgccatggg agcggcatca tccagtttca
    14521 accgtccgcc ctgacccggc agggagtaaa gatacccggt tgaaagacga atccggtttc
    14581 cgtcaatccc cacggggaac cggccgtaca gcaccccctc catcacgcct tgaaacggca
    14641 tcaccatcat cagggcttcc cccaaatcga tccggtccgc atacacgatg aactcatctt
    14701 tccgatcctt caaatcgaaa agcaccgaat aggcacggag ggagcctttg caccacccca
    14761 cctccatgct gcccacgctc acttcacgcg gtgtgagatc aaattcgatc cgccccgcat
    14821 cgagccggat atttcctgcc tgaatctgtt taaacgtgag ggacggccgt tctattgtgc
    14881 ggaaagtcgg ggaaaattca aaaggcatgt ccaacgccag tccgctcacc gacatttcct
    14941 ttttacgaaa ctggccgtcc cggacacaca cgcgcccgac ggcatgcggt gtgccgccgc
    15001 ccagacgaaa ggccgcctcc gccgcgacac gcgcactcgc ctccagatcg ggtacggctt
    15061 tccgcaggcg ggcggccagc gcatcgtcac gctcaagcga agcctccgga aacgtaacgg
    15121 tcgccttggc ctgtcccggt ctggcgatcg gcacaaccac accgatgtcg acacgtgccg
    15181 cactatcgac cgcgcggaca cccagcctgc cagcgatctg ccccgtgtcc gcttcaacac
    15241 cgaacccgtc ggccaccagc cgcacccctt cggcttccca gtggtcccac ccgaagcgct
    15301 ggcccgccag acaggttatg ccgtcccgta ctgaccctcg taacgggacg aacatctgaa
    15361 gcccttcaag cgccaaccgg tcatctgcgc ggagccatcc gttcgtgacc tcgaccgaca
    15421 cgtcggcgac cgcctgatcc ggtgcggaaa ccgcatacgc tggcaaatca aagcgtaacg
    15481 cagcccgttc gagcccggcc accacgttct gaaccccacc ggagacagtg agcctggctg
    15541 ccccgtcccg tacccacact ccgccttccg ccgcgagaac ttgtgcgccg tactgcacgc
    15601 gcgcttgcgc gcccatcatc gcagaggcgt ggcaaacagg gtttgtaaca gccaccgcag
    15661 tttccggcat atgtaaatcc agatgccaca ccggattcgt taccagcccg ctcacctgaa
    15721 ccgcactctc gaacccgacc ccttcttgcg acatgctgac gccgcgtttc cgcagggccc
    15781 gcatctcggc aatgcgtccg gtcagggtgc cgttcgctgt atcatacggt ggcgcagcgg
    15841 tcagccgcat ctctgccccg ccccgcaaca cgaacccttt tttgaaaaca aacccgtctt
    15901 ccaccgccac acgcgcctgc aatgcgccgg cttttccttt ttccatcggt acggtagcag
    15961 aaaccgaaac ggattcggca cccgcacgac cggcgggcac tctgacggac gagtgctttg
    16021 ccgtagccag aaactcggtc tgtcccgtcc atttcccacc ttgctccgct gtgagatcgg
    16081 tctcgacgcg cagtgtcccg ccttcggcaa acaatcgggg aaccagcggc agcatcgcgg
    16141 agaatagctg tcccagcgaa cgaccgggaa gatctgactc cagcgtggcc gtgacgtgct
    16201 gccgcctccc tgtccgctga agtctcgcgt tccccctgag gctgagcagc cgaccgctct
    16261 cccgcgcgac agcacgctcc gcatccaacc ggaaacccga cacgccggca tgcaaccgca
    16321 gactccactc atccggcgca ccggtgagat cagcccgcag attctgaaac cggacgaaac
    16381 gatcctcccc ctcgaaacgg atcacggtct gccggtccag cgcgacctcc atgttccctg
    16441 tccgcatttc acgccagcgt tccgccgtca cttcgccccg tgcggtcaag ccgccttcga
    16501 tacgtaccgg aatccggtgc ctcctccctt cgccggccgt ggcctgcatg atccagtccg
    16561 cgaccggcgc cagtgtcgca acacgcatat cggagcgcag cgacaggttt ccggttcccg
    16621 gttcgagcgt accgtccaca cgtacacaca agttggttgc ggcatccccg agcgagcccc
    16681 agacgcgcgt gcacccatgt ggttcagcaa aggcactgag ttctaaaacg agttgcgtca
    16741 ggggcacctc tttgcggttc agcaggtcga tccgcatctc gcgcacggtc gcggcgtcga
    16801 gttgaaaaat ccgcgcggac ttgcggtccg gcctgcggat cgttgccaat cgcggcagga
    16861 gaagttccgc cagcggcagc gtgattcttt gttcgtccag acgcatgtcg agacggacgc
    16921 cctcgacatc cagacgctcc aacctgccgc gcaacagatc gcccacgcga aaccgaagcc
    16981 gcatctgatc gacctccagc agcggctgcg gctgaccgat ccggatatct tccagcacca
    17041 caccccgcgt gtcgaacccg gcgatcgcaa actctgtatc cgagaatccg taccggtcca
    17101 gtgtcatacg cgccatgtgc gtcacgattc caacccggaa cagccaaatg tccacgatca
    17161 aaagtccgga caggactacg gccacaccgc accacaccac cgaagccaac cgcctccagc
    17221 ggcgggccgg ttccggtttt gtcagacgtg cgtccatgtc ccccatccgg ccggtcgcgt
    17281 tgcgcccggc atcactcagg cattactcgg atttgattcc cagccgtcgc tcgatgtcac
    17341 ggtcacgcgc gccgccgagt ttcacggcct gccggtaata catctccggc tccgccagat
    17401 ccgcaggccg catttcgagc aacagccaag ccatgttgag ataacctaaa ggccgttgag
    17461 gattgagccg catggcctga tccaacgcgg ccattgcttt gccgtacgat ccggtggcgg
    17521 catataccgc cgaggccagc agacacaccg attcgtcagt caccggatct gccagcagat
    17581 cgtccatcat gaccgacgca tcgtccgccc tgcctcggcg catctgtacc agggccatca
    17641 gaaaacgcgc atcccggcta gtcggttcat gccgcaaaac cgtgcgcagg ttttgatccg
    17701 cctcatccca cttctcaagc gacaacagtt cccgtaccca ggagagttct tttttcacat
    17761 ccagcttcgc ggttgctccg gtctcggact tggtcaccgg cgcgtcggtt tttgcagcaa
    17821 ccggctccga cccggaccgc accgcatccg caagtttgtc ttcgggatga tcaaccgcgt
    17881 caccccctcc cacaccccgt ttgaccgaca gttttatggt ggcttccgga acccggttgg
    17941 tggctgccgc ctcttgccgg gctttccgct tctcctccaa tgcacgggtg tcggagaccg
    18001 cgacggtccg tgaggacgca ttgatctcct cgatcaggat acggtcgatc tcggtttcgc
    18061 acaacgcctt gcggaaacga accggcgcga actcggcgga ggtgacccgg ttcgggtttt
    18121 cacgctccac gcgaacgatc tcagccagcg ccttacggta caactcaatc gcctttgggg
    18181 catcaccgtc gacgtaagcg gactgtgcct gcgacatcag gtcattcgcc tccttcatca
    18241 acaccgaaac tttcggcggc tcctctttgg atttggcttc agccgacacc cccatcaaac
    18301 cgcaaaacgc cagcgtcacc cagaaagaga cccatcgaat ccgcacacgc atagacacct
    18361 cattcatgac ctcagcatat cacacagccg gaaggggctt cacgcacaaa aaaagaggct
    18421 cgtccgggtt gaccccataa cgcgcggcat ccacatcccc gagacaatcc gcatgccgaa
    18481 tcagttccag cgaaaagccc ggtccccgaa tgcggtcaaa gaggtcccgt ccgtaaaggc
    18541 gcacatgatc ttcctgtccg tagaatgcca gacgttgggc atcggtgttg atctccgcct
    18601 gattttcaag tgtccgttcc aaacccgatg cgtagggcgt ctgcaggatc gcatgcccgc
    18661 cgggtttgag cacacggaac agttccgaca acgcccgcac gtcatccggg acatgttcca
    18721 acacatgatt gcacaacacc cagtcgaacg atgcggccgc atacgggatc gcagaaacgt
    18781 cgaggcgctc gaccccctcc cgtgagggca cgagatcccc tttcacataa cgttcgggct
    18841 tgcaggcctc gatgcgtcgg gacacgtgct tttccggcgc gatatgtaaa atcgacgttc
    18901 cgcgcatctg ctcccaaaag cccaacttct ctccgtacag gaacaggtga cgttcacggt
    18961 catgactccg gcagaacggg caccagaagt gatcgaagtc gcttccgatc catttgacgt
    19021 tgtgcagata ggcggaaaat tgttgccatc cccctcgata ggtggagaac cggaagaaag
    19081 tccgtccgca aacacagcat tgacggctgc ggccgagacc gttcacccaa cgccggattc
    19141 tccgcacacg cccggtctta tcctgtttgg cccgataatc catgattagg tcccgatatg
    19201 cgtttcaccc gagtacaagg tcaccggtcc acgctccgtc accagacgca acgccccgtc
    19261 cgcctgaata ccgtccgccc gaccgcgcag cgtctggccg ccgcccgact ccagcgtgat
    19321 catgcggccg cgcaaggcgt cgcgggcatc cagtcgcgtg atcaacggct ccaacccctt
    19381 ccgcagccac tgatcgtaaa gcggttcgaa atggttgagg atcgcggcca gcaccgccgc
    19441 gcgggaaaac gacctgccgc acgccatgcg tagcgaggtc gcgcgggacc ggatctcctc
    19501 aggcaggctg gcacgttcca gattgacgtt cacaccaacc cccaagacga tgtgccgcac
    19561 acaatcgatt tccgcctgca tctcacagag gatgccgcac aacttacgtc cggccaccag
    19621 aatgtcattc ggccacttga ccgcgggttg gagttcgggc gcgagatccg ccaccgcctc
    19681 cgccaccgcc aatcccgcaa ccagcggcag ggacgcggcg cgcaacggct cgaccgcagg
    19741 ccgcagcacc agagaaaaat agaggttcac ccctgcgggc gagaaccaca cacgcgtcat
    19801 ccggccgcgg cccgcgtgtt gcgatccggc cgtcacgacc aacccctcct cggcgccggc
    19861 ttgggccagc accgccgcat cgcgattggt ggacccggtc tccgcgacat atcggcaatc
    19921 ccggccgatc cgccgcgtcg acaaaagggg aagcacctcg gcggcgtgcg gcaggtccgg
    19981 cacactctcc agccgataac cgcgccgtgg cgacgcgctg atcccgtatc cgagtccgcg
    20041 cagcgcctcg atccgtttcc agatcgcggc ccgcgacacg cccagccggt cgcataatgc
    20101 ccggctcgac acctcgccgc cagcggcctg ccgtaaggcc gtcagaatct catccgtcgc
    20161 gcgcatcgtt ctccccgcct cccgttcccg acgcactacc tcaaataagg gttgccccgt
    20221 ttttccgcgc cgatcgtggt catcgggcca tgaccgcaga tcacccgcgt cgcgtccggc
    20281 agtctcttca gccgatccag cgaacgttgt agcgccagca tactgcctcc cggaaaatca
    20341 gttcgtccga ccgatccggc gaacaacgtg tcccctgcca ccagcagacc ttcttgctcg
    20401 aaatacagac accatccgcc gggcgtgtgc ccgggcgtat ggataagccg tgcggtcagt
    20461 ccgccgcagg tcagagtctc gccgtcttct ttggatgaat ccagcgtggc gggacgcgag
    20521 gtcgcccgat aaggcggcat cgcattcaac ggtgaaaacg ccatggccac atcgttctgg
    20581 tgcagataaa ccttcaccgg ataaaccgcc aacacgcgat tcgtcgcgga aatgtggtcg
    20641 aaatgggcat gggtcagcac caccataccc gcacggagac cgcgtttttc cagaaacgca
    20701 agaatccgat ccccatcctc accgggatcc accacccatg cctctcccgg ctcatcccac
    20761 agcacactgc aattcgccgc atacggtccg accggtatca cctcgtaatt catggctttc
    20821 agcatagccg agaaccggac cggagcaaag ccaaaaccaa tggactattc cgcgacgaac
    20881 agcggcgcat cgccgatgag cagatcacac acaatggtct tgccgctgtc cacggtggca
    20941 ttcagacgct ggcgcacacc gttgacggga tcgtaggcat gaagcgcggc tgccgcgaag
    21001 ggcagggtga gcgtgacggg tttttgccga taggtgtccg cccgctggtc gtcctcgtac
    21061 cacacagcca cgccacggcg cgcaccgtct gcgaacgcga acgccgccac ggcaccgggc
    21121 acggttccgg cgacatcggg cgtgaaggcc gccggctcaa agccgtccat ctgcgtggcg
    21181 gcattgcgca acgcgtaata ggcggccgtc ggctggagcg gagccaccgg atcctgcagc
    21241 aacgatcctt tgagaaggct caactcggtt tgtgcgtaga aggcgttgta cgcttcgcag
    21301 aaacaactgg ggatgccgaa gccggcatgg cgcaccatct gctgcagaac ctccttggcc
    21361 ttggcgatct cgctctgcgt gtagatgccg caccagacgt cccgtgcgtc cttcggatcg
    21421 atgggcgcgg gacggccgtt gatgttccat tcgctcgcgt ggagcgtgcc cgaaaagccc
    21481 tggcgcgcca gccaggcgcg gaaggcgcgg atgtctgccg aatagtccag aagccaaccg
    21541 gggttcggat tgtagaacgg gtggtagccg attccgtcca cgtccttcgc gaaacgtttc
    21601 cagacgacaa agcgcgagtc tttttcctcg cgcacggcaa cctgttcggg tgtctgatgc
    21661 gccaaacccc ggaagtaacc gctcgtgccg ccgagcatca ctttgcattg cggcgcgtgt
    21721 tcacggatga gcggaatggt gcggcgcacg atttcgcagt aggcctctgt gttcggggtg
    21781 tcgccccagt acacggagat gttccactcg ttccagattt cgaacgaatg tacgcggtcg
    21841 cggaagtggc ggcacatgaa aatgacgtag cggtcccacg ccgcgagtgc ttcaggcgtc
    21901 gcgggaggat tgggcatgca ccagttccat tccggcagtt gcgtgaacga ccgcttctcc
    21961 gggcccgaat agagccggtt gccgaaattg aggcacatga tgacgttctg gccgtgctcc
    22021 ttgcaggcgc tgatcgcggc gtccgccacc ggatcgattt ccagtttccc tttgacgcgt
    22081 tccacgcgat gccagttgat cggatcgtcg tgatagccta cgcggatcca cttcgcgccc
    22141 agatcccact gcacgggcca gagcgcccgc tgctgcatgt tgagcggcat ttcctgatgc
    22201 cgcgtggcgt tcaccgcgat ggtggcaccg cgtgagacga gcgccacgtt gcgcccctgc
    22261 gtgtcgagca cctcgatttc ggcgagcgac agcgcataca ggaagaactc cacctggcgc
    22321 agtttcgagg ctgtgagccg gatttgcttc gcacgacgcg gcgtgaacgc cacttcgaac
    22381 gtttcctgtt cggacgccgc gccgagaggc cccgtaaaga cgtccgacca acgccatgcg
    22441 tcgcatgaga gggcgagcgc catctccaca gggaggccgc gccccacttc gcaaccatcc
    22501 ggcgcgggct gccggtcggt ggcacggcgt ttcgtttcag gcgaaagcgg acgtttcctg
    22561 aggacgatct tcgccacatc cgtctccttc acgaggtcga tgcgcacgac aatgggctgc
    22621 atgtcgcttc gaggctgcgc ctcgctcagc caacatgttt ggtcatcccc gtcaatgaga
    22681 tgcaccgcct cttcaagaag ttcgaggttt ttgttcagac acggcatgcg cgtcgtgttc
    22741 acgccgaagt aattgccttt aagcgcctgc gcgggcttgg gtgccacctt ctcgcgcggc
    22801 gtagcgttgc cctgctgaaa cgaaacgtcg tcgagcagat cgggcgcggc ggcgtagatc
    22861 cacgtgccgc cataggctgt cacgcttgcg ccgccgcaca tcgtctgtgg caccccctct
    22921 tcaaaaacgg ggacacgctc cgccctcgcc acaagcacag ccaccagaat aagaccagac
    22981 ctaagcatct tcatcattgt cgtgtccttc cttttttatc agaactggcg acgctccaaa
    23041 gcgcatcgca aaacaggcca gtcacgcgct gacaaaagcc cgcgtacctc ggcaacatac
    23101 gctttgtccc gcgcatcggc ggaatccgca catgccgtca acgcggttgc ggcgcgtttt
    23161 tccagatcgc ttgcgaacgt cggcggcacg tccgtagccg tccatgcaaa cgaacactcg
    23221 gacgggaaag cgaaactgcg caacgcatag ggctccagat ggattttcca ttcagcggcg
    23281 ggacggacgg catcacaacc gttctccaac ccgtcgcgcg gatgctccac gcgcagggtc
    23341 acgtcgcacg gcacgtattc acggttcacc agatagagcc accgcgcacc ctcggccttt
    23401 tgcgtgcgca cgaccgcgtt gccgcacgcc gtgcccgcga gtgtttcgaa cttcacgcgc
    23461 ggcagggcgc ggaacgcgcg ggcgaacggg cgcagcagat ccgcgtgcgc ccggtcgaac
    23521 gtgagccccc cgcgcacaag cgtgaacgcg tcgcatgtcg caacggcatt cgcgaaatga
    23581 cgcatatagt ggacaccgcc cgaaaaagcc ggcgtgatgc gcatctgtgc gtcaggccac
    23641 caaaatccgt cgggctcaaa ggacgacccc atccggcata tgccttcggc cggttgtccg
    23701 agaagaaacg ccatgctgag cggcacgggt tcacccggtg cgcaacgcca ccacgtatgc
    23761 ttgccccagc gttcaatcca ggtgttgtag atgttcacat cggagatatc gcgcatatcc
    23821 acgtccggct tgatcttgtg cgcgcaaggg gatacaccgc cgaaaccaac gacatccggc
    23881 acgccgatgc cgcgccagag ggttgggtcc acaccacact cgcgagcccg attaagctgc
    23941 actgtaaact ccagatccgg acggacgttg cggagcgtgc actggatgtc acgcacgaga
    24001 tccgccacca cgcggcaacg ccagtcgatc cacacacgcc gaagcggcgg tgccgtgagc
    24061 gtcttgtaac gcgccatgaa ccgctccgcg cccgccttcg gcgcatccac tacacagcct
    24121 gtttcttttt cgaaccgtgc gatggacacg tcgtcgtagc cgcatgcgag agagggccac
    24181 gcgagaaatg aactgccgta ggtgaagatt tttaaccctt tgaacgaacg gtgccgcgca
    24241 taacgttcgg cgatttcacg tacgttacgc aacacggccg cccgcacctc cgggtgggcc
    24301 ggattgaaga tcgggccgcg ccgcatgttg ccgccgatct tttctccata ttgaaggggc
    24361 gcgtttcccc atcttttccc tttttcccga atggcacact gcaccgcgaa attgttcacg
    24421 ttgtactcgc cggtccagtc gcccgctccg gcctgcacaa cgccatcgtt ccaaacattg
    24481 cagaccgtgt cagcgccggc cgccacggcg gcggcatccg tgttcatgcg ttccatgagg
    24541 cttccatagc ggagcaggca gatttgcgcg atgaattcga acccctcccg gtcgaaacgc
    24601 gtcagcaaca tctcgggcca atccgccgga tgcgacgtcc agcgaatgta gattttacga
    24661 tccaccggcg acggcacgga ccactcaaag taatgcgccg tatcgatcgc cgaaggataa
    24721 agcggcccgt ggtaccagac aagcgggtag atgagccggt tttgtccctg atgcttcatg
    24781 catcgcacgg cacgatcaag ccaatcggca tacgtcgcgg ctccgaaatc ggccccgtgg
    24841 ctgcacgggt cctcaaagct cacgccgaag gagcgacgcg ggaagaccgc tttcgggatg
    24901 tccagcggcg atatgtcctg ctcgtccaac tcgaaaatcg cgaacgacgc gactgcggcg
    24961 ggctgccccg gcgtcgtgtt ggcgaacacg atcgaggcat ccgtccagcg cggccagaaa
    25021 atgttccgct gttcgaccat cgtgttcgaa aacggctggg cgagtccgga ataatctcgg
    25081 ccgtggttac cgatcttcgc ggcacccgtg aacacgccca cactcatatc gtatgccgtg
    25141 ccgtcagaaa tcgccatgca gcgcgtcttg tcgtccggat agcgcaccac gaaaagatgt
    25201 ggacgccccg gatggcgcac cgtgaaacgg taaccgaacc gcgaggcctc cgtcggcgct
    25261 gtctcgcggt aacgcccggc agggccgttc gtcaccacca cgtcgccaca ggcgaaatag
    25321 aggtttgtcg ggaattcgcg cgtgcagtcc acggccgcga tctgcaccgg tgccgcccgt
    25381 gcgacggaaa ccgccagcca gacggcggac agaagccacg ttctcatttc acctcgtact
    25441 ccgccatcac acggaagccg atctcccatt ttaccgccgt acaattccaa gttgaccgcg
    25501 aggtattata cacccaactc ccaaaaggcg aattgaaaca accgccgtgg atctctaaca
    25561 ccgtgccatc gggcacatgg acagggtcga tctgcgccgt cgtgtctttg taaccacctt
    25621 tatcgtagac cacctcccaa agattacccg ccatgtcata taatccccac gcattgggcg
    25681 gataatgacc cacatcggcc gtgccgcctt cggcgatcgt taaattgcgc aggtctaact
    25741 tctggtagga gtcgtaattg taaccaccgg cgttttccct gaaacggatg tacgactggg
    25801 ccgtgatcat agaggtaatt cccatgtagt agttgacggt cacacccgcg cgctgggcat
    25861 attcaaattg agcctcagtg ggaatatcca tcgttaggcc actgcgtgcg gccagaatgc
    25921 cgataaacga cgtgtcggga caggcgaaac gcgccacgtt ccacgcaccg cgtatctgcg
    25981 tcagggtgat gttctcgacg ggacggtagg cacgatctgt gacattcgag aaatatgaag
    26041 gccaagtgcc catcaacttg ttccattggg tttgcgtcac ttcgtggacg cccaaccaat
    26101 agtcctgcgt gagacgcacc tgatggttcg tctgatcagt cgaagaggga agcgtcccta
    26161 tataccccat gccgaacgtg ccggccggaa tgtgcttgaa cgccatcttg tcagatttgt
    26221 actcgtccgt attgcaccac gtggtgtcag accagtcgta ggtataggtt ttagcaccag
    26281 tggtcagatc gatgatcatg taggcgggcg aattcgacgc cgtcaccgtc acgttgaacc
    26341 gggtgagaac agtatccgga aaaccggccg ccacagggtc gatcgtgagg acatgcacac
    26401 cgctcgccaa ggcactcctg ttccccaaga tcgccgctgt cggcacggcc agaacatcgc
    26461 tgccgttttt cgcttcgagc gccacatcgt agcgcatgtt actgcccgct tcgccgtcga
    26521 tgacgaattc gacatcgatg aggcggctcc acggccaccg ttgttgaagc gtgacgtccc
    26581 tgacgtcgac ggcgaacgcc gcgctgatcg cggcaatcga aatgagtata gagaaggttt
    26641 tggttttcat gattatctcc tgatttggct gtgctggaat cgtttgtgga ctttctttcg
    26701 cgttttcact cgatcagaac tatcccgccg ggcggaaccc gccgcacggc ggcggaaagc
    26761 acatcttccg taccagcggc ggaaagcgag aggtcaaacc agcctgtgcc ccaagctttg
    26821 gcgtaccaac cgtaccaccc caccgtgcaa tccggttcga gggtcgactt ctgcccgtcg
    26881 cgccgcgcca gcgagaacgc cgccgtttcc gcgtcgagcc acgcggcgtc ataggggatg
    26941 agcgccgtat cgcccacgcg cccccacctc ctgtcgccct ccggcacgac cgtcgcgccc
    27001 ccaccgaagg aactcttcaa cagggcgacg ccggcaacga gggcgtcgcc gacgtcaaac
    27061 gataccgtca cagtgaaata atcgtccgcc gtcaccacct cattcgagca aaccgtccag
    27121 acacatgtca ccgcaggcgg cgtgaacgtc tgccggagca cctcgcatcc ggaaagattc
    27181 gtgatcgcaa ccgttgcgga cgccgcgccg tcgggatacg cccagcggag cgttgtggtc
    27241 cggtcgtgga cggcctgcca ccgccgcgcg gacacggcag catcaacccg ccccgtcgcg
    27301 gacgcatacg gcgcggcgat caacgggccg gcccccaaca agggcaggga cagcaaacat
    27361 acctgcatga tacgttcgat cctcattgtc tttggcctcc tcatttcggg atgcggcggg
    27421 ccgcattcac gttgctttta tgaaaggaac cggtgcggag cgttcacaag gcgacctccc
    27481 gttctgcttt gacccggacc tgttcttaac attaccacta tacgctcccg acctgtttta
    27541 cggaagtcag atttgcgcaa aaaataatgt caaaaaaacg caaattccgg catttcgcat
    27601 tcgtcggtca ggttaagaat gcgtcatggt cagaggagcg gcgacgtgcc tgtttcgtgc
    27661 aaaactcagc cggcccccaa aatctgcgcc catctgcgtc aatctgcggt tgaaagttca
    27721 gctgcccttt tttctcccgc gcaggcgcga agtttgtgtt catgatggta ttcagaacag
    27781 gtttattttt tacgcttcgc cttgtaatgg ttctcgatca ccagtgtgcc gtcgggctgg
    27841 agccgatgcg tgtaacaatc gtcaacaatg agtgtgtttt tatcttttga cgtgacgggc
    27901 ttggtccaga tgatgttgtt gccttgcttt aagatgcagt tcccttccgc aacaaattcg
    27961 cgtgtgtagg tcgtcccgtt atgttgatat tcccatacgc caagcaacgg atgattcccg
    28021 acagggatgg gcttgggttg aggtttgggc ggtggcggga catagtcggc ttgggtgcgg
    28081 gtctgcaacg ttttcaatgc ggaccagtca cgggacgcca catcaaagtt gatgatgcgg
    28141 atctcacgcg gttccagacg gatcgtcatg gtgtcgccgt actgcacctt gtccggcagg
    28201 ccgcgcgttg agccttcgag aggcgaactc actatgaagc gctctggcct ctcgctctgc
    28261 tgtaagccga acgcacgatc cagacggcag acaaaatcac ttggctcttc agaagggtta
    28321 tggagggcga tatagccgac cccaccctgc cagcccgtat atccatagac ctctctcttg
    28381 ccggggtctc ccccgaacat ctttgccctg gagaatgtgg ggaagacctc cttgacccat
    28441 tgcaagcctt cggcgatgac gtcccagtca tacgcggcga ggttacgggg cgtgatgtag
    28501 agttcgacga atcccgtccc cctcgcgagg ttcatgtaga ggtatttgcg gaagacctct
    28561 tttgactcgt tgctcccggt ctttttaggc tcatggttga agagtgcgct gatggggaac
    28621 tgtgtcttct gttccgtcca aatcttgtaa tagatactgt cgcggtagac gagttcttcg
    28681 gtacgtgaac ttccgcctgc ggcatcgccg gcattgatca tccaaaccga gtcaatatac
    28741 atcagccacc agggactgag ccaggcgccg ttcgagatga cgatgtaaac ctccggattg
    28801 atcgcagcct gcttcttaaa gatctccatc aggcgttcag tgccggcggt gagatagtaa
    28861 atcttgagtt cgtcatactt cgagtcatta agacgtttgt cgtcagactt gaaaccttcg
    28921 atgccgagtt ggggcatgat tggcagcccg tatttgtcgc ccttgagttc aaaggtgcgc
    28981 ctgttcaagt ggccgaagat gccgtccagc ttgaagaaac cgacgccctg tttcgtcagt
    29041 tcgagcacgc ggtcctcaag ggcctgcatg tatttggggc ccgccatgga catccagaca
    29101 tccaacgcct caaaaccttg gcccctcagc ttgtcgacct gtgcgctggc cccgaaaagg
    29161 cagccggggc tcagccagag gccaagtgtg gacttcgcct cgcgaatcac cgccaaggtg
    29221 gaggcgaagt cggcgtcgaa tttactgttc accttccaga ctttgccgga ccagtccgcg
    29281 tggacatcct gccagccgtc gtcgacaaca taagccttta gcggggcgca tccgcgttcc
    29341 accaccagtt ctttgtgaat cttctcgacc gcctctttga aattctcctt gttgacgcca
    29401 cggccacggt cgaaccagga gttgaattgc gtctgcaagc gcagggggcg tacccggcgt
    29461 tgcgtgatat agtcgcggaa ggcgtcgctg acaaaggccg gatcatccgc cacgcccatg
    29521 acggcacgat agctcttgta gggcacgcct gcctggagtt ggcgacccca gagataaccg
    29581 gagaggagga gcttctcgtt gacgaggttg tccgccgccg ggaactcgac accccagaag
    29641 gtcgcgctct ttgcgccata caagggctgg ccaagcccgg gactccagcg ggcgggtgcc
    29701 tgagcggtga tctcccgcgt cgtatagggc tggtaggcgt cggggagggt caacgcatcc
    29761 acgtcgatcc gttccagcgt gatcggcttc ccgctggtca cggtcagttg cttgtgcaaa
    29821 aaggcatctg tcgcattcac ggaaaaggcg accgctatcg taattccatg ctttgcgttc
    29881 cgcagggtaa aagacaaggg ttccgagtcc ttgcgcggcg cctgtttgat cgtggcgagt
    29941 tcaaaatcgg cgctggtgag tgtgaaggcg gtttccggcc ggtgcgtgcc ctcggaaaag
    30001 cgtagacgga actcgggggc ctggctcaag ggagtgacac tcttgttaag cttgttttcg
    30061 atctcctcgg tccggagaac ccctccttct gtagtcagcg ttcgacggag cgcatgattt
    30121 tcaatcgtga agcgctgtgc aaaggtgttg aaagccacta aggtggcgaa gagcatggag
    30181 acgtacgata gcgcggtgcc tttattcatt gtgtgggcat gataacaaac gccaccgctg
    30241 aagaaaaggt cttacaacaa ggaactttcc ccactaccga tttcaacgtt ga
//
LOCUS       DAOSLE010000016        28981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1814_length_28981_cov_9.535055, whole genome shotgun sequence.
ACCESSION   DAOSLE010000016 DAOSLE010000000
VERSION     DAOSLE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28981
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_28981_cov_9.535055"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK576_02365"
     CDS             <1..342
                     /locus_tag="PK576_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE60465.1"
                     /translation="AYVQAAQVLGYSHARIMFRHILPNVTHIILIAFSIRFPASVATE
                     VFISFLGIGVQGEPSWGIMINNARLRLWQGVWWEMTFVTLAIFALVLAFNHLADALRD
                     LLDPALRTEQV"
     gene            390..1391
                     /locus_tag="PK576_02370"
     CDS             390..1391
                     /locus_tag="PK576_02370"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOE60466.1"
                     /translation="MHNVEFGGALPIPSASGQRCVGRWLTGKTKMDDKKTFTALAVLL
                     VGSAAAEVVPPSHAGQVRGCAADSQTYFAAPAPDTFISHRGESHDAPENTLPAFQMAV
                     ERGFGFECDIYLSKDKRVFTFHDGNLRRTTAGACTLACADANWDDVISKCDVGGWGKW
                     EGSKYSPTRPALLEEVLPLARDGRYIYVEVKTGPEIVPYLKEIFAKQTQATPANTLFI
                     SFNQKSCAELKKQMPEYKVFWLTGAHQKGQDGQKVPVAPEDIVSTLKRIGADGVDIYF
                     DPSVVDQAFVRVVRDAGYEFHVWTIDNLDMSLRAFAAGAQTVTTNCAKKQLDAFRAR"
     gene            1543..2334
                     /locus_tag="PK576_02375"
     CDS             1543..2334
                     /locus_tag="PK576_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60467.1"
                     /translation="MGKSIGIMAVIGVCALVCRGQTVQPDERGLWDVWCVGTNSGFVA
                     EESLKACIAFTARTPRDPFVLVMSGFAAWHHLKNGNTEMAVRLFESMQTEKPVTALQK
                     AGDQMARTWLTRLDRERVAHALKKAYLRDIAFPPTLEALKVEGDDAARPPLTDRWGKP
                     WAYRLSSSIQGMAAHRYVLESPGLGADSDLRNALKRPYADGIQLRALRMAAGITDTVE
                     FANPDGVSVRRRLGQEMSGGTLVYLGARLLLLSDGNHWRVLPRPR"
     gene            2403..3689
                     /locus_tag="PK576_02380"
     CDS             2403..3689
                     /locus_tag="PK576_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOE60468.1"
                     /translation="MKLESRKKLVFGRCVPLEETVQRLEAVIGSRYDYRLFETSVSER
                     LHWAALYIDDLEFRSMGKGITPLQAKAGALAECAEWAGAKTHAELQGYMVGHQDVIPD
                     AVGIEEMIPHVTLPEKMIGRVKESDIAMFWADGFSLMTGRTAKLPIEFVRRISGPSGM
                     AAGNYREEAIVHAIEEVFERRVHITVLRQRLVMPTIDPDTVTHPVVREQMAFVRQQGI
                     EVHLKDLSFGGVLPCVGAYFWDPNIPESYQFHHFFKVGAGFNLEEALIRVFTEYAQGR
                     MRDEFIDGNPADQERVLKYDLRALRCIPDDGDNYLSAFLFGFVPQRTANFLREGPVVP
                     FQKGEAFDDCLQDIERAKAIFAQLDRECYVIDFTGPEIGFPVVEVVVPGYSDVLPYYP
                     SDSRVLFRPYTRSDILRSYDAAEGTPSAGGASHKGF"
     gene            3889..4185
                     /locus_tag="PK576_02385"
     CDS             3889..4185
                     /locus_tag="PK576_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60469.1"
                     /translation="MKSEAQKKSIKAGVAMLAATLCMGTVLAGDGSDEPGFVPPPKRS
                     QPPPAPPRSISSAETTDPCCCCPVTPQARTEAKKPPQPPVLITKLKSEGGSSSK"
     gene            4380..5693
                     /locus_tag="PK576_02390"
     CDS             4380..5693
                     /locus_tag="PK576_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOE60470.1"
                     /translation="MKRVRLGWVLAVGAVMAVSGQDEDLDWNATPNDVNNLLKSMKEM
                     IDVNYSVQVATLEEVSVDPAKNPILYRSGHYNFSYTEEQRKRIREYCLAGGMIIYNTG
                     LGSAPFYRSAARELAKIFPEQPLQRLTSDHPIFHAYYDVDRVRYCPGVRKSGFVGDEP
                     WFDAVEINCRVVALVSRWCMAVGWEDNVLPEYQAYEADSAKKLGVNIFSYSSAMRAWA
                     KSQAHAIQFIDKPEPSADKVAMVQVIYDGVWKTRHAGLSVILQTFNLKTDVPVKYTVR
                     EMRLKDPAIFNAPLLYLTGHEHFTLNKSEAANLKRYLENGGFLLAEACCGRKGFDLAF
                     RTLLSALFPGTPLRAIPQDSPIFQVPNDVQRVGVTPVLQQALGSAAIRPQLEGLEVNG
                     HVAVIYSPFGLAGGWEMSQSPYARGYDDVSAQRIGQNILMYSVTN"
     gene            5848..6522
                     /locus_tag="PK576_02395"
     CDS             5848..6522
                     /locus_tag="PK576_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60471.1"
                     /translation="MSRRNVAALVLLTAGVAAAAVRSGNEYALSLVEPKAPAVRKSNA
                     DEGRGKQFFFRYLEVLEVEKGQTGGAPHMTLKTREPSSGVIVRFVVRKSVSLSVLQQA
                     PATVVGDAVAVTGVVSAADPTAREIVLNPVIVRYKDRLAPKVGKEMLSEVDSSAVVYS
                     FTGGGVAVNVSKRDEDLIANEKEMIAKLGKEGWARYLLTEIAKRDKAERAKRDQLDIY
                     RKPQGK"
     gene            6568..7521
                     /locus_tag="PK576_02400"
     CDS             6568..7521
                     /locus_tag="PK576_02400"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOE60472.1"
                     /translation="MAKKGKAKGTVAAKPSRQAGEEGIRAFLNPEEMHKLNRIVLQSR
                     YVVEGNLAGAHRSPLKGASSEFAEHKAYGMGDDPKHLDWKVLGRTDRYYIKRYEDETN
                     LRVYLILDRSGSMSYGSAAVRKFDFACQLVAALGYVVVKARDSVGLFVYSDKLDMQVE
                     PRNSVNHLHNMLKRLQGVEPASTTRTAETLHQIAESIRKRALVVLVSDLLDDEREVTL
                     ALAHFRKRLHDVIVLHVLDPVEIDLSLKQGFEFEDLETGERITADPRGLAKEYQQAFA
                     KFLEHYRVSCEGMRIDYRLVRTGQSVETYARAYLEERRRLR"
     gene            7528..9732
                     /locus_tag="PK576_02405"
     CDS             7528..9732
                     /locus_tag="PK576_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOE60473.1"
                     /translation="MISFLNPLFLAALGALTLPLILHLIQSSRTERLPFSTVRFLKLA
                     QKRSSRRIRMENLLLLILRLLLLALLALAFAMPILRTRNFGSLLSRTSRDVAIVLDGS
                     YSMNYRLNQQSVWNQATELAAAIVEGLGDNDRFCIYLAGDQVTPICEQMTGKKDEATA
                     RLRALPMPVGSSRLCPATLAALGTLNQEGRRGERELHILSDHQMLPWVSFSAGGEGDA
                     TAGAAGSPDPVGDKWDPSAVQDNTTCFVTLLGTPNPENAAVLNVELEPRLITAQTPCQ
                     VTVNLKRTGTPLETAVHLLVDGKETARRSVMLGGEDEARLRFAFPPLEGGVHTAQVTT
                     PDDSLTEDNAFHFLIHVRERLPALCVGSKENALFLRTALSVSADAEAVSSVEVKNISV
                     AELGGENLSAYSCVFLCNVLPLPGQQISLLERYAAAGGLIVLFPGDGAAVSDYAQWNS
                     LPATPAAITEWPLEKRKQLLTWVLPQHPSIRGLVEGGIAPRVVIKRQLQCETLKEKAQ
                     VVVATSAGVPFLINRPAGRGAVVMLTVSADRSWSDFPLSAFYLPLAHQLIQYAAGVGA
                     RPPYLWATDSLSLNEFLPEATRHSTLSAPNGQPVPLRSVMVEGEAVAYAEGLMAPGIY
                     KLSTPGGGGAQPALAINMLREESDLATVKPADISSILGVSALHVATGKEDLMKQLEDF
                     RIGRSLGEALLWLTLLVAVVEVLYANSLLRKTAKLTDTMKIAPSGRVKEKEA"
     gene            9729..11930
                     /locus_tag="PK576_02410"
     CDS             9729..11930
                     /locus_tag="PK576_02410"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOE60474.1"
                     /translation="MIIYDHLAPTAVIAAGVLAALAATGVGYGLFVKRDGPMWVMLLS
                     RVCFFLLLGWCLLLPGEKRVETLEQRTRFLVLVDESRSMTMTPVKGMTNRWQTAQTVL
                     QMPWAAGMAEKCEVEVYPFSADLQQKTSLPELFKREPEGEATLLRDALKKLVGRYAGV
                     DVTGCLLLSDGLDTREAFKEWALEKRPFPIYTLPLEREAIWEEEPDIRVETLSTPRRV
                     TVGWQSELKAVLSGQGTRGQPFAVQLFKDDVLLQRIETQIPEGGGSREVVFKLDHAAI
                     GVNTYRVSLPALSGESQVDDNESSVVVQVLDAKNRLMYVEGPPRWESKYLSRVLRESK
                     QVSPSIFLKGPKGKFMTYGVSGEVAPDMGEAQLAQFKIVVLGNLSGEELGEERARALV
                     KFVEAGGSLVLLGGSKAWAPDGFAKTALKGLLPAKAFSGKAREGEFAVSLTEQGRSHA
                     AFGGDAEFWQKIPAVLSIFQGVVPSAAARVLVEVRSDSGPQPMILAQDFGQGKVVALF
                     SDSLWKWQLSADALKTNPYPRFWHQLLSWLSPKADQIEGRSWEVFLDRETCFLNEEIE
                     VTARWVGEGVPPEGAVVKAEITFPDKRVVPFAVTAREDQMLEGKAVPTFSVKVKGETA
                     GMYAVAAVSESGGQRLESEPAFFAVKPYSPESIPRPVEVSVLRAIAANSGGAYFDSAE
                     ELDRALLSVTPKRIEQSVFEYKTYWQHWAILGILITLIALEWILRKFQNLT"
     gene            11956..12609
                     /locus_tag="PK576_02415"
     CDS             11956..12609
                     /locus_tag="PK576_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60475.1"
                     /translation="MKRIGKGAVWLCGVVLVSALRAAPAETNYCYNSDFTSAKGPLDG
                     WNIDYDWTLIKKQTGNAKNASVLPMFRAKERVLKMAVPSGYETKVETPLIPYEPGDRY
                     QCTFDILVENNLEVHILFLGYNLKPGISPSEPPKLQNLRRVYKAEYVAATAAAWKTMR
                     VTIPNETISPVAYSHLKKVRYITVFMFVPGSTYLRDGNFYVSNLKIVKLPEKVKVTK"
     gene            12654..16256
                     /locus_tag="PK576_02420"
     CDS             12654..16256
                     /locus_tag="PK576_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60476.1"
                     /translation="MAPSVLIGQLEKIGRLLNRWQAEFRLTAGLALFGALVFCLAGVD
                     LLVRYGRNGRLLAWAVLVAGVGVTAGLVFRALARRHTPEAVAVRVEQTFPQLDNHLIN
                     FLLFSAFRVKDSFVAAYVKMDIPHWSGLDFRMMKDRRLLRRAQLALGASLLLLLLPYP
                     FAGRAWPVAMARVLNPFAELTPVSLTRILRVSPGDATVLQGGVLTLACEVQGRAGHTV
                     LLDVRPSDGEQKTYRLGTLRGGAEERFQHVIPKVTTDLKYRFRAGDAYPPGWSTITLR
                     PPLAFRAVTLKVTPPAYMRLPTKRVDAQATALDIPAGSRVELAARCNAPLRSLVVSGW
                     GGDPVEFVEQGKKPGGSVALVVTNGAAFILTAESAEGDKAESTVGFNLLPDRPPALTV
                     RYPQKPVPLPPGMAPTVDFSASDDFGFDEISIVQLPKTGESEARPKVLKTFSAAEIRG
                     KEFSALWKGVVRKTTDTGTWVMRVEARDNREGNGQTTVSGPLVFELDETDQAAKKREE
                     LAKQSRESLERVILLQRENLRKVTQLRDALANSTPEQWAETATRQEEIRQTVRLLLER
                     GGGKSLGNLVPTIKKLYLNELHEVIPTLRSVPATNGITEKDRRVGKALTMQEKILRQL
                     TFAGESAAKAQENQSASSLIGMLDGIITGQAKVLQTASQCATQGVAVAASLVDEQDAL
                     SSEVVAFIQACGAEAAAGQGEDKSQSAFLEGVAAFCKQQKITDDMLLSAEQLEQNAAR
                     EAIPHARSAFDKLTIVRKRFEEVQAQEEKESREEMIEALQIAGAKLEKLKAAEKRLKE
                     EMDKIAENRDRNTEDFDVMEQEAEEIRKNIEDAMLQIPRDLDIFAHLNVGNDLVEDLY
                     SVFEEVTQEKDSGTKDQEGGPVKEKAVMKREHYLEAMEKAEKLIEDVEMWLGDKPDTT
                     KVTDEAFDKEEMPEGVALTPLQTAMEDIIGDLLEIDEKMDREADDGAINQALPDMEMG
                     ALITEGDTTTFSAKGKSGNTTPDHKEQDGRSNVGRQGMSSGESAAGSGTISEGDDNIE
                     ARRTQDPTQSGQVVADGEAQTKATGGGKLGSGKGDDWGDSGGTERMDASEAGSWAQFM
                     EQMARKADQSYAQASLKGLRTDSLKTAAHHIRQASDAVSKGVPIGQVAELRRKAIGAL
                     KKAKTELGEGSATTLDGGVAVSTLTDVIEAEQDLAPAKYRELNADYYKKLNKEL"
     gene            16296..18557
                     /locus_tag="PK576_02425"
     CDS             16296..18557
                     /locus_tag="PK576_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60477.1"
                     /translation="MKTMMRNAIRGLLPAVLAAGLCCRAAEPGEWAVRDATIRFVVGL
                     TRAPSHPSAGFFVKLPDGGSLPGPFPETLAFDEAGNPLTSAVLWHAKEIGCAVVFAAP
                     KEGKTAVLYVRGAKKLATWTPASGLTPSAIRCEVNGTSAKAAAQKLSRLGSVDAKTHF
                     HNQAWSAGTWQGESVPMAVCEWKPGGNAIYLLAHIEVTAPGPTWVAPMIRSGAMEIVI
                     DGKPVPLSKKNEKQGGLGGEVNLTAGLHRVELFGFSPRGQAVGPMMFTWRTSKTTMEE
                     LGGPRASDLRYPGTSMYETAKIKDEHVVKTGDCTLFEIQSQTGLTAAFEAVPDCLFAL
                     EGEEPRVAYTLKAWTKGNPKETRYSWSFEEAPNALADGPTTHWLFKGSRHSRITLTAE
                     AAGRRATCTTTLFAYTFDSSSLDDEETRHAFKRACYSMLKAYPEKADPTAAWDDAMLN
                     NLFRVLDLSGANLLVETIVTQRWPFFKKRLTPERQGLLEDIFLFSLGPRKPQEAIRWA
                     LKLSSEEFSSGRAAVLKLKAAEVMMFYLNDLAGARKLITPLLRDSGEAGEWAKIRMGD
                     LEFLERNINAATQRYGDVQSRAKATAASLTPKRLSDLSATGAARARRSGGKGGESGPA
                     PMAQPADLSAWKLSAIRDAAASEQVNILIEQGFYLEAFQALQAWERIFPLSKLTGDYL
                     LCEAKLYRALKDSKRARVILSAYCDQVDASNFLVAAMELNKQCMMDLNEPAEVIEKYT
                     EEIRRRTFAGGDQ"
     gene            18604..19326
                     /locus_tag="PK576_02430"
     CDS             18604..19326
                     /locus_tag="PK576_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60478.1"
                     /translation="MNGVFVQCVWGVLLACALSVTGAEERSRLYTATDPSATGGLKGR
                     IVRPSQPILQVLAMPPDEPRFVYQGTVTGGDRQEFLFQNLPMARYNLFVIYENEFFEG
                     LELSSEPDTLTPKDRESITRIVNASDPFFNRKVIHRMAGTTGRGNFARCVVTQYRDGP
                     GMVKGDDTALEAGKGRRTFKLIWLKDVGVGWQVVQKRDLYPVTVELKLLTPAHHYSKQ
                     LSRIRVTNRIRDLGELDLRKEK"
     gene            19360..19632
                     /locus_tag="PK576_02435"
                     /pseudo
     CDS             19360..19632
                     /locus_tag="PK576_02435"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
     gene            19735..20715
                     /locus_tag="PK576_02440"
     CDS             19735..20715
                     /locus_tag="PK576_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOE60479.1"
                     /translation="MRESMDAIRREVGKVIIGQEDVLNQILMCIIAGGHALLIGLPGL
                     AKTLTVNTLAKVLDMKFKRIQFTPDLMPSDITGTEVLETDPQTQQKQFRFMQGPIFCN
                     ILLADEINRTPPKTQAALLEAMQEKQVSVGKEIYKLDKPFFVLATQNPIEQEGTYPLP
                     EAQLDRFMFNIFVDYPTYEEEVTVIDRTTMAVGSEPNVVLDKQAVIHLQEVVRKIPVS
                     QHVIRYAVSLVRATRPGDEQAPECTKKYVSTGAGPRAGQFLILAAKARAVLEGRIHVS
                     CNDVRGAAMPVLRHRILTNFSADSEGINSVEIVKRLLEEVKEPGAETYQK"
     gene            20693..21679
                     /locus_tag="PK576_02445"
     CDS             20693..21679
                     /locus_tag="PK576_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOE60480.1"
                     /translation="MRKHTKSRCPQTRAATGQVFETARLPIRVGRLLRMWACVLAGFL
                     PAVAAEKRSPNDAAAPIRGVDALPREQRRAFLRGTSADWRNDTDAARALAPPPIQKAY
                     PADAKRVDLVAPERFTVGDVSAREVIGNRRSRREFSAASFSLEELSYLLWATQGIARI
                     DRDGAGQVTAHYRTVPSGGARHPFESYVAVSRVTGLAPGLYRYLPVEHQLLLLREDAD
                     IHDRVMAACYGQTFIGQAAVTFIWSAVPYRTEWKYGVIAQKLIAIEAGHVCQNLYLAV
                     ESLRAGVCSSMGYSQARLDALLGLDGTEEFVVYLASAGKCGEAPDSGGTPEK"
     gene            21698..24196
                     /locus_tag="PK576_02450"
     CDS             21698..24196
                     /locus_tag="PK576_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE60481.1"
                     /translation="MKPSANSFEPFPFQSVEALRASARRLKLDLPLSGETAVLAEPLR
                     FGGRIAPNRFCVQPMEGCDAEPDGAPSVWTRRRYRRWAEGGFGVIWMEAAAVEGRARS
                     NPRQLWITRRNVRVFAEMVAELRQCACTCWGHEIAILLQLAHAGRYCRPEGVPAPVFI
                     LPDPDPAGIPESLAGQTGLSDEELDRLQDATVTAGLLAMEAGFDGVDLKACHGDLHAE
                     LLGALRRPGRYGGSLENRSRFLREVAVRLRSARPEMIVACRLSPAKEGGEERERIRLL
                     ATAGVGLFNIADSGWTAGDRKKRVNPLRRFMRLAERTQAIQQMVPEIPVVAEGFSRFR
                     RFLPQVAAGVLRRGGGSLIGIGRAALADPGLAGTVLRNGEPDLEACCIDCGACVQLVR
                     DGGRSGCVVTDRAVYGEEYARCRAISLERLREEARRCRGCEPAPCRAGCPTRIDVPAF
                     LRAFAKREDAAAYEIIRRSNVLPGLCASLCPVGTLCEGRCVAGTLDGVPVPIHAIQHA
                     VYRYAHANGLTGLRLPEKPSGRKAAVIGGGPAGVACAVTLLERGHQVVLFERASRLGG
                     TPELAIRDSRFTGAHEEAWAVLMPALRAARLTIRFGVEFGREVTPDSLRRKADAVFLA
                     AGVWGERSVGTAEGVVAGLAFLRQIKTRTRQTVPERVILLAGGDSAMDCARVALEAGT
                     RELLIVYDGPLSEMHWHMADAWFRTAGVHFLTLTRPLGYRTGKDGKVTGLRIRKILES
                     DSDGGPVPETLLEAGLVIEAMGLGLEESLVSALPGCAFNAEGLVKTAEGSLRVSSLPG
                     FFAGGGVINGGASVAQCVAEGMRAGIEMDAELRG"
     gene            24416..25159
                     /locus_tag="PK576_02455"
     CDS             24416..25159
                     /locus_tag="PK576_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE60482.1"
                     /translation="MEKGEAQIIKSRMIPYPKQVTITAGPVVKVDRELAVTVTLKDED
                     CGLKKRVSRLFKSYFGVSPKLTFKTDASLVIPQDGYRFKIASGSGLAIEATGEGGVLN
                     ALKTLRQLAEPERGVEKMRCYFLPEMAVEDAAQWPTMQYFHEKGFPVLACPWKNTEGV
                     RSQARLVNEKGMTGMLCTTWNYMHSLDMYRMLYAGAHATWGYDGTGWNASSAFNRHVR
                     QIGWDIPVKRYRDTGVAQWQVPPENALLH"
     gene            25195..26145
                     /locus_tag="PK576_02460"
     CDS             25195..26145
                     /locus_tag="PK576_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE60483.1"
                     /translation="MKTEEVKAMARECGADLVGIAPVGRFAEEPPERNPAAIFPECRS
                     VIVLGRRICRGSLRGVEEGTNFGSTYQCFGYTWVEDHFLSQTSYDLSLRIEEQGFEAV
                     PIFAYNGAGMPKGVPVAPDRPAPNVYIDPRVAVVAAGVGEIGLGGFLLTPEFGTRQRF
                     AFILTDAELEGDAVRAKTLCGDCGACAEACPFGAIHLDQIQTITVQGQEMPVARVESG
                     LCATCPNGAFLAAGRGKEPDRIAAACGRECLVRLEQSGKLGNRFEQPFRKREPWQLDA
                     LKRPLTRDAAGVLRASDVGCGAAGGTGVVAVHATGQIFSK"
     gene            26153..27262
                     /locus_tag="PK576_02465"
     CDS             26153..27262
                     /locus_tag="PK576_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60484.1"
                     /translation="MTLTSQQIKEFAASLGRPCLVGVADIGRFRSAPRQMHPANIFPE
                     CKSVISVVAPFPRGTLRGITEGTYWPNYTYYSYNRLNNLFRPRITYALSCFIEDHGWE
                     AVPLYPAVPERGGMHRPVKPGRPAREINLNVRIAAVAAGLGEIGWSKVFLTRKWGPRV
                     RIGTILTDAELEPDPLIKPGTLCNRCMKCVRTCPGRAIPSPGERPPVRVEIGDQVYEW
                     GDVSMGRCTLTHHGLNRQVSPFLVQDFPTVDFKVPESRVSEELAYRMAYTLATGTWVA
                     SEEFPHGNIVDYYHQILGHVGYFAICGAQGCVRGCMDSLEKRHAIEQSAFKTAVSPRG
                     QWTLRQPEDEESAGIAEGTCAELYRQPDLQAGRWS"
     gene            27321..28853
                     /locus_tag="PK576_02470"
     CDS             27321..28853
                     /locus_tag="PK576_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE60485.1"
                     /translation="MNRWCVGGMLLVCWVAGWVQAEPLEQGEARFLKARLVPAPQSVT
                     LTDGPRVLLNGALRVSIACVTNGEAAVTETRRVFKEWFGHTPKIGLRHADADGLPQKP
                     DAYRIEASGRTLLIEAADSGGVRNALRTLRQLAEPLRGTREVAEYAIPATRIEDAPVM
                     AFRGFHLCWFPENTPVQMERFIRLAAYYKMNYVVIEPWGMFVSGRKPGLAWPEGTMTR
                     KALRHLVKVADSLGVTLIPQFNALGHASGARMSCHKHAVLDLNPELQPLFEPLGWSWC
                     LSNPETIRVIDECVTDLHEAFGRPPFFHAGCDEADDMGTCPACRRTDYPARVRDHLTH
                     LRDLLAARGCRMMIWHDMLLRSGDPRWKGFYANGKEGAERLLEGLPRDIIICDWYYGA
                     PNKEGTWPTLRYFKEKGFGVLACPWENAEGYRGLGKAVVSEGLEGMLSTTWHHLYGRD
                     MMGIYFRAAHAMWGTRPNGGHEYLVFAHHLRQIGWDVPVRTYEDTGTYHTQLPEKTAS
                     PH"
BASE COUNT         5396 a         7910 c        10256 g         5419 t
ORIGIN      
        1 gcctatgtgc aggccgctca ggtcttgggc tatagccacg cgcggatcat gttccgccac
       61 attctgccca acgtgacgca catcatcctg atcgcgttct cgatccgctt tcccgcgtcg
      121 gtcgccaccg aggtcttcat cagtttcctc gggatcggcg tgcagggcga gccttcgtgg
      181 ggtatcatga tcaacaacgc gcggctgcgc ctgtggcagg gcgtgtggtg ggagatgact
      241 tttgtgacgc tcgcgatctt cgccttggtg ctggcgttca accatctggc cgacgccttg
      301 cgcgacctgc tcgacccggc cctgcgtact gaacaggtgt gagcctgcaa cccaatcatg
      361 atgtgatttt aagttcaaaa ggagagacca tgcataatgt cgagttcgga ggtgctttgc
      421 cgatcccgtc agccagcggc cagcgttgcg ttggccgctg gctgacgggg aaaacgaaaa
      481 tggacgataa aaagacattc accgcactgg cggtcttgct cgtagggagt gctgcggcgg
      541 aagttgtccc gccgtcccac gctggtcaag tccggggatg cgcggctgac agtcagacgt
      601 atttcgccgc ccctgccccc gacaccttca tctctcaccg cggcgaatcg cacgatgcgc
      661 ccgaaaacac gctcccggcc tttcagatgg ccgtggaacg cgggttcggc ttcgagtgcg
      721 acatctatct ctcgaaagac aaacgcgtgt tcacgttcca cgacggcaat ctccgccgca
      781 ccacggccgg tgcctgcacg cttgcgtgcg ccgacgcaaa ttgggacgat gtcatctcga
      841 aatgcgacgt gggaggttgg ggaaaatggg agggttcaaa atactcgccc acgcgccccg
      901 cgctgctcga ggaagtcctt ccgcttgccc gtgacggccg ttacatctac gtggaggtga
      961 agacggggcc cgagatcgtg ccgtatctca aagagatttt cgcgaagcag acgcaagcca
     1021 cgcccgcgaa cacgctcttc atcagtttca accagaagag ttgcgccgaa cttaagaagc
     1081 agatgcccga gtacaaagtt ttttggctca cgggtgccca ccagaagggg caggacggac
     1141 agaaggtgcc cgttgcgccc gaagacatcg tctccacgct gaagcgcatc ggggccgatg
     1201 gtgttgacat ttacttcgat ccttccgtgg ttgaccaggc gtttgtcagg gtcgttcggg
     1261 atgcgggtta cgaattccac gtgtggacga tcgacaatct cgacatgtcg cttcgggcgt
     1321 ttgccgccgg tgcgcagacg gtaaccacca actgcgccaa gaagcaactc gacgcgttcc
     1381 gtgcgcgttg atgacgcacg agcttctttt tcagcagtcg ggaacgcggg gagggggagt
     1441 gtttttacag gcaaagagtt ttggggttgc gggctgcgcc tgtgttagca tatagttcag
     1501 atgttcggat ggtgggaagc ggaacgcggc ggagacaaac ggatgggcaa aagcatcggg
     1561 attatggcgg ttatcggagt ctgcgcattg gtttgccggg ggcagaccgt ccaaccggac
     1621 gagcgggggc tttgggacgt gtggtgtgtg ggcacgaaca gcgggtttgt ggcggaagag
     1681 agcctgaagg cctgcatcgc gttcacggct cgcacgccgc gcgatccgtt cgttttggtg
     1741 atgtcgggtt tcgcggcgtg gcatcatctc aagaacggca acacggagat ggcggtccgg
     1801 cttttcgagt cgatgcagac ggagaagccc gtcacggcgc tccaaaaggc cggggatcag
     1861 atggcgcgga cttggctgac acggttggac cgggagcggg tcgcgcacgc gttgaagaag
     1921 gcctatctgc gggacatcgc gttccctccc acgctggagg ccctgaaagt ggagggggat
     1981 gatgcggccc ggccgcctct gaccgaccgt tggggcaaac cgtgggccta tcggctttcg
     2041 agttcgatcc aaggaatggc ggcgcaccgg tatgtgctgg agtcgccggg gttgggcgcg
     2101 gattccgatc tgcggaacgc gctgaagcgt ccgtatgccg acgggataca gttgcgggct
     2161 ctgcggatgg cggccggtat cacggatacg gttgagttcg cgaaccccga tggggtgtct
     2221 gtccggcgca ggctgggtca ggagatgagc ggcgggacgc tcgtgtatct gggcgcccgc
     2281 ctgctgttgt tgtcggacgg gaaccattgg cgtgttctgc ccagaccccg gtgagcgggg
     2341 gccgtgctct cgtgcctttg tgcgagaggg acgcccgcat ttgcgaggac agggagagcc
     2401 gtatgaaatt ggaaagccgc aaaaaactgg tgttcgggcg gtgcgtgcct ctggaagaga
     2461 cggttcagcg tctggaggcc gtgatcggta gtcggtatga ctaccggctg ttcgagacga
     2521 gtgtctccga gcgtctccat tgggcggcgc tgtacatcga cgatctggag ttccgctcca
     2581 tggggaaggg gatcacgccg ttgcaggcca aggccggagc tctggccgag tgcgcggagt
     2641 gggcgggtgc caagacgcat gcggagctgc aggggtatat ggtcgggcat caggacgtga
     2701 tccccgatgc ggtggggatc gaggagatga tcccgcatgt gacgcttccg gagaagatga
     2761 tcgggcgcgt caaagaatcg gacatcgcca tgttctgggc ggacgggttc tcgctgatga
     2821 ccggcaggac agccaaactc ccgatcgagt tcgtgcggcg gatcagcggg cccagcggca
     2881 tggcggcggg caattaccgc gaggaggcga ttgttcacgc gatcgaggaa gttttcgagc
     2941 ggcgggtgca catcacggtt cttcggcagc gtctggttat gcccacgatc gatccggaca
     3001 cggtcacgca tcccgtggtg cgggagcaga tggcgtttgt ccggcagcag gggattgagg
     3061 tccatctcaa ggatttgtct ttcggcggtg tgctgccctg tgtgggggcc tacttttggg
     3121 acccgaacat tccggagagt tatcagttcc atcatttctt caaagtgggc gcggggttca
     3181 atctggagga ggccctgatc cgggtcttca ccgagtatgc gcagggccgc atgcgggacg
     3241 agttcatcga cgggaatccg gcggatcagg agcgggtgct gaaatacgac ctgcgcgccc
     3301 tccgctgtat tccggatgac ggcgacaact atctctcggc cttcctgttc gggtttgtgc
     3361 cgcagcgcac ggcgaatttt ttacgggagg ggccggtcgt gccctttcag aaaggcgagg
     3421 cgtttgacga ctgcctgcag gatattgagc gggcgaaagc gatcttcgcc caactggacc
     3481 gggagtgtta cgtgatcgat ttcacggggc cggaaatcgg gttcccggtg gtggaggtgg
     3541 tggtgcccgg atattcggac gtgctgccgt attatccgtc tgacagccgg gtgctgttcc
     3601 ggccctacac ccgtagcgat atcctgcgtt cctacgacgc ggcagagggg acgccctcgg
     3661 cgggcggggc ctcgcacaag ggtttctgat cgggtgcggt cgccgtgacc cgtgacggcg
     3721 gattcgaggt gatgatgcgg tgagcggaaa tgcgtctttc ggtgccgaca ggagcggggt
     3781 gaaatcagct tacgaaaaat ttgccgggtc gggttgacag tgccgggggg ccgtgttaag
     3841 gtgttgctat acggtttttg gcgtgacgcc tgttgaggag gggacggcat gaaaagcgaa
     3901 gcccagaaga agagtataaa agccggtgtg gccatgcttg ccgcgacgct gtgcatggga
     3961 acggttctgg ccggtgacgg ttcggacgag cctggttttg tgccgccgcc caagcggtca
     4021 cagccgccgc ccgcgccgcc gcggtcgata tcttcggcgg agaccacgga cccgtgctgt
     4081 tgctgcccgg tcacgccgca ggcccggact gaggccaaga aaccgccgca accgcccgtg
     4141 ctgatcacga agctgaagag cgagggcggt tcgagttcaa agtgagtgtc ggttcggagg
     4201 gcggcggcag aggtatgcaa tgaccgcacg ggagattgac acacccgggg ttatgcggct
     4261 tcagtccgtt gcgggatact ggtctggccg tcggcaggat ttgcgacagt gatgtcccat
     4321 cctgttggcg gcctgtccgt tatctggccg gggcgcggcc ggatgaaagg atcagggata
     4381 tgaagcgggt tcggttgggg tgggtgctgg cggtcggggc cgtgatggcg gtttcggggc
     4441 aggacgagga tctggattgg aacgccacgc cgaatgacgt gaacaatctc ctcaagagca
     4501 tgaaggagat gatcgacgtc aactacagcg tgcaggtcgc gacgctggag gaggtgtcgg
     4561 tggatcccgc caagaacccc atcctctacc gttccggaca ttacaatttc tcgtacactg
     4621 aggaacagcg caaacgcatt cgtgagtact gtctcgcggg aggcatgatc atttacaaca
     4681 cggggctcgg gtcggcaccc ttttaccgct ccgcggcgcg ggagttggcg aagatctttc
     4741 cggagcagcc gctccagcgc ctgacgtcgg atcatccgat cttccacgcc tactacgatg
     4801 tggaccgtgt ccggtattgc cccggcgtga ggaagtccgg ctttgtcggc gatgagcctt
     4861 ggttcgacgc ggtggagatc aactgccggg tggtggcgct ggtgtcgcgc tggtgcatgg
     4921 cggtaggctg ggaggacaat gtcctgccgg agtatcaggc ctatgaggcg gattccgcca
     4981 aaaagctcgg cgtgaacatc ttttcctact cttcggcgat gcgcgcctgg gcgaaaagcc
     5041 aggcccacgc catccagttc atcgacaagc cggaaccgag cgcggacaag gtcgccatgg
     5101 tgcaggtgat ttatgacggc gtctggaaaa cgcggcacgc cgggctttcg gtgattctgc
     5161 agaccttcaa tctgaagacc gacgtgccgg tgaaatacac ggtccgggag atgcggctca
     5221 aggatccggc gattttcaac gcgccgctgc tgtatctgac cgggcacgag cacttcacgc
     5281 tgaacaaaag cgaggcggcc aacctgaaga ggtatttgga gaacggcggg ttcttgctgg
     5341 ccgaggcctg ttgcggacgc aaggggttcg acctggcttt ccgcactctt ctgtcggcgc
     5401 tgtttccggg cacgcccctg cgcgcgattc cccaagacag cccgatcttt caggtgccca
     5461 acgatgtgca gcgggtcggg gtgacgccgg ttctgcagca ggctctgggt tcggcggcca
     5521 tccggccgca actcgagggt ctggaggtga acgggcatgt cgcggtgatt tacagcccgt
     5581 tcgggttggc cggggggtgg gagatgtcgc agagcccgta cgccagaggg tatgacgatg
     5641 tcagcgccca gcgcatcgga cagaacatcc tgatgtattc ggtgaccaat taagtctgcc
     5701 gcctgcggag cggaaacaaa cgatgcggta acgtgatcca cagattacac agatggcaca
     5761 gatcgaatcc aaacctgcgg gaatctgcgg atcggacatg aagttgttag gcaacgtaag
     5821 ggaacggagc ttggaatgaa gggtgcggtg agtcggagaa atgtcgcggc actggtgttg
     5881 ctgacggcgg gcgtcgctgc cgcggcggtg cgcagcggta acgagtacgc gctgagtctg
     5941 gtggagccca aggcacccgc cgtgcgtaag agcaatgccg acgagggccg gggcaagcag
     6001 tttttcttcc gttatctgga ggtgctggag gtggagaagg ggcagaccgg gggggctccc
     6061 cacatgacgc tcaagacccg cgagccgagt tccggcgtga tcgtccggtt tgtggtgcgc
     6121 aagagcgtct ccctttcggt gctgcagcag gcccccgcca ctgttgtggg ggacgcggtg
     6181 gcggtgaccg gtgtggtgtc cgccgctgac ccgacggcgc gcgagatcgt gctgaatccg
     6241 gtgatcgtgc ggtataagga ccgtctggcg ccgaaagtcg gtaaggagat gctttccgag
     6301 gtggactcct cggcggtggt gtactcgttc accgggggcg gggtggcggt gaacgtctcc
     6361 aagcgcgacg aggacctgat cgccaacgag aaggagatga tcgcgaagct cggcaaagag
     6421 gggtgggcga ggtatctgct gacggagatc gccaaacgcg acaaggcgga acgggcgaaa
     6481 cgggaccaac tggatattta ccggaagccg caaggcaagt gatacggaca tgcgggcggg
     6541 cgcgtaaagg atcgggtgaa gcggggtatg gcgaaaaaag ggaaagccaa agggacggtg
     6601 gcggcgaaac cctcccggca ggccggggag gaggggatcc gggccttcct caatccggag
     6661 gagatgcaca agctcaaccg gatcgtgctg cagtcccggt atgtggtgga ggggaatctg
     6721 gcgggcgcgc accggagtcc cctgaagggc gccagttccg agttcgccga gcataaggcg
     6781 tacgggatgg gggacgaccc caagcatttg gactggaagg tgctggggag gacggaccgg
     6841 tattacatca agcggtacga ggacgagacc aatctccggg tttatctgat tctggaccgg
     6901 agcggctcca tgtcctacgg tagcgcggcc gtgcggaagt tcgatttcgc ctgtcagttg
     6961 gtcgccgcat tggggtatgt ggtggtcaag gcccgtgatt cggtcgggct gtttgtctat
     7021 tcggacaagc tggacatgca ggtggagccg cggaattcgg tgaaccatct gcacaacatg
     7081 ctgaaacggt tgcagggggt ggagccggcc tccaccacac gcacggcaga gacgttacac
     7141 cagatcgccg aatcgatccg caagcgtgcg ctggtcgtgc tggtctcgga tctgctggat
     7201 gacgagcggg aggtgacctt ggcgctcgcc catttccgga aacggctgca tgacgtgatc
     7261 gtgctgcatg tgctggatcc ggtggagatc gacctttccc tgaagcaggg gttcgaattt
     7321 gaggatctgg agaccgggga gcggatcacc gcggatcccc gcggcttggc aaaggagtat
     7381 cagcaggcat tcgcgaagtt tctggagcat taccgggtgt cctgtgaagg gatgcggatc
     7441 gactaccgtc tggtgaggac gggccagtcg gtggaaacct acgcgcgggc gtatctcgaa
     7501 gagcggcgga ggctgcggta gcccattatg atttcttttc tcaatccact gtttctggcg
     7561 gcgttgggtg ctttaaccct gccgctgatc ctgcatctga tccagtcgag ccggaccgag
     7621 cggttgccgt tcagcacggt ccggtttctg aaactggcgc agaaacgctc gtcgcggcgc
     7681 atccggatgg agaatctgct gctgctgata ctgcgcctgc tgttgctggc cctgttggct
     7741 ctggcgttcg ccatgccgat cctccgcact cggaacttcg gatccctgct gagccgcacg
     7801 tcgcgggatg tggcgatcgt gctggacggc tcgtacagca tgaactatcg gctgaaccaa
     7861 cagagcgtgt ggaaccaggc cacggagttg gcggccgcga tcgtggaggg gctaggagac
     7921 aacgaccggt tctgcatcta tctggcgggc gaccaggtga cgccgatatg cgagcagatg
     7981 accggcaaga aagatgaggc gacagcccgt cttcgggcgc tgccgatgcc ggtgggctcg
     8041 tcacgcctct gtccggcgac gctggcggct ctggggacac tgaatcagga agggcggcgg
     8101 ggcgagcgcg agctgcacat cctttccgat catcagatgc tgccgtgggt cagtttcagt
     8161 gcgggcgggg agggcgacgc gaccgccggg gccgcaggtt cgcctgaccc ggtcggggac
     8221 aagtgggatc cctcggcggt gcaggacaac accacctgtt tcgttacgct gctggggacg
     8281 cccaatccgg aaaacgccgc ggtcctcaac gtggagttgg agccccggct cattaccgcg
     8341 cagacgccct gtcaggtgac ggtgaatctc aaacgcaccg gcacgccgct ggagacagcg
     8401 gtgcatctgt tggtcgacgg caaggagacg gcacggcgct ccgtgatgct gggcggggag
     8461 gacgaggcgc ggcttcggtt cgcgttcccg ccgttagagg gcggcgtgca tacggcgcag
     8521 gtgacgacgc cggatgacag tctcacggag gacaatgcgt tccactttct gattcacgtc
     8581 cgcgagcggt tgccggcgtt atgtgtgggg tccaaagaaa acgcgctttt cctccgcacc
     8641 gcgttgagcg tgagcgcgga cgcagaggcg gtctcctcag tggaggtcaa aaacatatcg
     8701 gtcgcggaat tgggggggga gaacctctcc gcctactcct gcgtgttttt atgtaacgtg
     8761 ctgccgttgc ccggacagca gatcagcctg ctggagcggt acgcggctgc gggcggcctg
     8821 atcgttctgt ttcccggcga cggtgctgcg gtgtcggatt acgcccagtg gaacagcctg
     8881 cccgcaactc ccgcggcgat cacggagtgg ccccttgaaa agcggaaaca attgctgacg
     8941 tgggtgctgc cgcagcatcc gtccatccgg gggctcgttg aaggggggat cgcgccgcgg
     9001 gtggtgatca aacgccagct ccagtgcgag acgctgaagg aaaaggctca ggtggtcgtg
     9061 gcgacttccg caggggtgcc ctttctcatc aaccgccctg cgggacgggg ggccgtggtg
     9121 atgctcactg tgtcggccga ccgctcgtgg agcgactttc ccctctccgc cttttatctg
     9181 ccgctggcgc atcagttgat ccagtacgcc gcaggggtgg gtgcccgtcc gccgtatctt
     9241 tgggctacgg acagcctctc cctgaacgag ttcttgccgg aggccacacg tcattcgacg
     9301 cttagcgcgc cgaacggcca accggttccg ttgcgcagtg tcatggtgga gggcgaggcg
     9361 gtggcgtacg ccgaagggct gatggcaccg gggatctata agctgagcac gccgggcggc
     9421 gggggcgctc aaccggctct ggcgatcaac atgttgcgcg aagagtcgga tctcgccacg
     9481 gtcaaacctg cggacatctc ctccattctg ggcgtgtccg cgctgcatgt ggcgaccggc
     9541 aaagaggacc tgatgaagca gttggaggac ttccgcatcg gccggtcact gggtgaggcg
     9601 ctgttgtggc tgaccctgct ggtggctgtc gtggaggtgc tttacgccaa cagcctcttg
     9661 cggaagaccg cgaagttgac ggacacgatg aagatcgccc cctccggcag agtgaaggaa
     9721 aaagaagcgt gatcatttac gaccatctcg caccgaccgc tgttatcgcg gcaggggtcc
     9781 tggcggcttt agcggccacc ggggtcggct acggtctgtt tgtgaagcgg gacgggccga
     9841 tgtgggtgat gctgttgtca cgggtctgtt tcttcctgct gctggggtgg tgcctgcttc
     9901 tgcccggcga gaagcgtgtc gaaacgttgg agcagagaac ccgctttctg gtgttggtcg
     9961 acgagtcccg gagcatgacg atgaccccgg tcaaggggat gaccaaccgc tggcagacgg
    10021 cgcagacggt gctccagatg ccgtgggcgg ctggtatggc tgaaaaatgc gaggtcgagg
    10081 tctatccgtt ttccgcggat cttcagcaga aaacttcgct gccggaactc ttcaaacgcg
    10141 agcctgaggg ggaggccacg ttgttgcgcg acgcgctgaa gaaactcgtg ggccggtacg
    10201 cgggcgtgga cgtgacggga tgcctgttgc tcagcgacgg gctggacacg cgcgaggcgt
    10261 tcaaggagtg ggcgttggaa aagcggccct tcccgattta taccctgccg ctggaacgag
    10321 aggcgatctg ggaggaggag ccggatatcc gcgtggagac gctcagcaca ccgcggcgtg
    10381 tcacggtcgg gtggcagtcg gaactcaagg ccgtgctgtc gggacaggga acccggggac
    10441 agcccttcgc agtacagctt ttcaaggacg acgtgctgtt gcagcgtatc gagacgcaga
    10501 tccccgaggg cggcgggtcg cgcgaagtgg tgttcaagct ggatcacgcg gccatcggcg
    10561 tcaacaccta tcgggtctcc ctgcccgcgc tgtccggcga gtcgcaggtc gatgacaacg
    10621 aatcgtcggt ggtggtgcag gtgttggatg ccaagaaccg cctgatgtat gtggaaggcc
    10681 cgccgcggtg ggagtccaaa tatctgtccc gcgtgttgcg cgagagcaag caggtcagcc
    10741 cctcgatttt cctgaagggt cccaagggga agttcatgac gtacggggtg agcggggagg
    10801 tcgcgccgga catgggggag gcgcagctcg cgcagtttaa gattgtggtg ttgggtaatc
    10861 tgagcggcga ggaactgggg gaggagcgcg cgcgcgcgct ggtgaagttt gtggaggcgg
    10921 gcgggagtct ggtgctgctt gggggcagca aagcctgggc accggacggt tttgcgaaga
    10981 cggcattgaa aggactgctg cccgccaaag ccttttcagg caaggcgcgg gagggcgagt
    11041 tcgccgtgag cctgaccgag caggggcgtt cgcacgcggc ctttgggggg gatgccgagt
    11101 tctggcagaa aatcccggcg gtgctttcga tctttcaggg ggtcgtgccc tcggccgcgg
    11161 cgcgagtgct ggtggaggtc cgtagcgaca gcggtccgca accgatgatc cttgcccagg
    11221 atttcggaca gggcaaggtg gtggccctgt tctcggattc gttgtggaaa tggcagttga
    11281 gcgctgacgc gctcaagacc aacccctatc cgcgtttttg gcaccagttg ctctcatggc
    11341 tttcgcccaa ggccgaccag atcgaggggc ggagttggga ggtctttctc gaccgcgaga
    11401 cctgtttcct gaacgaggag attgaagtca ccgcccgttg ggtgggcgag ggcgtcccgc
    11461 ccgagggcgc ggtggtgaaa gcggagatca ccttcccgga caagcgggtg gttccgttcg
    11521 ccgtgacggc gagggaggac cagatgcttg agggcaaggc ggtgccgacc ttcagcgtga
    11581 aggttaaagg cgaaaccgcg ggaatgtacg cggtggcggc ggtttcggag tcgggtgggc
    11641 agcggctgga gtccgagccg gcctttttcg cggtgaagcc ttactcgccc gagtccatcc
    11701 cccgtcctgt ggaggtgtcg gtgttgcggg cgatcgcggc gaacagcggc ggcgcgtatt
    11761 tcgattctgc ggaagagttg gatcgcgcgt tgttgtccgt gacgcccaag cgcatcgagc
    11821 agagcgtctt cgagtataag acgtattggc aacattgggc gattcttggt atcctaatca
    11881 ctttgatcgc tctggagtgg attctccgaa aattccagaa tctcacgtag acggtcatgc
    11941 gaccgggagg ggcagatgaa acggatcgga aaaggtgccg tgtggctttg cggggttgtt
    12001 ctggtgtcgg cgttgcgcgc ggcacccgcg gagaccaact attgttacaa cagcgacttc
    12061 acgtccgcca aagggccgct ggacggctgg aatatcgatt atgactggac cctcatcaag
    12121 aaacagacgg gcaatgccaa gaacgcctcg gtcctgccga tgttccgggc gaaggagcgg
    12181 gtgctgaaaa tggcggtgcc gagcggatat gagacgaagg tcgagacgcc gctgatcccg
    12241 tatgagcctg gggaccgcta ccagtgcacc tttgacattc tggtggagaa taatttggag
    12301 gtgcacattt tgtttctggg gtacaacctc aagccgggca tttcgccgag cgaacccccg
    12361 aaactgcaga acctgcggcg cgtgtacaag gcggagtacg tcgcggcgac agcggcggcg
    12421 tggaagacta tgcgcgtcac catccccaac gagacgatct cgccggtcgc gtacagccac
    12481 ctgaaaaaag tccggtacat cacggtcttc atgtttgtgc caggctcgac gtatttgagg
    12541 gacgggaatt tttacgtgtc caacctgaag atcgtcaagt tgccggagaa ggtcaaggta
    12601 acgaaatgag gtcgtgccgc gcctgagcgc ggcctgatgc acggagacgg gatatggctc
    12661 catccgtatt gatcggacag cttgaaaaga tcggccgact gctgaaccgc tggcaggcgg
    12721 agttccggct gacggccggg ctggcgttgt ttggggcgct ggtcttctgt ctggcgggtg
    12781 tcgatctgct ggtccggtac ggccgcaacg ggcggttgct ggcctgggcc gtgctggtgg
    12841 cgggggtcgg cgtgacggcg gggctggtct tccgcgcgtt ggcgcggcgc cacactcccg
    12901 aggccgtggc agtccgtgtg gagcagacct tcccgcaact cgataaccat ctgatcaact
    12961 ttcttctgtt ttccgcgttc cgtgtcaagg actcgtttgt cgcggcgtat gtgaaaatgg
    13021 atatcccgca ctggagcggg ttggatttcc gcatgatgaa ggaccggcgg ctgctgcgcc
    13081 gggcgcaact tgcgctgggc gcgtcgctgt tgctgctgtt gctgccgtat ccgttcgcgg
    13141 ggcgggcgtg gcccgtggcg atggcgcggg tactgaaccc cttcgctgaa ctcacgccgg
    13201 tcagcctgac ccgcattctc cgcgtgagcc ccggcgacgc gaccgtcctg cagggcggtg
    13261 tgcttacgct ggcgtgcgag gtgcagggcc gggccgggca cacggtgctt ctggatgtcc
    13321 gtccatccga cggcgagcag aagacctatc ggttgggtac gctccgggga ggcgcggagg
    13381 agcggttcca gcatgtgatc cccaaagtca ccaccgacct gaaataccgc ttccgcgcgg
    13441 gggacgcgta tccgccggga tggagcacga tcaccctgcg gccgcccttg gcattccgcg
    13501 ccgtgaccct gaaggtgacg ccgcccgcct atatgcgcct gccgacgaaa cgggttgatg
    13561 cgcaggcgac ggccctcgac atcccggccg gatcccgggt tgaactcgcg gcgcgctgta
    13621 acgcgccgtt gcgttcgctg gtcgtttccg gctggggcgg tgatccggtg gagttcgttg
    13681 agcaagggaa gaaaccgggc gggagcgtcg cgctcgtggt cacgaacggt gccgccttta
    13741 tcctcaccgc agagagcgcg gaaggcgaca aggcggagag cacagtgggg ttcaacctcc
    13801 tgccggaccg tccgcccgcg ctaacggttc gatatccgca gaaacccgtg ccgttgccgc
    13861 ccggtatggc tccgacggtg gatttttcgg cctcggatga cttcgggttc gatgagatct
    13921 ccattgtgca gcttcccaaa acaggcgagt ccgaagcgcg gcccaaggtg ctgaagacct
    13981 tctctgctgc ggagatccgg ggcaaagagt tttccgcgct gtggaagggt gtggtccgta
    14041 aaacaacgga caccggcacg tgggtgatgc gggttgaggc ccgggacaac cgcgagggca
    14101 acggccagac cacggtatcc ggtccgctgg tgtttgaact ggacgagacg gatcaggcgg
    14161 cgaagaagcg ggaggaactg gccaaacaga gcagggagag tctggagcgg gtcatcctcc
    14221 ttcaacgtga gaatctccgc aaggtcacgc agcttcgcga tgctttggcg aacagcaccc
    14281 cggaacagtg ggctgagacc gccacgcgac aagaggagat ccgccagacg gtccggctgc
    14341 tgttggagcg gggcggcggc aagagcttgg ggaatttggt gcccacgatc aagaaactct
    14401 acctgaacga actgcatgaa gtgatcccca cactgcggtc ggttccggcc acgaacggga
    14461 taacggaaaa agatcggcgg gttggcaagg ccctgacgat gcaggagaaa atcctccggc
    14521 aactgacctt tgccggggag tccgcggcaa aagcccagga gaaccagagc gccagttccc
    14581 tgatcgggat gctcgacggg atcatcaccg ggcaggcgaa ggtcttgcag accgcttccc
    14641 agtgcgccac tcagggggtg gcggtcgcgg cctcgctggt ggatgagcag gacgcgctga
    14701 gttcggaggt ggtggccttc attcaggcct gcggggccga ggcggcggcg ggacagggcg
    14761 aggacaaatc gcagagcgcg tttctcgaag gggtcgccgc tttttgcaaa cagcaaaaga
    14821 tcacggacga catgctgctg tccgctgaac aactggagca gaacgcggcg cgcgaggcga
    14881 tcccgcacgc gcggagcgcg tttgacaagc tgacgattgt gcgcaaacgg ttcgaggagg
    14941 ttcaggcgca ggaggagaag gagtcgcgcg aagagatgat cgaggcactc cagatcgccg
    15001 gggcgaaact ggagaagctc aaggcggccg aaaaacggtt gaaggaagag atggataaaa
    15061 tcgccgaaaa ccgggaccgg aacacggaag atttcgacgt catggagcag gaggccgagg
    15121 agatccggaa gaacatcgag gatgcgatgc tccagatccc gcgcgatttg gatattttcg
    15181 cccacctgaa tgtcggcaac gatctggtcg aggaccttta ttccgtgttc gaggaggtga
    15241 ctcaggaaaa ggactcgggg acgaaggatc aggagggcgg tccggttaaa gagaaggccg
    15301 tcatgaagcg cgagcactat ctggaagcga tggagaaggc cgaaaagttg attgaggatg
    15361 tcgagatgtg gctgggggat aagcccgaca ccaccaaagt gacggatgag gcctttgaca
    15421 aggaagagat gccggaaggt gtcgcgctga cacccctgca gaccgcaatg gaagacatca
    15481 tcggcgatct gctggagatc gatgagaaga tggacaggga ggccgacgac ggtgccatca
    15541 accaggcgtt gccggacatg gagatgggcg ctctgatcac cgagggcgac acgaccacct
    15601 tctccgcaaa gggtaaatcg gggaacacga cgccggatca caaagaacag gacgggcggt
    15661 cgaatgtcgg gcggcagggg atgtcgagcg gtgaaagcgc cgcgggttcg gggacaatct
    15721 ccgaggggga cgacaacatc gaggcgcgcc ggacgcagga tccgacccag tcgggacagg
    15781 tcgtggcgga tggcgaggcg caaaccaagg ccacgggcgg cggtaagctg ggttccggaa
    15841 aaggcgacga ctggggcgac agcggcggta ccgaacgcat ggacgcctcc gaagccggct
    15901 cgtgggcgca gttcatggag cagatggcgc gcaaggccga ccagtcgtat gcgcaggctt
    15961 ccctgaaagg gttgcgcacc gattcgctga aaacggccgc gcaccatatc cggcaggcct
    16021 cggacgcggt ttccaagggg gttccgattg gacaggttgc cgaactgcgg cgcaaggcga
    16081 tcggcgcttt gaagaaagcc aagacggaac tcggcgaggg cagcgcgact acgctggacg
    16141 gcggcgtggc cgtctcgacc ctgacggatg tgatcgaagc ggaacaggat ctggcccccg
    16201 ccaagtaccg ggagctgaac gccgattatt acaagaagct caacaaggaa ctgtaacgtg
    16261 tggtcccgcg tgtgcgggct cagacaggga tgcgtatgaa aacgatgatg cggaacgcga
    16321 tacggggtct cctcccggcg gtcttggcag ccgggttgtg ctgccgggcc gcggaacccg
    16381 gcgagtgggc ggtccgggac gcgacgatcc gctttgtagt ggggctgact cgggcgccca
    16441 gccaccccag cgccggtttt ttcgtgaaac tccctgacgg cgggagtctg ccgggcccct
    16501 ttcccgaaac gctcgcgttt gacgaggcgg ggaacccgct gacaagcgcg gtgttgtggc
    16561 acgctaaaga gatcggctgc gccgtggtct tcgcggcacc gaaagagggg aagaccgcgg
    16621 ttctgtatgt ccggggcgcg aaaaaactcg caacgtggac tccggcaagc gggttgaccc
    16681 ccagtgccat ccgctgtgag gtcaacggaa ccagcgccaa agccgcggcg cagaaactaa
    16741 gccggttggg ttcggtggat gccaagaccc attttcacaa tcaggcttgg tcagcgggaa
    16801 cctggcaggg cgaatcggtc ccgatggcgg tctgcgagtg gaagcccggc gggaacgcca
    16861 tctatctgct ggcccacatt gaggtcacgg cccccggtcc cacatgggtc gcgccgatga
    16921 tccgctccgg cgcgatggag atcgtgattg acggcaaacc ggttccgctc tccaagaaaa
    16981 acgagaagca gggcggtttg gggggcgagg ttaacctgac ggccggtctg catcgggtgg
    17041 agcttttcgg attcagcccc agagggcagg ccgtcggtcc catgatgttc acctggcgca
    17101 cgtcgaagac gaccatggag gagttgggcg ggcctcgtgc cagcgatttg cggtatcccg
    17161 gcacgtcgat gtatgaaacc gccaaaatca aagacgagca tgtggtcaaa accggcgact
    17221 gcaccctgtt cgagatccag tcgcagacgg ggttgaccgc cgccttcgag gccgtgcccg
    17281 actgtctgtt cgcgcttgag ggggaggagc cgcgggtcgc ctatacgttg aaagcctgga
    17341 caaaagggaa tccgaaagag acccgctaca gttggagttt cgaggaggcg ccgaacgcgc
    17401 tggccgacgg gccgacgacg cactggctgt ttaaggggtc ccgccacagc cggatcacac
    17461 tgacggccga ggcggcgggt cggcgcgcga cctgcaccac aaccctgttc gcctacacgt
    17521 tcgacagcag cagtctggat gatgaggaga cacggcacgc attcaagcgg gcgtgttaca
    17581 gcatgctgaa ggcgtatccc gaaaaggctg atccgactgc cgcgtgggac gacgccatgc
    17641 tgaacaacct cttccgcgtg ctggatctct ccggtgccaa tctgttggtc gaaaccatcg
    17701 tgacgcaacg gtggcccttc ttcaagaaac ggctgacacc cgaacggcag gggttgctgg
    17761 aggatatctt cctcttttcc ttagggccgc gtaagccgca ggaggccatc cggtgggcat
    17821 tgaaactgag ttctgaagag ttttccagtg gtcgtgccgc tgtattgaag ttgaaggccg
    17881 cagaggtgat gatgttttac ctcaacgatc tggcgggcgc ccggaaactc atcaccccgc
    17941 ttcttcgtga cagcggcgag gcaggggagt gggccaagat ccggatgggc gatctggaat
    18001 tcctcgaacg caacatcaat gccgcgacgc aacgctatgg cgacgtgcag agtcgtgcca
    18061 aggccacggc cgcctcgctc acgccgaagc ggctttcgga cctctcggcc acgggtgcgg
    18121 cgcgcgcgcg gcggtctggg ggtaagggcg gggagtccgg gcccgcgccg atggcgcagc
    18181 ccgccgatct gtcagcgtgg aagctctccg cgatccgcga cgccgcagcc tctgagcagg
    18241 tcaatattct gatcgaacag ggcttttatc tggaggcgtt ccaggcgttg caggcgtggg
    18301 agcgaatctt ccccctctcc aaactgacgg gagactatct gctctgcgag gcgaagctct
    18361 accgggcgct gaaggattcc aagcgggcgc gtgtcatctt gagcgcctat tgcgatcagg
    18421 tggacgcttc aaattttctg gtggcggcca tggagttgaa caagcagtgc atgatggatt
    18481 tgaacgagcc tgccgaggtg atcgaaaaat acacggagga gattcgcaga cggactttcg
    18541 cgggaggcga tcaatgagcc gtcgcgcgtg gccgttggaa gcggacagaa gggagaagcg
    18601 gagatgaacg gggtgtttgt acaatgcgtg tggggggtgc tgctggcgtg cgcgctttcg
    18661 gtaaccggcg ccgaagagcg ttcccggctt tatacggcca cggacccgtc ggccacgggt
    18721 ggactgaaag ggcggatcgt cagaccctcc cagccgatcc ttcaggtgtt ggccatgccg
    18781 ccggatgaac cgcgtttcgt ttatcagggc accgtgacgg gcggggaccg gcaggagttt
    18841 ctgtttcaga atcttccgat ggcgaggtac aaccttttcg tcatttatga aaatgagttt
    18901 ttcgagggtc ttgagttgtc gtctgaaccc gacaccctga cgcccaagga ccgcgagagc
    18961 atcacccgga tcgtgaacgc ctcggatccg tttttcaaca ggaaggtcat ccaccggatg
    19021 gccggaacca cggggcgggg caatttcgcc cgctgcgtgg tgacccagta ccgggacggt
    19081 ccggggatgg tcaagggtga tgacacagcg ttggaggctg gcaagggacg ccggacattc
    19141 aagctgatct ggctgaagga tgtgggcgtg ggctggcagg tggtgcaaaa gcgggatttg
    19201 tatcccgtga cggtggaact gaagctgctc acaccggccc atcattattc gaaacaactc
    19261 tcccgcatcc gggtgacgaa ccgcatccgg gatttaggcg agctggatct caggaaagaa
    19321 aaataatccg cctgtcacgg gcaaagaaag gaacgtttta tggcgagtct attggtgaca
    19381 aaagggcagg ggcagggtca ggagatcgtg ctgaccgggg ccgaaatcat gatcggacgc
    19441 gaggaggact gcgacatcca gcttttggat gattcgaaaa tctcgcgtca tcatgccaaa
    19501 atcacgaagg agaacggggt ctgggtggtg gccgatctgg acagcgccaa cggcacgttc
    19561 gtgaacggcg aggcggtcgt gagcgcgccg ttgaacgaaa gcgataccct tacgatcggt
    19621 gattcggcct ttctgttccg ggcggacgct gccgcggctg cttccgaacc tgcgggtgag
    19681 gacagcgggg cgaaatccct gaccgacgcc gaggatctga aacgggtcga gcggatgcgc
    19741 gaatccatgg acgccattcg ccgcgaggtg ggcaaggtca tcatcgggca ggaggatgtg
    19801 ctcaaccaga ttctgatgtg catcatcgcg ggcgggcacg ctctgctgat cggtctgccg
    19861 gggctcgcca agaccctgac cgtcaacacc ctcgccaaag tgctggacat gaagtttaag
    19921 cgcatccagt tcacgccgga cctgatgccg tccgatatca cgggcaccga ggtgctggag
    19981 acggacccgc agactcagca gaagcagttc cgtttcatgc aggggccgat tttctgcaac
    20041 atcctgctgg cggacgaaat caaccggacg ccgccgaaga cgcaggcggc actgctggag
    20101 gccatgcagg agaagcaggt cagcgtgggg aaagagatct ataagctcga taagcctttc
    20161 tttgtgctgg cgacgcagaa cccgatcgaa caggagggga cctatccgtt gcccgaggcc
    20221 cagcttgacc gcttcatgtt caacatcttc gtggattacc cgacgtacga agaggaggtc
    20281 acggtgatcg accggaccac gatggccgtg gggtcagagc cgaacgtcgt gctggacaaa
    20341 caggccgtga tccacttgca ggaagtggtg cggaaaattc cggtttcgca gcatgtgatc
    20401 cgttatgcgg tcagtctggt gcgggctacc cgtcccggtg acgagcaggc cccggagtgc
    20461 acgaaaaagt acgtgtccac gggtgccggt ccgcgtgccg gtcagtttct gatcctggcg
    20521 gcaaaggcgc gcgctgtgtt ggaggggcgg atccatgtga gttgcaacga tgtccgcggt
    20581 gccgccatgc cggtgctccg ccatcgtatt ctgaccaact tttccgccga ctccgagggc
    20641 atcaactccg tcgagattgt caagcggctg cttgaggagg tcaaagaacc cggtgcggaa
    20701 acataccaaa agtaggtgtc cgcaaacgcg cgcggccacc ggtcaggttt tcgagacggc
    20761 gcgtcttccg attcgggtgg gccggctgtt gcgaatgtgg gcctgcgtac tggccgggtt
    20821 tttgcctgcg gttgctgcgg aaaagcggtc gcccaacgat gcggcggccc caatccgcgg
    20881 ggttgacgcg ctgccacggg aacaacgccg cgccttcttg cgcggaacct ccgcagattg
    20941 gcggaacgac acggacgctg cccgcgctct cgcgccgccg ccgattcaga aggcgtatcc
    21001 cgcggacgcc aagcgggtgg atctggtggc gccggagcgt ttcacggtgg gagatgtgag
    21061 cgcgcgcgag gtgatcggta accgccggag tcgacgtgag ttctccgcag cgtcgttttc
    21121 gctcgaagaa ctcagctatc tgctgtgggc cacgcagggg atcgcccgga tcgatcggga
    21181 cggggcggga caggttacgg cccactatcg gactgtccct tccggagggg cgcggcaccc
    21241 gtttgagagt tacgtggcgg tctcgcgcgt tacgggcttg gcgcccggat tgtaccgtta
    21301 tctgcccgtg gagcatcagt tgctgctcct tcgcgaagac gcggacatcc atgaccgggt
    21361 gatggcggcg tgttacgggc agacctttat cggtcaggcc gctgtgacct tcatctggag
    21421 cgcggtcccg taccggaccg aatggaaata cggcgtcatc gcgcaaaaac tcattgcgat
    21481 cgaggccggt catgtctgcc agaacctcta tctggccgta gagtccctgc gggctggggt
    21541 ttgttcctcc atggggtaca gtcaggcccg gttggatgcg ttgctcggac tggacggcac
    21601 ggaggagttt gtcgtgtatc tggcatccgc aggaaaatgc ggggaggcac cggattcggg
    21661 cggaaccccg gaaaaatgag aggcaacgga gagtgcggtg aaaccctctg cgaacagttt
    21721 tgagccgttc ccgttccaat cggtagaggc gttgcgcgcc agtgcccgcc ggttaaaact
    21781 ggatctgccg ctgtccggcg aaaccgcagt gctggcagaa ccgctccggt tcggcggacg
    21841 catagcgccc aaccggtttt gtgtccagcc catggagggg tgcgatgccg agccggacgg
    21901 cgcgcctagc gtctggacac gccgccgtta ccgcagatgg gcggagggcg ggttcggggt
    21961 gatctggatg gaggctgcgg cggttgaggg ccgggcgcga tccaatcccc gtcaactgtg
    22021 gatcacgcgc cggaacgtca gggtttttgc cgagatggtc gcggaactcc ggcagtgtgc
    22081 ctgcacgtgc tggggccatg agatcgcgat cctcctgcag ctggcccatg cgggccggta
    22141 ttgccgcccc gagggcgtgc ccgcgcccgt gttcatcctc cctgatccgg acccggcggg
    22201 gatcccggag agtttggcag ggcaaacggg gctctccgac gaggagttgg atcggcttca
    22261 ggatgccacc gtgactgcgg gtctgcttgc gatggaggcc ggtttcgacg gtgtcgacct
    22321 caaagcctgt cacggcgatc tgcatgcgga attgctgggt gccctccgga ggccgggccg
    22381 ttacggcgga tcgctggaga accgcagccg ttttctgcgg gaggttgccg tcagactgcg
    22441 ttccgcgcgg cccgaaatga tcgtggcttg ccgactttcg cctgcaaagg agggtgggga
    22501 agaacgggaa cggattcggc tgctggccac cgccggtgtg gggcttttca atatcgcgga
    22561 ctctggatgg acggctggcg accggaaaaa gcgggtgaat cctctgcggc ggttcatgcg
    22621 cctggcggaa cgcacgcagg ccattcagca gatggtgccg gagattcccg tggtggccga
    22681 agggttcagc cggttccgcc gattcctgcc tcaggttgcg gcaggggtat tgcggcgcgg
    22741 agggggctcg ctgatcggga tcggacgcgc cgcgttggcg gatcccggtt tggcggggac
    22801 ggtgctccgg aacggcgaac cggatttaga ggcatgctgt atcgattgcg gcgcctgcgt
    22861 ccagctggtc cgggacgggg ggcggtcggg gtgtgtggtc acggaccgcg ccgtctacgg
    22921 cgaagagtat gcccgctgcc gcgcgatctc gctggaacgt ctgcgtgaag aggcgcggcg
    22981 ttgccggggg tgcgaacccg cgccttgccg tgcgggctgc ccgacccgca tcgatgtgcc
    23041 tgcgtttctg cgggcgtttg ccaagaggga agacgccgcc gcgtacgaga tcatccgccg
    23101 gagtaatgtt ttgcccggat tgtgtgccag cctctgcccg gtcggcacct tgtgcgaggg
    23161 ccgctgtgtg gccggaacgc tggacggcgt cccggtcccc attcacgcga tccagcacgc
    23221 tgtctaccgg tatgcccatg cgaacggcct gaccggtctg cgcctccctg aaaagccgag
    23281 cggccggaag gccgcagtca tcgggggcgg tcctgcgggc gtggcctgtg ccgtgacatt
    23341 gctggagcgg gggcatcagg tggtgctgtt cgaacgggcc tcccgcttgg gcgggacacc
    23401 tgagctggcg atccgcgaca gccggttcac cggtgcgcac gaagaagcgt gggcggtgtt
    23461 gatgcccgcc ctgcgcgcgg cgcgcctgac gatccggttt ggggtcgagt tcggtcgcga
    23521 ggtcacgccg gattccttga ggcggaaagc cgatgcggtt tttttagcgg cgggcgtatg
    23581 gggggagcgg tccgtgggga cggcggaagg ggttgtcgca gggctggcct tccttcgcca
    23641 aatcaaaacc cggacgcgtc aaaccgtccc cgaacgggtg atcctgctgg cgggagggga
    23701 cagcgccatg gattgcgcgc gggtggcgct ggaggcggga acgcgggaac tgctgatcgt
    23761 atatgacggt ccgctgtccg agatgcattg gcacatggct gatgcgtggt tcaggacggc
    23821 gggcgtgcat tttctgactt tgacccgtcc gctcggctat cgcaccggga aggacgggaa
    23881 ggtgacgggg ctgcggatcc ggaaaattct ggaatcggat tcagacggcg ggcctgtacc
    23941 ggaaacgctc cttgaagccg gactggtcat cgaggccatg ggtctgggac tggaggagtc
    24001 tctcgtgtcc gccctgcccg ggtgcgcgtt caatgcggag gggttggtca agaccgctga
    24061 aggcagcctg cgtgtcagca gcctccccgg gtttttcgcc gggggcgggg tgatcaatgg
    24121 cggcgcgtct gtggcgcagt gcgtcgcgga agggatgcgg gccggtatcg aaatggatgc
    24181 ggagttgcgg ggctgaggat ttctcggttc agtgcgtacg gttgggagcg cgtttagaaa
    24241 aaaagacaag cacgaaacgg aacgagaagt ccggggtgtt caggcacgct gaatcctgtt
    24301 cggtggcggt gaaggctgtg gtacggaacg gaggatatcg atgaacggaa gaaggatatg
    24361 ggcattgggg atggcaatgt ggttggtgcc gggcatctgg gcggaaggct agccgatgga
    24421 aaagggcgag gctcagatta tcaagtcgcg gatgatcccg tatcccaaac aggtgacgat
    24481 cacagccggt ccggtggtca aggtcgaccg tgagcttgcg gtgacggtca cactcaagga
    24541 cgaggactgc ggcctgaaaa aacgggtttc aaggctgttc aaatcctatt tcggcgtttc
    24601 tcctaaactg accttcaaga ccgatgcatc gctcgtgatc ccccaagacg gctatcgctt
    24661 caagatcgcc tcgggcagtg ggctggcgat cgaggcgacg ggtgagggcg gggtgttgaa
    24721 cgcgctcaaa accctgcggc aattggctga gccggagcgc ggtgtcgaga agatgcgctg
    24781 ctattttttg ccggagatgg cggtcgagga tgcggcgcag tggccgacga tgcagtactt
    24841 tcatgaaaaa ggattcccgg tgctcgcctg cccgtggaaa aacacggaag gcgtcagaag
    24901 ccaggcgcgt cttgtcaacg aaaaaggcat gacggggatg ttgtgtacaa cgtggaacta
    24961 catgcacagc ctggacatgt accgcatgct ctatgcgggc gcgcatgcga cgtggggata
    25021 tgacgggacg ggctggaatg cctcttccgc ctttaaccgg catgtgcgcc agatcggttg
    25081 ggacataccg gtcaaacgct accgcgacac gggcgtggcg cagtggcagg tgccgccgga
    25141 gaacgcgctg ctgcattgat ttctcagaca acggcgccaa gagaagaagg aacgatgaaa
    25201 acagaagaag tgaaagcgat ggcccgcgag tgcggtgcgg atctggtggg tattgcaccg
    25261 gtcggacggt ttgcggagga gccgccggaa cggaatcctg cggcgatttt tccggagtgc
    25321 cgctcggtga tcgtgctggg gcggcgcatt tgccgcggtt cgctgcgcgg cgtagaggag
    25381 gggaccaatt tcggcagtac ctaccagtgt ttcggttata cgtgggtgga agatcatttc
    25441 ctgtcgcaga cctcatatga cctgtcgctc cggatcgagg agcagggttt tgaggcggtg
    25501 ccgatcttcg cttacaacgg ggcggggatg cccaagggcg tgccggtcgc gccggaccgg
    25561 cccgcgccga acgtctacat cgatccgcgt gtggcggtcg tggctgccgg tgtgggagag
    25621 atcgggctcg gcgggtttct gctcacgccc gaattcggca cccgtcagcg tttcgctttc
    25681 attctgacgg atgccgaact ggagggggat gcggtgcggg cgaaaaccct gtgcggcgac
    25741 tgcggtgctt gtgcggaggc ctgtccattc ggcgcgatcc atctggacca gattcaaacg
    25801 atcacggtgc agggacagga gatgccggtg gcacgggtgg agagcggatt gtgcgcgacc
    25861 tgcccgaacg gcgcgtttct cgcggctggc cggggcaaag agccggaccg catcgcggcg
    25921 gcgtgcggcc gcgagtgtct ggtgcgtctg gagcagtccg gaaaactcgg caaccgtttc
    25981 gagcagccgt tccgcaaacg cgagccgtgg cagttggacg cgctcaaacg gccgctgacg
    26041 cgtgacgccg caggtgtgtt gcgggcttcg gatgtcgggt gcggcgcggc gggcggcacg
    26101 ggtgtggtgg cggtgcatgc cacgggtcag atcttttcca aataggcggc atatgacact
    26161 gacatcacag cagatcaaag agttcgccgc ctcgctgggg cggccctgtc tggtgggggt
    26221 cgcggatatc ggacggttcc ggagcgcgcc gcggcagatg catccggcca acattttccc
    26281 ggagtgcaag tcggtgattt ccgtggtggc gccttttccc cgcgggaccc tgcgcggcat
    26341 tacggagggg acgtattggc cgaattacac ctactactcg tacaaccggt tgaacaatct
    26401 gttccggccg cggatcacgt atgcgctgtc ctgtttcatc gaggatcatg gatgggaggc
    26461 ggtgccgctc tatcccgccg tgccggaacg cggcgggatg cacaggccgg tgaagccggg
    26521 gcggcccgcg cgggagatca acctgaacgt gcggatcgcc gcagtggccg cggggctcgg
    26581 cgaaatcgga tggtccaagg tgttcctcac ccgcaagtgg gggccgcgcg tacggatcgg
    26641 aacgatcctc acggatgcgg aactggagcc cgatccgctg attaaaccgg gcacgctctg
    26701 caaccgctgc atgaaatgcg tccggacctg ccccggacgc gcgatcccct cacccgggga
    26761 gaggccgccg gtacgggtgg agatcggcga tcaggtttac gagtggggcg atgtcagcat
    26821 ggggcgttgc acgttgacgc atcacggtct gaaccggcag gtgtcgccgt ttctggtcca
    26881 ggattttccg acagtggatt tcaaggttcc ggagagtcgt gtctcggagg aactggccta
    26941 ccgcatggcc tatactctgg ccaccggaac ctgggtggcg tcggaggagt tcccgcacgg
    27001 caacatcgtg gactattacc atcagattct cgggcacgtc gggtatttcg cgatctgcgg
    27061 tgcgcaggga tgcgtccgcg gctgcatgga cagtctggag aagcgccacg cgatcgaaca
    27121 gtccgctttc aagaccgcgg tttcgccgcg cgggcagtgg acactgcggc agccggagga
    27181 tgaggagtct gcgggcattg ccgagggaac gtgcgcggag ttgtaccggc agccggattt
    27241 gcaggcgggg cgctggtcgt gaggtgcggc tgcggagcgg cacgagaaga aagtgacacg
    27301 catacggaaa gggggaagga atgaatcgat ggtgcgtagg gggcatgctg ctggtttgct
    27361 gggtcgcggg atgggtgcag gcggaaccgt tggagcaggg cgaggcccgg ttcctgaagg
    27421 cccggctggt tcccgcgccg cagtcggtca cgctcacgga cggaccgcgc gtgctgttga
    27481 acggagcgtt gcgggtgtcg atcgcctgcg tgacgaacgg cgaagccgcc gtgacggaaa
    27541 cgcgtcgggt tttcaaagag tggttcgggc acacgcccaa gatcggcttg cgtcatgcgg
    27601 atgcggacgg gttgccgcag aagccggacg cctaccggat cgaggcctcc ggccgaacgt
    27661 tgttgatcga ggccgcggat tctggcgggg tccgcaatgc gttgcggacc ttacggcagt
    27721 tggccgaacc attgcgggga acgcgtgaag tggcggagta tgccattcct gcgacgcgga
    27781 tcgaagacgc gccggtcatg gccttccgcg gattccacct ctgctggttc ccggagaaca
    27841 cgccggtgca gatggagcgt ttcatccggc tggcagccta ctacaagatg aactatgtgg
    27901 ttatcgagcc gtgggggatg ttcgtttcgg gtcgtaaacc gggcttggcc tggccggagg
    27961 gaacgatgac ccgcaaggcg ctccggcact tggtgaaagt cgcggactcg ctgggggtga
    28021 ccctgattcc gcagttcaat gcgttgggcc atgcttcggg ggcgcgcatg agctgccaca
    28081 aacatgcggt tctggatctg aacccggagt tgcagccgct cttcgagcca ctggggtgga
    28141 gctggtgcct cagtaatccc gaaaccattc gggtgattga tgaatgtgtc acggatctgc
    28201 atgaggcctt cggacggcct ccatttttcc atgcgggatg cgatgaggcc gatgacatgg
    28261 gcacctgccc ggcttgccgt cggacggact atcccgcacg ggttcgggac catctgacgc
    28321 atctgcgcga cctgctggcc gcgcgcggat gccggatgat gatctggcac gacatgctgc
    28381 tcaggtcggg cgatccgcgt tggaaagggt tttacgcgaa cgggaaggaa ggggccgaac
    28441 gcctgttgga ggggttgccg cgcgacatca ttatttgcga ttggtactac ggtgcgccca
    28501 acaaagaggg gacgtggccc acgctgcggt atttcaagga aaagggattc ggtgtgctcg
    28561 cctgtccctg ggagaatgcc gagggatacc gcgggctcgg caaggctgtg gtcagcgaag
    28621 ggctcgaagg catgctctcc acgacctggc accaccttta cggccgggac atgatgggaa
    28681 tctatttccg tgcggcgcat gcgatgtggg gtacgcgacc caacggcggt cacgagtatt
    28741 tggtgttcgc gcaccatctg aggcagatcg ggtgggatgt gccggtgcgg acttacgagg
    28801 acacgggcac ctatcacacg caactgccgg aaaagaccgc ttcgccgcat taatcctaaa
    28861 aagggcagtt gaacgggtgg aattctagca aagccccgta aacattgggt tttgcggtgc
    28921 gcccgacgca ccgtattgag atgcgggcgg agcccgcaat ctcaaaggct gtgagactgt
    28981 g
//
LOCUS       DAOSLE010000017        28662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_1837_length_28662_cov_7.956409, whole genome shotgun sequence.
ACCESSION   DAOSLE010000017 DAOSLE010000000
VERSION     DAOSLE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 28662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28662
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1837_length_28662_cov_7.956409"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2091
                     /locus_tag="PK576_02475"
     CDS             127..2091
                     /locus_tag="PK576_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOE60486.1"
                     /translation="MGSMGTTESRKSNQSILSILSILFLCAFPAYAEVTLKVTPSRQN
                     IYLGESFNLTIEVGGADRGLEAPDLSGLPPSDVQFLGQHSNSRSSISIINGRMTREVY
                     EGRVFAYQIKPHAEGAFRAGPVRIKAGDKIITNPGPLVQVKGIEAQESVLVSLRASST
                     SVLVEEPLTLTLAVAVTELPEPYAAENEPLHPNRLPHLSAEFLELRQNTAGLKGPDLN
                     QILNGLIDQSGQQPAFAINEYTTQGMGSGFGRLFDPDPFRPRPIRFRLPHTRETINGR
                     KYRVYTLTLNYTPTQEGEFTFGPVLFKGEVIVDVSADRKAITRDIYTIGPAVTVRVVP
                     PPDEGRPETFIGAVGRSMQVSAALDATVCKVGDPLTLTLEISGAISVSNLRTPILNLQ
                     PELTRDFRIYDENVKADTLPNGKRFKYRVRPTREGTLEFPPIRLTYYDTEAREYRTLT
                     TAPIPLQARPTTQIATPDPDRTDVGLTGTLESHTRPLPAGITLDTQGTRPDPLLPPGR
                     VILLLMTAGPALCLLAFSASPLAACAAAVRTRRRHAGALRRALAALGRTHTPDRAART
                     VRRYLTDRLDVAGGALTPDEIADLLQQRGVPVNSATACRDLLARLDEAIYRPDAPIPD
                     TTLHRDLIALLPEIDAALAETCRVPTKDEL"
     gene            2091..2810
                     /locus_tag="PK576_02480"
     CDS             2091..2810
                     /locus_tag="PK576_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60487.1"
                     /translation="MSAFFNIILARADAVSGMRRRALVTISCSLFACHGVFALPGDDT
                     ARVFIWEQANAQIAVATTPEAYQKAAETYNRLVADNVCNGPLFLNLGNALVMAGDGIN
                     AAAAFSRAERHLGTTPETRQGLAAAFALQSGRASNDLPWDRIAFVWHYAFPCRIRAYA
                     ALCGWCLLWFGVLCLIRGRHRDRHTFLRSLAETCTLTGGLLATVFAASVLITLAQERH
                     DATTWGARVFLASTPETEGSP"
     gene            2807..4141
                     /locus_tag="PK576_02485"
     CDS             2807..4141
                     /locus_tag="PK576_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOE60488.1"
                     /translation="MTAPCTGRRIRHMKTSLRTLAATVVLLFGTGLPLHAGELVSGRL
                     YTVPETIYVNQAFEIYFELEVTFGSEVEDLRIRDFPNQPDLLTVGRLVSSSRNRTSRD
                     GKTLDTLQYKATARCHKPFHQTFTPLVQCMLVERRNTGFFSHWQSFPKQMRLDPFTLA
                     VRDLPDQGRPEAFSGAIGTFRLTGRLSQTEAHPGDIITLTLNLKGKGWLGEQTVMPAP
                     AVGPMFKRYPVKELLREDAHLRTEQIFIPQTTNATEIAAVRFHYFNPATGQYEASVAG
                     PFRLTLRDAPAEPVAQTIRMIDTVRPTTASEPPQTISIRQVNRSVRHALPLLIGGAGA
                     LAAFFIMFLLGAGRRRIAIPLALLALGVSLGAAYRIHIRADAATRNLTEQVDVRLAPA
                     RAAFQLFSLPAGTEVIPLETSASWVRIDADGRRGWIRAEALGDPTTVPKGAP"
     gene            4138..4869
                     /locus_tag="PK576_02490"
     CDS             4138..4869
                     /locus_tag="PK576_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOE60489.1"
                     /translation="MKERLDTLMVRAGLAESKERAQRLILAGEVFAAGQLATKAGHKY
                     PVDIELTVKAQPRFVSRGGEKLEGAFCAFPEFNVTGRVCLDVGASTGGFTDCLLQHGA
                     ARVIALDVGKGQLHWSLRQDPRVQVMESFNARYLRLSDLPEQPQIGVTDVSFISLRHI
                     LPPMSDVLPSGGEIISLIKPQFEAGRENVPGGVVRDPAVREAVVESIRAFGTELLHLN
                     WLGCVASPLRGPEGNVEYLAWWRKP"
     gene            complement(5000..6667)
                     /locus_tag="PK576_02495"
     CDS             complement(5000..6667)
                     /locus_tag="PK576_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOE60490.1"
                     /translation="MRLTRLMMIRPYRNAKRMRQIFVVFARCGWACLKAKIQARRSVR
                     AGGAPADLNARIGENLRKALESLGPTFVKLGQILSTRRDILPDGIALELARLQDNVAA
                     IPAQDAICVVETEYGCPLRDVFSEFSTVPIGAASLSQVHEARLASTGESVVVKIQRPG
                     IQTIVATDIEILTRFLPLLERYVEEFRRLNLRSMVNEFHKTILMEMDFHREMHNGLKF
                     KTIFAGDPTVRIPVMYKDLCTDKLLVLEKLDGVKIDQLDELVRQGVDRDTLSKHLVSL
                     MAKQIFRHGLFNADVHAGNIIVLPDHVLGLFDFGMVRTIDPETRQLMTDMFVAVMTKD
                     AVRLSKLLAAYAEPGSQVDVKQLARELHELIVFYYDLPMEDLRLDVLLETGINLLATH
                     KLIIPQQLIMLCRTTVMTESIVLMLSPRINFVKALAPYVREAIRSRVTFSNLSGGISQ
                     FAMDSADTIKALPTAIGTIMTNLSEGKQVVAYRHEGLEKHVRQVSRMTAQLALSIMGG
                     ALFLGSALILALQPDGGGTLRSTLGTAGLTVAVLLFVGVSLSMLRKK"
     gene            complement(6791..7108)
                     /locus_tag="PK576_02500"
     CDS             complement(6791..7108)
                     /locus_tag="PK576_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60491.1"
                     /translation="MKKEGVCAVEIVLGLAVTGQKKLVAAVESLVKKGELSKQQSQAF
                     LREVQSEGAACKKKVGDQFRDSAKKVLGELGLVTADDLAKIHKEISGLKAAMKAPRAA
                     TKA"
     gene            8204..8277
                     /locus_tag="PK576_02505"
     tRNA            8204..8277
                     /locus_tag="PK576_02505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8236..8238,aa:Gln,seq:ttg)
     gene            8292..9236
                     /locus_tag="PK576_02510"
     CDS             8292..9236
                     /locus_tag="PK576_02510"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOE60492.1"
                     /translation="MIVFSGTANRPLAEKIMTYLEGRLGDIHIRRFPDGETHVKIVDN
                     VRGMDVFIVQPICGQPNEMLMELLIMIDAARRGSAARITAVLPYYGYARQDRKDQPRV
                     PITAKLVANLLVAAGANRVLTMDLHAQQIQGFFDIPVDHLHAAPVMVKYLREIELKKP
                     LVVSPDTGGAKAAYAYSQMLGTGLAIVAKQRLGDAEVDAFSVVGDVKGCDVIMVDDMT
                     TTCGTLCAAAKILKEQGAASIRAAVTHMPLTDKGVQRLQADTGLTELVVTDTVPLREG
                     LDSAAIPVKLTVLSVADLLGEAIRRIHNDQSVSSLFRY"
     gene            9267..9947
                     /locus_tag="PK576_02515"
     CDS             9267..9947
                     /locus_tag="PK576_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOE60493.1"
                     /translation="MANEKIRMKAELRTEQGSGAAGRLRRAGSVPGAVNRIEGGTTLV
                     KIDAHAFKTMLRHHGREQMLVTLEMDDKEVSALMREVQYDAITGYPIHVDFGEVSLTE
                     KIRVALPLQLTGEPEGVKLGGGVLQQMLRTIDVECLPTDLVEHFEVDVSGIKLGQSLF
                     VRDLKLGDAYTLFTSRDGVVATVAAPEEEEEATAATPEAAAAATPEVITKGKKEEDGE
                     ASAPAAKK"
     gene            9972..10565
                     /gene="pth"
                     /locus_tag="PK576_02520"
     CDS             9972..10565
                     /gene="pth"
                     /locus_tag="PK576_02520"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE60494.1"
                     /translation="MIAGLGNPGEMYANTPHNVGFDVVDLLAERLGCGWKRSARFHAF
                     VSRTRCAEEPILLVKPQTFMNLSGTSVVPLLRYYGGEPDDLIVVLDDADLPLGMLRIR
                     AAGGTGGHRGLTSVIEALGSDAFTRVRLGVGREGRGSLADHVLGKFDEARQEKARAVV
                     EAAADAVQCLTEKGLNEAMNRYNGWRADAPLPPDDRK"
     gene            10578..11048
                     /gene="rpsF"
                     /locus_tag="PK576_02525"
     CDS             10578..11048
                     /gene="rpsF"
                     /locus_tag="PK576_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOE60495.1"
                     /translation="MMKKYEGLYIFAGSAKDDVLDKQVEKVLGEITRLSGNVISTDVL
                     GKRTFARPMNKRENGVYVKIRFELDPQHVTDLKNRYRLLEDLFRVQILVVDERRESVL
                     AQQEEAHKARAAARAAAAEAEAEAAAEAEAAAEAAAEAAEEEEEEVESQDERDA"
     gene            11080..11541
                     /gene="ssb"
                     /locus_tag="PK576_02530"
     CDS             11080..11541
                     /gene="ssb"
                     /locus_tag="PK576_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE60496.1"
                     /translation="MASFNKVILMGNLTRDPELRQTPTGAKVAELRLAVSETWRDKSG
                     QSKEVTCFVDVVVWEKLAELCHRYLSKGRPVLVEGRLQLDEWKNQQGETRSKLRVRAD
                     TVKFVGPPPARSGETPTAAQPAAPDASVPVADRTDYNVPDDLTEDDEHIPF"
     gene            11647..11832
                     /gene="rpsR"
                     /locus_tag="PK576_02535"
                     /pseudo
     CDS             11647..11832
                     /gene="rpsR"
                     /locus_tag="PK576_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490626.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            11854..12306
                     /gene="rplI"
                     /locus_tag="PK576_02540"
     CDS             11854..12306
                     /gene="rplI"
                     /locus_tag="PK576_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE60497.1"
                     /translation="MSTELLLLSDIPDLGQAGDVVKVAPGYARNYLLPKDLAAPVSPQ
                     ALRRLEKLRKEREEQTRLRLAEANAKAAKLTNASVTLRAKTVDGTRLYGSISAADIAA
                     AIETENKVTVDKSQIELPEPIKETGTFDISIILHDDVKPVIKVWVVED"
     gene            12449..13852
                     /gene="dnaB"
                     /locus_tag="PK576_02545"
     CDS             12449..13852
                     /gene="dnaB"
                     /locus_tag="PK576_02545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE60498.1"
                     /translation="MEAERGVLGSILLDAATDNRVLDLCIESGITADSFFSLPNRLLF
                     ETLSEMARASQIIDPLTLTERLRALNRLEEIGGAVAIQTLIDETPTAAHAEYYINILR
                     QKHLLRAVITCARDAERRCFDEAISADLILSEVEQKFLSIADRRDGAGLTWPQAITNT
                     MGHIDRLFSLGPGAVAGLPTGLANLDRKLKGLRNGEMIVLAARPSMGKTSLAMNIAER
                     VALGKDMNGRAMKGDHNRPHPVAVFSLEMSTESLAMRMLCGLAEVPSFKIDQGLVNPK
                     KINRQLTDAASKLAKAPIYVDDTGGLDVMDMRARARRMKKRYKVELIVIDYLQLCNCR
                     EFARQGRQIETSQISGQIKAMAKELNLPVIVLSQLSRAPEQRGDKTNKPKLSDLRDSG
                     AIEQDADVVLLLRRPCKASGDPEFDDKLLAIVDVAKHRNGPTGEVRLNFEDTLTRFSD
                     RTDQITEDDRFEPDPDS"
     gene            complement(13950..15332)
                     /locus_tag="PK576_02550"
     CDS             complement(13950..15332)
                     /locus_tag="PK576_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOE60499.1"
                     /translation="MGGNGVQVRFRAGDIPRAEAARALALDLNRAGYETFFAGGCVRD
                     ALLGRPVQDIDLATAATPDQVEALFPGETVAVGKAFGVVVARRNGFSFDVATFRTDGP
                     YRDGRHPEQIAFTNAEGDALRRDFTVNGLFCDPATGDVTDYVGGLTDLQAQVIRAIGD
                     PAARFREDRLRMLRAVRFAAVLEFTVEPRTREALEVWAPRITEVSPERVAHELNRILC
                     EAPLPSTALNLMRETGLLSPILPEVARLYGTAQPPRFHPEGDVWTHTCLMLDALPPPR
                     DLTLAWSVLLHDLGKPMTCREETDPATGAVRIRFPCHAPVGAAMAEELLTRLRQPSAR
                     IAEVRALVAGHMRGVESGKMRRATLRRFMGEPTFPRMLELMRLDIQHSNGDLTPWRQL
                     VEAYAAFVSEPVLPEPLVRGRDLIAWGVPSGPQVGKLLRELYDAQLEGQLATLEQARE
                     MVCRRRAAPR"
     gene            complement(15332..19177)
                     /locus_tag="PK576_02555"
     CDS             complement(15332..19177)
                     /locus_tag="PK576_02555"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOE60500.1"
                     /translation="MKKTGLSNQPPRYTAGQANAARTVRRLAGCLCLAGALSAAGLEV
                     WSLDDLQKVGSGIDGWEPDAVYTLMTNLDASATADWNGGAGFVPIGRDAEWGFTGVFN
                     GNGHRITGLTLNRPGESDVGLFGRIGTGGVVSNLCLTGITVAGLVDVGGVAGYLSEGL
                     IRECSVSGDVVGLETVGGVAGFVDLGGIERSSASGSVVADVGAGGLVGYLYEGGTIGE
                     SYAIARVSGTEDTGGLVGSGSGFNSVVSGAYWDTQASGQSSSWGGGTGKTSAEMRQAV
                     TFSGWDFSQVWGIVESVSYPYLRGLAVNGNEFVLTVASRGPGNVFVTPLLSSYTPGTN
                     VTVTAQPVAGVSEFVRWSGGVAQPTNPVTQVTMAGHVSLTAIFRSARGIGSVQELQRI
                     GRDPDYTLNGRYWLTGMLYAGETAGWPDGFGPIGSMTTPFTGSFDGRSNVIARLKVDR
                     PEEDYAGLFGVLGPGAVIRNLGLTYAEVCGNGGLGALVGYNAGGTVERCFAEAQVNGT
                     YNGIGGLIGENREGTVNRAYAVGSVAGQSNVGGLIGLHADGGSVTQTYAACVVTGTSS
                     GVGGLIGWGDGITAGSFWDTEVSGQSGSAGGVPQTTSAMRQAVTYSGWDFIGDWQIVE
                     GEGYPRLRGFARACTVTFDALGGTVEPATAVVDLGGVYGDLPVPERTGYVWEGWRSGP
                     GGAGTPVTEDTVMRTVTNHTVYAHWRADALIVVGGASDAVPIGFTNAVQVSTNHCGDG
                     GTSVCLGGKGIIADGQMAGIEWSVTGPGLLSFCWRTSSEADWDVLRFYEAGTGSIHQI
                     SGIMNNWEEMSILVEGGQETPHLFRWEYEKDPQGDYVGQDCGWVDALRWVPFHPLTVI
                     HGSGGGAYSNGTVVAIQADSPPAYHVFDRWTGDTNSVADVLAASTTVTMSGTGMVLTA
                     SYQATGYPLTVIHGSGSGVYPYGSQVVIGAEVPEGKQFDCWTGDVATVADRFAPTTTV
                     TTTDSALCVAATYRVPLTVVCGSGSGWYAEGSAVMVAADPAPLYQEFAGWNGDATSAL
                     ANATAPSTVLTMPSQAVTLIASYRDSVARLLGCYGPAFTCEGVVDGLTGDAMSGAPSG
                     TPAVKLGGPGVVPDNGFTAFELQVWGSGTVSFWWRVSSEPGADFLRFLVDGTPVMEIS
                     GTKSPWTAVTNTVSGTGTWHTLRWEYRKNGAEASSSDAGWVDDVIWTGDVPQPWLTPD
                     ILLFAVTNDAFCIDFLGERGIPYQVQTNALLRTDGWGGMSVAPVRDGETNGVHRFRAG
                     MAMPGLADTLFFRIRAGGE"
     gene            19326..20045
                     /locus_tag="PK576_02560"
     CDS             19326..20045
                     /locus_tag="PK576_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE60501.1"
                     /translation="MPHRTLSLQQAARHIRIPERELFHLVQRGEIPFLRHGDEVVFEH
                     RRLDDWAQRRILGLPTKSLTQHHRETTAERTPHSKDDALVARLFRPEWIDPALPSRTK
                     PAVVRDMVALAVSTGLLYDDAAFQREVEEREAACSTAIGGGAALLHARYHDPFWASDS
                     FIVLGKTQQNIFFGAQDGGETDLFFLICCTDDALHLHALARLCMLAHGSLLLSDLRAT
                     DRADEMHQILLRAESELLRTL"
     gene            complement(20052..22034)
                     /locus_tag="PK576_02565"
     CDS             complement(20052..22034)
                     /locus_tag="PK576_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE60502.1"
                     /translation="MTPYGFRPHPQSHDRHLLLACVAVLLSAVIHVVLMVYFSDWTIS
                     GISSLRERGREWFTGDRVPPMRVETMRADPMYIGRKKPGERDTPSRGPIEVSEQVEAL
                     SQSAAPVLTAPPPIPREALQPGVPALKEAASPQIDTTPWMPRQEIAQIYDRTVVDEVA
                     TLPRREIPMIERVPDAPDIVPSIDLVGRRFGNDPEPPKPFEAAEVFDTEISAGTFKVP
                     APEAPATPAPVSTAATESRFAMQPGEKAGKSPTTGSAADTGSATAGPDGASTSEQQKR
                     IERELAAVSQQIAATEVSEEERKAREAQAQIAALQELADYQPIDDLLAVGLETYRDPA
                     DPGRIYFRIGIEPRRDRTVPTLDKDIVWVQDVSASMSEERMVFCRRAMTAALETMNPG
                     DRFNVVAFRDTTEMCFPDWVPVTQAHLKKASTFVAAMRAFGQTDVFGSLRTLMKLPRD
                     PKRPMIAFIVTDGKPTFGMTESAKIIGEFSKLNQGVVSVNMYGTQSKANVYLLDMLTY
                     GNRGGSVVLTGNRWDIPDSMAGAYTGLRYPVMSDIQVTFDSVSQSEVYPKRTTPLYKD
                     RQIQLHGVCPDTTEELVFQIRGLAADKGYDSIFRLSLSRHAKLGTAAVRQRWAYQKLY
                     HLVSVYAAEPRQQVLAEMLKVNQQYGIPIPYESEMK"
     gene            complement(22031..24394)
                     /locus_tag="PK576_02570"
     CDS             complement(22031..24394)
                     /locus_tag="PK576_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE60503.1"
                     /translation="MRICRAQISAGMLLLAVCGVRAADPVEMRDRLQLADGLFRRSLF
                     DLAAREYVSLAETPQVPDRDNVLFRLAECYRRTGRAAEAAATYKQLSESFPDSPNVAR
                     ADLQRALLLLRAGGAELNVAAALFEKLTATGMPPEVRSAALYHLGETLEKLDKPAEAL
                     ARYEQLSREYAQTDYGMYAGLRIAWLLGKTGKPEDRRRAMGIYLDLAHKARDPKVAEE
                     ALFFAAQTALLDQRHEESANLFKTLKTKYPASPRIRESALSAGWANYYTGRYAEAFES
                     LDLIIGDAGHPAREEVLYLRANCLRQLERRAEAVKVYSQQLEAFPTGRLAGQAGYEKL
                     TTLYHDGAYEAVLKMTSENTRPVDLHADHVYWMNAEAALALQRYDAAVQYCRLLVDKC
                     PQSPLVKDALYRLGWLLQKQEAWESASSWYLQVVQRFPDDPLASRALYASGVCLSRLG
                     QSDAALRDWTALLTRYPECAEVAETLYQKAMEELRAKNVRAAAATLDERMRRFPNDAR
                     KAEVLFWRASAYRQVGEDAEAEKLFRACLAASPTKDFERESMLELGMLLQKTGRKPEA
                     ASFFQKLLDAPIAEKLGAERLAWLAEFQLEQKQPAAAIQAATRLVELKVDKGWTQTGW
                     TLLGRVHRAKGERDPALHAFTEALATGASTAYGAESALRLGELLSEAGRFDDAEKHLT
                     DAASRAATPDLLGLRAHAYAGLARNAERKGDSEAALRYHISVGILFDDPTLVPQSLHQ
                     AAVLLDKSGRAREAAALREELRTRYPASPLANPVPSTAAAGERKAGS"
     gene            complement(24391..24780)
                     /locus_tag="PK576_02575"
     CDS             complement(24391..24780)
                     /locus_tag="PK576_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE60504.1"
                     /translation="MTSMMDIIFLMLFFFITTSVFSQWEYEINITLPSAQSGKVPDRL
                     PGEIIINITRDGRVSVNQQDLTLDALRARLDRLARYFPGQPVVLRADKETRYEDLIKV
                     VDTCRKADIWNFSMATSEEKPAATETP"
     gene            complement(24832..25605)
                     /locus_tag="PK576_02580"
     CDS             complement(24832..25605)
                     /locus_tag="PK576_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE60505.1"
                     /translation="MVNRKTVTGVLAFTGSLACAWAQEAAESAAAVPALINGYGMSLK
                     QAWEYGGWVMWVLAAISVFALAMVFYFMTVLRSGAITPRELLIDLVARVRANDLTEVR
                     RLCDRHPCPLASVVLAVLDCLRNVPQCDVALLRSTAEAEGARQADAIQGQTEFLLDVA
                     TIAPLLGLLGTVLGMLTAFGSVASDIASAKPVVLAAGVSQAIVTTVFGLIVAIPCMVF
                     YAWFRRRASRQIANLEAASSEVLTALIGMGMKAPFTGQD"
     gene            complement(25661..27052)
                     /gene="argH"
                     /locus_tag="PK576_02585"
     CDS             complement(25661..27052)
                     /gene="argH"
                     /locus_tag="PK576_02585"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOE60506.1"
                     /translation="MKKETASQKTIVGKIHPDVLAFTVGKDPVLDLDLAVWDCLGTAA
                     HVTMLSRMRVRPAVLGTAERNRVIAALAEVIREIRAGEFRITQADQDVHLAVERRLTE
                     RLGELGRKIHTGRSRNDQVAVDIRLHVRDQLLGLTEEVAEVAGELIRFGCRHAKVPMV
                     GRTHMQPAMPSSVGLWATAYAETLLDDLLMVEAAYTYNDRCPLGSAAGYGVPLPVDRE
                     LTARLLGFSAPHHNVFHASNARGKNEAVLLAACGQLMLSLSRMAEDLILFSMPEFGYF
                     TLPPELCTGSSIMPQKNNPDVLELIRAKAAVVLGYGSAANMILKALPGGYNRDLQETK
                     ALYMEGLTVTRAALRIMRLMVGTLTVRPERLRAGFTPDVFATDRALELVAGGMPFRDA
                     YQHVRSHLETLQEADPDRALAAKTHLGATAGLDFGGYRARVRDVRRWVRVRRTRLRAI
                     FSALLGGAADALG"
     gene            complement(27067..28311)
                     /locus_tag="PK576_02590"
     CDS             complement(27067..28311)
                     /locus_tag="PK576_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60507.1"
                     /translation="MGHDSRIRRLATAAGWVLSMFAAVGGEPYGPRRTRLAAPAGAIT
                     SRDGSIVVRAPVAMGSYRAPVLVFVDRTRDELARATRLKLVTPSCPLEIVIGNRRDGD
                     TRVLGSRVREPGGGVFERIELPDPEAADLGRFRRAISLALLRAWMVEAGGREGVMRDV
                     PLWLVDGVVRHTGRETRQQDIDRTLLLWSRACLPPAAALFAMESVAASREPAVAAVLA
                     SWLLERRPDGLPFEKLLCAAAEGQEWRPDAVARILTGTEDPLAFDAALDLWFTALGRT
                     VVKPGLTSSGIVRRFRSRLLLYPAFYGTTWGYERPYLTFREAAMRSAEPEVQRRAAVQ
                     ARLIEMAAVGRDGMLLAVSEAYASFLQGVCSGEAQGELLRRLMEAEGMRRELELRTAR
                     GMVLQRADAGRATGAGPRRVRE"
     gene            28416..>28662
                     /locus_tag="PK576_02595"
     CDS             28416..>28662
                     /locus_tag="PK576_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HOE60508.1"
                     /translation="MLYFECGSKSGALDRTRLKQGLVEALDRLGPRQKVLVLPPDFTR
                     FHSQAGLLTQYVWEYYGDRLAAVMPALGTHSPMTEAQI"
BASE COUNT         5700 a         9684 c         8289 g         4989 t
ORIGIN      
        1 ctgcgtaagg cgctcgaacg cgaaaaagag cacgaagagg aaaagaaaga gcgcatgcgc
       61 aagatgccga tggtgccggg tgagagggac tggtaaacgc agatgttcgg gcgaaagaca
      121 aaagcgatgg ggtcgatggg aacgacggaa tcgagaaaat cgaatcagtc gattctttcg
      181 attctctcga tccttttcct ttgcgcattt cctgcctatg ccgaggtcac cctcaaggtc
      241 acacccagcc gccagaacat ctatctgggc gaaagtttca acctgactat cgaggtcggc
      301 ggcgcggacc gcggactgga agcgcccgat ctctcgggac ttcctccctc ggatgtccag
      361 tttctcggac agcacagcaa cagccgctcc agcatctcga tcatcaatgg ccgcatgacg
      421 cgcgaagtct atgagggccg cgtcttcgcc tatcagatca aaccccacgc ggaaggcgcg
      481 ttccgcgccg gtccggttcg gatcaaggcg ggggataaaa tcatcacaaa ccccggccct
      541 ctcgtgcagg tcaaaggcat cgaggcgcaa gagagcgtgc tggtttcgct ccgtgcctcc
      601 tccacctccg tactggtcga ggagccgctc accctcaccc tcgccgtggc tgtgaccgaa
      661 ctgcccgagc cgtatgccgc ggaaaacgaa ccgctacacc ccaaccggtt gccgcacctc
      721 tccgccgaat tccttgaact ccggcaaaac actgccgggc tcaaaggccc tgacctgaac
      781 cagattctga acggtctgat cgatcagagc ggacagcaac ccgccttcgc gatcaacgaa
      841 tacaccacac agggcatggg gagcggcttc ggccgtctct tcgaccccga ccccttccgt
      901 ccgcgcccga tccggttccg cctcccgcac acgcgcgaga cgatcaacgg ccggaaatac
      961 cgcgtctaca cgctcacgct caactacacc ccgacgcaag agggcgagtt caccttcgga
     1021 ccggtcctct tcaaaggtga ggtgattgtg gatgtcagcg ccgaccggaa ggcgatcacc
     1081 cgcgacatct acaccatcgg tccggcggtc accgtgcgtg tggtgccgcc gccggatgag
     1141 ggacgccccg aaacattcat cggggcggtc ggacgatcca tgcaggtctc cgcagccttg
     1201 gacgcgacag tctgtaaggt cggcgacccc ctcacgctca cgctggaaat ctccggcgcg
     1261 atcagcgttt ccaacctgcg cacaccgatc ctcaatctgc aaccggaact cacgcgcgat
     1321 ttccgtatct acgatgagaa cgtaaaagcc gataccctgc ccaacggcaa acgcttcaaa
     1381 taccgcgtgc gtccgacccg cgagggtacg ctggagtttc cgccgatccg cctgacctat
     1441 tatgacacgg aggcgcgcga ataccgtacc ctcaccaccg cgccgatccc cctgcaggcg
     1501 cgtcccacca cacaaatcgc cacaccggat cctgaccgga cggacgtcgg tctgaccggc
     1561 acgctggaga gtcacacacg gccgttgccc gccgggatca cacttgatac ccaaggcaca
     1621 cggcccgatc cgctgttacc acccggacgc gtcatcctgc tgttgatgac cgccggaccg
     1681 gctctctgtc tgctcgcctt ttccgcatcc ccgctcgccg cctgtgccgc agcggtccgt
     1741 acccgccgtc gtcacgcggg tgccctgcgc cgcgccctcg cggctttggg ccggacacat
     1801 acaccggatc gcgcggcgcg gaccgtgcgc cgatatctga ctgaccgtct ggacgtggca
     1861 ggcggcgccc tgacgcccga cgagatcgca gacctgctgc aacagcgcgg ggtgccggta
     1921 aactccgcca ccgcatgccg cgatttgttg gcgcggctgg acgaagcgat ttaccggcct
     1981 gacgccccga tcccggacac aaccctgcac cgggacctga tcgcattgtt acccgaaatc
     2041 gacgccgcac tcgcggagac gtgccgcgtc ccgaccaaag acgaactgtg atgagcgctt
     2101 ttttcaacat cattcttgcg cgcgcggatg ccgtgtccgg catgcgtcgc cgcgcattgg
     2161 tcacgatcag ttgttcgctc ttcgcatgtc acggcgtttt cgccctcccg ggagacgaca
     2221 cggcccgcgt tttcatctgg gaacaggcca acgcgcaaat cgccgttgcg acaacgccgg
     2281 aggcctatca aaaagccgct gaaacctaca accggctggt ggccgacaac gtgtgcaacg
     2341 ggccgctctt cctgaacctc ggcaacgctc tggtgatggc gggagacggc atcaacgccg
     2401 cagccgcctt cagccgcgcc gaacggcatc tcggcaccac ccctgaaacg cggcaggggc
     2461 tggccgccgc gttcgcgctt caaagcggcc gcgcctcgaa cgatctcccg tgggaccgga
     2521 tcgcgtttgt ctggcactat gcgttcccct gccgcatccg cgcatatgcc gcattgtgcg
     2581 gatggtgcct gctctggttc ggcgtgttgt gcctgattcg cgggcggcat cgcgaccggc
     2641 acacctttct gcgcagcctc gccgaaacct gcacgctgac cggcggactt ctcgccactg
     2701 tgtttgcggc ctcggtgctg atcaccctag cgcaggagcg tcatgatgcg accacctggg
     2761 gtgcgcgtgt ctttctggct tcaaccccgg aaacggaggg ctcgccatga ccgcaccttg
     2821 caccggacga cgcatccggc atatgaaaac ctccctccgc accctcgcgg caacggttgt
     2881 gcttctgttt ggcacgggtc ttccgctaca cgccggcgag ttggtctccg gccgcctgta
     2941 caccgttccc gaaacgattt acgtcaatca agcgttcgag atctattttg aattggaggt
     3001 cacgttcggc agcgaggtgg aagacctccg catccgtgac ttcccgaacc agcccgacct
     3061 cctcacggtc gggaggttgg tgagttcctc ccgtaaccgg acttcgcgcg acggcaagac
     3121 tctggacacc ctgcagtata aagccaccgc ccgctgtcac aaaccgttcc accaaacttt
     3181 cacgccgctg gtgcagtgca tgctggtcga acgccggaac accggcttct tctcgcattg
     3241 gcagtccttc cccaagcaga tgcggcttga ccccttcacc ctcgcggtgc gcgatctgcc
     3301 tgatcaggga cgaccggaag cctttagcgg cgcgatcggg acattccgtc tgaccgggcg
     3361 tctctcccaa accgaggccc atcccggcga catcatcacg ttgacgctga acctcaaagg
     3421 caaaggttgg ctcggcgaac agaccgttat gccggcaccg gccgttggtc cgatgttcaa
     3481 gcggtatccg gtcaaagaac tcctgcgcga ggatgcgcat ctccgtaccg agcagatttt
     3541 cattccgcaa accaccaacg ccaccgagat cgccgccgtg cgcttccatt atttcaaccc
     3601 cgcgaccgga cagtacgaag cgagcgttgc cgggccgttc cggctgacat tgcgcgacgc
     3661 acctgcggaa ccggtcgcgc agacgatccg gatgatcgac accgtacgcc cgacaaccgc
     3721 atcggaaccg ccgcagacca tttcgatccg tcaggtcaac cgttcggtgc ggcacgcatt
     3781 gccgttgctg atcggcggcg cgggtgcgct ggccgctttc ttcatcatgt tcctgctggg
     3841 tgccgggcgc aggcggatcg ctattccgct cgcgctgttg gcactgggag tgagtctggg
     3901 cgccgcatac cggatccaca tccgcgcaga cgccgccaca cgtaacctga ccgagcaggt
     3961 tgacgtgcgt ctcgccccgg cgcgagccgc cttccaactc ttctccctgc ctgcgggcac
     4021 ggaggtcatt ccgttggaaa cttcggcgtc gtgggtgcgc atcgatgcgg acggacggcg
     4081 cgggtggatc cgcgccgagg cgttgggcga tccgacaacc gttccgaaag gcgcgccatg
     4141 aaagaacggc tcgacaccct gatggtccgt gcgggactgg cggagagcaa agagcgggcg
     4201 caacgtctga ttctggcagg cgaggtgttt gccgccggcc agcttgccac caaggccgga
     4261 cacaagtatc cggttgatat cgagttaacc gtcaaagcgc agccgcgttt cgtcagccgg
     4321 ggtggcgaga agctcgaagg cgcgttttgc gcattccccg agttcaacgt caccggtcgg
     4381 gtctgcctcg acgtgggggc ctccaccggc gggttcaccg actgcctgtt gcagcacggc
     4441 gcggcgcgcg taatcgcgct ggatgtcggg aaaggacagt tgcactggtc cctgcgtcag
     4501 gatccgcggg tgcaggtcat ggaatccttc aacgcccggt atctgcgttt gagcgatctg
     4561 cccgagcaac cgcaaatcgg ggtgacggac gtctccttca tctccctgag acacatcctg
     4621 ccgccgatga gcgacgtcct gccttcgggc ggcgagatca tttcgctcat caaaccgcaa
     4681 ttcgaggcgg ggcgcgagaa cgttccgggc ggcgtggtac gagaccccgc agtacgggaa
     4741 gccgtggtgg aatccattcg agccttcggc acggagttgc tccacctgaa ctggctcggc
     4801 tgcgtcgcct cgcccctgcg cggtcccgaa ggtaacgtcg aatacctcgc ctggtggcgc
     4861 aagccgtagc ttgccgaagg cggtcacaga gtccgggcgc agcaggcacg ggtgcgtaaa
     4921 aagcgcaacg gccatgccga tcacgatccg cggaaagaaa ggtccggcgc ctcgctgacc
     4981 ggcacagccg cgccgggctt cacttcttcc gcaacatgga aagagacacc cccacaaaaa
     5041 ggaggacggc aaccgtcagt ccggccgttc ccaacgtgct tcgcagtgta cctccgccgt
     5101 ccggctgcaa ggcaaggatc aaggccgacc ccagaaaaag agcgccgccc ataatgctga
     5161 gcgccagttg cgccgtcatg cggcttacct gtcgaacatg tttttccaac ccctcatgtc
     5221 gataggcaac cacctgcttg ccctcgctca ggttcgtcat gatcgtgccg attgccgtgg
     5281 gcagggcttt gatcgtatcc gcgctgtcca tcgcgaattg actgatcccg ccgctcagat
     5341 tggaaaaggt aacacgggac cggatcgcct cgcggacata cggcgcgagc gctttgacaa
     5401 agtttatgcg cggcgacagc atcagcacaa tgctttccgt catgacggtg gtccggcaaa
     5461 gcatgatcaa ctgctgcgga atgatcagct tgtgcgtggc gaggagattt atgcccgttt
     5521 ccagcaggac gtcgagacgc agatcctcca tggggagatc atagtagaac acgatcagtt
     5581 cgtgcagttc ccgggccaac tgcttgacat cgacctgaga gccgggttcg gcataggccg
     5641 caagaagctt gctgagccga acggcatcct tcgtcatcac ggcgacgaac atgtccgtca
     5701 tcagttgccg ggtttccgga tcaatcgtcc gcaccatccc gaaatcgaac aaacccaaga
     5761 catggtccgg taaaacgatg atgttgcccg catgcacgtc ggcgttgaat aaaccatgcc
     5821 ggaagatttg cttggccatt aaggagacca gatgcttcga cagtgtgtca cggtcaaccc
     5881 cctgtcggac taactcgtcg agttgatcga tcttgacccc atcgagcttt tccagcacaa
     5941 gcagtttgtc ggtgcacagg tccttataca tgaccgggat ccgaacggtc gggtctcccg
     6001 cgaaaatggt tttgaacttc aaaccattat gcatttcgcg gtggaaatcc atttccatca
     6061 gaatcgtctt atggaactcg ttgaccatgc tgcgcagatt caagcgccgg aactcctcca
     6121 catatcgctc aagcagaggc agaaaccggg ttagaatctc gatgtcggtt gcgacaatgg
     6181 tctggattcc ggggcgctgt atcttgacga caacggattc gccggttgac gccaagcggg
     6241 cctcatgtac ctgcgagagg gaggcggccc cgatcggcac ggtcgaaaac tccgaaaaca
     6301 catcccgcag cgggcacccg tattcggtct cgacgacaca gatagcgtcc tgcgcgggaa
     6361 tggcggcgac attgtcttgc agacgggcaa gctcaagggc gataccgtct gggagaatat
     6421 cgcgccgggt gctcaggatt tgacccaact tgacgaaggt cggccccagc gactcgagcg
     6481 ctttgcgcag attttccccg atacgggcgt tcaagtcagc gggtgcgcca cccgccctga
     6541 cggaccggcg agcctggatt ttggccttca ggcaagccca tccgcatcgc gcgaacacga
     6601 caaaaatctg ccgcatgcgc ttcgcgttcc gatagggacg gatcatcatc agccgagtca
     6661 aacgcataca aatccccatc gcgcgcgagg gtgatgcgcc cccgaagggg caccgctagc
     6721 gcagcgattc tgcctgacat cgctgaacgg gccgcccgcc gccatgccgc cagcgaaatt
     6781 cataacgcga tcaggccttt gttgcggcgc ggggtgcttt catcgccgcc ttcaacccgc
     6841 tgatctcttt gtgaattttg gcgagatcgt ccgccgtcac gagtcctaac tcgcccaaga
     6901 ctttcttcgc cgaatcccga aattgatctc cgactttctt tttgcatgcg gcaccctcgg
     6961 attgcacctc acgcagaaaa gcctgcgact gctgtttgct caactcgcct tttttcacaa
     7021 gcgattcgac tgccgcaacc agtttcttct ggcccgtcac agccaagccc agcacaatct
     7081 cgactgcaca cacgccctct tttttcatat caaacactcc tctgttactg cctatccgtc
     7141 tcggtatgga aaatccggaa aactccggtc ccaacaatga gattatggag ggaacggatg
     7201 aaaagagtca accccgaaaa gtgcgcaaaa atccaacgct ccaacgcggc gggcacattc
     7261 gtttgctgga acttgaagcg cctcaccgtt tctggcaaaa tacgccacca aatggtccga
     7321 gcaggttccg ccatcacgat tgaagccccg gccaagatca acctgaccct ggagatcctc
     7381 ggcaagcggc ctgacgggta tcacgagttg cgcagcatcg tgatgccggt cagcctgtcg
     7441 gatacgttgc gcctgaccac cacctacgcg cagatcgcca tgaccatgac ggtcagtccc
     7501 ggcgtggacc tcacgaagat cggcaccgtg gaccagaacc tcgtggtgcg caccgcccgg
     7561 ctgatgcaat cacgctaccg ggtcaatgcc ggcgcgcgca tccacatcca caaacgcatc
     7621 ccgatcggcg gcggtctggg agggggcagc gccgacgccg caggcgtgat ccgcgggctg
     7681 gaccggctct ggtgcctaaa ccggccgcgc gaggaactca tggcgttggc cggcgagttg
     7741 ggtagcgacg tgccggccct gttccatggc ggagtcgtgc tcatggaggg gcggggcgaa
     7801 cgggtgcggg ccctttttga cagtaccgac ccaatatgcg gcgacggttt ctggctggtg
     7861 atcgccaacc ccggagtcat gtgccccacc tcagaggttt acaagaagtg gagagggggc
     7921 ttgacggcag ggccttcgat tcttcataat atgatttctt cgatccggac cagcaatgtg
     7981 gaaactgcgg cggatgcgct ttataacgga ttgcaggccg gggtatttga aggttacccc
     8041 ggcgtcgctc agacagccgt gaaactgcgt gaggcaggtt gtctgggtgt tttgctttcc
     8101 ggtagcggcg cgtcggtctt cgggttagtc cgggacgaag cacatgggcg ggagatcagc
     8161 cagaggctgg ataacgacgt gtggcacgta ttggcgcgca ccttgcccgg tggtgtaatg
     8221 gcagcacaag agactttgac tctctcagag gtggttcgag tccaccccgg gcaaccagtt
     8281 ttgaggaaag aatgatcgta ttttccggaa cagcgaatcg gccgttggcc gaaaagataa
     8341 tgacctatct ggaggggcgg ctgggggata tccacatccg gcgtttcccc gacggcgaga
     8401 cgcacgtgaa gatcgtggac aacgtgcgcg gcatggatgt gttcatcgtc cagcccatct
     8461 gcgggcagcc caacgaaatg ctgatggagc tgttgatcat gatcgacgcc gcgcgccggg
     8521 gttcggcggc gcgcatcacc gccgtgctcc cctattacgg ctatgcgcgc caagaccgca
     8581 aagaccagcc gcgcgtgccg atcaccgcga aactcgtggc caacctgctg gtggccgctg
     8641 gtgccaatcg cgtgctgacc atggacctgc acgcccagca aatccaagga ttttttgata
     8701 ttcccgtcga ccacctgcac gccgcgccgg taatggtgaa atacctgcgc gaaatcgagc
     8761 tcaaaaagcc gctggtcgtg tcgcccgata ccggaggggc aaaagcggct tacgcttatt
     8821 cacaaatgct gggcaccggc ctggcgattg tggcgaagca gaggctggga gacgcggaag
     8881 tggacgcctt tagcgtcgtg ggtgacgtga aagggtgtga cgtgatcatg gttgatgaca
     8941 tgaccacgac ctgcggcacg ctgtgtgcgg cagcgaaaat cctgaaggag cagggggccg
     9001 cgtccatccg ggcggcggtg acccacatgc ccctgacgga taaaggtgta cagcggcttc
     9061 aagccgacac cgggctgacg gaactggtgg tcacggacac ggtccccctg cgggaaggtc
     9121 tggactccgc cgccataccg gtcaagctca cggtgctgtc tgtcgccgac ctgctgggcg
     9181 aagcgattcg caggatccac aatgaccagt ccgtcagctc gcttttcagg tattgacggg
     9241 cttttttcac actgacagag gaaatcatgg cgaacgaaaa gatcagaatg aaagccgagt
     9301 tgcgcacgga gcagggttcc ggcgcggccg gccgtttacg ccgtgccggg agcgttcccg
     9361 gtgcggtgaa ccggatcgag ggcggcacga cgctggtcaa gattgatgcg catgcgttta
     9421 aaaccatgct gcgtcatcat ggccgggagc agatgctggt cacactcgag atggacgaca
     9481 aggaagtgtc cgcgctgatg cgcgaggtgc agtacgatgc gatcaccggg tatccgatcc
     9541 atgtggattt cggtgaagtc tccctgacgg agaagatccg cgtggcgctc ccgctgcaac
     9601 tgacgggcga gcccgagggc gtcaagctgg gcggcggcgt tctgcagcag atgctccgca
     9661 cgatcgacgt cgagtgcctg cctaccgatt tggtcgagca tttcgaggtg gatgtctccg
     9721 gcataaaact ggggcagtcg cttttcgtgc gcgacctgaa actcggcgac gcctacacgc
     9781 ttttcaccag tagggacggt gtggtggcca cagttgccgc gccggaggaa gaggaggagg
     9841 cgacggccgc gacgccggaa gctgctgcgg ccgcgacgcc ggaagtgatc accaagggca
     9901 agaaggagga ggatggcgag gcctccgctc cggcggccaa gaagtaatcc ggaggacacg
     9961 accgcgtgaa aatgattgcg ggcttgggta accccggcga gatgtacgcg aacacgccgc
    10021 acaatgtcgg tttcgacgtt gtcgacctgt tggctgaacg gctcggttgc ggatggaaac
    10081 gcagtgcccg gtttcacgcc ttcgtctcgc ggacccgttg cgcggaggag ccgatcctgc
    10141 tggtcaaacc tcagactttt atgaatctga gtggaaccag cgtggttccg ttgcttcgct
    10201 actacggggg tgagccggat gacctgattg tggtgctgga tgacgctgac ttgccgttgg
    10261 gcatgttgcg catccgggcg gcaggaggca ccggaggaca ccgcggactg acttccgtga
    10321 ttgaggcgct gggaagcgac gccttcacgc gggtccgtct gggtgtggga cgcgaaggac
    10381 gcggaagtct ggccgatcat gtgctgggca agtttgacga agcccgccaa gagaaggccc
    10441 gggcggttgt cgaagccgcc gccgacgcgg tgcagtgtct gaccgaaaag ggattgaacg
    10501 aagcgatgaa ccggtacaac ggctggcggg cggatgcgcc gctgccgccg gacgaccgga
    10561 agtgagatgg gaaaccaatg atgaagaaat acgaaggctt gtatatcttc gccggctcgg
    10621 cgaaagatga cgtgctggat aagcaggtcg aaaaagtgct cggagagatc acgcgcctgt
    10681 ctggcaacgt gatcagtacg gatgtgctgg gcaaacgcac ctttgcccgc ccgatgaaca
    10741 aacgcgaaaa cggcgtgtac gtgaagatcc gttttgagct ggacccccag cacgtcacgg
    10801 atctgaaaaa ccgctaccgg ttgcttgaag acctcttccg cgtgcagatt ctggtggtcg
    10861 acgaacgccg cgagagcgtg ctcgcccagc aggaggaggc gcacaaggcc cgtgccgcag
    10921 ctcgcgcagc ggcagcggaa gcggaggcag aagcggcagc ggaagcagaa gcggcagcgg
    10981 aagcggcagc ggaagctgca gaggaagagg aagaagaggt ggaatctcaa gacgagcgcg
    11041 atgcgtaaac gcgacgcgcg gcggaaagga cggtgagaca tggcatcctt caacaaagtc
    11101 atcctgatgg gcaacctcac gcgggacccc gaactgcggc agactccgac cggcgcgaaa
    11161 gtcgccgaac tgcgtctagc cgtttcagag acctggcgcg acaaaagcgg acagtccaaa
    11221 gaggtgacct gttttgtgga tgtcgtggtc tgggaaaaac tggcggaact ctgccaccgg
    11281 tacctctcca aaggcaggcc ggtgctcgtc gaagggcgct tgcagttgga cgagtggaaa
    11341 aatcagcaag gggaaacccg aagcaaattg cgtgtgcgcg ccgatacggt taaattcgtc
    11401 ggcccaccgc ccgcccgttc cggagagacg ccaaccgcgg cacagccagc agcccctgac
    11461 gcgtccgtac ccgttgcgga ccgtacggat tacaacgtcc cggacgacct caccgaagat
    11521 gatgagcaca tcccgttcta aaccacacga gcgattaacc aaaggatcat gtctatggcc
    11581 atgcaaagaa aatcaacttc tcaccgcgga aaccgcgaaa gccgtgaccg cgacagcggc
    11641 ccggcgcgca agcgcgcgcg gcttctggac ccgaacgaca tcatcgatgt gaacgatgtc
    11701 gaattcctgc gccggttcac caccgaatac ggcaaaatcc tgcctgcacg cctgaccggc
    11761 gtgacagctg cacagcaacg ccagatcaaa ctgggcgtgc ggcgcgcgcg caacatgggc
    11821 ttactggcct agcccggaaa ggaacgttac attatgtcaa ctgaactgtt actgctttcc
    11881 gacatccctg acctcggtca ggccggtgat gtcgttaaag tggcgcccgg gtacgcgcgc
    11941 aattatctgt tgccgaaaga tctggccgcc ccggtttccc cccaggctct gcgccgcttg
    12001 gaaaagttgc gtaaggagcg tgaggaacag acacgcctcc gactggccga ggcgaacgcc
    12061 aaggccgcca aactgacgaa cgcctccgtc accctccgcg ccaaaaccgt ggacggcacg
    12121 cgtctgtacg gctcgatttc tgccgccgac atcgcggccg ccatcgagac ggagaacaag
    12181 gtgactgtgg acaagtcgca gatagaactg cccgagccga tcaaagagac cggcaccttt
    12241 gacatttcca tcatcctgca tgacgatgtg aagcctgtca tcaaggtctg ggtagtcgaa
    12301 gactgaggcc tccgcaacac acaaaaagga gtcaggcgtg aggcatcagg gatacccacc
    12361 ggttccgtga gcctcacgtc tgcttttttc gatcatgtcc cccaccccgc ccaccccaaa
    12421 gccgtcctca cgtatcccgc cccatagcat ggaggcggag cgcggtgtgc tcggctccat
    12481 tctgctcgat gcggccacgg acaaccgtgt gcttgatctg tgcatcgaga gcgggatcac
    12541 ggcggattcg tttttctccc tccccaaccg gcttttgttc gaaacgctgt ccgagatggc
    12601 ccgcgccagc cagatcatcg atccgctcac cctcacggaa cgcttgcgcg cacttaaccg
    12661 gctggaagag atcggcggcg ccgttgccat ccagacgctg atcgacgaaa ctcccaccgc
    12721 cgcgcatgcc gagtattaca tcaacatcct gcgacagaaa catctgttgc gcgcggtcat
    12781 cacctgtgcc cgcgatgccg aacgccgctg tttcgacgag gccatcagcg cggacctgat
    12841 cctgagcgaa gtcgagcaga agttcctctc cattgccgac cgtcgcgatg gggccggact
    12901 cacctggccg caggccatca ccaatacgat ggggcatatc gaccggcttt tctcgctcgg
    12961 tcccggtgcc gtggccgggc tccccaccgg cctcgccaac ctcgaccgta aactcaaagg
    13021 gctgaggaat ggcgagatga tcgtgctcgc cgcgcgcccc tccatgggca aaacctcact
    13081 ggccatgaac attgccgagc gtgtcgcttt gggcaaagac atgaacgggc gcgccatgaa
    13141 gggcgaccac aaccgccccc atcccgtcgc ggtgttctcg cttgagatgt ccaccgagtc
    13201 ccttgccatg cgtatgctct gtggcctcgc ggaagtgccg agcttcaaaa tcgaccaggg
    13261 gttggtaaac ccaaaaaaga ttaaccggca actgactgat gcggcatcca aactcgctaa
    13321 agcgccgatt tatgttgacg acaccggcgg tcttgacgtc atggacatgc gcgcccgcgc
    13381 ccgtcgcatg aagaagcgtt acaaggtcga gttgattgtg atcgactacc tccagctctg
    13441 caactgccgc gaattcgccc ggcagggccg ccagattgaa acctcacaga tctccggcca
    13501 gatcaaagca atggccaaag agttaaacct gccggtcatc gtgctcagtc agctcagccg
    13561 cgccccggaa cagcgcggcg ataaaacaaa caagcccaaa ctctccgacc tgcgtgactc
    13621 cggtgccatc gaacaggatg ccgacgtcgt cctgctcctg cgtcgtccgt gtaaagcatc
    13681 cggagatccg gagtttgatg acaagctgct cgccattgta gacgtggcca aacaccgtaa
    13741 cggcccgacg ggcgaggtgc gcctgaactt cgaggacacc ctaacccgtt tcagcgaccg
    13801 caccgaccag atcacggagg atgaccggtt cgaacccgac ccggactcct gacggacccg
    13861 cctgattctc cgccgcagcg gttgcgcaag gacggtcgcg cgcgaacaga cccgcatcct
    13921 cttcccgaag atacgccagc gcatccgctt cagcgcggcg cggcgcgtct gcggcacacc
    13981 atctcgcgcg cctgctccag cgtggcaagc tgtccctcaa gctgcgcatc gtacagttcc
    14041 cgcaggagct tccccacctg cggtccggac ggcacccccc acgcgatcag atcccggccg
    14101 cgcaccagcg gttcgggcag tacaggttcg gaaacgaaag cggcgtaggc ctccacaagt
    14161 tgccgccaag gagtgagatc gccgttgctg tgctgtatgt ccagccgcat caactccaac
    14221 atccgcggaa acgtgggctc tcccatgaag cgccgcaagg tcgcgcgccg catcttgccg
    14281 gactcgaccc cccgcatgtg ccccgccacc aacgcgcgaa cctcggcgat ccgcgcagac
    14341 ggctgacgca accgggtgag aagttcttca gccattgccg cgccgaccgg cgcatggcag
    14401 gggaaacgga tgcggaccgc accggttgca ggatcggttt cctcacggca cgtcatcggt
    14461 ttgccgagat catggaggag caccgaccaa gccaaggtca gatcgcgcgg cggcggcagg
    14521 gcatccaaca tcagacaggt atgggtccag acatccccct cgggatgaaa tcggggaggc
    14581 tgagccgttc cgtacaggcg ggccacttcg ggcaggatcg gtgacagcag acccgtctcc
    14641 cgcatcaaat tcagagccgt tgagggcaag ggcgcctcgc acaatatgcg gttcaactca
    14701 tgggccacac gctcgggact gacctccgtg atgcggggcg cccagacctc cagggcctcg
    14761 cgcgtacgcg gctccaccgt gaactcgagt acggccgcga atcgcaccgc acgcaacata
    14821 cgcaggcggt cctcacggaa tcgcgccgca ggatcaccga tcgcccggat cacctgcgcc
    14881 tgcaaatcag tcaacccgcc cacataatcg gtcacgtcgc ccgtagcggg atcgcagaac
    14941 agaccgttca ctgtaaagtc acggcgcaac gcatcgcctt ccgcgttggt gaacgcgatc
    15001 tgctcaggat gccgaccatc gcggtacgga ccatcggtcc ggaaggtggc cacatcaaag
    15061 gagaagccgt tgcgccgcgc caccaccaca ccgaacgcct tgccgaccgc caccgtctcc
    15121 ccgggaaaca acgcctccac ttgatccggt gtcgcggcgg tcgccagatc gatatcctga
    15181 actggccgac ccaacagggc atcccgcacg catccgcctg cgaaaaaagt ctcgtatccg
    15241 gcgcggttca gatccaaggc cagcgcccgc gcggcctccg cgcgcgggat atccccggcc
    15301 cggaaccgca cctgcacacc gttgccgccc atcactcacc ccccgcccgg atacggaaaa
    15361 agagggtgtc ggccaagccg ggcatcgcca tcccggcgcg gaaacgatga accccgttgg
    15421 tctcgccatc gcgaaccggc gcgacagaca tccctcccca cccgtctgtc cgcagcaatg
    15481 cgttggtctg cacctgatac ggaatccccc gctccccaag aaaatcaatg cagaacgcat
    15541 cgttcgtcac ggcaaacagg agaatgtccg gcgtcagcca aggttgcggc acatcaccag
    15601 tccagatcac atcatccacc caccccgcat cgctgctgct ggcttcagca ccgttcttac
    15661 gatactccca ccgcaacgta tgccaggttc cggtaccgct caccgtattc gtcaccgcag
    15721 tccacgggga cttggtgccg gaaatctcca tgaccggagt cccgtccacg agaaaccgca
    15781 gaaaatcggc accgggctca ctcgacacac gccaccagaa gctcaccgtg ccgctgcccc
    15841 aaacctgaag ttcgaaagcc gtgaagccgt tatcgggaac cacccccgga cccccaagtt
    15901 taaccgccgg cgtaccggag ggcgcgccgg acatcgcgtc accggtaaga ccgtcaacca
    15961 ctccttcaca cgtgaatgcg ggcccgtaac acccgagcag acgcgccacg gaatcgcgat
    16021 acgaggcaat cagcgtcacc gcctgcgacg gcatggtgag caccgtgctc ggtgccgtcg
    16081 cattcgccag tgccgaagtc gcgtcaccat tccatccggc gaactcctga taaaggggcg
    16141 cgggatctgc cgccaccatc acggccgagc cctctgcata ccacccgctt ccagagccgc
    16201 aaacaaccgt gaggggcacc cgataggtgg ccgccacaca caatgcggaa tccgtggtcg
    16261 ttacagtggt ggtcggcgcg aaccgatccg ccaccgttgc cacgtcaccg gtccagcaat
    16321 caaactgttt tccttccggc acctccgcgc cgatcaccac ctgcgagccg tagggataca
    16381 caccggaacc actgccgtgg atcaccgtga gcggataacc cgtagcctgg tacgacgcgg
    16441 tcaaaaccat ccccgttccg ctcattgtca cggtcgtcga tgctgccagc acgtccgcca
    16501 cactgttggt gtcaccggtc caccggtcga acacatgata cgcgggcggc gaatcggcct
    16561 gaatcgccac aaccgtgccg ttgctgtacg cgcccccgcc gctgccgtgg atcaccgtga
    16621 gcggatggaa cgggacccat cgcaaggcgt caacccagcc gcagtcctgc ccgacataat
    16681 caccttgcgg atccttttca tactcccacc ggaagaggtg cggcgtctct tgaccgccct
    16741 ccaccagaat cgacatctcc tcccagttgt tcatgatgcc tgagatttga tggatcgacc
    16801 ccgtgccggc ctcgtagaaa cgcaacacat cccagtcagc ctcgctcgaa gtgcgccagc
    16861 agaacgagag caggcccgga ccggtcaccg accactcgat gcccgccatc tggccgtccg
    16921 caatgatccc tttcccgccc agacagacag aagttccgcc gtcaccgcag tggttggtgg
    16981 aaacttgaac ggcattcgta aacccgatcg gcacggcatc ggacgcgcca ccaacaacga
    17041 tcagcgcatc cgcgcgccag tgcgcgtaca cggtatggtt ggtaaccgta cgcatcaccg
    17101 tatcttcggt cactggcgtt ccggcaccgc cgggtccgga ccgccatccc tcccacacgt
    17161 aacccgtccg ttccggcacg ggcaaatccc cgtaaacccc gccaagatcc accacagccg
    17221 tggcgggctc gaccgtcccg cccaaggcgt cgaacgtgac cgtgcaggcc cgggcgaacc
    17281 cccgcaaccg cggatacccc tccccttcca cgatctgcca gtccccgatg aaatcccagc
    17341 ccgaataggt cacggcctga cgcatggccg atgtggtttg cggcacgccc ccggctgaac
    17401 cgctctgtcc gctgacctcg gtatcccaaa acgaacctgc ggtgatccca tccccccagc
    17461 cgatcagccc gcccacacca ctggatgtgc ccgtgaccac gcaagccgca tacgtctggg
    17521 tgactgagcc cccgtcggca tgcagtccga tcagcccgcc cacattcgac tggcccgcca
    17581 cgctccccac ggcatacgcg cggttcaccg taccctcgcg attctcgccg atgaggccgc
    17641 cgatcccgtt gtacgtgccg ttcacctgcg cctccgcgaa acaccgctcc accgtaccgc
    17701 ccgcattgta tcccacgagc gcgccaagcc ccccgttccc gcacacctcg gcataggtca
    17761 acccgagatt ccgaatcacc gcacccggtc cgagaacgcc gaacaacccc gcataatcct
    17821 cctcgggacg atccaccttc agccgggcga tcacattgga ccgaccgtcg aaactgccgg
    17881 tgaacggcgt tgtcatgctg ccgatcggac cgaacccgtc cggccatccc gccgtctctc
    17941 cggcatacag catcccggtc agccaatagc ggccattcag cgtatagtcc ggatcgcggc
    18001 cgatccgctg cagttcctga accgatccga taccccgcgc cgaacggaag atcgcggtca
    18061 ggctgacatg cccggccatg gtcacctgcg tcaccggatt cgtcggctgg gccacgccgc
    18121 ccgaccagcg gacgaattcg ctgacgcccg ccaccggctg cgcggtcacc gtcacattcg
    18181 tcccgggcgt gtaagaggag agcaacgggg tgacgaatac gttgcccggg ccgcgcgacg
    18241 caaccgtcaa cacaaactcg ttcccgttga ccgccagccc gcgcagatag gggtagctca
    18301 cactctccac gataccccac acctgactga aatcccatcc ggaaaaggtc acggcctgac
    18361 gcatctccgc cgaagttttg cctgtccccc cgccccatga ggaggattgg ccgcttgcct
    18421 gcgtgtccca ataggctccg gagacaacac tgttgaaccc gctcccgctg cccacaagac
    18481 cgcccgtgtc ttccgtgccg ctcacccgtg cgatcgcata actctctcca atcgtccccc
    18541 cctcatacag gtacccgacg agtccgccag cccccacgtc ggccacaacg cttcccgacg
    18601 cggacgaacg ctcgatcccg cccaaatcca caaagccggc cactccgccc acggtttcca
    18661 aaccgaccac atccccagaa accgaacact cccggatcag gccctcagac agatacccgg
    18721 ccacaccacc gacatcaacc agccccgcga ccgtgatccc cgtcagacag agattgctga
    18781 ccaccccgcc cgtcccaatc cggccgaaca ggccgacatc actctcgccg ggacggttga
    18841 gggtcaaccc ggtgatccta tggccgttgc cgttgaacac accggtgaac ccccactccg
    18901 catcccggcc gatcggcaca aaccccgcgc ccccgttcca atccgccgtg gccgaggcgt
    18961 ccagattcgt catcaacgta tagacagcat cgggctccca cccgtcgatg ccggatccca
    19021 ccttctgaag gtcatccaga gaccagacct ccagacccgc cgcagagagc gcacctgcca
    19081 ggcataggca acccgcaagg cggcgcactg tgcgcgccgc attcgcttgc cctgccgtgt
    19141 atcgcggtgg ctgattactg agtcctgttt ttttcatgcg gccttttttc ccacaacccg
    19201 atccgttagc tcaggattgt acccgtacac acgtcgaatt tcaacctctg acacaagcag
    19261 gccatcttcc cgattcccct ttcggctttg acaaacgaaa cgtcgaaggg gtacactgtc
    19321 gcaccatgcc tcatcgaact ttgagtttgc agcaagccgc gcgccacatc cggattccgg
    19381 aacgcgagct gttccatttg gttcagcgcg gagagatccc gtttctgaga cacggcgacg
    19441 aagtggtctt cgagcaccgt cgactcgacg attgggcgca acggcgcatc ctcggcctgc
    19501 cgacaaaatc cctgacccag catcaccgcg aaaccacggc ggaacgcacg ccccatagca
    19561 aagacgatgc cttggtggcg cgactcttcc gtcccgaatg gatcgacccc gccctgccct
    19621 cgcggaccaa gcccgcagtc gtgcgggaca tggtcgcgtt ggcggtctcg accggactgc
    19681 tgtatgacga cgcggccttt caacgcgagg tcgaagagcg ggaggccgcc tgttcgaccg
    19741 ccatcggggg cggcgcggcc ctgttgcatg cgcggtatca cgaccccttc tgggcttcgg
    19801 attcgtttat cgtgctcggc aagacgcagc agaacatttt tttcggcgcg caggacggcg
    19861 gcgagacgga tttgtttttc ctgatctgct gtacggatga cgccttgcat ctgcatgcac
    19921 tggcacggtt atgtatgctc gcacacggca gcctgctcct ctccgacctg cgcgccaccg
    19981 accgagccga cgagatgcat caaatcctgc tgcgcgccga aagcgaactc ctgcgcaccc
    20041 tgtgagccgg gtcatttcat ctcggactca tacggaatcg ggataccgta ctgctggttc
    20101 actttgagca tttcggccag cacctgctgc cgcggttcgg ccgcatagac gctgaccaga
    20161 tggtacagct tctgatacgc ccaacgctgc cttaccgccg ccgtgccgag tttggcgtgt
    20221 cgcgacaggc tgagccggaa aatcgaatcg taccccttgt cagcagccag gccgcggatc
    20281 tgaaacacca gttcctccgt ggtgtcggga cagaccccgt gcaactggat ctgacgatcc
    20341 ttgtagagag gcgtcgtgcg tttcgggtag acctcgctct gcgacacact gtcgaacgtc
    20401 acctggatat cgctcatcac cggataacgc agcccagtgt acgctccggc catcgagtcc
    20461 ggaatatccc accgattacc ggtgagcaca accgacccgc cccgattccc gtacgtcaac
    20521 atatcgagca gatagacatt ggctttgctc tgtgtcccgt acatgttgac ggagaccacc
    20581 ccctgattca gcttcgagaa ctcgccaatg atcttggcgc tctcggtcat gccaaaggtc
    20641 ggcttgccgt ccgtcacaat gaaggcgatc atcgggcgtt tcggatcacg cggcagtttc
    20701 atgagtgtgc gcaacgaacc gaacacgtcc gtctggccga acgcgcgcat ggccgcgaca
    20761 aaagttgagg ctttcttgag gtgggcctgg gtcaccggaa cccagtccgg aaaacacatt
    20821 tcggtggtgt cccggaacgc gaccacgtta aagcggtctc cggggttcat cgtttccagc
    20881 gcggccgtca tcgcgcggcg gcagaagacc atgcgctcct cggacatcga ggctgagaca
    20941 tcctgaaccc agacgatatc cttgtcgagc gtgggcaccg tgcggtcgcg gcgcggctcg
    21001 attccgatcc ggaaatagat gcgtccggga tccgccgggt cccggtaggt ttccaagccc
    21061 acagccaaca gatcgtcgat cggctgatag tcggccagct cctgcaacgc cgcgatctgg
    21121 gcctgggcct cacgcgcctt gcgctcttcc tcgctgactt cagtagcggc gatctgttgc
    21181 gatacggcgg ccagttcacg ttcgatccgt ttctgctgct ccgaggtgct cgccccgtcc
    21241 ggtccagccg ttgccgagcc ggtgtccgct gccgagccgg tggtcgggct tttccctgct
    21301 ttttcgccgg gctgcatcgc aaaccgggac tccgtggctg cggtggagac cggcgcgggc
    21361 gtggccggcg cctccggcgc gggaaccttg aaggtacccg cgctgatttc ggtatcaaac
    21421 acctccgccg cctcgaatgg cttgggcggc tcagggtcgt tgccaaaacg gcgtcccacg
    21481 aggtcgatcg acggcacgat gtccggcgcg tcgggaaccc gttcaatcat ggggatctcg
    21541 cggcgcggca gagtcgcgac ctcatccacc acggtccggt cataaatctg ggcgatctcc
    21601 tgacggggca tccagggggt ggtgtcgatc tggggcgagg ccgcctcctt gagcgcgggc
    21661 acacccggtt gaagcgcttc ccgcggaatc ggcgggggcg cggtgagaac gggcgcggcc
    21721 gactgactga gcgcctccac ctgctcggaa acctcgatcg ggccgcggct gggcgtatcg
    21781 cgctcacccg gcttcttccg accgatatac atggggtccg cgcgcatggt ttcgacacgc
    21841 atgggcggca cgcggtcgcc cgtgaaccat tcgcggcccc gttcgcgcaa actggagatg
    21901 ccggaaatcg tccaatcgga gaaatagacc atcaagacca catggatcac cgcggacaac
    21961 aggaccgcga cacaggccag cagcagatga cggtcatggg attgcggatg cggtctgaat
    22021 ccgtatggtg tcatgagcct gccttcctct cgcctgcggc cgcggtcgac ggaaccggat
    22081 tcgccaacgg cgaggcggga tagcgggtgc gcaactcctc acgcaacgcc gcagcctccc
    22141 gggcccgccc ggacttatcc agcaagaccg ccgcctgatg caacgactgt ggcacgagtg
    22201 tgggatcatc gaacaaaatc ccgacactga tatgatagcg caacgcggcc tctgaatctc
    22261 ccttgcgctc ggcgttgcgc gccaatccgg cataggcatg cgcccgcagc cccaacaggt
    22321 cgggcgtggc cgcgcgcgac gccgcgtcgg tcagatgttt ctccgcgtcg tcgaaccgcc
    22381 ccgcttcgct caacaactcg ccgagccgga gagcggactc cgcgccgtat gcggtagaag
    22441 cgcccgtggc cagcgcctcg gtaaaggcgt gaagagccgg atcgcgctcg cccttggcgc
    22501 ggtgcacgcg gcccagcagg gtccacccgg tctgcgtcca ccccttatcc accttcaact
    22561 cgaccagtcg ggtcgcggcc tgaatcgccg cagccggctg tttctgctcc agttggaact
    22621 cggcaagcca cgccagccgc tcggcaccga gtttttcggc gatcggcgcg tccagcagtt
    22681 tctggaaaaa ggaggcggcc tcgggtttac gcccggtctt ctggagcagc atgcccagtt
    22741 ccagcatcga ctcccgctca aagtccttgg tcggagacgc cgcgagacag gcacggaaca
    22801 gcttctccgc ctcggcgtct tcgccgacct gccggtaggc ggaggcgcgc cagaacagaa
    22861 cctcggcctt gcgggcgtca ttcggaaaac ggcgcatgcg ctcatccagt gtggcggccg
    22921 cggcgcgaac atttttcgcg cgcaactcct ccatcgcctt ctgatagagc gtctccgcga
    22981 cctcggcgca ctcgggatac cgggtcagca gcgcggtcca gtcccgcaac gccgcatcgc
    23041 tctgtccgag acgagacagg cagacaccgg aggcatagag ggctcgggag gccagcggat
    23101 catccgggaa acgctggacg acctgcaagt accaggacga agccgactcc catgcctcct
    23161 gtttctgcag cagccacccc agccggtaga gggcatcttt gacaagcggg ctttgcgggc
    23221 acttgtccac cagcaggcgg cagtactgca cggccgcatc ataccgctgc aacgctaacg
    23281 ccgcctcggc gttcatccaa tagacgtggt cggcgtgcaa gtcgaccggc cgtgtgttct
    23341 ccgaggtcat cttcaacacc gcctcatacg cgccgtcatg atacagcgtc gtcagcttct
    23401 catacccggc ctgtccggcc agccgccctg ttgggaaggc ttcgagttgt tgcgaataga
    23461 ccttcacggc ctcggcacgc cgttcgagtt ggcgcaggca attcgcccgg aggtagagca
    23521 cctcttcgcg cgccggatgt ccggcgtcgc cgatgatcag atcgagactc tcaaaggctt
    23581 cggcataacg ccccgtgtaa tagttcgccc agccggcgga cagcgcgctc tcgcgtatgc
    23641 gcggcgaagc gggatacttc gtcttcaacg tcttgaacag gttggcgctc tcctcgtgcc
    23701 gctggtccaa cagcgcggtc tgggccgcga agaacagcgc ctcctccgcc actttgggat
    23761 ctcgggcctt gtgcgccaaa tccagataga tgcccatggc acgacgccga tcctcgggct
    23821 tgccggtctt accgagcagc cacgcgatac gcaatcccgc atacatgccg tagtcggtct
    23881 gcgcgtactc ccggcttaac tgttcatagc gcgccagcgc ctccgcaggt ttgtcgagct
    23941 tttcaagcgt ctcaccaaga tggtacagcg cggccgaccg cacttccggc ggcatgcccg
    24001 tcgcggtcag tttttcaaac agcgcggccg caacgttcag ttccgcaccc ccggccctga
    24061 gcagcagcag cgcccgctgc agatcggcgc gcgcgacatt cggactgtcg ggaaaacttt
    24121 cggagagttg cttgtaggtc gcggccgcct ctgccgcgcg gcctgtgcga cggtaacact
    24181 cggccagacg gaacagcaca ttgtcgcgat ccggcacttg cggcgtttcg gccaaggaca
    24241 cgtattcgcg cgcggccaga tcaaacaggc tgcgccggaa gagcccgtcc gccagttgca
    24301 gacggtcccg catctccacc gggtccgccg cacgcacgcc gcacacggcg agcagcagca
    24361 ttccggcgct aatctgtgcc cggcatatcc tcatggcgtc tccgtggcgg caggcttctc
    24421 ttcgctcgtg gccatcgaga agttccagat gtccgccttg cggcaggtat ccaccacctt
    24481 gatcaggtcc tcgtatcggg tctctttatc cgcccgcagc accaccggtt ggcccgggaa
    24541 gtagcgcgcg agacggtcca gccgggcgcg gagcgcatcc agcgtcagat cctgctggtt
    24601 gaccgacacg cggccgtcac gggtgatgtt gatgatgatc tcacccggca accggtccgg
    24661 cactttgccg ctctgcgccg acggcagcgt gatgttgatc tcatactccc actgcgagaa
    24721 gacggacgtc gtgatgaaga aaaaaagcat caggaaaatg atgtccatca tggacgtcat
    24781 ctgaaaactt ggaagcacac ccctgctttt ctttcggaag ttcatgcctt gtcaatcctg
    24841 tcccgtaaac ggggctttca tccccatgcc gatgagggcg gtcagcacct cggacgacgc
    24901 cgcctcgagg ttggcgatct ggcggctcgc ccgccgccgg aaccaagcgt aaaacaccat
    24961 acaaggaatg gcgacgatca acccgaagac tgttgtcacg atcgcctgcg acacgccggc
    25021 cgccaatact accggcttgg cgctcgcgat gtcgctcgcg accgagccga aagccgtgag
    25081 cataccgagc accgttccga gcaggccgag caacggcgcg atcgtggcca catccaacag
    25141 gaattccgtc tgcccctgaa tcgcgtcagc ctgacgcgca ccctcggcct cggccgtgct
    25201 ccggagcagc gcaacatcgc actgcggcac attgcgcaga cagtccagaa ccgccaacac
    25261 caccgacgcg agcggacagg gatgacgatc gcataaacgg cgcacctccg tcagatcgtt
    25321 ggcgcgcacc cgcgccacca gatcgatgag cagttcgcgc ggcgtgatcg ccccggaacg
    25381 caacacggtc atgaagtaga acaccatggc cagcgcgaag accgaaatcg cggccagcac
    25441 ccacatgacc catccgccat attcccacgc ctgtttcagc gacatcccgt atccgttgat
    25501 cagggccgga acagccgccg cgctttccgc cgcctcttgc gcccacgcgc acgccagcga
    25561 ccccgtgaag gccaaaaccc ccgtgaccgt ttttctgttc accatcctac ccatcccttc
    25621 tcctgtttca tcccacaccg gatcttccga ttccggcaca tcatccgagc gcgtccgccg
    25681 cgccgcccaa cagggcactg aagatcgccc gtagccgcgt gcgccgcaca cgcacccaac
    25741 gccgcacatc gcgcacacgc gcacggtatc cgccaaaatc cagtcccgcc gtggctccca
    25801 gatgcgtctt cgccgccaac gcccggtccg gatccgcttc ctgcaacgtt tccagatggg
    25861 accggacatg ctgatacgcg tcgcggaacg gcatgccgcc cgccaccagt tccaacgcgc
    25921 ggtcggtcgc gaacacgtct ggcgtgaacc ccgcgcgcaa ccgctcggga cgaaccgtca
    25981 gcgtacccac catcagccgc atgatgcgca acgcggcgcg tgtgaccgtc aacccctcca
    26041 tgtacaacgc cttggtctcc tgcaaatcac ggttgtagcc gcccggcaac gccttcagaa
    26101 tcatgttcgc cgcactgccg tatccgagca ccaccgccgc cttggcgcgg atcagttcca
    26161 gcacatccgg attgttcttc tgcggcatga tgctgctgcc cgtgcagagt tccggcggca
    26221 acgtgaaata accgaactcc ggcatggaga agaggatcag atcctccgcc atccgggaga
    26281 gcgaaagcat caactggccg catgccgcca gcaaaaccgc ctcgttcttg ccgcgcgcgt
    26341 tgctggcgtg aaagacattg tggtgcggcg ccgaaaagcc cagcaaccgc gcggtgagtt
    26401 cgcgatccac cggcaacggc accccatacc ctgcggccga gcccagcgga cagcggtcat
    26461 tgtacgtgta ggcggcctcg accatcagca gatcatccaa cagggtctcg gcataagccg
    26521 tggcccagag ccccaccgaa ctgggcatcg caggctgcat gtgggtccgc cccaccatcg
    26581 ggaccttcgc atgacggcaa ccgaaacgga tcaactcccc cgccacttcc gcaacctctt
    26641 ccgtcagccc caacaactgg tcgcgcacat gcagccggat atccaccgcc acttgatcgt
    26701 tgcggctccg ccccgtgtgg atcttgcgcc ccaattcgcc taaacgctca gtgagacggc
    26761 gctcgacagc cagatgaaca tcctgatccg cctgcgtgat acggaactcg ccggcccgga
    26821 tttcgcggat cacttccgcc agagccgcta tcacccgatt acgttccgct gtccccaaca
    26881 cggcgggacg aacgcgcatc cgggaaagca tggtgacgtg cgccgccgtg cccaaacagt
    26941 cccataccgc cagatcgaga tccagcaccg gatccttccc gaccgtgaac gctaaaacgt
    27001 caggatgtat cttgcccaca atcgtcttct ggcttgccgt ctcttttttc atgtgccgca
    27061 acacctttat tcgcgcactc tccgcggccc tgctcccgta gcccgtccgg cgtccgcgcg
    27121 ctgcaaaacc attccgcgcg cggtccggag ttccaactct ctgcgcatcc cctccgcctc
    27181 catcaatcgc ctcaagagtt cgccctgcgc ctcaccagaa cacactcctt gaagaaaaga
    27241 ggcatacgcc tccgaaacag caagaagcat accatcccga ccaacggcag ccatctcaat
    27301 cagacgggcc tgaactgcgg cgcggcgctg aacctccggc tcagcggaac gcatcgccgc
    27361 ctcacgaaaa gtcaggtaag gccgttcata accccaagtt gtaccataaa acgcggggta
    27421 gagcaataag cgcgaccgga accgccgaac aatgcctgaa ctcgtaagcc cgggcttgac
    27481 taccgtgcgc cccaaggccg tgaaccagag gtccaacgcg gcgtcgaaag caagagggtc
    27541 ctctgttccg gtcagaatcc gcgccaccgc atcaggcctc cactcctgtc cctctgccgc
    27601 cgcgcacaac aacttctcaa agggcaggcc atccggacgc cgctcgagca gccaactcgc
    27661 cagcaccgcc gccacagccg gttcgcgcga tgcggcgaca ctctccatcg caaacagcgc
    27721 cgccgccgga ggcaggcagg cccgcgacca caacagcagc gtgcggtcaa tgtcctgctg
    27781 ccgcgtctcc cggccggtgt ggcgcaccac gccgtcgacc agccagagcg gcacgtcgcg
    27841 catcacgcct tcgcgtccgc ccgcctccac catccatgcc ctcaacagcg ccagcgagat
    27901 cgcgcggcgg aatcgtccca gatccgccgc ctcgggatcg ggcagctcga tccgctcgaa
    27961 cacaccgccc ccgggctccc gcacgcgcga ccccaacacc cgcgtgtcgc cgtcacggcg
    28021 gttgccgatc acgatttcca acggacacga gggggtcacc aacttgagcc gtgtggcccg
    28081 cgccagctcg tcgcgcgtcc ggtccacaaa caccaatacc ggggcgcggt aggaccccat
    28141 cgcaaccgga gcgcgcacca cgatcgaacc gtcccgggag gtgatcgccc cggccggagc
    28201 cgccagccgt gtgcgacgcg gcccgtaagg ctcccccccg accgctgcaa acatggacag
    28261 cacccaacct gccgccgtgg ccaaccgcct gatccgactg tcgtgtccca tacccgcttc
    28321 cgcccttttc cgtccgcgtt ccgaccgcgc acgcttgatt cgtcaccggt attatgcaac
    28381 actggagcgg ctgttttcaa tggaaggttt gccccatgct atactttgaa tgcggctcga
    28441 aatccggcgc gctcgaccgc acgcgactga aacaaggact cgttgaggcc cttgaccgcc
    28501 tgggtccgcg ccaaaaggtg ttggtgctgc cgccggactt cacccggttc cactcgcagg
    28561 ccggcctgct gacccagtac gtgtgggagt attacggcga ccggctggcc gccgtgatgc
    28621 cggccttggg gacccattcg ccgatgaccg aagcgcagat cc
//
LOCUS       DAOSLE010000018        25246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2195_length_25246_cov_7.820174, whole genome shotgun sequence.
ACCESSION   DAOSLE010000018 DAOSLE010000000
VERSION     DAOSLE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 25246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25246
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2195_length_25246_cov_7.820174"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..845)
                     /gene="nudC"
                     /locus_tag="PK576_02600"
     CDS             complement(108..845)
                     /gene="nudC"
                     /locus_tag="PK576_02600"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOE60509.1"
                     /translation="MKQCLCFRDERLWIRRSPAGTEWLFETNPNAQILPVEPLPDGTW
                     AAESESAPVPGAESIGLRELFDLVSAETSQNAGMARQLLHWRRSHRFCSVCAAPLERH
                     PTERAMYCLACGHLAYPRINPVAITLIHRGREVLLARKAGNQYPFWSLIAGFVEVGET
                     LEQTVRREVHEEVGLRVRDIRYATSQAWADPSNLMIGFTASYDGGEILPDGREIAEAD
                     WFGCSCLPPIPSRISIARRLIDAFFTA"
     gene            complement(842..2800)
                     /locus_tag="PK576_02605"
     CDS             complement(842..2800)
                     /locus_tag="PK576_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60510.1"
                     /translation="MKRLGEPLFARDETGRLKTRIGTFFLRTPGLVTLPGVHATQRIA
                     WLNELNRERQVAGIPALSEAESDAEIACSVDLLFNDQHVLIRPDPYAVDLAFEADEAL
                     QALVSKRKIRFLNTQNEIIRNALRARGENWRMSRVPVSPEEMNRMIAQARVAISGLPL
                     YFHNAATGTRFLTLETFAWLGTQPDDVFRKHLAEIVRYSSLRNRFGYPEIDIFPVGCA
                     FSRQAFEALNAEGLEIGALRAAYRKLLAAFREAVPIEFHEETFENLDWRNRLCSTLIH
                     QPNAVATQDLPDGMAPEFHLQIEWLPGCRIDEGELIFDPVYEELDLMPEDAELRAFCD
                     PRAREIIFNYVREFTTIEFINVGRITRSLSRRVQTGEHRTDVYVVQIKETGKPKPELC
                     ILRFQKWGVCEHLDEGKDMVRSIMEAVDYTDYVLDRRLGCRQLGMNLPEKVSTGRMPT
                     LYTGSNSLYKGAKCWTVYFERQYIPGQVTDKILTRHYANPEFNLLLARLLGEAAVVNC
                     VVGRANTEGQVLFDDGDEVITLDQAGMPERLTVSDHTGAFTLYEEPLEQMAEAYADPV
                     NKRIALMPNPEAFAETYLASFRQRFVTLQQEYRRRRHAFDKLFKHRPVDPAGSLAYRW
                     QCILARLDATDAETLTEAIRSQIRGLHP"
     gene            complement(2797..3708)
                     /gene="folD"
                     /locus_tag="PK576_02610"
     CDS             complement(2797..3708)
                     /gene="folD"
                     /locus_tag="PK576_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOE60511.1"
                     /translation="MQATILDGKVLAAELRAEIARETAEFKRQSGLTPGLGVILIGAD
                     PASVSYVTAKEKACAEAGMVSQDVRVPAETTQAEVLALVNRMNEDSRIHGILVQLPLP
                     RHINESAVLNAISPDKDVDGFTPANVGRMILGEPCYLPCTPHGIIRMLLKAEIQTAGR
                     HAVVVGRSNIVGKPVAHLLMRKAEGGNATVTVCHTGTRDLAEHTRRADILVVAAGRPN
                     TVTGDMIRPGAVVIDVGVNRIPDPSRPSGFRLVGDVDFDSARRVASLITPVPGGVGPM
                     TITMLLHNTLEAARRAAAHTVPGGPNP"
     gene            complement(3966..5546)
                     /locus_tag="PK576_02615"
     CDS             complement(3966..5546)
                     /locus_tag="PK576_02615"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOE60512.1"
                     /translation="MAKRVRDAWGSRIGVVLAAAGSAVGLGNFLRFPGVAARNGGGAF
                     MIPYFIAFFFLALPLCWMEWAMGRYGGQFGHGSAPGVFDAVGGRRHPALKYFGVLGVT
                     GALGIFFYYIYIESWTLAYSVFALTGRYAAIETPDCMGAFLSSYLGGNGDFFQSRLSA
                     YGFFLVTFAANFRVVYHGISHGIERFCKIAMPLLFLTAVVLVVRVFTLGAPVNPEWTL
                     DRALGFLWNPDFTALRSGKVWLEAAGQVFFSTSVGIGVLLTYATYLKPHDDALLSSTT
                     ASFLNEFAEVVLGGSIVIPAAFLFFGPVGTTEAVEGGTFGLAFRTTPLIFNHMAGGAW
                     VGFAWFALLFIAGVTSSVSILQPAVAFLEEEFGFNRKRCVIVIGLLCFVVAHLAIFGD
                     GVVDEMDFWFSSFGLPMFGLIEVCVFLFFFGPSRGWDALHTGSVLRLPRFFKFVMTFI
                     TPVFLFAILAGWLVTDGWKTILMRKTVDGQLVPLYTPEQVPWVLATRLFYLALVVGTC
                     LLIRRAWAHRNWAEPSVR"
     gene            complement(5630..6985)
                     /locus_tag="PK576_02620"
     CDS             complement(5630..6985)
                     /locus_tag="PK576_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOE60513.1"
                     /translation="MAVCLVILGTTGATCAEAFPEMYPKYVFLFIGDGMGATHVRATE
                     CYLRDAQIQRGVAAGMMKGLIMLALPVQGRATTFAKNKATTDSAASATALSSGYKADN
                     GVLNIDLETERKFDVMAQFVKRQGMKMGVISSAAPNHATPAGFYAHAKNRNMYPEIAM
                     QLAVCGLDYVGGPPMLGVEDTDAKAKKAAANNGHTIVEDRKSFDALKPGVKCVWVRSD
                     MPLVIDGKQEIPLADHVSKAIELLAGPEGFFMMVEGAAIDWASHSNDGAAMVQELLEF
                     DRAIARAVEFYRARPDEVLIVVTADHECGGLSLIEGPLTAPGMSKILDGQKKSRNASA
                     EMFKKFEKEKITFDEALPQMKAFFGIQHLSDEELAKCREIFDKGGDEQGKHAYGDERR
                     ISLFWSHLVSARAGFIWGSLNHTDDPVPVYAVGVQAARFAGSSDNALIGRYLRDLISG
                     K"
     gene            7318..10521
                     /locus_tag="PK576_02625"
     CDS             7318..10521
                     /locus_tag="PK576_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE60514.1"
                     /translation="MNVIGWVVVVVWGLCLCGRGADAREWENEQVNAVNREPARAAGF
                     PLADARAALTPEEPQTPFRVSLNGMWRYQWSGSPDDRPAGFHEPEFDDARWFTLDVPS
                     CVEMRGFGIPIYTNVKYPHARTPPKIGTAYNPVSAYRTRFTVPETWAGRPVFVRFDGV
                     YSAFYLWVNGQYVGYSEDSKLPAEFNLTRYLKPGGNVMAVEVYRWSDGSYLEDQDMFR
                     FSGIYRDVSLFSPPTAELRDFFVIAQPDADCRTAVLRVTSRARALGGRAVAATVRGEL
                     YDAENKPVEAALPETRLTLPADGTDVQAHVESPVGKERPPRLWSAEDPYLYTLVLTLE
                     GDDGSRDIRSCKVGFRRVDIREGKLLFNGRAIKFKGVNRHETSPENGRTISREDMLRD
                     ILLFKRHNIDTVRTAHYPDHYYWYQLCDRYGIYVVAEANVESHGMGYGKESLSHVPSW
                     EKAHVERNVNHVENYKNHPSVVMWSLGNEAGPGKNFKAAYDAVKARDATRPVHYERAN
                     DIVDVDSHMYPTVEWLYERGKNTAKPFFLCEYAHAMGNALGNFKEYWEAFYSSESLSG
                     GCIWDWIDQAVWKETDRVGPDGRRLRYLAYGGDFDDQPNDGNFVCNGVIGPTREITPK
                     LIEVKRVHQNLVVTAENAAAGRVEVWNRFGFTNARVFDMRWSLACDGREIAAGRVDAP
                     DVPPLSRRTVSVPVPKVTPRSGAEYFYRVSFHLREKTAWAETGHEIAASQLAFTPPQA
                     APSPSPAARGPVPGVRVRESRRAVTVSGRRFEAVFSRASGTLASLVYDGRTVICDRAG
                     ITHGPRLNTFRAFVDNDNQFGKWLRAEYMDSGLSQMSHHARPLQVERVDDATVLVRAT
                     VEACGFKSGRFTHEAEYRITGDGAITVRNRITPAGSLPPLPRMGVRMMLDGALERMAY
                     YGRGPWENYVDRNTGSDIGYYTGTVTEQYVPYVRPQECGGKSDVRWAAFLDRNGRGIH
                     FTFPQPLFVTALHYTSEDLEGARHRKGMERLFNPPTPRSEVCLALDIAQMGLGGASCG
                     PIPMEKYILRARPFAYTYIMRPCVKGSHAELSDLARP"
     gene            10595..11326
                     /locus_tag="PK576_02630"
     CDS             10595..11326
                     /locus_tag="PK576_02630"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOE60515.1"
                     /translation="MVRLGVNIDHVATLRQARGTTYPSLTEAAALCEQAGAHGITIHL
                     REDRRHIQDADVYALRAALKTRLNLEMANNPDVLAVALDVRPDEVCIVPERRQELTTE
                     GGLDAAGLQGQLAPTVAALRAAGILVSLFIEPDERQVEAAAALGAPFIELHTGTFAAR
                     TGEAREAELARLAHAAVRAHEFGLRVNAGHGLTVENVGAILEIPHLDTLNIGHSIVCR
                     AIAVGLAQATREMLERIGGGNREDA"
     gene            11381..12382
                     /locus_tag="PK576_02635"
     CDS             11381..12382
                     /locus_tag="PK576_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOE60516.1"
                     /translation="MSLQRGTYGIEYDERSGTSFRMSRLIVALLPVVAVVVLFARGCR
                     NEGETESDDGKTVGPAHYRLAESETPHERPSLWSSLFQREKPSDAGTAPEPVSGWRTA
                     GVTPPPETLVQSPEIRRLLEQAGACVEEDDLVGARRFLREILLRRDADSVRAFAEREI
                     GRLNIKLLFADRPMPEKVKHRVASGEVVSRLAKRYGNTPELILKANGIDRPERLRIGR
                     EIWVLDAPVFDLTVYKNGGSAVLTLNGSFFKRYPLLGGIPVKAPSGTYALRGKVALDG
                     VWRIMLAATGATPEAEGLALHGGADTPATDDRIRFATSDIEELSVLLPVGAAVNIAE"
     gene            complement(12451..13266)
                     /locus_tag="PK576_02640"
     CDS             complement(12451..13266)
                     /locus_tag="PK576_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE60517.1"
                     /translation="MTSHTAFQEALRTTGRYATVSERHQQNPIRIRALSSFLYAFTII
                     GIAVRCTVRHLFRALDYEEWGRICMASVRFAERLGGIVTFEGFEQRAVYDGPVVYVSN
                     HMSTLETMAFPTTLLSFGKIAIVLKKSLTEIPLVGKAALHIGCIPITRKNAREDLKTV
                     LEEGAKRLAEGHSVLLFPQGTRQAVFDGKKFNSLGSKLAERAGVPVVPLAVKTDFLQT
                     GKWFKDFGTVDPNKPIRFACGPVLPAMLGARATHDQSVAFIESKLKEWGLPTA"
     gene            13414..14715
                     /locus_tag="PK576_02645"
     CDS             13414..14715
                     /locus_tag="PK576_02645"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOE60518.1"
                     /translation="MIPQWVIEKKRDGGTLEEAEIRDFIEGFTRGSVPDYQMSALAMA
                     ILFRGMNAAETAVLTDAMMRSGDLVSFEGWPRPTADKHSTGGIGDKLSLMIAPLAASA
                     GLAVPMIAGRGLGITGGTLDKLESIPGYNTCLSVEAFKRVIAEVGCAIIGQTDRLAPA
                     DKKLYALRDVTGTVPSIPLITASIMSKKLAAGAETLVFDVKCGPAAFMRTREDARALA
                     EGLLAVGRALGRRTAALITDMAQPLGRTVGNAVEVAEALETLKGGGPPDTRELTIELT
                     AQMTLLAGVYADPTEARRELAARLDDGRALECFCRMVRAQGGDARVADDPRRLPQPGA
                     VREIPAPQDGIVATVDAEKLGRVVLRLGGGRTVTTDEIDPAAGIDRLVQTGERVEKGQ
                     PLMRLLAKNAACFDPLAAEAAAAVRITGEPVAERKLILENL"
     gene            14726..15187
                     /locus_tag="PK576_02650"
     CDS             14726..15187
                     /locus_tag="PK576_02650"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOE60519.1"
                     /translation="MSDPSDRNVAMKKEKLPEVPKSLRDSLVKAAREAAVKAYVPYSD
                     YPVGAALLTPDGQIYTGCNVENASYGLSVCAERVALFKAVSEGCRSFTALAVAGGQRR
                     AAVPCGACRQVLAEFCAPGMPVFSARLKGGTVTSATLAELLPLAFTGSDLG"
     gene            15469..19368
                     /locus_tag="PK576_02655"
     CDS             15469..19368
                     /locus_tag="PK576_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60520.1"
                     /translation="MRAAAMLCAIAVSLCADDTMITTGDYYGTLWGDGRLWTSGSAPT
                     GAGITLDTGSGSPQIRMDETNRMAGRITSTFGTMPAEGGQFLFSVVNNGFRHTDTLAP
                     QGIFGNNFHLTTNTFTFDNNGNDAQIRIEDGTGGNGGTARKPYRYLFDVPVVLADNLA
                     IRHLFTGTNQYGSYTTSQGDGPAAGVIFSRKISGGGKGITIENTAATLGVIFMGDSEF
                     TGDLTVNRGLARAKDTSNLPRVGTPFGQANSVIATNDEAGVDLGGFITGAGQTLRLKG
                     KRTCLGGTNGVLFSTWARPGDLSEWQGNVILEGETWIGGTLTGNNWPANFYPIDTDGG
                     LAISGPISEAGGSATLHVLNGRDTVFSGANTFSGGLYLDKGFFTARGAEAFGTGGIYF
                     NGGIYRVTSAEDADIFTADVHGAAGGFRVFLDAGVTCTMASNAVSGLVKPAFYKYGPG
                     TLVLTNRVSTGTSLCIMGGTVVLDTTREGNVKFGPNNNNWEMDHVYLYNDATFVVRGD
                     PATVRTGAWYGTPQAGLSGTFRAEDGQRFNLAFNADNNLLGCVNLETDNSGAYFTGAG
                     SAGSHTYNSDYAFLVYEGSSWVYTSGGRAVPLPDSAYQTTWGGKTDIVDVTPALAAVP
                     VPASTEADVIRFNTPNGGAEIVLTLAGDLTLDGRTILVTPAMGNTPVRITGGAIKSSG
                     LIRILNFNTNATLTIESDLAKNVANTQLITGGPGKTFLKGAKTFNGLVYVVGGELEVD
                     CADALGTANAAWNSGYVFLSNGGVLSFNGVINPVLSATTRTEFSLRVSSTGGGLKVPD
                     ADDVLTLSENGINVCGGGLFRKTGAGTLELKNSAKMSTQDEGGYKPNFARTDFAEGTV
                     IAATGFQEGAETMTVRNGVSLKGPRWIAPSQAGANGGGNRMELQAKRQLVAGAGGATV
                     DLQGQNVSLADSRSFYGLDKSTEFFGGTGEIRVVNTSATEAKIQCYGYHDAAFRGKFT
                     SHVPVTGEQGLGFRNAEWCVPEGVTNTFTQIAEDRFMLWFGRLSGKGTLTAQAIQTYI
                     PGRFFLGQDDVPAADFEGTLGVYCGGNSIGALQLVKVGNNAQRISGAANTFEGHTIIR
                     AGSLLVGNNSPATAGASGALGQAMVYVGDAETPAGASPALLADGAYTIANEIRVHDFS
                     PASATPVLGGTANADGALFSGAVRLYRDVIFRAETGTTVTFTGEIEGLKGAGMSGGGR
                     VVLADDVTLGGGFVWTGGTLQVNGAFTIPEGAALTVDASVCTKENRGTIFTLIRANGG
                     IFGTFMFAQELPAGWRVATRAGAVCLLFDAGTTLFLK"
     gene            19397..21280
                     /locus_tag="PK576_02660"
     CDS             19397..21280
                     /locus_tag="PK576_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60521.1"
                     /translation="MKRHTHRCWGSVLAAGFTAFAVQAVDITFPNTDGKGDLASTASW
                     NQTPIPDPTNRPEFKATLMGATLTASEDISFAGFKINVNNTAYPVILDMRNEATGANP
                     GPRKITLSDNIRNVSGNNITYIKGGEWTGTSISTDWGKSTDIYYVQGAVMTLSGGLAG
                     TYGGNGALIELSGGSVVTALHARAQYNYGDNSSIQVLDGSKLVVTGVADESFMSDKSS
                     GFNNGYVVRGENSLIKKTSSAKAALLGAGGHSAYLRVEDRGVADFTGTLRVGNAANST
                     NNVVSVTGGGTLKADGVQLGYSSSAGNNLFAITNGGVAEVPNGFFVGGNAYEGCFGNT
                     LLVSNGVFRGKVPTIGFKAGSSNNLVRIVGRQSVYEAVANVSSDTACIFAYERNNRFE
                     LDDAVWTNNVAKITYLNCSQFTEGHCVMELRNNAEFYTTNQFYMTGIHYRSLSNLLSI
                     VSGSRLYSKGSVGVYSIGNVFSISNGTLETEGSFTVTAQSGSPMMYGYSNNWVRVAGE
                     RPRISVTNGTFAVQYASYLQFDIPASGYEAGAAPVKAKAFTVSSDSSLIVNGLDARIK
                     AIKEPEVVALVESLNGVTVPAGVLATANAGLPKGCALFVSADNKALLLRVYKETGTLL
                     SIH"
     gene            21293..23698
                     /locus_tag="PK576_02665"
     CDS             21293..23698
                     /locus_tag="PK576_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60522.1"
                     /translation="MMSLFALPVLAAALSAVTSNPSNNFFVAGHEAKATLTAKGLAAG
                     EKRPVRLEVYDFTYTNRVATVPGEIVADEKGEWRGEFTLPSDRYGVYLVRPVAAGLEL
                     PKRGTVPKGAFTYAVLADPSEMPEIDLWDTFLGLHGGSYSWMYQRGGLGDARKPSEDR
                     FIVANLDLFNREKDFWKSFLTNEAVQAKYRADVTGYVKAAVAAGGGKQKRRFYEPTWE
                     ANLKTPNAETLVKVHQIAWEVIHELDPDAYVGAPTSAGINDLAFLRSLFDLGLAKYMN
                     AFAVHPYIAAPPEANGLVDTVRSLKRMLREYMGRDVPMIGTESGLNEPNSPLGEKTKL
                     TGQLRQNLILFGEGFLWNLPFYGYDFGADANNQDEGDYGVCYNPLYPKHRWGPGEVQP
                     RPVFGALAAFGRLTEGHRPTCPIEWLGETVLGYAFADKSDSHCVIALWDWGERGTEVE
                     LPVGRDAIDVADVMGNVTRKKTTGGTLKLTLSEYPQYVLDANSAIWGKAAQAKLKWGE
                     RKYMSDSDRAPVGIADLAPSFASGGPGVAVTLENRTDEPHRVALETRIPGEPDARVKR
                     SVSLAARQTQRIEIAFDGFTPDPTVFFDVEVRVVPEKGMVATAVDSFNFLPVPSSFAF
                     DGGKTVSLGYDARFLTFDIFIPDSTPTNGWTGWDSWKGDSVQVGLAKSALKKRTQNDL
                     ADNAAEAKSEITFALTPNGPEMYRTITWDVRRFPCDTHLAGQIDLKEAPLTVEKSAKG
                     FRYRASVPWAFLNLSAPKPGDTFRFAFQFNDREPGNRFLKESKGFELKQAAPKKFGWG
                     TIR"
     gene            23695..25008
                     /locus_tag="PK576_02670"
     CDS             23695..25008
                     /locus_tag="PK576_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60523.1"
                     /translation="MTALLLAASLQFHGVMGQSAAKGEAPLEFTGVSFLSEGPCGRVF
                     FGGDDGKLYELKDGEIIRTGVRMQGRFLDWDGKTVRALGQHDVWEVDPATLAFKHVIR
                     TRDFRFDLAAVVPDDPVHPFTAKGKYVTYDKKRDALLVLDEKGAERGGLFPLPERLSK
                     SEIISLGFLPDSGDLVAVSYYPDLRIYRFTAEGTQVTGHGWPARRGFGFFRKSGGELY
                     HCGTPTLVRMEENLAGAQGNVFRVGKEHFTCGYAKQGDREYIGTSQGLYVKEPGETEF
                     RKRLGGIRPLTALAVNGKYVYMSMGGTIRWMYLDEDENGPFASSDDHIFRVNGGWTEK
                     PYDLIPDGDNLLVAAGKLGTWRFKPFLPPAYAFNRKHWEQVSTNVCTELSVGKPSCEL
                     LGFLSSAEVPDGLEPGKIASQGKWLVVEDRKNFRLVRFRIDADKK"
BASE COUNT         4865 a         7419 c         8305 g         4657 t
ORIGIN      
        1 ccgcctttga ggttgcgggt ttcgcccgca tctcaatacg tcagccccgc cgcacttgcc
       61 cggacgggca gacgctcgtt gctcccggcc gcaggctgcg ccctgtttca tgccgtgaaa
      121 aaagcatcga tcaggcggcg ggcgatgctg atgcgtgacg ggatcggcgg cagacacgag
      181 catccgaacc agtcggcctc ggcgatctcc ctgccgtcgg gaaggatctc gccgccatcg
      241 tacgacgcgg tgaagccgat catcaggttg ctggggtcgg cccaggcttg gctggtggca
      301 tagcggatgt cgcgcacacg cagacccacc tcttcgtgca cttcgcgtcg gacggtttgt
      361 tccagagtct ccccgacctc cacgaaaccg gcgatcaggc tccagaaagg gtactggttc
      421 ccggctttcc gcgcgagcag cacctcgcgc ccccgatgaa tcagggtgat ggcaaccggg
      481 ttgatccggg gataagcgag atgtccgcag gccaggcaat acatggcgcg ttcagtgggg
      541 tggcgctcca gcggcgcggc gcacacgctg cagaaacggt gtgaccgccg ccagtgcagg
      601 agttgccgag ccatgcctgc gttctgcgag gtttcggctg acaccagatc gaacagttcg
      661 cgcaacccga tggactccgc gccggggaca ggtgcagact ccgactcagc ggcccaggtg
      721 ccgtccggta gcggttcgac gggcaggatc tgcgcgttcg ggttggtctc gaacagccat
      781 tcggttcctg cgggcgaacg ccggatccac aaccgctcat cccggaaaca caggcactgt
      841 ttcatgggtg cagtccccgg atctgactgc gaatggcctc tgtcagcgtt tcggcgtctg
      901 tggcgtcgag ccgcgccaga atacattgcc agcggtaggc gagactgcct gccggatcaa
      961 ccggccggtg tttgaacagt ttgtcgaatg cgtgacgccg ccgccggtac tcctgctgca
     1021 acgtcacgaa ccgctgccgg aacgacgcga ggtaggtttc ggcaaaagcc tccggattgg
     1081 gcatgagcgc gattcgcttg ttgaccggat ccgcataggc ctccgccatt tgctccagcg
     1141 gctcttcgta aagggtgaag gcaccggtat ggtccgagac ggtcaagcgt tccggcatac
     1201 ccgcctgatc cagcgtgatc acttcgtcgc cgtcatcgaa gaggacctgt ccctccgtat
     1261 tggcccggcc gaccacgcag ttgaccacgg cggcctcgcc caacagccgg gccagaagca
     1321 ggttgaattc cgggttcgcg tagtgccgcg tcaggatctt gtcggtcact tgtccgggga
     1381 tgtactggcg ctcgaaatac acggtccagc atttggctcc tttgtacaag gagttcgagc
     1441 cggtgtacag ggtcggcatc cggccggtcg agactttctc gggcagattc atgccaagct
     1501 ggcggcatcc gaggcggcgg tcgagcacgt agtcggtgta atccaccgcc tccatgatcg
     1561 agcggaccat gtctttgccc tcatccagat gttcgcagac cccccatttc tgaaaacgaa
     1621 ggatgcaaag ttccggcttg ggttttccgg tttccttgat ctgcacaacg tagacgtcgg
     1681 tacggtgttc gccggtctgc acgcgacggg acagggagcg cgtgatgcgc ccgacgttga
     1741 tgaattcgat ggtggtgaac tcccgcacgt aattgaagat gatctctctg gcccgcggat
     1801 cgcaaaaggc gcgcagttcg gcatcctccg gcatcaggtc caactcttcg tagaccggat
     1861 caaagatcag ttcgccctca tcaatccggc agcccggtag ccactcgatc tgcaaatgga
     1921 attcgggggc catgccgtcc gggaggtctt gggtggccac cgcgttcggc tgatggatga
     1981 gcgtggagca gagccggttg cgccaatcaa ggttctcgaa cgtttcctcg tggaattcga
     2041 tcgggacggc ttcgcggaag gccgcgagca gcttccggta ggcggcgcgc agcgctccga
     2101 tttcaagccc ctcggcgttc agggcctcga acgcctgacg ggagaaggca cagcctacgg
     2161 ggaagatatc gatttcaggg tacccgaacc ggttacgcag cgagctgtag cgcacgattt
     2221 cggcaaggtg cttgcggaac acgtcgtccg gctgcgtccc cagccaggca aacgtttcca
     2281 gcgtcaaaaa gcgtgtgccc gtggcggcgt tgtggaaata gagcggcaga ccggaaatgg
     2341 cgacccgcgc ttgggcgatc atccggttca tctcttccgg cgaaaccgga acccggctca
     2401 tacgccagtt ttcgccgcgc gcgcgcaacg cattgcggat gatctcgttc tgcgtgttga
     2461 gaaaacggat cttacgcttg gaaaccagcg cctgaagcgc ctcgtccgct tcaaacgcca
     2521 ggtcaacggc gtagggatcg ggccggatga gcacgtgctg gtcattgaac agcaaatcca
     2581 ccgaacacgc gatctccgcg tcgctttccg cttccgagag ggcggggatc ccggcgacct
     2641 gtcgttcacg gttgagttcg ttgagccagg cgatacgctg cgtggcatgc acgccgggca
     2701 gcgtgaccag tccgggggta cgcagaaaga acgtgccgat ccgggttttc agacggcccg
     2761 tctcatcccg cgcgaagagc ggttcgccaa gacgtttcat gggttaggtc caccgggtac
     2821 ggtgtgcgca gccgcgcgcc tcgcggcctc cagagtgtta tgcagcagca tggtgatggt
     2881 catcggcccc acgcctccgg gaaccggggt gatcagcgag gcgacgcggc gggccgaatc
     2941 gaagtccaca tcgccgacaa ggcggaaccc gctcggacgg gaggggtcgg ggatacggtt
     3001 cacgccgaca tcaatcacga cggcaccggg ccggatcatg tcgccggtca cggtgttggg
     3061 ccgacctgcg gccaccacca gaatgtcggc ccggcgcgtg tgctccgcga ggtcgcgcgt
     3121 gccggtgtgg cagaccgtca cggtggcgtt gccgccctcg gctttgcgca tcagcagatg
     3181 cgcgaccggt ttacccacga tgttgctgcg gcccaccacg acggcatgcc ggcctgcggt
     3241 ttggatctcg gctttcagca gcatccggat gatgccgtgc ggggtgcagg gcagataaca
     3301 gggttcgcca aggatcatgc gtccgacgtt cgctggcgtg aatccgtcca catccttgtc
     3361 cggggagatc gcgttgagga cggctgattc gttgatatga cgggggagcg gaagctggac
     3421 caagatgccg tgaatgcggg aatcctcgtt catgcggttt acgagggcca gcacctctgc
     3481 ctgcgtggtc tcggctggca cacgcacatc ctgcgaaacc atgcccgcct ccgcacatgc
     3541 cttttctttg gcggtgacat aggagacgga tgcaggatcc gctccgatca gaatcacgcc
     3601 gagtcccggc gtcaggccgc tttgccgttt aaattccgcc gtctcgcggg cgatctccgc
     3661 ccgcagttcc gcggccaaca cttttccgtc caggattgtc gcttgcatcg tgctcccgtg
     3721 agggggggtt atccgaaaag cccgtctata ataatcgaac aaggcccttt tgtctatgcc
     3781 ggcgccggtg cgccgggcgc atccgcgatt ccctgacttc ggaaatcttg gcttgtctta
     3841 acgttgacct gcgacccgcg acattcgccc tgcggcgggt agggacggtt ccccgaaccg
     3901 tccgcagcgt gaaccctgaa acccgaaacc aaagggcgtt caaagttcaa cggctctttt
     3961 ggggttcaac ggacggatgg ctctgcccaa ttccggtgcg cccacgcgcg gcggatgagc
     4021 aggcaggtgc cgacgacgag ggcgaggtag aagagacgcg tggcgagcac ccaggggacc
     4081 tgttcgggcg tgtagagcgg cacgagttgg ccgtcaaccg tcttccgcat caagatcgtt
     4141 ttccagccgt cggtcaccag ccaccccgcg aggatcgcga acaggaagac cggggtgatg
     4201 aaggtcatga cgaatttgaa gaagcgcggc aaccgcagta cggaaccggt gtgcagcgcg
     4261 tcccagccgc gcgacggccc gaagaaaaac agaaagacgc agacttcgat caaaccgaac
     4321 atcggcaggc cgaacgagct gaaccaaaag tccatctcat ccacgacacc gtcgccaaaa
     4381 atcgcgagat gtgccacaac gaaacaaaga agcccgatga cgatcacgca gcgcttgcgg
     4441 ttgaagccga actcctcctc caggaaggcc acggcaggtt gcagaatcga gaccgagctg
     4501 gtgacaccag cgatgaaaag cagtgcgaac caggcgaacc caacccaagc gccgcccgcc
     4561 atgtggttga aaatcagcgg cgtggtgcgg aaggcaaggc cgaaggttcc gccttcgacc
     4621 gcctcggtgg tgccgaccgg cccaaagaaa agaaaggcgg cggggatgac gatcgagccc
     4681 ccgagcacga cttcggcgaa ttcgttcagg aacgaggcgg tggtggagga gagcagcgca
     4741 tcgtcgtgcg gtttcaaata tgtggcatag gtcagcaata caccgatgcc cactgaggtt
     4801 gagaagaaga cctggcctgc ggcttccagc cagaccttcc cgctacggag cgcggtgaaa
     4861 tcggggttcc acaggaaccc cagagcgcgg tcgagtgtcc actctggatt gaccggcgca
     4921 ccgagcgtga aaacgcgcac caccagcacg acggccgtga ggaagagcaa cggcatggcg
     4981 attttacaga agcgctcgat gccgtggctg ataccatgat agaccacccg gaagttggcg
     5041 gcgaaagtca ccagaaaaaa gccgtaggcg gaaagccgac tctggaaaaa gtcgccgtta
     5101 ccacccagat aactgctgag aaaagcaccc atgcagtccg gcgtttcaat cgcggcatag
     5161 cggccggtga gcgcgaacac gctgtaggcc agtgtccacg attcgatata gatgtaatag
     5221 aaaaagatgc ccagcgcgcc ggtgacaccg agcacgccga agtatttcag ggccggatgg
     5281 cggcgaccgc ccaccgcatc gaagactccc ggtgcgctgc cgtggccgaa ctgtccgccg
     5341 taacggccca tggcccactc catccagcag agcggcaggg cgaggaaaaa aaaggcgatg
     5401 aagtacggga tcatgaaagc cccgccaccg ttacgcgcgg ccacgccggg gaaacgcagg
     5461 aagttgccga gaccgactgc actgccggcc gccgctagga cgacgccaat ccggctgccc
     5521 caggcgtcgc gaactctctt agccatggat cttccctctt acacgcgaat atttatcaag
     5581 cgtgaacagg gtaccgtatc gcgaccggat aggatagctt tttcgttctt tactttccgc
     5641 tgataagatc gcggaggtag cggccgatca gcgcgttgtc actgctgccg gcaaagcgcg
     5701 ccgcctgcac accgaccgca taaaccggga cgggatcgtc ggtatggttc aagctccccc
     5761 aaatgaaccc cgcccgggca ctgacgagat gcgaccagaa caggctgatt ctgcgctcat
     5821 cgccatacgc atgtttgccc tgctcatcgc cgcccttgtc gaagatttca cggcacttcg
     5881 ccagctcctc gtcgctcagg tgttgtatgc cgaaaaacgc cttcatctgc ggcaacgcct
     5941 cgtcaaacgt gatcttttcc ttctcgaact tcttaaacat ttcggcggag gcgttccgcg
     6001 actttttctg gccgtccaga atcttgctca tgccgggagc cgtgagcggc ccctcgatca
     6061 gcgacagccc gccgcattca tgatccgctg tcacaacaat caagacttcg tcgggccgcg
     6121 cccggtaaaa ctcgactgcc ctggctatgg cacggtcgaa ctccagcagc tcctgcacca
     6181 tggcggcacc gtcattggaa tggctggccc aatcgatggc cgcgccttcg accatcatga
     6241 aaaaaccttc cggccccgcc agcaactcga tcgccttgct cacatgatcc gcaagcggga
     6301 tctcctgctt gccgtcgatg accagcggca tgtcgctcct gacccaaacg catttcacac
     6361 cgggtttcag ggcgtcgaag ctcttgcggt cttcgacaat cgtgtgcccg ttgtttgcgg
     6421 cggccttctt ggccttggca tccgtatcct cgacaccgag catgggcggg ccgcccacat
     6481 aatcaagacc gcacaccgcc aactgcatgg cgatctccgg gtacatgttt cgatttttcg
     6541 catgggcgta aaaaccggcc ggcgtggcat ggttcggcgc ggcacttgaa atgacgccca
     6601 tcttcatgcc ctgacgcttc acaaattgcg ccatgacatc aaacttgcgc tcggtttcaa
     6661 gatcgatgtt cagtacgccg ttatcggcct tgtagcctga agacaaggcc gtggccgaag
     6721 ccgccgagtc ggtcgtcgct ttgtttttgg caaaggtcgt cgccctgccc tgcaccggca
     6781 aagccagcat gatcagcccc ttcatcattc cggcggcaac ccctctttgg atctgggcgt
     6841 ctctaagata gcactccgtg gcccgcacat gtgtcgcgcc catgccgtcc ccgataaaaa
     6901 ggaaaacgta cttggggtac atctcgggaa acgcctcggc acacgttgcg cccgttgttc
     6961 ccaagataac caggcaaaca gccatcagaa ctttaatttt ttgtgtaaac agaacactcc
     7021 tcatctcaac cctcctcttt tacaacacgc tcccgatatt cgcgtccaac gtgatagaag
     7081 gtaatgaacc ctattcttgt taggctaacg cgttgtttcg gtaagaaacg tgttagaaag
     7141 ggtcccgaac atcccatagg cggtacggct agcccttacc catgcgggtt cgctcgggtt
     7201 tccggcgtct tttttgaggt ttcagggaaa ccagggaaat cgcattgcgt cttggccgca
     7261 taaacggaaa tcgggtataa tgacggcatt aggaaaacct tttcaggaga atacggtatg
     7321 aacgtgatcg ggtgggtcgt ggttgtcgtc tgggggctgt gcctgtgcgg gaggggtgcg
     7381 gacgcgcgcg agtgggagaa tgagcaggtc aacgccgtga accgtgaacc ggcgcgggcc
     7441 gccggctttc cgctcgccga cgcgcgcgcc gcactgacgc cggaggagcc gcagaccccg
     7501 ttccgcgtgt cgctcaacgg catgtggcgt tatcagtgga gcgggagccc tgatgaccgc
     7561 ccggccgggt tccacgaacc ggagtttgat gatgcgcgct ggttcacgct ggatgttccg
     7621 agttgtgtgg agatgcgggg gttcggcatc ccgatctata cgaatgtcaa gtacccgcat
     7681 gcccgtaccc cacccaagat cggcacggcc tacaatccgg tcagcgccta ccgcacccgg
     7741 ttcaccgtgc cggagacatg ggcgggacga cccgtgttcg tgcgcttcga cggggtctat
     7801 tccgccttct acctctgggt gaacggtcag tatgtcggat acagcgaaga cagcaaactg
     7861 cctgcggaat tcaacctcac acggtatctc aagcccggcg ggaatgtgat ggcggtggag
     7921 gtgtaccgct ggtcggacgg ctcttatctc gaggatcagg acatgttccg cttcagcggc
     7981 atctaccgcg atgtctcgct cttctcgccg ccgactgcgg agttgcgcga ctttttcgtg
     8041 atcgcccagc ctgacgccga ctgccgcacg gcggtactgc gcgtcacatc ccgggcgcgc
     8101 gctttgggcg gccgggcggt tgccgcgacg gtgcggggcg aattgtacga cgctgaaaac
     8161 aaacccgtcg aggccgcgtt gcccgagacg cgtctgacgc tgcctgccga cggaaccgac
     8221 gtgcaggcgc atgtcgagtc gcccgtaggc aaggaacggc ccccccgttt gtggtcggcg
     8281 gaagatccgt acctctatac actggttctc acgctcgaag gtgatgacgg ctcgcgggat
     8341 atccgctcct gcaaggtcgg attccgccgg gttgatatcc gcgagggcaa gctcctcttc
     8401 aacggccggg cgatcaagtt caagggggtg aaccgccacg agacctcgcc ggagaacggc
     8461 cgcacgatca gccgggagga tatgctgcgg gatattctgc tcttcaagcg ccacaacatc
     8521 gacacggtcc gcacggcgca ctatccggac cattattact ggtatcagct ctgcgaccgg
     8581 tacggtatct atgttgtggc cgaggcgaac gtcgagtcgc acggtatggg gtatggcaag
     8641 gagtcgctca gtcatgtgcc ctcatgggag aaggcgcatg ttgaacgtaa tgtgaaccac
     8701 gtcgagaact acaagaacca tccttcggtt gtcatgtggt cgttgggcaa cgaggccggt
     8761 cccggcaaga acttcaaagc cgcgtacgac gctgtcaagg cgcgggatgc cacgcggccc
     8821 gtgcactacg agcgggccaa tgacatcgtg gatgtcgaca gccacatgta cccgacggtc
     8881 gaatggttgt acgaacgcgg caaaaacacc gccaagccct tcttcctctg cgaatacgcg
     8941 catgcgatgg gcaacgcgct cggcaacttt aaggaatact gggaggcctt ctactccagc
     9001 gaatcgctct ccggcgggtg catctgggac tggatcgacc aggcggtgtg gaaagagacc
     9061 gaccgggtcg ggccggatgg ccggcggctg cgctatctgg cttacggcgg cgatttcgat
     9121 gatcagccga acgacggcaa tttcgtctgc aacggcgtga tcgggccgac ccgtgagatc
     9181 acgccgaagc tgatcgaggt caagcgtgtg catcagaatc tggtggtcac ggccgagaat
     9241 gcggccgcgg gccgggtcga ggtctggaac cgcttcggct tcaccaacgc acgcgtgttc
     9301 gacatgcgct ggtctctggc atgcgacggc cgcgagatcg ccgcaggccg ggttgacgcg
     9361 cccgatgtgc cgccgctctc gcgccgcacc gtgtccgtgc cggttcccaa ggtgacgccc
     9421 cggtccggtg ccgagtattt ctaccgcgtg agtttccatc tccgcgagaa gacggcgtgg
     9481 gcggaaacgg gtcacgagat cgcggccagt cagttggcgt tcaccccgcc gcaagccgcg
     9541 ccgtccccgt ctccggccgc gcgcggcccc gtgcccggcg tccgggtccg tgaaagccgc
     9601 agggcggtga ccgtgagcgg tcggcgcttc gaggcggtct tcagccgggc atccgggacg
     9661 ctcgcctctc tggtgtacga cggccggacg gtgatctgcg accgtgcggg gatcacgcac
     9721 ggtccgcgtc tgaacacctt ccgcgccttt gtggacaacg ataaccaatt cggcaaatgg
     9781 ttgcgcgccg agtacatgga ttccggcctg tcgcagatga gccatcacgc gcggccgctg
     9841 caggttgagc gggtggacga tgccacggtg cttgtccggg ccacggttga ggcatgcggc
     9901 ttcaagagcg gccggttcac gcacgaggcc gagtatcgca tcaccgggga cggcgcgatt
     9961 acggtgcgga accgcatcac gcccgcgggc agcctgcccc cgctgccgcg catgggcgtg
    10021 cgcatgatgc tggacggtgc gctggagcgc atggcttact atgggcgcgg cccttgggag
    10081 aactacgtgg atcgtaatac ggggagcgac atcgggtact acaccggcac ggtgacagaa
    10141 cagtacgtgc cctatgtgcg gccccaggag tgcggcggca agagtgacgt ccggtgggcc
    10201 gcgtttctgg accgcaacgg ccgcgggatt catttcacgt ttccgcaacc gctttttgtc
    10261 acggctctgc actacacctc agaggacttg gagggggcgc gccaccgcaa agggatggag
    10321 cgcctcttca atccgccaac gccgcgttcc gaggtctgcc tcgcgctgga cattgcgcag
    10381 atgggtctcg gcggcgcgag ctgcggcccg atccccatgg agaaatacat tctgcgtgcc
    10441 cggccctttg cgtacaccta catcatgcgg ccctgcgtta agggcagcca tgcggaactc
    10501 agcgatttgg ccaggccctg atgcggcgcg gcgcggaaat cggggacagg atcggaaaac
    10561 ggtgttatgc tgtgaggtaa agaaggagga ccatatggtt cggctgggag tcaacattga
    10621 tcatgtggcc acgttgcgtc aggcgcgcgg gacaacgtat ccgagtttga cggaggcggc
    10681 ggcgctctgt gaacaggcgg gtgcgcacgg catcacgatc catctgcgcg aggaccgccg
    10741 ccacattcag gatgccgacg tgtacgcgtt acgcgcggca ctgaagacgc gcctgaacct
    10801 cgagatggcg aacaaccccg atgtgctggc ggtagcgctc gatgtgcggc cggatgaggt
    10861 gtgcatcgtg ccggagcgcc ggcaggaact gacgaccgag ggcgggctgg acgcggcggg
    10921 cctgcaaggg caactcgcgc cgaccgtggc cgcgttgcgc gcggcgggga ttctggtgag
    10981 tctgttcatc gagcccgacg aacggcaggt ggaggctgcg gcggcgctcg gtgcgccgtt
    11041 cattgagttg cataccggga cgtttgccgc gcgcacgggc gaggcacgcg aagcggagtt
    11101 ggcgcggctg gcgcatgccg cggtccgcgc acacgagttc ggactgcgtg tcaacgcggg
    11161 ccacgggttg acggtcgaga acgtcggggc gattctggag ataccgcatc tcgatacgtt
    11221 gaacatcggg catagcatcg tgtgccgggc gatcgccgtg ggtcttgccc aggccacacg
    11281 cgagatgctg gaacggatcg gaggcggcaa tcgggaggat gcgtgacggg tccggagggc
    11341 acggacccgg cagcattccc cctccggcgg agagggtcac atgtctttgc agcgcggcac
    11401 atacggcatc gaatacgacg agcggagcgg aacatccttc cggatgtcgc ggctgatcgt
    11461 cgcgctgctg ccggtggttg cggtcgtggt gctgttcgcg cgcggctgcc ggaacgaggg
    11521 ggaaacggag tcggacgacg ggaaaacggt cggtccggcc cattaccggc tggctgagtc
    11581 cgaaacgccg cacgagcgtc cttcgctctg gtcttcgctg ttccagcggg agaaaccgtc
    11641 cgatgccggg acggccccgg aaccggtctc cggctggcgc acggcaggcg tgacgccgcc
    11701 gccggaaacc cttgtgcagt cgcccgagat ccgtcggttg ctggagcagg ccggcgcgtg
    11761 cgtggaggag gatgacctcg tgggcgcgcg gcggtttctg cgcgagatcc tgttgcgccg
    11821 cgatgccgac agtgtgcgcg cgtttgccga gcgtgagatc gggcggctga acatcaagct
    11881 gctcttcgcc gaccggccga tgccggagaa ggtcaagcac cgcgtcgcat ccggagaggt
    11941 ggtcagtcgg ctcgccaaac gatacgggaa cacccccgag ctgattttga aggccaacgg
    12001 gatcgatcgt cccgagcgtc tgcgcattgg ccgcgagatc tgggtcctcg acgcgccagt
    12061 gttcgatctg acggtgtaca agaacggcgg gagtgccgtg ctgacgctga acggatcctt
    12121 cttcaagcgc tatccgctac tgggcggtat cccggtcaag gccccttccg gcacctatgc
    12181 cctccgcggc aaagtcgcgc ttgatggcgt gtggcggatc atgttggcgg cgaccggcgc
    12241 gacacccgag gcagagggac tcgccctgca cggcggggcg gatacacccg ccacggacga
    12301 ccgtatccgg ttcgccacct cagatatcga ggaactcagt gtgctgctgc ccgtcggcgc
    12361 tgcggtgaac atcgcggagt aggcgaatcg gggcgacctg actcgagcgg cctcgccctg
    12421 attcgacccg ggtcgattcg gggtgcggtg tcaggcggtc gggaggcccc actccttgag
    12481 cttggattca atgaacgcga cgctctgatc atgcgtggcg cgggcaccga gcatggcggg
    12541 gaggaccggg ccacaggcga agcggatcgg cttgttgggg tcgaccgtgc cgaagtcctt
    12601 gaaccacttg ccggtttgga ggaaatcggt cttcaccgcc agcggcacga cgggcacacc
    12661 cgcgcgttcg gccagctttg aacccagcga gttgaacttc ttgccgtcga aaaccgcctg
    12721 gcgcgtgcct tgcgggaaga gcagcaccga atggccttcg gccaggcgtt tcgcgccctc
    12781 ctccagtacg gttttcaggt cttcgcgcgc gtttttgcgc gttatcggaa tgcagccgat
    12841 gtgcagagcg gccttgccga ccagcggaat ctcggtcagg ctctttttaa gcacgatcgc
    12901 gattttgccg aacgagagca gcgtggtcgg gaaggccatg gtttccagcg tgctcatgtg
    12961 gttggacacg tacacgaccg gcccgtcata tacggcgcgc tgttcgaagc cctcgaaggt
    13021 aacaatcccg ccgagccgtt cggcgaagcg taccgaagcc atgcagatcc gcccccactc
    13081 ctcgtagtcc aaggcacgga agaggtgtct caccgtgcaa cggaccgcaa tgccgataat
    13141 ggtgaaggcg tagaggaaag aagagagcgc acggatccgg atcgggttct gctgatgccg
    13201 ctcggagacg gtggcgtaac gtccggtagt gcgcagggct tcctgaaacg cggtatgtga
    13261 ggtcatggtg cgggacacta taccaaaaac ttcggggcgc ggggacggaa aaaccgcagt
    13321 tgtgcgggcc cggcttttgg ggtatacttt cccccgaata atggggattg aaagagcggt
    13381 ttggaaagac cggtggacgg tgaaggaggg gacatgattc ctcaatgggt gattgagaaa
    13441 aaacgggatg gcggaacgct tgaagaggcg gagatacgcg atttcataga ggggttcacg
    13501 cgcgggtcgg tgccggacta ccagatgtcg gctctggcta tggccatcct gttccggggg
    13561 atgaacgctg cggaaacggc ggtgttgacc gacgcgatga tgcgttcggg cgacctcgtg
    13621 tcctttgagg gatggccccg tcccacggcg gacaagcact cgaccggcgg tatcggagac
    13681 aagctctccc tcatgatcgc gccgctggcg gcttcggcgg ggctggcggt tccgatgatc
    13741 gccgggcgcg gcctggggat caccggcggc acactggaca agttggagag cattccggga
    13801 tataacacat gcctttcggt ggaggccttc aagcgggtga tcgcggaggt cgggtgcgcg
    13861 atcatcgggc agaccgaccg tttggccccc gccgacaaaa agctctacgc gctgcgcgac
    13921 gtcaccggca cggtgcccag catcccgctg atcaccgcca gcatcatgtc caaaaaattg
    13981 gccgcgggtg ccgagacgct ggtcttcgac gtcaaatgcg gtcccgcggc ctttatgcgc
    14041 acacgggagg acgcgcgtgc gctggccgaa ggcctgctcg ccgtgggccg ggcgttgggt
    14101 cggcggaccg cagcgctcat tacggatatg gcgcagccgc tcgggcgcac cgtgggtaac
    14161 gctgtggagg tggccgaggc gctggagacg ttgaagggcg gcgggccgcc ggatacgcgc
    14221 gagctgacga tcgaactcac cgcgcagatg actctgctgg ccggggtgta cgcggacccg
    14281 acggaggcgc ggcgcgaact tgcggcgcgg ctcgacgacg gccgtgccct ggagtgtttc
    14341 tgccgcatgg tgcgcgcgca gggcggcgac gcgcgtgtgg cggacgatcc gcgtcgcctg
    14401 ccgcagcccg gcgcggtacg ggagattccc gcgccgcagg acgggatcgt ggccaccgtg
    14461 gatgccgaaa agctgggccg ggtggtgttg cggctcggcg gcggacgcac ggtcacgacg
    14521 gacgagatcg atcccgcagc cgggatcgac cgtttggtcc agaccggcga acgggtggaa
    14581 aaaggccagc cgctcatgcg gttacttgca aaaaatgccg catgctttga tccgttggcg
    14641 gcagaggccg cggctgccgt gcgcatcacg ggcgaaccgg tggcggagag gaagctgatt
    14701 ttagaaaatc tctgacgggt ctgacttgtc cgacccgtcc gacaggaacg ttgcgatgaa
    14761 aaaagaaaaa ctccctgagg tccccaagtc attgcgcgac agcttggtga aggccgcgcg
    14821 cgaggccgcg gtcaaggcct acgtgcccta ttccgattat ccggtgggag cggcgcttct
    14881 cacgccggac ggccagatct atacgggctg caacgtcgag aacgcctcgt atggcctgag
    14941 cgtgtgcgcg gagcgggtcg cgctgttcaa ggcggtcagc gagggatgcc gcagtttcac
    15001 ggccttggcg gtggcgggcg gacagcggcg cgcggcggtt ccctgcggtg catgccgtca
    15061 ggtgctggca gagttttgtg cgcccgggat gccggttttc agtgcgcggc tcaagggcgg
    15121 cacggtaacg tcggccacgc tggcggaact gttgccgctg gccttcacgg ggtcggatct
    15181 gggctaatag aataacgcaa accgatttga tcccgtgcga aagcggtgcc gtctgtcggt
    15241 tgttcccgca ctccacattt catgcggtat tgcgccctgc gcgtagtccg gtgcgttttc
    15301 gatattgacc ccttctgtcc cccccctgca taataaagag gtaactgtgg aaaatgaaaa
    15361 ggtaccgggg atgctcgtcg tgttgtttcg tgatcaggag acggtgaaat gaaaacaaaa
    15421 gcagtaaagc gtagttcggc tgggaatgtc gtgtctgtga gatcggtgat gcgagcggcg
    15481 gcgatgctct gcgcaattgc ggtcagcctg tgcgcggacg acacgatgat cacgacaggt
    15541 gattactacg gtacgctatg gggtgacgga cggctctgga cgagcggcag cgcgccgacc
    15601 ggcgcgggca tcacgcttga tacgggcagc ggcagtccgc agatccgcat ggacgaaacg
    15661 aaccgcatgg caggaaggat cacctcaacg ttcggcacca tgcctgcgga aggcgggcag
    15721 ttcctcttct cggtcgtcaa caacggattc cggcatacgg acacgcttgc gccgcagggc
    15781 atattcggaa acaacttcca cctcaccacc aacaccttca cgttcgacaa caacgggaac
    15841 gacgcgcaga tccgcatcga ggacggcaca ggcggaaacg gcggcacggc gcgaaaaccg
    15901 taccgttacc tctttgacgt tcccgttgtc cttgccgaca atctcgcgat ccgtcatctt
    15961 ttcacgggca cgaaccaata tgggagttac acaaccagtc agggcgacgg tccggcggcc
    16021 ggggtgatct tctcccggaa aatctccggc gggggaaagg gcatcaccat cgagaatact
    16081 gcggccacgt tgggggtgat cttcatgggg gacagcgagt tcacggggga tctcacggta
    16141 aaccgcggtc tcgcccgcgc caaagacacc tcgaatctgc cgcgggtggg cacgcctttc
    16201 ggccaggcga acagcgtcat cgcgacaaac gatgaggcgg gtgtggatct tggcggcttc
    16261 atcacgggtg cggggcagac cctgcgcctg aaaggcaagc gcacctgttt gggggggaca
    16321 aacggggtgc tcttctccac ttgggcgcgc ccgggagacc tctccgaatg gcagggcaac
    16381 gtgatcctcg aaggggagac ctggatcggc ggcacgttga ccgggaacaa ctggcccgcc
    16441 aatttctatc cgatcgacac ggacggcggt ctcgcgatct cgggcccgat ttcagaagcg
    16501 ggcgggtcgg cgacgctcca tgtgttgaac ggacgggaca cggtctttag cggcgccaat
    16561 acattcagcg gtggacttta ccttgataag gggttcttca cggcacgcgg cgcggaggcg
    16621 ttcggcaccg gcggcatcta cttcaacggc ggcatctacc gcgtcacctc tgctgaggac
    16681 gccgacattt tcacggccga cgtgcatggc gcggcgggcg ggttccgcgt ttttctggac
    16741 gcgggcgtga catgcaccat ggcctccaac gcggtcagcg gccttgtcaa accggctttc
    16801 tataaatacg gtcccggcac gcttgtgctc accaaccggg tcagcacggg gacttcgctt
    16861 tgtatcatgg gcggcacggt tgtgctcgac acgacccgtg aagggaacgt gaagttcggt
    16921 ccaaacaata acaactggga gatggatcac gtctatcttt acaacgacgc aacctttgtt
    16981 gtgcggggtg accctgcgac cgtgcggacc ggcgcttggt acgggacgcc gcaggcgggc
    17041 ctctccggaa cgttccgcgc ggaagacgga cagcgtttca acctcgcttt caacgccgac
    17101 aataacctgc ttggctgcgt caacctcgaa accgacaaca gcggggccta ctttacgggt
    17161 gcgggttcgg ctggcagcca cacgtacaac agcgactacg cattcctcgt gtacgagggc
    17221 tcatcatggg tctacacgag cggcggacgc gccgtgccgc tgccggacag cgcgtatcag
    17281 acgacgtggg gcggcaagac cgacatcgtt gacgtgacgc cggcgctcgc cgcagtgccc
    17341 gtgccggcca gtacggaggc ggacgtgatc cgcttcaaca cgccgaacgg cggagccgag
    17401 atcgtgctca cgcttgcggg cgatctcacg ctcgatggcc gcacgatcct tgtgacgccc
    17461 gcgatgggga acacgcccgt gcggatcacg ggcggcgcga tcaagagttc cggcctcatc
    17521 cgcatcctca acttcaacac gaacgcgacc ctgacgatcg aaagcgacct cgcgaagaac
    17581 gttgccaaca cacaactcat cacgggcggt cccggcaaga ccttcctcaa gggcgcgaag
    17641 accttcaacg gtctggtcta cgtggtgggc ggcgagttgg aggtggactg tgcggacgcg
    17701 ctcggcacgg cgaacgcggc gtggaacagc gggtacgtgt ttctttcaaa cggcggcgtc
    17761 ctttcattca acggcgtaat caatccggtc ttgagcgcga ccacgcgcac agagttcagc
    17821 ctccgggttt cgtcgaccgg cggcggcctc aaggttccgg acgcggacga cgtgctcacc
    17881 ctttcagaaa acggaatcaa cgtctgcggc ggcggcctct tccgaaaaac gggcgcaggc
    17941 accctcgagt tgaagaatag cgctaaaatg tcgactcaag acgaaggagg ttacaaaccc
    18001 aacttcgcgc ggaccgattt cgcggaggga acggttatcg ccgctacggg cttccaagag
    18061 ggagcggaga cgatgaccgt caggaacggg gtcagtctga agggcccgcg gtggatcgcg
    18121 ccgagtcagg ctggagcgaa cggcggcggc aaccgcatgg aactgcaagc caagcgccag
    18181 ctcgtggcgg gtgctggcgg ggcgaccgtg gatctgcagg ggcaaaacgt gtcgctcgcc
    18241 gacagcagaa gtttttacgg tctcgacaag agcaccgagt tctttggcgg cacgggcgag
    18301 atccgggtgg tcaacacgag cgcgacggaa gccaagattc agtgctatgg ctaccacgac
    18361 gccgccttcc gcggcaagtt cacgtcccat gtgccggtca cgggtgagca gggtctgggg
    18421 tttcgcaacg ccgaatggtg tgtgccggaa ggcgtgacca acacgttcac ccagattgcc
    18481 gaggaccgtt tcatgttgtg gttcggacgc ctttccggaa agggaacgct caccgcgcag
    18541 gcgattcaga cttacattcc ggggcggttt ttcctgggcc aggatgatgt gccggcagcc
    18601 gattttgagg gtacgctggg cgtgtactgt ggagggaatt cgatcggtgc gctccagctg
    18661 gtgaaggtgg ggaataacgc gcagcgcatt tcgggcgcgg cgaatacgtt cgagggccat
    18721 acaatcatcc gcgcgggatc gcttcttgtg gggaacaact cgcccgcgac ggccggcgct
    18781 tccggcgcac tgggtcaggc gatggtgtat gtgggcgatg cggagacgcc ggcaggggct
    18841 tcgccagcgc ttctggcgga cggcgcgtac acgatcgcga acgaaatccg cgtgcacgat
    18901 ttttcgcccg cgtcggcgac gccggtgctc ggcggcacgg cgaacgcgga cggcgccctg
    18961 ttctcggggg cggtgaggct ctatcgcgat gtcatcttcc gagccgagac agggacgacc
    19021 gtgacgttta cgggagagat cgaggggctg aagggcgccg ggatgtcggg cgggggccgt
    19081 gtggtcttgg cggatgacgt cacgctcggc ggcggattcg tctggacggg cggcacgctt
    19141 caggtaaacg gtgccttcac gattcccgag ggcgcggcgc tcacggtgga cgcttccgtc
    19201 tgcacaaagg agaaccgcgg cacgatcttc acgctgatcc gggcgaatgg cggtatcttc
    19261 ggaacgttca tgttcgcgca ggaacttccc gccggttggc gcgtcgcaac ccgcgcgggt
    19321 gcggtgtgcc tgctttttga cgccggaaca acactctttt tgaagtaacc cgaaaaagaa
    19381 caagggaggg atgaccatga agagacacac gcatcgctgc tggggatccg tgctggccgc
    19441 gggatttacg gcatttgccg ttcaggctgt tgacatcacg tttcccaata ccgacggcaa
    19501 gggcgacctc gcgtcgaccg cgtcgtggaa ccagaccccg attcccgacc cgaccaatcg
    19561 gccggaattc aaggccacgc tcatgggggc cacgctcacg gctagcgaag acatcagctt
    19621 cgcagggttc aaaatcaatg tgaacaatac cgcgtatccg gtgattttgg acatgcgcaa
    19681 cgaggcgacg ggcgcgaatc ccggtcccag aaagatcacg ctgtcggaca acattcggaa
    19741 cgtgagcggc aacaacatca cttacatcaa gggcggcgaa tggaccggca cgtcgatttc
    19801 cacggattgg gggaaatcca ctgacatcta ttatgtccaa ggtgcggtca tgacactttc
    19861 cgggggcctt gccggcacgt acggcgggaa tggtgccctg atcgaactct ccggcggttc
    19921 ggtggtgacc gcgctgcacg cgcgtgcgca atacaattac ggggacaact ccagcattca
    19981 ggtgctcgac ggttcgaagc ttgttgtcac cggggtggcg gatgagtcgt tcatgtccga
    20041 caagtcttcg ggcttcaaca acggctacgt cgtgcgcggc gagaattcac tgatcaaaaa
    20101 aacctcttca gcgaaagccg cattgcttgg tgccggcgga cactcggcct atctgcgggt
    20161 ggaggatcgc ggcgtggcgg actttaccgg gacgctgaga gtgggcaatg cggcaaattc
    20221 gacgaacaac gtcgtgtcgg tcacgggcgg gggcacactc aaggcggatg gggtccaatt
    20281 gggatattcg agcagcgcag ggaataacct tttcgcaatc acgaacggcg gtgtggcgga
    20341 ggttccgaac ggtttctttg tcgggggtaa cgcctatgaa gggtgtttcg ggaacaccct
    20401 tctggtgtca aacggggttt tccgcggaaa agtgccgacg atcggtttca aggcgggctc
    20461 ctccaataac cttgtgcgca ttgtgggacg gcagagcgtg tacgaggcgg ttgccaacgt
    20521 atcttcggat acggcgtgca ttttcgcata cgaaaggaac aaccgcttcg aactcgatga
    20581 tgcggtctgg acgaacaatg tcgcgaagat cacgtatctg aattgctccc agttcacgga
    20641 aggccactgc gtaatggaac tcaggaacaa cgccgaattc tacacgacca accagttcta
    20701 catgaccggc atccactacc gcagcctgtc gaacctcctg tcgatcgtgt ccggttcgcg
    20761 gctctactca aaggggagcg tcggcgtgta ttcgatcggg aacgtgtttt cgatttcaaa
    20821 cggcacgctg gagaccgaag ggagcttcac cgttacggcg cagagcggtt cccccatgat
    20881 gtacggatat tcgaacaact gggtgagggt cgcgggcgaa cggccgcgga tttccgtcac
    20941 gaacggcacg ttcgctgttc agtacgcttc ctatctacag tttgacattc cggcgtccgg
    21001 ctacgaggcg ggtgccgcgc ccgtgaaggc aaaggctttc acggtgtcgt ccgattcgtc
    21061 gctgatcgtg aacggcctcg acgcgcgcat caaagccata aaagagccgg aggttgtggc
    21121 gctcgtcgag tccctcaatg gcgtgacggt tcctgcggga gttctggcga cagcgaatgc
    21181 gggcttgccg aagggatgcg cgcttttcgt ctcggccgac aacaaggcac tcttgttgag
    21241 ggtctataaa gagacgggga ctttgttgag cattcactaa tcggagtacc gcatgatgtc
    21301 attattcgca cttccggttc ttgccgcggc gttgagcgcc gtgacatcga acccctccaa
    21361 caattttttt gtcgccgggc atgaggcgaa ggcgacgctg acggcgaagg ggcttgccgc
    21421 cggcgagaag cggccggtcc ggttggaggt ttacgatttc acctacacga accgtgtggc
    21481 gacggtgccg ggggaaatcg tggcggacga gaaaggcgaa tggaggggcg agttcaccct
    21541 tccgtccgac cggtatggcg tgtatctcgt ccggcctgtc gccgccgggc tcgaactccc
    21601 gaaacggggg accgtgccga aaggcgcgtt cacatacgcg gttctcgcgg atccgtccga
    21661 gatgccggag atcgacctgt gggacacgtt cttaggactg cacggcggca gttattcgtg
    21721 gatgtaccag cgcggcgggt tgggcgacgc gcggaaaccg tctgaggatc gcttcatcgt
    21781 ggcgaacctc gacctgttta acagagagaa agatttctgg aagagcttcc tcacgaacga
    21841 agctgtgcag gcaaaatacc gtgcggacgt gaccggatac gtgaaggcgg ctgtggccgc
    21901 gggcggcgga aagcagaagc gccgtttcta cgagccgaca tgggaggcga atctcaagac
    21961 tccgaacgcg gagacgctgg tgaaagtgca ccagatcgcg tgggaggtga ttcacgaact
    22021 cgacccggac gcttacgtgg gcgcgccgac ttcggcgggg atcaacgacc ttgcgttctt
    22081 gcggtcgctc ttcgatctgg ggctcgccaa gtacatgaac gcgttcgcgg tgcatcctta
    22141 tatcgcggcg ccgccggagg cgaacgggct cgtggatacg gtccgttcgc tcaagcgcat
    22201 gctccgcgag tacatggggc gggacgttcc gatgatcggc acggagagcg ggctcaatga
    22261 gcccaactcg ccgctcggcg aaaagacgaa actcacgggg cagctccgtc agaacctcat
    22321 ccttttcggc gagggttttc tctggaacct gccgttctac ggttacgatt tcggcgcgga
    22381 tgcgaacaat caggatgagg gcgactacgg tgtatgctac aacccgctct atccgaagca
    22441 caggtggggg ccgggggaag tgcagccgcg gcctgtcttc ggcgcgcttg cggcgttcgg
    22501 gcgtctgacg gaagggcatc gtccgacatg tccgatcgaa tggctgggcg agaccgtgct
    22561 cgggtacgcc tttgcggaca aaagcgattc gcactgcgtg atcgcgctct gggactgggg
    22621 cgaacgcgga acagaggtcg agcttcctgt cgggcgcgac gcaatcgatg tggcggacgt
    22681 gatgggcaac gtgacgcgga agaagacgac cggcggtacc ctgaaactca ccctttccga
    22741 atatccgcaa tatgttctgg atgcgaattc cgcaatctgg ggcaaggcgg cgcaggcgaa
    22801 actcaaatgg ggcgaacgga aatacatgtc ggattcggac cgcgcccccg tggggattgc
    22861 cgacctcgcg ccgtcattcg cgtccggcgg ccccggcgtg gccgtcacgc tcgagaaccg
    22921 cacggacgaa ccgcataggg tcgcgctgga aacgcggatt ccgggcgaac ccgacgcgcg
    22981 ggtgaagcgg agcgtttcac tcgcggcgcg gcagacgcaa cgcatcgaaa tcgcattcga
    23041 cggtttcacg cctgatccga cagtgttctt tgacgtcgag gtgcgcgtcg tgccggagaa
    23101 ggggatggtg gcgacggcgg tggactcgtt caacttcctt cccgtcccct cttcgttcgc
    23161 gtttgacggc ggcaagacgg tgtcgctcgg ctatgacgcg cggttcctga cgttcgacat
    23221 cttcatcccc gactcgacgc ccacaaacgg ctggacgggg tgggacagtt ggaagggcga
    23281 cagcgtgcag gtggggctcg cgaaaagcgc gctcaaaaaa cgcacgcaga acgatctcgc
    23341 ggacaatgcg gccgaggcga aaagcgaaat cacgttcgcc ctgacaccca acggccccga
    23401 aatgtatcgc accatcacgt gggatgtgag gcgcttcccg tgcgacaccc atctcgcggg
    23461 ccagatcgac ttgaaggaag ccccgttgac ggtggagaag tccgcgaagg ggttccggta
    23521 ccgcgcgtcc gtcccgtggg cattcctcaa tctgtccgca ccgaagccgg gcgacacgtt
    23581 ccggttcgcg ttccaattca acgatcggga acccggcaat cgttttctga aggaatcgaa
    23641 ggggttcgaa ctcaaacagg ccgcgccgaa aaagttcggc tgggggacga tccgatgacg
    23701 gctctgctgt tggcggcgtc gcttcagttc cacggggtca tggggcagag tgcggcgaaa
    23761 ggggaagcgc cgcttgaatt caccggtgtc tcctttctgt cggagggccc ctgcggacgg
    23821 gtgtttttcg gaggcgacga cgggaaactg tacgagttga aggacggaga gatcatccgg
    23881 acaggcgtgc ggatgcaggg ccgctttctc gactgggacg gcaagactgt tcgtgcgctc
    23941 ggtcagcacg atgtctggga ggtcgacccg gcgacactcg cgttcaaaca cgtgatcagg
    24001 acgcgggatt tccgtttcga ccttgcggcc gtggtgccgg acgatcccgt gcacccgttc
    24061 accgcgaagg gcaaatacgt cacgtacgac aaaaaacgcg acgcgcttct cgtgttggac
    24121 gagaaaggtg ccgaacgcgg tgggctcttc ccgctccccg aacgactttc gaagagtgaa
    24181 atcatcagcc tcgggttctt gcccgattcc ggcgatctcg ttgccgtgtc gtattatccc
    24241 gatttgcgta tctatcggtt cacggcggaa ggcacgcagg tgacgggaca cggttggccg
    24301 gcgcggcgcg ggttcgggtt cttccggaaa tccggcggcg aactctatca ctgcggcacg
    24361 ccgacgctcg tgcggatgga ggaaaacctc gcgggtgcac aagggaacgt cttccgtgtc
    24421 gggaaggaac atttcacgtg cggttatgcg aaacaagggg accgggagta catcgggacg
    24481 agccagggac tctatgtgaa agagccggga gagacggaat tccggaaacg gttgggcggc
    24541 atacgcccgt tgacggctct cgcggtgaat ggcaagtacg tgtacatgtc gatgggcgga
    24601 acgatccggt ggatgtactt agacgaggac gaaaacgggc cgttcgcctc ttccgatgac
    24661 cacatcttcc gggtgaacgg cggctggacg gaaaagccgt acgatttgat cccggacggg
    24721 gacaacctgc tcgttgccgc cggcaaactc ggaacatggc gcttcaagcc ttttctgccg
    24781 ccggcgtatg cctttaaccg gaagcattgg gaacaggtct ctacgaatgt ctgcacggaa
    24841 ctttccgtcg ggaaaccttc gtgcgagcta ctggggttcc tttcttctgc agaggttccg
    24901 gacggcctgg aacccggcaa gatcgcaagt cagggaaagt ggcttgtcgt tgaggatcgg
    24961 aagaacttcc gtctggtgag gttcagaatt gacgctgaca agaagtaacg aagcgacttt
    25021 gatgttgtcg atcacttcaa gtgatcctgt ttttatcgca ttctgccggt gttttcttta
    25081 ttttcttctt tcaacgggtt caggaagggg acggcagacc atgcggacac agacggttgc
    25141 ggtcgggatt ctggcgggaa ttctgtggtc agcgggattg cgtgcccaag aggtgctggg
    25201 gccaatgggg ggtgtgggcg gcgaggcccg cagggttgag cagcag
//
LOCUS       DAOSLE010000019        24291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2304_length_24291_cov_7.741583, whole genome shotgun sequence.
ACCESSION   DAOSLE010000019 DAOSLE010000000
VERSION     DAOSLE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24291
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_24291_cov_7.741583"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK576_02675"
     CDS             complement(<1..407)
                     /locus_tag="PK576_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60524.1"
                     /translation="MRRGELTGRWDTNFLLNRTDFSQFRYEIIPIENICLDVFQGITI
                     KKTDDVDAPKVLKVKNICLNGSIDFCEIESTTNVPDRKLLKVGDVISPFIGEAIRQHK
                     VALYEDEANKYTVDGNVGVYRPNKKKIKPRFLKA"
     gene            complement(443..2509)
                     /locus_tag="PK576_02680"
     CDS             complement(443..2509)
                     /locus_tag="PK576_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE60525.1"
                     /translation="MSTSTQNFLQRAFADGHLQLITSGKTEKIRYATVNVSEKWSDPE
                     EKVRAAYYAELIYRYGYAPEYIGVEVTVPDRTPVDRADLVVFRDKEHTKPFGVIECKR
                     DGISDAAFRQAVEQAFGNGHAHKFRAEYIGVIAGQTRAFYDCSDKFGVLEREANIVAD
                     LPIQYGKPEEFKYYKDAFPKPDISAVSRDDLIKTIRKCHQTLWGGGRLSPPMAFGELT
                     KLIFLKTRDEKTPRKKGEPYKFQIKTNETAQRLATRIKDMYAIERQKEPDVFNDDIKI
                     DDVTLKNVVLHLEGINLTATDLDTKGVAFEQFMDGFFKGDFGQYFTPRNIIALTIQML
                     DIRADDFVIDPACGSGGFLLHALDHVRRQACQYYEADSKEHFDYWHSFAEKRLFGIEI
                     NEEIARVAKMNMIVHDDGHTNVIGNDALEPLINIGRKNASFAAIMGLNLQTGKRDESK
                     GFSKIPTNPPFGAVIKEELHSYLPSYELSRYISKSGGKDEDDAENTDADYKAGKKAVK
                     QRSSVKTEIIYCERIWQLLKPGGRAGVVLPDGLLTNASLQGVRDWILERFKVLAVVSL
                     PQFAFAHFGAGVKSSVVFLEKRQPNVPASDGEAIFMAIAENVGYDATGRSTYQVEMVD
                     EKPGLLRTERQRCDLFDWEVTFNWVPGEGKSPGHWSEQHRHVVPGSGLLGQYAAFKRN
                     PDPFFV"
     gene            complement(2753..4504)
                     /locus_tag="PK576_02685"
     CDS             complement(2753..4504)
                     /locus_tag="PK576_02685"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE60526.1"
                     /translation="MHTEHPSGQRGRRPRKPFRRRNRHPGLAEREFKPTQVPKPKGTI
                     PEAFAGTVFGLVHPLLQHAVADEAYLTPTPIQEKAIPHLLDGRDLMGCAQTGTGKTAA
                     FLLPILHRLNLNPKPVERNRPRALVLAPTRELAAQIATSTATYGRYLGLPFAVVFGGV
                     SQYPQVNALNRGAVLVVATPGRLLDLHGQGHIALDGIEEFVLDEADRMLDMGFMPDIK
                     RILAMLPEARHTQFFSATFSPEITRLAMSMVKDPVQITISPDQPTVDRINQTLMFVDK
                     ANKDALLGEILATRPEMSKVLVFARTRHGADKIERKLCRAEISARAIHSDKTQRARMD
                     ALDAFRHGRVRVLVATDIASRGIDVDGITHVVNFDLPEETESYVHRIGRTARAGAAGV
                     ALSFVSAEERGLLRAIERLIRKSIEVDNTHSYHSERAEKAVGKGRDSLPRPAWASRIR
                     TQGGAKGRGAYSRDRGPGRGPQGRRQPDFRRGPGAKPIPSDAPDRRPARAAQSREKPA
                     VETERRDRRRPEGKRFPAAGPAAPRPARGGDEAKKDQGRKPRGGGSGGFKSGGFKPRS
                     RKGGKPKHRPARPPWMT"
     gene            4499..5461
                     /locus_tag="PK576_02690"
     CDS             4499..5461
                     /locus_tag="PK576_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE60527.1"
                     /translation="MHVAFVYTLSARPSKVKTPAVYSRRAGRPVLFYIVIGRILLAWG
                     VLMLIDVHTHVFPDHLAARALATLTAKTGNAIAPVADGTLSALLENLQRDGVDRAVVC
                     PIATRPDLFEGLLREALALRDGERGKDAARRLIPFASVHPDDEARFAHLTRVAESGLI
                     GIKLHPYYQPCVLDAPPMLEFFRCCRDLGLVVQCHCGFDIGFPFEPVCGPARVARVMR
                     EVPGLRFIASHLGGWRQWEESVTHLLGTDIYLDTAVLRQDREDPLLLRLLREHPTDRL
                     LFATDWPWLGFADGIAFVRSAALGAKAERAILGGNAIRLLGIPA"
     gene            complement(5469..6881)
                     /locus_tag="PK576_02695"
     CDS             complement(5469..6881)
                     /locus_tag="PK576_02695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE60528.1"
                     /translation="MIGENEKAEQVAQQAQNFYNKGVAAFERANLDIAIDLLMQCLIL
                     APGFSRARKFLRAAQIAKFRKAKKNPLSSKIQEVAAFFQRLKVTGLMKAGKHEAALIE
                     CEKLLIMNPLHSENVELAVEVAEAAGHPEAALFTVEAAYDNNPDDMTLLRRVADYYMA
                     VGDYDKAREAYLKLNAYKPNDQTIFKLLKDAEARMTMVSGGWEEVAGQKDGYRNLIAN
                     KEQAKKLDIQAKAVVAGTDAESLIEEARGRIEKEPNNLNLYRALARLYTQNKRFDEAV
                     QTLEAAREVNPADPELDRALTATRISAFEARIEALQTAGDAEAAAAAETEMNQFIFDD
                     LASRVQRYPNDLKLRFELGLQYYKYDYFDEAIGQLQLAQRSPKERVEALYYLAKCFAK
                     KGQRDMAIMQLETANDQLPIMDDLKKRVVFELGVLAEESGDIEKAFGYYKDVYGADIG
                     FEDIGARMERIYKLRQSQQG"
     gene            complement(6891..7610)
                     /locus_tag="PK576_02700"
     CDS             complement(6891..7610)
                     /locus_tag="PK576_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE60529.1"
                     /translation="MRRWVWLGALAIGFGLAAAVGVRGLRDTRPVLVCLGDSLTSCGG
                     QGGRYSDWLARFMLYVRVIDAGVGGDTLMGGRARFERDVLVHNPDILVIALGANDFWQ
                     RNRPPEALAADLEAMVREARSRSMQVVVASCFGDRFFWEEPCVEFTPDRFALAREIAR
                     LERRVCETHGAEYVPNMQIDIKPNRLPPYWDKTDHPNRAGNEQVALRLLPAIRRAVEL
                     DGAKVSPYSEKANSQQGYDKK"
     gene            7865..8041
                     /locus_tag="PK576_02705"
     CDS             7865..8041
                     /locus_tag="PK576_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60530.1"
                     /translation="MKIRNFPLAFQKNPSDAIPCVRGEIIDSDSDSDPDSDSDPDPDS
                     DPDPDLDETMKPET"
     gene            8426..9370
                     /locus_tag="PK576_02710"
     CDS             8426..9370
                     /locus_tag="PK576_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOE60531.1"
                     /translation="MFPSENQTQPDPAACRPRAAAALLPWLTVPRFWSLTASTVPVTV
                     GAALAAHDGHFNGPLLALCLLSGWLLQIAANLLNTYGDFRSGVDTSAHPPTAPHLVNG
                     TLRPYHVLGAGVLCLLAGALAGARAAALSDWHLALFAVAGVAGAACYTTGPRFKYAGF
                     GLPVVALLMGVLMVTASYFAQTRTLTLPSLILSLPVTCLVGAILHGNDLRDMASDHRA
                     GIRTASLVLGSERAPPLFMALHILPHLILVAGIAGGLLPPLSGIALLALPFTLNALRT
                     CAIGFRTGDRTRIGRLEGLSAGIHFLFGTLLTLGLFLG"
     gene            9382..9999
                     /locus_tag="PK576_02715"
     CDS             9382..9999
                     /locus_tag="PK576_02715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOE60532.1"
                     /translation="MRATHRNFWLLTALAAIGFLWVFGCPAGNFWGKITLTVCGLCTA
                     SIVVAGVPWRHTDLTLAEGLIGVAAAVALYGLFWAGYALACRLFDFAPQQVNAIYAIR
                     GAGHPWLIGALLLLVTSPGEELFWRGFVQRQAAKRWGATTGVVLGTTFYGAVHLVSGN
                     LMLVLAASVAGLFWCALYRWRGNLTACMVSHALWTVGIFLFFPIR"
     gene            10165..10494
                     /gene="trxA"
                     /locus_tag="PK576_02720"
     CDS             10165..10494
                     /gene="trxA"
                     /locus_tag="PK576_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOE60533.1"
                     /translation="MAGTVLDLTDATFDGAVAQGVTLVDFWAPWCGPCKMQTPILETQ
                     VAPALAGRANVAKVNVDDAKELAVRFSIKSIPALLIFKDGQVVQQMVGLQRGDALVKA
                     VEAVLGA"
     gene            10593..12530
                     /locus_tag="PK576_02725"
     CDS             10593..12530
                     /locus_tag="PK576_02725"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOE60534.1"
                     /translation="MTDYSKLLNPEQCEAAMAGEGPLLVLAAAGTGKTRTLVYRVAYL
                     VEQGQPPDGLLLLTFTNRAAREMLDRAKEVAGETVGNLWSGTFHHICNRFLRRFGDRL
                     GYPHDFTILDRDDSHSLIDQCIRETVRNRKEFPKKDVIASLIGRAANRACDIEDVLDS
                     TLTELRVDPTEILRVANLYAEKKRGMGAMDFDDLLVNGLRLLTEHEAVRVFYQSRFRH
                     VLVDEYQDTNLLQARMVDILADTHGNIMAVGDDFQCIYSWRGADFRNIMDFPKRWPTC
                     RMVKLERNYRSVPEVLEVANACIRGNPEQFQKTLRPTRKARNKPQVLFLRDGEEQAAA
                     LLTLIERYQEDGYRLRDMAVLYRAHYHSIELQIALGRARIPYVITSGIGVFEQAHVKD
                     VLAFLRVCVNRSDRLAFGRLLSLLDGVGEKTVESLWSKIGNTFDTQSPETRARLRAAM
                     RPAVREQWAAIDQLFQAYHSEKLNTAGGEIVQRFCDLFYHTLLYRRFDNADKREEDIR
                     ELALQIGKSPSVEAFLQEVALLTNVDHEYDGAEREAKERLHLSTVHQAKGLEWPVVFV
                     IWVTEGMFPSSRSVGEAENDAEERRLFYVAVTRAKDELVLCAPGMRRTRDGGVFFCKP
                     SRFIKEIPRGLTRERYGNRYV"
     gene            complement(12544..13341)
                     /locus_tag="PK576_02730"
     CDS             complement(12544..13341)
                     /locus_tag="PK576_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60535.1"
                     /translation="MRNGVLAAALWIGLAGGALSQEAAPVAVGEAETAGFSFGLTLDL
                     FSAYVWRGCVYNDEPVFQPAATLAYATERYGTFGLGLWGNLDLTDRNRKVSGGSFNEV
                     DYSLFHAFDVGGFTLEWGHFGYTFLKANGRDSYGNTKEVYLSVSHNNDLVTPFASLAY
                     DYDLLDGFYFNAGLNKEVALGDQLALGAEVSVGAGDDDYMTYVGVDEPGLMDFNFSVY
                     AIYALTDTLSVSAKLAWMSLLDGDARDAEPYRDEDLLWGGVSLNLAL"
     gene            complement(13372..13986)
                     /locus_tag="PK576_02735"
     CDS             complement(13372..13986)
                     /locus_tag="PK576_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOE60536.1"
                     /translation="MRVPPRSDPVRWRLKRRAISRIMREYEQKRAALGNPVSPTLFRN
                     PKFYFAAIVVFVFLGILLSNTTDKAVRRMEVTPHMRAIRNLDVLAEALGRYHFHTGSF
                     PDAVPGLAALVRDPAVPKWDGPYIRFLRNDPWNTPFVYAPPAGNGLPTLFSCGEDRIP
                     HTPDDLFPDPARFDPGTAWTNGWVSAEQRLPGVTVLPSSPDGKR"
     gene            complement(14237..14650)
                     /locus_tag="PK576_02740"
     CDS             complement(14237..14650)
                     /locus_tag="PK576_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOE60537.1"
                     /translation="MSQELEEGATLTLDFAKIAKIAETCKDAIPVAVQHADTREVILV
                     AYTNELAMRKAFAERTLVLWSTSRNTLWEKGRTSGETFDLLEARVNCEQNSLLYIVRP
                     RRGAICHTRNRNGEPRNCYYRRIDLDTLALTNLDP"
     gene            complement(14672..16336)
                     /locus_tag="PK576_02745"
     CDS             complement(14672..16336)
                     /locus_tag="PK576_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOE60538.1"
                     /translation="MHTITVTVKGQEPMVVAVGTRISELLPSVDRDGFPVLGAVVNNM
                     VQPLFMSFITDAKVEPLTIRDPRGWAIYRASLCFLLAKTVHELFPDVGCRVRNSIGTG
                     LYCTVEWEECSEDALRDQVARLKQAMLETVRQDLTISTETVSYETAVKRFRETGQTDK
                     LNLLAHRNPPVICLTRCGTFFELSQEPLVSRTGVLNLFDLTPLSCGFVLTVPTPDSPS
                     ALPPLPPFEHLFRVYREHIEWGRIVGITTVGELNQAVIEKRVDDFIQTVEALHDKKLG
                     RIADEITQREPSVRLVLVAGPSSAGKTTFSKRLITHLRVNGYRPLLISTDNYFVGDAR
                     NPRDAEGRLDYEHIEAMDLQRLNRDLLRLLAREEVHLRGFDFKTKEGYDRPETTRLPD
                     NGIIVMEGIHSLNPQLIADVPRDRTFRIYINALTQLGVDSSNRISTTDTRIIRRMVRD
                     HRFRNRPAIETLRMWPSIVRGEQRWIYPFEHLADAVFNSALDYELAVLKPLIAPLLNQ
                     IKPWDEVYIEARRLSGFLHNFTPLSPDVVPGDSILREYIGGSQLTY"
     gene            complement(16318..17280)
                     /locus_tag="PK576_02750"
     CDS             complement(16318..17280)
                     /locus_tag="PK576_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60539.1"
                     /translation="MRQQCGSRPDSGRETHGAAIFVYWQRTELIIYLDTTGGEPLSKR
                     GYRITPGTAPLQETLAAGCVLASGWDANSPFVVPMCGSGTPAIEAALLALNRAPGSFK
                     SHFGFMAIKGYRITIPGERAGASVRQRFGASPEQIWKDMVATARDQERREGLPPILAT
                     DIDPETLESARLNAIAAGVSEHITFAACDFEATQLPPPPGVIFLNPPYGQRLASGVAV
                     ESTAETDAESAADEPASGEALEPLYRRIGDFFKRQAVGYTGCVLTGNLELSRRIGLRS
                     SRRIPFFNGPIECRMLVFDLFEGTLKEAKKGNNPEQEASCTPLP"
     gene            complement(17758..19185)
                     /locus_tag="PK576_02755"
     CDS             complement(17758..19185)
                     /locus_tag="PK576_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60540.1"
                     /translation="MMQFNRRDFIRGSALAAASLVAGGCETTGAAGRYPAVTRTVAPR
                     FRKPGEKLNIGVVGVGGKGWSDWMPMFKNGENIVALCDVDRNPINKALAELEKAGVKP
                     SQVKCYSDFRKMLDECKNLDAVTVSTPDHMHAPAAIRAMKQGCHVFVQKPLVRTIWEA
                     RYFEKVAKSCGVVTQMGNQGSANNGLRRNAELLQAGILGDVTEVHVWTNRPIWPQGIG
                     RPEGSDPIPETLDWDSYLGTAPVRPFKAGVYHTFKWRGFFDFGTGAFGDMACHTMNLP
                     FRGLRLGAVESAECIQIEGKNNETYPNKSIVRLTYAARGRLPRVTLYWYDGNLKPSAE
                     IMPKVVATLGQVPNTGCLIMGSKGMVCSTNDYGADGYIAFNDEAKMKSMSKHEALTEA
                     VIPAKFRPNTNGHYLEFVNACKGQDVCFSDVDHSVPMLESMLVGCVAQQVPGKLKWDS
                     RKQEFANNAAANALVKPHIRPGWEF"
     gene            19314..20333
                     /locus_tag="PK576_02760"
     CDS             19314..20333
                     /locus_tag="PK576_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOE60541.1"
                     /translation="MNQKDRAFLAARVGRMEQMAAIRRLVEEDGRGRGMRVLDFESGS
                     GLAFSVYPDRGMDIGPARYKGIPLAWLACQPPAVPHAYDPEGCEWLRTWGGGLLTGCG
                     LLNVGGPCAADGERHGLHGRLSHLAAEEVNTSAAWTPDGTYTLSASGRMRHARVFGEN
                     LTLTRTISTAFDTPSITVRDTIENLGFAPTPVMLLYHMNLGWPLVDPEATLVMPDHPV
                     TPQNEHAAAGLAEWQRFAAPAPGFREQVYYHTLPADEQGLASAALVNPQLRLSLRVTY
                     RVAELPFLIQWKMCGQGEYVLGLEPANCYPEGREAAAQRDLLRTLEPGERLETFLRLS
                     VEPLA"
     gene            20339..22087
                     /locus_tag="PK576_02765"
     CDS             20339..22087
                     /locus_tag="PK576_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOE60542.1"
                     /translation="MPLPCTQREIRQLGWDAPDVILVSGDAYIDSPYSGVAVIGRVLE
                     REGFRTAILSQPDPASTEAFRSLGEPRLFWGVTSGCVDSMVANYTAAGRRRRQDDFTP
                     GGVNNARPDRAVIVYCNQIRAAFKPCKPIVIGGIEASLRRIAHYDCWSDTVRRPLLFD
                     AKADALVYGMGERTITALARAWRDGTDWRGLRGLCHALPASSQARPANAVALPDFNTV
                     AAPTPEGRSAFLEMFRLFATNQEAHTASPLLQKVDTRCLVHNPPAPPLTSAELDAVYA
                     LPFTLDPHPAVAARGPIRALDTIRFSLTTHRGCYGACRFCAIAMHQGRQVVSRSQSAI
                     LDEARRMTRHPAFTGILRDVGGPTANMYGFDCSRKTLQGACPDRECLFPACCDSLRPN
                     HGPQRELLASLRRLPGVRKVFVASGIRPDLIAADPRHGDGYIDDLAAHHVSGQIKLAP
                     EHTEPRVLAAMGKPGLDALLTFKRKFEAANRRHGLRQFLTYYFIAAHPGCTDDDMRRL
                     RRFVASHLHLNPEQVQIFTPTPSTWSTAMYYTGYDPFSGTPIHVTRNPRDKQRQKAIL
                     APDAARARNLVITKRG"
     assembly_gap    22348..22447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22593..23306)
                     /gene="rnc"
                     /locus_tag="PK576_02770"
     CDS             complement(22593..23306)
                     /gene="rnc"
                     /locus_tag="PK576_02770"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE60543.1"
                     /translation="MPEGKTDPLDAFEARLGYVFRDRELLRTALTSPSFRAGRVVGAI
                     PDNQRLEFLGDAVLGLLAAQSLYDRYLAEDEGNLTVRRSLQVNRGALANLARRLGLGA
                     CLRIGQREEENGARDQDRMLADALEAVFGAIWREGGWDAVWRVYRRLDPEEEEAVPWR
                     GNPKGALQELAQRHAWPNSPTYELIGRSGPDHGPVYTVRVRVAGGHEAEGEGRTKRAA
                     ETAAAQALLCRLAPPESLV"
     gene            complement(23336..24100)
                     /locus_tag="PK576_02775"
     CDS             complement(23336..24100)
                     /locus_tag="PK576_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOE60544.1"
                     /translation="MMTVTLSALESLLDETLEIGRFRDASNNGLQIANNGSVTRVVAG
                     VDASLRLLEAAAARGADAVVCHHGVSWGDSLKRITGLNHRLVSFALAHNLAVYAAHLP
                     LDAHPRYGNNARLCKALGLRRPVPAFTYHGETIGFVAAYERAMPLERFFKRVQKVVAP
                     ACRLLPFGKRSIRTVGVVSGGAADMIDQAAAHGVDAYLTGESSLQGYNLAEQLGQTVL
                     FAGHYATETFGVRALAELIRRRLGIPAEFIDFQIEY"
BASE COUNT         4466 a         7847 c         7113 g         4765 t
ORIGIN      
        1 gccttaagaa atcgtggttt aatttttttc ttgttaggcc gatatacacc cacgtttcca
       61 tctacagtgt atttgttggc ttcatcctca tacagtgcaa ctttatgttg tctaattgct
      121 tcgccaataa atgggcttat cacatctccg actttcaaca actttctgtc gggaacattt
      181 gtagtcgact caatttcaca aaaatcgata gaaccattaa ggcaaatgtt tttaaccttc
      241 aacacttttg gtgcatccac atcgtctgtt tttttaatag taattccttg aaacacatcc
      301 aaacagatat tttcaatcgg aataatttca taacgaaact gtgaaaagtc tgttcggttc
      361 aataaaaaat tagtgtccca gcgtccggtt aattcccccc gccgcaccgc gaagcagact
      421 ggctgccccg cgggtgcggg ggttagacga aaaaagggtc tggattccgc ttgaaggccg
      481 cgtattgccc gagtagccca ctaccgggga cgacatggcg atgctgctcg gaccagtgcc
      541 cggggctctt accttcgccg ggcacccagt tgaaagtcac ctcccagtcg aacagatcgc
      601 agcgctggcg ctcggtgcgc aatagtcccg gtttttcgtc caccatctcc acttggtagg
      661 tgctgcggcc agtggcatcg tatccgacgt tttcggctat ggccatgaag atggcctcgc
      721 cgtcagaggc gggcacgttc ggctggcgct tctctaagaa aactacgctg gacttcaccc
      781 ccgcgccaaa atgggcgaag gcgaattgcg gcaggctcac cacagccagc accttgaagc
      841 gttccagaat ccagtcgcgc actccttgca ggctagcgtt ggtcaataga ccatcgggca
      901 ggactacgcc agcccgtcca cccggtttca aaagctgcca aatccgctca cagtagatga
      961 tctcagtctt gacactggag cgctgcttga ccgccttctt gcctgccttg taatcagcat
     1021 cggtattttc tgcgtcgtct tcgtccttgc cgccactctt tgaaatatag cgggaaagct
     1081 catagctggg aaggtagctg tgcagttctt ccttgatcac cgcaccaaaa ggaggattgg
     1141 tcgggatctt ggagaagccc ttgctctcgt cgcgcttccc ggtttgaaga ttgagcccca
     1201 tgatggcggc aaagcttgcg ttcttgcgcc cgatgttaat caagggttcc agagcgtcgt
     1261 tgccgatgac gttggtgtgg ccatcgtcgt gaacgatcat gttcatcttg gcgacgcggg
     1321 cgatctcttc gttaatctcg atgccaaaca gccgcttttc ggcaaaactg tgccagtaat
     1381 caaaatgctc cttactatct gcttcgtagt attgacaggc ctgtctgcgg acatggtcga
     1441 gggcatgcaa caagaaaccg ccggaaccac aagccgggtc tataacgaaa tcatcggctc
     1501 ggatatcaag catctggatg gttaaggcga taatgttgcg tggcgtgaag tactggccaa
     1561 agtcaccctt aaaaaaacca tccatgaact gctcgaaggc aacgcccttg gtgtcgaggt
     1621 cagtggcggt gaggttgatg ccctctaagt ggagcaccac atttttcagg gtgacatcat
     1681 cgattttgat gtcgtcgtta aaaacatctg gttctttctg ccgttcgatg gcatacatgt
     1741 ccttgatgcg ggtggcgaga cgctgggctg tttcgttcgt cttgatctga aatttgtaag
     1801 gctcaccctt cttgcgcggt gttttttcgt ctcgagtctt caaaaagatt agcttggtca
     1861 actctccgaa ggccatgggc ggcgagaggc ggccacctcc ccacagggtt tggtggcact
     1921 tgcggatggt cttgatcaaa tcatcacggc tgaccgcact gatatctggc tttggaaagg
     1981 catccttgta gtatttgaat tcctctggct tgccatattg gattggcagg tcggcaacga
     2041 tattcgcctc gcgctccaga acaccaaatt tatcggaaca gtcatagaag gcgcgggttt
     2101 gaccagcgat gacaccgatg tactcggcac ggaatttatg ggcgtgaccg ttaccgaaag
     2161 cttgttccac cgcctgtctg aacgcggcat ctgaaatgcc gtcgcgcttg cactcaatga
     2221 cgccgaaagg cttggtgtgc tccttgtcgc ggaaaaccac gagatcggcg cggtccaccg
     2281 gagtgcggtc gggaacagtc acctcgacac caatgtactc gggcgcataa ccgtagcgat
     2341 agattagttc ggcatagtaa gcggctcgta ctttctcttc cggatcgctc catttctcgg
     2401 aaacattcac cgtagcgtag cggatcttct cggtcttacc gctggtaatg agttgcagat
     2461 ggccgtccgc gaaagcgcgt tgtaagaagt tctgagtgct cgtgctcatc tcaattcccc
     2521 tttgttttgt tgagtccgga agactgctct tctttttttc atacatgctg taaacccgcg
     2581 agggtttcct gcgcggtgtc gagcgcctct tgacgactaa gggacaggta atttgaccat
     2641 ccccaaaaaa ctttgtgccc tttgtgcctt tgtgtgagaa aaaagtgcca tgcgatttgt
     2701 ggccggatcc gtacgaatga aacccgaaac ccaatacgcg caatggggca gatcaggtca
     2761 tccacggtgg acgcgcggga cgatgtttag gcttgccgcc cttccgggag cggggcttga
     2821 agccgccgct cttgaaacct ccgctgccgc caccccgcgg tttccgtccc tgatccttct
     2881 ttgcctcgtc gccgccgcgt gccggacgcg gagccgcggg acccgcagcg ggaaaacgct
     2941 ttccctcggg acggcggcgg tcacggcgtt ccgtctcaac cgccggtttt tcgcgggact
     3001 gcgccgcgcg ggcgggccgc cggtccgggg catcggacgg aatcggcttc gcgccgggac
     3061 cgcgacggaa atccggctgg cgccgacctt gagggccgcg accgggaccc cggtcgcgcg
     3121 agtacgcgcc acggcccttc gcgccgccct gcgtgcggat gcggctcgcc caagcgggcc
     3181 gcggcaggga atccctgcct ttgccaaccg ccttctccgc ccgttcggaa tggtaggagt
     3241 gcgtgttgtc gacctcaata cttttccgga tcagccgttc gatggcccgt aagagaccgc
     3301 gctcttcggc gctgacaaac gagagggcaa cccccgccgc gccagcgcgc gccgtgcgcc
     3361 cgatgcgatg cacgtacgat tcggtctcct ccggcagatc aaaattgacg acatgcgtga
     3421 tgccgtccac gtcaatgccg cgcgaagcga tatccgtcgc gaccagcacg cgcacccggc
     3481 cgtggcggaa agcgtccagt gcgtccatac gcgcccgctg ggtcttgtcg ctgtggatcg
     3541 cgcgcgccga aatctccgcc cggcagagct tgcgctcgat tttgtccgcg ccgtgacgcg
     3601 tccgcgcgaa aaccagcacc ttgctcatct ccggacgggt ggcgaggatc tcgcccagca
     3661 gcgcgtcctt gttggctttg tccacgaaca tcagggtctg gttgatccgg tcgaccgtgg
     3721 gctgatcggg cgagatggtg atctgcaccg gatctttcac catcgacatg gccagccggg
     3781 tgatctccgg tgagaaggtg gcagagaaga actgcgtatg gcgcgcttca ggcagcatcg
     3841 cgagaatgcg cttgatgtcc ggcatgaacc ccatgtccag catgcggtcc gcctcatcca
     3901 gcacgaactc ttcaatgccg tcgagcgcga tgtgcccctg tccgtgcagg tccagcagcc
     3961 gtccgggcgt cgccacgacc agcacggccc cgcggttcaa cgcgttgacc tgcggatatt
     4021 gactgacccc gccgaacacc actgcgaacg gcagcccgag gtaacggccg taggttgcgg
     4081 tactggtggc gatctgcgcg gccagttccc gcgtcggagc gagcacgagc gcccgggggc
     4141 ggttgcgttc aaccggtttg gggttgagat tcagccggtg gaggatcggc agcagaaaag
     4201 cggcggtctt gccggtgccg gtctgcgcgc agcccatgag gtcgcggcca tccagaaggt
     4261 gcgggatggc tttttcctga atcggcgtgg gggtcagata ggcctcatcc gccacggcat
     4321 gttgcaaaag gggatggacc aaaccgaaaa ccgtgcccgc aaaagcttcc gggatcgtgc
     4381 ccttgggttt ggggacctgg gtcggcttga actcgcgttc cgccaggccg ggatgacgat
     4441 tccgacgacg gaaaggttta cggggacggc ggccacgctg tccggatggg tgttctgtat
     4501 gcatgtggcg ttcgtatata ctctttccgc ccgtccaagt aaagtcaaga cccctgcggt
     4561 ttattctcgc cgtgcgggcc gtcccgtatt attctacatc gttattggcc gcatcctgct
     4621 ggcttggggg gttctgatgc taattgatgt ccatacccac gtctttcccg atcatttggc
     4681 cgcgcgggcc cttgccacgc tcaccgcgaa aaccggaaac gcgatcgccc cggtggcgga
     4741 cgggacccta agcgcactgc tggaaaacct ccagcgtgac ggcgtggacc gggccgtggt
     4801 ctgcccgatc gccacgcgcc ccgatctttt cgaggggctg ttgcgggagg ctctggcgct
     4861 gcgcgacggc gaacgcggca aagacgcggc ccgccgcctg atcccgttcg cctcggtgca
     4921 tccggatgac gaagcccgtt tcgcccatct cacccgcgtg gctgaaagcg gcctcatcgg
     4981 aatcaaactg cacccgtact accagccttg cgtgcttgat gcgccgccga tgctggagtt
     5041 tttccgctgt tgccgcgatc tggggcttgt ggtgcagtgc cactgcgggt ttgacatcgg
     5101 gttcccgttc gaaccggtct gcggtcccgc ccgcgtggcg cgcgtgatgc gggaggtccc
     5161 cggcttgcgc ttcatcgcct cgcatctggg cgggtggcgc caatgggagg agtcggtgac
     5221 acacctgctc gggacggaca tctatctgga caccgccgtc ctgcgccagg atcgggagga
     5281 cccgctgctg ctccgcctgc tgcgcgaaca tccgacggac cggctcctgt ttgccaccga
     5341 ctggccttgg ctgggattcg cggacggcat cgccttcgtc cggtcggccg cgctcggcgc
     5401 gaaggccgag cgcgccatcc tgggcggcaa cgcgatccgt ttgctgggga ttccggcgta
     5461 gagccggctc agccctgctg ggactgccgc agcttgtaga tgcgctccat gcgcgcgccg
     5521 atatcctcga aaccgatatc cgcgccgtag acatccttgt agtacccgaa ggccttctcg
     5581 atgtcccccg actcctcggc cagcacaccc aactcgaaaa cgaccctctt tttgagatca
     5641 tccatgatcg gcagctggtc gttggcggtt tcaagctgca tgatcgccat gtcgcgctgg
     5701 ccctttttgg cgaagcactt cgccagataa tagagcgcct cgacacgctc cttgggactg
     5761 cgctgcgcga gctgcagctg gccgatggcc tcgtcgaaat aatcgtactt gtaatactgc
     5821 aagcccagct cgaaacgcag cttgagatca ttcgggtagc gctgcacgcg cgaggccaga
     5881 tcatcaaaga tgaactggtt catctccgtc tcggccgcgg ccgcggcctc ggcgtcgcct
     5941 gcggtctgca acgcctcgat ccgcgcctcg aacgccgaaa tccgcgtggc ggtcagggcc
     6001 cggtccagtt ccggatccgc cggattcacc tcacgcgccg cctccagcgt ctgcaccgcc
     6061 tcgtcgaagc gcttgttctg cgtatagagc cgcgcgaggg cacggtagag gttaaggttg
     6121 ttcggctctt tctcaatccg gccccgtgcc tcctcgatca gggattcggc atcggtaccc
     6181 gccacgaccg ccttggcctg tatgtcgagt ttcttggcct gctccttgtt cgcgatcaga
     6241 ttccggtatc cgtccttctg tccggcaacc tcctcccatc cgccgctgac catggtcata
     6301 cgggcttccg catccttgag gagtttgaaa atcgtctggt cgttcggctt gtaggcgttc
     6361 agcttcagat acgcctcacg cgccttgtca taatcgccca ccgccatata gtaatcggcc
     6421 acgcgccgca gcagcgtcat gtcgtcgggg ttgttatcat aggctgcctc gaccgtgaag
     6481 agcgccgctt ccgggtgtcc ggcggcctcg gctacctcca ccgccagctc cacattctcc
     6541 gagtggagcg gattcatgat gagcagcttc tcgcactcga tcagcgccgc ctcatgcttg
     6601 ccggccttca tcagccccgt gaccttcagc cgctggaaaa aggccgccac ctcctgtatc
     6661 ttactggaaa gcggattctt cttggcttta cggaatttgg cgatctgcgc ggcccgcaga
     6721 aatttccggg cccgtgaaaa acccggcgcc aagatcaggc actgcatcag aaggtcaatc
     6781 gcgatatcaa ggttggcgcg ctcgaaggcg gcaacccctt tgttgtagaa gttctgggcc
     6841 tgctgggcca cctgctccgc tttctcattt tcacctatca tgccgcgtcc tcacttcttg
     6901 tcatacccct gctgggaatt agccttttca ctatacggcg aaaccttggc gccgtcaagt
     6961 tcgaccgccc gccggattgc gggcagcagc cgcaacgcca cctgctcatt gccggcgcgg
     7021 ttggggtggt cggttttgtc ccagtagggc ggaagccggt tgggcttgat gtcgatctgc
     7081 atgttgggca catactccgc cccatgcgtc tcgcacaccc ggcgctccaa ccgcgcgatc
     7141 tcgcgcgcca gcgcgaaccg gtccggcgtg aactccacgc acggctcctc ccagaaaaac
     7201 cggtctccga aacagctcgc caccaccacc tgcatcgagc gcgaacgcgc ttcgcgcacc
     7261 atcgcctcca aatccgcggc caacgcctcc ggcggacggt tgcgctgcca gaaatcgttc
     7321 gcgcccagcg cgatcaccag gatatcggga ttgtgcacca acacatcccg ctcgaaacgc
     7381 gcccttccgc ccatgagcgt gtctccgccc acaccagcgt caatcacccg gacatacagc
     7441 ataaaccgtg ccaaccaatc gctgtagcga ccgccctgcc cgccgcagga agtcagactg
     7501 tcccccaggc aaacgagcac cggccgggtg tcccgcaacc cccgaacccc taccgccgcc
     7561 gccagcccga acccgattgc caacgcgccc agccaaaccc atcgcctcat cgctccccca
     7621 ccgttttacc ctttccgttc cgcatggggt gtgttgtacc acatcacaca cgcggaagaa
     7681 agcccaaccg taatggatgc gggtccccga ggcaagggcg tgaggatgca accctgaaac
     7741 cagaaaccct gaaaccttga aaccctgaaa ccaaagaaag tgtcccgtgg ttaaacaatc
     7801 gattccgaaa gcgatagcga tcccgattcc gatttggatg aaaccgtgaa acacatgaaa
     7861 cttcatgaaa atcaggaatt tccccctggc gttccaaaaa aacccgagcg atgccatccc
     7921 gtgtgttcgt ggtgaaataa tcgattccga tagcgatagc gatcccgatt ccgatagcga
     7981 tcccgacccc gatagcgatc ccgatcccga tttggatgaa accatgaaac ctgaaacctg
     8041 aaaccctgaa accatgaaac catgaaaccc tgaaaccaaa aaaagtgtcc cgtggtgaaa
     8101 tgatcgattc cgattccgat agcgatcccg accccgattt ggatgaaacc atgaaaccct
     8161 gaaaccttga aaccctgaaa ccatgaaacc ttgaaaccct gaaacccaag aaagtgtccc
     8221 gtggtgaaac aatcgattcc gatagcgatc ccgaccccga tttggatgaa accttgaaac
     8281 cttgaaacca tgaaaccatg aaaccttgaa accctgaaac ccaagaaagt gtcccgtggt
     8341 gaaacaatat cggcggatgc ggcactaaaa accattgatc ccgtcgctct tgcttttacg
     8401 gcaatccgtc acgtatactg cggccatgtt tccttctgaa aaccagaccc aaccggatcc
     8461 cgcggcttgc cggccccgcg ccgccgccgc gcttcttcct tggctgactg tgccgcgttt
     8521 ctggtccctg accgcctcaa cggtcccggt caccgtcggc gcggcgctcg cggcccacga
     8581 cggccacttc aacgggccgc ttctggcgct ttgcctgctc agcggatggc tactgcaaat
     8641 cgcggccaac ctgctgaaca cttacggtga tttccggtcc ggcgtggaca cttccgcgca
     8701 tccgccgacc gccccgcatc tggtcaacgg cacgctgcgg ccatatcacg tcttgggggc
     8761 cggcgtgctt tgcctgcttg cgggggcctt ggccggcgca cgcgccgccg cgctgagcga
     8821 ctggcacctc gcgcttttcg cggtggcggg cgtggcgggt gccgcgtgct acacaaccgg
     8881 accgcgtttc aaatatgcgg ggttcggcct gccggtggtg gcgctgctga tgggcgtgct
     8941 gatggtcaca gccagttatt tcgcccaaac ccggaccctc accctcccga gtctgatcct
     9001 ctccctgccg gtcacctgtc tggtcggcgc gatcctgcac ggcaatgacc tgcgcgacat
     9061 ggcatccgac catcgggccg gcatccggac cgcctcgctg gttttgggtt ccgaacgcgc
     9121 gcccccgctt ttcatggccc tgcacatcct gccccacctg attctggtcg ccgggatcgc
     9181 gggcggtttg ttgccgcccc tgtccggcat cgccctcctc gcactgccgt tcaccctgaa
     9241 tgcgctccgc acctgcgcca tagggttccg cactggcgac cggacacgga tcggccgctt
     9301 ggaggggctc tccgcaggca tccactttct cttcggcacc ctgctcacgc tgggcctctt
     9361 tctcggataa aggaactctt catgcgcgca acccaccgga atttctggct actcacggct
     9421 ctggcggcca tcgggtttct ctgggtcttt ggctgtcctg caggcaactt ctggggcaag
     9481 atcaccctga ccgtgtgcgg actctgcacc gcctcaatcg tggtggcggg agtgccgtgg
     9541 cggcacaccg acctgacgct cgccgagggg ctcatcggcg tggcggccgc cgtggcgctc
     9601 tacggcctct tctgggccgg ttatgcgctg gcatgccgcc tgttcgactt cgcgccgcaa
     9661 caggtgaacg cgatctacgc gatccgcggc gcgggtcatc cgtggctgat cggcgctctc
     9721 ctgctgctgg tgaccagtcc cggcgaagag ttgttctggc gcggtttcgt gcaacgccag
     9781 gctgcgaagc gctggggcgc gacgaccggg gtcgttctcg gcacaacgtt ttacggtgcc
     9841 gtgcatctgg tttccggcaa cctcatgctg gtcttggccg cgagcgtggc cggactcttc
     9901 tggtgcgcac tctaccgctg gcgcggcaac ctcaccgctt gcatggtctc ccatgcgctc
     9961 tggaccgtcg ggatctttct gttcttcccg atccgttgac gcgcaaggtt tcgcgccgct
    10021 cctcctgagc cttctccgaa tgaaacgggg ctgcgcccgt atgcccgtga actcgcggtt
    10081 tgcgttcttg ccatcgggca gggaaacccg ttatgatacc gctcaaatgt gaacccctgc
    10141 ggttggggag tataggaggc agatatggcc ggtacagtat tggatttgac ggacgcgacc
    10201 tttgacggtg ctgtggcaca gggcgtcacg ctggtggatt tctgggcccc gtggtgcggc
    10261 ccctgcaaga tgcagacgcc gattctggaa actcaggtgg cccccgcatt ggcaggccgg
    10321 gcgaatgtcg cgaaggttaa tgtcgatgac gccaaagaac tggccgtgcg gttcagcatc
    10381 aagagcatcc cggccctgct gatcttcaaa gacggccagg tggtgcagca gatggtggga
    10441 ctgcaacgcg gcgacgcgct ggtgaaggct gtcgaggcgg tgctcggcgc ctaatcgccc
    10501 ggtccaatct ttccgcgatc cggctcccct gccgccagta tgcctgcgga cctccgctct
    10561 ccgggtccgc agcggcggcg gcgtgtattc ccatgaccga ttattccaaa ctgctgaacc
    10621 ccgaacagtg tgaggcggct atggcgggtg aaggccccct gctggtgctg gcggccgcag
    10681 gcacgggcaa aacccggact ttggtttacc gggtggccta cctcgtcgaa cagggccagc
    10741 cgccggacgg gctgttgctg ctgaccttca ccaaccgcgc cgcacgggag atgctggacc
    10801 gggccaaaga ggttgcgggc gagacggtcg ggaacctctg gagcggcacg ttccaccata
    10861 tctgcaaccg gtttctgcgg cgtttcggcg accgcctcgg gtatccccac gatttcacga
    10921 ttctcgaccg cgacgattcg cacagcctga tcgaccagtg catccgggag accgtgcgga
    10981 accgcaagga gttccccaag aaggacgtga tcgcctccct gatcggcaga gccgccaacc
    11041 gcgcctgcga tatcgaagat gtgctggatt cgacgctcac cgaactgcgg gtcgatccga
    11101 cggagatcct ccgcgtggcg aacctctacg cggagaagaa acgcggcatg ggggccatgg
    11161 atttcgatga cctgctggtg aacggactgc ggcttctgac cgaacacgag gcggtgcgcg
    11221 ttttttacca gagccgcttc cggcatgtgc tggtggacga atatcaggac accaatctgc
    11281 tgcaggcccg gatggtggac attctcgcgg acacgcacgg caacatcatg gcggtgggcg
    11341 acgatttcca gtgcatctac tcgtggcgcg gggcggattt ccggaacatc atggatttcc
    11401 ccaaacgctg gccgacctgc cgcatggtta aactggagcg caattaccgg agtgtgccgg
    11461 aagtgctgga ggtggccaac gcctgtatcc gcggcaaccc cgagcagttc cagaagaccc
    11521 tgcggcccac gcgcaaggcg cgcaacaaac cccaggttct gtttctacgc gacggcgagg
    11581 agcaggccgc ggccctgctc accctgatcg agcggtatca ggaggacgga taccggctgc
    11641 gggacatggc cgtgctctac cgcgcccact accattccat cgaattgcag atcgcgctgg
    11701 gacgcgcgcg catcccgtac gtgatcacct ccggcatcgg cgtgttcgaa caggcgcacg
    11761 tcaaagacgt gctggccttt ctgcgcgtct gcgtgaaccg ttccgaccgc ctggcgttcg
    11821 gacgcctgct gagtcttctg gacggcgtgg gcgagaaaac agtcgagtcc ctctggagca
    11881 agatcggcaa cacgtttgac acgcaatccc ccgaaacgcg cgcacgcttg cgggcggcga
    11941 tgcgtccggc ggtgcgcgaa cagtgggcgg cgatcgacca actgttccag gcttatcaca
    12001 gtgaaaaact gaataccgcg ggcggcgaga tcgtgcagcg cttttgcgac ctgttttacc
    12061 acaccctcct ctaccggcgt ttcgacaacg cggacaagcg tgaggaggac atccgcgaac
    12121 tggcgctgca gatcgggaaa agtccgagcg tggaggcctt tctgcaggag gtggccctgc
    12181 tcaccaatgt cgaccatgag tatgacgggg ccgaacgcga ggccaaagag cggttgcatc
    12241 tcagcaccgt gcaccaagcc aaagggctgg agtggcccgt ggtgttcgtg atctgggtca
    12301 ccgaggggat gttcccgtcc agccgctccg tgggcgaagc cgagaacgac gcggaggagc
    12361 gccgcctgtt ctacgtggcg gtcacgcgcg caaaggacga gctggtgttg tgtgcgcccg
    12421 gcatgcggcg cacacgggac ggcggcgtgt ttttctgcaa gccctcccgc ttcatcaaag
    12481 aaatcccgcg cggactgacg cgggagcgtt acggcaaccg gtacgtctga ggtcccgcgc
    12541 cgctcagagc gcaaggttga gactcacgcc gccccacagc agatcctcat ccctgtaggg
    12601 ttcggcatcg cgcgcgtcgc cgtccagcag cgacatccac gccagtttcg cgctgaccga
    12661 cagggtatcc gtgagcgcgt atatggcgta caccgagaag ttaaaatcca tcaggcccgg
    12721 ctcgtcaacc cccacatacg tcatatagtc gtcatccccc gcgccgaccg agacttcggc
    12781 gccgagtgcc agttggtcgc caagcgccac ctctttgttc aacccggcgt tgaagtaaaa
    12841 cccgtcaagc aggtcgtagt cataggccag actcgcaaac ggggtcacga gatcgttgtt
    12901 gtggctgacc gagagataga cctctttcgt gttcccgtaa gagtcgcggc cattcgcttt
    12961 gaggaaggtg tacccgaaat gcccccactc cagcgtgaag ccgcccacat caaacgcgtg
    13021 gaacagcgaa taatcgactt cattgaaact gccgcccgag accttccggt tgcggtcggt
    13081 cagatcaaga ttcccccaga ggcctaggcc gaacgtcccg taccgctctg tcgcatacgc
    13141 cagtgtcgcg gcaggctgga agaccggctc gtcgttgtat acgcagccac gccagacata
    13201 cgcggagaaa agatccagcg tcagaccgaa cgagaaccct gccgtctccg cctctcccac
    13261 cgctaccggc gcggcctcct gtgacaacgc gccgcctgcg agaccgatcc acaacgccgc
    13321 cgccaacacc ccgtttctca tgtttttctc tttcctaacc ttattgcgac ctcaccgctt
    13381 tccatcgggg gaagaaggca ggaccgttac accgggcagc cgttgctcgg cggaaaccca
    13441 tccgttggtc cacgctgtgc cgggatcgaa acgcgcgggg tcagggaaaa gatcgtccgg
    13501 ggtatgcggg atccggtcct ccccgcagga aaagagcgtc ggcaacccgt tcccggccgg
    13561 aggcgcgtaa acgaagggcg tgttccaagg gtcgttgcgc agaaaacgga tgtagggccc
    13621 gtcccacttt gggacagcgg gatcgcgcac cagcgcggca agccccggca cagcgtcggg
    13681 gaagcttccc gtatggaagt gatagcggcc cagagcctcg gccagcacat caaggttgcg
    13741 gatcgcgcgc atgtgcgggg tcacctccat ccgccgcacc gccttgtccg tggtgttgga
    13801 cagcaagatg cccagaaaca cgaacaccac gatcgccgcg aaataaaact tggggttgcg
    13861 gaaaagggtt ggcgacaccg gattgccgag cgcggcccgc ttctgctcgt actcgcgcat
    13921 gatacggctg atcgcgcggc gtttgaggcg ccagcgcacg ggatcggagc ggggcggcac
    13981 gcgcatgact ttgtcgcacg ccggacaacg cgacgcgggg cgctcatcaa agttatgacc
    14041 gcaatatggg caacggtagc gcataggaac gaaagtaaga ggtgataaga ggcagcaggc
    14101 agtgggcagt gggcagtggg cagtgggcag tgggcagtgg gcaacaggtg ataagcggga
    14161 gcagaaactc ccaactccca actcctaact cctaactcct aaatcctaaa tcctaactcc
    14221 taaatcttcc ttccctttat gggtccagat ttgtgagtgc aagcgtgtcg agatcgatac
    14281 ggcggtagta gcagttgcgc ggctcgccgt tccggttgcg ggtgtggcag atcgcgccgc
    14341 gccgcggccg cacaatgtac aggagcgagt tctgctcgca gttgacgcgc gcctcgagca
    14401 gatcaaacgt ctcgcccgaa gtcctgcctt tttcccacag cgtgttgcgc gaggtgctcc
    14461 acaacacgag tgtgcgttcg gcaaaggcct tacgcatcgc cagttcgttg gtgtaggcca
    14521 ccaggatcac ctcccgggtg tccgcgtgct gcaccgccac cggaatcgcg tctttgcagg
    14581 tttcggcgat tttcgcgatt ttcgcgaaat ccagcgtcag ggttgcgcct tcttccagtt
    14641 cttggctcat ggtgttcctt tcattcgcgc gtcagtaggt caattggctg ccgccgatgt
    14701 actcacgcaa aatcgaatcc cccggcacga catccggcga cagcggcgtg aagttgtgga
    14761 ggaatccgga gaggcggcgc gcctcgatat acacctcgtc ccaaggtttg atctggttga
    14821 gcagcggcgc gatcaggggc ttcagcacag ccagctcata gtccagcgcc gagttgaaca
    14881 ccgcatcagc gagatgctca aacgggtaga tccagcgctg ttcgccgcgc acgatcgagg
    14941 gccacatgcg caacgtctcg atggccggac ggttgcggaa ccggtggtcc cgcaccatgc
    15001 gccggatgat ccgcgtgtcg gtggtggaaa tgcggttgct gctgtccacg ccgagttgcg
    15061 tgagcgcgtt gatgtagatc cggaatgtcc gatcgcgggg cacgtccgca atcaactggg
    15121 gattgaggct gtgaatcccc tccatcacga tgatgccgtt gtccggcagg cgtgtggtct
    15181 ccgggcggtc gtacccctcc ttggtcttga aatcgaagcc gcgcaagtgc acctcctccc
    15241 gcgccagcag gcgcaacaga tcgcggttca ggcgctgcag gtccatggcc tcgatatgct
    15301 cgtagtccag ccgtccctcc gcatcacggg ggttgcgcgc atctcccacg aaatagttgt
    15361 ccgtggagat cagcagcggc cggtagccgt tcacgcgcag gtgcgtgatg agccgcttgg
    15421 aaaaggtggt cttcccggcg gaggagggtc ccgccaccag caccagccgc accgaaggct
    15481 cgcgctgcgt aatctcatcc gcaatgcggc cgagtttttt atcgtgcaac gcctccacgg
    15541 tctggatgaa gtcatcgacc cgtttctcga tcaccgcctg gttgagttcg ccgaccgtgg
    15601 tgatgcccac gatccggccc cactcgatgt gctcccgata cacccggaag aggtgctcga
    15661 acggcggcag cggcggcagc gcgctcggtg aatccggcgt cggcaccgtg agcacgaacc
    15721 cgcaggaaag gggagtgagg tcgaacaggt tcagcacgcc ggtgcgggag acgagcggct
    15781 cctggctcaa ctcgaaaaag gtgccgcagc gcgttaggca aatgaccggc ggattccggt
    15841 gcgccagcag gttgagtttg tcggtctgtc cggtttcgcg aaaccgtttc accgccgtct
    15901 cgtacgacac cgtttccgtg ctgatcgtga gatcctgccg caccgtttcg agcatggcct
    15961 gtttcagacg tgccacctga tcacgcaacg cgtcttccga acattcctcc cactcgaccg
    16021 tgcagtagag acccgtgccg atcgagttgc gcacgcggca gcccacgtcc ggaaaaagct
    16081 cgtggacggt tttggccaac aggaaacaga gtgacgcgcg gtagatggcc caaccgcggg
    16141 gatcccggat cgtcaggggc tcgaccttcg catccgtgat gaacgacata aaaagaggct
    16201 gcaccatgtt gttcactacc gcccccagca cggggaaccc gtcccggtcg accgaaggca
    16261 gcaattcgct gatacgagtg ccgacggcca cgaccatcgg ctcctgccct ttgactgtca
    16321 cggtaatggt gtgcatgacg cttcctgttc cgggttgtta ccctttttcg cctccttcag
    16381 cgtaccctca aacaggtcaa agaccagcat gcggcactcg atcggcccgt tgaaaaaagg
    16441 gatgcgccgc gacgaacgca gcccgatacg gcgcgacaac tcgaggttac ccgtcaggac
    16501 gcaaccggta tagcccacgg cctgacgctt gaaaaaatcg ccgatgcggc ggtaaagcgg
    16561 ctccagcgcc tcacctgacg caggctcatc cgcagcagac tcggcatccg tttccgccgt
    16621 gctctccact gcgacgcccg aggccagacg ctgcccatag ggcgggttca ggaaaatcac
    16681 ccccggcggc gggggcaact gcgtcgcctc gaaatcgcag gcggcaaagg tgatgtgttc
    16741 ggagacgccc gccgcgatgg cgttgagccg cgcggattca agcgtctcgg ggtcgatgtc
    16801 cgtggcgagg atcggcggca ggccctcccg gcgttcctga tcgcgcgcgg tagccaccat
    16861 gtctttccaa atctgttccg gcgaggcacc gaaacgttgc cgcacactcg ctcccgcgcg
    16921 ctcgccggga atcgtgatgc ggtacccttt gatggccatg aacccgaagt ggctcttgaa
    16981 gctgcccggc gcacggttga gcgccagcag cgcggcctcg atcgccgggg tcccgctacc
    17041 gcacatcggc acgacaaacg ggctgttggc atcccatccc gaagccagca cgcatccggc
    17101 cgccagggtc tcctgcaacg gcgcggttcc cggcgtgatg cgatacccgc gtttggagag
    17161 cggctcgcct cccgtggtgt ccagatagat gatgagctcg gttctctgcc aatagacaaa
    17221 gattgcggcg ccgtgggttt cgcggcccga atcgggacga ctgccgcact gctgccgcat
    17281 acggcggcga tcgcgtcttt ggccttgagc gcggccatgc gggtgtcgcg gatcgcgtcc
    17341 gtgcgcgtca cgctgttcac cgtgaaatac ccgtccggct ccagcaggtt ttcccaatcg
    17401 atggatttga cgtcgtcgta aaactcgcgg aggttgcggc actcggtccg caacagcggc
    17461 gcgagcacac ggtgcgcggt gcggagccgc aagttcagcg tcatcatgtc gcgcatggtg
    17521 ccgcggacca ccaccgtctg tgcggtggtg tccacaatgc ggtaaccgag ccgcttgagt
    17581 tcgccgaccg tctgcggcgc caacccgcgc gcgcaggaga tgatcagcgg atacgtgtct
    17641 ttccaaagat tcatgttcag gccttacaaa accacaaagg tcacaaagat tccggattcc
    17701 gacctcttcg tggccttcgt ggctttgcgc aaacattccg accgggcgga tgccccgtca
    17761 gaattcccag ccgggacgga tgtgcggttt gaccaacgcg ttggctgcgg cgttgttggc
    17821 aaactcctgt ttacggctgt cccacttcag cttgccgggg acctgctgcg cgacgcagcc
    17881 gaccagcatg ctttccagca tcggcaccga atggtccaca tccgagaagc acacgtcctg
    17941 acccttgcag gcgttgacga actcgaggta gtggccattg gtgttggggc ggaacttcgc
    18001 cgggatgact gcctcggtca gcgcctcatg cttggacatc gacttcatct tcgcctcgtc
    18061 gttgaaggcg atgtagccgt ccgcgccgta gtcgttggtg ctgcagacca tgcctttgct
    18121 gcccatgatc aggcagccgg tgttcggcac ctgacccagt gtcgcgacga ctttgggcat
    18181 gatctctgcc gaaggcttga gattgccgtc ataccagtag agcgtcaccc gcggcagacg
    18241 gccgcgcgcg gcataggtca gccgcacgat gctcttgttg ggataggtct cgttattctt
    18301 accctcgatc tggatgcact cggccgactc gaccgcgccc aggcgcaaac cgcggaacgg
    18361 caggttcatg gtgtggcagg ccatgtcccc gaacgcgccg gtgccgaaat caaaaaagcc
    18421 gcgccacttg aaggtgtggt agacgccggc cttgaacggg cgcaccggtg ccgtgccgag
    18481 gtagctgtcc caatcgagcg tctccggaat cgggtccgac ccctcgggcc tgccgatgcc
    18541 ctgcggccat atcgggcgat tggtccacac atgcacctcg gtcacatcac cgaggatacc
    18601 ggcttgcaac agttcagcgt tacggcgcag gccgttgttg gcactgccct gattgcccat
    18661 ctgcgtcacc acaccgcagc tcttggcgac cttttcaaaa taacgcgcct cccagatcgt
    18721 gcgcaccagc ggcttctgca caaacacatg gcacccctgc ttcatggcgc ggatcgcggc
    18781 gggcgcatgc atgtggtccg gcgtcgaaac cgtcacggcg tccagattct tgcactcgtc
    18841 cagcatctta cggaaatcgc tgtagcactt gacctgcgag ggtttgaccc ccgctttctc
    18901 caactcggcc aaggccttgt tgatcggatt gcggtccacg tcgcagagcg ccacaatgtt
    18961 ctcgccgttc ttgaacatcg gcatccagtc gctccatccc ttgccgccca caccgaccac
    19021 gccgatattg agcttctcgc ccggcttacg gaatctcggc gcgaccgtgc gcgtcaccgc
    19081 cggatagcgg cccgccgctc cggtggtctc gcatccgccc gcgaccagcg acgccgccgc
    19141 cagtgccgat cccctgataa aatcacgacg attgaactgc atcatgttga tccccttttc
    19201 ctttctgtaa tccatccacc ttcttcatac aaacaaaaca ggaaaagagt ttaattcgtt
    19261 tcgtgccgaa aaagcaactc tttgctacga ttatttcaaa tcggaggaag aggatgaatc
    19321 aaaaagaccg tgctttttta gcggcccgcg tgggccggat ggaacaaatg gccgcgattc
    19381 ggcggttggt ggaggaggac ggccggggac gcggcatgcg cgtactggac ttcgaatccg
    19441 gcagcggact cgcgttttcc gtgtatccgg accgcggcat ggatatcggc ccggcgcggt
    19501 acaaaggcat cccgctggcc tggctcgcat gccagccgcc cgccgtgccc cacgcctacg
    19561 accccgaggg atgcgagtgg ctgcggacct ggggcggcgg cctgctcacc ggttgcggcc
    19621 tgctgaatgt cggcggaccg tgcgcagcgg acggcgaacg ccacggtctg cacggccggt
    19681 tgagccacct cgcagccgag gaggtgaaca cctccgcagc ctggacgcca gacggcacgt
    19741 ataccctgag cgccagcgga cgcatgcgtc acgcccgggt cttcggcgag aacctcacgc
    19801 tcacgcgcac gatctcgacc gccttcgaca cgccgtccat caccgtgcgc gacacgatcg
    19861 agaacctggg cttcgcgccc acgcccgtga tgctgctcta ccacatgaac ctcggctggc
    19921 cgctggtgga cccggaagcc acacttgtga tgcccgacca tcccgtgacc ccgcagaacg
    19981 agcatgccgc ggccgggctc gcggagtggc agcgtttcgc cgctcccgcg ccgggcttcc
    20041 gcgaacaggt ctactaccac accctgccag ccgacgaaca ggggctggcc tcggccgcgt
    20101 tggtgaatcc gcagttgcgc ctctccctgc gcgtgaccta ccgcgtggca gaactccctt
    20161 tcctgattca gtggaaaatg tgcggccaag gcgaatacgt tctggggctt gaacccgcca
    20221 actgttaccc cgagggccgg gaggccgcag cccaacgcga tctcttgcgc acacttgaac
    20281 cgggtgaacg tctggaaacg ttcctgcgcc tctcggtcga accgctcgcc taagccccat
    20341 gcccctgccg tgcacacagc gggagatccg gcaactcggc tgggacgcgc cggatgtgat
    20401 tctggtctcg ggtgacgcct acatcgattc gccctattcc ggcgtggccg tgatcggtcg
    20461 tgtgctcgaa cgcgaaggct tccgcaccgc gatcctgtcc cagcccgatc ccgcctcgac
    20521 ggaggccttc cgcagcctcg gcgaaccgcg cctcttctgg ggcgtcacca gcggatgcgt
    20581 ggattccatg gtcgccaact acaccgccgc cggccgccgc cgcagacagg atgacttcac
    20641 tcccggcggc gtcaacaacg cccggcccga ccgcgcggtg atcgtctact gcaaccaaat
    20701 ccgcgccgcc ttcaagccct gcaaaccgat cgtcatcggc ggcatcgagg ccagcctgcg
    20761 acggatcgcg cattacgact gttggtccga taccgtgcgg cgccccctgc tgttcgacgc
    20821 caaagctgat gcgctcgtct acggtatggg ggagcgcaca atcacggcgt tggcccgcgc
    20881 atggcgtgac ggtaccgact ggcgcggctt gcgcggcctc tgccacgccc tgccggcctc
    20941 atcgcaggca cggcccgcaa acgctgtcgc tcttccggat ttcaacaccg tggccgcgcc
    21001 caccccggaa gggcgatccg cctttctgga gatgttccgc ctcttcgcaa ccaaccaaga
    21061 ggctcacacg gccagtcccc tcctgcaaaa agtggacacc cggtgcctgg tgcacaaccc
    21121 gcctgcgccg cccctcacct cggctgagct ggatgccgtc tacgccctgc cgttcacgct
    21181 cgacccccat cccgctgtgg cggcccgcgg ccccatccgc gcactggaca ccatccgttt
    21241 ctcgctgacc acccaccgcg gctgttacgg ggcgtgccgc ttctgcgcca tcgccatgca
    21301 ccagggtcgc caagtggtca gccgctcaca atcggcgatc ctcgacgagg cccgccgcat
    21361 gacacgccat ccggcgttca ccggcatcct ccgcgacgtg ggcggaccca ccgccaacat
    21421 gtacggcttc gactgttcgc gcaaaacgct ccaaggcgcc tgccccgacc gggaatgcct
    21481 cttccccgcg tgctgtgact cgctgcgccc caaccacgga ccgcagcggg aactgctcgc
    21541 cagcctacgc cgcctgcccg gcgtgcgcaa ggtgttcgtg gcctccggca tccggccgga
    21601 cctgattgcc gcagacccgc gtcacggcga cggctacatc gatgatctcg cggcccatca
    21661 tgtctcggga cagatcaaac tcgcgcccga gcacaccgaa ccgcgggtgc tcgccgccat
    21721 gggcaaaccc ggtctcgacg cgcttctgac cttcaaacgg aaattcgagg ccgccaatcg
    21781 caggcacggc ctccggcaat tcctcactta ctacttcatc gctgcgcacc ctggctgcac
    21841 cgatgacgac atgcgccgcc tgcggcggtt tgtcgcctcg catttgcacc tcaaccccga
    21901 gcaggtgcag atcttcaccc cgacgccctc tacttggtct accgcgatgt actacaccgg
    21961 ttacgacccg ttcagcggca cccctatcca cgtcacccgg aacccgcgcg acaaacagcg
    22021 gcaaaaggcg attctggcac ccgacgcggc gagagcccgt aacctcgtca tcacgaaacg
    22081 gggataatac aagacgtaac ctaaaaagag cagttgaacc ttcaactctc cttggtttca
    22141 aggtttcggg gtttctggtt tcagggtttc aaggtttctg gtttcagggt ttcatccaaa
    22201 tcgggatcgg ggtcgggatc gctttcggaa tcgattgttt aaccacggga cactctcctt
    22261 ggtttcaagg tttctgggtt tcagggtttc tgggtttcag ggtttcatcc aaatcgggat
    22321 cggggtcggg atcgctttcg gaatcgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22441 nnnnnnngtt tcaaggtttc tgggtttcag ggtttctggg tttcagggtt tcatccaaat
    22501 cgctatcggg atcgggatcg ctatcgggat cgcttgctta accacagcct cacagcctca
    22561 cagtctcaca gtctcacagc ctttctcctc gcttacacaa gggattccgg cggtgccaga
    22621 cggcaaagca gcgcctgcgc ggcagccgtc tccgcggccc gtttggtgcg gccctctcct
    22681 tctgcctcgt gccctcccgc cacccgtacc cgcacggtgt agaccggccc gtggtccgga
    22741 ccgctcctcc cgatcagttc ataggtcggc gaattcggcc aggcgtgccg ctgcgccaat
    22801 tcctgcaacg cccctttagg attgccacgc caaggcacgg cctcctcttc ctccgggtcc
    22861 agccgccggt agacgcgcca caccgcgtcc catccgccct cgcgccatat agcgccgaaa
    22921 accgcttcca gcgcgtcggc gagcatacgg tcctgatcgc gcgcgccatt ctcctcttcg
    22981 cgctgaccga tccgtagaca ggcaccgagc ccgagccgac gcgccaagtt ggcgagcgcc
    23041 ccgcggttca cctgcagact gcgccgcacc gtgaggtttc cctcatcctc ggccagataa
    23101 cggtcgtaga gactctgcgc ggcgagcagg ccgagaaccg catcccccag aaattccagt
    23161 cgttggttat ccggaatcgc gcccaccaca cgccccgcac ggaaggaggg agaggtcaac
    23221 gcggtgcgca gcagttcccg gtcgcggaag acgtatccca accgcgcctc gaacgcatcg
    23281 agcggatctg tctttccttc cggcaccggt cgcccctccc cttttcccgc gacggtcaat
    23341 attcgatctg gaaatcgatg aactcggccg ggatgccgag ccgccgccgg atgagttccg
    23401 ccaacgcccg gacaccgaac gtctcggtgg cataatgccc ggcgaacagc acggtctgcc
    23461 ccaactgttc ggccaggtta tacccctgca gactggactc ccccgtaaga taggcatcga
    23521 ccccatgtgc ggcagcctga tcgatcatgt ccgccgcccc gcccgaaaca acgcctaccg
    23581 tccggatact acgcttgccg aagggcaaca ggcggcaggc cggcgccaca accttctgca
    23641 cccgcttgaa aaacctttcc aatggcattg cccgctcgta ggcggcaacg aagccgatgg
    23701 tctccccgtg ataggtaaag gccggaaccg gccgccgcaa cccgagggct ttgcagagcc
    23761 gcgcgttgtt accgtagcgc ggatgggcgt ccagcggcag gtgcgccgca tagaccgcga
    23821 ggttgtgcgc gagcgcgaag gagaccagcc ggtggttcag cccggtgatc cgcttgagcg
    23881 agtcgcccca gctcacgccg tgatgacaaa ccaccgcgtc cgccccccgg gccgccgcgg
    23941 cctccaacag gcgcagactg gcgtccaccc cggcgaccac gcgcgtcacg cttccgttgt
    24001 tagcgatttg caacccgttg ttcgacgcgt cacgaaaacg gccgatctcc agggtctcgt
    24061 ccaacaggct ctccaacgcc gatagggtaa ccgtcatcat tttatcccgg acagcgattc
    24121 ctgcactgcc ccatgtgatt tataatgtaa tcgttttacc tgtggaacgc aagggaacgg
    24181 tatcgggtcg tgacaggggg gggagtcatc gtgtacgggt acgtgaacga ggacgggtat
    24241 ggggcatggc caacgttcac gttctccaaa tcgctatcgg tatcgctatc g
//
LOCUS       DAOSLE010000020        24243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2310_length_24243_cov_7.702828, whole genome shotgun sequence.
ACCESSION   DAOSLE010000020 DAOSLE010000000
VERSION     DAOSLE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24243
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2310_length_24243_cov_7.702828"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK576_02780"
     CDS             <1..638
                     /locus_tag="PK576_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOE60545.1"
                     /translation="VIREYGADSMRLYEMFMGPLEAVKPWNTKGVEGVFRFLKRAYRM
                     VTEQPITEDPLQPEQLRMLHATVKKVTDDLEDMGFNTAISQLMIFLNAFSGADKPLPR
                     LAAETFVLLLSPFAPHLGEELWQHLGHENSLAYAPWPVYDESLLQLDEVEILVQITGK
                     PKARVMMPADADEETMREMALSQSVIREALTGKTVRKVICVPGRLVNIVAT"
     gene            830..1528
                     /locus_tag="PK576_02785"
     CDS             830..1528
                     /locus_tag="PK576_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOE60546.1"
                     /translation="MIPYFYDPRLILVMLPALILAGIASVLVKTTFNRYARVRAYSGM
                     TGAQAAERMLRANGVFDVTVEETGGFLSDHYDPTRKVLRLSPDVYRGNSLSAVGVACH
                     EAGHALQHANSYGPLMLRTALVPVTSISSSLAFYVVAAGFLFRPLLLVGCAMFAVAFL
                     FSLVTLPVEWNASARAKQHLVTSGIVSPGQEAEAGRVLNAAFLTYLAAAVSSLITLLY
                     YLARAGVIGGGRRD"
     gene            1551..3431
                     /gene="htpG"
                     /locus_tag="PK576_02790"
     CDS             1551..3431
                     /gene="htpG"
                     /locus_tag="PK576_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOE60547.1"
                     /translation="MSKKTHVFKAEIQQVLDLVIHSLYSKKEIFLRELISNASDAIDR
                     AQYLGLTDPTLIADAPAWRIDILANREERSLTIADNGIGMSEEELDKNLGVIASSGTK
                     AFIKALREKESVDIPELIGQFGVGFYASFMVAEQVTVITLKRGADQKAVKWSSTGDGG
                     YTLEDAERDQPGTTVVLKLREDMKEYLDEWRIRELVKTYSNFIAYPIRFSDKGEMPAE
                     DSEPLNTIKALWRRPKSEIKEEEYNEFYTHLSHDHRPPLKTIHFAVEGVTEFRALLFL
                     PKEAGMDLLLPHKQRGLSLYVRNVFIGSDFDLLLPDYLRFVKGVVDSSDLPLNVSREM
                     LQDDALIRKIKSNVTGKILATLTEMMRDEPEAYSTFYTSFGRILKEGLHWDWENSEKL
                     KSLLMFRSAKQENNGFLSLKAYVESMPESQKDIYYLLSDDLATARQSPHIEALLQHGY
                     DVLFFTDAIDQWVVERLREFDGKKLVAVDKGTLELGTEAEKAETKKRIETADAEFREL
                     SAFLKEHLKEKVGEVKLSTRLTDSACCLVADEHALNASMERLMRAMNQEVPKQLRTLE
                     INPDHPVLKRMRQMLETDKSDPKLADYADLLLGQALLSEGSALPDTQRFTRLVSELMS
                     AS"
     gene            complement(3415..5229)
                     /locus_tag="PK576_02795"
     CDS             complement(3415..5229)
                     /locus_tag="PK576_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOE60548.1"
                     /translation="MKNRCDAADGEEGQQVFCHLWRLRDPGGGSWSGELAESALATAL
                     AAAALADAGEADDRVQADSGLNWLACHVNPDGGWGDTPHSRSNLSTTLLVHAALQRFA
                     GERHAKTVARAGEWIDRQLGEARSPASVARALRRTYGEDRTFAVPIQAFLAMCQDTRQ
                     AWQWVMPLPFALACLPQRLYRLLRLQVVSYALPALIAVGLCRHLRVARNRRIAAWGRV
                     LAPALLRKLAALQPDHGGYLDAIPLTAFVVLALHHAGFGGHAVVGKGLAFLRHSARAG
                     GGWAIDSNLRTWVTSLAARAITAYREGAGAGRVEAEALTPIARWLIQVQKTERHPFTG
                     AAPGGWAWTDLPGGVPDADDTAGALVALKRLRDAGCAVDFRAAVRDGVRWLLDLQNRD
                     GGLPTFCRGWGRLPFDRSCPDISAHALAAWAAWRNDPALPSYAERKVDSAIRRVLGYL
                     KQEQQPDGSWIPLWFGHQDAEHGQNPVVGTARVTDALRAAHYAADAVGWAGEAEAMRT
                     AGERWLIRSQKPDGGWSAGKTATVEETALAVIALLGGDAPCRAAAERGRNRLKEAWRS
                     GNVPQATPIGLYFSVLWYHEQLYPIVSSLEAFAVRTPT"
     gene            5472..6920
                     /gene="rpoN"
                     /locus_tag="PK576_02800"
     CDS             5472..6920
                     /gene="rpoN"
                     /locus_tag="PK576_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE60549.1"
                     /translation="MAVPSLSLSQQQRQVMTLAPQLRQSLEMLQMPVMELRAAILNEM
                     SVNPTIEEVEDPSEVSLATVTAPEPQQQSAADEELDFNPDIDAILRQDDEWRDYFMQG
                     MENAPSYEEENEKRQYLLDSIRQKASLQDYLLEQLTLTDLQGEDYDLAVLLIGNISDD
                     GYFTGSLPDIIMVSGRSEREVLDVLKVIQTFDPPGVGARTLRECLLLQLALVEDSPWE
                     DEARLVIDKHLERLGAHDEKYLCKVLELTREELAHVTALIRSLNPRPGRAYVQEKTEY
                     VEPEVFLVREKGRLVARVDNQRLPHIHISKHYRSLLEDRNTPAETKTYIRERIRAGAF
                     LIRSLYQRQETIRKIAQEIVDAQADFWEKGVMALRPLTMAEVARKVGVHETTVSRTVS
                     NKYMRTPRGVFEMKYFFTHGLKSEDGVSVSNKSVQDQIRRLVEAEDPTAPLSDQALEE
                     KLSEQGIKVARRTIAKYRGILKIPPSHKRRRT"
     assembly_gap    6948..7047
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7132..8142)
                     /locus_tag="PK576_02805"
     CDS             complement(7132..8142)
                     /locus_tag="PK576_02805"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE60550.1"
                     /translation="MAFGEILKNARLQRGLTPSDVAESTHLLVQVVEDLEREQFSRVA
                     APIYGRGFIKLYAELLELDPEPLIREFMDLYTGARVPAIRTRDVPHEAEQDAAETASA
                     VTPRQAPEAGPQRQRIEPRPAVMVRPVKEAPESAAPPAEDADRVAVKSRTEETGAERE
                     VRSARHGAHDLFGWVVRPEEEEKPERDVPQEPDLFHPAPSVTPRQPDAADAEKRKSAD
                     RFAVAQRRVLPIFKIGGRANDRAPGGFADEDSDAHARRNARIRAFKEGFMKLRTDVGR
                     RLPVTLPHKSVLMLAGAGVVVLICLLAGISLLFRLTGSGVSEGPAPVFETVSPPPDLY
                     ID"
     gene            complement(8161..10656)
                     /locus_tag="PK576_02810"
     CDS             complement(8161..10656)
                     /locus_tag="PK576_02810"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOE60551.1"
                     /translation="MSERLIEQQDRMELERRIQRRWTGVLLFPIALFPLLSLLTYNWR
                     DISWLQAPPLTPPSNLIGIVGAWSVFIGYSLVGLALWLVPVMVLLFSVMLIYGRAVRL
                     VRRAFWMLIFLIALCCLLQLGSATVFDPVLERLNLKPNAGGAVGYVLMTCLLARWFSP
                     VGGGILMLSIMLCSLFLAVGLRNIVAGLGWVTGWWTTRPRAADKTEPPGTAEKEDSEA
                     DDGLTRRERERQQRQAIREAARRAKEEARQAREQERQREPAPAAPAPAPRVEPQQVVP
                     EKAAGTRPAPAVVAQPEPAATLPAAYELPSVRLLNPIPKGAADHGNITETSDILVETL
                     SQFNVPVEVVGTTPGPVVTQYELRPAPNIKVERIANLTANLQMALEATSLRVQAPIPG
                     KNAVGIEIPNRRARAITFREIIEGEAWTRNTCEVPLLLGKDVAGDDVVFDLTKAPHLL
                     VAGATGSGKSVCLNAILTGFLMSRTPDQLRLILVDPKRVEFTCYNELPHLLVPVINDP
                     KKVAFGLRWAIMEMEKRYRLLQKEGCRNIVAYNSRVIARQDDLFGEEDALSPQASAVD
                     ETPDRLPYIVIVVDEVADIMASVGKEVEPAIARLTSLSRAVGIHLILATQRPSVDVIT
                     GTIKSNIPGRIAFKVSQSNDSRTILDSPGAEELIGKGDMLFLREGGQLTRLQGAWVSD
                     EEIARVVAYVKEQSAPCYDAALSGRLEKIRESEPGAALSDEEDGEGGSEQGMEDGESD
                     DVLLPNAIEVIRMTRRASTTMLQRRLRIGYTRAARLIDILEERGVVGPHAGSNSREIL
                     IDLDKEIPVGGGTDRAGAPLPDEEAEADESPRV"
     gene            complement(10662..11675)
                     /locus_tag="PK576_02815"
     CDS             complement(10662..11675)
                     /locus_tag="PK576_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60552.1"
                     /translation="MQRDKVLSILRIVFLNNGYWKLLSLVIAVLVYFSIRAEISHVRV
                     ISIPVEAEFDAAGAGAAIESVEPRSVQVTLRGSYTDVNQLSLSVARCVVRPRQKKGNL
                     VDSVPVKIRRGNLRGVRGIRVVKVDPNVAVVKFDVPMSLPLMIARPAEIGTARGRVQL
                     VYDLTNAVVRGSRRQLSTLNPEVTQIQTEPINVDGRSQTFSTRVRLLPPGDVVNATVE
                     PPEMVVQVQILSEKATAKLERVPVVVYPHDMVQRWHVEPECVEVELSGRGEIVKAVTP
                     EQVTAVVSGNLPLSRETNGVPVMIHLQQGLEVSEVRALPPTVRLIPVTASVVGADTPA
                     GQK"
     gene            complement(11662..12525)
                     /gene="cdaA"
                     /locus_tag="PK576_02820"
     CDS             complement(11662..12525)
                     /gene="cdaA"
                     /locus_tag="PK576_02820"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOE60553.1"
                     /translation="MFDGVQIGFNDVMQILILYLGIYALIRFVKGTRSAQVLLGFCVL
                     VVSLLLFTYLFRFDVLAWVVYFFLIYLALSMVVVFQQEIRRVLALVGGQRLFSRQYTR
                     QQSSVPELICQSVMNLSRARIGALIAVERGISLCGYEETGVKLNALFSHELMVSLFTP
                     PLPLHDGGIILRDGRVASAHCLFPVSNQGELAASGMRHRAAVGLSEETDAVVIVVSEE
                     TGRISAAYNGRLHQYEDDKIGKMILRWLRVAMPQQTGDASTVTEWLTLKLEKWLKPLA
                     MSVGGDDHAKG"
     gene            complement(12518..13387)
                     /gene="folP"
                     /locus_tag="PK576_02825"
     CDS             complement(12518..13387)
                     /gene="folP"
                     /locus_tag="PK576_02825"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOE60554.1"
                     /translation="MERDSGRTWVCRGCVVPLRHTLVMGIVNVTPDSFSDGGKWFDEE
                     RAVAHALDLVEEGADILDIGGESTRPGAEEVSTEEETRRVAPVIRMLAERSGVPVTVD
                     TRHAAVARAALEAGACAVNAVQPFTEDAEMVRLVRDSGAGVVLMHMRGTPRTMSDLTE
                     YRDVVGEVEAMLRDALACAVESGIGRERILLDPGIGFAKDTAHSVALLAATARLSRLG
                     PLLVGASRKRFVGEVCGEPDARERVGGSVGAAVWCALHGAAVVRVHDVKATRQALAMV
                     RRLAEATVGETHV"
     gene            complement(13417..15450)
                     /gene="ftsH"
                     /locus_tag="PK576_02830"
     CDS             complement(13417..15450)
                     /gene="ftsH"
                     /locus_tag="PK576_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE60555.1"
                     /translation="MSKESDHKRELPNQPPALPNRGGKPEGGATSGALPPKRVFGAWM
                     VVGLLLLFMLGMFYYKPEGAMRPMDQLEFRGLVEEGLIEKVELVREANGMTYLRGVRK
                     GAAKERFRVNLIPSENLEQFLLEKKIPCPLVYNTNVFGALLRDILPVILILVILYFVF
                     IRQIKSANMGAMSFGKSRAKMLSQDKKKLTFANVAGIREAKEEVQEIVEFLKDPRKFQ
                     VLGGRIPKGVLLMGPPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDM
                     FEQGKKHAPCLIFIDEIDAVGRSRFSGIGGGHDEREQTLNALLVEMDGFETSEGVIII
                     AATNRADVLDPALLRPGRFDRQIIIDLPTLKGREEILRMHAEKIKLAHDADLSRIARG
                     TPGFSGADLANLLNEAALLAARANKRAVGQSDLEEARDKVLWGRERRSRAMDDRERRI
                     TAWHESGHALLQVLCEHAEPLHKVTIIPRGQALGATMSLPERDVLNRTRSELLDQLVV
                     LMGGRIAEKFFTADLSTGARMDIKMASEIARKMICSYGMSDRFGFQSFGDNQETLFLG
                     REVARNQAYSEETAKEIDAEVSRLVTESYARAERMLQDNRAKLERLVECLLEAETLDG
                     RDVESLIRQGRLPDEVSSLEDGQADEPASTDPAVPVDKETPQDKPDEPPEFLL"
     gene            complement(15526..17118)
                     /locus_tag="PK576_02835"
     CDS             complement(15526..17118)
                     /locus_tag="PK576_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOE60556.1"
                     /translation="MLFGWFKKGAPKREIVVNVEKLETRVAVVENGRVEEFQVEHPTE
                     ERLVGSIYKGRIQNLEHDLQAAFVDIGMKKNAFLHYWDMLPDDDSRLDDDEENVRPSR
                     RRRISNDEIAKRFPPGSELVVQVTKGPIGTKGPRVTANLSLPGRYLVMMPGTRLRGVS
                     RKIGDAKERQRLKKILDRLPLPDDAGLIVRTVGAGASKRAFVRDLRNLWESWNELQDN
                     IKNLRAPACVYQEPDLVERVVRDWLTEEINRVTIDDGEAYARIREVAGRISRRAKHVI
                     AQYEGQLSIFEHHGIERQLEEAFRRKVNLQSGGYLIFDETEALIAIDVNTGRHRGKGS
                     QEDAILEVNLEAVEEVARQLRLRNIGGLVVVDLIDMKSRKHQNTVYKAMKQALKRDRA
                     RTNVLPISELGIMEMTRQRQEESLLSQMYSDCPYCRGHGLIKSPLALSVDIQRQIAVL
                     MRKARHEHEGQGLVELQIVVAPSVLDRLRTEDEAFLVELQRRYTGRLTFKSELHRHPE
                     SFCIADGQSGKILYAVGEQGAR"
     gene            complement(17166..18338)
                     /locus_tag="PK576_02840"
     CDS             complement(17166..18338)
                     /locus_tag="PK576_02840"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOE60557.1"
                     /translation="MLKQILLRFQRLNPLLLTCMIALIVIGVAFVYSACSIREDPELR
                     MLYLRHAKIGCVGLVVYLALAACPFATLLHWRWVAYGACLVMLILVPFIGETTMGGRR
                     WLFGIQPSEPAKVAIILVLAQLYGHPDAKRGVGAFLVTLLVVGIPAVLILLQPDLGTA
                     MVLVPTVLAMLFAANVAPRWVWSCVLIGGVAVGLLLGMIYAAEKADLTPEKREALIAA
                     THLKPHQVRRLQVFLFPDKDVLSSGYNRRQSEMAVGSGGMRGKGYLKGEQYMLGYLPP
                     SVSSNDFIFAVLAEEAGFVGSLTVLLLFLGLFWSGLWVAGRSQDNAGRLFCVGVISLL
                     FSHMFINIAMTIGMMPITGLPLPFISYGRSFMLSMMAALGGVQSVSIHGRESGIRF"
     gene            complement(18340..20154)
                     /gene="mrdA"
                     /locus_tag="PK576_02845"
     CDS             complement(18340..20154)
                     /gene="mrdA"
                     /locus_tag="PK576_02845"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE60558.1"
                     /translation="MESGGQKQTSSWRIALLALVFAAGVAVLGSGLYRVQVVQNPSFS
                     RDQLRQSVRRVQEPGLRGRIFDRNGICLAENRASYCIAFYVEELRKRGPWIRTIDAVN
                     TEIDRLAAVLGVPRVISVAAVSNHVINSRYMPLLAWRDVGQEILARWAETQEVFPGVD
                     VYVQPERSYPQGALAAHVLGYVKRDMPLPLPGERVHFFLPEMFGANGVERQYNALLTG
                     RSGGKLIRVDARGYKHAVYQGEEAVAGQDLHLTLDVRIQRTLEQALRGLRGAGVVLDP
                     RNGEVLAMASAPAYDANAFAPYISTGLWRSLNTDPHLPLFNRALQGGYAPGSTFKPVT
                     AIAALIHGAVEPETLHTCTGVFELGTMRLHCWNTYGHGAIALRKAIEQSCNSYFCHIG
                     YTVGYEAIRTEAAKLGLGQPTGIDLPSENRGLLPTDEWKRKQLKDRWRPGDTCHIAIG
                     QGLLVTTPLQMALLTATFANYGKLCRPHVARHAPESVRDMGWPADALKWVRTGMRDVA
                     ELGTGRRVRVRGLEIAAKTGTAEFDAGGVRRKNTWVTAFAPFEQPTVAMAVMVEDGES
                     GGLTVAPIVREVLVSIFGEAPPEDAAAEPAAMPDVRGD"
     gene            complement(20123..20662)
                     /locus_tag="PK576_02850"
     CDS             complement(20123..20662)
                     /locus_tag="PK576_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60559.1"
                     /translation="MRLDTDLFRIFFALGVAALLQEMFPVIPLVPAKPGFLTAVALYY
                     ALTRPWPVALTALTWAGALTDLQDGLPLFCTIGFLLVAYGAVRLMQRLFLTATVAHGA
                     VLVAGMAAAQTLWTRLCVGVGGWSLLWQTLAAAGYAAHAGLVAGGLGFGFCGLLDRVS
                     GSERPVKEDNGIGWTETDQ"
     gene            complement(20652..21503)
                     /gene="mreC"
                     /locus_tag="PK576_02855"
     CDS             complement(20652..21503)
                     /gene="mreC"
                     /locus_tag="PK576_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOE60560.1"
                     /translation="MKQTKLWVGLLAVTLAALVFWRTGAHDFVREALYPYQKACVWFE
                     RAVWLRARAMFSRSRLAARNAMLERDVARLRMVADDVEALEAETRRLRALLDFVPPVP
                     CRWLAAPILSRGGTSAVWQQVRLGKGSLHGVRKGAPVVVPDGVVGRVMDVSLHTSDVM
                     LITDPNSRVACELETPGEGVGVVRGILYGGGSVPAAGPELTLLYVVEPLRLRYLARDF
                     EPSPHTRVVTSGLGQTFPKGLTVGFLLDSWIEPNALSREAAVMPAVDFAALDEVFVLS
                     PGGTHAP"
     gene            complement(21562..22584)
                     /locus_tag="PK576_02860"
     CDS             complement(21562..22584)
                     /locus_tag="PK576_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOE60561.1"
                     /translation="MLNKLLSFFSSDIGIDLGTANTLVYVKDRGVVIREPSVVAIQEG
                     TKRILAVGEEAKRMLGRTPANIVAIRPMQSGVIADFDITEAMIRYFIRKVHVAKVVKP
                     RVIVAVPSNITEVEKRAVQESARHAGAREVFLIEEPMAAAIGVGLPVSEPAGNMIVDI
                     GGGTCEVALISLAGIVYARSVRVGGDAMDECIVQYMRRVYNLMIGERTAEEIKITIGS
                     AYPLGEESTLEVKGRDLVAGLPKTLTVTSEEIRDALQEPVSSIVDTIRITLEKCPPEL
                     SADLVDRGLVLAGGGAMLRGLDKLIASQTGLPVTLADDPLSAVAEGTGVVLNELNFLA
                     KTKLGR"
     gene            22823..23815
                     /locus_tag="PK576_02865"
     CDS             22823..23815
                     /locus_tag="PK576_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE60562.1"
                     /translation="MARPVTIFTGQWADMPFEKVCELMSGFGYEGLEVCTWGDHIDLD
                     QASRSKTYCDEKRGLMEKYGLGCWAISNHLSGQLVCDPIDARHYAFAPAKVRNDPKAA
                     RKWAIDMQKKAAKAARNMGVTVVNGFTGSPIWHLLYSFPPCLPGQIESGYREFAKTWK
                     PILDVFADNGIRFALEVHPTEIAFDIASAQRALEALDGHPAFGFNFDPSHLGYQGVDY
                     VKFIRTFPDRIFHVHMKDAWWGHGDGTVGVFGGHTEFADPRRFWDFRSLGHGDINFED
                     IIVALNDIGYQGPLSVEWEDSRMDRCHGAQESCDFVKGVDFPPSAIAFDAQFDR"
     gene            complement(23858..>24243)
                     /locus_tag="PK576_02870"
     CDS             complement(23858..>24243)
                     /locus_tag="PK576_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60563.1"
                     /translation="RYTVRREVQCLISACLMIPAALLREHGGFDEAFHPVQFEDFDLV
                     YRLREAGHRAFVEPAAEMYHFESVTTQGTPTVRNAAAVVRNGMLFQKRWRHRFEQEEG
                     PPEEACRWRRIEPKPFDEIVDPPLF"
BASE COUNT         4777 a         8247 c         6849 g         4270 t
ORIGIN      
        1 atgtcatccg cgagtacggc gcggacagca tgcgcctgta tgagatgttc atgggtccgc
       61 tggaggcggt caagccctgg aacaccaagg gcgtggaggg cgtgttccgt ttcctgaaac
      121 gcgcctaccg gatggtcacc gaacagccga tcaccgaaga tcccctgcag ccggagcaac
      181 tccggatgct gcacgccacc gtgaaaaagg ttaccgatga tctggaggac atggggttca
      241 acaccgccat cagccagctc atgattttcc tgaacgcgtt ttccggtgcc gacaagccct
      301 tgccgcgttt ggccgccgaa accttcgtgc tgctgctgtc cccctttgcc ccgcacctcg
      361 gcgaggaact ctggcagcat ctcgggcacg aaaactccct ggcctatgca ccctggcccg
      421 tctatgacga atccctgctt cagctcgatg aggtggaaat cctcgtgcag atcaccggaa
      481 aaccgaaagc ccgtgtcatg atgccggccg atgcggatga ggaaaccatg cgtgagatgg
      541 ccctctccca atccgtgatc cgcgaggcgc tcaccggaaa aaccgtgcgc aaagtgatct
      601 gtgttcccgg acggctggtc aacatcgtgg caacttgaaa ccgcaccctg cgcgcgggtg
      661 acacagacgc ccgtcacacc gggacaaaaa gttacgcccg ttgcgttgct tttcgcgtaa
      721 gctcataggt gtcgcattgc cgtgagcggt tctcaagaca tgaacaactc ccgtggcatg
      781 ttttttgcta tgatcaatat ggcccccagg gaccagaaag ggtgtactga tgattcccta
      841 tttttatgat ccacggctga ttctcgtgat gttgcctgcg ctgattttgg cgggcatcgc
      901 ttccgtgctg gtcaaaacga ccttcaaccg ttacgcccgg gtacgggctt attcgggtat
      961 gaccggcgcg caggccgcag agcgcatgct gcgcgcgaac ggtgtgtttg acgtgacggt
     1021 cgaggaaacc ggcggattcc tttccgacca ttacgatccg acccggaagg tgctgcggct
     1081 ctcgccggac gtttaccgcg gcaactcgct ctctgcggtc ggagtcgcct gtcatgaggc
     1141 gggccacgcg ctacagcacg ccaacagcta cggcccgctg atgctccgca ccgctctggt
     1201 tcccgtgacg agtatcagca gttcactcgc cttttatgtg gtcgcggccg ggtttctttt
     1261 ccgtccgctt ctgcttgtcg ggtgcgcgat gttcgccgtg gcctttctct tctcgctggt
     1321 gaccctgccg gtcgagtgga atgccagtgc gcgggccaaa cagcatttgg tgacgtcggg
     1381 gatcgtctcg ccaggtcaag aggcggaggc cgggcgcgtg ctgaacgcgg cgttcctcac
     1441 ctatctggcc gcggcggtca gctcgctgat caccctactc tattatctgg cccgcgccgg
     1501 cgtgatcggc ggcggaagac gggattaaac aaacggagcg ttctgttttt atgagtaaga
     1561 aaacccacgt gttcaaggcg gagatccagc aggtgctgga tctcgtgatc cattccctgt
     1621 attcgaaaaa agagatcttc ctgcgcgaac tgatctcgaa cgcatcggat gcgattgacc
     1681 gtgcgcagta tctcggcctc acagacccga cgctgatcgc agatgccccg gcatggagga
     1741 tcgacattct ggccaaccgg gaggagcgga gtctgacaat cgccgacaac ggcatcggga
     1801 tgagcgaaga ggaactggac aagaacctcg gcgtgatcgc cagttcgggc accaaagcct
     1861 ttatcaaagc gttgcgggaa aaggagtcgg tggacatccc ggaactgatc ggtcagttcg
     1921 gcgtcggctt ctacgcctcg ttcatggtcg cggagcaggt cacggtcatc acgctcaagc
     1981 gcggcgccga tcaaaaggcg gtgaagtgga gttcgaccgg cgacggcgga tacacgctgg
     2041 aggatgcgga gcgggatcag cccggcacga cggtcgtgct gaaactccgg gaggatatga
     2101 aggaatatct ggacgagtgg cgcatccgcg agctggtgaa aacctactcc aatttcatcg
     2161 cctaccccat ccggttcagc gacaaaggcg agatgcccgc cgaggacagc gagccgctca
     2221 acaccatcaa ggccctttgg cgcaggccga aatcggagat caaggaagag gagtacaacg
     2281 agttttacac gcacctcagc catgatcacc ggccgccgct aaagaccatc cacttcgctg
     2341 tcgagggcgt gaccgaattc cgcgcgctgc tctttctgcc gaaagaagcg ggcatggatt
     2401 tgctcctgcc ccacaagcag cgcggtctta gtctctacgt gcggaacgtg ttcatcggtt
     2461 ccgatttcga cctgctgctc cccgattacc tccggttcgt caagggcgtg gtggattcga
     2521 gcgacctgcc gctgaacgtc tcccgcgaga tgctgcagga cgacgcgctg atccggaaga
     2581 tcaagagcaa cgtcaccggc aaaatcctcg caaccctgac cgagatgatg cgggatgaac
     2641 cggaagcgta ctccactttc tacacctcgt tcggccggat tctcaaagag ggactgcatt
     2701 gggattggga gaacagcgaa aaactgaagt cgttgctcat gttccgttcc gcgaaacagg
     2761 agaacaacgg gttcctgtct ctgaaagcgt atgtcgagtc catgcccgaa agccagaagg
     2821 atatctacta cctgctctcg gatgatctcg cgaccgcacg ccaatccccg cacatcgaag
     2881 cattgttgca gcacggttac gatgtgttgt tctttaccga tgccattgac cagtgggttg
     2941 tggagcgcct gagggaattt gacggcaaaa aactggttgc cgtggacaaa ggcactctgg
     3001 aactcggcac cgaagcggag aaggctgaaa cgaaaaaacg gatcgagacc gctgacgccg
     3061 aattccggga actgagcgcg ttcctcaaag aacacctcaa ggaaaaggtc ggcgaggtga
     3121 aactctcgac ccgtctgacc gattccgcct gctgtctcgt ggcggacgaa catgcactga
     3181 acgccagcat ggaacgcctg atgcgcgcca tgaaccaaga ggtgcccaaa caactccgca
     3241 cactggagat caacccggat catccggtgc tcaagcggat gaggcagatg ctggaaacgg
     3301 acaagtccga tccgaaactg gccgattacg cggatctgct tttgggtcaa gcgctgctca
     3361 gcgagggatc ggccctgccg gatactcaac gtttcacgcg gctggtctcc gaactcatgt
     3421 cggcgtcctg accgcaaacg cttccagcga cgagacaatc ggatagagtt gttcgtggta
     3481 ccagagcacc gagaagtaga ggccgatcgg ggttgcctgc ggtacgtttc ctgaccgcca
     3541 cgcctccttg agtctgttgc gtccgcgttc ggctgccgca cggcagggag catccccgcc
     3601 aagcagcgcg atcacagcca gggccgtctc ctcgacggtc gccgtctttc ccgcgctcca
     3661 tccaccgtcc ggtttctgac tccggatcag ccaacgctcg cccgctgtgc gcatcgcctc
     3721 cgcctctccg gcccatccca cggcatcagc ggcatagtgt gcggcacgca gcgcatccgt
     3781 cacccgcgcg gttcccacca ctggattttg tccgtgctcc gcatcctgat ggccgaacca
     3841 aagcggaatc cagctcccat caggctgctg ctcttgtttg agataaccca gaacgcgccg
     3901 gatggcggaa tccaccttac gctccgcgta cgacggaaga gccgggtcgt tgcgccaggc
     3961 cgcccatgcc gccaaggcgt gcgcactgat atcgggacag ctccggtcga acggcaaccg
     4021 gccccatccc cggcagaacg tgggaagccc cccatcccga ttctgcaagt ccagtaacca
     4081 ccgtacgcca tcgcggaccg cagcgcgaaa atcgaccgcg catccggcgt cccgcaggcg
     4141 tttgagtgcc accagcgcac ctgccgtatc gtccgcgtcc ggcacaccgc ccggcagatc
     4201 cgtccatgcc cagccgccgg gcgccgcacc agtgaagggg tgacgttctg tcttctgcac
     4261 ctgaatcaac cacctcgcga tcggcgtcag cgcctcggcc tccacccttc ccgcacccgc
     4321 cccctcccga tacgcggtta tcgcgcgcgc cgccagcgat gtcacccaag tgcgcagatt
     4381 ggagtcgatc gcccagcctc cgcccgcgcg cgccgaatgc cgcaggaacg caagtccctt
     4441 gcccaccacg gcgtgcccgc cgaaaccggc gtgatgcaac gccaagacca cgaaagcggt
     4501 cagcggaatc gcatccagat atccgccgtg gtccggttgc agcgcggcca gttttcgcag
     4561 cagcgcggga gcgagaacac gcccccatgc cgcaatccgc cggttccgcg ccacacgcag
     4621 atggcggcac agccccacag cgatcaaggc gggcagcgcg taactcacca cctgcaaccg
     4681 gagaagccga tagagtcgct gcggcagaca ggccagcgca aaaggcaggg gcatgaccca
     4741 ttgccaagcc tgccgcgtgt cctgacacat ggcgagaaag gcctggatcg gcaccgcaaa
     4801 ggtccgatcc tccccgtagg tgcgacgcaa cgcgcgcgcg accgaagcgg gtgaccgcgc
     4861 ttcgccaagc tgccggtcga tccactctcc ggcgcgagcc accgttttcg catgacgctc
     4921 acccgcaaac cgttgcagtg ccgcatgaac caacagggtc gtgctcagat tcgaccggct
     4981 gtgcggggta tccccccacc cgccgtccgg attcacgtga cacgccagcc aattcaatcc
     5041 ggaatccgcc tgcacccggt cgtcggcctc ccccgcatcc gccaaggctg cggcagccag
     5101 cgccgtggcc aaagcgctct ccgccagctc cccgctccag cttccgccgc cggggtcgcg
     5161 caagcgccag agatgacaaa agacctgctg accttcctcc ccgtccgcag catcgcatcg
     5221 gttcttcatg attggaagtg tagcatgtcg tgactgcggc gacgagcttt ttcaggcctt
     5281 gccgaatgtc gacgtacgcg aaaacgagga cggggacgag gacgaacccg aacccgatag
     5341 cgataaaccc ggaatcagga gcccgaacgg acttgccccg ttgaaacgtt tcaactatga
     5401 tgacttcaaa acccggatgg tatgaatgtt gcttacggcc cgcaggccca agagtccggg
     5461 agaggatcat tatggctgtc ccgtccctga gtttaagtca gcagcagcgt caggtcatga
     5521 cgctggcgcc gcaacttcgg caatcgttgg aaatgctcca gatgccggtg atggagttgc
     5581 gcgcggcgat cctcaacgaa atgtccgtca accccacgat cgaggaggtg gaggatccca
     5641 gcgaagtgtc gctggccacc gtgaccgcgc cggaaccgca gcagcagagt gcggctgatg
     5701 aggaactgga tttcaatccc gatatcgacg cgatcctgcg gcaagatgac gagtggcgcg
     5761 actacttcat gcagggcatg gaaaatgccc cctcgtacga ggaagagaat gaaaagcggc
     5821 aatacctgct ggactcgatc cgccagaagg cttcgctgca ggattatctg ttggagcagc
     5881 ttacgctcac ggatctccag ggcgaggact atgatctcgc agtgctgctg atcggcaaca
     5941 tttcggatga cgggtatttc accggaagcc tgccggacat catcatggtt tcgggccgca
     6001 gcgagcgcga agtgctcgat gtgctcaagg tcatccagac gtttgatccg ccgggcgtgg
     6061 gagcccgcac gctgcgcgaa tgtctgcttc tccaactcgc gctggttgaa gactccccct
     6121 gggaggatga ggcccggctg gtcatcgaca agcatctcga gcggctcggc gcgcatgacg
     6181 agaagtacct ctgcaaggtg cttgaactta cccgggagga gttggcgcat gtgaccgccc
     6241 tgatccgcag cctgaacccg cgtccgggac gggcgtatgt tcaggaaaag acggagtatg
     6301 tggagccaga ggtgtttctg gtccgcgaga aggggcgtct ggtcgcccgg gtcgacaacc
     6361 agaggctgcc gcacatccac atcagcaagc actatcggag tctgctggag gaccggaaca
     6421 ccccggcgga gaccaaaacc tacatccgtg agcgcatccg tgcgggcgcc tttcttatcc
     6481 ggagcctgta tcagcgccag gagactatcc ggaaaatcgc ccaggagatc gtggatgcgc
     6541 aggctgattt ctgggaaaag ggtgtgatgg cgctgcggcc gttgaccatg gccgaggtgg
     6601 cccgcaaggt cggggttcac gagaccacgg taagccggac ggtctccaac aaatacatgc
     6661 gcacaccacg cggcgtcttt gagatgaagt atttcttcac gcacgggttg aagtccgaag
     6721 acggggtgtc cgtctcgaac aaatccgtgc aggatcagat ccgccggctg gtggaggcgg
     6781 aagaccctac ggccccgctc tccgatcagg ctctggaaga gaagctttca gagcagggca
     6841 tcaaggtcgc acgccggacg atcgccaaat accgcggcat cctgaagatt ccgccctcgc
     6901 acaagcggcg ccgcacctga acacaccgaa gcgagtcgcg gaagttgnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnntgc ggacgcctcg gggatgcgtc cctaccggca
     7081 acctcaacgt tcggcgctcc cgccttctgg gggcttcggg ctccgccccg gttagtcgat
     7141 gtagaggtca ggcggcggag agacggtctc gaaaaccggc gcgggaccct cgctcactcc
     7201 cgatccggtc agccggaaca gcaggctgat cccggccaga aggcagatca acaccaccac
     7261 accggcaccc gccagcatca gcacgctttt atgcggcagg gtcacgggca gacggcgccc
     7321 cacatctgtc cgcagcttca tgaacccctc tttaaacgcc cggatgcgcg cattccgccg
     7381 ggcgtgggcg tcagagtcct catccgcgaa cccgcctgga gcgcggtcat tggctcttcc
     7441 gccgattttg aagatcggca acacgcgccg ttgggcaacg gcgaatcggt cggcactctt
     7501 gcgtttttcc gcgtctgcgg catccggctg acgcggggta acggacggcg cgggatggaa
     7561 gagatccggt tcctgcggca catcccgctc tggcttctcc tcctcctctg gccgcaccac
     7621 ccacccgaac agatcgtgcg caccgtggcg ggctgaccgg acttcccgtt ccgcgcccgt
     7681 ttcctccgtc ctggacttga ccgcaaccct gtccgcatct tcggcaggcg gtgctgccga
     7741 ttccggcgct tccttcaccg gccgcaccat cacggcggga cggggctcaa tccgctggcg
     7801 ctgcggcccg gcctccggtg cctgacgcgg cgtaaccgcc gacgcggttt ccgccgcatc
     7861 ctgctcggcc tcatggggca cgtcgcgggt tcggatggca ggcacacgcg caccagtgta
     7921 caagtccatg aattcgcgga tcagcggctc gggatccaat tccagcagtt ccgcataaag
     7981 cttgataaag ccgcgaccgt aaatcggcgc cgccacccgg ctgaactgct cacgttccag
     8041 atcctcaacc acctgcacca gcagatgcgt gctctccgcc acatccgatg gcgtcagccc
     8101 gcgttgcagg cgcgcgttct tcagaatctc tccgaatgcc atggtgacct cgctttcctg
     8161 ttacacgcgg ggcgattcgt ccgcctccgc ctcctcgtcc ggcaacggag cgcccgcacg
     8221 atccgtgcca ccgcccaccg gaatttcctt gtccaaatca ataagaatct cacgcgagtt
     8281 ggagcccgcg tgcggaccca ccacaccgcg ctcttccaga atgtcgatca ggcgggcggc
     8341 ccgcgtgtac ccgatgcgca accggcgttg cagcatggtg gtcgacgcgc gccgcgtcat
     8401 gcggatcacc tcgatcgcgt tcggcagcag cacgtcatcc gactcgccgt cctccatccc
     8461 ctgctcactt ccgccctcgc cgtcctcctc atccgacaac gccgctccag gctcgctctc
     8521 cctgattttt tccagccggc ccgagagcgc ggcatcgtaa cagggcgcgg attgctcttt
     8581 gacgtaggcc acgacacgcg cgatctcctc atccgagacc cacgcgccct gcaggcgcgt
     8641 cagttgcccg ccctcccgca aaaacagcat gtcgcctttg ccgatcagct cttccgcacc
     8701 cgggctgtcc agaatcgtgc ggctgtcgtt ggactgcgac accttgaagg caatgcgtcc
     8761 gggaatgttc gacttgatcg tcccggtgat cacatccacg gacgggcgtt gcgtggcgag
     8821 gatcaggtga atgcccaccg cacgcgaaag cgaggtgagg cgcgcgatgg ccggctccac
     8881 ttctttgccg accgacgcca tgatgtccgc cacctcatcc acgacaatca cgatgtacgg
     8941 caaccggtcc ggcgtctcat cgactgcgga cgcctgcgga gaaagtgcgt cctcctcccc
     9001 gaacagatca tcctgccgag cgatcacccg gctgttgtac gccacgatat tacggcaccc
     9061 ttccttctga agcaaccggt accgcttctc catctccatg atggcccacc gcagaccgaa
     9121 cgccactttc ttggggtcgt tgatcaccgg caccagcaga tggggcaact cgttgtaaca
     9181 ggtgaactcc acgcgcttcg gatccaccag aatcagccgg agttgatccg gcgtgcggct
     9241 catcagaaac ccggtgagta tggcattgag gcacacgctc ttgccggaac cggtagcccc
     9301 ggcgaccagc agatgaggcg ctttcgtgag gtcgaacacc acatcgtccc ccgcaacgtc
     9361 cttgcccaaa agcagcggca cctcgcaggt gttgcgggtc caggcctcgc cctcgatgat
     9421 ctcgcggaag gtgatcgcgc gggcgcgacg gttgggaatt tcgatgccca ccgcattctt
     9481 tcccggaatc ggggcctgaa cccgcaggct ggtcgcttcc agcgccatct gcaagttggc
     9541 ggtcaggttt gcgatgcgtt caaccttgat gttcggcgcg gggcgcagtt catattgcgt
     9601 caccacaggg cccggagtgg taccgaccac ctccaccggc acgttgaatt gactcagggt
     9661 ttcgaccaga atatcggagg tttcggtgat gttgccatga tccgccgccc ctttggggat
     9721 cgggttgagt aacctgaccg acggcagctc gtaggcggcc gggagcgttg cggcaggttc
     9781 cggttgcgcg acaacggcag gcgcgggccg ggtccccgcc gccttttccg gcacgacctg
     9841 ttggggctcc acgcgcgggg cgggagcggg ggcggccgga gcgggctcgc gctggcgctc
     9901 ttgctcacgc gcctgacgcg cctcctcttt ggcccggcgc gcggcctccc ggattgcctg
     9961 acgctgctga cgctcccgct cgcgacgcgt aagcccgtca tccgcctccg aatcttcctt
    10021 ttccgccgta ccggggggtt ccgtcttgtc tgcggcacgc ggccgcgtcg tccaccaacc
    10081 ggtgacccat cccaacccgg ccacgatatt ccgcaacccc accgccagaa acagggagca
    10141 cagcataatg gagagcatca ggatcccgcc acccaccgga ctgaaccagc gagccaacag
    10201 gcaggtcatc agcacatatc cgaccgcccc gccggcattc ggcttcaggt tgaggcgttc
    10261 cagaacagga tcaaacactg tcgcgctacc aagttgcagc aaacagcaaa gcgcgatcag
    10321 gaaaatcagc atccagaagg cccggcggac aagacgcacg gcacgcccgt agatcagcat
    10381 gacgctgaac agcagcacca tcaccggaac cagccaaagc gcgagaccga ccagactgta
    10441 gccgatgaac accgaccaag cgccgacgat gccgatcaga ttcgaggggg gcgtcagggg
    10501 cggcgcctgc aaccaggaga tgtcgcgcca attgtatgtc agaagactca gcagcggaaa
    10561 gagcgcaatc ggaaagagca acacaccggt ccaacgccgc tgaatgcgtc gctccaactc
    10621 catcctgtcc tgttgttcta tcaaccgttc cgacatggct ctcacttttg ccccgcgggc
    10681 gtatcggcgc cgacaaccga cgctgtcacg ggaatcaggc ggaccgtcgg cggcaacgcc
    10741 cggacctcgc tgacctctaa tccctgttga aggtgtatca tgaccggcac cccgttggtc
    10801 tcccgcgaca gcgggagatt accgctgacc acggcggtca cctgctccgg cgtgaccgcc
    10861 ttgacgatct cgccgcgtcc gctcaactcg acctccacac actccggctc cacgtgccag
    10921 cgctgcacca tgtcatgcgg gtataccacc accggcacgc gctccagttt cgcggtggcc
    10981 ttctcgctga ggatctgcac ctgcaccacc atttcgggcg gttccacggt cgcgttcacc
    11041 acatcgccgg gcggcaacag gcgcacacgc gtcgaaaagg tttgcgagcg gccgtccaca
    11101 ttgatgggtt cggtttggat ctgggtgacc tcgggattca gcgtgctgag ctggcggcgc
    11161 gaaccccgca caaccgcatt ggtgaggtcg tacaccaact gcacgcggcc acgcgccgtg
    11221 ccgatctcgg cgggtcgcgc gatcataagc ggaagggaca tgggcacgtc aaacttgacg
    11281 accgccacgt tcggatcaac cttcaccacc cggatcccgc gcaccccgcg cagattgccg
    11341 cgccggattt tgaccggcac cgagtccacc agattcccct tcttctgccg cggccgcacc
    11401 acgcaacgcg ccacagagag cgacaactgg ttcacatccg tataggagcc gcgcagggtc
    11461 acctgcacgg aacgcggctc cacggattca atcgcggcac cggcacccgc cgcatcgaac
    11521 tcggcctcca ccggaatcga gatgacccgc acatgactga tttcggcccg gatcgagaaa
    11581 tagaccagaa cggcgatcac caacgagagc aacttccaat acccgttgtt gagaaacacg
    11641 atccgcagga tcgaaagcac cttatccctt tgcatgatcg tcccccccga ccgacatcgc
    11701 caacggcttc aaccactttt cgagtttcag ggtcagccat tcggtcacgg tcgacgcgtc
    11761 ccctgtctgc tgcggcatgg ccacccgcaa ccagcgcaga atcatcttac cgatcttgtc
    11821 atcctcatac tgatgcagtc tgccgttgta ggccgcggaa atacgtccgg tctcttcgga
    11881 caccacgatc accacggcgt cggtctcctc gctcagcccc actgcggccc gatggcgcat
    11941 tccggaagcc gcgagttcac cctgattgga taccggaaaa agacagtgcg cagaggccac
    12001 ccggccatca cgcagaatga tgccgccgtc gtgcagaggc aacggcgggg tgaaaaggga
    12061 gaccatcagt tcatgactga acaaggcgtt cagcttaacc cctgtctcct catacccgca
    12121 cagcgaaatg ccgcgttcca ccgcgatcag ggcaccgatc cgcgcccgcg acagattcat
    12181 cacgctctgg cagatcaact cgggcacaga ggactgctgc cgcgtgtact ggcgtgagaa
    12241 caaccgttgt ccgccgacca gcgccagcac acggcggatc tcctgctgaa acaccaccac
    12301 catactcagc gccagataga tcaggaaaaa atacaccacc cacgccagca catcgaagcg
    12361 gaagagatag gtgaacagca gcaaagagac caccagaacg cagaacccca acaacacctg
    12421 cgcactgcgc gtgcctttca cgaaacggat cagcgcatag atccccagat agaggatcag
    12481 gatctgcatc acgtcgttga agccgatctg gacaccatca aacatgcgtc tcacccactg
    12541 ttgcctccgc cagacggcgg accatcgcca acgcctggcg ggtagccttc acgtcatgca
    12601 cccgcaccac cgcggccccg tgcaacgcgc accagaccgc cgcacccaca ctgccgccca
    12661 cacgttcacg cgcgtcaggc tcgccgcaca cctcgccgac gaaacgcttg cgcgaggcgc
    12721 ccaccagcaa cggccccaat cgcgacaggc gcgcggtcgc ggccaacaac gcgacggaat
    12781 gcgccgtgtc cttcgcaaac ccgataccgg gatccagaag aatccgctcg cgcccgatcc
    12841 cgctttccac cgcacacgcc aacgcatcac gcagcattgc ctccacttcg cccaccacgt
    12901 cgcggtattc ggtcaggtca gacatggtgc ggggcgtccc gcgcatgtgc atcagcacca
    12961 cacccgcccc gctgtcacgc accagacgaa ccatctccgc gtcttccgtg aacggctgca
    13021 cggcattgac cgcacaggct cccgcctcca gcgccgcgcg ggccaccgca gcatggcgcg
    13081 tatccaccgt aaccggcacg ccgctccgct ccgccagcat ccggatcacc ggcgccaccc
    13141 ggcgcgtctc ctcttccgtg ctgacctctt ccgcgcccgg ccgcgtcgat tcgccaccga
    13201 tatcaaggat atccgcccct tcctccacca aatccagtgc gtgggccacg gcgcgttcct
    13261 cgtcgaacca cttgccgccg tcggaaaagg agtccggcgt cacattgacg atgcccatca
    13321 cgagcgtatg gcgcaacggc accacgcacc cgcggcacac ccatgtgcgc ccgctgtctc
    13381 tctccacact ctgcactccc atcctctcgc gtacgactac agtagaaact ccggcggctc
    13441 atccggctta tcctgcggcg tttccttgtc gacaggaaca gcgggatccg ttgatgccgg
    13501 ttcgtctgcc tgcccgtcct ccagcgacga cacctcgtcg ggcaggcggc cttgacggat
    13561 caggctctcc acatcgcggc cgtccagcgt ctcggcctcc aacaggcact ccaccaagcg
    13621 ctccagtttc gcgcggttgt cttgcagcat ccgttccgct cgcgcgtacg actcggtaac
    13681 cagccgcgag acctccgcgt cgatttcctt ggcggtctcc tcgctgtagg cttggttgcg
    13741 cgccacttcg cgccccagaa agagcgtctc ctgattgtca ccgaatgact ggaaaccaaa
    13801 ccggtcgctc ataccgtacg aacagatcat tttccgcgcg atctcgcttg ccattttgat
    13861 gtccatgcgc gcgccggtgg agaggtcggc ggtgaagaat ttttccgcga tgcgtccacc
    13921 catcagaacc accaactggt cgagcaattc gctgcgcgta cggttcagca catcccgctc
    13981 gggaagggac atcgtggccc ccaaggcctg gccgcgcgga atgatcgtga ctttgtgcag
    14041 cggctcggca tgttcgcaca gcacttgcag caacgcgtgg cccgactcgt gccaggcggt
    14101 gatgcggcgc tcgcggtcat ccatggcccg gctgcgacgc tcgcggcccc acagcacctt
    14161 gtcgcgcgcc tcttccagat cggactgccc gaccgcccgc ttgttggcgc gcgccgccag
    14221 cagcgccgcc tcgttcagca ggttggccaa atccgcaccg gagaagccgg gcgtcccgcg
    14281 ggcgattcgg ctcaggtcgg cgtcatgcgc caacttgatc ttctcggcgt gcatccgcag
    14341 gatctcctcg cggcccttca gggtgggcag gtcgatgatg atctggcggt cgaagcggcc
    14401 cggccgcagt aacgccggat ccagcacatc ggcgcggttc gtggccgcaa tgatgatcac
    14461 gccttccgag gtctcgaaac cgtccatctc caccagcagc gcgttgagcg tctgctcgcg
    14521 ctcatcatgc ccgccgccga taccggagaa gcggctgcgg cccaccgcat cgatctcatc
    14581 gatgaaaatc aggcagggcg catgcttctt gccttgctcg aacatgtcgc gcacccgcga
    14641 cgcgcccacc cccacgaaca tctccacgaa atccgaaccg ctgatactga agaagggcac
    14701 gcccgcctcc ccggcgatcg ccttggccaa caaggttttg cccgttcccg gcggccccat
    14761 gagcagcacc cccttgggaa tccgtccgcc gaggacttgg aatttccggg ggtctttcag
    14821 aaactcgacg atctcctgca cctcttcctt ggcctcccgg atgcctgcca cattcgcgaa
    14881 cgtcagcttc ttcttgtcct ggctcaacat tttcgcccgg ctcttgccga aactcatcgc
    14941 gcccatgttg gccgatttga tttggcggat gaaaacgaaa tagaggatca ccaggatcag
    15001 gatcaccggc aggatgtcgc ggagcagcgc gccgaagaca ttggtgttgt aaaccaacgg
    15061 acagggaatc ttcttttcga gcagaaactg ctcgagattc tcggacggga tcaggttgac
    15121 acggaaccgc tctttggcgg cacccttgcg gactccccgg aggtaggtca tgccgttcgc
    15181 ttcgcgcacc aactcgacct tttcgatcag tccctcttcc accagtcccc ggaactcgag
    15241 ctggtccatg ggccgcatcg cgccttcggg tttgtaatag aacatcccca gcatgaacag
    15301 cagcaacagg ccgacgacca tccaggcgcc gaaaacccgt ttgggaggca gggcgccgct
    15361 tgtcgcgccc ccctccggct tgcccccccg gttcgggagc gcgggaggtt gattcggcag
    15421 ctcgcgcttg tgatcactct ctttcgacat agggttcata tcatacgttc ccaacgcgcg
    15481 tggggcaatg cgaaaacagt ggaacgttca ccgcgaacgc ccccatcacc tcgcgccctg
    15541 ctcgccaaca gcgtatagga ttttcccgct ctgcccgtcc gcgatacaga aagattcggg
    15601 atggcggtgc aattccgact tgaaggtcag acggccggtg tagcggcgct gcaactccac
    15661 gagaaacgcc tcgtcttccg tgcgcagacg atccagcaca ctcggcgcca ccacgatctg
    15721 cagctccacc agaccctgtc cctcgtgctc gtgacgggcc ttccgcatga gcaccgcgat
    15781 ctggcgctgg atatccacac tcagggccag cggtgatttg atcaggccgt ggccgcggca
    15841 atacggacag tccgaataca tctgggacag cagactctct tcctgacgtt gacgcgtcat
    15901 ctccatgatc cccaactccg agatgggcaa cacgttcgtg cgggcgcggt cgcgcttgag
    15961 tgcctgtttc atggccttgt agaccgtgtt ctggtgtttc cgggatttca tgtcgatcag
    16021 gtccaccacc accagaccgc cgatgttgcg caaccgcaac tggcgcgcca cctcttccac
    16081 cgcctcaagg ttcacttcga ggatggcgtc ctcctgcgag cccttgccgc ggtggcgtcc
    16141 ggtattcacg tcgatggcga tcagcgcctc ggtctcatcg aagatcagat acccgccgga
    16201 ctggaggttg accttgcgcc ggaacgcctc ctcgagttga cgctcgatgc cgtggtgctc
    16261 gaaaatcgac aactgtccct catactgggc gatcacgtgt ttggcccggc gcgagatccg
    16321 gccggccacc tcccggatac gggcgtaagc ctcgccgtca tcaatcgtca cgcggttgat
    16381 ttcctcggtc agccagtcgc gcaccacgcg ctcgaccaaa tccggctcct gatacacgca
    16441 ggccggcgcc cgcaggttct tgatgttgtc ttgcagttca ttccatgact cccaaaggtt
    16501 ccggaggtcg cgcacgaaag cgcgcttgct ggccccggcc ccgaccgtgc gcacgatcag
    16561 gccggcgtca tccggcaacg gcagccgatc cagaatcttc ttcaggcgct ggcgctcttt
    16621 cgcatcgccg attttgcgtg acaccccgcg taaccgcgta cccggcatca tcaccagata
    16681 ccgtcccggc agactcaggt tggcggtcac gcgcggcccc ttggtgccga tcggcccctt
    16741 ggtcacctgc accaccagct ccgaccccgg gggaaaccgt ttcgcgatct catcattcga
    16801 gatgcgccgg cggcgcgagg gacgcacgtt ctcctcgtcg tcatccagac ggctgtcgtc
    16861 atccggcagc atgtcccagt agtgcaggaa cgcatttttc ttcatgccga tatcgacgaa
    16921 cgcggcctgc agatcatgct ccagattctg gatgcggccc ttgtaaatgc ttccgaccag
    16981 acgttcctcg gtgggatgct ccacctgaaa ctcctccacc cggccgttct ccacaaccgc
    17041 cacgcgcgtt tcgagcttct ccacattcac cacgatctca cgcttcggcg cgcctttttt
    17101 aaaccaaccg aataacatac ccaacctcct gactggacgg ggattccgcg cacagcgcgc
    17161 cccgctcaaa accttatccc tgattcacgt ccatgtatgg acacactctg gactcccccc
    17221 agcgccgcca tcatcgacag cataaaggaa cgcccgtaac tgataaacgg caacggcaac
    17281 ccggtaatgg gcatcatccc gatcgtcatc gcaatattga tgaacatgtg actgaaaagc
    17341 agggagatga ccccgacgca gaacaagcgc cccgcattgt cttgcgaacg gcccgcaacc
    17401 cacagcccgg accagaacag gcccagaaac aggagcagaa ccgtcaacga acccacgaaa
    17461 ccggcctctt cggccaatac cgcgaaaatg aaatcattgg acgaaaccga cggcggcaga
    17521 taacccagca tatactgctc gcctttcaga taccctttgc cgcgcatgcc gccggacccg
    17581 accgccatct cggactgccg ccggttatag ccgctgctca gcacgtcctt atccggaaac
    17641 aggaagactt gcagacgccg gacctgatgc ggctttagat gcgtcgccgc gattagggcc
    17701 tcgcgcttct ccggcgtgag gtcggccttt tccgcagcgt aaatcatgcc gagcaacagt
    17761 ccgactgcga cccccccgat caacacgcag gaccagaccc aacgcggtgc cacatttgcc
    17821 gcgaacagca tcgcgagcac ggtcggcacc agaaccatcg cggttccgag atcgggctgc
    17881 agcaggatga gcaccgccgg gatgcccacc acgagcagcg tcaccagaaa agcgcccacc
    17941 ccccgcttcg cgtccggatg tccatagagt tgggcgagca ccagaatgat cgccaccttg
    18001 gcgggctcgg acggctggat accgaacagc cagcgccgac cgcccatcgt ggtctcgccg
    18061 ataaacggca ccaggatcag catgaccaga caggcgccat acgccaccca acgccaatgc
    18121 agaagcgtgg cgaacgggca ggcggcgagc gccagataca cgaccagccc cacacagccg
    18181 atcttcgcgt gccgcaggta gagcatccgg agttccggat cctcccggat cgaacaagcg
    18241 ctgtacacga acgccacgcc gatgacgatc agcgcgatca tgcaggtgag gagcagaggg
    18301 ttcaggcgtt ggaaccgcag caggatctgt ttaagcatgt cagtcccccc tcacatccgg
    18361 cattgccgcc ggttccgccg ccgcatcctc cggcggcgcc tccccgaaga tggacaccag
    18421 aacctcacgc acgatcggtg ccacggtcag gccgccggat tcaccgtcct ccaccatgac
    18481 cgccatcgcc acagtgggct gctcgaaggg cgcgaaggcc gtgacccagg tgttcttgcg
    18541 ccgcacgcct cccgcgtcaa actcggccgt gccggtcttg gcggcgattt cgagcccccg
    18601 cacccggaca cgccgtcccg taccgagttc cgccacatcc cgcatccccg tgcgcaccca
    18661 cttcagcgcg tcagcaggcc atcccatgtc ccgcaccgat tccggcgcgt gccgcgcgac
    18721 atgcggtcgg caaagcttgc cgtagttcgc gaaagtcgcg gtgaggagcg ccatctgcaa
    18781 cggcgtggtg accagcaaac cctgcccgat ggcgatatgg caggtgtcgc cgggccgcca
    18841 ccggtccttg agttgcttcc gcttccactc gtcggtcggc agcagtccgc ggttctcgga
    18901 gggcagatcg attccggtcg gctgtcccag ccccagtttg gcggcttcgg tgcggatggc
    18961 ctcatagccg accgtatatc cgatgtggca gaaatagctg ttgcacgatt gttcgatcgc
    19021 cttgcgcagc gcgatcgcgc cgtggccgta ggtgttccag caatgcagcc gcatggttcc
    19081 cagttcgaac acgcccgtgc aggtgtggag cgtctcaggc tccaccgcac cgtggatcag
    19141 ggccgcgatg gcggtaaccg gtttgaatgt gcttcccggc gcgtatccgc cctgcagcgc
    19201 acggttgaac agaggcaaat gcggatccgt gttcaggctc ctccaaagtc ctgtggaaat
    19261 atacggcgca aacgcgttcg catcgtatgc gggcgcgctc gccatggcca gcacttcgcc
    19321 gttgcgggga tcgagtacaa cccccgcccc ccgcagaccg cgcagggcct gctccagtgt
    19381 gcgctggatc cgcacatcaa gtgtcagatg gaggtcctgc cccgccaccg cctcctcgcc
    19441 ttgatacacc gcatgcttat acccgcgggc gtccacgcgg atcagcttgc cgccagagcg
    19501 cccggtgagc aacgcgttgt actgccgctc gactccgttc gcgccgaaca tttccggcag
    19561 gaagaagtga acgcgctcgc cgggcaacgg caacggcata tcgcgcttga cgtaacccag
    19621 cacatgcgcg gccagcgccc cctggggata ggatcgctcc ggctgcacat acacgtccac
    19681 ccccgggaac acttcctgag tctcagccca gcgggccaga atttcctgcc ccacatcgcg
    19741 ccacgccagc aacggcatat accggctgtt gatcacatga ttcgaaaccg ccgcaaccga
    19801 gatcacgcgg gggaccccca acactgccgc gagccggtcg atctccgtat tcaccgcatc
    19861 gatggtccgg atccacgggc cgcgtttccg caactcctcg acataaaagg cgatgcagta
    19921 actggcgcgg ttctcggcca ggcagatgcc gttccggtcg aaaatccggc cgcgcaaccc
    19981 cggttcctgc acgcgccgca cactctggcg gagttggtcc cgcgaaaaag acgggttctg
    20041 caccacctgc acccggtaca acccggaacc caggaccgcg acacccgccg cgaacaccaa
    20101 tgccagcaat gcgatacgcc agctactggt ctgtttctgt ccacccgatt ccattgtcct
    20161 ctttcaccgg tctttcgctt cctgagacgc ggtccagcag accgcaaaaa ccgaatccca
    20221 gcccccccgc aaccagtccc gcatgagccg catacccggc tgccgctaac gtctgccaca
    20281 ggagggacca accgccgaca ccaacacaca atcgcgtcca caacgtctgc gccgccgcca
    20341 tccctgccac cagcaccgcg ccatgcgcca cggtcgcagt cagaaacagc cgctgcatca
    20401 gccggaccgc accgtacgcg accagcaaaa atccgatcgt gcagaacagc ggcagcccgt
    20461 cctgcagatc ggtcagcgcg cccgcccacg tcagcgcggt cagcgccacg ggccatggcc
    20521 gggtgagcgc gtaatacaac gccaccgcag tcaaaaaccc gggtttcgcg ggaacgaggg
    20581 gaatcacggg gaacatctct tgcagcagcg cggccacacc gagcgcgaag aagatccgga
    20641 acagatccgt gtcaaggcgc atgcgtacct cccggggaaa gcacaaacac ctcgtccaat
    20701 gccgcaaaat ccactgccgg catgactgcg gcctcacgcg acagcgcgtt cggctcgatc
    20761 cagctgtcca gcagaaaccc gaccgtgagc cccttcggga aggtctggcc caaaccggag
    20821 gtcaccaccc gcgtgtgggg ggacggctca aaatcccgcg ccagataccg caaccgcaac
    20881 ggctccacca catacagcaa tgtgagttcg ggaccggccg caggcacaga ccctccgccg
    20941 tacaggatcc cgcgcaccac ccccaccccc tcgccgggcg tttcgagctc acacgccaca
    21001 cggctgttgg ggtccgtgat cagcatcacg tcgctggtat gcagcgagac atccatcacc
    21061 cgccccacca caccgtccgg caccacaacc ggcgcacctt tgcgtacccc gtggagcgat
    21121 cctttgccaa gccgcacctg ttgccagacc gccgaggtgc cgcctcgcga aaggatgggg
    21181 gcggccagcc aacgacaagg caccggcggc acgaagtcga gcagcgcgcg caacctgcgg
    21241 gtctccgcct ccagcgcctc gacatcgtcc gccaccatcc gcagacgcgc cacgtcgcgc
    21301 tccagcatgg cgttccgggc cgccagccgg gagcgcgaaa acatggcgcg cgcgcgcaac
    21361 catacggcac gctcgaacca gacgcaggct ttttgatagg gatagagggc ttcgcggaca
    21421 aaatcatgcg cgcccgtgcg ccaaaaaacc agcgccgcga gcgtcaccgc caacagtccg
    21481 acccacaatt tcgtttgctt catttccggt caccccccgt tttgccgggc aggcagagac
    21541 cagaaagcag ccgaagtcag atcaacgtcc caacttggtt ttcgccagga aattcagctc
    21601 gtttaacacc actcccgtgc cttccgcgac cgcgctcagc gggtcatcgg ccagcgtgac
    21661 gggcaatcct gtctgcgacg cgatcagctt gtcaagcccc cgtagcatcg cgccaccacc
    21721 ggccagcacc agaccacggt ccacgagatc cgccgacaac tcagggggac acttctccag
    21781 cgtgatccgg atggtgtcga caatcgagga caccggctcc tgcagggcgt ctcgaatctc
    21841 ttccgacgtg accgttaaag tcttgggaag cccggccacc agatcgcggc ccttgacctc
    21901 cagtgtgctc tcctcaccta aaggataagc cgaaccaatc gtgattttga tctcctccgc
    21961 ggtccgctct ccaatcatca gattgtaaac tctccgcatg tactggacga tacattcgtc
    22021 catggcgtca ccgccaactc gcacactccg cgcataaacg atgccggcca gcgagatcag
    22081 cgcgacctcg catgttccgc cgccgatatc cacgatcatg ttgcctgccg gttccgaaac
    22141 cggcagcccg acaccgatgg ccgcggccat cggctcctca atcagaaaaa cctcgcgtgc
    22201 gcccgcatgc cgcgccgatt cctgcacggc gcgcttctcc acctctgtga tgttgctcgg
    22261 cacggccaca atcaccctcg gcttcaccac cttggccaca tgcaccttgc ggatgaaata
    22321 ccggatcatc gcctcggtga tgtcgaagtc ggcgatcacc cccgattgca tcggacggat
    22381 cgcaacgatg ttagccggcg tgcggcccag catgcgcttg gcctcctcgc ctaccgccag
    22441 aatccgcttg gtcccctcct ggatcgccac cacagagggc tcccggatca caaccccgcg
    22501 gtctttgaca taaaccagcg tgttggcagt acctagatca atcccgatat cacttgaaaa
    22561 aaaagacaac aacttgttga gcatctcgcg ttctttcccg tgggacaaac gtttaaatgg
    22621 tgctacaatg aagcagtgtc gatagtttcc acgatccgct ggaacgggtc aagccccaat
    22681 tctaactttt tgcatatatc cgccggattg ctcttgatcg ttgccgccgc ctgcggtatg
    22741 gttacccgca taacctctgt tgccgggtgc tgtgccaatc ggcgcgtccg ctccgcggca
    22801 acgcgaaagg agtattccga ttatggcaag acctgtaacg atcttcaccg ggcaatgggc
    22861 ggatatgccg tttgaaaaag tttgcgagct gatgtccgga ttcggatatg aaggactcga
    22921 agtgtgcacc tggggcgacc acatcgacct cgaccaggcc tcccgaagta aaacctactg
    22981 cgatgagaaa cggggtctga tggagaaata cgggttggga tgctgggcca tctcgaacca
    23041 tctttccgga cagttggtct gcgacccgat cgacgcccgc cattacgcat tcgcgcccgc
    23101 caaggtgcgt aacgacccca aagccgcccg caaatgggca atcgacatgc agaaaaaagc
    23161 ggccaaggcc gcccgtaata tgggcgtgac cgtggtgaac ggcttcaccg gctccccgat
    23221 ctggcacctg ctctactcgt tcccgccctg tctgccgggc cagatcgaga gcggttatag
    23281 ggagttcgcc aaaacctgga aaccgattct cgatgtcttc gcggacaacg gcatccggtt
    23341 cgccctcgag gtgcacccga ccgaaatcgc ctttgacatc gcgtcggcgc agcgcgcgct
    23401 ggaggctttg gacggccatc cggccttcgg gttcaacttc gacccctccc acctcggcta
    23461 tcagggtgtg gattacgtga agttcatccg caccttcccg gaccggatct tccacgtcca
    23521 catgaaggac gcgtggtggg gccacggcga cggcacggtc ggcgttttcg gcggacacac
    23581 cgagttcgcg gacccgcgcc gcttctggga tttccgctcg ctcgggcacg gtgatatcaa
    23641 cttcgaggac atcatcgtgg cgctgaacga catcggctat cagggtccgc tctcggtcga
    23701 gtgggaggat tcccgcatgg accgctgcca cggtgcccag gagtcctgcg actttgtcaa
    23761 aggcgtggac ttcccgccca gcgcgatcgc gttcgacgcg caattcgacc gctgaaccta
    23821 aaaaaagagc agttgaaaat tcaactgctc ttttttatta gaatagcggc gggtcaacga
    23881 tctcgtcaaa gggcttcggt tcgatccggc gccagcggca cgcttcctcc ggagggcctt
    23941 cctcttgctc gaaccggtgc cgccagcgtt tctggaagag catcccgttt cgcaccaccg
    24001 ccgccgcatt gcgcaccgtg ggcgtgcctt gcgtggtgac gctctcgaaa tggtacatct
    24061 ccgccgcagg ctccacaaag gcgcgatgcc cggcctcgcg caaccggtag accagatcga
    24121 aatcctcgaa ctgcacggga tggaacgcct cgtcgaagcc gccgtgttcg cggagcagcg
    24181 ccgccgggat catcaggcag gcgctgatca ggcactgcac ttcacgccgc accgtgtaac
    24241 gcg
//
LOCUS       DAOSLE010000021        24183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2317_length_24183_cov_7.014009, whole genome shotgun sequence.
ACCESSION   DAOSLE010000021 DAOSLE010000000
VERSION     DAOSLE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 24183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24183
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2317_length_24183_cov_7.014009"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK576_02875"
     CDS             <1..263
                     /locus_tag="PK576_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60564.1"
                     /translation="AIGIGIDCFTTGHFLGFQGFWVSGFRGFKVSGFQGFWFQGFIQI
                     GVGVAIGIGVAIGIGIDCFTTEHHRLTASPPHSLTASQSHSL"
     gene            779..1222
                     /locus_tag="PK576_02880"
     CDS             779..1222
                     /locus_tag="PK576_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE60565.1"
                     /translation="MNGSINQLLMQKGCDIITIAPTATVYEAIALMNEKNIGSLLILN
                     ANGKFAGIFAERDVFRKVILAGKDPKEVQVKAVMTKKMVYVAPESTVDECMALMTEKR
                     IRHIPVLDDGQKVLGIVSIGDLVKFVASEQDALIKNLERYIEGSL"
     gene            1320..1877
                     /locus_tag="PK576_02885"
     CDS             1320..1877
                     /locus_tag="PK576_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE60566.1"
                     /translation="MSDISSAPAAPCGCACTFDESDQALVAKLHEVIADYRDKPGGLI
                     PALQTAQSLFGYLPEPAIRLIADGFGKTVSEVAGVVTFYSFFTLHPRGKHLVRVCLGT
                     ACYVRGGKEVLDAFRKKLGIDVGETTPDRQFSLEVGRCFGACGMAPVVMVNDTVYQRV
                     KPARVGELIGQIISVSAAGGEKEHA"
     gene            1874..5032
                     /gene="nuoF"
                     /locus_tag="PK576_02890"
     CDS             1874..5032
                     /gene="nuoF"
                     /locus_tag="PK576_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE60567.1"
                     /translation="MKSPTLKKLRDVSELDAWRAKLQAAAGATGADAAPRQILICFGG
                     SCLASGAKEVRDALAAVLKREGLAEQVALVGTGCMGPCVIGPVLMITEDRTFYQGVKP
                     EDAEEIVTEHLKAGRRVERLLVSDPSGQTPAPCRESIEFFKRQTQIVLRNCGWVDPGR
                     IEDTIARDGYAALAKILSGMKPDEVIAEMKASGLRGRGGAGFPTWLKWHLATQTPEGE
                     RYVLCNADEGDPGAYMDRSVLEGDPHSVIEGMAIAAYAIGARQGYVYVRAEYPLAVER
                     LTAAIEQAHACGLLGEKILGSTFSFDLEIRMGSGAFVCGEETALIASIEGRRGEPRPR
                     PPFPAQKGLWGRPTVLNNVETYANVAAILLRGGAWYAGIGTPKSKGTKVFALAGAVRN
                     TGLVEVPIGTPLGEVVYDIGGGIRNNRHFKAAQIGGPSGGCIPRENLNVPLDFETLAE
                     LGAIMGSGGLIVMDEDTCMVDVARFFIEFVQEESCGKCAPCRVGTRRMLEILDRICSG
                     QGEEGDIERLIELGEFIRSASLCGLGQTAPNPVLSTIRHFRHEYEEHIRDRHCRAGVC
                     AGLVRAPCLSACPAGVDVPGFVSLVGDGRYAEALRLHREKNPFASVCARVCFHTCEEK
                     CRRASLDEPVAIRGVKRFMVDQEVIIQVPEVRENAVNAQRKIAIVGAGPAGLSCAYFL
                     ARMGYKPTVFESEPRPGGMLVQAIPAYRLPREELAREVRMIERLGVSIETERKLGRDF
                     TLQDLREQGFEAVFLAMGAALGVTLDLPGADAQGVTEAMRFLRQYNIRGSVPVGKRVA
                     VIGGGNAAIDAARTAIRLGAESVTVLYRRTREEMPAYAEEIEEALQEGVVLRTLIHPE
                     AFEVKNGKVTGVRCHPMRLGEFDRSGRRRPEDALDAEGEVIPADQVIAAIGQALDAEA
                     LAGAVSVERNLRGWIAADPRTGATSVPWLFAGGDAVTGPSSVVEAIAAGERTAVAIDH
                     MLTGEEHAFWRGYCDAGANFDPNADPVPYAREKLPTIALEKRRQNFTEVEQPWNEATA
                     LRQAKRCLRCDYGKQPCGIENA"
     gene            5046..6815
                     /locus_tag="PK576_02895"
     CDS             5046..6815
                     /locus_tag="PK576_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOE60568.1"
                     /translation="MLNVTINLQTIQVPEGTTLLQAAGMIGVRIPTLCNLEHREPIGA
                     CRVCLVEVEGARTLMAACSTPVSEGMKVRTHSARARAARRQVVELLLSEHDGDCPTCD
                     RADDCELRALATELGVAVVPFQGVRAKPHRDVSTPALVRDNGKCIKCRRCVSICSEVQ
                     GVGALFPQGRGFQTVIGPAFTRDLDSVACVQCGQCAAVCPVGAIVEKSSIPEVWKALE
                     DPTKHVIVQTAPAIRAALGECFGYEPGTRVTGKMAAALRRLGFDGVFDTNFTADLTIM
                     EEGTELLTRLKKALVDKEPVALPMFTSCSPGWINFAEFYFPQFLPNISTCKSPQQMFG
                     AVAKSFYAEKIGKKPEEIVVVSVMPCTAKKFEAQRPEMSSSGVLDVDYVLTTRELARM
                     IKEAGIEFTSLPDEAMDAPLGLSSGAADIFANTGGVMEAALRTAYEIVTGRELPVENL
                     HIQAIAGLDGVKEAALTITGTVKEWSFLEGVELKVAVSHGLSNTRRLLEHIKAHGSPY
                     HFIEVMTCPGGCIGGGGQPRFTDDGVRLKRMAAIYSEDESKSLRKSHENPDVQKLYKE
                     YLGKPLGEKSHHLLHTHYASKAR"
     gene            7437..8258
                     /locus_tag="PK576_02900"
     CDS             7437..8258
                     /locus_tag="PK576_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE60569.1"
                     /translation="MEVTFLGTGTSVGVPMIGCRCAVCTSADPRNTRRRTSLYVRTPG
                     AALVIDTPPDFRQQALDFGVDRVDAVVFTHAHADHIFGFDDIRRFNTLQQKVIPAYAD
                     TETLADVRRVFSYIGDRPSPLGLYRPLVEFIEVHGPFTVGDVQLTPLDVCHGNKMTGY
                     LLEREGCRIGYVPDCHTMPDATVRRLRGVDVMILDALRYKPHPSHICVEESLRLLSEI
                     DAGRSYLIHLCHDVDHAELEARLPEGVQVSFDGLRITVDETAAARPLPVRERSAG"
     gene            8291..9100
                     /locus_tag="PK576_02905"
     CDS             8291..9100
                     /locus_tag="PK576_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60570.1"
                     /translation="MAGGVMYAAAVAAQQVEVRLTLAYNGFVIGEPVLVEVELLNATR
                     NPVVVGEKGAQSALFMDVSFGDAYNLLAPVNPEPLAGAFTLNPGQTFRRKLELDKWYP
                     LVREGRYMTQLVLAHEGMRYESVKKSFDVVPGIPVCEGVQMFVNRQKLKRHFKLVYWH
                     RNQTDRLFLRTEDEPGALIWDAIDLGILLRGTTPKLDISPQGEVTVVHRSTQDAFLRT
                     VLWSLPDSLEIVERNSLMDPEISASQRVKSLYGEMAEGQTGEEKKAWWKFW"
     gene            9132..10229
                     /gene="tsaD"
                     /locus_tag="PK576_02910"
     CDS             9132..10229
                     /gene="tsaD"
                     /locus_tag="PK576_02910"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE60571.1"
                     /translation="MVVLGIETSCDETAAAVVADGRRVLSNVVYSQVPLHQPYGGVVP
                     EIASRSHVEKLPGVVAEAVRAAFAPESPAGSAPPPAAWREVDAVAVTYGPGLASSLIV
                     GLSAAKGLALRLERPLIGMNHIEAHIHSVFLNPEAPDPREAAPLLALVVSGGHSCWIE
                     TDRPGNYRMIGQTLDDAAGEAFDKAAKLLGLGYPGGPEIDRLARQAARVDAVAFPKGS
                     PRPGNPALAGLRAELCVSFSGLKTALLYHLRSHPPQDQNEVAEVAAAYQEAIVTALAE
                     RSERALRGGRYAAFAVGGGVSLNRRLRERLTEVARAAGVRLLLAEPRFCGDNAAMIAG
                     LAGAGGGVRGPAAQELDASPALAAGEEGTAW"
     gene            10223..11269
                     /locus_tag="PK576_02915"
     CDS             10223..11269
                     /locus_tag="PK576_02915"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE60572.1"
                     /translation="MVRVTRRVLLSGVAVAWLLCSGVLAADPDPLVVASWNVENLFDT
                     EDDPANKGDDGFTPRGWMRWTAARYRLKLEHLAEIIAGMRPDVLCLMEVENRRVLEDL
                     VQTLERKHAFSLPVIVHRDAEEKRGIDVALLARAAPVATQWLRTAPGQREVVACDFSF
                     GGRRLTVLVNHWKSQFGKKGESDEIRQREARAVRAFLDERLRAEPAAAILVAGDFNDA
                     PDSPILTQDAGFVMDEARVRGDASGRLLFNLAGSLSESARVTYYYAASKKWYTMDAIS
                     VTRGMLADAVPAAPWRVRKSGYTVFKTAAQQDSQGAPIPFRRVRNKKEGDSFRTGYSD
                     HFPVRVELEPSAAP"
     gene            complement(11297..11827)
                     /locus_tag="PK576_02920"
     CDS             complement(11297..11827)
                     /locus_tag="PK576_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOE60573.1"
                     /translation="MRKGKHPFAEGEPIPGESESEGYAVPDESAFLSAASRARCTPTP
                     REAARLAAVRTLFQRVKPGLPIDSDPPELRQEPTPLGHAIETILQRLELRATPWLDDL
                     AAAWHEILPPQVSRFTRPGKYDAGILFVFVTSSTHLFEIRRNHLQRIEQAVRAFAGDE
                     RQIRQVRLMVDSVPIP"
     gene            11878..13116
                     /locus_tag="PK576_02925"
     CDS             11878..13116
                     /locus_tag="PK576_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE60574.1"
                     /translation="MKKGNMLIAQSGGPSMVINQSLVGAVLAARRSGSGVGKIYGALH
                     GIRGILDGAFIDLRKETTATLEAVAATPSSALGSVRHKPSAEDCLAVFRRLRQRDIRY
                     FFYIGGNDSAETVHILHEEAVKNECDIRCFHIPKTIDNDLCGNDHTPGYGSAARFVAC
                     AVKGDDLDNRALGGVKIDVIMGRNAGFLTAAAALARTEEDDGPHLIYLPERPFSVTRF
                     VRDIKAAMRDYGRCVVAVSEGITDRRGTPLVAAFTREKDSHGNVQLSGTGALGDLLAN
                     VVKTRTGITRVRADTFGYLQRSFPGVVSPTDALEARKVGEEAVLAAVGKNMASGSIAI
                     RRKPGKRYGVRFEVVPLHCVAKVTRRMPDAFISPEGNYVTQAFLEYARPLVGALPPCA
                     RFSQLRRCGGGPTPGKRKKA"
     gene            13121..13585
                     /locus_tag="PK576_02930"
     CDS             13121..13585
                     /locus_tag="PK576_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60575.1"
                     /translation="MMAETGSTTETGGTQETLSRTELQIEQEKLKLERERILLERERL
                     EAVRDRMRAEQSLRTDGKGRLAVPLSTLAMLSIICLLVGGILGAFSTSLHVDRRNTAR
                     LQEVMQTLATASPESIGVVTNADHAASAELPAWLKAMKPKGAHAGISLVVIQ"
     gene            13601..14986
                     /locus_tag="PK576_02935"
     CDS             13601..14986
                     /locus_tag="PK576_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOE60576.1"
                     /translation="MAFFKAYDMRGVFGRDFDLDMVWRIGRWLPSVLGARRMLVGRDA
                     RVSSEPIAEALCRGLTEAGCAVEDIGLATTPMVYFFTAQDGYDASVQITASHNPAHHN
                     GMKVSRAGAVPVGYDTGLAEIKARVASGTLPPPAPVPGRRSDIDVRDRFIAWLRERCP
                     DLTGLKLAVDCSDGMAGLVIREVLGEGPLYLNATPDGRFPNHSPNPLEAESRVQVAEA
                     VRKHGLDVGVIFDGDADRVMFVDETGGFVQPDYLIPVIARTFLQREPGATVLHDVRTS
                     RGVIEALREDGARPVMGRVGHAFAKVRMRETGAVCGGELAGHYYFRDFFCCDSGELAA
                     LMVLGVLAEQRRRGVTFSAHIAPIRRYANSGECNYRVRDPDAAIAAALRAAEAFGTVV
                     GRSEIDGVRLEWTDGWLSLRPSHTEPYLRLIVEAEDGERLARRLDVLETALAPFAEEG
                     SVAVRGADGHA"
     gene            14979..15716
                     /locus_tag="PK576_02940"
     CDS             14979..15716
                     /locus_tag="PK576_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60577.1"
                     /translation="MLEIDHISFSYGRRRVIDDLSFDVAPGEIVALTGANGVGKTTLL
                     QILANLLIQDTGNIRLDGVDPLERPIRYRRGIGYLSEMCPLYDEMTVEAYLTYRVRLK
                     GERTLRIRRRVSEALELCGLDKVGREPVRLLSQGYRKRLSLADALALRPKVLLLDDPL
                     AGLDAQLRRRLGAMLSAISSRSAVVVAGHEIQEMLGWCTRVVLLRTGGSALFYRTGEH
                     SREALTEQVGRQVAADTGEEEACVPST"
     gene            15698..16441
                     /locus_tag="PK576_02945"
     CDS             15698..16441
                     /locus_tag="PK576_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE60578.1"
                     /translation="MRTVHMIWSREVRAFVRAFSCGVSVAAFLAVAGWTFVMLMRRYE
                     GSVLQVQTLWGLSVAPWLPLLSAVVTMRLFAEERASGMIELLLAAPLRERDLVIGKFL
                     SALTIIAVALSVAVLVPALVLPSLCPAVQGLVRASALAATSFLLLAQASAWCAVGTLI
                     SVLFRNPAAAAVCAMVVCGGLPVASYAVVLAWMPSVRSGVAWMPLLAQVYDFSTGQFS
                     TGVLILYGSITLFCLFACSKALACLRLRG"
     gene            16465..17976
                     /locus_tag="PK576_02950"
     CDS             16465..17976
                     /locus_tag="PK576_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOE60579.1"
                     /translation="MARVRINQKRVRNAGRRGLRATSMLAVGLGAVLALLVVILGYRL
                     DLTWEVPVGRVRVVSERTRDVLADTQGTIRILCFMDRRHLLFRPVSRLLQGFRRASRS
                     VAGAEIRIEYVDPRWDLTRAGQLAAWGVPENALVFERQRRRIIVTLDEMMAEPSPLQD
                     GTLPDRRRWQGAGPGVFRGERVCAAAVARLALPYERPVIYWLQGHGEGRFDEYDPLHG
                     FSDIARELKRDGFDVRELALPGLDRIPEDGQVLIIAGARRALAAEEVALMDSFLQRGG
                     RLLYLAAPDANTALEPLLEKWGIRVLPLVAVSPRTLSGTEVVVTDFADHGITRNLRNA
                     SVVFGQARCLEMVADPAFTAGADRPQVTPLAQTGPEGWGESRPDVFPRVFDAQSELRG
                     PVTLAAVSERGGTVARDVAFKPTRLCVIGEANFVMNGTLAAQANANRDLFMNAVTWLA
                     GIDVGTAPSLGGDATLHTGFGRRQWVVFLVWSALAVPAAVLALSFLIWARGRW"
     gene            17984..19834
                     /locus_tag="PK576_02955"
     CDS             17984..19834
                     /locus_tag="PK576_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOE60580.1"
                     /translation="MGNRRVLYFLASCILFCLALLWAIQQRRVRSFSESDKSRARLIE
                     QALDGVERITVEHGDARIGLLRRGAEWELVAPFPARVDQGAVARMLDGFETARVTDAL
                     SFQEVRRRELTLRDFGLAVPRTRVELAGREGVNRVLFGAFTPHGTDVYLLMNGLEQVL
                     AVPSPLYRVLPRTADEVRSRSLVCGNRARIRSLEMRMSGHPFIRLSKEQGTWRLTQPA
                     AAPASDARVEALLDVLYREPVERFVWPTVSNVLDVAGSEADFKLRLELYGLGAESGLQ
                     VQVEEVGGTAAERVVFGRTVEGAAALTYVLLPGGNAVGAVSNAVAQAFRLLPADLRDP
                     RVFAGVRGVVRRLDIYVGDNLFVLSRTNALWRIEAPVSDAADQEVADEAVSRLLRLEA
                     EGGEEAASPEAQAGTAAPSRYAPVSHVVLFGDQGETRFTVTRAEPDAPFVRLAFTNSA
                     ALYRVAASNLPSVFMSQDGLMSLRDKTVLALPETTVRRVTLKRADGSVETAQRDSAAD
                     VWRLADGQTGVLSAERLGRLLKSLTSLRAERIEKIGLTPEEGHVYGLTRPWLEMSVDV
                     DAKDSVRKTLLVGDEADLGSRYAVVRGLDVLFVLGGDAVAELAAHWIEPL"
     gene            19855..21426
                     /locus_tag="PK576_02960"
     CDS             19855..21426
                     /locus_tag="PK576_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE60581.1"
                     /translation="MLETVMEFLNGPLGRECFRLNRVLFLVFLALATLAVWRAPAPRP
                     GKVGFGLLRGLTTAALLAILIYQATWQISGFLKPEFVHFLRRYNKRPGAAAMQVMRGP
                     LLDRRGHVLAAPVPGDVWGRRYPLGEAGVHPLGYYHSQFGITAVERVCDPELSGYSVE
                     RREGFPGATLFAVRAEEGRAVTLTLDARLQARAYDLLAGRKGAVIVMRPRSGDLLALV
                     SSPGFNPGDPGPASRDEENAPVFNRATQGRYPPGSVFKILVAAAALDKGLAPVYACPA
                     MGYIAGPNTPPIRDSEYYAYARRGAVWPGWGRLNLEKALVHSSNVYFAQLGVACGAEV
                     FNAMMARAQINAPLVYLQAASGELRSAAGSAPTVTQPRKLAMLAIGQGDLLVTPLHVA
                     CFTAAVAADGVMPRPRLRTDEAPGRMATLCSPEAAGRLTGMLRAAVRNGTGRAAEIPG
                     LEVCGKTGTAQAAGGDDHAWFTCFAPRRKPRLVVTVLVERGGFGARSALPAARALLEE
                     AVRLGLARPEGEGES"
     gene            21423..22703
                     /locus_tag="PK576_02965"
     CDS             21423..22703
                     /locus_tag="PK576_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOE60582.1"
                     /translation="MSARRTRVVSPVGAVAGLFLLSFLAVLSASLSVLGIKLGNAPVM
                     PWEQLIPLGIYAVGVPAVLVWMRVNGWKGDAGLAGAVFLLCGLGVTVQFRMGSFSRGL
                     ADPMALAPLPLGLLAFAAGVALTAKRRGAWLARTGWAAYLAALGVLAALLLLGRKYRG
                     GLYLAGNINPTELVKPLLVLFLASFLSRRQKAFSETQVGIPVPSLDALLVLIFLWAGP
                     VLLTLAVKDLGLLLLLNAILVVMLFAVARSTGYLALGLAAVTAAGYGVRFLSPHVRAR
                     FDVWLNPFEDPTGKGWQILQALAAMYSGGWWGAGLGSGVPQTVPIVTSDFVYAALAEE
                     IGLAGCALLLAVYAVLFSRGFRCAGAARTPFERLLGVGMTGALAMQTILNVAGVTKAL
                     PMTGITLPLISHGGSSLITTLLMAGLVTGLSDRR"
     gene            complement(22722..23075)
                     /gene="secG"
                     /locus_tag="PK576_02970"
     CDS             complement(22722..23075)
                     /gene="secG"
                     /locus_tag="PK576_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOE60583.1"
                     /translation="MQILIGFLYVIEVFVCFLLAAVILLQKPKEGGLGVSFGGGMGEA
                     LFGAQMGNVLTQATIILGAIFLINTLALSRLTAHTGSSLMEGVKPSAPAAEQALPFTQ
                     QPAAPTAPAAPAPMP"
     gene            complement(23729..24079)
                     /locus_tag="PK576_02975"
     CDS             complement(23729..24079)
                     /locus_tag="PK576_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60584.1"
                     /translation="MVDGVTVSVFGYGHGITGRRVWQEDCGLCHSHNAFLYNAPGEVT
                     NAVLRADNYDYSYDAIGNRVWSAVNTLTNSYTANALNQYTLISNPVNPVNPVQIPPPI
                     GACGPPFGNRWLET"
BASE COUNT         3963 a         6702 c         8635 g         4883 t
ORIGIN      
        1 tcgctatcgg gatcgggatc gattgtttca ccacgggaca ctttcttggg tttcagggtt
       61 tctgggtttc agggtttcgg ggtttcaagg tttcagggtt tcagggtttc tggtttcagg
      121 gtttcatcca aatcggggtc ggggtcgcta tcgggatcgg ggtcgctatc gggatcggga
      181 tcgactgttt caccacggaa caccaccgcc tcaccgcctc accgcctcac agcctcaccg
      241 cctcacagtc tcacagcctt tgagattgcg ggctccgccc gcatctcaat acggtggggt
      301 ggtgacgggg gcgttaaact ttaagccagg attacaggat taacaggatt ctgattgagg
      361 ttatcggtaa gccggtcgtc cggggttccg ggcgggaacg gggtggtgac ggggacgctt
      421 acccgtatgc agtgaagagg gggggaggtg cgcgggcgga tgcccgctgg cgggtgttgg
      481 ggcaggcaga cgctcgtccc tcccgatctg agacatcgct atcggatcga tacgttcaag
      541 gtgacttgca agggtaaatg cacggaggcg gaagagcgga gcgatgattt ttatgcggcg
      601 aggttccaag aagcccttgc cgggtgtcgc aatatcgttt acaatttcca gtacagtgat
      661 ggtggggaat gtgaatggtc tgcacccatc tcatccacct cgctgtattg gaccggcatc
      721 aacgggaaga gtccgggctg acgggacaat gaaacggtaa aacggggaag gatgcgaaat
      781 gaacggatcg atcaaccaac tgcttatgca aaaggggtgc gatatcatca ccatcgcgcc
      841 gacagcgacc gtatatgagg cgatcgcgct gatgaatgag aaaaacatcg gctctctctt
      901 gattctgaat gccaacggca agttcgcggg aattttcgct gaacgcgatg tgttccgaaa
      961 ggtgattctg gccggtaaag acccgaaaga ggtgcaggtc aaggcggtca tgaccaagaa
     1021 gatggtctat gtggcgccgg agagcacggt ggacgagtgt atggcgctga tgactgaaaa
     1081 gcgtatccgc catatcccgg tgttggacga cggccagaag gtgctgggca tcgtctcgat
     1141 cggggatctg gtgaaattcg tggcctccga acaggatgcg ctgatcaaaa atctcgaacg
     1201 gtatatcgaa ggttccctgt aaggacgttt tccgtccgtt ttggagcaga tcgggactgt
     1261 ttcaggcgcg ggcggttttt cggttcaacg ttcccccgca ggggacaacg gaggaaagaa
     1321 tgagtgatat ctcttccgct ccagccgcac cgtgcggctg cgcgtgcaca tttgatgaat
     1381 ccgatcaggc tctggtcgcg aaattacatg aggtgatcgc cgactaccgc gacaagcccg
     1441 gcgggctgat tcccgcgctt cagacagccc aatccctttt cggttatctg cctgagcctg
     1501 ctatccgcct gatcgctgac ggtttcggca aaaccgtcag cgaagtggcc ggtgtggtca
     1561 cgttctattc gttctttacc ctgcatcccc gcggcaagca tctggtgcgc gtctgtctcg
     1621 gtacggcctg ctatgtgcgc ggtggtaaag aggtgttgga cgccttccgt aagaagctcg
     1681 gaatcgacgt gggggagacc actccggacc gtcagttctc tctggaggtg gggcgctgtt
     1741 tcggcgcgtg cggcatggcc ccggtggtca tggtgaacga cacggtgtat cagcgtgtca
     1801 aaccggcccg tgtgggtgaa ctgatcggtc agattatttc ggtatccgcc gcaggcgggg
     1861 aaaaggagca cgcatgaaga gcccgacgct gaaaaaactg cgggacgttt ccgaactcga
     1921 tgcttggcgg gcgaagcttc aggccgcggc gggtgccacg ggcgccgacg ccgcgccgcg
     1981 ccagatcctg atctgctttg gcggcagttg cctcgcctcc ggtgccaaag aggtgcgcga
     2041 cgctctggcc gccgtgttga aacgcgaggg gctcgcggag caggtggcgc tggtcggcac
     2101 cggctgcatg ggcccgtgcg tcatcggtcc cgtgctgatg atcactgagg accgtacttt
     2161 ttatcagggc gtcaaacccg aagacgccga ggagatcgtc accgagcatc tcaaggctgg
     2221 ccgtcgggtc gagcgactct tggtgagcga cccttccggg cagactcccg cgccttgccg
     2281 cgaaagcatc gagtttttca agcgccagac gcagattgtg ttgcgtaact gcgggtgggt
     2341 cgatcccggc cgtatcgaag acacgatcgc gcgtgacggg tatgcggcgc tggccaagat
     2401 cctctccggt atgaagccgg atgaggtgat cgcggaaatg aaggcctccg gattgcgcgg
     2461 ccgtggcggt gccggattcc cgacgtggct gaagtggcac ctcgccaccc agacgcccga
     2521 gggcgaacgc tacgtgctct gcaacgcgga tgagggcgac ccgggcgcgt acatggaccg
     2581 cagcgtgttg gagggcgatc cccacagcgt gatcgagggg atggccatcg ccgcctacgc
     2641 catcggcgcg cggcaggggt atgtctatgt gcgggccgaa taccctcttg ccgtggagcg
     2701 gctcactgcc gccatcgaac aggcgcacgc ctgcggactg ttgggcgaaa agatactcgg
     2761 ttcaacgttt tcgttcgatc tcgaaatccg catgggctcc ggggcgttcg tctgcggcga
     2821 ggagaccgcg ctgatcgcct ccatcgaggg caggcgcggc gagccgcggc cgcggccgcc
     2881 tttcccggcc cagaaggggc tgtgggggcg gcccacggtg ctcaacaatg tcgagaccta
     2941 cgccaacgtg gccgcgatcc tgctgcgggg cggcgcctgg tacgccggga tcggcacgcc
     3001 caagagcaag ggcaccaagg tgttcgcgct ggctggcgcg gtgcgcaaca ccggacttgt
     3061 ggaggtgccc atcggaactc cgttgggcga ggtggtctat gatatcggtg gcggcatccg
     3121 caacaaccgc catttcaagg cagcccagat cggcgggccg tcgggtggtt gcatcccgcg
     3181 cgaaaacctc aatgtgccgc ttgatttcga aaccctcgcc gaactcggcg cgatcatggg
     3241 ttcgggcggt ctgattgtga tggatgagga cacctgcatg gtggatgtcg cgcggttctt
     3301 catcgaattc gtgcaggagg agtcgtgcgg caagtgcgcg ccctgccggg tcggtacacg
     3361 tcgtatgctc gaaatccttg accgcatctg ttccggtcag ggcgaggaag gggatatcga
     3421 gcggctcatc gagttgggcg agttcatcag gtccgcctcg ttgtgcggac tcggccagac
     3481 cgcgccgaat ccggtgctct ccacgatccg tcacttccgc cacgaatatg aggagcacat
     3541 ccgcgaccgg cattgccgcg caggggtctg tgccggtctg gtgcgcgcgc cctgcctgag
     3601 cgcctgtccc gcaggggtcg atgtgccagg cttcgtttcg ctggtcggcg acggacggta
     3661 tgccgaggcg ttgcggctgc accgcgagaa aaaccccttc gcatcggttt gcgcgcgcgt
     3721 ctgcttccat acctgcgaag agaaatgtcg gcgcgcctcg ctggacgaac cggtcgctat
     3781 ccgaggcgtc aagcgtttca tggtggacca ggaggtcatc atccaagtgc cggaagtgcg
     3841 cgagaacgcc gtaaatgcgc agcgcaagat cgcgatagtc ggcgcggggc cggcgggtct
     3901 ctcatgcgcc tatttcttgg cgcggatggg gtacaaacca acggttttcg agtctgaacc
     3961 gcggccgggt ggtatgctgg tgcaggccat tcccgcctac cgcctgccgc gtgaagagtt
     4021 ggcgcgcgaa gtgcgcatga ttgaacgcct tggtgtgagc atcgaaaccg aacgcaaact
     4081 gggccgcgat ttcacccttc aggacctgcg cgaacagggg tttgaagcgg tctttctcgc
     4141 gatgggcgcg gcattgggcg taacgcttga cctgcccggc gcggatgcgc agggtgtgac
     4201 cgaagccatg cgttttctgc ggcagtacaa tatccgcggt tcggtgccgg tgggcaaacg
     4261 ggtggcggtg atcggcggcg gcaacgcggc cattgatgcg gcccgcacgg cgatccgcct
     4321 cggggccgag agcgttaccg tgctctaccg gcgcacgcgc gaagagatgc ccgcctacgc
     4381 cgaggagatc gaggaggcgc tgcaggaggg ggtcgttctc cgcacgctga tccatcccga
     4441 ggccttcgag gtcaagaacg gcaaggtgac gggagtgcgt tgccacccca tgcggctcgg
     4501 cgagttcgac cgctcgggcc gtcggcgtcc cgaggatgcg ctggatgccg agggcgaggt
     4561 gatccccgca gatcaggtga tcgcggccat cggtcaggcg ctggatgcgg aggcgctggc
     4621 tggcgcggtt tcggtcgaac ggaatctgcg cggctggatc gccgcggatc cgcgtacagg
     4681 cgccacttcg gtgccctggc tctttgcggg cggggacgcc gtgaccggac cgtcgtctgt
     4741 ggtggaggcg atcgccgcgg gcgaacggac cgcggtggcc atcgaccaca tgctgaccgg
     4801 cgaggagcac gccttctggc gcgggtattg cgatgcaggt gcgaattttg acccgaatgc
     4861 ggaccccgta ccctatgcgc gggagaagtt gccgacgatc gcactggaaa aacggcggca
     4921 gaatttcacg gaggtcgaac agccgtggaa cgaggccacg gcattgcgtc aggccaagcg
     4981 ctgtctgcgg tgtgattacg gcaagcagcc ctgcggaatt gaaaacgcct aatttggaga
     5041 agcccatgtt aaatgtgaca atcaatttgc agacgatcca ggtgccggaa ggcaccactc
     5101 ttcttcaggc ggcgggcatg atcggggtgc gcatcccgac gctctgcaat ctggagcatc
     5161 gcgaacccat cggcgcatgc cgtgtctgcc ttgtggaagt cgagggggcg cgcacactga
     5221 tggcggcgtg ttccacgccg gtttccgaag gcatgaaggt gcggacgcac agtgcgcggg
     5281 cgcgcgctgc gcggcgtcag gtggtggaac tcctgctgtc agagcatgac ggcgactgtc
     5341 ccacgtgcga ccgcgccgac gattgcgaat tacgcgcgct ggcgacggag ttgggcgtgg
     5401 cggtcgtgcc gtttcagggg gtgcgcgcca aaccgcatcg ggacgtttcc acgcctgctt
     5461 tggtgcgcga taacggcaaa tgcatcaagt gtcggcggtg cgtttcaatc tgcagcgagg
     5521 tgcagggggt cggcgcgctc ttcccgcagg gccgcggctt ccagactgtc atcggtccgg
     5581 ccttcacgcg ggatctcgac agcgtggctt gcgtgcagtg cggccagtgt gccgcggtct
     5641 gcccggtggg cgcgattgtc gaaaagagta gcatccccga ggtttggaag gcgctggagg
     5701 atccgacgaa acacgtgatc gtgcagaccg cgccagccat ccgtgccgcg ttgggcgaat
     5761 gtttcggcta cgagcccggt acgcgcgtga ccggcaagat ggccgccgcg ctcagaaggc
     5821 tggggttcga cggcgtgttc gacaccaatt tcacggcgga tctgaccatc atggaagagg
     5881 gcaccgaact gctcacccgc ctgaagaagg cgctggtcga caaggagcct gtggccttgc
     5941 cgatgttcac gagttgctcg ccgggctgga tcaacttcgc ggagttctat ttcccgcagt
     6001 ttctgccgaa catctccacc tgcaaatcgc cgcagcagat gtttggcgcg gtggccaaga
     6061 gtttttatgc cgagaagatc ggcaaaaagc ctgaagagat tgtggtggtc tcggtcatgc
     6121 cttgcaccgc caaaaagttt gaggcccagc gtccggaaat gtcttccagc ggtgtgcttg
     6181 acgtggatta cgttctcacc acgcgtgagc ttgcgcggat gattaaggag gcggggatcg
     6241 agttcacgtc gctgccggat gaggcgatgg acgcgccgct ggggctctcc tcgggcgcgg
     6301 cggacatttt cgcgaacacc ggcggggtca tggaggccgc gctgagaacc gcgtacgaga
     6361 tcgtgaccgg ccgcgagctg ccggtcgaaa acctgcatat ccaagccatc gcgggtctcg
     6421 atggggtgaa ggaggcggca ctgaccatta ccggcacggt gaaggagtgg tcgtttttgg
     6481 agggggtcga gctgaaggtg gcggtttcgc acggcctcag caacacgcgg cgtctgctgg
     6541 agcacatcaa ggcccacggc tcgccctacc acttcatcga ggtcatgacc tgccccggcg
     6601 gttgcatcgg cggcggcgga caaccgcgct ttacggatga cggcgtgcgg ctgaaacgta
     6661 tggccgcgat ttacagcgag gacgagagca agagcctgcg caagtcccat gagaatccgg
     6721 acgtgcagaa gctctacaag gagtatctgg gcaagccgct cggtgaaaaa tcgcaccatc
     6781 tgctgcacac acactacgca tccaaggcgc ggtgaggtca gattgaggaa acgctcaaca
     6841 ctggcataac cacccccggt cctgttgata aggttggcct cacggggcct tgaggggccc
     6901 ccggccaact taccagcagg cgtgtggggg tggttataca agatgcccct gcttttcagg
     6961 ggtttctggt ttcagggttt catggtttca ggtttcaggg tttcatccaa atcggggtcg
     7021 ggatcgctat cgggatcggg atcgattgct tcaccacggg acactttgtt gggtttcatg
     7081 gtttcagggt ttcaaggttt cagggtttca aggtttcagg gtttcatcca aatcgggatc
     7141 gggatcgcta tcggggtcgc tatcgggatc gggatcgatt gcttcaccac gggacacttt
     7201 gttgggtttc tgggtttcat ggtttctggt ttcatgggtt tcaaggtttc agggtttctg
     7261 ggtttcatgg tttctggttt caaggtttca gggtttcagg gtttcagggt ttctggtttc
     7321 atggtttcat gccacgcatg cggcgtaaat tgtggccggg ggaataagtc ttccgtcaga
     7381 aaccaacatc tgaagtggcg gaggcccggg cttgtgctat actgaccgaa atgtccatgg
     7441 aagtgacttt tttgggtacc ggaacgtccg tcggggtgcc gatgatcgga tgtcgttgcg
     7501 cggtctgcac ttcggcagac ccccgcaaca cgcgtcggcg gactagcctg tacgtgcgga
     7561 cgcccggagc cgccctggtg atcgacacgc cgccggattt ccgtcagcag gctctggact
     7621 tcggtgtgga tcgggtggat gcggtggtgt ttacccacgc ccacgcggat cacattttcg
     7681 gtttcgatga catccggcgt ttcaatacgc tgcagcagaa ggtgattccg gcctatgcgg
     7741 atacggagac gcttgcggat gtgcggcgcg tcttcagcta tatcggcgac cgcccgtcgc
     7801 cgttaggact ctaccggccg ctggtcgagt tcatcgaggt ccatggaccg ttcacggtgg
     7861 gcgatgtgca gttgacgccg ttggatgtgt gccacggcaa caagatgacg gggtatctgc
     7921 tggaaaggga ggggtgccgg atcgggtatg tgcccgactg tcacacgatg cccgatgcga
     7981 ccgtccggag gttgcggggc gtggatgtga tgattctgga tgcgctgcgc tataaaccgc
     8041 atccgtcgca tatctgcgtg gaggagagtt tacggctcct gtccgagatc gatgcgggcc
     8101 gctcgtatct gatccacctc tgccatgatg tggatcatgc ggagttggag gcgcgtctgc
     8161 cggagggcgt gcaggtgagt tttgacggat tgcggatcac ggtggacgag actgcggcgg
     8221 cgcggccgct tcccgtccgc gaaaggagtg cgggatgacg gggttctcaa gaatacggag
     8281 gtgcgcgtgt ttggcggggg gcgtaatgta tgcggcggcg gtcgcggcgc agcaggtgga
     8341 ggtcagactg acgctcgcct acaacgggtt tgtcatcggc gaaccggtgc tggtggaggt
     8401 ggaactgctg aacgccaccc gcaatccggt cgtggtgggg gaaaagggtg cccagagcgc
     8461 gttgttcatg gacgtgtctt tcggtgacgc ctacaatctg ctcgccccgg tgaatccgga
     8521 accgctggcc ggggcattca cgctcaatcc cggtcagacg ttccgccgga agctcgaact
     8581 ggacaagtgg tatccgctcg ttcgcgaggg acgctacatg acgcagctcg tgctggcgca
     8641 tgagggcatg cggtatgagt ccgttaaaaa gagcttcgat gtggtgcccg gcattccagt
     8701 gtgcgagggg gtgcagatgt ttgtcaaccg gcagaagttg aaacgccact tcaaactggt
     8761 ctactggcat cgtaatcaga ccgaccggtt gtttttgcgg accgaggatg agcctggggc
     8821 gctaatctgg gacgcgatcg atctcggtat cttgctgcgc ggcactacgc ccaaactcga
     8881 tatctcgccc cagggcgagg tgaccgttgt ccatcgctcg acgcaggatg cgtttctccg
     8941 cacggtgttg tggtcgctgc ccgacagtct tgagatcgtg gagcgcaact cgctgatgga
     9001 tccggaaatt tcggcgtcgc agcgggtcaa gtcgctgtac ggggagatgg ccgaaggtca
     9061 gaccggggaa gagaagaagg cgtggtggaa gttctggtga gccgcgggag ggcggtgggg
     9121 gtgcccggcg catggtggtt ttagggatcg agacgtcgtg cgacgaaacc gcggccgcag
     9181 tggttgctga cggtcggcgc gtgctgtcga acgtggtgta cagccaagtg ccgctgcatc
     9241 aaccctatgg cggtgtggtg ccggagatcg cttcgcgcag tcatgtcgag aagctgcccg
     9301 gtgtggttgc cgaggcggta cgcgcggcct tcgcgccgga gtctcctgcg ggatccgcgc
     9361 cgccacctgc ggcgtggcgg gaggtggatg cggttgcggt gacctacggg ccgggattgg
     9421 cgagttcgct gatcgtgggt ctgtcggctg ccaaagggct cgccttgcgg ctggaacggc
     9481 cgttgatcgg gatgaaccat atcgaggcgc acatccactc ggtctttctg aacccggagg
     9541 cacccgatcc gcgcgaagcc gcgccgctgc tggcgttggt cgtatccggc gggcactcat
     9601 gctggatcga gacggatcgg cccgggaact accggatgat cggacagaca ctggatgatg
     9661 cggcgggtga ggcgtttgac aaggcggcca aactgttggg gttggggtat cccggcggtc
     9721 cggagatcga ccgtctggcg cgtcaggcgg cgcgtgtgga cgcggtggcc tttccgaaag
     9781 ggagtccgcg gccgggcaat cccgcgctgg cgggattgcg cgccgaactg tgcgtgagtt
     9841 tcagcggact caaaacggcg ctcttgtatc atctgcgctc ccatccgccg caggatcaga
     9901 acgaagtggc cgaggtggcg gccgcctatc aggaggcgat cgtcaccgca cttgccgaac
     9961 ggtcggaacg ggcgttgcgg ggcgggcgat acgccgcgtt cgcggtgggt ggcggcgtgt
    10021 cgctcaaccg gaggttacgc gagcgtctga cagaggtggc ccgcgcggcg ggcgtgcgtt
    10081 tgctgttggc tgagccgcgt ttctgtgggg ataacgccgc gatgatcgcg ggactcgcgg
    10141 gggcgggcgg tggtgtgcgc ggacctgccg cgcaggaact ggatgcgtcg ccggcacttg
    10201 cggccgggga ggagggaacg gcatggtgag ggtgacgcgg cgcgttttgt tgtccggagt
    10261 cgctgtggcg tggttgctgt gctccggtgt ccttgcggca gatcccgatc cgctggtggt
    10321 ggccagttgg aatgtcgaga acctcttcga cacggaggat gacccggcca acaagggaga
    10381 cgacggcttc acgccgcggg gctggatgcg ctggactgcg gcgcgttacc ggttgaagct
    10441 cgagcatctc gcggagatca tcgcaggcat gcggccggat gtgctctgcc tgatggaagt
    10501 cgagaaccgc cgtgtgctcg aggatctggt gcagacgctg gagcgcaaac acgcattcag
    10561 ccttccggtg atcgtgcatc gggacgcaga ggagaaacgc ggcatcgatg tggcgctgct
    10621 ggcacgtgcc gcgcctgttg ccacgcagtg gctgcgtacc gcgccgggac agcgcgaggt
    10681 ggtggcctgc gatttttcct ttggcgggcg gcggctgacg gtgttggtga accattggaa
    10741 atcgcagttc ggaaaaaagg gggagagtga cgagatccga cagcgggaag cgcgtgcggt
    10801 acgggccttt ctggacgagc ggttgcgcgc ggaaccggcc gccgcgattc tggtggcggg
    10861 cgactttaat gacgcgccgg actcgccgat cctgacccag gatgccggat tcgtcatgga
    10921 tgaggcgcgg gtgcgcggcg acgcatccgg tcggctgctg ttcaatctgg cgggttcgct
    10981 ttccgagtcg gcgcgcgtga cctactatta tgcggccagc aagaagtggt acacgatgga
    11041 tgccatcagc gtgacgcgcg gcatgctcgc cgatgccgtg cctgcggcgc cctggcgcgt
    11101 gcggaaaagc gggtatacgg ttttcaaaac tgcggcgcag caggactcgc agggcgcgcc
    11161 gatccccttc cggcgtgtgc gaaacaaaaa ggagggcgat tcctttcgca ccggctactc
    11221 ggatcacttc ccggtgcgcg ttgaattgga gccgtccgca gcgccgtaag ggtgcggccg
    11281 gggcgcggtc cgggtgtcac ggtatgggca cggagtcgac catcaggcgc acctggcgga
    11341 tttggcgctc gtccccggcg aatgcgcgca ccgcctgttc gatccgttga aggtggttcc
    11401 ggcggatctc aaacaggtgg gtggaagagg tcacgaacac gaacaggatg cccgcgtcgt
    11461 atttgccggg acgggtgaaa cgcgacacct gcggcggcag aatctcatgc caggccgcag
    11521 ccaggtcatc gagccaaggg gtggcgcgga gttcgagacg ctgcaggatg gtttcaatgg
    11581 cgtggccgag cggcgtcggt tcctgacgca gttccggcgg gtcgctgtcg atcggcaggc
    11641 ccggtttgac gcgctggaac agcgtgcgga ccgcggcgag gcgggctgct tcgcgtggcg
    11701 tgggggtgca ccgcgcacgg ctggcggcgg agaggaaagc ggactcgtcc ggaacggcgt
    11761 agccctccga ctcgctctcc cccgggatcg gttccccttc cgcaaacgga tgtttgcctt
    11821 tacgcatgcg gacagggtat catatcggaa ccaatcaggt ggaaagaaaa tgacgacatg
    11881 aaaaaaggaa acatgctgat cgcccaaagc ggcggtccct cgatggtcat caaccaaagt
    11941 ctggtcggcg ccgtgctggc cgctcggcgc agcggctccg gggtcggtaa gatctatggg
    12001 gcgctgcacg ggatccgcgg gatcctcgac ggggcgttca tcgatctgcg caaggagacg
    12061 acggctacgc ttgaggcggt cgccgctacg ccttcgtcgg ctctgggttc ggtgcgtcac
    12121 aagccttcgg cagaggattg cctggcggtg ttccgccggt tgcggcagcg cgacatccgt
    12181 tattttttct acatcggcgg caacgactcc gccgaaacgg tgcacattct tcatgaggag
    12241 gcggtgaaga atgagtgtga tatccgttgt ttccacattc ccaagacgat cgacaacgat
    12301 ttgtgcggga atgaccatac gccggggtac ggcagcgcgg cccgtttcgt ggcgtgtgcc
    12361 gtgaagggag acgatctcga caaccgcgcg ctgggggggg tcaagatcga tgtgatcatg
    12421 gggcgcaacg cgggtttttt gaccgctgcc gcggcgttgg cccgtacgga agaggatgac
    12481 ggcccgcacc tgatctatct gcccgagcgg ccgttcagcg tcacgcgttt cgtgcgtgac
    12541 atcaaggcgg cgatgcgcga ctacggacgg tgtgtggtgg cggtctcgga agggatcacg
    12601 gaccggcgcg gcacgccgct ggtggcggcg ttcacgcgcg agaaggattc gcacggcaac
    12661 gtgcagttga gcggcaccgg tgcgctgggc gatctgctgg ccaatgtggt caaaacgcgt
    12721 acgggcatca cgcgcgtgcg ggccgacacg tttggatatc tccagcgctc ctttccgggg
    12781 gtggtgtcgc cgacggatgc gctggaggcg cggaaggtgg gtgaggaggc ggtccttgcg
    12841 gcggtcggga agaatatggc gagcggatcc atcgcgatcc ggcgaaaacc gggcaagcgg
    12901 tacggggtgc gattcgaggt ggtgccgctg cattgcgtgg ccaaagtgac gcgccgcatg
    12961 cccgatgcgt ttatcagtcc ggaaggcaat tacgtgacgc aggcgtttct cgaatatgcc
    13021 cggcctctgg tgggcgcgct gccgccttgc gcgagattct ctcagttgcg gcggtgcggc
    13081 ggcggtccga ccccgggcaa acggaagaag gcgtgatgat atgatggcag agacgggctc
    13141 aacaacagag acgggcggga cgcaagagac gctctccaga accgagttgc agattgaaca
    13201 ggagaaactg aaactcgagc gggagcggat cctgttggaa cgcgagcgtc ttgaggcggt
    13261 tcgcgaccgg atgcgggccg agcagagttt gcgcacggat ggcaaggggc gcctcgcagt
    13321 gccgctctcc acgctggcga tgctctcgat catctgtctg ttggtcggcg gcattctggg
    13381 ggcgttctcg acctccctgc atgtggaccg gcgcaataca gcccggctgc aagaggtgat
    13441 gcagacgttg gccaccgctt cgccggaatc gatcggcgtg gtgaccaatg cagatcacgc
    13501 ggcttcagcc gaattgcccg catggcttaa ggcgatgaag cccaaaggcg cgcatgcggg
    13561 catttcgctg gtggtgatcc aatagacggg aaagggcagg atggcatttt tcaaggctta
    13621 tgatatgcgg ggcgtgttcg gtcgcgattt cgatctcgac atggtctggc gcatcgggcg
    13681 ctggctaccg tcggtgctcg gtgcgcgacg catgctcgtg gggcgcgacg cccgtgtctc
    13741 ctctgagccg atcgccgagg cgttgtgccg cggactcacg gaagcggggt gtgcggtgga
    13801 ggacatcgga ctggcaacca cgccgatggt ctattttttc acggcgcaag acggatatga
    13861 cgcttcggtg cagatcacgg cctcccataa cccggcgcac cacaacggca tgaaggtgtc
    13921 gcgtgcgggt gccgtgccgg tggggtacga taccggactt gcggagatca aggcgcgcgt
    13981 ggcctcgggc acgctgccac cgcccgcgcc ggttccggga cgccgcagcg atattgacgt
    14041 gcgcgaccgt ttcatcgctt ggttgcgcga acggtgtccc gacctcacgg gactcaagct
    14101 ggcggtcgac tgttccgacg ggatggcggg actcgtgatc cgcgaggtgc tgggagaggg
    14161 gccgctgtat ctgaacgcga cgcccgacgg gcggttcccg aatcattcgc ccaacccgct
    14221 ggaggcggaa agccgcgtac aagtggcgga ggctgtgcgg aagcacgggt tggatgtcgg
    14281 cgtgattttc gacggcgatg ccgaccgggt gatgtttgtg gacgagaccg gcgggttcgt
    14341 gcaaccggat tatctgattc cggtgattgc gcgcaccttt ctccagcgtg agccgggggc
    14401 cacggtgctt catgacgtgc gcacgtcacg cggggtgatc gaggccctgc gcgaggacgg
    14461 cgcgcggccc gtgatgggcc gtgttggcca tgcgttcgcc aaagtccgga tgcgcgagac
    14521 cggggctgtc tgcggcggcg aattggcggg gcactactat ttccgcgatt ttttctgttg
    14581 cgattccggc gaactggcgg ccttgatggt gctgggcgtg ttggcggagc agcggcgtcg
    14641 gggcgtgacc ttcagcgcgc acatcgcgcc gatccggcgg tatgcgaatt cgggcgagtg
    14701 caactatcgt gtccgcgatc cggatgccgc gatcgcggcg gcgttacgcg cggccgaggc
    14761 gttcgggacg gtggtggggc gtagcgagat cgacggggtg cggctggaat ggacggacgg
    14821 atggctgagc cttcgcccgt cccataccga gccctatctg cggctgattg tcgaggcgga
    14881 ggacggtgaa cggctggcgc ggcgcttgga cgtgctggaa accgcgttgg ccccgtttgc
    14941 ggaggaaggg agcgtcgcgg tgcgcggcgc ggacggacat gcttgagatt gaccacatct
    15001 cgttttccta tggacggcgg cgggtgatcg acgacctttc cttcgatgtc gcgccagggg
    15061 agatcgtggc cctcaccggt gccaacggcg tgggcaagac gacgctgctc cagattctgg
    15121 cgaacctgct gatacaggat accggaaaca tacggttgga cggggtggat ccgttggagc
    15181 gccccatccg gtaccgccgc gggatcggtt acctgtccga aatgtgtccg ctttatgatg
    15241 aaatgacggt ggaggcctat ctgacgtacc gcgtgcggct caaaggcgag cgcaccctgc
    15301 gcatacgcag gcgggttagc gaggcgctgg agttgtgcgg actcgataag gttggccgcg
    15361 agcctgtccg gctcctttcg caggggtatc gcaaacggct gagtctggcg gacgcgctgg
    15421 cgctccgccc gaaagtgctg ttgctggatg atccgctcgc cggactcgac gcccagttgc
    15481 gcaggcgact cggcgcgatg ctgagcgcga tctcatcgcg ttccgccgtg gtggtggcgg
    15541 gacatgagat tcaagagatg ctcggctggt gtacgcgggt cgtgttgctg cggacgggcg
    15601 gttcggcgct gttctaccgc acgggtgagc attcgcgtga agcattgacg gagcaggtcg
    15661 ggcggcaggt ggcggccgac accggggagg aggaggcatg cgtaccgtcc acatgatctg
    15721 gtcgcgggag gtccgggcct ttgtccgcgc gttttcgtgc ggggtgtcgg tggcggcgtt
    15781 tctggctgtc gcgggctgga cctttgttat gctgatgcgg cgttacgagg gcagcgtgtt
    15841 gcaggtgcag acgctctggg gtctctcggt ggcgccgtgg ctgccgcttc tgagcgctgt
    15901 ggtgacgatg cgtctctttg ccgaagagcg cgccagcggc atgatcgagc tgttgctggc
    15961 cgcgccgttg cgcgaacgcg atctggtgat cggcaaattc ctgtcggccc tgacgatcat
    16021 cgccgtggca ctgtctgtgg ccgtgctggt tccggccttg gtgttgccgt cgctctgtcc
    16081 ggcggttcag gggttggtgc gagcgagcgc gctggccgca acgtccttcc tgctcttggc
    16141 gcaggcatcc gcctggtgtg ccgttggtac gctgatttcg gtgcttttcc gcaatccggc
    16201 cgcagccgcg gtgtgcgcga tggtcgtgtg cggcggcctg ccggtggcga gctacgcggt
    16261 ggttttggcc tggatgcctt cggtgcggtc cggggtggct tggatgccgc tgctggcgca
    16321 ggtctatgat ttttcgaccg ggcagttctc caccggcgtg ctgatccttt acggatcgat
    16381 cacgctattc tgcctgttcg cgtgttcaaa ggcgttggca tgcctgaggt tgcggggttg
    16441 attccgggaa gacggaggaa aaacgtggcg agggtgcgca tcaatcagaa acgggtccgg
    16501 aacgccggac ggcgtgggct gcgggccacg tcgatgctgg cggtcggtct gggcgcggtt
    16561 ttggccctgc tggtcgtgat cctggggtac cgtcttgact tgacctggga ggtgccggtt
    16621 gggcgggtgc gtgtggtttc ggagcgtacg cgcgatgtgc tggccgacac gcagggaacc
    16681 atccgcatcc tctgtttcat ggaccggcgg catctgttgt tccggccggt gtcgcgtctg
    16741 ctgcaaggct tccgccgcgc ttcgcgcagt gtcgccggag cggaaatccg catcgaatat
    16801 gtggatccgc gatgggatct gacgcgggcc ggacagttgg cggcctgggg cgtgccggag
    16861 aatgcgctgg tgttcgaacg ccagcggcgc cggatcattg tgaccttgga cgagatgatg
    16921 gccgagccct cgccattgca ggacggaact cttcccgacc ggcggcgttg gcagggggcg
    16981 gggccgggcg tgttccgcgg agaacgcgtg tgcgcggccg ctgtggcgcg acttgcgttg
    17041 ccgtacgaaa ggccggtgat ctattggctg cagggccacg gcgagggacg tttcgacgag
    17101 tacgatccgc tgcacggatt ttcggatatc gcgcgtgagt tgaagcgtga cggctttgac
    17161 gtgcgcgaac tcgcgttgcc cggacttgac cggatccccg aggacggaca ggtgctgatc
    17221 attgccggtg cccgccgtgc gctggctgcg gaggaggtcg cgttgatgga ctcctttctg
    17281 cagcggggcg gccggctgct ctatctggcg gcaccggacg ccaacacggc cttggagccg
    17341 ttgctcgaga aatggggcat ccgggtcttg ccgctggtgg cggtcagtcc gcggaccctc
    17401 tcgggcaccg aggtggtcgt cacggatttt gcggatcacg ggatcacgcg taacctgcgg
    17461 aacgcgtcgg tggttttcgg acaggcacgt tgcctcgaga tggtcgccga tccggcgttc
    17521 acggcaggcg cggaccgtcc gcaggtgacg ccgctggcgc agaccggacc ggaaggatgg
    17581 ggggagtcgc gtccggatgt gtttccgcgc gtgtttgacg cccagagcga attgcgcggg
    17641 ccggtcacgc tggctgcggt gtcggagcgg ggcggcacgg tcgcgcgcga tgtggcgttc
    17701 aagcccacgc gtctgtgtgt gatcggcgag gcgaattttg tgatgaacgg gacgctggcg
    17761 gcgcaggcca acgccaaccg tgacctgttc atgaacgctg tgacctggct ggcgggtatt
    17821 gatgtcggta ccgcgccttc gctcggcggc gatgcgacac tccacaccgg attcggtcgg
    17881 cggcagtggg tggtgtttct ggtgtggtcg gctttggcgg tgccggctgc ggtgctggcg
    17941 ctctcgtttc tgatctgggc gcgcggacgg tggtgaggga gttatgggga atcgtcgggt
    18001 tttgtatttt ttggcctcct gcattctgtt ctgcctggca ttactgtggg ctatccagca
    18061 gcgccgggtg cgctcttttt ccgagtcgga caagagccgc gcccgattga tcgaacaggc
    18121 gctggacggg gtggaacgga tcacggtgga gcacggcgat gcccgcatcg gtctgttgcg
    18181 gcggggtgcc gaatgggagt tggtcgcacc gtttccggcg cgggtcgatc agggtgcggt
    18241 ggcgcggatg ctggacgggt ttgaaacggc ccgcgtgacc gacgcgctca gttttcagga
    18301 ggtccggcgc cgcgagctta cgttgcgcga tttcggtctc gctgtgccgc gtacgcgggt
    18361 tgaactggct gggcgcgaag gggtcaaccg ggttttattc ggcgcgttca cccctcacgg
    18421 aacggatgtc tacctgttga tgaacggact ggagcaggtg ctggccgtgc cgtcgccgct
    18481 ctaccgggtg ctgccccgca cagcggatga ggtccgctcg cgtagcttgg tgtgcggcaa
    18541 ccgcgcgcgg atccgttcgc tcgaaatgcg gatgtccggc cacccgttca tccggttgtc
    18601 gaaagagcag gggacctggc gtctgacgca acctgcggcc gcgccggcct cggatgcgcg
    18661 ggtcgaggct ctgctggatg tgctgtaccg cgaacccgtg gagcggttcg tctggcccac
    18721 ggtttcgaat gtgctggatg tcgcgggctc ggaggctgat tttaagctgc gtctcgaact
    18781 gtacggactc ggtgcggaaa gcggtttgca ggtgcaggtg gaggaagtcg gtggcacggc
    18841 ggccgaacgt gtggtgttcg gccggacggt ggagggtgcg gcggcgctga cgtatgtgct
    18901 tctgccgggc ggtaatgcgg tcggtgccgt atccaacgcg gttgcgcagg cattccggtt
    18961 gctgcccgca gacctgcgtg acccgcgggt cttcgcgggc gtgcgtggag tcgtcaggcg
    19021 gcttgacatc tatgtgggcg ataacctgtt cgtgctgtcg cggaccaatg cgttgtggcg
    19081 gattgaggcg ccggtctcgg atgccgcgga tcaggaagtg gctgacgaag ccgtgtcacg
    19141 gcttttgcgg cttgaggccg agggaggcga agaggcggcg tctcccgagg cgcaggctgg
    19201 cacggcggcc ccctccagat atgcgccggt cagtcatgtg gtgctcttcg gcgatcaggg
    19261 cgagacgcgt ttcaccgtca cgcgcgcgga gccggacgcc ccattcgtgc gcctcgcatt
    19321 caccaactcg gctgcgctgt atcgcgtggc cgccagcaat ctgccgtcgg ttttcatgag
    19381 tcaggacggg ctgatgtcgt tgcgggataa gaccgtgctg gccctgccgg aaacaacggt
    19441 gcggcgcgtc acgctcaaac gtgcggacgg gagcgttgaa accgcgcagc gcgactctgc
    19501 ggccgatgtc tggcgtctgg ctgatggaca gacgggcgtg ttgtccgcgg aacggctcgg
    19561 acggttgctc aagagcctga cctcgttgcg tgccgagcgt atcgaaaaga tcggcctgac
    19621 gccggaagaa ggccacgtgt atgggctgac gcgtccttgg ctggagatga gtgtcgatgt
    19681 ggacgcgaag gattcggtcc gcaagacgct cttggtgggg gatgaggcgg atctgggcag
    19741 ccggtatgcc gtggttcgcg ggcttgatgt gttgttcgtg ctgggaggcg atgcggtggc
    19801 ggagcttgcg gcgcattgga tcgagcccct gtgacgcgtg gggcaggggg tggaatgctc
    19861 gaaaccgtga tggagtttct gaacggaccg ctgggccggg aatgctttcg cctgaaccgc
    19921 gtgctgtttc tcgtgttcct tgcccttgcc acgctcgccg tgtggcgcgc gcctgcgccg
    19981 cgtcccggca aagtggggtt cggtctgttg cggggattga ctacggccgc attgctcgcg
    20041 attctgatct atcaggcgac ttggcagatc tcgggctttc tgaaaccgga gttcgttcat
    20101 ttcctgcggc ggtacaacaa gcggcccggg gcggccgcga tgcaggtgat gcgcggtccg
    20161 ttgctcgacc gccgcggcca tgttctggcg gcgccggttc ccggtgatgt gtggggccgt
    20221 cggtatccct tgggggaggc gggggttcat ccgctgggtt attaccattc tcaattcgga
    20281 atcacggcgg tcgagcgcgt gtgcgatccg gaattgagtg gctactccgt tgagcgccgg
    20341 gagggttttc ccggtgcgac gttgtttgcg gtgcgggccg aagaaggccg tgcggtgacg
    20401 ctcacgctgg acgcgcggct gcaagcgcgc gcctatgacc tgttggcggg acgcaaaggc
    20461 gcggtgatcg tgatgcgccc gcgttccggc gatttgctgg cgctggtcag ctcaccggga
    20521 ttcaatcccg gcgatcccgg tcccgcctcg cgggatgagg agaacgcacc ggtcttcaat
    20581 cgcgcgacac agggccgtta cccgcctggt tcggtcttca agatcctggt ggcggctgcg
    20641 gcgttggaca aagggttggc accggtctac gcttgtccgg ccatggggta tatcgccggc
    20701 cccaatacgc cgccgatccg cgacagcgaa tattacgcct acgcccgcag gggcgcggtc
    20761 tggccgggat ggggacggct caatctggag aaggcgctgg ttcactcttc gaatgtctac
    20821 tttgcccagt tgggcgtggc gtgcggtgcc gaagtcttta acgcgatgat ggcgcgtgcc
    20881 caaatcaacg cgccgcttgt ctatcttcaa gccgcctccg gcgaactccg gagcgccgcg
    20941 ggttcggcac cgacggtgac gcagccacgc aaactcgcga tgctcgcgat cgggcagggc
    21001 gatctgttgg tcaccccgct gcatgtggcc tgtttcactg cggccgtggc agcggacggt
    21061 gtgatgccgc ggccgcggtt gcgtaccgat gaggcgccgg gacggatggc cacgctctgc
    21121 tcgcctgaag cggcggggcg tcttacgggg atgctgcgtg cggcggtgcg taacggcacc
    21181 ggacgggctg cggagatccc gggactcgaa gtgtgcggca aaaccggcac cgcgcaggcc
    21241 gctggcggag atgatcacgc ttggttcact tgtttcgccc cgcgccgcaa gccgcgtcta
    21301 gttgtcacgg tgctggtcga gcgtggcggt ttcggtgccc ggagcgcact gccggccgca
    21361 cgcgcgttac ttgaggaagc ggttcgcctg ggccttgctc gacccgaagg ggagggggag
    21421 tcatgagcgc gcgccgtact cgtgtggtgt cgccggtcgg agcggtcgca ggcctgttcc
    21481 tgctttcgtt tctggcggta ctcagtgcca gcctctccgt gctcggtatc aaattgggca
    21541 acgcgcccgt gatgccgtgg gaacaactga tcccgctggg aatctacgcg gtcggcgtgc
    21601 ccgccgtact ggtgtggatg cgcgtcaacg ggtggaaagg cgacgcaggt ttggcggggg
    21661 cggtgtttct gctctgcggg ctcggggtga ccgttcaatt tcggatgggc agtttctctc
    21721 gcgggctggc agatccgatg gcgctcgcgc cgctcccgtt gggtctgctg gcctttgcgg
    21781 cgggcgtcgc gctgaccgcc aagcggcgcg gcgcgtggct ggcgcggacc ggttgggcgg
    21841 catatctcgc ggcgctcggt gtgttggccg ccttactgct gctgggccga aaatatcgcg
    21901 gcggactcta tctggcaggc aacatcaacc ctacggaact cgtcaaaccg cttctggttc
    21961 tgtttcttgc ctccttcctg agccgcagac agaaagcgtt ttcggaaacg caagtcggca
    22021 ttccggttcc ttctctggac gccttgctgg tcctgatctt cctctgggcg ggaccggtgc
    22081 tgctcactct ggcggtcaag gatctcgggc tgttgctcct gctcaacgcg attctggtcg
    22141 tgatgctgtt tgctgtggcg cgcagcaccg gatatcttgc cctcggactg gcggcggtaa
    22201 ctgccgcagg gtatggcgtg aggttccttt cgccgcacgt ccgcgcgcgt tttgacgtct
    22261 ggctaaatcc gtttgaagat ccgaccggca aggggtggca gattctgcaa gcgcttgcgg
    22321 cgatgtactc gggtggctgg tggggtgcgg gtttgggttc gggcgtgccg cagaccgtgc
    22381 cgatcgtgac ttccgatttt gtgtatgcgg cgctggcgga ggagatcgga ctggcggggt
    22441 gcgcgctgct gctggcggtt tatgccgtgc tcttttcccg cgggttccgt tgcgcgggcg
    22501 cggcgcgtac gccgtttgaa cggctgctgg gtgtcggcat gaccggtgcg ttggcgatgc
    22561 agacgatctt gaacgttgcg ggcgtgacca aggcgttgcc catgacggga atcacgctgc
    22621 cgctgatcag ccacggcggc agcagtttga tcaccacgct tctgatggcg gggctggtga
    22681 cgggcctgtc cgaccgccga tgatgccgcg tcgtgttcgc gttacggcat gggcgcgggc
    22741 gcggccgggg ctgtaggggc cgccggttgc tgggtgaagg gcaacgcctg ctcggctgcg
    22801 ggtgccgagg gtttcacccc ctccatgagg gatgagccgg tgtgcgcggt gaggcgcgag
    22861 agggccagtg tgttgatcag gaagatcgcg ccgagaataa tggtcgcttg agtcagcaca
    22921 ttccccatct gcgcgccgaa cagggcctca cccataccgc cgccgaaact gacgcccaac
    22981 ccgccctctt tcggcttctg cagcaggatg acggccgcca gcaggaaaca gacaaacact
    23041 tcgatcacat acaaaaagcc aatcagaatc tgcatgatgc gcactcccct tcgttttccg
    23101 gtagcctttg ccgtacggaa aatgtctttt tataacaaat agtgtgcatc tcccgcaatg
    23161 gcaaaaaacg cgtcccgagc ttttcgggtc aacaaaatgt gggcttgatc agatccgtcc
    23221 ggatgactaa cataacggta ccaactgggg tctgcgcatc tttgtttgga atcagatgat
    23281 gagattcaga atggttacag acttggcctc atggacgacc gagcgcttga tgttgagcct
    23341 tcggcgtcaa tttgcatatt cggtcggcgt gttctttctc atgctcccat tcttggtacg
    23401 gggcgagatt cgtggcatca tcacgttctc ggttctggaa atctgcgtcg ggttcacatt
    23461 cctttggcac tggcgggaac tacggcgaag acagagccga atggagacgg ataccaagta
    23521 gcaatcggtt gcaaaaccgc ttccatagcg gtacctttct tttcgtgggt cacggcttgt
    23581 cattcctttc tttgtttttg tggcacaaaa agaagaagga tagccgtggc ccgccttttt
    23641 caagccagcg gttcccgaag ggggggccac aggcccctat aggtgggggg aatttggaca
    23701 ggattaacag gatttacaag attggagatt aggtttcaag ccagcggttc ccgaaggggg
    23761 ggccacaggc ccctataggt ggggggattt ggacaggatt aacaggattt acaggattgg
    23821 agatcagggt atactggttg agggcgttgg ctgtataact attggtcagg gtgtttaccg
    23881 ccgaccagac gcggttgccg atggcatcgt agctgtaatc gtaattgtca gccctcagaa
    23941 ccgcgttcgt aacctcgcca ggcgcgttgt agaggaatgc gttgtgactg tggcataacc
    24001 cgcagtcttc ctgccaaacg cgtctgcccg taatgccatg gccgtaaccg aacaccgaaa
    24061 cagtcacgcc gtccaccatg ttgctgaccc ccgtgacaag atcggggcgg aaaccgtctc
    24121 gccaagcctt tcacccccga cagtcacacg cgtattctca accctgttgc gcccgtcaag
    24181 agt
//
LOCUS       DAOSLE010000022        23900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2356_length_23900_cov_8.397819, whole genome shotgun sequence.
ACCESSION   DAOSLE010000022 DAOSLE010000000
VERSION     DAOSLE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23900
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_23900_cov_8.397819"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PK576_02980"
     CDS             <1..1152
                     /locus_tag="PK576_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60585.1"
                     /translation="PGVEIRAIGDLYEDRVAKQVKVLTDKGLPAPKGYFGSQDAWRGM
                     CESNVIDLIYITTPWLWHAPMALYAMRCGKHAVTEVPAAMTVEQCWQLVDTAEETRRH
                     CMILENCCYGFNEMFALMLCRKGVLGELVHGEAAYIHDLRASHLREDHENGYQGQWRL
                     NWARQFSGNPYPTHGLGPVCQYMDINRGDKFEYLTSVSSGQFGISRAAAKKYGSNSPQ
                     AKAVYRQGDMNTSIIKTYRGRTIMVQHDTTSPRPYSRLNLISGTKGILSDYPLRVALE
                     PDPHRWLDDKKLEELKAKYTHPLWAKQGEYAKKMGGHGGMDMLMDMRLIHCLQNGLPL
                     DISVYDTALWSSVVGLSVDSVARDGRPVRVPDFTRGGWKTADPLEIVTL"
     gene            1226..1990
                     /locus_tag="PK576_02985"
     CDS             1226..1990
                     /locus_tag="PK576_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOE60586.1"
                     /translation="MKVEIAKSTEEMAVAAAACAAEGVRGAIARSGCARIIVATGASQ
                     FDFLKALVRAPEIDWTRVTGFHLDEYVGLPITHRASFRAYLRERFIAALPAPLAAFHE
                     VDGENPDPAAECRRLQNLLDAAPIDVACVGIGENGHLAFNDPPADFDAKAAYLVVDLD
                     EACRRQQVGEGWFASLEEVPKRAISMSIPQILKSRRIVCTVPDARKAPAVRNTIEGPV
                     SNLCPASILQTHADCTVFLDPSSAGELRSAKGVASV"
     gene            complement(2041..3186)
                     /locus_tag="PK576_02990"
     CDS             complement(2041..3186)
                     /locus_tag="PK576_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE60587.1"
                     /translation="MRHFHVLVLMEWYDHPNRVGIGRYAAEQNWYLTVSDGCVPPKGW
                     SGNGILTLINERNDIIDYVRSQRVPCVDFGAYRPDIPLARVCGDHRRIGLVAADHLTE
                     RGHTQAAYFSTGTGHPQMLRHAGFTERFEARTGHTPLPLVWAHRPGSANDDWQSLNRW
                     LKQELHRLPKPLAVFCYCDYDAAKVENACLEAGYKIPEDVAILGVDNDTLVCENLRIP
                     LSSVRHDRVRVGYEGSALLDRLMRGGTPPTEPVLIPPRGVELRASTDGFATADPIIRA
                     VIHFFREHLDRSIGVAEAAAQAGLPPRRLEEHFTRVLGQTVYTTLTRLRMFEVKRLLA
                     FSDLPVKEIARRTGFCHAQHLNNAFVRLERCTPLAYRKSESQAASRR"
     gene            complement(3330..6065)
                     /locus_tag="PK576_02995"
     CDS             complement(3330..6065)
                     /locus_tag="PK576_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60588.1"
                     /translation="MRRAIDVLMHEKNLDTQRIAVVGVGSVAKTVLWAAANDERIACV
                     AVLGASAHEKLLQLIAPRLLYVVPCTSKNRKEAEATFLSIRRAARLWETAYGLPGLGI
                     KTYPNPDFERGDGRIGFYESATPHSITRTGWVRYLDFVDRHLGKPATAADAPCEPASP
                     FKVTVSSEPKLPVVNYVKNGDYELGFKTYVFGPGAEKIMAITNDAYEGDCALWTAKGC
                     GHSINTRGVAPPGTTNRIAITVKLGPEHKGLTGLGAGFSQSYYAAPSTGGKLVPFAAS
                     GVNLTWQQAGSYTGWQRIESEPFVVPPDATNIVMNIHAGSLCYCATGGIVDNLFCGPA
                     WTDLSFVIENTTGLAQVTVEDENGDRVFCADAFPENLTRFERTIKVSAAMRYCVKSVD
                     RKAQSIAKWYPSPRPDSAAARAPLSVKGLRRLWPSNEKAAVFLVEMRDVCELAGASLV
                     AAVTATNGVEVMRKTFAPVLMQNRIEIPITELADGCYQLVASYHDAKGAAVTAKAPVD
                     FWKFPAMAGRPRDIYTIKDRNIYKNGRFFFPIFTWGGFPGWGTLRNHRFDTNAYIRCC
                     HDAIAEQKALGFNAILTDAWYFPDLARRAFSEIACRKYFPAWIPYTQVSQIPLDFLDI
                     ARMYDERGLAITPYLHIDRPDRVDDVDAWLKAVVNYRNADNILCWHLSDELDAAIAHN
                     RMLNDLHHEADPDRLTWINFINGARNNRDNGDILSEDPYPIPNERIAKVIWHAEKVRD
                     CTRPDQSVFQWLQMFGGEGTWTRSPTIAELEGMVYMSLNHGVRGLAYFGYTYSLADRD
                     GIRSMTEADYAKLGTLNRRVERLAPIYCLGQALERVYDNNLHYDRAVFRYAGRIWVSV
                     VNGLPEARHLRIPLPQDVKRVGKAEVVDDNRQISFKDGAIEEKFLPYAVRLYRLK"
     gene            complement(6457..8655)
                     /locus_tag="PK576_03000"
     CDS             complement(6457..8655)
                     /locus_tag="PK576_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60589.1"
                     /translation="MKRLFLFLFVLVLAVAGVVQAVETVAVPVLDSIPSLVDGMLTDD
                     CWSKALTLAPFTPIQPNQKPLVQKTEAKLFVTKDAFYLGLIGYTEDAAFTHRQSTDVK
                     AGPFGVDHFEIFVDPGHSGNLMQLAFGVNGCVYGNTSGRIRFGVQVYDDRYQIEVEIP
                     FDIFPLKRQGFNSEWLFNVARDAKLAGVDVCAGEYSSWSQLVSGTFHEADSFNRLTGI
                     GYNLAASFALQAAATKKPIELVAEKHLWMSDTVRIIAETNGKVPFVEISCAVSNSTGS
                     VVWQGKVKATGTKAAIEIPLKGCADGRYRFEAKFVDASGRTLVETHGVDFWKIPPKKT
                     VPRTIFTCRDNNIYKNGEFFFPIITWGGAPEWGVLRNHRFDTNAFFRCYHAILAEQKE
                     LGFNTILTGAEYYPDLARRALTDKTCRKYFPAWKDYRKIAQLPFDFNESIALHAEHNL
                     AIIPYLHIDKPQNAEDVESWLGSVLKWREADNILCWHLSDELDGAIDHNRMLNELHHD
                     VDPDRLTWINCIQGIAANRDNADILSTDPYPIPHSTVTMVGFHVKRLMDTCRPEQSPW
                     LWLQMFGGEGNWTRSPTFAELKSMVYITLNRGVRGIAYFIYKPDLVDRDGIRSITQED
                     YDKLGPMNRRVARLAKMYCLGEVIERQADDRKLTDVAVIKYDRSFWVSVVNGNTAKQR
                     VRVKLPVSLPQSGIADVDDENRTVSFQNSVIEEEFEPFAVHVYKIRHIAQ"
     gene            complement(8657..9274)
                     /locus_tag="PK576_03005"
     CDS             complement(8657..9274)
                     /locus_tag="PK576_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60590.1"
                     /translation="MMKKPMITLVALVVAIAAQAAELPLGTDTSRNYLKNGSFEIGRD
                     NFDYCGWQNNDPTGWSFSITNAASAGKRALFVEKAGSYLSQHAYVPGGTYVYTVTIRA
                     NPETDFQLGKGKPPKGLGMSYGANFRMEKGGKETRLGGHLLGGCPKDGTPAWQRLVSA
                     PIIIPTNIYGNCYMHIQCGVFHVPKGTGLIDDVALYRVNAPTDKK"
     gene            complement(9314..11329)
                     /locus_tag="PK576_03010"
     CDS             complement(9314..11329)
                     /locus_tag="PK576_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60591.1"
                     /translation="MKRLLIGLLAVSVFASSASSTVIYVSPYGSDETGDGSLENPYRT
                     PTYVIDNDSGRKVTTGCTVCILPGTYASVKSAYTDVKITVRGLHEPIGPNGETEPIID
                     GGYPLTEHRIFYLNNADIVVENLTFRNCCARYPGNQDGSAILGKFAAVRNCTFTNCVM
                     REAYHGGAISAQGTCEISNCTFVACKSIEPAYRNCSALFVSSGTATLVDCKFKYCTSV
                     GNSSGTACLSATGVVKNCTFEHNLARTQAGLYIGGGDYDISDCTFVDNVATEGSGAGI
                     TIDGTGAAGGRIVRCTFNGSKAAGNCALSVSGAPAWIENCTVSNAVAGFATCLAVTAP
                     ATVTNCVFTDCFSTNGGTTVQAKTSEQTFIDCIIADNRVEYRKNGEINTDYGAGTGVW
                     FSGGKKVFRRCSITNNYTTCWQAGGIYGEVTSEWYDCLVSSNSAGYYGGGTIRGNGTI
                     VSNCVFSENVARSGNCGGLYIQNGSDVLITDCAFIGNKAPTSIAGALTAPAGMTCRNT
                     LIVRNVSKNEPGGASIPKNSRWENCTVVGNVSSSGMCNLQSGDGAGSVFVNCLIAKDL
                     VSNAAGFWCGGSHTLTSCILASTDLSNALTEGSTYQVVYGAALKLGEDYRPICQSSPC
                     VDAGTDELAWIAGAKDLGGRHDRKIGEHVDIGCYEYKPWIGTTLYLK"
     gene            complement(11382..14432)
                     /locus_tag="PK576_03015"
     CDS             complement(11382..14432)
                     /locus_tag="PK576_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60592.1"
                     /translation="MHKSVFCGVFVAMLFASETWARTITCEGVKTDGVVTSFNLTFGA
                     SADGKTLGLYCAYGSEDCGSDPAAWPDLRRWATVPSTVNVLGAMLPPNWGVSVFALRF
                     FLFEEENASLGYDSRVDYLQSTGVQVIDSTYRANGNSRFVLEMTGMPSPLHSEGFVFG
                     YRSTHCAPDSFGLYQHCGKNHWKTPGWRYGTQIFTGSVDTNAHHIQNRCIVSNDLSRI
                     YVSQNGVATCINPDAPDDCGFTGSWNLAIFGAYYTSNRDTTGLVQTRIHSLSFYEGDT
                     AQREFIPVLKDGVPGLWDNVNGQFYPKTAGSADFLYGERLDNPVGATTFVSSSEPWVR
                     AEPNALFNIAASGMAIHGFGTLYTCGDGNNEVDVYLACAKSTEELPAPTLVWQSWKDD
                     LDTRFNTMIKGLEPSTAYNYSAYVMNPAGVKSLVTNGTFTTGPIIEVAVNEDPVSAVN
                     AAAAGSEVRIAEGTYVISGTMAPKASVAVVGQGAGAVLDANFVVDRRHVYTSVAHVTL
                     DNLTFKNARHETHTGYANWVHCGAAVRFGNNGGNSTSSQYAWITNCLFSANTNVNEGA
                     GGLFMECANKVVDCVFENNYGGPHPTSGLTGCGAAFVHHGNDYSQFSHCIFKDNDSGS
                     YGVVGSGTYTSGSALGMSRYGFVCNDCVFTNNTSTSGGILAGKISMVKRCEFIGNRSA
                     NGGVCWAGYPDSRWITNRFEQCVFRNNSASARGGVIASDYWRYFVFTDCAFIGNVAAT
                     AGSVFRGNGDFLCTGCLFTGNDSTSATLGNVADANGTMIAAQVPRLYDCTVSNNTATG
                     YSLVYVYNNADGGKFHNTRFIANLLSGADGTDIQRGVLWPHKASEVRNCLFAVNTNAC
                     GYGCAIALYSNSNEVDNCTVTGNRCYSEGNSGCVGIYRNTGTENRIRNCIVTDNRNLT
                     QDNLLHNFYGWGIDQVSYSLEDGTQLPNGVRGNINEADPRFVSPEKGDYSLFCRSPAR
                     NAGVNDDWMLGAKDLAGQDRILDDVVDMGCYEYRTIPGFMIRLH"
     gene            14921..15082
                     /locus_tag="PK576_03020"
     CDS             14921..15082
                     /locus_tag="PK576_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015113539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOE60593.1"
                     /translation="MQRYVCNVCGYVYDPEVGDPSNGIAPGTPFEALPEDWCCPDCGV
                     GKDQCEPAT"
     gene            complement(15161..16066)
                     /locus_tag="PK576_03025"
     CDS             complement(15161..16066)
                     /locus_tag="PK576_03025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOE60594.1"
                     /translation="MRYDQNHALLESSIPGLPPPKRGKVRDLYDLGDRLLLVATDRIS
                     AFDVVMPNGIPDKGRILNGLSVFWFSLLTETANHLITADVDAYPEPLQAVKEHLRGRS
                     MLVRKVSIIPFECIVRGYLTGSGWKEYQTHRTVNGQPLREGYRNGSKLDKVLFTPSTK
                     AEQGAHDMPVSAAELEAALGAEKAGRLAAASIRCYTKAAEYAAQNGILIADTKFEFGE
                     EGEGQLVLADEVLTPDSSRFWPQDQYRVGSNPPSLDKQFVRDWLESVRFNHQPPAPVL
                     PDDVVARTREKYVEAYERLTRRRFG"
     gene            16487..16912
                     /locus_tag="PK576_03030"
     CDS             16487..16912
                     /locus_tag="PK576_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE60595.1"
                     /translation="MNLNKVLSEAITKGATKVIRKPIAGIRRDVAQLKREIAELKRLI
                     RNAGRRQPSVAAARTTSAASEVPAMRPTAKMIRGLRKKLGVTQAQFAKLAGVSSLTVS
                     KWEAAPGRIRMRGHTLAGLAAVRAMGKREAKAALSKLES"
     gene            16983..17633
                     /locus_tag="PK576_03035"
     CDS             16983..17633
                     /locus_tag="PK576_03035"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOE60596.1"
                     /translation="MLRYTVKRVMPTSVASVAFGTAEKGRIGHVRPESSGHRPETDFR
                     VVHDGERLYVRFDVRDHYVRSVQTAYQAPVCTDSCVEFFVQPRPDKGYFNFEVNAGGT
                     LLLYYISDAARVEGGFRTFVKVPEEWGRQVEVRSSLPRVVEPERVGPLTWWVTIGIPF
                     ALFRAFVGGVSLSGAVWRANFYKCGDKTSHPHWLSWSPVPELNFHLPEAFGELALE"
     gene            complement(17635..18369)
                     /locus_tag="PK576_03040"
     CDS             complement(17635..18369)
                     /locus_tag="PK576_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60597.1"
                     /translation="MLRLLRLIAGVLLLPLCVAVTLALLDTLRSIPDTGVLLSAETRT
                     LLAGFFLWLGMWFFLPQPFRAYVVAHELTHAFWGALFGARVSDLRVSERGGSVRLSRS
                     NLLITLAPYFFPLYTILVVLLRLLAGLFLNPMPCPLLWLFFVGFTWCFHITFTLQSLM
                     TRQPDIQEYGRIFSYAVIYLFNLAGVGLWVVCTTTASFDTLATALALHTRTVCQQINQ
                     TVLLCSRQVYALLLPALKRLWIRPAA"
     gene            complement(18382..18678)
                     /locus_tag="PK576_03045"
     CDS             complement(18382..18678)
                     /locus_tag="PK576_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOE60598.1"
                     /translation="MNILLKIITRTTLFTAFLCVFAAGVIIIPPKMAQFKRLEQQRNE
                     LLRRIDHKKAEIKVLKEKQQRFKTDPEFVEAVARQNKRVRPGEMVFVFDSEDAK"
     gene            19006..21999
                     /locus_tag="PK576_03050"
     CDS             19006..21999
                     /locus_tag="PK576_03050"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60599.1"
                     /translation="MKKRFVYGVGAWVLAGAVLAGPRVRVEEELRRNLAVTAAGAVTE
                     AHVTALTFTPQETKRVRLVIRSTQGGEAGVDELEVFGPVGTNNLALAARGVRASASSV
                     IKGYAIHATAHLNDGLYGNDHSWIAGGTGEEWVEIELPAPVSVARVVFSRDRTGRYTD
                     RQPHEVEVRLSKDGRAWETVCKHTGSAQVLPTLMLPASALSEPTWDGVVTYAFLCERE
                     TWGTLDGKDELSPLRNDRPAVPGAEPYWGKLARLPHVARNLMQFDGLIMCLAEQGLDV
                     TAERKEAETLRARLQVEGETDALYLAVRHAKRRLFFRDPRLAPVERVLFAKRHPLQPS
                     HNYSEHMDGKFTSGGGICVLHVPRDAAGRLDPARAEVETLFDGNDGIVRHPVADFDAG
                     TVFFAYRPDKPEVKGWHSYWHLWSVNADGGGARKLTEGPFHDFDPVALPDGDLGFITT
                     RCAARFLCWQPQAYVLYRMNRHGGEMQRLSFANLSEWDPVMMRDGRILWTRSEYQDKG
                     ADFGHTLWSIRPDGTHPELVFGNNTPYGYGHAREVPGSAEIVCTLISHGDHQGPIALL
                     DRSKGLYEVAAITSITPDTRPQYQMGRSHHQTFRFPEPVSRDHFLVSHSPGRRSHWGL
                     YLIDRYGNRELLYLDPLISSKKPSLFRPRPRPPVMPPMRDAELAAQELGQFTVQDVYE
                     GLGPTVPRGRVKYIQVSQEMPAMLEQLSCGQYRNTHGPFMDFYATPVHKVKGPSHEIV
                     TRTRNALGAHAFRQGSAVTREDGTFLVTERGGWPSFVAKSVLGTVPVQADGSANFTAP
                     AGKVLYFHLLDEHFNEIQRMRSVLQLQPGERRSCVGCHEDRLQLPAMRQTTEALAQPV
                     RALDPSAWGGGPFDYAAVVQPVLDRRCVSCHDGMKKGRPDLRGTPDADFVPTSYRTLI
                     SGGWVHYFDWIWGARHFKAEPLTFGTLRSRLFDALKKEQHREVRLSAEERRTLTAWID
                     LNCPLWPDYRYRPERSGAPLQASVR"
     gene            complement(22054..23595)
                     /gene="hydG"
                     /locus_tag="PK576_03055"
     CDS             complement(22054..23595)
                     /gene="hydG"
                     /locus_tag="PK576_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOE60600.1"
                     /translation="MTAQTLTSAPLRRFANAADYRKRFIIREQIDRYLPGGRHYLDEA
                     ELERLLAAASAAPANDPVRVREILAKSRAIETLLPEETASLIQVTDPALLDEMETTAL
                     AIKHAVYDNRIVMFAPLYMSNLCVNRCAYCGFRCGNEDQKRRMLTMDEVRQEVDVLAG
                     KIGHKRLIAVYGEHPDTSTEYFAETIRTVYAHQVKSPQGTPVGIRRVNVNAAPLPIED
                     LRVLKQVGIGTFQVFQETYHRATYARLHPADTVKGDYDWRTTCFHRALEAGVDDVGMG
                     VLYGLYDWKFELLASLCHARDLERQFGIGPHTISFPRLEPASGTDLPTTSPYRIDDAT
                     LRRILIITRLSVPYTGMIVTCRERPESRDAFVRCGITQMDASSNIAIKGYSDFASETH
                     QEKHRQQFVLSDTRTLEELIAHLAREGIITSFCTAGYRCGRTGGCIMDALKTGKEGKF
                     CKINAVLTFREWLDDFASAATRMLCEPLLRRELAEIETTLPKFYPTVKEHYDRIANGE
                     RDLFF"
BASE COUNT         4906 a         6937 c         7334 g         4723 t
ORIGIN      
        1 ccgggagttg agatccgcgc gatcggcgat ctgtatgaag atcgggtcgc caagcaggtc
       61 aaggtgctga ccgacaaggg tctgcccgcg cccaaggggt atttcggttc gcaggacgcg
      121 tggcgcggga tgtgcgaatc caacgtgatc gacctgatct acatcactac gccttggctc
      181 tggcacgcgc cgatggcgct ctacgcgatg cgatgcggca aacatgcggt cactgaggtt
      241 ccggcggcga tgacggtgga gcagtgctgg caacttgtgg atacggcgga ggagacgcgc
      301 cgtcactgca tgatactgga aaactgctgt tacggcttca atgagatgtt tgcgctgatg
      361 ctgtgccgca aaggcgtgct gggcgagttg gtgcacggtg aggccgccta tatccacgat
      421 ctgcgcgcca gccatctgcg ggaagatcac gagaacggct atcagggcca gtggcgcctg
      481 aactgggcca ggcagttttc gggcaacccc tatcccacgc acgggctcgg ccccgtctgc
      541 cagtatatgg acatcaaccg cggcgacaag ttcgagtatc tcacctcggt cagcagcggt
      601 cagttcggca tctcgcgcgc ggccgccaag aagtacgggt cgaacagtcc gcaggccaag
      661 gcggtctatc ggcagggcga catgaacacc tccatcatca agacgtatcg cggccgcacc
      721 atcatggtgc agcacgacac gaccagcccg cggccctaca gccgcctcaa tctgatctcc
      781 gggaccaagg gcatcctttc cgactacccg ctgcgcgtgg cgttggagcc ggatccgcac
      841 cggtggctgg atgacaagaa actggaggaa ctcaaggcga aatacacgca tccgctatgg
      901 gcgaaacagg gcgagtacgc caagaaaatg ggcgggcacg gcgggatgga catgctcatg
      961 gacatgcgcc tgatccactg cctccagaac ggcctgccgc tcgacatcag cgtctacgac
     1021 acagcgctct ggagttccgt ggtggggctc agcgtggact cggtggcccg tgacggaaga
     1081 ccggtccggg taccggactt cacgcgcggc ggatggaaaa ccgccgaccc gctggagatc
     1141 gttacgctgt aaccccgaag cggacagggg aggggcacga tgcgtggcga aaaaggggaa
     1201 aagcagagag gaagagtaca tgggaatgaa ggttgaaatc gcaaagagta ccgaagagat
     1261 ggcggttgcg gcggcggcct gtgccgcaga gggggtccgc ggggcgatcg cgcggagcgg
     1321 ctgcgcccgc atcatcgtcg cgaccggcgc gagccagttc gattttctga aagcgctggt
     1381 tcgcgcgccg gaaatcgact ggacccgggt gacggggttt catctggacg agtatgtcgg
     1441 gctgcccatc acccaccgcg ccagtttccg agcgtatttg cgcgaacggt tcatcgccgc
     1501 gttgcccgcg ccgctcgcgg cgtttcatga ggtggacggc gagaatccgg atccggccgc
     1561 agagtgccgc cgcttgcaga acctgctcga tgccgcgcca atcgatgtgg cctgcgtggg
     1621 tatcggcgag aacgggcatc tggcgttcaa cgacccgcct gcggatttcg acgcgaaggc
     1681 ggcgtatttg gtggtggatc ttgatgaggc gtgccggcgc cagcaggtgg gcgagggctg
     1741 gttcgcctcg ctggaggagg tgccgaaacg ggcgatctcg atgtcgatcc cgcagatcct
     1801 gaagagccgc cggatcgtct gtaccgtgcc ggacgcgcgc aaggctccgg cggtgcggaa
     1861 cacgatcgag ggaccggtct cgaacctctg ccccgcctcg attctgcaga cgcatgcgga
     1921 ctgcacggtg tttctcgatc caagttccgc gggcgagttg cggtcggcaa aaggagttgc
     1981 gtcagtctga tgcggacgta atccgctccc ctttttccaa tgcgggggca gacgcttgtt
     2041 ttaccggcga gatgccgcct ggctctcgct tttgcggtaa gccagaggcg tgcagcgttc
     2101 gaggcgcaca aaggcgttgt tgagatgctg cgcgtggcag aagccggtgc ggcgcgcaat
     2161 ctctttcacg gggaggtcgg agaaggccag tagccgtttg acctcgaaca tgcgcaggcg
     2221 cgtcagggtc gtgtagacgg tctgacccag cacacgcgtg aagtgctcct ccagccgacg
     2281 gggcggcagg ccggcttgtg cggcggcctc cgcaacgccg atgctgcggt cgaggtgctc
     2341 gcggaagaaa tggatcacgg cgcggatgat cggatcggcg gttgcgaagc catcggtgct
     2401 ggcgcgcagt tccaccccgc ggggcgggat cagaaccggt tcggtcggcg gggtgccgcc
     2461 ccgcatcagc cggtccagca aggcagaccc ctcatacccg acgcggaccc ggtcgtggcg
     2521 gacgctggag agcgggatgc ggaggttctc gcaaaccagc gtgtcgttgt ccacgccgag
     2581 gatcgccaca tcctccggaa tcttgtaacc cgcctccagg caggcgttct ccaccttggc
     2641 ggcgtcgtaa tcgcagtagc agaagacggc cagcggtttc ggcaaccggt gcagttcttg
     2701 cttgagccag cggttgagag actgccaatc gtcgttcgcg ctaccgggcc ggtgggccca
     2761 gacgagcgga agaggcgtat ggccggtgcg ggcttcgaac cgttcggtaa accccgcgtg
     2821 gcgcagcatc tgcggatggc cggtccccgt ggagaagtaa gccgcttggg tatgtccgcg
     2881 ttcggtcaga tggtcggctg ccaccaggcc gatccgccgg tggtcgccgc agacccgggc
     2941 cagcgggata tccggccggt aggcaccgaa atccacgcac ggcacgcgct gcgagcggac
     3001 atagtcgatg atatcgttgc gctcgttgat gagggtcagg atgccgtttc cggaccaacc
     3061 cttgggcggg acacagccgt cgctaacggt cagataccag ttctgttccg cagcgtaccg
     3121 gccgatcccg acgcggttgg gatggtcgta ccactccatc agaacaagga catgaaaatg
     3181 gcgcataccc tcagtatagg ataaccgggc acgctgttca atgcgccggt atccggcacc
     3241 ggcaccagac gcgttgcctc acaattcatg acaccgaaaa tgaatcgcgc ctacgaccac
     3301 gctgactacg cagatgagag atacatatct catttcagac gataaagacg aaccgcatac
     3361 ggcaggaatt tttcttcgat tgccccgtct ttgaatgaga tctggcggtt atcgtcgacg
     3421 acttcggcct tgccgacacg cttcacgtcc tgcggtaacg gaatcctcag atgccgcgct
     3481 tcgggaagcc cgttcacgac ggaaacccat atcctgcctg catagcggaa gaccgcccta
     3541 tcgtaatgta aattgttatc atatactcgt tctagtgctt ggccaaggca atagataggc
     3601 gcaagtcgct ccacgcggcg gttcagtgtg ccgagctttg cgtaatcggc ctcggtcatc
     3661 gagcgtattc cgtctcggtc agccagggaa tacgtatagc cgaagtaggc aagaccccgc
     3721 acaccatggt tgagcgacat gtagaccatg ccctccagtt ccgcaatcgt cggcgaacgc
     3781 gtccacgtac cctcaccgcc gaacatttga agccactgga agacactctg gtcgggacgc
     3841 gtgcagtccc gaaccttctc ggcatgccag atcactttcg cgatgcgctc gttcggaatc
     3901 ggatacggat cttcggagag gatatcgccg ttatcgcgat tgttccgcgc accgttgatg
     3961 aagttgatcc acgtaaggcg gtccggatct gcctcgtgat ggaggtcgtt gagcattctg
     4021 ttgtgcgcaa tcgccgcgtc cagctcgtcc gacaaatgcc agcagagaat gttgtcggca
     4081 ttgcgatagt tgaccacggc cttcaaccac gcatccacgt catccacccg atcagggcga
     4141 tcgatgtgga gatacggggt gatggcgagg ccccgctcgt catacatccg tgcgatatct
     4201 aggaaatcca aagggatctg cgaaacctgc gtataaggta tccaggccgg aaaatatttg
     4261 cggcaggcaa tttcactaaa agcccggcga gcgagatccg gaaagtacca cgcatccgtt
     4321 agaatcgcat tgaaccccaa cgctttctgt tccgcaatcg catcatgaca acaccgaatg
     4381 taagcatttg tgtcgaagcg atggttgcgg agcgtccccc atcccgggaa gccgccccaa
     4441 gtgaagatcg ggaagaagaa cctgccgttc ttgtagatgt tgcgatcctt gatcgtatag
     4501 atgtcacgcg gacggccagc cattgccggg aacttccaga aatcgaccgg tgccttggcg
     4561 gtgacggccg cccccttcgc gtcatgatac gaggccacga gttgatagca gccatcggcc
     4621 agttccgtga tcggaatctc gatccggttc tgcatgagaa ccggggcgaa ggtcttacgc
     4681 atgacttcga cgccgtttgt cgctgtgacc gccgccacaa gtgaggctcc cgcgagttcg
     4741 cacacatcac gcatctcgac caagaagacg gccgcctttt cgtttgatgg ccagagacga
     4801 cgcagcccct tgacggacaa cggcgcacgc gctgcggctg agtccgggcg cggactcgga
     4861 taccacttcg cgatactctg cgctttgcgg tcgacggatt tcacgcaata gcgcatcgct
     4921 gcggaaacct tgatcgtcct ctcaaaacgg gtcaggttct ccggaaaggc gtccgcgcag
     4981 aagacgcggt caccgttctc gtcctcgacc gtaacctgcg caagtcccgt cgtgttctcg
     5041 atcacaaacg aaaggtctgt ccaggcgggt ccgcaaaaga ggttatcaac gattccgccc
     5101 gtcgcacagt agcagagcga gccggcatgg atattcatga cgatattcgt cgcgtcgggg
     5161 gggacgacga atggttccga ctcgatgcgc tgccaccctg tgtaagagcc cgcctgctgc
     5221 catgtaaggt tgacaccgct ggcagcaaac ggcacgagct tgccgcccgt cgatggggcc
     5281 gcgtaataag actggctgaa gcccgcgcca agaccggtga gtcctttgtg ctcggggccg
     5341 agcttgacgg tgatggcaat gcggttggtt gttccgggcg gtgccacgcc gcgcgtattg
     5401 atcgaatgtc cgcaaccttt cgctgtccag agggcacagt ctccctcgta agcatcgttc
     5461 gtgatcgcca tgattttctc cgcgcccgga ccgaagacgt aggtcttgaa accaagttcg
     5521 taatcgccgt tcttcacgta gttgacgacg gggagtttcg gctcggacga taccgtcacc
     5581 ttgaacggtg aggccggctc gcacggcgca tccgccgcag tcgctggctt accgagatga
     5641 cggtcgacga aatcgagata acgaacccag cccgtgcgcg ttatagaatg cggcgtcgcg
     5701 ctctcgtaaa acccgatgcg tccatcacct ctttcgaaat caggattcgg gtacgtttta
     5761 atgccaagcc cgggcagtcc gtacgccgtc tcccagagtc tggcagcgcg tcgtatggac
     5821 agaaaggttg cctcggcctc cttccggttc ttcgacgtac atggcacgac atacagcagc
     5881 cgaggcgcga tcaactggag cagcttttca tgagccgatg cgcctagaac agccacgcaa
     5941 gcgatacgct catcattggc ggcggcccaa agcaccgtct tggcaacaga accgacgccg
     6001 accaccgcaa tgcgctgtgt gtcgagattc ttttcatgca tcaagacgtc gatcgcacga
     6061 cgcacaccat ccgtatcagg cgcaacacga actacagcat atccacgacg caagatcaag
     6121 tccgccggaa acaccgcatt cgcatcatta ccgcagaaaa taataaccgg tgcaggacac
     6181 acagtggacg ttcgctgtgg acgcgatatc ttcgtcacat caatatcaga tgtctcaata
     6241 tcacccggac atggtgagga tatcacgtca cgcgttgacg ctgttccgaa aagattctgt
     6301 ttgcaccact gtatcgcctt gtcgcgtcca ccgtgctgcg gttgttccca gtagttgatg
     6361 cggatcggct cgtcatcagt cgcctgagtc tgcggtacga ggcaaaggac acacagggcg
     6421 attacaaagc acttcatgat atccagtctc aattgcctat tgtgcgatat gtctgatctt
     6481 gtatacatgg acggcaaacg gctcgaactc ctcttcgatc acgctgttct gaaacgaaac
     6541 cgtccggttt tcgtcatcga catcggcgat gccggactgc ggcagggaca cgggaagctt
     6601 cacgcgcacg cgctgctttg ccgtgttgcc gttcacgacg gacacccaga aggatctgtc
     6661 gtacttgatg acggcgacgt ccgtcagctt tctgtcgtcc gcctgccgct cgatgacctc
     6721 gccaaggcag tacatctttg caaggcgcgc gacgcggcgg ttcatcggtc cgagcttatc
     6781 gtagtcctcc tgcgtgatgc tgcggatgcc gtcgcggtcc accagatccg gcttgtagat
     6841 gaagtaggcg ataccacgca caccgcggtt gagtgtgatg tagaccatcg acttgagttc
     6901 ggcgaatgtc ggcgaacgcg tccagttgcc ctcaccgccg aacatttgca gccacaacca
     6961 gggggactgc tctggacgac aggtatccat caggcgctta acgtggaacc cgaccatcgt
     7021 caccgtgctg tggggaatcg gatacggatc ggtcgagagg atatccgcat tgtcgcgatt
     7081 ggcggcaatg ccctggatgc agtttatcca cgtcaggcgg tccggatcga catcgtggtg
     7141 caattcgttc aacattcggt tatggtcgat cgcgccgtcc aactcgtccg acaggtgcca
     7201 gcaaaggatg ttgtccgcct cgcgccattt cagcaccgaa ccaagccacg actcgacgtc
     7261 ttcggcattt tgcggcttgt cgatatgcag atagggtatg atcgcgaggt tgtgctcagc
     7321 gtggagcgca atcgactcgt tgaagtcgaa cggcaactgc gcgattttgc ggtagtcctt
     7381 ccacgccggg aagtactttc ggcaggtctt gtccgtgagc gcacgccgcg cgaggtcggg
     7441 atagtactct gcccccgtca ggatcgtatt gaaaccaagt tccttttgtt cggcgagaat
     7501 agcatgatag cagcggaaga acgcattcgt gtcgaagcgg tggttccgta acacccccca
     7561 ctcgggtgca ccgccccaag tgatgatcgg aaagaagaat tcgccgttct tatagatgtt
     7621 gttgtctctg caggtgaata tcgtcctcgg cacggtcttt tttggcggaa tcttccagaa
     7681 gtcaacaccg tgcgtctcga ccagcgtcct gccggaagcg tctacgaact tcgcctcgaa
     7741 acggtaacgt ccgtcggcgc atcccttcaa cgggatctcg atcgccgcct tcgtcccggt
     7801 cgccttgacc ttaccctgcc agacgaccga tccggtagag ttcgaaaccg cacacgaaat
     7861 ctctacgaac ggcaccttgc cattcgtttc ggcgatgatg cggaccgtat cggacatcca
     7921 cagatgcttt tcggcgacaa gctcgatcgg tttttttgtg gcagcggcct gtaaggcaaa
     7981 tgatgccgcg agattatagc cgatgccggt cagacggttg aagctatccg cttcgtggaa
     8041 cgtacccgat acgagctgcg accagctcga gtattcgccc gcacacacat caactcccgc
     8101 aagctttgca tcgcgtgcga cgttgaacag ccattcggaa ttgaagccct gccgcttcag
     8161 ggggaagata tcgaagggaa tctccacctc gatctgataa cggtcgtcat agacctgaac
     8221 gccgaagcgg atacgtcccg acgtgttacc atagacacag ccgttcacac cgaaggcgag
     8281 ctgcatgaga ttgcccgaat ggcctgggtc aacgaagatc tcgaaatggt cgacaccgaa
     8341 aggccccgct ttgacatccg tcgactgccg gtgcgtgaac gccgcatcct cggtatagcc
     8401 gatcagtccg agatagaacg cgtcctttgt cacgaacagc tttgcctctg ttttctggac
     8461 gagcggtttc tggttcggct ggatcggcgt aaagggcgca agcgtcagcg ccttcgacca
     8521 gcagtcgtcc gtcagcatgc cgtcgaccag tgacggaatc gaatcgagaa ccggcacagc
     8581 caccgtttcg accgcctgca caacgcccgc gacagccaat acgaggacga acaggaacag
     8641 gaaaaggcgc ttcatcttac tttttatccg ttggggcatt tacgcgatag agggctacgt
     8701 cgtcgatgag cccggtgcct ttcggtacat ggaagacgcc gcactggatg tgcatgtagc
     8761 aattaccgta gatattcgtc gggatgatga tcggcgcgga gacaagccgt tgccaggccg
     8821 gcgttccgtc cttcggacag ccgccaagca gatgtccgcc aaggcgcgtc tccttgccgc
     8881 ccttctccat ccggaagttg gctccatagc tcatgccgag ccccttcggc ggtttgccct
     8941 tcccgagttg gaagtccgtt tccggattcg cccgtatcgt cacggtgtaa acataggttc
     9001 cgcccggaac ataggcatgt tgactgagat aggatccggc cttctcaacg aatagcgcac
     9061 gtttcccggc cgacgcggca ttggtgatcg aaaacgacca gcccgtcgga tcgttattct
     9121 gccagccgca gtaatcgaag ttgtcccggc cgatctcgaa cgagccattc ttgagataat
     9181 tccgcgaggt atccgtcccc aatggcagtt ccgccgcctg cgccgcaata gctaccacca
     9241 aggcaaccaa tgtaatcatt ggtttcttca tcatcccatc tcccccttga ttttatctgt
     9301 cgacaaaaaa agatcacttc aagtacagcg tagtgcctat ccaaggctta tactcgtagc
     9361 aaccgatgtc gacatgctcg ccgattttgc ggtcatggcg tccaccgaga tcctttgctc
     9421 ccgcgatcca cgccagttca tccgtaccgg cgtccacaca aggagaggac tggcaaatcg
     9481 gacggtagtc ttcgcccagt ttcagggcgg caccgtaaac gacctgatac gtcgagccct
     9541 ccgttaaggc attcgacaaa tccgtgctgg ccagaatgca actcgtcagc gtatgcgacc
     9601 ctccgcacca gaagccggct gcgttgctga cgaggtcttt cgcaatcagg caattcacga
     9661 aaacactacc cgcgccatcg cccgattgaa ggttacacat cccgcttgaa ctcacgttcc
     9721 ccacgaccgt acagttctcc cagcggctat ttttggggat ggaggcaccg ccgggttcat
     9781 ttttgctgac gttgcgcaca atgagcgtgt tgcggcaggt catccccgca ggcgcggtaa
     9841 gcgctcctgc gattgatgtt ggcgctttat taccgataaa ggcgcaatcc gtaatgagca
     9901 catcgctgcc attttgtatg taaaggccgc cgcaattacc cgagcgggcg acgttttccg
     9961 agaaaacgca attcgacacg attgttccgt ttccgcgaat tgttccgccg ccataataac
    10021 cggcactgtt cgaagagacg aggcagtcat accattcgga cgtcacctcg ccatagatgc
    10081 cacccgcctg ccagcacgtc gtgtagttat tggtaatcga acaacggcgg aaaaccttct
    10141 taccgccact gaaccaaacg ccggtaccgg caccgtagtc cgtattaatc tcaccgtttt
    10201 ttcggtattc cacacgattg tcggcaataa tacagtcgat gaatgtctgt tcggatgtct
    10261 tcgcctgaac ggtcgtaccg ccgttagtcg agaagcaatc ggtgaagacg cagttcgtca
    10321 ccgttgccgg cgctgtcacc gccaaacacg tcgcaaaacc cgcgaccgca ttggaaaccg
    10381 tgcagttctc gatccaagca ggtgccccgc tgacggatag tgcgcagtta ccggccgcct
    10441 tgctgccgtt aaacgtgcaa cgcacgatac gccctcccgc ggctcctgtc ccatcgatcg
    10501 taatgccagc gccagagcct tcggttgcga cattgtcaac gaacgtacag tcggaaatat
    10561 cataatcgcc gccgccgata taaagacccg cctgcgtacg cgcaaggtta tgctcgaagg
    10621 tacagttttt cactacgccg gtcgccgaca gacaggctgt accgctggaa ttgccgacgg
    10681 acgtgcagta tttgaactta cagtcaacta aggttgcagt gcccgaactc acaaacaggg
    10741 cggaacaatt gcgatacgca ggctcgatcg acttgcaggc gacaaaggtg cagttcgata
    10801 tctcgcacgt cccctgcgcg gaaatcgcac cgccgtggta cgcctcgcgc atgacgcaat
    10861 tcgtaaaggt gcaattgcgt acagcggcga atttgcccag aatcgccgaa ccgtcctggt
    10921 tgcccgggta gcgggcgcaa cagttacgaa acgtcaaatt ctcaacaacg atgtcggcat
    10981 tgttcagata gaagatccga tgctcagtca aaggatagcc cccatcgata atcggctcgg
    11041 tttcgccatt cggaccgatc ggctcatgca gcccccgaac agtgatcttc acgtccgtat
    11101 aagcactctt caccgacgca taagtacccg gcaaaatgca aaccgtgcat ccggtcgtga
    11161 ctttgcgccc cgaatcattg tcgatgacat atgtcggagt ccgatacgga ttctcgagcg
    11221 aaccgtcgcc ggtctcatcg ctgccgtacg gcgagacgta aataactgtt gatgaggcgg
    11281 aagacgcaaa cacactgacc gccaataaac cgataagtag acgtttcaca ctaatttctc
    11341 ctgtttactc cccactcact cattgggcgc gcacggtccg tttaatgcag gcggatcatg
    11401 aagccgggga tggtccggta ctcatagcag cccatatcga cgacatcgtc aaggatgcga
    11461 tcctgtccag ccagatcctt tgcccccagc atccaatcgt cgttcacgcc ggcgttacgc
    11521 gcgggggacc ggcagaagag cgaatagtcg cccttctccg gtgagacgaa cctcggatcc
    11581 gcctcgttga tattgccgcg gaccccgttt ggcaattgcg tgccgtcctc gagcgaatac
    11641 gagacttgat cgatacccca cccatagaaa ttatgcagga ggttgtcctg agttaggttc
    11701 cggttgtcag tgacgatgca gttgcggatt cggttttcgg tgccggtgtt gcggtagatc
    11761 ccgacgcaac ccgagttacc ctcggaatag cagcgattgc cggtgaccgt acagttatcg
    11821 acttcgttgc tgttgctgta gagcgcgatt gcacagccgt agccgcaggc gttcgtgttc
    11881 accgcgaaaa gacagttccg gacctccgag gccttgtgcg gccagaggac accgcgctgg
    11941 atatccgtcc cgtcggcgcc gctcagcagg tttgcgatga accgcgtatt gtggaacttg
    12001 ccgccgtcgg cgttgttgta gacgtaaacg agactgtacc cggttgccgt gttgttggag
    12061 accgtgcagt cgtacagccg cggcacctgc gccgcgatca tcgttccgtt ggcatcggcc
    12121 acgttcccca acgtcgccga cgtggagtca ttgccggtga agaggcagcc ggtgcagagg
    12181 aaatcgccat tcccgcggaa aaccgagcca gcggtggccg cgacattgcc gatgaacgcg
    12241 cagtcggtga agacgaaata gcgccaatag tcagaggcga tcaccccgcc cctcgctgac
    12301 gcggaattgt tccggaagac gcattgctcg aaccggttgg tgatccagcg cgagtccgga
    12361 taccctgccc agcagacccc tccgttggcg gagcggttcc cgatgaattc gcagcgcttc
    12421 accatcgaaa tcttgccggc gagaatccct ccggaagtgg aggtgttgtt ggtgaagacg
    12481 cagtcgttgc agacgaaacc gtaccggctc atgccgagag cactgccaga cgtgtacgtc
    12541 ccgctcccca cgacaccgta ggagccggaa tcgttgtcct tgaagatgca atgggagaac
    12601 tgggagtaat cattgccgtg atggacgaac gccgcaccgc agccggtgag gccgctcgta
    12661 ggatgcggac cgccgtagtt gttctcgaag acgcagtcca cgaccttgtt cgcgcactcc
    12721 ataaagagtc cgcccgcgcc ctcgttcaca ttcgtattcg cggagaagag gcagttggtg
    12781 atccaggcgt actgcgacga agtggaattg ccgccgttgt tgccaaaacg gaccgccgcg
    12841 ccgcagtgga cccagttggc gtaaccggtg tgcgtttcgt ggcgcgcgtt cttgaaggtt
    12901 agattgtcga gcgtcacatg cgccaccgag gtataaacgt gacgccggtc gacgacgaag
    12961 ttcgcgtcca gcaccgcgcc cgcgccctgc cccacgaccg cgacgctcgc cttcggcgcc
    13021 attgttccgg aaatgacgta ggtcccttct gcgatgcgta cttccgaacc cgccgctgcc
    13081 gcattgactg cggacaccgg gtcttcgttt accgcgacct cgatgatcgg gccggtcgtg
    13141 aaggtgccgt tcgtcaccag actcttcacc cctgcgggat tcatcacata ggcggagtaa
    13201 ttgtaggcgg tcgagggttc caagcctttg atcatcgtat tgaagcgcgt atcgaggtcg
    13261 tccttccagc tttgccagac gagagtcggc gcgggaagct cttccgtcga tttcgcacag
    13321 gcaagataga catcgacttc gttgttgccg tcgccgcagg tataaagcgt gccaaagccg
    13381 tggatcgcca ttccgctcgc ggcgatgttg aacaatgcat tcggctccgc acgaacccag
    13441 ggctcactcg aagagacgaa cgtcgtcgcg ccgaccggat tgtccagacg ctctccgtaa
    13501 aggaaatcgg cgctacccgc agttttcgga tagaactgac cgttcacatt gtcccagagc
    13561 cccggcacgc cgtccttgag gactggaatg aattcgcgct gcgcggtgtc gccctcgtag
    13621 aacgaaagcg aatggatgcg cgtctggacg agtccggtgg tatcccggtt gctggtataa
    13681 tacgcgccga agattgcaag gttccacgag ccggtgaagc cgcagtcgtc cggcgcatcg
    13741 ggattgatgc aggtggcgac gccattctgc gacacataga tccggctcag atcgttgctg
    13801 acgatacaac ggttctggat atgatgagcg ttcgtgtcga cgctgccggt gaagatctgg
    13861 gtgccgtaac gccagcccgg cgtcttccag tggttttttc cgcaatgctg gtaaagaccg
    13921 aacgaatccg gcgcgcaatg agtcgaacgg taaccgaaga caaaaccttc cgaatgtaac
    13981 ggagagggca ttccggtcat ctcaagaacg aaacgcgagt tcccgttcgc ccgataggtt
    14041 gagtcgatga cctgcacacc agtggactgg agataatcga cgcgcgaatc atagccgagc
    14101 gaggcgttct cctcttcgaa gaggaagaag cgaagcgcga aaacactcac gccccagttc
    14161 ggaggaagca ttgcgccaag cacattgacg gtcgaaggca cggtcgccca gcgtctgaga
    14221 tccggccatg ccgcaggatc gctgccgcaa tcctcggaac cgtacgcgca gtagaggccg
    14281 agggtctttc cgtctgccga cgcaccgaat gtcagattga acgaggtgac gactccgtcg
    14341 gtcttcactc cttcgcatgt aatcgtgcgg gcccacgtct ccgatgcgaa cagcatggcg
    14401 acgaacacac cacagaacac acttttatgc atacaaccct ccttttgttg aaaatcagta
    14461 cgtgaggcgg acgagaccat ggggaggccg gaacgcgggc attacagtaa tggtcacgtt
    14521 taccataatg ataaggtact gcatttcccg tcgtgggtca acctgcggat aacgcaatct
    14581 caaaggctgt gagactgtga ggcggtgagg gtgtgaggct gttaggctgt taggctgtga
    14641 ggcggtgagg ctgtgagggt gtgaggctgt gaggcggtga ggctgtggtt aaatggtcga
    14701 tcccgatagc gatagcgatc ccgaccccga tttggatgga accctgaaac cttgaaacca
    14761 ggaaccctga aacccagaaa ccagaaacca gaaaccagaa accctgaaac caaagagcgt
    14821 tgctttcaac gctcttttta ggtcatggca ggaatgcgcg aatccggttg accggaatga
    14881 gaaaagaagg ttaacttacg acccgaaagg agcgtgcgat atgcagcgat acgtgtgcaa
    14941 cgtgtgcggt tacgtgtatg accctgaggt gggtgaccca tcgaacggga ttgcgccggg
    15001 aactccgttt gaggctttgc cggaggactg gtgctgtcct gattgcgggg taggcaaaga
    15061 ccagtgtgaa ccggcgacgt gaggcgcggg cagactgagg ccggcaacaa cggtgaggcg
    15121 ccgggtttcc gtcgcctcac cccgttttat cgggacgggt tcatccgaag cgtcgccggg
    15181 tcagcctctc gtacgcttcc acatatttct cgcgggtgcg cgccaccaca tcgtccggca
    15241 ggaccggcgc gggcggctgg tgattgaagc gcacgctctc cagccagtcg cggacgaact
    15301 gcttgtccag actgggcggg ttgctgccga cccggtattg gtcctgcggc cagaaccggc
    15361 tcgaatccgg cgtcaacacc tcgtcggcta acacgagctg gccttcaccc tcttcgccga
    15421 attcgaattt ggtgtccgcg atgaggatac cgttctgcgc cgcgtactct gcggctttcg
    15481 tgtagcacct gatcgaggcg gcggccaggc gtccggcctt ttccgcgccc agcgcggctt
    15541 ccagctctgc ggccgagacc ggcatatcgt gcgcaccctg ctcggctttg gtgctggggg
    15601 tgaagagaac cttgtcgagc ttcgagccgt tccggtaccc ctcgcgcaac ggttggccgt
    15661 tgacggtgcg gtgcgtctga tactctttcc agcccgagcc ggtgagatac ccgcgcacga
    15721 tacattcgaa cgggatgatc gaaacctttc gaaccagcat ggagcggccg cgcaaatgct
    15781 ctttcaccgc ttgcagcggt tccggatacg cgtccacatc cgcagtgatc aggtggttgg
    15841 cggtctcagt caggaggctg aaccaaaaga cgctcagacc gttcaggata cgccccttgt
    15901 ccgggatgcc gttgggcatc acgacatcaa aggcgctgat ccggtctgtg gcgaccagca
    15961 acaggcggtc gcccagatca tacaggtcac gcactttacc gcgtttcggc ggcggcagac
    16021 ccggaatcga actttcgagc aaagcatggt tttgatcgta tcgcatagac acctgctggt
    16081 taaatgacgc acagtttagc gtaaaaaccg gttaaatcca gtactggatt tgaaaaagaa
    16141 tccgggttcg ccccgttcgc gttttcgtgg gcggatatgg atcaagcgtt caatcttaag
    16201 ataaggcaag ccgggatttc cgacagcttc gcttcggcgg gaagaaaacc gggattaaca
    16261 gaatttatca ttgaggtcat agacgaggat gggttgtcga aacacgcaac ctcaacgtgc
    16321 aatgactcgg atgcgccccg ttaccctcgg cgtatgaaaa gcgaaaaagc gaaaatcacg
    16381 gacttgacgc agaggggggg gtaaaggtat gatataagtt tgttggcctt gctggccgtt
    16441 aggcatacgt tgccgataat agatcacctc aaaaacggag taccccgtga atctgaataa
    16501 agtcctatct gaagccatca cgaaaggcgc aaccaaagtg atccgtaagc cgatcgctgg
    16561 aatccgtcgg gacgtggccc aactcaagcg cgaaatcgcg gaactgaagc ggttgatccg
    16621 gaacgccgga cggcggcagc cttctgttgc cgccgcacgg accacctccg ccgcaagcga
    16681 agtgccggcc atgcgcccca cagccaagat gatccgcggt ctccggaaaa aactcggcgt
    16741 tacgcaggcg cagttcgcga aactggccgg ggtctccagc ctcacggttt cgaaatggga
    16801 ggcggcgccc ggacgcatcc gcatgcgcgg tcacaccctt gccgggctgg ccgccgttcg
    16861 ggccatgggc aagcgcgagg cgaaagcggc gctttctaaa ctcgaatcct gatattccga
    16921 aaccgacatg cgcgcgcgct ggcgggatgt gccggcgcga cggacgcgcg ggagaggcgg
    16981 gtatgctgcg gtacacggtg aaacgggtga tgccgacatc agtggcgtca gtggctttcg
    17041 ggacagcgga gaagggccgg atcgggcatg tgcgtcccga atcctcgggc caccgtcccg
    17101 agacagactt ccgggtggtg catgacggcg aacgtctgta tgtccgcttt gacgtgcggg
    17161 atcattacgt gcggtcggtc cagaccgcgt accaggcgcc ggtctgcacg gacagttgcg
    17221 tggagttttt tgtgcagccc cgtccggaca aggggtattt caattttgag gtgaacgcag
    17281 ggggcacgct gctgctgtat tacatctcag atgccgcgcg ggtggaaggc ggcttccgga
    17341 cgtttgtgaa agtgccggag gagtgggggc ggcaggtgga ggtccgctcc tccctgccgc
    17401 gcgtggtcga gcccgagcgg gtgggtccgc tgacgtggtg ggtgacgatc ggaatcccgt
    17461 ttgcgctgtt tcgggccttt gtgggtgggg tctcgctttc cggcgcggtc tggcgtgcca
    17521 acttctacaa atgcggggac aagacctcac acccgcactg gctctcttgg tctccggtcc
    17581 cggaactcaa tttccatctg cccgaggcgt tcggtgaact ggcgctggag tgactcaggc
    17641 cgcaggtcgg atccacagcc gtttcagggc cggcaacagc aacgcataca cctgccggga
    17701 gcagagcaga accgtctgat tgatctgctg gcagacggtc cgggtgtgta gcgccagcgc
    17761 cgtcgcgaga gtatcgaaac ttgcggtggt ggtgcagacc acccagaggc ccacccccgc
    17821 gaggttgaag aggtaaatca cggcgtagga gaagatccgc ccatactctt ggatgtccgg
    17881 ttgccgggtc atcaggcttt ggagcgtgaa ggtgatgtga aaacaccagg tgaaccccac
    17941 gaaaaagagc cagagcagag ggcagggcat ggggttcagg aaaagtccgg cgagcaggcg
    18001 cagcaggacc accagtatcg tgtagagggg aaagaaatag ggcgcgagcg tgatcagcag
    18061 gttgctccgg ctcagacgca cgctgccgcc ccgttccgag acccggagat cgctgacccg
    18121 tgcgccgaac agcgcgcccc agaacgcgtg ggtgagttcg tgcgccacca cgtaggcgcg
    18181 gaaaggctgg ggcaggaaga accacatgcc gagccagagg aaaaatccgg ccagcagggt
    18241 gcgcgtctcc gccgacaaca gcacaccggt gtccgggatg ctgcgcaggg tatcgagtaa
    18301 agcgagcgtc acggccacgc atagcggcag caacagcaca ccggcaatca gtctgagcag
    18361 acgcaacatg ccgtcgcgat cctacttggc atcttctgaa tcaaacacaa acaccatttc
    18421 gccgggacgc acccgcttgt tctgccgcgc gacagcttcg acaaactccg ggtcggtctt
    18481 gaagcgttgc tgcttctctt tcagcacctt gatctcggct ttcttgtggt cgatccggcg
    18541 caacagctcg ttgcgttgct gctccagccg tttgaactgc gccatcttcg gcgggatgat
    18601 gatcaccccc gccgcaaaca cgcataaaaa tgcggtgaag agcgttgtcc gggttatgat
    18661 tttgagcaga atgttcacgc ggtcagcccg taggtcgcaa tccggttgtt tgattgtgag
    18721 tactcaatcg catattcgct taaaagtcaa tcgcgaattt tccgtaatcg ccaaattgtg
    18781 cgattcgacg gttgacgcaa aaaaggcggt gcggtaccgt atttccaccg ttgggaatcg
    18841 cttttgcagg ataaagtcgt gtaatagggc gggaagagag cgcatgcgga cttaacacga
    18901 cgttaccggt aggggggacg agaggtttgg aaacccatga cgaagaaaaa cgtcaaaaga
    18961 tcggttgcgg cgcgaacggg cagcgatcac cgcgcgggga taggcatgaa aaaacggttt
    19021 gtgtacgggg tgggcgcatg ggtgttggcg ggagcggttc tggccgggcc gcgcgtacgc
    19081 gtggaagagg aactgcggcg gaatcttgcc gtcacggcgg ccggagcggt gacggaggcg
    19141 catgtgaccg cactgacgtt cacgccacag gagacgaaac gtgtccggct ggtgatccgc
    19201 agcactcaag ggggcgaggc gggggtggat gaactggaag tgttcggtcc tgtggggaca
    19261 aataatcttg cactcgcggc gcggggcgtg cgggcgagcg cctcgtccgt catcaaaggg
    19321 tatgcgatcc acgcgaccgc gcatctgaat gacggactgt acggcaatga ccacagttgg
    19381 atcgcgggcg gtaccggcga agagtgggtt gagattgagc tgcccgcacc ggtatccgtg
    19441 gcgcgcgtcg tattctcgcg cgaccgcacg ggccgataca ccgaccgcca gccgcacgag
    19501 gtggaagtga ggctttcgaa agatggtcgg gcgtgggaaa cggtctgcaa acataccggt
    19561 tccgcgcagg tactgccgac gttgatgttg cccgcatccg ccttgagcga accgacatgg
    19621 gacggggtcg tgacttacgc ctttttgtgc gagcgcgaaa cgtggggtac gctggatggc
    19681 aaggatgagt tgtcgccatt gcgcaacgac cgtccggccg tgcccggcgc ggaaccgtat
    19741 tggggcaaac tggcgcgcct gccgcatgtc gcacggaatc tgatgcagtt tgacggtctc
    19801 atcatgtgtc tcgctgaaca aggtctggat gtcacggccg aacgcaaaga ggcagagacg
    19861 ctccgtgccc gcctgcaggt tgaaggcgaa acggatgcgc tctatctggc cgtccgccac
    19921 gccaagcgcc gcctcttttt ccgcgatccg cggctggcgc cggttgagcg cgtgctcttt
    19981 gccaagcgcc atccgttgca gccgtcgcac aactacagcg agcatatgga tgggaagttc
    20041 acgtcgggcg gcggtatctg cgtgctccat gtgccgcgcg atgcggcggg acgcctcgat
    20101 cccgcgcggg ccgaggtgga gacgctcttt gacgggaacg atggcatcgt gaggcatccg
    20161 gtggcggatt tcgatgcggg gaccgtcttc ttcgcgtacc ggcctgacaa gcccgaggtc
    20221 aaagggtggc attcgtactg gcacctctgg tccgtcaacg ccgacggcgg cggtgcgcgc
    20281 aagctcacgg agggaccctt tcatgacttc gatccggttg cgttgccgga cggcgatctg
    20341 gggttcatca ccacccgatg cgccgcgcgc tttctttgct ggcagccgca agcctacgtg
    20401 ctctaccgta tgaaccggca tggcggggaa atgcaacggc tctcgttcgc gaatctaagc
    20461 gagtgggatc ccgtgatgat gcgtgacggg cggatcctct ggacccggtc cgaatatcag
    20521 gacaaaggcg ccgatttcgg gcacacgctc tggtcgatcc gtccggacgg cacccatccg
    20581 gagttggttt tcggcaacaa cacgccatat gggtacggtc acgcgcgcga ggtgccgggc
    20641 agcgcggaga tcgtctgcac actgatttca cacggcgacc atcagggccc gatcgcgttg
    20701 ctggatcgct ccaaggggct gtatgaagtt gccgcgatca caagcatcac gccggacacg
    20761 cggccgcaat accagatggg ccgttcgcat catcagactt tccgtttccc ggagccggtc
    20821 tcacgcgacc attttctagt cagccacagt ccgggtcggc gctcgcactg ggggctctac
    20881 ctcatcgacc gctacggcaa ccgcgagttg ctctacctcg acccgttgat cagcagcaaa
    20941 aagccctccc tgttccgtcc gcgtccccgg ccaccggtga tgccccccat gcgggacgcg
    21001 gagctggccg cgcaggagct cgggcagttc acggtgcagg atgtgtacga ggggctcgga
    21061 ccgacggttc cgcgaggccg ggttaaatat atccaggtct cgcaggagat gcccgcgatg
    21121 ctggagcaat tgagctgcgg gcagtaccgc aacacgcacg gtccgttcat ggatttctac
    21181 gcgacgccgg tgcacaaggt caagggtccg tcacacgaga tcgtgacgcg cacacgcaac
    21241 gcactggggg cgcacgcttt ccgtcagggc agtgcggtaa cacgggagga cggcacattc
    21301 cttgtgactg agcgcggcgg ctggccgagt tttgtggcca agagcgtgct gggcacggtt
    21361 ccggtgcagg cggacggctc cgccaacttc accgcgccgg ccggcaaggt tctttacttc
    21421 catctgttgg acgagcactt caacgagatc cagcgcatgc gcagcgtgct gcaactccag
    21481 ccgggcgagc ggcggagttg tgtcggttgc cacgaggacc ggttgcagtt gccggccatg
    21541 cggcagacga ccgaggcact ggcgcaaccg gtacgggcac tggatccgtc ggcgtggggt
    21601 gggggaccgt tcgattatgc ggctgtggtg cagccggtgt tggatcgacg ctgcgtatcc
    21661 tgccacgacg gtatgaaaaa aggacgcccc gatctgcgcg ggacaccgga cgcggatttt
    21721 gtcccgactt cttatcgcac gctgatctcg ggcggatggg ttcattattt cgactggatt
    21781 tggggtgcgc ggcacttcaa ggccgagcca ttgaccttcg gcaccctacg cagccgtctg
    21841 ttcgacgcgc tgaaaaaaga acagcaccga gaggtcaggc tcagcgcgga ggaacggcgc
    21901 acgctgacgg cgtggatcga cctcaactgc ccgctgtggc ctgactaccg ctaccgtccc
    21961 gaacgttccg gcgcaccgct ccaggcttcc gtccggtagg gcacgaggtt ttgagtgaga
    22021 ggatgaaaag ggaaagcatg gagatacggt ccgtcagaag aagagatcgc gctcgccatt
    22081 ggcgatccgg tcataatgct ctttgacggt cgggtagaat ttcggcaggg tggtctcgat
    22141 ctcggccagt tcccgccgca gcaacggctc gcacaacatg cgtgtggcgg cactggcgaa
    22201 atcgtcgagc cactcacgga aggtgagcac cgcgttgatc ttgcagaact ttccttcctt
    22261 gccggtcttg agcgcgtcca tgatgcagcc gccggtgcgt ccgcaacgat acccggcggt
    22321 gcagaaagag gtgatgatgc cttcgcgagc caagtgcgcg atcagttctt cgagggtccg
    22381 tgtgtccgag agcacaaatt gctggcggtg cttttcctgg tgggtctcgc tggcgaagtc
    22441 gctgtaacct ttgatcgcga tgttgctgct cgcgtccatc tgggtgatcc cgcagcgcac
    22501 gaacgcatcg cgcgattcgg gccgttcgcg gcaggtcacg atcatgccgg tgtacggcac
    22561 ggagagacgc gtgatgatga ggatacggcg gagggtggcg tcatcgattc gatagggcga
    22621 ggtggtcggc agatccgttc cgctggcggg ttccagccgc gggaaggaga tcgtatgcgg
    22681 gccgataccg aactggcgtt caaggtcgcg cgcatggcag agtgaggcga gcagttcgaa
    22741 cttccagtca tagagcccgt acagcacacc catgcccaca tcatccaccc ccgcctccag
    22801 agcgcggtgg aaacaggtgg tgcgccagtc gtaatcaccc ttcaccgtgt cggcggggtg
    22861 caggcgcgcg tatgtcgcgc ggtggtacgt ctcctgaaag acctggaaag tgccgatgcc
    22921 gacttgcttg agcacacgca ggtcctcgat gggcagcggt gccgcattga cattcacgcg
    22981 ccggatgccg accggcgtgc cttggggcga cttgacttgg tgtgcgtaaa cggtgcggat
    23041 ggtttcggcg aaatactcgg tcgaggtgtc cgggtgttcg ccgtagacgg cgatcaggcg
    23101 tttgtggccg atcttgcctg ccagcacgtc gacttcctgc cgaacctcgt ccatggtcag
    23161 catgcggcgc ttctgatcct cgttaccgca acggaatccg cagtaggcgc aacggttgac
    23221 gcagaggttg ctcatataga gcggggcgaa catcacgatg cggttgtcgt agaccgcgtg
    23281 tttgatcgcc aaagccgtgg tctccatctc gtcaagcagg gcgggatcgg tgacctgaat
    23341 cagggatgcg gtctcttcgg gtagcagggt ttcgatggcg cgtgatttcg cgaggatttc
    23401 acggacgcgg accgggtcgt tcgcgggcgc ggctgaggcg gcggcaagaa gccgctccag
    23461 ttccgcctcg tcaaggtagt gccggccgcc cggcagatag cggtcgatct gttcccggat
    23521 gatgaaacgt ttgcggtagt cggccgcatt ggcgaaacga cgcaacggag cggatgtcaa
    23581 ggtctgtgcg gtcatgaggc ctcctcggtt tgatttctgc ggggcagggg aggcaccgga
    23641 ccgggttcga aaagcggccg cgccgacagg ccgtcgaccg gatatgcggt tcctcgggtc
    23701 agagccaggc tcctccacct cagaaaacgc atctacggta cacgttcgag cggtcaaacg
    23761 caagattcaa accccaaata aattgaacgt cgcaagtcaa aaaaagaggt gtctcaaatc
    23821 cgtgttggta atgtgggtgg tgtggtgaaa caatcgatct cgatagcgat accgatagcg
    23881 ataccgattt ggatggaacc
//
LOCUS       DAOSLE010000023        23658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2385_length_23658_cov_8.718553, whole genome shotgun sequence.
ACCESSION   DAOSLE010000023 DAOSLE010000000
VERSION     DAOSLE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23658
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_23658_cov_8.718553"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(525..2387)
                     /locus_tag="PK576_03060"
     CDS             complement(525..2387)
                     /locus_tag="PK576_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE60601.1"
                     /translation="MRKRTMFWSFFGAHVVLLLLAVGAVSFYNWHISRYAFMRQWVRE
                     LEMQAKTAAALLPDDGGEVDEQYAAQFFERLRHHGIHRFTLILPNGRVLGDSDVDVEN
                     MESHEDRPEVIEAFEKGLGMRQRYSASLGKQMLYVAYRLPQEGAARAVIRVAVPVRFL
                     TSEMRAADKLLLSFFLGVLCVTLALSYVAALRITRPVSSLQLGLARIGSGNLSYRLPV
                     PRVPHLANLARSINRNAERLQRQVQALHEERNLRAMILENMTRGVIAIDRDRCVLDIN
                     AAARALLDIGDEQKVIAKPLGERIRNPEVFALIEACEQQNQMCEGEMTIDGKVTRRLQ
                     LRVSMLNDLGGERIGSLLVFSDVTLLRRLETVRQDFVSNVSHELRTPVTSIKGFAETL
                     LDGAVKDPEKAERFLKILVRQAGQLETTIRDLLELSRLEGHSPKALDCQRVPVGELLK
                     NAAELCQERADKRSVTLSLSCDEGLTVMVHAGLMEQALVNLIDNAITYGTDGTQKAQV
                     DIEAVSDGSAVSISVRDYGPGIESKHLDRLFERLYRVDKGRSRELGGTGLGLAIVKHI
                     ALIHNGSVDVESKVGKGSVFTIRLPLQCPDHETQEIKIDREPGAQVSSPAGIVR"
     gene            complement(2384..3079)
                     /locus_tag="PK576_03065"
     CDS             complement(2384..3079)
                     /locus_tag="PK576_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE60602.1"
                     /translation="MANESILIVEDEADIRELLRYHLEREGFQVSECGSAEDAKKFLA
                     RRLPDLVILDLMLPGTDGYAFCRMLRADERTAEIPVIMVTARDEEADIVAGLEVGADD
                     YLTKPFSARVLMARVRAVLRRAAAEPEDGEGVLSHGAIEIDRTYHTVKVDGKPVALTL
                     SEFKILNLFMKRPGAVFSRYQIVDAVHGQDYPVTDRSVDVQIVGLRRKLGKYGGLIET
                     VRGVGYRMKLERP"
     gene            complement(3051..3758)
                     /gene="phoU"
                     /locus_tag="PK576_03070"
     CDS             complement(3051..3758)
                     /gene="phoU"
                     /locus_tag="PK576_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOE60603.1"
                     /translation="MNPIFMREQEGLKEKILLLAGDVEMRLTEVLKATEQRDKDALYR
                     WIDRDSEIDGKEVQIEEECLKILALHQPVAKDLRFVVAVLKINNDLERIGDIVVNIAQ
                     KGIPLADTPVGDIFSSVIQMGRVSREMVKDALDALVNLDLEKANAVIRRDDDLDTMRS
                     NVWETVAQRATATNVGSITESLLLLNGVARDLERIGDHATNIAEDVAYLVSGKIVRHL
                     RRDVKEDKHGERKHSDS"
     gene            complement(3779..4531)
                     /gene="pstB"
                     /locus_tag="PK576_03075"
                     /pseudo
     CDS             complement(3779..4531)
                     /gene="pstB"
                     /locus_tag="PK576_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
     gene            complement(4614..5891)
                     /gene="pstA"
                     /locus_tag="PK576_03080"
     CDS             complement(4614..5891)
                     /gene="pstA"
                     /locus_tag="PK576_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOE60604.1"
                     /translation="MASFLFIVLVPILWRGLGAYVFQGTVEHRRMLMGEFNRGSEESL
                     AAELKEVRQARAPVYQALEAFEKELAEMPASRRKELRPVFQSVRDGVASLLGSMPGEP
                     LPALPRRIYGKTRWDQARHGARLILFEEVWEHDDDGSHRRLEPRKALFAGTALEPLFD
                     LLEEKNLRRLMRPRLTLYARFLTDVSVDAHFFGGIGPEVMGTFYLALGAMLIAVPLGL
                     IAAIYFQEYAKGGPTMAFLRSCVNTLAGVPSIVFGLFGLAFFINTIKVSPGKSVLAGA
                     LTLSLLVLPTVIRAAEEAIAAVPQSYKEGALALGAGKWHTIMTVILPAALPGILTGIV
                     ISMGRAAGETAPIIFTAAVSVGKMVKASELFVMPTPALSWNLYNLCTEHEAVDEIRHV
                     QAGMAATLVLIVLALNLAAILLRARVAKRLKGG"
     gene            complement(5967..6887)
                     /gene="pstC"
                     /locus_tag="PK576_03085"
     CDS             complement(5967..6887)
                     /gene="pstC"
                     /locus_tag="PK576_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOE60605.1"
                     /translation="MRRKTIGFLGRSFLFAVTSVAAVAVFAIILFIARDALPFFRDRT
                     SLEFFVSKSWYPSHTPPQFGAAAIFFGSFLVTAAAVILAVPSGVMAAICLSDILPFKA
                     RQLVKPVIEILAAIPSVVYGFFALTVVAPFLQNAGEWLPGNIEIASGVNALNASVILA
                     IMALPTIVSVSEDALQAAGRELREGSYALGATRSETLLKVVIPAAFSGIFSAVLLGIM
                     RAMGETMVVWMASGNAAQIPKPWYNLLQPVRTLTATIAGDMGEADQMSGSAHYHVLFV
                     MGLCLLLFSFGCNIAGEWVVARQRKKLRGE"
     gene            complement(6936..7748)
                     /locus_tag="PK576_03090"
     CDS             complement(6936..7748)
                     /locus_tag="PK576_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE60606.1"
                     /translation="MKGSLLVAGLVACCAVAQAQDKLTLDGSTTVGPIAKAFAEFYMR
                     QNPGVNITVSESGSGNGAKALLNGTCDIANLSRNLKDAERKAMEEKGVKPVEHTVAFD
                     ALPVIVHPSNRVKGLTLEQVRDIYTGKITNWKEVGGADQRIVVISRDTNSGTYETFKD
                     LVLGKDNKILESAEYTGSNGGVRQRVQMTRGAIGYAGLGFVDRTVKALEINGVAPCAE
                     SVVQKRYPISRALFMFTNGEPAADTLAAKFIGLSKTARGKEIIEEIGFVPVP"
     gene            complement(7843..9231)
                     /locus_tag="PK576_03095"
     CDS             complement(7843..9231)
                     /locus_tag="PK576_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOE60607.1"
                     /translation="MRKRWLCAGLTVLAMPVLAQDGAAATEKKAELDFDAGMDIRARY
                     EFKDNWMNKGKTSVNPAYEDYYRLRTRFWGKATFGDDYAAFLRLANEFRDYRNSTGNK
                     RKNEFPDELFIDNLYLDFNNLADRVDLRVGRQDIREGAGRVISDGTPGDGSRSTYFDA
                     LFAKVRFLEKSDVDLMATWNRYRDDWTLGNPHDVYDNTKIKSGSPYSKMDEKGLMAYV
                     HYNEIENFPMEFYWIWKQEDRFFDKKTRYPGRDFHTLGTRLMPKINEKLSAEVEAAVQ
                     TGQIDSQSGMRGRDILAWMTYAGLTYSEKEVFSKPKLTGAVLWMSGDTDSYYKTQDGS
                     TDTGWNPVFNRTTWFSELCSSMYDQYRWSNLIYPHLEAAVEPFQKHKVKVQCGPMFAH
                     EKDNGATSSYRGLYTQARYDFPLLSKIFGKRGEVKGAVVGEALWYGDYYQHDAVNEDV
                     ATWLRFELNGKF"
     gene            10063..10224
                     /locus_tag="PK576_03100"
     CDS             10063..10224
                     /locus_tag="PK576_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60608.1"
                     /translation="MIETSRDSAAAFQELIGNSRFSSSHPGTLSIENSGKSLVYTAPK
                     KVGSVIYIQ"
     gene            10352..12568
                     /locus_tag="PK576_03105"
     CDS             10352..12568
                     /locus_tag="PK576_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,3-alpha-glucanase family
                     glycosylhydrolase"
                     /protein_id="HOE60609.1"
                     /translation="MKKWIIAVALAAVNGFAGTNPERKVYAHFMGCWPVFGCGGQDKS
                     MKAFLTTGEAVECGPVGGRFNNFPLVPQEGFNMKLLDKAKLEIKRAIRGGIDGFAFDA
                     WAGDQSPQTLDVYFQAAEELKVDFGLTICFDPSCHGAKWIEGTNMCEKYATSAKWVLR
                     HLDSPNLARFHGKPLFFGYYSKGIVKDSGSFLTAEKRREIKAAWDAWRKALPCEVYLH
                     GSIEGYVDWKNFERNDYPAIAKDCAETFDAVGAFTGWDANWANRNDLWKHIRDAGCGW
                     SQPIVPQYCNKRGGIITGAGLDVLHQCWTNAIDRGSELLQFVTWNDYGEETILGPAYG
                     SNYTFLRVNRHYADWLKQGKPPVIEKDEVHVVFRRCCDPQKTTFPFLDRRINLPTALE
                     VITFLTKPGKVVVEGYGKYEAPAGMFSKKFDEHPGNVSVKVLRRNWLFLNQTVCQLTA
                     PEEISAKKWREDDVAATWSSTYDEEWALDFPGIEPLYYSENGDLDGDGLPNWFEMVYF
                     GEFPYMKTASGADPNADPDGDGFTNLQEYENETNPLIPDTPYASGFVWSLQELAERAL
                     IGNPARDSKGRYVWRREYQYGAKGHDYVPGSAFKEILSGGGSLQSQMTHVYAPNPFGG
                     GGFDCGIQFNKPEKGQAEARVHRDSPIALTWIAPVACTVDVVTKVTPKNPKVKARTTL
                     VLGDKVLGTTVARKIQVGKGDEIRLVIENLNPWSGGDLFAIEKFDVIWAKESERAN"
     gene            complement(12679..12753)
                     /locus_tag="PK576_03110"
     tRNA            complement(12679..12753)
                     /locus_tag="PK576_03110"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12719..12721),aa:Asn,seq:gtt)
     gene            13000..13869
                     /gene="dapA"
                     /locus_tag="PK576_03115"
     CDS             13000..13869
                     /gene="dapA"
                     /locus_tag="PK576_03115"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOE60610.1"
                     /translation="MTLTGTFTALVTPFNTDGSVDYPALHALVQWQIENGVTGLVPVG
                     TTGESPTLEIDEHIRVITATAEAAAGRAVIIAGTGANATAEAIELTRQVLNAGVQATL
                     QVTPYYNKPNAEGLYRHFAAVADLGLPVVLYNVPGRAGREIPLDVVIRLAAHPNVAAI
                     KEAAGSVDRVSAIRNACELPVLSGDDSLALPMISVGACGVISVASNVIPKEMSKLVQL
                     ALANDLVEARALHQTYYPLFRDLFIDTNPIPVKTALTLMNRVGPTFRLPLCEPSEKIR
                     AQLVQTLTHLNLM"
     gene            13926..14735
                     /gene="dapB"
                     /locus_tag="PK576_03120"
     CDS             13926..14735
                     /gene="dapB"
                     /locus_tag="PK576_03120"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOE60611.1"
                     /translation="MKIAILGAAGRMGQNLLRCAEKIGACQIVAATECPGHPLLGQSV
                     RQAFGLTAENIPETLRIEETWPKDTEVVIDFTFHTTVTNNLARAIACRQAYVLGTTGF
                     TEAERQTILAATQQIPMVWAPNMSLGVNLLFELVRRAASVLGPEYDAEIVEIHHRRKK
                     DAPSGTALGLAEALARGRNVKLDEVACYGRQGIPGERPEGEIAIHALRGGGVVGDHTV
                     LFIADEERIELTHRANSRESFAAGALRAALWLEGRPAGLYTMRHVLGFESV"
     gene            14753..15265
                     /gene="folK"
                     /locus_tag="PK576_03125"
     CDS             14753..15265
                     /gene="folK"
                     /locus_tag="PK576_03125"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOE60612.1"
                     /translation="MATPREAILSLGSNLGDRRIALERACAALEEEPGLRILERSPIY
                     ETEPVGVPEEEAGRLFLNQVVIVSTDRSPEALSDTVHAIEARWGRTRTGVHGAARVLD
                     IDLIAFGTERRNTPELTLPHPRTRERRFVLQPLADLRPNLVLPGESRSVAELLRDLPD
                     TPRVRRIQQA"
     gene            complement(15904..17175)
                     /locus_tag="PK576_03130"
     CDS             complement(15904..17175)
                     /locus_tag="PK576_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60613.1"
                     /translation="MMGSRRSFLKTAAASAAAWNIIPARVLGKDAPSNRIAMAMIGVG
                     GMGQGNMSSFLGMKDVIVRMVCDVNTSKTEQAKQKVDKHYGNSDCLVTKDFREVCARG
                     DIDAVMIATPDHWHAYVGTFAARHGKDIYGEKPFTHDLREGRALVRAVEQHGRIWQTG
                     SWQRSSSEMRRAVELVRNGRIGKIARVEVGLPPGGRPRNPIDPHATIPKGLDWDFWLG
                     PAPYRPYQGIADWDWRWVLDWGGGQMMDWIGHHADIAQWGLDRDTSGPVTFEGYAPFE
                     VDGICDSPKTYRFSCRYADGVEMTVADSRQVKSGTTWYGENGEWVWVDRGRFDASTPE
                     IRDSRIEPGELRLHSPGHHRNFIDCVKTRATTLTPAEVAHRSASIGHLGQIAMLTGRK
                     IKWDPVREQILGDVSAEALLGRTPRGPWSLV"
     gene            complement(17216..20377)
                     /locus_tag="PK576_03135"
     CDS             complement(17216..20377)
                     /locus_tag="PK576_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOE60614.1"
                     /translation="MYNRNVILRTLCIIYAISGAALVASAQTLHPVPQADAERIGELV
                     ETWVPARSSRARRVLVFWRCEGFVHGRAIEYGNRALEIAASQTRAFTADFSREYEALT
                     PERLARYDALVLNNTTALNTRQHGFLESALTAFVRSGKGLAVIHAGADNFYHAEQAAE
                     MVGGRFWGHPWGDGGMWAFKLDEPDHPLNRAFGGQGFLFGDEIYQQQSPFYNRAKLRV
                     LVSLDLSHAATAAATGQRRADNDYAVSWIRPYGRGRVFYTSFGHDARAFLDRATLIHI
                     LDGVQYAVGDFRVDDTPSGLRAADLDRVRAATDLTVAEIAHDLREILTCTGHAAVDAA
                     NRALLIGLLKENGVPPYGRQAVLRAMTAAGETVPVDVLATCLAIPETREGALAQLAAT
                     PGEASSRVLSLAVPVAGPELRCALLNALAQRREAAAIAPYVADTEERVATAALAGLGR
                     IGDKTALAVLQKPVRPALEPVRLTALAACLGTLAEQGGTRAAARAARPLFAHAGTPFP
                     VRAAAARVLLQADCGFFAEGMADGCPHVRQTVVRAADRVPLRKLVAALHTAAPTDQVA
                     LLAKLAERDARSYVGEVVAKLEGEQEIVVVAALHALGRLGGCKEVPVMVSLITREGVV
                     GKAAREALLALRAPGVGAALLDVAARDPALQTRLLTLVGERGEVALLPRIRPCLRSER
                     AEVRKEAWKTLGLLADGQMYATLFTELRHLQAEEQVQAEAALQAAGKRVDPAARSVVL
                     LDTWGEKTLGVPARNALIALMAVYRDPAFVAPLSTALKEMDAGVREGALRALADWTDM
                     RPYPVLKEAVSAQPDARLRQLALRGAVKLVSAHGGAETQGRFLELLRTAPDDKGRESV
                     AEAMFLRDGLGFFPVLQTLFGDAACGPFAKRLYLRFYDEKVKSRADQPWREVAPSLWR
                     ANASHAAADAAKAFDRKPETRWSSNRASEEGMWFTLDLGENLCVSQVVLDTTRSASDT
                     PNGFEVLISDDGKTWQGPVAKGDGTQKEKNVIPMLTQGRHLKIVTTGGRPGKHWSIHE
                     IQIMAGFDPKKVAEIRQVAESLR"
     gene            complement(20865..21674)
                     /locus_tag="PK576_03140"
     CDS             complement(20865..21674)
                     /locus_tag="PK576_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60615.1"
                     /translation="MTLCTHRICGFIRLLLLSLAFPWGGLCGGREEVLAKGWTLDLLI
                     ERFNQREKVVIAYDLEHPSSKNWGGDGPYFSRLFGMIEKLGDRAALPFLEEKSFMTNA
                     PVFVRSRAAEAYVKIADVEEGFEFMRKIYETQFVQGSRRYFLNKKFLEKVGAEEKTLT
                     PETKENIASFLLGIVQNVESSNEANDADRFLLDRISEYANSKQRATLVRYANTGNEWV
                     TNTYNPIKAHFDKIPPGQRIDLRERFPNLPPLPDDAPAGDGGSIKALSPSL"
     gene            complement(21784..22824)
                     /locus_tag="PK576_03145"
     CDS             complement(21784..22824)
                     /locus_tag="PK576_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60616.1"
                     /translation="MRGFAMFRSAAVFLALAAFGADVTAAGHVFNPSGLPLGGTGRFK
                     IGVSANVPDADITWTVTEGAGCVAFVGDNTGPEVAVHATATGDFRLEANIKNLFITPP
                     HVQPFFTGTVRPAVNVPVTVWIVRDDDGKNPARQAAEIPGLLADANRILWPSGLTLVQ
                     SGAVHYLNNAAWLNHTDVLNPANTNLAAMLNATNSMGNAVELYFVEMLEGEDAAGVCW
                     PEGIAIARNGNGHTIAHEVMHSCGLEDIYTVENPYGPDPNPVPGPVSEERIPADWCGG
                     YYPPGLTQRSLITRLLMRGGAFGPEPPVSEGICLPRGTVYGWRHAGGGNVRTLGNAGV
                     GLSAIQRNPGSH"
BASE COUNT         4875 a         7487 c         6621 g         4675 t
ORIGIN      
        1 gcgattccga tcccgattcc gcactaacgc gggcggagcc cgcaatctca aagactgtga
       61 ggagttgaga ctgtgaggct gtgaggctgt gagactgtga ggagttgaga ctgtgaggct
      121 gtgaggtttt acaaacaagg caataacgaa tgattaacca cgaaagagaa cttcgttccc
      181 catccgttcg ctccgccgcc ttctccctca cagccttaac ccctcacagc cttaacccca
      241 acgcgggcgg agcccgcaat caaaagactg tgaggctgtt tgatgcgacc cgccgcatat
      301 tccctcacag ccttaacccc tcacagcctt aaccccaacg cgggcggagc ccgcaaccaa
      361 aaggctgtac aaaccttgtt aagccgtaac gcactaacgt accaacgcac taatgcacta
      421 atgaactaat gcactaatta actaatgcac taattaacta acgaacttct cccgctcttc
      481 gtgtgagaaa caacctttta ttggccttat cggccggcaa ggcgctaacg aactattcct
      541 gcaggcgagg acacctgcgc tcccggctct ctgtctatct tgatttcttg tgtttcatgg
      601 tccggacact gaagaggcag ccggatggtg aaaaccgagc ccttgccgac cttgctctca
      661 acatccacgc tcccgttgtg gataagggcg atatgcttga cgatcgccag ccccagtccc
      721 gtgccgccca attcacggct gcgccccttg tccacccggt agagccgctc gaacagccgg
      781 tccaggtgtt tgctttcaat cccgggaccg taatcgcgca cggaaatgct cacggccgag
      841 ccatcgctca cggcctcaat atcgacttgg gctttctgtg tcccgtccgt accgtatgtg
      901 atggcattgt cgatcaggtt cacaagcgcc tgttccatca acccggcatg aaccataacg
      961 gtcagcccct catcacacga gagcgaaagc gtgacgctcc gcttgtcagc acgctcctga
     1021 cagagttccg ccgcattctt caagagttcc ccgaccggca cccgctgaca gtcgagcgct
     1081 ttaggcgaat gcccctccaa tcgggacagc tccagcaagt cacggatggt cgtctccagt
     1141 tgccccgcct gcctcacaag gattttgagg aagcgttccg ccttttcagg gtctttaacc
     1201 gccccatcca gaagggtctc ggcaaagccc ttgattgatg tcaccggcgt gcgcaactca
     1261 tgcgaaacgt tcgacacaaa atcctgccgc acggtttcca atcgccgcaa caacgtgaca
     1321 tcgctgaaca ccagcaagga gccgatccgt tccccgccta agtcgttcaa catcgacacc
     1381 cgcaactgca gccgtctggt caccttgccg tcgatcgtca tctcgccttc gcacatctgg
     1441 ttttgctgtt cacacgcttc gatcaacgcg aaaacttcag gattccgaat ccgttcgccg
     1501 agcggtttgg caatcacctt ttgctcgtcc ccgatatcca acagcgcacg ggccgccgcg
     1561 tttatgtcaa gaacgcagcg gtcacgatca atcgcgatga caccccgtgt catgttctcc
     1621 agaatcatcg cccgcagatt acgctcctca tgcagtgcct gaacctgccg ttgcaggcgc
     1681 tccgcattcc ggttgatgga ccgcgccaaa ttcgccagat ggggaaccct cgggaccggc
     1741 aaccggtacg acaagttccc actgccgatt cgcgccaatc ccaactgaag cgatgatacc
     1801 ggacgcgtga tccgcagggc agccacataa ctcagcgcca atgtcacaca taagacgccc
     1861 agaaaaaatg acaacagcag tttgtcagca gcgcgcatct ccgaagtaag gaagcgcacc
     1921 ggcactgcca cacggatcac cgcgcgcgcg gccccctctt gcggaagccg atacgccaca
     1981 tagagcatct gcttccccag gctcgcgctg taccgctgcc gcatacccag acctttctca
     2041 aatgcctcga tcacttcagg gcggtcttca tgcgactcca tgttttcgac atcaacatct
     2101 gagtccccca aaacacgtcc gttcggcaga atcagcgtga aacggtgtat gccgtgatga
     2161 cgcaaccgct cgaaaaactg ggccgcgtac tgttcgtcga cctctccgcc gtcatccggc
     2221 aacagggccg ccgccgtctt ggcctgcatc tccagttcgc gaacccactg ccgcataaaa
     2281 gcgtacctgc tgatatgcca gttatagaag gagaccgcac cgaccgcaag cagcagcagc
     2341 acgacatgcg cgccgaaaaa gctccagaac atcgttcttt ttctcacggc cgctccaatt
     2401 tcatccggta ccccaccccg cgcaccgttt caatcaatcc gccatatttc cctaacttac
     2461 ggcgcaaccc cacaatctgg acatccaccg accggtcggt cacaggataa tcctgaccgt
     2521 gcaccgcgtc cacaatctgg tatcgcgaga acaccgcgcc gggccgtttc atgaagagat
     2581 tcaatatttt gaactcgctc aatgtcagcg ccaccggctt gccgtcaacc ttaaccgtat
     2641 gatacgtgcg gtcaatctcg atggcgccat ggctcagcac cccctccccg tcctcaggct
     2701 ctgccgctgc acggcgtaaa accgcccgca cgcgggccat cagaacccgc gcgctgaacg
     2761 gcttggtcag ataatcatcc gcccccacct caagcccggc cacgatatcg gcttcctcgt
     2821 cccgcgccgt gaccatgatc accggaatct cggcggtgcg ctcatccgcg cgcaacatcc
     2881 ggcagaaagc gtatccgtcc gtgcccggca acatcagatc gaggatcacc agatccggca
     2941 acctgcgggc caaaaacttc ttggcatcct ctgccgaccc gcattcggag acctgaaagc
     3001 cttcacgctc aaggtgatag cgcaacagct cacggatatc cgcctcgtct tcaactatca
     3061 gaatgctttc gttcgccatg cttatcctct ttcacgtctc ttcgcaaatg acgcacgatt
     3121 ttcccgctca caagatacgc gacatcctcc gcgatgtttg tggcatgatc gccaatccgt
     3181 tcgagatcgc gcgcgacccc gttcagcagc agcagcgatt cggttatgct gccgacgttt
     3241 gttgccgtgg cccgttgcgc cacggtttcc cagacgttgc tccgcatcgt gtccagatca
     3301 tcatctcgcc ggatcactgc gttcgccttc tccaaatcca agttcacgag tgcgtccaaa
     3361 gcgtccttca ccatctcacg ggaaacccgc cccatctgga tcaccgaaga aaagatgtcg
     3421 cccaccgggg tatccgcaag cggaattccc ttttgcgcga tattgaccac aatatccccg
     3481 atgcgttcca agtcgttgtt gatcttcaaa accgcgacca caaaacgcag atccttggcg
     3541 accggctggt gcagggccag aattttcaaa cactcctcct cgatctggac ctccttcccg
     3601 tcgatctccg aatcccggtc tatccagcga tataacgcgt ccttgtcacg ctgctccgtc
     3661 gctttcagca cctcggtcag acgcatctcc acatcgcctg ccaaaagcaa aatcttctcc
     3721 ttcaagcctt cctgctcccg catgaaaata ggattcattt gcgaccctcc cgttctcctc
     3781 aaccgaacct tccggtgatg tagtcttccg tatgcttctc tcggggcctc gtgaacagct
     3841 ccttggttgg agcgaattct acaagatcgc cctcataaaa aaaagcggtt ttatccgaga
     3901 cccgggcagc ctgttgcatg ttatgcgtga cgatcgcgat cgtgtagtta ccccgcaact
     3961 ggacgatcag ttcctcaatc cgcgccgtgg ccttcggatc caaggcggac gtcggctcat
     4021 ccatcagcaa catctcgggc ttgacggcca acgcccgcgc cagacagagc cgctgcgcct
     4081 ggccacccga caaccccaac gcattcttgt acaagcggtc tttgacctcg tcccacaacg
     4141 ccgcaccaac cagactctcc tcgacaatca catccagctc cgctttggtg tgcctcccgt
     4201 gtaaccgtgg accgtacgcg acattatcgt aaatgctttt cgggaacggg ttgggcttct
     4261 gaaagaccat accgatcctg cgccggagaa tcgccgtgtc cacatcactc ccgtacacat
     4321 tctgcccgtc aaaatataga tcgcccgcag tatggcaacc cggtatcaga tcgttcatgc
     4381 gattgatcgc ccgcaaaaac gtgctcttgc cgcacccgct aggaccgata atcgctgtca
     4441 ccagcccgca cggcacagcc aagtccacct tccggaccgc ctcgtgcgaa ccgtagaaaa
     4501 cggaaaaacc gcgagcttcc acatgcgtct tcacctttgt cgcattttca ttcattttac
     4561 cactctcaca ctctcatcct tcaagccttc agcacttccg cacgttatcg tgctcacccg
     4621 cccttcagcc ttttcgccac ccgcgcccgc aacaggatcg cggccagatt caacgcgagc
     4681 acaatcagca ccagcgtggc cgccatgccg gcctgcacat gccggatttc atccacggcc
     4741 tcgtgctcgg tacagagatt gtacaggttc caagagagcg cgggtgtggg cataacgaaa
     4801 agctcagagg ccttcaccat cttgccgaca ctcacagcgg cggtgaaaat gatcggcgcg
     4861 gtctcccctg ccgcccgtcc catgctgatc acgatcccgg ttaaaatgcc cggcagcgcc
     4921 gctggcagaa tgaccgtcat gatcgtgtgc catttgcctg cgcccaaggc caaggcgccc
     4981 tctttgtacg actgcggcac agccgcgatc gcttcttcag cggcacggat caccgtcggc
     5041 agcaccagca aggagagggt cagcgcaccc gccaaaacac tcttgccggg tgagactttg
     5101 atggtgttga taaaaaacgc gagtccgaac aggccgaaca cgatactggg cacgccggcc
     5161 agcgtgttga cgcaggaacg cagaaacgcc atcgtcggac cccctttggc gtactcctga
     5221 aaatagatag ccgcaatcaa gcccaacggc acggcgatca acatggcacc caaggccaga
     5281 taaaaggtgc ccatgacctc cggcccgatc ccgccgaaga aatgcgcgtc caccgagaca
     5341 tccgtcaaaa aacgcgcgta caaggtcaac cgcggccgca tcagccgcct caggttcttc
     5401 tcctcaagca gatcaaacag cggttccaaa gccgttcccg cgaacaacgc tttacggggt
     5461 tctaagcgcc gatggctgcc gtcatcatca tgctcccata cttcctcaaa caaaatcaga
     5521 cgcgcgccat gccgggcctg atcccacctt gtcttgccgt aaatcctgcg cggcaaagcg
     5581 ggcaacggct cgcccggcat cgaacccaag aggctggcga ccccgtcccg cacactctga
     5641 aacaccgggc gcaactcttt acgccgcgat gcaggcatct cggcaagttc tttctcaaat
     5701 gcctccagag cctggtaaac cggtgcgcgc gcctgccgca cctccttgag ttctgcggca
     5761 aggctttcct ccgatccccg gttgaattcg cccatcagca tgcggcggtg ctccaccgtg
     5821 ccttgaaaca cataggcccc gaggccgcgc cataagatcg gcaccagcac gataaacaga
     5881 aacgacgcca tcaccgccat tgcgagaagc gcgcatgctg tgaaggcccg gtctgtaaag
     5941 gttctaaagg aacggcgcat cctgtcttac tctccccgaa gttttttccg ctgacgggcg
     6001 accacccatt caccggcaat gttgcagccg aaactgaaaa gcaacaggca caaccccatg
     6061 acgaacagca catgatagtg ggcggaaccg gacatctgat ccgcctcccc catgtctccg
     6121 gcaatggtgg cggtcaacgt tcggacaggc tggagcaggt tataccacgg cttcggtatc
     6181 tgggccgcgt tgccggacgc catccacacc accatagtct cgcccatcgc gcgcatgatc
     6241 cccagcaaga ccgctgaaaa gattccgctg aacgccgccg ggatcaccac tttgagcaac
     6301 gtctcgctcc gggtcgcacc caacgcgtaa ctcccctcgc gcaactccct gcccgccgcc
     6361 tgcagggcat cttcagagac gctgacaatc gtcggcaacg ccatgatcgc gaggatcaca
     6421 gatgcgttca gcgcattaac gccactcgcg atttcgatgt tgcccggcaa ccactcgccc
     6481 gcattctgca aaaacggcgc cacgaccgtt aaagcgaaaa acccgtagac cacggaggga
     6541 atcgcggcca gaatctcgat aaccggcttg acgagctgcc gcgccttaaa cggcaggatg
     6601 tcgctgaggc agatggcagc catcacgccg gacggcacgg ccaagataac cgcagccgcc
     6661 gtcaccagaa aactgccgaa aaagatcgca gccgcgccga actgcggcgg cgtgtgactc
     6721 ggataccacg atttggagac aaaaaattcc agcgaggtcc gatcgcggaa aaagggcagg
     6781 gcatcccgcg cgatgaatag aatgatcgcg aacacggcca ccgccgccac tgacgtgacg
     6841 gcgaacagaa aggaacgccc taaaaaaccg atagtcttcc ggcgcattat tgcgccggaa
     6901 gacatctgaa gtgatccggt ggtagccctc atgtcctatg gaaccggaac gaacccgatc
     6961 tcctcgatga tttctttccc gcgggcggtt ttgctcaacc cgatgaattt cgcggccaac
     7021 gtgtccgccg ccggttcgcc gttggtgaac atgaaaagcg cccgcgagat cggatagcgt
     7081 ttctgcacca ccgattccgc gcacggcgcg acaccgttga tctccagcgc cttgaccgta
     7141 cggtccacga atccgagtcc ggcgtacccg atcgcgcccc gggtcatttg cacccgctga
     7201 cgcaccccgc cgttgctgcc ggtgtactcg gcactctcca agatcttgtt gtccttgccg
     7261 agaaccaagt ccttgaaggt ttcgtaggtg ccgctgttgg tgtcgcggct gatcaccaca
     7321 atacgctgat ccgcgccgcc cacctctttc cagttggtga tcttgccggt atagatatcc
     7381 cgcacctgct cgagggtcag gcccttcacc cggttggaag gatgcacgat caccggcaac
     7441 gcgtcaaatg cgaccgtgtg ttccacgggc ttgacgccct tttcttccat cgccttccgc
     7501 tccgcatcct tcagattccg cgagagattg gcgatgtcac atgtcccgtt caacagagcc
     7561 ttggccccgt tgccgctacc ggattcgctg accgtaatat tcacgcccgg attctgccgc
     7621 atataaaact ccgcgaacgc cttggcgatc ggtccgacag tcgtcgagcc gtccagcgtc
     7681 agcttatcct gagcctgcgc aaccgcgcaa caagccacca accctgccac cagtaatgaa
     7741 cccttcattt ttactcctgt gttgtgaaca gacaacctct ccgttcgatg tgagtgaacc
     7801 ctattcccgt tagacaaacg tgtcaccttt gttagaaaac cgttagaact tgccgttcag
     7861 ttcgaaccgc agccatgtcg ccacatcttc gttgaccgca tcgtgctggt agtagtcgcc
     7921 gtaccacaag gcctcaccga ccacagcgcc tttcacttcc ccgcgtttcc cgaagatctt
     7981 gctcagcagc gggaagtcgt atcgcgcctg cgtgtaaagc ccgcgatacg agcttgtcgc
     8041 gccgttgtcc ttctcgtgcg cgaacatcgg cccgcactgg actttgacct tgtgtttttg
     8101 gaacggctca acagcggctt caagatgcgg gtagatcagg ttggaccagc ggtactggtc
     8161 atacatgctc gagcacagct cgctgaacca ggtcgtgcgg ttgaaaaccg ggttccatcc
     8221 cgtgtccgtg ctgccgtcct gcgtcttgta gtaactatcc gtgtcaccgg acatccagag
     8281 caccgcgccg gtcagcttcg gtttgctgaa aacctctttc tccgagtagg tcaggcccgc
     8341 ataggtcatc cacgccaaga tgtcgcgtcc ccgcataccg gactgactgt caatctgccc
     8401 tgtctggaca gccgcttcga cctcagccga cagtttctca ttgatcttcg gcatcagacg
     8461 cgttccgagc gtgtgaaaat cacggccggg gtatcttgtt ttcttatcga aaaaccggtc
     8521 ttcctgtttc caaatccagt aaaactccat cgggaagttc tcgatttcgt tgtagtgcac
     8581 atacgccatc aagccctttt catccatctt ggagtacggg ctgccgcttt tgatcttggt
     8641 gttgtcatag acgtcatgcg gattgcccag agtccagtca tcgcgatacc ggttccaagt
     8701 ggccatcaaa tccacatcgc tcttctccag aaaacgcacc ttggcaaaga gcgcgtcaaa
     8761 gtacgtggag cgcgaaccgt cacccggcgt tccgtcgctg atcacccggc ccgcaccctc
     8821 ccggatatcc tgccgtccga cacgcagatc aacccggtcc gccagattgt tgaaatccag
     8881 atagaggtta tcgatgaaaa gctcgtcggg gaactcgttc ttccgtttat tccccgtgct
     8941 gttgcggtaa tcgcggaatt cgttggcgag ccgaagaaaa gctgcgtaat catcgccgaa
     9001 cgtagccttt ccccaaaacc gcgtccggag ccggtaataa tcctcatacg ccggattgac
     9061 ggatgtcttg cccttgttca tccagttgtc tttgaactcg taacgggcac gtatgtccat
     9121 tcccgcatcg aaatccagtt ctgccttttt ctccgttgct gccgccccgt cctgcgccaa
     9181 cacaggcatc gccaagaccg tcaaccctgc acataaccat cgttttcgca tgtctgctcc
     9241 ttttctgttc acgcagaggt gttgttttcg cctatttcct cttggaacgg cgtcatcatg
     9301 ccgaatgtta ataagatttc ggtgaggagt acatgagaaa tatatgttga aacgatatcg
     9361 gatcaaaatt gatttcggcg gtccggctcc gcgccgatgg cgcgcagctg tggacgtccc
     9421 gccgtaccgg catggatcgg aagggcaatg caaagtgcgg gcggccgtct cggacgcccg
     9481 aggatgcggg aacgggagcg gcgggcactt gatcgttcag tgaccccgcc gcccctgcgg
     9541 atacgcttat cccctgtcga ggcgccgcat cacggacgcc ggatgccttc gacccactcg
     9601 gccagtttca acatgccgcg ggtggcgtcg tagtagctga tcacgggacg cccgtccggc
     9661 agggaccggc ggcggtttac ggggaaaccg ccctaccacg ccgggtaggg acgcatcccc
     9721 gaggcgtccg cggacgatcc cgatagcgat tccgatcccg attccgcccc aacgcgggcg
     9781 gagcccgcac ctcctgacca cttgcggatg tcgagcatgt acgcgtcggc aagccgccgc
     9841 tggatcattc cgaacacgga ttttttgaga atttttaagg gttgactttt acccacccgc
     9901 acataacatg ataccaattc ataagggctg gcgggttact ggtttaccct atgggatgct
     9961 agtgataaaa tgtcgctttg atgagaaagt gtccatgacg aaagcaaact ggagatcgcc
    10021 gcccgtgatt ttgaccgcga gcatccgaat gggaggcttg ttttgatcga gacctcgaga
    10081 gattccgcgg ctgcgtttca ggagctgatc ggcaacagtc ggttcagttc cagtcatccg
    10141 ggaacgctgt cgattgaaaa cagcggcaag agccttgtct ataccgcacc aaagaaggtc
    10201 ggttccgtga tttacatcca ataagggatt acgatgaccg taaaggtcag tccgttatcg
    10261 ttttgtacag cgatcacgct gtccgtagtc ggagcagatg cggcaacgtg gtgaaactcc
    10321 aagtgggtac agcgatggaa aagggaaaga catgaaaaag tggatcatcg cggttgcgct
    10381 cgcggcggtg aatggctttg ccgggacgaa tcccgaacgg aaggtgtatg cccatttcat
    10441 ggggtgctgg ccggttttcg gttgcggagg ccaggacaag tccatgaagg cgtttctgac
    10501 gaccggcgag gccgtggaat gtggaccggt cggcggacgc ttcaacaatt tcccgctggt
    10561 gccgcaggag gggttcaaca tgaagctcct cgacaaggcg aagctggaga tcaaacgcgc
    10621 gatccgcggc ggtatcgacg gattcgcgtt cgatgcttgg gcaggcgacc agtccccgca
    10681 gacgctcgat gtctattttc aggcggctga agaactgaaa gtcgatttcg gactcaccat
    10741 ctgtttcgat ccctcctgcc atggcgcaaa gtggatcgaa ggcacgaata tgtgtgaaaa
    10801 gtacgccacg tccgcgaagt gggtgctccg tcacctcgat tccccgaacc tcgcgcgctt
    10861 ccacggcaaa ccgcttttct tcggctatta ttcaaagggc atcgtcaagg atagcggatc
    10921 cttcctgact gccgaaaagc gccgtgaaat caaggcagcc tgggacgcgt ggcgcaaggc
    10981 gctgccgtgc gaggtctatc tgcacggctc gatcgaagga tacgtcgact ggaaaaattt
    11041 cgagcgcaac gattatccgg cgatcgcaaa ggactgcgcc gagacctttg atgcggtcgg
    11101 cgccttcacc gggtgggacg cgaactgggc gaaccgcaac gatctctgga aacatatccg
    11161 ggacgctgga tgcggctgga gtcagccgat tgtgccgcaa tactgcaaca agaggggcgg
    11221 catcatcacg ggtgccgggc tcgatgttct gcaccagtgt tggacaaacg cgattgaccg
    11281 cggttcggaa ctgttgcagt ttgtgacttg gaacgactac ggcgaagaga cgatcctcgg
    11341 tcccgcgtat ggttcgaatt acacgtttct ccgcgtgaac cgccattacg ccgactggtt
    11401 gaaacagggg aagccgcccg ttatcgagaa ggatgaggtt catgttgttt ttcgccggtg
    11461 ctgcgatccg caaaaaacga cgttcccgtt ccttgatcgc cgtataaatc tgccgaccgc
    11521 gcttgaggtg atcaccttcc tgaccaagcc gggcaaggtc gttgtcgagg gctacggcaa
    11581 atacgaagcc ccggccggaa tgttctcgaa gaagttcgac gagcatcccg gaaacgtgtc
    11641 cgtgaaggtt cttcggcgca actggctttt cctcaatcag acggtctgtc aattgacggc
    11701 ccccgaggaa atcagtgcca agaagtggcg tgaagatgat gttgccgcca catggagttc
    11761 aacctatgac gaggagtggg cgttggattt tcccggcatc gaaccgctgt actattcgga
    11821 gaacggcgat ctcgacgggg atggcctgcc caactggttc gaaatggttt atttcgggga
    11881 gttcccgtat atgaagacgg cgtccggtgc ggatccgaat gccgatccgg acggtgacgg
    11941 gttcaccaac ctacaggagt acgagaacga gacgaacccg ctgattcccg atacccccta
    12001 tgcgtcagga tttgtgtggt cgcttcagga actcgcggag cgtgcgctca tcggtaatcc
    12061 tgcgagggat tcgaaggggc gctacgtgtg gcgacgcgag taccagtacg gtgcgaaagg
    12121 acacgactac gtgccgggca gtgcattcaa ggagattctc tcgggcgggg gctcgttgca
    12181 gtcacagatg acgcatgtct atgccccgaa tccgttcggc ggcggcggtt tcgactgcgg
    12241 gatccagttc aacaagcctg agaagggaca agccgaggca cgcgttcacc gcgattcacc
    12301 gattgcgctt acttggatcg cgcctgtcgc ctgcacggtc gatgtcgtga ccaaggtgac
    12361 gccgaagaat ccgaaggtaa aggcgaggac gacgctggtt ctgggagaca aggtgcttgg
    12421 gactacggtc gcgcgtaaga tccaggtcgg gaaaggggat gaaatccggc tcgtgatcga
    12481 gaacctcaat ccctggagtg gcggcgattt gttcgcgatc gagaagttcg atgtcatctg
    12541 ggcgaaggaa tcagaaaggg ctaattgaat tagcccatac ggcgattaag atcaagtgat
    12601 ccaggcggcg attctgcatg gagccagcga aaaaaacaaa cgcccggcca gatgtctggt
    12661 cgggcgtttg tagtaaaatg gctccggcgg ctggattcga accagcgacc aattggttaa
    12721 cagccaactg cgctaccgct gcgctacgcc ggaattgtcg ttcggcacaa ttatcccgca
    12781 agggaacaaa gtgcgcaaaa tgtatcggat tggcggaaag ttgtcaatgc gcaaagtgac
    12841 ccggttttgg tattgtggat tggttccgga cgcggtttgg gcccgtgccc cgccgatacc
    12901 ggaaacctgc cgagatcgct gccaaactca acaaaggggg ttgacctccc cttcccgttt
    12961 ccgcatactg gtcgcacgca atcgacgctt gaggagatta tgacgctgac aggcacattc
    13021 accgctttgg tcaccccttt taacacggat ggcagtgtgg attatcccgc gctgcacgcc
    13081 ttggtgcaat ggcagatcga gaacggggtc accggtctcg tgccggtcgg caccactggt
    13141 gaatcgccca ccctggagat cgatgagcac atccgtgtca tcaccgccac ggccgaggct
    13201 gctgcgggac gcgcagtgat cattgcgggc accggcgcca atgccacagc cgaggcgatc
    13261 gaactcacgc gccaagtgct gaacgcgggt gttcaggcta cgttgcaagt gacgccttac
    13321 tacaacaaac ccaacgccga ggggctctac cgccatttcg ccgcggtggc ggatctgggg
    13381 ctgccggtgg tgttgtacaa cgtgccgggc cgcgcgggac gcgagatccc gctggatgtg
    13441 gtgatccgcc tcgccgcgca tccgaatgtg gctgccatca aggaagccgc aggcagcgtc
    13501 gaccgcgtca gcgccatccg aaacgcctgc gagttgccgg tgctctcggg tgacgacagt
    13561 ctggcgctgc ccatgatcag cgtgggcgcg tgcggcgtga tcagcgtggc ctcgaatgtg
    13621 atcccgaagg agatgtcaaa gctcgtgcag ctcgccctcg ccaatgatct ggttgaagcg
    13681 cgcgcgctgc accagaccta ctatccgctt tttcgtgacc tgttcatcga taccaacccg
    13741 atcccggtca agaccgcgct gacgctgatg aatcgtgtgg ggccgacttt ccgtctgccg
    13801 ctgtgcgaac cgtcggaaaa gatccgtgcg caactcgtgc aaaccctgac ccacctcaac
    13861 ctgatgtaac ggcccgatcc ggccggcgtc attccccacc aaccagacga agggacgatc
    13921 ctgatatgaa gattgcgatt ctcggagccg caggccgcat gggacagaac ttgctgcgat
    13981 gcgcggaaaa gatcggtgcc tgccaaatcg ttgccgccac cgaatgcccc ggccacccgt
    14041 tgctgggcca atcggtgcgt caggccttcg gtctaacggc ggagaacatt ccagagaccc
    14101 tgcgcatcga ggaaacgtgg ccaaaggaca cggaagtggt aatcgatttc acgttccata
    14161 caacggtgac caacaacctc gcccgcgcga tcgcctgccg tcaggcgtac gtgctcggca
    14221 ctacggggtt tacggaagcc gagcggcaga cgatcctcgc cgccacacag caaatcccca
    14281 tggtttgggc acccaacatg agtctgggtg tgaacctgct gtttgaactg gtgcggcgcg
    14341 cggcgtcggt gctggggccg gagtacgacg cggagatcgt ggaaatccac caccgccgca
    14401 agaaggacgc gcccagcggc acggcactcg ggctcgccga ggcactggcc cggggccgca
    14461 acgtgaaact cgatgaggtc gcctgctacg gacgccaggg aattcccggc gaacgcccgg
    14521 aaggcgagat cgcgatccac gccctacgcg gcgggggcgt ggtcggcgac cacaccgtgc
    14581 tcttcatcgc cgacgaggaa cgcatcgaac tcacgcaccg ggcgaacagc cgggaatcct
    14641 tcgccgcagg agccctgcgc gccgccctct ggctcgaagg ccgtcccgca ggcctttaca
    14701 ccatgcggca tgtgctcgga ttcgaatccg tgtgacgccg cgttcccccc ccatggcaac
    14761 cccacgcgaa gccattctgt ctctcggcag caatctcggc gaccgccgca tcgcgttgga
    14821 gcgggcgtgc gcggcgctgg aagaggagcc gggcctccgg attctggagc gttccccgat
    14881 ctacgagacc gagccggtcg gcgtgccgga agaagaggcc ggaagacttt tcctcaatca
    14941 ggtggtgatc gtttcgactg accgttcccc cgaggcgctt tccgacacgg tgcatgcgat
    15001 cgaggcgcgc tggggccgga cacgaaccgg tgtgcacggc gcggcgcgcg tgctggacat
    15061 cgacctgatc gccttcggca cggagcgccg gaacacgccg gaactgacgc tgccgcaccc
    15121 gcgcacccgt gaacgccgct tcgtgctcca gccgctcgca gacctgaggc ccaatctcgt
    15181 cctgcccggc gagtcccgaa gcgttgcgga attactgcgc gatctacccg acacaccgcg
    15241 tgtgcggcgg atccaacaag cctaacgcag aacccaaccc gcaacccaaa aacgaaccgc
    15301 agagagacga aacctgctcc gcagaaagtc gaaggatccc gaccagaatg agaatggttg
    15361 ctgccgggcc cataccgggt tgcggcacgg gtagggcggt ttccccgtaa agagcagttg
    15421 aaccttcaac tctctttggt ttcaaggttt cgggttttca aggtttcatc caaatcgggg
    15481 tcggggtcgg gatcgctatc gggatcgatt gcttaaccta aaaagagcag ttgaaccttc
    15541 aactctcttt ggtttcaagg tttcatccaa atcggggtcg ggatcgctat cgggatcggg
    15601 atcgatcatt taaccacgga ataccaccgc ctcacagcct aacagcctca cagtctcaca
    15661 gcctttgaga ttgcgggcgc cgcccgcatc tcaatacgtc aaaacccaat gtttacgggg
    15721 ctttgctaga attctacccg ttcaactgct ccttttagga ttaacgaaac aacttcggcg
    15781 ttctgtataa gtctgcggat cagattgcct gatcgtttgt tcccgaacac gacatgaggc
    15841 tctgcggacg cctcggggat gcgtccctac cggctaacga acaacacacg acctgaggca
    15901 ggatcacacc agagaccacg gcccgcgcgg cgtacgtccg agcagggctt cggcggagac
    15961 gtcccccaga atctgttcgc ggaccggatc ccacttgatt ttccggccgg tgagcatggc
    16021 gatctgtcca agatgtccga tgctcgcgga acgatgcgcc acctcggcgg gcgtgagggt
    16081 ggtcgcgcgc gtcttcacgc aatcaatgaa gttgcgatga tgaccgggac tatgcaggcg
    16141 caactccccg ggctcgatgc ggctgtcgcg gatttcaggc gtggacgcgt caaaccggcc
    16201 gcgatccacc cagacccatt caccgttctc tccgtaccag gtggtgccgc ttttgacctg
    16261 cctgctgtcg gccaccgtca tttcgacccc gtcggcatag cggcaggaga aacgataggt
    16321 tttgggcgag tcgcaaatac cgtcgacctc gaacggcgcg tatccctcga acgtgaccgg
    16381 tcccgacgtg tcgcggtcca atccccactg cgcgatatcg gcgtggtgac cgatccagtc
    16441 catcatctgt cccccacccc agtccagtac ccagcgccaa tcccagtcgg cgatgccctg
    16501 atagggccgg tacggtgccg ggccgagcca gaaatcccaa tccaggcctt tgggaatcgt
    16561 cgcgtgcgga tcaatcgggt ttctggggcg tccgcccgga ggcagcccga cctccacccg
    16621 cgcgatcttg ccgatccgcc cgttgcgcac cagctccacc gcacgccgca tctcgcttga
    16681 cgaacgctgc cagcttccgg tttgccaaat gcgtccgtgc tgctccacgg ccctcaccag
    16741 tgcccgccct tcgcgcagat catgcgtgaa gggcttttcg ccgtagatgt ccttgccgtg
    16801 gcgggccgcg aaggttccca cataagcatg ccagtggtcg ggcgtggcaa tcatcaccgc
    16861 atcgatgtcg ccgcgggcac agacctcgcg gaaatccttg gtcaccaggc agtcactgtt
    16921 gccgtagtgc ttgtcaacct tctgtttggc ctgttccgtt tttgaggtgt tgacgtcgca
    16981 gaccatacgg acgatcacat ccttcatgcc cagaaagctg ctcatattgc cctgccccat
    17041 gccgccaacg ccgatcatgg ccatggcgat acggttgctg ggcgcgtcct ttccaagcac
    17101 ccgcgccggg atgatattcc atgcggcagc ggaagcagcg gccgtcttga gaaaacttct
    17161 gcgtgaaccc atcatgaggc acctcttttc ttttgcaaga tacgatgttt caaaattaac
    17221 gtaacgactc agcgacctgc cggatctctg cgactttctt ggggtcgaag cccgccatga
    17281 tctggatctc atgaatggac cagtgcttgc caggacgccc gccggtggtc acgatcttga
    17341 gatggcgccc ctgcgtcagc atcggaatga cgtttttttc tttctgtgtg ccgtccccct
    17401 tggccaccgg tccctgccac gttttcccat cgtccgagat caagacctca aagccgttcg
    17461 gcgtgtcgct ggctgatctt gtggtgtcga ggacaacctg cgacacacag agattttcac
    17521 cgaggtccag cgtgaaccac ataccctctt ccgaagcgcg attggacgac cagcgcgttt
    17581 cgggtttgcg gtcaaaggct ttcgccgcat cggcggcggc gtgcgaggca ttcgcccgcc
    17641 acagggaggg cgcgacctcg cgccaaggct ggtcggcacg ggacttcacc ttttcgtcat
    17701 agaaccgcag atagagacgc ttggcgaaag gaccgcaggc ggcatcacca aacagcgtct
    17761 gcaaaaccgg gaagaacccg agtccgtcac gcaggaacat cgcttcggcc acactctcac
    17821 gccccttgtc atcaggagcg gtccgcaaca attcgaggaa ccgtccttgc gtctccgcgc
    17881 cgccgtgtgc ggagacaagt ttgaccgcac cgcgcaaagc gagttggcgg agccgcgcat
    17941 ccggttgcgc gctcaccgcc tctttcaaca ccgggtaggg ccgcatgtcc gtccaatcgg
    18001 cgagtgcgcg caacgcgcct tcacgcacac cggcatccat ctccttcagg gccgttgaaa
    18061 gcggcgcgac aaaagcgggg tcgcgataaa cagccatcag ggcgataagc gcgttccgtg
    18121 ccggcacacc gagcgtcttc tccccccacg tgtccaacag gacaacggag cgggcagccg
    18181 gatcgacccg ttttcccgca gcctgaagcg ccgcttcggc ctgcacctgt tcctcggcct
    18241 gaaggtggcg cagttccgtg aacagcgtcg cgtacatctg gccgtcagcc agaaggccga
    18301 gcgttttcca cgcctctttc cggacctcgg cgcgctctga acggaggcag ggcctgatcc
    18361 gcggcaacag cgcaacctcg ccgcgttcgc ccacgagggt aagcagccgc gtctgcaacg
    18421 cgggatccct tgcggccacg tccagcagtg ccgcacccac cccgggcgcg cgcagcgcca
    18481 gcaacgcctc gcgggcggct ttgcccacca caccctcgcg tgtgatcagc gagaccatca
    18541 ccggaacctc tttgcagccg cccaacctgc ccaacgcatg caacgcggcc acaacgacga
    18601 tctcctgttc tccctccagt tttgccacca cttcgccgac gtatgaacgg gcatcccgct
    18661 cggccaactt ggcgagaagc gccacctgat ccgtaggcgc ggcggtgtgg agcgcggcaa
    18721 cgagtttgcg aagcggtacg cggtcagccg cgcgcaccac ggtctgtcgg acgtgcggac
    18781 agccgtccgc catcccttcg gcaaagaaac cgcagtcggc ctgcaacagc acacgggccg
    18841 cggcggcgcg taccgggaag ggcgtcccgg catgtgcgaa caacggacgc gccgcgcgtg
    18901 cggcggcacg cgtgccgccc tgttccgcaa gggtgccaag acaggccgcg agtgcggtca
    18961 ggcgcaccgg ctccaacgcg ggccgcacgg gcttttgcag gaccgcgagc gcggttttgt
    19021 cgccgatccg gcccagtccg gcgagtgcgg cggtggccac ccgctcctcg gtatccgcga
    19081 catagggcgc gatggccgcg gcctcgcggc gctgtgccaa ggcattgagc agcgcacagc
    19141 gcagctccgg tccggcaacc gggacggcca gcgacaacac gcgggaggag gcttcccccg
    19201 gggttgcggc aagttgcgcg agcgcccctt cgcgcgtttc cggaatcgcg aggcaggttg
    19261 ccagcacgtc gacgggcacc gtctcgcctg cggcggtcat cgcccgcagc acggcctgcc
    19321 gcccgtacgg cggcactccg ttctctttga gcagtccgat cagcaaagcg cggttggcgg
    19381 catcgacggc agcgtgtccg gtacaggtga ggatctcccg caaatcatgc gcgatctcag
    19441 ccacggtaag gtccgtggcg gcgcgcacgc gatccaaatc cgcagcgcgt aacccggagg
    19501 gcgtatcgtc cacccgaaag tcgccgacgg catactgcac accgtcgaga atgtggatca
    19561 acgtggcacg gtcaaggaag gcgcgcgcgt cgtggccgaa cgaggtgtag aaaacgcggc
    19621 cccggccata cggccgaatc caggaaaccg cgtagtcgtt gtcggcgcgg cgctgtcccg
    19681 tcgccgcggc ggtggccgca tgggaaaggt ccagcgagac aaggacgcgc aatttggcgc
    19741 ggttgtagaa gggcgactgc tgctggtaga tctcatcccc gaacaggaac ccctgaccgc
    19801 cgaaggcccg gttgagcgga tggtccggct catccaattt aaaggcccac atgccgccgt
    19861 cgccccaagg gtgaccccag aaacgaccgc cgaccatttc agccgcctgt tcggcgtggt
    19921 agaaattgtc ggcaccggca tggatcacgg ccaagccctt gccggagcgg acgaacgccg
    19981 tcaacgcaga ttcgagaaaa ccgtgctgcc gcgtgttcag agccgtggtg ttgttgagca
    20041 cgagcgcatc ataccgggcg agacgttcgg gcgtcagggc ctcgtactca cgcgagaaat
    20101 cggctgtgaa cgcgcgggtc tgggaggcgg cgatctctaa cgcgcgattg ccgtattcga
    20161 tcgcccggcc gtggacaaag ccctcgcagc gccaaaacac gaggacgcgg cgcgcccggg
    20221 atgaccgggc cgggacccac gtctccacca actcgccgat tcgttcggcg tcagcctgcg
    20281 gcacggggtg gagtgtctgg gcggatgcga cgagcgccgc gccgctgatg gcgtatatga
    20341 tgcagagcgt acgaaggata acgttacggt tatacatgag gagcctttcc gtaaaaggaa
    20401 tgaaatgatt gtggaaatat aatacgtggt tccgcatctg tccagtatcg cgagagattt
    20461 tgatccggta tcgcgccact cgtctaccgc aatcaggtcc ggaatgtctg acgctccgtg
    20521 cgtgttttga gatgcaggta gggttaacct aagaagagca gttgagggtt cacgtttcat
    20581 ccgcagattg acgccgataa gcgcagattt ttggggagcg agtgtctcca tccccgtgca
    20641 cggcgcggtt tttgtcactg tgctctaagt tgcgtttgca ccaaacagat gcggcgcgga
    20701 tcgcagccaa agccaatgtt tatgagacgc ggacagcgtg tcgtgggaat ttcaaaagcg
    20761 gaattagatg tcctccagcc cgcagtcgtg catcacctcg tacgctagtg tgtagccgtc
    20821 accgtccaca ccgcttcacg cgcctttctc cgccgcaaca atccttacag cgacggcgat
    20881 agcgccttta tgctgcctcc atccccggcg ggcgcgtcat ccggcaacgg cggcagattg
    20941 gggaaacgct cgcgcaggtc gatgcgctgt ccggggggga ttttatcgaa gtgcgccttg
    21001 atggggttgt aggtgttcgt cacccattcg ttgcctgtgt tggcataccg caccaacgtc
    21061 gcccgttgct tgctgttggc gtattcggaa atacggtcaa gcaaaaaacg gtctgcatcg
    21121 ttggcttcgt tagaactttc tacattctga acgattccaa gcaagaacga agcgatgttt
    21181 tcctttgttt ctggcgtcaa tgttttctct tctgccccga ccttttcaag gaattttttg
    21241 ttgagaaaat aacgcctcga cccctgcacg aactgggttt catatatctt ccgcatgaac
    21301 tcgaaaccct cctccacgtc cgcgattttg acatacgctt ccgcagcacg tgagcggaca
    21361 aaaacgggag cgttcgtcat gaagcttttc tcctccagaa acgggagggc agcccggtcg
    21421 cccagttttt caatcatgcc gaacagtctg gaaaaatacg gcccgtcccc cccccagttt
    21481 ttagacgagg ggtgctccag atcgtaggcg atcacaacct tttctcgctg gttgaaccgc
    21541 tcgatcaaaa ggtcgagggt ccagcccttc gcgagcacct cttcgcgtcc cccgcaaagc
    21601 ccgccccacg gaaatgcgag ggaaagcagg agaagccgga taaaaccgca gattctgtgt
    21661 gtacacaatg tcatgtctga cctcctaaca cgggcgaagc ccgcgaccct caccccggcc
    21721 ctctcccaga gggcgagggt gaaaacaact tgttttttat tacgcttgtt gagtcgtaag
    21781 acactaatgg cttccggggt tccgctggat ggcgctcagt ccgaccccgg cattgcccag
    21841 cgtcctgacg ttgccgcccc cggcatgccg ccacccgtag acggtgccgc gcggcaggca
    21901 tatgccctcc gaaaccgggg gttcaggacc gaaggcgccc ccgcgcatga gaagccgcgt
    21961 gatcaggctc cgctgcgtca gccccggcgg atagtagccc ccgcaccagt cggcgggaat
    22021 gcgctcctcg gagactggcc cgggaaccgg gttgggatcc ggcccgtagg gattctcgac
    22081 cgtgtagatg tcctccagcc cgcaactgtg catcacctca tgcgcgatgg tgtggccgtt
    22141 gccgttgcgt gcgatggcga tgccctccgg ccagcacacc ccggcggcgt cctcgccttc
    22201 caacatctct acaaagtaga gttccaccgc gttgcccatg ctgttggtgg cattcagcat
    22261 cgcggcgagg ttggtgttgg ccgggttgag cacgtccgtg tggttcaacc atgcggcatt
    22321 gttcaaatag tgcaccgctc cgctctggac cagcgtcaac ccgctcggcc acaggatcct
    22381 gttggcgtcc gccagaagac cgggaatctc ggcggcttgg cgggcggggt ttttaccgtc
    22441 atcgtctctc acgatccaca cggtgacggg cacgttgacg gcgggacgga cggtgccggt
    22501 gaagaagggc tggacgtggg gcggcgtgat gaagaggttt ttgatgttcg cctccagccg
    22561 aaagtcgccg gtcgcggtcg cgtgcacggc gacctccggg ccggtattgt cgccgacgaa
    22621 ggcgacgcat cccgcgccct ccgtgaccgt ccaagtgatg tcggcgtcgg gcacgttagc
    22681 ggacaccccg atcttgaagc ggccggtgcc gcccagcggc aggccgctgg ggttgaacac
    22741 gtgcccggcg gcggtgacat ccgcgccgaa cgcggcgagc gccagaaaaa cggcggcgct
    22801 ccggaacatg gcgaatcctc tcattgacag actccttttc tccctctttc cctctcccct
    22861 gcggaaaggg cgcgtccccg cgcaccccgc ttctccaata aaacggccgg tcttccggct
    22921 gaagcgtcat gcgcgaaagc gcaaaatcag ggtgccgggg gttccggccc ctgtttcatg
    22981 cgttcgttga tgcagcgggg cagaacgtcc gccgtgaacg atctgcgtgg atcgtacctt
    23041 tttcaggcaa tgatcgcaac cggtttttga gcaaatgtgc ccgcttttgc cccgccccca
    23101 acaggcaagc ggggaagaca gaaactgtaa cgcgggcagc gttaacgaca taaagcgaaa
    23161 accgtatcat ggtggcgtat ttgggactaa gtgatacaca accgatgttt agttttgttg
    23221 tatgcaaaca aggtgacaaa aaacgcgaac cgtgaacgca ggcctgcgtg cagtcattgc
    23281 gcgatgcaat cccacgcgaa ggaactggaa cgtttgcgac gtttgtctgt tgaggatagg
    23341 atccgttcag ctttgacgat gaaccaacgg tttgcgtgga ttcgcccggt tgcttcggag
    23401 caagccccat gagtgacttg gatgaggtgc gccagacttg cgcccgctat caactgactg
    23461 gacttgacga gttgatcgcg gagtccggaa tctaggagtt ttgcatcatc gtacggatac
    23521 ggacgcggcg ttacggcgcg agcggtttgc cgaacaggtt gccggtcagc gttcccaaga
    23581 acgcggtgag cgcgtccaac tcgtcttctg acgcgacagc gccgacctga tatttcatca
    23641 tcgagcgcac cgtctctt
//
LOCUS       DAOSLE010000024        23136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2446_length_23136_cov_8.377843, whole genome shotgun sequence.
ACCESSION   DAOSLE010000024 DAOSLE010000000
VERSION     DAOSLE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 23136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23136
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2446_length_23136_cov_8.377843"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK576_03150"
     CDS             complement(<1..317)
                     /locus_tag="PK576_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60617.1"
                     /translation="MTKRTILVGMIALTGTAVCASAEESPLSGVDTWGYRKALPAVVN
                     PAVRSPLQSVVSLRGEWKFVAQPTNVRPRRHPGWDRFFNRPWQNARTIQVPGCWEAQG
                     VGEP"
     gene            complement(314..532)
                     /locus_tag="PK576_03155"
     CDS             complement(314..532)
                     /locus_tag="PK576_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60618.1"
                     /translation="MTTPVMSEETRLVVQAMDEATWKAIEGYRQTGLPVPCWRDGKVV
                     YLTVDEALASRSDYQERMGKPTPSEEKQ"
     gene            complement(529..1203)
                     /locus_tag="PK576_03160"
     CDS             complement(529..1203)
                     /locus_tag="PK576_03160"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOE60619.1"
                     /translation="MIDAILSDIGVPECLLPTDPSTRAVRESLGDSRLFDSPACHIVA
                     GANGSGKSTFALHFLPRYAECLEFVNPDLIALGLSPFDATRAAVKAGRLVLERIASLS
                     AAKKDFGFETTLSGLGYLELLKNLRSTGYRLHLYYLWTPSCELLLSRIRQRIATGGHA
                     VPDQDVRRRYDRSLTNLKAYAAVMDKVRIFDNSGMKPVLVYEKNSEALVYDTVRFAQM
                     KLEVAL"
     gene            complement(1390..3252)
                     /locus_tag="PK576_03165"
     CDS             complement(1390..3252)
                     /locus_tag="PK576_03165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOE60620.1"
                     /translation="MSGLWTEDRLKQELSDRFGYAAFRAGQGEAVRTVLDGRDLVLVM
                     PTGSGKSLCYQLAALLLPGVTVVVSPLIALMKDQVDALDRKGIPATFLNSSVDSAEMA
                     YRLDGLRQGRYKLLYVAPERFRNRRFTEAFAAVQVALFTVDEAHCISQWGHDFRPDYL
                     NLKHVVERLGAVRVLAVTATATPDVREDIVRQLGLGVAPRQAPEVRVTGFARPNLHLA
                     VTRCATHRHKFTRLVQMVEAHRCGIVYCSTRKMAERVAARLESEGLAPITYHGAMADG
                     ERTAAQNRFISEPSPVVVATNAFGMGVDRSDLRFVTHWDIPGSLEAYYQEVGRAGRDG
                     ADAWCELLFNYADVGTQRFFLDGANPPFDDVLAVWQTVRKACESAPVTWSVERWAGEA
                     GLGNEMAARTILGLIERARLIEREILPGQRTYTTRLLPNPDPALKELKRLTAGLEEKR
                     RRDERKLETLLRYVDHPGCRHAFMLNYFGDQTSSSSCSACDRCQYRAPHPPTELTEEQ
                     WIEVQKVLSCVTRMKGRFGAQRIAQVLRGDSNEVLTRHALDQLSTYGLLPDKSVAQIR
                     AILDALHGAECVAVTPDTYRLVSITPKGHEVMMRRLKGFRLAWPQKGQRSRRYY"
     gene            complement(3277..4290)
                     /locus_tag="PK576_03170"
     CDS             complement(3277..4290)
                     /locus_tag="PK576_03170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOE60621.1"
                     /translation="MQNIDWSNLGFKYMDTCCHIRYVWRQGAWDEGELVSDPWLKMHI
                     AASCLHYGQAAFEGLKAFHCQDGRVRIFRPEANAERMANTARRTCMAQVPVAMFTEAV
                     RRVVKANEDYVPPFGTGGSLYIRPLLIGSGAQIGVAPADEYTFLVMVMPVGPYYKGGL
                     KPVRALILDDYDRAAPQGMGDVKVAGNYAASLYAHESAKHAGYPVELYLDAKTHTLVE
                     EFATSNFIGIKPDGTYVTPDSCSILPSVTNNTLQQIAKDLGMSVEARAVPYDELPEFS
                     EIAACGTAVVITPVYEITRGDRVIRVGAPDGCGPVLQKLYDTVQGIQYGVLPDRHGWC
                     VEV"
     gene            4485..5540
                     /gene="argC"
                     /locus_tag="PK576_03175"
     CDS             4485..5540
                     /gene="argC"
                     /locus_tag="PK576_03175"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOE60622.1"
                     /translation="MIRVKVVGATGYGGVGITELVLQHPKAKLVSLIAREAVGQPLSA
                     VYPHLAGFCDLPVLAAEDPRAQEPADVVFFATPDRVGMCGAPEELARGAKVIDYSGDF
                     RFTSAEAYADYATRIGKDPVHAAPELLGRTVYGLAELHRDAIRTSTLVGNPGCFACSC
                     ILGMAPAVRERLLMPDTIICDCKTGVSGAGKKVNAAHHYPARYEQMNAYKLAGHQHVC
                     EVERELGLLAGQEVRITFTAQVVPVCRGILSTLYGTLTHAMPEAELVALYRDFFRESP
                     FVRVFDSQTAIGTLNVRGTNFCNLVVSVDARVNRLRVVAHIDNLMKGQAGSALQNMNL
                     MFGLPETTGLDRPGIYP"
     gene            5537..6577
                     /locus_tag="PK576_03180"
     CDS             5537..6577
                     /locus_tag="PK576_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE60623.1"
                     /translation="MSNKKSAPKKVVAAAGRKTAPAASRKHPKPGLGRGLGALISRPS
                     GNAVQTPNVAARMDAPERTGYDTAAPDPRRSVLDVPVAEISRSPWQPRQTFDAESLSE
                     LADSIRANGVIQPLICRKTGDARYELVAGERRLRAAIEAKLATVPVILVEAEDRRAAE
                     LAIIENIQRQDLNAIEEAEGYHTLAETFNLTQQEVADRVGKARASVANALRLLDLPDE
                     VKQLISSGLLSTGHAKVLLSLTDVTEQTLLGRKAVTEGLTVRALEKVVQRRNKRDAGA
                     TLRRPGQPDLPESYVRDLTDRLHKHFGTAIRLSSSVTHANGKHTKGILEIDFYDNDDL
                     DRLLTLFGITLD"
     gene            6606..7544
                     /locus_tag="PK576_03185"
     CDS             6606..7544
                     /locus_tag="PK576_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOE60624.1"
                     /translation="MRRMMLGWVLLGLTGCDLGTTNPEPVSAPPPQVWTAARASNAFA
                     YVEGLVRDCTPRDAGTPGGEKAACWIRDRLAEQGLPARIDRFTEPTPRGPREFRNVLA
                     TLPGTGDTWIILLSHFDTMSGIGEGFQGANDSGSSTGLLLELAGALRDAGPREPSILF
                     GFMDGEECMIAYGAQDGFHGSKRLARQMKEEKRKIRAVILTDMVGDRDLKLTLPRNST
                     AALRVLALEAAEAVGLRDHIGLFDGNIYDDHQAFLDLGYPAVNLIDFSYGSRPGLNDY
                     WHTRQDTLDKLSVESLRITGAIVLEMVNRLSSPARR"
     gene            complement(7566..8612)
                     /locus_tag="PK576_03190"
     CDS             complement(7566..8612)
                     /locus_tag="PK576_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE60625.1"
                     /translation="MDGQFDANADTTMEVRFGDTPPEPMPNRIDVARDPAVGQFVRYL
                     TAERNASAHTVAGYLQDIGQYAAFVWGTEKRGGPPFAWDEPDRARARAFLSAFHRKGH
                     APATTRRKLSSLRTFYRYLEREGRTTAQPFAGLRGPRLGKRLPVVLSLREVEALLAAP
                     LAALRKRSDAGRAPSLWAEYAALRDTAIFEVLYSTGCRIGEVAALVWRDIRFESGTTI
                     VEGKGRKQRLCVLGRPACDALIRLRDAATRLWPATAGPQAPLFLNREGGALTSRSIER
                     QMKVWLRAAGLPQELTPHKLRHSFATHLLNAGADMRSVQEMLGHASLSTTQIYTHVSI
                     DRLKDAYAKAHPRA"
     gene            complement(8659..11226)
                     /gene="topA"
                     /locus_tag="PK576_03195"
     CDS             complement(8659..11226)
                     /gene="topA"
                     /locus_tag="PK576_03195"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOE60626.1"
                     /translation="MSKKLVIVESPAKAKTIGKMLGSGYVVKSSVGHIRDLPERTLGV
                     DIEQGFVPKYVLSKGKTKVIAELKKAVKGCDEIYLAPDPDREGEAIAWHLRETLAEVA
                     DGKPFHRVMYNEITPRAVRAAFEKPGHLDMRRVDAQQARRVLDRIVGYMVSPLLWRRL
                     KRGLSAGRVQSVALRLLSERERQIQAFRPEEYWVMGARVRRALAPLDPFTVKLAKVDG
                     EKPVIGSETEARALLSDLEGCTLRVREVRVRETTRRPLPPFITSTLQQAASTVCGFSP
                     SRTMSLAQKLYEGVALDAGGPVGLITYMRTDSVNVSRDAQAAAREHIITAYGKEYYPA
                     SPNVYRSRASAQEAHEAIRPTEVTRTPESLKGILDASSLKLYDLIWRRFVASQMAAAK
                     IAQKTVEIEPVPPASTGVAPRRACLFTASASEVRFDGFLKVMALDIRRKKPEEEDPEE
                     EVEEVQRLPPLAKGDGLVPLEWLSERKETKPPARYSEASLIRALEANGVGRPSTYASI
                     LETLSSRDYTTREKRQLMPTPLGLEVNDLLVGKLGSLFDVGFTARMEERLDEIEAGGI
                     GWTAMLDDFYKRFEQWMEQAKEPPADAGKVETMLSLLERVTVWAPAVQRGNSKRVYSD
                     ERFVKSVREQIEKSARPVSEKQLTALAKIAVRYREQLPEVEAVLKSCGFGEIVETDNA
                     APSGEEVMRRFAVLKEVELSERQMKFVESLRHQAESGRKLSEKQMAALDRIVVQNAAQ
                     IRDFETVKASLGLTAEESEPSTDHESPVLLEMLGHVTEWQAPVQRGKMSFDDQAFFVS
                     LSEQFNHKRSLSPRQRYAMRRMVFRYKAQIPGFERYAAKLGLKSKSDKTETSGTKEA"
     gene            complement(11306..12442)
                     /gene="dprA"
                     /locus_tag="PK576_03200"
     CDS             complement(11306..12442)
                     /gene="dprA"
                     /locus_tag="PK576_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE60627.1"
                     /translation="MTEREALIALNQISGLGAVTTRRMAERFGDLSAVFGASESELLS
                     VPGIGAERARQFHAELRRVAAGPELARAEKKGVRLVTWADPEYPALLKQIADPPLVLY
                     VAGAAEALNQPAVAVIGTRRPTVYGRETARFFGYRLAAAGYVVVSGLALGIDTEAHTG
                     AVKAKGRTVAVLGGALDRLFPKENTGLAREIVAQGGAVISEYPFGRQPDRQTFPMRNR
                     IVSGLCKGVLVIEAPLNSGTLITVGQALDQNRTVMAVPGRIDSPVSQGCHRLLREGAR
                     LVTTADEVIEEVEDLLAGAKTRETPAAVRSGTEEARPPACVLSEDERKVVAQIGMEEV
                     SVDAVVRGSGLDAGTVNAMLVGLQIKRVVRLLPGGFVTRVSRAS"
     gene            12735..14378
                     /locus_tag="PK576_03205"
     CDS             12735..14378
                     /locus_tag="PK576_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60628.1"
                     /translation="MRSCLKARKPVAWFAMAALSVYGGPTSIPIRQGGVEGRPFWNKV
                     SPWFEYAPAFDFPEVNGAVKYRFIAKHQSGAEFSFEAESPQAALSPIWEKLPVGAIEL
                     RVAALDANGGFIETAPGNATVTGSAVAMKPPTAVRKFWKKATFHEGMYESAACSYAQS
                     ARKAFEFVLNEPSIRYLHDHGTPDPSYSLNGYPSKMLSAEIGILLDIAKTRPEHRAFA
                     LETAKKAADHLIRNRCAAGTPLEYFTKTYEGEGEYGRSKQNAGLNMLIYPAKAGEALV
                     KLHVALARDEGYLDAAEKIAATYLKLQGTDGTWPLKVREDTGEPVAPNRLIPLPVIDF
                     LESLYAVTKKPEYRAAANRAFAYVEKGPLKTWNWEGQFEDTKPRPPYLNPTMHEAAST
                     AIYLLKRFPGDAKRLELAMELVRWAEDQFVAWEKPEVGDRRAHHFNPANWYVPGTIEQ
                     LAMGEPIDASSAKMIEVYLALHKATGGHEYWAKARVLGDTITRAQWEDGCYKTFWVKA
                     DRDDQRYHVWMNCHIYTARVMNLLATVEHWLNGKPKRSS"
     gene            14774..16003
                     /locus_tag="PK576_03210"
     CDS             14774..16003
                     /locus_tag="PK576_03210"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE60629.1"
                     /translation="MSDHMPMATRVKLSAMMFLQFMMLPVWFIPMFPYITEMAPKGSA
                     LPLLCGMIMGFGALASPIFGMFADRLMNSEKVLALCNFVTAVLLAYAYTIKTPGMLFV
                     TLLLVMVFYMPTWSLTATIAMANSTTEAFPQIRVFGTLGWVAAAIFSIIGTKVFGIAQ
                     FDSTPYIFLGGAIAAALGGVLAFFLPPTQPPAKGQKMSLVDTLGLRALVLLKRPDFLV
                     FSVLILLAMVPFQWYNVYNSTYLKERGYEYLTGVMNLGQVLELVFMLLIPVILKKAGY
                     KWAMVLGLLALVFRYGMFYLGAAHGLQICDYAGILIHGLIFGMLVVGSQMYVDAAAPA
                     ELRGQAQGFIGLIMFSLGTFLSNFVFDGILKKNVAADGTHVWTTPYLIAMVSALVLAV
                     LMAVAFKPAVQEKRQGA"
     gene            complement(16045..16473)
                     /gene="dut"
                     /locus_tag="PK576_03215"
     CDS             complement(16045..16473)
                     /gene="dut"
                     /locus_tag="PK576_03215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009546472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOE60630.1"
                     /translation="MTVEFMRIHPEAVVPSYAHPGDAGMDVSACESAVLQPGERKRIR
                     TGLVVALPPGTEAQMRPRSGLALRHGVTLLNTPGTVDEGYRGEIGVILINHGDTPFEI
                     TAGMRIAQMVIAPVARAEVREVAELGGTVRGTAGFGSTGV"
     gene            complement(16663..17040)
                     /locus_tag="PK576_03220"
     CDS             complement(16663..17040)
                     /locus_tag="PK576_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE60631.1"
                     /translation="MSLMKRDRVEAEKANRLELTPVSRITESEEAFTLTMELPGVSEK
                     QVELTLEDRTLTVTAEKEVASFKDYTLILNEIQEARYRAAFELPERVDTAKVQASCKN
                     GMLVLTLPKREEVKPRRIAITAG"
     gene            complement(17069..17470)
                     /locus_tag="PK576_03225"
     CDS             complement(17069..17470)
                     /locus_tag="PK576_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE60632.1"
                     /translation="MRAYLSPWGNPWRVFDELMGDFPYDLFNSAWRARVGANPRVNVW
                     ESEKGLVVEAEVPGMEPDTLDVAVDANVLTLKGKRELPDGTENEFQRSFNLPFELDEK
                     GIKATVKNGVLTVTIPRKAAAEKRKIAIEKL"
     gene            17875..19989
                     /locus_tag="PK576_03230"
     CDS             17875..19989
                     /locus_tag="PK576_03230"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOE60633.1"
                     /translation="MSRYEQPDLILRIRKRDGRSEVFDSNKIVGAILRAGEATGEFAG
                     DEARKLTLKVMSIIHSLTDTLDLSVENVQDIVEEVLLASPYRRTAKAYIIYRDQHARI
                     REMLRKADVDLIDRYLGKADWRVRENSNMAYSLQGLNNYISSEVSKAYWLNKIYPAEI
                     REAHDNGALHLHDLNLLSVYCVGWDLQDLLRSGFKGVAGKAESLPAKHFRTALGQIVN
                     FFYTLQGEAAGAQAFSSFDTLLAPFVRYDRLNAAEVRQALQEFIFNVNVPTRVGFQTP
                     FTNLTMDLIAPSTLANEHAVIGGKEMPETYADFQHEMDMINTAFLEVMTEGDAKGRVF
                     TFPIPTYNITQDFDWERPGLEKLWEVTAKYGIPYFANFVNSDMSPDDARSMCCRLRLD
                     TRELNRRGGGLFGANPQTGSIGVVTINMPRLGYLATDEDDFLARLDNLMGIARNSLEI
                     KRKVLEQFTAQNLYPYTRFYLRQVYQRFGTYWQNHFSTIGLVGLNEACLNLFGEDIGS
                     GRGRAFALTVMDFMRARLADYQEETGNLYNLEATPAEGTTYRLARLDRKLYPEIITAG
                     GEGEPFYSNSTQLPVNYSDDILRVLDLQDEIQTKYTGGTVVHAFIGEAVSDPQAVKAF
                     VRMVCRNYRLPYFTLTPTFSICEDHGYLSGEVHECPHCGQYTEVYSRVVGYIRPIQQW
                     NAGKQNEFHQRKTYHVCEAAAR"
     gene            19986..20681
                     /locus_tag="PK576_03235"
     CDS             19986..20681
                     /locus_tag="PK576_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOE60634.1"
                     /translation="MNIAGVVPLSLCDYPGRVAAVVFTQGCNFRCPFCHNAHLIPLLP
                     VAGQMLEEEQVFARITEYRTRLDGVVVSGGEPTLQQDLPEFLLRLKTLGLAVKLDTNG
                     SQPEMLRRVLDERLVDFIAMDVKAPWEHYPRLTGTACAQDDLLRSLRLIAGSGLPHQF
                     RTTRVDPLLTDSDYRNVRAQIPDGSPHVWQAFRPEHAFDPALRPPRPCTDNVPVSVSS
                     RLSDCPQARERAQ"
     gene            20686..21537
                     /locus_tag="PK576_03240"
     CDS             20686..21537
                     /locus_tag="PK576_03240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOE60635.1"
                     /translation="MFVPNADNITARWVCQIAGGGILVAGVAAALALLWSAQRRRAAG
                     ARTVAVAHLASLPLSDVHAGVTLIITFLFALASLPRTAGTAFRPDDRALVLGPLLYLC
                     SSVLVVMFCGVHARCGLRRMFLAGPVRPAAAVAKGLVYGLAAIPPVALLSFLISAGLE
                     AWGFAPQHQEVFDWLGDASRAPGARLFMMGAAVFLAPLSEETLFRGILFPCLLRGRSV
                     VGAALVSSLYFATVHLHLASFLPLVVLGMFFSAGYAATGSLLTPITMHALFNFTSLIF
                     YLADPLP"
     gene            21581..22936
                     /locus_tag="PK576_03245"
     CDS             21581..22936
                     /locus_tag="PK576_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOE60636.1"
                     /translation="MTDQDLFDKSASSPEFPTGHAAEGLRPPLAARMRPETLEDVVGQ
                     EHILGPGKLLRRVIEADRLTNLIFYGPPGCGKTTLAEVISRITRRRFERTSGVLSNVA
                     TLRSLCETARLYRNDPGTILFIDEIHRFSKSQQDVLLPYVENGTVTLIGATTHNPNIF
                     VNSPLTSRSLVFELRPLSEEAITCLVQRALADSSRGLGHLPAELTPEATQFLATMCDG
                     DARRALNALEIAVRSTPPDPDGVIRITHAVIEDSVQRKMVVYDKDEDGHYDTISAFIK
                     SVRGSDPHAAIYWLAKMLEAGEDPRFIARRLIILASEDIGNADPRGLTVAVAAMHAVE
                     FIGMPEARISLAQATTYLATAPKSNASYLAVEEALADVRSGRVLAVPEHLKNVHVRAV
                     GREGLDTAYKYPHDYAQHTVDQLYLPKDKRYYRPAAQGYEETLARRMEHVERLKRETR
                     E"
BASE COUNT         4309 a         7569 c         6825 g         4433 t
ORIGIN      
        1 ggttcgccca cgccctgcgc ctcccaacat ccgggcactt gaatggttct ggcattctgc
       61 cacggccggt tgaaaaagcg gtcccatccc ggatgacggc gcgggcggac attggtgggt
      121 tgcgcgacga acttccactc gccgcgtaac gacaccacgc tctgcagcgg cgaccgcaca
      181 gccggattca ccaccgccgg aagtgccttg cgatagcccc atgtgtccac gcccgagaga
      241 gggctttctt cggcggacgc gcaaaccgct gtccccgtca atgcgatcat tccgaccaga
      301 atggttcttt ttgtcattgt ttctcctccg aaggcgtcgg cttgcccatg cgttcttgat
      361 agtcggaacg gctggcgagc gcttcatcca cggtgaggta aaccaccttg ccgtcgcgcc
      421 agcacggcac ggggaggccg gtctggcggt atccctcgat cgctttccag gttgcctcgt
      481 ccatcgcctg cacgaccagc cgggtttctt ctgacatcac gggtgtcgtc atagggcaac
      541 ctccagtttc atttgggcga agcgcacggt atcgtacacg agagcttcac tgtttttttc
      601 atagaccaag acaggcttca ttcccgaatt atcgaagatg cggactttgt ccatgacggc
      661 ggcataggcc tttagattcg tcagcgatcg atcataccgc cgcctcacgt cctgatcggg
      721 aaccgcgtgt cctcccgtcg caatgcgttg tcggatacgg gagaggagca actcgcaact
      781 gggggtccag agatagtaca ggtgcagccg gtaaccggtt gagcgcaggt ttttcagcaa
      841 ttccaaatag ccgaggccgg agagtgtggt ctcaaatccg aaatcctttt tcgctgccga
      901 cagggaggcg atccgttcca gaacaagacg gcctgccttg acagcagccc gtgtcgcatc
      961 aaaaggggag agacccaatg cgatcaggtc ggggttgaca aactccagac actccgcgta
     1021 acgcggtaaa aaatgcaatg cgaatgtgga ttttcctgag ccgttcgcac cggcgacgat
     1081 atggcaggca gggctgtcaa atagacgcga gtcaccgaga gactcccgca ctgctcgtgt
     1141 tgacggatca gtcggcagca ggcattccgg cacgccgata tccgagagga ttgcatcaat
     1201 caacgcgcct cggtccaccg gttcgttatc tgtccctgca acagtgacag tgtacggatg
     1261 ggggcacctt atgttctcgc ccgttttcat aacacacttc caatgtcgaa agtggcacaa
     1321 ggttacacgt tttcaggcct ttttgccagt gctttaactc ctaactccta aatcctaaat
     1381 ctcctaactt cagtagtacc gtcgcgaacg ctgccccttc tgcggccagg cgagacggaa
     1441 ccctttaaga cggcgcatca tcacttcgtg tcccttgggc gtgatggaaa ccagccggta
     1501 ggtgtcgggc gtgaccgcca cgcactctgc accatgcagg gcgtcgagta ttgcgcggat
     1561 ttgtgccacg ctcttatcgg gcaacagacc gtaagtcgag agctggtcta gcgcgtgccg
     1621 cgtcaacacc tcgttgctgt cgccgcgcag cacctgcgcg atgcgctgtg cgccgaagcg
     1681 tcccttcatg cgggtcacgc aactcagaac cttctggacc tcgatccatt gctcttcggt
     1741 caactcggtc ggcggatgcg gcgcacggta ttggcagcgg tcgcaggcgg aacaggatga
     1801 agatgaagtc tggtccccga aatagttgag catgaaggca tggcggcaac cgggatgatc
     1861 cacataccga agcagggtct caagtttgcg ctcgtcgcgc cgccgtttct cctccaatcc
     1921 cgcagtaaga cgtttgagtt ccttgagcgc gggatcggga ttcggcagca gacgcgtcgt
     1981 atatgtgcgc tggcccggca ggatttcccg ttcgatcagg cgcgcgcgtt cgatcagccc
     2041 cagaatcgtg cgcgccgcca tctcgttgcc aaggccggcc tcaccggccc agcgttcaac
     2101 cgaccaggtc accggagcgg attcacaggc cttgcgcacc gtctgccaga ccgcaagcac
     2161 atcgtcgaac ggagggttgg ccccatcaag gaagaagcgc tgcgtgccca catcggcgta
     2221 gttgaaaagc aattcgcacc acgcgtccgc gccgtcgcgt ccggcacgcc ccacctcttg
     2281 gtaatacgct tccagactgc cgggaatgtc ccaatgcgtc acaaagcgga ggtcactgcg
     2341 gtccacgccc atgccgaacg cgttggtggc caccaccacc ggcgacggct ccgagatgaa
     2401 ccggttctgc gcggcggtgc gctcgccgtc cgccatcgcg ccgtggtagg tgatcggcgc
     2461 aaggccctcg ctttcaagac gtgcggccac gcgttcggcc atcttgcggg tggaacagta
     2521 cacaatcccg cagcggtggg cttccaccat ttgaacaagc cgggtaaact tgtgccggtg
     2581 cgtcgcgcag cgcgtcaccg ccagatgcag gttggggcgc gcgaacccgg tcacccgcac
     2641 ttccggggcc tgccgcggcg ccacgcccaa gccgagttgg cgcacgatgt cctcacgcac
     2701 atcgggcgtg gccgtggcgg tcacggccag cacgcgcacc gcgcccaacc tttccaccac
     2761 atgtttgagg ttgaggtagt cgggccggaa atcatgtccc cactggctga tgcagtgcgc
     2821 ctcgtccaca gtaaacagcg caacctgcac agcggcgaag gcctcggtga agcggcggtt
     2881 gcgaaaccgt tccggcgcca catacagaag cttatagcgc ccctgacgca acccgtccag
     2941 ccggtaggcc atctctgcgc tgtcgaccga tgagttgagg aaggtcgcgg ggatcccctt
     3001 gcgatcgagc gcatcgacct gatccttcat cagcgcaatc agcggcgaga cgacgaccgt
     3061 cacccccggc aacagcaggg cggccaactg atagcagagc gatttgcccg agcccgtggg
     3121 catgaccagc acgagatcgc ggccgtcgag aacggtgcgc accgcctcgc cctgtcccgc
     3181 gcggaaggcc gcgtacccaa accggtcgga cagttcctgc ttcagccgat cctctgtcca
     3241 gagaccgctc atgggtgtcc accctctgcc cgcaggtcaa acctcgacac accagccgtg
     3301 ccgatcgggc agcaccccgt actggatccc ctgcaccgtg tcgtacagtt tctgcagcac
     3361 gggtccgcac ccgtcgggcg cgcccacccg gatcacccgg tcgccgcgtg tgatctcgta
     3421 caccggcgtg atcaccactg cggtgccgca ggcggcgatc tcggaaaact ccggcaactc
     3481 atcatacggc acggcacgcg cctccacact catgccaagg tcctttgcga tctgctgcaa
     3541 cgtgttgttg gtgacactcg gcaggatcga acacgaatcc ggcgtcacat acgtgccgtc
     3601 cggtttgatg ccgatgaaat tggaggtggc gaactcctcg accagcgtgt gggtcttcgc
     3661 atccagatac agttccacgg ggtagccagc gtgtttcgcc gactcgtggg cgtagaggct
     3721 ggcggcgtaa ttccccgcga ccttgacatc gcccatgccc tgcggcgcgg cgcggtcgta
     3781 gtcatcgagg atcagcgcgc gcaccggttt cagccccccc ttgtaatagg ggccgaccgg
     3841 catgaccatc accagaaacg tgtactcgtc cgccggggcc acgccgatct gcgcgcccga
     3901 gccgatcagc agaggccgga tgtagagcga gccgcccgtg ccgaaaggag gcacgtaatc
     3961 ctcgttggcc ttcaccacgc gccggaccgc ctcggtgaac atcgccaccg gcacctgcgc
     4021 catacatgtg cgccgggcgg tgttggccat ccgctccgca ttggcctcgg gccggaagat
     4081 gcgcacgcgg ccgtcctgac aatggaaggc cttcaaccct tcaaacgccg cctgaccgta
     4141 gtgcaggcag gaggcagcga tgtgcatctt cagccagggg tcgctgacca gttccccttc
     4201 atcccacgct ccctgccgcc agacatagcg gatgtggcaa catgtatcca tgtatttgaa
     4261 acccagattg ctccaatcga tgttctgcat gcttcgaaat ccttgttggg gacgcgaccg
     4321 ccctttgtga cggatgattt gagattgtca tctttttcgg cgcacgatgc aagcccctgt
     4381 ttttcgtctt tcgcaaagcg cgcccgtcgg gcgcgaattc cccttttgaa gtttcggcgg
     4441 atttcgtgta tgttatctcg ttctgttttt ttgagaggtc gattatgatt cgagtcaaag
     4501 tggtcggcgc gaccggatac ggcggcgtag gcatcacgga actggtcttg cagcatccga
     4561 aggcgaagct ggtttccctg atcgcgcggg aggctgtcgg gcaaccgctc tccgccgtgt
     4621 atccgcatct ggcgggcttc tgcgatctgc cggttctggc ggcagaggat ccgcgtgcgc
     4681 aggaacccgc cgacgtggtg tttttcgcta ccccggacag agtgggcatg tgcggtgctc
     4741 cggaagagct ggcccgcggt gccaaggtca tcgattacag cggggacttc cggttcactt
     4801 ccgcagaggc gtacgccgat tacgccacgc ggatcggcaa agatccggtg cacgccgccc
     4861 cggaactgct cggacgcacc gtttacggtc tggccgaact gcaccgcgac gcgatccgca
     4921 cgagcaccct tgtggggaac cccggctgtt tcgcatgcag ttgcatcctc ggcatggccc
     4981 cggcggtccg ggagcgtctg ctcatgccgg acaccatcat ctgcgactgc aaaaccgggg
     5041 tgtcgggcgc gggcaaaaaa gtgaacgcgg cccaccatta tcccgcgcgg tacgagcaga
     5101 tgaatgccta caaactcgcg gggcaccagc acgtgtgcga agtcgagcgt gaactgggcc
     5161 tgctcgcggg tcaggaggtc cgcatcacct tcaccgcgca ggtggtgccg gtctgccgcg
     5221 gcatcctctc cacgctctac gggaccctga cgcacgccat gccggaggcc gaactcgtgg
     5281 cgttgtaccg ggatttcttc cgcgagagcc cttttgtccg cgtgttcgac agccagaccg
     5341 caatcggcac actgaatgtg cgcggcacga acttctgcaa ccttgttgtc agcgtcgatg
     5401 cgcgtgtgaa ccgtctgcgt gtggtcgcac acatcgacaa tctcatgaaa gggcaggccg
     5461 gttccgccct acagaacatg aacctgatgt tcgggttacc tgaaaccaca ggactcgacc
     5521 gaccggggat ctatccatga gcaataaaaa gtccgcccca aaaaaggttg tggccgccgc
     5581 aggccggaaa acggcccctg ccgcgtcccg caaacacccc aaaccgggtc ttggccgcgg
     5641 tctgggcgct ctcatcagcc gcccttccgg caatgcggtc cagaccccga acgtcgcggc
     5701 gcgcatggac gcgccggagc ggaccgggta tgacacagcc gcgccagacc cgcgccggag
     5761 cgtgcttgac gtgccggtcg cggagatttc ccgctcgccg tggcagccgc ggcagacctt
     5821 tgacgccgag tcgctgagtg aactggcgga ctccatccgg gcgaacggcg tgatccagcc
     5881 gctcatctgc cgcaaaaccg gcgacgcccg gtacgaactg gttgcgggcg agcgccgcct
     5941 gcgcgccgcc atcgaggcga aactcgcaac cgtacccgtg atcctcgtgg aggccgaaga
     6001 ccgccgggcc gccgagttgg ccatcatcga gaatatccag cgtcaggatc tgaacgccat
     6061 cgaggaggcc gagggttacc acacgctggc cgaaacgttc aacctgaccc agcaggaggt
     6121 ggcggaccgc gtcggcaagg cgcgcgccag tgtggccaat gccctccgcc tgctcgatct
     6181 gcccgacgag gtcaagcagc tcatcagcag cggactgctc tccaccggcc acgccaaggt
     6241 gctgctcagc ctgacggacg tgaccgagca gacgctcctc gggcgcaagg cggtgaccga
     6301 agggctgacc gtgcgcgcgc tggagaaggt ggttcagcgc cgcaacaaaa gggatgcggg
     6361 cgcgacactc cgccgccccg gccagccgga tctgcccgag agttatgtgc gcgacctcac
     6421 cgaccgcctc cacaaacact tcggcaccgc cattcgtctc tcttccagcg tgacgcacgc
     6481 gaacggcaag cacaccaagg gcattttgga gatcgatttt tacgacaacg atgatctgga
     6541 ccggctgctg accctgttcg gcatcacgct ggattgaacc gcgaggacga cgcgagggaa
     6601 ccggaatgcg gaggatgatg ctgggatggg ttttgctggg gctcacgggg tgcgacctcg
     6661 ggacaaccaa cccggagccc gtatccgcac cgccgccgca ggtgtggacg gccgcgcggg
     6721 ccagcaatgc cttcgcgtat gtcgaagggc tggtgcggga ctgcaccccg cgcgatgcag
     6781 gcacgccggg cggggagaaa gcagcctgct ggatccgcga ccgcctggcc gaacaaggcc
     6841 ttccagcgcg gatcgaccgt ttcaccgagc ccaccccgcg cggaccgcgc gaattccgta
     6901 acgtgctggc caccctgccc ggcaccgggg acacatggat tatcctgctc tcgcacttcg
     6961 acaccatgag cggcatcggg gaagggtttc agggtgccaa tgacagcggc tcctccaccg
     7021 ggttgctgct ggaactggca ggcgcgttgc gcgacgccgg accgcgcgaa ccctccatcc
     7081 tgttcgggtt catggacggc gaggagtgta tgatcgcgta tggcgcgcag gacggctttc
     7141 acggcagcaa acggcttgcg cggcagatga aagaggaaaa acggaagatc cgggccgtga
     7201 tcctcaccga catggtcggc gaccgcgatc ttaaactcac cctgccgcgc aacagcaccg
     7261 cagcgctccg tgtgctggct ttggaggccg cggaagccgt cggcctccgc gatcatatcg
     7321 gcctgtttga cggcaacatt tacgatgacc accaggcgtt tctggatttg ggctatccgg
     7381 cagtgaactt gatcgacttc tcctacggaa gccgtccggg actcaacgat tattggcata
     7441 cgcggcagga cacgctggac aaactttcgg tcgagagtct gcggatcacg ggcgcgatcg
     7501 tgctggaaat ggtcaaccgg ctctcgtctc ccgcccgccg ctgaagcgtc cgccggacag
     7561 cgcgatcagg ctcgcggatg ggccttggcg tacgcatcct tcagccggtc aatggaaacg
     7621 tgcgtgtaga tctgcgtggt ggagagactc gcgtggccga gcatctcctg cacactgcgc
     7681 atgtcggcgc cggcgttgag caggtgggtg gcgaaactgt ggcgcaactt gtgcggcgtg
     7741 agttcctgcg gcaggcccgc agcccggagc cacaccttca tctggcgctc gatcgaacgc
     7801 gaagtcaacg cgcccccctc gcggttgagg aagagcggcg cctgcggtcc cgctgtcgcg
     7861 ggccagaggc gcgtggccgc gtcgcgcagc cggatcagcg catcacaggc gggccgcccc
     7921 aagacgcaca accgttgttt gcggcctttg ccctcgacaa tcgtggtgcc cgactcgaac
     7981 cggatgtcgc gccagaccag cgcggcaacc tcgccgatcc ggcaccccgt gctgtagagc
     8041 acctcgaata tcgcggtatc gcgtaacgcc gcatattcgg cccacagcga gggcgcccgc
     8101 cccgcgtccg accgtttccg cagcgcggca agcggagccg ccagaagagc ctcaacttcg
     8161 cgcagactca gaacaacggg caggcgtttc ccgagacgcg gcccgcgcag ccccgcgaac
     8221 ggctgcgcgg tggttcgccc ctcgcgctcc agataccggt agaaggtacg caacgaagag
     8281 agcttgcggc gggtcgtggc gggcgcgtgc cccttacggt ggaaggcgga gagaaaggcg
     8341 cgggcccgcg cacggtcggg ttcgtcccat gcgaacggcg gccccccgcg cttctccgtc
     8401 ccccacacaa atgcggcata ctgcccgata tcctgaaggt accccgcgac ggtatgcgcc
     8461 gaggcgttgc gttcggccgt cagataccgg acaaactgtc cgaccgcagg gtcacgcgcc
     8521 acatcgatcc ggttcggcat cggctccggc ggggtgtcgc cgaaccgcac ctccattgtt
     8581 gtatcggcat tggcgtcgaa ttgcccatcc atgacattct cccggcgaat ccgggcccct
     8641 cccggaaacc cgttccgcct acgcttcctt tgtcccggag gtttccgtct tgtcggattt
     8701 gctcttcaac cccagtttgg ccgcataacg ctcaaacccc ggaatctgcg ccttgtaacg
     8761 gaacaccatc cgccgcatgg cataccgctg ccgcggcgaa agcgaacgct tgtggttgaa
     8821 ctgttccgaa agactgacga aaaaggcctg gtcatcgaag gacatcttcc cccgctgcac
     8881 cggagcctgc cactctgtga cgtgacccag catctccagc agcaccggac tctcgtggtc
     8941 ggtcgacggc tcgctctcct ccgccgtcag cccgagcgag gccttcaccg tctcgaaatc
     9001 gcggatctgc gccgcgttct gcacaacgat gcggtccagc gcggccatct gcttttcgct
     9061 cagctttcgg ccgctttccg cctgatgccg cagcgattcc acgaatttca tctggcgctc
     9121 ggagagttcc acctccttca gcaccgcaaa acgccgcatc acctcttccc cgctcggcgc
     9181 ggcgttgtcg gtctccacga tttcgccgaa accgcacgac ttcaacaccg cctcgacctc
     9241 cgggagttgc tcgcgatagc gcacggcgat tttggcaagt gccgtaagct gtttttcgga
     9301 aaccggtctc gcggatttct caatctgttc gcgcaccgat ttcacaaacc gttcgtcgct
     9361 gtacactcgt ttgctgttcc cccgctgcac cgcaggcgcc cagaccgtga cccgctccag
     9421 caacgacagc attgtctcca ccttgccggc atccgcgggc ggttctttgg cctgttccat
     9481 ccactgctcg aaccgcttgt aaaaatcatc cagcatcgcg gtccagccga tcccgcccgc
     9541 ctcgatctca tcaaggcgtt cctccatgcg cgcggtgaac ccgacatcaa acagcgaacc
     9601 gagtttcccg acgagcaggt cgttgacctc cagacccagc ggcgtgggca tcaactgccg
     9661 cttttcacgt gtcgtatagt cccgcgagct caatgtctcc agaatggagg cgtacgtcga
     9721 gggccgtccc acgccgtttg cctccagcgc ccggatcagc gacgcctcgc tgtaccgggc
     9781 gggcggcttg gtttccttgc gctcgctcag ccattccagc ggcaccagcc cgtcgccttt
     9841 agccagcggc ggcaaccgtt ggacctcttc gacctcctct tcgggatcct cctcctcagg
     9901 ctttttgcgc cggatgtcca gcgccatcac cttcaggaat ccatcgaacc gcacctccga
     9961 ggccgacgcg gtaaagaggc aggcgcggcg cggcgcgacc ccggttgacg cgggcgggac
    10021 cggttcgatc tccacggttt tctgcgcgat cttcgccgcc gccatctggc tggcgacgaa
    10081 ccggcgccag atcaggtcgt acagcttgag agaggaggcg tccaagattc ctttgagact
    10141 ttcaggcgtc cgggtgacct ccgtgggccg gattgcctca tgcgcctcct gggcactcgc
    10201 acggctgcgg tacacattcg gcgacgccgg ataatactcc ttgccgtacg cggttatgat
    10261 gtgttcccgc gcagccgcct gcgcgtcccg ggagacgttg accgaatccg tacgcatgta
    10321 ggtgatcaga ccgacaggcc cgcccgcatc gagcgcgacg ccctcgtaca gtttctgcgc
    10381 caggctcatg gtgcggctcg gcgagaatcc gcacacggta gaggccgcct gctggagcgt
    10441 ggaggtgatg aacggcggca ggggcctgcg cgtcgtttcg cggacccgca cctcccgcac
    10501 ccgcagcgtg cagccctcga gatcgctcaa cagcgcgcgc gcctcggttt ccgaaccaat
    10561 gaccggcttc tcgccgtcca ctttggcaag tttgaccgtg aacggatcca acggcgcaag
    10621 ggcgcgccgg acacgtgcgc ccatcaccca gtactcttcc ggacggaaag cctgaatctg
    10681 acgctcgcgc tctgacaaca gccgcagggc cacggactgc acgcgcccgg cgctcagccc
    10741 gcgcttgagg cgacgccaga gcagggggct gaccatatac ccgacgatcc ggtccaacac
    10801 gcgccgcgcc tgctgcgcgt ccacgcggcg catatcgagg tgccccggct tctcgaacgc
    10861 ggcgcggacc gcacgcggcg tgatctcgtt atacatgacc cggtggaacg gcttcccatc
    10921 ggccacctcc gccagcgtct cgcgcaagtg ccaggcgatc gcctccccct cgcggtccgg
    10981 atcaggcgcg aggtaaatct cgtcgcaccc tttgaccgcc ttctttaact ccgcgatcac
    11041 cttggttttg cctttcgaca aaacatactt cggcacgaag ccctgctcga tatccacgcc
    11101 cagcgtacgc tccggcaaat cccggatatg ccccaccgaa gacttgacca catagccgga
    11161 acccagcatt ttcccaatcg ttttcgcctt cgcgggcgat tcgacaatca ccagtttctt
    11221 actcatgtgc acctttcctc acatcggtta cgtcccaacc cgtacggccc aaatccgccg
    11281 cacccaaaca tcccccccgg gccggtcaac ttgcccggga cacgcgggtc acgaagcctc
    11341 ccggcaacag ccggaccacc cgcttgatct gcaaccccac cagcatcgcg ttcacggtgc
    11401 ccgcatccaa tccgctgccc cgcaccaccg catccaccga aacctcctcc atcccgatct
    11461 gcgccacgac cttccgctcg tcctcggaga ggacgcacgc cggagggcgc gcctcttccg
    11521 tcccgctccg cactgctgcc ggcgtctcgc gcgtcttcgc gccggccagc agatcctcca
    11581 cctcctcgat cacctcgtcc gcggtcgtga ccagacgcgc accctcgcgc aacaggcggt
    11641 ggcaaccctg cgacaccggc gagtcgatcc gcccgggcac cgccatcacc gtgcggttct
    11701 gatccagcgc ctgccccacg gtgatcagcg taccgctgtt gagcggcgcc tcgatcacca
    11761 acaccccttt gcagagaccg ctgacgatcc ggttgcgcat cgggaaggtt tggcggtccg
    11821 gctgcctgcc gaacgggtat tcggagatca ccgcaccgcc ctgcgccaca atctcgcgcg
    11881 ccaacccggt attctctttg ggaaagagac ggtccaacgc cccgcccagc accgccactg
    11941 tccgcccctt ggccttcacc gcgcccgtat gggcctcggt gtcgatgccg agagccaatc
    12001 cgctcaccac cacatatcct gcggccgcca gacggtaccc gaagaaacgg gcggtttcgc
    12061 gcccgtatac cgtgggccgg cgcgtcccga tcaccgccac ggcaggctga ttcaacgcct
    12121 ccgccgcccc cgcgacatac aggaccagcg gcgggtcggc gatctgcttc agcagggccg
    12181 ggtattccgg atccgcccat gtgaccagcc gaaccccctt tttctccgcc cgcgccagtt
    12241 ccggccccgc cgccacccgc cgcaattccg cgtgaaactg ccgcgcccgc tcggcgccga
    12301 tccccggcac actcaacaat tcggactcgg acgccccgaa cacggctgac aagtcaccga
    12361 accgttccgc catacgccgg gtcgtcaccg cacccaatcc cgaaatctga ttcaaggcga
    12421 tcaacgcctc acgctctgtc acgtccgcct ctgttcattg cttatcagga tccgccactg
    12481 ttgcaagaaa gtgcggtaat agcatattcc accctccctg acaagacagg ttgcgtttgg
    12541 acttttcccc gaccggttcc gaaacggcaa ttgacaaaaa agaaaacagc tttattttat
    12601 gggtatccac gcaaaaagca ggaatctgta cgaccatttc aagatcaata tcgtcggcag
    12661 taagccgaac gggacctgct ggaggtgatc ggcacggcac ccgtgccacg catctaaaaa
    12721 aggagactgg ataaatgagg agttgtctta aagcacggaa gccggtggcg tggtttgcaa
    12781 tggccgcctt gtcggtgtat ggcgggccga cgtcgattcc gatacgccag ggcggcgtgg
    12841 aagggcggcc gttctggaac aaggtgtcgc cgtggttcga gtatgcgccg gcgttcgatt
    12901 tccccgaggt caacggggcg gtgaagtacc gtttcatcgc gaaacaccag agcggtgcgg
    12961 aattttcgtt cgaggcggag agcccgcagg cggcgttgtc gccgatttgg gagaagttgc
    13021 ccgttggcgc aatcgaactg cgggtggcgg ctcttgacgc aaacggcggt ttcatcgaaa
    13081 ccgccccggg gaatgcgacc gttacaggca gtgcggttgc gatgaaaccg ccgacagccg
    13141 tgcgcaagtt ctggaaaaag gcgacgttcc acgaggggat gtacgagtcg gctgcgtgtt
    13201 cgtatgcaca gtcggcccgc aaggccttcg agttcgtgtt gaatgagccg tcgatccgct
    13261 atctccatga ccacggaacg cccgatccgt cctattcgct caacggctat ccatcgaaga
    13321 tgctcagtgc ggagatcggc attctcctcg atattgcaaa gacgcgtccg gaacaccgcg
    13381 cgttcgcgct cgaaaccgcg aagaaggcgg cagaccacct gatccgcaac cgctgcgccg
    13441 ctggcacgcc gcttgaatac ttcacgaaga cctatgaagg cgaaggggag tacggccggt
    13501 cgaagcagaa tgcgggactc aacatgctca tctatcccgc caaagccggc gaggcgcttg
    13561 tgaagttgca tgtggcgttg gcaagggacg aagggtatct ggatgcggcg gagaagatcg
    13621 cggcgacgta cttgaaactt cagggaacgg acggcacgtg gccgctgaag gtgcgcgagg
    13681 ataccggcga gccggtggcg ccgaaccgtc ttatcccgtt gccggtgatc gacttccttg
    13741 aatcgctcta tgccgttacg aaaaagcccg aataccgtgc agcggcgaac cgtgcgttcg
    13801 cgtatgtcga gaagggtccc ttgaagacct ggaattggga ggggcagttc gaggacacga
    13861 aaccgcgtcc gccttatctc aatcccacga tgcacgaggc ggcttcgacg gcgatctatc
    13921 tgctgaagcg ctttcccggg gacgcgaagc ggctcgaact ggcgatggaa ctggtccgct
    13981 gggccgaaga ccagtttgtc gcgtgggaga aacccgaggt cggcgacaga agagcgcatc
    14041 acttcaatcc cgcgaactgg tacgtgccgg gcacgatcga gcagctggca atgggcgagc
    14101 cgatcgacgc ttcgtccgca aagatgatcg aagtctatct cgcgctccac aaggcgaccg
    14161 gcgggcacga atactgggcc aaggcgcggg tgctcggcga cacgatcacg cgtgcgcagt
    14221 gggaagacgg atgctacaag accttctggg tgaaggcgga ccgcgacgac cagcggtacc
    14281 atgtctggat gaactgccat atctacacgg cgagggtgat gaacctgctg gcgaccgtgg
    14341 aacattggct gaacggtaag cctaaaagga gcagttgaac gggtagaatt ctagcaaagc
    14401 cccgtaaaca tcgggttttg acgtattgag atgcgggcgg agcccgcaat ctcaaaggct
    14461 gtaagactgt gaggcggtaa gactgtgagg cggttgtgtc ccgtggtgaa gcaatcgatc
    14521 ccgatagcga tagcgatccc gataccgata gcgatcccga taccgatttg gatgaaaccc
    14581 tgaaaccacg aaaccctgaa accacgaaac cctgaaacca cgaaaccaaa gagagttgaa
    14641 ggttcaactg ctctttttag ggtaagtgaa aagggtcggt attgacaaga ccgcccgggg
    14701 tttgtgataa ttctcaccgt aaaccttgtt ttgaactcaa tgccgcacac gcggcgtatc
    14761 tagggggtcg tttatgtccg atcatatgcc gatggccacg cgcgtcaaac tcagcgccat
    14821 gatgttcctg cagttcatga tgttgccggt ctggttcatc ccaatgttcc catatatcac
    14881 cgaaatggca cccaaaggca gtgccctgcc gttgctctgc ggcatgatca tgggcttcgg
    14941 tgccctggcc tcgccgatct tcggcatgtt cgcagaccga ctgatgaaca gcgagaaggt
    15001 gctggcgctc tgtaacttcg tgacggctgt gctgctggcc tacgcctaca cgatcaagac
    15061 tccggggatg ttgttcgtga cgttgctgct ggtgatggtg ttttacatgc cgacctggtc
    15121 gctgaccgcc acgatcgcaa tggcaaacag cacaaccgaa gccttcccgc agatccgcgt
    15181 gttcggtacg ctgggctggg tcgcggcagc gattttcagc atcatcggca ccaaggtttt
    15241 cggcatcgcc cagtttgaca gcaccccgta catctttttg ggcggggcga tcgctgcggc
    15301 actaggcggc gtgctggcct ttttcctgcc gccgacccaa ccgccggcca aggggcagaa
    15361 gatgtcgctg gtcgatacgc tggggctgcg cgcgctggta ttgctcaagc gccccgactt
    15421 tttggtgttc tccgtgctca tccttctggc gatggtgccg ttccaatggt acaacgtcta
    15481 caacagcacc tatctgaagg agcgcgggta cgaatatctg acgggggtca tgaaccttgg
    15541 acaggtgctg gaactggtct tcatgctcct gatcccggtt attctgaaga aggcgggcta
    15601 taaatgggct atggttttgg gattgctggc gctggtcttc cgttacggca tgttctatct
    15661 gggcgcggcg cacggactgc agatctgtga ctatgccggc attctgatcc acgggctgat
    15721 cttcggcatg ctcgtcgtcg gcagccagat gtatgtggat gcggcggcac ctgccgagtt
    15781 gcgcggtcaa gcgcagggct tcatcgggct gatcatgttc agcttgggaa ccttcctttc
    15841 aaacttcgtg tttgacggga ttctcaagaa gaacgtggcc gcagacggca cccatgtgtg
    15901 gacgaccccg tatctgatcg caatggtctc cgcgctggtg ttggcggtgc tgatggccgt
    15961 ggccttcaag cctgccgttc aggagaagcg gcagggcgcg tgagccccct gaccgcctag
    16021 gcgggaaccc tgcggaacgc gccgtcacac gcctgtcgaa ccgaagccgg ccgttccgcg
    16081 cacggtcccg ccgagttccg cgacttcccg cacctcggcg cgcgccaccg gcgcgatcac
    16141 catctgcgcg atccgcatcc ctgcggtaat ttcaaacggc gtgtcgccgt ggttgatcag
    16201 gatcacgccg atctccccgc ggtacccctc gtccaccgtg ccgggcgtgt tcagcagcgt
    16261 caccccgtgc cggagcgcca gaccgctgcg cgggcgcatc tgtgcctcgg ttcccggcgg
    16321 cagcgccacc acaagaccgg tgcggatccg tttgcgctcc ccgggttgaa gcacggccga
    16381 ctcgcaggca ctgacatcca tgcccgcatc accgggatgc gcatacgacg gcaccacggc
    16441 ttcgggatgg attctcatga attccactgt catgcccaca gcataacaga gccgacgccg
    16501 gtatgacaac ccgtggtcgt cagtgctgtg ctgaaacgtc ccgtgaacgg ggcgcacaga
    16561 accgccctcg gccctgtgcg cggtgtcggt tctgtgcgcg gtgtttacac cgccagccag
    16621 ccagcggcca gcgttgcgct ggccgccata tgtcttgtgc ccttatcccg ccgtgatcgc
    16681 gatccgtcgc ggtttgacct cctcgcgttt gggcagcgtc agcaccagca tgccgttttt
    16741 gcaggatgcc tgcaccttcg ccgtgtccac gcgctcggga agctcaaacg ccgcgcgata
    16801 ccgcgcctcc tgaatctcat tcaggatcaa ggtgtagtcc ttgaaggaag ccacctcttt
    16861 ttcggccgtg accgtcagcg tacggtcctc caaagtcaac tcaacctgtt tttccgacac
    16921 gccgggcaac tccatcgtga gcgtgaacgc ctcttcgctt tcggtgatcc ggctcacagg
    16981 ggtcagctcc aaacggttgg ccttctcggc ttcaactcta tcacgtttca tcaagctcat
    17041 gctctcctcc tttcctttcg cgacccggtc acagtttctc aatggcgatc ttgcgcttct
    17101 ccgccgccgc cttccgggga atggtgacgg tcagcacacc gtttttcacg gtcgccttga
    17161 tgcctttctc atccagttcg aagggcaggt tgaaactgcg ctggaactca ttctccgtgc
    17221 cgtccggcaa ctcgcgcttg cctttaagcg tcagcacgtt cgcgtcaacc gccacgtcca
    17281 gcgtatccgg ctccatgccg ggaacctccg cttcgaccac cagacctttt tcgctctccc
    17341 acacgttcac acgcgggttg gcgccaacac gggcacgcca agccgaattg aagaggtcat
    17401 aagggaaatc acccatcaac tcgtcaaaga cacgccaggg attgccccaa ggactcagat
    17461 atgctctcat ggtatttctc ctttccttta acactctatt tttcggaacc aacggaaccg
    17521 cctatctctt cgcattgtcc atgccaactt tttcataccg atctttgtaa tacgtaagac
    17581 attatcacac tatgagttat ataaaaaaac aatgccgtga cacacggccc tgtttgggtc
    17641 aacatgactc tttttgccga ccgcatgaca ttatgtcaca gttttaaggc ccaaaaatgc
    17701 gggccgcttc ttccgggcct tttgaccaaa gtcttttcta ccacattttg tggttgaatt
    17761 cgttgccgga tcaatatata gtgcggcctg ttattcgttt gattctttta agtcctcttt
    17821 ttatagagga atccgcacgt ccgatagaga cgggtgcagg ggggaagggt ttttatgagc
    17881 aggtatgagc agccagactt gattctccgg attcgtaaac gtgacggtcg ttcggaggtc
    17941 ttcgattcga ataaaattgt gggcgccatt cttcgggccg gagaagcgac aggcgagttc
    18001 gccggggacg aggcgcgcaa actgaccctc aaggtcatgt cgatcatcca ctccctcacc
    18061 gacacgcttg acctctctgt cgaaaacgtg caggacatcg ttgaagaggt gttgcttgcc
    18121 tcgccctacc gccgcacggc caaggcctac atcatttacc gcgaccagca cgcccggatc
    18181 cgcgaaatgc tccgcaaagc ggacgtggac ctgatcgacc ggtatctggg caaagcggac
    18241 tggcgtgtgc gcgagaactc caacatggcc tactcgctgc aggggctcaa caactacatc
    18301 tcaagcgagg tcagcaaagc ctactggctc aacaaaatct atccggcgga gatccgtgag
    18361 gcgcacgaca acggcgcgtt gcatctccac gatctgaatc tgctgagcgt ctactgcgtg
    18421 ggatgggatt tgcaggattt gctgcgcagc ggcttcaagg gcgtggccgg caaagccgaa
    18481 agcctgcccg ccaaacattt ccgtaccgcg ttggggcaga tcgtcaactt cttctacacg
    18541 ctgcagggcg aggccgccgg cgcccaggcc ttctccagct tcgataccct gctcgccccc
    18601 ttcgtgcggt atgaccggct caacgccgcc gaggtccggc aagccttgca ggagttcatc
    18661 ttcaatgtca acgtgccgac gcgcgtcggc ttccagacgc ccttcaccaa cttgacgatg
    18721 gacctgatcg cgccgtcgac gctggcgaac gagcatgcgg tcatcggcgg caaggagatg
    18781 ccggagacct atgccgattt ccagcacgag atggacatga tcaacaccgc gtttctggag
    18841 gtcatgaccg agggcgatgc caaggggcgc gtcttcacct tcccgatccc gacctacaac
    18901 atcacccagg atttcgactg ggagcgccct ggtctggaga agctctggga ggtcacggcc
    18961 aagtacggca tcccgtactt cgcgaacttc gtcaattccg acatgagtcc ggacgacgcc
    19021 cgctcgatgt gttgccgtct gcgcctcgac acgcgcgagt tgaaccggcg tggcggcggc
    19081 ctgttcggtg ccaatccgca gaccgggtca atcggcgtgg ttacgatcaa tatgccgcgg
    19141 ctcggttacc tcgccaccga tgaggacgat ttcctcgccc gcctggacaa ccttatgggg
    19201 atcgcgcgca acagtctgga aatcaagcgc aaggtgctgg aacagttcac cgcgcagaac
    19261 ctctacccgt acacgcgttt ctatctgcgt caggtttacc agcgtttcgg cacctattgg
    19321 cagaaccact tctccaccat cggactcgtg ggcctgaacg aggcgtgcct caacctcttc
    19381 ggcgaggata tcggtagcgg gcgcggtcgc gcctttgcct tgaccgtgat ggatttcatg
    19441 cgcgcgcggc tggccgacta tcaggaagag accggcaacc tctacaatct ggaggcgacc
    19501 ccggccgagg gcacgaccta ccgtctcgcc cggctcgacc ggaaactcta ccccgagatc
    19561 atcacggccg gcggcgaggg agagcccttc tactccaact cgacccagct tccggtcaat
    19621 tacagcgacg acatcctgcg tgtgctggat ctgcaggatg agattcagac caagtacacc
    19681 ggcggcacgg tcgtgcacgc cttcatcggc gaggcggtca gcgacccgca ggcagtcaag
    19741 gcgttcgtgc gcatggtctg ccggaactac cggttgccgt acttcacgtt gacgccgacc
    19801 ttcagcatct gcgaggatca cggctacctg tccggcgagg tgcatgagtg cccgcactgc
    19861 ggacagtaca cggaagtcta ttcgcgtgtg gtggggtaca tccggcctat tcagcagtgg
    19921 aacgcgggca agcagaacga gttccaccag cgcaaaacct accacgtctg cgaggcggcg
    19981 gcgcgatgaa catcgcgggg gttgtgccgc tcagcctctg cgattatccg ggccgtgtgg
    20041 ccgccgtggt gttcacgcag ggctgtaatt tccgttgccc cttctgccat aatgcgcacc
    20101 tgatccccct cctgcccgta gccggacaga tgctggagga agaacaggtg ttcgcccgga
    20161 tcacggagta ccgaacccgt ctggacggag tcgtggtctc cggaggcgag ccgaccctcc
    20221 agcaggatct gccggagttt ttgctgcgcc tcaaaacgct cggcctcgcg gtcaaactgg
    20281 acaccaacgg ttcgcagccg gaaatgctcc gccgcgtgct cgacgagcgt ctcgtggatt
    20341 tcatcgccat ggatgtcaag gccccgtggg aacactaccc gcgactcacg ggcaccgcgt
    20401 gtgctcagga tgacctcttg cgaagcctgc ggctgatcgc cggttccggg cttccccacc
    20461 agttccgcac cacgcgcgtg gacccgttgc tgacggacag cgattaccgt aatgtgcgcg
    20521 cgcaaattcc ggacggcagc ccgcatgtct ggcaagcctt ccggcccgaa cacgcgtttg
    20581 atcccgcgct gcgccctccc cgtccgtgca ccgataacgt gcccgtaagc gtgtcgtccc
    20641 gtctttctga ttgcccgcag gcaagggaga gggcacaata gaggcatgtt cgttccgaac
    20701 gccgataaca tcacggcacg ctgggtctgc cagatcgcgg gcggggggat tctggtcgcg
    20761 ggagttgccg cagcgctcgc gctgttgtgg tccgcgcaac gtcgtcgcgc cgcaggcgcg
    20821 cggaccgtcg ccgtcgcgca cctggcctcg ctccccctct cggatgttca cgccggagtg
    20881 accctgatca tcacgttcct gttcgctctg gcgtccttgc ctcggacggc agggaccgcg
    20941 tttcggcctg acgaccgcgc gctggtgctc ggtcccctgc tttatctctg ttcgagtgtg
    21001 ctggtcgtga tgttctgtgg cgtccacgcg aggtgcggcc tgcggaggat gtttctcgcc
    21061 ggaccggtta ggccggccgc cgcagtggcg aaagggctgg tctacggact cgccgcgatc
    21121 ccgccggtgg ccctgctctc gttcctgatt tccgcaggtc tggaggcttg gggtttcgca
    21181 ccgcaacatc aggaggtctt tgactggctt ggcgatgcct cgcgggcgcc cggggcacgc
    21241 ctcttcatga tgggggcggc cgtgtttctc gccccccttt ccgaggagac cctgttccgg
    21301 ggcatcctct tcccatgcct gctgcgcggg aggtcggttg tgggcgccgc attggtttcc
    21361 tccctctatt tcgcgacggt gcatcttcac ctcgcgtcct ttctgccgct ggtggtgctg
    21421 ggcatgtttt tttctgcggg ttacgctgcc accggctcgc tcctgacccc catcaccatg
    21481 cacgcgctct tcaatttcac cagcctgatc ttttatcttg ccgatcccct tccctgaatc
    21541 cggcgcgcaa gccggccacc cccacaagca gaacacgcac atgacggatc aagatctttt
    21601 cgacaaatcg gcgtcatcgc cggagttccc gacgggacac gccgcagagg gcctccgccc
    21661 cccgttggcc gcacgcatgc ggcccgaaac gcttgaagac gtggtggggc aggagcatat
    21721 tctcggcccg ggaaaactgt tgcgccgcgt gattgaggcg gaccgcctga ccaacctgat
    21781 tttctacggg ccgcccggat gcggcaaaac caccctggcc gaagtcatct cccgcatcac
    21841 ccgcagacgg tttgagcgga ccagcggggt tctgtctaat gtcgccacgt tacgctctct
    21901 ctgtgagacc gcccgcctct accggaacga ccccggcacc atcctgttca tcgacgagat
    21961 ccaccggttc agcaaatcgc agcaggatgt gctgctgccg tatgttgaga acggcacggt
    22021 aaccctcatc ggtgccacca cccacaaccc gaatatcttc gtcaacagtc cgctcacctc
    22081 acgctccctg gtgttcgaat tgcgaccgct gagcgaggaa gccatcacct gtctggtgca
    22141 gcgcgcgctg gccgactcct cgcgcggatt gggtcatctt ccggccgaac tcacgcccga
    22201 ggccacacag tttctcgcca ccatgtgcga tggagacgcg cggcgcgcgc tgaatgcact
    22261 tgagatcgcg gtccgctcca cgccgcccga tccggacggg gtgatccgca tcacccacgc
    22321 cgtgatcgag gactcggtac agcgcaagat ggtcgtctat gataaagatg aggacggcca
    22381 ctacgacacc atttcggcct tcatcaaatc ggtgcgcggt tccgatcccc acgccgcgat
    22441 ctactggctc gccaaaatgc tggaggccgg cgaagacccg cggttcatcg cgcgccgtct
    22501 gatcatcctc gcctccgaag acatcggcaa tgccgacccg cgcggactca cggtggcggt
    22561 cgcggccatg catgccgtag agttcatcgg tatgccggag gcccgtatct ccctcgcgca
    22621 ggccaccacc tatctggcga ccgcgcccaa gagcaatgcg tcgtacctcg ccgtggaaga
    22681 ggctctggcg gacgtgcgct ccggccgtgt cttggcggtg ccggagcacc tcaagaacgt
    22741 gcatgtccgt gccgtggggc gggagggact cgacacggct tacaagtatc cgcacgatta
    22801 tgcccagcat accgtggatc aactgtatct gccgaaggac aagcgctatt accgtcctgc
    22861 cgcgcaggga tatgaggaaa ccctcgcccg gcgcatggaa cacgtcgaac ggctgaaacg
    22921 cgaaacacgc gaatgacgca tgcccgcgcg accggccagc gcaacacctg aagttttgaa
    22981 agcggccacg cgctttgcac aggcattgat ctgcacagac cggctgacag gcagatatcc
    23041 ggggtgattg tcgtaccggt catttctttc tatcaggacc acgtatcagg aaaatcgatc
    23101 ccgatagcga tcccgatagc gatcccgata ccgata
//
LOCUS       DAOSLE010000025        22625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2534_length_22625_cov_9.200665, whole genome shotgun sequence.
ACCESSION   DAOSLE010000025 DAOSLE010000000
VERSION     DAOSLE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 22625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22625
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2534_length_22625_cov_9.200665"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1638)
                     /locus_tag="PK576_03250"
     CDS             complement(304..1638)
                     /locus_tag="PK576_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE60637.1"
                     /translation="MTLYETDSLFPWNRLEDSPDLQTIRDFFRQLPDGPLLAALAARR
                     GRGRDDYPVGTMWFCALLQPLLRHPTMQLTLDELRRNADLRRLGGMESVAEVPKPWNM
                     SRFLALLGREPCRAMLAGVFDAMVRRLGEAVPDLGRHTSGDSTHLSARRGRSRAGRGE
                     MQPDGGRKEYTDKDGRCVKVLDWFGYKLHLLCDTRHEVALSYRVTPASAHDSKSIPEL
                     VEKARGNLPEGRMETLAFDMAADDTGTHRLLAGRGIKPLIETRSLWKDEAERVLEDAG
                     IGNIVYDEAGTVSCYDMASDPPVRHEMAYIGHEAKRGTLKYRCPAMHRKWDCPCHGRC
                     NRDKRYGLTVRVKQSIDLRRFPPIPRATRTFERLYKGRTASERVQARLKIFWGVDDGN
                     VAGGAGFLASVGLVMVAHIGLAALLAGSPRRRAGTLGRVRLGSVAKALQGNR"
     gene            complement(1971..2399)
                     /locus_tag="PK576_03255"
     CDS             complement(1971..2399)
                     /locus_tag="PK576_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60638.1"
                     /translation="MNNTQRIWVWGTVLMAGMLATVPARADRVSVGVGLSTGNGFLTL
                     GINQGGHGHFHRPRAFHPRHPLPRPRPIGVLPPPVVVVPPPVVVAPAPVMVRSGYWQE
                     REERYWVEGCWIETLDTFGRRCKQWQPGHWEVRRIREWVY"
     gene            2576..3292
                     /gene="pyrF"
                     /locus_tag="PK576_03260"
     CDS             2576..3292
                     /gene="pyrF"
                     /locus_tag="PK576_03260"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOE60639.1"
                     /translation="MAELIVALDVASTAEVARTVDLLGDAVSFYKIGLELFSAEGPEV
                     VRAVRKRGKRVFLDLKLHDIPRTVERAVRAGAALGVELMTLHAAGGRAMIRAAKEGAR
                     SAGADAPRLLAVTMLTSLDQADLADFGVARSMAEQVEALGRLACESGADGIVCSPQEV
                     ARMRTVLGADALVVTPGVRPAGAEIGDQKRVATPAAAVRNGSTHLVVGRPILEASDPC
                     AAARAIVAEMSAAAYECGGV"
     gene            complement(3343..3978)
                     /locus_tag="PK576_03265"
     CDS             complement(3343..3978)
                     /locus_tag="PK576_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60640.1"
                     /translation="MPVKIPPSGQVATPSAGSAAQPARARPRPRPPTVRRNPSRAARK
                     DVAAPSAAGTAAVAEAAAQSTVPAVSSSTVARSAPREQAVKKWEAFVDQITERETPPE
                     AGQMEQFKNEFMRLHADDQVDAIQNALNLLPDEQFVSLFAILFDKTQNPDVLDEIFND
                     ALNRPEEIKVPMMKALLRDKGHPLHDEVARILDATGEGDADDGNGAEAATP"
     gene            complement(4175..4747)
                     /locus_tag="PK576_03270"
     CDS             complement(4175..4747)
                     /locus_tag="PK576_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE60641.1"
                     /translation="MTLDRTYHPTLWRTCRVLANARRLACLRVVMGTPNLCVQEIARE
                     VRFDEAQVSLALRALQARGLIAAVRISRWVRYVPEPDPSVPSAKPLLTALRRALRSGG
                     MSDAEIIRVVTAFTHPRRLAMLRCLHLRAPLSLETLSTACGISMPAANRHFHKLLARG
                     MLVVRSDRISLVSRHPSLTRALLRLAVCGE"
     gene            complement(4744..4950)
                     /locus_tag="PK576_03275"
     CDS             complement(4744..4950)
                     /locus_tag="PK576_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60642.1"
                     /translation="MGTPNLCVQEIAREVRFDEAQVSLALRALQARGLIAALPFGRQR
                     VSDEITLDLRQCQPYPFHTLKGVK"
     gene            complement(5583..6545)
                     /locus_tag="PK576_03280"
     CDS             complement(5583..6545)
                     /locus_tag="PK576_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE60643.1"
                     /translation="MNIPPIIRRLLGLVEAAAFLGLLFTLAGLFGRFGWLFEIFTHFK
                     PQFALCFLGYAAFECLARRYRRAAAGLLLAGVNALPVLLLLLPAAPVSLPRSPQPAVR
                     LRLLQANILTHNPDASALLRLVEREQPDVVILQEPNTRWLRDLASLTNRYPVYASMPR
                     EDHFGAAIYCRTNALEAGIFQLDDPEGAPSTCARIRIGDRTLTVIGTHALAPYNAAMW
                     AGRNRFMRMLAQHLRSLGTPLVVTGDFNNTPWSAHFRTFLRESGLIDSAQGRGPLPTW
                     PASLPFLRIPLDHGLHSSDVRIINRRRGPDIGSDHLPLIFDLAF"
     gene            complement(6542..8641)
                     /locus_tag="PK576_03285"
     CDS             complement(6542..8641)
                     /locus_tag="PK576_03285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOE60644.1"
                     /translation="MSAFLHIPYLPPFDRLTPEAAASDFPHLLADAHAAVTKVEQALT
                     PSWEGLMRPLYDACRPLFDAWGLLSHMLSVMNSDAWRQVQERLQPEIIAFSQRVAQSP
                     LLYRGYLALREADRASPCLTPVRRRILVKTIQAVEHAGVGLDAERQTRFNAIQNELAQ
                     LATAFRNHVLDATRAFALTLRTPEEAAGLPPALREVTAQAARAAGEPDATAATGPWRI
                     TLDTAVYLPFMMHSRNRTAREQLCRAYATRAASGPTDNTLKIERLLALRRELAGLLGF
                     ESYAELNLADKTARSVAAVDTLIARLAEAAGEAARTESEELLTFARSHGFDEGALQPW
                     DIAFWSERQREHLYDYSDEELSRYFLFPRVLEGLFSLANRLFGIVITPADGEAPVWHP
                     DVRFFRVADADGTPLACFFLDPYSRPETKSGGAWMNDFRTRDRAPDGTVTLPMALLVC
                     NQSVPVGNQPPVMRFQEVGTLFHEFGHALQHMLTTVEEPGASGLNGIEWDAIEIVSQF
                     MENWCYDRATLKGLSRHAETGDPLPDALFDRLIAAKNHRAASAMMRQLFFAATDMDLY
                     ARYPRPEWPDADTVKRLNAARYSPTPLLPEDRFLCSFSHIFAGGYAAGYYSYKWSEVF
                     SADAFAAFEEAGLAQGDAVRAMGRRFRDSLLALGGGTDPAEVFRLFRGRDPSIEPLLR
                     HNGLLRAELRNPAPPAS"
     gene            complement(8683..10191)
                     /locus_tag="PK576_03290"
     CDS             complement(8683..10191)
                     /locus_tag="PK576_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60645.1"
                     /translation="MKPIELIALRPLIGLGCAVLGAALLPLLAGCLISGAPHASQTDP
                     STSEEADARRAGALALMAQGLLVESGEGAETNRAKAQAAALTAFRQAVRLDPDGREPL
                     DVLLSALAEREQFAEGFAAAENFLTRHPDDTAIRIEAVRLAEAAARPLEAARHCSLLM
                     EIEPDNRELALARIRLYFMADCENEALEAMRACHARFHDRGSAVLPAHWAVHFLREQE
                     SNPARALRCVDLALAQADEETPVWRGGLHAVAAECHFSLAQTNDALASLHAACREDPG
                     ATGPVLRLGAIWAADPDATNRLAARALREPDTAPTRLILAAVHQEQNNRPAAIATLRE
                     HYTRRLCAGYFPDERFYLWLAGLTEAEKDAAGTERILREALTVHPTSDALQNFLAYLW
                     AEQDIHLDEADRLVTEALQAAPDNAAYLDTKGWILFKKSRPYDALQFLLKAADLDRSE
                     PVILDHTGDVLQAVGRSAEAAAFWTRSHEIDPTPAVTEKLRRHGVAPKPTTP"
     gene            complement(10308..10397)
                     /locus_tag="PK576_03295"
     CDS             complement(10308..10397)
                     /locus_tag="PK576_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HOE60646.1"
                     /translation="MGSIKKKRRAKMSKHKHRKRLKSNRHKNK"
     gene            complement(10452..11321)
                     /gene="hisG"
                     /locus_tag="PK576_03300"
     CDS             complement(10452..11321)
                     /gene="hisG"
                     /locus_tag="PK576_03300"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOE60647.1"
                     /translation="MAVILGLPKGSLQEATFKMMRKAGFVVQAGSRSYSPSVNDPGLQ
                     CRLIRPQEISRYVELGLLDAGITGYDWIYENNSDVHEVSEMCYSKATSQSVRWVLAVP
                     NDSPIQSVKDLQGKRIATEAVGLTRRYLAQHQVEAEVEFSWGATEVKAPELVDAIVEL
                     TETGSSLRANNLRIVDTILTSTTRLIANHKAWKNPAKREKIEQLAVLLQGALAAESRV
                     LIKLNVPADRLDTVTELLPSLHAPTVNRLNKRDWYAVESVVEEQVVRDIVPPLRNAGA
                     TGIIELPLNKVIF"
     gene            complement(11433..11517)
                     /locus_tag="PK576_03305"
     tRNA            complement(11433..11517)
                     /locus_tag="PK576_03305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11481..11483),aa:Leu,seq:tag)
     gene            complement(11578..13836)
                     /locus_tag="PK576_03310"
     CDS             complement(11578..13836)
                     /locus_tag="PK576_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE60648.1"
                     /translation="MRLNFTFIGIVALCAAFPAMLTARITDQWRYTLQEPEGAWQAPD
                     FDDTAWKKGSGGFGTAGTPGARVGTQWRTRTIWLRKRFTFARDVAEPALLVHHDEDAQ
                     IFLNGQPVLSLNGFLKEYKVFPLDAAQRALLRPGENLLAVHCRQTTGGQFIDAHVIDA
                     AAVPELPIPERELFSSALITPWGEKVTDQNAWAGYPRPQLRRKAWQNLNGSWEMAVTP
                     VARQEPPLQWQGRIRVPFCLESKLSGVQKMLRQDQALWYRRTFTVAAAERTLLNFEAV
                     DYRCRVFVNGRDVGGHQGGFTPFTLDITDAVRVGENELLVRVEDEQEGFQLRGKQTLM
                     PHGIWYTQVSGIWQTVWLEQVGRTYLADFALRTDAATGTITVTPKVTGTAETLRVEVL
                     ENAKRVAEVAGKAGAPISVRVEKAKCWSPASPHLYDIKLTLLDKDGKVLDQVESYAGI
                     RTVGKTRDAQGHLRFTLNGETIFHWGPLDQGWWPDGLLTPPSEEAMAFEIDWLKRAGF
                     NMIRKHIKVEPRLYYYHCDRIGMMVWQDQVSGGKGAKWTFLKPEPRDAEWPEAHHAQF
                     MLELGRMIDTLENHPCIVTWVPFNEAWGQHRTLDVGAWVARRDPTRLVNIASGGNFWP
                     VGDVVDAHSYPHPAFPFEMGKDGRFDSFIKVVGEFGGHGYLAKGHLWDDERENWGYGG
                     LPKNKAEYKERVAKSITLLNELRQKGIAGGVYTQTTDVEGEVNGLMTYDRKVMKIAAE
                     ELAGLTSVLFAP"
     gene            complement(13883..16366)
                     /gene="gyrB"
                     /locus_tag="PK576_03315"
     CDS             complement(13883..16366)
                     /gene="gyrB"
                     /locus_tag="PK576_03315"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006340295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE60649.1"
                     /translation="MSDDTRSSSQLDTAQAETHTEKYTAANITVLEGMKAVRKRPAMY
                     VGDTGERGYHHLVYEVVDNSIDEALAGYCTHIDVTINPDGSLSVVDNGRGIPVDLHPV
                     EKRPAVEVVLTTLHAGGKFDGSNYKVSGGLHGVGVSCVNALSEWMEVEVRRNNRIYRQ
                     RFERGHPVTKLEVVGETHGTGTKVTFYPDHTIFTCHEFKWDILANRLRELAFLNRGVS
                     IRFRDMEHEQERDESFLFNGGIVEYVQFLNRNKTPVHDEVIYFSGTRDRVECEIAMQY
                     TDSYNEIEYSYCNNINTIEGGTHLSGFRSALTRTVNKYIADNNLQKSNEEALTGDDIR
                     EGITVVVSVKVPQPQFEGQTKTKLGNGEVEGIVAQIVNDQLGTYFGEKPAVARKVVEK
                     AVLAARARIAARKARDLTRRKGVLDSLALPGKLADCSERDPAKCELYIVEGDSAGGSA
                     KQGRNRETQAILPLRGKVLNVEKARIDKILNNNEIRTLITAIGAGFGADEFDISKVRY
                     HKIIIMTDADVDGAHIRTLLLTFFFRQMPKLIENGYIYLAQPPLYKLTRKQREEYIES
                     DDQLTSKLLLLGADDMTVRTPEGRVLDAKELRRLLELLAQIENTLRAVARRGIDPDTF
                     LSLRKPETGEFPRFATILGSGEDAVFRFVYSDADLANARQEAESKLGAAIDLTETQVQ
                     AYHPTQPMPFRWLEMYRAHLLKKQIDSLVGAGFTVEQVAGGDAPAVFIEEEDESIPVR
                     ALPELLNTVRERGRKGLSIQRYKGLGEMKPEQLYDTTMDPAKRRLLRVRLEDAVKADA
                     IFTLLMGDEVEPRRKFIEDNALNVRNLDV"
     gene            complement(16564..17295)
                     /gene="ubiE"
                     /locus_tag="PK576_03320"
     CDS             complement(16564..17295)
                     /gene="ubiE"
                     /locus_tag="PK576_03320"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOE60650.1"
                     /translation="MKVEHITPYGAGEGKREQITRAFDAIAPGYDRLNRLLSLGLDRF
                     WRWRALRLLRAAPSARVLDVATGTADFAIAVARRLPQTRVTGIDLSEAMLDLGREKVR
                     RAGLGARVSLHRGDTLALDFPDASFDTVTAAFGVRNFASLADGLREMRRVLKPGGQVL
                     LLELSRPERFPLRLLHDVYLRVAVPVMGRCLGGHAREYAYLPASIRQVPQGGAMLDLL
                     REAGFRETRCERYTGGICSAYCGVR"
     gene            complement(17285..18766)
                     /locus_tag="PK576_03325"
     CDS             complement(17285..18766)
                     /locus_tag="PK576_03325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOE60651.1"
                     /translation="MSEHNHRDILLVAVNARYSHCSFAARSLLANLGTLERQAAILEC
                     DLEITPVQLAERITAHNPRIVSFSVYLWNVRLIESTARILRLVAPDLKLVAGGSEITS
                     EYPHVNLFDAVIIGEGEAAFRAFCETSIPSPAPSLSTSPSQRDPNSQLLTPNSQFLIP
                     NSLTLPYHLYTDEDLSRRTIYVETSRGCPYNCGYCASAGSGLRLLPLDRILPAFDRLW
                     QRGLRRFKFLDRNFNAPVAHACAVLDFFQERVTPKDTCLHLEINPERLHPDVADRLAR
                     FPAGTLHLEAGIQTLNPQVAAAAGRSPDTATTLANLRFLTRETGADVHADLIFGLPGE
                     DEASFANGFNTLVTTCAPPEVQVNLLKGLPGTRFAREADSLGLVFSPEPPYGLLRSDT
                     MDFETLCRIQRLARCWERVHNRGRFPHAVRAFHAACGPDLFGAYTRLAARLYAGEGRL
                     FAIGMPRLAGYLREELTETCGLGAAEADRLLTADLNAKTLHEG"
     gene            complement(18835..21045)
                     /locus_tag="PK576_03330"
     CDS             complement(18835..21045)
                     /locus_tag="PK576_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOE60652.1"
                     /translation="MDNRKRSSGTKQGYSFGTFGGVFTPSVLTIIGVIMFLRFNVVVG
                     YAGLWNALLILAASKAITVITALSLSSISTNMRVKGGGAYYLISRSLGPEFGGVIAVF
                     FYIAQTVAVTMYIIGFTEAVFSAFPNIPLSFRDVATLSNFIVFLVVYIGANWTIRVQY
                     WILALMMLSILSFFIGGALGFSPEILQSNLAPEWKDGHSLFTIFALFFPAVTGIMAGV
                     NMSGDLKDPSRSIPRGTFAAIGATGLIYALVTFLLAGSVPRAELISDGFVMKDRALFS
                     PLIYAGVFCATLSSALGSMMGAPRILQAFARDDVFRGIKWLGRGSGKNNEPRFAIALT
                     FVIAQAGIMVGDLDTVAPVITMFFLMTYGTVNLACFYEFISRNPSFRPTFRINHWIIA
                     LAGAVGCLVVMFLINAFWAFIAILLALLLYSVIRRIEIMVKWGDMQSGLAYQRARNAL
                     LRLEQERYHPKNWRPTILALSGGAYNRIHLAEYACWLSASHGIVSIGQVIRGNLDNLV
                     ARQREAEFLLRKFIREEGLNAFPAVVVDKNIHAGVQGMLQCHGLGGLRPNTLLLGWSQ
                     DAEKSEVFFGLLTLAKRMERSIVIVANKALDKEAHTVIADGAINIWWNNERDASLLLL
                     LAYLLKKNSEWRTRPIRILRPVPYKADVMSISAAMREMLEGARIEAEIVILPCANPTF
                     AVRTAMQPSAVLFASFDPPDEDNEAATAARMKKIVALPGDVLLVYNAGYNSLQA"
     gene            21409..>22625
                     /locus_tag="PK576_03335"
     CDS             21409..>22625
                     /locus_tag="PK576_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60653.1"
                     /translation="MKRSMLALACAGLSAVAASAGEAAVPMSSPDTNPYITFSSSLKF
                     YVWPQKSSDGTLEYSDNAKQWLTNTTANMEAVGDSGVYKLYVRGTGNTRITADSPPHG
                     WQITADNPDTRVACSGNLATLLDYKAVKNGQHPPMDEKCFAHLFEGCTLLSSAPSPPA
                     ANLTYACCRAMLEGCTNLTNAPALPATTLANACYDSMFKGCTALTRAPELPATNLALG
                     CYAYMFIGCGSLTKAPALPATTLAPACYRQMFGSCGSLTKAPALPAMTLAGSCYLYMF
                     YDCPGLTELPVLPATNLVANCYDKMFQRCSNIKVYTTGPGVQWSIPNNATTTFATNWN
                     YRMFDGTGGTFKDAPEIGTPYYYTLPVPFPDSDACLTFSSAGAFTVAPQEVSWDGSLY
                     ASTDTTNWMAFAT"
BASE COUNT         4434 a         6962 c         7085 g         4144 t
ORIGIN      
        1 actcctaaat cctaaataaa tcctacatcc gcgcgtagcg cggaagacgc ccgcgctcca
       61 ctcacatgcc ccctgtcacg agagccgatt gcgctgtgag cttggagcga gggtagcgcg
      121 gggcggggag cggatatctg tgggctggca agctcgggat tcgtgtttgc cgcgcccctg
      181 gaactgcgga ttgcgcggga aagcgtcccg gaggaagagc cgggcgcctc tttggggcac
      241 gcgggagcgt gccgcgcgcc tggcgcggtt gtccggtgcc tcgcttttgc cgctgtccgg
      301 cggctaccgg ttaccttgca gcgccttggc gacgctgccc agcctcaccc ggccgagtgt
      361 ccccgcgcgg cggcgcggac tgccggccag cagtgcggcc aggccgatgt gggcgaccat
      421 caccaggccg accgaggcca ggaagccggc cccgccggcc acgttgccgt cgtcaacgcc
      481 ccagaatatc ttcagccgcg cctgcacccg ctcgctggcg gtgcgtccct tgtacagccg
      541 ctcgaaggtg cgcgtggcgc gcgggatggg cgggaagcgg cgcaggtcga tcgactgctt
      601 cacgcgcacc gtcagcccgt agcgcttgtc cctgttgcac cggccgtggc acgggcagtc
      661 ccatttccgg tgcatcgccg ggcagcggta cttgagcgtg ccgcgcttcg cctcgtgccc
      721 gatgtaggcc atctcatgcc ggacgggcgg atcgctggcc atgtcgtagc agctgaccgt
      781 gccggcctcg tcgtagacga tgttgccgat gcccgcatcc tccagaaccc gctcggcctc
      841 gtccttccag agcgaccgcg tctcgatcag cggcttgatc ccgcggccgg ccagcaggcg
      901 gtgcgtcccc gtgtcgtccg ccgccatgtc gaacgccagc gtctccatgc gcccctccgg
      961 caggttgccg cgggccttct ccaccagttc ggggatcgac ttgctgtcgt gcgccgaggc
     1021 gggggtgaca cggtacgaca gggcgacctc gtggcgggtg tcgcagagca ggtgaagctt
     1081 gtagccgaac caatccagca ccttgacgca acggccgtcc ttgtcggtgt actccttgcg
     1141 cccgccgtcg ggctgcatct ccccgcgccc ggcgcgcgag cgccccctgc gggcgctcag
     1201 atgcgtcgag tcgccgctgg tgtggcgccc gagatccgga acggcctcgc ccaggcgccg
     1261 gaccatcgcg tcgaacacgc cggcgagcat cgcgcggcag ggctccctgc ccagcagggc
     1321 caggaaccgg ctcatgttcc agggcttggg cacctccgcg acggactcca tgccgcccag
     1381 cctccgcagg tccgcgttgc gcctcagctc gtcgagcgtc agctgcatcg tcggatggcg
     1441 cagcagcggc tggagcagcg cgcagaacca catcgtgccg acgggatagt cgtcgcggcc
     1501 gcgcccccgc cgcgcggcaa gggcggccag gagaggcccg tcgggcagct ggcggaagaa
     1561 gtcccgtatc gtctggaggt ccggcgagtc ctcaagacgg ttccagggaa agagggagtc
     1621 ggtctcgtac aaggtcatgc cgcccactat acaccgcaac cgatccccgc gtcaagcgct
     1681 atcttggcgg aaatggggta aaaaagcggt tttttccccc gccggaaaac agacccgcgt
     1741 gtttccaagg aaattcaaac atccgctggg ggatcgcgct tttccccgca cccgtttcgc
     1801 gcaatacagg tgcgcaaaac ttgaacagcg caatcggctc ttaaaaagta tacacgtatc
     1861 tgctcttacg tcaaacgaag acgacaccgg aatccggtgt cgtttcgttt gacgtaagag
     1921 gcgttctttt aaggcttgga actccctgat cgggaactcg gcctggccgg tcagtacacc
     1981 cattcgcgga tgcgccggac ttcccagtgt ccgggttgcc actgtttgca gcggcgtccg
     2041 aacgtgtcca gcgtctcgat ccagcaccct tcgacccaat agcgttcttc acgctcctgc
     2101 caatagccgc tgcggaccat caccggagcg ggcgccacca cgaccggcgg cggcacgaca
     2161 accacgggcg gcggcagaac gccgatgggc cgcggtcgcg ggagagggtg acgaggatgg
     2221 aaagcgcgcg gccggtggaa atgcccatgc ccgccctgat tgatacccaa ggtcaggaag
     2281 ccgttgccgg tgctcaatcc cactccgaca ctcacgcggt cagcccgcgc cggaacagtc
     2341 gccagcatgc ccgccatcag aaccgtcccc caaacccata tgcgctgtgt gttgttcatg
     2401 tccccacctt tctgacgcct gcgggggttc cccccgccga atgtctcaaa cagttgtatt
     2461 aaacgacggt ttaacttccg tattgccgtc acagcgaaaa aaagtcagat ccctccggga
     2521 ttgcccgcgc ggtccttttg tggcttaata cgaccaaagc aaaggatacg gaaacatggc
     2581 ggaactgatt gtggcgttgg atgtcgcctc tacagcggag gtggcccgga cggttgacct
     2641 gctgggtgat gccgtctcgt tttacaagat cgggctggaa ctgttcagtg ccgaggggcc
     2701 ggaggtggtc cgggcggtca ggaaacgcgg caagcgggtt tttctcgacc tcaaactgca
     2761 cgacatcccg cgcaccgtcg aacgtgcggt gcgggccggc gcggcgttgg gcgtagaact
     2821 gatgaccctg catgcggcgg gcgggcgggc gatgattcgc gccgcgaaag agggcgcccg
     2881 gagtgcgggc gcggatgcgc cgcggctgtt ggcggtcacc atgctgacca gtcttgatca
     2941 ggcggacctc gcggatttcg gtgtggcgcg gagcatggcc gaacaggtcg aggcgctggg
     3001 gcgtctcgcc tgtgagagcg gtgccgacgg cattgtctgc tcgcctcaag aggtggcgcg
     3061 catgcgcacg gtgctgggcg cggacgcgct cgtggtcacg cccggtgtgc gcccggccgg
     3121 agcggagatc ggcgaccaaa aacgcgtggc cacacctgcc gcggcagtgc gaaacggttc
     3181 gacgcatctg gtggtgggcc gtccgatcct tgaagcgtcc gacccgtgcg cggcggcgcg
     3241 ggcgatcgtc gcggagatga gcgcggccgc gtatgagtgc gggggcgtgt agcgggtggc
     3301 tggacagcgc gcccgaacag gagccgcgaa gccttcctca gattaaggcg tcgcggcttc
     3361 tgcgccgttt ccgtcgtcgg catccccttc gccggtcgca tcgagaatgc gcgcgacctc
     3421 gtcatggagc ggatgcccct tgtcccgaag cagagccttc atcatcggca ccttgatctc
     3481 ttcgggccga ttcagggcgt cgttaaagat ctcgtccagc acatcggggt tttgggtttt
     3541 atcgaaaaga atggcgaaca gggagacgaa ctgttcatcc ggcagcaggt tgagggcatt
     3601 ttggatggcg tccacctgat cgtccgcatg caggcgcatg aactcgtttt tgaattgctc
     3661 catctggccg gcctccggcg gggtctcgcg ctccgtgatt tggtccacga acgcctccca
     3721 cttcttgact gcctgttcgc ggggagcaga gcgcgcgacg gtcgaacttg agaccgcagg
     3781 cacggtcgac tgcgccgcgg cctccgcaac cgcagcggtg cctgcggcgg acggtgccgc
     3841 aacatctttc cttgcggcac gcgacggatt gcggcggaca gtgggcgggc gcgggcgcgg
     3901 gcgagcgcgt gccggttgcg cggcggaacc ggcggaggga gtcgcaacct gaccggatgg
     3961 cggaattttg acaggcatgt ccgaaacacc accgtcttga cgcaccgtgc ggttgcgcca
     4021 acaaagagag gcgaccaaaa tcgtgctaac cgctagaaag acgagcctcg tttgcgtcca
     4081 tcgagtcata tccgtatggt actctgatcc ggtgtgtggt gacaagcggg tttatccgca
     4141 gtttcaaaag accgccgcgt ttccgcaacg tccgttattc accgcatacg gcgagacgga
     4201 gcagagcgcg ggttaacgag gggtggcgcg acacaagcga aatgcgatca ctgcggacga
     4261 cgagcatccc gcgagccagc agcttatgaa aatgacggtt ggcggcaggc atactgatcc
     4321 cgcacgccgt cgacagcgtt tcaagggaaa gcggcgcacg aagatgcagg caacgcaaca
     4381 tggccagacg tcgcggatgg gtgaacgcgg tgaccacccg gataatctcc gcatcggaca
     4441 ttccgccaga acgcaacgca cggcggagag cggtcagcag cggtttcgcg gaagggacgg
     4501 acgggtcggg ctccggcaca taacgcaccc aacgcgatat gcgcacagcg gcgatcagac
     4561 cacgcgcctg caacgcccga agcgccagac tgacctgcgc ttcgtcgaac cgcacttcgc
     4621 gcgcgatttc ctgcacgcac aaattcggcg tgcccatgac cacccgaagg caggccaacc
     4681 gccttgcgtt cgccaaaacc cggcaagtcc gccacaatgt aggatgatag gttctgtcca
     4741 gcgtcatttc acacctttca aggtgtgaaa aggatacggc tggcattgac gcaaatcaag
     4801 tgtgatctcg tctgagaccc tttgtcgtcc aaatggtaaa gcggcgatca gaccacgcgc
     4861 ctgcaacgcc cgaagcgcca gactgacctg cgcttcgtcg aaccgcactt cgcgcgcgat
     4921 ttcctgcacg cacaaattcg gcgtgcccat gaccacccga aggcaggcca accgccttgc
     4981 gttcgccaaa acccggcaag tccgccacaa cgctggatga taggttctgt ccggcgtcat
     5041 ttcacacctt gaaaggtgtg aaaaggatac ggctggcatt gacgcaaatc aagtgtgttc
     5101 tcgtctgaga accctttgtc gtccaagtgg taaaatggtt tttcatgtcc actttcaacg
     5161 ggcgccccca atcctgatgc gggcagagcc cgcaaaatcc gcgcgactgg aaaggccaaa
     5221 ctcctaatac ggacagagcc cgcaaaatcc gcgcgactgg aaaggccaaa ctcctaatac
     5281 gggcagagcc cgcaaaatcc gaacgactag aaaggccaaa ctcctaatac gggcaaccca
     5341 atttagcctc acgcaaaggc tcaacagaca caaaggggtt tccataacac cccattcccc
     5401 ttcgtgctct tcgcatcctt cgcttcgtgt gagaaacaac ttgtttttta ttggccttat
     5461 cggccgacaa ggcaccaaat ggcgtccgta actccaactg gtgtgcgcca gcggcacggc
     5521 tgcaaaaccg aaacccgcaa tgattttctc gaccgcctcc tgctgacaag ctttccgcag
     5581 cctcaaaagg ccagatcaaa gatcaggggc agatggtccg acccgatgtc cgggccgcgg
     5641 cgccgattta tgatccgcac atcggatgaa tgcaggccgt gatccagcgg aatgcggagg
     5701 aaaggcagag aggcgggcca agtggggagc ggaccgcggc cttgggcgct gtcaatcaga
     5761 ccgctctcac gcaggaaggt tcggaagtgt gcggaccagg gagtgttgtt gaagtcaccg
     5821 gtcacgacca gcggagttcc caaactccgg aggtgttgcg caagcatccg catgaaacgg
     5881 ttacgtcccg cccacatggc ggcgttgtac ggggcgagcg cgtgcgtgcc gattacggtg
     5941 agggtccggt caccgatacg gatacgtgcg caggtggagg gtgcgccctc cgggtcatcc
     6001 agctggaaga ttccggcttc aagcgcgttc gtccgacagt agatcgccgc gccgaaatga
     6061 tcctcgcgcg gcatggacgc gtaaaccgga taacggttgg tgagcgacgc gagatcgcgg
     6121 agccagcggg tgttgggttc ctgcagaatc accacatcgg gctgttcccg ttcaacgagg
     6181 cgcaacagag ccgaggcgtc ggggttgtga gtgaggatat tggcctgaag caaccggagc
     6241 ctcacggccg gttgggggga gcggggtagg gaaacgggtg ccgcaggcaa cagaagcagt
     6301 agcacaggca gggcgttgac gccagctagc agaagaccag ccgccgcacg ccggtagcgg
     6361 cgcgcgaggc actcgaatgc cgcatatccg agaaagcaga gagcaaactg cggtttgaag
     6421 tgagtgaaga tctcgaaaag ccatccgaag cggccaaaga gtcccgccag agtgaaaagc
     6481 agaccgagaa aggcggcggc ttcgacaagg ccgagcaggc gtcggatgat cggcgggatg
     6541 ttcatgaagc gggcggagcg gggtttcgca attcagcccg gagcaggccg ttgtggcgca
     6601 gaaggggctc gatcgacgga tcccggccgc ggaagaggcg gaaaacttcg gccggatcgg
     6661 tcccgccgcc cagcgccagc agagagtcgc ggaagcgccg ccccatggcg cggaccgcat
     6721 cgccctgcgc gagtcccgcc tcctcaaaag cggcgaaggc gtcggcggaa aagacctcgg
     6781 accatttgta actgtagtat ccggccgcat acccgcccgc gaaaatatgg ctgaaggaac
     6841 agaggaaccg gtcttccggc agcagcgggg tcggagagta gcgcgcggca ttcaggcgtt
     6901 tgacggtgtc agcgtcaggc cactccggac gcggatatcg cgcgtacaga tccatgtctg
     6961 tggcagcgaa aaagagctgg cgcatcatcg cgctggcagc gcggtggttt ttcgccgcga
     7021 tcagccggtc aaacagcgcg tcgggcaaag ggtccccggt ctcggcatgg cgactcaacc
     7081 ccttcagggt ggcacggtcg taacaccagt tctccatgaa ctggctgacg atctcgatcg
     7141 cgtcccactc gatgccgttc agtcccgagg cacccggttc ttccacggtg gtgagcatgt
     7201 gctgcaacgc atgcccgaat tcgtggaaga gcgtgccgac ctcctgaaaa cgcatgaccg
     7261 gcggctgatt ccccaccggc acactctggt tgcagaccag cagggccatg ggcagggtga
     7321 cggtaccatc cggcgcgcgg tcccgcgtcc ggaaatcgtt catccacgcg ccgccgcttt
     7381 tggtttccgg acggctgtag gggtccagaa agaaacaggc gagcggagtc ccgtccgcat
     7441 cggcgacccg gaaaaaacgc acgtcaggat gccacaccgg cgcctcgccg tcggcgggcg
     7501 tgatgacgat gccgaagagc cggttcgcaa ggctgaagag cccttccaga acacgcggga
     7561 agagaaagta acggctcagt tcctcgtcgc tgtaatcata gaggtgttcg cgctggcgct
     7621 cggaccagaa ggcgatgtcc cacggttgca gcgcgccctc gtcgaacccg tggctgcggg
     7681 cgaaggttag cagttcttcc gactcggtgc gggccgcttc accggcggct tccgcgaggc
     7741 gggcgatgag cgtatcgacc gcagcgaccg agcgcgcggt tttatcggcg aggttcaact
     7801 cggcgtaaga ttcgaatccg agcagaccgg ccaattcgcg ccgcagggca agaagacgct
     7861 cgatcttcag cgtgttatcc gtggggccgg aagcggcgcg ggtggcatag gcgcggcaga
     7921 gttgttcgcg tgccgtgcgg ttacggctat gcatcatgaa cggcaggtat acagcggtgt
     7981 cgagggtgat gcgccacggg ccggtggcgg cggtggcgtc tggttcaccc gccgcgcgcg
     8041 cggcctgggc cgtgacctcc cggagtgcgg gagggaggcc agcggcctcc tccggtgtgc
     8101 gcagcgtgag agcgaacgcg cgggtggcgt caagaacatg gttgcgaaag gcggtggcga
     8161 gttgcgccaa ctcgttctgg atggcgttga accgtgtctg ccgttccgcg tcaagcccca
     8221 cgccagcgtg ttcgacggcc tgaatggttt tgacaagaat ccgccgccgg actggcgtga
     8281 ggcagggaga ggcgcggtcg gcctcgcgga gggcaagata tccccgatac agcagcggac
     8341 tctgtgcgac gcgctgcgaa aaggcgatga tctcgggctg aaggcgttct tgaacctgac
     8401 gccaggcgtc gctgttcatc acagaaagca tgtggctaag caacccccat gcgtcgaaaa
     8461 gcgggcggca ggcgtcatat aaaggccgca tcagtccttc ccacgacggg gtaagcgcct
     8521 gctccacctt tgtgactgcg gcatgagcgt cggccaacag atgcgggaaa tcagaagcgg
     8581 cggcctctgg ggtcaggcgg tcgaagggtg gcagataggg aatatggagg aaagcggaca
     8641 taacaagcgt gtgcgtgcga aaggggcccg gttccgcagg ggttaaggag tggtcggttt
     8701 cggcgcgacc ccgtgccgcc gcagtttctc ggtcacggcg ggcgtcggat cgatctcatg
     8761 actgcgcgtc cagaaagcag cggcttccgc cgaacgtccg accgcctgaa ggacatcgcc
     8821 ggtgtggtcg agaatgaccg gttcgctgcg gtcgaggtct gcggccttca gcagaaactg
     8881 gagcgcgtca taggggcggc ttttcttaaa aagaatccag cctttggtgt ccagataggc
     8941 ggcgttgtcg ggtgcggcct gcagggcttc ggtcaccagc cggtcggctt catccagatg
     9001 gatgtcctgc tcggcccaca ggtaggccag aaaattctgg agcgcgtccg aagtggggtg
     9061 cacggtgagc gcctcacgca ggatccgttc cgtccccgcc gcatcctttt ccgcctcggt
     9121 caatccggcg agccagagat aaaaacgctc gtccggaaag tatccggcgc acaaacgacg
     9181 ggtgtagtgt tcgcgcaggg tggcgatggc cgccggccgg ttgttctgtt cctgatgaac
     9241 tgcggcgagg atcaggcgtg tgggagcggt gtcgggttcc cggagcgcgc gtgcggccag
     9301 acgattggtc gcatccggat ccgcagccca gatcgcgccg aggcgcagca ccggaccggt
     9361 ggcgccggga tcctcccggc aggcggcgtg caggctggcg agggcgtcat tggtttgagc
     9421 gaggctgaaa tggcattctg ccgcgacagc gtgcaggccg ccgcgccaca cgggggtctc
     9481 ctcgtccgct tgcgcgaggg cgagatcgac gcaacggagc gcgcgtgcgg ggttgctctc
     9541 ctgttcccgc agaaagtgaa ccgcccagtg tgccggaagc accgcactgc cgcgatcgtg
     9601 aaaacgcgcg tgacaggcgc gcatggcttc gagcgcttca ttctcgcaat cggccatgaa
     9661 atagagtcgg atgcgcgcca gagccaactc ccggttgtcc ggctcaatct ccatcagaag
     9721 cgagcagtgg cgggccgcct ccaacgggcg ggcggccgcc tcagccagac gcaccgcctc
     9781 aatacggata gccgtgtcgt ccggatgacg cgtgaggaaa ttctcagccg cagcgaatcc
     9841 ctcggcaaat tgttcgcgtt cggcgagggc gctcagcagg acgtccagcg gttcgcggcc
     9901 gtcggggtcg agccggaccg cctgacggaa agcggtcagg gccgcagcct gagccttggc
     9961 gcgattggtt tcggcacctt cgccgctctc gacaagcagc ccttgggcca tcaacgccaa
    10021 agcgccagcc ctacgggcgt cggcctcttc agaagtggag gggtctgttt gcgaggcgtg
    10081 cggcgcgccg cttatcagac aaccggccag caacggcaac aacgccgcac cgagaaccgc
    10141 acaccccaaa ccgatcagcg gacgcagggc aataagctca atcggtttca tgtcggggtc
    10201 tccaatacac atcacacgcc acaccccttt cctttccggg aaacggggag aaagggcacg
    10261 gctatcccat ccgcaggcgg tcacgcgccg gtgtgcgcct cggcgcctta cttgttcttg
    10321 tggcgattgc tcttcaggcg tttacggtgc ttatgcttcg acatcttcgc acggcgcttt
    10381 ttcttgatcg aacccacgtt ctttctcctg gaagtgcagg gcgtacccga tggggtgtcc
    10441 tgttgttttg ttcaaaaaat gactttgttc agcggcagct caatgatccc ggtcgcgcca
    10501 gcgttccgca acggcgggac gatgtcgcgc acgacctgtt cctcgaccac cgactcgacc
    10561 gcataccaat cacgcttgtt gagccggttg accgtagggg catgcagcga cggcagcagt
    10621 tcggtcacgg tgtcaagccg gtcggccggc acattcaact tgatcagcac acgcgactca
    10681 gccgccagcg caccctgcaa cagcacggcg agttgttcga tcttttcgcg cttggccgga
    10741 tttttccacg ccttgtggtt ggcgatcagc cgggtggtcg aggtgagaat ggtgtccaca
    10801 atccgcaggt tgttggcacg cagtgaggag cctgtctccg tcagctcgac gatggcgtcc
    10861 accagttccg gcgccttgac ctcggtggca ccccatgaga actcaacctc ggcctcgacc
    10921 tgatgctggg cgaggtaccg ccgcgtcaga ccgaccgcct cggtggcgat gcgtttgccc
    10981 tgcaagtctt tgacactctg gatcggcgag tcgttgggca cggccagaac ccagcgcacg
    11041 gactgcgagg tggccttgga gtagcacatc tccgaaacct cgtgcacatc ggagttgttt
    11101 tcgtaaatcc aatcatatcc ggtgatgccc gcatccagaa gcccgagttc cacgtagcgc
    11161 gaaatctcct gagggcggat cagccgacac tgcaacccgg ggtcgttcac gctgggtgaa
    11221 taagaccgcg atccggcctg aacgacaaat ccggcctttc gcatcatttt gaaagttgct
    11281 tcctgcaaac ttcccttggg caatcccaaa ataacagcca taacgccttt ggtctctttc
    11341 cgattggttt gataaaaggt taaacatctt atcggattaa cctaaagtag gcaagtaccg
    11401 cgaaatcgat ttttgaaccg ccgatcgttc ggtggtgggc gaggcgggac tcgaacccgc
    11461 ataccttacg gcacaagatc ctaagtcttg cgtgtctgcc aattccacca ctcgcccgca
    11521 cagaggaccg caacgcaccc cgtcagcagt ccagatgccg cattgccgga aaccgcgtca
    11581 cggtgcgaac aacacactgg tcagcccggc caactcttcc gccgcgatct tcatgacctt
    11641 gcggtcatag gtcatcaaac cgttgacctc cccttcgaca tccgtggtct gcgtgtagac
    11701 gccgcccgcg atgccttttt gtcgcagctc gttcagcagt gtgatggatt tcgcgacgcg
    11761 ctctttgtac tcggctttgt tcttcggcaa accgccgtag ccccagttct cgcgttcgtc
    11821 atcccacaaa tgtcctttcg ccagatatcc atgtccgccg aactccccga ccaccttaat
    11881 gaaggaatca aaacgaccgt cttttcccat ctcgaacgga aaagcggggt gcggatagct
    11941 gtgggcgtcc acaacgtccc ccaccggcca gaagttcccg ccgctggcga tattcacgag
    12001 acgcgtcgga tcgcgccgcg cgacccacgc cccgacatca agcgttcggt gctgacccca
    12061 cgcctcgttg aaggggaccc aagtgacgat gcaggggtga ttttccaacg tgtcgatcat
    12121 ccgccctaac tccagcatga actgcgcatg atgtgcttcc ggccactctg cgtcccgggg
    12181 ttcgggtttg agaaaagtcc actttgcgcc ttttccgccg ctcacctgat cctgccacac
    12241 catcatgccg atgcggtcgc aatggtaata atagaggcgc ggctcgacct tgatgtgttt
    12301 gcggatcatg ttgaatccgg cacgcttcag ccagtcgatc tcgaaggcca tcgcctcttc
    12361 ggaaggcggc gtcagcaacc cgtccggcca ccacccctga tccagcggcc cccagtggaa
    12421 aatcgtctca ccgttcagag tgaagcgcag gtgcccttgg gcgtcgcggg tcttgcccac
    12481 cgtgcggatc ccggcgtacg actcgacctg atccaaaacc ttcccgtcct tgtccagcag
    12541 ggtcagcttg atgtcataaa gatgcggcga ggcaggcgac cagcatttgg ccttttcaac
    12601 ccggaccgag atcggcgcgc ccgccttacc ggcaacctcg gccacccgct tcgcgttctc
    12661 cagcacctcg acacgcagtg tctctgccgt gccggtaacc ttgggcgtca cagtgatcgt
    12721 gcctgtcgcg gcatcggttc tgagcgcgaa gtcggcgaga tacgtgcggc cgacctgctc
    12781 cagccacacg gtctgccaga tgccggagac ttgcgtatac cagatgccat gcggcatcag
    12841 ggtttgtttg ccgcgcagtt ggaacccctc ttgctcgtcc tccacgcgca ccagcaattc
    12901 gttctcccca acgcgcaccg catccgtgat gtccagcgtg aacggcgtga atccgccctg
    12961 atgtccgccc acgtcccggc cattcacaaa gacccgacag cggtaatcca cggcttcgaa
    13021 gttcagcagc gtgcgttcgg ctgccgccac ggtaaacgtg cgccggtacc agagcgcctg
    13081 atcctgccgc aacatcttct gcacgccact gagtttcgat tccagacaga acggcacccg
    13141 gatgcggccc tgccactgga gcggcggctc ttgccgcgcc accggcgtca cggccatttc
    13201 ccaggaaccg ttcaggttct gccacgcttt ccgccgcagt tgcggccggg gataacccgc
    13261 ccacgcgttt tgatccgtca ccttctcgcc ccaaggcgtg atcagcgcac tcgagaacag
    13321 ttcgcgttcc ggaatcggca gttcaggcac ggcggcggca tcgatcacat gcgcgtcaat
    13381 gaactgtccg ccggtcgttt ggcggcaatg cacggccagc aggttctccc ccggcctgag
    13441 cagggcacgc tgcgccgcat caagaggaaa gaccttgtac tccttgagga atccgttgag
    13501 cgacagcacg ggctgtccgt tgagaaaaat ctgcgcatcc tcgtcatgat ggaccaggag
    13561 cgcgggctcc gccacatcgc gcgcgaacgt gaaacgcttg cgcagccaga tcgtccgggt
    13621 ccgccactgg gtccccacac gcgcacccgg cgtcccggcc gtgccaaacc cgcccgagcc
    13681 ttttttccaa gccgtgtcat caaaatccgg cgcctgccat gccccttccg gctcctgcag
    13741 cgtataccgc cactgatccg tgatccgggc cgtcagcatc gcgggaaacg ccgcgcagag
    13801 cgcaacgatc ccaatgaacg tgaaattcaa acgcatgttt ccctcccttt cctttttcgt
    13861 acagaccgac ccgcgccacc ggtcagacat caaggttgcg cacgttgagc gcgttgtcct
    13921 cgatgaactt gcggcggggc tcgacctcgt cgcccatcag cagggtgaag atcgcatcgg
    13981 ccttcaccgc atcctccagc cggacgcgca acagacggcg tttcgccggg tccatggtcg
    14041 tatcgtaaag ttgttccggc ttcatttcgc cgagaccttt gtaccgctgg atcgaaagcc
    14101 ctttacgtcc gcgctcgcgc accgtgttca gtagttcggg aagcgcgcga accggaatag
    14161 actcgtcctc ttcctcaata aacacagccg gagcgtcgcc ccccgcgacc tgttcgacgg
    14221 tgaagcccgc gccgaccagc gagtcgatct gcttcttgag aagatgcgcc cggtacatct
    14281 cgagccagcg gaacggcatc ggctgtgtcg ggtggtacgc ctgcacctgc gtctccgtca
    14341 aatcgatcgc cgcgcccagt ttgctttcgg cctcctgccg cgcattcgcc agatccgcat
    14401 cgctgtacac gaagcggaac acggcatcct ccccactgcc gaggatggtc gcaaaccgcg
    14461 gaaactcgcc ggtctcgggt ttgcgcaggc tcagaaacgt gtccggatcg atcccgcggc
    14521 gggccacagc ccggagagtg ttttcgatct gggccagcag ttcgagcagg cggcgcaact
    14581 ccttcgcgtc aaggacccgt ccttccggag tgcggaccgt catatcgtcc gcacccaaaa
    14641 gcagcagttt gctggtgagt tggtcgtcgc tctcgatgta ctcctcgcgc tgcttgcgcg
    14701 tgagtttgta aagaggcggc tgggccaggt agatataccc gttctcaatc agcttcggca
    14761 tctgccggaa gaagaaggtc aggagcaggg tccggatatg cgcgccgtcc acgtccgcat
    14821 cggtcatgat gatgatcttg tgatagcgga ctttggagat gtcgaactcg tcggcaccga
    14881 accccgcgcc gatggcggtg atcagggtcc ggatctcgtt gttgttgaga atcttatcga
    14941 tccgggcctt ctcgacattc agaaccttgc cgcgcaacgg caggatcgcc tgagtctcgc
    15001 ggttacggcc ctgtttggca gaaccgccgg ccgaatctcc ttccacgata tacagctcgc
    15061 acttggcagg gtcgcgctcg gaacagtctg ccagcttgcc gggcagggcc aggctgtcga
    15121 gcacgccctt gcggcgggtg agatcccgcg ccttgcgcgc cgcaatccgg gcgcgtgcgg
    15181 cgagcacggc cttctcgacc actttgcggg ccacagccgg cttctccccg aaataggtgc
    15241 cgagttgatc gttgacgatc tgcgccacga tgccctcgac ctcgccgttg ccgagtttgg
    15301 tcttggtctg gccctcgaac tggggttgcg gaaccttgac ggaaaccaca accgtgatgc
    15361 cctcgcggat gtcatcgccg gtgagcgcct cctcattgct cttctgcagg ttgttgtcgg
    15421 caatgtactt gttgacggta cgggtcaggg ccgaacggaa gccggagagg tgggtgccgc
    15481 cctcgatggt gttgatgttg ttgcagtaac tgtattcgat ttcgttgtac gaatcggtgt
    15541 actgcatcgc gatttcgcat tcgacccggt cgcgcgtgcc ggaaaagtag atcacctcgt
    15601 catgaaccgg cgtcttgttc cggttcagga actggacgta ctccacgatc ccgccgttga
    15661 acaggaacga ctcgtcccgc tcctgctcgt gctccatgtc gcggaaccgg atggagaccc
    15721 cgcggttgag aaaggccagc tcccgcaggc ggttggcgag gatgtcccat ttgaattcgt
    15781 ggcaggtgaa gatcgtgtgg tccggataga acgtcacctt ggtgccggtg ccgtgcgtct
    15841 cgcccaccac ctcaagcttc gtgaccggat gcccccgctc aaagcgctgg cggtagatcc
    15901 ggttattgcg gcggacctcg acctccatcc actcgctgag ggcattcacg caagagacgc
    15961 ccaccccgtg caaccctccg gagaccttgt agttcgagcc gtcgaacttg ccgcccgcat
    16021 gcaaagtggt cagcaccacc tccacggccg gacgtttttc aaccgggtgc aaatccaccg
    16081 ggatgccgcg cccgttgtcc acaacggaaa gggaaccgtc cgggttgatg gtgacatcga
    16141 tatgcgtgca gtaccctgcc agcgcctcat cgatcgaatt gtccaccacc tcatacacca
    16201 aatggtgata gccgcgttcc ccggtgtccc cgacatacat ggcgggacgc ttgcgtaccg
    16261 ccttcattcc ttccagcacc gtgatattgg ctgcggtgta cttttctgtg tgcgtttctg
    16321 cctgtgcggt gtcgagttgg gaactgcttc gcgtatcgtc tgacatacct ttttccgtgg
    16381 ttggtgagaa tgaataaagg ataccttaaa aaggcgtccg acacaagcaa atgaccccgt
    16441 tttttttctt taaaaacaag ctgttttcac cccccgcccc cagggagaag gccggggtga
    16501 gggttgcggg ctccgcccgc gtcaagacgg ctggctgtcg ctgtggagtg cgtcatccgg
    16561 ggtttaccgc acgccgcagt atgcggaaca gatgccgccc gtgtaacgct cgcaacgggt
    16621 ctcccggaac ccggcctcgc gcagcagatc gagcatcgcc ccgccttgcg gcacctgccg
    16681 gatggaggcg ggcaggtagg cgtattcgcg cgcgtggcca cccaagcagc gtcccatgac
    16741 cggaaccgcc acccgcagat agacgtcgtg cagaagacgc aacgggaacc gttccggccg
    16801 cgagagctcg aggaggagaa cctgtccgcc gggcttcagc acacgccgca tctcgcgaag
    16861 cccgtccgca agcgaggcga agttgcgcac gccaaaggcg gccgtgaccg tatcgaacga
    16921 ggcgtcgggg aaatccagtg ccagcgtgtc accccggtgg aggctcacac gcgcccccag
    16981 tccggcgcgc cgcacctttt cccgcccgag gtccagcatc gcttcggaca aatcgatacc
    17041 cgtgacacgc gtctgcggca gacggcgcgc caccgcgatc gcgaagtcgg cggtgccggt
    17101 ggccacgtcc agtacgcggg ccgacggcgc ggcgcgcaac aggcgcagcg cgcgccaccg
    17161 ccaaaaccgg tcgaggccca atgacagcag acggttgagc cggtcatatc cgggcgcgat
    17221 cgcgtcaaac gcgcgcgtga tctgctcgcg tttaccctcg ccggcgccat aaggggtgat
    17281 gtgttcaacc ttcatgcagt gtcttggcgt tgaggtcggc ggtgaggagc cgatcggctt
    17341 ccgccgcgcc caatccgcag gtttccgtca gctcttcacg cagatatccg gccagacgcg
    17401 gcataccgat cgcgaaaagc ctgccttcgc cagcatagag gcgcgcggcg agacgcgtgt
    17461 acgcgccgaa cagatcaggt ccgcaggccg catggaacgc tcggacggcg tgcgggaaac
    17521 ggccgcggtt gtggacccgt tcccagcagc gggcaaggcg ctgaatgcgg catagggtct
    17581 caaaatccat ggtgtccgag cgcaacagcc cgtaaggcgg ctccggactg aagacaaggc
    17641 cgaggctgtc ggcttcgcgg gcgaagcgtg tgccggggag tcctttgagc aggttgacct
    17701 gcacctcggg tggtgcgcag gttgtcacca gcgtgttgaa accgttggcg aaggaggctt
    17761 cgtcctcgcc gggcaggccg aaaatgagat cggcgtgcac gtcagcgccg gtttcgcggg
    17821 tgaggaaacg caggttggcg agtgtggtgg cggtgtcggg gctgcgtccg gcggcggcgg
    17881 cgacttgcgg attgagggtc tggatgcctg cctcgaggtg aagggtgcct gctggaaagc
    17941 gcgcgaggcg gtcggccaca tccggatgca ggcgttcggg gttgatctcg aggtgtagac
    18001 aggtgtcttt aggcgtgacg cgctcctgaa aaaagtccag taccgcgcag gcgtgggcta
    18061 caggcgcgtt gaaattgcgg tccagaaatt tgaagcgccg caacccgcgt tgccaaaggc
    18121 ggtcaaaggc gggcaagatg cggtcgagag ggagaagccg caggccgctg ccagccgatg
    18181 cgcaatatcc gcagttgtat ggacaaccgc ggctggtctc gacatagatc gtgcggcggc
    18241 tcagatcttc atcagtgtag aggtgatagg gaagggttaa ggagttggga attaggaatt
    18301 gggagttagg agttaggagt tgagaattgg gatctcgttg agagggggaa gttgagagtg
    18361 agggggcggg ggacgggatg gatgtttcgc agaatgcgcg gaaggcggct tcgccttcgc
    18421 caatgattac cgcgtcgaat aaattgacgt gggggtattc agaggtgatc tcagagccgc
    18481 ctgccacgag tttgagatcc ggcgctacca gccgcagaat gcgcgcggtc gactcgatta
    18541 agcgcacatt ccagaggtag acggagaaac tgacgatgcg cgggttgtgc gcggtgatgc
    18601 gttcggcaag ctgcaccggc gtgatctcca gatcgcactc cagtattgct gcttggcgct
    18661 ccagcgttcc gagattggcg agcagcgagc gcgctgcaaa agaacagtgg ctgtaacgcg
    18721 cgttgacggc caccagcagg atgtcacgat ggttgtgttc agacaaaagg ttcagtcctt
    18781 gagcaaacgt tcgacatgtc gaagttttta aacataaagg aaaggtgtgc ccgttcaggc
    18841 ctgaagggaa ttatagccgg cgttgtaaac cagcaggaca tcccccggca gcgcgacgat
    18901 ttttttcatg cgcgcggcgg ttgcggcttc attgtcttca tccggagggt cgaagcttgc
    18961 gaacaatacg gcggaaggct gcatggctgt acgcaccgca aaagtcgggt tcgcgcaagg
    19021 cagaatgacg atctcggctt cgatacgggc gccctcgagc atttcacgca tcgctgcgga
    19081 gatgctcatg acatcggctt tgtacgggac cgggcgcagg atgcggatgg gacgggtgcg
    19141 ccactcgctg ttctttttga gcagataggc cagcagcagc aagagggagg cgtcgcgctc
    19201 attgttccac cagatgttaa tcgcgccgtc ggcgatgacg gtgtgagcct ccttatcgag
    19261 cgctttgttg gcgacaatga cgatactgcg ctccatgcgc ttggccagcg ttaacagccc
    19321 gaagaacacc tctgactttt ccgcgtcctg gctccagccc agcaaaaggg tgttggggcg
    19381 caagccgccc agtccgtggc attgcagcat cccctgcaca ccggcgtgaa tgttcttatc
    19441 gacaaccaca gcgggaaaag cgttcagccc ttcttcgcgt atgaatttgc gcagcaaaaa
    19501 ctcggcttcg cgctggcggg cgacgagatt gtccagattg ccgcgtatga cttggccgat
    19561 cgaaacaatg ccgtggctgg cgctgagcca acaggcgtac tcggcgaggt ggatgcggtt
    19621 gtaggccccg ccggaaagcg cgagaatggt ggggcgccag tttttggggt gataacgttc
    19681 ttgttcgagg cgcaacaagg cattgcgggc acgctgatag gccaggccgc tttgcatgtc
    19741 gccccatttg accatgatct cgatgcgtcg gatcacgctg tacagcaaga gcgccaacaa
    19801 gatggcgatg aaggcccaga aagcgttgat caggaacatt acgacaaggc atccgaccgc
    19861 accggcaagc gcgatgatcc aatggttgat gcggaaggtc ggccggaaac tggggttgcg
    19921 cgagatgaac tcataaaagc aggccaagtt gacagtaccg taggtcatca ggaagaacat
    19981 ggtgatgacc ggtgcgacgg tgtcgaggtc accgaccatg atgccagctt gcgcgatcac
    20041 gaaagtcagt gcgatggcaa agcgcggttc gttgttttta ccgctgccgc ggccgagcca
    20101 cttgatgccg cggaaaacat catcgcgggc gaaagcctgc aggatgcgcg gcgcacccat
    20161 catactgccg agcgcggatg agagagtggc acagaagaca cccgcataaa tgagcgggct
    20221 gaaaagcgcg cgatctttca tcacaaagcc gtcgctgatc aattcagccc taggcacact
    20281 gccggccagc aagaaggtga ccagtgcgta aatcaaaccg gtggcaccga tggccgcgaa
    20341 tgtgccgcgc gggattgaac ggctggggtc ttttaggtcg ccggacatgt tgacacccgc
    20401 catgatgccg gtcacggcgg ggaaaaagag ggcgaagatc gtgaacaaag agtgtccgtc
    20461 cttccattca ggcgcaagat tggactgcag aatctctgga gaaaacccca gcgcgccgcc
    20521 gataaagaat gaaaggattg aaagcatcat gagggccagg atccagtact gtacgcggat
    20581 agtccaattg gcaccgatat ataccaccaa gaagacaatg aagttgctta aagtggcgac
    20641 gtcacggaaa gacagcggta tattgggaaa agcggaaaag acggcctcgg tgaaaccgat
    20701 gatgtacatg gtgacagcca cagtctgcgc gatatagaaa aagaccgcga tgacgccgcc
    20761 gaactctggc ccgaggctgc ggctgatcag gtaataggcg ccgcccccct tgacccgcat
    20821 gttggtggag atggacgaaa gcgaaagcgc ggtgatgacc gtgatggcct ttgaggcggc
    20881 caatatcagc aaggcgttcc acaggccggc atagccgacc accacgttga aacgcaggaa
    20941 catgatcacg ccgataatgg tcagcacgct cggcgtgaag acaccgccga aagtgccgaa
    21001 cgaatatccc tgcttggtgc cggatgagcg tttgcggtta tccatttaca aaactctgtc
    21061 tttacaacgc aacgtattcg tgttgcattg tccacatgtg taaacaatgc caaacctgat
    21121 acggtctcgt caaggagagg cgttgcggtt cattaaattt ttcgtgcgag aaaaaacacc
    21181 tgaacgttga gtgttgagcg ttgaacgttt agcaaaaacg ctcttgcatc acccccgtcc
    21241 tttcttcatt atatacccat aacggttttc ggatgatgtt tgcccgcatt caatccagcc
    21301 aagaggtcaa ccatgaacaa cgccgccctg tggatgaaac cagaaacccg aaaccagaaa
    21361 ccagaaaccc gaaaccagaa accaatctct gcgtttccgc gtctctgcgt gaaacggtcc
    21421 atgctcgcgc tcgcctgcgc gggcctgtcc gcggtcgccg cctccgccgg cgaggcggcg
    21481 gttccgatgt cgtcgccgga tacgaacccc tacatcacct tctcaagctc tttaaaattc
    21541 tatgtctggc cgcaaaaatc ctcggacggg acgctcgaat actccgacaa cgcgaagcaa
    21601 tggcttacga acaccaccgc caacatggaa gcggtaggcg actccggagt ctataagctg
    21661 tacgtccgtg gaacgggcaa cacccggatt acagccgata gtccccccca cggctggcaa
    21721 atcaccgccg ataatccgga cacccgggtc gcctgctccg gcaatctcgc gaccctgctc
    21781 gactataagg cagtgaaaaa cggccagcat ccgccgatgg atgaaaaatg cttcgcgcac
    21841 ctcttcgagg gctgtacgct actcagcagt gcgccgtcgc cgccggcggc gaatctgacg
    21901 tatgcctgct gccgcgccat gctcgaaggc tgcacgaacc tgacgaacgc gcccgcgctg
    21961 cccgccacga cgctggctaa cgcatgttac gacagcatgt tcaagggctg cacggcactg
    22021 acgcgcgcac cggagctgcc ggccacgaat ctggcgctcg gctgctacgc ctacatgttc
    22081 atcggctgcg ggagcctgac caaggcaccc gcgctgcccg ccacgacgct ggcacccgcc
    22141 tgctaccgcc aaatgttcgg ctcctgcggg agcctgacca aggcgcccgc gctaccggcc
    22201 atgacgctgg cgggatcctg ctacctgtac atgttctacg actgtccggg gctgacggaa
    22261 ttgccggtgc tcccggcgac gaatctggtc gcgaattgct acgacaaaat gttccagaga
    22321 tgctcgaaca tcaaagtgta caccacaggc cccggagtac aatggagcat cccgaacaac
    22381 gccaccacga cgtttgcaac gaactggaac tacaggatgt tcgacggcac gggcggcacc
    22441 ttcaaagacg ccccggagat cggcaccccc tactactaca cgctgcccgt gccgtttccg
    22501 gactcggacg cgtgcctcac cttctccagc gcgggcgcct tcacggtcgc gccgcaggag
    22561 gtctcgtggg acggctcgct ttacgcctcg accgacacga cgaattggat ggcctttgcc
    22621 accag
//
LOCUS       DAOSLE010000026        21843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2652_length_21843_cov_8.229071, whole genome shotgun sequence.
ACCESSION   DAOSLE010000026 DAOSLE010000000
VERSION     DAOSLE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21843
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2652_length_21843_cov_8.229071"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PK576_03340"
     CDS             complement(<1..226)
                     /locus_tag="PK576_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019603352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60654.1"
                     /translation="MGILDIVIFVGFIAAVLSVGLWKSKSVQSQESDAADYFLAGRGL
                     TWWLIGISLIAANISAEQFVGMSGQAASYLG"
     gene            complement(247..1338)
                     /locus_tag="PK576_03345"
     CDS             complement(247..1338)
                     /locus_tag="PK576_03345"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOE60655.1"
                     /translation="MRYTCEPSVVVKPFGKTKDGQLVQQYTLTGAGGVVMDVIDYGGK
                     VVRLFTPDRTGAKTDITLGFNDVAGYETHDPYFGSLIGRYGNRIANGKFVLDGKTYTL
                     PINNEPGGLPCSLHGGKRGFDAYVWDSEPLSDGKTIGVQFKMRSPDGDQGYPGNLDVT
                     VRYWLTPENVWRIEYEAVTDKATPVNLTQHLYFNFKGEAEGTILDHELTLLADRMTPV
                     NKGLIPTGEVVPVKGTPFDFTTPWLIGARIKADNEQLRFGGGYDHNWVLNDQSGKLAP
                     AAALYEKSTGRYMEVWTTEPAIQFYCGNFLTDQVPSKQPGKNLCHRGGLALETQHSPD
                     SPNQPAFPSTILRPGAIYKSTTEYRFGVR"
     gene            1703..1903
                     /locus_tag="PK576_03350"
     CDS             1703..1903
                     /locus_tag="PK576_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008276123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOE60656.1"
                     /translation="MATNLQLDDRLIAEAARLGKHRTKKEAVTQALTEYVRNLRQEGI
                     LDLFGSVDFDPSYDYKRQRSKS"
     gene            1900..2319
                     /locus_tag="PK576_03355"
     CDS             1900..2319
                     /locus_tag="PK576_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE60657.1"
                     /translation="MKVLVDTSIWSLALRRREHAQNPEAEELGRLIASHVVEIIGPIR
                     QEILSGVRDGAQFERLARHLDAFVDQPLTVEDYVTAAKFYNLCRSKGIQGSNTDFLIC
                     AVAVRHDFSIFTTDKDFLHFAKCLPIVLHDVGQLRDR"
     gene            complement(2399..3622)
                     /locus_tag="PK576_03360"
     CDS             complement(2399..3622)
                     /locus_tag="PK576_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOE60658.1"
                     /translation="MTRQLAVMLAVACLAGSGRSGDWSQWRGSNRDLIVTEERLLPEW
                     PVGGPQRLWHVELPGEGYSEPIVVGGTLYITGSSGEKKSRVGHLFALDPKSGAIRWQV
                     EYGPEWGASYEFARTSPTFHEGRLYLVGGLGHVVCIDAKKGDIVWRVDSHAEFSGRNI
                     TWGIADNPLIYDGKVICQPGGPDAAIVALDIKTGRAVWKSKGLSERSAYCSPALLTIN
                     GRRQVVTMLEDHVVGVDAATGQPLWRYPHRNKYAVHPNTPVLCGKDRVFISSGYVLGS
                     AILEIAGGTVKQIWADKSSDNHFQGVAFYKGRIFSSGGGRLTCFDPADGRAVYHVEGA
                     KKTSFCITPVGMITYDERGGTVMLVEVGADSHRVISSFKIEYGNGPHWSSPVVSDGVL
                     YLRRGKGLAAFAVGD"
     gene            3757..8709
                     /locus_tag="PK576_03365"
     CDS             3757..8709
                     /locus_tag="PK576_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOE60659.1"
                     /translation="MTVARNIVWHVAACVFALGARVQAADPGEACATQTLTFALPRVC
                     ITADGRTRVGLAGCESDAVTGLPVLPVAGVTFSVPPGMAVDRVQVLHDAVTEIPLDAP
                     VEWGQPPPRIGEMPFPEAPPDPAVYGGTHPYPDPEQTVWRVDPNGADAARLSVRVCPV
                     RYDPQRSLLLAAASVTVTVEFRALRPTGIAVRSAATATSELNEMPGPFTYVIVSTSNL
                     IHNTPAPWNFAALCAARARTGFTTTCVSAEWIDSRYAGENRADRIRAFVQDAHRNWGT
                     RFLLIGGTFDLIPVRKLRVAVNEIIVTRIADIPADALYYGCLDGPFDGNGNGLFGELQ
                     DGANGGDIDLLAEVYVGRFPVANPEELSRMIRKTLRYEAASAEELAPCAFLAEKMDLG
                     LMTYTGGFMEELRFGSTYDGLTTIGFETSAFAPALDTGHTLYDSDAGLWSTEHAFAFL
                     NRNFGILNHIGHAVAKQCMKINLYTSAHQAALQACTNTLPYFVYSQSCLAGAFDTPDC
                     FAEQLVTVSNAAFAAVMNAREGWVYNHVVGGFSHRFHRSFWDAAMRGNGTRLGEIGEC
                     ARRMNLHLVSPSYGTYWRWVFYELTLFGDPATPFAARVNPTPPVFDHPGLINTYETQT
                     AHRVACSLEPIGLYDPASVRLLWRTGSDPETVHTQFLANVSGNRYETTIPAHPAGTAI
                     RYALFAETLAGVTGRWPLEEPEHLFHITDRLDLEIRGTPAAIGTPDPGYGTTHYASGL
                     VAVASAPEHVAVTEETRHTCIGFTGGGSTPPAGDQPDVAFRIDTSSHLTWIWQREHLF
                     SVTSALSEFPHTAFWVPENEASPVPPAPATLFVDGVLYAFAEWWCNDQRLPPAPAHSP
                     HDLGAIIADQPRRFTARYLPIEQDTDGNGIPDWWETHYYGHSGRLPDSDDDQDGYTLA
                     EEYTDRTDPLRATSVPAPPVITHEPLAARQTHPAPFIVQARITDTHCVTSAVLYWCHN
                     TNLWQTVPMTAGSNDLFFAEIHAECAPGDTFEYRIAATDPTGRTAESGPYPFALFYPL
                     IDTVQLHDLMIVAQPTQTPFTVRMDLANRGNAELYWRVQPARIERILSERLPSWDFTS
                     LGQPWSVSTNRAFSPPYALHSRLLSNAQPRTPVRASLTFPAVTLEPGATLSFRYWIFS
                     EVHQNTTRAFDGGLVEISTDGGETFMLLRGPYTHTIYGWNASPWPEGTPCFAGKGTEG
                     WQTAVFDLAAHHPEANGFAGSNVVFRFHYGGDNNTDNEGWYLDDIAVSPIRWPAGFNF
                     YGAAPETRTLAPGAHYRLLWSTDPSAMTMRDNAVDILFDSNEPVHQDFSVRWQVILRE
                     FPNLPGFNAVQTSSGDGRVLLETGLADRDGEAVSLEVAWSPDNGGHWQPAALHDVTVS
                     EGDAADDAPDGCLAALPTSRAGQPVTNLFRAFWSSREAVPPVGVNTQTRFRIVADNGF
                     FQVTALTQRVTVDNLPPRFLPGMLSAAPTSPQGGYAVTENLLTLHWPAAEDEPADSLL
                     RYRLTSQCGTSILEQTSHTLALSNALDQSHAFQVVAFDPAGNTSVPLDATLLVLNAFS
                     DYDGDGVTTADEELAGTRADDPSDRFTVRLRESADALTLSWPGVVGRLYTIETTPSLA
                     PAVWQPLAGHIDIPGTGAMHDAVLPRTTPTSFYRIRITLP"
     gene            8706..9797
                     /locus_tag="PK576_03370"
     CDS             8706..9797
                     /locus_tag="PK576_03370"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOE60660.1"
                     /translation="MTTSFPFPFRPVDAPYFRIAVLCAVLAGGCSRQPAAPLRIEWLC
                     MGTVAAVQCADRSRATDARDQAQAVFAELDREFSAWHGESVLSAVNRAAGSGQATAVS
                     ACFATVLQTALDASEASGGAFNPMIGPVMQAWGFNGAMQPPEYPDDRILNAAFAMADW
                     RAVICNKTTDGAEVRLPVAGMSLDLGAIAKGYAVDLAWERLKAAGHTNLLIDLGGNLR
                     ALGEAAPGRGGWRTGVRNPFQSNTMVGKFLMRHGEAVATSGNYERFVEIQGLRYAHIL
                     DGRTGLPVTGMASVTVIAPDAMTADLLSTTLFILGPEAGVRLLSRYPGCEALWIPDTP
                     DQLTLLSTPGFADRLTPHGKTPYALSVIR"
     gene            complement(9839..11656)
                     /locus_tag="PK576_03375"
     CDS             complement(9839..11656)
                     /locus_tag="PK576_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE60661.1"
                     /translation="MARIAARWIGVAVALVYVQATAAETPLTPVDVVADRAGKTLYVA
                     CETAGKVLVFDVESEKVTARYAVEGVARLAVADEQDRIYATAGGARGLLHELDRKSGR
                     ILRTLPAGHTPEALCLSADGKTLYYCNRFARADQPNVHAVDLASWKVRNTAKAVREPC
                     ASCLSDDGSTLWVGNYLPLMPANAQHVYAVVEVYDAATLKKRATLNLPAGAFAVRDMV
                     MSRDGKTCFVSHSVGRFTVPTTHLDRGWINTSAVSIFDVAACRYVNSVLLDDTVKGAA
                     NPWGLGVVGQDAWLCVALSGTHEMMVVDLKEMWKRLAAAANPAEIVNDLSFLYGAKTR
                     VSLEGQGAQGLAVCGERVFAAMRYADSLNLVEIWEDGPGAAVPLPLGPEPKPDILRLG
                     EMAFNDATLCYQHWLSCASCHPETRSDGTNWDLMNDGIGNPKQSRSLLYSHRASPVMI
                     TGIRASAEVAVAKGFELIQFHELPQDRLDSVDAYIKAQEPVPSPYLNPDGSMTAAALR
                     GKKIFEGKAACVKCHTPPYHSDKKKYTFGLGSDSERDRPFVTPILIEVWRTAPYMYDG
                     RAVTIQDVITTDNVNNKHGNTRDLTPDEVEDLAEYVNSL"
     gene            complement(11703..15626)
                     /locus_tag="PK576_03380"
     CDS             complement(11703..15626)
                     /locus_tag="PK576_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE60662.1"
                     /translation="MSHKGERIGVAFSVLLLAFAVRAEMTYYAHQGKISAVALVGEGK
                     SLGTGKVSKISSLGERKFGDSWNVEFSGELTVPAAGTYAFTLTSDDGSALFVAGKQVI
                     MNDGLHGPSKRTGKADLAAGKHQVSLVYFNFTGGHELKATVQPPNGPETDLAAACTPS
                     SVRHDFGELREQARQRLAEEARKAHERAEAAAQAALFEISSPEALVRSIRHLLKTKPR
                     DYKNGRAHLKRAEEFVRDLPALRERLKQGDEQAKNALLEFGKLKRQALVEDNPYLDFD
                     ETLVVVSDKVAVKANWVGTHTMAAKGYKNKLVRFNLRTGALTDVYAPTDGAYVGEPDL
                     HYDAQKLLFTSTDTNNMFQVMEVGLDGSNPRQVSTIVGEYVHNYGGIYLPDDKIVFSS
                     TAPMVGVPCIGGSTTVPNLYLMGPNGERPRQLTFEQDADWYPTVREDGQIMFLRWEYT
                     DIMHYYSRIMMTMNPDGSNQRAIYGTQSLWPNSMFYARPIPGKAGQFVAVVSGHHGVA
                     ASGKLTIFDTNKGHTHADGVVQHIPGYGKTVTHVTVDQLYPRVAPKLLKSFPELQKNV
                     DDLIAKHMPAESQQGKDYHELNNDFFNKCYPLLRGIYPEMALDLDTLVNGVYPQFLQP
                     YPLSANYFLTVAKLTGSAPWRLYLVDTFDNFVPLQPEKLDGYAYMVEPYPIRKRVRPP
                     VVPDRVNLQDKEAVCYVQNVYRGLGLKDVPVGTIDSLRIFTYAYGYYKIGSHDHIGVE
                     SGWDIKRLLGTVKVEPDGSAMFRIPANTTISLQPLDREGRAVQLFRSWLVAMPGEKLS
                     CIGCHEPPTEPPVTSKTIASGKPPQTIKPWRPRVDGFAFETEVQPVLDAYCVRCHDGS
                     NAKLPNFKGRAPDVPPRGYSEAYHAFHKYFRRPGPESASTMFIPYEYHMSTSEGAQLL
                     EKGHHGVKLDQESWLRLNAWVDLNVPYFGNWTSVYASNEKRHTWTTNISAYAASLRAQ
                     YANVHDDWESIPDQPYPVNVTRGKGEQRAAPVQVSAPQWPLTAEAAKALQQAQAGGNL
                     KRTIDLGNGLSITLVRIPAGEFVMGSDEETPWEQPRHRVRIARPFWISETEINNAAFF
                     TFKPDHNAGVFDQQWKDHVRLGYYSNYSEQPAVRMSWNDADAFCAWLGKKTGLKIALP
                     TEAQWEWACRAGSATPMSFGARDADFSPFANLADKSIEKFAVSGVNPVFRPNLVNNRV
                     FDFVPRVREIDDKQFLVSGTRQYQPNAWGVYDMHGNVAEWTRSDYVRYPYDAARSNSR
                     KPEVKKVVRGGSFYDRPYRATSSYRLGYVPWQGVFNVGFRVIIEE"
     gene            complement(15716..16417)
                     /locus_tag="PK576_03385"
     CDS             complement(15716..16417)
                     /locus_tag="PK576_03385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE60663.1"
                     /translation="MRRRLTRAVFFISCVAALTGMRVAGQDTAPACDSSGVCTLPDTA
                     VTVARPAGGRQFWAEKRLFTEAPPLEVARWLTDPPETKGKFLVIEFWRTWCGACKRGT
                     PFMNGLQKKYGKELAVIGITGEPEEVVRAYKGPKKEYFLALDRPKTETAESGGQNAEN
                     PPVEGTIPREKGQGAYEALFGVWGWPHVVILEPRFRTVVWEGYPGQKGYELTEALIEK
                     LLAIERAARAESGNP"
     gene            16604..17689
                     /locus_tag="PK576_03390"
     CDS             16604..17689
                     /locus_tag="PK576_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60664.1"
                     /translation="MTVRSSSQHSLRRGGRQLLALAGTTVIESIQQPAALLMLLTAVV
                     TTLLVPVFQFHRFGEDGRLARDSGLSCMLIFGMALAVSSAGHAVAGELTRGTAAAVLG
                     KPVARPLFLLAKWVGVLGVVTLFWCAQLAATLLAERISAHFLIRGDTAGYASDPITLL
                     LALTGLAVALFIAAARHAVLRRRFGVSAFLGIPISQGIVVLISGFYNRFGQLYPLYGE
                     AACEGCGSAHTHETAQVWLYHAELNLRVIPAALLLLFVLAVFAALAVALATRLPTGSV
                     LTVCAMTLLLGLAGDALFANAARFSARGLLAGVLPDLQHFWLCDRIARGGSVPGRYVA
                     EAGLYALTCCALFLTAGSVAFRNRDLG"
     gene            17738..18676
                     /locus_tag="PK576_03395"
     CDS             17738..18676
                     /locus_tag="PK576_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60665.1"
                     /translation="METRQVIETRGVRKTFRDFWRRPVVEAVKDLNLSVARGEVFGLL
                     GPNGSGKSTTLKMLLGLLRPTAGTIRVLDAPPQALQARARIGYLPEISHLHPFLTPRE
                     TLRYYASLFGLGRRETERRTEELLEMVDLTQAADRAVGRFSKGMARRVGLAQALMNAP
                     ELLILDEPTSGLDPLGARAVKDLVRTLAGAGVTVLMTSHLLADVEDVCDRVAILDCGA
                     LCAEGRTDDLLRQPDAVRFEIEGMDDAAAREARSALETRFGRPVRQSYPSLTLERYFL
                     QVVADGRTSDAFRLAPFLLGPQPDPEPSGGAPCATA"
     gene            18661..20058
                     /locus_tag="PK576_03400"
     CDS             18661..20058
                     /locus_tag="PK576_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60666.1"
                     /translation="MRNGLIPFLRQTGGTLRLAAHSMLRTRTIAAVLVLLAACVGLLP
                     RAVQGDGTPSGDIQILLQYTLGFAFFILCLATLWSACALLAAEIDSRRIHLTVVKPVR
                     PISLWLGRWLALLVIQAALLAVIYLGTYAQLHWRMRQDGWCPADRPASRRVTRPLLPS
                     PSEEARAILASMHANGTRPQGVSDAAILRTLETRAREKFDVVNPDQDVRWRFRLGRPL
                     QPGEPLTVRLRFDSEYSTREHVRGVCRIWPEAYPERVVEHRLNDFTLNEIALPVDTRA
                     FSDAAGHDPEGLRTFVLVFRHVGETRRVSALMLRFRQDVALLTAGGSFEANLARAALL
                     QWAVLALLAAFGLTLSACFSLPVAAFVATLTLVLTLLGHAVVRTVSVEEEQAWRNRVG
                     IWVSRGISSITSRAVQAEPLTSLTRGERIESDRIGETLIWNALLLPLGLAGMGVLMLR
                     HREWADTESCGKDHT"
     gene            20055..21470
                     /locus_tag="PK576_03405"
     CDS             20055..21470
                     /locus_tag="PK576_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60667.1"
                     /translation="MSFTRQRFLKTVLGAGAAFTGGCLGTRNAPPMRPARSAGRLRVA
                     LIGVGGQGLVNLRAVTDAADVAEPVALCDVDETVLLAARDRVTPQTSKVRLYKDFRVL
                     FDAERSLDAVFLSTPDHGHALQAAWALAAGCDLYIEPPAARTLGEVARMRMLARQQGA
                     LVQIGLRTATGEAFCRALEILDTGILGAVTVLHAWTNRPVWPQGNPRPEGSDIVPVGF
                     DWDLWLGGAPTRPYISRVYHPFNWRGWHDFGTGTLGDAGSDLLHLPFRALALQAPVTI
                     ESETAVPQTGESFPRASRLRFTFAARRRQPPVTLHWYDGGERPDAARLEPVTAAFGRL
                     PDAGCLLIGSRGLWFTSGDPTPRHFLALTGETSVREMTRHPACRDVPQCLPRTASPQR
                     EFLDALRNGTPTLSDLEHTSALTACILTGCVAQRIPGRLTWQARRNRFVNHVAANSLV
                     APVYREPWGEPENQIEERRTR"
BASE COUNT         3934 a         7308 c         6442 g         4159 t
ORIGIN      
        1 tgcccagata ggacgcggct tgaccggaca tgccgacaaa ctgctccgcc gaaatattgg
       61 ccgcgatcag cgagatgccg atcaaccacc aagtcagacc gcgccctgcc agaaagtaat
      121 ccgccgcgtc gctctcttga ctctgcacac tcttactctt ccagaggccg acactgagca
      181 ctgccgcgat gaaccccaca aaaatgacaa tatccaagat acccattgtt ctctccttcc
      241 tttcacttac ctgacaccga aacggtactc agtcgtcgac ttgtagatcg cgccggggcg
      301 cagaatcgtc gatgggaacg cgggctggtt gggcgagtcg ggcgagtgct gcgtctcaag
      361 cgccaagccg ccgcggtggc aaagattctt gcccggctgt ttgctgggca cctgatcggt
      421 caagaagttg ccgcagtaga actggattgc gggctcggtg gtccacacct ccatgtagcg
      481 tcccgtgctc ttctcataga gcgcggcggc tggcgcaagt tttccgctct ggtcgttcag
      541 cacccagttg tgatcgtagc caccaccgaa acgaagctgt tcgttgtcgg ctttaatacg
      601 cgcaccgatc agccagggcg tcgtgaaatc aaacggggtg cctttgaccg gcaccacctc
      661 gccggtgggg atcaggcctt tgttgaccgg tgtcatgcgg tccgccaaaa gcgtcaactc
      721 atgatccaga atcgtgccct cggcctcgcc cttgaaattg aaatagaggt gctgggtcag
      781 gttgaccggc gtcgccttat cggtcaccgc ctcatactcg atgcgccaca cattttccgg
      841 cgtgagccag taacgcacgg tcacatccag attgccggga tacccttggt cgccgtccgg
      901 actgcgcatc ttgaactgca cgcctatggt cttaccgtcc gaaagcggct cgctgtccca
      961 cacgtaggcg tcgaagcccc gtttgccacc gtgcagcgaa cagggcagcc cgcccggctc
     1021 gttgttgatc ggcagggtgt aggtcttgcc gtccagaacg aacttgccgt tcgcgatgcg
     1081 gttgccgtag cgcccgatca gcgacccaaa gtaagggtcg tgcgtctcgt agcccgcgac
     1141 atcattgaag cccagcgtga tatcagtctt ggcgcccgtg cgatccggcg taaacagacg
     1201 cacgaccttg ccgccgtaat cgatcacatc catcaccacg ccgcccgcac cggtcaaggt
     1261 gtactgctgc accaactggc cgtccttggt cttgccaaaa ggtttaacta ctaccgaagg
     1321 ctcgcaggta taccgcatat catctccgtc cttcttaaca ttccaaaacg aacggcagcc
     1381 gcataccata atgccgcatg ccgtaataca catcgccgca taacaagtgt ttcccatttt
     1441 cacactcccg atcttttgcc gacacttttc gtactccgcc cccatcggct ctgccgcgga
     1501 agcagcgcac ttccattatt gaaactcatt ggcatccaag taaggcttgc aaaaaacctc
     1561 atttacctga aaccacctat ggaatatcaa cggttgggga ttatataata tccgtctgca
     1621 gtcaccaagg tcaatttcag ggacaaaaga acccaattga ctgacacaga gaagtgtgta
     1681 aagctatgtg tggaggtgaa aaatggcaac gaatcttcaa ctcgatgacc gtttgatcgc
     1741 agaagcggca aggctgggaa aacaccgtac caagaaggag gctgtcacgc aggcgctgac
     1801 cgaatacgtt cggaatctgc ggcaggaagg gattttggat ctctttggca gcgtcgactt
     1861 cgatccgtct tacgattaca agcggcaacg gtcaaagtcg tgaaagtgct agtcgatacc
     1921 agcatctggt cgctagcctt gcgacgtcgc gaacacgcgc agaacccgga ggcggaagaa
     1981 cttggccgtc tgatcgcctc ccatgttgtg gagatcatag ggccgatccg gcaggagatt
     2041 ctgtctgggg ttcgggacgg tgcgcagttt gagcgtctag cgaggcatct tgatgctttt
     2101 gtggaccagc ctttgacggt cgaagattat gtgacggcag cgaagtttta caacctatgt
     2161 agatccaaag gaattcaggg ttccaacact gacttcctca tttgtgctgt tgcggtgcga
     2221 cacgattttt cgattttcac cacggacaaa gatttccttc actttgcaaa gtgcctgcca
     2281 atcgtgctac acgatgtcgg acaactaagg gacaggtaat ttggacgcaa aaaagcgtcg
     2341 gtattaaatt tagggggtgt cggtgttaaa atagatcacg ctaaggctat ggcgtctctt
     2401 agtcgcccac cgcgaacgcg gcaagcccct taccgcgccg caggtacagg acgccatccg
     2461 acaccaccgg cgaggaccaa tgcggcccgt tgccatactc gatcttgaac gaggagatca
     2521 cccgatggct gtcagccccg acctccacca acatcacggt tccgccgcgc tcgtcatacg
     2581 tgatcatgcc gacgggcgtg atgcaaaaag atgttttttt cgcgccctcc acatggtaca
     2641 ccgcgcggcc atccgccgga tcgaagcagg tcagccgccc tccgcccgag gagaagatgc
     2701 gccccttgta gaacgccacg ccctgaaagt ggttgtcaga ggatttgtcg gcccaaatct
     2761 gcttgaccgt accccccgcg atctcaagga tcgccgagcc gaggacgtat cccgaagaga
     2821 tgaaaacgcg atccttgcca cacaacacag gggtattggg gtgaaccgca tatttgttac
     2881 ggtgcgggta acgccacagc ggctgaccgg tcgcagcatc cacgcccacc acatggtctt
     2941 cgagcatggt gaccacctga cgccgcccgt tgatcgtgag cagggcaggc gaacagtagg
     3001 cgctacgctc gctcagcccc ttgctcttcc ataccgcccg accggttttg atgtccagcg
     3061 ccacgatcgc ggcatccggg ccacccggct gacagatcac cttaccgtca taaatcaacg
     3121 ggttgtcggc aatgccccac gtgatgttgc gtccgctgaa ctccgcatgg ctgtcgacac
     3181 gccacacgat atcgcctttt ttcgcatcga tgcacaccac atgcccgagg ccgccaacca
     3241 agtagagacg tccttcgtga aacgtcggcg aggtacgcgc gaactcatac gaagcacccc
     3301 actccggtcc gtactccacc tgccagcgga tcgcgccgct tttcgggtca agcgcaaaca
     3361 ggtgccccac gcggcttttc ttctcgccgg agctaccggt gatatagagc gtgccgccga
     3421 ccacaatcgg ttcggagtac ccttctccgg gcaactccac atgccacaac cgctgcggac
     3481 cgccgacagg ccactcgggc aacagccgct cctccgtgac gatcaagtcg cgattgctgc
     3541 cgcgccattg ggaccaatcc cccgaacggc ccgaaccggc caagcatgcc acagccaaca
     3601 tcaccgcaag ctgtcgtgtc atatgcatcc tttctgccat tccgccgcac tctccgaact
     3661 ttaccggaat tacgcgtaag tgtattgaat ccgcccatca ataccaagcc gaatttcgtt
     3721 gttgtcgccc ttctgcggtt cgcttacact gcgaatatga cagtagcccg gaatatcgtc
     3781 tggcatgttg cggcatgcgt gttcgcgctc ggtgcccggg tccaggccgc ggatcccggg
     3841 gaggcgtgcg ccacgcagac gctgacgttc gccctccccc gagtctgcat cacggcggac
     3901 ggccgcacgc gcgtcgggct cgcggggtgt gaatcggatg ccgtcaccgg cctgcccgtg
     3961 ctgccggtgg cgggagtgac gttttcggtt ccgccgggca tggcagttga tcgggttcag
     4021 gtcctccacg acgcggtcac ggaaattcct ctcgacgcgc ccgtggaatg ggggcagccg
     4081 ccgccccgca tcggagagat gccgtttccg gaggccccgc ccgatccggc cgtctacggc
     4141 ggcacgcacc cgtatcccga ccccgagcaa accgtctggc gcgtggaccc gaacggtgcc
     4201 gacgccgcgc ggctttccgt ccgagtctgc cccgtgcgct acgatccgca acgcagtctg
     4261 ctgctcgccg cggcctccgt caccgtcaca gtagagttcc gcgccctccg cccgaccggt
     4321 atcgcggtgc gaagcgcggc cactgccacc tccgaactga atgagatgcc cggaccgttc
     4381 acgtatgtga tcgtctccac ctccaacctg atccacaaca ccccggcgcc ctggaatttc
     4441 gcggcactgt gcgcggcgcg cgcccgcacc ggattcacca caacctgcgt cagcgcggaa
     4501 tggatcgact cccggtatgc gggtgaaaac cgggccgacc gcatccgcgc ctttgtccaa
     4561 gacgcgcacc ggaactgggg cacgcggttt ctgctgatcg gcggcacgtt cgacctcatc
     4621 ccggtccgta aactccgggt cgcggtcaac gagatcatcg tgacgcgcat cgccgacatc
     4681 ccggccgatg ccttatacta cggttgtctc gacgggccgt ttgacgggaa cggcaacggc
     4741 ctgttcggcg aacttcagga cggtgccaac ggcggggata tcgatttgct ggctgaggta
     4801 tatgtcggcc gctttcccgt ggccaatccc gaagaactct cccgcatgat ccgcaaaacg
     4861 ctgcggtatg aggccgcttc agcggaagaa ctcgcaccct gcgcattcct cgccgaaaag
     4921 atggatctcg ggctgatgac ttacaccggc ggcttcatgg aagaattgcg tttcggctcc
     4981 acgtacgacg ggcttaccac aatcgggttc gagacctcgg cattcgcgcc ggcgctggac
     5041 accgggcaca cgctctacga ctcggatgcc ggactctggt ccacggagca tgcgttcgct
     5101 ttcctgaacc ggaatttcgg gattctcaac catatcggac acgccgtcgc gaaacaatgc
     5161 atgaagatca acctgtacac ctccgcgcat caggccgcac tgcaggcctg caccaacact
     5221 ctgccgtatt tcgtgtattc gcagagctgt ctggccggcg cgttcgacac gccggattgc
     5281 ttcgcggaac aactggtcac cgtcagcaat gccgcgttcg ccgcagtcat gaacgcacgg
     5341 gaagggtggg tctacaacca tgttgtcggc gggttttcgc accgcttcca ccgctccttc
     5401 tgggatgcgg cgatgcgtgg caacggtacg cgcctcggcg agatcggcga atgcgcgcgc
     5461 cgcatgaacc tgcacttggt ctccccgagt tacggcacct actggcgttg ggttttttat
     5521 gaactcaccc tgttcggcga tccggccacg cccttcgcgg cccgggtcaa tcccacgccg
     5581 ccggttttcg atcatccggg gctgatcaac acctatgaga cccagaccgc ccaccgtgtc
     5641 gcctgttccc tcgaaccgat aggcctctac gatcccgctt ccgtgcgttt actctggcgt
     5701 accggttccg atccggagac ggtccacaca caatttctcg caaacgtttc cggcaaccgg
     5761 tacgaaacca cgatcccggc ccaccctgcg ggcacggcca tccgttacgc gctcttcgcg
     5821 gaaacgctcg ccggcgtcac cggccgctgg cctttggagg agccggagca cctcttccac
     5881 atcaccgacc gccttgacct cgagatccgc ggcacgcctg cggcgatcgg cacgccggat
     5941 cccggatacg gcaccactca ctatgcgtcg gggctggtgg ctgtcgcctc cgcgcccgaa
     6001 catgtggcgg tgaccgaaga gacgcgtcac acctgcatcg ggttcaccgg cggcggcagc
     6061 acgccgcctg ctggcgatca acccgatgtc gcattccgta tcgacacctc ctcgcacctg
     6121 acctggatct ggcagcgcga acacctgttt tccgtgacca gcgcgttatc cgagtttccg
     6181 cacacggcct tctgggtgcc ggagaacgag gcgtcgcccg taccacccgc gccggccacg
     6241 cttttcgtcg acggcgtgct ttacgccttt gccgaatggt ggtgcaacga tcaacgcctg
     6301 cccccggccc cggcccacag tccccatgac ctcggcgcga tcatcgcgga ccaaccccgc
     6361 cggttcaccg cacgttatct gccgatagag caggatacgg acggcaacgg gatcccggac
     6421 tggtgggaga cgcactatta cggccactcc ggtcgtctgc cggattcgga cgacgatcag
     6481 gatggctaca cgctggccga ggaatacacg gaccgtaccg accccctgcg cgcgacgagc
     6541 gtgcccgcac cgccggtcat cacgcatgag ccgcttgcgg cccggcagac gcatcccgcg
     6601 cccttcatcg tgcaggcgcg catcaccgac acccattgcg taacctccgc agtcctctac
     6661 tggtgccaca acacgaacct gtggcaaacc gtccccatga ccgccggttc caacgatctg
     6721 ttcttcgccg agatccacgc ggaatgcgcg cccggtgaca cgttcgaata ccggattgcc
     6781 gccaccgatc cgaccggacg caccgccgaa tcgggaccgt accccttcgc cctgttctat
     6841 ccgctgatcg acaccgtgca actccacgat ctcatgatcg tggcccaacc gacccagacg
     6901 cccttcaccg tgcgcatgga tctcgcaaac cggggcaacg ccgaactcta ctggcgcgtg
     6961 cagcccgcac ggattgaacg catcctctcc gagcgtttgc cctcctggga tttcacctca
     7021 ctcggccaac cctggagcgt ctccaccaac cgcgcgttct cgccccccta cgcgctccac
     7081 agccgtctgc tttccaacgc acaaccccgg accccggtac gcgcgagcct caccttcccc
     7141 gcagtgactc tggagcccgg cgccacgctc tcgttccggt actggatctt ctccgaggtt
     7201 caccaaaaca ccacccgggc gttcgacggc ggtctggtcg aaatctccac ggacggcggc
     7261 gagaccttca tgctgctgcg cgggccctat acccacacga tttacggctg gaacgcttct
     7321 ccctggcccg agggcacgcc ctgtttcgcg ggcaaaggga cggaaggatg gcagaccgca
     7381 gtttttgacc tggctgcgca ccatcccgag gcgaacggtt tcgcgggcag caacgtggtg
     7441 ttccggttcc actacggcgg cgacaacaac accgacaacg agggctggta tcttgacgat
     7501 atcgccgtgt ccccgatccg ctggccagcg ggcttcaact tttacggtgc cgccccggag
     7561 acccgcactc tggcgcccgg cgcacactac cgcctgcttt ggtccaccga cccatcggcc
     7621 atgaccatgc gcgacaacgc cgttgatatc ctttttgatt ccaacgagcc ggttcatcag
     7681 gatttcagcg tacgctggca ggtgatcctc cgcgaatttc cgaacctgcc cggattcaac
     7741 gccgtgcaga cttcgtccgg cgacggccgc gtacttttgg aaaccggtct cgcggaccgt
     7801 gacggcgagg cggtcagtct cgaggtcgcg tggtccccgg ataatggggg gcactggcaa
     7861 cccgccgcgc tgcatgatgt gaccgtatct gaaggcgatg ccgccgatga cgcgccggac
     7921 ggctgtcttg ccgcgttgcc gacctcccgc gcgggacaac cggtgaccaa cctcttccgc
     7981 gccttctggt cttcgcggga agcggtgccg cctgtcggcg tcaacaccca aacccggttc
     8041 cgcatcgtgg ctgacaacgg gttctttcaa gtgaccgccc tgacccagcg tgtgacggtg
     8101 gacaacctgc cgccccggtt cctgccgggc atgctcagtg ccgcgccgac aagtccgcag
     8161 gggggctacg cggtgaccga gaatctcctg acgctgcact ggcccgcagc cgaagacgag
     8221 cctgcggatt ccctcctccg gtaccgtctc acctcccagt gcggcacctc gattctggaa
     8281 cagacctccc acacgctcgc gctctcgaac gcgctcgacc agtcgcatgc gttccaagtg
     8341 gtcgcgttcg atccggcagg caacaccagc gttcctcttg acgccaccct cctcgtgctg
     8401 aatgcgttca gcgattatga cggcgacggt gtaaccacgg cggacgagga actggcggga
     8461 acccgtgccg acgacccgtc cgaccgtttc accgtccggt tgcgcgagtc cgccgatgca
     8521 ctcacgctct cctggcccgg cgttgtcggg cggctctaca caatcgaaac cactccctcg
     8581 ctagcccccg ccgtttggca accgctggcc gggcacattg acatccccgg caccggcgcg
     8641 atgcacgacg ccgttttgcc gcgcaccacc ccaacttcgt tttaccgaat ccggatcaca
     8701 ttgccatgac gacgagtttt cccttccctt tccgtcccgt tgatgccccg tatttccgga
     8761 ttgccgtcct gtgcgccgtg ctggctggcg gctgcagcag gcagcctgcc gcgccccttc
     8821 gcatcgaatg gctctgcatg gggacggtcg ccgcagtgca gtgtgccgac cggagccgcg
     8881 caacggacgc gcgcgatcag gcgcaggcgg tgttcgccga acttgatcgt gaattctccg
     8941 cctggcacgg ggaaagcgtg ctctcggcgg tcaaccgcgc agccggcagc ggccaagcga
     9001 ccgccgtctc cgcttgtttc gcgactgtgc tgcaaaccgc gctggacgcc tcggaagcca
     9061 gtggcggcgc gttcaacccg atgatcggac cggtgatgca ggcctgggga ttcaacggcg
     9121 cgatgcagcc cccggagtat ccggacgacc gcatactgaa cgcggccttt gcgatggcgg
     9181 actggcgcgc cgtgatctgc aacaaaacga ccgacggggc agaggttcgt ttgcccgtgg
     9241 cgggcatgtc gctggacctc ggggccatcg ccaagggcta cgcggtggat ttggcatggg
     9301 agcgtctcaa agccgcaggg cacaccaatc tgctgatcga tctcgggggc aatctgcgcg
     9361 cacttggcga ggctgcgccg gggcgcggcg gctggcgcac cggcgtgcgc aatccctttc
     9421 agtccaacac gatggtcggc aagtttctca tgcgccacgg cgaggcggtc gccacctcag
     9481 gcaattacga gcggtttgtc gaaatccaag gcctccgcta cgcccacatt ctggatggcc
     9541 gcaccggcct gccggtcacc ggcatggcga gcgtcacggt gatcgcgccg gacgccatga
     9601 ccgcagacct gctctccacc accctcttta tcctcggtcc ggaagcgggg gtacggctgc
     9661 tgagccggta tcccggctgt gaggcactct ggattcccga cacgccagac cagctcaccc
     9721 tgttgagcac ccccggattt gccgaccggc tcacgccgca cggcaagaca ccgtacgcgc
     9781 tctccgtaat ccggtaaggc gaccgtcgtt tgacgcggcg cgcggatccg acccggtgtc
     9841 agagcgagtt cacatactcc gccaggtcct cgacttcatc cggcgtcagg tcccgggtgt
     9901 tcccgtgttt gttgttgacg ttatcggttg tgatcacgtc ctgaatcgtc accgcgcggc
     9961 cgtcgtacat gtagggcgcg gtccgccaca cctcgatgag gatcggcgtc acaaagggac
    10021 ggtcacgctc actgtcggag ccgagtccga acgtatactt cttcttgtcg ctgtgataag
    10081 gcggcgtgtg gcacttcacg caggcggcct taccctcaaa gattttcttg ccgcgcaacg
    10141 ccgcggcggt catggatcca tcgggattga gatacggact cggcaccggc tcctgcgctt
    10201 tgatgtaggc gtccaccgag tccagcctgt cttgcggcaa ctcgtggaac tggatgagct
    10261 cgaacccctt ggccaccgcc acctcggcgg aggcgcgtat gcccgtaatc atcaccggcg
    10321 aagcccggtg cgaatagagc agactgcgcg actgcttcgg gttgccgatc ccgtcattca
    10381 tcaaatccca gttggtgcca tccgaacgtg tttccggatg acaactggcg caactcagcc
    10441 aatgctggta gcagagcgtc gcgtcgttaa acgccatctc accgagccgc aagatgtccg
    10501 gcttgggttc cggaccgagc ggcaacggaa cagcggcccc tggaccgtcc tcccaaatct
    10561 ccaccagatt caggctgtcg gcataccgca tggcggcaaa gacccgctcg ccgcagacgg
    10621 ccaatccctg cgcaccctga ccctccagag acacgcgcgt tttagcgccg tagagaaaac
    10681 tgaggtcatt gacgatctcg gccgggttcg cggcagcggc caaacgcttc cacatctctt
    10741 tgagatcgac aaccatcatc tcatgcgtgc cggaaagggc cacgcacagc caagcgtcct
    10801 gaccgaccac accaagcccc cacgggttcg cggcgccctt caccgtatcg tccagcaaca
    10861 cggaattcac ataccggcat gccgcgacat cgaagatcga cacggcgctg gtattgatcc
    10921 atccgcggtc gagatgcgtg gtgggcaccg tgaagcgtcc gaccgaatgc gagacgaagc
    10981 aggttttgcc gtcccggctc atcaccatat cccggacggc gaaggcgccg gcgggcaggt
    11041 tcaacgtcgc acgctttttc agggtcgcgg catcatacac ttccaccacg gcgtaaacgt
    11101 gctgcgcgtt ggcaggcatc agcggcaaat agttgcccac ccacagcgtg gaaccgtcat
    11161 ccgacaggca gcttgcgcat ggctcgcgca cagctttggc ggtgttccgg accttccacg
    11221 aagcgagatc cacggcatgc acattcggtt ggtcggcccg cgcgaaccgg ttgcagtaat
    11281 agagcgtttt gccatccgca ctgaggcaca gcgcctcggg cgtgtggcct gcaggaagcg
    11341 tccggaggat ccgtccgctt ttccggtcca actcatgcag cagaccccgg gcaccacccg
    11401 cggtggcata gatccggtcc tgctcgtccg cgacagccag ccgggcgacc ccttcgaccg
    11461 cataacgggc ggtgaccttt tccgattcga catcgaacac cagcaccttg ccggcggtct
    11521 cacatgccac atacagggtt ttaccggccc ggtccgccac cacatccaca ggcgtcaaag
    11581 gcgtttccgc agccgttgcc tgcacgtata ccagcgcaac cgccaccccg atccaccgcg
    11641 cggcaatgcg cgccatcttt ccatctatcc ccatcccccg catgatctct ccccctgccg
    11701 catcactcct cgatgatgac gcggaacccg acattgaaca ccccctgcca cggcacatat
    11761 cccaaccggt agctggatgt cgcacgatag gggcggtcat agaacgagcc accgcgcacg
    11821 actttcttca cctccggttt ccgggagttg gaccgggccg cgtcgtacgg ataccgcaca
    11881 tagtcgctgc gcgtccactc tgccacgttg ccgtgcatgt catacacgcc ccacgcgttc
    11941 ggctggtact gccgggtgcc ggagaccaga aactgcttgt cgtcgatctc cctcacacgc
    12001 ggcacaaagt cgaacacgcg gttgttgacc agattgggac gaaacaccgg gttcacacca
    12061 ctcaccgcga acttttcgat cgacttgtcc gcgaggttgg cgaacgggct gaagtccgca
    12121 tcccgcgcgc cgaaggacat cggggttgcg ctgcccgcgc ggcaggccca ctcccattgc
    12181 gcctcggtcg gcagggcgat cttcaggccg gtctttttgc cgagccacgc gcagaacgca
    12241 tcggcgtcgt tccaactcat acgcactgcg ggctgttccg aataattcga gtagtagccg
    12301 agtcgcacat ggtccttcca ctgctggtcg aacacaccgg cgttgtggtc cggcttgaag
    12361 gtgaaaaaag ccgcgttgtt gatttccgtt tcggaaatcc agaaaggccg cgcgatccgg
    12421 acgcggtgac gcggctgctc ccaaggcgtt tcctcatcgc tgcccatcac gaactcgcca
    12481 gccgggatgc ggaccagcgt gatgctgagc ccgttcccga ggtcgatcgt ccgtttcagg
    12541 ttaccgcccg cctgcgcctg ttgaagcgct ttcgcggcct cggcggtcaa gggccactgc
    12601 ggcgccgaaa cctgaaccgg cgcggcacgc tgctccccct tcccgcgggt cacgttgacc
    12661 ggatacggct ggtcgggaat ggactcccaa tcgtcgtgca cgttcgcgta ctgcgcccgc
    12721 agcgacgccg catacgcgga gatgttggtg gtccaagtat ggcgtttctc attggaggcg
    12781 tagaccgagg tccagtttcc gaaatacggc acattcagat ccacccatgc gttgagccgc
    12841 aaccaggact cctgatcgag tttcacaccg tgatgcccct tttcaagcag ttgcgcgccc
    12901 tcggaggtgg acatgtgata ctcatacggg atgaacatcg tgctggcact ctcgggaccg
    12961 ggacggcgga aatacttgtg gaacgcatga tatgcttcac tataaccgcg gggaggtaca
    13021 tccggagcac gtcccttgaa attcgggagt ttggcgttgc tgccatcatg gcaacgcacg
    13081 caataggcat ccagcaccgg ctggacctcg gtctcgaacg cgaagccatc cacgcgcgga
    13141 cgccagggtt tgatcgtctg cggcggcttg ccggaggcga tcgtcttgct ggtcaccggc
    13201 ggctcggtcg gcggctcatg acacccgatg cacgaaagct tttcaccggg catggccacc
    13261 agccaactgc ggaagagctg aaccgcccgg ccctcccgat ccagaggctg gagcgagatc
    13321 gtcgtgttgg ccggaatgcg gaacatggcc gacccgtccg gctcgacctt gacggtcccc
    13381 agcaggcgct tgatatccca gccgctctcc acaccgatgt ggtcgtggct gccgatcttg
    13441 taatatccgt aggcatatgt gaagatgcgc agcgagtcaa tcgttccgac cggcacatcc
    13501 ttgagtccga ggccgcggta gacgttctgg acataacaga ccgcctcctt gtcttgcaga
    13561 ttcacgcggt cagggaccac cggcggacga acgcgcttgc ggatcggata gggctcgacc
    13621 atatatgcgt agccatccag tttctcgggc tgtagcggca cgaagttgtc gaaggtgtcc
    13681 accagataga gccgccacgg tgccgaaccg gtgagtttgg cgacggtcag gaagtagttc
    13741 gcgctgagcg gatacggctg gaggaactgc ggatacaccc cgttcaccag cgtgtcgaga
    13801 tccagcgcca tctcgggata gatgccgcgc aacagcggat agcacttgtt gaagaagtca
    13861 ttgttcaact cgtgataatc cttgccttgc tgcgattccg caggcatgtg tttcgcgatc
    13921 aggtcatcca cgttcttctg caattccggg aagctcttca gcagcttcgg cgcgacgcgg
    13981 ggataaagct ggtccacagt gacatgggtc acggtcttgc cgtatccggg gatgtgctgc
    14041 accacaccgt cggcgtgggt gtgccccttg ttggtgtcga agatcgtcag cttgcccgac
    14101 gcggcgacgc cgtggtgccc ggagaccacg gccacgaact ggcccgcctt gccggggatc
    14161 ggacgcgcat agaacatgga gttgggccac aggctctgcg tcccgtaaat cgcccgctgg
    14221 ttgctgccgt ccggattcat ggtcatcatg atgcgggagt agtagtgcat gatgtccgta
    14281 tactcccagc gcaaaaacat gatctgcccg tcctcgcgca cggtcggata ccagtccgca
    14341 tcctgttcaa aggtcagttg gcgcggccgc tcgccgttgg ggcccatgag gtacaggttc
    14401 ggcacggtcg ttgaaccgcc gatacagggc acgccgacca tcggcgcggt cgaactgaaa
    14461 acgatcttgt cgtccggcag atagatcccg ccgtagttgt gcacatactc cccgacgatg
    14521 gtcgagacct ggcgggggtt gcttccgtcc agccccacct ccatcacctg aaacatgttg
    14581 ttggtgtccg ttgaggtgaa aagcagtttt tgcgcgtcat agtgcaggtc cggctcgccg
    14641 acatacgcgc cgtccgtggg cgcgtacaca tcggtcaggg cgccggtgcg caggttgaac
    14701 cggaccagct tgttcttgta gcccttggcc gccatggtgt gcgtccccac ccagttggcc
    14761 ttgaccgcca ccttgtcgga caccaccacc agcgtctcgt cgaaatcaag atacgggttg
    14821 tcctccacca gtgcctgacg cttgagcttg ccgaactcca gcagggcgtt tttcgcctgt
    14881 tcatcgccct gcttgagacg ctcccgcaac gcgggcaggt cccgcacgaa ctcctcggcc
    14941 cgcttgagat gggcacggcc gttcttgtag tcgcgcggtt ttgttttcag gagatggcgg
    15001 atggaacgca ccagcgcctc gggcgatgag atctcgaaca gcgccgcctg tgccgcggcc
    15061 tccgcgcgct catgggcctt gcgcgcctct tccgccaagc gctgacgggc ctgctcccgc
    15121 aactcgccga aatcgtggcg gacggaactc ggcgtgcagg ctgccgccag atccgtctcc
    15181 gggccgttgg ggggctgcac cgtggctttc aactcatgtc cgccggtgaa gttgaaatag
    15241 accagcgaga cctggtgctt gccggccgcg agatccgctt tgccggtccg cttcgagggt
    15301 ccgtgcaacc cgtcgttcat gatcacctgc ttaccggcca caaacagcgc gctgccgtca
    15361 tccgaggtca gggtaaaggc ataggtcccc gctgcaggca cagtcaattc gccggagaac
    15421 tcgacgttcc acgagtcgcc gaacttacgc tcgcccaaag aactgatctt cgaaaccttg
    15481 ccggtgccga gcgattttcc ctcccccacc agcgcgacgg cagagatttt cccctgatgt
    15541 gcgtaatacg tcatttcggc ccggacggca aacgccaaca acaagaccga aaacgcgact
    15601 ccgatccgtt cccccttatg cgacatatcc cctccactga attccgcccc gcagggcttg
    15661 aaaataaatt gatcttaatc catccggcct gtttgggcaa gcttcgaaac aggactcacg
    15721 gattgccgga ttccgcccgc gccgcacgct cgatcgcgag caacttctca atcagggcct
    15781 cggtcagctc gtaacctttc tgcccgggat acccctccca caccaccgta cgaaaacggg
    15841 gttcgaggat cacgacatgc ggccaccccc acacaccgaa gagggcctca tacgcgccct
    15901 gccccttctc gcgcggaatc gtcccctcga caggcgggtt ttcggcattc tggcccccgc
    15961 tctccgcagt ctcggtcttc ggacggtcca gcgccaagaa atactctttc ttcggccctt
    16021 tgtaggcgcg gacaacttct tcaggctcgc cggtgatgcc gatcacggcc agctccttac
    16081 cgtatttctt ctgcagcccg ttcatgaacg gcgtgccgcg tttgcacgcg ccgcaccagg
    16141 tccgccagaa ctcgatcacc agaaacttgc ctttggtctc gggcgggtcc gtaagccagc
    16201 gcgccacctc cagcggcggc gcctcggtga acaaccgttt ctccgcccag aactgccgac
    16261 ccccggccgg ccgggcgacc gtaaccgccg tgtccggaag cgtgcagacc cccgagctgt
    16321 cgcacgctgg cgcggtatcc tgccccgcga cgcgcatacc cgtcagcgca gcgacgcaac
    16381 tgataaaaaa tacggcacga gtcaaacggc gtctcatctc cggtcttcct ttccctgact
    16441 actccgcccg tacccccaac gacacggacg cctgcccaag catggctggt ttcccaaaac
    16501 aatgcggggt gtagataaag gacaggataa cacatttcgg attgcgccgt acacccctta
    16561 tccgactttg acgcgcggga cagcttcggg taaactgatt tttatgaccg tacgttcctc
    16621 ctctcagcac agcttgcggc gcggcggccg acaactgttg gcgctggcag ggaccactgt
    16681 gatcgagtcg atccagcagc cggccgcgtt gctgatgctc ctgaccgcag tggtgaccac
    16741 actgctcgtg ccggtcttcc agttccaccg gttcggcgag gacggccggc tggcgcggga
    16801 cagcggcctc tcctgcatgc tgatcttcgg gatggcactc gccgtaagca gcgccgggca
    16861 cgcggtcgcg ggcgagctga cacgcggcac tgcggcggcc gtgctgggta agccggtggc
    16921 gcgtcccctt ttcctcttgg ccaaatgggt gggcgtgctc ggcgtggtga cgctcttctg
    16981 gtgcgcccaa ctggcggcca cgctgctcgc cgagcgcatt tccgcccatt ttctgattcg
    17041 gggcgacacc gcaggatacg cctcggaccc gatcacgctg ctactggcgc tcaccgggct
    17101 cgcggtggcg ctcttcatcg ccgcggcgcg ccacgccgtg ctgcgccgcc ggttcggcgt
    17161 gtcagccttt ctcggcatac cgatttcgca ggggatcgtc gtgctgatct ccggtttcta
    17221 caaccggttc ggtcaactct atccgttata cggcgaagcg gcctgtgaag gctgcggcag
    17281 cgcgcacacc catgaaaccg cgcaggtttg gctctaccac gcggaactca atctgcgcgt
    17341 gatccccgca gccctgctcc tgctcttcgt tctcgccgta ttcgccgcgc tggccgtggc
    17401 tctggcgaca cgcctgccga ccggttccgt attgacggtc tgcgccatga ccctgctgct
    17461 cggcctggcg ggtgacgcgc tgttcgcgaa cgcggcgcgg ttctccgcac gcggcctgct
    17521 ggcaggcgtg ctgcctgatc ttcagcattt ctggctttgt gaccggatcg cgcgcggtgg
    17581 cagcgtgccg ggacgttatg tggccgaagc cggcctctac gcgctgacct gctgtgccct
    17641 gtttctgacg gcgggcagtg tggcgttccg caaccgggat ttgggatgaa ccgcaggacg
    17701 cgcggcgcgc gggggtctgc gaaacggagt gaggcacatg gagacgcgac aagtcatcga
    17761 aacgcgcggc gtgcgcaaaa cgttccgcga cttctggcgg cggccggtgg tcgaggcggt
    17821 gaaagacctg aacctctcgg ttgcgcgggg cgaggtcttc gggctactgg gcccgaacgg
    17881 atccggcaaa agcacaaccc ttaaaatgct gctgggtctg ttgcgcccca ccgccggcac
    17941 gatccgtgtt ttggacgcgc cgccccaggc gctgcaggcc cgggcgcgga tcggatacct
    18001 gccggaaatt tcgcacctgc atccgtttct gaccccacgc gaaacgctgc gctattacgc
    18061 cagcctgttc ggactcggac gccgcgagac cgagcggcgc acggaagagt tgctggagat
    18121 ggtggatctg acgcaagcgg ccgaccgggc cgtgggccgc ttctccaagg gaatggcacg
    18181 ccgcgtgggg ttggcccagg cgctgatgaa cgcgccggaa ttgctgattc tggacgaacc
    18241 gacctccgga ctcgatccgc tcggcgcacg cgcggtcaag gatctcgtgc gaactctggc
    18301 cggggcgggc gtgacggtct tgatgacctc ccatctgctg gcggatgtgg aggacgtctg
    18361 cgaccgtgtg gcgattctcg actgcggcgc gctgtgcgcg gagggccgta ccgacgacct
    18421 gctgcgccaa ccggatgcgg tgcggttcga gatcgaaggg atggatgacg ccgcggcccg
    18481 cgaagcgcgc agtgcgttgg agacgcgttt cggacgcccc gttcggcaaa gttacccctc
    18541 cctcacgctg gagcgctact tcctgcaggt ggtggcggat ggaagaacgt cggatgcctt
    18601 ccgtctggcc ccttttctgc tcggtccgca acctgacccc gaaccgtccg gaggcgcgcc
    18661 atgcgcaacg gcctgatccc gtttctccgg cagaccggag gcaccctgcg gctggccgcg
    18721 cactcgatgc tccgtacccg cacgatcgcc gcggtgctgg tgttgctggc ggcctgcgtg
    18781 ggattgttgc cccgtgccgt tcaaggtgac ggcacgccct ccggcgatat tcagattctg
    18841 ctccaataca cgctggggtt cgcctttttc atcctctgcc tcgccacact atggtcggcc
    18901 tgcgcgcttc ttgccgccga aatcgacagc cgccgcatcc acctcacagt cgtcaaaccg
    18961 gtgcggccca tcagcctctg gctcggcaga tggctggccc tgctcgtgat ccaagcagcg
    19021 ttgctggccg tgatctatct cggaacctac gcccaactcc actggcgcat gcgacaggac
    19081 ggctggtgcc ctgccgaccg gcccgccagc cgaagggtga cgcgaccctt gctgccgtcg
    19141 ccgagcgagg aggcgcgcgc gatactggcc tccatgcacg ccaacggaac ccgaccccaa
    19201 ggtgtgagcg atgccgcaat cctgcgcacg cttgaaaccc gggcccgcga aaaattcgat
    19261 gtggtgaacc ccgatcaaga tgtgcggtgg cgtttccggc tcggccgtcc gctccagccg
    19321 ggcgagcccc tgaccgtccg gttgcgtttc gattcggagt acagcacacg cgagcatgtc
    19381 cgcggcgtgt gccgcatctg gccggaggcg tatccggaac gggtcgtgga gcatcgcctc
    19441 aacgatttca ccctgaatga aatcgccctc cccgtggaca cccgcgcctt ttccgatgcg
    19501 gccggtcatg atcccgaagg cctgcgcacc ttcgtcctcg ttttccggca cgtcggcgag
    19561 acgcgccgcg tctcggccct gatgctgcgt ttccggcagg atgtggcatt gctgacggca
    19621 ggcggatcgt ttgaggccaa tctggcgcgc gccgccctgc tccagtgggc tgtgctggct
    19681 ttgctggcag ccttcggcct gaccctgagc gcctgtttct cgctgccggt agccgcattt
    19741 gtcgcgaccc tgacgctggt gctgaccctg ctgggccacg cggttgtgcg tacggtttcc
    19801 gttgaagagg aacaggcatg gcggaaccgg gtcggcatct gggtctcgcg cggcatcagt
    19861 tccatcacga gccgggcggt gcaggccgaa cccctgacct cactgacccg cggcgagcgt
    19921 atcgaatcgg accggatcgg tgaaaccctc atctggaatg cacttctgtt gcccttgggt
    19981 ctggcgggaa tgggagtgct gatgttgcgc caccgggaat gggcagacac ggagtcatgc
    20041 ggaaaggacc acacatgagt ttcacccgtc agaggtttct gaaaacggtt ttgggcgcag
    20101 gcgccgcatt cacgggcggc tgtctcggaa cgcggaacgc accgcccatg cggcctgcgc
    20161 gatccgccgg tcggctccgc gtcgccttga tcggagtcgg cggacaggga ctggtcaatc
    20221 tgcgggcggt aaccgatgcg gccgatgtgg ccgaacccgt ggcgctctgc gatgtggacg
    20281 agaccgttct gctggccgca cgtgaccgtg tgacgccaca gacgtccaaa gtgcgtctct
    20341 acaaagattt ccgggtattg ttcgatgccg aacgctcgct ggacgcggtc ttcctcagca
    20401 cgccggatca cggacacgcc ctgcaggccg cctgggcttt ggccgcaggt tgcgacctgt
    20461 acatcgaacc cccggccgca cggacccttg gcgaggtggc gcgcatgcgg atgttggcac
    20521 ggcaacaagg cgcgctcgtg cagatcggtt tgcggaccgc cacgggggag gcgttctgcc
    20581 gcgccctcga aattctggac accggcatcc tcggcgcggt caccgttctc catgcctgga
    20641 caaaccggcc ggtctggccg cagggtaatc cgcgcccgga gggcagtgac atcgtgcctg
    20701 ttggtttcga ttgggatctg tggctgggcg gcgcgccgac gcggccctac atatcacggg
    20761 tctaccatcc cttcaactgg cgcggctggc acgatttcgg aaccggcacg ttgggcgacg
    20821 caggcagtga tctgctgcac ctgccgttcc gcgcgctcgc gttgcaggcg cccgtgacca
    20881 tcgaatcgga aactgcggtg ccgcagaccg gagaaagttt cccgcgcgcc agtcggctcc
    20941 ggttcacctt tgcggcgcgc cgacgccaac cgccggtgac gctgcactgg tatgacggcg
    21001 gcgaacggcc cgatgcggca cggttggaac cggtcacggc ggcattcggt cgactgccgg
    21061 acgccggctg cctgctgatc ggatcgcgcg ggctctggtt cacctccggt gaccccacgc
    21121 cccgacattt tctcgcgctg accggcgaaa cgagcgtgcg ggagatgacc cgccatccgg
    21181 cctgccggga cgtgccgcaa tgcctgccgc gcaccgcgag tccgcagcgc gagttcctcg
    21241 acgcgctgcg gaacgggacg cccacgctct cggatctgga gcacacctcc gcgctgaccg
    21301 cctgcatcct gaccgggtgc gtggcgcaac ggatcccggg gcgcttgacg tggcaggcac
    21361 gacgcaaccg tttcgtgaat catgtggcgg caaacagcct ggtcgcccca gtctaccggg
    21421 aaccgtgggg cgaaccggaa aaccagattg aagagagaag gacaagatga cggttgatcg
    21481 ctacaccatg gggcgggaaa aggatcttct ggcggaacag gagcgtacgc tcgcgacgcc
    21541 gaagctgcct gcaaaaccgg ccgttccacc gcctacaaca acgacatccg tctcgtggaa
    21601 gacaggaagc cgatgggctg gttcgaaaac ctcgccgtct acaatccgac agctcggact
    21661 tcgaagcgac aggttcatcc ccccacaacc tttccagttg caagcccggc gactccgatc
    21721 cccatgcttc tcagaaaatc atgttttgaa atcataacca tctccttcac cttcgttttc
    21781 catgaacagc atcaccacgt ccgaaagacg ccgaaccagc ggcggctgtt tgattcgctg
    21841 ccg
//
LOCUS       DAOSLE010000027        21688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2675_length_21688_cov_7.257200, whole genome shotgun sequence.
ACCESSION   DAOSLE010000027 DAOSLE010000000
VERSION     DAOSLE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21688
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2675_length_21688_cov_7.257200"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK576_03410"
     CDS             <1..407
                     /locus_tag="PK576_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60668.1"
                     /translation="GLATNATTMLATFDGVLERMGVDGSESDAPPAQVAAKEEGTPFR
                     IQDYSEAAERLEASALRLTELLQTFDQTLGANSREALAAQLDPVVRRTRAETEALVDH
                     LFRRALQLTGALLAAALAYRLIASRISRTPRA"
     gene            complement(835..2133)
                     /gene="larA"
                     /locus_tag="PK576_03415"
     CDS             complement(835..2133)
                     /gene="larA"
                     /locus_tag="PK576_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOE60669.1"
                     /translation="MPTIRIPYHRGHLEATLPANVRLTLLEPTGTACTVSPSEAEQRQ
                     RVCAALDHPVGTPRLETLAAGKASAVLITSDHTRPVPSRITLPLLLERLRRGNPGIAI
                     QILVATGCHRPTSDAELREKFGAELVARETFVMHDCTDTANLRMLDQLPSGGALWLNR
                     LALETELLVAEGFIEPHFFAGFSGGRKSVLPGIAGRETVLANHCAAFIADPHARAGQL
                     ERNPIHRDMLFAARQARLAFILNVTLHPDKTIRTAVAGHAEAAHEQGCRDMRRAVSVP
                     RAEAEIVITGNGGYPLDQNLYQAVKGMTAAEACCVPGGTIIMVAGCCDGSGGESFYRA
                     LAGARSPAELLAEVLRVPQALTPPDQWEYQILARILSRHRVILVTDLWDADAIRALHI
                     TPAASLDEALALAWHEHGTSARITVIPDGVSVIVEPKKSS"
     gene            2478..3743
                     /gene="icd"
                     /locus_tag="PK576_03420"
     CDS             2478..3743
                     /gene="icd"
                     /locus_tag="PK576_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE60670.1"
                     /translation="MAYQHIQIPSFGEPITRGDDGRLHVPDCPVIPFIEGDGTGPDIT
                     RAARIAWDAAVQTAYGEKRKIAWMEIYAGEKANAVYGPGTWLPDETLEACKAYLVSIK
                     GPLTTPVGGGIRSLNVALRQELDLYVCLRPVRWFQGVPSPVKRPELTDMVIFRENTED
                     IYAGIEWLAESDEARKVIAFLQNEMGVKKIRFPGSSSIGIKPVSIEGTRRLVKAALDY
                     AIDNDRSSVTLVHKGNIMKFTEGGFRDWGYQLAREAYGAALIGDGPWCRFKNPKTGRE
                     ITVKDCIADAFLQQILTRPAEYDVIATLNLNGDYISDALAACVGGIGIAPGGNINYTT
                     GVAVFEATHGTAPKYANLDKVNPGSLILSGEMMLRYMGWTEAADKLISAMDRVIANKT
                     VTYDFARLMEGAVEVSCSAFGRALADAMQ"
     gene            3896..4948
                     /locus_tag="PK576_03425"
     CDS             3896..4948
                     /locus_tag="PK576_03425"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE60671.1"
                     /translation="MSRSQYGAVLFLLFAGWIVAAGCGKQETTAADGSRPVKLTYSVF
                     FPPTHKQAKLAEAWAEEIGRRTQGKVRITVFCGGSLTKADQCYQGVVDGISDIGMSAF
                     AYTRGRFPLLEGLDLPVGYADGVSATRIVNELCHAFLPKEIRDVKLLYVHAHGPGILA
                     SKRPMRTLADLKGAKVRATGFCAKVVESLGGSPVSMPQGDTYEALQKGVVDATFCPVE
                     TLKGWKQGEVIRNITDSRCIGYTTSFFVVINPVRWASLPDEVKQIMEDVSKEWVERHA
                     QAWNEADDEGLAFARSLDREVVPLDPSEQVLWRKQVEPLLREYAERAEAKGLPGKDFL
                     NRLTTAVREAQEGSSR"
     gene            4945..5463
                     /locus_tag="PK576_03430"
     CDS             4945..5463
                     /locus_tag="PK576_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOE60672.1"
                     /translation="MSPAQKPVRKPLGPFWQSVVGLCRFMTGIACCGLAVMTALVCVD
                     VVLRLAGHPVKGVYDLVRIVAAVTVACSLPITTAKKGHVAIEYFFLKLNRVGRLVVDS
                     VMRLLMIAAFLLTATECVGYGLRFLRNRQVSDTIEVPIFWVPWVMAVAFAVTALVVVF
                     HLVYPGREMVKS"
     gene            5460..6761
                     /locus_tag="PK576_03435"
     CDS             5460..6761
                     /locus_tag="PK576_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE60673.1"
                     /translation="MTPVMFGVVGCVALFILLLCSMPVAIVMGLVGVVGYAAVVSGKA
                     ALAMLSADLYDVFSSYNLTVIPLFIFMGQVAFHTGISGRLFTAANSWLSGLKGGLAMA
                     TVGACAAFGAICGSGPATAATMAAVALPEMRKHKYDDALASGTVASAGGLGMLIPPSV
                     VFIVYGILTEQSIGKLFIAGILPGIVVALLFCLTIHVLCLANPALAPKAVRTPWRVRF
                     RSLSGVTETMILFVLVMGGMFAGWFTPTEGAAIGAAGSVAIAVCGGHGSWALFRQAAQ
                     ETIRTSCMVLVIVAGATMFGHFLAVTGLPTQFAAWLAALPLPSWVVMLLILFFYLLAG
                     CFVDSLALILLTIPIFYPVVMGLGFDPIWFGVMIVLVTQMGVITPPVGVNVYVVSGIA
                     RDIPLQTVFRGAIPFLFALIVACGILMLFPGLATWLPGVVR"
     gene            6783..7289
                     /locus_tag="PK576_03440"
     CDS             6783..7289
                     /locus_tag="PK576_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE60674.1"
                     /translation="MDGSTARHGNYRKILFCTDFSANADFAFHFAVDAALRNTGSTLY
                     LLHVIPEPDAQFWKGYIYEVGDMDAKARADIDRMIDTVYRPRVPSDVTFQIAMRIGDA
                     ARMILDFAASQDVDLIVLGRQGRGAITQWLLGNVTGKVVRRSTCPVLVVPQAFKTRQS
                     EPAAADEQ"
     gene            complement(7314..7604)
                     /locus_tag="PK576_03445"
     CDS             complement(7314..7604)
                     /locus_tag="PK576_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOE60675.1"
                     /translation="MSWVVETRDGCVVTVKATPRANRSEIAGVDADWLRVRLQAPPVD
                     GKANAALIAFLAKVAGVPKRSVVLLSGETSRLKRVALCGVTAAAARQAFAKQ"
     gene            complement(7612..8517)
                     /locus_tag="PK576_03450"
     CDS             complement(7612..8517)
                     /locus_tag="PK576_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60676.1"
                     /translation="MFYLFLLFHFVFLLFWLRFWSAPEREFYFNPFVSGTINLTDRVI
                     LFLRPVLAIPEAMTALVILIVVVLLQTLLAARLQFPLGVTLGTTFHFTPPAVSDNPLL
                     PLPFTLLQNALFLVRLWTVYLLVRLITPPFRVTRATEALAFFARPFSRIPLLFQPFLL
                     LALHGLIALALTRTTELRILTRLTETPAATDVSPFLTGPLLLQLLKTGWLAVLSFSDG
                     LIFMTRSLFVLIIGSLGAAIIQSRGLAVLCSEAVDLLLGRFARRCTGAMGFDFTPLIY
                     FFVVDFLYNGICRGLYMLIHSPLFN"
     gene            8856..11267
                     /locus_tag="PK576_03455"
     CDS             8856..11267
                     /locus_tag="PK576_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60677.1"
                     /translation="MKKMMLSIAGVMSALAVSAVNISADIAKAAYYTNATVITLTNSQ
                     DIMISGSGTIDVILIGGGGGGGANRVDLSQGGGGGGGGGVILRRDVAVSAGVYPIEIG
                     SGGTTGVIGNATGTADNGGNTTAFGITAQGGGGGANYSNNGQARQGRAGASGGGSTAY
                     NATAVGGASIAAQGEYGNDGGGSNHLYGPGGGGGAGAPGVTSGGSAPGGGGDGIELAV
                     YGDYGVYYGGGGAGYRQGTFASGGKGGGGSRYEKGVDGLGGGGSGGYDGGCGAVVIKF
                     TPTPVSDTKDFLLTGGDEKILLTNGTVHVFRQSGKLTVAGSGCVEVLMVGGGGGGGNH
                     TYAGGGGGAGGLVHISSARITAGEYDIIVGAGGAVGANGGDSSFLGALAHGGGAGGYN
                     STGSSGGSGGGGSTISANKVPGGSAVFAIDGNIGDVGGASGANIYAPGGGGGAGGPGE
                     DGVDSTSGTANIPGDGGDGLAFDITGDNVWYAGGGGGGEPNRGAKGGKGGGGDSHVAG
                     VDGLGGGGGGYAAGGDGIVIVRYAKKSFYRDYIENATGGTVTRSEGCNIHTFTEDGTF
                     SLPEAGVVEFLMVGGGGGGGVNLDTDPAQGGAGGGAGGLIYRDVVVLNAGTHQVKIGA
                     GGNVGENGGNTEFVRFVAYGGGHGGGRSGNARKPASGGSGGGGIWISGGAYVPGQAIH
                     GEQGNLGNDGGECSHVYAAAGGGGAGAVGGNSNGTSPGIGGDGLAFDISGTLTYYAGG
                     GSGYRKGSGYPAMSQGGLGGGGPGGQNGVDGLGGGGGGNAAGGSGVVIIRYLLPKKGF
                     VVICK"
     gene            11532..13625
                     /locus_tag="PK576_03460"
     CDS             11532..13625
                     /locus_tag="PK576_03460"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOE60678.1"
                     /translation="MRRLSWLGCVWLAMGCLAADVVLFDAARTPLSAVASHSGGTFTL
                     TDGLLAIETKGNLGYPGVRIAGEWDLSGCNSVTFELEHCDGKGELPLTVRFDNPGADP
                     GKSTGVFVDRIKMPRKGAHACTVTLPPTLPSGRAISSKLTGMRTTPFQTTGVVADLDT
                     ARVVGVAVYMRLNKLDWKWGVRRIVAHTGPVADVPSWMKLTEKEFFPFIDVYGQFKHK
                     TWPGKIHSDEELKTAARREEADLAAHPGPKGWSRFGGWADGPKRAATGHFRVEKIDGK
                     WWMVDPDGYLYWSHGPVRVTPSTAITPLDNREFYFEGLPPREGSPFSLFYTTRDELLY
                     PYYVKRNIQRTYDFSAANLLRKYGGTWFETFADLAHRRLRSWGMNTIANSSDKKICLM
                     DRTPYTDRFELKSKPIAGSHDGWWPFRDPFDPSFRADVRRLMNEHKAEMQDPWCFGFF
                     VDNELSWGGETDLAAWTLESPADQAAKRVFRERMRKAYGTIDKLNAAWGTAYADWEAF
                     LTTRTRPGKSAEPDLKAFTLEIADAYFRNIREEFKAAAPNKLYMGCRFAGRGPEFAVR
                     VAARHCDLISYNIYARHLNDFKLPEGIDKPVIIGEFHFGALDRGLFHPGLIKLENQEE
                     RGRVYVQYVTSALKHPHVVGVHWHQFGDQATTGRFDGENFQVGFVDCCNTPYPETIAG
                     IREVGYRLYEIRSGK"
     gene            13667..14443
                     /locus_tag="PK576_03465"
     CDS             13667..14443
                     /locus_tag="PK576_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE60679.1"
                     /translation="MRFKGKVVLVTGTSRNTGVGIAALFIREGAKVCVCSSSPESTAK
                     GAAELRAMGLTEFTETPADISDTEQVRAMFRVIREKFGRLDILVNNACNQGIGPAFEE
                     MEPAYFLSVVKTNLLGTFQVSQEAVKMMLAQESRGVIVNLGSNVSMRAIRNRTAYVAS
                     KGGLDALTRSMAVDLGPKGIRVNMVAPGYIYTDRWDKLNEATMKRRRLNCPIGMEATA
                     EDIAQAVAFMASDAARNICGERLVVDAGCSAQHMPVDVDI"
     gene            14440..15699
                     /locus_tag="PK576_03470"
     CDS             14440..15699
                     /locus_tag="PK576_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE60680.1"
                     /translation="MKPSKSYKWELLALLSFAFFFHQADRALFSVVLIPIQTELGLTD
                     GQMGEIGSWMFATLAVMVPIAGFMGDRLSRKWIITCSLIFWSCATAMTGMATGVLGLI
                     LFRSVATGGGESFYAPSAYALLASYHKETRSVALSVHQAALYVGLMCSGYLAGLVEAA
                     FGWRSVFYLFGGAGVLLGLVFIFRLKDAPAEPRPDKPAAVNKPSVWESVRVLVKTPSA
                     LLATAGFCAVVFVNNAYVFWAPTFVMEKFKSSATPMTLTQAAGGAMLYHHLAAFGAIM
                     AGGVLTDWAVSRYPRFRLLLQGAALLLGAPMIYWIGVANGITATWIAMAGYGIFRGLF
                     EVNTHASLFDVVPAKFRSTAVGLMTMTAFFLGTVPPKLIGKFCDLHGKVAGIQQGFTV
                     LAFAYVLGAVAIFCSMLFTFNRDRVLE"
     gene            15711..16886
                     /locus_tag="PK576_03475"
     CDS             15711..16886
                     /locus_tag="PK576_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOE60681.1"
                     /translation="MKITGIKTFVCNAYRTNWVFVKVLTDEPGLWGVGEATLEYKEQT
                     IVSACMDLERDLIGKDPRDIEAIWHSNYRDAYWRGGPVLMSAISAIDIALWDILGKSL
                     NAPVWRLLGGCMRDKVPCYANGWFAGAKEPEEFAAKAVEAIETAGWKGLKWDPFGTAY
                     RQLEPEAFRKAMRCVEMLYEAVNGRAEILIEGHGRFDLPTALRIANALEAFDILWFEE
                     PIIPDTIEALADLRSRVKVPLSFGERLYGTWQFRALLEARGADFVQPDVSHAGGITEL
                     RKIAAIAETHHIPFCPHNPSGPVANAATLQLAASQPGFYLLETMASDVPYRREICDEA
                     VTLRDGYMHIPLDRPGIGVEIDEAAIAKYPYQPISLRHYIGTLTEIRPQNATAYFTK"
     gene            16946..18166
                     /locus_tag="PK576_03480"
     CDS             16946..18166
                     /locus_tag="PK576_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE60682.1"
                     /translation="MSERGREKPRRTGFVRTAVRVALGVAAAVCALQGVGPAWLSVVF
                     PSLSPFTALLAAAAGAGSVLLWGAAAVAAVCLFLPRFFCRWICPAGTCFEGVGFRARR
                     RAWVGRVPRIGVGLVLVGTGAAVLGYPLFGWLDPLVIFQAFFAFFRPGDVAWESMAAA
                     GLPLLILMSLLTPGLWCGRLCPLGATQDLLRAAVTWQRADGKDEASRTVRGTGAGRRI
                     FLGLGAGAVYRLLLPPERAAAGAGVIRPPASGTSARFTRLCARCGNCVRACPSHILHG
                     GGVGAGLAGALAPEVRFGENYCPTTCTVCGQVCPTGAIPRFTRQNKFRNPMGCAQVNP
                     AICLLTEGRECGSCVAACPDAALDFAWDPVDLVSRVTVDAKVCTGCGYCEYVCPVADK
                     AIRIRAPGRKEGAG"
     gene            18163..19617
                     /locus_tag="PK576_03485"
     CDS             18163..19617
                     /locus_tag="PK576_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOE60683.1"
                     /translation="MSDTTDVKKTVWERVTTFLRHDLWEMRSSELPWLYRSGVYLLRM
                     GHLVVDGFRRNACSLHAASLTFFTLMGLVPILVLALALARTFGGADLAKEQLDRHLNA
                     WMSQMEAAVDARTAEIAEGDAEKNQVHEEVTKAFSTQVREIADQLFDQVNSISFGTLG
                     GIGAVMLIWSVVGMLGKVEDSFNRIWGVERSRVLVRKLADYLFVIIILPFLIMVVSSM
                     PIAKMITDAMDKTVGGAVSDGVRMVVNSSLLKSGITLTVGTLTFAFLLGFMPNVRVQT
                     GAALVGGLVTLVLFGGWLKLCAMLQVGIAKYSALYGGFAVLPILLMWVYTSWQIILLG
                     SEIAFAVQNRDTYVLEQNASRASLRSRLLLALTLCAEAAHCAKDPAGGPFAAEAFARR
                     RGISSRFVKDILEDLARHGILAEVAGRPGDYLLARCGSSLTAAEIAKALLDEGEPPEA
                     LGLTALSAPLLAFNQRVDERIEEGFGQPVAALNG"
     gene            19652..20818
                     /gene="tgt"
                     /locus_tag="PK576_03490"
     CDS             19652..20818
                     /gene="tgt"
                     /locus_tag="PK576_03490"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE60684.1"
                     /translation="MRQKPGVFEILKEDRHTGARLGRLWTAHGQIDTPVFMPVGTQAT
                     VKALEPRDLTDLGASVILGNTYHLLLRPGMDVVSACGGLHRFMAWDGPILTDSGGFQV
                     FSLNSLRKIRAHGVEFRSHIDGASFFLGPSEAMAVQRTLGSDIAMCFDECIPYPCDEQ
                     YACQSVAKTLSWAALCLKQERAPGQLVFGIVQGGEYTALRERCARELAAMGFDGYAVG
                     GVSVGEPEDVMLRGVEDGVRGLPRERPRYVMGLGDLYQMSESVARGADMFDCVIPTRV
                     ARHGTAYTRTGQYPVKAGLHKADTRPVEEGCDCYCCRTFSRAYVRHLLNVGEILGVRL
                     LTIHNMHRYLGFMREMREAIANDAFGEWRRALAAVLRPDGRKPVRMGARAPQAG"
     gene            20882..21217
                     /gene="yajC"
                     /locus_tag="PK576_03495"
     CDS             20882..21217
                     /gene="yajC"
                     /locus_tag="PK576_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOE60685.1"
                     /translation="MTTGWFWAQAAQQGTGGVGGMLIPMVLVFGLFYFMMIRPQQRKE
                     KERLRMIAELRAGERVMFAGGLIGTISEARETTFVVEIAPKVMVEVARGAVTRVLKEG
                     DVATLEEGR"
     gene            21320..>21688
                     /locus_tag="PK576_03500"
     CDS             21320..>21688
                     /locus_tag="PK576_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60686.1"
                     /translation="MSRNDLWKWLILAFLTVLSAYVVIPPKEKIRLGLDLSGGTSFTV
                     AIDEAKLRANVRAAADTELTDVEVENQVNAVLRDADARAIEVIRNRIDGLGVNEPVIQ
                     GGKNHRIVVQLPGASAEQRDA"
BASE COUNT         3867 a         5869 c         7628 g         4324 t
ORIGIN      
        1 ccggactcgc cacgaatgcc accaccatgc tcgctacgtt cgacggcgta ctggaacgga
       61 tgggggttga cggctcggag agcgacgccc cgccggcaca ggtggcggcg aaggaggagg
      121 gcacaccctt ccgcattcag gactacagcg aggcggccga acggcttgag gcttccgcgc
      181 tgcgtctgac cgaactgctc cagacctttg atcagaccct cggcgccaac agccgcgaag
      241 cgctggcggc acagctcgac cctgttgtcc ggcgcactcg cgcggaaacc gaagcgttgg
      301 ttgaccacct cttccgccgc gcgttgcaac tcacaggcgc cctgctggcg gccgctctgg
      361 cctaccgcct gatagcaagc cgaatatcgc gtacgccgcg cgcgtgagac caggctcagc
      421 cgtctggtaa aggcgaggca ggggaatagg gcggcaaccg ggatgcgcgt ctgctcgttc
      481 gcaggcaaac gaacgcttgc ctgcacggga tggagaacgc aatgacgacg gtatcaagcc
      541 atgcggctcg tgaggacgct cgcccccgaa aacctgcgtc cccctacgta atctgcggat
      601 gaaacccttg aacccctgaa acccgatccc gatagcgatc ccgaccccga tttggatgaa
      661 acccagaaac cagaaaccag aaacccagaa accctgaaac ccaagaaagt taaacaatcg
      721 atcccgatag cgatagcgat cccgaccccg atttggatga aacccagaaa ccagaaacca
      781 gaaacccaga aaccctgaaa cccagaaacc ttgaaaccta agagaggtga aggttcaact
      841 gctcttttta ggttccacga tcaccgagac gccgtcaggg atcacggtga tccgcgctga
      901 agtcccgtgc tcgtgccagg cgagcgccag tgcctcgtcc agcgaagcgg cgggcgtgat
      961 gtgcagagcc cggatcgcgt cggcgtccca gaggtcggtc accagaatca cgcggtgtcg
     1021 gctgaggata cgggcgagaa tttgatactc ccactggtcg ggcggggtga gggcttgcgg
     1081 tacgcgcaac acctccgcca gcagttccgc aggacttcgg gccccggcca gtgcgcggta
     1141 aaaagattcg ccgcctgaac cgtcgcaaca gcctgccacc atgatgatgg tcccgccggg
     1201 cacgcagcac gcttcggctg cggtcatgcc tttgaccgct tgatacagat tctggtcaag
     1261 cgggtagccg ccattgccgg tgatcacgat ttccgcctcg gcgcggggca cggagaccgc
     1321 gcgccgcatg tcgcggcatc cctgttcgtg cgctgcctcg gcatgtcccg ccacagccgt
     1381 gcggatcgtc ttgtcgggat ggagggtgac attgaggatg aacgccagac gcgcctggcg
     1441 cgcggcgaac agcatgtcgc ggtggatggg gttccgctcc agttgccctg cccgcgcatg
     1501 ggggtcggca atgaatgccg cgcagtggtt ggctagaacc gtttcccgtc cggcgatgcc
     1561 gggcagcacg cttttccgcc ctccggaaaa accggcgaaa aagtggggct cgatgaaacc
     1621 ttcggccacc agcagctcgg tctccagcgc cagccggttc aaccagagtg cgccgcccga
     1681 cggcagttga tccagcatcc ggagatttgc cgtgtcggtg cagtcgtgca tcacaaacgt
     1741 ttcgcgcgcc accagttccg cgccgaattt ctcgcgcagc tccgcatccg aggttggccg
     1801 gtggcagccc gtggccacga ggatttggat cgcgatgccg gggttgcccc gccgcaggcg
     1861 ctccagcagg aggggcagcg tgatgcggct gggcaccgga cgggtgtgat cactggtgat
     1921 taggacggcg gaggctttgc ctgcggccag ggtttcgagc cgcggcgtgc cgacgggatg
     1981 atccaacgcc gcgcaaaccc gttggcgctg ttccgcttcc gagggggaga ccgtgcatgc
     2041 ggtgccggtc ggctctaaca gggtgaggcg cacgtttgcg ggaagcgtcg cctccaaatg
     2101 accccggtgg taaggaatgc ggattgtggg catggtattc cgggcgtggt tccggacgct
     2161 tcttttccat gacgaccgta tggacgatcc gttgcgggat tgcggcgtcc ttctgttatg
     2221 aagcaacaag tttatcggtt tggtgcccga atgcaatcgc gttttcagga tcgtccggcc
     2281 tgcgtttccc atgcggcgcg gcttgacgcc gcgcggttcc gcgcctacca taccaggctt
     2341 tcgtttatcg aaatccgcag gcaacggccg tacgtgtgac aggggcatct ttgcattgca
     2401 gtcggagcgg cgtcgcttta tacttctgag taacgattgc agttcaaacg gaacctaaca
     2461 tgggagaacg cggcagcatg gcttatcagc atattcaaat tccctctttc ggtgaaccga
     2521 tcacccgagg cgacgacggg cgtctgcatg tgccggattg tccggtcatc ccgtttatcg
     2581 agggtgacgg cacggggccg gacatcacgc gtgcggcacg gatcgcatgg gatgccgcgg
     2641 tgcagacggc gtatggcgaa aaacggaaaa tcgcctggat ggagatctat gccggggaaa
     2701 aggcgaatgc ggtgtacggt cccggcacct ggttgcctga tgaaacgctg gaagcctgca
     2761 aggcgtacct cgtctcgatc aagggcccgc tgaccacgcc ggtgggcggc ggcatccgtt
     2821 cgcttaatgt ggcgttgcgg caggagctgg acctttatgt ctgcctgcgt ccggtgcgct
     2881 ggttccaagg cgtgccctcg ccggtgaaac ggcccgagct gaccgacatg gtgatctttc
     2941 gtgaaaatac ggaagacatc tatgccggga tcgaatggct ggcggagagc gacgaagcgc
     3001 gcaaggtcat cgcgttcctg caaaacgaga tgggggtcaa gaagatccgc ttccccggga
     3061 gttcgtcgat cggcatcaag ccggtttcca tagaggggac gcggcgattg gtgaaggcgg
     3121 cgcttgatta cgcgatcgac aatgaccgct cgagcgtcac gctggtccac aagggcaaca
     3181 tcatgaagtt cacggagggc gggtttcggg actggggcta ccagctcgcc cgcgaggcgt
     3241 acggtgccgc gctcatcggt gacggaccgt ggtgccggtt caaaaacccg aaaaccggcc
     3301 gggagattac ggttaaggat tgcatcgcgg atgcgttcct gcagcagatc ctgacccgcc
     3361 ccgcagaata cgatgtgatc gccacgctca atctgaatgg cgattacatt tccgatgcgc
     3421 tggcagcgtg tgtgggcggg atcggtatcg cgccgggcgg gaacatcaac tacacgaccg
     3481 gggtggcggt attcgaggcc acgcacggca cggctccgaa atacgccaat ctggacaagg
     3541 tcaatcccgg ctcgttgatc ctctcgggtg agatgatgct gcgttacatg ggatggaccg
     3601 aggcggcgga caaactgatc agcgccatgg accgcgtgat cgcgaataag acggtgacct
     3661 acgatttcgc gcgcctcatg gaaggggcgg ttgaagtctc ttgcagtgcg ttcggccgcg
     3721 cgctggccga cgcgatgcaa taggcatgag cgcaacaaac gcaataaaca agtcgttttt
     3781 catgcgaagg gtgcgaatgg gaagggtttg tcatgaaaaa acctttgtgc cttagtgtga
     3841 tatgaaaagg gtttttaggc tgaaaatgcg catggacaaa aggaaggcgg atgaaatgag
     3901 tcggagccag tatggtgcgg tcctgttcct gttgtttgcg ggatggatcg ttgcggccgg
     3961 gtgcggcaaa caggaaacca cggctgcgga cggatcgcgg ccggtgaagc ttacgtacag
     4021 cgttttcttc ccgcccacgc acaaacaggc gaaactcgcc gaggcgtggg ccgaagagat
     4081 cgggcgtcgc acacagggca aggtccggat cacggtcttt tgcggcggct cgctgacgaa
     4141 ggctgaccag tgctatcagg gtgtggtgga tggcatctcc gatatcggca tgagcgcctt
     4201 cgcgtacacg cgcggacggt ttcctttgct ggaggggttg gatctgcctg tgggatatgc
     4261 ggacggggtc agcgcgacac ggatcgtgaa cgaactctgc cacgcctttt taccgaaaga
     4321 gatccgcgat gtgaaactgc tttacgtgca tgcgcacggc cccggtattc tggcctccaa
     4381 acgtccgatg cgcacgctcg ccgacttgaa gggggccaag gttcgggcga ccgggttctg
     4441 cgcgaaagtg gtggagagtc tgggcggttc gccggtgagc atgccgcagg gcgatacgta
     4501 cgaggcgctc cagaaaggcg tggtcgatgc caccttctgc ccggtcgaga cgctgaaggg
     4561 atggaaacag ggggaggtga tccgcaatat caccgactcg cgctgtatcg ggtataccac
     4621 ctcctttttc gtggtgatca acccggtgcg ctgggcctcg ctgccggatg aggtgaagca
     4681 gatcatggaa gatgtcagca aggagtgggt ggagcgccac gctcaggcgt ggaacgaggc
     4741 ggatgacgag gggctggcct ttgcccggtc gctggatcgg gaggtggttc cgctcgatcc
     4801 gtcggagcag gtgttgtggc ggaaacaggt cgagccgctt ctgcgggagt atgcggaacg
     4861 tgcagaagcg aagggcctcc ccggcaagga ttttctgaac cggctcacca ctgcggtccg
     4921 ggaggctcag gagggctcct cacgatgagt cctgcacaaa aaccggtccg aaagccgttg
     4981 ggtccttttt ggcagtcggt ggtcggcctc tgccggttca tgacgggaat cgcctgttgc
     5041 ggattggcgg tcatgaccgc gttggtgtgc gtcgacgtgg tgctgcggct tgcgggtcat
     5101 ccggtcaaag gtgtctatga tctggtgcgt attgtcgccg cggtgacggt cgcctgttcc
     5161 ctgccgatca ccaccgccaa gaaggggcat gtggcgatcg agtatttctt cctgaagttg
     5221 aatcgcgtgg gccggcttgt ggtggattcc gtcatgcggc ttctgatgat cgcggcgttt
     5281 ttactcaccg ctacggaatg cgtgggctat gggttgcgct tcctgcgcaa ccggcaggtt
     5341 tcggatacga tcgaggtgcc gatcttctgg gtgccgtggg tgatggccgt ggcgtttgcc
     5401 gtgacggcct tggtggtggt tttccatttg gtctatcccg gacgggagat ggtgaagtca
     5461 tgacgcctgt gatgttcggt gtggtcgggt gtgtcgcgct gttcatcttg ctgctctgca
     5521 gcatgcccgt cgcgatcgtc atggggttgg tcggcgttgt cggatatgcc gccgtggtct
     5581 ccggcaaggc ggcgctggcc atgctttcgg cggatctgta cgatgtgttc tccagttaca
     5641 atctgaccgt gatcccgctt ttcatcttca tgggacaggt ggcgtttcac actgggatca
     5701 gcgggaggtt gttcacggcg gccaacagct ggctctccgg gctgaaaggc gggttggcta
     5761 tggcgaccgt cggtgcctgc gcggccttcg gcgcgatctg cggctccgga ccggccactg
     5821 ccgcgaccat ggctgcggtg gcattgcccg agatgcggaa gcacaagtat gatgacgccc
     5881 tggccagcgg tacggttgcg tcagccggcg ggcttgggat gctgatcccc ccgagtgtgg
     5941 ttttcatcgt gtacgggatt ctcaccgagc agtccatcgg caagctcttc atcgcgggca
     6001 ttcttccggg gattgtggtg gcgctgctct tctgtctgac gatccatgtt ctgtgcttgg
     6061 ccaaccctgc gttggccccg aaagcggtgc ggaccccgtg gcgggtgcgt ttccgttcgc
     6121 tctccggggt tacggagacc atgatcctct ttgttctggt gatgggcggg atgtttgcgg
     6181 gatggttcac gcctacggag ggcgcggcga tcggtgcggc tggcagtgtg gcgatcgcgg
     6241 tatgcggcgg acacggatcg tgggccctgt tccggcaggc cgcgcaggag accatccgga
     6301 cctcatgtat ggtgctggtg attgtggcgg gcgccacgat gttcggccat tttctggcgg
     6361 ttacgggact gcccacgcag ttcgccgcct ggcttgcggc cctgccgttg ccgtcatggg
     6421 tggtgatgct cctcatcctt ttcttttatc tgctggcggg ctgttttgtc gactcgctgg
     6481 cgctgatcct tctgaccatt ccgattttct atccggtggt catggggttg gggttcgatc
     6541 caatctggtt cggggtcatg atcgtgctgg tgacgcagat gggggtgatc accccgccgg
     6601 tgggggtcaa cgtctatgtc gtgagcggta tcgcacggga tatcccgctc cagacggttt
     6661 tccgcggtgc gatcccgttc ctgttcgcgc tgatcgtggc gtgcgggatc ctgatgcttt
     6721 ttcccggtct ggccacatgg ctgcccgggg tggtgcgata gtcaattcgg gaaggaggac
     6781 gtatggacgg atcaacggca cggcacggaa attaccggaa gattctgttt tgcacggatt
     6841 tctccgcgaa tgcggatttt gcgtttcatt tcgcggtgga tgccgccctg cggaataccg
     6901 ggtctacact ctacctgctg cacgtcattc ccgagccgga cgcgcaattt tggaaggggt
     6961 acatttacga ggtcggcgat atggacgcga aggcgcgcgc cgatatcgac cgtatgattg
     7021 atacggttta ccgcccgcgc gttccgtccg atgtcacgtt tcagattgcg atgcggatcg
     7081 gcgacgccgc ccgcatgatt ctggatttcg ccgcatccca agatgtggat ctgatcgttc
     7141 tcgggaggca gggacgaggc gccatcacgc agtggttgct ggggaatgtg accggtaaag
     7201 tggtccgcag aagcacatgc cccgtgttgg ttgtgccgca ggctttcaaa acccggcagt
     7261 cggaacctgc cgcggcggac gagcagtgaa cgggcggcgc gtgccgccgg gcctcattgt
     7321 tttgcgaagg cctgacgcgc ggccgcggcg gtcacgccgc agagggccac gcgtttgagc
     7381 cgtgaggtct cgccggacaa aagcacgacg gatcgtttgg gcacgcccgc cactttggcg
     7441 agaaacgcga tcagggcggc attggccttg ccatccaccg gcggggcctg caaacgcacg
     7501 cgcagccaat ccgcatcgac cccggcgatc tcggaacggt ttgcccgggg cgtggccttg
     7561 accgtcacga cgcacccgtc acgggtttca acgacccaac tcatcttcac ctcagttgaa
     7621 taatggggaa tggatcagca tgtacagtcc gcggcagatg ccgttgtaca ggaaatccac
     7681 aacaaagaaa tagatgagcg gcgtgaagtc gaagcccatc gcgccggtgc agcgccgggc
     7741 gaagcggccc agcaggagat ccacggcctc gctgcataaa acggcgaggc cgcgcgattg
     7801 gatgatggcc gcaccgaggc tgccgatgat cagcacaaag agcgagcgcg tcataaatat
     7861 gaggccgtcg gaaaacgaga ggaccgcgag ccagcctgtt ttcaggagct gcaggaggag
     7921 ggggccggtc aggaaaggcg agacgtcggt cgcggcgggc gtttccgtca gccgggtcag
     7981 gatccgcagt tcggttgtgc gggtgagagc cagggcgatc aacccgtgaa gcgccagcag
     8041 caggaatggc tggaacagga gcgggatacg ggagaacggc cgtgcgaaga aggccagggc
     8101 ttcggtcgcg cgggtgacgc ggaagggggg tgtgatcagg cggaccagca gataaacggt
     8161 ccagagacgc accagaaaca gagcgttttg gagcagggtg aacgggagcg gcaggagggg
     8221 gttgtcggat acggcgggcg gcgtgaaatg aaaggtcgtg ccgagggtca cacccaacgg
     8281 aaactggagc cgtgcggcca gcagcgtctg caacaggacc acgacaatca agatgaccag
     8341 tgcggtcatc gcctcgggta tcgcgagcac tgggcgcagg aacaggatga cacgatcggt
     8401 cagattgatg gtgcccgaca cgaacggatt gaaataaaac tcgcgctccg gtgcggacca
     8461 gaaccgcagc cagaacagca gaaaaacaaa atggaagaga aggaacagat aaaacatagg
     8521 acaccgtgga gcgtttcccg tatgacggaa acaagcgtaa caggagacgt agggcgtgtc
     8581 gagtgtaaca actcatcggg ggcggcacgg tgatgcgatc cgcctgacca gacaacgctt
     8641 cgtgggagcg ggggacaaac cgggagaaac tgatcttcgt tagacgagac cgcaaacagg
     8701 ggggggcacg aaaacgccgg gcgtaaactc gacaatccgc aacccgaatg ttatattttg
     8761 gacaatccga gaccaaagtg gtttttttag tgcgttttgt tctccctcgt tctctgggaa
     8821 gggccggggt gaggggtaca cgaaaggttg tgatgatgaa aaaaatgatg ctctcgattg
     8881 ccggtgtgat gtccgcgctg gccgtttcgg cggtgaatat ctccgcagat atcgcgaagg
     8941 cggcgtacta taccaacgcg acggtgatca cgctcaccaa ttcgcaggat atcatgatca
     9001 gcggttccgg tacgatcgac gtcatcctca tcggcggcgg tggtggcggt ggcgcgaaca
     9061 gggtcgatct ctctcaaggc ggcggcggcg gtggcggcgg aggagtgatt ctgcgacggg
     9121 atgtcgcggt ctccgccggt gtttacccta tcgaaatcgg ctcgggcggt actacgggcg
     9181 tgatcggtaa tgcgaccgga accgccgata acggtggcaa cacaacggct ttcggtatta
     9241 cggcccaggg tggcggaggc ggcgcgaatt acagcaacaa cggtcaggct cggcaagggc
     9301 gggccggggc ttcgggcggc ggttcaacgg cttacaacgc tacggccgtg ggcggcgctt
     9361 cgattgcggc gcaaggcgaa tacggtaatg acggcggcgg cagcaatcat ctttacggac
     9421 ctggcggcgg cggcggagcc ggtgctcccg gcgtaacgag cggcggatcg gctccgggcg
     9481 gtggcggcga tggtatcgag cttgcggttt acggtgatta cggtgtctac tacggtggcg
     9541 gcggtgctgg ctacagacaa gggacgtttg cgtccggggg caaaggcggc ggcggttccc
     9601 gctatgagaa gggcgtcgac ggactcggcg gcggcggttc tggcggctat gatggcggat
     9661 gtggcgcggt tgtcatcaaa tttacgccga ctccggtttc agacacgaaa gattttctgc
     9721 tgacgggcgg tgacgaaaag atactgctca ccaatggaac cgtgcacgtc ttccgtcaga
     9781 gcggcaagtt gaccgttgcc ggctccggtt gcgtcgaggt gctgatggtc ggcggcggcg
     9841 gcggtggcgg taatcatacc tacgccggcg gcggtggcgg cgccggaggt ctcgtgcata
     9901 tttcgtctgc acgtataacc gcaggcgaat acgacatcat cgtcggtgcc ggtggcgcgg
     9961 ttggtgcgaa cggcggtgac agttcgttcc ttggagcgtt agcccacggc ggcggtgccg
    10021 gtggttacaa cagcaccgga tcttcaggtg gcagtggcgg cggcggttct accatttccg
    10081 caaataaggt gcccggcggt tccgcagttt ttgccataga cggaaacatc ggcgatgtcg
    10141 gcggtgcgag tggtgccaac atctacgctc ccggcggcgg cggcggagcc ggcggccccg
    10201 gggaagacgg cgtcgacagt accagcggga ccgccaatat ccccggtgac ggtggcgacg
    10261 gactcgcctt cgacatcacc ggtgacaacg tctggtatgc gggcggcggc ggtggcggag
    10321 agccgaaccg cggggcgaag ggcggcaagg gcggcggcgg tgattcacac gtcgcgggcg
    10381 ttgacggact tggcggcggc ggcggcgggt atgccgcggg cggcgacggt atcgtgatcg
    10441 tccgttacgc gaagaaatca ttttatcgcg actacatcga gaacgcgacg ggcggaaccg
    10501 ttacgcgcag tgagggctgt aatatccaca ccttcactga ggacggaacc ttctcactgc
    10561 cggaagccgg tgtggtggaa ttcctgatgg tcggtggcgg tggcggcggc ggagtgaatc
    10621 tcgataccga tcccgctcaa ggtggcgcgg gcggtggtgc gggcggtctc atctaccgtg
    10681 atgtcgtggt gctcaatgcc ggaacccatc aggtgaagat cggtgccggc ggtaatgtgg
    10741 gcgagaacgg cggcaatacc gagttcgtca ggttcgtcgc ctacggtggc ggacacggcg
    10801 gcggtcgtag cggaaacgcc cggaaacctg catccggcgg cagcggcggc ggcggaatct
    10861 ggatcagcgg aggagcgtat gtgcccggac aggcgattca cggggagcag gggaacctcg
    10921 gcaatgacgg cggcgagtgt tctcatgttt atgcggcggc cggcggcggc ggagccggtg
    10981 cggtcggcgg gaacagtaac ggcaccagcc ctggtattgg cggggacggt ctggctttcg
    11041 atatttccgg tacgctgacc tattatgctg gcggcggatc cggttaccgt aaaggcagcg
    11101 gttatccggc gatgtctcaa ggcggactcg gcggcggcgg accgggcgga cagaacggtg
    11161 tcgacggact cggcggcggc ggcggcggta atgccgcagg cggcagcggc gtcgtgatca
    11221 tccgctatct tctgccgaag aagggtttcg ttgtgatttg caaatgaccg tgacctgcgg
    11281 catccggcct gtttggcagg acaacggatc cgactgatcc gtccggtccg gctggtccgc
    11341 cattgcggtc ggggatgccc gcgctttcga ctcccgcgcc aatgcggatg gcagggaacg
    11401 tgtcggaacc cgatccccat ggttcggaaa atcgaggggg tgggttcgga ttatcgagat
    11461 tgcggaaacg gctccgggtg cggtatagta ccgtatggtt ttgccgtgtt agaaaagata
    11521 aggggctcga tatgcgacgt ttgagttggt tggggtgcgt atggctggcg atgggatgtc
    11581 tggcggcgga tgtggtgttg ttcgatgccg caaggacgcc gctgtcggcg gtcgcgtcac
    11641 attcgggcgg aacgttcacg ctcacggacg ggttgctcgc gattgagaca aaaggcaact
    11701 tgggttatcc cggggtacgc atcgctggcg aatgggatct gtcgggctgc aacagcgtca
    11761 ccttcgagtt ggagcactgc gacgggaagg gggagttgcc gcttacggtt cgctttgaca
    11821 atccgggcgc ggatcccggc aagtccactg gcgtgtttgt tgaccgcatc aaaatgccgc
    11881 gcaagggcgc gcacgcctgt acggtcacac tgccgccgac actgccttcg gggcgtgcga
    11941 tcagcagcaa gctgaccggc atgcgcacca cgccgtttca aacgactggc gtggttgcgg
    12001 atctggacac cgcgcgcgtg gtcggcgtgg cggtttacat gcggctgaac aagctggatt
    12061 ggaagtgggg tgtccggcga atcgtggcgc acaccgggcc ggtcgcggac gttccgtctt
    12121 ggatgaaact gacggagaag gagttcttcc ccttcatcga cgtgtacggt cagtttaagc
    12181 ataaaacgtg gccgggaaag atccattccg acgaggagct gaagacggcg gcacggcgtg
    12241 aagaggcgga cttggcagcc catccggggc cgaagggctg gagccgtttc ggtggatggg
    12301 cggacgggcc gaaacgtgcg gcgaccgggc atttccgcgt cgagaagatc gacggcaagt
    12361 ggtggatggt ggaccccgat ggatatctgt actggtcgca cgggccggtg cgcgtgacgc
    12421 cttcgaccgc gatcacgccg ttggacaacc gggagttcta tttcgagggg ctgccgccgc
    12481 gcgagggatc gcctttttcg ctcttctaca ccacgcgcga cgagttgctc tatccctatt
    12541 acgtcaaacg caacatccag cggacttacg atttctcggc agcgaatctg ctgcgcaaat
    12601 atggcggaac ctggttcgag acctttgcgg atctcgcgca ccggcggttg cggagctggg
    12661 gcatgaacac gatcgcgaac agttcggata aaaagatttg cctgatggac cgcacgccct
    12721 ataccgaccg gttcgaactc aaatccaagc ccatcgcggg gagtcatgac ggctggtggc
    12781 ccttccgtga cccgttcgat ccctctttcc gggcggatgt ccggcggctg atgaacgagc
    12841 acaaagccga gatgcaagat ccgtggtgct tcggcttttt cgtggacaac gagctgagtt
    12901 ggggcggcga gaccgatctg gcggcgtgga cgctggagtc gccggcggat caggcggcca
    12961 agcgcgtgtt ccgcgagcgc atgcggaagg cctacggcac cattgacaaa ctcaacgcgg
    13021 cgtggggcac ggcgtatgcg gattgggagg cattcctgac gacccgcacg cgtccgggca
    13081 aatcggcgga accggatctc aaggcattca cgctggagat cgcggacgcc tacttccgga
    13141 atatccgcga ggagttcaag gctgcggctc caaacaagct ctacatgggg tgccgcttcg
    13201 cgggacgggg tcccgagttc gcggtgcgcg tggcggcccg gcactgcgac ttgatcagct
    13261 acaacatcta cgcgcgccac ctcaatgact tcaagttgcc ggagggtatt gataagccgg
    13321 tgatcatcgg cgagttccat ttcggggcgc tggatcgcgg ccttttccat cccggtctga
    13381 tcaagctgga aaatcaggaa gagcgcggcc gggtctatgt gcagtatgtc acgtcggcat
    13441 taaaacatcc gcacgttgtc ggcgtgcact ggcaccagtt cggcgatcag gccacgacgg
    13501 gccgcttcga cggtgagaat tttcaggtgg ggtttgtgga ttgctgcaac acgccgtatc
    13561 ccgagacgat cgcggggatc cgtgaggtgg gataccggtt gtacgaaatc cgtagcggaa
    13621 aatgaggcgt gaattgacaa tgggtgaaac cagaaaggca gaagggatgc gttttaaagg
    13681 caaagttgta ctggtgaccg gaaccagccg gaacacgggt gtgggaatcg ccgcgctttt
    13741 tatccgcgag ggagcgaaag tctgcgtgtg cagttcatct ccggaaagca cggccaaggg
    13801 tgcggcagag ttgcgcgcga tgggtttgac cgagttcacc gagacgccgg ccgatatttc
    13861 ggataccgaa caggtccgcg ccatgttccg cgtgatccgg gagaagttcg ggcgattgga
    13921 cattctggtg aacaacgcct gcaaccaagg cattgggccg gcgttcgagg agatggagcc
    13981 cgcgtatttc ctgtcggttg tcaaaaccaa cctcttgggc accttccagg tttcgcagga
    14041 agcggtcaag atgatgctgg cgcaggagag tcgcggcgtg atcgtcaatc tcggctcgaa
    14101 cgtctcgatg cgggcgatcc ggaaccgcac ggcctatgtg gcaagcaagg gcgggttgga
    14161 tgcgctgacc cgctcgatgg cggtggatct cggccccaag gggatccgcg tgaacatggt
    14221 cgcgccgggg tatatctata ccgaccgttg ggataaactc aacgaggcga ccatgaagcg
    14281 ccgccgcttg aactgcccga tcggcatgga ggcgacggcc gaggacatcg cgcaggccgt
    14341 ggctttcatg gcttcggacg ccgcgcgcaa catttgcggt gaacgtctgg tggtggatgc
    14401 cgggtgcagc gcgcaacaca tgccggtgga tgtggatata tgaaaccttc aaaaagctac
    14461 aagtgggagt tgctggcgct gctgtcgttt gctttctttt tccatcaggc agaccgtgcc
    14521 ctgttcagcg ttgtgttgat cccgattcaa acggaacttg gcctgaccga cggccagatg
    14581 ggcgaaatcg gttcatggat gttcgcgacg ttggcggtga tggttccgat agccgggttt
    14641 atgggcgacc gcctgagccg taagtggatt atcacatgca gcctgatttt ttggagctgc
    14701 gcgacggcca tgacgggaat ggccacgggg gtgctcggcc tgattctctt ccgcagtgtg
    14761 gcgacgggcg gtggcgagtc cttttatgcg ccgtccgctt acgcgctgtt ggcgtcgtac
    14821 cacaaggaaa cgcgttccgt ggcgctttcc gtccatcagg ccgcgttgta tgtcgggctg
    14881 atgtgcagcg gctatctggc tggtttggtc gaggcggcct ttggctggcg gtcggtcttt
    14941 tatctgttcg gtggtgccgg tgtccttctc ggccttgttt tcatcttccg gctgaaggac
    15001 gcgcctgccg agccccggcc ggacaagcct gccgccgtca ataagccgtc ggtctgggaa
    15061 tcggtccgcg tgctggtgaa gacgcccagc gcgctgttgg cgacagccgg gttctgcgcc
    15121 gtggttttcg tgaacaatgc ctacgttttc tgggcgccga cctttgtgat ggagaagttc
    15181 aagagttccg cgacgccgat gacgctcacg caggcggcgg gcggggcgat gctctatcac
    15241 cacttggcgg cgttcggggc gatcatggcg ggcggggtgc tgaccgattg ggccgtgtca
    15301 cgctatccgc gttttcgttt gttgctgcag ggtgcggcgt tgttgctggg ggcgccgatg
    15361 atctactgga tcggcgtggc gaacggcatc acggccacat ggatcgcaat ggcgggatac
    15421 gggattttcc gcggtctctt cgaggtcaac acccatgcgt cattgttcga cgtggtgccg
    15481 gcgaaattcc gttccaccgc agtcgggctg atgacgatga cggcgttctt tctgggcacg
    15541 gtgccgccca agctgattgg caagttctgc gacctgcacg gcaaggtggc cggtatccag
    15601 caggggttca cggtgttggc gtttgcctac gttctggggg cggtcgcgat attctgttcg
    15661 atgctgttca cgttcaaccg tgaccgcgtg ttagagtagg acacggtttt atgaaaatca
    15721 ctggaatcaa aacgtttgtc tgcaacgcgt accgcaccaa ctgggtgttt gtcaaagtgt
    15781 tgacagacga gccgggcttg tggggagtcg gcgaagccac tctggagtat aaggagcaga
    15841 cgatcgtatc tgcctgcatg gatctggagc gtgacctgat cggcaaggac ccgcgcgaca
    15901 tcgaagcgat ctggcacagc aattaccgcg acgcctactg gcgcggcggg ccggtgttga
    15961 tgagcgcgat ctcggcgatt gatatcgcgc tgtgggatat tttgggcaag agtctgaatg
    16021 cgccggtctg gcggctgttg ggcgggtgca tgcgcgacaa ggtgccctgt tatgccaacg
    16081 gctggttcgc aggcgcgaaa gagccggaag agttcgctgc gaaggccgtc gaggcgatag
    16141 agacggctgg ctggaagggg ttgaagtggg acccgttcgg taccgcctac cggcagcttg
    16201 agccggaggc gttccgcaag gcgatgcgct gcgtcgaaat gctctatgag gcggtcaacg
    16261 gccgcgcgga gattttgatc gaggggcacg ggcgcttcga tctgccgact gcgctgcgca
    16321 ttgccaatgc gctggaggcg ttcgacatcc tctggttcga ggagccgatc attccggaca
    16381 cgatcgaggc acttgccgac ctgcgcagcc gcgtcaaggt gccgctctcc ttcggcgaac
    16441 gcctctacgg aacttggcag tttcgtgcgt tgctggaggc gcgcggcgcg gacttcgtgc
    16501 agccggacgt ctcgcacgcg ggagggatca ccgagttacg taagattgcg gcgattgccg
    16561 agacccatca catcccgttt tgtccgcaca atcccagcgg gccggtggcg aatgccgcca
    16621 cgctgcaact cgccgcgtcg cagcccgggt tttatctgct ggagaccatg gcgagcgatg
    16681 tgccgtaccg ccgcgagatc tgcgatgagg ccgtcacgct gcgcgacggg tacatgcaca
    16741 ttccccttga tcgtccgggg atcggcgtgg agatcgacga ggcggcgatt gcgaaatatc
    16801 cctatcagcc aatctcgctt cgccactata tcggcacgct caccgaaatc cgtccgcaga
    16861 acgcgaccgc ctatttcacg aagtgacgcg ttctccgaat gcgccggacg ttgcgctctg
    16921 cgcgaagtgt cggtatgata cgggtatgtc agagcgagga agggagaagc cgcggcgcac
    16981 ggggttcgtg cgcacggcgg tgcgggtcgc attgggtgtg gcggctgcgg tgtgcgccct
    17041 gcagggggtc ggtcctgctt ggctgtcggt ggtgttcccc tcgctgagtc ccttcacggc
    17101 actgctcgcg gcggcggccg gtgccggcag tgttttgctg tggggcgcgg cggcggtggc
    17161 tgccgtgtgt cttttcctcc cgcgtttctt ttgccgctgg atctgtccgg caggcacctg
    17221 tttcgagggg gttggattcc gtgcgcggcg gcgggcgtgg gtggggcggg tgccgcggat
    17281 cggggtgggg ctggtgctgg ttggcacagg ggctgcggtg ttggggtatc cgctgttcgg
    17341 gtggttggat ccgctggtga tttttcaggc gtttttcgct ttcttccggc caggcgacgt
    17401 cgcgtgggag agcatggcgg cagcgggttt gccgctgctg atcctgatgt cgcttctgac
    17461 gcccggcctc tggtgcggca ggctctgccc gttgggtgcg acacaggatt tgctgcgcgc
    17521 cgcggtcaca tggcaacggg cggacggcaa ggacgaggcg tcccgcacgg tacgcggtac
    17581 gggggcgggg cgcaggattt ttttggggtt gggcgcgggt gcggtctacc ggctgttgtt
    17641 gccgccggaa cgcgcggcgg caggggccgg ggtaatccgt ccgcccgcga gcgggacctc
    17701 cgcgcggttt acgcggttgt gcgcgcgctg cggcaactgc gtgcgggcgt gccccagcca
    17761 tattttgcat ggcggcgggg tgggtgccgg actggccggc gctctggcgc cggaagtccg
    17821 gttcggggag aattactgcc ccaccacctg cacggtatgc gggcaggtct gccccaccgg
    17881 tgcgatccct cggtttacgc ggcaaaataa attccggaat ccgatggggt gcgcgcaggt
    17941 gaaccccgcg atatgcctgc tgacggaagg gcgtgaatgc ggatcgtgtg tggcggcctg
    18001 tccggatgcg gccttggatt tcgcttggga tccggttgat ctggtgagcc gggtgacggt
    18061 agatgcgaaa gtgtgcacgg ggtgcgggta ttgcgagtat gtctgtccgg tggctgacaa
    18121 ggcgatccgg atccgtgcgc ccgggcgaaa ggagggagca ggatgagtga caccacggac
    18181 gtgaagaaga cggtgtggga acgggtgacg acgtttctgc gtcacgactt atgggagatg
    18241 cgctccagtg aactgccttg gttgtaccgg agcggggtgt acctgctacg gatggggcac
    18301 ttggtcgtgg acggcttcag gcggaacgcc tgttcgttgc atgcggcctc gttgaccttt
    18361 ttcacgctga tggggctggt tccgattctg gtgctggctt tggcgttggc gcggaccttc
    18421 ggcggcgcgg atctggccaa agagcagctg gatcgtcatc tcaacgcgtg gatgtcgcag
    18481 atggaggcgg cggtggatgc ccggaccgca gagattgcgg agggggacgc ggaaaaaaac
    18541 caagtccacg aagaagtgac gaaagccttt tcaactcagg tcagggagat cgcggaccag
    18601 ttgttcgatc aggtgaactc catcagtttc ggaacgctgg gggggatcgg cgcggttatg
    18661 ttgatctggt ccgtggtggg catgctcggc aaggtcgagg atagtttcaa tcgaatctgg
    18721 ggcgtcgaac gttcgcgcgt gctggtccgc aaactggcag actacctgtt cgtcatcatc
    18781 atcctgccct ttctgatcat ggtggtttcc tccatgccga tcgcgaagat gatcacggac
    18841 gcaatggaca agactgtggg gggggcggtc tccgatgggg tccggatggt tgtgaacagc
    18901 agtctgctga agagcgggat cacgctgacg gtcggcacac tgacgttcgc ctttctgctg
    18961 gggttcatgc cgaatgtgcg ggttcagacc ggcgcggcgc tggtgggtgg gctggtgacg
    19021 ctggttctgt tcggcggctg gctcaaactg tgtgcgatgc tgcaggtggg gatcgccaaa
    19081 tacagcgcgc tgtacggcgg gttcgcggtg ctgccgattc tgctcatgtg ggtatacacg
    19141 agttggcaaa tcattttgtt gggctccgag attgcctttg cggtgcagaa ccgcgacacc
    19201 tatgtgctgg agcagaatgc gtcgcgggcc agtctgcgtt cgcgcctgct gttggcgctg
    19261 accctctgtg ccgaggccgc ccattgcgcc aaggatccgg cgggcgggcc gttcgcggcc
    19321 gaggcgttcg cccggcgtcg ggggatttcc agccgctttg tcaaagacat ccttgaggat
    19381 ctcgcgaggc acggcattct ggccgaggtg gcgggacggc cgggcgacta tctgctcgcc
    19441 cgctgcggca gcagcctcac ggctgcggag atcgctaaag cgctgctgga cgagggcgag
    19501 ccgcccgagg cgttggggtt gacggctctt agtgcgccgc tgcttgcctt caaccagcgg
    19561 gttgacgaac ggatcgaaga agggtttggg cagcccgttg cagccttgaa cggatgatcc
    19621 gcaagggagt gtgggagttt ttgtggaacc gatgcgacag aagccggggg tgttcgagat
    19681 tcttaaagag gaccgccata cgggtgcgcg gctgggacgg ctgtggaccg cgcacggtca
    19741 gatcgacacg ccggtcttca tgccggtggg gacgcaggct acggttaagg cgctggagcc
    19801 gcgcgacctc acggatttgg gcgcgtccgt gatcttgggc aacacttacc atctgctgct
    19861 gcggcccggc atggatgtcg tgtccgcctg cggcggtctg caccggttca tggcgtggga
    19921 cggccccatt ctgactgaca gtggcggatt ccaggttttc agcctcaact ccctgcgcaa
    19981 aatccgcgcg cacggcgttg aattccgttc gcacatcgac ggggcgtcgt tctttctggg
    20041 gccgtccgaa gccatggctg tgcagcgcac gctgggttcg gatatcgcga tgtgttttga
    20101 cgagtgcatc ccgtacccct gtgacgaaca atacgcttgc cagtctgtgg cgaagaccct
    20161 atcatgggcc gctctctgcc taaagcagga acgcgcgccg ggacagctcg tgttcggcat
    20221 tgtgcagggc ggggagtaca ctgccctgcg tgaacggtgc gcgcgggaac tggcggccat
    20281 gggctttgac gggtatgcgg tcggcggtgt cagcgtaggc gagcccgagg acgtgatgtt
    20341 gcggggagtg gaggacggcg tgcgcggctt gccgcgcgag cggccgcgtt atgtgatggg
    20401 gctgggtgat ctgtatcaga tgagcgaatc ggtggcgcgc ggggcggaca tgttcgactg
    20461 tgtgatcccg acgcgtgtgg cgcggcacgg cacggcctac acgcggaccg gtcagtaccc
    20521 cgtgaaggcc ggactccata aggccgatac gcggccggtc gaagaggggt gcgactgtta
    20581 ttgttgccgg accttttcgc gggcctacgt gcggcatctg ctgaacgtgg gggaaatttt
    20641 gggggtgcgg ctgctgacga tccacaacat gcaccgttac ctcgggttca tgcgggagat
    20701 gcgcgaggcg atcgccaacg acgcattcgg tgaatggcgg cgcgcgctcg ccgccgtgtt
    20761 gcggccggac ggccggaaac ccgtccggat gggcgcccgg gcgccgcagg cagggtgagg
    20821 gaaagcgcgc gcggcacagg tggtggcgcg cgggtttgga gtgagcgaga aaggatacgt
    20881 gatgacgaca gggtggtttt gggcgcaggc ggcgcagcag ggtacgggcg gggttggcgg
    20941 gatgctgatc cccatggttc tggtgttcgg tcttttctat ttcatgatga tccggccgca
    21001 acagcgtaag gagaaggagc ggttgcgcat gatcgccgaa ttgcgcgccg gagagcgggt
    21061 gatgttcgcg gggggtctga tcggcaccat cagcgaggcg cgcgaaacca cgtttgtggt
    21121 tgagatcgcc cccaaggtga tggttgaggt cgcgcgcggc gcggtcacgc gggtgttgaa
    21181 agagggggat gtcgccactc tggaagaggg caggtgagcc gttttttcat ttcttgggtg
    21241 aaatggccac ggctttttga taatttaaat ctttctcctg accctttccg ggttccgggt
    21301 aaattgtgga gttgctgaaa tgagtagaaa cgatctctgg aaatggctga tactggcttt
    21361 tctaactgtt ttgtctgcct atgttgtgat tccgccgaag gagaagatcc ggttggggct
    21421 ggatctctcg ggcggcacga gtttcacggt ggcgatcgat gaggcgaaac tgcgcgccaa
    21481 tgtaagggcg gccgcggaca cggaactcac cgacgtggag gtcgagaatc aggtgaacgc
    21541 tgtgctcagg gatgcggatg cgcgggccat tgaggtgatc cgtaaccgca tcgacgggct
    21601 gggcgtcaat gagccggtca ttcagggagg caagaatcac cgcattgtgg tgcagcttcc
    21661 gggggccagt gccgagcagc gggatgcc
//
LOCUS       DAOSLE010000028        21575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2690_length_21575_cov_7.616217, whole genome shotgun sequence.
ACCESSION   DAOSLE010000028 DAOSLE010000000
VERSION     DAOSLE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21575
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2690_length_21575_cov_7.616217"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK576_03505"
     CDS             <1..221
                     /locus_tag="PK576_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881273.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOE60687.1"
                     /translation="VQAAIGRTIIAREDIRAFRKDVLAKLYGGDVTRKQKVLKKQKAG
                     KARMKEFGQVNVPQSAFISVLKGSSKDD"
     gene            254..1417
                     /gene="lepB"
                     /locus_tag="PK576_03510"
     CDS             254..1417
                     /gene="lepB"
                     /locus_tag="PK576_03510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE60688.1"
                     /translation="MNYFEKRKIRKQLKTVLHHARTLRCSREDIMRADDLASLNEHIA
                     QAQEAYAARAGEAMKTTGNALETCVTRLNPPRPLSGWRENFDVLVVAISVAMAFRAYF
                     YQPFKIPTGSMQPTLYGIHSESQPPSAATLLDRQPLKFVKWLVTGESFKTVRAKASGT
                     VHFSSSDSSKKPGYMPVIVAGIPHYVPNDAVEVDAYRRPIRLKGGVANGASVRAGEVL
                     WSGVVVSGDFVFVNRWKWNIAHPQRGEVMVFSTIGITALPQGTHYIKRMTGLPNETLQ
                     IKAPNLLVNGKAVTEPLRIGQIARQEKVWPNSPAYAGFNPSGQPQMVLRGRSLATEQD
                     QVTLGPDQYYAMGDNSFNSFDSRYWGPVPERNLLGPATVVYWPFTSPRFGWIR"
     gene            1504..2232
                     /locus_tag="PK576_03515"
     CDS             1504..2232
                     /locus_tag="PK576_03515"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOE60689.1"
                     /translation="MSANQMTRHQMGLSRRAFVTVAAGAAASLTAAQQGGAAAAAITL
                     PPLPFPSDALAPVVSAQTLSFHHGKHHQGYVNTLNKLLPGSKFAGLTLEAIVRQSAGL
                     AEHAAVFNNAAQIWNHTFYWKSLKPVAVAQTVPAPLAAAVTRCFGSLDACKQAMSDAA
                     LKQFGSGWAWLVAERGALKVVKTPNAETPLTQVGVTPLLTIDVWEHAYYLDWQNRRAD
                     YVKAVLDQALNWPFAAERFAAATA"
     gene            2277..3395
                     /gene="alr"
                     /locus_tag="PK576_03520"
     CDS             2277..3395
                     /gene="alr"
                     /locus_tag="PK576_03520"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOE60690.1"
                     /translation="MSELPRRVWLEISLETLTRNYRNIAAAVAPAGVLTVLKANAYGL
                     GVVPIAEALCRAGAAGFGVAEPHEALQLLSLGKPVQILSSVLPEEIEPMVEAGVTLPV
                     TDLETARRISAAAAACGRTATVHIKLDTGMGRLGVLAHEAPDVIRAVHALPGLACEGI
                     FSHFPFAYETGSELTNRQMDRFIEIIEELAREGITFDQRHIANSDAINNFPRACRAPF
                     NVVRTGINLHGSFDTVGRRALHLAPVLTLKTRLTAIRTLPAGTGIGYGHTFRLHRSTR
                     VGTVSAGYADGMPLALSNRGYLLIRGTPCPILGRLSMDYTTVSLESVPDARIGDEVVC
                     LGGEGMHALTVEDWATLKNTHPYEIICSFGNRVERRYV"
     gene            complement(3386..4609)
                     /locus_tag="PK576_03525"
     CDS             complement(3386..4609)
                     /locus_tag="PK576_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE60691.1"
                     /translation="MNSRREAIFILTRWLATHDFPDRLLPDGPDRAFITDLVYTTVRR
                     RRTLEWVLQQLMRRLPQGETGAALLAGACQILFMPDVAEYAAVNETVEAAKLASKQTA
                     GLVNAVLRNLLRQRETLLARLAKESVGIRTSHPDALITRWRERFGAEETLALCEWNNT
                     PAETFLAYPPGADEPFVPMPRGARVTEMPGFEEGAFIVQDPATAVSVDLLAVRPGLSV
                     LDACAAPGGKTVQIAWRMGVPGPDPRHRLVALDLHEDRLRLVRENLARTRQEWVTVCS
                     GDLTQNPSSALSEFGLFDRILLDVPCSNTGVLRRRPDARWRWTTPRMKSLAATQAVML
                     ENALTLLAPGGRLVYSTCSLEPEENRRQITVLRKRHPEIACIGVEERIPTRHATDGVF
                     ACALERRHDVRGTQT"
     gene            complement(4764..6698)
                     /locus_tag="PK576_03530"
     CDS             complement(4764..6698)
                     /locus_tag="PK576_03530"
                     /inference="COORDINATES: protein motif:HMM:NF027658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOE60692.1"
                     /translation="MKAIERALFCGLVAAISIDAMAAFRPPAVPLVSVEPHFSLWSAA
                     DRLYERDTTHWAGATQSLVILLEADGITYRLCGHGPRDLVELPVLPQTRCRVGATTTY
                     YRFADGRGLSAEVAFTTPKLTDDLDVFSRPVTYLNVRVAGAKTFAVRTSITGAFATND
                     DRAPMTWTTNGVAGATVVAVGRADGTPFSIRGDRQRANWGRACLVGPIADGDTTRYLL
                     AYDNVKSVRFFGQELVDWWRRVGKTFDAMLGEAVRDASALEAKCRAFDVRFRADMEAV
                     GGAKYADIAELAWRQSFAACKFVAGPDKEPFFLSAENASGGMIGTTDVFYPQFPHLLM
                     SSLTLAKASLAPTCIYAASTNWPYAYAPHDLGLFPVAEGQYYGMKKGQSVGGGTDDTY
                     RMPVEECGNMLILLGAVATAEGNAGFAGRWWDEVTKWADYLAAFGFDPGNQLCTDDFA
                     GHLAHNANLSMKSIVALAAYARMAEMRGETDAAHRFRSLAEGMVPQWRAAAKGGAAGG
                     SRIAFDRPGTWSQKYNLVWDSVLGFNLFPAEVAAAELAAYRAVALPYGVPLDCRKTYT
                     KADWIVWSGTLTGRREDLEFMCEGLHRFLNETPDRIAFGDWYLTDSALYRHFIARSVV
                     GGVFMPVLRHADLVKRYRHP"
     gene            6789..8297
                     /gene="der"
                     /locus_tag="PK576_03535"
     CDS             6789..8297
                     /gene="der"
                     /locus_tag="PK576_03535"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOE60693.1"
                     /translation="MGSVKRERGVDGPRAMLALVWARGYGYTQRMISQDQGKARVVVI
                     VGRPNVGKSAVFNRIAGRRIAIVHDESGVTRDRLMREVEWDDQRFTLIDTGGITLLTG
                     QTGKNAIEAGIRAQVDAAIGDAAVALLVVDVKQGMHPLDEEVALIVRKAGIPCVVAVN
                     KCDEPQHEAGVAEFARLGFATFPVAAQHNRGMGELMEAVLPYLPEAVNPTETQPLRVA
                     VVGRPNAGKSSYINRLLRSDRVIVSDVAGTTRDSIDVPFTIGSGAQARHYILVDTAGM
                     RNRHRIDNAVERFSLFRAEESIRGADVVVLMLDAEIGPTVQDKHIAAMIQKYNKGCVL
                     LMNKWDRAMENGMTQTRSEPVLRQIMPFMGYSPVLFISAVTGFNVRKSVETIDAVAAQ
                     TRVTLPTGILNRTLVEATERVLAPARNGKRLRVYYAVQIGVAPVSIRLFVNDPKLATK
                     PYTDFLIRSLRSRFGLEGAPVRLFYRARTRPESVERPARSRSRARGKKRKAR"
     gene            8350..9714
                     /locus_tag="PK576_03540"
     CDS             8350..9714
                     /locus_tag="PK576_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOE60694.1"
                     /translation="MAGEGLSVKTGRALVLCALTGLVAGLAASGFYWLLEAGRYLLLE
                     RLGHYSPPLAAGEFPLFPPVAHEAPVRWVLLLLPAVGGLLSALLVKGFAPSAGGHGTD
                     AAIYAYHRNEGRIPFAVIPVKALASALVIGSGGSAGCEGPVTQIGAGCGSAVAGLLKL
                     DATERRILMAAGLAAGVGAIFRAPLAGAIFAAEIFYSGLDIEYEVMMPSLVASTIAYT
                     VFALFFGWQPLFVMTPYNFDAPLKLGPYLLLALVVAFGAKLYIHVFRRTEAWCRNLSR
                     VPTWLKPALGGMLTGLIGFFLPEVLGSGYGLIQQALAVGSPLAERFGSPSFTMLWLIF
                     FGKILATSFTVGSGGSGGVFGPALVAGSTLGAATGLTLMRLLPALDLHPGAFALVGMA
                     GFLASCVRVPIAAIIMVSEITGNHELLLPSMWVCGIAYWIGNGWTLYRSQVRTRDCSP
                     AHSR"
     gene            9767..10453
                     /gene="tsaB"
                     /locus_tag="PK576_03545"
     CDS             9767..10453
                     /gene="tsaB"
                     /locus_tag="PK576_03545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE60695.1"
                     /translation="MRILVIDRSTSRAGMALMDAGRAICSRVWEDDAARSPGWIAEAV
                     RELAACGVGLQEVGRFVCGLGPGSFSGIRSSLAALQGMALPTGAPVCGVASAAALALA
                     YGMDVPRVTVVGDARRERLWIVTYRVEPHTGRVMLADGTRPTHTAADFTLVPASELAS
                     AIPGDAFVVSSDWARLAPRLTAACPSPRLLRETVCPDAADLGRLACADLDACVTEPLP
                     IYLHPAVVVR"
     gene            complement(10763..11971)
                     /gene="dinB"
                     /locus_tag="PK576_03550"
     CDS             complement(10763..11971)
                     /gene="dinB"
                     /locus_tag="PK576_03550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE60696.1"
                     /translation="MQRCILHVDMDAFFAAVEQRDHPEWRGKPVIVGAPPDRRGVVST
                     CSYEARVFGVRSAMPSREAYRRCPHAIFVPCDMGRYEEASQQVFAIFERFTPLIEPLS
                     IDEAFLDVTGGRTLFGSGEKIARAIREAIRNEVGLTASVGIAHNKFLAKLASEQAKPD
                     GLAVIPETTDAILAFLAPLPVRALWGVGRVTAQTLEKGGLRRIADIRQADPAFLASLV
                     GEHAATHLMALAHGEDARPVQTDFEEKSISREHTFPEDCRDRSVVCEVLKTLADDVGR
                     RIRLQNRYATLGKLKLRWADFRTLTRQAPFPSPVCDDFAFREQALRLFQAQPLTQPVR
                     LIGFGVSGLCNECGAEQLALFDSVPDAREKRERLCRTLDQVRERLGDHALRRVSVPEN
                     EVEKGRAGSP"
     gene            complement(12054..12620)
                     /gene="infC"
                     /locus_tag="PK576_03555"
     CDS             complement(12054..12620)
                     /gene="infC"
                     /locus_tag="PK576_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006723876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE60697.1"
                     /translation="MNFKIRVPNVRVINANGEMLGIMATRDAQRLADQQGLDLVEVSP
                     NAEPPVCKIMDYGKFRYEESIKRKMARKNQKTVQIKEVKFHASVDSNDLAHKLRQIKE
                     FLNDGHKVKVTLQYRGRENAHKELGMEVVQKVIQECAACSVVEQNPRLIGRTLGCLLA
                     AKPAKGAASGQSAASAPQNPPPRPPAAP"
     gene            complement(12747..14492)
                     /gene="thrS"
                     /locus_tag="PK576_03560"
     CDS             complement(12747..14492)
                     /gene="thrS"
                     /locus_tag="PK576_03560"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOE60698.1"
                     /translation="MEVGVEAIDVMRHSAAHVMAHAVCNLFDNVKLDIGPSTEEGFYY
                     DFDLEHRLSPEDFPRIEAEIEALIQADLPFERLETTCGEAARILADQPYKLERLADIP
                     EGEAITLYRCGAFVDLCRGPHVNRTSEIGAVKLTSVAGSYYRGDEKNAMLQRLYGMAD
                     VSREALDAVLARMEEAKKRDHRKLGKELDLFSISEEVGPGLAHWHPKGARIRVAIEDY
                     WRREHFRAGYEMVFTPHVGRAKLWETSGHLGFYRDSMFPSMNLSEGEGSRASVESYFV
                     KPMNCPFHIQVYKTRRYSYRDLPLRWAELGTVYRYEKEGVRHGLLRVRGFTQDDAHIF
                     CTPEQIEDEVRSTVTFALRVLRRFGFREITAFLSTRPEKAVGLPERWEQATQSLENAL
                     RAEAIEYQVDAGGGAFYGPKIDLKIKDALGRPWQLGTIQFDFNLPERFALTYIGEDGK
                     EHQPYMVHRALLGSLERFFGILIEHYAGAFPVWLAPEQVRVLPITADQIAYARSLETT
                     LREADVRVTVDTDNEKLGAKIRRAQLEKVPFMLVVGKRDAEAQVVSVRSRETGDQGAM
                     TIEAFRSVLADAMNA"
     gene            14836..18246
                     /locus_tag="PK576_03565"
     CDS             14836..18246
                     /locus_tag="PK576_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE60699.1"
                     /translation="MRGRLGLAWVLAGSLALAEGVVESAKTLPLAHETDVVIVGGSSG
                     AVAAACRAAAEGVRVFVVAPRPYLGEDMAGKLCLRTGASEDPRCVLRRRMFTTTQLPP
                     SLPFSYRSDRAAASNHPDPHHRVLSDGVWQDAVTESAQFGGDVTLTLDLGALTDLGSL
                     VACVFRRDGANGFRTGALHVWGSTDAETWADLGKGIDQETEAGSERARFRVPLSGRYR
                     YVKVLALVAPGVKRQLLGELLVYPVADPSAGAGLSDRTTPLQVKKVLDEALLQAGVPF
                     LTGALATETLTDETGALAGVVIANRSGRQAIRAKVVIDATERGILARAAGGQAEPFPA
                     GHYTFTRRIVAGEAPRAEGVRVRELFGVYPVRVTGIRPPEGHPGTITGRLFECEIDLP
                     MRDGSARAFAEAEQRARDLTFVPTQLESADTLSLLPPDFIRGKAAVRAPWAGVDALDL
                     DAFQPAGTSFLFVLGPMADVARPTVEHLMRPGNLMALGERIGAAAARLAKQRPAPQGV
                     RAGGSPDAQSGGVTVGEHTRNLAAYLRNASGTVRVEPRELPVLAECDVLVAGAGTGGA
                     PAGIAAARHGARTLVCEYIHQMGGVQTDGLIGRYYFGNRVGFTLDIDRGVKETGAVFS
                     QCKSEWYRSQNRKGGAEIWYGTMVSGVVVENNRLTGVVVVMPDGTRGVVRCRVAIDST
                     GNAELAAMAGEKTEFITAAEVALQGVGQTPRRMGASYTNTDIGFLDDTDASDLCFFAL
                     RSRYSMREGIWDQAQIINSRERRRMIGAFYMSPLDVVNGRTYPDVVVQPYSNFDSHGH
                     TTHEQFFIEDPGHHGMKVNLPFRCLLPKTVDGLLVTGLGISAHRDAMPILRMQPDVQN
                     QGYAAGTAAAMAIKAGVPVRRVDIRALQRHLIEMKILPETVLDMEDSFPLPDACFTEA
                     VAAIPNGYQGLSVVLTDPARSVPLLKAAYTAARVPEARLAYAHVLGMMGHPDGETELI
                     ARVQGMAWDKGWHFRGMGQFNRSVSWLDSYLIALGRCRSARAVPAILDKAKALTAADA
                     FSHFRAVALALEAIGDPAGAPVLGRLLATEGIGGYAIRMGPELPVIPNYANHEGDRER
                     SACLRELALARALFRLGDFEGRGVRTLRAYAEDPRGAYATHAALVLGR"
     gene            18386..21481
                     /gene="secA"
                     /locus_tag="PK576_03570"
     CDS             18386..21481
                     /gene="secA"
                     /locus_tag="PK576_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE60700.1"
                     /translation="MNLIRTLFGTKNERDIKRLQPMVTRINELEKEYQALSEQQLQAK
                     TQEFKERVAAGESLDDLLCEAFAVVKNACRRLCGTTREVCGHMLAWDMVPFDVQLIGG
                     IVLHQGKIAEMQTGEGKTLVATLPLYLNALSGNNVRLVTVNDYLARRDAQWMGHVYEY
                     LGLTVGCIQNHMGPAERREQYACDITYGTNSEFGFDYLRDNGMAVEPSQVVQRGHHFA
                     IIDEVDSILIDEARTPLIISGPVAHVSTAQYTELRPRVERLVRDQMEICNRLISEVRE
                     AIEAADSETAMFKLYQVYHGMPKHKQFLHLLEDPATRKLHEQVESMMLSEMRKELARD
                     LREQLLFTIDERTREVALTDRGSEKLSPTDPEMFVMPDLVSAMSALDGDTALGAEERA
                     KRRQQIQSDFMVRSERVHNVDQLLRAYCLYERDVDYVVQDNHVYIVDEFTGRVLPGRR
                     WSDGLHQAVEAKEGVEIERETQTLATITIQNYFRLYKKLAGMTGTAETEASEFHDIYK
                     LDVVVIPTNRPIRRVDGNDQIYKSQREKYKAILDEVEKRHRNGQPVLLGTVTVDTSEI
                     LSRMLRMAKIPHNVLNAKNHAREAEIVSLAGQPGAVTIATNMAGRGTDIKLGEGVVWV
                     PETTIRSQITLENRYDNGNKTLRKLLEEKPCGLHVIGSERHESRRIDRQLRGRCARQG
                     DPGSSQFYISLEDNLMRLFGSDRISGIMTRLGMQEGEALEHKWLNRSVETAQRRVEQQ
                     NFAIRKRTLEYDDVMNKQRSVVYDLRGDVLTAETAHNHILDVFNDLILTQSERYLSSA
                     KDAMPQELVRWVTDTFPVAVREEEIAAFRGEPEKAGELLYTRVAEAYELKCSMEDPAV
                     LPIMERSVFLSCIDQQWQSYLRAMDELRHGVNLRAYGQRDPLVEYKREAFTMFEELMT
                     NIKTDVATSVFRATTMQNFQRMMAGPGGRVQMVHNDINILAGGETPQPAAGQAGRGLT
                     AADVPQAAVAGFDAILDAMDRTGQSAPHATTGPLIAKPSVGRNDPCPCGSGKKYKKCC
                     G"
BASE COUNT         3842 a         6306 c         7294 g         4133 t
ORIGIN      
        1 cggtgcaggc cgcgatcggg cgcacgatca ttgcccgcga ggatatccgc gcgttccgca
       61 aggatgtctt ggccaagcta tacggcggcg acgtgacgcg caagcagaag gtgttgaaga
      121 aacagaaggc cggtaaggcg cgtatgaaag agttcgggca ggtcaatgtg ccgcagtccg
      181 cctttatctc cgtgcttaag gggtcatcaa aggacgattg atcgtaaagg cggcggaaga
      241 ggggagtcca agcgtgaatt attttgagaa gcggaagatc agaaagcagc tcaagaccgt
      301 gctgcatcac gcgcgcacgt tgcggtgcag tcgtgaggac atcatgcgtg ccgacgatct
      361 ggcgtcgctg aatgagcata tcgctcaggc gcaggaggcg tacgccgcgc gagcaggcga
      421 ggcgatgaag actacgggca atgcgcttga aacgtgcgtc acgcgcctca acccgcccag
      481 gccgctgtca ggctggcgcg aaaatttcga cgtgttggtg gtggcgattt cggtggcgat
      541 ggccttccgc gcctatttct atcagccctt caaaatccct acgggctcga tgcagccgac
      601 gctgtacggc attcattcgg aatcgcagcc gccctcggct gccacgcttc tggatcggca
      661 gccgctcaag ttcgtgaagt ggcttgtgac cggagaatcg ttcaagacgg ttcgcgccaa
      721 ggcctccgga acggtgcatt tctcctctag cgacagcagc aagaaacccg ggtacatgcc
      781 ggtcatcgtg gccgggatcc cccattatgt gccgaacgat gcggtggagg tggatgccta
      841 ccgccgtccg atccggctca agggcggggt tgccaacggg gccagcgtcc gtgcgggtga
      901 ggttctgtgg tccggcgtgg tcgtgtccgg cgactttgtt tttgtcaacc ggtggaagtg
      961 gaatatcgcg cacccgcagc gtggcgaggt gatggtgttt tccacgatcg gcatcactgc
     1021 cctgccgcag gggacccatt acatcaagcg gatgaccgga ttgcccaatg agacgcttca
     1081 gatcaaggcg cccaatctgc tggtcaacgg caaggcggtg accgaaccgc tgcgcatcgg
     1141 tcagatcgcc cggcaggaga aggtgtggcc gaacagtccg gcctatgcgg ggttcaatcc
     1201 cagcggacag ccccagatgg tcctccgcgg cagatcgctg gctacggagc aggatcaggt
     1261 gacactgggt ccggatcagt attacgccat gggggataat tcgttcaaca gtttcgacag
     1321 ccggtattgg gggcctgtgc ctgagcgcaa tcttctcgga cccgcgaccg tggtgtactg
     1381 gccgttcacc tcgccgcgtt tcggctggat ccgctgaagc gttgcggagt ctgtgcaaag
     1441 aggttacact ggatccatct tttggcgaat gctacacagg ggtgaacccg tgaaaggagc
     1501 gcgatgagtg cgaatcaaat gacgcgacac cagatggggc tttcccgcag agcgtttgta
     1561 accgtggccg ccggggctgc ggccagcctg accgcggcgc agcagggggg tgccgccgca
     1621 gcggcgatca ccctcccgcc gctgccgttc ccttcggacg ctctggcacc ggtggtttcg
     1681 gcccaaacgc tttcgtttca ccacggcaag catcatcagg ggtacgtgaa caccctgaac
     1741 aaactgctgc cgggttccaa attcgccggg ctgacgctgg aggccatcgt gcgtcaatcg
     1801 gctggtttgg cggagcacgc cgcagtgttc aacaacgcgg cccagatctg gaaccatacg
     1861 ttctactgga agtcgctcaa gccggttgcg gtggcacaga cggtgcccgc tcctctggcg
     1921 gctgcggtca cccgctgctt tggttcgctc gatgcctgca aacaggcgat gtcggatgcg
     1981 gccctgaagc agttcggcag cgggtgggcc tggctggtgg cggaacgggg ggcattgaag
     2041 gtggttaaga cgccgaacgc ggagaccccg ctgacgcagg tcggcgtgac gccgctgctg
     2101 acgatcgatg tttgggagca cgcctattat ctcgattggc agaatcgccg ggcggattac
     2161 gtcaaggctg tgctcgatca ggcgttgaac tggccgttcg ccgcggagcg tttcgcggcc
     2221 gcgaccgcat gagggttgcc gcgcgcgtgg tgagagcaag tcggtaaggg ccggagatga
     2281 gtgagttgcc ccgcagagtc tggctggaga tcagcctcga aacgctgacg cgtaattacc
     2341 ggaacatcgc ggcggcggtc gcgcctgccg gggtgttgac ggtactcaag gccaacgcct
     2401 atggccttgg ggtagtcccg atcgccgagg cgctctgccg cgccggtgcg gcggggttcg
     2461 gcgtggccga accccacgag gcgctgcaac tgctttcgtt gggcaaaccg gttcaaattc
     2521 tgagcagcgt gctgcccgaa gagatcgaac ccatggtaga ggccggggtc acactgccgg
     2581 tcacggatct cgaaaccgcg cggcggatca gcgccgctgc tgcggcgtgc ggccggaccg
     2641 ccacggtgca catcaagctc gacaccggta tggggaggct gggggttctg gcgcacgagg
     2701 ctccggacgt catccgtgct gttcatgcgc tgcccgggtt ggcgtgcgag gggatctttt
     2761 cgcactttcc cttcgcgtac gaaaccggat cggaactgac caaccggcag atggatcgtt
     2821 ttatcgaaat catcgaggaa ctcgcgcggg aagggatcac ctttgaccag cgtcatatcg
     2881 ccaacagcga tgcgatcaac aacttcccgc gcgcctgccg tgcgccgttt aatgtcgtgc
     2941 gcaccggcat caacctgcat ggatcgttcg acaccgtcgg acggcgtgcc ctgcatctcg
     3001 cgcccgtgct gacgctgaaa acgcggctca ccgcgatccg gacgctgccc gccggtacgg
     3061 gtatcgggta cggccacacc ttccgcctgc accgttcaac ccgtgtgggc acggtctccg
     3121 caggttatgc ggacggcatg ccgctggccc tgtccaaccg cgggtacctg ctgatccgcg
     3181 ggacgccctg tccgattctg ggaaggctct ccatggacta caccaccgtc tcactggaaa
     3241 gtgtccccga cgccaggatt ggcgatgagg tggtctgtct gggcggcgaa ggcatgcatg
     3301 ccctgacggt cgaggattgg gccacgctca aaaacacgca cccgtacgag atcatctgtt
     3361 ccttcggcaa ccgcgtcgaa cggcgttacg tttgagtccc gcgcacgtca tgccgacgtt
     3421 cgagggcgca ggcaaagacg ccgtctgtgg cgtggcgggt cgggatgcgt tcctccacgc
     3481 cgatgcaggc gatttccgga tggcgtttcc gcaggaccgt gatttgacgg cggttctcct
     3541 ccggctccaa gctgcatgtt gaatagacga gcctgcctcc gggcgcgagt agcgtcaggg
     3601 cgttttccag catgaccgcc tgtgtggcgg ccagactttt catgcgcggg gtggtccagc
     3661 gccagcgggc gtcaggtcgg cggcgcagca caccggtgtt cgagcagggc acgtcgagca
     3721 ggatgcggtc gaacaagccg aactccgaca gggcggagga cgggttttgc gtcaggtcgc
     3781 ctgagcacac cgtgacccat tcctgacggg tgcgggccaa attttcgcga accagccgga
     3841 gccggtcctc atgcagatcg agcgccacga gccggtgcct ggggtccggg ccgggcacgc
     3901 ccatgcgcca ggcgatctgc acggtcttgc ctccgggagc cgcgcaggcg tccagcacgc
     3961 tgagacccgg acggacagcc agcagatcga cgctgaccgc tgtggccgga tcctgcacaa
     4021 tgaacgcgcc ctcttcaaat cccggcatct ctgtgacacg cgcgccgcgc ggcatgggca
     4081 cgaacggttc gtccgcgccg gggggatagg cgagaaaggt ttcggcaggg gtgttgttcc
     4141 attcgcagag cgcgagcgtc tcttctgcgc cgaaccgttc gcgccagcgg gtgatcaggg
     4201 catcgggatg ggaggtgcgg atgccgaccg actctttggc caaccgggcc aacaaggttt
     4261 cgcgctggcg caacaggttg cgcagcaccg cattgacaag gcctgcggtt tgctttgaag
     4321 cgagttttgc ggcctccacc gtttcgttga ccgccgcgta ttcggcgaca tcgggcatga
     4381 agaggatttg gcacgcgccc gccaacaggg ctgctccggt ttcgccttgg ggcaggcgcc
     4441 gcatgagttg ctggagtacc cactccagtg tgcggcggcg tcggactgtg gtgtaaacga
     4501 ggtcggtgat gaaagcgcgg tcgggtccgt ctggaaggag tcgatcagga aagtcgtgcg
     4561 tggcaagcca acgggtaagg atgaagatcg cttcgcgcct ggagttcatg cggtgtgttt
     4621 ttctgtggga tcggttatgg tcatcttccg gcacgttgct ccgggtaaga tgaagaacta
     4681 accttatatc cgcccaagcg gtttgtcgat ctgaaaacgt tatggtcaag tctcaacgtc
     4741 attcctgccg ctgattcgaa cccttacgga tgacgatagc gtttcaccag atcagcatgg
     4801 cgcagaaccg gcatgaacac gccgccgacg acgctgcggg caatgaaatg tcggtaaagg
     4861 gctgaatccg tgagatacca gtcgccgaac gcgatacggt cgggcgtctc gttcaggaac
     4921 cggtgcaggc cttcgcacat aaactcgagg tcttcgcggc gccccgtgag cgtgcccgac
     4981 cagacaatcc aatccgcctt cgtgtacgtc ttgcggcagt cgagcggcac gccataggga
     5041 agcgcgacgg cacggtaggc cgcgagttcc gctgcggcga cttccgccgg aaagagattg
     5101 aatccgagca cgctgtccca cacgaggtta tatttctggc tccaggttcc gggacggtcg
     5161 aaggcgatgc gtgagcctcc agccgctccg cctttcgctg cggcgcgcca ctgcggcacc
     5221 atgccttcgg cgagagaacg gaagcggtgc gccgcgtccg tctcgccacg catctctgcc
     5281 atgcgcgcat atgccgcgag cgcgacgatg gacttcatag agaggttcgc gttgtgcgcg
     5341 agatgccccg cgaaatcgtc tgtgcaaagc tgattgccgg gatcgaagcc gaaggcggca
     5401 agatagtctg cccacttcgt gacctcgtcc caccagcgtc ccgcgaaacc ggcgttgccc
     5461 tcagccgttg ccacggctcc gagcagaatc agcatattgc cgcactcctc gaccggcatg
     5521 cggtaggtgt cgtccgtgcc gccgccgacg gactgcccct tcttcatgcc gtagtattga
     5581 ccttcagcga ccgggaacag tccaagatcg tgcggggcat aggcgtatgg ccagttcgtc
     5641 gacgccgcat agatgcatgt cggcgcgagc gaggctttcg cgagcgtcaa actcgacatc
     5701 agaagatgtg gaaactgcgg atagaagaca tccgtcgtcc cgatcatgcc gccggaagca
     5761 ttctcggcgg aaaggaagaa cggctccttg tcggggcccg cgacgaattt gcacgcggcg
     5821 aaactttggc gccacgcgag ttcggcgatg tccgcgtact tcgcgccgcc gaccgcttcc
     5881 atgtccgcgc ggaaccgcac gtcgaaggcg cggcacttcg cctctagcgc ggacgcgtca
     5941 cgcacagcct cgccgagcat tgcgtcaaag gtcttcccga cgcgccgcca ccagtcgacg
     6001 agttcctgcc cgaagaaccg gacgctcttc acgttgtcgt aagccagcag gtagcgcgtc
     6061 gtatcaccgt cggcgatcgg ccccacgagg caggcgcgtc cccagttcgc gcgctgacgg
     6121 tcgccgcgga tcgagaatgg cgtcccgtcg gcacggccga cggcaacgac cgtcgccccg
     6181 gcgaccccat tcgttgtcca tgtcattggc gcacggtcgt cgttcgtcgc gaaggcgccg
     6241 gtaatcgacg tcctgaccgc aaacgtcttc gcgcccgcga cgcggacgtt gagataggtg
     6301 acggggcgcg agaagacgtc cagatcatcc gtcagcttcg gtgttgtaaa agcgacctcc
     6361 gccgagaggc ctcgcccgtc ggcgaaacga tagtacgtcg ttgttgcgcc gacgcggcaa
     6421 cgggtctgcg gcagcaccgg cagttcgacg agatcccggg gaccgtgccc acagaggcgg
     6481 tacgtgatgc cgtccgcctc caacagaatg acaagcgatt gcgttgcgcc cgcccaatgt
     6541 gtcgtgtcgc gttcgtaaag ccgatctgcc gcactccaga gactaaaatg aggctcgacc
     6601 gagacgagcg gcaccgctgg tggacgaaac gccgccattg cgtcaattga aatcgcggcg
     6661 acaaggccgc agaacaatgc tctttcaatc gctttcatct ttctgtccct tctttttcgg
     6721 tttggacgat gtatagaggg tttggggggt tgttgcaagg gtattgttga gaccgtcaag
     6781 aggcttcaat ggggagtgta aaacgggaac gcggggtgga cggcccccgc gccatgcttg
     6841 cgcttgtttg ggcaagggga tacgggtata ctcaacgcat gatttcacag gatcagggca
     6901 aggcaagggt tgtcgtgatt gtcggacgcc ccaatgtcgg caagtcggcc gtattcaacc
     6961 gcattgcggg caggcgtatc gcgatcgtgc atgacgagag cggggtgacc cgcgaccggt
     7021 tgatgcggga ggtggagtgg gacgaccagc gtttcaccct gattgacacg ggcgggatca
     7081 ccctcctgac cggccagacg ggaaagaacg cgatcgaggc ggggatccgc gctcaggtcg
     7141 atgcagccat cggggatgcc gcggtggccc tgctggtcgt ggatgtgaaa caggggatgc
     7201 atccgttgga cgaggaggtc gcgctcatcg tgcgcaaggc cgggattccc tgtgtcgtgg
     7261 ccgtcaacaa gtgcgatgag ccgcagcacg aggcgggagt cgctgaattc gcgcgtttgg
     7321 gttttgcgac ctttccggtt gcggcgcagc ataaccgggg gatgggggaa ctcatggagg
     7381 cggtgctgcc gtacctgccg gaggcggtca acccgacgga aacccagccg ctgcgcgtgg
     7441 cggttgtagg gcggccgaac gcgggcaagt cctcgtacat caaccggttg ttgcgcagtg
     7501 accgcgtgat cgtttcggat gtcgcgggca cgacgcgtga ctccatcgat gtgccgttca
     7561 cgatcggctc cggcgcacag gcgcgacact atattctcgt ggacacggcg ggcatgcgca
     7621 accggcaccg cattgacaat gcggtggagc gcttcagctt attccgtgcg gaagagagta
     7681 tccgtggagc cgatgtggtg gtgctgatgc tggatgccga gatcggcccc accgttcagg
     7741 ataagcatat cgcggcgatg attcagaagt ataacaaggg gtgtgtgctg ctgatgaaca
     7801 agtgggatcg cgcgatggag aacgggatga cccagacccg gagcgaaccg gtgttgcgtc
     7861 agatcatgcc cttcatgggg tatagcccgg tgttgttcat ctcggcggtt accgggttca
     7921 acgtgcgtaa aagcgtggag accatcgatg ccgtggcggc tcaaacacgg gtgaccctgc
     7981 cgaccggcat cctcaaccgc acactggtcg aggcgaccga gcgtgtgttg gcgcctgccc
     8041 gcaacggcaa gcgcctgcgg gtctactatg ccgtgcagat cggagtcgcg ccggtttcca
     8101 tccgtctgtt tgtcaatgac cccaaactcg ccaccaaacc gtacacggat ttcctgattc
     8161 gctccctgcg ttcgcgtttc ggtttggagg gggcgcccgt gcggttgttc taccgcgcgc
     8221 ggacgcggcc cgagtcggtg gaacgccccg cgagaagtcg cagccgcgcg cgcgggaaaa
     8281 aacggaaagc ccgatgaggg ggcggtaggt ttgtttcgta aaatttctat acagtggtgg
     8341 cggttttgga tggcgggcga gggattgtcc gtcaagaccg gtcgtgcgct ggtgctttgt
     8401 gcattgacgg gactggtcgc cggtttggcg gcatccggat tctactggct gctggaggcg
     8461 gggcggtatc tgctgttgga acggctgggg cactactcgc cgccgttggc cgctggcgag
     8521 ttcccgctct tcccgccggt ggcgcacgag gcaccggtgc gctgggtttt gctgttattg
     8581 ccggcggtcg gtggtctgct cagtgcgctg ttggtgaagg ggttcgcgcc ctcggcgggc
     8641 gggcacggca cggatgcggc gatctatgcg taccaccgca atgaggggcg gatcccgttt
     8701 gcggtgatcc cggtgaaggc gctcgcttcg gcactggtca tcgggtctgg cggctcggcc
     8761 ggttgcgagg gcccggtgac tcagatcggc gcggggtgcg gttccgcggt ggcggggctt
     8821 ctgaagttgg atgcgaccga gcggcgtatc ctgatggcgg cgggattggc ggcgggtgtg
     8881 ggcgcgattt tccgcgcgcc gctggcgggc gcgatctttg cggccgaaat tttttacagc
     8941 gggctcgaca tcgagtacga agtgatgatg ccctccctgg tcgcctccac gatcgcctac
     9001 accgttttcg cgctgttttt cggctggcag ccgctgtttg tgatgacgcc ctataatttt
     9061 gacgccccgc tcaaactggg gccctatctg ctcttggcgt tggtggtggc cttcggcgcg
     9121 aaactgtaca tccatgtgtt ccgcagaacc gaggcgtggt gccgtaatct gtcgcgggtg
     9181 ccaacgtggc tcaagccggc cctcggcggt atgctgaccg gcctgatcgg gttcttcctt
     9241 ccggaggtgc tgggctcggg gtacggtctg atccaacagg cgctggcggt cggcagtccg
     9301 ctggccgaac ggttcggctc tccctcgttt acgatgttat ggctgatctt tttcgggaaa
     9361 attctggcaa cctcgttcac ggtcggcagc ggcgggagcg gcggcgtgtt tggtcccgca
     9421 ctggttgccg gatcaacctt gggtgcggca acagggctga ccctgatgcg gttgctgcct
     9481 gcgttggatc tgcatcccgg cgcgttcgct ctggtgggca tggccggttt tctggcatcc
     9541 tgcgtgcgcg tgccgatcgc ggcgatcatc atggtcagcg agattacggg aaaccatgaa
     9601 ctgctgttgc cgagcatgtg ggtgtgcggc atcgcgtatt ggatcgggaa cgggtggacg
     9661 ctgtaccgca gtcaggtgag gacacgcgac tgttcgcccg cgcacagccg gtgagggacg
     9721 cggtgttcgg gtcccggtgc ggaacgtgga tagggagggg tggaggatgc gtattctggt
     9781 gatcgaccgt tccacatcca gagcaggcat ggcgctgatg gatgcgggca gagcgatttg
     9841 ttcgcgggtc tgggaggatg acgccgcgcg gtcgccgggt tggattgcgg aggcggtccg
     9901 ggagttggcc gcatgcgggg tgggactgca agaggtgggc cggtttgtgt gcgggttggg
     9961 accgggctcg ttttccggga tccgttccag cctcgcggcg ctgcagggga tggcgctgcc
    10021 gacaggtgcg cccgtctgcg gtgtggccag tgccgcggcg ctggcgctcg cgtacgggat
    10081 ggacgtgccg cgggtaacgg tggtgggcga cgcgcggcgg gaacggttgt ggattgtgac
    10141 atatcgggtg gaaccgcaca cggggcgggt gatgctggcg gacgggacgc gtccgaccca
    10201 cacggctgcg gatttcacgc tggtgccggc gtcggaattg gcttcggcga tacccggtga
    10261 cgcgtttgtg gtaagttcgg attgggcgcg tctcgcgccg cgtctcacgg ccgcatgtcc
    10321 ctcccccaga ttacttcgtg agaccgtctg tcccgatgcc gcggatctcg gccggctcgc
    10381 ctgcgccgat ctggacgcat gcgtcaccga gccgttgccg atctatctgc atcccgcagt
    10441 cgtggtgaga taatgtgatc cgcaggttac attgaaccta aaaaagggca gttgaacttt
    10501 caacgctctt cggtttcagg tttcatgcat ggctcgccct acctgttcac agcgtcagcg
    10561 gtacacgttc acgtacacgt ccgcgaaaca cgaacagggt gccgattccg acagcgatcc
    10621 cgatttcgac gccgttgctc tgcaagtccg gcaaatggat tttgcgtcag caagcggtgt
    10681 tccgcacgcc gccgctttct gatcaggagt gcggtggcag ggcaatgcaa tgcgcgacgc
    10741 gacggcatgg ctcggcggcg gtttacgggg aacccgccct gcctttttca acttcgttct
    10801 ccggcacact cacgcgtcgg agcgcgtggt cccccaaccg ttcgcgcacc tgatcgagtg
    10861 tacggcagag ccgttcgcgt ttttcgcgcg cgtccggaac ggaatcgaag agcgcgagtt
    10921 gttcggcgcc gcactcgtta cagaggccgc tgaccccgaa tccgatcagg cggacgggtt
    10981 gcgtgagcgg ttgagcctga aaaaggcgga gcgcctgttc gcggaaggcg aagtcatcgc
    11041 ataccgggct cgggaacggg gcctgacgtg tgagggtacg gaagtccgcc caacggagtt
    11101 tcagtttgcc gagggtggcg tagcggtttt ggaggcggat ccgccgcccc acatcatcgg
    11161 ccagtgtttt cagaacctcg caaacaacgg aacggtcgcg gcagtcctct ggaaaggtgt
    11221 gttcgcggga gatgcttttt tcctcgaaat cggtttgcac ggggcgcgcg tcctccccgt
    11281 gggccagggc catcaggtgc gttgccgcgt gttcgcccac aaggctcgcg agaaacgccg
    11341 ggtctgcctg ccggatgtcg gcgatacggc gcaagccacc cttttcgagc gtttgggcag
    11401 tgacgcgtcc gaccccccac agcgcacgga cgggtagggg ggcgaggaag gcgagaatcg
    11461 catcggtcgt ctcgggaatg acggcgaggc cgtccggctt ggcctgttca ctggccagtt
    11521 tcgcgaggaa cttgttgtgc gcgatcccga cggacgcggt gagtcccact tcgttgcgga
    11581 tcgcctcacg gatggcccgc gcgatcttct ccccgctgcc gaagagggtg cggccgccgg
    11641 tgacgtcgag aaaggcctcg tcaatggaca gcggttcgat cagcggggtg aagcgctcga
    11701 agatggcgaa cacctgctgt gaggcctcct cgtaccggcc catatcgcag gggacaaaga
    11761 tcgcatgcgg gcagcgccgg taggcctccc gcgaaggcat ggccgaacgc acaccgaaca
    11821 cgcgcgcctc atacgaacag gtggacacca ccccgcggcg gtccggaggc gcgccgacaa
    11881 tcaccggctt gccgcgccat tcggggtggt cacgctgttc gaccgccgcg aaaaaggcgt
    11941 ccatgtcaac gtgtaggatg caccgttgca tagccgactc cggtcagggc acaagaatac
    12001 ggccgggaac cgcgccgatc cgcctcacgg tctccggcaa caacagggtt cagtcaaggc
    12061 gcggcgggcg gacggggtgg cgggttctgc ggcgcactgg cagcggactg tccggatgca
    12121 gcgcccttcg ccggtttggc tgccagcaga catcccaacg tgcggccgat cagacgcggg
    12181 ttttgttcga ccaccgagca cgccgcacac tcttggatca ccttctgcac gacttccatg
    12241 cccagttctt tgtgcgcgtt ttcgcggccg cggtactgta gggtcacctt gaccttgtgg
    12301 ccatcgttga ggaactcttt gatttgacgc agcttgtggg cgaggtcatt cgaatccacg
    12361 ctggcgtgga atttcacctc tttgatctgc acggtcttct ggtttttgcg cgccatcttc
    12421 cgcttaatgc tttcctcgta gcggaacttg ccgtaatcca tgattttgca gaccggcggt
    12481 tcggcattcg gggacacctc gaccaagtcg agtccctgct ggtcagccag acgttgcgca
    12541 tcccgcgtcg ccatgatgcc gagcatctcg ccgttggcgt tgatcacccg cacgttgggg
    12601 acccggattt tgaagttcac tcgggtaaac gaagcgataa ctcacctccg atgttgagcc
    12661 gacttacggc tcgtttctgt tttgtttccg ctccggatga accggagccc gcatcctgtc
    12721 tttatccgcc tccgggtacg cgaaggtcac gcgttcatcg cgtcagcgag cacgctccgg
    12781 aaggcctcga tcgtcatcgc gccttgatct ccggtctcac ggctgcgcac cgaaaccact
    12841 tgggcttcgg catcgcgttt gccgaccacc agcataaaag gaactttttc gagttgggcg
    12901 cggcggatct tcgcgcccag cttctcgttg tcggtatcga ccgtcacgcg cacatccgct
    12961 tcgcgcagag tcgtttccaa cgagcgggcg tacgcgatct gatccgcggt gatgggtagc
    13021 acgcgcacct gctccggcgc cagccacacg ggaaacgcgc ccgcatagtg ttcgatcaga
    13081 atgccgaaaa agcgttccag gctgccgagc agggcgcggt gcaccatata gggctggtgc
    13141 tccttgccgt cctcgccgat gtatgtcagc gcgaaccgtt ccggcaggtt gaagtcgaac
    13201 tggatggtgc cgagttgcca cggccgtccg agcgcgtctt tgatcttgag gtcgatcttc
    13261 gggccgtaga acgcgccgcc gcccgcatcc acctgatatt cgatggcctc ggcccggagc
    13321 gcgttttcca aggattgcgt ggcctgttcc caccgttcag gcaacccgac cgccttctcg
    13381 ggccgggtgg agaggaaggc cgtgatctcc cggaacccga aacggcgcag aacgcgcagc
    13441 gcaaaggtca cggtggaccg gacctcatcc tcgatctgct ccggcgtgca gaaaatgtgc
    13501 gcatcgtcct gcgtgaaccc gcggacgcgc aacaacccgt ggcgcacgcc ctctttctca
    13561 tagcggtaca cggtgccgag ttcggcccat cgcaacggca ggtcacggta ggaatagcgc
    13621 cgggttttgt acacctggat atggaacggg cagttcatgg gcttgacgaa atacgattcg
    13681 accgaagccc gcgagccttc gccctccgac agattcatgc tcgggaacat gctgtcacgg
    13741 tagaacccca gatgccccga ggtttcccac agtttggcgc gccccacatg cggggtgaac
    13801 accatctcat atccggcgcg gaagtgttcg cgccgccaat agtcctcgat cgccacccgg
    13861 atgcgcgcgc ctttgggatg ccagtgcgcc aatcccggcc cgacctcctc gctgatacta
    13921 aacaggtcaa gctctttgcc gagtttgcgg tggtcgcgtt ttttggcctc ctccatccgg
    13981 gcgagcaccg cgtccagggc ctcgcgcgac acatccgcca tgccgtacag acgctgcagc
    14041 atggcgtttt tctcatcgcc gcggtagtag gatcctgcga cggacgtcaa cttgaccgcg
    14101 ccgatttccg aggtgcggtt cacatgcggt ccgcggcaca aatccacgaa cgcgccgcag
    14161 cggtaaaggg tgatcgcctc gccctcgggg atgtccgcga ggcgttccaa cttgtaaggt
    14221 tggtccgcca agatccgcgc cgcttcgccg catgtcgttt ccaagcgctc aaacggcaaa
    14281 tcggcctgga taagggcctc gatctccgcc tcgatacgcg ggaaatcctc cggcgagagc
    14341 cggtgctcca gatcgaaatc gtaatagaac ccctcttccg tcgaggggcc aatatccaac
    14401 ttcacgttgt caaacagatt gcataccgca tgcgccatca catgggccgc actatggcgc
    14461 atcacatcga tcgcttccac tccaacctcc atgccggatg cccgtaacag ccaccggaac
    14521 cagtcatatc cgaacaggat gaagtgtccc gtatccgtcc gcggttgtca agtgggcgct
    14581 tcggtgaacg tagatgaaaa acaaggttgg ttttatgaag gttcatgttt ttcacggtgc
    14641 ggaccccgag acttggaacc ccaagccaga aacccgacaa agcgtcacgg ttcggcgttc
    14701 accaaagttg aatcgctccc gggtgggaaa tcggttgact ccgtcaggac tccgcaccca
    14761 taatgagacg ttgcggtgag aattctggtc cggacagggt ggagtcggcg ggaggcagtg
    14821 aaagggaggt cggaaatgcg aggtcgtctc gggttggcgt gggtgttggc gggaagtctg
    14881 gctttggcgg agggggtggt ggagagcgcg aaaaccctgc cgttggcgca cgagacggat
    14941 gtggtgatcg tgggcggctc ttccggtgcg gtggcggccg cctgccgcgc ggctgccgaa
    15001 ggggtgcggg tttttgtggt cgcgcctcgc ccgtatctgg gcgaggatat ggcggggaag
    15061 ctgtgtctgc gcacgggggc ctcggaagat ccgcgctgcg ttttgcggcg ccggatgttc
    15121 acgacgacgc aacttccccc gtcgctgccc ttctcctacc ggagtgaccg tgccgccgcg
    15181 tcgaatcatc cggacccgca tcaccgcgtg ttgtcggacg gagtgtggca ggatgccgtc
    15241 acggagagtg cccagttcgg cggtgatgtg acgctgacgc ttgatctcgg ggcgctgacc
    15301 gaccttggtt cgttggtcgc ctgcgtgttt cggcgcgacg gggccaacgg gttccgtacc
    15361 ggcgcgctac acgtttgggg gagcacggat gcggaaacgt gggcggatct gggtaagggg
    15421 atcgatcagg aaaccgaggc cggatccgaa cgcgccagat tcagagtccc gctttccggt
    15481 cggtatcggt atgtgaaggt tctggccttg gtggcgcccg gtgtgaagcg gcagttgctt
    15541 ggcgaactgc tggtgtaccc cgttgccgat ccgtcggctg gcgcgggact gtcggaccgt
    15601 acgacgccgc ttcaggtgaa gaaagttttg gatgaggcgc tgttgcaggc gggagtcccg
    15661 tttctcaccg gcgcgctggc gaccgagacg ctgacggacg aaaccggtgc gctggcaggg
    15721 gttgtgatcg ccaaccgcag cgggcgtcag gcgatccgcg ccaaagtggt gatcgacgcg
    15781 acggaacgcg gcatccttgc ccgtgccgcg ggcggtcagg cggaaccgtt cccggccgga
    15841 cactacacgt tcacccgtcg cattgtggcg ggcgaggcac cgcgcgcgga gggggtgcgg
    15901 gtgcgggagt tgttcggggt ctacccggtg cgtgtcacgg gcatccgtcc accggagggg
    15961 catcccggca cgatcacggg acgtctgttc gaatgcgaga tcgacctgcc gatgcgggac
    16021 ggttcagcac gcgcctttgc cgaggcggag caacgcgcgc gcgatctgac ctttgttccc
    16081 acgcagttgg aatcagcgga tacgctttct ctgcttccgc ccgacttcat tcggggcaag
    16141 gccgctgtac gggcgccctg ggccggggtt gacgcgcttg atctggatgc gtttcaaccg
    16201 gctggcacgt cgtttctgtt tgttctgggg ccgatggcgg atgtcgcgcg gccaacggtc
    16261 gagcacttga tgcggcccgg taatctgatg gccttgggtg agcggatcgg cgcggcggcc
    16321 gcacggctgg cgaaacagcg cccggcaccg cagggcgtgc gtgcaggggg gagtccggat
    16381 gcgcaaagcg gcggagtcac ggtcggcgaa catacccgca atctggcggc ctatctgcgg
    16441 aacgcctccg gcacagttcg ggtcgagccg cgtgaattgc cggttctggc tgagtgcgat
    16501 gtgctggtcg cgggggcggg caccggcggg gcgcccgccg gaatcgcggc cgcgcgtcat
    16561 ggtgcgcgga ccctggtctg cgaatatatt catcagatgg gcggcgtaca gaccgacggg
    16621 cttatcggcc gctactattt cgggaaccgc gtgggcttca cgctggatat tgaccggggc
    16681 gtgaaagaga ccggggcggt cttttcccag tgcaagtccg agtggtaccg cagccagaac
    16741 cggaagggcg gggccgagat ctggtatgga acgatggtca gcggggtggt ggtggagaac
    16801 aaccggctca ccggcgtggt ggtggtcatg cccgacggca cacgcggcgt ggtgcgttgc
    16861 cgcgttgcga tcgacagcac cggtaatgcg gaactcgcgg ccatggcggg cgaaaagacc
    16921 gagttcatca ccgccgctga agtggcgttg cagggtgtgg gccagacccc gcgcaggatg
    16981 ggcgcgagtt acaccaacac cgatatcggc tttctggatg atacggacgc gtccgacctc
    17041 tgcttcttcg cgttgcgttc ccggtacagt atgcgggagg ggatctggga tcaggcgcag
    17101 atcatcaaca gccgcgaacg ccgccggatg atcggtgcct tttacatgtc gccgctggat
    17161 gtggtcaacg gacggaccta tccggatgtg gtggtccagc cctacagcaa cttcgactcg
    17221 cacggccata cgacccacga gcagtttttc attgaggatc ccggccatca cggaatgaag
    17281 gtcaacttgc cgttccggtg tctgctgccg aaaacggtgg acgggctgct ggtgaccgga
    17341 ctcgggatca gcgcacaccg cgacgccatg ccgattttac gcatgcagcc ggatgtgcag
    17401 aatcagggct atgcggcggg cacggcagcg gccatggcga tcaaggcggg tgtgccggtg
    17461 cgtcgggtgg acatccgtgc gctccagcgt catctgatcg aaatgaagat cctgccggag
    17521 acggtgctgg acatggagga ttcctttccg cttccggacg cctgcttcac cgaagcggtg
    17581 gccgcgatcc ccaatggcta tcagggactg tcggtggtgc tgacggaccc cgcgcgcagc
    17641 gtgccgctcc tcaaagcggc ctacaccgcg gcccgcgtac ccgaggcccg tttggcttac
    17701 gcgcatgtgc ttggcatgat ggggcatccc gatggtgaga cggaactgat tgcgcgggtt
    17761 cagggcatgg cctgggacaa ggggtggcat tttcgcggga tggggcagtt caaccgcagt
    17821 gtgagctggc tggacagtta cctgatcgcg ttgggacgct gccgctccgc acgcgcggtg
    17881 cccgcgattt tggacaaggc gaaggccctg accgcggccg acgccttctc gcatttccgt
    17941 gcggtggcct tggcgctgga ggcgatcggc gatccggcgg gcgccccggt tctgggacgg
    18001 ctgctcgcta cggagggcat cggtggttac gccatccgta tgggacccga actcccggtg
    18061 attcccaact acgcgaatca cgaaggcgac cgggagcgtt ccgcatgtct gcgtgaattg
    18121 gcgttggcgc gggcgctttt ccggctcggc gatttcgagg ggcgtggcgt gcgcacgttg
    18181 cgggcctatg ccgaggatcc gcgcggcgcg tatgcgacgc atgctgcgct ggtgctgggc
    18241 aggtgatgcg gagcagcggg ttacatgtta cggcttattc gcgtgcgtga acgggtaagg
    18301 ttaatctggc gtattcggtg tgatgtgata cacttttttc tttcattcgg atggatcgga
    18361 aatatccgca ggaacaggga aacgcatgaa tctgatcaga acactttttg gaaccaaaaa
    18421 cgaacgcgat ataaaacgcc tccagccgat ggtcactcgg atcaacgagt tggaaaaaga
    18481 gtatcaggca ctgtcggagc agcagcttca ggccaaaaca caggaattca aggagcgcgt
    18541 ggctgcggga gagtcgctgg atgacttgct gtgcgaggcg ttcgccgtgg tgaaaaacgc
    18601 gtgccgtcgg ctttgcggca ccacccgcga ggtgtgcggg cacatgctgg cttgggatat
    18661 ggttccgttt gatgtccagc tcatcggcgg gatcgtgctg catcagggca agatcgcgga
    18721 aatgcagacc ggtgagggca agaccttggt cgccaccctg ccgctgtacc tcaacgcgct
    18781 ctccgggaac aatgtccggc tggtgacggt caacgactat ctcgctcggc gcgacgccca
    18841 atggatgggg catgtctatg agtatctggg gttgacggtg ggatgcatcc agaaccacat
    18901 ggggccggct gagcgtcggg aacaatacgc atgcgacatc acctacggca ccaacagcga
    18961 gttcgggttc gactaccttc gggacaacgg catggcggtg gagccgtcgc aggtggtgca
    19021 gcgcggccac catttcgcga tcatcgacga ggtggacagc attctgattg acgaggcgcg
    19081 cacgccgctg atcatctccg gtccggtcgc gcatgtgtcc acggcgcagt acacggaact
    19141 caggccgcgt gtggagcggc tggtccgcga ccagatggag atctgtaacc gcctgatcag
    19201 cgaggtccgc gaggccattg aagctgcgga cagcgaaacg gcgatgttca agctgtatca
    19261 ggtgtaccac ggcatgccca agcacaagca gttcctgcat ctgttggagg accccgcgac
    19321 gcgcaagctg catgagcagg tcgagagcat gatgctctcc gaaatgcgca aagaactggc
    19381 ccgcgacctg cgcgagcaac tgcttttcac gatcgatgag cgtacgcggg aggtcgcgct
    19441 gaccgaccgg ggcagtgaga agctctcgcc gaccgatccg gaaatgttcg tcatgcccga
    19501 tctggtctcc gccatgtcgg cgttggatgg cgacaccgca ctgggcgcgg aggagcgcgc
    19561 caaacggcgg cagcagattc aatccgattt catggtgcgc agcgagcgcg tccacaatgt
    19621 ggaccaattg ctgcgggcct actgcctcta cgagcgggac gtcgattatg tggtgcagga
    19681 taaccacgtc tatattgtgg acgagttcac cggccgcgtg ctgcccggaa ggcgttggag
    19741 cgatgggctg catcaggcgg tggaggccaa agagggcgtt gaaatcgagc gcgagacgca
    19801 gaccctcgcc acgatcacca tccagaacta tttccggctg tacaaaaagt tggcgggcat
    19861 gaccggtacg gcggagaccg aggcgtcgga gttccacgac atttataagt tggatgtggt
    19921 ggtgatcccg accaaccggc cgatacggcg cgtggacggt aacgatcaga tctacaaatc
    19981 gcagcgcgaa aaatacaagg ccattctcga cgaggtggag aagcgccacc ggaacgggca
    20041 gccggtgctg ttgggcacgg tgaccgtgga cacctccgag atcctcagcc gcatgctgcg
    20101 catggccaag atcccccaca acgtgctgaa tgccaagaat cacgcgcgcg aggccgagat
    20161 cgtgtcgctg gccggacagc ccggcgcggt caccatcgcg accaacatgg cgggccgcgg
    20221 tacggacatc aagctgggtg aaggcgtggt gtgggtgccg gagacgacga tccggtcgca
    20281 gatcacgctg gaaaaccgtt atgacaacgg gaacaagacc ttgcgcaagc tgctggagga
    20341 gaagccgtgc ggactgcatg tgatcggatc cgaacgtcac gagtcgcgcc gtatcgaccg
    20401 gcagttgcgt ggccggtgtg cgcgtcaggg tgacccgggc tcctcccagt tttacatttc
    20461 gctggaggac aacttgatgc gcctgttcgg gtccgaccgc atttcgggta tcatgacccg
    20521 cctcggcatg caggagggcg aggcgctgga gcacaaatgg cttaaccgtt ccgtggaaac
    20581 cgcgcagcgc cgcgtggagc agcagaactt cgccatccgc aagcgcacgc tcgaatacga
    20641 tgatgtgatg aacaagcagc gttcggtcgt gtacgacctg cgcggggacg tgctgaccgc
    20701 tgaaacggcg cacaaccata ttttggatgt gttcaacgac ctgatcctga cgcagagcga
    20761 gaggtacctt tcctcggcca aagacgcgat gccgcaggaa ctggtgaggt gggtgacgga
    20821 caccttcccg gtcgcggtgc gggaggaaga gatcgcggcg ttccggggcg agcccgaaaa
    20881 agccggagaa ttgctgtaca cgcgggtcgc ggaggcgtat gaactgaaat gttccatgga
    20941 ggatcccgct gtgctgccga tcatggaacg ctcggttttt ctgagttgta tcgaccagca
    21001 gtggcagagt tacctgcgcg ccatggacga gttgcggcat ggcgtcaacc tgcgcgcgta
    21061 cggtcagcgc gatccgctgg tggagtataa gcgcgaagcg ttcacgatgt ttgaggaact
    21121 gatgaccaac atcaaaaccg atgtggccac ctcggtgttc cgcgcgacga cgatgcagaa
    21181 tttccagcgc atgatggcgg gtccgggcgg ccgggtccag atggtgcaca acgatatcaa
    21241 catactggcg ggcggcgaga ccccgcagcc tgcggccgga caggcaggaa ggggtctgac
    21301 ggcggcggat gtgccgcagg ccgctgtggc cggattcgac gcgatcctgg acgccatgga
    21361 tcgcacgggg cagagtgcgc cgcatgcgac gacgggtccc ctgatcgcga agccctcggt
    21421 ggggcgtaac gatccctgtc cgtgcggcag tggcaaaaaa tataagaaat gctgcggcta
    21481 ggggttgtaa tctgactgtt gcgcagtcag gttacaggtt agcccgccgc gcgccggagc
    21541 cgcgagaaaa ctctgggata catttttatg acagg
//
LOCUS       DAOSLE010000029        21458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2706_length_21458_cov_7.649629, whole genome shotgun sequence.
ACCESSION   DAOSLE010000029 DAOSLE010000000
VERSION     DAOSLE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21458
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2706_length_21458_cov_7.649629"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1179)
                     /locus_tag="PK576_03575"
     CDS             complement(154..1179)
                     /locus_tag="PK576_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60701.1"
                     /translation="MFRLEDGAWMQASASTVVGNDLATNNIAYIGTNAVFDALGAVTI
                     GNSASTAFNQMIVDAGTFYVTNNPSARNIAVGNRGFGNRLEVVNGATVYMTNVNFYVG
                     SNSDSNVLVIADGAKWREEEEGLAKRNWGIHGIGSYNGIIVDNATLELPQNILRIGNN
                     ANSSNNWVRIVNNSIVPIYRTIMGEQGTDNTLEVANSLFTMWGSLQVGHTTATSYRQR
                     LWVHGTNTYITVNEGLDIGPDCELKFTIEGEGFLSNTVMYVNSLWPTETVTADHPYTV
                     RIEAAHGAKPGVYTLMQSKSANNLHWGAEDLFKLEYDPKYIQIVQRDSSKLVVKVKHH
                     GMTVLLR"
     gene            complement(1309..2217)
                     /locus_tag="PK576_03580"
     CDS             complement(1309..2217)
                     /locus_tag="PK576_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60702.1"
                     /translation="MRNRINMFFLFLVGFTFAIPTMATDMTWSNTAGDYDLGNAQNWG
                     GTLPEGSNHTATISTKVTDPAWLKLSAVSTLQPYAITVKNDIDLDLGTGSVFTALYRV
                     YFNTDSALTLRSGTFGVDWESASGERFFMGDNGNGGGSITVDGPDAIITSSQKNTLQI
                     GTNRKDYRLNILNGATLQANITLGNTLLQASNNVVWIEGPGSKHLVPSDSTVAPIAIG
                     STAPHCSYILTNQASLVVERDAGIFVGSFYSAVPGGLAAMHVLDHSTVDTLGSLYIGN
                     YSGTNSLYVSGASTFNCAEFRLGENP"
     gene            complement(2481..3014)
                     /locus_tag="PK576_03585"
     CDS             complement(2481..3014)
                     /locus_tag="PK576_03585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE60703.1"
                     /translation="MVYAFPRFFGCPKSPAADSSRWVLRIADRSLIKTGTGTLTLTGA
                     NTYTGGTVVNSGSLVTTNLSTLGDSPSVTLALGTTLEVSAGGETFNGDLTVNVSSNSA
                     SLLAVNGSLTLGPDSSLTVAPGSQFNRRKTYTVVTCAALNGTFGSVNGLPKAWFARYV
                     SNRVELFYAEGTVIRLY"
     gene            complement(3048..3296)
                     /gene="acpP"
                     /locus_tag="PK576_03590"
     CDS             complement(3048..3296)
                     /gene="acpP"
                     /locus_tag="PK576_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018291358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOE60704.1"
                     /translation="MASKLEERVKEIIVEQLGVNAEQVTPEASFIDDLGADSLDTVEL
                     IMAFEEEFGAEIPDEDAEKLTSVGKVFEYLKEKGFGQD"
     gene            complement(3360..4097)
                     /gene="fabG"
                     /locus_tag="PK576_03595"
     CDS             complement(3360..4097)
                     /gene="fabG"
                     /locus_tag="PK576_03595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOE60705.1"
                     /translation="MLDGKVAVVTGAARGIGQAIARRLAQEGADIVLCDLQAEWLAES
                     AGMVQDCGRKALPLAVDVGDSEAVNACVQEAVKEFGKVDILVNNAGITKDTLLVRMSD
                     ADWDDVIRVNLKGTFLFTRAVAKNMMRQRSGAIINIASISGLIGTAGQANYAASKAGV
                     IALTKSTANELAGRGVRANAIAPGFISSKMTDALSEDVRKQYLARIPLGRFGTVEDIA
                     HAVVFLASEQSSYLTGQTLNVNGGMVM"
     gene            complement(4132..5058)
                     /gene="fabD"
                     /locus_tag="PK576_03600"
     CDS             complement(4132..5058)
                     /gene="fabD"
                     /locus_tag="PK576_03600"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOE60706.1"
                     /translation="MSDRVCVFAGQGAQVPGMGKDFASDPEGMALFECANRVLGFDLA
                     TLCFEGPAEALTKSNVCQPAIFTVSVAAFRAFQKRCPRVSFAMAGGLSLGEWTALHVA
                     GVLEFEAALTILEARGRFMQQACEEQASGMISIMGATAEQLQTLCDRTGLTVANINSD
                     AQVVLSGLKAGVESAVAVAAELGVKAIPLNVAGAFHSPLMASARSKLAEVLEGVTFRA
                     PSMPVLANVTGAPHSTDPSAIRDAMLRQITDSVRWLDCVRHAVAAGAGGFVEFGPGKV
                     LSGLIRRIDKGVDTLNVADVTSLDAAVAALGA"
     gene            complement(5225..6256)
                     /gene="plsX"
                     /locus_tag="PK576_03605"
     CDS             complement(5225..6256)
                     /gene="plsX"
                     /locus_tag="PK576_03605"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOE60707.1"
                     /translation="MRIALDVMGGDRAPEEIVHGAVEASLKFKDVSKILMVGDEAAIR
                     AELAKYPKADLTRLDIVHAPDSISMGESPVVAVRKKKRCSINLCMDLIKNREADAMVS
                     AGNSGAVAAAAMFCLGRMPGVLRPAIATVIPTRQPKRPLLLLDAGANTDCHEAWLAQF
                     AVMGNAYSQAVLKRTEPVVGLLSIGTEDCKGNDMTKKAFHLIQRSKVKFRGNVEGHDL
                     FLGKIDVVVCDGFVGNVVLKTTESVAHAVGYWMKREFFRHPVRLLGALLLKGALETLK
                     RRMDPEIYGGAPLLGVPGTVIITHGSSSYRAIFHAIIAGAAAAANNLSDTIARRIAEM
                     EMPDEGAGV"
     gene            complement(6357..6536)
                     /gene="rpmF"
                     /locus_tag="PK576_03610"
     CDS             complement(6357..6536)
                     /gene="rpmF"
                     /locus_tag="PK576_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOE60708.1"
                     /translation="MAVPKRKKSKMRKNQRKGHIKAKVAQVQACPSCGASQQTHRVCA
                     SCGMYKGRKVLTVAE"
     gene            complement(6585..7043)
                     /locus_tag="PK576_03615"
     CDS             complement(6585..7043)
                     /locus_tag="PK576_03615"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOE60709.1"
                     /translation="MIVDVARLEAGGEAYCGETGTGLLELGESEWVTPVGGMRYALRV
                     EMAGDELLVRGEVRQSLRCVCSRCAVEFDTEVAVPDFLCSLERDTLDEFVDLTSELRE
                     ATILALPGYPVCRETCQGLCMTCGVNLNTASCSCRKQDRDRRWSVLDALS"
     gene            complement(7033..7584)
                     /gene="coaD"
                     /locus_tag="PK576_03620"
     CDS             complement(7033..7584)
                     /gene="coaD"
                     /locus_tag="PK576_03620"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014236132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE60710.1"
                     /translation="MEKIVVYPGTFDPLTLGHFDLLRRSASLFDQVIVVVADVSVKAG
                     GMFNLQERMAMIRESVDEVGMTNIEIDRLDCLLVDYCRRRKVRVVVRGVRVYSDFEYE
                     FQMALTNRKLAPEIETLFMMPNENYSYVTASTIREIARYGGDTSAFVPAPVQKYIRAH
                     MSRHPDPHIRAGGGIGNTGAFDR"
     gene            complement(7627..8202)
                     /gene="rsmD"
                     /locus_tag="PK576_03625"
     CDS             complement(7627..8202)
                     /gene="rsmD"
                     /locus_tag="PK576_03625"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOE60711.1"
                     /translation="MRITGGEWGGRKLKAPAGDRVRPTQDRVREALFSMLMGAVPGAL
                     FIDLFAGTGAVGLEALSRGASRVVWVEQDPRYAAVLQENVVAVTGKPGEVCRCGVLRW
                     LKGAGCGMRADVVFADPPYAADGDSGFAQIMGLLETNRVVRPGGIFVAEMPEACAAEE
                     TAGWRLVRDRTYGHTRLAVYRMGTEGTETTD"
     gene            complement(8377..9399)
                     /locus_tag="PK576_03630"
     CDS             complement(8377..9399)
                     /locus_tag="PK576_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60712.1"
                     /translation="MIALAAGYQHFEDGAPFAEIALRHPAVKEVYFPWVDEPSGRPKL
                     GYEEEDDPAELEAALQRDLGRLREAGMKLDLLLNANCYGEKAMSHEFEERIITILEQL
                     ISWRCKPEIVTTASPFVARTLKKVFPEIEMRASVNMRLTTLQAFKYLAPLFDSYYLGR
                     DVQRNLDTVSRFSDWCHVNGKKLCILANSGCLRNCPWQTFHDNLIAHSDAAMNVENVK
                     GWSPHLCWTLYKDPKNFPHILKATWVRPEDLYRFEGLVDVVKLATRQHANPDMVIGAY
                     ERGGFEGNLLDLFEPGFSPAFFPTALDNAAFPPDWFDRTSSCSRECAECGYCEQVLEK
                     VKVNIG"
     gene            complement(9772..11229)
                     /locus_tag="PK576_03635"
     CDS             complement(9772..11229)
                     /locus_tag="PK576_03635"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60713.1"
                     /translation="MSITRRGFIGGVSASIFIGGCTTVKGPRKIAANAKVNVAIIGCG
                     LIAKTTNAPGFLQDPRCRVTSVCDVVEIAPDYFYGSRNTNLGVEDFVRADGRYRRDVC
                     GWRVLRDVVNAHYGDKACKVVFDWRTIINDPAIDAVCICTPDHWHAIMSIAAMKAGKH
                     VFCQKPMTLSLAEGQTMVRVAKETGVTFQVGNQGSTNPAYRLTQEIVLNGYAGKVKSA
                     TICIPACDHWAGHGRSAARLPRPAYLSEAGWNMWQGPARHWEDNAYIPSIHDPTCWRF
                     NSRYGGGMIPDFGAHEFDQLNRGLGTQFTGPVAVENVETDYATGTDRDIFSWPSEFKF
                     DFVFANGVRCHVRKIDKEAGWPRQTIFHCEKGDVGSYAGKGKVPECLKNFRESDLTDK
                     DIKLHRPPAERDGSKFHESDFLDGVLTGSQDVASTCEMGHRTISMAHIANICARMQLS
                     SLKWDPKSERFVGAYADEANRFLSAEYHNGWELGA"
     gene            complement(11344..13326)
                     /locus_tag="PK576_03640"
     CDS             complement(11344..13326)
                     /locus_tag="PK576_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60714.1"
                     /translation="MDRMPTRKITSFALFTLLATGCAVAGTPVVIDVPAGTKLTVIRD
                     QIRALSVAQRVQGVEVRLARGVFEMEEGLALEPQDSGTPDAPIIWRGAACGGTRLIGG
                     RAIPADVFKPDPERPGVLVADVSDWQANWPAKPTETVHPYPVPELYVDGKRMMPAGWP
                     DPVSGELDDWEDGWATFDKVVNPGTNGVDCGTFVYRGLRPDRWVNEKYAYVFGFWKTE
                     WRADEMPIASIDDKEKTITLGGFHFYGLGGINSWSNRPRRWRVLNVLCELDTPGECYV
                     DVEANKVYLIPRHPLDAQSQVRLCWQDEDIVSLRGVANVTICDLTIGPAWKNAVWGSA
                     VTNVVIRNLTISGTRLKGIEFSEADHCRIADCTMYDLGAGGVKVSGGDRRNLIRGENV
                     VENNLIHDFSKIRMTYAWAIQTWGVGDVVRHNEMYNTIHEALNVDGNDCLIEYNVISN
                     AVTGTDDSGAFHCGRNISCRGNVFRHNLFANIGNPEFRAQAALYLDDGHSGWTIDGCI
                     FRDVFRNEDKRGFGAIHCNGGYSNVVRNCLFINCGRALGSRWVTDEIWNNWLKEPGWI
                     RRLKREVDIEGSVYQSRYPELKGVMQGFPRRHRMQRCEDNVVVHCREFKKGDNWVTNQ
                     WNAVVNELPPGDVNAACRQLIPHFQPIPFEKIGRYR"
     gene            complement(13340..15118)
                     /locus_tag="PK576_03645"
     CDS             complement(13340..15118)
                     /locus_tag="PK576_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60715.1"
                     /translation="MKKGTLALLGALTLGLNVRADTSPDPTGGYDISVDYSLVAGLVE
                     YTNIYNQTAWTGMDPGYLTSPSQGVTSLWIRVSTDSNANLYYEDGDSYMKMLSYCGYI
                     WNNSPTNETWTFICCMDANSKLFIDGELKIDQNTRGYCKTGNVVMTPGAHAFEFRYRR
                     DGGNSGAIAANIMQSIDDEVFTGFAGNDGGEWKSKFGFAIDRQGRRSHNYRDYVSPVD
                     PGDGSLFTYTTNLNGIASLYVQQGAFRYPDPDVDYSRVAGLGEYTNLYNGVAWDATEP
                     CYLTSPSQGVTSLWVRVSTDANAHLYYQDGDAENKMLSYCGYVWNDSPTNETWTFACC
                     MDAHSKLFIDGELKVNQNVRKTCRTGNVVMTPGAHAFEFRYRKLGGYSGAIAASIMQY
                     LDDEPFTSYVGNNGGEWKSKFGFAIDRQGRGSHNYRDYVSPVDPGDGSLFVVTTNRLE
                     VSSLENRIDFLAMKFAPNTVLDLNAEGLTTAVSSIEGLPTVTNGNLTVTGTWTIPAVD
                     IAAGTVLNASGSLTLSAGAMPIVSNVAALPRNTVIIARAAGGLFLPEGIDGLALDPKG
                     KFVLCQTSPTELAIKYSVQGLLIMYK"
     gene            complement(15141..17150)
                     /locus_tag="PK576_03650"
     CDS             complement(15141..17150)
                     /locus_tag="PK576_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60716.1"
                     /translation="MNRCVIPFVCGILFALSMRLEASTITRDVAVDPITTRWTFGAHE
                     PFTMYRRIGRRSTGGIYGGSKWVKDWLDWWDEHAPARMEEIGLNALHSRFYKGMGWEV
                     EKKDFPNVKKFVDNCHKHGVTVLAYVQFATLYHEVMQKEIPDIESWAAIDERGMKMIY
                     SRQAARWLPCIVNEKWVEYTEKILTIAMTEGGFDGVMFDNAFFFGCHCPRCQEAFRRH
                     IHSLPDLEERFGFAEMPGLRIPQAFRPENGDEYDPVAQEYYRWRVQCVNAVMKRFYRH
                     IKSLKPDAIVSANSGSFRSTDNYHARSVDVIDMADGGLDLLMMQTLNFPAYLDDPKFP
                     GQHYIVNRQRDLRMARDLGKQIVALSDGAAGSDALDESMYLRPLVEDLVWGGIPTDRT
                     VMSPVRREGFIDEERFAMRKKVLAEFDAFVKANRAAFTGKPYLPVRVFYPSAACGLAV
                     SMNLAVSTVDEILLRHHMPYGYVIVKGAEAPSIPADCEVLVLPNSKWMSDAQVSAVVD
                     WAKKGGRLVVTGRDTGLRDENGAERFTNPLDCVRKLPNVDWRNDIDVAQKNALGWITR
                     VLPPKDGGRAFMAGLGRVGYRNRFVFENLPEEVFVDMREVSDGVVIHLVNYHPEVQIK
                     GARLVVPSGAKATAEEPFGTRRQAVPLGKDGMLPAFGQYLMVRIY"
     gene            complement(17170..18978)
                     /locus_tag="PK576_03655"
     CDS             complement(17170..18978)
                     /locus_tag="PK576_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE60717.1"
                     /translation="MILLACGILFASSMRLEAARPAFYLPGRLLAAPGPVCNTYHAAS
                     PTVDALEASLAARDAAVDAEWLAVPDKTAFVAKRTEFKTDFRRVIGYDEIQRTPLQAK
                     TTGVRVFDAFRIEKVLMESAPGEFIATLVFLPDAKKFAPPYAGFIFIPGHNDRGKGGK
                     DYLHTCELGARNGLAVITYDPLGQGERSQGAGLRSADEHVRIGAYAVLLGETTATYML
                     RDAVRVFDYFESRPDIDRNKLGVCGNSGGGTMAAFFMAVDDRVKAATPSCYLSSARKH
                     LLTCGPQDAEQNFFDAFSWGFNHAALMFAAECPVLINAAVEDFFQIEGSRSTHRLVKE
                     VAAKVGLPADWYELSEAPGPHKMSKTHRERSVSFLLKHLTGESRQVAETETTEFTADD
                     CTVTPDEEVSHLVGFKSIYDTIAEKFVARGVSVDNAAQNAKPLVAKELIGKDCSGVLA
                     TLKGELAKDRRATLRLGGLAGAGEVTATLFADAGRYVIRSERKGKRSYYERRGDDEVV
                     AVDLYIAGRSLVALRAAEILTLAAELEKRTGVKPMLVAEGRYVTIAKFALAADPTAFV
                     GVEYRNEPKSFLDSLKAREYLSFADSGALYSGKAGR"
     gene            complement(19471..21417)
                     /locus_tag="PK576_03660"
     CDS             complement(19471..21417)
                     /locus_tag="PK576_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60718.1"
                     /translation="MRLEASPIARDAAIDPITTRWTFGAHEPYAMYRRVGRICTGAID
                     GNALWVQDWLDWFDLHGPEKMEEMGFNFLHSRFYKGMGWEVEKKDFPNVKKFVANCHA
                     HGVRALAYVQFGTLYPEAMRRENPQIDSWAGVSETGGHNLYGGMYFRWMPCLNCREWE
                     DYLKRMCSIALVEGGFDGIMFDNVYDVPCYCQRCEKAFREHLKTVPDREARFGFDDLD
                     YMMLPKIRISVETTLEVKDPVAQEWFRWRADVMTGVMTRLRDHIKHVKPDAIVAGNPS
                     PYRNRAGALSKAQNMAELCRAFDCIIMQNANFPEVREGGVIRNRVRDLKFAQDMGQRI
                     VALCDNVEHLHADRESKFLLPMVEDLVFGGIPTDRTSIFPSPEPGFINRKVFEARKPI
                     HTRFNAFATAHAAELTAPAIHSVRIFYPEREIYLSERNHQGVVAAEEILLRNRVPYGY
                     LVSSPDNVMTVPPETEVVIVPGLVALSDAQVEALVAYAKSGGKLVVTGDAGRYDDWIA
                     QRRANPLLPQLQGFANVVCRVEADLIRGAVMGWGYTVPPPVDGGKKLMEDLRKVGWRP
                     PVSFDNLPPYVFAEYRRLVSGGLVVHLVNYKPEERIVGARIALPKGVSAVLEEPFGNE
                     PRKVTLEGDGVLPTLGMYVMIVVK"
BASE COUNT         4536 a         6872 c         5710 g         4340 t
ORIGIN      
        1 aggcggtggt gttccgtggt gaaacagtcg atcccgatcc cgatagcgac cccgaccccg
       61 atttggatgg aaccgataaa accagaaacc caaaatcttg agcgcgcttg acggttccaa
      121 acacccgaaa tcaaaaggag acttctcgcc agatcagcgt aaaaggaccg tcatgccatg
      181 gtgcttaact ttgacgacga gtttggaact gtcccgctgg acaatctgga tatacttcgg
      241 atcgtactca agcttgaaaa ggtcttcggc tccccagtgg agattgttgg cgctcttcga
      301 ctgcatgagc gtgtagacgc cgggtttggc gccatgagcc gcctcgatcc gaaccgtgta
      361 cggatgatca gcggtgaccg tttccgttgg ccagagggaa ttcacataca tcacggtgtt
      421 ggagaggaag ccctcgcctt cgatggtgaa cttgagttca caatccggac cgatatccag
      481 gccttcgttt acagtaatgt aggtgttcgt gccgtgcacc cagagacgct gacggtaaga
      541 ggtcgcggtc gtgtgcccga cctgcaatga cccccacatg gtaaagagcg aattcgcgac
      601 ttccagtgtg ttgtccgtac cttgctcgcc catgatggtg cgatagatcg gcacgatcga
      661 gttgttgacg atccttaccc agttgttgga cgaatttgcg ttgttcccga tacggagaat
      721 gttctggggg agttccagcg tcgcattgtc gacgatgatt ccgttatacg atccgatgcc
      781 gtggatgccc cagttgcgct tcgccagccc ttcctcctct tcacgccact tggcgccgtc
      841 cgcaatgacg aggacattgg aatcggaatt cgatccgacg taaaagttga cgttcgtcat
      901 gtacaccgtc gcgccattca cgacttccag acggttgccg aagccgcggt tcccgacggc
      961 gatattgcgg gcagacgggt tgttggtgac ataaaacgta cccgcgtcca cgatcatctg
     1021 gttgaacgcc gtcgaggcgc tgttgccgat cgtgaccgca ccgagcgcgt cgaacaccgc
     1081 gttcgtgccg atataggcga tgttgttggt cgccaggtcg ttgccgacca ccgtgctggc
     1141 ggaggcttgc atccacgcgc catcttcgag gcggaacacg ttcgactgcg tgtacgtgcc
     1201 gccgatatag acattaccgg aggctgcaag gcgcgttccg tcgccgcgta tcgttgcggt
     1261 attagcaaag gaggggccaa cgccggcatt tttaagagcg gtcttgctct agggattttc
     1321 accgagacgg aattcggcgc agttgaaggt cgaggccccg gagacgtaaa gcgagttggt
     1381 gccactgtaa ttgccgatgt agaggctgcc gagggtatcg accgtcgaat gatcaagaac
     1441 gtgcattgcc gcaagtccgc cgggcacagc cgaatagaaa ctgccgacaa agataccggc
     1501 gtccctctcg acgacgagcg aggcttggtt tgtgagaatg tagctgcagt gcggggcggt
     1561 cgatccgatg gcgattggag cgaccgtcga atcggatggg acgaggtgtt tcgaaccagg
     1621 gccttcgatc cagacgacgt tgttggatgc ctgaagcaac gtgttgccca aagtgatgtt
     1681 cgcctgcaat gtggcgccgt tcaaaatgtt gagccggtag tccttacggt tggtgccgat
     1741 ctggagggtg ttcttttggg agcttgtgat gatcgcatcc ggcccgtcca cggtgatgga
     1801 gccgccgccg ttgccattgt cacccataaa gaaacgttcg ccactcgcgg actcccaatc
     1861 gacgccgaac gtgccgctcc taagcgtgag tgcggagtcc gtgttgaaat atacgcggta
     1921 aagcgcggtg aacacactcc ccgtgccgag atcgaggtcg atgtcgttct tcaccgtgat
     1981 cgcatacggc tggagcgtcg agactgccga cagtttcagc caagccgggt cagtcacttt
     2041 cgtgctgatt gtcgccgtgt ggttggaacc ttccggcagc gttccacccc agttttgggc
     2101 attacccagg tcgtagtcac cagcggtatt ggaccaggtc atgtccgttg ccatcgtcgg
     2161 tatcgcaaaa gtgaacccaa cgagaaacaa aaaaaacatg ttgatacggt tacgcatgat
     2221 cacctctttt gatttcatcg cttcggacag accggaaccg caacggttcc ctcaaacgcc
     2281 cgaacgccag ctattatgcg gaggaaagcc gaaacgtgtc aacctctgaa agtggcgaag
     2341 ttggggtgac gatcttcggc acgatgatcc tagccgtttt accatacctt cgggcgtccg
     2401 aaccgcaggc ggtccatgca gtacggcggg acgtccacag ctccgcgcca gcggcgcgga
     2461 gccggagcgc cgaaaccgtt ctaatacagc cggatcaccg tgccctccgc gtagaacagt
     2521 tcaacacgat tggacacata acgcgcgaac catgctttcg gcagaccgtt aaccgaaccg
     2581 aaggtgccgt tcagcgcggc gcacgtcacg accgtgtacg tcttcctgcg gttgaactgc
     2641 gaacccggtg ccaccgtcaa agaactgtcc ggccccagcg tcaggctgcc gttcacggcc
     2701 agcaggctcg ccgagttgga ggagacattc accgtcagat ccccgttgaa cgtttcgccg
     2761 cctgccgaga cctccagcgt tgtccccaac gccaaggtga ccgagggaga gtcgccgagt
     2821 gtggagaggt tcgtcgtcac cagggaaccg ctgttcacaa ccgtgccgcc cgtataggtg
     2881 ttggcaccgg tcaacgtcag ggtgcccgtc ccggttttga tcagagagcg atcggcgatc
     2941 cgcagaaccc aacgggatga gtccgcggca ggagacttgg gacaaccaaa aaaccggggg
     3001 aatgcgtaaa ccatccgccc cggttttcaa tacgttcggg aagacactca gtcctgtccg
     3061 aaaccctttt ccttcagata ctcgaacacc ttgccgaccg acgtcagctt ttccgcgtcc
     3121 tcgtccggaa tttccgcgcc gaactcctct tcgaacgcca taatcagttc cacggtgtcc
     3181 agcgagtccg cacccagatc atcgatgaac gatgcttccg gtgtgacctg ctcagcgttg
     3241 acgcccaatt gctcaacgat gatctctttg acccgttctt ccagtttgct tgccatgtac
     3301 tctctccttg atctggtttc ccttgttata cagttgcggc acatgccgcg ccaatccgat
     3361 tacatcacca tgccgccgtt gacgttcagc gtctgcccgg tgagatagct cgactgctcg
     3421 gaagcgagga acaccaccgc atgcgcgatg tcctccacgg ttccgaaacg gcccagcgga
     3481 atccgggcca agtactgttt gcgcacgtct tccgacagcg cgtccgtcat tttcgacgag
     3541 atgaaccccg gcgcgatcgc gttcgccctg accccgcgtc cggccaactc attggccgtg
     3601 gacttggtca atgcgatcac accggccttg gaagccgcat agttggcctg cccggcggta
     3661 ccgatgagcc cgctgatcga ggcgatgttg atgatcgccc cgctccgctg ccgcatcatg
     3721 ttcttggcca cggcgcgtgt gaacaggaag gtgcccttca ggttcacgcg gatcacatca
     3781 tcccaatccg cgtccgacat gcgcaccaga agcgtgtctt tggtgatgcc agcgttgttg
     3841 acaaggatat ccaccttgcc gaactccttg accgcctcct gcacgcaggc gtttacagcc
     3901 tcactgtcac ccacgtccac tgccagcggc agcgccttac ggccgcagtc ctgaaccatc
     3961 cccgcgctct cggcgagcca ctccgcctgc aaatcgcaaa ggacgatatc ggcgccctcc
     4021 tgcgccagcc tccgggcgat ggcctgaccg attccgcgcg ccgcgccggt caccaccgcc
     4081 actttcccgt caagcattcc catgatgtct ccttttccga aaaatcgaac ctcacgcccc
     4141 gagcgcggca accgcggcat caagcgacgt cacatccgcc acattgagcg tgtccacacc
     4201 cttgtcgatg cgccggatca ggccggacag caccttgcca ggcccgaact ccacaaaacc
     4261 accggctccc gcagcaacgg catgccggac gcagtccaac caacgcaccg aatccgtgat
     4321 ctgccggagc atggcgtcac ggatcgccga cggatccgtg ctgtgcggcg ctccggtcac
     4381 attggcgagc accggcatcg agggggcgcg gaaggtcacg ccctccaaca cctccgccag
     4441 cttggaccgg gccgaggcca tcagcggcga atggaacgcg cccgccacat tgaggggaat
     4501 cgccttcacg cccaattcgg ccgccaccgc caccgcagat tccacgccgg ccttgagacc
     4561 ggacagcacg acctgcgcat cggagttgat gttggcaacc gtcagtcccg tgcggtcgca
     4621 cagcgtttga agttgttcgg cggtcgcgcc catgatgctg atcatgccgc tcgcctgctc
     4681 ctcgcaagcc tgctgcatga aacgcccgcg cgcctccaga atggtcagcg ccgcctcgaa
     4741 ctccagcacc cccgccacat gcagcgcggt ccactcgccc aaactcagac cgcccgccat
     4801 tgcgaacgaa acgcggggac agcgtttctg gaatgcccgg aacgccgcca cactgaccgt
     4861 gaagatcgct ggctggcaga cattggattt cgtcagcgcc tccgccgggc cttcgaaaca
     4921 gagcgtcgcc aaatcaaatc ccaacacccg gtttgcgcac tcgaaaaggg ccatcccctc
     4981 cggatcgctg gcaaaatcct ttcccatacc cggcacttgc gcgccttgtc ctgcaaacac
     5041 gcagacccga tcactcattc gcgtctcctc agttgcttgt tctgtgccac acctgtccca
     5101 tgtacagacg tatgaggcgg cgcattttga cattttttcg caaaatgtca atttcaagcc
     5161 gtttttgaac tgatttcgga agcatagcac atccagacgc ggcggcgcga tagaaaaata
     5221 ggggctacac tcccgcgccc tcgtccggca tctccatctc ggcgatccgg cgggcgatcg
     5281 tatccgaaag gttgttggcg gctgcggcgg cacccgcgat gatcgcgtgg aaaatcgcgc
     5341 ggtaggaaga ggacccgtgc gtgatgatca cggtgcccgg aacccccagc aacggcgcgc
     5401 ccccgtaaat ctccgggtcc atgcggcgct tcagcgtttc caaggcgccc ttcagcagca
     5461 gcgcgcccag caaacgcacc ggatggcgga aaaactccct cttcatccag tatccgaccg
     5521 catgcgccac gctctccgtg gttttgagca ccacattccc cacgaaaccg tcgcacacca
     5581 ccacatcgat tttgccgagg aagagatcat gcccctcgac attgccgcgg aacttcacct
     5641 tcgagcgttg gatcaggtgg aacgctttct tcgtcatgtc gttccccttg caatcctccg
     5701 tgccgatgct gagcaacccc accaccggct cggtacgctt cagaaccgct tgcgaataag
     5761 cgttacccat cacggcgaac tgcgcgagcc aagcttcgtg gcaatccgtg ttggcccccg
     5821 cgtccagcaa caacagcggc cgcttgggct ggcgcgtcgg gatcaccgtg gcgatcgccg
     5881 ggcggagcac gccgggcatc cgccccaggc agaacatcgc ggccgcggcc accgcgccgg
     5941 agttgccggc cgagaccatg gcgtcggcct cgcggttttt gatcaaatcc atgcagaggt
     6001 tgatggaaca ccgctttttt ttgcgcaccg ccaccacagg cgactccccc atgctgatcg
     6061 aatccggagc atggacgatg tccaaccggg ttaggtccgc cttggggtac ttcgcgagtt
     6121 cggcccggat ggcggcctcg tcaccgacca tcaggatctt cgaaacatct ttgaacttga
     6181 gggacgcctc gaccgcacca tgcacgatct cctccggcgc acggtccccc cccatcacat
     6241 ccaacgcaat ccgcattctc acaccccgtt tgcgttcgca tcatgcaaag gggttccgcc
     6301 ccaggcgaaa accccttcga tgcctctccg ggcacatacc gctccttccg gccgccctac
     6361 tccgcgaccg tcaacacttt gcggcccttg tacatcccgc acgaagcgca aacccggtgg
     6421 gtctgctgcg acgcgccgca actcgggcac gcctgaacct gcgcgacctt ggctttgata
     6481 tgccccttgc gctggttctt gcgcattttc gactttttcc tttttggtac agccatgttt
     6541 ccctctctcg ttcaaaaaaa ccatcccgcg ttccgccgcc atcctcagga cagcgcatcc
     6601 agcaccgacc agcgtcggtc ccggtcttgt ttccggcatg aacacgacgc ggtgttcagg
     6661 ttcacgccgc acgtcatgca caggccctgg cacgtctcgc gacaaaccgg gtaaccgggc
     6721 agggcaagta tggtagcttc ccgaagctca ctggtcaagt ccacgaactc atccaacgta
     6781 tcccgttcca gcgagcacaa aaaatcgggc accgccacct cggtgtcgaa ctcgaccgcg
     6841 caccgcgagc agacgcagcg caaggactgc cgcacctcgc cgcgcaccag cagctcgtct
     6901 cccgccattt cgacgcgcag cgcgtagcgc atgccgccca ccggcgtcac ccactcgctc
     6961 tcgccgagct ccagcagtcc cgtgccggtc tcgccgcaat acgcttcgcc gcccgcctcg
     7021 agccgcgcga catcaacgat caaacgcccc tgtgtttcca atcccgcctc ccgcccggat
     7081 gtgcggatcc ggatgtctgc tcatgtgtgc gcgaatgtat ttctgcaccg gggcgggcac
     7141 gaacgcgctc gtgtccccgc cataccgggc gatctcgcga atcgtgctcg ccgtcacgta
     7201 actgtagttc tcgttgggca tcatgaagag cgtttcgatc tcgggcgcga gtttacggtt
     7261 ggtcagcgcc atctggaact cgtactcgaa atccgaatac acgcgcaccc cccgcaccac
     7321 caccctgacc ttgcggcggc ggcaatagtc aaccaacaga cagtccaacc ggtcgatctc
     7381 gatgttggtc atgccgacct catcgacgct ctcgcggatc atggccatgc gttcttgcag
     7441 gttgaacatg cccccggcct tgaccgatac gtccgcgacc acaacgatca cctgatcgaa
     7501 cagcgaggcg ctacgccgca agagatcgaa gtggcccagc gtcagcgggt caaacgtgcc
     7561 aggatagact acaatttttt ccataaggtt ctctctcata cggtccggcc cttacgccgg
     7621 aaccgctcaa tccgttgtct cggttccctc tgttcccatc cggtacaccg ccagccgcgt
     7681 gtgcccatat gtccggtcgc gaacgaggcg ccaccccgcg gtctcctccg ccgcgcaggc
     7741 ctccggcatc tccgccacga atatcccgcc cggccgcacc acccggttcg tctccagcag
     7801 ccccatgatc tgcgcgaagc ccgaatcacc gtccgctgcg tacggcggat cagcgaacac
     7861 cacatccgcc cgcatcccgc accccgcgcc cttcagccag cggaggaccc cgcaacggca
     7921 gacctcgccg ggcttgccgg tcacggccac cacattctcc tgaagcacgg ccgcataccg
     7981 cggatcctgc tccacccaaa ccacacggct ggcaccgcgg ctaagggcct ccagccccac
     8041 cgcgccggtt cccgcaaaga ggtcgatgaa gagcgcgccg gggaccgccc ccatcagcat
     8101 ggagaacaac gcctcgcgca cccggtcctg agtcggacgc acacggtcac ccgccggtgc
     8161 cttgagtttc cgtccgcccc attctccgcc cgtgattcgc atacacccgt actatacccg
     8221 aaataccgcc atcggcgcaa tgtcggcctg caagggttat tcatgacacc gaaaaatatg
     8281 gaagtccaag tgaccttgca gagcggcaca tccagcgccg agaattcgct cccgtccggc
     8341 atgcgatgcg gacactcgat aattttccca tgaccgtcat ccaatgttga ccttaacctt
     8401 ttcaagaact tgctcgcaat atccgcattc ggcacattca cgcgaacagg aagaggtgcg
     8461 gtcaaaccaa tcaggcggaa atgccgcgtt atcgagggca gtcgggaaga acgcaggcga
     8521 aaagcccggc tcgaaaaggt cgagaagatt gccctcaaaa ccgccgcgct cgtaggcacc
     8581 gatcaccata tccggattcg catgctggcg cgtcgcgagt ttgacgacat cgacaagccc
     8641 ttcgaaacga tagaggtctt ctggacgcac ccacgtcgcc ttgaggatat gggggaagtt
     8701 tttcggatcc ttgtaaagcg tccagcagag gtgtggactc cagcccttca cgttctccac
     8761 attcatcgcc gcatcggagt gcgcaatgag attgtcatgg aaagtctgcc agggacagtt
     8821 cctcaagcac ccggagttcg caagtatgca gagcttcttg ccgttcacat gacaccaatc
     8881 ggagaaccga ctcaccgtat cgaggttacg ctgcacatcg cgtccgagat agtacgaatc
     8941 gaaaagaggc gcgaggtact tgaacgcctg tagcgtcgta agccgcatgt tcacactcgc
     9001 ccgcatctcg atttcgggga acaccttctt aagcgtacgc gcaacaaacg gcgaggcggt
     9061 cgtcacgatc tcgggcttgc agcgccacga tatgagctgc tcgagaatcg tgatgatgcg
     9121 ctcctcaaac tcatggctca tcgccttctc tccgtagcag ttggcgttga gaagaagatc
     9181 gagcttcatc ccggcttcac gaagtcggcc aaggtcgcgc tgtagcgctg cctcaagctc
     9241 cgcaggatca tcctcttcct cgtagccaag tttgggacgt cccgacggct catccaccca
     9301 cggaaagtag acctccttca ccgccggatg ccgcaaggca atctcggcga acggtgcgcc
     9361 atcctcgaaa tgctgatatc ctgccgcaag agcaatcatc gtctcattcc ttctctcgta
     9421 ttccgtagtc acgacgaaag aaacatttgc cttgtcctga aagatcgttt ctgcacctcg
     9481 atgcgcatgt ccagatcaat gacctctttc gcttccttgt ttcacgatcg gtgttagcac
     9541 catgttctta aacgaggtat tcgaattgtc gtcttggggg agaatcggcc cagacacgaa
     9601 ctcgttcccg tcgatggcct caggtgcccc agggaatcag caggttgcca tgatcctgac
     9661 accattgagc gtaaccgtga gatgtcgcct gtagagcgta cgaatccggc cacaaacagc
     9721 attgcacgtt gaacaccaag atgcccctgc ttttcataaa gtttccgttt ctcaggcacc
     9781 caattcccag ccgttgtgat actcggcaga gaggaagcgg ttcgcctcgt cggcataggc
     9841 gccgacgaat ctctcggact tcggatccca tttgagagac gaaagctgca tccgcgcgca
     9901 gatgttagcg atgtgtgcca tcgaaatcgt gcggtgtccc atctcgcaag tcgaggccac
     9961 gtcctgacta ccggtcagga caccgtccag aaagtcagac tcgtgaaact tcgagccgtc
    10021 gcgctcagcc gggggacggt gaagcttgat gtccttgtcg gtgagatcgg actccctgaa
    10081 attcttgagg cattccggca ccttgccctt tccggcgtag ctgccaacat cgcccttctc
    10141 acagtggaag atcgtctggc gcggccatcc ggcctccttg tcgatcttac gcacatggca
    10201 cctgacgccg ttcgcgaaaa cgaagtcgaa cttgaattcg ctcggccatg agaagatatc
    10261 gcgatccgtt ccggtcgcgt aatcggtctc cacgttctca accgccaccg gtcctgtgaa
    10321 ctgggtgccg aggccgcggt tgagctgatc gaattcgtgc gcaccgaaat cgggaatcat
    10381 accgccgcca tagcggctgt taaaacgcca gcaggtcgga tcgtgaatcg aggggatgta
    10441 ggcgttgtcc tcccagtgac gggcaggacc ctgccacatg ttccaacctg cctcggagag
    10501 ataggccgga cgcggcagtc gcgccgcaga acggccgtgc cccgcccagt ggtcgcaggc
    10561 cggaatgcag atcgtcgcgc tcttcacctt gccagcgtat ccgttaagga caatttcctg
    10621 ggtaaggcgg tacgcggggt tcgtcgaccc ctgattgccg acctgaaagg tgacgcctgt
    10681 ctccttcgcg acgcgtacca tcgtctggcc ctcggccagt gacaaagtca tcggcttttg
    10741 gcagaagacg tgcttgccgg ccttcatcgc ggcgatggac atgatcgcat gccagtgatc
    10801 cggcgtacag atgcagacgg cgtcaatcgc cgggtcgttg atgatcgtgc gccagtcgaa
    10861 cacgaccttg caggccttgt caccgtaatg ggcgttgacc acgtcgcgca gaacgcgcca
    10921 gccgcagacg tcccggcggt aacggccatc cgcccggacg aagtcctcga ctcccaagtt
    10981 cgtgttgcgg ctgccgtaga agtagtccgg tgcgatctct acgacatcgc agacggacgt
    11041 cacgcggcag cgcggatcct gcaggaagcc cggcgcgttc gtcgtcttcg cgatgaggcc
    11101 acagccgatg atcgccacat tgactttcgc gttcgccgcg atcttacgcg gtcccttgac
    11161 agtcgtgcaa ccgccgataa agatcgaggc cgacacccca ccgatgaaac ctcttctcgt
    11221 tatactcatg ttcattcctt tcaattaacg attacgacta aactcgaccg cgacgtaatc
    11281 ccgattccgt cgtcggcatc ctgaacacgt tcgaatcgat cctttttccc atcgaacacc
    11341 gccttacctg taacgcccga ttttctcaaa agggatgggc tggaaatgcg gaatcaactg
    11401 tcggcaggcc gcgttgacat ctcccggcgg cagttcgtta accacggcat tccattgatt
    11461 ggtgacccag ttgtcaccct tcttaaattc gcggcagtgc acgacgacgt tgtcttcgca
    11521 tctctgcata cggtggcgcc gcggaaaacc ctgcatgaca cctttgagtt ccggataacg
    11581 cgactggtag acggaacctt cgatgtcgac ctccctcttg agccgacgta tccaaccggg
    11641 ctccttgagc cagttattcc aaatttcgtc cgtaacccag cgcgaaccca gagcccgtcc
    11701 acaattgatg aagagacaat tacggacgac attcgaatag ccgccgttgc agtggatcgc
    11761 accgaacccc cgcttgtctt cgttacgaaa gacatcgcgg aagatacagc cgtcgatcgt
    11821 ccagcccgaa tgcccgtcgt cgagatagag agccgcctgc gccctgaact cggggttgcc
    11881 gatgttcgcg aaaagattgt ggcggaaaac gttgccgcgg caggagatgt tgcgtccaca
    11941 atgaaatgcg cccgaatcgt ccgtgccggt caccgcattg gaaatgacgt tgtattcgat
    12001 caggcagtcg tttccatcca cgttcaacgc ctcgtggatc gtgttgtaca tctcgttgtg
    12061 ccgcacgaca tcgccgacgc cccatgtctg gatggcccag gcatacgtca tccggatctt
    12121 cgagaaatcg tggatgagat tgttctccac cacattctcg ccgcggatga gattgcggcg
    12181 gtcgccgccc gagaccttca cgcccccggc gcccaagtca tacatcgtgc agtcggcgat
    12241 ccggcaatga tcggcttccg aaaactcaat gcccttgagc cgcgtgccgg aaatcgtcag
    12301 gttgcggatc acgacattcg tcaccgccga accccaaacc gcatttttcc atgcagggcc
    12361 gatcgtcaga tcgcagatcg tcacgttcgc aacaccccgt agcgaaacga tgtcctcgtc
    12421 ctgccagcaa agccgcactt gcgactgcgc gtcaagggga tgccgcggta taaggtagac
    12481 cttgttcgcc tcgacatcga catagcattc gccgggagta tccagttcgc agagcacgtt
    12541 gaggacgcgc caacggcggg gccgattgct ccaactgttg attccgccaa ggccgtaaaa
    12601 atggaacccg ccgagggtaa tcgtcttctc cttgtcgtcg atcgaggcaa tcggcatttc
    12661 atcggcacgc cattcggtct tccagaaccc gaagacatag gcatacttct cgttcaccca
    12721 gcggtcggga cggagccccc ggtagacgaa ggttccgcag tcgacgccgt tggttccggg
    12781 attgacgacc ttatcgaagg tcgcccagcc gtcctcccag tcgtccagtt cgccactcac
    12841 cggatccggc catcccgccg gcatcatccg cttgccgtcc acgtagagtt ccggaaccgg
    12901 atacggatgg accgtctccg tcggcttggc cggccaattc gcctgccagt ccgaaacgtc
    12961 cgcaaccaga acgcccgggc gttcgggatc gggcttgaag acgtctgccg gtatcgcgcg
    13021 tccgccgata agtctcgttc ctccgcacgc cgcaccccgc cagatgatcg gcgcatcggg
    13081 cgttccagag tcctgcggtt caagcgcgag cccttcctcc atctcgaaga cgccgcgcgc
    13141 gagccgcacc tccacaccct gtacacgttg ggcaacactc agagcacgaa tttgatcgcg
    13201 aatgacagtc aacttcgttc cggccggtac gtctatcaca accggcgtcc cggcaaccgc
    13261 acaccctgtc gccagcaaag tgaacaatgc gaacgacgtt atcttcctcg tcggcatacg
    13321 atccatctcg tcgcgctcct tatttgtaca taatcaacag tccctgcacc gaatacttaa
    13381 tcgcgagctc cgtcggcgac gtctgacaca agacgaactt acccttcgga tccaacgcca
    13441 gtccgtcgat gccttccggc aggaagagtc cgccagcggc acgcgcaatg atgaccgtgt
    13501 tacgcggcaa agccgccaca tttgacacaa tcggcatcgc gcctgcggac agtgtcagcg
    13561 atccgctggc gtttaagacc gtaccggcgg cgatatcgac agcagggatc gtccacgtac
    13621 ccgtcaccgt caggttgccg ttggtaacag tcggaagccc ttcaattgac gacactgccg
    13681 tcgtcaatcc ctctgcattg agatctaaaa ccgtattcgg cgcaaacttc atcgccaaga
    13741 agtctatccg gttctcgagt gaactgacct ccagacgatt cgttgtcacg acaaaaagcg
    13801 agccatcgcc cggatcgacc ggcgacacgt agtcccggta gttgtgtgac ccccgtcctt
    13861 gccggtcgat ggcaaaaccg aacttgctct tccactcgcc accgttattg ccgacgtagc
    13921 ttgtgaaagg ttcatcatcg aggtattgca taatgctcgc agcgatagca ccagaatatc
    13981 cgccgagttt gcgataacgg aattcaaagg cgtgcgcccc gggcgtcatc accacgtttc
    14041 ccgtcctgca ggttttacgg acgttctgat tgaccttgag ttcaccgtcg atgaacagct
    14101 tggagtgcgc atccatgcaa cacgcaaacg tccacgtttc gttggtcggg ctgtcattcc
    14161 aaacgtaccc acagtaagag agcatcttgt tttccgcgtc cccgtcctga tagtacagat
    14221 gcgcgttcgc gtcggtcgag acccgcaccc agagcgaggt cacaccctgc gacggagacg
    14281 tgagatagca aggctcagtc gcatcccagg ctacaccatt gtaaaggttg gtgtactcac
    14341 cgaggcccgc cacgcgcgaa tagtcgacat cggggtcggg atagcggaaa gcaccttgtt
    14401 gcacatacag cgaggcaata ccgttcaggt tcgtcgtgta agtgaaaagc gagccatcgc
    14461 ccggatcgac cggcgacacg tagtcccggt agttgtgcga tctccgcccc tgccgatcga
    14521 tggcaaaacc gaacttgctc ttccactcgc cgccgtcatt gccggcaaag cctgtgaaga
    14581 cctcatcatc gatggattgc ataatgttcg cagcgatagc gccggaattc ccgccatcgc
    14641 ggcgataacg aaactcaaag gcatgtgcac cgggcgtcat caccacattt cctgtcttgc
    14701 agtatccacg cgtgttctga tcgatcttga gttcaccgtc gatgaacagc ttggaattcg
    14761 cgtccatgca gcatatgaac gtccacgtct cgttcgtcgg gctgttattc cagatgtacc
    14821 cgcagtagga gagcatcttc atgtagctat ctccgtcctc atagtacagg ttcgcattcg
    14881 aatcggtcga gacacggatc caaagcgagg tcacgccctg cgacggagac gtgagatagc
    14941 cggggtccat acccgtccag gcggtctgat tgtagatgtt ggtgtactcg acgaggcccg
    15001 ccacgagcga gtagtcgacg ctgatgtcat atccgcccgt cggatcggga gacgtgtctg
    15061 cccgcacatt caaacccagc gtcagcgcac cgagcagagc cagtgtccct tttttcattt
    15121 tgatttacct ccttgtgttt tcagtaaatc cgcaccatca aatactgccc gaacgcggga
    15181 agcataccgt ccttaccgag cggcacggcc tgtcgccggg tgccgaacgg ctcctctgct
    15241 gtggctttcg cgcctgacgg cacaaccagc ctcgcacctt tgatctggac ctcgggatga
    15301 tagttgacga gatggataac aacaccgtcg ctgacttccc gcatgtcaac gaaaacctcc
    15361 tctggcaggt tctcgaacac aaaacggtta cgatagccga cccgaccaag ccccgccatg
    15421 aacgcccggc ccccatcttt cggcggcaac acgcgcgtga tccagccgag tgcgtttttc
    15481 tgcgcgacgt caatgtcgtt gcgccagtcg acgttgggga gcttgcggac gcaatcaagc
    15541 ggattcgtga aacgctcggc tccgttttcg tcgcgtagcc ccgtgtcgcg tcctgtgaca
    15601 acgagcctac cgcctttttt cgcccaatcc accactgcgg agacctgagc atcgctcatc
    15661 cacttcgagt tcggcaacac cagcacctca cagtcagccg ggatagacgg tgcttcagca
    15721 cccttgacga taacgtagcc gtacggcata tggtggcgca gcaggatctc gtccacagtc
    15781 gaaaccgcca gattcatcga gaccgcgagt ccgcaggcag cagacggata gaacaccctg
    15841 accggcagat agggcttgcc ggtaaaggcg gcacggttcg cctttacgaa agcgtcgaat
    15901 tcggcaagca cttttttgcg catcgcaaaa cgctcttcgt caatgaaacc ctcgcggcgc
    15961 acaggcgaca tgacggtgcg atccgtcgga atgccgcccc aaacaagatc ttcgacaagc
    16021 ggcctcaagt acatcgactc atcgagggcg tcgctcccgg cggcaccgtc actgagagcg
    16081 acgatctgct tgccgaggtc acgcgccatg cgcagatccc gttgacggtt gacaatataa
    16141 tgctggccgg ggaacttcgg gtcatcgaga taagcaggga agttgagcgt ctgcatcatc
    16201 aggagatcca atcctccatc cgccatgtcg atgacatcga cacttcgcgc gtggtagtta
    16261 tcggtgctac ggaaagatcc cgaattggcc gaaacaatcg catccggctt gagcgacttg
    16321 atgtggcggt agaagcgttt catcaccgca ttgacacact gtacgcgcca gcggtaatac
    16381 tcctgggcaa ccggatcata ctcgtccccg ttctcggggc ggaacgcttg cggaatacgc
    16441 agtcccggca tttcggcaaa gccgaaacgt tcctcaagat cgggcaggga atgaatgtga
    16501 cgtctgaacg cctcttggca ccgcggacaa tggcaaccaa agaagaaggc attgtcgaac
    16561 atcacgccgt cgaaaccgcc ttcggtcatg gcgatcgtaa ggattttctc cgtatattcg
    16621 acccacttct cattgactat gcaggggagc cagcgggccg cttgcctact gtagatcatc
    16681 ttcattccgc gctcatcaat cgccgcccag ctttcgatgt ccggaatctc cttctgcatc
    16741 acctcatggt agagtgtcgc gaactgcacg taagcaagta cggtgacacc gtgcttatga
    16801 caattatcga caaacttctt cacattcggg aaatcctttt tctcaacctc ccagcccatg
    16861 cctttgtaga agcgggagtg gagagcgttg agtccgatct cctccatccg cgccggcgcg
    16921 tgctcgtccc accagtcgag ccagtctttc acccactttg agcctccgta gataccaccg
    16981 gtggacctcc tgccaatgcg gcggtacatc gtgaaaggct cgtgcgcgcc gaaggtccag
    17041 cgcgtcgtga tcggatcgac cgccacatcc cgtgtgatcg tggacgcctc cagacgcatc
    17101 gacaacgcga aaagaatgcc gcatacaaaa ggaatcacac atctgttcat ctcatctctc
    17161 cataccgagt catctgcctg cctttccgga ataaagtgca ccggaatcgg cgaacgacag
    17221 atactcgcgc gccttgagtg aatcgagaaa tgattttggc tcattgcggt attcaacccc
    17281 tacgaatgcc gtcggatccg cagccagcgc gaatttcgca atggtaacgt aacggccttc
    17341 ggcaacaagc atcggtttca cgcccgtccg cttctccagt tctgcagcca gcgtgaggat
    17401 ttctgctgcg cgcaaggcga cgagagaacg tcccgcgata tagagatcca cggccaccac
    17461 ctcgtcgtca ccgcgccttt cgtagtacga tcgtttacct ttccgctccg aacggatgac
    17521 atagcggcct gcgtcggcaa atagcgtggc cgtcacttcg cccgcaccgg ccagcccacc
    17581 caaacgcaag gtcgcacgcc tatccttggc cagttctcct tttagcgttg caagaacacc
    17641 gctgcaatcc ttaccgatga gttctttcgc cacaagcggt tttgcgttct gcgcggcgtt
    17701 atcgaccgac acgccgcgcg cgacgaactt ctcggcgatc gtatcgtaaa tcgacttaaa
    17761 gccaacgaga tgcgagactt cctcgtcagg agttacagtg cagtcatccg ccgtgaactc
    17821 ggtcgtctcg gtctcagcga cctgccttga ttcacccgtg agatgcttca gtaaaaagct
    17881 cacagaacgt tcacgatggg tcttggacat tttatgcggc cccggtgcct ctgaaagctc
    17941 gtaccaatcg gcaggcagac cgactttcgc cgccacttct ttcaccaacc gatgcgtcga
    18001 acgcgagccc tcgatctgaa agaagtcctc gaccgcagca ttgatgagaa cgggacactc
    18061 tgcggcaaac atgagtgccg cgtgattgaa gccccaagaa aacgcgtcaa agaaattctg
    18121 ttccgcgtcc tgcggaccgc acgtcagcag atgtttgcgc gcggaagaga ggtagcaaga
    18181 cggagtcgcc gccttcacgc gatcatcgac ggccataaag aacgccgcca tcgtcccccc
    18241 gccggaatta ccgcacacac cgagcttgtt ccggtcaatg tcgggacggc tctcgaaata
    18301 atcgaagacg cggacggcgt cccgcaacat atacgtcgcc gtcgtctcgc ccaacaacac
    18361 cgcatacgca ccgatgcgaa cgtgctcgtc tgcgctcctc aaacccgctc cctgtgaacg
    18421 ctcgccctgt ccgagtgggt cataggtaat caccgcaagc ccattccgtg cgcccagttc
    18481 acaagtatga agataatcct tcccgccttt accgcggtcg ttatggccgg gaatgaagat
    18541 gaacccggcg tagggcggcg caaacttctt ggcatccgga aggaagacga gtgtcgcaat
    18601 gaattcgccg ggagcacttt ccatcagcac cttctcgatg cggaaggcgt cgaagaccct
    18661 tacacctgtc gttttcgctt gaagcggcgt gcgctgaatc tcgtcatagc cgatcacacg
    18721 ccggaaatcc gttttgaact ccgtgcgctt cgccacaaac gccgtcttat ccggaacagc
    18781 cagccactcg gcatcgaccg ccgcatcccg tgcggccaga gacgcctcca gcgcatccac
    18841 ggtcggggag gcggcatgat acgtgttgca caccggcccc ggcgcggcta agagccgtcc
    18901 gggcaggtag aacgccggac gcgccgcctc cagacgcatc gacgaggcga aaagaatacc
    18961 acatgccaaa agaatcatac gtccatccat ctcatctcgc catgccgttt ccgaaaccga
    19021 taggaaatag tatacatcgt tcaactgcag ttcgtccaat ggattcctcc cgctgtaggt
    19081 aatcgatcgg ttggtgacag ggcgtaagcg agacgagtgc gtgttttgtg tgtgggcgca
    19141 agctaggcga acgtatttgc ggcggcaagc ggcattccgc gcgacgccgc tttaaagcac
    19201 cgggaggaac gagcgtctgc tcgtccggcg acggtcacgc cgccattttt acactcgtcg
    19261 ttgcgaacca ctgcgtggtc gcaacattcg atcccgatag cgatcccgat cccgatttgg
    19321 atgaaaccgc gaaaccttga aaccaaagag agttgaaggt tcaccttgca ctcttgccca
    19381 gcgtcacggg cccacgcaaa tcgccgcgat cgcattctta actatgctat tcatcatgtg
    19441 atcctcccct tgtcatgatt ttcatgcgat ctatttgacg acaatcatca cgtacatccc
    19501 tagtgtcggc agtacgccat ccccttcgag tgtcactttc cggggttcat tcccgaacgg
    19561 ctcctcaaga acggcggaca cgcctttcgg cagagcaatt cgtgcaccga caatccgctc
    19621 ctctggctta tagttgacca aatggacaac caagccacca gacacaagcc gcctgtattc
    19681 cgcgaagacg tacggcggca gattatcgaa ggacaccggc ggacgccaac cgacctttcg
    19741 gagatcctcc atcagcttct ttcccccatc taccggtggg ggaacggtat agccccaccc
    19801 cattaccgca ccacggatga gatccgcttc gacgcggcag accacattcg caaatccttg
    19861 cagttgcggc aggagcggat tggcccgacg ctgtgcgatc cagtcgtcat accgtccggc
    19921 atcaccggtc accacaagtt tcccccctga cttcgcatat gccacaagtg cctcgacctg
    19981 agcatcggaa agcgcgacaa ggccaggcac aatcaccacc tccgtctccg gcggaaccgt
    20041 catcacgtta tccggcgaag aaacgagata cccgtacggg acacgatttc gtaacaggat
    20101 ttcctcggct gcaacgactc cctgatgatt cctctccgac agatagatct cgcgttccgg
    20161 atagaaaatg cgaacgctgt ggatggccgg tgcggtgagt tccgccgcat gcgctgtcgc
    20221 aaaggcattg aaacgcgtgt gtatcggctt tcgagcttca aacaccttac gattgataaa
    20281 ccctggctcc ggcgacggga agattgatgt gcgatcggtg gggataccgc caaagacgag
    20341 atcctccacc attggcagga ggaacttcga ttcgcgatcg gcatgcagat gctccacgtt
    20401 gtcgcacaaa gccacgatac gttgccccat atcctgtgcg aacttgagat cgcgcacgcg
    20461 attgcgaatg acacctccct cccgaacctc cggaaagttg gcattctgca tgatgataca
    20521 atcaaacgca cggcacagct cggccatgtt ctgcgcttta gacaaagcac cagcgcggtt
    20581 gcgatagggc gacggattcc cagcgacaat ggcatcaggc ttcacatgct tgatgtggtc
    20641 acgtagacga gtcatcacac ccgtcatgac atccgcccgc cagcgaaacc attcctgcgc
    20701 tacaggatcc ttcacttcaa gagtggtttc taccgaaata cggatcttcg gcaacatcat
    20761 gtaatcgaga tcgtcgaaac cgaaacgcgc ctcacgatcc ggaaccgtct tgagatgttc
    20821 gcgaaaagcc ttctcgcacc gctgacaata gcacgggaca tcgtagacgt tgtcgaacat
    20881 aatgccgtca aatccgcctt ccacgagggc gatcgaacac atccgcttaa gatagtcctc
    20941 ccactcgcga cagttaaggc acggcatcca acggaaatac atgcccccgt ataagttgtg
    21001 gccgccggtt tccgaaacgc ccgcccagga gtctatctgg ggattttcac gacgcatcgc
    21061 ctccggatag agcgtgccga actgcacgta ggcgagtgcc cgcacgccat gcgcgtgaca
    21121 gttcgccacg aacttcttga cgttcgggaa atcctttttt tcaacctccc agcccatacc
    21181 cttgtagaag cggctgtgca ggaaattgaa gcccatttcc tccattttct ccggcccgtg
    21241 cagatcaaac caatcaagcc agtcttgtac ccacaacgca ttgccgtcga tcgctcccgt
    21301 acagatacgc cccacccgac ggtacatggc ataaggctca tgcgctccga acgtccagcg
    21361 cgttgtgatc gggtcgattg cagcatcccg tgcgatcgga gatgcctcca gacgcatcga
    21421 caacgcgaaa agaatgccgc atacaaaagg aatcacac
//
LOCUS       DAOSLE010000030        21149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2748_length_21149_cov_7.936522, whole genome shotgun sequence.
ACCESSION   DAOSLE010000030 DAOSLE010000000
VERSION     DAOSLE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21149
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_21149_cov_7.936522"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            611..1168
                     /locus_tag="PK576_03665"
     CDS             611..1168
                     /locus_tag="PK576_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOE60719.1"
                     /translation="MSKKIYISANAYLLDSFRLARRILDSGWLPDDLIVLWRGGAPVG
                     MAIHEFLSYHGLRPRHRTLKCYSYTGIRTHREEVAFENADAVFSSVTPGSRVLVVDDV
                     FDTGNTARAVLDRLAPSGAETRLATVYWKPNRNQTSGKPDFYLHETDEWIVFPHELEG
                     LTPAEVHRKDPALHALLKSPASQPR"
     gene            complement(1156..2340)
                     /locus_tag="PK576_03670"
     CDS             complement(1156..2340)
                     /locus_tag="PK576_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOE60720.1"
                     /translation="MAKMFTAAPRVVVILETCVQPDREKLNGILRYVRFYGPWHLHII
                     QNRVGEQRLAHFGAWHADGVIVGQSMLDMERTLARAKMPIVLIDPLPRHLQPDSPFRH
                     CCRIQNDAVSVGRAAAEFFLERGFERFAYVGEVMGRLWSKGRGEGFAGRLREVGRVCQ
                     VYAPPSRAEIRDWAVEQLRLAEWLASLPKPVALFAAMDIRARQVLDTCLITGIKVPDE
                     VAILGVDNDEQLCLGSFPTLSSIECDMENCGFMAARLLNEQMRGRHRKRVELTYGVKR
                     IEERHSTRQVPHMEDRLVARALEFIRLNACEGIGVRDVVAYLKVSRRLAELRFRTTCG
                     HSILDAIRAVRLDRVRRLVAETDLPVSEITSRSGYQTDDHLRRLFKREFGLTMREYRQ
                     RG"
     gene            2524..7419
                     /locus_tag="PK576_03675"
     CDS             2524..7419
                     /locus_tag="PK576_03675"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOE60721.1"
                     /translation="MTRYRQSVFLFLLPFCVVAANTPTPDLVLARKGRPADRAIVCPV
                     QASPSQRYAAEELQTFVEQMTGVRLPITDDRAPLPAKAILLGDTRHTAAVLGATPALD
                     GLGTDGFLLKTVPPHLVIAGSPVRGTLYGVYELLERYGGCRWYTADYSVIPKRDTFAI
                     PPLNDRQTPAFAIREDRWTDMIPGDVAARNRCNGHDMLLEERHGGKVRFGAGLVTHTF
                     YSLMPPREFFAEHPEYYSLINGKRRAENAQLCLTNPDVLKITIARLIERIRKDPNAQI
                     YSVSQEDNRAYCRCAACEAINQREGTPAGSLIAFLNQVAEAVEKVYPDVMIDTLAYQY
                     TRTPPKTLKLRKNILIRLCTIEFDFSLPVNQSTKASNKRFIDEIRGWQALTDNLYIWD
                     YVTNFGSYVGVYPNFGALQGNLRFYRDHGAVAIKQQGARPGKHADFAELKAWVIAKLL
                     WNPDQPLEPLVTDFLEGYYGAAAPHVRAYLDATTRLVADPDIHIGCFNRPETYPWLTD
                     DLLAKFADTFRQAEQAVRNDPPRLHHVRMLGLGVLYTQLRRIPLPAAPQIIWAADGAR
                     LNNIPSATEALAREVVARLDEEPGILLLQNSLTHHVIQAQYRALAGRVPHHTFSNDAI
                     TASLTELENAKLGWLAKTGDPAFNYLSPNRDWGVQIQPDQQERTAAELFGMVYRKAKT
                     SQDAPRFALSRLGAISSERHLKTEGSRLLFSARFRNTRQTLQQETEPMIRAALNLGQH
                     GRFCWRTAPDAPWRTQSELREPVVALSAAERGTGQIEIASPVTRRMLRLTFDPARAVS
                     VTLRLHQAEAESVRVQITEKPETLAPGAERVCPLTLEIADPPPDLPGSLATAFVTPPV
                     GQPPHIVWQGQPAARIVVPVRATPVERSAARELTLHLEAICGAAFETVTEDEPKTGPR
                     LLVGNTDAARRLIPGFDPASLAYDGILLKTAGDDLILAGHPQRGPIYAVHTFLEDSVG
                     VRWWTSDETFLPQHTSLPLPTLDLRYEPKLKFRETYYLDSFNARFKVRMKGNFSSRTR
                     FMLDPLEMIPEAYGGNHRLIHFAGRNSAYHSFYELLPPAKYFKAHPEWYSEINGKRTH
                     TRAQLCLTNEEMRRELTKNALALLRTDPGADFIQISQNDHAGRCTCARCLAVEKEEGG
                     VGTASGPLLRFVNQVAEAIEKEFPNVWIDTFAYQYTRKAPTKTRPRRNVLVRLCDIEC
                     SFLHPIEGTPQNAKFLTDLVEWSRIAPGNLFLWDYVTSFSSYILPHPNLRVLAPNVRT
                     FVKHGAIGLFEQGDALCRAGDFVRLRQWVLAHLLWNPDLDADALTDTFLAGYYGPRTG
                     AALRRYLDFLHDRAARADVYLGCYQNNVTHWLDAEGILEALRLMDAALAEAKAEEQAD
                     PVGRKGLADKIRREKLPMDHVVILNRPLLQRHAKATGKPFPVTADPLAAVEAWIAACE
                     RFGVKAHRETTTADTFIDYKKSLLERARMASQKVSPPPCCATLPPASWHDVQEFEFDQ
                     RPAGQVAFVEDKAASNKMAAQMPGNHKIWAVQFPLTSFIPLSGTASGTPRCTLHVYAR
                     ADATATEGNGLALGVYDFTARKTVAHQWIPLSELTGPGYKMFKFGPLPITEGCKFWAN
                     PPERTGEVQHVYVDRVVVVVE"
     gene            7472..8884
                     /locus_tag="PK576_03680"
     CDS             7472..8884
                     /locus_tag="PK576_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60722.1"
                     /translation="MKKMVFIVTAGLSACLLHAASWVPTQPSPDEAAIACINTSVTNG
                     TDVDITATLASNTAEVVIGTALSDRHTFLRAVDLSGRTGTGLGSADNSAFHAAGVDDA
                     ATGAAILLKRLSGDVHGAGVTHAFGLLAWSDGALGNTPATLQIDHLTGTVSANVHPDG
                     TAAALYARTALHLNLMPGALLFGGSYGWDLGDQTLTNAQAELVGLLAKVREGAATASE
                     SNTLKTAASGYAILGGEEDDFLRFGGLATLFGQVDLGDGMDVVSLCDLPSNTVTRLPA
                     IANAELLLITNSPIFSASLAGAPKETVIAGDSFCTFVDELTTLDSLSGTGTLASEGTI
                     WVTNLLSVSGFLAAPTGSALIIEGNLGLADGLACSLRACGTADGWTNDRITVTGTVTV
                     DGGGTVDLGCTPSDPIPEKERRILMTVAGGVINSANLASWTLTGTGWDEDPSLIRRIG
                     TDGVTIYASVLKGGTLLLMR"
     gene            8908..11211
                     /locus_tag="PK576_03685"
     CDS             8908..11211
                     /locus_tag="PK576_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOE60723.1"
                     /translation="MKNLSKPLAIFSMTACLAGTVHAQQILTTVQNITAAPAPAITWA
                     TQPDGVYSLLASDTEGGPFDTVLIASTQTVENSITYVDWRQATEGTRRYYRVVENTPG
                     YATYMIVDLSGGPDATVYPVSYTNAVPDLLTDSAYKTNKMVFRLIPGGSNITGFPEDV
                     PRGQYTPYIPLGTMTLTRPHYMGVFEVTQDQYALVMNTNPSPSNRLGATRPVQDVTYN
                     DIRGSAAQGGGGWPSNSEVYTNSFMGRLRRKTGYEEFDLPTAGQFEFATRAGTTALFY
                     NGFNDPTNTTDFSGITNIARCCGNQLSTWGGYTYAASVGSYLPNAWGLYDMTGNLSEH
                     CLDWGGRNSGTDPVGPQSGTSRTVRGGAHSTSALSTLYAGARDSMNPGKVTQSGVFGF
                     RTALRLPDTPSEEMRSSVSAGTILSVTGTPRPASRITWQTVPGKTYIVLCATNETGPF
                     ETQIGDTLTASGNTLSVTDTGYTPDGPQRHYRVEELPGGYARYRIVDISGGTNAASWP
                     VTHTNVLPDINSALYRTTRIAFRLIPPGSFTMGAPLSTVGATTKTTPHKVTLTRPFYM
                     AVFETTEAQHSLIYGDGSGTSQYPVGNVTYDMLRGSTSDWPLSSNVAPDSIIGMLRAK
                     TGGALDFDLPTEAQWEYACRAGTTSAFNNGDDLKTETDFGTLKATLGLNNRHAGGAYQ
                     PNSWGLYDMHGNLLEWVLDRWQEDLGTADVVDPNGPSTGTKFSTRGSAYMAKDSFKYA
                     SFSRENGGMNRYGSTGLVSYRLACRLD"
     gene            11239..13122
                     /locus_tag="PK576_03690"
     CDS             11239..13122
                     /locus_tag="PK576_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase"
                     /protein_id="HOE60724.1"
                     /translation="MNAHMKILTVSLTLALLWGFSGCRPPETARTPRDQALDFIRNET
                     QFHLGYLPTEQSHPKTRGLSQALQADTAKGIRMLLSVDEDIPPVARRVAASPEFAELR
                     TAIKTALDANKKVYFSGCGATGRLSILLDAANRRFWRETYERHPDLKAVCGDMAGRTH
                     AVMTGGDYALVRSVEMFEDYITFGYHQMEQAGAAEGDVIVAISEGGETSSVIGTILRG
                     LDVKAKVFFLFNNPADLLADKLERCRRVIRNDAVTKLVLCTGPMGVAGSTRMQATTIE
                     MLVAGMAYEAGLKEHLQTKLNSTQIASLGLDARTPEKTLEQFDTLLGQIRSERNVTAL
                     ARLADYEESLYTRGGRITYFADGYLLDIFTDTTERAPTFKIPPFRRCDDTLSPAPWAF
                     VKDPLRTTPEAWLRVLEHEPRCLDWSPETYIRLGASPDIRKNPPRIDRASLHTFLIGN
                     EPDASRTEIRPNAAMAVLIGDEAAALAPAANAPWRKAFLDAAKPFDVRAALVIGKQAV
                     ANGDDTLIHVDVDIPETPLNLFSHLAVKLTLNNTSTASMGKMGRLTSNWMAHVEATNK
                     KLLDRSTRLIVELAGVDYETACIALFESLEEMKTWDEARKKTVSPAAYTVKRLTARAK
                     PGK"
     gene            13146..15176
                     /locus_tag="PK576_03695"
     CDS             13146..15176
                     /locus_tag="PK576_03695"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOE60725.1"
                     /translation="MIRSALPHLLQTLLLAALTAAPSLSLCAEDVAVSPPLDWTLGAG
                     DLKGPLTFFGAETMRTTNVVWQGRDALTATWTGHPEYGNAFTVTVTWQRAPDGLWSGV
                     LAYSGYRGSRFIEEIHFPRLSTACDTHAAFLFGGNHSGRLYAVGSGPLKPGSRFRRDY
                     CGGMQFSALLRTNGTSLYLDHRDPAVSVKECEIAIRADGRRFTYTGIHIPGLPEKPAA
                     DYRIPYPSCHTLFQGGWFEAGQLYKKWGTAQSWHASRQGVNPMRKIGIWVWNRGLIKD
                     VLPPVERLQKELGAIPVALDWYWWHNNPYDTDYPDFWPPREGVEPFRAAVARLKRQGI
                     YSQVYVNGVCWDMDGKTWHQGGAQDVVVLRNGQPRNTAFNKYNLHRLAYMCGEAPHFQ
                     DKIASLMRHLCDSGLDGQYLDMIGNSTMRYRCYNPLHRHPKGGGTYGKDGFRALLERI
                     RRENPNYPLTTEGCNEAYMDLMDGSIVCNSTGLDRLDALPLFMSVYHGRYAFFGNYAY
                     PDGTRPWDPLWPPEDRWKEEKPWHRLYPDQFFAELARTVSWGCQPMVCNIKETLFTDP
                     DLVPNLRFILDSARFYHANLDVLFDGQMLSPAGFTCATAGVDYLIRSIFTKEHECKPQ
                     HAKLPAVLHSYWQAPDGKKALILANWTRASQPWSFNGLSGVIPARSYARIAP"
     gene            15187..16194
                     /locus_tag="PK576_03700"
     CDS             15187..16194
                     /locus_tag="PK576_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60726.1"
                     /translation="MDIEFHYYITHLIAARAGVRGEALRIIPHACQLTDDNDQVYTIR
                     NGRQGIYRNRISQTFQPLNSHRERLAVYPLFHFIPGDPHAPGSARVDGQTHPFNTTPN
                     SPLANRILDATLATHDPYQIGIACHAYADTWAHQNFVGFKCDFNRFDDFVARLIPNIG
                     HADVQTRPDQPRLVWRDERLMVPVVNNKQRFLDAAAALFAKLRAWAAPQHPDSESEHR
                     ALRTDLDTAIGTDPGEETADAARLAGYRQLAEHPAYGATPIPEYAPDAWFRSAVRVIR
                     QRGGNPGPGRRMRKGYVFRTGYEFSDWYRFQNAVVSYAERTRALLCEIPALAEEYRRA
                     Y"
     gene            16733..17923
                     /locus_tag="PK576_03705"
     CDS             16733..17923
                     /locus_tag="PK576_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOE60727.1"
                     /translation="MTFLFRLLREIPPRLLGSFVIKAGLGNLRAIRRFEQRKKQGAVF
                     PPFLFISVTQRCDLACKGCWATGVEKPVDMSEALLHQIVTEGREQGCRFFGILGGEPL
                     LLPWLPDFFRRHRNLYFQLFTNGQRLDDALARKLRKAGNVSPLISIEGNALSYAARRG
                     AGTAYETALTAIDACRRQGLVTGVATSISTSNFNDVVNPAFLDDMVARGAHYVWYYLY
                     RPSGPEPGFGEALNDTQVREARRFLLEQRRRCRTAVIIDAYWDHEGNALCPAATGISH
                     HINARGDVEPCPPIQCADSRVRPDAPVAATIRDSPLLAAFRSEVPRLTRGCVLMDHPA
                     ELAALARRHQAYDSSGRGCFFEELAARPILGCHNHSGEPLPETSWAYRFAKKHWFFGF
                     GAYG"
     gene            complement(17937..20741)
                     /locus_tag="PK576_03710"
     CDS             complement(17937..20741)
                     /locus_tag="PK576_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60728.1"
                     /translation="MCFAKRLGRLLCCVEGVFIVCGMALGAEIHVSPKGSDEAPGSVR
                     EPVLTLEKARLLAREARARQPGEPVSLLLHPGTHRVTRTVAFTAEDAGTREAPLQLLA
                     WRTPSTPPEARPVLVGGKVVTGWKACSFNGRDGVFEADLKPLGITTPFRQVYLNGQRL
                     IWARYPNADPALPYSGGWAYVDGVRPPMYKDIEGERTDTVVLRERDVHAWSRPEEGEM
                     CIFPRYNWWNRIEKIAAFDPGSRTFTLAKKMPYAARPEDRYCVMGLREELDAPGEWFQ
                     DVAGQRLYLIPPAGADLTRDQITVPTVNDILSFNKTHHVRIANLELTCAETHALRFQD
                     CDHMTVEACRIHDLGFFSGSGIGIRGGREGRIRGCDIWHIGGHGVSVYAGDVDSMTRC
                     FHEVENCYIHHVGQFNRHGLGVMIGGAGVRVAHNLMHDLPRSGIFHGGVLHTLEYNRI
                     RHCNLEMEDTGCTYTGGWCGGWHTIRYNHCSDSIGFNNHGKFFVFAWGIYLDESGCGN
                     DVYGNLVERCQVGAMHLHNARENHIYNNIFANNAGPEGKTHQFSLQTWNNSPTAVFLR
                     DRQPKMLKAYTRLMANPEWAKMRGMHVSPADPFLPDGTIMRGNRIFRNIFLYSEQPDS
                     RYIRENGVNLTHNLINSNVVWNGGAQPVKTGKQAVRRPIADLTERIPNAAFARAVDAA
                     ALASDPNQTIAAEWFWYQKILPGLQAERVTRGDAQALRLFGAFNGERKYIKYPCVRSA
                     PFTLPYGKHYRLTFALRHHEADGEVLVRLVCENKGLWKALGPRGFLKRSDMSDISTAS
                     EALPCETGFYLPKPGEAGFDARMEAFTLHFEFQSKQGWAEISGLRLEEVEPASEWEAW
                     QMAGADRDSVVADPQFVDAARGDFRLKPDSPALKLGFEPIAFDKIGPYPDAARATWPI
                     IEAEGVREHPEWLTSVPIPK"
BASE COUNT         4129 a         7258 c         6020 g         3742 t
ORIGIN      
        1 tcaaaggcgg cggcgtgaaa cgccgcccgg acgagcagac gctcgtccct cccgttgacc
       61 cgcgactggc gacccgcgac ctgcggtgtc gggaatgcgg gcatccccgc ccgtcggtag
      121 ggcggtttcc ccgaaaccgc cgcctcgcaa gcaaacaaac gtcgcgggtc gaatgtcgcg
      181 ggtcgcgggt caacgtacac gaaaacgtgt acgattccga tagcgatccc gatagcgata
      241 gcgatttgga tgaaaccctg aaaccatgaa accatgaaac caaagaaagt gtcccgtggt
      301 taaatgatcg atcccgatag cgatcccgac cccgatcccg atttggatga aaccatgaaa
      361 ccagaaacct tgaaaccctg aaaccaaaga gagttgaagg ttcaactgct ctttttaggg
      421 taaaaaaagc gttcccgtct tgcgcgggga gaaacacggc ttcggcacgc cgggctccgc
      481 gccgatggcg cgtagctgcg gacgcccggg gggatggttt tgatctcccc aaaccatgcc
      541 gattaccgta accttattca tcgctgtccc ccttttgtgt ttatgccttt gagcgcgtaa
      601 aggaaaagcc atgagcaaaa aaatctatat ctctgccaac gcctacctgt tggacagttt
      661 ccgcctcgca cggcgtatcc tcgactccgg ctggctaccg gatgacctga tcgtgctctg
      721 gcggggcggg gctcccgtgg gtatggcaat ccacgaattc ctctcctacc acggtctgcg
      781 gccacgtcac cggacactga aatgctattc ctacaccggc atccgaaccc accgcgaaga
      841 ggtcgctttc gaaaacgccg acgcggtctt cagctccgtc accccgggtt cgcgggtgct
      901 ggttgtcgat gacgtgttcg ataccggcaa caccgcgcgc gccgtgctgg atcggctggc
      961 gccctctggc gcggagacgc ggctggcaac ggtttattgg aaacccaacc ggaaccagac
     1021 ctcgggtaaa cccgacttct atctgcatga gacggatgag tggattgttt tcccgcacga
     1081 gcttgaggga ctcacccccg cagaggtgca ccgcaaagac cccgcgctcc acgcgctgct
     1141 gaaatcgccc gcgtctcagc cgcgctgacg gtactcccgc atggtgaggc cgaactcgcg
     1201 cttgaacagg cgccgcaggt gatcgtcggt ctgatagccg ctccggctgg tgatctcgct
     1261 gaccggcagg tcggtctccg cgacaagacg ccggacccgg tcgagccgca ccgcgcggat
     1321 cgcgtcgaga atcgagtggc cgcaggttgt ccggaaccgt agctccgcca accgtcggga
     1381 gaccttgaga tacgcgacca cgtcccgcac accgatcccc tcgcaggcgt tgaggcggat
     1441 gaactcgagc gcgcgggcca ccagccggtc ctccatgtgc ggcacctgcc gcgtcgagtg
     1501 ccgctcttcg atccgcttga ccccgtaggt cagttccacc cgcttccggt gccgaccgcg
     1561 catctgctca ttcagcaacc gggccgccat gaaaccacag ttttccatgt cgcattcgat
     1621 gctggagagg gtcggaaaac tgccgaggca aagctgctca tcgttgtcta cccccaagat
     1681 cgccacctca tcgggaactt tgatgcctgt gatcagacag gtgtcgagca cctgccgcgc
     1741 gcggatgtcc atggcggcga agagcgcgac cggcttcggc agcgacgcca gccattccgc
     1801 cagccggagt tgttccacag cccagtcgcg aatctcggct cgcgacggcg gcgcgtagac
     1861 ctgacacaca cggccgacct cgcgcaaccg tcctgcgaaa ccttcgccgc gccccttgga
     1921 ccataaccgc cccatcactt cgccaacata cgcgaaccgt tcaaatccgc gttcgaggaa
     1981 aaactcggcc gcagccctgc ccacgctgac cgcgtcgttc tgaattcggc aacagtgccg
     2041 gaacggcgag tccggctgca gatgacgcgg caagggatcg atcagcacga tcggcatttt
     2101 ggcccgggcc agggttcgct ccatatccag catcgactgc cccacgatca cgccgtccgc
     2161 atgccacgcc ccgaaatgcg ccaaccgctg ttcgcccaca cggttctgga tgatgtgcag
     2221 atgccacggc ccgtaaaaac ggacataccg gagaatcccg ttcaacttct cccggtccgg
     2281 ctgcacgcag gtctcgagaa tcacgaccac ccgcggggcc gcagtgaaca ttttcgccat
     2341 gtcgtcccct tccttccgta ccatgaagta tgcgacgaca acgcgcatcg tgcaagcgcg
     2401 ttttgcgcaa aatgccggat caggttatgc gcaaaccgtg gtgttcattt cgcgtccgga
     2461 cctgtatact gttttttgag tagagaaatg ccaatccacg acccctgacc ggaaaggaac
     2521 ctcatgaccc gctaccgcca gtccgttttc ctgtttctcc tgccgttctg cgttgtcgcc
     2581 gcgaatacgc cgactccgga cctcgtgctg gcccgcaagg gacggcccgc cgaccgcgcc
     2641 atcgtgtgcc cggttcaggc atcaccctcc caacggtatg cggccgaaga actacagacc
     2701 tttgtggagc agatgaccgg cgtccggctg ccgatcacgg atgatcgcgc accgctgccc
     2761 gccaaggcga tcctgctcgg cgacacgcgc cacaccgccg ccgtgctggg cgcaacgccc
     2821 gcgctggacg gcctcggcac agacgggttt ctcttgaaga ccgtcccccc gcatctcgtc
     2881 attgcgggga gtccggtgcg aggcacgctc tacggggtct atgagctgct cgaacgttac
     2941 ggcggatgcc gctggtacac cgcagactac agtgtcattc cgaaacgcga cacctttgcc
     3001 atccccccgc tgaatgaccg ccagacaccc gccttcgcga tccgcgagga ccgctggacc
     3061 gacatgatcc ccggcgacgt cgcggcccgc aatcgctgca acggccacga catgctgctt
     3121 gaggaacgcc acggcggcaa ggtgcgcttc ggcgcgggtc tggtcaccca caccttttac
     3181 tcgctcatgc cgccccgcga attctttgcg gagcacccgg aatactacag tctgatcaac
     3241 ggcaaacgcc gcgcagagaa cgcccagctc tgcctgacga atccggatgt cctgaagatc
     3301 accatcgcac gcctgatcga gcgcatccgc aaagatccca acgcgcagat ctacagcgtc
     3361 agccaggagg acaatcgggc gtattgccgc tgcgccgcct gcgaggcgat caaccagcgc
     3421 gagggcaccc cggcgggctc gctcatcgcg tttctcaatc aagtcgccga agcggtggag
     3481 aaggtctatc cggatgtgat gatcgatacc ctcgcctacc agtatacccg caccccgcct
     3541 aaaacgctca aactgcgtaa aaacattctg atccgtctct gcaccatcga gttcgacttc
     3601 tccttgccgg ttaaccagag caccaaagca tcgaacaagc ggttcatcga cgagatccgc
     3661 ggctggcagg cgctcaccga caatctctac atctgggact atgtcaccaa tttcggcagt
     3721 tatgtggggg tctaccccaa cttcggcgcc ctgcagggca acctccgctt ctaccgcgac
     3781 cacggcgcgg tggccatcaa acagcagggt gcgcgacccg gcaagcacgc ggacttcgcc
     3841 gagttgaagg catgggtcat cgccaaactg ctctggaacc ctgaccagcc gctcgaaccg
     3901 ctggtcaccg attttctgga gggatactac ggggcggccg cgccccatgt ccgcgcctac
     3961 ctcgacgcga cgacccgtct ggttgccgat ccggacattc acattggctg tttcaacaga
     4021 cccgagacat atccttggct caccgatgac ctgctggcaa aattcgcgga caccttccgc
     4081 caggcggagc aggccgtacg caatgatccg ccgcgcctgc accacgtccg gatgctgggt
     4141 ctcggggtcc tctatacgca gctccgccgc attcccctgc ccgccgcacc gcaaatcatc
     4201 tgggccgccg acggcgcgcg cctgaacaac atcccgtccg ccaccgaagc gcttgcccgc
     4261 gaggtggtcg cccgtctcga cgaggagccg ggcatcctgc tccttcaaaa cagcctgacg
     4321 catcatgtga tccaggcgca ataccgggct ctggccggcc gcgtgccgca ccacacgttt
     4381 agcaacgacg ccatcaccgc ctcgctgacc gagctggaga acgccaagct gggctggctc
     4441 gcaaaaaccg gcgaccccgc gttcaactac ctctccccga accgggactg gggcgtgcag
     4501 atccagcccg accagcagga acgaacagcc gccgagcttt tcgggatggt ttacaggaag
     4561 gcgaaaacct ctcaggatgc cccgaggttc gccctctccc gactcggcgc gatctccagc
     4621 gagcgccatc tcaaaacgga aggctcccgc ctgctattct ccgcacgctt ccgcaacacg
     4681 cggcagaccc tgcaacagga aaccgagccg atgatccgtg ccgcgctcaa tctcggccaa
     4741 cacggccgtt tctgctggcg caccgctccc gacgccccct ggcgcacaca gagtgaattg
     4801 cgcgaacccg ttgtcgcgct ctccgcggcc gaacgcggga ccggccaaat cgagattgcc
     4861 tcgcccgtta cgcgccgcat gctgcgcctg acctttgacc ccgcccgcgc ggtgtccgtc
     4921 accctgcgtc tgcatcaggc cgaagcggaa agcgtccgtg tccagatcac ggaaaaaccg
     4981 gaaacgctcg cccccggtgc ggaacgggtc tgtcccctca cgctggagat cgcggatccg
     5041 cccccggacc tgccgggcag cctcgcaacc gcttttgtca cgccgcccgt tggccagccg
     5101 ccgcatatcg tctggcaggg ccagcccgcg gcgcggatcg tagtgccggt ccgcgccacg
     5161 ccggtcgagc gcagcgccgc gcgcgaactg acactgcacc tcgaagccat ctgcggtgcc
     5221 gcttttgaaa ccgtcaccga ggacgagccc aaaaccgggc cccgcctgct ggtcggcaat
     5281 accgatgcgg cacggcgtct gattcccggt ttcgatccgg cttccctcgc ctatgacggc
     5341 atcctgctca agaccgcggg cgatgacctg atcctcgccg gccacccgca acgcggcccg
     5401 atctatgccg tccacacctt tctggaagat tccgtcggcg tgcgctggtg gacctcggac
     5461 gaaaccttcc tcccgcaaca caccagcctg ccgctgccca cgctcgatct ccgttacgaa
     5521 cccaaactga aattccgcga aacctactat ctggattcgt tcaacgcccg cttcaaggta
     5581 cgcatgaagg gcaatttcag ctcgcgcaca cgctttatgc tcgacccgct cgagatgatc
     5641 cccgaggcct acggcggcaa ccaccggctg atccacttcg cagggcgcaa ctcggcctac
     5701 cactcgttct acgaactgct gccgcccgcc aagtatttca aggcccaccc ggagtggtac
     5761 agcgagatca acggcaagcg gacgcacacg cgcgcccagc tctgcctgac gaacgaggag
     5821 atgcgccgcg aactgacgaa gaacgcgctc gccctcctgc gcaccgaccc cggcgcggac
     5881 tttattcaga tcagccagaa tgaccacgct ggccgctgca cctgcgcgcg ctgtctcgcg
     5941 gtcgaaaagg aggagggcgg tgtcggcact gcctccgggc cgctgctccg gttcgtcaac
     6001 caagtggccg aagcgatcga aaaagagttc ccgaacgtct ggatcgacac ctttgcctac
     6061 cagtataccc gcaaagcccc gacaaaaacc cggccgcgcc ggaatgtgct ggtgcgcctc
     6121 tgcgacattg aatgctcgtt cctccatccc atcgagggta cgccgcagaa cgccaaattt
     6181 ttgaccgacc ttgtggagtg gagccgcatc gcgccgggga acctcttcct ctgggattac
     6241 gtcaccagct tcagctccta catcctcccc caccccaatc tgcgcgtctt ggctccgaat
     6301 gtccggacgt tcgtgaagca cggcgcgatc ggtctcttcg aacagggcga cgcgctctgc
     6361 cgcgcgggtg atttcgtgcg cctgcgccaa tgggtgctcg cgcacctcct ctggaacccc
     6421 gacctcgacg cggacgcgct gaccgacacc ttcctcgccg gatactacgg accgcgcacc
     6481 ggcgcggccc tgcgccgcta cctcgatttt ctccatgatc gtgcggcgcg cgccgatgtc
     6541 tacctcggtt gttatcagaa caacgtcacc cactggctcg acgccgaagg catcctcgaa
     6601 gcgctccgac tcatggatgc ggccctggcc gaagccaagg ccgaagagca ggccgatccg
     6661 gtcggacgga aaggtctggc ggataaaatc cgtcgcgaaa aactccccat ggatcacgtc
     6721 gtgatcctca accgtcccct gctgcaacgc catgccaagg ccaccggcaa acccttcccc
     6781 gtaaccgcgg acccgctggc cgcggttgaa gcctggatcg ccgcctgcga acgttttggc
     6841 gtcaaggcgc atcgcgaaac caccacggcc gacaccttca tcgactacaa gaagagcctg
     6901 ctggaacgcg cccgcatggc ttcacaaaag gtcagcccgc ccccctgttg cgcaaccctg
     6961 cctcccgcct cctggcacga tgtgcaggag ttcgagttcg atcagcggcc cgcaggccaa
     7021 gttgccttcg tggaggacaa ggcggcgtcc aacaagatgg ccgcgcaaat gcccggcaac
     7081 cacaagatct gggccgtgca gttcccgctg acgtccttca tacccctgtc cggaacggcg
     7141 tccggcacgc cgcgttgcac actgcacgtc tacgcgcgcg ccgatgcgac cgcaaccgag
     7201 ggtaacggac tcgcccttgg cgtctatgat ttcacggcgc gaaaaaccgt ggcgcaccag
     7261 tggattccgc tgtccgaact caccggcccc ggctacaaga tgtttaagtt cggtcctctg
     7321 ccgatcacgg agggctgtaa attctgggcc aatccgccgg aacggacggg cgaggttcag
     7381 cacgtatacg tcgaccgcgt cgtggtcgtg gtcgaataaa cgcgcataaa gttgacgcgg
     7441 ataaacactc cgcatgagga aaggagtcat catgaaaaaa atggtgttca tcgtaaccgc
     7501 aggactcagc gcctgcctgc ttcacgccgc ctcatgggtt cctacccagc cgtctccgga
     7561 cgaagcggcc atcgcctgta tcaacacgtc cgtcacaaac ggaaccgatg tggacatcac
     7621 ggcaaccctc gccagcaaca cggccgaggt ggtgatcggc acggccctca gtgaccgcca
     7681 caccttcctc cgcgccgtgg acctcagcgg acgcaccggc acgggcctcg gatccgccga
     7741 caactcggcc ttccatgcgg ctggcgtgga cgatgccgcg accggtgccg ccatcctgct
     7801 caaacggttg agcggcgatg tacacggtgc gggcgtcacc cacgccttcg gtctgctcgc
     7861 ctggtcggac ggcgccctcg gcaacacccc ggccaccctc cagatcgatc acctcacggg
     7921 aacggtgagc gccaacgtcc accccgacgg tacggctgcg gccctgtacg cgcgcaccgc
     7981 cctgcacctc aacctcatgc ccggcgcact gctcttcggc ggcagctacg gatgggacct
     8041 cggggaccag accctgacca atgcccaagc cgaactggtc ggcctgctcg ccaaagtcag
     8101 ggagggcgcc gcaaccgcgt cggaaagcaa cacgctgaaa acagccgctt ccggatatgc
     8161 catccttggc ggcgaagagg acgacttcct ccggttcggt ggtctggcca ccctcttcgg
     8221 tcaggtggac ctgggtgacg gcatggatgt ggtctcgctc tgcgacctgc cttccaacac
     8281 ggtgacgcgc ctgcccgcga tcgcgaacgc ggagctgctg ctgatcacca actccccgat
     8341 cttctcggcc tcccttgccg gcgctcccaa agaaaccgtg atcgcgggcg actccttctg
     8401 tacattcgtt gacgagctca ccacgctgga ttcgctctcg ggaaccggca cgctggcgtc
     8461 ggaagggacg atctgggtca ccaacctgct aagtgtcagc gggtttctcg ccgcaccgac
     8521 cggctcggcg ctcatcatcg aaggaaacct ggggctcgcc gacgggcttg cctgttccct
     8581 gcgcgcctgc ggcaccgcag acggatggac caacgaccgc atcaccgtga cgggaaccgt
     8641 gaccgtcgac ggcggcggca ccgtggatct cggctgcacg ccctccgacc cgattcccga
     8701 aaaggagcgc cgaatcctca tgaccgtcgc gggcggggtc atcaactccg ccaacctcgc
     8761 ctcgtggact ctgaccggca cgggatggga tgaagatccc agcctgatcc gccggatcgg
     8821 tacagacgga gttaccattt atgcgtccgt cttaaaaggc ggcacactcc tactgatgcg
     8881 ttaacgacct ttctggagga acaacccatg aaaaatcttt ccaagcctct cgcaatcttc
     8941 tcgatgaccg cctgtctggc cggcaccgtg catgcgcagc agattctcac taccgtgcag
     9001 aacatcaccg ccgctcccgc gccggccatc acttgggcca cccaacccga cggcgtgtat
     9061 tcgcttctcg cttcggacac cgagggcgga ccgttcgaca cggtcctgat cgcctccacg
     9121 cagaccgtcg aaaacagcat cacttacgtg gactggcgtc aggcaacgga aggcacgcgg
     9181 cgttactacc gggtggtgga aaacacaccg ggatacgcga cctacatgat cgtcgacctc
     9241 tccggggggc cggatgcgac ggtttacccc gtgtcttata ccaacgccgt gccggacctg
     9301 ctcacggaca gcgcgtacaa aaccaacaaa atggtcttcc gcctgattcc cggcggcagt
     9361 aacatcaccg gcttccccga ggatgtgccg cgcgggcagt atacccccta catcccctta
     9421 ggcaccatga ccctgacccg gccccattac atgggcgtgt tcgaggtcac tcaggaccag
     9481 tatgccctgg tgatgaacac caacccctcg ccctccaacc gcttgggagc gacccgtccg
     9541 gtgcaggatg tgacctataa cgacatccgc ggttcggccg cgcagggcgg cggcggctgg
     9601 ccgagcaaca gcgaggtcta cacgaactcg ttcatggggc gcctgcgccg gaaaaccgga
     9661 tacgaagagt tcgacctgcc cactgcgggc cagttcgaat tcgccacccg cgccggaacg
     9721 acggccctgt tttacaacgg tttcaacgac ccgacgaaca caaccgactt ctccggcatc
     9781 accaatatcg cccgctgctg tggcaaccag ttgtcgacat ggggcggata cacttacgcg
     9841 gcctcggtgg gcagttacct gcccaatgcc tgggggctct acgacatgac cggcaacctc
     9901 agcgaacact gtctggattg gggcggtagg aactcaggca ccgatcccgt cggcccgcag
     9961 agcggcactt cccgtaccgt gcgcggcggg gcgcacagca cgagcgccct ctccacactc
    10021 tacgccggag cgcgggactc catgaacccc ggcaaggtca cgcagagcgg cgtgttcgga
    10081 ttccgcaccg ccctgcgcct gcccgacacg ccgtccgagg agatgcgctc ttctgtctcg
    10141 gcaggcacca tcctctccgt aaccggaacc ccccgtcccg cttcccggat cacctggcaa
    10201 accgtgcccg gtaaaaccta tatcgtgctc tgcgcgacaa acgagacggg gccgttcgaa
    10261 acccagatcg gcgacacgct gaccgcatcc ggcaacacgc tcagcgtgac ggataccggc
    10321 tacacgcccg atggaccgca gcggcactac cgcgttgaag agttgcccgg cggctatgca
    10381 cggtaccgga tcgtggacat ctcaggcggc accaacgccg catcgtggcc ggtcacccac
    10441 accaacgtgc tgccggacat caactccgcc ctctaccgga ccacccgcat cgccttccgg
    10501 ctgatcccgc ccggaagctt caccatgggc gcgccgctct ccaccgtggg cgcgaccaca
    10561 aaaaccacgc cccacaaggt caccctcacc cggcccttct acatggccgt gttcgaaacc
    10621 acagaggcgc aacactcgct gatctatggt gacggctcgg gcacctcgca atatccggtc
    10681 gggaacgtga cctacgacat gctccgcggc agtaccagtg actggccgct gagcagtaat
    10741 gtcgcgccgg attccattat cggaatgctg cgcgccaaaa ccggcggcgc actggatttc
    10801 gatctcccca ccgaggcaca gtgggagtac gcctgcaggg ccggtaccac ctcggcgttc
    10861 aacaacggcg acgacttgaa gaccgaaacg gatttcggta cgctaaaagc aacgctcggt
    10921 ttgaataaca ggcatgcggg aggtgcctat caacccaact catggggtct gtacgacatg
    10981 cacggcaacc ttctggagtg ggtgctggac cgttggcagg aggacctcgg cacagcggac
    11041 gtggtggatc cgaacggccc ctccaccggc acaaagttct ctacccgggg aagtgcctat
    11101 atggcgaagg atagctttaa gtacgcctcc ttttcgcggg agaacggggg aatgaaccgc
    11161 tatggctcaa ccggcttggt ctcctaccgg ctcgcctgcc gccttgactg accgcaacgc
    11221 ataaacaaag gaaacaacat gaacgcacat atgaaaatcc tcaccgtcag cctcacgctc
    11281 gcccttttat ggggcttctc aggctgccgt ccgccggaga ccgcgcgaac gccgcgcgat
    11341 caggcgcttg acttcatccg caacgaaacc cagttccacc tcggctacct gccgaccgaa
    11401 cagtcacatc ccaaaacgcg cggcctctcg caggcgttgc aggccgacac cgccaaaggc
    11461 atccgcatgt tgttgagcgt ggacgaggac atcccgcccg tggcccgccg cgtggccgcc
    11521 tcgccggagt tcgccgaact gcgcaccgcg atcaagaccg cgctggacgc gaacaaaaaa
    11581 gtctacttca gcggttgcgg cgcgaccgga cggctctcaa tcctgcttga tgccgccaac
    11641 cgccgtttct ggcgcgagac ctacgaacgc cacccggacc tcaaagccgt gtgcggcgac
    11701 atggccgggc gcacccacgc ggtcatgacc ggaggcgatt acgcgctcgt gcgctcggtc
    11761 gagatgttcg aggactacat caccttcggc taccaccaaa tggagcaggc gggcgccgca
    11821 gagggcgatg tgatcgtggc gatctccgaa ggcggcgaaa cctcgtcggt catcggcacg
    11881 atcctgcgcg gtttggacgt caaggccaaa gtcttcttcc tgttcaacaa tccggccgac
    11941 ctgctggccg ataagctgga gcgctgccgc cgggtgatcc gcaacgacgc ggtcaccaag
    12001 ctcgtgctct gcacgggccc catgggcgtg gccggctcca cgcgcatgca ggccacgacc
    12061 atcgaaatgc ttgtcgcggg catggcctat gaggctggcc tgaaagaaca tctgcagacc
    12121 aagctcaact ccacccagat cgcctcgctc gggctcgatg cgcgtacgcc cgaaaagacg
    12181 ctcgaacagt ttgacacgct gctggggcag atccgcagcg agcggaacgt gaccgccctc
    12241 gcgcgactgg cggactatga agaaagtctc tacacgcgcg gcggacgcat cacctatttc
    12301 gcggacggtt atctgctcga tatcttcacc gataccaccg agcgtgcgcc gacctttaag
    12361 attccgcctt tccgacgttg cgacgacacc ctctcccccg cgccctgggc ctttgtcaaa
    12421 gaccccctgc gcaccacacc ggaggcgtgg ctgcgcgtgt tggagcatga gccgcgctgt
    12481 ctcgactggt cgcccgaaac ctacatccgg ctcggtgcct cacccgatat ccgcaagaac
    12541 ccgccgagaa tcgaccgcgc ctcgctccac acgttcctca tcgggaatga gccggacgcc
    12601 tcgcgtaccg agatccggcc caacgccgcc atggccgtgc tgatcggcga cgaagccgcc
    12661 gcgctggcac ccgccgcgaa cgcgccgtgg cgcaaggcct ttctcgatgc cgccaaaccc
    12721 tttgatgtcc gggccgcgct ggtcatcggg aaacaggccg tcgcgaatgg cgatgacacg
    12781 ctcatccatg tggacgtgga cattccggaa accccgctca acctcttctc gcacctggcg
    12841 gtcaagctga cgctcaacaa cacctcgacc gcctcgatgg gcaagatggg gcggctcacc
    12901 agtaactgga tggcgcacgt tgaagccacc aacaagaaac tgctcgaccg cagcacccgc
    12961 ctgattgtcg aactggccgg cgtggattac gagaccgcct gcatcgcgct cttcgaatcg
    13021 ctggaggaga tgaagacgtg ggacgaggcc cgcaaaaaaa ccgtctcgcc tgcggcgtat
    13081 acggtgaaac gcctgaccgc ccgtgccaaa cccggaaaat gagccgtacc tgaaaggtgt
    13141 ccgcgatgat tcgttccgcc ctgccgcacc tcctccaaac gctcctcttg gcggccctca
    13201 cagccgcccc atccttgtct ctatgcgcgg aggatgtggc ggtgtcgccg ccgctcgatt
    13261 ggaccctcgg cgcaggagac ttgaaaggcc cgctgacctt cttcggcgcg gaaaccatgc
    13321 ggaccacgaa tgtcgtgtgg caaggccgtg atgcgctgac cgccacatgg acgggccacc
    13381 cagagtacgg aaatgcattc accgtgaccg tgacttggca acgggctccg gacggcctgt
    13441 ggagcggtgt tctggcctat tccggatacc ggggatcccg gttcatcgag gagattcact
    13501 ttccccggct cagcaccgca tgcgacacgc acgccgcctt tctgttcggc ggcaaccaca
    13561 gtggccgcct gtacgccgtg ggaagcggac ctctcaagcc gggcagccgt ttccgccgcg
    13621 actattgcgg cggcatgcaa ttctccgccc tgctccgcac gaacgggaca tcgctttatc
    13681 tggaccaccg cgacccggcg gtcagcgtga aagagtgcga gatcgccatc cgcgccgacg
    13741 gtcgccgctt cacctatacc ggcatccaca tcccggggct tcccgagaaa ccggctgcag
    13801 actaccgtat cccctacccg agctgccaca ctctctttca gggcggctgg ttcgaggcgg
    13861 gccagcttta caagaagtgg ggcaccgccc agtcgtggca cgcctcgcgt cagggcgtca
    13921 atccgatgcg caagatcggc atctgggtct ggaaccgcgg ccttattaaa gacgtgctgc
    13981 cgccggtgga acggctgcaa aaagagttgg gcgcgattcc cgtggctctg gactggtact
    14041 ggtggcacaa caacccctac gacaccgact atcccgattt ctggccgccg cgcgaaggcg
    14101 tggagccgtt ccgcgctgct gtcgcccgcc taaagcggca aggcatctac tcgcaggtct
    14161 acgtcaatgg cgtctgttgg gatatggacg gcaaaacctg gcatcagggc ggtgcccaag
    14221 acgtggtggt gctgcggaac ggtcagccgc gcaataccgc cttcaacaaa tacaacctgc
    14281 accgtctagc ctacatgtgc ggcgaggccc cgcactttca ggataagatc gcgtcgctga
    14341 tgcgccatct gtgtgacagc ggtctggacg ggcaatatct cgacatgatc ggcaactcca
    14401 cgatgcgcta ccggtgctac aacccgctcc accgtcatcc gaaagggggc ggcacctacg
    14461 ggaaggacgg cttccgtgca ctgctcgaac gcatccgccg cgagaacccg aactatccgc
    14521 tgaccaccga aggctgcaac gaggcgtaca tggatctcat ggacgggtcg atcgtctgta
    14581 actccaccgg tctggaccgt ctcgacgcgc tccctctgtt catgtcggtc tatcacggcc
    14641 gctacgcctt tttcggcaac tacgcttatc ccgacggcac gcggccgtgg gatccgctgt
    14701 ggccgcctga ggaccgctgg aaagaggaga aaccctggca ccgcctctat cccgaccagt
    14761 tcttcgcgga actggcgcgg accgtctcgt ggggctgcca gcctatggtc tgcaacatca
    14821 aagagaccct cttcaccgat cccgatctgg tccccaacct gcgcttcatt ctcgacagcg
    14881 cgcgcttcta ccacgccaat ctggatgttc tgttcgacgg acagatgctc tcccctgccg
    14941 gattcacctg cgcgacggcg ggtgtagact atctgatccg cagtatcttc accaaggagc
    15001 atgagtgcaa accccaacat gccaaactac cggcagtcct gcattcatat tggcaggcgc
    15061 ccgacggcaa gaaggcgctg atcctcgcga actggacccg cgccagccaa ccttggtctt
    15121 tcaacgggct ttccggagtg atccccgcgc gttcctacgc ccggatcgcc ccatgaaaca
    15181 ccgcgtatgg acatcgagtt ccattactac atcacccacc tgatcgccgc gcgggcgggt
    15241 gtccggggtg aggcgttgcg gatcatccct catgcctgcc aactcacgga tgacaatgac
    15301 caggtctata cgatccgtaa cggccgacag ggaatttacc gcaaccggat ctcgcagacg
    15361 ttccagccgt tgaattcgca tcgggagcgc ctcgcggtct accctctctt ccatttcatc
    15421 cccggcgacc ctcatgcccc gggcagcgca cgcgtggacg gacagacaca cccgttcaac
    15481 acaacgccaa acagccccct cgccaaccgg attctggatg ccaccctcgc aacccacgat
    15541 ccgtatcaga tcggaatcgc atgccacgct tatgcggaca cgtgggcgca ccagaacttc
    15601 gtcgggttca aatgcgactt caaccggttc gatgatttcg tcgcccggct gatccccaac
    15661 atcggccacg ccgacgtcca aacccggccg gaccagcccc ggctcgtgtg gcgtgatgag
    15721 cgcctcatgg tccccgtggt gaacaacaag cagcggtttc tcgacgcggc cgcggccctg
    15781 ttcgccaaac tccgcgcctg ggccgcaccg caacaccccg attcggaaag cgagcaccgc
    15841 gcactgcgca ccgacctcga tacggcgatc ggcacagacc ccggcgagga gacggccgac
    15901 gccgcacgcc tcgcgggtta ccggcagttg gcggaacacc ctgcatacgg cgcgacaccg
    15961 attccggaat acgcaccgga cgcctggttc cgctccgcag tgcgggtgat ccggcaacgg
    16021 ggcggcaacc cggggcccgg tagacggatg cgaaaaggat atgtgttcag aaccggatac
    16081 gagttttcgg attggtaccg gttccaaaac gccgtcgtgt cctacgcgga acgtacccgc
    16141 gccctccttt gcgagattcc cgccttggcg gaggagtatc gccgcgcgta ctaatccggc
    16201 cacaaacagc atcgcacgtc gaacaccaaa atgcccctgc ttttcatgaa gtttcatgtg
    16261 tttcagggtt tcagggtttc agggtttcgg gtttcatcca aatcgctatc gctatcggta
    16321 tcgctatcgg gatcgattgc ttaacctaaa aagagcagtt gaaccttcaa ctctctttgg
    16381 tttcagggtt tcaaggtttc agggtttcat ccaaatcgct atcgctatcg gtatcgctat
    16441 cgggatcgat tatttaacca cgaacacacg ggatggcact gctcgggttt ttttggaacg
    16501 ccagagggaa ttcctgattt tcatgaagtt tcatgtgttt catggttcca ttccaatcgg
    16561 gatcgctatc gggatcgcta tcgggatcga tcgtttcaag gtttcagggt ttcagtagcg
    16621 cgtgatcccc ttgaaacggg attgccgccg ggagcggaaa tcgcctatgc ttgactcact
    16681 tgacccgcga tctgttccgg gtccgatccg caccgaaagg ggataagcga gaatgacgtt
    16741 tctgttccga cttctacgcg agattccgcc gcgcctgctc gggtccttcg tgatcaaagc
    16801 cgggcttggc aatctgcggg cgatccggcg tttcgagcaa cgcaaaaaac agggcgcggt
    16861 ctttccgcct tttctcttca tcagtgtgac ccaacgctgc gatctggcct gcaaagggtg
    16921 ttgggccacc ggggtggaaa agcccgttga catgtccgag gcgctgctgc accagattgt
    16981 caccgagggc cgcgaacagg gctgccgatt tttcggcatt ctggggggcg aaccgctgct
    17041 gctgccgtgg ctacccgatt ttttcaggcg gcaccgcaat ctctatttcc agctcttcac
    17101 caacgggcaa cggctggatg acgcgctggc ccgcaagttg cgcaaggcag gcaacgtctc
    17161 cccgctcatc agcatcgagg ggaatgccct ctcctatgcg gcacggcggg gggcgggcac
    17221 agcctacgaa accgcgctga cagccatcga tgcctgtcgg cggcaggggc tcgtcaccgg
    17281 cgtggccacc agtatctcga cctcgaattt caacgatgtc gtgaaccccg cgtttctgga
    17341 cgacatggtg gcgcgcggcg cccattacgt ctggtattac ctctaccgcc cctccggtcc
    17401 ggaacccggt ttcggagagg cgctgaacga tacccaagtg cgcgaagcgc gccgcttcct
    17461 actggagcag cggcggcgct gccgcacggc agtgatcatc gacgcgtatt gggatcacga
    17521 gggcaacgcg ctctgcccgg ccgcgaccgg catcagccac cacatcaacg cgcgcggcga
    17581 tgtggagccc tgcccgccga tccagtgcgc cgacagccgc gtgcggcctg acgcaccggt
    17641 cgcagcgacg atccgcgact ctcccctgct ggccgcgttc cgcagcgagg tgccgcgcct
    17701 gacgcgcggc tgcgtgctga tggatcatcc ggcggaactc gccgcgttgg cgcggcgtca
    17761 tcaggcatac gatagcagcg gtcgcggatg tttcttcgag gaactggcgg cacgaccgat
    17821 cctcggctgc cacaaccata gcggcgaacc cctgcccgaa acctcttggg cctaccgctt
    17881 cgccaagaaa cactggttct tcggcttcgg ggcgtacggc tgaaacagtg ccggcctcat
    17941 ttcgggatcg gtaccgacgt gagccactcc ggatgctcgc gcacgccttc cgcctcgatg
    18001 atcggccaag tcgcacgcgc cgcatccggg tacgggccga ttttgtcaaa cgcgatcggc
    18061 tcaaaaccca gtttcagggc gggcgaatcc ggtttgagcc ggaagtcgcc gcgcgccgcg
    18121 tcaacaaact gcggatcagc gaccacagaa tcccggtcgg cacccgccat ctgccatgcc
    18181 tcccactcgc tcgccggctc aacctcctcg agccgcagac cgctgatctc ggcccatccc
    18241 tgtttggatt ggaactcgaa gtggagcgtg aacgcttcca tacgcgcatc gaacccggcc
    18301 tctccgggtt tcggcagata gaatccggtt tcgcagggta gcgcctcgga ggcagtgctg
    18361 atatccgaca tgtcagaacg tttgaggaac ccccgcggac ccaaggcttt ccacaacccc
    18421 ttgttctcgc agaccagacg cacgagcacc tcgccgtccg cctcgtgatg acgcagcgca
    18481 aacgtcaggc ggtaatgctt cccgtacggc aacgtgaacg gcgcggaacg gacgcagggg
    18541 tatttaatgt acttgcgttc tccattaaag gcaccgaaca gacgcaacgc ctgagcatcc
    18601 ccacgcgtca ctcgttccgc ctgcagcccc ggcaggatct tctggtacca gaaccactcc
    18661 gccgcaatcg tctggttcgg atccgacgcc aacgcggccg cgtccaccgc acgcgcgaat
    18721 gcggcattgg ggatgcgctc cgtcaggtcc gcgattggcc tccggacggc ctgtttgccg
    18781 gtcttcaccg gctgggcacc gccattccac accacattcg aattgatgag gttgtgcgtg
    18841 agattcaccc cgttttcgcg gatatagcgg gagtccggct gctcggaata gaggaagata
    18901 ttgcggaaga tgcggttccc ccgcataatc gtgccatccg gcagaaacgg atcggcgggc
    18961 gacacatgca tcccgcgcat cttggcccac tccggattgg ccatcaggcg cgtataagcc
    19021 ttgagcattt tgggctggcg gtctctcagg aagaccgcgg tcggcgagtt gttccaagtt
    19081 tgcagggaga actggtgcgt cttgccctcc gggccggcat tgttggcgaa aatgttgttg
    19141 tagatgtggt tctcgcgggc attgtgcaga tgcatcgcgc cgacctgaca gcgctccacg
    19201 aggttcccgt agacatcatt gccgcagccg ctctcgtcca gatagatacc ccaggcgaaa
    19261 acgaaaaact tgccgtggtt gttgaacccg atactgtcgg agcagtggtt gtagcggatc
    19321 gtgtgccagc ctccgcacca gccgccggtg taagtgcagc ccgtgtcctc catctccaga
    19381 ttgcagtgac ggatacggtt gtattccagt gtgtggagca ccccgccgtg gaaaatcccg
    19441 ctccgcggca ggtcgtgcat caggttgtgc gccacacgga ccccggcacc gccgatcatc
    19501 acgccgaggc cgtggcggtt gaactgaccg acatgatgga tataacagtt ttcgacctca
    19561 tgaaaacagc gcgtcatcga atcgacatct cccgcataca cggagacgcc gtgcccgccg
    19621 atgtgccaga tgtcgcaccc gcgtatccgg ccttcccgac cgccccggat cccgatgccc
    19681 gaaccgctga aaaacccgag atcatggatg cggcacgcct caaccgtcat gtgatcgcaa
    19741 tcctgaaacc ggagggcgtg cgtctcggcg caggtcagtt ccagattcgc gatccggacg
    19801 tggtgcgtct tgttgaagga gaggatgtcg ttgaccgtgg gaaccgtgat ctgatcccgg
    19861 gtgagatccg cccctgcggg cgggatcaga tacagccgct gtcccgcaac atcctgaaac
    19921 cactcgccgg gcgcgtccaa ctcttcgcgc agccccatga cgcaataccg gtcctcgggc
    19981 cgtgcggcgt acggcatttt ctttgcgagc gtgaacgtgc gcgaacccgg atcaaaggcc
    20041 gcgatcttct caatccggtt ccaccagttg tagcgcggga aaatgcacat ttccccctcc
    20101 tccggacggg accaggcgtg cacgtcccgc tcgcgcagca ccaccgtgtc cgtgcgttca
    20161 ccctcgatat ccttgtacat cgggggccgg accccgtcaa cgtaagccca cccgccggag
    20221 tagggcaaag ccggatccgc attggggtaa cgcgcccaga tcagacgctg accgttgaga
    20281 tatacctgac ggaacggcgt ggtaatgccg agcggtttaa gatccgcctc gaacaccccg
    20341 tcccggccgt tgaaggagca ggccttccag ccggtgacca ccttgccgcc aaccagaacc
    20401 ggccgcgcct ccggcggtgt tgacggtgtg cgccacgcca agagctgtaa cggcgcctcc
    20461 cgggtacccg cgtcctccgc cgtgaacgcg accgtccggg tcacacggtg cgtgccgggg
    20521 tgcaacagaa ggctcaccgg ctcgcccggc tgacgcgcgc gcgcctcccg cgccagcaga
    20581 cgggccttct ccagcgtcag caccggctcg cggacagaac ccggtgcctc gtccgatccc
    20641 ttgggcgaaa catggatctc cgcgcccagc gccattccgc acacgatgaa gaccccttca
    20701 acgcaacaca acaaacgccc tagccgtttc gcaaaacaca tacagaacac tcccccttat
    20761 aacgcaggat gataaaaacc aaaggacaac acagcgacaa atagtacccc aaccaccatc
    20821 aaagcgcgag aacagatcga tatgagaaaa ccgcctgccg agatttgggg tgtatcaagc
    20881 catgcggctc acggggacgc tcgccctccc aaaaaatctc tgtgtctccg cgtctctgcg
    20941 tcatcaagaa gcgcaatgct atttgtggcc ggtttagtaa ggacacaggc agtgtggcac
    21001 accgtgtcgt ccccgtcaac ctatacctcc cagcccttgc ggtagctgcg cttcaggaat
    21061 gcgttcgcgc tctccttgtt ggtgaagcgg ccaaccttgg cgtcccactt gagttcatcg
    21121 ccgctgtctt ccatgatcgc cgtcacgcc
//
LOCUS       DAOSLE010000031        21142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2752_length_21142_cov_7.568739, whole genome shotgun sequence.
ACCESSION   DAOSLE010000031 DAOSLE010000000
VERSION     DAOSLE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2752_length_21142_cov_7.568739"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   33..3172
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=33..60
                     /rpt_unit_seq="cttctccccacacgcgtggggatgatcc"
     gene            3430..3639
                     /locus_tag="PK576_03715"
     CDS             3430..3639
                     /locus_tag="PK576_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60729.1"
                     /translation="MIRACRIVAGPGRWRASTPQEKGSYEGDAVTFVADTGERVSFRV
                     TAGKSAPRITLKTMWIEKQKDRPAD"
     gene            3841..6255
                     /gene="pheT"
                     /locus_tag="PK576_03720"
     CDS             3841..6255
                     /gene="pheT"
                     /locus_tag="PK576_03720"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE60730.1"
                     /translation="MKVPVSWLNEFVDVSDLSVRELADKLTFSGVEVEGVEEVGVILD
                     DAFVVGEVLTCAAHANSDHLHVCTVADGEQEWQVVCGAPNVRAGLKVAFARIGAVIPE
                     GGFTIKKAKLRGVESFGMLCSARELKLSDDHAGILELDAALKPGTPLRDVLPQPETVL
                     DLEITWNRPDCLSIIGIAREFAALLRRPLRLPPVDFAETEEPVESFAKVRVEDPVKCP
                     RYTARVMTGVRDGASPAWMQHRLELCGVRAISLTVDVTNYVMLECGQPLHAFDYRTLA
                     DHTVVVRCATPGETITTLDGVKRTLDPSMLVIADAKAPSAVAGVMGGAGSEIVTDSEH
                     VLLESALFDPAATKFTATRLGIGTESSRRFERGVDPDLADWASRRAVALLAEHGQARV
                     ARGVIDVDNRGWKAGEVTLRFARAREVIGVALPDDEMVGLLTSLGLEEIARDAQGATF
                     RIPSWRLDLTLEADLIEEVARLHGLDAIPDRLPDATAVSTLDEGAFRARSRCRQILLG
                     MGFSEAMHYSFLSAQELDAFDTRHAASRVVLPNPVSADYGVLRDTLLPQLAGSLGRNA
                     SRQVECAALFEMGRVFFKEANGVPGEEERVALGLMGPFGRSSVERRRPVGNEEALLWL
                     KGAVENLAAALHVGDLTFVRGEHPAMETGWAVGIELNGEQVGCMGLLSAALRHAWRMT
                     APMPLAELRLAPLLAGAGAAAAIQPVPQYPAVRRDIAFVADAAVTHREVVETIRRAGP
                     KELTDVGLFDIFQSKEIGKGKRSLAYALEFRSPDRTLTDQEVNAAFAEIARALKQNLH
                     VEVREG"
     gene            6316..6493
                     /gene="ssrS"
                     /locus_tag="PK576_03725"
     ncRNA           6316..6493
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PK576_03725"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6509..7354
                     /locus_tag="PK576_03730"
     CDS             6509..7354
                     /locus_tag="PK576_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60731.1"
                     /translation="MRRVLTGVAAVWLGCAVVSGQSWDATDQAEIDLGVTTGVPQASG
                     FVFIEGRYLAPPYTVTRKGNGLFINRTLVERPVPWPPAVTPQVPGTEKRVDGDGAAQP
                     QAEPQNEAPQIGERKKVLSVDDLFDDEDSDEETVPLAEDEVKRPVAEATAAAGNGLSA
                     EEIARRKAETLAVLERLRKGYEVALSQGEVFFFSQRHNRVNGTYGTARLLMGVLPAAL
                     RYAKSPQDLMSRLYQGGITFIDLTMCAALYGHRNTFPLLEARLARIEEAEREEERKRN
                     AVRRR"
     gene            7370..7735
                     /locus_tag="PK576_03735"
     CDS             7370..7735
                     /locus_tag="PK576_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trp family transcriptional regulator"
                     /protein_id="HOE60732.1"
                     /translation="MARVKQKRDGKSFRAVARVLAEINSTEEMAAFLDELLTPAEVYD
                     ISLRWKLLGLLAQGMSQRRIAEELRISLCKITRGSRILKQKGAVSARLLSDGKTDGTA
                     YGKTGGLKRNIRKGVGGCK"
     gene            7726..8910
                     /locus_tag="PK576_03740"
     CDS             7726..8910
                     /locus_tag="PK576_03740"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE60733.1"
                     /translation="MQVIAKAVQEQMGSASWIRKMFEKGAELKKAFGDEQVCDFSLGN
                     PDVPPPAKARDALCALADQAVRPYGLGYCPNAGLPAFREALADFLARQQDVPLRAEQV
                     VVTCGAAGALTSFFRAVLEPGDEVICPAPYFVEYGAYGGHFGGVLRPVPALPPDFQPD
                     VAAIEAAVTERTRVLLINSPNNPTGCVYTRETLERLGALVDRVNAARERPLFLVSDEP
                     YRFFAYDGVTVSPVLPFSPFSLVVGSFSKSLSLAGERIGYLAANPAMPQVKTLMDAVT
                     MTSRTLGFVNAPVLGQRLAMALLEEGVNLDVYDRRRQAMAAVLTQAGIRFAMPRGAFY
                     FFPEAPGGDDLAFVQRLLERNVLVVPGRGFGFPGYFRLSFCVEEAVIARSAEGFQAAV
                     AG"
     gene            8942..10309
                     /locus_tag="PK576_03745"
     CDS             8942..10309
                     /locus_tag="PK576_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60734.1"
                     /translation="MRTKFGIMRVTVAVLWACGVRAAEYTGPAEWRMVNTNGVRAWQA
                     AQQAAASGGTVAVWPGVAADPVRREVRLLAEAVGHTEGVTTEFILIGPMSDRAYEAVA
                     VTVAAPSDIVRAVEHLGLKRGGGVGSRPFRFWPVGERVTATVRRLDGTDAAVLPLQTL
                     IRDEGEGEPLVGEGGLVFTGGRWVGPSCLTDTNMPCSVISLYNAGETVFDVPFRVGQS
                     EVYGRLKLARSYPCGALIEVVLRPLAADGRPQVLSLRVTAEPGEGGVSLVTRGADGTV
                     LHRSALAEGLVWLRKQADTGRELFVTLEMDETLTLQQATEVARIFDMLDGKGLKLDGK
                     GEDGVYAKAFLPLERWREREGRTPQPFELHVKRNAEGGWTRSLVFIEEDWQVEGLDPR
                     LTPRTYPFEEWEALPGLTARAGGAENKVRVLFVFAPREASLRTFMPGVRALSGRLPIV
                     YVFGE"
     gene            complement(10319..13453)
                     /locus_tag="PK576_03750"
     CDS             complement(10319..13453)
                     /locus_tag="PK576_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60735.1"
                     /translation="MLILLRMILLFLGPVFLFVALLPTLLSTDAGRRFVLDRLNAAGD
                     IRLTVEKWSWGWFRAPVLEQVSLIDPERGMTVTAERLTSDRGLWRLLPLGTWNAGNLT
                     LEKPEVTLDLMPRPRADAAKPREPSRSRDGGFFLPILDIAAHAVIREGSLRVNGQSET
                     PFEAQQIEGQITLTSFRKPIEINGQMRIGGGTLGIGGRVQSLRALTAARGAPPAEAEA
                     LTVKLLGVDLTAVRPLLREWAGQEWVSGGKAEGAFTVTMTNLDHLTLKGGVLVTRLTV
                     AAAGHRPSPPGDLALMTDLTYERPSLTITRFDLSAPWVRAEASGALQTAGQGKTVPIG
                     TLRAKIAVDLAAVARDFGPALNLAKGLRVRQGVLQGTVAVQGDETALGLDAALAIADL
                     NLTVDGQPLILKPEPSLHLKASFPYGHGPTVETLRLKAPFAELYGSGRFDSAVVKGQV
                     NLTRFSRDFKRILHSVPPMVGSIYLDLATRREKGHAAVTGFLKVSDTSVEVRPGQRLT
                     VPLGTLKLNSRVPMKGDQPERELQDTTFDVILDKTKLAGGWKRWRPAQSGQPTVLRGF
                     TLAADTDLHSLRKTLGGLLPPPTLRRLASWQGALVANATAEAASGVVKARMNAALRQG
                     AARTAAGIWRVPDLRMEGTLSQSVQQPGLRCETTATGSAAFERDGETVYAEPAARLVT
                     DFTLAADGQSVRIDTFDIRSSLIEARARGSLSDLAVRRLLQAQGDMTVDFAAVTRLLD
                     ARGIDEFTLTGKAARPFRFSAPTAGGLTTLLAEGEFSGAAFLASAKGLGLDAGPSDVN
                     LRLAKGVLDTRYAPTLNGGRMVFVPQLRYTPDGVSLTVPEKSRLLENVKITQQMVDNL
                     LVNLNPMFHGSKIRDGTVTLDVRHFTYRPGTDLKTGLSADLSLRFDRLSLSLGPALRE
                     VLTFLKVKEQTYTVEHLPVHLVIRDGKTHVDPIRMVVGNQPIVFSGWVAFDGSIRYLL
                     QVPITERLTGGAGKMLKGTTISIPITGTVTQPRVDLNVLHNALGGLIKQTVGEENLQK
                     VQSFLEQLREEFRK"
     gene            complement(13597..14508)
                     /gene="epmA"
                     /locus_tag="PK576_03755"
     CDS             complement(13597..14508)
                     /gene="epmA"
                     /locus_tag="PK576_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOE60736.1"
                     /translation="MNPERLALRSRILHAVRAFFHAHDFVEAETPVRIPAPALETHID
                     VPPAGRAWLRASPELHLKRLLAAGSPRLYQIGPCFREGECGRRHNPEFTLLEWYRAHA
                     DYIAILRDTEALLTQVFQSVFGRTTLVYQGVKIDLEPPWTCLSVREAYQRFAGWDPVR
                     DWDPERFDLDMVHAVEPALPRDRPTVLTDYPAEAASLARLKPDDPQVAERWELYIGGL
                     ELANAYSELCDAETQRARFLAAAEARRQLGKVIYPLDEPFLDALRSGMPPCGGIALGI
                     DRLIMLACDASDITEVRPFCQRPGELW"
     gene            complement(14519..14770)
                     /locus_tag="PK576_03760"
     CDS             complement(14519..14770)
                     /locus_tag="PK576_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE60737.1"
                     /translation="MPVYVYEAVNPDAGCPHCVGSFDVTQSIHDARLTACPACGAAIR
                     RVITAPAIGRSKSKLDDRAKSAGFTKLKRLGKGEYEKIY"
     gene            15059..15940
                     /locus_tag="PK576_03765"
     CDS             15059..15940
                     /locus_tag="PK576_03765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE60738.1"
                     /translation="MKTVKVNGQRISEEAIQFELERLVRFHAEHGMAEDQVRAQLPAL
                     VDKAIDQAIGAKLLLDEASRLDIPVREEDVEAQIARIIEQVGGEDAFRKALTGQNTTE
                     QVFRDQIRRGCRVEKLVEQAVAGVAEPTEEDIRRHFDEHKEEYAPGERVLAQHILISP
                     DGDTQTSKDEARAKIEAIRERIQAGKSFSDEAAAHSMCPSGKDGGSLGWFSRGMMVPE
                     FDSAAFSMNVGEVSDVIETQFGYHIIFKTDHEAAGEADFDQVREKIRDFLRHARRGEA
                     MAAYVNELKSKAKIEVE"
     gene            15978..17006
                     /locus_tag="PK576_03770"
     CDS             15978..17006
                     /locus_tag="PK576_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOE60739.1"
                     /translation="MKNMRRLWVTVLCVAAMTYAEPVKLIFDTDMGNDTDDLMALCMI
                     HTLQSRGAVDLLAVTITKDHPQAAAFVDAVNTFYGRPDIPIGVVKGGVTPEPGKFNLL
                     AAAKNEDGSLRYPHDLTSGEQAPEATGLIRKILAAQPDQSVSLVQVGFFTNFQRLLDS
                     PADAHSPFTGKELIAKKVKVLSLMAGSFKPINWKTRHLEYNVIKDIPAAQKLAKEWPT
                     PMIWSGFEIGIAAAYPYESIERDYAYMKHHPLPEAYALYSAKGHNRPTWDPTSLLVAV
                     YPDRGYFTFSPVGTVTVENDGGTLFVPGKGRDRYLSMNAEQAERVREAIVQLCVAPPT
                     HAAALKNR"
     gene            complement(17212..18864)
                     /gene="sulP"
                     /locus_tag="PK576_03775"
     CDS             complement(17212..18864)
                     /gene="sulP"
                     /locus_tag="PK576_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013487924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOE60740.1"
                     /translation="MTWLRTYSRQDLRKDCIAGLVVAVMLVPQAMAYAMLAGLPPVVG
                     LYASTVPLLAYACLGTSRQLAVGPVAIVSLLVADACAGRPSGTAVDATTITGRAAMLA
                     LLVGAIQIALGLCRAGFLLNFVSHAVVSGFTSAGAMMIGLSQFGHLLGVKLSGGNSVL
                     ALALDVVRNLSDTHLPTLAIGLGAVAIQLLARFKRWRFPVTLPIIAAAILLVWAFNLE
                     EYGVRTVGEVSSGLPVWRLPDISWNGIRNLLPAAWMIVFIGTMESIAVVKWIATREKY
                     RIDANREMLGLGLANVAGGLFSGYPVAGGFSRTAVNYQAGARTPLASVFTAALVLLTL
                     LFLTPLFTHLPNAVLAAIILAAVIGLIDFKEARHLFKIKQADGWCFMVTFLVTLGFGM
                     DKGLLVGIAFSLLLFVLRSARPGMTILGQVGEQEFRDIQRYPDAVTLPWLLILRFEAS
                     LYFANAGFVEDRIREMLVQRPEVRWILLDMSGVNDIDGVAVSMLTELMRDCAAADVQF
                     ACAGMKAGVRDVVRRAGWDDDLPGSRMFYPNLQQALAALPSH"
     gene            complement(18876..20252)
                     /locus_tag="PK576_03780"
     CDS             complement(18876..20252)
                     /locus_tag="PK576_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE60741.1"
                     /translation="MLFQQFKVDGLGCFSYLLGCPASGTACVVDPERHVEQYLRAAQQ
                     HGVRITHIFDTHLHADHITGSNELAARTGAKIYVHPGVEAGYPHEDLHDGDRFRFGAA
                     ELEVIETFGHTPNSVSFAVTDHSRSEDVFALLTGDLLFIGDIGRPDLAGADLLEEQVK
                     NLYDSLYVKLARFPDWVEVYPAHGEGSLCGRGMSAKPVSTLGFERRNNPLLNNMSFEQ
                     FHKIMTTGFQVRPDNFAVMVDKNRRGPASLAAAAPFNLLNVKQVEEALARGAKIVDTR
                     VPSAFGAAHIRGSVNIGLTPLSVNWLGMVIPADTELIVVADTPARAEEAAYLYRRAGY
                     DRLIGYLGDGVSGWAAQAKPLDHLPQLTPESLKTVFEKYPDHLVVDVRSDLEWESGHI
                     EQAVHIPIGKIIAGDFDLDKTRHITVVCGSGYRGNIAGSILKSMGYPKVFSLIGGMTA
                     WKASQKNR"
     gene            complement(20516..>21142)
                     /locus_tag="PK576_03785"
     CDS             complement(20516..>21142)
                     /locus_tag="PK576_03785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE60742.1"
                     /translation="LGDKFPELSVKTTQGPMNIPGDLKGSWFVLFSHPADFTPVCTTE
                     FVAFQKRYDQFKALGCKLVGMSIDQVFSHIKWIEWIKSELKVDIDFPVIAANDAIALK
                     LGMLHPGKGTNTVRAVFVVDPEGVIRLILYYPQEIGRNIDEVVRAVKALQVSDSKKVA
                     VPAGWPNNELIKDKVIVPPPSDIADIEKRRKEYESFDWWFCYKNLDSF"
BASE COUNT         3991 a         6134 c         6911 g         4106 t
ORIGIN      
        1 ttgccgacgc tctgcccgcg cccggttacg tccttctccc cacacgcgtg gggatgatcc
       61 gttgtacgat tgtgctgatc tccatgcctg gctcttctcc ccacacgcgt ggggatgatc
      121 cgacgtgaca gcatgtcgtc aacagcggcc tcggcttctc cccacacgcg tggggatgat
      181 ccgattctgg tacatcgcct cgacactgcg gaaaacttct ccccacacgc gtggggatga
      241 tccgtttgcg ttctgcattg tccgcccaac gtgcctcttc tccccacacg cgtggggatg
      301 atccgtcgag gtgggcggtg gggtctggtg gggcatgctt ctccccacac gcgtggggat
      361 gatccgacca aactggtcga ggatcagaag gagcaactct tctccccaca cgcgtgggga
      421 tgatccgcac ggactgccgc gcctgcgcga gccagacccc ttctccccac acgcgtgggg
      481 atgatccttt ttataggggc gtgtatgcgg tgaacacgga cttctcccca cacgcgtggg
      541 gatgatcctg cgcatggcta gcgtttggag gaatggccag ccttctcccc acacgcgtgg
      601 ggatgatccg cggaatttct cgcggactca atagcagatt tccttctccc cacacgcgtg
      661 gggatgatcc cgtatgattc ggattaggat gcacattgca aaacttctcc ccacacgcgt
      721 ggggatgatc ctggctgtaa acgatgtgaa atcggctatc gatgcttctc cccacacgcg
      781 tggggatgat ccgggatttt ttcgttatat gattcgtatt cctcgcttct ccccacacgc
      841 gtggggatga tcctgccgcg caccccacgc gagagccatt tttgcgcttc tccccacacg
      901 cgtggggatg atcctgcgca ctggaaaaat cggccgatat ctcgcaactt ctccccacac
      961 gcgtggggat gatccgcgat ttatctgctc ccgcgccgac cctgaccact tctccccaca
     1021 cgcgtgggga tgatccgtat gggagagatt gcgatatcgg gattcgtgtc ttctccccac
     1081 acgcgtgggg atgatccgct ccgaccgttc tcatacacaa ccgaatgaat cttctcccca
     1141 cacgcgtggg gatgatccgc gcataaaaac cctagcaaaa aaacgcatac acttctcccc
     1201 acacgcgtgg ggatgatcca acgggcaact gcgatatatt gatcaggagc aacttctccc
     1261 cacacgcgtg gggatgatcc ggttagatga cggttttggg cggggcgcac acgcttctcc
     1321 ccacacgcgt ggggatgatc cgacggccag cgtaccgatc agcgggagga acagcttctc
     1381 cccacacgcg tggggatgat ccgcaatttc gtggaactga ttccgcgcgg cactccttct
     1441 ccccacacgc gtggggatga tccgcgcggc gcacgatctc gacgaggttt gcatttcttc
     1501 tccccacacg cgtggggatg atccgagccc cttttccttt gtcgcatatg cggttttctt
     1561 ctccccacac gcgtggggat gatccttttt gatttctaca gctgaaattc tccaaaaact
     1621 tctccccaca cgtgtgggga tgatcctttc tagggaattt cgtggaattg acccctcgcc
     1681 ttctccccac acgcgtgggg atgatccgac cgaggccgcc gccggatcgc ccgagtggga
     1741 cttctcccca cacgcgtggg gatgatccgg gcgcagagcg ccgtcgtgag tgtgtgtcgc
     1801 tcttctcccc acacgcgtgg ggatgatccg gcgagcatga gtctgccgcg cgggtctgcg
     1861 agcttctccc cacacgcgtg gggatgatcc gggcaccatg atgctatagt agtggcgttt
     1921 ccgcttctcc ccacacgcgt ggggatgatc cgtacaatgg ccgtccgtgg tcgcagttga
     1981 gcgacttctc cccacacgcg tggggatgat ccgtgattag tcctgattct catattccca
     2041 atctacttct ccccacacgc gtggggatga tccggatcaa cggtttattg attcactcta
     2101 cctcatcttc tccccacacg cgtggggatg atccgtgcct ttgattcgcc cttgaatctt
     2161 gaattagctt ctccccacac gcgtggggat gatccgaata ttagagttct tcctctagta
     2221 ctctctctct tctccccaca cgcgtgggga tgatccggtt ttgattcggc cttgaatctt
     2281 gaattagagc ttctccccac acgcgtgggg atgatccgac gccccggaaa ttttccgctg
     2341 aaaaatacgc cttctcccca cacgcgtggg gatgatccgg ggcgcgccga catcttctta
     2401 gttcccagtt gcttctcccc acacgcgtgg ggatgatcct catttagtgt ctttttcatt
     2461 gtctcctctg ttcttctccc cacacgcgtg gggatgatcc gttttctggg aacccgatgg
     2521 acggcttgcc aaccttctcc ccacacgcgt ggggatgatc catctcaatt taacccaata
     2581 aaatcggggt aaaacttctc cccacacgcg tggggatgat ccgtgtcacg ctccgagaat
     2641 gacattttga cgaaacttct ccccacacgc gtggggatga tccgtgcatt tttgacctca
     2701 aaaatcaaca attcaacttc tccccacacg cgtggggatg atccgcccca gcggaaaatc
     2761 tcgccgagcg tcagttcctt ctccccacac gcgtggggat gatccgcgcg ggcggtctgc
     2821 ggccaaatct gcggctagct tctccccaca cgcgtgggga tgatcctact gatactgtgc
     2881 cgtactggag ttgggatgac ttctccccac acgcgtgggg atgatccggc gcgaccggat
     2941 tgaatttcga ctccaccggt cttctcccca cacgcgtggg gatgatccga tttcataaag
     3001 agctaacaac tccaagtgag acttctcccc acacgcgtgg gggtgatccg cgatctgcgg
     3061 cgccgactac gccgaatgtg cacttctccc cacacgcgtg ggggtgatcc gccgccgccg
     3121 tcaacgcctg cgccaacggc aaccttctcc ccacacgcgt gggggtgatc cgattgagac
     3181 gtgggtcagc agcaccaagg ccggaacggt ggagaacaag cgcggcggac ggctggaaca
     3241 gcggcagggc ggataaatgg gagaccggga ggaacccgcc cctcccgatc aactcgcccg
     3301 gtcatcgccg acggaggcca ccgggcggcc acgcaaagac aacgtagcaa aaggacgaaa
     3361 ggagcgcaac atgaaggtga cagtcacagg aatcgaagac gcccatatct tctccccaca
     3421 cgcgtgggga tgatccgggc gtgccggatc gtggccggcc ccggccgctg gcgcgccagt
     3481 acgccgcagg aaaaaggatc gtatgagggc gacgccgtga ccttcgtggc cgacacgggc
     3541 gagcgcgtca gcttccgcgt caccgctggc aagagcgccc cccgcatcac gctcaaaacc
     3601 atgtggatcg aaaaacaaaa agaccgcccc gctgactaac gtccggaacg ctcccgtttc
     3661 cacacgggcc tcagctgctg cggtcaggag agagtatagg cgatgctgga catccaagtc
     3721 aacctcgacg gatcaggcgt aatcggcctt gtgagacagt gtgagattct ctggctggtc
     3781 tctgttgcgg attatgttat tcttttttcc tcattttgag cgaaagaggg gtttgcgggt
     3841 atgaaagtgc cggtcagttg gttgaatgaa tttgtggacg tgtcggattt gtcggtcagg
     3901 gagttggccg ataagctgac gttctcgggc gtggaggtgg agggcgtcga ggaggtcggc
     3961 gtgatacttg atgacgcctt tgtggtgggc gaggtgctga cttgcgcggc gcacgcgaat
     4021 tcggatcatc tgcatgtctg cacggttgcg gacggtgaac aggaatggca ggtggtgtgc
     4081 ggggcgccga acgtgcgtgc cggtctgaaa gtggccttcg cgcgcatcgg cgcggtgatt
     4141 cccgaaggcg ggttcacgat caagaaggcc aagctgcgcg gtgtggagtc gttcggcatg
     4201 ctctgctcgg cgcgcgaact gaagctctcg gatgatcacg ctggcatctt ggagttggac
     4261 gcggcgctga aaccgggaac cccgctccgg gatgtcctgc cgcaacccga gacggtgctg
     4321 gatctggaga tcacctggaa ccgtcccgac tgtctctcga ttatcggtat cgcgcgtgag
     4381 ttcgctgcgc tgttgcggcg gccgttgcgt ctacccccgg tggattttgc cgagacagaa
     4441 gagccggtgg agagtttcgc gaaggtgcgt gtggaggatc cggtcaagtg tccgcgctac
     4501 acggcgcgtg tgatgactgg cgtgcgtgac ggggcctcgc cagcctggat gcagcatcgg
     4561 ctggagttgt gcggcgtgcg ggcgatcagc ctgacggtgg atgtgaccaa ctacgtgatg
     4621 ctggagtgcg gtcagccgct gcatgcgttc gactaccgta cgctggcgga tcacacggtg
     4681 gtggtgcggt gcgccacgcc gggcgagacg atcacgacgc tggacggtgt gaagcgcacg
     4741 cttgatccca gcatgctggt gattgcggac gccaaggcgc cgagcgcggt ggcgggtgtc
     4801 atgggcggcg cgggcagcga gatcgtaacc gactccgagc atgtgttgct cgaaagcgcg
     4861 ttgtttgatc cggcggcaac aaaattcacc gccacacggt tggggatcgg cacggagtcg
     4921 tcgcgccgct ttgagcgcgg cgtggatccg gatcttgcgg attgggccag ccgcagggcg
     4981 gtggccctgt tggccgaaca cggacaggcg cgtgtggccc gaggcgtgat cgacgtggac
     5041 aaccgcggct ggaaggcggg cgaggttacg ttgcgcttcg cgcgggcgcg tgaagtgatc
     5101 ggcgtggcgc tgccggacga cgagatggtg ggtctgctga cgtccttggg gctcgaagaa
     5161 atcgcgcgtg atgcgcaggg cgcgactttc cgtattccgt cgtggcggct ggacttgacg
     5221 ctggaggcgg atctgatcga agaggtcgcg cgcttgcacg ggctggacgc gattccggat
     5281 cgcctgccgg acgcgacggc tgtctccacg ctggatgagg gggcgttccg tgcacggtcg
     5341 cgttgccgtc agatcctgtt gggcatgggg tttagcgagg cgatgcacta cagttttctt
     5401 tcggcacagg agttggacgc gtttgacacg cgccacgcgg cgtcgcgggt ggtgctgccg
     5461 aacccggtga gcgccgatta cggtgtgttg cgcgatacgt tgctgccgca gctcgcgggc
     5521 tcgctcgggc gcaacgcttc gcgtcaagtc gagtgcgcgg cgctgtttga gatggggcgt
     5581 gtctttttca aagaggcaaa cggcgtaccc ggcgaggaag agcgcgtggc gttggggctg
     5641 atgggaccgt tcgggcggtc gtcggtggaa cgacggcgtc cggtcggtaa cgaggaggcg
     5701 ctgttgtggc tgaaaggggc ggtggagaat ctcgcggcgg ccctgcatgt tggcgacctg
     5761 acctttgtgc gcggcgagca tcccgcgatg gaaacggggt gggcggttgg aattgaactc
     5821 aacggcgaac aagtcggatg catggggttg ctgtcggcgg cgttgcggca cgcgtggcgc
     5881 atgaccgcgc cgatgccgct ggctgagctg cggttggcgc cgttgctggc cggcgctggt
     5941 gcggcggcag cgattcagcc ggtgccgcag tatccggcgg tgcgacgcga catcgcgttt
     6001 gtggcggacg ctgcggtgac gcaccgcgag gtggtcgaga ccatacgccg tgcgggcccc
     6061 aaggaattga ccgatgtcgg gctttttgat atctttcaat caaaagaaat cggtaaaggt
     6121 aagcgcagtt tagcctatgc gctggagttc cgttcgccgg accggaccct gacggatcag
     6181 gaggtgaacg cggcgttcgc cgaaattgcg cgggcgttga aacagaacct gcatgtggaa
     6241 gtgcgggagg gctgaccgac tatggatctt tgaaagctcc gggtcccgtt tccggacccg
     6301 gacaaaaaac cgaagtggac cctgctttgt gcgcggataa gcagcatttc tctgaccttc
     6361 aatttgttac gggagtttgc gtccggcgcg ggacggatgc ctgccttgcg cgggaaccgg
     6421 aaaacgtcgg cgaaagcgcc cacctcattc gtgaggttcc agaggtgctg tcatacggca
     6481 aagcggggtt ttttcggatg ggggacgtgt gagaagggtt ttaacagggg tcgcggcggt
     6541 gtggctcgga tgcgcggtgg tgtccggtca atcgtgggat gcgacggatc aggcggagat
     6601 cgatctgggg gtcacaacag gggtgccgca agcgagtggt ttcgtgttca tcgaggggcg
     6661 ttatctggct ccgccttata cggtgacgcg gaaagggaac ggtcttttca ttaaccgcac
     6721 gctggtggag cggccggtgc cttggccgcc tgcggtcacg ccgcaggtgc cggggacgga
     6781 aaagcgtgtg gacggcgatg gggcggcaca gccacaagcc gaaccgcaga acgaggcacc
     6841 tcagatcggg gagcgaaaaa aagtcttgag cgtcgacgac ctgttcgacg atgaggattc
     6901 ggatgaagag acggtgccgc ttgcggaaga tgaggtgaag cgcccggttg ccgaggcaac
     6961 cgccgcggcc gggaatggct tgtctgctga agagatcgcg cggcgcaagg ccgagactct
     7021 ggcggtgctg gaacgactgc gcaaggggta tgaggtcgca ttgtcgcagg gcgaggtgtt
     7081 ttttttcagt cagcggcaca accgggtgaa cggcacgtac gggacggccc gtctgttgat
     7141 gggcgtgctg ccagcggccc tgcggtatgc gaagtcgccg caggatttaa tgagtcggct
     7201 ctatcagggt ggcatcacgt ttattgatct caccatgtgc gccgcgttat acggccaccg
     7261 taacacgttt ccgctgctgg aggcgcggct ggcccggatt gaggaggcag aacgtgagga
     7321 ggagcggaag cgtaacgcgg tgcggcgccg gtaacgggag gagtggaaaa tggccagagt
     7381 gaaacagaag cgggacggaa aatcctttcg ggcggtggcg cgcgtattgg cggagatcaa
     7441 ctctaccgag gagatggccg cttttcttga tgagctgttg actcccgcag aggtgtacga
     7501 catctctctg cgatggaagt tgctcgggtt gcttgcgcag ggcatgtcgc agcgtcggat
     7561 tgccgaggaa ctgagaatca gcttgtgtaa aatcacgcgt ggatcgagaa tcctcaaaca
     7621 aaaaggggcc gtgagcgccc gtctgctgtc cgacgggaaa accgatggaa cggcatacgg
     7681 caagacgggc gggctgaaac ggaatatccg gaaaggggtg ggcggatgca agtgatcgca
     7741 aaggcggtgc aggagcagat gggtagcgcg tcttggattc ggaagatgtt cgaaaaaggc
     7801 gcggaactca aaaaggcctt cggggacgaa caggtgtgcg atttcagtct cggcaacccg
     7861 gacgtgccgc cgccggccaa ggcgcgtgac gcgctgtgtg cgctggcgga tcaggcggtg
     7921 cggccgtacg ggctgggata ttgcccgaac gcggggctgc ccgcgtttcg tgaggctttg
     7981 gcagacttcc tcgcgcggca gcaagatgtg ccgttgcggg cggagcaggt ggtggtcacc
     8041 tgcggtgcgg ccggcgcact gacctccttt ttccgtgcgg tgttggagcc gggcgacgaa
     8101 gtgatatgcc ccgcacccta tttcgtggag tacggcgcgt acggtggcca tttcggcggc
     8161 gtgctcaggc cggtgcccgc cctgccgccg gatttccagc cggatgtcgc ggcgatcgag
     8221 gctgcggtga cggagaggac gcgtgtgttg ctgatcaact cgccgaacaa ccccacgggt
     8281 tgcgtgtata cgcgggagac gctcgaacgt ctcggcgctt tggtggatcg ggtcaacgcc
     8341 gcgcgcgagc ggccgctctt tttggtgagt gacgagccgt accggttttt cgcgtacgac
     8401 ggggtgaccg tgtctccggt gctgccgttc tcgccctttt cccttgtggt cggatccttt
     8461 tccaagagcc tctcgttggc tggcgagcgt atcgggtatc tcgcggccaa ccccgccatg
     8521 ccgcaggtca agacgctgat ggacgcggtg accatgacca gccgtacgct ggggtttgtg
     8581 aatgcgccgg tattgggtca acggctggcg atggcgttgc tggaggaggg cgtgaacctc
     8641 gatgtgtatg accggcggcg tcaggcgatg gccgcggtgc tcacgcaggc ggggatccgt
     8701 ttcgccatgc cgcgcggggc gttctatttc tttccggaag cgcccggcgg ggacgatctg
     8761 gcgtttgtgc agcggttgct ggagcgtaat gtgctggtcg taccgggtcg cggctttggc
     8821 tttccgggtt atttcaggtt gagtttctgt gtggaggagg cggtgattgc gcgttcggcc
     8881 gaagggttcc aagccgcggt cgcgggctga ggctacgagg acgttgccga aaggagggag
     8941 catgcggaca aagttcggaa tcatgcgggt gacggtggcg gtgctctggg catgcggtgt
     9001 gcgggccgca gaatataccg ggcctgcgga gtggcggatg gtcaacacca acggggtgcg
     9061 ggcgtggcag gccgcacagc aggcggccgc ttccggcggc accgtcgcgg tctggccggg
     9121 tgtcgcggcg gatcctgtcc ggcgcgaggt gcggttgctg gctgaagccg tgggccacac
     9181 ggagggcgtc acaacggaat tcatcctgat cgggccaatg agtgaccgtg cctatgaggc
     9241 ggtggccgtg accgtggccg cgcccagcga tatcgtgcgg gctgtcgagc atctcggtct
     9301 gaagcgtggc ggaggggtgg gcagtcgtcc cttccgcttc tggcccgtgg gggaacgcgt
     9361 gaccgcgacg gtgcggcggc tcgatggcac ggatgcggca gtgctgccgc tgcaaaccct
     9421 gatccgcgac gagggggagg gcgaaccgct ggtcggggag ggcggcctcg ttttcacggg
     9481 agggcgctgg gtcggtccgt cctgcctcac ggacaccaac atgccttgtt cggtcatatc
     9541 gctctacaat gcgggggaga cggttttcga cgtgccgttc cgggtcgggc agagtgaggt
     9601 gtacggccgt ctgaaactgg cgcgctctta tccgtgcggt gcgttgatcg aggtcgtgct
     9661 gcgtccgttg gcggcggacg gtcggccgca ggttctgtcg ctgagggtga ccgcggagcc
     9721 cggtgagggc ggggtgtcat tggttacgcg cggggcggac gggacggttc tgcatcgttc
     9781 cgctttggcc gaggggctgg tctggctacg gaaacaggcg gatacgggcc gtgaactttt
     9841 cgttacgctg gagatggatg agacgctcac gttgcaacag gctacggagg tcgcacggat
     9901 tttcgatatg ctggatggca aggggttgaa actcgacggc aaaggggaag acggtgttta
     9961 cgccaaggcc tttctgccgc tggagaggtg gcgcgagcgt gaggggcgca cgcctcagcc
    10021 ctttgaattg catgtgaaac gaaacgcgga gggcggctgg accagaagtc tggttttcat
    10081 cgaagaggac tggcaggtgg agggtctcga tccgcggctg accccgcgca cgtatccgtt
    10141 cgaggagtgg gaagcgctgc cgggtctgac ggcccgtgcg ggcggggccg agaataaggt
    10201 gcgggtgctt tttgttttcg caccccggga ggcgtcgctt cggacgttca tgcccggcgt
    10261 acgcgcgctg tcggggcggc tgccgatcgt gtacgtgttc ggcgaatgaa ccgccgtcct
    10321 atttgcggaa ttcctcccga agctgttcca gaaacgactg gaccttctgc aggttctcct
    10381 cgccgacagt ctgtttgatc aggccgccca acgcattgtg cagcacattc aggtcgaccc
    10441 gcggttgggt gaccgtcccg gtgatcggga tgctgatggt tgtgcccttc agcattttac
    10501 ccgcgcctcc ggtcagacgc tcggtgatgg gcacttgcag cagatagcgg atcgagccgt
    10561 cgaaggcgac ccagccgctg aacacgatag gctggttgcc caccaccatg cggatgggat
    10621 ccacatgcgt cttgccatcg cggatgacca gatgcaccgg cagatgctcg acggtgtagg
    10681 tctgctcctt gactttaagg aacgtcagca cttcacgtag cgcagggccg aggctcaggc
    10741 tcaggcggtc gaaacgcagg gagagatcgg cggagagtcc ggtcttcagg tccgtgccgg
    10801 gtcggtacgt gaaatggcgt acatccaagg tcaccgtgcc gtcccggatc ttgctcccgt
    10861 ggaacatcgg gttcagattc acaagcaggt tgtccaccat ctgttgggtg attttcacgt
    10921 tctccagcag acggctcttt tccggaacgg tcagcgatac gccgtccggg gtgtaccgca
    10981 gttgcggcac gaaaaccatc cgaccgccgt tcagcgtggg cgcgtatcgc gtgtcgagca
    11041 cccctttggc gaggcgtaga ttcacgtccg acggtccggc atccaacccc aggccttttg
    11101 cggaagcgag gaacgcggcg ccggaaaact ctccttcggc gaggagggtt gtcaaccctc
    11161 ctgcggtcgg agcggaaaaa cggaacggac gggctgcttt gcccgtcaag gtgaactcgt
    11221 cgattccgcg ggcgtccaaa aggcgcgtca cggcggcgaa atcgacggtc atgtcgcctt
    11281 gggcctgaag cagacgccgc accgcgagat cagagagcga tccgcgcgcg cgggcttcga
    11341 tcaggctcga acggatgtcg aaggtgtcga tccgcacgct ttggccgtcg gccgccagtg
    11401 tgaaatctgt cacgagccgc gcggccggtt cggcatagac agtctcgccg tcgcgttcga
    11461 atgccgcgct gccggtggcc gtggtttcgc aacgcaggcc gggctgctgc acgctttgcg
    11521 acagggtgcc ttccatgcgg agatccggca cgcgccagat gccggcggcg gtccgggcgg
    11581 caccttggcg caatgccgcg ttcatccgcg ctttgaccac accggaggcg gcttcagcgg
    11641 tggcgttcgc caccagtgcg ccctgccagg acgccagacg gcgcagggtc ggaggcggaa
    11701 ggagaccccc cagtgtttta cgcagggagt gcaggtccgt gtctgcggca agcgtgaagc
    11761 cacgcagcac ggtcggctga ccgctctgcg cgggacgcca gcgtttccat ccgcccgcga
    11821 gtttcgtctt gtccagaatc acgtcaaacg tcgtgtcttg taactcccgt tcgggctggt
    11881 cgcctttcat ggggacgcgg ctgttgagct tgagtgtgcc gagcggcacg gtcaggcgct
    11941 ggccgggacg gacttccact gatgtatcgg aaactttcag gaatccggtg accgccgcgt
    12001 gacccttctc gcgccgggtg gccagatcca gataaatgga accgaccatg ggcgggaccg
    12061 agtgcaggat acgtttgaaa tcgcgtgaaa accgggttag gttcacttgg cctttcacca
    12121 cggcggagtc gaagcggccg ctgccgtaaa gttccgcgaa cggggctttg agccgcaggg
    12181 tctccaccgt aggcccgtgc ccgtagggga aggatgcctt caggtgcagc gaaggttcgg
    12241 gcttcaggat cagcggttgc ccgtccacag tcaggttcaa gtccgcgata gcgagcgccg
    12301 catccaggcc cagcgcggtc tcgtcgccct gaaccgccac ggtaccctgg agcacgccct
    12361 gacgcacacg caatcccttg gcgaggttga gcgccggacc aaagtcccgc gccacagccg
    12421 cgagatcgac cgcgattttg gcgcggaggg ttccgatagg gacggttttg ccctgtccag
    12481 cggtctgcaa cgcgcccgat gcctcggccc ggacccacgg cgcggagagg tcgaaacggg
    12541 tgatggtgag ggagggacgc tcatacgtga gatccgtcat cagcgccaga tcgcccgggg
    12601 gcgaggggcg gtgtcccgca gcggcgactg tgagccgcgt gaccaacacg ccgcctttca
    12661 gggtcaggtg gtcgaggttc gtcatcgtta ccgtgaaggc gccttccgcc tttccgccgc
    12721 tgacccattc ctgaccggcc cactcacgca gcagtgggcg cacggcggtg agatccacgc
    12781 cgagcagttt gacggtcagc gcttccgctt cggcaggtgg cgcgccgcgg gcagcggtca
    12841 acgcccgcag actttgcacg cgtcccccga tgccgagcgt gccgccgccg atccgcatct
    12901 gcccgttgat ctcaatgggt ttgcggaacg aggtcagggt gatctggcct tcgatctgtt
    12961 gcgcctcgaa cggtgtttcg ctctggccgt tcacccgcag cgatccctcc cggatcacgg
    13021 catgcgccgc gatatccaga atcggcagga agaaaccgcc gtcgcgggag cgggagggct
    13081 cccgcggctt cgccgcgtcc gcacgggggc gtggcatgag gtccagagtg acctctggtt
    13141 tctctagcgt cagatttccc gcgttccatg tgcccagcgg cagcaagcgc cagagaccgc
    13201 ggtcggaagt caggcgttct gcggtgacgg tcatgccgcg ctcgggatca atcaggctga
    13261 cctgttcaag aaccggggca cggaaccacc cccacgacca cttttcaacc gtgaggcgga
    13321 tgtcgcctgc cgcgttcagg cgatcaagca cgaaacgtcg tcccgcatcg gttgagagca
    13381 gcgtgggcag cagcgccaca aacaaaaaaa cggggccgag aaagagcagg atcatacgga
    13441 ggagaattag catacaccct ggccgatgcg ctagggtctc ttccgtgtcg tcgtttcgga
    13501 tctccgtgaa atccatttcg ttgcctttcc ggttcgcgcg gggcgctcat aagatcggga
    13561 gaagcatatc atgttcacgc ggtctcgtcg atgccgtcac cagagttcgc cgggacgttg
    13621 gcagaagggg cggacttcgg tgatgtcgga tgcgtcgcag gccagcatga tcagccggtc
    13681 gatgccgagc gcgatccctc cgcagggggg catgcccgaa cgcagggcgt cgagaaacgg
    13741 ctcgtcaagc ggatagatca ctttgccgag ctgacggcgg gcttcggctg cggcgagaaa
    13801 ccgtgcgcgc tgggtttcgg catcgcagag ttcgctgtac gcgttggcga gttcgagacc
    13861 gccgatatag agttcccagc gttcggccac ctgcgggtca tccggcttga ggcgggcgag
    13921 cgaggcggcc tcagcaggat agtcggtcag cacggtgggc cggtcgcgcg gcagggcggg
    13981 ttcgacggca tgcaccatat cgaggtcgaa tcgttccgga tcccagtcac gtaccggatc
    14041 ccaaccagcg aaacgttggt aggcctcgcg gacactcagg catgtccacg ggggttcgag
    14101 atcgatttta accccttggt aaaccagagt tgtccggccg aacacggatt ggaacacctg
    14161 cgtcagcaac gcttccgtgt cccggaggat cgcaatgtaa tccgcatgtg cgcggtacca
    14221 ctccaacagg gtgaactccg ggttatggcg tcgtccgcat tcgccttcgc ggaagcaggg
    14281 accgatctga tagagccgtg gtgaaccggc tgcgagcaga cgcttcagat gcagttcggg
    14341 ggaggcccgc agccatgcgc gtcctgcagg aggcacatcg atgtgcgttt cgagcgcggg
    14401 cgccggaatg cgtaccggcg tctccgcctc cacaaaatca tgggcgtgga aaaaagcgcg
    14461 cacagcatgc agaatgcggc tgcgcagggc gaggcgttcg ggattcatga tcaaccgttc
    14521 aatagatctt ctcgtattcg cctttgccga ggcgcttcag tttggtgaag cccgcggact
    14581 tagcgcggtc atcgagtttg gatttggaac ggccgatggc aggggctgtg atcacccggc
    14641 ggatggccgc gccacaggcg ggacaggccg tcaggcgcgc gtcgtggatc gattgcgtga
    14701 cgtcgaagct gccgacgcaa tggggacaac ccgcgtcagg atttacggcc tcgtagacat
    14761 agacaggcat gttgggtctc cggatgggtt ccgatgcgtc acactatagc ataatccggc
    14821 aggaaaagtt gaggtttcgg gtttcggatt ccagatttct cacacgaagg ttttttgggg
    14881 ggcgctagcg atgatgcgaa gaaagccctg accacgggaa acccgatcga gacgggtacg
    14941 cggctcgaaa gagggtgccc gcgcttgaat cgaagggggg cgcttgctaa gatgcggagc
    15001 ggaaagaagg acgaaagggc cccaaagcca aatgtgccga taagacagaa agagcgggat
    15061 gaaaacagtc aaggtcaatg gacagcggat ttcggaagag gcgattcagt ttgagttgga
    15121 gcggctcgtc cgtttccatg ccgaacacgg gatggcggag gatcaggtgc gtgcgcaact
    15181 gccggctctg gttgacaagg cgatcgatca ggccatcggt gcgaagctgc tgcttgatga
    15241 ggccagccgt ctcgacatac cggttcgcga ggaggatgtg gaggcgcaga tcgcccggat
    15301 catcgagcag gtcggagggg aggatgcatt ccgcaaggcc ctgaccggac aaaacacaac
    15361 ggaacaggtg ttccgcgacc agatccgtcg cgggtgccgg gtggagaagt tggtggagca
    15421 ggccgtggcg ggagtcgcgg aaccgaccga agaggatatc cggcggcatt tcgacgagca
    15481 caaggaggaa tatgctccag gcgagcgggt tctggctcag cacattctga tctcgccgga
    15541 cggtgacacg cagacctcga aggacgaggc ccgcgccaag attgaggcga ttcgtgagcg
    15601 cattcaggcc ggcaaatctt tttcggacga ggcggcagcg cactcgatgt gcccgagcgg
    15661 caaggacggc ggtagcctcg gctggttcag ccgcggcatg atggtgccgg agttcgattc
    15721 agccgccttt tcaatgaatg tcggtgaagt cagcgatgtc atcgagacgc agttcgggta
    15781 tcacatcatc ttcaaaaccg atcatgaggc cgcgggcgag gcggatttcg accaagtgcg
    15841 tgaaaagatc cgcgattttc tgcggcatgc ccgacgcggc gaggccatgg ccgcctatgt
    15901 caacgagctg aagtccaagg ccaagatcga ggtggagtga gcgagtgtga tggccaaaag
    15961 ggtataagga gaacgagatg aagaacatga gacgcctgtg ggtgacggtc ctctgcgtgg
    16021 ccgcaatgac gtacgctgaa ccggtgaagt tgattttcga caccgacatg gggaatgaca
    16081 ccgacgacct gatggcactg tgcatgatcc acacgttgca gtcgcgtggc gcggtcgatc
    16141 tgctggccgt gaccattacg aaagatcatc cgcaagccgc cgcgttcgtg gatgcggtca
    16201 acaccttcta cggacggccc gacattccga tcggagtggt caagggcggc gtgacgcccg
    16261 agcccggtaa attcaatctg ctggctgcag ccaagaacga ggacgggagc ttgcgttatc
    16321 cgcatgatct tacctccggc gagcaggccc ccgaagccac tggcctgatc cgcaaaatct
    16381 tggccgccca accggaccaa agcgtgtcgc tggtgcaggt cgggttcttc acgaacttcc
    16441 agcgcctgct ggattcgcct gccgacgcgc attcgccatt caccggcaag gagttgatcg
    16501 cgaaaaaggt caaggtgctc tcgctcatgg cgggttcttt caagccgatc aattggaaaa
    16561 cgcgccatct ggaatacaat gtgatcaagg atatccccgc cgcgcagaag ctggcgaaag
    16621 agtggccgac gccgatgatt tggagcggat ttgagatcgg gatcgcggcg gcctacccgt
    16681 atgagtctat cgaacgggat tatgcctaca tgaagcacca tccgctgccc gaggcctatg
    16741 cgctttactc ggcgaaaggt cacaaccggc cgacgtggga ccccacctcg ctgttggttg
    16801 cggtctatcc agaccgcggt tatttcacgt tctcaccggt cggcacggtg acagtcgaga
    16861 acgacggagg cacgttgttc gtgccgggca aaggacgcga ccggtatctc tcgatgaatg
    16921 cggagcaggc cgaacgcgtg cgcgaagcga ttgtgcagct ctgcgtagca ccgccgacac
    16981 atgccgccgc cctcaaaaac cgctaatcct gaaaagagca gttgatttcg ataccgatgc
    17041 tcctcgagtc tggcgaatgg agctggagtg cggtggcaag cggtattccg agcaacgctg
    17101 ctttgataca gaggggccgg gaaacggtgt cttgtcgccg tattcatgct tgaaaagcgg
    17161 ctcacgctca atccgcgcgt cccctgtcat caaccgattg cgcgagcgtc atcagtgcga
    17221 cggtagcgcg gccaaggctt gctgcaggtt tggatagaac atccggctcc ccggcagatc
    17281 atcatcccat ccggcgcgtc ggaccacgtc tctgacaccc gctttcatgc cggcgcaagc
    17341 gaactgcacg tcggcggcgg cgcagtcgcg catcagctcc gtcaacatcg acacggccac
    17401 gccgtcgatg tcattgacgc cggacatatc caacagtatc cagcgcacct caggccgttg
    17461 caccagcatc tcccggatcc ggtcttccac aaacccggca ttggcgaaat agaggctggc
    17521 ttcgaagcgc aggatcagca gccagggcaa tgtgaccgca tccggataac gttggatgtc
    17581 acggaactct tgttctccga cctgtcctag aatcgtcatt cccgggcggg cgctgcgcag
    17641 gacgaacagc agcaacgaaa aggcgatgcc gaccagtagc cctttgtcca tcccgaagcc
    17701 cagcgtgacc agaaaggtca ccatgaagca ccagccgtcg gcctgcttga ttttaaacag
    17761 gtgtcgcgct tccttgaaat cgatcaggcc gatgaccgcc gccagaatga tcgccgccag
    17821 caccgcattg ggcagatggg taaacaacgg ggtcaggaac aacagcgtca gcagaaccag
    17881 cgcggctgtg aagaccgacg ccagcggtgt gcgcgcgcca gcctgatagt tcaccgcggt
    17941 gcgggaaaaa ccgcctgcga ccgggtatcc ggagaacagc ccgcccgcca cattggccaa
    18001 gcccagtccg agcatttcgc ggttggcgtc gatacggtac ttttcacggg ttgcgatcca
    18061 cttcaccact gcgatggact ccatcgtgcc gataaagaca atcatccatg ctgcaggaag
    18121 caggttccgt atgccgttcc aggagatgtc gggcaagcgc cagaccggca ggccgctgga
    18181 gacctccccg accgtgcgaa caccgtactc ctccaggttg aatgcccaga ccagcaggat
    18241 ggcggccgcg ataatcggca gagtgactgg gaagcgccag cgtttgaagc gagccagaag
    18301 ctgaatcgcg actgcgccca aaccgatcgc aagcgtcggc aggtgcgtgt cagagagatt
    18361 acgcaccacg tccagcgcca gtgccagcac ggagtttccg cccgacaact tgacccccag
    18421 cagatgcccg aactggctga ggccgatcat catggcacct gcggacgtaa agccgctgac
    18481 cacggcgtgc gagacgaagt tgagcaggaa gcccgcgcgg cacaacccga gtgcgatttg
    18541 tatcgcccca accagtagcg ccaacatggc cgcccgaccg gttatcgttg tcgcgtcgac
    18601 agcggtgccg ctcggtcgtc cggcacaggc atcagccacc agcagcgaca cgatcgcgac
    18661 gggaccgacc gcgagttgcc gcgatgtgcc caagcacgca taagcaagca agggcacagt
    18721 cgaggcatat aacccgacca caggcggcaa tccagccaac atcgcgtagg ccatcgcctg
    18781 cggcaccaac atcaccgcaa caaccaatcc ggcgatgcag tcctttcgca agtcctgtcg
    18841 actgtaagtc cgcaaccagg tcacggcagg gtttctcagc ggtttttctg cgaggccttc
    18901 caggcggtca tgccgccgat caggctgaag actttcggat aacccatcga tttcaggata
    18961 ctgccggcga tgttgccgcg atagccggaa ccgcagacga cagtgatgtg acgggtcttg
    19021 tccagatcga aatcccccgc aatgattttg ccgatcggga tatgcacagc ctgttcgata
    19081 tgaccggatt cccattcgag gtcagagcgc acatccacga ccagatggtc agggtatttc
    19141 tcaaacaccg tcttcagact ctcgggagtg agttggggca gatgatccaa gggtttggcc
    19201 tgcgccgccc atccgctgac accgtcgccg agataaccga tcagacggtc ataaccagcc
    19261 cggcggtaaa gatacgcggc ttcctccgcc cgggcgggcg tgtccgcaac aacaatgagc
    19321 tcggtgtcag ccggaatcac catccctaac cagtttaccg acaatggcgt caaaccgata
    19381 ttcacggaac cccggatatg cgctgcgccg aatgcgctcg gcacccgtgt gtcgacaatt
    19441 ttcgctcccc gggccagcgc ttcctcaacc tgcttcacat tcagcagatt aaaaggtgcg
    19501 gctgcggcca acgaggcagg accgcgccgg ttcttgtcga ccatcaccgc gaagttatcc
    19561 ggacggactt gaaaccccgt agtcatgatc ttgtggaact gctcgaaaga catgttgttc
    19621 agcaacgggt tgttgcggcg ctcaaacccc agcgtggaca ccggcttggc actcatgccg
    19681 cggccgcaca gcgaaccctc gccgtgggcg ggatagactt ccacccagtc gggaaaacgg
    19741 gccagcttca cgtacaggct gtcgtacaga tttttgacct gctcctccaa caaatccgct
    19801 ccggctaaat caggtcgtcc gatgtcgccg atgaacagaa ggtcgcccgt cagcaacgca
    19861 aaaacgtcct ccgaccggct gtgatccgtc acagcgaaac tcaccgagtt cggcgtatgc
    19921 ccgaaggttt cgatcacttc caactctgcg gctccgaacc ggaaacggtc tccatcgtgc
    19981 aggtcctcat gcggatatcc cgcctcgaca ccggggtgta cataaatctt cgcgccggtc
    20041 cgcgccgcca gttcattgct gccggtgata tggtcggcgt gcaggtgcgt gtcgaaaata
    20101 tgcgtgatcc gcacaccgtg ttgctgtgcc gcccgcagat attgttcgac atgccgctcg
    20161 gggtcgacca cgcaggccgt gccggacgcc gggcaaccga gcagatagga gaaacacccc
    20221 agaccgtcca ctttgaattg ttgaaacaac ataccctgtc ctcctgatca atccgcgcca
    20281 cgggcgggct gcccgatggc aacgggttaa aaagcctcgc atccattcct gagcgggagc
    20341 tgtgacttgg ccgcttgtca caccaaagct gttttgggcg gtttccccga aaccgccgcg
    20401 gacgcctcgg ggaggcgtcc ctaccgacaa cttgaacgtt gagtgttgca gttttcctct
    20461 cgtttttgtc tggaactgcg tgcggccggc ttgcctgcca cacgcaggct gtcggtcaaa
    20521 acgagtccag attcttgtag cagaaccacc agtcgaacga ctcatactct ttgcggcgct
    20581 tctcgatatc ggctatgtcg gatggcggcg ggacaatgac cttgtctttg atcagctcat
    20641 tgttcggcca gcccgccggc acggcgacct ttttggagtc ggagacctgc agggccttga
    20701 ccgcacggac gacctcgtcg atattgcggc cgatctcctg cggatagtac aggatcaggc
    20761 gaatgacgcc ctcgggatcg accacaaaga ccgcacgcac cgtattggtg cccttgccgg
    20821 gatgcagcat cccgagcttc agcgcaatgg cgtcattggc ggcgataacg ggaaaatcaa
    20881 tgtcaacctt cagttcgctc ttgatccact cgatccactt gatgtgcgag aaaacctggt
    20941 cgatcgacat cccgaccagc ttgcagccca aggctttgaa ctggtcatag cgcttttgga
    21001 acgcgacgaa ctcggttgtg cagaccgggg tgaagtccgc cggatggctg aacagcacaa
    21061 accagctccc cttcaaatca cccggaatgt tcatcgggcc ttgtgtggtc ttgaccgaca
    21121 actccggaaa cttatcgccc aa
//
LOCUS       DAOSLE010000032        21076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2765_length_21076_cov_8.521146, whole genome shotgun sequence.
ACCESSION   DAOSLE010000032 DAOSLE010000000
VERSION     DAOSLE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 21076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21076
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2765_length_21076_cov_8.521146"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PK576_03790"
     CDS             complement(<1..283)
                     /locus_tag="PK576_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HOE60743.1"
                     /translation="MKTTKKKKRVPSASDTYALLNRELSWLEFNQRVLLEARNPKVPL
                     LERLKFLSITASNLDEFCMVRVGGLKMLVAAQIRRTDPSGMTPRQQLVAV"
     gene            complement(302..1897)
                     /locus_tag="PK576_03795"
     CDS             complement(302..1897)
                     /locus_tag="PK576_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE60744.1"
                     /translation="MKKNRNTAEEAAVPTMIAVIDIGATFIRMLIAEQRDAKEAYILE
                     KAVQSVPIGRDIIASRRISNATVEQCAQILKSFRKLLDEYGIPLESVTAVTTPTLDMA
                     DNSAVFLDRLANTSHIAFRLLDPGQIGYYYHLAFRRLGKGKDGSNHDKGDAVVLEIGG
                     LTCSMLCRKEGEIRFVQTYSLGSLRMRQQMEQGDFRPRQLVALADGRTHEIAEQLRNN
                     VDDVKAFKLFLMGREMRFAGARLHPDKNNAVELDPAAGVISVTVDELNALLKQIEKSS
                     TEEIVREWRVSYADAELIAPALSIAISVARAVGLKSVCISSLSFCHGLLEEAVDGTPW
                     TNTMRRHVLRVARATGRKYQHDVNHAEQVTKLSLQLFDLLQEEHGCTQRHRLILEVAA
                     LLHDIGAFVSAHSHHTHTMYLIRNTEFAGLSSEEIELIAVVARYHRKAMPKASHPEYS
                     ALSHEDRLVVSKLAAILRVADALDRVHDQSLGKIRFKLDETSLVCIPSRRVATSAEQV
                     ALAEKGDLFEVMYGRHCYLYDPK"
     gene            complement(2267..3079)
                     /locus_tag="PK576_03800"
     CDS             complement(2267..3079)
                     /locus_tag="PK576_03800"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOE60745.1"
                     /translation="MAKKTQQKRSSWDIVQIARHAERPNITDTIRLICDDFVELHGDR
                     LFGDDRGLIGGFATIGGVKIMLIGHRKGRGVEENIEANFGMANPEGYRKAMRLMRLAE
                     KYGLPVVTFVDTPGAYPGSDAEARGQAEAIAHNLEFMSVLKTPIVVVVTGEGGSGGAL
                     GIAVGDRILMLQNAVYSVISPEGCASILWRDGSLAAKAADTLKITSDALKKLGIIDEI
                     IAEPEGGAHTDPAATAAAMKRAVLKHLAELRTIPTEKLLKQRYAKFSAMGQF"
     gene            complement(3162..3992)
                     /gene="accD"
                     /locus_tag="PK576_03805"
     CDS             complement(3162..3992)
                     /gene="accD"
                     /locus_tag="PK576_03805"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOE60746.1"
                     /translation="MSWYERPVETPFRVPTEEVWVICPACKAYIGKSAWRAAEKVCPR
                     CNHHARLSCRERVALLTDAGSFKEINADVSFNDPLRFTDASGAYADKARATIAKTGEG
                     ESILTGTAAIESIPVVLGVMDFRFLGGSLGSGTGERIRLAAEHAIVTRRPLIIVSASG
                     GARMHEGIVSLMQMAKTCAAIARLKDAGLPYLSVLTDPTTGGVSASYAMVGDLNIAEP
                     KALIGFAGRRVIEQTIKQKLPDDFQTAEYTLAHGFVDCIVPRKELKAFLAKVLRYAGF
                     "
     gene            4209..5570
                     /locus_tag="PK576_03810"
     CDS             4209..5570
                     /locus_tag="PK576_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE60747.1"
                     /translation="MGRRGIRGVAAGCCLALLAASGCLTRDMVYSDLLETREEAYDAW
                     LRIHESGDIPIPSENLTLDEAVRIALLYNKTLQAKIQEREAARGQQLAAYRLALPRVD
                     AAAATESGTDDTESARLTLRQPLFRAGGTTAGLRAARLYAFWSDESVRNTAHSIIRLT
                     TADYYTIRLVENLCQVNRDAVVSAEAHLNDVRIKRVNGVASKYDELRAQVDVANFRAE
                     LIRQENNLVLARNSLLRILGTATDAQFRLADPLTYKPVSMTLEHALQMAYRNRPDLLM
                     AELNIRLQRENVRALRSTYFPEIDLALSAGTNRDRDGDDRWRDTHRAALELNWPLFDG
                     LAREGDLIAAKALLKQREIELQKAEEDVFLDVRQALLNLKNADEYVESQRLNLQHAEE
                     GLRLAMTGYREGVNTEVEIVDARSALTEARGNYYKALYDHCTARLALDHATGLLGQDL
                     ETP"
     gene            5567..6748
                     /locus_tag="PK576_03815"
     CDS             5567..6748
                     /locus_tag="PK576_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE60748.1"
                     /translation="MKTFAKLLLSLVVGIAIVAGARLAYQAAVRKAHPPASGKGQPSL
                     PVRIAEVRTGNVTNAIRLTGSLEATRVVDVMPKIAGRLERLALEDGTPVLEGLAVSNN
                     QIIAVIDHRDILAQMTQARAAVESARAAVATAKVVLKDRNRERQRMEKLFAEGSTTEQ
                     QRDLAVTAYEQSVTGLAQAEAHLTHAQTAVGVLDVTLSEAFIHAPMDGVISAKSVDAG
                     AMVNASTRIVQVMPLCELKFLIAIPGPYLHLLTTGKTVVSVRSDAVPDRVFEGLIARI
                     HPAVDPITRTATVEVRLANELGASGEWLLRPGLYAEGQIILEVKRDVVTLPADVALRR
                     GPRFLAFVVKDGHAETRELKIGARDGNVLEILAGLAVGEQVVIMGQHRLTDKIAVRIT
                     E"
     gene            6847..9963
                     /locus_tag="PK576_03820"
     CDS             6847..9963
                     /locus_tag="PK576_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE60749.1"
                     /translation="MRLPELGVKRPIFTLMVFIAILVLGGVSLVYLPVDLFPEIETPS
                     VTVITQWPGASVEDVEGRVTRVIENQLSIVNNLNEVTSRTREGVSAITCMFEWGTDLE
                     EAANDIRDRVSISRRFLPDDIEEPIIYKFNSSSMPVLTFAATAQESWEKLFDIIEDDL
                     GDALKRLPGVGAVNVYGGLHRQINIDIDPMKLAGYNLTLAEIEETIARENLTLPAGSI
                     KSGVIEYTVRVPGEFEDPEEARNIVVKRNASGGAIYLRDVADVYDGFEEPTRLSGADG
                     KQSVIFAVQKRSGANTLKVCDSVLQAVEEYARFLPQDIRIVNVMNSSDFIRQSLSNVT
                     GTLLWGGLFVTLTTFVFLRTFRASLVIVLTIPFSLIIAFFFMFIRGWTLNIISLSSLS
                     VAIGMVVDNAVVVLENIDSYTRRGVKLREASMYATGEVGLAISASTLTTVAVFVPLIF
                     VTGMAGIFFGQLGGIITTTLMASLFCSLMLTPMLATYLLKEDRDAIGGGWLGRTLYRL
                     SEAGLQAIEEAYTRLLVRALCWRGVICALAFAVFCFSLALFPLIGSEFSPDTDSGDLT
                     VNFELGLGTRVEETARLCEEVRQLGLDLIGAEHVVNSRWRCGDSKFGSGRQGSHIGRV
                     EFKLTPMDRRPFNVRDAGRLLLDTLRARPEFVKTSMSTVNRQMGRFSGSGQPIVIEIL
                     GHDLAQTRRVAEEIKKLCDRTPGARDASVSMDDGKPELLVKVDRLKVSTLGLNVSDVV
                     ETIRTLFYGKEASDFREGENEYWFFLRLKESHRRELANILDSEIALPNGRRVRVDAIA
                     DVVQDLGPLDIERKDQERMVKVEVDYHERSIGEVVASIREGIAKEIVLPEATRITYGG
                     MVKEQTEAFADLIGLTILGLVLVYMVMAAQFESLVDPLVIFLALPFAFSGVLFGLYGF
                     GMTFNMFSFIGIILLVGTGVNNGIVLVDHINILRARGEGLTDAICHSGRQRLRPVLIT
                     TLTTVFGMLPLALAGGEGSETWKPMGVTVIGGLSFSTIVTLVLVPVLYSLFNRDARNP
                     KTQRKDPR"
     gene            9960..10835
                     /locus_tag="PK576_03825"
     CDS             9960..10835
                     /locus_tag="PK576_03825"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOE60750.1"
                     /translation="MKKCVWMALATLLLTSAVEAQILKPVPPADVQTLREAIGTRMTA
                     QPSKPRTVLVFWRCEGFIHTEAIEYGNKAFEIAAQQTRAFRVEFSSDYRALTDTNKLQ
                     RYDAVVLNNTTHLKTKDHPALVPNLTAFVKSGRGLCVIHAGADNFYNSPEAAEMVGGL
                     FDGHPWGGGGIWAFKLDEPGHPLNRAFGGQGFKIGDEIYQQKSPPYDRSKLRVLVSLD
                     LSDAKTASAKGQKRADKDFAVSWIRPYGAGRVFYTSFAHDRRAYLNKATLWHILDGLQ
                     YTLGDLKAEDTPVPK"
     gene            11108..12871
                     /gene="ilvB"
                     /locus_tag="PK576_03830"
     CDS             11108..12871
                     /gene="ilvB"
                     /locus_tag="PK576_03830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOE60751.1"
                     /translation="MSKTPPTGTGHDSAPTMKSGAQCVIDGLVHAGCEVLFGYPGGAV
                     LDIFNCLYDAPFRFILARHEQGAVHMADGYARATGKPGCCLVTSGPGATNTVTGLATA
                     HMDGIPLICLTGQVALNMIGNDAFQEADVIGITRPVTKHNFLVRDADDLPDILAKAFY
                     IATTGKPGPVVIDLPKNVQKQKTAAVPPETVSMRAYHPDVPINPAEIEQFAEMINQAE
                     RPLLYVGGGAIAGDAPQRIAELAHRANIPVCTTLMGLGAFPEDDPLSLRMVGMHGSVA
                     ANYAVDHCDLLISAGARFDDRVTGKISTFASRAKIVHIDIDRSSIGKSVQTDLGVQGY
                     LDPVLTAVLPLVKPAEHAAWLEQVNRWKSRFPFAYPTPEGVLMPQYVIEQIYEVSQGR
                     ALITTDVGQNQMWATQFFRYTRPRSFISSGGLGTMGFGLPASIGVQIGRPDEQVICIT
                     GDGGAQMNFQELVVAVEHNLPLVVVILNNGYLGMVRQWQELFYGKRYAGVMLSQHGRL
                     PNEGIDENPVYLPDFVKLAEAHGAKTCRITRPDEVAPALRQALEERTTWVIECIVAPE
                     ANVFPIIPPGQHVDEMINRTV"
     gene            12938..13438
                     /gene="ilvN"
                     /locus_tag="PK576_03835"
     CDS             12938..13438
                     /gene="ilvN"
                     /locus_tag="PK576_03835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOE60752.1"
                     /translation="MKHIINCLVENQPGVLARIVGLISGRGYNIETLNVGPTADPTVS
                     KMTIVVPGDAHVIAQVTHQLSKQVDVIEVVNMTNRRHIDRELLLVRVATQNHIMRAEL
                     VDLAALFMARVVAVQEDSVTIQMIGNQETVSDFLRLLKPYTVIDISRTGTIAVGRESS
                     DPTVLK"
     gene            13449..14309
                     /gene="aroE"
                     /locus_tag="PK576_03840"
     CDS             13449..14309
                     /gene="aroE"
                     /locus_tag="PK576_03840"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOE60753.1"
                     /translation="MRTAYRFAIIGHPVKHSLSPLMHEASFRALGIEAEYRCIDVPPD
                     TLGDRLAACRAEGFNGLNVTVPLKELALPLMDHLDASARLYGAVNTIRLDARGMTGFN
                     TDAEGFLTDLKLGYRLSPLSRTVLILGCGGAGRALATACVREGASDVLLADLDASRVN
                     RLAEEIARLLPDAAARVRPLPADPAAWREPSLSADLVVHCTPLGLHEDDPPALPPDAF
                     RPGQFLYDIVYTSRVTPTMRAAQAAGADTANGTGMLVRQGAAAFTVWTGLTADVAAMH
                     AALESRLYGT"
     gene            14726..16300
                     /locus_tag="PK576_03845"
     CDS             14726..16300
                     /locus_tag="PK576_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60754.1"
                     /translation="MRKKRIAVVGLVGLLFATSWCAGNDVVAFKDADKIKSWDAWDDH
                     KAKAAVTNILQKEKRNVTWQKLNAVNPGLKEIGRLATRTSEQVKSSKWSVGCETMDRD
                     YADWDSFKALIPMLGVKHARFFSGWAKTEQEKGKYDFTWLDPQIRECAAMSVKPWVCI
                     SYGNPVWGSDFRLGMRLKPVTDNPDAFDAWIRYTKALVERYKDVVDEWEIWNEPFGQA
                     REYAELFYRTAKAIREIQPAAKCFCTAISWNARDPMKSDYAMVLEKLKKENALDLGSY
                     FLYHPYSPVPEASYGHLAEPLRKFVKGYSANFDIMQGEVGCPSQLEFGHAMNGIEWTE
                     YSQAKWNLRRAIGDSARSIPSSVFTMIDLKYTFMLQSFGLVRSNLLKEFVYRRPSWYA
                     MSNIYSLFDDETLPQKNLVLKNFAIQKRADPRDRKPHDLACTLFTRHGKPLRFYWFCE
                     ARPSDRLDFDQIDLMIPERLDQPVWVDMITGRVFAIPETQIDRQEESLVLKNIPMWDS
                     PVLITARTTVMLKDEG"
     gene            complement(16389..17390)
                     /gene="gap"
                     /locus_tag="PK576_03850"
     CDS             complement(16389..17390)
                     /gene="gap"
                     /locus_tag="PK576_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOE60755.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAVKNFSKEIEIVGINDLLDPDYLA
                     YMLKYDSVHGRFQGEVSVEGNNLIVNGKKIRLTSEKDPAALKWNEVGADIVVESTGLF
                     LTKETAEAHIKAGAKKVIMSAPSKDDTPMFVYGVNHKSYAGQAIISNASCTTNCLAPV
                     AKVLNDTFGIVKGLMTTVHATTATQKTVDGPSKKDWRGGRGILENIIPSSTGAAKAVG
                     KVIPELNKKLTGMAFRVPTSDVSVVDLTAVLAKETTYDEIKAAMKAAADGELKGVLGY
                     TEDKVVSTDFRGEAKTSVFDAEAGIMLDSTFVKIVAWYDNEWGYSNKVLEMAKVIAK"
     gene            complement(18036..20201)
                     /locus_tag="PK576_03855"
     CDS             complement(18036..20201)
                     /locus_tag="PK576_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60756.1"
                     /translation="MFRYAGTAAIASVILCVGVNAGMRPITDGHAEEWLCAGTWAGPA
                     GLTLLGIDADNEVRPCAGDVVPVKRFDETCWTNWTGIVRTDAGYVIAEKGGDFTWAKG
                     CSYAALYVKSDEAKTVDLEVTQSGYETRAFVNGVLVRETNRLWRKRKEKTVGITDQGN
                     ELTVDTEVGRLTSTLPIPLKAGVNFVMLKLYTRQQAGERVFFDAEFKDAAGLASDVND
                     PETDPVKHNALGQMYASVNVSAVANLPHPGEKLSVTTAIHYPWRKHKRPKSTKKDTRQ
                     EIVTPACPETFDAVVEQVVRDFDGRETARITYPITVPGTNMAALCTAGETGYYSVHTT
                     IKDTEGRRLFVFPADGFSVIGGTVSMRKRYRAGKMKTASCFYWVNGANSDGIFTWMDR
                     MGILHNVGGSASDTNIWSKAAAKGITMTADFLDPWGSENFIGKLAAVSNAAPYTRIFK
                     AWNEIDISRPQFKKTPELWTARTKMEYEAVKAIRPDAVYTGGSFVRVGNSDWFKGCLT
                     NGMERYVDVWDVHAYPKYPPKLDDRHVANSGNESGYGVETAYRSALGRENDKLFYLGE
                     CGARCSHGDDARRWQADTVAKLTAWAGSATNYLQIAHLVPWVKTSDGGDIPIAHFPAE
                     AALYTAVALTDGLPYERITTESKHIQAARFGSAVMAWTTHPGGEWFTFTPPEGELVTV
                     DVVGRVRPTTRTTDGCVSLHLSPSPVYLLSRTDYDRLTR"
     gene            complement(20215..>21076)
                     /locus_tag="PK576_03860"
     CDS             complement(20215..>21076)
                     /locus_tag="PK576_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60757.1"
                     /translation="LTREAQGGEISSYSENGHRYRVHTFTENGTFTMPAWGRVSLLVV
                     GGGGGGGNNGGGGGGGGGVLIVTNLVLSPGTYPVTVGTGGLGGGAGSANPIGGTTSGE
                     SGGASIFANYYVPGGGYGGCCNVNGGIGASGGGGGRSNQSEASVPNAGSGREEWGGFG
                     GCKGTYDSSNVNAYGGSGGGAGGIGTREVGGDGMACDFSGELKWYGGGGGGGHGSGGI
                     HPKGGLGGGGDGGIALSVAAVEGTPGTGGGGGGGGHSVHTAAAGAMGGSGVVIIRYEV
                     LLNGFLISIR"
BASE COUNT         4118 a         6610 c         6176 g         4172 t
ORIGIN      
        1 tgacggcgac aagctgctgg cgcggtgtca tgccggaggg gtcggtgcgg cggatttggg
       61 ccgcaaccag catcttgagc ccgccgacac ggaccatgca gaactcgtcc agattcgaag
      121 cggtgatgga gaggaatttc aggcgctcga gcaacggcac cttcggattg cgtgcctcaa
      181 ggagcacacg ttggttgaac tcaagccaac tgagttcccg gttcagcaac gcataggtgt
      241 ctgatgccga aggcacgcgt ttcttctttt tcgttgtctt catcgcatgg caccgtttgg
      301 gttattttgg gtcgtaaagg taacaatggc gaccgtacat cacctcaaaa aggtccccct
      361 tctcggcaag ggccacctgc tctgcgctgg tcgccacgcg gcgcgacggg atgcagacga
      421 gcgatgtctc atcgagtttg aacctgatct tgccaagact ttggtcgtgt acgcggtcga
      481 gcgcgtcggc gacgcgcaag atcgcggcga gtttgctgac gaccagcctg tcttcatgcg
      541 agagcgcgga gtactccgga tggctggcct tgggcatggc ctttcggtgg tagcgtgcga
      601 caacggcgat gagttcgatc tcttccgagg aaaggcccgc aaactcggtg ttgcggatca
      661 ggtacatcgt gtgggtatgg tgggagtgcg cactcacgaa cgcgccgatg tcgtgcagta
      721 acgcggccac ttcgaggatc aggcggtggc gctgcgtgca gccatgttcc tcttgcagca
      781 gatcaaaaag ctggagcgaa agcttcgtca cctgctccgc atggttcacg tcgtgctggt
      841 atttcctgcc cgtggccctg gcgacacgca gcacatgcct gcgcatcgtg tttgtccacg
      901 gcgtgccgtc cacggcctct tcgagcagtc cgtggcagaa agagaggctg ctgatgcaca
      961 ccgatttgag ccccacggcg cgcgccacgg atatggcgat actcagcgcc ggcgcgatca
     1021 actcggcgtc cgcgtaggag acgcgccact cgcgcacgat ctcctcggtg gacgacttct
     1081 cgatctgctt gagtagcgcg ttaagctcgt ccacggtgac ggagatcacg ccggcggccg
     1141 gatcgagctc aacggcgttg ttcttgtcgg ggtgcaggcg cgcacccgcg aaacgcatct
     1201 cgcgccccat caggaatagc ttgaaggcct tcacgtcgtc cacgttattg cgcaactgct
     1261 cggcgatctc gtgtgtgcgc ccgtcggcga gcgcgacgag ctgccgcgga cggaagtcgc
     1321 cctgttccat ctgctgacgc attcgcaatg agcccagact gtatgtctgc acgaagcgga
     1381 tctcgccctc cttgcggcac aacatgctgc aagtcaggcc accgatttcg agcaccacgg
     1441 catctccttt atcgtggttg ctcccgtcct tgcctttgcc gagcctgcgg aacgccagat
     1501 ggtagtagta gccgatctgt cccggatcaa gcaggcggaa ggcgatgtgt gaggtattcg
     1561 ccagcctgtc aaggaagacc gcgctgttat cggccatgtc cagcgtcggc gtggtcacag
     1621 cggtgaccga ctccagcggg atgccgtact cgtcgagcag ctttcggaac gacttgagaa
     1681 tctgcgcgca ttgctccacg gtggcgttcg atatcctgcg cgaggcgatg atgtcgcgtc
     1741 cgatgggtac cgactgcacg gccttttcga ggatatacgc ctctttggcg tcgcgctgct
     1801 ccgcgatcag catccgtatg aaggtcgcgc cgatgtcaat gacggcgatc atggtcggca
     1861 ccgccgcttc ttctgctgtg ttgcggttct ttttcatcgt gtcgatataa atgattttac
     1921 ttcagaaatg tttggattgt gttagcttct ggttggtttg tgttcttgtg tcctgtggtg
     1981 aaacaatcga tcccgatagc gatcccaacc ccgaccccga tttggatgaa accacgaaac
     2041 cttgaaaccc tgaaaccaaa gagagtggcc ggatgagcag gtccgacgtc cggttctcgc
     2101 cgcaagcgat tgctgatgcg cgaagagact ggctaggact gctaacgccc aacaccgaag
     2161 acaaccccaa catcgcaggt cgaatgtcgc gggtcgcagg tcgacgttca cgcgaacggg
     2221 aacggggaaa cgatcccggt gccgtgaggg ccgtgcggcg cggctcttag aactgaccca
     2281 tggcgctgaa cttggcgtag cgctgtttca gcagcttttc cgtcggaatc gtgcgcagtt
     2341 cggcaagatg cttcagcacc gcgcgtttca tcgccgccgc agtcgcggcc ggatccgtgt
     2401 gcgccccgcc ctctggttcg gcgatgatct cgtcgatgat tcccaacttc ttgagcgcgt
     2461 cagacgtgat tttcagggtg tcggcagcct tggccgccag cgagccgtca cgccacaaaa
     2521 tcgaggcgca gccctccggg gagatcaccg aatacaccgc gttctgcagc atcaggatcc
     2581 ggtcgccgac cgcgatgccc agcgcaccgc cgctgccgcc ctcgccggtc accaccacca
     2641 cgatcggtgt cttcagcacg gacatgaatt caaggttgtg cgcgatcgcc tccgcctggc
     2701 cgcgcgcctc cgcgtcgctg cccggatacg cgccgggcgt atccacaaag gtgaccaccg
     2761 gcagcccgta tttctcggcg aggcgcatca ggcgcatcgc cttgcggtac ccttcgggat
     2821 tggccatccc gaagttggcc tcgatgttct cctcgacccc gcgacctttg cggtggccga
     2881 tgagcatgat cttcacgccg ccgatcgtgg cgaacccgcc gatcaggccg cggtcgtcgc
     2941 cgaacagccg gtcaccgtga agctcgacga aatcgtcaca gatcaggcgg atcgtgtccg
     3001 tgatattcgg acgctcggca tggcgcgcga tctgcacgat atcccaggat gaacgtttct
     3061 gctgtgtttt cttagccata tgccctaccg attaacaagt tagctggtct cgggccgctg
     3121 cgcgccgtcc gtcgcgtcgg gaggcgcgtc cgcggcgcgt gtcaaaagcc cgcgtaacgg
     3181 agcacttttg ccagaaaggc cttcagctcc ttgcgcggca cgatgcaatc cacaaagccg
     3241 tgggcgaggg tgtactccgc cgtctggaaa tcatccggca gtttctgctt gatcgtctgc
     3301 tcgatcacgc ggcgtcccgc gaacccgatc agggccttgg gctccgcgat gttcagatcg
     3361 ccgaccatcg cgtaactggc tgaaaccccg ccggtcgtcg gatcggtcag caccgaaaga
     3421 tacggcaggc ccgcatcctt cagccgcgcg attgctgcgc acgtcttggc catctgcatc
     3481 agcgagacga tcccctcgtg catgcgcgcg ccgccggatg cggaaacgat gatcagcggg
     3541 cgccgggtca cgattgcatg ctctgcggcc agccggatgc gctcgccggt gccgctgccc
     3601 agactgcctc ccaaaaaacg gaaatccatg acgcccagca ccacaggaat cgattcgatc
     3661 gccgccgtgc cggtgaggat cgactcgccc tcgccggtct tggcgatcgt ggcccgggcc
     3721 ttgtccgcat aggcaccgct cgcgtcggtg aaccgtaacg ggtcgttgaa actgacgtcg
     3781 gcattgatct ccttgaaact gcccgcatcg gtcagcaacg ccacccgctc gcggcacgac
     3841 agccgcgcgt gatgattgca ccgcggacag accttctccg ccgcgcgcca cgcactcttt
     3901 ccgatgtacg ccttgcaggc ggggcatatt acccatacct cttcggtcgg aacacggaac
     3961 ggcgtctcta ccggtcgctc ataccatgac attttcgctt actccttcac tcacccgaac
     4021 gctctcccgc caagccaacc cccggcggca cgtcccatac ggcctgttcc cgctttgatc
     4081 aaggtgacga taacattgtt tttatcattc tgacaagacc ggccctattt ttttattata
     4141 cactccacac aaaaccgtgt aacgtaatgg ggtttcaatg gggaagcgaa cgtttcggaa
     4201 cgggggatat gggacgaaga gggatcaggg gagttgcagc cgggtgttgc ctggccctgc
     4261 tggcggcatc agggtgtcta acgcgtgata tggtgtacag cgatctgctg gagacgcgcg
     4321 aagaggctta cgacgcatgg ttacggatac acgagagcgg cgacatcccg atcccatccg
     4381 aaaacctgac cctggacgag gcggtgcgta ttgcgctgct gtacaataaa acactccagg
     4441 ccaagattca ggagcgcgag gccgcacgcg gccagcagct cgccgcctac cggctcgccc
     4501 tgccccgggt ggacgccgca gccgccaccg aatccggcac cgatgacacc gagagtgccc
     4561 gcctcaccct gcgccagccg ctcttccggg ccggcggcac caccgcggga ctgcgcgccg
     4621 cgcgcctcta tgccttttgg tcggatgaaa gcgtgcgcaa caccgcccat tccatcatcc
     4681 gcctcaccac cgccgattat tacacgatcc ggctcgtcga aaacctctgc caagtcaacc
     4741 gcgacgccgt ggtctcggct gaggcgcacc tgaatgacgt gcgtatcaaa cgcgtcaacg
     4801 gcgtggcctc gaaatacgat gagctgcggg cccaggtgga tgtggcgaac ttccgcgctg
     4861 aactcatccg tcaggagaac aacctcgtgc tggcccggaa cagtctcctg cgcatactgg
     4921 gcaccgcgac cgacgcgcag ttccggctcg cggacccgtt gacgtacaaa ccggtgagca
     4981 tgacgctgga acatgccctg cagatggcgt atcggaatcg gccggacctg ctcatggcgg
     5041 aactcaacat ccgcttgcag cgcgagaacg tgcgggcctt gcgcagcacc tattttccgg
     5101 agatcgacct tgccctgagc gcgggcacca accgtgaccg cgacggcgat gaccgctggc
     5161 gcgacaccca ccgcgccgcc ctggaactga actggccgct tttcgacggc ctcgcgcggg
     5221 aaggggatct catcgcagcc aaggccctgt tgaaacagcg ggagatcgaa ctccaaaaag
     5281 cggaagagga tgtgtttctg gatgtccggc aggcgctgct caatctgaaa aacgccgatg
     5341 agtatgtcga atcgcagcgg cttaacctgc aacatgccga ggaggggctg cgtctggcca
     5401 tgaccggtta ccgcgaaggg gtcaacaccg aggtggagat tgtggacgcg cgctctgcct
     5461 tgaccgaggc gcgaggcaat tattacaagg cactgtatga ccactgtacg gcgcggctcg
     5521 cgctcgacca cgccaccggt ctgctgggcc aagatttgga gacgccatga aaacgtttgc
     5581 gaaactcctt ctctctctcg ttgtcggcat cgccatcgtc gcgggtgcgc gtctggccta
     5641 tcaggccgcg gtgcgcaaag cccacccgcc cgccagtgga aagggacagc cgtcgcttcc
     5701 cgtgcggatc gcggaggtcc gcaccggaaa cgtgaccaac gccatccgcc tgaccggctc
     5761 gctggaggcc acgcgagtcg tggatgtgat gccgaagatc gcgggacggc tggaacgcct
     5821 cgcgctggag gacggcacgc cggtgctcga agggctcgcc gtctccaaca accagattat
     5881 cgcggtcatc gaccaccgcg acatcctcgc ccagatgacg caggcccggg ccgcggtcga
     5941 aagcgcgcgc gccgccgtgg ccacggccaa agtggtgctc aaagaccgca atcgcgaacg
     6001 ccagcgcatg gagaaactgt tcgccgaagg ctcgaccaca gaacagcagc gcgaccttgc
     6061 cgtgacggcc tatgaacagt cggtcaccgg gctcgcccag gccgaggccc acctcacgca
     6121 cgcgcagacg gcggtcggcg tgctggacgt gacgctcagc gaagccttta tccatgcgcc
     6181 gatggatggc gtgatcagcg ccaagtccgt cgatgccggc gcaatggtca acgcctcgac
     6241 ccgtatcgtc caagtcatgc ccttgtgcga actgaagttc ctgatcgcga ttccgggccc
     6301 ctacctgcac ctcctcacca ccggtaaaac tgtggtcagc gtgcgctccg atgcggtgcc
     6361 cgaccgcgtg tttgagggcc tgatcgcccg catccacccg gccgtggatc caatcacccg
     6421 caccgccacg gtggaggtgc gcctcgccaa cgaactcgga gcctccgggg aatggctgtt
     6481 gcggcccggc ctctatgccg agggccaaat cattttggag gtcaaacgcg atgtcgtcac
     6541 gctgccggct gacgtggccc tgcggcgcgg cccgcgtttc ctcgccttcg tggtcaaaga
     6601 cggtcatgcc gaaacacgcg agttgaagat cggcgcgcgc gacggtaatg tgctggagat
     6661 tcttgcgggc cttgcggtcg gcgaacaggt ggtcatcatg ggccagcacc gcctaaccga
     6721 caagatcgcc gtgcgtataa cggagtgagc cggtgatgat ccctgccgca aacacgaaaa
     6781 cagaatacgt cccgaacccg ccgactcggc accggtccgg gatcccgcag gaggagcgtc
     6841 aacggcatgc gcttacctga actcggagtc aaacggccga tcttcacgtt gatggtcttc
     6901 atcgccatcc tcgtgctggg gggggtctcg ctggtgtatc tgccggtcga cctctttccc
     6961 gaaattgaaa ccccgtccgt gaccgtcatc acgcagtggc cgggcgcgag tgtcgaggat
     7021 gtcgagggcc gcgtcacgcg ggtcatcgaa aaccagctca gtatcgtcaa caacctcaat
     7081 gaggtgacca gccgcacccg cgagggggtt tccgccatca cctgcatgtt cgagtggggc
     7141 accgatctgg aagaggccgc gaacgacatc cgcgaccggg tcagtatctc gcggcgcttc
     7201 ctgccggacg atatcgagga gccgatcatc tataagttca acagttcctc gatgccggtg
     7261 ctgacgttcg ccgccacggc ccaggagtcg tgggaaaagc tgttcgacat catcgaggat
     7321 gatctgggcg acgcgctcaa gcgcctgccc ggcgtgggcg cggtcaatgt gtacggcggc
     7381 ctccaccgtc agatcaacat cgacatcgat cccatgaagc tcgcgggcta caacctgacg
     7441 ttggccgaga tcgaagagac catcgcccgc gaaaatctga cactgcccgc aggcagcatc
     7501 aagagcgggg tgatcgaata tacggtccgc gtgccgggcg aattcgagga tccggaagag
     7561 gcgcggaata tcgtggtcaa gcgcaacgcg tccggtggcg cgatctatct acgggatgtc
     7621 gccgacgtct atgacggctt tgaggagccc acgcgcctct cgggcgcgga cggcaagcag
     7681 agcgtcatct tcgcggtgca gaaacgttcc ggagccaaca ccctcaaggt ctgcgactcc
     7741 gtgcttcagg cggtcgaaga gtacgcgcgt ttcctgccgc aggatatccg tatcgtcaac
     7801 gtgatgaact ccagtgactt catccgccag tccttgagca atgtgaccgg aacattactg
     7861 tggggcggtc tgttcgtcac cctcacgacc ttcgtgtttt tgcgcacgtt ccgcgcctcg
     7921 ctggtgatcg tgctgacgat cccgttctcg ctgatcatcg cgttcttctt catgttcatc
     7981 cgcggctgga ccctcaacat catctcgctc tcctcgcttt ccgtggcgat cggcatggtg
     8041 gtggacaacg ccgtggtcgt gctcgaaaac atcgacagct acacccggcg cggcgtcaaa
     8101 ctccgcgagg cctcgatgta cgccaccggc gaggtcggcc tcgcgatctc cgccagcacg
     8161 ctgacgaccg tcgcggtttt cgtcccgctg attttcgtta cgggcatggc cggcatcttt
     8221 ttcggacagc tgggcggcat catcaccacc acgctgatgg ccagcctgtt ctgctccctg
     8281 atgctcacgc cgatgctcgc cacctattta ctcaaagagg accgcgacgc gatcggtggc
     8341 ggctggttgg gccgcacgtt gtaccgactc agcgaggcgg ggttacaggc gatcgaagag
     8401 gcctataccc gtctgctcgt ccgggcactg tgctggcgtg gcgtgatctg cgcgttggcg
     8461 ttcgcggtgt tctgcttctc cctcgcgctt ttcccgctga tcggctcgga gttctcgccc
     8521 gatacggatt ccggcgacct gaccgtcaat ttcgaactcg gcctgggcac gcgggtcgaa
     8581 gagaccgcgc gcctctgcga ggaggtgcgc caactcgggc tcgacctcat cggtgccgag
     8641 catgtggtca actcccgctg gcgctgcggc gactccaagt tcggttcggg ccggcagggt
     8701 tcgcacatcg gacgggtaga gttcaagctg acccccatgg accgccgtcc cttcaacgtg
     8761 cgggacgcgg ggcgtcttct gctcgacacc ctgcgcgccc gacccgaatt cgtcaaaacc
     8821 agcatgagca cggtcaaccg ccagatggga cgcttcagcg gcagcggcca gcctatcgtg
     8881 atcgaaatcc tcggccatga cctcgcccaa acgcgacgcg tggcggagga gatcaagaaa
     8941 ctctgcgacc gcacccccgg cgcgcgcgac gcgagtgtca gtatggacga cggcaaaccc
     9001 gagctgctgg tcaaggtgga ccgcctgaag gtctccaccc tcggcctgaa cgtctcggac
     9061 gtggtggaga cgatccgcac gctcttctac ggcaaggagg cgtccgactt ccgcgagggg
     9121 gagaacgagt attggttttt cctgcgcctg aaagagtcgc accgccgcga gttggcgaac
     9181 atcctcgatt ccgaaatcgc actccccaac ggccgccgcg tgcgcgtgga tgccatcgcc
     9241 gatgtggtcc aggatctcgg gcctctcgat atcgaacgca aggatcagga acggatggtg
     9301 aaggtcgagg tcgactatca cgaacgttcg atcggtgagg tcgtggccag catccgggaa
     9361 gggatcgcca aagagatcgt gctgcccgag gcaacgcgca tcacgtacgg cggcatggtc
     9421 aaggaacaaa ccgaggcctt cgcggatctg atcggcctga cgattctggg tcttgtgctg
     9481 gtctacatgg tgatggcggc gcaattcgaa tcgctggtcg accccctcgt gattttcctc
     9541 gcgctgccct ttgccttttc tggagtcctt ttcggcctgt atggcttcgg catgaccttc
     9601 aacatgttca gctttatcgg catcatcctg ctggtgggca ccggggtcaa caacggcatt
     9661 gtgctcgtgg accacatcaa catcctgcgc gcgcgcggcg aaggcctgac ggacgcgatc
     9721 tgccacagcg gccgccagcg gctgcggcct gttttgatta ccaccctcac cacggttttt
     9781 ggtatgctcc ctctcgcttt ggcaggaggc gagggctcgg aaacctggaa acccatgggc
     9841 gtgaccgtga tcggcggact ctccttctcc accatcgtca ccctggtgtt ggtcccggtg
     9901 ctctacagcc ttttcaaccg cgatgcgcga aaccccaaaa cccaaagaaa ggacccaaga
     9961 tgaaaaaatg cgtctggatg gctctggcca ccctgctcct cacctcggcc gttgaggccc
    10021 agatcctcaa accggttccg cccgctgacg tgcaaaccct ccgcgaggcg atcgggaccc
    10081 gcatgacggc gcaaccgtcc aaaccgcgca ccgtactcgt gttctggcgg tgcgagggct
    10141 tcatccacac cgaagccatc gaatacggca acaaagcgtt cgagatcgcc gcgcaacaga
    10201 cgcgggcctt ccgtgtcgag ttcagcagcg actaccgcgc gttgaccgat accaacaagc
    10261 tgcaacgtta cgacgcggtc gtgctgaaca acaccacgca tctgaaaacc aaggaccatc
    10321 ccgcgctcgt gccgaatctg accgcattcg tcaagagcgg acgcggcctg tgcgtgatcc
    10381 acgccggcgc ggacaacttc tacaacagcc cggaagccgc cgagatggtg ggcgggctct
    10441 ttgacggcca tccttggggc ggcggcggca tctgggcgtt caaactggac gagcccggcc
    10501 acccgctcaa ccgtgccttc ggcggccagg ggttcaaaat cggcgacgag atctatcagc
    10561 agaaatcccc gccttacgac cgctccaaac tgcgcgtgct ggtgtcgctc gacctgtcgg
    10621 acgcgaaaac cgcctcagcc aagggccaga aacgtgcgga taaggacttc gcggtgagct
    10681 ggatccggcc ctacggcgca ggccgcgtct tctacacctc gttcgcgcac gaccgccgtg
    10741 cgtatctcaa caaagccacc ctgtggcata ttctggacgg cctgcagtac acgctgggcg
    10801 acctcaaggc ggaggacacc ccggtcccaa agtgaagaca aaaaaagacc cgccccctct
    10861 tgacagcatc cgctcttcag gataaagttg cgcaaatgag ttcatggaag tccaaagcgt
    10921 ttggggtgaa ccgcctctct gttgccgggc gggttacgcc cgtttatacc gtatcatggc
    10981 cgttcgcggc gggcttctaa aggaatcagg caacacgcga tttctgtttc aggacaagac
    11041 ctgccgttgg agagacggca ggttttttca tttgagtgag acgtaagcaa ggacaagaga
    11101 ggtgaacatg agcaagacgc cccccaccgg gacaggacac gattccgcgc cgacgatgaa
    11161 gagcggcgca caatgcgtca tcgacgggct ggtgcatgcg ggttgcgagg tgctgttcgg
    11221 ctatccgggc ggcgcggtgc tggatatctt caactgcctc tacgatgcgc cgttccgttt
    11281 catcctcgcg cgccacgagc agggtgccgt gcacatggcg gacgggtatg cccgcgcaac
    11341 cggcaaaccg ggttgctgcc tggtgacctc agggccgggc gcgaccaaca ccgtgaccgg
    11401 cctggcgacc gcgcacatgg acggcatccc gttgatatgc ctcaccggcc aggtggcgct
    11461 caacatgatc gggaacgacg cgttccagga agcggacgtg atcggcatca cgcggccggt
    11521 gacgaagcac aacttcctcg ttcgggacgc ggatgacctg ccggatattc tcgccaaggc
    11581 gttttacatc gcgaccaccg gcaaaccggg acccgtggtg atcgacctgc ccaagaatgt
    11641 gcagaagcag aaaaccgcgg ccgtgccgcc tgaaaccgtg tccatgcgcg cctatcatcc
    11701 ggatgtgccg atcaacccgg cggagatcga gcagttcgcc gagatgatca atcaggccga
    11761 gcgcccgttg ctctatgtgg gcggcggcgc gatcgcgggc gacgccccgc aacggatcgc
    11821 cgaactggcg caccgcgcga acattccggt gtgcaccacc ctgatggggc tgggcgcgtt
    11881 tccggaagat gacccgctct cgttgcgcat ggtgggcatg cacggcagcg tggccgcgaa
    11941 ctacgcggtg gatcactgcg atctgttgat ttcggcggga gcgcggtttg atgaccgcgt
    12001 gaccggcaag atctcgactt tcgccagccg ggcgaaaatc gtgcatatcg atattgaccg
    12061 ctcctccatc ggaaaaagcg tacagaccga cctcggcgtg caggggtatc tggatccggt
    12121 gctgaccgcg gtgctgccgc tcgtcaaacc cgcggaacac gccgcgtggc tcgaacaggt
    12181 taaccggtgg aaatcgcgtt tcccgttcgc ctatccgaca cccgaaggcg tgctcatgcc
    12241 gcaatacgtg atcgagcaga tttacgaggt gagccagggg cgcgcgctga tcaccacgga
    12301 tgtgggacag aaccagatgt gggccacgca gttcttccgc tacacgcggc cgcgcagctt
    12361 catctcctcg ggagggctcg gcactatggg gtttggcctg ccggcctcca tcggcgtcca
    12421 gatcggacgt ccggacgaac aggtgatctg catcaccggg gacggcgggg cgcagatgaa
    12481 tttccaggag ttggtcgtgg cggtcgagca caacctgccg ctcgtggtgg tcatcctgaa
    12541 caacgggtac ctcggcatgg tgcggcagtg gcaagagctg ttttacggca agcggtatgc
    12601 cggggtcatg ctgagtcagc atgggcggct gcccaatgag gggatcgacg agaatcccgt
    12661 ctatcttccc gattttgtga agctggccga agcgcacggc gcgaaaacct gccgcatcac
    12721 ccggcccgac gaggtggcgc ctgccctgcg tcaggcgctg gaggaacgga cgacgtgggt
    12781 gattgaatgc atcgtggcgc cggaagccaa tgtgttcccc atcattccgc ccggccagca
    12841 tgtggacgaa atgatcaacc gaacggtgta agcggagggt gcctgcacag ccgcttgaga
    12901 cttgaatccg atctgcccag atgaaggaaa agacagtatg aagcatatca tcaactgcct
    12961 cgtggaaaac cagcccggcg tgctcgcgcg catcgtgggc ctgatttccg ggcggggtta
    13021 caatatcgaa accctgaacg tggggccgac tgcggacccc acggtgtcga aaatgacgat
    13081 cgtggtgccc ggagacgcgc atgtgatcgc ccaagtgacc caccagctct cgaagcaggt
    13141 ggatgtgatc gaagtggtca acatgaccaa ccggcgtcac atcgaccgcg aactcctgct
    13201 ggtgcgcgtg gcgacccaaa accacatcat gcgggccgag ctcgtggacc tcgcggccct
    13261 gttcatggcg cgcgtggtgg cagtgcagga agattcggtg accatccaaa tgatcggaaa
    13321 ccaggaaacc gtgtccgatt tcctgcgcct gctcaaaccg tacacggtca tcgacatctc
    13381 gcgcaccggc acgatcgccg tgggccggga gagttccgat cccaccgtcc tcaaataaag
    13441 gagactgcat gcgcaccgca tatcgattcg cgattatcgg gcacccggtg aagcactcgc
    13501 tctcgccgct gatgcacgag gcgtcgttcc gcgcattggg catcgaggcg gagtaccggt
    13561 gcatcgacgt cccgccggac acactcggcg accgcctcgc cgcctgtcgt gcggagggct
    13621 ttaacggcct caacgtaacg gttccgctca aagagttggc cctgcccctg atggatcacc
    13681 tcgacgcctc cgcccgcctt tacggcgcgg tcaacacgat tcgactggac gcgcgcggca
    13741 tgaccggatt caacaccgac gcggagggct tcttgacgga tctcaagttg ggataccgtc
    13801 tctcgcccct gtcgcgcacc gtcctgatcc tcggatgcgg gggtgcgggc cgggctctgg
    13861 cgaccgcctg cgtgcgcgag ggcgcttcgg acgtgctgct ggcggatctg gacgcgtcgc
    13921 gtgtaaaccg cctggctgaa gagatcgccc gcctgttgcc ggatgcggcg gcgcgtgtca
    13981 ggcccctgcc cgcggatccc gccgcatggc gggaaccgag tctcagcgcc gatctggtgg
    14041 ttcactgcac gccgctcggt ctgcatgaag atgacccccc ggcactgccg cccgatgcat
    14101 tccgtcccgg ccagttcctg tatgacatcg tctacacgtc ccgcgtcacc cccaccatgc
    14161 gggcagcgca ggcagccgga gcggatacgg caaacggcac aggcatgctc gtccggcaag
    14221 gcgccgccgc gttcacggtc tggaccggac taaccgctga cgtcgcggcc atgcacgcgg
    14281 cgctcgaatc ccgtctgtac ggaacgtgag ggcataagga gtgaattcct gaattcccga
    14341 acgcaatccg tcaaacagaa agacaccgta gtttttttgg tgcgttgacg atccgtcaaa
    14401 tactggacac cgttggcctt tttgctcttt agtgcagaat aggcgcggcg cttcgcgccg
    14461 acgtattcca ttgtggcttt ccgtgacatc tcgtttctca tgcgcgccaa ttctagcacg
    14521 gtgtccagta tttgacggat acgcttgatt tctgggcacc gcgattttgg ctacggtgtc
    14581 catgacgatg gcacaccgca ggcctaccgc gcaccgcacc aaccgattac cttgacgttt
    14641 cggatggaga atgacaccat ggcgtcacga ttttggaaca gcgggtttct tacgtcggat
    14701 caattttgga aaggaaatga gagacatgag aaaaaagcgg atcgccgtag ttggtttagt
    14761 tggtttactg tttgcaactt catggtgcgc aggcaatgat gttgtcgcat ttaaagatgc
    14821 ggacaagatt aagagttggg acgcatggga cgaccacaag gcgaaagccg cagtgacgaa
    14881 tatcctgcag aaggagaagc ggaacgtaac atggcaaaag ctcaacgccg tcaatccggg
    14941 tctgaaggaa atcggtcgcc tcgccacacg cacgtcggaa caggttaaat cgtctaaatg
    15001 gtcggttgga tgtgagacga tggaccgcga ctatgccgac tgggacagct tcaaggccct
    15061 catcccgatg ttgggcgtca agcatgcgcg gtttttcagt ggctgggcaa agaccgaaca
    15121 ggagaaggga aaatacgact tcacatggct cgatccgcag atccgcgaat gtgcggcgat
    15181 gagcgtgaag ccttgggttt gcatctccta cggcaatccc gtgtggggta gcgacttccg
    15241 cctcggtatg cgcttgaaac cagtgacgga caatccggat gcttttgacg cctggattcg
    15301 ctacacgaag gcgctggttg aacgctacaa ggacgtggtc gacgagtggg aaatctggaa
    15361 tgagcccttt ggacaggcca gggaatacgc cgaactcttc tatcgcacgg cgaaggccat
    15421 ccgtgaaatc caacctgcgg ccaagtgttt ctgcacggcg atctcctgga acgcacgcga
    15481 tccgatgaag agcgattacg cgatggtgtt ggaaaagcta aagaaagaga acgcgcttga
    15541 tctcggatcc tatttcctct accacccgta tagtcctgtt cccgaggcat cctacggtca
    15601 tcttgccgaa ccgctccgta agtttgtgaa ggggtattcc gccaacttcg acatcatgca
    15661 gggagaggtg ggatgcccgt cccaacttga gttcgggcat gcgatgaatg ggatcgaatg
    15721 gacggagtat tcgcaggcga aatggaatct gcgccgcgcg atcggcgatt cagcgcgatc
    15781 gattccctcg agcgtcttca cgatgattga tctcaagtac acgttcatgc ttcagagttt
    15841 cggcctcgtt cgctcgaacc tgctcaagga gttcgtctac cgccgccctt cctggtatgc
    15901 gatgagcaac atctattcgc tcttcgacga cgagacactg ccccagaaga acctcgtgtt
    15961 gaagaacttc gccattcaga agcgcgccga tccacgtgac cggaagccgc atgatctcgc
    16021 ctgcacgctc tttacgcgtc acggcaagcc cctgcggttc tactggttct gcgaagcgcg
    16081 tccaagtgac agacttgatt tcgaccagat cgatttgatg attccggagc gactggatca
    16141 acccgtgtgg gtggatatga ttaccgggcg cgtgtttgcg attccagaga cgcagattga
    16201 ccggcaggaa gagagtctgg tgctcaagaa cattcccatg tgggattctc cagtcctgat
    16261 tacggcacgc acgacagtaa tgttgaaaga tgaaggatga aggatgaagg gcttgaacgt
    16321 tggacgttca aaaacgccgc ccccgcatca gcgggaacgg cgtcgtttac atatccggcg
    16381 gagatcgctt acttcgcgat aactttggcc atctccaaca ccttgttgga ataaccccac
    16441 tcgttgtcgt accaggccac gatcttgacg aaggtcgaat cgagcatgat gccggcctca
    16501 gcgtcgaaga ccgaggtttt ggcctcgccg cggaaatcgg tcgaaaccac cttgtcctcg
    16561 gtatagccga gaacaccctt caactcgccg tcagcagccg ctttcatggc cgccttgatc
    16621 tcgtcgtacg tggtctcttt cgcgagcacc gccgtcagat ccacgaccga gacgtcggag
    16681 gtcggcacgc ggaaggccat gccggtaagc ttcttgttca gttcgggaat caccttgccg
    16741 accgccttgg ccgcgccggt gctcgacgga atgatgttct cgaggatacc gcggccaccg
    16801 cgccaatcct ttttggaggg accgtccacc gtcttctgcg tcgccgtcgt ggcgtgcacc
    16861 gtggtcatca ggcccttgac gatcccgaag gtgtcgttca gaaccttggc gaccggcgcc
    16921 aggcagttgg tcgtgcagga ggcgttcgag atgatggcct gaccggcata gctcttatgg
    16981 ttgacgccgt agacgaacat cggcgtatcg tccttcgacg gtgccgacat gatcaccttt
    17041 ttcgcgcccg ccttgatgtg cgcttccgcc gtttccttcg tcaggaagag gccggtcgac
    17101 tcaaccacga tgtcagcgcc gacttcgttc cacttcagag cggcaggatc tttctccgac
    17161 gtcaggcgga tcttcttgcc gttcacgatc agattattgc cttcaaccga cacttcgccc
    17221 tggaaccggc cgtgaaccga atcatacttc agcatgtaag ccagataatc gggatccagc
    17281 agatcattga tgccgacaat ctcgatctcc ttcgagaaat tcttgaccgc cgcgcgaaac
    17341 accatccggc caatacggcc gaaaccattg ataccaacct tgatcgccat gttttgtact
    17401 ccttcgttta ccttgagaat gccgtaacgg gtattcctct ttgcaggcat gtcaacccga
    17461 cgccggttac cggcccatac acgggaaaac ttgcctgcgc cccttgcatg gatgggataa
    17521 aaggataccc gttcgcatgg cgcgcgtcaa ttgtgaatgc gcgttgattc gctctttttc
    17581 catcgattgt acaagagcgg ggatccaacg caagcgcagc ccgtaactca atttcgtctc
    17641 acataaagga cacaagggtt tttcatgaca tcccattccc cttcatgtcc tcgtgtggga
    17701 aacaacttgc tttttttggc cttgtcggcc gataaggcac gaatggggtt ccgggcaggc
    17761 acggtttcaa tagccggact ccgcgtcacg ggtggatcac gtttgattta cgcccatacc
    17821 cgatcaggtt catttcacac cttgaaaggt gtgaaattga cccgccagaa accgggcctt
    17881 ccccgcataa tccgtgcgac taaaaaagcc aaactccagc atttcacacc ttgaaaggtg
    17941 tgaaattggg aaccggcgtg ttaccgggtc gcaccttatg gagtgggagt gttaaggaac
    18001 acccttctat cgcggtcaga aaggggcgtg cccgtttatc gcgtcaaccg atcatagtcg
    18061 gtgcgtgaga gcagatagac aggtgaaggc gagagatgca gcgatacgca tccgtctgtc
    18121 gtccgcgtag ttggacggac acgtccgaca acgtccacgg taacgagttc gccctccggc
    18181 ggcgtgaacg tgaaccactc tcctcccggg tgtgtcgtcc acgccataac ggctgagccg
    18241 aaccgagcgg cttgtatgtg cttcgattcg gttgtgatcc gttcatacgg caggccatcc
    18301 gtgagcgcca cagccgtgta aagggcggct tcggccggga aatgggcgat cgggatgtca
    18361 ccgccatccg acgtcttcac ccacgggaca agatgcgcga tctgtaagta gtttgtggcc
    18421 gatccagccc aggccgttaa ctttgcgacc gtatccgcct gccaccgtct ggcatcgtca
    18481 ccgtggctac accgtgcccc gcattcaccc agataaaaaa gcttgtcgtt ctctcgtcct
    18541 aacgcagagc gataagcggt ttccacgcca taccccgatt cgtttccgga attcgcgaca
    18601 tggcggtcat ctaattttgg cggatatttg ggatatgcat gcacatccca aacgtccaca
    18661 taccgttcca ttccattcgt gagacaccct ttgaaccagt cgctgttgcc aacgcgaacg
    18721 aacgaccctc cagtgtagac cgcatccggc ctgattgcct tcaccgcctc gtattccatc
    18781 ttcgtgcgcg ccgtccaaag ctccggagtc ttcttaaact gcggcctcga tatgtcgatc
    18841 tcattccaag ccttgaaaat ccgtgtgtac ggcgcggcgt tgctcaccgc cgcaagtttg
    18901 ccgatgaaat tttccgaccc ccaaggatcg aggaaatcgg ccgtcatggt gatccccttc
    18961 gccgccgcct tcgaccagat gtttgtgtcg gacgccgagc cgccaacgtt gtggagaatt
    19021 cccatgcgat ccatccacgt gaagatgccg tcggagttcg cgccgttcac ccagtagaaa
    19081 cagctcgcgg tcttcatctt ccctgcgcgg taacgtttcc gcatggacac cgtaccgcca
    19141 atcaccgaga agccgtccgc agggaagacg aagagcctcc gtccttccgt gtctttgatt
    19201 gttgtgtgga ccgaataata ccccgtctca ccagccgtgc aaagggctgc catgttcgtg
    19261 ccgggaaccg tgatcggata cgtgatgcgc gctgtctccc ggccgtcgaa atcacgcacg
    19321 acctgttcga caaccgcgtc aaacgtttcc ggacatgcag gagttacgat ctcctgccgt
    19381 gtgtctttct ttgtggattt tggccttttg tgcttccgcc acggataatg gatcgctgtc
    19441 gtcaccgaca gtttctcgcc cggatgcggc aggtttgcca ccgcggaaac atttacgctc
    19501 gcgtacatct gcccgagcgc attgtgctta acgggatccg tttccggatc gttcacatct
    19561 gaagcaagcc cggccgcatc tttgaattct gcatcgaaga atacgcgctc gcctgcctgc
    19621 tgccgggtgt agagcttgag catcacgaaa ttgactcccg ccttgagggg gatcgggagc
    19681 gtgctcgtaa gacgtccgac ctcggtatcg accgttagtt cattgccttg gtcggtgatg
    19741 ccgacggttt tttcctttcg tttccgccag aggcgattcg tctctctcac gagcacaccg
    19801 ttcacgaacg cgcgtgtttc gtatccgctt tgagtcactt ccagatcaac tgttttcgcc
    19861 tcgtccgatt tcacatacaa tgccgcatag gaacaaccct ttgcccatgt gaagtcgccg
    19921 cctttctctg cgatgacgta tcccgcatca gttcgcacaa tccccgtcca gttggtccag
    19981 catgtctcat caaaccgttt caccggcacg acatcacccg cacacggccg cacttcgttg
    20041 tcggcatcga tacccagaag cgtcagtccc gccggtcccg cccacgtccc ggcgcacaac
    20101 cactcctccg catgcccatc cgtgatcggc ctcatccccg cattaacgcc aacacacagg
    20161 atgacggaag cgatcgcggc ggtccctgca tatcgaaaca taagaagcct tccattatcg
    20221 gatgctgatc aaaaatccat ttaggagaac ttcgtaacgg atgatcacga caccgcttcc
    20281 acccatagcc ccggctgcag ctgtgtgcac cgaatgcccg cctccgcctc ctccgccgcc
    20341 tgtgcccggc gtaccctcta ccgctgcgac ggaaagcgcg atcccgccgt cgccgccgcc
    20401 gccgagacct cctttgggat ggatccctcc ggaaccgtgt ccgccgccac cgccgccgcc
    20461 gtaccatttc agctcgccgg agaaatcgca cgccatgccg tcaccaccga cttccctggt
    20521 tccgatgccg cccgcgccgc cgccagaccc gccgtatgcg ttcacattac tactgtcgta
    20581 tgttcccttg caaccgccga acccgcccca ctcttcacgt cctgatcccg cattgggcac
    20641 actcgcttct gactggttgc tccgtccacc gccaccaccg gaagcgccga tgccgccatt
    20701 gacgttacaa caaccgccgt atccgcctcc cggaacataa tagttcgcaa aaatcgaggc
    20761 tccaccagac tcgccgctgg tagtgccgcc aataggattt gcagatcccg cgccgcctcc
    20821 cagaccgccg gtgccgaccg taacgggata cgttcccggt gaaagaacga gattcgtaac
    20881 aatcagaaca ccaccgccac cgccaccgcc accaccgttg ttgccaccac cgccaccgcc
    20941 acccacgacg agcagtgaga cacggcccca cgcgggcatt gtgaacgtgc cgttctcggt
    21001 gaatgtgtgg acacgatacc ggtgcccgtt ttccgaatac gacgaaatct cgccgccctg
    21061 cgcctcccgc gtcagg
//
LOCUS       DAOSLE010000033        20944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2796_length_20944_cov_8.143903, whole genome shotgun sequence.
ACCESSION   DAOSLE010000033 DAOSLE010000000
VERSION     DAOSLE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20944
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2796_length_20944_cov_8.143903"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..2049
                     /locus_tag="PK576_03865"
     CDS             448..2049
                     /locus_tag="PK576_03865"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOE60758.1"
                     /translation="MLRVFLSGAALLLTCLSAFGAAARFTAPRNYPNIGLKLPGLDSA
                     RADPVPMPQAQAFLSVRGTVLSREDRFQPFELWYGNQCCAQWRDAAGNRLVLGRATHR
                     LPVFEEETVPRERFEQAMQDGSGSEGFRDRAALNEWVATFADTPVYDPEPLRLNAFSL
                     DEVLFYPCGEADKLVYAILPRRQGSAERPDWFCVTFHAPGASDPDALREHFEEAFLSQ
                     ISQPSRTEVDQGPQAEELDVRSAKGARPPDLPDHPVRREARKSVENYGDWWFAETEGT
                     IILSDADTDLGKRLIQTLQETLPPLWKAFAQLVPPLTRDRDVALLRLFQRRADYARYT
                     GPAYAWTGGMWMPGRRELVLPLEADPQVVLRTVRHESFHQYLSFAYGMVPAAPWFNEG
                     HACLFENARMDAEGRVILDEDTDRVFVLLDHLDTVLTLLPHLLHASYEEFYAGFSAGR
                     RLKYAMAWGLVYYLQKGAPTERNTPFKQILPTYADALALELDGAKATEAAFADVDMAV
                     FLDTFREFWLKRRGTAMRYDPLQQR"
     gene            2176..2346
                     /locus_tag="PK576_03870"
     CDS             2176..2346
                     /locus_tag="PK576_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE60759.1"
                     /translation="MAYQITDKCVACGLCKDACPVEAISEGTPIYTIDADKCIDCGAC
                     SGECPNEAIIAG"
     gene            2469..3242
                     /locus_tag="PK576_03875"
     CDS             2469..3242
                     /locus_tag="PK576_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60760.1"
                     /translation="MKQVVVLVAGLLAIIGPGWADVACPGDYPGHLQGIATDESGNLY
                     WSWTTFLVKTAATGTLLAKVPVPTHYGDLTWHGGKVYVAVNFGQFNKEPGKAKSWVCV
                     HDAETLALLSSNAVPEVVHGAGGIEWHNGHFFVVGGLPATHTANYVYEYTGSFEFVKR
                     HVIESGQTFLGIQTVCRMRDGTWWFGCYGKPAVVLKTDDRFTFCGKYRFNGAVGMART
                     PKNDELLIATNRLIAKRNSASVRPVKTETITARIQEDKH"
     gene            3347..4846
                     /locus_tag="PK576_03880"
     CDS             3347..4846
                     /locus_tag="PK576_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOE60761.1"
                     /translation="MRYAIVSDIHANFAAWRTVLADIADIRADQIICLGDMVGYGPQP
                     VEVLESVYRTVQVTLMGNHDAAVCGRLDPGLFLPHARAAVLRHRAQLSAAGIAWLERL
                     PLVLTAPTFRCTHGDFSDPSAFYYILDEQDACPSWRATQEPLLFAGHTHMPGIYVSGA
                     SGQPHFLEPRDFTLEPGKRYIVNPGSVGYPRTGPFRSSYCLYDDEAGTVVFRQLPFDD
                     EAYRAALREAGLENSAPWLERLTLHRYRPAVRVRFSFANPVTASQSGRVSARRYGATG
                     RRLARNLALGLLALGAVGVAGRLIFRAGHATAESAPVAMMPPFELPVRAAYPLMHSGG
                     NLLPPLPESVAPDRRLDGWRYALEDRDRQRFALGLRDGGLTLVVRHDGPRRFQLESPL
                     IDLAGTRITALRLTSRVRKPGPFEGSATIQLVTYTRLPHDLLQRGRTEQYELRTTRRR
                     LVPPGAERNVRIRLGRAVTHVRFRVEATFDGVLEIEQPALRAEQEGHLP"
     gene            4843..5706
                     /locus_tag="PK576_03885"
     CDS             4843..5706
                     /locus_tag="PK576_03885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE60762.1"
                     /translation="MIQRVSEEVWAFDCEWVPDLQAGRLLYRLPEDLPSQEVLRVMWE
                     QGGATPENPTPFLKTILCRVVSVATVIRRVSRNGEVRLFLQALPEAPDDPSQDEAHIL
                     RRFLVDGMAAHNPQLVGFNSRNADLRILTQRALVLGLPQPALCGRLAAKPWESLDVDL
                     MDRISGYGKTYAVSLNELATLSGIPGKLDTCGDDVCGLWHSGQRRKIVEYNCFDALTT
                     YLVWLRMAYFSGCFDDEGYRTEQRRLYTLLRERLLKPEDAYLRAYLDAWRDLKRRTGQ
                     LDDGVFPPDDQ"
     gene            complement(5700..6479)
                     /gene="lpxA"
                     /locus_tag="PK576_03890"
     CDS             complement(5700..6479)
                     /gene="lpxA"
                     /locus_tag="PK576_03890"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOE60763.1"
                     /translation="MASIHPTAIVDPKAELDTDVEVGPYATIGPNVRIGAGTVVQSHA
                     YVVGHTTLGKNCQVFPFACVGMKTQDLKYQEGDVTYVEVGDETVLREFSTVHLGTKPG
                     EVTRVGSHCLIMAYCHIAHGCVVGNHVIMANVTTLAGEVTVEDFVTIGGMVGVHQFCR
                     IGTYAMIGGATKVRQDCPPYMLTENVGSDTHVLGPNIVGLQRRGFSVETRSALKEAFK
                     LIYREGLNRTQALERIKYEVADLPEIQTLVAFYQNSRRGVH"
     gene            complement(6509..7600)
                     /locus_tag="PK576_03895"
     CDS             complement(6509..7600)
                     /locus_tag="PK576_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE60764.1"
                     /translation="MKILERYVLQAFLSAFALAWLILSFVMTIGLLVRITQLMIEGLP
                     PRAVGLFMLVGFPETLRLTLPLALLVSALLVFSRLSADNEISAMRACGVNLLAIMRWP
                     LVTGALCTLIGLYINNEIVPRSHEVRRNLRSVISVDVGLDLLESGRFITDFPKMTLWF
                     ARKEGNWIQDLIVFDHSQEGLTREVRADKALVTTNGTDIVLEMYKVRIDPIEVDRPGV
                     ASADRFRHVIPEAMKRRRYVRKEKDFHFNELRQAIRKVRANEKGLSRKALQRLLSIYR
                     TEYRMRYVYAFASLCFVMIGVPLGIRTHRKESTIGMAVSLVVSMAYYLCVILLQSLDK
                     YPALRPDILLWLPVGVCLTLATWLISKNL"
     gene            complement(7689..8960)
                     /locus_tag="PK576_03900"
     CDS             complement(7689..8960)
                     /locus_tag="PK576_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE60765.1"
                     /translation="MSKSNVILSTVAAVGILCLQVKAEERVDAPRWYVNPGVGMILPE
                     GNQAIDEGLNLNLRLGYDLSDHWSVETEASFAPNMTNNGRWTQKKQRIYGVGLDALYH
                     FDRYNRFDPFLSAGWAVYMADRNVFEEGSRSTLTGPRVGIGAMYHLTDNLSLRADGRA
                     LMSVDTCHEMQYTLDAGVVYRFGGSGGSSFGEGSSSVTNLPKDSDGDGLTDDEEAKLG
                     TDPFKADTDGDGLTDFEEVRTYKTDPLNPDTDFDGLSDGDEVKKYKTNPLDRDTDKGG
                     VADGHEVIEDKTNPLDPSDDLMLFEVYIQFDYDTTVIKPQYFSEIDQVVRVLQRNTKA
                     TAVIEGHADRRTRSSEKYNQDLSERRAQAVQSYMVGKGVAVQRLSAVGYGYSRPKVQP
                     DLVNGNPENRRVEIYIRGAGTNADKVKYVNP"
     gene            complement(9302..11008)
                     /locus_tag="PK576_03905"
     CDS             complement(9302..11008)
                     /locus_tag="PK576_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60766.1"
                     /translation="MTREELERCSSAVTLSDMEVFVFPELMYSLVLANIMSPCLWRWR
                     EDPWFKGIRKMKPYRRLLRLKQYIMDHYVFNLDLETWGLTHQHHELERFANFLAPEVI
                     AQSNALFGYQGDTYYFDIDIRTHFGLDKYASEIIPYWKTETIEAMNAFQFREGYAAGA
                     GECVSLAALYAAALYVVAGIPLEKVFLMATPLHSQNFVEVDDGVLINNRRLVTKAMWF
                     NGTQISKQARRALENERVTIVSHPTGWIHMLYPRATIDAEAYGRFATGLRAYLKTPLT
                     PEILGNFLRQNPQCRQCFVMRHPIHGVDRYLSLEQAFQFEQECPYKVTDATRDKLLAM
                     IPMETFAASCCPGKIVLNDIEAFVQERAIDLDRSEDLKALRTAIGDTCMSAAEMVEQL
                     QRFCHTEPRLPDAAGVSFTPPPPILLPVDLTREEVIARIEDLRQVNLTADLAFYAWRD
                     LTRTPYAPFVKAALERNPVSLAGAAEMSESDLASLLAAWPGDSIYEGPARLAQPDEVW
                     NFRRGDGLEKALLMANVLRHRGCGPLRLTSEEGRAVLSAGERTACAFASAKTPAEQVW
                     NL"
     gene            complement(11005..12705)
                     /gene="lnt"
                     /locus_tag="PK576_03910"
     CDS             complement(11005..12705)
                     /gene="lnt"
                     /locus_tag="PK576_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOE60767.1"
                     /translation="MITRLRKGWVKGAAAVASGLLMWAAFPPQAQVHGMWMALAPLFL
                     VIRRSRPREAFGWSWLCGMLFWLLTLSWFPAIIRNNGPWFLVLLGQGALAAWCALFLA
                     MTGAASARVWGWAGQTPGWRRVATVAVFDPLLWVGAEVVRGWLFSGFAWNFLGVSQVD
                     NTALIQVASITGVYGVSALLVMGNGAVASLLERMAEPFVARCRPVPRTTVPAYGWAGR
                     FARSIESFLPLVVVLAVWFWGMGRIRVWDREARRSGVYRIALIQPNAPCIFEVSDETV
                     RMQWEQLVSQSRLVGAAKPDLVVWPETAVMGSVPYDTDTMRVIREGATAANAPLLTGA
                     LEVERAGVSRVTPDGCRYYNAAWLVSATGETLGCYRKRHLVPFGEYIPGDKLVPWLQR
                     FAPTGVSCTPGRGPGPLHVARGAGRILSLGPLICFEDTIPALSRGAVRAGARLLVLMT
                     NDAWFSGSVEPLQHQQQAVFRAVENGVPLVRAANSGVSCVVDAVGRVKRLESGEAITD
                     FDGFLVMPVTVPEVPLRAPYTAWGDWVLGIPGAVLLATLLVVGAVRDRRARRREEDAE
                     "
     gene            complement(12715..13239)
                     /locus_tag="PK576_03915"
     CDS             complement(12715..13239)
                     /locus_tag="PK576_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE60768.1"
                     /translation="MSEPLLGLVTGCLFGFLMQKAGVLRFDRQVGALMFRDMTIIKFM
                     LSAILVGSVGLQLLAEAGQITMSPRTLNTGVIVGGLLFGAGWAVAGFCPGTAVGAVAE
                     GRWHALFVIAGMFAGAALYAEAEPWLKTSVQAWRDFGPLTLSQVTHLSPWYCIALLWG
                     VGTVTFVWCAYKKV"
     gene            complement(13232..13780)
                     /locus_tag="PK576_03920"
     CDS             complement(13232..13780)
                     /locus_tag="PK576_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE60769.1"
                     /translation="MISKKRERRWNPYLTGALTGLLAIASAYATTRVTGRTVYLGAST
                     TIVRATAEIERRILPGHASRNAYLKAHPGVWDWQAGLVCGIAIGALIAALSGRTFESE
                     WVPERWRKRFGPNVAKRAAGGFLGGAAALFGARLAGGCPSGLGLSGAMQLSVSGLVGM
                     ICFFTGGVFLIRLIGKRRDAYE"
     gene            13918..14277
                     /locus_tag="PK576_03925"
     CDS             13918..14277
                     /locus_tag="PK576_03925"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOE60770.1"
                     /translation="MYCTLLKAKLHHLRVTQANKEYEGSLTIDEDLMDAVGILNFEKL
                     LIANLDNGERFETYAIRGPRGSGVACLNGAASYKGQIGDRLIVMVWCLLDDTERAAHH
                     PKIVRLDDRNRIVATAL"
     gene            14470..14545
                     /locus_tag="PK576_03930"
     tRNA            14470..14545
                     /locus_tag="PK576_03930"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14503..14505,aa:Ala,seq:ggc)
     gene            14931..17075
                     /locus_tag="PK576_03935"
     CDS             14931..17075
                     /locus_tag="PK576_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60771.1"
                     /translation="MGQTWGRVIPLSISLLLGGCSGMGVAQKGTVLHVAPDGNDVWSG
                     RLERAAKGDGPLATLDGARRAVRALRTSGTLPPGPVTVLFAPGVYRQTAPLFFTEQDA
                     GTAASPVRYVARPGTEVIFSGGIVISPDWEVRDDAAAARLDAAARPHVRIYDIGNGRL
                     PEIAEPGQKGWFAPLWISENGIPLTAARWPNTGFVKIADATRSKKGSAPSPEANGFTY
                     AGDRPDRWVGEADPRVSGFLGNDWAAANVRVASIDPALKRITQGKPSSAYGFRKGGRW
                     FGYNLLCELDAPGEVYADLKAERLFVWPKTDTKNAEREVARCRALLILRETAHLTFEG
                     ITFTSALESGVEVRRSEDLGFTRCQFRSIAGTGLLMESVRRVRVAGCEFTHLGAGGLS
                     ASSGDAQQLTPGGLVVDNNHFAFFGLTSLSYAPALQLSGCGARVTHNLFHEGPHAAIL
                     FGGREHRIAYNEIHSVCLVAGEMGAIYTGRDWTLVGNVIENNHIHDIYRACPQPTRGI
                     MLDDGVGGLTVRNNLFVRCAEGLCLAAIGNVVSNNMFVACFPAIGTWRNWTAPEEFDL
                     SIRNNGTRLARLAKVPVDASPWKERYPELAMLRDAIRDRKPRPAATYSRIVANVAWKG
                     AGEWIHNHYKTFDGWVIENNLVTEDPLFVSPERDDYRLRTQSPAHKKGIRPLPTERMG
                     LYASSERAVWPVSHPVNTGCGNLTYVPSSDKN"
     gene            complement(17088..18509)
                     /locus_tag="PK576_03940"
     CDS             complement(17088..18509)
                     /locus_tag="PK576_03940"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOE60772.1"
                     /translation="MKKHFDLSLRPVGRHVSAGGCAGLRGGLTLAGLLFAGTLSAWGP
                     HPDITMNAVAAIGPDAPLARALGTETAQLRNTCWIPDVWMQLSGTYYFDDYLFTPERP
                     THPHTSHSHSFLCENGKYGQHSKVLFSHYFRRALQALRTENAQNAARWVGALLHFTED
                     TGAPPHAILEHGPLHGPMENWLNGKQISIAGYVPRVLGNDDASAFEAYWANQERLNNE
                     TIEIANRIKPLVAAGDRAAAEPLILMCANASARNAADLCATLGALLGTDSGGGVLQGR
                     VTSETVPGPLGGVPAKVMLAGTSYSTLADAKGVYRFRNLPPGTYRLLAVRPGSELFEG
                     PVTVSGAETVYDIVMPPARVSGNLLRNPAFALRWTSPDHRDMWYQRGRDRIWRTEAVP
                     VKPGQNCRVHLEWKRPGTEAVLRFTTHEREGKRLAEHALAHPDSRLEAVAPDKATHAH
                     IEVKGCAGLPDAELAYVALTVEK"
     gene            18519..19202
                     /locus_tag="PK576_03945"
     CDS             18519..19202
                     /locus_tag="PK576_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60773.1"
                     /translation="MRISLGAVKPRNRWLRIRPQIALHFLPLLFVKFPFHGFIQIGIG
                     IGIAIGIDCLTTGHFLWFQGFWFQGFMVSRFHPNRDRGRDRYRDRSLNHAHGMALLGF
                     LSTDLHRLHRLFWGIARVFLERQGKFLIFMKFHGFQGFIQIAIGIGIAIGIDYLTTNS
                     RDGTARVFIHRFTQITQIFWGIARVFLERQGKFLIFMMFYVFHGFIQIGIAIGIGIGI
                     DHYKLWPGE"
     gene            complement(19203..20336)
                     /gene="hisC"
                     /locus_tag="PK576_03950"
     CDS             complement(19203..20336)
                     /gene="hisC"
                     /locus_tag="PK576_03950"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOE60774.1"
                     /translation="MAHSCERPAAPAGSVCGSRRLPDARNLIHYPIMYIRQSIAALQA
                     YTPGEQPRDPGIIKLNTNENPYPPSPRVTEALAAFDCSRLRLYPDPLFHALRQRLAEI
                     HGCSDRQIFIGNGSDEILALSTCAFVENHGSIGYFTPSYSLYPVLTEIRNVEKRPVEL
                     TADFRWRMPEAYQASLFLMTNPNAPTSMLFDKPVVTAFCRAFNGVVLVDEAYGDFADT
                     SCMDLALEAGQTNTLVLRTLSKSFSLAGLRFGYAVGPEALIAALYKIKDSYNLDMISQ
                     TLGLAALNDLPHMHENVRRIRATRERLTIELRGRGWRVCDSQTNFLFARPPDGNAKQV
                     FDLLKAAKILVRYFPGPATGEYLRITIGTDDQMDTLLDRLAEM"
     gene            complement(20389..>20944)
                     /locus_tag="PK576_03955"
     CDS             complement(20389..>20944)
                     /locus_tag="PK576_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60775.1"
                     /translation="ANAGWIAFEETGDPRVNLVTGDLSGYVWGANLGWISLGGVRTTV
                     LSAGPDSDGDGIPDPWEMRETGGLTALAGGAHDADGDGVCDVDEYGADTDPLDSQSML
                     AFTAFSRSSTTNRLTWTVEQTRFYELWRSPTLSPHDWSPTGLGVMAPGAGATMTREVD
                     TAPPVGPPPATQFYRVKAVIPLGE"
BASE COUNT         3956 a         6697 c         6253 g         4038 t
ORIGIN      
        1 tccttgccta taaattgcca ggatgccagg attttcagga ttgaacaaca tcatgttaat
       61 catggatata tgcgcccggg cagcggaaaa tcatgtccta taaattgcca ggattacagg
      121 attttcagga ttgaacaacg tcgtgttaat catgccctaa acgtacttcc aaggtttttt
      181 cgcggcagag ccgcgcggcg ggctaactcc taactcctaa ctcctaaatc ctaaataaat
      241 cctaaatccg cgcgtagcgc ggaagacgct cgcgtttccg acagcgcaca cataaaacag
      301 cgctcgactc gcatgccccc gatcacgaac cgattacggg actcacttgt caaggtaaat
      361 gcgactgggg tggttgtatt tactttgaca agtgagctcc ctctttttac ggttatccgg
      421 acgccacgta tgtgctatga tgcgattatg ttacgtgtct ttctgtccgg tgccgcgctc
      481 ttgttgacgt gcctttccgc tttcggcgcg gcggcgcgct ttacggcccc ccggaactat
      541 ccgaacattg ggctgaaact gccggggctc gattcggcgc gggccgatcc cgtgccgatg
      601 ccgcaggcgc aggccttttt gtctgtccgc ggcaccgtgc tgtcgcgcga ggatcgtttc
      661 caaccgtttg aactttggta cggcaaccag tgttgcgcgc aatggcgcga tgcggcgggc
      721 aaccgccttg tcctcggacg cgccacccac cggctgccgg ttttcgagga agagactgtt
      781 ccgcgcgagc gtttcgaaca agcgatgcag gacgggtccg gcagcgaagg gttccgcgac
      841 cgcgcggcgc tcaacgaatg ggtcgcgacc tttgcggata ctccggttta tgatcccgag
      901 cccttgcggt tgaacgcctt ctcgttggac gaggtcctgt tttatccctg tggagaagcg
      961 gacaaactcg tctacgccat ccttccccgc cgtcagggca gtgccgagcg gcccgactgg
     1021 ttctgtgtca cgttccacgc gcccggcgcg tcagacccgg acgcgctgcg cgagcacttc
     1081 gaggaggcgt tcctcagtca aatcagccag ccctcgcgta cggaagtcga tcaggggccg
     1141 caggctgaag aactggatgt ccgctccgcc aaaggtgcgc ggccccctga tctgcccgac
     1201 catcccgtgc gccgcgaggc gcgtaaaagt gtggagaatt acggcgactg gtggttcgcg
     1261 gaaaccgagg gcaccatcat cctgtccgac gcggacaccg atctcggtaa acgtctgatc
     1321 cagacgcttc aggagacgct ccccccgctg tggaaggcgt tcgcgcaact ggtgccgccc
     1381 ctcacgcgtg accgcgacgt cgccctgctg cgccttttcc agcggcgtgc cgactacgcg
     1441 cgttacaccg gtccggcata cgcgtggacc ggcggcatgt ggatgcccgg acggcgtgag
     1501 ctggtgctgc cgctggaggc cgatccccag gtggttctgc gcacggtgcg gcacgagtcc
     1561 tttcaccagt acctctcctt cgcctatggc atggtgccgg ccgcgccttg gttcaatgag
     1621 ggtcacgcct gtctctttga gaatgcccgc atggacgcgg aggggcgcgt gatcctcgac
     1681 gaggacacgg accgtgtctt cgtgctgctg gaccatctcg atacggtcct cacccttttg
     1741 ccacacctgc tgcacgcctc ctacgaagag ttctacgccg gtttcagcgc tggccgccgg
     1801 ttgaagtacg cgatggcgtg gggactggtc tactaccttc agaagggtgc gccgaccgag
     1861 cgcaatacgc ccttcaagca gatcctgccg acgtatgccg acgcgctggc gctcgaactg
     1921 gatggcgcga aagccaccga ggcggccttc gccgatgtgg atatggccgt tttcctcgac
     1981 accttccgcg agttctggct gaaacgccgc ggcacggcca tgcggtatga cccgctgcaa
     2041 cagcgctgag gttttggttt gcgcaacgga ccggccattc gctattatgt tccccattcc
     2101 gattcagcaa cggttttgac gtgacggtgg cccgcttcag cgtggagtcc ggcacggcga
     2161 tccaaggagg acatcatggc ttatcagatc acggacaagt gtgtggcgtg cggtctctgc
     2221 aaagacgcat gcccggtgga ggcgatcagc gaaggcaccc cgatctacac gatcgacgcc
     2281 gacaagtgca ttgattgcgg cgcctgctcg ggtgagtgcc cgaacgaggc gatcatcgcg
     2341 ggttaacccc cggcggtttt atccggatga acaaggcggc ggggttaacc tgccgccttt
     2401 tcctttaacg cctgccgacg ggtacggcct tcggcaaaaa cgtgtcttga aaacgacggg
     2461 aggtaaatgt gaaacaggtt gtcgtactgg ttgccggact gctcgccatc atcggtccgg
     2521 gttgggcgga tgtcgcttgt ccgggcgact atcccgggca tctccaaggc atcgccacgg
     2581 acgaaagcgg gaacctctat tggtcttgga ccacgtttct ggtcaagact gccgcaaccg
     2641 gcacgctttt ggcaaaagtg cccgttccca cgcattacgg cgatctgaca tggcacggcg
     2701 gcaaagtcta tgtggccgtg aatttcgggc agttcaacaa ggagccgggg aaagccaaat
     2761 catgggtctg tgtgcatgac gcggaaacgc tcgccttgct ctcgtcgaac gcggtgcccg
     2821 aggtcgtaca tggcgcgggc ggaatcgagt ggcataacgg gcatttcttt gtggtcggcg
     2881 ggctgcctgc gacacacacg gccaactatg tgtacgagta tacgggatcc ttcgaattcg
     2941 tgaaacgcca tgtgatcgaa agcgggcaga cctttctggg catccagacc gtttgccgca
     3001 tgcgggacgg cacctggtgg ttcggatgtt acggcaagcc tgcggtcgta ttgaaaacag
     3061 acgaccggtt cacgttttgc ggcaaatacc gtttcaacgg cgctgtcggg atggcccgga
     3121 ccccaaagaa cgatgaatta ctgatcgcca caaaccgctt gatcgcaaaa cgcaactcgg
     3181 catcggtccg gcccgtcaaa acggaaacca tcacggcaag aattcaagaa gacaaacact
     3241 gaggggtaga cacgccggaa ggacatcatc ccgcagacct tttgccttga acagccttgg
     3301 ttcacgcatt aaactaattt gtgttgtcca tacgtttcct ttacttatgc gttacgcgat
     3361 cgtttccgac atccacgcca atttcgcagc ctggcggacc gtgctggccg acatcgccga
     3421 cattcgggcg gatcagatca tctgtctcgg cgacatggtc ggctacggtc cgcaacctgt
     3481 ggaagtgctg gaatcggttt accggaccgt gcaggtgacg ttgatgggca accacgacgc
     3541 ggcggtctgc ggacggctgg accccgggct gttcctgccg catgcccgcg ccgccgtgct
     3601 gcgccaccgc gcgcaactct ccgctgcggg aatcgcgtgg ctggaacggc ttccgctggt
     3661 cctgaccgcg cccacgttcc gttgcacgca cggggatttc tccgacccgt ccgcttttta
     3721 ttatatcctc gacgagcagg acgcgtgccc gtcgtggcgg gccacgcagg aaccgctgct
     3781 ctttgcggga cacacccaca tgccgggcat ctatgtcagc ggagcgagcg gacagcccca
     3841 ttttctggaa ccgcgcgact tcacgctcga accggggaaa cggtatatcg tcaatccggg
     3901 cagtgtgggg tatccgcgca cggggccgtt ccgttcctcc tactgcctgt acgacgacga
     3961 ggccggaacc gtcgtattcc gccaactccc gttcgacgat gaggcttacc gcgcggccct
     4021 tcgcgaggcg ggtctggaga acagtgcgcc gtggctggaa cgcctgaccc tgcaccgtta
     4081 ccgtccggca gtgcgcgtgc ggttctcttt cgcgaacccg gttacggcga gccaatcagg
     4141 gagggtgtcc gcgcgccgtt acggtgcgac gggacggcgg cttgcacgga acctcgcgct
     4201 cggcctgctg gcgttggggg cagtgggtgt tgcgggccgc ctcatcttcc gtgccgggca
     4261 cgcgacggcc gaatccgctc ccgtagccat gatgccgccc ttcgagttgc ccgtgcgggc
     4321 cgcctatccc ttgatgcact ccggcgggaa tctgttgccg ccgctgccgg agtctgtcgc
     4381 tccggaccgg cggctcgacg ggtggcgcta cgctctggag gatcgtgacc ggcagcggtt
     4441 cgcccttgga ttgcgtgacg gcggcctcac gctggtggta cgccatgacg gcccccgacg
     4501 gttccagttg gagtctccgc tgatcgatct ggcgggaacc cggatcacgg ctttgcgcct
     4561 aacctcgcgc gtgcgcaaac ccggaccatt cgagggttcc gccacgatcc aactggtcac
     4621 gtacacccgt ttgccgcatg acctgctgca gcggggccgt accgaacaat acgaattgcg
     4681 cactacgcgg cgtcggcttg tgccgcccgg ggccgaacgc aatgtgcgga tccggctcgg
     4741 ccgcgcggtc acgcatgtgc gtttccgcgt ggaggccaca ttcgacgggg tactggaaat
     4801 cgaacaaccg gccctccgcg cggaacagga aggacacttg ccatgattca acgtgtttcc
     4861 gaagaagttt gggcttttga ctgtgaatgg gtgccggatc tgcaggcggg gcgcttgttg
     4921 tatcggttgc cggaggatct gccctctcag gaggttctgc gcgtcatgtg ggaacagggc
     4981 ggcgcaacgc cggagaaccc gaccccgttt ctgaagacga ttctctgccg cgtggtttcg
     5041 gtggccacgg tgatccggcg cgtatcccgc aacggcgaag tacggctctt tctgcaggcc
     5101 ctgcccgaag cgcccgacga cccgtcacaa gacgaggccc acatcctgcg gcgctttctg
     5161 gtggacggca tggccgcgca caatccgcaa ctggtcggct tcaattcgcg gaacgccgac
     5221 ctgcgcatcc tcacgcagcg cgcgctcgtc ctcggtctgc cccaaccggc actctgcgga
     5281 cgtctggcgg caaagccttg ggagagtctc gacgtggacc tcatggatcg gatcagcgga
     5341 tacggcaaaa cctatgcggt ctcgctaaac gagttggcca ccctgtccgg cattccgggc
     5401 aagctggaca cctgcggcga cgacgtatgc ggtctctggc acagcggcca gcggagaaaa
     5461 atcgtggagt acaattgttt cgatgccctc accacctatc tggtgtggct gcgcatggcc
     5521 tatttctcgg ggtgtttcga cgacgagggc taccgcaccg agcagcgccg tttgtacacg
     5581 ctgctccgcg agcgcctgct gaaaccggaa gacgcctacc tgcgagccta cctcgacgca
     5641 tggcgcgacc tcaaacgccg caccggccag ttggacgacg gcgtcttccc gcccgacgat
     5701 cagtgaaccc cgcgtcggct gttctggtaa aacgcgacca gcgtctggat ttccggcagg
     5761 tccgccacct catacttgat gcgttccagc gcctgtgtac ggttgaggcc ttcgcggtag
     5821 atcagcttga acgcctcttt tagcgcggaa cgcgtctcaa ccgagaaacc ccggcgctgc
     5881 aaccccacga tgttcggccc taaaacatgc gtgtccgaac ccacgttttc cgtcagcata
     5941 taaggcggac aatcctgccg gacctttgtc gcgccgccga tcatggcgta ggtcccgatg
     6001 cggcaaaact ggtgaacgcc gaccatgccg ccgatcgtga cgaaatcctc gacagtcact
     6061 tcgcccgcga gggttgtcac attcgccatg atcacatggt tccccaccac acacccgtgt
     6121 gcgatatggc aatacgccat aatcaggcaa tggctcccca cccgcgtcac ttcacccggc
     6181 ttggtcccga gatgcactgt ggaaaactcg cgcagtacgg tttcgtcgcc cacctcgaca
     6241 taggtgacat ccccctcttg gtacttcagg tcctgcgtct tcatgccgac gcaggcgaac
     6301 gggaagacct gacagttctt gcccagcgtg gtatgcccca ccacataggc gtgcgactgc
     6361 accaccgtgc cggccccgat ccggacattc ggtccgatgg tcgcgtaggg gccgacctcc
     6421 acatcggtgt ccagttcggc cttagggtcc acaatcgctg tcggatgaat actcgccata
     6481 cgttcctcct cctccgttgt tttagatgtc acaggttttt ggaaatgagc caggttgcta
     6541 aagtcagaca gacgccgacc ggcagccaca gcagtatgtc cggccgcagt gccgggtatt
     6601 tatccagcga ctgcagcagg atcacgcaaa gataataagc catcgacacc accaagctga
     6661 ccgccatgcc gatcgtggat tccttacgat gggtgcggat gccgagcggc acgccgatca
     6721 tgacgaaaca caacgacgcg aacgcataca cgtaacgcat acggtattcg gtgcggtaga
     6781 tgctcaacag ccgttgcagc gctttgcgcg acaggccttt ttcattggcg cgcaccttgc
     6841 ggatcgcctg ccgcaactcg ttgaaatgga aatctttttc cttgcggaca taacggcgtc
     6901 gtttcatcgc ctcggggatc acatgccgga accggtctgc ggaggccacg ccggggcgat
     6961 caacctcgat cggatcgatc cgcaccttgt acatctccag cacgatgtcc gtcccgttgg
     7021 tggtgaccag cgccttatcc gcccggactt cgcgggtgag cccctcttga ctgtggtcga
     7081 acacgatgag gtcttgaatc cagttgccct ccttacgagc gaaccagagg gtcatcttgg
     7141 ggaagtctgt gatgaaacgg cccgactcca gcagatccag ccccacatcc acactgatca
     7201 ccgagcgcag gttgcggcgc acctcgtggc tgcgcggcac aatctcgttg ttgatgtaca
     7261 gaccgatcag cgtgcagagc gcacccgtca ccagcggcca tctcatgatg gcgaggaggt
     7321 tcaccccgca ggcccgcatg gccgagatct cattatcggc ggaaaggcgg ctgaacacca
     7381 gcaacgcact gacgagcagc gcgagcggca gggtgagccg gagggtttcg ggaaagccca
     7441 cgagcatgaa gagcccgacc gcgcggggcg gcagtccctc gatcatgagc tgcgtgatcc
     7501 gaaccagcag cccgatcgtc atcacaaacg acaggatcag ccaggccagt gcgaacgcac
     7561 ttagaaaggc ctgcaagaca tatcgctcca gaatcttcat gggcgctatt atacaaaaaa
     7621 ggccgcagaa gaaacggctg tttctttgcg gccttccgct ggcaacgttt ccgcggacgg
     7681 cggaagcgtt acgggttcac atacttgacc ttgtccgcgt tggtgcccgc gccgcggatg
     7741 tagatctcca cgcggcggtt ctcgggattc ccgttgacca gatcaggctg gactttgggc
     7801 cggctgtagc cgtatcccac cgcactcaga cgctgcacgg ctacgccctt gcccaccatg
     7861 taggactgca ccgcttgggc ccggcgctcg gagaggtcct gattgtactt ctcgctcgaa
     7921 cgcgtccgcc gatccgcatg gccttcgatc accgccgtgg cctttgtgtt gcgctgcagc
     7981 acgcggacga cttggtcaat ctcactgaag tattggggct tgatcaccgt ggtatcataa
     8041 tcgaactgga tgtagacctc gaacagcatc aggtcatcgg aggggtccag cgggttggtc
     8101 ttgtcctcga tcacctcgtg tccgtccgcc acgccaccct tgtcggtatc acggtccagc
     8161 gggttggtct tgtacttttt gacctcatcc ccgtccgaca acccgtcgaa gtccgtatcg
     8221 ggattgagcg ggtcggtctt gtaggtgcgg acctcctcaa aatcggtcag tccgtcgcca
     8281 tccgtatccg ccttgaaggg gtcggttccc agtttcgcct cctcgtcgtc ggtcaacccg
     8341 tcgccgtccg aatccttggg caggttggtc accgacgagc tgccctcacc gaaactgctg
     8401 ccgccgctgc cgccgaaacg gtaaaccact ccggcatcca acgtgtactg catctcatgg
     8461 cacgtatcaa ccgacatcag tgccctgccg tcagcccgga gggagaggtt gtcggtcaga
     8521 tgatacatgg cgccaatgcc aacccgcggc cccgtcaggg tcgaacgact cccctcctca
     8581 aacacattgc ggtccgccat gtacacggcc caccctgcag aaaggaacgg gtcgaatcgg
     8641 ttataccgat cgaaatgata cagcgcatcc agccccacgc cgtaaatgcg ttgctttttc
     8701 tgcgtccaac gcccgttgtt ggtcatgttg ggcgcgaaag acgcttctgt ctccaccgac
     8761 cagtggtcag aaagatcgta accgagacgc aggttgagat ttaatccctc gtcaatcgcc
     8821 tgatttccct cagggagaat cattccgact ccgggattga cataccagcg gggcgcatcc
     8881 acccgctcct cggccttcac ctgtaaacac aggatcccga cggccgcaac cgtagacaag
     8941 atcacatttg atttgctcat ttgattcctc cttatagccc aaagatgaaa taagtttagc
     9001 ggatacggac gcgcgatgca acaagaatcg gcttacatga cattttttta aggttaggcc
     9061 cataccgctc cgcaaccgga ggcctgaaaa aatacggccc gccacgttcc cccgtggcgg
     9121 gcccgcatcc ttgcggacac aagcttcgca agaaactgcg tgcttctgtg cgcaccccta
     9181 atgtccgaac ctggcgttct gttgcactgg agagggttgg tttccggtgc cgaagcactg
     9241 gacgcgcgca tcgcgtgccc agtccgcttt ttactatgca aaccgcgtgc caacctgcgc
     9301 gtcagaggtt ccacacctgt tcggcgggcg ttttcgctga tgcgaaagcg caggcggtac
     9361 gctctccggc actcagcacg gcccgccctt cttccgaggt caggcgcagg gggccgcatc
     9421 cccggtggcg cagcacgttg gccatcagca gggctttttc cagaccgtcc ccgcgccgga
     9481 agttccacac ctcatccggt tgcgcaagcc gcgcgggccc ctcatagatg gagtctccgg
     9541 gccatgcggc gagcagtgag gccagatcgc tctccgacat ctcggcggca cccgccagac
     9601 tcaccgggtt ccgctccagc gcggccttga caaacggggc atagggcgta cgggtcaggt
     9661 cgcgccaggc gtaaaaggcc agatcggcgg tgaggttgac ctgacgcaga tcctctatcc
     9721 gggcgatcac ctcttcgcgc gtcaggtcca ccgggagcaa gatcggcggg gggggtgtaa
     9781 acgacacgcc cgcggcatcc ggcagccgtg gttccgtgtg gcagaaacgc tgcaactgtt
     9841 cgaccatctc ggccgcgctc atgcaggtgt cgccaatggc ggtgcgcagc gctttcaagt
     9901 cttcggaccg gtccagatca atggcgcgct cctgcacgaa cgcctcgatg tcgttcagca
     9961 cgatcttgcc cggacagcac gaggctgcga acgtctccat cgggatcatg gccagaagtt
    10021 tgtcgcgcgt ggcgtccgtg accttgtacg ggcactcctg ctcaaattga aaagcctgct
    10081 ccagcgacag atagcgatcc actccgtgaa ttggatgccg catcacgaag cactgccggc
    10141 actgcgggtt ctgccgcagg aagttgccca gaatctccgg cgtcagcggc gtcttcaggt
    10201 acgcgcgcaa tccggtggcg aaccgcccgt acgcctcggc gtcgatcgtg gcgcgcgggt
    10261 agagcatgtg gatccatccc gtggggtgcg aaacaatcgt cacccgctcg ttctccagcg
    10321 cgcggcgcgc ctgtttcgaa atctgggtgc cgttgaacca catcgccttc gtcaccagcc
    10381 gccggttgtt gatgagcacg ccgtcgtcca cctccacgaa attctgggag tgcagaggcg
    10441 tggccatcag gaacaccttc tccaatggaa tacccgccac cacgtacagc gccgcggcgt
    10501 agagcgcagc cagcgagaca cactcgcccg ccccggcggc gtacccctca cggaactgaa
    10561 acgcgttcat ggcctcgatc gtctcagtct tccagtaggg gatgatctcc gaggcgtatt
    10621 tgtccaaacc gaaatgggta cggatatcga tatcgaaata gtaggtgtcg ccctgataac
    10681 cgaacagcgc gttggactgc gcgatcacct cgggtgcgag gaagtttgcg aaccgctcaa
    10741 gctcgtggtg ctgatgggtc aacccccatg tctccaaatc gagattgaac acgtagtgat
    10801 ccatgatgta ctgcttcagg cgcagcagcc tgcggtaggg tttcatcttg cggatcccct
    10861 tgaaccacgg atcctcgcgc caccgccaga gacatggcga catgatgttc gccagcacca
    10921 ggctgtacat caactccggg aaaacgaaaa cctccatgtc ggacagcgtg acggccgacg
    10981 aacaccgctc caactcctcc cgggtcattc cgcatcctcc tctctccggc gcgcgcgccg
    11041 gtcccgcacg gcaccgacca cgagcagggt cgcgagcaag accgcgccgg gtatccccaa
    11101 cacccaatct ccccatgccg tgtacggcgc gcgtaacgga acttccggca ccgtcacggg
    11161 cataaccaga aatccgtcga aatccgtgat cgcttcgccg gactccaatc gtttgacgcg
    11221 tcccaccgca tccaccacgc acgacacacc ggagttcgcg gcccgcacca acggcacccc
    11281 gttctccacg gcacggaaca ccgcctgctg ctgatgctgc aacggctcga ccgagccgct
    11341 gaaccacgcg tcgttggtca tcagcaccaa cagccgcgcg ccggcccgca ccgccccgcg
    11401 actcaatgcg gggatcgtgt cctcaaaaca gatcagcggt cccagactca ggatccggcc
    11461 cgcgccccgt gccacatgca gcggccccgg cccgcggccc ggtgtgcaac tcacgccggt
    11521 tggcgcgaaa cgctgtagcc acgggacgag cttgtcgccg gggatatact cgccgaacgg
    11581 caccagatgc cgcttgcggt aacaccccaa tgtctcgccg gtcgcggaaa caagccaggc
    11641 ggcgttatag tagcgacacc cgtcaggcgt cacgcgagaa accccggcac gctcgacctc
    11701 caacgcgccg gtcagaagcg gggcattggc tgctgtcgca ccctcgcgga tcacacgcat
    11761 cgtatcggtg tcgtagggta cggaacccat gactgcggtc tcgggccaga ccaccagatc
    11821 cggcttggcc gcgcccacga gacgggactg gctgaccagc tgttcccact gcatccgaac
    11881 cgtctcgtcg ctgacctcaa aaatgcaggg cgcgttcggc tggatcagcg cgatccggta
    11941 cacgccgctt cgccgtgctt cccggtccca cacgcggatt cgtcccatac cccaaaacca
    12001 tacggcgagc accacgacca gcggcagaaa actttcgatc gaacgcgcaa acctccccgc
    12061 ccaaccgtac gccggaacgg tcgtccgcgg aaccggacgg caccgcgcga cgaacggttc
    12121 cgccatccgc tccaacaggc tcgccaccgc accgttgccc atcacaagca gtgcggacac
    12181 accataaaca cccgtgatcg acgccacctg gatcaacgcc gtgttgtcca cctggctgac
    12241 ccccagaaag ttccaggcga atccggagaa caaccacccc ctcacaacct cggccccgac
    12301 ccacaacaag gggtcgaaca ccgccaccgt tgccacgcgc cgccatcccg gagtctgccc
    12361 cgcccatccc cagacgcggg ccgaggccgc cccggtcatg gccagaaaca gtgcgcacca
    12421 cgccgccagc gctccctgtc ccagcagcac cagaaaccat gggccgttgt tgcggatgat
    12481 cgccggaaac cagcttagtg tcagaagcca gaagagcatc ccgcatagcc aagaccagcc
    12541 gaacgcctca cggggacggc tccgccggat caccagaaac agcggggcca gcgccatcca
    12601 catgccgtga acctgcgcct gcggcggaaa cgccgcccac atcagcagtc cgcttgcaac
    12661 cgccgccgcc cctttgaccc accccttcct cagtcgcgtt atcaccctta atccctacac
    12721 cttcttatac gcgcaccaga caaacgtcac cgtccccact ccccacagca gcgcgatgca
    12781 ataccacggc gaaaggtgcg tcacctgcga cagggtcagc ggtccgaaat cacgccacgc
    12841 ctgcacgctt gttttcagcc aaggctcggc ctcggcatag agcgccgcac ccgcgaacat
    12901 gcccgcgatc acgaacagcg cgtgccaccg accctctgcg accgccccaa ccgccgtacc
    12961 cggacagaat cccgcgaccg cccatcccgc gccgaacagc agccctccga caatcacacc
    13021 cgtattcagc gtccgggggc tcatcgtgat ctgccccgcc tccgccaaca gttgcagccc
    13081 cacgctgccg accagaatcg ccgacagcat gaatttgatg atcgtcatat cgcggaacat
    13141 cagggcgccg acctgtcggt cgaagcgcag caccccggcc ttctgcatca ggaacccgaa
    13201 caaacacccg gtcaccagac ccaaaagcgg ctcactcata cgcgtccctc cgcttgccga
    13261 tcaaccggat gaggaacacc ccgcccgtga aaaaacagat catcccgacc aggccactca
    13321 ccgaaagttg catcgccccg ctgagcccga gcccgctcgg acacccccct gccaaccgtg
    13381 cgccgaacaa ggccgccgct ccgcctagaa acccgcctgc cgcgcgtttc gccacattcg
    13441 gcccgaaacg cttgcgccaa cgctccggaa cccattcgga ttcgaaggtg cggccgctca
    13501 acgccgcgat cagggcacct atggcgatcc cgcacaccaa accggcctgc cagtcccaga
    13561 ccccggggtg cgctttcaga tacgcattcc gggacgcgtg ccccgggagg atccgccgct
    13621 cgatttccgc tgtggcccgc acaatcgtgg tggaggctcc gagatatacg gtgcgtcccg
    13681 tgacccgcgt tgtggcgtaa gccgacgcga ttgcgagcag gccggtcaac gcgcccgtca
    13741 gatagggatt ccagcgtcgc tccctttttt tgctgatcat tccgtctcct tttccgcgtc
    13801 cggactggac ggggattaac gataacacgc cccgaaagac gggtcaatcg cgcacgaatc
    13861 cgctcttgca taatccggca tacccgctta tcctgtgccg cgctttaaag gaatcgcatg
    13921 tactgcacat tgctgaaagc caaactgcac cacctccggg tgacgcaggc gaataaggag
    13981 tacgagggca gcctgacgat cgacgaggat ctgatggacg ccgtcggtat tctgaatttc
    14041 gagaagctgc tgatcgccaa tctcgataac ggcgagcggt tcgaaacgta tgccatccgc
    14101 ggtccgcgcg gcagcggcgt ggcgtgcctc aatggtgcgg cctcgtataa aggccagatc
    14161 ggcgaccgcc tgatcgtgat ggtgtggtgc ctgctggacg acaccgagcg cgcggcgcac
    14221 cacccgaaaa tcgttcgcct tgatgaccgc aaccgcatcg tggccaccgc cctctgaacg
    14281 cgcaccctgc cgaacgcgac gcgattgtgc gggcagggat tcccgcgcat ccggcttgtc
    14341 cgcccttttt tcacctgagc cgtgcccatc cttaccgcca accgattcca aatcttttct
    14401 tctgcgcgat tgacatccgc agtcggggat gattaaatga cccgactttt ccgcatggga
    14461 aaggcccgtg ggggtatagc tcagttggta gagcgcttga atggcattca agaggtcggg
    14521 ggttcgactc cccctacctc caccatatat aaaaaaataa acccgggtgc ttatgccggg
    14581 cagtcctttc cagaacgtca cgtcacattc gcgttttatt ggcccgcgac gagcggcttg
    14641 cggtgtcggg aacgcgggtg tcctcgcccg taacggcgga tcagccggat cggaggcgtc
    14701 cccgttgcag gtcgagtgtc gcgggtcgca agtcaacgta cacgggaacg gggacgtgaa
    14761 cgggcacacc gatcctgata ccggaatccc cggcttgagc ccggcggacg tgttgtgtaa
    14821 gatcggtggt tgtcatgact cgtgttctgt tcccccttca ttcgggtacg gcgcatcacg
    14881 gttgcgcggc tggcggcgga aaggaatgcg gtcatcgggg aggagatcgc atgggtcaaa
    14941 cttgggggcg cgttattccg ctatcgatca gtctgttgct gggcggctgt tccggcatgg
    15001 gcgtggcaca gaaggggacc gtgctgcacg tcgcgccgga cggaaacgat gtctggagcg
    15061 gacggctgga acgggccgca aagggtgacg gccccttggc gacgctggat ggcgcgcgtc
    15121 gggcggtgcg tgccttgcgg acatccggca ccttgccgcc gggaccggtg acggttcttt
    15181 tcgcgccggg cgtttatcgg caaaccgcgc cgcttttttt cacagaacag gacgcgggta
    15241 ccgcggcatc gccggtccgg tatgtggcgc ggcccggcac ggaagtgatt ttttctggcg
    15301 gcatcgtgat ctcgccggac tgggaagtcc gggacgatgc ggcggcggcc cggttggatg
    15361 cggcggcgcg gccccatgtc cggatctatg acattggaaa cggtcgcctg ccggagatcg
    15421 ccgagccggg ccagaagggt tggttcgccc cgctctggat atcggaaaac ggaatcccgc
    15481 tgactgccgc ccgctggccc aatacgggat tcgtgaaaat cgcggacgcc acgcgttcga
    15541 aaaagggatc ggccccttcg ccggaagcga acgggttcac ctatgcgggt gaccgcccgg
    15601 accggtgggt tggggaggct gatccgcgcg tctccggttt tctcggcaac gactgggctg
    15661 cggcgaatgt gcgcgtcgcc tcgatcgatc ccgctctcaa gcggatcacg cagggcaagc
    15721 cttccagtgc gtacggtttc cggaaaggag gccgctggtt cgggtacaat cttttgtgcg
    15781 agttggacgc gccgggggag gtttatgccg atctgaaggc tgaacgcctt tttgtgtggc
    15841 cgaagacgga cacgaaaaat gcggaacgtg aggttgccag atgccgggcg cttctgattc
    15901 tgcgggaaac cgcccatctc acgttcgaag ggatcacctt cacctctgcg ttggagtccg
    15961 gtgttgaagt caggcggagt gaggatctgg gtttcacgcg ctgtcaattc cgctccatcg
    16021 cggggacggg gctgctcatg gagtccgtgc gccgtgtgcg ggtggccgga tgcgagttca
    16081 cccatttggg ggcgggcggt ctctccgcct cctcgggcga cgcccagcaa ttgacgcccg
    16141 gcgggctggt cgtcgataac aatcactttg cgtttttcgg actcacctct ctcagctatg
    16201 cgcccgccct ccagctcagc ggctgcggcg cacgggtgac gcacaacctg tttcatgaag
    16261 gcccccatgc ggcgatcctg ttcgggggac gcgaacaccg catcgcatac aacgaaatcc
    16321 acagcgtctg tctggtggca ggggaaatgg gtgcgatcta taccggacga gactggaccc
    16381 tggtcggcaa cgtgatcgag aacaaccata tccacgatat ctaccgtgct tgtccgcaac
    16441 cgacccgggg tatcatgttg gatgacggtg tgggcggttt gaccgtacgg aacaatcttt
    16501 tcgtgcgttg cgccgaggga ctttgcctgg ccgcgatcgg taacgtggtt tccaacaaca
    16561 tgttcgtcgc gtgttttccg gcgatcggca cctggcggaa ctggaccgct ccggaggagt
    16621 ttgacctctc catccggaac aatggcacgc ggctcgcgcg gttggcgaaa gtgccggtgg
    16681 acgcgtcgcc atggaaggag cgctatccgg aattggccat gcttcgcgac gccatccggg
    16741 atcggaaacc gcgtcccgcc gcgacctaca gccggattgt cgccaacgtg gcttggaagg
    16801 gcgcgggcga gtggatccac aaccactaca agacgtttga cggatgggtc atcgaaaaca
    16861 acttggtaac ggaagatccg ctgtttgtct ctcccgagcg ggacgattac cgtctccgga
    16921 cccaatcgcc cgcacacaaa aaagggatcc gtcctctgcc caccgaaaga atgggactgt
    16981 atgccagctc cgaacgggcg gtgtggccgg tttcgcatcc ggtcaacacc ggatgcggca
    17041 acctgacgta tgttccgtca tccgacaaaa actgaaatgc cgccgtctca cttttccacg
    17101 gtcagcgcca cataggccag ctcggcgtcc ggcagacccg cgcacccttt cacctcgata
    17161 tgtgcgtggg tcgccttgtc cggcgccacg gcttcgagcc gcgagtccgg atgggctaac
    17221 gcatgctctg caagccgttt cccctcgcgc tcatgcgtgg tgaagcgcag caccgcttcg
    17281 gtgccgggac gtttccattc gagatggacg cggcagttct gacccggctt gacgggcacc
    17341 gcctcggtgc gccagatccg gtcccgtccg cgctggtacc acatgtcccg gtggtcgggc
    17401 gaggtccacc gcaaggcgaa cgccggattg cgcaacaggt tcccgctgac ccgcgcgggc
    17461 ggcatcacga tgtcgtacac ggtctccgcg cccgagaccg tgaccggccc ttcgaacaac
    17521 tcgctgcccg gacgcactgc cagcaggcgg taggtgccgg gagggagatt gcggaaccgg
    17581 tatacgccct tagcgtccgc cagggtcgaa tacgaggtgc cggcgagcat cactttggcc
    17641 ggcacgccgc ccaacgggcc cggcacggtc tcgctggtga cgcggccctg cagcacaccg
    17701 ccgccggaat cggtgccgag cagggctcca agcgtggcgc acagatcagc ggcgttccgg
    17761 gccgaagcgt tggcgcacat gaggatcagc ggttcggcgg ccgcgcgatc cccagccgcg
    17821 accaacggtt tgatgcggtt ggcgatttcg atggtttcgt tgttcaaccg ttcctgattc
    17881 gcccagtagg cctcgaaagc ggaagcgtca tcgttcccca gcacgcgggg gacatatcct
    17941 gcaatgctga tctgtttgcc gttcagccag ttttccatcg gcccgtgcag cgggccgtgt
    18001 tcgaggatcg cgtggggtgg cgcaccggta tcctctgtga aatgcagcag cgctcctacc
    18061 cagcgcgcgg cgttctgcgc gttctccgtg cgcagtgcct gtaacgcccg gcggaagtag
    18121 tggctgaaca acaccttgct gtgctggccg tacttaccgt tctcgcaaag aaaggagtgc
    18181 gagtgcgatg tgtgaggatg cgtcggacgc tcaggcgtga acaaataatc gtcaaaataa
    18241 taggttccgc tgagctgcat ccaaacatct ggaatccagc atgtgttgcg gagttgcgcc
    18301 gtttccgttc ccagtgcccg cgccagcggc gcatcaggcc cgatggctgc caccgcattc
    18361 atggtgatgt ccggatgggg gccccacgcg gacagtgtcc ccgcaaacag caatccggcc
    18421 agcgtgagcc ctccccgtaa ccctgcgcaa ccgcctgctg aaacgtgccg cccgaccggg
    18481 cgtaacgaca aatcgaaatg ttttttcatg ttgacagcat gcggatttcg ctcggggctg
    18541 tcaagccgcg taatcgctgg ttacgaatcc ggccacaaat cgcattgcac tttttgcccc
    18601 tgcttttcgt gaagtttccg tttcatggtt tcatccaaat cggtatcggt atcgggatcg
    18661 ctatcggaat cgattgttta accacgggac actttctttg gtttcagggt ttctggtttc
    18721 agggtttcat ggtttcaagg tttcatccaa atcgggatcg gggtcgggat cgctatcggg
    18781 atcgatcact taaccacgct cacgggatgg cactgctcgg gtttttatcc acagatttac
    18841 acagattaca cagacttttt tggggcatcg ctcgggtttt tttggaacgt caggggaaat
    18901 tcctgatttt catgaagttt catgggtttc agggtttcat ccaaatcgct atcgggatcg
    18961 gtatcgctat cgggatcgat tatttaacca cgaactcacg ggatggcact gctcgggttt
    19021 ttatccacag atttacacag attacgcaga ttttttgggg catcgctcgg gtttttttgg
    19081 aacgccaggg gaaattcctg attttcatga tgttttatgt gtttcatggt ttcatccaaa
    19141 tcgggatcgc tatcggtatc ggtatcggta tcgatcatta taaattgtgg ccgggggagt
    19201 aatcacatct cggcgagccg gtcgagcagt gtgtccatct ggtcatccgt accgatggtg
    19261 atccggagat actcgccggt cgcaggtccg gggaaataac gcaccaagat cttcgctgcc
    19321 ttgagcagat caaagacctg tttcgcattg ccgtccggcg gacgcgcgaa gaggaagttg
    19381 gtctgcgaat cacagacgcg ccaaccgcgg ccgcgcaatt caatcgtcag gcgttcgcgc
    19441 gtcgcgcgga ttctccggac attctcgtgc atgtggggca gatcgttcag cgcggccagt
    19501 cccagagtct gggagatcat gtccagattg taggagtctt tgattttgta cagcgcggcg
    19561 atcagcgctt ccggacccac ggcgtacccg aaccggagcc cggctagcga aaaggatttg
    19621 gagagcgtgc gcaagaccag cgtgttggtc tggcccgcct ccagagcgag atccatgcag
    19681 gaggtgtcgg cgaaatcgcc gtacgcctcg tcaaccaaca ccacaccgtt gaacgcgcgg
    19741 cagaatgcgg tcaccaccgg tttgtcgaac agcatgctgg tgggcgcgtt cggattggtc
    19801 atcagaaaca gcgaagcctg gtaagcctcc ggcatgcgcc agcggaaatc cgctgtcagt
    19861 tccaccggac gtttttccac attgcggatc tccgtcagta ccggatagag cgagtacgag
    19921 ggcgtgaagt agccgatgct gccgtggttt tccacaaagg cgcaggtgct gagcgcgagg
    19981 atctcatccg agccgttacc gatgaagatc tggcggtccg aacagccgtg gatctccgcg
    20041 agacgctgac gcaacgcatg gaagagcgga tcagggtaga ggcgcagccg ggaacagtcg
    20101 aacgcggcca gagcctcggt cacacgggga ctgggaggat aggggttctc gttggtgttg
    20161 agtttaatga tccccggatc gcgcggttgc tcaccggggg tataggcctg cagggccgca
    20221 atgctctgac ggatgtacat gatcggatag tgtatcagat tccgtgcgtc cggcaaccgt
    20281 cgcgaaccgc aaacgctccc tgccggtgcg gcgggacgtt cacagctatg cgccatcggt
    20341 gcggagccgg accgccgaaa ccgccgcctc acccagccct tttttaggtt actcccccag
    20401 cgggatcacg gccttgacgc ggtagaactg cgtggcgggc ggcgggccga cgggtggagc
    20461 ggtgtcgacc tcgcgcgtca tggtggcgcc cgcgccggga gccatcacgc ccaagcccgt
    20521 cggcgaccag tcatggggcg acagcgtcgg cgaccgccac agctcgtaga agcgggtctg
    20581 ctcaacagtc cacgtcaggc ggttggtcgt gctggaccgc gagaacgcgg tgaaggccag
    20641 catcgactgg ctgtccagcg ggtcggtgtc ggcaccgtat tcgtcaacgt cgcacacgcc
    20701 gtcgccgtcc gcgtcgtgcg ccccgcccgc cagggcggtg aggccgccgg tctcccgcat
    20761 ctcccacggg tcggggatgc cgtcgccgtc gctgtccggt cccgcgctca gcaccgtcgt
    20821 gcggacgccg ccgaggctga tccagccgag gttcgctccc cacacatagc cgctcaggtc
    20881 gccggttacg agattcaccc gcgggtcgcc cgtctcctcg aaggcgatcc agcccgcgtt
    20941 cgcg
//
LOCUS       DAOSLE010000034        20595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2840_length_20595_cov_8.194888, whole genome shotgun sequence.
ACCESSION   DAOSLE010000034 DAOSLE010000000
VERSION     DAOSLE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2840_length_20595_cov_8.194888"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1418)
                     /locus_tag="PK576_03960"
     CDS             complement(99..1418)
                     /locus_tag="PK576_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60776.1"
                     /translation="MSISARTTKAVLCFSRVLAFGCGVALQQIAAFGEPASLTGSARD
                     EVVSRFRLGPFFEYRATREGGVFWAVRPFYADVRDPVSDTHVTDVAWPLGTFHRDHQQ
                     SWWRLLLAYGSDDDVTRDDAAWKTVILPVWFEGRTRAGEDYWALFPLYGHLPHMALMD
                     DIDFTLFPLYFNYEVNGVERVYTPWPFFSRLTENPHLREEGVFPFWCRQINTKKKTDR
                     RYVLWPFWNSARYEDERNPGSSFLLFPLYGEVNRARESQRLVLPPFFSYARTDSAERW
                     RLPWPFYETREIWETRTTEASFQRSYWPFYGEFEGSDERRRYAAWPLLKYTRTESPKR
                     LTERTRFFPFYTCETIWQPSPGAPGLRETERYTRVWPFFVRETTSRGSRFRALEFNLI
                     RHSGGIERNWVPFWTLYERVELPSGMARHDLLWGLVRWTTGRRGGSE"
     gene            complement(1393..2475)
                     /locus_tag="PK576_03965"
     CDS             complement(1393..2475)
                     /locus_tag="PK576_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOE60777.1"
                     /translation="MIRNMSDITWEAFEPLPFFEKATYLMQENGMPYHTLFAQQFGYE
                     LLDKLATLANEIRSIAKTREGTGFLREQASHKRAMLYFSQPSSRTFLSFVSACQILGV
                     PTGEVRDTSISSEFKGESREDSVRTFSSYFDLIIMRTPEKGLAERMAWALSNSERPIP
                     IINAGSGQDQHPTQALLDIYTLLRSFERSGGLRDRTVVFCGDLLRGRTVRSLSYLLTN
                     YPNIRQIFVAPPSLQVCPDVLAMLKGKDVTFELTDDFRAAIRQADAVYMTRIQDEWDA
                     AKGDSDKIDTRQFKFGLDELKILPQHAILMHPLPRRDEISKSVDSDPRAMYWRQMRNG
                     MWIRAALIAVTFGCEKHIHEYFRQND"
     gene            complement(2524..3528)
                     /gene="argF"
                     /locus_tag="PK576_03970"
     CDS             complement(2524..3528)
                     /gene="argF"
                     /locus_tag="PK576_03970"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOE60778.1"
                     /translation="MLNWMPELYGRSLLRMVDLSDEEMLNVIDLAVALKARRRAGIRG
                     DLLSRRHVALIFEKSSTRTRNSASIAIRDEGGTAEYLTKNDLHFGAKESVKDTARVLG
                     RLFDGILFRGYAQQTVETLAAYAGVPVWNGLTDVFHPTQILADLMTIQENFGRLKGLK
                     VAYVGDGRNNVANSLMFGCAKAGIHYVNCTPSDLLPDPDLVREAGEIARRHGAGVEVV
                     TDPVRGVAGANVLYTDVWVSMGEEAQKEKRLRLLRPYQVNRALVRATGNEDGGLIFLH
                     CLPAFHDHETTVTAKSGALEVTDEVFEAPYSKVFDEAENRMHTIKALYVSALTDLSAL
                     "
     gene            complement(3599..6763)
                     /locus_tag="PK576_03975"
     CDS             complement(3599..6763)
                     /locus_tag="PK576_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60779.1"
                     /translation="MSGTARLIGITMLAACVAAGEGTLAYWPLDTFRSEADLRDIAGL
                     HSLAATGSCAVTPSFRMPLAVLPAYESLPAASRGEGRNRGSVLFSGVPAAELRAAGLG
                     RALVWTNAFTVEGWVRCADDPAEGVSWPLFGAGRPGTGWRVALCRDAQGPAFVLHADA
                     AADRLTLRHRFRDVTKDASYVWRHMCLTYDGTGAGCGVWEFFVNRVSLGVATNPVRAV
                     CADLPGGFFLGGAGEADDAFYGQMDLWRVSAGVLPPAAWLCAAVPRTLAYWPMDRLPN
                     GTPDLRDLTGGPYALAPGRDGGISVAPEQVSGRLPTEPGLWRRSRPARANTGSVRLDG
                     ALGQRSLLVAPDLGLRCDLTNRFTVEGWFRKSANPTERFWQVVGARDNSNGWMLSLRP
                     DAAAVRFHLHVSDVAQGGRLQFERSFVNADVTACTNWCHLALTYDPLRNGVGVWELFL
                     DGVSQGSVANPAPPDRSHGWRDLLLGGRPSLSNSFSGWLDCWRVSDSVLAPEQFLCHA
                     ADTVAAPRPERYTDVRRLDGSMMLPFQRARGRPFCMVLRDGSWLAVQGVEPVPPRSGF
                     CDVAVSVSRDRGKTWSAPVMPVTVKGGEEVSAIGYQTPFGRIYLFWTDEAVRGYGFLF
                     SDDGGVAWSTERLRLTSAAAGQTGGGMPVGIGVPVSLADGAVCLPLVMRREPDGAIEG
                     RVAFSRNLYSERNAARVRFEVTESTGPGFRAMTDPGEGAFYCLAAPDGKTVCGIAGSA
                     QGPVRCANPDGKGNGMPLEKLRFESGQTLAAVTGHVSAAVLSGDRMLLLWRGRSGERC
                     GRGGSVWGSGARQIGDPKTRWSEPELLLYGDGAGGEVCLGSVWEQDNQCWLAVRQGRV
                     VRAYPLDRLRTDGLWPDPMARSVSTNGLVFAYGGTGPTVVPAAFGAHGRPGWSVALWC
                     ALADAEPGAVLFSTWNGRRGLRVSMARSRGAPSLRLEAYDGNRRAVCTTAPGALTAEK
                     THQVAFVCDAACGRMWALAEGRFPEGGTDGGEGTAELPEGFGAVDVPGPATIAGRVSG
                     LQLYDRPLRTAEVVGLARTGPIAP"
     gene            complement(6834..7457)
                     /locus_tag="PK576_03980"
     CDS             complement(6834..7457)
                     /locus_tag="PK576_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOE60780.1"
                     /translation="MSIEPIREALALGKAAEVTQSVTLALEQGVDASVILNDGLCQAM
                     GALGERFRKGEVFVPQVLLAARAMQKGLEVLKPHLVAAGVEPVGTAVIGTIRGDLHDI
                     GKNLVGMMLEGAGFRVVDAGVGVTAATFVELARTHGARIIGVSALLTTTMVHMREVVE
                     AARSSGLDVRVIIGGAPVTQAFCDEIGADGYSPDAASAAQLALRLIR"
     gene            complement(7494..9158)
                     /locus_tag="PK576_03985"
     CDS             complement(7494..9158)
                     /locus_tag="PK576_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60781.1"
                     /translation="MADTQNGKEDTRQWFLRINGETVFGPVSTEGLIVWAEQGRILPG
                     HEISTDRKKWVQAVSEPLLDMCWFVDDGMGELRGPLNRCAAEALLKSGKVPEGSVLVS
                     TEELEADASADLSSAPRPGPLRSAAPDAEVLERNRELEAQVAELRERLARLSESDALE
                     TLRRECGTLAGQVQEVTEQRDSLLRNAEKDARANERKIEGLKQQIRKLEQRCEEADGR
                     LLLSDAAAKQAQALQRAEAETQAKCAAAEKELVTVREELAALSVTCERLRGATEECET
                     LRTRLAEAEAQASEAKERLTVLKGERETLEAALREQEVAFAELLTDANNRDNAYQEQI
                     ATLTQACALSPEETTRFYEDQAALFELMKSEADALAERLESERTHLEQLKTWSTQRQQ
                     FLSELRQTLLQRLGVTPADMTRRALRGQPSDPNANRLRTEFDNMKVAYEREVRLATER
                     ERDLQRKIRVMEAEAGKWRERAAVGDKLGRRLQEMEEVLQNREQELADERKSREAERE
                     QYQGNQQALLLRIEALEKASRPVTPDDVQAAEARAVKLASWMRLKR"
     gene            complement(9226..10422)
                     /locus_tag="PK576_03990"
     CDS             complement(9226..10422)
                     /locus_tag="PK576_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60782.1"
                     /translation="MGKRKGGEKAVWRVRNEQGEQFGPVDFQTLQTWAADGRLAPTSE
                     ISENGTVWQRVTTRAELGMDWVAEVTPGAFYGPIHGQAMRDLVQEGSLSGETAFFTRQ
                     PLDAADRARLEEEARAALTRERDAERERARGLEEQAARLRAERKAWEIEGESLRQRLT
                     QAGEEVKALRDREAAAVAAAADQAQRFEEERGQLREAVKCAERSLAEQATRIASLEGE
                     LADARREADQNENVHRRVQAMEEEAAGLRREVTEAAAQLSERQSRLDALQSETDALRS
                     EAADTRRRVEAARALARQAAEALDSVPSEGDAVRLEAEPVDEPAVRKTEAGTRNSREV
                     PRETQVIEPEVLAPEQKRPSHGKPPVIRVASQAKPGLSLAELERQARRELERLGERGK
                     SFFKKK"
     gene            10603..11364
                     /locus_tag="PK576_03995"
     CDS             10603..11364
                     /locus_tag="PK576_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60783.1"
                     /translation="MNACSSTPFLKRPTAWMAAPLLWIAGVVVLSVIGLTATPDWIAN
                     HFDQDGASPVEAATIALFWLQIAFFWLVPPMRPGRWRAFWLTNFSLLTFFAICRQLDW
                     HRLLVTPSGLPGTTRGTPFKMKFLLNARNPLPDRILVALCFVLVIALCGGTLLYFLPR
                     LLRGLFRMHPVCWTIGFFGGTLILIQIFDRMSSVLRKDFGIHLTDRQHALTTALEEGL
                     ELLLPLFIILAVLQAHFIYLDSDAESGPLARHRAL"
     gene            11402..12091
                     /locus_tag="PK576_04000"
     CDS             11402..12091
                     /locus_tag="PK576_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE60784.1"
                     /translation="MRILVIEDDDKIQSFIGRGLSQEGHTVDMASTGTEGLRLWQCTQ
                     YDAVVLDIMLPERNGIDLLRQMRHKGDPTPVLVLSAKSAVEDRVTGLEIGADDYMTKP
                     FSFSELIARLHAITRRMQSPAQSGKDATSLSLGGVTLDLLRRTVVRENRKIELQPREF
                     ALLELLMRNANRPLNKTVILERIWDYSFDPQTNVVDVLVCRLRTKLDAGFSVKRIQTM
                     RGIGYVFRTTE"
     gene            12069..13499
                     /locus_tag="PK576_04005"
     CDS             12069..13499
                     /locus_tag="PK576_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE60785.1"
                     /translation="MFSARRNNRLRSFAMYLGMGYLIVYALCTGVIFFLITRVITVSA
                     RGFDREDVLAESEELVELLTQNTDGNLLAEQVTMGRYPPSTIFIVRVINRRGLVEYTI
                     TWPKKIDLPDWRAFAEGDENLPREGLSEYYIRPLKRHVQIQTTHLPDGRVLQVGKGSF
                     LEVDQKSMLVRILLIYAALSTFFSIASGIFMMLITLRPIHRITKSMSDIIRSQAFETG
                     APPVRSLIAELDTLGQLFTIMTGKYANLIRAMRETMENVAHDFRTPLARIRGASELAL
                     HREHLPAELTETLADIIEDCDRLQLQLQNLMDAREMESGFVRLNRQPFRLNQLLGEIV
                     DLYSLLAEEKRLTLSYVNACDRDVLFDGDRNRLARAFANLMDNALKYTPNGGRVDLTL
                     ETGPDAVRVCVRDTGIGISEAEQTLVWQRLFRGKQARETEKGLGLGLNIVQIIISAHG
                     GTVTLESRPGAGSVFRVTLPFRDAPS"
     gene            complement(13562..14296)
                     /locus_tag="PK576_04010"
     CDS             complement(13562..14296)
                     /locus_tag="PK576_04010"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOE60786.1"
                     /translation="MTGKGRQLAGLLAEWLYPRVCPNSSCGKPSDRPGRSLCWECFRT
                     VELYRESLCERCGAHAEGRVGHAFECGVCREVRPHFDRARSAGRFRGTLREMIHGFKY
                     GKALWLCQDLTDFLAGCLTAHFDPVAVDVVVPVPLHPVRERERSYNQAALLARALAER
                     IGRRYDDRALRRMRHTETQTHLDAVRRRLNMRGAFEVVRPAWVTQRCVLLVDDVMTTG
                     ATLDACAHALKRAGARTVWALTVARG"
     gene            complement(14310..>15146)
                     /locus_tag="PK576_04015"
     CDS             complement(14310..>15146)
                     /locus_tag="PK576_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOE60787.1"
                     /translation="PVGPSGETIMDYSVFDAVRAGFNRVVFVIRRDFEKAFRETIGAK
                     YAGAVEVDYAFQALDDLPAGFTVPAGRTKPWGTAHAIRSARQVVGAPFAAINADDFYG
                     QDAFARLGAFLSASRPAPKPRFAMVGYRLDQTLSEHGGVARGICRVTPEGMLAGVTEM
                     TRLTRVADGVENQEDPARPVRLNGTERVSMNLWGFTPALFAALEERFPAWLAAHAADP
                     KAEWYIPFVVDGMIREGAAEVEVLPTESAWFGVTYREDRPAVMAAVQARVRDGVYPDK
                     LW"
     assembly_gap    15147..15246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15247..15647)
                     /locus_tag="PK576_04020"
     CDS             complement(<15247..15647)
                     /locus_tag="PK576_04020"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOE60788.1"
                     /translation="MRLLVMDVGNTSTGIGLYDRGVISRVTHVRGGIVKRPAACAAAV
                     RKAIRGGIHGAVLGSVVPRANAAWRRLVRNETGCAPLVVRAGIPMEVTIDYPAPETIG
                     ADRLAGACGGVRRYGAPLIVADFGTALTFDVV"
     gene            complement(15651..16517)
                     /gene="nadC"
                     /locus_tag="PK576_04025"
     CDS             complement(15651..16517)
                     /gene="nadC"
                     /locus_tag="PK576_04025"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOE60789.1"
                     /translation="MEGDGVMGDPRAVAVVRAALEEDIDTGDATTRALVDPQIEATAV
                     LLTREPCRVAGVGVGQTAFLCVDASLRIETLRPDGHAAAAGDVLMTLRGRAASILTAE
                     RTALNFMQRLCGIATLTARFVEAVKPYGTLILDTRKTTPNLRLFEKYAVTCGGGVNHR
                     FGLYDRVLIKDNHRRLWLGGDPDRLDLAVAAARKAYPALAVEVEVESEAECVSALRGK
                     PEWIMLDNMDCGLMRTCVALCRGKAKTEASGGITLDRAREVAATGVDAISLGCLTHSA
                     PAVDLSLEWSWD"
     gene            complement(16507..17286)
                     /locus_tag="PK576_04030"
     CDS             complement(16507..17286)
                     /locus_tag="PK576_04030"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE60790.1"
                     /translation="MRRGVWRLRGFARSAPAAIGLVAVCLGVFLIQQTAARVDFVAVY
                     GYRYGYDQALLHCFGLSWPLLREGFVWQLVTYMFLHASWQHLAMNMLTVLLLGSGLEA
                     EIGGRRFKWIFLTGGLLGGLGWLMVTALTPLLPSAEALTQWLPRTAAAWLQAGASSGQ
                     TLDVALCIGASGGVFALIGAYSALFPRREVYVLLLFFPLRLKARTLAWFLGAVTVAEA
                     VFVQSRVAYAAHLAGGIAGYLCGCALRTEAGDWRGGVPYGG"
     gene            complement(17318..17542)
                     /locus_tag="PK576_04035"
     CDS             complement(17318..17542)
                     /locus_tag="PK576_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60791.1"
                     /translation="MKLKKSSIEAVEETKPATGGAFIAARHRNPVAEVAARQAAGQEM
                     FGSVCAILATVVLLIITALLYVNWNAIKSA"
     gene            complement(17651..19843)
                     /locus_tag="PK576_04040"
     CDS             complement(17651..19843)
                     /locus_tag="PK576_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60792.1"
                     /translation="MAWVSRGVGLCLASAVFGQPPARVLKAVPAWRVAEAPCRLVVEK
                     GPGDFALFRVPAQLDGKPVAAVRAFAATNEVAVRVVWSDASEVTLLASVGALRQKQLI
                     RIYPVPGGQAVAPVAAGLTDPAPLTGVARRTAGMDFPASLADLRMLETRCDGKSVFFA
                     VSDFSKLGTTFKEWFRGDWTRKSHLVELQTWLRVPEDGRYQFGLAGVAPAWLSVDGEE
                     IMAHPAGQPFDAWTVGEDVNLRAGLRRVRIRTVCRQEIDTGLAWKRAGETGIATNVVM
                     VTGGDLCDGRWEWRNRALHPYATAQIGRSYRFRGTDEVFVPYTLRDATACWGTNYVVR
                     WQAGTMPEQEGAAAGTLPVILRKSALPAPLTLRAVSACGEKAEYTTVLQDDGRVWAEY
                     GVTTRITDVPAVCYDDDRVRPIIRIRSSALDGMAYELVSEIERLGGEREETTYTLVTE
                     RGWARVYLTTFRVGEVARVIWSLRHAGVEIGRGCLRFLREPVAVLPDVVSGGLLKVGP
                     DFVVLVASRTARDTEPLSVPQIGTNGVAVLDGFLYGGTQNVECRTLNAEPGNEKPEIR
                     FRVVDMQAVEQSETATGMTLLQPFTVLRSVLPADTVVVAPSLLAISREGGTEGFERRL
                     AAMTGLLSGPACGSPRVLLTVPPAFDVLPGCGCVPGGKPCEHAAAARSYAESVMRVAD
                     AYGVETVDLFTAAKTAEGAVPLVCKGELTPEGRALFEMLLEKKGVSLR"
     gene            20274..>20595
                     /locus_tag="PK576_04045"
     CDS             20274..>20595
                     /locus_tag="PK576_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE60793.1"
                     /translation="MNTPLVPSATRTGLSAFRAVSSQTSCQDRSGFTLIELLVVIAIL
                     GILGSALVVSVQSGYKQARQANCKSNLRQFGVALTIYRGEHDNRTPDWLSNLYPEYVD
                     DRAMY"
BASE COUNT         3877 a         7478 c         5651 g         3489 t
ORIGIN      
        1 ccgaaaccct gaaaccccga aacccaaaaa agcgtcccgt ggttaaatga tcgatcccga
       61 tagcgatccc gatagcgatc ccgatttgga tgggcttttt attcggaacc gccgcgcctc
      121 ccggttgtcc agcgcaccag tccccagagc agatcatggc gcgccatacc ggacggcagt
      181 tcgacacgct cgtacagggt ccagaagggt acccagttcc gttcgatacc cccgctgtgg
      241 cggatgaggt tgaactccag agcacggaac cgcgaaccac gggacgtcgt ctcccgcacg
      301 aagaagggcc agacccgcgt gtaccgctcc gtttcgcgca agcccggggc tccgggcgag
      361 ggttgccaga ttgtctcgca ggtgtagaac ggaaagaaac gcgtacgctc cgtaaggcgc
      421 ttcggactct cggtgcgggt atacttcagc aacggccatg ccgcataacg acgccgctca
      481 tccgacccct cgaactcccc gtagaacggc caataggaac gttgaaacga ggcttcggtg
      541 gtacgggttt cccagatttc acgcgtctca taaaagggcc aaggcaggcg ccagcgctca
      601 gccgaatcgg tccgcgcata ggaaaagaac ggcggcagca ccaatcgttg cgattcgcgc
      661 gcgcggttga cctcgccgta gagaggaaaa agcaggaacg aggagccggg gttgcgttcg
      721 tcctcgtatc gcgcggaatt ccagaacggc cacaaaacat accggcggtc ggtcttcttt
      781 ttggtgttga tttgacggca ccagaacgga aagacgccct cttcacgcag atgcgggttt
      841 tcggtaaggc gggagaagaa gggccagggg gtatagacgc gttcgacccc gttgacctcg
      901 taattgaaat agagcggaaa cagggtgaag tcgatgtcat ccatcagcgc catatgcggc
      961 agatggccgt acagggggaa gagtgcccaa tagtcttcgc ccgcccgtgt gcgtccctca
     1021 aaccacacgg gcaggatcac ggtcttccag gccgcgtcgt cccgggtgac gtcgtcatcc
     1081 gacccgtacg ccagcaacaa ccgccaccag gactgctggt ggtcgcgatg aaaggtgccg
     1141 agcggccacg ccacatccgt cacgtgcgtg tccgatacgg gatcacgcac gtccgcgtag
     1201 aaggggcgca cagcccagaa gacgccgcct tcacgcgtgg cgcggtactc gaaaaacggc
     1261 cccagacgaa aacggctgac cacctcgtcc ctcgccgacc cggtcaggga tgcgggctcc
     1321 ccgaatgccg caatctgttg gagggcgaca ccgcaaccga acgcgagcac gcgcgagaaa
     1381 cacaatacgg ctttagtcgt tctggcggaa atactcatga atgtgcttct cgcaaccaaa
     1441 ggtgacagcg atcagcgcgg cccggatcca catcccgttc cgcatctggc gccagtacat
     1501 cgcgcgggga tcgctgtcca ccgatttgga aatctcatcg cgccgcggca acgggtgcat
     1561 cagaatggcg tgctgcggca gtattttcaa ctcgtcgaga ccgaacttga actgccgcgt
     1621 gtcaatcttg tcactgtccc ccttggccgc atcccactcg tcctgaatgc gggtcatgta
     1681 caccgcatcc gcctgccgga tcgcggcgcg aaaatcgtca gtcaactcga acgtcacatc
     1741 cttccctttg agcatggcca acacatcggg acagacctgc aacgatggcg gtgccacaaa
     1801 aatctgacgg atgttgggat aattcgtcag cagataggag agcgaacgca ccgtgcgccc
     1861 gcgcagcagg tcgccgcaga aaaccacggt gcggtcgcgc aacccgcccg accgctcgaa
     1921 actccgcaac agcgtataga tatccaacag cgcctgggta gggtgctgat cctgtcctga
     1981 tccggcattg atgatcggga tcggccgctc ggagttggag agcgcccacg ccatccgttc
     2041 ggccaacccc ttctccgggg tgcgcatgat gatcaggtcg aaatacgagc tgaacgtccg
     2101 taccgaatcc tcgcgcgact cgcctttgaa ctccgaagag atcgaggtgt cgcgcacctc
     2161 gccggtcggc acgccaagaa tctgacacgc cgaaacaaag gagaggaaag tacgcgaact
     2221 cggctgcgaa aagtaaagca tcgcgcgctt gtgcgaggcc tgctcgcgta aaaacccggt
     2281 cccttcacgc gttttggcga tactccggat ctcgttcgcc agcgtggcga gtttgtccaa
     2341 cagttcatac ccgaactgct gggcgaacag cgtgtggtac ggcatgccgt tctcctgcat
     2401 caagtaggtt gccttctcaa agaacggtaa cggctcgaac gcttcccatg tgatgtcgga
     2461 catgtttcgg atcatacaac cccctttcac cggaccgccg caaggcggcg ccctcccgga
     2521 acctcacaat gccgaaagat cagtcagcgc ggaaacatac agggccttga tggtgtgcat
     2581 ccggttctcg gcctcgtcga acaccttgga gtacggggcc tcgaacacct cgtcggtcac
     2641 ctccaacgcc ccgctttttg cggtcaccgt agtctcgtga tcgtggaaag cgggcagaca
     2701 gtgaaggaag atcaacccgc cgtcctcatt tccggtggcc cgcacgagag cccggttcac
     2761 ctgatagggc cgcaacaggc gcagacgttt ttccttctgc gcctcttcac ccatggagac
     2821 ccatacgtcg gtatagagca cattagcgcc ggccacaccc cgaacgggat ccgtgaccac
     2881 ctcgacgccg gccccgtgcc gacgggcgat ctctccggcc tcccgtacca gatccggatc
     2941 gggcagcagg tcggagggtg tgcagttgac gtagtggatg cccgccttgg cgcaaccgaa
     3001 catcagcgaa tttgccacgt tgttacggcc atcgcccaca tacgcgacct tcagcccctt
     3061 cagccgcccg aagttttcct gaatcgtcat gagatccgcc agaatctgcg tcggatggaa
     3121 gacgtctgtc agaccgttcc agaccgggac gcccgcatag gccgccaggg tctcgaccgt
     3181 ttgctgggcg tacccgcgga acaggatgcc gtcaaagagg cgccccagca cacgtgcggt
     3241 atccttgacc gactccttgg caccgaagtg aagatcgttt ttcgtcaggt attcggcggt
     3301 cccgccctcg tcacggatcg cgatcgaggc ggagttgcgg gttcgggtcg aactcttttc
     3361 gaagatcagg gctacatgac gccgactcag caagtcgccg cgtatccccg cccggcggcg
     3421 cgccttgagc gccaccgcga gatcgatcac attcagcatt tcctcatcac tcaaatccac
     3481 catacgcagc agcgagcgcc cgtacagttc cggcatccag ttcaacatat tcgttctcca
     3541 atgggccgct cagtatacgg ctccatgcca acgggtacaa cccgattgcc gcgcccagtc
     3601 aaggggctat cggcccggtc cgcgccagtc cgaccacctc ggcggtccgc aacggccggt
     3661 catagagttg caacccactc actcgcccgg cgatcgtggc cggtccgggc acatcaacag
     3721 ccccaaaacc ttccgggagt tcggccgtcc cttccccgcc atctgtgcca ccctccggga
     3781 agcgcccctc ggccagcgcc cacatccgcc cgcatgccgc atcacacacg aaggcgacct
     3841 gatgcgtctt ctctgcggtc agcgcgccgg gggcggtcgt gcagacggcc ctgcggttcc
     3901 cgtcgtatgc ctccagcctc agcgagggcg caccccgcga tctggccatt gaaacccgca
     3961 gaccgcgacg tccgttccac gtgctgaaca acaccgcgcc gggctccgcg tctgccaacg
     4021 cgcaccacag cgcgacactc caccccggcc ggccgtgcgc accgaatgcg gctggcacga
     4081 ccgtcggccc cgtaccgccg tacgcaaaaa ccagaccgtt tgtgctgacg gaacgcgcca
     4141 tgggatcagg ccataacccg tctgttctca ggcggtccag cggatatgca cgcacgaccc
     4201 tgccttgacg caccgcgagc caacactggt tatcctgttc ccagacggaa ccgaggcaca
     4261 cctccccgcc cgccccgtca ccatacagca acagttccgg ctcggaccag cgcgtcttgg
     4321 gatctccgat ctgccgcgcg cccgagcccc acacactccc gccccgtccg caccgttcac
     4381 cggatctccc ccgccacaac aacagcatgc ggtccccgga aaggaccgcc gcgctgacat
     4441 gcccggtaac ggccgcgagg gtctgtccgg attcgaaacg cagtttttcc agcggcatcc
     4501 cgttcccttt tccatccgga ttggcgcacc gcaccggacc ctgcgcgctc cccgcgatgc
     4561 cgcacacggt tttcccgtcc ggtgccgcaa gacagtagaa cgcgccctcc cccggatccg
     4621 tcatggcgcg aaaccccgga cccgtacttt ccgtaacctc aaagcgcacc cgtgcggcgt
     4681 tacgttccga atacaggttc cgcgaaaagg cgacccgtcc ctcgatcgcc ccgtccggct
     4741 cgcggcgcat gacgagcggc aggcagaccg caccgtccgc cagagaaacg ggcaccccga
     4801 ttcccaccgg cataccccct ccggtctgtc ctgcggcagc ggacgtcaag cgcagacgct
     4861 cggtcgacca agcaaccccg ccgtcatccg aaaagagaaa gccgtatccc cgcaccgcct
     4921 cgtccgtcca aaagagatag atacggccaa acggcgtctg gtatccgatc gcggaaacct
     4981 cctctccgcc tttgaccgtc acaggcataa ccggcgcaga ccatgtcttt ccgcgatcac
     5041 gactcacact gaccgcaaca tcgcagaatc cgctgcgcgg cggcacaggc tccaccccct
     5101 gcaccgccag ccaggatcca tcccgcaaga ccatgcagaa aggcctgccc cgcgcccgct
     5161 ggaacggcaa catcatactc ccgtcgagtc gccgcacgtc cgtgtaccgt tccggccgcg
     5221 gcgcggccac ggtatccgcc gcatgacaga gaaactgctc cggggccaaa acactgtcgg
     5281 acacgcgcca gcagtccagc cagcccgaga acgagttgga gagggaggga cgccccccca
     5341 gcaacagatc acgccacccg tgggaacgat caggcggggc agggttggcc acgcttccct
     5401 gcgacacgcc gtccagaaac aactcccaca ctcccacccc gttccgaagg ggatcgtagg
     5461 tcagagccag atggcaccag ttcgtgcacg ccgtcacatc ggcattcaca aaagaccgtt
     5521 cgaactgcaa ccgtccgccc tgcgccacgt cactcacatg caggtgaaac cgcactgcgg
     5581 cggcgtcagg ccgcaacgag agcatccatc cgttggaatt atcgcgcgcg ccgaccacct
     5641 gccagaagcg ttcggttgga ttcgcggatt tgcggaacca tccctcgacc gtgaagcggt
     5701 tggtgagatc acaccgcagg cccaaatccg gcgcgaccag cagactgcgc tgtccgagcg
     5761 cgccatccag ccgaacgctt ccggtgttcg cgcgcgccgg gcggctcctg cgccacaaac
     5821 cgggctcagt cggcagacgc ccggacacct gctccggcgc aaccgatatg ccgccgtccc
     5881 gacccggcgc gagcgcatac ggcccgccgg tcagatcccg caaatccggc gtcccgttcg
     5941 gcaaccggtc cataggccag taggcgagcg tgcggggcac tgcggcgcac aaccatgcgg
     6001 cgggcggcag cacacccgca ctcaccctcc agagatccat ctgtccgtaa aaggcgtcgt
     6061 ccgcctcacc cgccccgcca aggaaaaacc cgcccggaag atccgcgcac accgcccgta
     6121 cgggattggt ggcaaccccc agagacacac ggttcacgaa aaactcccag accccgcacc
     6181 ccgcacccgt gccgtcatag gtcaggcaca tatgccgcca cacatacgaa gcgtctttcg
     6241 ttacgtcgcg gaaacggtgt cggagcgtca ggcgatccgc cgcggcatcg gcgtgcaaga
     6301 cgaaggccgg cccctgcgca tcgcgacaca gcgccacgcg ccacccggtg ccggggcggc
     6361 ccgcgccgaa aagcggccac gagacgcctt ccgcaggatc gtccgcgcaa cgcacccacc
     6421 cctcaaccgt aaacgcattc gtccacacga gggcacggcc gagtcccgcg gcacgcaact
     6481 ccgccgcagg cacaccggaa aacaacaccg aaccgcggtt gcggccttca ccacgcgaag
     6541 cggcgggcaa cgattcgtac gccggcagca cggcgagcgg catgcggaac gaaggggtta
     6601 ccgcacacga tcccgtagcg gccagggaat gaagcccggc aatatcgcgt aaatccgcct
     6661 cgctgcggaa ggtatccaac ggccagtacg ccagcgtccc ttctccggcc gcaacacatg
     6721 cggccagcat cgtgatcccg atcagccttg ccgtcccgct cattgcgttt ttcccaccgt
     6781 tcacacctgt aagctccaaa agaagagggt gcgggtacag agtccccgcg ttcctaccgg
     6841 atcagccgca acgccaactg cgccgctgac gccgcatccg gcgaataacc gtccgccccg
     6901 atctcatcgc agaacgcctg agtcaccggc gcgccaccga tgatcacccg aacgtccaac
     6961 cccgatgacc gggccgcctc caccacttcg cgcatgtgga ccatcgtggt ggtcagtaag
     7021 gccgacacac cgatgatccg cgccccatgg gtccgcgcga gttccacaaa cgtcgccgcg
     7081 gtgacgccga ccccggcatc caccacacgg aatcccgcgc cctccagcat catccccacc
     7141 aggtttttcc cgatgtcgtg cagatcaccc cggatcgtgc cgatgaccgc cgtgcccacc
     7201 ggctcgacgc ccgccgccac aagatgcggt ttcagcacct ccaacccctt ctgcatggcg
     7261 cgggcggcca acagcacctg cggcacaaag acctccccct tgcggaaccg ctcccccaac
     7321 gcgcccatcg cctgacataa gccgtcgttc agaatcaccg aagcgtccac accctgttcc
     7381 aacgccagcg tcacactttg cgtcacctcg gccgctttgc ccaaagccaa cgcctctcga
     7441 atcggttcga tgctcatccg ctcacgcact ccggcggaaa accctccgcc cgctcatcgt
     7501 ttcaatcgca tccaagaggc cagcttcacg gctcgcgcct ccgctgcctg cacgtcatca
     7561 ggcgttacgg gccgggatgc cttttccagc gcttcgatgc gcaacagcag cgcctgctgg
     7621 ttcccctggt actgctcgcg ctccgcctcc cggctcttcc tctcgtccgc caattcctgc
     7681 tcccggttct gcaacacctc ctccatctcc tgaaggcgtc gcccgagttt gtctcctact
     7741 gccgcccgtt cccgccattt ccccgcttcg gcctccatca cgcgtatctt gcgctgcaaa
     7801 tcccgctcgc gttccgtcgc caaccgcacc tcacgctcat acgcgacctt catgttgtcg
     7861 aactccgtcc gcaaccggtt cgcattcgga tcggaaggtt gcccgcgcaa cgcgcggcgc
     7921 gtcatgtcgg cgggcgtcac acccaaccgt tgcagtagcg tctgcctgag ttcggacaga
     7981 aactgctgcc gctgtgtgct ccatgttttc agttgttcga gatgggtacg ctccgactcc
     8041 agtctctccg ccagcgcgtc ggcctcgctc ttcatgagtt caaagagcgc ggcctgatcc
     8101 tcgtaaaacc gcgtcgtctc ttccggcgac aacgcgcacg cttgggtcag ggtggcaatc
     8161 tgctcctgat aagcgttatc ccggttgttg gcgtccgtga gcaactcggc aaaggcgacc
     8221 tcctgttcgc gcagcgcagc ctcgagcgtc tcccgctccc ctttcagcac ggtcagccgt
     8281 tcctttgcct ccgaggcttg cgcctccgcc tccgcgagcc gcgttcgcag ggtttcgcac
     8341 tcctcagtcg ccccgcgcag acgttcgcat gtgacggaga gcgccgccaa ttcttcccgc
     8401 accgtcacca actccttttc cgccgccgcg cacttcgcct gcgtctccgc ttcggcgcgc
     8461 tgcaaagcct gcgcctgctt ggctgcggcg tcggagagca gcaagcgtcc gtctgcttct
     8521 tcgcagcgtt gctccagctt gcggatctgc tgtttcagcc cctcgatttt ccgctcgttc
     8581 gcacgcgcgt ccttctcggc attccgcaac agcgagtcgc gctgctcggt cacctcctga
     8641 acctgtccgg ccagtgtgcc gcactcgcgc cgcaacgttt ccagcgcatc gctttccgaa
     8701 agtcgggcca gacgttcgcg caattcggcg acctgcgcct ccagttcccg gttgcgctcc
     8761 agcacctccg catccggcgc ggcagaccgc aacggcccgg gacgaggcgc agacgataga
     8821 tccgcagacg cgtcggcctc gagctcctcc gtggagacca gcaccgaccc ttccggcacc
     8881 ttcccgctct tcagcagcgc ctctgccgcg caccggttca gcggcccccg cagttcgccc
     8941 atgccgtcat ccacaaacca gcacatatca agcagcggct ccgaaaccgc ctgaacccac
     9001 ttcttccggt ccgtggagat ctcatgcccc ggtaaaatcc gtccctgttc cgcccagaca
     9061 atcaggcctt ccgtggaaac cggtccgaaa accgtctcgc cgttgatccg caggaaccac
     9121 tgacgcgtat cctctttgcc gttttgcgtg tctgccataa aaactctgcc gtaccgtatc
     9181 cgaaccccga ttcccgccgc ccctcaggcg cacccgcgcc ccgggtcact tttttttgaa
     9241 aaaggattta ccccgctcgc ccagccgctc cagttcccga cgcgcctgcc gttccagttc
     9301 ggccagcgac aagccgggtt tcgcctgcga ggccacccgg atcacagggg gcttgccgtg
     9361 cgaaggacgt ttttgctccg gcgcgagcac ctccggctca atcacctgtg tttcgcgcgg
     9421 cacctctcgg gagttgcgcg ttcccgcctc cgttttgcga acggcaggct catcaaccgg
     9481 ctccgcctcc agccgcacag cgtccccttc cgaaggaacg gaatcgagcg cctccgccgc
     9541 ctgccgcgcc agcgcgcgcg cggcctcgac acgccgacgc gtatcggccg cctcgctccg
     9601 caatgcgtcc gtttcactct gcagcgcgtc cagacggctt tgcctttcag agagttgcgc
     9661 cgctgcttcc gtcacctccc gccgcaggcc agccgcctct tcttccatcg cctgcacgcg
     9721 ccggtgcacg ttctcgttct ggtccgcctc gcgccgcgcg tcagccaact ccccttccag
     9781 cgaggcgatc cgcgtcgcct gttccgccag actgcgttcc gcgcacttca ccgcttcccg
     9841 cagttgaccg cgttcttcct caaaacgttg cgcctgatcc gcagccgccg caaccgccgc
     9901 agcctcccgg tcccgcagtg ccttgacctc ctccccggct tgggtgaggc gctgacgcaa
     9961 cgactctcct tcgatttccc acgcctttct ttccgcccgc aaccgtgcgg cctgctcctc
    10021 cagaccgcgc gcgcgttccc gctccgcatc ccgctcccgg gtcaacgcgg cgcgcgcctc
    10081 ctcctcaaga cgggcccggt ccgccgcatc gagaggctga cgcgtgaaaa aagccgtctc
    10141 acccgacaac gacccctctt ggaccagatc ccgcatggcc tgtccgtgaa tgggtccgta
    10201 aaaggctccc ggcgtaacct ccgccaccca gtccatcccc agttccgccc gggtcgtcac
    10261 gcgctgccac accgtcccgt tttcggagat ctcgctggtg ggtgccaaac ggccgtccgc
    10321 agcccacgtc tgaagcgtct ggaaatccac agggccgaac tgttcgccct gctcgttgcg
    10381 aacacgccag accgcttttt caccgccctt ccttttcccc acgtttgcca cacgctgcct
    10441 ttccgctgaa aaccgctgaa aaaaccgttc cttatgataa aggaagccgg tcggcttcac
    10501 aatccctttt ccgcacggtt ccgcccctcg aaccccatgc ggcccggtcg ccgcaatggt
    10561 ccgggtcttg tccgaaaccg cggcatggtg tatagttctg ttatgaacgc ttgctcttcg
    10621 acaccttttc tgaaacggcc caccgcttgg atggccgcgc cgttgctctg gatcgcgggt
    10681 gttgtcgtgc tttcggtgat cggtttgacc gccacgcccg actggatcgc gaatcacttc
    10741 gatcaggatg gggcctcgcc ggtggaggcc gcgacgatcg cgctgttctg gctccagatc
    10801 gcctttttct ggctggtgcc gccaatgcgg cccggacgct ggcgcgcctt ctggctgacg
    10861 aatttttcgc tgttgacttt cttcgccatc tgccgccagc tcgactggca caggctcctc
    10921 gtcacacctt cagggctccc cggcaccacg cgcggcacac cgtttaagat gaagtttttg
    10981 ctgaacgccc gcaacccgct cccggaccgg attctggtcg cgctctgctt cgtgctggtc
    11041 atcgcgctgt gcggcggcac gctgctctat tttctgccgc ggctgctccg aggcctcttc
    11101 cggatgcacc cggtgtgctg gacgatcgga ttcttcggcg gcacgctgat cctgatccag
    11161 attttcgacc ggatgagttc ggtgctgcgc aaggatttcg gcatccacct tacggatcgc
    11221 cagcatgcgc tgaccaccgc tctggaggag gggctggagc tgctgctgcc gctcttcatt
    11281 atcctcgcgg tgctccaagc gcacttcatc tatctcgatt ctgacgcgga aagcgggccg
    11341 ctggcccgcc accgtgcact gtaaccccct ttcgcgccgc acggcgcgga aagtctgacc
    11401 catgcgaata ctggtgatcg aagacgacga caagatccag tccttcatcg gccggggatt
    11461 gagtcaggaa ggccataccg tggacatggc ctcgaccggt accgaggggc tgcgtctctg
    11521 gcagtgtacg caatatgacg cggtcgtgct cgacatcatg ctgccggaac ggaacggaat
    11581 cgatttgctc cggcaaatgc gccacaaagg cgacccgacc ccggtattgg tgctcagcgc
    11641 caaaagcgcc gtggaggacc gcgtgaccgg tctggaaatc ggtgccgacg attatatgac
    11701 caaacccttt tcgttttcgg agctgatcgc gcgcctgcac gcgatcaccc ggcgcatgca
    11761 gagcccggcg caatccggta aagacgccac ttcgctcagc ctcggcgggg tgactctcga
    11821 tctgttacgg cgcaccgtcg tgcgcgagaa tcgcaagatc gaactgcagc cgcgcgagtt
    11881 cgccctcctt gaactgctga tgcgtaacgc caaccgcccc ctcaacaaga cggtgatcct
    11941 cgaacggatc tgggattaca gctttgaccc gcaaaccaac gttgtggatg ttctggtctg
    12001 ccggctgcgc acgaaactgg atgccgggtt cagcgtgaag cggatccaga ccatgagggg
    12061 gatcggctat gttttccgca cgacggaata accgcctccg ttccttcgcg atgtatctcg
    12121 gcatgggcta cctgatcgtc tatgccctct gtaccggcgt gatctttttc ctgatcaccc
    12181 gggtcatcac cgtctccgcc cgcggctttg accgcgagga tgtcctggcc gaaagcgagg
    12241 aactcgtcga actcctcacc cagaacaccg acggcaacct gctggccgaa caggtgacca
    12301 tgggacggta tccgccctcc accatcttca tcgtgcgcgt gatcaaccgc cgcgggctgg
    12361 ttgaatacac catcacctgg ccgaagaaga tcgaccttcc ggattggcgt gccttcgcgg
    12421 aaggcgacga aaatctgccc cgcgagggat tgagcgaata ctacatccgg cccttgaaac
    12481 ggcatgtcca gatccagacc acccatctcc cggacggccg tgtgcttcag gtgggcaagg
    12541 gttcgtttct ggaggtggac cagaaaagca tgctggtgcg catcctcctg atctatgcgg
    12601 ccctctccac cttcttctcg atcgcctcgg gcatcttcat gatgctgatc accctgcgcc
    12661 ccatccaccg catcacaaaa tcgatgtccg acatcatccg ttcgcaggcc tttgagaccg
    12721 gtgcgccgcc ggtccgaagc ctgatcgcgg aactcgacac gctagggcag cttttcacga
    12781 tcatgaccgg gaaatacgcc aacctcatcc gggccatgcg cgagaccatg gagaacgtgg
    12841 cgcatgactt ccgcacgccg ctcgcgcgta tccgcggcgc atcggagctg gcgctgcacc
    12901 gcgaacacct tcccgccgaa ctgaccgaga cgctcgcgga catcatcgag gattgtgacc
    12961 gcctccagct tcagctccag aacctgatgg acgcgcgcga aatggagagc gggttcgtcc
    13021 ggctcaaccg ccagcccttc cgcctcaacc aactcctcgg cgaaatcgtc gatctctact
    13081 cgttgcttgc ggaggagaaa cgcctgaccc tctcgtacgt caatgcctgc gaccgcgatg
    13141 tgctgttcga cggggaccgg aaccgtctcg cccgcgcctt cgccaacctc atggacaacg
    13201 ccttgaaata taccccgaac ggcggccgcg tggacctcac gctggaaaca ggccccgacg
    13261 ccgtgcgcgt gtgtgtccgc gacacgggca tcggcatctc cgaagccgaa cagaccctcg
    13321 tctggcaacg cctgttccgc ggcaaacagg cgcgtgaaac cgaaaagggg ctggggctcg
    13381 gcctgaacat cgtccagatc atcatttcgg cgcatggcgg cacggtcacc ctggaaagcc
    13441 gacccggcgc gggcagcgtt ttccgcgtga cgttgccgtt ccgcgatgcg ccaagctgaa
    13501 cttctgcgtt gagataagcc agggtttccg acagcttccc gtaaccggca acccaaaatt
    13561 ctcatccccg tgccacggtc agagcccaca ccgtgcgcgc accggccctt tttagtgcat
    13621 gcgcacaggc gtccaacgtg gcaccggtgg tcatgacatc atcgaccagc agcacacagc
    13681 gctgcgtcac ccatgccggc cggaccacct caaacgcgcc ccgcatgttg aggcgccgac
    13741 ggaccgcgtc cagatgcgtc tgcgtctccg tatggcgcat gcgtcgtaac gcgcggtcat
    13801 cgtagcggcg tccgatccgc tccgccaagg cccgcgccag cagcgccgcc tgattgtagg
    13861 agcgctcgcg ctcgcgaacc ggatgcagcg gcaccggcac caccacatcc acggcaacgg
    13921 gatcgaaatg cgctgtcagg catccggcca aaaaatccgt gaggtcctgg cacagccaaa
    13981 gggcctttcc gtatttgaag ccgtggatca tctcccgtaa ggtgccgcgg aagcgtccgg
    14041 ccgagcgcgc gcggtcgaaa tgcggccgca cctcccggca tacgccgcat tcgaacgcat
    14101 ggcccacacg tccttccgca tgggcgccgc acctttcgca caggctctcc cggtaaagct
    14161 cgacggtgcg gaaacactcc cagcacaacg accggccggg tcggtccgac ggcttgccgc
    14221 aggacgagtt ggggcagacc cgcggataga gccactccgc gagcagaccg gcgagttggc
    14281 gccctttccc ggtcatgtgc gcctccgtgt taccagagtt tatcgggata cacgccatcg
    14341 cggacccgtg cctgcaccgc agccatcacc gcaggcctat cctcccgata ggtcaccccg
    14401 aaccaggcgc tttccgtcgg cagcacctcg acctccgccg caccctcgcg gatcatcccg
    14461 tccaccacaa acggaatgta ccattcggct ttcggatccg ccgcatgcgc cgccagccaa
    14521 gcggggaaac gttcttccaa cgccgcgaac aaagcgggcg tgaagcccca caggttcatc
    14581 gacacccgct ccgtaccgtt cagccggacc ggacgcgcgg gatcctcctg attttcgacg
    14641 ccgtccgcca cacgcgtcaa acgcgtcatc tccgtgactc ccgccagcat tccctccggc
    14701 gtcacgcggc agatgccgcg cgccacgccg ccatgttccg agagcgtctg atccaaacga
    14761 taaccgacca tagcgaagcg tggttttggg gcgggccgcg atgcggagag aaacgccccc
    14821 agccgcgcga acgcatcctg accatagaag tcatccgcat tgatcgcggc aaacggcgcc
    14881 ccgaccacct gccgcgccga acggatcgcg tgcgccgtgc cccacggctt ggtccgcccg
    14941 gccggcaccg tgaaccctgc gggcagatcg tccaaagcct gaaacgcata atccacctcg
    15001 accgccccgg cgtatttcgc gccaatggtc tcgcggaagg ccttctcgaa atcccgccgg
    15061 atcacgaaca ccacgcggtt gaatccggcg cgcacggcat cgaacaccga atagtccatg
    15121 atcgtttcgc cggacggccc caccggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15241 nnnnnnacca catcaaaggt cagcgccgtg ccgaaatccg cgacgatcag cggcgcgccg
    15301 taacgccgca cgccgccgca cgcacccgcc agccggtccg cgccgatcgt ttccggagcg
    15361 ggataatcga tcgtcacttc catcggtatc ccggcccgga ccaccagcgg cgcgcaaccc
    15421 gtttcgttgc gtacgagtcg acgccacgcc gcattggccc gcggcacaac ggatccgaga
    15481 accgcaccgt ggatcccgcc gcggatcgcc ttgcgcactg ccgcggcaca cgccgcaggg
    15541 cgcttcacaa tcccaccccg cacatgcgtg acacgcgaga tcacaccccg gtcatacaga
    15601 ccgatgcctg tcgaggtgtt cccgacatcc atgacgagca atcgcacgac tcagtcccaa
    15661 ctccattcca gtgacagatc cacagcaggc gccgaatgcg tcaggcaccc gagcgagatc
    15721 gcgtcgaccc ccgtcgccgc cacctcgcgc gcacgatcca gcgtgatccc gcccgaggcc
    15781 tcggtcttcg ccttgccgcg gcacagggcc acgcatgtgc gcatcaggcc gcaatccatg
    15841 ttatcgagca taatccattc gggttttccc cgcaacgcgc tgacacactc ggcctcactc
    15901 tcgacttcca cctccaccgc gagtgcggga taagctttcc gcgcagccgc caccgccaaa
    15961 tccaaccggt ccggatcgcc ccccagccat agccgccggt ggttgtcttt gatcagcacc
    16021 cggtcgtaca ggccgaagcg gtggttgacg ccgccgccgc aggtaaccgc gtacttttcg
    16081 aacaaccgca ggttgggtgt ggttttacgc gtatcaagga tcagcgtgcc gtacggtttt
    16141 accgcctcga cgaatcgggc ggtcagcgtg gcgatgccgc acagacgctg catgaaattc
    16201 agcgcggtcc gctctgcggt cagaatggag gcggcccggc cgcgtagggt catcagcaca
    16261 tcccctgcgg ccgcggcatg cccgtcgggt cgaagcgtct cgatccgcag cgacgcatcc
    16321 acgcaaagaa aggccgtctg tcccacacca accccggcca cgcggcatgg ctcgcgcgtg
    16381 agcagcacgg cggtcgcctc gatctgcggg tcgaccagcg cccgggtcgt ggcatcaccg
    16441 gtgtcaatat cctcctccag agccgcgcgc accactgcga cagccctcgg gtcacccatc
    16501 acaccgtcac cctccatacg gcacccctcc tctccaatcc cccgcctccg tgcgcagcgc
    16561 gcatccgcag agatacccgg cgatcccgcc cgccaggtgt gcggcgtagg ccacgcggga
    16621 ctgcacgaaa accgcctccg ccacagtgac ggcacctaaa aaccacgcca gggtccgcgc
    16681 cttcagccgc aacgggaaaa agagcaacag cacatatact tcacgccgtg ggaacagggc
    16741 gctgtacgcg ccgatgagcg caaacactcc gcccgaggca ccgatgcaca gggctacgtc
    16801 cagcgtttgc ccggatgacg cgcccgcctg aagccacgcg gccgcggttc gcggcagcca
    16861 ttgcgtcagc gcctcagcag acggcaacag gggggtcagc gccgtcacca tcagccaccc
    16921 gagcccaccc aacaatccgc cggtcaggaa aatccatttg aaacgccgcc cgccgatctc
    16981 ggcttcaagc ccggagccga gcagcaacac cgtcagcata ttcattgcaa ggtgttgcca
    17041 tgaggcgtgc aggaacatgt aggtcacaag ctgccagaca aacccctcgc gcaacagggg
    17101 ccagctcaat ccgaaacagt gcagcaacgc ctgatcgtac ccgtaccggt atccgtatac
    17161 cgcgacaaaa tcaacccgcg ccgctgtctg ctggatgaga aagacaccaa ggcacacggc
    17221 caccaggccg attgccgcag gcgctgatcg cgcgaaaccc ctcagacgcc agactcctct
    17281 gcgcatccga ttgatcaacc cggcctccga tccggcctca ggcggatttg atcgcgttcc
    17341 agttgacgta cagcagcgcg gtgatgatca gcagcacaac ggtcgcgagg atcgcgcaca
    17401 cgctgccgaa catctcctgt ccggctgcct gacgcgcggc aacctccgcc accggattgc
    17461 ggtgacgcgc ggcaatgaac gcaccccccg tcgcgggctt ggtctcttca accgcctcga
    17521 tagacgattt cttcaatttc atggttacga ttctatcaga acgggccccc gttgagtagc
    17581 gcatttttaa agaaccccac aaccctgact tgcatggggg aatgcacaca gacgcgaaaa
    17641 ccggagagcc tcacctcagc gacacccctt ttttctccag cagcatctca aacagcgcgc
    17701 gcccctccgg ggtcaattcc cccttgcaaa ccaacggcac cgcgccctca gccgtttttg
    17761 cggctgtaaa cagatccacg gtctccaccc catacgcatc ggcaaccctc atcacactct
    17821 cagcatagct ccgcgccgcg gccgcatgct cgcagggttt cccgcccggc acgcaaccgc
    17881 acccgggcaa cacgtcaaac gccggcggca cggtcagcag cacgcggggc gaaccgcatg
    17941 ccggccccga aagcagaccg gtcatcgccg cgaggcgtcg ctcgaaccct tccgttccac
    18001 cctcccggct gatggccagc agcgaaggcg cgaccaccac cgtgtccgca ggcaaaaccg
    18061 agcggagcac ggtgaagggc tgtagcaacg tcatccccgt agccgtctca ctttgctcca
    18121 cggcctgcat atccaccacc cggaagcgga tttctggttt ctcgttcccc ggttcggcgt
    18181 tcagcgttcg gcattcgacg ttctgcgttc ccccatagag gaacccgtcc agcacagcca
    18241 ccccgttggt cccgatctgc ggcacggaca gcggttccgt gtcacgggcc gtgcgggagg
    18301 ccaccagcac cacgaaatcc ggtccgactt tcaacaaccc acccgaaacc acatccggca
    18361 acaccgcaac cggttcgcga agaaaacgca ggcacccccg cccgatctca accccggcat
    18421 ggcgcaagga ccagatgacg cgcgccacct cccccacccg gaaagtcgtg aggtacacgc
    18481 gtgcccaacc gcgttcggtg accagcgtgt acgtggtctc ctcgcgctcc cccccgagac
    18541 gctcgatctc cgagaccaac tcgtaggcca tgccgtccaa cgccgaggaa cggatccgga
    18601 tgatcgggcg gacgcggtcg tcgtcataac agaccgccgg cacgtcggtg atccgggtgg
    18661 tcacaccgta ttcagcccag acccgcccgt cgtcttgaag cacggtcgtg tattccgcct
    18721 tctcgccgca tgccgagacg gcccgcaacg tcagcggggc cggcaacgcg cttttgcgta
    18781 aaatcaccgg caaggttcct gcggcggccc cctcctgttc cggcatcgtg cccgcctgcc
    18841 agcggacgac ataattggtt ccccagcagg ccgtggcgtc gcgcaatgtg tagggcacaa
    18901 acacctcgtc tgttcccctg aaccggtagg aacggccgat ttgcgccgtg gcgtacggat
    18961 ggagcgcgcg gttgcgccac tcccagcggc cgtcgcacaa atccccgcct gtgaccatca
    19021 ccacattggt ggcaatgccc gtttcacccg cgcgcttcca agccagaccg gtgtcgatct
    19081 cctgacggca gaccgtgcgg atgcgcacgc gccgcaaccc ggcccggagg ttcacatcct
    19141 ccccgactgt ccaggcatcg aacggctggc ccgccggatg ggccatgatc tcctccccgt
    19201 ccaccgacag ccaggcgggc gccacgccag ccagcccgaa ctgataacgc ccgtcctccg
    19261 gcacccgcaa ccaagtctgt aattcgacca gatgactttt acgcgtccag tcgccccgga
    19321 accactcttt aaacgtcgtt ccgagcttgc tgaaatcgct gaccgcaaaa aacacggact
    19381 tgccgtcgca gcgcgtctcc agcatccgca gatccgccaa cgacgcgggg aaatccatcc
    19441 ctgccgtcct gcgcgcgacc ccggtcaacg gcgcgggatc ggtcaaaccg gccgcaaccg
    19501 gcgcgactgc ctgcccgccg ggcaccggat agatgcggat cagctgtttc tggcgcaacg
    19561 cgcccacgct cgccagcaat gtcacctcgc tggcatcact ccagaccacc cgcaccgcca
    19621 cctcgttggt cgccgcaaac gcgcgcactg ccgccaccgg cttcccgtcc agttgcgcgg
    19681 gaacccgaaa cagagcgaaa tcgcccggcc ccttctccac caccagacgg cacggcgcct
    19741 ccgccacacg ccacgccggc accgccttca gcacgcgggc cggcggctgc ccgaataccg
    19801 ccgacgccaa gcagaggccg acaccgcggg agacccatgc caaacaccta acgcgggcgg
    19861 agcccgtaac ccaaggtgac ggggaacggg agacgggaac gccgaatgtc gaacgtcgaa
    19921 cgctgaacac cgaagcaagt ggtcgggacg catccccgag gcgtccgcag ctcacggcat
    19981 ggacctgcgt cggtttgcgg ggaaaccgcc ctaccatgct tcgcaccctg gcagggacgc
    20041 atccccgagg cgtccgttcc tcaaacgttg agcgttccct gcgttgccca caacgcagcg
    20101 acgcccctat cccaacgaca ctcctgcata cttcagtatc catacgtttc aaccttgatc
    20161 gtccaaatcc gtgatgccac acgcgctttc ggtccaaaat cccgcgcttc ctccttgctc
    20221 tgctcacccc gtctttggca tgatatttgc gattaactag aggaatgacc ggagtgaaca
    20281 caccattggt tcctagcgca acccgaacgg gcctgtctgc tttccgtgct gtttcttcgc
    20341 agacatcatg ccaagaccgc tccggtttca ccctgatcga gcttctggtg gtgatcgcca
    20401 tcctcggcat tctgggctcc gcgctggtcg tctccgtgca gtccggttac aagcaggcgc
    20461 gccaggccaa ctgcaagtcc aacctgcggc agttcggcgt ggccctgacc atctaccgcg
    20521 gcgagcacga caaccgcacg ccggactggc tctccaacct ctaccccgag tatgtcgacg
    20581 accgcgccat gtacg
//
LOCUS       DAOSLE010000035        20590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2842_length_20590_cov_7.182761, whole genome shotgun sequence.
ACCESSION   DAOSLE010000035 DAOSLE010000000
VERSION     DAOSLE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20590
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_20590_cov_7.182761"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PK576_04050"
     CDS             <1..658
                     /locus_tag="PK576_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60794.1"
                     /translation="IVTDGSGRVLGWEALNGGTPLVPAVTDAAAWALPVLEAGGVAFS
                     AAGGPASPLAFASNAVATVFLAVRPAHSPTNFAALLEVPGASVTAVPRAAQPAYDPED
                     AAGLSVRVNGELSLAFPAAPHIVEVDFPEPVSGGELRIGGGSACAAWRQQWRGNVAGV
                     IAFGAPPSESVRQVVRSYLARRHGIAGAFPPPGGGAVLQAMAQGVDLHGLFSTRLLLW
                     "
     gene            986..1510
                     /locus_tag="PK576_04055"
     CDS             986..1510
                     /locus_tag="PK576_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60795.1"
                     /translation="MECQSYRQGCCGCCVNMRWGDAKIAAFLEANTRAAEQLFPPHGA
                     RPRYRDLVRAHLRCGGWRDHLLMTLLAPLTLGLAAWYWKRRMGSCCFAGFLDRASGRV
                     GCLIHPARAGTPDLRRHAFPLIPTLGCDRALRCPMLDAENPDPDLDWLTVSRRGAASL
                     DGAHPPFPDRVIGQ"
     gene            1548..1697
                     /locus_tag="PK576_04060"
     CDS             1548..1697
                     /locus_tag="PK576_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60796.1"
                     /translation="MANVHVLQIGIGIAIGFGIDCCAHAVARSDKCNDGGDSDPDPDG
                     AAMEP"
     gene            2036..2962
                     /locus_tag="PK576_04065"
     CDS             2036..2962
                     /locus_tag="PK576_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE60797.1"
                     /translation="MMCNAMNHNTVFRTARSSRSPRLCAFALAHLALLLPLGVSADTY
                     GDYTYTVSDGGATITKYNGSGGAVAIPSTFGSHPVTAIGNYAFSEHTLITSVTIPDSV
                     TNICDWAFLLCSSLATVTIGNSVTNIGGYAFAECPSLTAMTIPNSVSDIGTHAFAECP
                     SLTAVTIGNSVTNIGGYAFADCPSLTNILFTGDAPALGYSAFHNNNPATFYYLPGIGD
                     WPSWFGGRPTVCWNPAFSSASSASGAFAVTLTGNPSAALPVRIEASDSLSSPSWLPLS
                     GTTTIPAGGTLNFTDPDASPPPARFYRITFPR"
     gene            3541..4773
                     /locus_tag="PK576_04070"
     CDS             3541..4773
                     /locus_tag="PK576_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60798.1"
                     /translation="MSFEMSRKGFLGMGGSFFAANVFSATAQKDKDGKVIAGFDETDA
                     GKIAQVKWEPFSDKKVRVGIAGHGICKFGCSFGYQNHPNVEIVACTDLDPARCADMQK
                     QLKAPRTFKSCEEMIKEMRGKMDAVYIATDPPSHAPLAILAMENGMHAVSAVPAFWGD
                     IQEEYAQKLVDTVKKTGMIYQMNETTAFRPQCFAMRALYKGGALGEIIYTEGEYWHHG
                     SRPMGSYKGWRNFAAPQWYCTHGNGFYNCVTHGHFTKCACLGVPTHYDAKKSFIGKPG
                     NYGNIYGTEVALLKTSEGGMARMGRGGDTPSAIQETGRCLGTRGQYDEGYKGEATKEE
                     LAKIAYNKPPLPPGVPAGGHGGSHGYLTDDFIRAILLGQKPVVDVICAVNMTIAGIYM
                     HRSAQKDGEWLRIPHFAI"
     gene            4904..7714
                     /gene="ppdK"
                     /locus_tag="PK576_04075"
     CDS             4904..7714
                     /gene="ppdK"
                     /locus_tag="PK576_04075"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOE60799.1"
                     /translation="MKTSRARKSAGKTAKFVYSFGSGRSEGSVSMKTLLGGKGANLAD
                     MSGLGLPVPPGFTITTEACAKYYEVGEKALHALLRDQVTEALRKLEKATGKTFGHGPN
                     PLLVSVRSGAAASMPGMMDTVLNLGLNPKTVEAMIERTGNPRFVWDSYRRFIQMFGNV
                     VMGVEHHEFERELENVKHARGYTLDTELMTEDLQDVVARYMKRVREIKGCAFPTDAME
                     QLWRGIDAVFGSWNNPRAIKYRALNDIRGLLGTAVNVQTMVFGNSGSNSATGVAFTRD
                     PSTGEHKYFYGEYLINAQGEDVVAGIRTPQQITIRGSREWAKARNITEAVRKVQYPAL
                     EEAMPKVFKQLDAIRVKLERHYRDMQDLEFTIEDGKLFILQTRSGKRTAAAAVKIATD
                     LVREKIIDEKTAVLRVEPGQLDQLLHPVFVRSAEAKAKKITVGLPASPGAATGQIVFN
                     AATAELWAREGKKVILCRIETSPEDIGGMNVAAGILTARGGMTSHAAVVARGMGKCCV
                     SGCGELHISYDKKTISCNKLVLKEGDWISLNGSTGLVYEGQIETEDPKLTGPFAMIMA
                     LADKYRKLGVRTNADTPRDTAKAVAFGAEGVGLCRTEHMFFEGDRVISFRRMILVAET
                     VKSLRQALAECTDPEERTRLEQELAAPLKQYRQALKELLPLQRKDFISMFKALKGRPC
                     TVRLLDPPLHEFLPHDEAGQAEMAREMDLEVIQIREVVESLHETNPMLGHRGCRLGIS
                     YPEVTEMQARAICEAAAAVKGSQPEIMVPLVGNVKELTAQKELIHKVLADVERTRKVK
                     LPIRIGTMIEVPRAAVTADQIAAEADFFSFGTNDLTQMTCAFSRDDAGKFLGEYVARG
                     FYDYDPFQVLDQEGVGRMVEIAMQYGRGVKPNLKVGVCGEHGGDPKTVEFCHKLGFSY
                     VSCSPFRVPIARLAAAQAALRS"
     gene            complement(7800..9107)
                     /locus_tag="PK576_04080"
     CDS             complement(7800..9107)
                     /locus_tag="PK576_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60800.1"
                     /translation="MTGTKNKMIGLAVCAVMAGGALCAVRAQTESRPRVLIIGDSISL
                     GYTPFVKAALSNRMEIVHAPGNNQATATGLAHLETWLGTGKWDVIHFNWGLHDLKYID
                     DQGKATDVAKGRQWVPVEQYETNLGTLVRRLKTTGARLIWCSTTPVPEGTAIRIAGSE
                     VAYNEAALRVMRGAGVPVNDLHAFVGPPERRLAMGGRPRDVHYTAAGSKALAGEVVRA
                     IETALESRAGQDGWVELFDGKSLAGWRPYGKPVGTAPGEGWSVEGGLLRKKPKIRGGD
                     LITEKTYGDFELEWEWRISPGGNNGVKYCVTEARPGAPGYEYQMIDDLSAKWVKLPAN
                     ARTASFYAVLPPAADKPLKPAGEWNVSRIVVRGDRCEHSLNGRKVLEYVFGSAEVKAG
                     VAASKFAEVPDFGDKIKGHIMLTDHTDETWFRSIRIREPAAQP"
     gene            9417..10388
                     /locus_tag="PK576_04085"
     CDS             9417..10388
                     /locus_tag="PK576_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE60801.1"
                     /translation="MIRKSLITGGAGFIGSHLARRLLAAGHEVTVIDNLFTGTKANIY
                     DLLDNRRFTFILHDVTQPFWGQFDEVYNLACPASPIHYQRNPVETLKTSFLGIMNVLE
                     LALKTKARVFHTSTSEIYGDPEIHPQPESYWGNVNSIGARSCYDEGKRAAETLCADYV
                     HEYGVDVRMIRIFNTYGPNMHPQDGRVISNFIMQALRNQDITLYGDGSQTRSFQYVDD
                     LIDAILRFMALDHKKVKTFFEGRAMRVPVINIGNPGEYTIRQLAEETLRLLPKSRSRL
                     AFHPLPKDDPRRRKPDITLAGELLGWAPSVALPEGLAKTIAYFQNLV"
     gene            10411..11667
                     /locus_tag="PK576_04090"
     CDS             10411..11667
                     /locus_tag="PK576_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60802.1"
                     /translation="MPFPQAVLPLLNLVCGLALGAALSVAAQTEQPPPPSWGEIGRVN
                     MRGGLRIFWNVGGKDHKANEREALARGFEPVDLLNTYSDYPGKQREKIIPNGKNPWAK
                     PPQFERIIRRNIAMKQGRGLFVHDIEFDFDEEPEALWTNPVLRAASGADTVEAFRKAY
                     YREWGTWYTLPCRWAKEAFPHLPVGIYGPQPFRRDYWGICGKDAQQIDGTHKTDAELW
                     QHIDPAVDFCIASLYCFYDRPDSVFYMGANVEENAQRMALTGDKPLYAYLWLRYHNSN
                     KKLGTREVDDYLAESMAILPFFCGARGVVLWGWEPKSTGQPYRTLPLFMKSLERVAGV
                     SEELGRARPFHDEPVHAAWKARRPLIRRLRVTEDVWLVLATDPWQEEGAERTLRVSCG
                     TRAFDLAVRGKHTEIYRIDGGNAERR"
     gene            11989..14616
                     /locus_tag="PK576_04095"
     CDS             11989..14616
                     /locus_tag="PK576_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE60803.1"
                     /translation="MTGSILLILLLVFYLGGRFILVHMLRNAEKSIQMIGSEVENVVY
                     AEADNLRKRVDSLADAFAAAGESDAETLLQCQVTPQNGDALAHFAALLNTDGSFRKGF
                     FRLPDGQAEPVGAEEMAPYLQTSAPLLNNRLTASHIPTGVIILRGKPILIAMSPIEEG
                     GSGTQGFLLIGTLFSSHLVLDRIHQVTHGMQVAVHDKRVKPDSSDKRFPSAQPNGLAP
                     IFSGALNYHSGGFWHLGENAFEAVIPIHDILGRQVTSISIRLPQTFSSIASIALGWLT
                     VFVASVGIIFVLPMFWLQTRIVLNPLSLLTDQIRKIGERPLDNHTLAIDWPRKDEFGM
                     LAQSVNGMLEALARKSRQVGQIEQQQRALIAGMPDGLCVFDKEARLITIHKQPDSEPP
                     IPGLIVDHPIGPPFFEEKECATLRRAIAKALATEKPQRTLLAYQTPEGAARRLEARVC
                     RMDGARVLVIFRDMTSESREREIRMQMEKRLTKIRKMESLGNLAAGIAHDFNNILAII
                     QNTVEMTWETPDPKTREAVTTICQATDKGAALTRELMTYAGQTSISFKRDDPNALILD
                     LEKLMGGVVAPNVSIELKLAPNLPQVDTDPHQFWKALINLLKNASEAMDGARGHIRIS
                     TYPFTLTEGNLGAFFSTHALSPGNGVVFQIDDTGSGIPREMIAHLFEPFFSTKSVGRG
                     LGLATVFAIIDAHNGGIAIDSDLGKGTTFRIWLPAADKPADEDRAEVRNALQTASPSA
                     LRKTAEAQSPAAPPSIPRVLLIEDDPAILLSTAIVLRSLNVDPLTAATKREALAVFRK
                     QADSIALVLLDAHIDHLDNVRLLSTLRLHNPKVPVVVISGYNEKRIREMFASETYEGF
                     LAKPYTRDELKSMLERFIL"
     gene            complement(14788..15669)
                     /locus_tag="PK576_04100"
     CDS             complement(14788..15669)
                     /locus_tag="PK576_04100"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60804.1"
                     /translation="MAEQEQKMSEAIAFFEQMLQTMPDDRTSLEFLSVAYEQTGQTEK
                     RRDCLIRLADTLLREKDYDNAQVIAGYLSAFTDDPGARAAVERVAELVQGQILKSEFQ
                     REVAGLAGAVSGAAAEVPGSGLPSFQETGVEVHALSRSASAAEMDLVWLWKENEFLPK
                     ETCMDVLHVLTERPVTDTPVLISALALLDEQHPELTERVMESMHRLSEVPPLPIELFE
                     PQPEAVALLPQVFIHVMGVLPFALLGGEVLVAVLNPLNRDLQAEVSARIGKPCHFYLV
                     HPKMWQHVFSKIVPAAV"
     gene            15909..17633
                     /locus_tag="PK576_04105"
     CDS             15909..17633
                     /locus_tag="PK576_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE60805.1"
                     /translation="MPQHIHYPSFQSALLRRGVLSEEQLQKAQEEAGKVPLERFLLQN
                     RLVPPDVFALTAAAYFNISPITLPPGLILPRELLDLASPQLWARLKAAPLLRLGKRLT
                     VAFGDPFDLLAQEKVARVTRLEIVPLVAPEKEVMDVLGRVQSQRDAENPDLAMENIMK
                     VSDADIEFSSEPQHQEENIDKTLETAEEAPVIRMVNMMLVEALRIGASDIHFEAMEKS
                     SRLRYRIDGALIERPSPPKPLHNAIVSRIKIMSDLDIAERRNPQDGRFKVRALKKEVD
                     IRVSILPTIHGEKVVMRTLDKSNLAPSLAALGLDDFAFKAMKFAIDQPHGIILVTGPT
                     GSGKTTTLYSCLQDLNQPDVNIITAEDPVEYQLPGVNQVHVNQQTGLTFASILRSVLR
                     QDPDIVLVGEIRDGETADIAVKAALTGHLVLSTLHTNDAAGVIARLVDMEVPPFLISS
                     SLILAQAQRLYRKLCPICKKPCEVNPDVLAANRIDPAFFDGVTLYEASGCAKCNGIGY
                     KGRGAVMEVLPVTDSIRAAIGRGADSATLRDLAVNEGMVTLKDAGMMKVRNGITSLHA
                     ALEVTGGE"
     gene            17677..18771
                     /locus_tag="PK576_04110"
     CDS             17677..18771
                     /locus_tag="PK576_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE60806.1"
                     /translation="MSEARQKSREIRVSGIGQIRKRELIPFTRQTASMLNAGMSVLAA
                     VSTLEDQCTHPGFRMVLKNLRESIESGSPVSEGLRHFPKIFDDMYVNMVAAGEKSGQF
                     APVLKRLAVMLDSSARLVRKVKSAMTYPAVIMGLAILIAGGLVTFVVPVFAEMFSGFG
                     QKLPAPTRFLVNISDFVRNRWYVLLGVALVAIVGFRKWVATPQGRYTFDKFMLRLPVF
                     GPLTQKVAVARFCRLFAQMLTSGVPILDAMQIVACSMGNKVIESSILAARSGIEQGNT
                     LSASLEGKPNLPLLMVRMIAAGEKSGRIDEMLESVADTYDDEVETTLATLTSLMEPFL
                     MVFLGVVIGGIAIAMFLPIFMLGSVVSGGG"
     gene            18871..20247
                     /locus_tag="PK576_04115"
     CDS             18871..20247
                     /locus_tag="PK576_04115"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE60807.1"
                     /translation="MAKILIVDDEARIRMLLQSLLKANGYEASTAKDGDEALALLKKE
                     EFDLVITDLRMVPMDGMMLFREIKTLFPSMPVILLTAYASVETAIEAMKGGAFDYLTK
                     PFKVDEMLATVRRAEEHAKTHTAVVSTDPNAPLRYQFENLIASNSAMMQVCEMIQRVA
                     PTAATVLINGESGTGKEVIARTIHNTSQRHDKPWVAINCAALPEPLLESELFGHVKGA
                     FTGAHVDKEGLFETASTGTLFLDEISSLPLVLQGKLLRVLQEKEIRRVGDTKNIAVDV
                     RVIAASNTNLEQMVVQGKFRADLYYRFAVITLDIPPLRERLEDILPLARHFIRIESAG
                     SKTLPTISKEAADLLLAYKWPGNVRELENAIKHAVTFLEEDVITPELLPPRIVTRATE
                     AKSQQPLQFPPGSNTSLKGFLKLKEKEYLEQILTSTKGDKEKAAQTLQVSLATLYRKL
                     SDEEPASS"
     gene            20225..>20590
                     /locus_tag="PK576_04120"
     CDS             20225..>20590
                     /locus_tag="PK576_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE60808.1"
                     /translation="MKSQPHPKNADFLQFCKKDLSFLRKADGQPFPVSPKSACVSDLA
                     RCLLNSFRYRQGAGREENECTVVKEQTMKQNRKGFTLVEIMIVVAIIGILAAIAIPNF
                     IKSRKQSQMNACIANLKQIQ"
BASE COUNT         4123 a         6596 c         6047 g         3824 t
ORIGIN      
        1 cattgtcacg gacggttcgg ggcgggtgct cggatgggag gcccttaacg gcgggacgcc
       61 gcttgtcccg gccgtgacgg acgccgccgc gtgggctctg cccgtgctgg aggcgggggg
      121 cgtcgcgttc agcgccgcag gcggcccggc ctcgccgctc gccttcgcgt ccaacgcggt
      181 cgcgacggtc ttccttgcgg tccgcccggc ccactcgccg acgaacttcg ccgcgctgct
      241 cgaggtgccc ggcgcgtccg tgaccgccgt gccccgcgcc gcccagccgg cctacgaccc
      301 cgaggacgcc gccgggctct ccgtgcgggt caacggggag ctctcgctgg cctttcccgc
      361 cgcgccgcat atcgtcgagg tggacttccc cgaacccgtt tccggcggcg agctgcgcat
      421 cggcggcggg tccgcctgcg ccgcgtggcg gcagcagtgg cgcgggaacg tcgccggggt
      481 catcgcgttc ggcgcgcccc cgagcgaatc cgtccgccag gtcgtgcgca gctatctcgc
      541 ccgccgccac ggcatcgcgg gggccttccc cccgccggga ggcggggcgg tgctccaggc
      601 catggcgcaa ggggtggatt tgcacggcct cttttcgacg cggctgttgc tgtggtgacg
      661 gccatcgccg ccgcgctgtg tgcggatacg gctcgaacgc acaacgttca ggctatcgct
      721 aaggacggcg cccagaaccg tcggcggacg cctcgggaat gtgtccctgc cggagcccgg
      781 cacggcttcg gcagtccggc tccgcgcctg gctcgcagct gtggacgtcc cgccgtaccg
      841 gcagggaccg tctctgcttg cccgtgtttc atgagcggga cgcgccaatg cgttccactg
      901 ccccgatacg ccgttgacct ttgccggagg gtaccgcata cttctgaatg accgtgcatg
      961 cggcgcggtg aacgggagat ccggaatgga gtgtcaatcg taccgtcagg gatgttgcgg
     1021 ctgttgcgtg aacatgcgtt ggggcgacgc aaaaatcgcg gcctttctgg aggcgaacac
     1081 gcgagccgcg gaacagcttt tcccgcctca cggcgcacgg ccccggtacc gcgatttggt
     1141 acgggcgcat ctgcggtgcg gcggatggcg tgaccatctt ctgatgaccc tgctcgctcc
     1201 gctcacactg gggctcgcag cctggtattg gaagcggcgg atggggagtt gttgcttcgc
     1261 cgggtttctc gaccgcgcgt cgggccgtgt gggttgtctg atccatccgg cacgggcggg
     1321 aacacctgat ctgcgtcggc acgccttccc gctgatcccc acactcggat gcgaccgcgc
     1381 gctacgctgc cccatgctcg atgcggagaa tccggacccg gacctcgact ggctcacggt
     1441 ctcgcgccgg ggcgccgcga gccttgacgg ggcccatccc ccttttcctg accgtgtaat
     1501 cggtcagtga cggggggggg aagagtcatc gtgtacgggt acggggtatg gccaacgttc
     1561 acgttctcca aatcgggatc ggtatcgcta tcgggttcgg tatcgactgt tgcgcccacg
     1621 cagtggcccg cagtgacaag tgtaatgatg gcggcgattc cgatcccgat cccgatggcg
     1681 ccgcaatgga accctgaaac gcatgacctt cgatcgcacc cctgtcacta acgcgggcga
     1741 agcccgcaac ccaagttaat tagtgcgtta gttaattagt gcattagttt gtctgggagc
     1801 gcgttacctc caaaaaacct tagtgccctt tgtgccttcg tgtgataaaa aaaccgagta
     1861 ccccccctgc cactaaccga ttacgttatt ccgttattca ctgcgttttc cattgccttg
     1921 ccgcctcatc tcctctatac tgtatcccat gaacgcactg gtgtcggtgt cgtgcgatga
     1981 gtatcgcaca gttcggcagg tgcgataaaa aacgagttgt taaaagaagg ccgtcatgat
     2041 gtgtaacgcc atgaaccaca acaccgtgtt ccgcaccgca cgttcgtccc gctcaccgcg
     2101 tctgtgcgcc ttcgcgctcg cgcatctcgc gttgctcctc ccccttggcg tgtccgccga
     2161 cacctacggc gactacacct acaccgtcag cgacggcgga gccaccatca ccaagtacaa
     2221 cggctccggc ggtgccgtcg ccatcccttc caccttcggc agccatcccg tcaccgccat
     2281 tggtaattac gccttctccg aacacacact cattacctcc gtcaccatcc ccgacagtgt
     2341 caccaatatc tgcgactggg ccttcctact ctgctcctcc ctcgcaaccg tcaccatcgg
     2401 taacagcgtc accaacatcg gcggatacgc cttcgcagaa tgcccctccc tcaccgccat
     2461 gaccatcccc aacagcgtct ctgatatcgg tacccacgcc ttcgcagaat gcccctccct
     2521 caccgccgtc accatcggca acagcgtcac taacatcggc ggatacgcct tcgccgactg
     2581 cccctccctc accaacatcc tcttcaccgg cgacgccccg gccctcggct actccgcctt
     2641 ccataataat aatcccgcca cgttctacta cctgcccggc atcggcgact ggccgtcctg
     2701 gttcggcggc cgtcccaccg tctgctggaa ccccgccttc agctcggcgt cgtccgcctc
     2761 cggcgcgttc gccgtcaccc tcaccggtaa cccctccgcc gccctccccg tccgcatcga
     2821 agccagcgac agcctctcgt ccccctcctg gctccctctt tccggcacca ccaccatccc
     2881 cgccggcggc accctcaact tcaccgatcc cgacgccagc ccccctcccg cgcgcttcta
     2941 ccgcatcacc ttcccccgat agaggggaag ggaagtgcat gagggcatga atgcatgaat
     3001 gcgtgacgtt tcgacgagag tgacgggggg aggagagtga cggggggagg agagtgacgg
     3061 ggggaggagt tatcgtgtac gagtacgtga acggggtata gccaacgttc acgttctcca
     3121 aatcgggatc ggtatcgcta tcggtatcgc tatcggaatc ggtatcgcta tcgggttcgg
     3181 tatcgactgt tgcgcccacg cagtgcagtg gcccgcagcg acaagtgtaa tgatggcggc
     3241 gattccgatc ccgatcccga tggcgccgca atggaaccct gaaacgcatg accttcgatc
     3301 gcacccctgt cacgaaccga ttaccctgac cgttgaggtt tctggttaca ggttttcggc
     3361 agatgatgta tgatatcttg taaatgttga aacaacgaat gcgggcagag cccgcgacct
     3421 cacttcgtat cacacaaaga ctcaaaggac acaacggttt tttcatgatc ccttcatgtg
     3481 agaaacagct tgttttttta ttggccttat cggtcgataa ggcaccaaag gaggatggat
     3541 atgtcttttg aaatgtcccg gaaagggttc cttggtatgg gtggttcgtt tttcgccgcg
     3601 aacgtattca gcgcaacggc gcagaaggac aaggacggca aggtcatcgc gggtttcgat
     3661 gagacggacg caggcaaaat cgcgcaggtc aagtgggaac cgttctccga caagaaggtg
     3721 cgcgtcggca tcgccgggca cggcatttgc aaattcggct gctcgttcgg ctaccagaac
     3781 catccgaacg tcgagatcgt cgcgtgtacg gatctcgatc ccgcgcgttg cgccgacatg
     3841 cagaagcaac tcaaggcgcc gcggactttc aagagttgcg aggagatgat caaggagatg
     3901 cgcggcaaga tggacgcggt ctacatcgcg accgatccgc cgagccacgc gccgcttgcg
     3961 atcctggcaa tggagaacgg catgcacgcg gtctcggctg ttcccgcatt ctggggcgac
     4021 attcaggagg agtacgcgca gaagctcgtc gataccgtca agaaaacggg gatgatctac
     4081 cagatgaacg agacgaccgc gttccgtccc cagtgttttg cgatgcgcgc gctctacaag
     4141 ggcggcgcgt tgggcgaaat catctatacg gaaggcgagt actggcatca tggctcaaga
     4201 ccgatgggca gttacaaagg ctggcgcaat ttcgccgcac cgcagtggta ctgcacccac
     4261 ggcaacggct tctacaactg tgtcacccac ggacacttca cgaaatgcgc atgtctgggt
     4321 gtgcccacgc actatgatgc caagaagtcg ttcatcggca agcccggcaa ctacggcaac
     4381 atctacggta cggaagtcgc gcttctcaag acatccgagg ggggaatggc ccgcatggga
     4441 cgcggcggag atacgccgtc cgccatccaa gagacaggac gctgcctcgg cacacgcgga
     4501 caatacgacg agggttacaa gggtgaggca acgaaggaag agcttgccaa gattgcctac
     4561 aataagccgc cgttgccgcc gggagtgccg gcaggcggac acggaggcag ccacggttat
     4621 cttacggatg acttcatccg tgcgatcctg ctcggccaga agccggtcgt tgatgtcatc
     4681 tgcgcggtca acatgaccat cgccggaatc tacatgcatc gctcggcgca gaaggacggc
     4741 gaatggctga ggattccgca cttcgccatc tgactctgta ggccacaggc aaaccgcccg
     4801 aattggtgtg tcgcgcctga cgggattgac tcccggggcg cgtgtggtac actgtctccc
     4861 gttttaaacc aacaaggaaa aaaccgtgaa aaaacctgtc tctgtgaaaa cgtcccgcgc
     4921 gaggaagtct gcggggaaaa cggcaaaatt cgtttattcg ttcggttcgg ggcgctccga
     4981 gggttcggtc tccatgaaga ccctgctcgg cggcaaaggc gcgaacctgg ctgacatgtc
     5041 cggcctgggt ctgccagtgc cccccggttt cacaatcacc acggaagcct gcgcgaagta
     5101 ctatgaggtc ggcgagaagg ctttgcacgc gttgctccgc gatcaggtga cggaagcatt
     5161 gcgcaaactc gagaaagcca ccggcaagac gttcggccac ggccccaacc cgttgctggt
     5221 ctccgtgcgt tcgggtgcgg cagcctccat gcccggcatg atggatacgg tcctcaatct
     5281 gggccttaac cccaagacgg tcgaggcgat gatcgagcgc acgggcaacc cgcgcttcgt
     5341 ctgggattcc taccgccgct tcattcagat gttcggcaac gtggtcatgg gcgtggagca
     5401 ccacgagttc gagcgggaac tggagaacgt gaaacacgcc agagggtaca cgctcgacac
     5461 ggaactcatg accgaagacc ttcaggatgt tgtggcgcgg tatatgaaac gggttcggga
     5521 gatcaaaggg tgcgccttcc cgaccgacgc gatggagcag ctctggcgcg gcattgacgc
     5581 ggtgttcggt tcctggaaca acccgcgggc catcaagtac cgggcgttga acgacatccg
     5641 cgggcttctc ggcacagccg tgaacgtgca aaccatggtg ttcggcaact ccggctccaa
     5701 ctccgccaca ggcgtggcct tcacgcgtga cccctcgact ggcgagcaca agtactttta
     5761 cggcgaatac ctgatcaacg cgcagggcga ggatgtggtg gccggaatcc gcacgccgca
     5821 gcagatcacg atccggggtt cgcgcgaatg ggccaaggcg cgcaacatca ccgaggcggt
     5881 gcgcaaggtg cagtacccgg cgctggaaga ggccatgccc aaggtcttca agcagcttga
     5941 cgcgatccgt gtcaagctgg aacggcatta ccgtgacatg caggacctcg aattcacgat
     6001 cgaggacggc aagctgttca ttctgcagac ccgcagcggg aaacgtaccg cggctgcggc
     6061 ggtgaagatc gccaccgatc tcgtccggga gaagatcatt gacgaaaaaa cggcggtgct
     6121 gcgcgtcgaa ccgggtcagc ttgatcagct cctgcatccg gtctttgtgc gcagtgccga
     6181 ggccaaggcc aaaaagatca ccgtgggctt gccggcctcg ccgggggcgg ccaccggtca
     6241 gatcgtcttc aacgcggcga ccgccgaact gtgggcccgg gagggaaaga aggtcattct
     6301 ctgccgtatc gagaccagcc ccgaagatat cggcggcatg aatgtcgcgg ccggcatcct
     6361 gaccgcgcgc ggcggcatga cctcgcatgc ggcggtggtg gcccgcggca tgggtaaatg
     6421 ctgcgtctcc ggttgcggcg aactgcacat cagttacgac aagaaaacca tctcctgcaa
     6481 caagctggtg ctgaaggagg gcgactggat ctcgctgaac ggctcgaccg gcctggtgta
     6541 cgaaggccag atcgagaccg aggatccgaa gctcaccggc ccgttcgcga tgatcatggc
     6601 gctggcggac aaataccgca agctgggcgt gcggaccaac gccgacacgc cgcgcgacac
     6661 cgccaaagct gtggcgttcg gtgccgaagg cgtcgggctc tgccgcaccg agcacatgtt
     6721 cttcgagggg gaccgggtga tcagtttccg ccgtatgatt ctggtggccg aaaccgtcaa
     6781 gagcctgcgg caggcgctgg ccgagtgtac ggacccggaa gagcgtaccc gtctggaaca
     6841 ggagttggct gcgccgctga aacaataccg gcaggcgctc aaagaactgt tgccgctgca
     6901 acgtaaggat ttcatcagca tgttcaaggc gctcaaaggg cgcccctgca cggtgcggtt
     6961 gctggacccg cccctccacg agttcctgcc gcatgacgag gccggacagg ccgaaatggc
     7021 gcgggagatg gaccttgagg tcatccagat ccgcgaggtg gtcgagtccc tgcatgagac
     7081 caaccccatg ctgggccacc gcggttgccg tctcggcatc agctatccgg aggtgaccga
     7141 gatgcaggcg cgtgcgatct gcgaagccgc ggctgcggtc aaaggctcgc agcccgagat
     7201 catggtgccg ctcgtcggta acgtcaaaga gctgaccgcg caaaaggaac tgatccacaa
     7261 ggttctggct gatgtggaac ggacccggaa agtcaaactg ccgatccgga tcggcacgat
     7321 gatcgaggtg ccgcgcgcag cagtcaccgc agaccagatc gcggcggagg cggacttctt
     7381 ctctttcggc acgaatgacc tgacgcagat gacctgcgcc ttctcacggg atgacgcggg
     7441 caagttcctc ggcgagtatg tcgcgcgcgg gttctatgat tacgacccgt tccaagtgct
     7501 ggaccaggag ggcgtgggcc gtatggtcga aatcgcgatg cagtacggcc gcggcgtgaa
     7561 gccgaatcta aaggtcggtg tctgcggtga gcacggcggc gatccgaaga ccgtggagtt
     7621 ttgccacaaa ctcgggttca gctatgtgag ctgctcgccc ttccgtgtgc cgatcgcgcg
     7681 tctggctgcg gcacaggccg cactgcgaag ctgaggcgcg ttccgctccg gccgccctca
     7741 tgcgcttccg ggcaaagagg gggtggccgg tgacggaaaa cgcggcctcg ttctttgggt
     7801 tacggttgtg cggcgggctc gcggatgcgg atggatcgga accacgtttc atccgtatgg
     7861 tcggtcagca tgatgtgccc cttgatcttg tcgccgaaat cgggaacctc ggcaaacttg
     7921 ctggctgcca cgcccgcctt gacttcggcg ctgccgaaga cgtactccaa aaccttccgg
     7981 ccgttgaggc tgtgctcgca ccggtcgccc cgcacaacga tgcgggagac attccactcc
     8041 cccgccggtt tcagcggttt gtcggccgca ggcggcaaca ccgcatagaa cgaggcggtc
     8101 cgcgcattcg ccggcagttt cacccatttc gccgagagat cgtcgatcat ttggtactcg
     8161 tagcccggcg cgccgggacg cgcttcggtg acgcagtact tcaccccgtt gtttccgccg
     8221 ggagagatcc gccactccca ctccagttca aaatccccgt acgttttctc cgtgatcagg
     8281 tcgcctcccc tgatcttggg ttttttgcgc agcagtcccc cctcaaccga ccatccctcg
     8341 ccgggcgccg ttcccaccgg tttgccgtac ggccgccatc cggccaggct cttgccatcg
     8401 aaaagctcaa cccaaccatc ctgtccggcg cgggactcca gcgcggtttc gatcgccctg
     8461 accacttcgc ccgccaatgc cttggaccct gcggcagtgt aatgcacatc gcgggggcgc
     8521 cctcccatag cgaggcgccg ttcgggcgga ccgacgaacg cgtgcagatc gttgaccggc
     8581 acccccgcac cccgcatgac gcgcagcgcc gcttcgttgt aggcgacctc actgcccgcg
     8641 atacggatcg ccgtcccctc cggaaccggt gtggtcgaac accagatcaa ccgtgcgccg
     8701 gttgttttga gccgccgcac cagcgtgccc agattcgtct cgtactgttc caccggaacc
     8761 cactgacggc ccttggcgac atccgtagcc ttgccctgat cgtcgatata tttgaggtcg
     8821 tgcagccccc agttgaaatg gatcacatcc catttgccgg taccgagcca cgtttccaga
     8881 tgcgcgaggc ccgtggcggt cgcctgattg ttgcccggcg cgtgcacaat ttccatccgg
     8941 ttcgagagcg ccgccttgac gaacggcgtg tacccgagcg aaatcgagtc gccgatgatc
     9001 aatacgcgag gccgcgactc cgtctgcgcg cgcaccgcgc acagcgctcc ccctgccatc
     9061 acagcgcaaa ccgccaaccc aatcatcttg ttttttgtcc cggtcatccc gtccctcccg
     9121 ttcccgcact ggcgctgaac gcattgaaca ctccagccag ccccgtttcc acccgagcgg
     9181 aaccccaggc gggcagcgtg tctcttttgt gtggcggaca gtgtagcaca cccctcaacc
     9241 gagcacaatt ccttgctcgg aaacgggaga ccggaatgcc cttgaaaaag agggttgact
     9301 gttaccatac ccccgccaat aataaagcac tgcaacaggg tgggggcgta ttttgttatc
     9361 cggccggacg ggatttcccg gctgaacgtc acaaccccga aacggaaaca cgcatcatga
     9421 tccgaaaatc actcatcacc ggcggtgccg gattcatcgg ttcccatctc gcgcggcggc
     9481 tgctggccgc agggcacgag gtcaccgtca tcgacaacct cttcacaggg accaaggcca
     9541 acatctacga cctgctggac aaccgccgct tcaccttcat cctgcatgat gtgacgcagc
     9601 ccttctgggg acagttcgac gaggtgtaca acctcgcctg tccggcctcg cccatccact
     9661 accagcgcaa tcccgtcgag accctcaaaa cctcctttct cggcatcatg aatgtgctgg
     9721 agcttgcgct caagaccaaa gcccgggtat tccatacctc cacctcggag atctacggcg
     9781 acccggaaat ccatccccaa ccggaatcct actggggcaa cgtcaactcc atcggtgcac
     9841 ggagttgtta cgacgaaggc aagcgcgccg ccgaaaccct gtgcgccgac tacgtgcatg
     9901 agtacggcgt ggacgtgcgt atgatccgca tcttcaacac ctacgggccc aacatgcatc
     9961 cgcaggacgg ccgcgtcatc agcaatttca tcatgcaggc attgcggaac caggacatca
    10021 cgctatacgg cgacggctca cagacccgca gcttccagta tgtcgacgat ctgatcgacg
    10081 cgatcctgcg cttcatggcg ctggaccaca aaaaagtgaa aactttcttc gaaggccggg
    10141 ccatgcgcgt gccggtcatc aacatcggca accccggcga gtacaccatc cgccaactcg
    10201 ccgaggagac cctgcggctt ttacccaaga gccggagccg actggcgttc catcccctgc
    10261 cgaaggacga ccctcggcgg cgcaaacccg acatcacgct ggcgggtgaa ttgctcggct
    10321 gggcgccatc ggtcgccctg cccgaggggc tggccaaaac gatcgcttat ttccagaacc
    10381 tcgtttgaaa agaaaggggg aaatcccgcc atgcccttcc cgcaagccgt tcttccgctt
    10441 ttaaatctcg tctgcggctt ggccttgggc gccgcgttgt ccgttgcggc gcaaacggag
    10501 cagcccccgc ctccgagttg gggagagatt ggccgcgtca acatgcgggg cggcctgagg
    10561 atcttctgga acgtgggcgg caaagaccac aaggccaacg agcgcgaagc tctggcgcgc
    10621 gggttcgaac ccgtcgacct gttgaacacc tactctgact accccggaaa gcaacgggaa
    10681 aagatcatcc cgaacgggaa gaatccttgg gccaagccac cgcaattcga gcgcatcatc
    10741 cgccgcaaca tcgccatgaa acagggacgc ggactgtttg tccacgacat cgagtttgac
    10801 ttcgatgagg agccggaggc cctctggacc aatccggtat tgcgggccgc atcgggcgcg
    10861 gacacggtgg aggcgttccg caaggcctac taccgcgagt ggggcacctg gtataccctg
    10921 ccttgccgct gggccaaaga ggcctttccg caccttccgg tggggatcta cggcccgcag
    10981 cccttccggc gcgactactg gggaatctgc ggcaaggacg cgcaacagat cgacggcacg
    11041 cacaaaaccg atgccgaatt gtggcagcac atcgatccgg cggtggattt ctgcatcgcg
    11101 agcctctact gtttttatga ccgcccggac tcggtcttct acatgggcgc caacgtggag
    11161 gagaacgcgc aacggatggc gctgaccgga gacaagccac tctatgccta cctctggctc
    11221 cgctaccaca acagcaacaa aaaactcggc acacgcgaag tcgatgatta tctggccgag
    11281 tccatggcca tcctgccctt tttctgcggg gcgcgcggag tcgttttgtg gggatgggaa
    11341 cccaaaagca cgggacagcc ctaccgcacg ctgccgcttt tcatgaagag tctggaacgg
    11401 gtggccggcg tgtcggagga actcggccgc gcacgtccgt tccatgacga accggtacac
    11461 gccgcctgga aagcgcgacg gccgctgatc cgcaggctgc gcgtcacgga ggatgtctgg
    11521 ctggtgctcg cgaccgatcc ctggcaggag gagggcgcgg aacgcacgct ccgcgtctcc
    11581 tgcggcacac gggcctttga tctcgcggtc cgcggcaaac acaccgagat ctaccggatc
    11641 gacggcggga acgcggaacg ccgctgacgc gcgggacgcg ggagacataa tcggttcgcg
    11701 acgagggtgc gaaaggaaaa cttgttttca tcgcaccttt tcagcttttt ggcccgattt
    11761 atgctttact attgatgctt tatcaacatt cccgcctccc gattttttgg ggaaacccga
    11821 gtggcaaccg gaggaaagag agtgcggaac gcgttcacaa acccggcgtc ccttttttgt
    11881 ctcattctgg aaaagggaca gacaagatac gattcgctgt tgcgcaaccg tatgaaggca
    11941 tccgcgatag tcagggattt acggagcatc cgaaccaagt tcagtctggt gacaggatcc
    12001 atcctgctga tcctgctgct ggttttctat ctgggcggac gtttcattct cgtgcacatg
    12061 cttcgcaatg ccgagaaaag catccagatg atcggttccg aggtggagaa tgtcgtttac
    12121 gccgaggccg ataacctgcg gaagagggtg gacagtctag cggacgcgtt tgcggcggcg
    12181 ggagaatcgg atgcggagac cctgctgcag tgtcaggtca cgccgcaaaa cggggatgcg
    12241 ctagcgcatt tcgcggcgtt gctgaatacg gacgggagtt tccggaaagg gtttttccgg
    12301 ttgccggacg gacaggccga accggtcggg gcagaagaga tggccccgta tctgcaaacg
    12361 tcggctccgc tgctcaacaa ccgcctcacc gcctcgcata tcccgaccgg cgtgatcatc
    12421 ctgcggggga agccgattct cattgccatg tcgcccatag aggagggcgg gagcggaacc
    12481 caagggtttc tgctgatcgg caccctcttc agcagccatc tggttcttga ccgcatccac
    12541 caggtgacgc acggcatgca ggtggccgtt catgacaaac gtgtcaaacc ggactcttcg
    12601 gacaagcgct ttccgtcggc gcaacccaac ggacttgcgc cgatcttctc cggagcgttg
    12661 aactaccact cgggcggatt ctggcatctg ggcgagaacg cctttgaggc cgtgatcccg
    12721 atccacgaca tcctgggccg acaggtcacc tccatctcga tccggttgcc gcaaaccttc
    12781 tcctcgatcg ccagcatcgc actgggatgg ctcactgtgt ttgtggcgtc ggtgggcatc
    12841 attttcgtgc tgccaatgtt ctggctgcag acgcggatcg tgctgaaccc gctgtccttg
    12901 ctgaccgacc agatccgaaa gatcggcgag cggcccctcg acaaccacac cctcgcaatc
    12961 gactggccgc gcaaagacga gttcggcatg ttggcgcaga gtgtcaacgg catgctggag
    13021 gccctcgcgc gcaagtcgcg gcaggtcggg cagatcgagc agcagcagcg agccctgatc
    13081 gcgggcatgc cggacggcct gtgcgtgttc gacaaggagg cccgtctgat caccatccac
    13141 aaacagcccg acagcgagcc gccgatcccg ggcctgatcg tggaccatcc gatcggtccg
    13201 cccttcttcg aagagaaaga atgcgccacc ctgcgtcggg cgatcgcgaa ggccttggcc
    13261 acggaaaaac cgcaacggac cctgctcgcg tatcagaccc ccgaaggtgc ggcacgccgg
    13321 ttggaagcgc gcgtctgccg gatggacggg gcccgcgtgc tggtgatttt ccgcgatatg
    13381 acctccgaga gccgtgaacg cgagatccgg atgcagatgg agaagcgcct gaccaagatc
    13441 cggaagatgg agagtctcgg aaatctggcg gccggcatcg cgcatgactt caacaatatc
    13501 ctcgcaatca tccagaacac ggtggagatg acgtgggaaa cgcccgaccc gaaaacacgg
    13561 gaggcggtca caaccatctg ccaggccacg gacaagggtg ccgcgctgac gcgggagttg
    13621 atgacctacg ccggccagac gagcatctcc ttcaaacgcg acgatcccaa cgccctgatt
    13681 ctcgatctgg agaaactcat gggcggcgtg gtcgccccca acgtctccat cgaactgaag
    13741 ctagccccga acctgcccca ggtcgacacg gacccgcatc agttctggaa agccctgatc
    13801 aacctgctca agaacgcctc cgaggcgatg gacggtgccc gcggccacat ccgcatcagc
    13861 acttacccgt tcacgcttac cgaaggcaat ctcggcgctt ttttcagcac acacgcgcta
    13921 tccccaggca acggagtcgt attccagatc gacgacaccg gttccggcat cccgcgcgag
    13981 atgatcgccc acttgttcga acccttcttc tcgaccaaat ccgtgggccg cgggctgggg
    14041 ctggcaacgg ttttcgcgat catcgacgcg cacaacggcg gcatcgccat cgattccgac
    14101 ctcggcaagg gcacgacctt ccgcatctgg cttccggctg cggacaaacc cgcagacgaa
    14161 gaccgcgcgg aggtccggaa cgcccttcag acggcatccc cctccgcact ccgcaaaacg
    14221 gccgaggcgc aatccccggc cgcgccgccg tcgatccccc gtgtcctgct gatcgaggac
    14281 gatccggcga tcctgctcag caccgccatc gtcctgcgct cgctgaacgt cgacccgctg
    14341 accgccgcca ccaaacgcga ggctctggcg gttttccgaa aacaggccga ctccatagcc
    14401 ctggtgttgc tggacgcgca tatcgaccat ctcgacaacg tgcggctgct ctcgaccctg
    14461 cgcctgcaca acccgaaagt gccggtcgtg gtgatctcgg ggtacaacga gaaacgtatc
    14521 cgggagatgt tcgcctccga gacgtacgag ggcttcctgg ccaagcctta cacccgcgac
    14581 gaactcaaga gcatgctcga acgctttatc ctataataaa taataaacgg caggtgagcg
    14641 tgcgaactcg ttttcgtgta cgtggacttg cgacccgcga cattcgactt gcggcgtttg
    14701 ggttcccggc aagacgaccg atccctccga tccgcctttg cgggcgaaga cgcccgtttt
    14761 tgattggccg tatcgaccga caaggcgcta gacagcagcg ggcacgatct tggaaaacac
    14821 atgctgccac attttggggt gcaccagata gaagtggcac ggtttgccga tgcgcgcgga
    14881 cacctcggcc tgcagatccc ggttcagcgg attcagcacc gcaaccagca cctcgccgcc
    14941 aaggagcgcg aaaggcagaa cccccatgac atggatgaac acctggggca gcagcgcgac
    15001 cgcctccggt tggggctcga acagttcgat cggcagaggc gggacctcgc tcaaacgatg
    15061 catcgactcc atcacccgtt ccgtcagttc cggatgctgc tcgtccaaca gcgccagggc
    15121 cgagatcaaa accggcgtgt ccgtcaccgg acgctccgtc agcacatgga gcacatccat
    15181 gcaggtctcc ttcggcagga actcgttctc cttccagagc caaaccaaat ccatttccgc
    15241 cgccgaggcg gagcgcgaaa gcgcgtgcac ctcgaccccg gtctcctgaa aggacggcag
    15301 accgcttccc ggcacctccg ccgccgcacc ggacaccgcg ccagccagcc cggcaacctc
    15361 ccgctggaac tcgctcttga gaatctggcc ctgcaccagc tccgccacgc gctccaccgc
    15421 ggcgcgtgcg ccggggtcat ccgtaaacgc gctcagatac ccggcgatca cttgggcgtt
    15481 gtcataatcc ttctcgcgca acagcgtatc cgccagccgg atcagacaat cgcgccgttt
    15541 ttccgtctgg ccggtctgct cataagccac ggacaggaac tcgagactcg tccggtcatc
    15601 cggcatggtc tgaagcatct gctcgaaaaa cgcgatcgcc tcgctcattt tctgttcttg
    15661 ttccgccatg tcagcctaaa ctccttctgc ttttttattt aagcagaaaa catgccgacc
    15721 cggatttccg ggcagaaaga gggcgcgccc ctgttgaccg gatcgattct ggcaccttat
    15781 ctgctaatag atgttacgcc tctttcggtc aggggagtac tcgaacaacc ggtgcaaatg
    15841 tccgggaaag cgctcctgcc gcagatggca tggacaaggg acagaatacc ggaaaaccgg
    15901 aaaaaacgat gccgcaacac atccattacc ccagtttcca gagtgcgttg ctccgacgcg
    15961 gggtgctctc ggaagagcag cttcagaagg cacaggaaga agcgggcaaa gtcccgctgg
    16021 agcggtttct gcttcagaac cggctggttc cgccagatgt gttcgcgctg acggccgcag
    16081 cctatttcaa catttcgccc atcaccctgc cgcccggcct gatcctgccg agggagttgc
    16141 tcgatttagc ctcgccccaa ctctgggcca gactgaaagc cgcgccgctc ctgcgtctcg
    16201 ggaaacggct gaccgtggct ttcggagacc cgttcgatct gctggcccaa gaaaaggtgg
    16261 cgcgggtcac gcggctggag atcgtgccgc tcgtggcacc ggaaaaagag gtgatggacg
    16321 tgctggggcg ggtccagagc cagcgggacg cggaaaatcc cgatttggcc atggaaaaca
    16381 tcatgaaggt gtcggacgcc gacatcgagt tctccagcga gccgcagcat caggaagaga
    16441 acatcgacaa gacgctggag accgcggagg aggcgccggt catccgcatg gtgaacatga
    16501 tgctggtcga ggccctgcgt atcggtgcca gtgacatcca tttcgaggcc atggaaaaaa
    16561 gctcccgcct acgctatcgg atcgacgggg cgttgatcga gcgccccagc ccgccgaaac
    16621 cgcttcacaa cgcgatcgtc tcgcgcatca aaatcatgtc ggacctcgac atcgcggagc
    16681 ggcgcaaccc gcaggacggc cggttcaaag tccgggcact caagaaagag gtcgatatcc
    16741 gcgtgagcat cctgcccacc attcatggcg aaaaggtggt gatgcgcaca ctcgacaaga
    16801 gtaacctcgc ccccagtctg gcggcactcg gcttggacga tttcgccttc aaggccatga
    16861 agttcgcgat cgatcagcca cacggcatca ttttggtgac cggcccgacc ggatcgggca
    16921 aaaccacaac gctttactcc tgcctgcagg atttgaacca gccggacgtc aacatcatca
    16981 cggccgagga tcccgtggag tatcaactcc ccggtgtgaa ccaagtccat gtcaaccagc
    17041 agaccggcct gactttcgcc tcgattctgc gctccgtgtt gcggcaggat cccgacatcg
    17101 tgctcgtcgg tgaaatccgc gacggcgaga ccgcggacat cgcggtcaag gccgcgctca
    17161 cgggccatct ggtgttgagc acgctgcaca ccaatgatgc ggccggcgtg atcgcccgcc
    17221 tggtcgatat ggaggtcccc ccgttcctga tctcctcctc cctgatcctg gcccaggccc
    17281 agcgcctcta ccgtaaactc tgcccgatct gcaagaaacc gtgcgaggtg aacccggacg
    17341 tgctcgccgc caaccgcatc gacccggcct ttttcgatgg cgtaacgctt tatgaggcca
    17401 gcggctgcgc caaatgcaac gggatcggct acaagggacg gggtgccgtg atggaggttc
    17461 tgcctgtcac cgattcgatc cgtgccgcca tcggccgcgg cgcggactcg gccactctgc
    17521 gtgatctggc agtcaacgag ggcatggtca cgttgaaaga cgcgggcatg atgaaagtcc
    17581 ggaatggcat cacctcactc catgccgcgc tcgaggtgac cggcggagaa taatgacacg
    17641 cccgccctgc gggccatccg ggaaagggag tcgacgatgt cagaagccag acagaaaagc
    17701 cgcgagatcc gggtatcggg gatcggacag atccgaaaga gggagttgat ccccttcacg
    17761 cggcaaaccg ccagcatgct gaacgccggc atgtcggtct tggcagccgt ctcgactctg
    17821 gaggaccaat gtacccaccc cggattccgc atggttctga aaaatctccg cgagagtatc
    17881 gagagcggct ctccggtctc cgaagggttg cgccacttcc cgaaaatttt cgatgacatg
    17941 tacgtcaaca tggtcgccgc gggtgaaaag agcggacaat tcgcacccgt gctgaaacga
    18001 ctggccgtga tgctcgactc ctccgcgcgc cttgtgcgca aggtcaaatc ggccatgacc
    18061 tatccggccg tgatcatggg tctggccatc ctgatcgcgg gcgggctcgt caccttcgtc
    18121 gtaccggttt tcgccgaaat gttctccggt ttcggccaga aactgcccgc ccccacccgg
    18181 ttccttgtga atatcagcga tttcgtccga aaccggtggt atgtgctgct cggggtggcg
    18241 ctcgttgcca tcgtcgggtt ccgcaagtgg gtcgccaccc cgcagggccg ctacaccttc
    18301 gacaaattca tgctgcgtct cccggtcttc ggcccgctca cccaaaaagt cgctgtggcg
    18361 cgtttctgcc gactgttcgc gcagatgctc accagcggcg taccgattct ggacgccatg
    18421 cagatcgtcg cctgttcgat gggcaacaaa gtcatcgaaa gctcgatcct cgcggcccgt
    18481 tccggcatcg aacagggtaa tacgcttagc gcttcgctgg aggggaagcc caatctaccg
    18541 ctgcttatgg tccgcatgat cgccgcaggc gagaagagcg gccggatcga cgagatgctt
    18601 gaaagtgtgg ccgacaccta tgatgacgag gtcgagacca ccctcgccac cctcacctcg
    18661 ctgatggagc cgttcctcat ggtcttcctc ggcgtggtca tcggcggcat cgcgattgcc
    18721 atgttcctgc cgatcttcat gctcgggtcg gtggtcagcg gcggcggatg atccccgccc
    18781 cgaatcccga aaacgtgttc ttgagttcgg taccccctta tcctatacta agttgacttt
    18841 tatcgggtac ctgcgtggac atgtttcaat atggctaaaa ttctgattgt cgatgatgag
    18901 gctcggatcc gtatgctgtt gcaaagcctg ctgaaggcca acggctatga agcatccaca
    18961 gccaaggatg gcgatgaggc tcttgcgctc ctgaaaaagg aggagtttga tctggtcatc
    19021 actgacctgc gcatggtccc catggacggc atgatgctct ttcgtgagat caaaaccctc
    19081 ttcccctcca tgccggtgat cctgctgacc gcctatgcgt cggtcgagac cgcaatcgag
    19141 gcgatgaaag gcggagcctt cgattacctg accaagccct tcaaggttga cgagatgctc
    19201 gccacggtgc gccgggccga agagcacgcc aagacgcaca ccgccgtggt ctcgaccgac
    19261 cccaatgcgc cgctgcgcta ccagttcgag aacctcatcg cctcgaacag cgccatgatg
    19321 caggtctgcg agatgatcca gcgcgtcgcg cccaccgcgg ccaccgtgct catcaacggg
    19381 gaaagcggca ccggcaaaga ggtcatcgcg cgcaccatcc acaacaccag ccagcgtcac
    19441 gacaagcctt gggtcgccat caactgtgcg gcgctccctg agccgctgct cgaatcggaa
    19501 ctcttcggac acgtcaaagg ggccttcacc ggtgcccatg tcgacaaaga aggactgttc
    19561 gaaaccgcca gcaccggcac actgtttctg gacgagatct catccctgcc tctggtgctg
    19621 cagggcaaac tgttgcgcgt gctgcaagag aaagagatcc ggcgcgtggg agacacgaag
    19681 aacatcgcgg tggatgtgcg ggtgatcgcc gccagcaaca ccaatctgga gcagatggtg
    19741 gtccagggca aattccgggc cgacctctat taccgtttcg cggtcatcac cctcgacatc
    19801 cccccgttgc gcgaacggtt agaggatatt ttgccgctgg cccgccactt catccgcatc
    19861 gaatccgcag gctccaagac attgcccacc atctccaagg aggccgccga tctgctgctc
    19921 gcctacaaat ggccgggcaa cgtgcgcgaa ctggagaatg ccatcaagca cgcggtgacg
    19981 tttctggagg aggatgtcat cacccccgaa ctcctgcccc cgcgcatcgt cacccgcgca
    20041 acagaggcta aatcccaaca gccccttcaa ttccccccgg gcagcaacac atcgctgaaa
    20101 gggttcctta aactcaagga gaaggagtat ctcgaacaga tcctcacttc caccaaaggc
    20161 gacaaggaaa aagcggccca aacccttcag gtcagcctgg ccacgctcta tcggaaactt
    20221 tccgatgaag agccagcctc atcctaagaa tgcggatttt ttgcagtttt gcaaaaaaga
    20281 cctttcattt ttgagaaaag cggacggtca gccgttcccg gtttcgccta aatctgcgtg
    20341 tgtttctgat ttggcacgat gtctgctaaa ttcatttcgc taccgacaag gtgccggtcg
    20401 cgaagaaaac gagtgcacag tagtgaagga gcaaaccatg aaacaaaacc gcaaaggttt
    20461 cacgcttgtt gaaatcatga tcgtggtcgc gatcatcggc atcttggccg ctatcgctat
    20521 cccgaacttc atcaaatcgc gcaagcagtc acagatgaat gcctgtatcg cgaacctgaa
    20581 gcagattcaa
//
LOCUS       DAOSLE010000036        20253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2894_length_20253_cov_8.985543, whole genome shotgun sequence.
ACCESSION   DAOSLE010000036 DAOSLE010000000
VERSION     DAOSLE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 20253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20253
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_20253_cov_8.985543"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..866
                     /locus_tag="PK576_04125"
     CDS             318..866
                     /locus_tag="PK576_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60809.1"
                     /translation="MIQPREYAFCGLFGAAALLLPTLFHLLHLGHVFMPMYLPLVALA
                     FFVRPAASALTALLVPLLSGAVTGMPPFMPPVAPVMALELALMALMIGVLRQAVPRMP
                     VWCLLALALVVGRIVNASLLYVAARLLELPAGFVAGISLLSGWPGLLLMLAVIPGLVH
                     ATGHAQYHIHTPGASHDEPPVS"
     gene            844..1470
                     /locus_tag="PK576_04130"
     CDS             844..1470
                     /locus_tag="PK576_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE60810.1"
                     /translation="MTNPLCPDPRQPFFDSIADVWDDWHDLPALNARLADFFEHVDVQ
                     PGESVVDVGCGTGNLTLALLAWLGPQGRVTALDISPTMLARAARKTTDARVTWHEAPA
                     DRIPLPNGTCDRILCFSAWPHFDRLDHVVAEFRRVLCPGGQAHILHLISKEEVNRIHS
                     EAHPTVRADRLPPVDDVADLFRANGFTVIETEDNASRYLLSVRKEPEA"
     gene            1467..2219
                     /locus_tag="PK576_04135"
     CDS             1467..2219
                     /locus_tag="PK576_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOE60811.1"
                     /translation="MIAFSEAFGGGNGAARRLAPAVRLLCGAWVFVCCAVVPLGSPAG
                     IALFVVAWLAWLFGCAMPPRRLTLSLGFAICLFAPLLLLAPVAHWNGSAATWTEALRA
                     PLCVGVRGVAGVTIGVGALSLLPLTEFTDAVAALPLPRTVTSLLVQIVHQAALLTDET
                     RRMADAMRVRGVASARLAVRVRVVTAFPASWLTRLALRAARAGDAMEVRGFDGLPPNV
                     PPLTFSRSDWLALTVTTLLAVGALALRWRGCA"
     gene            2216..2932
                     /locus_tag="PK576_04140"
     CDS             2216..2932
                     /locus_tag="PK576_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60812.1"
                     /translation="MSAALAIHDLRVAYRAGDSEVLRGISLTLAPGEHCALLGLNGSG
                     KTTLLRAVAGLQPFSGTVTVCGIALRRETVRDVRDRIGFLFSTPDDQLLFPQVLADVS
                     FALERAGRPQAEAREAVLQTLEALGIAAYAERAPHTLSQGQRQRVALAGALVAQPPLL
                     LLDEPTASLDPVGRDELAQVLTRQPAALLLATHDLAFARQVARRFIMLEDGTITADTD
                     DPAHIEAAFAKHRLFLTQSH"
     gene            3139..4167
                     /locus_tag="PK576_04145"
     CDS             3139..4167
                     /locus_tag="PK576_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOE60813.1"
                     /translation="MKRNRINRNVGVLIAVVALAVLKAPAAGRLVVPSPAVVAMGEFP
                     DWEDRAARLPIQNAGDAPLTLKRVMLTCSCMRLDAFPKTLAPGERGEVVVTIQGKAVF
                     GGFDRVFYILSDDPQNPQAKVTLTGKTVPLFTVTCDVPTMLDKVDAGHVWTGRYTVAA
                     STPGVYLEAPRALNMGATCEFAIRTNRQEKMSYEVTHVVTFQEAERLESTLVFPVAGG
                     VTGGAQPPQGGYPPVRLRVVALHRQRVRALPDTLRITGAVKPVRRRILLRIEDEAALE
                     IAHLRCSSDVEGITLTPSKSVSGKGLYVDATIGAEALRTLKSKGGASLRFHYKQWPAA
                     EIKVLFSE"
     gene            4431..5471
                     /locus_tag="PK576_04150"
     CDS             4431..5471
                     /locus_tag="PK576_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60814.1"
                     /translation="MKKLLMFATAMTIAASTSFAITEVFDYKASVKHAYLKVVTARDS
                     SGVNQKVLSKYVKSASLKGYFILPCDGTPRATDNAGEAGKYGFLVLINNSAEKAVRDP
                     KIMPAKIDVDVWENFKNKSAKTTPVEGYFYAGGPFENSSVASTHLLFPLYTLLDYYYQ
                     SRFLFGLYNDFSLAYFHDAWMNHAGFGRATTSTTGGGCAVVSSHYSLDTLSGVVKGGL
                     YLCLKHGNLTTGSIFDSRPQGWEFYHIYDPYDAAWEDGYIRLQTTDVISGTWSLKRNT
                     RITPLALTTEEKAVLADIEVNRYIKRATDNLKRDYPAADPNESNWTQGVLPNGVVGDT
                     FAATYGINNPNP"
     gene            6148..11664
                     /locus_tag="PK576_04155"
     CDS             6148..11664
                     /locus_tag="PK576_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOE60815.1"
                     /translation="MRNKMLMSILAGALLLVFSGERASAQLVIHPTAAFGRWSSDLEG
                     ARQLAKANTNFYVICFARLSGCSSSDQANKVLFNSPGLYQWSISNKIALVYGDYNRGE
                     GDPLIAHIRRTYPDARGFPTIILVDGEGGSRVGLFGVSSGAVYNGMKLNPFNAQRFLE
                     VLDTFFEVRDDLPLKAMRLAASPTERSESLFRTYHGSQGKGNTLSASYSNVGFMYFQY
                     SDPVDWYCITNTVVQEGKGYRIAVSGAYDPSNLVSVSVYKNLTDANALTGAVASCSLM
                     ELAEMPLQYVPPLAGSHYIRFSREIVTNAIGKQEYDARYVLHYQQVDLSLGEYSFTTN
                     AVAYAEDLPAATVTVYRTNDLDKAATVRLLAGDGTAKRGIDYQVTNEVAFAANQATQQ
                     VAVALFNTGCVWRGDRTFRLKLDVNNPPTNIITELDFTILETDPDDDRYGPANHAVAG
                     APQFEFTSDRQSVTNLFLHHTNTVDHFVFTNLVAGQVYKIVVTNSCLTNAVNLRAELL
                     TNGVGFSTHVLAAQAGTVVEYTAATANDLTLRVARDANAPADPPACVCYAFAYYQWQK
                     PKYAFATNSITVESNAVVRLTVQRSEQTNEVTQVAWGTEDDTAKAGSDYVAATNDAVF
                     GIGEESQTIEVTVANMNDKYVGPRSFTVALQPGDKHDLGAVSNVTVTIAGDPVGGAQS
                     VWSNAVPLAVATTNISTGARYLNHTDTQDWIKLGPVESNKTYRVVVSDLLVKPAGYDA
                     GQAAVTFHYVAGGDIVVTNFPAMSLQSLSEQRVLLPFGGAMLAETNYVWVSVQRPADD
                     TVYLRYALDVREWVKPVVTFGYGAVDVPDTANHVQVAVLRTNNTEVSTSVDVWTEKGA
                     NTAAGTDYTHTSNRLVFAEGETNKTFTVPVTPDRSNVWTGDRQFGIVLRARDEVGDED
                     GIVYGPHTNVTVTLRENDPELDANDPADDAAAGAPVVPLSRVQTNLLATLNGSDTNDW
                     FRFSGILSNRLYRFGLTNFVHTGDAITEPVFSFFADEAGVVNGEALTNMPLSALLDEA
                     FIYRSLNTDDIWVRVARAPGAVHVRYALACRELMPCTARFAVTDVEVSEAAGQVRLQV
                     AVEVEEAGSLLDLDVFVRARTRDGTAVANQDYWPVSTVLEWKAGTAGGVQDVVIQLAN
                     YDYVWEGDESFYVDLEPESDVQVVAGAATATITLIEKDAPTRGQIAVAGTGDPMLDLK
                     AGGGVVAAREGDMVRVWFERSDGYAEDVTAIWTWTDGRDRTEVVLDGLFASREDGLKA
                     WDLVVPELPGYQAVRRITGTLSLRLPRGQTTPTLDRTRTTVTVQVTDRQFGGTLAEYA
                     ADPSHVPLRSGGDAWYVGVDGSIRSTPPIGANRPVVMAMTVKGPGTLRYTAEVANGDA
                     ACALTVCVGAGAAQAVAVGQNELAVPGGTQNVVWQFTRGMASPAEAHAVLRDIEWIPA
                     EDAEAYGTFAGYAYGREDRYGLASMTVAANGRIAGRLLFGHRIYAFTAAGYGEVMARA
                     GTERIPLTVAAVTDMEGRATGKIEIVEVSDEDDKMSAVLYRNGWGDRPMTEERARALE
                     ACVGYYTVALAEMYECGDGYLTITVAANGAVRAAGRLADGVAVSLGGTLALDADGVPH
                     VVLFSAPASYRGGYVWLYLKFDADAAGEAPTVIRGAEITGSEVPGCHWWSHNPRATGV
                     EGEGFLRFMDVGGGWYDRLATLRDYYAGRALGAEIDSAGQPFAPLYTGPSAARVTNEL
                     AAIATREWPVRLDVNAAGTGFIVTEQGKNAASRFTMRVNPVTGLFSGLLRAVYPYKGR
                     EITRSSVYQGVLTPVRPNPQDGLEGHGYFLMPDWSEDGTYQYKRSYPLWVTSGD"
     gene            11844..12995
                     /locus_tag="PK576_04160"
     CDS             11844..12995
                     /locus_tag="PK576_04160"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE60816.1"
                     /translation="MKAWVYLTLATFGLLAAGCGKQTGETQPEAAAEQGAATRPTDRV
                     EKMVKDMRPEDVIVEVQGVAFKKRDLDQRLLAIKARLDRDMNMVPQQKAVFLRQAMLS
                     SVPRFVDTQLLVADAKQKGVLDPSGIDATVSGKIDELVKKSKKPVATVYAQHPGGEMQ
                     LRRDIADGLWVEALIASNIPPKTVVDDAFIASVRVAIKADNDATTVTNNMKIAQLREI
                     RRAAMAGEVDFGEQADKISECELSAPGKKGYWGEFSRRDFDSVKLRTAVFALKAGEIC
                     DVHEDDEGYQIVRVDKRIAEQRNAQGVVIEPEAVVCSRIYLAKLPLLEIEADESLKKN
                     LQAQMRQQAISEYVEQLRTNGVYTVTYPHGTNFFGSAQTGRPDVANAHN"
     gene            13036..18489
                     /locus_tag="PK576_04165"
     CDS             13036..18489
                     /locus_tag="PK576_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOE60817.1"
                     /translation="MKSTVKHAINLAARPLRLALASALCACAVSTAWAQRNYATARAL
                     RVGSSSTVRLVDDGDGWGVYYFKVNMQMGRSYTFWTTGIDPEAELLVDDMTAEILYTG
                     WEDTDWVGEMNNERDGANMRLFVTQDDWDTEERLRYSYLVLYGDVGASVTVNSSAGIV
                     PEAIPPGDPENPAAFSVATTAPGLANGRLMDGSYWFRVSLTAGNKYKFATVGGTEDNE
                     LDLGITAVSPAEDGAGDHREEPVVSELLDPAWVDDFNPGILVVPQSTGLHFIEVTAME
                     DAAFFLKHQAVQARLPKDHPSTPLPIGGSLPCQPGYCNEPASGYFDQVIDQQLFKVTL
                     QRDGRYVFETTDLEGEAALVMRLYDAAGNILYSNTSKAPGDRNTLIAFQAPANGDYWV
                     GVSQDIENEDEEEPKPDVQCRVNARLLTAGEGLTDDWDPNDDVVAGANGLVVPPVAGP
                     EVDPLDVDEGHGPHTLGVNDWADIFGIAARRGVTYRLKTSLPEEADERVLSAQVFTLT
                     GAREMLVATIDDLSVGGDFTATAHGMYYVRVYLANGQGLDYGPYLLHACAYQQGAALG
                     ILQVNIMGPTAANGASWSLVSDGFAAPKYDGGMSVLLAAGAQTVRFASVAGWSTPANR
                     QVVVGAGNEPTVVTVKYNDTSDPRDDAPVGAVVINPTSREQTLSRSLWAEDPGDWFRM
                     SVRSGTYYNLRLELCSGAPWIRVYRTITAEAENVVAEGTECLFLARETATYYVQVGHQ
                     DPAFPTDSAYTLRHNSVTVGDVKLDRNAYRFREDAAKADFRVMRTGREGRVRVRYTTR
                     AGTAVPGINYMPVSGVLEWPAGNSAVQTVSVPLIPNLLPKWNGDTFFSIEIAPMPADE
                     LEGDEWVPSMAQPSTAVVTLTEVSAIVPGTLAFSGFGDVFSEPFENAWRPAVTVAAGD
                     PCTLWIERTVGSEGPVAVTVSTQRGSALDDQHFIGKTEQLFWADGDMTAKPFVLQTLQ
                     TGEAFMAAKNMTVRMAVDRTAGGAARLGTSSVAVTIQDPDLAQTVEAWSAGMGRTGPF
                     TVRAVPVGSWYFDRNGFCRSTPVAVRQRAEISFTLTGPGRLAFVPGLIKGGDDDNSTM
                     TATIGREVIPCENGDEIVRYLGRGSHVVRVTVMRGMTSPADAEVYGYLNPYGEQMPIL
                     WEPLAAATDPDPANPRGVVHLGGGAEGDALLQWRGDSEARYRVYVDLQRNKVGTPDAE
                     FVAVVDGLSFDTASVVNGGLAAGKTYFWRVDTVLADDEGNDLLVNTNTVWSFLTAAAD
                     AAATIVTGPDGTEIAGGTTLDLQQGLATEFQLGHTGGAAGFVSYMLAGGRLPDGLRLT
                     ANTGVISGTPTRAGEFSAVFQVRTGWVSGSTVTLNFRVLPIQLAAGTFNGLLETSDEV
                     PGIHYALASINMVVAENGRITARATVAGRPYTFAGTGYTEDIELLDNGQPGLRAVLTT
                     PVRSGFEWYTNTLTVTVCRGPDTDEEALDTIATAELVLNHVLPGNPVVQSTYFGDMVR
                     DNSRVPLVQPGIQDLAGYYTVSLPNEEASDGEPQGAGYLTITINANGTARLAGVLGDG
                     TAVSASVAPGYVSNYEEYLGGVQPALLLPVYVARQAMAIGGWLLIMREEDDPAGQPIV
                     TGELYWVNDDPNATRDGEEGYAWTVEAVGGFYNTLLNLQTYYLNQSLTVGDMTFDGLP
                     EQLDATYTKLLYWPGFDEAIPLTLVGSSFAQERRVLARDVNNVRLNDFEASVNPCSLN
                     VNLNRNTGVFQGNFGLWYGDDLGTLQREQAGIRIQGVMTPVKPSGSAYFDMPGMGLYQ
                     ISERIERRTWMGSYWFKIMAEDVEHDWSEGWDN"
     gene            18498..19376
                     /locus_tag="PK576_04170"
     CDS             18498..19376
                     /locus_tag="PK576_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE60818.1"
                     /translation="MKKSMMTPRLLIWLAVAGLCVLGLTLHIRQTDRRPAEKSSVEKA
                     GKTGRSHARKLRSSGKTGARSVADGTNENERVQSAAADAGQGQQEETNALPVVAKTQP
                     PLTPSQKKAQEMRSLLDDGDERAAMRQARELLTDDDPDVRAEVATVLGWIGLRALPEL
                     SQMLNDPEDHIADDVMVQWTMAYNEIEDPALQAEMLAAAMKVTKVNAYLEELAILSSQ
                     LPETYGVAAMVEVIEGPNPDAAAVAREHYEFMTQEPYSGPDAAAAWLRANGGDESDPQ
                     AAMQSETGEPNATPQN"
     gene            19410..20078
                     /locus_tag="PK576_04175"
     CDS             19410..20078
                     /locus_tag="PK576_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60819.1"
                     /translation="MKRFRWFAFAALMLIGCLIFYDRKREAVQVTDTRQEVGGPERKK
                     PVRSVAAQARTRGVGADKKRVRERPSSHASSVLPEKPSGGSQTNVTVSSVAEEQEQTA
                     RQTAVVAWENRVEALRDKTQAPSAQQAIAMKQAFDELHADDRLDAIQYALLLLPDMQI
                     SVIYPILLDKSQSEEVLDAIFDDVLNRDEQIKNPLMRELRKDKEHPLFFESARILDVV
                     EPES"
BASE COUNT         3910 a         5707 c         6661 g         3975 t
ORIGIN      
        1 gggctacccg atgccgggca tcatgtggta cactggcatg aaggtgaagt tctgatgcgg
       61 gcggagcccg caacccagtt caatctcaca caaaggcaca aaggacacaa agttttttca
      121 caagaggctc ttgcaaaacc ccttgattgc aggtgtggca cgggcatctt gcccgtgttt
      181 catgggcgag acgcccatgt cacgagggat tttgcaagcg cctcacaaat cctattccct
      241 tagtggcctt cgtgccttcg tgtgagaaac aatttgttcg atttcgatgg gacctggagc
      301 ctgagaccag aaaccaaatg attcagccta gggaatatgc cttttgcggc ctgttcggtg
      361 cggcggcgct gttgctgccg acgcttttcc atctgctaca tctggggcat gtcttcatgc
      421 cgatgtatct gccgctggtg gcgctggctt tttttgtgcg ccccgctgca tccgcgttga
      481 cagctctgct ggtgccgttg ctctccggcg cggtcaccgg catgccgccc ttcatgccgc
      541 cggtcgcccc ggtgatggcg ctggaactgg cactgatggc gctgatgatc ggcgtgttgc
      601 gccaagcggt gccacgcatg ccggtctggt gtctgctcgc gctcgcgttg gttgtcggac
      661 ggattgtgaa cgcatccttg ctctatgtcg ccgcgcgtct cttggaactg ccggccggtt
      721 tcgtggccgg catctcgctg ctcagcggct ggccgggtct gctgctgatg ttggcggtca
      781 tccccggcct cgtgcatgcc accggtcatg cgcagtacca tatccatacc cccggagcct
      841 cccatgacga accccctgtg tcctgatccg cgacaaccct ttttcgacag tatcgccgac
      901 gtctgggacg actggcacga cctgcccgcg ctcaacgcgc gactggccga tttcttcgaa
      961 cacgtcgatg tgcaaccggg tgagtcggtc gtagatgtcg gctgcggcac cggcaacctg
     1021 acgctcgccc tgctcgcgtg gctcggcccg caaggccggg tgaccgcgct cgacatttcg
     1081 ccgaccatgt tggcgcgcgc cgcgcgcaag acgaccgatg cgcgcgtgac gtggcacgag
     1141 gcaccggccg accgcatacc gctgccgaac gggacatgcg accgcatcct ctgcttttcc
     1201 gcctggcctc atttcgaccg tctggaccac gtagtcgccg agtttcgccg cgtgctgtgt
     1261 cccggcggcc aagcccacat cctgcatctg atctccaagg aagaggtcaa ccgcattcac
     1321 agcgaggccc atcccaccgt ccgcgccgac cgcctgccgc cggttgacga tgtggccgat
     1381 cttttccgag cgaacggttt cacagtgatc gagaccgaag acaacgcatc acgttacctg
     1441 ctgagcgtgc gcaaggagcc ggaggcatga tcgctttcag cgaagccttt ggcggcggca
     1501 atggcgcggc gcggcggctt gcccccgccg tgcgcctgtt gtgcggcgca tgggtcttcg
     1561 tctgctgcgc ggtcgtcccg ctcggttccc cggcgggcat cgcgcttttt gttgtcgcgt
     1621 ggctggcttg gctgttcggt tgcgctatgc cgccgcgacg gctcaccctg tcgctgggct
     1681 tcgccatctg cctcttcgcg ccgctgttgc tgctggcacc tgttgcgcac tggaacggct
     1741 ccgccgcaac gtggaccgaa gcgttgcggg caccgctatg tgtcggtgtg cgcggcgtgg
     1801 cgggcgtcac gataggcgtc ggtgcattgt cgctgctacc gctcaccgag ttcaccgacg
     1861 cggtggcggc cttgccgttg ccacgcaccg tgaccagtct gttggtgcag atcgtgcatc
     1921 aggccgcgtt gctgaccgac gaaacgcgac gcatggccga tgccatgcgg gtgcgcggcg
     1981 tagcctcggc gcgactcgcc gtgcgggtgc gcgtggtgac ggcgttcccg gccagttggt
     2041 tgacgcggct ggcgctgcgt gccgcacgcg cgggcgacgc gatggaggtg cgcggatttg
     2101 acggtctgcc gccgaatgtg ccgcccctga ctttttcccg ttctgactgg ctggcgctga
     2161 ccgtcaccac cctgctcgcc gtgggtgcgc tggcactgcg atggagagga tgcgcatgag
     2221 cgcggcactc gccatccatg atctgcgcgt cgcctaccgt gcgggcgact ccgaagttct
     2281 gcgcggcatc tccctgacgc tcgcacccgg cgaacactgc gccctgctgg ggctcaacgg
     2341 ctcgggcaag accacgttgc tgcgtgcggt cgcggggctg cagcccttca gcggaacggt
     2401 cacggtctgc ggtatcgcac tccggcgcga gacggtgcgc gacgtgcgcg accggatcgg
     2461 atttctcttc agtacgcccg atgaccaact cctgtttccg caggtgttgg ccgacgtgtc
     2521 gttcgctctg gaacgggccg gacgcccgca ggcagaagcg cgcgaagcgg tacttcagac
     2581 gttagaggcg ctgggcatcg cggcgtacgc cgagcgcgcg ccgcacacgc tgtcgcaggg
     2641 acagcgccag cgggtggcgc tggcgggagc actggtcgcg caaccaccgc tgctattgct
     2701 ggacgaaccc accgcctcgc tggatccggt cggacgcgat gaactcgcac aggtgctgac
     2761 gcgtcaaccg gcggcgctgc tgctcgccac gcacgacctc gccttcgcgc ggcaggtcgc
     2821 ccggcgcttc atcatgcttg aagacgggac gatcaccgct gacaccgatg atcctgcgca
     2881 catcgaagcg gccttcgcca aacaccggct tttcctcaca caaagccact aagggcacga
     2941 aggttttttc tctcacagac gcagagtgtg gcacgggcat cttgcccgtg tttcatgggc
     3001 gggacgccca tgccacaagc tttttttggg ggtgctccag ccgcaggtcg cgggtcgaac
     3061 cccctgtcac taaccgatta cccatatccc tcggttaagc ttgcggagat agcggcaaaa
     3121 cggcatgata ttccggcaat gaaaagaaac cggatcaatc ggaatgttgg tgttttaatt
     3181 gcagtcgtcg cgctggcggt tctcaaagcc ccggctgcgg gccgtctggt tgtgccgtcg
     3241 ccggcggtgg tggcgatggg cgagttccca gattgggagg atcgcgcggc ccgcctgccg
     3301 atccaaaacg cgggggacgc gccgctgacg ctgaagcgtg tcatgttgac ctgttcctgt
     3361 atgcgtttgg acgcctttcc caagaccctg gctcccggcg agcggggtga ggttgtggtc
     3421 acgattcagg gcaaggccgt gtttggaggc tttgatcgcg tcttctatat tctcagcgac
     3481 gacccgcaga acccgcaggc caaagtgacg ctcaccggca agaccgtgcc gctcttcacc
     3541 gtgacgtgcg acgtgcccac catgctggac aaggttgacg cgggacatgt ctggaccgga
     3601 cggtatacgg tcgcggccag cacccccggg gtttatctag aggctccccg ggcgctcaac
     3661 atgggcgcga cctgcgagtt cgcgatccgt accaaccgcc aagagaagat gagttacgag
     3721 gtcacgcacg tggtcacttt ccaagaggcg gaacggcttg agagcacgct ggtctttccg
     3781 gtggcggggg gcgtcacagg gggagcccaa ccgccgcagg gggggtaccc gccggtgcgc
     3841 ttgagggtcg tcgcgctaca ccggcagcgg gtccgtgcgc tgccggacac attgcgtatc
     3901 accggcgccg taaagccggt gcgcagacgc atcttgctgc ggatcgagga cgaggccgcg
     3961 ctggagatcg cgcacctgcg gtgctcttcc gatgtcgagg gcatcaccct gacgccaagt
     4021 aaaagtgtca gcggaaaagg cctgtacgtg gatgcgacga tcggcgccga ggcgttgcgg
     4081 acgctcaaga gcaagggtgg cgcgagcctg cgtttccact acaagcagtg gccagccgca
     4141 gaaatcaagg tgcttttcag cgagtgagga agcaatcggt tgctaaccgg tctctaaaaa
     4201 aaagtaaaaa aagtgatttt tgctgttgac aggttgcgcg tggtttaata aagtcgctcc
     4261 gttttcagac ctcaaaaggt tctcggacac ggtgtccgac cctaggccaa cggcgccgat
     4321 ggatgttaac ccgacgaggc tctgagtgtt acagtaacaa ggctgtgagg gagtcgggcg
     4381 acgggtgtcc ctgaccgtca cagcttaaac ggaatgagag gtcatactct atgaagaagt
     4441 tgttaatgtt tgctacggcg atgacgattg ccgcgagcac atcatttgct atcacggagg
     4501 tctttgatta taaggccagc gtgaagcatg cttacctgaa ggtggttact gccagagatt
     4561 caagcggcgt taaccagaag gttctgtcca agtatgtgaa gtccgcgtcg ctcaagggct
     4621 attttattct gccttgcgac ggtactccga gagcgacaga caacgccggt gaagctggca
     4681 agtatgggtt tctggtcctg ataaacaaca gcgcggaaaa agctgtccgt gacccgaaaa
     4741 tcatgccggc caagatcgac gtggacgtat gggagaactt caaaaacaaa agcgcgaaga
     4801 ccactccggt cgaggggtac ttctacgcgg gcgggccttt tgagaattcg tctgtggcct
     4861 cgacgcattt gctcttcccg ctctacaccc ttctggacta ctattaccag agccgcttcc
     4921 tgttcggtct ctacaatgac ttctccttgg cgtacttcca cgatgcgtgg atgaaccatg
     4981 ccggtttcgg aagggcgacc acttccacaa ccggcggggg gtgcgctgtg gtgagttcac
     5041 attattccct cgacaccttg tcgggtgtgg tcaaaggcgg actgtacctg tgcctgaaac
     5101 acggcaatct gacgacaggt tctatattcg atagtcgccc gcaagggtgg gagttctacc
     5161 acatttacga tccttatgat gccgcgtggg aggatgggta tatccgtctg caaaccacgg
     5221 atgtcatcag cggtacttgg tcgctcaagc gcaacaccag gatcaccccg cttgccctga
     5281 ccaccgagga gaaggcggtt ctggctgaca tcgaggtcaa ccgttacatc aagcgtgcga
     5341 ccgataacct gaagagggac tatccggctg ctgaccccaa tgagtcgaac tggactcagg
     5401 gcgtgcttcc gaatggagtg gttggcgata ccttcgcggc gacgtacggg ataaataatc
     5461 ctaacccgta atcggagaag ggttcctgac agtcgattca ggctgtaaaa gaacggaatc
     5521 aaacgggacg gcgttcgcgc cgtcccgttt ttttattgtg ttggcgcgat cggttggtga
     5581 cagaggtcgc ccacacttag tcggtcacac ttagtcgccc acacttagtc ggtcgaacaa
     5641 gcgacgaagt gtatcgacta ggcgtaagcg actaagtgta tgggactaag tgtatgggac
     5701 gaagtgtatg cgacgctcaa cgcttaaaaa aactcctaac tcctaaatgt taactccgcg
     5761 cgtagcgcgg cggactgtcg ccctccaagc ttcgcgcctt ggcggttcca aaaaaccgag
     5821 ccctgctttt actctcacgg ttgcgagcca ctgtgtggtc gcaacaagag aggttgaccc
     5881 gcgacattcg acccgcgact tgcggctggc cctgcccccc caaaaacctt cgtgtccttc
     5941 gtgccttcgt gtgagaaaaa accgagcaat gccatccccc ccaaacctct gcgtctctgc
     6001 gtctctgcgt taaataaacc ccgcgccctg cccgttacgc ccaaaaaatt acaaattgcg
     6061 acttgcgcgg accacccctt atctgctacc atcttaattt ctatctgaca gcactctttc
     6121 accatcctca tcttcaaggg tttccgtatg cgtaataaga tgttaatgag tattttggca
     6181 ggcgccctgc ttcttgtttt ttcaggcgag agggcctcgg cgcagttggt gatccatccc
     6241 acggcagcct tcgggcgctg gtcgagtgat ttggaagggg cgcgccaact ggcgaaggcg
     6301 aacaccaatt tctatgtgat ctgttttgct cgtttgagcg ggtgctcaag ttccgatcag
     6361 gccaacaagg tgctttttaa ttcgccgggg ctttatcaat ggtcgatttc aaataaaatc
     6421 gccttggtgt acggagatta caaccggggc gagggtgatc ccctgattgc ccacattcgc
     6481 agaacctatc cggatgccag gggctttcca accatcattc ttgtggacgg ggagggcggg
     6541 tctcgggttg gtctttttgg tgtgtcatcg ggtgcggttt acaacgggat gaaattaaac
     6601 ccgtttaatg cacagcgttt cctcgaagtc ttagatacgt tttttgaggt ccgcgacgat
     6661 cttccgctca aggcgatgcg gcttgccgca tcgcccaccg aacgctccga gagtcttttc
     6721 aggacatatc acggctcgca gggcaaggga aacacgctga gcgcctcgta ctcgaatgtg
     6781 gggttcatgt atttccagta ttcggatccc gtggattggt attgcatcac caacacagtg
     6841 gttcaggagg gcaagggcta ccggatcgcg gtctccggcg cgtatgatcc cagcaatctg
     6901 gtctcggtct cggtctacaa aaacttgacg gacgcgaacg ccttgacggg cgcggtcgcg
     6961 agctgctcgc tgatggagct ggcggaaatg ccgctgcagt acgtgccgcc tctggcgggt
     7021 agccactaca tccgcttcag ccgcgagatc gtcaccaacg ccatcggcaa acaggagtac
     7081 gacgcccgtt atgtcctgca ttaccagcag gtggacctct cgctcgggga atattcattc
     7141 acgaccaacg cggtggcgta tgctgaggat ctcccggcgg cgacagtcac cgtctaccgc
     7201 accaacgatc tcgataaagc cgccacggtg cggctgttgg cgggggacgg cacggccaag
     7261 cgggggatcg actatcaggt gaccaacgag gtcgcgttcg cggcgaacca ggccacgcag
     7321 caggtggcgg tggccctctt caacacaggg tgcgtctggc gcggcgaccg cacgttcagg
     7381 ctgaaactgg acgtgaacaa tccgcccacg aatataatta cggaactgga cttcaccatt
     7441 ctggaaaccg accccgacga cgaccggtac ggccccgcca accatgcggt ggcaggcgcg
     7501 ccgcagttcg aatttacctc tgaccggcaa tccgtgacca atctcttcct gcaccacacc
     7561 aatacggtcg accacttcgt gttcacgaat ctggtggcgg gtcaagttta caagatcgtg
     7621 gtcaccaatt cctgcctgac caacgcggtc aacctgcggg ccgagttgct gaccaacggc
     7681 gtaggttttt ctacccatgt tctggccgcg caagccggga ccgtggtcga atacacagcg
     7741 gccaccgcga acgacctgac gctccgcgtt gcacgcgatg ccaacgctcc tgccgatccc
     7801 ccggcctgcg tctgttacgc attcgcttat taccaatggc aaaagccgaa gtacgccttt
     7861 gcgacaaact caatcacggt tgaatcgaat gcggtagtta ggctgaccgt tcagcgatct
     7921 gagcaaacca acgaggtgac acaggtcgcg tggggcacag aggacgatac ggccaaggct
     7981 ggatcggatt atgtggcggc cacgaacgac gcggtttttg gaatcggaga ggagtcgcag
     8041 acgatcgagg tcaccgtcgc caacatgaac gacaagtatg tggggccacg ctcgttcacc
     8101 gttgcgctgc agccgggaga caagcatgat ctcggcgcgg tgtcgaacgt caccgtgacc
     8161 atcgcgggcg atcccgttgg cggggcgcaa tccgtatgga gcaacgccgt gccgctggct
     8221 gtcgctacga ccaacatttc aaccggtgcc cgctatctga accacaccga cacccaggac
     8281 tggatcaaac tcgggccggt ggagtccaac aagacctacc gggtcgtggt ctccgacttg
     8341 ctggtcaagc cagcgggtta tgacgcaggg caggccgcag tcaccttcca ttatgtcgcg
     8401 ggcggcgaca tcgttgtaac gaactttcct gcgatgtccc tgcaaagcct gtccgaacag
     8461 agagtgttgc tgccgttcgg cggagcgatg ctggcggaaa ccaactacgt ctgggtcagc
     8521 gtccagcgtc ctgcggatga cacggtctat ctgcgctatg cgctggacgt gcgcgagtgg
     8581 gtcaagccgg tggtgacctt cgggtatggc gcggtggacg tgcctgacac ggccaaccac
     8641 gtgcaggtgg cggtcctgcg gaccaacaac accgaggtga gcacctctgt tgatgtctgg
     8701 accgagaagg gcgcaaacac ggcggcgggc acggattata cccatacctc gaaccggttg
     8761 gtgttcgcgg agggcgagac caacaagacg ttcaccgtgc cggtgacgcc cgaccggtcg
     8821 aacgtgtgga ccggcgaccg ccagttcggg atcgtcttgc gtgccaggga cgaagtcggc
     8881 gacgaagacg ggatcgttta cggtccgcac accaatgtga cggtgaccct tcgcgagaat
     8941 gatccggaat tggacgcgaa cgatccggcg gatgacgctg cggctggcgc accggtcgtc
     9001 ccgttgtcgc gcgtgcagac caacctgctg gccacgctga acggatcgga caccaacgac
     9061 tggttccggt tcagcgggat tctctccaac cggctctacc ggttcgggct aaccaacttc
     9121 gtgcataccg gggatgcgat aaccgagccg gtgttctcgt tctttgcgga cgaagcgggc
     9181 gtcgtgaacg gcgaggcctt gacgaacatg ccgttgtccg cgttgcttga tgaggcattc
     9241 atctaccgtt cgctcaatac ggatgatatt tgggtgcgcg tggcgcgcgc ccccggggcg
     9301 gtgcatgtgc gctacgcgct ggcctgccgc gagctgatgc cgtgtacggc ccgtttcgcg
     9361 gtcacggacg tggaggtcag cgaggcggcc ggccaggtgc ggctgcaagt ggcggtggaa
     9421 gttgaggagg cgggttcgct gctggacttg gatgtgttcg tgcgggcacg tacccgggac
     9481 ggcacggcgg tggccaatca ggactactgg ccggtttcca cggttctgga gtggaaggcg
     9541 ggaacggccg gcggcgtgca ggatgtggtg atccagttgg cgaactatga ttatgtctgg
     9601 gagggtgacg agagcttcta tgtggacctc gagccggagt cggacgtgca ggtggttgcg
     9661 ggcgcggcga ccgcgacgat cacgttgatt gagaaagacg cgccgacgcg cggccagatc
     9721 gcggttgcgg gcacgggcga cccgatgctt gacctgaaag cgggcggcgg tgttgtggcc
     9781 gcgcgcgaag gcgacatggt gcgcgtctgg ttcgagcgta gcgatggata tgccgaggat
     9841 gtgacggcga tctggacctg gaccgacgga agagaccgca cggaggtggt gctcgacggg
     9901 ctcttcgcca gccgcgagga cgggttgaag gcatgggatc tggtggtgcc ggagctgccg
     9961 ggctaccaag ccgtgcgccg gatcacgggg acattgtcgt tgcggttgcc gcgcgggcag
    10021 acgacgccca cgcttgaccg cacccgcacg accgtgaccg tgcaggtcac ggaccgccag
    10081 tttggcggga cgcttgccga gtatgcggcg gacccgtctc atgtgccgct gcgttccggc
    10141 ggggacgcgt ggtatgtggg tgttgacggg tcgatccgca gcacgccgcc gatcggagcg
    10201 aaccgtcccg tggtgatggc gatgacggtc aaaggccccg gtacgttgcg gtacacggct
    10261 gaggtggcga acggcgatgc cgcctgcgcc ttgacggtgt gtgtcggtgc gggcgcggcg
    10321 caagcggtgg ctgtcggcca gaacgaactg gcggtgccgg gcggcacgca gaacgtggtc
    10381 tggcagttca cgcgcggcat ggcctcgcct gcggaggcgc atgcggtgct gcgggacatc
    10441 gagtggatcc cggcggaaga cgcggaggcc tacggcacct ttgccgggta cgcatacggt
    10501 agggaagacc ggtacgggtt ggcgagcatg accgtggcgg ccaacgggcg aatcgccggg
    10561 cggttgctgt tcggtcaccg gatctatgcg ttcacggcag ccggttacgg cgaggtgatg
    10621 gcccgggccg gaaccgaaag gatcccgctg actgtcgcag ccgtgacaga catggaaggc
    10681 cgcgccacgg ggaagatcga gatcgttgag gtttcggacg aagacgataa gatgtcggcg
    10741 gtgttgtacc gcaacggctg gggtgaccgg cccatgacgg aggagcgggc gcgggcgctg
    10801 gaagcctgcg tgggatacta cacggtcgcg ctcgccgaaa tgtacgagtg cggcgacggc
    10861 tacctgacga tcaccgtggc ggcgaacggg gcggtcaggg ctgccggacg gctggcggac
    10921 ggggtcgcgg tttctctggg cgggacgctg gcgctggatg cggacggcgt gccgcatgtg
    10981 gtgctgtttt cggctccggc gtcgtaccgc ggcggctatg tctggctcta cctcaagttt
    11041 gacgcggatg cggcggggga ggcgccgacg gtgatccggg gcgcggagat tacgggttcg
    11101 gaggttcccg ggtgtcactg gtggagtcac aacccccgcg ccaccggtgt ggagggcgag
    11161 gggttcctgc gtttcatgga tgtgggcggg ggctggtatg accgtctggc caccctgcgg
    11221 gattactatg cggggagggc tctgggcgcc gagatcgatt ccgccgggca acccttcgcg
    11281 ccgttgtaca cggggccgtc cgccgccagg gtgacgaacg agctggccgc gatcgcgacc
    11341 cgcgaatggc cggtgcgctt ggatgtcaac gcggcgggaa ccggttttat cgtgacggag
    11401 cagggcaaga acgccgcttc gcgcttcacc atgcgggtca atccggtgac gggactgttt
    11461 tcggggctgc tgagggcggt ctacccctat aaaggacgtg aaatcacccg aagcagcgtg
    11521 tatcagggtg ttttgacacc ggtacggccc aacccgcagg acggtctgga agggcacggg
    11581 tattttctga tgcctgactg gtcggaagac gggacctatc agtacaaacg gtcttacccg
    11641 ttgtgggtta cctcgggcga ttgagaggct tgacgcgctc atccatgaag agaaagcaca
    11701 tgttttttgg gacagatatg agtgtgatgg ctcttgcaaa accctttgct tgcaggtgtg
    11761 gcacgggcat cttgcccgtg ttccatgggc gggacgccca tgccacgggg ggtagcacgg
    11821 gcatcttgcc cgtgtttaaa acaatgaaag cgtgggtgta tctaacgctg gcgaccttcg
    11881 gcctgcttgc cgcaggatgc ggcaagcaga ctggagaaac gcagcctgaa gccgccgcag
    11941 agcagggtgc cgcgacccgc ccgacggaca gggtcgagaa gatggtcaag gatatgcgac
    12001 ctgaggatgt gatcgtcgag gttcaaggcg tggcattcaa gaaaagggat cttgaccaac
    12061 gtttgctagc cataaaagcc agactggatc gtgatatgaa catggtgcca cagcaaaaag
    12121 ccgtgtttct gcgacaagcg atgctatcga gcgtgccgag gtttgtggat acccaactgc
    12181 tggttgcgga cgccaagcaa aagggggtgt tggatccgtc gggaattgac gcgaccgtgt
    12241 cggggaaaat agacgagtta gtgaaaaaga gcaaaaagcc ggttgcgacc gtgtacgcgc
    12301 agcacccggg gggggagatg cagctcaggc gcgatatcgc ggacggtctt tgggtcgaag
    12361 ccctgatcgc ctccaacatt ccgccgaaaa cggttgtgga cgatgctttt atcgcgtcgg
    12421 tgcgtgtagc cataaaggcg gacaacgacg cgaccacagt tacgaacaac atgaagatcg
    12481 cgcaattgcg cgagatccgg cgtgcggcaa tggctggaga ggtggatttc ggcgaacagg
    12541 cggataaaat ctcggagtgc gagttatcgg ctccgggcaa gaaggggtac tggggcgagt
    12601 tctcccggag agattttgat agcgtgaagt tgcgcacagc ggttttcgcg ttgaaggcgg
    12661 gggagatctg cgacgtgcat gaggacgacg agggctacca gatcgtgcgg gtcgacaagc
    12721 gtatcgcgga gcagcgtaac gcccaagggg ttgtgattga accggaggcg gtcgtgtgct
    12781 cgcggatcta tctggccaag ttacccttgc tggagatcga ggcggacgaa agcttgaaga
    12841 agaacctgca ggcgcagatg cggcaacagg cgatcagcga atatgtcgag caattgcgga
    12901 cgaacggcgt ctacacggtg acgtatccgc acgggacaaa tttcttcggg agtgcgcaaa
    12961 caggcaggcc agatgtggcc aacgcgcaca actaaaaccc tcaacggaca aacgggaaag
    13021 cgataggagt caacgatgaa atcgacggta aaacatgcaa tcaatctggc ggcaaggccc
    13081 ctgcgtctgg cactggcgtc tgccctttgc gcctgtgcgg tctcgaccgc gtgggcgcag
    13141 cgcaactacg cgaccgcgag agcgctcagg gtaggcagct cgtcgaccgt gcgtctggtc
    13201 gatgacggtg atggctgggg cgtctattat ttcaaagtga acatgcaaat gggccggtcc
    13261 tataccttct ggactacagg catcgatccg gaagcggagt tgctggtcga cgacatgacc
    13321 gccgaaatcc tgtacacggg ttgggaggac acggattggg tgggtgagat gaacaacgag
    13381 cgggacgggg ccaacatgcg cctgttcgtg acccaggacg actgggatac cgaagagcgg
    13441 ctcaggtaca gctatttggt tctatacgga gatgtcgggg cctcggtcac ggttaactcc
    13501 tctgccggga tcgtgccgga agctatcccg ccaggcgatc ctgaaaatcc cgccgccttc
    13561 tctgtggcca cgacagcgcc cggcctggcg aacggccgcc ttatggatgg atcctattgg
    13621 tttcgggtgt cattgacagc ggggaataaa tacaagtttg ccacggtcgg cggcacggag
    13681 gacaacgagc tggatctggg gattacggct gtgtctcccg cagaggatgg ggcgggcgac
    13741 caccgggagg aaccggtggt gagcgagttg ttggatccgg catgggtgga tgatttcaac
    13801 cccggcattc tggtggtgcc gcaaagcacc gggttgcatt tcatcgaagt cacggcgatg
    13861 gaggacgccg cgttcttcct gaaacatcag gctgtgcagg cgcggctgcc caaggaccat
    13921 ccctcgacac cgttgccgat cggtggcagt ctgccgtgcc agccggggta ctgcaacgag
    13981 cctgccagcg gctatttcga ccaggtgatc gaccagcagc tcttcaaggt gacgctgcag
    14041 agggacgggc gctatgtgtt cgagacgacg gatctggagg gcgaggcagc tctggtcatg
    14101 cggctgtatg acgctgccgg caatatcctc tacagcaata cctccaaagc gcccggcgac
    14161 cggaacacgt tgatcgcgtt tcaggctccg gcgaacggcg attactgggt cggcgtcagt
    14221 caggacattg agaacgagga tgaggaagaa ccgaagccgg atgtgcagtg cagggtcaat
    14281 gcccggctgc tgaccgcggg tgaagggtta accgacgact gggacccgaa cgacgatgtc
    14341 gtggccggtg ccaacggtct ggtggttccg ccggttgcgg ggccggaggt cgatccttta
    14401 gatgttgatg aagggcacgg gccgcacacg cttggcgtga acgactgggc agacattttc
    14461 ggcatcgcgg cgcgcagggg cgtgacgtat cggctcaaaa catcgttgcc ggaagaggcc
    14521 gacgagcgtg tcctttccgc gcaggtgttc actttgaccg gtgcccgcga gatgttggtg
    14581 gccacgattg acgatctctc cgtaggcggc gatttcaccg ccacggctca cggtatgtat
    14641 tatgtgcggg tctatctggc taacgggcaa gggctggact acgggccgta cctgctgcac
    14701 gcgtgtgcat atcagcaagg cgcggcgttg ggtattctgc aggtcaacat catggggccc
    14761 acggctgcta acggcgcctc gtggtcgctg gtgagcgacg ggttcgcggc acccaaatat
    14821 gacggcggta tgagcgtgct gctggcggct ggcgcccaga ccgtgcgctt tgcctcggtt
    14881 gccggttggt caacccccgc taaccggcaa gtcgttgtcg gtgccgggaa tgagccgacg
    14941 gtcgtcacgg tcaaatacaa cgacacctcc gatccgcggg atgacgcgcc ggtcggcgcg
    15001 gtagtcatca acccgacatc acgcgagcaa acgctttcgc gcagcttgtg ggccgaggat
    15061 ccgggcgatt ggttccgcat gtccgtgcgt tccggaacct attacaatct gagactggag
    15121 ctttgcagcg gtgcgccgtg gatacgcgtc taccgcacga tcacggctga agccgaaaac
    15181 gtggtggcgg aaggaaccga atgcctgttt ctggcgcgcg aaaccgcgac ctattatgtg
    15241 caggtcggcc atcaggatcc ggcgttcccg acagacagcg cgtacacgct gaggcacaat
    15301 tcggtgacgg tgggcgatgt caaactggac cggaacgcct accgtttccg cgaagacgcc
    15361 gccaaggcgg acttccgtgt gatgcggacc ggacgcgagg gccgtgtgcg cgtccgctac
    15421 acgacccgcg ccggcacggc tgtgccggga atcaattaca tgccggtctc gggcgttttg
    15481 gagtggcctg ccggcaactc agcggttcag acggtcagcg ttccgctgat ccccaatctc
    15541 ctgcccaaat ggaacggaga caccttcttc tccatcgaaa tcgcgccgat gcctgcggac
    15601 gaacttgaag gcgacgagtg ggtgccctca atggcgcaac cttcgacggc ggtcgtgacg
    15661 ctgacggaag tgagtgccat agtgccggga acgctcgcgt tctcggggtt cggcgatgtt
    15721 ttcagcgagc cgttcgaaaa cgcatggcgg ccggcggtca cggtagcggc cggtgatcca
    15781 tgcacgctct ggatcgagcg gaccgtgggt tcggagggtc ctgtggcggt cacggtttcc
    15841 acgcaacgcg gctctgcgct tgatgatcag cacttcatcg ggaagacaga gcagttgttc
    15901 tgggccgacg gagacatgac ggccaagcct ttcgtgctgc agacgctgca gaccggcgag
    15961 gcgttcatgg cagccaaaaa catgacggtc cggatggccg tcgacagaac ggcggggggg
    16021 gcggcacgtc tcggcacctc ttcggtggcg gtcacgattc aggatcctga tctcgcgcag
    16081 acggtcgagg cgtggtcggc tggcatgggg cgcaccggcc cgttcacggt gcgggcggtt
    16141 ccggtcggct cctggtactt tgaccggaac ggattctgcc gctccacgcc cgtcgcggtg
    16201 cggcaacggg cggagatctc ctttacgctg acggggccgg gcaggctggc tttcgtaccc
    16261 gggctgatca aggggggcga cgacgacaac agcacgatga ctgcgacgat cggccgggag
    16321 gttatacctt gcgagaacgg cgacgagatc gtgaggtatc tcggcagggg gtcgcatgtt
    16381 gtgcgcgtga cagtcatgcg cggcatgacc tcgcctgcgg acgcggaggt gtacggctat
    16441 ctgaatccgt atggcgagca gatgccgata ctttgggagc ctttggctgc cgccaccgat
    16501 cccgatccgg ccaatcccag aggcgttgtc catctgggcg gcggagcgga aggggacgca
    16561 ttgctgcaat ggcgcggtga cagcgaggct cgttatcgcg tttatgtgga cctgcagcgc
    16621 aataaagtcg gcacgccgga tgcggagttc gtggcggttg tggacggatt gtcgttcgat
    16681 accgcttcgg tcgtgaacgg ggggctcgct gccggcaaga cctacttctg gcgcgtagac
    16741 acggtcttgg ccgacgatga ggggaatgac ctgctggtca acacgaacac ggtatggagt
    16801 ttcctgacag ccgccgctga tgcggccgcg accatcgtca ccggtcctga cggaacggag
    16861 atcgcaggcg gaaccaccct tgatttgcag caagggttgg cgacggagtt ccaactgggg
    16921 cacaccggcg gggccgccgg cttcgtgagc tacatgctgg ctggcgggcg tctgccggac
    16981 gggttgcggc tgacagccaa cacgggggtg atctcgggca cgccgacccg cgcgggagag
    17041 ttcagcgcgg tgttccaagt tcggacgggt tgggtctccg gcagcacggt gacgctcaat
    17101 ttccgtgtgt tgccgatcca actcgcagcc ggaacattta acggcttgct ggaaacatcc
    17161 gatgaggtcc ccggcattca ttatgcgctg gcttcgatca atatggttgt ggcggaaaac
    17221 gggcgcatca ccgcgcgcgc gacagtggcg ggccgtccgt atacgtttgc cggaacagga
    17281 tacacggaag atatcgaact gctcgacaat gggcagcccg gattgcgcgc ggtgttgacc
    17341 acacctgtcc gctccggttt cgagtggtat accaacacct tgacggtcac ggtgtgcagg
    17401 ggaccggata ccgacgaaga ggcgctggat acgatcgcga cggcggaact cgtgctcaac
    17461 catgtgctgc cgggcaaccc ggtggtgcag tcaacgtact tcggcgatat ggtccgcgac
    17521 aacagcaggg ttccgctggt gcagccgggg atccaagatc tggccggata ctacacggtc
    17581 tcgctgccga acgaagaagc gtcagacgga gaaccgcagg gagccgggta tctgaccatc
    17641 acgatcaacg ccaacggcac ggcgcgcctt gcgggtgtgc tcggcgacgg cactgctgtc
    17701 tcggcatctg tcgcgccagg gtatgtgtcg aactatgaag agtatcttgg aggcgtgcag
    17761 cccgctctgt tgctccccgt ctacgttgct agacaggcaa tggcaatcgg cggctggctc
    17821 ttgatcatgc gtgaagagga tgatcctgcg ggacagccga ttgtgacggg tgaactttac
    17881 tgggtgaacg atgatccgaa tgccacccgt gacggtgaag agggatatgc gtggactgtg
    17941 gaggcggtgg gagggttcta taatacgctc ctgaacctac agacgtatta cctgaaccag
    18001 agtttgacag tcggcgatat gacctttgac gggttgccgg aacagttgga tgcgacctac
    18061 acgaaactgc tctattggcc cggctttgac gaggcgattc cgctgactct ggtcggcagc
    18121 tcattcgcgc aggaacggcg cgtgttggcc agagatgtaa acaatgtcag gttgaacgat
    18181 ttcgaggcat ccgtcaatcc atgcagcctg aacgtgaacc tgaatcggaa tacgggtgtt
    18241 tttcagggca acttcggact ctggtacggc gatgatctcg gcacgttgca aagggaacag
    18301 gccggcatca ggatccaagg cgtgatgact ccggtcaagc cgagtggcag cgcctatttt
    18361 gacatgccgg gcatggggtt gtaccagatc agcgaacgga tcgaaaggcg cacctggatg
    18421 ggttcgtatt ggttcaagat tatggcggaa gatgtcgagc acgactggtc ggaaggctgg
    18481 gacaactagg attcggcatg aagaaaagca tgatgacgcc ccgcttgctg atatggctgg
    18541 cggttgccgg attgtgtgtg ctggggctca ctctgcatat ccggcagacc gataggcgtc
    18601 ctgcagaaaa atcttctgta gagaaagcgg ggaaaacggg gcggagtcat gccagaaaac
    18661 tgcgatcctc cgggaaaacc ggggcgcggt ccgttgcgga cggcacgaat gaaaatgaac
    18721 gtgtgcaatc tgcggcggcg gacgcgggtc aaggtcagca ggaggagacc aatgcactac
    18781 cggttgtcgc gaagacgcag cctccgctga cacccagcca gaaaaaggcg caggagatgc
    18841 ggagtctgct agatgacggc gatgagcgtg cggcgatgcg tcaggccaga gagctgctca
    18901 cggacgatga tccggatgtt cgtgccgaag tggcgaccgt tttaggctgg atcgggttgc
    18961 gtgctttgcc ggagttgtca cagatgttga atgacccgga agatcatatc gcagacgatg
    19021 ttatggtcca gtggaccatg gcatataacg aaattgagga tccggctttg caggctgaaa
    19081 tgttggctgc ggcgatgaag gtcaccaagg tgaacgcata tttggaggag ttggccatcc
    19141 tctcttctca actgcctgag acgtatggcg tggctgcgat ggtagaggtc atcgaggggc
    19201 cgaaccctga cgccgccgca gtggcgcggg agcactacga atttatgact caggaaccct
    19261 actctggacc tgacgctgcc gcagcgtggt tgcgcgcaaa tggaggggat gagtccgacc
    19321 ctcaggcagc catgcaatcc gaaaccgggg agccgaacgc cacgccacag aactagcgcg
    19381 ggcgcagccc gcaacccaat tcaattctca tgaaacggtt cagatggttt gcttttgcgg
    19441 ccttgatgtt gattggttgc ttgatttttt acgaccgcaa gcgtgaggcg gtacaggtca
    19501 ccgataccag gcaagaagta ggggggccgg aacgtaaaaa gcctgtccgc agcgttgccg
    19561 cgcaagctcg aacgcgcggg gtcggagccg ataaaaagcg cgtgcgtgaa cgcccgtcgt
    19621 cacacgcgtc ttccgttttg ccggaaaagc cgtcaggcgg ttcgcagacg aacgtcacgg
    19681 tttccagcgt tgccgaggag caggagcaga ccgcgcgcca gaccgcagtg gtggcttggg
    19741 agaatcgggt tgaggcgctg agggataaga cacaagcccc gtctgctcag caggctattg
    19801 cgatgaaaca agcctttgac gagctgcatg ccgatgaccg tctggatgcc atccaatacg
    19861 cgctcttgtt gttgcctgac atgcaaatca gcgtgatcta cccgatcttg ctcgataaaa
    19921 gtcagtctga agaagttttg gacgcgattt tcgatgatgt tctgaaccgc gatgagcaga
    19981 tcaaaaatcc actgatgcgc gaattgcgca aggataaaga acatccgctc ttttttgaat
    20041 ctgcacgcat cttggatgtg gttgaacccg aatcctgaaa ccatcctctg tgcgcaacgc
    20101 ggagatgact ttacacgccc gcccctctca ggtatcatcg cgccatgttt acagcacaca
    20161 cacccgaatt gatggacgat ttgcgactgc ggtggtcgcg catgcaggaa gcactgcgca
    20221 ccgatggcgc agatgcgctg ctggtcgcca cca
//
LOCUS       DAOSLE010000037        19901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2967_length_19901_cov_7.334224, whole genome shotgun sequence.
ACCESSION   DAOSLE010000037 DAOSLE010000000
VERSION     DAOSLE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19901
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2967_length_19901_cov_7.334224"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(371..1021)
                     /locus_tag="PK576_04180"
     CDS             complement(371..1021)
                     /locus_tag="PK576_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE60820.1"
                     /translation="MKTNDPVSETASAAIRPTIFLVDDDPSVRKALPRLLQAAGYEVI
                     TFASAQEFLDRPRFDGDGCLVLDVRMTGMDGLALQEELAKDDSPLPIVFITGHGDVPM
                     SVRAMKRGATDFLTKPIDQTDLLQAVQAAIQKNGVRRKQAEERQQACDCIATLTPRER
                     DVMKLVVAGLLNKQIAQKLGICEATVKVHRRRVMKKMGVASVAGLVKICFLCDHAG"
     gene            complement(1018..2547)
                     /locus_tag="PK576_04185"
     CDS             complement(1018..2547)
                     /locus_tag="PK576_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE60821.1"
                     /translation="MGDDVRSGNGDGFVFATLRGAVVSRASASLTTRLGYAVEEVENR
                     SLKNFVHADDWPEMKRFLKRGAGTGGAQAEIGYRIRHKDGSWRYFVSTLVPSEDGIGE
                     PGTVLFVSRDVTKRKRLEDQIIQDQTLLKTVLDVVPVIICVRNKEGRYLLVNKQGENY
                     FGKPASSIIGKLAGEISPNTEKVAAYLVWEGEVIASGKPKYISEEILKNHVGEIHVFD
                     GLMVPIFVNNEQAVLFMARDNTERYNMERERRELRENLYRADRLGRGGEIAMALTHEI
                     SQPLTGILSNAQATQLMLAQKEPDLTEIANILADIVADVKHAGNVIKRLSSYLKKKKP
                     VLDRIDVNEAILEIIKLLRSDSELKNVAVSTELAANLPRIMADRIQIQQVVLNLIMNA
                     GYAMAEQPTDQRRITVKTFLDGMKAVMVSVCDTGPGIAQKNLPRLFDAHFTTKANGMG
                     IGLAICHSILEAHGGRIWAENNPHGGATFMFSLPSTKKNRRYVKNGNVAVRGSFGGGV
                     P"
     gene            3098..3493
                     /locus_tag="PK576_04190"
     CDS             3098..3493
                     /locus_tag="PK576_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE60822.1"
                     /translation="MAKILIVDDERTILLTIQHLLNEQGFETCEASNGLAALQLFQTQ
                     FFDLVISDLRMPRMDGVSFLHEVKNLDPTVPIIVLTAYATSEILSAANERHAFTYLPK
                     PFQSETLLRIVGELLSAAPRGTDQAAVRA"
     gene            3567..4205
                     /locus_tag="PK576_04195"
     CDS             3567..4205
                     /locus_tag="PK576_04195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE60823.1"
                     /translation="MRVRHIIWDWNGTLLDDTRAGLQAVNAMLEHRGLPALDLTTYRE
                     IFGFPVRTFYRSAGFKLEEEDWDALAREFHDYFQADTTLRLHAHAVEVLEHIRRLGIG
                     QSVLSASEQTLLRTMLHDFGVTRFFEGVFGVDNLYGHSKLELGRELLARLALHPSAVL
                     LVGDSLHDHEVAADLGVACVLISQGHQSAARLARSGAPILHSLAELPVWLED"
     gene            4282..5223
                     /gene="cysK"
                     /locus_tag="PK576_04200"
     CDS             4282..5223
                     /gene="cysK"
                     /locus_tag="PK576_04200"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE60824.1"
                     /translation="MPYFTDNSLSIGNTPLVRLHTLTQGLRATVLAKVEGRNPSYSVK
                     CRTASSMIWEAERDGLLKPGMGIVEPTSGNTGIALAMVGAARRYPVTLTMPENMSTER
                     RRILAAFGARLDLTPATAGMAGALQRAQELFNDSSGKLFMPNQFANPANPLIHERTTG
                     PEIWSQTEGQVAAVIAGVGTGGTLIGTGRYFRNIGAEVSMVAVEPDESPLLTQRAAGA
                     PLNPSGHGIQGIGANFIPQIVDFSLIGRIETVTTQEAMETARRLACEEGLFSGISSGA
                     AVAAALRLAADDAFAGKQLVVILPDTGERYLSTSLFT"
     gene            5241..6497
                     /locus_tag="PK576_04205"
     CDS             5241..6497
                     /locus_tag="PK576_04205"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOE60825.1"
                     /translation="MTVAFKTFGCRLNQAETAQYAAAFAAAGIANVPFGMPADIVVVH
                     TCTVTRTAEDTCLKQIRQLRRNAPEALLVVSGCAVETVAAQTFDALTVDLVIPREHKD
                     ELVARVLRHLGIDTPRPPVPCRPLAHRATLKIQDGCDFACAYCIIPHTRGKPQSRPFD
                     ACVQEARERIAAGFAEIVVTGCNIACYADAGRRLPDLLTALATLPGLGRLRIGSIEPG
                     TVEREVIAQMAECPTICRFLHLPIQSGDNAVLARMRRRYTIEEVAATVSDAAQRMPDL
                     GLGADLIAGFPGETPERFERTKHWVSQLPFNNLHVFPFSERPGTAAATFDGTVPPAER
                     KRRVRELNRIRTRLREAFAEQWVGRTVRVLIERIDSAGRGSGWTGEYLACRVEGLPAS
                     AEGRLVSCVPYARDADLLLARSLQQS"
     gene            6628..7563
                     /locus_tag="PK576_04210"
     CDS             6628..7563
                     /locus_tag="PK576_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE60826.1"
                     /translation="MSKPDPSPKPGLSLTSFFSADDILTHLEQLTRNDCIRRILTHLS
                     DVHGFNDTDAYYDAILEREDATHTVVANGIAIPHARIMDLQRPYVGIATSEQGIPFAE
                     DEPPVHLILLVLIPRDQPGLYLQILRALASILRDRTAAKAVSRLRTPDEVMRFFERGG
                     MVLPDFVCAADIMEDTFISLRDNDNLKTCIDCFISQQINEIPVLDRDGDMVGVVRAGA
                     LLKVCLPEYLLWMSDLSPIVNFEPFTTVLRNEQHSCLSDILLQNFASVQMKAPAISVA
                     GEMIRHNASRCYVLNGKKLIGVITLSRFLSKIFRE"
     gene            7574..9082
                     /locus_tag="PK576_04215"
     CDS             7574..9082
                     /locus_tag="PK576_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOE60827.1"
                     /translation="MYSEPVVTLPAPRILIKIGFMAAITALAGWGAHLIGLAPQPLAV
                     VAVCTFVSVICTTLFFWTHRVAVAFLGVAVLIGSRAMTLHGMLRATELDIIFFLVGMM
                     VIVGALKDLGFLTWIIQSILNRKRMTGTSFTVILCFLSALLSSVVDEVSSIVVVLALV
                     FQVCDTLKIKPTPFVLIAVLCTNIGSAATMLGNPVGIFIGNKAGFNFSQFLAGASPIA
                     FVSLCATLCVVLLWFRKPIHEMTEKMDEHRTAHHGLAPMIRIPYRRGLFVLLATVAII
                     AFHHQIENILGLTGTENKNAFLIITPLIIAGLLMIYRPQQARHYIEHDVEWWTLLFFM
                     LLFSIAGSLEEQGITRNLALRLSEQVNGGPRAMTPLVLLISSLGSAFVDNIVFVAAFT
                     PVIQTLVERSAEFSILWWALLFGACFGGNITAIGSTANIVALGLLEKRGHQHVAFFEW
                     LKIGVLVGLITGLIAWGMLSVMTMPQPVRQPARESVVEGPLHEPHLSPVPTS"
     gene            9116..12280
                     /locus_tag="PK576_04220"
     CDS             9116..12280
                     /locus_tag="PK576_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOE60828.1"
                     /translation="MIAWGGPEVYAQAERLAKRGAVLQAELCGDSVSGVIAREGASEI
                     RTRFRLLANGLIESQCPCPTHVRSGLVCPHVVALGITLMLRKTDPQREAKYREDLRRA
                     RRIAQIPPENYLQRGADGLPARIVLTLSPDWVSEFRNAEVGVRLQLDLPSGLTDPAAV
                     PRGSKLALPAADDILLTVIEDIVGGPPPERFTVDAADFLNLLDVASQAILRTAGSETL
                     TVEQIPMKTLLKVDLDPDSGDLIVYPFAELPFLQTGSLPVFLVYGRRGRILANGHLWP
                     MENVLPIPYHSIYCRDEVITRDRVLTFLHRDLPALEAHTPVMMEVPPDLFVTEPGEPV
                     FRLTLQGSQASLRATLSACYGPHTLPAGSPETQGELALPDPADILKYRTRNSAAEAAA
                     CRLLTAYGFEGPPPEYTLVGTRPVLNFLGTGYRSLGRAGWKITFTGKLSSLIDTLPVI
                     TPVVRINETASGWFDVGFDFDRQNGPLLPPAEIQRALNRGDAFLEHHGTTLLIDREAV
                     DAMRAVFEDCPSRESPVPGHFLLPPVYAPFVHASLNALDGIDVEDPPDWRDRASARNR
                     DAHTRLTPIPLGMLENTLRPYQKEGVYWLRFLEEAGFNGILADEMGLGKTVQTLAWLS
                     LQRSDAETRGKTALIVCPTSLVENWNREAKTFVPHLRRVILSGPDRATLFPRVAEYDL
                     AITSYALLRRDLEAYRALRFAVVVLDEAQHIKNRSTQNAVAAKQIHAVGKLVLTGTPV
                     ENSVADLWSIMDFLMPGYLGEYEGFRLGYELPIAAGGREGELAQAKLRRKLHPFLLRR
                     LKRDVAHDLPDKIVRVSYCRLTSDQQKVYSDLLAVSRRSIGDLVRTKGFDRSRMEILA
                     MLMRLRQACCHLDLLKDHHPPGSYAAPSSKFEAFFELLDEAMDGGHRILVFSQFVTLL
                     KLLRAELDQRGIPCCYLDGATQDRLAQCQRFNLTPSIPVFLISLKAGGTGLNLTGADM
                     VIHLDPWWNPAVEDQATDRAHRIGQKRTVYSIKLIAEHTVEEKVLALQRKKQAVIQAT
                     LGTTDAALMRTLSFEDIRDLVGLT"
     gene            12731..13990
                     /locus_tag="PK576_04225"
     CDS             12731..13990
                     /locus_tag="PK576_04225"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE60829.1"
                     /translation="MSIASPCRAQAESPYPIVWRDLTQIQNALGTGDPHWDGESLLFS
                     NEQHQIRFFPGRRKADVNGTTVWLNAPATGSIAARDWRLAGTDLDLLQISLLPTPEAD
                     SVPEPLRVMLDPGHGGDDTGASCGNPAVREKDLTLAIAKRVGELLKRSGMRVAYTRTR
                     DVTLSLDERVRLARKHRPDLFVSLHANHAANTNATGVETYVLPPSGFPGTAEGSPARG
                     WQIGNRNDYHNTLLGFAIHRRLAALTNTVDRGLKRQAFFVLRENGCPATLLEFGFLSN
                     RDETLLMKTEAWQAACAEAIVEGILGYARRVTALDRAVAEKRARDAELNERWRRHLAE
                     RDRASQPAPTEAAPPPAARETVPEVPPSASLSAPPPRRNTDTGLSDARIARVSGTAPA
                     VPILRGTNVAPAELNTLIDFYGAGTVE"
     gene            13996..15768
                     /locus_tag="PK576_04230"
     CDS             13996..15768
                     /locus_tag="PK576_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60830.1"
                     /translation="MQNPPDPVPTETGSNHTPPTHLAHTLSLAAALVLIPALLGVLAM
                     LAFQKFNPRPRPPAAGVAEEMPFTGGTDTVERLTLWRRDLRHEGLPIEEANLRIWAAR
                     ATKPEILFRIAPPAPNQSWLWFVTQDGLHALAVSRQIDVLERHTVGLCDLITTQWVWT
                     VSMRWPECYAQPAVIGGHTLVRYAANGQRFAMELSPKGQILNLDALGQGMVEVWQPPP
                     PQTRFPGTPVAVTRNVFFAADEESGSLLGYAIESLPGLRYAGKGDAHTVCSGNGLLIF
                     RAREGRITVTDTLTQTVLAEYNAWRHTPDTAVTATLASPDGSQFTVFLLTRFPGEPPV
                     EREWCVILDLPEGRARPFFHADALSSRPQPPADPLQTISPDGRWCLSLTEGNVLRIAA
                     GAERREWAHCALAPLTGVRQPFDALTFLDEGRHVLLRQRDNLWLLDFATARQYADLLA
                     RIHASDAAVASGVVTEPVTNAYDTFPMLPDRDATRTPADSARLALHAERCAANQAWFY
                     AAALLDASLRHPVWEGRTPSANRFMLARYHILAGQHAAARRNLLIALKQLDLPPTLGS
                     SPVNDAPLMRFHLQRLLQALPSGR"
     gene            15952..16614
                     /locus_tag="PK576_04235"
     CDS             15952..16614
                     /locus_tag="PK576_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60831.1"
                     /translation="MKTQHLLFPVLLTALVTTADFSFPLFNGRDLSGWKVADMIGAGS
                     VTVLSDGTVECGFGTPMTGIAYTNAMPTLNYEIELEAMRVQGTDFFVALTLPVETNFC
                     TVIIGGWGGGLCGVSSVDYMDASENQWSEHVTFENNRWYRLRVRVTPGVLQVFLNDDL
                     YSARIEFDSSTRFSLRPGSDIDKTTPLGLATYCTRARWRHASLTRITTLTPADKPRSE
                     EP"
     gene            complement(16637..17302)
                     /locus_tag="PK576_04240"
     CDS             complement(16637..17302)
                     /locus_tag="PK576_04240"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE60832.1"
                     /translation="MERQTRRGGTTTGRSRTRNRILRAATKVFAEYGFREATTRMICA
                     EAAVNSALVNYHFRSKAELYRAVIVSLFEHIGKPMMRINETVTDEASWKAAVRTWIER
                     ALAICAASKPPEVWAARLMGMEECVPSELAQDIETKFAKPVRQCFQRLLRMGMKRDDP
                     VEIGLWASAIHAQCVVFALTRPGWVERFCPPGTSREDWLRRVTEHICEGVFSRLSYQG
                     ARK"
     gene            complement(17321..18226)
                     /locus_tag="PK576_04245"
     CDS             complement(17321..18226)
                     /locus_tag="PK576_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE60833.1"
                     /translation="MRRYRLTKAGFEVSRLAYGTWRLLADKPTAQEVNRRLHLCLELG
                     ITTIDTAEIYGKYGVEALLGEALALSPGLREQLEIVTKAGIYVPCEACPERRTAHYNA
                     TAEQLCASLDQSLKRLRTERIDLFLIHRPDWLTRTEDTAQGLNRLVQEGKIRAAGVSN
                     YAPAGVDLLNACLDRPLAVNQIAFSLLHMEAMEDGTLTQCERLGMTPMAWSPLAGGRL
                     FDPAEPAAGRLAAAAAACAVRYGGVTLDQFAYAWILAHPSHPMAIIGTNRLDRLRLAA
                     QADALTLEREDWYAFWEAAQGRRIP"
     gene            complement(18236..>19901)
                     /locus_tag="PK576_04250"
     CDS             complement(18236..>19901)
                     /locus_tag="PK576_04250"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60834.1"
                     /translation="NTREGMTRPGQWYLDRAAGRLVYWPLAGERMESASVIAPTLTSV
                     IRLRGSAQAPVERVTLRGFSVQATTTPLKPGGFSAHAYDGAVELYHAKECALIGMEIC
                     HVGGQGIKSWALDACRIEACHIHHVGACGIRTGGDASVIEGNHIHHAGVYQPSAVALQ
                     FGRVRRDSKLSGFHIRRNEIHDAPYSGMTGGGSDHLIEENLIYRVMRELHDGAAIYGS
                     MSRTVLRGNVVRDVVEIGQGYGASAYYFDEGATGNVVERNVAIGVPMPTHNHITTGVT
                     IRDNVFVSDGDMKLSFPRSRDCVFTGNTLYAPGKVTVTAPNAVTSWVGNVLFSGNAQG
                     GSTPAAFAITDAMPPAPAPARRTWAVAVPRTEQPPVMDGEIGDGEWAGSFVNIDRDPS
                     RWLASGAPAFIKLMYDEQYLYVAANTVLFDIGLLSKGDRWGQDDGAELCIAGAKGIAV
                     IRGFAGGACVSVTDAGLPSEEAERLGKAVRFAAKPYGKTRNDWKSGWRGEWAIPFKEL
                     GIRPEPGMKVAFNYGLFRAEDKVWRSLEGTGAANWRLSEAALLQFK"
BASE COUNT         3753 a         6746 c         5509 g         3893 t
ORIGIN      
        1 cgccggggaa cccggctttt gcgattcgct cacaggtcgc atcgtccgcc acctgcgcga
       61 tcagagcttt ttcgagcttc gttttgtatg cggagcccgc cgccgcatgt gatgccgcag
      121 gcagtcccgc cacgatcccc gtcacggcgg cggccttcag aaagtcacgt cggttcattt
      181 gctttccttt accttcgtcg ttttcactct cgacacatgg tctacgccca tttagcaagg
      241 ccagtataac acagacagac cccgcgaacc cctcaaaata tgcaaaaaga ttgtcgcgtc
      301 taagatgtcg gggcttgcag ccgaaagctc ggcgatagtc cgggacattg cgtcggatga
      361 gaaccccggt tcaaccggcg tgatcgcata gaaaacagat tttaaccagt cctgccactg
      421 aggcgacacc cattttcttc atcactcggc ggcgatgcac cttgacggtc gcctcgcaga
      481 tgcccagttt ttgggcaatc tgcttgttga gcaggccagc cacaaccagt ttcatcacgt
      541 cgcgctcgcg cggggtcaga gtggcgatac agtcgcatgc ctgttgccgt tcttccgcct
      601 gcttgcgccg gacaccattc ttttgaatgg ccgcttgaac cgcttgcagc aaatccgtct
      661 ggtcgatcgg ttttgtcaga aaatcagtgg caccccgctt catcgcccgc acactcatgg
      721 gaacatcgcc atgccccgtg ataaaaacga tgggcagagg cgaatcatct ttggcgagtt
      781 cctcctgaag cgccaagccg tccattccag tcatccgaac atcgagcacc aggcacccgt
      841 ccccatcaaa ccgcggccgg tctaagaact cttgggccga tgcgaaggtt attacctcat
      901 agcccgcggc ttgcaacagg cgcggcagcg ccttgcgcac cgaggggtcg tcgtcaacca
      961 gaaatatcgt cggccggatc gccgcggatg cggtttcgga tacaggatcg ttcgttttca
     1021 tgggacacct cctccaaaag agccccttac tgcaacgtta ccgtttttca cataccgtcg
     1081 gttttttttc gtgctcggca gcgaaaacat gaacgtggcg ccgccgtgcg gattgttctc
     1141 cgcccatatc cgtccgccgt gcgcctccag aatagagtgg caaatcgcaa gcccgatgcc
     1201 cattccgttc gccttcgtcg tgaagtgcgc atcgaacaac cggggcaggt tcttctgagc
     1261 aatccccggt cccgtatcgc acaccgacac catcaccgct ttcatgccat ccagaaacgt
     1321 cttcaccgtg atgcgccgct gatccgtcgg ttgctccgcc atggcgtacc cggcattcat
     1381 gatcaggttt agcacgactt gctgaatctg gatccgatcc gccatgattc ttgggagatt
     1441 cgcggccaac tccgttgaca ccgcgacatt tttcaattcg gaatcggacc gaagcaattt
     1501 gataatttca aggatcgcct cattgacatc gatccgatcc agtacgggct tcttcttttt
     1561 caaatacgaa ctcaaccttt taatcacatt tccggcgtgt ttaacgtctg ccacaatgtc
     1621 agccagaatg ttcgcaattt ccgtcaaatc cggctctttc tgcgccagca taagctgggt
     1681 tgcctgcgcg ttactcaaga tacccgtcaa cggctggctg atctcgtgtg tcagggccat
     1741 agcgatctcg ccgccccgcc ccaaacggtc cgcacgatac agattctcgc gaagctcgcg
     1801 cctttcccgt tccatattgt agcgctcggt gttgtcacgc gccatgaaca aaacagcctg
     1861 ctcgttattg acgaagatag gcaccatcaa accatcaaac acgtgaattt cgccgacgtg
     1921 atttttcaag atttcttcag agatatattt cggttttccg gacgcgatga cctcgccttc
     1981 ccagacaaga taggcagcca ctttttcagt gttgggacta atttccccgg ccaacttccc
     2041 gataattgaa gatgcgggtt tcccaaagta attttctccc tgcttgttca ccagcaagta
     2101 acggccttcc ttgttcctaa cgcaaatgat gacaggcacg acatccagaa cagtcttcag
     2161 taatgtctga tcctgaatga tttggtcttc cagcctcttt cgtttcgtga cgtctctgga
     2221 aacgaacaac acggttccag gttcacctat tccatcctct gaaggcacca gcgtcgacac
     2281 aaaatatcgc cagcttccgt ccttgtggcg gatccgatac ccgatttcgg cttgggctcc
     2341 ccctgttcca gcgccccgtt ttaaaaaccg cttcatttcc ggccagtcgt cggcgtgtac
     2401 aaaattcttg agtgatcggt tttccacctc ctcgaccgca tagcctaaac gggtggtcaa
     2461 ggacgccgaa gcccgggaca caacagcgcc ccgcagcgtg gcaaacacga atccgtcgcc
     2521 gttacctgat cgaacgtcgt cccccacaaa aacctcccta gcgattcact ccgaccgaaa
     2581 ccgacgacca cccggcacaa gccgggcggt ctcgttctcg acctcgctca cggtcggcgg
     2641 aaaggttcct tgaaagtatt accatccccc aagacaggcc agaaacattc cggttccaaa
     2701 gattggcatc tatcatgcaa gggaagggtg aaaaccgtca acccctaaaa tttctcaaga
     2761 cgtgtcatcc gaaaagggac gggcgatcgg caccttaccc tgagacccgg ttcgcggaat
     2821 tctcaagcgt gtgcctcgca cacccccgtt cttttctaaa aaatgacaaa accgggcccg
     2881 ccccgcatcc cccggcatcc gctcccgaaa cttttgtgct tcacgaggcc cgaactcgaa
     2941 aacgacgtta tttcgttcat tttgcatccc tcctccgcat gcgtcgcgcc gccggtcctc
     3001 cgggaaggga tcgaaatggc acaccttttg ctagaatcat ttgcgtattt gcgtatcgac
     3061 cctgacaaac cgatgatgtc ttggggaacc gcttgtaatg gctaagatcc tgatagtcga
     3121 cgatgagcga acgattctgc tgacgattca gcatcttttg aatgaacagg gatttgaaac
     3181 gtgcgaggca tccaacgggt tggccgcact ccagctcttc cagacccaat tcttcgatct
     3241 ggtgatctcg gacctgagaa tgccgcgcat ggacggcgtc tcctttctgc atgaagtcaa
     3301 aaacctcgac ccgaccgtac ccatcattgt tttgacggca tacgccacgt ccgaaatcct
     3361 ttccgcagcc aacgagcggc acgctttcac ctatctgccc aagccctttc agtccgaaac
     3421 cctgctccgg atcgtgggcg aattgctgtc cgccgcaccc cggggcacgg atcaggcggc
     3481 ggtgcgggcc tgaatcggac ccgcggttca caggacgggt gacccgcgac gccaccggga
     3541 aggcgataaa ggaaccgtct gcaagtatgc gtgttcgcca tattatttgg gattggaacg
     3601 gaaccctgct cgatgacacc cgggcgggtc tccaagccgt gaacgccatg ctcgaacacc
     3661 gcgggctgcc tgcgctggat ctcacgacct accgggagat tttcggcttt ccggtccgga
     3721 ccttctaccg gtctgcggga tttaaattgg aggaggagga ctgggacgcc ctggcccgtg
     3781 aattccacga ctattttcag gccgacacca cgctgcggct gcatgctcac gcagttgagg
     3841 tcctggagca catccgacgc ctcggaatcg gccagtccgt gctctcggct tcagaacaga
     3901 ccctcctccg caccatgctg cacgatttcg gcgtgacccg tttttttgaa ggcgtcttcg
     3961 gggtagacaa tctgtacggg cactccaaac tggaactggg gcgcgaactg ctggcacggc
     4021 tcgcgctgca cccgtcggcg gtgctgctgg tcggcgactc cctgcacgat cacgaggtag
     4081 cggcggatct gggcgtggcc tgcgtcctga tctcccaggg ccaccagtcc gccgcgcgct
     4141 tggcccgcag cggcgctcca attctgcaca gtctggcgga actccccgtc tggttggaag
     4201 actgaaccga tgcgtaaaat gctttgtcct ttgtcgccgc aggggaacct gttaaagtga
     4261 ttggattcag aagagggtgc catgccatat ttcactgaca attcgctgtc gatcgggaac
     4321 acaccgctcg tgcggctcca tacgctcaca cagggattgc gggccactgt gctcgccaaa
     4381 gtggaggggc gcaacccctc ctattccgtc aaatgccgga ccgcctcatc catgatttgg
     4441 gaggccgaac gggacggcct gctcaagccc ggcatgggga tcgtcgaacc caccagcggc
     4501 aacacaggca tcgccctcgc catggtgggc gccgcgcggc gttatccggt cacgctgacc
     4561 atgccggaaa acatgagtac ggaacgccgg cgcatcctcg ccgcgttcgg cgcgcgcctg
     4621 gatctcacgc ctgcaaccgc aggcatggcc ggagcgctcc aacgggcaca ggaactgttc
     4681 aacgactcct ccggaaaact cttcatgccc aaccagttcg ccaaccccgc caacccgctc
     4741 atccatgaac ggaccaccgg accggaaatc tggagtcaga ccgagggcca ggtcgccgct
     4801 gtgattgcgg gggtgggtac cggcggaacc ctgatcggca ccggaaggta tttccggaac
     4861 atcggcgcgg aggtgagcat ggtggcggtc gagccggatg aaagtcccct gctgacgcaa
     4921 cgtgccgcag gggcacccct caatccgtcg ggccacggca ttcaggggat cggcgccaat
     4981 ttcatcccgc agatcgtgga cttctcgctg atcggccgga tcgaaaccgt gaccacgcag
     5041 gaagcaatgg aaaccgcacg gcgtctggca tgcgaagaag gcctcttctc gggcatttca
     5101 agcggcgcgg cggtcgccgc ggccctgcgc ctggccgcag acgatgcgtt tgcgggaaaa
     5161 cagttggtgg tcatcctgcc ggacacgggc gagcgttacc tctccacgtc cctcttcacc
     5221 tgaccgcatt gccgaagctc atgacggttg ccttcaagac cttcggatgc cggctgaatc
     5281 aggccgaaac cgcacagtat gcggccgcat tcgcggcagc gggcatcgcg aatgtgccgt
     5341 tcggcatgcc cgccgacatt gtggttgtcc atacctgcac ggtcacgcgc acagcggaag
     5401 acacctgcct gaagcagatc cggcaactgc ggcggaatgc gcccgaagca ttgctcgtgg
     5461 tctcgggatg cgcagtggaa accgttgcgg cgcaaacgtt cgacgcgttg accgtcgacc
     5521 tcgtgatacc gcgtgaacac aaagatgaac tggtggcgcg tgtgctgcgg caccttggca
     5581 tcgacacccc ccgtccgccg gtaccctgcc gacctctcgc ccaccgcgcc accctgaaaa
     5641 tccaggacgg gtgcgatttc gcctgcgcct actgcatcat tccccacacg cggggtaaac
     5701 cgcaaagccg cccttttgac gcatgcgtgc aagaggcgcg ggaacggatc gcggcgggtt
     5761 tcgccgaaat cgtggtgacc ggatgcaaca tcgcctgcta tgcggacgcc ggacgccgtc
     5821 taccggacct gctgaccgca ctcgccaccc tgccgggcct cggccgcctt cgtatcggct
     5881 cgatcgaacc cggcaccgtc gaacgcgagg tgatcgcgca gatggcggaa tgtcccacca
     5941 tctgccgctt cctgcacctg cccatccaga gcggcgataa cgccgtcctc gcgcgcatgc
     6001 ggcgacggta tacgatcgag gaggttgccg caaccgtttc cgatgcggct caacgcatgc
     6061 ccgatctcgg tctcggcgcg gacctgatcg ccgggtttcc gggcgagacc ccagagaggt
     6121 tcgagcgcac gaaacactgg gtttcccaac ttccgttcaa caacctgcac gtctttccgt
     6181 tcagtgagcg tcccggcacg gcggccgcca cattcgacgg caccgtcccg ccggccgaac
     6241 gcaaacggcg cgtacgggaa ctgaaccgta tccggacccg gttgcgcgag gccttcgctg
     6301 aacaatgggt cggacgaact gtccgcgtgc tgatcgaacg catcgacagc gcgggccgcg
     6361 gaagcggatg gaccggcgaa taccttgcct gccgcgtgga gggcctgccc gcatcggcgg
     6421 aagggcggct ggtatcctgc gtcccgtacg cgcgggacgc agatcttctg ctggcccggt
     6481 ccctgcaaca aagttgaatc gtatccaaag gaaaggttct gcgttgtttt tccgcaaccg
     6541 aaacggtata gtcatcgttg ttcggtccgg aatcgcggca gacgcaaaag aaggacccac
     6601 ccgaccagcg gtgaacggaa cgtagacatg agcaaacctg acccatcacc caaacccggc
     6661 ctgtccctca cgtcgttctt cagtgcggat gacattctga cgcatctgga gcagctcacc
     6721 cgcaatgact gcatccgccg cattctcacg catctctccg atgtgcacgg gtttaacgat
     6781 acggatgcct attacgatgc aatcctcgag cgcgaggatg ccacccacac ggtggtggcg
     6841 aacggaatcg ccattccgca tgcgcgcatc atggatctgc agcggcccta tgtcggcatc
     6901 gcgacctctg aacagggcat cccctttgcg gaagatgagc cgcccgtgca tctgatcctg
     6961 ctggtgctga tcccgcgtga ccagcccgga ctctatctcc agatcctgcg cgcgctcgcc
     7021 agcatcctgc gcgaccgcac cgcggcgaaa gcggtttcac gcctgcgcac gccggatgag
     7081 gtcatgcggt tcttcgagcg gggcggcatg gtgctgccgg attttgtgtg cgccgcggac
     7141 atcatggagg acaccttcat ctccctgcgc gacaatgaca acctgaaaac ctgtatcgac
     7201 tgtttcatat cgcagcagat caacgagatt ccggtgctcg accgggacgg ggacatggtc
     7261 ggtgtggtgc gggccggcgc cctgctcaag gtctgcctgc ccgaatacct tctttggatg
     7321 tcggatctct cccccatcgt caatttcgaa ccgttcacca ccgtgctgcg caacgaacag
     7381 cactcttgtc tctccgacat cctgctgcag aatttcgcct cggtgcagat gaaggccccc
     7441 gccatttcgg tcgccggcga gatgatccgc cacaatgcct cccgctgtta cgtgctgaac
     7501 ggcaagaagc tgatcggcgt catcaccctg tcgcgttttc tgagtaaaat tttccgggag
     7561 tgagttttcc gatatgtaca gcgaacccgt cgtcacctta cccgcacccc gcattctgat
     7621 caagatcggt ttcatggccg ccattaccgc gttagccggt tggggcgccc atctgatcgg
     7681 actggcgccc caaccgctgg ccgtggtcgc ggtctgcacg ttcgtcagcg tcatctgcac
     7741 cacactgttc ttctggaccc accgtgtcgc cgtggccttt ctcggcgtcg ccgtgctgat
     7801 cggatcgcgc gccatgacgc tgcacggcat gctgcgcgcc accgaactcg acatcatttt
     7861 tttcctcgtc ggcatgatgg tgatcgtcgg cgccctcaaa gacctcgggt tcctcacctg
     7921 gatcatccaa tccatcctca accggaaacg catgaccggc acctccttca ccgtgatcct
     7981 ctgctttctc tcggccctgc tctccagtgt ggtggacgag gtctcctcga tcgtggtggt
     8041 cctcgcgctc gtttttcagg tttgcgacac cctcaaaatc aagccgacgc cgtttgtcct
     8101 gatcgcggtc ctctgcacca atatcggctc ggccgccacc atgctcggga accccgtcgg
     8161 cattttcatc ggcaacaagg ccggattcaa cttcagccaa ttcttggcgg gcgcctcccc
     8221 gatcgcgttc gtctccttgt gcgccaccct gtgcgtggtg ctgctgtggt tccgtaaacc
     8281 gatccacgag atgacggaaa agatggacga gcatcgcacc gcccatcacg ggctggcccc
     8341 catgatccgt atcccgtacc gccgggggct gtttgtcctg ctcgccacgg tcgccatcat
     8401 cgcgttccac catcagatcg aaaacattct cggcctgacc ggcacggaaa acaaaaacgc
     8461 cttcctgatc atcacgccgc tcatcatcgc cggcctgctc atgatctacc ggccgcaaca
     8521 ggcgcgccac tacattgaac acgatgtgga gtggtggacc ctgctcttct tcatgctgct
     8581 cttttccatc gcgggctcgc tggaagagca aggcatcacc cgcaacctgg ccctccgcct
     8641 cagtgaacag gtcaacggcg gaccacgcgc catgacgccg ctcgtgctgt tgatctcctc
     8701 cctcggttcg gcatttgtcg acaacatcgt tttcgtggcc gccttcactc ctgtgatcca
     8761 aaccctggtc gaacgttccg ccgagttctc gattctctgg tgggcgctgc ttttcggcgc
     8821 ctgcttcggc ggcaacatca ccgccatcgg ctccacagcg aacattgtcg cgctcggcct
     8881 gctggaaaaa cgcggacacc agcacgtcgc ctttttcgaa tggctcaaaa tcggtgtgct
     8941 ggtcggattg atcacggggc tgatcgcctg gggcatgctg agtgtcatga ccatgccgca
     9001 acccgtacgc cagccggccc gggagtccgt ggtggaaggt ccgttgcatg agccgcacct
     9061 ctcccccgtc ccgacatcct gacgtatgcc gctttccgcc ataacccgga aacaaatgat
     9121 cgcgtggggc ggtccggagg tgtatgcgca agcggaaagg ctggcaaaac gcggcgcggt
     9181 gctccaagcc gaactttgcg gcgattctgt ttccggtgtg atcgcgcgcg aaggcgcgtc
     9241 cgagatccgt acccgcttcc gcctcttggc gaacgggctg atcgaaagcc agtgcccctg
     9301 ccccacccat gtccgcagcg gcttggtctg cccgcacgtc gtggccctcg gcatcaccct
     9361 gatgctgcgc aagaccgatc cgcaacgcga ggcgaaatac cgggaggacc tgcggcgcgc
     9421 ccggcgcatc gcccaaatcc ccccggagaa ttacctgcaa cggggcgcgg acggtcttcc
     9481 cgcccggatc gtgctcaccc tctctccaga ctgggtctcc gaattccgta acgcggaggt
     9541 gggggtccgg ctgcaactcg atctgccttc cggcctcacc gacccggccg ctgtcccgcg
     9601 cggttcaaag ctcgcgctcc cggccgcgga tgacatcctg cttaccgtga tcgaggacat
     9661 cgtcggcggt cccccgcccg agcgcttcac agtggatgcc gccgattttc tgaatcttct
     9721 ggatgtcgcc tcgcaggcga ttctccgcac agccggatcc gaaaccctga cggtcgagca
     9781 gatccccatg aagaccctgc tcaaagtgga tctggatccg gattcgggcg acttgatcgt
     9841 ctaccccttc gcggaacttc cgttcctcca aaccggcagc ctgcccgtct tcctcgtgta
     9901 cggacggcgc ggccgcattc tcgccaacgg tcacctgtgg cccatggaaa acgtcctgcc
     9961 catcccgtac cacagcatct attgccggga cgaggtcatc acgcgggatc gcgtcctgac
    10021 ctttctgcac cgtgatctgc ccgctctcga agcgcacacg ccggtgatga tggaggtgcc
    10081 gcccgacctg ttcgtgaccg agcccggcga accggtcttc cggctcacgc tgcaaggctc
    10141 ccaagcctcg ctccgggcca cgctctccgc ctgctacggc cctcacaccc tccctgccgg
    10201 atccccggaa acgcaagggg aactcgcatt gccggatcct gcggacatcc ttaagtaccg
    10261 tacacgcaac tccgccgccg aagccgctgc ctgccgcctt ctgacagcgt acggttttga
    10321 gggccccccc cccgaataca cgctggtcgg cacacggccg gtactcaatt tcctcggcac
    10381 cggttaccgg tctctgggcc gcgccggatg gaaaatcacc ttcaccggaa aactgtcctc
    10441 gctcatcgac accctgccgg tcatcactcc ggtggtccgg atcaacgaga ccgcctccgg
    10501 ctggtttgac gtggggttcg attttgaccg gcagaacggc ccgctactgc cacccgccga
    10561 aattcagcgc gccctcaacc gcggcgacgc gtttctcgaa catcacggca ccaccctgct
    10621 gatcgaccgg gaggccgtag acgccatgcg cgccgtgttc gaggactgcc cgagccgtga
    10681 aagccccgtt cccggccact ttctgttgcc tccggtctac gcccctttcg tgcatgcctc
    10741 gctcaacgcg ctcgacggga ttgacgtaga agacccgccg gactggcgtg atcgcgccag
    10801 cgcacgcaac cgcgacgcgc acacacggct caccccgatc ccgctcggca tgctggaaaa
    10861 cactctccgc ccctaccaga aggagggcgt gtactggttg cggtttctgg aggaagccgg
    10921 atttaacggc attctggccg acgagatggg gctcggcaaa acggtgcaga ccctcgcctg
    10981 gctgagcttg cagcgaagcg acgccgaaac cagagggaaa accgcgctga tcgtctgccc
    11041 caccagcctg gtcgaaaact ggaaccgtga ggcgaagacc tttgtccccc atctgcgccg
    11101 tgtgatcctc tccggtcctg accgggccac gctctttccg cgtgtggccg aatacgacct
    11161 ggcgatcacc tcgtacgcgc tcctgcgccg cgatctcgaa gcctaccgcg ccttgcggtt
    11221 cgctgttgtc gtgctggatg aagcgcagca cattaaaaac cgttcgacac aaaacgccgt
    11281 ggcagccaaa cagattcatg ccgtcggcaa gctggtgctc accggcacac cggtggaaaa
    11341 cagcgtggcc gacctctggt cgatcatgga ttttctgatg ccagggtact tgggcgagta
    11401 cgagggattc cgcctcggct atgaactgcc catcgcggcc ggcgggcgcg agggtgaact
    11461 cgcgcaagcc aaattgcgcc gcaagctcca tccctttttg ctgcgccgcc tcaaacggga
    11521 tgtggcccac gacctgcccg acaaaatcgt ccgggtttcg tattgtcggc tcacctccga
    11581 ccagcagaag gtgtattcgg atctgctcgc cgtatcacgc cgctccatcg gcgatctggt
    11641 ccggactaaa gggttcgacc gctcgcgcat ggagatcctt gccatgctca tgcgcctgcg
    11701 tcaagcctgc tgccatctgg atctgctgaa agaccaccac ccgcccggct cctacgcagc
    11761 gccctcatcc aaattcgagg cttttttcga actgctcgac gaagcgatgg acggtggcca
    11821 ccgcattctg gttttcagcc agttcgtcac cctgcttaaa ctcctgcggg ccgaattgga
    11881 tcagcgcggc atcccctgct gttatctgga cggggccaca caagaccggc tggcccaatg
    11941 ccaacggttc aacctgactc cctcgattcc ggttttcctg atcagtctga aggccggcgg
    12001 caccggactc aacctcaccg gcgcggatat ggtgattcat cttgacccct ggtggaatcc
    12061 ggctgtggag gatcaggcca cggaccgcgc acaccgcatc ggccagaagc gcaccgtcta
    12121 cagcatcaaa ctcatcgcgg aacacacggt cgaagagaag gtcctagcct tacaacggaa
    12181 aaaacaggcc gtcatccaag ccaccctcgg aaccacggac gcagcgctga tgcggacgct
    12241 ctccttcgag gatatccgcg atctggtcgg cctgacctga accggttcgc cacagggaca
    12301 agtctggaga cacagatttc cccgcccgca gcagcgtgtc ttgcgctttc tggttttatc
    12361 cgcagatttt tcttttttgt ctttgaccgc aggcggggag ggacgagcct ctgttcgtcc
    12421 gggcggcgtt tcacgccgcc gcctttgaac gccgaacgcc caacgccgaa ctcagaacgt
    12481 cgaagtaaaa aaaactccta actcctaaat cctaaatcct aactcctaaa taaatcctac
    12541 atccgcgcgt agcgcggaag acgcccgcgc tccctttgcg cacacacaaa acacgcgttc
    12601 gactcgcatg cccctgtcac gaaccgattg cttcacaccc tcatttcttc cttcaacttc
    12661 ggcgctctct cccgtatact gtgtccgcat catgattggt tggcgtttgt ttctttttct
    12721 gttttgctgt ttgtcgattg caagcccgtg ccgcgcacag gctgaatcgc catatccgat
    12781 cgtctggcgc gatctgacgc agatccagaa cgcactcggg acgggggacc cgcactggga
    12841 cggcgaatcc ctgctcttct cgaacgaaca gcaccaaatc cggttctttc cggggcgccg
    12901 caaagcggac gtcaacggca cgaccgtctg gctcaatgct ccggccaccg gctcgatcgc
    12961 ggccagagac tggcggttgg cgggcacgga ccttgatctg ctgcagatct ccctgttgcc
    13021 aacgcccgaa gccgacagcg tgccggaacc cctgcgtgtg atgttggatc cgggacacgg
    13081 cggggacgac accggcgcat cgtgcgggaa cccggcggta cgggaaaagg atctgacgct
    13141 ggcgatcgcc aaacgcgtcg gcgaactcct caagcgttcc ggcatgcggg tggcgtacac
    13201 gcgcacgcgg gatgtcacgt tgtcgttgga tgagcgggta cgccttgccc gaaagcatcg
    13261 gcccgacctg ttcgtcagcc tccatgccaa ccatgcggcc aacaccaacg ccacgggggt
    13321 cgagacgtat gtgctgccgc ccagcgggtt tccgggtacg gcagagggga gcccggcgcg
    13381 cggctggcag atcggcaacc gtaatgatta tcacaacacc ctgctggggt ttgcaatcca
    13441 ccgccgcctc gccgctttga ccaacacggt ggaccgcggc ctgaaacgtc aagccttctt
    13501 cgtgctgcgc gaaaacggtt gccccgccac gcttctggag ttcggtttcc tttccaaccg
    13561 cgatgaaacc ctcctgatga agacggaggc ttggcaggcg gcgtgcgccg aagccatcgt
    13621 cgaggggatt ctgggttatg cgcgacgcgt cacagcgctc gaccgcgccg tggcggaaaa
    13681 gcgcgcgaga gacgcggaat tgaacgaacg ctggcgccgc cacctcgccg aacgggacag
    13741 ggcatcccaa ccggctccga cagaggcggc cccgccgcct gccgcgcgcg aaaccgtgcc
    13801 ggaagtcccg cccagcgcct ccctgtccgc accccccccg cgccgaaaca ccgataccgg
    13861 tctttccgac gcgcgcatcg cgcgcgtgag cggaaccgcc ccggccgtac cgatcctgcg
    13921 cggcaccaat gtcgcgcccg cggaactcaa caccctgatt gatttttacg gcgcgggaac
    13981 agtagaatga atgccatgca aaacccacct gacccagtac cgaccgaaac cggatccaac
    14041 catacccccc ccacccactt ggcacacacg ctgtcgctcg ccgcagctct ggtgctgatc
    14101 cccgcccttc tgggcgttct cgccatgctg gcctttcaaa agttcaaccc ccggccccga
    14161 cctcctgccg ccggagtggc ggaggaaatg cctttcaccg gtggaaccga tacggtcgaa
    14221 cggctgacgc tctggcgccg cgacctgcgg catgaggggc tgcccatcga agaagccaac
    14281 ctgcgcatct gggccgcccg cgccacgaag cctgagatcc tgttccgtat cgcaccgccc
    14341 gcgccgaatc agtcttggct ctggttcgtg acgcaggacg gtctgcacgc attggctgtt
    14401 tcgcgccaaa tcgacgtgct cgaacgccat accgtgggcc tctgtgacct gatcaccacg
    14461 caatgggtct ggaccgtctc tatgcgctgg ccggaatgtt acgcccagcc tgcggtcatc
    14521 ggcggtcaca cgctcgtacg gtatgcggcg aacgggcagc ggttcgccat ggaactcagc
    14581 cccaaaggcc agatcctcaa tctcgacgcg ctcggacaag gaatggtcga ggtctggcaa
    14641 ccgccgccgc cgcagacgcg ttttcccgga acgccggtgg ccgtgacccg caacgtcttc
    14701 ttcgcggcgg acgaagagag cggatcgcta ctggggtacg cgatcgaaag tctgccgggc
    14761 ctgcgttacg caggaaaggg cgacgcgcac accgtctgtt ccggcaacgg tctgttgatt
    14821 ttccgtgccc gcgaggggcg catcaccgtg acggacaccc tgacgcaaac cgtgctggcg
    14881 gagtacaacg cctggcgcca cacacccgat accgcggtga ccgccacgtt ggcttcgccg
    14941 gacggctcgc agttcaccgt gtttctcttg acccgcttcc cgggcgagcc gccggtcgag
    15001 cgcgaatggt gcgtcatcct agacctgccc gagggccgcg cgcgtccttt cttccatgcg
    15061 gacgcgctct cttcccgacc gcaaccgccc gccgatccgc tccagaccat ctcaccggat
    15121 gggcgctggt gcctgagcct gacggaggga aacgtgttgc gcatcgccgc aggcgcggaa
    15181 cgccgcgaat gggcgcactg cgcactcgct cccctcaccg gagtccggca gccgtttgac
    15241 gcgctcacgt ttctcgatga agggcggcac gtcctgttgc gccaacgcga taacctgtgg
    15301 ctgctggatt tcgccaccgc acgtcagtat gcggatctgc tggcgcggat ccatgccagt
    15361 gatgcggcgg ttgcgtccgg cgtggttacc gaaccggtca cgaacgcata cgacacgttc
    15421 ccgatgttgc cggaccgtga cgccacccgc acccccgcag actctgcccg actggcgctg
    15481 catgccgaac ggtgcgcggc caatcaggct tggttttatg ccgcggcctt gctcgacgcc
    15541 tccctccgcc acccggtatg ggagggacgc actcccagcg cgaaccggtt catgctcgcc
    15601 cggtaccaca ttctggcggg ccagcacgct gccgcgcgcc gcaacctgct gatcgcgttg
    15661 aaacaactgg acctcccgcc gactctcggt tcgtcccccg tcaacgacgc gcccctcatg
    15721 cgtttccacc tgcaacgttt gttgcaggcc ctaccgtccg gccgctaagg aagggggagg
    15781 acaaaagaca ggcgacgggt gtctcactgg cgggcggggg cgcccgcgca gcgggaaatc
    15841 ctgtcaatgt ataggcaagt tttaaccatc gcctcgttct tacaacctgt aacgtgtaac
    15901 cggcaatctg ttgttatcgc ttttcgtcat cgtatacgct atcctgaaac catgaaaaca
    15961 caacaccttc tgtttcccgt cctgctgacc gcacttgtga ccacagcgga cttttccttt
    16021 ccgctcttca acggccgcga tctctccggc tggaaggtcg ccgacatgat cggggccgga
    16081 agcgtgaccg ttctgtccga cgggactgtg gagtgcggtt tcggcactcc catgaccggc
    16141 attgcctaca ccaatgcaat gcccaccctg aattatgaga tcgaactgga ggcgatgcgg
    16201 gttcagggca cagatttctt tgtggcgctg accctgccgg tggaaaccaa cttctgcacc
    16261 gtgatcatcg gcggctgggg gggcggcctc tgcggcgtct cctccgtgga ttatatggac
    16321 gcctcggaga atcagtggtc ggaacacgtc acctttgaga acaaccgctg gtaccgtttg
    16381 cgcgttcggg tcacgcccgg tgtgctgcaa gtttttctga atgacgacct ctattccgcg
    16441 cgcatcgagt tcgactcttc cacgcgcttc agcctgcgcc ccggcagcga catcgacaag
    16501 accaccccgc tcggtctggc gacgtattgc acccgcgccc gctggcgtca cgcctcactt
    16561 acgcggatca ccacactgac ccctgccgac aaaccccgca gcgaggagcc gtaacgcggc
    16621 accatttccc gcgcactcat tttcgcgcgc cttggtagga gagacgcgag aacacccctt
    16681 cgcaaatgtg ttccgtcacc cgccgcagcc aatcttcacg gctggtgccg ggcggacaga
    16741 aacgttcaac ccatcccggc cgggtcaatg cgaacaccac gcactgcgca tggatggcgc
    16801 tcgcccaaag cccgatctct accgggtcgt cccgtttcat gcccatgcgc aatagacgtt
    16861 ggaaacactg gcgcaccggc ttcgcgaatt ttgtttcgat gtcctgggcc aactccgacg
    16921 gcacgcactc ctccatcccc atcagacgcg ccgcccacac ctccggcggc ttgctggccg
    16981 cgcagatcgc cagcgcccgt tcgatccatg tccgcaccgc ggctttccag gacgcctcat
    17041 ccgtcaccgt ctcgttgatc cgcatcatgg gtttgccgat gtgttcgaac agggagacga
    17101 tcaccgcacg gtagagttcc gctttcgacc ggaaatggta gttcaccagt gcgctgttca
    17161 ccgccgcctc cgcgcagatc atccgcgtgg tggcttcacg aaacccatac tcggcgaaaa
    17221 cctttgtggc cgcccgcaga atccggttcc gtgtgcggga gcgtcccgtc gttgtcccgc
    17281 ctcgtctggt ttgccgttcc atcttccgtc cctccgtgct tcaggggatt cgacgtccct
    17341 gtgccgcctc ccaaaacgca taccagtctt cacgttccaa tgtcagcgca tcagcctgcg
    17401 ccgcaagtcg caggcgatcc aaacggttgg ttccgatgat cgccatcgga tggctcggat
    17461 gcgccagaat ccacgcgtac gcgaattgat ccagcgtaac gccgccgtaa cgcacggcac
    17521 atgccgcagc cgccgcagcc aaacgaccgg ccgccggttc agccggatca aacagcctgc
    17581 cgccggccaa cggactccac gccatgggcg tcatgcccaa ccgctcgcat tgcgtcaacg
    17641 taccgtcttc catcgcctcc atgtgcagca gactgaaggc gatctggttg accgccagag
    17701 gccggtccag acaagcgttc agcagatcga cccctgccgg agcgtaattc gaaactcccg
    17761 cggcgcggat ctttccttcc tgaaccagac ggttcaaccc ctgcgccgtg tcttctgtcc
    17821 gcgtgagcca atcgggacgg tgtatcagga acagatcgat gcgttcggtc cgcaaccgct
    17881 tcagcgactg gtccagactg gcgcagagct gctcagccgt ggcgttgtaa tgcgccgtcc
    17941 gccgttcggg acaggcctcg cacggcacgt aaatgccagc cttggtgacg atctccaact
    18001 gctcccgcaa tccgggcgag agggccagcg cctcaccgag aagcgcctcc accccgtatt
    18061 tcccgtaaat ctcggctgtg tcgatcgtcg tgatgcccag ctccaaacag agatggagac
    18121 gacggttgac ctcctgcgcg gtcggcttgt ccgccaacaa gcgccacgtt ccgtaagcca
    18181 accgcgagac ctcgaacccc gccttcgtca aacgataccg ccgcatcgca ccccctcact
    18241 tgaactgcaa cagcgccgcc tcactgaggc gccagttcgc ggcacccgtg ccttccagcg
    18301 accgccacac cttgtcttcc gcgcgaaaga gaccgtaatt gaaagccact ttcataccgg
    18361 gttccggacg aatccccaac tccttgaagg ggatcgccca ctcgccgcgc cagccgcttt
    18421 tccaatcatt ccgcgtcttc ccgtacggtt tcgcggcaaa ccgcaccgcc ttgcccaaac
    18481 gttccgcctc ctccgacggc agacccgcat ccgtgacgct cacacacgcg ccgcccgcaa
    18541 atccgcggat cacggcaata cccttcgcgc cggcgatgca gagttccgcg ccgtcatcct
    18601 gcccccaacg atcgccctta ctcaacaggc cgatatcgaa gagcacggta ttcgcggcca
    18661 catagagata ctgttcgtca tacatcagct tgatgaaggc gggagcgccg ctggccagcc
    18721 agcgtgacgg gtcgcggtcg atattcacga acgaccccgc ccattcgccg tcaccgattt
    18781 caccgtccat caccggcggc tgttcggtac gcggcaccgc cacggcccaa gtccggcggg
    18841 cgggcgcggg cgcgggcggc atcgcgtcgg tgattgcaaa ggccgccgga gttgaaccac
    18901 cctgcgcgtt tccggaaaag agcacattac ccacccaaga ggttaccgcg ttgggtgccg
    18961 tcaccgtaac cttgcccggc gcgtacagcg tattgcccgt gaagacacaa tcacgagaac
    19021 ggggaaacga caacttcata tccccgtccg agacgaagac attgtcccga atcgtcacgc
    19081 cggtcgtgat gtggttgtgg gtcggcatcg gaaccccgat cgccacgttg cgctcgacca
    19141 cgttgccggt cgccccttca tcgaaataat aagcggaagc accgtacccc tgcccgatct
    19201 ccaccacatc gcgcacgaca ttgccgcgca gcaccgtgcg gctcatcgat ccgtaaatcg
    19261 ccgcaccgtc gtgtagttcg cgcatcacgc ggtaaatgag gttttcctcg atcaggtgat
    19321 ccgagccgcc gcccgtcatg ccgctgtacg gcgcatcgtg aatctcgtta cggcggatat
    19381 gaaatcccga cagcttcgaa tcccggcgca cacgcccgaa ctgcaacgcc accgcactcg
    19441 gctggtagac ccccgcgtgg tggatgtggt tgccctcgat aacactcgcg tcaccgcccg
    19501 tgcgaatgcc gcaggcgccg acatggtgga tgtggcacgc ttcgatccgg cacgcgtcca
    19561 gtgcccagga tttgataccc tggccgccga catggcagat ctccatcccg atgagtgcac
    19621 actccttcgc atgatacaat tccaccgcgc cgtcatacgc gtgagccgaa aagccgccgg
    19681 gcttcagcgg tgtggtcgtc gcctgcaccg aaaacccgcg cagcgtcaca cgctcgaccg
    19741 gcgcctgggc gctcccccgt aggcggatca ccgaggtcag ggtcggcgcg atgaccgagg
    19801 cggactccat cctctcgccc gccagcggcc agtacaccag acgtcccgcg gcacggtcca
    19861 gataccactg tccgggacgc gtcatgccct cacgcgtgtt g
//
LOCUS       DAOSLE010000038        19817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2987_length_19817_cov_7.359528, whole genome shotgun sequence.
ACCESSION   DAOSLE010000038 DAOSLE010000000
VERSION     DAOSLE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19817
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_19817_cov_7.359528"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..2222
                     /locus_tag="PK576_04255"
     CDS             174..2222
                     /locus_tag="PK576_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60835.1"
                     /translation="MVKVQMSLSIGLLATVTTGAEPKIIYTWTGEAADGNWFNPTNWS
                     GPSGYGVPGDTHDPANLQLDGAYFESASNVVGTINYTYVPGTTPHTNLFSYFYLNKTD
                     DTPLTFNVNGDLTADHFNLAEPDWYESYNTIGGGGNLTFNVNSGTFNTGLGKVSVNLA
                     DLYVASGATLSGFVRPSGNMTITVKNQARIQGRLGYEPFANDYATRNNVVTIADGELV
                     IDGGVLYPDYFSCGKYGMLSVTNGGMVLTRFGGSGIAMNGYHNKYYTNRITVTSGSVT
                     NVGIFTIGGVNTYGVGTPWPGRSIVTVHGGVWFQGGATCLGDGRLGFLTLSGGTFETP
                     ADFYVGGGRSVDWDVPIAGVEYSGTLNVDGGTMTVCNPEAYVLQIATLAGKGSSSDLY
                     FGQTGWDRTYRGQRVVFDALKSDGTGLESNRVYFVADINTASTIQKTCFSVETNLTLT
                     KTGVTGTPYNSGTGNNIYHTFPAQLRVGNWMTFKTGTLLNTGTLNLASGSLTADELIA
                     TNGTASILSFAGGTLTVKQFTDINNGFATVFGDGESSALLELVAGTHHFADGLVITNN
                     ATLAIGGTNAIGSVGLMGGLAFARGAALDVDFSAATNDWLEVGGAVALADEVTLTLRM
                     SDDTARSPVTILKASEGIEGSAGAWPSATVNNIKYRAVVVGDELRLEKVAQGTMVNIL
                     "
     gene            2233..3186
                     /locus_tag="PK576_04260"
     CDS             2233..3186
                     /locus_tag="PK576_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE60836.1"
                     /translation="MQRRDFMKTAALTAAGGVVSAARAHEGGKGAKVCLHLFSKPLQW
                     MGVDRLVEVVAEAGYGGIDLTVRPGGHVKPEKAGTELPRVVEAARRQGLKIRMMTTSL
                     TDPEDPAAERLLKTAAQCGIRIYRMGYLRYAGGRPMGEELERLRRVLAAFEQLNRSCG
                     ISGGYQNHRAWEERLFGGVLWDLHAALKGLDPQWIGSQYDVHHAVAETGGSWVAGMRL
                     IAPHITSTCLKDYIWPAADKQRHQGVFAGEGLVPWKRYYELVKELRIGGPASVHCEWE
                     LFTKAEQALTEPERCAIAVRKLKRDSEFFTAQYANCGLTVE"
     gene            3420..4715
                     /locus_tag="PK576_04265"
     CDS             3420..4715
                     /locus_tag="PK576_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine-DL-glutamate epimerase"
                     /protein_id="HOE60837.1"
                     /translation="MKKTITVADTGCEFEREPLLRPFGFKGGYLTNIWQSAAMVQSTS
                     GQRGIGLCTHSTLWSDAAVFAANSEAGGNALMFAMTAHALRVLKGRSFTDPVALNDEL
                     FKEVYAYGKAITGNPRLRQTFALNALVGVDNAIWLLYAKENGITTFDGLIPAAYRPAL
                     AHRHPKAASIPLMAYAIPISEIKAAVADGSFFMKIKIGQPGTQEEMLAKDMARISEIH
                     KALDGCRTPYTKSGKLPYYFDANGRYEKKETLLRLIDHLKKIGAYDQVAVVEEPFDEQ
                     YECDVHDIPLRLVADESAHTERDAVTRIEMGYRAIALKAIAKTLSMTMKIAQAAHERN
                     VPCFCADLTVNPILVEWNKNVAARLGSFPGLEGLGLVETNGHQNYKNWTVMRSYHPYP
                     DAPWSKTDRGVFHLDADYYAKSGGIFAPLPHYEAMFPKT"
     gene            4732..6450
                     /locus_tag="PK576_04270"
     CDS             4732..6450
                     /locus_tag="PK576_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60838.1"
                     /translation="MTGGVRCGGGIGIALALSVTMGWGAPQTSLTAGRMRVAATADRI
                     SVSLEGVERFAFPARVAVDTEEGGADRDLFEPAFRQEAGRCVWTSRTTNWEKKEVTLS
                     VEGGAAVLRVTVQGKGKLGKLRFFPQTDRTGPLAYEVSRYLLPVALGGSRCVPQWRNT
                     MESAGIELGYMTPPLLAFPFVGTFRGSCAVGLAPKPGAYHVDHFRCEFAAFREGLFST
                     DFLGYTEVQGEYELPALIMTAGEDAFDALSAYSEWLYREGGCRRIERTQVPRWWLGPF
                     FCGWGEQGYHSPTNVYAGANQKAYAAMSERLDAFGLKPSAIIVDDKWQRRYGELLPDP
                     VKWPDMRAFVEAEHAKGRRVLLWLKAWDNEGLSSEECVKCLCTPYGADPTSPTYRRRI
                     QETMRRLLSAEPGCFNCDGFKIDFANCMPLGRNLATHERNVYGIELLKRLMTLFYESA
                     KAVKPDCLINNSCCHPYFAEVTDQCRLHDYNGQLRSLWEVREFRARLFKTAFPGISVD
                     TDDPHSTSHAEMMNYLRRAPDLGVPDLYQLHRRIRDEDLREIAGIWEAYSARLEKSVD
                     SKGGKR"
     gene            6495..10217
                     /locus_tag="PK576_04275"
     CDS             6495..10217
                     /locus_tag="PK576_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOE60839.1"
                     /translation="MFRVLLAGFCTAASLRAAEPAVVWVSQPVAPGEAVVVYGGPWTN
                     VTGVMLKGPSELTVTPVRATDDCVTFVYPKTWPLAAFDARVVGGNGTAELRVNAPEVW
                     WVQGDAGMRATPGGWLRVFGRCIGYDGKASLTLRDHTGKATTLAAGESGPYALRVDLP
                     QSLQAGTFEITLRNGLDSRAVPVGAVEILAPREPWPERVFDITAYGAVANDFNDDTAA
                     IRAALAALEQAGGGVLYVPRGRFGIQGELSLPPKTLLRGAGMALSQLYWLDEDNPVGA
                     LVSGTRDFGIEEIFLAAGNIDQGILVVRPKEGETWINENILLRRVRTRFLHTDTVPHE
                     ESFRRLSGGQSFSINGTYVRVIGCDFYSSKGSSGLGGRYLEVRDNRFDGESCGYTGGR
                     FAIFENNNHERQSMSFGNGSRCVYFKGNQLGGIYGNGDRETFTFDGGDMGYIDALQGV
                     GPASVTLKPKPLRRAAERWIDEPLYIAGGRGAGQLRYIRSIAGQEVKLDRPWDILPAT
                     NVLAVIAPVRHRLLILENQVRDGNPFAMYGSGAEMVLGGNKMMRNSGLHAHGMWKGVS
                     EPCWFVEFVENELLEGNAVRGPFSFQVPAADGWIGFFDRGIARPYSYPQNRVGIMRRN
                     VLHNNAYLASHGRVKNLLVENNLIRDADRGIVIAESVEDAVLLRNRFERVLRPYAVGG
                     QTRIGAADSLNAGLSAVEAELGSAVPEAWPVYRKEAEALAAMGLPQEEEAKWVSVMLL
                     RAVRALSESLRGRALSPATVASLMGIDLAQKSIWQFTRVAPGNPRKFAVGGPLLPQWG
                     LPAALTGSAGTFEGWTVRVDTPKPLEPGRPVVCDLYLASTAGPLPPFTLPVRYTLAGQ
                     GWTLAFEERYGWDQLPIQSFLAAGPFANVSGKAIDTTVHPPEIELDMKASYMTRDGNR
                     PWVPVTPNAKGEVDLSQVFKSNELATAHLVAVVRAARGIRVNVPPGGHNALLFVNGER
                     IGSSARRDAPRCVDLRQGDNLFHLISSHTSGAWPIRLTLTMIDPVRPGDLTVVPTDQI
                     ASSPALTASANAVPEGKGLPDSMGVDWKLVVRDDFNRMRLGDQWRGLAVSWMPQGVNI
                     LEGAMRPEFGFGSVLFHREVALPVRIEWKVRRDGPDSKWTGVSLAPKGLSYRTFWYTL
                     QGRGYFLSLGWHDAKNNRVLRNTETVVLDEKGPMLEPGVWHHVVAQFVPPHCRMYLDG
                     KQVLAFEDPDFLPGLNEIGLYSNGHAFDDIRIYTGR"
     gene            10301..13987
                     /locus_tag="PK576_04280"
     CDS             10301..13987
                     /locus_tag="PK576_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOE60840.1"
                     /translation="MRWMRLGHAAAGFCAAISVGAGEPAVVWLSQPVAPGEAVMVYGG
                     PWTNVTAVTLDGPKQESVVPLKVTDDCVTFEYPHAWPLAAFDVRIRGGNGEAVQRVNA
                     PDVWWVQGDAGKNVTAGGWLRVFGRCIGYDGKAVLEWRDSAGTSRSLPAKSGDRYAVR
                     VDIDPAFPAGVYEVSLLNGLDSRPFRIGKVEVVARRETWPERVFDITAYGAVANDFND
                     DTAAIRAALAALEQAGGGVLYVPRGRFGVRGELSLPPKTLLRGAGMALSQLYWLDEDN
                     PVGALVSGTRDFGIEEIFLVAGNIDGGIVAKRPQAGESWTNENILLRRVRTRFLHTDT
                     TPIAVSYHRSRSSAKPLFVNGTFVRIQECDFYFSKGNSALHGQYLDIRDNRFVGPECG
                     YLSGKQVIFEGNDHEYRGLSFGNGSHNVFFKSNRLGGAYGEGDRETFTFDGGDPAYSD
                     KAVACDGRHVTLKPGSWRHGEAHWIGVPLYIIGGRGAGQMRWVTGIRERTVELDRPWD
                     IQPDDQSHFVLAQMRSQLLFLDNTDRDGNPFALYGTASDVVIAGNSLERTGGLHAHGM
                     FKSVPEPSWFVQFFGNRIVEGNSVRGPFSYVVPASDSWLGFFDRGIRRPLTYPQNRVG
                     VMRGNTLENNAFLAAHGRVKNMLMENNLVREADCGIVIESRVQDAIVRGNRFENVLRP
                     LVLHDQVLCRASERLVAGLSAVDALASDVVPSAAWRELADAAVSLSRRRLPDAEEVTE
                     TRTLLARAASLLAARVGDRKLSAAVVDALFGLDLTQKSDALFSRLQSGKEVKVPLGGT
                     YPVWAVPATLSAAVEGFEGWGVRVASPVKLVPGGQCGFALHVTRPDDRPQTFALPVRF
                     TLDGEGWSVTFTETYRQDTLNVTDFLVAGPFRNASGKPIDSAVHPPESRLDVTESYDT
                     LDGRKPWRAVRAAKPDGTVDLNTCFRNTELATAHAVAIVYAARPLRVRMQYGSNQNTL
                     ARVNGEAVGSSARRDAYNGRPVDLREGENVIHLISTHATGAWPLKVRLTAVDATRPGE
                     LRVVSSDRLFQSDRLKAEGRRGVVGEPFTDGEGRVWRLSHEDTFNRLRVGLQYEGRDP
                     IEWRDHSVRILDGRLVGAGRITLNLRLSAPLRVEYDVPVVNGRANTGLTLAARGASAV
                     VRDWGEAMKQKQVVTLNNVLGGLTGETARVVAVFDTKGSTLFISGKKMREVPDTEWVR
                     GLDELTLSGTLDNLRIHTGERGKP"
     gene            14315..15730
                     /locus_tag="PK576_04285"
     CDS             14315..15730
                     /locus_tag="PK576_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013464554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60841.1"
                     /translation="MSIQASRRNFLKLSTLAGSGVMAAKAVAPLTAQAQSAAAGQTVM
                     GFRAPKLERVRVGFIGLGRGSGHLGDMLKIEGAEVTALCDIHQPTLDRALKRVADAGR
                     PKPATFSDGPEAYKKMLERDDVDIVYIATPWELHTPQALDTMRAGKHAFLEVPAAITT
                     EECWALVDTAEKTRRHCMMLENVNYGREELMVLNMVRQGLFGELLHGEAAYIHELRGQ
                     MNQVEHGTGSWRTLHYARRNGNLYPTHGLGPIAQYMGINQGDRFDYLSSVSSPALGRP
                     LYAKERFPADHKWNQIKGWRCGDINTTIIKTVKGRSIMVQWDETSPRPYSRHNLIQGT
                     RGTFAGFPNRIALDYRKEDLPEPVRKALGDKPRTNYHGWDQNMAPWFEAYDHPLWKRM
                     GAEAKRVGGHGGMDFIMNWRVIYCLRNGEPLDQTVYDAAAWSAIGPLSERSVTERSRS
                     VDIPDFTRGVWERTQPFGIVS"
     gene            complement(15745..17013)
                     /locus_tag="PK576_04290"
     CDS             complement(15745..17013)
                     /locus_tag="PK576_04290"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HOE60842.1"
                     /translation="MRKDTHRKFALNFLLFAILTAVSAFGWGDAHSVITRAALSTLPS
                     SDAALLGNEREPLSAGYCLIPDWVYKKDGNERFVWDFMGTESYSGRFENFHLPWNAAS
                     NYEMLEYYIRHAVASFKTNDAASAARHLGVLAHMLEDWTCPAHSSVGDNMYRLLGQYM
                     PAPAGKEHMLLHAHLESGGFDLSGYTHTPVALGATTEEVAFRLLSRAQAGTVFARGRV
                     LDIVRAVYAEDKQASAAAQRPSAILGADLVADAFRSVCALARGEAPPPAPIALTPYWP
                     NEAPSLFYPQAAFFSSPYWGHPQRDMILRDGKDPVPLALQISGERTEVKGLGIGVKRI
                     SWFLPGNLFSRIMLRVGLHADLGTDGQVTFRVFGNDAELHKTELRGTAQSVPIDLPIT
                     GVTNLAFLASPINRSKANYVVLADPMLIPK"
     gene            complement(17169..17852)
                     /gene="cmk"
                     /locus_tag="PK576_04295"
     CDS             complement(17169..17852)
                     /gene="cmk"
                     /locus_tag="PK576_04295"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOE60843.1"
                     /translation="MSRVIAIDGPSGAGKSSVSRRVGQHLGFLHVDSGALYRIMTWQC
                     LQNGIDTSDPEAVAAFAQKVEVECEPRDGAIIYRVQGQEPGDAIRTPEINRHASPVAT
                     VKAVRDRVTGWLRGMTRFGDLIVEGRDITTAVFPDSPARFYLVASAEARAERRRKEEV
                     EKGIANQSAEEVKRSLLARDAIDSTRQHAPLRKADGALEIDSTYLTLEQVVQMVLDAL
                     PTAWKGGDA"
     gene            complement(18070..18198)
                     /locus_tag="PK576_04300"
     CDS             complement(18070..18198)
                     /locus_tag="PK576_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60844.1"
                     /translation="MTLAKLTSKDQLTLRPLSAVHEKMEQLNITEADIGKAVAWSQ"
     gene            complement(18308..>19817)
                     /gene="carB"
                     /locus_tag="PK576_04305"
     CDS             complement(18308..>19817)
                     /gene="carB"
                     /locus_tag="PK576_04305"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOE60845.1"
                     /translation="QGIEFDYCCVHAAFAMRDAGYETIMVNCNPESVSTDYDTSDKLY
                     FEPVTLEDVLQIYENEKPEGVIVQFGGQTPLNIARELADAGVKILGTSIDSIDTAEDR
                     DLFRKMMERLAIPMPESGMAANIDEAIRVAGSIGYPIMIRPSFVLGGRAMEVIYDEEM
                     LREYVAKAVGVTPDRPLLLDRFLKEALECEADALSDGREVYIPAVMEHIELAGVHSGD
                     SACVLPPVSIPDRHLDTILAYTRKIAQELQVVGLMNMQYAIENDTVYVIEANPRASRT
                     VPLVSKVCDTQMARIATRLMLGASIADLDLRPKVIPHYGAKEAVFPFDKFPEVDPVLG
                     PEMRSTGEVLGMASTFGLAYYKSQEAANARLPTEEGTLLVSLSEKPDSAAKVISAFVK
                     LGFRIIGTEGTILWLKEHGVPGEVVQKINEGRPNVLDAIVNRQVCLVINTPSARRDAR
                     ADDASIRKAAIKYKVPYLTTLAAAAAAAQGIRSARDSAGGVKSIQAYHANIR"
BASE COUNT         3739 a         5386 c         6829 g         3863 t
ORIGIN      
        1 gcctcgctgg tgctgctcaa gaaaccgaag ggtatggtga tcagcgtgtt gtaactgagg
       61 ctggcgtgtg accccccgtc tgtctgtcca gtccgccccg atgatattca ggctccacgg
      121 gccgaaggag cgcatgtgcg aaaggaaaaa ccatgaaacg gatgcaaaga tggatggtaa
      181 aagttcaaat gtccctctcg attggtttac tggcgacggt gacgactgga gcggagccca
      241 aaatcatcta cacctggaca ggggaggctg ccgatggaaa ttggttcaac cccacgaact
      301 ggagtgggcc ttccggatat ggggttcccg gcgacacgca tgatccggct aacctgcaac
      361 tcgacggagc ctatttcgag tcggcgtcga acgtggtcgg cacgatcaac tacacgtatg
      421 tgccgggtac caccccgcac acgaatctgt tctcatattt ctatctcaac aaaacggacg
      481 atacgccatt gacgttcaac gtcaacggcg atctgacggc ggatcatttc aatttagccg
      541 aacccgattg gtatgagagc tataacacga tcggcggcgg gggtaatctg acgttcaacg
      601 tcaattcagg gacgttcaac actggccttg gaaaggttag tgtgaacctt gctgatctct
      661 atgtggcgag cggtgcgacc ctctcggggt ttgtgcgtcc tagtgggaac atgacgatca
      721 ccgtgaagaa tcaggcgagg atacaaggac ggctgggcta cgaaccgttt gcgaacgatt
      781 acgcaacccg taacaatgtc gtcacgattg ccgacggtga actggtgatc gacggcgggg
      841 tgctttatcc cgattatttc agttgcggca agtatgggat gttgtcggtc acgaacggtg
      901 gcatggtcct tacccgtttc ggcggttctg gaattgccat gaacggatac cacaataagt
      961 attacacgaa ccgcatcacg gtgaccagcg ggtctgtaac caatgtcgga atcttcacga
     1021 tcggtggggt caacacctat ggggttggga cgccatggcc gggaagatct attgtgactg
     1081 ttcacggggg cgtctggttt caaggcggag caacctgtct cggtgacggc cgacttggtt
     1141 tcttgaccct gtcaggcggg acgtttgaga cgcctgccga tttctatgtg ggaggaggac
     1201 gaagtgtgga ttgggatgtg ccgatcgccg gagtggaata ttcgggaacg ctaaacgtgg
     1261 acggcgggac catgaccgtg tgcaatccgg aggcgtatgt gctccagatc gcgacacttg
     1321 caggcaaggg gtcaagctcc gacctctatt tcggtcagac gggatgggac cgtacctatc
     1381 gcggtcagcg ggtggtgttt gacgcgctga aatccgacgg caccggtttg gagtcaaacc
     1441 gcgtgtattt tgtggcggac atcaataccg cctctacgat tcagaagacc tgcttcagcg
     1501 tcgagaccaa tctgacgctc actaaaaccg gtgtcacggg aacaccgtac aattcaggca
     1561 cgggcaacaa tatctatcat acattcccgg cgcagttgcg tgtcggcaac tggatgacgt
     1621 tcaagaccgg aaccctgctt aataccggca cgctgaatct ggcgagcggt tcgctgacgg
     1681 cggacgagct gatcgccacc aacggcacgg cgagcatcct gagttttgcg ggcggtacgc
     1741 tgacggtcaa gcagtttacc gacatcaaca acggtttcgc gacggtcttc ggcgacggcg
     1801 agtcgtcggc gctgcttgag ctggttgccg gcacgcacca ctttgccgac ggtctggtga
     1861 tcacgaacaa cgccacgctg gcgatcggcg ggaccaatgc gatcggctct gtaggcttga
     1921 tgggtggcct cgcctttgcg cgcggggcgg cactcgatgt ggacttcagc gcggcgacca
     1981 acgactggct ggaggtgggg ggcgctgtgg ctctggcgga tgaggtgacc ctgaccttgc
     2041 gcatgtcgga tgatacggcg cgttcgccgg tgacgatcct gaaggcgtcc gaagggatcg
     2101 agggttcggc tggcgcatgg ccgtctgcca cggtcaacaa cattaagtac cgggcggttg
     2161 tggttggcga tgagttgagg cttgagaagg tcgcgcaggg cacgatggtg aatatcctgt
     2221 aggggaggcg gaatgcagcg cagggatttt atgaagacag cggcgctgac agcggccggg
     2281 ggggtggttt ctgctgcgcg tgcgcacgag ggcgggaagg gcgcgaaggt gtgtttgcat
     2341 ctcttttcaa agccgctcca atggatgggg gtggaccggc tggttgaagt ggtggctgag
     2401 gccggttatg gcgggatcga cctgacagtg aggccgggtg gtcatgtgaa acccgaaaag
     2461 gccgggacgg aactgccccg cgtagtggag gccgcgcgca ggcaggggct caagatcagg
     2521 atgatgacca cctccctgac cgacccggaa gatccggcgg ccgagcgttt gctgaagacg
     2581 gcggcacaat gcgggatccg gatttaccgc atggggtatt tgcgttatgc cggcgggcgg
     2641 ccgatggggg aggagttgga gcggttgcgt cgcgtgttgg cggctttcga gcaactgaac
     2701 cggtcatgcg ggatcagcgg tggttaccag aatcaccgcg cgtgggagga acgcttgttc
     2761 ggcggcgtat tgtgggacct gcacgcggcg ctcaaggggc ttgacccgca atggatcggt
     2821 agccaatatg atgtgcatca tgcggtggcg gagacgggcg ggtcgtgggt ggcggggatg
     2881 cggctcatcg cgccgcacat cacgagtacc tgtctcaagg actatatctg gccagcggcc
     2941 gacaagcaac gtcatcaagg cgtgttcgca ggcgaggggc tggtgccgtg gaagcggtat
     3001 tatgaactgg tgaaggaact ccggattggg gggccggcat cggttcactg tgagtgggag
     3061 ctgttcacaa aagcggagca ggcgttgacg gaaccggaac ggtgcgcgat cgcggtgcgc
     3121 aagctgaagc gcgacagcga gttctttacg gcgcagtatg cgaattgcgg gctgacggtg
     3181 gaatgagcca cggatggggt acggatggaa cgcggatttg gagtgccgga tcaacgtgtt
     3241 gttcaaaggc gttctcgtgg gcaagggtta tgcggaacaa tccgcgttcc atccgtgtcc
     3301 ataacaacag aaaggatgtc atgaaacgca gggattttat gaaggcggcg gcactgacgg
     3361 cgggagtaac actggcaaac ggatgtgtct cggcgggcgc gtcaaacgga gaaaagacga
     3421 tgaaaaaaac gatcaccgtg gcggacacgg ggtgcgagtt cgagcgcgag ccgctactgc
     3481 ggcctttcgg attcaagggg gggtacctga ccaacatctg gcaaagcgcc gcgatggtgc
     3541 aaagcacatc cggtcagcgt gggatcgggc tctgcacaca cagcaccttg tggagcgatg
     3601 ccgcggtgtt tgcggcgaat tccgaggcgg gcgggaacgc gctgatgttc gccatgaccg
     3661 cgcacgcgct acgcgtgttg aagggacggt cgttcaccga tcccgtggcg ctcaacgacg
     3721 agcttttcaa agaggtgtac gcatacggca aggccatcac cggcaacccc cggttgcgtc
     3781 agacctttgc gttgaacgcg ctggtcggcg tggacaacgc gatttggctg ctctacgcga
     3841 aagaaaacgg gatcacgacg tttgacggac tgatcccggc ggcataccgg cccgcgctgg
     3901 cccaccgcca tcccaaggcg gcctcgattc cgctgatggc ctatgcgatt ccgatcagcg
     3961 agatcaaggc ggcggtggcg gacggttcct tcttcatgaa aatcaagatc ggtcagcccg
     4021 gcacacagga ggagatgctg gccaaggaca tggcgcggat cagtgagatc cacaaggcgc
     4081 tggacggttg ccgcacacct tacacgaaga gcggcaaact gccgtactat ttcgacgcca
     4141 acggtcggta cgagaaaaag gaaacgctgc tgcgcctgat cgatcatctc aagaagatcg
     4201 gggcgtacga tcaagttgcg gtggtggaag agccgtttga cgagcaatac gagtgcgatg
     4261 tccacgatat tccgctgcgg ctggtcgcgg acgagagcgc gcatacggaa cgggacgcgg
     4321 tcacgcgcat cgagatgggc tatcgggcca tcgcgctgaa ggcgatcgcc aagacgctca
     4381 gcatgacgat gaaaatcgcg caggcggcac acgagcggaa tgtgccgtgc ttctgcgcgg
     4441 acctgacggt gaacccgatc ctcgtggagt ggaataaaaa cgtggcggcg cggctcggtt
     4501 cgttcccggg actggaaggc ctcggactgg tggaaaccaa cggccatcag aactataaga
     4561 actggacggt catgcgcagc tatcacccgt atccggacgc gccttggtcc aaaaccgacc
     4621 gcggcgtgtt tcatcttgat gcggactatt acgcgaagag cggcgggatt ttcgcgcccc
     4681 tgccgcacta cgaggcgatg ttccccaaaa cgtgacggaa ggaagtgtgg aatgacgggt
     4741 ggggttagat gtgggggagg gatcgggata gcgctcgcgc tgtctgtgac aatggggtgg
     4801 ggagcgccgc aaacctcgct gacggcggga cggatgcggg tggctgcgac ggccgaccgg
     4861 atttccgttt ccctggaggg ggtggaacgg ttcgcgtttc ccgcgcgcgt ggcggtggat
     4921 acggaagagg gcggggcgga ccgggatctg tttgagcccg ccttccggca ggaggcgggg
     4981 cgctgcgtgt ggacgtcgcg cacaaccaac tgggaaaaga aagaggtgac gctttcggtt
     5041 gagggaggcg cggcagtgct acgcgtcacc gtgcagggca aggggaaact gggaaagctt
     5101 cgctttttcc cccagaccga tcggaccggt ccgctcgcct acgaggtgtc gcgctatctg
     5161 ttgcctgtcg ccttgggcgg ttcccggtgc gtgccgcagt ggcgcaacac gatggagagc
     5221 gccgggatcg agttagggta catgacgccg cccctgttgg cgttcccgtt cgtcggaacg
     5281 ttccggggta gttgcgcggt cgggctggct cccaagcccg gcgcgtatca cgtcgaccat
     5341 ttccgctgcg agtttgccgc attccgggag gggttgttct ccacggactt tctcggttat
     5401 acggaggtgc agggggagta tgagcttccc gcattgatca tgacggctgg cgaggatgcg
     5461 ttcgatgcgt tgtcagccta ctccgagtgg ctttaccgtg aaggcgggtg ccgcaggata
     5521 gagcggacgc aggtgccgcg ctggtggctg gggccgttct tctgcggttg gggagagcag
     5581 gggtaccatt cgccaacgaa tgtgtacgcg ggtgccaatc aaaaggcgta tgccgcaatg
     5641 tccgaacggc tcgacgcctt tggtctgaaa ccgagcgcga tcattgtgga tgacaagtgg
     5701 cagaggcggt atggcgagct gttgcccgat ccggtgaagt ggccggacat gcgggctttc
     5761 gttgaggcag agcacgccaa gggccgcagg gtgctgcttt ggctcaaggc atgggacaac
     5821 gaagggctct cttccgaaga gtgcgtgaaa tgcctgtgca cgccgtacgg agcggatccg
     5881 acttcgccga cctaccgcag acgcattcag gaaaccatgc gccgcctgct ctcggcggaa
     5941 cccggttgct ttaactgcga cgggtttaaa atcgacttcg ccaactgcat gccgctggga
     6001 cggaatctgg ccacgcacga acgaaacgtc tacggcatcg aactgctgaa gcggctgatg
     6061 acgctgttct acgagtctgc caaggcggtc aagcccgact gcctgatcaa caacagttgt
     6121 tgccatccct atttcgccga ggtcaccgac cagtgccgcc tgcacgacta caacgggcaa
     6181 ctgcggagtt tgtgggaagt ccgcgaattc cgggcgcggc tttttaaaac cgcttttccg
     6241 gggatcagcg tggacacgga tgatccgcac tccacgtcac acgccgagat gatgaattac
     6301 ctgcggcgcg cgccggatct gggtgtgccg gatctctatc aattgcaccg gcgcatccgg
     6361 gacgaggatc tgcgggagat cgccgggatt tgggaagcgt attccgcgcg tttggagaag
     6421 tccgtcgatt cgaagggcgg gaagcggtga aaaaaccgta aaaatcaaaa gggggggcgg
     6481 gaatgggtaa gggcatgttt cgtgtactct tggcggggtt ctgtacggcg gcgtcgcttc
     6541 gggccgccga gccggccgtg gtgtgggttt cccagccggt ggcgccggga gaggcggtgg
     6601 tggtttacgg cgggccgtgg acgaacgtga cgggtgtgat gttgaagggg ccctcggaac
     6661 tgacggtcac gcccgtgcgg gccacggacg attgtgtgac gttcgtgtat cctaaaacgt
     6721 ggccgctggc ggcattcgat gcgcgggtag tgggcgggaa cggaacggcg gaattgcggg
     6781 tgaacgcgcc tgaggtgtgg tgggtgcagg gagatgccgg catgcgggcg acacccggcg
     6841 gatggttgcg tgtgttcggc cgctgtatcg gctacgatgg caaggcctct ctgacgcttc
     6901 gcgaccatac gggaaaagca accacattgg ctgcgggaga aagcggcccc tatgccctgc
     6961 gtgtcgacct tccccagagt cttcaggccg gtacgttcga gatcaccctc cggaacggac
     7021 tggacagtcg tgcggtgccg gtcggggcgg tcgagattct cgcgccgcgc gagccttggc
     7081 cggaacgggt gtttgacatc accgcgtacg gcgcggtggc gaatgatttc aatgacgata
     7141 ccgccgcgat ccgggccgcg ctggctgcgc tggagcaggc cggcggcggt gtgctgtacg
     7201 tgccgcgcgg ccgtttcggg atacagggcg agttgtcgct gccgcccaag accctgttgc
     7261 gcggggcggg catggcgctc agccaactct actggctgga cgaggacaat ccggttggcg
     7321 cactggtgag cggcacgcgg gatttcggga tcgaggagat ttttctggcc gcaggcaaca
     7381 tcgaccaagg cattctggtg gtccgcccga aagagggtga gacctggatc aacgagaata
     7441 tcctgctgcg ccgcgtgcgc acacggtttt tgcacaccga caccgtgccg cacgaggagt
     7501 ccttccgtcg cttgagcggc gggcagtctt ttagcattaa cgggacgtat gtgcgggtca
     7561 tcgggtgtga cttctactcg tccaaaggat cgtcaggact gggaggccgt tatctcgagg
     7621 tgcgggataa ccggtttgac ggcgagagtt gcggttacac tggcggacgg ttcgcgatct
     7681 ttgagaacaa caaccatgag cgccagagca tgagtttcgg caacggcagc cgttgcgtct
     7741 atttcaaagg caaccaactc ggcgggatct acggcaacgg ggatcgcgag acctttacct
     7801 tcgatggcgg tgacatgggg tatatcgacg cgcttcaggg ggtcggcccg gcgagtgtca
     7861 cgctcaagcc caagccgctg cgccgcgctg cggaacggtg gattgacgag cccctgtaca
     7921 ttgccggcgg gcggggtgcg ggccagttgc ggtacatccg ctccatcgcg gggcaggagg
     7981 tcaagcttga ccggccgtgg gatatactgc cggccacgaa cgtgctcgcg gttatcgcgc
     8041 cggtgcgcca ccgtctgctg attctcgaaa accaggtgcg tgacggcaat cccttcgcca
     8101 tgtatggcag cggtgcggag atggtgttgg gcggtaacaa gatgatgcgt aacagcgggc
     8161 tgcatgcgca cggcatgtgg aaaggcgttt ccgagccctg ttggttcgtt gagtttgttg
     8221 agaacgaatt gttggagggc aatgcggtgc ggggtccctt ctcgtttcag gtgccggccg
     8281 ctgatggctg gatcgggttt ttcgaccggg gcatcgcgcg gccgtattcc tatccgcaaa
     8341 accgggtcgg catcatgcgc cgcaacgtgc tccacaataa cgcgtatctg gccagccacg
     8401 gccgcgttaa aaacctgctg gtggagaaca acctgatccg ggacgcggac cgggggatcg
     8461 tgatcgccga aagtgtggag gatgcggttt tgctgcgcaa ccggttcgag cgggtcctgc
     8521 gcccgtatgc ggttggcggc cagacgcgga tcggagcggc tgacagcctg aacgccggat
     8581 tgtcggcggt ggaggcggag ttgggcagcg cggtgccgga ggcgtggccc gtctaccgta
     8641 aggaggccga ggcccttgcg gcgatgggtc tgccgcaaga agaggaggcg aaatgggtct
     8701 ccgtgatgtt gttacgcgcg gtgcgggcgt tgtcggaaag cctgcggggc agggcgctct
     8761 cccccgcgac ggtggcgtcg ctgatgggga tcgatctcgc ccaaaaatcg atttggcagt
     8821 tcacccgggt ggccccgggc aacccgagga aattcgctgt gggcggtccg ctcctgccgc
     8881 aatggggttt gcctgccgca ctgaccggaa gtgccgggac gtttgaaggt tggacggtcc
     8941 gggtcgacac gccgaagccg ctggaacccg gcaggccggt ggtctgcgat ctgtacctgg
     9001 cgagcaccgc cgggccgttg ccgcccttca cgctgccggt ccgatacacg cttgccggcc
     9061 aaggatggac gctcgcgttc gaggagcgtt acggttggga tcagcttccg atccagtctt
     9121 ttttggctgc gggaccgttt gccaacgtga gcggcaaggc gatcgacacg acggtgcatc
     9181 cgccagaaat cgagttggac atgaaggcgt cgtacatgac gcgtgacggg aatcgcccgt
     9241 gggtgccggt cacgcccaat gccaagggcg aggtggatct ctcgcaggtg ttcaagtcca
     9301 acgaactcgc cacggcgcat ctggtggcag tggtgcgcgc ggcgcgcggg atacgcgtga
     9361 atgtgccgcc gggagggcac aacgcattgc tcttcgtcaa cggcgaacgc attgggtcca
     9421 gcgcacggcg tgacgcgccg cgctgtgtgg atctgcgtca aggggacaat cttttccacc
     9481 tcatctcgtc gcacacctcg ggtgcgtggc cgatccgcct caccctcacc atgatcgatc
     9541 cggtgcgtcc cggtgatctg acggttgtgc cgaccgatca gatcgcgtcg tcgcccgcgc
     9601 tgaccgcaag cgcgaacgcc gtgccggagg gcaagggatt gccggattca atgggcgtcg
     9661 attggaaact cgtggtccgg gacgatttta accggatgcg gctaggggat cagtggcgcg
     9721 ggctcgccgt cagttggatg ccgcagggcg tgaacattct ggaaggcgcg atgcgtccgg
     9781 agttcgggtt cggttcggtg cttttccatc gcgaggtcgc gttgccggtg cggatcgagt
     9841 ggaaggtccg ccgggacggc ccggattcca agtggaccgg tgtgtcgctg gcaccgaagg
     9901 ggctctcgta ccggacgttc tggtacaccc ttcagggccg cgggtatttt ctgtcgcttg
     9961 gatggcacga cgcgaagaac aatcgggttc tgcggaatac cgagacggtg gtgctggatg
    10021 agaaaggccc gatgctcgag cccggggtct ggcatcatgt ggtggcgcag ttcgtgccgc
    10081 cgcactgccg catgtatctg gacggcaaac aggtgttggc gttcgaggat ccggacttcc
    10141 tgccggggtt gaatgaaatc ggcctgtaca gtaacgggca cgcctttgac gacattcgta
    10201 tctacaccgg aagatgattg gggcggaaga acgaagtgcg gggtgcgaag gaagatcgct
    10261 ctccgtggcg tgaatggcat gtgatgaaag gggatcggat atgcgctgga tgcgtttggg
    10321 tcatgcggcg gcggggttct gtgcggcaat ttcggttggg gccggagaac cggcggtggt
    10381 ctggctgtcg cagccggtgg cgcccggtga ggcggtgatg gtttacggcg gtccgtggac
    10441 caacgtgacc gcggtgacgc tggacggacc caagcaggaa agcgtggttc cgttgaaggt
    10501 gacagatgat tgcgtcacct tcgagtatcc gcacgcgtgg ccgctggcgg cgttcgacgt
    10561 gcggatccgc ggcggtaacg gcgaggcggt gcagcgggtg aacgcaccgg acgtttggtg
    10621 ggtacagggt gatgccggga aaaacgtgac cgcaggcggt tggctgcgtg tgttcggacg
    10681 ttgtatcgga tatgatggaa aagccgtact ggaatggcgg gacagtgcgg ggacgtcgcg
    10741 gagcctgccg gcgaaaagcg gggaccggta cgcggtgcgg gtcgacatcg atccggcttt
    10801 tccggccggc gtttatgagg tttcgctgtt gaacgggctg gacagccgtc cttttcggat
    10861 cggcaaagtc gaagtggtgg cgcggcgcga aacgtggccg gaacgggtgt ttgacatcac
    10921 cgcgtacggt gcggtggcga atgacttcaa tgacgacact gccgcgatcc gggccgcgtt
    10981 ggcggcgttg gaacaggcgg gcggcggtgt gctgtacgtg ccgcgcggcc gtttcggggt
    11041 acggggcgag ttgtctctgc cgcccaagac cctgctgcgc ggggcgggca tggcgctcag
    11101 ccaactctac tggctggacg aggacaatcc ggttggcgca ctggtgagcg gcacgcggga
    11161 tttcgggatt gaggagattt ttctggtcgc aggcaatatc gatggcggca ttgtggcgaa
    11221 acggccccag gcaggggagt catggaccaa cgaaaacatt ttgctgcgcc gtgtccgcac
    11281 gcggtttctg cacacggaca ccacgccgat tgcggtcagt taccatcgtt cccgttcgag
    11341 tgcgaaaccg ctgtttgtca acgggacgtt cgtccggatt caggagtgtg acttttattt
    11401 ttcaaaaggg aactctgctt tgcatggcca atatttggac atccgcgaca atcggtttgt
    11461 ggggccggag tgcggatatc tgagcggtaa acaggtcatt tttgagggga atgaccatga
    11521 gtaccgtggc ctgagctttg ggaatggcag tcacaacgtg tttttcaaga gcaaccggtt
    11581 gggcggcgcg tacggggagg gcgaccgcga gacgttcacc tttgacggag gggatcccgc
    11641 ctacagtgac aaggccgtgg cgtgtgacgg aaggcatgtg acgctgaagc ctggttcctg
    11701 gcgtcacggc gaagcgcatt ggatcggcgt accgctctac atcatcggtg gacgcggtgc
    11761 gggacagatg cgctgggtca ccggtatccg ggaacgtacg gtggagctgg accggccgtg
    11821 ggatatccag cccgatgacc agtcccattt cgtgttggcc cagatgcgta gtcagttgct
    11881 ctttctcgac aataccgacc gtgatggtaa tccgttcgcc ctgtacggga ccgccagcga
    11941 cgtggtcatc gcaggtaaca gcttggagcg gaccggcggt ctgcacgcgc acggcatgtt
    12001 caaatctgtg cccgagcctt cctggtttgt ccagtttttc gggaaccgca ttgtggaggg
    12061 gaattcggtg cgcggcccct tctcgtacgt ggtcccggcg agtgattcct ggctcggctt
    12121 tttcgaccgg ggtatccgca ggcctttgac ctatccccag aaccgggtcg gtgttatgcg
    12181 ggggaacacg ctggagaaca atgcgtttct ggctgcgcac gggcgcgtga aaaacatgct
    12241 gatggaaaac aatctggttc gggaagcgga ctgcggcatc gtgatcgagt cgcgtgtgca
    12301 ggacgcgatc gtgcggggta accgtttcga aaacgtcttg cggccgctgg tcttgcacga
    12361 tcaggtttta tgccgtgcct cggaacggct ggtcgccgga ctctccgctg tggacgcgct
    12421 ggcgtccgac gtggtgccgt cggcggcgtg gcgggaactt gccgatgcag cggtttcgct
    12481 ctcgcgccgc aggctgccgg atgcggaaga ggtcacggag acgcggactc tgctcgcgcg
    12541 cgccgcgtca cttcttgctg cgcgcgttgg cgaccggaag ctctccgcgg ccgtcgtcga
    12601 cgcattgttc ggtttggatc tgacgcagaa aagcgacgct ctcttttcgc gtctccagag
    12661 tggcaaggag gtgaaggttc ctttgggcgg cacatatccc gtctgggcgg tgccggccac
    12721 gctctctgcg gcggtcgagg gttttgaggg gtggggggta cgcgtggcgt cgcccgtgaa
    12781 actcgtgcct gggggacagt gcggcttcgc cctgcatgtc acccgtccag atgaccggcc
    12841 gcaaacattt gcgttgcccg tccggttcac gctcgatggg gaggggtgga gcgtaacgtt
    12901 cacggaaacc taccggcagg acacgctgaa tgtgacggat ttcctagtcg ccggtccctt
    12961 ccggaacgcc agcggcaagc cgattgacag tgcggttcat ccgccggaga gccggctgga
    13021 tgtgaccgag tcctacgaca cgctggacgg ccgcaagccg tggcgggccg tgcgtgccgc
    13081 caagccggac ggaacggttg acctgaacac atgtttccgg aacacggaac tggccacggc
    13141 gcatgcggtg gccatcgtat acgcggcgcg tcctctgcgg gttcgaatgc agtacggctc
    13201 caaccagaac acgctggcgc gggtcaatgg cgaagccgtg ggctccagtg cgcggcgtga
    13261 tgcgtacaat ggacgccccg tggacttgcg ggagggggag aatgtgatcc acctgatcag
    13321 cacgcatgcc acgggtgcct ggcccctgaa agtccggctg acggcggttg acgcgactcg
    13381 tcccggcgaa ctgcgggtgg tttcgtccga ccgacttttc cagtctgacc ggctcaaggc
    13441 cgaagggcgg cgcggggttg tgggcgagcc cttcacagat ggggagggac gcgtctggcg
    13501 gttgtcgcat gaggacacct tcaaccggct gcgtgtgggg ttgcagtatg aggggcggga
    13561 cccgatcgaa tggcgtgacc attcggtacg cattctcgac ggtcgattgg tgggtgcggg
    13621 acggatcaca ctgaatctgc gcctctcggc tcctctccgc gtggagtatg atgttccggt
    13681 cgtgaatggc cgcgcgaaca ccgggctgac attggccgcg cgcggagcat cagctgtggt
    13741 gcgggattgg ggagaggcca tgaagcaaaa acaggtcgtc acgttgaaca acgtgctggg
    13801 tggcctgacc ggggagaccg cgcgggtggt ggccgtgttt gacacaaaag ggagcacgct
    13861 ctttatcagc ggaaaaaaaa tgcgggaggt tcccgacacg gagtgggttc gcgggttgga
    13921 tgaactcacc ctgagcggaa cgctggacaa tctgcgcatc cataccgggg agaggggcaa
    13981 gccctgaaag acacggttcc gccggtcggt ctgtacgatg cctgattctg ctcccgacag
    14041 tagcgtttac cgctcccgac agtagcgttt accgctcccg acagtagcat ttaccgtccc
    14101 cgacagtagc atttaccgct cccgacagta gcatttaccg tccccgacag tagcgtttac
    14161 cactctggca tcttgcccac ttctcatgag aagtgggcag atccgcagat ccggtaccgt
    14221 cacgcggccg cttgaccccg gcactcgttt tttgataaaa aaggacatcc atagtggttc
    14281 gcacaagcgt acaccctgaa caaggagtaa aaagatgtca atccaggctt cccgtcgtaa
    14341 ttttttgaaa ctctccacgt tggccggttc cggcgtgatg gccgcgaaag ccgtggcacc
    14401 gctgacggca caggcgcagt cggccgctgc gggccagacg gtcatggggt tccgggctcc
    14461 gaaactggag cgtgtgcgcg tgggctttat cggtctgggg cgcggtagtg gccatcttgg
    14521 cgatatgctc aagatcgaag gggcagaggt cacggcgctg tgtgacatcc accagcccac
    14581 gctggatcgc gcgctcaagc gcgttgccga tgcagggagg ccgaagccgg cgacgttcag
    14641 cgacggtccg gaggcctaca aaaagatgct cgaacgtgac gatgtcgata tcgtctatat
    14701 cgcgaccccg tgggaactgc acacgccgca ggcgcttgat acgatgcggg ctggaaaaca
    14761 cgctttcctt gaggttcctg ccgcgatcac gacggaagag tgctgggcgc tggttgatac
    14821 ggcggagaag acgcggcgtc actgcatgat gcttgagaac gtcaactatg ggcgcgaaga
    14881 gttgatggtg ctgaacatgg tcagacaggg gcttttcggt gagttgctgc acggtgaagc
    14941 cgcctatatc cacgaactgc gcgggcagat gaatcaggtg gagcacggca cgggctcgtg
    15001 gcgcaccttg cactatgcgc ggcgtaacgg caacctgtac ccgacccacg gcttgggtcc
    15061 gatagcgcag tatatgggca tcaatcaggg agaccgcttt gattatctca gctccgtttc
    15121 ctcgccggcg ctcggccgtc cgctttacgc caaagaacgt ttcccggctg atcacaagtg
    15181 gaatcagatc aaggggtggc gttgcggcga tatcaacacc acgatcatca aaaccgtcaa
    15241 gggccgctcg atcatggtgc agtgggatga gacctcgccg cgtccctaca gccgccataa
    15301 cctgattcag ggtacgcgcg gcaccttcgc ggggttcccg aatcgtatcg cgctggacta
    15361 ccgcaaggag gatttgcccg aaccggtgcg caaggcgctg ggcgataagc cccgcacgaa
    15421 ttatcacggg tgggatcaaa acatggcacc gtggttcgaa gcctatgacc atccgctctg
    15481 gaagcgtatg ggtgccgagg cgaaacgggt tggtggacac ggcggcatgg acttcatcat
    15541 gaactggcgc gtgatttact gcctgcgcaa cggtgagccg ctggaccaga cggtctacga
    15601 cgccgcggcg tggagcgcga tcggtccgtt gagcgaacgc tccgtgactg agcgtagccg
    15661 gtcggtggat atcccggact tcacccgcgg ggtgtgggaa cgcacacagc ccttcggcat
    15721 cgtgtcgtaa acgacgaacc gaccctattt cggaatcagc atggggtcgg cgagcaccac
    15781 gtagttggcc ttggagcggt tgatcggaga ggcgagaaag gccaggttcg tgacaccggt
    15841 gatcggaagg tcgatcggta cgctctgcgc cgtgccgcgc aactccgtct tgtgcagttc
    15901 ggcatcgttg ccgaaaaccc ggaaggtgac ctggccgtcg gtgccgaggt cggcgtgcaa
    15961 acccacgcgg agcatgattc gcgaaaacag gttgccgggc aggaaccaac tgatacgttt
    16021 gacgccgatc cccaacccct tcacttcggt gcgttcaccg ctgatttgga gcgcgagggg
    16081 tacgggatct tttccgtccc ggaggatcat gtcgcgttgc ggatggcccc agtacggcga
    16141 gctgaaaaac gcggcctgcg ggtaaaaaag cgacggggcc tcgttcggcc agtacggggt
    16201 cagcgcgatc ggcgcgggcg ggggcgcctc cccgcgggca agcgcgcaga ccgagcggaa
    16261 ggcgtcggcg acgaggtcag cccccagaat agcggagggg cgctgggccg cggcggaggc
    16321 ctgcttgtct tctgcataaa ccgctcggac gatgtcgagc acgcgtccgc gcgcgaagac
    16381 cgtgccagcc tgagcgcgtg acagaaggcg gaacgccacc tcctctgtgg tggcacccag
    16441 cgcgacaggc gtgtgcgtgt agcccgacag atcaaagccg ccgctttcaa ggtgcgcgtg
    16501 gagaagcatg tgctctttcc cggctggggc gggcatgtac tggccgagaa gccggtacat
    16561 gttgtcgccc acggatgagt gcgcgggaca agtccaatct tccagcatat gcgcgagcac
    16621 accgaggtga cgagccgcag aggccgcatc gtttgtttta aaggacgcga ccgcatggcg
    16681 gatgtagtat tcgagcattt catagttgga tgcggcattc cacgggaggt ggaagttctc
    16741 aaagcgtccg gaataggact ccgttcccat gaaatcccaa acaaagcgct cgttgccgtc
    16801 ctttttgtag acccaatccg gaatcaggca atatccggca ctgagcggct cacgctcatt
    16861 gccaagcagg gccgcgtcgc tggacggaag cgtcgacagt gcggcgcggg tgatgacgct
    16921 gtgcgcgtcg ccccatccga aagcggatac ggcggtcagg atcgcgaaca gaagaaagtt
    16981 cagcgcgaat tttctgtgcg tgtctttacg cattccttat gtcctcgatg agatgggaat
    17041 cggggggggg ggcacgttgc ggcacgatca ggagggcgga tcggactgca atgttttgaa
    17101 gacaaggaaa agacagaaac cggtgatgac cgtccagcag agtaccatcg taatccagcc
    17161 agcgagggtc atgcgtcacc ccccttccat gcagttggca gtgcgtcgag caccatctgc
    17221 accacttgtt cgagcgtcaa gtaagtgctg tcgatttcca acgcgccatc cgctttgcgc
    17281 agcggggcgt gttggcgcgt ggagtcgatc gcgtcgcgcg ccaacagcga gcgcttgacc
    17341 tcctccgccg actggttggc gatgcctttc tcgacctcct ccttgcggcg gcgttcggcg
    17401 cgcgcctcgg cgctggccac gagatagaag cgcgccggcg agtccggaaa cacggcggtc
    17461 gtgatgtcgc gcccctccac gatcaggtcg ccgaagcgcg tcatgccgcg cagccagccg
    17521 gtcacgcggt cgcgcacagc cttgaccgtg gcgactgggc tggcgtggcg gttgatctcc
    17581 ggtgtgcgga tggcatcgcc cggctcttgg ccctgcacgc ggtagatgat cgcgccgtcg
    17641 cgcggttcgc actcgacttc gaccttctgc gcgaaggcgg caaccgcttc tggatcggac
    17701 gtgtcaatgc cgttctgcag gcactgccag gtcatgatgc ggtagagcgc gccggaatcg
    17761 acatgcaaaa aaccaaggtg ttggccgacg cgcctggaca cagacgactt gcctgcgccg
    17821 gaggggccgt caatcgcgat gactcgactc atatcggggt tcctttattg atggcctgat
    17881 cttaccactc agaacggtgc ggttcaagcg catggtacgc accatgcgga tttaagattt
    17941 aggatttagg atttaggatt taggagttag gagttttttc acttcggcgt tgaacgttgg
    18001 gtgttgagcg gtgaaagttt tttgggcagt gccgcaagtc gcaagtcgcg ggtcgcgagt
    18061 caacctctct tattgcgacc acgccacagc cttcccgata tccgcctcgg ttatgttgag
    18121 ctgctccatt ttttcatgca cggcgctgag aggccgaagg gtcaattgat ccttactggt
    18181 gagtttggcg agcgtcacaa caactccttt tttccttaca tccttataaa aaggacgcgg
    18241 gggcgtcaag gcgaacgaac gcccgacgcc gaagcgaaac cagaaaccgg aggagacggc
    18301 ctccgggtta ccgaatgttg gcgtggtacg cctgaatgga tttcacgccg cccgcgctgt
    18361 cacgcgccga acggatgccc tgcgccgcgg ccgccgcagc cgcgagggtg gtgaggtagg
    18421 gaaccttgta tttgatggct gccttacgga ttgaggcatc atcggcacgc gcgtcacgac
    18481 gcgcggaagg ggtgttgatc acgagacaga cctggcggtt gacgatggca tccaaaacgt
    18541 tgggacggcc ttcgttgatt ttttggacca cttcgccggg tacgccgtgt tccttgagcc
    18601 agaggatggt gccctcggtg ccgatgatgc ggaagccgag tttgacgaaa gcgctgatga
    18661 ctttcgccgc ggagtcgggc ttttcggaga ggctcacgag cagtgtgccc tcttccgtcg
    18721 gcagccgcgc gttggcggct tcctgcgatt tgtaataggc gaggccgaaa gtcgaagcca
    18781 tgccgaggac ttcaccggtc gaacgcatct cggggccgag gaccgggtca acctccggaa
    18841 atttgtcaaa ggggaaaacc gcctccttgg caccgtagtg cgggatgacc ttgggtcgta
    18901 aatccagatc ggcgatggag gcgcccaaca tcaagcgtgt agcgatgcgg gccatctggg
    18961 tgtcacagac cttggagacc agcggcacgg tgcgcgaggc gcgcgggttg gcctcgatga
    19021 catacaccgt gtcgttctcg atggcgtact gcatgttcat gagtcccacc acctgcagct
    19081 cctgggcgat tttgcgggtg tacgcgagga tggtgtcgag atggcggtcc ggaatcgaga
    19141 ccggcggcaa cacgcaggcc gagtctcccg agtgaacgcc tgcgagttcg atatgttcca
    19201 tgacagcggg aatataaacc tcccgaccgt cggagagggc gtcggcctcg cattcgagcg
    19261 cctctttcag gaagcggtcg agcaacaggg gccggtccgg ggtgacgccc accgccttgg
    19321 ccacgtactc gcgcaacatc tcctcgtcat agatcacctc catcgcgcgt ccgccgagca
    19381 cgaacgaggg acggatcatg atgggatagc cgatggaccc ggcgacccgg atggcctcat
    19441 cgatattggc ggccatgccg gattcaggca tggggatcgc gagccgctcc atcattttgc
    19501 ggaagaggtc gcggtcttca gcggtgtcga tggagtcgat gctggtgccg aggatcttca
    19561 caccggcatc cgcgagttcg cgggcgatgt tgagcggggt ctggccaccg aactgcacga
    19621 tcacgccttc cggtttttcg ttctcgtaga tttggagcac atcctcgagc gtgacgggct
    19681 cgaagtacag tttgtccgag gtgtcgtagt ccgtggaaac cgactcgggg ttgcagttga
    19741 ccatgatggt ctcgtaaccg gcatcgcgca tggcgaaggc cgcgtgcacg cagcaatagt
    19801 cgaactcgat accctgt
//
LOCUS       DAOSLE010000039        19770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_2997_length_19770_cov_7.288461, whole genome shotgun sequence.
ACCESSION   DAOSLE010000039 DAOSLE010000000
VERSION     DAOSLE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 19770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19770
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2997_length_19770_cov_7.288461"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1252
                     /locus_tag="PK576_04310"
     CDS             401..1252
                     /locus_tag="PK576_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE60846.1"
                     /translation="MRYSRREFLQAGATGLAWAVAPRSAQAAARVAKQRKIQVGVQLY
                     SVRELCKKDLPGTLKAIKEMGYAGVEFAGYYGKNATEIKQLLDDNGLVMCGTHTGVDT
                     IRPQNLAKTIEFNLVTGNRYLMVPGMNAKDAQGWIDFAKLFSDAAVTAKAAGMYVGYH
                     NHQHEFKDKFDGKCKWEIFYTHASPLVCQQMDVGHVVSAGEDPVAWLKRFPNRSRTLH
                     AKEIYPGPGILGQVPEGKQGVKWDEVFAAAEADVTEWYIVESEADPNTLEKIRGCIDF
                     LKSKGRA"
     gene            complement(1491..3689)
                     /locus_tag="PK576_04315"
     CDS             complement(1491..3689)
                     /locus_tag="PK576_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60847.1"
                     /translation="MKRELIAGIAVAAVAAGAVELHVNPSAAPDGDGSAARPFATLVG
                     ARDGLRAARKAGTVTKDARVDIVLAPGDYVLAAGLALDAQDGGASADAPVTWKAARPG
                     TARIVGACRVPVAACRPVTDPAVLARLPEEAHGKVYEADVAALLPAELPPMAERFGAT
                     PTAPLLFINHKIATLARWPNDDFTSFSKSVDRGAVLKRHAGNSAEYGPGAFIYENPRA
                     KRWNFAQGVWMNGYWTHDWDNYSVRAAAYGVENGTNDVVRLATGVPYGVMGGTWGRKG
                     RRFYVFNLLDELDVPNEWYLDRVAKKLYFYPPNGTLAATDEVFLASLNEPLVKTAGSI
                     NHLRLQGLVFEYTYNTGLSLAGQGIQVLDCRVMCCGASGMSVHGNRNVIRGCEIAQVA
                     RVGLSVGGGDRRTLQRAETVVEDNHIHDFSMMQRTYAAGVSVSGCGITLRANVIHDAP
                     HMAVIYGGNEHVFEYNNVYRVLLETGDAGAYYTGRDWTTQGNVLRFNYTHDLGAEGEL
                     ANTMGFYFDDCDCGDAVYGNIFHNVARGILVGGGRDHPIRNNIFSRCLVGLSIDCRGM
                     TWKHWNSMEHGGPSWMLEDKAKALSYTNGVWAERYPRLADIMNDYPREPLYNPVEENI
                     FIDCTKQLLALDGKAPLERMASIARNVVVNTRGTNGVAWAAPDKRIASAFIILNGTTN
                     APCSFGFADAANGDFRFLPGAEILKVCPGFQVLPLDRIPADAGSKTTRDR"
     gene            complement(3716..6649)
                     /locus_tag="PK576_04320"
     CDS             complement(3716..6649)
                     /locus_tag="PK576_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOE60848.1"
                     /translation="MKKACPRTTVGRRFVVALAAALGIVGAAFAVPTAPTLKMTGFFN
                     GKLTFGVKYSPVTDHTLEVQMKDQGAADDDYVTIYTKAVNSLAKFLANKYDAAYFTYA
                     TNFAGTATFRMRTTLNGEASDWVAAGDFTAYVNVTGATNSNATTFGNAVDGDMYTNVD
                     EPSNPWVGYDYNAAIRISRIRVISRPDLISRFYGAKVQIASDATFADATTVFTSSSAN
                     TTPGVPIEIVFDEPVTAKSVRVLSGTASGCRSSVCEFEVVPADVPFKPVLTVVGYDDV
                     TNFYPVLTWTVPAELHATKATLEYATGSAGPFTALATDVPIAITTFTNTAAKVGVLGY
                     YRVTVDCNHPLFPGQSATSDAVTMGRCRRLDRSWGNETTLLDGVTVMPATNGVYLSDH
                     YPKAFDGNPDTFPDIFSGGTYSRGPVGLDFGEPAWVTSFGYICRNDNWCYVRIKNTAL
                     FSADDGDVELTNKVQRSEKVTQASQDKTFYTQMGTSLPDNGARKWFLYAPESGPSEFG
                     GNVAELMFFGWTQSDLVAAGVVTAPTTLGFARSADGLSVDVGWNAGINVVQYRLERRV
                     YGTNVWGTVATVAGTSLACNDNTVSQNIYEYRVVSIGSNAVEAVSEPFTYTYYAPGTG
                     TGLRGAVYAPYVANVAAMCQPEKYYELPTGPVNLSVAKGVEIVAGSSITNNMRLLWRG
                     KLVTPFTGAYTFTLVTTDGGALYIDKKSVANAWTDGPKTTSGMVELTAGEHDIAVDAR
                     LQVSPVSCVLKWGGVVPDEVIPATQLIPAAEAATLTVDGWTQRTYNGQQYGNAYVTAN
                     NAYMISSSKEAPSDRNKLNATFLAKTARYSFDISARFRAILNGEAGIMARVPNGNFYA
                     VEFKYDGSGFSYYGVRGVTNNSDTINMLLPWKLTDTNDFSNVDYDLRLVYDSAVRTFH
                     AYAKDNNKTEWNLIHTWHNDRQITGDYEYGFVVHGASGNPVSSFTVTPLSIRLHRPGL
                     TVLFK"
     gene            complement(6908..9550)
                     /locus_tag="PK576_04325"
     CDS             complement(6908..9550)
                     /locus_tag="PK576_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE60849.1"
                     /translation="MLFGRSGFIFASLCVGFRVWAGEPVTVTFLQGFDPDRPENPGTR
                     QILRFAQREPRLNPQKWGGLELPGAGGRAPFMLSLAGGTAPDIYFCWFHIIRHDIEQG
                     FLHPLNEWLGEDLDGDGQLGDHEARWPGWKRVPPLWRQAATRDGKVYGLPMAGIWYYG
                     IVYRKDLVQQAGVDPESVPATWDEFFRWCQRLTFPGKRIAGARVQRGQRAFGAENRPW
                     GWLPWVQAAGGSPIVNLRVSPATGRTYRFPMEETRFIAPDTGEDLSGVPGVWQAAFDS
                     PEAIEAAEFFHRLAWAPWLRDPQSGEPVDLSPEALAAGCVTLPDGREHRFQPGEVIRG
                     VVRALPRLTQDLPQLFAQGEVVALFSGAELVEKLTRDLNLPPDMVGIMPFPAARPGLK
                     PVFQAHKHFYSMTEGVGRRPKAERDMVWACVEQLASEEVNDEAVRQKVLEGHARWCTP
                     TELTRLGFDEYLAEVPAGIRRNYERLEAGEILARTEPFAGFWQAVSDLLDRRFLGLLL
                     ADTGERFDVAAALREINAEANRGLMFRASETMLARRRPLARVLFGVGIITLLGCAALI
                     VRRDARDADASAVSARRIGAMRVRHGLSPWLMLAPAVLSVALWSYYPLLRGAVMAFQE
                     YKIVGETRWAGLDNFILVATDAGFWDAWGRTLRYVGLTLLLGFLTPVLLALMLAEIPR
                     GKVFFRTVYFLPHLTSAMVIALLWKLMYDPTENGMLNRILLGLGASRQTWLQDPGLAM
                     VCCILPGVWAGAGMASLIYVAALHSLPPDYYEAAALDGAGILSRFRHVTLPQILPLMV
                     INFVGAFIAAFQGMGSIFLLTFGGPGDATNVLSLAIWKEAYNNLRFSTATTMAWFLGV
                     ALIGFTYLQIRILRKVEFRRAAAN"
     gene            9935..11149
                     /gene="trpB"
                     /locus_tag="PK576_04330"
     CDS             9935..11149
                     /gene="trpB"
                     /locus_tag="PK576_04330"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE60850.1"
                     /translation="MNVPRPLSGNPDAKGFFGKYGGQFLPPQLEKPFAEITEAYHRIA
                     NDAAFISELRLIRKHFQGRPTPVYHARKLSLQNGGAHLYLKREDLNHTGAHKINHCMG
                     EGLLARFMGKKKLIAETGAGQHGVALATAAAYFGLQCEIHMGEVDIAKQAPNVSRMKL
                     LGAKVVPVTHGLKTLKEAVDSAFEAYLADYENSIYCIGSVVGPHPFPLMVRDFQKVVG
                     IEAREQFIGMTGNLPDAVCACVGGGSNAMGIFHGFLDDPCEIYGVEPLGRGPKTGDHA
                     ATMSYGREGIIHGFKCYLLQNEDGSAAPVYSCASGLDYPGVGPEHCHLKDTGRVRYVT
                     CSDEACRDAFFKLCRLEGIIPALESAHAVAFALTQAREMESGAILVNLSGRGDKDLDY
                     VIENWGVGETEA"
     gene            complement(11313..12764)
                     /locus_tag="PK576_04335"
     CDS             complement(11313..12764)
                     /locus_tag="PK576_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOE60851.1"
                     /translation="MNKRAVFHLVSYMTLFIGLAVTACAAVSHVCGDALEVRCAFLLA
                     GGITLAVSLVAGMLTRGDLNLGRRDGFGVVTLGWLSAALFGALPYLLTGVITHPVSAL
                     FETVSGFTTTGASVLDNLENLPRGILFWRALTQWLGGMGVLVLCVAILPFLGVGGMQL
                     YRAEMPGPFKDRLTPHIATTAKLLWAVYLLLTVVQTLLLKGVGGMGWFDSVCHTFATV
                     ASGGFSTRTASVAAYDSATVEGIITLFMFLAGINFSLHLSALTGRPGHYVRDPECRAY
                     FLFLTGCCLFMTANIWATGHGSFARCLRDAFFTGTSVLTSTGFCTVDFDRWPGASRLL
                     LVFMMLMGACAGSTGGGIKVVRIMIVFKKILREIRLFMRPTAAIQVKLGPKPVEPDLV
                     LHVSAFVIIYFAVFALGSFLMTFFTPDLETATTSVITALSNVGPGLSAVGPVRNYASI
                     PLAGQGVLATCMLLGRLELYTVLVLLFPSCWRR"
     gene            complement(12775..14124)
                     /gene="trkA"
                     /locus_tag="PK576_04340"
     CDS             complement(12775..14124)
                     /gene="trkA"
                     /locus_tag="PK576_04340"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOE60852.1"
                     /translation="MNSVIIGAGHAGRLLASHLCRERHSVVMVDESAEALEQAEASLD
                     VLTVCGSGADPAILEKAGAGRADLFVAVTDRDEINIIACLLAHSEGVPRKIARVGSAQ
                     YDPGAVKYNLTKLGIDLLINQKFACAKEICNALLLPGAVESFDLFGGRVLVAGFGIVG
                     GSPILGHTPATLPDQDLVRKLRIIAIRRQGRLVIPRGDTAFLPEDVLYLVGAPDDIRV
                     FSTWMDPVREAFEKVIVAGGGDIGLAIAKNLERIGLHVVLLEQDEERALFCSGELDHT
                     LVLRADALSGSALDETGIVPGTAFVAVTGDDEDNAMNCLMARKKGADFVVTQIARTDY
                     IPIMENLGLVDRIISPYESITNAILHYLRSRDVRAAALLHDLPGELLEIELSPTSRYA
                     GKRVSDIRFPSGSIVAVVQRGDGVLPATGGLCFEAGDRLLIFADPAAVKKIQAMFRK"
     gene            14429..15607
                     /locus_tag="PK576_04345"
     CDS             14429..15607
                     /locus_tag="PK576_04345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOE60853.1"
                     /translation="MNKTSGTGWRAVLCALCTVWGAGVADAQHVTGQTKPVLPVVDLS
                     GETARHVVIAAGTPEEYQGHPTTVLLPDGKTVFCVWCINHGGAAGPMARSADGGKTWV
                     RLDTTLPPGFRTHQNCPSIYRLIDPQGKARLWVWSAAKDTRQGTPMPSIMSEDDGEMW
                     REMPPLGEAFRCVMTFSSMVRLKDGSYLGLYHRGEGKGEGRKFLEVVQSVTRDGGFTW
                     SVPRVVANVAGKMACEPFVFRSPDGSELCCLMRENTHKGHSLQMFSRDEGATWSVPED
                     TPWGLTGDRHMGVYAPDGRLVIAFRDQAPESPTKGHFVAWVGNYGDIRQRRDGQYRIK
                     LLHGYKRVDLGYPGVERLADGTLLATTYLKYWEDTRQHSVVCTRFTLAETDAKAGLNP
                     "
     gene            complement(15651..19652)
                     /locus_tag="PK576_04350"
     CDS             complement(15651..19652)
                     /locus_tag="PK576_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60854.1"
                     /translation="MRYAIPNLIHVLCVIGFFAGGLIRAEDGTVQQTGNFSTPATWVD
                     GVIPGGAGATLYVKGNYTLNNFFTNAVLGHIVTPADTPVAGLTSVFTVSLPWENRTSG
                     NPDPVYGTYRFGVFTNYLTLDSGVEGEPATIRCLPLLTSNFRYAINIPLLLKSDLEVQ
                     HNGRASGNWNNPLTAVWLGRDIREEGGRRGIRVRFDKEDMQLMLKGNNTFSGDLIVEK
                     GIVRAGDPFRYNVPGNQFGVDNTLIATSGVATVDVNGFHFGPEKPILIGGRGANGMGA
                     LTSGCQSPGVTSVWSGPVTLVSDAAVAGTVSRYPRSEGGNLRIDGAVTDGGRGCRLEN
                     VSLRNLVLAGNNTYSGGTLLTSGFLTALTPSSLGSGPLIFNGGAFRIDSSTGVDPSAF
                     TLVSTNSKPVQLLTDGADFTLGHALTGFTSGLTKSGLGALILKQDNAYTGTTAVVLGD
                     LILDYSEAGGQKTPADGTITLGNNARLILRNGQTATPDQAVRTLSTYKGASARVIHDS
                     AMPLAFNFLTPNTDSVLNLEGEGPYKLIQTSNGSENSAYPNILPANILFKGRDWAVRT
                     ASGNAISNFTDYASDWSGSNKHVDVTAGLAQSVPANLEVATLRFNTPNNGNPIILNLS
                     GTNQIKSGAILVTEAMGNTPVHITGGAIARMGSGNLIIYNFNTQAVVRIDSQLLGFPV
                     FNTNDLGQVTGITLSDVGVMKAGPGKVILGNADNRHDDYTSVLEGELEISGPECLGVG
                     SWHGSWKSVQNFVMIYNGGTLTTTGSFDFAREVYPGYTNITTLDINSAGGVLNVSGAG
                     DRVANLDANPTPVLRNGGRLVKRGAGTWFVGRGFGSGADRAEDTVTAREYTMFFDVEE
                     GSIEHRAGTTSANSFLGEGSMSMTVRNNAVLKGGGFLYGNNGGGNGGGNPEDVAIRKV
                     LHIDAGGAIVDLNGITVNMGWSLFDGLTRTSDWLEGPGTLTITNSSATGASFRFYNLL
                     NHAFTGRFDASGLMAADATYGGGLPNGELVVPEHRVYSFTSCYPSHYPLHFGRVTGSG
                     AFGGRGISNSDSDNVALIGCDDNGVFEFEGYLWGTWGSSNRGAFRFVKAGNNTWRISG
                     TTNAMTTSVTLRKGTILVGADSPGNGTVGALGTERVILSDAGTAPGDALSLLTDGPYT
                     VGNGIILMDYAPGALMTLGGNQTTGASAFTGDLALTRDVCLHAANTDDSGVTFSGAIT
                     GPGGIIKTGPGKVILTGSVQNTAPHTVLDGTLMIMGDTTLTNALVVAAGASGSGTLAV
                     TGNLTIGEGVTLTLQPDSLVRGQTYTLATWTGTLSGSFASVTGLPDGWHVGRFPDHLE
                     LYYAPPGTQIRIR"
BASE COUNT         4018 a         6218 c         5976 g         3558 t
ORIGIN      
        1 aaatcctaca tccgcgcgta gcgcggaaga cgcccgcgct cccgactgcg cacacgcaaa
       61 ccacgcacta aactcgcata ccccataccc ctgtcacgaa caaccaaggg ttgcgaagcg
      121 ttttcgcggt gtggtaatgt gttgccgtgg cgaggcgacc gaggcagcaa cggaaggata
      181 gcggcgcggt tgaaaaccga caaagccttg tatcggtcag ccgcgcgcgt gttgaggttg
      241 ctggaaacac gaaatgtctc atgtcggttg cggggagatg ctatccgaaa acccggcctc
      301 ccctgtttta cgattgtgcg aaaacccgct tttctgccat actctctcca tacgccttcc
      361 cggagtggga cgaggcgtct gacagaaagg agagtttaca atgcgttatt caagacggga
      421 gtttctgcag gctggtgcaa ccggcttggc ttgggcagtc gcgcctcggt cggcgcaggc
      481 ggcagcaagg gtggcgaaac agcgcaagat tcaagtcggg gttcaactct actcggtacg
      541 ggagctgtgc aaaaaggatc tgcccggaac cctcaaagcg atcaaggaga tggggtatgc
      601 gggcgtggag ttcgccggat actatggcaa gaacgccacg gaaatcaagc agttgctgga
      661 tgacaacggg ctggtgatgt gcggcaccca cacgggcgtg gacaccatcc ggccgcagaa
      721 cctcgccaag acaatcgagt tcaacctcgt caccggcaac cgatatctga tggtgcccgg
      781 catgaatgcc aaggacgctc aagggtggat cgatttcgcg aagctcttct cggatgccgc
      841 agtcaccgcc aaagctgcgg gcatgtatgt gggctaccat aaccaccagc acgagttcaa
      901 agacaaattc gacggcaagt gcaagtggga gatcttctac acgcatgcgt cgccgttggt
      961 ctgccagcag atggacgtcg gccatgtggt gtcggccggc gaggatccgg tggcgtggct
     1021 gaaacgcttc ccgaaccgct cccgcacatt gcacgccaag gagatctatc cggggcccgg
     1081 catcctcggc caagttccgg aaggcaagca gggcgtgaaa tgggatgagg tgttcgcggc
     1141 cgctgaagcg gatgtcaccg aatggtacat cgtggagagc gaggcggacc ccaacacgct
     1201 cgaaaaaatc cgcggctgca tcgacttcct gaagtccaaa ggccgcgcat aacccgttcc
     1261 ggactggaac gcgtgaaagc ccctccccga ccggctccgc cggatcatcg ggaggggttt
     1321 ttcgttatcc tgatcctgca caacgcggtt gatccaacag gccgtccgca ccgtcggtgt
     1381 cgccgttggc ccatatcaaa accatcttct tttgcgcgat gtcccagacg taaatcgcgg
     1441 ttctgctctg cggcacggca gcgacattgg acgatgccgc ctatgggatg ctatcgatca
     1501 cgagtcgttt ttgatcctgc atcagcaggg atgcgatcca gagggaggac ttggaaaccg
     1561 ggacacactt ttaggatttc ggccccggga agaaagcgga agtcaccgtt cgccgcatcg
     1621 gcaaaaccga aagagcaggg agcgttcgtc gtgccgttga ggatgatgaa ggcggaggca
     1681 atgcgcttgt ccggcgcggc ccacgcgaca ccgttcgtgc ctcgcgtgtt caccaccaca
     1741 ttacgcgcga tggacgccat gcgctccaat ggagccttgc cgtcgagtgc aagcagttgc
     1801 ttcgtgcagt cgatgaagat gttctcttcc accggattgt agagaggttc ccgggggtag
     1861 tcgttcatga tgtccgccag acgcggatac cgctcagccc acacgccgtt cgtgtaggag
     1921 agcgccttgg ccttgtcctc caacatccac gaggggccgc cgtgctccat ggagttccag
     1981 tgcttccacg tcatgccgcg gcagtcaatg gaaaggccca cgaggcaacg cgagaagatg
     2041 ttgttacgaa tgggatgatc ccgcccgccc cccaccagga tgccgcgcgc gacattgtgg
     2101 aagatgttgc cgtacacggc gtcgccgcag tcgcagtcgt caaagtaaaa gcccatggtg
     2161 ttggccagtt caccttccgc gccgaggtcg tgcgtgtagt tgaagcgcaa cacattgccc
     2221 tgcgtggtcc aatcgcggcc cgtgtagtac gcgccggcat cccccgtctc cagcaacacg
     2281 cggtagacgt tgttgtattc gaaaacatgc tcgttaccgc cgtatatgac ggccatgtgg
     2341 ggcgcatcgt gaatcacgtt tgcccggagc gtaatgccgc aaccgctcac gctgacacca
     2401 gccgcatagg tacgctgcat catgctgaaa tcatggatgt ggttatcttc cacaacggtc
     2461 tccgcacgct gaagcgtccg acggtcgccg ccgcccacac ttaagccgac gcgggcaacc
     2521 tgcgcgattt cacagccgcg gatcacgttg cggttgccat gcaccgacat acccgacgcg
     2581 ccgcagcaca tcacacggca gtcgagcacc tgaatgccct gacccgccag actgagcccc
     2641 gtattgtacg tgtattcaaa aacgagcccc tgcaagcgca ggtggttgat gctccccgcc
     2701 gtcttcacaa gcggttcgtt gagcgaggcc agaaacacct cgtcggtcgc ggccagcgta
     2761 ccgttgggcg gatagaagta gagctttttt gcgacgcggt cgaggtacca ctcgttggga
     2821 acgtcaagtt catccagcag gttgaacaca tagaagcgac gccctttccg cccccatgtg
     2881 ccgcccatca cgccatacgg cacccctgtg gcaagacgca ccacgtcgtt cgtaccattt
     2941 tccacgccgt acgccgcagc acggacggag tagttgtccc aatcgtgtgt ccaatatccg
     3001 ttcatccaca cgccttgagc gaagttccaa cgcttcgcgc gcgggttttc gtagatgaac
     3061 gcacccggcc catattcggc gctattgccg gcatgccgct tgagcacagc ccccctatcc
     3121 acactcttag agaacgacgt gaaatcgtca ttgggccagc gcgcgagcgt ggcgattttg
     3181 tggttgatga aaagaagcgg tgccgtcggc gtcgcgccaa aacgttccgc catcggcggc
     3241 agctctgcgg gcaacagagc cgccacatcg gcctcgtaca ccttgccgtg cgcctcttcg
     3301 ggaagacgcg ccaatacggc gggatcggtc acaggccgac acgcggcaac cggcacgcgg
     3361 caggcaccga cgatgcgggc cgtaccggga cgcgcagcct tccaggtaac aggcgcatcc
     3421 gccgaagcgc cgccgtcctg cgcatccagc gcaagccccg ccgcgagcac ataatcaccg
     3481 ggtgcgagca cgatatccac acgcgcatcc ttcgtgacgg tgccagcctt ccgcgcagcg
     3541 cgcagaccgt cccgcgcgcc gaccagtgtg gcaaaaggcc gtgcggcaga cccgtcgccg
     3601 tccggcgcgg ctgaagggtt gacatgaagt tccacagcgc cagcggcgac tgccgcaacc
     3661 gcaatacctg cgatgagttc tcttttcatt gattcccttg ttttcaaacg agcttctact
     3721 tgaagaggac ggttagcccc ggccgatgca acctgatcga gagcggcgtc acggtaaacg
     3781 atgagactgg attgccggat gcaccgtgta ccacaaaccc gtattcgtag tcgcctgtga
     3841 tctggcggtc attgtgccac gtatggatca ggttccactc cgtcttgttg ttgtccttcg
     3901 cataagcgtg gaacgtgcgc acggcggagt catacaccag gcgcagatcg tagtcgacgt
     3961 tcgaaaaatc atttgtatcg gtaagtttcc agggcagcag catgttgatg gtgtccgagt
     4021 tgttcgtgac gcctcttacg ccgtaataag aaaatccgct gccatcatac ttgaactcca
     4081 ccgcatagaa gttgccattg ggaacacgcg ccatgatgcc ggcttcgccg ttcaatattg
     4141 cccggaaccg cgccgaaatg tcgaacgaat aacgcgccgt ctttgcgagg aaggtcgcat
     4201 tcaacttatt cctgtcggaa ggagcttcct tagaactgct gatcatgtat gcgttgttcg
     4261 cagtgacgta ggcgttacca tactgctggc cattgtaggt gcgctgcgtc caaccatcca
     4321 ccgtcagcgt ggctgcctcg gcagcgggga tcaactgcgt ggcgggaatc acctcgtcgg
     4381 gcaccacgcc gccccatttg agcacacagg aaacggggga gacttggagc cgcgcatcca
     4441 cagcgatatc atgctcaccg gcggtgagtt ccaccatgcc gctcgttgtc ttcgggccgt
     4501 ccgtccaagc attggccacg ctctttttgt cgatgtaaag tgcgccgccg tccgttgtca
     4561 caagcgtaaa cgtgtaggcc cccgtgaacg gcgtgaccag cttgccgcgc cacaaaaggc
     4621 gcatgttgtt cgtgatgctg ctgcccgcaa cgatttccac gcctttcgcc accgaaagat
     4681 tgaccgggcc ggtcggcagt tcgtaatact tttccggctg acacatcgcc gccacgttcg
     4741 cgacataggg ggcgtacacg gcaccgcgaa ggcccgtacc cgtaccaggc gcgtaatacg
     4801 tgtaagtaaa tggctcggag accgcctcca ccgcattaga cccaatcgaa accacgcggt
     4861 attcgtagat gttctgcgaa accgtgttgt cattacaggc gagcgaggtg cccgccaccg
     4921 tcgccaccgt gccccagacg ttcgtgccgt acacgcgccg ttccagacgg tattggacaa
     4981 cgttgatgcc cgcgttccag cccacatcca cagaaaggcc gtccgccgaa cgcgcgaaac
     5041 cgagtgtcgt aggcgccgtc accacgcccg ccgccacgag atcgctctgc gtccagccaa
     5101 agaacatcag ctccgccacg ttgccgccga attcgctcgg ccccgattcc ggcgcgtaaa
     5161 ggaaccactt gcgcgccccg ttgtcgggaa gcgacgtgcc catctgcgtg tagaacgtct
     5221 tgtcctgcga ggcctgcgtc accttctccg aacgttgcac cttgttcgtg agttccacat
     5281 cgccgtcgtc cgccgagaag agcgccgtgt tcttgatgcg cacatagcac cagttgtcgt
     5341 tgcggcagat gtagccgaaa ctcgtcaccc acgccggctc gccgaagtca agccccacgg
     5401 ggccgcggga ataggttccg ccactgaaga tatcggggaa cgtgtccggg ttgccgtcaa
     5461 acgccttcgg gtaatggtca ctgagataca cgccatttgt ggcgggcatg acggtcacgc
     5521 cgtcgagtaa tgtcgtctca tttccccaag agcggtccag cctgcggcaa cggcccatcg
     5581 tgacagcgtc cgatgtcgcg gattgccccg gaaagagcgg atgattacag tccacggtca
     5641 cgcggtagta ccccagcacg ccaacctttg ccgccgtgtt ggtgaacgtc gtgatggcaa
     5701 tcggcacatc cgtcgcgagt gccgtgaacg ggccggcgga accggtcgca tactcgaggg
     5761 tggctttcgt ggcatgaagc tcagccggca cggtccacgt aaggacggga tagaagttcg
     5821 tgacgtcgtc ataccccacc accgtaagca cgggcttaaa cggcacatcc gccggcacca
     5881 cctcgaactc gcacacgctt gaccggcagc cactcgctgt ccccgaaagg acacgtacac
     5941 tcttggccgt gacaggttca tcgaacacga tttcaatggg cactcccggc gttgtgtttg
     6001 cggacgacga cgtgaatacc gtcgtggcgt cagcaaacgt agcgtcactc gcaatctgca
     6061 cttttgcgcc gtagaagcgg ctgatcaggt ccggacgcga gatgacacgg atacggctga
     6121 tgcggatggc cgcgttataa tcatagccaa cccacggatt tgacggttcg tccacattcg
     6181 tatacatgtc gccgtcgacg gcgttaccga aggtcgtggc attgctgttc gtggcgcccg
     6241 tcacattcac gtaggccgtg aaatcaccgg ccgccaccca gtcactcgcc tctccgttca
     6301 aagtggtgcg catacgaaac gtggccgtac ccgcgaaatt cgttgcatag gtaaaatagg
     6361 cagcgtcata cttgttcgcc aggaacttgg cgagcgaatt gacagccttt gtgtaaatgg
     6421 tgacgtagtc gtcgtcggcc gcgccctgat ccttcatctg cacttccagc gtatgatcgg
     6481 taacaggcga gtatttcaca ccaaacgtga gtttgccgtt gaaaaaaccc gtcatcttga
     6541 gcgtgggggc cgtgggcacg gcaaaggccg cgcctacaat tccaagagcc gccgccaacg
     6601 cgacaacgaa cctgcgacct acagttgtac gagggcatgc ttttttcatg gtttcgactc
     6661 ctacgatttt cacgttatct taaggtcaac gaagatagta taccgcaccc tgtgacagat
     6721 cgtccagagg attcccgcaa aactggtaat cgcaatcggt tggtgacaga aggtgcgaag
     6781 atcatgcgtt tccggttccg ggtttatcgg aatcgggacc gctttcccgt attcgtcctc
     6841 gttctcgccg acttgcggcg tttgggttat ccggcagggc gacggatccg ccgttgcggc
     6901 cacaggatta gttcgcggcg gcgcggcgga attcgacttt acgcaggatg cggatctgga
     6961 gataggtgaa cccgatcagc gcgacaccca gaaaccacgc catggtggtg gccgtgctga
     7021 agcgcaggtt attgtacgcc tctttccaaa tggcgaggct gagcacgttg gtggcgtcgc
     7081 ccggcccgcc gaaggtaagc aggaagatgc tgcccatgcc ctgaaacgcg gcaatgaacg
     7141 cgcccacgaa attgatcacc atcaacggca gaatctgtgg cagggtgacg tggcggaagc
     7201 ggctgagaat ccccgcgccg tcaagagccg cagcctcgta atagtcgggc ggcagggagt
     7261 gcagggccgc cacatagatc aggctcgcca tgcccgcgcc cgcccacaca cccggcagga
     7321 tgcagcacac catcgccagt ccggggtctt gcagccacgt ctggcgcgag gcccccaacc
     7381 ccagcaggat gcggttgagc atgccgttct ccgtgggatc gtacatgagt ttccacagca
     7441 gcgcgatcac catggcgctg gtgaggtggg gcaggaaata gaccgtacgg aaaaacactt
     7501 tgccgcgcgg gatctccgcg agcatcagcg ccagcagcac gggcgtcaga aacccgagca
     7561 gcagggtgag gccgacatac cgcaaggtcc ggccccacgc gtcccaaaac cccgcgtccg
     7621 tggccaccag aatgaagttg tccaacccgg cccaacgggt ctcgcccacg atcttgtact
     7681 cctgaaacgc catcaccgcg ccgcgcagca acggataata actccacagc gcgacagaca
     7741 gcacagccgg cgccagcatg agccagggcg aaagcccatg ccgcacccgc atcgcgccga
     7801 tacggcgcgc ggatacggcg gaggcgtccg cgtcccgggc gtcccgccgc acgatcagcg
     7861 ccgcgcagcc cagcagcgtg atgattccca ccccgaacag aacgcgcgcg agaggacgcc
     7921 gacgcgccaa catcgtttcg gaggcccgga acatcagccc gcgattcgcc tccgcgttga
     7981 tctcccgcag ggccgccgca acatcgaacc gctcgccggt gtccgccagc agcaacccca
     8041 gaaaacgccg gtccagaagg tcggacaccg cctgccagaa tcccgcgaag ggctcggtac
     8101 gcgccagaat ctcacccgcc tccagccgtt cgtagttccg acggatgccc gcgggcacct
     8161 ccgcaaggta ctcatcgaat cccagccgcg tcaactccgt aggcgtgcac caacgcgcat
     8221 gcccttccag caccttctgc ctgaccgcct cgtcgttcac ctcctcgctg gccaactgct
     8281 ccacgcaggc ccacaccata tcgcgttcgg ctttgggacg gcggcccacg ccttcggtca
     8341 tgctgtagaa atgcttgtgc gcctgaaaaa caggcttgag tcccggccgc gccgccggaa
     8401 agggcatgat gcccaccatg tccggcggca gattcagatc gcgcgtcaat ttttcgacca
     8461 gctcggcacc ggagaaaagc gccacgacct cgccttgggc gaagagctgc ggcagatcct
     8521 gagtgagccg cggcagcgcg cgcaccacgc cccggatgac ctccccaggc tgaaagcgat
     8581 gttcccgccc gtccggcaac gtcacgcaac ccgctgcgag cgcttcgggc gacagatcaa
     8641 ccggctcgcc gctttgcgga tcacgcagcc agggagccca ggccaaccga tggaagaact
     8701 cggccgcctc aatcgcctcg ggcgagtcga acgccgcctg ccacacgccc ggcacaccgg
     8761 agagatcctc gcctgtgtcg ggcgcgatga accgcgtctc ttccatgggg aaacggtagg
     8821 tacggcccgt cgcgggcgag acgcggaggt tgacgatcgg cgagccgcct gcggcctgca
     8881 cccaaggcaa ccagccccac ggacggttct ccgcgccgaa cgcacgttgc ccgcgctgca
     8941 ctcgggcgcc cgcaatacgt ttgccgggga aggtcagacg ctggcaccag cggaaaaact
     9001 cgtcccacgt ggcgggcacg ctttccggat ccacaccggc ctgctgcacc agatccttgc
     9061 ggtacacgat gccgtagtac cagatgccgg ccatcggcag accgtatacc ttgccgtcgc
     9121 gcgtggcggc ctgacgccac agcggcggca cgcgcttcca gcccggccag cgcgcctcgt
     9181 ggtcgccaag ctgcccatcg ccgtccaagt cctccccgag ccactcgttc agtggatgga
     9241 ggaaaccctg ctcgatatca tgccggatga tgtggaacca gcaaaagtag atgtccggcg
     9301 ccgtgccgcc cgccagcgag agcatgaacg gcgcgcgccc gcccgcgccg ggcagttcaa
     9361 gaccacccca cttttgcggg ttgagccgcg gctcgcgctg cgcgaaacgc agaatctgcc
     9421 gggtccccgg attctcggga cggtccggat caaagccctg caggaacgtc accgtcaccg
     9481 gttcgccggc ccacacacgg aaacccacac aaaggctcgc gaaaatgaat cctgaacgcc
     9541 caaagagcat acgcgaacct taccgcaccc ttggcgcgaa agcaagcggg catgtaaacc
     9601 caacaatcgc gttcgtgacc atgaaacaga tgagccttca tgaaaacccg gttccctgtc
     9661 ctcgtactcg ccgacttgcg acccgcggca ttcgacctgc ggcgcttggg ttgtccggca
     9721 gggcgacgga tccgccgttg cgggtggaga cacccgtgtt cccaactccc gcacgaatac
     9781 acgaatgaac tctcatcggt cagaaaacgg gggtgcaacg cgggttcata aaacacgaac
     9841 ggttttcgct tgcggcacgc gtgggcttca ggtatattgt ttctttcaac agaaacgatt
     9901 ggcccggaca ccgggcctta tcagggagac acgcatgaac gttccgcgcc ctttgtcagg
     9961 caatcccgac gccaaaggct tttttggcaa atatggcggc caattccttc cgccgcagct
    10021 cgaaaaaccc tttgcggaga tcaccgaggc ctaccaccgc atcgccaacg acgcggcgtt
    10081 catctccgag ctgcgcttga tccgcaaaca cttccaaggc cgtcccacac cggtgtacca
    10141 tgcgcgcaag ctctcactcc agaacggcgg ggcgcatctt tacctcaagc gcgaggatct
    10201 caaccacacc ggcgcgcata agatcaacca ctgtatgggc gaggggttgc tggcgcgctt
    10261 catgggcaag aaaaaactca tcgcggaaac cggcgcgggc cagcacggcg tggcgctggc
    10321 cacggccgcg gcttatttcg gtctgcaatg cgagatccac atgggcgagg tggacatcgc
    10381 caaacaggcg cccaatgtca gccgcatgaa attgctgggc gcgaaagtcg tgccggtcac
    10441 ccatggcctc aaaaccctca aagaggcggt ggattcggct tttgaggcct atctggccga
    10501 ttacgaaaac tccatctact gcatcggttc ggttgtcggc ccgcacccct tcccgttgat
    10561 ggtgcgtgat ttccagaaag tggtcggcat cgaggcccgt gagcagttca tcgggatgac
    10621 cggcaacctg cctgacgcgg tctgcgcctg tgtgggcggc ggttctaacg cgatggggat
    10681 tttccacggc tttctggatg acccctgcga aatctacggg gtcgagccgt tgggacgcgg
    10741 cccgaagacg ggtgaccacg cggcgacgat gtcgtacggt cgcgaaggga tcatccacgg
    10801 gttcaagtgc tacctgctgc agaacgagga cggttcggcc gccccggtct attcctgcgc
    10861 gagcggactg gactatccgg gcgtggggcc ggagcattgt catttgaagg ataccggacg
    10921 cgtgcgatac gtgacctgtt ccgacgaggc gtgccgcgat gcctttttca agttgtgccg
    10981 actcgagggg atcattccgg cgctcgaaag cgcgcacgcc gtggcgttcg cgctgaccca
    11041 ggcgcgcgag atggaaagcg gggcgattct ggtcaatctc agcggacgcg gcgacaagga
    11101 cctcgattac gtgatcgaga attggggcgt cggcgaaaca gaggcgtaag cttccggcac
    11161 gggcggcgat ccggcgtgaa gaatccctag ggcgttttcg caaaaggtgt agccgttccg
    11221 ggtaaggacg cctccccgag gcgtccgcgg acggttcggg gaaccgcccc tgccagcaat
    11281 cgaacctcgg ccacaaaaca gcgtaacctg cgctaccgcc gccagcaact ggggaacagc
    11341 agaaccagca ccgtgtacag ctcgaggcgc ccgagcagca tgcaggtggc cagtaccccc
    11401 tgtcctgcca gcggaatcga tgcgtagttg cgcaccggac cgaccgcgct gagacccgga
    11461 ccgacattgc tgagcgcggt gatcacggat gttgtggctg tctcgaggtc cggggtgaag
    11521 aaggtcatca aaaacgagcc cagcgcaaac accgcaaaat agataatcac gaaggctgac
    11581 acatgcagca ccagatccgg ctccaccggt ttcgggccga gtttcacttg gatcgctgct
    11641 gtcgggcgca tgaaaagccg gatctcgcgc aggattttct tgaagacgat catgatccgg
    11701 accaccttga tccccccgcc cgtggagcct gcgcaggcac ccatgagcat catgaacacc
    11761 agcaacagac gcgatgcgcc cggccagcgg tcgaagtcaa ccgtgcaaaa cccggtcgag
    11821 gtcagaaccg aagtgccggt aaaaaaggcg tcgcgcaaac accgtgcgaa ggagccgtgt
    11881 cccgtggccc agatgttggc ggtcataaaa aggcagcacc ccgtcagaaa cagaaaatag
    11941 gcacggcact ccggatcgcg gacatagtgg cccggccgcc cggtgagcgc ggaaagatgc
    12001 agcgaaaaat tgatgcccgc cagaaacatg aagagcgtga taatcccttc caccgtcgcg
    12061 ctgtcatagg ccgccacact ggcggtacgc gtcgaaaacc cgccgctcgc gacagtcgcg
    12121 aacgtgtggc agacggaatc gaaccatccc atgcccccga cccccttcag caacagcgtc
    12181 tgcacgaccg tcagcaggag atacacggcc cacagcagtt tggccgtggt ggcgatgtgc
    12241 ggtgtcagcc ggtctttgaa gggcccgggc atctcggctc ggtacagttg catgccgccc
    12301 acgccgagaa agggcaggat cgccacacag agcaccagca cccccatacc gccaagccat
    12361 tgcgtcagcg cgcgccagaa caggatcccg cgcggcaggt tctcaagatt gtccagcact
    12421 gacgcgccgg tggtggtaaa gcccgagacc gtctcgaaca gcgccgagac cggatgcgtg
    12481 atcacgcccg taagcagata cggcaacgcg ccgaacaagg ccgccgacaa ccagccgagg
    12541 gtcaccacac cgaacccgtc ccggcgtccc agattgaggt cgccgcgcgt caacatccct
    12601 gccaccagcg aaacggctag ggtgatccct ccagccagaa gaaacgcgca ccgcacctcc
    12661 agcgcgtcac cacatacgtg ggagaccgcg gcacaggcgg tgaccgccaa cccgatgaac
    12721 agggtcatgt aggaaaccag atggaatacc gctcgtttgt tcatacgcgc gcgatcattt
    12781 gcggaacatc gcctgaatct tcttcaccgc cgccgggtcg gcgaaaatca acaggcggtc
    12841 tccagcctcg aaacacagcc caccggtcgc ggggagcacg ccgtcgccgc gctggaccac
    12901 cgccacgatc gaccccgacg ggaaccggat gtcactgacc ctcttgcccg cataccgact
    12961 ggtgggggac aactcgattt ccagcagttc cccggggaga tcatgcagca gtgccgccgc
    13021 gcgcacatcc cggcttctca ggtaatgcag aatggcattc gtgatcgact cgtaggggct
    13081 gatgatccgg tccaccagcc cgaggttttc catgatcgga atgtagtcgg tgcgggcgat
    13141 ctgagtgacc acgaaatccg ctcccttttt gcgggccatc agacagttca tcgcgttgtc
    13201 ctcatcatca ccggtcacgg ccacgaaagc ggtcccggga acgatgcccg tctcatccag
    13261 tgccgagccg gacaacgcat cggcccgcaa cacgagcgta tggtccaact cgccggaaca
    13321 gaagagcgcc cgctcctcat cctgctccag cagcacgaca tgaagaccaa tgcgctccaa
    13381 attcttggcg atggccaggc cgatatcccc cccacccgcc acaatcactt tctcaaacgc
    13441 ctcccgaacc ggatccatcc aggttgagaa tacccgaatg tcatccggtg cgccgaccag
    13501 atagagcaca tcctccggca gaaatgcggt atccccccgg ggaatgacca gacggccctg
    13561 acgccggata gcgatgatac gcagcttgcg caccaagtcc tgatccggca acgtagcggg
    13621 cgtgtgcccg agaatcgggc ttccgccgac gatgccgaaa ccggccacca ggacacggcc
    13681 gccgaacagg tcaaaggact ccaccgcccc aggcagcaac agggcattac agatttcctt
    13741 tgcgcaggcg aatttctggt tgatcagcag atcgatcccc agtttcgtca ggttgtactt
    13801 gactgcaccg ggatcatact gcgcgctccc cacccgcgcg atcttgcgcg gcaccccttc
    13861 cgaatgggct aacaaacacg cgatgatatt gatctcatcc cgatccgtca ccgccacaaa
    13921 caggtcggcc ctccccgccc cggccttttc gagaatcgcg ggatccgccc cggacccgca
    13981 gacggtgagc acatccagcg aggcttcggc ctgctccagc gcctcagccg actcgtcgac
    14041 catcaccaca ctatgacgct cccgacagag atgagacgcc aacaaccgcc ccgcatgccc
    14101 ggcaccgatg attacgctat tcatgttgcc cctttacaca ccctgtatat tacgagatgc
    14161 ggcgcgttac acaaacccaa aaacacaatc ccccctttac cgctcaccga ctggccaccg
    14221 cgcccggcat gctgccacac gctgcccggt aatgggcggc gacctcttcg gctgtcaatg
    14281 actatatcag atcgcggacc tttttccaag gagactcgcc ggatgacgaa ggcaatgcat
    14341 ttcccgtctc ccatctcccg tccccattgt tttgactggc gcgacatgtc ataatcatgg
    14401 gcaccatagc ccaaagaaag gaacagtcat gaacaagacc tccggaacgg gatggcgtgc
    14461 ggtcctgtgt gcgttgtgta cggtgtgggg tgcgggtgtc gcggatgcgc agcacgtgac
    14521 cgggcagacc aaaccggttt tgcctgtggt ggatctgtcc ggagagacgg cgcggcatgt
    14581 ggtgatcgcg gcaggaacgc cggaggagta tcagggacat ccgaccacgg tcctcttacc
    14641 ggacggcaag acggtgttct gcgtgtggtg catcaatcac ggcggcgcgg caggccccat
    14701 ggcgcgcagt gccgatggcg gaaaaacatg ggtgcggctg gacaccacgc tgccaccggg
    14761 tttccggacg catcagaact gtcccagcat ctaccggctg atcgacccgc agggcaaggc
    14821 gcggctctgg gtgtggtcag cggccaaaga cacgcggcag ggcacgccga tgcccagcat
    14881 catgagcgag gatgacgggg agatgtggcg ggagatgccg ccgctgggcg aagctttccg
    14941 ctgtgtgatg accttcagca gtatggttag attgaaggac ggcagttatc tggggctata
    15001 ccacaggggt gagggcaagg gcgaagggcg caagttcctt gaggtcgtgc aaagcgtgac
    15061 ccgagacggc ggattcactt ggagcgtgcc gcgcgttgtg gccaatgttg cagggaagat
    15121 ggcgtgcgag cccttcgtgt ttcgctcgcc ggacgggagc gaactgtgct gtctgatgcg
    15181 ggaaaacacg cataaggggc acagtttgca gatgttcagc cgcgatgagg gcgcgacttg
    15241 gagcgtgccg gaagatacgc cgtggggatt gaccggtgac cggcacatgg gcgtgtacgc
    15301 tccggacggg cgactggtga tcgccttccg cgaccaggca cccgaatcac cgacaaaagg
    15361 ccattttgtg gcttgggtcg gaaactacgg ggacatccgc cagaggcggg acggccagta
    15421 caggatcaag ctcttgcacg ggtataaacg ggttgacctc ggttatcccg gcgtggaacg
    15481 gctggctgac ggcacgcttc tggcgaccac ctatctcaag tactgggagg atacgcgcca
    15541 acactccgtg gtctgcacgc gtttcacgct ggcggagacg gacgcgaaag cgggcttgaa
    15601 cccctgagta cggcagggat cgcgttgtgc tgtgcggatg ccggagcgga tcacctgatc
    15661 cgaatctgcg tgccgggcgg cgcgtagtaa agctccagat ggtcggggaa ccttcccaca
    15721 tgccacccgt ccggaagccc tgtgacggat gcgaatgaac cggagagcgt tccggtccat
    15781 gtcgccagcg tgtaggtctg gccgcggacc agcgaatccg gctggagggt gagggtcacc
    15841 ccctcgccga tcgtcagatt accggtcacg gccaacgtgc cgctgccgga agcgcctgcc
    15901 gccaccacca atgcgttggt cagcgtggtg tcgcccatga tcatcagtgt accgtccaac
    15961 accgtatggg gagccgtgtt ctgcacggat ccggtgagga tcaccttccc ggggccggtc
    16021 ttgatgattc cgccgggtcc ggtgatcgca cccgagaagg tcacgccgct gtcatccgtg
    16081 ttggccgcgt gcaggcacac gtcgcgcgtc agggcgaggt cgccggtgaa ggctgacgcg
    16141 ccggttgtct gattgccacc cagcgtcatg agcgcgcccg gcgcgtagtc catcaggatg
    16201 atgccgttcc ccacggtgta cgggccgtcc gtgagcaggg acagcgcgtc gcccggtgcg
    16261 gttccggcgt cgctcaggat cacccgttcc gtgcccagcg cgcccaccgt gccattgccg
    16321 ggcgagtccg cgccgaccag gattgtgcct ttgcggaggg tgacgctggt ggtcatggcg
    16381 tttgtggtac cggagatacg ccaggtgttg ttcccggctt tgacaaagcg gaaggccccg
    16441 cggttggaag agccccaggt tccccagaga tacccttcga attcgaagac cccgttgtca
    16501 tcgcaaccga tcagggccac attgtcggaa tcgctgttgg aaatgccgcg gccgccaaag
    16561 gcgccgctcc cggtcacccg tccgaaatgg agcggataat gcgaagggta gcaagaggtg
    16621 aaactgtaca cgcgatgctc cggcacaacc agttcgccgt tcggcaggcc gccgccgtac
    16681 gtggcgtcgg ccgccatcag gcccgacgcg tcgaagcgcc cggtgaacgc atggttgagc
    16741 aggttgtaga aacggaagga agcgccggtg gccgaagagt tggtgatcgt cagcgtgccc
    16801 ggcccctcca gccaatccga ggtgcgggtt aacccgtcaa agaggctcca gcccatgttg
    16861 acggtgatgc cgttgagatc aacgatcgca ccgcccgcat cgatatggag gactttgcgg
    16921 atggcgacat cctcggggtt gccgcctccg ttacccccgc cattgttgcc gtacaggaac
    16981 ccgccgcctt tgagcaccgc gttgttgcgg acggtcatgg acatcgaccc ctcacccaag
    17041 aacgagttgg cggaggtcgt tcccgcacgg tgctcgatcg agccctcctc cacatcaaaa
    17101 aacatcgtgt actcccgcgc ggtgacggta tcttccgccc ggtccgcgcc agagccgaag
    17161 ccccggccga caaaccatgt tcccgcgccg cgcttgacca ggcgtcctcc gttccgcaaa
    17221 acaggagtgg ggttcgcgtc aagattggcg acccggtccc ccgcgccgct gacgttgagc
    17281 acgccgcccg cgctgttgat gtcaagcgtg gtgatattcg tatagccggg gtacacctca
    17341 cgggcgaaat cgaaactgcc ggtcgtggtc agcgtgccgc cattataaat catgacgaag
    17401 ttctgcacac tcttccagga gccgtgccaa gagccgacac ccagacactc ggggccgctg
    17461 atctccagtt cgccttcaag aaccgaggtg taatcatcat gccggttgtc ggcgttgccg
    17521 aggatgactt tgccggggcc ggccttcatc acgccgacat ccgaaagggt gatgccggtg
    17581 acctgtccca gatcgttggt gttgaagacc ggaaaaccta ggagttggct gtcgatccgg
    17641 accacagcct gggtgttgaa gttgtaaatg atgaggttac cggaacccat gcgggcgatc
    17701 gcgccgcccg tgatatggac cggggtgttc cccatcgctt cggtcaccag gatcgcgccg
    17761 ctcttgatct gattggtgcc ggacagattg aggataatcg gattcccgtt gttgggggtg
    17821 ttgaagcgca gggtggcgac ttccagattc gcgggaacgc tttgcgccag accggcggtc
    17881 acgtccacat gcttgttcga gccgctccag tcggacgcgt agtccgtgaa attgctgatg
    17941 gcgttgccgc tcgcggtccg cacggcccaa tccctgccct taaaaaggat gttggcaggc
    18001 aggatgttgg gataggccga gttttcggac ccgttggagg tctggatgag cttgtagggc
    18061 ccctccccct ccagattcag gacagagtcg gtattgggtg tcaagaagtt gaaggccagc
    18121 ggcatggcgg aatcatggat aacccgcgcg ctggcccctt tgtaggtgga gagcgtccgg
    18181 accgcctgat ccggcgtggc agtctggccg ttccggagga tcaggcgcgc gttgttcccg
    18241 agcgtgatgg tcccgtccgc gggcgttttc tggccgcccg cttcgctgta gtccagaatc
    18301 aaatcgccaa gcaccacggc ggtcgttcca gtatatgcgt tgtcctgctt gaggatcagc
    18361 gcgccgaggc cgcttttggt caaaccggag gtaaaaccgg tcagcgcatg gcccagggtg
    18421 aaatcggcgc cgtccgtgag gagctgtacc ggtttgctgt tggtggatac cagcgtgaag
    18481 gcggaggggt cgacgccggt ggaggagtcg atgcggaacg cgccgccgtt gaagatcagg
    18541 gggccgctgc ccaggcttga gggggtgagc gcggtcagga acccgctggt cagcagcgtg
    18601 ccgccgctgt aggtgttatt gcccgcgagc accagattgc gcaaagagac gttttccagt
    18661 cggcagccgc gtccgccgtc cgtgacggct ccgtcgatgc gcaggttccc gccttcagac
    18721 cggggatacc ggctgaccgt gcccgccacg gcggcatcgg aaacgagcgt cacggggccg
    18781 gaccagaccg aggtcacgcc cggcgactgg caaccgctgg tcaacgcgcc cataccgttc
    18841 gcgccccgcc cgccgatcag gatcggtttc tcgggcccga aatggaagcc gttgacatca
    18901 acggtggcta ccccgctggt ggcgatcagg gtgttgtcga cgccgaactg attccccggc
    18961 acgttgtagc ggaagggatc cccggcccgg acaatcccct tttccacgat caggtcgccg
    19021 gaaaaggtgt tgttgccctt gagcatcagt tgcatgtcct ctttgtcgaa gcggacccgg
    19081 atgccgcggc gtccgccctc ctcgcggata tccctgccga gccagactgc ggtgagcggg
    19141 ttattccagt tcccggatgc gcggccgttg tgctgcacct ccagatcgga tttgagcaag
    19201 agaggaatgt tgatggcata ccggaaatta ctggtcagga ggggcaggca gcggattgtg
    19261 gcgggctcgc cttccacccc gctgtcgagg gtaagatagt tagtgaagac accgaaccgg
    19321 taggtgccgt aaaccgggtc cgggttaccg gatgtccggt tttcccacgg aagggaaacg
    19381 gtgaacacgg aggtcaatcc ggcgacgggc gtgtcggcgg gcgtgacgat atgcccgagc
    19441 acggcattgg tgaagaagtt gttaagcgtg taattgcctt tgacgtacag cgtggcgccc
    19501 gcgcctccgg ggatcacgcc gtcaacccag gttgcgggag tggaaaaatt tccggtctgc
    19561 tgcaccgtcc cgtcttccgc ccggatcaag cctcctgcga aaaacccgat cacacacaag
    19621 acgtggatta aattggggat tgcatatctc atcacatctc cttttttacc tgttccgcgt
    19681 aaaatccaaa aatccaacgg ctgaatttta aaaagtgtgg ccattgcgtt gtcctttcgt
    19741 ttgtgtgact cagttcgaca acatgctgtc
//
LOCUS       DAOSLE010000040        18939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3144_length_18939_cov_7.925863, whole genome shotgun sequence.
ACCESSION   DAOSLE010000040 DAOSLE010000000
VERSION     DAOSLE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18939
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3144_length_18939_cov_7.925863"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1781)
                     /locus_tag="PK576_04355"
     CDS             complement(177..1781)
                     /locus_tag="PK576_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HOE60855.1"
                     /translation="MRLGKAWEALLCIGVLAGALALRLPHLAQRPMHGDEANQAVRTG
                     QLLEEGVYHYDPTDHHGPSLYFAAWFFCRATARTFADTTEWSYRLVPVTFSLLTLLIM
                     FAMGVSERQGLFVNRSARFTALLGMAVSPAMVYYSRFFIQETMLVTFLAGMLLCAVRY
                     AGLRAAEVPGERRSGRRTLWAAAGFGAFAGLAAATKETAVLSFAAAAVATVAACGIGR
                     LRRVWRTRDAVLAAGVAIGVAALLFSSFFTYPRGIVDALFSTAQAYLKRATAVPEHQH
                     PWNFYLKILFWFRYGRGPLWSEAALLVPALIATVTAFLRFPDASEEETRVLRWTRFLA
                     VYTLVLTALYSAIPYKTPWCALSFLHGVILLAGMGVGQAVAWLARQNIRGVRLAGTVV
                     VWALVAFVAARHAAQAYRACFRLPADPRNPYVYAHTGTDALNLVAAIEEAARAAQGTD
                     TPIAVAVPVPDTWPLPWYLRAYTRVGYWTRIEQIPESFHPVVVIAAADQGDVADARFG
                     TGRRASFYGIRPGVLLNLFVPVPSAP"
     gene            complement(1792..5946)
                     /locus_tag="PK576_04360"
     CDS             complement(1792..5946)
                     /locus_tag="PK576_04360"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOE60856.1"
                     /translation="MMKNRFSGSGPNLAFFRRIDWTAFWTATIISFLVYFFTLGPSVT
                     LEDSGELAVAGDHLGVPHPPGYPIWTMCAYLFARVFSWVTFRGQPTPAWSISLMSAVF
                     AALAAGITAMLITRSASDMMRDAHEGEEDFRQQESDLLCWAGGVGGSLVFAFSPVMWS
                     QATIVEVYTLNAFFLMWIFLLTYRWMRCPAEKTLWLTAFVFGLGLTNYQVLLLAALPL
                     VVMIFLRDIALFRDFLLVGIPMLLTSHLLQIGAMVPASPGMGGPAFAKAEPLSKVSVP
                     SPGLLVAGCVCVLLGLAVGHGLSWRRNAVSKREPGTGKGGRLDALALQGAWITAGVTI
                     LGALLIFFSTATTRMKEVAATDAPLFDPSVYTSVAALAVLVLAFSLGGAYVFKSRWGE
                     RETLPWLIAAGVSLLLLFFRLSAIRGAAMPAGYMGQPYNWSLPTLVLAGGLVVLFLLA
                     VTLPRGIFYALPVAAVQLAVFVLLRKGGMNGLTHPTSWWFWWPVIWNFVVIGLAWQLL
                     PNGRAVAVTTLFAELGVSFYIYMPIVSDLRNPPMNWGYPRTWEGFKHAITRGQYEKLA
                     PSDVFTKKFLFQLGTYFTDLRVQFTLIAATLGFLPFTLWSFRVGKRRIRAGYVASGLY
                     LLTCVLVLISEFAGGEPLWRLDKLLIGLILFLLVVGGATVVLTQVEAFVKRTWQARNL
                     SEGLTVGISLCGMLAVIALFLAKGLTHALSGTGDPGIGVRLASLALALVILAACGAGW
                     FFFRSFAEKRFDFRSDMDQVTQQWMIATAAGFLVMSVLLVVLANIKGDLQDAFIQKVK
                     FISSHGLFAIWIGYGLAFGLTFGNRILKWLVRLGWLNARLLKPLRIGLVCAGVLVAAI
                     PIYENYTNAYLVFSMSGAEQNGHDFGWQFGNYQLRGAEAITEELEADEEPLPNPLFPP
                     AMEPNAIFFGGTDPGRFVPTYMIYSAQVRPDVFLITQNALADNTYMSTMRDLYGNQIW
                     IPTPDDSARAFQVYVDEVQAGKRPKNAALTIENGRVQVSGALGVMEINGILCDMIFAK
                     NKARHAFYVEESYVINWMFPFMTPHGLILKINPERQAIPAETVRADLDFWDWYVRRLL
                     RNPLFRRDLPGQKSFSKLRSAIAGLYAAHGLRAEAERAFQEARTLYPVSPEANFRLVQ
                     EVLLPQERYVESIRILEEYNRRDPNNERGYGFVDFIRRILNTEAKVRALTTKMQQSQT
                     LQPDEVFELALAYRDLGQNDAAAKYLEQLMTVPNLPVTRMFEIGSLLSGLKRGESACK
                     AMDIVMARMPENMPPEQLLEVVRVYGEAGNADKMAAPLSRYLQLRPNDWQAWLDMATL
                     YAMKRQQQPMQFALQKAINLGKMQAVQRIQENTILRQVAGPLLQQMMQQQGPAGLPPF
                     GPVRGR"
     gene            6132..6800
                     /locus_tag="PK576_04365"
     CDS             6132..6800
                     /locus_tag="PK576_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE60857.1"
                     /translation="MPIQVIIVEDDPRLRSGLREIINSDPACACVGAYGSGEEALQQA
                     PALNPQVAVMDINLPGMDGIACVRHLAEILPELQIVMLTVYQDTDNLFRALAAGAHGY
                     LVKPARARDLLDAIRDIHRGGAPMTSALARKVVDAFRDRASPAQPPPAATPPTTESDE
                     LAPREKEVLDLLAQGFSYKEIADKLNVAFATVHTYVLRIYKKLHVRSKNAAVARWLGY
                     QPGE"
     gene            6816..8066
                     /locus_tag="PK576_04370"
     CDS             6816..8066
                     /locus_tag="PK576_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60858.1"
                     /translation="MTTPRPFGRRAFLRLAHAGMAVLPVMTAKRVSGQSAAVQPATRT
                     YARQVKLGLVGCGGRGSWISNLFKANGGFVMTAVADYFADRAMRVGAQLGVPPSRCFS
                     GLDGYRRLMASGIEAVVLQTPPYFFPAHAEAALDAGLHLYMAKPVAIDIPGTLTIQRL
                     AARATQERRVFLADYQLPRDPVNQEVAKRIRAGALGRLQTVFSSGWAGGNGYQDPPLE
                     TPIANRLKDLVWCNDLALGGDYLVHYDVHILDAVVWALGKKPVAAMGASAALRPSVHG
                     DARDSSTVVFLFDDGTIWCHQGMLGSYHDWLKVGRLTASLQGTQGAACLSYAGKAYVR
                     GGPRHFSGGIPNPEASVKTNIAEFRQAIETSACDNAQVLRAVDSNLTAILGREAAARR
                     DRVTLDTLMREARVLQPDLSGLIR"
     gene            complement(8088..8240)
                     /locus_tag="PK576_04375"
     CDS             complement(8088..8240)
                     /locus_tag="PK576_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60859.1"
                     /translation="MQREWVGNARLITRKLGLSGFTVFNYDTHAEAVLPYLRLGVSAP
                     TGWWPW"
     gene            complement(8305..8559)
                     /locus_tag="PK576_04380"
     CDS             complement(8305..8559)
                     /locus_tag="PK576_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60860.1"
                     /translation="MKIVRIVFLLVAGIGGARAETIPAPPKGEAVFPAWQAVRDTKTL
                     RALCVPMSGTPQASWEEAGFARPPVRVWRRERRPQDRKRA"
     gene            8694..10811
                     /gene="uvrB"
                     /locus_tag="PK576_04385"
     CDS             8694..10811
                     /gene="uvrB"
                     /locus_tag="PK576_04385"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOE60861.1"
                     /translation="MEQDITRFNLVSAYQPAGDQPEAIAALSQGLRAGARRLTLEGVT
                     GSGKTFTMANLIREWGRPTLVLSHNKTLAAQLFAELKGFFPDHAVEYFVSYYDYYQPE
                     AYIPQTDTYIEKDASINEEIERYRLGATNALLGRRDVVIVASVSCIYGLGSPEDYREM
                     LVDLTSGCSVARDAVLEQLVGIQYVRNDYEPQPGTFRVRGDSVDIFPSYETDGLRVTF
                     FGDEVESLHTLDPVTGRTGSPLSRVVISPAKHFVMPRAKIDAATSRIACELDARLAEL
                     ERQGKLLEAQRLRQRTDFDLEMLRELGYCNGIENYSRHLAGRVAGEPPACLLDYFEGE
                     FLTILDESHVTVPQLRAMYNGDQARKQTLVEHGFRLPSAQDNRPLRFEEFLDKVGPIV
                     FASATPGPYERQVSEITVEQVIRPTGLLDPPVEIRPLDNQIDNLIEEIRRAAEQGDRV
                     LVTTLTKRSAEDLTAYLTEIGIRVEYLHSDIGAIDRVAILGRLRTGAFDCLIGINLLR
                     EGLDLPEVALVAVLDADKEGFLRSATSLIQTAGRTARHVNGRVILYADRETEAIRKMV
                     DITRTRRQRQEAFNATHGITPQSVRKALNESLDLYAQARHIEERMFAAESSESYDLCR
                     AIADTEAEMIAAADALEFERAALLRDELRELKHLLATPAGPETDGAKSTKPKNRRTRR
                     PQGKGKAAAPPQPAGKRSVRRRP"
     gene            complement(10829..12007)
                     /locus_tag="PK576_04390"
     CDS             complement(10829..12007)
                     /locus_tag="PK576_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014280587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE60862.1"
                     /translation="MTTVSKSAHDFVSPLVRDLPKSGIRDFFDIVASRKDVISLGIGE
                     PDFQTPWHIREKAITSLERGMTRYTSNLGMPELRQELAGYMRDTFRAEYDWQTEILVT
                     VGVSEGFDIAMRAVLSPGDEVLYHEPCFVAYAPLIRMAQGVPVAVETFRRDEFRLTVE
                     ELEKKVTPRTRVLLVSFPNNPTGATLRREDTEALADFVIRHDLLLVSDEVYSELTYEG
                     GRHSLAAIPRLRDRLIFLNGFSKAWSMTGFRLGYVCAPAPLTDAMMKIHQYCMMCASS
                     TAQMAGIEALQHGAADVAAMRESYHLRRNYIVASLNEMGLDCFMPRGAFYVFPSIQST
                     GLDSTTFALRLLNECNVACIPGTAFGACGAGFLRCAYATGMEQLKEAMDRMAAFVQRL
                     "
     gene            complement(11979..12458)
                     /locus_tag="PK576_04395"
     CDS             complement(11979..12458)
                     /locus_tag="PK576_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE60863.1"
                     /translation="MDTLLELLKKNARESDATLAKKLALTETEVRERIAAYERDGVIR
                     GYQAVLNQDRIEQTDVRAVIELRIRPEREGGFDRIANRIGKFPQVESMCLMSGAYDLL
                     LFVKGQSLQQVAQFVSAHLASLDGVLSTTTHFILKTYKDQGVLMEGVSHDDRLQVCP"
     gene            complement(12513..12995)
                     /locus_tag="PK576_04400"
     CDS             complement(12513..12995)
                     /locus_tag="PK576_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HOE60864.1"
                     /translation="MKNWKVLFVKPRTEKKVTEYCRLYGIPFYLPLRERQRIVQRRKI
                     QVRVPIFPGYVFACFPDAQRLALLQTNLLVRILVPINARRLLRELIMVRRALRANPAL
                     KAGPPLTEGRMVRILSGPFMGIEGRVKRLAGTVRVVLNIELIGQAVTVTTDCEQIEPL
                     "
     gene            complement(13008..15524)
                     /gene="gyrA"
                     /locus_tag="PK576_04405"
     CDS             complement(13008..15524)
                     /gene="gyrA"
                     /locus_tag="PK576_04405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOE60865.1"
                     /translation="MSDGIQPDQQTDQGLQNINIEDEMSSSYIDYSMSVIIGRALPDA
                     RDGLKPVHRRVLYAMNELGNTSNKPYKKSARVVGDVIGKYHPHGDQAVYDTIVRMAQE
                     FSMRYPLVDGQGNFGSIDGDPAAAMRYTEVRMDRLAEEMLDDLEKETVDFKPNYNEEF
                     QEPTVLPARLPNLLLNGSTGIAVGMATNIPPHNLGELVDGLTHYIENPECTIDELMQF
                     VKGPDFPTGAILCGTRGIEAMYKLGRGSLTIRGKAEIVEYKNKEAILITEIPYTVNKA
                     SMIEKMADLVNGKVLEGISDIRDESSSDGIRVVVELKRGAMAQVVLNNLYKHTQLQTT
                     FGANMLAIDQGRPKVMTLKDLFRSYTDHRFEVITRRTQFDLAKAEARKHILDGLLVAL
                     DHLDEVVALIRGSSNREEAKVRLVERFAFSDRQVDAILEMRLYQLTGLERDKVEAESR
                     ELCDRIAYLQELLRDPAKIYGLIKQDLAELKAQYVLHARRNDKRRTEIVPLEGEVNIE
                     DLIADEPCVITLSLRGYVKRVPLTVYREQRRGGRGITGVAIKEEDLITTVFVAETHDT
                     LLFFTNFGRVFAERAFEISEAPRTSFGKPIVNLLQFQEGEQVAALLPIRAFAEDVDVM
                     FATEGGTVKKTALVDYRNINRNGIRALNIGEHDRLIQVRLTRSGDDVLLITANGQAMR
                     FNEKQVRRMGRAATGVRGIRLGAGDAVRSFDVVDDQATLLIATENGYGKRTMFSEFTP
                     HNRGGKGMIAIKDVAGRNGKVVAAHAVREEQSFISITSAGMMIRSPVNDVRLCGRTAQ
                     GVRLVRLDDGDTLVSVSIADADTDADIDTDTDADTEETQG"
     gene            15667..16632
                     /locus_tag="PK576_04410"
     CDS             15667..16632
                     /locus_tag="PK576_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60866.1"
                     /translation="MDNRTPCPICRTPNISYASAGANTSEINCPRCGRYQIQAFAETQ
                     LHNSPPAFPPAHLLSGLCRNTWDHLGEKVLITVDLFKSWADLERVAKLPVPRDTDVVT
                     KGEYLLKHLRRRSKTLADRVSFTPHELACGFCSTKNELLFCLRYLADRGWVEECSAAN
                     RKGPPRDAITFRLTPAGWAALAAPATPAQPRAVVSVMLDREDESLWTQGFAAAAQAAG
                     YVAERVENREQLNKINDELIVSLRRAAFVIADLTGQAPMTYFVAGFAGGLGKPVFWTC
                     EESEARDKRLWLETRQSIITTWTRDKPEVFAARLAQRIEATIPGE"
     gene            complement(16651..17550)
                     /locus_tag="PK576_04415"
     CDS             complement(16651..17550)
                     /locus_tag="PK576_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE60867.1"
                     /translation="MSQSKSIRWLGFLTLGVNISLMLVKITVGLMGNCYALIADGVES
                     LGDIITSSVTWLGFHFSLRPADACHPYGHGKIESLAGVFSGFSLLAVALLIAFQAVRE
                     LLTPHASPAWYTLPVLLMVVLVKEAVSRAVLRAGRATDSLALEGDAWHHRSDALTSAA
                     AAVGISLALLGGPRFAQADDWAALLACGIIVFNAVRILRKAFHEMLDGDVGDEMRFRV
                     MGEARGVPQVDSVSACRIRKSGTDYFVEVHIQVPGDLSVREGHAVGHQVRDAIMAAKS
                     GVRDVVVHVEPVETDREGDGAVP"
     gene            complement(17645..>18939)
                     /locus_tag="PK576_04420"
     CDS             complement(17645..>18939)
                     /locus_tag="PK576_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOE60868.1"
                     /translation="WRTVPTPEAVAALTPMLDDPKLKTEALKSLKVIALHTPVGVPDK
                     APGSVLTPLTFVPRRIGTYRSEACCVADFNGDGKLDIAAGPYLYLAPDWKAVRIRTVS
                     SNVGEDGKGYADDFCNLVVDVNKDGKPDILAACWFSKTSYWYENTLGSSKELWPEHVI
                     DPLGNHETGTLVDIDGDGKALEFLPDTGVTVWYEPGTLTNGQPGYVRHTISMDKTTFG
                     TGVGDINGDGRPDVIRPNVWYEAPKDIRKDTWKKHPIALGGKDGKVEHTSNIIVFDVN
                     KDGLNDLITSAAHKHGIFWYEQQRDASGTISWRQHLIDDTWSQPHYLAFADVDNDGNK
                     EVIAGKRFMAHNGGDPDAFGKLCVFYYRFTPGPNPVFRKHVVTYDEGVSAGLNIVPVD
                     IDGDGDIDLITTGKWGGPVIYENRMTEWVSDELEVYAQ"
BASE COUNT         3928 a         6149 c         5463 g         3399 t
ORIGIN      
        1 caatcgatcc cgatcccgat agcgatcccg accccgattt ggatgaaacc ctgaagcctt
       61 gaaaccctga aaccccgaaa ccctgaaacc ttgaaaccct gaaaccttga aaccaaagag
      121 agttgaaggt tcaactgctc tttttaggtt aaacgcgctg gccgctgtgc gccgcctcac
      181 ggagcggagg gcacgggcac gaacaggttc agcagcacac ccggacggat cccgtaaaag
      241 gacgcgcggc gcccggtgcc gaaacgtgcg tccgcaacat ccccctggtc tgccgccgct
      301 atcaccacca ccggatggaa cgactccggt atctgttcga tgcgcgtcca ataccccacg
      361 cgcgtatagg cgcggaggta ccaaggcaac ggccaagtgt cgggtacggg aaccgccacg
      421 gcaatcggcg tgtcggttcc ctgtgccgcc cgcgcggcct cctcaatcgc cgccaccaga
      481 ttcaacgcgt ccgtgcccgt atgggcgtag acatagggat tgcgcggatc cgcaggcaac
      541 cggaaacaag cccggtaggc ttgtgccgca tgtcgggcgg ccacgaacgc gaccagcgcc
      601 cacaccacaa cagtacccgc aaggcgaact ccccgtatgt tttgacgagc cagccacgcc
      661 accgcctgcc ccacgcccat gcccgccaac agaatcaccc cgtgcagaaa tgaaagcgcg
      721 caccaggggg tcttgtacgg aatcgcgctg tacagggccg tcaatacaag ggtgtagacc
      781 gcgaggaagc gtgtccagcg caaaaccctc gtctcctctt cactggcgtc cggaaagcga
      841 aggaaagccg ttaccgtcgc gatcagcgcg ggcaccaaca gggcggcctc gctccaaagc
      901 ggtccccgtc cgtatcggaa ccagaagaga atcttcagat agaaattcca gggatgttga
      961 tgttccggca cggcggtggc acgcttcaga tatgcctgcg ccgtggagaa gagcgcgtca
     1021 acgattccgc gcggataggt gaaaaaggat gaaaagagca gagccgccac cccgatcgcg
     1081 accccggcgg ccaagaccgc atcgcgtgtg cgccagacgc ggcgcaaccg gccaatcccg
     1141 caggccgcca cggtggccac tgccgccgcg gcaaacgaaa gcaccgccgt ctccttggtc
     1201 gccgccgcca gccccgcgaa ggcaccaaac cccgccgcag cccacagggt ccgacgcccg
     1261 gagcgccgtt cccccggcac ctcggcggca cgcaaccccg catagcgcac cgcgcacaac
     1321 aacatacccg cgagaaaggt gaccaacatc gtttcttgaa tgaaaaaccg gctgtagtag
     1381 accatcgcgg gtgagaccgc catgccgagc aacgcagtga aacgcgcact ccggttcaca
     1441 aacagaccct gtcgttccga cacccccatg gcaaacatga tcagcagcgt caacaggctg
     1501 aacgtcacgg gcacgagacg gtacgaccac tctgtggtgt ccgcaaaggt tcgtgcggtc
     1561 gcacggcaga agaaccaggc cgcgaagtag agcgacgggc cgtggtgatc cgtggggtca
     1621 tagtgataga ccccctcctc cagcaactga ccggttcgca ccgcctgatt cgcttcgtcc
     1681 ccatgcattg gacgttgtgc cagatggggc aaacgcagcg ccaaggcacc cgccagaact
     1741 ccgatgcaca acagtgcttc ccacgctttt cctaaccgca tcccggacac ctcaccgccc
     1801 ccggaccgga ccgaacggcg gcaatcccgc aggcccttgc tgttgcatca tctgctgcag
     1861 cagcggtccg gccacctgcc gcaggatcgt gttctcctga atgcgctgca cggcctgcat
     1921 cttccccaga ttgatcgcct tctgcagcgc gaactgcatc ggttgctgct gacgcttcat
     1981 ggcgtagagg gtcgccatgt ccagccacgc ctgccagtcg ttcggccgca actggagata
     2041 gcgtgagagc ggcgcggcca ttttatccgc gttccccgcc tccccgtaca cccggaccac
     2101 ctccagcaac tgctcgggcg gcatattctc cggcatgcgc gccatcacga tatccatggc
     2161 cttgcaagcg ctttcccccc gcttcagacc actgagcagc gaaccgatct caaacatccg
     2221 cgtcaccggc agattgggaa cagtcatgag ttgctccaaa tacttcgcgg ccgcatcgtt
     2281 ctgcccgagg tcccgatacg cgagcgccag ttcgaacact tcgtccggtt gcagggtttg
     2341 gctctgctgc atcttggtgg tcagggcgcg cactttcgcc tcggtgttca gaatccgccg
     2401 gatgaaatcc acaaacccgt acccgcgttc gttgttgggg tcacgccggt tgtactcttc
     2461 cagaatccgg atcgactcga cgtaccgctc ctggggtaac aggacttcct gcaccaagcg
     2521 gaaattggcc tcgggactga ccggatagag cgtgcgcgcc tcctgaaagg cccgctccgc
     2581 ctcggcgcgc aggccgtgcg cggcatacag gcccgcaatg gcgctacgca gtttggagaa
     2641 cgatttctgg ccgggcagat cgcgccggaa caatggatta cgcaacagcc tccgcacata
     2701 ccaatcccag aaatccagat cagcccgcac cgtctccgca ggaatggcct gacgctcagg
     2761 gttgatcttc aggatgagac cgtggggcgt catgaacgga aacatccagt tgatcacata
     2821 actctcctcc acgtaaaacg cgtggcgcgc tttgtttttc gcgaagatca tgtcgcacag
     2881 gatcccgttg atctccatca cgcccaaggc gccgctcacc tgcacccggc cgttttcaat
     2941 cgtcaaggcc gcattcttgg gacgcttacc ggcctgcacc tcatccacgt aaacctgaaa
     3001 ggcgcgcgcg ctgtcgtctg gcgtggggat ccagatttgg ttcccataga gatcgcgcat
     3061 ggtcgacatg taggtgttgt cagccaaggc gttctgcgtg atcaaaaaca cgtccggccg
     3121 tacctgggcc gagtaaatca tgtatgtcgg cacaaaccgg ccgggatccg taccgccgaa
     3181 aaagatcgcg ttgggctcca tcgcaggtgg aaacagcgga ttcggcagcg gctcctcgtc
     3241 cgcctccaac tcctcggtga tcgcctccgc accgcgcaac tggtagttgc cgaactgcca
     3301 accgaagtcg tgcccgttct gctccgcacc gctcatggag aaaaccagat acgcgttggt
     3361 gtagttctcg tagatcggaa tcgcggccac aagcacaccc gcacacacca gaccgatgcg
     3421 caacggcttc agcagacggg cattgagcca gccgagccgc accagccact tcagaatgcg
     3481 attgccgaag gtcagtccga acgccagacc gtagccgatc caaatcgcga acagcccgtg
     3541 cgaactgatg aacttgacct tctggataaa ggcgtcctgc aaatcgccct tgatgttcgc
     3601 cagcaccacc agaagcacac tcatgaccag aaaccccgct gcggtcgcga tcatccactg
     3661 ctgggtcacc tgatccatgt cggaacggaa atcaaaacgc ttttccgcaa aggaacggaa
     3721 gaaaaaccag cccgccccgc aggcggcaag gatcaccagc gccagcgcca gcgaagccag
     3781 ccggaccccg atgccgggat cgcccgtgcc ggaaagcgca tgggtcaggc ctttggcgag
     3841 aaaaagcgcg atcaccgcca gcatcccgca cagcgagatc cccacagtca gcccttcaga
     3901 gaggttgcgc gcctgccagg tgcgtttcac aaacgcttcc acctgcgtga gcaccacggt
     3961 cgcccccccc accaccagca gaaacaggat cagtccgatc agcagcttgt cgagacgcca
     4021 gagcggttca ccgccggcaa actcggagat cagcaccagc acgcaggtca acaggtacag
     4081 gccgctggcg acataccctg cgcggatgcg ccgtttcccg acccggaaac tccaaagcgt
     4141 aaagggcaaa aagcccagcg tggcggcgat cagcgtgaac tgcacacgca agtcggtgaa
     4201 gtaggtgccg agttggaaga ggaacttttt ggtgaacaca tcggacgggg caagtttttc
     4261 gtactgaccg cgggtgatgg cgtgtttgaa accttcccac gtacgcgggt atccccagtt
     4321 catcggcggg ttacgcagat ccgaaacgat cggcatgtag atgtagaagg agacccccaa
     4381 ctccgcgaac agcgtggtca cagccaccgc ccgcccgttg ggaagcagtt gccaggccag
     4441 cccaatcacc acgaaattcc agatcaccgg ccaccagaac caccaggagg tcggatgggt
     4501 cagtccgttc atgccgcctt tgcgcaacag cacaaacacg gccagttgca ccgccgccac
     4561 cggcagggcg tagaagatgc cgcgcggcag cgtgaccgcc agcagaaaca ggacaaccaa
     4621 tcctcctgcc agcaccagag tcggcaatga ccagttatac ggctgcccca tgtatccggc
     4681 gggcatcgcc gcgccccgga tggcagagag ccggaaaaac aacaacaaaa gcgatacgcc
     4741 tgcggcgatc agccacggca gggtttcccg ctcgccccac cggcttttaa agacatacgc
     4801 gccgcctaaa ctgaaggcca gcaccagtac ggccaacgca gccacgctgg tgtacacact
     4861 gggatcgaac agcggcgcat cggttgccgc gacctccttc atccgggtcg tcgcggtgga
     4921 aaagaaaatc agcagcgcac ccagaatcgt cacgcccgcc gtgatccatg cgccttgaag
     4981 agccagcgca tccagccgcc cccccttgcc ggttccgggc tcgcgcttgc tcaccgcgtt
     5041 tcggcgccaa gataacccgt ggcccaccgc caaccccagc aacacacaga cgcaacccgc
     5101 aaccagcaga cccggagacg gcacggaaac tttcgaaagc ggctccgctt tagcaaacgc
     5161 ggggccgccc ataccgggcg aggccggcac catcgctccg atctgcaaca gatgggaggt
     5221 caacagcatg gggatgccca ccagaagaaa atcgcggaac agtgcgatgt cacgcaggaa
     5281 aatcatcacc accagcggca gggccgccag cagcagcacc tgataattcg tcagccccaa
     5341 gccgaacacg aaagccgtca gccacaaggt tttttccgcc gggcaccgca tccagcgata
     5401 ggtcagcaga aagatccaca tgaggaaaaa agcgttgagg gtgtacacct ccacgatcgt
     5461 ggcctgcgac cacatcaccg ggctgaacgc gaaaaccaga ctgccgccca caccgccggc
     5521 ccagcagagc aggtcactct cctgctgtct gaaatcctcc tcgccctcgt gcgcgtcccg
     5581 catcatgtcg gaagccgaac gcgtgatcag cattgccgtg atgccggccg ccaacgcggc
     5641 gaacaccgca gacatgagcg aaatgctcca ggccggtgtc ggctgtcccc ggaacgtgac
     5701 ccaactgaaa acccgggcga acagataggc gcacatcgtc cagatgggat aaccgggcgg
     5761 atgcggcaca cccagatgat ccccggccac cgccaactcg cccgaatcct ccagtgtgac
     5821 ggagggtccc agcgtaaaaa agtagacgag gaaagagatt atcgtggcgg tccagaaagc
     5881 ggtccagtcg atccgccgga aaaaagcaag attggggcca gagcccgaaa accggttttt
     5941 catcataacg acaactcacg cttgtaaaga agacacttaa tttacaatcc ttgcctctca
     6001 ccggcaagga caactttccg tttatacgcg ccacgaggag ggaacacggc ttgaacaacc
     6061 ggggcggctg tgttacgttg ggcacatccg ttcccggacc ggacagcccc ggcgggacag
     6121 agggagcgct catgccgata caggtgatca ttgtagaaga cgacccccgt ctgcgttccg
     6181 gattgcgcga aatcatcaac agcgatccgg cctgcgcctg cgtcggcgcg tacggttcgg
     6241 gcgaggaggc attgcaacag gcgcctgccc tcaacccgca ggtcgcggtg atggacatca
     6301 acctgcccgg catggacggc atcgcgtgcg tgcggcatct cgcggaaatc ctgcccgaac
     6361 tccagatcgt gatgctgacc gtttatcagg acaccgacaa cctgttccgc gctctggctg
     6421 cgggtgcgca cggctatctc gtgaaaccgg cccgcgcccg ggatctgctg gacgcgatcc
     6481 gcgatatcca ccgtggtgga gcccccatga ccagcgcact ggcgcgtaaa gtcgtcgacg
     6541 cgttccgtga ccgcgcctcg cccgcgcaac cgccgcccgc cgccaccccc ccgaccaccg
     6601 aatccgatga gttggcgccg cgggagaaag aagtgctcga tctgctggcg cagggtttct
     6661 cgtacaagga gatcgccgat aaactgaatg tcgccttcgc gacggtgcac acctatgtcc
     6721 tgcggatcta caagaagttg catgtgcgat ccaaaaacgc cgcagtggcc cgttggctcg
     6781 gttatcaacc tggcgaatag gagaaaacgc caaccatgac cactccccga ccgttcggac
     6841 gtcgcgcgtt tttacggctc gcccatgcag gcatggccgt tctgccggtc atgacggcaa
     6901 agcgagtctc cggacagtcc gctgcggtgc agccagcaac gcgcacctat gcgcgccaag
     6961 tgaaactggg gctggtcggc tgcggcgggc gtggttcgtg gatcagcaat ctattcaagg
     7021 cgaacggcgg gtttgtgatg accgctgtgg ccgattattt cgcggatcgc gcaatgcgcg
     7081 tcggcgccca actgggcgta ccgccgtcac gctgtttttc aggactggat ggttaccgcc
     7141 gcctgatggc gtccggcatc gaagccgtag tgctgcagac gcctccttat ttcttcccgg
     7201 cacatgcgga agcggccctt gatgcgggat tgcacctcta catggccaaa cccgttgcga
     7261 tcgacatccc cggcaccctg acgatccaac ggcttgcggc ccgggcgaca caggagcggc
     7321 gcgtgtttct cgcggattac cagttgcccc gcgacccggt gaatcaggag gtcgccaaac
     7381 ggatccgggc cggtgcgctc ggccgactgc aaaccgtttt ctcctccggc tgggcgggcg
     7441 gcaacgggta tcaggacccg cctctggaga ccccgatcgc gaaccggctc aaagacctcg
     7501 tttggtgcaa cgatctcgcg ctcggcgggg attatctggt gcattacgac gtacacatcc
     7561 tcgatgcggt ggtctgggcc ttgggaaaga aaccggtcgc cgccatgggc gcatcggcag
     7621 cgctcaggcc ctccgtgcac ggcgacgcac gcgactccag caccgtcgtg tttctgtttg
     7681 acgacggcac gatctggtgc catcagggta tgctcggatc gtatcatgac tggctcaagg
     7741 tcggccggct caccgcgtcg ctacagggca cgcagggcgc ggcttgtctc tcctacgcag
     7801 gaaaggcata cgtgcgtggc ggaccgcgcc atttcagcgg cggcatcccc aaccccgagg
     7861 caagcgtcaa aaccaacatc gccgaattcc ggcaggcgat cgagacctct gcctgcgaca
     7921 acgcgcaggt tctgcgcgcg gtcgacagca acctgacagc gatcctcggg cgcgaggccg
     7981 ccgcgcgccg ggatcgggtc acacttgaca cgctgatgcg cgaagcgcgc gttttacaac
     8041 ccgatctctc cggcctgata cgctgaccgc gccgtcggcg cggcccctta ccacggccac
     8101 catcctgtcg gggcggagac gcccaaccga aggtacggca acaccgcctc ggcgtgcgtg
     8161 tcgtagttga acacagtgaa gccggacaag cccaatttgc gggtgatgag gcgcgcgttg
     8221 ccgacccact cccgctgcaa ggcaacggaa ctgcggaaag cgacgttagc ctccgtgaaa
     8281 tcaagtcgcg cacattgatc tcaatcatgc ccgcttccgg tcctgtgggc ggcgttcacg
     8341 ccgccaaacg cggacaggcg gccgtgcgaa ccccgcctct tcccacgagg cctgcggcgt
     8401 cccgctcatg ggcacgcaca gcgcgcgcaa cgtcttggtg tcgcgcaccg cttgccaagc
     8461 gggaaacacc gcctccccct ttggcggcgc agggatcgtc tcagcccttg ccccgccgat
     8521 ccccgccaca agcagaaaca cgatccgaac gatcttcatc ccttgcctcc gatccagtaa
     8581 acacataata ttttaccttc aatggatcga aaagccaaga atgcaccaca aggcgggatc
     8641 atccgcatgt tccgcaagga caaaggcctg tctcggggaa acgcaccgtg acaatggaac
     8701 aggacatcac ccgattcaac ctcgtctcgg catatcagcc tgcaggtgac caacccgagg
     8761 cgatcgccgc cctttcgcaa gggttgcgcg cgggtgcgcg tcggctgacg ctggaaggag
     8821 tgaccggatc cggcaaaacc ttcacgatgg ccaatctcat ccgcgaatgg ggtcggccga
     8881 ccctggtcct ctcacacaac aagacgctgg ctgcccaact gttcgccgaa ctcaaagggt
     8941 tctttccgga tcacgccgtt gagtatttcg tcagttatta tgattattac caacccgaag
     9001 cctacatccc gcagaccgac acgtatatcg aaaaagacgc ctcgatcaac gaggagatcg
     9061 aacggtaccg gctcggcgcg accaacgccc tgctcggacg gcgcgatgtt gtgattgtag
     9121 cctcggtctc ctgcatttac gggctgggat caccggagga ttatcgtgag atgctggtcg
     9181 acctcacgtc cggctgttcc gtcgcgcgcg atgccgtgct ggagcagctc gtcggcatcc
     9241 aatacgttcg taacgattac gagccgcagc ccggcacctt ccgggtgcgc ggcgacagcg
     9301 tcgacatctt tccctcttat gagaccgacg ggttacgcgt cacgttcttc ggtgacgaag
     9361 tggagtcgct gcatacgctg gacccggtga ccggccggac cgggagtccg ctttcgcgcg
     9421 tggtgatctc ccccgccaaa cacttcgtca tgccgcgggc gaaaattgac gccgcaacca
     9481 gccggatcgc gtgcgaactg gatgcgcggc tggccgaact ggagcgtcag gggaaactgc
     9541 tcgaagccca acggctgcgg caacgcacgg actttgactt ggaaatgctg cgcgaattgg
     9601 gatactgcaa cggtatcgag aactactccc ggcatctggc cggacgtgtc gcaggcgagc
     9661 cgcctgcctg tctgctggat tatttcgagg gggagtttct caccattctg gacgagtcgc
     9721 atgtcacggt gccccaattg cgtgccatgt acaacggcga ccaggcacgg aaacagactc
     9781 tggtcgagca tgggttccgt ctgccctccg cccaagataa ccggccgctg cgctttgagg
     9841 agtttctgga caaggtcgga ccgatcgtgt tcgcttccgc cacgcccgga ccgtacgaac
     9901 gtcaggttag cgaaatcacg gtcgaacagg tgatccgccc cacggggctg ctcgaccctc
     9961 cggttgaaat ccggccgctc gacaatcaga tcgacaatct gatcgaagag atccggcgcg
    10021 ccgcggaaca gggcgaccgc gttctggtaa ccacgctgac caagcgttcc gccgaagacc
    10081 tcaccgcata cctcacggag atcggcatcc gggtggaata tctgcacagc gacatcggcg
    10141 cgattgatcg cgtcgccatt ctcggccggt tacgcaccgg cgcgttcgac tgccttatcg
    10201 gcatcaatct tctgcgtgaa ggtctcgatc tgcccgaagt cgcgcttgtg gcggtgctcg
    10261 acgccgacaa ggaggggttt ctgcgttccg ccacctcgct gatccagacc gcaggccgca
    10321 cggcacggca cgtgaacggc cgcgtgatcc tctatgcgga tcgcgagacg gaggcgatac
    10381 gcaagatggt cgacatcacc cgcacgcggc gccagcggca ggaagccttc aatgcgacgc
    10441 acggcatcac cccgcagagt gtccgaaagg cgctcaacga atcactggat ctgtatgctc
    10501 aggcccggca catcgaggag cggatgttcg ccgcagagtc gtccgaatcc tacgacctgt
    10561 gccgggcaat tgccgacacg gaggcggaga tgatcgctgc ggccgatgcc ttggaattcg
    10621 agcgcgcggc gctgctgcgc gatgagttgc gcgaacttaa acacctgctg gccacacccg
    10681 ccgggccgga aaccgacggt gccaagtcca cgaaaccgaa gaaccgcaga acgcgccgtc
    10741 cgcagggcaa aggcaaagcg gccgcgccgc cgcaaccggc tggaaaacgc tcggtgcgcc
    10801 gccgcccgta gtgtgcggat ggcacacgct agagccgctg gacgaatgcc gccatgcggt
    10861 ccatcgcctc tttgagctgc tccatgcccg tcgcgtaggc gcaccgcagg aaacccgcgc
    10921 cgcaggcacc gaatgccgtg cccggtatac aggccacatt acattcgttc agcagacgga
    10981 gggcgaaggt ggtcgaatcc aacccggtcg actggatgga gggaaaaacg taaaacgctc
    11041 cgcgcggcat gaaacaatcg agccccatct cattgagcga ggcgacgata taattccggc
    11101 gcagatgata cgattcgcgc atcgcggcca catccgccgc gccatgctga agcgcctcga
    11161 tgcccgccat ctgggccgtg gacgaagcgc acatcatgca gtactggtga atcttcatca
    11221 tggcatccgt cagcggcgcc ggtgcgcaga catatcccaa acgaaaccct gtcatagacc
    11281 aagccttgga aaacccgttc aggaaaatca gtcggtcgcg caagcgcggg atggccgcta
    11341 aggaatggcg tccgccctcg tacgtgagtt ctgaatagac ctcatccgaa accagcaaca
    11401 ggtcatggcg gatgacgaaa tccgccagag cctcggtatc ctcgcgccgt agcgtggcgc
    11461 cggtcgggtt gttgggaaaa ctgacgagca ggacccgcgt acggggcgtc accttctttt
    11521 ccagttcctc cacggtgagc cggaactcat cgcggcggaa ggtttcgacc gcgaccggca
    11581 caccctgagc catgcggatc aggggcgcgt aggccacaaa gcaaggctcg tggtacagca
    11641 cctcatctcc gggcgataac acggcgcgca tcgcgatgtc gaatccctcg ctgaccccaa
    11701 cggtgaccaa aatctctgtc tgccaatcat attccgcccg gaaggtgtca cgcatgtatc
    11761 cggccaactc ctgacgcaac tcgggcatgc cgaggttcga ggtgtaccgt gtcatgcccc
    11821 gttcgagcga ggtgatcgcc ttctcgcgga tatgccaagg cgtctggaag tccggttcgc
    11881 cgattccgag cgagatcaca tctttgcggc tcgccacgat atcgaaaaag tcgcggatcc
    11941 ccgacttcgg caggtcacgc accagcgggg agacgaaatc atgggcagac ttggagacgg
    12001 tcgtcatggc ttacgccttc catcaggaca ccctgatcct tgtatgtttt caatatgaag
    12061 tgtgtggtag tcgaaagcac accgtcgagc gaggcaaggt gtgcactgac gaactgggcg
    12121 acctgctgca ggctctgtcc tttgacgaac aacagtaaat cgtaggcgcc cgacatcaga
    12181 cacatggact ccacctgcgg gaacttgccg atccggttgg cgatccggtc aaacccgccc
    12241 tcgcgttccg ggcggatgcg caactcgatg actgcccgca catctgtttg ctcaatgcgg
    12301 tcctgattca acacagcctg gtacccccgg atcaccccgt cgcgctcgta tgcggcgatc
    12361 cgttcgcgca cttccgtttc ggtcagcgcc aactttttgg ccagcgtcgc gtccgattca
    12421 cgcgcgttct ttttcagcag ttcaagcaat gtatccataa tactcgcctt tcgtgagggt
    12481 gcagtatagc gaaaaccgtt cggactgcaa tgtcagagcg gctcaatctg ttcacaatcg
    12541 gtcgtgacgg tcaccgcctg gccaatcagt tcaatgttga gtacgacccg tacggttccg
    12601 gccagacgct tcacgcgacc ctcgatcccc atgaacggac cgctgagaat tctcaccatg
    12661 cggccctctg tcagcggcgg gccggccttg agcgcggggt tggcccgtaa cgcccgccgc
    12721 accatgatca gctcgcgcag cagacgccgc gcgtttatgg gcaccagaat ccggaccagc
    12781 aggttggtct gcagcaacgc taagcgctgg gcatccggga aacaggcaaa cacatacccg
    12841 gggaaaatcg gcacccggac ctggattttc cggcgctgga caatccgttg ccgttcgcgc
    12901 aacggcaggt aaaacgggat accgtaaagc cgacagtact ccgtgacctt cttctccgtc
    12961 cgcggcttga caaacagcac tttccaattc ttcatgatcc cgccgcgtca gccctgcgtc
    13021 tcctccgtat cggcatcggt gtcggtatcg atatcggcat cggtgtcggc atcagcgata
    13081 ctgacggaca cgagcgtatc cccgtcatcc aagcggacca gccggacccc ctgggccgtc
    13141 cgcccgcaga gacgcacatc gtttaccggc gagcggatca tcatgcccgc cgaagtgatg
    13201 gaaataaagg actgctcctc ccgaacggcg tgtgcggcga ccaccttgcc gttacgtccg
    13261 gccacatcct tgatggcgat catgcccttg ccgccgcgat tatgcggcgt gaactcggag
    13321 aacatcgtac gtttaccata cccgttttcg gtggcgatca acagcgtggc ctgatcatcc
    13381 accacgtcga aactacggac cgcgtctcct gcccccagcc ggatcccgcg cacaccggtg
    13441 gcggcccgcc ccatgcgtcg cacctgcttc tcgttaaacc gcatggcctg cccgttggcg
    13501 gtgatcagca gcacatcgtc cccggaacgg gtcagccgta cctgaatcaa ccggtcatgt
    13561 tccccaatgt tcaacgcccg gatgccgttg cggttgatgt tgcggtaatc gacgagcgcg
    13621 gtctttttga cggtgccgcc ttcggtggcg aacatcacgt ccacgtcctc cgcaaatgcc
    13681 cggatcggca gcagggccgc gacctgttcg ccttcctgaa actgcaacag attcacgatc
    13741 ggtttgccga acgaggtgcg gggcgcctcg gagatttcga aggcgcgctc cgcgaacacg
    13801 cggccgaagt tggtgaaaaa gagcagggtg tcatgggtct cggcgacaaa gaccgtggtg
    13861 atcaggtcct cctccttgat cgcgacaccg gtgatcccgc gtccgccgcg cctctgctcc
    13921 cggtaaaccg tcaacggtac gcgtttcaca tatccgcgca ggctgagggt gatgacgcac
    13981 ggctcgtccg caatgaggtc ctcgatgttt acctcaccct ccagaggcac gatctccgtg
    14041 cgccgtttgt cgttccgccg agcgtggagc acatactgcg ccttgagttc cgccagatcc
    14101 tgtttgatca gtccgtagat tttagcgggg tcccgcaaga gttcctgcaa atacgcgatc
    14161 cggtcgcata actcgcggga ctcagcctcg accttatcac gctccaaacc ggtcaactgg
    14221 tacagacgca tctccagaat cgcatccacc tgccggtccg aaaaggcgaa acgctccacc
    14281 agccggactt tcgcctcttc gcggttggaa gacccgcgaa tcagggcgac cacctcatcc
    14341 agatggtcca gcgcgaccaa gagtccgtcc agaatatgtt tgcgcgcctc ggctttcgcc
    14401 agatcgaact gtgtgcggcg ggtgatgacc tcgaaccggt gatccgtata actccggaac
    14461 agatccttga gtgtcatcac cttgggccgc ccctgatcga tcgccagcat gttggcgccg
    14521 aacgtggtct gaagctgtgt gtgtttgtac aggttgttca gcaccacctg cgccatcgcg
    14581 ccgcgtttga gttccaccac cacccggatg ccgtcgctgg aactctcgtc gcgaatgtcg
    14641 gagataccct ccagcacttt gccgttcacg agatcggcca tcttttcgat catgctggct
    14701 ttgttcaccg tgtacgggat ctcggtgatg aggatcgcct ctttattctt gtactccacg
    14761 atctccgctt tgccccggat agtcaacgat ccacgcccga gtttgtacat ggcctcgatg
    14821 ccgcgtgtcc cgcacagtat ggcaccggtg gggaaatccg gccccttgac aaattgcatc
    14881 agctcgtcaa tcgtgcactc cggattctcg atgtagtggg tgagcccgtc cacgagttcg
    14941 ccgaggttat gcggcgggat gttggtggcc atgccgaccg caataccggt cgacccgttc
    15001 aagagcagat tcggcagccg ggcgggcagg acagtcggtt cctgaaactc ctcattgtag
    15061 ttgggtttga aatccaccgt ctctttttcc agatcatcca gcatctcctc cgccagacga
    15121 tccatgcgca cctccgtgta ccgcatggct gccgccggat ccccgtcgat ggaaccgaaa
    15181 ttcccctgcc cgtccaccag cggatagcgc atcgagaatt cctgcgccat gcgcacgatc
    15241 gtgtcgtaca ccgcctgatc gccgtgggga tggtatttac cgatgacatc gcccaccaca
    15301 cgtgcggatt tcttgtatgg cttgttcgat gtgttcccca gttcgttcat cgcgtacaat
    15361 acccgacggt gcaccggctt cagcccgtca cgcgcgtccg gcaaagcgcg cccgatgatt
    15421 acgctcatgg aatagtcgat atacgaggaa ctcatctcgt cttcgatatt gatgttttgc
    15481 aacccttggt cggtctgctg gtccggctga atcccgtcgc tcatgatgac tccgcttttg
    15541 aacagtgaaa gtggggctca gtataccaca tcagccgaaa aaaaaacacc ttttcccgaa
    15601 tcggatcctg tggtatttta cggtatgtga gaagcgattg aaccctttgt tgaggagcaa
    15661 accgaaatgg ataaccggac cccctgcccg atctgccgca ccccgaacat ttcgtatgcc
    15721 agtgccggag ccaacaccag tgaaatcaac tgcccgcgtt gcggccgcta tcagatccag
    15781 gcgttcgctg aaacccaact ccacaacagc ccgcccgctt ttcccccggc ccatctgctc
    15841 tccggattgt gccgcaacac ctgggaccat ctgggggaga aagtgttgat caccgtggat
    15901 ttgttcaagt cctgggccga tctggaacgg gtcgccaaac tccccgtacc gcgcgacacc
    15961 gatgtggtga ccaaggggga atacttgctg aagcacctgc gccgccgatc gaaaacactg
    16021 gcggacaggg tttcgtttac gccccatgaa ctggcgtgcg ggttctgctc cacgaaaaac
    16081 gaactgcttt tctgcctgcg ttatctggca gaccgggggt gggtggagga atgttcagcc
    16141 gccaatcgca agggacctcc tcgtgacgcg atcacgtttc gcctgacgcc cgccggatgg
    16201 gcggcattgg ccgctccggc cactccggcc caaccacgtg ccgtggtgtc ggtcatgctg
    16261 gatcgcgaag acgagtctct ctggacgcaa gggttcgccg ccgcggcgca ggccgccgga
    16321 tatgtggcgg agcgggttga gaaccgtgaa cagctcaaca aaattaatga cgagttgatc
    16381 gtatcgctgc gccgcgccgc tttcgtgatc gcggatctga ccggacaggc ccccatgacc
    16441 tatttcgttg cggggttcgc cggtggactc ggtaagcccg tcttctggac ttgcgaggaa
    16501 agcgaagccc gcgacaaacg gttgtggctg gaaacacgcc agtcaatcat caccacctgg
    16561 acgcgggaca agccggaggt gttcgccgcg cgtctcgcgc aacgtatcga agccaccatc
    16621 cccggagaat gagcgcatgc gcaaacggac ttagggaacg gctccgtccc cttcgcgatc
    16681 cgtttcgacg ggctcgacat gcaccacgac atcccgcacc cccgacttgg ccgccatgat
    16741 cgcgtcccgt acctgatgcc cgaccgcatg gccctcgcgc accgataggt cgccgggaac
    16801 ttgaatatgc acctccacga agtaatcggt cccgctctta cggatccggc acgcgctcac
    16861 cgagtccacc tgaggcacgc cgcgggcctc ccccatcacc cggaatcgca tctcatcccc
    16921 cacatccccg tcgagcatct catggaacgc cttccgtaga atgcgcacgg cgttgaatac
    16981 gatgatcccg cacgccagca gcgcggccca atcatccgcc tgggcaaaac gggggccacc
    17041 cagcagcgcg aggctgatcc ccacggcagc cgccgcagag gtcagggcgt ccgaacggtg
    17101 atgccaagcg tcgccctcca gtgcgagact gtccgtcgca cgaccggccc gtagcacggc
    17161 ccgcgagacg gcttctttga ccaacacaac cattagcagt accggcaacg tgtaccacgc
    17221 aggcgaagcg tgcggcgtca gcagttcgcg cactgcctga aaagcgatca gcagggccac
    17281 tgccagtaag gaaaagccgc tgaacacgcc ggccagagat tcgattttcc cgtgcccgta
    17341 cgggtgacag gcatcggccg gccggaggga aaaatgaaac ccgagccaag tgacggatga
    17401 tgtgatgatg tcccctaagg attccacccc gtcagcgatc agcgcatagc agttccccat
    17461 cagccccacc gttatcttga ccagcatcag gctgatgttg acgcccagcg tcaaaaaacc
    17521 gagccaccga atggatttcg attgcgacat atcggaacga tcacataaaa gccccatacc
    17581 cgtcaatcca atccgatcgt aaagaataac gcaggggaaa aagtgctaca cgcaccttct
    17641 cttctcactg cgcgtagact tcgagttcat cggaaaccca ctcggtcatc cggttctcat
    17701 agatcacggg accgccccat ttgccggtgg tgatgaggtc gatgtcacca tcaccatcaa
    17761 tgtcaaccgg cacgatgttg aggccggcgc tgacaccctc gtcgtaggtg accacatgct
    17821 tgcggaagac cggattcggg ccgggggtga aacggtagta gaacacgcac agcttgccga
    17881 aggcatcggg gtcgccgccg ttgtgggcca tgaaacgctt gccggcgatg acttccttgt
    17941 tgccgtcgtt atcgacgtcg gcgaatgcaa gatagtgcgg ttgcgaccag gtgtcgtcga
    18001 tcagatgctg acgccaagag atggtgccgg atgcatcgcg ctgctgttcg taccagaaga
    18061 tgccgtgctt gtgggccgcg ctggtgatga gatcgttcag gccgtctttg ttgacatcga
    18121 aaacgatgat gttggaggtg tgctcgacct tgccgtcttt gccgcccaag gcgatggggt
    18181 gctttttcca tgtgtcctta cggatgtctt tcggggcctc gtaccaaacg ttgggccgga
    18241 tcacgtcagg gcgtccgtcg ccgttgatgt cgccaacgcc ggtgccgaat gtcgtcttgt
    18301 ccatggagat cgtgtggcgc acgtagccgg gctggccgtt ggtcagcgtg cccggttcgt
    18361 accagacggt cacacccgta tcaggcagaa actccagcgc cttgccgtcg ccgtcgatat
    18421 cgaccagcgt gccggtttcg tggttgccca gcgggtcaat gacatgctcg ggccacagct
    18481 ctttggaact gcccagtgtg ttttcgtacc agtaggaggt tttcgaaaac cagcaggccg
    18541 ccagaatgtc gggcttgccg tccttgttga catcgacgac cagattgcag aagtcatcgg
    18601 cgtagccttt gccatcctct ccgacgttgc tggaaacggt gcggatgcgc acggccttcc
    18661 agtcgggggc gaggtagagg taggggccgg cggcgatgtc gagtttcccg tcgccgttga
    18721 aatcggccac gcagcaggct tccgagcggt aggtgccgat gcggcgcggc acgaaggtca
    18781 ggggcgtgag cacggagccg ggcgctttgt ccggcacacc caccggggtg tgcagtgcga
    18841 tgactttgag ggacttgagc gcctcggttt taagtttggg atcatccagc atcggtgtga
    18901 gcgcggcgac cgcctcgggg gtgggcacgg tgcgccaag
//
LOCUS       DAOSLE010000041        18877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3155_length_18877_cov_7.615450, whole genome shotgun sequence.
ACCESSION   DAOSLE010000041 DAOSLE010000000
VERSION     DAOSLE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18877
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_18877_cov_7.615450"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PK576_04425"
     CDS             <1..380
                     /locus_tag="PK576_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60869.1"
                     /translation="LPIAVRTNIVLNVRGIIRGRDRLNDTVRHAVRTPIRLWKTAEMI
                     VLGHILFGLGSLACFIGEIKMLTLAYRRGPGWFLSCIFLAPICWLALLAVDCKLAIKP
                     FVLACAGLIAIGIGGSLAGIEFG"
     gene            545..1045
                     /locus_tag="PK576_04430"
     CDS             545..1045
                     /locus_tag="PK576_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOE60870.1"
                     /translation="MKKLAIVILGAPNDAEGNLSSIAIERCQRALTEYAANPDAKMLP
                     TGGWGDHFNTTDKSHGYYTRQYLMAHGVPERNILECAESANTIQDAQLVRPIVRRHEI
                     DALIVVTSDFHIPRARFLFEREFPDLRLSFSAAQTHLPEEELGRLKEHEAKALARLKQ
                     SNAIGN"
     gene            1232..2197
                     /locus_tag="PK576_04435"
     CDS             1232..2197
                     /locus_tag="PK576_04435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE60871.1"
                     /translation="MKNCTLARLDEDEKARQSVLPHCRLAPGEIWIDPVRGHRIGCVD
                     ATDARQIAELMDGQPASLAVHDPPYNLVAFGKRSVGDFISWCARWIETTSASLCADAS
                     LYVWLGADQNEGFQPLPDFMLMMRGFKEFSSRSLITMRNQRGYGTQKNWMAVRQECLY
                     YTKGSPSFAVQYTDIPKILRGYYKEINGKKTENMERSKSENIRPGNVWVDIQQVFYRM
                     EENVNGCYAQKPIKAIERLVAASSREGDTIIDFFGHSGTTLIVAEQMKRRCMTCDVDP
                     IYCEIMIRRLERLRTYGKTGWQNGNPFEKELHGVVESEQPVLELW"
     gene            2197..2913
                     /locus_tag="PK576_04440"
     CDS             2197..2913
                     /locus_tag="PK576_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60872.1"
                     /translation="MERLKTQLETLILELEDSTEIYERLENLISVYPFNEYEYMVSTL
                     MGRGKLSLDDYYALRDEYIGRNMYLYIFEISAPRGFGETWAQGHLKELVPELQKPSKK
                     KDPAYSGQYDFCLDDRIAIEVKASRAVDFELQAPLYVKALASDSTRPFDMNFQQVKPG
                     CCDVFVWVGVWRDKIRYWVLASREMESNRYYSKGQHRGNVGEGQLHLRHDNIAEFADY
                     EVIGRDLLQAIKAAYDRQHA"
     gene            3113..4375
                     /locus_tag="PK576_04445"
     CDS             3113..4375
                     /locus_tag="PK576_04445"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009090120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOE60873.1"
                     /translation="MSKKNSGTAYRIACERYAEMGVNVEAALGKLAQIPISLHCWQGD
                     DVRGFENPEGDLTGGIQTTGNYPGRARTPQELRGDIAFVLSLVGGSHRVNVHACYAEF
                     EKGKKVDRDALQPEHFKNWIAWAKQNRLGMDFNPTLFSHPRFDGFSLASRDKRTRNFW
                     IRHTQATRRIAAAIGKALGTPSVNNVWIPDGYKDIPADRATPRKILNDALDECFAEKL
                     NPRHMLDAVESKLFGIGAESYTVGSHEFYMGYAVKNQKLLTLDAGHFHPTETISDKLS
                     ACLPFVPGILLHVSRGVRWDSDHVVLVGDDLIAIAQELVRLDQFRRIHIGLDYFDASI
                     NRIAAWVLGMRAMQKALLYALLEPFETMREAEREGDLTARLVWTEAAKTMPFGAVWEK
                     FCLEEDVPPDPAWLNEVKRYEAQVLAKR"
     gene            4372..5469
                     /locus_tag="PK576_04450"
     CDS             4372..5469
                     /locus_tag="PK576_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOE60874.1"
                     /translation="MSGGQCVGWESRVLLEAVTELSHAFGSAAYVKGGGGNTSCKQAD
                     TLWVKPSGTTLAALTPDRFVALDRARLACLYRETPPDGAAAREAWVKERMDAAVRPET
                     PGRASVEAPLHDTLAARFVVHTHPEACNGMTCAVGGEAAAARLFPEALWIPYVDPGYT
                     LCREVRCAVQDYAREHGAEPALIFLQNHGVVVSGDTVEAVRAVYESLMERLSREYAEA
                     HLDGGAHVGPEPAEHEVAGWRQTLSETFGSEAAAVAVSGMLDVGAGPISPDHVVYAKS
                     YPFEGRVTPENLRAFQSVRGYPPRVVVTGSAVLGVGPRESVARVALDLARDGAEVKRL
                     ARAFGGLRYLSDAARQFIENWEVESYRAQQM"
     gene            5559..7292
                     /locus_tag="PK576_04455"
     CDS             5559..7292
                     /locus_tag="PK576_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE60875.1"
                     /translation="MRRAGSYLRKVRVGHGLTVFGVAGVCLLAPIACMTPGRAVLETD
                     ETGTRLATAYWQQQTGSTNTFDMSRPADTLTLRIALLAAARGEQNVIFPAIPNLGELN
                     VSNEVIVLTLPHALCLAARNDRSYQKLKEAVFDAALDLDYQQYRFGTTFSGMMFGLLS
                     GDPGIEKASGGVSAGAARQTEAGLAVAGRLALNVVSLLRDDWRSMGLTGDLSMTLPLL
                     RGAGREIVREPLTQAERNLTVAIWNFERYRQTYAYSVASAFYNVLEYGQRLKNAIDNE
                     SRLQENSRRAEMWFEAGRMERIQVDQARSDLLNAGESVINTRKNVEEKLDAFKIQIGL
                     PPEAKIVLDERELDELEKEMERIAERSPNAVEAFPEEDEACRIALAERHDLLITRSAL
                     EDVARSVKIAADALRADVTLQGGGSLDRLRETGRSGFKGGEALEGGLSVSLPWDRRRE
                     RNAFKKRLLELEQAKRALEAMEDTVKQAVRSGFRNLIAARASYENQQETMRVARLRLE
                     SNNLFLLSGRSSMRDILEAESAMLTARNALCSAVIQWRLSELALRRDMGVFGIGEDGM
                     WRETVDRGNHG"
     gene            7285..8895
                     /locus_tag="PK576_04460"
     CDS             7285..8895
                     /locus_tag="PK576_04460"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOE60876.1"
                     /translation="MVKKTSKKKRIAVGTLLVLGAAAWFWFGKRDKTSEAVPVFAVQQ
                     GPLTIAVTSGGSIQSRDKIVIASELEGNNTVIWVIDEGVSVQPGDLLLEFDSSDLIEK
                     RNEQEIVVANAEANLIITQEKLGITEGDCEAALLDREVDMMLAKMALEKYQDGDYPQQ
                     LRQSEADVALADEELKRADERLEWSQRLAKANFLTRTELQADQLALQRKKLDLAMAET
                     KMSVLTNFTVLQQRATLESALRKATRALKRTTWQNKATLRQVETELQARQRELNRATN
                     RMAELNFQIEKSKIYAPTNGIVLYTSTVLISRRQWWIKPLAVGSTAVERQELIYIPLE
                     SGMVVEVMVPEASLNKIENGMEARVKVDAFPGRVFEGHLVKIGILPDGQSSQLNPDLK
                     LYKCEIECDFKEVNIRPGMSCDVELVKETYENALYVPVQCVVRVEGVPRVYVRDDGEW
                     VPRIVSVGMDNNRMIHIREGLKVGEEVMLAPPVKEQKKEAVEEAVPPGGAPARSENRN
                     GSSAKPRRASAPSPARRGEGPPAGGRMP"
     gene            8898..9617
                     /locus_tag="PK576_04465"
     CDS             8898..9617
                     /locus_tag="PK576_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60877.1"
                     /translation="MQPTAWAASDPDAVVRLDMVKRHYRVGDETVRALDGVSFVIRRG
                     SYWAIMGQSGSGKSTLLNILGCLDRPSSGAYWLNGVNVAEMDDDSLSDHRLRNLGFVF
                     QSFHLIPQLTVCENIEVPMLYLGLSQTTRRAKAEQLAERVGIGHRLRHLPSELSGGQR
                     QRVAVARALANDPAVLLADEPTGNLDSATSVQIMELFQELYEQGKTVVLVTHEADIAA
                     YAKASIHLKDGKILSVTEAEA"
     gene            9614..10885
                     /locus_tag="PK576_04470"
     CDS             9614..10885
                     /locus_tag="PK576_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE60878.1"
                     /translation="MNQITYFWPFVKLGLTDVWRHKTRSFLTMLGMVFGVGSVIAMLA
                     VGEGASQQALESIKRLGSENIMVLSVKPGSEESGSSGAQRGKLAVYGLLYDDEKRIRE
                     TVPGVVMTVAARMTRRTARLNERQLEMRVVETVPEWFQVVQRPILAGRVLTAHDMRIR
                     ANVCVLTEHGVRRLLAAEETIGQRIRIASGVFEVIGIVQNETGGSVRAPDSEADAYIP
                     ITTGTKFFGEANIRYSAGGMEGEKVELSQIIVKMADTSVVESGSKAIQAVLERFHKKK
                     DFRIDVPLTLLREAERTKRTYNIVLGAIAGISLLVGGIGIMNIMLASVTERTKEIGIR
                     RAIGARRSRIVVQFLINTCVLSIGGGFVGLILGVAIPFLITLFTDMPTVVQPYSLVLA
                     FGISAGIGIVFGLYPAMRAAALDPIEALRRD"
     gene            11064..12116
                     /gene="sppA"
                     /locus_tag="PK576_04475"
     CDS             11064..12116
                     /gene="sppA"
                     /locus_tag="PK576_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOE60879.1"
                     /translation="MSDNRDTSKTGRGCGFGCALAIGMFIGFGLLVAVLVTGSFHVLG
                     VAGEHGAMEWFRSFRTRPEAGVDEAPNLIETWSAGDGEVKVVRIPLNGMIMLGDASWY
                     AGNAAAALRAIRKARHDPEVAGLILEINSGGGGITDSDILYQALCDFKTSSKGRVVVS
                     LLGDVAASGGYYVALASDVILAHPTTLTGSIGVLLQAYNIQELAAKIGIRDVTVKSGE
                     NKDLFNPFQEVKSTQREMVQKIITAMHERFVGLVAKNRTLPLETVRTLADGRIFLADE
                     ALRHKLIDGIGYQADAQSRIAQLLETDAVKVFRYEEQVTLMDLFTRRGIGLQTDLGRF
                     LRDDARDARLLYRWSW"
     gene            12130..14469
                     /gene="murC"
                     /locus_tag="PK576_04480"
     CDS             12130..14469
                     /gene="murC"
                     /locus_tag="PK576_04480"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOE60880.1"
                     /translation="MNVKTGEWRVPGRVHLLGIGGVGMAGLAYLLARQGWRVSGCDAR
                     PNALCRWLEEAGIPVAGKHAPEHLDEAERLIVTPAVAPDEPEWVQARARGMPVFFRGE
                     VLAAWVSSRRGVAVCGAHGKTTTACFATRLLGMLGPSPAWCVGGATRAMGKVAWEGAE
                     GQEVFVAEADESDGTLALYRPAVTVVTNMDLDHLDHFANEEALLNCFRAVAAQTREGV
                     AFCRDDPRATLAAQAATVPVLGFGFGEAADLRACAVEAGAESVAFTPVYRGREQARVV
                     LCVAGRHNVLNALGAAAAALILGCDPAAVFAALPQACGELPGRRFECVSDEGGIRVVT
                     DYAHHPVELEAAVAMARLQEPSRLVVVFQPHRYSRTKALAHAFPQALKGADEVILLPV
                     YAASEPWIEGGDSADLYAHFREAGMGDTRVMLARSQEEAWQFLRQTLQPGGLLLLAGA
                     GDVIGMVDGIREDRQRGWPACRDPEGFEKRVRAIEGLHVTPFGTLSEWSFFRTGGWAR
                     WRVEAADETALGALLKQCRQDDVPWRFVGAGANSWFSDLGEPGCVIRLAASACREWET
                     AGDEIRVGCNWKGAALLDELERRGLTGLEFLDSVPGSLGGWLAMNAGAHGGEIGERVR
                     WIRCLNSDGEITILHPMDFGFGYRRCGGLTGCIALSCALRVERADAGAVRVRREAFRA
                     RRISLAGLRTEGSVFRNPPGDAAGRLLDTAGCKGMRVGGARVTARHANIVAVEDPVTA
                     SDVLALVTRMRRRVEQVWGVSLSPEICGLTGGVTEEGIG"
     gene            14466..15362
                     /locus_tag="PK576_04485"
     CDS             14466..15362
                     /locus_tag="PK576_04485"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOE60881.1"
                     /translation="MKRIAVMMGGTSSEREVSLRSGEAVVAGLREAGFDAVPVVLNAD
                     RIDTLPSAVDAVFLALHGGYGENGGVQSDLDRLGLPYTGPGAAASRIAMDKIATKRVL
                     EAAGVPTPAFEVLSRGMEDFSLPLPLVVKPPRDGSSVGVVKVTSADAWPAALEQARAK
                     DPRGEVLVEAYIDGREWTVSVLDGEALPVVEIEAPGGWYGFTEKYTSGMTRYTFPEAV
                     EDAPLAAQCRVLALLAFQAVGCRGVSRVDFRVTSSGEAYVLEINTIPGFTATSLLPKA
                     AAKAGLSFSELCARLIGTATCG"
     gene            15359..16255
                     /locus_tag="PK576_04490"
     CDS             15359..16255
                     /locus_tag="PK576_04490"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOE60882.1"
                     /translation="MRRGTKRMAWDFFKQGGTQRKRMQAKGRRVTGVVTDLPPGVRFA
                     IRVAAGMVILLVVTWGGWRLFEAYYFCSRRLFVLHNLRENVMITTGRTLTPDLICEVL
                     GLREGVNLFSIPIRKKRKELLEQAPNIRDITIVRRMPDKIRINIIEREPIARVGSNGR
                     VVDEEGVVFIRYAGTGGLPMIKGVDAFEQIKPGDRLRGNELAAVRLVNNAQRPECRLR
                     LLVVDTSKEDYLLLTLFDHRQVKFAWKGMRDEAKDTVSRMQRHFDQLAQAMESEIGRA
                     CLMWDATQPGRIFATPSGLNEP"
     gene            16274..17476
                     /gene="ftsA"
                     /locus_tag="PK576_04495"
     CDS             16274..17476
                     /gene="ftsA"
                     /locus_tag="PK576_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOE60883.1"
                     /translation="MAIPPIAALEIGTSRTVLCVGESDDRGRVKITGVGTYPTTGVRK
                     GQVFDLAQARVGVELAAKQAEKQADVSIWQVLLAASGGHIQAVANPGMVPIRSGDRVV
                     TQEDVEEVTENAQTIQLDADRQVLHTINQSFTVDDQPGIVKPVGLRGKILTLNVMAVH
                     GLRNHIENAISVAKSAQLEVTDVAFSGICAGLAVLTPEQRRRGVVLIDLGGGTTNYLA
                     YGNGVVSALGCVAVGGDHITNDIALAFTIPLNRAEELKCAEGCALIEPESGTRRITLS
                     ADIGFEERVLSCKALHTVIHARMDETFRIVRSKLDEGGVLPHLGAGVILTGGGAYLRK
                     ATDLAQRIFGLPCGIGLPVNVSGLDNVPQAAALSTAAGLVLYGRMTYEDRGILSPLKN
                     LFKGVFRR"
     gene            17497..18648
                     /locus_tag="PK576_04500"
     CDS             17497..18648
                     /locus_tag="PK576_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60884.1"
                     /translation="MLLMGIGGGGCRLAAMVQSTYGDGIRALGMDTDALSIRTASEGG
                     LTCLLLGGSRLTGHGTGGDAIMGRLAVQDDLPNLTAHIQGVRSVVLVTCLGSGTGGGA
                     TPEVVKALHSQGIATLCFVTLPFAFEGENRRKAAERVLPMIEEHADSVVVLPLDDLFA
                     DTREPVLATALEAADRVLATAVTLLWRLVSTPGFIALDEERLHAMVVKGGNARFGFAS
                     AAGPDRALRAAEMLRECRTLRHGDALSKANALLFGILAGRDLRLAEIGDLMERMRACC
                     KKECVVEMGTVLDPDFEGRVELVALAFESWTAASLVAPIPDVPAGHEPPVADSFPIRP
                     GGRRSRSKGSKLSFGATGRGKFDQVEPTLHSGQDLDIPTFMRRGITLER"
BASE COUNT         3566 a         4932 c         6657 g         3722 t
ORIGIN      
        1 gcttgcctat agccgtcaga acgaatattg ttttgaacgt gcgtggaatt attcgcgggc
       61 gagacaggct caacgacact gtccggcacg cggtaagaac accaatacgg ctttggaaga
      121 ccgcagaaat gatagttttg ggacacattc ttttcggtct cggtagtttg gcgtgtttta
      181 ttggtgagat taagatgtta actcttgcct acaggcgcgg tccgggctgg ttcttgagtt
      241 gcatctttct tgccccgata tgttggctgg cattgcttgc agtggactgt aaactcgcta
      301 taaaaccgtt tgtgctggca tgtgctgggc taattgccat tggaattggc ggttcattgg
      361 cagggataga attcggatga cttcaaccga agcctaacta gcggcgacac tgaacggagc
      421 tacgacgtgg cgcgatgacg cctgccgaaa tcgaaggtga aatccgcgcg gcgcgtgacg
      481 cccgcctgta ctcaattgac tgacacagag aagtgtttga aactgtgtgc ggaggtggag
      541 agtgatgaag aaactggcca tagtcattct gggtgcgccc aacgacgccg agggaaatct
      601 ttcgtctatc gcgatcgagc gatgtcagcg cgcgttgact gagtatgcgg cgaatcccga
      661 cgccaagatg ctgccgacag gagggtgggg agaccacttt aacacaacgg acaaatctca
      721 cggctattac actcggcagt acctgatggc gcacggagtt cccgagagga acattctgga
      781 gtgtgctgaa agcgccaaca caatccaaga cgcgcaactt gtcaggccga ttgtcaggcg
      841 tcacgagatc gatgcgttaa tcgttgtcac gtctgatttc catatcccac gggcaaggtt
      901 cctgtttgag cgtgagtttc cggacctccg gctatcattc tcggcggccc agacgcatct
      961 gcccgaggag gaactcggac ggctaaagga acacgaggcg aaggccttgg ccagactgaa
     1021 gcagagcaac gccattggga actaacccgt gctctgtcgc gcaatgtgcc cggcgttcgt
     1081 gaagaaagca aaaccgcgag ggataatagt caacacaaaa tatgcgcttg cccatagccg
     1141 tcagggtgaa tattatcctg aacgtatatg gggtttgtct gctttcgcga ggcaggcccg
     1201 gtaacgctgt taggtttgag aaaatgagac tatgaagaac tgcacactag ccagactcga
     1261 cgaagacgag aaggcccgac agagcgtact ccctcattgc cgactggctc ccggcgaaat
     1321 atggatagac ccggtacgtg gccatcgcat cggatgcgtt gacgcaactg atgcccgaca
     1381 gatcgccgag ttgatggatg gacaaccggc ttctttggcc gtccatgatc ccccatataa
     1441 tctcgtcgcg ttcgggaaac gttctgtcgg cgatttcatc agctggtgtg ctcgatggat
     1501 tgagaccaca agcgcttccc tctgtgctga tgccagtctg tacgtctggc ttggtgccga
     1561 ccagaatgaa gggtttcagc cgttaccgga cttcatgttg atgatgcgcg gtttcaaaga
     1621 gttttcctcg cgatccttga tcaccatgcg caatcagcgc ggatatggga cgcagaagaa
     1681 ctggatggcg gttcgacaag agtgtctcta ctatacgaag gggagcccct cgtttgctgt
     1741 ccaatacacg gacattccca aaattcttcg tgggtactac aaagagatca acggtaagaa
     1801 gacggagaat atggaacgga gcaagtccga gaatatccgt ccggggaatg tctgggtgga
     1861 tattcaacaa gtattctacc gcatggaaga gaatgtgaac gggtgctacg cacagaagcc
     1921 catcaaggcg attgagcgcc ttgtcgcagc aagttcacgc gaaggtgata ctataatcga
     1981 cttcttcgga cactctggca ccacccttat tgtagcggag cagatgaagc gcaggtgcat
     2041 gacctgtgat gttgacccga tttattgtga aatcatgatt cggcgtctgg agcgtctgcg
     2101 cacgtatgga aagaccggat ggcagaacgg caacccattt gagaaagagc ttcacggtgt
     2161 tgtcgaaagc gaacaacccg tattggagct gtggtaatgg aaagactcaa aacgcaactt
     2221 gagacgctca tcttagaact cgaagactcg acggaaatct atgagcgact tgaaaatctg
     2281 atctctgtct acccgttcaa tgaatacgaa tacatggtct ccacactgat gggtcgaggc
     2341 aagctttccc ttgatgacta ctacgccctt cgagatgaat acattggacg gaatatgtac
     2401 ctgtacattt ttgaaatcag cgctccgaga ggcttcggcg agacatgggc acaagggcac
     2461 ctcaaggaac ttgttcccga acttcagaag ccatcaaaga aaaaagatcc ggcctattcc
     2521 ggacagtacg acttctgtct cgatgacagg atcgccatcg aggttaaagc ttcgcgtgcc
     2581 gtggacttcg aacttcaagc ccctctctat gtgaaggctc tcgcgtcgga ttccactcgc
     2641 ccatttgaca tgaacttcca gcaggtgaaa cccggttgtt gtgatgtctt cgtgtgggtt
     2701 ggcgtgtggc gtgacaagat tcggtactgg gtgctcgcgt cccgagaaat ggagtcaaac
     2761 cgttattact ccaagggaca acatcgtggt aacgttggtg aagggcaact gcacctgcga
     2821 cacgataaca ttgcggaatt cgctgactat gaggtgatcg gcagagacct tcttcaggcg
     2881 atcaaagctg cgtatgaccg gcaacatgca tgatgtccag caagacaaat tcagcggacg
     2941 tgccaccgca aacagcatgt gccggaaacc gccgaaatgt agccggaaca gccattatcc
     3001 ggctcggctt gaaaaaaatg gggcaagtcc gcgttacggt tggaattgat gttgcgggtg
     3061 gaatccggta aacttccccc tgttatccca tttcaggaaa aggggcgatc tcatgagcaa
     3121 gaaaaacagc gggactgcgt atcgaatcgc gtgtgaacgc tatgcggaga tgggggtcaa
     3181 cgtggaggcg gctctcggga agttggctca aatcccgatt tcgttgcact gctggcaggg
     3241 cgacgacgtg cgtggcttcg agaatcccga gggggatctc acgggcggga tccagaccac
     3301 cggtaattat ccggggcgcg cgcgcacccc gcaggagttg cgcggcgaca ttgcgttcgt
     3361 gctttcgctg gtcggcggct cgcaccgcgt gaacgtgcac gcctgttacg cggagttcga
     3421 gaagggcaag aaagtcgacc gcgacgcgtt gcagccggaa cacttcaaaa actggatcgc
     3481 ctgggccaag cagaacaggc tgggcatgga tttcaacccg accctcttct cgcatccccg
     3541 gtttgacgga ttttctctgg ccagccgcga caaacgcacc cggaatttct ggatccggca
     3601 cactcaggcc acgcggcgga ttgccgcggc tatcggcaaa gcactgggca cgcccagtgt
     3661 caacaacgtt tggataccgg acggctacaa ggacatcccg gctgaccgcg caaccccgcg
     3721 caagatcctg aacgacgcgc tggacgagtg tttcgcggag aagttgaacc ccagacacat
     3781 gctggatgcg gtcgaatcca aactcttcgg catcggtgcg gaaagttata cggtcggttc
     3841 ccacgagttc tacatggggt acgccgtgaa aaatcagaag ttgctgacct tggatgcggg
     3901 gcacttccat ccgactgaaa cgatctcgga caaactctcc gcctgcctgc cgtttgttcc
     3961 gggtattctg ctgcatgtga gccggggggt gcggtgggac agcgaccatg tggtgttggt
     4021 gggggacgat ctgatcgcga tcgcgcagga gttggtgcgg ctggaccagt tccggcgcat
     4081 acacatcggg ctggattact ttgacgcgag catcaaccgg atcgcggcgt gggtgctggg
     4141 catgcgcgcc atgcagaagg cgttgctgta cgctttgctg gagccgttcg agaccatgcg
     4201 cgaggcggag cgggaaggcg acctgaccgc gcgcctggtg tggactgaag ccgcgaagac
     4261 gatgccgttc ggcgcggtct gggaaaagtt ctgcctggag gaggacgtgc cgcctgatcc
     4321 ggcttggctc aatgaggtca aacggtatga ggcgcaggtg ctggcgaaac ggtgagcgga
     4381 gggcagtgcg tggggtggga gagtagagtg ttactcgaag cggttaccga actgtcgcat
     4441 gcgttcggat ccgcagcgta tgtcaaaggc ggcgggggca atacctcctg caagcaggcg
     4501 gacaccctct gggtgaagcc ctccggtacc acgctcgccg cgctgacccc cgaccggttt
     4561 gtcgcgctgg accgcgcccg gctggcgtgc ctctaccggg agacgccgcc ggacggagcg
     4621 gccgcgcgcg aggcttgggt caaggagcgg atggatgcgg cggtgcggcc agagacgccc
     4681 ggacgcgcct cagtggaggc gccgctgcac gacacgctcg cggcccgttt cgtggtgcat
     4741 acgcatccgg aggcctgtaa cggcatgact tgtgcggtcg gcggtgaagc ggctgcggcc
     4801 cgtctctttc ccgaggcgct ctggattccg tatgtggacc ccggttacac gttgtgccgg
     4861 gaggtgcggt gcgcggttca ggactatgcg cgggaacacg gtgcggagcc tgcgctgatc
     4921 tttctccaga atcacggtgt ggtcgtttca ggggacacgg tggaggcggt gcgtgcggtg
     4981 tacgagagcc tgatggaacg gttgtcccgc gagtatgcgg aggcgcatct ggacggcggg
     5041 gcgcacgttg gaccggagcc tgcggaacat gaggtggccg gttggcggca aacgctgtca
     5101 gagaccttcg gaagcgaggc ggcggcggtc gccgtatccg gcatgctgga tgtgggggcc
     5161 ggaccgattt ctccggacca tgtcgtgtat gcgaagtcgt atccgtttga agggcgcgtc
     5221 acaccggaga atctccgcgc gttccagtcg gtgcggggct atccgccgcg cgtcgtggtc
     5281 acgggttcgg cggtgctggg cgtcgggccg cgcgaaagcg tggcccgggt ggcgctggat
     5341 ctggcccgtg acggtgcgga ggtcaagcgg ttggcgcgcg cgttcggcgg gttgcggtat
     5401 ctgagtgacg cggcgcgcca attcatcgag aattgggagg ttgagtccta tcgggcgcaa
     5461 cagatgtgag cggaacgtgt tggggaaggt gtatgcgcgg gccgggggct tggggaagcc
     5521 gtagggatgt gcggacgaaa ggtttttttg gggggcaaat gaggcgtgcg ggatcatacc
     5581 tgcgaaaggt gcgtgtcgga cacgggttga ccgtgtttgg agtggcggga gtctgtctgc
     5641 tcgctccgat cgcctgcatg acgccggggc gggcggtgct ggagacggat gagaccggca
     5701 cccggttggc gaccgcctac tggcagcagc agaccggatc gaccaatacg ttcgacatga
     5761 gccgtccggc ggatacgctg acgttgcgca tcgcgttgct cgcggcggcg cgcggggagc
     5821 agaacgtcat cttccccgcc atcccgaact tgggcgaact gaacgtctcc aacgaagtca
     5881 ttgtgctcac gttgccgcat gccctgtgtc tggcggcaag gaatgaccgt tcgtaccaga
     5941 agctgaagga ggcggtgttt gacgcggcgc tggatctgga ttaccagcag taccggttcg
     6001 gcacgacctt cagcggtatg atgttcggtc tgctctccgg agatccgggg atcgaaaaag
     6061 ccagcggcgg ggtttccgct ggcgcggcca gacaaaccga ggccgggctg gcggttgccg
     6121 ggcggttggc cctgaatgtg gtcagcctgt tgcgtgacga ttggcgttcg atgggactga
     6181 ccggcgatct ttccatgacc cttccgcttc tgcgcggcgc cgggcgcgag atcgtgcgcg
     6241 aaccgctgac ccaggcggag cggaacctca cggtcgcgat ctggaacttc gaacgatacc
     6301 gccagacgta cgcgtattcg gtggccagcg cgttttataa cgtgttggaa tacgggcagc
     6361 gtctgaaaaa cgcgatcgac aatgagagcc gcctgcaaga aaacagccgc cgtgctgaaa
     6421 tgtggttcga ggcgggccgc atggagcgca tccaggtcga ccaggcgcgt tccgacctgc
     6481 tgaacgcggg cgagagcgtc atcaacaccc gtaagaatgt ggaagagaaa ctggacgctt
     6541 tcaaaatcca gatcggtttg ccaccggagg cgaagatcgt tttggatgag cgcgaactgg
     6601 acgagttgga gaaggagatg gagcggatag ctgaacgcag tccgaatgcg gtcgaagcgt
     6661 ttcccgaaga ggacgaggcg tgccgcatcg cgctggcgga gcggcatgac ttgctcatca
     6721 cgcggagcgc gctcgaggat gtggcgcgta gcgtgaagat cgcggcggat gccctgcggg
     6781 ccgatgtgac gcttcagggg gggggcagtc tggatcgcct gcgtgaaacc gggaggagcg
     6841 ggtttaaggg cggcgaggcg cttgaggggg ggctttcggt gtcgttgccg tgggaccggc
     6901 ggcgcgaacg caacgctttc aaaaagcgtc tgctcgagct ggaacaggcc aaacgggcgt
     6961 tggaggctat ggaggacacg gtgaaacagg cggtccgcag cggattccgg aatctgatcg
     7021 cggcccgcgc gtcgtatgag aaccagcagg agaccatgcg cgtggcccgt ctgcggttgg
     7081 aaagcaacaa tctctttctg ctttccggac gttcctcgat gcgtgacatt ctggaggcgg
     7141 agagcgcgat gctcacggcg cgcaatgcgt tatgctccgc cgtgatccag tggcggctca
     7201 gcgaattggc cctgcgccgg gatatgggcg tgttcgggat cggggaagac gggatgtggc
     7261 gcgaaacggt ggaccgaggc aatcatggtt aagaagacat ccaagaaaaa aagaatcgcg
     7321 gtcggcacgc ttctggtgct cggtgcggcg gcgtggttct ggttcggcaa acgggacaaa
     7381 accagcgaag ccgtgccggt ttttgccgtg cagcaggggc ctctgacgat cgcggtcacc
     7441 tcgggcggat cgatccagag ccgcgacaag atcgtgatcg ccagcgaatt agagggcaac
     7501 aacacggtga tctgggtgat cgacgagggg gtcagcgtgc agccgggcga tttgttgctg
     7561 gagttcgatt ccagcgacct catcgaaaaa cgtaacgagc aggagatcgt ggtggccaac
     7621 gcagaggcga acctgatcat cacccaggag aaactcggca tcaccgaggg ggactgcgaa
     7681 gcggcgctgc tggaccggga agtggatatg atgctggcca aaatggcgct ggaaaaatat
     7741 caggacggcg attatccgca gcaactgcgt cagtcggagg cggatgtggc gctggcggac
     7801 gaggagttga agcgcgcgga tgagcggttg gagtggtcgc agcggctcgc caaggcgaac
     7861 ttcctgaccc ggaccgaact gcaggccgac cagttggccc tgcagcgcaa aaagctcgat
     7921 ctcgccatgg cggagaccaa aatgagcgtg ttgaccaact tcacggtttt gcaacagcgc
     7981 gcgactctgg aaagcgcgtt gcggaaagcc acgcgcgcgc tgaaacgtac cacttggcag
     8041 aacaaggcta cgctgcgtca ggtggagacg gaactgcaag cgcggcaacg ggaactcaat
     8101 cgggccacca accgcatggc ggaactgaac ttccaaatcg agaagagcaa gatctacgcg
     8161 cccaccaacg gaatcgttct gtacacctcg accgtcctga tctcccggcg tcaatggtgg
     8221 atcaagccgc tggcggtggg ttccaccgcg gtggagcgtc aggagctgat ctacatcccg
     8281 ctggagtccg gaatggtggt ggaggtgatg gttcccgagg ccagtctgaa caagatcgaa
     8341 aacgggatgg aggcgcgtgt caaggtggac gccttcccgg ggcgtgtgtt tgaggggcac
     8401 ctggtcaaga tcgggatcct gccggatggc cagagttcac agttgaatcc ggatctgaag
     8461 ttgtacaaat gcgagatcga gtgcgatttc aaggaggtca acatccgtcc cggcatgagc
     8521 tgtgatgtcg aactggtcaa ggagacgtat gagaacgcgc tctacgtgcc ggtgcagtgc
     8581 gtggtccgcg tggagggcgt gccgcgcgtc tatgtgcgcg atgacggcga atgggtgccg
     8641 cggatcgtaa gcgtgggcat ggacaacaac cggatgatcc acatccggga agggctgaag
     8701 gtcggcgagg aggtcatgct ggcgccgcca gtgaaggagc agaaaaaaga ggctgtggag
     8761 gaggcggttc ctccgggtgg cgcgcccgcg cgttcggaaa accggaacgg ctcgtccgcc
     8821 aagcccagac gagcctccgc gccctcgccg gcgcggcgtg gggaggggcc gcccgcaggc
     8881 ggcaggatgc catgatcatg cagccgacag cgtgggcggc atccgatccc gatgcggtgg
     8941 tgcgcttgga tatggtgaaa cggcattacc gggtcggcga cgaaacggtt cgggctctgg
     9001 acggcgtttc cttcgtgatc cgccggggtt cctattgggc catcatgggc caaagcggtt
     9061 ctggaaaaag tacgctgctg aatattttgg ggtgtctgga ccggccttcg tcgggtgcgt
     9121 attggctgaa cggtgtgaat gtggcggaga tggatgatga ctcgctgagt gatcatcgtc
     9181 tgcggaatct gggtttcgtg tttcagagtt ttcatctcat tccgcaactg acggtgtgtg
     9241 aaaacatcga agtgccgatg ctgtatctgg ggctttcgca gacgacgcgc agggcgaaag
     9301 cggagcaact ggccgaacgt gtcggcatcg ggcaccggct gcgccatctg ccttccgaac
     9361 tgtcgggcgg gcagcgccag cgtgtggcgg ttgcccgcgc gctggccaac gatccggcgg
     9421 tcctgctggc cgatgagccg accgggaatc tggacagcgc caccagtgtg cagatcatgg
     9481 agttgtttca ggagttgtat gagcagggca agaccgtggt tctcgtgacg cacgaggcgg
     9541 acatcgccgc ctacgccaaa gccagcatcc atctcaaaga cggcaagatc ctctcggtca
     9601 cggaggcgga ggcgtgaacc agatcaccta tttctggccg ttcgtgaaac tcggtctcac
     9661 cgatgtgtgg cggcataaga cccgctcgtt tctgacgatg ctcggcatgg tgttcggtgt
     9721 gggcagtgtg atcgcgatgc tggcggtcgg tgaaggggcc agtcagcagg cgctggagtc
     9781 gatcaagcgg ctgggcagtg aaaacatcat ggtgctctcc gtgaaaccgg ggtccgaaga
     9841 gagcggcagt tccggcgctc agcgcggcaa actggccgta tacgggttgc tgtacgacga
     9901 cgagaagcgc attcgtgaga cggtgccggg tgtcgtgatg accgtggccg cgcgcatgac
     9961 gcgccggacc gcgcgtctga atgagcgcca actggagatg cgcgtggtgg agactgtccc
    10021 cgagtggttt caggtggtgc aacggccgat cttggccggc cgggttctca cggcgcacga
    10081 catgcggata cgcgcgaatg tctgtgtcct gaccgaacat ggcgtgcggc ggttgctggc
    10141 ggccgaggag accatcggcc agcggatccg catcgcttcg ggcgtcttcg aggtcatcgg
    10201 catcgtgcag aacgagacag gcgggtcggt ccgcgcgccg gacagcgagg cggacgcgta
    10261 catcccgatc accaccggaa cgaaattttt cggcgaagcc aacatccgct attcggcggg
    10321 cggcatggaa ggcgagaagg tcgaactcag ccagatcatc gtcaagatgg cggacacctc
    10381 cgtggtcgaa tccggcagca aggcgattca ggcggttctg gagcggttcc acaagaaaaa
    10441 ggatttccgg attgatgtgc cgcttacgct gctgcgtgag gcggagcgca ccaaacggac
    10501 gtataacatt gtgttggggg cgatcgcggg catcagcctg cttgtgggcg ggatcggcat
    10561 catgaacatc atgttggcgt cggtgacgga gcgcaccaaa gagatcggga ttcggcgggc
    10621 gatcggcgcg cgccgcagcc ggatcgtggt gcagtttttg atcaacacct gcgtgttgtc
    10681 gatcgggggc gggttcgtgg ggttgattct cggcgtggcg attccgtttc tcatcaccct
    10741 cttcacagac atgcccaccg ttgtgcagcc gtacagtctc gtgctggcgt tcggcatcag
    10801 tgcggggatc gggattgtgt tcggcctgta tccggcgatg cgggcggcgg ccctggatcc
    10861 gatcgaggcg ttgcggcgcg attgagaaac atggcggtat ggacggggcc atgcccttcc
    10921 gtgcgcacga cggcgtttta cacaaaacgc gccctccccg cgcatctctc gtcatcattc
    10981 gattcccttt cgggtaacgc tttgccgtgc cgggagggtc tgctatactg taccgtgtat
    11041 cgggttgaaa aagggggacg cacatgtcag ataatcggga cacatcgaaa acgggacgcg
    11101 gatgcgggtt cggctgcgcg ttggcgatcg ggatgttcat tgggttcggg ctgctggtcg
    11161 ctgtgctggt gacaggaagt tttcacgtgc ttggcgtggc aggggaacac ggggccatgg
    11221 agtggttccg ctcgttccgc acgcgtccgg aagcgggtgt ggatgaggcg ccgaacctga
    11281 tcgagacttg gtctgcgggc gacggcgagg ttaaggtcgt gcgtatcccg cttaacggga
    11341 tgatcatgct gggggatgcc tcgtggtatg cggggaatgc ggctgcggct ctgcgcgcca
    11401 ttcggaaggc caggcatgac ccggaggtcg cggggctcat cctggagatc aacagcggcg
    11461 gcggcggcat cacggatagc gatatcctgt atcaggcgct gtgtgatttt aaaacatcgt
    11521 ccaaaggccg tgtggtggtc tcgctgctcg gggatgtggc cgcttcaggc ggctattacg
    11581 ttgcgctggc gtcggatgtg attctggccc acccgaccac gcttaccggt tcgatcggcg
    11641 ttctgctgca agcgtacaac attcaggaac tcgccgcaaa gatcgggatc cgggacgtta
    11701 ccgtgaaatc cggggaaaac aaggatctct ttaatccgtt tcaggaagtg aagtccacac
    11761 agcgcgagat ggtgcagaag atcatcaccg cgatgcacga acggtttgtg gggttggtgg
    11821 cgaaaaaccg aacgctgccg ctcgaaacgg tccggacgct ggcggacgga cgcatctttc
    11881 tggctgacga ggcgctgcgg cataaactga tcgacgggat cgggtatcag gcggacgcgc
    11941 aaagccggat cgcccaactg ttggagacgg atgcggtcaa ggtcttccgg tatgaagagc
    12001 aggtcacgct gatggatctg tttacgcgtc gggggatcgg tctgcagacc gatctggggc
    12061 gttttctgcg ggatgacgcg cgggacgccc gtctcctgta ccgatggtca tggtaaggtg
    12121 gaaccgcgcg tgaacgtgaa aaccggagag tggcgggtgc ccggtcgtgt ccatctgctg
    12181 gggatcggcg gagtcggtat ggcggggctc gcctatctgc tggcgcggca gggttggcgc
    12241 gtgagcggtt gcgatgcgcg gccgaatgcg ctttgccggt ggctggaaga ggcgggcatt
    12301 ccggtggccg gaaagcacgc cccggagcat ctggacgagg cggaacgcct gatcgtcacg
    12361 ccggcggtcg cgccggacga gccggaatgg gtgcaggctc gtgcccgggg gatgccggtc
    12421 ttctttcgcg gggaggtgct ggcggcatgg gtttcatcgc ggcgcggtgt tgcggtttgc
    12481 ggggcgcacg gcaaaaccac gaccgcatgt tttgcgacgc ggctgctcgg gatgttgggg
    12541 ccttcgcccg cctggtgtgt cgggggcgcg acgcgcgcca tggggaaggt cgcgtgggag
    12601 ggtgcggagg gtcaggaggt gttcgtcgcc gaagcggatg agagtgacgg tacgctggca
    12661 ctgtaccggc ccgcggtgac ggtggtcacc aacatggatc ttgaccatct cgaccatttt
    12721 gcgaacgaag aggcgctgct caattgtttt cgtgccgtgg cggcgcagac ccgggagggt
    12781 gtggcgttct gtcgcgacga tccgcgcgct acactggcgg cgcaggccgc gaccgttccg
    12841 gtattgggtt tcggtttcgg ggaggctgcc gatttgcggg cctgtgcggt ggaggctggg
    12901 gcggagagcg tcgcgttcac cccggtttac cgtggccgcg aacaggcgcg ggtggtgttg
    12961 tgcgtcgcag ggcgccacaa tgtcctgaat gcgttgggcg ctgctgccgc agcgctgatt
    13021 ttggggtgcg atccggctgc ggttttcgcg gccctgccgc aggcatgcgg cgaattaccg
    13081 gggcggcgct tcgaatgcgt ttctgacgag ggcgggatcc gcgtcgtgac agattacgcg
    13141 catcatccgg tggagttgga ggcggcggtg gcgatggccc ggcttcagga gccgtcacgg
    13201 ctggtggtgg tgtttcagcc acaccgctat tcgcgcacaa aagccttggc gcacgctttc
    13261 ccccaggcgt tgaaaggggc cgacgaggtg attcttctgc cggtctacgc agcatcggag
    13321 ccgtggatcg agggcgggga cagcgccgac ctgtacgcgc atttccgtga ggccgggatg
    13381 ggggacacgc gtgtcatgct tgcgcgctcg caagaggagg cgtggcagtt tttgcggcag
    13441 acgctgcaac cgggcggcct gttgctgttg gcgggcgcgg gcgacgtgat tggcatggtg
    13501 gatgggatcc gcgaggatcg gcaacgggga tggcccgcct gccgtgatcc ggaggggttt
    13561 gagaagcgcg tgcgtgcgat cgaaggactt cacgtgacgc ccttcggcac gttgtcggag
    13621 tggtcgtttt tcaggacagg cggttgggcg cgctggcggg tggaggcggc tgacgaaacc
    13681 gcgttgggtg cgctgctgaa gcagtgtcgg caagacgatg tgccgtggcg gtttgtaggc
    13741 gcgggagcga acagttggtt cagcgatctg ggcgaaccgg ggtgcgtgat ccgtcttgcg
    13801 gcgtccgcct gccgggagtg ggaaacggca ggggacgaga tccgggttgg atgcaactgg
    13861 aagggggcgg cgctgctgga tgagttggag cggcgcggat tgacggggct cgaatttttg
    13921 gacagcgttc ccggttcgct gggggggtgg ctcgccatga atgcgggcgc gcacggcggg
    13981 gaaatcggcg aacgggtgcg ttggattcgt tgtttgaatt cggatggcga aatcactata
    14041 ctgcacccta tggatttcgg tttcggctat cggcggtgtg gcgggcttac agggtgtatc
    14101 gccttgtcgt gcgctctgcg tgtggaacgc gccgatgcgg gtgccgttcg ggtccggcgt
    14161 gaggcgttcc gcgcccgccg gatttcgctg gcggggctcc gaaccgaggg gtcggtgttc
    14221 cgtaacccgc cgggtgatgc ggcgggacgg ttgctggaca cggcgggatg caaaggcatg
    14281 cgtgtgggag gggcccgggt aacggcgagg catgccaata tcgtggcggt agaggatccg
    14341 gtgaccgctt cggatgtgct ggctttggtt acgcggatgc gtcggcgtgt cgagcaggtc
    14401 tggggcgtgt cgctgtcacc ggaaatctgc gggctgacgg gaggggtaac ggaagaggga
    14461 atcggatgaa gcggatcgcg gtcatgatgg ggggcacgtc aagtgaacgg gaggtttcgc
    14521 tccgatcggg cgaggcggtg gtcgccgggt tgcgcgaagc cgggtttgat gcggtgccgg
    14581 tggtgttgaa tgccgaccgg atcgacacgt tgccgtccgc tgtcgatgcg gtctttctgg
    14641 cgttgcacgg cggttatggc gaaaacggcg gggtgcagag tgatctggac cgtctcggtc
    14701 tgccttacac cggaccgggc gcggccgcga gtcggatcgc gatggacaaa atcgcgacca
    14761 agcgcgtgtt ggaggcggct ggtgtgccga cgccggcgtt tgaggtgttg tcgcgcggca
    14821 tggaggattt ctcgctgccg ttgccgctgg tggtgaaacc gccgcgggac gggtcgagtg
    14881 tgggagtcgt caaagtgacg tccgccgatg cgtggcccgc cgcgttggag caggcgcgtg
    14941 cgaaggatcc gcggggcgag gtgctggtcg aggcgtatat cgacggacgc gaatggacgg
    15001 tgagcgtgtt ggacggtgag gccctgccgg ttgtggagat tgaggcgccc gggggctggt
    15061 acgggttcac cgagaaatac acctccggca tgacgcggta cacctttccg gaggcggtcg
    15121 aggacgctcc gctggcggcg cagtgccgcg tgttggccct gctggctttt caggcggtgg
    15181 gttgccgggg tgtcagccgg gtggatttcc gtgtcacctc ttcgggcgag gcctatgttt
    15241 tggagatcaa caccattccg ggattcacgg ccaccagtct gttgcccaaa gcggcggcca
    15301 aggcgggact gtcattctcc gagctctgcg cgcggttgat cgggacggcg acatgcggat
    15361 gagacgaggc acgaaacgta tggcatggga ttttttcaag cagggcggaa cgcaacggaa
    15421 gcgcatgcag gccaaggggc ggcgtgtgac gggcgtggtc acggacctgc cgcccggagt
    15481 ccggttcgcg atccgggtgg ctgcgggtat ggtaatcctg ctcgtcgtca catggggcgg
    15541 ctggcgcctc ttcgaggcct attatttctg ttcgcgccgg ttgtttgtcc tgcacaatct
    15601 gcgcgagaat gtgatgatca cgaccggcag gaccctgacg ccggatctga tctgcgaagt
    15661 gctggggttg cgcgaggggg tcaatctctt ttcgattccg atccggaaga aacgcaagga
    15721 gttgctggag caggcgccca atatccggga tatcacgatc gtgcgccgga tgccggataa
    15781 gatccggatc aacatcattg agcgtgaacc gatcgcccgg gtggggtcga acggccgggt
    15841 tgtggatgaa gagggggttg tgttcatccg gtatgcgggt accggcgggt tgccgatgat
    15901 caaaggggtg gacgcgtttg agcagatcaa gcccggtgac cggctgaggg ggaacgagtt
    15961 ggctgcggtg cggctcgtga acaacgccca gcggccggaa tgccgtctcc gcctgctggt
    16021 ggtggacaca tcgaaagagg attatctgct gttgaccctg ttcgaccacc gtcaggtgaa
    16081 gttcgcttgg aagggcatgc gggatgaggc gaaagacacg gtgtcacgca tgcagcgtca
    16141 tttcgaccag cttgcgcagg ccatggaaag cgagatcggc cgcgcttgtc tgatgtggga
    16201 tgcgacacag cccggacgga ttttcgcgac gccgtccgga ttgaatgaac cgtaaccgta
    16261 gggaagggat ggtatggcga ttcctccaat tgcagcactg gagatcggta cgtcacgcac
    16321 ggttttgtgc gtgggcgagt ctgatgaccg tggacgggtg aaaatcaccg gtgtcggaac
    16381 ctatccgacg accggggtgc gtaaaggcca ggtgtttgat ctggcgcagg cgcgcgtggg
    16441 tgtggagctc gcggccaagc aggcggaaaa acaggcggat gtcagcatct ggcaggtgct
    16501 gctggcagct tccggcggcc atattcaggc cgtggcaaac cccggcatgg tcccgatccg
    16561 gtcaggcgat cgcgttgtga cgcaggagga tgtcgaggaa gtcacggaaa acgcgcagac
    16621 gatccaactt gacgcggacc ggcaggtgtt gcacacgatc aaccagtcct tcacggtgga
    16681 cgaccagccc ggcatcgtca agccggtcgg gttgcgcggc aaaatcctga cgctgaatgt
    16741 gatggcggtg catgggttgc gtaatcacat tgagaatgcg atcagtgtgg cgaagagtgc
    16801 acagcttgag gtgacggatg tggccttttc cggcatctgc gccggattgg cggtgctgac
    16861 tcccgaacag cgccgcagag gagtggttct gatcgatctc ggcggcggca ccaccaatta
    16921 tctggcttac ggcaacgggg tggtctcggc gcttggctgt gtggcggtcg gcggggatca
    16981 catcacaaac gacatcgcgt tggccttcac cattccgctc aaccgcgcgg aggaactcaa
    17041 gtgtgcggag ggatgcgccc tgatcgaacc ggaaagcggg acccgccgca tcactctgtc
    17101 cgcagatatc gggtttgagg agcgggtgct gagttgcaag gcgttgcata ccgtgatcca
    17161 cgcgcgtatg gatgagacgt tccgcattgt gcgttccaaa ctggacgagg ggggggtgct
    17221 gccgcatctc ggtgcgggag tgattctgac cggcggcggc gcgtatctgc ggaaagccac
    17281 tgatttggcg caacggattt ttggcctgcc ctgcgggatc ggcttgccgg tgaatgtcag
    17341 cggactggat aacgtgcctc aggctgcggc cttgtccact gcggccggac tggtgctgta
    17401 tggccggatg acctatgagg accgcgggat actctccccg ctcaagaatc tcttcaaggg
    17461 ggtgtttcgc cgatgaatca ggaaccgatc tcaggcatgc ttctgatggg tatcggcggc
    17521 ggcggttgcc gtctggccgc gatggtgcaa tcgacgtacg gcgatggcat acgtgcgttg
    17581 gggatggata ccgatgcgct ctccatccgc acggcctccg agggcgggct cacctgtctg
    17641 ctgctgggag gttcgcgtct cacgggacat ggaacagggg gtgacgcgat catgggacgg
    17701 ctggcggttc aggatgattt gccgaatctg accgcgcaca tacaaggcgt ccggagtgtc
    17761 gtgctggtga cctgtctggg ttccggcacc ggcggcggcg cgactccgga ggtggtcaaa
    17821 gctctgcaca gtcagggaat cgccacgctc tgtttcgtta ccttgccgtt cgcgttcgaa
    17881 ggcgagaacc ggcgtaaagc ggcggagcgc gtgttgccga tgattgagga acacgcagat
    17941 tcggtggtgg tgctgccgct ggatgatctg tttgccgata cccgtgagcc ggtgctggcg
    18001 accgcgctgg aagccgcgga ccgggtgctg gccactgcgg tcactttgct gtggcggctc
    18061 gtgtccacac cgggattcat cgcgctggat gaggaacggc tgcatgcgat ggtggtgaaa
    18121 ggcggaaacg cccgcttcgg tttcgcaagc gcagcgggac cggaccgcgc gctgcgggcc
    18181 gcggaaatgt tgcgggaatg ccgtacgctg cgtcatggcg atgcgctcag taaggcgaat
    18241 gccctgctgt tcggtattct ggcgggacgg gatttgcgtc tggccgagat cggtgatctg
    18301 atggagcgga tgcgcgcgtg ctgcaaaaaa gagtgtgttg tggagatggg caccgttctg
    18361 gatccggatt tcgagggacg ggtcgaactg gtcgcactgg cattcgaaag ttggacggcc
    18421 gcctcgttgg tcgcgcccat ccctgacgtg cccgctgggc acgagcctcc ggtggcggat
    18481 tcgttcccga tccggcccgg tggccgccga agccgttcca aaggaagtaa actctccttc
    18541 ggcgcgaccg ggcgcggtaa gttcgatcag gtggagccca cgttgcactc gggtcaggat
    18601 ctggatatcc ccacgttcat gcgccgcggg atcacgctgg aacgatgagc atcgccgatg
    18661 atcaagtgtc cagtcagtta caaatgggct gtggccctgc tccttgctgc cgccgccgcg
    18721 gtttcagggt gccgcatggt ccagtccgcg gctgatgcgc cgggacaggc tgtccgcgcc
    18781 gtcactcccc ccagcgctca ggccgaagaa ggccccgacc ccgtcgagat tcagtcgttg
    18841 gtcatgcgct ttggcgacga ttttatcagt cgcttga
//
LOCUS       DAOSLE010000042        18793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3172_length_18793_cov_7.732095, whole genome shotgun sequence.
ACCESSION   DAOSLE010000042 DAOSLE010000000
VERSION     DAOSLE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18793
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3172_length_18793_cov_7.732095"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            561..1250
                     /locus_tag="PK576_04505"
     CDS             561..1250
                     /locus_tag="PK576_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60885.1"
                     /translation="MKKLLYIVYVFVAVLTLTVALSMLNAKHEKMNTVATVGGAAISE
                     TNDALSIIVKTLLEQFDKDENIAVSPQEIEKQREFFVKLAKMIYDQRITYLNKELEML
                     NVSGDGTVDVSNKTEKIRKEMAWLEPSFFVPADDVLRTNVLLWKRNKALYKRYGGNVY
                     FSHVGPFPADAYKAFFKEKEAERAFEIFDEKLRSDFWGEQFNYTKQSDLLLDDGKKAI
                     ETPPWEATIDG"
     gene            1373..2812
                     /locus_tag="PK576_04510"
     CDS             1373..2812
                     /locus_tag="PK576_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019597491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60886.1"
                     /translation="MMSRTKTALPHRKAATRRGFLKRTLAASTLTAFPAIRATGRPVT
                     GDKVRLACCGIGNQGASDINNLYATGLCDIVALCDTDMGAPHTRKILDTFPDAPRFQD
                     FRKMLDKLGGQIDALCIGVPDHTHFPIAMMAMGLGKHVYVEKPMAHTFREIELMMKAE
                     KKNRVAAQMGNQGHSGANYFQFKAWTEAGIIRNVTRIVAHMNGARRWHKWNGVVTSLR
                     PAQPVPETLDWDTWLGQVPFHGYHRDYVHGDWRCWYSFGNGALGDWGAHILDTAHQFL
                     ELGMPTEVSALNLEGHNPYVFPMASTLSFKFAARGAMPPVEVLWYDGVKNFPELPPGF
                     GGSAKTADDIPTPGGGSTAVSKLSPGKEIYAEDLTFKGGSHSSTLEIIPAEKARDMAN
                     RLPEYPKNQSNHYKNFLLACMGRETCRSSFEVAGPLSQVLALGCIAQRLNTTFTLDPV
                     TRRITGNKLANDLLDGPPPRKGWEQYYRV"
     gene            2860..3381
                     /gene="ybeY"
                     /locus_tag="PK576_04515"
     CDS             2860..3381
                     /gene="ybeY"
                     /locus_tag="PK576_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE60887.1"
                     /translation="MSSPNIYIRIGTRRFRLDTAALKRLAAALAKRAQTRLRNGPVWR
                     EITIHLLDDAGITPVNAAILKHAGPTDVITQRYEPIPGEPDGLIGELFVNVERAARAA
                     PRRAGWNADRELALYLAHGFDHLAGAEDRTPAERARMRRRELAWLRHLSLTPLFRSGT
                     IPIQNRSTREFSI"
     gene            3378..5108
                     /locus_tag="PK576_04520"
     CDS             3378..5108
                     /locus_tag="PK576_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOE60888.1"
                     /translation="MNGALLLSLCGVLFLLAYTMYGRLIERILGVDPERPTPAHTLRD
                     GIDYVPTHPAVLFGHHFASIAGAGPIVGPVLAAQFGWVAVALWVVLGCIFIGAVHDLA
                     AIFLSIRHQGRSIGTVIESLMGYWGRMLFLFFCWFTLVLVVAEFLRIVAETFVEIPAV
                     GTASMLFIALAVGFGRLVYRGGMSVLTGSLIFVPLTFACVYFGTLIPLDLVKLLGCDA
                     AVARQIWVVVLLVYCFAASVLPVWLLLQPRDYLNSYLLYAMMALGFVGILVARPALNL
                     DAFTGWHAVSPLSGRSDPLIPLLFVTVACGACSGFHALVASGTTAKQIDNERHLRPVA
                     YGGMLIEGVLAIIALVAVGVMTQGEYTVALKEQGAVKLFAGGVASFTDKLGLPHEAGI
                     IFISLALAAFLMTTLDTATRLARFTWQELFLPRGQSGDVPPATVAPTLPASHAFFSNR
                     YIATLIAVVAAGWLLLGGGAKSIWPVFASSNQLLAALTLLGCSLWLLRHKRPLALTLL
                     PMLFMMATSGAAIVTLFLRNAQAWQADGFKAGGVMTLTTGILIVMSVALIVMGAVSIR
                     QTMRQSERKV"
     gene            5111..6280
                     /locus_tag="PK576_04525"
     CDS             5111..6280
                     /locus_tag="PK576_04525"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOE60889.1"
                     /translation="MAKELAFVLINPYSIRKSRTGGIIARYLVRTDLKLIGARVIGAS
                     AELVNAFATYLETYDPDDPEKCALLANYVRRFHMPDAATGRPHRSILLLFEGDDAIRK
                     IFDVTGSIRQQWCSGQSVRDTYGDLVRNPATGEVEYFEPAVLVGPSQKVVGDILRMWL
                     PHLASQAGIVRGALDVPGDADAQETLVMLKPDNWRQPSLRAGNIMDLLSNSGLRIVGV
                     KKFSMSVAQAEEFYGPVREGLKKVFKRFAADRAAQALSREFGFPVDAAPLDGLCDMLA
                     PSFAEREFESIVEFMTGRRPSNCLPEERTLPGTEECLAIVYEGIDAVSKIRDILGATD
                     PLKARPGSIRREFGTNIMVNAAHASDSVENAHREMRIIDVARDPHFEELIQNYYP"
     gene            6277..7443
                     /locus_tag="PK576_04530"
     CDS             6277..7443
                     /locus_tag="PK576_04530"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE60890.1"
                     /translation="MKKLNDAILAIKPSQTLAISSLAKQMAEQGCHVCNFSAGEPDFD
                     TPDLIKEAAITALRQGKTKYTPVAGVPQLCKALSAKFARDNGLDYKPSQIVVSCGGKH
                     SLALVFQTLLNPGDEVLIPTPFWLSYPEMVRVAGGIPVFIHTTADQSFKITPQHIERL
                     IGPATKALVINSPSNPTGVMYTPEELRALGETALLHDITIISDEIYEKMVYDGQRQVS
                     MASFSKALYDNTITVNGFSKPYSMTGWRLGYTAGPEPFMKAMSALQSHCASAPNTFAQ
                     WGAITALEQGAASVAEMVAAFDARRRRICERMRAIPGVKCVIPTGAFYVFPDISSFGL
                     DSLTFSTRLLETEHMAVVPGAAFGADTCVRLSYACGMDEIEEGMTRFARFCASL"
     gene            complement(7480..>9095)
                     /locus_tag="PK576_04535"
     CDS             complement(7480..>9095)
                     /locus_tag="PK576_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60891.1"
                     /translation="AGTGTEIIGAGTCSIGNTAGETGDVFHVLDGAYWTNANACVIGY
                     AGYGNYGLISNATVYVTGGNLGAGSNGQYASNNVFEVKGGARVHVRGSNGKSLINHGR
                     WGYNNTLRIAGEGTFVDWWTNDHNDGSVVVGEMAMNNLLHVTDGARLGTNGCLGVGFV
                     GNVYYGNWRGDSIGNTLKVDGGAQCYMANLCVGGLCTGRMDPYDGTNGRNTYKRGPNG
                     EWWQNTYFDLSISSNAFIVSGSATRAQLAGSLQVAGKTNEGPCLAYVHENDFNRVRVE
                     DGAELTVGSVSLGKWGVSNHIEVVGGALLSVTTGNTGYYDGFSFGGFPTTGTWVRVSG
                     ANSRLSIPNSRLALMGCPDGRENKIEISDGALVTAKHVNSYATNNVFHLNNGTLTVTD
                     SLICPNGDANGAKPRFIFEGDAPRIRCTSTDDGCQFKTEPIFEFRIPEKGFADVPVKS
                     SGHLRIYGEPTLVLDGRCLRSYSAAGGGIQVLFEAAEGKTLTITSERIAKMSGEAAAV
                     LDGCAVTLVDKRKLVLSVPDRSGTLIRLH"
     assembly_gap    9096..9195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9196..9771)
                     /locus_tag="PK576_04540"
     CDS             complement(<9196..9771)
                     /locus_tag="PK576_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60892.1"
                     /translation="MKRMFCALVTGVLCAAGGVNAANTFTLWTGADGTDPTDLARPAN
                     WEGGAVPVIDTGTMDGPFGKFYSLGTVTVPLTLSEDLFLNGLVFDSVGTAFDLDLTGR
                     TLRLYGYSQDSHSLTFRTFRNVPETIRVRGGTLDMTDGKQKTVYVDGASDVSFSGTEL
                     RGGIQFRTLYGANVCLTNGVTGSAYFTTFAGT"
     gene            complement(9967..11010)
                     /locus_tag="PK576_04545"
     CDS             complement(9967..11010)
                     /locus_tag="PK576_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE60893.1"
                     /translation="MEEKVLVTGGSGFLGINLIRYLKGKGVTGIRSIDLEPFDYPEKA
                     EVDAVVGDIRDVEALRKVMEGVTWVVHTAAALPLYTEQDIMTTDVDGTRNVMTLAREA
                     GVRRAVMISSTAVYGIPDHHPLVETDRLIGVGPYGRAKILAEEVCLEARQQGLCVPII
                     RPKSFVGPERLGVFALLYDWAYTGHGFPMIGSGKNRYQLLDVEDLCEAIWLTLTRDEE
                     VVNDTFNIGAKIFGTMREDYQAVLDEAGYGKRIRGFPAAPMIWALRFLECLRLSPLYK
                     WVYETACEDSFVSIEKAERVLGYAPKFSNRDALVRNYRWYVANRETFKNASGVSHRVP
                     WKQGILRFAKIFF"
     gene            11165..11791
                     /locus_tag="PK576_04550"
     CDS             11165..11791
                     /locus_tag="PK576_04550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE60894.1"
                     /translation="MMHPPPSSGNPLPPVLRATPDCLSEAARLLCAGGIAVIPTDTVY
                     GIAAHPDRPEAVARLSAIKRRAEDKPIALLAADVGAVRAFGAHFPEGARRLADACWPG
                     ALTLVLACGERWEGFRVPDHAFTRALLAACGGTLRVTSANLSGAMPATSAAQALQDVG
                     LVADRVLDDGLSPGGTPSTVVKAEADGTLTVLREGAIPATAIRRLSRA"
     gene            11895..12719
                     /locus_tag="PK576_04555"
     CDS             11895..12719
                     /locus_tag="PK576_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE60895.1"
                     /translation="MGTFLLSVIIPTRNESRNLTACVESFSDAVAAGWCEVLVVDNNS
                     DDGTPQLAATLGARVFSQGPERSTQRNRGWREAVAPFVLFMDADMSLPPETLAEIHTC
                     LTSPQPPDALYIRERRTGGGWWTRVRNFERGFYNATCIDGLRVFRRALLETTGGYDET
                     LFACEDWDLDRRILALTENIALTNGFLLHHEERLTFRRHLRKKRYYSGSFSAYIKKWG
                     WDPVTRRQFGFWYRFVTVFLENGKWRQALRHPFLLLAVWAERVCVGLLCLRAVKKG"
     gene            complement(12706..13554)
                     /locus_tag="PK576_04560"
     CDS             complement(12706..13554)
                     /locus_tag="PK576_04560"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HOE60896.1"
                     /translation="MVGEETANLFEWQRNRLEVQRSAVFGAPQSSQVTVVTYHFWDVE
                     QADLRFDAVECALYETWRHCGKLKACVVVNRETPRLRAFAEAQGDAVTIRVNPALIPG
                     DLPSMSVDCIVNLHRYFDTDSVLVIQNDGFPLRGGIKAFEGRYDYIGAPFVRDTALNR
                     IMGLWPRFAVGNGGFSLRTKRICEMASFYWSKRWYHHMPRDWRFVREDAFYGVLLPWL
                     ETSYRRTIRFAPVDVALRFSYDDLYGACPAELPFGFHGSAAFREFVEKGLLKQGEQGA
                     AAVSPF"
     gene            13685..14695
                     /locus_tag="PK576_04565"
     CDS             13685..14695
                     /locus_tag="PK576_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE60897.1"
                     /translation="MRPDPNKAMEVTGTRRLTTDERTGLCVVPPMRPIHAILITYHPS
                     DRVLDTVRDAAEQADHVIVVDNSATESARNLLTQAFAPHTDRFTLIFNPVNLGVAAAL
                     NIGMRTAQVGKAHWVLTLDQDSRLTSGMVAALIAAWEAMPREQRSQIGVLAPQTVSAD
                     KTPSENTSAPIANWREVETAITSGCMICLEAWQAVGGFNEALFIDYVDHEFCFRLRWN
                     GWRVVQCPDAWLIHRIGNAEHRRLFGREITVNQHPPVRSYYIMRNGLYFWRAFPEPSA
                     FIRADKRNTLTLLLKALTVEPQRRERLSLFWRGYRDYRAGRFGAYSGPADVSPASEIH
                     PV"
     gene            complement(14620..15456)
                     /locus_tag="PK576_04570"
     CDS             complement(14620..15456)
                     /locus_tag="PK576_04570"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HOE60898.1"
                     /translation="MQTWQRTRLAAGQDERYVPSACTEVTALAYHFWDSERFDEQFAF
                     LECAIRETWRWCGLLKTVLVVNRVTPRIEAFAEAFSGWVRFDVLETLIPGNLHSMSAD
                     CNAHLHRRFDTPYVLVVQNDGFPIRSGLDAFVGVWDFIGAPYVRNTWCNRIVCGMLNC
                     WVSNGGFSLRSKRICELAAQYWRKKYCALPASRMVSEDVFFTETLPLRERAYRRAVRI
                     AAYPEAVRFSYDAAFPYTEPVPPFGFHGLKAFEFLRQTGWISDAGDTSAGPEYAPNRP
                     AR"
     gene            15618..16793
                     /locus_tag="PK576_04575"
     CDS             15618..16793
                     /locus_tag="PK576_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE60899.1"
                     /translation="MTTRICIIGGCGHVGIPLGLAFASRGFPVTLIDCNQTAVEQVSR
                     GSLPFKEEGAEELLHAHINKNLFATTDVSLAAQQDVIVFVTGTPVDEHLNPRVNDVLK
                     VVTSYLRFMNKSQLVILRSTIFPGVTQIVDHLLVQALGSSKLAFCPERIVQGKGIEEI
                     FNLPQIVSATSAQAEQEASELFSAIAKKIVPLSTAEAELAKLMTNAWRYLEFAIANQF
                     YMIVEKKGFDFYKIFNAMKDDYPRAKNFASAGLAAGPCLFKDTMQLSAFHSNEFFLGH
                     SAMLVNEGLPNFLVQQLEEKMGNLKGRKIGILGLAFKADNDDTRESLSFKVKKLLELQ
                     MAEVLLSDIYVAGTLPLQQFMQEAEGIILGVPHTEYRKLAPTCPYIDCWGVWPRPNS"
     gene            16820..17842
                     /locus_tag="PK576_04580"
     CDS             16820..17842
                     /locus_tag="PK576_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE60900.1"
                     /translation="MKILVTGSAGFICGYLVEELLNNGHEVVGIDNYSKYGKVEKSYD
                     NHPRYHFTEGDVKDLALMKNLIAGCDQVLGAAAMIGGISYFHELAYDLLAENERITAA
                     TFDAAIWAFKHSSLKKINILSSSMVFESATTFPSREEDVRKCPPPLSTYGFQKLACEY
                     FAQGAFEQYKLPYTIIRPFNCVGIGEKRALCDSEIVSGNIKLAMSHVVPDLVQKVVKG
                     QDPLHILGSGDQIRHYTYGGDLAHGIRLCIEKPEAINEDFNLSTPVSTSVLELAELIW
                     NKIHKGAKPFRYTSDTPFKYDVQKRVPSVEKAERLLGYKAETTLDQILDEVVPWIVEQ
                     VRIGGI"
     gene            17863..18591
                     /locus_tag="PK576_04585"
     CDS             17863..18591
                     /locus_tag="PK576_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE60901.1"
                     /translation="METQPELTIVIPVYNEGENILRSLREIQRHVTVPATVSIVYDFE
                     EDTTLAALKQLPAGSSLKVRAMRNAYGRGVLNAIKTGLEAADTELIIVTMADLSDPPE
                     VMNEMVRVARETGAAIVCASRYMRGGQQIGGPKLKGFLSRTAGMILYWVARLPTHDPT
                     NSFKLYRKSFLSTTTIESPGGFELGIELVVKAWKSGHLIAEVPTTWRDRVAGKSNFKL
                     WKWLPHYLRWFFYAFRRNKKHPPC"
     gene            18585..>18793
                     /locus_tag="PK576_04590"
     CDS             18585..>18793
                     /locus_tag="PK576_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60902.1"
                     /translation="MLIMPHPYGRMANRLILASTFIAQAEEYGDSFLHLAFADYCRFF
                     EGTRHRPFLYHRSTHPQPRVTKRLFG"
BASE COUNT         3839 a         5919 c         5287 g         3648 t
ORIGIN      
        1 cgcgggcgca tatatccatg attaacatga tgttgtccaa tcctgaaaat cctgtaatcc
       61 tcgcaattta taggcaagga tttaggagtt ttttcacttc gacgttctga gtccggcgtt
      121 gggcgttcgg cgttcaaagg cggcggcgtg aaacgccgcc cggacgagca gacgctcgtc
      181 cctcccgtcc tgcgggcaga acaaaaaatc tgcgaaatct gtgtaaatct gcggataaaa
      241 ccagaaagca caagacacgt cgttgcgggc ggggacgccc gcgcgcccga ctctccgctc
      301 cccttttcac tagaaacgca tgatcttcgc acatccctgc gtcggtcact aaccgattcc
      361 aagggttgcg gagcgttttc gcggtgtgct aatgtaccgc cgtggcaagg caactgaggc
      421 atcaccggaa gggtggagtc tcggctgaaa accaacaaga cttgtgtcgg tcgacggcgc
      481 acgcattgag ggtgctggaa aaatgaaatg tccaatgtca aactacgacc ccgataatca
      541 ttaaggaaag ctcccccgtc atgaaaaaac tgttatatat tgtttacgtt tttgtggccg
      601 ttctcacgct tacagtcgcg ttaagcatgt taaacgctaa gcatgaaaaa atgaacacgg
      661 tcgcaacggt gggcggggct gcgataagcg aaacgaacga cgccctgtcc atcatcgtga
      721 aaacactgct ggagcagttc gacaaagatg aaaacattgc ggtctcgccg caagagattg
      781 aaaaacaacg cgaattcttc gtgaagctgg ccaagatgat ttatgaccaa cggataacat
      841 accttaacaa agagttggaa atgctcaacg tttctggcga cggaactgta gacgtctcga
      901 acaaaaccga aaagatcagg aaagaaatgg catggctgga gcctagtttt ttcgtgcctg
      961 cagatgatgt attaagaacg aacgtacttc tatggaaacg gaacaaagca ctttataaac
     1021 gttatggcgg gaatgtctat ttttcgcatg ttggcccttt ccccgcagac gcttacaagg
     1081 cgtttttcaa agaaaaagag gccgaacgtg cttttgagat attcgatgaa aagctaagga
     1141 gcgatttctg gggcgagcaa ttcaactaca cgaaacaatc cgacctgttg ctagatgatg
     1201 gcaaaaaggc cattgagacg cctccctggg aagcgaccat cgacgggtga gcgacggacg
     1261 cggcaacccc cgcgccaccc gcgatcgtat tcccgggctt tactttacat aacggaaggt
     1321 tatattctct gttctacacc gtctgtgaac acgacgcgca caaggagaga ctatgatgag
     1381 ccgaacgaag accgcacttc ctcaccgcaa ggccgccacg cgccgcggct tcctgaaacg
     1441 caccctggcc gcctcgacct tgaccgcctt tcccgcgatc cgcgccacag gccggcccgt
     1501 gaccggcgac aaggtccgac tcgcctgctg cggcatcggc aatcaggggg ccagcgacat
     1561 caacaacctg tacgcgaccg gcctgtgcga catcgtggcc ttgtgcgaca ccgatatggg
     1621 cgcgccccac acgcggaaaa tcctcgatac gtttccggac gcgccgcggt tccaggattt
     1681 ccggaagatg ctggacaaac tgggcgggca gatcgacgcg ctgtgcatcg gcgtgccgga
     1741 tcacacgcat ttcccgatcg ccatgatggc gatggggctg ggcaagcacg tgtacgtcga
     1801 gaagccgatg gcccacacct tccgtgagat cgagttgatg atgaaggccg aaaaaaagaa
     1861 ccgggtcgcc gcgcagatgg gcaaccaagg ccactccggc gcgaactact tccagttcaa
     1921 ggcgtggacc gaggccggca tcatccggaa cgtgacgcgg atcgttgccc acatgaacgg
     1981 cgcgcggcgc tggcacaaat ggaacggcgt tgtcacaagc ctgcggccgg cccagcccgt
     2041 gcccgagacg ctggactggg acacctggct gggacaggtg cccttccacg gctatcaccg
     2101 cgattacgtg catggcgact ggcgctgctg gtacagcttc ggcaacggcg cgctcggcga
     2161 ctggggcgcg cacattctgg acacggccca ccagtttctg gaactgggga tgccgaccga
     2221 agtgagcgcc ctcaatctgg aaggccacaa cccctacgta ttcccgatgg cttccacgct
     2281 ctccttcaag ttcgcggcgc gcggcgcaat gccgccggtc gaagtcctgt ggtacgacgg
     2341 tgtgaagaac ttcccggaat tgccgccggg gttcggcggc tcggccaaaa ccgcggatga
     2401 catccccacc ccgggcggcg gctcgacggc ggtcagcaag ttgagtccgg gcaaggagat
     2461 ctacgccgaa gacctgacct tcaagggagg ctcgcacagc agcacgcttg agatcatccc
     2521 cgcggaaaag gcgcgcgaca tggccaaccg cctgccggaa tatccgaaaa accagtcgaa
     2581 ccactacaag aacttcctgc tggcgtgtat gggccgggaa acgtgccgtt cctcctttga
     2641 ggtcgcgggc cccctcagtc aggtgctggc gctggggtgc atcgcccagc gtctcaacac
     2701 cacgttcacc ctggatccgg tcacgcggcg gatcaccggc aacaaactcg ccaacgacct
     2761 gctggacggg ccgcccccgc gcaaagggtg ggaacagtac tatcgagtct gagccttttc
     2821 gggaccgtgc cccggcaacg cggacggtcc cgacccatca tgtcctcccc caacatttac
     2881 atccgaatcg gtacgcgccg tttccggctc gacaccgcag ccctcaagag gttggccgcc
     2941 gcacttgcga agcgggcgca gacgcgtttg cgaaacggcc ccgtctggcg ggagatcacg
     3001 atccacctgc tggacgacgc gggcatcaca ccggtgaacg ccgcgatcct gaagcatgcg
     3061 ggccccaccg atgtcatcac gcagcgctac gagccgatcc ccggcgaacc cgatggcctg
     3121 atcggcgaac tgttcgtgaa cgtcgaacgc gccgcgcgcg ccgcgccgcg ccgcgcaggc
     3181 tggaatgccg accgcgaact ggcgctctac ctcgcgcacg gcttcgatca cctggccggc
     3241 gcggaggacc gcacgcccgc agaacgggcg cgcatgcggc gccgcgagct ggcgtggctg
     3301 cgacaccttt cgttgacacc cctgttccga agcggtacga taccgattca aaaccgctcc
     3361 acacgggagt tttccatatg aatggcgcgt tattactgag tctctgcggg gttctttttc
     3421 tgctcgcgta caccatgtac gggcgcctca tcgaacgtat tctgggggtg gatcctgaac
     3481 gacccacccc cgcgcacaca ttgagagacg gcatcgatta cgtgccgacc catccggcgg
     3541 tcctcttcgg gcaccacttt gcgagcatcg cgggcgcagg gccgatcgtc gggccggtgt
     3601 tggcggcgca gttcggctgg gtggcggtcg cgctctgggt cgtgctgggc tgcatcttca
     3661 tcggcgcggt gcatgatctg gccgcgatat tcctttcgat ccgtcaccaa ggccgttcga
     3721 tcggtacggt gatcgagtcg ctgatgggat attgggggcg gatgctcttc ctctttttct
     3781 gctggttcac gctggttctg gttgtggcgg agtttctgcg tatcgtggcc gaaacgtttg
     3841 tcgagattcc ggcggtcggc acggcctcga tgctgttcat tgcgttggcg gtcggctttg
     3901 gccgtctggt ctaccggggc ggcatgagtg tcctgaccgg atcgctgatc tttgtgccgc
     3961 tgacctttgc ctgcgtctat ttcggcacgc tgatcccgct tgatctggtg aagctgctgg
     4021 gttgtgatgc ggcggtcgcc cggcagatct gggtggtggt gctgctggtc tactgcttcg
     4081 cggcctcggt gctgccggtg tggctgctgt tgcaaccgcg cgattacctc aattcctatc
     4141 tgctctacgc catgatggca ctcggtttcg ttggtattct ggtggcgcga ccggcgttga
     4201 atcttgacgc cttcaccggc tggcatgcgg tgagtccgtt gagcggccgc tccgatcctc
     4261 tgatcccgct gctgttcgtg actgtggcgt gcggcgcctg ctccggtttc cacgcgctgg
     4321 tggcctcggg cacgaccgcc aagcagatcg acaacgaacg ccacctccgg ccggttgctt
     4381 acggcggcat gctgatcgaa ggggtgctgg cgatcattgc gctggtcgcg gttggcgtga
     4441 tgacgcaagg cgagtacacg gtggcgctga aggagcaggg cgcggttaag ctgttcgctg
     4501 gcggcgtcgc gagcttcacc gacaaactgg ggttgccgca cgaggcgggc atcatcttta
     4561 tctcgctggc gctggcggct tttctcatga cgacgctcga taccgccacg cgcctggccc
     4621 gcttcacttg gcaagagctt ttcctgccgc gcgggcaatc gggagacgtg cctccggcta
     4681 ctgtcgcgcc gacgctaccc gcgtcccatg cgttcttctc taaccgctat atcgccacgc
     4741 tgattgcggt ggtcgccgca ggctggttgc tgctcggcgg cggggcgaag agcatctggc
     4801 cggtctttgc ctcgtccaat cagttgctcg ccgcgctgac gctgctcggt tgcagtctgt
     4861 ggctgttgcg ccacaagcgc ccgctcgccc tcacgctgtt gccgatgctc tttatgatgg
     4921 ccaccagcgg cgctgcgatc gttaccctct tcctgcgcaa tgcgcaggct tggcaggcgg
     4981 acggcttcaa ggccggaggc gtcatgacgt tgacgaccgg catcctgatc gtgatgtcgg
     5041 tcgcgttgat cgtgatgggg gctgtcagca tccggcagac catgaggcag agtgaaagga
     5101 aagtgtagac atggcgaagg aactggcgtt cgtcctcatc aatccgtatt cgatccggaa
     5161 atcgcgtacc ggcggcatca tcgcgcgtta tctcgtccgc accgatctca agctgatcgg
     5221 cgcgcgcgtc atcggtgcgt cggccgaact ggtcaatgcg ttcgccacct atctggagac
     5281 ctacgatccc gacgacccgg aaaagtgcgc gctccttgcg aactacgtac ggcgcttcca
     5341 catgccggac gccgcgaccg gacggccgca ccgttcaatc ctgctgctct tcgagggaga
     5401 cgacgcgatc cgcaagatct tcgatgtcac cggctcgatc cgccaacaat ggtgcagcgg
     5461 gcagagcgtg cgcgacacct acggcgacct tgtgcgcaac ccggcgaccg gcgaggtcga
     5521 gtattttgag ccggcggtgt tggtcggccc ctcccagaag gtcgtgggcg acatcctgcg
     5581 catgtggctg ccgcatctcg cgtcacaggc cggcatcgtg cgtggtgcgc tggacgtgcc
     5641 gggcgacgct gacgcgcagg agacgctggt gatgctcaag ccggacaact ggcgtcagcc
     5701 ttcgctgcgc gcgggcaaca tcatggacct gctctcgaac tccgggttgc gcatcgtggg
     5761 cgtgaagaag ttctccatgt cggtggcgca ggccgaggag ttttacgggc cggtgcgtga
     5821 ggggctcaag aaggttttca agcggtttgc ggcggatcgt gccgcgcagg cgttgtcgcg
     5881 cgaattcggc ttcccggtgg atgccgctcc gctcgatggt ctgtgcgaca tgctggcgcc
     5941 ctcctttgcc gagcgcgagt tcgagagtat cgtggagttc atgaccggac gtcgcccttc
     6001 gaactgtctg cccgaggaac gcaccctgcc cggcaccgag gagtgcctcg cgatcgtgta
     6061 cgagggtatt gacgccgtca gcaaaatccg cgatattctc ggtgcgacag accccctcaa
     6121 ggcgcgtccc ggctcgatcc gccgcgagtt cggcaccaac atcatggtca atgccgcgca
     6181 cgcgtcagat tccgtcgaga acgcccatcg cgagatgcgg atcatcgatg tggcccgcga
     6241 cccgcacttt gaagagctga ttcaaaacta ctacccatga aaaaactgaa cgacgcgatc
     6301 ctcgccatca aaccctccca aaccctcgcc atctccagtc tggccaaaca gatggccgaa
     6361 cagggatgcc acgtctgcaa cttctcggcc ggcgaaccgg acttcgatac gccggatctg
     6421 atcaaagaag ccgccatcac ggccctccgg cagggcaaga cgaagtacac gccggtcgcg
     6481 ggcgtgcccc aactctgcaa ggcgctttcc gccaaattcg cgcgcgacaa cggccttgac
     6541 tacaaaccct cgcagatcgt ggtgagctgc ggcggcaagc actcgctcgc gctggttttt
     6601 cagacgctgc tcaaccccgg tgacgaggtg ctgatcccca caccgttctg gctcagctac
     6661 ccggagatgg tccgcgtggc gggaggcata ccggttttca tccacaccac ggccgaccag
     6721 tcttttaaaa tcacgcccca gcacatcgag cgcttgatcg gccccgccac caaagccttg
     6781 gtgatcaaca gccccagtaa cccgaccggc gtgatgtaca cgcccgaaga gttgcgggct
     6841 ctgggcgaga ccgctctcct gcacgacatc acgatcatct cggacgagat ttacgagaag
     6901 atggtctacg acggccagcg ccaagtcagc atggcatcct tctccaaagc gctctacgac
     6961 aacaccatca cggtcaacgg cttcagcaag ccctactcca tgaccgggtg gcggctgggg
     7021 tatacggccg gtcctgaacc gttcatgaaa gccatgagcg cactgcagag ccattgcgcc
     7081 tccgccccga acaccttcgc gcagtggggc gcgatcacag ccctcgaaca gggcgcggcg
     7141 agcgttgccg aaatggtcgc cgcctttgac gcccgccgcc gccgcatctg cgaacgcatg
     7201 cgcgccatcc cgggcgtcaa gtgcgtcatc cccacgggcg ccttctacgt cttcccggac
     7261 atctcgtcgt tcggcctcga ctctttgacc ttctcgacgc gcctgctcga gacggaacac
     7321 atggcggtcg ttccgggcgc ggcctttggt gccgacacct gcgtgcgcct tagctacgcc
     7381 tgcggcatgg acgaaatcga ggaggggatg acccgcttcg cccgcttctg tgcctcgctg
     7441 tgacagtcac gacggtcgtg acgtgaaagg atcgtcgggc tagtgaagcc ggattaacgt
     7501 gccgcttcta tccgggacgc ttagcacgag cttgcgtttg tccacgagcg tcacggcaca
     7561 gccatcgagc acggccgcgg cctccccgct catcttcgcg atccgctcgg acgtgatggt
     7621 gagcgtcttt ccttcagcgg cctcaaacag aacctggatg ccgccacccg cagccgaata
     7681 ggagcggagg caccggccgt cgagcacaag cgtgggctcg ccgtagatgc gcagatgtcc
     7741 cgaggacttg accggaacat ccgcaaaccc cttctccggg atcctgaact caaagatcgg
     7801 ctccgtcttg aactggcagc cgtcatctgt ggaggtgcag cgtatccgcg gcgcgtcgcc
     7861 ttcaaagatg aaacggggct tcgcgccgtt cgcatcgccg ttcggacaga tcagcgagtc
     7921 cgtcaccgta agcgttccgt tgttcagatg gaaaacgttg ttcgtcgcgt aagagttgac
     7981 gtgtttcgcc gtcacgagcg cgccgtccga aatctcgatc ttgttctccc ggccgtccgg
     8041 acagcccatc agggcaagtc tggaattcgg aatggaaagc cgcgaattcg cgccggaaac
     8101 gcgcacccac gttccggtgg tgggaaaacc gccgaacgag aagccgtcat aataacccgt
     8161 attccccgtg gtgacgctga ggagcgcacc accgaccacc tcgatgtggt tcgagacccc
     8221 ccacttgccc aggctcacgg atccgacggt cagctcggcg ccgtcctcga cgcgtacacg
     8281 gttgaaatcg ttctcgtgca cataggcgag gcaaggtccc tcgttcgttt tccccgccac
     8341 ctgaagcgag cccgccagtt gcgcacgggt cgcgctgccc gaaacgatga acgcgttcga
     8401 ggagatcgaa aggtcgaaat aggtgttctg ccaccactcg ccgttcggcc cgcgtttgta
     8461 ggtgttacgg ccgtttgtac catcgtaggg atccatccgt ccggtgcaga gtccgcccac
     8521 gcagaggtta gccatgtaac attgagcccc gccgtccacc ttgagcgtgt tcccgatcga
     8581 atcgccgcgc caattgccgt aatagacgtt ccccacgaaa ccgacgccga ggcacccgtt
     8641 cgttccgaga cgcgcgccgt ccgtgacgtg gagaaggttg ttcatggcca tttcacccac
     8701 gacaaccgaa ccatcattgt gatcgtttgt ccaccagtcg acgaaggtcc cttcacccgc
     8761 gatgcggagc gtgttgttgt acccccaccg gccgtggttg ataagggact taccgttgga
     8821 gccccttacg tgcacacgcg ccccgccctt cacctcaaaa acgttattcg aggcgtactg
     8881 accgttactg cccgcgccca gattgccgcc cgtgacgtac accgtcgcat tggagataag
     8941 cccgtaattg ccgtacccgg catatccgat cacgcaggcg ttggcgttcg tccaatacgc
     9001 gccgtcgaga acgtggaaca cgtcgcccgt ctcgcccgcc gtgttgccga tcgagcatgt
     9061 gcccgcgccg atgatttccg tgcccgtgcc cgcgannnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnncgtgc ccgcgaacgt cgtgaaatac gcggaacccg tgacgccgtt
     9241 cgtgaggcag acgttcgcgc cgtaaagcgt acggaactgg atgccccccc gcagttccgt
     9301 accggaaaac gaaacgtcgc tcgcaccgtc gacgtacacg gtcttctgtt tgccgtccgt
     9361 catatcgagc gtgccgcccc gcacgcggat cgtctccgga acgttgcgaa atgtgcgaaa
     9421 cgtgagggaa tgcgagtcct gcgagtagcc gtagagccgg agtgtccgcc ccgtcagatc
     9481 gagatcgaag gcggtcccga cggaatcgaa gacgagaccg ttcaagaaga gatcctcgct
     9541 caacgtgagc gggacggtca ctgtgcccag cgaatagaac tttccgaacg ggccgtccat
     9601 cgttcccgtg tcgatcaccg gcacggcgcc gccctcccag ttcgccggtc gggcgaggtc
     9661 cgtcggatcc gtcccgtcgg ccccggtcca caaggtgaac gtgttcgccg cattcacgcc
     9721 gcccgccgcg cacaaaacac ccgtaacaag cgcacaaaac attcgtttca tatcacgacc
     9781 ttcctgtcct gttaaatacc agagcttacg attgaatgac attatgcggg ggcaggcgaa
     9841 aagagtcaac cccggaaaac cgcagcacgt ttttggcgaa caggagccgt gcgggaaccg
     9901 tccgtttagc cgccggctcg ggagtcctta ctcaactcac gggcaagcag acacttgccg
     9961 ctcccatcag aaaaaaatct tcgcgaaacg cagaatcccc tgcttccacg gcacgcggtg
    10021 cgaaacccct gacgcatttt tgaaagtctc ccggttcgcc acataccagc ggtaattccg
    10081 caccagcgcg tcccggttcg aaaatttcgg cgcgtatccc agaacacgtt cggccttttc
    10141 aatgctcacg aacgaatctt cgcatgccgt ctcgtacacc cacttgtaca ggggagacag
    10201 gcgcaggcat tccaaaaagc gcagggccca gatcatcggc gcggcaggga acccgcggat
    10261 ccgtttaccg taacccgcct catccagcac ggcctggtaa tcctcgcgca tggttccgaa
    10321 gattttggcg ccgatgttga acgtgtcgtt caccacctcc tcgtcccggg tcagcgtcag
    10381 ccagatcgcc tcgcacagat cctccacatc gagcaactga tagcggttct tgccgctgcc
    10441 gatcatcggg aacccgtgtc cggtataggc ccagtcatag agcagcgcga acacgccgag
    10501 ccgttccgga ccgacaaagg atttgggccg gatgatcggc acgcaaaggc cctgctgacg
    10561 ggcttcgagg catacctcct cagccagaat cttggcacgg ccataggggc caacgccgat
    10621 cagccggtcg gtttccacaa gcggatggtg atcgggtatc ccgtacacgg cggtcgagga
    10681 gatcatgacg gcgcgccgga ccccggcctc ccgggccaga gtcatgacat tccgtgtccc
    10741 gtccacatcc gtggtcatga tgtcctgctc ggtgtacagc ggcaacgccg cagccgtatg
    10801 cacgacccac gtgaccccct ccatcacctt ccgcaacgcc tcgacatccc ggatgtcgcc
    10861 caccacggca tccacttccg ccttctccgg gtaatcgaac ggctcgagat cgatcgaacg
    10921 gatcccggtg acccccttac ctttcaaata acggatcaga ttgattccca gaaagccgct
    10981 gccccctgtc accaacactt tctcttccat atcaaccttc ctctgaaacg cgcttctccc
    11041 gcatgtctcc gatgatcagc cgaccgcaaa acacatgata ccccccttgc tcttccggtt
    11101 caagtatgat aaatcaaatc cggcatcccg atccgctcct gcgcctgaac cgtgtctgaa
    11161 caacatgatg cacccacccc cctcttccgg aaaccccctc cctcctgtcc tgcgcgccac
    11221 gcccgattgc ctgtccgagg cggcgcgcct cctgtgcgcc ggcgggatcg ccgtgatccc
    11281 gaccgacacc gtatacggga ttgcggccca tccggatcgc ccggaagcgg tggcacggct
    11341 ttcggccatt aaaaggcggg cggaagacaa gcctatcgcc ttgctcgccg cggatgtcgg
    11401 cgcggtgcgc gcgttcggcg cccatttccc ggaaggggcc cggcgtctgg cggacgcctg
    11461 ctggcccggc gcgctgacgc tggttctggc gtgcggcgaa cgctgggagg gattccgggt
    11521 tccggaccac gccttcacac gcgcgctgct ggctgcgtgc ggcggcaccc tgcgcgtcac
    11581 cagtgccaac ctcagcggcg caatgcccgc gacgagcgcc gcccaggcgc ttcaggatgt
    11641 ggggcttgtg gcggatcggg tactggatga cggcctctcg ccgggcggca ctcccagcac
    11701 ggtggtgaaa gcagaggcgg acggcacact caccgtgctg cgtgagggcg cgatcccggc
    11761 aaccgccatc cggcgtctct cacgcgccta agcccggcca accggacccg cacgccccgt
    11821 aaatcaatct tgttttccgg tatgattctg ccatcatacg tattacgaaa aaagagaggg
    11881 cttggttacg cgttatgggt acatttctgc taagtgtcat catcccgacc cgcaacgaat
    11941 cgcggaatct caccgcgtgc gtcgaatcgt tttccgacgc tgttgccgca ggttggtgcg
    12001 aagtccttgt ggtcgataat aactccgatg acggaacgcc gcaactcgcc gccacactcg
    12061 gtgcccgggt gttcagccaa ggccccgagc gcagcacgca gcggaatcga ggctggcgcg
    12121 aggcagtggc cccgtttgtg ttgttcatgg atgccgacat gtcccttccg ccggagaccc
    12181 tggcggaaat ccacacgtgc ctcacctctc cgcaaccgcc cgacgccctc tacatccggg
    12241 aacgccgcac cggcggaggc tggtggactc gcgtgcggaa tttcgaacgc gggttttaca
    12301 acgcgacctg cattgacggc ctgcgcgtgt tccggcgtgc cctgctggaa acaacaggcg
    12361 ggtatgacga aaccctgttc gcctgcgagg attgggatct ggaccgccgg attctggccc
    12421 tcaccgaaaa catcgccctg acaaacggat ttctgcttca ccacgaggaa cggctgacct
    12481 tccgacgcca tctccgcaag aaacggtact attccggatc cttctccgcc tacatcaaaa
    12541 agtggggttg ggacccggtg acccggcggc agtttggatt ctggtatcgc tttgtcaccg
    12601 tgtttttgga gaacggcaaa tggcggcagg ccctgcggca cccgttcctc ctgttggcgg
    12661 tctgggccga gcgcgtatgc gtggggctgc tctgcctgcg cgccgtcaaa aagggctgac
    12721 cgccgccgct ccctgttcgc cctgcttcag cagccccttc tccacaaact cccgaaaggc
    12781 agccgaaccg tgaaatccga acggcagttc agccgggcat gcgccataca ggtcgtcgta
    12841 agagaaccgc agcgcaacgt ccaccggtgc gaaccggatc gtccgccgat atgacgtctc
    12901 caaccatggc agcaggacac cgtaaaaggc gtcctccctc acaaaccgcc aatcccgcgg
    12961 catgtggtga taccaccgct tcgaccagta gaaggaggcc atctcgcaga ttcgtttcgt
    13021 tcgtagtgag aaccccccat tgcccacggc aaatcgcggc cacagcccca ttatccgatt
    13081 aagggccgtg tcgcgtacaa agggcgcgcc gatgtagtcg taacggcctt cgaatgcctt
    13141 gatcccgccc cgcaggggaa aaccgtcatt ctggatgacc agaacggaat ccgtatcaaa
    13201 ataccggtgc aggttgacaa tgcaatccac actcatcgaa ggaagatcgc ccggaatcag
    13261 cgcgggattg acccggatcg tcaccgcatc tccctgtgcc tccgcaaagg cccgcaggcg
    13321 cggggtctcc cggttgacga ccacacacgc ctttagtttc ccgcaatgcc gccacgtttc
    13381 gtacagcgcg cactccacag catcaaaacg caggtcagcc tgttccacat cccaaaagtg
    13441 atatgtcacc accgtaacct gagatgactg cggcgctcca aagacggccg accgctgcac
    13501 ttcgagccga ttacgctgcc attcgaacag gtttgccgtt tcctcgccca ccacagcacc
    13561 gctccaattg aaaccccctc ctcccgatca cgttagacta taccatagcc gaaaaacgct
    13621 gtcatccccg taagagacat gttatggtat attgatattg atgttaggtt tgaattcaat
    13681 tcggatgaga ccagacccca acaaagcgat ggaggtgacg ggaacccgcc gtctcaccac
    13741 tgacgaacga accggcctct gcgtcgttcc gcctatgcgc ccaatccacg ccatcctcat
    13801 cacctatcac ccttccgacc gtgtgctcga cactgtgcgg gatgcggcag agcaggcaga
    13861 tcatgtaatt gtggtcgata acagtgcaac tgagtctgcg cgtaaccttc tgactcaagc
    13921 cttcgccccg cataccgacc ggttcaccct gattttcaac cccgttaatc tgggtgtcgc
    13981 cgcagccttg aatatcggca tgcgcaccgc ccaagtcggc aaggcacatt gggtcctgac
    14041 gctcgatcag gatagccgcc tgacatccgg aatggttgcc gcgctgattg ccgcatggga
    14101 ggcaatgccg cgagaacagc ggtcgcaaat cggtgttctc gcgccgcaga ccgtgtctgc
    14161 ggacaaaaca ccctccgaaa acacgtccgc accgatcgca aactggcgtg aggtggagac
    14221 cgccatcacc tcgggatgca tgatttgtct ggaggcctgg caggcggtcg gagggtttaa
    14281 cgaagcactg ttcatcgatt acgtggatca cgaattctgt ttccgactgc ggtggaacgg
    14341 gtggcgggtc gtccagtgtc cggacgcgtg gctgattcac aggatcggca acgccgaaca
    14401 ccgccgcctt ttcgggaggg agatcaccgt gaaccagcac cccccggtcc gctcatacta
    14461 catcatgcgt aacgggttgt atttctggcg cgcctttccc gaaccgtccg cgttcatccg
    14521 tgcggacaag cgcaacaccc tgaccctact cctgaaagcg ctgacggtcg aaccccaaag
    14581 gcgggaacgg ctgagcctgt tctggcgcgg ataccgggat taccgcgctg gacggttcgg
    14641 cgcatattcc gggccggcag acgtgtcccc cgcatcggag atccacccgg tctgacgcag
    14701 aaattcaaag gctttcagac cgtgaaaccc gaagggcggc acgggttcag tgtacgggaa
    14761 ggcggcatca taggagaagc ggaccgcctc gggatacgct gcgatgcgta cggcacgccg
    14821 gtaggcgcgt tcccgcaacg gtagcgtttc cgtgaaaaaa acgtcctccg aaaccattcg
    14881 ggaggccggc agcgcacagt actttttccg ccagtactgg gcggccaact cgcagatccg
    14941 cttcgaacgc aaggagaacc caccattact aacccagcag ttcagcatcc cgcagacgat
    15001 gcgattacac caggtgttgc gcacataggg tgcgccgata aaatcccata ccccgacaaa
    15061 cgcatccagt ccgctccgga tcgggaaccc gtcattttgt accaccagaa catacggcgt
    15121 gtcgaaccgc cggtgcagat gcgcattaca atccgcactc atggaatgga gattgccggg
    15181 gatcagcgtt tccaacacgt caaaccggac ccatccggag aacgcctccg cgaacgcctc
    15241 aatccggggg gtcacccggt tcaccaccag caccgtcttc agcaagccgc accaccgcca
    15301 cgtctcccgg atcgcgcatt cgagaaacgc gaactgctcg tcgaaccgtt cgctgtccca
    15361 gaaatgatag gccagcgccg tgacctccgt gcacgccgaa ggcacatatc gctcgtcctg
    15421 cccagcggcc aaacgcgtcc gctgccatgt ttgcatccca ttcatattct tcgaaaaacg
    15481 tccgcacccg ccttccgggc ctctccgccc ctgaaggcca cagtccgcct accctcatga
    15541 aaaggaaagg cgtggattca caatacacca aataacccgg atagacaacc taacccggtt
    15601 gaagtaatat accccatatg acaacgcgta tatgtatcat tggcggatgt ggccatgtcg
    15661 gtattccgct cgggctggca tttgcaagcc ggggttttcc ggtcacgtta attgactgca
    15721 atcaaacagc ggtcgaacag gtgtcaagag gaagcctgcc tttcaaagag gaaggggccg
    15781 aagagttgct gcatgcccat atcaacaaaa atctttttgc cactaccgac gtttcgcttg
    15841 cagcccaaca agatgtcatc gtttttgtca ccggaactcc ggtggacgaa cacttgaatc
    15901 cgcgtgtcaa tgacgtgctc aaagttgtca catcctacct tcgtttcatg aacaaaagcc
    15961 agcttgtcat cttgcgaagc accatcttcc ctggcgttac ccaaatcgtc gatcatctgc
    16021 ttgtgcaggc actcggcagt tctaagttag ctttttgtcc ggaacgcatc gtccaaggca
    16081 aagggattga ggaaatcttc aacctccctc aaatcgtttc ggccacctca gcacaggctg
    16141 agcaagaagc ctctgaactc ttttcagcca tcgctaaaaa aattgttccg ctctccacgg
    16201 ccgaggcgga actggcgaaa ctcatgacga acgcctggcg ttatctggaa ttcgccatcg
    16261 ccaaccaatt ctacatgatc gttgaaaaga aggggttcga cttctacaag atctttaacg
    16321 ccatgaagga tgactatccc cgcgcaaaaa attttgcgtc cgccgggctc gctgcaggcc
    16381 cctgcttatt caaggatacg atgcaactct cggcctttca cagcaatgag ttttttctcg
    16441 gacactccgc catgctggtc aacgaagggc tgccgaactt ccttgtgcaa cagttggagg
    16501 agaaaatggg caacctgaag gggcgcaaaa tcggtatcct cggactcgcc ttcaaagctg
    16561 ataatgacga cactcgcgag agtctgtcat tcaaagtgaa aaagctgttg gaactgcaaa
    16621 tggcggaggt tctcctgtcg gatatctacg tcgccggaac attgccactt caacaattta
    16681 tgcaggaagc cgaaggaatc atccttggcg ttccccacac ggaatatcgg aagcttgccc
    16741 ccacatgccc ctatatagac tgttgggggg tgtggccaag accaaacagt taaaccactt
    16801 cctaaggacg agacacgtta tgaaaattct tgttactggc tcagcgggtt ttatttgcgg
    16861 gtaccttgtt gaagaattgc tcaacaacgg gcatgaggtt gtcgggatcg acaattactc
    16921 aaaatatggc aaagttgaga agtcctatga caatcatccc cgttatcatt ttacggaagg
    16981 ggatgttaaa gacttggcgc tgatgaaaaa cctgatcgcg ggctgtgatc aagttttggg
    17041 agcagcggca atgatcggcg gcatctccta tttccacgag ctggcctatg acctcctcgc
    17101 cgagaacgag cgcatcacgg ctgccacgtt cgacgccgca atttgggcct tcaagcacag
    17161 ctcgctcaag aagatcaata ttctcagttc aagcatggtg ttcgagtcgg ctacgacttt
    17221 cccgagccgc gaggaggacg tgcgtaagtg cccgcccccc ttaagcactt acgggttcca
    17281 gaaactggcc tgcgagtatt tcgcgcaagg tgcctttgag cagtacaagc tgccttacac
    17341 catcatccgc cccttcaatt gcgtcggcat cggcgagaaa cgcgcactgt gtgacagcga
    17401 gattgtctct ggcaacatca aactggccat gagccacgtc gtgcctgatc tcgtccagaa
    17461 agtggtgaaa ggccaagatc cgttgcacat tctcggatct ggcgatcaaa tccgtcacta
    17521 cacctacggc ggtgacctcg cccacggcat ccgcctctgc atcgaaaaac cggaggcgat
    17581 caacgaagac ttcaacctct ccactcccgt ctccacctcc gtgctcgaac ttgcggaact
    17641 gatctggaac aagatccaca agggtgcaaa gccgttccgc tacacctcgg acacgccgtt
    17701 caagtacgat gtgcagaagc gagtgccatc cgttgagaag gcggagcgcc tattaggtta
    17761 caaggctgaa actacgctgg accagatcct tgacgaggtc gtcccctgga tcgtcgagca
    17821 agtccgcatc ggtggtatct gaggagcaca tcgcaagttt ttatggaaac gcagcccgag
    17881 ttgacaatcg tcatccccgt ctacaacgag ggggagaaca ttctgcggtc gctgcgagag
    17941 attcaacggc atgtcaccgt gccagcgacg gtcagtatcg tttacgactt cgaggaagac
    18001 accacactgg cggcgctcaa gcagttgcca gcaggatcgt ccttaaaggt tcgggccatg
    18061 cgaaacgcct acggacgcgg cgttctcaac gccatcaaga ccggacttga agctgcggac
    18121 accgaactga tcatcgtcac catggccgac ctttctgacc cgcccgaagt gatgaacgag
    18181 atggttcgtg tggcgcggga aactggcgcc gccatcgtct gtgcctcgcg ctacatgcgc
    18241 ggcggacaac agatcggcgg ccccaagctc aaaggctttc tgtcccgaac cgcagggatg
    18301 atcctctact gggtcgcacg gcttccgacg cacgacccga ccaacagctt caagctgtac
    18361 cgcaagtcgt ttctttccac caccaccatc gaaagcccgg gcggattcga actgggcatc
    18421 gaattggtcg tgaaagcgtg gaagagcggt cacctcatcg cggaggtccc gaccacttgg
    18481 cgtgaccggg tggcaggcaa atccaacttc aagctctgga agtggctgcc tcactacctg
    18541 cggtggtttt tttacgcatt ccggagaaac aaaaaacatc cgccatgctg atcatgcccc
    18601 atccctacgg ccgcatggcc aaccgcctga tcctggcctc cacattcatc gcgcaggccg
    18661 aggagtacgg ggattcattt ctgcatctgg cgttcgccga ctactgccgt ttctttgaag
    18721 gcacgcggca ccgccccttc ctctaccacc gttcgacaca cccccagccc cgcgtcacga
    18781 aacgcctgtt cgg
//
LOCUS       DAOSLE010000043        18488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3229_length_18488_cov_8.308035, whole genome shotgun sequence.
ACCESSION   DAOSLE010000043 DAOSLE010000000
VERSION     DAOSLE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18488
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3229_length_18488_cov_8.308035"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..3237)
                     /locus_tag="PK576_04595"
     CDS             complement(181..3237)
                     /locus_tag="PK576_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60903.1"
                     /translation="MRAGRFRRGGWTALLMGGPVWAFCAAGDIVRPMGSFDRAEFTRP
                     EDAERVRSSGNLAGTSEPGKNCYIKGWAEYDIEVPQSDWYELIVPGQAWGVEYRFDGD
                     VAVSAGSGKIGNFWLEAGRRTLRVQRYHWTGFQRIESFTLRPAEKKPAKRLRLWMPGE
                     NRIVRTGEVLPLRLQAGGPDGQPGWVTILLSDPKTQEIISQAEVAVPVGERPAEQPVT
                     LACPREGLFELRVSADGRRIDNLDVPVRPVVVVDTRPAARGGELRKALIAEIDCAVAA
                     PQFSGTGGANVVRSEAGVYRESGDAGFLRAQHNKQECGWFAYRLGVPEAQAPYVLEAD
                     YPDDAFRTFCIAIREAAPDAYPIAGGVDSGECYALTRRMQTHALLFWPRTRDLRVLFV
                     TAHNGRRGAAVSKIRLYRVEGDLPLPSVPPRGGRSFGNWYEEGSNFMAVYGAPDRSPR
                     GFLIGADRWARSIASVGGDTLVSTVAVYQMAMYPSRYNIDFSEPHSFDVVRVLLLKCE
                     KYGLGFIGEFHPECRELDRLADPTGQITPKPNGLISKDGKGGKDWSAIPRFHPLHPVD
                     REWYLGMIGEFVDRYKDSPAFRGVSLRLMGWANPGLNNFHSLDWGYDDLTVSLFEKET
                     GLSTGARADDPKRFRARYDWLMANARGAWTAWRCRKIMELHAAIRDRVRRARPDLTVY
                     LNNFGFGEEAGIAPKLLHALDGVVLVNAGHAYGRRAYTYEGPLADSKRRDKLLDPAAL
                     LALRNTEGKTAFLFGAGYFEATERVVPPASLGFPEDTKRTWMSGVVNPAGRHYLERYA
                     LALAEADAQYLSDGGNAYTLGQPLLTDFLREYRALPALPFRPREDGRDPVAVWELPGQ
                     ERFLFYAVNRERFPVPVAIRLTGTSEVRRFSSAKPQRLEDGVLRLTLQPYELAAFEAG
                     GGAALAGVTEQVPPEELARAEKQTAWMEALAVEARADRVGGQLSEAQAAQVVEAAAAS
                     RAALACRHVWQARTLLERSGLIQVYRILKRFPPALYDMGDESAGVR"
     gene            complement(3261..4535)
                     /locus_tag="PK576_04600"
     CDS             complement(3261..4535)
                     /locus_tag="PK576_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOE60904.1"
                     /translation="MRRWIGCLVMAALTARGAGQDGVFQVTAYGAVPDGVTDNTAAIQ
                     KAIDACAAAGGGTVRVAGGAFMTYTLYPRSNTRLEIEAGASLEGGPDPLRYPEFVPSP
                     HWRVEYSLRRNKRAMFYAVAQTNLTICGRGTINGHAELFTEPSGSGHWWKRKHDQLIT
                     GRNIFLVGCSDVTLEGFSIYNPAGWSMWLLDCDRVNIRGLKIDMNLKFPNGDGIHIGS
                     CRDVTVSDCIVRSSDDSIILRAYQHQLNEPKACERVVVANCVLESRSSAIRIGWTHDY
                     EIKDCRFSNLVIRDSITGISIVMPAVAHVPNDPPRGPGVPPLPEGDAVRPFGVENVHF
                     SDISMNCRDPIRVSFAPDTKVSRLRNITFSNMTIRSPEYPSFVLRKGDRVSDILLSNV
                     RFELLPGGKNTFNIRGVERLTLDRVTFIDSSE"
     gene            complement(4621..5499)
                     /locus_tag="PK576_04605"
     CDS             complement(4621..5499)
                     /locus_tag="PK576_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOE60905.1"
                     /translation="MMKAGRILSVAFWVATGAVLADEPVCPLGSDWVLDATVSDEFNG
                     TGIDKAKWWDYAPRWRGRREFLYSHKNVEVRDGFLSLYARNVNPEEMAYEDREDGILP
                     YTCAIIKSRKKVTYGYFECRARGNAAEVRNAFWLYDPLSDRLSKKVRPGSHSEEIDIY
                     EFIGKYQDPSCLPYEICAHVHRFETPYVEGLVNHVKTKLANPGGAVAVDWAPCDGYHL
                     YGFLWTEKEMVWYVDGKEYFRRDNDYFHTPLHVMFDCEIATWRRASVKRLDPATLPAS
                     HSVDWFRRWVKRVPGR"
     gene            complement(5644..5964)
                     /locus_tag="PK576_04610"
     CDS             complement(5644..5964)
                     /locus_tag="PK576_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60906.1"
                     /translation="MRRFPLVPAMICVLLGIGDGMARVRVEETTVTLPTYPPGPYDKN
                     PIFYTGRVYQGAQGRVYPYPLQDTLHDEKVDQTYASLRLENECLEMTLLPNGSPTVRP
                     WRFI"
     gene            6277..7161
                     /locus_tag="PK576_04615"
     CDS             6277..7161
                     /locus_tag="PK576_04615"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOE60907.1"
                     /translation="MFFFDKIRRGEPTISFEFFPPKTPDGWARLYATIAETARLNVDF
                     VSVTYGAGGSTRESTVELVASLQNNLAIHAMAHLTCLGHSQAELARILDRLAEKRVPA
                     IMALRGDPPQGETAFRPHPYGFAHADDLIGFIRDGWPDFKIGCAAYPEGHPESFAPGE
                     ARTPDIDIGILRRKQENGADFAVTQLFFDNAVYFHFVDQARAAGVTIPLLPGIMPMSS
                     AKQIERFIALAGCSIPETLKQAARAPDVAEAGFLFALDQCRDLLTRGAPGIHLYSLNQ
                     AELSSRLLTALRDERLLR"
     gene            7203..9023
                     /gene="mutL"
                     /locus_tag="PK576_04620"
     CDS             7203..9023
                     /gene="mutL"
                     /locus_tag="PK576_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE60908.1"
                     /translation="MVKDGHIRLLPIHIANKIAAGEVVERPASVVKELIENAIDAGAT
                     RLDITVTAGGRKLIAVSDNGCGMNRDDALLSIERQATSKITDVDDIERIDTLGFRGEA
                     LPSIASVSRFLLKTRRAEKDAGTELTVVGGSLQDVREAGLPPGTTIEVRDLFFNVPAR
                     RKFLRAYQTEQAHIRSVFTLHALAHPEIGFSLAADGRELARLPCDATHEERIRDLFGA
                     AFCAGLRPVDRSVRGVRVTGFAGLPNLTRTDRSEQYVFINRRPATAPVIAYALREAYP
                     PLEGNRKPIVLLFIELPPDEVDVNVHPTKREVRFRRPADVREAIILAVRAALGRKRET
                     RAAAPAGHQDEAAVTLRALVPEAAAQTRGEPFPYPGPVALPPTDAPAAAPPSPAKSLP
                     TEPPPAPTGSGLPLFHTPEDGSPWRWCRVMGQVGGRYLLLETDGGYVTLDPCAARERV
                     LYERLMTVHRSGGVVSQALLLPETVRLPPADAARLRKFLTVVQEMGFGIADFGSDHFM
                     VESLPDALAGVSCRELLGNIAQDLENVGTRRGADKWRQELVAKAACRAAAGGMAPLSD
                     DAAEKLVADLAETQMPYTCPRGRPTMIFTSYRELERKFGR"
     gene            9181..10869
                     /locus_tag="PK576_04625"
     CDS             9181..10869
                     /locus_tag="PK576_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60909.1"
                     /translation="MACRVLCAADADAQPAARVKAVVRILPQTVDVRNDAFLIGLTAG
                     CDGGAARAVRAERLPHEIPEAELRSSCPTGGAVRISVSHVPGETAPFYTVVNNSDTEQ
                     GVTLLAQALRQGVPHTLSVICRSERDKTRLTVGFQSAADPAAPETFRRARIPSGSKWN
                     RHTLTFTPDRDGTFRLRFLLEPGGIVTFARFSLMPADADENGWDPVALEALRVTAAPF
                     LFWPVCSGSGFYNWYDGVGPQAAREAKGQAFGTVEAVRLSRLSGAEPVFRISLFAPED
                     ASEATGIPRPAAAAELAADWVAYCNATGSHPLAMLRTRHGVTEPLGVRRWELCPAGGL
                     LRDPSAFARESRRVADAMRAEDATLTLQIRPALTNAYPVSDPYTGRLLKRLESADAAE
                     REYYRAWYEALGPLSARLDLRAAESDRDNTAPLAFTLPDVLCRNVSPPACLTGPGLLM
                     TLLNRFPLAGILATEGAPPTVRILAGWGEDDSVLLLFVCNTGTEAQTLHFDLRALRRR
                     FMFYTLDQAAAEITLPRLTPVLPIRRHQKAGAALTQFVVCEAAPSSFTRILVTE"
     gene            complement(10897..11763)
                     /gene="lsrF"
                     /locus_tag="PK576_04630"
     CDS             complement(10897..11763)
                     /gene="lsrF"
                     /locus_tag="PK576_04630"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HOE60910.1"
                     /translation="MADLDDIRDGKEYGVGTAQKTEGFFLKGSSHLDWGMKDRLSRIF
                     NPATGRTVMLAFDHGFIMGPTSGVERIDLSIVPLIEYADCLMCARGVLRSLVPPTCRK
                     PICLRSDAGTSILTELNHNVVIDVQDAVRLNASAIAVMVAVGDAATEAETVANLYQAV
                     DAGQACGIPVMGVTAVGKDMARDARYFGLASRLCAENGANIVKTYYTEGFEKVVACCP
                     VPIVIAGGKKLPELEALELAYRAIDSGAAGVDMGRNVFQSEKPAAMIQAVSAVVQQGL
                     KPKDAFQMFNDL"
     gene            complement(11791..12438)
                     /locus_tag="PK576_04635"
     CDS             complement(11791..12438)
                     /locus_tag="PK576_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOE60911.1"
                     /translation="MTTSGETNSMLTLARFKMMIAAAGDAIRAQEKRFSELDAAAGGD
                     GDHGTAIVTAFNAMSQADGADFRSYLKALSDALQNEACGSTSTLYGAWLQGMGEAAPE
                     GAAALDEAALAAVFQGGLEEIGFTTRARVGDKTLMDVLIPATGALVAAQGGGVPAMLA
                     KAAEAADRGAESTCALRAKFGRAKNLGDRSIGPCDAGAASMACIFAAFAKAAETK"
     gene            complement(12435..13427)
                     /locus_tag="PK576_04640"
     CDS             complement(12435..13427)
                     /locus_tag="PK576_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOE60912.1"
                     /translation="MKKFINDPANLTKELLEGLALCYKGKVKLVSERIVMRAVPKDES
                     KVAIVTLGGSGHEPALSGFVGEGMLDASVVGDVFAAPGAPKLLEALRLLKRDAGIVLV
                     TLNHDGDRMSATKALRDAEKEGIKVREIVTHDDIGPGVDADPNEKRGLAGCIPLYKVI
                     GAAAEEGKSLEEIVDIGERFNRHIATLAVAMTGCTHPQNGEPITVLPDDEMEIGMGQH
                     GEAGGGRTKILTADETALRMIEPLVKASGVKSGDTALLIINGVGATTLMEMLIVYRKA
                     AQILADKGINLTSGACGEYLTVQEMAGFQMILCKLDPDHVTYLKAKARTPYWTA"
     gene            complement(13464..13775)
                     /locus_tag="PK576_04645"
     CDS             complement(13464..13775)
                     /locus_tag="PK576_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60913.1"
                     /translation="MTYHHTGIPVFEKMEGMSFVEPLKVWVTDAGASPYKVEWLYFEP
                     DSPMAAAIQDETHVAYVVDNIEEAVKGKSVLWPVCEVAPGLKIAFIYDQGLPIELMQV
                     G"
     gene            complement(13800..14399)
                     /locus_tag="PK576_04650"
     CDS             complement(13800..14399)
                     /locus_tag="PK576_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60914.1"
                     /translation="MNNADYYKDGKFDSEKGKQAYFDLMKKFNVPVYAELARPDGPFW
                     AVDFAKGDFAAFGMGGVIWCNELAEGYFGHDIYLLPCQSIAEHRHLPTTDKDGKPIRC
                     KIESWQVRHGYVYGFSEVGEPNLDKFPEAKAMLSKVQIPHLKSVHVEKWEADGKVHKL
                     AKPETWHFMMGGKDGAIVTEYATYHDGAGLRFSVPGVGF"
     gene            15026..16123
                     /locus_tag="PK576_04655"
     CDS             15026..16123
                     /locus_tag="PK576_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOE60915.1"
                     /translation="MRILSRYVLREFLVPLFYCLAGFLGIYVLFELFDSFNRLMEARP
                     PFLEVVAFFAGYVSPYLEWLFPAALLLGTLYTMWNFCRHSEITAMRANGIGFTAIVRP
                     LLVVAALMALAVAAINEFYAPHAAEQARAMRENRFKPLTNTEILNIPYYNQQARRVWR
                     INHMRSDQPNRIEGVRISFDRPDGSRQLDITCRSAEYLDGLWWLFYPQYQYFDELNNP
                     IENPKPQLADLVIRAMPELNESPRDFLLMNKAWEFYSVRDMLHYLKNHPHLEKHEVAS
                     KTFDVHARLAAPFSCLIITLFAIPAGMASGRQSVFKGVLLAVALFFGFYVVTNGCMVL
                     AKNGLMPVILGAWCANVIFLAAGLALFYRQR"
     gene            16138..16842
                     /gene="trmB"
                     /locus_tag="PK576_04660"
     CDS             16138..16842
                     /gene="trmB"
                     /locus_tag="PK576_04660"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOE60916.1"
                     /translation="MPMNAVPDTPTPAPAALRTHVIPISDPTRPFTPDILSNDPARPL
                     EVEIGCGNGRFLATRASRNPDTAYLGIERLLGRVRKLDKKAIRLNLGNLRILRLEACY
                     TFYYLLPRHSVRTVYVFFPDPWPKRRHHERRLFSPLFLDALWARLEYGGAIQIATDHR
                     DYFAEIRTRFAADPRFAEIPAMTRTDEEQTEFEQLFRNQGLPIYACAYRALPAEEPAL
                     PPLTLAPDMEPQAERE"
     gene            17097..>18488
                     /locus_tag="PK576_04665"
     CDS             17097..>18488
                     /locus_tag="PK576_04665"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOE60917.1"
                     /translation="MSHRFTELLTPRLPDQPACRAVSALPGASAALTAVLLARTAPAR
                     FALTVTPGAVELERVYGDLCALGQDRGVAPLLFPPLQEDDPEAAGSRMRVIRILQTEC
                     KPHAERDAAPTVIVTSLAALLHPVPDPQALAAASVHLRVGESFPFESLVAQRVESGYT
                     RVTEVEAPGQLAVRGGLLDIWPPAAPAPWRAEFFGDELEALRTFDPATQCSVAPGQEV
                     WLPPCSETGLATLHLETLLPPHASVLWLDHDLIRAGADAPPPSTTGHARPASRAPAFW
                     DTLAARINTREPWLQLFAGDPPPARTPTLDFAIAALPGLAEWSAENAHHPELLTGVRQ
                     RLLAELARAADAGQTVLLYADTPGTCELLSRELGPDTRVHVCRAPLSGGFTLPGLTVA
                     AQPDLYAAYKPVRSRRPPAAAVRGGRVENAADLEPGDLVVHVDHGIGRFLGSTEVEID
                     DSRSEVFTIEYAEG"
BASE COUNT         3278 a         6513 c         5313 g         3384 t
ORIGIN      
        1 cgggcgtccc tgtcgatcgt cgcgggtcga atgtcgcggg tcgacgatcc cgatagcgat
       61 cccgatcccg atagcgatag cgatcctgac cccgatttgg atgaaaccct gaaaccctga
      121 aaccctgaaa accagaaacc aaagggcagt tgaaagttca actgctcttt ttagggtacg
      181 tcaacggacg cccgccgact cgtcccccat gtcgtagagc gcgggcggga agcgcttgag
      241 gatgcggtag acctgaatca ggcccgaccg ctcaagcagt gtccgcgcct gccacacgtg
      301 cctgcaggcc agtgcggcac gggatgctgc ggccgcttcg acgacctgcg cggcctgcgc
      361 ctccgaaagc tggccgccca cacggtcggc ccgcgcctcg accgccagag cctccatcca
      421 cgccgtctgt ttctccgcac gcgccaactc ctccggcggc acctgttcgg tcacgcccgc
      481 gagcgccgca ccgcccccgg cctcgaaggc cgccagctca tagggttgca gggtcagccg
      541 gagcaccccg tcctcaaggc gctgcggctt cgcggaagag aaccgacgca cctcgcttgt
      601 cccggtcaag cggatcgcga cgggaacggg gaagcgttcc cggttcaccg catagaacag
      661 aaaccgctcc tgccccggca gttcccacac cgccaccggg tcacgcccgt cctcgcgagg
      721 ccggaacggc aacgccggca gcgcacggta ttcgcgcaag aagtccgtca gcagcggctg
      781 ccccagcgtg taggcgttgc cgccatccga aaggtactgg gcgtccgctt cggccaacgc
      841 cagcgcgtaa cgctccagat aatgcctccc ggcaggattc acgacgccgc tcatccaagt
      901 ccgtttggtg tcctccggaa agccgagcga cgcgggcggc acgacacgct ccgtggcctc
      961 gaagtatccc gcgccgaaca ggaaggccgt cttcccctcc gtgttgcgga gcgcgagcag
     1021 cgccgccgga tcaagcagtt tatcgcgtct tttcgaatcg gccagcggac cctcataggt
     1081 gtaggcccgg cggccatagg cgtgtccggc attgacaagc accacgccgt cgagagcatg
     1141 gagaagtttg ggggcgattc ccgcctcttc cccgaatccg aaattgttga ggtacacggt
     1201 gaggtccgga cgcgcgcgcc ggacgcggtc acggatcgct gcatgaagct ccatgatctt
     1261 gcggcaccgc caggcggtcc acgcgccacg ggcgttcgcc atcagccagt cgtagcgggc
     1321 acggaagcgc ttcggatcgt ccgcccgcgc acccgtcgaa agccccgttt ccttctcgaa
     1381 caggctgacc gttaggtcgt cgtatcccca gtcgagcgag tggaaattgt tcagcccggg
     1441 gttggcccac cccatcaggc gcaggctcac gccgcgaaag gcgggcgagt ccttgtagcg
     1501 gtccacgaac tcgccgatca tgccgaggta ccactcccgg tcaacgggat ggagcggatg
     1561 aaagcgcggg attgcgctcc agtctttgcc gcccttgccg tctttcgaga tgagcccgtt
     1621 gggcttcggc gtgatctgcc ccgtcggatc ggccagccga tccaactcgc ggcactcggg
     1681 atggaactcg ccgatgaacc cgaggccgta tttctcgcat ttcagcagca acacccgcac
     1741 cacgtcgaac gagtgaggct cgctgaagtc gatgttgtaa cgtgacgggt acatcgccat
     1801 ctggtagacc gccaccgtgg agacgagcgt gtcgccgccc accgacgcga tggaccgcgc
     1861 ccagcggtcg gccccgatca gaaaaccgcg cgggctccgg tcgggtgcgc cgtaaacggc
     1921 catgaagttg ctgccctcct cgtaccagtt gccgaacgaa cgcccgcccc gaggcggcac
     1981 actgggcaac ggcagatcac cctccacgcg gtagaggcgg atcttcgaca ccgccgcgcc
     2041 gcgccgcccg ttgtgcgccg tcacaaacaa gacccgcaga tcgcgggtgc gcggccagaa
     2101 caggagcgcg tgcgtctgca tccgccgcgt cagcgcgtag cactcgccac tgtcgacgcc
     2161 gcccgcgatg ggatatgcgt ccggagccgc ctcgcgaatc gcgatgcaga acgtgcggaa
     2221 cgcgtcgtcc ggatagtcgg cctccagcac gtacggcgct tgagcctccg gcacgcccag
     2281 ccgataggcg aaccagccgc actcctgctt gttgtgctgg gcccgtaaga atcccgcatc
     2341 gcccgattcg cggtagacgc cggcctcgct ccggaccacg ttcgcaccgc ccgtcccgga
     2401 gaactggggc gcggccaccg cgcagtcgat ctccgcaatc agcgctttcc gcagctcgcc
     2461 cccgcgcgcc gccggccggg tatcaaccac aacgaccggc cgaaccggca catcaaggtt
     2521 gtcgatccgc ctcccgtccg cggacacgcg gagttcgaac agcccctcgc gcggacaggc
     2581 cagcgtgacc ggctgttccg ccgggcgttc gcctaccgga acggcgacct ccgcctgcga
     2641 gattatctcc tgcgtcttgg ggtcgctcag caggatcgtc acccagccgg gctgtccgtc
     2701 cggcccgccc gcctgaagcc ggagcggcag cacctctccc gtgcgcacga tccggttttc
     2761 gcccggcatc catagacgga gccgtttggc gggtttcttc tccgccggcc gcaaggtgaa
     2821 ggattcgata cgctgaaacc ccgtccagtg atagcgctgg acgcgaaggg tgcgccgtcc
     2881 ggcctcaagc cagaaattac cgatcttgcc gctgcccgcc gagacagcga catcgccgtc
     2941 gaaccggtac tcgacacccc atgcctgccc cggaacaatg agttcatacc agtcggattg
     3001 cggcacctcg atgtcatact ctgcccagcc tttgatatag cagttcttcc cgggttcgga
     3061 cgtgcctgcc aaattgcctg agctgcggac gcgctccgcg tcttcgggac gggtaaactc
     3121 cgcgcggtca aatgaaccca tcggccgcac gatatccccg gcggcgcaaa aagcccatac
     3181 ggggccgccc atcagaagcg ctgtccaccc ccctcttctg aatcgtcccg cccgcatcac
     3241 gctttccctc atttcctgtc ctattccgac gaatcgataa aggtcacgcg gtccagcgtc
     3301 aggcgttcca caccccggat gttgaatgtg tttttgccgc cgggcagaag ctcgaagcgc
     3361 acgttactga gcagaatgtc gctgacccga tcccctttgc gtagcacaaa agagggatac
     3421 tcgggcgaac ggatcgtcat gtttgaaaac gtgatgttgc gaaggcggct gacctttgtg
     3481 tcaggggcga agctgacccg gatcgggtcc cggcagttca tgctgatatc gctgaaatgc
     3541 acgttctcaa cgccgaaagg ccgcacggca tccccttccg gcagaggcgg aacgcccggc
     3601 ccgcgcggcg gatcgttcgg gacgtgcgcc acggcaggca tcacgatgct gatccctgtg
     3661 atcgagtccc ggatcaccag attgctgaac cggcagtctt tgatctcata gtcgtgggtc
     3721 cacccgatgc ggatcgccga ggacctgctc tccagcacgc agttggccac aacgacccgc
     3781 tcgcaggcct tgggctcgtt caactggtgc tggtaggcgc gcaggatgat ggagtcgtcg
     3841 ctgctgcgga cgatacagtc gctgaccgtg acatcccggc aggagccgat gtggatcccg
     3901 tcgccgttcg ggaacttcag gttcatatcg atcttgagcc ctcggatgtt gacccgatca
     3961 caatccagaa gccacatgct ccagcccgcc gggttgtaga tcgagaagcc ctccagcgtc
     4021 acgtccgagc atcccaccag aaaaatgttc cgtcccgtga tcagctgatc gtgtttccgt
     4081 ttccaccagt gcccgctgcc ggacggctcg gtgaagagtt ccgcatggcc gttgattgtg
     4141 ccgcgcccgc agatcgtgag gttggtctgg gcgaccgcgt agaacatcgc acgcttgttc
     4201 cggcgcaggc tgtactcgac ccgccagtgg ggagacggaa cgaactccgg atagcgcaac
     4261 gggtcggggc cgccctccag cgaagcgccg gcctcgatct ccagccgggt gttcgagcgc
     4321 ggatagaggg tgtacgtcat gaacgctcca cccgccacac gcaccgttcc gccgcctgcg
     4381 gccgcgcagg cgtcaatcgc cttctggatc gccgccgtgt tgtccgtcac cccgtccggc
     4441 accgccccgt acgccgtgac ttgaaacacc ccgtcctgtc ccgcaccgcg tgccgtcagg
     4501 gccgccatca ccaaacaccc gatccatcgt ctcatccgga atcccccccc cctcgccttt
     4561 cacatcacat gtccgtttcc ttatcgccac ccgcgcccgc gctcctgcca cccatcctga
     4621 tcaccttccg ggcacccgct tcacccagcg ccggaaccag tccacgctgt gggacgcagg
     4681 cagggtggca ggatccaacc gcttaaccga agcccgccgc catgtggcga tctcgcagtc
     4741 gaacatcaca tgcagcggcg tgtggaaata atcgttgtcg cggcggaagt actctttgcc
     4801 gtccacatac cagaccatct ccttctccgt ccagagaaag ccgtagaggt ggtacccgtc
     4861 acacggtgcc cagtccaccg ccaccgcgcc gcccggattc gccaatttgg tcttcacatg
     4921 attgacaagc ccttcaacat acggcgtctc gaaccggtgc acatgcgcgc agatctcgta
     4981 gggcaaacag gagggatcct gatacttgcc gataaactcg taaatgtcga tctcctcgga
     5041 gtgactgccc ggacgaacct ttttggagag ccggtcgctc aaaggatcgt agagccagaa
     5101 ggcgttgcgc acctcggccg cgtttccgcg cgcccggcac tcgaaatagc cgtacgtcac
     5161 cttcttacgg ctcttgatga tcgcgcaggt gtacgggaga atcccgtcct cgcgatcctc
     5221 gtacgccatc tcctcggggt tcacgttacg cgcgtagagc gacagaaacc cgtcgcgcac
     5281 ctcgacattt ttgtggctgt agagaaactc ccgacgcccc cgccaacgcg gcgcataatc
     5341 ccaccacttc gccttgtcga tccccgtgcc gttgaattcg tcgctgacgg tcgcatccag
     5401 cacccagtcc gaacccaacg gacagaccgg ctcgtccgcc agaaccgccc cggttgccac
     5461 ccaaaacgcc acggatagaa tccgtcctgc tttcatcatc cacctctcct ttttttctgt
     5521 cccatcccct ttatcggctc aggaaatacc gggcccaaag cagatccggg cgttccttca
     5581 aagcctcccc gaaaagtttc ttggcctcct ccgaacgatt ctccgccaga gccagtagcc
     5641 ctttcagata aagcgccacg gccgtacggt gggtgacccg ttcggaagca gcgtcatctc
     5701 gagacactcg ttttccagcc ggagcgaggc gtacgtctga tccaccttct catcgtgcag
     5761 cgtgtcctgc aacggataag ggtagacgcg cccctgcgca ccctgataaa cgcggccggt
     5821 atagaagatc ggattcttgt cgtacggccc cggcggatag gtcggcaacg tcaccgtagt
     5881 ctcctccacc cgaacccgcg ccattccgtc cccgataccc agcagcacgc aaatcatcgc
     5941 cggaaccaac ggaaaacgtc tcatcccaat accctcttct tggttgtgaa gccagcatac
     6001 accctctgat caaacacaac aagtcacgtt tgcacagaaa accccgtgca ggaacggaaa
     6061 gcatcgtccc cgccctcgtt ctcgtgcaca tcggcccgcg acccgcgaca ttcggcctgc
     6121 gacgttgcgg cacgggcatc ctgcccgtgt ttcatgggcg ggaccccatg ttgccactga
     6181 tccactcctc gccccgcgtt gccgaccgga accagccgaa accgcttggc aagcagtggg
     6241 cgatggccgt atactaaagg gtcttgagag gttctgatgt tttttttcga caaaatacgc
     6301 cgtggtgaac cgacgatctc gttcgagttt ttccccccaa aaaccccgga cggctgggcc
     6361 cggctttacg ctacgatcgc cgaaaccgcc cggctgaatg tggatttcgt ttcggtgacc
     6421 tacggcgcgg gcggcagcac gcgcgaaagc acggtggagc tggtcgcctc gctgcagaac
     6481 aacctcgcga tccatgccat ggcccacctc acctgcctcg ggcactcgca ggctgagttg
     6541 gcccggattc tggatcgctt ggcagagaag cgtgtgcccg ccatcatggc gctgcgcggc
     6601 gacccgccgc agggcgagac cgctttccgg ccgcaccctt acggtttcgc ccatgcggat
     6661 gacctgatcg gttttatccg cgacggctgg ccggacttca agatcgggtg cgccgcctat
     6721 cccgaagggc accccgagtc cttcgccccc ggcgaggcgc gtaccccgga catcgacatc
     6781 ggcatcctcc gacggaaaca ggagaacggc gccgatttcg cggtcacgca acttttcttc
     6841 gataatgccg tctacttcca cttcgtcgac caagcgcgtg ccgccggggt gacgatcccc
     6901 ctgttgccgg ggatcatgcc gatgtcatcc gcaaagcaga tcgagcgttt cattgcccta
     6961 gccgggtgca gcatcccgga aacgctgaaa caggcggccc gggcaccgga tgtggccgaa
     7021 gcgggttttc ttttcgcgtt ggatcagtgc cgcgacctgt tgacgcgcgg cgcgccgggg
     7081 atccatctct attcactcaa tcaggcggaa ctctccagcc gcctgctcac cgcgctccgc
     7141 gatgagcgcc tccttcggta aagtggcttg acgctccgcc gacaacccgt catgctctcg
     7201 tcatggtcaa agacggacac atccgcctgc tccccattca catcgccaac aagatcgcgg
     7261 cgggtgaggt ggtcgagcgg cccgcttcgg tagtcaagga actgatcgaa aacgccatcg
     7321 atgccggggc cacgcgtctg gacatcacgg tcacggccgg cggacgcaag ctgatcgcgg
     7381 tctctgacaa tggctgcggc atgaaccgcg acgacgcgtt gctcagtatc gagcggcagg
     7441 ccaccagcaa aatcaccgat gtggatgaca tcgaacgcat cgacacgctc ggcttccgcg
     7501 gtgaggcgct gccgtcgatc gcctcggtgt cgcgtttcct gctcaagacc cgccgcgccg
     7561 aaaaggacgc gggcaccgaa ctcaccgtgg tcggaggcag tctccaggac gtgcgcgaag
     7621 ccggcctgcc gcccggaacc acgatcgaag tccgggacct gttcttcaat gtcccggccc
     7681 gccgcaagtt cctgcgcgca tatcaaaccg aacaggcgca catccggtcg gttttcaccc
     7741 tgcacgcgct ggcccatccc gaaatcggct tcagcctcgc cgcagacggt cgcgaactgg
     7801 cgcgcctgcc gtgcgacgcc acacacgagg agcggatccg cgatcttttc ggcgcggcgt
     7861 tctgtgccgg actgcgcccg gtggaccgga gcgtgcgcgg cgtgcgcgtc accggattcg
     7921 cgggactgcc gaacctcacc cgcacggacc gcagcgaaca gtacgtcttc atcaaccgcc
     7981 gccccgcgac cgcgccggtc atcgcgtacg cattgcgcga ggcctacccg cctctggaag
     8041 gcaaccgcaa accgatcgtg ctgttgttca tcgagcttcc gccggacgag gtggacgtga
     8101 acgtccatcc caccaaacgc gaagtccggt tccgcagacc cgccgacgtg cgcgaggcca
     8161 tcatcctcgc tgtccgtgcc gcgctcgggc ggaaacgcga gacgcgcgcc gccgctccag
     8221 cgggacatca ggacgaggcc gccgtgaccc tgcgcgcgct ggtgccggag gctgccgcgc
     8281 agacacgagg cgaacccttc ccctaccccg gaccggtggc gctgccgccc acggacgcac
     8341 cggcggcggc acccccttcc ccggccaagt ccctgccgac cgaaccgccg cccgccccga
     8401 ccggttcggg actgccgcta ttccacacgc ccgaagacgg ctcgccgtgg cgttggtgcc
     8461 gtgtgatggg acaagtcggc ggacggtatc tgttgctgga gaccgatggc ggatacgtga
     8521 cgctggaccc ctgtgccgcg cgcgagcggg tgctgtatga acggctgatg accgtacatc
     8581 gatccggcgg cgtggtctcg caagcgttgc tgttgcccga aaccgtgcgc ctgccgcccg
     8641 ccgatgccgc gcgactacgc aagttcctca ccgtcgtgca ggaaatgggt ttcggcatcg
     8701 cggatttcgg cagcgaccat tttatggttg agagcctgcc ggatgcgctg gccggtgtct
     8761 cctgccgtga actgctcggc aacatcgcgc aggatttgga aaatgtcgga acgcgccggg
     8821 gcgcggacaa gtggcgccaa gaactggtgg ccaaggccgc atgccgggcc gccgcaggtg
     8881 gcatggcgcc cctctccgac gatgcggccg agaagctggt ggcggatctg gccgaaacgc
     8941 agatgcccta cacctgtcct cgcgggcgcc ccaccatgat cttcacctcg tatcgtgagt
     9001 tggaacgcaa attcggacgg taatacggag gtgtttgtgc cttacgaacc aacaagcaca
     9061 atacaaaaca agtcgtttct cacacgaagg cacgaaaaag agtggtcccc gagccgggag
     9121 cgcgggcgcc cccgcccgct tcggaggaga gcgccacgtt ttgttgttgc gtcgttcgcg
     9181 ttggcctgcc gcgtcctgtg tgccgcagac gcggatgcgc aacccgcggc gcgtgtcaag
     9241 gcggtggtgc ggattctgcc ccaaacggtt gacgtccgta acgacgcgtt cctgatcggg
     9301 ctgaccgccg gatgcgacgg cggggccgcg cgagccgtgc gggccgaacg tttgccccat
     9361 gaaataccgg aagccgaact gcggagttca tgcccgactg gcggagcggt cagaatctcg
     9421 gtttcacacg tcccgggcga gaccgccccc ttttacacgg tggtgaacaa cagcgacacg
     9481 gagcagggcg tgacgctgct tgcccaagcc ctgcgccaag gtgtgcccca caccctgtcc
     9541 gtaatctgcc gcagcgagcg ggataagacc cgcctgaccg tgggattcca atctgcggct
     9601 gacccggccg cgccggagac gttccggcgg gcgcgtatcc cgtcgggcag caaatggaac
     9661 cgccacacgc tgaccttcac gcccgaccgc gacggcacct tccgtttgcg ttttctgctg
     9721 gagccgggcg ggatcgtcac cttcgcccgc ttttcgctga tgccggccga tgccgacgag
     9781 aacggctggg accctgtggc gctggaagcg ttacgcgtga ccgccgcgcc attcctgttc
     9841 tggccggtat gctctgggag cgggttctac aactggtacg acggcgtggg cccgcaggca
     9901 gcgcgcgaag cgaaaggcca agccttcggc acggtcgagg cggtccgtct cagccgcctc
     9961 tccggtgcag agccggtttt ccgaatttcc cttttcgctc cggaagacgc ctcggaagca
    10021 acggggatcc cccgtcccgc ggccgcagcg gaactcgccg cagattgggt ggcctactgc
    10081 aacgcgaccg gcagccatcc gctcgcgatg ctgcggaccc gacacggcgt gaccgaaccc
    10141 ctgggtgtac ggcggtggga actctgcccc gcagggggtt tgctgcggga tccttccgct
    10201 ttcgcccgcg aaagccgccg cgtggcagac gccatgcgcg cggaggatgc aaccctcacc
    10261 ctgcaaatca ggccggcttt gacaaatgcg tatcccgtgt cagacccgta taccggccgt
    10321 ttgctgaaac gccttgaatc agccgatgcg gcggaacgcg aatactaccg cgcatggtac
    10381 gaagcgctcg ggccgctcag cgcgcggctg gaccttcgtg ccgcggaatc cgatcgcgac
    10441 aacaccgctc cactcgcttt cacgctgccg gacgtgctgt gccgcaacgt atcccctccc
    10501 gcctgcctga cgggaccggg cctgctgatg accctgctca accgcttccc cctcgcggga
    10561 atcctcgcga cggagggcgc cccgccgacc gtgcgcattc tggcgggatg gggcgaggac
    10621 gactcggtcc tgcttttgtt cgtctgcaac accggcaccg aagcgcagac cctacatttc
    10681 gatctgcggg ccctgcgacg ccgcttcatg ttttacacgc tcgatcaggc cgccgcggaa
    10741 atcaccttgc cgcgtctgac tcccgtgctg ccgatccgcc gacatcagaa agccggtgcc
    10801 gcgctgacac agttcgtggt ctgcgaagcc gcgccctcct ccttcacgcg cattctggtc
    10861 accgagtgag aaacggaaac cgtcttgcgc gtcggctcac aaatcgttga acatctggaa
    10921 ggcgtccttc ggcttcagcc cctgctgaac caccgccgaa acggcttgaa tcatcgccgc
    10981 aggtttttcg gactggaaca cgttacgtcc catgtccacc cctgccgcac cgctatcgat
    11041 cgcccggtac gccagttcca gagcctcgag ttcgggcagc ttcttgccgc cagcgatgac
    11101 gatcggaacg ggacagcacg ccacgacctt ctcaaacccc tcggtgtagt aggttttgac
    11161 gatgttcgcg ccgttctccg cgcacagccg tgaggcgagc ccgaagtacc gcgcgtcgcg
    11221 tgccatatcc ttgccgaccg ccgtcacccc catcacgggg atcccgcaag cctgacccgc
    11281 atccacagcc tgatacaggt tggccacggt ctccgcctcg gtggccgcgt cacccactgc
    11341 aaccatcacc gcgattgccg aagcgttgag acgcaccgcg tcctgaacat cgatcaccac
    11401 gttgtggttg agttccgtca ggatcgaggt gcccgcgtcg gaacgcaggc agatcggctt
    11461 gcgacaggta ggcggcacaa gcgaacgcaa cacgccgcgg gcgcacatca gacagtcggc
    11521 atactcgatc agcggcacga tgctcaggtc gatacgctcg acgccggagg tcgggcccat
    11581 gatgaagccg tggtcgaacg ccagcatcac ggtccggccg gtagcggggt tgaagatccg
    11641 gctaaggcgg tccttcatgc cccagtcgag gtggctcgat cccttgagaa agaagccctc
    11701 ggttttctgc gccgtaccga cgccatactc tttaccatca cggatatcat ccaaatcagc
    11761 catttcatac tctcccgccg ggtctcccgg tcacttcgtt tccgccgcct tcgcgaacgc
    11821 cgcgaaaatg caggccatgg acgccgcgcc cgcgtcgcag ggaccgatcg agcgatcccc
    11881 gagattcttg gcgcgtccga atttcgcgcg caacgcgcag gtggactccg ctccccggtc
    11941 cgcagcttcg gcggccttcg ccagcatggc cggcacgcct ccgccttgcg cggcaaccaa
    12001 agccccggtg gcagggatca acacgtccat caacgtcttg tcgccaacgc gtgcgcgcgt
    12061 cgtaaagccg atctcctcca gccccccctg gaacaccgcg gccaacgccg cctcatccag
    12121 tgcggcggct ccttcgggcg cggcctcgcc cataccctga agccacgcgc cgtagagtgt
    12181 gctggtcgaa ccgcacgcct cgttttgcag cgcgtcgctc agtgccttga ggtagcttcg
    12241 gaaatccgcc ccgtcggctt gggacatggc gttgaacgcc gtgacgatcg cggtgccgtg
    12301 atcgccgtcc ccgccagcgg cggcatccaa ctcggagaaa cgcttctcct gcgcacggat
    12361 ggcgtcgcca gcggcggcga tcatcatttt gaacctcgct aacgtcagca tggagttcgt
    12421 ctctcctgat gtcgtcatgc cgtccagtag ggcgtacggg ccttggcctt cagatacgtc
    12481 acgtggtcgg gatcgagttt gcacaggatc atctggaagc cagccatctc ctgcacggtc
    12541 agatactcgc cgcacgcgcc ggacgtgagg ttgatcccct tatcggcaag aatctgcgcg
    12601 gccttgcggt agacgatcag catctccatc agggtggtcg cgccgacgcc gttgatgatc
    12661 agcagcgcgg tgtcaccgga cttgacgccg ctcgccttca caagcggctc aatcatccgc
    12721 aaggcggtct cgtccgccgt gaggatcttg gtgcggccac cgccggcctc gccgtgctgg
    12781 cccatgccga tctccatctc gtcatccggc agcaccgtga tgggctcgcc gttctgcgga
    12841 tgcgtgcagc cggtcatagc caccgccagc gtcgcgatat gacgattgaa gcgctcgccg
    12901 atgtccacga tctcttcaag gctcttgcct tcctcagccg cggcaccgat gaccttatag
    12961 agcgggatgc agccggccaa gccgcgtttc tcattcgggt cagcatccac accgggtccg
    13021 atgtcgtcgt gcgtcacgat ctcgcggacc ttaatgccct ccttctccgc gtcgcgcaac
    13081 gctttggtgg cgctcatgcg gtcgccgtca tgatttagcg tgaccagcac gatgcctgca
    13141 tcgcgcttga gcaggcgcag cgcttcgagc agcttgggcg cgcccggcgc ggcaaaaacg
    13201 tcgcccacca cactcgcgtc cagcatgccc tcgcccacaa acccgctcag ggccggctca
    13261 tgtccggagc cgcccagcgt gacgatcgca accttgctct cgtccttggg cacggcgcgc
    13321 atcacaattc tctcactcac gagcttgacc ttgcccttgt agcacaaggc caacccttcc
    13381 agcagttcct tggtaaggtt tgccggatca ttaatgaatt ttttcatcgc catcgcaatt
    13441 tctcccggtt acacgtttta caatcagccc acctgcatca gttcgatcgg cagaccctgg
    13501 tcgtagatga aagcgatttt cagccccggc gccacctcgc acaccggcca caggacgctt
    13561 ttgcccttaa ccgcctcttc gatgttgtcg accacgtacg cgacgtgggt ctcatcctga
    13621 atggccgcag ccatgggact gtccggctcg aaatagagcc actccacctt gtacggactc
    13681 gcgccagcat cggtgaccca caccttcagc ggctccacga acgacatccc ctccatcttt
    13741 tcgaaaacgg ggattccagt gtgatgatac gtcatgtttt gcctccttgt tgaactgatt
    13801 tagaaaccca cccccggcac cgagaaacgc aggcccgctc cgtcgtgata cgtcgcgtat
    13861 tcggtcacaa tcgccccgtc cttgccgccc atcataaagt gccacgtctc gggtttggcc
    13921 agtttgtgca ctttgccgtc cgcctcccat ttctcgacat gcacgctttt cagatgcggg
    13981 atctgaactt tggacagcat cgccttggct tccgggaact tgtccaaatt gggctcgcca
    14041 acctcgctga agccgtagac ataaccatgg cgcacctgcc agctctcgat cttgcagcgg
    14101 atcggcttgc cgtccttgtc cgtcgtgggc aggtggcgat gttcggcaat gctctgacac
    14161 ggcagcaggt agatatcatg tccgaaatac ccttccgcca gctcattgca ccaaatcacg
    14221 ccgcccatac cgaacgcggc gaaatctccc ttggcgaaat ccacggccca gaagggtccg
    14281 tctggccgcg ccagttcagc gtacaccggc acattgaact tcttcatcag atcgaaatag
    14341 gcctgcttgc ccttctcgct atcgaatttc ccgtccttgt aataatccgc attattcact
    14401 cgcttacatc ctttctgcgc cgcacctccg tggcactttg caactcctcc cgacgtctga
    14461 cagcccgctg aaaaacccaa caggcccatc cccatcccgg cgaccattac gccggtcaaa
    14521 acattcgaca aaacagcttt ctccattgta atcctccttt gcgggtcatc accggctcca
    14581 taccggattc cagaccccgc atacgcacga atcgtagtta aggcagtaag tttaccgttc
    14641 catccatcgc ctgtaaaggc gaaagaggaa tacccttcgg ttccgggttt ctggtttatc
    14701 gaaatcgcta tcgggatcgc ttccccgtcc ccattctcgt ccccgttttc gtgtacagcg
    14761 acttgcgacc cgcgacatcc gacctgcgac agggaggcct ccggtccgcc ctgtccaccc
    14821 tgtcagacga aaaacaagcc gttgcgggca gggacgtccg gctccagact cccgcgcgaa
    14881 tgcacccccc gccccgcgtt cccgactgaa acccccaacc cggaacctga aacccaagaa
    14941 ccgttgagcg ggcagatttc ggttccggtc cgtaataaaa cggattgccg aaccggcaaa
    15001 cgctaccgta tactgaacca cgtctatgcg aatcctgtcc cgatatgtcc tgcgcgagtt
    15061 cctggtgccg cttttctact gcctggccgg gttcctcggc atctacgtcc tgttcgaact
    15121 cttcgactcc ttcaatcggc tgatggaggc ccgcccgcct tttcttgagg tcgtcgcgtt
    15181 cttcgcgggc tacgtctcgc cctacctcga atggctcttc cccgccgcgc tgctcctcgg
    15241 cacactctac acgatgtgga acttctgccg ccacagcgag atcaccgcca tgcgcgccaa
    15301 cgggatcggg tttacggcca tcgtgcggcc cctgctcgtg gtcgcggccc tcatggcgct
    15361 cgccgtcgcc gcaatcaacg aattctacgc gccgcacgcc gcagaacagg cgcgcgccat
    15421 gcgggagaac cggttcaagc ccctgaccaa caccgagatc ctgaacattc cctactacaa
    15481 ccaacaggcc cgccgcgtct ggcgcatcaa ccatatgcgg tccgaccagc cgaaccggat
    15541 cgagggggtc cgcatctcgt tcgaccggcc ggacggcagt cggcaactcg acatcacctg
    15601 ccgctccgcc gagtatcttg acggactctg gtggctgttc tatccccagt accagtattt
    15661 tgacgagctg aacaacccga tcgaaaaccc caagccccaa ctggctgatc tggtgatccg
    15721 tgccatgccg gaactcaacg agtcgccccg cgacttcctg ttgatgaaca aggcctggga
    15781 gttctactcc gtgcgcgaca tgctgcacta cctcaaaaac cacccgcatc tcgaaaagca
    15841 tgaggtcgcc tcaaaaacct tcgatgtcca cgcccgcctc gcggcgccgt tctcgtgcct
    15901 catcatcacg ctgttcgcga tccccgcagg catggcctcg ggacgccaga gcgtcttcaa
    15961 aggcgtgctc ctcgcggtcg ccctcttctt cggtttctat gtcgtgacca acggttgcat
    16021 ggtgcttgcc aaaaacgggc tcatgcccgt gatcctgggt gcctggtgcg ccaacgtgat
    16081 attcctcgcc gcaggcctgg ccctcttcta ccgccagcgc tgagaaaccg aaaccgcatg
    16141 ccgatgaacg ccgtccccga cacgccgaca cccgccccag ccgcactgcg cacacatgtg
    16201 atcccgatat ccgaccccac ccggccgttt acgccggaca tcctgtccaa tgaccccgcg
    16261 cgtccgctgg aagtcgagat cggctgtggc aacggccgtt ttcttgcgac ccgtgcgtca
    16321 cgcaaccccg ataccgccta ccttgggatc gaacgcttgc tgggccgcgt ccggaaactc
    16381 gacaagaagg cgatccgcct taacctcggg aacctccgca tcctgcggct cgaggcctgc
    16441 tacaccttct actatctgtt accgcggcac agcgtccgca ccgtctatgt cttcttcccc
    16501 gatccctggc ccaaacgccg ccaccacgag cgtcgcctct tttccccgtt gttcctcgac
    16561 gcgctctggg cacgtctgga atatggcggc gccatccaga tcgccacgga ccatcgcgat
    16621 tatttcgctg agatccgcac gcgcttcgca gccgaccccc gtttcgccga aattccggcc
    16681 atgacgcgca ctgacgagga acaaaccgaa ttcgagcagc tcttccgcaa tcaaggcctg
    16741 ccgatctacg cgtgtgccta ccgtgcgctg cccgccgaag aacccgccct cccgcctctc
    16801 acactcgccc cggatatgga gccgcaagcc gaacgggagt gagggttggt ctctggttcc
    16861 agggcggggg gttcagtcgg aagcgcggga cggggagtgc gttttttagt cggacgagtc
    16921 gaatcgcagg tctccctgtc gcaggtcgaa tgtcgcgggt cgcaagtcga cgcacttgcc
    16981 ctttccgttc ctggtttctt gtgtttcatg gtacgaacgc cgcgtttccc tcatgcgttc
    17041 gttgcactct cgccgcaccc cgcgtttgtg ctatactcac ggctactgtg atctcgttgt
    17101 cacaccgttt taccgaattg ctgacaccgc gcctgcccga ccaaccggcg tgccgggccg
    17161 tgtccgccct gcccggcgca agcgccgcgc tcaccgccgt tctcctcgcg cgcaccgcgc
    17221 ccgcgcggtt cgccctgacc gtgaccccgg gagcggttga actcgagcgc gtgtatggcg
    17281 acctctgtgc cctcggccaa gacaggggtg tcgctcccct actcttcccc cccttgcagg
    17341 aggacgaccc cgaggccgcg ggcagccgga tgcgggtgat ccggattctc caaacagaat
    17401 gtaaaccgca cgcggagcgc gatgcggcgc cgaccgtgat cgtgacctcg ctggccgcgc
    17461 tcctgcaccc tgtgccggat ccgcaagccc tcgctgcggc ctctgttcac ctgcgcgtcg
    17521 gggagtcctt ccccttcgag agcctggtcg cgcaacgagt ggagagcgga tatacgcgcg
    17581 ttacggaagt ggaggcgccg ggccaactcg ccgtgcgcgg cggactgttg gacatctggc
    17641 cacccgccgc acccgcaccg tggcgcgcgg agttcttcgg cgatgaactg gaagccctcc
    17701 gcacgttcga ccccgcgacg cagtgttcgg tcgccccggg acaagaggtc tggctgccgc
    17761 cctgttccga aaccggtctg gcgacgctac acctcgaaac gctgcttccg ccccacgcct
    17821 ccgtgttgtg gctggatcac gatcttatcc gtgcgggcgc ggacgcaccg ccccccagca
    17881 ccaccggaca tgcccgcccc gcgagccgcg cacccgcttt ctgggatacg ctcgccgcgc
    17941 ggatcaacac gcgtgaaccg tggttgcaac tctttgcggg cgatccgccg cccgcgcgca
    18001 caccaacgct ggacttcgca atcgccgcgt tacccggact cgccgaatgg agcgcggaaa
    18061 acgcccatca cccggagttg ctcacgggtg tgcggcagcg gctgctcgcc gaactcgcgc
    18121 gtgccgcgga tgcgggacag accgtgctcc tctatgctga cacgcccggc acctgcgaat
    18181 tgctgtctcg ggaactcggc cccgacacac gcgtccacgt gtgccgcgct cccctctctg
    18241 gcggattcac ccttcccggc ctcaccgtcg ccgcgcaacc cgacctgtat gccgcgtaca
    18301 aacccgtccg cagccgtcgc ccgcccgcag ccgccgtacg cggcggccgt gtggaaaacg
    18361 cggcggatct cgaacccggc gatctggtgg tgcatgtcga tcacggcatc ggacgttttc
    18421 tcggctccac cgaggtggag atcgacgact cccgctccga ggtgttcacg atcgaatacg
    18481 cggagggc
//
LOCUS       DAOSLE010000044        18397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3263_length_18397_cov_8.418275, whole genome shotgun sequence.
ACCESSION   DAOSLE010000044 DAOSLE010000000
VERSION     DAOSLE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18397
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3263_length_18397_cov_8.418275"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK576_04670"
     CDS             complement(<1..593)
                     /locus_tag="PK576_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60918.1"
                     /translation="MRSNSVKQLKTVRAAQARSVADAALNAGGGVLRLAPNWVPRSFC
                     YPGRRLKLHPDDLYAFGLNRGGIDERWFGSTTEAANEGRLPDEGLSYVVFENKRVLLR
                     DVVAELGATIVGAKIWKKYRRWPVYSKFFDNLGPLPHHLHQDDKLAKLVGQEGKPESY
                     YFPPQHNNLGARFPYTFFGLVPGTTKADIVRCLERWN"
     gene            complement(634..3309)
                     /locus_tag="PK576_04675"
     CDS             complement(634..3309)
                     /locus_tag="PK576_04675"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60919.1"
                     /translation="MNKTFWVMVTGLAACLVACAELDKAILLDQDKLIEVLGKAETSD
                     NDRVTACQNLGWCGTRKAIEPLAGLLGDEKPFLRHAARYGLEMIPDPEVEVALCAAAE
                     RLTGPALVGVLQSLGNLRRCQSRRSWVSALLGGSRYAQAVEVLRARMTDPDKQVAGAA
                     IQALGKLATSEAVEALAPHLGACPAVARAYLEAAGRIKAKDPDAAAPLYAALRTAGAA
                     VPDTVRLAALRGEIITSGRRGLELWRASIASSDPDTVDVALRAVLDTPSCAKATVAFA
                     GALAEVPAVRIRLAAVLGRRGDKASVPALAALANGTGTECPADRLAAAQALAMLNDAS
                     ALPPLLALARHEQGTIANGAKDAIMGFAGKAADDAVLSMMTEADASVRLAGIDMAMRR
                     RMAVAVPTLARLTRDRDPAILNAAIKGVGELGSFEEIPVLLAAIAGNPQNENASRALA
                     TLCSRYARPRGGVVKVESAVYGNFENNLTKDVTENVQKLADAGSITIQASGRLCRWDG
                     FGEDPAPGKPKVLRMVYLFDGVKKSVQIRENDSAHLSGETLLPVAMDPLRAAYDAATG
                     AEKVVLFRVLAGLANEQGLAVARAAAAQNKDEALREAAIRVLAEWRTPEALEDAASFA
                     QQAPSDRLRILALRGFVRQLEQSFTIPFERQIRLLEQAGSWAVREEDKALLKAALAVT
                     RRMQAEQGFVPMFNGSDMKQWKGGDGWWQVKEGVLQAQSSEEKPCKKNSHLIWTGGEP
                     GDFELRAEFRLSPGANSGIQIRARNQEFGDSGYQADMNGGGNYVGYLYHPKQHLVGER
                     GAKVVIAADGKKTVERFADSKELQDKVFKGDDWNEYSIICKGPSITLFVNGMKTCEFE
                     DHRPDTPRKGHITLQMHAGPPMKIQYRNLRIKEFK"
     gene            complement(3349..4689)
                     /locus_tag="PK576_04680"
     CDS             complement(3349..4689)
                     /locus_tag="PK576_04680"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE60920.1"
                     /translation="MSKSEHGGFRRDGCGTLGRRAFLKGTLTAAGAVAMPTLIPASAL
                     GKNGAVAPSNRIVMAGIGLGGRGTADLRSWILPDPEAQFVAICDVQRSRRAAIKAMTD
                     KHYGNKDCKMYVEMMEVLERPDIDAILTATSDRLHATVSVRAMRAGKDVYTEKPASMT
                     IAEGQAVVQTARQYGRVYQAGMQRLSEANFIVCNELLRLGRLGEVKTVYAHLAPWGNV
                     NMSREWLAAEPLPDPDELDWDRWLGPCPWRPFNKHYVHGGWHAQHDFYTSVIGEWGSH
                     TFAQCHDALGLQDTSPVHYPYVSNPTADGMEMTFANGVRMIQKQEGWRGTCGVRYVGS
                     EGWCACADGYARPEVSNPALLADYNKILQDYITRTGHVVGPMRDFFQCIRRRTVTVAN
                     PVLMHRSMSTVHCANIAQWLKRDLTWDPVREEFVNDEQANRFRSRAAREGWQIC"
     gene            4987..6078
                     /locus_tag="PK576_04685"
     CDS             4987..6078
                     /locus_tag="PK576_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOE60921.1"
                     /translation="MYVVRCGDYGASLPVALNRLLDLTGWLRDGSLAGKRVLLKPNLL
                     TERSPEQAVTTHPELVRQMLRALKPAGARLAVGDSPASTANLENVWRATGMAAVCAEE
                     EVPLISFEQAGVLAFERNGFAFSVARPVLEADLIVNLPKVKSHSLTLLTAAVKNLYGA
                     IPGYAKTTLHRLHPRPATFGALLRTLWDVLPPVWNLADGIVGMEGQGPANGRPVQLGF
                     LAASRDPFALDRALCDLLHIDARRVPFLPPGDAVRVTGDPVSIATFATPSGGHLLTLA
                     PPWLVRWAGRCVWVRPAFSARACIRCGLCVKACPVHALSLAPEARTPTLDTARCISCS
                     CCHEVCPANAIRMSQSRLLRLLGVFKGLD"
     gene            complement(6097..6567)
                     /locus_tag="PK576_04690"
     CDS             complement(6097..6567)
                     /locus_tag="PK576_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE60922.1"
                     /translation="MVRFKEIIGHSTVCLNLDETDGPQVVARLIQAAVDEGRLPAERR
                     DEVVQTVLKRERGASTAMPDGIALPHGRTDCVRDILCVIGIHPRGIEFGAPDLEPTHI
                     FVLLLVPLTVGCNHISFLANLSRRLMEQSVRDNLVRALSREAVLRTLLEEGTES"
     gene            complement(6586..7551)
                     /locus_tag="PK576_04695"
     CDS             complement(6586..7551)
                     /locus_tag="PK576_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOE60923.1"
                     /translation="MKRIDFSNYLDALQQRRQPWQQGYYAMYSSVLGGVVRDPLLMQV
                     PLDDHLVHRGDGVFDTFKCVGRAAYNLEAHLQRLLRSASSIGLSWPDGIDGIRALTVE
                     TLAEAGREVCSGRVMISRGPGSFGVSPYESPAPALYIVVYAAGVPFMVRHPEGARVRR
                     SQVPVKPARFATVKHCNYLPNVLMKREAVDWGVDFVVGFDDDGLMAEGATENFGIVTR
                     AGELLFPRVETVLDGTTMLRVMQLAEPLAAEGALRAIGFRDITEADVRDAAEMLVVGT
                     TINVVSVREFEGVPIADGRPGKIGQTLNDLLTRDIETNAALRTVY"
     gene            complement(7776..10475)
                     /locus_tag="PK576_04700"
     CDS             complement(7776..10475)
                     /locus_tag="PK576_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60924.1"
                     /translation="MAQRTAGCRKRVVLFTLVAGGLILSSQAAWIGAGTAGGDPDATD
                     INDANNWQSGVIDGDFRTVVSNVDLRLTADYTATNGLDFSQTASVLRHITISGTNTLT
                     LNAEIPYYNFNKGSTHVFLPSNTASTVTLKRGLTLSVPSGTWHVRGYGTLFIDAKVTG
                     KGKLNTGTGSGRTYIVLRNGTNDFRGGTSGDTGRYNFTSIGNSGVPSAVGASGDISPN
                     NAPVAYIGSRSRSTDRLFQFNHNGARLVNDSACGSLNMLGIVRPTNWGGNWLSPMSFD
                     GISSGETLVTNNIANGSSTSYARLCKLGSGTLRLTGSNTFFCTDSSVHQVELLGGTLI
                     ADYADDVTGAGSNRLFLAGHTVSYDNGHLVIRGKTGAGNTTWQEFGTNTIGVSNAIRS
                     NVLTVDGNGGDGTTVQLGLIDMPSSYSFLLIERLGNASVRTAEAIPSDNGSVRVTNGL
                     VMCADGTRANILVKDPDGRTGFAVQDENLGLVRHTGTIALTPDNATAVSHVSLASDLT
                     RTANLTFSTLDIDASANPVTLNLNGFSFQTDNSVVGRGIVINGSNPVAILGGAHGAQS
                     THIYNYGTDKFTWGLTNSSGCTLVSAGPGLTEITQPVLSSLYIVGGVTRLSCGRNYTE
                     GAISIHGDGVLEIGADLNGASDGDFSRWSGTSSGQIGFIEGGGFSAYGADRTVIMNDS
                     ATRVVTWGESRFMVDGKPFVLSSPYANATLIFANAVNLGERAREFRVRNGSAAVDACL
                     TNRIYGIRVAALVKSGDGTLELKGPQDYPGECSVIAGGLKLGADDVFTGGVNALVLSG
                     ATLDAGTFRNTFNTLELLTDSVIEAGDGSATLAFADSSSETWTGTLTINGKLTAKTLR
                     FGTDGKGLSAAQLASITNRDRPVTLDEQGYLRQIPAGTVLLFF"
     gene            complement(10640..11983)
                     /locus_tag="PK576_04705"
     CDS             complement(10640..11983)
                     /locus_tag="PK576_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE60925.1"
                     /translation="MITNLFVLLGFAAVCSVFNGCALLRRRTLAPWAFALLYGVMGVV
                     VMLTPVVTHSGLTVDCRGGVLGTAALLIGPPVALICLLPPLAYRLALGSPHLLLGVGS
                     LVFPVLLGTLANLWQRRHDLSLTPRLVLSLSLLVGLGSNLLLALFAFCEGRGAVFDVG
                     VARALATLWITPVSMALLSALVLSEQRHITTVEQLAETERRMLGSQKMAAIGQLAYRV
                     AHTMLNALTVIQNRAELTKADPRSPKRVVANMDEVIRTVGTVSNLMGELVAFAIPDAL
                     RLRQMRLDTCLEGVERLLGKTIGEEVELVIRQGDVADGKVTIDPNRIEQLIVHLAINA
                     VEAVAGRGQVTISTYEISLSEEERTRLMAGLPACERHQGRFAVLEVRDTGCGMTAETA
                     GRIFEPFFTTKADRKNAGLGLPTVYAIVQQHHGFIDVQTQPGCGSTFLIYLPVTG"
     gene            complement(12044..12928)
                     /locus_tag="PK576_04710"
     CDS             complement(12044..12928)
                     /locus_tag="PK576_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOE60926.1"
                     /translation="MIMPKNADRLLMAFCPSANIVLRHAEVTDSARELEHSHLCGPTA
                     GLIQAEALAGVALLGAELTRPEEAVTLRLQVTGPIGGVLVEARSDGSLRGYTQVKVMN
                     DLDACEDLDAAVALGDSAEVRVIRSLPGVLLSSAGVEVRPASVPQGIETYYRQSQQRR
                     VRVQLTALAYGGFIDCARGVLIECLPDGDRAEFERIGRFVEDGTVQECLEAGASLQTI
                     CETVGVPDVQLAEPKPLRFHCPCSAERVSDMLGSMPTKELEEMICADQSVRVICHMCG
                     KGYSVAVEELKRIVASRS"
     gene            complement(12925..15039)
                     /locus_tag="PK576_04715"
     CDS             complement(12925..15039)
                     /locus_tag="PK576_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60927.1"
                     /translation="MKKTVHQFIARVRREARQKTEPVTVRSMGRDRVFYGYDWGVDTF
                     IFEKDVWKNLEEHSPVLIVRKSELIRRSGGHILTLTSGNHTVAAIPLLSGHFWNLGRV
                     PAARRSQVMREEVLCANVVNGNLEISQRDVPTRLLIKADEWLRNVGFALDEVIMAERT
                     DMTLDHYRRLGQEWRIKPLAWTRREMDAALRASRTRINTCLKYYHSAKGVHFLTYNDF
                     HALQTVLETDYPQFVACLRELVSIFEGDMRSFMRSPKFHGHHEIELFGLRRGEALKHL
                     VPELEKLMEGIALKRHTQEEVAEQFQVIDGMFRASLERPELADEASDDFVETLYMHLT
                     GEVYYGQGDSVSLAFDDRRTALPGATFRGGRPDFHPGADDRTRVLLANVEQMMSQDEM
                     IEYANIYEVRSDSDAANDRAVGAGVTREIVFKTNRQPLCTSLIEKRLALKMPGYGSYM
                     LARVQAFKALGVGFGEYRLLMRLDSSAGRALNYFIRNRCPGEPLDEIPPRMFQRSGEF
                     GGSEAGEDPDVVLALGALLGNAAAQNLVLKKYLPEDDSCRFGEGKEVFEFGYDIASMR
                     EMPMRVMLCSVRGSLGWPDLAFTEENLDRLFRFYFDAYARVLYRYGLKHRDAVAMDDL
                     TERFFDGFECKTREMHWNYSVRREQFDAFDPQLRHKYAFTRKWRFALWSLDRQLRRLK
                     PLRVLFRERMAALAAEAEEAGG"
     gene            complement(15094..17022)
                     /locus_tag="PK576_04720"
     CDS             complement(15094..17022)
                     /locus_tag="PK576_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE60928.1"
                     /translation="MTALPKRALVLAAGLGTRLRPLTQVWPKPLVPLWDVPLLEHVLR
                     LLESWGVEEVAVNLHWRPEAIRAYLETRRGGVRVTFSQEAELLGTGGALRPLRRFFGD
                     GPFWVVNADIAAAVDPQPFCEAFGGGHGLAAAWLEPKKGPRSVETDRRGRITCFRSPT
                     PGVPGTFTFCGLQLVSPRLYDFLPGRPFSTLVDAYTAAMAEGLFVDGVTVPGSYWDDA
                     GTVEALLRIHGEVKRLARRGKPGGALYRPEADRCDANATRFFCVGRDARVGRAVRGSD
                     SVVCGTARVGDASVLRRAVVAGGRVTGRLEDTVYVPLGGLDEASANRAVGALGWPVEE
                     TAVSCLGERGSNRSFWRLCRDARRAMLIRYSAERPENLRYAGHARLLADAGIPVPAVL
                     ADLPECGMLVLEDWGDDSLQRRMERRPGCAETWYAPVVKTLALWHRDVTRQVRECGAE
                     LEPAFDAELYAWERNLFETCLLVSRYGFDAIPAEAERELRGVADRLGRAPQVVVHRDF
                     QSSNILFRGKRFAFIDFQGMRRGAAAYDLASLLYDPYVILSVYVRNQMVAVYGKAYPE
                     HPEAVELFHDGAVQRLIQALGAFGRLASVGQRQFTRHIPRALESLLEAADLCGLDAVG
                     GLAEELIARERYRCRESG"
     gene            complement(17019..17504)
                     /locus_tag="PK576_04725"
     CDS             complement(17019..17504)
                     /locus_tag="PK576_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOE60929.1"
                     /translation="MGRGSGASAGALARNSVSGSGLDAVGGWRKNGVLVKGREAVDKL
                     ELRGLEVRCVIGDLPTERDREQVLTLDVTLACDLSAVCASDRVEDTVDYAVLAETIRD
                     ALRQARFRMIESAAARVAAVCLEAPRVSEVWVRIAKPGAVAGLREAAVELVRRRGDGA
                     S"
     gene            complement(17508..>18397)
                     /locus_tag="PK576_04730"
     CDS             complement(17508..>18397)
                     /locus_tag="PK576_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60930.1"
                     /translation="VPVYSCPYPGIRVNSSFSLQKGVPMLTVAGNALLGVLFTLLLGM
                     TDTASWGWSVFWGVIVFVAGQALAGYLIQRRVKSGMEAIQKILLDGQKRLQAKVNQWQ
                     NRPPGSLKQAQIEIEREQRVFVERALEASKGLERFKLWAPLMGRQINTLRLQFYWMLK
                     EFKQVDALLPNALFLDPMMAAVKLARMHMKDETEGMERFFRKQTRRLRYGQGALLYGL
                     YAWILVRQQDPDAAHKVLIEACEKMENETLKKNREHLANNRIDLFSNAGLGDEWYALH
                     LEQPKVKMQRQQRFSGRPF"
BASE COUNT         3387 a         6475 c         5304 g         3231 t
ORIGIN      
        1 ttgttccagc gctcgagaca gcggacgatg tccgccttgg tcgtgccggg caccagacca
       61 aagaaggtgt aagggaaacg cgcgcccaag ttgttgtgct gcggcgggaa gtagtaggat
      121 tccggcttgc cctcttgccc caccaacttg gcgagcttgt cgtcctgatg caggtggtgc
      181 ggcagcggcc cgaggttgtc gaagaacttg ctgtagaccg gccagcgccg gtacttcttc
      241 cagatcttcg cgccgacgat cgtcgcgccc agttcggcca ccacatcgcg caacagcacg
      301 cgcttgttct cgaagaccac atagctcagg ccctcatccg gcagacgccc ctcgttcgcc
      361 gcctcggtgg tcgagccgaa ccagcgctcg tcgataccgc cgcggttaag cccgaaggcg
      421 tagaggtcat ccgggtgcag cttcaacctg cgtccgggat aacagaacga gcgcggcacc
      481 cagttcggcg ccagccgcag cacgccgccg cccgcgttca acgcggcgtc cgccacagag
      541 cgcgcctgag cagcacgcac ggtcttcaac tgttttacag aattgcttct catgtgggac
      601 ccctttcttt tcggatcggt tacgacacct tgtttacttg aactccttga tgcgcaaatt
      661 gcggtactgg atcttcatgg gcggaccagc gtgcatctgc agcgtgatgt ggcccttgcg
      721 gggcgtgtcc ggccggtgat cctcgaactc gcaggtcttc atgccgttga cgaaaagcgt
      781 gatggacggc cccttgcaga tgatgctgta ctcgttccag tcatcccctt tgaagacctt
      841 gtcttgcagt tctttgctgt ccgcaaaacg ctcgaccgtt ttcttgccgt ctgcggcgat
      901 cacgactttg gcaccgcgct cgccgaccag atgctgcttg ggatggtaga ggtagccgac
      961 atagtttcct ccgccgttca tgtccgcctg atacccgctg tcgccgaact cctgattccg
     1021 cgcgcggatc tgaataccgg aattggcgcc gggactgagc cggaactcgg cccgcagctc
     1081 gaaatcgccc ggctcgccac cggtccaaat cagatggctg ttcttcttac aaggcttctc
     1141 ctccgaactc tgcgcctgca agaccccctc tttcacctgc caccagccgt cgccgccctt
     1201 ccactgcttc atgtccgaac cgttgaacat cgggacgaac ccttgttcgg cctgcatgcg
     1261 ccgcgtgaca gccagcgccg ccttgagcaa ggccttatcc tcttcgcgca cggcccaact
     1321 cccggcctgt tccaacagac ggatctgacg ttcgaacgga atcgtgaagc tttgttcgag
     1381 ctgccgcaca aacccgcgca atgcgagaat cctcaaccgg tcggagggtg cctgttgcgc
     1441 gaacgaggcg gcatcctcca gcgcctcggg tgtacgccac tccgcgagga cgcggatcgc
     1501 cgcttcacgc agcgcctcat ccttgttttg cgcggccgct gcacgggcca cggcaagacc
     1561 ctgttcgttc gccagaccgg ccaagacgcg gaacagaacg accttttcgg caccggtggc
     1621 cgcgtcgtag gcggcccgga gcggatccat ggccacgggc agcaaggtct cgccgctcag
     1681 atgggccgag tcattctccc ggatctgcac gctctttttc acaccgtcaa acaggtagac
     1741 catccggagc accttgggtt tgccgggcgc aggatcctca ccgaaaccgt cccaacggca
     1801 gaggcggccc gaggcctgaa tcgtgatgga tccggcatcc gcgagtttct gcacattctc
     1861 cgtgacatcc ttggtgaggt tgttctcaaa attcccgtag accgcactct caaccttgac
     1921 tacgccgccg cgcgggcggg catagcgcga gcagagcgtg gcgagcgcac ggctcgcatt
     1981 ctcgttctgc gggttaccgg caatggccgc caaaagcacc ggaatctcct cgaaggagcc
     2041 cagctcgccc actcccttga tggcggcgtt cagaatggcg gggtcccgat cccgtgtgag
     2101 ccgcgccagt gtgggcacgg ccacggccat gcgccgacgc atcgccatat cgattccggc
     2161 gagacgcacc gacgcatccg cctcggtcat catcgagagc accgcgtcat ccgcagcctt
     2221 ccccgcaaaa cccatgatcg catccttggc accgttggcg atcgttccct gttcgtgccg
     2281 ggccaacgcc agcaacggag gcagggcgga agcatcattt agcatagcga gcgcttgggc
     2341 cgcggcgaga cggtcggccg gacattcggt ccccgtaccg ttggccaagg ccgccagagc
     2401 gggcacggag gccttgtctc cgcggcgacc gagcaccgct gccaaccgga tccgcaccgc
     2461 aggcacctcc gccagcgccc cggcgaacgc cacggtggcc ttggcgcaag acggggtgtc
     2521 gagcaccgcg cgcaacgcga catccaccgt gtccggatcc gaactcgcga tcgacgcgcg
     2581 ccacagttcc agtccgcggc gaccggaggt gatgatctcg ccccgcaacg cggccagacg
     2641 caccgtgtcc ggaaccgctg cacccgccgt gcgcagcgcc gcgtagagag gcgcggccgc
     2701 atccggatcc ttagccttta tgcggccggc ggcctccaga taggcccgcg ccacggcggg
     2761 acaggcgccg agatgcggcg cgagcgcctc gaccgcttcc gacgtggcca gtttaccgag
     2821 cgcctgaatc gcggctcccg cgacctgttt gtcggggtcc gtcatgcgcg cgcggagcac
     2881 ctcgaccgcc tgcgcgtaac gcgacccgcc cagcaacgcg gacacccaac tgcgacgcga
     2941 ctggcatcgc ctgagattcc cgagcgactg cagcacgccg accaaggccg gtccggtgag
     3001 ccgttccgcc gccgcgcaca aggcgacctc gacctccgga tcggggatca tctccagccc
     3061 gtaccgcgcc gcgtgccgca ggaacggctt ctcgtcaccc aacaacccgg ccagaggctc
     3121 gatcgccttg cgcgtaccgc accagccgag attctgacag gcggtgaccc ggtcgttatc
     3181 cgaagtctcc gccttgccca acacctcgat cagcttgtcc tgatccagca gaatcgcctt
     3241 gtccaactcc gcgcacgcca caaggcacgc ggccagaccc gtcaccatca cccaaaacgt
     3301 cttgttcatc cttgttcccc gtatccccgt tgtgaaattc gtaaggtcct agcagatctg
     3361 ccagccctcg cgcgcggcgc gggagcggaa ccgattggcc tgttcgtcgt tgacgaactc
     3421 ctcccggacc ggatcccatg tgagatcccg tttcagccac tgcgcgatgt tcgcgcagtg
     3481 cacggtgctc atcgaccggt gcatcagcac ggggttggcc accgtgaccg tgcgccgccg
     3541 gatacattgg aagaaatcgc gcatcggacc gaccacatgc cctgtgcggg tgatgtagtc
     3601 ttgcaggatt ttgttgtaat cagccagcag cgcgggattg gacacctccg gccgcgcgta
     3661 accgtcggcg cacgcgcacc agccttcgct gccgacatac cgcacgccgc aggtgccgcg
     3721 ccatccctcc tgcttctgga tcatccggac cccgttcgcg aaggtcatct ccatcccgtc
     3781 ggccgtcgga tttgaaacat aggggtaatg gactggcgag gtgtcctgca agcccaaggc
     3841 gtcgtggcat tgcgcaaagg tgtgcgaacc ccactcgccg atgacgctgg tgtagaaatc
     3901 atgctgcgcg tgccacccgc catgcacgta atgcttattg aaggggcgcc acgggcaggg
     3961 gccgagccag cggtcccagt cgagttcgtc cggatccggc aacggttcgg ccgcgagcca
     4021 ctcccggctc atgttgacat ttccccaagg cgcgagatgc gcgtaaacgg tcttgacctc
     4081 acccagccgt cccagacgca gcaattcgtt acacacgatg aagttggcct cgctgagacg
     4141 ctgcatgcca gcctggtaga cacggccgta ctgccgcgcg gtctgaacca ccgcctgtcc
     4201 ttcggcgatc gtcatggagg ccggtttctc cgtgtagaca tccttgcccg cgcgcatggc
     4261 ccggaccgaa accgtggcat gcagccggtc gctggtggca gtgaggatcg catcgatgtc
     4321 gggccgttcc agcacctcca tcatctccac gtacatcttg cagtccttgt tgccgtaatg
     4381 tttgtccgtc atcgccttga tcgccgcacg gcgggaacgc tggacatcgc agatcgcgac
     4441 aaactgcgcc tccgggtccg gcaggatcca agacctgaga tccgctgtgc cgcgcccgcc
     4501 gagaccgatc cccgccatca cgatccggtt gcttggggcc accgccccgt tcttccccaa
     4561 ggccgacgcg ggaatcaacg tcggcatcgc caccgcgccc gccgcagtca gggtcccctt
     4621 caaaaacgcg cgccgcccca gtgtgccgca tccgtcacgc cggaaaccgc catgctctga
     4681 cttgctcatg cccttctctc cttttctgtt cttttcaatt aaagtcccct tttcagtata
     4741 gtcggcatcc tttccgcctt ttgatcgttg aatggtatcc gatctggtta ggtttgtcga
     4801 aaactcattt ttgtgaaatc gagaacggga aagacgggcg tccgctcgtc cgggcaggca
     4861 gacgcttgcc ccttccggcc tgtggcaccg ctatcgagat cgggaccacc tcctcgtccc
     4921 cgtctccgta tccggccgct ggcctccgac accgtgccct gctatactgc gcacatggat
     4981 ccggaggtgt atgttgtacg ctgcggcgac tacggggcat ccctgcccgt ggcgttgaac
     5041 cgtttgttgg atctcacagg gtggctgcgc gatggaagcc tcgcgggcaa gcgtgtgctg
     5101 ctgaagccga atctgctcac cgaacgctcc ccggaacagg cggtcaccac ccaccccgag
     5161 ctggttcgcc agatgctccg tgcgctcaag cccgccggcg cgcgcctcgc cgtgggcgac
     5221 agcccggcga gcaccgccaa cctcgagaat gtctggcgcg ccaccggcat ggcggcagtc
     5281 tgcgccgaag aggaggtgcc cctgatctcg ttcgagcagg ccggtgtcct ggcgttcgag
     5341 cgcaacggat tcgccttctc cgtggcgcgg cccgtgctgg aggccgatct gatcgtcaac
     5401 ctgccgaaag tgaaaagcca ctcgctcacc ctgctgaccg cggcggtaaa gaatctctac
     5461 ggcgccattc cgggatacgc gaagaccacg ctgcaccgac tccatccccg gcccgcaacg
     5521 ttcggcgcgt tactccggac attatgggac gtgctgccgc cggtctggaa tctggccgac
     5581 ggaatcgtcg gcatggaagg tcagggaccg gccaacggcc gccccgtaca gttggggttt
     5641 ttggccgcgt cgcgggatcc gttcgccttg gaccgcgcgc tgtgcgatct cctgcacatc
     5701 gatgcgcggc gggtgccttt cctgccgccg ggggacgcgg tgcgcgtcac gggcgatccc
     5761 gtgagcatcg cgaccttcgc cacgccgtcg ggcggtcatc tgcttacgct cgcgccgccg
     5821 tggctggtgc gctgggctgg ccgctgcgtg tgggtccggc cggctttttc ggcccgcgcc
     5881 tgtatccgct gcggcctgtg cgtgaaggcc tgtccggtgc atgcgttgtc cctcgcgccg
     5941 gaagcgcgca ccccgacgct ggacacggca cgctgcatca gttgcagttg ttgccacgag
     6001 gtgtgccccg caaacgccat ccgcatgtcc cagtcgcgcc tgctgcgtct gctcggtgtg
     6061 tttaaggggc tcgactgacc gcagaaacgg gacgcgtcag gattccgtcc cttcttcgag
     6121 aagggtccgg agcaccgctt cccgcgaaag cgcccgcacc agattatcac gcaccgactg
     6181 ctccatcagc ctgcggctga gatttgcgag gaaactgatg tggttgcatc ccaccgtgag
     6241 cggcacgagc aacagcacga aaatatgggt cggctccaga tcgggcgcgc cgaactcgat
     6301 tccccggggg tggatgccga tcacgcaaag gatgtcgcgg acacagtcgg tccgcccgtg
     6361 gggcagagcg atcccgtccg gcatggcggt ggacgcgccg cgttcgcgct tgagtaccgt
     6421 ctgcaccacc tcatcccgcc gttccgcagg cagacgtccc tcgtccacgg ccgcctgaat
     6481 caaccgggcc accacttgcg gaccgtccgt ctcgtccaga ttgaggcaga ccgtgctgtg
     6541 cccgataatt tccttaaacc tcaccatgaa ccgttcccct tgccctcaat acacggtgcg
     6601 caacgccgcg ttcgtctcga tatcgcgcgt cagcaggtcg ttcagtgtct gtccgatctt
     6661 gccgggccgg ccgtccgcga tcggcacccc ctcaaactcg cgcacggaca ccacattgat
     6721 cgtggtcccc accaccagca tctccgcggc gtcgcggaca tccgcctccg tgatatcgcg
     6781 gaacccgatg gcccgtaacg caccctcggc cgcgagcggt tcagcgagtt gcatcacccg
     6841 cagcatggtg gtcccgtcca aaaccgtttc cacacgcggg aagagcaatt cgcccgcccg
     6901 ggtgacgatg ccgaagttct cggtggcccc ctccgccatc aggccgtcat cgtcgaaccc
     6961 caccacaaaa tccacgcccc agtccaccgc ctcgcgtttc atcagcacgt tcggcagata
     7021 gttgcagtgc ttcaccgtgg cgaagcgcgc cggcttgacc ggcacctgac tgcgccgcac
     7081 gcgggcgcct tccggatgcc ggaccataaa cggcacccct gccgcataca ccacgatgta
     7141 aagcgccggt gcgggcgatt cgtagggact cacgccgaaa ctgcccggtc cgcgcgaaat
     7201 catcacccgg cccgaacaga cctcgcgccc cgcctcagcc aacgtttcca cggtaagcgc
     7261 gcggattccg tcgatcccgt caggccagga caggccgatc gacgatgccg aacgcaacag
     7321 ccgctgcaag tgcgcctcca gattgtaggc cgcgcgcccc acgcatttaa acgtgtcgaa
     7381 gaccccgtca ccgcgatgca caaggtggtc atccagcggc acctgcatca agagcgggtc
     7441 ccgcaccacc ccgcccagca cactggagta catggcgtag tatccctgct gccacggctg
     7501 acgccgttgc tgcagcgcat cgaggtaatt cgagaaatca atccgtttca tgtccatcct
     7561 tccgcgcggc gcggccgcgt ccggttcaat aagggatgga ggatagcata tccggtgccg
     7621 gccgccaaca gcgcggaccg ggaaagggaa gaaccggggc gcatcccctt ttcagtcggg
     7681 aacgcggccg tccccgccag tacggcggat cagtcaggtc ggactgacca gtcagatccg
     7741 aggcgcccct gccggtcgtc gcacgatggt caaatctaaa agaagagcag aaccgtgccg
     7801 gcgggtatct ggcgcagata accctgctcg tccagagtga cggggcggtc gcggttggtg
     7861 atggacgcga gctgggcggc cgagagtccc ttgccgtcgg tgccgaagcg cagagtcttg
     7921 gcggtcagct tgccgttgat cgtaagcgtg ccggtccagg tctcagagct ggagtcggca
     7981 aaggcgagcg tggctgagcc gtcgccggcc tcgatcacgc tgtcggtcag caattccagc
     8041 gtgttgaacg tgttgcggaa cgtgcctgcg tcgagagtcg caccgctcag cacgagcgcg
     8101 ttcaccccgc ccgtgaacac gtcatccgcg ccgagtttca acccaccggc aatgaccgag
     8161 cattcccccg gataatcttg cgggcctttc agctccagcg tgccgtcgcc gctcttgacc
     8221 agagcggcca cgcggatgcc gtaaatccgg ttggtcaggc aggcgtccac ggcggccgag
     8281 ccgttccgca cacggaactc acgggcgcgc tcgcccagat tgaccgcgtt cgcaaaaatc
     8341 aaggtggcgt tggcataggg cgaacttaag acgaacggtt tgccgtcaac catgaacctt
     8401 gattcacccc atgtgacgac ccttgtggcg ctgtcgttca tgatgacggt tcgatccgct
     8461 ccgtaggcgc tgaaaccgcc gccctctata aaaccgatct ggccactcga agttccggac
     8521 cagcgcgaaa aatcgccgtc ggatgcgccg ttcagatccg cgccgatctc cagaacgccg
     8581 tcgccatgga tggagatggc tccttcagtg tagtttcgcc cgcaggaaag gcgggtgaca
     8641 cctccgacga tgtacagtga actcaaaaca ggctgggtga tctccgtcag gccggggcct
     8701 gcggaaacga gcgtgcagcc gctgctgttg gtgaggcccc acgtgaattt gtccgtgccg
     8761 taattataga tatgtgtgct ctgcgcaccg tgcgcaccgc cgaggatcgc gaccgggttg
     8821 gacccgttga tcacgatgcc ccggccgacc accgagttgt cggtctggaa cgagaagccg
     8881 ttgaggttca gcgtgacggg attggcggag gcgtcaatat cgagcgtcga gaatgtgaga
     8941 ttcgccgtgc gcgtgagatc ggacgcgagc gagacgtggc tgaccgcggt cgcgttgtcg
     9001 ggggtcagcg cgatcgtgcc ggtgtgacgc acgagcccga ggttctcgtc ctggacggcg
     9061 aaccctgtcc gtccgtccgg atccttgacc agaatgttgg cgcgggtgcc gtccgcgcac
     9121 atgaccagac cgttggtgac gcggactgaa ccgttgtcgg aggggatcgc ctcggcggta
     9181 cgcaccgagg cattgccaag acgctcgatg agcagaaaac tgtaactgct cggcatgtcg
     9241 atgaggccaa gctgaacggt ggtgccgtcg ccgccgttgc cgtcaaccgt caggacgtta
     9301 ctccgtatcg cgttactgac accaatcgtg ttggtcccga actcctgcca tgtggtgttg
     9361 cccgcgccgg tcttgccccg gatgacgaga tggccattat cataactgac cgtgtgcccg
     9421 gccagaaaca atcggttgct gcccgctccc gtcacatcgt ccgcgtaatc ggcgatcagc
     9481 gtgccgccga gaagttcgac ctgatggacg ctgctgtcgg tgcagaagaa ggtgttactc
     9541 ccggtcagcc tcagcgtgcc tgatcccaac ttgcaaagtc tagcgtaact tgtcgagctg
     9601 ccgttcgcga tgttgttggt gacgagagtc tcgccggagg aaatgccgtc gaaactcatg
     9661 ggcgacaacc agttgccgcc ccagtttgtc ggacggacga taccgagcat attcaggctg
     9721 ccgcaggcgc tgtcgttgac gaggcgcgcc ccgttgtgat taaactggaa caaacggtcg
     9781 gtggagcggc tgcggctgcc gatgtaggct acgggcgcgt tgttagggct gatgtcgccc
     9841 gatgcgccta cggcggaagg gacgcccgaa ttaccgatgg acgtgaagtt gtagcggccc
     9901 gtatcgccgc tggttccgcc cctaaaatcg ttggtgccgt tacgcaggac aatataggtt
     9961 ctcccgctcc cggtgccggt attgagcttc cctttcccgg tcaccttcgc gtcgatgaac
    10021 agtgtgccat atcccctcac gtgccatgtg cctgacggca cgctaagcgt cagcccccgc
    10081 tttaaggtca ccgtgctggc cgtgttggag ggaaggaaca catgtgtgct tcccttgttg
    10141 aaattgtaat agggtatctc cgcgttcagc gtgagggtgt tggtgccgct gatcgtgatg
    10201 tgacggagaa ccgaggcggt ctgcgaaaag tccagcccgt tcgtcgccgt gtagtcggct
    10261 gtcagccgga gatcgacatt cgacacgacg gttctgaaat cgccgtcaat gacgccagac
    10321 tgccagttgt tggcatcgtt gatatcggtg gcgtccgggt cgccgcctgc ggtgcccgca
    10381 ccgatccagg ccgcctgcga cgacagaatc agtccgcccg caaccagcgt gaacagaaca
    10441 acccgtttac gacaacccgc tgtccgttgt gccatgacca acctccactt tttttcacaa
    10501 ccaccccatg ggatgccccg ggaacatccc ggttcccatg tttgacgctt attatgcgga
    10561 aaagaggcga aaagagtcaa ccctgtaaat ctgcaaaaaa gcggggattg ggcgtacgga
    10621 atcaggatcg gcggaccgct taccccgtca ccggcagata gatcaggaac gtgctgccgc
    10681 agccgggctg ggtctgcaca tcgatgaacc cgtggtgctg ttggacaatc gcgtagaccg
    10741 tcggcagccc caggcccgcg tttttgcgat ccgccttggt ggtgaagaaa ggctcgaaaa
    10801 tacggcctgc ggtctctgcc gtcatcccgc atccggtgtc ccgcacctcc agcaccgcga
    10861 aacgtccttg atgacgttcg cacgccggca accccgccat caaacgcgtc cgctcctcct
    10921 cggacaggga gatctcatac gtggagatcg tgacctgccc gcggcccgcg accgcctcca
    10981 ccgcattgat cgccagatgc acaatgagct gctcgatccg gttggggtcg atcgtcacct
    11041 tgccgtccgc gacatccccc tgccggatca ccagctcgac ctcttcaccg atcgtcttgc
    11101 cgagcagacg ctccaccccc tccagacacg tatccaaccg catctggcgc aaccgcagcg
    11161 cgtccggaat cgcgaacgcc accagttcgc ccatcagatt cgagaccgta cccaccgtcc
    11221 ggatgacctc gtccatgttg gcgaccaccc gtttcggact ccgcggatcg gccttcgtga
    11281 gttccgcacg gttttgaatc acggtgagcg cgttcagcat cgtgtgagcc acccgatagg
    11341 cgagctggcc gatcgccgcc attttctgcg aacccagcat ccgtcgctcg gtctcggcca
    11401 actgttcgac cgttgtgatg tgacgctgtt ccgagaggac caaagcgctc agcagggcca
    11461 ttgaaaccgg cgtgatccac agggttgcca atgcccttgc caccccgaca tcgaacacgg
    11521 cccctctccc ctcgcaaaag gcgaacaggg ccagcaggag attgctgccg aggcccacca
    11581 gcaggcttaa ggagaggacc agccgcggcg tcaaggacag atcgtggcgt cgttgccaca
    11641 ggttggcgag cgtccccaga agaaccggga acaccagcga ccccacgccc aggagcaggt
    11701 gcggcgatcc caacgccaac cggtacgcaa gcggcggcag caggcatatc aacgcgaccg
    11761 gaggaccgat cagcaacgcg gccgtcccca acacgccgcc gcggcaatcc accgtcagcc
    11821 ccgaatgggt gaccaccggc gtgagcatga cgaccacacc catgacgccg tacagcaacg
    11881 cgaacgccca cggggccaga gtcctgcgcc gaagcagggc gcatccgttg aacacgctgc
    11941 acactgcggc aaaccccagc agcacgaaca gattcgtgat caatccgcgt atgaactcgg
    12001 ccagattaat tatcccctcc tcgcgtaccc gctgccatcg ctctcaagag cgcgacgcca
    12061 caatccgttt caattcctca accgcaaccg agtatccctt gccgcacatg tggcagatca
    12121 cccgaaccga ctggtccgca caaatcatct cctccagttc ctttgtcggc atgctgccca
    12181 gcatatccga aacccgctcc gccgagcagg gacagtggaa acgcaacggt ttcggttccg
    12241 caagctggac gtccggcaca cccaccgtct cgcagatcgt ctgcagcgac gcgcccgcct
    12301 ccagacactc ctgaaccgtg ccatcctcca caaaccggcc gatccgttca aactcggcgc
    12361 ggtcaccgtc cggcaggcac tcgatcagca cgccgcgcgc gcagtcgatg aacccgccgt
    12421 acgccagagc cgtaagctgt acccgcaccc ggcgctgctg cgattggcgg taataggttt
    12481 cgatcccctg cggcaccgaa gccggccgca cctccacgcc cgcgctcgac aggagaaccc
    12541 cgggcagcga acggataacc cgcacctctg ccgaatcgcc cagcgcgact gccgcatcca
    12601 gatcctcgca ggcgtcaaga tcattcatga ccttcacctg cgtatacccg cgcaagctcc
    12661 cgtccgaacg cgcctcaacc agcaccccgc cgatcggtcc cgtgacctga aggcgcaggg
    12721 tcaccgcctc ctccggacgg gtcagctctg cccccagcaa cgccaccccg gccagagcct
    12781 ccgcctgaat cagcccggcc gtgggcccgc agagatgact gtgctccagc tcgcgcgcgg
    12841 aatccgtgac ctccgcatga cgcaaaacga tgttcgcgga agggcaaaaa gccatgagca
    12901 gccggtcggc atttttgggc atgatcatcc gcccgcctcc tccgcttccg cggccagcgc
    12961 cgccatccgc tcgcggaaca acacccgcag gggtttgaga cgccgcaact ggcggtccag
    13021 cgaccagagc gcgaaccgcc atttccgggt gaacgcatac ttatgccgca actggggatc
    13081 gaacgcatcg aactgttcgc gccggaccga atagttccag tgcatctcgc gtgtcttaca
    13141 ctcgaagccg tcaaaaaacc gttccgtcag atcgtccatc gcaacagcgt cgcgatgctt
    13201 gagcccgtag cggtagagta cgcgggcgta cgcgtcaaaa taaaaccgga agagccggtc
    13261 cagattctcc tccgtgaagg caagatcggg ccagcccaaa gaaccccgga ccgaacacag
    13321 catcacccgc atgggcatct cccgcatgct ggcgatgtcg tagccgaact cgaacacctc
    13381 cttgccctcg ccgaaacggc aactgtcgtc ctcgggcaga tacttcttca gcaccagatt
    13441 ctgcgcggcg gcgttgccca gcaacgcgcc cagcgccaac accacatccg gatcctcgcc
    13501 cgcctcactg ccgccgaatt cgcccgaacg ctggaacatg cgcggcggaa tctcatccag
    13561 cggctcgccc ggacaacggt tgcggatgaa gtaattcagg gcgcgcccgg cggagctgtc
    13621 cagccgcatc agcagccggt actcgccgaa ccccacgccc aacgccttga aggcctgcac
    13681 gcgcgccagc atgtaactgc cgtaccccgg catcttcagc gcgagccgct tctcgatcag
    13741 gctcgtgcaa agcggctggc ggttggtttt gaaaacgatc tcgcgcgtga cgcccgcgcc
    13801 gacagcgcgg tcgttggcgg catcgctgtc cgaacggacc tcgtaaatgt tggcatactc
    13861 gatcatctcg tcctgactca tcatctgctc gacgttcgca agcagcaccc tcgtgcggtc
    13921 atccgcgccg ggatggaagt ccggtcggcc gccgcggaac gtcgcgccgg gcagcgcggt
    13981 gcggcgatcg tcaaacgcca gcgagacgct gtccccctga ccgtaataca cctcgcccgt
    14041 gagatgcatg tacagcgtct ccacaaaatc gtcgctcgcc tcatccgcca gttcgggccg
    14101 ctccagcgaa gcccggaaca tcccgtcgat cacctgaaac tgttccgcca cctcctcctg
    14161 cgtgtgccgc ttcagcgcga taccctccat gagcttctcc agttccggca cgagatgctt
    14221 cagcgcctcc ccgcgccgca ggccgaagag ttcgatctca tgatgcccat ggaacttggg
    14281 cgaccgcatg aatgaccgca tgtcaccctc gaaaatcgaa accagctcgc gcagacaggc
    14341 gacaaactgc gggtaatcgg tttccagcac cgtctggagc gcgtggaaat cgttgtacgt
    14401 cagaaaatgc acgcccttgg cgctgtggta atacttcaga caggtgttga tgcgcgtgcg
    14461 gctcgcgcgc agggccgcat ccatctcccg ccgcgtccag gcgaggggct tgatccgcca
    14521 ctcctgcccc aaccggcggt aatgatccag cgtcatgtcc gtgcgctcgg ccatgatgac
    14581 ctcgtccagc gcaaacccca cgttgcgcag ccattcgtcg gccttgatca gcagccgggt
    14641 cggcacgtca cgctgcgaga tttcgaggtt cccgttcacc acgttggcgc acagcacctc
    14701 ttcccgcatc acctggctgc gccgggcagc gggcacgcgc ccgagattcc agaagtggcc
    14761 ggagagcagc gggatcgcgg cgaccgtgtg gttgccgctg gtcagcgtca gaatgtgccc
    14821 gcccgaacga cggatgagtt cgctcttccg gacgatcagg accgggctgt gctcctccag
    14881 attcttccac acgtcctttt cgaagatgaa cgtgtcaacc ccccagtcgt atccataaaa
    14941 aacgcgatcg cggcccatgg accgcaccgt gaccggctcc gtcttctgcc gggcttcgcg
    15001 cctcacgcgg gcgataaact ggtgaaccgt cttcttcatc cgctgccgta cacctttgtg
    15061 ccgccggtca acgcattcca ccggcagcac cccttacccc gactcccggc accggtagcg
    15121 ctcgcgggcg atcagctctt ccgccaaccc tcccaccgca tccagaccgc acaggtcggc
    15181 cgcctcaagc agggattcca gcgcccgcgg gatgtgccgg gtgaactgtc tctgccccac
    15241 ggaagccaga cggccgaagg cgccaagcgc ctgaatcaac cgctgcaccg ctccgtcgtg
    15301 aaacagctcg accgcctccg ggtgctccgg atatgccttg ccgtataccg caaccatctg
    15361 attgcgcacg taaacactta gtataacata aggatcatag agaagagaag ccagatcata
    15421 ggccgccgca ccccggcgca tcccctgaaa atcaatgaac gcgaaccgtt tgccgcggaa
    15481 gaggatgttg ctggactgga aatcgcggtg cacaaccacc tggggggcgc gtcccaaccg
    15541 gtccgccacc cctctcaact cgcgctccgc ctccgcagga atcgcgtcga atccgtagcg
    15601 gctgacgagc agacaggtct cgaacaggtt ccgttcccac gcatacagtt ccgcatcgaa
    15661 cgccggctcc agttccgcgc cgcactcccg cacctgccgc gtgacgtccc ggtgccacag
    15721 cgcgagcgtc ttcaccaccg gggcgtacca tgtctccgca cacccgggcc gccgctccat
    15781 gcggcgctgc aagctgtcgt ctccccagtc ctccagcacc agcatcccgc actccggcag
    15841 gtcggcgagc accgcgggca ccggaatccc ggcatcggcc agcagccgcg catgcccggc
    15901 gtaccgaagg ttttcgggac gttccgccga atagcggatc agcatggcgc gccgcgcgtc
    15961 ccggcacagc cgccaaaagc tccggttgga gccgcgctcc cccaagcagg agaccgccgt
    16021 ctcctccacc ggccaaccga gcgccccgac cgcccggttt gcggacgctt cgtccagccc
    16081 gccgagcggc acgtataccg tgtcttccag acgtcccgtc acccgcccgc cagcgaccac
    16141 cgcacgacgc agcacactgg cgtcaccgac ccgcgccgtg ccgcacacca cactgtccga
    16201 gcccctcacc gcccgtccga cccgcgcgtc ccggccgaca cagaagaacc gcgtggcgtt
    16261 tgcgtcgcaa cggtccgcct cgggccggta cagtgccccc ccgggtttgc cacgccgcgc
    16321 cagccgcttg acctcgccgt ggatccgcaa caaagcctcc accgttccgg catcgtccca
    16381 gtaacttccg ggcacggtca cgccatccac aaaaagcccc tcggccatcg ccgccgtata
    16441 cgcgtcgacg agcgtgctga agggccggcc gggcagaaaa tcgtagagcc gcggcgaaac
    16501 caactgtaac ccgcaaaaag tgaaggtgcc cggcacgccg ggcgtgggac tccgaaaaca
    16561 ggtgatccgc cctcggcggt cggtctccac cgagcgcgga ccctttttcg gttcgagcca
    16621 ggccgcggcc aaaccatgac ccccaccgaa cgcctcgcaa aacggttgcg gatccacggc
    16681 ggcggcgatg tccgcgttga ccacccagaa cggcccatcc ccgaaaaacc ggcgcaacgg
    16741 acgcaatgcc ccgcccgtgc ccagcagttc cgcctcctgc gagaacgtga cccgcacccc
    16801 gccgcgccgc gtctccagat atgctcggat cgcttcgggg cgccaatgca ggttcacggc
    16861 gacctcctcc accccccaag actccagcag ccgcaacaca tgctccaaca gcggcacgtc
    16921 ccacagcggc accagcggct taggccaaac ctgcgtcagc ggcctgagcc gcgtgcccaa
    16981 tcccgcggcc agcaccagcg cccgtttcgg cagcgcggtc atgacgcgcc gtccccccgg
    17041 cgccggacga gttcgaccgc cgcctcgcgc aaccccgcca ccgcgccggg cttggcaatc
    17101 cggacccaga cctccgacac gcgcggtgcc tcaagacaga ctgcggcgac cctcgcggcg
    17161 gcgctttcga tcatacggaa ccgggcctgc cgcaacgcgt cgcggatcgt ctcagccagc
    17221 accgcgtaat ccaccgtgtc ctccacccgg tcactcgcgc aaaccgcgct cagatcacag
    17281 gccagcgtca catccagcgt taacacctgc tcgcggtcac gctccgtcgg caaatcgccg
    17341 atcacgcacc gcacctccaa cccgcgcaac tcaagcttgt ccactgcttc ccgccctttg
    17401 accagcacgc cgttcttccg ccaacctccc accgcatcca gcccggaacc ggaaaccgag
    17461 ttgcgggcaa gggcgcccgc gctcgctccc gaccctctgc ccatccctca gaatggccga
    17521 ccgctgaagc gctgctgccg ctgcatcttg accttcggct gttccagatg cagcgcgtac
    17581 cactcgtcac ccaagcccgc gttggaaaaa aggtcgatcc ggttgttggc gagatgctca
    17641 cggttctttt tgagcgtctc gttctccatt ttttcgcagg cctcgatcaa gaccttgtgc
    17701 gcggcatccg gatcctgttg ccgcaccaga atccacgcgt acaacccata caaaagggcg
    17761 ccctggccgt aacgcagccg tcgcgtctgt ttccggaaga aacgctccat cccctcggtc
    17821 tcgtccttca tgtgcatgcg cgcgagcttg accgccgcca tcatgggatc cagaaaaagt
    17881 gcgttcggca acagcgcgtc cacctgcttg aactccttga gcatccaata aaactgcaac
    17941 cgcagggtgt tgatctgcct ccccatcagc ggtgcccaca gcttgaaccg ctctaaccct
    18001 ttcgaggcct ccagcgcgcg ttcgacaaag acccgctgtt cgcgctcgat ctcgatctgc
    18061 gcctgtttca ggctccccgg cggacggttc tgccattggt tgaccttggc ttgcagacgc
    18121 ttctggccgt ccagcaggat cttctggatc gcctccatcc cggatttgac gcgacgctgg
    18181 atgagatacc ccgccagcgc ctgccccgcg acgaacacga tcacgcccca aaaaacgctc
    18241 catccccatg acgccgtatc cgtcatcccc agtagcaacg taaacaaaac acccaacaac
    18301 gcgtttcctg ccacagtcag catgggcacc cccttctgca atgaaaaaga agagtttacc
    18361 ctgatgcccg gatacgggca actgtaaacc gggacac
//
LOCUS       DAOSLE010000045        18193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3299_length_18193_cov_8.639321, whole genome shotgun sequence.
ACCESSION   DAOSLE010000045 DAOSLE010000000
VERSION     DAOSLE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18193
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3299_length_18193_cov_8.639321"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..891)
                     /locus_tag="PK576_04735"
     CDS             complement(55..891)
                     /locus_tag="PK576_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE60931.1"
                     /translation="MTIIEPHIHMLSRTTDEYQAMYRAGIRACVEPSFWLGTNRRYAG
                     SFFDYFQLILEFETIRAARFGIDHYCAIAMNPKEADDRGLADEVIDGLVPYIEHPRCV
                     AMGEIGLNLNTPDEEYAMIRQLRMAEERKMPVILHTPHVDKARGTRRILEIIRAEGFT
                     VERIDVDHNTEETIRMTLDSGCYAGMTVYPYSKLTPQRVSDMVKQYGSDRVIVNGSAD
                     WGVSDPLSLIKVVEHMRGDGHSEETIRDLVFNTAMRFYSASPNWKPDFGIEPQDPRTY
                     QR"
     gene            complement(950..1897)
                     /locus_tag="PK576_04740"
     CDS             complement(950..1897)
                     /locus_tag="PK576_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOE60932.1"
                     /translation="MSKKKKRYAARLPQQVRGGIRAQSAGPQGGSTRVWWSRRWLAFL
                     ENYRLGARLGRGRSYAVAGQVSALRVEAGEVTAAVQGADKAPYRAEIRFAVLSEAAKA
                     RVTAALRQQPMLLARLLIGDLPERIEGLLQEEGCPLFPQREQDLSSRCTCPDYVNPCK
                     HLAAVYCLLGEAVTTDPLLLLALRGLDRVTLFGPETPGVRTPAEKAPHMESVPETGCG
                     AAEFYGTPQAAFEDFGPAPRAVLPAPLISRLGPLPFWRGQERFVDTLEPLYARAASRG
                     WTVWSGEPLDLRREEEKVILRGATLHLKQRRMRADPLGF"
     gene            complement(1894..4935)
                     /locus_tag="PK576_04745"
     CDS             complement(1894..4935)
                     /locus_tag="PK576_04745"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE60933.1"
                     /translation="MTILHSCWHASHLHIWGEVEACASGLPDGPRSAVAVSPFDAGAE
                     RLCHLLRPLMPGTGETPQPATLELFLPSRAWGSGFRPVPSQPFFNNAVFDGAGEEDAP
                     DFPHPWRVTAVVLPWRATLVLLGLVREPRLADELFIGESLLGFATLFRFAGALVARGR
                     FLPALHRTSEKVYEARWIPALDAADRKRLERLAPRLPPAAFAAGNGSPREALEELVDR
                     LVRASVVTTLSRAHAERGPWYSPHDAWFAALRGETRQVRWKTQAELEALAGALRQWRQ
                     PVESGEPGKVPRFTLEVPDGPEGAWFLRVLAEEGPEGEVPPPAWDAAFLLSLGQASLL
                     FPPLARAETRGGKFGCALSEEEAHAFLTVAAAVLEAAGYAVNLPPQWRPGHASALALE
                     ADLTPLTGEDPTTGTLSEARVAVTWSVTFNGTPLTKEEAVQLERAETPLVSLRGQWQT
                     LDLKRVREALRAQQRTDTVPVRQAVRLALGTEPGAGGLEIARARGRGWLDPLLDGLAG
                     GMSGMESLPVPTGFRGVLRPYQLRGFRWLAFLRAWGFGACLADDMGLGKTVQALAFLL
                     HEKDRGERRPVLLAGPMTVLGSWLNEARRFAPELRLLLHHGPGRAKGEDLARAAMDAD
                     VVVTSYALLHRDYAALRRIGWSGIVLDEAQNIKNPDTRQARAARALQADYRIALTGTP
                     MENHVGELWSVMDFLNPGLLGRRAVFRETFFRPIQAGTDPGARARLRRVTAPFILRRL
                     KTDRRVIADLPAKIEAKVFCPLTREQAELYEEVLESFRRDAEASEGIERRGLILAVLT
                     RLKQVCNHPAHFLGQSENLARRSGKLERLVERLEEVFARGESALVFTQYAVMGKLLAH
                     HLCRTFARETPFLHGGLTRPERDRMIRAFQNGTRPQAFVLSLKAGGTGLNLTRANHVF
                     HFDRWWNPAVENQATDRVFRIGQAREVMVHTLLCNGTLEERIDALIADKTGLANEVVV
                     SGEEFLTGLPDDALLEVLKLDAEVVDEEDAAPEQGGWGGWA"
     gene            complement(5027..8149)
                     /gene="priA"
                     /locus_tag="PK576_04750"
     CDS             complement(5027..8149)
                     /gene="priA"
                     /locus_tag="PK576_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE60934.1"
                     /translation="MATEISADRLFDYAVPDALAPRLRPGQRVRVPFGKRTLDAYVVE
                     IAEDTALEPGPPELFSLSGGSDKTDGIRTRRLRPILAIEDDVPFLSPTLIALARWMAD
                     YTCSPFEAALRCMVPAAVRNRDIKVKERFYVTPCPGEFALTSRQNELLENLRRVDGGW
                     LHSLTREFACAPQTLRTLAAKGAAVIERRAVRRDPLANRKVLPTRPLALMPEQASALK
                     LIIAAVDAGVRPESGARASPPAHAGASQTAGRPADVSPAGGDARAPDLTPDLTKDGTN
                     ATSLKFPGMIHSEPGTEAHWHSRGYVPHFDYPGLVQFITFRLVDSLPSSVIDAWKSEL
                     GIVNIVPTPPSANEELIRRIARYEDAGHGACYLKQPEIAREVSEVLRHGDGTKYRLLA
                     WSIMPNHVHALIEVEQADSVAQIVHDWKSVSAHRINRCLGRKGALWQPEYFDRYVRDP
                     NHFHSVVAYIQANRGIESGERTDLCAMQGEGAESGARASPPAHAGASQTAGRPADVSP
                     AGGDARAPDSAHAPKPVLLFGVTGSGKTEVYLQAIAHALQRGLGAIVLVPEIALTPQT
                     VQRFASRFGKRIAVLHSALGDGERFDEWHRIRNGEATVVVGPRSAIFAPVRNLGLIVV
                     DEEHEPSYKQDEVPRYNARDVAVMRGWMEHCAVVLGSATPAMESWLNVEKGKYVAAHL
                     TQRVAGRPMPRVQVVDMRLETARSGHAQLFSEPLLEAIRLRLERGEQTILFLNRRGYA
                     NALLCPRCGYAAACDACSVTFTYHRADACLRCHICGGWKHVPDVCPLCGDPAFRYAGF
                     GTQRAETALAACFPQAKILRMDADVTSRRRSHDEILAQFKSGRADILIGTQMIAKGLD
                     FPNVTLVGVLAADMSLHMPDFRAAERTYQLLAQVSGRAGRAELPGEVLIQTYSPGHPA
                     ICAAASDAGFEPFAARELAEREAGGYPPYARLICLTFRGASEEKVRFSAEAFARALSK
                     TTGNAAAVRISGVCAAPLAKAKGLYRYQLLLRAPTTRQITRPLREVMRRPLLASGVTL
                     AVDVDAINLM"
     gene            complement(8243..9004)
                     /locus_tag="PK576_04755"
     CDS             complement(8243..9004)
                     /locus_tag="PK576_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE60935.1"
                     /translation="MTRHQKDGFTLVELLVVIGILGILSAALFPAIGNAVMAANMAAV
                     GTRGKDIFVAITSANMEREPLGLGNVWPDEMSKATGTLSQDDQDRQTASTDSTKYFAY
                     LYDEENAGTTDWSPVAEGFDYSKLAGAGIPSCGKRNLSEEYNMWTIACNLRDEMEDVI
                     PILVTRNIDASSLAAKYNDNKANTKLNFSSSDPKPFSNKGFVMVRKGGAILKGRGKYR
                     EMRVIYNNQQFDTTLQQDAQQLKYLTPKTSVTPGG"
     gene            complement(9168..9683)
                     /locus_tag="PK576_04760"
     CDS             complement(9168..9683)
                     /locus_tag="PK576_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60936.1"
                     /translation="MKRSIRVGLIALVWVAGWVQAQPPPRAGRPRPPRTINRSNWVGK
                     HVLSPEFMEQVGLRGAQAEQLKAELEEIQAQAQRLEETINREALRQGELARNVLSEPG
                     ASVDEIMAIIERIGRHRTEQAKLATRQLVVIRDRLTAEQRKKARELIAAEARRRMAQR
                     AAERAARERAP"
     gene            complement(9870..12371)
                     /gene="mutS"
                     /locus_tag="PK576_04765"
     CDS             complement(9870..12371)
                     /gene="mutS"
                     /locus_tag="PK576_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOE60937.1"
                     /translation="MAGDKLTPMMRQYRRIKAELPQDVLLLFRLGDFYEMFFEDAREA
                     APILGVALTRRTGIPMCGVPHHALDAYLAKLIRAGRKAAICDQMEDPAQAKGIVRREV
                     TRVVTPGTVTEDEILDAARNNYLAAVYAGKGLGLALLDLSTGALTVEEAPDAAVLAEQ
                     LNRAAPSECLVGRDQRNDPRLAALLAPDAPGMVTEIDDWLLEYDCAYDRLLRHFGVHS
                     LEGYGCDGRAAQVCPVGALLHYVHEELRHPVGHIRTLRVRRAHEFLTLDENTCLNLDL
                     IPPRGQSPQATLLGVLDHTRTPMGARMLRAWLTRPLRQAEAIRCRLDAVDRLTKQRTL
                     LNELRESLGTVRDLERLIARLGAGHGNARDLNALALSLHPVPGLRERVAAAGDPLLAE
                     LAERLHALPELVGLITAAIAETPAAGLKDGGIIRAGYHAELDELRALATEGHAWLAAY
                     QASEQERTGIKTLKVRHNKVFGFYIEVSKGQAGAVPPEYQRRQTLVNAERFITPELKT
                     YEQKIFGAQDRATALEFELFCGIREQAVEQTAAIQETADALAHLDVLAVFADRALALG
                     YVRPEITEDDTLEIRDGRHPVVEQLPDAGPFVPNDTRLDGHRNQIMLITGPNMAGKST
                     YIRQVALIAILAHTGSFVPAREARIGLLDRVFTRVGAGDDLARGRSTFMVEMQEAANI
                     LNNATKRSLIVLDEIGRGTSTFDGISIAWAVAEYLHNTPRVKAKTLFATHYHELTDLA
                     LTLSGVKNYTVQVREHGDTVVFLRRIAPGTADKSYGIHVARLAGMPDEVIDRSREILA
                     NLEEGELESGLPKLARRSRRRNDDDIPGQLTLFDL"
     gene            12545..13897
                     /locus_tag="PK576_04770"
     CDS             12545..13897
                     /locus_tag="PK576_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HOE60938.1"
                     /translation="MIASPVYLASVVALAVAVLLILIIRLKQQPFVSLLLVSMGTALA
                     AGMPPEKVMACIEKGMGGTLGFIAVVVGLGAMFGQLLEVSGGAERLTLTLLRVFGKER
                     ATWAMALAGFLVAIPVFFDVGFIILVPLVYTLSRETKKSLLYYGIPLLAGLAVTHAFV
                     PPTPGPIAVAQLVGADLGRVIFYGVLIGLPCAIVAGPVFGTWIDRRIHAAVPAYMETE
                     AAKKQWDEKALPGFGRVFGILLLPLFLILLNTVTQAVLAEGEYATVRHVFAFIGHPFF
                     ALLAATLLAFTFLGTCRGLTREKVNEVATSALAPAGLIILVTGAGGVFKQVLVDSGAG
                     KALAGTLAHTGLHPVWTAFLIALLVRAVAGSATVAMLTAGGIMAPLLAGQRVEPALIV
                     IAIASGATALSHVNDSGFWLVNRYFGLSVKETLRSWTVMETLIGLIGATLAWLFSYVA
                     "
     gene            complement(13914..14930)
                     /locus_tag="PK576_04775"
     CDS             complement(13914..14930)
                     /locus_tag="PK576_04775"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOE60939.1"
                     /translation="MKQYSVGLVGIGAVGTEMIKVLRQRKFPAREIRIMATRERDEEV
                     AGETFHVIEARPEAFDGLDIVLFAGTEGSKGASKQFGWEAVRRGCVVIDNGDDFRMDA
                     RVPLVIPEINGEALTAHQGFIANPNCSTIITLMGIAPLHRRVPLRHMTAVTYQSVSGT
                     GRAAVEELEQQVRAYAAGNEPQVCAYPHPIAFNVLPQIGGAKPEMPGFTSEEAKLTFE
                     ARKILGCPDLRIAATCARVPVFYAHSVAVHAEFSAPLAPEEARAILANAAGIRVIDDL
                     AAAQYPMPLNTGGGDTVNVGRIRADPSVENGLALWCCGDNIRKGAAQNAVQIAEELIK
                     RGLL"
     gene            complement(14952..16229)
                     /locus_tag="PK576_04780"
     CDS             complement(14952..16229)
                     /locus_tag="PK576_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE60940.1"
                     /translation="MKRQWIGWVMAGILAAGIAADAPAGEEGFVTLFDGKTLNGWIQR
                     GGKSPYRVEDGAIVGCFGDAGRNSFLCTEKEYGDFILRLEFKFEGGNSGVQFRSAARK
                     HKDGSDSVYGYQAEIAGRSPVTARIYDEGRRGFKFKRTWLDNTPAEVTAKSVAAYKQG
                     DWNRMEIQCVGPSIRTWLNDVPVVNYFDDAALAGFIGLQVHAIKNPDGKPCARWRNIR
                     IRDLGTSRWTPFFVKGEDGRYALRGARFVLPEDWSFNHEEGILIGQHKEGEKRDGLVV
                     SDRNYSDFIVRVTYQLFGGNSALYFRAEEVNTPWLLKGFQNEIAGNAKDSALWHTAGD
                     KTKGRGWVAQNDEFIAQVRNPKGWNTTCTVAYGDRILSILNGQRTVDILDPLCEKSGK
                     VALQLHGGANGKMWFKDFEILEITPEMRALIDR"
     gene            complement(16337..18004)
                     /locus_tag="PK576_04785"
     CDS             complement(16337..18004)
                     /locus_tag="PK576_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE60941.1"
                     /translation="MTLRKIFEDTASRRPAAIALRYHRDQAWQSRTYESLMLGVRQMA
                     EAFGRLGLKPITERVAMIMENGPEWIETYLATAGAGVAVVPIDPKLRAAEVSYILNDS
                     EAVAVLTDTRHLDMLEVILTDLPQVRFIVVTDGAGERLAPIAGRPCYDYETLRAGVAN
                     EPLLWYADHRPDPQDVASIIYTSGTTGTPKGAMLTHLNFCSDAFGCFDAIGDVITAED
                     DFLVVLPLFHAFSFTANFVVPLVRGCGMSFIASLRSIGEDIRTLRPSILIAVPLLVEK
                     MFNKIDDKLRRNRIARLLFRLGLGCWVGKKVLKGLGGRLRFLIVGGAPCPLYVLNGFR
                     RLGVPIVEGYGLTECSPVVSITNSKNSRVGTIGKKLPNIEVRIADPDPQGVGELQIRG
                     PIVMKGYFKNLEATREAFDGEWLRTGDLAVQDAEGYLTICGRKKALIVNREGKNIYPE
                     EVENVLSRDPLIADVVVIGYTVGGIPGERVGTIVVPDLEAVAAAHEGVEPPWETVERL
                     IRERVRTQCECLTEYKHPRKIKVRREPLERTSIQKVRRCVYQGQLNE"
BASE COUNT         3158 a         6032 c         5623 g         3380 t
ORIGIN      
        1 gaaaccctga aaccctgaaa ccaaagagcg ttgctttcaa cgctcttttt aggtttaccg
       61 ctgataagta cggggatcct gcggttcaat gccgaaatcg ggcttccagt tcggcgaggc
      121 gctgtaaaag cgcatggcgg tgttgaaaac cagatcccgg atggtctcct cgctatggcc
      181 gtccccgcgc atgtgttcca ccaccttgat gagcgaaagc gggtcggaga ccccccagtc
      241 ggccgagccg ttgacgatca cgcggtcgga gccgtactgt ttcaccatat cggagacgcg
      301 ttgcggcgtg agtttagagt agggatagac ggtcatgccg gcgtaacatc cggaatccag
      361 cgtcatccgg atggtctcct cggtgttgtg gtccacatca atgcgttcga ccgtaaagcc
      421 ctcggcccgg atgatctcca gaatgcgccg cgtgccgcgc gctttgtcca catgcggcgt
      481 gtgcaggatg accggcatct tacgctcctc ggccatacgg agctgacgga tcatcgcata
      541 ctcctcgtcg ggggtgttga ggttgaggcc gatctcgccc atggccacgc agcgcggatg
      601 ttcgatatac ggcaccagcc cgtcgatcac ctcgtcggcc agcccgcggt cgtccgcctc
      661 cttggggttc atcgcaatcg cgcagtaatg gtcgatgccg aaacgcgccg cccgtatggt
      721 ctcgaactcc agaatcagct ggaaatagtc gaagaacgat ccggcgtacc tccggttggt
      781 cccgagccag aaagagggct cgacacaggc gcggatcccc gcgcggtaca tggcctgata
      841 ctcatccgtc gtgcgcgaca acatgtggat atgcggttca atgatcgtca tcttgttccc
      901 cttgttcatc ctatgaaacg ttccgtcctc cgttcgggtt caccggcggt cagaatccca
      961 gtggatcggc ccgcattctg cgctgtttga ggtgcagggt cgcgccgcgg aggatgacct
     1021 tctcttcctc gcgccgcaga tcgagcggct cgccggacca gacggtccac ccgcgtgagg
     1081 ccgcgcgtgc gtacagaggc tccagcgtgt cgacgaagcg ctcctgcccg cgccagaagg
     1141 gcagcggccc caaccggctg atcaggggtg cgggcaacac ggcgcgcggt gcgggcccga
     1201 aatcctcgaa cgccgcctgt ggcgtgccgt aaaactccgc cgcgccgcat cccgtctccg
     1261 gcacactctc catatgcggc gccttctccg ccggagtccg gacgccgggc gtctccggtc
     1321 cgaacagggt cacccggtcg aggccgcgca atgccaacag cagtagcgga tccgtagtga
     1381 cggcctcgcc cagcagacag tagaccgcag cgagatgttt gcacgggttg acgtaatccg
     1441 ggcaggtgca gcggctcgac agatcctgtt cgcgttgcgg gaagagcggg cacccctctt
     1501 cctgcaacag gccctcgatc cgctcgggca ggtcgccgat cagcaaccgc gccagaagca
     1561 tcggctgttg ccgcaacgcc gccgtgaccc gcgccttggc tgcttccgac aggacagcga
     1621 aacggatctc cgcgcgatag ggcgccttgt ccgccccctg tactgcggcg gtcacctcgc
     1681 ccgcctcgac acgcagcgcg gacacctgtc ccgcaaccgc ataactgcgg ccgcgaccga
     1741 gccgtgcgcc aagccgatag ttctctaaaa aggccagcca gcggcgactc caccagacgc
     1801 gtgtcgagcc gccctgcggt ccggcgctct gcgcacggat cccgccccgg acctgctgcg
     1861 gaagccgcgc agcataccgt ttcttctttt tgctcatgcc cagcctcccc atccgccttg
     1921 ctccggcgcg gcatcctcct cgtccaccac ctccgcatcg agtttgagca cctcgagcag
     1981 ggcgtcgtcc ggcagcccgg tgagaaactc ctcgccgctg accaccacct cgttggcgag
     2041 ccccgtcttg tctgcgatca gcgcgtcgat gcgttcctcc agtgtgccgt tgcagagcag
     2101 ggtgtgcacc atgacttcgc gggcctgccc gatccggaac acgcggtctg tggcctggtt
     2161 ctctacggcc ggattccacc aacggtcgaa atggaacaca tggttggcgc gcgtcagatt
     2221 caaccctgtg ccgcccgcct tgagcgagag cacgaaagcc tgcggccgcg tcccgttctg
     2281 gaacgcccgt atcatccggt cgcgttccgg ccgtgtcaag ccgccatgca ggaacggcgt
     2341 ttcgcgcgcg aaggtccggc ataggtggtg cgccagcaat ttacccatca cggcgtactg
     2401 ggtgaagacc agcgcgctct cgccacgcgc gaaaacctcc tcgagccgtt cgaccaaccg
     2461 ttccagtttg ccggaacgcc gcgccagatt ctccgattga ccgagaaagt gcgcgggatg
     2521 gttgcacacc tgtttgagcc gtgtcagcac cgcaaggatt agaccacgcc gctcgatccc
     2581 ctcgctcgcc tcggcatcgc gccgaaagga ttccagtacc tcttcgtaca gctcggcctg
     2641 ttcgcgtgtg agcggacaga agactttggc ttcgatcttg gcgggcaggt cggcgatcac
     2701 ccggcggtcg gtcttcaggc ggcgcaggat gaagggggct gtgacgcgtc gcagccgagc
     2761 gcgcgccccg ggatcggtcc cggcttggat cggccggaaa aacgtttcgc gaaacacggc
     2821 gcgccgaccc agtaggccgg ggttcagaaa atccatcacg gaccacagtt cgccgacatg
     2881 gttctccatg ggcgtacccg tgagcgcgat ccggtaatcg gcctgcagcg cgcgcgccgc
     2941 gcgcgcctgc cgtgtgtcgg ggttcttgat gttctgcgcc tcgtccagca cgatcccgga
     3001 ccagccgatc cgccgcagtg cggcgtaatc gcggtgcagc agcgcgtaac tggtcaccac
     3061 cacatccgcg tccatcgccg cgcgcgccag atcctcgcct ttggcccggc cgggcccgtg
     3121 gtgcagcagc aggcgcagct ccggcgcaaa ccgccgcgct tcgttcagcc agctgccgag
     3181 cactgtcatc ggaccggcca acagaaccgg acggcgttcg ccgcggtcct tttcgtgcag
     3241 caaaaaggcc aatgcctgca ccgtcttgcc gagccccatg tcatccgcga ggcaggctcc
     3301 gaacccccag gcacgcagaa aagccagcca gcggaatccg cggagttgat agggccgcaa
     3361 cacaccgcga aaacccgtag gcaccggcag ggactccatc ccgctcatcc cgcccgccag
     3421 accgtccagt aacggatcga gccatccccg gcctcgtgcc cgcgcgatct ccagaccgcc
     3481 cgctcccggt tccgtgccca atgccaacct caccgcttga cgcaccggca ccgtgtcggt
     3541 ccgttgttgt gcgcggagcg cttcgcgcac acgcttcaga tccagagtct gccattgtcc
     3601 ccgcaatgaa accagcggtg tttcggcgcg ctccagttgc acggcttcct ctttcgtcag
     3661 cggggtgccg ttgaacgtca ctgaccatgt gacggccaca cgcgcttccg aaagcgttcc
     3721 ggtcgtgggg tcttcgcctg taagcggggt cagatccgct tcgagcgcga gcgccgatgc
     3781 gtggcccgga cgccactgcg gcggcaggtt caccgcatac ccggcagcct ccagcactgc
     3841 cgcggccact gtcaggaaag cgtgggcctc ctcctcactc aacgcgcaac cgaattttcc
     3901 gccccgcgtt tcagcccgcg cgagcggcgg gaagagcaga gaggcctgac ccagcgacag
     3961 cagaaacgcc gcgtcccacg cagggggggg gacttctcct tccggcccct cttctgcaag
     4021 cacacgcaaa aaccaggccc cttctgggcc gtcaggcacc tccagcgtga aacgcggcac
     4081 ctttccgggc tcgccgctct ccaccggttg ccgccactgc cgtagcgctc cggccagagc
     4141 ctccaactcg gcttgcgtct tccagcgcac ctgccgcgtc tcgccgcgta aggccgcgaa
     4201 ccaggcatca tgcggactgt accacggacc gcgttcggca tgtgcgcggg aaagagtggt
     4261 caccacactc gcgcgcacca gccggtcgac caactcctcc agcgcctccc tcggcgagcc
     4321 gtttcctgcg gcaaaggccg ccgggggcag gcgcggcgcc agccgctcca gccgcttgcg
     4381 gtccgccgca tccagtgccg gaatccaacg cgcctcgtac accttctctg acgtgcggtg
     4441 caacgcgggc agaaagcgtc cgcgcgccac cagcgcgccc gcaaaccgaa acagggtggc
     4501 aaacccgagc aggctctcgc cgataaaaag ttcgtcggcc agacgcggct cgcgcacgag
     4561 ccccaacaac accaaagttg cgcgccacgg cagaaccaca gccgtcacgc gccagggatg
     4621 cgggaaatct ggcgcgtcct cttcccccgc tccgtcgaac accgcattgt tgaaaaaggg
     4681 ttgcgagggc acgggccgga acccgctgcc ccaagcgcgg ctcggcagaa acagttcgag
     4741 tgtggcaggc tgcggcgttt ccccggtccc cggcatgagg ggccgcagca gatggcacag
     4801 acgctccgct ccggcatcga agggggaaac cgccaccgca gaacgcggtc cgtccggcaa
     4861 accggacgcg caggcctcga cctcccccca aatatgcaga tgggacgcat gccagcaact
     4921 gtgcaggatg gtcatcaccg gtcaaacagt atacggctca tcctcctccg ccgcaagatc
     4981 cggccatgct gaaaagcggt tcaaaatcag ccgcagaaaa atcgccctac atcaggttga
     5041 tcgcatccac atccacggca agggttacgc cggatgccaa aagcggacgg cgcatgactt
     5101 cgcgcagcgg ccgtgtgatc tgccgcgtcg tgggtgcccg cagcaaaagc tgataccggt
     5161 acagcccttt ggctttggcg agcggtgcgg cacatactcc ggagatgcgc acggcggccg
     5221 cattccctgt tgttttggac agcgcgcgcg cgaaggcctc tgcgctgaag cgcacctttt
     5281 cttcggacgc gccccggaag gtcaggcata tcaggcgggc atacggtggg tagccgcccg
     5341 cctcgcgttc ggccagctca cgtgcggcga acggttcgaa accggcatcc gaggcggcgg
     5401 cgcagatagc gggatggccg ggcgaatagg tctggattaa cacctcgccg ggcagctcgg
     5461 cgcggcccgc gcggcccgat acctgcgcca gcagttggta ggttcgttca gcggcacgga
     5521 aatccggcat gtgcaggctc atgtccgccg cgagtacgcc gaccagcgtg acgttgggga
     5581 aatccagccc cttggcgatc atttgcgtgc cgatcaggat gtcggcacgg ccacttttga
     5641 attgcgcgag aatctcgtca tgactgcggc ggcgcgaggt gacgtccgca tccatgcgca
     5701 agatcttcgc ctgcgggaaa caggctgcca gcgcggtttc cgcgcgctgc gtgccgaaac
     5761 cggcgtagcg gaatgccgga tcaccgcaca gggggcagac gtcggggacg tgcttccatc
     5821 cgccgcagat atgacagcgc aggcatgcgt ccgcccggtg gtaagtgaag gtcacgctgc
     5881 atgcgtcgca ggctgcggca tacccgcagc gggggcagag cagcgcattg gcgtagccgc
     5941 gccggttgag gaagagaatg gtctgttcgc cacgttcgag acgaagccgg atcgcttcca
     6001 gcaggggctc ggagaagagt tgcgcatgac cggaacgggc ggtctccaga cgcatgtcca
     6061 caacctgtac gcgcggcata ggacgccccg ccacacgctg cgtgagatgt gccgcaacgt
     6121 atttgccctt ttcaacgttg agccaactct ccatcgcggg cgtggcgctg ccgagcacta
     6181 cggcgcaatg ctccatccac ccgcgcatca ccgccacgtc gcgcgcgttg tagcgcggca
     6241 cctcatcctg tttatacgag ggctcatgct cctcgtccac cacgatcagg ccgaggttac
     6301 gcaccggcgc gaaaatagcc gagcgcggcc ccaccaccac tgtggcctcg ccattgcgga
     6361 tgcggtgcca ctcgtcgaac cgttcaccat cgcccagtgc gctatgcagc accgcaatgc
     6421 gcttaccaaa ccgtgaggcg aaacgctgga cggtctgggg cgtgagggcg atttccggca
     6481 ccagcacgat cgcgcccagc ccgcgctgca acgcatgggc gatcgcctgc agatagactt
     6541 cggtcttgcc agaaccggtc acgccgaaga gcaacacggg cttgggcgca tgcgccgagt
     6601 cgggagcgcg ggcgtccccg cccgccggcg aaacgtctgc tggtctcccg gcggtctgac
     6661 tcgcgccagc atgtgcgggc ggggacgccc gcgctcccga ctcggcaccc tccccctgca
     6721 tcgcgcaaag gtctgtccgc tcacccgatt cgataccgcg gttggcttgg atatatgcga
     6781 ccacagaatg aaaatgattt ggatcgcgca cataccggtc gaaatactcg ggttgccaga
     6841 gcgccccttt ccgccctagg caccgattga tccgatgagc agaaaccgat ttccaatcat
     6901 gaacaatctg ggcgacggaa tccgcttgct cgacctcaat caacgcatga acatggttcg
     6961 gcatgatgga ccacgcaagc aatcgatatt tcgtgccgtc gccatgccga agcacctccg
     7021 aaacctctcg ggcaatctct ggctgtttca aatagcaagc tccgtgtcca gcatcttcat
     7081 aacgggcaat acgccggatc aactcttcgt tcgcggacgg aggtgtcggc acgatgttga
     7141 cgatgcccaa ttcgctcttc cacgcatcaa tgacagagga gggcaaactg tcaacaaggc
     7201 gaaacgtaat gaattgaaca agccccggat aatcaaaatg gggcacatat ccgcgtgagt
     7261 gccaatgcgc ctccgtcccc ggctcactat gaatcatgcc ggggaatttc aacgacgtcg
     7321 cgtttgtgcc gtcctttgtc aagtcgggcg tcaagtcggg agcgcgggcg tccccgcccg
     7381 ccggcgaaac gtctgctggt ctcccggcgg tctgactcgc gccagcatgt gcgggcgggg
     7441 acgcccgcgc tcccgactcg gggcggaccc ccgcatcaac cgccgcgatg atcagcttaa
     7501 gggcggaggc ttgttcgggc atgagtgcca aggggcgggt cggcagcacc ttgcggttgg
     7561 cgagaggatc gcgacgcacg gcgcggcgct cgatcaccgc cgcgcccttc gcggccagag
     7621 tgcggagcgt ctgcggggcg caggcgaact cgcgggtcag actgtggagc cagccgccat
     7681 ccacacggcg caggttttcc agcagttcgt tttggcgtga agtcagcgcg aactcccccg
     7741 ggcagggcgt gacatagaag cgctccttga ccttgatatc gcggttgcgc accgcagcgg
     7801 gcaccatgca gcgcagagcc gcctcgaacg gcgaacaggt ataatccgcc atccagcgcg
     7861 cgagcgcgat cagcgttggg gagaggaacg gcacgtcatc ctcgatagcg aggatcgggc
     7921 gcagccgccg ggtgcggatt ccatcggtct tgtcagaccc gccggacaaa ctgaagagtt
     7981 cgggaggtcc gggttccaac gcggtgtcct ccgcgatctc cacgacatag gcgtccagcg
     8041 tacgcttgcc gaagggcaca cgcacacgct gtcccggccg gaggcgcggc gcgagcgcgt
     8101 ccggaacggc gtaatcgaag agccggtccg ccgaaatctc tgtggccaca cgcgcgagca
     8161 tccgtggtct tcccgtgatc aaaaaagagg ggagcggctt gccgctcccc ctttttcctt
     8221 tccatgacaa aacgcgtaaa cctcagcctc cgggggtcac agaggttttc ggcgtcagat
     8281 acttgagctg ctgggcgtcc tgctgcagcg tggtgtcgaa ctgctggtta ttgtagatca
     8341 cgcgcatctc gcggtacttg ccgcggccct tcaagatcgc gccacccttg cggaccatga
     8401 cgaagccttt gttggagaag ggtttcggat ctgaggaaga gaagttcaac ttcgtgttgg
     8461 ccttgttgtc gttgtacttg gcggccagtg agctggcgtc gatgttccgc gtgacgagaa
     8521 tggggatgac gtcctccatc tcgtcgcgca gattgcaggc gatggtccac atgttgtact
     8581 cctcggagag attacgcttg ccgcaggagg ggatgccggc gccagccagt ttcgagtaat
     8641 cgaacccctc ggccacggga ctccagtccg tcgtgccggc attctcttcg tcgtacaggt
     8701 aggcgaaata cttggtgctg tccgtggaag ccgtttggcg gtcctgatcg tcctgggaga
     8761 gcgtgccggt ggccttggac atctcgtccg gccatacatt gccaagaccg agcggctcgc
     8821 gctccatgtt ggccgaggtg atcgccacga agatatcctt gccgcgtgtg ccgaccgccg
     8881 ccatgttggc ggccatcacc gcgttgccga tcgccgggaa aagcgccgcg gagaggatgc
     8941 cgaggatgcc gatcacgacc agcaactcga cgagggtgaa accgtctttt tgatgtcttg
     9001 tcatttcatg tctccttcat gcaggccggt ccgcggactg ccttgtctct gttgtataac
     9061 gccattgtgc caccagaagg ggggacacgt caagcgcgtg tttgggggat ggtgttttta
     9121 ttttccgcca acacgccctc gtcccttgtc atgatgccgg cgccgggtca gggggcgcgc
     9181 tcgcgcgccg cgcgttcggc cgcgcgctgg gccatccgcc gacgcgcctc ggccgcgatc
     9241 agctcgcgag cctttttccg ttgttcggcg gtcaggcggt cgcggatcac caccaactgc
     9301 cgggtggcga gtttggcctg ttcggtacgg tgccgcccga tgcgctcgat gatggccatg
     9361 atctcgtcca cgcttgcgcc gggttccgac agcacgttcc gggccagctc cccctggcgg
     9421 agcgcctcgc ggttgatcgt ctcctccagc cgttgggcct gggcttggat ctcctccagt
     9481 tcggctttga gctgttcagc ctgagcgccc cgtaacccca cttgttccat gaactcaggg
     9541 gacagaacgt gttttccgac ccaattgctg cggttgatgg tccggggcgg gcgcgggcgc
     9601 ccggctctcg ggggcggttg cgcctgaacc caacccgcaa cccacaccaa ggcgatcaat
     9661 ccgacccgta tactccgttt catgcctacc ctttcctgtg agaaacaggc aacctgtttc
     9721 tcgtcgcaac gttaccacga gaatgaaggg actaacgtcc cgccggatga tttattgccg
     9781 catccgtttt cacccggagc ggtgctccgg atagtcggct ttcttccttc tccgtcaagc
     9841 ccggtctttg gcgtgtcgcg ttctctccct cagaggtcga acagcgtgag ctggcccggg
     9901 atgtcgtcat cgttccggcg tcgactccgg cgggcgagtt tggggaggcc agactccagt
     9961 tcgccttctt ccagattcgc gaggatctca cgtgaacggt cgatcacctc atccggcatg
    10021 cccgccagcc gcgccacatg gatcccgtaa cttttgtcag ccgtgccggg agcgatacgc
    10081 cgcagaaaaa ccacggtgtc gccgtgctcc cgcacttgca cggtgtaatt tttcacgccg
    10141 ctcagggtca gcgccagatc ggtcagctcg tggtagtgcg tggcgaacag ggttttggcc
    10201 ttcacacgcg gcgtgttgtg caggtactcc gccacggccc aagcgatgct gatgccgtcg
    10261 aaggtgctgg tgccgcggcc gatctcgtcc agaacgatca ggctgcgctt cgtggcattg
    10321 ttaaggatgt tggcggcttc ctgcatctcc accatgaagg tgctccggcc gcgcgccaga
    10381 tcgtctccgg cgcccacgcg cgtgaagacg cggtcgagaa gaccgatacg cgcttcgcgc
    10441 gcggggacga aggaccccgt gtgcgccagg atcgcgatca gcgccacctg ccggatgtag
    10501 gtggatttgc ccgccatgtt cggcccggta atcaacatga tctggttgcg gtggccgtcg
    10561 agccgcgtgt cgttgggcac gaacggcccg gcgtcgggaa gttgttccac caccggatgg
    10621 cgtccgtcgc ggatttccag cgtgtcgtcc tctgtaattt cgggccggac atatccgagc
    10681 gcaagggcgc ggtcggcgaa aacggccagc acatccagat gcgccagggc gtcggcggtc
    10741 tcctgaatcg cggcggtctg ctccaccgct tgttcgcgga tgccgcagaa caactcgaac
    10801 tccagcgctg tggcgcggtc ctgcgcgccg aagatcttct gctcgtaggt cttgagttcg
    10861 ggtgtgatga accgctccgc gttgaccagc gtctggcgtc gctgatattc gggcggcacc
    10921 gcgccggcct gtcctttgga cacctcgatg tagaagccaa acaccttgtt gtgccgcact
    10981 ttgagggttt tgattccggt ccgctcctgc tccgaggcct ggtatgcggc aagccaggcg
    11041 tgcccctctg tggcgagggc gcgcagctcg tcgagttccg cgtgataccc cgctcggatg
    11101 atgccgccat ccttcagccc cgcggcgggt gtctctgcga tcgccgctgt gatgaggccg
    11161 accagttccg gcagggcgtg gaggcgctca gccagctctg ccagcagcgg atcgcctgcg
    11221 gccgccaccc gttcgcgcag accgggcacg ggatgcaggg agagggccag cgcgttgaga
    11281 tcccgcgcgt tgccgtgccc ggcaccgaga cgagcgatca gccgttcgag atcccgcacc
    11341 gtgccgaggc tttcgcgcag ctcgttcagc agcgtacgct gttttgtgag gcggtccact
    11401 gcatccagcc gacagcggat cgcctctgcc tgacgcagcg gccgcgtgag ccaggcgcgt
    11461 aacatccgtg cgcccatcgg ggtccgggtg tggtccagca cgcccagcag ggtcgcctgc
    11521 ggactctggc cgcgcggcgg aatcaagtcg agattcaggc aggtgttctc gtccaaggtc
    11581 aaaaactcgt gtgcgcggcg tacgcgcaac gtgcggatgt gccccacggg atggcgcagc
    11641 tcctcatgca cgtagtgcag cagcgcgccg acggggcaga cctgcgctgc acggccatcg
    11701 catccgtacc cctcgagcga gtgaaccccg aagtggcgaa gcagccggtc gtacgcacag
    11761 tcgtattcga gcagccaatc gtcaatctcg gtgaccatac ccggcgcatc cggcgcaaga
    11821 agagcggcca gacgcggatc gttgcgctga tcgcggccga ccaggcactc gctcggcgcg
    11881 gcgcggttga gttgctcggc cagcacggcg gcgtccggcg cttcctcgac cgtgagcgcg
    11941 ccggtcgaga gatccagcaa cgcgaggccg agccctttgc ccgcgtagac cgcggccagg
    12001 taattgttgc gcgcggcatc cagaatctcg tcctcggtca ccgtgccggg ggtgaccacg
    12061 cgcgtgacct cgcgccgcac gatacccttg gcttgggcgg gatcctccat ctgatcacag
    12121 atcgcggcct tgcgcccagc gcggatcagc ttggcgagat atgcgtcgag cgcatggtgg
    12181 ggcaccccgc acatgggtat gccggtgcgc cgggtgagtg ccacaccgag aatcggcgcg
    12241 gcctcgcgcg cgtcctcaaa aaacatctcg tagaaatccc cgaggcggaa caagagcagc
    12301 acatcctgcg gcagttccgc tttgattctg cggtactgcc gcatcatcgg ggtcaatttg
    12361 tcaccagcca tgtgaatccc actcctcacc ttctgcatca cacatgtgaa aaacaggctt
    12421 gagggaagtt tcgcaagtta cattctgccc gtcaacaccg gaagggcggg accggcttgg
    12481 cgggattgac aggcagggcg ggataggcga tgatccttga agacgataaa ggaaacacgt
    12541 tcagatgatt gcttctcccg tttatctggc ctccgtggtg gcgctggcgg ttgccgtttt
    12601 gctcattctg atcatcagac tgaagcagca accgtttgtt tcactgctcc tcgtgagcat
    12661 gggcaccgct ctggcggcgg gaatgccgcc ggagaaggtg atggcgtgca tcgagaaggg
    12721 gatgggcgga acgctcgggt tcatcgcggt ggtggtcggg ctgggcgcga tgttcgggca
    12781 gttgctggaa gtctcgggcg gtgccgagcg gctgacgctc acgctgttgc gcgttttcgg
    12841 caaggagcgc gcaacgtggg ccatggcgct ggccggattt ctggtggcga ttccggtttt
    12901 cttcgacgtg gggttcatca ttctcgtgcc gctggtctac accctttcgc gcgagaccaa
    12961 gaaatcgctg ctctattacg gtatcccgct gctggcgggt ctggcggtga cgcatgcgtt
    13021 tgtgccgccc acgccggggc cgatcgcggt ggcgcaactg gtgggggcgg atttgggacg
    13081 tgttattttc tacggcgttt tgatcgggct gccctgcgcg atcgtggcgg ggccggtttt
    13141 cgggacttgg atcgaccgcc gcattcacgc ggcggttccg gcctatatgg agacggaggc
    13201 cgcgaagaaa caatgggacg agaaggctct ccccggcttc ggccgcgtgt tcggtatcct
    13261 gcttctgcca ctgttcctga ttctgctgaa cacggtgacc caagccgtgc tggcggaagg
    13321 ggaatatgca acggttcgac acgtcttcgc gtttatcggg cacccttttt tcgcgcttct
    13381 ggcggccacg ctgctcgctt tcacctttct gggcacctgt cgcgggctga cgcgcgagaa
    13441 ggtgaacgag gtggcgacgt cggctttggc tccggcgggc ctgatcattt tggtgaccgg
    13501 cgcgggcggt gtgttcaaac aggtgctggt cgacagcggc gcgggcaaag ccttggccgg
    13561 gacgctagcc catacgggtc tgcatccggt ctggaccgcc tttctgatcg ccttgctcgt
    13621 gcgggccgtg gccggctcgg cgacggtcgc gatgctgacg gcgggcggga tcatggcgcc
    13681 actgctggcc ggacagcggg tggagcccgc gctgatcgtg atcgcaatcg cctcgggcgc
    13741 cacggcgctc tcgcacgtca acgacagcgg cttctggctg gtgaaccggt atttcggtct
    13801 gagcgtgaaa gaaacgctcc gctcgtggac cgtgatggag accctcatcg gcctgatcgg
    13861 cgcgaccttg gcgtggctgt tcagctatgt ggcctgaccg ccgcagcgtt ccgttacagc
    13921 aacccgcgct tgatcagctc ttccgcgatc tgcaccgcat tctgcgcggc acccttgcgg
    13981 atgttgtccc cgcagcacca gagcgccagc ccgttttcca cactcggatc ggcgcggatg
    14041 cgcccgacgt tcaccgtatc gccgccgccc gtgttcagcg gcatgggata ctgcgccgcc
    14101 gccagatcgt cgatcacccg tatgccggcg gcgttcgcca gaatggcgcg cgcctcctcc
    14161 ggtgccagcg gcgcactgaa ctcggcgtgc acggccaccg aatgcgcgta gaaaaccggc
    14221 acgcgcgcgc aagtcgccgc aatgcgcagg tcggggcagc ccagaatctt gcgcgcctcg
    14281 aaagtcagct tggcttcttc ggaggtgaac cccggcatct ccggcttggc accgccgatc
    14341 tggggcagca cgttgaaagc gatcggatga ggataggcgc acacctgcgg ctcgttaccc
    14401 gcggcatagg cgcggacctg ctgttccaac tcctcgacag cggcgcggcc cgtgccgctc
    14461 acggactggt aggtgactgc ggtcatgtgc cgcagcggca cacggcggtg cagtggcgca
    14521 atgcccatca gcgtaataat cgtactgcag ttggggttgg caatgaaccc ctgatgcgcg
    14581 gtcagggcct cgccgttgat ctcggggatc accagcggca cgcgcgcgtc catgcggaaa
    14641 tcgtcgccgt tgtcgatcac cacgcagccg cgccgaaccg cctcccaacc gaactgcttc
    14701 gacgcgcctt tggagccttc cgtcccggcg aacagcacga tgtccaaccc gtcgaaggcc
    14761 tcaggccggg cctcgatcac atggaaggtt tcgcccgcca cctcctcatc ccgctcacgg
    14821 gtggccatga tgcggatctc ccgtgcggga aacttccgct gccggagcac cttgatcatc
    14881 tccgtgccca ctgcgccgat gcccacgaga cctacgctgt actgcttcat tcgcaatccc
    14941 ttcgcttgct ttcaccggtc aatcagcgcg cgcatctccg gcgtgatctc gaggatctcg
    15001 aaatccttga accacatctt gccgtttgcg ccgccgtgga gttgcagggc caccttaccg
    15061 ctcttctcgc aaagcgggtc gaggatgtcc accgtgcgtt ggccgttgag gatcgagagg
    15121 atccggtcgc cgtacgccac cgtgcaggtc gtgttccatc ccttggggtt ccggacctgc
    15181 gcgatgaact cgtcattctg cgccacccaa ccgcgtccct tggtcttgtc gcccgcagta
    15241 tgccacaggg cggaatcctt ggcgtttccg gcgatctcgt tctggaaccc cttgagcagc
    15301 cacggcgtgt taacctcttc ggcgcggaaa tagagcgcgc tgttgccgcc gaagagttgg
    15361 taagtcacgc gcacgatgaa atcgctgtag ttccggtccg agaccacgag cccgtcccgc
    15421 ttctctccct ccttatgctg gccgatgaga atgccctctt catgattgaa cgaccaatcc
    15481 tccggcagca cgaaacgggc gccgcggagc gcgtaccggc catcctcacc cttgacaaag
    15541 aagggcgtcc accggctcgt gcccagatcc cggatacgga tgttgcgcca gcgggcgcag
    15601 ggtttgccgt ccggattttt gatcgcgtgc acttgcaggc cgatgaaccc ggcgagcgcg
    15661 gcgtcgtcaa aatagttcac gaccggcacg tcgttgagcc aagtgcggat cgagggcccg
    15721 acgcactgga tctccatccg gttccaatcc ccctgcttat aggcggcaac cgatttcgcg
    15781 gtcacctccg caggcgtgtt atccagccaa gtccgcttga atttgaaccc gcgccgcccc
    15841 tcgtcgtaaa tacgggccgt gaccggcgag cgccccgcga tttccgcctg atacccgtaa
    15901 accgaatcgg atccgtcttt gtgcttgcgc gccgcggaac ggaactgcac cccggagttg
    15961 ccgccctcga acttgaactc cagacgcagg atgaaatccc cgtactcttt ttcggtgcac
    16021 aagaacgaat tgcggcccgc gtcgccgaaa cagccgacga tcgcgccatc ctcaacccgg
    16081 taaggcgact tgccgccccg ctggatccag ccgttcagcg tcttgccgtc gaacagcgtc
    16141 acgaacccct cttcccccgc cggcgcgtcc gctgcgatcc ccgccgccag aataccggcc
    16201 attacccacc cgatccattg acgtttcatc ttgtcctccc ttggccgcaa gtatagtaca
    16261 ccccctgtgc gcttttccag cgcaattgcc tgacggaaag cggcttattc cgtcagcgcg
    16321 tacgacttag gcctgcttac tcgttgagct gaccctgata cacgcagcgc cggactttct
    16381 gaatcgaggt gcgttccagc ggttcgcggc gcaccttgat ttttcgcgga tgcttgtact
    16441 cggtcagaca ttcgcattgt gtgcgcaccc gttcgcggat caggcgttcg accgtctccc
    16501 aggggggctc cactccttcg tgggccgccg ccactgcctc gaggtcgggc accacgatcg
    16561 tgcccacacg ctcgccgggt attccgccca ccgtgtagcc gatcaccacc acgtcggcga
    16621 tcagcggatc ccgcgacagc acgttttcga cctcttcggg atagatgttt ttgccttcgc
    16681 ggttgacgat cagcgccttt ttgcgcccgc agatcgtcag gtacccttcg gcgtcctgca
    16741 cggccaggtc gccggtgcgc agccactcgc cgtcgaacgc ctcgcgggtg gcctcgaggt
    16801 ttttgaaata ccccttcatc acgatcgggc cgcggatctg cagttcaccc acgccctgcg
    16861 gatcgggatc cgcgatccgc acctcgatgt tgggcagttt tttcccgatc gtccccacac
    16921 gtgagttttt gctgttggtg atggaaacca ccggcgaaca ctccgtgagg ccgtacccct
    16981 ccacaatcgg cacacccaga cgccggaacc cgttgagcac gtacaaaggg cacggcgccc
    17041 cgccgacaat caggaaacgc aacctgccgc cgagtccttt cagcaccttt ttgcctaccc
    17101 agcaccccag ccccagccgg aatagaagtc gggcgatccg gttccgccgc agcttgtcgt
    17161 cgattttgtt gaacatcttt tcgaccagca gggggaccgc gatcaggatg gagggccgca
    17221 gtgtccggat gtcctcccct atgcttcgga gactcgcgat aaaagacatg ccgcagcccc
    17281 ggaccagcgg caccacaaaa ttcgccgtga acgaaaaagc gtggaacagc ggcaacacca
    17341 cgaggaagtc gtcctccgca gtgatgacgt cgccgatcgc gtcgaaacag ccgaaagcgt
    17401 cactgcaaaa attgaggtgc gtcagcatcg cgcctttggg cgtgcccgtg gtgcccgaag
    17461 tatagatgat ggacgcgacg tcctgggggt cgggccggtg gtccgcatac cagaggaggg
    17521 gttcattcgc cacgcccgcc cgcagcgtct cgtaatcgta acagggccgt cccgcgatcg
    17581 gcgcgaggcg ctcccccgcg ccatcggtca ccacaatgaa ccggacctgc ggcaggtcgg
    17641 tcagaatcac ttcgagcatg tcgagatggc gggtgtcggt caagaccgcc accgcctcgg
    17701 agtcgttcag gatgtacgac acctccgcag cccgcaactt gggatcgatc ggcaccaccg
    17761 ccacgcccgc gcctgcagtc gccagatagg tctcgatcca ctccggcccg ttctccatta
    17821 tcatcgcgac gcgctcggtg atcggcttca accccagacg cccgaacgcc tcggccatct
    17881 gccggacgcc cagcatcagg gattcatatg tacggctctg ccaagcctga tcccggtgat
    17941 agcgcaatgc gatcgccgcc ggccttctgg atgccgtatc ctcaaatatt tttcgaagag
    18001 tcatgtccgc ccagaatccg tccaagctgg ttgtgaaatg acacaatagc cggaagtgtc
    18061 ataaaagcca atcccaaaaa ggataaggtt tcagggtttc atggtttcat ccaaatcgga
    18121 atcggggtcg ggatcggaat cgctatcgga atcgggatcg tcgacccgcg acccgcgaca
    18181 ttcgacccgc gac
//
LOCUS       DAOSLE010000046        18187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3302_length_18187_cov_8.001710, whole genome shotgun sequence.
ACCESSION   DAOSLE010000046 DAOSLE010000000
VERSION     DAOSLE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18187
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3302_length_18187_cov_8.001710"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PK576_04790"
     CDS             complement(<1..491)
                     /locus_tag="PK576_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60942.1"
                     /translation="MNKNWIGMAGVAAAAVMMTGCATTPTVREVALDRAPITSKLEPQ
                     DVRRTVEKMAESLLSAPGVREAVGNTRPVLDVEPLKNRTTQHVDMVSITDSLRMQLLR
                     SGLFRFVDKSTSGTDIEFMDSQANLGLTDQRKAIKPGQQSAAQMYLTGALTEIKNQVG
                     RTV"
     gene            complement(521..943)
                     /locus_tag="PK576_04795"
     CDS             complement(521..943)
                     /locus_tag="PK576_04795"
                     /inference="COORDINATES: protein motif:HMM:NF018889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1425 domain-containing protein"
                     /protein_id="HOE60943.1"
                     /translation="MKRINGLLGAVALAAGLTALTGCVETAGTVVTTDHTTGSTVVLE
                     DSTRLRGQVQVMKVNYDEVNGLKRVHITLASTRHRRLRLHYRIIWFDANGMELDPDTK
                     TYRTLILEGRDAVTVTGVANSTAGVTSKLRVRDLEAAD"
     gene            complement(1015..2535)
                     /locus_tag="PK576_04800"
     CDS             complement(1015..2535)
                     /locus_tag="PK576_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60944.1"
                     /translation="MRNTIPCSAGCGALLLLVAAGCVSPNVSTDRALASLRAGDDPQA
                     LEWSEKLKRSFYSKDLGYAETGRVRMLSGDFHGSSTNFSVLIDKVIEDTESGPVIKMG
                     SVGANVMAGTITDDRTRSYHLPAYEFINALQYQLLNHIFLGAPDAAAVEARRAVFAQE
                     QIAEKYGKEVQEIQAQAADGKHAKASQTVDAQMQSMAPVLELSRSSFENGLTWYLCGL
                     LFEQDQDQANASLSYRKAWELMPGNPYIQKDFLRLLRTQDAEAYKGLLAQTGTDPATL
                     KRDATEIILILEEGFISQRQSMKVPLPVPGVNTVVSVDFPVYRDDLYAPSTIAIRGGG
                     RELGMIALALSVQSLAYRDLKEKIPGIVVRNATRVATRVAAQYGANQAGDLAKYSVMA
                     FNAVSAVINKADTRAWYTLPVVSHLYRGGVEPGEHVIELRNQANGLVTRVPVKVAEGE
                     TRLIWVADFGGNARAATASLNGKGAPATFRVCGSLLAGFPPVTLPGGPRTLGARQP"
     gene            complement(2606..3823)
                     /locus_tag="PK576_04805"
     CDS             complement(2606..3823)
                     /locus_tag="PK576_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOE60945.1"
                     /translation="MKNRAWLTFACAAAMAACAVRAQVVTEAEKLDPNASAELQAFKA
                     RVKAANPGMEVVSVEADVVKRAVLAPQPQPQNPKIAIFVKNQTRVPGMDDAVDGVRDR
                     ISAELAGAGLIVLDKAEIADGFNRFKITTAEERAGLVNGIFTGGSTVRVAQMLGCEYV
                     MLVSIIGASHTARMAGDKAVTVYTLRMTTKVNEAANGSSVYGSNWTNKLPVPGQYTDG
                     GDAMNYYEDLFDQWSTATGEEIAGKVATWRRAAPVDTALVEFSVSSTVDELIQGLESG
                     VRAPNELLDEMRRLVGGVTVELDGATVGSSPGTFKATPGLHQLRVSRQWMRPWQQTVN
                     IQQGATFRVALELSDAGLQRYRSLEGFRAAVAIGYAEAVMRKGVKINFDTAAWRDVGN
                     KGSEINLQQNAIQ"
     gene            complement(3859..4401)
                     /locus_tag="PK576_04810"
     CDS             complement(3859..4401)
                     /locus_tag="PK576_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60946.1"
                     /translation="MKVSKGMVGLLVLLGVGLVLPVRGEGQERAYTPFMFSLVTPLQV
                     PFRDFNVGGLRINAIYGECHDFDGLDISTVGRATGHGNGFQAALLANIVDGDGFGMQV
                     APVDYVTGEYVGLQIGVANFAEKAQALQIGFYNGANHIDGCQIGVINTTRTMMGFQLG
                     LINVIQDNDVPFMPILNCYF"
     gene            4588..5871
                     /locus_tag="PK576_04815"
     CDS             4588..5871
                     /locus_tag="PK576_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE60947.1"
                     /translation="MRILFILPGSGGSFYCQNCLRDTVLAAALQRRGHPVTLMPLYLP
                     ATAAMPAPEEVPVFYGAVTLYLRHRFAGLRRLPRSWFKPLDRWPVLRLAARFAGSTSA
                     SGLEDLTLSMLRGPDGHQADELNRVAEWVAGLPASERPSVILLSNALLAGLARTLSEA
                     AGCPVACWLQDEHVWLDAMAPQMRAAALRILRDDARAIDRFLAVSSSYAERMTDLLAL
                     DPARVRVVIPGVDPEAYAPADTTRRPATLGFLSRLSAEEGFDRFVDAFLLLRCDPRFT
                     GTRLAATGGPSADKRFLTRQRRKLADAGLLTDTEISPGQFSTDRFGFLSRLTLLSVPG
                     GAVPEALGYYALEALAAGVPVVLPAQGAFPELVSRAGCGTLLPDTRPESLAQAWAGLL
                     SDPERLRRDSEQGRRAAASIFSHDAAALALEQALA"
     gene            6183..7223
                     /gene="aroF"
                     /locus_tag="PK576_04820"
     CDS             6183..7223
                     /gene="aroF"
                     /locus_tag="PK576_04820"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE60948.1"
                     /translation="MIIVLKRHATETDIASVSDLVCSLRYQPRVIRGVEQTVIACIGD
                     ELSNRSLEVLNNLPAVESVFPVQKKFKLVSREYHPADSIVNVRNNLIGGGNIQVIAGP
                     CAIESFDQFRTAVADLSRCGIKIIRAMPFKPRTSPYDFQGLKLDGVKIMRDIKAEFDA
                     ALVSEVPGPAQIEILRDVADMFQIGARNAQNYDLLEHLAHAGKPVLLKRGMASTIEEW
                     LTAAEYLVVNGCPQVVLCERGLRSFDKTTRNLLDIGAVAVARNLSHLPVLVDPSHAAG
                     TRSLVMPLSRAGLAAGADGLIVEVHPDPVNAFSDATQQLESATFDRFLAELEPWIALA
                     RAQRALPNHQPA"
     gene            7231..8082
                     /locus_tag="PK576_04825"
     CDS             7231..8082
                     /locus_tag="PK576_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOE60949.1"
                     /translation="MTPHPLLYLGPEGTHSHEAALRYADAGARPTACLSHFDLFDRLC
                     RSRARVRPVAVVPVENSSEGPVTQTLDQLASHPGVTIAASFSLAVRHHLLTGPDTASL
                     KAVQRVYSHPQALGQCHAALRRLVPHAEPMPESSTAAAARRAASEPGSAALASADAAR
                     IYGLRVLRRNLQDSRTNTTRFFAVTAHRRTRPAARPAGEVRSLIHLVIGNRPGALLHA
                     LSPFDAAGVNLTFIQSRPLPNRPWEYGFFIEAVTDWRSPAAEAAWRLILALSESGRII
                     GTYPILP"
     gene            8090..8674
                     /locus_tag="PK576_04830"
     CDS             8090..8674
                     /locus_tag="PK576_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60950.1"
                     /translation="MNKLHQLRTIIASLDETLVNAFCGRSRFRMNLDLYNEPKRTLSI
                     SETATLFGATATIAGRVRILRPLYVNTLLPHLCAPGTDEDRRKGIAADANCMNALVQR
                     LNLSVHVAALKREEIPEPLRAPLMMRDPVALEEAITNHAVEAEVIARILDMSRGQHAC
                     APDVPERIAHLYAGWIIPLSRKIQVHDLLASHHR"
     gene            8762..10282
                     /locus_tag="PK576_04835"
     CDS             8762..10282
                     /locus_tag="PK576_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOE60951.1"
                     /translation="MRLDMFPAREAFTETLQTATVIPVGMKILADAETPVSVLSRFAN
                     ENANVFLFESAEGGERWGRYSFIGISARATIRIYREEVVCRQGITETRIPHRGDPLAV
                     LRDRMRPYTLAPMPGLPRFCGGLVGYFTYEMIHFFEPRVPNTLPQERPMAELFIPDTL
                     LIFDNAEHTLTVVALAFREDDADPDALYTAAVRRVHEVLDVLALPADVTMRLHKTPIR
                     MPQPAHPPERFQEQVEIVKRHIREGDIIQCVISQRFVGPAPDDVVSLYRAQRYVNPSP
                     YLFFLKSAECTLIGSSPETMIRLDNRTACLRPIAGTRPRGATEQEDRKYADALLQDEK
                     ERAEHLMLVDLGRHELGRVARTGTVQVTDLMVIERYSHVMHLVSNITADLEPACDAFD
                     LLRSSFPAGTLCGAPKIRAMEIITDLEEEPRGPYGGAVGYISFDGNMDFAICIRTAVV
                     EHGRLTVRAGAGIVADSDPETEHRETVNKAGAMARAIELLKRVQENELSPARPVKP"
     gene            10294..10881
                     /locus_tag="PK576_04840"
     CDS             10294..10881
                     /locus_tag="PK576_04840"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOE60952.1"
                     /translation="MILMIDNYDSFTYNLVQYLRELAADVTVYRNDALTVQDITTLAP
                     DAIVISPGPGRPEDAGVSCDVVRAFAGQIPILGVCLGHQTIGLVFGAAITYAKQVMHG
                     KTSEITCDGQGLFKGLGDRPFKAMRYHSLVIDPDTLPACLQITARSEDGEIMGIRHTT
                     HLLEGIQFHPESIMTPVGKRILRNFLKLATAPCPS"
     gene            10904..11926
                     /gene="trpD"
                     /locus_tag="PK576_04845"
     CDS             10904..11926
                     /gene="trpD"
                     /locus_tag="PK576_04845"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOE60953.1"
                     /translation="MNYSTLLKKLIARRDLTENEAFEAVTAILAGEMTEGQIGAFLAA
                     LAAKGETVDEVVAGARAMRTAAIRVQSLNPNTLDTVGTGGDGGMTFNISTTVAFVAAG
                     AGATVAKHGNRASSGKSGSADCLEQLGLNLDADPEIMEQALNEIGITFLFAPSFHKAM
                     RHAGPVRKQLGVRTLFNLLGPLTNPAGAPCQLIGVFAPDLTEIFAQALQKLGSRRVLV
                     VHGHDGMDEITLTAPTRCSELRDGRIKTYDIDPLALFDRFCTPSDLEGGTPADNAAIT
                     RALLSGGLHGPKRDIVLINAAASLLAGNRATDLADGIRKSAESIDSGAALDKLEQLIA
                     FSRQAM"
     assembly_gap    12188..12287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12288..12472
                     /locus_tag="PK576_04850"
     CDS             <12288..12472
                     /locus_tag="PK576_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60954.1"
                     /translation="GISGLTGLTGRGLARITGLTGRGLARITGLTGRDLAGISGLTGW
                     HKNRIKLCITHHKEKA"
     gene            12469..12912
                     /locus_tag="PK576_04855"
     CDS             12469..12912
                     /locus_tag="PK576_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOE60955.1"
                     /translation="MKSEIDTLTGLIIGCAMEVHKTLGPGFLESVYEKALAIELGLQG
                     LAYERQLPIHAQYKGHSVGDFVADLLVEKKVLVELKASQALHDRHGIQLVNYLTATGI
                     DDGLLLNFGADSLQFKRKRRTYTPPHSPSPQSRQSCQSCQSCNPG"
     assembly_gap    13084..13183
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13337..14812
                     /gene="trpC"
                     /locus_tag="PK576_04860"
     CDS             13337..14812
                     /gene="trpC"
                     /locus_tag="PK576_04860"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOE60956.1"
                     /translation="MTYLEKILASKRDEIARAKAHTTRAQVERLAAARRDTPRGFAAA
                     LNASGVRVIAELKRASPSAGDIRPDLDPADLARRYAEGGAAALSVLTEPAFFKSSADD
                     LRQAREAVALPVLRKDFILDPYQVYESVALNADAILLIVRIVDDDTLHALYTLACSLG
                     LDVLTEVFDEEDAARARALGATLVGINNRDLARFSTDTTRTARLAALFPPDVSIVALS
                     GIRTSDDIRQTLACGIRRFLIGESLVRQPDPVATLRAWTTLHPSFPLSPMLPNLKICG
                     ITEHATARFCAEMGVGALGAVFYAKSPRYVTPSQARALFEGLPGHVARVGVFANCLPD
                     AMLAAAREARLTTLQLHGQESLADIRSLLRSGYRVIRTLSATGDSLLKIAQEIPTEAG
                     ILVECGQGPLPGGNAAVWNWAEAAPLAAVRPFALAGGLTPDNITDALRGSRAAACDVS
                     SGVETRPGVKNHDAIQKLVNALRKPAVTSGIQPFWLPSPNS"
     gene            14840..16000
                     /locus_tag="PK576_04865"
     CDS             14840..16000
                     /locus_tag="PK576_04865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE60957.1"
                     /translation="MSHPKLENVNIESVVLLATPREMKRAVPQTSASIATVTRGRETI
                     ERVLEDEDHRLLAIVGPCSIHHLESAETYARKLKQLSDEIADTICVVMRVYFEKPRSV
                     LGWKGLINDPYMNDTFHIEEGIRMARHFLMRVAEIGLPAATEVLDTLMPQYIGDLISY
                     SVIGARTAEAQTHREIASGMSTPVGFKNSTDGSLVGAINGIHAAMGYHHFLGVTEDGL
                     PAVFSTTGNPYPHIVLRGGKRPNYDAASIAFCEAALRTARLPVQIIVDCSHGNSEKQP
                     DRQGMVLRDLLTQIEQGNRSLRGFMLESQLEWGSQPMPKDPGQLNPGVSVTDACIDWE
                     TTEVLLREARDRYKVVIEERYTSYAGHARTPPSFPVRRALRRFRLTNPPLFL"
     gene            16011..17216
                     /gene="trpB"
                     /locus_tag="PK576_04870"
     CDS             16011..17216
                     /gene="trpB"
                     /locus_tag="PK576_04870"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE60958.1"
                     /translation="MNFSTKPYPDATGHFGPYGGRYVGETLIAALTELESAWNDARND
                     PGFLDAFRAMLTHYAGRPTPLDFAGRLSDHIGGARIYLKREDCAHTGAHKINNVIGQG
                     LLARRMGKRRVIAETGAGQHGVATATIAARFGMGCTVFMGAEDVRRQAPNVLRMRLLG
                     AEVVPITSGTSTLKDAMNEALRYWVAEVRDTFYIIGTVSGPHPYPVMVRDLQRVIGDE
                     ARRQMLEQVGRLPDLLVACVGGGSNAMGLFYPFLDDPCEMLGAEAAGEGLDTPHHAAS
                     ISGGSPGALHGAMTYLLQNDEGQIQEAWSISAGLDYPGVGPEHALLHDLGRVRYEGVT
                     DREAVEAFKLLCQFEGIIPALESSHALAAALREAAARPRDQAVLVCLSGRGDKDLDHV
                     TAYMNEHNL"
     gene            17244..18056
                     /gene="trpA"
                     /locus_tag="PK576_04875"
     CDS             17244..18056
                     /gene="trpA"
                     /locus_tag="PK576_04875"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOE60959.1"
                     /translation="MNRIDQTFRTLSAARRKALVGYFTAGDPDFDTSLQILLAACAAG
                     VDVLEIGVPFSDPTCDGPTIQSASARALKSGMSLSDSIRLAAEVRAATTTPIVLFSYY
                     NPLFKYGLQRVADAVSAAGVDGMLVVDLPPEEAGPLSDAIRDKAIHLIRLAAPTTTPE
                     RIRKLVDGAGGFLYLITRLGVTGTGGLDYDAIAQQAAAVKAVTPLPVCLGFGISTGED
                     ARALAPLADGLIVGSAFVRLIGEAPPGADLPGRIADKVRELRIGMDASPKCR"
BASE COUNT         3295 a         6322 c         5085 g         3285 t
ORIGIN      
        1 tccacggtgc ggccgacctg attcttgatc tcggtcagcg cgccggtcag atacatctgc
       61 gccgcgctct gctgacccgg cttgatcgct ttcctttgat cggtcagacc gaggttggcc
      121 tgggaatcca tgaactcgat atccgtgccg gaggtcgatt tgtcgacaaa gcggaagagc
      181 cccgaacgca acagctgcat gcgcagcgaa tcggtgatgc tcaccatgtc cacatgctgc
      241 gtggtgcggt ttttgagcgg ctccacatcc agcaccggac gggtgttccc cactgcctcg
      301 cgcacgccgg gcgcgctcag caatgactct gccatcttct ccactgtgcg ccgcacatcc
      361 tgcggctcca gtttcgaagt gatcggcgca cggtcgagcg ccacctcgcg caccgtcggc
      421 gtggtcgcgc acccggtcat catcacggcc gccgctgcga cccctgccat cccaatccaa
      481 tttttgttca tccgtttgcc tcgctttctt ttttccgtaa tcaatccgcc gcttccagat
      541 cgcgcacgcg cagtttcgac gtcacgcctg ccgtcgagtt ggcgacaccg gtcacggtca
      601 ccgcgtcgcg gccctccaga atcaaggtgc ggtacgtctt ggtgtccgga tccagttcca
      661 tgccgttggc gtcgaaccag atgatgcggt aatgcaggcg cagcctgcgg tggcgggtcg
      721 aggccagcgt gatgtgcacg cgcttcaacc cgttcacctc gtcatagttg accttcatta
      781 cctgcacctg acccctgagc cgcgtgctgt cctccagcac caccgtgctg ccggtggtgt
      841 ggtccgtggt caccaccgtg ccagccgtct ccacgcatcc ggtcagcgcg gtcaggccgg
      901 ccgccagcgc gaccgctccg agcaacccgt ttatccgttt catcgttctc tcctttgttg
      961 tccttcgttt cgtgatccgg ggttttcccg gcctgtgaac actgcgccgc cttgtcaggg
     1021 ctgcctcgcc cccagggtcc gcggcccgcc cggcaacgtc accggcggga aacccgcgag
     1081 caggctgccg caaacccgga acgtggccgg cgcgcccttc ccgtttagcg atgccgtcgc
     1141 cgcccgcgcg ttaccgccga aatccgccac ccaaatcaga cgcgtctcac cctcagccac
     1201 cttaaccggc acgcgtgtga cgagcccgtt cgcctgattc cgcagttcga tcacatgctc
     1261 gcccggctcg accccgccgc ggtagagatg cgacaccacc ggcagggtgt accaagcccg
     1321 cgtgtcggct ttgttgatca ctgccgaaac cgcgttaaag gccatcacgc tgtattttgc
     1381 caagtcgccc gcctggttcg ctccatattg cgcagccacg cgggtggcca cacgcgtcgc
     1441 gttgcgcacc acgattccgg gaatcttttc cttgaggtcg cggtacgcca gcgattgcac
     1501 cgacagcgcc agcgcgatca ttcccaactc ccgcccgccg cctcgaatcg cgatcgtcga
     1561 cggagcatac agatcgtcgc ggtaaaccgg gaaatccacg gaaaccacgg tgttgacccc
     1621 cggcaccggc aaaggcactt tcatggactg gcgctgcgat ataaaccctt cctccaggat
     1681 caggatgatt tcggtagcgt ctcgcttcaa tgtggcaggg tccgtgccgg tctgcgccaa
     1741 aaggcctttg tacgcctcgg catcctgcgt gcgcaacagc cgcaagaaat ctttctgtat
     1801 gtaagggttg cccggcatca gctcccacgc cttgcggtat gagagcgacg cgttcgcctg
     1861 atcctgatcc tgctcgaaca gcaagccgca aagataccag gtcaggccgt tctcgaaaga
     1921 actgcggcta agctccagca ccggcgccat cgactgcatc tgggcgtcta ctgtttgact
     1981 ggctttggcg tgcttgccgt cagcggcttg ggcttgaatc tcctgcacct ctttcccgta
     2041 tttctcggcg atctgctcct gcgcgaaaac cgcccgccgc gcctccaccg ccgcagcgtc
     2101 cggcgcgccc agaaagatgt ggttgagaag ctggtactgc aaggcgttga tgaactcgta
     2161 ggccggcaaa tgatacgacc gcgtgcggtc gtccgtgatc gtgcccgcca tcacgttagc
     2221 ccccacgctg cccatcttga tcaccggacc gctctcggta tcctcgatga ccttgtcgat
     2281 cagaaccgag aagttggtgg aactgccgtg gaaatcaccg gacagcatcc gcacacgccc
     2341 cgtctcggca taaccgagat ccttgctgta aaaactcctc ttgagttttt ccgaccactc
     2401 caacgcctgc gggtcgtcac cggcccgcag actcgccaac gcccggtccg tgctgacatt
     2461 gggcgagacg cacccggccg caacgagcaa cagcaacgcg ccgcatccgg cggaacatgg
     2521 gattgtgttc ctcatgaacc ctccactcca tcagaacagc gtgcggcgca acgggaaccg
     2581 gctcgtcacg ccgcccgctc gtcaatcact ggatggcgtt ctgctgcagg ttgatctcac
     2641 tgcccttgtt ccccacgtca cgccaggccg ccgtgtcaaa gttgatcttc acccctttgc
     2701 gcatcaccgc ttcggcatag ccgatggcga ccgccgcgcg gaacccctcc agcgaacggt
     2761 accgctgcaa gcccgcatcc gacaactcga gcgccacccg gaaagtcgcg ccctgctgga
     2821 tgttcacggt ctgctgccac ggccgcatcc actgacgcga gacgcgcaac tggtgcaggc
     2881 cgggcgtggc cttgaacgtg cccggcgagg aacccacggt cgcaccgtcc aactctacgg
     2941 ttaccccgcc gaccaaccgc cgcatctcgt cgagcagttc gttcggcgcg cgcacaccgg
     3001 actccagacc ctgaatcaac tcgtccaccg tggaggagac gctgaactcg accagcgcgg
     3061 tgtcgaccgg cgcggcgcgg cgccacgtgg ccaccttccc ggcgatctcc tcacccgtgg
     3121 cggtcgacca ctggtcaaag agatcctcat aataattcat cgcgtccccg ccgtccgtgt
     3181 actgtccggg caccggcaac ttattggtcc agttggaacc gtaaacggat gacccgttgg
     3241 cagcctcgtt caccttggtg gtcatgcgca gcgtgtagac cgtgaccgct ttgtcgcccg
     3301 ccatgcgggc agtgtggctg gccccgatga tcgacaccag catgacgtac tcgcatccca
     3361 gcatctgggc cacgcgcaca gtggagccgc cggtgaaaat gccgttgacc aatcccgcgc
     3421 gctcctcggc agtcgtgatc ttgaaacggt tgaagccgtc cgcgatctcg gccttgtcca
     3481 agacgatcaa gcccgcgccg gccagctcgg ccgagatacg gtcccgcacc ccgtcgaccg
     3541 cgtcatccat accgggcaca cgcgtttgat tcttcacaaa gatcgcgatc ttagggttct
     3601 gcggctgcgg ctgcggcgcc agtaccgcgc gcttcaccac atccgcctcg accgagacca
     3661 cctccatgcc ggggttggcg gccttgaccc gtgctttgaa ggcctgcaat tcagccgagg
     3721 cgttggggtc cagtttctcc gcctcggtca ccacctgcgc gcggacggcg cacgcggcca
     3781 ttgccgcggc acacgcgaaa gtcagccatg ctctgttttt catatgcaac ctctcttccc
     3841 gccgtacggg gttcggattc agaaatagca gttaaggatg ggcatgaacg gcacatcgtt
     3901 gtcctggatc acgttgatga gcccgagttg gaagcccatc atcgtgcgcg tggtgttgat
     3961 cacgccgatc tgacatccgt cgatgtgatt cgcgccgttg taaaatccga tctgcagcgc
     4021 ctgagccttc tcggcgaagt tggccacgcc gatctgcagc ccgacgtact cgccggtcac
     4081 gtaatcaacc ggcgcgacct gcatcccgaa cccgtcgccg tccacgatgt tggccagcag
     4141 cgcggcctga aacccgttgc cgtgcccggt agcgcggccg accgtgctga tgtccagacc
     4201 gtcgaagtca tggcactcgc cgtaaatggc gttgatccgg aggccaccca cattgaaatc
     4261 ccggaacggc acctgcaacg gcgtgaccag actgaacatg aacggcgtgt acgcgcgctc
     4321 ctgcccctcg ccccttaccg gcaacaccag acccacgccc agcagcacca gtaaaccaac
     4381 catccctttc gataccttca tctcggcctc cttggcttga agacaccccc cgccgcaccc
     4441 gcctccgcct ttgcgtcccg tgcgcggtca aagaggtatc agatggttta gagtatgccc
     4501 caaaataaac gtccgttcaa tcagattctt gcctgcgggc gtttaaaacg tgaacgccgc
     4561 cgggcgtttt gctacgctgg cctccggatg cgcatccttt tcattcttcc gggatcgggc
     4621 ggttcgttct attgccagaa ctgcctacgc gacacggtct tggccgcagc gttgcaacgc
     4681 cgcggccatc ctgtgaccct tatgcccctc tacctgcccg ccaccgcggc gatgcccgcg
     4741 ccggaggagg tgcccgtctt ctacggtgcc gtgacgctct acctgcgcca ccgcttcgcc
     4801 ggtctgcgcc gtctgccgcg ctcctggttc aaaccgttgg atcgttggcc ggtcctgcgc
     4861 ctcgccgccc gcttcgcggg ttcgacctcc gcctccggac tggaggatct caccctctcg
     4921 atgctccgtg gaccggacgg gcaccaggcg gacgagctga accgggtggc ggagtgggtc
     4981 gcgggactac ccgcgtcgga acggccctcc gtgatcctgc tctccaacgc cttgctcgcc
     5041 gggctggccc ggaccttgag tgaggcggcg ggctgtccgg tggcctgctg gctgcaagat
     5101 gagcacgtgt ggctcgacgc catggcgccg cagatgcgtg ccgcagcatt acggatcctg
     5161 cgcgacgacg cgcgcgcgat cgaccgtttc ctcgcggtca gctcgtccta cgcggagcgc
     5221 atgaccgacc tgctcgccct ggatcccgcg cgggtgcgcg tggtcatacc gggcgtcgat
     5281 cccgaggctt acgcgccagc cgacaccacc cgccgcccgg ccaccctcgg cttcctctcg
     5341 cgcctctcgg ccgaagaggg cttcgatcgg ttcgtcgatg ccttcctgct gctgcggtgc
     5401 gacccgcgtt ttaccggaac gcgtctcgcc gcaacgggcg gaccctcggc cgacaaacgg
     5461 ttcctcaccc gccagcggcg gaagctcgcg gacgctggcc tgctgacgga caccgagatc
     5521 tcgcccggcc agttttccac cgaccggttc ggattcctgt cacggttgac cctcctctcc
     5581 gtgccgggcg gcgctgtccc cgaagcactg ggatattacg cgctggaggc cctagccgcc
     5641 ggtgtgccgg tggtgctgcc cgcgcagggc gcctttccgg aactggtctc ccgcgccgga
     5701 tgcggcacac tgctccccga cacgcgcccc gaatcgctcg cgcaagcctg ggccgggttg
     5761 ctgtccgacc cggaacggct gcgccgcgac tccgaacaag gcaggcgcgc ggccgcttcc
     5821 atcttctcgc acgacgccgc agcccttgcg ctcgaacaag ccctcgcctg aaccgcgcgg
     5881 caagtggccg gtaacaggtg ttcacccgtc gtctccggtc gcgcctgtcg attaaggatt
     5941 ttgtttttcg catagcagcc ccttgacaca tcccggggga agcggttaaa tacgcgcaca
     6001 actcatgaac agcacacgtt cctattttta ctttagctac tttaggttgc cggacacacg
     6061 gttcgggtcg acccggtagg cttgggtaat acgggaacag acttgaaaca aaccgcgcgt
     6121 ccggcaactt cggacacgcg gttgttgttt tttcaccccc ctcacggaaa acggaaacgc
     6181 tcatgatcat cgtactcaaa cgtcacgcga cggaaaccga catcgcctca gtcagcgatc
     6241 tggtctgttc cctccgctac cagccgcgcg tcatccgcgg cgttgaacaa acggtcatcg
     6301 cctgtatcgg ggatgaactg agcaaccgct cgctggaggt cctgaacaac ctgccggccg
     6361 tggaatcggt tttcccggtg cagaaaaaat tcaaactcgt gtcgcgcgaa taccatcccg
     6421 ccgactcgat tgtgaacgtc cggaacaacc tcatcggcgg cggaaacatt caagtgattg
     6481 caggcccctg cgccatcgag agcttcgacc agttccgcac cgccgtggca gaccttagcc
     6541 gctgcgggat caagatcatc cgcgccatgc cgttcaaacc gcgcacttcg ccctatgatt
     6601 ttcaggggct caagctcgac ggcgtgaaga tcatgcgcga catcaaggcc gaattcgacg
     6661 cagccctggt ttccgaagtc ccggggccgg cgcagatcga gattctgcgc gatgtcgccg
     6721 acatgttcca gatcggcgcg cgtaacgccc agaactacga tctgctggag catctggccc
     6781 acgcgggcaa accggtgctg cttaagcgcg gcatggcgag caccatcgag gagtggctca
     6841 ccgccgccga gtatctggtg gtcaacggct gcccgcaggt cgtgctctgc gaacgcgggt
     6901 tgcgctcatt cgacaagacc acgcgcaacc tgctcgacat cggagccgtg gccgtggccc
     6961 gcaacctctc gcacctcccc gtgctggtcg accccagcca tgccgcaggc acccgcagtc
     7021 tggtgatgcc cctgtcccgt gccggtctgg cggcgggcgc ggacggcctg atcgtcgagg
     7081 tccaccccga cccggtcaac gcattcagcg atgcgacaca gcaactcgaa tccgccacgt
     7141 tcgaccgctt cctcgccgaa ctcgaaccgt ggatcgcgtt ggcccgtgcg cagcgtgccc
     7201 tccccaacca ccaacccgcc tgagggtccg atgactccac accccctcct ttatctcggt
     7261 cccgaaggga cccactccca cgaagccgcc ctgcggtacg cggacgcggg agcccgcccc
     7321 actgcctgcc tgtcgcactt cgacctgttc gaccggttgt gccgttcgcg cgcgcgcgtc
     7381 cgtcccgtgg ccgtcgtgcc agtggaaaac tccagcgaag gtccggtcac ccagacgctc
     7441 gaccaactgg cgtcgcatcc cggggtgacc atcgcggcaa gcttctccct cgcggtccgg
     7501 caccacctgc tgaccggccc cgacacggcc tcgctgaaag cggttcaacg cgtgtacagc
     7561 catccgcagg ctttggggca gtgccacgct gcgctccgcc gacttgtgcc gcacgcggaa
     7621 ccgatgcccg agtcgagcac cgccgcggcc gcgcgccgtg cggccagcga accgggcagc
     7681 gccgcgctgg cctcggcgga cgccgcgcgg atctacggcc tgcgggtgct gcgccgcaac
     7741 ctgcaagaca gccgcacgaa caccacacgc tttttcgcgg tcaccgcgca caggcggacg
     7801 cgaccagcgg cacgtcccgc aggcgaggtc cggtccctga tccatctggt gatcggcaac
     7861 cggccgggcg cgctcctgca cgccctctcg ccgttcgacg ccgccggggt gaacctgacc
     7921 ttcatccaga gccgcccact gccgaaccgc ccctgggaat acggattttt tattgaagcc
     7981 gtcacggact ggcgctctcc cgctgcggag gccgcctggc gcctgatcct ggccctctcc
     8041 gaatcgggcc ggatcatcgg cacctatccg atcctgcctt gaggacacca tgaataaact
     8101 gcaccaactc cgcaccatca tcgcctccct cgacgaaact ctggtcaacg ccttttgcgg
     8161 acgctcgcgc ttccgcatga accttgacct gtacaacgag ccgaaacgga cgctcagcat
     8221 cagcgagacc gccacgctct tcggcgccac cgccaccatc gccggccgtg tgcgcatcct
     8281 gcgcccgctc tatgtcaaca ccctcctgcc gcacctgtgc gcaccgggta cggacgagga
     8341 ccggcgcaag gggatcgcgg cggacgccaa ctgcatgaac gctctggtgc agcggctcaa
     8401 tctctccgtg cacgtcgccg cgctgaaacg cgaggaaatc cccgaaccgc ttcgcgcacc
     8461 gctgatgatg cgcgaccccg tcgcgctgga ggaggcgatc accaaccacg cggtcgaggc
     8521 cgaggtcatc gcgcggatcc tcgacatgag ccgcggccag cacgcctgcg cacccgacgt
     8581 gccggagcgg atcgcccacc tctatgccgg atggatcatc ccgttgtcgc gtaaaatcca
     8641 ggttcatgac ctgctcgcct cacaccaccg gtgacccgga gatcgcggat tttttcccgt
     8701 tgacgccgga gcggcgtttc tatataaagt aacacctgaa caaatcaggc aggagtggat
     8761 gatgagactc gacatgtttc ccgcacgtga ggcgtttacg gaaacgctcc aaaccgccac
     8821 ggtgattccg gtgggcatga agattttggc agatgccgaa acgccggttt cggtgctctc
     8881 tcgtttcgcg aacgagaacg cgaatgtttt tttgtttgaa agcgcggagg gcggcgaacg
     8941 gtggggccgc tacagtttca tcggcatctc cgcccgcgcc accatccgca tctaccggga
     9001 ggaggtggtc tgccgccagg gtatcaccga aacgcgcatc ccccacaggg gtgacccgct
     9061 cgccgtgctg cgtgaccgta tgcggcccta cacgctcgcc ccgatgccgg gactgccgcg
     9121 gttctgcggc ggactggtcg gatacttcac gtatgagatg attcatttct tcgagccgcg
     9181 cgtccccaac accctgccgc aggaacgtcc gatggccgaa ctgttcatcc ccgacaccct
     9241 gctcatcttc gacaacgccg agcacacgct gacagtggtg gcgctcgctt tccgggaaga
     9301 tgatgcggat ccggacgccc tttacacggc cgctgtccgg cgcgtccacg aggtgctcga
     9361 tgtgttggcg cttcccgctg atgtcacgat gcgcctgcat aaaaccccga tccgcatgcc
     9421 gcaaccggca catccgccag aacggtttca ggaacaggtg gagatcgtga agcgccacat
     9481 ccgcgagggc gacatcatcc agtgcgtcat ctcccagcgt ttcgtgggac cggccccgga
     9541 tgatgtggtc agcctctacc gcgcccaacg gtatgtcaac ccctcgcctt acctcttttt
     9601 cctgaagtcg gcggagtgta cgctgatcgg ctcctcgccc gaaaccatga tccggctcga
     9661 caaccgcacc gcctgcctgc ggccgatcgc cggcacacgg ccgcgcggcg caaccgaaca
     9721 ggaggaccgt aaatatgccg acgctctcct gcaggacgag aaggagcgtg ccgaacacct
     9781 gatgctggtc gatctcggtc gccatgaact cggccgcgtg gcgcggaccg gcacggtgca
     9841 ggtcacggac ctcatggtca tcgagcgcta ctcgcacgtc atgcacctgg tctccaacat
     9901 cacggccgac ctcgagccgg cctgcgatgc gtttgatctg ctgcgctcct cgttccctgc
     9961 gggcacactc tgcggcgcac ccaaaatccg cgccatggag atcatcacgg atctcgagga
    10021 ggagccgcgc ggaccctatg ggggcgccgt gggatacatc tcgttcgacg gcaacatgga
    10081 cttcgcgatc tgcatccgga ccgccgtggt ggagcacgga cggctcacgg tccgcgcggg
    10141 cgccggaatc gtggccgact ccgatcccga gaccgaacac cgcgagaccg tgaacaaagc
    10201 gggcgcgatg gcccgcgcga tcgaattgct aaaacgcgtt caggagaatg aactctcccc
    10261 ggcccgcccc gtcaaaccct gaggaacccc gccatgattc tgatgatcga taattacgac
    10321 tcgttcacct acaatctggt ccaatatctg cgcgaactcg ccgccgacgt gaccgtgtac
    10381 cgcaacgacg ccctgaccgt ccaggacatc acaacgctcg cgcccgacgc catcgtgatc
    10441 tcgcccggac cgggccgccc cgaggatgcg ggcgtctcct gtgacgtggt ccgcgccttc
    10501 gcgggccaga tcccgattct gggggtctgc ctcggacatc agacgatcgg actggtcttc
    10561 ggtgccgcga tcacctacgc gaaacaggtg atgcacggca aaacctcaga aatcacctgc
    10621 gacggccaag ggctgttcaa aggactgggc gaccgtccct tcaaagccat gcgctaccac
    10681 tcgctggtga tcgaccccga caccctgccc gcctgtctcc aaatcaccgc gcgttcagag
    10741 gacggcgaga tcatggggat ccgtcacacc acccacttgc tggaggggat ccagttccac
    10801 cccgaatcca tcatgacgcc ggtcggcaag cgtatcctgc gtaacttcct caaactcgcc
    10861 accgccccct gtccttcctg acgtttccct catcctttcc atcatgaact actcaaccct
    10921 gctcaaaaag ctgatcgcgc gccgcgacct gaccgaaaac gaagcgttcg aggccgtcac
    10981 cgccattctg gctggcgaaa tgacggaggg ccagatcggc gctttcctcg ccgcccttgc
    11041 cgccaaaggc gagaccgtcg atgaagttgt tgccggcgcc cgcgcaatgc gcaccgccgc
    11101 gatccgtgtg caatccctca acccgaacac gctcgacacg gtcgggaccg gcggcgacgg
    11161 cggcatgact ttcaacatct ccaccacggt cgcctttgtg gccgccggcg cgggcgcgac
    11221 cgtggccaaa cacggcaacc gggccagttc cggcaagagc ggcagcgccg actgcctcga
    11281 acagctcggc ttgaatctcg acgccgaccc ggaaatcatg gagcaggccc tgaatgaaat
    11341 cggaatcacc tttctcttcg ctccttcgtt tcacaaggcg atgcgccacg ccggaccggt
    11401 ccgcaaacag ctcggcgtcc gtaccctttt caacctgctc ggaccgttga ccaacccggc
    11461 gggcgcaccc tgccaactca tcggcgtgtt cgcgccggat cttaccgaga ttttcgcgca
    11521 ggcgctgcag aaactcggct cgcgccgcgt actggtggtc cacgggcatg acggcatgga
    11581 cgaaatcacg ctcaccgcgc caacccgctg ctcggaactc cgggacggcc gcatcaaaac
    11641 gtacgacatc gatccgctcg ccctcttcga ccggttctgt accccttccg accttgaagg
    11701 cggaaccccc gccgacaatg ccgccatcac ccgcgccctt ctcagcggcg ggttgcacgg
    11761 acccaagcgc gacatcgtgc tgatcaatgc cgccgcctcg ctgctcgccg gtaaccgtgc
    11821 caccgacctc gccgacggga tcaggaagtc tgcggagtcc atcgattccg gcgcggcact
    11881 cgacaaactc gaacagctga tcgcattctc gcggcaggcg atgtaaaatg gatacatgag
    11941 atggcttttg tcaggatttc cggcagcttc acttcggcgg gaacggaaag gattacggga
    12001 ttggcaggat ggggtttagc caggattaca ggattgacag gattggcagg acggggttta
    12061 gccaggatta caggattgac gggattgacg ggacaggact tagccgggat tacaggattg
    12121 acgggattga cgggacgggg tttagccagg attacaggat tgacgggatt gacgggacag
    12181 gacttagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg ggatttcagg
    12301 attgacagga ttgacaggac ggggtttagc caggattaca ggattgacag gacggggttt
    12361 agccaggatt acaggattga caggacggga cttagccggg atttcaggat tgacgggatg
    12421 gcacaagaat cggataaaac tgtgcatcac gcatcacaag gagaaggcat gaaaagcgaa
    12481 attgatactc taactggttt aattatcgga tgtgcaatgg aagtccacaa gactctaggc
    12541 ccaggctttc ttgagtcagt ttacgaaaag gcactcgcga ttgaactggg gttacagggg
    12601 cttgcatatg agcgccaatt acccattcac gcgcagtata aaggccattc tgtgggtgat
    12661 ttcgttgcgg acctccttgt cgaaaagaaa gtccttgttg aattgaaagc cagtcaagcg
    12721 ctacatgatc ggcatggcat ccaacttgtt aattacttaa ccgccacggg cattgacgat
    12781 ggattgctgt taaatttcgg tgccgacagc ctgcagttca aacgtaagcg cagaacctac
    12841 acacctccgc actccccgtc ccctcaatcc cgtcaatcct gtcaatcctg tcaatcctgt
    12901 aatcctggct aaaccccacc ccctcaatcc cgtcaatccc gtaatcctgg ctaaacccca
    12961 ccccacacaa tcccgtcaat cctgtcaatc ccgtcaatcc tgtaatcctg gctaaacccc
    13021 accccacaca atcccgtcaa tcctgtaatc ctggctaaac cccaccccac acaatcccgt
    13081 caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctgtaat cctggctaaa
    13201 ccccacccca cacaatcccg tcaatcctgt caatcctgta atcctggcta aaccccaccc
    13261 cacacaatcc cgtcaatcct gtcaatcctg aaattctccc cgccgaagtg aagctgtcgg
    13321 aaatcctggc taaacaatga cctacctcga aaaaatcctg gcctcaaaac gtgacgaaat
    13381 cgcccgcgcc aaagcgcaca ccacgcgcgc tcaggtcgaa cggctcgccg ccgcgcgccg
    13441 tgataccccg cgcggctttg ccgccgcgct caatgcgtcc ggcgtgcgcg tgatcgcgga
    13501 actgaaacgc gcctcgccct cggccggcga catccgtccc gatctcgacc ccgctgacct
    13561 ggcgcggcgt tatgccgaag gcggcgcggc cgcgctctcg gttctgaccg aacccgcgtt
    13621 ttttaaaagt tcggccgatg accttcggca ggcccgtgaa gccgtcgcgc tcccggtgct
    13681 gcgcaaggat ttcatcctcg acccctatca agtgtacgag agcgtcgccc tgaacgcgga
    13741 cgccatcctc ctgatcgttc ggatcgtgga tgacgacacc ctccatgctc tttatacgtt
    13801 ggcctgctca ctcggcttgg atgtcttgac cgaggtgttt gacgaagagg acgctgcccg
    13861 cgcccgcgcc ttgggcgcga cgctggttgg gatcaacaac cgcgacctcg cgcgcttcag
    13921 caccgacacc acccgcaccg cgcgtcttgc cgcgctgttc ccgcccgatg tctccatcgt
    13981 ggccctgagc ggaatccgca catccgatga catccggcag accctcgcct gcggcattcg
    14041 ccgcttcctg atcggcgaga gtctcgtccg ccaacctgat cccgttgcca cgctccgtgc
    14101 ctggaccacc ctccacccct ccttccccct ttcccccatg ctccccaatc tcaaaatctg
    14161 cggcattacc gaacatgcga cagcccgatt ctgcgccgag atgggcgtgg gtgcgctggg
    14221 tgcggttttt tacgccaaaa gtccgcgcta cgtgaccccc tcccaggccc gggcgctttt
    14281 cgagggactg cccgggcacg ttgcgcgcgt aggcgtgttc gccaattgcc tgccggacgc
    14341 gatgctcgct gccgcgcgtg aagcgcggct caccacgctc caactgcacg gccaagagtc
    14401 tcttgccgac atccgttccc tgctgcgatc cggctaccgt gtaatccgga cgctttccgc
    14461 caccggcgat tctttgctga aaatcgcgca ggagatcccc acagaagccg gaattttggt
    14521 agaatgcggc caaggccccc ttcccggcgg caacgctgcc gtctggaact gggcggaagc
    14581 cgcgccgctg gcggccgtca ggcctttcgc gctggcgggc ggtttgacgc cggacaacat
    14641 caccgacgcg ctgcgcggtt cgcgcgcagc cgcatgtgat gtctcctccg gagtcgagac
    14701 ccgccccgga gtcaagaacc atgacgccat tcagaaactt gtcaacgcat tacgaaaacc
    14761 cgccgttacg agcggcatcc aacctttctg gctaccctcc ccaaactcct aactcctcct
    14821 tcatccttcc ttctttccca tgagccaccc caaactcgag aacgtcaata tcgaatcggt
    14881 cgtgctgctg gccacgccgc gcgaaatgaa acgcgcggta ccccagacct cggcgtcgat
    14941 cgccacagtg acccgcggcc gcgaaaccat tgaacgggtt ctggaagacg aggaccaccg
    15001 cctgctcgcg attgtcggcc cctgttcgat ccaccatctc gaaagcgccg agacgtacgc
    15061 gcgcaaactc aaacagttga gcgacgagat cgccgacacc atctgtgtgg tgatgcgcgt
    15121 ctacttcgaa aaaccgcgct cggtgctcgg atggaagggg ctcatcaacg acccctacat
    15181 gaatgacact ttccatatcg aggagggcat ccgcatggcg cgccatttcc tgatgcgcgt
    15241 cgccgagatc ggcctgcccg cggccaccga ggtgctcgac accctgatgc cgcaatacat
    15301 cggcgacctg atctcatact ccgtgatcgg cgcgcggacc gccgaagccc agacccatcg
    15361 cgaaatcgcc agcggcatgt ccacaccggt cggcttcaag aactcgaccg acggttctct
    15421 ggtcggcgcc atcaacggca tccatgccgc catggggtac caccacttcc tcggcgtcac
    15481 cgaagacggg ctgccagcgg tcttcagcac gaccggcaac ccctacccgc atatcgtgtt
    15541 gcgcggcggg aaacgcccca attacgatgc cgccagtatc gcgttttgcg aagcggcctt
    15601 gcgcacggcg cgcctgcccg tgcagatcat cgtggattgc agccacggga attccgaaaa
    15661 gcaacccgat cgccaaggca tggtgctgcg ggatctgctc acgcagatcg aacagggcaa
    15721 ccgctccctc cgagggttca tgctcgagag ccagctggag tggggcagcc aacccatgcc
    15781 caaggatccc gggcaactca acccgggcgt ctccgtcacc gatgcctgta tcgattggga
    15841 aaccacggaa gtcctgctgc gcgaagcccg cgaccgctac aaagtcgtga tcgaggaacg
    15901 gtacacctcc tacgccggcc acgcccgcac ccccccctcc ttccccgtga ggcgggcatt
    15961 gcgccgattc cgcttgacaa acccaccgtt gtttctataa aaagaaacgc atgaatttct
    16021 ccacaaaacc ctatcccgac gccacgggcc atttcggacc gtacggcggg cggtatgtcg
    16081 gcgaaacgct gatagccgcg ctcacggaat tggaaagcgc ctggaacgac gcccggaacg
    16141 accccggttt cctggacgcg ttccgcgcca tgctgaccca ctacgcgggt cgtcccacgc
    16201 cgcttgattt cgccgggcgt ctctcggacc acatcggtgg cgcgcgcatt tacctcaaac
    16261 gcgaggactg cgcccacacc ggcgcgcaca agatcaacaa cgtgatcggc cagggtctcc
    16321 tcgcccgccg catgggcaaa cgccgcgtga tcgcggagac cggcgctggc cagcacggcg
    16381 tggccactgc caccatcgcg gcacgtttcg gcatgggctg caccgtgttc atgggggccg
    16441 aggatgtccg ccgccaggcg cccaacgtgc tgcgcatgcg gttgctcggc gccgaggtgg
    16501 tcccgatcac gtccggcacc agtacgctga aggacgccat gaacgaggca ttgcgctact
    16561 gggtcgccga agtgcgcgac accttctaca tcatcggcac cgtgtcgggt ccgcacccct
    16621 accccgtgat ggtccgcgac ctccagcgcg tgatcggcga cgaagcccgc cgccagatgc
    16681 tcgaacaggt cggccgcctg cccgacctgc tggtcgcctg cgtgggaggg ggcagtaacg
    16741 ccatggggct tttctacccc ttcctcgatg atccctgcga aatgctcggc gcggaagccg
    16801 cgggcgaagg gctcgacacg ccgcaccacg cggcctcgat cagcggcggc tcgcccggcg
    16861 cgttgcacgg cgccatgacc tatctgctgc agaacgacga aggccaaatc caagaggcct
    16921 ggtcgatctc cgccggactg gactatccgg gcgtcggccc ggagcacgcc ctgctgcacg
    16981 accttggacg cgtgcgctac gagggcgtca ccgaccgcga ggcggtcgag gccttcaaac
    17041 tgctctgtca attcgagggc atcatccctg cgctcgaaag ttcgcacgca ctggccgccg
    17101 cactccgcga ggctgcggca cgcccgagag accaagccgt gttggtctgc ctctcgggac
    17161 gcggcgacaa agacctcgat cacgtcaccg cctacatgaa cgagcacaac ctctaattcc
    17221 tgacttccct ctcctccttg attatgaacc gcattgatca aaccttccga accctcagcg
    17281 ccgcacggcg caaagcgctg gtcggctatt tcacggctgg cgacccggat ttcgacacca
    17341 gcctccagat tctgctcgcg gcctgcgccg caggggtgga cgtgctggag atcggcgtac
    17401 cgttctccga tccgacctgc gacgggccca ccatccaatc ggcctcggcc cgcgcattga
    17461 aatccggcat gtcgctctcg gacagcatcc gcctcgcggc cgaggtgcgc gccgccacga
    17521 ccacgcccat cgtgctgttc agctactaca acccgctctt caaatacgga ctgcaacggg
    17581 ttgccgatgc ggtttccgcc gccggtgtgg acggcatgct ggttgtggat ctgccgccgg
    17641 aggaagccgg tcccctgagc gacgcgatcc gggacaaggc gatccacctg atccgcctgg
    17701 ccgcccccac caccactccc gagcgcatcc gcaagctggt cgacggcgcg ggcggattcc
    17761 tctatctcat cacccggctg ggcgtcacag gaaccggtgg actcgattat gacgccatcg
    17821 cacagcaagc cgccgccgtc aaggcggtca ctccgcttcc ggtctgcctg gggttcggca
    17881 tcagtaccgg cgaggacgcc cgggcgctcg caccgctcgc ggacggcctg atcgtgggtt
    17941 cggccttcgt gcgtctgatc ggcgaagcgc ctcccggcgc ggacctgccc ggccggatcg
    18001 ccgacaaagt ccgcgaattg cgcatcggca tggacgcatc cccaaagtgc cggtaaggac
    18061 gcatccccaa tgcgtccgcc aaatcgctat cgctatcgga gtcgctatcg ctatcggaat
    18121 cgctatcgga atcgattgtt taaccacaga acacaacccc tcaccccctc acagcctaac
    18181 cccctaa
//
LOCUS       DAOSLE010000047        18054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3332_length_18054_cov_8.474360, whole genome shotgun sequence.
ACCESSION   DAOSLE010000047 DAOSLE010000000
VERSION     DAOSLE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3332_length_18054_cov_8.474360"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            869..991
                     /locus_tag="PK576_04880"
     CDS             869..991
                     /locus_tag="PK576_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60960.1"
                     /translation="MLNWISAGSTVKALPLSVRGSRVSKLFTMLDQGHAKGITP"
     gene            1028..1183
                     /locus_tag="PK576_04885"
     CDS             1028..1183
                     /locus_tag="PK576_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60961.1"
                     /translation="MPFCGDYTEAAVWSGAQWTKHKRDQAKRDRADAVDRATLEALAR
                     ETRRTGL"
     gene            1219..3201
                     /locus_tag="PK576_04890"
     CDS             1219..3201
                     /locus_tag="PK576_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE60962.1"
                     /translation="MAVLLPVERKTMKARVFLALIYAVLSLGGITMVWPFLVMLASSL
                     TGPYDYYRHAPVVRAMWDRNDRFMRYLATCFPRFPSTVYPDAPTHWGSWTTVAREGVA
                     CRAWAAEQLKGLADPVTAERWRRMAEDYAAFNRTYEIANTVCHFDPRDVAGFVRRHYE
                     ERVRDEDPQAYNRMSPKARRQAALARLNAEWPVRYTSFFGIRMMAQQRAPLHHAGWDE
                     PIGDAKMAVYQTFKAMYREQAAARGVPMSRTRPFESLPLWSAYLRRALPEAADKWDGF
                     FPLPADAPDTVRRVWERFIREAYPRRLLRVRVTPERERAYRAYVRSVCRTPEAYAQLT
                     GRSLPPPDPSDTSEAAAFSGLPLPAYENSTLWRNFIPSVPLAELSVRSAEQAWQAFLL
                     ERYGSTEGVREAYGLPHARIEAARLPLREALAVTFARRGGRDFLDSMLANYRLVGEYL
                     FLRGQAFGNTLLLVVLSILATLTVNPLAAYALSRFGLRRAENILLFLLATMAFPAAVT
                     AIPGFLLIRDLGLLNTFAALVLPTLASGMSIFILKGFFDGLPRELYEAAAIDGAKEWQ
                     ILLRITLPMTTPILAVNALTAFVHAYNSWEWALLVCQKPSRWTLAVWMYQMSQQLSDQ
                     PWAVMAGFVLVSLPTALVFFACQKVILRGIVLPSMK"
     gene            complement(3679..6093)
                     /locus_tag="PK576_04895"
     CDS             complement(3679..6093)
                     /locus_tag="PK576_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60963.1"
                     /translation="MSLTSVLKTARLHARRWTAGWLLCGAATTGLAVTMTVAPNGNDT
                     NPGTAERPLASLTAARDAARQAGAGPHRIVVLPGTYYLEQPLTLDARDNGLTIEAAET
                     NRVTLYGGRRVTGWKREGDTLWVADLPGVKEGTWDFRALVVADRLAERARFPATNTLE
                     NLGTWNLRLLPAVAGHWERKPTKEEATTMPYKPEDLPPTLDIRNAEIRLYHMWSESLV
                     GVVSNDLQRHALILSPAPAWPPGALKRRKYLVFNTREGMTRPGQWYLDRTAGRLVYWP
                     LPGEDMTRLHVVAPTVERVIDLAGTARAPVRNITLRGLSVQATTAPLKLASFGAAAFD
                     GAVHLTRAHECVLEALQIRNVGGVGLKASDLNASRITYCDISDIGACGAVISAKDSLI
                     GHNHIHHLGRYYPAAAATSLRGDRIRIFRNEIHDAPYSGIISGGKENRIEENLIYRVM
                     RELHDGAAIYGNMNACVIRGNVVRDVVEVGKGFGASAYYLDEGARDCIVENNVAIGVP
                     MPTHNHITRNITVRNNVFIAERDMTVSFQRSVGCAFENNTLCFPGKLRLSHPNGIRSW
                     KGNLLFYEGLDQQGNPRRHTICDAMPHFDPPGRKTHAAVAVRVAHPPKLDGVITPDEW
                     PGDLQALNREPSRFTTGGAPTLAKFAYDDTHLYVAANVTMFGPAVVSTNAVWAKDDGV
                     EICLAGQTPDGKPATFVIRGFAGGALLSVTEAGAPAEAAARVGRESRFVVQSAPGPGN
                     RLFGRGWRGEWAIPLAALGIRPTPGLKVPFNMAAYYSEFDEWRCWEGTQAENWRLDQA
                     GMLEFK"
     gene            6260..6727
                     /locus_tag="PK576_04900"
     CDS             6260..6727
                     /locus_tag="PK576_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60964.1"
                     /translation="MRKRLCALMILMAGGAARGWEEPSVDLFAGWWVSPFRYGAYPYG
                     VFPWRCPDPVVGLGVPLYFRDGRAHDPYFADWGCYPYGWGYGQRVRLRLVEPRYAPAL
                     SDELSLPLPGSAPTELRDPERERAWEREFLRILGDRDEAAWRPDATNGCARSP"
     gene            7026..9488
                     /locus_tag="PK576_04905"
     CDS             7026..9488
                     /locus_tag="PK576_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE60965.1"
                     /translation="MGWLVAVLPLCAVTAAEWETARAALHDKLYPIAQAHAEKALAEG
                     EAPEIALLILAEALAAQDLPQELLRRLDAHPEWIQRAPQPEAFAYWRAWALMRLGQPL
                     EAARIAELAAAEPSSYADALRRLAARARLAAGDQAGALTLFATVDRTSTNRATRVENA
                     LDWALALEQAGRTEAALEVLKAQAEHAESGTAMSEGALLRGRLLFNLGQTSEATRIFN
                     QLAMNERATGSARVQALVEMSVYTLQEGKTNEALAYARSAYDRARQPESRLLAGFRLG
                     DLLSASPDTIDEAEKIIKALVREFPEHPCAMRAHLKLADALLQANRPERAADEYRIFL
                     ETYPPSSLHAEVLQGRGWALMRLGRYAEAGGMFQRASETATNLAEKAECIFKQGDALL
                     ADGRFAEASAAYAKMAGQFPQSPLAGRALYQSAECLERAGRRAEAGQQYRHVAQTFSD
                     REEAPRALLRYAAMQAEDNALEEAVKTYTDVLEAYPQPAVRADALMGRGKALYRTYRF
                     DAAMQDFASVAESTPARRDEARFLLTQCLYGLGRDREAHAAGTAFLLAFPESDFLPDM
                     MLWLGKTTFNSGAYEEASRFFLDYVTRWPAGRWADAALLWAARAAAAKMDFTGAVELV
                     TRLARAYPQSARLTEALRVQGDALMELARFDEAILLFDQILLKAPAGDEAKQALLRKA
                     DCLFALGAGNSLRYQEALEAYQTLLAENDLAPALTLQLHFKAGRCLEKLKRVDEAIDR
                     YYADVMLRYQDARSRGVWFDATGDSLFVRAAFNVADLYEQKGQPEQAVPVLRRVRDSG
                     MPGSEEAQRRIERLHQTKMGGI"
     gene            9485..10105
                     /locus_tag="PK576_04910"
     CDS             9485..10105
                     /locus_tag="PK576_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE60966.1"
                     /translation="MTILMQGGAVFWLIVLLGVAAVIVFFERMLNLWRAQIDYGDFLK
                     GVCNVLDNGNREEAMMICDETPGPVAAVVLTAIRHSQGTPEAIREAVDNTGRAEISRL
                     ERRLASLAVTCQIAPLLGLFAAILGGIRLLLTLTEQAPVIQSLDLSQSLMQALIAAAA
                     GVLVAATCHVMYALLMVRIERIVLDMEASASEIVAYLTHQNRPPVS"
     gene            10116..10631
                     /locus_tag="PK576_04915"
     CDS             10116..10631
                     /locus_tag="PK576_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60967.1"
                     /translation="MVSEKRESWSRQVRRHRTVYRFGGAWAQALLSAIPWVTTLLLAL
                     LLMLLHGRLAITPGVAFDLPTAPLREGSRARLTALMLQVTRGDLPSGEETLIFFDDDR
                     YVAQDPEQQLMLAGRLQDRMTLSVQRELVLLADKRVPHGEIMRFVQTARKAGAKRVLV
                     AEKPERGLERE"
     gene            10636..11355
                     /locus_tag="PK576_04920"
     CDS             10636..11355
                     /locus_tag="PK576_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60968.1"
                     /translation="MPDGFQRVPRFRARITHRPVLFWWQTCLVLAVFSLLWFELPPEA
                     VFYAPRAFAPLPAPHAAYVSLDPEYATEMFRKTVMGWMLGGVIGKDEGGMEFGLSVLS
                     DALPPPVLLEQGTRYPGVWQPLEVDLPAVQPSVLPAPGGAASCAPRVLAPPPEGVRVV
                     PDAALRAAGWEVAVPDGVLTVNEGHGRFYVETDADGHVAHVLVFPPHTPSTAALERVL
                     ARGRARGAARGIVEVHWRRTP"
     gene            11352..12509
                     /locus_tag="PK576_04925"
     CDS             11352..12509
                     /locus_tag="PK576_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HOE60969.1"
                     /translation="MMSRRPIPSRRPATAYVAVAAVIAAVAVIGWLARSMCAPQPVPS
                     AAQPAVRNAWRVVWRAALTAHANAPVPVDAEGWIVTDDAGGVTRLSDTGKPLWRVSFS
                     NQPFEASAAIHDRRVFAASTSGGVLALDAETGRALWSVTLDARFQHPPLLDAQGGGDA
                     AVMRLVSQSDGRLFCLRVRDGLTLWQSEPTNRCDGEPAAGPYGIAYGNCDGAIHVFDA
                     ADGRRKGSIPVGTEDQMAGGLLSLPDSRLVAGTRQGNLVVANARTFVCEARTRVSENE
                     AFLKPVRTQNGLIATGSEEGLVSLWFLRDTTLSQAGEYRFEQAIRDLAACGNRLFILT
                     GGALHIADTPDAAVCRVALGDEVYGLALRDCVTAACVADGMLVCVKGEAHE"
     gene            12502..13191
                     /locus_tag="PK576_04930"
     CDS             12502..13191
                     /locus_tag="PK576_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE60970.1"
                     /translation="MSNGADVLVTGVVRTFRPPGGVPVEVLRGADLTVTAGGSVAVTG
                     PSGSGKTTLLHLIGALDTPDAGEIRVNGQRLDALEEAARLRYRNREVGFVFQNHHLLP
                     QLTALENVLVPAWSGPDATARERAEHLLERVGLGDRMRHFPGHLSGGERQRVAVARAL
                     VMDPGLVLADEPTGALDQKNAAALIDLLLELNAERRATLIVVTHSELCARRMARRVKL
                     ENGTVREETGP"
     gene            13188..13763
                     /gene="pyrE"
                     /locus_tag="PK576_04935"
     CDS             13188..13763
                     /gene="pyrE"
                     /locus_tag="PK576_04935"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOE60971.1"
                     /translation="MTDKEILKTLEETGALLSGHFELRSGLHSNRFFQCANVLRYPRV
                     AGRLCEELTARARAAKALDGGVDGVISPALGGILVGHEVARALDVKCVFAEKQDGALV
                     MRRFTLKPGERYVVAEDVITRGGRVQETIDLVKAADAEVVAVLVMVDRSAGQAVFPYP
                     TFSLLHMAPEVWEPAACPLCARNEPFVHPGS"
     gene            complement(13813..15345)
                     /locus_tag="PK576_04940"
     CDS             complement(13813..15345)
                     /locus_tag="PK576_04940"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOE60972.1"
                     /translation="MKKMRKLGWVGAILLAGSVQAAVRMPKIFGDGMVLQQQVAVPVW
                     GWAAPGERVTVTFAGQSKETAAKADGTWSLKLDALQASSEPARLEVRGANALTFSDVV
                     VGEVWFCSGQSNMEWTMRSVDHAEKEIAAANLPLIRHFKAPKRVSGLPLDDVNAVWEK
                     TSPAGIGDESAVAFLFGRRLHQVLNVPIGLINCSWGGTRIEPWTPLCGFEGIPALEGI
                     LRNAQKRVEQVSPGSTSYKATHQTYFTRLQEWIAESEKRLAANQAVTAMPEYPSVFSF
                     RNQDPTVLFNGMVAGLVPYAIRGAIWYQGCSNNGEGMLYLEKTKALVKGWRQVWGQGD
                     FPYYLVQLAPYTYGGNPENLPGIWEAQAAVPAAIPNTGYTVINDIGNIRDIHPRNKQD
                     VGLRLANQALNRTYGRSEIAWAGPVYRSFAIEGQTVRVTFDHAEGLKTRDGQPPTWFE
                     ICGEDGRFVKADAKIDGTSVILSASSVSAPKAMRFAWNQIAEPNLVNGIGLPAGAFRC
                     GK"
     gene            complement(15427..17649)
                     /locus_tag="PK576_04945"
     CDS             complement(15427..17649)
                     /locus_tag="PK576_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE60973.1"
                     /translation="MREPGRFGWMVGLMAAMAVAGASAQTVFHVDAQRGQDTQDGSAA
                     RPFASLKRAQTAIRELKTNQTFPEKGVTVEVSGTFVMPEETVSFEESDSGIGPNAPVI
                     VRAAKAGALFLGGRRLPAEGFKPVSDAATLARLPEAVRGKVVAFDLKTIGVTRLAPLP
                     DKFHGWREMELFSNGKAMRLARWPNTGWAQIAKVIDRGVKPIDRATGEWEFGVRGGIF
                     EYAEDAPARWDVSKGIWMNGFWCHDWANETLRIGAIDKEKKQITSAAIHTYGIGNSSK
                     WHTAKRRYYVFNLLEELDMPGEWYVDREACLLYLYPTDSGLGDVVLTDQKKPLFRLNK
                     VRYVRLDGLTFRYNTGLAVSVNGCEEVRLENLRVSDITLDGIHITGGRACGLNRCEVY
                     GVGGTCVSVSGGDRKSLTACDHFVTNCRLHHSGRLQRTQGKCLNFNGVGIRIAQNLIY
                     DAPYIAVTYGGNENLFEYNEVHSAMMESGDGGGLYTGRNWCSQGNVIRHNYLHHFGQP
                     GVDWQKARGLNPDYEPLKESVMVMGVYLDDCDSGDTICDNIFYRAGWAAFVGGGRYNT
                     VRNNLFIECTSALHLDNRGLKRARPGEGTKDGWDLLAKLQAVKWQESPWKDKYPHLVN
                     IMEDEPKLPLHNVFRENVAVNCKLFLNANNVVRTGVVHRLTFENNLSFGPIDKRDVMA
                     FPQTDEGKRRVTFLETPLPDSVDPDTNGFRIQDSGEFKRLAPWFKRISLERIGPEKKN
                     "
     gene            17809..17931
                     /locus_tag="PK576_04950"
                     /pseudo
     CDS             17809..17931
                     /locus_tag="PK576_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3071 a         5561 c         5961 g         3461 t
ORIGIN      
        1 cccgagcagt gccatcccgt gagttcgtgg ttaaataatc gatcccgata gcgatcccga
       61 ccccgatacc gatttggatg aaacctgaaa ccttgaaacc ctgaaacctt gaaaccctaa
      121 aaccttgaaa ccctaaaacc ctgaaaccca agaaagtgtc ccgtggtgaa acagtcgatc
      181 ccgatcccga tagcgatccc gaccccgatc ccgatttaga tgaaaccttg aaaccctgaa
      241 accctaaaac ggaaccttca tgaaaagcag gggcatcttg gtgttcaaag ggcaatgcgg
      301 tttgtggccg gattcatagc gactagcggg agtcgggaac gtgggcgtcc cccgtccgca
      361 acagcggatc agtcggattg gaggcgttcc tgtcgcatgt ggtctgtcgc gggtcgtagg
      421 gcgacgttca cgaaaacgcg aacggggatg attacgggga agcgcttccg atagcgataa
      481 aaccggaaac cgggttgcgg gcgaggacgt ccgcgctctc gcctttctgc ccgtccttga
      541 tttcatgaag tttatgggtt tcatggtttt ttagatctcg ttggagcatg cgacgggctg
      601 tccacttctc atgatatgtg ggcagaggag gggcgaggtc tgaggtcagc gcaaagacgg
      661 agagagaacg tctggactgc cagaaatccc ttgcctgatt ggaaaaaaag ggggaacctg
      721 ttttcgacaa caatgacgtg ccgaacaccc ataacctatt cggcttcgcc agacctcacc
      781 cgtgttggaa ttacgggttt ttcggcttcg ccaagacgga cggtgtttgt tatagtgaat
      841 agcatacagg ggagatgcgg atcacgcggt gctgaactgg atctcggccg gatcgaccgt
      901 taaggcgttg cccctatcgg tccggggatc gcgcgtcagc aagctcttca caatgctgga
      961 tcagggccac gccaagggga tcacgcctta ggagaaggcg ctccttgcgc tctggaccga
     1021 tctcggtgtg cccttttgcg gtgactacac cgaggctgcg gtttggagtg gggcgcagtg
     1081 gacgaagcac aaacgggatc aagctaagcg cgaccgggct gatgccgtgg atcgcgccac
     1141 gttggaagcg ctggccagag agacgcgccg cacgggactg taagcggcgg atcggctcag
     1201 aaaagagggg ggaatcgcat ggcggttctg ttgcccgtag aacggaaaac gatgaaggcg
     1261 cgggtctttc tggccctgat ctacgccgtg ctgtcgctcg gcggaatcac catggtctgg
     1321 cctttcctgg ttatgttggc ctcttccctt accggcccgt acgactatta ccgccatgcc
     1381 cctgtagtac gcgcgatgtg ggaccgcaat gaccgcttca tgcgttatct cgccacttgt
     1441 ttcccgcgtt tcccctccac ggtttatccc gacgccccga cgcattgggg aagttggacc
     1501 acggtggcgc gcgaaggcgt ggcgtgccgc gcctgggctg cggaacagct caagggactg
     1561 gcggatccgg ttacggccga acggtggcgg cgcatggcgg aggattacgc cgccttcaac
     1621 cgtacctatg aaatcgccaa caccgtgtgc cacttcgatc cgcgcgatgt ggccgggttt
     1681 gtgcggcgtc attacgagga gcgggtgcgc gacgaagatc cccaagccta caaccggatg
     1741 tcgcccaagg cccggcggca ggccgcgctc gcgcggctga atgccgagtg gccggtgcgc
     1801 tacacctctt tcttcggcat ccgcatgatg gcgcagcagc gcgcccccct gcaccatgcg
     1861 ggctgggacg agccgatcgg ggatgccaaa atggccgttt accagacatt caaggcgatg
     1921 taccgcgaac aggccgcagc gcgcggggtg cccatgagcc ggacacggcc ctttgagtcg
     1981 ctgccgctgt ggtccgccta cctgcggcgc gccctgccgg aggctgcgga taagtgggac
     2041 ggttttttcc ccctgcctgc cgacgcgccg gacacggtgc gccgggtgtg ggagcggttc
     2101 atccgggagg cctacccgcg acgactgttg cgcgtgcggg tgaccccgga acgcgaacgc
     2161 gcctaccggg cttacgtacg ctccgtgtgc cgcacgcccg aagcttacgc gcaactgacg
     2221 ggccggtcgc tgccgccgcc cgatccgtcc gacacttcgg aggctgcggc gttctcgggc
     2281 ctgccgctgc ctgcttatga gaacagtacg ctgtggcgta atttcatccc ctcggtgccg
     2341 ctcgcggagc tgagtgtgcg gagtgcggaa caggcgtggc aggcgtttct gctggagcgg
     2401 tacggttcaa cggaaggcgt ccgggaggcc tacggtctcc cccacgcgcg gatcgaggcg
     2461 gcgcggttgc cgttgcgcga ggcgctggcc gtcaccttcg cgcgccgcgg cgggcgtgat
     2521 tttctggaca gcatgctggc caattaccgt ctggtggggg agtatctttt tttgcgcggg
     2581 caggccttcg gcaacacgct gctgctggtc gtgctttcca tactcgccac gctgacggtg
     2641 aatccgttgg cggcgtacgc gctctcgcgt ttcggtctgc gccgggccga gaacatcctg
     2701 ctcttcctgc tggccaccat ggccttcccc gccgcagtca cggccatccc cggctttctg
     2761 ctgatccgcg atctgggcct gctcaacacc tttgcggcgc tggtgctgcc cactctggcc
     2821 agcggcatgt cgatcttcat cctgaaaggt ttttttgacg gcctgccgcg cgaactgtat
     2881 gaggccgcag ccattgacgg tgccaaagag tggcagatcc ttttacgcat caccctgccg
     2941 atgaccacac cgatcctggc ggttaacgcg ctgacagcgt ttgttcatgc ctacaacagt
     3001 tgggagtggg cgttactggt ctgtcagaaa ccgagccgtt ggaccctggc ggtgtggatg
     3061 taccagatga gccagcaact cagcgaccag ccctgggccg tgatggcagg gttcgttctg
     3121 gtctctctcc ctacggcgtt ggtgttcttc gcctgccaga aggtgatcct gcgcggaatt
     3181 gtgctgccga gcatgaagtg aaatgcggtc gggagtatgc agccgtaaaa tccgtggcgc
     3241 gaccggcgcg agaagcgttt cgacctgtcc cgaagcgggg atcgcggggg tccgaaaaaa
     3301 acaccgcccc ccccttgaca ctttggggtg cgtcatatac tatacacccc gttcagacaa
     3361 cctcccccag gttgatgaac gatgcggcct agcgcgacgc ttcccccgcg atcgcgaata
     3421 caggttcccc cctgtagggc gcatctcaat tatatatgtc ctccctggcg gcggataaaa
     3481 gtaaaaaact tttatccgcc gttctttttt gccgaaaagg atcgggacga aacaggcgtc
     3541 ccccgcctgc tgacaaggcg gtttcaccga aaccgccgcc tcatcaaaaa acgccgcggg
     3601 tcgaatgtcg cgggccgcag gtcgacgtcc actgggacac ggaacacgga cgggggaaac
     3661 aatccgatcc cgacagggtt atttgaactc cagcatccct gcctgatcga gacgccagtt
     3721 ctcggcctgc gtaccctccc agcagcgcca ttcatcgaac tcgctataat acgcggccat
     3781 gttgaagggc accttcaggc cgggtgtggg ccggatgccg agcgcggcga ggggtatcgc
     3841 ccattcgccg cgccagccgc gtccgaagag ccggttgccc ggtcccggcg cggactgcac
     3901 cacgaaacgc gactcgcgtc ctacccgcgc cgcagcttcg gcaggcgcgc ccgcttccgt
     3961 gacgctcaac agcgcgcctc ccgcgaatcc gcgaatcacg aaagtggcgg gctttccgtc
     4021 aggggtctgt cccgcaaggc agatctccac accgtcatcc ttggcccaga ccgcatttgt
     4081 gctcacgacc gccgggccga acatggtcac gttcgcggcc acgtagaggt gggtgtcgtc
     4141 ataggcgaac tttgcgagcg tgggcgctcc gccggtggtg aaccgtgacg gctcgcggtt
     4201 cagggcctgc aagtccccgg gccattcatc cggcgtgatc accccgtcca gcttcggcgg
     4261 atgcgcgacg cgcacggcga ccgcggcatg agttttgcgg cccggcgggt cgaaatgcgg
     4321 catcgcgtcg cagatcgtgt ggcgccgggg gttaccctgc tgatccaagc cctcataaaa
     4381 gagcaggttg cctttccagc ttcggatgcc gttcggatgc gatagacgga gctttcccgg
     4441 gaaacagagg gtgttgttct caaaggcgca ccccaccgaa cgctggaacg agacggtcat
     4501 gtcccgttcc gcgataaaga cattgttgcg caccgtgatg ttgcgggtga tgtggttgtg
     4561 ggtcggcatc gggacaccga tggcgacatt gttttccacg atgcagtcgc gcgcgccctc
     4621 gtccagatag taggccgagg cgccgaatcc cttgccgacc tccaccacat cgcgtaccac
     4681 gttaccgcgg atgacgcatg cgttcatgtt gccgtaaatg gcggcaccgt catgcagttc
     4741 gcgcatcacc cggtaaatca gattctcctc gatccggttt tccttaccgc cgctgatgat
     4801 cccggagtag ggggcgtcgt ggatttcgtt acggaaaata cggatccggt caccgcgtaa
     4861 cgaggtggcc gccgcagcgg gatagtaacg cccgagatgg tggatgtggt tgtgtccgat
     4921 gagggagtcc ttggcgctga tgaccgcgcc gcaagcaccg atatccgaga tgtcgcagta
     4981 ggtgatgcgg ctggcgttca gatcggaagc cttgaggccg acgcccccga cgttgcggat
     5041 ttgcagcgct tccaacacgc actcgtgggc gcgggtgaga tgcacggcgc cgtcgaacgc
     5101 cgccgcgccg aaactggcga gtttaagcgg ggcggtggtg gcctgcacgg agagtccgcg
     5161 caacgtgatg ttccggaccg gggcccgggc cgtgccagcg aggtcgatca cccgttcgac
     5221 ggtcggcgcc accacatgga ggcgggtcat gtcctcgccg ggcagcggcc agtagacgag
     5281 ccgtccggca gtgcggtcga gataccattg ccccggacgg gtcatccctt cgcgggtgtt
     5341 gaaaaccaga tactttctgc gtttgagcgc acccggcggc caagcgggcg caggcgaaag
     5401 gatcagcgca tggcgctgca gatcattgct gaccaccccg accagcgatt cggaccacat
     5461 gtggtagagg cggatttccg cattgcggat gtcgagcgtg ggcggcaggt cctcgggctt
     5521 gtaaggcatg gtcgtcgcct cttccttcgt gggtttacgt tcccagtggc cggccacggc
     5581 gggcagcagg cgaagattcc aggtgccgag gttctccagg gtgttcgtgg cgggaaagcg
     5641 cgcgcgttca gccaggcggt ctgccacgac cagcgcgcgg aaatcccatg tgccctcttt
     5701 cacccccggc agatcggcaa cccagagcgt gtcgccctcc cgcttccagc cggttacccg
     5761 gcgtccaccg tacaacgtca cgcggttggt ctccgccgcc tcgatcgtga gtccgttgtc
     5821 gcgcgcgtcc aaggtcaacg gctgttcgag atagtacgtg ccgggcaaca ccacgatccg
     5881 gtggggacca gcgccggctt ggcgggccgc gtcccgtgcg gcggtaagcg aggccagcgg
     5941 ccgctctgct gtgccggggt tggtgtcgtt cccgttcggg gccacggtca tcgtaacggc
     6001 caatccggtg gttgccgcac cgcacaggag ccagcctgcg gtccacctcc gcgcgtgcag
     6061 ccgggcggtc ttgagaacgg atgtcagact catgatcgtt tcctccagaa cgggttaatg
     6121 acggaggagg ggcgcgccgg cctcgaccgg acaggctctc gccctttccc ccgaatgcaa
     6181 ccattatgtt gcggttcact cggctagtca atgcgcaaac accctttttt gtggccggga
     6241 ggtcgtgtat actgtgtcca tgagaaaaag gctgtgcgca ttgatgattc tgatggcggg
     6301 aggggccgcg cggggttggg aagagccgtc cgtcgatctc tttgcaggct ggtgggtgtc
     6361 gccgttccgg tacggcgcgt atccgtacgg ggtgtttccc tggaggtgtc ctgatcccgt
     6421 tgtcgggttg ggagtgcctc tgtattttcg ggatggacgg gcccacgacc cgtacttcgc
     6481 ggattggggc tgttatccgt acggatgggg gtacgggcag cgtgtccggt tgcggctggt
     6541 cgagccccgt tacgcgcccg cgttatccga tgagttgtcg ctaccgttgc ccggttccgc
     6601 gcccacggaa ctccgcgatc cggaacgcga acgcgcgtgg gaacgggagt ttctacgcat
     6661 tctcggtgac cgggacgagg cggcgtggcg tccggatgcc accaacggtt gcgcccggag
     6721 cccgtgatcc ctgccgtgga gagacggaaa gttggcaggg cgacggatct gccgttgtgg
     6781 gtggggacgc ccgcgttccc gactcccaca cgaaggcatg aacgcgggcg gaacccgcaa
     6841 cctgaaacca aagagcgcgg tgcggtgtct ttcgtatcga ccgcgaaaac caaacatggc
     6901 aaactatggt gtgtggggaa aagcccgccg gacggatgcc gggcggcttc cgccgtgcgg
     6961 atttcctccg gaaaacggaa agctttcttt agaggtgtct gcccttgagc ggatggaaca
     7021 gagtgatggg ttggcttgtg gcggtgttgc cgttgtgtgc cgtgactgcg gccgaatggg
     7081 aaacggcccg tgccgcgctg catgacaagc tgtacccgat cgctcaggcg catgctgaaa
     7141 aggccctagc cgaaggggag gcgccggaaa tcgcgctgct gattctggcc gaagccctcg
     7201 cggcccagga tctgccgcag gaactgctgc ggcggctcga tgcgcatccg gaatggattc
     7261 aacgcgctcc gcagccggag gccttcgcct actggcgcgc ctgggccctg atgcgcctag
     7321 gccagccgct ggaagcggcc cgcatcgcgg agcttgccgc cgccgaaccg tcgtcctatg
     7381 ccgatgcgtt gcggcgtctc gccgcgcgcg cgcgccttgc ggccggggac caggctggtg
     7441 cgctgacgct cttcgccacc gtagaccgca cttcgaccaa ccgcgccacc cgtgtggaga
     7501 atgcgctgga ctgggctctc gcgctcgaac aggccggtcg cacggaggcc gctctggagg
     7561 tgctcaaagc gcaggcggag catgccgaat cgggtacggc catgagcgaa ggcgcgttgc
     7621 tgcgcggtcg gttgctgttc aatctcggcc agacttcgga ggcgacccgg attttcaacc
     7681 aactcgccat gaatgagcgt gccaccggtt cggcgcgggt ccaggcgctg gtggagatga
     7741 gcgtgtacac gctgcaagag ggcaaaacca acgaggcgct ggcctacgcg cgcagcgctt
     7801 acgaccgggc gcggcagccc gagtcccgtc tgctggccgg gttccggttg ggtgatctgt
     7861 tgtccgcttc accggatacc atcgatgagg ccgaaaagat catcaaggcc ctggtgcgcg
     7921 aatttcccga acatccgtgc gccatgcgcg cccacctcaa gttggctgac gcgttgttgc
     7981 aggccaatcg cccggaacgg gcggcggacg aataccgcat cttcctcgaa acgtatccgc
     8041 cctcttcgct gcatgccgaa gtgctgcaag ggcgcggctg ggccctgatg cggctgggtc
     8101 gctatgcgga ggcgggcggc atgttccagc gcgcttccga gacggccacg aacctcgcgg
     8161 aaaaggccga gtgcatcttc aagcagggtg acgcgctcct cgcggacggc cggttcgctg
     8221 aggcttccgc cgcctacgcc aagatggccg gtcagtttcc ccagagcccc ttggccgggc
     8281 gcgcgctcta ccagagcgcc gagtgtctcg aacgcgctgg acggcgcgcc gaggccggtc
     8341 agcagtaccg tcatgtggcg cagaccttct ctgatcgcga agaagcgccc cgcgccctgt
     8401 tgcgttacgc cgcgatgcag gccgaagaca atgcgctgga ggaggccgtg aaaacctaca
     8461 ccgacgtgtt ggaggcgtat ccgcaaccgg cggtacgcgc cgacgcactc atgggtcgcg
     8521 gcaaagccct gtaccgcacc taccggttcg acgcggcgat gcaggatttc gccagtgtgg
     8581 cggaaagcac gcccgcgcgg cgcgatgagg cccgcttcct gttgacgcaa tgcctctacg
     8641 ggctcggccg cgatcgcgag gcccatgccg cgggcaccgc cttcctgctg gcctttcccg
     8701 aatccgattt cctgcccgac atgatgttgt ggctgggcaa gaccaccttc aacagtggcg
     8761 catacgaaga ggcgtcgcgc ttcttccttg attacgtcac ccgctggccc gccggacggt
     8821 gggcggatgc cgcgctgctt tgggccgcgc gcgccgcagc cgctaaaatg gatttcacgg
     8881 gggcggtcga actggtcacg cgtttggcgc gcgcctaccc ccaaagcgcg cgcctgaccg
     8941 aagcactgag ggtgcagggc gacgccctta tggaactggc ccggttcgac gaagcgatcc
     9001 tgctcttcga ccagatcctg ctgaaggcgc ctgcggggga tgaggccaaa caggcgctgc
     9061 tgcgcaaggc ggattgcctg ttcgcgctcg gtgcgggcaa cagcctgagg tatcaggagg
     9121 cgctggaggc gtatcagacc ctgcttgcgg agaacgacct cgcccctgcg ctgacgttgc
     9181 aactccattt caaggccggg cgttgtctgg agaaactgaa acgcgtcgat gaggcgatcg
     9241 accgttatta cgccgatgtc atgctgcgct atcaggacgc gcgctcccgc ggggtttggt
     9301 tcgacgcgac cggcgacagc ctgttcgttc gcgcggcgtt taacgtggcg gatctttatg
     9361 agcagaaggg gcagccggaa caggcggttc cggtgctgcg gcgggtgcgc gattcgggca
     9421 tgcccggaag cgaggaggcg cagcggcgga tcgaacggct gcatcaaacc aagatggggg
     9481 gcatatgacc atcctgatgc aaggcggagc ggtgttttgg ctgatcgtcc tcttgggcgt
     9541 ggctgccgtg atcgtgtttt ttgaacggat gttgaacctg tggcgtgcgc agatcgatta
     9601 cggcgacttc ctgaaagggg tctgtaatgt gcttgataac ggcaaccgcg aagaagcgat
     9661 gatgatctgt gacgagacgc ccggcccggt cgcggcggtg gtcttgaccg cgatccgtca
     9721 cagtcagggc acacccgagg ccatccgcga ggcggtggac aacacggggc gtgccgagat
     9781 ctcgcgtttg gagcggcggc tggcttcgct ggcggtcacc tgccaaatcg ccccgctgct
     9841 aggattgttc gccgcgattc tgggcgggat ccggctgttg ctgacgctga cggagcaggc
     9901 cccggtgatc cagagtctgg atctttcgca gagcttgatg caggcgctga tcgccgccgc
     9961 cgcgggcgtg cttgtggccg cgacctgtca cgtgatgtac gctttgctga tggtgcgtat
    10021 cgaacgcatc gtgctggata tggaggcttc ggcctcggag atcgtggcgt acctcaccca
    10081 tcagaaccgc cctccggtat cataaaaggc tgtgtatggt tagcgagaaa cgagagagtt
    10141 ggagccggca agtccgacgc caccggacgg tctaccgttt cggcggcgct tgggcgcagg
    10201 cgctgctgtc agccataccg tgggtgacga cgcttctgtt ggcgcttttg ctgatgcttc
    10261 tgcacggacg tttggcgatc acgccgggtg tcgcctttga tctgccgacc gcgccgttgc
    10321 gcgaaggatc gcgtgcccgg ttgaccgccc tgatgttgca ggtgacgcgc ggggatctgc
    10381 cctcgggcga ggaaaccctc attttcttcg acgatgaccg ctacgtggcg caggatccgg
    10441 aacaacaact tatgctggcc ggacgcttgc aggaccgcat gaccctgagc gtgcaacgag
    10501 aactggtgct gctggcggac aaacgggtgc cgcacggcga aatcatgcgt ttcgtccaga
    10561 cggcccgcaa ggccggcgcg aagcgcgtgc tggtggcgga gaagccggaa cgcggcttgg
    10621 agcgggagta acggcatgcc ggatggtttc cagagggttc cccgtttccg ggcccgcatc
    10681 acgcaccggc cagtcctgtt ctggtggcag acgtgtctgg tgctggcggt cttttccctg
    10741 ctctggttcg aacttccccc ggaagcggtt ttctatgcgc cgcgcgcgtt tgcgcctctc
    10801 cctgccccgc acgccgcgta tgtctctctg gatcccgagt atgccactga gatgttccgc
    10861 aaaaccgtca tggggtggat gcttggcggt gtgatcggca aagatgaggg cggcatggag
    10921 ttcgggctct cggtgctgag cgacgcgctg ccgccgcccg tcctgttgga acagggcaca
    10981 cggtatccgg gcgtgtggca acccctcgag gtcgacctgc ccgcagtcca gccgtcggtg
    11041 ctgcctgcgc cggggggagc cgcatcctgc gcgccccgcg tgctcgcgcc gccgccggag
    11101 ggcgtgcggg ttgtgccgga tgcagccttg cgcgcggcgg gatgggaggt ggccgtgcca
    11161 gacggggttc tgaccgtgaa cgagggccac ggccgcttct atgtggaaac cgatgcggac
    11221 gggcatgtcg cgcatgtgct ggtttttccg ccgcacacac cctccaccgc cgcgctggag
    11281 cgggtgctcg cgcgtggccg tgcccgcggt gccgcacgcg ggatcgtgga ggttcattgg
    11341 agacgaacgc catgatgtca cgccgcccta tcccgagtcg gcgcccggcc acggcgtacg
    11401 ttgccgtcgc tgcggtcata gcggcggtcg ccgtcatcgg ctggctcgcg cgttcgatgt
    11461 gcgcgccgca gcccgtgcct tctgcggcgc aacccgcggt tcggaacgca tggcgtgtgg
    11521 tttggcgcgc ggcactcacg gcgcacgcca acgcgccggt tccggtggac gcggaaggct
    11581 ggatcgtgac ggacgatgcg ggcggtgtga cccgcctctc cgatacggga aagccattgt
    11641 ggcgtgtctc tttttccaac cagccgttcg aagcctcggc cgcgatacac gatcggcggg
    11701 tgttcgctgc ctccaccagt ggcggggttc tggcgctgga tgccgaaacc ggccgtgcgc
    11761 tctggtctgt gacgctcgac gcgcgtttcc agcatccgcc gttgctcgac gcgcagggcg
    11821 gcggggatgc ggcggtgatg cgcctggtgt cgcagtcgga cgggcggctc ttctgtctgc
    11881 gcgttcgtga cggcctgacc ctctggcaga gtgaaccgac caaccggtgc gacggcgagc
    11941 cagccgccgg accgtacggg atcgcttacg ggaattgcga tggcgcgatc catgtgttcg
    12001 atgccgcgga cggtcggcgt aaaggatcga tcccggtggg cacggaggat cagatggccg
    12061 gcggtctgct ctcgttgccg gatagtcgac tggtggcggg tacgcggcag ggaaatctgg
    12121 tggtggcgaa cgcgcggacc ttcgtgtgcg aggcgcggac gcgggtttcg gagaatgagg
    12181 cgtttctcaa accggtccgg acccaaaacg ggctgatcgc gacgggtagc gaagagggct
    12241 tggtttcctt gtggttcctg cgggatacaa cgctttcgca ggccggggaa taccgctttg
    12301 aacaggcgat ccgtgatctg gccgcttgcg gtaaccggct cttcatcctg accggcggtg
    12361 cgctgcacat cgcggatacg ccggatgctg cggtttgccg tgtggcgctc ggcgacgagg
    12421 tgtacggttt ggccctgcgc gattgcgtga cggcggcatg tgtggcggac ggcatgctcg
    12481 tctgcgtgaa aggggaggct catgagtaac ggcgcggatg tgctggtgac gggtgtggtg
    12541 cggaccttcc gtccgccggg gggggtgccg gtcgaagtgt tgcgcggtgc ggatctgacg
    12601 gttacggcgg gcggaagcgt ggccgtgacc ggcccttccg gttccggcaa gaccacgctg
    12661 ctccatctga tcggtgcgct ggacacgccg gatgcgggtg aaatccgggt gaacggtcag
    12721 cggctcgacg cgcttgagga agcggcgcgg ttgcgatacc gtaaccggga ggtggggttt
    12781 gtgttccaaa atcaccatct gctaccgcaa ctgaccgcgc tggagaacgt gctcgtcccc
    12841 gcatggtccg gaccggatgc gactgcgcgg gagcgcgcgg aacatctgct ggaacgggtc
    12901 ggcctgggcg accggatgcg ccattttcca gggcatctct cgggcggcga acgccagcgg
    12961 gtggcggtgg cgcgggcgct ggtcatggac ccgggactgg tattggcgga cgaaccgacc
    13021 ggtgcgctgg atcagaaaaa cgccgccgcg ctcatcgacc tgctgctgga actgaatgcc
    13081 gaacggcgtg ccacgctgat tgtggtcacg cactcggagt tgtgcgcacg gcgtatggca
    13141 cggcgcgtga aacttgaaaa cggaaccgtg agagaggaaa ccggaccatg acggacaagg
    13201 agattctgaa aacgttggaa gagacgggcg cgctgctgag cgggcacttt gaattgcggt
    13261 cggggttgca cagcaaccgc tttttccaat gcgccaatgt gctgcgttat ccgagggtgg
    13321 ccggtcggct ttgcgaagag ctgacggcgc gcgcccgcgc cgcgaaagcc ctggacgggg
    13381 gggtggacgg cgtgatctca cccgcgctgg gcggcattct ggtgggccat gaggtggcgc
    13441 gcgcgctgga tgtgaagtgc gtgttcgcgg agaaacagga cggcgcgctg gtgatgcggc
    13501 gttttacgct caaaccggga gagcgttatg tggttgcgga agacgtgatc acccgcggcg
    13561 ggcgcgtgca ggaaaccatc gatctggtga aggcggcgga cgcggaagtg gtggccgtgc
    13621 tggtgatggt ggaccgcagc gcgggccagg ccgtgtttcc gtatccgacc ttctcgctgt
    13681 tgcacatggc gcctgaggtt tgggagcccg cggcctgtcc gctctgcgcg cggaacgaac
    13741 cgttcgtgca tccgggttcg tgatggcgga cccgggccgc ggtacgccgc ggcccgggtg
    13801 ccggaatgtc gatcatttgc cgcagcggaa cgcgccagcg ggcagtccga taccgttcac
    13861 cagattcggc tcggcgatct ggttccacgc gaagcgcatg gctttcggag cgctgacgga
    13921 cgacgcggaa aggatcacgc tcgttccgtc gatcttggcg tcagccttga cgaaccggcc
    13981 gtcctccccg catatctcaa accaggtcgg cggctggccg tcgcgggttt tcagcccctc
    14041 ggcgtgatcg aaggtgacgc gcaccgtctg gccctcgatg gcgaaagagc ggtaaaccgg
    14101 accggcccag gcgatttcgc tgcgcccgta agtgcggttg agcgcctgat tggcgagacg
    14161 caggccgaca tcctgcttgt tgcgcggatg gatatcgcgg atgttcccga tatcgttgat
    14221 cacggtgtag ccggtgttgg ggatcgcggc gggcacggcc gcctgggcct cccagatgcc
    14281 gggcagattc tcagggttgc ctccatacgt gtatggcgcg agctgcacga ggtaataggg
    14341 gaaatcgcct tgtccccaca cttggcgcca gcccttgacg agcgctttgg ttttctccag
    14401 ataaagcatg ccttcaccgt tgttcgaaca gccttgatac cagattgccc cgcggatcgc
    14461 atagggcacc agtccggcga ccataccgtt aaacaggact gtgggatcct gattccggaa
    14521 ggagaacacc gaggggtatt ccggcatcgc ggtcaccgcc tgattcgcgg cgagccgctt
    14581 ttcgctctcc gcgatccact cctgcagccg ggtgaaatag gtctggtgcg tggctttgta
    14641 cgacgtcgag ccgggactga cttgctcgac ccgtttctgc gcattgcgga gaatcccctc
    14701 cagcgccggg atcccctcga atccacagag cggggtccag ggctcaatcc gcgtgccgcc
    14761 ccaagaacag ttgatcagcc cgatgggcac gttgagcacc tgatgcaggc ggcgtccgaa
    14821 cagaaaggct accgcggact cgtcgccgat cccggcgggg gaggtctttt cccagacggc
    14881 gttcacgtca tccagcggca gtcccgagac ccgtttgggc gctttgaaat ggcggatcag
    14941 cggcaggttg gccgccgcga tctccttctc cgcatggtct acggagcgca tggtccactc
    15001 catgtttgac tggccggaac agaaccacac ctcgcccacc accacatccg aaaaggtcag
    15061 cgcgttcgcg ccccgcacct ccagccgggc cggctcggac gaggcttgga gcgcatcaag
    15121 tttcagcgac catgtgccgt ctgccttggc cgcggtctcc ttgctctgtc ccgcaaaggt
    15181 gacggttacc cgctcacccg gcgcggccca tccccaaacc ggcaccgcaa cctgttgctg
    15241 caagaccatg ccgtccccga aaattttcgg catccgaacc gcagcctgaa ccgaaccggc
    15301 aagcagtatc gcgcctaccc accccaactt ccgcattttc ttcattccgt ctcctttttt
    15361 tcgcgtgttg tttgcgggac acccccgcgt cccatgaaat cccttcggca tgtttggcga
    15421 acagccttaa tttttctttt ccggtccgat ccgctccagc gagatgcgct tgaaccaggg
    15481 tgccagacgc ttgaactccc cgctgtcctg aatccggaat ccgttcgtat ccgggtcgac
    15541 actgtccggc agcggggtct ccaggaacgt cacccggcgc ttgccctcat cggtctgggg
    15601 gaaagccatc acatcgcgct tgtcgatcgg cccgaacgag aggttgttct cgaacgtcag
    15661 gcggtgcacg acgccggtgc gcaccacgtt gttcgcgttc aggaagagtt tgcagttgac
    15721 cgccacgttc tcgcggaaga cgttgtgcag cggcagcttg ggctcatcct ccatgatgtt
    15781 caccagatgc ggatatttat ccttccaagg ggactcctgc cacttgaccg cctgaagttt
    15841 ggcgagcagg tcccacccgt ctttcgtgcc ctcgcccgga cgcgcgcgct tgaggccgcg
    15901 attatccagg tgcaacgcgg aggtgcactc gataaacaga ttgttccgga ccgtgttata
    15961 ccgtccgccg cccacgaagg cggcccagcc cgcgcggtag aagatgttgt cgcagatcgt
    16021 atcgccgctg tcgcagtcgt ccaaatagac gcccatgacc atcacggact ccttgagcgg
    16081 ctcgtaatcg ggattcagcc cccgcgcctt ctgccaatcc acgccaggct ggccgaagtg
    16141 atgcagatag ttgtggcgga tcacattccc ctgcgagcac cagttgcgcc cggtatagag
    16201 cccgccgccg tctccgctct ccatcatggc cgaatgcacc tcgttgtatt cgaagagatt
    16261 ctcgttgccg ccataggtca ccgcgatgta cggcgcgtca tagatcaggt tttgcgcgat
    16321 acggatcccc acgccgttga agttgaggca cttgccctgc gtacgctgta gccgcccgct
    16381 gtgatgcagg cggcagttgg tgacgaaatg gtcacaggcg gtcagcgatt tgcggtcgcc
    16441 acccgaaacc gaaacgcagg tgcctcccac gccgtacacc tcgcaccggt tcaggccgca
    16501 ggcccgtccg cccgtgatgt ggatcccgtc cagggtgatg tccgagacgc gcagattttc
    16561 aagccgcacc tcttcgcatc cgttcacgga aacagccaga ccggtgttgt aacggaaggt
    16621 cagtccgtcc agtcgaacat accgcacttt gttcagacgg aacaggggct tcttttgatc
    16681 ggttagcacg acatcgccaa ggccgctgtc ggttggatag aggtagagga ggcacgcctc
    16741 acggtccaca taccactcgc cgggcatatc gagttcttcc agcaggttga aaacatagta
    16801 acgccgtttg gccgtatgcc acttggagga gttgccgatg ccgtaagtat ggatcgcggc
    16861 cgaggtgatc tgttttttct ctttgtcgat cgcgccgatg cggagggtct cgttggccca
    16921 gtcgtggcac cagaacccgt tcatccagat tcctttggag acatcccagc gtgcgggcgc
    16981 gtcctcggcg tactcgaata tgccgccgcg cacaccgaat tcccattcac cggtggcccg
    17041 gtctattggc ttcactcccc ggtcgatcac cttggcgatt tgtgcccagc ccgtgttggg
    17101 ccagcgcgcg agccgcatcg ccttgccatt gctgaaaagc tccatttcgc gccagccgtg
    17161 gaacttgtcc ggcaagggcg cgagccgggt gacgccgatg gtcttgaggt cgaacgccac
    17221 caccttgccg cgcaccgcct ccggcaggcg cgccagcgtg gccgcatcgg aaaccggctt
    17281 gaagccttcg gccggcaatc gccgtccgcc caggaaaagt gcgcccgctt tcgcggcccg
    17341 cacgattacg ggcgcgttcg gcccgatccc gctgtccgat tcttcgaaag acaccgtctc
    17401 ctcgggcatg acgaacgtgc cgctgacctc gaccgtcacg cccttctccg gaaacgtctg
    17461 attcgttttg agctcgcgga tcgccgtctg ggcccgtttc aggctcgcaa acggacgggc
    17521 ggcgctacca tcctgcgtgt cctgcccgcg ctgcgcgtcc acatggaaaa ccgtttgcgc
    17581 gcttgccccc gcaaccgcca ttgccgccat cagtccgacc atccatccga atcttcccgg
    17641 ctcacgcata tccgctcccc ctttaacgaa tgttaataca acagaatgcc gaactataac
    17701 aaaacgccgg ggacgcagac aggacaaaaa gcgtcttgtc gaggtcaaaa caaaactgga
    17761 cggtttgtag ctgtcaaagc gggactcggt cctgtccggg gggacggggt cgcggggctc
    17821 ttccccaacg aggcctcgct gctcaggctc gtgtcagccg tgctgtcgga gaccagcgag
    17881 gaatgggaga ccgggagaat ctatctcaac atggagggca tgccggcatg acccgcctga
    17941 cgaatttaca gacaaaaccc ttgcgctgcc gccggaaggt gtacgccttg cccttgacca
    18001 gcgcgtactc tgcggagccg ggcgtgccga agccgccgtc gcagtggcgg acga
//
LOCUS       DAOSLE010000048        18020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3337_length_18020_cov_7.588366, whole genome shotgun sequence.
ACCESSION   DAOSLE010000048 DAOSLE010000000
VERSION     DAOSLE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 18020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18020
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3337_length_18020_cov_7.588366"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(453..1964)
                     /gene="cls"
                     /locus_tag="PK576_04955"
     CDS             complement(453..1964)
                     /gene="cls"
                     /locus_tag="PK576_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOE60974.1"
                     /translation="MMICLAAQSIDLNTSLGYGTILHYTLSLLVFFHLMLKPRDARTS
                     LLWIFFTTAFPFFGPLAYVLFGINTVPNKGWQKQYSDSLFEKRQRLNLRQSHPLASMA
                     AQRNTLQITSPDLEALPFNRVLDHLSNNHPLLGGNTIELLEPAGDALEEMFSAIQQAT
                     DHIHLATYILNDDAVGRRLMALLAERAAAGIKVRVLYDAFGSAGASLRLFFWRYRRVP
                     NLHLIGFSQANVFKRKFQLNLRNHRKILVIDGTVAFTGGINFHDVYLPRHDKPGTLDY
                     HFRIRGPAVLELQYTFLRDWYYMTDIPPEKLLAARFFPTPEPTGNCAVRLQNSGPTRD
                     ETAAALDTYFAAINLARSQILLVTPYFVPPEALIRALRQAAFRGVEVKVLVPSVNNHP
                     TVQLASQALYTTLIKAGVRIFERSPPFLHAKAFLADDTIAIIGSANLDSRSLYLNYET
                     NLVVFDTEFAARLKRVILHDFAKSREILYSNWRHRSRLRRLGENFFNLFHPIA"
     gene            complement(1961..2401)
                     /locus_tag="PK576_04960"
     CDS             complement(1961..2401)
                     /locus_tag="PK576_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60975.1"
                     /translation="MRHHRVEQWEARLNALLKRVDHALEERFGDLLTPHPARPPHGTT
                     ANPQYDGLFRVTAAFTAGFGSSLGKGYVLSIEPVSLEPLPKKARAKIEATAVQMIQEG
                     LEEALPGRGLRVERDGTVWKIVGDLSLDNPQQRAAAHPPQKENT"
     gene            2507..5575
                     /locus_tag="PK576_04965"
     CDS             2507..5575
                     /locus_tag="PK576_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOE60976.1"
                     /translation="MRDYGLRVVVLVVAGLVAGAGDAAGLLKNGGFETGGTTPDGWRL
                     QDAQGGWLRQGGYAEGACVRIVNETVGNGAWVSDPVGFKPGHCYRLTLRARGEDASGG
                     TALYGTPFAHVDIGVPSGTWERYGHVFAVPFGKEWRDVPVRLGMWQMQGQVLFDDARL
                     VPVEPVYALSEGLALGEGERLEAGRYHFVAPFRSEGRNHSRPLLGFSARFNTDRWCFD
                     AGTVVTYRHTVTGYVMRTASVSVNSGYYASGRLVVEFSADGKTWQQGGALTNAGSLTV
                     AVPEAMLPAAALSVRLRGAKPPCAVQVHGYRFDAEVEGPPRSFIGSTRYVETVSREPN
                     PRVRVIGLGDALPGGENVAVLQVRHSGAGPLTNEARVVFAGKGQPVRPHPVPIILPGP
                     GAYDVRIPYEAPSAGEWEMVVGLGEAFEARCAVTVPMLYDDSYGERLPVNVPLLSLWR
                     AASGWKIPRHRPLPRAFAKSLALRLARNEGEAVQLVVTPHVTLTNLTVTVTDLVYGKN
                     RIPASRVRVSRVGYVPVEQTSDAAGVRGLWPDPLLPQQGSLIAAAGQHHPFWIRVHAP
                     DDIPAGIYRGTVTIQADGVKATTVLNAEVYDFTLPDTMTCETAFGFSASTVWRYHKAT
                     GPEQRRAVLDLYLRCLGEYRLSPYDPAPMDRWSVTWRGFDTRTGKPEPGKKIAEIEPV
                     FHWEEWDAAMTRAFESNHFNTFRIKVEGLGCGTFHARTEPVFLGFAGGTPEYELLMGK
                     YLRGIEAHLREKGWLDKAFVYWFDEPDPKDYDFVMNGMNTLKRHAPGLRRMLTEQIEP
                     KLFGGPHIWCPLTPSLKKTGLAERREAGDHFWWYICCVPKAPYVTEFIDHAGPALRVW
                     LWQTWAERVAGVLIWETTYWTSDTAYPESGKPQNPYTDPMSWTTGYGVPKGTKRPWGN
                     GDGRLLYPPLAAADGNPAGPVMDAPVPSLRLEMLCDGIEDYEYFVILQRLLQQKGGSL
                     DPRTRQNLEALLTVPAEVSAGLTDFTRDPAPIETHRDKLARAIVFLLRLR"
     gene            complement(5638..6405)
                     /gene="kduD"
                     /locus_tag="PK576_04970"
     CDS             complement(5638..6405)
                     /gene="kduD"
                     /locus_tag="PK576_04970"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HOE60977.1"
                     /translation="MILDQFKLDGKVALVTGSARGIGQACAIALAQAGADIALVYNRT
                     PADETAKAIRSTGRRAESFQADLSAGVDVAQELVTRVAEAFDGVDILVNNAGIIRRAP
                     FAEHSVKDWEDVLAVNLSAPFYLSQAVARRMIAQGRGGKIIHIASMLSFQGGVLVPGY
                     AASKGGIRSLTMLMANELAPHNICTNAIAPGYIATENTRPLRENPARNQAILSRIPAG
                     RWGTPEDLMAAVVFLAAPASDYMNGHTLAVDGGWLAR"
     gene            6593..8965
                     /locus_tag="PK576_04975"
     CDS             6593..8965
                     /locus_tag="PK576_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60978.1"
                     /translation="MRRWVSLRLTQAAGYPSLTRRCCAWVTLFTALSATVPTARGEED
                     RNFEALPLQVQYRVLLEPWIRAAMTEIRFLQDDMAVYGLGQSGHWHMQAHDTAFCAFA
                     VLGTDPQTDEARVGMTKAELRRIALAMLRYTLRSHHSGGGVCTDGKSWGHTWISSLGL
                     ERMSVGIDALEACLDETLRALYAKVCASEADYLLTRPVTAGLIRNNHPESNQWQGNVT
                     HRAAVLNPGHPHAADWREQASVLLVNSLSTPSDETRSALVDGKPVSARFVGANMFDSR
                     ACNHHGYLNVGYMYITQSNLGIYHFACKRRGVRPPEALYHNALEQWRVNKACTFDDGR
                     LARLGGDSRVRYAYGQDYAIPVWLLARDVFGDADAERFERGWLRQVRAEQKANSDGWF
                     MRERLSGLQALSPLYFQRLEGDKACTLAYGAYWRRVFDFDAMPRRSVDAPTRWQDAHH
                     GAWLQRGTRRFASWVWHGAMKACGLCVPSDRSDMAEWQHNLAGQVCGMGAQNVAVPVT
                     WTGYAYEGGFATCGRAEMISKNGIAEGSEVPGLGQIDLAFCALPDDRTTVVIQRAVTR
                     NPCYLLRVRGLSLSVPNDALNGFTRTLQSAAGRRVLRALPDRFEIVSPEGRWLNLDGR
                     LGVVELYGGAPHVARLPERQIPLNIWNSAGPKRGGGYLYCEEIGQNIREGAAYFGARA
                     TIFDCAAAVVVADAADTAAIAADRETGAVPLADADLRAVRVRDARGTLWGLLANFSTE
                     AKSLDSEPVSGWLGSGAVPLHAATHQIAPHGIAVWRLAGQGDVTTAGVRP"
     gene            complement(9066..10202)
                     /locus_tag="PK576_04980"
     CDS             complement(9066..10202)
                     /locus_tag="PK576_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010420354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOE60979.1"
                     /translation="MGWVTACVTPKFSGLDMSLGTLPRLSEAETRSISPENFTGAKGA
                     GAQSTNGPAWRAARDLGQGWKVSPFVNVPAKSTVTLADIAGSGAIQQIWMTPAPLDKT
                     RLNILRFYWDGETSPSVEVPLGDFFACGWGKYCQINSTPVCVNPGSAFNCYWVMPFRK
                     GAKVTLENLDENPMRLYYQINYTLTDVPADAATFHAQFRRVPRVGERPEVTILDGVRG
                     HGHYVGTYLAWEVRDPGWWGEGEIKFFLDGDETFPTICGTGTEDYFCGSYNFENRETK
                     RYQTFSTPHSGLAQVLPPDVIYRTGQRFGLYRWHVVDPIRFAKDLKVTIQTLGWMRDK
                     SGRYLPLHDDVASVAYWYQTEPHAPFPALPPPEVLKVGPLQVAP"
     gene            complement(10247..11698)
                     /locus_tag="PK576_04985"
     CDS             complement(10247..11698)
                     /locus_tag="PK576_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE60980.1"
                     /translation="MTSSPTPAYAPVTPAIVDAVRAIVGPKQVVTDEEKLQAYSHDET
                     NAEEYGRMPELVVFPTSTEQVAAVVRLANRERIPITPRGAGSGLSGGAIPQFGGIVIS
                     LEKMNRLIELDTANMTVTVEAGMVTNDLAALVQEKGLFFAGYPMSLQTCMIGGNIAEN
                     AGGGKAVKYGVTGRYVTGLELVTPTGECVWLGGKLAKDVTGYDLKQLVIGSEGTLGIV
                     TQATIRLIGYPTAKSDLLVLFRTPREAIDMVPIIMSKGLVPTSIEFMDRLSILTSCRY
                     LNESLPYEAAGAMLLIETDGSHPGQVEQDLIAIGDLCEEHGAIEVFVAEDRNTIERIW
                     NVRRNIAEAFKVYSPIQSLEDIVVPPSRIPEIIPELEKLSAKYGMRIPCYGHAGDGNL
                     HATLVKDPDMPMEAWHTAEKACLNDLYRITRRLGGKISGEHGIGIKRKAYLSDLIDPV
                     ELNLMRAIKRAWDPNHIMNPGKIFDLTSQIDES"
     gene            complement(11700..12701)
                     /locus_tag="PK576_04990"
     CDS             complement(11700..12701)
                     /locus_tag="PK576_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOE60981.1"
                     /translation="MSGVWTLAEQAHGELKGVSFELLARGRKLADQLGVPLVSVLIGD
                     GVEDSRLHELIARGADEVYAVQDPRLAHFVCETYARILTHLINERKPDIILAAATSSG
                     RTLMPYVAVRVRAGLTADCTELDIEEGTGNLLQTRPAIGGNIMATIKTPHHRPQMATV
                     RPKSTRPLPPDPTRRGTIMSIPLDASLVDPRVTVLGYRRDVSDFVNLEESDIVVAGGK
                     GLKKGEHFAMLRRLAETLGGVVGASREAVDRGWIGYPHQIGLSGKTISPKLYLGAGIS
                     GSIQHLAGIKTSEIIVSVNPDPDAPLHQVADYAIVGDAFQILPALQRRLEARKGKKA"
     gene            complement(12698..13510)
                     /locus_tag="PK576_04995"
     CDS             complement(12698..13510)
                     /locus_tag="PK576_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE60982.1"
                     /translation="MKIIVPIKQVPETSNVKMDPETGTVIRAGIETVVNPLDLYALEA
                     ALRLKEQYGGTVTAITMGPPPAVKALKEAVAMGCDNAALVSDRAFGGADTWATSYTLA
                     QAILHMGAFDLILTGERATDGDTAQVGPGIAAWLDLPVATYVARIEGIAKGRLTLERL
                     VEEGYQQLSVPLPCLLTVVKEIAAPRLPTLRGKKAAMRMEIPVFSTANLPLDSGCIGL
                     KGSPTKVVRINTPKVTRGGRKVLATDDGAVEAAVDGLIAFLDEKGLIEGERQ"
     gene            13643..14638
                     /locus_tag="PK576_05000"
     CDS             13643..14638
                     /locus_tag="PK576_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOE60983.1"
                     /translation="MIIRTTAYPRAALIGNPSDGYYGKTIAFTFSNFSARVILYESPE
                     LEIMPTRRDGSVFTSIGDLFQDVRLFGYYGGIRLLKAAIKSFYEYCRENEIELHDRNF
                     TVRYDTDIPNLVGLAGSSAIITATMRALMAFYGVTIAKPRLANLIRAVENRELGIAAG
                     LQDRVAQVYQGLVYMDFDQALMERQGFGRYEPLDVATLPPLYIAFRTDLSEGSEVVHN
                     NLHDRFERGEPEVLAAMTYWADLTAQARQALEKGEGGELGRLMNANFDKRREICAIGE
                     GNLRLVETARQVGASAKFTGSGGAIIGTYPDERTYTRLTEVFAAQQVCVFKPDIL"
     gene            14655..15548
                     /gene="galU"
                     /locus_tag="PK576_05005"
     CDS             14655..15548
                     /gene="galU"
                     /locus_tag="PK576_05005"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018620729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOE60984.1"
                     /translation="MITKAVIPAAGFGTRFLPATKALPKEMLPILDTPVIQYVVEEAV
                     ASGITDIVMVIGRGKRAVEEHFDRSFELEAELEAKGRLRELETIRAISELANIHFVWQ
                     KRMLGLGDAIRCARYHVGDQPFAVLLGDTLLQSLSGVPATRQLMEAQSRHGGCVVAVE
                     EVPRERVSQYGIISGEPLDAETIRIRDLVEKPAPDEAPSQWAVASRYILTPDIFARID
                     AATPGRNGEIQITDALRARALEGEPLFGFRVAARRHDIGNMLGFLTANIRYALGHAEL
                     AGPLGAFILAEAEAIRRARGE"
     gene            complement(15620..15817)
                     /locus_tag="PK576_05010"
     CDS             complement(15620..15817)
                     /locus_tag="PK576_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60985.1"
                     /translation="MKQLLMIAVMAAATCAFVGCKKEESLGDKMDKAVKSAEKSANTA
                     AKDTEKAAGDLQKKLDGALKK"
     gene            complement(16463..16825)
                     /locus_tag="PK576_05015"
     CDS             complement(16463..16825)
                     /locus_tag="PK576_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOE60986.1"
                     /translation="MDIYVGNLAYTTSDESLKVAFEAYGEVTSARVVSDRMTGRSKGF
                     GFVEMPDRTQAQAAIDALNGKELDGRTIRVNESQPKPREERGGGFRGGAGGGGGFRGG
                     SGRGGAGGRGGERRESRW"
     gene            complement(17248..>18020)
                     /locus_tag="PK576_05020"
     CDS             complement(17248..>18020)
                     /locus_tag="PK576_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60987.1"
                     /translation="WIMAFAAGHDAGVDFQFGLPKTYEKCERIRLWEDARRASAFTET
                     EKRLMRQTDTLWELKREDGKFTLSFVRRWKGERFEELPASAAPVKALGTGTAVMPRSI
                     NRKWVHASGQCERWCITDDLIAKVSVKPADFEVVSPIDNRKWAMTVPLFIMRAPENAS
                     PVVNPVTHYRWGNRRGKIRVKTTLAPGEYVTIPHRVSLAVVYNARHELLREVALEDIP
                     RASFAKGDTVGVSFTSDNTEPVPLCVNLRFIESSIEEK"
BASE COUNT         3325 a         5245 c         5907 g         3543 t
ORIGIN      
        1 gggtcgcggg tcgacgatcc cgattccgat agcgattccg atcccgaccc cgatttggat
       61 gaaaccctga aaccctgaaa ccctgaaacg gaaccttcat gaaaagcagg gttggtgttc
      121 aaagcgcaat gctgtttgtg gccggattcg taacgcgaca aggtctgtat ggttcaggtg
      181 ccagcggacg gcgccatgtc cttttgcggc tgaacgtcgg cgtgaaccgt agacgatccc
      241 tgccagtacg gcgggacgtc cacagctgcg cgccgtaggc gcggagccgg accgctgaaa
      301 ccgtgcgggc tccgggtaac gatgtttcag gccgggaagg acaagcatct gcttgcccgg
      361 tcgagcaggc acccgtcccg agcattttca tttcatgagg tttcatgtgt ttcatggtac
      421 ggaacctaaa atccagaaac caatttacct cgtcaagcga tgggatggaa aagattgaaa
      481 aagttttcgc ccaaccggcg gagccgcgaa cggtgccgcc agttcgaata gaggatctcg
      541 cgggatttgg cgaaatcgtg caggatgacg cgcttgagac gggcggcgaa ttccgtgtcg
      601 aagacgacca ggttggtctc gtagttgagg taaagactgc gcgagtcgag attcgcgctg
      661 ccgataatcg cgatcgtgtc gtcagccaga aacgctttgg cgtggaggaa ggggggggaa
      721 cgctcaaaga tgcgtacgcc tgctttgatg agagtggtat acagggcttg cgaggcaagt
      781 tgcacggtgg ggtggttgtt gaccgacggc accagtactt tgacctccac gccgcggaaa
      841 gccgcctgac gcaatgcgcg aatcagcgct tcgggcggca cgaaataggg ggtgaccagc
      901 aggatctggc ttcgggcgag attgattgcc gcgaagtacg tatcgagcgc ggcagcggtt
      961 tcgtcgcggg tggggccgct gttttgcagg cgcacggcgc aattccccgt gggttccggc
     1021 gtggggaaaa agcgggccgc gagaagcttt tccggcggga tgtcggtcat gtaataccag
     1081 tcgcgcaaaa atgtgtactg gagttccagc acggccgggc cgcggatgcg gaagtgataa
     1141 tccagcgtgc ccggtttgtc gtgccggggc agatacacat catgaaagtt aatgccgccc
     1201 gtgaaggcca ccgtgccgtc gatcaccaga attttccggt ggttgcgcag gttgagttgg
     1261 aacttgcgtt tgaacacgtt cgcttgggaa aacccgatca gatgcaggtt gggcacccgc
     1321 cggtaacgcc agaagaagag gcgcaggctc gcgcctgccg aaccgaacgc gtcatacaaa
     1381 acccggacct tgatgccggc ggcggcgcgc tccgcgagca aagccatgag gcgccgtccc
     1441 acggcgtcgt cattcagaat gtaggtggcg agatgaatgt ggtcggtcgc ctgctggatc
     1501 gcggaaaaca tctcttccag ggcgtccccc gctggttcga gtaattcgat cgtgtttccc
     1561 cccaacaggg ggtgattgtt gctcaggtgg tcgagaacgc ggttgaacgg gagtgcttcg
     1621 agatcgggcg atgtgatctg caaggtgttc ctctgggcgg ccatcgaggc cagcggatgg
     1681 gattggcgca ggttgaggcg ctggcgtttt tcgaagaggc tgtcggaata ctgtttctgc
     1741 caccctttgt tgggcacggt gttgatgccg aagagcacgt acgcgagcgg tccgaaaaag
     1801 ggaaacgcgg tggtgaaaaa aatccagagc agggaggtgc gcgcatcccg cggcttgagc
     1861 atcaggtgga aaaaaaccag cagcgacagc gtataatgca gaatcgtgcc gtatccgagc
     1921 gacgtgttga ggtctatgct ttgcgcggcc aggcaaatca tcatgtgttc tccttctgcg
     1981 ggggatgcgc ggcggcgcgc tgctgggggt tgtcaagcga gaggtctccc acgatcttcc
     2041 aaacggtccc gtcgcgttcg acccgcaagc cgcgtcccgg caacgcctcc tccaaaccct
     2101 cctgaatcat ttgcaccgcg gtcgcctcga ttttggcgcg cgccttcttt ggcaggggtt
     2161 ccagcgagac cggttctatg gagaggacat agcccttgcc taaactggaa ccgaaccctg
     2221 cggtgaatgc ggcggtgaca cggaagagtc catcatactg cggattggcc gtggtgccgt
     2281 gcggcgggcg tgcggggtgg ggcgtcagca ggtcaccgaa ccgctcttcc agcgcatggt
     2341 ccacccgctt cagcaatgcg ttcaaccgtg cttcccactg ctcgactcgg tgatgtctca
     2401 tccgattatc atatcataat ccgtatgtcg ctttaaatgt gattcttgtt tccggaggca
     2461 tttctgacgt atactgcgga aaggaatgca acgttgggag gggacgatga gggattacgg
     2521 tttgcgagtc gtggtgttgg tcgtggccgg tctggttgcg ggggcagggg atgcggccgg
     2581 gctgctgaaa aacggggggt tcgagacggg tggaacgacg cccgacggct ggcgtctgca
     2641 ggatgcgcaa ggcgggtggt tgcggcaggg cggatacgcc gagggtgcct gtgtccggat
     2701 cgtaaacgaa accgtcggca acggggcgtg ggtgtccgat ccggtcggat tcaaacccgg
     2761 ccactgttac cggttgacgt tacgtgcgcg gggggaagat gcgtccggcg gaacggcgct
     2821 ctacggtacg cccttcgcgc atgtggatat cggcgttcca agcggcacat gggagcgcta
     2881 cggccatgtc ttcgccgtgc cgttcgggaa agagtggcgc gacgtgccag tgcgtctggg
     2941 aatgtggcag atgcaggggc aggtgctgtt cgatgacgcg cgtctggttc cggtcgagcc
     3001 ggtttatgct ttgtcggagg ggcttgcgct gggtgagggc gaacggcttg aggcggggcg
     3061 ttaccatttt gtcgcgccgt ttcggagcga gggacggaac cacagtcgcc cgctgttagg
     3121 tttttccgca cgtttcaaca ccgaccggtg gtgcttcgat gcgggaacgg tcgtgacgta
     3181 ccggcacacg gtgacggggt acgtgatgcg gacggcgagc gtgtcggtca acagcggata
     3241 ttatgcgtca gggcggctcg tggtcgagtt ttcggctgac ggcaagacgt ggcagcaggg
     3301 gggcgcgctc acgaatgcgg ggagcctgac ggtggctgtg ccggaagcca tgttgcctgc
     3361 ggcggcgctt tcggtccggt tgcgcggggc gaagcccccg tgtgcggtgc aggtccacgg
     3421 ctatcgtttt gatgcggagg tggaggggcc gccgcgttcg ttcatcggtt ccacgcgata
     3481 tgtggagact gtatcgcgtg agccgaaccc gcgcgtgcgg gtgatcgggt tgggtgacgc
     3541 gctgccgggc ggcgagaacg tcgcggtgtt gcaggtgcgt cattcgggtg cgggtcccct
     3601 gacgaacgaa gcgcgggtcg tgttcgcggg taagggccaa cccgtgcgcc cccatcccgt
     3661 gcccataata ttgccggggc ccggggcata tgacgtgcgg atcccctatg aggcgccttc
     3721 ggccggcgag tgggagatgg ttgtcggatt gggggaggcg tttgaggcgc gttgcgccgt
     3781 gacggtgccc atgctctatg acgactccta tggggaacgg ttgccggtca acgttccgtt
     3841 gctcagccta tggcgtgccg catccgggtg gaagatcccc cgccaccggc cgttgccgcg
     3901 cgccttcgcc aaatcgctcg cgttgcgtct ggcgcgtaac gaaggcgagg cggtgcaact
     3961 ggtggtcacg ccccatgtga cgctcacgaa tctgacggtc acggtgacgg atctggttta
     4021 cgggaagaac cgcattccgg cttcgcgggt gcgggtgtcg cgcgtgggat atgtgccggt
     4081 tgaacagacg agcgacgcgg ccggggtgag ggggttatgg cctgatccgc tcctgccgca
     4141 acagggatcg ctgattgccg cggcgggaca acatcacccg ttctggatcc gggttcacgc
     4201 gcccgatgac atcccggcgg gcatctaccg cgggacggtg acgatccaag ccgacggggt
     4261 caaggccact acggttttga atgccgaggt gtatgatttc acgctgccgg acacgatgac
     4321 ttgcgaaacc gctttcgggt tcagcgcgag cacggtctgg cggtatcaca aggcaaccgg
     4381 tcccgagcag cgtcgcgcgg tcctcgattt gtatctgcgt tgcctgggcg aataccgcct
     4441 ctccccctac gatccggccc cgatggaccg gtggagtgtg acatggcggg ggttcgacac
     4501 ccggacagga aaaccggagc cgggcaagaa gatcgcggag atcgaaccgg tcttccactg
     4561 ggaggaatgg gatgcggcca tgacccgtgc gtttgaatcc aaccacttca acaccttccg
     4621 gatcaaggtc gaggggttgg ggtgcgggac cttccatgcg cgcacggaac ctgtgtttct
     4681 cggcttcgct ggggggacgc ccgaatatga gttgctgatg ggtaaatatc tgcggggcat
     4741 cgaggcgcat ctccgggaaa aaggatggtt ggacaaagcc tttgtttact ggttcgacga
     4801 gccggatccc aaggattacg actttgtgat gaacggcatg aacacgctca agcgccacgc
     4861 acccggactg cgccggatgc tgacggagca gatcgagccg aaactgttcg gcggaccgca
     4921 catctggtgc ccgctcacac cgtcgctcaa gaaaacgggt ctggcggagc ggcgcgaggc
     4981 gggcgaccac ttctggtggt acatctgttg cgtacccaaa gcgccgtatg tgacggaatt
     5041 cattgatcat gcggggccgg cattgcgcgt ttggctctgg cagacctggg ctgagcgggt
     5101 tgcgggtgtc ctgatctggg agacgaccta ttggaccagc gacaccgcgt acccggagtc
     5161 cggcaaaccg cagaacccct acacggatcc gatgagctgg accaccggat acggtgtgcc
     5221 caaggggacc aagcggccct ggggcaatgg ggacggccgg cttctctatc cgccgcttgc
     5281 ggcggcggac ggcaatcccg ccggaccggt gatggacgct ccggtgccga gtctgcgtct
     5341 ggagatgttg tgcgacggaa tcgaggacta cgaatatttc gtgatcctgc agcggctgtt
     5401 gcaacagaag ggcgggagtc tggatccccg aacccgccag aatctggaag cgttgctgac
     5461 cgtgcccgcc gaggtgtcgg caggcctgac cgactttaca cgcgatcccg cgccgattga
     5521 aacgcaccgt gacaaactcg cccgcgcaat cgtcttcctg ctcaggctcc ggtaagccta
     5581 aaccgaaccg ttgaacgttc aacgctcgtt ggtttcaggg tcctcgatcc ggagcggcta
     5641 tcgggccagc cagccgccgt ccaccgcgag tgtgtgaccg ttcatatagt ctgaggcggg
     5701 ggccgccaga aaaaccaccg ccgccatgag gtcttcgggc gtgccccaac ggccagccgg
     5761 aatccgcgac aggatcgcct gattgcgggc cggattctcg cggagggggc gcgtgttctc
     5821 cgtcgcgata taaccgggcg cgatggcgtt ggtgcagatg ttgtgggggg cgagttcatt
     5881 ggccatcagc atggtgaggc tgcggatgcc gcctttggag gcggcatacc ccggcaccag
     5941 gacgcctccc tgaaacgaga gcatcgaggc gatgtggatg atcttgcctc cccggccctg
     6001 cgcgatcatg cggcgcgcga cggcctggct gaggtagaac ggagcactca ggttcaccgc
     6061 aagcacatct tcccagtctt tcacgctatg ctcggcaaag ggggcgcgcc ggataatgcc
     6121 cgcgttgttc acgaggatgt ccacaccatc aaacgcctcc gcaacgcggg ttaccagttc
     6181 ttgcgccacg tccacgccgg cactcaggtc ggcctgaaac gactcggccc gtcttccggt
     6241 tgaacggatc gcttttgcgg tctcgtccgc aggtgtgcgg ttgtagacca gtgcgatatc
     6301 cgcgccggct tgggcgagcg cgatggcgca ggcctgtcca attccgcggg cgcttccggt
     6361 gacgagagcg actttgccgt caagtttgaa ttggtcgagt atcatttcgg aacccctttc
     6421 gattcagtac gggtggagaa aattacggaa acagtgtgag tgtacccgct tttcacgcgg
     6481 tttcaagtcg aaaccggttg ccaaaacggc gtcaaaccgc aacactatgc tttggactgc
     6541 aggtgaagga gaacacaggg caaggcccgc atgcagtctg agggggggga gcatgaggag
     6601 atgggtttcg ctgcggttga cacaagcggc cgggtatcca agcctgacca gacgctgttg
     6661 cgcgtgggtc actctgttca ctgcgctgag tgcaaccgtg ccgacggcac ggggagaaga
     6721 ggaccggaac ttcgaggcgt tgccgttgca ggtgcagtac cgcgttctgc ttgagccgtg
     6781 gatccgtgcg gccatgacgg agatccgttt tctgcaggac gatatggccg tgtacggctt
     6841 gggacagtcc ggacactggc acatgcaggc gcacgatacc gcgttctgcg catttgccgt
     6901 gctcggaacc gatccgcaaa ccgatgaggc ccgggtcgga atgacgaaag ccgagttgcg
     6961 gcggatcgcg ttggccatgt tgcgttacac gctccgctcg catcattccg gaggcggcgt
     7021 gtgtacggac ggcaagtcgt ggggacacac ttggatatcc agcttgggac tcgaacggat
     7081 gagcgtggga atcgacgcgc tggaggcgtg tctcgacgag acgttgcgcg cgttgtacgc
     7141 caaagtctgt gcctctgaag cggactatct gttgacgcgt ccggtcacgg cgggcctgat
     7201 ccggaacaat caccccgaat cgaatcagtg gcagggtaat gtcacacacc gggcggcggt
     7261 gttgaatccc ggtcatcccc atgcggccga ttggcgcgaa caggccagcg tgctgctggt
     7321 caactcgctt tccactccct cggatgaaac ccggtcggcg cttgtggacg gcaaacccgt
     7381 ctctgcgcgg tttgtcgggg ccaacatgtt cgactcgcgc gcctgcaacc accacgggta
     7441 tctcaatgtg ggctacatgt acatcacgca gtccaatctc gggatctacc attttgcctg
     7501 caaacggcgc ggcgtgcgtc cgcccgaggc gctctatcac aatgctctgg agcagtggcg
     7561 cgtgaacaag gcgtgtacct ttgatgacgg gaggttggcg cggttgggcg gcgacagccg
     7621 ggtgcgttat gcctatggac aggactatgc gatcccggtc tggctgctgg cgcgggacgt
     7681 gtttggtgac gcggatgcgg aacggtttga gcgcggatgg ctcaggcagg tccgggcgga
     7741 acagaaggcc aattcggacg gctggttcat gcgggagcgt ctgagtgggt tgcaggcact
     7801 ctcgccgctc tatttccaac gcttggaggg ggacaaggcc tgtactctgg cgtacggtgc
     7861 gtactggcgg cgggtcttcg atttcgacgc catgccgcgt cggtcggtcg acgcgccgac
     7921 ccgctggcag gacgcgcacc acggcgcgtg gctgcaacgg gggacgcgcc ggtttgcctc
     7981 gtgggtgtgg cacggcgcga tgaaagcttg cggtctctgc gtgccttcgg atcgctcgga
     8041 tatggccgag tggcaacaca atctggcggg acaggtctgt ggaatggggg cgcagaacgt
     8101 tgcggtgccg gtgacgtgga ccgggtatgc gtatgagggc gggttcgcga cgtgcgggcg
     8161 tgcggagatg atctccaaaa acgggatcgc ggagggcagt gaggtgccgg gtttggggca
     8221 aatcgatctg gctttttgtg cgcttcccga tgaccggaca acggtggtga tccagcgcgc
     8281 ggtcacccga aacccctgtt atctgctgcg ggtgcggggg ttgtcgctct cggtccccaa
     8341 cgatgcgctt aatggattca cgcggacact ccagagcgcg gctggccgcc gcgtgttgcg
     8401 ggcgttgccg gaccggtttg aaatcgtttc gccggaaggg cgctggttga atctggatgg
     8461 acggctgggt gtggtcgaac tgtacggcgg cgcgccgcat gtggcgcgtc tgcccgagcg
     8521 ccagattccg ctcaacattt ggaattccgc aggacccaag cgcggcgggg ggtatctgta
     8581 ttgtgaggag atcggacaga acatccgcga aggcgcggcc tatttcggcg cgcgggcgac
     8641 gatcttcgac tgcgcggcgg cggtggtggt cgcggatgcg gctgacacgg cggcaatcgc
     8701 ggcggaccgg gagacgggcg ccgttccctt agccgacgct gatctgcgcg cggtccgggt
     8761 tcgggatgct cggggcacgc tctggggcct cctcgcgaat ttttccacgg aggctaagtc
     8821 gttggattcg gaaccggtga gcggttggtt gggaagcggc gcggtcccgc tgcatgccgc
     8881 aacccatcaa atcgcgccgc acggcatcgc cgtttggcgg ctcgccggac agggtgacgt
     8941 gaccaccgct ggcgtgcggc cctagctgcg gagcgggtgg ggagtgcaca ggtcgaatgt
     9001 cgtggggcgc aaggcgacgt tcacgaggac gagaacggga aaacgtgaga aacagttccg
     9061 tttcgttacg gtgccacttg aagcggcccg accttcagca cctcgggcgg gggcaatgcg
     9121 ggaaaaggcg cgtgcggttc ggtctgatac cagtaagcca ccgaggccac atcgtcatgc
     9181 agcggcagat agcggccgga cttgtcccgc atccatccca gcgtctggat cgtcaccttc
     9241 aggtctttcg cgaaacggat cgggtcgacc acatgccagc ggtacagtcc gaagcgttgt
     9301 cccgtccgat agatcacgtc cggcggcagc acttgcgcca ggcccgagtg cggcgtgctg
     9361 aaggtctggt agcgtttcgt ttcccggttt tcgaaattgt aggagccgca gaaatagtcc
     9421 tccgtgccgg tgccgcagat cgtcgggaac gtctcgtcgc cgtctaggaa gaacttgatc
     9481 tcgccttcgc cccaccagcc ggggtcccgg acttcccatg cgagataggt gcccacatag
     9541 tgcccgtggc cgcgcacgcc gtccaggatc gtcacttcgg gacgctctcc tacgcgcggc
     9601 acgcgccgga actgcgcatg gaaggtcgcc gcgtcggcgg gaacatcggt cagcgtatag
     9661 ttgatctgat agtagaggcg catcgggttc tcatccagat tttcgagtgt aaccttggcg
     9721 cctttgcgga aaggcatgac ccagtagcag ttaaaggcgc tgccggggtt gacgcacacc
     9781 ggcgtcgagt tgatctggca atacttaccc catccgcacg cgaagaaatc accgagcggc
     9841 acctccaccg agggcgaggt ctcgccgtcc cagtagaagc gcaggatgtt gaggcgcgtt
     9901 ttgtcgagcg gcgcgggcgt catccaaatc tgctggatcg cgccggaacc ggcgatgtcc
     9961 gccagcgtga ccgtgctttt ggctggcacg ttcacgaacg gcgacacctt ccagccctgt
    10021 cccagatcgc gtgccgcgcg ccaagccggg ccgttggtcg actgcgctcc cgcgcccttc
    10081 gctcccgtga aattctcggg gctgatcgac ctcgtctcgg cttccgacaa gcggggcagc
    10141 gtgccgagac tcatatccag cccgctgaac ttcggtgtca cgcacgccgt gacccatccc
    10201 atcgcgatga acgccaatat acggactcga tgcatttttc actccttcat gattcgtcaa
    10261 tctgggacgt caggtcaaag atttttccgg ggttcatgat gtggttcgga tcccacgcgc
    10321 gcttgatggc gcgcatcaga ttcagttcga cgggatcgat gaggtcgctc agatacgctt
    10381 tccgcttgat gccgataccg tgctcgcccg aaatcttgcc gcccaaccgc cgggtaatcc
    10441 ggtacaggtc attcaggcag gccttttccg cagtgtgcca cgcctccatg ggcatgtccg
    10501 gatccttgac cagcgtggca tggaggttgc cgtcgccggc gtggccgtaa caggggatgc
    10561 gcataccgta tttcgcgctc aatttctcca gttcggggat gatctccgga atgcgggagg
    10621 gagggaccac gatgtcttcc agactctgga tcggactgta gactttgaag gcctcggcga
    10681 tgttacgccg cacgttccag atgcgctcta tggtattgcg gtcctcggcc acgaacacct
    10741 cgatcgcgcc gtgctcctcg cagaggtcgc cgatcgcgat cagatcctgc tccacctgcc
    10801 ccggatggga gccgtccgtc tcgatcagca gcatcgcgcc ggccgcttca tagggcaggc
    10861 tctcgttcag ataacggcaa ctggtgagga tcgagaggcg gtccatgaat tcgatgctgg
    10921 tgggcacgag acccttcgac atgatgatcg gcaccatgtc gatggcctcg cgcggggtcc
    10981 ggaaaagcac cagcagatcg gatttcgccg tagggtaacc gatgagccgg atggtggctt
    11041 gggtgacaat ccccaaggtg ccttcagaac cgatgacgag ttgtttgagg tcgtacccgg
    11101 tgacgtcctt cgcaagcttg ccgccaagcc atacgcactc gccggtcggc gtgaccagtt
    11161 ccagtccggt gacgtaacgg ccggtcacgc cgtatttgac cgctttgccg ccgcccgcgt
    11221 tctccgcgat attgccgccg atcatgcagg tttgcagact catcgggtat ccggcgaaaa
    11281 acaggccttt ttcctgcacc aacgcggcga ggtcattggt gaccatcccg gcctccacgg
    11341 tgacggtcat gttggcggtg tccaattcga tgaggcggtt catcttctcc agcgagatca
    11401 caatcccgcc gaactgcggg atggcgccac ccgacaggcc tgagcccgca ccacgggggg
    11461 tgatcgggat ccgctcgcgg ttggcaaggc ggaccactgc ggccacctgt tcggtcgagg
    11521 tcggaaacac cacgagttca ggcatgcggc catattcttc ggcgttggtc tcatcgtgcg
    11581 aatacgcctg aagcttttct tcatcggtca caacctgttt ggggccgacg atcgcgcgca
    11641 cggcatcgac gatcgcgggc gtgaccgggg cataagcggg ggtgggggag gaggtcatgt
    11701 tacgccttct ttcctttacg ggcttccagc cgccgctgca acgcgggcaa aatctgaaac
    11761 gcgtcgccca cgatcgcgta gtcggcgacc tgatgcagcg gggcatcggg gtcggggttc
    11821 acggatacga tgatctcact ggtcttgatc cccgcgaggt gctggatgga acccgagatg
    11881 cccgcgccga gatagagttt gggggagatg gttttaccgc ttaacccgat ctggtgggga
    11941 tagccgatcc agccgcggtc caccgcctcg cggcttgcgc cgaccacgcc gcccaacgtt
    12001 tcggcgagac gccgcagcat cgcgaaatgc tcgccctttt tcagtccttt gccgcccgcg
    12061 accacgatgt cggactcttc caagttcacg aagtccgaga cgtcccggcg gtaccccagc
    12121 acggtgacgc gcggatcgac caggctggcg tccagcggga tcgacatgat cgtgccgcgc
    12181 cgggtcgggt cggggggcag cggccgggtg gatttgggcc ggaccgtggc catctgcggg
    12241 cggtgatggg gggtcttgat ggtggccatg atgttgccgc cgatcgcggg ccgcgtctga
    12301 agcaggttgc cggtcccctc ctcgatgtcg agttcggtgc agtcggcagt gagaccggcg
    12361 cggacgcgca cggccacata cggcatcaag gtgcgccccg aagaggtggc ggccgccaga
    12421 atgatgtcgg gcttgcgctc gttaatcaaa tgggtcagga tccgtgcgta ggtctcgcaa
    12481 acgaaatggg cgagccgggg atcctgcacg gcgtagacct catccgcgcc gcgcgcgatc
    12541 aactcgtgca gacgtgaatc ctcgacgccg tccccgatga ggaccgaaac cagcggtact
    12601 ccgagctggt ccgcgagttt acgtccgcgt gccagcaatt cgaaagaaac ccctttgagt
    12661 tcgccgtgag cctgttcggc cagcgtccac actccactca ttgccgttct ccttcaatca
    12721 atcctttttc gtcaaggaac gcgatcaacc cgtccacggc cgcttccacg gcaccgtcgt
    12781 ccgttgccag cacctttcgc ccgccccggg tcactttggg cgtattgatc cggaccacct
    12841 tggtggggga acccttgagg ccgatgcacc cggagtcgag cggcaggttg gcggtgctga
    12901 aaaccggaat ctccatacgc atggccgcct ttttaccgcg tagggtcggc agccgcggcg
    12961 cggcaatctc cttcaccacg gtcaacaggc aggggagggg cacggacaac tgttggtatc
    13021 cctcctccac cagacgttcg agcgtcagcc tgcccttcgc gattccctcg attcgggcga
    13081 cgtacgtggc aacggggaga tcgagccaag ccgcgattcc cggtccgact tgggcggtgt
    13141 cgccgtccgt ggcgcgttcg cctgtgagaa tcaggtcgaa ggcccccata tgcaggatgg
    13201 cctgggcgag cgtgtacgaa gtggcccaag tgtcggcacc gccgaacgcg cggtcggaga
    13261 caagcgcggc attgtcgcaa cccatggcca ccgcttcttt cagcgctttg actgcgggcg
    13321 gcggccccat ggtgatggcg gtgacggttc ccccgtactg ctccttgagg cgcagggctg
    13381 cctccagcgc gtacaagtcc agcggattca ccacggtttc gatcccggcc cgaatcacgg
    13441 ttccggtttc cggatccatt ttgacgttac tggtttcggg gacctgtttg atcggcacga
    13501 taattttcac aagactctcc agaaaacggg ttaaacggag cataacaaaa accagattga
    13561 agacaatccg gattatcggc ttgtaccatt gcgcggggag gattactatt agacttttcg
    13621 ggaaaccgga gaggaagacg gtatgattat tcgcacgaca gcctatccgc gggccgcgct
    13681 gatcggcaac ccgtcagacg gatactacgg gaagacgatc gcgttcacct tttcaaattt
    13741 ttcggcgcgg gtgatcctgt acgagtcgcc ggaactggag attatgccga cgcggcgcga
    13801 cggctccgtc ttcacaagca tcggcgacct gttccaagac gtacgcctgt tcggctatta
    13861 tggcggaatc cggctcttga aagcggccat caaaagcttt tacgagtact gccgcgagaa
    13921 cgagattgaa ctgcatgacc gtaacttcac cgtgcgctat gacaccgaca tccccaatct
    13981 ggtcgggctc gccggttcga gcgcgatcat caccgccacc atgcgtgcgc tgatggcctt
    14041 ttacggtgtg accatcgcca agccccggct cgccaacctg atccgcgccg tggagaaccg
    14101 ggagttgggg atcgccgctg gcttgcaaga ccgcgtggcg caagtctatc agggccttgt
    14161 gtacatggat ttcgaccagg cgctgatgga gcgtcagggc ttcggacggt acgagccgct
    14221 cgacgtggcg accctgccgc cgctttatat cgcctttcgt acggatcttt ccgaaggctc
    14281 cgaggtggtc cacaacaacc tgcacgaccg gttcgagcgc ggcgaacccg aggtgctcgc
    14341 cgcgatgacg tattgggccg acctcacggc acaggcgcgg caggccttgg aaaagggtga
    14401 aggcggggaa ctcggaaggc tgatgaacgc caatttcgac aagcgccgcg agatctgcgc
    14461 catcggcgaa ggaaacctgc gtctggtgga aaccgcgcgt caggtcggcg cctcagccaa
    14521 attcaccggg tcgggcggcg cgatcatcgg cacctatcct gatgagcgta cctacacgcg
    14581 cctcaccgaa gtgttcgcgg cccagcaggt ctgcgtcttc aagccagata tcctctaaca
    14641 cgggggttca cgtcatgatc acgaaagcgg ttattcccgc cgccggtttc ggcacgcggt
    14701 ttctgccggc gaccaaagcc ttgcccaaag agatgttgcc gatcctcgac acgccggtca
    14761 tccagtatgt ggtcgaagaa gcggtggcct ccgggatcac cgacatcgtg atggtgatcg
    14821 gccggggcaa gcgggcggtg gaagagcact tcgaccgcag tttcgaactg gaggcggaac
    14881 tggaggccaa ggggcgcctg cgcgagttgg aaacgatccg cgcgatttct gaactcgcca
    14941 acatccactt tgtctggcag aagcgtatgc tgggcctcgg cgatgcgatc cgttgcgcgc
    15001 gataccatgt cggggaccaa ccgttcgcgg tgctgttggg agacacgctg cttcagagtc
    15061 tgagcggcgt tccggccacc cgacagctca tggaggcgca gtcgcgtcac ggcggctgcg
    15121 tggtggcggt ggaggaagtg ccgcgggagc gcgtcagcca gtacgggatc atcagcggcg
    15181 aaccgcttga cgcggagacg atccggattc gcgaccttgt ggaaaaacct gccccggacg
    15241 aggcgccctc gcaatgggcg gttgccagcc ggtatatcct gacgcccgac attttcgcgc
    15301 gtattgacgc cgcgacaccc ggccggaacg gtgagattca gatcaccgac gccctgcggg
    15361 cacgcgcact cgagggagag ccgctgttcg gcttccgcgt ggctgcgcgc cgccacgaca
    15421 tcggcaatat gctgggattc ctcaccgcca acatccggta tgcgttgggc catgcggaac
    15481 tggccgggcc gctcggcgcg ttcatcctcg ccgaggccga agcaatccgc cgcgcgcgcg
    15541 gcgaatgatg gcacaaaaaa aacggatgcc cggacaaggg agtccagcat ccgtttttcg
    15601 cgataaattc gcggaacgct tatttcttca gcgcgccatc gagcttcttc tgcagatcgc
    15661 cggcagcctt ttcggtgtcc ttcgctgcgg tgttggcgct cttttcggcg gatttgacgg
    15721 ctttgtccat cttatcgccc agagactcct ctttcttgca accgacaaac gcgcatgtcg
    15781 cggcggccat tacggcaatc ataagcaatt gcttcatctt gttgtactcc ttcttctttg
    15841 cgcggattgc gcaaggtaac acgtttacgt gtgtgtgcga tactatagca ggatcggggg
    15901 ggggctgaca atggggatat tttctagaca aaacaccgca ctgtcccgac agagttctcc
    15961 acggtcacga acggcactac ggtttaccct aaaaggggca ggtgaacggg tagaattcta
    16021 gcaaagcccc gtaaacattg ggttttgtcg cggtcctcgc cgcaccgtat tgagatgcgg
    16081 gcggagcccg caatctcaaa ggctgtgaga ctgtgaggct gttagactgt gaggctgtga
    16141 gactgtgagg ctgttaggct gtgaggcggt ggtgttccgt ggttaaatga tcgatcccga
    16201 tcccgatagc gatcccgacc ccgatgaaac catgaaacca tgaaaccatg aaacccagaa
    16261 acccagaaac ccagaaacca tgaaaccatg aaaccttgaa accagaaacc aaagagagtt
    16321 gaaggtgaag agttgaaacg gctacgccga tcgcgggacc gatgtcgcga tcttccccgg
    16381 cagggtaaaa acaaagccgc ccccgttagg gaacggcttc gcacagtcta aacggatccg
    16441 caaacagcgg atccatacgg acctaccaac gggactcgcg acgctcgccg ccgcggccgc
    16501 ctgcgccgcc gcgacccgag ccaccacgga aacccccgcc gccccccgcg ccgccgcgga
    16561 aaccgccgcc gcgctcttca cgcggtttcg gctgcgactc gttcacgcgg atggtacggc
    16621 cgtccagttc tttgccgttg agggcatcga ttgccgcctg cgcctgagtg cggtccggca
    16681 tctccacgaa accaaacccc ttggaacggc ctgtcatgcg gtcggaaaca acacgggctg
    16741 aagtcacctc gccgtaagct tcgaacgcaa ccttcaggga ctcatcgctc gtcgtatagg
    16801 caagattgcc cacatagatg tccatgtctc tatccttctc tacttcactc gcgccatgtt
    16861 gtgttcgtgt gtcaggcgca accggacccc atggctgatc cagcttcgta ggcagcctcg
    16921 cctatgtcgc acggcccgta acaacgggct aaagcgaccc caaagggagt gtgtatcaaa
    16981 aacgcgtttc gcctgaagtc aggcgtttcc tccatcgcga tgagccgaaa accatggaat
    17041 cagagacaag atcaaacagc taattcgcaa ttgacgaggg aaaaatagcg tatcgtggat
    17101 cgaatgtcaa ttgcgttttt agaaggcgta tccctcgatt ccctggccgc ttgaggtttc
    17161 gggtttcggt ggttttgggg ggcatggctc gaggggggag tcgctggtcg caagtcgacc
    17221 ttcatgcgtc ccttccgtta aggcatctca tttctcttcg atggacgatt cgatgaaacg
    17281 caggttcacg cataacggaa ccggctccgt attgtccgag gtgaaggaca cgcccacggt
    17341 gtcacccttt gcgaacgacg cgcgcggaat atcctcaagg gccacctcgc gcagcagttc
    17401 gtggcgcgcg ttatagacga ccgccagcga tacccggtgc gggatcgtca catactcgcc
    17461 aggcgcaagc gtggtcttca cgcggatctt gccgcggcgg ttgccccagc ggtagtgcgt
    17521 caccggattg accaccggac tggcgttttc gggcgcacgc atgataaaaa gcggcacggt
    17581 catcgcccat ttacggttgt cgatcggcga cacaacctcg aaatccgccg gttttaccga
    17641 cacctttgct atgaggtcgt ccgtgatgca ccagcgttcg cactgcccgg aggcgtggac
    17701 ccacttccgg ttgatcgagc gcggcatcac ggcggtgcct gttccgagtg ccttgaccgg
    17761 cgctgcggag gcgggcagtt cctcgaaccg ctcgcccttc catctgcgca caaacgagag
    17821 cgtaaacttt ccgtcctccc gcttcagttc ccacagcgtg tccgtctggc gcatgagccg
    17881 cttttcggtt tcggtgaacg cgctggcacg gcgggcatcc tcccacaggc ggatgcgctc
    17941 gcacttctcg taggttttcg gcaggccgaa ctggaagtcc acgcccgcat cgtgaccggc
    18001 ggcgaacgcc atgatccagt
//
LOCUS       DAOSLE010000049        17975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3347_length_17975_cov_8.552902, whole genome shotgun sequence.
ACCESSION   DAOSLE010000049 DAOSLE010000000
VERSION     DAOSLE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17975
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3347_length_17975_cov_8.552902"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..>1396)
                     /locus_tag="PK576_05025"
     CDS             complement(103..>1396)
                     /locus_tag="PK576_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOE60988.1"
                     /translation="LHVTQRRRTAAQSAYRDLRARLQNPAAPPDATRVCPCRQGTDPA
                     ALTEHWPFLRGPEMSGRSPLPRFPVRWNGVTGESVVWKTELRHGGSSTPVVWSNLLFL
                     TSGNAEARSVMAHDTKTGSLLWHTEIPDGDKTGAALPDVMDETGLAASTPACDERHVY
                     AVFGTGDLVALDHTGKPVWQIFLGRPKNTYGHASSLVYCGRMLIVQWDQEEHGRILAL
                     DKSTGRTLWETPRSAGMSWSTPVIMPVCDAFLLLVHATQQTWGMDLATGAKRWQVEAV
                     GGEVAPSLTWEGDVWLAANCYSKMVAFRLPPQGPPEKLWEWEDGNLPDVASPVILDGL
                     VYLANDAGEVFCHDLTNGKQVWLKEFEDGFYASPVAAGGHVYVVDRVKGVFRVFAAGR
                     EGRNVSTNPMGEAVSATPAFARGHIFVRGRKHLWAIGM"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1407..1846)
                     /locus_tag="PK576_05030"
     CDS             complement(<1407..1846)
                     /locus_tag="PK576_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60989.1"
                     /translation="MEARNRMLRQSARGIGFVALVFSLTVAALLTADATRSGSAETVR
                     AETLQRTLAASRTTPTDPDAVAFARELDRLARRAYFNSLTFRQTGMLLLAVGLILTAA
                     GFGVAWRLTLLIPDPRTLGESDPARTDRMAVNAVLATGGVLLLCA"
     gene            1999..3459
                     /locus_tag="PK576_05035"
     CDS             1999..3459
                     /locus_tag="PK576_05035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE60990.1"
                     /translation="MSIRNEVPTQEDIAKIDPLALSHMRPDWSASQPITLAQKRWLFA
                     VVAGVAVAALVWPLRTAQVLIAVCTALYVVIMLYKFIVIRASLSPSALLRFSPQDLVA
                     CEPREWPVFSVLVPMYKEPETVKQMVASLEALDYPADKKDVQILLEADDDATCEAARA
                     LSMPQGFRLTVVPPSFPRTKPKACNVGLALARGKYLVIYDAEDLPERDQLKKAVLAFE
                     SSPDNVVCIQSRLNYYNPRQNTLTRWFTAEYSAWFDLQLPGLAALGAVIPLGGTSNHF
                     RTEVLRELLGWDAYNVTEDCDLGVRLARAGYATRMLETTTWEEACSRPAFWIRQRTRW
                     QKGYIQTWFVHMRRPLALLRELGVVNFLHYQLLIGGVTFSVLVNPVFWMMTLVWFVFR
                     PEGVAQLFPGPVFAAGAVCLFLGNFVFLYVNLLGCCKRRQDSLMWWALLTPFYWMMMS
                     YSGWRAVIQFFRDPFVWEKTQHGLGSPAGPAAVRRG"
     gene            3503..5002
                     /locus_tag="PK576_05040"
     CDS             3503..5002
                     /locus_tag="PK576_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60991.1"
                     /translation="MTEDRKTYRWVRMLTGGVTPFVLAVALLVFVRVYVSACVPGAQE
                     AGICRELLAGTTVGRQALFGSCWVAPLPVLFYLPFAWIFPEPLAGMGAFFTAWFLVLW
                     AVREAVKATGQSGVRIVLAQTAVVGMVMLSPQAQTLGIRTALMTLFMLLGGAALADWA
                     AYRRIRDVVTAGWAGALLALCGLPAFVSAGVAVGMLPLAACGKRESRGRIQAWVLLGG
                     LPLVYTVGVWILMNRLVLGDPFFFVRSLSYLLPQPGVFVLAVLGSGLALLPALIMTWV
                     CDARTPEAPGAGPVAASAVLISFAVELLVCGWVLTRFGVGWHVGTLYVAALAVLVVAL
                     ARLRQSPVRLAVALGLFVCLSRFWLGGAPVSELSAGAENADADIPRQVEAFVESRTPY
                     GRVFVLGYAGLDLLRGYTGGRLQPNLDLHVGALRRAYPGQNLYVLVPRPTGAARSESV
                     FWKYPDLYVLGGDRLLYAATFGTWRLFEVVTAPTQEQWDEWRRAAESGN"
     gene            5040..5798
                     /locus_tag="PK576_05045"
     CDS             5040..5798
                     /locus_tag="PK576_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HOE60992.1"
                     /translation="MKRESQVLAGHVAPFALWIGVLLLLQGLEAVGMCPRGLYPWSYA
                     LKTVACAALFLWFAPWRVYPALKKNQVLPALLAGAAVAGVWIVPETPWMTRVAPAFQA
                     FYHRWLILFPGTLPDFVDPAWFPALPPGHRALSYAPEQAGWGLTVCRLVGSAGVIAVI
                     EEFFFRGFFYRWLRQNRFTEVPLTVWDAQSFWIVAVVFALEHDRWAAGLLAGVVYGWL
                     TVRTGSVWAAALAHGFTNLLLGVYVIVSGQYGFW"
     gene            5829..6530
                     /locus_tag="PK576_05050"
     CDS             5829..6530
                     /locus_tag="PK576_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60993.1"
                     /translation="MKRAEAKLNREYALRLLGVGALMIGLSLWSLYDGFVAWPRQNRA
                     LDVARSALLAQGLSAEAWLDRDAEGTRPIDAVFAAQGRRAPSKLVKKLGELRVPAAAA
                     DKERVRAMQTEQVRRLFEGPVYDTRDLRSQSIQAAVTLGLALLALLTVARKARLQFAA
                     DDSGLSGSGFGNVSIPYTELARIDWSKWDTKGIVVLTLRNGRRHQLDAWHFAGMTGLA
                     EALRQHRPDLAPDTD"
     gene            complement(6562..8994)
                     /locus_tag="PK576_05055"
     CDS             complement(6562..8994)
                     /locus_tag="PK576_05055"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOE60994.1"
                     /translation="MSVEGLKEDYAWHMRYTLAKEMENASPFDQYTAFAYCVRDRIIE
                     RWIETIACHRNQNVKRVYYLSLEFLIGRLLGNNVINLKMDEICREALADYGIDWHALR
                     DIEVDAGLGNGGLGRLAACFLDSMSTLNLPCVGYGLRYDYGIFKQRIVDGQQVEEPDH
                     WLKEGSPWEVARPENTQIVNFGGRVECITNGRIEWRWVETEQVIGIPYDLPIVGYGDT
                     VNTLRLWSARASDEFHLDDFNKGSYVEAVENKVLAENLTKVLYPNDNVLAGKELRLRQ
                     QYFFVSCSLQDILQRFRLKNSDWRLLPEKAFIQLNETHPALIIPELMRLLVDREGLDW
                     DTAWSITRTCTGYTNHTVLPEALEKWSVALMERLLPRHMQIIYEINGRFLRQIASLWP
                     GDMRRLARMSLIDEQGERSVRMANLAIVGSSAVNGVARLHSEILKTSLFRDFYELNPK
                     AFSNKTNGITQRRWLLKANPQLATLITEAIGDGWIVDLERLRELEGRLDDTAFLAAFR
                     KVKQANKEMLASHIHATVGIEVSPEAIFDVQVKRLHEYKRQLLLALYIIVLYNRLVED
                     PKYKPFPRVFLFAAKAAPGYLTAKLIIRLIHGIADVVNQNPHIRERLSVVFLPDYRVS
                     LAEKIIPAANVSEQISLAGTEASGTGNMKLMLNGALTLGTLDGANVEIHEAVGDENIF
                     LFGLRAEQVNELRPVYSSQAIYHADPEIRHAIDMIRHNVFSLLTPGIFDPLIRSLLDY
                     NDQYMLLADLRDYIETQDRVEALYRKPQEWDRKALINVARSGWFSSDRTIREYARDIW
                     HVEPVIPAQPES"
     gene            9516..12482
                     /locus_tag="PK576_05060"
     CDS             9516..12482
                     /locus_tag="PK576_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOE60995.1"
                     /translation="MKQLRVLTCAVGLALMAAMSFAGGYRLKYIATGWDIGRATPEQI
                     LKMMPKFEQTAFDGVAIQCSAPQDDALLDAGYIFNDPAWRYESFEKLVETYRAINSRG
                     KLKENFISAGLSARNTERNLAKHGGRVRLDDDAAWARIAHNYGMLAKIAKAGGFRGIQ
                     LDNEDYHGLHQFEFRPGDGDYEAAAEKMRQRGREVCGAIFREFPDVRLGFFWAFSSVR
                     RQVQAPDPLKAIRESNRLFPQFLNGLLDVMPPEAVVIDFDEDAYTFRGADGTFERTFV
                     HMMKHALVAVAPENRAKYRAQFRNSFLLYLDQYVGSKYRVRAGKRGQPNNWYRAPVNG
                     SRAGAFYADLEAAANAADGYLGIYGERFGFVDWEHELDAAMKPWNALLSRITWDEELG
                     LDAKLRLVRDPHAFMLEELAAAKKDPARWNLLETLPNFGHARPGTFIVHEEVTNRNYF
                     AVTFESKGETAAVARWQNGLAWNWEPGGVRHVAFTKEGVSGDGWSLYTALLRVPVAAT
                     RLQLQIGTNEVRNAAMYRLDPPPTRPFVLAYQLDISRGKVPTLPNLKRIVDTIASLGY
                     TQFQLYMECAFAYKGYEAAWKDRSPMTPDEMRELSTYCRSKGMDLVPSQNTFAHMGPW
                     FEVADLRKRLAECPDGANIEAPKFKKKRGPVTLVASGGESLKFLSDLFDQLLPCAESR
                     YVNIGCDEVYDLWDKNCRSAAKVAKDGYARTYFDHVLAVRKLAYQRQRQSMFWADAIF
                     EYPELIREIPKDMIALVYGYTAGGGDKFDEKCAALAADEVRFYVCPGTSGWCGPSGRT
                     KNMLGNVKEACEAGRRHGAEGLMVCDWGDGGHHQPWIVALPSLVYAAGLVQGKDLTRA
                     EIAAKVDAICGVAVGAALIRYGDVYETTPHLRYLYHCYSTPQSEKIGPESLAVVREAK
                     SLAELEKAPTWVKEDFATIDFLYGLIEAHAQGKVPDAKSLEEDYRTLWLKQNRPGGFE
                     NSLRFLKEKPHETQRR"
     gene            12502..13323
                     /locus_tag="PK576_05065"
     CDS             12502..13323
                     /locus_tag="PK576_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE60996.1"
                     /translation="MTKMKWLTGIAMMLACGAVADAECNGTVPPKRMTIVEELFAPHS
                     VILFQGDSITHGGRLRDMNHYLGHGYQAEIAMRYLAYRPDLRLQFGNRGVSGHTSSNL
                     VARWHHDAVPYTPDESGYGGVFPGERNKPRVPDVLSILIGINDYLRKSDGWFVPVADY
                     ERNLRFMVTNSLAANPKMKIVLCEPFRLPEDTNPDFLARQATVRRVATDYNLVFVPFQ
                     KLFSEELLKEEPRPRYWFWDAFHPTYAAHMRMADFWIATVAREFASGKGNPSLRR"
     gene            13734..14024
                     /locus_tag="PK576_05070"
     CDS             13734..14024
                     /locus_tag="PK576_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOE60997.1"
                     /translation="MPTSRLLQVVGMSDSTLRRRRVSGRLDQNESERVYRLGKLFHLA
                     TRVLGSRELAGQWFKMPATALSGSTPLDYADTEIGKREVEKMLYRIDSRLLK"
     gene            14183..15325
                     /locus_tag="PK576_05075"
     CDS             14183..15325
                     /locus_tag="PK576_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOE60998.1"
                     /translation="MDFLLDGIRAFTWQQAVMFAVGFTLIWLAIKKQCEPMLLLPMGF
                     GAILVNIPFSGALNQVLPGVGETQGAIQWLFEVGIESSEILPLLLFIGIGAMIDFSPL
                     FQRPIYFVFAAAAHFGIFATIVLACVLGFDLRDAAAIGIIGAADGPTTILVAQVLQSK
                     YMGAIAVTAYSYMALVPIIQPAVIYLLTSRDERRIRMEPTKDSDIPRTVRILFPIAVT
                     IVVGLISPAAVSLIGFLMFGNLIRECGVLESLSRTAQNELTNLITLLLGIIIAFTMKA
                     DQFVTRDTIMILGLGLFAFVCDTAAGLLFAKFLNLFLRKKINPMIGATGISAFPMSAR
                     VIHKLAMKEDPGNILIMQAVSANVAGQIASVAASGLVLSLVLKYLQ"
     gene            15346..15498
                     /locus_tag="PK576_05080"
     CDS             15346..15498
                     /locus_tag="PK576_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE60999.1"
                     /translation="MEHAENVRISLTIMWQGMLGLFIITIVMAWLTSTIIKFCKRPEN
                     KETPSD"
     gene            15930..17567
                     /locus_tag="PK576_05085"
     CDS             15930..17567
                     /locus_tag="PK576_05085"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOE61000.1"
                     /translation="MKISFPSKGITERTSRTRTGGRISAIIFGLIFGTVGLVMLVLAV
                     RSSLRQLDTRHWIATPCEIVESSITRDDEQHRFDTVFTYSFNGHSYTSRRFTHEGGLR
                     EKHINDAQRLLSQYPQGTSTTCYVNPAAPDDALIRRDLSVGGMIVPILLALPLVLSGY
                     GLIFFVWHRNRPEPPTSDFSGGKPSRGTMAVSIRFGTIFIVAGLGVTYFTFLGPFLRQ
                     CAARDWTPVEAVVEQSRVRSHSSDEDTTYYSVDITYCYRIDGRDYRGDRYNFNTGSSS
                     DYEGKQRVVAAHPPGSTLFVYVNPADPFDSVIVRDMGATLYLGLLPLIFTVIGAMALI
                     SGILRQRRRLAHPLAPPSAWTLCPAFRAGRPLWVLAFALFWNGATSVFVVQCLREWQK
                     GGRPIFLTFFLVPFVLIGAGCVVGFFVELLRLFNPRIILIPPPDPLVPGAPTMIAYRI
                     RGRIDRVTRLTISLVGSGVAKEHGDDTAASRELHRLVVYEQEQPMLMQEGLFRLTLPP
                     ELMGRGADPHNPITWHLEVKGEIPRGLDIVETYRLIS"
BASE COUNT         3233 a         5096 c         5823 g         3813 t
ORIGIN      
        1 gcctgcggga gagtaggacg ccgccgggag tttttttccg gggggccgcg gatcgtcccg
       61 atccgcggcc cccccttttt ttgcccgagc tttcggacta cattacatcc cgatggccca
      121 gaggtgcttc cgaccccgta cgaaaatgtg tccgcgggca aaggccgggg tggcgctgac
      181 agcctcgccc atggggttgg tcgaaacatt ccggccttcg cggcccgccg caaagacgcg
      241 gaaaacacct ttgacgcgat ccaccacata cacatgaccg cctgcggcaa ccggtgaagc
      301 gtagaagccg tcctcaaact ctttgagcca gacctgtttc ccgttggtca gatcgtggca
      361 gaatacctcg cccgcatcat tggccagata caccagaccg tcgaggatga ccggactggc
      421 cacatcgggc aggttgccgt cctcccactc ccagagtttt tccggcggac cctgcggcgg
      481 aagacggaag gcgaccatct ttgaataaca gttcgcggcc agccagacat caccctccca
      541 tgttagcgag ggcgccacct cgcccccgac cgcctcgacc tgccaccgct tcgcgccggt
      601 cgccaaatcc attccccagg tctgctgcgt cgcatgcacc aacagaagga acgcgtcaca
      661 gaccggcatg atcacgggcg tgctccagga catgcccgct gagcgcgggg tctcccatag
      721 agtacgcccg gtcgatttgt ccaacgccaa gatgcggccg tgctcctctt ggtcccactg
      781 cacaatcagc atgcgtccgc aatagacgag cgaggaggca tgtccatagg tgtttttcgg
      841 acgacccaga aaaatctgcc acaccggttt gccggtgtga tccagcgcca cgaggtcccc
      901 ggtgccgaac accgcgtaca catggcgctc atcgcaggcc ggggtcgatg cggccaagcc
      961 ggtctcatcc atcacatccg gcagtgctgc gcccgttttg tctccatccg gaatctcggt
     1021 gtgccacagc agggatccgg ttttcgtatc atgcgccatc acactgcgtg cctcggcatt
     1081 tccggaagtc agaaaaagga ggttgctcca gaccaccggt gtgctggagc cgccgtgtcg
     1141 taactccgtc ttccagacga cgctttcgcc ggtgacgccg ttccagcgta ccgggaagcg
     1201 ggggaggggg gagcggcccg acatctccgg accccgcaga aagggccagt gctctgtaag
     1261 cgcggccgga tcggtgcctt ggcgacaggg acagacgcgc gtcgcgtcgg ggggggctgc
     1321 cggattttgc aggcgggcgc gcaggtcacg gtaagcggat tgggcggcag tgcggcggcg
     1381 ctgggtcaca tgcagcnnnn nnnnnngcgc aaagcagtaa tacgccaccg gtcgccagaa
     1441 ccgcgttgac tgccatgcgg tctgttcggg cgggatcgga ttcccctaat gtgcgcgggt
     1501 ccgggatcag cagggtcaga cgccaggcga caccgaaacc ggccgcagtg aggattaagc
     1561 cgacggccaa gagaagcatg cccgtctgac ggaaggtcag gctgttgaaa taggcgcgtc
     1621 gcgcgagacg atccaactcg cgcgcgaacg ctacggcatc cggatccgtc ggggtggtgc
     1681 ggctcgccgc cagtgtccgt tgcagcgttt cggcacgcac ggtctccgct gaaccggagc
     1741 gggtagcgtc cgcggtcagc agggccgcga ccgtaagact gaagaccagt gccacgaacc
     1801 cgataccgcg cgcactttgc cgcagcatcc ggttccgtgc ttccatgcct gtgcacctca
     1861 tgccgagttc tcccttcgcg gttccaactt tattcggtat cttagcggag gcggaaaggt
     1921 gtggcaacac tcttcctctt gtgagggacg tgtggtacag tgatacgggg atggggtttg
     1981 ggggagggat atcgaagcat gagcatcagg aacgaagtgc cgacgcagga agacatcgcc
     2041 aagattgacc cgctggcgct gtcgcacatg cggccggatt ggagtgcgtc gcagccgata
     2101 acgcttgcgc agaagcgctg gctgttcgcg gtggttgcgg gtgtggctgt tgcggctttg
     2161 gtgtggccgt tgcggacggc acaggtgctg atcgcggtct gtaccgcgct gtatgtcgtg
     2221 atcatgctgt acaaattcat tgtgatccgc gcgtcgctgt cgccctctgc gctgttgcgc
     2281 ttctcgccgc aggatctggt cgcgtgcgag cctcgggaat ggccggtttt ctccgtgctg
     2341 gtgcccatgt acaaagagcc ggagacagtc aagcagatgg tggcgtcgct ggaggctttg
     2401 gattatccgg cggacaagaa ggacgtgcag attctgctgg aggcggacga tgacgccacg
     2461 tgcgaggccg cgcgtgccct ctccatgccc caagggttcc ggctgacggt cgtgccgccg
     2521 tcttttccgc ggaccaagcc caaagcgtgc aatgtgggct tggcgttggc ccggggcaaa
     2581 tatctcgtga tttatgatgc ggaggacctc ccggaacggg accagctcaa aaaagcggtt
     2641 ctggccttcg agtcctcgcc ggataatgtg gtctgtatcc aatcgcgcct caattattac
     2701 aacccccgtc agaatacgct gacgcgctgg ttcacggccg agtactccgc gtggttcgac
     2761 cttcaacttc cggggttggc ggcactcggc gcggtcattc cgttgggcgg cacctccaat
     2821 cattttcgga cggaggtgct gcgggagttg ctgggatggg acgcctacaa tgtcacggag
     2881 gattgcgatc tgggggttcg gctggcacgc gcgggctacg ccacacgtat gctggaaacc
     2941 accacatggg aggaggcgtg cagccgcccc gcgttctgga tccggcaacg tacccgctgg
     3001 cagaaaggtt acatccagac gtggttcgta cacatgcggc ggccgctcgc gctgctgcgt
     3061 gaactggggg tggtgaattt cctgcattac cagttgctga ttgggggggt gaccttctcg
     3121 gtgctggtga atccggtgtt ttggatgatg acgctggtct ggtttgtgtt tcgcccggaa
     3181 ggggtcgccc aactcttccc cggtccggtt ttcgcggctg gcgcggtctg tctgttcttg
     3241 gggaatttcg ttttcctgta cgtgaatctg ctggggtgct gcaaacggag gcaggacagc
     3301 ctgatgtggt gggccttgct cacaccgttt tattggatga tgatgagtta cagcgggtgg
     3361 cgggcggtca tccagttttt ccgtgatccg tttgtgtggg agaagacgca gcatggtttg
     3421 gggtctccag ccggtcccgc ggcggtgcgg cggggctgag agcagaaagt gtggtggcgc
     3481 gtacgagaag gcagggtagg gtatgacaga ggatcggaaa acgtaccggt gggtccggat
     3541 gctgacaggc ggggtgacgc cgttcgtgct ggctgtggca ttgttggttt tcgtgcgggt
     3601 ttatgtgtcc gcgtgtgtgc cgggcgcgca ggaggcgggt atttgccgcg aattgctggc
     3661 cgggaccacg gtggggcggc aggcgttgtt cggttcatgc tgggtcgcgc cgttgccggt
     3721 gctcttttat ctgccgttcg cttggatctt tcccgagccg ttggccggga tgggcgcatt
     3781 tttcacggca tggtttctgg tgctgtgggc cgtgcgcgag gccgtcaagg cgaccgggca
     3841 gtcgggtgtg cggatcgtgc tggcgcagac cgccgtggtg gggatggtga tgctttcgcc
     3901 gcaggcgcag acgttgggga tccgcacggc gctgatgacc ctgttcatgc tgttgggggg
     3961 ggcggcgctg gcggattggg cggcgtaccg caggatccgt gatgtggtca ctgcgggctg
     4021 ggccggagcc ctgctcgcct tgtgcggatt gcccgccttt gtctcggccg gtgtggcggt
     4081 ggggatgttg ccgctggcgg cctgcgggaa gcgtgagtcg cgcggccgca tacaggcctg
     4141 ggtgctgctc ggcgggttgc cgttggtgta tacggtcggg gtctggattc tgatgaaccg
     4201 gctggttttg ggtgacccgt tcttttttgt gcggtccctg tcgtatctgc tgccgcagcc
     4261 cggcgtgttt gtgttggcgg tgctgggttc ggggctggcc ctgttgcccg ccctgatcat
     4321 gacgtgggtc tgcgatgcgc gcacgcctga ggcgcccggc gcgggtccgg tggcggcctc
     4381 cgctgtgctg atctcgtttg cggtggagct gttggtgtgt ggatgggtgc tgacgcgctt
     4441 cggtgtcggg tggcacgtcg ggacgctgta tgtcgcggcg ctggcggtgc tggttgtggc
     4501 tcttgcgcga ctgcggcagt cgcctgtccg cttggccgtc gcactcgggt tgttcgtgtg
     4561 cctgagtcgg ttctggctgg gcggcgcacc tgtgtcggaa ttgtcggcgg gtgcggaaaa
     4621 tgccgatgcc gacatcccgc ggcaagtcga ggcttttgtc gaatcccgca cgccgtacgg
     4681 ccgggtgttc gtgttgggat acgccgggct ggatctgttg cgcggttata cgggggggcg
     4741 gctccagccc aatctggacc tgcatgtggg cgcgttgcgc cgcgcgtatc ccggccagaa
     4801 tctctatgtg ctggtgccgc gtccgaccgg ggcggcgcgt tcggagagcg tgttttggaa
     4861 ataccccgat ttgtacgtat tgggcggaga ccggctgctg tatgccgcga ccttcggcac
     4921 ctggcggctt ttcgaggtgg tgaccgcgcc gacgcaggag cagtgggacg aatggcggcg
     4981 ggctgctgag agcggaaact gatgctgccg gggatagcgg gtaaaggagt gccgcgcggg
     5041 tgaaacggga gagtcaggtg ttggcgggac atgtcgcacc gttcgcgctt tggatcggcg
     5101 tgctgctgtt gctgcagggg ctggaggcgg tcgggatgtg cccgcgtggc ctatacccgt
     5161 ggagttatgc actcaaaacc gttgcctgtg ccgcgctgtt tttgtggttc gcgccttggc
     5221 gcgtctatcc ggccttgaaa aaaaaccagg tgctgcccgc actgcttgcg ggcgcggcgg
     5281 tggcgggggt ctggattgtg ccggagacgc cctggatgac gcgcgtggcg cccgcgtttc
     5341 aggcgtttta tcatcggtgg ctgattctgt ttccgggtac gttgccggac tttgttgacc
     5401 cggcgtggtt tccggcgctg ccgccgggac atcgagcgct ctcgtacgcg ccggagcagg
     5461 ccggatgggg tttgaccgta tgccgactgg tgggctcggc aggcgtcatc gcggtgatcg
     5521 aggagttttt tttccggggg tttttttacc gctggctccg ccagaaccgt ttcacggaag
     5581 tgccgctcac tgtctgggac gcgcagtcgt tttggattgt ggcggtggtg ttcgcgttgg
     5641 agcatgaccg gtgggccgca gggttgctcg caggggttgt ttacggatgg ttgacggtgc
     5701 ggaccggcag tgtctgggct gcagcgctgg cgcacgggtt caccaatctg ctgctcgggg
     5761 tgtatgtgat tgtgtcggga cagtacgggt tctggtgaag tgggtgtcgg gaaaaggacg
     5821 gagggaaagt gaagagggct gaggcgaagt tgaatcggga gtatgcgttg aggcttttgg
     5881 gtgtcggcgc gctgatgatc gggttgagcc tgtggtcgct gtacgacggc ttcgtggcct
     5941 ggcccaggca gaaccgtgcg ctggatgtgg cgcggtcggc gttgctggcc caaggtcttt
     6001 cagcggaagc ctggcttgat cgggatgcgg agggaacacg tccgatagat gccgtgtttg
     6061 ccgcgcaagg gaggcgcgcg ccttccaaat tggtcaaaaa gctgggagag ttgagagtcc
     6121 cggccgctgc cgctgataag gagagggtgc gcgcgatgca gactgagcag gtccgtcgat
     6181 tgttcgaggg gccggtatac gacacgcgcg accttcgatc gcaatccatt caggctgcgg
     6241 tgaccttggg tctggcgttg ctggctttgc tcacggtggc gcgcaaggcg cggctgcagt
     6301 ttgcggcgga tgattcgggc ctgagcggga gcggattcgg gaatgtgtca ataccgtata
     6361 cggaactcgc gcggatcgac tggtcgaagt gggacacgaa gggcatcgtg gttctgacgc
     6421 tccggaacgg acggcggcat cagttggacg catggcactt tgccgggatg acggggctgg
     6481 cggaggcact ccgccagcac cgcccggatc tcgctccgga cacggactga actcccgcgc
     6541 ttcgttgcgg agaccgcgcc gtcaggattc aggctgcgcg ggtatgaccg gttcgacgtg
     6601 ccagatgtca cgtgcgtatt cgcggatcgt ccggtcggag gaaaaccagc cggaacgggc
     6661 cacgttgatc agcgcctttc ggtcccactc ctgcggtttc cggtacagcg cttccaccct
     6721 gtcctgggtc tcgatgtagt cccgcagatc cgccaacagc atgtattggt cattgtagtc
     6781 gagcaaggac cggatcagcg gatcgaaaat gccgggcgtg agcagactga acacattatg
     6841 ccggatcatg tcgatcgcgt gacgaatctc cggatccgcg tggtagatcg cctgtgagga
     6901 atagacagga cgcaactcat tcacctgctc ggcccgcagg ccgaagagga agatgttttc
     6961 gtcgcccacc gcctcatgaa tctccacgtt ggcgccgtcc agcgtgccga gtgtcagggc
     7021 accgttgagc atgagtttca tgttgccggt gccggatgcc tcggtgccgg ccagagagat
     7081 ctgttcgctg acattggcgg cggggatgat cttttcggcc agcgacacac ggtaatcggg
     7141 caggaagacc acactcaggc gctcccggat atgcgggttc tggttcacca catccgcgat
     7201 accgtggatc aggcggatga tcagtttggc cgtgagataa ccgggcgcgg ctttggccgc
     7261 gaagagaaag accctcggga acggtttgta ctttggatcc tccaccagcc ggttgtacag
     7321 cacgatgatg tacagcgcga gcagcagttg gcgtttgtac tcgtgcaggc gcttgacctg
     7381 cacgtcgaag atcgcttcag gggagacctc gatcccgacc gttgcgtgga tatgacttgc
     7441 cagcatctcc ttgttcgcct gcttgacttt tctgaaggcg gcgaggaagg ctgtgtcgtc
     7501 aagacgcccc tccaactccc ggagccgctc gagatcaacg atccatccgt cgccaatcgc
     7561 ttcggtgatc agcgtcgcga gttggggatt ggccttaagc aaccagcgtc gttgggtgat
     7621 gccgttggtc ttgttggaga aagccttggg gttgagttcg taaaagtccc ggaagaggga
     7681 ggtcttcaaa atctccgagt ggaggcgcgc cacgccgttc actgcggagg agccgacgat
     7741 cgccagattg gccatccgaa cggaacgttc cccctgctcg tcgatcaagc tcatacgggc
     7801 cagacgacgc atgtctccgg gccacaggga cgcgatctgc cggaggaaac gcccgttgat
     7861 ctcgtagatg atctgcatgt gcctcggcaa cagacgctcc atcagcgcga cgctccactt
     7921 ctccagagcc tctggtagca cggtgtggtt cgtgtaaccg gtacaggtgc gggtgatcga
     7981 ccaggcggtg tcccagtcga gcccttcgcg gtccaccagc agacgcatca gttccgggat
     8041 gatcagcgcg ggatgggtct cgttgagttg gatgaaggct ttttcgggca gcagccgcca
     8101 gtcgctgttt ttgagacgga aacgctgcag gatatcctgc agggaacagg aaacgaagaa
     8161 atactgctgc cgcaaacgca gctctttgcc cgccagcaca ttgtcattgg ggtagaggac
     8221 cttggtgagg ttttcggcca gcactttgtt ttcgaccgcc tcgacgtacg accctttgtt
     8281 gaagtcatcg aggtgaaatt cgtcactggc ccgcgccgac cagagccgca gcgtgttcac
     8341 ggtgtcgccg tatccgacga tcggaagatc gtacgggatt ccgatgacct gttcggtctc
     8401 gacccagcgc cactcgatcc ggccgttggt gatgcactcg acccggccgc caaagttgac
     8461 gatctgggtg ttttccggcc gcgccacttc ccagggcgaa ccttctttga gccagtgatc
     8521 cggctcctcg acctgctggc cgtccacaat ccgctgtttg aagatgccgt agtcataacg
     8581 cagcccgtat cccacgcagg gcaggttgag cgtcgacatg gagtccagaa aacaggcggc
     8641 gagtcggccg agaccgccgt tgcccaaccc ggcgtcgacc tcaatgtcgc gcagggcgtg
     8701 ccagtcgatg ccgtaatccg caagcgcctc ccggcagatc tcgtccatct tcaggttgat
     8761 gacgttgttg ccgagcagac gcccgatcag aaattcgagg gagaggtaat agacgcgctt
     8821 gacgttttga ttgcggtggc aggcgatggt ttcgatccag cgttcgatga tacggtcgcg
     8881 cacacagtag gcaaaggccg tgtactggtc aaacggcgaa gcgttttcca tctccttggc
     8941 gagggtataa cgcatatgcc aggcatagtc ttccttgagc ccttcgacgc tcatttcaac
     9001 acggtggtca cgttgcgatt tgcgaggggg agtcttttga gccatagtgt ccttgtaatc
     9061 cttttgatcc ttaaaacgtt tccaatagta tgacagaaat ccggaagggc gtcacgttat
     9121 aaggtcagca aggcatacgg ggtttgggcg tatcctcgtt cccgtgaccg ttgaaatttt
     9181 ggtttcaggc gcctggtcca gacgggagcg cgggcgtccc cgcccgcaac aacgcgccgt
     9241 gcgccacaaa gcgggcaaga tgcccgcgct cccggctgcg catcaaagga ttcgatgatg
     9301 gctgctgatc ctgtcccgct ccttgggcga caccttggcg atcggaatcg aaagttcagc
     9361 gcagccaacg tcaacataca cgcggacggg gacgaggacg agtcgtgcgc attgactgat
     9421 ggtgggcaga cggtttgact ctttcggtcc ggttcggcaa taatgatcgc ggcgtggaat
     9481 aatactgcag ggtaatcatt tcgggacgag gggaaatgaa acagctcaga gtgttgacgt
     9541 gtgctgtggg gcttgcgctg atggcagcga tgtcctttgc gggcggatac aggctcaagt
     9601 acatcgccac cggctgggac atcgggcggg cgacgcccga gcagatactg aagatgatgc
     9661 cgaagttcga acagacggcc ttcgacggtg tggcgatcca gtgttcggcg ccgcaggacg
     9721 acgcgttgct ggatgcgggc tacatcttca acgatcccgc ttggcgctac gagtcgttcg
     9781 agaagttggt cgagacgtat cgggcgatca actcccgcgg caaactgaag gaaaacttca
     9841 tctctgcggg actctccgcg cgcaacaccg aacgcaatct tgcgaagcac ggcggccgcg
     9901 tccgtctcga cgacgacgca gcctgggcgc gtatcgccca caactacggc atgctcgcga
     9961 agattgcaaa ggcgggcggc ttccgcggca tccaactcga caacgaggat taccacggac
    10021 ttcatcagtt cgagtttcgc cccggtgacg gggattacga agctgcggcc gagaagatgc
    10081 ggcagcgcgg acgggaggtg tgcggtgcga tcttcaggga gttcccagac gtccggctcg
    10141 gtttcttctg ggcgttttcg agcgtccgtc ggcaggtgca ggcacctgat cctttgaagg
    10201 cgatccgcga atcgaaccgg cttttcccgc agttcttgaa cggactcctc gatgttatgc
    10261 cgcccgaggc ggtggtgatc gactttgacg aggacgccta caccttccgt ggcgcggacg
    10321 gaacattcga gaggacgttc gtccacatga tgaaacatgc gcttgtcgcc gtcgcgccgg
    10381 agaatcgtgc gaaataccgt gcgcagttcc gcaactcgtt ccttctctac ctcgaccagt
    10441 acgtgggctc caagtaccgc gtccgcgcgg gcaagcgcgg tcagccgaac aactggtacc
    10501 gcgcgcccgt aaacggttcg cgcgccgggg cgttctacgc cgatctcgaa gcggccgcaa
    10561 acgccgcgga cggatatttg ggcatctacg gcgagcgttt cggctttgtc gactgggagc
    10621 acgagctgga cgccgcgatg aagccgtgga acgctctcct ctcgcgcatc acatgggatg
    10681 aggaactcgg gctcgatgcg aaactccggc tcgtccgcga ccctcatgcg tttatgctcg
    10741 aagaactcgc tgcggcgaag aaggatcctg cgcggtggaa tctccttgag acgctgccta
    10801 acttcgggca tgcgcgtccc ggcacgttca tcgtccatga ggaggtgaca aaccgcaact
    10861 acttcgcggt gacttttgag tcaaagggcg agaccgctgc ggtcgcgcgc tggcagaacg
    10921 gactggcctg gaactgggaa ccggggggcg tccggcacgt cgcgtttaca aaagagggcg
    10981 tttccgggga cggctggtcg ctctacacgg cgcttctgcg cgttcctgtc gcggcgacgc
    11041 gtctccagtt gcagatcggg acgaacgagg tccgtaacgc cgcgatgtac cggcttgatc
    11101 caccgccgac gcgtcccttc gtgctcgctt accaactcga catctcgcgc ggcaaagtcc
    11161 cgacgttgcc gaatctgaag cgcatcgtcg acacaatcgc ctcactcggc tacacgcagt
    11221 ttcagctcta tatggagtgc gcgttcgcat acaaaggcta cgaggcggcg tggaaggacc
    11281 gcagccccat gacgcctgac gagatgcgcg agctttcaac gtactgccgg tcgaagggca
    11341 tggatctcgt tcccagccag aacacattcg cgcacatggg gccgtggttc gaggtcgccg
    11401 acttgcgcaa gcgccttgcg gagtgtccgg acggcgcgaa tatcgaggcg ccgaagttca
    11461 agaagaagcg cgggccggtc acgctcgtcg cctccggcgg cgaatccctc aagtttctca
    11521 gcgacctttt cgaccagctc ctgccgtgcg ccgagagtcg ctacgtgaac atcggctgcg
    11581 acgaggtata cgacctctgg gataaaaact gccgcagtgc ggcaaaggtc gcgaaagacg
    11641 gttatgcgcg tacctatttc gaccacgtgc tcgccgttcg gaagctcgcc taccagcgcc
    11701 agcgccaatc catgttctgg gcagacgcga ttttcgagta tcccgagttg atccgcgaga
    11761 tccccaagga catgatcgcg cttgtctacg gctacacggc gggcggcggc gacaagttcg
    11821 atgagaagtg cgcggcgctc gcggctgacg aggtccgctt ctacgtctgt ccggggacga
    11881 gcggctggtg tggtccctcg ggccgcacga agaacatgtt gggcaatgtg aaggaggcgt
    11941 gcgaggcggg gcggcgtcat ggtgcggaag gcctgatggt ctgcgactgg ggcgacggcg
    12001 gacaccatca gccgtggatt gtggcgcttc cgtcgcttgt ctatgcggcg ggccttgtgc
    12061 agggcaagga cctgacgcgc gccgaaatcg ccgcgaaggt cgatgcgatc tgcggggttg
    12121 cggtcggggc ggcgctcata cggtacgggg acgtctacga gacaacaccg cacctgcggt
    12181 atctttacca ctgctacagc actccgcagt ccgagaagat cgggccggaa tctcttgccg
    12241 tcgttcggga ggcgaagtca cttgccgaac tcgaaaaagc gccgacttgg gtgaaagagg
    12301 atttcgcgac catcgacttc ctctacggcc tcattgaagc gcatgcgcag ggcaaggtgc
    12361 ccgatgcgaa gtctctggaa gaggattatc gcacgctttg gctcaagcag aaccggccgg
    12421 gcggttttga gaattcgctc agattcctca aggaaaaacc acacgaaact cagaggcgct
    12481 gattagaagg agtatgcaga catgacgaag atgaagtggt tgactggcat cgcgatgatg
    12541 ttggcgtgcg gtgcggtggc tgacgcggaa tgcaacggca cggtcccgcc taagcggatg
    12601 acgattgtcg aggagctgtt cgcaccccat tcggtgattc ttttccaagg tgattccatt
    12661 acgcacggcg ggcgcttgcg cgatatgaac cactatctgg gtcacggtta tcaggcggaa
    12721 atcgccatgc gctatttagc ctaccgcccc gatctgcgtc ttcagttcgg taatcgcggc
    12781 gtcagcgggc acacgagttc caatcttgtg gcgcgctggc atcatgacgc ggttccgtac
    12841 acgccggacg aaagcggtta tggcggtgtg ttcccgggcg agcggaacaa gccgcgtgtg
    12901 ccggacgtac tgtcgatcct gatcggcatc aacgactacc tgcgcaagag cgacggctgg
    12961 tttgtgcctg ttgccgacta cgaacgaaat ttgcgcttca tggtgacgaa ttctctcgcg
    13021 gcaaatccga aaatgaagat cgtgttgtgc gaaccgtttc gtctgccaga ggacaccaat
    13081 cctgattttc ttgcgcgcca agcgaccgtg cggcgcgtgg cgacggacta caatctggtg
    13141 tttgtgccgt ttcaaaagct gttctcagaa gagctgctca aggaagaacc gcggccgcgc
    13201 tactggttct gggatgcgtt tcatcccacg tacgcggcac acatgcggat ggcggacttc
    13261 tggattgcga ccgtggcacg cgagtttgcc tcgggcaagg gcaaccccag tttacgtcgg
    13321 tagcgcctgg acagtttgga agcctctttg gagttccgga cgtcacatta cctgtccccg
    13381 ctgtgtcgtc cccactgtgt cccctgacgc ccgtcgtgat gtaacacgcg cttgtttgga
    13441 cgtcacggac acaccgcccg gtgtgttgaa aacgaatcga tcagtccgca tgagtatctc
    13501 gtcacccgca tcgaacagac aaaggtcatc gaaatgctgg attatgtcaa ccgcttgtac
    13561 gcggttggtg acagggcggg tcatgcgaag acaatgcgtt tctggtgaaa gttgggatgc
    13621 gttttttgga catgatttcc cgctgcgcgg gcgcataaac gacaagattg acatgatttt
    13681 tccgacaaag ggacaggtat ttagacactt atcagctttg ttccgaactg aatatcccga
    13741 cttcccggct tctacaggtt gtggggatga gtgacagtac cctgcggcgg cggcgagtat
    13801 ccggacggtt ggatcagaat gagtctgagc gggtctaccg gttgggcaag ttgtttcatc
    13861 tcgcgacacg ggtgttgggt tcgcgtgagc tggccgggca gtggttcaag atgcctgcaa
    13921 ctgcgctgag cgggtcgacg ccacttgatt acgcggacac cgagatcggc aaacgggaag
    13981 tcgagaagat gctttatcgt atcgactccc gactgttgaa gtgagaatat cttgggcgga
    14041 gatgatgaaa tgactttgca cccgtttagg gcggttttcg aagcccggcg ttgacggcgg
    14101 gggccgaaag acgtaggatg gcaaagtttt ctggtcggca atgttaggtt gttcggacgc
    14161 gagggtaacc tgcgagggga gtatggattt tctattggat gggatcaggg cgttcacttg
    14221 gcaacaggcg gtgatgtttg ccgtcggctt tacgctgatc tggctggcga tcaagaaaca
    14281 gtgcgaaccg atgctgcttt tgccgatggg cttcggcgct atcctggtga acatcccctt
    14341 tagcggagcg ctgaatcagg ttctgccggg ggtgggcgaa acccaggggg cgatccagtg
    14401 gcttttcgag gtcggcatcg aatcgtccga aatcctgccg ctcctgctgt ttatcggcat
    14461 cggcgcgatg atcgatttct cgccgctgtt ccagcgcccg atctatttcg ttttcgccgc
    14521 cgccgcccat ttcggcattt tcgcgaccat cgtcctggcc tgtgtgctgg gctttgattt
    14581 aagggacgcg gcggcgatcg gcatcatcgg cgctgccgac ggccctacca cgattctggt
    14641 cgcccaggtc ttgcagtcca agtacatggg tgcgatcgca gttaccgcgt actcctatat
    14701 ggcgctggtg ccgatcatcc aaccggcggt catttacctt ctgacctcgc gcgatgagcg
    14761 gcgcatccgc atggagccga caaaggattc cgacatcccg cgaactgtgc gcatcctttt
    14821 ccccatcgct gtgaccatcg tcgtcggtct gatctccccc gccgccgtct ctctgatcgg
    14881 ttttcttatg ttcggtaacc tgatccgcga atgcggcgtg ctggaatcgt tgtccagaac
    14941 cgcgcaaaac gagctgacca atctgatcac gctgttgctg ggcatcatca tcgcgttcac
    15001 catgaaggcg gaccaattcg tcacgcggga caccatcatg attctcgggc tggggctttt
    15061 cgccttcgtt tgcgacaccg ccgcaggcct gctgttcgcg aagtttctga acctcttttt
    15121 acgcaagaag atcaacccca tgatcggggc cacgggcatc tctgccttcc ccatgtcggc
    15181 gcgggtcatt cataaactgg cgatgaaaga ggacccgggc aatatcctga tcatgcaggc
    15241 cgtcagcgcc aacgtcgcgg ggcagatcgc ctcggtggcc gcgagcggcc tggtgttgag
    15301 cctagtcctg aaatacctgc aatagcaaga gccggaggat cacccatgga acacgccgaa
    15361 aacgtacgca tctcacttac tatcatgtgg caaggcatgc tggggttgtt catcattacc
    15421 attgtcatgg cttggctgac ctccaccatc attaagtttt gcaaacgccc cgaaaataaa
    15481 gaaactcctt cggactgagc ccctttcgta gtcccgcagc cccttccgcc ccgactgtgg
    15541 caaacgggag cgattcaata aagttgaatc gcagaaggtt aaagagttca gagggcaggg
    15601 gttttgcggc aaggccaaga agtccgccaa aacacgcgct cgacgcatat gccccctgtc
    15661 acttgcctat aaattgacag gattacagga ttaacatgat ttttttaatc ctgtcaccct
    15721 gtcataaaaa cgtatcccag agttttctcg cggctccggc gcgcggcgag cttgcctata
    15781 acttacaccc aaagttatcc tcatttgcat tcccgtgcga agcgcgggtg gcgggctaac
    15841 cgattgcaag ttgttttgta ttcgccattc attcgggggt cgaaagggtg taggatggtt
    15901 gcgttctcta cccaccacag gagcaccgca tgaaaatcag cttccctagc aaaggaatca
    15961 cggagcgcac gtcgcgtacc cgtacgggcg gacggatctc cgcaatcatc ttcgggctga
    16021 tcttcggcac cgtcggcctt gtgatgctgg tgctggctgt ccgcagttcc ctgcggcaac
    16081 tggatacgcg ccactggata gcgacaccct gcgagatcgt cgaatcgagc atcactcgcg
    16141 atgacgaaca gcatcgtttc gacaccgtgt tcacctacag tttcaacggg cacagctaca
    16201 ccagccgccg cttcacccac gagggcggtc tgcgggagaa acatatcaac gatgcgcagc
    16261 ggctgctcag ccagtacccg caaggcacca gcacaacctg ctacgtcaat cccgcagcgc
    16321 cggacgatgc gctgatccgg cgcgatcttt ccgtgggcgg catgatcgtg ccgatcctct
    16381 tggcgctgcc gcttgtcctc tccggctacg gccttatctt ctttgtctgg caccgcaacc
    16441 gaccggaacc gccgacaagc gacttttccg gcggcaagcc gtcacgcggt accatggccg
    16501 tgtccatacg ctttggaaca atttttatcg tggccggtct gggcgtgacc tattttacct
    16561 tccttggccc atttctccgg cagtgcgccg cgcgcgactg gacaccggtc gaggcggtcg
    16621 tcgagcaaag ccgggtgcgc tcgcattcta gcgacgaaga cacaacctac tacagtgtgg
    16681 acatcaccta ctgttatcgc atcgacgggc gtgactaccg gggcgatcga tacaacttta
    16741 acaccggttc gagcagcgac tacgagggca agcagcgggt ggtcgccgcc catccgccgg
    16801 gaagtacgct ctttgtctat gtcaatcccg ccgatccatt cgacagcgtg atcgtgcgcg
    16861 acatgggcgc aaccctctac ctcggcctgc tgccgctgat ttttaccgtc atcggggcta
    16921 tggcgctgat ctccggcatc ctccgtcagc gcaggcggct cgcccacccg ttagccccac
    16981 cctccgcctg gaccctgtgc ccggcgttcc gtgccgggcg gccgctgtgg gtgcttgcct
    17041 tcgcgctctt ctggaacggc gctacctcgg tctttgtcgt gcaatgtctc cgtgaatggc
    17101 aaaagggcgg acggcctatc ttcttgacgt tctttttggt gcccttcgtc ctgatcggtg
    17161 ccggctgcgt cgtcggattt ttcgtcgagc tgctccgcct tttcaacccg cggatcatcc
    17221 tgatcccgcc accggatccg ctggtgccgg gtgcgcccac gatgatcgcg tatcgcattc
    17281 gcggacggat agaccgagtg acgcggctga ccatatccct ggtgggaagc ggcgtcgcca
    17341 aggaacatgg cgacgataca gcggccagcc gcgaacttca ccggctcgtc gtctacgagc
    17401 aggagcagcc catgctgatg caggagggct tgttccggct cacgttgccg ccggagctca
    17461 tggggcgggg tgcggatccg cacaacccga ttacctggca cctcgaggtc aagggggaga
    17521 taccacgcgg gctcgatatc gtggaaacct accgcctgat cagctagcga cagaggatgt
    17581 agaaaatctg gagtgcggtg acaagagccg cgctgtgcac gggtcgcggc accgcttttg
    17641 cgcaccgctt tgccgccaca ctcgtgctca aaaagcggca tcgcgcggaa taccgcttgc
    17701 cgccgcactc caattttttc gcctgacttg cggagcatcg gtatcggaat tgggatcgct
    17761 atcggaatcg gcgtcacatt cgtatttcgt gaatgagaaa acccaaaagg aaaaatctgc
    17821 ggatgaaact ccgaaacaaa tccctgcgtt gcaaccactc gaaatgctcg gtataatctg
    17881 ggtaaaatga aggcgaagga tatacagaag gccggtgttt cgtcggggcc gttgatgaaa
    17941 ctggctttga gtttggtggg gtcggcaacg aaggc
//
LOCUS       DAOSLE010000050        17810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3385_length_17810_cov_7.895184, whole genome shotgun sequence.
ACCESSION   DAOSLE010000050 DAOSLE010000000
VERSION     DAOSLE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17810
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3385_length_17810_cov_7.895184"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..508
                     /locus_tag="PK576_05090"
     CDS             143..508
                     /locus_tag="PK576_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE61001.1"
                     /translation="MFSDRLRTLRGELKQAEFARKVGIAQNTYSRYESGERVPDIEVL
                     ARLAARLGVSADWLLGLDSEDAISTTAALPDQNAKPCEPVAAAYCPACGQKDVTIASQ
                     AESIVNLTRSLAALANLRA"
     gene            777..1334
                     /locus_tag="PK576_05095"
     CDS             777..1334
                     /locus_tag="PK576_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE61002.1"
                     /translation="MHRFPVPPRFRDKSPNGIALNHRIMVEDMPAWSPCLRSKTTIRT
                     SDTRYFTDPSIAAAAMGLGPGDLINDIQSLGFFFEALAVRDLRVYAEALGGAVSHFLD
                     RNGLECDAVVHLRNGSSGLVEVKLGGEALIEEGASTLNALSGLLDTTRMKAAAFKMVL
                     VAVGKYAYRRPDDNVIVCPIGCLKP"
     gene            1514..2239
                     /locus_tag="PK576_05100"
     CDS             1514..2239
                     /locus_tag="PK576_05100"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015711694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOE61003.1"
                     /translation="MKKLIEKLTKARCVAVFTGAGVSTLCGIPDFRGPQGLYKQPNAE
                     RIFDIEWFRRDPSVYYKGCMELVYGLRRFKPGPVHHALVHLERSGIVRGIITQNIDML
                     HQKAGSKTVYEVHGSPILHHCFGCGQEKTFDEICAMLEDTPVPRCGHCGEAFKPDITF
                     FGEMLPEQAFEQSVALARQADLMLVLGSSLTVHPVASIPPLTVRAGGELVIVNAQPTP
                     LDQIATLLYPDLNAFADAVLQVH"
     gene            2750..4507
                     /locus_tag="PK576_05105"
     CDS             2750..4507
                     /locus_tag="PK576_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE61004.1"
                     /translation="MKKSRRIGLLVLTLAGASLRAGERMPLWPEGKIPDFQDHQVGVM
                     TDAMWLPGTWGKPNPAFKPEEHRMPYIEWYGKAENPNGACIILISGGSYQNCCDVNLV
                     KKMWPNRLIELGYQCVNFVYRTPRPKGLPIYQSAWQDGQRAVRLVRSEAAKRGFDPER
                     IGLISMSAGGHLGVMMATSSLTPAYEKVDELDDLPCHVNAAICNAPAYATGLGAAGGA
                     APQDGTLVPYPTINPCFKFDAKTCPISFHHGAVDPYSPNGSTLCYREVRKRSVPSELH
                     LYADLGHGAHGLERAVEFLRQLNFDGRLGAEEERRIFTGTHTAEQVCEKLWPEGRVPS
                     HSAAQTNTATLTWFVPKTLKTKAIQVIFPGGAYTHCNVRGEGRPVAEYFNAQGMTAVV
                     VEYRCPRPKNLPKHWTAWQDAQRAIRLVRAGAVARGLDPERIGAMGFSAGGHLTLMTA
                     LSSTYPAYNPVDDVDKKVSCRVAWACPIYPAYVLSDGADGAHARGGNDDKATLVPELL
                     FDVNTPPMCFVHGDADSITAMGSVKVWEKLRRMGASSDLHTLATRGHCFQFKAAPGTG
                     SWTWLDQVWDFLVRKGFVK"
     gene            4532..6823
                     /locus_tag="PK576_05110"
     CDS             4532..6823
                     /locus_tag="PK576_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61005.1"
                     /translation="MMKRVFSLALMSAAAPLFAATYYASPIGTSSAAGTEADPYDIAT
                     AIAKLGNNTYDDVYLLPGTYKVGSASLAAIDYYARLIGKGATRDDVVLDFEGAGTALK
                     VQTGASVCNLTISNFVNCSAMEIINYNGYGELIVSNCVIRSGSGTKPGMTMTGAGPIL
                     VVDSVIRDIDGNISGGGYGVGVNCSAAGACFRRCTFANNTYTGWVGLGGAAGYFSAAA
                     LVENCIVSNNSATGYGGGFHTTTGMRIKDTLFIGNSSSANGGGAIYSQSGANNTNFIV
                     GCTFIDNRTTVSSVKGGAIFAGFYNTLVSNCSFTANASLSNGGAIHCYDSATNKTIGI
                     EIVGCGFTNNTASSGGSAITIGRRGAERIEPPFNIRNCHFKGNTNSANATGGGIQLYG
                     FVQANVEDCDFEDNQAGVSSGEGGAIGRTNDAWNEAMARAVCRRCRFVNNRAAVGAAI
                     AATASLYDCDFFGNIATNSNASGCNAIVYLYGKRLVAKEYLDEAFTNFVSGCHFEGNV
                     VYGTTMGIMAPFGDFRCYDTTFSNNVGYTSHITGGWSVNLRAPTYFEVDRCQFVGNTA
                     ASSGSGFHCEKGTMNDSMTNGWVRNSLFHKNYSVTSGGAACGMIQLGWTNTWISGCTI
                     VSNYIYGGIWAAVNTSCTQSNCVANCIMYMNDTNSGTQYRDVNGSADKQPYFHNCFIS
                     NGKFTDNGCLGKDQDPGFVDIASGNLQLRRDSVCVDKGDNEPWMFAGKIPDLAGRPRI
                     SGSSVDIGCYEYCPILRFILSIR"
     gene            6912..11546
                     /locus_tag="PK576_05115"
     CDS             6912..11546
                     /locus_tag="PK576_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE61006.1"
                     /translation="MKMCVYAAAVLAAGAVFAAEQKTGEPEAKISIYADRPEFLYGAD
                     EKVEFVVKATSADGAPFTNGVLEVILDNYGGRGLFREKITDEVKLAKPVTVRADPLGK
                     PGFMRCRVLYRSPSGKETWEYAAVAVDPRAITSGAVRPKDFDAFWDGEIVRLEKEVPD
                     GVTAVKDEKASTETHTCFRMCAKVFGERSVWGFLTVPNGAFAAEPKRYPVKINVPGAG
                     VGVYWFPAQKQRGEAITLTINVFDFEPGWDTGRDWKIHYERYHDMHRRWEQKGGLREK
                     SQSWAAGFGQSRTNTFYYGAILGANRMVNWLCSQPYIDKKDVAYRGQSQGGAFGIFLA
                     SLNTNLTAATISEPALTGLTAEDEDKHQPGWPNPINMQTNDAAKKIAREVAPYFDGAH
                     FAPRIKCPVRFAVGYIDLLCPPHCVYAAYNALKPTVDRRIVLGPGVGHGCPKEPYQDM
                     EQRLTDSWKKAAPYAWAEEFFNPDHPGLTDRYPYPDRTAPSTYLYVAPDGDDGAEGTL
                     EKPLRTMEGARDRLRKLTLSGRRYLPRGGGVVFFRSGIYVITNTIKFTKNDSAHFKWS
                     PIVYRAYPGEKPVFTGGLEFSGTAELKDPEKLSQLPEGSKGKVKVIDLKAKGFDDFAP
                     QAAFGYRPKKPCHTVTDLYRDGKPLDVAVSPNPDIRLEDYYRIGEVLDKKHDVFKADF
                     PDMDKWAKEEDVMAQGWWTFLWASTTIPVEKIDPVAKTVTVAREPNIFYYAATSNNVF
                     TLENGLPALDRENEWVFDRKNGLAYVLEPKGAKAPVYTVTRLAKPMITVTDVNNFRIE
                     GLTFEYGRTDAIIVSNAVNFAFLGNTVRNFGGRGLQVYEAKEARVQNNTFHHFGFAAL
                     ILTGGDRPTLTPGMCIIENNDISFTGRRQRTSPESSTCQVGGCGNVLTHNWFHDTPGS
                     ALRLAGNDHRVSFNRFERCSFMSDDNGAIDMCNNMSYAGNRFVFNEFYDIGYKNPRCG
                     NAGIRLDDFISQQYLYGNRFVNASGGKFGAIQLNGGRDNISENNWIEGSQWAFSVDGA
                     VGAWWNRNQTMDYNVKKYSGDVSVYDEPYISRFPRTPEIIREAHSCTNTFRRNVIIGA
                     AALKEKRTWHQTVFEDNWMFDKIPTGEELAKTKFKPIPPAGSAGIYGIDKEPEIARPP
                     MIEQKFRDGIGRTFRKLQKGGDVRIGFFGGSITAMYGWRNFILKKLAEDFPQANIIEV
                     NASLGGAGSLSGAFRMRHAILGKKCDLMIIEYATNDTENSPAMNLRSMEGCVRQAWNS
                     DPEMDLLFVYTMNMSQFETYKLGLTSDTAYIHDLIADHYGIPSVNFCPRLMEEINSGR
                     WVYMAADIRNKFGIKPGEPDYEKRFEEAKKKDGRTIFTFDNCHPSADGHKLYHEILAK
                     AFDDMRARNDPPANHAAKMVLPPLAADNMEKAKLVFPDETIFSKGDWSILPKDHKESK
                     KFQRETDRIWFTDKIGAKIRFRFRGRAVQMYNIRGPKSCAIKVSVDGNPPFTRVLADP
                     WSAGSTLIWSEKIFNGCDSIHTVEIEIMRNPKHGGTELELGGLQLDGELL"
     gene            11621..13993
                     /locus_tag="PK576_05120"
     CDS             11621..13993
                     /locus_tag="PK576_05120"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOE61007.1"
                     /translation="MKKRVLICVAAAAMAAHAATIIEAGKSGYAIVTGAEAVEIAAAD
                     ELREIIMRSTGVELPVVCTTNLNKQALLQKADHFLFVGRSPEAETVFGADDLGPNDYA
                     VTEKKPFLGLFGGGDIWLRGNNWNGTLLAVYAFCEDVLGFRHFMPEEGGEKIVKTDHV
                     KTGGYGFRRTEFFPYGRGLTHIYLYGTKDLNAFMFRNGSFVPQRWVKDTKFRDIARPN
                     ACKDSGHGFFLYMPCKRYGRQFYEWDEDIDFFAIHPEWFSMNAVGQRNDRMQLCFSNK
                     GLRKAFTKRVLERCRRVGGEGELTVGANDVPGAFCHCPECKALQKKYEMPAGAYYDYL
                     PELCAAVAKEYPRITLSTLAYRKQQTENFPKGIDTFPDNFVCDFAPVDDNQSFNIGGK
                     GNEKTLENLKKWCAACKTVTYWYYACTKHPFGPVSRVAGDLKAMHEAGTDGAKLCGLY
                     APSLGCMLDYLFLRLTLDPYQDPWVRVKEFCDFAYGGASDDVMTLIRDWDQLWFEPNP
                     FVSIDAGISSMKIYRGEDIVRWIKILDAAEKKLGGDEYVKRNFSWVRWDVDLLLLTFW
                     PQVRAVKNLPPEINPETAYARMQTVTLARRYGRAPKKGEEPKGPYADAKSAYLITKAF
                     GKPIPAPLCDLPADQVIQLPQCGGFHGMTDPDAACGAAKSEVFPTNSTVFTTHKVGFD
                     YYDTNRKRMIRHGELDFTGFTPGEYKLYFISKVKIVPGALITFASWWGIGQNLTNYYP
                     EGDPDREFEIWASLKFIGPKFGTETKDGKNRMVCDRIFLVDKGGKKYKGE"
     gene            complement(14201..17740)
                     /locus_tag="PK576_05125"
     CDS             complement(14201..17740)
                     /locus_tag="PK576_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61008.1"
                     /translation="MGAAKKMKWISTFLNFATILALATSCVNRPDTLPASKIAVPQAP
                     FAADAAELYQPEAVMFSDKIVSGSKALIWNSGVRGGLSPLQLLIDEQEVLKTRMHASP
                     EAGWNPFTTFDTTYDPQKGIERSCPFKTTPSAGGTFRQRIFLRPDGKAEFTLRYEMED
                     YATLKGPTLVFSIPKPTCFGRQLAVCGKDGQRVYTFPDTNTKWTLGAKSVWINCFHVA
                     KPSRIEFDPEHPAKAFALEFPPDTCESLTLFRSDGTAELYFSWNPRCRTEEFSVILDF
                     GRSARTAKPQCVVNGINFTLNNDYDVPCYDERGNLLVNPSFESGPRLYHNVPSGMDLY
                     PHITEVDARSGRFALACGGAKTFNFPTLAGRDYTFSCYVRATVTNRCIGSIHCDTYLW
                     NNPTKPKWFGYSQEWKRYAYTVTNWPFRAIAFQINADPGVLIDDVQFEEGAEATAYKG
                     NPIGIELKTDAPDSFYAPPGTAFRARFVVRGPNSCKGSIAITVHDFFGRDVWKGEQAF
                     DLAEGEMTLPPLPESAFEAKGVYAIRMRVAGEGFKPYFDCVRLARFTYADGTAPNRRL
                     HQTTTVTWPTTAQTNEMERIRHLYQVLGLDPHAYALGGHGRDFDPELGRAIAARSEQY
                     GFHVEGHVFSTRVMHDTGFTTAEKYSDDLIKKVADICEEYARTRPYIDTWYGPGEASG
                     WVKTCQNRDWKEFAKLMRAAHEAIHRGNPKARFSAYGTCNLGEQGRREILEFLETAKA
                     LWPDFKFDTVDVHPYRPHPEFPDYDRDWKALFDGLDALGYSDMIVEATEGGYFVPIRC
                     VPWAGISPWQDTLSKDSYCSQHIPSMDIGWGERAAAAMLLRYNLVSYKYGARIGRATS
                     WGLKHVDSRNPFAQYVATAAQTELLGKAVFLEDIRFAPGSRAYVFDDREGHAVAAFWR
                     FTLALDYGTENANTMTLNLAGLDPEIFDMMGNRVQYETIRDQAGQRARLPLGNFPVYM
                     RVAKRDAEKLALAIRTAETDIPSQAEIQLREVKVIQARHTSGEPEWDNIEAVPLNGGK
                     ARFAWNEETLYLRWEAHTIVVPVFCFDTLADERENARIGFEGADENDLVYVVRIKDAR
                     NSDHADGWEVFRREAPDLQLTGGVDRSLLPQVVEPAVRLRVEKKPQQPRTFTLAFPRR
                     QLEPMRLTAKSRFGFAQNEMGFCDGYIEVLLVK"
BASE COUNT         3638 a         5098 c         5455 g         3619 t
ORIGIN      
        1 ttcccgtccc tttttgttgc ataaggccac acgttggcct aaaataaaac cgagacttct
       61 tcgattgaac ggcataatgt tatcccattt gggaaagacg gtcaatgcca aatgggaagt
      121 ttttcagggg gcttttttga gcgtgttttc cgacagattg aggacgttgc ggggcgagtt
      181 gaaacaggcg gagttcgcgc gcaaggtcgg tatcgcccag aacacgtaca gccgctacga
      241 gtcgggcgag cgcgtcccgg atatcgaggt gcttgcccgg ctggcggcac gtctgggcgt
      301 ctccgccgac tggctgctcg gcctcgacag tgaagacgcg atttcgacaa cggccgccct
      361 cccggatcaa aacgccaagc cctgtgaacc cgtcgccgcc gcctattgcc ccgcctgcgg
      421 acagaaggac gtgaccatcg ccagccaggc cgaatccatc gtgaacctca cgcggtcact
      481 cgcggcgctc gccaatctcc gcgcatagcg cggagattgg agttaggaat taggagttag
      541 cctgccgcgc ggcttcgccg cgaaaaaacc ttggaatacg tttaggacat gatttccgac
      601 agcttcgctt cggcggagac aatcctgaaa atcctgtaat cctgtcaatt tataggcaag
      661 gaatttttgg gaaccgccaa ggtttttggg gggcgtggct ccagccgcag gtcgctggtc
      721 gccggtcaac ctcttattgc gaccacgcag tggctcgcaa ttttgagagt aaaggcatgc
      781 accgctttcc ggttccccca cgcttccgcg acaaatcgcc gaacggaatc gcgctaaatc
      841 accgaattat ggtcgaagac atgccggcgt ggtcgccttg tctgcggtca aagacaacga
      901 tccgcacttc ggacacgcgc tatttcaccg atccgtccat cgccgccgcc gccatgggat
      961 tgggacccgg cgacttgata aacgacatcc agtcactcgg ttttttcttc gaggcactcg
     1021 ccgtgcgcga tttgcgcgtg tacgctgaag cgctcggagg tgcggtcagc cactttctcg
     1081 accgaaacgg gctggagtgc gatgccgtgg tgcatttgcg gaacggctcc tccggtcttg
     1141 tcgaagtgaa acttggcggc gaggctctca tcgaggaagg cgcttcgaca ctgaatgcgc
     1201 tttcgggact gctcgacacg acacggatga aggcggcggc attcaaaatg gttttggtgg
     1261 ccgtaggcaa gtacgcatat cggagacccg atgacaacgt gatcgtctgt ccgatcggat
     1321 gcctcaagcc ctgaccccgg caccgcctat tgaggtgatt gcgacaaggt gctctggtta
     1381 taggcaaggt aaagggaaac agccttgccg caacacataa atctgactat gctctattct
     1441 agcgccttac cggccgataa ggccaataaa aggttgtttc tcacacgaag ggcgcgaagg
     1501 ggaaagggtt gtcatgaaaa aactgatcga gaaactgacg aaggcacggt gcgtggcggt
     1561 tttcaccggc gcgggcgtct caacgctttg cgggattccg gattttcgcg gaccgcaggg
     1621 actctataaa cagccgaatg ccgaacgtat cttcgatatc gaatggttcc ggcgcgatcc
     1681 ctcagtttac tacaagggct gcatggaact ggtgtacggg ctccggcgtt tcaagcccgg
     1741 cccggtgcat cacgcgctgg tgcatctgga gcgctcggga atcgtccggg gtatcattac
     1801 gcaaaacatc gacatgctgc atcagaaggc cgggtcgaag accgtgtacg aggtccatgg
     1861 ctctcccatc ctgcaccact gcttcgggtg cgggcaggag aagacctttg acgagatctg
     1921 cgcgatgctg gaggacacgc ctgtcccgcg gtgcgggcat tgcggagaag ccttcaagcc
     1981 tgacatcacg ttcttcggcg agatgctgcc tgagcaggcg tttgaacaat cggtcgcgct
     2041 ggcgcggcag gccgacctga tgcttgttct cggctcgtcg ctgaccgtcc acccggtggc
     2101 ttcgatcccg ccgctgactg tccgggcggg aggcgaactg gtgatcgtca atgcccagcc
     2161 gacccctctg gatcaaatcg caacgctgct ctatcctgac ctgaacgctt tcgccgatgc
     2221 tgtgttgcaa gttcattagt tcgttggtgc gttacggctt aacaaggttt gtacagtctt
     2281 ttgattgcgg gctccgcccg cgttgggggt taaggctgtg aggggttaag gctgcaaggt
     2341 gttaaggctg tgaggggtta aggctgtgag ggaatatgcg gcgggtcgca tcaaacagcc
     2401 tcacagtctc acagcctcaa ctcctcactg tctttgagat tgcgggctcc gcccgcgtta
     2461 gtgcggaatc gggatcggaa tcgctatcgg gatcgtccgc ggacgcctcg gggatgcgtc
     2521 cctgcccctg gcgcgtagcc gaggacgtcc gaggaggcgg aacgctgaac gtgaaaccag
     2581 aaaccagaaa tctcggcact cttggcgatt ccagaaaccc taaagcgttg agttttactg
     2641 tttataggtt tagtggttga ctggtttcat ctccctcgta caatgggtgg ctatcactgg
     2701 aggcggcgtg ttcccggtct gcccctggag gacaatgacg gagtcgatca tgaagaaatc
     2761 tcgtcggatt gggttgctgg tgttgacgtt ggcgggtgcg tccctgcgtg cgggcgagcg
     2821 gatgccgcta tggccggaag ggaagattcc cgatttccaa gatcatcagg tcggggtgat
     2881 gacggatgcc atgtggcttc cggggacgtg ggggaaacct aacccggcgt tcaagccgga
     2941 ggagcatcgt atgccgtaca tcgaatggta cggcaaggca gagaacccca atggggcgtg
     3001 catcatcctc atttcaggag ggagttacca aaactgctgc gatgtgaatc tggtgaaaaa
     3061 gatgtggccg aaccgtctca tcgaactcgg ctatcagtgt gtcaatttcg tctatcggac
     3121 gccgcgtccg aagggactgc cgatctacca gagcgcctgg caggacggac agcgcgccgt
     3181 gcgtcttgtc cgttccgagg ccgcaaagcg cggtttcgat cccgaacgca tcggcctgat
     3241 ctcgatgtcc gcaggcgggc atctcggtgt gatgatggcg acgagttccc tgacgcccgc
     3301 gtacgagaag gtggacgaat tggacgatct gccctgtcat gtcaatgccg cgatctgcaa
     3361 tgccccggcg tatgcgaccg gacttggcgc ggcgggcggt gcggcgccgc aggatggcac
     3421 gctcgtgccg tatccgacga tcaatccgtg tttcaagttc gacgcgaaga catgtccgat
     3481 cagcttccac catggcgcgg tcgacccgta ttcgccgaac ggctcgacgc tttgctaccg
     3541 cgaagtgcgt aagcggagcg tcccgtctga actgcacctt tacgcggacc tcggccatgg
     3601 ggcgcacgga cttgagcgcg cggtcgagtt tctccgccag ctcaatttcg acggacgcct
     3661 tggcgcggag gaggaacggc gcatcttcac gggtacgcac acggccgagc aggtttgcga
     3721 gaagctgtgg cccgagggac gcgtcccgtc ccattcggcc gcccagacca atacggcgac
     3781 cctcacttgg ttcgttccga aaaccttgaa gacgaaggcc atccaggtga tttttccggg
     3841 gggcgcctac acgcactgta acgtccgggg ggagggacgt cccgttgccg aatacttcaa
     3901 tgcgcagggg atgacggcgg tggttgtcga gtaccgctgt ccgcgaccga agaatctgcc
     3961 gaagcactgg acggcttggc aggatgcgca gcgggcgatc cgccttgtcc gggcgggagc
     4021 cgtggcccgc ggactcgacc cggagcgcat cggcgcgatg ggcttttcgg cgggcggaca
     4081 cctgacgctc atgacggcgc tcagttcaac gtaccctgcg tacaatccag tggatgatgt
     4141 cgataaaaag gtttcctgca gggtcgcttg ggcatgtccg atctatcccg cgtacgtgct
     4201 ttcggatggc gctgacggtg cccatgcgcg aggcgggaat gacgataagg cgacactcgt
     4261 ccccgagctt cttttcgacg tcaatacgcc gccgatgtgc tttgtgcatg gcgatgccga
     4321 ctccattacg gcgatgggat ccgtcaaggt ctgggagaaa ctccgccgga tgggcgcgtc
     4381 gagtgatctt catacgctcg cgacccgtgg gcactgtttt cagtttaaag cggcacccgg
     4441 taccggcagc tggacctggc ttgatcaggt gtgggacttc cttgtccgca aggggttcgt
     4501 gaagtgaaat ccgaataaca ggagaatgga catgatgaaa cgtgtttttt cactggcact
     4561 gatgtccgcg gccgcgccgc ttttcgcggc gacttactac gcttcgccga tcgggacgtc
     4621 ctcggccgca ggtacagagg cggatccgta cgatatcgcg acggcgatcg ccaaactcgg
     4681 taacaacacc tatgatgatg tctacctcct accgggaacc tataaggtcg gcagcgcctc
     4741 gctcgcggcc atcgactact acgcgcggct gatcggcaag ggcgcgacac gcgatgacgt
     4801 ggtcttggac ttcgagggcg cgggtacggc tctcaaggtt cagaccggcg caagcgtctg
     4861 taatctcacg atctccaact tcgtcaactg ttcggccatg gagatcatca actataacgg
     4921 ctacggggaa ctgatcgttt ccaactgcgt gatccgctcc ggctcgggta ccaaacctgg
     4981 aatgacgatg accggagcag ggcctatcct tgtcgtcgac agcgtcatcc gtgacatcga
     5041 cgggaacatt tccggaggcg gttatggtgt gggagtcaat tgctcggctg ctggcgcatg
     5101 cttccggcgt tgcacgttcg ccaacaacac ctataccggg tgggtcggtc tgggcggtgc
     5161 cgcgggatat ttttccgctg cggcgctcgt tgagaattgc atcgtctcca acaacagtgc
     5221 aacgggctat ggcggaggat tccacaccac cacgggtatg cggatcaagg acacgctttt
     5281 catcggcaac tcttcgtctg cgaacggcgg cggcgccatc tacagccaga gcggcgcgaa
     5341 caacaccaac tttattgtcg gctgcacgtt tatcgacaat cgcacaaccg tgtccagcgt
     5401 gaagggcggg gcgattttcg cgggttttta caatacgctc gtctccaact gcagcttcac
     5461 ggcgaatgct tcgctcagta acggcggcgc cattcactgc tacgactccg ccacgaacaa
     5521 gacgatcggc atcgagatcg tcggctgtgg cttcacaaac aacaccgcgt cgtcgggcgg
     5581 cagcgcgatc acgatcggac gacgcggagc ggaacgcatt gagccgccct tcaacatccg
     5641 gaactgccac tttaagggca ataccaactc ggccaatgcg accggcggcg gtattcagct
     5701 ctacgggttt gtccaggcga atgtcgaaga ttgcgacttc gaggacaatc aggcgggggt
     5761 gtccagcggc gagggcggcg cgatcgggcg caccaatgac gcttggaacg aggcgatggc
     5821 ccgcgcagtc tgccggcgtt gccggttcgt gaacaaccgg gcggctgtgg gtgcggcgat
     5881 agccgccacg gcctcgctct atgactgtga cttcttcggc aacatcgcga ccaattccaa
     5941 cgccagcggc tgcaacgcga ttgtttatct ctacggcaaa cgcctggtcg ccaaggaata
     6001 tttagacgag gccttcacga atttcgtatc gggttgccat ttcgaaggaa acgtcgtcta
     6061 cggaaccacc atgggcatca tggcgccctt cggtgatttc cgctgttatg acacgacctt
     6121 ctcgaacaat gtcggctaca cgagccacat tacgggtggc tggtcggtca atttgcgcgc
     6181 gccgacctat ttcgaggtgg accgctgtca gtttgtcggg aacaccgctg cgtcgtccgg
     6241 atccggcttc cactgcgaga agggaacgat gaacgacagc atgacgaacg gttgggtccg
     6301 gaattcgctt ttccacaaga actattccgt cacttccggc ggcgcagcct gcggcatgat
     6361 ccagctcggt tggacgaaca cgtggatctc gggctgtacc attgtctcca actacatcta
     6421 cggcggcatc tgggctgcgg tcaacacgag ctgcacccaa tcaaactgcg tcgctaactg
     6481 catcatgtac atgaacgaca ccaacagcgg gacgcagtac cgggatgtga acggcagcgc
     6541 ggataagcag ccgtatttcc acaactgttt catcagcaac ggcaagttca cggataatgg
     6601 atgcctcggc aaagatcagg atcccggatt cgtcgacatt gccagtggca atctccaact
     6661 tcgcagggat tcggtgtgtg tggataaagg cgacaacgag ccttggatgt ttgcgggaaa
     6721 aatccccgat ctcgcaggac ggccccgtat aagtggttct tccgtcgaca tcggctgcta
     6781 cgaatactgc ccgatccttc gtttcatcct gagcatccgc taaacgtttc aaaccgtcta
     6841 gcccttccga gcctcatcaa catcatctcg cgaagatggg gggacggtcg gctaatggaa
     6901 aaggaaatcg aatgaagatg tgcgtttatg cggcagcggt gctggccgcg ggggcggtgt
     6961 ttgccgcaga gcagaagacc ggggaaccgg aggccaagat cagcatttac gcggatcgtc
     7021 cggagtttct ctacggcgcg gatgagaagg tcgagtttgt cgtgaaggct acgtctgcgg
     7081 acggagcgcc gttcacgaat ggcgtgctgg aagtcatcct cgacaactac ggagggcgcg
     7141 gtctttttcg cgagaagatc acggacgagg tgaagcttgc gaaacccgtg accgtaaggg
     7201 cggatcctct cggtaagccg ggtttcatgc gttgccgtgt gctctatcgg tcgccgtccg
     7261 gaaaggagac gtgggaatat gccgccgtcg cggtcgatcc gcgtgcgatc acgagcggtg
     7321 cggtacggcc gaaggacttc gacgcgttct gggatggcga aatcgtgcgg cttgagaagg
     7381 aggttcctga cggcgtcacg gccgtgaagg atgaaaaggc ctccacagaa acgcacacct
     7441 gctttcgaat gtgcgcgaag gtgttcggcg agcgcagcgt ttggggtttt ttgaccgtac
     7501 cgaacggcgc gtttgccgca gagccaaagc ggtacccggt gaagatcaac gtgccggggg
     7561 cgggtgtggg cgtctactgg ttccccgccc agaaacagcg aggcgaggcg atcacgctta
     7621 cgataaacgt cttcgacttc gagcctggct gggacacggg acgcgattgg aagatccact
     7681 acgagcgcta tcacgacatg caccgtcgct gggaacagaa aggcggactc cgcgagaagt
     7741 ctcagagttg ggcggcgggc ttcggccaga gccgcacgaa cacgttctac tacggcgcga
     7801 ttctcggcgc gaaccgtatg gtcaactggc tctgctcgca accgtacatc gacaaaaagg
     7861 atgtcgccta ccgcggacag agtcagggcg gcgcgttcgg tatcttcctc gcctcgctta
     7921 acacgaacct cacggcggca acgatctccg agccagcgct cacgggactc accgccgagg
     7981 acgaggacaa gcaccagccc ggatggccga atccgatcaa catgcagaca aacgacgccg
     8041 cgaaaaaaat cgcccgtgag gtcgcgccgt atttcgacgg cgcccacttc gcgccgcgca
     8101 tcaagtgtcc ggtacgcttc gcggtcggct acattgacct gctctgccca ccgcactgcg
     8161 tctacgcggc ctacaacgcg ctgaagccga ccgtcgaccg ccgcatcgtc cttggcccgg
     8221 gtgtcggaca cggctgtcca aaggaaccgt accaagatat ggaacagcgt ctcaccgaca
     8281 gttggaagaa ggctgctccc tatgcgtggg cggaggagtt tttcaacccc gatcatcctg
     8341 ggctcacgga tcgttatccg tatccggacc ggactgctcc cagcacttat ctctacgtcg
     8401 caccggacgg cgatgacggt gcggagggca cacttgagaa gcccctccgc acgatggaag
     8461 gagcgcgcga ccgcctgcgc aaactgacct tgagcggacg cagatacctt ccccgcggcg
     8521 gcggagtcgt ctttttccgc agcggcatct atgttatcac caacacgatc aaattcacca
     8581 aaaacgactc cgcgcacttc aagtggagtc cgatcgtcta tcgcgcgtat ccgggcgaga
     8641 agccggtttt caccggcggc ctggagtttt cggggacggc cgaactgaag gaccccgaga
     8701 aactctcgca gcttcctgag ggctcgaaag gcaaggtcaa ggtcattgat ctcaaggcaa
     8761 agggatttga cgacttcgcg ccgcaggcgg cgttcgggta tcgcccgaag aagccgtgcc
     8821 acacggtcac cgacctctac cgcgacggca agcccctcga tgtggcggtg tcgccgaacc
     8881 ccgacatccg tctcgaagat tattaccgca tcggcgaagt gctcgacaag aaacacgatg
     8941 tcttcaaggc ggactttcct gacatggaca agtgggcgaa ggaggaggac gtgatggcgc
     9001 agggttggtg gaccttcctc tgggcgtcga cgacgattcc ggtcgagaag atcgaccccg
     9061 ttgcgaagac ggtcaccgtc gcgcgcgagc cgaacatctt ctactacgcc gccacgtcca
     9121 acaacgtctt tacgcttgag aacggccttc ccgcgctcga ccgcgagaat gagtgggtgt
     9181 tcgaccgcaa gaacggtctc gcctacgtgc tcgaacccaa gggcgcaaag gcgccggtct
     9241 atacggtcac gcgtctcgcg aaaccgatga tcacggtgac ggatgtcaac aacttccgca
     9301 ttgagggcct gacctttgag tacggcagaa ccgacgcaat catcgtttcg aatgcggtga
     9361 acttcgcctt tctcggaaac accgtccgga acttcggcgg acgcggcctg caggtctacg
     9421 aggctaagga agcgcgtgtc cagaacaaca cgttccacca cttcggattt gccgcgctca
     9481 tcctgacggg cggcgaccgt ccgaccctga ccccgggcat gtgcatcatt gagaacaacg
     9541 acatctcttt tacgggacgc cgccagcgta cttcgcccga gtccagcacc tgccaagtcg
     9601 gcggctgcgg caatgtgctc acgcacaact ggtttcacga tacccccggt agcgcgctcc
     9661 gcctcgccgg caacgaccac cgcgtctcct ttaatcgttt cgagcgctgt tcgttcatga
     9721 gcgacgacaa cggggcgatc gacatgtgca acaacatgtc ctacgccggg aaccgctttg
     9781 tcttcaacga gttctatgat atcggctaca agaacccgcg ctgcggcaat gcgggcatcc
     9841 gtctggacga cttcatatcg cagcagtacc tgtacggcaa ccgcttcgtg aacgcttcgg
     9901 gtggcaagtt cggtgcgatc cagttgaatg ggggacgcga caacatatcc gagaacaact
     9961 ggatcgaggg ctcgcagtgg gcgttcagcg tagacggcgc tgtgggcgcg tggtggaacc
    10021 gcaaccagac gatggattac aacgtgaaga agtattccgg ggatgtgagc gtctacgacg
    10081 aaccctacat ctcgcgcttt ccgcgcacac cggagatcat ccgcgaggcc cattcgtgca
    10141 ccaacacctt ccgccgcaat gtcatcatcg gtgccgcggc gctcaaggaa aagcgtacgt
    10201 ggcatcagac ggtcttcgag gacaactgga tgttcgacaa gattccgact ggggaagaac
    10261 tcgcgaagac gaagtttaag cccattcctc ccgccggtag cgccggcatc tatggaatcg
    10321 acaaggagcc cgagatcgcg cgtccgccga tgatcgagca gaagttccgc gacggtattg
    10381 gccgtacgtt tcggaagttg cagaagggcg gtgatgtgag gatcggtttc ttcggcggtt
    10441 ccatcaccgc gatgtacggc tggcgcaact tcatcctcaa aaaacttgcg gaggactttc
    10501 cgcaggcgaa tattattgag gtgaacgcgt cgctcggcgg cgcgggttcg ctttcgggag
    10561 cgttccgtat gaggcacgcc atcctcggta agaagtgtga cctcatgatc atcgaatacg
    10621 cgacgaatga cacggagaac agcccggcga tgaacctccg ttcgatggag gggtgcgtta
    10681 gacaggcgtg gaattccgat cctgaaatgg atctgctctt cgtctacacg atgaacatgt
    10741 cccagttcga aacctacaag ctcgggttaa cctccgatac cgcctacatc cacgacctta
    10801 tcgccgacca ttacggcatt ccgagcgtga acttctgtcc gaggcttatg gaggagataa
    10861 actcagggcg ctgggtttac atggccgctg acatacgcaa caagttcgga atcaagcccg
    10921 gcgagccgga ttatgagaag aggttcgagg aggcgaagaa gaaagacgga cgcacgatct
    10981 tcaccttcga caactgccat ccgagcgcgg acggccataa gctctaccac gagatcctcg
    11041 cgaaggcgtt cgacgacatg agggcgagga acgatccgcc ggcgaaccac gccgcaaaga
    11101 tggtgcttcc gccgctcgcg gcggataaca tggagaaggc gaagcttgtc tttccggacg
    11161 agacgatctt ctcgaagggc gactggtcga ttctcccaaa ggatcacaaa gagtcgaaga
    11221 aattccagcg cgagacggac cgtatctggt tcacggacaa aatcggggcg aagatccggt
    11281 tccgtttcag gggccgtgct gtccagatgt acaacatccg cggaccgaag agctgcgcga
    11341 tcaaggtttc cgtggacggc aatccgccgt tcacccgtgt tctcgccgat ccgtggagcg
    11401 cgggttcgac gctgatctgg tccgagaaaa tcttcaacgg ttgcgattcg atccatacgg
    11461 ttgaaatcga aatcatgcgt aacccgaaac acggcggcac cgaacttgaa ctcggcggtc
    11521 tccaacttga cggtgagctg ctgtgataaa gatgccgtcg acattggctg ttacgagtac
    11581 gtgccggaaa acttcgggtt ctttatctct ctcaaatgat atgaaaaaaa gagtgctgat
    11641 ctgcgtcgcg gcggcggcga tggccgcaca tgcagcgacg atcattgaag cggggaaaag
    11701 cggttacgcg atcgtgactg gcgcggaagc ggtggaaatc gccgctgcgg acgaactccg
    11761 tgaaatcatc atgaggtcga cgggcgtcga gcttccggtg gtttgcacaa ccaatttgaa
    11821 caaacaggcc ttgctccaaa aggcggacca tttcctcttt gtcgggcgtt ccccagaagc
    11881 ggaaaccgtg ttcggtgcgg acgatctcgg tccgaatgac tatgcggtga ccgagaaaaa
    11941 gccgttcctt ggactctttg gcggcggcga tatctggctg cgcgggaaca actggaacgg
    12001 gacgctgctt gcggtctacg cgttctgcga ggatgtcctg ggtttccggc acttcatgcc
    12061 cgaggaaggc ggcgagaaga tcgtgaagac cgatcatgtc aagacgggcg gttatggttt
    12121 tcgccgcacc gagtttttcc cgtacggccg cggtcttacc cacatttacc tctacggcac
    12181 gaaggatctc aacgccttca tgttccgcaa cggttctttc gtgccgcaac gctgggtcaa
    12241 ggatacgaaa ttcagggata ttgcccgtcc gaacgcgtgt aaggattccg gccacgggtt
    12301 cttcctctac atgccgtgca agcggtatgg ccggcagttc tatgaatggg acgaggacat
    12361 cgacttcttt gccattcacc cggaatggtt ctcgatgaac gccgtcggtc agcgtaacga
    12421 ccggatgcag ttgtgcttct cgaataaggg actccgcaag gcgttcacga agcgcgtgct
    12481 ggagcgttgc cgccgtgtgg gcggcgaggg agaactcacg gtcggcgcga acgacgttcc
    12541 cggcgcgttc tgccactgcc ccgaatgcaa agcgttgcag aagaaatacg agatgcctgc
    12601 cggtgcttat tacgactatc tgccggaact ttgcgcggcg gtggcgaagg aatatcccag
    12661 gatcaccctc tcgacgctgg cttatcgcaa acaacagacg gagaatttcc cgaagggcat
    12721 cgatacattc cccgataact tcgtttgcga cttcgcgccg gtggatgaca atcaatcatt
    12781 caacatcggc ggcaaaggaa atgaaaagac gctggaaaat ctgaagaaat ggtgcgccgc
    12841 ctgcaagacc gtcacctact ggtattacgc atgcacgaag catccgttcg gtcccgtgag
    12901 ccgcgttgcc ggtgacctta aggcgatgca cgaggcggga acggatggcg caaaactctg
    12961 cggtctctac gcgccgtcac tcggctgcat gctcgactac ctgtttctgc gcctgaccct
    13021 cgatccatat caggatccgt gggtgcgggt gaaggagttc tgtgacttcg cttatggcgg
    13081 tgcctcggac gacgtgatga cgctcatccg cgactgggat cagctctggt tcgaaccgaa
    13141 ccccttcgtc agcatcgatg ccgggatatc ctcgatgaaa atctacaggg gtgaagacat
    13201 cgtccgctgg attaagatcc tcgacgcagc ggagaagaaa ctcggcggcg acgaatatgt
    13261 gaagcgcaac ttctcgtggg tgcgttggga cgtcgacctc ctgttgctca cgttctggcc
    13321 ccaggtgcgg gcggtgaaga acctgccgcc ggaaatcaac cccgaaaccg cctatgcgag
    13381 aatgcagact gtgacccttg cccgacgcta tggcagggca ccgaagaaag gcgaagagcc
    13441 gaaagggcct tatgccgacg cgaagagcgc atacctcatc acgaaggcat tcggaaagcc
    13501 tattcccgcg ccgctttgcg accttccggc ggatcaggtg atccaactcc cgcagtgcgg
    13561 cggtttccac ggcatgaccg atcctgacgc cgcgtgcggc gcggcgaaga gcgaggtgtt
    13621 cccgacgaac agtactgtct tcaccacaca caaggttggc ttcgactact acgacaccaa
    13681 ccgcaagcgc atgattcgcc acggggaact cgatttcacg gggttcacgc ccggtgaata
    13741 caagctgtac ttcatcagca aggtgaaaat cgtgcccggt gcgctcatca ctttcgcaag
    13801 ctggtgggga atcggccaga atctcaccaa ctactatccg gagggcgacc ctgatcgcga
    13861 gttcgagatc tgggcttcgc tcaagttcat cgggcctaaa ttcggcacgg aaacaaagga
    13921 cggcaagaac cgcatggttt gcgaccgcat tttccttgtc gacaagggcg gcaaaaagta
    13981 caagggcgag tgatatgaaa accggcccga tttttttgcg atgcgccttg atcggcctaa
    14041 cggcaggggt ttgctttgcg acgtgtggtt cgtccggact gttgcgcgac ggttgttggc
    14101 gatcccgttg aactttcagc gctctttggt ttcaggtttc gggaaccgcc ttcacgtgcg
    14161 gtctctgcct gtacggcggg acgtccaccc cttgtttgcg tcacttcacg agtaggacct
    14221 cgatgtatcc gtcgcagaag cccatctcgt tttgtgcgaa accgaaccgg ctctttgcgg
    14281 tgagacgcat cggttccaat tggcggcgtg ggaacgcaag cgtgaaggtg cgcggttgct
    14341 gcggcttttt ctcgactctc aatcgtaccg ctggttcgac gacctgcggg agaagtgagc
    14401 ggtcgacacc gcccgtgagt tgaaggtcgg gtgcctcacg gcgaaagacc tcccagccgt
    14461 cggcatggtc gctgttccgg gcgtctttga tgcgaaccac atagacgagg tcattttcgt
    14521 cggccccctc gaaaccgatc cgtgcgtttt ccctttcgtc tgcgagcgtg tcgaaacaga
    14581 aaacgggcac cacgatcgtg tgggcttccc accggagata tagcgtctcc tcgttccacg
    14641 cgaaccgggc cttgccaccg ttgagtggca cggcctcgat gttgtcccac tccggctcgc
    14701 cggatgtgtg ccgtgcttgg atgaccttga cctcgcgcaa ctggatttcc gcctgcgagg
    14761 ggatgtctgt ctcggcggtc cggatggcca gtgccagttt ctcagcgtcg cgtttggcca
    14821 cgcgcatata gaccgggaag ttgccgagcg gtaaacgtgc gcgttgcccc gcttgatcgc
    14881 gaatggtttc gtactgcacg cgattgccca tcatatcgaa gatctcggga tcgagcccgg
    14941 cgaggttcag ggtcatcgtg ttcgcgttct ctgtgccgta gtccagagcc agagtgaaac
    15001 gccagaacgc cgcgacggcg tgtccctccc ggtcgtcaaa gacgtaggcg cgtgagcccg
    15061 gtgcgaaacg gatatcctca agaaacaccg ccttgccgag aagctcggtc tgcgcggcgg
    15121 tcgccacgta ttgcgcgaac gggttacggc tgtcgacgtg cttgaggccc caactcgtcg
    15181 cgcgtccgat gcgcgcgccg tacttgtacg agaccaggtt gtaccggaga agcatcgctg
    15241 ccgccgcgcg ctcgccccag ccgatatcca tcgaggggat atgctgggaa caataggagt
    15301 ctttcgagag cgtgtcctgc cagggcgaga ttcctgccca cggaacgcaa cggatcggga
    15361 caaagtatcc gccttccgtg gcctcgacga tcatgtcgga gtatccgagc gcatcgagtc
    15421 cgtcgaacag cgccttccaa tcccggtcat aatccgggaa ctcgggatgc ggacggtacg
    15481 ggtgcacgtc aaccgtgtca aatttgaagt ctggccacaa ggccttcgcg gtttcgagga
    15541 attcaagaat ctccctgcgc ccctgctcgc cgaggttgca ggttccgtat gccgagaacc
    15601 gcgccttcgg attaccgcgg tgaatcgcct cgtgggcggc gcgcatgagt ttggcgaact
    15661 ctttccaatc gcggttctgg caggtcttga cccatccgga ggcctcgccg ggtccgtacc
    15721 acgtgtcgat gtacggccgc gttcgcgcgt attcctcgca gatgtcggcc acctttttga
    15781 tcaggtcatc ggagtatttc tccgcggtcg tgaacccggt gtcgtgcatc acccgggtag
    15841 agaacacatg cccttcgacg tggaacccgt actgttcgct ccgggcggcg atcgcgcgtc
    15901 ccaactcggg atcgaagtcc cggccatggc caccgagggc ataggcgtgc ggatccaggc
    15961 ccagaacctg atagaggtgg cgtatccgct ccatctcgtt ggtctgtgcc gtggtcggcc
    16021 aggtgacggt cgtggtctgg tggagccgcc ggttcggtgc ggtaccgtcg gcgtaggtaa
    16081 aacgcgcgag acgcacgcag tcgaaatacg gcttgaagcc ctcgcctgcc acacgcatcc
    16141 ggatcgcgta cacgcccttg gcctcgaacg cgctttcggg aagggggggc agcgtcatct
    16201 cgccctcggc cagatcaaat gcctgttcgc ctttccagac gtcgcgtccg aagaaatcgt
    16261 ggaccgtgat ggcgatcgag cctttgcagg agttcgggcc gcgcaccacg aaccgcgcac
    16321 ggaatgccgt tcccggcggc gcgtagaagg agtccggcgc atctgttttg agttcgatgc
    16381 cgattggatt tcccttgtat gccgtcgctt ccgcgccctc ctcgaactgc acatcgtcga
    16441 tgaggacacc cggatccgcg ttgatctgga aagcgattgc gcggaagggc cagttggtga
    16501 cggtgtaggc atagcgtttc cactcctggg agtacccgaa ccatttcggt ttcgtgggat
    16561 tgttccagag gtaggtgtcg cagtggatgc tgccgatgca acggttggtg acggtcgcgc
    16621 ggacgtaaca gctgaaggta taatccctgc ccgcaagggt cggaaagttg aaggtcttgg
    16681 ctcccccgca cgcgagcgcg aaccggccgg aacgcgcgtc gacctccgtg atgtgcgggt
    16741 agagatccat gccggagggg acattgtggt agagacgcgg gccggactcg aaggacgggt
    16801 tcacgagcag gttgccccgt tcgtcgtaac acgggacgtc gtagtcgttg ttgagcgtga
    16861 agttgatgcc gttgacgacg cactgcggtt tagcggtccg ggcgctcctg ccgaaatcga
    16921 ggatgaccga gaactcctcg gtccggcaac gcggattcca actgaagtaa agctccgctg
    16981 tgccgtcgga acggaaaagc gtcaggcttt cgcaggtgtc gggggggaac tcgagcgcga
    17041 aggcttttgc gggatgttcg gggtcgaact cgatccgcga gggtttcgca acatggaagc
    17101 agttgatcca gacagacttc gcgccgaggg tccacttcgt attcgtgtcc ggaaacgtgt
    17161 aaacgcgttg cccgtctttc ccgcaaaccg cgagctggcg tccgaaacag gtcggcttgg
    17221 gaatcgagaa cacgagcgtt ggtcccttga gtgtcgcgta atcctccatc tcgtatcgga
    17281 gcgtgaactc cgccttgccg tcgggccgga gaaagatgcg ctggcggaac gtgccgcccg
    17341 cacttggggt ggtcttgaac gggcagcttc gctcaatgcc tttttgtggg tcgtaagtgg
    17401 tgtcgaacgt ggtgaagggg ttccatccgg cctcggggga ggcatgcatg cgcgttttga
    17461 gaacctcctg ttcgtcgatc agcagctgca ggggggagag tccgccgcgg acaccggaat
    17521 tccagatgag cgccttcgat ccggagacga tcttgtccga aaacatcact gcttccggct
    17581 ggtagagttc ggcggcgtcc gctgcgaacg gagcctgcgg cacggcgatt ttgctcgcag
    17641 ggagcgtgtc gggacgattt acgcaacttg tggccagcgc caggattgtg gcgaagttca
    17701 agaacgtgct gatccatttc atttttttcg ctgcccccat cgggttccac ccttaaagat
    17761 ttcatattac cataagagga atgagaagcg ccaaccatga aacatcatga
//
LOCUS       DAOSLE010000051        17684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3414_length_17684_cov_9.287084, whole genome shotgun sequence.
ACCESSION   DAOSLE010000051 DAOSLE010000000
VERSION     DAOSLE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17684
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_17684_cov_9.287084"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(481..2742)
                     /gene="rnr"
                     /locus_tag="PK576_05130"
     CDS             complement(481..2742)
                     /gene="rnr"
                     /locus_tag="PK576_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOE61009.1"
                     /translation="MNKFDKEKAAIQDLFESGPERAWTAKEISRTLGLRGKQINRLHD
                     RLGEMVRDGVIVQIRRGNAYTLGKQADLVTGPLRTVRNGAGYVTERESGKSIWVESED
                     LGTALPGDLVTIRLYRNGVERKGKVIRIVERSKRDIVGTLMTTGRFLHVVPLNPVYRK
                     NFYVPAANGAREGDRVVVRFTDWKNRYVAPEGEIVDVIGPADKPSLDTLVVMKQFDLP
                     EEFPPEVIDEAEKVARRLSRPGKREDLRSRYIITVDPATARDFDDAVSLESDEHGNRV
                     LGVHIADVSHFVRPGAPLDREARERGTSVYLVDKVIPMLPEQLSNGVCSLRPDEDRLT
                     FSAFMTFDASGRMVARRFAKTRIRSKLRLSYEQAMDILSDRKPKGLASVPKPARALLK
                     GARRLAEQLRARRFSMAALDLEVPEVEVVLDEQARMTGLVTRPYDASHQLIEECMIAA
                     NEAVATELWTRGIKILARLHEPPDGEKLEELQASLAILGFRPGDLSNPKHLARFLTDT
                     REHPLRYHAHMMVLRSMKRAVYSAEKIGHFGLAKTYYAHFTSPIRRYPDLLLHRQLAD
                     FLDGKGGRMPQHELVQTAFLATEREQVADDASRQLIEIKKFRFLQQQLDERKPIPYRA
                     VVVKCTNYGVFIDIPGLALGGLVHISNLSRQFVRFNPANESLSAGQTVYRVGTVLQVQ
                     VASVDFNQRHADFMLVGGEASGERGDGTEQPRKGGARRSAGEKRGRQSDTAGRRPRGE
                     PRPAKSGRRRKGR"
     gene            complement(2798..3910)
                     /locus_tag="PK576_05135"
     CDS             complement(2798..3910)
                     /locus_tag="PK576_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE61010.1"
                     /translation="MNLAAHLAALPPAEASGLRAVAERLRQLPEREPSADLTERILRA
                     VNTEHSRPPFRAPKLWRVAAVLVATLALVALYLRFGVPAVRDAQADDGSCWIAAAQEA
                     DGTWQPARHGGAEAYRPALTALSALALDRADGRKYADPIGRACKALAALQNEDGAFGG
                     NGRERYYNHAMTTFALAALASDRPGLKPVLERALAFSRASQTVEGGWDYEPGSDGNAA
                     LTSWQVRALAEMRRIGFAEAEIPLRKGLRWLRGTLREDGHVAYHRGTRNPSECLDALT
                     AHTLITAGGAYAGLADLGRQVAGRLNPAEPEDVADCYRDYAKTLAFAAAGAAEQAARV
                     SRQMIAHHQAGARDQWERVGGRLYTAALTRLAVGAL"
     gene            complement(3907..4467)
                     /locus_tag="PK576_05140"
     CDS             complement(3907..4467)
                     /locus_tag="PK576_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOE61011.1"
                     /translation="MSAYGQREEMEDAALMLQAGAGDNEAFAVLVRRHQKKLLNYFTR
                     TGVQYDCEDLVQQTFLRLYRYRNRYRPTAKFTTFLFLLARQVWVDELRRRKRQERLAK
                     GLAAEPHPEYAAPVAADAGASGGLDLAQALAGLPEGLRQVVELGVYQDLPYAEIAAIL
                     KIPVGTVKSRMFTALARLRAHWEEKP"
     gene            complement(4474..4971)
                     /locus_tag="PK576_05145"
     CDS             complement(4474..4971)
                     /locus_tag="PK576_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOE61012.1"
                     /translation="MISILKGAGVWDYLAFAGVAFLVIRGFVRGCSGEMGRLVGTVTA
                     AAVGYFGFRPIANLVLASPVFGANPYAGRLVTFILMAVLCIAIWFGLSYLLTELIRLA
                     IAQPFDGILGGIIGGIKALVVVAAFVAWGLLNPKEEERVRLTEQSLTARWLAPLLKNI
                     TGPDG"
     gene            complement(5006..6163)
                     /locus_tag="PK576_05150"
     CDS             complement(5006..6163)
                     /locus_tag="PK576_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61013.1"
                     /translation="MIRTVWLLVLVWFGAGLGCGTDSGQKALKQGLAAYKEKNYAAAV
                     THLNRAGKRLTGSVPLYYHLGLAHLHQGEIEPAQAAFAAALELAPGHAASKVCLGQIA
                     YLQNDLAKAQTCFDEALAVTEAPEERARILTALGLTEMGRNRNDLARLYLIRAQQAHR
                     AYAPAYYNLASLYRDAFNLREEALDQFELYVRLADRDDRHVEKAENNIKRLRLNLTRI
                     REEASEGVRRDPATAARLLQEGARLHADRQSAKAVKAFRDALAADPLTFSAAYGLAMA
                     YKAQGRRAEAMEAFRRAAQINPDHQDSYHQAAELAFQLKRYVEAGKLLDRAIARSPFQ
                     VASAELMARIRHAEGRLSEARAYGEYTLSLMHPNAKGRRAYEQWVRSLPAR"
     gene            complement(6236..10300)
                     /locus_tag="PK576_05155"
     CDS             complement(6236..10300)
                     /locus_tag="PK576_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE61014.1"
                     /translation="MRRRWGIGIAVVLLLGAGAGADEVYLTDLDISKTRQGWKEATRD
                     FNLFGARLQVAGRSYTNGMATCSPSELYVELNGARRFLAEVAVDDTSDQPDKRLVFQV
                     IGDGRLLWQSTPMRRGDRPVPVEVEVSAFTTLLLRVDPWRDGTAGDHADWLNARWVTD
                     GSRKPRAREPLNWPKAWLSTLDASFVTPDGAAVTSLPLRVAGVMYAEGLGMRVPSELC
                     VLRGSAARFAGKVGVDDAGADGSAVCMLYAGDRVLWSSGTMRCGEPARAFDIPIEEAG
                     QVRLVTTGDAGAQVDLVETLFTLTGRLRPCAAYNPAVYAKRPDWENPLVFRVGTEKPA
                     ATMWVFDSIRAARDATAREESPFFLSLDGVWKGHWVPHPDQRPQGFQQPDYPDGGWRD
                     LTVPGCVELAGLGTPLYKNIGYYFKVDPPFVLGEPDSRYTTHRERNAVTSYRRTFTLP
                     EDWEGRTVYLRFDGFASAMAVWLNGAKLGYAEDGRQGATFNVTAALRAGVNTLAVEVY
                     RLCDGSYMEDQDFWRLSGLCRPVYLWSVPKTHVRDVFVRTLPAVAGEFAGTWNLTVEA
                     EIAEGGDTDARLEAALFAYRFRNGFWNRTVAHGVAEAAGAGFRLNLPVVTPRLWSAEQ
                     PNLYTLVVTLRDARGRILQTIPQRVGFRQVEWDRGQLRVNGQPVLFKGVNRHEMDPDH
                     GYAVPRERMIQDITLMKRHNINAVRTSHYPNDPRWYDLCDAYGLYVMDEANLETHGLA
                     NTPRNPVVDPSYRAAALDRQMGMVERDKNHPSVIIWSLGNENEVDSDFFAEAYALIRA
                     RDPGRPIQNQRNGPRDFENTMYTRVRDLEAYGQRTDTERPFILCEYSHAMGNSSGNLA
                     EYWRVIESYPSLQGGFIWDFADQALRKPLPPERVRHGEETYFWAYGGDYGDFPNDDNF
                     NCNGLVQADRRPTPQLAEVRHVYQNVSVTARDVTRGLFTVHNKAFFTSLREVACRWTY
                     EENGEVIDSGSLGRLDVPPRGSLDFALPLSMVRRPVYAARVSTWNFRFLTLGDTPWAE
                     KGFEVARDQVVVPAEVQPTLMTATAGRAPVRLEETDREVTATGQDFDVRISKATGSIL
                     SWRVCGEEQILSPLEPDFWRAPTDNDRGNQMAARHAIWRDAAARREVRGVSLRQEVDG
                     NWRILVRLAFPSAGATSGTLHYSFTPAGQIRVAFKVTPAGVNLPSLPRVGMTLQIPLA
                     YDRVTWLGRGPHECYADRQASAFFGLYTCSLADLFFPYVEPQESGNRTDTFWVTFTDA
                     AGKGIRVTGDPKINFSARLYTTRELETRKHPWELTASGNGVVNLDYGQMGLAGENSWG
                     ARPWPEHQLSPGREYSYGFVLEPVSVIAAQRRLR"
     gene            complement(10438..11679)
                     /locus_tag="PK576_05160"
     CDS             complement(10438..11679)
                     /locus_tag="PK576_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE61015.1"
                     /translation="MSEQQQASLRTRVVLSALYFLQYMALPVWFITLVPYAKSMGAQG
                     SMLAWMVSAMAIGTLASPFVGMVTDRFFAAQKVMAFLNVVSAVGLVCAGLQTKPLPLL
                     IILTVTMVFYMPTWSTINYIIFSHCSPPVFLQIRVFGVIGWIAAAAFSVVGDWIGVKV
                     NGTPLMFYCGAATVLVAGLLALLVPNTPPKARGQPMSLVDVLGLRAISMLRDPSFALF
                     IAIMFFSKVPFSLYFGYGGEFLADSGFKNPTLSMSFGQYSEIFFTLALASTIKKIGAK
                     NAMMIGLAALFLRYVFFWLGGPVGIYSGILIHGLIFGFFYVGGQIYVDSRADGAMRAQ
                     AQGFINFAAFGLGTFFANFVNRALIDGLRVTTTGADGVVHSNWSRIWLVSMILSAVLL
                     LIFALFCRSEDKPVSPDTQAA"
     gene            complement(11692..12564)
                     /locus_tag="PK576_05165"
     CDS             complement(11692..12564)
                     /locus_tag="PK576_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE61016.1"
                     /translation="MDKVKPQDLRVMIIGAHPDDPDITGGGLALNFVKVGATVKLVSM
                     CNGDKGHMTMSSEALAARRYQEAQRSKDILGVDEYRVLNHHDCELEVTLEMRKEVTRF
                     IREFAPHLVITHRTCDYHADHRAVGTLVMDSAYLLGVPLWCPETPIPAVKPAIYFLRD
                     AFTQPSEIRPDIVVDIEDEMDQLLTALCSHESQFFEWLVFDKGIAEPVPTSLEGRKEF
                     LKTYWMLPRKAADAVRFRARLTEVYGKERAKEIGFIEVYELSEYGYQPTADEVGWLFP
                     FVPGCASATGVPKR"
     gene            complement(12595..13416)
                     /locus_tag="PK576_05170"
     CDS             complement(12595..13416)
                     /locus_tag="PK576_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE61017.1"
                     /translation="MRQCKKWPIGICSWSLKTGVEEIGLVMTALGLEHMNLAIAPALA
                     PDGEAYLDAVRRQGWTITAGMMNYTYEDYSTLDTIRRTGGIAPDEHWPASGEAFGKAA
                     KITAELGSPYIMMHVGFLDHTDTAYAKKFYDRVRYLGDRAGEAGVSLLMETGQETAED
                     LQRFVETLNHPNVLLNFDPANLILYDKDEPIGAFRRLAPWVRHIHIKDGIRTATPGTW
                     GRKTFWGDGEVNAFAFLKALEDEGYDGALAIEYEANSLQERVAAITTAVRRLEAY"
     gene            complement(13445..14479)
                     /locus_tag="PK576_05175"
     CDS             complement(13445..14479)
                     /locus_tag="PK576_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61018.1"
                     /translation="MIKVAIAGFGFMGRMHYGCWKKVPGVQVVALCDQDRRQFEQSAA
                     AAGNVQGADMSCDYGDAVLYDDFATMLDQARPDAVDITLPTFLHVSTAALALSKGIHV
                     LCEKPMALDAAACDELLAAWRAAPQGTELMIGQCLRFRGEYVYAKRLIESGEYGRVIA
                     ARFGRYSQAPGWTRDGGKSWFFDESRSGGVALDLHIHDTDMVHFLFGMPAAVTSSAYY
                     DSDGTMIHISTLYDVGGPSVVADGGWGMAKSFGFERGFQIAFERASLVMATNRQPEFC
                     LYPMDGGPFVPELPVSTDSYQNEINWFVDKLKGLPVEAVTTPEACRDSVKIVDAEKLS
                     ARGKKPVQIV"
     gene            complement(14576..15976)
                     /locus_tag="PK576_05180"
     CDS             complement(14576..15976)
                     /locus_tag="PK576_05180"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61019.1"
                     /translation="MSLARVSRRRFAAAAGAVCAFPNLIPSSAFGQKAGRPAPSGRVN
                     VGLIGCGVQGITGNLNNFLHDDNTQVVSVCDPVSEAPLYGYRGQLQGGREPARWQVND
                     FYAKKNNKPGYDGCKVFADFRAMLEDKDLDAVCVATPDHWHALIGIMAARKGKHIFGQ
                     KPLANSVAEGRALCEAVKKHGVVFQTGAQGRSSDPNQRRAAELIRNRRLGKLQRIQLG
                     LPGGMPAWGKTAEQLSERPLTRPPAYVDFDLWLGPAPQSPYIPKLHLPMQWRFNFQYG
                     GGMVTDHGAHFIDILQWSLGMDESGPVAIENIRGEMDKPDSLYNVASRFSFEIVYEDG
                     TRVHTSTDFGENPSRFLRYFGENGRELYICGGKLETKPFDLIREKPRDGEVKLYVSSN
                     HERNFIEGILTGRPVISPCETGHRTITIAHLANIGLRLGRKDLKWNPRAERFVNDPEA
                     DAMLGVAYRAPWTLEG"
     gene            complement(15988..16314)
                     /locus_tag="PK576_05185"
     CDS             complement(15988..16314)
                     /locus_tag="PK576_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61020.1"
                     /translation="MSFPVFEAMMLICFGAAWPVSILKSWRSRTNRGKSLFFMVIVFA
                     GYIFGVCHKLFWQERVDGAVWLYLFNLVMVGTDLVLYYRNHRIDKAQADAPLYTAQRG
                     DLTQGR"
     gene            complement(16619..16951)
                     /locus_tag="PK576_05190"
     CDS             complement(16619..16951)
                     /locus_tag="PK576_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61021.1"
                     /translation="MTKKGSGVVWGRVCMKGWPMRVNKRGLFGLACLSLLFVAEVSAH
                     DIQVSRVRLTDWDEARQCVMVTFDLAWNNAWRDAGNWDAAWVFVKVKPAGSNDWRHAT
                     LSPEGRVR"
     gene            17345..>17684
                     /locus_tag="PK576_05195"
     CDS             17345..>17684
                     /locus_tag="PK576_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61022.1"
                     /translation="MVGALAAAVVLKGAYPLLVTPYTEKAELDCEVLIREAEYVDAQG
                     VGGVIWPTANEINVLDEFGEYESGLTALADRWAKGGMKSRLTAVCCGTNSAAALKRVE
                     LWDAIVSKRGI"
BASE COUNT         3540 a         5923 c         4987 g         3234 t
ORIGIN      
        1 tgaaggttca actgctcttt ttaggttaag caatcgatcc cgatagcgat cccgatttgg
       61 atgaaaccat gaaaaacatg aaacttcatg aaaatcagga attccccctg gcgttccaaa
      121 aaaacccgag cgatgcccca aaaaatctgc gtaatctgtg taaatctgtg gataaaaacc
      181 cgagcagtgc catcccgtga gttcatggta aacagtcgat cccgatagcg accccgaccc
      241 cgatttggat gaaaccttga aaccttgaaa ccttgaaacc ccgaaaccct gaaacccacg
      301 aaagtgtccc gtggtaaaac agtcgatccc gatagcgatc ccgatcccga tcccgatttg
      361 gatgaaacca tgaaaccttg aaacctgaaa ccaaagagag ttgaaggttc aactgctctt
      421 tttggtttaa atgatgaata cggatggccg atccggcacg atgagtgtgc ccaaaccgcg
      481 ctatctgccc ttgcgccggc gtccggattt cgccgggcgc ggttccccgc gcggacgccg
      541 ccctgccgta tcggactggc gtccgcgttt ctccccggca gatcgccgcg caccgccttt
      601 gcgcggctgc tccgttccat cgccgcgctc cccggacgcc tccccgccga ccagcatgaa
      661 atcagcgtgg cgctgattga agtcgacaga cgccacctgc acctgtagca cggttccgac
      721 acggtaaacc gtctgacccg cgctcaggct ttcgttcgca ggattaaagc ggacaaactg
      781 ccgggagagg ttggagatgt ggaccaagcc gcccagagct aacccgggga tatcgatgaa
      841 caccccgtag ttggtgcact tcaccacgac ggcgcggtac gggatcggct tccgctcgtc
      901 cagttgctgc tggagaaaac ggaacttttt gatctcgatc agttggcggg aggcgtcatc
      961 cgcgacctgt tcgcgctctg tcgccagaaa cgcggtctgc accaattcgt gctgcggcat
     1021 tcgtccgccc ttgccgtcca gaaaatcggc gagttgccgg tgcaggagta gatcgggata
     1081 ccgccggatc ggcgaggtaa agtgcgcata gtacgttttg gccaatccga aatggccgat
     1141 cttttcggcg gagtagacgg cgcgcttcat actgcgcagc accatcatgt gggcgtgata
     1201 gcgtagcggg tgttcccgcg tatccgtcag aaaccgggca agatgcttgg ggtttgaaag
     1261 atccccgggg cggaaaccga ggatcgccag actggcctgc aactcttcga gtttctctcc
     1321 gtccggcggc tcatgcagcc gcgccagaat tttgatgccg cgtgtccaca attcggtcgc
     1381 gaccgcctca ttggccgcga tcatgcactc ctcgataagc tgatgggacg catcgtaggg
     1441 gcgcgtcacc agaccggtca tgcgcgcctg ttcgtccagc accacctcga cctcgggcac
     1501 ctccaaatcc aacgccgcca tggagaaccg ccgcgcgcgt aattgctcag ccagccgtcg
     1561 cgcgcctttc aggagcgcac gggccggttt gggcacagag gccagccctt tcggtttccg
     1621 gtccgaaagg atatccatgg cctgctcata actcaaccgc agtttggagc ggatccgcgt
     1681 tttggcgaaa cggcgggcga ccatgcggcc cgaggcgtcg aaggtcatga acgccgaaaa
     1741 ggtaagccgg tcctcatccg gccgcagact gcacacgccg ttggaaagct gttccggcag
     1801 catggggatc accttgtcca ccagatacac gctggttccg cgttcacgcg cctcccggtc
     1861 cagcggtgcc cccggccgga cgaaatggct gacatccgcg atgtggaccc ccagcacccg
     1921 gttgccgtgt tcgtcgctct ccaacgagac ggcatcgtcg aagtcacgcg ccgtggcggg
     1981 atccaccgtg atgatgtagc gcgagcgcag atcctcgcgt tttccggggc ggctcagacg
     2041 ccgcgccact ttttccgctt cgtcgatcac ctcgggcgga aactcttcgg gcaggtcaaa
     2101 ctgcttcatc accaccaacg tgtcgagcga cggcttatcc gcgggcccga tcacgtccac
     2161 aatctccccc tcgggtgcga cgtaccggtt tttccagtcc gtgaagcgca ccaccacacg
     2221 atcgccctcc cgggcaccgt tggcagcggg aacataaaaa ttcttacggt acaccgggtt
     2281 cagcggcacc acatggagaa agcgtccggt cgtcatcagc gtgcccacga tatcccgctt
     2341 cgaccgctcg acgatccgga tgaccttgcc cttccgttca acgccgttgc ggtagagacg
     2401 gatcgtcacg agatcaccgg gtagcgcagt gccaaggtct tcggattcga cccagatgct
     2461 ttttcccgac tcgcgttccg tgacgtaccc cgcgccgttc cgcactgtgc gcaacgggcc
     2521 tgtgaccaga tccgcctgtt taccgagcgt gtaggcgtta ccgcggcgga tctgcacgat
     2581 cacgccatca cgaaccatct cgcccagccg gtcgtggagt cgattgatct gcttgcctcg
     2641 caaccccaga gtccggctga tctccttggc tgtccaagcc cgttccggcc cggattcgaa
     2701 caagtcttga atggcggctt tctctttgtc gaatttgttc atcggagtca gtgtacctga
     2761 tccgccgcac ccgcgtctgt agaaaaaaca tgcgtcacta cagggcgccg accgcgaggc
     2821 gcgtcagggc ggcggtatag agccgtccgc cgacacgttc ccattgatcg cgcgcgccgg
     2881 cctgatggtg cgcaatcatc tgacgggaaa cccgtgcggc ctgttccgcc gcaccggctg
     2941 cggcgaacgc caacgttttg gcataatccc gataacaatc cgcaacatcc tccggttcgg
     3001 ccggattcag ccgacccgca acctgtctgc ccaagtcagc caagccggca taggcaccgc
     3061 ccgctgtgat cagagtgtgg gcggttagcg cgtccagaca ctccgacggg ttgcgcgtcc
     3121 cccggtgata cgccacatgt ccgtcctcgc gcagtgtgcc gcgcagccag cgcaaccctt
     3181 tacgcagcgg gatttccgcc tccgcgaatc cgatccgccg catctccgcc aacgcgcgca
     3241 cctgccacga ggtcagagcg gcgttcccgt cggagccggg ttcgtaatcc caaccgccct
     3301 cgaccgtttg gctggcgcgg ctgaaggcca gcgcacgctc caaaaccggt ttcaggccgg
     3361 gcctgtcgga agccagcgcg gccagcgcga aggtcgtcat ggcatggttg taataccgct
     3421 cgcgtccgtt gccgccaaag gcaccgtcct cattctggag ggcggcgagc gccttgcacg
     3481 cgcgcccgat tgggtcggcg tactttcgtc catccgcacg gtcgagcgcc agggcggaga
     3541 gggcggtcag cgcgggacgg taagcctcgg cgccgccatg tcgggcgggc tgccatgtgc
     3601 cgtccgcttc ctgcgctgcg gcgatccagc acgatccgtc gtccgcctgc gcgtcgcgca
     3661 cagccgggac gccgaaacga aggtagagcg cgaccagcgc gagcgtcgcg accagcacgg
     3721 ccgcgacccg ccacagcttg ggcgcacgga aaggcggacg ggagtgttcc gtgttcacag
     3781 cacgcaggat ccgttccgtc aggtcggccg acggttcgcg ttccggcagt tgacgcagac
     3841 gttccgcgac agcgcggagc ccgctcgcct ccgcaggcgg caacgcagcc aaatgtgcgg
     3901 cgagattcat ggtttctcct cccaatgcgc ccgcaatcgc gcgagcgcgg taaacatgcg
     3961 cgacttgacg gtccccaccg ggattttcag aatcgccgcg atttccgcgt acggcagatc
     4021 ctgatagaca ccgagttcga ccacctgccg gagaccctcg ggcaatccgg ccagcgcctg
     4081 cgccaaatcc agaccacccg acgcgcccgc gtcggccgca accggcgcag cgtattccgg
     4141 gtggggctcc gccgccagac ctttggccag ccgttcttga cgcttgcgcc gacgcaactc
     4201 atcaacccac acctgccgcg cgagcagaaa gagaaaggtt gtgaacttcg cggtcggccg
     4261 atagcggtta cggtaacggt atagcctcag gaaagtctgc tgaaccaaat cctcacaatc
     4321 atattgaacg ccggtacgcg tgaaatagtt caggagcttt ttctgatgcc gacggaccag
     4381 caccgcaaag gcttcgttgt ctccggcgcc cgcctgcagc atgagggcgg catcttccat
     4441 ctcttcccgt tgcccgtatg cgctcatacg cccctatccg tcgggaccgg tgatattttt
     4501 cagcagcggg gcgagccacc gtgcggtcag cgattgttcc gtcaaccgga cgcgctcctc
     4561 ctctttagga ttgagcagtc cccaagccac gaaggccgcg accaccacca gcgccttgat
     4621 gccgccgatg atgccgccca gaatgccgtc aaacggctgg gcaatggcca ggcggatgag
     4681 ttcggtgagc agataactca atccgaacca gatcgcgatg cagagcaccg ccatcaaaat
     4741 aaaggtgacc agacgtccgg cataggggtt ggcgccgaac accggcgagg cgagcaccag
     4801 gttggcgatc gggcggaatc cgaaataccc gaccgcggcg gcggtcacgg ttcccaccag
     4861 acgtcccatc tcaccggaac agccccgcac aaacccgcgg atcaccagaa aggccacacc
     4921 ggcaaacgcc aaatagtccc aaacgcccgc ccctttgagg attgagatca taacaccgtc
     4981 ccgtccgcct gccgtacccg tgtactcatc gggccggcag cgaccgcacc cactgttcgt
     5041 acgcgcgccg ccccttcgcg ttcggatgca tcagcgagag ggtatactca ccgtacgcgc
     5101 gcgcctcgga caaccttccc tccgcatgcc ggatccgcgc catcaattca gcgctggcga
     5161 cttgaaaggg gctgcgcgcg atcgcgcggt ccaacagctt ccccgcctcg acgtagcgtt
     5221 tcaattggaa agccagttca gcggcctgat gatagctgtc ctgatgatcg gggttgatct
     5281 gcgcggcacg ccgaaaggcc tccatggcct cggcgcgccg accttgagcc ttgtatgcca
     5341 tggccagccc gtacgccgcg ctgaacgtca gaggatcggc ggccagcgcg tcacggaagg
     5401 ccttgaccgc cttggccgac tgccggtcgg catgcagccg ggcgccctcc tgaagcaggc
     5461 gtgccgcagt agccggatcg cgccgcacac cttcgctcgc ctcctcccgg atccgggtga
     5521 ggttgaggcg cagccgtttg atgttgttct ccgctttttc gacatggcgg tcatcccggt
     5581 cagccagcct cacatacagt tcgaactgat ccagcgcctc ttcccgcagg ttgaaggcat
     5641 cgcggtagag actggcgaga ttgtagtacg ccggagcgta ggcgcggtgg gcctgctgcg
     5701 cgcggatcag ataaagacgc gcgagatcat tccggttacg ccccatctcg gtcaaaccga
     5761 gcgcggtcag gatccgggcc cgctcctccg gtgcctcggt cacagcgaga gcctcgtcga
     5821 aacaggtctg cgccttggcc agatcattct gcagataagc gatctgcccc agacagactt
     5881 tggacgcggc atgacccggt gccaactcca gcgccgccgc gaaggccgcc tgcgccggtt
     5941 cgatttcccc ctgatgcaga tgcgcgagcc ccagatgata gtacaacgga acggatccgg
     6001 tgagccgctt gccagcccgg ttcagatgcg tgaccgctgc cgcgtaattc ttctctttgt
     6061 acgcggccag cccctgcttc aaagctttct gcccggaatc ggtgccgcac ccgagtcctg
     6121 cgccgaacca aaccaaaacg agcagccata ctgtgcgaat cataacggac cacctcacca
     6181 cgccccgcag ccgccacccg acgaccggac cgggaacagt aaaaccgttt gtatattacc
     6241 gaagacgccg ttgcgccgca atcacggaaa cgggttccag aacaaaaccg tagctgtatt
     6301 cgcgtccggg cgagagttgg tgttcgggcc aaggacgcgc cccccaactg ttctcgccgg
     6361 ccaaacccat ctgtccgtaa tccagattca ccaccccgtt accgctggcc gtgagttccc
     6421 aggggtgttt gcgcgtttcg agttcacgcg tcgtgtagag ccgcgcgctg aaattgattt
     6481 tgggatcgcc tgtgacccgg atccccttgc ctgccgcatc cgtgaaggtc acccaaaagg
     6541 tatccgtgcg gttgccgctc tcctggggct cgacatacgg aaagaagaga tcggccagag
     6601 aacaggtgta caagccgaaa aaggccgaag cttggcgatc agcataacat tcgtggggtc
     6661 cgcgcccgag ccacgtcacc cggtcatacg cgagcgggat ctgcagcgtc atccccacac
     6721 gcggcaacga tggcaggttc acgcccgcag gcgtcacctt gaacgccacg cggatctgcc
     6781 ccgccggggt gaacgagtaa tggagtgtgc cggaagtcgc gcccgctgag ggaaaggcca
     6841 gacgaaccag aatgcgccag ttgccgtcca cctcctgccg gagactgacg ccgcgaacct
     6901 cgcgccgcgc cgccgcatcg cgccagatcg catggcgcgc cgccatctga ttgccgcggt
     6961 cgttgtcggt cggcgccctc cagaaatccg gctccagcgg cgacaggatc tgctcctcac
     7021 cgcacacccg ccacgagaga atcgaccccg tggccttgct gatccgcacg tcaaagtcct
     7081 gtcccgtcgc ggtgacctcc cggtccgtct cctccagccg caccggcgca cgtcccgcgg
     7141 tcgcggtcat cagggtcggc tgcacctcgg cgggcaccac cacctgatcg cgcgccacct
     7201 cgaagccctt ctccgcccaa ggggtgtcgc cgagcgtcag gaaccggaaa ttccaagtgc
     7261 tgacccgcgc ggcgtaaacc gggcggcgca ccatagagag cggcagagcg aaatccagcg
     7321 atccccgagg cggcacatcg agtcgcccga gactcccgct gtcgatcacc tcgccgttct
     7381 cctcgtacgt ccaccggcac gccacctccc gcagcgacgt gaagaacgcc ttgttgtgga
     7441 ccgtaaaaag cccgcgcgtc acatcgcgcg ccgtcaccga gacgttctga taaacgtgcc
     7501 gcacctccgc cagttgcggc gtgggccgcc ggtccgcctg caccaatccg ttacagttga
     7561 agttgtcgtc gttcggaaaa tccccgtaat cgccgccgta tgcccaaaaa tacgtctcct
     7621 cgccatgccg cacacgctca gggggcaacg gcttgcgcag ggcctgatcc gcgaaatccc
     7681 agatgaaccc gccttgcagc gaggggtagg actcgatcac ccgccagtac tcggcgagat
     7741 tcccgctgga attgcccata gcatgcgaat actcgcacag gataaagggc ctctccgtat
     7801 cggttcgctg accgtaggcc tccagatccc gcacacgcgt atacatggtg ttttcaaaat
     7861 cgcgcggccc gttgcgctga ttctggatcg gccgtcccgg atcgcgcgcc cggatcaggg
     7921 cgtaggcctc cgcaaagaaa tcggaatcca cctcgttctc gttgccgagg gaccagatga
     7981 tgactgaggg atgattcttg tcgcgttcga ccatgcccat ctgccgatcc agcgctgccg
     8041 cgcggtagga ggggtcaacc acgggattac gcggtgtgtt ggccaacccg tgcgtttcca
     8101 aattggcctc gtccatcaca tacagcccgt acgcatcgca caggtcgtac caacgcgggt
     8161 cattgggata gtggctggtc cggaccgcgt tgatgttatg ccgcttcatc agtgtgatgt
     8221 cctgaatcat gcgctcccgc ggtaccgcgt acccgtggtc cggatccatc tcatggcggt
     8281 tcacgccttt gaacagcacg ggctggccgt tcacccggag ctgcccgcga tcccattcaa
     8341 cctgccggaa cccgacgcgc tgcgggatcg tctgcagaat ccgtccgcgc gcgtcgcgca
     8401 aagtcactac cagagtatag aggttgggtt gttccgccga ccacaaacgc ggtgtcacca
     8461 ccggcaggtt gaggcggaat cccgcaccgg ctgcctccgc cacgccatgc gccacggtcc
     8521 ggttccagaa accgttccgg aaccggtagg cgaacaacgc cgcctccaga cgcgcgtccg
     8581 tgtcgccgcc ctccgcgatc tccgcctcaa ccgtcagatt ccaagtccct gcaaactccc
     8641 ccgccactgc cggcagagtc cgcacaaaca catcccgcac atgggttttg ggaacggacc
     8701 agaggtagac cgggcggcag agacccgaca aacgccagaa atcctgatcc tccatgtagg
     8761 agccgtcgca caggcggtat acttccaccg ccagcgtgtt cacccccgcc cggagcgcgg
     8821 ccgtcacgtt gaacgtcgcg ccctgacgcc catcctccgc ataccccagt ttcgcgccgt
     8881 tgagccatac ggccatggcc gaggcgaagc cgtcaaaccg cagatacacg gtgcgtccct
     8941 cccaatcctc cggcagggtg aacgtgcggc ggtaggaggt caccgcattg cgctcgcggt
     9001 gggtggtgta ccgcgaatcc ggctcgccca acacgaacgg ggggtcaacc ttgaagtagt
     9061 agccgatgtt tttatagagc ggcgttccca gtcccgcgag ttccacgcat cccggcaccg
     9121 tcagatcgcg ccaaccgcca tccggatagt ccggctgctg aaacccctgg ggccgctggt
     9181 ccggatgcgg tacccaatgg cctttccaca cgccgtcgag cgagagaaag aaaggggact
     9241 cctcgcgtgc cgtagcgtca cgcgccgcac ggatggagtc gaagacccac atcgtggccg
     9301 ccggtttttc cgtgccgacc cggaacacga gcggattctc ccaatcggga cgcttcgcat
     9361 aaacggccgg attgtaggcc gcgcagggcc gtaggcgacc cgtcagcgtg aagagcgtct
     9421 ccaccaaatc gacctgtgcg cccgcgtcgc ctgtggtcac cagccgtacc tgtcccgcct
     9481 cttcgatggg gatgtcgaag gcccgggccg gctcaccgca ccgcatggtg ccgctgctcc
     9541 acaatacccg atcccccgca tagagcatgc agaccgcgct tccgtccgcg ccagcgtcgt
     9601 ccacaccgac tttcccggca aagcgcgcgg ccgaaccccg gagcacacac agttccgaag
     9661 gcacccgcat cccgaggcct tccgcataca tgacacccgc cacccgcaac ggcagagagg
     9721 tcaccgccgc gccatcgggt gtcacaaacg atgcgtccag cgtggagagc caggctttcg
     9781 gccagttcag cggttcccgc gcgcgcggct tacgcgaacc gtccgtcacc caccgcgcgt
     9841 tcagccaatc ggcatgatcc ccggccgtac cgtcccgcca cggatccaca cgcagcagga
     9901 gcgtggtgaa cgctgacacc tccacctcca ccggtaccgg ccggtcgccg cgccgcatcg
     9961 gcgtgctctg ccacagcagg cgcccatcgc cgatcacttg gaaaacgagg cgcttgtccg
    10021 gttgatcact ggtatcgtcg accgccacct cggcgagaaa ccggcgcgcc ccgttcaact
    10081 cgacatagag ctcggaaggc gaacaggtcg ccattccgtt ggtgtagctc cgtcccgcca
    10141 cctgcaaccg cgcgccgaag aggttgaaat cccgcgtcgc ctctttccag ccctgacggg
    10201 tcttcgagat gtcgagatcc gtcaggtaca cctcatccgc acctgccccc gcgcccagca
    10261 acaacacgac cgcaatcccg atcccccaac gtcgtctcat cccctgctcc ctttccgcga
    10321 taccgtttcc ttccgcgtcg caccgcgtcc gctatccatt accgtactgt agatcaaccc
    10381 cctcttgtgc ggcaagggac cttttggcaa ccggttggtg gcgcaagtgc gcggaactca
    10441 tgcggcctga gtgtccggag acacaggttt atcctccgag cggcagaaga gagcgaaaat
    10501 aagcagcaac actgcggaga ggatcatgga gacgagccaa atccggctcc agttgctgtg
    10561 gacaaccccg tccgctcccg ttgtggtcac gcgcagcccg tcgatcaacg cacggttgac
    10621 gaagttggca aagaacgtgc cgagaccaaa cgcggcgaag ttgatgaatc cctgcgcctg
    10681 cgcccgcatc gccccgtccg cccgggaatc aacatagatc tgccccccca cgtaaaagaa
    10741 tccgaagatg agcccgtgga tcaggatccc gctgtatatc cccacggggc cacccaacca
    10801 gaaaaagaca taacgcagaa acagcgcggc caacccgatc atcatcgcat ttttcgcgcc
    10861 gattttcttg atggtcgaag cgagcgccag cgtgaaaaag atctcgctgt actgtccgaa
    10921 actcatggac agggtggggt tcttgaaccc cgagtccgcc aggaattcgc ccccgtaccc
    10981 gaaatagaga gagaagggga cctttgagaa gaacatgatc gcgatgaaca gcgcgaaact
    11041 gggatcccgc agcatggaga tggcgcggag cccgaggaca tccaccagac tcataggctg
    11101 accacgcgcc tttggcggcg tgttcggcac caacaaggcc aagagaccgg ccaccagcac
    11161 cgtcgccgcg ccgcaataga acatcagcgg cgtgccgttg accttgaccc cgatccagtc
    11221 gcccaccacg ctgaaggcgg cagcggcgat ccagccgatc acgccgaaaa cgcggatctg
    11281 cagaaacacc gggggggagc agtgcgagaa gatgatgtag ttgatcgtgc tccaggtggg
    11341 catgtaaaac accatggtca cggtcaggat gatcagcagc ggcagcggct tcgtctgtag
    11401 tccggcgcaa accaatccga cggccgacac cacattcaga aacgccatga ccttctgcgc
    11461 ggcgaaaaac cggtcggtga ccatgccgac aaacggggag gccagcgtcc cgatcgccat
    11521 ggcgctgacc atccaagcca gcatggagcc ttgagcgccc atgcttttgg cgtatgggac
    11581 aagggtgatg aaccagaccg gcagagccat gtactgcaaa aaatacaatg cgctcaaaac
    11641 cacccgcgtc cttaacgaag cctgctgttg ttcagacatc cttccctcct tttatcgttt
    11701 cggcacaccc gttgccgaag cacaccccgg aacaaacggg aacagccagc ccacctcatc
    11761 agctgtcggc tgatacccat actcactaag ctcatagacc tcgataaagc cgatctcctt
    11821 cgccctttcc ttgccataaa cctccgtcag gcgggcacgg aaccgaacgg catcggccgc
    11881 tttgcgcggg agcatccagt acgtcttgag gaactccttg cgcccctcca aagaggtggg
    11941 aaccggctcg gcaatcccct tatcgaaaac cagccactcg aaaaactgcg actcgtgtga
    12001 acagagggcc gtgagcaact gatccatctc gtcctcgata tcgacaacga tgtccggccg
    12061 gatctccgac ggctgagtga atgcgtcgcg caggaaatag atcgcgggct tgactgccgg
    12121 aatgggcgtt tccgggcacc aaagcggcac ccccagcaga taggccgagt ccatcaccaa
    12181 ggttccgacc gccctgtggt cggcatggta gtcgcacgtg cggtgtgtaa taaccaagtg
    12241 cggcgcgaac tctcggataa aacgcgtcac ctctttacgc atctcgagcg tcacttccag
    12301 ttcgcagtcg tggtgattga ggacccggta ctcgtcgaca cccaagatat ccttcgaccg
    12361 ctgtgcttcc tgataccgcc gcgcggcgag tgcctccgac gacatcgtca tgtgcccctt
    12421 gtctccgttg cacattgaga cgagcttcac cgttgccccg accttcacaa agttcagggc
    12481 caagcctccg cccgtaatgt cggggtcatc cggatgcgcc ccgatgatca tcacccgcaa
    12541 atcttgcggt ttgactttat ccatgtccca cctcgattac cgcgtttcgc tttttcaata
    12601 tgcttcaagc cgacgcactg cggtcgttat ggccgccacc cgctcttgca gactgttcgc
    12661 ttcgtattca atcgccaacg ctccgtcata cccctcatct tccaatgcct ttaaaaaagc
    12721 gaaggcattg acctctccat ccccccagaa cgtcttacgt ccccatgtcc cgggagtggc
    12781 ggttcttatc ccatctttga tgtggatatg ccggacccac ggcgccagtc gccggaaagc
    12841 cccgatcggc tcatccttgt cgtatagaat gagattggcg ggatcaaagt tcagcaacac
    12901 gttaggatgg ttcagcgttt ccacaaagcg ctgcaaatcc tcggcggttt cctgcccggt
    12961 ctccatcagc agcgacacgc cggcctcgcc cgcgcgatca cccagatacc gcacccggtc
    13021 ataaaatttc ttggcgtagg cggtgtccgt gtgatcaaga aaaccgacgt gcatcatgat
    13081 atacggtgaa cccagctccg ccgtgatctt cgcggctttg ccgaaggcct cgccactggc
    13141 gggccagtgc tcatccggcg cgatgccgcc tgtccgccgg atcgtatcga gcgttgaata
    13201 atcctcgtat gtgtaattca tcatgccggc tgtgattgtc cagccttgcc gacgcacagc
    13261 atccagataa gcttctccat cgggcgcgag cgctggggca atggccagat tcatgtgctc
    13321 tagccctaaa gcggtcatca ccaaaccgat ctcctcaaca ccggtcttca aggaccaact
    13381 gcaaatcccg atcggccatt ttttgcactg tctcatcgca tacccctttc catcgttcaa
    13441 gacactaaac gatctgcacc ggcttcttgc ccctcgcgga aagcttttcg gcatcgacaa
    13501 tctttaccga atcgcggcat gcctccgggg tggtcaccgc ctcgaccgga aggcccttca
    13561 atttgtccac gaaccagttg atctcgttct gatacgaatc cgtagaaacc ggcaactccg
    13621 gcacaaacgg cccgccatcc atcggataca ggcagaactc cggctgacgg ttggtggcca
    13681 tgaccagtga ggcccgctca aaggcgattt ggaaaccgcg ctcaaaccca aagctcttcg
    13741 ccatacccca tccgccatcc gccacaacgg atggcccgcc gacatcatac aacgtggaaa
    13801 tgtggatcat ggtgccatca ctgtcataat acgcggagct ggtcaccgcc gccggcatgc
    13861 cgaacaggaa gtgaaccata tcggtatcgt gaatgtgcag atccaatgcc accccgccac
    13921 tgcgcgactc gtcaaagaac cagctcttgc cgccgtcacg cgtccagccg ggcgcttggc
    13981 tgtaccgccc gaagcgcgcg gcgatcacgc ggccatactc accgctttcg atcaagcgct
    14041 tggcatacac atattcgccg cggaagcgca gacactggcc gatcatcaac tccgtgcctt
    14101 gcggcgcggc gcgccaggcc gcaagcaact cgtcgcacgc cgcggcatca agcgccatcg
    14161 gcttctcgca aagcacatgg atccccttgg acaacgccaa cgcggcggtc gagacatgca
    14221 gaaacgtcgg cagggtgatg tccaccgcat ccggccgggc ctgatccagc atggtcgcga
    14281 agtcgtcata caagacagcg tcgccataat cgcacgacat atcggcaccc tgaacgtttc
    14341 cggccgcggc ggcggactgc tcgaactgtc ggcgatcctg atcgcacaac gccaccacct
    14401 gcaccccggg cacttttttc cagcacccgt agtgcatccg acccatgaaa ccgaaacccg
    14461 caatcgctac tttgatcata ctgcctgctc tttcgttatc ccttatcgct caaacgcttc
    14521 gcgtcaaccg ttgaacagaa gacttctgcc tgtttggaca cgacacgcat ccgcatcagc
    14581 cctcaagcgt ccacggcgcg cggtacgcca cacccagcat cgcgtccgcc tcgggatcgt
    14641 tcacgaagcg ttcggcgcgg ggattccact tcaggtcctt cctgcccaga cgcaagccga
    14701 tgttggccag atgcgcgatg gtgatggtcc ggtgccccgt ctcgcagggg gagatcaccg
    14761 gcctgccggt caggatgccc tcgatgaagt tgcgctcgtg gttgctgctc acatagagct
    14821 tcacctcgcc gtcacgcggc ttctcgcgga tcagatcgaa cggcttggtc tccagcttgc
    14881 cgccgcaaat gtacaactca cggccgttct cgccgaaata gcgcaggaac cgtgacgggt
    14941 tctcgccgaa gtccgtggag gtgtgcacgc gcgtgccgtc ctcgtacacg atctcgaagc
    15001 tgaacctgga ggccacgttg tagaggctgt ccggcttgtc catctcgccc cggatgttct
    15061 cgatcgcgac cgggcccgac tcgtccatgc ccagcgacca ctgcaggatg tcgatgaagt
    15121 gcgcgccgtg gtcggtcacc atgccgccgc cgtactggaa gttgaagcgc cactgcatgg
    15181 gcaggtgcag cttggggatg taggggcttt gcggcgcggg ccccagccac aggtcgaagt
    15241 cgacgtacgc aggcggccgt gtcagcggcc gctcgctgag ctgctccgcg gtcttgcccc
    15301 aggccggcat gccgccggga agcccgagct ggatccgctg cagcttgccc aaccgccggt
    15361 tgcggatcaa ctcggccgct ctgcgttggt tcgggtcact ggagcgtccc tgcgccccgg
    15421 tctgaaagac cacgccgtgt tttttcaccg cctcgcacaa cgcgcggccc tccgccacgc
    15481 tgttcgccag cggcttctga ccgaagatgt gcttgccctt gcgcgcggcc atgatgccga
    15541 tcaacgcgtg ccaatggtcc ggcgtggcca cgcacaccgc gtccagatcc ttgtcctcca
    15601 gcatcgcgcg gaaatcggcg aacaccttgc acccgtcgta tccgggcttg ttgttctttt
    15661 tggcgtagaa gtcgttcacc tgccaccgcg ccggctcccg gccgccctga agctggccac
    15721 ggtatccgta gagcggcgcc tccgaaaccg gatcgcagac ggaaacgacc tgcgtgttat
    15781 cgtcatgcag gaagttgttg aggtttccgg tgatcccctg aaccccgcac ccgatcaagc
    15841 ccacattgac gcggccggag ggtgcgggac gccccgcttt ttgcccgaac gccgaagacg
    15901 gaataagatt cggaaacgcg cagaccgcgc ctgcggccgc cgcgaaacgg cgtcgtgaaa
    15961 cccgtgccaa gctcataccc tcaaccctta cctcccttga gtcagatccc cacgctgtgc
    16021 cgtgtacaat ggcgcatccg cctgcgcttt gtcaatccga tgattccggt aatagaggac
    16081 gaggtcggtg ccgaccatga ccagattgaa cagatagagc cagaccgcgc cgtcaacccg
    16141 ctcctgccaa aacagcttgt ggcacacccc gaaaatgtaa ccggcgaaaa caatcaccat
    16201 gaaaaaaagg cttttccccc tgttggtccg cgaccgccac gacttcagga tggaaaccgg
    16261 ccaagccgcg ccgaaacaaa tcaacatcat cgcttcaaaa accggaaaag acatacgctc
    16321 ctttcagtac tcgtacttgt tcacttaaat ctggtcattt tataaatccg ttttgccctt
    16381 tgcaactgac agggttcggg gaaccgtccc tacctccagc atacatctct tccgcctgtt
    16441 ttgacttgga ggcctcacgg cgccgtacgg acacatcgga agcctatcgg aaaataccgt
    16501 gtattatcca catgcgacgc cagatagcgg tccgataccg tgttattcga attgcatccg
    16561 ccgccgcggc gtcccatccc gttcggccat gtggcatttg cggcctcgcc ggcggttgtc
    16621 agcgtacccg accctccggc gagagcgtcg catgccgcca gtcattcgat cctgcgggtt
    16681 tcactttgac gaagacccaa gccgcgtccc agttccccgc gtcccgccag gcgttgttcc
    16741 aggcaagatc gaacgtcacc atcacgcact gacgcgcctc gtcccagtcg gtcagccgca
    16801 cgcgggagac ctgaatgtca tgcgcggaaa cctccgccac gaacaaaagg gataaacacg
    16861 ccaacccgaa taaccctcgt ttgttgactc gcatcggcca ccctttcatg cagacccggc
    16921 cccagaccac accggaacct tttttggtca tgacataact gtaccacaaa accgatcgca
    16981 gaaggttatg aataattgtc agacctgcca aagaatccaa acgccgcagg tcgaatgttg
    17041 cggatcgcaa gtcaccgttc acgaaaccgc gaacttggac gatggcgaat ggcatcatgt
    17101 ggctgttgcg attgacaaga cggccgttcg ctattatttt gacgctgttc cgtatgcgcc
    17161 gcaactgccg acaggagggg ggtactcctc aaggccggag agatcatgag gagcttggtg
    17221 tttttcggag tcgtcgcggc ggtggacaca gacagatggc acgggcatct tgcccgtgtt
    17281 tcatgggcgg gacgcccatg ccacgagggg ttttgcaaga gcctcctgag attaggaaaa
    17341 agacatggta ggagctttag ctgctgcggt agtgttgaaa ggggcgtatc cgctgttggt
    17401 tacgccctac acggaaaaag cggaactgga ttgcgaagtc ctgatccgag aggcggaata
    17461 tgtcgacgcg cagggcgtgg gcggcgtaat ctggcccacg gcaaacgaaa tcaacgttct
    17521 tgacgagttt ggcgagtacg agtcgggact gacggcgctt gccgatcgct gggcaaaggg
    17581 cgggatgaaa tcccggctca cggcggtctg ttgcggaacc aactccgccg cggcgctgaa
    17641 acgggttgag ctttgggacg ccatcgtgag caagcgcggc attt
//
LOCUS       DAOSLE010000052        17650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3421_length_17650_cov_7.937141, whole genome shotgun sequence.
ACCESSION   DAOSLE010000052 DAOSLE010000000
VERSION     DAOSLE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17650
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_17650_cov_7.937141"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK576_05200"
     CDS             complement(<1..658)
                     /locus_tag="PK576_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61023.1"
                     /translation="MNDLTVLYLQNQPLAPDALQTLTHGLSDTFRAFCNETFPSEEPI
                     DWPPLRVVPVTSTQTLKQALFAVGPASGPLTEDGKNCVLFLLDDAFRDPASDQTIPLH
                     SLTLDGIPLTLWLYTYFPPIPKIVLTTPGTERVRVPSRRWVLKPREVFLQIHAHLPRV
                     RHLFRSLWEPRFWHALRHYVMDEAGSSWHTPGHNAGHAFSRSLFLQGFRHEYGPMTFR
                     A"
     gene            1028..1258
                     /locus_tag="PK576_05205"
     CDS             1028..1258
                     /locus_tag="PK576_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61024.1"
                     /translation="MTAALRDRMHRGEGTVLAEGVPAVVTLPSVAAKTRCYALDERGA
                     RKGDVPVERVAGGCRVAIGPQYKTVWYELDIR"
     gene            1386..1835
                     /locus_tag="PK576_05210"
     CDS             1386..1835
                     /locus_tag="PK576_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOE61025.1"
                     /translation="MVAVLSGVALPLLIWWSGCLTDRIRPPSGSPSEVMVFEVTGYCD
                     CGICCNWERKWFGLGGAVVSSGPNKGQPKEIGVTASGTRTRHGTVAADTSVLPFGTIV
                     YVPGYGYGRVEDRGGAIKGAKLDLWYRSHAEAREWGRKTLRVRVWKP"
     gene            1887..3620
                     /locus_tag="PK576_05215"
     CDS             1887..3620
                     /locus_tag="PK576_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOE61026.1"
                     /translation="MNDENTAAGPDTGRHFLKQWIAEDVAAGRTGGQVVTRFPPEPNG
                     YLHIGHAKAICIDFGMAREFGGQCNLRMDDTNPSKESDEYVESIKRDIRWLGFDWGSG
                     FYSAADLFGQMYEIAENLIRSGHAYVCGLTQEEWKAYRGIPTAPGRESPTRNRSSEEN
                     LALFRRMRAGEFPDGSLCLRAKIDMASPNIHFRDPVLYRILHVPHYHAGDTWCIYPMY
                     DFAHPIEDAFEGVTHSMCTLEFEVHRPLYDWVIDRMDEMGLLVVRNGVKIRPQQREFA
                     RLNLTYTVMSKRKLLQLVEEKHVTGWDDPRMPTICGLRRRGYTPEAIRDFCERIGVSK
                     FESETDVALLEYCVRDDLNRRAVRRMAVLDPVKVVIDNLPEGHTAMVEVANNPEDPAA
                     GTRKIPFSREVYIERDDFMEVPAKKFFRMAPGQEVRLRAACLFTCTHVVKDDAGTITE
                     IHGTYDPDSTGGNAADGRKVKGTLHWVSVAHAVPLEVRLIDRLFTVPDPLGDPQRDFL
                     SFLNPDSCRTVTAYAEPALQEAEPGERFQFERLGYFCADTRDSRPGAPVFNRTVTLKD
                     SWARQTAGSKR"
     gene            3639..5588
                     /locus_tag="PK576_05220"
     CDS             3639..5588
                     /locus_tag="PK576_05220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOE61027.1"
                     /translation="MAQNVVEKILSAHRVSGELTPGGELAIRIDQTLTQDATGTMAFL
                     QFESMGLDRVKADLAVSYVDHNTIQVGFENADDHAYLKSVAQRYGAVFSRPGNGICHQ
                     LHLERFGKPGATLLGSDSHTPTGGGIGMIAIGAGGIDVAVAMGGGPFYLACPKVTRVW
                     LTGALKPGVSAKDVILKVLSVYGTQGNVGRMLEYGGPGVATLGVPARATLANMGAELG
                     VTTSVFPSDEVTRAFLKAQAREEDWCALEADPDACYESTLEIDLSTLEPLVACPHSPG
                     RIATVASLAGQRVNQVLIGSCTNSSYRDLKTVALMLRGRKTHPDVEVGLAAGSRQVVT
                     MLACEGLLAELIAAGVRILENACGFCIGNHMSPGTNAVSLRTSNRNFEGRSGTQSAQV
                     YLVSPETAAAAALTGVFTDPRSLETLPREVAEPSAFRIDDSMFLFRETAGRGVPGTEI
                     VRGPNIGKVPTGQPLPDRLDGMATIKVGDKITTDHIMPAGARLKYRSNVPQYARYVFE
                     HVSSSFYAEASAHRDAGLHNVIVAGESYGQGSSREHAAMCPMYLGVRALLAKSIERIH
                     FANLINFGIAPFLFDDPTAYDGISQGDRLVIDNLRTAIASDGRVTVRNVTRETTFTAT
                     AMLSTRQKNLLLNGGLLASTAGNAQ"
     gene            5634..7721
                     /locus_tag="PK576_05225"
     CDS             5634..7721
                     /locus_tag="PK576_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61028.1"
                     /translation="MSENEKAKPAADGQENDEIVLDLNFAPNWARTSPEDQIQRYRND
                     RYDDDGEGHDRFGGRDRRPARFDGGDRDRRRPDRRPPPRRDRDERRGDFRGGGGMPRP
                     ASRGAVADLRGATSAAAPFEPRGPRPERRGPRTFRPVQPALPVDIRVLPEQKALGAVI
                     RRIQTSRRAFPLRDIAWLFLNNPNSCLVRIEPLKDQPVPLFQCKVCGMPALAEEDIRT
                     HLLNRHMEDFFDVEEVPCEPPTGVFVCVARCGMSGVLLGPPNHHSFNAKVQEMLRTRY
                     ANLSEEAYRARIEMVRDTEVVETWRQQCTRKKIYRRKGATPTVVEPVESATEDGDTET
                     PPEPEPKAPAMERETAELVFLREILPAQIASVRHLLCTASVAMQTSSRPLYYALKDIL
                     TRERRFPASLFFALRGAFRHRNLHLFRVNDAKGQDFVMFKPAVSLDPTHAVQALRDVL
                     TFVNEHPACTKAELATALAADDEAKLKETLVQLAWLVEKGHVIQFYNDVLSGPLEYPA
                     FRFLPGEKTAGGRQDGPRRPPSGTPASGDSAKDAPADAAAAVSDSTEETASAPAVEKT
                     PASGDSAKDAPADAAAAVPDSTEETASAPAVEKTPASGDSAKDAPADAAAAVPDSTEE
                     TVSAPAVEKTPASGDSAKDAPADAAAAVSDSTEETVSAPAVEKTPASAEESVVPIAEA
                     STAADEPRTAPEA"
     gene            complement(8081..8707)
                     /gene="purN"
                     /locus_tag="PK576_05230"
     CDS             complement(8081..8707)
                     /gene="purN"
                     /locus_tag="PK576_05230"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOE61029.1"
                     /translation="MLTPTLPLRIGVLGSGSGTNMQSVLDAIESGTLNAELRLVLSDV
                     QDAKILDRAARHKVPNAWLDCAPFKTKLDGEAEQRCIALLQAHGVDTVILAGFMRIVK
                     PGLLNAFPQRVLNIHPALLPAFPGLEGWKQALEYGVKLAGCTVHFVDAGTDTGPIILQ
                     RAVPVLTDDTPETLHARIQTQEHLAYPEALRLLAAGRLRINGRRVEVS"
     gene            8852..9400
                     /locus_tag="PK576_05235"
     CDS             8852..9400
                     /locus_tag="PK576_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOE61030.1"
                     /translation="MKCELCHKSDAETVLRKQVGDEVHEWFVCQACAQAAAKPPAPDR
                     NTRQTGEGGMPEASVLPLFGMIVDAAFEIADKQAMLTEPSCPVCGIRRSEYRKRSRLG
                     CPACYEAFADELDAALQELHRATRHTGRAPRNAEAACCRQELERALDEAVREQRYEEA
                     IALRDRLRALDGGQDAAPGGVS"
     gene            9397..10455
                     /locus_tag="PK576_05240"
     CDS             9397..10455
                     /locus_tag="PK576_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HOE61031.1"
                     /translation="MKKLTPKRKTSANPVDGARAEEVALVCRVRLARNLEGERFPDWA
                     TEHDHARVFERVRSAFGAAAPDLALRPLSDFNVRYLEMLCEQNLISRDLLRRTSGGGI
                     AFEPPSAVCVMVNEEDHLRIQGFADGFDLNRAWQRATRMDALLEPHLHYAWSLRLGYL
                     TACPSNVGTGLRAGVMVHLLGLRLLGEIEAVMRGLERMRLLVRGIGGEGSEASGQIYQ
                     ISNMDTLGVEEEAIVGRVTRIVAEVIRQELNARERLLRESPLLLTDCLVRALTVLQNA
                     RLMSAAEALELLSAVRLGAVLGRFSKLKPVMVNQLILLMQPGHMQSGLQADLNPDQRD
                     EMRAAFLNQKLANVKWKG"
     gene            10461..13127
                     /locus_tag="PK576_05245"
     CDS             10461..13127
                     /locus_tag="PK576_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOE61032.1"
                     /translation="MDGFGNFTARAQRALQLAAREADRFNHPYIGTEHLLLGLIALGE
                     GVAVEVLEELDVSLQDVRIAVERMVGFGGETKTKGELPHTPRTKKVLHLAMAEAHAMR
                     QPQVGTEHLLLALLREGEGVAAQVLQSLHVRLEAVIEQIRLSMEAGEGDEEDGVFGPG
                     EGSGREEREARAAPDGKPGEGAGSKKERSRTPALNAFGRDLTELAKKGELDPMVGRAN
                     ELERLIQILCRRSKNNAALLGEAGVGKTAVVEGLAQAIALGDVPERMRDKRVVALDMA
                     LLVAGTKYRGQFEERIKAVVDEVCREKNIVLFLDELHTIVGAGGAEGAMDAANIIKPA
                     LARGELQCIGATTLNEYRRHIEKDAALERRFQTVRVDEPTVEETVEILKGLAPRYAAH
                     HNVTYEEDSLIAAAKLTARYQPGRQLPDKAIDAIDETGARVRMRVSARPPDLREHEQR
                     IREMGQKKDIAIREQRFEEAAALRDEERKARQELDAIVAAWKTEHAEKTLTVTADDVT
                     ETVARIAGVPIKRMSQSELSRLLHIENDLEEAVVGQTPAVQAIARALRRSRADLKDPR
                     RPIGSFIFLGPTGVGKTLLAKTLAEKMFGDDKALIQIDMSEYMEKFTVSRLIGSPPGY
                     VGHEEGGQLTERVRRRPYSVVLFDEVEKAHPDVMHMLLQILEEGRLTDSLGRQVDFRN
                     TVVILTSNLGFDYAKQGQGLGFGRESAADDYERLRERMIDSAKLVFKPELLNRFDEII
                     VFRKLDRQDVVRILDIELAGVRKRLETKGICLEITEEAAAFLVDKGFDPALGARPLRR
                     TVERHLEDPLAEELLRGVLTAGTVETTLKEDGTGLHFRMQDELPLRTPDKKTASPPRN
                     RKKKKTESATTVDAGTRPAPKPRKTRKRGKAE"
     gene            complement(13174..13302)
                     /locus_tag="PK576_05250"
     CDS             complement(13174..13302)
                     /locus_tag="PK576_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61033.1"
                     /translation="MVIFRTGTVAQPSITNGTAFVDDLRVTVFEPVWPPTTLLKLF"
     gene            complement(13296..13670)
                     /locus_tag="PK576_05255"
     CDS             complement(13296..13670)
                     /locus_tag="PK576_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61034.1"
                     /translation="MRPRCQWAGIAVALILIGGRAESKTVNVFFAGGQSNAKAVWATA
                     IADGLKAGYGSSLVMAHTNHSGEGLANWFTDTSKINYSNDLFHLNGTSVLQTRMAEIA
                     AAGDEPVFRGDVRPVSAEFRLW"
     gene            13850..14872
                     /locus_tag="PK576_05260"
     CDS             13850..14872
                     /locus_tag="PK576_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61035.1"
                     /translation="MERNGTRRGFLRMGACGASVWALSGGVRQAEGASTDKSFGHGSF
                     ALDGNRIRVFSPAVREPVKVWVAGDTHLHRDDARGEPFREYSSRMAGGYNRVRHVESG
                     ELTTPEASFEETLRRAKEARVDLLALVGDVFSFPSEAAIEWAQAKLDASGVPYLYVAG
                     NHDWHYEGLPGSSMALRSEWTEKRLKPLYQGQNPLMAVREIRGIRFVAIDDSTYEILP
                     EQRAFFREQVADNTPLVLLMHIPLYAPGRPVGFGCGHPDWGAAQDRHWKIERRPQWPA
                     AGHTAVTRAFRNEVFSAPGILGVFAGHTHVQSLDVVNGVPQVVTAANAGCGMVRAEFL
                     PAHNGH"
     gene            14939..15985
                     /locus_tag="PK576_05265"
     CDS             14939..15985
                     /locus_tag="PK576_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61036.1"
                     /translation="MAKRKPVQKRVAIVGFGFMGRMHYGQWKKIRNAKIVALCDQNQA
                     QFTAPVQGGNVAGADTSMDYGDAVIYDDFDRMLKEARPDVISLTLPTPLHVPFTVKAL
                     KAGVSVLCEKPMALNAVDCGVMLRAAKQAPKGTRLMIAQCLRFWPSYVYLKDLIDSGK
                     YGKVIAASFYRFSAPPGWQKGVNWFMDESKSGGVALDLHIHDTDIVQFLFGMPRAVTS
                     RANFAKDGAMRYISTLYDVGGAAVTAEASWAMAPTLGFEAGYVVTFEKAVVVLDDKRG
                     KPLCVYPAKGKAFVPKLPKGDGYEYEIRWFMDLLCGRKVACVTTPEQSRDSVRIVDAE
                     KRSARTGRTVRLAK"
     gene            16044..16859
                     /locus_tag="PK576_05270"
     CDS             16044..16859
                     /locus_tag="PK576_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE61037.1"
                     /translation="MLKCGKWPVAVCSWSFRKSVEEIGSVMTALGLEHINLAIAPALA
                     PDGEAYLDAVRKQGWTVTAGMMNYDYEDYSTLDTIKVTGGIAPDAYWPASGEAFARAA
                     QITAALGSQYIMMHVGFLDHTDNAYAKKFYDRVRALGDSAGEAGVTLLMETGQETAEE
                     LQRFVETLNHPNVLLNFDPANLILYNKDEPLPALRRLAPWIRHIHIKDAIRTTVPGTW
                     GSEVVWGEGQVNAFAFLNVLEEIGYEGAVAIEREAGEQRVRDIATAVRRLVAY"
     gene            complement(16862..>17650)
                     /locus_tag="PK576_05275"
     CDS             complement(16862..>17650)
                     /locus_tag="PK576_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOE61038.1"
                     /translation="VVHQASTLTVTFPDGSRYDAVLVGIAPDYDLAVLRINAPPEKLV
                     PVTVATSRALQVGQQVYAIGNPFGLDTTLSAGLVSALGRTITSMTDRKIYDVIQTDTA
                     INPGNSGGPLLSSSGRLVGVNTAILSPSGAYAGIGFAVPSDTVSRVVPQLIEKGRVTR
                     AGLGVYLLPDHVTARAGIAGVALYAVAERTPAARAGLEGIGVSRLGDLIFGDVIQAVD
                     GIPVGTIEELQAVLDPRRPGEQVRLTVARNGKRRDVTLTLIEEE"
BASE COUNT         3419 a         4846 c         6072 g         3313 t
ORIGIN      
        1 cggcgcggaa agtcatcggg ccgtactcgt ggcggaaccc ctgcaggaaa agcgagcggc
       61 taaaggcgtg gccggcgttg tggccgggcg tgtgccaaga ggagccggcc tcgtccatca
      121 cataatggcg cagggcatgc cagaagcgcg gttcccaaag ggaacggaag aggtgacgga
      181 cacgcggcag atgggcgtgg atttggagaa acacctcgcg gggtttgagc acccatcgcc
      241 gcgaggggac ccgcacacgt tctgtgcctg gcgtggtcag gacgattttc gggatcggcg
      301 gaaagtaggt atagagccag agcgtgaggg ggataccatc tagcgtcaaa ctgtggagcg
      361 gaatcgtctg gtcggaggct ggatcgcgga acgcgtcgtc cagcaggaag agaacgcagt
      421 ttttaccgtc ctcagtgagc ggcccgctcg ccggacccac cgcgaagagc gcttgtttca
      481 gggtctgcgt gctggtgacg ggcacgaccc gcagtggtgg ccagtcgatc ggttcctcgg
      541 aggggaaggt ctcgttgcag aaggcacgga acgtgtccga aagtccgtgc gtgagcgttt
      601 ggagcgcgtc cggggcaaga ggctggttct ggagatagag aaccgtcagg tcattcataa
      661 cttctaagac ggacggggtg ccgcgctgtc gagacgctcc gcctgttctg gtttcctttc
      721 ccaatctagg tgacgagaag aggataaaat aagaacggac ggctgacaac agcaaactcc
      781 ggcatgccgt agatggggcg tcgaaacgcg agttgggccg ggtttgcgca agacagagat
      841 catttttacg caaaaggggt ggaggaaaac ggcggggttc gatatgatgt aaacatccgt
      901 aatgttgggg atgccccgtg cggttatttg gacgggacgc tgacgctatg gcctgacacg
      961 tgtgatgcgg tcaacgggct tgtggagaac ggattgaggc agcatgatgt gaaaaggggg
     1021 tagatccatg acggcagcgc ttcgcgaccg catgcaccgg ggtgagggga cggtgctggc
     1081 tgaaggtgtg cccgctgtcg tgacgctgcc ctctgttgca gcgaaaacac gctgttacgc
     1141 gctcgacgag cgcggggctc gcaagggtga tgttccggtc gaaagggttg cgggcggttg
     1201 ccgtgtcgcg attgggccgc agtacaaaac cgtgtggtac gaactcgata ttcgttaagt
     1261 cggaaaaatg aaaggactcg ggcgcaagag agcgttcagc attgtgggga cgggccgcat
     1321 ttgatacagt accccttatg tccaaaccgt gcacagggga ctatccaaac cgatggcgat
     1381 ggcgtgtggt tgccgtactt tccggcgtgg cgctgccgtt gttgatatgg tggtcggggt
     1441 gtctcacgga ccggatccgt cctcccagcg gaagcccgtc tgaagtgatg gtttttgagg
     1501 tgaccgggta ctgcgactgc ggcatctgtt gcaattggga gcggaagtgg ttcggtttgg
     1561 gaggtgcggt ggtcagcagc ggaccgaaca aagggcagcc caaggagatc ggggtgacgg
     1621 ccagcgggac gcgtacccgg cacgggactg tggcggcgga tacttccgtg ctgccgttcg
     1681 gaaccatcgt gtatgttccg ggctacggtt acggacgcgt tgaggaccgc ggaggcgcga
     1741 tcaaaggtgc caagctggat ttgtggtaca gatcgcatgc ggaggctcgg gagtggggtc
     1801 gcaagaccct ccgtgtgagg gtatggaaac cgtaaaaaaa cgggcttccc ggtgaaggga
     1861 atgcttaacc aaagaccaga aacgatatga atgacgagaa cacggcggcg gggccggata
     1921 ccggcaggca ctttttgaaa cagtggattg cggaggatgt ggcagccgga cggacggggg
     1981 ggcaggtggt gacgcggttt ccgccggaac cgaatggata ccttcacatc gggcacgcca
     2041 aggcgatctg catcgacttc ggtatggcgc gggagttcgg gggacagtgc aacctgcgca
     2101 tggacgacac caacccctcc aaggagtcgg acgaatacgt cgagagcatc aagcgggaca
     2161 tccgttggct gggttttgat tggggaagcg ggttctacag cgcggccgat ctgttcggcc
     2221 agatgtacga gatcgccgag aacctgatcc gtagcggtca cgcttatgtc tgcgggctga
     2281 cgcaggagga gtggaaggcc tatcgcggca tcccgacggc tccgggccgc gagagcccga
     2341 cccgcaaccg ttcgtctgaa gagaatctcg cgttgttccg acgcatgcgg gcgggagaat
     2401 ttcccgacgg gtcgctctgc ctgcgcgcca agatcgacat ggcgtcgccc aacattcatt
     2461 tccgcgatcc ggtgctttac cgcattctgc atgtgccgca ctatcatgcc ggcgacacgt
     2521 ggtgcatcta tccgatgtac gatttcgcgc atcccatcga ggatgccttc gaaggggtga
     2581 cccattcgat gtgtacactc gaattcgaag tgcaccgtcc gctgtatgac tgggtgattg
     2641 accggatgga cgaaatgggg ttgctggtgg tgcgcaacgg ggtcaagatc cgtccgcagc
     2701 agcgggagtt cgcgcggctg aacttgacct acaccgtgat gagtaaaagg aagctgctgc
     2761 agttggtgga ggagaagcac gtcacgggtt gggatgatcc ccgcatgccc acaatctgcg
     2821 gcttacggcg ccggggctat acccccgagg caatccgtga tttctgcgag cgcatcggcg
     2881 tgtcgaaatt cgagagcgag acggatgtgg cattgctgga atactgtgtg cgcgatgatc
     2941 tcaaccggcg cgcggtccgg cgtatggcgg tgctcgatcc cgtgaaggtg gtgatcgaca
     3001 atctgccgga ggggcacaca gcgatggtgg aggtggcgaa caatcctgag gatcccgccg
     3061 ctggcacgcg caaaatcccc ttcagccgcg aggtgtacat cgagcgcgac gattttatgg
     3121 aggtgcctgc gaaaaagttt ttccgtatgg ctcccggtca ggaggtgcgc ctgcgcgccg
     3181 cctgcctgtt tacctgcacg catgtggtga aggacgatgc cgggacgatc accgaaatcc
     3241 acgggaccta cgatcctgac tcgacggggg gcaacgccgc cgacggccgg aaagtcaagg
     3301 gtaccctcca ttgggtctcc gtcgcgcacg ctgtgccgct ggaggtgcgc ttgattgacc
     3361 ggctctttac ggtgccggat ccgctgggtg atccgcagcg tgattttctc tcgttcctca
     3421 atcctgactc ctgccgcacg gtgaccgcat atgccgaacc ggcgttacag gaggcggaac
     3481 cgggagagcg ctttcagttc gagcggctcg gatatttctg tgcggacaca cgcgactcgc
     3541 gccccggcgc gccggttttc aaccggaccg tgacgctcaa agactcatgg gcccggcaga
     3601 cagccggatc gaaacgctaa cgggagaaag ggtttttcat ggcgcaaaat gtggtggaga
     3661 agatcttgtc cgcacaccgg gtgtccgggg aactgacacc gggcggggaa ctggcgattc
     3721 ggattgatca gaccctgacg caagatgcga ccggcaccat ggcgttcctg caattcgaaa
     3781 gcatggggct tgaccgggtg aaagccgatc tggcggtcag ttatgtggac cacaacacga
     3841 ttcaggtggg ttttgagaat gcggacgatc acgcttacct caagagtgtg gcgcaacgat
     3901 acggcgcggt tttctcgcgg cccggtaacg ggatctgcca ccagttgcat ctcgaacgtt
     3961 tcgggaagcc gggcgcgacg ttgctgggca gcgattcgca cacgcccacg ggcggcggga
     4021 tcgggatgat cgcgattggc gcgggcggaa tcgatgttgc ggtggcgatg ggcggcggac
     4081 cgttttatct ggcctgcccg aaggtgacgc gggtgtggtt gaccggcgca ctcaagccgg
     4141 gggtctcggc caaagacgtg atcttgaagg ttctgtcggt gtacggcaca caaggcaatg
     4201 tggggcgcat gttggagtat ggcgggccgg gtgtggccac gctcggcgtg ccggcgcgcg
     4261 cgacccttgc caacatgggg gcggaattgg gcgtgaccac ctcggtattc ccaagcgacg
     4321 aggtgacgcg ggcttttctg aaggcacagg cgcgcgagga ggattggtgc gcgttggagg
     4381 cagacccgga tgcgtgctac gagagcacgc tggagatcga tctgagcaca ctggaacctc
     4441 tggttgcgtg tccgcattcg ccagggcgca tcgcgaccgt tgcgtcgctt gccggccagc
     4501 gggtcaatca ggttctgatc ggttcatgta ccaactcctc ctatcgggat ttgaagaccg
     4561 tggccctgat gttgcgggga cgcaagacgc atcctgatgt cgaggtcgga ttggccgcag
     4621 gatcacggca ggtggtgacg atgctggcat gcgaggggtt gctggccgag ttgatcgccg
     4681 ctggggtgcg tattctggag aacgcctgcg gcttctgcat cggaaaccac atgtcgccgg
     4741 gcacgaatgc cgtcagtctc cgtaccagca accgcaactt tgagggacgt agcgggacac
     4801 agtcggcgca ggtgtatttg gtgagccctg agactgcggc cgcagccgcg ttgaccggcg
     4861 tgttcacaga tccgcgaagt ctcgaaaccc tgccgcgcga ggtggcggaa ccgtcggcgt
     4921 tccggatcga cgacagcatg ttcctcttcc gcgaaaccgc aggccgaggg gtcccgggaa
     4981 ccgaaatcgt gcgcggtccc aatatcggaa aggtgccgac cggtcagccg ctgcctgacc
     5041 ggttagatgg aatggcgacg atcaaggtgg gcgacaagat cacgaccgac cacatcatgc
     5101 ctgcgggggc gcgtctgaaa taccgctcca atgtgccgca gtacgcccgg tacgtttttg
     5161 aacacgtcag ttcgtccttt tacgctgagg ccagcgccca tcgcgatgcg gggcttcaca
     5221 acgtcatcgt ggcgggggag agttacggtc agggatcgag tcgcgagcat gcggccatgt
     5281 gcccgatgta tctcggggtg cgggctctgc tggccaaaag tatcgagcgt atccattttg
     5341 cgaatctgat caatttcggc atcgcaccgt ttctttttga tgatccgacg gcctacgacg
     5401 gaatttcgca gggtgaccgc ttggtgatcg acaatctgcg cacggccatt gcctccgacg
     5461 gacgggtgac ggtgcgcaat gtaacgcggg agaccacgtt caccgctaca gcgatgttga
     5521 gtacacgtca aaagaatctg ctgctgaacg gcggactgct cgcttccacg gcgggcaacg
     5581 cgcaataata tccattgacc attcggtcag acaaaaggta acatccacgc atcatgagcg
     5641 agaacgaaaa ggcgaagcct gccgcagacg gacaggaaaa cgacgagatt gtgttggatc
     5701 tgaatttcgc gccgaactgg gcgcgtacct ctccggagga tcaaatccag cgctatcgga
     5761 acgaccggta tgacgatgac ggggagggcc acgatcgttt cggcgggcgc gaccgtcgtc
     5821 cggcgcgctt tgacggcggc gatcgggatc gacgccgtcc cgatcgtcgt cctcccccgc
     5881 gccgtgatcg tgatgagagg cgtggggact tccggggcgg cggcggcatg ccgcggcccg
     5941 cgtcgcgtgg tgcggtggcg gatctgcgcg gtgcgacgtc cgctgcggct cccttcgagc
     6001 cgagagggcc gcggcccgag cggcgcggtc cgcgcacgtt tcggcccgta cagcccgcct
     6061 tgccggtcga tatccgcgtg ttgccggagc agaaggctct gggggccgtg atccggcgca
     6121 ttcagacctc gcgccgagcg ttcccgctgc gtgatatcgc ttggctgttt ctcaacaacc
     6181 ccaactcctg tctggtccgc atcgaaccgc tcaaggatca gccggtgccg ttattccagt
     6241 gtaaggtgtg cggcatgccc gcgctggcgg aagaggatat ccggacgcat ctgctcaacc
     6301 gccacatgga ggattttttc gatgtggagg aggtgccgtg tgagccgccg accggtgtat
     6361 tcgtatgcgt ggcgcgatgc ggcatgagcg gcgtattgct cggccccccg aaccatcaca
     6421 gtttcaatgc caaggtgcag gagatgttgc ggacgcggta tgcaaacctg tcggaggaag
     6481 cctatcgtgc gcgcatcgag atggtgcgtg ataccgaggt cgtcgaaaca tggcggcagc
     6541 agtgcacccg taaaaaaatc tatcgccgta aaggtgcgac accgacggtt gtcgagcctg
     6601 tcgaatccgc gacagaagac ggggatacgg agacaccgcc cgaacccgag cccaaggcgc
     6661 cggccatgga gcgtgaaacc gcggagttgg ttttcctgcg tgagattttg cccgcgcaga
     6721 tcgcgtcggt ccgtcatctg ctctgtaccg cgtcggtcgc gatgcagaca tcgagccgac
     6781 cgctctatta cgctctgaag gacatcttaa cgcgcgaacg ccgttttccg gcttcactct
     6841 ttttcgcgtt gcgcggcgcc ttccggcatc gtaatcttca cttgttccgc gtcaatgacg
     6901 ccaagggaca agattttgtg atgttcaaac cggcggtgtc gctagatcct acgcacgcgg
     6961 ttcaggcgtt gcgcgatgtg ctgacctttg tcaacgagca tcccgcttgt accaaggccg
     7021 aactcgccac tgcactggca gccgacgatg aggcgaaact caaagagacg ctggtgcaac
     7081 tcgcgtggct ggtggagaaa gggcatgtga ttcagttcta caacgacgtg ctgagcggtc
     7141 ctcttgaata tcccgccttt cgcttcctgc cgggagaaaa gacggctggc gggcggcaag
     7201 atggcccacg gcgtccgcct tcgggcacac ccgcatccgg cgactccgcc aaggacgcgc
     7261 ctgccgatgc tgcggcggcg gtttcggaca gcacggagga gacggcgtcc gctcccgccg
     7321 ttgagaagac gcccgcgtcc ggcgattccg ccaaggacgc gcctgccgat gctgcggcgg
     7381 cggttccgga tagtacagag gagacggcgt ccgctcccgc cgttgagaag acgcccgcgt
     7441 ccggcgactc cgccaaggac gcgcctgccg atgctgcggc ggcggttccg gacagcacgg
     7501 aggagacggt gtccgctccc gccgttgaga agacgcccgc gtccggcgat tccgccaagg
     7561 acgcgcctgc cgatgctgcg gcggcggttt cggacagcac ggaggagacg gtgtccgctc
     7621 ccgcagttga gaagacgccc gcgtctgcgg aagagtctgt cgttcccatc gcggaagctt
     7681 cgacagcggc agatgaaccg cgcacggctc cagaagcgta agacttgcct tacttatctt
     7741 atgtgggtgt gggtataaac cggtttcagg ttcacattct tcacgttgtc cccttgtcca
     7801 cttctcatga gaagtggaca aactgaccat ccggaacttc gctccggatg aacagtgaac
     7861 ctgggagggg gataagcgga acgtggatac cgacctgcgg cccgcggcat tcggcccgcg
     7921 acgctcgacc cgcgatgctc ggcccgcgac gctcgacccg cgacgctcgg cccgcgacgc
     7981 tcggcccgcg atgctcgacc cgcggcattc ggcccgcgac gctcgacccg cgatgctcgg
     8041 cccgcgacgc tcgacccgcg atgttcgatc tgctcgtcct tcaggagacc tcgacgcgcc
     8101 gtccgttgat gcggaggcgt ccggcggcga gcaggcgtag cgcttccgga tatgcgagat
     8161 gctcttgcgt ctggatccgg gcgtgcaggg tttcaggcgt gtcatccgta agaaccggaa
     8221 cggctcgttg gaggataatc gggccggtgt ccgttcccgc atccacaaag tggacagtgc
     8281 accctgccag cttgacaccg tactccaaag cctgcttcca tccctccaac cccggaaatg
     8341 ccggaagcaa tgcgggatgg atgttcagca cccgttgggg gaaggcgttc aacaatcccg
     8401 gtttcacgat gcgcataaac cccgcgagga tcacggtgtc aacgccatgc gcttgaagca
     8461 gcgcgatgca gcgttgttcc gcctcaccgt ccagtttggt cttgaagggg gcgcaatcga
     8521 gccaggcatt cgggaccttg tgccgtgcgg cgcggtccag gatttttgcg tcctgcacat
     8581 ccgacaggac caaacgcaac tcggcattca gggtgccgga ttcgatcgca tcgagcacgg
     8641 actgcatgtt ggttccggaa ccggaaccca gaacgccgat ccgtaacggt agggtagggg
     8701 taagcatgtc ggtagcgtaa tacaatccgt ccggcgtggc aaccgggaag tcgggcgtct
     8761 ttcgattgca ctgaaaacgc gcgcaaggag gccgtgccgg tttgcggtgc gtccctcttt
     8821 ctgctattct ttttcttttt caggaaaacg gatgaaatgc gaattgtgtc ataagtcgga
     8881 tgcggaaacc gtcctccgga agcaagtggg ggatgaggtc cacgaatggt ttgtttgtca
     8941 ggcgtgcgcg caggcggctg cgaaaccgcc cgcgccggat cggaacaccc gacagaccgg
     9001 agaagggggt atgccggagg cttcggttct gccgttgttc gggatgattg tggatgccgc
     9061 gtttgaaatc gcggacaaac aggcgatgct cacggaaccg agttgtccgg tgtgcggcat
     9121 acggcggagc gagtaccgca agcgctcacg tctggggtgc ccggcttgct atgaggcttt
     9181 tgccgatgag ttggatgccg cgctgcagga acttcaccgc gcgacgaggc atacggggag
     9241 agccccgagg aatgccgagg cagcctgttg ccgtcaagaa ctggagcgtg cgttggacga
     9301 ggccgtgcgg gagcagcggt atgaggaggc gatcgctctg cgcgaccggt tgcgcgcgct
     9361 ggacggggga caggacgcgg caccgggagg tgtgtcatga aaaagctgac accgaaacgg
     9421 aaaacatccg cgaatccagt agacggggcc cgagccgagg aagtcgcgct ggtgtgccgt
     9481 gtgcggctgg cgcggaatct ggagggcgag cgctttccgg attgggccac ggagcatgat
     9541 cacgcgcgcg tgttcgagcg tgtacgttct gcgttcgggg cggcggcacc ggatctggcg
     9601 ctccggccgc tctcggattt caacgtgcgg tatctggaaa tgctgtgcga gcaaaacctg
     9661 atcagccgcg atctgctcag acggacttcg ggaggcggaa tcgccttcga gcccccaagc
     9721 gcggtctgcg tcatggtgaa cgaggaggat catctgcgga tacaaggttt cgccgacggt
     9781 tttgacctga accgtgcgtg gcagcgggcg acccgcatgg atgcgttgct cgaaccccat
     9841 ctgcattatg cgtggtcgct gcggctggga tatctgacgg cttgtcccag taatgtgggc
     9901 accggattac gcgcgggcgt gatggtccac ctgctcgggt tgcgtttgct gggcgagatc
     9961 gaggcggtga tgcgcggact cgagcgcatg cggcttctgg tgcgcggaat cggcggagaa
    10021 ggctccgaag cctctggcca gatctatcag atttccaaca tggatacgct tggtgtggag
    10081 gaggaggcga tcgtcgggcg cgtcacgcgg attgtcgccg aggtgatccg tcaggagttg
    10141 aatgcgcgcg agcgtctgtt gcgggaatcg ccgctgttgc tgaccgattg tctggtacgt
    10201 gcgctcaccg tgctgcagaa cgcgcggctg atgtccgcag cggaggcgct ggagttgttg
    10261 tcggcagtac ggttgggtgc ggtgctgggg cggttttcca agctcaagcc ggttatggtg
    10321 aatcagttga tcctgctgat gcagccgggg catatgcaga gcgggctgca ggcggatctg
    10381 aatccggatc agcgcgatga gatgcgggcc gccttcctga atcagaagct ggccaacgtg
    10441 aagtggaaag ggtgaggggt atggacggat tcggaaattt cacggcgcgc gcgcagcgtg
    10501 cgcttcagtt ggcggcccgc gaggcggacc ggttcaatca tccgtacatc gggaccgagc
    10561 acctcctgct gggactcatc gctttgggcg agggcgtggc ggtcgaggtg ttggaagagt
    10621 tggatgtttc gttgcaggat gtgcgtatcg cggtggagcg gatggtcggg tttggtggcg
    10681 agaccaagac caagggcgag ttgccgcata cgccgcgcac caagaaagtg ctgcatttgg
    10741 cgatggccga ggcgcatgcc atgcgtcagc cgcaggtggg aacggaacat ctgcttctgg
    10801 cactgctgcg tgaaggtgag ggcgtggccg cacaggtgct ccaatcgctc catgtccggc
    10861 tggaggcggt gatcgaacag atccgtctca gcatggaggc gggggaagga gacgaggagg
    10921 atggcgtgtt cggacccggt gagggttccg gcagggagga gcgtgaggcc agggccgcgc
    10981 cggacgggaa accgggggaa ggcgcgggca gtaaaaaaga gcgaagccgg acccccgcac
    11041 tgaatgcgtt cggacgcgat ctgaccgagc tggccaaaaa aggagaactc gatccgatgg
    11101 tggggcgcgc gaatgagttg gagcgtctga ttcagatcct ttgccgccgc tccaaaaaca
    11161 atgcggctct gctgggcgaa gccggggtcg gtaaaacggc cgtggtggag gggttggcgc
    11221 aggcgatcgc gttgggtgac gtgccggagc gcatgcgcga caagcgtgtg gtcgcgctgg
    11281 atatggcgct gctggtggcg ggcaccaagt accgcggcca gtttgaggag cggatcaaag
    11341 cggtggttga tgaggtctgc cgcgaaaaaa acatcgtgct gtttctcgac gagctacaca
    11401 ccattgtcgg tgcgggcggt gccgagggtg cgatggacgc ggccaacatc atcaagcccg
    11461 cgctggcgcg tggcgagttg cagtgcatcg gtgccacgac gctcaacgag tatcgacgcc
    11521 acattgaaaa ggatgccgca ctggagcgcc gctttcaaac cgtgcgtgtg gacgagccca
    11581 ccgtggagga gacggtcgaa attctaaagg gtttggcacc gcgttacgcc gcgcatcaca
    11641 acgtcaccta cgaagaggat tccctgatcg cggccgcgaa gctgacggcg cgctatcagc
    11701 ccggacggca acttccggac aaggcgatcg acgcgattga tgagacgggc gcgcgggtgc
    11761 ggatgcgggt ttcggcgcgc ccgccggatt tgcgcgagca tgaacaacgg attcgcgaga
    11821 tggggcagaa aaaggatatc gcgatccgcg agcagcgctt cgaggaagct gcggcgctac
    11881 gtgatgagga gcgcaaggcc cgtcaggaac tcgatgcaat cgtggcggca tggaagaccg
    11941 agcacgcgga aaagacgctg acggtgacgg cggacgatgt cacggaaacg gtggcgcgga
    12001 tcgcgggcgt gccgatcaaa cgcatgagcc agtcggaact ctcgcgcctg ctgcacatcg
    12061 aaaacgatct ggaagaggcg gtggtggggc agacccctgc ggtacaggcg attgcccggg
    12121 cactgcgtcg ctcgcgggcc gatttgaaag atccgcggcg tccgatcggc tccttcattt
    12181 ttctggggcc gaccggtgtg ggcaaaaccc tgctggccaa gactctggcc gaaaagatgt
    12241 ttggtgacga caaagccctg attcagatcg acatgtcgga gtacatggag aaattcacgg
    12301 tctcgcggct tatcggctcg ccgcccggtt atgtggggca cgaagagggg gggcagctta
    12361 ccgaacgtgt gcgcaggcga ccctatagtg tcgtgctgtt cgacgaggtc gagaaggcgc
    12421 atccggatgt gatgcacatg ctgcttcaaa ttctggagga gggccgcctc acggacagtc
    12481 tgggacgtca ggtggatttc cgcaatacgg tggtgatcct gacctcgaac ctcgggttcg
    12541 attacgcgaa gcagggtcag ggactgggct tcggacgcga gagcgcggcg gatgattatg
    12601 agcgtttacg cgaacgaatg atcgactccg cgaaactcgt gttcaaaccg gaattgctga
    12661 accgatttga tgagatcatc gttttccgga aactggatcg gcaggatgtg gtccggattc
    12721 tggacatcga actggccggg gtccggaagc gtctcgaaac aaaggggatt tgtctggaga
    12781 tcacggaaga agcggccgca tttctggtcg acaagggatt cgatccggca ctcggcgcgc
    12841 gtccgttgcg gcgtacggtg gagcggcatc tggaggatcc gctcgcagag gaactgttgc
    12901 gcggcgtact gactgcgggc acggtcgaga cgacattaaa agaggacggg acaggattgc
    12961 acttccgcat gcaggacgaa ttgccgttgc gtacaccgga taagaaaacg gcctcgccgc
    13021 cccgtaaccg taagaagaaa aaaacggaga gcgcgacaac ggtggacgcc ggaacccggc
    13081 ccgccccgaa gccgagaaag acgcgaaaac gcggcaaggc ggagtgattc agaggcggtc
    13141 tgcccggcac gcgagccgct ttgattgtac gggtcagaac agcttcagaa gcgttgtggg
    13201 gggccagacc ggttcaaaaa ccgtgacgcg caggtcgtcc acgaatgccg tgccgttggt
    13261 gatggatggc tgggcgacgg tgccggtgcg gaagatcacc atagcctgaa ctccgctgac
    13321 accggccgca catccccccg gaaaaccggt tcgtccccgg cggcggcgat ctccgccatg
    13381 cgggtctgca agacactcgt gccattgagg tggaagagat cgttgctgta attgatcttg
    13441 ctggtgtcgg taaaccagtt tgcgagaccc tccccggaat gattggtgtg cgccatcacc
    13501 aaagacgaac cgtacccggc tttcagtccg tcggcgattg cggtggccca gacggctttg
    13561 gcattcgact ggccgccggc gaaaaagaca ttcacggttt tcgattcggc ccggcctccg
    13621 ataagtataa gcgcgacagc gattccagcc cactggcatc ttggtctcat catctttcct
    13681 ttcgcctgca caaccctgtc catacgatac gggtggcggg gtaacgttcc cccgaacccg
    13741 atgtgttgca tatcttcgca gttcatctcc ggggacgtca atctggactt tgcgttcgac
    13801 acatcgctct ttttttcgca caatctcaaa ccgtaaagga ggtcgagtta tggaacggaa
    13861 cggaacgaga cggggatttc tccggatggg ggcgtgcggc gcttcggttt gggcgctcag
    13921 cgggggtgta cggcaggcgg agggggcgtc gacggacaaa tcgttcgggc acggatcgtt
    13981 cgcgttggac ggtaaccgga ttcgggtgtt ttccccggcg gtccgcgagc cggtcaaggt
    14041 gtgggttgcg ggtgacacgc atctgcaccg tgatgacgcg cgtggcgaac cgttccgcga
    14101 atacagcagc cgtatggcgg ggggctacaa ccgtgtgcgg catgtcgaga gcggcgagct
    14161 caccacgccg gaggccagtt tcgaggagac gctccggcgc gcgaaggagg cgcgcgtcga
    14221 tctgctcgcg ctggtgggcg atgtgttcag ctttccctcc gaggcggcga ttgaatgggc
    14281 acaggccaaa ctcgatgcgt caggtgtgcc ctacctctat gtcgcgggca accatgactg
    14341 gcactacgag gggttgcccg gatcgagcat ggccctgcgg agcgaatgga cggaaaagcg
    14401 gctcaagccc ctatatcagg gacagaaccc gttgatggcc gtccgcgaaa tccgggggat
    14461 ccggtttgtg gcgatcgacg attcgaccta cgagatcctg cctgaacagc gggcgttttt
    14521 ccgcgaacag gtggccgaca acacgccgct ggttctgctc atgcacattc cgctctacgc
    14581 gcccggccga ccggtcggat tcggttgcgg ccatcctgac tggggtgccg cgcaggaccg
    14641 gcactggaag atcgagcgcc gtcctcaatg gcctgccgca ggccacacgg cggtgacgcg
    14701 cgccttccgc aatgaggtgt tcagcgcgcc cggcattctg ggcgtgttcg cgggccatac
    14761 ccatgtgcaa tcgctggatg tggtcaatgg tgtgccgcag gtcgtcaccg ccgcgaatgc
    14821 cggctgcggc atggtgcgcg ccgaatttct gcctgcgcac aacggtcatt gacaagctgg
    14881 cgccgattta ggatgctata cgcacagtcg tcgtaaggat aaccgcaagg gaggagatat
    14941 ggctaaacgg aaaccggttc agaaacgcgt cgccatcgtg gggttcgggt ttatggggcg
    15001 catgcattac gggcagtgga aaaagatccg gaacgcgaaa atcgtggcgt tgtgcgatca
    15061 gaatcaggcg cagttcacgg ctccggttca gggcgggaac gtcgcgggtg cggacacctc
    15121 gatggattac ggggatgcgg tgatttatga cgacttcgac cggatgttga aggaggcccg
    15181 cccggatgtg atctcgctga ccctgccgac gccgttacac gttccgttca cggtcaaggc
    15241 gctgaaagcc ggggtgagcg tgctgtgcga aaagcccatg gcgttgaatg cggttgattg
    15301 cggcgtgatg ctgcgcgccg cgaaacaggc gccgaagggt acgcggctga tgattgccca
    15361 gtgcctgcgc ttctggccga gttacgtgta tctcaaggat ctgattgata gcgggaagta
    15421 cggcaaagtc atcgcggcct ccttctaccg tttcagcgcg cctccgggct ggcaaaaagg
    15481 ggtcaactgg ttcatggatg agtcgaaaag cggcggcgtg gcgctggatc tgcacatcca
    15541 tgacacggat atcgttcagt ttttgttcgg catgccgcgc gcggtgacga gccgggctaa
    15601 ctttgcgaaa gacggggcga tgcgctacat ctccactctg tacgatgtgg gcggtgcggc
    15661 ggtgacggcc gaggcaagtt gggccatggc gccgacgttg ggcttcgagg ccgggtatgt
    15721 ggtgacgttc gagaaggccg tagtcgtgct ggacgataaa cgcgggaaac cgctctgcgt
    15781 ctatcctgcc aaaggaaagg cgttcgtgcc gaaactgccg aaaggcgacg gctacgagta
    15841 cgagatccgc tggttcatgg atctgctgtg cggccgcaag gtcgcgtgtg tgaccacgcc
    15901 ggaacagtcg cgcgattccg tgcggatcgt ggatgctgaa aaacgttcgg cccggacggg
    15961 cagaaccgtg cgcctcgcaa agtaagcgaa aaaagagaag cgaaaacatt gggcccgttc
    16021 gccggacccg cggaggaaag atcatgttaa agtgcggaaa atggccagtg gccgtgtgca
    16081 gttggtcgtt cagaaaaagc gtggaggaga tcgggtctgt gatgaccgcg ctgggattgg
    16141 agcatatcaa cctggcgatc gccccggctc tggcgccgga cggcgaggcg tatcttgatg
    16201 ccgttcgaaa gcagggctgg actgtgacag ccggcatgat gaattacgac tatgaggatt
    16261 acagcacgtt ggacacgatc aaggtgaccg gcgggatcgc gccggatgcg tactggcccg
    16321 cgagcgggga ggcgttcgcc cgtgcggccc agatcacggc tgcgctcggc tcgcagtaca
    16381 tcatgatgca cgtggggttc ttggatcaca ctgacaacgc ctacgctaaa aaattctatg
    16441 accgtgtgcg cgcgctgggg gattcggcgg gtgaggcggg cgtgacgctg ctgatggaga
    16501 ccggacagga aacagccgag gagcttcagc ggtttgttga gaccctgaac catccgaatg
    16561 tgctcttgaa cttcgatccg gctaatctca ttctgtacaa caaggatgag ccgcttccgg
    16621 cgctgcgtcg cctcgcgcct tggatccgcc acattcacat caaggatgcg atccgaacca
    16681 ctgtgcccgg cacatggggg agcgaagtcg tgtggggcga agggcaggtc aatgctttcg
    16741 cgttcctgaa cgtgctggag gagatcgggt acgagggtgc cgtggccatc gagcgcgagg
    16801 cgggtgagca gcgcgtccgc gatatcgcca cagcggtgcg ccgattggtc gcctattgag
    16861 gtcactcctc ttcgatcagc gtcagggtca catcccgccg tttgccgtta cgggcaacgg
    16921 ttagccggac ttgttcgccc ggccgacggg ggtcgaggac ggcctgtagc tcttcgatcg
    16981 tgcccaccgg gattccgtcc acggcttgga tcacatcgcc gaaaatcaga tctccgagcc
    17041 gactgacgcc gatcccttca agacctgcgc gtgcggcggg tgttcgttcc gcaaccgcgt
    17101 acagggccac gcccgcgatg cccgcgcgtg cggtcacatg atcgggcaac agatacacgc
    17161 cgagtccggc acgcgtgacc cggcccttct caatgagttg cggcaccacg cgggacacgg
    17221 tgtccgaggg gaccgcgaag ccaatgccgg catacgcgcc actcgggcta aggatcgcgg
    17281 tgttgacccc cactagcctg ccggatgagg aaagcagggg accgccggag ttgccgggat
    17341 tgatcgcggt gtcggtctgg atgacatcgt aaatcttgcg gtcggtcatg gaggtgatgg
    17401 tgcggccgag tgcgctgacc agaccggccg atagggtggt gtcgagacca aacgggttgc
    17461 cgatcgcgta aacctgctgt ccgacctgca acgcgcgtga ggtggcgacc gtgaccggca
    17521 cgagtttctc ggggggtgcg ttgatcctca gcaccgccaa atcataatcc ggcgcgatac
    17581 cgaccagtac ggcgtcgtac cgtgatccat ccgggaaggt aacggtcagt gtgctggctt
    17641 gatgcaccac
//
LOCUS       DAOSLE010000053        17617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3428_length_17617_cov_6.809703, whole genome shotgun sequence.
ACCESSION   DAOSLE010000053 DAOSLE010000000
VERSION     DAOSLE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17617
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_17617_cov_6.809703"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PK576_05280"
     CDS             <1..463
                     /locus_tag="PK576_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61039.1"
                     /translation="RYRDRDRLFHHRTLSWVSWFQGFGVSGFQGFRVSSGSGSLSGSG
                     SIVSPQNTFFGFKVSGFKVSWFQGFWVSGFMVSGFMVSSGSGSLSLSGSTVSPQPHSL
                     TAFEVAGFARISIRQPCDAGADGPESGAARWRGGFPANRRRSILLLLGLIL"
     gene            complement(552..2177)
                     /locus_tag="PK576_05285"
     CDS             complement(552..2177)
                     /locus_tag="PK576_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOE61040.1"
                     /translation="MTLPVTFLPTGKTVRVPCGTSLLQAAVQAGLLIDTPCGGSGTCG
                     KCRMRIRAGEVSAGGSSQHLTAEQLEAGWRLACVTRVTGPLEVEVPGTSLLARLDTIL
                     VNGAPVSIRRDPHRLGDMGVAFDLGTTTVAGTLFDLHTGLERASHAVMNRQVRMGDDV
                     ISRIHAVRQDPSTLTQLQTAAIETLNEILDALCDACGETSGTVRRVTVAGNTAMQQLL
                     LGIDPSPLGEAPFTAAFTDAQTTGAARLGLKVAANAMLTVFPQIGGFVGGDTVAGLLA
                     SRFDTLTHPALLVDIGTNGEIALFTGNALLVASSAAGPAFEGARIRQGMRAIPGAIDQ
                     IWMQDGDLRCHVINDAAPCGLCGSALVDVVSVLLQAGLIDETGLLAPPAAAPQETCRA
                     LHDRLIPFEDAWACVLARDACGEPLVMLTQKDIRELQLAAGAIRTGIEVLLARANLTP
                     RDLDALLLAGGFGNYIRREHALRIGLLPPVHELAVRFVGNASLSGAKRALLAQAELDR
                     AQQLRNRATHVELAADPRFSELFMQHMSFPAGS"
     gene            complement(2189..3001)
                     /locus_tag="PK576_05290"
     CDS             complement(2189..3001)
                     /locus_tag="PK576_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE61041.1"
                     /translation="MSVKTMLFVDGSWLYHSRQVLFESLGEESGFEIDYKRIPDIIAH
                     EIADVLDAEVDVVRTNYFGTIPVNKQGYNPSKQKAFYDFLALQCAYDTEILEIDFRRE
                     PQARPDDKWVNVALASSMLYFAAVPGAYDVATLVGGDADYIPLLKRVRAMGKRVQVVG
                     MNNIDGKFLTSAMLLTTSGIQDLPPIFLDEHAQDIRLVREEQTRACKNCGCEERTTWA
                     GPDFFCSNCRSEHRKQIRVCDTCGCEEETTWDKPFFYCAACRSKYREGENNS"
     gene            complement(3105..3563)
                     /locus_tag="PK576_05295"
     CDS             complement(3105..3563)
                     /locus_tag="PK576_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOE61042.1"
                     /translation="MILDTLDSSFRYETLHPLFPSAFAFLRRPDLDALACGRIDLAGD
                     DALYALIQTYETQPPAEGKPEAHRRYLDIQYVVSGSECVAYAPLAGLTPCQPFDEAGD
                     YGLYTGSTSLVRLSQGMFAIFYPNDAHLPCRQDGHAAPVRKIVIKVKVSE"
     gene            complement(3560..4120)
                     /locus_tag="PK576_05300"
     CDS             complement(3560..4120)
                     /locus_tag="PK576_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOE61043.1"
                     /translation="MNTARSPSPRDPLRRGPHGGLVLFVCTGNTCRSPMAEALFRAAV
                     PPDSSWRAASAGLAAEEGKPMSAPARVVLAEMAGCVDFADHRSQPVTGKLVADARMIV
                     VMTGAHARQLTARFPEARDKLALLRAFDSAAPAYCDLDDPFLGSPDAYRACRDHIRAA
                     LPGLIRYLTEGGGSCLPSSAIHQETP"
     gene            complement(4175..4309)
                     /locus_tag="PK576_05305"
     CDS             complement(4175..4309)
                     /locus_tag="PK576_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61044.1"
                     /translation="MKAPPVTPAGVTAPDAPHGMVVGTLPIRTKNLGNLRWHVLLPAE
                     "
     assembly_gap    4576..4675
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4676..5424)
                     /locus_tag="PK576_05310"
     CDS             complement(<4676..5424)
                     /locus_tag="PK576_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61045.1"
                     /translation="MAYSTAMNPIPPKHTPGSTGEDTPETGGGLVAMYHAASQANAES
                     FPVLKAFQDYLEAERSRARRRVTQLSVFFAVLMGVVVSGFLTAGIFMWRNMAAVQNKL
                     LDVALATREPAPQPLPQPAQPPAVSPILEESVRQMSRATVALQDNLDRKLNGVSVLTE
                     KVHARVASQDSELEKLRGEMRSMLEQSAKLKDELVTLRNNVNKPAPPQVAAPAPAQPA
                     PPPPAQPPAPAVAAKPAASVSAPPVPAAPAPA"
     gene            5541..7235
                     /locus_tag="PK576_05315"
     CDS             5541..7235
                     /locus_tag="PK576_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE61046.1"
                     /translation="MPGQAEALAALMLKKTGLFTDTQISSVLAADHSDGTGVAGAVVR
                     LGLAREEDFLRKIGEMLGFGYTDLEVTRPTDEAITRLPARAVYQYNVLPLSVENGVLT
                     VVTCDPFNTGMTDGLRLAAGCPVRVTLSPREMIDKAIRKYYGVGADAIEKMIEDGRYE
                     VDADLASISKIDVNEMGAEASIVKFVNQIIAEADRQGATDIHVEPMEDELRIRYRIDG
                     MLHKVDVPPQLNRLKAAILSRIKVMANLDIAEKRLPMDGRIGVRLNNDDIDIRVSTMP
                     TAYGESISLRLLQKAGNFVKLSDLGFNDFDYQHVVKLINRPNGIFLVTGPTGSGKSTS
                     LYAFLHEINKIQVRILTAEDPIEYEMPGINQVLVRQDIGLTFARALRAFLRQDPDIIM
                     VGEIRDGETAEIAINASLTGHLVFSTLHTNDAAGAFARLVDMGAEPFLVASAVAGVMA
                     QRLVRRLCPVCKKEIPLDRKWYDLPYPPAKETVWVPEGCDACTRTGYRGRGGLFELLV
                     VTEDIESAIIGRRSSGEIRDIAIGHGMKTLREDGWLKVFKGMTSVEEVMRVTEDVT"
     gene            7379..8626
                     /locus_tag="PK576_05320"
     CDS             7379..8626
                     /locus_tag="PK576_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE61047.1"
                     /translation="MASFSYKALTKDGRKVEGVLEAGDRRAALVAVERLGYTPISVME
                     SKTRKAKAKGVPFWKLKLGSGEQRMKPVEVLLFTSELSDLLEAGMTLGAALNCLANQG
                     EEDSPQSRIASDLRDRILRGEPFSDAVTAHPATFPPLYGNMIRAGEASGAMIEVLRRL
                     VEHYERNENMRSKITSALTYPTVVLIFGVLTVIFAMVKVIPQFAKIFEGMGTALPTPT
                     LILVGMSDFVVKYGVFLVAAIGIGGVMFKRYLKTDTGRLWWDGLKLKAPLIRGIVACG
                     AYSSLAYTMKTLLANGVNVLQALRIAEETCGNALIAEALRNARQRVTDGTTISGPLAA
                     SKVFPRMMTDMLAVGEQSGDMPSSLGHIGRRYENEMDRNIKIFTNALEPILIVLIGGV
                     VGFVAVSILMAVFKVTSSLSPAG"
     gene            8676..9077
                     /locus_tag="PK576_05325"
     CDS             8676..9077
                     /locus_tag="PK576_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HOE61048.1"
                     /translation="MKKERDEKRRRSRRGGFTLVELLLVVCILGILAAVVIPNIMGHD
                     EEARRQATRTSITAIEQAVQIFAMRHNGKLPDSLDELTVGTDDAPGLLKEGALNDSWG
                     TPFTYTKTGKKFKIVSAGPDGEVGTDDDLTN"
     gene            9172..9786
                     /locus_tag="PK576_05330"
     CDS             9172..9786
                     /locus_tag="PK576_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61049.1"
                     /translation="MRRGFTLVELLVVIVIIGVMALMIGPTFTTGSDMTRMRTASRGV
                     MQMARYARTMAVLYQTPMVLVIASDGGLRVEQGQGGKGAPPVSVASDASGQSAESGGA
                     SYMMTDLDAEKVYERVRFRVALDEECLDGDELGMEKLEREESWAGEGDAEEAVVRVRI
                     PFESNGRCLPFTVRVFAAGDADEADVLTVRVDRFGVARIVGDEA"
     gene            9786..10238
                     /locus_tag="PK576_05335"
     CDS             9786..10238
                     /locus_tag="PK576_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61050.1"
                     /translation="MRRNGFTLIEILIATMILAFGLVSLMVGLGHCAQMMMLSKTYQD
                     AQFVFSLGERLYPVPPADEIQDAEDDEKLNIDPVTVEEMAAELDEDLPRERLKALEGF
                     TFERHVDEREEPETNVVDDKLFVLRTRVSWGSGKEGYYEELVRFIRPK"
     gene            10270..11154
                     /locus_tag="PK576_05340"
     CDS             10270..11154
                     /locus_tag="PK576_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61051.1"
                     /translation="MMRRDRNGFTLLELTLAIMILSIMMLLSFFCFDAVVQSWNAGLE
                     MSDSLAQADYVMEQIVSGLRSAYYPHAGIQTEQYGFQHTDGGEGEEATDTISWTKIGR
                     ALVGERTQLAEVPHRVSLSVEQASREHDGGLMAKVWRDALQLEDFDPEKDVKPFLLAP
                     RVIGLDCRILKEPPERNATELDWEDTWSESNTIPYKVSLTLYMRPAKEREAPLAVTRD
                     VEIPLWDISQNPVKQGRGAGTGSRDAPGGRSRGGQQPIPPARGGGTQSGARSATGGGR
                     PGGGGPPGGGGPPGGPMQ"
     gene            11151..12281
                     /locus_tag="PK576_05345"
     CDS             11151..12281
                     /locus_tag="PK576_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOE61052.1"
                     /translation="MNVQRSTLNAQRSSENRGSALIIALWTIALLSLLVMSFALDALL
                     EGRVNLYVRQRRQVDYLTQSGIVIAEMLLLEYRNATASTTTDEKEDRWLGAKLDLQKG
                     EASTGDVVVDAANPDGGVVRVKISAGESSRWPINLLFHTGAGGAQVDKIWENVLTVAN
                     VPQEYWEELIDCWTDWVDADNTVTGRNGAERDYYEGLEQPYTARNGPIDTVDELLKVK
                     GFLPAIVNGGVLNPEEKDPKKQIVIRSGGIKAFFDIYGELVKINVNSADKEVLLTVPG
                     IDGDELLAGAIVEERETGSRRVSSAATDLDRESKLFKDWNDLTARIEGIPLTASDFLS
                     YAPEKYFKIEVEGEAGGISHRIEAMAIVEGDKVRYVRWREDP"
     gene            12464..13864
                     /locus_tag="PK576_05350"
     CDS             12464..13864
                     /locus_tag="PK576_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61053.1"
                     /translation="MSRPSEIIALLAEGGVLRGVRFAPRGRDAWTRTGGGVWPLEDAE
                     VDVAEGAAETEQGTVVESDTPLARAFSAARNALGLRRVVIALPLSRLLVRVLSFPVEM
                     REDLAEAVTLQMDKLSPFPGEELSVGFEIVSETETSLRVFAAALPEAVFEDLGGALRA
                     SGLQVLRTDASILGWFRTVRGPCHLDRPGRRVVLADPDGGWDLLVLDQGVPVLVRGLG
                     ALPDDESLVREVMLSLLNAELEAGHGAVAELVILSHQPPTAALRERLEKLTGAPVRFE
                     TPPSEDGGVEGVALRTGEGATLDLTPQVWRDAMKEEAVRRRVLTGVVAALVVWVLGMG
                     VLFSGPVVYRQLTARQNQASRAHFKAHKAVADTRERVNLIQAYTDRTYSALEMLKLVS
                     ERLPPGITLAGFTYRRDDGVRISGDAVQPTLVYDFKNAVTESPLFETVGLVGPSASKG
                     LHKFDVDAKFKGVTQK"
     gene            13861..14541
                     /locus_tag="PK576_05355"
     CDS             13861..14541
                     /locus_tag="PK576_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61054.1"
                     /translation="MSAVSSREKNMLLITAVLVLYAVAALSYKKQAANWKAARRTFMA
                     AQRKVTEERELIAARDMWAERYEAMRELMPIFPYEKDVDTHWLNLMDSTATRNGFAIS
                     RRQANKEQEVGDVYELPIECKDWEAPLESLVRFLYDLHQQGAMLDVRQLFIRPSTKPG
                     YLKGSFTLYCAYMRGDAESLPPPSVSAGAETNGLPPTADGTVTNSAAIPPPGTGIAVT
                     NPVAGEGS"
     gene            14673..>17617
                     /locus_tag="PK576_05360"
     CDS             14673..>17617
                     /locus_tag="PK576_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOE61055.1"
                     /translation="MKNMRNDWVVFGAVCALALGVTTLAQDPAPDAAAAPAAGDANPA
                     PAPVVEVPAPQRALNSRSSRLSRPPLRSLRATAGAPASVAALSAGDATEPPGETGAEP
                     KPKTTTPSLKFDNAPTDIILQAYAQETGKTLLIAPDAPKANITLKSQPDVALSREEFI
                     EAIEVVLSMNGIVLEPFGEKFVKVLARKTVRTDGIKILMESPEKGHPEKNRVVSQMIQ
                     LKAITAEEAQKALEGFKKPDGLFQMFERTNSILVTDTQENINRMLEIIKFIDQPLVVL
                     EEVNVRAIRYAKAEEIKKRLEEIVAESQKQTQAKEEIKPNVSGAPGFSRTTSRPLPPG
                     LSRLRAQQPEPAAAAPNENLATLISDADRGMIRGKVQILADERSNQLIIVTRADNMTF
                     FDKIITVLDVETAPDVKVEVRRLEYADAEEVSTMLNDLIGNASSKKEDSAPAGAPKAP
                     AGDSGRSTSLAEAVAARAGRMASAGADVGEAGKSKLGQLSKDNIKILADKRTNAIVMM
                     GSPNDLAAINEVIKGMDIQLSQVLIETVVLQVDLGDDLQTGMDWVQRGKQWQYVDDTS
                     KPIYETHPDTGVKTRIGYEQMRVRTRGTIFNNGDQLGGGGGGDDGNTKGLFGSLLGLG
                     TAVLSNGVASGYLGAPNGISYFLKSDKLNIAALIEASKSDSRTKVLSSPVLMTVDNKE
                     ATLEATTMRYLYKGVRYSGGSIDNREVPDFEQRDIGLTVKITPRINPNGAVVLTIEET
                     FETVGADQNVGGAKYPTVNTRKLQADVAVENMQTVVLGGLVQNEIKTSDSGIPILKDI
                     PYIGKYLFGKTSFSDNRSELLVFLTPYVLDTPQDVEAEAARRKAALSDDQAWVDYGWS
                     KSKLADPMSSSEKVRRTKQRWATEDANHKAEKEYERLLDAREEQLRERARKDAEDVLK
                     RAKQDAEKTAEGSDDDDDTEAKLRDLLEKLKAKKARESTAAAPVPDATESVIRVTDSV
                     EILGKGAGSP"
BASE COUNT         3382 a         4784 c         6075 g         3276 t
ORIGIN      
        1 tcgctatcgg gatcgggatc gattgtttca ccacagaaca ctttcttggg tttcatggtt
       61 tcagggtttc ggggtttcag ggtttcaagg tttcagggtt tcatcggggt cgggatcgct
      121 atcgggatcg ggatcgattg tttcaccaca gaacactttc tttggtttca aggtttctgg
      181 tttcaaggtt tcatggtttc aaggtttctg ggtttctggt ttcatggttt ctggtttcat
      241 ggtttcatcg gggtcgggat cgctatcgct atcgggatcg actgtttcac cacagcctca
      301 cagtctcacc gcctttgagg ttgcgggctt cgcccgcatc tcaatacgtc agccctgcga
      361 tgctggcgcg gacggaccgg aaagcggtgc cgcccgttgg cggggcggtt tccccgcaaa
      421 ccgccgccgg tccatactgc tccttttagg gttaatcctg taatcctgtc aatttatagg
      481 caaggttatt cgaacctgcc ggatctgccg ttacgatcgg tggggtcgaa ctcatgcctc
      541 tgcgcgcatc atcaacttcc cgccgggaag ctcatgtgct gcatgaacag ttcggagaag
      601 cgcgggtcgg cggcgagttc cacatgggtg gcgcggttgc gtaactgttg cgcccggtcc
      661 agctcggctt gtgcgagcag tgcgcgtttc gcgccgctca gcgaagcgtt cccgacaaac
      721 cggacggcga gttcgtgcac tggcggcaac aggccgatgc gcagcgcatg ctcgcgccgg
      781 atgtaattgc cgaacccgcc agccagcagc agggcgtcga gatcgcgcgg cgtgaggttc
      841 gcgcgggcca gcagcacctc gatgccggtg cggatcgcgc ctgccgcgag ttgcagttca
      901 cggatatctt tctgtgtcag catcacaagc ggttcaccac aggcatcccg ggccagcaca
      961 caggcccagg cgtcttcgaa ggggatgagg cggtcgtgca gcgcgcggca ggtctcttgc
     1021 ggtgcggcgg caggaggggc cagcaggccc gtctcatcga tcaagccggc ttgcagcaag
     1081 accgacacca catccaccag cgccgagccg cagagcccgc acggggccgc gtcgttgatc
     1141 acgtgacaac gcaggtcacc gtcctgcatc cagatctggt cgatcgcgcc ggggatggcg
     1201 cgcatgccct gacggatccg cgcgccttcg aaagccggac cggctgcgga agaagccacc
     1261 agcaacgcgt ttcccgtgaa gagcgcgatc tccccgttgg ttccgatgtc caccagcagg
     1321 gccggatgcg tgagcgtgtc gaagcgggat gccaataggc cggccaccgt atcaccgccg
     1381 acgaacccgc cgatttgcgg aaagacggtg agcatggcat tggccgcgac cttgagtccg
     1441 agacgggccg cacccgtggt ctgcgcatcg gtgaaggccg ccgtaaacgg tgcctcgccg
     1501 agcggggagg gatcgattcc cagcagcaac tgctgcatgg cggtattgcc cgcgaccgtc
     1561 acccgccgga cggtcccaga cgtttcaccg caggcgtcgc acaacgcgtc gaggatttcg
     1621 ttcagggttt cgattgcggc ggtttggagt tgcgtcaggg tggagggatc ctggcggacc
     1681 gcgtggatcc ggctgatcac atcatcaccc atccggactt ggcggttcat caccgcatgc
     1741 gacgcgcgtt cgagaccggt gtgcagatcg aacagggtgc cggccacggt ggtggtgccg
     1801 agatcgaatg ccacgcccat gtcaccgagt cggtgcgggt ctcggcggat cgacaccggc
     1861 gcaccattca cgagaatggt gtccagccgg gcgagcagcg aggttccggg aacctccacc
     1921 tccagcgggc cggtcacgcg cgtcacgcag gcgagtcgcc acccggcctc aagttgttct
     1981 gcggtcaggt gttgcgacga gccgcccgcc gaaacctccc cggcgcggat ccgcatccgg
     2041 catttgccgc aggttccgct gccgccgcat ggggtgtcga tcagcagtcc cgcctgaacg
     2101 gctgcctgaa gcagggaggt gccgcatggg acacgcacgg ttttgccggt cggcagaaac
     2161 gtgacgggaa gagtcatcac gactggagtc aggagttgtt ttcgccttcg cggtacttgc
     2221 tgcggcaagc cgcgcaatag aagaacggct tgtcccaggt tgtctcttcc tcgcatccgc
     2281 aggtgtcgca cacgcgtatc tgtttgcggt gttcgctgcg gcaattggag cagaagaagt
     2341 ccggtcccgc ccatgtggtg cgttcctcgc atccgcagtt cttgcaggcg cgcgtctgct
     2401 cttcccgcac gagccggata tcctgcgcgt gctcatcgag gaaaatcgga ggcagatcct
     2461 gaataccgga agtggtcagc agcatggcac tggtgaggaa cttgccgtcg atgttgttca
     2521 tgccgacgac ctgaacccgc ttgcccatgg cgcgcacacg tttcagcaac ggaatgtagt
     2581 ccgcgtcgcc gccgaccagc gtggccacgt catatgcgcc gggcacggcc gcgaaataga
     2641 gcatcgagga ggccagcgcc acgttgaccc atttgtcatc cggccgggcc tgcggctcac
     2701 ggcggaaatc gatctcgagg atctccgtgt catacgcgca ctgtaaggcc agaaaatcgt
     2761 agaaggcctt ttgtttggag gggttgtaac cctgtttgtt caccgggatg gtgccgaaat
     2821 agttggtacg gacgacatcg acttcggcat ccagaacgtc ggcgatctca tgcgcaatga
     2881 tatccggaat ccgtttgtaa tcaatctcga atccgctttc ttcccccaat gactcgaaga
     2941 gcacctgccg actgtgatac agccaactgc catcgacaaa gagcattgtt ttcaccgaca
     3001 tgcgtaatta cctttcttga gttcgctatg ttccccgcac accttgcgtt ttcggcgtgc
     3061 gtcgatcagg acaggcacag aatgccaaaa cccggcgcgg gtgtctattc cgaaactttt
     3121 actttgatga caatttttcg taccggcgcg gcatgcccgt cctgcctgca gggcaggtgc
     3181 gcatcgttcg ggtagaagat ggcaaacata ccttgagaga gtcgcacgag agaggtggaa
     3241 ccggtataga ggccgtaatc cccggcttcg tcaaacggct ggcagggggt gagcccggcc
     3301 agcggggcat aggcgacgca ctcgctgccg gacacgacat actggatgtc gaggtagcgg
     3361 cggtgcgctt ccggtttgcc ctctgcgggc ggttgggttt catatgtctg tatcagggcg
     3421 taaagcgcgt cgtcgccagc caggtcgata cggccgcagg ccagcgcgtc gagatcggga
     3481 cgccgcagaa acgcgaaggc ggagggaaaa agcgggtgca aggtttcgta ccggaaactc
     3541 gaatccagcg tgtcaaggat catggtgtct cctgatgtat ggcagacgac ggcaggcagg
     3601 aaccgccccc ttccgtcagg taacggatga gtcccggcag ggccgcccga atgtggtcgc
     3661 ggcaggcgcg gtacgcgtcg ggggagccca gaaacggatc atccaaatca caatacgcgg
     3721 gggctgcgga atcaaacgcg cgcaacagcg caagtttgtc ccgggcttcg gggaagcggg
     3781 ctgtgagttg gcgcgcgtgc gcgccagtca tcaccacgat catcctcgcg tcggccacga
     3841 gttttccggt gaccggctgg ctccggtggt ctgcgaaatc gacgcagccc gccatttcgg
     3901 cgagcacgac acgcgccgga gccgacatcg gcttgccctc ttccgcggcc agtcctgcgg
     3961 aagccgcgcg ccaagacgaa tccggcggta cggcggcacg gaacaacgcc tctgccatgg
     4021 gactgcggca ggtgttgccg gtgcagacaa agaggacgag cccgccgtgc gggccgcgcc
     4081 gcaaaggatc gcgcggggaa ggggagcgcg ccgtattcaa cgcccaacgt tcccccttta
     4141 agttatggac gtgcggagac attacgaagt cgccctattc agcggggagc agcacatgcc
     4201 agcgcaggtt accgaggttt tttgtccgga tcggcaacgt gccgacaacc atgccgtgcg
     4261 gcgcgtccgg cgcggtcacg cctgcaggcg tgaccggcgg cgctttcacg gctggcggaa
     4321 accgcgcgtc acgcgcggaa tgcggcgcgg cagcgggttt ctctgcggcg ggttttgccg
     4381 cggtaaccgc cgaagctggt gcggatgcgg acggggctgc gggtacgggg ggcgcggaaa
     4441 cgctcgccgc tggtttcgcg gcaacggcgg gggctgcggg tgcaggagtt gcaggtgcag
     4501 gggccgcagg tgcaggggct gcaggtgcag gggctgcggg tacggggggc gcggaaacgc
     4561 tcgccgctgg tttcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcagg
     4681 tgcaggggct gcgggtacgg ggggcgcgga aacgctcgcc gctggtttcg cggcaacggc
     4741 aggggctggc ggctgggccg gcggaggggg tgcaggctgg gccggtgccg gtgcggcaac
     4801 ctgaggcggc gcgggcttgt tcacgttgtt acggagggtg acgagttcgt ctttgagttt
     4861 cgcgctctgt tcgagcatgg agcgcatctc tcctcgcagt ttctcgagtt cgctgtcttg
     4921 cgaggcgacc cgggcatgaa ccttttcggt gaggacgctc acgccgttca gtttccggtc
     4981 cagattgtcc tgtaacgcca cggtggcgcg cgacatctgg cgcactgatt cctcgaggat
     5041 cggcgaaacg gctggcggct gggctggctg cggtaacggt tggggcgcgg gttcccgcgt
     5101 ggccagcgcc acatcgagaa gcttgttctg cactgcggcc atgttacgcc acataaagat
     5161 cccggcggtg aggaacccgg agaccaccac ccccatcaag accgcgaaaa agacggagag
     5221 ttgtgtgacg cggcggcgcg cccgcgagcg ctcggcctcc agatagtctt gaaacgcctt
     5281 caatacgggg aacgattcgg cgttggcctg agaggccgcg tgatacatgg cgaccagacc
     5341 gcctccggtt tcaggcgtgt cctcgcccgt ggaaccgggc gtatgttttg ggggaatcgg
     5401 gttcatggca gtagagtacg ccatttgggg ttttccggaa aggggaaaaa tcagattagg
     5461 catggattgc gcgtcggctt tggtgtacac ttatccttta cgtttctgaa tcggaccagt
     5521 tcacagaaag ggatgtgctc atgcccggac aggcagaggc tttggctgcc ctcatgttga
     5581 aaaaaacggg gctcttcact gacacgcaga tcagcagcgt gttggcggcc gatcactcgg
     5641 acggaacggg tgtggcgggt gcggtcgtgc gcctcggatt ggcgcgtgaa gaggacttcc
     5701 tccgtaagat tggcgagatg ctcgggtttg gctatacgga tctggaggtc acccggccga
     5761 cggatgaggc catcacccgt ctgcccgcgc gcgcggtata ccagtacaac gtgctgcccc
     5821 tgtcggtaga gaacggggtg cttaccgtgg tcacctgcga cccgttcaac accgggatga
     5881 cggacgggtt acgactggcg gccggttgtc cggtgcgggt caccctcagc ccgcgcgaaa
     5941 tgatcgacaa agcgatccgc aaatactacg gcgtgggggc ggacgcgatc gagaagatga
     6001 tcgaggatgg gcgctacgag gtggatgccg acctcgcctc gatctccaaa atcgacgtga
     6061 acgagatggg cgccgaggcg tcgatcgtca agttcgtgaa ccagatcatt gcggaggcgg
     6121 accgtcaggg tgccacggac attcatgtgg agccgatgga ggacgagttg cgcatccggt
     6181 accggattga cggtatgctg cataaggtgg acgtgccgcc gcagttgaac cggctcaagg
     6241 ccgcgatcct ctcgcgcatc aaggtgatgg cgaatctgga catcgccgag aagcggctgc
     6301 cgatggacgg gcgcatcggg gtgcggctca acaacgacga tatcgatatc cgcgtctcca
     6361 ccatgccgac ggcgtacggg gagtcgatct ccctgcgtct gctgcagaag gccgggaact
     6421 tcgtcaaact gagcgacctc gggttcaacg atttcgatta ccagcatgtg gtgaaactca
     6481 tcaaccggcc caacggcatt tttctggtca ccgggccgac tggttcaggg aagtccacct
     6541 cgctctacgc ctttttgcac gagatcaaca agatccaggt gcggattctg acggccgagg
     6601 atccgatcga atacgagatg ccggggatca atcaggtgct ggtgcggcag gatatcggcc
     6661 tgaccttcgc gcgggcgttg cgcgcgtttc tgcgtcagga cccggacatc atcatggtgg
     6721 gtgaaatccg cgacggcgaa accgcggaga tcgcgatcaa tgcgtcgttg acgggacacc
     6781 tcgtgttcag cacgctgcac accaacgatg cggcgggcgc gtttgcgcgt ctggtggata
     6841 tgggggccga gccgtttctg gtggcgtccg ccgtggccgg cgtgatggcg cagcgcttgg
     6901 tccgccgcct ttgccccgtg tgcaaaaaag agattccgct ggaccggaag tggtatgatc
     6961 tgccgtatcc gcccgcaaaa gagacggtct gggtaccgga agggtgtgat gcctgcacgc
     7021 gtaccgggta ccgcggacgc ggcgggttat ttgagctgct ggtggtgacg gaggacatcg
     7081 agtcggccat cattggtcgc cgctcctccg gcgaaatccg cgacatcgcg atcgggcacg
     7141 gaatgaagac cctgcgtgag gacggatggc tcaaggtgtt caaaggcatg acctcggttg
     7201 aggaggtcat gcgggtgacg gaagacgtga cgtgaggatt taggatttag gatttaggaa
     7261 ttaggattta ggagtgagga gtgaggattt aggagtgagg atttaggagt tgagaaggct
     7321 cggttccccc aattcctaac tcctaaatcc taactcctaa ctgacagaga ctgggtgcat
     7381 ggcgagtttt tcctacaaag cgctgacgaa agacggccgc aaggttgagg gggtgttgga
     7441 ggcgggcgac cgccgtgccg cgctcgtggc ggtcgaacgg cttgggtata ctccgatctc
     7501 cgtgatggaa tccaagaccc ggaaggccaa ggccaagggc gtgcctttct ggaaactcaa
     7561 gctgggcagc ggcgagcagc ggatgaagcc ggtcgaggtg ttgctgttca cctccgagtt
     7621 gagtgatctg ctggaggcgg gcatgacgct cggtgcggcc ctgaactgcc ttgcgaacca
     7681 gggcgaggag gattctcccc agagcaggat cgcttcggac ctccgcgacc gcattctgcg
     7741 cggcgagccg ttttcggatg ccgtcacggc gcatccggcc accttcccgc cgctgtacgg
     7801 caacatgatc cgcgcggggg aggcctccgg cgcgatgatc gaagtgctgc gccgtctggt
     7861 cgagcattat gagcgtaacg agaacatgcg ctcgaagatc acttccgcgc tcacctatcc
     7921 gacggtggtg ctgatcttcg gcgtgctcac ggtgattttc gccatggtca aggtgatccc
     7981 gcagtttgcc aagatcttcg agggtatggg cacggcgttg ccgacaccca cgctgatact
     8041 ggtcggcatg agcgatttcg tcgttaagta tggcgtgttt ctggtggcgg cgatcggtat
     8101 cgggggggtg atgttcaagc gctatctgaa gaccgatacg gggcgcctct ggtgggacgg
     8161 ccttaaattg aaggcgccgc ttatccgggg catcgtggct tgtggagctt actccagcct
     8221 ggcatatacg atgaagacgc tgctggcgaa tggcgtcaat gttctgcaag ccctcaggat
     8281 cgcggaagag acctgcggca acgccctgat cgccgaagcg ctgcgtaatg cgcggcagcg
     8341 ggtgacggac ggcaccacca tttccgggcc gctggccgcc agtaaggttt tccccaggat
     8401 gatgacggac atgctggcgg tcggcgagca gtcgggggat atgcccagtt cattggggca
     8461 tatcggtcgg cggtatgaga acgagatgga ccggaacatc aagattttca cgaatgcgtt
     8521 ggagccgatt ctgatcgtgc tgatcggtgg ggtggtcggg tttgtggcgg tcagcatttt
     8581 gatggcagtg ttcaaggtca cctcgtcgct cagcccggca gggtaggagg cggggatttt
     8641 tgatgatagt cagacatcac aaaggaggga gcgcaatgaa aaaagagaga gatgaaaaac
     8701 gcaggcggag ccgccggggc gggttcacgt tggttgagct gctgctggtg gtctgtattc
     8761 tcggcattct ggccgcggtg gtgatcccga acatcatggg acatgatgag gaggcgcggc
     8821 gtcaggccac gcgcacgagc atcacggcga tcgagcaggc ggtacagatt ttcgccatgc
     8881 gccacaacgg caagctgccg gactcgctgg acgaactgac tgtcgggacc gacgacgcgc
     8941 cgggactgct caaggaaggc gcgctcaacg actcgtgggg gacgccgttc acctatacca
     9001 agaccggcaa gaagttcaag atcgtgtcgg ccggaccgga cggtgaggtc ggtacggatg
     9061 acgacctcac gaactagtgc gggaaaggcg actcgaatcg acccgcttcg gcgtgattcg
     9121 acccggatcg attcagatcg attcaaatcg attcgggtct tgaggaggcg catgcggcgc
     9181 ggattcacac tggtcgaact gctggtggtg atcgtgatca tcggagtgat ggctctgatg
     9241 atcgggccga cgttcacgac gggttcggac atgacccgca tgcgaacggc gtcccggggc
     9301 gtcatgcaga tggcgcggta cgcccgcacg atggctgtgc tgtatcagac gccgatggtg
     9361 ctggtgatcg catcggacgg cgggctgcgc gtggagcagg ggcagggcgg gaagggagcg
     9421 ccgcctgttt ccgttgcgtc tgacgcgtct gggcagtcgg ccgagagcgg cggagcgtcg
     9481 tatatgatga cggatctgga cgccgaaaag gtttatgagc gggtacggtt ccgtgtggcg
     9541 ctggatgagg agtgtctcga cggggatgag ttggggatgg agaaattgga acgggaggag
     9601 tcgtgggctg gggaagggga tgcggaggag gctgtcgtgc gggtgcgtat cccctttgaa
     9661 agcaacgggc gttgtctgcc gttcaccgtg cgcgtgtttg ctgcggggga tgcggacgag
     9721 gcagatgtgc tgaccgtccg ggtggaccgg ttcggggtcg cgcggatcgt gggggatgaa
     9781 gcgtgatgcg ccgcaacgga ttcaccctga tcgagattct gatcgccacg atgatcctcg
     9841 cgtttgggtt ggtgagcctg atggtgggct tgggccattg tgcgcagatg atgatgttgt
     9901 ccaaaacgta tcaggatgcg cagtttgtgt tcagtttggg tgagcgcctg tatccggtcc
     9961 cgccggccga cgagattcag gatgcggagg atgacgagaa actcaacatc gaccccgtca
    10021 ccgtggagga gatggcggcc gaactagatg aggatctgcc gcgtgagcgc ctcaaagcgc
    10081 tggaagggtt cacctttgag cggcatgtgg acgagcgcga ggagccggaa accaatgtgg
    10141 tggacgacaa gctgttcgtg ttgcggacgc gcgtctcctg gggtagcggt aaagaggggt
    10201 attacgagga gctggtgcgg ttcatccggc cgaaatgagg gaggaggggc cggagagggt
    10261 gtccggcaca tgatgcgcag agaccgaaac ggattcacac tgctggagct gactctggcc
    10321 atcatgatcc tgtcgatcat gatgctcctg tcgtttttct gtttcgatgc cgtggtgcag
    10381 agttggaatg cgggattgga gatgtccgat tccctggcgc aggctgatta tgtcatggaa
    10441 cagatcgttt ccggattgcg ttcagcctat tacccgcatg cgggcatcca gacggagcag
    10501 tacggattcc agcatacgga cggcggcgag ggggaggagg cgaccgatac gatcagttgg
    10561 accaagatcg gccgggcact tgtgggcgag cggacgcaac tggccgaggt gccgcatcgt
    10621 gtgagccttt ccgtcgagca ggccagccgg gagcatgacg gcggcctgat ggcgaaagtg
    10681 tggcgcgatg ccctgcaatt ggaggatttc gacccggaga aggatgtgaa accgttcttg
    10741 ttggcgccgc gggtgatcgg tctggactgc cggatcctga aagagccccc cgagcggaac
    10801 gccacggaac tcgattggga ggacacgtgg tccgaatcga acaccattcc ctacaaggtt
    10861 tcgctcaccc tctatatgcg gcccgcaaaa gagcgtgagg ctccgttggc ggttacgcgc
    10921 gatgtggaga tcccgctgtg ggatatctcg cagaatcccg tcaaacaggg gaggggggcg
    10981 ggcacaggca gccgcgacgc gccgggaggg cggtcgcgtg gcggacagca gccgattccg
    11041 cctgcgcggg gtggaggaac acaatcgggt gcgcgtagcg cgacaggtgg aggcaggcct
    11101 ggcgggggtg ggccgcctgg cggaggcggg ccgccgggag ggccgatgca gtgaacgttc
    11161 aacgctcaac gctcaacgct caacgctcca gtgaaaacag aggctcggct ctgattatcg
    11221 cgctttggac gatcgccctg ctgagtctgc tggtgatgtc ctttgcgctg gatgccctgt
    11281 tggagggccg cgtgaacctg tatgtgcggc agcgtcgtca ggtggattat ttgacccagt
    11341 cgggcatcgt gattgcggag atgttgctgc tcgagtaccg gaacgctacc gcttcgacca
    11401 ctacggatga gaaagaagac cgttggctgg gtgccaaact ggatctccag aaaggcgaag
    11461 cctccaccgg cgatgtggtg gtcgacgcgg cgaacccgga cggtggcgtg gtgcgggtca
    11521 agatctcggc cggggagtcc agccgctggc ccatcaacct gctcttccat accggggcgg
    11581 gcggcgcaca ggtggacaag atatgggaaa acgtgctgac tgtggcgaac gtgccgcagg
    11641 agtattggga ggagctgatc gactgctgga ccgactgggt cgatgcggat aacaccgtca
    11701 ccggccgcaa cggtgcggaa cgcgattact atgaggggtt ggagcagccc tacaccgcgc
    11761 gtaacggtcc gatcgacacg gttgacgagt tgctgaaggt caaggggttt ctgccggcca
    11821 tcgtcaacgg gggtgtgctc aacccggagg agaaagaccc caaaaaacag atcgttatcc
    11881 ggtccggcgg catcaaggct ttttttgaca tctatgggga acttgttaaa ataaacgtca
    11941 attccgctga caaagaggtg ttgttgaccg ttccgggcat cgatggggat gaattgctgg
    12001 cgggagcgat tgtggaggag cgcgagacgg gtagtcggcg tgtttcctcc gcggccaccg
    12061 atctcgaccg ggagagcaaa ctgttcaagg attggaacga tttgaccgcc cgtatcgaag
    12121 ggatacccct gacggcgagc gatttcctga gttacgctcc tgaaaagtac ttcaaaatcg
    12181 aagtggaggg ggaggcgggt gggatctccc accggattga ggcgatggcg attgtggagg
    12241 gcgacaaggt ccgctacgtg agatggcggg aggacccatg aggaagtgca ttcgtgcgtg
    12301 cttgagggta gggacgcctc cccgaggcgt ccgcggacgg ttcggggaac cgtccctgcc
    12361 gggaagcggg ccgctctagt gtgaagggcg ggcagcagga aatgaaagac gagaaacccg
    12421 tgatctgagt gtcgtgagtc gggtgccgga aaaggttgtt tgagtgagca gaccgagtga
    12481 gatcatagcg ttgttggcgg aaggtggcgt gttgcgcggg gtgcggttcg ccccgcgcgg
    12541 gcgcgatgcc tggacgcgca cgggcggtgg ggtgtggccg cttgaggacg ccgaggttga
    12601 tgttgcggag ggggcggcag agacggaaca gggtacggtg gtcgaaagcg acacgccgct
    12661 cgcccgggcg ttttctgcgg cgcgcaacgc tttggggctg cgccgggtgg tgatcgcgct
    12721 gccgctttcg cgtctgctgg tccgcgtgct gagttttccg gtcgagatgc gcgaggacct
    12781 ggcggaagcg gtgacgctgc agatggataa gctctccccg ttcccgggag aggaactttc
    12841 ggtcgggttc gaaatcgtca gtgagacgga gacgagtctc cgtgtttttg ccgcggctct
    12901 gcccgaggcg gtgttcgagg atctgggcgg tgcgttgcgg gcgtcgggat tgcaggtgct
    12961 gcgtaccgac gcttcgatct tgggctggtt ccgcacggtg cgcgggccct gccacctcga
    13021 ccggcccgga cgccgggtag tgctcgccga tccggacggc ggttgggatc ttctggtttt
    13081 ggatcagggt gtgccggtgc tcgtgcgggg gttgggcgcg ttgccggatg acgaaagtct
    13141 ggtgcgtgag gtcatgctct ccctgctgaa cgcggaactg gaggccgggc acggagcggt
    13201 cgccgaactg gtcatccttt cgcatcagcc gcctacggcg gctttgcggg aacggttgga
    13261 gaaactgacc ggtgcgccgg tgcggttcga gacgccgccc tcggaggacg gcggggtcga
    13321 gggggtcgcg ttgcgaacgg gcgaaggggc aacgttggat ctgacgccgc aagtctggcg
    13381 cgatgcaatg aaagaggagg ccgtgcggcg ccgggtgctg accggcgtgg ttgcggcact
    13441 ggtggtgtgg gtgctcggga tgggcgtgtt gttttcgggc cccgtggtct accggcagct
    13501 tacggcgcgg cagaatcaag cctcccgcgc gcatttcaag gcccacaagg cggtggcgga
    13561 cacccgcgag cgggtcaacc tcatccaggc ctatacggac cgtacctact cggcacttga
    13621 gatgctgaaa ctggtttcgg agcgtctgcc gcccgggatc acgctggcgg gctttaccta
    13681 tcgccgtgac gacggggtgc ggatttcggg tgacgccgtg caacccacgc tggtgtacga
    13741 tttcaaaaac gcggtgacgg aaagtccgtt gttcgagacg gtcggcctcg tggggccgtc
    13801 ggcgagcaag ggtttgcaca aatttgatgt ggacgcgaag ttcaaggggg tgacgcagaa
    13861 atgagtgcgg tctcctcccg agagaagaac atgctgctga tcacggccgt gctcgtgctc
    13921 tatgccgtgg cggcgctgag ttacaaaaag caggccgcga actggaaggc cgcgcgccgg
    13981 acctttatgg cggcccaacg taaggtcacg gaagagcgtg agctgatcgc ggcccgcgac
    14041 atgtgggcgg agcggtatga agcgatgcgc gaactgatgc cgatttttcc gtacgagaag
    14101 gatgtggata cccattggct gaacttgatg gattccacgg ccacgcgcaa cgggttcgcc
    14161 atttcgcggc gtcaggctaa caaagaacag gaggtcgggg atgtgtacga gttgccgatc
    14221 gagtgcaagg attgggaggc tccgcttgaa tcgctggtcc gttttctgta tgacttgcac
    14281 cagcagggcg cgatgttgga tgtgcggcag ctcttcattc ggccgtccac caagcccggt
    14341 tatctgaagg gctcgttcac cctgtattgc gcttatatgc ggggggacgc ggagtcgctg
    14401 cctccgcctt ccgtgtcggc gggggcggag acgaatgggt tgccgccgac ggcggatggg
    14461 acagtgacga acagtgcggc gatccctccg cccgggacag ggatcgcggt gacgaatccg
    14521 gtggcgggag aaggaagttg agagttgaac gttccaatgt ctggaggacc gtacacggtc
    14581 acagcgaagc ggtacatgtt cacgtacgcg aggatgagga tggaagagga agggcgtaaa
    14641 ggtaaggacc ttttggtttt gagggaacag gtatgaagaa catgagaaac gattgggtcg
    14701 tattcggggc tgtgtgtgcg ctggctctcg gcgtaacgac gttggctcag gacccggctc
    14761 cggacgcggc ggccgcgcca gcggcgggcg acgcgaatcc cgcacccgcg ccagttgttg
    14821 aagtcccggc cccgcagcgg gccttgaatt cacgctcgtc gcgcctgtcg cgtccgccgc
    14881 tccgttcgct gcgtgcaacg gcaggggctc ccgcttccgt tgcggcgctg tctgcgggcg
    14941 atgcgacgga acccccaggc gaaaccgggg cggagcccaa acccaaaacc acgacgccgt
    15001 ccctcaagtt cgacaacgcg cccacggaca tcatcctgca ggcctacgcc caggagaccg
    15061 gcaagaccct gctgatcgcg ccggacgcgc ccaaagccaa catcacgctg aaaagccagc
    15121 cggatgtggc gctctcgcgc gaggagttca tcgaggccat cgaagtggtg ctgagcatga
    15181 acggcatcgt gctggagccg ttcggcgaga agttcgtgaa ggtgctggcg cgcaagaccg
    15241 tgcgcacgga cggcatcaaa atcctgatgg agagcccgga aaagggccac cccgagaaga
    15301 accgggtggt cagccagatg atccagctca aggccatcac ggccgaggag gcgcagaaag
    15361 cgctggaggg gttcaaaaaa ccggacggcc tgttccagat gttcgagcgc accaacagca
    15421 tcctggtcac cgacacccag gagaatatca accgcatgct ggagatcatc aagtttatcg
    15481 accagcccct ggtggtgctt gaggaggtca atgtccgcgc gatccgctac gccaaggccg
    15541 aggagatcaa gaagcggctg gaggagatcg tggccgagtc gcagaagcag acccaggcca
    15601 aagaggagat caagcccaac gtttccggcg cgcccggctt ctcccgcacg acctcccggc
    15661 ccctgccgcc cggcctgtcc cggctccgcg cgcagcagcc cgagcccgct gccgccgcgc
    15721 cgaacgagaa cctcgccacg ctcatcagcg acgccgaccg cggcatgatc cgcggcaagg
    15781 tccagattct ggcggatgag cgctccaacc agctcatcat cgtcacacgg gccgataaca
    15841 tgaccttctt cgacaagatc atcacggtgc tggacgtaga gactgcgccg gacgtgaagg
    15901 tcgaggtgcg gcggctggaa tacgctgatg cggaagaggt ttccacgatg ctgaacgacc
    15961 tgatcggcaa cgccagcagt aaaaaggaag acagtgcgcc agccggggct cccaaagcgc
    16021 ctgccggtga ttccgggcgc agcacctccc tagccgaggc cgtggccgcg cgcgccgggc
    16081 gcatggcgtc tgcgggcgcg gacgtaggcg aggccggcaa gagcaagctc ggccagctca
    16141 gcaaggacaa catcaagatt ctggccgaca agcgcaccaa cgcgatcgtg atgatgggca
    16201 gccccaacga cctcgcggct atcaacgagg tgatcaaagg catggacatc cagctctcgc
    16261 aggtgctgat cgagaccgtg gtgctgcagg tcgatctggg cgatgacctg caaaccggca
    16321 tggactgggt gcagcgcggc aagcaatggc aatatgtgga tgatacatcc aagccaattt
    16381 acgaaacgca tcccgatacc ggagttaaaa cacgaatagg ttatgagcaa atgcgcgtta
    16441 gaactcgagg tactatcttc aacaacggcg accagttggg cggcggcggc ggcggtgacg
    16501 acggaaacac aaaaggcctg tttgggagtt tattaggtct cggcacggcc gtgctaagca
    16561 acggtgttgc ttccggctat ttgggtgctc caaatggaat cagttacttt ctcaaatccg
    16621 acaagctgaa catcgcggcc ttgattgagg catcgaagtc ggactcgcgg accaaggtgc
    16681 tctcctcgcc ggtgctgatg acggtggaca acaaggaagc gacgctggag gcgaccacca
    16741 tgcgttatct ctacaaaggg gtaagatact ctggaggatc aatcgataac agagaggtgc
    16801 cggacttcga acagcgggac attggtctga cagtcaaaat cacgccgcgc atcaacccga
    16861 acggggcggt ggtgctgacg attgaggaga cctttgaaac ggtgggtgcg gatcagaatg
    16921 ttgggggagc aaagtatcct accgtcaata cgcgcaaact tcaggcggat gtcgcggtgg
    16981 agaacatgca gaccgtggtg ctgggcggac tggttcagaa tgaaatcaag acatcggatt
    17041 ccgggatacc catactcaag gacatcccct acatcggcaa gtatctgttc ggcaagacct
    17101 cgttttcaga caaccgttcc gaactcctgg tctttctgac cccctacgtg ttggacacgc
    17161 cgcaagatgt cgaggccgag gccgcacgta gaaaggcggc tctgtccgat gatcaggcct
    17221 gggtggatta cggctggtcg aagagcaagt tggctgatcc catgtcatcg tcggagaagg
    17281 tgaggcgcac caagcagcgc tgggccacgg aagacgccaa tcataaggct gagaaagaat
    17341 acgaaaggct gctggatgcc cgcgaggagc agttgcggga gcgcgcgagg aaagatgcgg
    17401 aggatgtgct gaagcgcgcg aagcaggacg cggaaaagac ggcggaaggt tcggatgacg
    17461 acgacgatac ggaagcgaaa cttcgcgatc tgctggaaaa gctgaaggca aaaaaagcgc
    17521 gcgaaagtac ggccgccgca ccggttccgg atgcgaccga gtcggtgatc cgcgtcacgg
    17581 acagcgtgga gatcctcgga aagggggcgg gcagccc
//
LOCUS       DAOSLE010000054        17554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3445_length_17554_cov_8.114178, whole genome shotgun sequence.
ACCESSION   DAOSLE010000054 DAOSLE010000000
VERSION     DAOSLE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17554
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3445_length_17554_cov_8.114178"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /gene="lpxD"
                     /locus_tag="PK576_05365"
     CDS             complement(<1..960)
                     /gene="lpxD"
                     /locus_tag="PK576_05365"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOE61056.1"
                     /translation="MILTAIELAERLGGTLTGDGSVEVRALESLFAARPGDAAFFKDA
                     KYAAQLAATKASVVLVSETWQGTCPAPAVIRVPDPDGAFARAAVWFAPSPPERAPGIH
                     PTALIGRDVHLGASVHVGPYTVIEDGCVIGDGCVIEAQVFLGRQVSMGEGCHIYPQVT
                     VREGCILGCRVVLHSGVRIGGDGYGYTPKVAADGTVTIDKIPQLGIVELGDDVEVGCN
                     ATIDRARFGRTRIGNSTKIDNLVQIGHNVQVGSCSGIIAQAGVAGSTRIGSGCLIWAQ
                     AGISGHLEIGDRGQVGPKSGLAKDVPAGEYVLGLPAIPRREFAA"
     gene            complement(957..1553)
                     /locus_tag="PK576_05370"
     CDS             complement(957..1553)
                     /locus_tag="PK576_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE61057.1"
                     /translation="MKQMSGILAAVFAVTMAMAQGGTAVVNMVDLVRFHPSRERDRKL
                     MQDTEKEFQSKLDKQRDRFEQLRDDYEKAVKEARNPALNDKARAEAEDKAMKHRDVLA
                     EADRDLRQEMQKLQRDLGDLDTRLLRQVTSDIREVLSKFAQEKKYLVVLDGTTIAYFD
                     PKLDVTDEVLKRMGVDPKVRQEAKEKAEKEKAAAEKTK"
     gene            complement(1563..3926)
                     /gene="bamA"
                     /locus_tag="PK576_05375"
     CDS             complement(1563..3926)
                     /gene="bamA"
                     /locus_tag="PK576_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE61058.1"
                     /translation="MGKVMRAWLAVAVLAAGWVLAADNTVKEVQVRVLDQLGTDASDV
                     LSVCGVAAGSKLSQEALSKDVRALLDTGRYGYAGVELEKIEGGIRVIYVVRRRYRFQE
                     PLAIQGANYLSESKIRKLTELKSGDYIDEPVLSTQVAKVRDEYVKRYFPDVKIGMAIT
                     PIQGSAGFANVTLTIEEGERAKIRAYRFTGNTSIPEADLRSSFGQRPWYDPRGWFTDT
                     PVSRQDLEDARQQAEEVYQNHGFLDAKVASPVMEKVGKEKVDVVFAVTEGDVYHVEST
                     AIRGVKLFPEREVLASSRLKAEDLASRKAIDDAAKDIRDYYGSRGYVDTIVRPMKETV
                     PGKPGRMSIAFDVREGELVYIRNIVIRGNNKTRDKVIRREVLVNPGEIMNEPRIERSE
                     NRLKNLGYFANVRHYTEQTGQPGTRDLVYEVEEQRTGNFMIGAGFSSVDNLVGFMEIT
                     QSNFDILNWPNFTGGGQKARLGIEVGSRRQTAEVSWTEPWFLDRPLALTVEAYRRMRW
                     FDEYDEIRTGGSVGLSYPVKVGRVGLRYTMELVEMDDVESGEWYLDPKGDTASTPENW
                     NGDENDYFNWQEDEYGDALNSVIRLYWAHDTRDQPFVPTKGYQTTLFGELSEGAIGDN
                     EIYKLGVNYRHWFRMPWYKHVLSLRGRFETVDAYSGDVPIYEKLFLGGPRTVRGVEYR
                     DIGPKVYRGEGSKKHAPIGGQTLAMATVEYTVPVFKAVRVATFMDVGSIGEDVFDPEL
                     SDVCVSVGVGLRIDIPGFPIRFDFAKPVVEDDSYTDDEFFSFAIGFE"
     gene            4427..5770
                     /locus_tag="PK576_05380"
     CDS             4427..5770
                     /locus_tag="PK576_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61059.1"
                     /translation="MKKKTLAVVVCLFCSILSPVYARLVDTVPMIVRADSEATVTLTF
                     ADEPLLAKPDDVQVEYICADGLDSAGVPLGYGCFQPVASVVRDNTLTVSVRFRGETAH
                     TLRVIQKSSDPKKPNVLGIARLYSLCPDFFALRPYRGNMHMHSKFSDGNKNESPALMV
                     ATCRKLGHDFAIETDHRSYAGSLDAIEAFSKLPTDMKTFPGEEVHSPGNNVHILSLGA
                     SESITDWFTKSKTEYDQAVAAEQAKLPDTIPDRFKYPIAASFAVWDKIRACGGIAVFC
                     HPYWRPAYRQYIPAVVSDYLLQTAKFDAVEVLNTDSSELGILHYHELRAQGLKIAGIG
                     VTDAHSSKHLEPAYTLILAETLDFPSLAKNIRLRNCAAVDIDPVSKRQTVIGEFRFSR
                     YALFLLHHFYPLQNEVCKREGEWLLKALEGDEQAIASLKASQGTTPALRTKYWQK"
     gene            5797..6843
                     /locus_tag="PK576_05385"
     CDS             5797..6843
                     /locus_tag="PK576_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE61060.1"
                     /translation="MLTLILPALRGLMARGDTLPRLARVQPEGGEPLLTVPPGCDDAF
                     AVLQSHVPPGTSRVTLDTGGTYEAVFTDLPGRAAGRIALVTGAAQGFGFEIACDLAAQ
                     GAWVALADINEAGVLAAAEQVNEACGRSAAVGVAVNVADPDSARAAVAAAVTAFGGID
                     LLVSNAGVLRAGSVKTLPVKDLRFVTDVNYHGYFLMVQAVAPILALQHRAVPSRWFDI
                     IQINSKSGLTGSNKNGAYAGSKFGGIGLTQSFALELVEDGIKVNSICPGNFYDGPLWS
                     DPEKGLFKQYLDSGKVPGAKTFEDVKRFYESKVPMRRGCTTPDVMKALYYLMEQTYET
                     GQAVPVTGGQVMLA"
     assembly_gap    6930..7029
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7085..8344)
                     /locus_tag="PK576_05390"
     CDS             complement(7085..8344)
                     /locus_tag="PK576_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE61061.1"
                     /translation="MNSRAYALTAPMPFSLFLALKYLKPKRSFTSVVTLISVIGVVLG
                     VAIIIIVRAVMTGFGDMWKEKILDFKPHITILSRNGGVIEGEGEICRRLEQVPGVTAV
                     SPGLETRVLVEHRRRVVAPLLIGTDPARAQHVMKLDRLAAGAFELEGDAVVLGIDLAA
                     ELGIRVGEDLLVYSPMNLVSRDELFFPERLTVTGIYNSGQRDFDSGFMITSLAVVRDL
                     MGMRSGVYSIHLKVADPQNTVGFSRLVSEIRALLPIYSVRTWQEIDSQLFNALAVEKN
                     MMVILLMFITVVAIFCVTNTLIVLTVQKTDEIGLLKALGFSSRQVMMAFVLHGWIQCL
                     IGTALGIGAALLILENLQGLVSLLGRFGLEVFPKDVYGLAQIPWRVIPREVTDVALSV
                     ILFCTVASFLPAWRAARMDPVTALRKE"
     gene            complement(8392..10104)
                     /locus_tag="PK576_05395"
     CDS             complement(8392..10104)
                     /locus_tag="PK576_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE61062.1"
                     /translation="MSSYLDMNEVNSRLGEIIEMYTEDSYLRRLKKLFSGLKAPRHSR
                     EYKMAMIELQRLSAPLTAILLPVFAVVMLSVMDGGATGPDRLIETQVLEAEEIKDLDK
                     FQEEFERPEEPMEEVDIDIPVTNPNMPVIPSDVPTDQPVSPQPQAFDAVMMVKSPVIL
                     KNIYGTTRSTGTRGTQLARFGGDAFTEEAVMRALRWLKKNQQADGSWNRNKVAMTGLA
                     ILAFLSHGEKPGDSAEFGETVQKGLEYLMRVQNKKTGRLPGNYDHPIATYALCEAYGM
                     TINPNVKTAAEMAMVPIIKGQHPTGGWTYHMDPNPDKETGKYRDDTSYMGWCAQAIKA
                     AQLARLQVDGLEKASKLAIRGFKANAGPNGGFGYTGPGAGGLTSVGTLCMQLLGASNE
                     PEVRKSLDLMDSWVPTFSSYTILADELAKIEKSQLKQNQTPLSVAKARVIKTLPPEEQ
                     KFFNASPQYYFYYATQCKFHAGGKRWDSWNKQMKPNYVKAQQVKKDAIQDHKGTLRDI
                     GWWENTDAHSDRPVMDTCLAALQLMVYYRYLPTTSKEAVQVEAEISASTTDTGDIQVD
                     TGNL"
     gene            complement(10126..10533)
                     /locus_tag="PK576_05400"
     CDS             complement(10126..10533)
                     /locus_tag="PK576_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE61063.1"
                     /translation="MAYKRKKNKGESPKLDMTPMIDVVFQLLIFFVVTLKQEDILSKL
                     SAARPAANPDEKVKEQQQDLINVIVAPQGFVFNGRPMRLPELDRSIERLSGYSKTAMV
                     IIKCYADSTHAMLIQVLDVCNKHGMTNLSIFSM"
     gene            complement(10551..10979)
                     /locus_tag="PK576_05405"
     CDS             complement(10551..10979)
                     /locus_tag="PK576_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE61064.1"
                     /translation="MAKEYEQCEIDMTPMIDVVFQLIIFFVVTMKMNQEFNQDIVLED
                     GKHGVTITAENMPPTTLEIELDRRGRVSIHNATLTEKNLRDILRARVNKHGNEFPVLI
                     RADRRTLHDKVRKLMDVCTSCGVWKLSFVAVQEHKARDDR"
     gene            complement(11133..11933)
                     /locus_tag="PK576_05410"
     CDS             complement(11133..11933)
                     /locus_tag="PK576_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE61065.1"
                     /translation="MMKRLVMSAVLVAGLMMGASGVSAQDAAPAAAAAAAPAAVENAQ
                     PTESAKKSSKDSTGFLAVVLGSGWLGAVLWLALFGCGAAAIYFIVDCSILIRPQKIMP
                     QALIAKVKGAMAEGDVMKALHACESEPGPMANILSAGFSHVEEGFEVIQESIGAAADL
                     ETERIMQRLTWISVCSNLSPMLGLLGTVQGMIMCFGTLATGAPDVAALALAIAQALWT
                     TAGGLAIAIPAITCYYSIRNNANRLILRMQAMTMELIKDLRNVEVVSD"
     gene            complement(12011..12817)
                     /locus_tag="PK576_05415"
     CDS             complement(12011..12817)
                     /locus_tag="PK576_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61066.1"
                     /translation="MKMSTQTGRVAVALAMLTAGTAAAQIAGVITTSEGRKIEGVIKW
                     KVREKAYAITAKGGNVELEMSPANISDVQIPRPKELDAAIANMNQGNAAAAIPVLEKL
                     ANDYLMLQWDKPATRLLAEAYVASGNADKARQVCEKVIAANPEAAYLGEMAPAYWKAL
                     LKSGRVTKVEDLMAKAIKSGDRAASASALIMRGDVILAGGETNENAKKALRDGFLRVI
                     TLYKSEKDVQPEALYKAAKCFDNLGQSSRADQMRTALKSEYGSSEWAHKR"
     gene            complement(12844..15849)
                     /locus_tag="PK576_05420"
     CDS             complement(12844..15849)
                     /locus_tag="PK576_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61067.1"
                     /translation="MISGHGLALLWCAIVLSAFLSRAQGDVDNQELREELKYIDALQQ
                     LQMPDLAAEVIAEAKKRFPEAAAQFKIREFQGLLWQGKFDEVRKVVEALPDKNGEEYW
                     ALNLAMADAYYAFGKYMDADKLYLAFFKAFSKPPAALTSFYRDSAYKHAQMLIRLGKQ
                     TEALAAFKRVFNVKLEEEVERQVLADMAELMLKIAPNLSKKGEKDAMLKEAEAAVDKL
                     LWKQDVWFGKAIVMKAHIFLLRGDVKGAQDLVENYMPQLRTIHDSLVEQDADGSLGML
                     RMSPMPQCRYLLAVLLMNEAVAEAQKEGGSDDRIKDLLLGERDPKTRQRKGNGAFNHF
                     INVFIRYPESQWAAEAGERSEALRKLIKERYNTDIRTPVTVEQMAKVRQMQFAGARLL
                     FSQSQFKEAIDKYLLVLNQFPETPESVPALGDLAISYIETAGKDPDALLMADTVTGHL
                     SERFCAKEEMMKEAGDQVRRIGDRFGEMAMEDKKRETYALFFRDYPTHYAAGQLVMSF
                     GEREFHAKNYTGALTYYQLITDQYQHSTYYFDALNRIAQVYREEGDRTNEIQALEVYV
                     SRLAERDRPGNPLVVGKFRLADAQREYGNALMRAAVTNEQAEATQKEAVLWLTRAAVG
                     FDGVAKLLREKAGEYQNNAEEKQRNAQILEMSVFTKAVCLAQVNFPPERLTQFRKLAI
                     AAFEEYVKQYPEGKLAPRAQLQIGTLHTILEDASNAQAAFEKLSKQYPQSEEARNSVP
                     LLAASLIEMGLRGEGVAKYREMFGAGGKYTEGQFMAAAQALEQAREYDMALQAYDRVL
                     ATAKDLPVVANAKLGRARVLVGQKKYQEAHKALDAFIQDKALSNLEIVVDANMLLVEV
                     ASEEGRHEKDDNERTRLFNTAIDALKMVKKYRKEPEEMVELDLAAGQILLRKMEAEKK
                     LGLKEQVAETRGKAIVAFQVMIMNINPGNAKLASTLEKAYFYCVPLLLEHKKFKDAEE
                     DCETYLRIFPNGRYATDIRNWLNQAKIGQ"
     gene            16081..17214
                     /locus_tag="PK576_05425"
     CDS             16081..17214
                     /locus_tag="PK576_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61068.1"
                     /translation="MGKLLRVLVVVILLLSIVSLVFANLLFGKRELLIQRNSALEEHV
                     IRLSKTLEASDPQDVAAPDVQKDNSDVTDREIANPDRVSVLENYPIKLEQQNLPTLDF
                     GSTEKRIQLRSYYALTSEGKIDPDPVDQRPRTKGPDTMQELLDLAFERAKVQQATLNK
                     TRAELTKMRERVTGVVEEVNKLKAEGRTVKRDLKGAREQVAALETEKGELNGRITKLT
                     AEKRELAAEAADAKDQAERLNEEKLTLTEDLTKTREALEEMKKRFAGAGSRPVQESSG
                     VMVTALSAGDKGKILEANDELKFAILEFTDDAMTEMLGPERQNAMPQFEMNVRRIGRQ
                     SASGEFVTRIKLRQAVRGKNLVVADILSDWQQAPVEKGDVVFF"
     gene            17198..17380
                     /locus_tag="PK576_05430"
     CDS             17198..17380
                     /locus_tag="PK576_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61069.1"
                     /translation="MSFSSKAVRASLGLALLPTLALLLSGCMSDQPTETDMPWAAPAA
                     WEGTMPLPGGFMNRYE"
BASE COUNT         3345 a         5546 c         5022 g         3541 t
ORIGIN      
        1 ggccgcgaac tcgcgcctgg gaatcgcggg caggccaagc acatattcgc ccgcaggcac
       61 atctttggcg agcccgctct tgggcccgac ctgcccacgg tcgccgattt ccagatgccc
      121 ggagatgccg gcctgggccc agatcaggca ccccgaaccg atccgcgtgc tgcctgcgac
      181 accggcctgc gcgatgatcc cgctgcagga accgacctgc acgttgtggc cgatctgcac
      241 aagattgtcg attttggtgc tgttgccgat ccgcgtgcgg ccgaaccgcg cgcggtcaat
      301 ggtggcgttg cacccgacct cgacatcatc ccccaattcc acaatgccaa gttgggggat
      361 cttgtcgatg gtgaccgtgc cgtccgccgc caccttcggg gtgtagccgt atccatcccc
      421 gccgatacgc acgccggagt gcaggaccac acggcagcca aggatacaac cctcgcgcac
      481 cgtcacctgc ggataaatgt ggcatccctc acccatcgac acctgacgtc cgaggaacac
      541 ctgcgcctcg atcacgcagc cgtcgccgat gacacagccg tcttcgatca ccgtatacgg
      601 cccgacatga accgaggccc ccagatgcac atcacgtccg atcagcgcgg tgggatggat
      661 gcccggagcg cgttcgggcg ggctcggcgc gaaccagacc gcggcgcggg cgaaggcgcc
      721 atcgggatcc gggacacgga tcaccgcagg cgcgggacag gtgccctgcc acgtctcgga
      781 gaccaacacc acgctcgcct tggtggcggc gagctgtgcc gcgtacttcg cgtccttgaa
      841 gaacgcagcg tcgcccgggc gggccgcgaa gaggctctcc agcgcacgca cctccacgct
      901 gccgtccccc gtcagcgtcc cgccgagacg ttcggccagt tctatcgccg tcaggatcat
      961 ttcgtcttct cggctgcagc cttctccttc tcggcctttt ccttggcctc ctgacgcacc
     1021 ttgggatcga cgcccatccg tttgagcacc tcatcggtga catccagttt ggggtcgaaa
     1081 taggcgatcg tggtgccgtc gagcacgacc agatacttct tttcctgcgc gaacttggag
     1141 agaacctcgc ggatgtcgct cgtgacctgc cgcaacaggc gggtgtcgag atcacccaag
     1201 tcacgctgca gtttctgcat ctcctggcgc agatcgcggt ccgcttcggc gagcacgtcc
     1261 cggtgcttca tggccttatc ctccgcctcg gcacgcgcct tgtcgttgag cgccggattg
     1321 cgggcctcct tcaccgcctt ttcgtaatca tcacggagct gctcgaagcg gtcgcgctgc
     1381 ttgtcgagct tgctctggaa ctccttctcg gtgtcctgca tcaacttgcg gtcccgctcg
     1441 cggctcggat ggaaccgtac gagatcgacc atgttgacca ctgcggtgcc gccctgggcc
     1501 atcgccatcg tgacggcgaa aaccgccgcc agtatgccgc tcatctgttt cataccattc
     1561 ctttattcga acccgatcgc gaacgagaag aactcgtcat ccgtgtaact gtcatcctcc
     1621 accacgggct tggcgaagtc aaaccggatg gggaagccgg ggatatcgat ccgcaggccc
     1681 acacccaccg aaacacaaac gtcggaaagc tccggatcga acacatcctc gccgatcgac
     1741 ccgacatcca tgaaggtcgc cacgcgcacc gccttgaaga ccggcaccgt gtattcgacc
     1801 gtggccatgg ccagagtctg gccaccgatc ggcgcgtgct tcttgctgcc ttcgccgcga
     1861 tagactttgg ggccgatatc gcggtattca acgccgcgca ccgtgcgcgg gccacccaga
     1921 aagagtttct catagatcgg cacatcgccg ctgtaggcat ccactgtttc gaagcggccg
     1981 cgcaggctca gcacatgctt gtaccaaggc atgcggaacc agtggcggta attcacgccg
     2041 agtttgtaaa tctcgttgtc gccgatcgca ccttcggaga gttcgccgaa cagggtggtc
     2101 tggtatccct tggtgggcac gaacggctgg tcgcgcgtat cgtgggccca atagaggcgg
     2161 atcacactgt tgagcgcgtc gccgtactcg tcctcctgcc agttgaaata atcgttctcg
     2221 tcgccgttcc agttttccgg cgtggaagcg gtgtcgccct tcgggtccag ataccactcg
     2281 cccgattcga cgtcatccat ctccaccagt tccattgtgt agcgcagacc cacgcggccg
     2341 actttgaccg gataggagag gccgaccgag ccgccggtac ggatctcgtc gtattcgtca
     2401 aaccagcgca tccggcggta agcctcgacc gtcagcgcca gcgggcggtc caggaaccag
     2461 ggttccgtcc aagagacctc ggccgtctgc cgacgcgaac cgacttcaat gcccaagcgc
     2521 gccttctgac cgccgccggt gaagttcggc cagttgagga tgtcgaagtt gctttgggtg
     2581 atctccatga agcccacgag attatcgaca ctcgaaaaac cggcgccgat catgaagttt
     2641 cctgtgcgct gctcttccac ctcatagacc agatcgcgcg tgccgggctg tccggtttgt
     2701 tccgtgtaat ggcgcacgtt ggcgaaatag ccgaggtttt tcaggcggtt ctcgctgcgc
     2761 tcgatgcgcg gctcgttcat gatctcaccg gggttgacca gcacctcgcg gcgaatgacc
     2821 ttgtcccggg tcttgttgtt gccgcggatc acgatgttgc ggatgtagac caattcgccc
     2881 tcgcgcacat caaacgcgat cgacatgcgt ccgggtttgc ccggcaccgt ttccttcatg
     2941 ggccgcacga tcgtgtccac atacccgcgc gagccgtaat agtcgcggat atccttggcc
     3001 gcgtcatcaa tcgccttacg gctcgccaag tcttccgcct tcagccggga ggaggcgagc
     3061 acctcgcgtt ccggaaagag tttcacgccg cggatcgcgg tggattccac atggtacacg
     3121 tccccttcgg tcacggcgaa gaccacatcc accttctctt tgccgacctt ctccatcacc
     3181 ggcgaagcca ctttggcgtc gaggaagccg tgattttgat agacctcctc ggcctgttgg
     3241 cgcgcgtcct caaggtcctg ccgcgaaacc ggcgtgtcgg tgaaccagcc gcgcgggtcg
     3301 taccacggcc gctgaccaaa cgaggaacgc aggtccgcct ccggaatgga ggtgtttccg
     3361 gtgaaacggt acgcgcggat cttggcgcgc tcgccctctt cgatggtcag ggtgacattc
     3421 gcaaaaccgg cgctgccctg aatgggggtg atggccatgc cgattttgac atccggaaag
     3481 tacctcttca catactcgtc acggactttg gcaacctggg tcgacaaaac cggttcatca
     3541 atgtaatcgc cgctcttgag ttccgtgagt ttgcggattt tactctcgct taaatagttc
     3601 gcgccctgaa tggccagcgg ctcctgaaac cggtaccggc ggcgcaccac gtagatcacg
     3661 cggattccgc cctcgatttt ctccaactcc acacccgcgt acccgtaacg tccggtatcc
     3721 aacaacgcgc gcacatcttt ggagagcgcc tcctgcgaga gcttcgagcc tgcggcaacc
     3781 ccgcagaccg acagcacatc tgaagcgtcc gtcccgagtt ggtcgagcac acggacctga
     3841 acctctttca ccgtgttatc ggccgccaac acccaccctg cggccaagac cgccaccgcc
     3901 aaccacgcgc gcatcacttt acccattttc gcaactcctc atcccattca accaaacgtc
     3961 cagccccgcc gcaacggcgg actgccacca taccggaggc atgaccgcag aaaatccggt
     4021 tcatatgcga attggaaaaa ttaaacctga tcctgcggca aaacacaagc ctgttgtcgt
     4081 cggttttggc cggatggccg ggggagcgcg aaatgttgac ccgcgacggg cggcctgcgg
     4141 tgtcgggaac gcaggatggg gagggcgcga attcatccgt gcattggtgc ggaagccgga
     4201 aatgcgggca tccccgcccg ctggcggatc agtcggatca gtgctctgcc aaatcaccca
     4261 aacgccgcag gccgaatgtc gcgggtcgca agtcaacgta cacgaaaacg aaggcgcgaa
     4321 cgaggacggg gacttggggt tgactttcca aacgtatccc gcccataatg tgcctcaaat
     4381 gcagacaccg ggggaggggt ctgcggtctc agaggaaagg tgcgtgatga aaaaaaagac
     4441 gcttgcggtc gttgtctgtc tgttttgctc aattctgtca ccggtgtatg cccgactcgt
     4501 cgacaccgtc ccgatgattg tccgcgcgga cagcgaggcc accgtgaccc tcacctttgc
     4561 ggatgagccg ctgctcgcca aaccggacga tgtgcaggtc gaatacatct gcgcggacgg
     4621 tctggactcc gcaggcgtac cgctcggata cggctgtttc cagccggtcg cgtccgttgt
     4681 ccgcgacaac acgctcaccg tttccgtgcg gttccgcgga gagaccgcgc acaccttgcg
     4741 cgtgatccag aaaagctccg atccgaaaaa accgaacgtg ctggggatcg ccaggcttta
     4801 ctcgttgtgc ccggatttct ttgccctgcg gccttaccgg ggaaacatgc acatgcacag
     4861 caagttttca gatggcaaca agaacgagtc tcccgcactg atggtcgcca catgccgtaa
     4921 gctcgggcac gacttcgcga ttgagaccga tcaccgctct tatgcgggat cgctggatgc
     4981 catcgaagcg ttttccaaat tgcccacgga catgaagacc ttccccggcg aagaggtaca
     5041 cagccccggg aacaacgtgc acatcctctc gctcggtgct tcggaaagca tcacggattg
     5101 gttcacgaag tcaaagaccg aatacgatca ggcggtcgcg gcggaacagg ccaagctgcc
     5161 ggacacgatt cccgatcgtt tcaagtatcc gatcgccgcc tcgttcgcgg tttgggacaa
     5221 aatccgcgcc tgcggcggca tcgccgtgtt ttgccacccc tattggcgac cggcctaccg
     5281 gcagtacatc ccggcggtgg tttcggacta tctgctgcag accgcgaagt tcgacgccgt
     5341 ggaggtcctg aacacggaca gcagtgagtt gggaatcctc cactaccacg aactgcgcgc
     5401 gcaagggctg aagatcgccg gtatcggtgt gactgacgcc cactcgtcaa aacatctgga
     5461 acccgcctac acgctgattt tggcggaaac actcgatttt ccgtcgctcg ccaaaaacat
     5521 ccgcctgcgc aactgcgcgg cggtggatat cgatcccgtt tcgaaacggc aaaccgtcat
     5581 cggcgagttc cggttctccc ggtacgcgct cttcctgctc catcatttct atccgttgca
     5641 gaatgaggtc tgcaaaaggg agggcgaatg gctgctgaag gcgcttgaag gtgacgagca
     5701 ggccattgcc tcgttaaagg catcgcaggg aacgactccc gcgctcagga cgaaatattg
     5761 gcagaagtga acccgatatt gcgaaaggac cccctcatgt tgaccctgat tctccccgcc
     5821 ctgcggggcc tgatggcccg cggcgatacc ctgccgcgtc tggcgcgtgt ccagccggaa
     5881 ggcggcgagc cgctgctcac cgtgccgccg ggatgcgatg acgcgttcgc cgtgttgcaa
     5941 agccatgtgc cgccaggcac aagccgcgtc accctggaca ccggcggcac ctacgaggcg
     6001 gtctttactg atcttccggg tcgtgccgcg ggacggatcg cgctggtgac cggcgcggcc
     6061 caaggtttcg gatttgaaat cgcatgcgat cttgctgccc aaggtgcctg ggtcgccctg
     6121 gccgacatca acgaagcggg cgtgctcgcg gcggcggagc aggtcaatga ggcttgcgga
     6181 cgttcggcgg ccgtcggtgt ggccgtgaac gtggcggatc ccgattcggc gcgcgcggcg
     6241 gtggcagcgg cggtgacggc attcggcgga atcgacctgc tggtctcaaa cgccggcgta
     6301 ctgcgggccg gcagcgtcaa gacgcttccc gtcaaggacc tccgcttcgt gacggatgtc
     6361 aattaccacg gttatttcct catggttcag gcggtggcac ccattctggc gctacagcac
     6421 cgggccgtgc cgtcacgctg gtttgacatc attcagatca acagcaaatc cggactgacc
     6481 ggttcgaaca agaacggcgc gtacgcggga tcgaagttcg gcggcattgg cctgacgcag
     6541 agtttcgcgc tggaactggt ggaggacggc atcaaggtca actccatctg ccccggcaac
     6601 ttctatgacg gaccgctctg gtctgacccg gagaaaggtc tgttcaagca gtacctcgac
     6661 agcggcaagg ttccgggcgc gaaaacattc gaggatgtca aacggttcta cgagtccaag
     6721 gtcccgatgc ggcgcgggtg taccacgccg gacgtgatga aggcgctgta ctatttgatg
     6781 gaacagacgt acgagacggg acaggccgta ccggtcacgg gtggccaggt gatgctcgcc
     6841 taggccgagg cggtcggaac cagcagaccg tcgcgcaact ccagcacacg gtcgcagagc
     6901 gccgcggtct cgggtgaatg ggtcaccatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnng gggaagcgtg taggtcttga acaacactgt ggcttctaac atcacggcct
     7081 ttcttcactc tttacgcagc gcggtcaccg gatccatgcg cgcggcgcgc caggcaggga
     7141 ggaaactcgc caccgtgcag aaaaggatca ccgagagggc cacgtcggtc acctcgcgcg
     7201 gaatcacgcg ccaagggatc tgcgccagcc cgtacacgtc tttggggaac acctccagcc
     7261 cgaagcggcc gaggagcgac accagaccct gcaagttttc cagaatcagc agggccgcgc
     7321 caatgccaag cgccgtgccg atcaggcatt ggatccagcc gtgcagcaca aaggccatca
     7381 tcacctggcg cgaggaaaat ccgagcgcct tgagcaggcc gatctcatcg gtcttctgca
     7441 cagtcagcac gatcagcgtg ttggtcacgc agaagatcgc caccacggtg atgaacatca
     7501 gcaggatcac catcatgttt ttctcgaccg ccagcgcatt gaaaagctgg ctgtcgatct
     7561 cctgccaggt ccgcacactg tagatcggca gcagcgcgcg gatttcgctc accagccggc
     7621 tgaaccccac ggtgttttgg gggtccgcga ctttgaggtg gatcgaatag accccggatc
     7681 tcatccccat caggtcgcgc accactgcca gcgaggtgat catgaatccc gaatcgaaat
     7741 cccgctggcc cgagttgtag atccccgtaa cggtcagtcg ttccggaaaa aagagttcat
     7801 cccgggagac gagattcatc ggcgagtaga ccagcaggtc ttcacccacc cggattccca
     7861 attcagcggc cagatcgatc cccagcacca ccgcatcgcc ttcaagttcg aacgcgccgg
     7921 ccgccaagcg atccagcttc atcacatgtt gcgcccgggc gggatccgta ccgataagga
     7981 gcggagccac cacacggcgg cggtgttcga ccagcacccg cgtctcgagt cccggcgaga
     8041 ctgccgtgac ccccggaacc tgctccagcc tacggcaaat ctccccttct ccttcgatca
     8101 ccccgccgtt ccgggacaag atggtgatgt ggggcttgaa gtcgaggatc ttctccttcc
     8161 acatgtctcc gaaaccggtc atcaccgcgc ggacgatgat gatgatcgcc acgcccagca
     8221 ccacaccgat cacggaaatc aatgtcacca ccgaagtgaa tgaacgcttc ggcttgaggt
     8281 atttcaacgc gagaaagaga gagaagggca tgggggctgt cagcgcatac gcgcgcgagt
     8341 tcatgagtgc atgagttcgc ggcaggaaga aaaaacgaac gcacgaatga actacaagtt
     8401 tccggtgtcg acttggatgt cccccgtgtc cgtggtggag gcgctgattt ccgcttcaac
     8461 ctgaaccgcc tctttactgg tggtgggcag atagcggtaa tagaccatga gctgcaacgc
     8521 agcgaggcag gtatccatca cgggacggtc cgagtgcgca tcggtgtttt cccaccagcc
     8581 gatatcccgc aaggtgccct tgtgatcctg gatggcatcc ttcttcacct gttgggcctt
     8641 gacgtaattg ggcttcatct gcttgttcca actgtcccag cgcttgccgc ccgcatggaa
     8701 tttgcactgg gtggcatagt agaaatagta ctgcggcgac gcattaaaga acttctgctc
     8761 ctcgggcggg agggtcttta tgacacgggc cttggccacg gacaacggcg tctgattctg
     8821 cttgagttgc gatttctcga ttttggcgag ttcgtcggca aggatggtgt agctggagaa
     8881 ggtcggcacc cacgaatcca tcagatccaa ggacttccgc acctcgggtt cgttcgaggc
     8941 acccagcaac tgcatacaga gcgtgccaac gctggtcagg ccgccagcgc cgggaccagt
     9001 gtaaccgaac ccgccgttgg gcccggcgtt ggccttgaag cctcggatcg ccagcttgct
     9061 ggctttttcc aggccatcca cctgaagacg cgccagttgg gcggccttga tcgcctgcgc
     9121 gcaccatccc atgtaagaag tgtcatcacg gtatttgccg gtctccttat ccggattggg
     9181 atccatatga taagtccaac cgcccgtggg atgctgcccc ttgatgatcg ggaccatcgc
     9241 catttccgcg gccgtcttga cattggggtt gatggtcatg ccgtaggctt cgcaaagcgc
     9301 gtacgtggcg atcgggtggt catagttgcc gggcaggcgg ccggttttct tgttctggac
     9361 gcgcatcaga tactccagcc ccttctggac cgtctcgcca aactcggcgg agtcgccggg
     9421 cttctcgccg tgcgacagaa acgccaggat ggcgagaccg gtcatggcga ccttgttccg
     9481 gttccacgaa ccgtccgcct gctggttctt tttgagccag cggagcgcgc gcatcaccgc
     9541 ctcttccgta aacgcatcgc cgccgaagcg cgcgagttgc gtgccacgcg tacccgtgct
     9601 ccgggtggtg ccgtagatgt ttttgagaat cacggggctt ttgaccatca tcaccgcgtc
     9661 gaaggcctgc ggctgggggc tcaccggttg gtctgtcggc acgtcggagg ggatcacagg
     9721 catattcgga ttggtcacgg ggatatcgat gtcgacctcc tccatcggct cttccggcct
     9781 ttcaaattct tcttggaact tatcgaggtc tttgatctcc tccgcttcca gcacctgcgt
     9841 ctcgatcagg cggtcgggcc cggtcgcacc cccgtccatc accgagagca tcacaaccgc
     9901 gaacacgggc agcagaatgg cggtgagcgg cgcggagagg cgctgcagct cgatcatcgc
     9961 catcttgtac tccctggagt ggcgcggcgc cttgagcccg ctgaacagct tcttcaggcg
    10021 gcggaggtag gaatcttccg tatacatctc gataatctct ccgagacggg agttcacctc
    10081 attcatgtcc agatagctac tcatgtcaga ctccttatca catcatcaca tactgaaaat
    10141 cgacaggttg gtcatgccat gcttgttaca gacatccagc acctgaatca gcatggcgtg
    10201 ggtggagtcg gcgtaacact tgatgatgac cattgcggtt ttgctgtatc ccgacaacct
    10261 ctcgatgctc cggtccagtt ccggcagccg catggggcgg ccgttgaaca caaagccctg
    10321 cggcgccacg atcacgttga tcagatcctg ctgctgctcc ttaacctttt cgtccgggtt
    10381 cgccgcgggg cgggccgccg agagtttcga gaggatgtcc tcctgcttca gcgtgacgac
    10441 gaagaagatg aggagttgga agaccacgtc gatcatcggg gtcatgtcca gtttcgggct
    10501 ttcgcctttg ttttttttcc gtttgtatgc cataggaaaa actcctgtcg ctaccggtcg
    10561 tcccgggcct tgtgttcctg aaccgccacg aacgaaagct tccacacgcc gcaggaagtg
    10621 cagacatcca tgagtttgcg gactttatca tgcaacgtac gccggtcggc acgaatcaac
    10681 accggaaact cgttgccgtg tttattgaca cgcgccctca ggatgtcacg caagttcttc
    10741 tctgtcaacg tggcgttatg gatcgagaca cgcccgcggc gatccagttc gatctccagc
    10801 gtggtcggag gcatgttctc ggccgtgatg gtgacaccat gcttgccgtc ctccaaaaca
    10861 atatcctgat tgaattcctg attcatcttc atcgtcacca caaagaagat gatcaactgg
    10921 aacacgacgt cgatcattgg cgtcatgtcg atttcgcatt gttcgtattc tttggccatt
    10981 tttcaagatc cgatttgcgg ggtaagccgc acgtgtttcc aactcgccac aggaccggaa
    11041 agatgaccgg ccatcccgcc gcagaaagat cggcaaaagc cgtcctcctc tagccgtaca
    11101 gaaaatccgg acggctaagg atgacacgga cattaatcgc tcacaacctc gacgttacgc
    11161 aagtctttga tcaactccat ggtcatcgcc tgcatgcgca gaatcagccg gttcgcattg
    11221 ttacggatac tgtagtaaca ggtgatggcg ggaatggcaa tcgccagacc gcccgccgtc
    11281 gtccaaagag cctgggcaat ggcgagagcc agcgcggcaa cgtccggagc acccgtggcc
    11341 aacgtgccga agcacatgat catgccctgc accgttccga gcaacccgag catcggcgag
    11401 aggttcgagc acacggagat ccaggtgagc cgctgcatga tgcgctcggt ctccaggtcc
    11461 gcggccgcgc cgatcgactc ttggatcacc tcaaagccct cctccacatg ggagaaccct
    11521 gcggagagga tgttggccat ggggccgggc tcgctctcgc aagcgtgcag cgccttcatc
    11581 acatcaccct cagccatggc gcccttaacc tttgcgatca gtgcctgcgg catgattttc
    11641 tgcgggcgga tcaggatgga gcagtcgacg atgaaataga tcgccgccgc gccgcacccg
    11701 aagagcgcga gccacaggac ggcacccaac caaccggatc ccaagaccac cgcaagaaat
    11761 ccggtgctgt ccttggatga ttttttcgcg ctctcggtcg gctgcgcgtt ttccaccgcc
    11821 gccggggccg cagccgccgc agccgccggg gccgcatcct gagccgacac tccggaggcg
    11881 cccatcatca aacctgcgac caacactgct gacatgacca gtcttttcat cattaacctc
    11941 tgcttgtttc ccttactgtt ctaccattcc acccgcaccc ctccggcacg ggaaatcccg
    12001 gacgcggccc ctaccgtttg tgcgcccact ccgatgagcc gtactcgctc ttcagcgcgg
    12061 tccgcatttg atccgcgcgc gaggactgcc cgaggttatc aaaacatttg gcggccttgt
    12121 acagcgcttc aggctgcacg tccttttcac tcttatagag ggtgatcacg cgcagaaatc
    12181 catcgcgtag cgcctttttg gcgttctcgt tggtttcgcc cccggccaga atgacatcgc
    12241 cgcgcatgat cagcgcgctc gccgaagccg cccggtcgcc gctcttgatc gccttggcca
    12301 tcaggtcctc gactttggtg acgcggcccg atttcagaag cgccttccag taggcgggcg
    12361 ccatctcgcc cagatacgcg gcctcgggat tggcggcaat gaccttctcg cacacctggc
    12421 gggccttgtc ggcgttgccg gatgccacgt acgcctcggc cagcagacgg gtggccggtt
    12481 tgtcccactg gagcatcagg taatcattgg ccagtttctc cagcaccggg atcgccgccg
    12541 ccgcattccc ctgattcatg ttggcgatcg ccgcgtccaa ctctttgggc cgcgggattt
    12601 gcacatcgct gatgttggcg ggggacatct ccaactcgac gttcccgcct ttcgcggtga
    12661 tcgcataggc cttctcgcgg actttccact tgatcacacc ctcgattttc cggccttcgg
    12721 aggtggtgat cacgcccgcg atctgtgccg cggcggttcc cgcggtcagc atcgccaggg
    12781 cgaccgcaac ccttccggtc tgtgtgctca tcttcattcg tttgctcctt aaagaacgtt
    12841 gcctcactgt ccgattttcg cctggttgag ccagttgcgg atgtcggtgg cgtagcgccc
    12901 gttggggaag atgcgcaggt aggtctcgca gtcctcctcg gcatccttga acttcttatg
    12961 ctcgagcagc agcggcacgc agtagaaata ggccttctcc agcgttgacg ccaatttggc
    13021 gttgccgggg ttgatgttca tgatcatcac ctgaaaggcc acgatcgcct tgccgcgcgt
    13081 ctccgccacc tgctccttga gcccgagctt cttctccgcc tccatcttgc gcaacaggat
    13141 ttgacctgcg gcgaggtcca actcgaccat ctcctccggc tctttgcggt atttcttgac
    13201 catcttgagc gcatcgatcg ccgtgttgaa cagacgggtg cgctcgttgt cgtccttctc
    13261 gtgccttccc tcttcgctgg ccacctcgac cagcaacatg ttggcgtcca ccacgatttc
    13321 cagattggag agcgctttat cctgaatgaa cgcgtccagc gccttatggg cctcctgata
    13381 cttcttctgc ccaaccagca cgcgggcacg cccgagtttg gcgttggcca ccaccggtag
    13441 gtctttggcc gtggcgagca cccggtcata ggcctgcagg gccatgtcat actcccgggc
    13501 ctgctcgagc gcctgcgccg cggccatgaa ctgcccctcg gtgtactttc cgcccgcgcc
    13561 gaacatctca cggtacttgg ccacaccctc accgcgcagg cccatctcga tcagactggc
    13621 ggccagcaac ggcaccgaat tcctggcctc ctcggactgc gggtactgct tgctgagttt
    13681 ctcgaaagcg gcctgcgcgt tggacgcatc ctccagaatg gtatggaggg tgccgatctg
    13741 caactgcgcc ctgggggcca gtttgccctc cggatactgt ttgacatact cctcgaaggc
    13801 ggcaatggcc agcttgcgga actgcgtcag gcgctccgga gggaagttga cctgcgccag
    13861 acaaaccgcc ttggtgaaga ccgacatctc gagtatctgc gcgttgcgct gtttctcttc
    13921 ggcattgttc tgatattcgc cggccttttc gcgcaacagc ttggcgaccc cgtcaaaccc
    13981 caccgcggcg cgggtcagcc agagcacagc ctccttctgg gtcgcctcgg cctgctcatt
    14041 ggtgacggcg gcgcgcatca gcgcgttgcc gtactcgcgc tgggcgtccg ccaaacggaa
    14101 cttgccgacc accagcggat tgcccggccg gtcgcgctcg gcaagccggg agacgtacac
    14161 ttcgagcgcc tggatctcat tggtccgatc cccctcttcg cgataaacct gggcgatgcg
    14221 gttcagggcg tcgaagtagt aggtcgagtg ctgatactga tccgtgatga gctggtagta
    14281 cgtgagggcg ccagtgtaat tcttggcgtg gaactcgcgc tcgccgaaac tcatcaccag
    14341 ctggcctgcc gcgtaatggg tggggtagtc gcggaaaaag agcgcatagg tctcacgctt
    14401 cttgtcctcc atcgccattt cgccgaagcg gtcgccaatg cggcgcacct gatcccccgc
    14461 ctccttcatc atctcttcct tggcgcagaa gcgctccgag aggtggccgg tcaccgtgtc
    14521 cgccatcagc agcgcgtccg gatccttccc cgcagtctcg atataactga tggccagatc
    14581 gcccagcgcc ggcacggact cgggggtctc ggggaactgg ttgaggacca gcaggtactt
    14641 atcgatggcc tctttaaact ggctctggct gaaaagcaac cgcgccccgg cgaattgcat
    14701 ctggcgcacc ttggccatct gctcgaccgt caccggcgtg cggatatccg tattgtaccg
    14761 ctctttaatc agcttgcgga gggcttccga gcgctcaccg gcctcggccg cccactggct
    14821 ctccggatac cggatgaaca cattgatgaa gtggttgaag gcgccgttgc ctttgcgctg
    14881 gcgcgtcttg gggtcgcgct cgcccagcaa caggtctttg atgcggtcgt cgctgccgcc
    14941 ctctttttgg gcctccgcca cagcctcgtt catcagcagc accgccagca gataccggca
    15001 ctgcggcatc gggctcatgc ggagcatgcc cagcgacccg tccgcatcct gctcgaccag
    15061 ggagtcgtgg atggtgcgaa gctggggcat gtagttttcc accagatcct gcgccccctt
    15121 gacatccccc cgcaggagga agatgtgcgc tttcatcaca atcgccttgc cgaaccacac
    15181 atcctgcttc cagagcagtt tgtcaaccgc ggcttcggcc tctttgagca tggcgtcctt
    15241 ctcgcccttt ttggacagat tcggcgcgat cttgagcatc agttccgcca tgtccgccag
    15301 aacctgccgc tcgacctcct cttccaactt cacgttgaac acccttttga atgcggccaa
    15361 ggcctcagtc tgcttgccca gacggatgag catctgcgcg tgcttgtaag cggagtcacg
    15421 gtaaaaagag gtcagcgcgg ccggcggctt gctgaaggct ttgaagaagg cgaggtaaag
    15481 cttgtccgca tccatatact tgccaaaggc gtaataggca tccgccatgg ccagattgag
    15541 cgcccagtac tcctcgccgt tcttgtccgg aagggcttcc acgactttcc gcacctcgtc
    15601 gaacttcccc tgccagagca atccctgaaa ctcccggatc ttaaactggg cggccgcttc
    15661 gggaaaacgc tttttggctt cggcgatcac ttccgcagcc agatcaggca tttgcaactg
    15721 ctgcagggcg tcaatgtact tcaactcctc ccgcaactcc tgattgtcca catccccttg
    15781 cgcccgtgac aggaacgcgg acaggacaat cgcacaccac aacagcgcga gcccatgacc
    15841 cgaaatcatg cgaaagcact tctcattcat aatgcccatg ttatacggca atttggtttc
    15901 ccggtcaatt tcaaagaggc ggtccgtgag aaaaataatg cgcggccgcc ccaccctcaa
    15961 atcggcccgc cggaaacaaa aaagagtctt gttcattttg ttgttgcagt cgcgccccgt
    16021 tttgctttaa ttttgactct atggccgatc gtgtcacaag caaaggttga ggttctaaaa
    16081 atgggtaagc tgctgcgtgt attggttgtc gtgatcctgc tcctgagtat cgtctcgctg
    16141 gttttcgcaa acctgctgtt cggtaagcgc gagctgctga tccaacggaa ttctgctctg
    16201 gaggagcatg tcatccgcct gtcgaagacg ctcgaggcgt ccgaccctca ggatgtggcg
    16261 gccccggatg tccagaagga taactcggac gtcacggacc gcgaaatcgc gaatccggac
    16321 cgggtctcgg ttctggagaa ctatccgatc aagctcgaac agcagaatct gcccaccctg
    16381 gatttcggca gcacggaaaa acggattcag ctccggagct attacgccct gacctcggag
    16441 ggtaaaatcg acccggaccc ggtggaccag aggccccgca ccaaaggacc ggacacgatg
    16501 caggaactgc tcgacctggc gtttgagcgc gccaaggtcc agcaggccac gctgaacaag
    16561 acccgcgccg aactcaccaa aatgcgcgag cgggtcaccg gcgttgtcga ggaggtcaac
    16621 aagctgaagg ccgaagggcg tacagtcaaa cgcgacctga agggcgcccg cgaacaggtc
    16681 gccgcccttg aaaccgagaa gggggaactc aatggccgca tcaccaaact cacggccgaa
    16741 aagcgcgaac tggccgccga ggctgctgac gccaaggatc aggccgaacg cctgaatgag
    16801 gaaaagctca ccctcaccga ggatctcacc aagacgcgcg aggctctgga agagatgaag
    16861 aaacgcttcg cgggtgcggg cagccgcccg gttcaggaga gttccggcgt catggtgacc
    16921 gccctctccg ccggggacaa aggcaaaatc ctcgaggcca acgacgagtt gaagttcgcg
    16981 attctcgaat tcaccgatga cgccatgacc gagatgctcg gcccggaacg ccagaatgcg
    17041 atgccgcagt tcgaaatgaa tgtgcgccgc atcggacgcc agagcgcttc cggtgagttc
    17101 gtcacccgaa tcaagttgcg ccaagccgtg cggggcaaaa atctggtggt cgccgacatc
    17161 ctgagcgact ggcagcaagc cccggtggag aaaggcgatg tcgttttctt ctaaggccgt
    17221 acgggcgtct ctggggttgg ccctgctgcc gaccctcgcc ctgctgctct ccgggtgcat
    17281 gtccgaccag ccgaccgaaa cggacatgcc ttgggcggcc cccgccgcat gggagggcac
    17341 catgccgctc ccgggcgggt tcatgaaccg gtacgaataa aggcgaaggc cccttctgaa
    17401 tcagaggggg ccttcttgtt ttcttgatcg gctctcggct tgaggcattg ctgtcggttt
    17461 cgtcccgttc acgttcaggg aaacaggaat gcgtgcccct cccggttgtt ttccgaaaca
    17521 gcggttgacg ccgatgtata aacggcgtat gctt
//
LOCUS       DAOSLE010000055        17538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3450_length_17538_cov_7.914717, whole genome shotgun sequence.
ACCESSION   DAOSLE010000055 DAOSLE010000000
VERSION     DAOSLE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 17538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17538
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3450_length_17538_cov_7.914717"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..681)
                     /locus_tag="PK576_05435"
     CDS             complement(91..681)
                     /locus_tag="PK576_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase ThyX"
                     /protein_id="HOE61070.1"
                     /translation="MSQPPILKYFKTKSVWRDVADAARTTIRMEEGDREPSNAWKHRI
                     LLSEHSPIRQMLFKWKWVNLKYWVSVHFVRHKIGIEHFVSTQRSDRTGVNRDTMPQAS
                     LVDHECFANAQALIFISRKRLCKQASPETTEAWRLVLDAMREHEPELYRVCVPECVYR
                     GFCPEFKSCGYAQTEAFRQALDAYRSGNDANAARKD"
     gene            complement(720..1457)
                     /gene="fabG"
                     /locus_tag="PK576_05440"
     CDS             complement(720..1457)
                     /gene="fabG"
                     /locus_tag="PK576_05440"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOE61071.1"
                     /translation="MKTALITGAAKGIGRAIALGLAHDGFRIALHYHTSVEAAEETVA
                     AIRKAGGKAEPFAADLTVPAEVDELVKRVIGTCGGIHTLVSNAGILRSQLLAFTGLDT
                     WREVHAANLDAAFLLTKALTRPMARQQAGRIIYIASAAGLMGDLMRAAYSSSKAALFG
                     LAKTAARELAASGVTVNVVAPGIITTDMTAEIPETRRVKQLAAIPLGRFGTPDEVAGV
                     VRFLASEEAAWITGQILRVDGGLCPSP"
     gene            complement(1454..1912)
                     /locus_tag="PK576_05445"
     CDS             complement(1454..1912)
                     /locus_tag="PK576_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOE61072.1"
                     /translation="MTQILIPQLDANVVEVTLSRWRKQVGDTVEAGECVAELATDKAA
                     YELEAPASGTLLAVYATAKSVVPTGFIAGLIGAPGEEDPSIPEKNAALTAAYRGTASA
                     GSGTDRGTRIRATPRARRLAREHHIDLEKVRAETGADLIDETVLAPYLPA"
     gene            complement(1950..7709)
                     /locus_tag="PK576_05450"
     CDS             complement(1950..7709)
                     /locus_tag="PK576_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61073.1"
                     /translation="MNWTNVLIWAGLTWGCCAATAGLAAEEAWSTRLDLTERSEWGAL
                     NLTPERGALRLRLSHPDHTCQAEFTLSEGDTRFRLLPRGFFDDATERRVTTYPCPSLA
                     GPLHWITLKRHADVWILTVDDRPVARLPELWQGAVNVEHPPALQPPEAEADEYTQRLG
                     SFTFEDNFLVPAGSAFPPTWDIQSGIWRLHSVTGSISGSSGGYQLARQPKPEKSPNFY
                     TLEGGGTNAIVLAGEPFYSRYVFRAAVQHNTGTNGLVFLAGEHGGYFAFTARTDPVSD
                     RLILDLWQQPAAPNAPRLYLAGVQTELPAGQWLLLEVQTFDDRILCRADNIEVIRHKI
                     GLPPGGRFGLFANMPAGETTRFDDVTAASHQDRLFDSPEDVTFATRRQSPDVRSLQRH
                     DTAWLYFPPSATTTQMWAYGSSDDGPLRQEIRVVATTDSFLCGLTAGGSGDGAAPYRF
                     TCEQDGGGRTFRLEHVTTNGTVLLDSFQMPVSSNRVILALDALRPHELRAYADDLLVC
                     FHRPEDQPGGELGVLAVTPGDLFFTAPTVTSRETGLSERFEKNPLYVNDPFMRHWASP
                     EGQWITFKNGMTWFKGDITGAVRVRLPVVEEMELHLGVPEGLSNGLCRVSVKKGVIRV
                     FTPESGTNTAFEVSADQVPEVEFNNTKLRLFTLGLEGSVLWLGGDTLLLAQTHLHAPL
                     HGRRMRISGMTLDNLSRTLVKRDNVFDTLFTESLYNWTINGGRWEVINRFYCEPTWSH
                     MNGESADSLAALWSKYVFRGDFSIEFYAGMRMGWYARPGDLNLTMMSRSLSTGDGYTA
                     IATGWDPDHSQLYSRLIRNGSLLDLSTKYLVPRARTGLARQGYQPLVAGGRDIHGAWY
                     GMQLRRVGKTLHYIYDNEDVFRIEDPDPLQEGALGIWTYRNSMMVARIKIAAESIRPR
                     PFAFRALPPAAPPPTEPAAAPADTGIRINGRVAQPLTPDFWQAYDTVSHPNVRFRHLQ
                     TARPEMYVTSLLGAGSFLVRCMLPPAQPDKLLGWRFEIARHPQARVNFEFATVKEDGK
                     GGWTPIQHWTYVLCGSNESRGPRKICGALESLPASDPAGTNRVWTPVEIWVPSEIIRA
                     NQAVQIEGFGNLQPSDVQQGLEGNPPHAWYAIRHFREIHRGIPTVSGPPEKRAEIASL
                     TQLINGLSPGELQMVELPAALDSRRPVIEWAVPERAKFGLLAQSDPAVLGSIVVTPTH
                     PWPSPMLPPKQVQVDAQPAPFYTEGNTIRILVPLNILQPGRMTLALELYDGRFFRQVV
                     PMRKEDSVNSPPVLLSLTMPEGAVETFEARPFDTTPHKMAARVAVEVTDPRRGGVLAF
                     SNAGVYGRRLTGQLFPRFDIAATPLLQFRYKGDPMAIVSLAFGEFAFTFSEAFNTSVR
                     FTDNARAEMDDTWRVWTGIPSDSVGRFPLAKRISLPPAPIRLASRQTRDQTGLHSTLR
                     IDDLTCGPAAGPNRPFAFKADYADPDGVAEIAYAILPGPAVWDNRSADEQQKTAWLPG
                     KNAAVIEPDLTALADGIHHLVVRARDGQGLWSQPTDVPFLFDRKPPEIHHALTDVDHY
                     NGSCLTITLTDPVAPPVLNTLRLTHLGTPINLKQDNGFCTVGQGKIAFELDWIWLLRE
                     QLAAVKHGATLPLSLGGITDAAGNALPPVKIEIPVNLEKDTRPPTILPTAFPTNMLWC
                     EPALTTLDPFFTESQNVQATTGVTPDGRALEITANGERASYIRHTFGPTWDPDIYPWL
                     AISFRTVGEPREGRPFTLAFNTGARRPRGIKEAHTLNLKDTNHLAYVTGALNLQTGVW
                     NDVLIDVRSFLLQETDEHKNTPDITYLSLFFRHKAKGSAVQIRSLSILSPWHSALQIP
                     IRAYDLSTVAGLQWKGGRSQATGIRPANLQLPPDDPLWFRFRLQDGRGNLTDTWMIPV
                     PPGSQNSNPALSGLEPVVF"
     gene            complement(7730..9283)
                     /locus_tag="PK576_05455"
     CDS             complement(7730..9283)
                     /locus_tag="PK576_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE61074.1"
                     /translation="MSYTPRLTKPVPAEYLRDESRKTGVAEAIAFPRDRSELTALLRA
                     ADTAGLPLTPQGARTGIAAGAVPDGGLVINLSRMDRILACCRETPDRTLLRVEPGLPL
                     VLLQRHLSDRRLFPDPLFFPPDPTEPSATLGGMAACNASGARSFAYGATRRHIEAVTA
                     VLADGDTLTLRRGRERAAGRRFAITTESGRRLEGDLPLLPVPEVKNAAGYAARPDMDL
                     IDLLLGSEGTLAILAELELRLSPAPAGILGIVAFFNEEPQALTLVEQIRQQAAAAPSP
                     LLTAIEYFDAEALLLAHEGAAQTGVPLPSLPSRGRNAVYLEWALNDGPDAALDRTARL
                     VAAAGGNAVDTWVAHETVALGRLKTFRHAVPEQINALIAERARAHPGLTKLGTDFSVP
                     DAKLRPLLRRYREDLTAANLQHVIFGHIGNNHLHVNILPRNMDEYVMGKRLYHSWAAQ
                     VVAWGGSISAEHGVGRLKRELLREMVGAEGLKAMRRIKRLFDPAGRLNPGCLFDPNVP
                     SAFSLPPPV"
     gene            complement(9596..11047)
                     /locus_tag="PK576_05460"
     CDS             complement(9596..11047)
                     /locus_tag="PK576_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOE61075.1"
                     /translation="MIKAVVSGVILAGLTVNGAENAASADTFRVGFNRIDITPPLGTP
                     ISGYYNRRIADGVLDPLYARCLALSDGESRALVYSVDNLHITDAVIDEIKAEITRKTS
                     VPAEAIFIACSHTHTGPSTALSGSVNTLPEDEDTIRFSNRILASRCADAGVRALEDLA
                     PAKILLGRGEAKNISFIRRFRMKDGTTRTNPGTNNPDVECALGEPDEQVQLVRFVRDG
                     KKEVALINFQCHPDVVSGTKFSADWPGLACTYLENAMGNEIEALLINGAQGDTNHLRP
                     KTAPGEIVPRRYEMSRHMARTIAGAALATWGMCAPAPSGKVHAAMPKVKVLTNKGKPE
                     ELPLAEKYVRLHKSGRANEIPGIGMERTANTAGAYRILELKDAPDEVDLRVSVVTVGT
                     TLAFGGFPGEPFTWMGTELKAKSPFAMTIPACAVNGSRGYFPVKSAYGPGGYENATSR
                     FKAGTAERLVDGALLKLAELYKAAAPCETKSAK"
     gene            11410..13266
                     /locus_tag="PK576_05465"
     CDS             11410..13266
                     /locus_tag="PK576_05465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE61076.1"
                     /translation="MAGLWLADYGGMYTCAIPETTVGLETQHAVLRFADVQFFEQAPH
                     AVRIQFGVFGWRPNERRWFGLMFCRMRRLVMTWRVSQWMILVLAFLLPGASLLGAGER
                     APSFSLPDLDSGEQVSLDDFRGQIVVLDFFNASCGDCARISWELESEVQAYYEARSGN
                     PHGIAVQVVAVNSETADQEDMDAFLRQTELGMVLDDPKGSLLQRYGGASLPYLVVIDA
                     SVTGPGAAVPRVVLRQAKYGGLKKLRDVIDAITGQAVPTGSGPDTPADTKPETEFSLP
                     ILETGQEVTHETTLDVAAMIASDVYVADALASYRHKRPSTEFTLAASYRPTGMDFKAA
                     PDGLKRDKRLEEDFFAVQGGVRSDLNETLKLSLSGGAYDGFQTYRALWMDEYNRLKFR
                     DVPGYRDADPWGYNMSPSLRWEYLPDAGFAEAGVSYQYDKVSPGYEGGSPLVRLRDDY
                     ETVGGHLSFENVLTRRLRTMVEGRIDDTTKREPRVTLQGGIHYALAEDWVARLAVGYV
                     KENPDFSAKTASVALERDWRGIWFVSVFGRYYKDNGEIDNGIVNEAAAPSIQTYQVGL
                     GVRRKGNRSIIKFDIGPCFSRYDLGANRNRDFDQLYKDRDWLALQAAYQYQF"
     gene            complement(13393..14097)
                     /gene="rsmI"
                     /locus_tag="PK576_05470"
     CDS             complement(13393..14097)
                     /gene="rsmI"
                     /locus_tag="PK576_05470"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOE61077.1"
                     /translation="MLYLVATPIGNLADLSPRALETLKSAQLIACEDTRRTWALLSAF
                     NIPRPELISYRQGNEERAGERVLDAVRAGHTVALCSDGGYPGISDPGYRLIRRAVKED
                     VPFTVIPGASAVDLTLLASGLSTSSYTFKGFPPRKPGALQRFFEEEREQPHTLICFES
                     PFRIAQTLKAAYQVLGDREAAVCIELTKVHERVHRGYLSELIPLFDGKPVKGEVSLAF
                     AGANPKFTRPPDDSTE"
     gene            complement(14052..15194)
                     /gene="hflX"
                     /locus_tag="PK576_05475"
     CDS             complement(14052..15194)
                     /gene="hflX"
                     /locus_tag="PK576_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE61078.1"
                     /translation="MKGYDTHRVERALIAQLVLAGTDPAEAADSLLELGRLAETAGLE
                     VVGQITQNRIKPDGGTLFGSGKVQDLKTLLAETAANTVVFDNPLSAIQAYQLARELDA
                     RIMDRTELILEIFARRARSAEAQLQVELAKTQFLLSRIPVTEAQQRFAGGGGGAYVRG
                     PGESHLSLRNAPLRRRVTELRKRLATIQKRQTVRDRAKREWPVVCLVGYTNAGKSTLL
                     NVLADAGAYVDDRLFATLDTKTRKVWLAPSREILLTDTVGFIRNLPHGLIASFRSTLS
                     VAVEADLLLVVVDAAYPHVTDHINVCFRTLREIGAEQVPHLLVLNKCDRGTADSAILH
                     VTEKYPLALPVSARTGYGLDLLKRAMIEELQRCSTLWRLPSATSRI"
     gene            complement(15191..15706)
                     /gene="lptE"
                     /locus_tag="PK576_05480"
     CDS             complement(15191..15706)
                     /gene="lptE"
                     /locus_tag="PK576_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOE61079.1"
                     /translation="MRLTVSLTAATLLLAAGCASYTCTSAVPEELRTLAVPVFENESG
                     FPEIDAVVTQQVLREFQREGTFRIARQDAASLKLLGKLVKTDMQSLNYDRNYGSRTSE
                     YRYGLVAEITLVERGTGKLLIDAQRVKANTTFLTHGDMLTGVQDAYARAARELARAIV
                     DTVLAHWPSPQ"
     gene            complement(15723..16754)
                     /gene="bamD"
                     /locus_tag="PK576_05485"
     CDS             complement(15723..16754)
                     /gene="bamD"
                     /locus_tag="PK576_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOE61080.1"
                     /translation="MMRQGFIRIIAAAALAAAWCSSFAQQTGRSGFHRGYATDSFEGF
                     EALDKDDRIPQKKKSFWYGVREETAAAQLDYALRQEAAGRDRTARKAYEALIREWPTA
                     PQAAEAQLALAALFERTGKFERAFDEYQYLLTYFSGHCPYDEVLDRQFRIANHLLHNN
                     RSMFGWMLSGTDSIRERFEKIVRNAPRSAIAPEVMLIIGGIRVSERELPEAIAVYDGI
                     LNRFPASPQAMHAAYLAAQCRYDLAMKHTYNEPRCREAIAFSKALLARLPNHPQKEQL
                     TVWLNALTEQLIDQNYQRAVFYDTRQRNATAAQAAYRRFLSEFADSKYAEQVRARLVE
                     LDRDAAPAP"
     gene            16938..>17538
                     /locus_tag="PK576_05490"
     CDS             16938..>17538
                     /locus_tag="PK576_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE61081.1"
                     /translation="MKKVLIPTKLDVVAAELLKAHGGYAVVQDAKTPLEELVKEHADA
                     YALIVRSEKVTAAIIDALPQLKVVVRAGAGYDTIDIKHARKKGVDVMNTPGANANAVA
                     EEVIAMMLADARHIVQADTTTRAGGWEKKNYMGREISGKTVGIVGLGNIGRLVAKRLR
                     GFECRILGYDPLVNRDRLRDLGIESATLEEIFSQSDYVTL"
BASE COUNT         3112 a         4974 c         6041 g         3411 t
ORIGIN      
        1 gcaggtcgcc cgtcgcgggg cgacataagg tctgacggga gaagcgggcg tccctaccgt
       61 tggagcaggg aatacagttg ggcgacgggc tcaatccttc cgcgccgcat tcgcgtcgtt
      121 tccggaacgg tacgcgtcga gtgcttgccg gaacgcttcg gtctgcgcgt acccgcaact
      181 cttgaactcg gggcagaagc cgcggtagac gcattccggc acacagacgc ggtagagttc
      241 gggttcgtgt tcgcgcatcg cgtccagcac cagacgccag gcctcggtgg tttcggggga
      301 ggcttgctta cagagccgtt tgcgtgaaat gaagatcagg gcctgggcgt tcgcgaagca
      361 ctcgtggtca acaaggcttg cctgcggcat ggtgtcgcgg ttgacgccgg tacggtccga
      421 gcgttgggtg ctcacgaagt gttcgattcc gattttgtgc cgcacgaagt gcacgctgac
      481 ccagtatttc agattgaccc atttccattt gaagagcatc tggcggatgg gtgagtgttc
      541 cgaaagcagg atgcggtgtt tccaggcgtt gctgggttcg cggtcgccct cctccatgcg
      601 gatggtggtg cgggcggcgt cggcgacatc gcgccatacg gacttggttt tgaagtattt
      661 caagatgggg ggctgactca tgcgacgttc ctttcagtat gcgggtgaac gaaaacgggt
      721 taggggctcg ggcacaggcc accatccacg cgcaggatct ggcccgtgat ccaagcggcc
      781 tcttcggatg cgagaaaccg cacgactccc gcaacttcgt cgggtgtgcc gaaacggcct
      841 agcgggatgg cggcgagttg tttgacgcgc cgggtctcgg ggatctcggc ggtcatgtcc
      901 gtggtgatga tgccgggggc cacgacattc accgtgactc cggaggcggc cagttcgcgg
      961 gctgcggtct tggcgagtcc gaacagggcg gccttggagg aactgtaggc cgcgcgcatg
     1021 agatcgccca tcaggcctgc ggccgaagcg atgtagatga tccggccggc ctgttggcgc
     1081 gccatggggc gcgtgagggc tttggttagc aggaacgcgg cgtcgaggtt cgcggcgtga
     1141 acctctcgcc aggtgtcgag cccggtgaag gcgaggagct ggcttcgcag gatgccggcg
     1201 ttgctgacca gggtgtggat cccgccgcag gttccgatga cccgcttgac cagttcatcc
     1261 acttctgcgg ggacagtcag gtctgcggcg aagggttcgg ctttgccgcc ggctttgcgg
     1321 atcgccgcga cagtctcttc cgccgcctca accgaggtgt ggtagtggag ggcgatccgg
     1381 aaaccgtcgt gcgcgagacc gagcgcgatc gcgcgtccga tcccttttgc agctccggtg
     1441 atgagggcgg ttttcatgcg ggaagatatg gcgcgaggac ggtttcgtca atcagatccg
     1501 cgccggtttc ggcccggacc ttttccagat cgatgtgatg ttcgcgggct aagcgccggg
     1561 cgcgcggggt cgcgcggata cgcgtgccgc ggtcggttcc cgaaccggcg gaagcggtgc
     1621 cgcggtacgc tgcggtgagc gcggcattct tttccggaat cgaggggtcc tcctcgccgg
     1681 gggcaccgat gaggcctgcg atgaaaccgg tcggcacgac gctcttcgcg gtggcataga
     1741 ccgcgagcag tgtgccggag gcgggggctt ccagttcgta cgctgccttg tccgtggcta
     1801 gctcggccac acattcgccg gcctcgaccg tgtcgccgac ttgtttgcgc cagcgcgaga
     1861 gggtcacctc gaccacattc gcatcaagtt ggggaattag aatttgtgtc ataacggttt
     1921 tgaggttggg ggttaacggt taggttggat cagaagacaa cgggttccag tcctgacagg
     1981 gctggattgc tgttctgcga gccgggcggc acggggatca tccacgtgtc ggtcaggttc
     2041 ccgcggccgt cttgcaatcg gaagcggaac cagaggggat cgtcgggcgg gagttgcaag
     2101 ttcgcgggcc ggataccggt ggcctgcgag cgtccgcctt tccattggag gcccgcgacg
     2161 gtgctcaggt cgtacgcccg gatcggaatc tgcaacgcgg agtgccaagg cgagaggatg
     2221 gagagggatc ggatttggac ggcgctccct tttgccttgt gacggaaaaa caacgagagg
     2281 tacgtgatgt ccggcgtgtt cttgtgctca tcggtctctt ggagcagaaa cgagcggaca
     2341 tcgatcagca cgtcattcca cacgccggtt tgcaggttga gcgcgccggt gacatacgcc
     2401 agatggttgg tgtccttcag attgagcgtg tgcgcttcct tgatgccgcg gggccgccgt
     2461 gcgccggtgt tgaacgcgag ggtgaagggg cggccctccc gcggttcgcc gaccgtgcgg
     2521 aacgaaatcg cgagccaggg gtagatatcg ggatcccaag tcggcccgaa ggtgtggcgg
     2581 atgtacgagg cgcgctcgcc gttcgccgtg atttcgagtg cccgtccgtc cggtgtgacg
     2641 ccggtggtcg cctgcacgtt ttgcgattcg gtgaaaaagg ggtcgagcgt tgtcagggcg
     2701 ggttcgcacc agagcatgtt tgtcggaaaa gcggtcggca ggatggtggg cgggcgtgtg
     2761 tctttttcga ggttgaccgg gatctcgatt ttgacggggg gcaaggcgtt gccggccgca
     2821 tcggtgatgc cgccgagcga gagcggcaac gtcgcgccgt gcttgactgc ggcgagttgt
     2881 tcgcgcagca gccagatcca gtccagctcg aatgcaatct tgccttggcc cacagtgcag
     2941 aacccgttgt cttgcttgag gttgatcggg gtgccgaggt gggtgagccg cagagtgttg
     3001 agcactggcg gtgccaccgg gtcggtgagg gtgatcgtca ggcagctgcc gttatagtgg
     3061 tccacgtcgg tcagggcgtg gtggatttcg ggaggtttgc ggtcgaacag aaaggggaca
     3121 tccgtaggtt gggaccaaag gccttggccg tcgcgcgccc gcaccacaag gtggtggatg
     3181 ccgtcagcca gcgcggtgag gtcgggttca atcactgcgg cgttcttgcc cggtagccaa
     3241 gcggtctttt gctgctcgtc ggccgaccgg ttgtcccaga cggcaggccc cggaaggatg
     3301 gcgtaggcga tctccgcgac accatccgga tcggcgtagt ccgctttgaa ggcaaagggg
     3361 cggttcggtc ctgcggcggg tccgcaggtc aggtcatcga tccgcagtgt gctgtgcaga
     3421 ccggtctggt ctctcgtctg ccgcgaggcc aggcggatcg gcgcgggcgg caggctgatg
     3481 cgtttcgcga gggggaagcg gcccacgctg tcggacggga tgccggtcca gacacgccag
     3541 gtgtcgtcca tttccgcgcg cgcgttatcg gtgaaccgca cggaggtgtt gaacgcctct
     3601 gagaacgtga acgcgaactc gccaaaggcg agcgaaacga tcgccatggg gtcgcccttg
     3661 taccggaact ggagcagcgg cgtggcggcg atgtcgaagc gcgggaagag ttgtccggtg
     3721 aggcgccgtc cgtatacgcc cgcgttgctg aaggcgagca caccgccgcg acggggatcg
     3781 gtgacctcca ccgcgacgcg ggccgccatc ttatgggggg tcgtgtcgaa tgggcgggcc
     3841 tcaaaggttt caaccgcgcc ttccggcatg gtgagcgaaa ggaggaccgg cggggagttc
     3901 acgctgtcct ctttccgcat ggggacgacc tgacggaaga aacgtccgtc atacagttcc
     3961 agcgcaaggg tcatgcggcc cggctgcaaa atattcagcg gcacgagaat ccggatcgtg
     4021 ttgccttccg tataaaatgg cgcgggctgc gcatcgactt gcacttgctt gggcgggagc
     4081 atcggcgagg gccaggggtg ggtgggggtt accacaatcg agccgaggac agcggggtcg
     4141 gactgtgcga gcaggccgaa tttcgcccgt tccggcacgg cccattcgat cacaggtcgg
     4201 cgcgaatcga gggctgcagg cagttcgacc atttgcagtt cgccggggct gaggccgttt
     4261 atgagttgag tgagggaggc gatctcggcc cgtttttcgg gcgggccgct cacggtcgga
     4321 atcccgcggt ggatctcacg gaaatgacgg atcgcatacc aggcgtgggg cgggttgcct
     4381 tccagccctt gttgcacgtc gctgggctgg aggttgccga agccttcgat ctgcacggcc
     4441 tgattggcgc ggatgatttc ggagggcacc cagatctcga ccggggtcca gacccggttt
     4501 gtgcctgcgg gatcggatgc gggcagggat tcaagggcac cgcagatttt gcgcgggccg
     4561 cggctttcgt tcgagccgca gagaacgtag gtccagtgct ggatcggcgt ccagccgcct
     4621 ttgccgtcct ccttgacggt ggcgaactca aagttgacgc gggcctgcgg atgtcgcgca
     4681 atctcgaagc gccatcccag cagcttgtcg ggttgcgcgg gcggcagcat gcagcgcacc
     4741 aggaaggagc ctgcgccgag cagggaggtc acatacattt cgggacgggc ggtttggaga
     4801 tggcggaaac gcacgttggg gtggctcacg gtgtcgtaag cctgccagaa atccggagtg
     4861 agcggttggg cgacacggcc attgatacgg atgccggtgt cggcgggagc tgctgcaggt
     4921 tcggtggggg gcggtgccgc gggaggaagc gcccggaagg cgaagggccg gggccggatg
     4981 gactccgcgg cgatcttgat gcgtgcgacc atcatcgaat tccggtaggt ccagatgcca
     5041 agcgcccctt cctgaagcgg atcgggatcc tcgatgcgga agacatcctc gttatcatag
     5101 atgtagtgca gggttttgcc cacgcgccgc aactgcatgc cgtaccaggc accgtggatg
     5161 tcgcggcccc cggccaccag cggctggtag ccttggcggg cgaggcccgt gcgggcgcgc
     5221 ggcacaaggt acttggtgga gaggtcgaga aggcttccgt tccggatcag gcgggagtag
     5281 agttgcgaat ggtccggatc ccatccggtg gcaatcgcgg tgtacccgtc gccggttgag
     5341 agagaacggc tcatcatggt gaggttaagg tcgcctggcc gcgcatacca gcccatgcgc
     5401 atccccgcgt aaaattcgat gctgaaatcg ccgcggaaga catatttgga ccagagcgca
     5461 gcgagcgagt cagcgctttc cccgttcata tgcgaccaag taggctcgca atagaaccgg
     5521 ttgatgacct cccaccggcc gccgttgatc gtccagttgt agagggattc cgtgaagagc
     5581 gtgtcaaaca cgttgtcgcg tttcaccagc gtccggctca gattgtccag cgtcatgccg
     5641 ctgatccgca tgcggcggcc atgcaacggc gcgtgcagat gggtttgggc aagcagaagg
     5701 gtgtcgccgc cgagccagag gacggagcct tccagtccga gggtgaaaag acgcagcttg
     5761 gtgttgttga actcgacttc gggcacctgg tcggccgaca cctcgaaggc cgtattcgtt
     5821 ccgctttccg gtgtgaagac gcggatgacc ccttttttca cagaaacgcg gcacaggccg
     5881 ttggagagcc cctccggaac gccgagatgc agttccattt cctccacgac cggcagccgg
     5941 acccgcaccg cgccggtgat gtcgcctttg aaccacgtca tgccgttttt gaaagtaatc
     6001 cattgacctt ccggagaggc ccagtgccgc atgaacggat cattgacata caaggggttt
     6061 ttctcgaagc gctcggagag tcccgtctca cgcgaggtga cggtcggcgc ggtgaaaaaa
     6121 agatcgccgg gggttacggc cagaacgccg agttcaccgc cgggctgatc ttccgggcga
     6181 tggaagcaga ccagaaggtc gtcggcatag gcacgaagtt cgtgcggccg cagggcgtcc
     6241 agagcgagga tcacccggtt gctggagacc ggcatttgga aggagtccag aagaacggtg
     6301 ccgttggttg tcacatgttc gagccggaag gtgcggccgc ccccatcctg ttcacaggtg
     6361 aaacggtatg gggccgcacc gtctccggaa ccgcccgcgg tcagaccgca caggaaggag
     6421 tcggtggtgg caaccacccg gatctcctgc cgcaacgggc cgtcgtcgga actgccgtac
     6481 gcccacattt gcgtggttgt ggcggagggc ggaaaataga gccatgccgt gtcgtgacgc
     6541 tgcagggagc gcacgtccgg gctttggcgg cgcgtggcaa aggtgacatc ctcgggcgag
     6601 tcgaaaaggc gatcctgatg cgatgccgcg gtcacatcat cgaaacgggt ggtttcccct
     6661 gcgggcatgt ttgcgaaaag cccgaaacgt ccgcccggcg gcaggccgat cttatggcgg
     6721 atcacctcga tgttgtcggc gcggcaaagg atacggtcgt caaacgtctg cacctcaagc
     6781 aggagccatt ggcctgcggg cagttcggtt tgcacgcccg cgagatacag gcggggtgcg
     6841 ttgggcgcgg ccggctgctg ccacaagtcg aggatcagac ggtccgacac cggatccgtg
     6901 cgggccgtga aggcaaagta gccgccgtgc tcgcccgcca agaaaaccag cccgttggtg
     6961 ccggtgttgt gctggactgc ggcacggaag acataacggc tgtagaaagg ttcgcccgcc
     7021 aggacgatgg cgttggtccc gccgccctcc agcgtgtaga agttcgggct cttttccggt
     7081 ttcggttggc gcgccagttg atagccgccg gacgagccgc tgatcgaacc ggtgaccgaa
     7141 tgcaggcgcc agatgccgct ttggatatcc caagtggggg ggaaggccga accggccgga
     7201 accaaaaagt tgtcctcaaa cgtgaaggag ccgaggcgtt gcgtgtactc atcggcctcc
     7261 gcctctggcg gctggagcgc cggggggtgt tccacgttca ccgctccctg ccagagttcc
     7321 ggcagacgcg ccaccggccg gtcgtccacc gtgaggatcc agacgtccgc atggcgtttc
     7381 agggtgatcc agtggagcgg accggcgagc gacgggcacg gataagtggt gacgcggcgt
     7441 tcggtcgcgt catcgaagaa cccgcggggc aacaggcgga agcgggtatc cccttcgctt
     7501 aacgtgaact ccgcctggca ggtgtgatcc ggatggctga ggcggagacg caacgcgccg
     7561 cgttccggcg tcagattcag ggcaccccat tccgaccgtt cggtcagatc cagacgggtg
     7621 gaccacgcct cttcagccgc caaccctgcg gtggcggcgc aacaccccca tgtcagcccc
     7681 gcccatatca aaacgttcgt ccagttcacg cgatttagtt taacaggttt tacaccggag
     7741 gggggaggga aaatgcggac ggaacgttcg gatcgaagag gcagccgggg ttgagccgtc
     7801 ccgcggggtc gaacaaccgc ttgatccgcc gcatcgcctt caggccttcg gctccgacca
     7861 tctcgcgcag cagttcgcgc ttgagacggc cgaccccgtg ttcggcggag atgctgccgc
     7921 cccaggcgac gacctgcgca gcccatgagt ggtagagacg tttccccatt acgtattcgt
     7981 ccatattgcg gggcagaatg ttgacgtgga gatggttgtt gccgatatga ccgaaaatga
     8041 catgttgcag attggctgcc gtgagatctt cgcggtaacg acgcagcagc ggacgcaact
     8101 tggcgtccgg cacggagaaa tcggttccga gtttggtgag gccgggatgc gcgcgcgcgc
     8161 gttccgcaat caaggcgttg atctgttccg ggacggcatg gcggaaggtt ttgagtcggc
     8221 ccagcgcaac cgtttcgtgc gcgacccaag tgtcgacggc gttgccgccc gcagcggcga
     8281 ccagacgggc ggttcggtcc agagcagcat ccggtccgtc attcaaagcc cactcgagat
     8341 agaccgcgtt tcgtccgcgg gacggcaggg acggcagggg tacgccggtc tgcgccgcac
     8401 cttcgtgcgc cagaagcaga gcctccgcat cgaaatactc gatggcggtg aggagaggcg
     8461 agggggccgc cgcagcctgc tgccggattt gttccaccag cgtcagggcc tgcggctcct
     8521 cattaaaaaa cgcgacgatg ccgaggatgc ccgcaggcgc gggtgagaga cgcagttcga
     8581 gttccgcgag aatggcgagc gtcccctcgc tgccgagcag caggtcgatc agatccatgt
     8641 cgggacgggc cgcgtatccg gccgcatttt tgacctccgg cacaggtaga agaggcagat
     8701 ccccctccag acgacgcccg ctctccgtgg tgatcgcgaa tcggcgccct gcggcccgtt
     8761 cgcggccgcg gcgcagcgtc agggtgtcgc cgtccgccag gacggccgtg accgcctcga
     8821 tgtggcgccg cgtcgcgccg taggcgaagg agcgggcgcc ggaggcgttg caagccgcca
     8881 tgccgccgag agtggccgac ggctcggtgg ggtcgggcgg aaagaagagc gggtcgggaa
     8941 agagccgtcg atcggaaaga tggcgttgga ggagcacgag cggcagcccc ggttcgacgc
     9001 gcaggagggt ccggtccggc gtctcccggc aacaggccag gatccggtcc atcctcgaaa
     9061 gattgatcac caggccgcca tcaggcacgg caccggccgc aatcccggtg cgcgccccct
     9121 gcggggtgag cggcaggcct gccgtatccg ccgcgcgcag gagggcggtc agttcggagc
     9181 ggtcgcgcgg gaaggcgatc gcttcggcca cgccggtctt gcgcgattcg tcccggaggt
     9241 attcggcggg gaccggcttc gtcaaccgtg gcgtataaga catgacgccg atcataccgg
     9301 gacgcctccc gaagccgcaa gcgcaaagcg cgcgccttta cgggcaagca gacggtccct
     9361 gccattttga tttcatgaag tttcaaggtt tcatccaaat cgctatcggg atcgctatcg
     9421 ggatcgggat cgctatcggg atcgggatcg attgtttaac cacgggacac acagaacaca
     9481 cggaaaggca tggctctgtt ttcgtttgtt tgacgccgtt cgtggtttca atcagaagtt
     9541 gcgttttgca ccaaatcggt gcagcgcgga tctcaccgaa ttttagggtt aatccttatt
     9601 tcgcgctctt tgtctcacat ggggcggcgg ccttatagag ttcggccagc ttgagcaggg
     9661 ctccgtccac gagacgttcg gcggtgcccg ccttgaagcg gctggtggcg ttttcgtagc
     9721 ctccggggcc gtaggcggat ttcaccggga aatagccgcg cgaaccgttg acggcgcaag
     9781 cggggattgt catcgcgaat ggcgactttg ctttaagctc cgtgcccatc catgtgaagg
     9841 gttctcccgg gaaaccgcca aaggcgagcg tcgtgccgac cgtcaccacg gaaacccgca
     9901 ggtcgacttc atcgggggcg tccttgagtt cgaggatgcg gtaagctccg gcggtattgg
     9961 ctgtgcgttc catgccgatg ccgggaatct cgttggcgcg tcccgacttg tgcaaacgga
    10021 cgtatttctc ggcgaggggc aactcttccg gtttgccctt gttcgtgagg actttcactt
    10081 tgggcattgc cgcatgcacc ttgccggatg gcgcgggcgc gcacatcccc cacgttgcaa
    10141 gcgcggcgcc ggcgatcgtg cgggccatgt gccttgacat ctcgtagcgt ctcggaacga
    10201 tttctccggg agctgttttc ggacggaggt ggttggtgtc gccctgtgcg ccgttgatca
    10261 gcagcgcctc gatttcgttg cccatcgcgt tttcgagata cgtgcaggca agccccggcc
    10321 agtcggcgga gaactttgtg ccgctgacga cgtccggatg gcattgaaaa ttgatgagcg
    10381 cgacctcttt cttcccatcg cggacaaaac ggacgagctg tacctgttcg tcgggttccc
    10441 cgagcgcgca ttcaacatca gggttgttcg tgccgggatt ggtgcgcgtg gtgccgtcct
    10501 tcatgcggaa gcggcggata aaggagatgt ttttcgcctc gccgcgaccg agcaggattt
    10561 tggcgggcgc gaggtcttcg agcgcccgga cgccggcatc cgcgcaccgc gaggcgagga
    10621 tgcggtttga gaagcggatc gtgtcctcgt cttcgggaag cgtgttgacg ctgccggaca
    10681 gcgcggtcga gggtcccgta tgcgtgtgcg aacaggcgat gaaaatcgcc tcggcgggaa
    10741 cgctcgtctt gcgggtgatc tccgccttga tttcgtcgat caccgcatcc gtgatgtgga
    10801 ggttgtcaac ggaatacaca agcgcccgcg attcgccgtc cgacagcgcg aggcaacgcg
    10861 cgtagagcgg atccagaacg ccatccgcaa tacgacggtt gtaatagcct gagatgggtg
    10921 tcccgagagg gggggtgata tcgattcggt tgaagccgac cctgaacgtg tccgcggaag
    10981 cagcgttttc cgccccgttg accgtcaggc ctgcaagaat aacaccggac acgactgcct
    11041 taatcatgcg tttctccctt acgtttaacg gtaaccgatg cggggacacc ccgcgatgta
    11101 caagtcaatc tctcaaaaac gcgccagggt ctcaagctgg aattcattat gaatactagg
    11161 ccaaccgtgt tcaacgtgcg agaacgcagt cgggtttggg gatgtttctt gatgttcggt
    11221 attgacgccg aatagcgaaa gaattatcat aacatttctt ttaagcatga ggcggttccg
    11281 gtcaggccaa gcgggcgggc ggggtccggg gtccggtatg ttgtggagct ttctgatgac
    11341 cgatgtatct acctttaaga tcaggacgcg gggcggtatc ccgtgccgag gtcgttgcgg
    11401 tttgttggca tggctggttt gtggctcgct gattatggcg ggatgtatac atgtgccatc
    11461 ccagaaacaa cggttggtct cgaaacccaa catgcagttc tacgattcgc cgatgttcag
    11521 ttctttgaac aggctcctca cgcagttcga atccagttcg gcgtcttcgg ttggcggcca
    11581 aacgagcggc ggtggttcgg gctcatgttc tgcaggatgc ggagattagt gatgacgtgg
    11641 cgtgtttctc agtggatgat tctggttttg gcgtttttgc tgccgggagc gagtttgttg
    11701 ggcgcagggg agagggcccc gtctttcagc cttccggatt tagattccgg agaacaggtt
    11761 agcctcgatg attttcgggg gcaaattgtt gtgctcgatt ttttcaacgc gagttgcggg
    11821 gactgtgccc gcatttcgtg ggagcttgag tccgaggtcc aggcgtacta cgaagcccgt
    11881 tccggcaatc cgcacggtat tgccgtgcag gtcgtcgcgg tgaacagtga gactgccgat
    11941 caggaggaca tggatgcctt tttgcggcaa accgaactgg gtatggtgct ggatgatcct
    12001 aaaggttcgt tgctgcaacg ttatggcggc gccagcttgc cctatcttgt ggtcattgac
    12061 gcctcggtca cgggacccgg tgccgccgtg ccccgggttg tgctgcgtca ggcaaaatac
    12121 ggaggcttga aaaaactgcg cgatgtcatc gacgcgatca ccggacaggc cgtgccgacg
    12181 ggatcaggtc ccgatacacc ggctgacacc aagccggaaa ccgagttctc cctgccgatt
    12241 ctcgaaacgg ggcaggaggt tactcacgaa accacgctgg atgtcgcggc catgattgcc
    12301 tcggatgttt atgtggcgga tgcgctggcg agctatcgtc ataagcgccc atccacagaa
    12361 ttcactcttg cggcttcgta tcgtccaacc ggaatggatt tcaaagctgc ccccgacggg
    12421 ctcaagcgag acaaacgact ggaagaagac ttcttcgcgg ttcaaggcgg tgtccgttcc
    12481 gacctgaacg aaaccctcaa gttgagtttg agcggtggtg cctacgatgg gtttcagaca
    12541 tatcgcgcgt tatggatgga cgaatacaac cggctcaagt tcagagacgt tccgggatat
    12601 cgggatgccg atccgtgggg atacaacatg tctccaagtc ttcgttggga ataccttccc
    12661 gacgctggat tcgcggaagc cggtgtctcc taccagtacg acaaagtttc gccgggttat
    12721 gagggggggt cacctcttgt ccggctgcgc gatgactatg aaacagttgg cggccatctg
    12781 tcgtttgaaa atgtgctgac acgccgcctt cgcacgatgg tggaagggcg gatcgacgac
    12841 accacaaaac gggagccgcg tgtgaccctg caggggggga ttcattacgc gttggcggag
    12901 gattgggtcg cgcggctggc tgtcggctac gtaaaggaaa accccgactt ttcagcgaaa
    12961 acggccagtg tcgcgctcga gcgggactgg cgtggcatct ggttcgtaag tgtgttcggg
    13021 cgttactaca aggacaatgg cgaaatcgac aacggcattg tcaacgaggc tgccgcgccg
    13081 tcgattcaaa cctatcaggt cgggttgggt gtgcgccgca aggggaaccg ctccatcatt
    13141 aagttcgaca tcggcccttg cttcagccgg tatgaccttg gcgccaatcg gaatcgcgat
    13201 ttcgaccaac tctataaaga ccgggactgg cttgcattgc aggccgcata ccagtatcag
    13261 ttttgagggg tggcctctgg cgtggcggac tgcgtccacg cgtggaaaac gcgccaggtg
    13321 cgcgtctttg tccaacgggt gtcgtgcgat agttgcggcg gggaacgttc cggatgggcg
    13381 gttcgtgcgg gatcactccg tggaatcgtc cggtggccgg gtgaacttgg ggttcgcccc
    13441 ggcgaacgcc agcgaaacct cgcctttgac cggtttgccg tcaaaaagcg gaatcagttc
    13501 cgagagatag ccgcggtgta cgcgctcgtg taccttggtt aactcaatgc agacggcggc
    13561 ctcgcggtcg ccgagcacct gatacgcggc tttgagcgtc tgcgcgatcc ggaagggcga
    13621 ttcgaagcag atcaacgtgt ggggctgctc ccgctcctct tcaaaaaacc gttgcagggc
    13681 accgggtttg cgtggcggaa accctttgaa ggtgtaactc gatgtggaaa ggcctgaggc
    13741 gagcagagtc agatccacgg ccgaggcgcc ggggatcacg gtgaacggga cgtcctcttt
    13801 cacggctctg cggatcaggc ggtagccggg gtcgctgatg ccgggatagc cgccgtcgga
    13861 acacagtgcc acggtgtgcc cggcacggac tgcgtcgagg acccgttccc cggcacgctc
    13921 ctcgttgccc tggcggtagg agatcagctc gggacgcgga atgttgaaag cgcttaggag
    13981 tgcccaggta cgccgcgtgt cttcgcaggc gatgagctgt gcgcttttca gggtctccaa
    14041 ggcccgcggg ctcagatccg cgaggttgcc gatgggagtc gccacaaggt agagcatcgc
    14101 tgaagttcct cgatcatggc ccgcttgagc aggtcgagcc cgtagccggt ccgggccgag
    14161 accggcagtg cgagcgggta tttctcggtc acatgcagaa tcgcgctgtc ggcggtgccc
    14221 cggtcgcact tgttcaggac cagaaggtgc ggcacctgtt ccgcgccgat ttcgcgcaac
    14281 gtgcggaaac agacgttgat gtggtcggtt acatgcggat aggcggcgtc gaccacgacc
    14341 agcagcagat cggcctcgac cgcgacactg agggtggagc ggaaggaggc gatcagccca
    14401 tgcggcaggt tacggatgaa gccgacggtg tcggtcagca gaatttcgcg gctgggggcg
    14461 agccagactt tgcgcgtctt ggtgtcgagc gtggcgaaaa ggcggtcgtc cacataagcg
    14521 cccgcgtccg cgagcacgtt gaggagcgtt gacttgccgg cgttggtgta gcccaccaga
    14581 cagacaacag gccactcgcg cttggcgcgg tcacgcacgg tctgacgttt ctggatcgtt
    14641 gccagacgtt tccgtaactc ggtgacacgc cgccgcagcg gcgcgttgcg caggctgagg
    14701 tggctttcgc cgggaccgcg tacgtaggca ccgccgccac cgccagcgaa gcgctgctgc
    14761 gcttcggtga ccgggatacg cgagagcagg aactgggtct tggccagttc gacttgaagt
    14821 tgcgcttcgg cggaacgcgc gcggcgcgcg aaaatttcga ggatcagttc cgtgcggtcc
    14881 ataatgcgcg cgtcgagttc gcgcgcgagt tggtacgcct ggatcgcgct gagcgggttg
    14941 tcgaacacca ccgtgttcgc ggcggtctcc gccagcaggg tcttcagatc ctgcactttg
    15001 ccggagccga acagggtgcc gccatctggt ttgatccggt tttgggtgat ctgtcctacg
    15061 acctccagac cggcggtctc ggccaggcgc cccaactcca gtagcgaatc ggcggcctcg
    15121 gccggatcgg tgccggccaa cacgagttgg gcaatcaggg cacgttcgac acggtgtgtg
    15181 tcgtacccct tcattgcgga gagggccagt gggcgagcac ggtgtcgaca atcgcacgcg
    15241 ccagttcgcg tgccgcgcgc gcgtaggcgt cctgcacgcc ggtcagcatg tcgccatggg
    15301 tcaggaaggt ggtgttggcc ttgacgcgct gggcgtcgat cagcagtttt ccggtgccgc
    15361 gttcgaccag cgtgatttcc gccaccagac cgtaacggta ttcggaggtg cgggaaccgt
    15421 aattgcggtc atagttcagc gactgcatat cggttttgac cagcttgccg aggagtttca
    15481 gcgaggccgc gtcctggcgg gcgatgcgga acgtgccctc gcgctggaac tcgcgcagca
    15541 cctgctgcgt gaccacggca tcgatttcgg ggaaccccga ctcgttctca aaaaccggaa
    15601 cggccagggt gcgcagttct tccggcacgg cggaagtgca ggtgtaggag gcgcagccgg
    15661 cggccagcag cagtgtcgcg gcggtcaagg aaacggtcag acgcatggga ctctcctttc
    15721 attcatgggg caggggcggc atcccggtcg agttcgacga ggcgtgcgcg gacctgctcg
    15781 gcgtatttgg aatcggcgaa ttcggacaaa aagcgccggt aggcggcttg cgcggccgtg
    15841 gcattacgct ggcgggtgtc gtaaaagacg gcgcgctggt aattctggtc gatgagctgt
    15901 tcggtcaggg cgttcagcca gaccgtcaac tgctctttct gcgggtggtt cggcaggcgg
    15961 gcgagcaggg ccttggagaa ggcgatggct tcgcggcagc gcggttcgtt gtaggtgtgt
    16021 ttcatcgcca agtcgtaccg gcactgcgcc gcgaggtagg cggcgtgcat cgcctgcggg
    16081 gaggcgggga agcggttcag gatcccgtca tatacggcga tggcctcggg aagttcccgc
    16141 tccgagaccc ggatcccgcc gatgatcaac attacttccg gagcgatggc cgaacgcggc
    16201 gcgttccgaa cgatcttctc gaagcgttcc cggatgctgt ccgtgccgct gagcatccag
    16261 ccgaacatgc tccggttgtt gtggagcaga tggttggcga tgcggaactg gcggtccagc
    16321 acttcgtcgt acgggcagtg cccggagaag taggtgagca ggtactggta ttcgtcgaag
    16381 gcccgctcga acttcccggt gcgctcgaac agggcggcga gggccagttg ggcttctgcg
    16441 gcctgggggg ccgtgggcca ctcgcggatc agcgcctcat aggctttacg cgcggtgcgg
    16501 tcccggccag ccgcctcttg ccgcaatgcg taatcgagtt gcgcggcggc tgtttcctcg
    16561 cggaccccgt accagaacga cttttttttc tgggggatgc ggtcgtcttt atccaaagcc
    16621 tcaaatccct cgaacgaatc ggtcgcgtat ccccggtgga agccgctgcg tccggtctgc
    16681 tgcgcgaaag aagaacacca ggctgccgcc agggcggcgg cggcgattat ccgtatgaac
    16741 ccctgcctca tcatctgttt ttccgatctg cgagtttcgg tctgccgcgt tgccgtccgg
    16801 cgtaacgggg cttgattccc gttccgcaca agattacagt attaccctgt ttcggacggt
    16861 cggcgaatgg gtcgcggccg accgggactg ccaagccatg tacgttaacg tagcaaaccc
    16921 cggggaactg tagaagcatg aaaaaagttt tgatacccac caagttagat gtcgtggccg
    16981 ccgagcttct gaaagcgcat ggcggttatg cggtcgtgca ggatgccaaa acgccgctgg
    17041 aggagctggt caaggagcac gcggacgcct acgcgctgat cgtgcgcagc gagaaagtga
    17101 ccgccgcgat catcgacgcg ctgccgcagc tcaaggtggt tgtgcgcgcc ggcgcgggat
    17161 atgacacgat cgacatcaag cacgcccgca aaaaaggcgt ggacgtgatg aacacgccgg
    17221 gggcgaacgc caacgccgtg gcggaggagg tcatcgccat gatgctggcc gatgcgcgcc
    17281 acatcgtgca ggccgatacc acgacgcgcg ccggcggctg ggagaagaaa aactacatgg
    17341 gccgcgagat ctccggcaag accgtgggca tcgtcggtct cggcaacatc ggccgtctgg
    17401 tcgccaaacg cctccggggc ttcgagtgcc gcatcctcgg ttacgatccg ctggtcaacc
    17461 gcgaccgcct gcgcgacctc gggatcgagt ccgccacgct ggaggagatt ttcagccaga
    17521 gcgattacgt cacgctcc
//
LOCUS       DAOSLE010000056        16967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3574_length_16967_cov_6.207604, whole genome shotgun sequence.
ACCESSION   DAOSLE010000056 DAOSLE010000000
VERSION     DAOSLE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16967
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_16967_cov_6.207604"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..2362)
                     /locus_tag="PK576_05495"
     CDS             complement(314..2362)
                     /locus_tag="PK576_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61082.1"
                     /translation="MSIGSWVHKNNLAKGMVVCGLLQIAGYYVAGSLATDGFHLPAPQ
                     PDTLLYCQSARQVAEGHPFVYTPGDRPSTGSTSHLYPFALGLLYKLGAKGDALLTAGF
                     ALNALLYLLFLVSWAGIIVRQIRSPRNRFAACALVALSGQAAYSALSQSDAGLFMALS
                     SGLLFTLLSGRMAGFAVLLGLSPWCRPEGVLLSVLFAAALAFKRWGMRQRVTRQEGVA
                     AVVGLLSSAGLFAFNYGLTGHAQFLSVIHKGYAKQYDFFSALHLTLGDMVRILRELLL
                     GLPDALPRESFYVPLIGALCAWIGLVTLPRRREGLWKEVWWLSACAAGVGVVASSGWQ
                     NTNVDRYLAWLMPVWLVYTAAGAMRVGRGWGRTAVCRWGLLLIVAAFQMLNAAWLVTL
                     YHTACLITHPCYEFAKEAHARLPPGARVGGAVCNLAYGMPGRSYAHLSGLYSPDWMTP
                     EVVTNLERLKHHPERRFDYWALPSAQTEFGGSDIAVLCGPVAALGVDENQMRQAVWSP
                     LDGARAPHLADKEVRGWRLADRVDVGYAEEERRCGFAAESRYHGVTYIPFAMGTNTLF
                     DVGRMVVGWVSFSPRLTPGKPVRVIVRTAERVETEVRIGVRARRQTFTFVTPLQLRVR
                     VDGADAGVVSLPLNPLPSAPMFCEASFELPAGAVCQERPRISLYGDHAAFGYWFYQPE
                     "
     gene            complement(2374..3513)
                     /locus_tag="PK576_05500"
     CDS             complement(2374..3513)
                     /locus_tag="PK576_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61083.1"
                     /translation="MAYAKIPEKDAVLLIRALGLLISQASVYGPSHKVTQNAVRSMFT
                     ELEQAVKTHGVIEIALNGRQLLVNGSSDGITEAVSKNLADRMALHKIGGLLFQAPAVL
                     EEFLICVTLLGTPPGALAAENGFEGAMHKAGLRSIQVVTVAFQRVTGERNEEPDLSHT
                     PLPADPQTATNRMRTGAGGIAVLDLSEALVLDDTAETPQTDLDTRAAKRKKHTSSLAS
                     LLRETAALLERDGDPPVGERHQVMIDALEQIRHVLSTLTVDSERHITSLAEQVNDDRQ
                     TIASIESAARRRGIGLKLTRAELIDRYAELNQEIVQPLTVSTGVIEMLKTKRAGALTP
                     LQCDLIKMAEESIERVNQLVAYMSRISGLPESLTPDSRIISESYR"
     gene            complement(3552..4673)
                     /locus_tag="PK576_05505"
     CDS             complement(3552..4673)
                     /locus_tag="PK576_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61084.1"
                     /translation="MRTRQKQGQRNGSAVVAVLGIISVIMIVSGMLARSATRQMRATQ
                     ITREMLKARLIAESGLNKAYNAVKTDFDRLNGFTETLAFGGGSCTVRAEMLTSEDGRE
                     RARLVSEGVCGLGRARVSADLENVTRVLGDDQAGSVAGDYFDLLFDLLVGGILDLKGN
                     FKATVTDIHANGNVKLTGSSNVDAMRVSSTGTIEWKKNDGTVTLLTNQSPVEILSAAL
                     TEAINAFIAVAEENGAVYAHGSDIPSSPPGGVAVCTGSSDGWSKTGTGTFIFLGDVKL
                     QGSGVNLTAQNGYPALIVLGTGEVKINANAVIRGAVIMPNASFHLNGHAVIYGAVLIG
                     QGMTGNGTADLYAGDSGQGFSLPPTHQVEQDYVVVTAWH"
     gene            complement(4716..6911)
                     /locus_tag="PK576_05510"
     CDS             complement(4716..6911)
                     /locus_tag="PK576_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE61085.1"
                     /translation="MRKGFTLLETIFAVAVFSLLLGMTLGSWLLFMYKSNRVNTQASL
                     DMDVRKVIERFRYELRNTARETVIFYPDDQEPYQAVGFALPGSGTDGLVEMAAGGTNI
                     LWRESVVYHVWNHSPHQMRRTVFANRNPNALYADRYGQIAAVVAAGNGASACLAGESA
                     STTVMFENLFTGRLWHASAQFDGYAPTPNTRDRITFGSLSLGPGEHTVSFTVKGKNKA
                     ATSQALRLDRILASVSGWPQEAEWRTTSGAATVPYHVGQNKAGAAYGLVAASVGVGDT
                     LHVTVRNDAIEECEFIGEGRNVTFSNTVVRFDTIDPPSGFAEGTYAVRIDGQFGAEKN
                     WSCAEQTGHYRNDYFKPLTNCVIRIPVMADPTRSATSGEPVLYGIKKDGYGPVFRLYK
                     SLYNGGLQVLNPSFAVVALKDVPTDYNSNLLPKLDPEERIPLEFWQDGEKKTDWDACV
                     KQRHLELRPAELIRIPMGSTLMLQFQATVTALDDRFTRFDVIRYDKPGVCLPGCWVVP
                     EGGTNLLDVFNWQGVSGLELKPFVPTLEFVAVNYADGGEYVSHPFDTKSSTGATKAFA
                     WEADVPAGADLAMFARSGDTLTDDGFGIADAPSWENVPEASNGGGFSGSTGRYVQFRA
                     AFLAQPASQFPGSGGLGGTGPYRSNTPRIRWARFTWDGEEKYVEVTAELLKNPDCGMF
                     EVKVDNKPLVHGVTMEIEIFKDVKAMSGAKQERLKSAMTAEVEPRNSGK"
     gene            complement(6926..7402)
                     /locus_tag="PK576_05515"
     CDS             complement(6926..7402)
                     /locus_tag="PK576_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE61086.1"
                     /translation="MGRTDQSGMTVVELMVAMGLLVIILTAVFPLVDQMISRFQMARD
                     HYVAASLSQGRIERARGVPYSDLHLYRELGTRVDDFGNAADPAGRFLRKTEVTVNSPT
                     TNMTHMTVTTYPCTCSRWGWRKVFHPIKTGKFICRFDELDFERMDYLFTEELKRTP"
     gene            complement(7416..8555)
                     /locus_tag="PK576_05520"
     CDS             complement(7416..8555)
                     /locus_tag="PK576_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE61087.1"
                     /translation="MSTNDKSQTIKIDIRSLGYGRSTVADRSGVEVQSDYFGKLDYRF
                     LFESAYDAILLADRHGIVLEANARATSFFQYGERERGLTGMPVETLVAGMTSSLLEEI
                     LTAITESRYMRIQAFAVRRDGSLTAVEIVAMGNGLRVPEPVCYLIRDIQSRWQAEQRL
                     LSVYHAMDNTDSGIGITDMEGKVVYANRMMVHLLAGGDETEVFGVNLSTWFHRKAVIM
                     PMLENIRQRKSWIGEQRLLVNEQAHWLQIAAVPDINDDNELRGMVVSLRDTAERRRAE
                     IAESQVERNRVMMESLAGVCHSLGQPATVLLTSIEMLKMGGVKDEKARQEIINLCYDA
                     VIQLRDLLQKMNAKRAYAVEPYLPGQEDTKDMLRIEEDQVPSRQT"
     gene            8901..9917
                     /locus_tag="PK576_05525"
     CDS             8901..9917
                     /locus_tag="PK576_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61088.1"
                     /translation="MATGLSISALTRQSKPLTDIVGIEVGSGNPDGVPAVRLRKRNGR
                     FELLAAGFLPLPGTLPLDLETAQNSTPVWLLPRPFQAPYAALALSSPLGFLWHAAETG
                     EETPDKHATPFRTASRASTPESPALLTGLPEFQAAWAARLLPEGRRPTARSIQFSPAA
                     ALNALTTSPDFKTVSGTAIALFAFAEFTSITAFQEGQLMLYREHAAGYLHLRKVVSQH
                     MGIDMALADSLLDDTLVDPTPIIEPVLRPLFRQVEISSDFLLRRRNCKADHFFLCGVP
                     AGGRYWSSVFAQMMSHTLTPLPPLDGAEALPRSNFGAESLSPIRKQLLMTAYGAARAA
                     LEDA"
     gene            9914..10561
                     /locus_tag="PK576_05530"
     CDS             9914..10561
                     /locus_tag="PK576_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61089.1"
                     /translation="MSNVYHLNLLQPEEHLSSSPIRLRVMLPMFALLSALGVGVWWSV
                     LAGRVHAVTQQKSAIEANLAQLKPTHETVLGLRTEQHDLTATLEQLAYYTRAQLRFGP
                     SLQALAHHVPDQIQLTELHIPPPPEPPPPDPKNPVWGPTNPIERVTLRLTGRTTGASQ
                     VETLLRAFRTEAFTNLIRSAQIPKGAYRQDTTRRGAAKETLLFELNCECALRRFE"
     gene            10558..11169
                     /locus_tag="PK576_05535"
     CDS             10558..11169
                     /locus_tag="PK576_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61090.1"
                     /translation="MSLNTSIIHKIAPSMQRSILITLGGLMIAGAMVFFLVLPARARL
                     QTLAAELNTLRDTLATVRADIAGTEQQRLKTAEVRSKHDALVRSGVLQPLLNSFAMRG
                     KSLLDPLAQQTGFAIANVKEDRFLRLQVPKQVPLHLYGRQMIEFSGQGAYTSIVNFIA
                     LAEERFPLCTLAGLKIISQNQTPETHKAFITFEWPAKGEKRTP"
     gene            11268..11720
                     /locus_tag="PK576_05540"
     CDS             11268..11720
                     /locus_tag="PK576_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61091.1"
                     /translation="MSVLALFLLAALPARAARDPFWPIGYEPPKPEPVVKEIPEVPKK
                     TVPKPVPPKPKPPAVKPVTDGDWTDARAFITVTGFTRATRPDTGETRTLALINRRSYA
                     AGDTLCITNAGIVFLWRIDSLDNRELKLAPIQATRIAPPVSGPSGSTP"
     gene            11782..13566
                     /locus_tag="PK576_05545"
     CDS             11782..13566
                     /locus_tag="PK576_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOE61092.1"
                     /translation="MNRITRLCAFIATGLCLAWQPVTCAQEATAQTPQPPKTTASVRV
                     TDASSPSEPLVSMTFDEAPLADVIKAFRDATGANIISGGTNVLQANISVSLDNVPWRK
                     GLSSILDPQGLQLVEQPTGSGIYVINTKTVEVPKITRVFSLEYIKAEDVADLLKTTLG
                     TTGGIVTPFKESNAVILTATEQQISECESIIKAIDKASPQVYIEVRFVELTAQASRKL
                     GLKWDSLGTKEGWGAHADPTFFNFENDYLKTKDKGMDRSQNSERVQPGDTTWRDSLTR
                     TLNRNDSSTQTRTFTGTLSLDAFSLTMNAFEQMDGVSIFSNPKIIVANEKEALVDMTT
                     KEPNLTVTSSRTGTAGDQLDISAELEVIPGEKGDKDGKGKGIFAGEAFFSYGITLKVT
                     PRISSTGLITLNVEPTISDRKSYYEFSGIEETKTPTPKYPIIYMQRLQTVFTMQDGST
                     AVIGGLTTTTEDNIDSGIPWLRELPWVGPRLFGWKSRRKEQKEIIIFVSVGLADPLKL
                     PESVGMPKNAILGRDLLTGELKEPGDRTKEEVMSLRDPVHGTKAQPSTEEVAVPAAVA
                     PDVVVNEKEAAKPVTQQQPVDTAPLLQN"
     gene            13707..15182
                     /locus_tag="PK576_05550"
     CDS             13707..15182
                     /locus_tag="PK576_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOE61093.1"
                     /translation="MATLDRFLIPLVTKSGSDLHLSAGRPPVMRIHGTLAPVPDEPVL
                     TSMQILELLREIMPTQNIEELEREWDTDCAYEIPGTARFRVNGFRDMYGFGAVMRVIP
                     ELIPTFEELNLPEVLRDFCKLSKGLVIVTGPTGSGKSTTLAAMVDYINRTRNEHIITI
                     EDPIEFVHTPRQCIINQREVHRNTRSFTRALRAALREDPDIVLVGEIRDLETMEIAIE
                     TAETGHLVLATLHTNTAATTVERMIDKFPAERQNQIRSMLSDTLKGVVAQTLCKKIEG
                     GRIAVFEVLVVTTPVAALIREAKTHMLPSVMQTGRATGMQTFSDELTRLAAKGIISAE
                     DAYIKAVDKVEIETKFRLAGISLDFKKQAEEAARIARMAKAEEMLQAGRAALQVDPKN
                     IAALCDVAWILATSPFAELRDGKEAVKLAEKACSLSHNKDAAALEVLGAAQAEYGSFR
                     RALDATRRAAEFYKQAGDVVRATALGNRLGMFEQNKPYRDE"
     gene            complement(15211..16620)
                     /locus_tag="PK576_05555"
     CDS             complement(15211..16620)
                     /locus_tag="PK576_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE61094.1"
                     /translation="MALNFWFGKKEAKSDQAMVGGVLLGTVGPVVLSEASADASASEA
                     PVPEASCPVAAGGPRAEPVVASVPVSDVPGLAPAALRSSSAEASAGSKVVLKAVGGGG
                     GVVRLRTPGTPAVAPAASGPAAAAPGAFGLQPRGGQPTAPEQAGAAPVPVASPAPPDR
                     AADTRQGTGVVRLRAPETPAGTPVASGPAAIKPGVFGLRPRGGQPAASEQAVAAPAPA
                     ASPAPPEKAADTRQGTDVVRPKSDQRALYYQLMNGLYDALLILDDQGHVVDCGRRTEE
                     LLGYTREEAWDLPVGKIVPGMTSQMFEHLKQNLAENHHILIDARCVRKDGSSFAGEVG
                     VSTLSLTRGTNMVFAVRNVERRKKAMEELRKSRAALDSSLAPTFVCDPEGLFLIVNRA
                     LLEAFGIPDEEQAKSLRFMDLLPDASRFFLRAVCGEKVRETLKVMLPNGASLKLELTL
                     QPVQSGQNVTAVAGSLLQA"
     gene            16741..>16967
                     /locus_tag="PK576_05560"
     CDS             16741..>16967
                     /locus_tag="PK576_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE61095.1"
                     /translation="MKKKRYTKNMLVRELAFSTGITQKTARFVLEALRHIAYRETADQ
                     GFTLPGLCRMDIIRRKARRMRNPQTGENILIG"
BASE COUNT         3483 a         5854 c         4611 g         3019 t
ORIGIN      
        1 cgatcgtcgc gggtcgaatg tcgcgggtcg cgggtcgacg atcccgatac cgataccgat
       61 accgaccccg ataccgatcc cgaccccgac cccgatttgg atgaaaccct gaaaccatga
      121 aaccagaaac cagaaaccat gaaaccatga aacaatcgat cccgataccg ataccgaccc
      181 cgaccccgat ttggatgaaa cccagaaacc atgaaacaat cgatcccgat cccgaccccg
      241 accccgattc ggatgaaacc ctgaaacccc gaaaccatga aaccaaagag cgtcggaagt
      301 tcacctgctg ccctcattcg ggctggtaga accaataccc gaaagccgcg tgatccccgt
      361 agagggagat ccggggccgt tcttggcaca ccgccccggc aggcaactcg aatgacgctt
      421 cgcagaacat gggcgcggac ggcagggggt taagcggcaa cgagaccaca cccgcatcgg
      481 caccatccac ccgcacgcgg agttgcagcg gagtgacaaa ggtgaaggtc tgccgtctgg
      541 cgcgcacgcc gatccgcacc tcggtttcca cgcgttcggc ggtccgaacg atcacgcgca
      601 ccggtttgcc cggcgtcagc cgcggcgaga aactcaccca ccccaccacc atacggccca
      661 catcgaacaa ggtgttcgtg cccatcgcaa aggggatgta ggtcacgcca tgatagcggc
      721 tctccgccgc aaacccgcat cgccgctcct cctccgcata gccgacatcc acccgatccg
      781 ccaggcgcca gccccggact tccttgtccg ccagatgcgg cgcgcgcgcg ccatcgagcg
      841 gagaccacac ggcttgccgc atctggttct catccacgcc caacgcggcc accgggccgc
      901 acagcacggc gatgtcggaa ccgccgaatt cggtttgcgc ggagggcagc gcccaataat
      961 cgaaccggcg ttcgggatgg tgcttgagcc gttccagatt cgtcacaact tcgggcgtca
     1021 tccaatccgg cgaatagagt ccggaaagat gagcgtaact ccgtcccggc atcccgtagg
     1081 cgagattgca gaccgccccg ccgacccgcg ccccgggcgg cagccgcgca tgcgcctctt
     1141 tggcgaattc gtaacagggg tgggtgatca ggcaggccgt atggtatagg gtgaccagcc
     1201 aggccgcgtt caacatctga aacgcggcca cgatcagcaa gaggccccag cgacaaaccg
     1261 cggtccggcc ccatccccgt ccgacccgca tagcacccgc agcggtatac accagccaca
     1321 ccggcatcag ccatgccaga tagcggtcca cattggtgtt ctgccatccg ctcgaagcaa
     1381 ccacccccac cccggccgca caggcgctca gccaccatac ctctttccag agcccctcgc
     1441 gcctccgggg taaggtcacc agcccgatcc acgcgcaaag cgccccgatc agcggcacat
     1501 aaaaactttc gcgcggcaga gcatcgggca ggccgagcaa caactcgcgc aggatccgca
     1561 ccatgtcgcc cagcgtcaag tgcagggccg aaaagaaatc gtactgctta gcataccctt
     1621 tgtgaatcac cgacaggaac tgggcgtgcc ccgtgagtcc gtaattgaat gcgaaaaggc
     1681 cggcggagga gaggagcccc accacggccg cgaccccctc ctgacgcgtc accctttgac
     1741 gcatgcccca ccgcttgaac gccagagccg cggcaaacag tacggagagg agcacccctt
     1801 cgggacggca ccacggcgac aggcccagca gcaccgcgaa accggccatc ctcccgctca
     1861 gcaaggtgaa gaggagtccc gaactcaacg ccatgaaaag cccggcatcc gattgcgaca
     1921 acgcgctgta agcggcctgg ccgctcagcg ccaccagcgc gcaggccgcg aaccggttac
     1981 gcggggaacg gatttgccgt acgatgatcc cggcccaact cacgaggaac aagagataca
     2041 acagggcgtt cagcgcgaat ccggcagtca ggagcgcgtc gcccttggcc ccgagtttat
     2101 aaagcaaacc cagcgcaaag gggtagaggt gcgaggtgct gcccgtggag gggcggtcgc
     2161 ccggcgtata gacgaaaggg tgcccctccg ccacctgtcg cgcggactgg cagtagagaa
     2221 gggtgtccgg ctggggcgcc ggcagatgaa aaccgtccgt cgccaacgag ccggccacat
     2281 aataaccggc gatctgcaac agcccgcaca ccaccatccc cttggccaga ttatttttat
     2341 gaacccaaga tccgatgctc atttttcatt ttgtcagcgg tacgattccg aaatgatacg
     2401 cgagtccggt gtgagggatt cggggaggcc cgagatccgg ctcatatacg ccaccagctg
     2461 attgacacgt tcaatgctct cctccgccat cttgatcaga tcacactgca agggggtcaa
     2521 cgcgcccgcg cgtttcgttt tgagcatttc gatcacaccg gtcgagaccg tcagcggctg
     2581 cacgatctcc tgattgagtt cggcgtagcg atcaatcagt tcggcacggg tgagcttcag
     2641 accgataccg cggcggcgcg cagccgattc gatgctggcg atggtctgac ggtcgtcatt
     2701 cacctgttcg gccagcgagg tgatgtggcg ttccgaatcg accgtcagag tcgagagcac
     2761 atgacggatc tgctccagtg cgtcgatcat cacctggtgg cgctcgccga cgggcggatc
     2821 cccatccctt tccagtaatg ccgcagtctc gcgcaacagg gaggccagag aggacgtgtg
     2881 ctttttacgt ttcgccgcac gcgtgtccag atcggtctgc ggcgtttccg ccgtgtcgtc
     2941 cagcacgagg gcttccgaca gatccagcac ggcgatgcct ccggccccgg tccgcatccg
     3001 gttcgtggcc gtctgggggt ctgcaggcag cggcgtgtgg gatagatcgg gctcctcgtt
     3061 tctttccccc gtgacgcgct gaaacgcgac cgtcaccacc tgaatgctcc gcaggccggc
     3121 tttatgcatc gcgccttcga acccgttctc tgcggccagc gcgccgggcg gcgtacccaa
     3181 caacgtcacg cagatcaaaa actcctccag cacagcaggc gcctgaaaaa gcaagccgcc
     3241 gattttgtgc agggccatgc gatccgccag attcttgctg accgcttccg tgataccgtc
     3301 ggacgagccg ttgaccagaa gttgccgccc gttcaacgcg atctcgatca cgccgtgggt
     3361 tttgaccgcc tgctccaatt cggtgaacat gctgcgcacc gcattctggg tcaccttgtg
     3421 ggaaggcccg tacacgctgg cttggctgat cagcaacccc agcgcccgga tcagcagcac
     3481 tgcgtccttc tccggtattt tcgcatacgc catgccttat cgccctctcc cgcttcccac
     3541 aagcagccgc cttaatgcca agccgtcacc accacatagt cctgctctac ctgatgcgta
     3601 ggcggcaagc tgaacccttg tccactatcc ccggcataca gatccgccgt gccgtttccg
     3661 gtcatcccct gaccgatcaa gacggccccg tagatcacgg catggccgtt cagatggaaa
     3721 ctcgcgttcg gcatgatgac cgcgccgcgg atcacggcat tggcgttgat cttgacttca
     3781 cccgtaccca gcacaatcag ggcagggtat ccgttctggg ccgtcagatt caccccgctt
     3841 ccctgcaatt tgacatcccc cagaaagatg aacgtgccgg ttccggtctt gctccacccg
     3901 tctgaagaac cggtgcagac cgccacgccc ccggggggcg aggagggaat gtccgaaccg
     3961 tgcgcgtaaa ccgcgccgtt ctcctccgca accgcgatga acgcattgat cgcctcagtc
     4021 aacgccgcac tcaagatctc gaccggcgac tggttggtaa gcaacgtgac cgtaccgtcg
     4081 ttcttcttcc actcgatggt gcccgtgctg gacacccgca tcgcatccac gttcgaactc
     4141 cccgtcagct ttacgttacc gttggcgtgg atatctgtca ccgtggcctt gaaattgcct
     4201 ttgaggtcca gaatcccgcc gaccagcaga tcgaagagca agtcaaaata atcgcccgcc
     4261 acactgcccg cctgatcgtc ccccaacacc ctcgtcacat tctccagatc ggctgaaacg
     4321 cgcgcgcggc cgaggccgca aaccccctcg gagacgagcc gcgcccgctc gcgcccgtcc
     4381 tccgaagtca gcatctcggc cctgacggta caggacccgc cgccgaaggc taaggtttca
     4441 gtaaacccgt tgagccgatc gaagtccgtt ttcacggcgt tataggcctt gttcaggccc
     4501 gactccgcga tcagccgcgc cttgagcatc tcgcgtgtga tctgcgtggc gcgcatctgc
     4561 cgcgtcgccg agcgggccag catgccggag acgatcatga tgacgctgat gatcccgagc
     4621 acagccacca ccgccgaacc gttacgttga ccctgttttt gccgtgtcct catgccgcac
     4681 ctccctgcgc cgtcgcgcgc gatcgtgttc cttacctact tgccgctgtt gcgtggctcg
     4741 acctccgcgg tcatcgcgga tttcagccgc tcctgtttgg ctccgctcat agccttgaca
     4801 tccttaaaaa tctcgatctc catggtcacg ccgtgcacca aaggtttgtt atcgaccttg
     4861 acttcaaaca taccgcaatc cgggtttttc agcaactctg ctgtgacctc cacatacttc
     4921 tcctcgccgt cccaggtgaa ccgggcccag cggattctgg gcgtgttgct gcggtaggga
     4981 cccgtgccac ccaacccgcc gctgccggga aattggctgg ccggttgggc gagaaacgcc
     5041 gcgcggaact gcacataacg cccggtactc cccgagaacc cgccgccgtt ggaagcctcc
     5101 ggcacattct cccaagacgg ggcgtccgcg attccgaacc catcgtcggt cagcgtatcg
     5161 cccgagcgcg cgaacatcgc gagatccgcg ccggccggca catccgcctc ccacgcgaag
     5221 gctttggttg cgccagtcga gctctttgta tcgaacggat gcgagacata ctccccgcca
     5281 tccgcataat tcaccgccac gaactccagc gtcggcacga agggtttgag ttcgagcccc
     5341 gacacgccct gccagttaaa tacgtccagc agattcgtcc cgccctccgg caccacccag
     5401 caaccgggga ggcacacgcc gggcttgtca taacgtatca catcgaagcg cgtgaaacgg
     5461 tcatccagcg cggtgacggt cgcctgaaac tgcagcatca gcgtgctgcc catgggaatc
     5521 cgaatcaact ccgccggacg cagttccagg tgacgctgct tgacacaggc atcccagtcc
     5581 gtctttttct ccccatcctg ccagaactcc aagggaatcc gctcttccgg atcgagtttg
     5641 ggcagcaggt tgctgttgta atcggtgggc acatccttca gcgccaccac cgcgaacgag
     5701 gggttcagca cctgcaatcc accgttatac agcgacttat agagccggaa gaccggtccg
     5761 tacccatcct tcttgatccc gtaaagaacc ggctcgcccg atgtggcgct ccgggtcgga
     5821 tccgccatga ccgggatccg gatcacgcaa ttggtcaggg gcttgaaata atcgttgcgg
     5881 tagtggccgg tctgctccgc acaactccag ttcttctccg cgccgaactg gccgtcgatc
     5941 cggaccgcat aagttccctc cgcaaacccc gagggcggat cgatggtatc gaaccgcacc
     6001 acagtgttcg agaaggtgac gttccgtccc tcgccaatga attcgcactc ttcgatggca
     6061 tcgttccgca ccgtgacatg aagcgtgtcg ccgaccccca cactggccgc gacgagtccg
     6121 taggccgcgc cagccttgtt ctggcccacg tgatagggca cggttgcggc accggaggtc
     6181 gtgcgccact cggcctcctg cggccaaccg ctcacactgg ccagaatccg gtcgagccgc
     6241 agggcctggg acgtcgcggc tttgttcttg cccttgacgg taaaactcac cgtgtgttcg
     6301 ccgggcccga gcgacagact gccgaaggtg atgcgatccc gcgtgtttgg ggtcggcgcg
     6361 tatccgtcga actgcgccga cgcatgccag agcctgccgg tgaagaggtt ctcgaacatc
     6421 acggtggtcg aggcgctctc gcccgcgaga caggcggacg cgccgttgcc cgcggccacc
     6481 accgcggcga tctgaccata gcggtccgca tacagcgcat tcggattgcg gttggcaaag
     6541 accgtgcgcc gcatctgatg gggcgagtgg ttccagacgt ggtagaccac actttcgcgc
     6601 cagaggatgt tggtgccgcc cgccgccatc tccaccaatc catccgtgcc agagcctggc
     6661 aaggcaaaac ccaccgcctg atagggctcc tgatcatcag ggtagaagat cactgtctcg
     6721 cgggcggtgt tgcgcaactc atagcggaaa cgttcgatca ccttgcgcac atccatatcc
     6781 agcgacgcct gggtgttgac ccggttcgac ttgtacatga acaacagcca ggaccccagg
     6841 gtcataccca gaagcaggct gaacaccgcg accgcgaaga tcgtctccag taatgtgaac
     6901 cctttgcgca tcacacacct cgcatctagg gcgtccgttt caactcctcg gtgaacaggt
     6961 aatccatccg ctcgaaatcc aactcatcga accggcagat gaacttcccg gttttaatcg
     7021 gatgaaacac tttgcgccag ccccagcggg agcaggtgca gggataggtt gtgaccgtca
     7081 tatgcgtcat gttggtggtc ggcgaattca ccgtcacctc cgttttccgg aggaagcgtc
     7141 cggccggatc cgccgcattg ccgaaatcat ccacccgcgt gccgagttcg cggtacagat
     7201 gcagatccga atacggcacc ccgcgcgccc gttcaatccg gccctgggag agcgatgcgg
     7261 ccacgtaatg gtcccgcgcc atctggaaac gcgagatcat ctggtcgacc aacggaaaca
     7321 ccgccgtcag gataatgacc agcaggccca tcgccaccat cagttcgacc acggtcatgc
     7381 cgctctgatc tgttctgccc atacatcgcc ccccgtcagg tttgccgcga cggcacctga
     7441 tcctcttcga tccgaagcat gtccttcgta tcctcctgcc caggcaggta cggttccacc
     7501 gcatatgccc gtttggcatt catcttctgc agcaggtccc gcaactggat cacggcgtca
     7561 taacacaggt tgattatttc ctgccgcgcc ttctcgtctt tgacgccgcc catcttaagc
     7621 atttcgatgc tggtcagcag caccgtggcg ggctggccca gcgaatggca caccccggcc
     7681 aagctctcca tcatcacgcg gttgcgctcc acctgactct cggcgatctc ggcgcgccga
     7741 cgctccgccg tgtcgcgcag cgagaccacc atgccgcgca gttcattgtc atcgttgata
     7801 tccggcaccg ccgcgatctg aagccagtgc gcctgctcgt tgaccagcag ccgctgttcg
     7861 ccgatccagc ttttgcgttg gcggatgttt tcgagcatcg gcatgatcac ggctttgcgg
     7921 tggaaccagg tcgacagatt gaccccgaac acctcggtct catccccgcc cgccagcaga
     7981 tgcaccatca tgcggttggc gtaaaccacc ttgccctcca tatccgtgat gccgattccc
     8041 gaatcggtgt tatccatcgc gtgatagacc gagagcagcc gctgctcggc ctgccaccgc
     8101 gactggatgt cgcggatgag ataacagacc ggctcgggaa cgcgtaaccc gttacccatg
     8161 gccacgatct ccacggcggt gaggctgccg tccctacgca ccgcgaaagc ctgaatacgc
     8221 atgtatcggc tctccgtgat cgccgtcaga atttcctcca gcaacgaaga ggtcatcccg
     8281 gccaccaacg tctccaccgg cataccggtc aggccccgtt cccgttcgcc gtactggaag
     8341 aaagaggtcg cgcgggcgtt cgcctccaag acgatcccgt gccggtcagc cagcagaatc
     8401 gcatcgtaag cgctctcgaa cagaaaccgg taatccagct tgccgaaata atccgactga
     8461 acctcgaccc ccgagcggtc ggccacggtg gaacgtccgt aacccaacga gcggatatca
     8521 attttgatgg tctgagattt gtcattcgta ctcatcgtaa tcgacacctc tgcgttacaa
     8581 tccgttctgt accgatcccg tttttcggct gaattacggt tccgaaccgt ttgctaaaaa
     8641 tccgctgatg agtttacatc atattttagc aaaaatcatg ccgaacccca taccccatat
     8701 ttggcacgcc gcaaacaatc tttttctccc ttctgcaaag ccgctttgca aaaacgagaa
     8761 tccgaaccgt tgccggaacg gcgtttcacg ctcggtggca gaacccccgg aagagcgcgt
     8821 ggaacatgcc ccatccggcc tgagacggca tcccgtacct ttatcgcaaa actggcattg
     8881 tttctgctta ctttacccgt atggccacgg ggttatccat atccgcgcta acgcggcaat
     8941 ccaagccgct caccgatatt gtggggatcg aggtgggttc cggcaatccg gacggcgttc
     9001 ccgcagtgcg gttgcgcaag cgcaacggcc ggttcgaact gctggccgcc ggattcctcc
     9061 ctctgccggg aaccctgccc ttggatctgg agaccgcgca gaattccacg ccggtctggt
     9121 tgctgccgcg tccgtttcag gcgccgtacg ccgccctggc gctctcctcg ccgctcggat
     9181 tcctatggca tgccgcggaa acgggggagg agacgccgga caagcatgcg acccccttcc
     9241 ggaccgcctc ccgggcctca accccggaat ccccggcgtt gctgaccggc ctgcccgagt
     9301 tccaggccgc ctgggcggcc cgtctccttc ccgaaggccg ccgccccacc gcccgctcga
     9361 tccagttctc cccagccgcc gcactcaatg cgctgaccac ctcgccagac ttcaaaacgg
     9421 tttccggcac ggccatcgcg ctctttgcct tcgccgagtt cacctccatc accgcctttc
     9481 aagaggggca actgatgctg taccgcgaac atgccgcagg ctacctgcac ctccgcaagg
     9541 ttgtcagcca acatatgggc attgatatgg ctctggcgga ctctctgctc gacgacacgc
     9601 tggtcgaccc cacgcccatc atcgagccgg tgctccgtcc gctgttcagg caggtcgaga
     9661 tttcctcgga ctttctgctc cgcagacgga actgtaaggc ggaccacttt ttcctgtgcg
     9721 gcgtgcctgc cgggggccgt tactggtctt cggtgttcgc gcagatgatg agccatacgc
     9781 tcacgccgct cccaccgctg gacggggccg aagccctgcc ccgctccaac ttcggcgcgg
     9841 agtccctctc gccgatccgg aaacagttgc tgatgaccgc gtacggtgca gcgcgcgccg
     9901 ccttggagga cgcatgagca acgtatacca cctcaatctg ctgcagccgg aagagcacct
     9961 gagttccagc cccatccgtc tccgggtgat gctcccgatg ttcgccctgc tgagtgcgct
    10021 cggcgtgggg gtctggtgga gcgtgctggc gggccgcgtc catgcggtca cccagcagaa
    10081 aagcgccatt gaagccaatc tggcgcaact gaaaccgacg cacgagaccg ttctcggatt
    10141 gcgcaccgaa caacatgatt tgacggcgac gctggagcag cttgcctatt acacgcgggc
    10201 gcaactgcgc ttcggccctt cgcttcaggc gctcgcgcat catgttccgg accagatcca
    10261 gctgaccgaa cttcatatcc cgccgccccc ggaaccgccg ccgcccgacc ccaaaaaccc
    10321 ggtctggggc cccaccaacc cgatcgagcg cgtcaccctg cgcctgacgg gacggaccac
    10381 cggtgcctca caggtcgaaa ccctgttgcg cgccttccgt accgaggcct tcaccaacct
    10441 gatccgttca gcgcaaattc ccaagggcgc ctaccgtcag gacacaaccc gacgcggtgc
    10501 ggccaaagag acgctcctgt ttgagttgaa ctgcgaatgc gcactcagga ggttcgaatg
    10561 agcttaaaca cctccataat ccataaaatc gccccctcca tgcaacgctc gattctgatc
    10621 accctcggcg ggctcatgat cgccggtgcg atggtcttct tcttggtgtt gcccgcgcgc
    10681 gcgcgcctcc aaacgctcgc cgctgaactg aacaccctcc gcgacaccct cgccaccgtg
    10741 cgcgcggata tcgcaggcac agagcagcaa cggctgaaga ccgccgaggt ccgctcgaaa
    10801 cacgacgccc tggtccgatc gggtgtgctt cagccgctcc tcaactcgtt cgccatgcgg
    10861 ggcaagtcgc tgctcgatcc gctcgcccag caaaccgggt ttgcgatcgc caacgtcaag
    10921 gaagaccgtt ttctgcgact gcaagtgcca aaacaggtgc ccctccatct ctatggacgc
    10981 cagatgatcg agttctccgg gcagggggcc tacacctcaa tcgtgaactt catcgcattg
    11041 gctgaagaac ggttccccct ctgtaccctc gcgggactca agatcatcag ccaaaaccag
    11101 actccggaga cgcacaaggc tttcatcacc ttcgaatggc ccgccaaagg cgagaaacga
    11161 accccatgag aaacgatcag cttccaactc tcaacgctca acgctcaacg actcttcccc
    11221 gggagccggg tgctcggtat ttgacgttaa acgttgaacg ttgcgtgttg agcgttcttg
    11281 cccttttcct gctcgccgcc ttgccggctc gcgccgcgcg cgaccccttc tggcccatcg
    11341 gctacgaacc gccgaaaccc gaacccgtgg tcaaggaaat ccccgaagtg ccgaaaaaga
    11401 cggtgcccaa acccgtcccg cccaaaccca agcccccggc cgtgaaaccc gtgaccgacg
    11461 gagactggac cgacgcgcgt gccttcatca cggtcaccgg gtttacccgc gcgacacgcc
    11521 ccgacacagg tgaaacccgc accctggctc tgatcaaccg ccgctcctac gcggccggcg
    11581 acaccctttg catcactaac gccggcatcg ttttcctctg gcgtatcgac tcccttgaca
    11641 accgcgaact gaaactggct ccgatccaag ccacccggat cgccccgcct gtgtcgggac
    11701 cctccggaag cacaccctga cgcgattttt tccatgtctt ccatcactgt cgattcacaa
    11761 cccaaaacca ggagtatcca catgaaccgt atcacccgac tctgtgcgtt catcgcaacc
    11821 ggcctgtgtc tggcctggca gcccgtcaca tgcgcccaag aggctacggc ccaaacgccg
    11881 cagcccccca aaaccaccgc ctcggtccgc gtcaccgatg cctcatcccc cagcgagccg
    11941 ctcgtctcca tgaccttcga cgaggcgccc ctcgcggatg tgatcaaggc tttccgtgac
    12001 gccacggggg ccaacatcat ctccggcggc accaatgtgc tgcaggcgaa catcagcgtc
    12061 agtctcgaca atgtgccgtg gcgtaaaggg ctctcctcga ttctggaccc tcaaggcctg
    12121 caactggtcg aacagcccac cggatccggc atttacgtga tcaacacgaa aaccgtcgag
    12181 gtccccaaga tcacccgcgt gttctcgctt gagtacatca aggccgagga tgtcgccgac
    12241 ctgctgaaaa ccaccctcgg caccaccggc gggatcgtga ccccctttaa agagtccaat
    12301 gcggtcatcc tcaccgccac ggaacagcag atcagcgagt gcgaatccat catcaaggcc
    12361 atcgacaaag cctcgccgca agtctacatc gaggtccgtt tcgtcgagct gaccgcgcaa
    12421 gccagtcgga aactaggcct gaaatgggat agcctcggca ccaaagaagg atggggtgcc
    12481 catgccgacc ctaccttctt caacttcgag aacgattatc tcaaaacgaa agacaaaggt
    12541 atggaccgca gccaaaattc cgagcgtgtc cagccgggtg acacaacgtg gcgggattcc
    12601 ctgacccgca ccctcaatcg caacgacagc tccacccaga cgcgcacctt taccggcacc
    12661 ctctcgctgg acgccttctc cctcaccatg aacgcgttcg aacagatgga cggggtttcg
    12721 atcttctcca atcccaagat cattgtcgcc aatgaaaaag aggcgctggt ggacatgacc
    12781 accaaggaac ccaacctgac cgtgacctcc tcccgtacgg gaaccgccgg cgaccaactc
    12841 gacatctcgg ccgaactgga ggtcatccct ggcgagaaag gggacaagga tggcaaaggc
    12901 aagggcattt tcgcgggtga ggcgttcttc agctacggta tcaccctcaa agtcacgcca
    12961 cgcatcagct ccaccggtct gatcaccctg aacgtggaac ccacgatcag cgatcgtaag
    13021 agctattacg agttttccgg cattgaggag accaagactc ccaccccgaa atacccgatc
    13081 atctacatgc agcgcctcca gacggttttt accatgcagg acggttccac ggccgtgatc
    13141 ggcggcctga ccacgacaac cgaggataac atcgattccg gcatcccctg gctgcgtgag
    13201 ttgccttggg tgggaccacg cctcttcggc tggaagagcc gcaggaaaga acagaaggaa
    13261 atcattatct tcgtctcggt cggtctggcc gaccccctca agttgcctga gtccgtcggt
    13321 atgccgaaaa acgccattct cgggcgcgac ctgctgaccg gtgaactaaa agagccgggc
    13381 gaccgcacca aagaagaggt gatgagcctc cgtgatcccg tgcacgggac caaagcgcaa
    13441 ccatccacag aagaggtcgc cgtacccgcc gcagtcgctc cggacgtggt ggtgaacgaa
    13501 aaagaggcgg ccaaaccggt aacacaacaa cagccggtcg ataccgcccc gctactgcaa
    13561 aattagaagg gaacctgtca gaacggtccc ggcggcgcag gccctcccgc gccgccggga
    13621 acaaaggtcc gcttcaccga agcctcccag gctgaatccc tgttgatgtt gtaaaccccc
    13681 gaaccgcact ccccgtcagg actcccatgg ccacactcga ccgcttcctc attccgctcg
    13741 tcaccaagtc aggcagcgac ttgcacctca gcgcgggccg tccgcccgtc atgcggatcc
    13801 acggcaccct ggcaccggtt ccggacgaac cggtcctgac cagcatgcag atcctggagt
    13861 tgttgcggga aatcatgccg acacagaaca tcgaggaact cgagcgcgaa tgggacaccg
    13921 actgcgccta cgagatcccg ggcacggcgc gcttccgcgt caacgggttc cgcgacatgt
    13981 acggattcgg cgccgtgatg cgcgtgatcc ccgaactgat cccaacgttc gaggaactca
    14041 acctgcccga ggttctgcgc gatttctgca agctctccaa agggctggtc atcgtcaccg
    14101 gccccactgg cagcggtaaa tccacgactc tcgcagctat ggttgattat atcaaccgga
    14161 cacgcaacga acacatcatc accatcgagg accccatcga gttcgtgcac acgccgcggc
    14221 agtgcatcat caaccagcgt gaggtgcacc gcaacacacg cagtttcacg cgggcccttc
    14281 gcgccgctct gcgcgaagat ccggacatcg tgctcgtggg cgaaatccgt gatctggaga
    14341 ccatggagat cgccatcgaa accgcggaga ccggccatct ggtcctcgcc accctgcata
    14401 ccaacactgc agccaccacc gtagagcgca tgatcgacaa gttcccggcc gagcgccaga
    14461 accagatccg ttccatgctc tccgacacgc tcaaaggcgt ggtggcgcaa accctctgca
    14521 aaaagatcga gggtggccgg atcgcagtgt tcgaggtgct ggtcgtcacc acgccagtcg
    14581 ccgcgctcat ccgcgaggcc aagacgcaca tgctcccctc ggtaatgcag accgggcggg
    14641 ccacgggcat gcagaccttc agcgacgagc tgacgcggct cgcggccaaa ggcatcatct
    14701 cagccgaaga cgcctacatc aaagccgtgg acaaggtgga gatcgaaacc aaattccggc
    14761 tcgcgggcat cagcctcgac ttcaagaaac aagccgagga ggccgcccgt atcgcacgca
    14821 tggccaaagc cgaggaaatg ctgcaagccg gacgcgcggc gctgcaggtc gaccccaaaa
    14881 acatcgcggc cctgtgcgat gtggcgtgga ttctcgccac gtcacctttt gcggagttgc
    14941 gtgacggcaa agaggcggtg aaactcgcgg agaaagcctg tagtctgtca cacaacaaag
    15001 atgcggccgc gctcgaggtg ctgggcgcgg cccaagctga atacggatcc ttccggcgcg
    15061 cgctcgacgc tacgcgccgc gctgccgaat tctacaaaca ggcgggtgac gtcgtacgcg
    15121 caaccgcact cggcaatcgc ctcgggatgt tcgagcagaa caaaccctat cgggatgagt
    15181 gaggcgctcg ctccggcgcg gcgcggagac tcaagcttgt aaaagcgagc cggccacagc
    15241 ggtgacgttc tgaccgcttt gcacgggttg caacgtcagt tccaacttca gcgatgcgcc
    15301 gttcggcagc atcaccttga gcgtctcccg gaccttctcg ccgcaaaccg cccgcaggaa
    15361 gaaccgtgag gcgtccggca gcagatccat gaaccgcaga cttttcgcct gttcctcgtc
    15421 cggaatcccg aacgcctcca gcaacgcccg gttcacaatc aggaaaagcc cttccggatc
    15481 gcagacgaac gtcggcgcca acgacgaatc cagcgcggcc cggcttttgc gcagttcctc
    15541 catcgccttc ttccggcgct cgacattgcg gaccgcgaaa accatattcg tgcctcgggt
    15601 cagggaaagc gtgctcaccc ccacctcgcc cgcgaatgag gagccgtctt tccgcacgca
    15661 ccgcgcgtca atcaggatat gatggttctc agccagattc tgcttcagat gctcaaacat
    15721 ctggctcgtc atacccggca caatcttccc caccggcaga tcccacgcct cctcacgcgt
    15781 gtaccccagc aactcctccg tccgtcggcc gcaatccacc acatgcccct gatcatccaa
    15841 aatcagcagc gcatcgtaca gcccgttcat caactggtag tagagagccc gctgatccga
    15901 cttgggacgc accacatccg ttccctgccg cgtatccgca gccttttcag gcggagccgg
    15961 tgaagcggcg ggagccggtg cggcaaccgc ctgttccgac gccgccggct gtcccccccg
    16021 cggccgtaac ccgaacacac cgggcttgat ggcggcaggc cccgaagcca ccggcgtgcc
    16081 tgccggggtt tcaggagccc gcagacgcac cacacccgtt ccctgccgcg tgtccgcagc
    16141 cctgtccggc ggagccggtg acgcaacggg aaccggtgcg gcacccgcct gttccggcgc
    16201 cgtcggctgc cccccccgcg gctgcaaccc gaatgcgccg ggcgcggcgg cggcaggccc
    16261 tgaagccgcg ggcgcaactg ccggggttcc gggagtccgc aaacgcacca ccccaccacc
    16321 gcctcccact gccttgagca caactttgga accggcggag gcttccgcac tacttgagcg
    16381 caacgccgcc ggagccaaac ccggaacgtc gcttaccggc acagatgcaa caaccggttc
    16441 ggcacgcggc ccacctgccg cgacaggaca agaagcctcc ggaaccggtg cttcagaagc
    16501 agacgcatcc gcagacgctt ccgaaagcac gaccggaccg acggtcccca aaagcacgcc
    16561 cccgaccatc gcctgatcgg acttggcctc tttctttcca aaccagaagt tcaacgccat
    16621 gatttccttc ctgatatgct aatcagcagg atcaatgcca agacatgcct ctcaaacatc
    16681 ccatctttgg cacaacttct gctaaacaat gtaggattat tatgagtttt ccaataggcc
    16741 ttgaaaaaaa aacgctacac gaaaaacatg ctggtgcgcg aactggcgtt cagcacagga
    16801 atcacccaga aaaccgcgcg atttgttttg gaggccttgc gccacatcgc gtatcgcgag
    16861 acggcggatc agggtttcac gctgccggga ttatgccgca tggacatcat ccgacgcaag
    16921 gcgcgccgga tgcggaatcc tcaaaccgga gaaaatattc tcattgg
//
LOCUS       DAOSLE010000057        16881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3592_length_16881_cov_7.229823, whole genome shotgun sequence.
ACCESSION   DAOSLE010000057 DAOSLE010000000
VERSION     DAOSLE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16881
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_16881_cov_7.229823"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..583
                     /locus_tag="PK576_05565"
     CDS             143..583
                     /locus_tag="PK576_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE61096.1"
                     /translation="MKISTKGRYGLRILMDIALHQDAGAVTLNEIARRQGISVKYLWQ
                     VINPLKTAGLLSVTRGAKGGYMLARSPEQITVLDIVRILEGSVVLLDCLTNKKHCDQM
                     DICVARTVWMEAQNAMEDVLRGITLETVLRRCADATATANDYSI"
     gene            complement(627..1415)
                     /locus_tag="PK576_05570"
     CDS             complement(627..1415)
                     /locus_tag="PK576_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61097.1"
                     /translation="MSQKHARADESAKTDLKTIPENLPEELLPLYDWWRSKGPQSLIT
                     FGAVVLVCGAVWAFKYYRTAKIAAANQAFLQAHSLEDLEGVVAQYGSFKAGNAARLRL
                     AKAYFDAGKAQESLDLYELCLKKGAPQGFREIAELGRAHALEALNRLDDALTAYQEFE
                     RDRTDHFLQPQSVMGIARVLTLQGKKDEARKRLENLKAAKTGDATWEMAVAQLQGVVD
                     RYEPRAARSLFDAADQAAQSVPPALAVPGAVTQSAPAAVSAPEK"
     gene            1618..2886
                     /gene="guaA"
                     /locus_tag="PK576_05575"
     CDS             1618..2886
                     /gene="guaA"
                     /locus_tag="PK576_05575"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOE61098.1"
                     /translation="MKQTHDTVVILDFGAINSQAAAKLIRQLNIYCEVLPCTAARERI
                     EALRPKALVLQRGAEFGANTLKRSPATDPAAWGVPVLDLSDLPCEIATFETFLLSQCG
                     LRQDWTVEAFIREAIDEIRGMVGSHRVLLAMSGGVDSSVCALLAHRAVGDRLTCVFVD
                     HGLMRKGESDEVCSVFRDRFGIPLRHVDAAERFLARLAGITDPEHKRKIIGEEFIRVF
                     EEEAAAAGEPDFLVQGTIYPDIIESGWDGGKPVKAHHNVGGLPDRIAFKGLVEPLKYL
                     FKDEVRQVALALGIPAAIAMRQPFPGPGLGVRCLGEVTREKLDILREADFLFRNAIVA
                     ADLETQANQYFAVLTDSRSVGVSQDARTYAYTVALRAVQTSDFMTAQFVKIPLDVLES
                     VSEQITREVPQVNRVVYDITNKPPSTIEWE"
     gene            2962..5406
                     /locus_tag="PK576_05580"
     CDS             2962..5406
                     /locus_tag="PK576_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOE61099.1"
                     /translation="MQNFEKLGSFYLGKEYDLAAGARTDNLLLYDAKDLTTHAMIVGM
                     TGSGKTGLAISLLEEAAIDGIPAILIDPKGDLANLMLQFPDLTPDAFQPWVDPSEATR
                     KGVSLADLAAATAQTWRNGLVEWGQDPSRIRRLRDGAEFVIYTPGHASVRPLQILRSF
                     AAPPPALRSDDAALHDRVLTAVSGLLGLLGLDADPVQSRDAILLSNLFCHAWREGRDL
                     DLAALIRDVQKPPFARVGVLDLETFYPSAERMGLVMRLNNVLASPGFSAWMEGEPLDV
                     QRLLFSPTGKPRHAILYLAHLSDTERMFFVTLLLNEVTAWMRAQSGTGSLRALLYMDE
                     IFGYFPPTANPPSKTPMLTLLKQARAYGLGVVLATQNPVDLDYKGLSNCGTWFIGRLQ
                     TERDKKRVIEGLEGAAASSGAGFDRARTEQLLAGLGNRLFLMRNVHEEEPVLFQTRWA
                     LSYLRGPLTLPQLATLAPPRDTIPEAQPVTQTAALSGASFPTAATPLPPAAAHPLPPD
                     LPVFHLRPGASFSADLVYRPMAAGLVNMRFVDAKAGLDFWMNGIALAPFLDEGARAAW
                     EECEIRRGTSSALLDPQPLPTVAFETPPVHLPGSKQLASWAKDLESHLYQTHTFELLT
                     YPDLKLAAMPDESEGDFRARIILRLREQRDAELAKWKAAYAPKFQTLTDQIRRAEERV
                     AREKEQAGQQKFNTALAFGATLLGAFLGRGGAPVGTVGRATTAMRSAGRIRKESGDVA
                     RASENLETLRQRLSDMQEQFDRELSALQAPVAAEALSLVKRPVRPRKSDLSVGTVGLC
                     WVPWRKRPDGMFEPAG"
     gene            5475..5837
                     /locus_tag="PK576_05585"
     CDS             5475..5837
                     /locus_tag="PK576_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61100.1"
                     /translation="MSCSIPDTRTRLLLLRGVLRRFFLNLFRPAYVRDSLARRQGACN
                     RCGACCHLVANRCARLRVYPDGHSECWRYEGFRMPNCKTFPIDARDLAERDWIAPDTP
                     CGYFWPDAPVTPQPSRRS"
     gene            complement(5855..6994)
                     /locus_tag="PK576_05590"
     CDS             complement(5855..6994)
                     /locus_tag="PK576_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE61101.1"
                     /translation="MNNPIWLMTSAFPKLTFAQVVKRAKAVGAQGLELCVFRRDGSRQ
                     DHVATHLNYETFTLEDARRVLETANKEGLRFSVGAYDNLLGGDPAVCEANRNHILKLI
                     RIAHLLGGDANDVCVGTFVGYNHELGKEDGGFAKNLECYARVFRPIVRYAEDLGVTLT
                     YENCPMEGWRPASTPTTYNNLPCTLAARKLMYALIPSPAHGETYDPSHDAWQHIDPVD
                     VVRNTDLNRLRRVHIKGTRNRTNSLAAIQWGRLYPMQQVDAKLAKRAGVCQPASEWDR
                     HHYDPKLPGFGGSDSIDWFAFLKTLMELGFDRPFVIENEADNSSHTGNAAATLQGFAA
                     TVMALAPAVWPLDAEKGWCFNAKRLRPLKSAPKKDVPVVTMQDLM"
     gene            complement(7111..11928)
                     /locus_tag="PK576_05595"
     CDS             complement(7111..11928)
                     /locus_tag="PK576_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE61102.1"
                     /translation="MRSRLWVLLVPILVLMTCRGFSQSVWTGGGAGPQWSIGGNWQGG
                     AAPVPGPLTSLTFDGSVTQTVNDFQPGSFFSNLTFAASSAPFNLSGNPFILCGNLANN
                     STGRQRIAADVAWSGVSRTLDTGAGDVVLAGHLTSGAGNAAFTKNGSGRLILEGGANF
                     TAVDPVSKINGGSLIVASDAVFNRLDLQNNASLIITNNATLSGFTASYVFWQACTVDL
                     YSGRIVVGNSLLTAQDRTKTCVVNVRGGVFTAGGAPRWSNNGNTLLTLSGDGLFEWLA
                     TGQELAENGGSHVILNGGMFKFSADGAGEFDVGYRLSNPKRDGTITNRIDFNAGLFLT
                     GGFKHSAKTNLLTTLAFNGGTVRAGKTTAGFFGARPDLLDSRIQAGGLVLDTQGFAVT
                     LNDALESGATPDGGLIQLGGGTLTLAADCTYTGPTVVSNGTLTIQAAVASESLRLAPF
                     TTATVVSNGFFAGSLTVGGNARFLFTPGMTDSLSLTGMTLGDAQGAGQLVFEVTDEEN
                     TDRLDVTGPLDLSGKGDVFLYQPGTTTAFTANGTYALITFSGALTGSASDLRVANPDP
                     TKQYAFTESDGVISVTISERSGARWIADADGDWSVPGNWDGPVPNAAGAFAAFLTNAT
                     APVTVTLDAPVTVGNLVLSNNVVPYTFSGTNVLTFDSGSQPSFLSLQSGQHEMAVPWV
                     LADEMRVETAAGASLTLSDAVSGIGPMTVTGAGGVTLLGTNQTLTLLRNGFLSIADGA
                     ALAGGVTFDNGILEITQSTVLDAPLEVGLRGAGVRPATNVTLTLASESQGGGKLRQNG
                     SGALSLAAPLGNIGGVSVESGTLLFTNDVFGSMPLELAGGTARYAGSENTTLGTPLVV
                     SASSQLAADSAGLTLDGPVAFPAVATLGLDVPNGLTLAGQATLSESGRKLHLLRGTLR
                     FAAGADYTLFSGARDTLKLGQDANAATELVIEEGARLRTGGIHLNTTLADATQCVALV
                     RQEGGIVEVTDGQGLFIRDYGYSDGIYQMNGGIFTAPVSSWTSVGSKGPGYLTVNGGT
                     MTLGRFAMGVNDFSSHVWNGPGGHLAINNGRLTIAGQCSWMSDAYRSRYNTVVLGNGT
                     PESGELNLVATTRAVPYASGQGGRTAFTFNGGVLKTIGVAPYEPSSLSDYLFGVDTLT
                     LRSGGAIIETPHADVALQQPLRAAEPMGGLIKRGTAALTLAHSNNVWCGETVVQAGTL
                     RARLNQRPQRLYPEGLLALWTFDDGTSNDQSGNGFHLLQQNDTNLVSFVEGGALGKAA
                     RFTGLSSLKMAYTSVFDITSFTVSAWFKLASKGGHQGIFSGRVNAAENSTGGTFDFKY
                     NDGDYISNLIPGNSGHGAHIKPEMGGRVAIGEWHMVTYVVVPGRVDAYLDGEWKAYTN
                     GLTSALLTPGFLLTLGRGIATSDNPNREMMRDGGMIDDVAVFSRALTAAEIATLCAEA
                     VTPRPAVRVGESAVYDLLGSTNDTPSVAGGGTISNGVLAVSERLEPEGEMETATLTVR
                     HLLLDSTNLIYACTVTDTTNDLVRVSETLEVRQAGSIDLGRTELDPLSTPWRRTVMTF
                     GAIDAADAARLSRWQVTGDGITGKNILRTVIVDTAQGTVDVELRFAGTFMLLR"
     gene            complement(12106..15405)
                     /locus_tag="PK576_05600"
     CDS             complement(12106..15405)
                     /locus_tag="PK576_05600"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61103.1"
                     /translation="MDMKKNFLLVFSLAVCSGVLVAEEFRPVAVTTDAECYPDPPGKY
                     AVDKMIDNDLGTYACLLDTTRTGSDTRVYPPKAEPPVTAEFVLDLGASRTVCGMRFVA
                     RNAWVYVGPKNVSVAACADAKGQEQVRPLAGKRELPAFSNSNSAFVEWKPVSTRYLRV
                     SVHDSYQKRYPKALWMHQAQIPWSRKVGLPIAGDGQNLNVQIAEITCYDRRPADVPVK
                     NTADTAFPRHRLERDWLYQDAGLDIAQVFTSPNDAAKERAMVQRVVGCVPVDDPAAKT
                     LRQRLESLDTGRVPARDRRWRDLYLDACAARRKVRLRDIRAKADRFIYVKHGIFGCMQ
                     GLVGKYDIPDEQVRDFTPGFKRGGQLCMGTLLEDGAVKHEVLIEKPEGAICYPSLSWD
                     AQTVVFAMRDHFDTDSYYLYTMDVASRKVRQITFPIVKDGKTLHVADCEPVFLPDGRI
                     AFTSTRDVHISDCWYRAGGNIYTCNIDGSGIRRLTSDQLMTSNPGVLEDGRIVFTRWE
                     YNDRSALYLHPLIAMNPDGTSQTEYYGNNSMFPAAIIHARGIPGSHKAIAVIAGHHSI
                     YKGKLGLIDRTEGTQAGQGIEFVNTRLNGGLGRESADFIRPEPPDPARPYHDFKIDVF
                     GQFGPQYAHPFAFDEENYFCSFCPEGYLPTFGPFNPPLGLYYMHADGARELLAYDDWQ
                     STGRAVPVMRRTPPPVRPSHIMPTHNHGIYTVQDVYQGPGLQGIPRGTAKKIRVVALE
                     YRVCRMGYGQNGGECETGLCQTPISLNSGSWDVKHVLGEVDIEADGSCSFEVPARAPV
                     YFQVLDAHGYAIQSMRSWSTLQNGEHFACIGCHESKHDAGAHAASSAKTVLALAKPPQ
                     KLKSFGGREHPLIKRLKTQSWRDSVENYLGVNAPRSLDADAPVDGFSYVQEIQPIFDK
                     HCIRCHNADNLAKCKISLTGEPAKPETIKLIGAGQVDPKRAYTHSYVAITTSGNPDRN
                     PWMTWLKPRSRAVMLPPYHTGACKSKIMAHFEPGHHDVKVDDVEKRVFACWLDLLVPF
                     CGSYTQHNTWTDAEKAEFHYFQEKRRAYAAKELIGWGVTHLQSQCPSALASATRQRVP
                     QVRQQTVPSDH"
     gene            complement(15619..15975)
                     /gene="rplT"
                     /locus_tag="PK576_05605"
     CDS             complement(15619..15975)
                     /gene="rplT"
                     /locus_tag="PK576_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE61104.1"
                     /translation="MSRVTNAPVSRARRRRRLQLAKGFRGSRSKLFRQATEAVDRAQR
                     LATEHRKLRKRDFRRLWIARINAATRNEGMTYSRFIEGLTKAGITVNRKMLSEIAIAD
                     AEGFKVLIEKARAALV"
     gene            complement(15995..16192)
                     /gene="rpmI"
                     /locus_tag="PK576_05610"
     CDS             complement(15995..16192)
                     /gene="rpmI"
                     /locus_tag="PK576_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE61105.1"
                     /translation="MPTMKTKKSATKRIKMSATGKVIRSQSGKSHLNGYKSRGRKRNL
                     RGTTVAKDVFVKTAVRMLQGN"
     gene            16575..>16881
                     /locus_tag="PK576_05615"
     CDS             16575..>16881
                     /locus_tag="PK576_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOE61106.1"
                     /translation="MARITIPVEEFKQRVANAAKLVQAKGLDVLVVNGSEADYADTRY
                     FSNFWPVFERVGVAIAANGEAALMVGPESQVFADDFSWMDRICVVYQYRESANPAYPE
                     "
BASE COUNT         3264 a         5393 c         4901 g         3323 t
ORIGIN      
        1 cgtgcttcac cggcaaacgt ttttttcaca ccctccgttt cttcgtttcc gacggcgggc
       61 attttcgtgt tccgtattgg gaccgaatcc gttagaatct gcctcgttcc caaacctaca
      121 tgcgctgaaa aaggatgtaa atgtgaaaat ctcgaccaag ggacggtacg gactccgtat
      181 cctgatggac attgccctgc atcaggacgc gggcgcggtg acgctgaatg agatcgcgcg
      241 acgccaagga atctccgtga aatatttatg gcaggtgatc aatccgctca agaccgcagg
      301 gctcctgagt gtgacgcgcg gtgcgaaagg cgggtacatg ctggcgcggt cgcccgaaca
      361 aatcacggtt ctggacatcg tgcggattct agagggctcc gtggtgctgc tggactgcct
      421 cacgaataag aagcattgcg accagatgga catctgcgtg gcgcgcacgg tctggatgga
      481 ggcgcagaac gccatggagg acgtgctgcg aggcataacg ctggaaaccg tactccgccg
      541 ttgcgcggac gcgaccgcca cagccaacga ctactcgatc tgacgcaccg cgtcgggccg
      601 cggcatacga acggatcccg cctcgctcat ttttccggcg cggaaaccgc agcgggcgcc
      661 gactgtgtca cggcaccggg cacggctaac gcaggcggca cgctttgcgc ggcctgatcc
      721 gcggcatcga acagcgaacg cgcggcgcgc ggctcatacc ggtcgacaac cccctgaagc
      781 tgggcgaccg ccatctccca ggtcgcgtca cccgtttttg cggccttgag gttttccagc
      841 cgtttccggg cttcgtcctt tttaccctgc aacgtcaaca cacgcgcgat ccccatcacg
      901 gactgcggct gcagaaaatg atccgtccgg tcacgctcga attcctgata ggcggtcagc
      961 gcgtcatcca gacggttcag cgcttccagc gcatgcgcgc ggcccaactc cgcgatctcc
     1021 cggaaccctt gcggcgcgcc ttttttcagg cagagttcgt acaggtcgag tgactcctgt
     1081 gccttcccgg catcgaaata cgctttggcc agacgcagcc gcgcggcgtt tccggccttg
     1141 aacgagccgt actgcgccac cacaccctcg aggtcctcca acgaatgagc ctggaggaac
     1201 gcctgattgg cggccgcgat tttcgccgta cggtagtact tgaacgccca gaccgcaccg
     1261 caaaccagca caacggcacc gaaggtgatc agcgattgcg gacccttcga ccgccaccag
     1321 tcatacaagg gcagcaactc ttccggcagg ttctccggaa tggttttcaa atccgttttg
     1381 gccgactcgt ccgcacgggc atgcttttga ctcatgtttt ccgcctctga aaagggtaaa
     1441 aagttttagt attggggttc cgcagacgaa aagcaagacc aaagaggacg aaatcacgtt
     1501 cggaacggga atgcgtccgt gttccgcttg ttcccctgcc gtatttcctg ctatgatctg
     1561 tttttatgga tgccggggta aaccggtgtt ccgcccaaca atcggtatgg aggacccgtg
     1621 aagcagacac atgacaccgt tgtgattctt gatttcggcg ccatcaacag ccaggctgcg
     1681 gccaaactca tccggcagct caacatctat tgtgaagtgc tcccctgcac cgccgcgcgt
     1741 gaacggatcg aagccctccg ccccaaagcg ctggtgctgc agcgcggcgc agagttcggc
     1801 gcgaacaccc tcaaacgctc gcccgcgacg gatcccgctg catggggagt cccggttctg
     1861 gatctgtcgg atctgccctg cgagatcgcg accttcgaaa cctttctcct cagccagtgc
     1921 ggcttgcgtc aggattggac ggtcgaagcg ttcatccgcg aggcgatcga cgaaatccgc
     1981 gggatggtgg gatcgcaccg ggttctgctg gcgatgtcgg ggggagtcga ctcttcggtc
     2041 tgcgccctgc tcgcgcaccg tgcggtcggc gaccggctca cttgtgtgtt cgtggatcac
     2101 ggcctgatgc gcaaaggtga atcggatgag gtctgttctg tgttccgcga ccgtttcggg
     2161 atcccgttgc gccatgtcga tgcggcggag cgtttccttg cgcggctggc gggcatcacc
     2221 gacccggaac acaaacgcaa gatcatcggc gaggagttca tccgcgtgtt tgaggaagag
     2281 gccgccgcgg cgggcgagcc ggatttcctg gtgcagggga ccatctatcc cgacatcatc
     2341 gaaagcggat gggacggcgg aaaaccggtg aaagcccacc acaatgtcgg cggcctgcct
     2401 gaccgcatcg cattcaaggg gttggtggag cccttgaaat atctgttcaa agatgaggtg
     2461 cgtcaggtgg cactggcctt gggaatcccg gccgcgatcg ccatgcgcca gccgtttccg
     2521 ggccctggtc tgggtgtgcg ctgcctcggc gaagtcaccc gggagaaact cgatattttg
     2581 cgcgaagcgg acttcctgtt ccggaacgcg attgtggcgg cggatctgga aacgcaggcc
     2641 aaccagtatt ttgccgtgtt gaccgacagc cgttcggtgg gtgtcagcca ggacgcccga
     2701 acctatgcct acaccgtcgc gcttcgggca gtgcagacat cggacttcat gaccgcgcaa
     2761 tttgtgaaaa tcccgctgga tgtgctggaa tccgtctccg aacagatcac ccgcgaggtg
     2821 ccgcaagtga accgcgtcgt gtacgacatc acgaacaaac cgccgtccac gattgaatgg
     2881 gagtgacggc atacgaggcc gcgccatacg cgcctgcacc ggaaaacaat gaggggcaca
     2941 gcttgtaaca gggagcaaac catgcaaaac ttcgaaaaac tcggctcgtt ctatctaggt
     3001 aaggaatatg atctcgcggc aggggcgcgg acagacaacc tcctcttgta tgacgcaaaa
     3061 gacctgacga cccacgccat gatcgtgggc atgaccggaa gcggcaaaac gggcctcgcc
     3121 atctcgctgc tcgaagaggc ggcgatcgac gggattcccg cgatcctgat cgatccaaag
     3181 ggagatctcg cgaatctgat gttgcagttc ccggacctca cgcctgatgc ctttcagccc
     3241 tgggtcgatc cctccgaagc cacacgcaaa ggagtgagcc tcgcagatct cgcggcggcc
     3301 acggctcaaa cctggcgcaa cggactcgtc gagtggggtc aggacccctc ccgcatccgc
     3361 cgattgcggg acggcgcgga gttcgttatt tacactcccg gtcacgcttc cgtgcgtccg
     3421 ctccagatcc tgcgttcgtt cgccgcgccc cccccggcac tccggagcga cgatgcggcg
     3481 ttgcacgacc gggttttgac ggccgtgtcc ggtctgctcg gcttgctcgg cctcgatgcg
     3541 gatcctgttc agagccgcga cgctatcctg ctctccaatc tgttttgtca cgcttggcgg
     3601 gaaggccgtg atctggatct tgccgccctg atccgtgacg tgcagaaacc gcccttcgcc
     3661 cgtgtcggcg tgttggacct cgaaaccttc tatccctccg ccgaacgcat ggggttggtt
     3721 atgcgcctca acaatgttct ggcatcgccc ggcttcagcg cctggatgga gggtgaaccc
     3781 cttgacgtgc aacgcctgtt gttttccccg accggcaaac cgcgccatgc gatcctttat
     3841 ctggcgcatc tctccgatac cgaacggatg ttcttcgtga ccctgttact caacgaggtc
     3901 accgcatgga tgcgggccca gtccggcacc ggcagcctcc gggccctgct ctacatggat
     3961 gagatcttcg ggtactttcc gcccaccgcc aacccgccct cgaaaacacc gatgctgacc
     4021 ctgctcaaac aggcccgggc ctacggactc ggcgtcgtgc tcgccaccca gaaccccgta
     4081 gatctggact acaaaggcct ttcgaactgc ggcacctggt tcattggaag gcttcagacc
     4141 gaacgcgaca agaaacgggt gatcgaaggc ttggagggcg cggccgcaag cagcggcgcc
     4201 ggcttcgacc gcgcacggac cgaacagctt ctggccggtc tcggcaaccg cctgttcctg
     4261 atgcgcaatg ttcacgagga ggaaccggtg ctgttccaaa cccggtgggc actgagttac
     4321 ctgcgcggtc cgctgaccct gccgcaactc gcgaccctcg cgccgccgcg ggacacgatc
     4381 cccgaggcgc aacccgtaac gcagacggcg gcgctttccg gcgcgtcttt tccgactgcc
     4441 gccacacccc tacccccagc cgcagcccat cccctacccc cggacctgcc cgtcttccac
     4501 cttcgccccg gcgcgtcatt ttccgccgac ctggtttacc gaccgatggc ggcaggcctt
     4561 gtcaacatgc gctttgtcga tgcgaaagcg gggctcgact tctggatgaa cgggatcgcg
     4621 ctcgcgccgt ttctggatga gggtgcccgc gccgcctggg aggagtgcga aatccggcgc
     4681 ggcacctcct ctgcccttct ggatcctcaa cccttgccga cggtggcgtt cgaaacaccg
     4741 cccgttcacc tgccggggtc caaacaactc gcgtcctggg ccaaggatct ggaaagtcat
     4801 ctctaccaga cccacacgtt cgagctgctc acctacccgg atctgaagct cgccgcgatg
     4861 ccggatgagt cggaagggga cttccgtgcg cgcatcatcc tgcggttgcg tgaacagcgt
     4921 gatgccgaac tcgcaaagtg gaaagccgcg tacgcgccga agttccagac cctcacggac
     4981 cagatccggc gtgcggaaga gcgcgtggcg cgcgaaaaag aacaggcggg ccagcagaaa
     5041 ttcaataccg cgcttgcgtt cggcgccaca ctgctgggcg cgttcctcgg ccgcgggggc
     5101 gccccggtcg gaacagtcgg acgtgccacg accgccatgc ggagcgcggg acggatccgc
     5161 aaagagagcg gggacgtcgc gcgcgcctcc gaaaatctcg aaacccttcg gcaacggttg
     5221 agcgacatgc aggaacagtt cgaccgggaa ctctccgccc tgcaggcccc ggtcgcggcc
     5281 gaagcgctgt cgctcgtcaa gcgcccggtg cggccgcgga agtccgatct ctccgtcggc
     5341 accgtgggac tctgctgggt gccgtggcgc aaacggcccg acggcatgtt cgagcctgcg
     5401 ggatgaccat gacaaaacag cgtatatccc caatgtccgc cacccccctg ccccctttaa
     5461 ccccaggtta acttatgtcc tgctcaatcc cggacacgcg aacccgactg ctcctcctgc
     5521 gcggtgtgct gcgccgcttt tttctaaacc tcttccgccc cgcctatgtg cgcgacagcc
     5581 ttgcgcgccg acaaggggcg tgcaaccgtt gcggtgcatg ctgccacctc gtggccaacc
     5641 gttgcgcacg gttgcgcgtt tacccggacg gccactccga atgttggcgg tacgaagggt
     5701 tccgcatgcc gaactgcaaa acgttcccga tcgacgcgcg cgatctggcc gagcgcgact
     5761 ggatcgcgcc cgacacgcct tgcggttatt tctggccgga tgcgcccgtc actccgcagc
     5821 cttcccgcag atcatgatca tcggcttgtc tgcgttacat caagtcctgc atggtcacga
     5881 ccggcacatc cttcttgggc gccgacttca gcggacgcag ccgtttggcg ttgaaacacc
     5941 accccttctc cgcgtccagc ggccaaaccg caggcgccaa cgccatcacc gtggcggcaa
     6001 acccctgaag cgtggccgcg gcattgcctg tgtgcgacga attgtcggcc tcgttctcga
     6061 tcacgaacgg ccggtcaaac ccgagctcca tcagggtctt caaaaacgcg aaccagtcga
     6121 tgctgtcgga accgccgaag cccggcagtt tcggatcgta gtggtgccgg tcccactcgg
     6181 acgcgggctg acacacccct gcgcgtttgg ccaacttcgc gtccacctgc tgcatcggat
     6241 agaggcgtcc ccattggatc gccgcgagac tgttcgtgcg gttacgggtc cccttgatgt
     6301 gaacgcggcg tagccggttc agatccgtgt tgcggaccac atcaaccgga tcgatatgct
     6361 gccatgcgtc atgcgagggg tcatacgtct ccccgtgcgc cggactcgga atgagcgcgt
     6421 acatcagctt gcgcgccgct aacgtgcagg gcaggttgtt gtacgtggtg ggagtcgaag
     6481 ccgggcgcca tccctccatc ggacagttct catacgtgag cgtcaccccc aagtcttccg
     6541 catagcggac gatcggccga aaaacgcggg cgtagcattc gaggttcttc gcaaacccgc
     6601 catcctcctt gccgagttca tggttatagc ccacgaacgt tccgacgcac acgtcattgg
     6661 cgtcaccgcc caacagatgc gcgatgcgga tcagcttgag gatgtggtta cggttggctt
     6721 cgcacaccgc cggatcgccg ccgagcagat tgtcgtacgc gccgaccgaa aaacgcaggc
     6781 cctctttgtt cgctgtttcc agcacccgcc gggcgtcctc cagcgtaaag gtctcgtaat
     6841 tcaggtgcgt ggccacatga tcctgccgcg acccgtcgcg ccggaacacg cacaactcaa
     6901 gcccctgagc acccacagcc ttggcgcgct tcaccacctg cgcaaaggtg agtttcggaa
     6961 aagccgaggt cattagccaa attggattgt tcatccctgt atccccttat tcgattttgc
     7021 cacgcggcca cgccgcatga cgttgtgatg cgcggaccaa cgcaaaccgc actggcccgc
     7081 gcgtgaaatc ggatcgcatc cctgccgcgc tcaacggagc aacatgaagg tgcccgcaaa
     7141 acgcagctcg acgtcgaccg taccttgcgc ggtgtccaca atcaccgtgc ggagaatgtt
     7201 cttgccggtg attccgtcac ccgttacttg ccaccgcgag aggcgcgccg catccgccgc
     7261 gtcgatcgcg ccgaaggtca tcacggtacg gcgccaaggc gtggacaacg gatccagttc
     7321 cgtgcgtccc agatcgatgc tgccggcctg ccgcacctcc agcgtttcgg aaacccgtac
     7381 cagatcgttg gtcgtatcgg taaccgtgca ggcatagatt aggttcgtgc tgtcgaggag
     7441 gagatggcgc accgtgagcg tcgctgtttc catctcccct tcaggctcca gccgctcgct
     7501 cactgccagc accccgttcg atatcgtgcc cccacccgcc acggacggcg tgtcgttggt
     7561 cgagcccaac agatcgtaca cggcggactc gcctacccgg accgccggac gcggcgtgac
     7621 ggcctccgcg cacagcgtcg cgatctccgc ggccgtcaga gccctgctga agaccgccac
     7681 atcatcgatc atcccgccat cccgcatcat ctcccggttc ggattatcgg acgtggcgat
     7741 cccgcgtccc aaggtcaaca aaaatccggg cgtcagaagg gcggaggtga ggccgttggt
     7801 gtacgccttc cactccccgt ccagataagc gtccacacgt ccgggaacca ccacataggt
     7861 gaccatatgc cactcgccta tcgcgacgcg gccccccatc tccggcttga tgtgcgcacc
     7921 atgcccgctg ttcccgggaa tcaggttcga gatatagtcg ccgtcattgt atttgaagtc
     7981 gaacgtcccc cctgtactgt tttcagcggc attcacccgt ccgctgaaga ttccctgatg
     8041 cccccctttg gatgccagtt tgaaccatgc ggaaacggta aaggaggtga tgtcgaaaac
     8101 cgaggtgtac gccattttca gagaactcaa gccggtgaac cgggccgctt tgccaagcgc
     8161 tccgccctcg acgaaactca ccagattggt gtcgttctgc tgaagcaggt ggaacccgtt
     8221 accggattgg tcgttagagg tcccgtcgtc aaacgtccag agcgccagca acccttcagg
     8281 atacaggcgt tgcggccgtt gatttagccg cgcccgcaag gttcccgcct gaaccaccgt
     8341 ctcgccgcac cagacgttat tgctgtgcgc gagtgtcaac gccgctgtac cgcgcttgat
     8401 aagtccaccc atcggttcgg cggcgcgtaa cggctgttgc agcgcgacat ccgcatgcgg
     8461 cgtctcaatg atcgcgccgc cggaacggag cgtcagcgtg tccacaccga acaggtaatc
     8521 agagagcgaa gagggttcgt atggcgcgac tccgatcgtc ttgaggaccc cgccgttaaa
     8581 ggtgaaagcc gtgcgtcctc cttgccccga ggcgtacggc acagcccgtg tggtggccac
     8641 cagattcaac tccccggatt cgggtgtgcc gttgcccagc accaccgtat tgtagcggga
     8701 tcggtaggca tcggacatcc acgagcactg gcccgcaatt gtcagacgtc cgttgttgat
     8761 ggccagatgc cctcccggcc cattccaaac gtggctcgaa aagtcgttga cccccatcgc
     8821 gaaacggccg agcgtcatcg ttccgccatt gaccgtcaga taccccggac ccttggatcc
     8881 gacactcgtc catgaggaga ccggagcggt gaagattccc ccgttcatct ggtatatgcc
     8941 gtccgaataa ccgtaatcac gaataaacaa cccttggccg tccgtgacct cgacaatccc
     9001 gccctcctga cgcaccagcg caacacactg ggtcgcgtct gcaagagtgg tgttcagatg
     9061 aatcccgcct gtccgcaggc gcgcgccctc ttcgatcacc aactcggttg ccgcgttcgc
     9121 gtcctgtccc agtttaagcg tgtcgcgcgc gccgctgaac agcgtgtagt ctgcccctgc
     9181 ggcaaaccgc aatgtgccgc gcaacaagtg cagttttcgt cccgactcac tcaatgtggc
     9241 ctgccccgcc aacgtcagcc cgttgggaac gtcgaggcca agcgtggcca ccgcagggaa
     9301 cgcgaccggg ccgtcgagcg tcagtccggc cgagtccgca gcaagttggg aggaggcgct
     9361 gacgaccaag ggggttccta acgtcgtgtt ttcactcccg gcatagcgcg ccgttccacc
     9421 cgcgagttcc aaaggcatac tgccgaacac atcgttggtg aacaggagcg tacccgactc
     9481 gaccgacacc ccgccgatat tgccgagcgg tgccgccaag cttagcgcgc ccgacccgtt
     9541 ctgcctgagc ttgccgcccc cctgcgactc ggacgcgagg gtcagcgtca cattcgttgc
     9601 gggccgcacc ccggctccac gcaatcccac ctccagcggg gcgtccaaaa ccgtgctctg
     9661 cgtgatctcc agtatgccgt tatcaaacgt gactccaccc gccaacgcgg caccgtccgc
     9721 gatggacagg aaaccgttcc ggagcagggt caaagtctga ttcgtcccca gaagcgtgac
     9781 cccgcccgcg ccggtcaccg tcatcggccc gatgccggac accgcatctg aaagggtcag
     9841 cgacgcgcct gcagccgttt ccacacgcat ttcatctgcc agcacccagg gcacagccat
     9901 ctcatgctga ccggattgga gcgaaaggaa cgagggttgg gaaccggaat caaaggtcag
     9961 cacgttcgtc ccgctgaacg tgtacgggac gacgttattg ctgaggacca gattgccgac
    10021 cgtgaccggc gcatcgagcg tcaccgtcac cggagcggtc gcattcgtca gaaaggccgc
    10081 aaacgctccg gcggcatttg gaaccggacc gtcccagttt ccgggaacgg accaatcacc
    10141 gtccgcatcg gcgatccagc gtgcaccgct gcgctctgaa atcgtcacgg agatcacccc
    10201 gtcactctcc gtgaacgcat actgtttcgt gggatcggga ttggccacgc gcagatcgga
    10261 tgccgaaccg gtcagtgcgc cgctgaaggt gatcaacgcg tacgtgccgt tggcggtgaa
    10321 ggccgtggtc gtgccgggct gatacaggaa cacgtccccc ttgccggaaa gatcgagcgg
    10381 cccggtcaca tccagccgat ccgtgttctc ctcgtccgtc acctcaaaga cgagttgacc
    10441 cgcgccctgc gcgtctccga gcgtcattcc cgtaagcgag aggctgtccg tcatcccagg
    10501 ggtgaagaga aagcgcgcgt tccctcccac tgtcaggctt ccggcaaaga agccattcga
    10561 aacgacggtc gccgtggtga acggcgcgag ccggagcgat tccgaggcca cggccgcctg
    10621 gatcgtcagg gtgccgttcg aaacgactgt cgggccggta taggtgcagt ccgcggccag
    10681 cgtcagggtt ccgccgccca gctggatcag tccgccgtcc ggcgtcgcgc cgctctccag
    10741 cgcgtcattg agggtcaccg caaacccctg cgtgtccaaa accagccccc cggcctgaat
    10801 acgcgagtcg agcaaatccg gccgggcgcc gaaaaacccg gcggtcgttt tcccggcgcg
    10861 cacggtgccg ccgttaaagg cgagtgtggt gagcagattg gttttcgcgc tgtgcttaaa
    10921 cccgccggtc aggaaaagtc cggcgttgaa gtcgatccgg ttggtgatcg tcccatctct
    10981 cttcggattc gagagcctgt accccacatc gaactccccg gccccgtcgg ccgagaattt
    11041 gaacatcccc ccattcagga taacgtggct tccgccgttc tccgccaatt cctgtccggt
    11101 cgcaagccac tcgaacaagc cgtcaccgct tagcgtgagc aacgtgttac cgttgtttga
    11161 ccagcgcggc gcccctcccg cagtgaacac gccgccccgg acattgacca cgcaggtctt
    11221 cgtccggtcc tgcgccgtga gcagcgagtt ccccacgacg atccggccgc tgtagagatc
    11281 cacggtgcat gcttgccaga acacatagga cgccgtgaaa ccggaaagcg tcgcgttgtt
    11341 cgtgatgatg agcgacgcat tgttctgcag atccaaccga ttgaacaccg catcgctggc
    11401 cacaatcagg cttccgccgt tgatcttgct gaccgggtcg accgccgtga aattcgcgcc
    11461 gccctcgagg atcagccgtc cggagccatt tttcgtgaac gcggcgttcc cggcaccgct
    11521 ggtcagatgt ccggcgagaa ccacatcccc cgcaccggtg tcgagcgtgc ggctcacccc
    11581 ggaccaggcc acatccgccg caatacgctg cctccccgtt gagttgttgg cgagattgcc
    11641 gcacagaatg aacgggttgc cggagaggtt gaacggcgca gaggacgccg caaaagtcag
    11701 gttggaaaag aaagaccccg gctggaaatc gttgaccgtc tgggtcacgc ttccgtcgaa
    11761 cgtgaggctg gtcagcggac cggggaccgg tgccgcccca ccctgccaat tgccgcctat
    11821 ggaccactgc ggcccggcac cgcctccggt ccacacactt tgtgagaatc cgcgacacgt
    11881 catcagaacc agaatcggca ccagaagaac ccacaacctg cttctcattg cgatcccttt
    11941 ctcctttatt tatccgccat attcaagtga gtgtcacaag acggcaacat gatatggtgg
    12001 gaggataccg tttccgccga ctcccgtcaa tcccaagcaa aatcggaaaa cgttttgggg
    12061 agtttccgat gtttgatcgc caatcgctgt gcgcgggaag cggcctcagt ggtctgacgg
    12121 aaccgtttgc tgccggacct gcggaaccct ttggcgagtt gcagacgcca acgccgacgg
    12181 gcactgactc tggagatggg ttacgcccca tccgattaat tcctttgcgg cgtacgcccg
    12241 gcgtttttct tgaaaatagt ggaactcggc tttttccgca tctgtccagg tattgtgctg
    12301 cgtgtatgaa ccgcagaaag gcacaaggag gtcaagccag caggcgaaga cgcgtttttc
    12361 gacgtcatcg accttcacgt cgtgatgccc cggctcaaag tgcgccatga tcttgctctt
    12421 gcaggcaccg gtatgatacg gcggcagcat cacggcacgg ctgcgcggct tcaaccatgt
    12481 catccacggg ttacggtcgg ggttgcccga tgtggtgatc gcaacatagg agtgggtgta
    12541 cgcccttttc ggatccacct gaccggcacc gatcagcttg atggtttccg gcttcgccgg
    12601 ctcgccggtg agactgatct tgcactttgc gagattgtcg gcattgtggc aacggataca
    12661 gtgcttgtcg aaaataggct gaatctcctg cacgtagctg aatccgtcca cgggagcgtc
    12721 tgcgtcgagt gagcgcggcg cgttgacccc cagataattc tccacgctgt cgcgccacga
    12781 ctgtgttttc agccgtttga tgagcgggtg ctcccgaccg ccaaaagact tcagtttttg
    12841 cggaggcttg gcaagggcca gcaccgtttt cgcagaactc gccgcatgcg cccccgcatc
    12901 atgtttgctt tcatgacaac cgatgcaggc gaaatgctcg ccgttttgaa gcgtggacca
    12961 acttctcatg gactggatgg cgtagccgtg cgcgtcgaga acctgaaaat agaccggggc
    13021 gcgcgcgggg acctcgaagc tgcaactgcc gtcagcctcg atatcgacct cgccgaggac
    13081 atgcttgacg tcccaactgc cgctgttcag cgagatcggc gtttggcaaa gtccggtttc
    13141 gcactcaccc ccgttctgtc cgtaccccat gcggcagacg cggtattcga gggcgacaac
    13201 gcgaattttt ttcgccgtgc cgcgcggtat gccttgcagg cccggcccct ggtagacatc
    13261 ctgcacggta tagatgccgt gattgtgcgt cggcatgata tgggacgggc gcacgggggg
    13321 cggtgtccgg cgcatgacag gaacagcccg tcccgtgctc tgccaatcgt catacgcgag
    13381 cagttcgcgc gcgccgtcgg cgtgcatgta gtaaagaccg agcggtggat tgaacgggcc
    13441 aaaagtcggc aggtatcctt ccgggcagaa cgagcagaaa tagttttcct catcaaaggc
    13501 aaagggatgc gcgtattgcg gcccgaactg accgaagacg tcgatcttga agtcatggta
    13561 gggacgggca gggtcgggcg gctctggccg gatgaagtca gccgactcgc ggcccagccc
    13621 gccattgagc cgggtgttga caaattcgat gccttgtccc gcctgggttc cttcggtacg
    13681 gtcgatcaaa ccgagcttgc ccttgtagat ggagtgatgc cccgcaatca cagcgatcgc
    13741 cttatgcgag ccggggatac cgcgggcatg aatgattgcg gccgggaaca tgctgttgtt
    13801 cccgtagtat tccgtctgcg atgtcccgtc gggattcatt gcgatcaggg ggtgcagata
    13861 gagcgcgctg cggtcgttat actcccagcg ggtaaatacg atgcggccgt cttccagcac
    13921 cccggggttg ctggtcatga gctggtcgga tgtgagacgg cggatgccgc tgccgtcaat
    13981 gttacaggtg taaatgttgc cgcccgcgcg gtaccagcaa tcgctgatgt gtacgtcgcg
    14041 cgtggaagtg aacgcgatac gtccatccgg caggaacacg ggctcgcagt cggcaacgtg
    14101 cagcgtcttg ccatccttga cgatgggaaa cgtgatctgc cgcaccttgc gcgacgccac
    14161 atccatcgtg tagagatagt atgagtcggt atcaaagtga tcgcgcatcg cgaacaccac
    14221 ggtctgcgcg tcccaagaga ggctgggata acagatcgcg ccttcgggtt tttcgatcag
    14281 cacctcatgt ttgactgcgc cgtcctcaag caaggttccc atgcagagtt gtccgccccg
    14341 cttgaaaccg ggcgtgaaat cgcggacctg ctcgtcggga atgtcatatt tgcccaccag
    14401 tccctgcatg cagccgaaaa tgccgtgttt gacgtagatg aaccgatcgg ctttggcacg
    14461 gatgtctcgc aggcgcactt tgcgtctcgc ggcacaggca tcgagataca gatcgcgcca
    14521 gcgccggtca cgtgcgggca ctctacccgt atcaagagac tccagccgtt gccgcaacgt
    14581 ctttgcggca gggtcatcca caggcacgca ccccactaca cgctgcacca tcgcgcgttc
    14641 cttggcggca tcgttcggcg aggtgaaaac ctgggcgatg tcgagcccgg catcttgata
    14701 gagccaatcg cgctcaagcc ggtggcgcgg aaaggcggtg tccgccgtgt ttttgaccgg
    14761 aacatctgca ggacggcggt cataacaggt gatctccgcg atctggacat tgagattttg
    14821 accgtcaccc gcaatcggca ggcccacctt gcgggaccag gggatttgcg cttggtgcat
    14881 ccatagtgcc tttgggtaac gtttttgata tgaatcatgc acactcaccc gcagataacg
    14941 ggtcgaaacg ggcttccact ccacaaaggc agaatttgag ttggagaacg cgggcaattc
    15001 gcgttttcct gccaggggcc ggacctgctc ctggcctttc gcgtcggcac aggcagcgac
    15061 actcacattc ttcggcccca catagaccca tgcgttacgc gccacgaacc gcatgccgca
    15121 caccgtcctg ctcgcaccca gatccaacac gaattcggct gtcaccggcg gctcggcctt
    15181 gggcggatag acacgcgtgt cactgcccgt gcgcgtcgtg tccagcagac aggcataggt
    15241 gcccaaatcg ttatcgatca tcttgtctac cgcatatttt ccgggcggat caggatagca
    15301 ttccgcgtct gtcgtcacag cgacaggacg gaactcttcc gcaacaagca ccccgctgca
    15361 taccgcgagc gagaacacga gtagaaagtt ttttttcata tccatgacac gctccacatc
    15421 cgaaactgtg taaccatgcc gttacgcacg cctcatcccc gccttttact ttccagtatg
    15481 aaaaaaggca caccctttgg caagaatttg attgtatgat ctattcaaaa aaagtgcgat
    15541 tttgacatga tgacccgata aaaaaggggg gccgtccgat ttggcccccc acaataaatc
    15601 acacactgcc caccggtttc acaccagtgc ggcacgcgcc ttttcgatca acaccttgaa
    15661 cccctccgca tccgcgatcg cgatctcgga aagcattttg cggttcaccg tgatcccggc
    15721 cttggtcagc ccctcgatga agcggctgta cgtcatcccc tcattgcggg ttgccgcatt
    15781 gatccgggct atccacagcc tgcggaaatc gcgcttgcgg agtttccggt gctcggtcgc
    15841 caaccgctgt gcgcggtcaa cggcctcggt ggcctgacgg aacagtttgc tacgggaccc
    15901 gcggaaccct ttggcgagtt gcagacgccg acgccgacgg gcgcgggata cgggcgcatt
    15961 cgtgactctg gacatgattt acgctccttc cgaattaatt gccttgcagc atgcgcacgg
    16021 cggttttgac gaacacgtct ttggcgacag tggttccacg gagattgcgt ttgcgcccgc
    16081 gcgacttata gccgttcaag tgggacttgc cactctggga gcgaatcact ttacccgtgg
    16141 ccgacatttt aatccgcttg gtagcggatt tcttggtctt catggtaggc atattacttc
    16201 ctcgcttttc ctgttgtgaa gcctgaaccc gtagggcagt cagccgtgaa agcccgtaca
    16261 ggatcgtacc cttttcccct tagcggagaa aaagatttaa cgttatttaa tatcaaaacc
    16321 gtccctggcc gacaagccga aaggctacag gactttttcc accgcacttg tcaaaacaag
    16381 ttcgattggg ggggctgcat tcactttgcc aagcgcggcg acataaaatc gccccccaga
    16441 tcacgccgcg ccttgcctaa ttcctatttg tatgccagaa taggaacagt tgctcacatg
    16501 aaaagaatgc gtgctatctg tcttgtatcc agtggcgtac tggttactgg ctggtggaag
    16561 aaaggaaaca cacaatggca agaataacga ttccggttga agagtttaaa cagcgcgttg
    16621 cgaatgcggc gaaactcgtg caggctaagg gcctcgacgt tctggtcgtc aacggctctg
    16681 aggcagatta cgcggacaca cgctatttct cgaatttctg gcctgtattc gagcgcgtcg
    16741 gcgtcgcgat tgcagcaaac ggcgaagcgg cgctcatggt cggccccgaa tcacaggtct
    16801 ttgctgacga tttcagttgg atggaccgca tctgcgtcgt ctatcagtac cgcgagagcg
    16861 cgaaccccgc ataccctgag c
//
LOCUS       DAOSLE010000058        16303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3735_length_16303_cov_9.723474, whole genome shotgun sequence.
ACCESSION   DAOSLE010000058 DAOSLE010000000
VERSION     DAOSLE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3735_length_16303_cov_9.723474"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="PK576_05620"
     CDS             complement(<1..1581)
                     /locus_tag="PK576_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE61107.1"
                     /translation="MGGRLSIMIASLAVCSAVGDTLPGTFLFDLGEATVTTLAERQQS
                     LSKETLVGATDDMLQRYAPAGEVPNAINAFLVHTGGKRVLVDTGLGLKLEENLKSAGV
                     SPVEIDAVLITHMHRDHIGGLLRGGAPVFTNATLYISHKEFDYWKAAGAEAQRIADAY
                     AGRLRLFHPQDLDAGQPVPLQRNIVAFAAYGHTPGHTIYLVEGTKKKLLIWGDLTHAA
                     PIQVPHPEVAVTYDVDPAQAVATRQRVLEFAITNKILCVGSMHLAYPGIGHLYRRQDT
                     NTCHLIPVDYTYTNAVSGREIFVRSDGYRTETERIRLGAAKGKVRYLRESHQGSQWWQ
                     ITENTYDKRGKPVRSVKELYYKIPEKPEVEETVYFPNRYHAHYVKKTGRDGVTRYSVF
                     NPKGEFVATVSGDGKLIKEEKPGNDRYWAQFLQGETPDEARARLLKAEQARQAAERAR
                     QDAEKARQAAEDEKDAAAEKVIAASATIMRFKLPAEIESLFPSNLYSSLPPGTLDWVA
                     SMRKAHFESERALMLLCKS"
     gene            2203..3195
                     /locus_tag="PK576_05625"
     CDS             2203..3195
                     /locus_tag="PK576_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOE61108.1"
                     /translation="MGNGERGTQDEKNFGVLSSAFGVLSSCRLCPRACGVDRLAGQRG
                     YCRAGVLPHVFRYGAHFGEEPPITGTRGSGTIFFSHCTMRCIYCQNHPWSQAGHGDDF
                     TVEALRDILEQLARQGCHNWNLVSPTPWLPQIREAAEPLLRTGIALPFVYNTSGYESL
                     ETLEAFADLADIALIDLRYARAESAAEGSDARTYVEAARGALRWFWERLGPLETDASG
                     IAVRGVICRVLALPGRIAEAVENLEWLARHTGSGIHVSVMSQYTPLHRALQTPGWDRK
                     VTADEYAVLVEAAEDLGLGNGWIQEFEGEAPADLLGQNMPAGEGAVGAGSNTPV"
     gene            3250..4017
                     /locus_tag="PK576_05630"
     CDS             3250..4017
                     /locus_tag="PK576_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE61109.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGKIFSKIAKELTVVAKAGGGDPN
                     TNPTLRTLITKAKAVNMPNDNIDRAIKKGTGELAADVLEEITYEGYAQGGVGLVVKVL
                     TDNKNRAAAEVRNIFKKNGSDFATLGSVSRGFERKGTIMIPAAEGLTEDKVMEVVLIA
                     GADDMESEEDGFTVTCSPAAFSEVCAALEAAEIAFDQENSLVGLVAQTLVPVKELAVA
                     KAVNKFVDALEENDDVQDVFTNMDIDDALVEQLEAEA"
     gene            complement(4145..6229)
                     /gene="fusA"
                     /locus_tag="PK576_05635"
     CDS             complement(4145..6229)
                     /gene="fusA"
                     /locus_tag="PK576_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE61110.1"
                     /translation="MSRTIPLDQIRNIGIMAHIDAGKTTTSERILFYSGKNYKLGEVH
                     EGAATMDYMVQEQERGITITSAATAVSWRDHQISLIDTPGHVDFTAEVERSLRVLDGA
                     IALFCAVGGVQPQTEQVWRQSEKYEVPKLCMVNKMDRIGANFFNVVNEIRYTLGGNPV
                     VMVYPMGVADSFRGVVDLLTMKALVFDEEDRGVRVLVEEVPEEFREECERLRVEMMEQ
                     AAEQDDALMAKYFETGELSIEEIKSGLRKGTINRKLAPTYCGTAFKNKGVQPLLDGVI
                     DYLPSPADIGSVRSADNPENLRSPDENEPFAGLAFKILSDKHMGKITFVRLYSGTLRV
                     GDEVLNSTMEQPGRIGRLFRMHAARQEALEAAYAGDIVAVVGLSKTRTGDTICDPKHP
                     ILLESIEFPAPVISIAIRPASRGDNEKLGNALYSLAQEDPTFVVRYDQETGETIISGM
                     GELHLEVIVDRLRREYGVACEVGKPEVAYRETIRHMVDGEYKHVKQSGGRGQYAHVCL
                     RLEPQEPGRGFSFVNEVKGGVIPNEYIPAVEKGVIKAMENGPCGGFPVVDVKVVVHFG
                     SYHAVDSSEFAFVECARQCFKNLFLKGNPQLLEPIMSVEVAVPEDYMGAATGSICQRR
                     GRVEGMDEKAGSKLIRGLVPLGEMFGYSNSIRTLTQGRGTFSMTFERYEGVPFELAET
                     IIEKRKKEGKVR"
     gene            6463..7836
                     /locus_tag="PK576_05640"
     CDS             6463..7836
                     /locus_tag="PK576_05640"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOE61111.1"
                     /translation="MIRPSLPVEYFERNRETILKEWFALLRLPTVGTDPKRLGECARA
                     AAWLKVFLRKLDFSVDIITPESGHPVPVVLAERPGNGTSTVLFYGHYDVQPEDPLEAW
                     LTPPFEPTLINGRVYARGAQDNKGQVFGFLQGVKALIEAGVPLPTLRIVIEGQEESGS
                     EGLTALAPSLRQRLQADVMMVCDTSCGPENQPAIIAGLRGVQHFTLTLNGPAYDLHSG
                     IHGGVAPNPAQGMARLLASLHTPDGAIAVAGFRDRVRPPEAEELEHARRAAAPAENYE
                     AEIGCPPVGGDRRLDPVARGSFEPTIEINGIHGGYGGPGSKTVIPSTVLAKLSMRLVP
                     DQRPDEVFDAVRSHLEAHCPRGLKLSLTEICPGAPGFRLELHSPLFRLAADVLERLDN
                     RGALFRWEGASIPVVALLRQVSGAAPLLVGFGREEDKIHCPNESFGLDQFERVMTWSA
                     MMLSELA"
     gene            8010..8396
                     /locus_tag="PK576_05645"
     CDS             8010..8396
                     /locus_tag="PK576_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE61112.1"
                     /translation="MTTFDVQTSLHSQVVNITDSIREAIKSLGITDGVVTAFVPHTTC
                     GLTVTEGDDPTVTADLLRQMDELVPWDRPYYTHTGGNAAAHIKSALIGTSLHLIVKGG
                     TLQLGMWQGIFLCEFDGPRIRHVWVR"
     gene            complement(8398..9930)
                     /locus_tag="PK576_05650"
     CDS             complement(8398..9930)
                     /locus_tag="PK576_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOE61113.1"
                     /translation="MMKANRKITMSGTFIGVLGLSGWVACGAELDGVMSEAYWKLWNP
                     EVQVRIDRDIEKYRKADATLVLEGVAPGVDVKVEQLSHDFIFGAHIFNFNQLGSFERN
                     RKYKELYGTLFNSATIAFYWKKFEMEPGKLRFAEEERDTEAYWNSVKEPKKEPHWRRP
                     ATDPVVAFCESKGIRLHGHPITWGNKTWHYPEWLMERNCPAEEKAEIQKLGKDGLQKL
                     SPAEIAALLPVYTREINRRFTTRVEELARHYQGRIHSWDVVNESATDFSKGMMIPGDV
                     ICKSRYGLMPGDYTFASFQVAQREFPGTVKLNINDYLNTEAYVRQVKDLMARGCRIDI
                     MGSQMHLFNPRQCLDIAAGKPIESPDQVWSKMALLSQAKLPIHLSEITITSPGGDEKG
                     CKIQAVIARNLYRLWFSIERMMGITWWNVVDDCGAPGEPSVSGLFTRDMQPKPAFHAL
                     DKLIQSEWKTKLTCKTGPDGRVAFRGFKGRYRVSWRDAAGQTHSRGFHLSKDGDGILD
                     PL"
     gene            complement(10065..10940)
                     /locus_tag="PK576_05655"
     CDS             complement(10065..10940)
                     /locus_tag="PK576_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE61114.1"
                     /translation="MKNVIVLSACICFSCVGPHCVWGSDLVRPGETQESAKRGQSKGE
                     PTNAKEAADKKSATAAAIDAKYAEIVAKLPPEEQAWERTLQANLGGFYLPRYKEGRVR
                     GREQAWDYVKDVPGLPRVLLIGDSVSRGYTLATRKALVGKANVHRAPENCGPSANGLK
                     KLDIWLAGEKWDVIHFNFGIHDRATPPDVYRQRLETLVTRMKATGAKVIWASTTPIPP
                     DTQDGPKASEAIVEKNRIAAELMTRHGVAINDLFTFITPHLSKVQNPKDVHFKGEGYD
                     LLGKRVAEAITAALK"
     gene            complement(11127..13832)
                     /locus_tag="PK576_05660"
     CDS             complement(11127..13832)
                     /locus_tag="PK576_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE61115.1"
                     /translation="MLAVVAGCAAAGAIPHRVFVLENGLRLVVHEDRKAPIVAVQVAY
                     HVGSKNERPGRTGFAHLFEHLMFNGSEHFDDDYFKALERVGATGINGTTNEDRTHYFQ
                     TVPREALDTALWMESDRMGHFLGALTQERLEEQRGVVLNEKRQGDNQPYRIAWRLITR
                     HAWPLGHPYSWEVIGSEVDLAAASLQDVRAWFRDFYGAANAVLVVAGDVSAEEALQKV
                     RHAFGDIPPGPPVARPVVDVVRRTGARRMRVQDRVPQARLYKVWTIPQYGTTEGNRLT
                     LAAEALAAGKSSRLYKRLVYDTQLATEVHAWADLREIAGQFGVVITVRPGVSLAEAER
                     AADEEIARFLREGPTRRELERIQARIEADFIRQSEQVGGKADMLIRDLLFTGDAAYHE
                     KRMREWREATPRQVVETAREWLSDGAFILEVEPFPAYRVATAGADRSALPLPSELPEA
                     AFPAVRRATLSNGLRVVLVERHETPLVLCTLSVGAGFAADACAQRGTATLMADLLDEG
                     TRHLSALELGRELERLGAALSTGCAPDATLVSLNTLSRHLDRALDLYAEVILHPAFPE
                     EDFQRLRHQRLAQIRREKANPRGVAMRVMPRLLYGERHAYGQGFSGSGEEESVAGLTR
                     DDLERFYRQEFKPNRATLVVAGDVTLEALLPRLERRFGSWKPGEVPDRSVAEARPALR
                     PSVWLIDRPAAPQTLIVAGGIAPPKRHPLDPVIEALNTLLGGAFTSRVNMNLREEKHW
                     SYGARTYLTEVCGPRAFLCIAPIQTDRVADAMRELDREFRGITGTNPPTAEELRKAVA
                     HHTLQLAGRWETLGAVVGALEQVVRFDLPEEYYSVYAGTMRALTLPEVTRAAAEVIRP
                     ESLVWLVVGDRTQVEEPLRRLDWGPVNVLDADGKPVNGTVGSVSR"
     gene            complement(13872..15203)
                     /gene="dnaX"
                     /locus_tag="PK576_05665"
     CDS             complement(13872..15203)
                     /gene="dnaX"
                     /locus_tag="PK576_05665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOE61116.1"
                     /translation="MAYEVLARKWRPQQFADVVGQEHVTRTLKNAIESGRVAHAYLFV
                     GPRGIGKTSLARIFAKALDCEKGPTATPCCECPLCREIAAGNALDVLEIDGASNNGVD
                     QVRDLRDQIQFAPTHGRFKIIIIDEVHMLSTAAFNALLKTLEEPPPHVKFIFATTEGD
                     KVLPTIISRCQRFDLRRIQTPLIVERLRHICNAERIRIDEDALLAIARGAEGGMRDAL
                     SALDQLISFKGGALTEEDVLAVFGLVSRASLEGLATAVLTGDMAEILRLVDQFDSAGK
                     DMRRLVVELMGHFRNLLVYQQVGEQMSTLDATPEQIRSLAAQAAHADSSRVLQIADQL
                     GEMEGRLRFALSMRTLIELSLIRCARIARAVSLEEVLKRLNALRGSDPTPAAAPSAAS
                     SVQSGAAAAGPLPGVAHDDPALKQVMQLFGGEVVDVDDGKGRSAQQKSSGA"
     gene            complement(15307..>16303)
                     /locus_tag="PK576_05670"
     CDS             complement(15307..>16303)
                     /locus_tag="PK576_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61117.1"
                     /translation="ITCTTITRGRRIDKPWRYVTCEAGSDRVVKWADADSKDIIFSNV
                     NYQALSTPNAATYKTNHINGLSTVYFDHSHIRYLAGGVPCSLRTYFGVVAHGHDATPF
                     GILDGVFGQGGVDAGIRIHNGTSYSDPIALTDRVNGDAGSAFAKGTPHLFSRVYPGNM
                     KWDSPALSDYSPHKRPMSGDICEIVVYGRVLGETERSLVEDYLMAKWGLKNLPDPAKY
                     ADVLPTGTDLTVASAATLDLNGNDQTLASLDLSGTLTNSVERVATLTLSGVSFVRAGA
                     SFGAPNLDLVLSDDAVLDLGGGTFTIRRLTGRRVQVINGVLNETKPLLDFLLIIR"
BASE COUNT         3135 a         5231 c         4703 g         3234 t
ORIGIN      
        1 actcttgcaa agcagcatca aggcgcgctc ggattcaaaa tgcgcctttc gcatgctggc
       61 gacccaatcg agtgtaccgg gcggaagcga agagtagaga tttgacggga acagcgactc
      121 gatttctgcc ggcagtttga atcgcatgat cgttgccgat gccgcgataa ctttttccgc
      181 cgccgcgtcc ttctcgtctt ctgcggcctg tcgtgctttc tccgcatcct gccgtgcccg
      241 ctccgcggcc tgccgcgctt gctctgcctt gagcaggcgc gcgcgtgcct cgtccggcgt
      301 ctcgccctgc aaaaactgcg cccaataccg gtcgttgccc ggcttctcct ccttgatcag
      361 tttgccgtcg ccggaaaccg tcgcgacgaa ttcccccttc ggattgaaaa ctgagtatcg
      421 cgtcacgccg tcacgccccg tctttttgac gtaatgggca tgatacctgt tcgggaaata
      481 gaccgtctct tcaacctccg gtttttccgg tatcttatag tagagttctt taacggaacg
      541 aaccggcttc cctcttttat cgtaggtgtt ttccgtgatc tgccaccatt ggcttccctg
      601 atgcgattcc ctgagatatc tgaccttgcc tttggcggcg cccagtcgga ttctctctgt
      661 ctcggtacgg taaccatcgg agcgcacaaa aatctcgcga ccgctcaccg cattcgtgta
      721 tgtgtaatca accggtatga ggtggcaggt gttggtgtcc tgccgtcgat acagatgacc
      781 gatgcccgga taagcgagat gcatgctgcc gacacacaaa attttatttg tgatggcgaa
      841 ctccaagacg cgctggcgcg tcgccacagc ctgcgctgga tcgacatcat aggtgaccgc
      901 aacttctgga tgcggcactt gtatgggagc ggcgtgtgta aggtcacccc agatcagcag
      961 tttctttttt gtgccctcca ccagataaat cgtgtgtccc ggcgtgtggc cgtaggctgc
     1021 aaacgcaacg atattccgct gcagcggcac gggttgcccg gcgtccagat cctgcgggtg
     1081 aaacagacgg aggcgtccgg catacgcatc cgcgatacgt tgcgcttccg cccccgccgc
     1141 tttccaataa tcaaactcct tatgggaaat atacagcgtg gcattcgtga aaacgggtgc
     1201 gccgccgcgc aacagacctc caatgtgatc gcggtgcatg tgcgtgatga gcaccgcgtc
     1261 gatctctacc ggggacacgc ccgccgactt caggttttcc tccagtttca gccccaaccc
     1321 tgtgtccacc agcacccttt tgccgcctgt gtggactaga aaagcgttga tcgcgttcgg
     1381 gacttcgcct gcgggcgcgt aacgttgcag catatcgtcg gtcgcgccga ccagcgtttc
     1441 tttgctgagc gactgctgcc gctccgcgag cgtcgtcacc gtcgcctcgc caaggtcgaa
     1501 cagaaaggtg ccgggcagcg tgtccccgac tgccgaacaa acagcaagcg acgcgatcat
     1561 gatagataaa cgcccgccca tattccctcc tttgtcgggt gcttgagaag ccgaaatatt
     1621 acaacacgtt cacctctgcc ttgcaatgct ggacgtgggc gtcctgcgtt cgggtaatcg
     1681 gttcgtgaca gggggcatgc gagttgagag cgtgttttgt gtatgcgcag tcgggagcgc
     1741 gggcgtcccc gcccgcaaca acgtgtcttg tgctttctgg ttttatccgc agatttacac
     1801 agatttcgca gattttttgt tctgcccgca ggacgggagg gacgagcgtc tgctcgtccg
     1861 ggcggcgttt cacgccgccg cctttgaacg ccgaacgccc aacgccgaac tcagaacgtc
     1921 gaagtgaaaa aactcctaac tcctaactcc taactcctaa atccacgcgt agcgcggaag
     1981 acgcccgcgc tcccgactct ttgcgctccg cgtctctgca ttaaacaaac ccgagcagtg
     2041 ttccccaaaa accttcgtgc ccttcttacc ttcgtgtgag aaaaaagagc agagccctga
     2101 aatccgtttt cacctttttt aggttgaatc gactttctct tgaaaacgcc gaaccttccg
     2161 tctacagttg accgcatgcg aacgcagaac gaaggatgga gaatggggaa tggggaacgg
     2221 ggaacgcagg acgaaaaaaa cttcggcgtt ctgagttcgg cgttcggcgt tctgagttcc
     2281 tgccgtctct gcccgcgtgc atgcggcgtg gatcgcctcg cgggccagcg cggctactgc
     2341 cgcgcggggg tgctgccgca cgtcttccgc tatggcgcac atttcggcga ggagccgccg
     2401 atcaccggga cgcgcggttc gggcaccatc ttcttctcgc actgcacgat gcgctgcatc
     2461 tactgccaga accatccgtg gagccaagcg ggacatggcg acgacttcac ggtagaggca
     2521 ctgcgcgata ttctcgaaca gcttgcgcgg cagggctgcc acaactggaa tctggtctcc
     2581 cccaccccgt ggctgccgca gatccgcgaa gcggcggagc cgctacttcg cactggaatc
     2641 gcccttccgt ttgtgtataa tacgtcgggt tacgagtcac tggagacgtt ggaggcgttc
     2701 gctgacttgg cggacattgc gttgattgat ctgcgctatg cccgggccga gagcgcggcg
     2761 gaaggttccg atgcgcggac gtacgtcgag gcggcgcgcg gtgcgttgcg gtggttctgg
     2821 gagcgtctgg ggccgctgga gaccgacgcc tcgggaatcg ccgtgcgcgg cgtgatctgc
     2881 cgggttttgg cgttgccggg gcgtattgcc gaagcggtgg agaacctcga gtggctggcg
     2941 cggcatacgg gcagcggcat ccacgtcagc gtgatgtcgc agtacacgcc gctgcaccgc
     3001 gcgttgcaga cgccgggctg ggaccgcaag gtcacggctg acgagtacgc ggtactggtg
     3061 gaggcagccg aagacctcgg attggggaac ggctggatac aggagtttga aggcgaagcg
     3121 cccgccgacc tgctggggca gaatatgccc gcaggcgaag gtgctgtggg tgcgggctcg
     3181 aacacgcccg tgtaggacag tgttgtagag agtcaacgag ccgcatggtg cgcggctgaa
     3241 agggtaacga tgagcggtca tagtaaatgg tcaaccatta agcacaagaa gggcgcggcg
     3301 gatgccaagc gcggtaagat cttctcaaaa atcgccaagg aactaacggt cgttgccaaa
     3361 gccggcggcg gcgaccccaa caccaacccc acgctgcgta ccctgatcac aaaggccaaa
     3421 gcggtcaaca tgccaaacga caacatcgac cgcgccatta aaaagggcac gggcgagctg
     3481 gcggccgacg tccttgaaga gatcacctat gagggctacg cccagggcgg cgtggggctg
     3541 gtagtcaagg tgctgaccga caacaagaac cgcgcggcgg ctgaagtccg gaacatcttc
     3601 aaaaagaacg gttctgattt tgcaactttg ggctcagttt cgcgcggttt cgagcgcaag
     3661 ggtacgatca tgattccggc ggcagagggc ctgaccgagg acaaggtgat ggaggtcgtt
     3721 ctcattgcgg gggccgacga catggaatcc gaagaggacg gtttcaccgt cacctgctcg
     3781 cctgcggcgt tctcggaagt gtgcgcggcg ctggaggcgg ccgaaatcgc gttcgatcag
     3841 gaaaactcac tggtcgggct cgtggcgcaa accttggtgc cggtcaaaga gttggcggtc
     3901 gccaaggcgg tcaacaagtt tgtcgacgcg ctggaagaaa atgacgacgt acaggacgtc
     3961 ttcaccaata tggatatcga cgacgcgctt gtcgagcagc tcgaggccga agcgtaggcc
     4021 ccgatttctt tgcgctttgt ttgccccaag ggcgccctgt ttgtttacgg ggcgcccgtt
     4081 ttcattacaa aaagaagccg cccatcgcag ggcggcagga tcgcataaaa atacgcatcc
     4141 gtgcttaacg caccttgccc tcttttttgc gcttttcgat aatggtctcg gcgagctcga
     4201 agggcacgcc ctcataccgc tcgaacgtca tcgaaaacgt accgcgcccc tgagtgagcg
     4261 tacggatcga attcgaatag ccgaacatct cgcccaacgg aaccaacccg cggatcagct
     4321 tgctcccggc cttttcgtcc attccctcga cccgaccgcg gcgctggcag atcgacccgg
     4381 tcgcggcacc catataatct tcggggaccg ccacctcgac actcatgatc ggctcgagca
     4441 gttgcggatt gcccttgagg aagaggttct tgaagcactg gcgcgcgcac tccacaaaag
     4501 cgaattcgct ggagtcgacc gcgtggtacg aaccgaaatg caccacgacc ttgacatcca
     4561 ccaccgggaa cccgccgcac ggaccgtttt ccatcgcttt gatgacgccc ttctcgaccg
     4621 ccggaatata ctcgttcggg atcacgccgc ctttgacctc attgacgaag gagaagccgc
     4681 gtcccggctc ctgaggttcc agacggaggc agacgtgcgc gtactggccg cgaccaccgg
     4741 actgtttgac gtgcttatat tcgccgtcca ccatatgacg aatcgtttcg cggtacgcca
     4801 cctcgggctt gccgacctcg cacgccacac cgtactcgcg acgcaggcgg tccacaatga
     4861 cttcaaggtg caactcaccc atgccggaaa tgatcgtctc gcccgtttcc tgatcatacc
     4921 ggaccacaaa ggtcggatcc tcttgcgcga gcgagtacag ggcgttaccg agtttctcgt
     4981 tgtccccgcg cgaggcgggc cggatcgcga ttgagatcac cggcgccggg aattcgatcg
     5041 actcgaggag aatcggatgc ttgggatcgc agatcgtgtc gccggtgcgg gtcttagaga
     5101 ggccgaccac agccacgatg tcgccggcat acgccgcctc cagggcctcc tggcgggccg
     5161 catgcatacg gaagagccgc ccgatgcggc cgggctgctc catcgtgctg ttcaagacct
     5221 catcgcccac acgcaacgtg cccgaataga ggcgcacaaa ggtgatcttg cccatgtgct
     5281 tgtccgacag aatcttgaaa gccaacccgg caaacggctc gttctcatcc ggactgcgca
     5341 gattttcggg gttgtccgcc gaacgcacag agccgatgtc agccggagac gggagatagt
     5401 cgatcacacc gtccagaagg ggctgcaccc ccttgttctt gaatgccgtg ccgcaatacg
     5461 tgggcgccag tttacgattg atggtgcctt tccgcaaacc ggatttgatt tcctcaatac
     5521 tcaactcgcc ggtctcgaaa tacttggcca tcaacgcatc gtcctgttcg gcggcctgct
     5581 ccatcatttc gacgcgcaac cgctcacact cctcgcggaa ctcctccggc acctcctcca
     5641 ccagaaccct gacaccgcgg tcctcctcat caaaaacaag agccttcatc gtcagcaggt
     5701 cgaccacgcc gcgaaaagag tccgccacac ccatcggata aaccatcacc acggggttgc
     5761 cgcccagcgt gtaccggatt tcgttcacca cattgaagaa gttggcgccg atccggtcca
     5821 ttttgttgac catgcagagc ttgggcacct cgtatttctc gctctgccgc cacacctgtt
     5881 cggtttgggg ctgcacgccg cccactgcgc agaagagcgc gatcgcgccg tccaacacgc
     5941 gtagcgaccg ttcgacttcc gcagtgaaat ccacatgtcc gggcgtgtca atcaggctga
     6001 tctggtgatc gcgccatgaa accgcggtcg ccgcagaggt gatggtgatg ccgcgctcct
     6061 gctcctgcac catataatcc atggtggcgg caccctcgtg cacctcgccg agtttgtaat
     6121 tcttcccgga atagaaaagg atacgctcgc tggtcgtggt cttccctgcg tcgatgtgcg
     6181 ccatgatccc gatgttgcgg atctggtcca gcggtatggt cctggacata gtcttacact
     6241 ccttgtcgcc ttgctcgcta ttgcctgcgg atcggtcaca cctgacaacc cggatgtgca
     6301 ataggttttt tcacaggcaa ccctacccct gatgggggca aatagaaatg ggtagtgttt
     6361 acttttttcc gattcgcatc aagtgcaaaa gtgaaaattg tttttggcgt ttcctaatcc
     6421 gcgccaatcg ggtattctct tttcatacca gaaggagaca cgatgatccg accctcgtta
     6481 cccgttgagt attttgaacg caaccgagaa acgattctaa aggagtggtt cgccctcttg
     6541 cgtctaccca ctgtgggtac cgacccgaag cgtctcggcg agtgtgcgcg ggcggccgcc
     6601 tggctgaaag tctttctccg taaactggac ttcagcgtgg acatcatcac tccagaaagc
     6661 ggacatcccg taccggtggt actggcggaa cggcccggca acggcacgtc cacggtgctg
     6721 ttctacggcc attacgatgt gcagccggag gatccgctcg aagcgtggct gaccccgccg
     6781 ttcgagccca cgctgatcaa cggacgcgtg tatgcgcgtg gcgcgcaaga caataagggc
     6841 caggtgttcg gttttcttca gggggtcaaa gcattgatcg aagccggagt gcccttacca
     6901 acgctccgca tcgtgatcga aggccaagag gagagcggga gcgaagggct caccgctctc
     6961 gctccctccc tgcgccagcg cctgcaggcg gacgtgatga tggtgtgcga cacctcctgc
     7021 ggtccggaga accaaccggc catcatcgcg ggcctgcgcg gtgtgcagca cttcaccctg
     7081 accctgaacg gaccggcata cgatctgcat tccggcatcc atggcggggt cgcgcccaat
     7141 ccggcccaag gcatggcccg gcttctggcc tctctccaca ctccggacgg tgccattgcc
     7201 gttgccggat tccgggaccg ggtccgaccg ccggaagccg aagaattgga gcacgcccgc
     7261 cgagccgccg cgcccgccga gaattacgag gcggagatcg gctgtccgcc ggtcggcggg
     7321 gatcggcggc tcgatccggt cgcgcgcggc agtttcgagc ccacgatcga gatcaatggc
     7381 atccacggcg gctatggcgg cccgggttcc aaaaccgtga tcccatcaac cgtcctcgcc
     7441 aagttgagca tgcgcctagt gccagaccag cggccggacg aagtcttcga tgccgtgcgg
     7501 agtcatctcg aagcgcactg cccccgcggc ctcaagctca gcctcaccga aatctgtccc
     7561 ggtgcccccg gcttccggct ggagctccac tcccccctgt tccggctggc cgcggatgtc
     7621 ttggaacgtc tggacaaccg cggcgcgctt ttccgctggg agggcgcctc catcccggtt
     7681 gtcgccctac tgcggcaggt ttccggcgcg gccccgcttc tggtcggctt cggtcgtgag
     7741 gaagacaaga tccactgccc caacgaatcc ttcggtctag atcaattcga acgcgtcatg
     7801 acctggtccg cgatgatgct cagcgaactg gcctgactgt ccgaaccggt acgcgcaaaa
     7861 cagacccaaa ccgcgcccgc gcatcgaaaa tgacgaattt ccttgccgtc ccggcagcca
     7921 cgccataaac taacgttgaa tggcaggaag acagtgatct gttattccga tttcgtcaca
     7981 cataacggcg ctacggaaag gggggcgaca tgacaacctt cgatgtccag acaagcctgc
     8041 acagccaagt cgtgaacatc acggattcca tccgggaggc catcaagtct ctcggcatca
     8101 cggacggcgt ggtcaccgcg tttgtcccgc acacgacctg cggcctgacg gtcacagagg
     8161 gcgatgaccc gaccgtgacc gcggatctgt tgcggcagat ggatgaactc gtgccgtggg
     8221 accgtccgta ttacacgcat accggaggca acgctgcggc ccacatcaag tccgcactga
     8281 tcggcacctc ccttcacctg atcgtgaaag gcggcacgct gcaactcggt atgtggcagg
     8341 gaatcttcct gtgcgaattc gacggccccc gcatccggca tgtgtgggtg cggtaagcta
     8401 aagcggatcg aggattccgt ccccgtcttt cgacagatgg aacccgcggc tgtgcgtctg
     8461 cccggccgcg tcacgccagg atacgcggta ccgccccttg aagccgcgga aggcgacccg
     8521 gccgtccggc ccggttttgc aggtcagctt cgtcttccac tcgctttgaa tcagcttgtc
     8581 cagcgcatgg aaggcgggtt tcggctgcat gtcccgggta aagagtcctg acaccgacgg
     8641 ctcgccgggc gcgccgcaat cgtccaccac attccaccag gtgatgccca tcatacgttc
     8701 gatgctgaac cagaggcggt agaggttacg cgcaatcacc gcctggatct tacatccctt
     8761 ttcatcgcct cccggcgagg tgatggtgat ctcgctcagg tgaatcggca gcttggcctg
     8821 ggagagcagc gccatcttgg accagacctg atccggtgac tcaatcggct tgcccgccgc
     8881 gatgtcgagg cactgccggg gattgaagag gtgcatctgc gaacccataa tgtcgatgcg
     8941 gcagccgcgc gccatcaggt ctttgacctg tctcacgtaa gcttcggtgt tgagataatc
     9001 gttgatgttg agtttcaccg ttccgggaaa ctcacgttgc gcaacctgaa acgaggcaaa
     9061 ggtgtagtca ccgggcatca gtccatagcg gctcttgcaa atcacgtccc cggggatcat
     9121 catgcccttg ctgaaatcgg tcgcgctctc gttaaccaca tcccaactgt ggatgcgccc
     9181 ctgatagtgt cgggcgagct cttccacgcg cgttgtgaaa cggcggttga tctcacgcgt
     9241 gtaaacggga agtagcgccg caatctccgc aggggacagc ttctgtaacc cgtccttgcc
     9301 caatttctga atctccgcct tttcctccgc gggacagttg cgctccatca gccactccgg
     9361 atagtgccag gtcttgttcc cccacgtgat cggatgaccg tgcagacgga ttcctttgct
     9421 ttcacagaag gccaccaccg gatccgtagc cgggcgacgc cagtgcggct cctttttcgg
     9481 ttccttcacg ctgttccaat aggcttccgt gtcgcgctcc tcttccgcaa aacggagttt
     9541 tccgggctcc atctcgaatt ttttccagta gaacgcaatc gtcgccgaat tgaacagcgt
     9601 cccatacaac tccttgtact tccggttacg ctcgaaagac ccgagctgat tgaaattaaa
     9661 gatatgcgca ccgaagatga agtcatgcga gagttgctcg accttcacat ccactccggg
     9721 cgccaccccc tccagaacga gcgtcgcatc cgccttgcgg tacttctcga tatcgcggtc
     9781 aatgcggacc tgaacctccg gattccaaag tttccagtac gcctcgctca ttactccgtc
     9841 cagttcggcg ccgcaggcca cccagcccga caagcccagc acgccgatga acgtcccgct
     9901 catcgtgatc ttccggttcg ccttcatcat ccatccgcct ttctgcccac aatcaaagga
     9961 tgcagccaga cgcacgacgc gtcaaagtcc gtccttcatg gctcattcta cagccttcct
    10021 gtccttgcgc acaagtccgt gttcttgatt tttgacgggc accttcactt cagggcggcg
    10081 gtgatcgcct cggcaacccg tttgccgagc aggtcatacc cttcgccttt gaagtggaca
    10141 tccttcgggt tctgtacctt tgagaggtgc ggtgtgatga aggtgaacag gtcgttgatg
    10201 gcgacaccat gccgcgtcat caactcggcc gcaatgcggt tcttctctac gatggcctcg
    10261 gaggccttcg gcccgtcttg ggtatccggc gggatcggcg tggtgctggc ccagatgacc
    10321 ttggcaccgg tcgctttcat gcgggtgacg agcgtctcca gccgctggcg gtaaacgtcc
    10381 ggaggcgtgg cgcggtcatg gatcccgaaa ttgaagtgaa tcacgtccca tttttcgcca
    10441 gcgagccaaa tatcgagttt ctttaacccg ttggccgatg gaccgcagtt ttccggcgca
    10501 cgatggacgt tggctttgcc aaccagcgcc ttgcgggttg ccagtgtgta gcctcgtgac
    10561 acggaatcgc cgatgagcag cacacgcggc aaacccggaa cgtccttcac gtagtcccac
    10621 gcctgttctc tgccacggac gcggccctcc ttgtagcgcg gcaggtagaa gcctcccaag
    10681 ttcgcctgca gcgtgcgctc ccatgcctgt tcctccggcg gcagcttggc gacaatctcc
    10741 gcgtatttcg cgtcgatcgc cgcagccgtg gcgctcttct tgtctgccgc ctccttggcg
    10801 ttcgtgggct cacctttcga ctgcccgcgt ttcgcggact cttgcgtttc gccgggccgg
    10861 accaggtcgg agccccatac gcagtgtggc ccgacgcacg aaaagcagat gcaagcggat
    10921 agcacgatta cgtttttcat tctgtctccc atagcttggt ttgagtttgc ggaaatccat
    10981 caccatccga gaacagcggg tacgcatgga tggctgcctc ttgtctggag agtaagcccg
    11041 cacgccttcg caacagtcgt gcgggtgata cggatagcgc gacgtttcaa actcatgtta
    11101 tcccctcccg aaccggttga cggaattcat cgcgaaaccg acccgaccgt tccattcact
    11161 ggtttcccat ccgcatccaa tacattgacc ggcccccaat cgagccggcg cagcggctct
    11221 tcgacctgag tacggtcccc gacaaccaac cagaccagac tctcgggccg gatgacctct
    11281 gccgccgccc gcgtcacctc cggcaacgtg agcgcgcgca tggttccggc atacacggag
    11341 taatactcct ccggcaggtc gaaccggacc acctgttcga gcgcaccgac gaccgcgccc
    11401 aacgtttccc aacgccccgc gagttgcagc gtatgatgcg ccaccgcctt gcgcaattcc
    11461 tccgcagtcg gagggttcgt ccccgtgatt ccgcggaatt cgcggtccaa ttcgcgcatc
    11521 gcgtccgcca cgcggtcagt ctggatcggc gcgatacaga ggaacgcgcg cgggccgcac
    11581 acttcggtga ggtaggtccg cgcgccatag ctccagtgtt tctcctcccg gagattcata
    11641 ttcacccggc tggtgaaggc accacccaac agcgtgttca gcgcttcgat cacggggtcc
    11701 agcggatggc gcttgggcgg cgcgatcccg ccggcgacaa tcagcgtctg cggcgcggca
    11761 gggcggtcga tcagccagac tgagggtcgg agtgcgggac gcgcctccgc cacggacctg
    11821 tccggaacct ctccgggctt ccatgagccg aagcggcgtt ccaaacgcgg cagcagcgct
    11881 tcgagcgtga catccccggc aaccaccagt gtcgcgcgat tcggcttgaa ttcctgccgg
    11941 tagaagcgct ccagatcatc gcgggtcaac ccggcgaccg actcctcttc gccactaccg
    12001 ctgaacccct gtccgtacgc gtgccgctca ccgtaaagca ggcgcggcat cacccgcatc
    12061 gccaccccgc ggggattagc cttttcccgc cggatctgcg ccaaccgctg gtgccgcagc
    12121 cgctggaaat cctcctcggg aaaggcgggg tggaggatca cttcagcata gagatccaag
    12181 gcgcggtcta aatggcgcga gagcgtgttc agcgagacca gcgtcgcgtc aggcgcgcac
    12241 cccgtgctca acgccgcgcc caaccgttcc aattcccgtc cgagttcaag tgccgagagg
    12301 tgccgcgtgc cttcatccag cagatccgcc atcagtgtgg ccgtaccgcg ctgagcgcac
    12361 gcatccgccg caaacccagc ccccacactc agcgtacaaa gtaccagcgg agtttcatgc
    12421 cgctcgacca gcaccacccg caacccgttg gagagcgtgg cccgacgaac cgcaggaaag
    12481 gcggcctcgg gcagttccga tggcagcggc agcgcgctgc ggtctgctcc ggccgtggcc
    12541 acgcgatacg cggggaacgg ctcgacctcc agaatgaagg caccatcgga cagccactcg
    12601 cgtgccgtct caaccacctg ccggggtgtg gcctcgcgcc attcccgcat ccgcttttcg
    12661 tgataggccg catctccggt gaacagcaga tcacgaatca gcatgtccgc tttaccgcca
    12721 acctgttcgc tttgccgaat gaaatccgcc tcaatacgag cttggatacg ttccaattcc
    12781 ctgcgagtcg gcccctcgcg gagaaaccgg gcgatctctt catcagccgc ccgctcggcc
    12841 tctgccagag aaacgcccgg ccgaacggtg atgaccaccc cgaactgccc cgcgatctcg
    12901 cgcagatccg cccaagcgtg aacctcggtg gcaagttggg tgtcatacac cagacgcttg
    12961 taaagccgtg aggatttgcc tgccgcgagc gcctcggcag ccaaggtgag acggttgcct
    13021 tccgtcgtgc cgtattgcgg gatagtccag actttgtaga ggcgtgcctg cggcacacgg
    13081 tcctgcaccc gcatgcgccg ggccccggtg cgtcgcacca catccacgac ggggcgcgcg
    13141 accggcggtc ccggggggat gtcgccaaag gcgtgccgga ccttctgcaa cgcctcctct
    13201 gcggagacat cgcccgccac aaccaacacc gcgttggccg caccgtaaaa atcacggaac
    13261 cacgcgcgga catcctgaag tgatgcggca gcgagatcca cctccgaacc gatcacctcc
    13321 catgaatacg gatgccccaa aggccaagcg tgccgcgtaa tgagtcgcca ggcgatgcgg
    13381 tacggctggt tatccccctg ccgtttttcg ttcagcacca ccccccgctg ctcctcgagc
    13441 cgctcttgag tcaacgcgcc aagaaagtgc cccatgcggt cggactccat ccagagcgcg
    13501 gtatccaatg cttcgcgcgg caccgtctga aaataatggg tgcggtcttc attggtcgtc
    13561 ccgttgatcc cggtcgcgcc gacccgttca agcgccttga aataatcgtc gtcgaaatgt
    13621 tcgctgccgt tgaacatcag gtgttcgaac aggtgcgcga acccggtgcg gccgggacgt
    13681 tcgttcttgg atccgacatg gtaggcaacc tgcacagcca cgatcggcgc cttgcggtct
    13741 tcatgcacca ccagtcgcag accgttctcc aacacgaaga cccgatgggg tatcgcaccc
    13801 gccgcagcac accccgcgac aacggccagc atgcatgcaa tcccgatgct gcgttgccga
    13861 ctccccatac cttacgctcc cgaactcttt tgctgcgcgg accgtccctt gccgtcgtcc
    13921 acgtccacga cctcaccccc gaagagttgc atgacctgtt tcagcgcagg gtcatcgtgc
    13981 gccacacccg gcaacggccc agcagcggcg gcaccggact ggacggaact cgccgcggac
    14041 ggcgccgcag cgggggtcgg gtccgacccc cgcaacgcgt tgagccgttt gagcacctcc
    14101 tccagcgaga cggcccgcgc gatacgcgcg caacggatca gcgacaactc gatcaaggtg
    14161 cgcatggaga gcgcaaagcg caggcgtccc tccatctccc cgagttggtc agcgatctgc
    14221 aacacccgtg acgaatccgc gtgtgcggcc tgcgcagcca gactgcggat ctgttccggc
    14281 gtggcatcga gtgtggacat ctgctcgccc acctgctgat acaccagcag gttgcggaag
    14341 tgtcccatga gctccaccac caaccgccgc atgtctttgc ccgcactgtc gaactgatcc
    14401 accaaccgca aaatttcggc catgtcaccg gtcaacacgg cagttgcgag cccttccagc
    14461 gaggcgcggg agaccagccc gaacaccgcg agcacatcct cttcagtaag cgccccgcct
    14521 ttaaacgaga tcagctgatc gagcgcggac agcgcatccc gcatgccgcc ttcggcccca
    14581 cgcgcgatcg ccagcagggc gtcctcgtca atacggatgc gctcggcgtt acagatatgg
    14641 cgcaaccgct cgacaatcag cggcgtctgg atacggcgca gatcgaaacg ctggcaacgt
    14701 gagatgatgg tcggcagcac cttgtcgcct tcggtcgtcg caaaaatgaa tttcacatgc
    14761 ggcggcggct cttcgagcgt cttcagcaat gcgttgaacg cggccgtgct cagcatatgg
    14821 acttcgtcaa tgatgatgat tttgaagcgc ccgtgcgtcg gcgcaaactg gatctggtcg
    14881 cgcaaatcgc gcacctgatc gaccccgttg ttggaggcac cgtcgatttc cagcacatcc
    14941 agcgcattcc ccgccgcgat ctcgcggcag agcggacatt cgcaacaggg tgtggcggtg
    15001 gggcctttct cacaatcgag cgccttggca aaaatccgcg cgagggaggt cttcccgatc
    15061 ccgcgcgggc cgacaaacag atacgcgtgg gccacgcggc cgctttcgat cgcgttcttc
    15121 agagtacggg tgacgtgctc ctgaccgacc acatccgcga actgttgcgg acgccatttg
    15181 cgtgcgagta cttcataagc cataacggaa aacagtttac cgtttacccc gagggaagca
    15241 aagggaatct cgcgccttcg gcaccgagtc ttggaaggac acatcgtccc tttgcgtctt
    15301 tttttgtcag cggatgatca gcaggaaatc cagaagcggc ttcgtctcgt tcaggacacc
    15361 gttgatcacc tgaacccgac gcccggtcag gcggcggatc gtgaacgtgc cgccgcccag
    15421 atcaaggacg gcatcatcgc tcagcaccag atcaaggttc ggggcaccga aggacgcgcc
    15481 tgcgcgcaca aaagaaacgc ccgagagcgt cagggtcgcc acgcgttcaa cgctgttcgt
    15541 caacgtgccg gagagatcga gcgacgccag cgtctggtcg ttgccgttca ggtcaagcgt
    15601 cgctgcagaa gccacggtca ggtccgtccc cgtcggcagg acgtccgcat acttcgccgg
    15661 atccggcagg tttttgagtc cccacttcgc catcaagtaa tcttcgacca gactgcgttc
    15721 cgtctccccg agcacccggc catagacgac gatctcgcag atgtcgccgg acatcggacg
    15781 cttatgcggc gaatagtcac tgagagccgg cgagtcccat ttcatattgc cggggtaaac
    15841 gcgcgaaaac agatgtggcg tccccttggc gaacgcactg cccgcgtcgc cgttgacacg
    15901 gtcggtcagg gcgatcggat cgctgtagga cgtgccgttg tggatacgga tgcccgcgtc
    15961 cacgccgccc tggccgaaga cgccgtcaag tatcccaaag ggcgtcgcat catgtccgtg
    16021 cgcgacgaca ccgaaatacg tgcgtaagct gcagggcacg ccgccggcca gatatcggat
    16081 gtgggaatga tcgaaataga ccgttgacag gccgttgatg tggttcgtct tgtaggtcgc
    16141 ggcgttcggc gtcgacaggg cctggtagtt cacattcgag aagatgatgt ccttgctgtc
    16201 cgcgtcggcc catttcacca cgcggtccga acccgcttcg catgtcacgt aacgccacgg
    16261 cttgtcaatc ctgcggccgc gtgttatggt ggtgcacgta att
//
LOCUS       DAOSLE010000059        16181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3767_length_16181_cov_7.609575, whole genome shotgun sequence.
ACCESSION   DAOSLE010000059 DAOSLE010000000
VERSION     DAOSLE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_16181_cov_7.609575"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK576_05675"
     CDS             complement(<1..565)
                     /locus_tag="PK576_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOE61118.1"
                     /translation="MTQKKLVTLIITAVVLVGLAYLTSSSRKMKTPSLVGKPVVRTFD
                     LSEVAKIEAGSQNGAKLMLESTDTGWVIRSLFGYPADIAKIRENLLKLKDLKAGHVAV
                     GKKLENPVLVDLQNASGKSLASLRLGDKHMRQPSGEMSQFGGGGYPDGRYVAGETDDT
                     VVLVKETLDAFDGDVKSWTDTQIASVPS"
     gene            complement(651..2561)
                     /locus_tag="PK576_05680"
     CDS             complement(651..2561)
                     /locus_tag="PK576_05680"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOE61119.1"
                     /translation="MSDKQTKGFSRIGTGAAGLAALLVILGAANLIIANLRLRVDLTA
                     EKLYTLSQGSRQVLGKLENDVVLKFYFSASSADMPMGLKTYATQVQDLLKEYEIAGRG
                     RITLEAYDPKPDSDSEEWAQRYGIEPQQTNPFGQPVYFGLVAVSGDQEQVIPGFNPRT
                     EATLEYDITRLITRAAWPEKPVLGVLSSLSVLGSPQNPMMMMQRRQQQDQGWMAFREL
                     RKDYVVREVRPDTETIDNDVKTLIVVHPKDLSDQTLFAIDQFVLRGGRLVVCVDPFSI
                     ADFEANQQQNPMMMQMGGGQAGPSTLGKLFDAWGVSFDTSKVVADLSCATKLNAGNGR
                     IEENPAFLSLGTANMAKDDLLTAQLSQVMMPFAGALSANTPKEIIFTPLITTSRDHAC
                     LVDQMNAQFGMGAMRAQLKPDGAQRVLAARLHGTFKTAFPQGVGTNGTDQAAATNAAP
                     NHLTSGTSTVMVFGDTDFLNDRFCVQIINSLFGSIAQPINDNLTLFGNTVEQFAGREE
                     LIGVRSRGQFNRPFVKVDQLEVKAMRQWQAEEERLEAALQETQQRLTELQQRKSGNER
                     LILSKEQQAELEQFRKTQVETRKQLKKVRKNLNSEIERLGLFLKVLNIALVPLLVIGF
                     GVQRGLRRRGRR"
     gene            complement(2610..3356)
                     /locus_tag="PK576_05685"
     CDS             complement(2610..3356)
                     /locus_tag="PK576_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE61120.1"
                     /translation="MCNVKNIKSIFEREFKSYFDSPVAYVFLVAFLVLIGFLTFGVAM
                     FYERRQANLMPFFFWHPWVYLLLVPAATMGLWAEERRTGTIELLLTLPVTLAETLVAK
                     FLAAWAFIGIALALTFPIVLTTAWLGNPDMGAIFCGYLGSLLLSGACVAIGVFASALS
                     RSQVIGFVIALAICLFMLIIGFDPVINAVAGWGVPAGLVDAVASCSLMRHFDAMRRGV
                     IDFHDIGYYVSVILFMLAAAHVVTDNRKAS"
     gene            complement(3349..4080)
                     /locus_tag="PK576_05690"
     CDS             complement(3349..4080)
                     /locus_tag="PK576_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61121.1"
                     /translation="MLTVSNLKKNFGPIQAVKDVSFTVEKGEVLGFLGPNGAGKSTTM
                     RMITGFIPPTAGTATICGHDILTEPVAAKACIGYLPENAPSYHTMTVTDFLTFIGKVR
                     GYTGQELKDQVESVIKKSRLETVRNQTIETLSKGYRQRTCFAQAILHDPPVLIMDEPT
                     DGLDPNQKFVVREMIKEMAADKAIIVSTHILEEVDAVCTRVIVIADGEVVANATPAEL
                     RAQDPQGRLDVVFRNLTMTEGVSHV"
     gene            complement(4207..5580)
                     /locus_tag="PK576_05695"
     CDS             complement(4207..5580)
                     /locus_tag="PK576_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE61122.1"
                     /translation="MNTKPNLLIIEDDPDNARSVTEAAEDAGFATVCVANGKAGVAAF
                     RNQIPDLVLSDLVLPDMDGLEVLARLRKIDDMVPVMIMTAYGSVESGVRAMREGAYDY
                     VTKPLDLDDIQSKLRRACEISRLRRQVDKLSRSVREKYASTAILAESPGMKKVIRQIE
                     ALADTSATVLICGESGTGKELVARALHVDSKRADGPFVAVNCGAFAENLLESELFGHE
                     KGAFTGAIGMYKGAFERADGGTLFLDEIGDAPASVQVKLLRALEEKEVRRVGGKEVFS
                     VNVRLVSATNRDLDALVREGTFREDLFYRLNVVTLRIPPLRERREDIRPMTDRFIARA
                     AVENNKTITCVRPEFYAALEAFSWPGNVRQLRNVVESAVLLSPDGELDADSAALPDEK
                     ARADPDGAAPQGLTFPQDMTLEQIERAALEEGLRRNNGNRTLTADQLGLSRRTIQRKI
                     KEHNLPY"
     gene            complement(5588..6988)
                     /locus_tag="PK576_05700"
     CDS             complement(5588..6988)
                     /locus_tag="PK576_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE61123.1"
                     /translation="MRQSDRPVNRGARGGWRLELILLSALLTSAFAAGCVLVLLKGRH
                     DRQAHLAATATVLQEGEALLQALCDLGDCSSTHPSPAEWRRFSTVVDSAFAVRKDVQS
                     VSVSRNGITVFQRQAGGLHPPQGESAESRAREKERRVETEIAPGTLEINGEPQSVFVI
                     TRTQTLDDGGRVVTEATFRREAVGAEERTARSLVSSLFWFSLAVVFLSFALCAAVLVF
                     FVARDRRREQRARREEHLAFSGVLANGILHDFRNPMSAVRLDAQMLGREMARADGFRA
                     ERVRDLAERIARTMARMDKVFQEFLFLAKPADEQPETVRIEQVVRECVDTLAPRLEQA
                     GVHVAIHAPDTMPAVSAFPFALRRALLNVLMNAIQFAPEGSEITATIEERGRHIVLDV
                     LDRGPGIPPAEREKVFEMFFSGRPGGTGLGLFLARTAVRRCGGDIQVLTREGGGADLR
                     ITLPVAPPSSPPPSDV"
     gene            7409..7936
                     /locus_tag="PK576_05705"
     CDS             7409..7936
                     /locus_tag="PK576_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61124.1"
                     /translation="MRNAKFIGRAWAALDYFLLVAVLGLLVTLAFLGRSVGEAAADGN
                     GEISGGTEPVERVRPFKSRVVEGLRVSAGRQRLDVIAQSMAASDWDRLPASPALDMRE
                     NGQAYEILFSLPSGVDRDNVRVTSSGSLLTLALTDPGTGKVLMRRIRIPCEAEPNSRI
                     HSVVSNRVLHVRIYP"
     gene            8064..10754
                     /locus_tag="PK576_05710"
     CDS             8064..10754
                     /locus_tag="PK576_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE61125.1"
                     /translation="MKPIVRLFLTGLFVLSGRVAAQQASAVAANMEDMGNPVVRRNKV
                     KERMRVERMRREEAEAFARSFGIPVRGQRPNGGAWELAAIEGGKPIYRMTLNANAAIS
                     TGANILRLAPYGADGAGDTVGVWDACSARTTHREFGGRVVSRDGAGVVHDHSTHVVGT
                     ICAAGIDSLAKGMAPAVLVDSYDWNDDQGEMTMRGAAYPGEADKIKVSNHSYGMVAGW
                     GYSGNPKFTWYGTGFTASAIEDDFGKYNTYAHEMDKLAFDLPYYLVFWAAGNDRGPSE
                     NPVNGDTVALSAGGIPVTYDSSLHPPADHVYRNGYDTLGYSALAKNVVTVGAVTDAVS
                     GGMRSVANANMTIFSSWGPADDGRIKPDLVANGDRLKSTSYGGDSSYSYSSGTSMACP
                     NAAGTAQQLVHLFANLFSNQVMRASTLKALLIHTADDLGNPGPDYVYGWGLLNGKAVA
                     DLLLDYRAQAGRRQVIENRVSTDRNAISFEMIWDGASPVRATLCWTDPPGVATGIGDS
                     RQPRLVNNLDLRVIAPDGTVHEPWVMPFVGEWSVASCAYPAITGSNTTDNVEQVLIAA
                     PVVEGTYTVRVTFAGTLTNGQQPFSLIVSGGVPEQQAPRPVLISSSPGSGEGVLPFTF
                     TGDHFLLGATARLRRLGQPDALAMNMQVLGDVAYARVNTVGMSSGWWNLTLANPDGGR
                     ATLYNAFVVPELFWSETFETNDLLSRGWSFLQTLGTNQWELSTVKSVSPSNSAFSLGV
                     DTRSDTSLVSPPIEIPLDQTALQLSFWHDFSFSANDAGVLEFSLDNGDWYDVTDIGSG
                     VVFSQNGYTGTVAGKGAPNSLNPLVGRSAWVGTSGGFVPVTLSLTDTARYAGRQLRVR
                     WRLGTDNSTASEGWYVDDVRGLGVSAPPPVPPDGTVFGVR"
     gene            complement(10811..13285)
                     /locus_tag="PK576_05715"
     CDS             complement(10811..13285)
                     /locus_tag="PK576_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE61126.1"
                     /translation="MTPELLAPAGGFEAACAAFQYGADAVYLGLPRFSARADAEHLTP
                     DRLRLLKAYAQSFTPAKRVYVTFNTLVTESEWEPALDLLAVLDDLTPDGVIVQDLGVA
                     RLIRDHFPRLALHASTQLATHNLEGALALRELGFTRVVLARELTLDEIRRISQESGMQ
                     TEIFIHGALCYSYSGLCLFSSLATGRSGNRGRCAYCCREPFTAAECASDNAAPHFPFS
                     MRDLCLAPLLDDVTGSGAHALKIEGRMKSPLYVACVTDYYRRKLDRTLSPETERERLQ
                     DLQTIFSRSGTQLYAQDPHAQPETIIDPLTVGHRGARIGEVEAVIRDRDGTRWLRFRS
                     DRALEKHDGLQLEFPGEGRPPGFAVMRLRQAGTRRPALSLAAGERVEVALPEGPFPPI
                     VRGTPVFCSASQAVRRRYGIQSVRESGLTTGRPVDFEVRLDPGGIAVRASTPCEPCGQ
                     VTAETQIALPLSPSRQPEQSPAAVQKAFSRLGDSAWRLGTLTFHDPDGRYAPPSQLNA
                     CRRDVLVRLNGAWDQHAATRREAILRKWRPEPAIPSPSPDAAPLPHWTLKHRADAQPL
                     PTAALSGLSTLILRIGHAPADALKRQLEAWREAVPQTAICPALPVITRACATREMRDA
                     LRVLAREGWERWECADLSGWRMLTEEGLSPYSADWSLYALNRAACAEYARLGFAVQVL
                     SPENGLDNIRALCHIPACAGHRPEAEMPVYLHPPLFLSETAPLTGGRPPPDKGRTRDI
                     RIANRRGKTFTVLVRDRLWVTTADEPYCLADRLPELHAAGVGRFRIDLSWSPETIDAE
                     RTIRNVMAGKLPQSHVLRGNFDRGLA"
     gene            13677..15986
                     /locus_tag="PK576_05720"
     CDS             13677..15986
                     /locus_tag="PK576_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61127.1"
                     /translation="MRKTDLSGEWNLYKRGSKKSIPAHVPGCVHADLMAAGEMEDLVR
                     GTRLEAARWVMGEEWVYEKTFVQEDVSAYDRVVLCFGGLNGCAAIRLNDAELGQVTNP
                     FETVEFEVKPLLKAGKNRLRVTFSPPTSTVPGAEEGPNQDFISLSPTVGIGRTVSLLA
                     FTHVRVSEVHIRQDFATAGVVKLHVAVASERYDPAMHLELLVRVCYKGNILHEARDLL
                     NRDRMTLSLTVKNPQLWWPAGLGEQPLYEVTVDVLSGRTCYEHVARRIGLRQVEMEPV
                     RGRAFGRVKVNGQSVFLKGASWVPADLYPARLTRVEYARLVKAVAVANMNCLRVWGGG
                     LYENDAFYDLCDEYGICVWQDLMLGGSRGAPPAKGERASFEREVRSTVMALRHHPCLA
                     LWCGGDGGGKAIDSAYTKIAVDGVRAADPDTPCLPPIPHQPFSLTEPAGYMTMPSFPE
                     PRVIAEYLDEGERNAGHPACAFHMSPSDGARRIYHGFLDHFLVPSSFDHVAWLSQIQQ
                     AVLLRRAYERVRTAKEASPGFLYWHFNDCWPTCSPSAVDYAGRWKALQYLARRFFAPI
                     SLCGVYCRESGTVTLNVFHDGLRPTFKGEMQWRVTQMEGIVVAEGTKKVSVAPLSRDE
                     SVRVKVADILKRCGADNLIMWIYLLDEQGNQVAWNTVLFCEPRALKLQAPRMRAEIRA
                     WDDNSFAVTLTSHHPALWVWISLDGMDARMDDNFFSLEPDKPFRVRVIPATRMKLDAF
                     RQILRIGSLRDTWQDKRLLMQVMAAAKKK"
BASE COUNT         2988 a         4613 c         5338 g         3242 t
ORIGIN      
        1 ccgatggcac cgaggcaatc tgcgtgtcgg tccagctctt gacatcaccg tcaaacgcat
       61 cgagcgtttc cttaaccaga acaacggtgt catcggtctc tccggcgacg tagcgtccgt
      121 ccggatatcc gccgccgccg aattgcgaca tctcgcctga gggctggcgc atgtgtttat
      181 cgccgagacg caggctggcg agcgatttgc cggaagcgtt ctgcagatcg accagcaccg
      241 ggttctccag ctttttgccg acagccacat gtcccgcctt aaggtcctta agtttcagca
      301 ggttctctcg gattttggcg atgtcggcgg gatatccgaa gagcgagcgg atcacccagc
      361 cggtgtccgt gctttcgagc atcagcttcg cgccattctg ggaaccggcc tcgatcttcg
      421 cgacttcgct gagatcaaag gttcggacca caggcttgcc caccagcgaa ggggttttca
      481 tttttctgct ggagctggtg aggtaggcga gtccgaccag cacgaccgcc gtaatgatga
      541 gggtgacgag tttcttttgt gtcatgatac gaatccttgt tggagaggtt acgggacaaa
      601 agggatgcgt cgggaagccg ggacggctgt gccgccccgg ccccgtcgga tcagcggcgc
      661 cctctacggc gcagtccgcg ctggactccg aagccgatta ccagcaacgg caccagggcg
      721 atgttcagga ctttcaggaa caggcccaga cgctcgattt cgctgttcag gtttttgcgc
      781 acctttttga gctgcttgcg cgtctcgacc tgcgttttgc ggaactgctc cagttcggcc
      841 tgctgctctt tcgagaggat caggcgctca ttgccgctct ttctctgttg cagttcggtc
      901 agacgctgct gcgtctcctg caacgcggcc tcaagacgct cctcctcggc ctgccactgc
      961 cgcatggcct tcacttcgag ttgatccacc ttgacgaacg ggcgattgaa ctggccacgc
     1021 gaccgcacgc cgatcagctc ctcccgtccg gcgaactgtt caaccgtgtt gccgaaaagg
     1081 gtcaggttgt cattgatcgg ttgcgcgatg gagccgaaca gcgagttgat gatctgcacg
     1141 cagaaacggt cgttcaggaa atcggtgtcg ccgaaaacca tcaccgtgct ggttcccgaa
     1201 gtcagatgat tcggcgcggc attggtggcg gcagcttggt ccgtgccgtt cgtgccgacc
     1261 ccttggggga aggccgtctt gaaggtgccg tggaggcgtg ctgccagcac gcgttgcgcg
     1321 ccatcgggtt tgagctgggc gcgcatggca cccatgccga actgggcgtt catctgatcc
     1381 accagacagg cgtgatcgcg ggaggtggtg atgagcggcg tgaagatgat ctccttgggc
     1441 gtgttggcgg acagcgcgcc cgcaaagggc atcatcactt gggacagttg cgcggtcaga
     1501 agatcgtcct tggccatgtt cgcggtgccc agcgagagaa aggccggatt ttcctcgatg
     1561 cgcccgttgc ctgcattgag tttcgttgca caactcaagt cggccacgac cttggaggtg
     1621 tcgaagctga cgccccaggc gtcaaacagt ttgccgaggg tggagggacc ggcctgtccg
     1681 ccgcccatct gcatcatcat cggattctgc tgctggttag cctcgaagtc cgcgatgctg
     1741 aaggggtcga cgcagaccac gagccgtccg ccccgcaaca cgaactggtc gatcgcgaag
     1801 agcgtctgat cggagaggtc cttcggatgg accacgatga gggttttgac atcgttgtcg
     1861 atcgtttccg tgtcgggacg aacctcccgg acgacatagt ccttgcgcag ttcgcgaaaa
     1921 gccatccagc cctgatcctg ctgctggcgg cgctgcatca tcatcatggg gttctggggg
     1981 gaaccgagca cgctcaggct gctcagtacg ccgagcaccg gtttttccgg ccaggccgcc
     2041 cgggtgatga ggcgcgtgat atcatattcc agcgtggctt ccgtacgcgg gttgaagccg
     2101 ggtatcacct gttcctgatc cccgctgacc gcgaccagcc cgaaatacac cggttggccg
     2161 aaggggttgg tctgttgcgg ctcgatgccg taacgctgcg cccactcctc ggagtccgag
     2221 tcgggtttcg ggtcataggc ctccagcgtg atgcgtcccc gtcccgcgat ctcatactct
     2281 ttgagcagat cctgcacttg cgtggcatac gttttgaggc ccatgggcat atcggcggaa
     2341 cttgcgctga agtagaattt cagcaccaca tcgttctcca gtttgcccag cacctgccgc
     2401 gaaccctgcg agagggtata gagcttctca gcggtgagat ccacccgcag gcgcaggttc
     2461 gcgatgatga ggttggcggc gccgagaatc accagcaggg cggcgagacc cgcggcaccg
     2521 gtgccgatgc gtgaaaatcc ttttgtttgc ttgtcagaca tagctgtacg ctcccaatac
     2581 gtgatggctg tgttacgatt tcgatgggct tacgaggcct tgcggttgtc ggtgacgaca
     2641 tgtgcggcgg ccagcatgaa gaggatcacg ctgacgtagt agccgatgtc atggaagtcg
     2701 atgaccccgc gccgcatggc atcaaaatgc cgcatcaggc tgcacgacgc gacggcatcg
     2761 accagcccgg cgggtacgcc ccaaccggcg acggcgttga tcacggggtc gaacccgatg
     2821 atcagcataa acaggcagat cgccaaggcg atcacgaagc cgatgacctg gctacgcgaa
     2881 agggccgagg cgaagacccc gatggcgaca caggcccccg aaagcagcag cgacccgagg
     2941 tagccgcaga agatcgcccc catgtcgggg ttacccaacc atgccgtcgt cagcacgatc
     3001 gggaaggtca acgccagcgc gatgccgata aaggcccagg ccgcgagaaa cttggccacg
     3061 agggtctccg ccagcgtcac cggcaaggtg agcagcagct cgatcgtgcc ggtacggcgc
     3121 tcctcggccc agagccccat ggtggccgca ggcaccagca gcagataaac ccagggatgc
     3181 cagaaaaaga agggcatcag gttggcctgg cgccgctcgt aaaacatggc cacgccgaag
     3241 gtcaggaatc cgatcagcac gagaaaggcg accagaaaca cataggccac cggcgagtcg
     3301 aaataggatt tgaactcgcg ttcgaaaatg gacttgatgt ttttaacatt acacatggga
     3361 aacaccctcc gtcatggtca ggttgcggaa caccacgtca aggcgtccct ggggatcttg
     3421 cgcgcgcaac tcagcgggtg tggcattggc gaccacctcg ccgtccgcga tcacaatcac
     3481 ccgcgtgcag accgcgtcca cctcctccag aatgtgggtc gaaacaatga tcgccttgtc
     3541 tgcggccatc tccttgatca tctcgcgcac cacgaatttc tgattcgggt ccagcccgtc
     3601 cgtaggctcg tccatgatca gcaccggggg gtcatgtaaa atcgcctgag cgaaacaggt
     3661 gcgctgccga taccccttcg aaagcgtctc gatggtctgg ttgcggaccg tctccagacg
     3721 gctcttcttg atcaccgact caacctgatc tttcagttcc tgacccgtgt atccccgcac
     3781 cttgccgatg aaagtgagaa aatctgtcac agtcatggtg tgataacttg gcgcattctc
     3841 ggggagatag ccgatacagg ctttggcggc aaccggttcg gtcaagatat catgaccgca
     3901 gatcgttgcg gttcccgctg ttggcgggat gaacccggtt atcatccgca tcgttgtgga
     3961 tttgcccgcg ccgttcggcc cgagaaaacc aagaacttcg cctttttcga cagtgaacga
     4021 tacatccttc accgcctgga tcgggccgaa gttcttttta aggttactta cggtcagcat
     4081 cctgactcct cttcgtcttg ggttgttggt tgaaacagga tcccttgcca gagcagaggg
     4141 atgacaaaaa atcacgtcga aagtagcaga aagtgtgcca ttttttccac ttcatggggt
     4201 acacgctcag tacgggagat tgtgctcctt gattttacgc tggatggtgc ggcgcgagag
     4261 gccgagttgg tctgcggtca gcgtgcggtt gccgttgttg cggcggaggc cctcctccag
     4321 cgccgcgcgc tcgatctgtt cgagcgtcat gtcctgggga aacgttaggc cttgcggtgc
     4381 cgcgccatcg ggatccgcac gcgccttctc gtcgggcaaa gccgcggagt ccgcgtcaag
     4441 ttcaccatcg ggcgagagca gcacggcgga ttccacgacg ttgcggagtt gccgtacatt
     4501 gccgggccag gagaaggctt cgagagccgc gtaaaattcc gggcggacgc aagtgatggt
     4561 cttgttgttc tccacggccg cccgcgcgat gaaacggtcg gtcatcgggc ggatgtcctc
     4621 gcgccgttcg cgcaacggcg gtatgcgcag ggtcacgacg ttgaggcggt agaacaggtc
     4681 ttcgcggaag gtcccttcgc gtaccagggc atcgagatcg cggttggtgg cggagaccag
     4741 ccggacattc acgctgaaca cctctttgcc gcccacgcgc cgcacctcct tctcctccag
     4801 ggcgcgcaac aacttgacct gcacggatgc gggcgcgtcg ccgatctcgt ccagaaagag
     4861 cgtgccgccg tccgcgcgct cgaacgcgcc tttgtacatg ccgatcgcgc ccgtgaaggc
     4921 tcccttctca tggccgaaga gttcggattc gaggaggttt tccgcgaagg cgccgcagtt
     4981 cacggctacg aacggtccgt ccgcacgctt ggagtcgaca tgcagggcgc gggcgaccag
     5041 ctctttgccg gtgccgctct cgccgcagat cagcacggtg gcggaggtgt cggccagcgc
     5101 ctcgatttgc cggatgacct ttttcatgcc gggcgattcg gcgaggatcg cggtggaggc
     5161 gtatttttcg cggaccgacc gggacagctt gtcgacctgc cgacggaggc gcgagatttc
     5221 gcaggcgcgc cgcagtttcg attggatgtc gtccagatcg agtggtttgg tgacgtaatc
     5281 gtaggcgccc tcgcgcatgg cccgcacacc ggactcgacc gaaccgtagg cggtcatgat
     5341 catcacgggg accatgtcgt cgatcttgcg caggcgggcc aaaacttcca ggccgtccat
     5401 gtccggcagg accaaatcgc tgagcaccaa gtcggggatt tggttccgga acgccgccac
     5461 gccggccttg ccgttggcga cgcagaccgt ggcaaacccc gcatcctccg cggcctcggt
     5521 cacggaacgg gcgttatccg gatcgtcttc aatgatcaga aggttgggtt tggtattcat
     5581 aagaggatta cacatcggag gggggtgggg aggacggggg tgccacaggc aaggtgatgc
     5641 gcagatcggc ccctccgccc tcacgcgtaa ggacttgtat gtcgccgccg cagcgtcgca
     5701 ctgcggtccg tgccagaaag agccccaacc cggttccgcc gggacgtccc gagaaaaaca
     5761 tctcgaagac cttttcacgc tcggccggcg gaatgcccgg accgcggtcc agcacatcca
     5821 gcacgatgtg ccgcccgcgc tcctcgatgg ttgccgtgat ttcactgccc tccggggcga
     5881 actgaatcgc gttcatcagg acattcagca gcgcccgtcg cagtgcgaag gggaatgcgg
     5941 agacggctgg catcgtgtcc ggagcgtgga tcgcgacatg gaccccggcc tgttcgaggc
     6001 gcggggcgag ggtgtctacg cactcccgca caacctgctc gatccgcacg gtttccggtt
     6061 gctcgtcggc cggcttggcg aggaaaagga actcctgaaa caccttgtcc atccgcgcca
     6121 tggtgcgggc gatacgctcc gccagatcac gcacacgctc cgcgcggaac ccgtcggcgc
     6181 gggccatttc ccgtcccagc atttgggcgt cgagacgcac ggcggacatc gggttgcgga
     6241 agtcgtgcag gattccgttg gccagaacgc ctgaaaaggc gaggtgttct tcacggcggg
     6301 ctcgctgttc gcgccgccgg tcgcgcgcga caaagaacac cagcaccgct gcacagagcg
     6361 cgaaggagag aaacaccacc gccagcgaaa accagaagag cgaagagacc agcgaacgtg
     6421 cggtccgttc ctctgccccg accgcctcgc ggcggaaggt ggcttcggtg accacccggc
     6481 cgccatcatc cagcgtctgg gtgcgtgtga tgacgaaaac cgactgcggc tcgccgttga
     6541 tttccagggt gccgggagcg atctcggtct cgacccgccg ctctttttcc cgggcacggg
     6601 attccgcgct ctccccttgc gggggatgga gtccgcctgc ctggcgttgg aaaacggtga
     6661 tgccgttgcg cgaaaccgaa acggactgga cgtccttgcg gaccgcgaag gcactgtcca
     6721 caacggttga gaaccgccgc cattccgctg gcgagggatg ggttgaagag caatcgccca
     6781 gatcgcacaa cgcctgcagc agcgcttccc cctcctgcag cacggtggca gtcgccgcca
     6841 gatgcgcctg ccggtcgtgc cggcctttga gcaagaccag cacgcatcct gcggcaaacg
     6901 ccgaggtcaa aagcgcgctt aagaggatga gttccaaacg ccatcctccc cgtgcgcccc
     6961 gattgacggg tctatcgctc tgtctcatgc ggtttttgcc tcagcaggga acgtgccggg
     7021 gtcggggtgc ggcatgtgcc gcgccgcctg accgtttccg gttcctgaaa aaaattgccg
     7081 gattataagg gttcacgcgg aggaagacaa gcggaatgcg acaaattgac gcgtgcatcg
     7141 cgtcagattg tcgcgtgcgg tcaaatcttg gatggtttgg gtccgccacc cggatggatg
     7201 cgccgtgcag gatcgatggg atcatttcaa ccgttcgact ccaaaaggaa cagttgtttt
     7261 tcacacaaag ggtaaaaagg ggaatggggt gtcatgaaaa acctgtgtct tctttgtatg
     7321 agcctaaatt gagttgcggg cttcgtccgc gttaggtgtg tggcatatga attgcttggg
     7381 ttaatacgga gcaaattgag gaatgaccat gcggaatgcg aagttcattg gaagggcatg
     7441 ggcggcgctg gactactttt tgttggttgc cgttttggga ttgttggtca cgcttgcctt
     7501 cttgggacgt tctgtggggg aggccgcggc agacggaaac ggtgagattt caggcggaac
     7561 ggaaccggtt gaacgggttc gaccgttcaa gtcccgcgtg gtcgagggtc tgcgggtttc
     7621 ggcggggcgt caacggctcg acgtgatcgc gcaatccatg gccgcatcgg actgggaccg
     7681 tctgccggcg agcccggcct tggacatgcg ggagaacggg caggcctatg agattctttt
     7741 ttcgctgccc tcgggtgtgg accgcgacaa cgtgcgggtt acttcatccg gaagcctgct
     7801 cacactcgcc ctgaccgatc ccggcaccgg aaaggtgctc atgcggcgca ttcgaatccc
     7861 gtgcgaggcg gaaccaaaca gccgcatcca cagtgttgtc tccaaccggg tgctgcacgt
     7921 tcggatttat ccgtgaccag gcgcgggccg cggggccttc ccgacggcac ctcaacagca
     7981 gcgccgatgc cgttgttttc gcttttttga cactccctca agcaaggtgg tacgatcttc
     8041 ctgctcaagc gaggaggctt tttgtgaaac ccattgtccg gttattcctt acgggacttt
     8101 tcgtcctgtc cggtcgggtg gccgcacaac aggcttcagc ggttgccgcg aacatggaag
     8161 acatgggcaa cccggttgtc aggcggaaca aggtgaagga gcggatgcgt gtcgagcgga
     8221 tgcggcgcga agaagctgag gcgttcgcgc gttccttcgg gattccggtg cgggggcagc
     8281 gtcccaatgg cggtgcatgg gaactggcgg cgatcgaagg ggggaagccg atataccgca
     8341 tgacgctgaa tgcgaacgcc gcgatttcca cgggagcgaa tattctgcgt ctcgctccct
     8401 acggtgcgga cggagccggg gatacggtcg gcgtatggga tgcctgttca gcgcggacca
     8461 cccacaggga attcggggga cgggtggttt cccgggacgg tgcgggtgtc gtgcacgatc
     8521 actcgacgca tgtggtcggc acgatctgtg ccgcggggat tgacagcttg gcgaagggaa
     8581 tggctccggc tgtgcttgtg gacagctatg attggaatga cgaccagggt gagatgacca
     8641 tgcgcggtgc ggcctatccc ggtgaagcgg acaagatcaa ggtttcgaat cacagttatg
     8701 ggatggtcgc gggatggggg tatagcggga atccgaaatt cacttggtac gggaccggat
     8761 tcacggcgtc cgcgatcgaa gacgattttg gcaaatataa cacgtatgcc cacgagatgg
     8821 acaagttggc tttcgacctg ccttattatc tggtcttctg ggcggccggg aatgatcgcg
     8881 gcccctcgga aaacccggtg aacggcgata cggtggcact cagcgccggc ggcatccccg
     8941 tcacctatga ttcgtccctg catccgcccg cagatcatgt ttatcgaaac gggtatgaca
     9001 cgcttggata cagtgcgctg gcgaaaaacg tcgtgacggt cggtgccgtc accgacgcgg
     9061 tcagtggggg catgcggagt gtcgccaacg cgaacatgac gattttttcc tcatggggac
     9121 cggctgacga tgggcgcatc aaaccggatc tggttgccaa cggggatcgt ttgaagtcaa
     9181 cttcgtatgg cggggattcc tcgtacagtt actcgagcgg gacgagcatg gcctgtccga
     9241 atgcggctgg cacggcgcaa caacttgtcc acctcttcgc gaatctcttc agtaatcagg
     9301 tcatgcgcgc gagtacgctc aaggcgttgc tgatccacac agcggacgat ctggggaatc
     9361 ccggtccgga ttatgtttac ggttggggac tgctcaacgg caaggccgtt gcggatctgc
     9421 tgctcgacta ccgtgcgcag gccggtaggc ggcaggtgat cgaaaaccgc gtgagtaccg
     9481 atcggaatgc aatcagtttt gaaatgattt gggatggggc aagtccggtc cgcgcgacgc
     9541 tttgctggac cgatccgccg ggtgtggcga ccgggatagg ggattcccgc cagccccgtc
     9601 tggtcaacaa tctcgacctg cgggtgatcg ctcccgacgg aaccgtccac gagccatggg
     9661 tcatgccgtt tgtgggggaa tggagcgtgg cctcctgcgc gtatcccgcg ataaccggtt
     9721 cgaacaccac ggataatgtc gagcaggttc tgatcgcggc gccggttgtc gaggggacgt
     9781 atacggtccg tgtcacgttt gcgggcactc tcacaaacgg acaacagccc ttttctctga
     9841 tcgtgagcgg aggcgtgccg gaacagcagg cgccgagacc ggttttgatt tcctcttcgc
     9901 cgggttcggg agagggggtt ttgccgttca ccttcacagg ggatcatttc ctgttggggg
     9961 ctacggcgcg attgcgccgg ttagggcaac cggatgccct ggcgatgaac atgcaggtcc
    10021 tgggggatgt ggcctatgcg cgcgtgaaca ccgtgggcat gtccagcgga tggtggaatc
    10081 tcacgctggc caatccggac ggcggacggg cgacgttgta taatgccttt gtcgtgccgg
    10141 aactgttctg gagtgaaaca ttcgaaacaa acgacctttt atcccgcggc tggtcgtttc
    10201 tgcagacgct ggggaccaat cagtgggagc tctcgacggt taagagcgtc tcgccgtcga
    10261 actccgcctt ttcgttgggt gtggacacac gatctgacac aagtctggtt tcccctccta
    10321 tcgagatccc gttggatcaa acggctttgc aactgagctt ctggcatgat ttctcgttta
    10381 gcgccaatga cgcgggggtg ctggagtttt cgctggataa tggcgattgg tatgacgtga
    10441 cggatatcgg ttcgggggtt gttttttccc agaacgggta taccgggact gtggcgggca
    10501 agggtgcgcc gaattcgctg aatccgctgg tagggcgttc tgcctgggtg ggcacaagcg
    10561 gagggtttgt cccggttacg ttgtccctga ccgatacggc acggtacgcg ggccgccagt
    10621 tgcgcgtgcg ctggcgtttg ggtacggaca acagcacggc cagtgaggga tggtatgtgg
    10681 atgatgtgcg cggattgggg gtctccgcgc cgccaccggt tcctcccgac ggcacggtgt
    10741 ttggagtccg ttgacccccc tccgagggcc gggcgaaaac gaggggcaac aggagtttca
    10801 tggtgtggat tcaggcgagg ccgcggtcga aattaccccg cagaacatga ctttgcggca
    10861 gcttcccggc catcacgttt cggatcgttc gctccgcatc aatggtttcc ggtgaccagg
    10921 aaaggtcgat acggaaacga cctacgcctg cggcgtggag ttccggaagc cggtcggcca
    10981 gacagtaggg ctcgtcggcg gtggttaccc atagacgatc ccggacgaga acggtgaagg
    11041 tcttgccgcg gcggttggcg atccggatgt cccgcgtccg ccctttatcg gggggcgggc
    11101 gtccgcccgt caggggggcg gtttcagaga gaaagagagg ggggtgcaga taaacgggca
    11161 tttcggcttc gggtcggtgc cccgcgcacg ccgggatgtg gcagagcgca cggatattgt
    11221 ccagtccgtt ctccggggaa agcacctgca ccgcgaaacc gagacgggcg tattccgcgc
    11281 aggcggcccg gttcagggcg tacaaactcc agtcggcgga gtacgggctg agtccttcct
    11341 ctgtcaacat gcgccagccc gagagatctg cgcactccca gcgctcccat ccctcgcgtg
    11401 caaggacacg gagggcgtcc cgcatttccc gtgtagcgca cgcgcgcgtg atgaccggca
    11461 gcgcggggca gatcgcggtc tgggggacgg cttcgcgcca cgcctccaac tgtcgcttca
    11521 gcgcatcggc gggggcgtgg ccgatccgca ggatcaaggt tgacaggccc gacagcgccg
    11581 cagtcggcag gggttgcgcg tcggcgcggt gtttcagggt ccaatgcggg agcggcgcgg
    11641 cgtcaggtga gggagacgga atggcgggct ccggccgcca cttccgcagg atcgcctctc
    11701 ggcgcgttgc ggcatgttgg tcccacgccc cgttgaggcg gacgagcacg tcgcgccggc
    11761 acgcgttcag ttgcgagggg ggcgcatagc gcccgtctgg atcgtggaaa gtgagggtgc
    11821 ccagacgcca cgcagagtca ccgaggcggc tgaaggcttt ctgaacggcg gccggacttt
    11881 gctcgggttg gcgcgaaggg gaaaggggca gggcgatttg cgtttcggct gtgacctgtc
    11941 cgcagggttc gcagggggtc gaggcccgga cggcaattcc cccgggatcg agtcgcacct
    12001 cgaaatccac cggacgccct gtggtgagac cagactcgcg cacactctgg attccgtagc
    12061 ggcggcggac ggcttgcgag gcggagcaga acaccggtgt gccgcgcacg atgggcggga
    12121 acgggccttc cggtagcgcc acttcgacgc gttcgcccgc ggccagcgag agggccggac
    12181 gccgcgttcc ggcctgccgc aggcgcataa ccgcaaaacc cgggggacgg ccctccccgg
    12241 ggaattcgag ctgcaagccg tcatgtttct ctagggcacg gtcggaacgg aaacgcagcc
    12301 accgtgtgcc gtcacggtcg cggatcacag cctcgacctc cccgatccgt gcgccgcggt
    12361 ggccgaccgt caacggatca atgatcgttt cgggttgcgc gtgtgggtct tgggcgtaga
    12421 gttgtgttcc ggagcggctg aagatcgtct ggagatcctg aagccgttcg cgttccgtct
    12481 cgggggagag ggtgcggtcc aacttacggc ggtagtaatc ggtgacgcag gccacataga
    12541 gcggactctt catgcggcct tcgatcttca gcgcgtgcgc gccgctgccc gtcacgtcgt
    12601 caagcagcgg cgcgaggcac aggtcgcgca tcgagaaggg gaaatgtggc gcggcgttgt
    12661 cggacgcgca ttccgctgcg gtgaagggtt cgcggcagca ataggcgcac cgtccgcggt
    12721 tgccgctccg cccggtggcg agagaggaaa agaggcagag accgctgtag ctgtaacaaa
    12781 gcgcgccgtg aatgaaaatc tcggtctgca tgccgctttc ttgcgaaatg cggcggattt
    12841 cgtcaagggt cagttcgcgc gccagcacca cccgcgtgaa cccgagttcc cggagtgcga
    12901 gcgctccctc cagattgtgc gtcgccaatt gcgtgctggc atggagggcc agccggggga
    12961 agtggtcgcg gatcagccgg gccacgccca ggtcctgcac gatcacgccg tccggcgtca
    13021 ggtcgtccaa gacggccagc aggtcgagtg ccggctccca ctccgactcg gtcaccagag
    13081 tgttgaaggt gacgtacacc cgtttggcgg gagtgaagct ctgcgcatag gccttcagca
    13141 ggcgcagccg gtccggggtg aggtgctctg cgtcggcgcg ggcggagaag cgcggcaagc
    13201 cgagatacac cgcgtccgcg ccgtattgga aggcggcgca cgcggcctcg aaaccgcctg
    13261 cgggagctag gagttcaggg gtcaaggcac gaacttcttg ttacaaaacc aacggcgtgg
    13321 tgcggaatgg atgatccatc ctttccgtcg cgatgccgac gtatgaatat gaaatagaag
    13381 accggctttt gcaagccggt cttgggggga tacataggag tacacatgga gaaacagcct
    13441 tgaaacaaca aactgttctc gaacagcaaa gaaatatgta gcagaaaaaa agagggaatg
    13501 caagtgcgat ttttagattt tttcccggaa ttggagcggg cccgatgcct ttgaaggttc
    13561 tgattatgga ggaaatcaag ttgacgaaag agctcttttt cggacagaat atgaaccgtc
    13621 acgaaacgag cggttatgaa acgttctgta tctataccga aaaggatttg aggcgaatga
    13681 ggaagactga tctctctggc gagtggaatc tctacaagcg cggctctaaa aaaagcattc
    13741 cggcacacgt gccggggtgt gtgcatgccg atttgatggc tgcgggcgag atggaggatc
    13801 tggtgcgcgg aacccggctt gaggcggcgc gttgggttat gggtgaggag tgggtatatg
    13861 aaaaaacctt tgtgcaggag gatgtttcgg cctatgaccg ggtggttttg tgtttcggcg
    13921 ggttgaacgg gtgtgcggcg atccgattga acgatgccga actgggtcag gtcaccaacc
    13981 cctttgaaac ggttgaattc gaggttaaac ccttgttgaa ggcggggaag aaccgtctgc
    14041 gggtaacctt ctcccccccg acgtcaaccg tgccgggggc ggaggagggg ccgaatcagg
    14101 atttcatctc cctgtcgccc accgtgggga tcgggcgcac ggtttcgctg ctggccttca
    14161 cgcatgtccg ggtcagcgag gtgcatatcc ggcaggactt tgcgacagcg ggcgtggtca
    14221 agctgcatgt ggcggtggcg tcagagcgat atgaccccgc gatgcatttg gagttgctgg
    14281 tgcgggtctg ctataagggc aacatcctgc atgaggcgcg cgacctgctt aaccgggacc
    14341 ggatgacact ctccctgacg gtcaagaacc cccagctttg gtggccggcc ggtctgggtg
    14401 agcaaccgct gtacgaagtg acggttgacg ttttgtcggg ccggacctgc tacgagcacg
    14461 tcgcgcggcg gatcgggctc cgtcaggtgg agatggaacc ggtccgcggc agggccttcg
    14521 gccgtgtgaa agtgaacggc cagtcggttt tcctcaaggg ggccagttgg gtgccggccg
    14581 acctgtatcc ggcgcgcttg acgcgggtgg agtatgcccg gctggtgaaa gccgtggcgg
    14641 tcgcgaacat gaactgtctg cgcgtctggg gtggggggct ttacgaaaac gatgcttttt
    14701 atgatctgtg cgatgagtac ggcatttgcg tgtggcagga tctgatgctc gggggcagcc
    14761 ggggcgcgcc gcccgcaaaa ggggaacggg cctcgttcga gcgggaagtg cggagcacgg
    14821 tcatggcgtt gcgccaccat ccgtgtctgg cgctttggtg cggcggggac ggtggcggga
    14881 aagcgatcga ttccgcgtac accaagatcg cggtcgacgg tgtgcgtgcg gcggatccgg
    14941 acacgccctg tctgccgccg atcccgcacc agccgttctc cctgacggaa ccggccgggt
    15001 acatgacgat gccttcgttc cccgagccgc gcgtgatcgc ggaatatctg gatgaggggg
    15061 agcggaatgc cggacatccg gcctgcgcgt ttcacatgtc gccttccgat ggcgcgcgcc
    15121 gcatctatca cgggttcctc gaccattttc tggttccctc cagtttcgat catgtcgctt
    15181 ggctcagcca gatccagcag gcggtgctgt tgcggcgggc ctatgagcgg gtgcggaccg
    15241 cgaaggaggc ttcacccgga tttctctatt ggcatttcaa tgactgctgg cccacgtgca
    15301 gcccgtccgc cgtggattac gcgggacggt ggaaagcgtt gcagtatctg gcccgccgtt
    15361 ttttcgcgcc gatctcgctg tgcggggttt actgccggga atcgggcacg gtcacgctga
    15421 atgtgtttca tgacgggctc cggccgacgt tcaagggtga gatgcagtgg cgggtgaccc
    15481 agatggaggg aatcgtggtt gccgagggca cgaaaaaggt gtcggtggcc ccgttgtccc
    15541 gtgatgaatc ggttcgcgtc aaagtggcag acatcctgaa acgctgtggc gcggacaacc
    15601 tgatcatgtg gatctatctg ctggatgagc agggcaatca ggtggcgtgg aatacggtgc
    15661 tgttctgcga gccgcgcgcg ctgaaactgc aggcaccgcg tatgcgggct gaaatccgtg
    15721 cctgggatga caacagcttc gccgtgacgc tcaccagtca ccatcctgcc ctgtgggtct
    15781 ggatttcgct cgacggcatg gacgcgcgga tggatgacaa ctttttctcg cttgaaccgg
    15841 acaagccatt ccgtgtccgc gttatcccgg ccacccggat gaagctcgac gccttccgcc
    15901 agatcctccg catcggttcc ctgcgcgaca cctggcagga caaacgcctg ttgatgcagg
    15961 tgatggccgc ggccaaaaag aaatagcgtg cattgcttct gggtttcagg tttctggttt
    16021 cagggtttct ggtttcaggg tttcatccaa atcggggtcg gggtcgggat cgctatcggg
    16081 atcgattatt ccaccacggg acactttctt gggtttcagg gtttcaggtt tcagggtttc
    16141 agggtttctg gtttcagggt ttcatccaaa tcggggtcgg g
//
LOCUS       DAOSLE010000060        16006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3821_length_16006_cov_8.702777, whole genome shotgun sequence.
ACCESSION   DAOSLE010000060 DAOSLE010000000
VERSION     DAOSLE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 16006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16006
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3821_length_16006_cov_8.702777"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..679
                     /locus_tag="PK576_05725"
     CDS             185..679
                     /locus_tag="PK576_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOE61128.1"
                     /translation="MLKLIRTSLSREAHPLVQFIKYGVVGGMATGVQILTFFLCGWFL
                     FPCLTQDDIVVRLLGLTAPAMSASVRAWNAGFCNAIGFLFSNTFCYVLNRLFVFKPGR
                     HHWVMEFLLFFAVSGISMVLGTAIQSFLITYQGIQTTFAFGANILCALLINYAMRKFV
                     IFKG"
     gene            complement(713..2743)
                     /gene="metG"
                     /locus_tag="PK576_05730"
     CDS             complement(713..2743)
                     /gene="metG"
                     /locus_tag="PK576_05730"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019570578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE61129.1"
                     /translation="MGNTKRKMVVTSALPYANGDIHIGHLVEYLQTDFWVRFQKMRGH
                     ACVYVCADDTHGTPIMVRARAEGISPEELIARSYERHTRDFADFEIQFDQYHSTHSEE
                     NRALCNTIFESMRQSGAVTVRKLPQLYCEQDAMFLPDRFVQGVCPKCGAAGQYGDACD
                     VCSQTYPAEELKEAHCTLCGNVPVTRDSEHLFFELEPFRDFLKAWIPDHTAPDVANKL
                     LEWFNEPLRGWCISRDAPYFGFEIPGYPGKFFYVWVDAPIGYMASLQKWCALHGESFD
                     AWWQQPGGELYHFIGKDIVRFHCLFWPAMLHTADYKTPDQVFVHGFLTVNGEKMSKSK
                     GTFIKARTWLEHLKPQDLRFYYACKLNGTTDDMDLNLGDFISRVNSDLVGKITNLASR
                     GAQMLHKSLGGRMGSMDVEGRALYEKAVGKAERIAAHYENRDFSKVMVEVRDLADQAN
                     QYFDGQAPWALVKTDPEAARAVMTSILNLFRVLTIYLQPVLPGYAARVAALFNEQPYV
                     WPDIARPVENLPIGPYVYLATRVEQASVERLIEASREPERSAVPAQSAKPAAQPAEPV
                     RETIDYDTFSQMDLRVAEVLSAEPVEGADKLLKLQLDIGEERPRQVFAGIRRHCDAAS
                     LVGCRVVMVANLAPRKMRFGVSEGMILAAGNPDGTLFVIQADPGALPGATVK"
     gene            complement(2953..4248)
                     /locus_tag="PK576_05735"
     CDS             complement(2953..4248)
                     /locus_tag="PK576_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE61130.1"
                     /translation="MTITEKILARESGASVVSPGDNIWVNVGILLTHDVCGPGTIGIF
                     HREFGKDAKVFDREKIVILPDHYIFTADEKAHRNVDTLRVFVKEQGLPYFYDPGTENY
                     CGVCHVAMPELGHVRPGETIFGTDSHTCTHGALGAFSTGIGNTDAGFIMGTGKLLVKV
                     PPTIRFVLDGELPPYLSAKDLILHIIGDISVDGATYHAMEFTGSAIEALSIDERCTIC
                     NMAVEAGAKNGIIAPDRKTIDYVKARTKKPFEPLFSDPDAKVADERRYDVSRLEPVVA
                     KPHLPDNKALARACSDVKIDQVYIGSCTGGKLEDFIMAAKVMQGKRVAVRTLLVPATR
                     QVEKGLDETKIGGQSLRAIFEEAGVEPIAPPSCAACLGGPVDTFGRTHGHEVVVSTTN
                     RNFIGRMGSKDARIYLASPYTAAASSLTGHITDPRTVRL"
     gene            complement(4245..4952)
                     /locus_tag="PK576_05740"
     CDS             complement(4245..4952)
                     /locus_tag="PK576_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61131.1"
                     /translation="MSKKPTQFDFWFAVNNTRIVVPPKRHLETFGNTIIRYHLVSELM
                     DTVGKVRVREGRMQALRPQIITPSSYSTMILEGFGEQAQRYVEWLKEHEDTVRILRYG
                     YTLKQEAFSEQVVSDATEAVLERVKAEAEAHNDPFAAVIHGVDDPWDVCLVRLFWTVI
                     HNSAQENIRELHERRLFEMQDGIPAGLREEIERGFQAAAQDAKLIKPLGRLLQEHGLF
                     ERYQDRFFSLVQGKGGT"
     gene            complement(5245..9015)
                     /locus_tag="PK576_05745"
     CDS             complement(5245..9015)
                     /locus_tag="PK576_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61132.1"
                     /translation="MKNRKFHMFSAVGLTGCLLFGAAGAALADTIYKADTATLNTSAD
                     WVGGTNASIEHIGRFDSLLSAANAASLSLGADLALGGLYFANNLKGPVSIAAGNTLTL
                     GASGISASSANQTVTFNHDIQLAADQTWRAANKALTLNGDLAGDAGITLIKTGKNDYN
                     NLWSLTASNTFAGTVDIREGHLRLYNTTGSLIWADVIVSTGAEFRVNGTAGIAPAEGL
                     RGKRVTLNGGLLRLTGPGTMPIHETFSGPLRIGPGFSEVLAIPDTNPLKPVRLTFQRL
                     ERESGGVVFLHGSNLGDQPFGVSDSRYANIEFIEPPTLVGGGGAYNTPNASIIPYAVC
                     NPYSSDPPYYFATYESGYGVRQLKSYASGILDGSVTTTNARLTTTATLNQSTTINALI
                     LEGEGSIEGPGTLTLTSGALMSAGNRSVNSIGINTPGTLAFGTAEAILNFRYRTNTLV
                     IGSAVTGSAGLTKGSAGNLSLLGPVDMGGVIDFANGRLTVGDTVNGHIRLSRDGTLEM
                     LSGSSISGAVSVSGGNVYLTNTCAISGALDIGESRTVTMSNGGSVTGRITVFSGGTLT
                     LNSGSVISGNLTNFGTFNYNSTDTEPITISLDGLGTVYQRNVNMPLTLFQSPGEHWVG
                     NIVPTEGATVILDGEPDATTVVNNMLPEPARTKWTIENGVWIFNPLHPSKNLSADVTL
                     NGGVLTVTPETRRFTFYTAGGTNTLTITGGVLMDQPETDWSLRFGNHHGSWEQTGCNF
                     IGVQTGGIVLVQSKEYLHLGSLADHTTATYDLSGGTVYAFGQSDNAVSIGSGTNNNSL
                     AAFTLRGSGKLVSRKRIYGNRAEEGANQVFAFQGGTLATASYEAVNLRSEPGGARGTL
                     FNEGGTLAPGDFGVPGRTLVNGSYTNAPNAVIALDIGGASQANSFTNGAGYHDHLMIT
                     GNAVLDGALRISLINGFLPDPASLFYAISCDNPMTFAAGGFSNAEDNQVWAEDGFARF
                     DVTLSNDSKHLLLGNAALNQWAGASGGDWDASGAWSLTNPATAEFGAYFGPNLTAAGT
                     VTAEAGRTLRGLAFNNAVSYTVAGPGALTLASDGAYGMPRILSLLGSHTLTAALVLQD
                     ALTVDVANAAGTLTLAGGVTGGKALTKLGAGTLALAGRNVLGAVTVAAGAAAQTGGVS
                     AFDALEVSPGGVFRLVSGTLTLAQGLTVAPGGAFDFGSYGGGTLRLAVGAPGGAVDTA
                     TEFWEAVADGRVTVKGQAAADPSLFILKEEQSGGVTYVTVTLKSFGTLLQFR"
     gene            9653..11026
                     /gene="dnaA"
                     /locus_tag="PK576_05750"
     CDS             9653..11026
                     /gene="dnaA"
                     /locus_tag="PK576_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE61133.1"
                     /translation="MNAAAELWANACNHLKSVLNADVFSRWIEIIRPATLEENCLSLE
                     VENDFSQAWLEDNYKGFILDALHLAGAPSELSVTFVVNAPEAQEGLPDDPPQETKPAP
                     RRRPKTPNRTSSNQLNPIFTFENFVTGPSNSFAHAAALGVSQSVGRAYNPLFIYGQTG
                     IGKTHLMQAVGHRVLSSPGMSVFYVTTETLLNEYVEAIQNRTTMEFRKKYRKTDVLLV
                     DDIQFLAGKDSLQAEFFHTFNVLYDAHKQIIMTSDLPPRELSGLEPRLVSRFEWGLVT
                     EIESPDFETRLAILRYKHSMSKVQLPDEMLVFIADNIRSNVRSLEGALTRAITFASLN
                     GTITLDSLRNLLKDQLDDERQKDLTFDDIQRMVADYYDLRMTDMSSKRRPRSVAAPRQ
                     IAMFLCRKLTRSSLPEIAKSFDKTHATVVHACKTIQDRIQTEEDLRDSVREITRKLGR
                     DPVSFQI"
     gene            11337..12431
                     /gene="dnaN"
                     /locus_tag="PK576_05755"
     CDS             11337..12431
                     /gene="dnaN"
                     /locus_tag="PK576_05755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020150035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE61134.1"
                     /translation="MKLTIMRSAFLDGLQTVQNVVSSKGVLQILSNALLQAKDGKLSI
                     TTTDLDIAERCEVECEVEREGSTTLPVRRLVGMIRELPEGKVLVEVNDQDEATVQSGS
                     SFYKIIGLPTRDYPPIPATEGKFCYRIDQGAFREMLRKTSYAVSQDETRRVLNGVLMA
                     FKENKLTLVATDGRRLALVEHEIEFPPEAETEMILPIKAVSELMRILSNDGDLKIYAQ
                     KNQAVFELGTTMLSSKLIDGVYPNYRQVIPAACDERVVIERDALLAALRRVSVVTTDK
                     CNATRLTFSANQLIVSINTPEVGEGRDVVPVKYGGKEISIIFNPEFVMDPLKNMDDDE
                     VFIEMNDGHSPALIKCGIPFLYVLMPLRIS"
     gene            complement(12399..13028)
                     /locus_tag="PK576_05760"
     CDS             complement(12399..13028)
                     /locus_tag="PK576_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61135.1"
                     /translation="MTYRYRRDAVRLPDAQLHHITLRLAFYESHVDGAALLFLTAREP
                     LTALRLDVRDLEIFEVAVSLAETLSPDDRERFAEPFPPTDGFSACAHTLDTVARKLVI
                     PLPRPLAPGASLRVYVRCRCVPSDALLEGLYRDVTPPGAPQQYISQCQHARAYRPAPP
                     PFRGRASGGLSGRGSVTWAEPSMKHRQDVRATAETKQKLLTDPQGHQHV"
     gene            complement(13068..13385)
                     /locus_tag="PK576_05765"
     CDS             complement(13068..13385)
                     /locus_tag="PK576_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61136.1"
                     /translation="MRRNTRLRIFGWLACAAVAHAQAPAKRPPPEQRGERSPPAVVTN
                     AAPHAAQTNAAAKARTPAKPTFTRKQVERRLRMLQELRDRGLILQPFYERKVRECEEG
                     LVE"
     gene            complement(13408..15735)
                     /locus_tag="PK576_05770"
     CDS             complement(13408..15735)
                     /locus_tag="PK576_05770"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOE61137.1"
                     /translation="MMRRGILWIVGLTGCAAASVLGAPGEVTLAERGKADGMAIVRSE
                     EASASQRHAAEELQRFTEQMTGVRLPIVTDREPLPARAVLLGVTRHTAAILGTAADAA
                     AFGDDGFRIVTRPPHLLILGGPVRGTLYGVYELLERVGGCRWYASWHSVIPRLERFTV
                     PNTDETQKPAFALREPFWFDMFDGDLAARNKANGNAMRLTERHGGKIRFGGGLFCHTF
                     NRLCPPEEFFETHPEYFSEIDGRRIKEPSQLCLTNPDVLKIVTTRVLERIRKDPTAKL
                     FSVSQNDWRYPCTCPACKAIDDREESHAGTLITFVNQVAEAVEREFPEVWIETLAYQY
                     TRKPPKTVRPRRNVVPRLCTIECDFSYPLDQSAYEQNRKFVEDIRGWSAMTDKLYIWD
                     YTTNFRHYTAPFPNVLALRDNVRFFRDHRVVGLFEQGAYHGRHGDFAELKAWLLAKWL
                     WNPDLPTEPLLADFFAGYYGAAAPLVRQYFDELHTFYNDPAKKPLRIFDDVRSPVLPD
                     AFLDRAADLWSRAEAAVRDSPAHAYNVRMGAFSVLYARLSRLPASEQKTVWVTSNPQQ
                     YAVPAEYRALATELLKRFDEARNIRLAESLERHTKTITDWRALISPPPLPPPGDRATI
                     EDEQLSLGRPGVWGEKKVADPQAGDGFALKLFNTHYEWCTSLPFGKVAFDPGARYRIR
                     IRLRVERVPGQEGTAFWAGVYDPVNKKGHGKTVEPKTSAIGDGYAWYDVAEWVPESAH
                     YVWIGPGRFDKKTQNANPAIRAVYIDCLELQRLEK"
BASE COUNT         3043 a         4981 c         4774 g         3208 t
ORIGIN      
        1 accctgaaac ccgaaaccca agaaagtgtc ccgtggtgaa gcaatcgatc ccgatcccga
       61 tagcgacccc gatttggatg aaactacgaa accttgaaac cagaaaccct gaaaccccga
      121 aaccccgaaa ccccgaaacc aaagagagtg gctccgttgc gacggttatg gtatgatccc
      181 gcccatgttg aaactgattc gaacgagtct gagccgcgaa gcccatccgc tggtgcagtt
      241 catcaagtac ggcgtggtgg gcggtatggc cacgggggtg cagatcctca ccttctttct
      301 gtgcggatgg tttctcttcc cctgtctgac ccaagacgac atcgtggtgc ggctgctcgg
      361 cctcacggcg ccagcgatgt ccgcgtccgt ccgcgcctgg aacgcggggt tctgcaacgc
      421 catcggcttt ctcttctcga acaccttctg ctatgtgctc aaccggttgt tcgtcttcaa
      481 gccgggccga caccattggg tgatggagtt cctgcttttc ttcgcggtct ccggcatcag
      541 catggtgctt ggtacggcga tccagtcctt tttgatcacg tatcagggga tccaaaccac
      601 cttcgcgttc ggcgcgaaca ttctctgtgc cctgctgatc aactacgcga tgcgcaaatt
      661 cgtgattttc aaagggtgat tgcgcaaagg ggcgtgcgtt gtgaagttct ccttacttca
      721 ctgtcgcgcc cggcaacgcg cccggatcgg cctggatcac aaacagcgtg ccatcgggat
      781 tgcccgccgc gagaatcatg ccctccgaca caccgaagcg catctttcgc ggcgcgagat
      841 tcgccaccat caccacacgg cagcccacca gcgaggcggc gtcgcagtgc cgacggatgc
      901 ccgcgaaaac ctgacgcgga cgctcctccc cgatgtcgag ctgcagcttg agcagcttgt
      961 ccgcgccttc caccggttcc gcgctgagaa cctcggccac acgcagatcc atctgggaaa
     1021 aggtgtcgta gtcgatcgtc tcccgcaccg gttcggcggg ttgcgcggcc ggttttgcgg
     1081 actgtgccgg aacagcggaa cgctccggct cgcggctggc ttcgatcagc cgctccacag
     1141 aggcctgctc cacccgcgtg gccagataca cgtaagggcc gatcggcaga ttctcaaccg
     1201 ggcgcgcgat gtccggccag acgtacggct gctcattgaa aagtgccgcc acccgggccg
     1261 cataccccgg cagcaccggc tggagataga tggtcagcac gcgaaacagg ttcaggatcg
     1321 atgtcatcac cgcgcgcgcg gcctcgggat cggttttgac cagagcccag ggtgcctgac
     1381 cgtcgaaata ctggttggcc tgatcggcaa ggtcgcgcac ctcgaccatc accttgctga
     1441 agtcgcggtt ctcatagtgc gcggcgatac gctcggcctt gcccacggct ttctcgtaca
     1501 gcgcgcggcc ctccacatcc atgcttccca tacggccgcc cagactcttg tgcagcatct
     1561 gcgcaccgcg cgaggcgagg ttggtgatct tgcccaccag atcggagttc acgcggctga
     1621 tgaaatcacc gaggttcaga tccatgtcat ccgtggtgcc gttcagtttg caggcgtagt
     1681 aaaaacgcag atcctgcggc ttgaggtgct cgagccaagt gcgcgccttg atgaaggtgc
     1741 ccttggattt gctcatcttc tcgccgttga ccgtgaggaa accgtgcaca aaaacctggt
     1801 ccggcgtctt gtagtcggcc gtgtgcaaca tcgcgggcca gaagagacag tggaagcgca
     1861 cgatgtcctt cccgatgaaa tggtacagct cgccccccgg ttgctgccac caggcgtcga
     1921 acgactcgcc atgcagggcg caccatttct ggagggaggc catgtacccg atgggcgcgt
     1981 ccacccacac gtagaagaac ttgcccggat acccgggaat ctcgaagccg aagtaggggg
     2041 catcgcgcga gatgcaccaa ccgcgcagcg gctcgttgaa ccactccagc agcttgttgg
     2101 ccacatccgg cgcggtgtgg tcagggatcc acgctttcag aaaatcgcgg aacggctcga
     2161 gttcgaagaa aaggtgctcg ctgtcgcgcg tcaccggcac gttgccgcac aaggtgcaat
     2221 gggcctcctt cagctcttcg gcgggatagg tctgactaca gacatcgcag gcgtcgccgt
     2281 actgtccggc cgcgccgcac ttcggacaga cgccctgcac aaaacggtcc ggcagaaaca
     2341 tcgcgtcctg ttcgcagtag agctgcggca gtttgcgtac cgtcacggca ccgctctggc
     2401 gcatcgactc gaaaatcgta ttgcagagag cgcggttttc ctcgctgtgg gtggagtggt
     2461 actggtcgaa ctggatctcg aaatccgcga aatcgcgggt gtgccgctcg tagctgcgtg
     2521 cgatcagctc ctccggggag atcccctccg cacgcgcgcg caccatgatc ggtgtgccat
     2581 gcgtatcgtc cgcgcagaca taaacgcaag cgtggccgcg catcttttga aaccggaccc
     2641 agaaatccgt ctgcagatac tcgaccaaat gcccaatgtg aatgtccccg ttggcgtacg
     2701 gaagagccga cgtcaccacc atcttgcgtt ttgtgttgcc cataaagtgc tcccacggta
     2761 aaaaaacgaa gtgttatcat agcgtgtccg ccccattccc gccacttctt agttcatcag
     2821 tgccttacga accaacgagc acaataaaaa caagttgttt caccctcgtt ctttgagatt
     2881 gcgggctccg cccgcgttgg gggttgaggc tgcaagattt gtacagcctc acagtctcac
     2941 aacctcacaa cctcacagcc tcacagttct cgggtcagtg atgtggcctg tgagggatga
     3001 ggccgccgcg gtgtagggcg aggcgagata aatgcgcgcg tcctttgagc ccatgcgtcc
     3061 gatgaagttg cggttggtcg tgctgaccac cacctcgtgt ccatgggtac gcccaaaggt
     3121 gtcgaccggc ccgcccaggc aggccgcgca ggagggcggc gcgatcggct cgacgcccgc
     3181 ctcttcaaag atcgcccgca gactctgtcc gccgatcttg gtttcatcaa gccccttctc
     3241 cacctgccgt gtggcaggca caagcagcgt gcggacggcg acccgcttgc cctgcatgac
     3301 tttggccgcc atgatgaaat cctccagctt gccgccggtg caggaaccga tgtagacctg
     3361 atcgattttc acgtcgctac aggcgcgagc cagcgctttg ttgtcgggaa gatgcggctt
     3421 ggccacaacc ggctcaagcc gggagacgtc gtagcggcgc tcgtccgcaa ctttggcgtc
     3481 cggatcgctg aacaaaggct cgaacggctt cttcgtgcgg gccttaacgt aatcgatggt
     3541 cttccggtcg ggcgcgatga tgccgttctt cgcgccggcc tcgaccgcca tgttgcaaat
     3601 cgtgcagcgc tcatcgatgc tgagggcttc aatcgcggaa cctgtgaatt ccatcgcgtg
     3661 gtaggtcgcg ccgtccacac tgatgtcacc gatgatgtgg aggatcagat ccttggctga
     3721 gaggtacggg ggcaattcgc cgtccaacac gaaacggatg gtcggcggca ccttgaccaa
     3781 cagcttaccc gtgcccatga taaaccccgc atcggtgttg ccgatgcccg tcgaaaaggc
     3841 tcccagcgcc ccgtgcgtgc aggtatggga atccgtgccg aaaatggtct cgcccggacg
     3901 cacatggccc agttccggca tcgccacatg acaaactccg cagtaatttt cggtgccggg
     3961 gtcatagaaa taaggcagcc cttgctcttt gacgaacacg cgcagcgtgt ccacgttgcg
     4021 gtgggctttt tcgtccgccg tgaagatgta gtgatccggc agaatcacga tcttctcgcg
     4081 atcaaaaacc ttggcgtctt taccgaattc gcggtggaaa atgccgatcg tgccgggtcc
     4141 gcacacgtca tgcgtcagga ggatcccgac gttcacccaa atgttgtcgc cgggcgacac
     4201 cacgctcgcg cccgactcgc gggccagaat tttttccgtg atggtcatgt tccgcctttc
     4261 ccctgaacca gactgaagaa gcggtcctga taccgctcaa agagcccgtg ctcttgcagc
     4321 agtcggccca gcggtttgat cagcttcgcg tcctgcgccg cggcttggaa cccgcgttcg
     4381 atctcttcgc gcagaccggc cgggatgcca tcctgcattt cgaacagacg ccgctcgtgc
     4441 aattcccgaa tgttctcctg agccgaattg tggatcacgg tccagaaaag ccgcaccaga
     4501 cagacatccc acggatcgtc caccccgtgg atcaccgccg caaacgggtc gttgtgcgct
     4561 tcggcctcgg ccttcacacg ctccaacacc gcctccgtcg catccgaaac cacctgttcg
     4621 ctgaaggcct cctgcttgag cgtgtaaccg taccgcagaa tgcgcaccgt gtcctcatgc
     4681 tccttcagcc actcgacata acgctgcgcc tgttccccga acccctccag aatcatggtg
     4741 gagtaagagg aaggcgtgat gatctgcggc cgcaacgcct gcatgcggcc ctcccggacc
     4801 cgcaccttgc cgaccgtgtc catcagctcg ctcaccaaat gataacggat gatcgtgttg
     4861 ccgaacgttt ccaaatgacg cttgggcggc accacgatcc gcgtgttgtt caccgcgaac
     4921 cagaaatcga attgtgtggg tttcttactc ataacatgag aagggagtat aacaggaata
     4981 ccggattctg aaaagcgcga gaaaagaccc ggttcccgcg cgttgagtga ccggccccgc
     5041 ccgtaacggc ggatcaaacg gaccggtcgg acacacgcgt tcttgccgat cgtcgcgggt
     5101 caacgttcag cattgccgtc acttcgggcg tccgagacgt ccgcccgcac tttaccgccg
     5161 gagcggacgg ttcgcgaaac catcctcacg aggacgagat ggcgatcccg atcccgacag
     5221 cgatttcgat aaacccggaa cccattacct gaactggagc agcgtgccga agctcttgag
     5281 ggtgaccgtg acataggtca ccccgccgga ctgctcctcc ttgaggatga agagcgaggg
     5341 gtcggccgcg gcctggccct tgaccgtgac ccggccgtcc gccaccgcct cccagaactc
     5401 cgttgccgtg tcgaccgcgc cgcccggagc gccgacagcg aggcgcagcg tgccgccgcc
     5461 gtagctgccg aagtcgaacg cgccgcccgg agcgaccgtc aggccctgcg cgagggtcag
     5521 ggtcccggag acgaggcgga agaccccgcc cggcgagacc tccagcgcgt cgaaggccga
     5581 gacgccgccg gtctgcgccg ccgcgcccgc cgcgaccgtc accgcgccca agacgttccg
     5641 ccccgcgagc gccagcgtgc ccgcgccgag cttcgtcaga gccttgccgc ccgtgacgcc
     5701 gcccgcgagg gtcagggtcc cggccgcgtt cgccacgtcc acggtcagcg cgtcctgcag
     5761 gaccagcgcc gcggtcaggg tatggctgcc cagcagcgag aggatgcgcg gcatgccgta
     5821 cgcgccgtcc gaggccagcg tcagcgcgcc cggccccgcc accgtgtagg agaccgcgtt
     5881 gttgaaggcc agcccgcgca gcgtgcggcc ggcctcggcc gtcacggtcc cggcggcggt
     5941 caggttcggg ccgaagtacg cgccgaactc cgccgtggcg gggttggtca ggctccaggc
     6001 cccggacgcg tcccagtcgc cgcccgaagc cccggcccac tggttcagcg cggcattgcc
     6061 cagcaggagg tgcttgctgt cgttggagag ggtcacgtca aagcgcgcga aaccgtcctc
     6121 agcccagacc tgattgtcct ccgcgttgga gaagcctccc gccgcgaatg tcatcggatt
     6181 atcgcaactg atcgcgtaga agagcgacgc cggatcggga aggaagccgt tgatcagcga
     6241 aatccgcagc gcgccgtcca atacggcgtt cccggtgatc atcaggtgat cgtggtaccc
     6301 cgcaccattc gtgaaggaat tggcctggga cgcgccgccg atatcgaggg cgatcaccgc
     6361 gttcggcgcg ttggtgtagc tgccgttcac gagggtccgg cccggaacgc cgaagtcgcc
     6421 cggcgcgagc gtgccgccct cgttgaacag cgtcccgcgc gcgccgcccg gctccgagcg
     6481 caggttgacc gcctcatacg aggccgtcgc cagcgtgccg ccctggaagg cgaagacctg
     6541 attggccccc tcttcagcac ggttcccata aatgcgctta cggctcacga gtttgccaga
     6601 acccctcagc gtgaaggcgg cgaggctgtt gttgttcgtt cccgagccga tcgagaccgc
     6661 gttatcgctc tgcccgaacg catacaccgt gccgccagat aggtcgtagg tggccgtcgt
     6721 gtgatccgcc agacttccga ggtgcagata ctctttgctc tgcacaagca cgatgccgcc
     6781 cgtctgcacg ccgatgaaat tgcaccccgt ctgttcccag gaaccatggt gattgccgaa
     6841 gcgcaagctc caatccgtct ccggctgatc catcagcacc ccccccgtaa tggtcagcgt
     6901 gttggtgccg cccgcagtgt aaaaggtgaa cctgcgcgtc tccggcgtga cggtcaggac
     6961 gccaccgttg agcgtaacgt cggcgctcag gttcttagac ggatgcaacg ggttgaagat
     7021 ccagactccg ttctcgatcg tccactttgt ccgcgccggt tcgggaagca tgttgttcac
     7081 cacggtcgtc gcgtcaggct cgccgtccaa aatgacggtc gcgccctctg tcgggacgat
     7141 gttgccgacc cagtgctcac cggggctttg gaaaagcgtg agcggcatat tgacgttacg
     7201 ctggtagacg gtgccgagcc cgtcgagcga gatcgtgatg ggctcggtat ccgtgctgtt
     7261 gtagttgaac gtgccgaaat tggtcagatt ccccgagatg accgagccgc tgttgagcgt
     7321 cagggtgccg ccggaaaaaa cggtaatcct gcccgtcacg ctgccgccat tcgacatcgt
     7381 caccgtcctg ctctcgccga tgtccaatgc gccggagatg gcgcaggtgt tggtcagata
     7441 gacgttaccg ccggacacgg agaccgcccc ggatatcgag ctgccggaga gcatctcgag
     7501 agtcccgtcg cgggaaaggc ggatgtggcc gttcacggtg tcaccgaccg tcagccgacc
     7561 gttggcgaaa tcaatgactc ctcccatatc cacggggccg aggagcgaaa ggtttcctgc
     7621 agaacccttg gtcagccccg ccgaaccggt cacggcactg ccgatgacca gcgtgttggt
     7681 acggtagcgg aaattgagaa tcgcctcggc agtgccgaac gcgagggttc ccggcgtgtt
     7741 gatgccgatg ctgtttacgc ttcggttccc ggctgacatc agggcaccgc tggtcagggt
     7801 cagggtaccg gggccttcga tgctgccctc cccttctaag atcagggcgt tgatcgtggt
     7861 agactggttg agcgtcgccg tggtggtgag acgggcgttc gtggtggtga cgctgccgtc
     7921 cagaatgccg gaggcgtaag atttgagctg acgaacgccg tagcccgact cgtacgtcgc
     7981 gaagtaatac ggcggatcgg agctgtaggg gttgcacacc gcatacggga tgatgctcgc
     8041 gttgggagtg ttgtaggcgc cgccgccgcc gaccagcgtg ggcggttcga tgaattcgat
     8101 gttggcgtaa cggctgtcag acacgccgaa cggctgatcg ccgagattac tgccgtgcag
     8161 gaacacgaca cccccggatt cacgctccag ccgctgaaac gtcaacctga ccggcttgag
     8221 cggattcgta tccggaatcg cgagcacttc cgaaaagccg gggccgatgc gcagcggtcc
     8281 gctgaaggtc tcgtggatgg gcatcgtgcc gggtccggtc aggcgcagca agcccccgtt
     8341 gagcgtcaca cgtttccccc gcagcccctc ggccggggcg atgccggccg tcccgttgac
     8401 gcggaactcc gcgccggtgc tgacgatgac atcggcccaa atcagcgacc ccgtggtgtt
     8461 gtagagccgg agatgccctt cgcggatatc cactgttccg gcaaacgtgt tcgaggcggt
     8521 caggctccag aggttgttat aatcgttctt cccggtcttg atcaatgtga tccccgcatc
     8581 gcccgcaagg tcgccgttga gtgtcaacgc tttgttcgct gcacgccaag tctgatcagc
     8641 cgcgagttgg atgtcgtggt tgaaggtgac ggtctggttg gcggaggagg cgctgatacc
     8701 cgaggcaccg agggtcaggg tattcccggc ggcaatcgac accggccctt tcaggttgtt
     8761 cgcgaaatac agccccccca acgcgaggtc cgcgcccaac gaaaggctcg cggcgttggc
     8821 cgcgctgagc aaactatcga accggccgat gtgctcgatc gaggcgttcg tgccgccgac
     8881 ccagtcggcg gaggtgttca gggtcgcggt gtcggcctta taaatcgtat cggccagcgc
     8941 ggcaccggca gccccgaaga ggagacaacc cgtcaacccg acagcggaaa acatatggaa
     9001 tttcctgttt ttcatcgtct ttcacgctcc ttcttttcgc cactctttgt cagcaagccc
     9061 tgtccccaac gatggtaatc gtaaccccaa ccaaggcagg aagtcaaccc cgctgacgtt
     9121 taacctaaaa agagcagttg aacctccaac tctctttggt ttcaaggttt cgtggtttca
     9181 gggtttcagg gtttcaaggt ttcagggttt catccaaatc ggggtcggga tcggtatcgg
     9241 gatcggtatc gtcgacccgc gacccgcgac attcgacccg cgacgatcga cagggacgcc
     9301 cgctggtagg gacggttccc cgaaccgtcc gcggacgcct cagtcccgca tacgcattcg
     9361 gttaaaaaaa tcatattaat cctgtcatcc tgatgccctg aacggggggc ttgttgataa
     9421 ttttaacccg gggtctgatc atattgggga tatcgggggt ttttttgcct ttaactgttc
     9481 ttttcagaag aagttacatt atctacagca ccctgttgat aacctgttgg ccgattgatc
     9541 atcttttccc cttgtcccct ctcttcaatt gtggttcaac agtacacgtt gcgggggact
     9601 gttccaatgc ctccttttac ctctttttcc gacctgtttt gtgagtgccg ttatgaatgc
     9661 tgccgcagaa ttgtgggcta acgcctgcaa tcacctgaaa tccgtactca acgccgatgt
     9721 cttctcccgc tggatcgaga tcatccgtcc cgcgacgctg gaggaaaact gtctgtccct
     9781 ggaagttgag aatgattttt cccaagcttg gctggaggat aattacaaag ggttcatctt
     9841 ggacgccctc catttggcgg gcgcgccgtc cgaactttcc gtgacctttg tggtgaatgc
     9901 cccggaagcg caggaagggt tgccggatga tccgccgcag gaaaccaaac ccgcgccgcg
     9961 ccgacgtcca aaaaccccga accggacctc ttccaaccag ctcaacccga ttttcacctt
    10021 tgaaaatttc gtgaccggcc cgtcgaacag tttcgcgcac gccgccgcat tgggtgtgtc
    10081 gcaatcggtc ggccgcgctt acaatcccct cttcatctac ggccaaaccg gtatcggcaa
    10141 aacgcatctg atgcaggcgg tgggacaccg agtcctctcc tcacccggta tgagcgtttt
    10201 ttacgtcacg accgaaacgc ttctcaatga atatgtggag gccatccaaa accggaccac
    10261 catggagttc cggaaaaaat accgcaaaac cgatgtgctg ctggtggatg atatccagtt
    10321 tctggctggt aaggacagtc tgcaggcgga gttttttcac accttcaatg ttctgtatga
    10381 cgcccataag cagatcatca tgaccagcga tctgcctccg cgtgaactct caggccttga
    10441 gccgcggctg gtctcgcgct ttgaatgggg tttagtcacg gaaatcgagt cgcctgattt
    10501 cgaaacccgt ctggcgattc tccgctataa gcactcaatg tccaaggttc aactaccgga
    10561 tgagatgctg gtgtttatcg ccgataacat ccgttcaaat gtccgcagcc ttgagggtgc
    10621 gctcacccga gcgatcactt tcgcctccct aaatggcacc ataacgcttg attcgctccg
    10681 caacctgctg aaagaccaac tcgatgacga acgccagaag gatctcacct ttgacgacat
    10741 ccagcgcatg gtagccgatt actatgatct gcgcatgacc gacatgtcga gcaagcggcg
    10801 cccgcgttcg gtggccgcgc cgcgtcaaat cgccatgttc ctgtgccgca aactgacccg
    10861 ctcttccctt cccgagatcg ccaaatcttt cgacaaaacc cacgccaccg tagtccacgc
    10921 ctgcaagacg attcaggacc gcatccagac tgaagaggac ttacgcgatt cggtgcgcga
    10981 gatcacacgc aaactgggcc gcgatcccgt ttctttccaa atatgaccgc ctcttgataa
    11041 cctgttgatt tccttatgat ttgcttctca catctcttca taacccggca gttcttaaca
    11101 gccccctccc cctgtacacc ttttcttcta cctttatcga agggttatca acatgatttc
    11161 actcctgttt tgtttttcat aacttattca tataaagcag gttgtagagt tatccacaag
    11221 gttcttgcct ctctactatt aggactgttc tttctttact ttaatacccg taatagacta
    11281 caatcgcacc ttttatgggt cattaacgca atgacctggc catgggagag aaaacgatga
    11341 aattgacgat tatgcggtcc gcttttcttg acggtttgca gacagtgcag aatgtggtga
    11401 gttccaaagg ggtactgcag attttgtcga atgcgctctt gcaggcgaaa gatggaaaac
    11461 tgagcatcac cacaaccgat ctggatattg cggagcggtg cgaggtggag tgcgaggtgg
    11521 agcgcgaggg aagcaccacg cttccggttc ggcgtctggt cggcatgatc cgcgaattgc
    11581 cggagggcaa ggtgctggtc gaagtgaatg atcaggatga ggccacagtg cagagcggat
    11641 cctctttcta taagatcatc gggctgccca cacgggacta tccgccgatt ccggcgacgg
    11701 aagggaagtt ctgctaccga atcgatcagg gcgcgttccg cgagatgctg cgcaaaacca
    11761 gttacgccgt gtcgcaggat gagacccgcc gtgtgctcaa cggcgtgctg atggccttca
    11821 aagagaataa attgacgctg gtcgccacgg atggacgccg gttggctctg gtcgaacatg
    11881 agattgaatt cccgcccgag gccgagacgg aaatgatcct gccgatcaag gcggtcagcg
    11941 aattgatgcg cattctctcc aacgacgggg atttgaaaat ctacgcgcag aagaatcaag
    12001 cggttttcga gttggggacg acgatgctca gcagcaaact catcgacggc gtgtatccca
    12061 actaccggca ggtgattcct gcggcgtgcg atgagcgcgt cgtgattgag cgcgatgcgt
    12121 tgctggcagc gctgcgccgc gtttcggtcg tgaccacgga taaatgtaac gccacgcgat
    12181 tgacgttctc cgcgaaccag ttgatcgtca gcatcaacac gccggaagtc ggcgaagggc
    12241 gcgatgtggt tccggtcaaa tacggcggca aggagatctc gatcatcttt aatcccgaat
    12301 tcgtcatgga tcccctcaaa aatatggacg atgacgaagt ctttatcgag atgaatgacg
    12361 gtcacagccc cgcgttgatc aagtgcggca tcccgttcct atacgtgttg atgcccctgc
    12421 ggatcagtta ggagtttttg cttcgtttcg gctgtggcac ggacatcttg cctgtgtttc
    12481 atggacggtt ccgcccatgt cacggaaccc cgtccagata agccgccaga cgccctgcca
    12541 cggaagggtg gcggagcggg tcgatacgct ctcgcatgtt gacactgcga aatgtactgt
    12601 tgcggcgcgc cgggcggcgt gacgtcccgg tagagtccct cgagcagcgc gtcagaggga
    12661 acacagcggc agcgtacgta aacccgcagc gacgcaccgg gggccagcgg tcgtggcagc
    12721 gggatcacca gcttgcgggc caccgtgtca agagtgtggg cacacgcgga aaagccgtcg
    12781 gttggcggaa acggctccgc aaaccgttcg cggtcatcgg gggaaagggt ctctgccaac
    12841 gaaaccgcaa cctcgaaaat ctccaggtca cgcacatcga gtcgcaacgc cgtcaacggc
    12901 tcgcgcgcgg tcagaaacag gagggccgcg ccatccacat ggctctcgta aaacgcgagc
    12961 cggagggtga tatgatggag ttgcgcgtca ggcaggcgca ccgcgtcgcg tcggtatcgg
    13021 taggtcatgt tttgtttcgc tcagaaaagc cggatgttac ctttgcgcta ttccaccagc
    13081 ccctcttcgc actcgcgcac tttgcgctca tagaagggtt gcaggatcag gccgcggtcg
    13141 cgcagctctt gcagcatacg gagacgccgc tccacctgtt tgcgggtgaa ggtgggtttg
    13201 gccggggtcc gcgcctttgc cgcggcgttt gtctgcgcgg cgtgcggcgc cgcgttggtg
    13261 accaccgccg ggggagaccg ttccccgcgc tgttcgggag ggggcctctt ggccggggct
    13321 tgggcgtgcg ccaccgctgc gcacgccagc catccgaaaa ttcggagccg tgtgttgcgt
    13381 ctcattgccg gatcctcccg cgtccggtca cttttccaag cgctgaagct caaggcagtc
    13441 gatatagacc gcacggatcg cggggttcgc gttttgggtc ttcttgtcga accgtcccgg
    13501 cccgatccaa acgtagtggg cgctctccgg cacccactcg gccacatcgt accaggcgta
    13561 tccgtcgcct atggcggagg tcttgggctc gaccgtcttc ccgtggcctt tcttgttcac
    13621 ggggtcatac acgccggccc agaaggccgt cccctcctgc cccggcaccc gctcgacacg
    13681 caagcgtatc cggatgcggt accgcgcgcc ggggtcgaag gccaccttgc cgaacggcag
    13741 cgaggtgcac cattcgtagt gggtgttgaa caacttgagc gcgaagccgt ccccggcctg
    13801 cggatcagcc acttttttct cgccccacac gcccggccgg ccgagcgaca gctgttcgtc
    13861 ttcgatggtc gcccggtctc ccggaggcgg caggggtggc ggcgaaatga gcgcccgcca
    13921 gtccgtgatg gttttcgtat gccgctccaa actctccgcg agacggatgt tccgggcctc
    13981 gtcgaaacgt ttcagcagtt cggtcgccaa ggcgcggtat tccgccggga ccgcatattg
    14041 ctgcggattc gaggtgaccc acacggtttt ctgttcgctt gcgggaagtc gggagagccg
    14101 tgcgtacaac accgaaaagg cccccatgcg cacattgtag gcgtgggccg gcgaatcccg
    14161 caccgctgcc tctgcccggg accacaagtc cgcggcgcgg tcgagaaacg cgtccggcag
    14221 gacggggctc cgcacgtcgt caaagatccg caggggcttt ttcgccggat cgttgtaaaa
    14281 ggtgtgcagt tcgtcgaagt actgccgcac gagcggcgcg gccgcgccgt aatatcccgc
    14341 gaagaaatcc gcgagcagcg gctctgtcgg cagatcggga ttccacagcc atttggcgag
    14401 gagccaggcc ttgagttccg cgaaatcgcc gtgccgtcca tgataggccc cctgctcgaa
    14461 cagtccgacc acgcggtggt cgcggaagaa ccgcacgttg tcccgcaacg ccagcacatt
    14521 ggggaacggg gcggtgtagt ggcggaaatt ggtggtgtag tcccagatgt aaagtttgtc
    14581 ggtcatggcg ctccagccgc ggatatcctc gacaaacttc cggttctgtt cgtaggcgct
    14641 ctgatccagc ggatacgaga agtcgcactc gatggtgcag aggcgaggca cgacattgcg
    14701 gcgcggccgc acggtcttgg gcggtttgcg cgtgtactgg tacgcgagcg tttcgatcca
    14761 cacctccggg aactcccgct ccaccgcctc ggccacctga ttgacgaagg tgatcagcgt
    14821 tccggcgtgc gactcttcac ggtcgtcgat cgccttgcac gcgggacacg tgcacgggta
    14881 gcgccagtcg ttctggctca cgctgaaaag cttggccgtg gggtctttgc ggatgcgctc
    14941 cagcacgcgg gtggtgacga tcttcagcac gtcggggttc gtcaggcaga gctgggaggg
    15001 ctctttgatg cgccgaccgt cgatctcgct gaaatattcc gggtgggttt cgaaaaactc
    15061 ctcgggcgga cagagccggt tgaaggtatg gcagaagagc ccgccgccga agcggatctt
    15121 gccgccgtgc cgttcggtga ggcgcatggc gttgccgttg gccttgttcc gggccgcgag
    15181 gtcgccgtca aacatgtcga accagaaggg ctcgcgcaac gcgaaggcgg gtttctgcgt
    15241 ctcatccgta ttcggcacgg tgaaacgttc gagccggggg atcacgctgt gccaggaggc
    15301 gtaccagcgg cagccgccaa cccgctccaa cagttcatac acgccgtaca gcgtgccgcg
    15361 gaccggaccg ccgagaatca gcagatgcgg cggacgggtc acgatgcgga acccgtcgtc
    15421 gccgaacgcg gccgcatcgg cggcagtgcc gagaatcgcg gcggtgtggc gcgtgacgcc
    15481 cagcagcacc gcgcgcgccg gcaacggttc ccggtccgtc acgatcggca gacgcacacc
    15541 ggtcatctgt tcggtgaacc gctgaagctc ttccgcggcg tggcgttgcg acgccgacgc
    15601 ctcctcggac cggacgatcg ccatgccgtc cgcctttccg cgctcggcga gcgtcacctc
    15661 gcccggcgcc cccagtacgc ttgccgccgc acatcccgtc aacccgacga tccaaaggat
    15721 tcctctgcgc atcatgtcac ccccctttcg ttctttacgc cccctgtttt acccatgtgc
    15781 ccgatcaaca aggcaccgcc tctcctccgc ccgcgttccc tgatctcctg aaaagtcgtg
    15841 gcagggcgtt cgggcgcgga acagttgtcc tttcacgcag gagtataccg gttcggccgc
    15901 ttccggacaa taccccctgc cgcttcagcg taatcggtta gcgacaggag tgtgcgaaga
    15961 caatgcgttt ctggtgaaaa gggggggcag tgagtcggga gcgcgg
//
LOCUS       DAOSLE010000061        15901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3854_length_15901_cov_8.895242, whole genome shotgun sequence.
ACCESSION   DAOSLE010000061 DAOSLE010000000
VERSION     DAOSLE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3854_length_15901_cov_8.895242"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1612)
                     /locus_tag="PK576_05775"
     CDS             complement(347..1612)
                     /locus_tag="PK576_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61138.1"
                     /translation="MNAHHDQPGNVALDTHVADLTITSAERDVLRKLGERVMSLSRRQ
                     IEAEKKVLWTRQNQLQPSRPLILCDPEFSWREIIPDTSLQCEHALARHWERRLRKEIF
                     WGEKMGDDRVVESRFTVGWVYRRTFWGPDASSIHPQSTWSDLNDLSGLQFQTITVDRD
                     ATTDVVHLAREVFDRVLPVKLREQWYWSLGLTRELIKLRGFEQFMRDIRENPAGVHNL
                     MAFLRDGTLDLITFLEQAGLYSLNNEGDYIGSGGFGWTTQLPAKDYQGTARAKDLWAL
                     FESQDTRDLSPELFDEFVLQYQKPVMERFGLVCYGCCEPISERWHLLQNVPNIRRVSV
                     SPTCNRAKMAEALQDRYVYSCRPNPAILAASSFDADAARKEIRDILEKAKGCHLEIIM
                     KDHDTIHNDPARAIAWCQIAREEIARYYQ"
     gene            1968..3596
                     /gene="aroB"
                     /locus_tag="PK576_05780"
     CDS             1968..3596
                     /gene="aroB"
                     /locus_tag="PK576_05780"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE61139.1"
                     /translation="MHLFLYGPPGSGKSTIGRLLAQRLDLPFTDLDAVIEASAKQRIR
                     DIFACEGEAGFRTRETRALAETIAAPPAVIALGGGALLKGENRAMAEAAGEILCFDAD
                     AATLERRVACAPGVRPLLNVPSSDCATPPAPGQGKPIATLLSERSAHYASFPLRVKVS
                     DRPPEETADAAQTALGLFRVSGMGSAYRVRVGNGLLDTLGACFRELDCGDRTVIVGDT
                     HTLPLYGERAAAPLRALGVTVSLVPIPAGEDSKTIDTVAALWSAFLKAGIERGHAVIA
                     LGGGVVGDLTGFAAATWLRGVRWIGLPTTLLAMVDSSLGGKTGADLPEGKNLIGAFHP
                     PALVLADTATLATLPGRDLRCGLAEAVKHAVIGDPGLLDTLPRFAFCKDGDASIPCAR
                     LSDAAWLAPFVARAMAVKIRIIREDPFEKGFRAALNLGHTIGHGIEKATHFAVRHGEA
                     IAIGTVLEARLAEELGLAQPGLAGKLANLFSSLGLPVVPPPDIDPAEVLAAITLDKKK
                     TDGKVRFALPVGLGEVRTGVTVDDASLTRVLKRG"
     gene            complement(4048..5697)
                     /locus_tag="PK576_05785"
     CDS             complement(4048..5697)
                     /locus_tag="PK576_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOE61140.1"
                     /translation="MRRQFLPWLALMCVLAGALLPVWGWDVEHDEVAMLVGETLPAEI
                     RSFFSFSDFGVLIAFCHYPDMTEWPRENGKRGYHSLEEIKERVGAEDAAILAQQGYTN
                     SGWLHRERARATVMGLLARAFAKGEHSRAAFYISVLSHSVSDESALNHPPLLHFVQYS
                     RFPGVDYALRKVEPGSKNVFGFRSDGPVVRRVREKLHNDVPRVPQGSFEAAVFGFVTD
                     AVRQAGYAAEKEGKIAFAPRLEAEDALAELVAMQVRILVDMTATCWAHKAVDAVLPDA
                     GFDAACRKEMDRLFSKLDPAKQAVFDGIFDAALNPAQPKGVVGLVCEPYGMFSRPGLS
                     YVGRMITASAGRTLRDHGYAVKGIATRDLAKGLPPPTEMGAVMISLGQSGHLAPDEAD
                     ALKRYRDAGGRLFVVGGSDPSDISGMKDFLEMRADDEVPVSRKWGVQNQDVWSNMVAV
                     VDGVRTPLRLNPNIDGFCKPYASYAFKPDADVVPLAELDNGRTRFVVAARKGNVVWIP
                     EYLLMPFIFSGDTTANWAALRLDSFATRFFMSLLPRIARKE"
     gene            complement(5844..6416)
                     /locus_tag="PK576_05790"
     CDS             complement(5844..6416)
                     /locus_tag="PK576_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE61141.1"
                     /translation="MGKVGSKEKTRRFLLANIGRVIESHELQEAADGAVQYSRRLREL
                     RDEEGWPILSHHDSSELKPGQYLLSGEPPRKERPHFARTISAKLRAEVLDRNGFTCQM
                     CGLTPGEIDPATNRPVRLHVGHIKDKSLGGKDELSNLRTLCSTCNQGAKNITAEKPST
                     IWLLSQVRRAGQDEQKAVYEWLKRKFEKKP"
     gene            complement(6435..7442)
                     /locus_tag="PK576_05795"
     CDS             complement(6435..7442)
                     /locus_tag="PK576_05795"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007468506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOE61142.1"
                     /translation="MGRFTVAEFCVGGGGQALGLEAAGFDCVAAVEVDADCCTTLRLN
                     RPQWNVLHEDMRQVDGSRWRDTDLFAAGVPCPPFSIAGKQLGTGDERDLFPDAIRIIR
                     EMKPKAVMLENVPGFASAKFSEYRDNLRMQLLDMGYESDWRILQAADYGVPQLRPRFI
                     LVALPSDRMSFFEWPQPLSEQTTVGDTLQDLMAQRGWKGAQKWVKNARKIAPTIVGGS
                     KKHGGPDLGPTRARKQWRELGVDGLGIANEPPGADFPKEAFPRLTVRMVARIQGFPDF
                     WKFSGRKTAAYRQVGNAFPPPVAASVGLAIANAFDGKYPVRQPRKAIQLKLLEQTVPY
                     C"
     gene            7645..7800
                     /locus_tag="PK576_05800"
     CDS             7645..7800
                     /locus_tag="PK576_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61143.1"
                     /translation="MQKRTVKTGTEPQWRVPATERATLNASASNLLDDDFQLYPGQEP
                     APSLTYA"
     gene            complement(7852..9717)
                     /locus_tag="PK576_05805"
     CDS             complement(7852..9717)
                     /locus_tag="PK576_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61144.1"
                     /translation="MSVLLAAALAMTLEPRTYAPEEIKVEKGGTPDAPLVIKAAERGK
                     SVISGAIAPKGWQTWRGEILVADLPLDPGAGELCLYQDGERMDNARWPNEGYAQIDDC
                     LGENTRATHDGGGKAWLDGIAVFDSPRLAAWAKEPEPKVFGLWFYLWAEQRSRLLAVD
                     PQKKTFTVEMIGEAKKWGMRPKANFFVYNMLSEIDRPGEWAIERKAGKVYFWPKKDVQ
                     KFPPVFAGGKTLVLKNLGNVVFDGLVFEYFTDSAVALDRTDNVEIRSCLFRHTGGWAI
                     WSANASKTRVVGCDMYDLGEGGVLLDGGDRMKLEKGDNVVDNCHIHDYGKLRPNYRPA
                     VQLNGCGNRASHNLIYNGDHQALAFRGNDHLLEYNVIHDMCRFNYDAGCIYACTRDWS
                     HRGTVIRHNLIYMTGMRPQTSATDAIYLDDWTSGVTCYGNIINRCTRGIHMGGGQDNH
                     FYRNLIINADLSVCLYSRGADGGFSAKTANMGKDSFIYKRLTDHLDVYGSEKWKAAYP
                     KMLDLLKHDPVDAHLQFDNVVTGNVFVACGSMLLPGAKARETCVTEPNETRTGDPGFA
                     DYGNFDWRIDGFEFEKMGLYDDPRRFSPAMKFGPNVYRPAPPFRGSRLDGKENSF"
     gene            complement(9765..11510)
                     /locus_tag="PK576_05810"
     CDS             complement(9765..11510)
                     /locus_tag="PK576_05810"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HOE61145.1"
                     /translation="MMIRTMTVCAAALGLSAAAVGEQVDNRVRTLIHPVRVVATFPKA
                     GENYGDPAKVIAPEALLAGAPQQKNCGGVWKTSGCQLVNSGVAPGIVLDFGREIHGGL
                     QIMNCSQHGMKVRVRFGESVGETLVDIGDRGASNDHAIRDGVYSVPHMGAMEIGNTGF
                     RFVRIDLVTAGKLALDCVRAVSLMRPMKRLGAFKSSDERLNRIWETAVQTVHLCCQDQ
                     LWDGIKRDRLVWMGDTHPESMAILNVFGADAVLPETLDYAMHITDPDEKWMMNMAPYT
                     LWWLRNVYEWYFFTGDAEYVHQRGDYIRRTVRHCLSTMKDGTWECPRRVFLDWPSQHD
                     LVASKEGLQGLAAITFENSAEFAKILGDAALEADCRAAVAALRKRGGDARGCKQAAAM
                     LALSDLAEPKRMFDEVLGKNGHEGVSTFYGYYMLEAMSKSGANQRALDTIRDFWGAML
                     DVGATSFWENFSLAWTNNCFRIDELPIAGKKDIHGDYGEFCYPGFRHSLCHGWSCGPA
                     SWCIQHVLGIRVLEPGCRSIEIKPFLGDLDWAEGAMALPDGGCIKVRVEKRTDGRLKT
                     DVECDMEHVKWRVRD"
     gene            complement(11535..13181)
                     /locus_tag="PK576_05815"
     CDS             complement(11535..13181)
                     /locus_tag="PK576_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61146.1"
                     /translation="MFADALLLATLQIFSQVPVEDFNAANQARYLKDIRDAGITTVLV
                     SYSDYYGIGDSRKPYFAKIGREVRYFEEAGLDVRVWCNGFGYGNDRRVGAGKTRFANS
                     QNIVSMHGKTSGALCPLDPAMRRLLIENMRDIAATGVKAILMDDDFIQSARGFVGCAC
                     DRHLARVAKKVGHPVTHADMVRAFTGKPNALRTAFVDVTGEVAVELARELRAAVDEVN
                     PQTRIGLCLSVTHWDLEGVDMDELLRAFAGETRPFVRISGAPYWDDARFRGIGLEGVT
                     EFARMQAQWLQGKGYEIFDENDPYPRKVKVVPAWRCELYDKATIADGLMARHKYMLCY
                     GKDRTEPGYIEAHLANMKDDRKLAELFEGAEPYGLRIDFPKRRIREATLPAEPLEERR
                     ITELYSQPLAANLLLRCGIPIRHDDATASYVFRPEDFGIDVAAVDFAAFKGGDLRKRI
                     LEDVRRKSGRPLDVYVETASPRIYQIVRYNAKRSEYAVLLENMGTKSAEVKIVTAGTP
                     RITGSVCGKFATGPGGVTLADLPAHEFAAVRFALNDETGK"
     gene            complement(13171..15564)
                     /locus_tag="PK576_05820"
     CDS             complement(13171..15564)
                     /locus_tag="PK576_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOE61147.1"
                     /translation="MRRQATLLWTALSVGLFMDAAAAENGLVAHFTFDNPTQFGYDNV
                     QQAVIGTITSDSISGATPADPTSCDAPIVRGMQIATGWWQNNYMTVPGASFGSAQGIP
                     YGNQAVTYSLWIKPSPNWQTSGTLGMGTGCYLLRHCTAGHEWYKTYDHECLYIIRDGS
                     GFPTITFSVGEYNAAAASATYALGAAFDGGWHLITATYENRVLKLYWDGVKRAETTIA
                     ANVGVPNNSPLMFGHAAVDYNTYVQRRFAGAVDDIKVYNRALDADEILAAYNAGATAF
                     DTDVIVWKGASAGGAAEDGDNWSTKSNRRTVGEVYTAGAVFDVTAVTDDGTITHNADT
                     TLKLKGVICTNGQDGVTIRMQAGDIEMKRPSTQRGLVAHFAFDDPDNMYCDLSTNAFT
                     ATPGAGVAYGSANPVPLSGCAGITGNGLNFVNGWWLFDYLTVSGSCFNAAHGIPYGNQ
                     AVSYSFWIKPTPTWRDYQHLGLGHNGVYLLRHGENLCQWMDNRGHSFWIFKDGDNPKI
                     TWSAANGGAAAARTAAYTSTELFDGAWHQIAGTYEDKVLKLYFDGELKATTTVPDNLT
                     VANNSSLIVGADGLTNMDHSVQRTYAGGFDELKVFNVALTDAEVAAEYAQRSRNLDKV
                     PAGTVPSPAKVQLDAGTSVAVRGYGHQYGSLTGAGSVDVGYTSALTLDGTFAVAGALT
                     GGGEISAANLVLGGNASGFTGDFTLGENATVTITDSSDTFLNSTFGGKVTLPKKAVIA
                     YGGKRPNGGMATLTAATLGTPSDFTEWVDSTGGTVKVRAVDDTLQIQVIGGLVLYVR"
BASE COUNT         3173 a         5228 c         4390 g         3110 t
ORIGIN      
        1 gtcgcgggtc gaatgtcgcg ggtcgcgggt cgacgttccc gatcccgatc ccgatcccga
       61 ccccgacccc gatttggatg aaaccctgaa accctgaaac cctgaaacca gaaaccctga
      121 aacccagaaa ccctgaaacc ctgaaacctt gaaaccagaa acccagaaac cctgaaacca
      181 gaaaccctga aaccctgaaa cccagaaacc ctgaaaccag aaaccctgaa acccagaaac
      241 cctgaaaccc tgaaaccttg aaaccagaaa cccagaaacc ctgaaaccag aaaccctgaa
      301 acccagaaac ccaagagcgt tgaaggttca actgctcttt ttaggttcac tgatagtatc
      361 gggcgatttc ctctcgggcg atctggcacc acgcgatcgc gcgggccgga tcattgtgaa
      421 tcgtatcgtg gtccttcatg atgatttcga ggtggcaacc cttggccttc tcgagaatat
      481 cacggatctc cttgcgcgcc gcatccgcat caaacgagct ggcagcgaga atcgcggggt
      541 tgggcctgca agagtacacg tagcgatcct gcagcgcttc cgccatcttc gcccggttac
      601 aggtcggcga cacgctgacc cgccggatgt tcggcacgtt ttgcaacaaa tgccaccgct
      661 cgctgatcgg ctcgcagcac ccataacaca ccagcccgaa gcgctccatg accggctttt
      721 ggtattgcag cacgaactcg tcaaacagtt cgggtgacag atcccgggtg tcctgacttt
      781 cgaacagcgc ccagagatct ttggcacgtg ccgtcccctg ataatccttg gccggcagtt
      841 gcgtggtcca gccaaatccg cccgaaccga tgtaatcccc ttcgttattg agactgtaca
      901 gcccggcctg ttcgaggaag gtgatgaggt ccagcgtgcc gtcacgcaag aaagccatca
      961 gattgtgcac tccagccgga ttttcgcgga tatcccgcat gaactgctcg aaaccgcgca
     1021 gtttgatcaa ctcgcgggtc aggccaaggg accaatacca ttgctcgcgc agcttcaccg
     1081 gcaggacacg gtcgaaaacc tcacgcgcca gatgcacaac gtcggtcgtg gcatcccggt
     1141 caacagtgat ggtttggaac tgaagcccgc tcaaatcatt caaatcgctc catgtcgatt
     1201 gcggatggat cgatgaggca tcaggccccc aaaacgtgcg gcgataaacc catccgaccg
     1261 tgaaccggct ctcgacgacc cggtcatccc ccatcttctc gccccagaaa atctccttgc
     1321 gcaagcggcg ctcccaatgc cgggcgagcg cgtgttcgca ctgcaatgac gtgtccggaa
     1381 tgatctcacg ccaactgaat tccggatcac acaggatcag cggccgcgag ggttgcaact
     1441 gattctgccg cgtccaaagg acctttttct ccgcctcaat ctgccgcctg gacaacgaca
     1501 tgacccgctc ccccagcttg cgcaacacat cgcgttccgc cgatgtgatg gtgaggtccg
     1561 cgacatgcgt gtccagtgcg acattgcccg gttgatcatg atgggcattc ataaccgaac
     1621 cctccttttc ttacgacacc cgattccgat cagatcgccc cgggcggcat ggtgtcgtga
     1681 cggtccgccg aagcatttgt ttccgcacac ccacactaaa aggggcatgt tgaatgaccc
     1741 gaagtataaa ggcgcaaacc ggaattgtcc agcggggaat cggtggccgg agaaggacga
     1801 ttccgaaaaa tcccccgagc catgccctca aaaaccttcg tgtgagaaac aacttgtttt
     1861 tcaacggcag gccacataag atgcgcggtg ttcgatccgg caggggggga agccttcccc
     1921 tccggacgga aacacgaata cggtccaccc ctcacccacg gcatatcatg cacctttttc
     1981 tgtacggccc cccgggaagc ggtaagtcca ccatcggacg cctgttggcg cagcgcctgg
     2041 acctgccctt cacagatctc gacgcagtca tcgaagcctc cgccaaacaa cgtatccgcg
     2101 acattttcgc ctgtgagggc gaggccgggt tccgtacccg cgaaacacgc gcactcgccg
     2161 aaacgatcgc agccccccct gctgtgatcg ccttgggagg cggtgccctg ttgaaagggg
     2221 agaaccgggc gatggcggaa gccgcgggtg agatactctg cttcgatgcc gatgccgcca
     2281 cactggagag acgtgtcgcg tgcgcccccg gtgtgcggcc gcttttgaac gtcccgtcat
     2341 ccgactgcgc gaccccgccc gcaccgggac aaggcaaacc catcgcaaca ttactgtccg
     2401 aacgttcggc ccattacgcc tcttttcccc tgcgcgtgaa ggtgtctgac cggccgcccg
     2461 aagaaacggc ggatgcggcg caaaccgcgc ttggcctctt tcgcgtatca ggcatgggtt
     2521 ccgcctaccg tgtccgcgtg ggcaacggtc tgctcgacac cctaggcgcg tgtttccgcg
     2581 aactcgactg cggcgaccgt accgtgatcg ttggcgacac ccacaccttg ccgctctacg
     2641 gcgagcgtgc cgccgccccg ctccgcgcac tcggcgtcac ggtctcgctt gttccgattc
     2701 ccgcaggcga ggactccaaa accatcgaca ccgtcgccgc gctctggtct gctttcctca
     2761 aggccggtat cgagcgcggt catgccgtga tcgcgctcgg cggcggtgtg gtcggcgatc
     2821 tcaccgggtt cgccgccgcc acctggttgc gcggcgtgcg ctggatcggc ctgcccacca
     2881 cgctgctcgc catggtcgac tccagcctcg gcggcaagac cggcgccgac ctgcccgaag
     2941 gcaaaaacct gatcggtgcg ttccacccgc ccgcgctggt gttggccgac accgccacac
     3001 tcgccacctt gccgggccgc gatttgcgct gcggcctggc tgaagccgtc aaacacgcgg
     3061 tcatcggcga ccccggcctg ctcgataccc tgccacgttt cgccttctgc aaagacggag
     3121 atgcctccat accctgcgcc aggctctcgg acgctgcctg gctcgcgccg ttcgtcgccc
     3181 gcgccatggc cgtcaagatc cgcatcatcc gggaagaccc ctttgaaaaa gggttccgcg
     3241 cagcgctcaa cctcggacac acgatcggtc acggcatcga gaaagcaacg catttcgccg
     3301 tgcggcatgg tgaggccatc gccatcggaa ccgtgctcga agcccgtctg gccgaggaac
     3361 tcggcctcgc tcaacccggg ctggccggga aactcgccaa cctcttctcc agccttggtc
     3421 tccccgtcgt tccgcctccc gatatcgacc ctgccgaagt gctggcagcg ataaccctgg
     3481 acaagaaaaa gacagacggc aaagtgcgct tcgccctgcc cgtcggcctc ggcgaagtcc
     3541 gcaccggcgt aaccgtcgat gacgcgtccc tcacacgcgt gctgaaacgc ggctgaccgc
     3601 ctgatataaa caccccacac gcctgttggc aaggtaatcg gttcgtgaca ggggtgtgcg
     3661 aaggtcatgc gtttcagggt tccattgcgg cgccatcgct atcgccatcg ggatcggggc
     3721 ggttgactcg cgacccgcga cgagcgacct gcggtgtcgg aaacgcgggc gtccctgtcg
     3781 atcgtcgcgg gtcgaatgtc gcgggtcgac gttcccgatc ccgatagcga tcccgatccc
     3841 gaccccgatt tggatgcaac catgaaaccc tgaaaccccg aaaccctgaa acccagaaac
     3901 cccgaaaccc tgaaaccaga aacccagaaa ccagaaaccc cgaaaccccg aaacccgaaa
     3961 ccctgaaacc ctgaaaccct gaaacccgaa accctgaaac caaagagagt tgaaggttca
     4021 actgctcttt ttaggattac ctcttgccta ttccttccgc gcaatgcgcg gaagtagcga
     4081 catgaagaaa cgggtggcga aagaatcgag acgcaaggcg gcccagttcg cagtcgtgtc
     4141 gcctgaaaag atgaacggca tcaacagata ttcggggatc cacacgacgt tgccttttcg
     4201 cgccgccaca acgaaccgcg tgcgcccatt gtcaagttcc gcgagcggca cgacgtccgc
     4261 gtccggtttg aacgcatacg acgcatacgg cttgcagaag ccgtcgatat tcggattgag
     4321 ccgcagcgga gtccgcacgc cgtccacgac cgcgaccatg ttcgaccaga cgtcctggtt
     4381 ttggacgccc cacttcctcg aaacaggcac ttcgtcgtct gcccgcattt ccagaaaatc
     4441 cttcatgccg gaaatatccg acggatctga acctcccacc acgaaaagtc gtccgcccgc
     4501 atcacggtag cgtttgagcg catcggcttc gtcaggtgcc aggtgtccgc tctgcccgag
     4561 ggagatcatc accgctccca tttccgtcgg cggcggcagc cccttcgcaa ggtcacgcgt
     4621 tgcgattccc ttcacggcat aaccgtggtc gcgcaaggtt cgtcctgcgg acgccgtgat
     4681 catgcgcccc acataggaga gtcccgggcg tgagaacatg ccgtagggtt cgcacacgag
     4741 cccgaccacg cccttgggct gcgccggatt gagcgccgca tcgaagatgc catcaaagac
     4801 agcctgtttc gcgggatcaa gtttagagaa gaggcggtcc atctccttgc ggcaggccgc
     4861 gtcaaacccc gcatccggaa gaaccgcatc aacggccttg tgggcccagc aggtggccgt
     4921 catgtccaca aggatgcgca cctgcatcgc cacaagttct gccagggcat cttcggcctc
     4981 cagacgcggc gcaaaggcga tcttgccctc cttttcggcc gcgtaaccgg cctgccgcac
     5041 agcgtccgtc acgaaaccga acacagccgc ctcaaatgat ccttgcggga cacgcggcac
     5101 atcgttatgc agtttttcgc gcacgcgccg caccacggga ccatccgacc ggaatccgaa
     5161 cacgttcttc gagcctggct cgaccttccg cagcgcataa tccacgccgg ggaaacgcga
     5221 atactgcaca aaatgcagaa gcggcggatg gttgagagcg ctttcatccg aaacggaatg
     5281 tgacaaaacg gaaatataga atgcggcccg cgaatgctcg ccctttgcga acgcccgcgc
     5341 gaggagcccc atcaccgtcg cacgcgcacg ctcccgatgc agccagcccg agttcgtata
     5401 cccctgttgc gccagaatcg ccgcatcctc ggcgccgacc cgctccttga tctcttccag
     5461 tgagtgatag ccccttttac cgttctccct cggccactcc gtcatgtccg gatagtggca
     5521 gaacgcaatc agcacgccaa aatcggaaaa ggaaaagaac gaccgaatct cggcgggcag
     5581 ggtctcgccc acaagcatcg ccacctcgtc atgctccacg tcccatcccc atacaggcag
     5641 caacgccccg gccaagacac acatcagcgc caaccaaggt aaaaactgtc gtctcattct
     5701 ctttctccat cagttgacac ccattatgcg gggaggggta aaagagtcaa cccccaagct
     5761 gtaatcggtt cgcccgccgc ccgcgcttcg cacgggaatg taaatgagga taactttgat
     5821 cggtgtaagt gataggcaag gatttagggc tttttctcaa atttgcgctt tagccactca
     5881 tacacagcct tttgttcatc ttggccggcg cggcgcactt gcgaaagcaa ccatattgtg
     5941 ctcggtttct ccgccgttat gttcttcgct ccctgattgc atgttgaaca aagcgtacga
     6001 aggttcgaca actcgtcttt gccgccgagc gatttatcct tgatgtgccc gacgtgcaac
     6061 cgtaccggtc tgttggtcgc cggatcaatc tcgcccggtg tcaatccgca catttggcat
     6121 gtgaacccgt tacgatccaa cacttcggcg cggagctttg cggaaatcgt acgggcaaaa
     6181 tggggtcgtt ctttacgagg cggttccccg ctgagaagat attgccccgg tttcaattct
     6241 gagctgtcgt gatgtgatag aatcggccag ccttcttcgt cacgaagttc tctcaaccgc
     6301 ctgctgtatt gaaccgctcc atccgccgct tcctgaagct catgcgattc gataacgcga
     6361 ccgatgtttg ctaacaagaa ccgtctggtt ttctcttttg aaccgacttt gcccatgttc
     6421 ccctcatttt tatctcaaca atatggaact gtctgctcca acagtttcag ctgaatggct
     6481 ttgcgtggct gtcgaaccgg atatttgcca tcaaaagcat tggcaatcgc caatccgaca
     6541 gatgcggcga cggggggcgg gaaggcattg ccgacttgac ggtatgccgc agtctttcgc
     6601 ccgctgaact tccaaaaatc aggaaaccct tgtattctcg caaccatgcg aacagtcagg
     6661 cgggggaagg cctctttcgg aaaatcggcg ccggggggct cgttggctat gcccaggcca
     6721 tcaacaccca attcgcgcca ctgtttccgg gcgcgcgtgg gtcccaagtc ggggccgccg
     6781 tgcttctttg agccgccgac gatggtgggc gcgatcttcc gtgcgttctt tacccatttt
     6841 tgagcgcctt tccatcccct ttgcgccatc aagtcctgaa gcgtatcgcc aacggttgtt
     6901 tgctcgctga gcggttgcgg ccactcaaaa aaagacatcc tgtccgaagg gagcgcgaca
     6961 agaataaacc gaggccgcag ttgaggaacc ccatagtcgg ctgcctgcaa aatccgccaa
     7021 tcggactcgt agcccatgtc caaaagctgc atgcgcaagt tgtcgcgata ttcagagaat
     7081 tttgccgagg cgaatccggg gacgttttcc aacatgacag ccttgggctt catctcgcgg
     7141 ataatcctga ttgcatctgg gaacaagtcc cgctcgtctc cggtgccgag ttgtttgccg
     7201 gcaatggaaa agggggggca gggaacgccg gcggcaaaca aatcggtgtc gcgccatcgc
     7261 gagccgtcaa cctgtcgcat gtcttcgtga agcacgttcc actgcgggcg attgaggcgc
     7321 agcgtcgtgc agcagtccgc atcaacctcg actgcggcaa cacagtcgaa cccagcggcc
     7381 tcaagcccaa gagcctgacc gccgccgcca acgcaaaact ctgccactgt gaacctgccc
     7441 atggtgccgg tacttatacc atccttttcg ccggaaacgc aagtcccgcg ccgtaatcgg
     7501 tcggcaacga agggacaggt gtttagacgg ctttcgtgtg taaaaccggt tccgatgcgg
     7561 atattgaacg tcccgctata tgtaatggat gggtcttgtc ctatttcctg ttgggggcgg
     7621 agcaaaaacg gacacaattg caaaatgcaa aagcggactg tcaagaccgg caccgaaccg
     7681 cagtggcgcg tgcccgccac cgagcgcgcc accctcaacg cgagtgccag caatctgctg
     7741 gacgacgact tccagctcta ccccggccaa gaacccgccc cctctcttac ttatgcgtga
     7801 tgggagcgtg gctggttgaa cgctggaagt tgactgtgga atgttacgca ctcagaagga
     7861 gttctccttg ccatcaagcc gcgaaccgcg gaacggcgga gcggggcggt agacgtttgg
     7921 gccgaacttc atcgccggcg agaaccgccg cggatcgtcg tacagcccca tcttctcgaa
     7981 ttcgaacccg tcaatgcgcc agtcgaagtt cccatagtcc gcgaatccgg gatcacccgt
     8041 acgcgtctcg ttcggttcgg tgacacaggt ttcacgtgcc ttcgcgccgg gcaggagcat
     8101 ggagccgcag gcgacgaaca cgttgccggt cacgacgttg tcgaattgaa gatgcgcatc
     8161 gaccggatca tgcttgagaa gatcgagcat cttcggatac gccgccttcc acttttccga
     8221 accatagacg tcaagatgat ctgtgaggcg tttgtagatg aaactgtcct tccccatgtt
     8281 cgcggtcttg gctgagaaac cgccgtctgc cccgcggctg tagaggcaga cggaaagatc
     8341 cgcattgatg atgagattgc ggtagaaatg gttgtcctgt ccgccgccca tatggatgcc
     8401 gcgcgtgcag cggttgatga tgttgccata gcacgtgacg ccgctcgtcc agtcatcgag
     8461 gtagatcgcg tccgtggcgg aggtctgcgg acgcatcccg gtcatgtaga tgagattgtg
     8521 ccggatcacc gtgccgcggt gcgaccagtc gcgcgtgcag gcgtagatgc aaccggcatc
     8581 gtaattgaat ctgcacatgt cgtggatgac gttgtattcg agcaggtggt cgttaccccg
     8641 gaaggcgagc gcctgatggt caccgttgta gatgagattg tgtgaggcgc ggttgccgca
     8701 gccattgagc tgcaccgccg gacggtagtt cggcctcaac tttccgtagt cgtggatgtg
     8761 gcagttgtcg acgacgttgt cccccttttc aagtttcatc cggtctccgc catcgagcag
     8821 gacgccgccc tcgccgagat cgtacatatc gcagccgacg acgcgtgtct ttgacgcgtt
     8881 tgcgctccaa atcgcccagc ctcccgtgtg gcggaacaga cacgagcgga tttcgacatt
     8941 gtccgtacga tccaacgcca ccgcgctgtc agtgaaatac tcgaagacga gcccatcgaa
     9001 gacgacgttc cccagattct tcaaaaccag cgtcttcccg ccggcgaaaa cgggcgggaa
     9061 cttctggaca tccttcttcg gccagaaata caccttcccc gccttccgct cgatggccca
     9121 ctcgccgggc cggtcgatct cggagagcat gttgtagacg aagaagttag ccttcggacg
     9181 cattccccac ttctttgcct cgccgatcat ctcaactgta aaggtcttct tctgcggatc
     9241 gaccgcgagc agccgcgaac gctgctctgc ccagagatag aaccagagtc cgaacacctt
     9301 cggttccggc tccttcgccc aggcggcgag acggggcgaa tcgaagacgg cgatgccgtc
     9361 cagccacgcc ttgccgccgc cgtcgtgcgt cgcccgcgtg ttttctccca gacagtcgtc
     9421 gatctgcgcg tacccctcgt tcggccaacg cgcattatcc atccgctcgc cgtcttgata
     9481 gaggcaaagc tcgcccgccc ccggatccag cggcagatcc gcaaccagaa tctcaccgcg
     9541 ccacgtctgc cagcccttcg gcgcgatcgc gcccgaaatc acagactttc cgcgctccgc
     9601 cgccttgatc acgagcggag catccggcgt ccctcccttt tcgaccttga tttcctcagg
     9661 cgcatacgtc cttggctcca acgtcatcgc cagcgccgcc gcaagtagaa cactcataaa
     9721 acatgcctcc cctggtttcc ttgccttttc ctaaatcccg gttcctaatc cctcacccgc
     9781 catttcacat gttccatgtc acactccaca tccgtcttga gcctaccatc cgtacgcttc
     9841 tccacgcgca ccttgatgca tccgccatcc gggagcgcca tcgcgccctc cgcccagtcg
     9901 agatcaccga ggaacggctt gatctcgatc gaccggcagc cgggctccag cacccggatg
     9961 ccgagcacat gttgaatgca ccacgaagcg ggtccgcagc tccagccgtg acagagcgaa
    10021 tgacggaacc cgggatagca gaactcgccg tagtcgccgt ggatgtcctt cttcccggcg
    10081 attggaagct cgtcgatgcg gaagcagttg ttggtccagg ccagactgaa attctcccag
    10141 aagctcgtcg ctcccacatc gagcatcgcg ccccagaagt cgcgtatcgt gtcgagtgcg
    10201 cgctgattcg ctccggactt gctcattgcc tcgagcatgt agtagccgta gaacgtggag
    10261 acgccctcgt gaccgttctt tccgagaact tcgtcgaaca tccgcttcgg ctccgcaagg
    10321 tccgacagcg ccagcatcgc cgccgcctgt ttgcatccgc gcgcatcgcc accgcgtttc
    10381 cggagcgccg cgaccgccgc gcggcaatcc gcctcgagcg ccgcgtcgcc gagaatcttc
    10441 gcaaactctg cgctattctc gaaggtgatc gccgccagcc cctggagccc ctccttcgac
    10501 gcgacgagat catgctgcga cggccagtcg aggaagacac gcctcggaca ctcccaggtt
    10561 ccgtccttca tcgtcgagag gcagtgtcgc accgttctgc ggatatagtc gccgcgttgg
    10621 tggacatatt ccgcgtcgcc ggtgaagaag taccactcgt agacattccg cagccaccag
    10681 agcgtgtacg gcgccatgtt catcatccat ttctcgtccg gatcggtgat gtgcattgcg
    10741 tagtcgagtg tctcaggcag aaccgcgtcc gcgccgaaga cgttgaggat cgccatcgac
    10801 tctgggtgcg tgtcgcccat ccagacaagc cggtcccgct tgatgccgtc ccataactga
    10861 tcctggcaac agagatgcac cgtctgcacg gcggtctccc atattcggtt caggcgttcg
    10921 tcggacgact tgaacgcgcc cagccgcttc atcggccgca tcagcgatac cgcccggacg
    10981 cagtccagcg ccaacttccc ggctgtcacc agatcgattc gcacgaaccg gaacccggtg
    11041 ttgccgatct ccatcgcgcc catgtgcggc acagaataga caccgtcacg gatcgcatgg
    11101 tcgtttgacg cgcccctgtc cccgatgtcc accagcgtct cgccgaccga ttcgccgaaa
    11161 cggacgcgca ccttcatgcc gtgctggctg cagttcatga tctggagccc gccgtggatc
    11221 tcgcgtccga agtcgagcac gattcccggc gcgacgccgc tgttcacgag ttgacagccc
    11281 gaggtcttcc agacgccgcc gcaattcttc tgttgcggcg ccccggcgag aagcgcctcc
    11341 ggcgcgatca ccttcgcggg atcgccataa ttctcgcccg ccttggggaa cgtcgccacc
    11401 acccgtacgg gatgtatgag cgtccgcacg cggttgtcca cctgctcccc gaccgcagcc
    11461 gcactcaacc cgagcgccgc agcacagacc gtcattgtcc tgatcatcat cgcgcttttc
    11521 cttcggttcc catcctattt tccggtctcg tcattcaacg cgaaacgcac ggcggcgaat
    11581 tcatgggcgg gaagatccgc aagcgtgaca ccaccgggac cggttgcaaa ttttccgcag
    11641 acgctgccgg tgattcgggg cgtacccgcc gtcacgatct tcacctcggc agacttcgtc
    11701 cccatattct ccagcaacac ggcgtactcg ctgcgctttg cgttgtaccg cacaatctgg
    11761 tagatgcgcg gtgaagcggt ctcgacgtac acgtcgagcg ggcgtcccga cttcctccgg
    11821 acatcctcga gaatccgctt ccgcagatcg ccgcccttga acgcggcgaa atcaaccgcc
    11881 gccacgtcga tgccgaaatc ctccggacgg aagacgtacg aagcggttgc gtcatcgtgc
    11941 cgtatcggga tgccgcaccg cagcagaagg ttcgccgcca gcggctggga atagagctcg
    12001 gtgatgcgcc gttcttcaag cggctccgcc ggaagagtcg cctcgcggat acgccgcttc
    12061 gggaaatcga tacggagacc gtagggttcg gccccctcga acagttctgc cagtttccgg
    12121 tcgtccttca tattcgcgag atgcgcctcg atgtaccccg gctctgttcg atccttcccg
    12181 tagcagagca tgtacttgtg ccgcgccatg aggccgtccg cgatcgtcgc cttgtcgtag
    12241 agttcgcagc gccacgcagg aaccaccttg accttgcgtg gatacgggtc gttctcgtcg
    12301 aagatctcgt atcccttccc ttgcagccac tgtgcctgca tccgcgcgaa ctccgtcacg
    12361 ccctcgagcc cgatgccgcg gaatctcgcg tcgtcccagt acggcgcccc gctgatgcgg
    12421 acgaacggac gcgtctcccc tgcgaacgcc cgcagcagtt cgtccatgtc gacgccctcg
    12481 agatcccagt gggtcacgct gaggcagagc ccgatgcgcg tttggggatt cacctcgtca
    12541 accgccgcgc gcagttcccg cgcaagttcc accgcgacct cgcccgtcac gtcgacaaac
    12601 gccgtgcgca gcgcgttggg cttccctgtg aacgcccgga ccatatccgc atgcgtcacc
    12661 gggtggccga ccttcttcgc cacccgcgcg agatgacggt cgcaggcaca gccgacgaag
    12721 ccgcgggccg actgaatgaa gtcgtcgtcc atcaggatcg ccttcacccc cgtcgcggcg
    12781 atatcgcgca tgttctcgat gagcagccga cgcatcgcgg gatcgagcgg acagagcgca
    12841 ccgctcgtct tcccgtgcat tgagacgatg ttctgcgagt tcgcgaaccg tgtcttcccc
    12901 gccccgaccc gacgatcatt gccgtagccg aagccgttgc accagacgcg cacatcgagc
    12961 cccgcctcct cgaaataccg gacctcccgc ccgatcttcg caaaatacgg tttgcgactg
    13021 tccccgatgc catagtagtc gctatacgag acgagtaccg tggtgatccc ggcatcgcgg
    13081 atatccttca ggtacctcgc ttgattcgcc gcgttgaaat cttcgaccgg cacctgcgag
    13141 aagatctgca gcgtcgccag cagtagcgca tcagcgaaca tagagcacca agccgccgat
    13201 gacctggatc tgaagcgtat cgtccacggc ccgcaccttg acggtaccgc cagtcgaatc
    13261 aacccactcg gtgaagtcgc tcggcgttcc caacgtcgcc gcggtaaggg ttgccatccc
    13321 gccattcgga cgcttcccgc cataggcgat caccgccttc ttcggcagcg tcaccttgcc
    13381 gccgaaggtc gaattgagga acgtgtcgga actatcggta atcgtgaccg ttgcattctc
    13441 gccgagcgtg aaatcgccgg taaatcccga tgcgtttccg ccgagcacaa gattcgccgc
    13501 cgagatctcc ccgccgccgg tgagcgcgcc ggcgaccgca aacgtgccgt cgagtgtgag
    13561 cgcggacgtg tagccgacgt ccaccgaacc cgcgccggtc agggaaccgt actggtgccc
    13621 gtacccgcgc accgcaaccg acgtgccggc gtcgagttgc accttggccg gcgaaggcac
    13681 cgtccccgcc gggaccttgt cgagattccg ggaacgctgc gcatactccg ccgccacctc
    13741 ggcgtccgtc agcgcgacgt tgaagacctt gagttcgtca aaaccgccgg cgtaggtacg
    13801 ctgcacggag tgatccatat ttgtcaatcc gtcggcaccg acgatcaggc tgctgttgtt
    13861 ggcgacggtg aggttgtcgg gaaccgtcgt cgtcgccttg agctcaccgt caaaataaag
    13921 tttcagcacc ttgtcctcgt acgtgccggc gatctgatgc cacgctccgt cgaacagttc
    13981 ggtcgaggta taggctgcgg tccgggccgc tgccgcaccg ccattcgctg ctgaccaggt
    14041 gatcttggga ttgtcgccgt ccttgaagat ccagaaggaa tgtccgcggt tgtccatcca
    14101 ctggcatagg ttttctccat ggcgaagcag atacaccccg ttgtgaccga ggccgagatg
    14161 ttggtaatcg cgccatgtcg gagtgggctt gatccagaac gaataggaga ccgcctgatt
    14221 cccatacggg atgccgtgcg cggcattgaa acacgaaccg gaaaccgtga gatagtcgaa
    14281 cagccaccaa ccgttcacga aattcaaacc attgcccgtg atgccggcac aaccgctcag
    14341 cggcaccgga ttggcggatc cgtaggcaac gccggcgccg ggcgtcgccg tgaaggcgtt
    14401 tgtggaaaga tcgcaataca tgttgtccgg atcgtcgaag gcgaagtgcg ccacgagtcc
    14461 gcgctgggtc gaggggcgct tcatctcgat gtcgcccgcc tgcatccgga tcgtcacccc
    14521 gtcctgtccg ttcgtgcaga tcactccctt gagcttcagc gtcgtgtcgg cgttgtgggt
    14581 gatcgttccg tcatccgtga ccgccgtcac gtcgaagacc gcaccggcgg tgtagacctc
    14641 accgaccgta cggcggttgg acttcgtcga ccagttgtcg ccgtcctctg ccgcgccacc
    14701 ggccgatgcg cccttccaga cgatcacgtc ggtgtcgaac gcggtcgccc ccgcgttgta
    14761 ggcggcgaga atctcgtccg cgtcgagcgc gcggttatag accttgatgt cgtccacggc
    14821 cccggcgaag cggcgttgca cgtacgtgtt gtaatcaaca gcggcatggc cgaacatcag
    14881 cggcgaattg ttcgggacac cgacgttggc ggcgatcgtc gtctcggccc gcttcacgcc
    14941 gtcccagtag agcttcaaca cgcggttttc gtaggtggcg gttatcaagt gccagccacc
    15001 gtcgaaggcg gcccccagcg cataggtggc ggaggccgcc gccgcgttgt attcgccgac
    15061 cgaaaaagtg atcgtcggaa atcctgaacc gtccctgatg atgtagagac actcgtggtc
    15121 gtaggtcttg taccattcat gccccgccgt acagtgccgc agcagatagc agccggtacc
    15181 catgccgaga gtaccactcg tctgccagtt gggggacggc ttgatccaca gcgaataggt
    15241 gaccgcctga ttcccatacg ggatgccctg cgcggatccg aacgacgcgc ccggcacggt
    15301 catgtagttg ttctgccacc agcccgtcgc gatctgcatg ccgcggacga tcggcgcgtc
    15361 gcaggatgtc ggatctgcgg gcgtggcacc agagatgctg tcactcgtga tcgtgccgat
    15421 caccgcctgc tgcacattgt catacccgaa ctgcgtcggg ttgtcgaagg tgaagtgcgc
    15481 gacaagcccg ttctcggccg ccgccgcatc cataaacaaa cccaccgaca gcgctgtcca
    15541 cagcaacgtt gcttgacgtc tcatcggaac actccttttc attgtaagtc ggaataaaac
    15601 tttccattat cataccatcc catagggcag accggcaacc cccggctccc acggttagta
    15661 cgcattatgc ggggggggtc aaccctaaaa cttgtaatcg gttcgtgaca ggggcatgcg
    15721 aaggtcatgc gtttcagggt tccattgcgg cgataccgat accgatagtg atttggatgg
    15781 aaccatgaaa actgtgcccc cgcgcctctg tgcgaaaaaa cccctaactc cttgcctata
    15841 aattgacagg attacaggat tttcaggatt gaacagcatc atgttaatca tgtcctaaac
    15901 g
//
LOCUS       DAOSLE010000062        15773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3894_length_15773_cov_8.484222, whole genome shotgun sequence.
ACCESSION   DAOSLE010000062 DAOSLE010000000
VERSION     DAOSLE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_15773_cov_8.484222"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="PK576_05825"
     CDS             <1..1505
                     /locus_tag="PK576_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61148.1"
                     /translation="RRPEATTPIWICVPRDHAVSGSFWKAAGTEGDVDFGLTPQAQDE
                     AFTFIHFTDTHIGRGDLLKRFAEQTGTYPMRIAFAVNTGDLVGGVDFVPPEKAPAQYE
                     RYMEAARFPMPLFNLPGNHEHVGINYKGADREHPFYGKGLYPRLLGPMHYSWDWAGVH
                     CVALDGTSLPYQERLGSNQLAWLEADLALQPAEKPVLLFCHQSIPSLRDATLLAECLK
                     GRNVLGAFCGHLHRTFTATFAGFPVYHSGALSGAWWGGPNIDGTPQGFRLVQFRDGKV
                     KTVYTNWEGAYPVSIVEPLADTVQTGAVKVTVSVTDFGRPAEIAANYCGQPVALRLDS
                     REELWSFWSGTLDTRTAFDGDCTLTVTAICEQVSNTFAIRYLVENGRSGTYAAAEPAR
                     LCLQVRGGIDAPGEVQIHGEPLGSIPQGTTNETILAFAIPPERLRKLNRVTVLAARQG
                     QGWDRFNIGPVWIEYQGKRIYDLRYASFERHGVGGTHPADREKVLYYCLP"
     gene            complement(1521..7391)
                     /locus_tag="PK576_05830"
     CDS             complement(1521..7391)
                     /locus_tag="PK576_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE61149.1"
                     /translation="MKRNAQIVLILSACACLQVMVTSAQDIRAAGELFVHLDATNVTG
                     LSDGAYVATWPNHGTLKDFVPAFPGQGATYVSQLAGAAALQFDGTVTSTMVQNGHTNN
                     ASAGGVPLSILGTNVWSAELWFNNAAGGAIETLLTWTSRRSAGNRQLMEIRHYNTTDA
                     VEHYARNMTWSSQAPCFGQWQHLAVTRDALGMQRLYVNARLVSTVDMGGSNMLNLKPN
                     HALFAIGSVDAWGGWEKPLSAAVAVVRVHDGTLSPEDVLHNFMVERSRFGGGWNNPGT
                     GDWNEPANWENGAIPYQDAPLYINNGGTATYDGTPYENATQTAMLYGIEGGLTVADGI
                     FHALPLIMNDGLLRLGIAPGKTFTLGAVSGGSFTTLGKSLMLGDIAGATGSVSLASAG
                     RLTVARIQKGAGTALLTVDGGVLEATTSANNFLQGLTRAKVKANGICFDVPDQTAITV
                     PQNLLTDPDSPGGGLTKTGPGTLSLTGVNTLTGALQVTGGELVIQNNAFATNYASPIT
                     LANDGVIGWNQDGGAASIASNLTAGSVGTLKLYVPNAAENIDFSALPGMQLVTSGSFN
                     YTGVFTPYANRFVFRPDKGVMVFPQTIANLPDLPGCVEVRGTASGYVDLTADNSYTGG
                     TEIVSGGVIVSHANALGLGNDGVRDILCASGTVMRVQTLLSDPSFFSRVQAEPYAALQ
                     LSGAGLNQDIDLSDCPNLFTGSENITSRSYFNGVLTPYGNTYLLGNSGVDAATYYMGN
                     GFVITNLTDGPGGEPRAVLVSGIGAVDTRNQAAYSGGTRIENGARLIVTGDGGLGAVP
                     ATDDPDNIVIDGGILRTANPFISFAPTRGFTIGPKGAVFHAPGPLPAQLIIPGNLTGS
                     GTMRMTDMGWVTFTGTNNSYQGRVQLEGMVGAMMIGDGTNFSWTSTGGIVGTGTYGWL
                     YLNNSGENTFGDAFSGKGLLTKKGTGTITLTAAQSNRKLPVNTVIESGALRYGLANAL
                     SFGTADAGAVQINTGATLDLNGFSSALNGLAGTGCVTNRAGTAVTLQVGATDAASQFD
                     GQFAPGVTLEKIGTNTLLLTHAEPSRQNVSVAAGTLALYPGVALTNGITLTSDAAVRA
                     ASHRGLRAEYYDAVMPQGVPAIWPALGTTVEAVEELLSGRTPSLVTDCASFGDFFDSG
                     ELGTAFPGKYNQHSENYFVARYTGVFTAEEAGTYEFSLFADDGCMLFLDNTLVVNNRT
                     GKGTATGSITLEQRDYAILLFFYESAYSATLQLQMRGPSDAALSMLPQRLLKPASPAV
                     AVIGGILAAEGGRFEADSFGHLVLTDEGDYSAAGFGGPGITNAVIEKQGAGALTLTGT
                     SSFRGTALLSEGDIILANGTSHSNAFDVLNGTLTLSGGTSIDDAPVWIGSLNVAASGE
                     LALASKTFLRIYQDEAGIFAGTLSGGTADSVIAKDGPAELVLASDCSAYPGFWQVLAG
                     DLVITDNDALAADTVVTLERDGCLVFRCDGDVSFAGTVTGPGTIRNDGPGTLTLTGLT
                     TSGIEVPAGQTVILDGGEGDTSRELESDIVNNGTLRIAYPGGVLLGNPVSGTGTVSVA
                     SNTVVTVLRAGLLADDASLRLDGGTLTLHSGGTLGFNETTWQTNGVSRFVTNETGDVI
                     LEANPNVKSTVGSAFHKLRIPATNAWELNAVWRKGAGGGCGGGDGFAIMLQNDARGAS
                     ALGSSGSIGISTITPSFGYRFYLLSQKFAWVENGSAVGEFVTNLFSWGGGVFTADMAF
                     DGNQLTVTMSQGTKTLTVTNTNARAKLAAVGADAYVGLGSATGGCWGQQFIDSFSFSS
                     LWEPGYLFTNDLTLAANSASTIQAAARAVEGLPLEIGDLIFENGSALTAVPSADSDPD
                     LVFARFGNLTVRGASACHIDDAGWAAFRGTTWTFVPGAVLTVTGTVTLPETVLVAIEG
                     EIPQGRTDLANFRGADIMNAESVNFVLENDDGKTFLIFRDGILYVSRIQGSVLFLR"
     gene            8051..9106
                     /locus_tag="PK576_05835"
     CDS             8051..9106
                     /locus_tag="PK576_05835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE61150.1"
                     /translation="MSSGKQMLWWGRFDPAYSRNGVVRKHLTALGWTLRDFHPAVCAW
                     GDAEAALRRIPAPDCVWVPCFRQRDLAAARRWCDRRGVPLVFDPLISAYDKQVWERFK
                     YKDGSPAARKLLLWERGLFARADLVIADTVCHAAFFTEVLGVREEKIRVVCVGADETL
                     FRPAPERASGSPLDVLFYGSFIPLHGAQTIIEAARLDPGLPVRWSLLGDGPARPACQK
                     AAEGLTNVFFEDRIPYAQLPARIHRADILLGVFGTTRKAGRVIPNKVFQALASGRPVI
                     TRTSEAYPAAVRESEALSFVAPGDPQALADAVAAWAAQREHLHVRGQAAYKVYQSAFS
                     ADVVARQLRNVFASLSL"
     gene            complement(9098..10183)
                     /locus_tag="PK576_05840"
     CDS             complement(9098..10183)
                     /locus_tag="PK576_05840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOE61151.1"
                     /translation="MKIGIVTLCEANNYGGVLQAYALQTYLRRMGHESAVLPVDYAKR
                     RNSPLKVLERPVKTVMEAVKAKKFASFRAKHFRSPGVTACRVDAFLRQPPRFDAYVCG
                     SDQVWSRRACADSELRKYFFLDFGEVTARRISYAASWGAQTLEPAVRDDIRACLQKFT
                     ALSVREKSGQGIVADLGFTAQWLPDPTFLLEDEEWRRLAEDEPCTLGDNVLFQCEYRW
                     TPCVRLSEINRVLVRQFGWDTVIPFSDHPFRDAVCTRCLTPSQWVNGIRRASLVLTNS
                     FHGMVFSIIFRRPFIAVPLAGKYAGMNERFLSLAERLGLQDRLLTDPDPSRIIALAQA
                     PIDWESVYRHLNDWRREASTFLASALQ"
     gene            complement(10212..11396)
                     /locus_tag="PK576_05845"
     CDS             complement(10212..11396)
                     /locus_tag="PK576_05845"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOE61152.1"
                     /translation="MNLNPVHGDENTQPLLLCATEDCTGCSACSAICPESCVALLPDR
                     EGFLRPHVDSARCLRCGLCTNICPGLHPGLPAREPVAVYAAQNTCAAVREESSSGGVF
                     SLLAERVLERGGVVFGAGCDATFRVVHQEVEDAADLARLRGSKYVQSDMNDIFRRVRQ
                     HLGAGRQVLFSGSPCQVAGLRAFLGKAFDRLLCVDFVCHGVPSPRVFELWKRRLEKRS
                     RSAITGISFRNKACGWRQFSFAATFADGRTYRETVKEELYLRGFLSNLFLRPACHACR
                     FKEMKSGSDLTLADYWGVEKRFPCFRDNRGTSLVLANSAKGVEAFRSCASGVEFRDSD
                     YAHAVACNPSIVRSSRPHPDRARFFARLETHELGGLLLRMAKLSFRRRVKLFVKHALG
                     MI"
     gene            complement(11644..15477)
                     /locus_tag="PK576_05850"
     CDS             complement(11644..15477)
                     /locus_tag="PK576_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61153.1"
                     /translation="MIMCLAVAICGAASITFASTSEVGTYNWINTTAKTPGWGTDANW
                     ENGDWPLIKDTPAMTNSTFVFNGDLAIRQEIKDLEASYDVPAWLGTVLGSASHVLNCV
                     NSFSRRYTTIRDASSFEGSLGVAAGIGGTWLLLSDNAWTTTVANARMISNPAFLVRTA
                     GAKAVIERAWGRGRYLVNFANKTTHGTGAAEIEVIDSQGGPGADPHVVDGTFTVNGRS
                     YEAPALAGDPWMHLDASSSASLTLTLQDGTNYVTSWADVRANGLAAIQDGAAQCPRLE
                     TDAVSGLPIIDFGGFSPLTESVKAYGPAGTLIFNAGERSDIREVFVVFRDAPTKKPVP
                     SNTGIVCLQNFLCRAVGKGQYFTRRFSKRVNQNYYYGGLFDISVTTSGTKLTQPLASA
                     DVRLNGVRIEPEDVDDYSRHLNVLSVGLAHPVAADRFASTVDGHTGGIAFGEVLIYTN
                     ALTSAERRRVNAYLRAKWQPAEVAADIDFGRVTLNSGAQLKVNSGSVLVDEVNLNDTT
                     FVKQGAGGLRVKKLTGQTLPKVNVTGGRLDFADTCVTASFTAPDEPPVAPETWFDANC
                     GVSVFTTNVFDTEMEYVSQWVDRRDGTNLYGHTVVARRINTAGNYVNVGDSCFPTIRK
                     AYAAANGRDMMDFGPQRTNLTLNKTNFWVWAEPKDASAMYIRIEDAAPWYYNHEGYLV
                     ISPNTNRVITVGTVDFMPGDKGRLLGYDAYGHVQGAVWTVNGERVDPLSFVLEPGQVY
                     VIGYSLPVFGPAYGAGGSGVQNFAFLPPGYGSGTWGGIAIGEALTYPHKLTPLERRAT
                     EAYLLKKWLNREHPDAVHAQEGRALASIDYAEGATPELDVEGSLRVGVINGTGISFVK
                     SGSGSLDVGTLPHDVASIRVEGGSLSTGIDGMSDAFLHVDASDASSFEFRTEPGMEDQ
                     IVKWKDTRGNGMYAALPSEDRGLTNAVYRTATYPGLVEGLPCVDFLDSVEGAKPAGTT
                     LYVATVTNTSSGMCWYNAAGSQVTCPNLRECYIVFCDHEGESTGSFIGAKNSTFGARA
                     PNYTTIFPWYSQILTWIDQNTVTTNKYNFSENPGFHVLTIVTTNAADMVEGAYFCIDR
                     NGLRYGGCRIAEAVIFDKLLDDGRRQGIHNYLLKKWRAIGEGAVMTADLESVSVADGA
                     SLALESESRIRTAVLPGGDVTVSAPMEVTATAQSPWNYKVAFSAADSWNRLTVNGDLS
                     LPDYAAVTLTAPASLKLPTGLYPFITATQLTADISRWTLDNSDAPGLVTYKLVKTGNA
                     IAIHVSPKGVVFSIR"
BASE COUNT         2986 a         4742 c         4986 g         3059 t
ORIGIN      
        1 cgcggcggcc ggaggcgacg accccgatct ggatctgcgt gccgcgggat cacgcggtga
       61 gcggttcgtt ttggaaggcg gccggcacgg aaggagacgt ggatttcggt ctcactccgc
      121 aggctcagga cgaggcgttc acgttcatcc actttacgga tacccacatc ggacggggtg
      181 acctgttgaa gcgtttcgcg gaacagaccg gtacctatcc gatgcggatc gcgtttgcgg
      241 tcaacaccgg ggatctggtg gggggtgtgg attttgtgcc gccggaaaag gccccggcgc
      301 agtatgaacg gtacatggag gcggcccgct tcccaatgcc gcttttcaac ctgcccggca
      361 accacgagca tgtcgggatc aattataagg gcgctgaccg ggaacatccc ttttacggta
      421 aggggctcta tccgcgtctg ctcgggccga tgcactattc gtgggattgg gccggcgtgc
      481 actgtgtggc gctggacggc acctcgctgc cgtatcagga gcggttggga agcaatcaac
      541 tggcttggct ggaggcggat ttggcgcttc agcccgcgga aaaaccggta ttgctgttct
      601 gccaccagtc gatcccttcc ctgcgggatg caacgctgct ggcggaatgc ctcaaggggc
      661 gcaatgtgct cggtgcgttt tgcggccatc tccaccggac cttcaccgcg acgtttgcgg
      721 gttttccggt gtatcacagc ggagcgttga gcggcgcatg gtggggaggt ccgaacatcg
      781 acgggacgcc gcaggggttt cggctggtac agttccggga cggcaaggtg aagacggtct
      841 acacgaattg ggagggcgcg tatccggttt ccattgtgga gccgctggcc gacacggtgc
      901 agaccggagc ggttaaggtg acggtgtcgg tgacggattt cgggcggccg gccgagatcg
      961 ctgcgaatta ctgtggtcag ccggtagcgc tccggctcga ctcgcgcgag gaactctggt
     1021 ctttctggag tggcacgctg gacacgcgca cggcattcga cggcgactgc acgctgaccg
     1081 tcactgccat ctgcgagcag gtttcgaaca cgtttgcgat ccggtatctg gtagagaacg
     1141 ggcgttccgg gacctatgcg gccgcagaac cggcccggct ctgtctacag gtacggggcg
     1201 ggatcgatgc gccgggcgag gtccagattc acggcgaacc gctgggaagc attccacagg
     1261 gtacgacgaa cgagacgatc ctcgcgttcg cgatcccccc ggaacgcctg cggaagctca
     1321 accgggtgac cgtgctcgcg gcgcggcagg ggcaggggtg ggaccggttc aacatcggcc
     1381 cggtgtggat cgaatatcag ggcaagcgga tttacgatct gcgctatgcc tctttcgaac
     1441 ggcacggggt gggcggaacc caccccgcgg accgggaaaa ggtcttgtac tactgtttgc
     1501 cgtaaaaccg cagggcaggc tcaacgcagg aacaacacgg aaccctgaat ccggctgacg
     1561 tagaggatgc cgtcgcggaa aatcaggaag gtcttgccgt cgtcgttctc caggacaaag
     1621 ttgacggact ccgcattcat gatgtccgct ccgcggaagt ttgccagatc ggttctcccc
     1681 tgcgggatct cgccttcgat ggcgaccagc acggtctcgg gcaatgtgac ggtgccggtc
     1741 accgtcagca cggcgccggg aacaaaggtc caggttgtgc cgcggaaagc ggcccatccc
     1801 gcatcgtcga tgtggcaggc ggaggcgccc cgtaccgtga ggttgccaaa acgggcgaaa
     1861 accaaatccg ggtccgagtc cgcggatggg accgcagtca gggcggagcc gttttcgaag
     1921 atcagatcgc cgatttccag cggcaggcct tcgacggccc gagcggccgc ttggatcgtc
     1981 gaggcgctgt tcgccgccag ggtgaggtcg ttggtaaaca ggtagccagg ctcccagagc
     2041 gacgagaacg agaaggagtc gatgaactgc tgtccccagc aaccgccggt tgcactgccg
     2101 agcccgacgt aggcgtctgc gcccaccgca gccagtttcg cacgcgcgtt ggtgtttgtc
     2161 accgtgagtg tttttgtgcc ttggctcata gtcacggtga gctggttacc gtcgaacgcc
     2221 atgtcggcgg taaagacgcc gcccccccaa gagaacaggt tggtcacaaa ctcgcccacc
     2281 gcagaaccgt tctcaaccca ggcaaatttt tggctgagca aatagaatcg atagccgaag
     2341 ctgggcgtga tggtcgagat gccgatagag ccggaagaac ccagtgcgct tgctccgcgc
     2401 gcatcatttt gcagcatgat cgcgaacccg tcgcctccgc cacaacctcc tcctgcgcct
     2461 tttcgccaaa cggcgttgag ttcccaggca ttggtggcgg ggatgcggag tttgtggaac
     2521 gccgatccga cagtgctttt gacattgggg ttggcttcca gaatcacgtc cccggtctcg
     2581 ttggtcacga accgtgaaac gccgttggtc tgccatgtgg tctcgttgaa accgagcgtc
     2641 ccgccgctgt ggagcgtcaa cgtgccgccg tccagccgca gggaggcgtc gtcggcgagg
     2701 agcccggcgc ggagaacggt caccacggtg ttgctggcca ccgacacggt cccggttccc
     2761 gagaccgggt ttccgagcag gacgcctccg ggatacgcga tacgcagcgt accattattg
     2821 acgatgtcgc tttccagttc gcgggaggtg tcgccctccc ctccgtccaa gatcacggtc
     2881 tggcctgcgg gaacttcgat gccgctggtg gtcaggccgg tcagcgtgag cgtgccgggt
     2941 ccgtcattgc ggattgtgcc gggaccggtc acggttcccg cgaaggacac gtcgccgtcg
     3001 caacggaaga ccagacaccc gtcacgttcc agagtcacca cggtatcggc ggcgagcgcg
     3061 tcgttgtcag taatcaccag atcgcctgcc aagacctgcc agaaaccggg ataggcgctg
     3121 caatcactcg cgagcaccag ttcagccggg ccgtccttgg cgatcacact gtcggccgtt
     3181 ccgccgctca gggtccccgc gaagattccg gcctcgtcct gatagatgcg caggaaggtt
     3241 ttcgatgcca acgcgagttc accggaggcg gcgacattga gcgagccgat ccaaactggc
     3301 gcgtcgtcaa tcgacgtgcc gccgctgagg gtcagtgtgc cgttcagcac gtcaaacgca
     3361 ttggagtgcg acgtcccgtt cgccagaatg atgtcgcctt ctgaaagaag cgcggtgccg
     3421 cggaaagagc tggtgccggt gagcgtgagc gcacccgcgc cctgtttttc gatcaccgcg
     3481 ttggtgattc ccggtccgcc gaaacccgcg gccgaatagt ctccttcatc ggtcagcacc
     3541 agatgaccga aggagtcggc ctcgaaacgc cctccctctg cggcgagaat gcctccgatc
     3601 actgcgactg cggggctggc cggtttcagc aaccgttgcg gcaacatcga gagcgccgca
     3661 tccgagggcc cccgcatctg gagttggagc gtagcggagt aggcgctttc ataaaagaaa
     3721 agcaggatgg cgtaatcgcg ctgctccaag gtgatgctgc cggttgcggt tcccttgccg
     3781 gtgcggttgt tgaccaccag tgtgttgtcg aggaacagca tgcagccatc gtccgcgaag
     3841 agactgaact cataggtccc cgcctcttcg gctgtgaaca ctccggtgta gcgggcgacg
     3901 aagtaattct cactgtgctg gttatatttt ccggggaagg cggtgcctag ttcgccggag
     3961 tcgaaaaaat cgccgaagct ggcgcaatcc gttaccaggc tcggagtccg gccgctgaga
     4021 agctcctcga ccgcctcgac cgtggttccc agtgcgggcc aaatggccgg gacgccttgc
     4081 ggcatgacgg cgtcgtagta ttctgcgcgg agaccccggt gcgaggcggc ccggaccgcg
     4141 gcgtccgaag tcaacgtgat gccattggtg agcgcgacgc cggggtagag cgccagggtg
     4201 cccgccgcga ccgacacgtt ctggcgtgag ggttcggcgt gggtcagcag cagcgtgttg
     4261 gtgccgatct tttccagcgt gacgccgggc gcgaactggc cgtcgaactg tgaggccgca
     4321 tccgtagccc cgacctgtag ggttactgcc gtaccggcgc ggttggtcac acagcccgtg
     4381 ccagcgagac cgttgagtgc ggaggagaag ccgttgaggt cgagtgtcgc gccggtgttg
     4441 atctgcacgg cacctgcgtc ggcggttcca aaggagagcg cgttggccaa accgtaacgc
     4501 agtgcgccgg attcgatcac ggtgttgacc ggcagtttcc ggttgctctg cgcggcggtc
     4561 agcgtgatgg ttcccgtgcc ttttttcgtc aggaggcctt tacccgaaaa ggcgtcgccg
     4621 aaggtattct ctccgctgtt gttcagatag agccagccgt acgttcccgt gcccacgatg
     4681 ccgccggtgg aggtccacga gaagttcgtg ccatcaccga tcatcatcgc gcccaccatt
     4741 ccttcaagct gcacacgccc ttggtaactg ttgttggtgc cggtgaacgt gacccagccc
     4801 atgtccgtca tgcgcattgt gccggagccg gtgagattcc ccgggattat gagctgcgcg
     4861 ggcagcggac cgggggcgtg gaagaccgca ccttttgggc cgatcgtgaa cccgcgtgtc
     4921 ggggcgaacg agatgaacgg gttcgcggta cggagaatcc cgccatcgat cacgatgttg
     4981 tcgggatcgt cggttgcggg aacggcaccg agtccgccat caccggtgac gatcaggcgg
     5041 gcgccgttct caatgcgtgt gccgccggaa tatgcggcct ggttgcgggt atccacggcc
     5101 ccgattccgc tgacgaggac ggcgcggggc tcgccacccg gcccgtcggt caggttggtg
     5161 atgacgaatc cgttgcccat gtaatacgtg gctgcgtcta cgccgctgtt tccaagaaga
     5221 taggtgttcc cgtatggagt gagcacgccg ttgaaataac ttctggacgt gatgttctcc
     5281 gaaccggtga acaggttcgg acagtcagac aggtcgatat cctgattcag tccggcaccg
     5341 ctgagttgaa gcgcggcata gggttcagcc tgcacgcggc tgaagaagga cgggtcggac
     5401 agcagggttt ggacccgcat cacggtgccg ctggcgcaga ggatgtcgcg cacgccgtcg
     5461 tttccgaggc ccagcgcatt cgcgtgcgag acgatgaccc cgcccgaaac gatttccgtg
     5521 ccgccggtgt aactgttgtc ggccgtgagg tccacatatc cgctggcagt gccgcggact
     5581 tcgacacacc ccggcagatc cggcaggttg gcgatggtct gcgggaaaac catgacaccc
     5641 ttgtcgggtc tgaaaacgaa ccggttcgca tagggggtga agacgcctgt gtaattgaag
     5701 gagccggaag tcaccaactg catgccgggc agggcggaga aatcgatgtt ttccgccgcg
     5761 ttcggcacat agagtttcag ggtgccgacg gacccggccg tgaggttcga ggcgatcgag
     5821 gctgcgcctc cgtcctggtt ccagccgatg accccgtcgt tggcgagggt gatcggcgag
     5881 gcgtaattgg tggcgaacgc attattttgg ataacgagtt ccccgccggt cacctgaagg
     5941 gcgccggtaa gcgtgttgac gccggttagc gagagggtgc cggggccggt tttggtcaga
     6001 ccgccgcccg ggctgtcggg gtcggtcaac aggttttgag ggacggtgat cgcggtctgg
     6061 tcgggcacat cgaaacagat cccgttcgct ttcactttgg cacgcgtgag cccctgcaag
     6121 aaattattgg cagaggtggt ggcctccagc acaccgccgt ccacggtcag cagggcggtt
     6181 cccgcgcctt tctggatacg cgcaacggtc aggcgccctg ccgaagcgag cgagacggag
     6241 ccggtcgctc cagcgatgtc gccgagcatc agcgatttgc cgagggtggt gaacgatccg
     6301 cccgagacgg ccccgagggt gaaggttttg cccggggcga tgcccagacg cagcaagccg
     6361 tcattcatga tcagcggtag ggcgtggaag atcccgtccg cgacggtcag gccgccttcg
     6421 attccgtaca acattgcggt ctgggtggca ttctcatagg gagtgccgtc ataggtggcg
     6481 gtgcccccgt tgttgatata gagcggcgcg tcctgataag ggatggcccc gttttcccaa
     6541 ttcgcaggct cattccagtc gcccgtgccc ggattgttcc agccgccgcc gaagcggctg
     6601 cgttcaacca tgaagttgtg gaggacatcc tcgggactca atgtgccgtc gtgcacacgc
     6661 accacggcga cggcggcact cagcggcttt tcccatcctc cccatgcatc gaccgaaccg
     6721 atggcgaaaa gggcgtggtt gggcttgagg ttcagcatat tgctgccgcc catatccacc
     6781 gtgctgacca gacgggcatt gacgtacagc cgttgcatgc cgagcgcatc gcgcgtgacg
     6841 gcgagatgct gccactggcc gaaacagggt gcctggctgc tccatgtcat gttacgcgca
     6901 tagtgctcga ccgcatctgt cgtgttgtaa tggcggatct ccatcagttg acgattgccg
     6961 gcgctgcgac gcgaggtcca ggtcaggagc gtttcaatcg cacctcccgc ggcattgttg
     7021 aaccacagtt cagccgacca gacattcgtg cccagaatac tcagcggcac accgcctgcc
     7081 gaggcgttgt tggtgtgacc gttctgcacc atggtgctgg tgaccgtgcc atcgaactgc
     7141 aaggccgctg ccccggccaa ctgggacacg tacgtggcac cctggccggg gaaggcgggc
     7201 acgaaatcct tcagggtccc gtggttgggc caggtcgcga cataggcgcc gtcggaaagg
     7261 ccagtcacgt tggtcgcatc aagatggacg aagagttcgc ccgcggcacg gatatcctgt
     7321 gctgaggtta ccatgacctg caagcaggcg caggccgaca gaatcagaac gatttgcgcg
     7381 tttcgtttca taccactcct ttaccctttt tggaaatgta ggattattat gaactcaaca
     7441 attcgatccg tcaatcacgc actctgggag agagaggtaa tcggttcgtg acggggggga
     7501 agagtcatcg tgtacgtgta cgtgaacgag tacgtgtacg gggtatagcc aacgttcacg
     7561 ttcttcaaat cgctatcggg ttcggtatcg ctatcgggtt cgctatcggg ttcgaaatcg
     7621 actgttgcgc ccgcgcagtg gcccgcagtg acaagtgtaa tgatggcggc gatcccgatc
     7681 ccgatggcga tagcgatggc gccgcaatgg aaccctggaa cgcatgacct tcgatcgtac
     7741 ccctgtcacg aaccgattac gggagagaga ggacgggcgc atcctcttta ggccgaattg
     7801 cgttgagggg agcgggcttc gatgatatgc ttttgacatt cgctgtttcg tgcgcggtaa
     7861 ggcggaaaag gagattcatg cgtcacgcaa agcacgttct ttcaatcgcc ttcggcagac
     7921 ggtgtttcga ggcatatcag aagatccgaa aggagtgtgg cgaacgctga atgccgaacg
     7981 cggaacgcgg agggttgtcc ccggatgact tgcgcgttag gcgatcggac gggaagcgtt
     8041 gggcgttctc atgtcttccg gaaaacagat gctctggtgg ggccgttttg atccggccta
     8101 ttcgcgcaac ggtgtggtgc gcaagcacct gaccgcgctg ggctggaccc tgcgcgactt
     8161 tcatccggct gtctgcgcgt ggggcgatgc cgaagccgcg ctacgccgga ttcccgcgcc
     8221 ggattgcgtg tgggtgccct gtttcaggca gcgcgatctg gctgcggctc gccgttggtg
     8281 tgaccgccgc ggtgtgccgc tcgtgttcga ccccctcatc agtgcctacg acaaacaggt
     8341 gtgggaacgc ttcaagtaca aagacgggag ccctgcggcg cggaaactct tgctttggga
     8401 gcgcggactt ttcgcccgcg ccgatctggt gattgccgac accgtctgcc acgccgcttt
     8461 ctttacggag gttctgggtg tgcgggaaga aaagatccgc gtcgtctgcg tcggcgcgga
     8521 cgagacgctt ttccggcccg cgccggagcg tgcgtccggc tcaccgctcg acgtgctttt
     8581 ctacggaagc ttcattccgt tgcacggtgc gcaaacgatc atcgaagccg cgcggctcga
     8641 tccggggctt cccgtgcggt ggagcctgct cggcgatggc cccgcgcggc cggcctgcca
     8701 aaaggcggca gaggggctga cgaacgtttt ctttgaagac cgcatccctt atgctcaact
     8761 gccggctcgt attcaccgcg ccgatatcct gctcggtgtg ttcggcacga cgcgcaaagc
     8821 cggacgggtc atcccgaaca aagtgtttca ggcgctggcc tcgggacgtc cggtcatcac
     8881 gcgtacgtcg gaagcgtacc cggcagcggt ccgcgaatcg gaggcgctgt cctttgtcgc
     8941 gcctggcgac ccgcaggcac tggctgacgc ggtggccgcc tgggccgcac agcgtgagca
     9001 cctgcatgtc cgaggccaag cggcttacaa ggtgtaccag tcggcgtttt ccgctgatgt
     9061 cgttgcccgg caactgcgga acgtgttcgc ctcactctca ctgtaaggcg gaggcgagaa
     9121 aggtggaggc ttccctccgc caatcattca gatgccggta caccgactcc cagtcgatcg
     9181 gcgcttgggc aagtgcgata atccggcttg ggtccggatc ggtcaacagc cggtcttgca
     9241 gtcccagccg ctctgccagc gagagaaacc gttcattcat gcccgcgtat tttccggcga
     9301 gcggcactgc gataaatggg cgccggaaaa taatgctgaa caccatccca tgaaacgagt
     9361 tggtcaggac gagggaggcc cgccggatgc cgttgaccca ctgcgaaggc gtcaggcagc
     9421 gtgtgcacac cgcatcccgg aacgggtgat cggaaaacgg gatcaccgta tcccatccga
     9481 actgcctcac cagcacccgg ttaatctcgg aaagccgtac gcagggcgtc cagcggtatt
     9541 cacattggaa cagcacgttg tcccctagcg tacacggttc gtcctctgcg aggcggcgcc
     9601 actcctcgtc ctccagcagg aacgtgggat caggcagcca ctgggctgta aacccgaggt
     9661 ccgcaacaat cccctgtccg ctcttctcac gcacgctgag cgcggtgaac ttttgcaggc
     9721 acgcccgtat gtcatcgcgc acggcaggct ccaatgtctg cgcgccccag ctcgccgcgt
     9781 acgaaatgcg ccgcgccgta acctctccga aatcgagaaa aaagtacttt cgcagctcgc
     9841 tgtccgcgca ggcccgtcgg ctccagacct ggtcgctccc gcacacgtaa gcgtcgaagc
     9901 gcggaggctg ccggagaaac gcgtcaaccc ggcaagccgt tacgcccggc gacctgaaat
     9961 gcttcgcacg gaaagaggcg aactttttcg ctttgacggc ctccatcacg gttttgaccg
    10021 gacgctccaa cacttttaat ggagagttcc gccgcttcgc gtaatccacg ggcaagacgg
    10081 cgctctcgtg ccccattctc cgcaaatatg tttggagagc ataggcttgc agaacgccgc
    10141 cataattgtt ggcctcgcag agcgttacaa ttccgatctt cacatccccc ccctctaggt
    10201 tcagcttgcg gtcatatcat cccgagcgca tgcttcacaa aaagcttcac gcgccgccgg
    10261 aaagaaagtt tggccatgcg cagcagaagc cctcccagtt cgtgcgtctc cagccgtgcg
    10321 aaaaaacgcg cccggtccgg atgcggccgg gaagaccgca caatcgaagg gttacaagcg
    10381 accgcatgcg cgtaatcgga gtcgcgaaat tccacgccgg aggcgcatga ccgaaaagcc
    10441 tccacgcctt tcgcgctgtt cgccagtacg agcgaggtgc cccggttatc ccgaaaacag
    10501 ggaaagcgct tttcaactcc ccagtaatcc gccagcgtca ggtcgctgcc gcttttcatc
    10561 tccttgaacc ggcaggcgtg gcaggcgggg cgcagaaaga ggttggaaag gaagccgcgc
    10621 agatacagct cctcctttac ggtctcccga tacgtgcggc cgtccgcaaa agtggccgca
    10681 aaggagaact gacgccagcc gcaggccttg tttctgaaag aaatcccggt gattgcggaa
    10741 cggctgcgct tttccaaacg ccgcttccag agttcgaaca cccggggtga ggggacgccg
    10801 tggcagacga aatcgacgca cagcagtcgg tcaaacgcct tgcctaagaa agcgcgcagg
    10861 cccgccacct gacagggcga gccggagaag agcacctgcc gcccggcccc aagatgctgc
    10921 cgtacgcgcc gaaatatatc gttcatgtcg ctttgcacat acttcgatcc ccgcaggcgc
    10981 gcgaggtcgg ccgcatcctc aacctcctga tggacaacgc gaaacgttgc atcgcagcct
    11041 gcgccgaaaa cgacaccccc ccgctctagg acacgctccg ccaaaagcga gaagaccccg
    11101 cccgacgaac tctcctcacg cacggcagcg caggtgtttt gcgcagcata aacggcgacg
    11161 ggttcgcggg ccggcaggcc gggatggagc cccgggcaga tattcgtaca gagcccgcag
    11221 cgtaggcacc gcgcagaatc cacgtgcggc cgtaagaacc cttcgcggtc gggtagcagg
    11281 gcgacgcagg attccggaca gatggcgctg cacgccgaac aacccgtgca gtcctctgtc
    11341 gcacacagca gtaacggttg tgtgttttca tcgccgtgca ccggattcag attcataagt
    11401 aaatttacct tagcatatcc gtaacggtgt cttcaagcgg ccaaccttgt cgggatggcc
    11461 agcaaggact gggctgagat catcgcgaaa gttgaacagg acgcgaacgg actgggggcg
    11521 atagggcgca agcgccctct ccatctcgtt cccgtcggcg ttcagataaa cggtgcagag
    11581 tgcgagcgct aatcacgcac gtttgttttt caaaccagac ccctttgaag atcgtcaact
    11641 ccgtcagcga atactgaaca ccactccctt gggggacaca tgaatcgcaa tcgcgttgcc
    11701 tgtcttgaca agcttgtagg tcacgaggcc cggcgcatcg gagttgtcga gcgtccaccg
    11761 cgagatgtcc gcagtcagct gcgtcgccgt gatgaacggg tagagtccgg tcggcagttt
    11821 aagcgaagca ggcgcggtga gcgtgaccgc cgcatagtcg ggcagagaga gatccccgtt
    11881 caccgtgagc cggttccacg agtcggcagc cgagaacgcc accttgtagt tccacggtga
    11941 ctgcgccgtt gcggtcacct ccattggtgc ggacaccgtc acgtcgccgc cggggagcac
    12001 tgccgtgcgg atgcggcttt cggattccaa cgcgagtgat gcaccgtccg ccacggaaac
    12061 cgactcaaga tccgcagtca tgacggctcc ctcgccgatc gcccgccact ttttcagcag
    12121 atagttgtgg atgccctggc ggcgtccgtc atccagaagc ttatcaaaga tcaccgcctc
    12181 cgcgatccgg cagccaccgt agcgcagtcc gttgcggtca atacagaagt atgcgccctc
    12241 caccatgtcc gccgcgttcg tcgtcacaat cgtcagcaca tggaacccgg ggttttcaga
    12301 gaagttatac ttgttggtgg tcaccgtgtt ttggtcaatc caagtcagga tctgcgaata
    12361 ccaagggaag atggtggtat aattcggcgc acgggcgccg aaggtggagt tcttcgcgcc
    12421 gatgaaggat ccggtggact cgccctcatg atcgcagaac acaatgtagc attcgcgcag
    12481 attcgggcag gtcacctgtg atccggcggc gttgtaccag cacatccccg aactggtgtt
    12541 tgtgacggtc gccacataca gcgtagtgcc ggcaggtttc gcaccctcaa cggaatccag
    12601 aaaatccacg cacgggagcc cctcaacaag accgggatat gtcgcggtgc ggtagactgc
    12661 gtttgtgagg ccgcggtctt cactgggcag cgccgcgtac atcccgttcc cgcgcgtgtc
    12721 cttccacttg acgatctgat cctccattcc gggctctgtg cggaactcga agctggaggc
    12781 atcggaggca tcgacatgca gaaaggcgtc tgacataccg tcaattcctg tggagagcga
    12841 gccgccttcc acgcggatcg atgcaacgtc atgcgggagc gtgcccacgt cgaggctgcc
    12901 ggagccgctc ttcacaaacg aaatccctgt gccgttgatg acccccacgc gcaaggagcc
    12961 ttccacgtcc aactccggcg tggcgccctc ggcgtagtcg atcgacgcca gtgcccgccc
    13021 ctcttgtgcg tgcacggcgt ccggatgctc ccggttcagc cacttcttca gcagataggc
    13081 ttccgtggcg cggcgctcaa gaggcgtgag tttgtgcgga taggtgagcg cctcgccgat
    13141 ggcgatgccg ccccatgtgc cgctgccata gccgggcggc agaaacgcga aattctgcac
    13201 gccgctgccg cccgcaccgt aggccgggcc gaaaacgggg agactgtagc cgatcacata
    13261 aacctgaccg ggttccagca cgaacgagag ggggtccacg cgctcgccgt tgacggtcca
    13321 cacggcgcct tgaacatggc cgtacgcgtc gtagcccaaa agccggccct tatcgccggg
    13381 catgaaatcg acggttccga ccgtaatgac tcggtttgtg ttcggggaga tgaccagata
    13441 tccttcgtgg ttgtaatacc acggtgcagc atcttctatt ctgatataca ttgccgaggc
    13501 atcctttggc tccgcccaca cccagaagtt tgtcttgttc aacgtgagat tcgtacgttg
    13561 gggcccgaag tccatcatgt cgcgcccgtt cgctgccgcg taagccttac ggatcgtggg
    13621 aaaacagcta tcgccgacat tgacgtagtt accggcggtg ttgatgcgcc gggcgacaac
    13681 ggtgtggcca tagaggttgg tgccatcgcg gcggtccacc cattggctca catattccat
    13741 ttccgtgtcg aagacgttcg ttgtaaagac actcacgccg cagttggcgt caaaccacgt
    13801 ttccggcgca accggcggct cgtcaggcgc cgtgaatgag gcggttacac aggtgtccgc
    13861 gaaatcgagc ctgccgcctg tcacgttgac tttggggagc gtctgcccgg tcaacttctt
    13921 cacgcggagt ccgcctgccc cctgtttcac gaaggtggtg tcattaagat tcacctcgtc
    13981 caccagcacc gagccgctat tgacctttaa ttgcgcaccg gaattgagcg tcacgcggcc
    14041 gaagtcgata tccgcggcca cctcggcggg ttgccatttc gcgcgcaggt atgcgttcac
    14101 ccggcggcgt tccgccgacg tgagggcatt cgtgtagatc agcacctcgc cgaacgcaat
    14161 cccgcccgta tggccatcta cggtcgaggc gaaacgatcg gcggccacgg gatgcgccaa
    14221 cccaaccgag agcacgttca gatggcggga gtaatcatca acatcctcgg gctcgatccg
    14281 cacgccgttc aggcgcacat cagccgaggc gagaggctgc gtgagcttcg tgccggaagt
    14341 ggtgacgctg atgtcgaaga ggccgccgta atagtagttc tggtttacgc gcttggaaaa
    14401 gcggcgcgta aaatattgac ccttaccgac tgcgcggcag agaaaattct gcaggcagac
    14461 gatccccgta ttggagggga cgggcttttt tgtgggagca tcgcggaaca ccacgaacac
    14521 ctcgcggata tccgaacgct cgccggcgtt gaaaatcagt gtgccggccg ggccgtaggc
    14581 cttgaccgac tcggtgagcg gactgaaccc gccgaaatcg atgatcggca ggccgctcac
    14641 ggcgtccgtc tcaagacgcg ggcactgcgc ggcaccgtcc tgtatcgcgg cgagtccgtt
    14701 ggcgcggaca tcggcccacg acgtgacgta gttggtgccg tcctggaggg tcaacgtgag
    14761 cgaggccgag gacgaggcgt cgagatgcat ccacggatcg cccgccaagg cgggggcctc
    14821 gtaggaacgg ccgtttacgg taaacgtgcc gtcaaccaca tgcggatcgg cgccaggtcc
    14881 gccttgggaa tcgataacct cgatctcggc cgccccggtt ccgtgcgtgg tcttgttcgc
    14941 aaagttcacg agatagcggc ctcgccccca agcacgctcg atcacggctt tcgcgccagc
    15001 cgtgcgcacc agaaaggcgg ggttgctgat catgcgcgcg ttcgccacgg tcgttgtcca
    15061 agcgttgtcg gagagaagca gccaggttcc gccgataccg gccgcaaccc ccagcgaacc
    15121 ctcgaaagac gaggcgtccc ggatcgttgt gtaacggcgg gaaaacgagt taacgcagtt
    15181 cagcacatgg ctggcggaac ctaaaaccgt ccccagccag gcgggaacgt cgtagctggc
    15241 ctccaaatct ttgatctctt gccggatagc gaggtcgccg ttgaaaacaa atgtggaatt
    15301 ggtcatggcg ggcgtatctt tgatcagcgg ccaatcgccg ttttcccagt tggcatccgt
    15361 gccccaaccg ggtgttttgg ctgtcgtgtt aatccaattg taggtgccca cctcggacgt
    15421 gctggcgaac gtgatggaag cggcaccgca tatcgccacc gcgaggcaca tgatcatcgg
    15481 tctgccgagc gcgtaacggc acctcctgat gtttatccga aaccgacttg ccaaccccgt
    15541 gcattcgagc cgaactgttt tcatcgtttt atcctctttt tcagacaatg tcttttgtca
    15601 gttgcttgta ttgtactgta gccccagtgt atttggcaac cctcgcactt ttggttttgt
    15661 aatcagttcg tgacaggagg catgcgagtc gagcgcgcgt tttgtgcgta cgcaaagaga
    15721 gcgcgggcgt cttccgcgct acgcgcggat gtaggattta tttaggagtt agg
//
LOCUS       DAOSLE010000063        15577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3940_length_15577_cov_12.146888, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000063 DAOSLE010000000
VERSION     DAOSLE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3940_length_15577_cov_12.146888"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="PK576_05855"
     CDS             complement(<1..1959)
                     /locus_tag="PK576_05855"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOE61154.1"
                     /translation="MCNRRKKTEALYQVSFGTATCLAALLTVATSGLGSQGAGFPSRG
                     PDADIRRAFVTPPPGYGEVPFWWWTGERLDKERLLWQLEQLHKAGIPGTQINYAHTRS
                     DGWKTETVEPPIFSEAWWDVFVFMAKESAKRGMGIGMSGYTLDWPGRDNLFRQLGITG
                     DDLHARVLSAATRDVTGGRMPAGPGTDAVSATAFELKEGRIVPGTARSLDPQAAQWEL
                     PQGAWRIVTVTAALRPATLDPLNPESGQRVIKRFFDRFLARTPPEARSALNYFFQDEL
                     RLAGDKHLWSTEFAAQFKTRKGYDVRPKLAALFMDIGPETAKVRLDFNDVMVSLSEAY
                     YFKPIFEWHNSRGMIYACDPASRGRDPMEFGDYMRAMRWYTAPGFDTPGSSADLIKNK
                     VGSSIAHLYERPRVWVEGYHSLGWQASPTVLFDSSNRNFLYGTTLLNLHGLYYTTFGG
                     WWEWAPPCYHFRMPYWRHMPVFFKYFERLSYLLSQGDHVADVAIIQPHEPLVVDPARG
                     KAAQSCAYTLAADLIAQQIDFDFIDAESLARAEIVPPGESRTRLRPDTHARLCVAGEA
                     YRALVLPGMFAIRHASLEKALAFQQAGGRVIALGELPEATDRAGSDDPQTAALVKRLF
                     APDSGALHWTPPPAPQPAAAHAIRALIGT"
     gene            complement(2098..3378)
                     /gene="serS"
                     /locus_tag="PK576_05860"
     CDS             complement(2098..3378)
                     /gene="serS"
                     /locus_tag="PK576_05860"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE61155.1"
                     /translation="MLDIKRIREHFDEVAEGLRRRGAPVALAESARDLDAQRRTLVTQ
                     TDELKTLRNARSKEIGALKKAGQDTAVVQAEVRAIGDRITDLDAQIRTVEEALEKAML
                     MIPNVPCATIPTGPDASANRVAKVQGTAKTFDFKPLDHIALGEKLGLFDLPRGVKLTG
                     SGFPLLLGNGSKLQRALIQYMLDLHTERHGYTEMLPPFLVNTASMRGTGQLPKMAEDM
                     YHCEIDDFWLIPTAEVPVTNYYRDEIIDRPLPIYFTAYTPCFRREAGSAGKDTRGILR
                     VHQFDKVEMVKFVEPETSYAELETLVANAEDVLTGLGLHYRILELCSGDISFAAAKCY
                     DIELWAPGQQAWLEVSSCSNFEDFQARRLNCRYRDANGKTRLVHTLNGSGVALPRLMI
                     AILEQFQQADGSVVLPEAIRPYLRGLERLEPVSA"
     gene            complement(4508..5320)
                     /locus_tag="PK576_05865"
     CDS             complement(4508..5320)
                     /locus_tag="PK576_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOE61156.1"
                     /translation="MKRILILIFLSVSVLPGMASEEVWSGTKVISEPVKIVGARVRVL
                     PGAKIIFAGEGRIHMRDGSFTASRAEFLSESVLTNDFRMRFTECGFEARHCRFTGLRT
                     EKPDKRSFIYGFAFAISNGKMTLDHNTFVDCSAFMSAAVSHGMFTRNLFVRCAGGLAL
                     LNATQCRVEGNEFFDSPHEAMKLNTTRLSEIFRNRFTRCHIGMAFYAAKENRITGNAF
                     FASPIAFNLMHSGPDNVFSGNRYENVRQKYFNQGKSDGNRFFDDTPQPPTLK"
     gene            complement(5370..8144)
                     /locus_tag="PK576_05870"
     CDS             complement(5370..8144)
                     /locus_tag="PK576_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HOE61157.1"
                     /translation="MKCMIKQMMTVSVAMLSATVVAMPTLQPCVKEYRALESAAFNTA
                     ELPVFHQDQRQCVIGAAETFVKGERKIWDGKSAAANGVYLAIADSACGKKLIEAFSLD
                     VPAKPQGYAIKAEGRRVAIVGHDDVGALYGAVTFAQMSRNGRVEAAYVRDWPDVLYRG
                     SISFGRGLWKFRYGEKDEKGYSDAIKAGLDMMLRAKMNLFSDLFWLEEGMPDSTFPFF
                     RELGRYAAERGIWCNYYATTGVYIRRTAPKGMKFDDWKCVKLHCPWNDSYYCWADDAL
                     TEAAANRTVDFLVKAGMEKAVVNIHPVDGGSWQDPEEWSKRCEKCRAKWNDHERWKAS
                     INQFNIWIRVFRKRLPGAVVGSCIYPYQFNALMKPESERTAKWKESMPEYWDKLDKGL
                     ADKEFYFSSWITTPDVIKTVRELVPERPFHLSDTYPLTAGIFASYPRKIGSSCEGGRN
                     FATTQGTECYLQIETLLLVNEHLWDLHAPGTENYDGLTYYDGLKDHVAPDVIMKDSLG
                     RICRTFWGDVLAPYMQTVLSSGVMPEYLRDPAAKVKYWNKVRRDPDFDPTQPEKSKNS
                     GSLKYPPIIDTPELMLAQVEAAERCVHTLLEAEKRLDGLDRYKRKHFMRFLKYAPYWL
                     ATARTHHVVRLAGAALYDGDNAKALGILKEGRVRIEKDFDLAETTFKRLEEEADTLTD
                     PRRPRDASQTWKFDRKAATTVLDQTEASARITLEPRKIGAVVKLGVHRGGGDVCIKEY
                     FDQFENVKAEYFDSISLAELNRFDCVFLTQKQYEKDAFFTNVKTYVEKGGGGVFLEGE
                     LCGNVRFDVKTPFPEIVKTSPARVENFKRKMKFADGREGETMYVDYFRLTPGAKGEVL
                     AYGPNSGDVLAVRGVAGLGKVFFSGTLNIGSVADSYATRACPLFGANAEFAREAVEYF
                     TNVRLKEKDE"
     gene            complement(8209..8754)
                     /locus_tag="PK576_05875"
     CDS             complement(8209..8754)
                     /locus_tag="PK576_05875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE61158.1"
                     /translation="MNLTERTLGVKRVFEGRALTIDVAEIELPNGRRSTREIVRHRGA
                     AVILGQRPDGRFVLIRQYRRAVEGVLLEMVAGGLEEGESPETCARREMEEESGYPVET
                     LEKIGVIVPCPGYSEERLHLFYARLSQEPRPLRPDFDENLAPVVMTAEEIDAALDSGE
                     LIDGKSIALWLMWRRSTRRHS"
     gene            8921..11602
                     /locus_tag="PK576_05880"
     CDS             8921..11602
                     /locus_tag="PK576_05880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOE61159.1"
                     /translation="MDKHYDPKTVENKWYAWWEETGRFHAEAAQGGEPYSVVIPPPNV
                     TGILHMGHALNNTIQDVLVRWRRMQGRNTVWMPGTDHAGIATQNVVEKTLRKEGLSRR
                     DLGREAFLARVWEWREEYGGTIVRQLRKLGASCDWSRERFTMDEGLSEAVSEVFCRLY
                     DEGLIYRGNYIVNWCPRCHTALSDEESEHQDTQGHLWHIRYPIPDGGEVVVATTRPET
                     MLGDTAVAVNPADERYQALIGKRVTLPLMNREIPVVADDYVDRAFGTGVVKITPAHDP
                     NDFQVALRHNLPMLNVMNGDGTMNEEAGPYAGMDRFACRKQVIADLEAQGLLVKTDPH
                     QHAVGHCYRCDTVVEPRLSKQWFVRMKPLAEPAIEAVRTGKIVFVPQRWEKTYFDWME
                     NIRDWCISRQIWWGHRIPVYTCTPCGHEWAAKAAPDTCPKCGAAADQITQDEDVLDTW
                     FSSWLWPFSTFGWPADHPDLKFYYPTNDLATAPEILFFWVARMIMAGCKFMGDVPFRT
                     VYLHGTVRDDKGRKMSKSLGNSIDPLDIIETYSADSLRFSLMLITSTGMDVYVNMEKF
                     EIGRNFATKIWNAARFMRMHMEKLPDTDWRALANGELALDPALLRDDDRHMLATCENA
                     VRNVTGHLEAFRLQDGALAIYDVVWSQFCDWYLEYAKQDLNGADEARRRQVLAVMTDV
                     FAKALKLLHPYMPFITEELWHQMGYATESESIMRAPWPAPYTDAQRTAWGLSDAVTAY
                     VNARRELVTAGRALRADYNLAPSKFLHYVIQAMDEETAGRLKADAETLKQQLRSDNLE
                     VVAGGGEKAMPGTLCKLGTVYLPLEGVVDVKAEAARVKTELEKTRGFLKGVEAKLGNE
                     GFVAKAPAAVVEQQRAKQKELTETIARLEKLLASFGG"
     gene            11873..12973
                     /gene="ilvC"
                     /locus_tag="PK576_05885"
     CDS             11873..12973
                     /gene="ilvC"
                     /locus_tag="PK576_05885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOE61160.1"
                     /translation="MAKINFGGVVETVVTRKEYPLKKAQKVLKDEVIAVIGYGVQGPA
                     QSLNMRDNGIQVIVGQDKRFANDWKRAVKDGFKPGKTLFDIEEACARATIIMVLVNDA
                     AIRQVWPRIAPHLTAGKALYFSHGFGWVYRSLTRVKAGPDVDVIMVAPKGSGTSVRRN
                     FLAGVGINSSFAVAQDATGKALDRTLSIGIGVGSGYLFPTTFEHEVTSDLVGERGVLM
                     GALAGIMEAQYDVLRKNGHSPSEAFNETVEELTQSLIRLVDENGMDWMYSNCSATAQR
                     GALDWKPKFKKATLPVFKALYEAVKSQKEARHVIKVCGTADYKQKLDAELKVMRDSEM
                     WQAGAAVRALRPREKAKANAAKAVGVKGRGAN"
     gene            complement(13001..14164)
                     /locus_tag="PK576_05890"
     CDS             complement(13001..14164)
                     /locus_tag="PK576_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOE61161.1"
                     /translation="MNPVFYNAMVLREPYSGVEVTTHQLACALTRFGSLPLCICAPRG
                     HRPIPITSHTRMIRPYGTDRSRLLRILWEQTVLPVLLKRARAPLLHAPAYVAPRLAPC
                     PVVLTIHDLHVLTHPQFCRADNRLHYRLLLPPSIRRAAAVIVFSNYTRRVILDRFPEV
                     RDRLVLIPPGLTPNLARCTDHARLQSARQRYALPTSFLLFVGDLTQRKNILNLIRAFA
                     IVQGERPDLHLVLAGAADPATAAVIDNAIRQNAIRHRVSRIGYVSEEDLPVLYSLARA
                     FVFPSHDEGFGLPPLEAMACGCPVVCSGGAAVENCGDAAVNCDPRDRASIASAVSLLL
                     DHPAFRKEKIEAGYRKAAGFSWEKAARATDALYRSVLSAHGKHERPADEADGP"
     gene            complement(14184..14273)
                     /locus_tag="PK576_05895"
     tRNA            complement(14184..14273)
                     /locus_tag="PK576_05895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14237..14239),aa:Ser,seq:gga)
     gene            14517..15221
                     /locus_tag="PK576_05900"
     CDS             14517..15221
                     /locus_tag="PK576_05900"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE61162.1"
                     /translation="MASVRGVRGGRCGTSRGEWMLRYESKVWDAAPGEVLAGVDEAGR
                     GCLAGPVVAGAVAMSAETAERLYAGEFAELTDSKQLTVARREAFFGRLAEMPEVTWAT
                     GLCNAEEIDRLNILSATHLAMRRAVEALAVRPTHVLVDGLPVKGLPCASTAIVQGDAK
                     SFLIAAASVVAKVTRDRMMCELDARFPQYGFAAHKGYGVNAHMAALFKYGPCAEHRHT
                     FRPVQDADQKLPGFEY"
     gene            complement(15341..>15577)
                     /locus_tag="PK576_05905"
     CDS             complement(15341..>15577)
                     /locus_tag="PK576_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61163.1"
                     /translation="RYRDRERRPATRDIRPATKGWDEEFKKSVGRTAKPNVYGALLEF
                     YPFNCSFWGYPVSEHTDPAARPVNPQKTVVSDRV"
BASE COUNT         2935 a         4669 c         4945 g         3028 t
ORIGIN      
        1 ggtgccgatc agggcgcgga tggcgtgcgc cgcagcgggt tgcggtgcgg gcggcggggt
       61 ccagtgaagg gcgccggagt ccggggcgaa cagccgcttg acgagggcgg cggtttgcgg
      121 gtcgtcgctg cccgcgcggt cagtggcttc gggaagttcc ccgagcgcga tcacgcgtcc
      181 gccagcctgc tggaaggcca acgccttctc aagggatgcg tggcggatcg cgaacatgcc
      241 gggcagcacc agcgcacggt aggcttcacc ggccacgcag aggcgtgcgt gtgtatctgg
      301 acggaggcgt gtgcgtgact cgcccggcgg cacaatctcg gcacgcgcaa gcgattcggc
      361 atcgatgaag tcaaagtcga tctgctgtgc gatcagatcg gcggccaggg tataggcgca
      421 ggactgcgcc gctttaccgc gtgccggatc gaccaccaag ggctcgtgcg gctggatgat
      481 cgcgacatcc gcgacgtgat cgccttggct gagcaggtag ctgaggcgct cgaagtattt
      541 gaagaacacc ggcatgtgac gccagtaggg catacggaag tggtagcagg gcggtgccca
      601 ctcccaccag ccgccgaagg tggtgtagta aagtccgtgc aggttgagca gtgtggtgcc
      661 gtagaggaag ttgcggttgc tcgaatcgaa aagcacggtc ggcgatgcct gccagccgag
      721 actgtggtag ccctcgaccc acacgcgcgg ccgttcgtag aggtgggcga ttgatgagcc
      781 gaccttgttc ttgatcaggt cggcgctaga gccgggcgtg tcgaaacccg gcgcggtgta
      841 ccagcgcatg gcgcgcatgt agtcgccgaa ctccatcggg tcgcgaccgc gcgaggccgg
      901 gtcacatgcg tagatcatgc cgcggctgtt atgccactcg aagatcggtt tgaagtagta
      961 ggcttcgctg agcgagacca tcacgtcgtt gaagtcgagc cggactttgg cggtctcagg
     1021 gccgatgtcc ataaagagcg cagccagttt cggacgaacg tcatacccct tgcgtgtttt
     1081 gaactgagcg gcgaactccg tgctccagag atgcttgtcg ccggcaaggc gcaactcatc
     1141 ctgaaagaag tagttcagcg cgctgcgggc ctcgggcggt gtgcgcgcga ggaaccgatc
     1201 gaagaaacgc ttgatcacgc gctggccgga ctcggggttg agcgggtcga gcgtcgcggg
     1261 gcgcagcgcg gcggtaacgg tgacgatgcg ccatgcgccc tgcggtagtt cccactgcgc
     1321 ggcttgggga tcaagcgaac gcgcggtacc cggcacaata cggccttcct tcagttcgaa
     1381 ggcggtggcg gaaaccgcgt cggtgcccgg ccctgcgggc atacggccgc cggtcacatc
     1441 gcgcgtcgcc gcactcaaga cgcgggcgtg gaggtcatca ccggtgatac cgagttgacg
     1501 gaagaggttg tcgcggcccg gccagtcgag cgtataaccg ctcattccga tacccatgcc
     1561 gcgcttggct gattctttgg ccataaagac gaacacatcc caccacgctt cggagaagat
     1621 cgggggctcc accgtctcgg ttttccagcc gtccgagcgc gtgtgcgcgt agttgatctg
     1681 cgtgccgggg attccggcct tatgcagctg ctccaactgc cagagcaggc gctccttgtc
     1741 cagccgctcg ccggtccacc accagaaagg cacttcacca taacctggcg gcggcgtgac
     1801 gaacgcacgg cggatgtcgg catccggccc gcgacttggg aaccccgcgc cttgtgaacc
     1861 caagccgctg gtcgccacag tcagaagggc cgccagacat gtggccgtcc cgaaagaaac
     1921 ttggtaaagg gcctccgttt ttttccgtcg gttacacatg tctggtcctt tcgttctgct
     1981 agttcaggaa cagaaccgaa cctttactct tccatccacc acttctcgcc tttcgtcggc
     2041 tcatgcttac cgatgtcagg cgtttgtcct gttctgtccg aagcaaagca gggtcggcta
     2101 tgcggaaacc ggttcaagcc gctcgaggcc gcgcaggtaa gggcggatcg cctcgggcag
     2161 caccacggag ccgtcggcct gctggaactg ttccagaatc gcgatcatca ggcgcggcag
     2221 cgccacaccc gagccgttga gcgtatgcac gaggcgcgtc ttgccgttcg catcgcggta
     2281 acggcagttc aggcggcggg cctggaagtc ctcgaagttc gagcaggagg agacctccag
     2341 ccaggcctgc tggcccggtg cccacaactc gatgtcgtag cacttcgcgg ccgcgaagct
     2401 gatgtcgcct gagcagagtt caaggatgcg gtagtgcagg cccagtcctg tgagcacgtc
     2461 ctcggcgttc gcgaccaggg tctccaactc ggcgtacgag gtttccggct ccacgaactt
     2521 taccatctcc accttgtcga actggtgcac gcgcaggatc ccgcgcgtat ctttgcccgc
     2581 cgaacccgcc tcacggcgga agcaaggcgt gtaggcggta aagtagatcg ggagcggacg
     2641 gtcgatgatc tcgtcgcggt aatagttggt caccggcact tcggctgtgg ggatcagcca
     2701 gaagtcatcg atctcgcagt ggtacatgtc ctctgccatc ttgggcaatt gacccgtgcc
     2761 gcgcatggag gcggtgttga cgagaaaggg cggcagcatc tcggtgtagc cgtggcgttc
     2821 ggtgtgcagg tccagcatat actggatcag cgcgcgctgg agtttggagc cgttgcccaa
     2881 cagcagcggg aaccccgagc ccgtgagctt cacgccacgc ggcagatcga agaggccgag
     2941 cttttctccc agtgcgatat ggtcgagcgg cttgaaatcg aacgttttgg cggttccctg
     3001 tacctttgcc acgcggttgg ccgaggcgtc cggcccggtc gggatggtgg cgcacggcac
     3061 attcggaatc atcagcatgg ccttttccaa cgcctcctcg acggtgcgga tttgggcatc
     3121 aagatccgtg atccggtccc cgatcgcgcg cacctcggcc tgcaccaccg ccgtatcctg
     3181 tccggctttt ttgagtgcgc cgatctcttt cgagcgcgcg ttacgaagcg ttttgagttc
     3241 gtccgtctgc gtgaccagcg ttcgcctctg cgcatccaga tcgcgggcgc tttccgccaa
     3301 cgccactggt gcgccacgcc ggcgcaagcc ctcggcaacc tcatcgaaat gctctcggat
     3361 ccgtttgatg tctaacatag aaaacctcgc gttttttgaa aagaaaagta tattccatcc
     3421 tccctctccg cgcaagctta tgactgcgcc tgccgccgtg acctcacttg gcaaagtaaa
     3481 tgcgactggg gtggtcgcat tcacttcttc cgaacggggt tgatgcgacc agtggcattt
     3541 aattgctcca gggaggggcg gggggcggcg gagagggacg ggcggggtgc cttcccttcc
     3601 cccatggggg aaggtcgcat ttactttgag aatctcgttg ttgccttttc agggtaatcg
     3661 gttagtgaca ggggtgcgat caaaggtcat gcgtttcagg ggttccattg cggtgccatc
     3721 gctatcgcca tcgggatcgg gatcgccgcc atcattacac ttgtcgctgc gagccactgc
     3781 gtgggcgcaa cagtcgattt cgaacccgat agcgataccg acaccgatct ggagaacgtg
     3841 aacgtacacc gctgcgctgt gaacgtgaac gttggccata ccccgttcac gtactcgtac
     3901 gcgacaactc ttccccctgt cacgaaccga ttgccttttc cggcttgtct cagagtgtaa
     3961 atctggctac cctctatccg caatgtgtgt gtaagacgct tcaatcttac ctgtttgccg
     4021 cacgaatgcg aggtcgcctc caaaacctta agagttgcga tagccagtcg cacacgtgcc
     4081 cctgaaaacc cggtcgcatc gacctgttcc gaaaaaacat ggtgcgcccg ggaagggtgg
     4141 actgtgccca aaaacaaccc cggcgaaggc cgccgcgctg cgcggagcgc gttccgcgcc
     4201 gttttcggaa caactaaatg catattcgga gcaactgaat gcgaccgtgc gtcataaaga
     4261 cccagttttg gtcgcattta ctttgccaag tgacccctgc cgccgtgatc gaagacgcat
     4321 ccccggggcg ttgatcgttg gggggggagg tttcattcgg gaacgcgggt gtccccgtcc
     4381 gccgctatgc gccatcggcg cggggccgga ccgccgaaat cgaggacgag gacggggacg
     4441 ggggaaacga tcccgatgcc atcaggccgg gagggcaaac gtctgcttgc ccggacgagc
     4501 ccatttctta cttgagcgtg ggcggctgcg gcgtgtcgtc gaagaaacgg ttgccgtctg
     4561 atttcccctg attgaaatac ttttggcgga cgttctcata ccggttgccg gagaacacgt
     4621 tgtcaggccc cgaatgcatc agattaaagg cgatgggcga cgcgaagaac gcgttgcccg
     4681 tgatccggtt ttccttcgcc gcatagaatg ccatgccgat atggcaacgt gtgaagcggt
     4741 ttcggaaaat ctccgaaagg cgcgttgtgt tgagtttcat cgcctcgtgc ggggaatcga
     4801 aaaactcatt gccctccaca cggcactggg tggcgttcag aagcgcgagt ccgcccgcgc
     4861 accgcacgaa caggttccgc gtgaacatgc cgtgcgaaac cgccgcggac atgaaggccg
     4921 aacagtccac gaacgtgttg tgatcaagcg tcatcttccc gttcgaaatc gcgaatgcga
     4981 aaccgtagat gaagctgcgc ttgtccggtt tctcggttct gagccccgtg aacctgcaat
     5041 ggcgcgcctc gaaaccgcac tcggtgaaac gcattcggaa atcgttcgtg agcacgcttt
     5101 cagacaaaaa ttccgcccgc gaagccgtga acgaaccgtc ccgcatgtgg atccgtcctt
     5161 cgcccgcaaa gatgatcttc gcgcccggca acacgcggac gcgtgccccg acgatcttca
     5221 ccggctcgga tatgactttc gtcccggacc acacctcctc cgatgccata cccggcaaga
     5281 cagacacaga caggaaaatc aggatcagta ttcttttcat gaacgctcac acttgttgtc
     5341 accgttgtcg gcaatccgtt ttttggccgt cattcgtcct tttcctttag gcgcacgttc
     5401 gtgaaatact ccaccgcttc gcgggcgaac tcggcgttgg ctccgaagag cggacacgcg
     5461 cgcgtcgcgt agctgtcggc gacggaaccg atgttgagcg taccggaaaa gaataccttg
     5521 ccgagaccgg cgacgccgcg gacggcgagg acgtccccgc tgttcgggcc gtacgcgagc
     5581 acctcgcctt tcgcgccagg cgtcaggcgg aagtagtcca cgtacatcgt ttccccttcg
     5641 cgaccgtccg cgaacttcat tttccgcttg aaattctcca cacgcgccgg tgaggtcttc
     5701 acgatctccg ggaacggcgt cttaacgtcg aatcggacgt tgccgcaaag ttcgccctcg
     5761 agaaacacgc cgccaccgcc cttctccaca taagtcttca cgttcgtgaa gaacgcatcc
     5821 ttctcatact gcttttgcgt gaggaacacg cagtcgaacc gattgagttc cgcaagcgaa
     5881 atggagtcga aatactccgc ctttacgttt tcgaactggt cgaaatattc ctttatgcag
     5941 acatcgccgc caccccggtg gacaccaagt ttcacgacag cgccgatttt acgcggttcg
     6001 agtgttatgc gtgccgaggc ctcggtctgg tcgagcaccg tcgtcgcggc cttccggtcg
     6061 aacttccagg tttgggaggc atctctcggc cgccgcggat cagtgagcgt gtccgcttcc
     6121 tcctcgagcc gtttgaatgt cgtttcagcg aggtcgaagt ccttttcgat ccgaacacgg
     6181 ccttccttga ggattcccaa cgccttcgcg ttgtcgccgt cataaagcgc ggcgccggcg
     6241 agacggacca catgatgcgt gcgtgcggtc gcgagccaat acggcgcata tttcaaaaag
     6301 cgcatgaagt gcttgcgttt gtagcggtcg agtccgtcga gacgcttttc cgcctccaga
     6361 agcgtatgga cacatctttc ggccgcttcg acctgcgcga gcatcaactc gggggtgtcg
     6421 atgatcggcg gatacttgag cgagccggag ttcttcgact tctccggttg cgtggggtcg
     6481 aaatcgggat cgcgccggac cttgttccag tatttcacct tcgccgccgg atcgcgcagg
     6541 tactccggca tcacgccgga tgacaaaacc gtctgcatgt acggcgcgag cacgtcgccc
     6601 cagaacgtgc ggcagatgcg gccaaggctg tctttcatga tcacatcggg tgcgacatga
     6661 tccttcaggc catcgtaata ggtgaggccg tcgtagtttt cggtgcccgg cgcatggaga
     6721 tcccagaggt gctcgttcac gagcagcagc gtttcgattt gcaggtaaca ctcggttccc
     6781 tgcgtggtcg cgaaattccg ccccccctcg caactcgacc cgatcttgcg cgggtaggac
     6841 gcgaagatgc ctgcggtgag cggataggta tcgcttagat ggaacggacg ctctggcacg
     6901 agttcccgta ccgttttgat cacgtccggt gtggtgatcc aacttgagaa gtagaactcc
     6961 ttgtcggcaa gccccttgtc gagcttgtcc cagtattccg gcatggactc cttccacttc
     7021 gcggtacgtt cgctctccgg cttcatcagc gcgttgaact ggtacgggta gatgcacgag
     7081 ccgacgaccg cgccaggaag tcgcttgcgg aaaaccctta tccagatgtt gaactggttt
     7141 atgctcgcct tccagcgctc gtggtcgttc catttcgcac gacatttttc acagcgtttg
     7201 ctccactcct ccgggtcctg ccagctgccg ccgtctacgg gatggatgtt gacgaccgct
     7261 ttttccatgc cggccttgac gaggaaatcg accgtccggt tcgccgcggc ctcggtaagc
     7321 gcgtcgtccg cccagcagta ataggagtca ttccacgggc aatggagctt cacgcatttc
     7381 cagtcatcga acttcatccc cttcggcgcg gtacggcgga tgtacacccc ggttgttgcg
     7441 tagtaattgc accagatgcc gcgttcggcg gcgtaacgtc cgagttcgcg gaagaacggg
     7501 aaggtgctgt cgggcatgcc ttcctcaagc cagaagaggt cgctgaacag gttcatcttc
     7561 gcgcggagca tcatgtcgag gcccgccttg atcgcgtcgg aatatccctt ttcgtccttc
     7621 tcgccgtagc ggaacttcca gagtccgcgc ccaaaggaaa tcgagccgcg gtacagcaca
     7681 tccggccagt cccgcacgta tgccgcctcc acccggccgt tccgggacat ctgcgcgaac
     7741 gtcaccgcgc cgtaaagcgc gccgacatcg tcatgcccga caatcgcgac acgcctgcct
     7801 tccgccttga tcgcgtaccc ctgcggtttc gccgggacgt cgagcgaaaa cgcctcgatc
     7861 agttttttgc cgcaggcact gtccgcgatc gcgaggtaga cgccgtttgc cgcagcgctt
     7921 tttccgtccc agattttccg ttcgccttta acgaacgttt cagccgcgcc gatcacgcac
     7981 tgccgctgat cctgatgaaa aaccggcagt tccgccgtgt tgaacgccgc gctttcgagc
     8041 gccctgtatt ctttcacgca cggttgtagt gtcggcatgg ccacgaccgt cgccgacaac
     8101 atggcgaccg acaccgtcat catctgcttt atcatgcatt tcatatcggt ttctcagctt
     8161 ctacggacga acgcggtgcg gaatccaagc aattccgcat gagtagggct aactgtgccg
     8221 ccttgtgctt cgtcgccaca tcagccagag ggcgatgctc ttgccgtcga tcaactcgcc
     8281 agagtcgagg gccgcgtcga tctcctccgc cgtcatcacc acgggcgcga ggttctcgtc
     8341 gaaatccgga cgcagcggcc ggggctcctg cgacagacgc gcgtagaaga gatgcaggcg
     8401 ctcctctgaa tagccggggc acggcacgat cacgccgatc ttttccagcg tctccaccgg
     8461 atatccgctc tcctcctcca tttcacggcg cgcgcacgtt tccggagact ccccctcttc
     8521 cagccctccg gccaccatct ccaggagaac gccctccacc gcgcgccggt attggcggat
     8581 caacacgaac cggccgtccg gccgctggcc gagaatcacc gcagccccgc ggtgccgcac
     8641 gatctcgcgc gttgagcgcc gcccgttcgg cagttcaatc tccgccacgt cgatcgtcag
     8701 cgcccgtccc tcgaaaaccc gtttcacgcc cagtgtccgt tctgtcagat tcatatccgc
     8761 ttaagtgtcg cagaaacaac cgcaccggac aaccccgaaa aaagggtgcg gaaggactat
     8821 tctgcgccgc gattgtttgt cagcgcccgc ggtttctggt ttaatatccc ctttttacga
     8881 cccgtgacct gtaaccgtaa cgattaaccc gaactttttt atggacaagc attacgatcc
     8941 caagaccgtt gagaacaagt ggtatgcgtg gtgggaggag accggccggt ttcacgcgga
     9001 ggccgcgcag ggcggcgaac cctacagtgt cgtcatcccg ccgccgaatg tgactggcat
     9061 cctgcacatg ggccatgcgc tcaacaatac gatccaggac gtgctggtgc gctggcgccg
     9121 catgcaggga cgcaacacgg tctggatgcc gggtacggac cacgcgggca tcgccaccca
     9181 gaacgtggtg gagaagacat tgcgcaagga gggcctctcg cggcgcgacc tcgggcgcga
     9241 ggcgtttctg gcgcgcgtgt gggagtggcg cgaggagtac ggcggcacga tcgtgcgcca
     9301 gcttcgcaaa ctcggggcga gttgcgactg gtcgcgtgag cgcttcacca tggacgaagg
     9361 actgagcgag gccgtgtccg aggtgttctg ccgactctac gacgagggac tcatctaccg
     9421 cggcaactat atcgtcaact ggtgcccgcg ctgccacact gcgctgtccg atgaggagag
     9481 cgagcatcag gacacccaag gacatctctg gcacatccgt tacccgattc cggacggcgg
     9541 cgaagttgtg gtcgcgacca cgcgtcccga gaccatgctg ggcgacaccg ccgtggccgt
     9601 caatccggcg gacgagcgtt atcaggcgct gatcggcaag cgcgtgacgc tgccgctcat
     9661 gaaccgcgag ataccggttg tcgcggacga ctacgtggac cgcgctttcg gcacgggcgt
     9721 cgtgaagatc acgcccgcgc acgacccgaa tgactttcag gtggcgctgc gccacaatct
     9781 gccgatgctg aatgtcatga atggcgacgg cacgatgaac gaagaggccg ggccgtacgc
     9841 cggcatggat cgtttcgctt gccgcaagca ggtgatcgcc gacctcgaag cgcaggggtt
     9901 gctggtcaag accgatccac atcaacatgc ggtcggccac tgctaccgct gcgatacggt
     9961 ggtcgagccg cgcctctcca agcagtggtt cgtgcgcatg aagccgcttg ccgagccggc
    10021 tatcgaggcc gtgcgcaccg gcaagatcgt cttcgtgccg cagcgctggg agaagaccta
    10081 tttcgactgg atggagaaca tccgcgactg gtgcatctcg cgccaaatct ggtggggcca
    10141 ccgcatcccg gtctacacct gcacgccgtg cggacacgag tgggcggcca aggccgcgcc
    10201 ggatacctgt ccgaaatgcg gtgccgcggc ggatcaaatc acgcaggacg aggatgtgct
    10261 cgacacctgg ttctcctcgt ggctctggcc tttcagcacc tttggctggc ctgcagacca
    10321 tcctgacctg aagttctact atccgaccaa cgatctggcc accgcgccgg agatcctctt
    10381 cttctgggtc gcgcgcatga tcatggccgg ttgcaagttc atgggcgacg tgcccttccg
    10441 cacggtctat ctgcacggca cggtgcgcga tgacaagggg cgtaagatga gcaagagcct
    10501 gggcaactct atcgacccgc tcgacatcat cgagacctac agcgccgact cgctgcgctt
    10561 cagcctgatg ctgatcactt ccaccggcat ggacgtctac gtcaacatgg aaaaattcga
    10621 gatcgggcgc aacttcgcga ccaagatttg gaacgccgcg cgtttcatgc ggatgcacat
    10681 ggagaagctg cccgacacag actggcgcgc gctggccaac ggcgagctgg cgctcgatcc
    10741 ggcgctgttg cgcgacgacg accgccacat gctcgccacc tgcgagaatg ccgtgcgcaa
    10801 tgtgaccgga catctggaag cgttccgcct gcaagacggt gcgctcgcga tttacgatgt
    10861 cgtctggagc cagttctgcg actggtatct cgaatacgcc aagcaggacc tgaacggtgc
    10921 agacgaggcg cgtcgccgcc aagtgctggc cgtgatgact gatgtcttcg ccaaggcgct
    10981 caagctgctg catccctaca tgccgttcat caccgaagag ctatggcacc agatgggtta
    11041 cgccactgag tccgaaagca tcatgcgcgc gccgtggccc gcaccctaca ccgatgcgca
    11101 gcgcactgca tggggactga gcgacgcggt caccgcctac gtcaacgccc ggcgcgaact
    11161 ggtcaccgcc ggacgcgcat tgcgcgccga ctacaacctc gcgccctcca agttcctgca
    11221 ctacgtcatt caggcgatgg acgaagagac ggccggccgc ctgaaggccg acgccgaaac
    11281 gctcaagcaa cagttgcgct cggacaatct ggaagtcgtc gcgggcggcg gcgagaaggc
    11341 tatgcccggt acgctctgca agctcggcac cgtttatctg ccgctcgaag gggtggtgga
    11401 cgtcaaagcc gaagccgcgc gtgtgaagac cgagttggag aaaacgcgcg ggttcctcaa
    11461 aggcgtcgaa gccaagctcg gcaacgaggg gttcgtggcc aaggcgcctg cggccgtggt
    11521 cgagcagcaa cgcgccaagc agaaagagct gacagagacg atcgctcggc tggaaaaact
    11581 gctggcctcc tttgggggat gagaagatag acgaagacgc acgggcgtcc cgcctgtgaa
    11641 cacgggcaag atgcccgtgc tggatggtcg actgaaaaag gcgaccttgt ttttcctgat
    11701 ctgggggtcc aatcgccttt cggctgatcc cgtcgtcccg tcgtttaagc tgataaatgc
    11761 gcttgcgcgt gcgccgcaag gtggtatgat tacgtttctt ttttcgcccg accttctggc
    11821 catgtgcgcc gggttgttgg ggcggactga agttaaccga ggagtaaagg taatggccaa
    11881 gatcaatttt ggcggtgttg tggaaaccgt cgtgacccgt aaagagtatc ccttgaagaa
    11941 agcccagaag gtgctcaagg atgaagtcat cgccgtcatc ggctatggcg ttcaggggcc
    12001 ggcccagtca ctgaatatgc gcgacaacgg gattcaggtt attgtcgggc aggacaagcg
    12061 tttcgcgaac gattggaagc gggccgtcaa ggacgggttc aaaccgggca agaccctttt
    12121 tgatattgaa gaggcctgcg cccgggccac gatcatcatg gtcctcgtga atgatgcggc
    12181 gattcggcag gtttggccgc gcatcgcgcc gcatctgacc gctggcaagg cgctctattt
    12241 ctcccacggg ttcggctggg tctaccggtc gctcacccgc gtcaaagcag gtccggatgt
    12301 ggacgtgatc atggtcgcgc ccaaggggtc gggcacctcc gtgcgccgca atttcttggc
    12361 tggcgtgggc atcaactcca gttttgcggt ggcgcaggat gccaccggca aggcgttgga
    12421 ccgcacgttg tcgatcggta tcggcgtggg ttcgggttat ctgttcccca cgaccttcga
    12481 gcatgaggtg acctcggatc tcgtcggcga gcgcggtgtg ctgatgggtg ccctggccgg
    12541 catcatggag gcgcagtatg atgtgctccg caagaacggg cacagtcctt cggaggcgtt
    12601 caatgagacg gtcgaggagc tgacccaaag cctgatccgt ctggtcgatg agaacgggat
    12661 ggattggatg tattcgaact gttcggccac tgcccagcgc ggcgcgctgg actggaagcc
    12721 gaagttcaag aaggccaccc tgccggtgtt caaggcgttg tacgaggcgg tgaagagcca
    12781 gaaggaagcg cgtcacgtga tcaaggtgtg cggcaccgcg gattacaagc agaagctcga
    12841 cgcggagctg aaggtgatgc gggattccga gatgtggcag gctggcgcgg cggtgcgcgc
    12901 gttgcggcca cgtgagaagg ccaaggccaa cgcggcgaag gctgtcggcg tcaaagggcg
    12961 cggagccaac taaggttcgc gcccgcggcg cggaacggat tcaaggcccg tcggcttcgt
    13021 cggcgggcct ttcgtgtttc ccgtgcgcgc tcagcacgga tcggtacagc gcgtcggtgg
    13081 cgcgtgcggc cttttcccag gaaaacccgg cggctttccg gtagccggcc tcgatcttct
    13141 ctttacggaa cgccggatga tccaacaaca ggctgacggc actggcgatc gacgcgcggt
    13201 cacgcgggtc gcaattgacc gccgcatccc cgcagttctc gacggctgcg cctccggagc
    13261 agacgaccgg gcacccgcag gccatggctt cgagcggcgg caacccgaac ccttcgtcat
    13321 gggaggggaa aacgaaggcc cgcgcgagcg aatagagcac gggcagatcc tcttcgctca
    13381 catacccgat tcggctgacg cgatggcgga tggcgttttg ccggatcgcg ttgtcaatca
    13441 cggctgcggt ggcggggtcg gccgcgcccg ccaaaaccag atgcaggtcg ggccgttcgc
    13501 cctgaacgat cgcaaaagcc cggatcaggt tgaggatgtt tttgcgctgc gtgagatcgc
    13561 ccacaaacag caggaacgat gtgggcagcg cgtaacgctg gcgcgcggac tggagccgcg
    13621 cgtggtcggt acaccgggcc aggtttggcg tgagtccggg cggaatgagg acaagccggt
    13681 cccggacttc cggaaaccgg tcgagaatga cgcgccgcgt gtagttcgaa aaaacgatga
    13741 ctgcggccgc ccggcggatc gagggcggaa gcaggaggcg atagtggagc cggttgtcgg
    13801 cacggcagaa ctgcgggtgt gtcaggacat gcagatcatg aatggtgagc accactggac
    13861 aaggcgcgag ccgcggcgcg acatacgccg gcgcatgcag cagcggcgcg cgcgcgcgct
    13921 tcagcaacac gggcaggacc gtctgctccc agaggatgcg caacaggcgt gatcggtccg
    13981 tgccgtatgg ccgaatcatg cgcgtgtgcg aggtgatcgg gatcggacgg tgcccccggg
    14041 gtgcgcaaat acagagcggc agcgaaccaa agcgggtcag cgcgcaagcc agctgatggg
    14101 tggtaacttc gacgccggaa tacggttcgc gcagcaccat ggcgttatag aaaacgggat
    14161 tcatggattc accgcaccgg gattggcgga gagggaggga ttcgaaccct cggtacccgt
    14221 aagagcacac agcatttcca gtgctgcacc ttcggccgct cggtcacctc tccccgaaaa
    14281 gttcccacgt tataccgggt tactgtgtgg aaaacaatcc taaaatcctg gacgcttttt
    14341 tcggcagatt ggcgcagatt tacgttttgc cggttgccgc gccttgatat tttacattac
    14401 cgatggcgat gcggtgtgtt accattcgtt tcgtaagtgg aaacggttca ggtgcagagg
    14461 ggtgcaccgg gaaaggggat acgggcacgg atcacaggcg gaaccggttt tccgggatgg
    14521 catctgtgcg cggcgtgaga ggcggccgct gcggcacgag caggggggaa tggatgttgc
    14581 ggtacgagtc aaaggtatgg gatgcggcac ccggcgaggt gctggccggg gtggacgagg
    14641 ccgggcgcgg ctgccttgcc gggccggtgg tggcaggcgc ggtcgcgatg tcggcggaga
    14701 cggccgagcg gttgtacgcg ggagagttcg cggaactgac ggactcgaaa cagttgacgg
    14761 tggcgcggcg cgaggcgttt ttcgggcggt tagcggaaat gccggaggtg acgtgggcca
    14821 cggggctatg caatgcggag gagatcgatc ggctcaacat cctttcggcg acgcatctgg
    14881 cgatgcggcg ggcggtggag gcgttggcgg tacggccgac acatgtgctg gtggacgggc
    14941 ttcctgtgaa ggggctgccg tgcgcctcga cggcgatcgt gcagggcgac gcgaagagtt
    15001 ttctgatcgc ggcggcgagc gtggtggcaa aggtgacgcg tgaccggatg atgtgcgaac
    15061 tggacgcgcg gttcccgcag tacggcttcg cggcgcacaa ggggtatggc gtgaacgcgc
    15121 acatggcggc gctgttcaag tacggtccgt gcgcggagca ccgccacacg ttcagaccgg
    15181 ttcaggacgc agaccagaaa ctgccggggt ttgaatactg aacgggccgc gtttacgcac
    15241 tgtcggcggt ccgagacgtc ccgccgtact ttacctgcgg agttagaaaa ctggagctgg
    15301 cgccggcttc ggggcgacac ggcaaagtac ggtcggcgat tcagacgcgg tcggacacca
    15361 cggttttttg tggatttacc ggacgggcgg cggggtcggt gtgttcactc acgggataac
    15421 cccaaaagga gcagttgaac gggtagaatt ccagcaaagc cccgtaaaca ttgggttttg
    15481 cggtgcgtcc gacgctcttt ttgaactctt cgtcccagcc cttcgtcgcg ggtcgaatgt
    15541 cgcgggtcgc gggtcgacgt tcccgatccc gatagcg
//
LOCUS       DAOSLE010000064        15519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_3965_length_15519_cov_8.244245, whole genome shotgun sequence.
ACCESSION   DAOSLE010000064 DAOSLE010000000
VERSION     DAOSLE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15519
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_15519_cov_8.244245"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..351
                     /gene="secE"
                     /locus_tag="PK576_05910"
     CDS             148..351
                     /gene="secE"
                     /locus_tag="PK576_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOE61164.1"
                     /translation="MSKVKESIRSFVAYLGEVGVEFKKITWPDRQELVDSTVVVLVFI
                     VILAVVVLCCDKTILFFLQLIHA"
     gene            365..928
                     /gene="nusG"
                     /locus_tag="PK576_05915"
     CDS             365..928
                     /gene="nusG"
                     /locus_tag="PK576_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOE61165.1"
                     /translation="MTKQWFVLHTLTGQEQKVKRSIEARVRIEEMGEYIGEVKIPTEK
                     VSEVKNGVKKTVTRKFFPGYVLINLALYDEARNVIEPTWRFLKETGGVIGFIGGSRPL
                     PLSPDEVDSIVNQAAEKKEKVVPKVQFEPGEEVKITDGPFMSFSGTVDEVDPDRGKLK
                     VSVAIFGRTAPVELEYWQVERAVSDRA"
     gene            1040..1465
                     /gene="rplK"
                     /locus_tag="PK576_05920"
     CDS             1040..1465
                     /gene="rplK"
                     /locus_tag="PK576_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOE61166.1"
                     /translation="MAKKITGTVKLQIPAGAANPAPPVGPALGSQGINIMAFCKEFNA
                     KTQDKAGLIIPVVITVYADRSFSLVFKSPPASVLLKKEAGLAKGSGVPNRDKVGKVTR
                     EQVRKIAALKKADLNASSEEAAMRMIEGTARNMGIEVVD"
     gene            1484..2176
                     /gene="rplA"
                     /locus_tag="PK576_05925"
     CDS             1484..2176
                     /gene="rplA"
                     /locus_tag="PK576_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOE61167.1"
                     /translation="MAKHGKRYVDALKKAPQGPVSLEEAVAFVKANPGAKFDETVEVA
                     MRLGVDIRKSDQTVRGTVTLPHGSGKKVRVVVFAAGNHADEAKAAGADEVGYEDLIEK
                     VKNGWTDFDVAIATTDAMKEVRKIARVLGPRGLMPNPKTGTVTDDTAAAVKAAKGGKV
                     DFRMDRTGNVCVLCGKRSFDAEKLVENIKAVVDAVRAERPAGAKGTFIRSLTVSATMG
                     VGVRVLVKEAAE"
     gene            2189..2713
                     /gene="rplJ"
                     /locus_tag="PK576_05930"
     CDS             2189..2713
                     /gene="rplJ"
                     /locus_tag="PK576_05930"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOE61168.1"
                     /translation="MRTEKVSILNEVIERVKDSDFCFVLNYGGLSVAQLTALRKELAK
                     HDARAMVVKNTYLGRVAKEQGWADVSGMLTGPTAIITGRGDVAEVAKALVEFVKKNDK
                     AAIKGANLDAAMLSAADVEALSKLPSKDAMRGILLGTLQAPATSLVRVLNAPLLNVLY
                     ALKAYEEKKNGSAA"
     gene            2866..3234
                     /gene="rplL"
                     /locus_tag="PK576_05935"
     CDS             2866..3234
                     /gene="rplL"
                     /locus_tag="PK576_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOE61169.1"
                     /translation="MTNEEVIDFLSNLSVLEISKLVKALEEKWGVTAAAPVAVAAAPA
                     AAAAPVEEKTEFDVVLVEAGANKISVIKEVRAITGLGLKDAKDLVEAAPKPVKEGVAK
                     EEAAKLKEQLEKAGAKVEVK"
     gene            3577..7428
                     /gene="rpoB"
                     /locus_tag="PK576_05940"
     CDS             3577..7428
                     /gene="rpoB"
                     /locus_tag="PK576_05940"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOE61170.1"
                     /translation="MVDRKVFGKLKPVMQPPDLIAIQTGPYQDFLQRDVAPLKRENKG
                     LQAIFHEVFPIDSFDGRYTLDFVKYDLGEPKMDALTALVEGNTYAAPLHATFRLKDGD
                     EIREEDVYMGEIPLMTRDGAFVINGAERVIVSQLHRSPGICSERTQHPNGQTLYSVRI
                     IPDRGSWIEIQFDSSDLMWVYMDRRRRRRKFFVTTFLRTLGYGTDEDILKLFYTFTSL
                     PVNKEFTDEELEVLVMKDDLIDVESQAVLARRYDPITPALLEQIGKAGFSKIEVVDVS
                     FDGGVLIKTLREDAKANIYSEEDALKDIYKRLRPGDPATASNAKALLKRLFFEPRRYD
                     LGKVGRHKINQRLGLSGKVPDTLRTLDESGVDVVEAVRLLLKIYTGEETVDDIDHLGS
                     RRVRTAGELLENQCRVGLARTERLIRERMTLFDPASGVLAPARLVNSKSLSAVVQDFF
                     GRSQLSQFMDQTNPLSGLAHKRRLSALGPGGLSRERAGFDVRDVHASHYGRICPIETP
                     EGPNIGLISSLCIYSKINEYGFIESPYRVVRNGKVTKEVQYLSADKEEEFVIAQANSE
                     LKSDGSFVSQRVTCRYKTEFVVVDRSSVQYMDVSPMQVISIAAGLIPFLEHDDANRAL
                     MGANMMRQAVPLLQSESPFVATGLEARAASDSCVTVLATEDGVVAYASATEIIVTPDG
                     KMPPKTKKGEAIKEAGRYSLQKFRRCNAGTCFNQKPVVEVGQKIRKGDVLADGPATYG
                     GELALGKNLLVAFMPWTGYNFEDAILVSERIVREDIFTSIHIQEFEVGARDTKLGPEE
                     ITRDIPNVGEEALKNLGPDGVVRIGAEVRPGDILVGKITPKSETELAPEERLLRAIFG
                     EKAADVRDTSLTVPSGVYGIVMDVKVTSQQDAHRPFLSLTASSEEKREVRDIEEERKK
                     KQAALEEELTTALSNILLGEKIPLDVLDVENGEIIIPANRKITKTLLARLAKAHDHIQ
                     IENSPVQQKIEEIINGFRPKFADLAEQSSNDFGAELDGIIDEGGVIKQVRVYIAEKKN
                     LSVGDKLAGRHGNKGVVAKILPDCDMPFLPDGTPVDIVLNPLGVPSRMNLGQLFETYL
                     GVACKVLGFHASSPVFDGVSEEEIDALLRQARKVQESEIGGSAWICEDGKTVLYDGRT
                     GEPFDQRVVVGHIYMLKLHHLVTDKIHARATGPYSLVTQQPLGGKAQYGGQRMGEMEV
                     WALEAYGVAYALQELLTVKSDDVSGRTRIYEAIVKGQNTLDAGMPESFSVLMHELRGL
                     CLDMKLLGSDTAGPTPELSRI"
     gene            7450..11640
                     /gene="rpoC"
                     /locus_tag="PK576_05945"
     CDS             7450..11640
                     /gene="rpoC"
                     /locus_tag="PK576_05945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOE61171.1"
                     /translation="MNPYASREMIGGTYDANAHYDAVSITLASPETIRSWSHGEVRNP
                     ETINYRTYRPERDGLFCERIFGPTRDWECNCGKYKRIKHKGVVCDRCGVEVTVSRVRR
                     QRMGHIELAVPVSHIWFFKCSPSRIGLILDKTANELERVLYYEDYMVIDPGEKSGLEK
                     AQLITESEYHDYVEKYGESFTAMMGAEAVRRVLNDVDLDLLMQTLEEQIENTRSKQTR
                     KKITKRMKIVEGFRTSGARPEWMILDCLPVIPPELRPLVPLEGGRFATSDLNDLYRRV
                     INRNNRLKNLLSLKTPDVIIRNEKRMLQEAVDAVFDNGRHGRAVAGAGNRPLKSLSDM
                     LKGKTGRFRQNLLGKRVDYSGRSVIVVGPELKITQCGLPKKMALTLFEPFIIRRLKEK
                     GICHTVRTARKMIERHDEQVWDILEEVTKGKTVMLNRAPTLHRLSIQSFEPVLIEGEA
                     IRIHPMVCTAFNADFDGDQMAVHVPLSVEAQLESRLLMLTSNSIFSPASGKSIMTPTQ
                     DIALGIYYLTIPSQSDKLANKIAGKVPAGAERLPLFNTTAEVDSALAEGAITYHSRIR
                     LRNPDFGTQDRRFGNPEPSVIETTAGRVIFNEVWPDELGFVNDKVDKNKLGDLILHCH
                     QVAGHARTVKALDELKTLGFKHACLAGVSMGLKDMIRPKNKDEIIAQAHSEVEKVEAQ
                     FQTGVITDGERHNKIIDIWTAATEKISDELYKAIDRNVTESVSEPNETNPVFMMVDSK
                     ARGSKLQIRQLAGMRGLMANPSGEIIERPITASFREGLSVLEYFISTHGARKGLADTA
                     LKTADAGYLTRKLVDVSQDVIITIQDCNTVNGIEVEAVEEGDEMLIPLRTRVLGRTAL
                     YDIRHPATMELLVAANAEIDEAACKALEDAGIERVWIRSGLTCDAPHGMCAKCYGRDL
                     SSGKLVEIGTAVGIIAAQSIGEPGTQLTMRTFHIGGTASTVSKIPEIVLKNAGFIKYV
                     DLRIVQNDEGNTVVLNKNGTIEIVDANGNEIDTYNLQMGTVLLAADGAAVKAGQRVAV
                     WDPHSVPILADKHGVVSFQDFVDDVSVRSDQDAETGATTLLVLDTKEDLHPQIIITDA
                     KTGEILGFYHIPVGAVVMVKEGTKVSPGMLLAKTPRKEAKTRDITGGLPRVAELFEAR
                     QPKDAAEISRIEGVVDFGEHQRGKRCLIVRDEGGHDQEHLIPMGKQVVVFKGDRVKKG
                     QQLTEGPVVPQEILDVCGPQELQRYLVNEVQQVYRLQGVEINDKHIEIIVRQMLRKVR
                     ITNPGDTDFLWGEQVSRQKFLEVNEQVLASGKRAAEAQPALLGITKASLETDSFISAA
                     SFQDTTRVLTDAATMGRVDELRGFKENVILGHLIPGGTGFPLHRHIKLVPLADPISEE
                     EMAALQADAQAQFDEVFN"
     gene            complement(11707..14655)
                     /locus_tag="PK576_05950"
     CDS             complement(11707..14655)
                     /locus_tag="PK576_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOE61172.1"
                     /translation="MIRYLRAVIALSAVVFTAALVRAEGLALESPGQKLQAVFALSPE
                     GTLTYRVTFGGVPVILPSKLGFTTVDRTDLTLGYALVSAKRATIHQTWKNPFGERAQV
                     TDAYNALTLILRNRAANDLTLHLECRAYDAGLAFRYVFPQQSLTQLLFAKENTEFIFT
                     GDHRAWPVYSAQAAYKPARLSEIRNGCERPLTVETAEGPVAAIGEAGLLTFARMKFAP
                     LAGTPHALTARLDGQASLPLPGATPWRFIMVAENAARLLEQNDLLLNLNEPSRIADLS
                     WIRPGKVIRETSLTTAGGKACVDFCKTMNLQFVEFDAGWYGHEYDDAADARGVNLDPK
                     RSKGPLDLPEVIRYAKARDVGVILYVNRRELERRLDELLPLYASWGVVGMKYGFVQVG
                     SQTWTTWLHEAIRKAGEARIMIDIHDEYRLTGNQRTWPNVMTVEGIRGNEEMPDAAHN
                     CALPFTRYLCGPGDYTPCWYNNRVKNTRAHQLALAAVTFSPWQFLFWYDRPSMYQAEP
                     ELDFWRQIPTVWDDTRVLHARIGAYASIARRSGETWFIGSINALERRSLDLPLSFLSP
                     NVRYTAHVYSDAAPDGSDRTRVACATRIVTSADTLTADMAENGGHAVRLAPVAEQPVC
                     PAIREALRGSLDSHEVAGAVSLVLTADRTLHFDACGHADLAAKKPMTPDAIFWIASMT
                     KPVIASAILMLQDEGKLSIDAPAATHLPELANLKTADGRGFSPTVKHLLTHTSGLSEP
                     TQAEAFAARTLAELMPHIVSKPLRFEPGTKWQYSQSGINALGRIVEVVSQQSLPAFLK
                     ARLFDPLGMRDTTFYPDAAQCARIAKAYRQVDGRLEEADMRALYDFRRGPDRYPAANG
                     GLYSTAADYGRFCRMLLKRGTAGDRRILSTEAVAAFSSLQSGELKTGFTDGMGWSLGC
                     GLVRQPQGVTAKLSPGTFGHGGAYGTQAWIDPVRGAAYILMVQRADFPNADASEIRRA
                     FQDAASAIVP"
     gene            14985..>15519
                     /locus_tag="PK576_05955"
     CDS             14985..>15519
                     /locus_tag="PK576_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61173.1"
                     /translation="MARNGVRAFSLPGNRGRKRKMKRKRAAYAYATIASAAALAASAA
                     TLDVPRLPEPAFADREASAAHALPAGRKDGLRTFRLELSFGATPSNNVQAAFGRDGVP
                     ADGALDAGETDLIIGWDRGEWFIAPRGLRGRHAAPAATSNGLQRLTALIRVTGAGVCL
                     PPSFADRDGAVVFAGLPA"
BASE COUNT         3117 a         4485 c         5199 g         2718 t
ORIGIN      
        1 gagtaccggt ctccaaaacc gggtgttggg ggttcgagtc cctcctggcc tgccatgtac
       61 cgctgcggca gaggacgcgg tccagctgaa aaaagctgcg gggttttgtg tcctgaagga
      121 aggcggattg aacgaggttc ctgaaacatg agtaaggtga aagagagcat ccggagtttt
      181 gtcgcgtatc tcggcgaagt cggggtcgag ttcaaaaaga tcacgtggcc tgatcgtcag
      241 gagctggtgg actcaaccgt cgtggtgctc gtgttcatcg tgattctggc cgtggtcgtg
      301 ctgtgctgtg acaagacgat cctgtttttc ctgcagctca tccacgcata atctggaggg
      361 ggccatgacg aagcaatggt ttgtgctgca cacgctgacc ggccaggagc agaaggtcaa
      421 acggtcgatc gaggcacgtg tgcggatcga agagatgggg gagtatatcg gcgaggtgaa
      481 gatccccacc gagaaagttt ccgaggtgaa gaacggggtg aagaagaccg tgacgcgcaa
      541 gttcttcccc ggatatgtgc tgattaatct ggcgctgtat gatgaggcgc gcaatgtgat
      601 cgagcccacc tggcggtttc ttaaggagac ggggggggtg atcgggttca tcggcggttc
      661 gcgtccgttg ccgctctccc cggatgaggt ggactccatt gtcaatcagg cggcggagaa
      721 gaaggaaaag gttgtgccca aggttcagtt cgagccgggc gaggaggtta agatcaccga
      781 cgggccgttc atgagtttta gcggcacggt cgatgaggtg gatcctgacc ggggcaagct
      841 gaaggtctct gtggcgatct tcgggcgtac ggctccggtc gagctggaat actggcaggt
      901 cgagcgcgcg gtcagcgacc gcgcgtaagg cggcttgcgg gaaccggcgc gcgcggcgcg
      961 ccggcggctc gtcaactgcg gcccgttcgc accggtgggg atgccgggag ggccgcgaca
     1021 gacacgaaac agggtaagta tggcaaagaa aatcacgggt accgtgaaac tgcagattcc
     1081 cgcaggcgcg gcgaatcccg caccgccggt gggccccgcg ttgggttccc aaggcatcaa
     1141 catcatggcg ttctgcaaag agttcaacgc caaaacccag gacaaggccg gcctgatcat
     1201 cccggtggtg atcacggtgt atgccgaccg cagtttttct ctggtgttca agagtccccc
     1261 ggcatccgtg ctgctcaaga aggaggcggg tctggccaaa ggttcgggcg tgcccaaccg
     1321 cgacaaggtc gggaaggtga cgcgcgagca ggtccgcaag atcgcggcct tgaaaaaggc
     1381 ggatctgaac gcctccagcg aggaggcggc gatgcggatg atcgaaggca ccgcgcggaa
     1441 catgggcatt gaggtagtgg actgagaagg ggggacgcga aacatggcca aacatggtaa
     1501 acggtatgtc gatgccctga aaaaagcccc ccaggggccg gtgagtctgg aggaggcggt
     1561 ggccttcgtc aaggcgaacc cgggtgcgaa atttgatgag acggtggaag tcgcgatgcg
     1621 cttgggcgtg gatatccgga agtcggatca gacggtccgc ggcacggtga cgctgccgca
     1681 cggttcaggc aaaaaggtcc gcgtggtcgt tttcgccgcg ggcaatcacg ctgatgaggc
     1741 gaaggccgca ggtgcggacg aggtcggcta cgaggatctg atcgagaagg tcaagaacgg
     1801 ctggacggac ttcgatgtgg cgatcgccac gacggacgcc atgaaagagg tgcgtaagat
     1861 cgcccgcgtg ctgggcccgc gcggcctgat gcccaacccg aagaccggca cggtgacgga
     1921 cgataccgcg gcggccgtga aggcggccaa aggcggcaag gtcgatttcc gcatggaccg
     1981 caccgggaac gtttgcgtgc tttgcggcaa gcgcagtttt gacgctgaga agctggttga
     2041 gaacatcaag gcggtggtgg atgcggtccg ggccgaacgt ccggcaggcg ccaaaggcac
     2101 cttcatccgg tcactgacgg taagcgccac catgggcgtg ggcgtgcgtg tgctggtcaa
     2161 ggaagccgcg gaataaggag gcggaggcat gagaaccgaa aaagtttcca ttctgaacga
     2221 ggtgatcgag cgtgtcaaag actcggattt ctgcttcgtt ctgaattatg gcgggctgag
     2281 cgtggcgcag ttgactgcgt tgcgcaagga gctggcgaaa cacgacgcgc gcgcgatggt
     2341 ggtgaaaaac acctatctgg gccgcgtcgc gaaagagcag gggtgggcgg atgtttccgg
     2401 catgttgacc ggcccgaccg cgatcatcac gggacggggc gatgtggccg aggtggccaa
     2461 ggcgctcgtc gagttcgtga agaaaaacga caaggcggcg atcaaaggtg cgaacctcga
     2521 tgccgccatg ctgtcggccg cggatgtgga ggcgctttcc aaactgccgt cgaaggatgc
     2581 gatgcgcggc atattgctgg gcaccctcca ggctccggcc accagccttg tgcgcgtgct
     2641 caacgcgccg ctgcttaacg tcctgtatgc cctcaaggca tatgaggaga agaagaacgg
     2701 atctgcggca taaaccgtgt cgtaaaaacc gcgggatggg agggcggcgg gtgccggctc
     2761 tcccgggtgc ggatacgggc cctgccgctt gtgaagcggt gatgcccgcg ggaaccccgc
     2821 acgcggcaga acgcgtgcgg ttgcacaaaa aaaaggaata caaccatgac gaacgaagaa
     2881 gtgattgatt ttctgagtaa cctctcggtg ctcgaaatct cgaaactggt caaggcgctg
     2941 gaagagaagt ggggcgtgac cgctgccgcg ccggtggccg ttgctgcggc acctgccgcg
     3001 gccgccgctc cggtggaaga gaagaccgag tttgatgtgg tgctggttga ggccggcgcg
     3061 aacaagatca gcgtgatcaa agaggtgcgc gccatcacgg gccttgggtt gaaggacgcc
     3121 aaggacttgg tcgaggccgc cccgaaaccg gtcaaggagg gtgtggccaa ggaagaggcc
     3181 gccaagctga aggaacagct tgagaaggcc ggcgccaagg tcgaggtgaa gtaaccgctt
     3241 cgtcgccacc gcttaacgaa cggggccgtc cgcatgatga cccggacggt cccgtttccc
     3301 ggcgaccggc ctctgcgccg cgaaccgggg aatgcccgta ccgtcatcaa accgcaacgt
     3361 tgcttgaatt gagaagaaac gcacctgaat ctgcctgaat cgcgtgcagc gcaaccgaag
     3421 tgtgaatcag ttaatcaagt ggctgcttac cacacttcca ttcataaccg gttgccatcc
     3481 gtcaccggca ggcatctcag cgggcgggcg gattttccgc cgtgcggatc tcgatccggc
     3541 gacgcgctta ttcatatcca accttcgagg taggcaatgg ttgaccgtaa agtctttggc
     3601 aagctgaaac ccgtcatgca gccgccggat ctgatcgcaa ttcagaccgg gccgtatcag
     3661 gacttcctgc aacgggatgt cgcgcctctc aaacgcgaga acaaggggct tcaggccatt
     3721 ttccatgagg tgttcccgat cgacagtttc gacgggcggt acacgcttga tttcgtcaaa
     3781 tacgacttgg gtgagccgaa gatggatgcg ctcacggctt tggtcgaggg caacacctac
     3841 gccgcgccgc tgcacgcgac cttccgtctc aaggacggcg acgagattcg cgaagaggat
     3901 gtctacatgg gcgagatccc gctcatgacg cgcgatggcg cattcgtcat caatggtgcc
     3961 gagcgtgtga tcgtctcgca gttgcaccgt tctcccggga tctgctccga gcgtacgcag
     4021 catcccaatg ggcagaccct ctattccgtg cggatcatcc ccgaccgcgg ctcctggatc
     4081 gagatccagt tcgattcgag tgacctgatg tgggtctata tggaccgccg ccgccgccgc
     4141 cgcaagtttt tcgtgaccac cttcctccgt accctcggct acgggacgga cgaggatatc
     4201 ctgaaacttt tctacacctt tacgagcctg ccggtgaaca aggagttcac ggatgaggag
     4261 ctcgaagtgc tggtcatgaa ggatgacctg atcgatgtcg aatcccaggc cgtgctggca
     4321 aggcgttacg acccgatcac cccggcgctg ctcgaacaga tcgggaaggc gggtttcagc
     4381 aaaatcgagg tggtcgatgt ctcgttcgac ggcggcgtgc tgatcaagac gttgcgcgag
     4441 gatgccaagg cgaacatcta ttccgaagag gacgccctca aggacatcta caagcggctg
     4501 cgccccggcg atccggccac ggcgtccaac gccaaagccc tgctcaagcg cctctttttc
     4561 gagccccgcc ggtatgacct cggcaaggtc gggcggcaca agatcaacca gcgcctcggg
     4621 ctgtccggca aggttcccga caccttgcgc acactggatg agagcggcgt ggacgtggtt
     4681 gaggcggtcc ggctcctgct gaagatctac accggggaag aaacggtgga tgatatcgac
     4741 catctgggca gccgccgcgt ccgtacggcc ggcgagttgc tggagaacca gtgccgcgtg
     4801 ggtctggccc gcacagagcg gctgatccgc gagcgcatga cgctcttcga tcccgccagc
     4861 ggggtgctgg cgccggcccg tctggtgaac agcaaatcgc tctcggccgt ggttcaggat
     4921 ttcttcggac gcagccagtt gagccagttc atggaccaga ccaatccgtt gagcggcctg
     4981 gcccacaagc gccgcctctc ggccctcggc ccggggggtc tcagccgcga gcgcgccggg
     5041 ttcgatgtcc gcgacgtgca cgcgtcccac tatgggcgca tctgcccgat cgagacgccg
     5101 gaagggccga acatcggtct gatctcttcg ctgtgcatct actcgaagat caacgagtac
     5161 gggtttatcg agagccctta tcgcgtggtc cgtaacggca aggtcacgaa ggaggtgcag
     5221 tacctctccg cggacaagga agaggagttc gttatcgcgc aggccaattc ggagttgaag
     5281 tccgatggct cgtttgtgtc gcagcgtgtg acgtgccgct acaagacgga gtttgtggtg
     5341 gtggaccgct ccagcgtgca gtacatggat gtgtcgccca tgcaggtgat ttcgatcgcc
     5401 gcgggcctga ttccctttct ggagcatgat gacgcgaacc gtgcgctgat gggtgccaac
     5461 atgatgcgtc aggccgtgcc gctgttgcag tcggaaagcc cctttgtggc caccggcctc
     5521 gaggcgcgcg cggcttcgga ttcgtgcgtg acggtgttgg caacggaaga cggtgtggtg
     5581 gcatacgcct cggccacgga gatcatcgtg acgccggacg gcaagatgcc gcccaaaacc
     5641 aagaagggcg aagccatcaa agaggcgggc cgttattcgc tgcaaaagtt ccgccgctgc
     5701 aacgccggaa cctgcttcaa ccagaagccg gtcgtcgagg tcgggcagaa aatccggaaa
     5761 ggcgatgtct tggccgatgg tcccgcgacg tatggcggcg aactggcctt gggcaagaat
     5821 cttctggtgg ccttcatgcc gtggaccggt tacaactttg aagacgccat cttggtgagc
     5881 gagcggatcg tccgcgagga tatcttcacc tcgatccata tccaggagtt cgaggtgggc
     5941 gcgcgcgata ccaaactcgg ccctgaggag atcacccgcg acatcccgaa tgtgggggag
     6001 gaggcgctca agaacctcgg tcccgatggg gtggtgcgaa tcggtgcgga ggtgcggccc
     6061 ggggatatcc tcgtcggcaa aatcacgccc aagagcgaaa ccgagctggc gccggaagag
     6121 cgcctgctgc gcgcgatttt cggcgaaaag gcggcggatg tgcgtgacac gtccctgacc
     6181 gtcccgagcg gggtctacgg catcgtgatg gatgtcaagg tcacctcgca gcaggatgcg
     6241 caccgcccct tcctctcgtt gacggcgtcg tcggaagaga agagggaggt ccgcgatatc
     6301 gaggaagagc gcaaaaagaa gcaggccgcg ctggaggagg agctgaccac cgcgctgagc
     6361 aacatcctgt tgggcgagaa aatcccgctg gacgtgctgg atgtcgagaa cggcgagatc
     6421 atcattcccg ccaaccgcaa aatcaccaaa accctgctcg cgcggctcgc caaggcgcat
     6481 gaccatatcc agatcgagaa cagcccggtc cagcagaaga tcgaagagat catcaacggg
     6541 ttccgcccga aattcgcgga tctggccgaa cagagcagca acgatttcgg ggctgaactg
     6601 gacggcatca tcgatgaggg cggggtgatc aagcaggtgc gcgtgtacat cgccgagaaa
     6661 aagaacctct cggtgggtga caagctcgcc ggccgccacg ggaacaaagg cgttgtcgcc
     6721 aagatcttgc cggattgcga catgcccttc ctgccggacg gcacaccggt cgatatcgtg
     6781 ctcaatccgc tcggcgtgcc ttcgcgcatg aacctcggcc agttgttcga gacctatctc
     6841 ggcgtggcct gcaaagttct gggcttccat gcatcgagcc cggtttttga cggcgtgagc
     6901 gaagaggaga tcgatgcgtt gctgcgccag gcccgcaagg tgcaggagag cgagatcggc
     6961 ggatcggcct ggatctgcga ggatggcaag acggtcctgt atgacggccg cacgggcgag
     7021 ccgttcgatc agcgcgtggt ggtggggcat atctacatgc tgaaactgca ccatctggtt
     7081 accgacaaga tccacgcgcg cgccaccggg ccgtactcgc tggtgacgca gcagccgttg
     7141 ggcggcaaag cccagtacgg cggtcagcgt atgggcgaaa tggaggtctg ggcgctcgag
     7201 gcctacggcg tggcgtacgc gttgcaggaa ctgttgaccg tcaaatccga cgacgtcagc
     7261 gggcgcacac gcatttacga ggcgatcgtg aaaggacaaa acacgctgga tgccggaatg
     7321 ccggagtcgt tctcggtgct gatgcacgag ttgcgtggac tgtgcctcga catgaaactg
     7381 ttgggcagtg ataccgctgg cccgaccccc gaactttcga gaatctaagg cgagacgagg
     7441 agacgcacaa tgaatcctta tgcatcacga gaaatgattg gcgggaccta cgatgcgaat
     7501 gcgcactatg atgccgtaag catcacgctg gcgtcaccgg aaacgatccg gtcgtggagt
     7561 cacggcgaag tccgcaaccc ggaaaccatc aactaccgga cctaccggcc ggagcgtgac
     7621 ggtctgttct gcgagcggat tttcggaccc acccgcgact gggagtgcaa ttgcggcaaa
     7681 tacaagcgca tcaagcacaa gggcgtggtt tgcgaccgat gcggtgtgga agtcacggtg
     7741 tcgcgcgtgc gccgtcagcg catgggccac atcgagctgg ccgtgccggt gtcgcacatc
     7801 tggttcttca aatgcagccc cagccggatc ggcctgattc tggacaaaac cgccaacgag
     7861 ttggaacggg tcctgtatta cgaagattac atggtcatcg atccgggcga gaagagcggt
     7921 ctggagaagg cgcagttaat cacggaatcc gagtatcacg actacgtgga aaaatacggc
     7981 gagagcttca ccgcgatgat gggcgcggag gcggtgcggc gtgtgctcaa cgatgtggac
     8041 ctcgacctgc tgatgcagac gttggaggag cagatcgaga acacccgcag caagcagacg
     8101 cgcaaaaaga tcaccaagcg catgaagatt gtggaaggct tccgcaccag cggcgcgcgg
     8161 ccggaatgga tgatcctcga ctgtctgccg gtcatcccgc cggagttgcg tccgctcgtg
     8221 ccgctggagg gtggacgctt cgcgacatcg gacctcaatg atctgtaccg ccgtgtgatc
     8281 aaccggaata accgcctgaa aaacctgctg tcgctcaaga cgccggatgt gatcatccgt
     8341 aacgagaagc gcatgctgca ggaggcggtg gacgcggtgt tcgataacgg ccgtcacggc
     8401 cgcgcggttg cgggcgcggg gaaccgtccg ctcaaatccc tcagcgacat gctgaagggc
     8461 aagaccggcc ggttccgtca gaacctgctc ggcaagcgcg tggactattc cggccgctcg
     8521 gtgatcgtcg tcggccccga actgaagatc actcagtgcg ggttgcccaa aaagatggcg
     8581 ttgaccctgt tcgagccgtt catcatccgc cgcctcaagg agaagggcat ctgccacacc
     8641 gtgcgcacgg cgcgcaagat gatcgagcgc catgacgagc aggtctggga tatcctggag
     8701 gaggtgacca aaggcaagac ggtgatgctc aaccgtgcgc ccacactgca ccgcctctcc
     8761 attcagagtt tcgagcccgt gctcatcgaa ggcgaggcga tccgcatcca tcccatggtc
     8821 tgcacggcgt tcaacgcgga ctttgacggc gaccagatgg ccgttcacgt gccgctgtcg
     8881 gtcgaggcgc agctggagag ccgcctgctg atgctgacgt caaactcgat cttctcgccc
     8941 gcttccggca agtcgatcat gacgcccaca caggacatcg ccctggggat ctactacttg
     9001 acgattccca gccagagcga caagctcgcg aacaaaatcg ccggcaaagt gccggccggg
     9061 gcagagcggt tgccgctctt caacactacg gcagaagtcg attccgcatt ggcggagggg
     9121 gcgatcacgt atcattcccg catccggttg cgcaatcccg atttcggaac gcaggatcgt
     9181 cgcttcggta atcccgagcc gagcgtgatc gaaaccacgg ccggccgcgt gattttcaac
     9241 gaggtctggc cggacgaact cggtttcgtg aacgataagg tcgataagaa caaactgggg
     9301 gacctgattc tccactgtca tcaggttgcg gggcacgccc gcacggtcaa agcgctggat
     9361 gaactcaaga ccctcgggtt caagcacgcc tgtctggccg gtgtctccat ggggctgaaa
     9421 gatatgatcc gtccgaagaa taaggatgag atcatcgccc aagcccacag cgaggtcgaa
     9481 aaggtggagg cgcaattcca gaccggtgtc atcaccgacg gggaacgcca caacaagatc
     9541 atcgacatct ggaccgccgc caccgaaaag atttcggacg agctgtacaa ggcgatcgac
     9601 cgcaacgtga ccgaaagcgt gtccgagccg aacgagacca accccgtgtt catgatggtg
     9661 gactccaagg cccgcggctc caaactgcaa atccgccagc tcgcgggcat gcgcggcttg
     9721 atggccaacc cgtccggcga gatcatcgaa cgccccatca cggcttcgtt ccgtgaaggg
     9781 ctgagtgtgt tggagtactt catctccacg cacggcgcgc ggaaagggtt ggccgacacc
     9841 gcgctcaaga cggctgacgc cgggtatctg acccgcaagc tggtcgatgt gtcgcaggat
     9901 gtgatcatca cgattcagga ttgcaacacg gtgaacggca tcgaggtcga agcggtggag
     9961 gagggggacg agatgctgat tccgctgcgg acccgcgtgc tcgggcgcac ggcgttgtac
    10021 gatatccgcc atccggccac catggaactg ctggttgcgg cgaacgccga aatcgacgag
    10081 gccgcctgca aggctctgga ggatgccggc atcgaacgcg tgtggatccg cagcgggctg
    10141 acgtgcgacg cgccccacgg gatgtgcgcg aaatgctacg gccgcgatct ctcctccggt
    10201 aaactcgtgg agatcggcac tgcggtgggc atcatcgcgg cccagtcgat cggtgagccc
    10261 ggaacgcagc ttacgatgcg gaccttccac atcggcggca cggcctcgac ggtttccaag
    10321 attccggaaa tcgtgctgaa gaacgccggg ttcatcaagt atgtggatct gcgcatcgta
    10381 cagaacgacg agggcaacac cgtcgtgctg aacaaaaacg gaacgatcga gatcgtggac
    10441 gcgaacggca acgagatcga cacctacaat ctgcagatgg gcacggtgtt gttggccgcc
    10501 gacggcgcgg cggtcaaggc aggccagcgt gtggccgtgt gggatcccca ttcggtgccg
    10561 atcctggcgg ataagcacgg cgtggtgtcg ttccaggatt tcgtggacga tgtgtcggtc
    10621 cgcagcgatc aggatgccga aaccggcgcc acgacactgt tggtgctcga caccaaggaa
    10681 gatttgcacc cgcagatcat tatcaccgat gccaaaaccg gtgagattct gggtttctac
    10741 cacattccgg tcggtgctgt cgtgatggtg aaagagggca cgaaagtctc gcccggcatg
    10801 ctgctcgcga aaacgccgcg taaggaggcg aaaacgcgcg acatcaccgg cggtctgccg
    10861 cgtgtggccg aactgttcga ggcccgccag cccaaggatg cggccgagat ctcccggatc
    10921 gagggcgtgg tcgatttcgg cgagcaccag cgcggcaaac gctgcctgat cgtgcgcgac
    10981 gagggcggcc acgaccagga acacctgatc ccgatgggca aacaggttgt cgtgttcaag
    11041 ggcgaccggg tcaagaaggg ccagcagctt accgagggtc cggtggtgcc gcaggagatt
    11101 ctggatgtct gcggcccgca ggaactgcaa cggtatctgg tcaacgaggt gcagcaggtc
    11161 taccgcctgc agggcgtgga aatcaacgac aagcacatcg agatcatcgt gcggcagatg
    11221 ctgcgtaagg tccgcatcac caatccgggc gacacggact tcctctgggg cgagcaggtt
    11281 tcgcgtcaga agttccttga ggtcaacgag caggtgttgg cttccggtaa gcgcgcggca
    11341 gaggcgcagc ccgccctgct gggcatcacc aaggcttcgc tcgagacgga cagcttcatt
    11401 tcggcggcct cgttccagga tacgacgcgt gtgctgaccg atgcggccac catgggccgt
    11461 gtcgatgagt tgcgcgggtt caaggaaaat gtgatccttg ggcacctgat tccgggaggg
    11521 accggcttcc cgctgcaccg ccacatcaag ctcgtgccgc tggccgatcc gatcagcgag
    11581 gaagagatgg ccgcgctgca ggcggacgcg caggcgcagt tcgacgaagt gttcaactga
    11641 ccgctgcggt cagaccggat ggcgggcggg acgcggcgcg tcccgcccgt cgtttgtttt
    11701 tccggtttac gggacgattg cggacgccgc gtcctgaaag gcgcgacgga tttcggaggc
    11761 gtcggcgttg gggaagtcgg cgcgttgcac catcaggatg taggcggccc cgcggaccgg
    11821 atcaatccat gcctgggtgc cgtacgcgcc gccgtgtccg aaggtgccgg gggagagttt
    11881 cgccgtcacg ccctgcggct ggcgcacaag accgcagccg aggctccacc ccatgccgtc
    11941 ggtgaaaccg gttttcaact cgccgctttg cagcgaggag aaggcggcaa ccgcttcggt
    12001 tgacaggatc cgccggtctc cggcggtgcc gcgcttgagc agcatccggc agaaacgccc
    12061 gtaatcggcg gccgtcgagt agaggccgcc attggccgca gggtagcggt ccgggccgcg
    12121 ccggaagtca tacagtgccc gcatatcggc ttcctccagc cgcccgtcga cctgccggta
    12181 cgctttcgca atgcgtgcgc actgggcggc atccggataa aaggtggtgt cgcgcatgcc
    12241 gagcgggtcg aagagacggg ccttcagaaa agcgggcagg ctttgttgcg agacgacctc
    12301 cacaatgcgg ccgagcgcgt tgatgcccga ctggctgtac tgccatttcg tgcccggctc
    12361 gaagcgcaga ggtttggaga cgatatgcgg catcagttcc gccagcgtgc gcgccgcaaa
    12421 cgcttccgct tgggtgggtt cggacaggcc cgaagtgtgc gtgagcaggt gtttcacggt
    12481 cggcgagaaa cccctgccgt ccgcggtttt caggttggcc agttcaggca ggtgggtcgc
    12541 ggccggcgcg tcgatcgaaa gcttgccctc gtcctgcaac atcaggatcg cgctggcgat
    12601 caccggtttg gtcatggagg cgatccagaa aatcgcatcc ggggtcatcg gttttttcgc
    12661 ggccagatca gcgtggccgc aggcatcgaa gtgaagagtc cggtctgccg tcagaacgag
    12721 cgacaccgcg cccgccacct catgactgtc gagactgccg cgtagcgcct ccctaatggc
    12781 ggggcacacg ggttgttctg ccaccggcgc cagccgcacc gcgtgacctc cgttttcggc
    12841 catatccgcc gtgagcgtgt cggcggaggt cacgatccgt gtggcgcagg ccacgcgtgt
    12901 acggtccgaa ccgtccggtg cggcgtcgct gtatacgtgc gcggtgtagc gcacgttggg
    12961 ggagaggaac gaaagcggga ggtccaggct gcgccgctca agcgcattga tcgagccgat
    13021 gaaccaagtc tcgccgctgc ggcgggcgat ggaggcgtag gcgccgatcc gggcatgaag
    13081 cacgcgggtg tcgtcccaca cggtggggat ctgacgccaa aaatcgagtt cgggttcggc
    13141 ctgatacatc gaaggcctgt cataccagaa gaggaactgc caggggctga aggtgaccgc
    13201 cgcgagcgcg agttggtggg cgcgggtgtt tttcacgcgg ttgttgtacc agcagggcgt
    13261 gtagtcgccg ggaccgcaca ggtagcgcgt gaagggcagg gcacagttgt gcgccgcgtc
    13321 cggcatctcc tcgttgcccc ggatgccttc gaccgtcatg acgttcggcc aggtgcgctg
    13381 gttgccggtg aggcggtatt cgtcgtggat gtcgatcatg atgcgggcct cgcccgcctt
    13441 gcggatggcc tcgtgcagcc aggtcgtcca ggtctggctg ccaacctgga cgaacccgta
    13501 tttcatcccg acgacccccc aagaggcgta aagcggcagc agctcgtcga ggcggcgctc
    13561 gagttcgcgg cggttgacat ataggatgac acccacgtcg cgggccttgg cataacggat
    13621 gacttcgggc agatccagcg ggcccttgga acgtttgggg tcgaggttga cgccgcgcgc
    13681 gtccgcagcg tcatcgtact cgtgaccgta ccagcccgcg tcgaactcaa cgaactggag
    13741 gttcatcgtt ttgcagaaat cgacgcaggc tttcccgcct gcggtggtga gcgaggtctc
    13801 gcggatcacc ttgcccggcc ggatccagga aaggtcggcg atgcgggagg gttcgttcag
    13861 gttcagcagc aggtcgtttt gttccagcag gcgcgcggca ttctccgcca ccatgatgaa
    13921 gcgccagggc gtcgcgccgg gcagcggcaa cgacgcctga ccgtcaagcc gcgcggtgag
    13981 cgcatgcggc gtgccggcca gcggcgcgaa cttcatgcgc gcgaaggtga ggaggcccgc
    14041 ctcaccgatc gcggccacgg gaccttcagc cgtctccacg gtcagcggcc gctcgcaccc
    14101 gttccggatt tcgctcaggc gcgcgggctt gtacgccgcc tgggcagagt aaaccggcca
    14161 agcgcgatga tccccggtaa aaatgaattc ggtgttttcc tttgcgaaaa ggagttgtgt
    14221 gagtgactgt tgcggaaaca catagcggaa ggccagcccg gcgtcatagg cgcggcactc
    14281 gagatgcaga gtcaggtcgt tcgcagcgcg gtttcggaga atgagcgtca gcgcgttata
    14341 ggcgtcggtt acctgtgcgc gctcgccgaa ggggtttttc caagtctggt gtatggtggc
    14401 gcgcttcgcc gaaaccaagg catacccgag agtgaggtcc gtgcggtcaa cagtggtgaa
    14461 gcccagcttg gaaggcagaa tcaccgggac gccgccgaag gtcacgcgat acgtgagagt
    14521 tccctccggg gagagcgcga acacggcttg cagcttttgc ccgggcgact ccagagcgag
    14581 tccctcggcc cgcacaagcg cggcagtgaa aacaacggcg gagagcgcga tgacggcgcg
    14641 aagatatcgg atcatttcct tcctctcttt ccaaacggtt tggcagcaga ataatggatt
    14701 caggagccaa agggaataag caataaaaaa cggttgcaaa gttcccgcaa aaaggagtat
    14761 gtttatcccg atgattggct ggattgattg attgaaccga caaggacaac gcatgaggaa
    14821 aagaccggac cccacagact gatttgattt ttgcgctttc gcgcaaaacg ctccctacga
    14881 aaagccgtca aatctttggg aggagcggga tgcacgggga cgcgcccgat cgggcgcggc
    14941 ctcgtcgccg ggcttctccc aaggtgcttg acggtacctc gtaggtggca cggaacgggg
    15001 tccgcgcctt ttccctgccg ggaaaccggg ggaggaaacg gaaaatgaaa cggaaacgcg
    15061 ccgcatacgc atacgcgacg atcgcctcgg cggcagcgct ggcggcatcc gccgccacgc
    15121 tggacgtgcc gcgcctgccg gagcccgcgt tcgccgaccg cgaggcgtcg gcggcgcacg
    15181 ccctgcccgc gggccggaag gacggtctcc gcacgttccg gctcgagctg tccttcgggg
    15241 ccacgccctc caacaacgtg caggcggcgt tcgggcggga cggcgtcccg gccgacggcg
    15301 cgctcgacgc gggggagacg gacctcatca tcgggtggga ccggggggag tggttcatcg
    15361 cgccgcgcgg gctgaggggg cggcacgccg cgcccgccgc cacgtccaac ggcctccaga
    15421 ggctcaccgc cctcatccgc gtcaccggcg cgggcgtctg cctgcccccg tccttcgccg
    15481 accgcgacgg cgcggtcgtc ttcgcgggcc tccccgccg
//
LOCUS       DAOSLE010000065        15027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4102_length_15027_cov_8.190689, whole genome shotgun sequence.
ACCESSION   DAOSLE010000065 DAOSLE010000000
VERSION     DAOSLE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_15027_cov_8.190689"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2281)
                     /locus_tag="PK576_05960"
     CDS             complement(<1..2281)
                     /locus_tag="PK576_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61174.1"
                     /translation="MTHSSRWLLVSGIGVMACCAMTVLHAQDDLEALLQDLGGQKKKA
                     PAAAVAAPKAEEKPAEPAPAAVAAPAAEEKPAAPAPAAVAAPKAEEKPAEPAPAAAVA
                     APKAEEKPAAPAPAAVAAPAAEEKPAAPAPAAVAAPKAEEKPAEPAPAAVAAPAADDH
                     ASLVSELLTLESIRRKSLDDHGYASLETARKALKEGDYPLAREQYKQALDFIVSRPAT
                     APALEEARQGMAEAFYQEAKQATRKGDYERAMPLAVQAQEHGHPQAPRLIETLKKEPQ
                     KEAMDVSAISHRVNEDSYKGQRDEIRRRLRLSRQFYTTGEYAKALEECELILRDYPFD
                     TEAMELRDRISDRMKTVADVELEATRSLMIRDVRKSWTPERYAIESAQMPRGKSDMTA
                     KRAPGEISSTKTSEQIIQQKLRDIVIPEVTFRPPATIIDAVDFFKQASRDYDKPEIPV
                     EQRGVNLVLKLAPGATAAQAAPAVAEDPFAPAAPAGAASGVPAIPALSARFINLFDAL
                     KLVCDVTGMKFRIRGNIVMIVPVNDPDDELVTRSYNVLATLQERVGAAQSELTQSRGG
                     GDATFMQQADMGGKQDWKTFFQQMGVKWPEGSSISYMATIGKLRVTNTAEELAVFEQV
                     LEDLNVTPRLIEIEARFVEVSQKDLNSLGFEWLLNSDFSFDAGGFLNKALSLKEVSNK
                     QVPLLDSFGQPVFDAAGNQLYAYQPYQAINQTFGTETIPGSIVGYNADGTPIFAGKLG
                     AGQYTMMNAGNHNMGINAID"
     gene            complement(2363..3433)
                     /locus_tag="PK576_05965"
     CDS             complement(2363..3433)
                     /locus_tag="PK576_05965"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOE61175.1"
                     /translation="MAIKLSSFPLLRHYDKLIAIGVLIFLLISLFYLTHVGAARKQAE
                     SQYTRQLESLKPTAAPLAAIEMAEFDDAVRLVRSPLQLDTAETQKAGFLTPERRVVCV
                     IQACKKPIPYAAEVCPFCGGKQPIPPDMDVGLDTDRDGIPDKEEIRLGLNPLDPADAK
                     GDLDGDGFSNLEEFLAGTDPKDPKSHPALVNLLRVKELRGKRLPLIFSGVNKMPDGKL
                     QLVFNQTAPIPRTFWIREGERIGDSDYVAEQVEIKFEDRDNPRMPGLKMRVDVSRVTL
                     LRQSDKKKIVLQINERDKNTDIEAVIVLPLDQTEYTVIENSTLKVREETYRVLTIDAD
                     TTTVIIESQATGQQKVVRKLDS"
     gene            complement(3437..4414)
                     /locus_tag="PK576_05970"
     CDS             complement(3437..4414)
                     /locus_tag="PK576_05970"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOE61176.1"
                     /translation="MKRQQLILIILAVLTVGVCLGAGWFLVSAWMANKEAASVRNADY
                     AAVQKIYRGKVFPDPDTIARVKEDQKALESWLATASNLLHQGDLHIESKSPTGFKQTL
                     QATVRALSARPGAANGRIVAPGFLFGFDQYLGESDSLPAPEHVDRLTGQLTIIERLCN
                     ELCEAGVLEIKAVQRETFDAQTEAGGPDRSQEASSSRRRRRRDEPQAQEAAAPAARKA
                     ASGEFFSKQRFSFEFLARPPAFIGALNRLAAMDLFVVVAEAEFRKTDDPIGRHDTRKK
                     EGAASAADAATKEVDLATIPHAERIVTDPDLDPPVNVRLNIDVYSFEGV"
     gene            complement(4411..6102)
                     /gene="pilM"
                     /locus_tag="PK576_05975"
     CDS             complement(4411..6102)
                     /gene="pilM"
                     /locus_tag="PK576_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOE61177.1"
                     /translation="MFSTDRILALNVGASKLVLAEFSVKSGRPPELLNYGTHVFGVEP
                     ENESETGAQLVPAIREIMRTHGIRNAPLMLSLSGQMVFPRFVRLPAVGEDKLLQMVQY
                     EVEQNVPFPMEEIVWNHQFIGDASTGEQCAMIVAAKIENVRDITDRVVEAGLDPETVD
                     VAPMTLYNCLRYNYAELDGCSMLLDIGARSTNLIFLEDEKIYNRCIPVAGNAITQELA
                     KSFQISFAEAEALKCERAFVSLGGVSASEDEVSERVSKVVRNVVTRLHAEISRSINFY
                     RSQQGGNAPVRIFLTGGSSVMPHLDTFFREKLQVEVLYLNPFTNVAVSNRIDREKAGV
                     DAFVLAETVGLALRRSLACPVEINLMPPEITKRKTLKRRIPFFGLAAAGVFLSLGIWT
                     LYLKQMGKLYAFQGEQVAAKLSVCKAKQASLTQAEKSREAVREKAEAIRDLMWCRTAW
                     LKRVDAVRKSLFEGMWLTELAPVRDAAGTVTGLRIVGRGWADKHKEIEEKAKSAGRTA
                     TAVEELRDRLKTQSVFSGEPQGVRIVGVKDIGAYLIEFTIEATLAASGPKDEAAR"
     gene            complement(6102..6782)
                     /locus_tag="PK576_05980"
     CDS             complement(6102..6782)
                     /locus_tag="PK576_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61178.1"
                     /translation="MNKTVVGRTGMSVMGVAVAMLICTEAAMAQMSRGQTRMGQATAV
                     RTEKREEVNITRMPPPNKTAMVRTPEFNVNVQNTMPKVTRRPREWALFEVKYNSSAKW
                     TDELVFTYHVMTKGKDEEGKEGFSYYTLTVRYIDISKGEHMSCVALPPSQVERHGEPV
                     AVALEITNKEGKVMDSKSESTIPLPKEWWKDSKVLDNAALTRRTGLLDRSKTPFALIN
                     ADDYEVVQ"
     gene            7301..7564
                     /gene="rpsO"
                     /locus_tag="PK576_05985"
     CDS             7301..7564
                     /gene="rpsO"
                     /locus_tag="PK576_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE61179.1"
                     /translation="MSQIDKTAIRKQFQRHDRDTGSSEVQIAVLTKEIEMLTEHLKAN
                     KKDHSSRYGLIRKVNTRRSLLDYLKREDEAVYQKIIKELGLRR"
     gene            7723..9840
                     /gene="pnp"
                     /locus_tag="PK576_05990"
     CDS             7723..9840
                     /gene="pnp"
                     /locus_tag="PK576_05990"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE61180.1"
                     /translation="MKDTTSVSVQVGGATMTIETGLLARQAAGATVCRLGDSLMFSAV
                     TCTDKPREGIDFFPLQVEYREKYYAAGRFPGGFFKRESRPSEKEILTARITDRPIRPL
                     FPEGYRNDVQINNMVLSADTENDTDILSVNASSAALHISDIPFMGPIGCVRVGRIHGE
                     FVINPTHSQRKESDLDLVYAGTRERFLMMEGGAAEITEEDFLAAMKVGHAEVVKIIDA
                     QHELRRLLGKPEKKIEEVAPNAEKMAFLYAEGETALRQALLIVDKLERQNAVNAIKED
                     LQAKALAKWPEEVTAASFVTLFDTLEIDLVRRNILEDGKRIDGRAADEIRKLYAQVGV
                     MPRAHGSAIFERGETSALGSVTLGTKKDAQELDAITGGETSKSFFLHYNFPPYSVGEV
                     GRLGATGRREIGHGALAERSLAQVMPQDYPYSIRVVSDIMGSNGSSSMASICVGSLAL
                     MDAGIPIKAPVAGVSVGLFTNKDESQKILVTDILGAEDHCGDMDFKVAGTRKGITGFQ
                     VDLKLRGLTWDVVEAALARAKQGRLQILDFMESVMPAPRAELSQFAPRITVMTIPVDK
                     IGALIGPGGSNIRRVCEISGTQIDIQDDGSVSIFANNAESLAIAQNEVLALTAEAEEG
                     KIYEGTVTGIKEFGCFVEIMPGKDGLCHISELADRRIGSVEDVCKVGDKMTVKCIGVD
                     DRGRIKLSRREAMRDLDQQKQNG"
     gene            complement(10071..11858)
                     /locus_tag="PK576_05995"
     CDS             complement(10071..11858)
                     /locus_tag="PK576_05995"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020621142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOE61181.1"
                     /translation="MSKTIWCENGFPKVGIRPIIDGRRRGVRESLETQTMNMAKSAAK
                     LISESLTYPDGSPVQCVIADTTIGGCAEAAACANKFERENVGVSLSVTPCWCYGTETM
                     DTAPHTPKAVWGFNGTERPGAVYLAAMLAGYAQRGFPAYGIYGREVQDRTDTTIPDDV
                     KEKLLRFVKAGLAVAMMRGKSYLAVGTSSMGIAGSFIDPDLFQDYLGIRVENVDMCEL
                     DRRAVEGIYDKAEYKKALAWAKKFCKEGKDINKKRLQKSRAAKDADWEYVIKMTLALR
                     DLMVGNPVLAEMGLGEEAVGRNAIAAGFQGQRQWTDYKPNGDFSEAILNSSFDWNGIR
                     APYIVATENDALNGICMLLMYLLTNRAQIFADVRTFWSADAARRVTGKRMTGLNANGF
                     IHLLNSGAACLDATGKQRFKGKPTMKPFWEITAKEAEACLKATRWMPANVEYFRGGGF
                     SSSFLSEGGMPMTMTRISRVKGLGPILQIAEGWSTTLPAGVTETLMARTDPTWPCTWF
                     VPRLTGKGAFRDVYSVMNNWSANHGAISYGHIGADLTTLAAMLRIPVAMSNVPEEQIF
                     RPHAWGLFGIDDPIGADYRACKNFGPIYG"
     gene            12116..14746
                     /locus_tag="PK576_06000"
     CDS             12116..14746
                     /locus_tag="PK576_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOE61182.1"
                     /translation="MKPNCSDLFSFRTAMASLLLAATLSAVARAQAPSPALLLIPQPQ
                     SLRLEASGGAFTLNARTPLSGEAALVEVVRGVLSEHAALDALPSGPGGITVSKPSDPA
                     DALWKNDQAYLLRVLPAGVTIEAAGRDGARYAAQTLVQLLRIAPSVPALTIRDWPAIR
                     TRLAMIATDQGGFQVIDPEYWKRLIRELASYKINAIMPYFDGGTYKYRKYPFLGNKGD
                     DGFTLEKARMLSEYAEQRGVRLIPQQNSLGHLGGVLGHRELQHLRDGGGTINMVSPDS
                     LAFLGDLYDELTQAFPYAHAIHVGGDEFGHDFGKNPQVAARIAEIGKAGVYGAFMTAL
                     RDKLRSRNREMMIWKNEQGLTLEAADRMPRDIAVFDWNYDARQDYPSLDTLLEAGFTQ
                     PWATPAVTRYYSRGNDWDATFANIRNFAAAGIRRRVPGLCTCTWVHGMWGGRNLFELN
                     LYGLVFSAACGWNPPPENETTPFTRAFALQWLGCRDPQAAVWVRDGIHTPYGTRTEQK
                     FWRDNRVQEKYACSPFSTLMESVATTPALEQDARELIAFCDRAETALDTLARSATRNL
                     RTLDYFKHDVRIQRLAANRILTAAELSRWVAGLKIPKPVPERNLLRLTFDSLPTNDAV
                     RVSPTARISGGTLATVPADHWARKGLDIGPLPLPESGVQIEYDVCPLQSGNQYQQFAS
                     TRPSTHHFMAFLGKNRRFSLHTHAGGVWSEQATLTEPCATGTWYRCTVQIRRDNLSFR
                     AVERETGKPVCRSGFVPIDPVGPQLHFSLTDNHGATGNRAPASLWDQVTVSTLKPVPD
                     QPTTPPADLLVRFKRLIQDHNTIEETFRKSVLEAGGGSADTGNLSKGATQFRSRQGRE
                     DTEKLLRMLTAGRLPVNLCE"
     gene            complement(14709..>15027)
                     /locus_tag="PK576_06005"
     CDS             complement(14709..>15027)
                     /locus_tag="PK576_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61183.1"
                     /translation="FLGIARVFLERQGEFLIFMKFHVFHGFIQIGVGVAIAIGIDYLT
                     TPSPPHSLTVFEIAGSARISIRQKPMFTGLCWNSTRSTALFRLNVQKGPLTRTGSPAD
                     GPQ"
BASE COUNT         2875 a         4865 c         4400 g         2887 t
ORIGIN      
        1 cgtcgatcgc gttgatcccc atgttgtggt tgccggcatt catcatcgtg tactggcccg
       61 caccaagctt gcccgcaaaa atgggcgtcc catcggcatt gtacccgaca atggaacccg
      121 gaatcgtttc ggtgccaaag gtctggttga tcgcctgata cggctgatag gcgtaaagct
      181 ggttacctgc agcgtcaaat acaggctgcc cgaacgaatc caacagcggc acctgtttgt
      241 tgctgacctc tttgaggctg agggctttat tgaggaaccc gcccgcgtca aagctgaagt
      301 cactgttgag cagccattca aacccgaggg agttgaggtc cttctgggag acttcgacaa
      361 agcgcgcttc aatctcaatc aggcgcggcg tgacgttgag atcctccagc acctgctcga
      421 acaccgcgag ttcctcggcg gtattggtga cgcgcaactt accaatggta gccatgtagg
      481 agattgagga gccctcgggc catttgacgc ccatctgctg gaagaaggtc ttccagtcct
      541 gcttgccccc catatcggcc tgctgcatga aggtggcgtc tccgccgccg cggctctggg
      601 tcaactcgct ctgggccgcg ccgacgcgtt cctgcagcgt ggccagcacg ttgtaggagc
      661 gggtgaccaa ctcgtcatcc ggatcgttga ccggcacgat catcacgatg ttgccgcgaa
      721 tacggaattt catgcccgtg acatcgcaga ccagtttcag cgcgtcaaaa agattgatga
      781 accgcgcact caacgcgggg atcgcaggca ccccactcgc cgcgcctgcg ggagcggcgg
      841 gcgcgaaggg atcctccgcc accgcagggg cagcctgcgc tgccgtggcg cccggggcca
      901 gcttaagcac caagttgacc ccgcgctgct cgaccggaat ctccggctta tcgtagtcgc
      961 gactcgcctg cttgaagaaa tcgaccgcat caatgatcgt cgcgggagga cggaaggtga
     1021 cttcggggat cacgatatca cgcagtttct gctggatgat ctgctccgac gttttggtgc
     1081 tgctgatctc gcccggcgca cgtttggcgg tcatgtcgct cttgccgcga ggcatctggg
     1141 cgctttcgat cgcgtagcgc tcgggcgtcc agctcttgcg cacatcccgg atcatcagac
     1201 tgcgggtcgc ctccagttcg acatcggcga ccgtcttcat acggtcagag atccggtcgc
     1261 gcagctccat cgcctcagta tcaaaaggat aatcgcgcag aatcagctcg cactcctcta
     1321 aagccttggc atactcgccg gtggtgtaaa actgacgtga caaccggaga cggcggcgaa
     1381 tctcatcacg ctgaccctta taggaatcct cattcacgcg gtgcgagata gccgagacgt
     1441 ccatggcctc tttctgcggc tccttcttca gcgtttcgat cagccgaggc gcctgggggt
     1501 ggccatgttc ctgcgcctga acagccagcg gcatagcccg ctcgtaatcg cccttccggg
     1561 tcgcctgctt cgcttcttgg tagaatgcct cggccatccc ctgccgcgcc tcctccagcg
     1621 ccggagcggt cgccgggcga ctgacgataa aatccaacgc ctgcttatac tgttcgcggg
     1681 ccaacgggta atccccctct ttcagtgcct tacgcgcggt ttccaacgag gcgtacccat
     1741 gatcatccaa tgacttgcgg cggatcgact ccagagtgag cagctcggac accagactcg
     1801 catgatcatc cgccgccggc gcggccacag cggcgggcgc gggctcggcg ggcttctcct
     1861 ccgccttagg cgcggccacg gcagccggtg caggcgcggc gggcttctcc tccgccgcag
     1921 gcgcggccac ggcggcgggc gcgggcgcgg cgggcttctc ctccgcctta ggcgcggcca
     1981 cggcagcggc gggcgcaggc tcggcgggct tctcctccgc cttaggcgcg gccacggcgg
     2041 cgggcgcggg cgcggcgggc ttctcctccg ccgcaggcgc ggccacggcg gcgggcgcgg
     2101 gctcggcggg cttctcctcc gccttaggcg cggctacagc ggcggcaggc gcttttttct
     2161 tctgtccgcc taaatcctgc aggagcgctt cgagatcgtc ctgggcatgc agaaccgtca
     2221 tcgcgcaaca ggccatgact ccgatacccg acaccaacaa ccaccttgaa ctatgcgtca
     2281 tctgggggga ctcctctttg tgtggcagct atccaatcct atctggtaat aaaaatcagg
     2341 cttactatac cgtagacggc gttcacgaat caagcttgcg aacaactttt tgttgtccgg
     2401 tcgcctggct ctcgataatg accgtcgttg tgtccgcgtc gatcgtgagc acacggtaag
     2461 tttcctcacg gaccttcaac gtgctgttct caatcaccgt gtactcggtc tgatcaaggg
     2521 gcagaacaat caccgcctcg atatccgtgt ttttgtcacg ttcattgatt tgcagcacga
     2581 tctttttctt gtcgctctgg cggagcaatg tgacacgtga cacatcgacc cgcattttga
     2641 gacccggcat tcgcggatta tcccgatcct cgaacttgat ttcaacctgt tcggccacat
     2701 agtcactgtc gccgatacgt tcgccttcac ggatccagaa ggtgcgcggg atcggtgcgg
     2761 tctggttgaa caccagctgc agttttccgt ccggcatttt gttcacgcca ctgaagatca
     2821 gcggcagacg cttaccgcgc agttctttga cacgcaacag attgaccagc gccgggtggg
     2881 atttcgggtc tttgggatcc gtgccggcca gaaactcctc gagattcgaa aacccgtcgc
     2941 cgtccagatc gcctttggcg tcagcgggat ccagcgggtt gagcccgagg cggatctcct
     3001 ccttgtcggg aatgccgtcg cggtccgtat ccagccccac gtccatgtcc ggcgggatcg
     3061 gctgtttccc tccgcagaag gggcagacct ccgccgcata ggggatgggt ttcttgcaag
     3121 cctgaatcac acagaccacc cggcgttccg gagtcagaaa tccggctttc tgggtttccg
     3181 cggtatccaa ctggagcgga ctgcgcacca gccgtaccgc gtcatcaaac tccgccatct
     3241 caatagcggc caacggcgcg gcggtgggtt tgaggctttc gagctggcgc gtgtactggg
     3301 actcggcctg tttgcgggcc gcaccgacat gggtgaggta gaaaagagag atcagcagga
     3361 aaatgagcac accgatggca atcagcttgt catagtgccg caacaggggg aagctggaaa
     3421 gttttatggc cacaggttac actccctcaa acgaatacac atcgatattc agcctcacgt
     3481 tgaccggcgg gtcgagatcc gggtcggtta cgatccgctc agcatgcggg atcgtagcga
     3541 ggtccacctc cttggtcgcg gcatcggcgg cagacgcggc cccctctttt ttcctggtgt
     3601 cgtgccgtcc gatggggtca tccgtcttgc gaaattcggc ctccgcaacc accacgaaca
     3661 ggtccatggc cgccaaacgg ttgagcgccc caatgaatgc ggggggacgg gcgagaaact
     3721 cgaacgagaa gcgttgtttg gagaagaact ccccggacgc cgctttccgg gctgcgggcg
     3781 ccgcggcctc ctgcgcctga ggctcgtccc gacgccgacg acgacgcgac gaggaggcct
     3841 cctgcgaccg gtccggacca ccggcctcgg tctgcgcgtc aaaggtttcg cgctgcaccg
     3901 ccttgatctc cagaacacca gcctcgcaca gctcattgca caaccgctcg atgatcgtca
     3961 actgaccggt cagacggtcc acatgctctg gggcgggcag gctgtccgac tcgccgaggt
     4021 attgatcgaa gccgaacagg aacccgggcg cgacgatgcg cccgttggcg gcgccgggcc
     4081 gtgccgacag cgcgcgcacc gtagcctgca gcgtctgctt gaacccggtg ggactcttcg
     4141 actcgatgtg caggtcaccc tgatgcagca ggttggaagc cgtggccagc caagactcca
     4201 gcgccttctg atcctccttt acccgtgcga tggtatccgg atcggggaag accttgcctc
     4261 ggtagatttt ctgcacggcg gcatagtcgg cattgcgcac cgaggccgcc tccttattgg
     4321 ccatccatgc ggaaaccaag aaccaccccg cgccgaggca tacccccacc gtcagcacag
     4381 ccagaatgat caaaatgagt tgttgccgct tcatcgtgcc gcctcatcct tcgggccgga
     4441 cgccgccagc gtcgcctcga ttgtgaattc gatcagatac gccccgatgt ccttgacacc
     4501 cacgatacgc accccctggg gttcaccgct gaaaaccgat tgggttttaa gccggtcacg
     4561 caactcctcg accgccgtgg ccgtgcggcc ggcggatttg gccttctctt cgatctcctt
     4621 gtgcttgtcc gcccagccgc ggccgacgat gcgcagtccg gtgacggtgc ccgccgcatc
     4681 gcgcaccggc gcgagttcgg tcagccacat accctcgaaa agactcttgc gcacagcgtc
     4741 cacacgcttc agccaagcgg tccgacacca catcagatcc cgaatcgcct ctgccttctc
     4801 ccgcacggcc tcgcggcttt tttcggcctg cgtcagcgag gcctgctttg ccttgcacac
     4861 gctcagcttg gccgcgacct gctctccttg gaaggcatag agtttgccca tctgcttgag
     4921 gtagagcgtc cagatgccga gactcaaaaa cacgcccgcc gcagccaaac cgaaaaacgg
     4981 gatgcgccgc ttcagtgtct tgcgctttgt gatttccggc ggcatcaagt taatctcaac
     5041 cgggcacgcc agcgaacggc gcaacgccag ccccacggtc tcggccagca caaatgcgtc
     5101 gacgccagcc ttttcacggt cgatccggtt gctcaccgcc acattcgtga aggggttgag
     5161 gtagagcact tcaacctgaa gcttttcccg gaagaacgtg tcgagatgcg gcatcaccga
     5221 cgaccctccg gtcaggaaga tccggacggg cgcattgccg ccctgctggc tacggtagaa
     5281 attgatcgac cggctgatct ccgcgtgcag gcgggtgacc acattgcgca cgactttgga
     5341 gacgcgttcg gaaacctcgt cctcggaagc cgaaacaccc cccagcgaga caaaggctcg
     5401 ttcgcacttc agggcctcag cctcggcaaa ggagatctga aaacttttag ccagttcttg
     5461 cgtgatggcg ttacccgcca cggggatgca gcggttgtag atcttctcgt cctccaagaa
     5521 gatcaggttg gtggaacgcg caccgatgtc cagcagcatg gagcatccgt ccaactcggc
     5581 atagttgtag cgcagacagt tgtacagggt catgggcgcg acatcgacgg tctccggatc
     5641 gagcccggcc tccaccacgc ggtcggtgat gtcacgcacg ttttcgatct tggccgccac
     5701 gatcatcgcg cactgctcac cggtggaggc gtcgccgatg aactggtggt tccaaacgat
     5761 ttcctccatg gggaagggta cgttctgctc cacctcgtac tgcaccatct gcagcagctt
     5821 atcctcgccg acagcgggga ggcgcacgaa acgggggaac accatctgtc cggagagcga
     5881 caacatcagc ggcgcgttcc ggataccatg cgtccgcatg atctcgcgga tcgccggaac
     5941 cagttgcgca ccggtttcgg actcgttctc cggctcgacg ccgaagacat gggttccgta
     6001 attgagcagt tccggcggcc tcccgctttt gacggagaat tcggccagaa ccaatttgct
     6061 cgcaccgaca ttcagcgcga ggatgcgatc cgtcgagaac actattgcac cacctcgtaa
     6121 tcgtccgcgt tgatcagggc gaacggggtc tttgaacggt caagcaggcc agtgcggcgg
     6181 gttagcgcgg cattgtccag caccttgctg tccttccacc actctttcgg gagggggatc
     6241 gtggattcgg acttgctgtc catcaccttg ccctccttgt tcgtgatctc cagcgccacg
     6301 gccacgggct cgccatgccg ttcgacctga ctcgggggaa gcgcgacaca actcatgtgc
     6361 tcgcctttgg agatgtcaat ataccggacc gtaagggtgt agtagctgaa cccctctttg
     6421 ccttcttcgt ccttgccctt ggtcatgaca tgataggtga agaccaactc gtcggtccac
     6481 ttcgcgctgc tgttgtactt gacttcgaac aacgcccatt cgcgcggccg acgggtgact
     6541 ttcggcatcg tgttctggac attcacgttg aattcggggg tgcgcaccat cgccgtcttg
     6601 ttgggaggag gcatgcgtgt gatgttcacc tcctcgcgct tctccgtccg cacagccgtt
     6661 gcctgaccca tacgcgtctg cccacggctc atctgggcca tagccgcttc cgtacaaatc
     6721 agcatggcca ccgccacgcc catcacgctc atacccgtcc gtccgacaac cgtcttgttc
     6781 atgatcgttc tcccagtcgt gaatccaacc tctccacgca agccgctgtt accacatgta
     6841 gtgcgtacaa tttaatgcgg agtgagacgg aatgcaaccc tgaaacagaa aaagttcttc
     6901 atcgggacat atcccttgtt gccgcaacgg gtgcggacaa gcgaaaacgg gaatgaaaac
     6961 agggggggga acgcgcctgt ttgcccgtcg tccccgtcct ctcgtgtacg tgcacgggca
     7021 ccgcttcgct gtgtacgggc agggtccgaa ggcacaacgg tcaattcggg aatgcgcgat
     7081 gaccagattc tctccgcccc gccgcttgac atcccgggtg cgatggatta cagtgcgccc
     7141 cttcctgcgg tgcgtctcag gagacactcc ccgctgcggg tcatctggcg cttgcgtccg
     7201 gatttcgctt tacacccggc ggggggggac tctatagtgt gcgccggaaa gtaacgacaa
     7261 aaaagaaaga aatcgcggaa caaggaccgt aaagcagact atgagccaga ttgacaagac
     7321 cgctatccgt aagcaattcc aacgccatga ccgggacacc ggatcgagcg aagtccagat
     7381 cgccgttctc accaaagaga tcgagatgct caccgagcac ctcaaggcga acaagaagga
     7441 tcacagctcg cgttatgggc tgatccgtaa ggttaacacc cgccgtagct tgcttgacta
     7501 tctcaagcgc gaagacgagg cggtttacca gaagatcatc aaagagctcg gcctccggcg
     7561 ttaatcgtgc gcgaaggcgg ccgtgcgcct gtcgcgcgcg gccggaagcg cccggaccga
     7621 catcccgggc gcgctttcag acggacctct gcacttgttc gaataggcca tctttcctgg
     7681 ccaaacgctt tccggtcctt gtccgctgaa aggtgacaca cgatgaagga tacaacctct
     7741 gtctccgtcc aagtgggcgg ggccacgatg accattgaga ccggactgct cgcgcgtcag
     7801 gctgccggag ccaccgtctg ccgtctgggt gattccctga tgttctccgc tgtgacctgc
     7861 acggacaagc cgcgcgaggg gatcgacttc ttcccgctgc aagtcgaata ccgcgagaag
     7921 tattacgcgg ccggccggtt ccccggcgga tttttcaaac gcgagtcgcg gccgagcgag
     7981 aaggaaatcc tcacggcacg catcaccgac cgccccatcc ggccgctctt cccggagggc
     8041 tatcggaatg atgtccagat caacaacatg gtgctctcgg cagacaccga gaacgacacc
     8101 gacatccttt ccgtcaacgc ctcctcggct gccctccata tctctgatat ccccttcatg
     8161 gggccgatcg gctgtgtgcg tgtgggccgt atccatggcg aattcgtgat caaccccacg
     8221 cactcccagc gcaaggagag tgaccttgac ttggtctacg cgggcacgcg cgagcgtttt
     8281 ctcatgatgg aaggtggcgc cgcggagatt accgaagagg atttcctcgc cgcgatgaag
     8341 gtcggccatg cggaggtcgt caaaatcatc gatgcccagc acgagttgcg ccggttgctc
     8401 ggcaagccgg aaaagaagat cgaagaggtc gcgccgaacg cggagaaaat ggcgttcctc
     8461 tatgccgagg gcgagaccgc tctgcgtcag gccctgctca tcgtcgacaa gctggaacgc
     8521 cagaatgcgg tcaatgcgat caaggaagac ttgcaggcca aggccctcgc aaagtggccg
     8581 gaagaggtca ccgccgcatc cttcgtcacg ctcttcgaca cgctggagat cgacctcgtc
     8641 cgcaggaata tccttgagga cggcaagcgc atcgacggac gcgccgccga tgaaatccgc
     8701 aagctctacg cgcaggttgg cgtcatgccc cgggctcacg gctcggcgat tttcgaacgc
     8761 ggcgagacct ccgccttggg gtcggtcacg ctcggcacca aaaaagacgc tcaggaactc
     8821 gatgccatca ccggcggcga aaccagcaag tctttcttcc ttcactacaa cttcccgccc
     8881 tacagcgtcg gcgaggtcgg caggctcggc gccacgggac gccgcgagat cggccacggc
     8941 gccttggccg agcgttcgct cgcccaagtc atgccgcagg attacccgta cagtatccgc
     9001 gtggtctccg acatcatggg ctcgaacggt tcctcctcca tggcctccat ctgcgttgga
     9061 tcgctggcct tgatggatgc gggcattccg atcaaggctc ccgtcgcggg tgtctcggtc
     9121 ggcttgttca caaataaaga cgaaagccag aagattctgg tgaccgacat cttgggagcg
     9181 gaagaccact gcggcgacat ggacttcaag gtggccggaa cccgcaaggg gatcacgggc
     9241 ttccaagtcg acctgaaact gcgcggcctg acctgggatg tggtagaagc cgctctcgcg
     9301 cgcgccaaac agggacggtt gcagatcctc gacttcatgg agtccgtcat gccggctccg
     9361 cgcgccgaac tctcgcagtt cgcgccgcgc atcacggtca tgacgatccc ggtggataaa
     9421 atcggggcgc tcatcggccc gggcggctcg aacatccgcc gcgtgtgcga gatctccggc
     9481 acccagatcg acattcagga cgacgggtcg gtcagcatct tcgccaacaa cgccgagtcg
     9541 ctggccatcg cgcagaacga ggtcttggcg ctcaccgccg aagcggaaga gggcaagatc
     9601 tacgaaggca ccgtcaccgg catcaaggag ttcggctgct tcgtcgagat catgcccggc
     9661 aaggacggcc tctgccacat cagcgagctc gcggaccgcc gtatcggctc cgtcgaggat
     9721 gtctgcaagg tcggcgacaa gatgacggtc aagtgcatcg gcgtggacga ccgcggccgc
     9781 atcaaactca gccgccgcga agccatgcgc gacctcgacc agcagaaaca gaacggctga
     9841 acgccgcgct catcgtaatc tgaaagcccg cgataatacc catcgcgggc tttttgttgg
     9901 tttctgaccc gcgacactcg acccgcgacg tggcacgggc atcttgcccg tgttgcatgg
     9961 gcgaaacgcc catgccacga atacactccc cgcccgcgtt cccgacaccg caagtcgccc
    10021 gtcgcgggtc aatccctaac tctcggctat ctccgcgcta cgcacggagt tcaaccgtag
    10081 atcggaccga agttcttgca ggcgcggtag tccgcgccga tcggatcgtc gatgccgaac
    10141 agaccccagg cgtgcggccg gaagatctgc tcttcgggca cgttggacat cgcaaccgga
    10201 atgcgcagca tggccgcaag cgtggtcaga tccgcgccga tgtggccgta gctgatcgcg
    10261 ccgtggttgg cgctccagtt gttcatgacg ctgtagacgt cgcggaaagc gcccttgccc
    10321 gtcaggcgcg gcacgaacca cgtgcagggc caggtcggat ccgtgcgcgc catcagcgtc
    10381 tcggtgacgc ctgcgggcag cgtggtggac catccctcgg caatctgcag tatcgggccg
    10441 agccccttga cacggctgat gcgcgtcatg gtcatcggca tgccgccctc ggagaggaag
    10501 ctggaggaga agccgccgcc gcggaagtac tcgacgttgg ccggcatcca gcgcgtcgcc
    10561 ttgagacacg cctccgcctc tttcgcggtg atctcccaga acggcttcat cgtgggcttg
    10621 cccttgaaac gttgcttgcc ggtggcatcg agacaggccg caccggagtt cagcaggtgg
    10681 atgaacccgt tggcattgag gccggtcata cgcttgccgg ttacacgcct agccgcgtcc
    10741 gcgctccaga aggtgcgcac atcggcgaag atctgggcac ggttggtcag cagatacatc
    10801 agcagcatgc agatgccgtt gagcgcgtcg ttttccgtcg ccacaatgta cggcgcacgg
    10861 atgccgttcc agtcgaacga gctgttgagg atcgcttcgg agaagtcgcc gttgggtttg
    10921 tagtcggtcc actggcgctg cccctgaaag cctgccgcaa tggcattacg ccccaccgcc
    10981 tcttcgccga ggcccatctc cgccaacacc ggattgccga ccatcagatc gcgcagggcc
    11041 agcgtcattt tgatgacata ctcccagtcg gcatccttgg cggcgcgcga tttctggagc
    11101 cgcttcttat tgatgtcctt gccttctttg cagaacttct tggcccacgc cagcgccttc
    11161 ttgtactcgg ccttgtcgta gatgccctcc acggcgcgcc ggtccaattc gcacatgtca
    11221 acgttctcaa cgcgaatgcc gaggtagtcc tgaaagagat ccggatcaat aaacgaaccc
    11281 gcgatcccca tcgacgaggt gcccacggca agataggatt tgccgcgcat catcgccact
    11341 gccagcccgg ccttgacgaa gcgcaacagt ttttccttca cgtcgtccgg tatcgtcgtg
    11401 tcggtgcgat cctgcacctc acgcccgtag atcccgtaag ccgggaagcc gcgttgcgca
    11461 taacccgcga gcatcgcggc cagatagacc gcgccgggac gttcagtgcc gttgaagccc
    11521 cagaccgcct taggcgtatg gggtgccgtg tccatcgtct cggtgccgta gcaccagcag
    11581 ggcgtgacgc tcaacgagac ccccacattc tcgcgctcga acttgttcgc gcaagcggcg
    11641 gcctcggcgc agccgccgat cgtggtatcc gcgatcacgc actgcaccgg cgagccgtcg
    11701 ggataggtca gactctcgga aatcagcttg gccgcgctct tggccatgtt catggtttgc
    11761 gtttctaacg actcacgcac cccgcgacgc ctgccgtcaa tgatcgggcg gatgcccact
    11821 ttcggaaaac cgttttcgca ccaaatcgtt ttactcattc gtgaaccctc cttatgaata
    11881 aggaatagtg taccacccga tcctcctttt gactagtccg ctttgcgcaa tttaaaattt
    11941 tacaagggaa ccggttatct gaaagaggga cgggcgagcc ggaaacgcgc gcaccccggc
    12001 cgccaaccga ttgccggttc aagcgttcgc cgggttgccg ggaaccgttt tttttggcag
    12061 actcggggta tgggcgaacc ggttctggtt ccgcccgtcc tgaaaaagga gacacatgaa
    12121 accgaactgt tccgatcttt tttcgttccg taccgcaatg gcatcgctgc tgctggccgc
    12181 aaccctgtcc gccgtggcgc gcgcgcaggc tccctcccct gccctcttat tgatcccgca
    12241 accccaatcg ctgcgactcg aggcctccgg cggggctttc accctgaatg cccgcacacc
    12301 gctctccggc gaggccgctc tggttgaggt ggtgcgcgga gtgctgtcgg aacatgccgc
    12361 ccttgatgcg ctgccgtccg gtcccggcgg catcacggtg agcaagccct ccgacccggc
    12421 tgacgcgctc tggaaaaatg accaggccta tctgttgcgc gtactccccg caggcgtgac
    12481 cattgaggcc gcgggccgcg acggtgcccg gtatgccgcg caaacactgg tccagctgct
    12541 ccgcatcgcg ccgtccgtgc ctgcgctcac gatccgcgac tggcccgcga tccgcacacg
    12601 tctggcgatg atcgcgaccg accaaggagg attccaggtg atcgaccccg aatactggaa
    12661 gcgcctgatc cgcgagctgg cctcttacaa gatcaacgcg atcatgccct attttgatgg
    12721 cggcacgtac aagtaccgca aatacccgtt tctcggcaac aagggcgatg acgggttcac
    12781 gctcgaaaag gcccggatgc tgagcgagta tgcggaacag cgcggtgtgc ggctcatccc
    12841 tcagcagaac tcgctgggcc acctcggcgg cgtgctgggc cacagggagt tgcagcatct
    12901 gcgcgacgga ggcggcacga tcaacatggt cagccccgat tcgctcgcct ttctcggcga
    12961 cctctatgac gaactgacgc aagccttccc gtacgcgcac gcgatccacg tggggggcga
    13021 tgagttcggc cacgatttcg gcaagaaccc gcaggtggcg gcccgtatcg cggagatcgg
    13081 caaagccggg gtgtacggcg cgttcatgac cgccctccgc gacaagctcc gcagccgcaa
    13141 ccgcgagatg atgatctgga agaacgaaca ggggctcacc ctcgaagccg ctgaccggat
    13201 gccgcgcgac atcgcggtgt tcgactggaa ctacgacgcc cgacaggatt atccctcgct
    13261 ggacacgttg ttggaggcgg gcttcaccca accttgggcc accccggccg tgacccgtta
    13321 ttacagcaga gggaacgact gggacgcaac gttcgcgaac atccgcaact tcgcggcggc
    13381 cggtatccgt cggcgtgtgc ccggactctg cacctgcacc tgggtccacg gcatgtgggg
    13441 cggacgcaac ctgttcgaac tgaacctcta cggactggtc ttcagtgccg cttgcggctg
    13501 gaatccgcct cccgaaaacg agaccacccc cttcacccgc gctttcgcgc ttcaatggct
    13561 gggatgccgc gatccgcaag ccgccgtctg ggtccgcgac ggcatccaca cgccatacgg
    13621 cacacgcacg gagcagaaat tctggcgcga taaccgggtg caggaaaaat acgcctgctc
    13681 tcccttctcc acgctgatgg agtcggtcgc cacaacgccc gcgctggagc aagacgcgcg
    13741 tgaactgatc gcgttctgcg atcgtgcgga aacggcgctc gacacgctcg cgcgttccgc
    13801 cacccggaac ctccgcacac tcgattactt caaacacgat gtccggatcc aacgtctggc
    13861 cgcgaaccgc atcctgactg ccgcggaact gtcgcgctgg gtcgcgggcc tgaagattcc
    13921 caagccggtt ccggagcgga atctgttgcg tctcacgttc gactcccttc caacgaacga
    13981 cgccgtgcgg gtcagcccga cagcccgcat ctccggcggc acgctcgcca ccgtgcccgc
    14041 ggatcattgg gcgcggaagg gactcgatat cggtccgctg ccattgcccg aaagcggcgt
    14101 gcagatcgaa tatgacgtgt gcccgctgca atccgggaac cagtatcagc agttcgcctc
    14161 aacccgtccc tcgacgcacc acttcatggc cttcctcgga aaaaaccggc gtttcagcct
    14221 gcacacccac gccggaggcg tctggagcga acaggccacg ctcaccgaac catgcgcgac
    14281 cggaacgtgg tatcgctgca ccgtgcagat ccgacgcgac aacctctcct tccgggccgt
    14341 ggagcgcgag accggcaaac cggtctgccg ctcgggtttc gtgccgatcg atcctgtggg
    14401 gccgcagctc cacttctccc tcacggacaa tcacggcgcc acgggaaacc gcgcgcccgc
    14461 ctcgctgtgg gatcaggtga ccgtctccac gctgaagccc gttccggacc agccgaccac
    14521 cccacccgcc gaccttctgg tccgcttcaa gcggcttatc caggatcaca acacgatcga
    14581 ggagactttc cgcaaatccg tgctggaggc aggcggcggt tcggcggaca ccggcaacct
    14641 cagcaaagga gccacgcagt tccgcagccg ccaaggccgc gaagatacgg agaaactgct
    14701 ccgcatgctc actgcgggcc gtctgccggt gaacctgtgc gagtgagtgg ccctttttga
    14761 acgttcaatc taaaaagagc cgttgaacgg gtagaattcc agcaaagccc cgtaaacatt
    14821 ggcttttgac gtattgagat gcgggcggag cccgcaatct caaagactgt gagactgtga
    14881 ggcggtgagg gtgtggttaa ataatcgatt ccgatagcga tagcgacccc gaccccgatt
    14941 tggatgaaac catgaaatac atgaaacttc atgaaaatca ggaattcccc ctggcgttcc
    15001 aaaaaaaccc gagcgatgcc caaaaaa
//
LOCUS       DAOSLE010000066        14597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4234_length_14597_cov_6.682850, whole genome shotgun sequence.
ACCESSION   DAOSLE010000066 DAOSLE010000000
VERSION     DAOSLE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4234_length_14597_cov_6.682850"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..2289)
                     /locus_tag="PK576_06010"
     CDS             complement(118..2289)
                     /locus_tag="PK576_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61184.1"
                     /translation="MKALVLGVMAFCVVASGWGGSVPSPAVCVRACPDGAQSTPRLWN
                     GIAPQVKAWELGKRQETLAWFEENQFGKTPVGRLPDEVIGETYVAFTNAGVRIEIHCS
                     LPAGADAAHPAPVFLFGDHRGGNAPPDFAQETYEGIPTNSITARGYAYVRWNFNDVCP
                     NVDANHSFGRWPLGIIAYAATGDRNATNVVRQANSWGTIGAWAWGNSRVMDWIETRPE
                     LDASRVAVLGHSRGGKTALWTGAQDERFAMTISNGSGCGGARLGRAKAPKAETIRQIL
                     KNFPNWFCPTFTAWIDRDAELPHDADDLIRLIAPRLVYVASGSEDAWAGPAAEKAAWD
                     AAHDVYRAYKLEERMGYHCHDGPHKLRADDWERFMDFADRQLKAPSTADSDAIERRIA
                     AACVDGSRAVTLTKNAASVDGAWHLTRAILVPDDFTLVFDGCRVELVDGTQDNLVRNA
                     GAVEGRCTPNRGIRILGKNGAVLSGGKRNHYLPRRSGDPNGWRSVGILLCDVSDYEVG
                     GFTMEETQCWAISQERCCRGHIHDIVFGSTDILFNQDGVDVRKGCHDLVIENISGMTG
                     DDVVALTAYRDPPGSPARFGMQIGGNATLGEKDDIYNVTIRNIRARSSGGHGVIRLLT
                     CDGIRMHHITVEDVVDTATGNERRPQATIRIGDKSYHRTRRCELREMHHVDVRGVKAS
                     GKVAVWIRGPLSDSSVVSVTESPGGKKYEISAPMENVRLDP"
     gene            2572..3405
                     /locus_tag="PK576_06015"
     CDS             2572..3405
                     /locus_tag="PK576_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE61185.1"
                     /translation="MQSLLNHLNQLQELVLIRDEHRTTGDGSHMERLNDSIDQLTDKL
                     PPEIKSLYQRLYKKDHIVMAPMYNGCCAICGMRLPISQVQSVRLCKQIQNCPSCARIL
                     FEESGAPRWVGDVPSRTEPRKSGISRFSAESLMIPDLKATNGREAIVELATVMEQQKF
                     VSGAEKLVASALEREAVLSTAMDNGLAFPHVRGVEGGGLTLALGVAKKGIRYDEAGSI
                     VNLVFFITIPTAVSAFYLRLMSGLTETFLKEQNREAMLAAETPELLWKALIKATRYTI
                     K"
     gene            complement(3469..4194)
                     /gene="pflA"
                     /locus_tag="PK576_06020"
     CDS             complement(3469..4194)
                     /gene="pflA"
                     /locus_tag="PK576_06020"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOE61186.1"
                     /translation="MNPQVTGRIHSIESFGTLDGPGIRYVLFLQGCPLRCLYCHNPDA
                     GDCEAGRVITAGEALTDILTYRAFIARGGVTLSGGEPLLQPEFVCALLEGCREQCLHT
                     ALDTSGAIPLSISRAALDRADLILLDVKAIDDDLCRTLTGRGNRETLATLEYCEAAGR
                     EVWVRHVLVPGYTLDPAHLTRLADHLLRYACIRKVELLPFHKMGEYKWEALQRPYRLS
                     DTPPPTEEEVQAARAIFEQRGLL"
     gene            complement(4232..5092)
                     /locus_tag="PK576_06025"
     CDS             complement(4232..5092)
                     /locus_tag="PK576_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE61187.1"
                     /translation="MWSFSSAFSLPAIGLVLTLPFATLQAQQPGVTNNIAYYDDDAPQ
                     TDDAYRQSQCRLDLRYPVSKTGFATVVWFHGGGLTSGRRHFIDLKDPSIAVAAVGYRL
                     SPQVEHPGYLEDAAAATAWVLRNIGRFGGDTHKVFVSGHSAGGYLSAMIGMDPRWLAR
                     HGLSHRQLAGIVPISGQVTTHFHVKKLRGDTQSQYRPIIDEFAPLYYSSAELSPICLI
                     VGDRRIEYKNRVEENDLLATSLRNLGHQHVEFYEMGGLNHSTVGQGAMILLREFVGKL
                     SRPSDAAKTK"
     gene            complement(5180..6337)
                     /locus_tag="PK576_06030"
     CDS             complement(5180..6337)
                     /locus_tag="PK576_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61188.1"
                     /translation="MRILKMGQVGGGIGAFIGEVHRKAARMDGGIELTAGAFDVVPSK
                     SLRQGKNLGLDPKRVYSTYEEMIAKESALPPGERVDFVSVCTPNHTHFPIAKACLKAG
                     FHVMCEKPMTLTVAEAEELAALVKSSGKVFGLMHNYTAYPMVKLARDMVKQGDLGTLR
                     KVVVQYPQGWLYQLAEKSGNMQASWRTDPKTSGVAGAMGDIGTHAANLAEYITGVKIT
                     RVLADLTAFVKGRKLDDDGNVLLRFQKGVKGVLHASQISVGEENNLAIWVYGDKKGLW
                     WRQENPNELHVLCQTAPEEIWRRGNGYVAEKSKAAARGTRIPSGHPEGFLEAFANNYC
                     NFADTIRAKAEKRKPSPEELDFPSVEAGVRGMKFIHAVVTSSKKGSVWVKI"
     gene            6653..8251
                     /gene="nadB"
                     /locus_tag="PK576_06035"
     CDS             6653..8251
                     /gene="nadB"
                     /locus_tag="PK576_06035"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOE61189.1"
                     /translation="MMRIESCDFFVVGSGIAGLMSALYLAPHGRVLLVTKKRIDDCNT
                     NRAQGGIACVMAAGDSFEQHVSDTLTAGAGLCDEEVVRKIVSTGPARIADLEALGVCF
                     NPRTDGVPGYDLGREGGHSQRRVLHAGDITGQHVEAVLVEQILKHPHIQVLEHTMVID
                     LITTRWLKRPEENRCVGAYVLDQLAGEIYAVRAADTILATGGCGKVYLYTCNPDIATG
                     DGVALAWRAGATIENMEFIQFHPTCLYHPQAKRFLISEAVRGEGAELVDAEGRPFMQK
                     YDPRGALAPRDVVARAIDSEIKRTGAPYCCLDIRHKSRAFLQKRFPTLYKTCASFGID
                     MAHDLIPIVPSAHYCCGGIRASVDGVTSLPGLYAVGETACTGLHGANRLASNSLLEAL
                     VCAYETAGRIAAQPGRLPDAIRIPDWQYGTAVASDEAVMVAHNWMEVRTCMWDYVGIV
                     RTNKRLERARRRLRNLRREIREYYFDYLVTADTLELRNLALVGELIVRSAVRRRESRG
                     LHYTLDYPETKRFARNTSLPGRNA"
     gene            8267..9346
                     /gene="queA"
                     /locus_tag="PK576_06040"
     CDS             8267..9346
                     /gene="queA"
                     /locus_tag="PK576_06040"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE61190.1"
                     /translation="MLTSAFDYDLPPDLIAQEPPAVRGTSRMMVLHGSDGRIEHRHVM
                     DLPAYLGADDLLVLNDTRVFPARLQGFWTDTHGALELLLLEPCPAPEGTACAEGTVCW
                     ECITGSGRPIRPGQRAVFADGQIEAEMLARQPNNAWRIAFRSPVPLMTLLERYGVTPV
                     PPYIHRCGDARQARLDRERYQTIYARETGAVAAPTAGLHFTEALFAALAARGVARAAV
                     TLHVGPGTFKPVKAEQVEEHRMDPERYAVPAATAAAVATCRARGGRVVAVGSTSVRAL
                     ETVAAAHGGEVTAAVGSSSIFIYPPYRFRVIDAMLTNFHLPKSTLIMMVAALAGRERI
                     LEAYREAVRERYRFFSYGDCMLILP"
     gene            9395..10144
                     /locus_tag="PK576_06045"
     CDS             9395..10144
                     /locus_tag="PK576_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOE61191.1"
                     /translation="MSEKTLIIIPTYDERENVEPMSEAVLREVPTANILFVDDHSPDG
                     TGAVLDAIAAREPRVFVMHRAGKEGLGRAYIAGFKWALERGYELICEMDCDFSHDPAA
                     LPVLLQTAREGADLVLGSRYIGGIRVMNWPMSRLLLSTGAAQYVKLITGMPVNDPTGG
                     FKCFRRAVLEAIDLDRITSNGYSFQIEMTHTAWMLGFTVREVPITFEDRRSGYSKMRA
                     NIAYEAVAMVLRLWGRCGFRRSPRPRPREET"
     gene            10141..11058
                     /locus_tag="PK576_06050"
     CDS             10141..11058
                     /locus_tag="PK576_06050"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOE61192.1"
                     /translation="MIPTRAAIWFRALRPAQWLKNGVLPAAYFFARWDPTQCEGVRGV
                     LPLVYVALAVACFCCVSSGIYLINDVRDIEADRAHPLKRLRPLAAGQIRASAAVAVAI
                     VLLAAGLAAGALTLPRAFTEVLLAYGLMQLAYTFGLKRIPYVDVFVIAVGFVLRAIAG
                     AAALSVRISPWLLLCTFLLALFLALCKRRHEKVLLDEDGERHRKALAGYDRYLLDLQI
                     AVTASATLVCYAMYTLSEETVRRFGTNRLGFTIPFVVFGLFRYLELVYRHDGGGRPEQ
                     TLLTDRVLIGTMILFLLTALAVFLTGQTV"
     gene            11055..11855
                     /locus_tag="PK576_06055"
     CDS             11055..11855
                     /locus_tag="PK576_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE61193.1"
                     /translation="MTRAFLKTLIRRYRAYVDSFRDARGGLSELHRLKIEHTERVAEN
                     ACRIMSGENWPEAWTAAGEACAWLHDIGRHVQLRDYGTFRDADSLDHAACGVAVIREE
                     RLLDGLAAASRARILAAVALHNRKTVPDGLADDAARLTHLVRDADKLDIFRVLEEVVD
                     DGSLRRNPAIAWGLRLDGAPSPDVVAAVCAGQPVSYDAVRNLSDFVLIQVGWLINGLH
                     YTASLRLAAERRVLEYREAFLKTLSTDAGVTRCCDAARRHFAAKAVGA"
     gene            11852..12733
                     /locus_tag="PK576_06060"
     CDS             11852..12733
                     /locus_tag="PK576_06060"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOE61194.1"
                     /translation="MRACVLVAPTVGAGPRERLNAALARVSDAHGMAVEVLDLPAADH
                     LADEIRAILDRGVECVVAAGGDGTVAAVAQVLVGGGVPLGIVPMGTGNLVARELGIPL
                     EVERAAERLACPSSFRRLDVMRINGRIYLLNAGVGVNAEVIDRTSRRGKTLFGVSAYV
                     GTAVWKVLQARPHRLEIVIDGVSMVYDATDVLISNCGILARGLYPNGPDIRADDGRLD
                     VCVVCMKRPIEYPWSYVMRLLRMRNVNRVLHEHAAACAVTVRSARPLVVQADGDIIGS
                     TPVTVEILPHALSVIVP"
     gene            complement(12767..14059)
                     /gene="murA"
                     /locus_tag="PK576_06065"
     CDS             complement(12767..14059)
                     /gene="murA"
                     /locus_tag="PK576_06065"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOE61195.1"
                     /translation="MSRFIIKGGRPVSGVHPVPGNKNAALPMLAASLLTAEPVVLSNL
                     PLIADVRTMIDLLVELGAQVELDRARRTVRICAQRIKDTHLNKTLCGKVRSSILFAGP
                     LLARCGSAKLYPPGGDVIGRRRVDTHLDGFRQLGASVRAGRTYLFKAAKRLEGQRILL
                     DEASVTATENLIMAAALARGTTLLYNAACEPHVQDLCRMINAMGGRISGIGTNLLTIE
                     GVESLHGTKQRVGPDYIDAGSYMVAALVTGGELTVEGVEPSDFEALERPFKRLGAKWS
                     FDREARSLRLPARQRLKTAYDLGDAIPKIEDGPWPMFPSDLMSVLIVLATQTRGTTLF
                     FEKMFESRMYFVDHLIGMGARIVQCDPHRVVVTGPTILHGTTVASPDIRAGMALLVAA
                     LCAKRETVIYNAGSIDRGYESVEVELSRLGAEIERVPT"
     gene            14312..>14597
                     /locus_tag="PK576_06070"
     CDS             14312..>14597
                     /locus_tag="PK576_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOE61196.1"
                     /translation="MKKTSLILALDQGTTSSRAILFDRELKIVATAQQEFGQFYPQPG
                     WVEHDPEEIWRSQRKVMADVVKQTPDGPAAVAAIGVTNQRETVVVWNRRTG"
BASE COUNT         2618 a         4408 c         4697 g         2874 t
ORIGIN      
        1 atcccgatag cgatcccgat cccgatttgg atgaaaccct gaaaccctga aaccctgaaa
       61 ccctgaaacc aaagagagtt gaaggttcaa ctactctttt taggttaacc tgacacgcta
      121 cgggtcaagg cggacgtttt ccatcggcgc ggaaatctca tatttcttgc cgcccggcga
      181 ttcggtgaca gaaacgaccg aggagtcgct cagcggcccc ctgatccaga ctgcgacctt
      241 ccccgaagct tttacgcccc ggacatccac atggtgcatt tctctcaact cgcaccgtct
      301 tgtcctgtgg taactcttgt ctccgatgcg gattgtcgct tgaggacgcc tctcgttccc
      361 cgttgccgta tcgacgacat cctcaaccgt gatgtggtgc atccggatgc catcacacgt
      421 cagcagacgg atgacgccat gccctcccga cgaacgggcc cggatattgc gaatcgtcac
      481 gttgtagatg tcatcttttt cgcccagcgt cgcattgccg ccgatctgca tgccgaagcg
      541 cgctggagag ccggggggat cacgataagc cgtcagcgcg accacatcgt cacctgtcat
      601 gccggagatg ttctcgatca caaggtcgtg gcatcccttg cgcacgtcaa caccgtcctg
      661 attgaacagg atgtccgtcg agccgaagac gatatcgtgt atgtgaccgc ggcaacagcg
      721 ttcttgggaa atcgcccagc actgtgtctc ctccatcgtg aacccgccga cctcgtagtc
      781 gctcacgtca caaaggagaa tgccgaccga ccgccaaccg ttcggatcgc ccgagcgccg
      841 gggcagatag tggttgcgtt ttccgccgct caaaaccgct ccattcttgc caagaatacg
      901 aatgccgcgg ttcggcgtac accgcccctc tacggcaccc gcgttgcgca caagattgtc
      961 ctgtgtcccg tccacaagct ccacgcggca accatcaaag acaagcgtga agtcatctgg
     1021 cacgaggatt gcccgcgtca ggtgccaagc gccgtccacg gatgccgcat tcttcgtcaa
     1081 cgtcacggcg cgcgagccgt cgacgcatgc cgccgcgatg cgacgctcga tcgcgtccga
     1141 gtctgccgtg gacggggcct tgagctgacg gtccgcgaaa tccatgaacc gctcccagtc
     1201 gtcggcacga agtttgtgag gcccgtcgtg gcagtggtaa cccatgcgtt cttcaagttt
     1261 atacgcgcga tagacgtcgt gggccgcatc ccaggccgcc ttctccgccg ccggccccgc
     1321 ccaggcatcc tcgctcccgc tcgccacata gacgagtcgc ggggcgatca ggcggatcag
     1381 gtcatccgcg tcatgcggta actcggcatc ccggtcgatc cacgcagtga aggtgggaca
     1441 aaaccagttc gggaagttct tgaggatctg ccggatggtc tctgccttcg gagccttcgc
     1501 tcgtccgagc cgtgcgccgc cgcaaccgga accgttagag atcgtcatcg cgaagcgttc
     1561 gtcctgtgcg cccgtccaga gcgcggtctt cccgccgcgg ctatgcccga gcacagcgac
     1621 acgcgacgcg tcgagttcgg gccgggtctc gatccagtcc atcacccgcg agttgcccca
     1681 ggcccacgcg ccaatcgttc cccaagagtt cgcctggcga acgacgttcg tcgcattccg
     1741 gtcgcctgtc gcggcatagg cgatgatacc aagcggccac cgtccgaatg aatggtttgc
     1801 atccacattc gggcagacgt cattgaagtt ccaccgcaca taggcgtagc cgcgagccgt
     1861 gatcgagttt gtcgggatcc cctcgtacgt ctcctgcgcg aagtccggcg gcgcattgcc
     1921 gccccgatga tcaccgaaga gaaaaaccgg tgcggggtgt gcggcatcag ctccggcagg
     1981 cagactgcaa tggatttcga tgcggacacc cgcattggtg aacgcgacgt acgtttcgcc
     2041 aatcacctcg tccggcaagc ggccgaccgg cgtcttgccg aactggttct cctcgaacca
     2101 tgccagcgtc tcttgtcgct ttccgagttc ccacgccttc acttgcggtg caatgccgtt
     2161 ccacaggcgc ggcgtggact gcgccccatc gggacatgcc ctcacgcaca ccgcaggtga
     2221 cggaaccgat ccgccccagc cggaagcgac cacacagaat gccatgactc ccaaaacaag
     2281 tgctttcatt tcaaccatta tccggaatag aaaaggccgc gtcaattccc aatcgtgttt
     2341 tgtcgcgatt ccaagcccat tccatttccg agccgttggc cattgcggtt tccagtttcg
     2401 ggttggggta actccacccc ctctgcctgt aacccggtca ttttgtcata tcgcgacagt
     2461 cccggattga caccgggacg gcattccttc acaatatcct cacaatttgt ctggtatagc
     2521 gtttcttgcg ggcgtgaact ggaatccgtt tgaaacgcat gaaaggaacg tatgcaaagc
     2581 ctgctcaatc atttaaacca gcttcaggaa ctcgtgttga tccgggatga gcaccgtacg
     2641 acaggggacg gctcccacat ggagcgtctg aacgactcga tcgatcagtt gacggacaag
     2701 ctgccgccgg aaatcaaatc gctctatcaa cggctgtaca aaaaagacca tatcgtgatg
     2761 gcgccgatgt acaacgggtg ttgcgcgatc tgcgggatgc ggttgccgat cagtcaggtt
     2821 cagtccgtgc gtctctgcaa gcagatccag aactgtccga gctgcgcacg catcctgttc
     2881 gaggagagcg gcgcgccacg ctgggtcgga gacgtgccga gccgtaccga gccgcgcaag
     2941 tcgggcattt cgcgtttttc ggctgaatcg ctgatgattc cggatttgaa ggccacgaac
     3001 ggtcgtgagg cgatcgtgga gctggcgacg gtcatggagc agcagaaatt cgtttccggc
     3061 gcggaaaaac tggtggcgag cgctttggag cgggaggcgg ttctgagtac ggcgatggat
     3121 aacgggctgg cctttccgca cgtgcgcggc gtggaggggg ggggcttgac gcttgcgttg
     3181 ggtgtcgcga aaaagggaat ccggtatgat gaagcgggca gcatcgtgaa tctggtcttt
     3241 ttcatcacca tccccacggc ggtcagcgcg ttttacctgc gcctgatgtc aggtttgacc
     3301 gagaccttcc tgaaagagca aaatcgcgag gcgatgcttg cggcggagac gccggagctg
     3361 ctctggaagg cgctgatcaa ggccacccgc tacaccatca aatagctgcg gttcctgcac
     3421 gggagtaacg gctttcgcgg ctgttacgat gcgcccggca acacgccgct acagcaaccc
     3481 gcgctgctcg aaaatcgcgc gggctgcctg aacctcctcc tccgtcgggg gaggggtgtc
     3541 gctgagccgg taaggccgct gcagcgcctc ccatttatat tcgcccatct tgtggaaggg
     3601 cagcaattcg actttgcgga tgcaggcgta gcgcaacagg tggtcggcca ggcgggtgag
     3661 gtgtgcggga tcgagcgtat accccggaac caacacatgg cgaacccaga cttcccgccc
     3721 tgcggcttcg cagtattcga gggtcgccag cgtttcccgg ttgccgcggc cggtcagggt
     3781 gcggcagagg tcgtcgtcga tcgccttgac atccagcagg atcaggtccg cacggtcgag
     3841 ggccgcgcgc gagatcgaga gcggaattgc gccggacgtg tccagcgcgg tgtgcagaca
     3901 ctgttcgcgg cacccttcca gcaaggcaca gacgaattcg ggctggagga gcggttcgcc
     3961 gccggagagc gtgacaccgc cgcgcgcgat gaaggcgcgg taagtcagga tgtcggtcag
     4021 ggcttcgcca gcggtgatga cccgtcccgc ctcgcaatcc cccgcgtccg ggttgtgaca
     4081 gtacaggcag cggagcggac acccctgcag aaacagcaca tagcggatgc cggggccgtc
     4141 cagtgtgccg aacgactcga tggagtgaat ccgtcccgtg acctgcgggt tcatcgcaaa
     4201 ggtccttttt agagggtgcg tgccgcgcgc gtcatttcgt tttggctgcg tcggaagggc
     4261 gcgagagctt gccgacgaat tcacgcagga ggatcatggc accctgtccc acggtgctgt
     4321 gattgagtcc gcccatttca tagaactcaa catgctggtg tcccaagttc cgcaggctgg
     4381 ttgccaacag gtcgttctcc tccacacggt ttttgtattc gatacggcgg tctccgacaa
     4441 tcaggcagat cggcgaaagc tcggcggaac tgtaatagag cggcgcgaac tcgtcaataa
     4501 tggggcggta ctgcgactgg gtgtcgcccc ggagtttctt cacatgaaag tgcgtggtga
     4561 cctgtccgct gatcgggacg attcccgcaa gctggcggtg ggagagtcca tgacgggcca
     4621 accaacgggg atccatgccg atcatggcgc tgagatagcc gcccgcggag tgtccggaga
     4681 caaacacctt gtgggtatcg ccgccaaagc gtccgatgtt gcgcaacacc caggccgtgg
     4741 ctgccgcggc atcctcaagg taaccggggt gctcgacttg gggtgacaga cggtatccga
     4801 ccgccgctac agcgatcgac ggatctttga ggtcaatgaa gtggcggcgt ccggacgtga
     4861 ggccgccgcc gtgaaaccag accactgtgg cgaagccggt cttggatacg ggatagcgca
     4921 gatccagacg gcactgcgat tgccggtaag cgtcgtctgt ttgcggcgcg tcgtcatcgt
     4981 aataggcgat gttgttcgtg acgcccggtt gctgcgcttg tagcgtcgcg aacggcaggg
     5041 tcagcaccag accgattgcg ggcagggaaa acgcagaaga aaaggaccac atggaaagac
     5101 tccttcaagc gtggagggta aaaagcgggg gcaggcatcg gtatgcccac cccacgtgtc
     5161 acagatggaa acaggcaggt tagatcttga cccagacact gccttttttc gaggaggtca
     5221 ccaccgcgtg gatgaacttc atgccgcgga cgccggcttc gacgctcggg aagtccaact
     5281 cttcgggtga gggtttccgc ttttcggcct tcgcccggat ggtgtctgcg aagttgcagt
     5341 aattgttggc aaaagcctcc agaaaccctt ccgggtgccc gctcgggatg cgggtgccgc
     5401 gcgcggccgc cttgctcttc tccgcaacgt acccgttgcc acggcgccag atctcctcgg
     5461 gcgcggtctg gcagaggaca tggagttcgt tggggttctc ctgccgccac cagaggcctt
     5521 ttttatcgcc atagacccag atggccagat tgttctcctc gccaaccgag atctggctgg
     5581 catgcaggac acccttgacg cccttttgga agcggagcag cacattgccg tcgtcatcca
     5641 gtttccgtcc tttaacaaag gcggtgaggt ccgcgagcac gcgcgtgatc ttcacgccgg
     5701 tgatgtactc ggccaggttg gcggcgtgcg tgccgatgtc gcccatggcg ccggcgacac
     5761 ccgaggtctt ggggtcggtg cgccacgagg cctgcatgtt gccgctcttt tcggcgagct
     5821 gatacagcca accctggggg tattggacca ccaccttgcg caacgtgccg aggtctccct
     5881 gcttcaccat gtcacgggcc agtttgacca tcgggtaggc cgtgtagttg tgcatcaggc
     5941 cgaagacctt gccgctggac ttgacgagcg ccgccaactc ctcagcctcg gcgaccgtga
     6001 gggtcatggg cttttcacac atgacgtgga aacccgcttt gaggcaggct ttggcgatcg
     6061 ggaaatgagt gtggttcggg gtgcagaccg acacgaaatc gacgcgctcg cccgggggca
     6121 gcgcgctttc cttggcgatc atctcctcgt aggtggaata cacgcgcttg ggatccaggc
     6181 cgaggttctt cccctgccgc agactcttac tcggaacgac gtcaaaggct cccgccgtca
     6241 actcgattcc gccatccatc cgggccgcct tccggtgaac ctcgccgata aacgcgccga
     6301 tcccgccgcc gacctgcccc attttcagaa ttcgcatatc ctgctccttt cagttggata
     6361 atcacccgta aaaataggtt ccggctaaag cataatgtgt tgttccgaca aagggaagcg
     6421 caaaatcgct tcaaagagcg gaacgcgtca gtgccttatg ggcgttcccg caggcaacgg
     6481 tggggcagtc gccctgccaa agagcctaaa cgtcgcgggt cgcaagtcgt cgatgtacac
     6541 gaaaacgagg acgggatgct gatcccgttt tcgctcggaa ggacgtccat ccgtatgcta
     6601 tactcacggc atttcgaacg gatgacctgc cgggttcggc gggcggggga ggatgatgcg
     6661 catcgagtcc tgtgattttt ttgtggtggg gagcggtatc gccggtctca tgagcgccct
     6721 gtatctggca ccgcacggca gggtgctgct ggtgacgaaa aaacggatcg atgactgtaa
     6781 caccaaccgg gcgcagggcg gcatcgcatg cgtgatggcg gcgggggaca gtttcgagca
     6841 gcatgtgtcg gacacgctga ctgccggagc cggactctgc gacgaagagg tggtgcggaa
     6901 aatcgtttcg acggggccgg cgcggatcgc ggatctcgag gcactcggtg tgtgcttcaa
     6961 cccgcgcacg gatggcgtgc cgggctatga tctgggacgc gagggcggcc attcgcagcg
     7021 ccgtgtgctg cacgcggggg acatcaccgg acagcacgtc gaggcggtgc tggtggagca
     7081 gatcctgaag catccccata tccaggtgtt ggaacacacc atggtcatcg atttgatcac
     7141 cacccgctgg ctcaaacgac cggaggagaa ccgctgcgtc ggggcgtatg tgctcgatca
     7201 actcgccggc gagatttatg cggtccgcgc ggccgacacg attctggcga ccggcgggtg
     7261 cggcaaggtg tacctttaca cctgcaaccc cgacatcgcc acgggtgacg gtgtggcgct
     7321 ggcgtggcgg gcgggtgcga cgatcgagaa catggagttc atccagttcc atcccacatg
     7381 cctctaccat ccgcaagcca aacgctttct catcagcgag gccgttcgcg gcgagggtgc
     7441 cgaactggtg gatgccgagg gccgtccttt catgcagaaa tacgacccgc ggggcgcgct
     7501 ggcaccgcgc gatgtggtcg cccgcgcgat cgactccgag attaaacgca ccggagcgcc
     7561 ctactgctgt ctcgacatcc ggcataagag ccgggccttc ctgcagaagc gttttccgac
     7621 cctgtacaaa acctgcgcct cattcggtat cgacatggcg catgacctga ttcccatcgt
     7681 gccttcagct cattactgct gcggcggcat ccgagcttcc gtggatggcg tgacctcatt
     7741 gccgggcctg tacgctgtgg gcgagaccgc ctgcaccggt ctgcacggcg cgaaccggtt
     7801 ggccagcaat tcgctgctgg aggccttggt ctgtgcgtat gagaccgctg gccgcattgc
     7861 ggcgcagccg gggcggctgc cggacgcgat acgcattccg gactggcagt acggtacggc
     7921 ggtcgcgagt gatgaggcgg tgatggtggc ccacaactgg atggaggttc gcacctgcat
     7981 gtgggactat gtcgggatcg tgcgcacgaa caagcggttg gagcgcgccc gtcggcgtct
     8041 gcgtaacctg cggcgcgaaa tccgcgagta ctacttcgat tatctggtca cggccgatac
     8101 gctggaactg cgcaatctgg cgctggtcgg tgagttgatc gtgcgcagtg ctgtccgacg
     8161 cagagagagc cggggcctgc actacacgct ggattatccc gagaccaaac ggttcgcccg
     8221 gaacacgtcc ttgcccggac ggaacgcata gggagcggca ggcgccatgc tgacaagcgc
     8281 gtttgactac gatctgccgc ccgacctgat tgcccaggaa ccacccgccg tgcgcggtac
     8341 gtcgcgcatg atggtgctgc atggttcgga cggccgcatc gagcaccggc acgtcatgga
     8401 tctgcccgca tatctcggtg cggacgacct gctcgtgctc aacgacacac gggttttccc
     8461 ggcgcgactg caaggtttct ggacggacac gcacggcgcg ttggagttgc tgctgctgga
     8521 gccctgcccc gcgccggaag gtacggcctg cgcggagggg acggtctgtt gggagtgcat
     8581 cactggatcg ggacggccca tccggccagg gcagcgggcg gtctttgccg atggccagat
     8641 tgaagccgag atgctggcac gccagccgaa caacgcttgg cggattgcct tccgttctcc
     8701 ggtgccgctg atgacgctgt tggaacgtta cggggtgacg cccgtaccgc cgtacatcca
     8761 tcgctgcggg gacgcgcggc aggcccgtct ggatcgtgaa cggtaccaaa cgatctatgc
     8821 gcgcgaaacc ggcgcggtgg ccgcgccgac tgcgggactg cactttacgg aagccctctt
     8881 cgcggccctc gcggcccggg gtgtggcgcg cgccgcggtc accttgcatg tggggcccgg
     8941 cactttcaag ccggtcaagg cggagcaggt cgaggagcac cgcatggatc ccgagcggta
     9001 tgccgtgcct gctgccacgg ctgcggctgt ggcgacctgc cgtgcgcgcg gcggccgtgt
     9061 ggtggcggtc ggcagcacca gtgtgcgcgc gctggagacc gtggccgctg cccacggcgg
     9121 cgaggtgacg gccgcggtcg gctccagttc gatcttcatt tatccgcctt accggttccg
     9181 cgtcatcgat gcgatgctga ccaattttca cctgccgaaa tcgacgctca tcatgatggt
     9241 cgccgccttg gcgggccgcg aacgcatcct ggaggcctac cgcgaggcgg tccgggaacg
     9301 ctaccggttt ttcagttacg gtgactgcat gctgatcctg ccctgaattt ttgtgtggaa
     9361 gactcgtccc ctgaatcggt atactgtaag ccaaatgagc gaaaaaacac tgatcatcat
     9421 tccgacgtat gacgagcgcg agaacgtgga acccatgtcc gaggcggtcc ttcgtgaagt
     9481 gcccacggca aacatactgt ttgtcgatga ccattcgccc gacgggaccg gcgcggtgct
     9541 ggatgcgatc gccgcgcggg agccgcgggt gttcgtgatg catcgtgcgg gcaaggaggg
     9601 ccttggccgc gcctacatcg ccgggttcaa gtgggcgctg gagcgcggtt acgaactgat
     9661 ctgcgaaatg gattgcgact tctcgcacga tcccgcagcc ctgcccgtct tgctgcaaac
     9721 cgcgcgtgaa ggcgcggacc tggtgctcgg gtcgcgctat atcggcggca ttcgcgtgat
     9781 gaactggccg atgagccggt tgctgctctc gaccggggcg gcacagtacg tcaaattgat
     9841 caccggtatg ccggtcaacg atcccacggg cgggttcaag tgtttccggc gtgccgtgct
     9901 ggaggccatc gatcttgacc gcatcacctc caacggctat tcgttccaga tcgagatgac
     9961 ccataccgcg tggatgttgg gcttcaccgt gcgggaagtg cccatcacgt ttgaggaccg
    10021 tcgttccgga tactccaaga tgcgcgccaa catcgcatat gaggcggtgg ccatggtgct
    10081 gcgcctgtgg gggcgctgcg gcttccgccg gtcgccgcgg ccgcgcccga gggaagagac
    10141 gtgatcccaa cgcgtgcggc catctggttc cgggctctgc gtccggccca atggctgaaa
    10201 aacggtgtgc tgcccgcagc ctactttttc gcgcgatggg atccgactca gtgcgagggg
    10261 gtgcggggag tgcttcctct cgtgtacgtg gctctggcgg tggcttgttt ctgctgtgtc
    10321 tccagcggca tctatctgat caacgacgtg cgggatatcg aggcggatcg ggcgcacccg
    10381 ttgaaacgtc tgcgcccgct tgccgccgga cagatccgcg cgtccgccgc ggtcgcggtc
    10441 gcgatcgtcc tgcttgcggc cggtttggcg gctggcgcgc tgactctgcc gcgcgcgttc
    10501 acggaggtgt tgctcgcgta cggacttatg cagttggcct atacgtttgg actcaagcgc
    10561 atcccgtatg tggacgtgtt cgtgatcgcc gttgggttcg tgctccgcgc gatcgccggg
    10621 gccgcggccc tgtcggtccg catctcgcct tggctgctgc tttgcacctt tctgctcgcg
    10681 ctgtttctgg ccctctgcaa acgtcgtcac gagaaggtgc tgctggatga ggacggggag
    10741 cgccaccgta aggccctggc cgggtatgac cggtatctgc tcgatctgca gatcgcggtg
    10801 acggcttccg ccacgctggt ctgttacgcg atgtacacgc tttcggaaga gacggtgcgg
    10861 cgcttcggca ccaaccggtt agggttcacc atcccgtttg tggtgttcgg tctgttccgc
    10921 tacctcgaac tggtctaccg tcatgacggt ggcggccgtc ccgagcaaac gttgttgacc
    10981 gaccgcgttc tgatcggaac gatgatcctg tttctgctga ccgcattagc ggttttcctc
    11041 accgggcaga ccgtatgacg cgcgcgtttc tgaaaaccct gatccggcgg taccgggcgt
    11101 atgtggacag tttccgcgat gcgcgcggcg ggttgtccga actgcaccgg ttgaagatcg
    11161 aacacaccga gcgggtggcg gagaacgctt gccggatcat gtccggagag aactggcccg
    11221 aggcctggac cgccgcaggc gaggcgtgcg catggctgca tgacatcggc cggcacgtgc
    11281 agttgcggga ttatggcacc ttccgggatg cggactccct tgatcatgcg gcgtgcgggg
    11341 tggcggtcat tcgcgaggaa cgcctgctcg acggtctcgc ggccgcgtcc cgcgcgcgga
    11401 ttctcgctgc ggtggcgttg cacaaccgca agaccgtgcc ggacggactg gccgatgacg
    11461 cggcgcggct gacgcatctg gttcgggatg ccgacaaact cgatattttc cgtgtgctcg
    11521 aagaggtggt tgacgacggt tcactgcggc gcaatcctgc gatcgcctgg ggattacggt
    11581 tggatggcgc accctcgccg gatgtcgttg cagcggtctg cgccggtcag ccggtctcct
    11641 acgacgccgt ccggaacctg tcggattttg tgctcatcca ggtcggctgg ctcatcaacg
    11701 gactgcatta taccgcatcg ttgcggttgg cggcggagcg gcgggtgctg gagtaccgcg
    11761 aggctttttt gaagacactc agcacagatg cgggcgtgac gcgttgttgt gatgcggcgc
    11821 ggcgtcattt tgcggcaaag gcggtggggg cgtgagggcc tgcgtactgg tggcgccgac
    11881 cgttggcgcg gggccgcgtg agcggttgaa cgcggccttg gcgcgggtct ccgatgcgca
    11941 cggaatggcg gtggaggtgc tggacctgcc tgctgccgat catctggcgg atgagatccg
    12001 tgcgattctg gaccgggggg tggagtgtgt ggtggcggcc ggcggggacg gtacggtggc
    12061 agccgtagcg caggtgctcg ttggcggcgg tgtgccgctg ggtattgtgc cgatgggtac
    12121 cggtaatctg gtggcgcggg aattgggtat tccgcttgag gtggaacggg ccgcggaacg
    12181 gttagcctgt ccgtcgtctt ttcggcggct ggacgtgatg cggatcaacg ggcggatcta
    12241 tctgttgaac gcgggcgtgg gggtcaacgc cgaagtgatc gaccgcacct cgcgtcgcgg
    12301 caagactctg tttggggtgt cggcatatgt ggggaccgcg gtgtggaaag tactgcaggc
    12361 ccggccgcac cggttggaaa tcgtgatcga cggggtgtct atggtgtatg atgccacgga
    12421 tgtgttgatc tcaaactgcg ggattctagc gagagggttg tatcccaacg ggccggacat
    12481 ccgtgcggac gacggacggc tggacgtttg cgtggtctgc atgaagaggc cgatcgaata
    12541 cccgtggtcg tatgtgatgc gcctgctgcg gatgcgcaat gtgaaccggg tcctgcatga
    12601 gcacgcggcc gcctgtgcgg tgacggtgcg cagcgcgcgg ccgctggtgg ttcaggcgga
    12661 cggcgatatc atcggctcca cgcccgtgac ggtggagatc ctgccgcacg cgctttccgt
    12721 gatcgtcccg tgagggccgg tggcgcggaa agcccgtacg cggggttcag gtcggaactc
    12781 gttcgatctc cgcgcccaga cggctgagct ccacctccac cgactcatac ccgcggtcga
    12841 tgctgcccgc gttgtagatc acggtctcac gtttggcgca cagcgcggcc accagcagcg
    12901 ccatgcccgc gcggatatcg ggactggcca ccgttgtgcc gtgcaggatc gtcggaccgg
    12961 tcacgaccac ccgatgcgga tcgcactgca caatgcgcgc gcccatgccg atcaggtggt
    13021 ccacgaaata catgcggctc tcgaacatct tttcaaaaaa gagcgtggtg ccgcgcgtct
    13081 gcgtggccag cacaatcaac acactcatca ggtccgaagg gaacatgggc cacgggccgt
    13141 cctcgatttt ggggatcgcg tcaccaaggt cataagcagt cttaagccgc tggcgcgcgg
    13201 gcagccgcag gctgcgtgcc tcgcggtcga agctccactt ggccccgaga cgcttgaacg
    13261 gccgttccag cgcctcgaaa tcggacggct cgacaccctc gaccgtcagt tcgccgccgg
    13321 ttaccaacgc tgccaccatg tagctgcccg catcgatata gtccggcccc acccgctgtt
    13381 tcgtaccgtg taacgactcg accccctcga ttgtcaggag gttcgtgccg atgccgctga
    13441 tgcgaccgcc catcgcattg atcatgcgac agagatcctg cacatgcggc tcgcaggccg
    13501 cgttgtaaag aagtgtcgtc cccctggcca atgccgccgc catgatcagg ttttcggtcg
    13561 cggtcacact cgcctcgtcc agcaggatac gctgtccctc aaggcgcttt gctgctttaa
    13621 agagataggt gcggccagcg cggaccgaag ccccgagctg ccggaagccg tcaagatggg
    13681 tatcgacgcg acgccgtccg atcacatcgc cgcccggcgg atagagcttg gcggagccgc
    13741 agcgcgccag caacggcccg gcgaagagga tcgaggaacg caccttgccg cacaacgttt
    13801 tgttcaagtg cgtgtccttg atacgttgcg cgcaaatacg caccgtccgc cgcgcgcggt
    13861 ccagttcaac ctgcgcaccc agttcgacga gcagatcaat catggtgcgc acatctgcga
    13921 tcaacggcag gttgctgagc accaccggct ctgcggtcag cagacttgcg gccaacatcg
    13981 gcaatgcggc gttcttgttg cccggtaccg gatggacacc gctgaccggt cgaccgcctt
    14041 taatgatgaa tcgtgacatg ggaaatcaac cgttcttggg ggttgttaaa aacaaacgca
    14101 ttgtgacata gaagggcggt gcgcgtccag tttctcatgc ccgtccagtt cggttttgtt
    14161 tgaatgcggt aacgtgttga cttggcatga taccgcatgc taacttcccc acattggaaa
    14221 atgtggacac cggcggggtg ctgacaaggc gccaccgcag tgcgtgcgaa tcaatccgca
    14281 aacgggtgtg agcggaacgt tgaggcctgt catgaaaaaa acgtctctca tcttggcgct
    14341 tgaccaggga acaaccagtt caagggccat actcttcgac cgggaactca agatcgtcgc
    14401 gacggcccag caagagtttg gccaatttta tccgcagccc ggatgggtcg aacacgatcc
    14461 cgaagagatc tggcggtcac agcgcaaagt catggccgat gtcgtgaaac agacgcccga
    14521 cggcccggct gccgtcgcgg cgatcggcgt gaccaaccag cgggagaccg tcgtcgtctg
    14581 gaaccgccgg acgggag
//
LOCUS       DAOSLE010000067        14257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4352_length_14257_cov_9.244543, whole genome shotgun sequence.
ACCESSION   DAOSLE010000067 DAOSLE010000000
VERSION     DAOSLE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4352_length_14257_cov_9.244543"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /locus_tag="PK576_06075"
     CDS             <1..1762
                     /locus_tag="PK576_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOE61197.1"
                     /translation="VLGPMGGVGGEARRVEQQALTPVVPSVTRKSEAAPIPAPQAPAV
                     PGGKDDGPAWVIRAITLKGDLSFAEKRGVKGALEAALAGETARSRQEVQEALRKVFND
                     FIRDGYYLARVSLPRDPYDAKEKTLTVLVEEGLFGQVRIGFADDREDGRWFSREQIAR
                     RFRDIRPGDTFNYKTLYDCLYDANAHPDLTLDTKIAVRKPLEGEGDARRVVRYADIDL
                     TVKESLPFHAVFDINNYAIEAIDEWQASLTLQYLNLTKADDVLTVSPSMSLDSSLMSL
                     AGSYMRPHDIWKGGATTVYAGWSELDCDDVVPFIDLEGTGWFVGLVHSHTLVEDEDRL
                     ISLSGGIVYRYIEDQFSAFNTSLQQRDVSVLPLSLALSYSARRADAFRGRNFATLQGV
                     YNLAAGGGNELEDMWVDAEDDYLIGRLQLARLQPLFGTLDNMQRQVHQWILFFKAEGQ
                     YASCPLIPAEKLALGGFNTVRGYQTKGYLGDNGLYGTVELRTPILLDMVSHTLGRKPG
                     STPLDRLQFVAFTDFGFIEFIDPLPGVDDNETLLSVGAGVRLAVMRYSQLRFDVGVPV
                     ASASGDDDSSEYYLSWQLQF"
     gene            1803..7541
                     /locus_tag="PK576_06080"
     CDS             1803..7541
                     /locus_tag="PK576_06080"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOE61198.1"
                     /translation="MNTRHTILFAGKPVRRVLSAVAAFSVLMTSMPVHALDWANATPV
                     AGGITGGTVGNVTTINQAEQAGIINWTDFDIAAGEIANFSQPDAAAVTLNRITSSMDG
                     TQIAGQINANGGVWVVDPNGVFIQNGARINVGAAFVAATMDISNEDFLAGNMNFDGTV
                     SGTGPVQVDSGASIAAGTLAALLGGEVSMNGVLTTPQAVLAAAGDSIIIDTVNGGTIS
                     ITLAGDAANDMQLGGTFSGDVTAAVEGQGIAQDLVGALEIAGTAELTASGAVTLDNAA
                     NDFQDGVTAHAGLSLKLADTDDIILTDVDTVNGAITVTAGGQITATDVAGLTDNDAND
                     IVLTGEGIAVGVISAGAGTAADVVLDAGTGAITDLQADTVTVGAQGFAQNAGRTVNVV
                     ADQLGMTAQGDIGAAGNPLDLTVKTVAASSANGSVYLYETDMLTVGTVGTVSGVTAGQ
                     NAKVETINGTLTVGQTVTATAGDLLLAANGAGSDVILNAAAGAAADNVTVLASDAFRQ
                     NADITAGGTIDVRTTGNIVMAPDTTSASGGSAGDNIRYFSSNGAVTMGHLNAGDGLVA
                     VDASRGIVSAGGAVNVTASGAQFSSQGGDIGAIPGIGNGPVLTDVDVLAAHSYGSQNF
                     IQVAAGGDVTVGTVGTPDDPETAGVDERAFVNRVGLDSSLTEVYLDAKSDLQSESGDI
                     RLATEWGAITVSDGLADAQSSDMDGVGVRAAGNILLDAWSMNGNVDGDVTLNAAVVSD
                     AGSISVLAARNIAQNVSGSAKGDILVVSGDGTIDVEVAGGAIVMADGTLGQTDGGHIR
                     YAASGGITLGLLDARTREARDVAEDGSDNDADSLVDSADPDEQNWNRDTDWGLVSVWD
                     DTGSVTDGNGGARNIIARGAVFTVPAGGIGEAEGVGGGPVDLQVGTLAALSQGNQYFI
                     QAPDGRDLTIGTVGDGGEQINYVMFSPVGVGSWDIERMSDLESTAGSVKVGVRNGSLT
                     VNDGEPMGPRPEMDGVGVRAAEDVLLDAWSMNGNVDGDITLNAAVVSDAGSISVLAAR
                     NIAQNVSGSAKGDILVVSGDGTIDVEVAGGAIVMADGTLGQTDGGHIRYAASGGITLG
                     LLDARTREARDVAEDGSDNDADSLVDSADPDEQNWNRDTDWGLVSVWDDTGSVTDGNG
                     GARNIIARGAVFTVPAGGIGEAEGVGGGPVDLQVGTLAALSQGNQYFIQAPDGRDLTI
                     GTVGDGGEQINYVMFSPVGVGSWDIERMSDLESTAGSVKVGVRNGSLTVNDGEPMGPR
                     PEMDGVGVRAAEDVLLMTGGGADSDVILNAAVAAGDDVTLLASGNIEQAAAGDVTAAA
                     GDVLVEAGAGIVMADGATAAAGDDLRYEAKGGDAKITGLKSTAADGAVMVKAAGGITD
                     AGDMARDVEASAAQLVAGGSVGQKSGEGNGPLDLAVGTVAAKGGDAGSVYLSEADALL
                     VGTVAAVDVNRVKMDSTSDATADGAALSGAVAGQNVKIENGVGAAGGLTVDQAVTAAS
                     GDVLLSAKGGGLSLNAAVTAGDDVTLLASRGIIQSGGITAGGTIDVETAGGSISLAAV
                     SQTSGGNIRYAASGDVVLDGLLDAGAGDVAVEAGGDITDNMGMIIVNANGFAIPNDVV
                     NIVADTVSFFAGGNIGDTGAGTSGTRPFNPITVRANGVAAQGGNIAVYGKPNLTIASV
                     PAVSVTRLTLDDVDQTATSPALSGINAGTGDVNLMADNSVIDGTGAGTDITAGTLTVS
                     AGHIIGAGGGQNTIDPLEVRLNGGHGPYDIWVNNVAGSPDKVVYAFLNTVGGKSSPVA
                     AATQDSGALIFVNGQYVGGDPESVQLFAAVEAFPAETPELKSRQGVFGDPFFLHDQMD
                     INEPVALGLIDLILTGKATITGAPEIPADAYREIFSGGLSPSTSFWFGTGTDDKQEEA
                     ETEEKGLEKGEV"
     gene            7538..9130
                     /locus_tag="PK576_06085"
     CDS             7538..9130
                     /locus_tag="PK576_06085"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOE61199.1"
                     /translation="MTKKITVAGIAGFVLASMFTIGCVLKCGVRDVPVRTLELPSGPG
                     NPRNSEGAFVTLKDGRILYVYTHFTGGGGDHDSAHLAGRYSSDGGRTWTPQDRVIVPN
                     EGGMNVMSVSLLRLQSGEIALFYLPKNNEQDCRPVMRLSRDEGETWGAPVMCITDEVG
                     YYVLNNDRVIQLKGGRLVVPVCLHWPQGASKADWQGVLMCYLSDDNGKTWRRSKTVQK
                     GYDTAGKRIITQEPGVVELKDGRVMMFIRASGGCQYLAWSSDGGDTWSAPVASEIKSP
                     VSPASIKRLPSTGDLLLVWNDHANIPAGLHGRRVPLSTAISKDDGKTWQNTKVLEGNP
                     KGHYCYIAIHPVQDAVLLGYCAMSCLAHSRVTRVPVPWFYAPSPVEKATPVPPLFGQA
                     AKGPFERLETRLGVWTAEAGHAEVYSYARGTGVRIQGGEDRQVTLTLAQPVACQALKL
                     GVERFTIRAPYALVMEAKVDDGWQKVWEQGEKTPVGNAQAVTGTAGKSVTASQFRFRC
                     TSQLGAIIVDMPDAVFNGFFND"
     gene            complement(9159..10856)
                     /locus_tag="PK576_06090"
     CDS             complement(9159..10856)
                     /locus_tag="PK576_06090"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE61200.1"
                     /translation="MCGQQPFFLFSPGRALSATRFVPETLRGAVLFVPPFYEERKGAL
                     PAFTRTARALAEHQIASLLFDFSGCGDSAGDFGNTDPEWMEEDAETALTWLRNALPGL
                     PVALLGLRTGAVLAARLAARHPESALLVGWSPTDGPTFIRQLLQRRMVNDMTAYGKAR
                     ESRAALEERLRHGDAVDLDGYVMTGAFHAWLQCLLPPALRIPVRLFSGGHDPAASRTF
                     AAQAVHAECSELRFPPFWNTVGQVDLTALTDETVACLSACLSGPTRPAKSFPALADCP
                     DSTAEAACLTLPVSPKVRAMLDLPRGTPRAGALFLHGWSGDRTGPHRLFTRFGRLLAA
                     QGTLCLRPDFAGRGLSGGRPEEASIAGMAEGAQAALAALRGRLPPGTPISVVAICSGC
                     KVAITLAAGNPDLANLLLWSPESMGSLRSGATGWRKTRAALLAYARKLTRPETWRKIL
                     TGRVRTDLVTRALVRHETRSAEEAVREDATLARFRAFRNPLLLVFGGSDPDAPGSRTA
                     YTRYCRTHAIPCRTHTIPHAGHSYYGEAWTQELFEVSLAFLQPDRDLPTRSESDAEN"
     gene            10980..11501
                     /locus_tag="PK576_06095"
     CDS             10980..11501
                     /locus_tag="PK576_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE61201.1"
                     /translation="MPLYEFYCEPCHTLYTFRSPRVDTVTLPVCPDCGQELKREISPF
                     SHSVRGKTCADTEEDPGDLDRMDVAAAKLSERLHALERDDADPREAVRVMRELAREGG
                     IRFNREVQEAMARIESGEDPEQVEETFREVFDTENPFEPDNESPGPATTGWWRRIHPP
                     RRDPVWRDLPERG"
     gene            complement(11558..12412)
                     /locus_tag="PK576_06100"
     CDS             complement(11558..12412)
                     /locus_tag="PK576_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOE61202.1"
                     /translation="MNDTEVRFFFLLFAVFSALFGACVGSFLNVCIHRIPRDESVVTP
                     RSHCPHCNTLIPWYLNIPVASWLALRGRCASCKGPIAFRYTLVELLTAALFLAVFLKW
                     AAPAALGTTAIPHPAAIPVYWLFLAGLVLGTFVDFEHFILPDSVTIGGMVAGPILSAL
                     VPSLHGRTVWWQGLLLSLIGLAVGFGLLYAVAWIGGRIFKKEAMGFGDVKLMGAIGAF
                     LGWQAVLFTVFVSSLLGSVCGLLMIAFGGVKLQSRIPFGPYLSAAALIWVFWGEVLFG
                     AYLRLMRG"
     gene            complement(12465..13838)
                     /locus_tag="PK576_06105"
     CDS             complement(12465..13838)
                     /locus_tag="PK576_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE61203.1"
                     /translation="MKRCLLLASLACVGTLAAQTVYTSDWESIDKRPVPQWWCDAKFG
                     IFIHWGVYAVPAYAPTDVANVYAKYSEHYDNRMRHNNPAFTSFHARVYGPHVTYADFA
                     TRFRAEHFRPEQWAALFKKSGARYVVLTSKHHDGYALWPSDYSPRWNSQVLGPHRDLA
                     GELTDAVKAAGLHMGFYYSLLEWGNPLYTKETIGRWVAEMNLPQMKELVTRYKPDIVW
                     TDGEWDFTDVELRSTDFLAWLYNESPVKETVVVNDRWGKKLRSKHGGHYTTEYSHGSN
                     EANEKQPVVHPWEECRGIGGSFGFNRFETTEHYMTSAQCIETLIEKVSRGGNLLLNIG
                     PQADGLIPVIMEERLLDMGKWLAVNGEAIYGTTTWRHRPKDMGKNHIYFTRKPDALYV
                     LCTRWPQQPIAVSPVSGAAAVTLLGSDAPVAFTCAGDTITLRPPAINPGTAPCDHAWV
                     FKIAHPR"
BASE COUNT         2634 a         4119 c         5025 g         2479 t
ORIGIN      
        1 ggtgctgggg ccaatggggg gtgtgggcgg cgaggcccgc agggttgagc agcaggccct
       61 gacgccggtt gtgccgtccg tgacccgtaa gtcggaggcc gccccgattc ccgcgccgca
      121 ggcgccggcc gtcccgggcg gaaaggacga cggcccggct tgggtgatcc gggcgatcac
      181 gctgaaaggc gacctgtcct ttgccgagaa aagaggcgtg aagggcgcgc tcgaggcggc
      241 gttggcgggc gagaccgcgc gcagccggca ggaggtgcag gaggcgctgc gcaaggtgtt
      301 caacgacttc atccgcgacg gctattatct ggcgcgcgtc tcgctgccgc gcgatccgta
      361 tgacgcgaaa gagaagacgc tgaccgtgct ggtcgaggag ggcctgttcg gtcaggtgcg
      421 gatcgggttc gccgatgacc gcgaggacgg ccgctggttc agccgcgagc agatcgcccg
      481 ccgtttcagg gacatccggc ccggcgacac cttcaactac aagacgctct acgactgcct
      541 gtacgacgcc aatgcgcacc ccgacctgac gctggacacc aagatcgccg tgcgcaagcc
      601 gttggagggc gagggggacg cgcgccgcgt ggtgcgctac gcggacatcg acctgacggt
      661 taaggagagc ctgccgttcc acgcggtctt tgacatcaac aactacgcga tcgaggcgat
      721 cgacgagtgg caggcctcgc tgacactgca gtacctgaac ctgaccaagg cggacgatgt
      781 gctgaccgtc agcccttcca tgtcgctgga ctcctcgctc atgtcgctgg cgggcagcta
      841 catgcggccg cacgacatct ggaagggcgg ggccacgacg gtctacgcgg gctggtccga
      901 gctcgactgc gacgacgtcg tgccgttcat cgacctcgag ggcaccggct ggttcgtggg
      961 tctggtgcac tcccacacgc tggtggagga tgaggaccgg ttgatttcgc tttcgggtgg
     1021 catcgtctac cgctacatcg aggaccagtt ctccgcgttc aacacctcgc tgcagcagcg
     1081 cgacgtctcg gtgctgccgc tgtcgctcgc gctcagttac tccgcgcggc gggccgatgc
     1141 gttccggggc cggaatttcg ccacgctcca gggcgtgtac aatctcgcgg ccggcggcgg
     1201 gaacgagttg gaggacatgt gggtcgatgc ggaagacgac tacctgatcg gccgcctgca
     1261 actggcccgc ctgcagccgc ttttcggcac gctcgacaac atgcagcgcc aggtccacca
     1321 gtggatcctc tttttcaagg ccgagggcca atacgcctcc tgcccgctga tccccgccga
     1381 gaaactcgcc ttgggcgggt tcaacaccgt gcgcggatac cagaccaaag ggtacttggg
     1441 cgacaacggc ctctacggga cggtcgaact ccgcacgccg atcctgctgg atatggtctc
     1501 ccacaccctc ggccgcaagc cgggctccac cccgctcgac cggttgcagt tcgtggcctt
     1561 caccgatttc ggcttcatcg agttcatcga cccgctgccc ggcgtcgatg acaacgaaac
     1621 cctcctgagt gtgggcgcgg gcgtgcgtct cgccgtgatg cggtacagcc agttgcgttt
     1681 cgacgtcggc gtgccggtgg catcggcctc cggagacgac gactcttcgg aatactacct
     1741 gagttggcag cttcagtttt aacggatttc atcaaggact ctctgatcgc aaaggacaaa
     1801 ccatgaatac acgacatacc atcctgttcg caggaaaacc ggtacggcgc gtcttgtccg
     1861 cggttgcggc tttcagtgtc ctgatgacca gtatgccggt gcacgccttg gattgggcga
     1921 acgccacacc ggtcgccgga ggcatcaccg gcggcacggt cgggaatgtg accaccatca
     1981 atcaggccga gcaggcgggc atcatcaact ggaccgattt cgacatagcg gccggcgaga
     2041 tcgcgaattt cagccagccg gatgccgcag cggtgaccct aaaccgtatc acctcgagta
     2101 tggacggcac gcagatcgcg gggcagatca atgccaacgg cggcgtgtgg gtggtggacc
     2161 ccaacggcgt cttcatccag aacggggcca gaatcaatgt cggcgcggcg ttcgtggccg
     2221 cgaccatgga catttccaat gaggattttc tggccggcaa catgaatttt gatggtacgg
     2281 tcagcggcac cggtccggtt caggtggaca gcggtgcgag tatcgccgcc gggaccttgg
     2341 cggctctgct gggcggcgaa gtttcgatga acggcgtgct cacgacaccg caggccgtgt
     2401 tggcggcggc gggtgattcg atcataatcg acacggttaa cggcggcacc atttccatca
     2461 cgctggcagg agacgcggcc aacgacatgc agttgggcgg cacgttcagc ggtgacgtta
     2521 ccgcagcggt ggaaggtcag ggtattgcgc aagatctggt cggtgcgctt gaaatcgcgg
     2581 gtactgccga acttacggcc tcgggcgcgg tcacgcttga taatgcggcg aatgattttc
     2641 aggacggcgt gacggcgcat gcagggctct cgctgaaact ggccgacaca gacgacatca
     2701 tcctgactga tgtggacacg gtcaacggcg cgattaccgt gacggcgggg gggcagatca
     2761 cggcgacgga tgtggcgggc ttgacggaca acgatgcgaa cgacattgtg ctgacgggtg
     2821 aaggcattgc ggttggagtc atctcggcgg gcgcgggcac ggcggcggat gtggtgctgg
     2881 atgcgggtac gggcgcgatc acggatctgc aggcggatac cgtgacggtg ggtgcacagg
     2941 gattcgcgca gaacgctggg cgcacggtca acgttgtggc ggatcagctg gggatgaccg
     3001 cgcaaggcga tatcggtgcc gcagggaatc cgctggatct tacggtcaag acggtggcgg
     3061 cgtcctcggc caacggaagc gtttacctct acgaaaccga catgctgacg gtcggcacgg
     3121 tcggtacggt ttccggggtc acagccgggc aaaacgccaa ggtcgagacg atcaacggca
     3181 ccttgacggt gggacagact gtgacggcca cagcagggga tttgctgctg gcggcgaacg
     3241 gtgcgggctc cgacgtgatc ctgaacgcgg cagccggggc ggcggcggac aacgtgacgg
     3301 ttctggcgag tgacgccttc cggcagaacg cggatatcac ggcgggcggc acgatcgatg
     3361 tgcggaccac cggtaacatt gtgatggcac cggacaccac gagcgcgagc ggcggttcgg
     3421 cgggtgacaa catccgttat ttttcgtcaa acggggctgt cacgatgggg catctgaatg
     3481 cgggtgacgg tttggtggcc gtggacgcat cccgcgggat cgtaagcgca ggcggcgcgg
     3541 tgaatgtgac cgcgtccggg gcgcagttca gttcgcaggg gggggacatc ggtgccatac
     3601 ccggaatcgg caacggaccg gtgttgaccg atgtggatgt gctggccgcg cacagttacg
     3661 ggtcacagaa cttcattcag gttgcggccg gtggcgatgt gacggtcggt acggtgggaa
     3721 ccccggatga tccggaaacg gccggtgtgg atgagcgcgc ctttgtgaac cgcgtcgggc
     3781 tggattcctc gctgactgaa gtttatctcg atgccaagag cgatctgcaa tccgagagtg
     3841 gtgacatccg cctggccacg gagtgggggg cgatcacagt gagcgacgga ctcgcggacg
     3901 cgcagtcctc ggatatggac ggggtcggcg tgcgggcagc ggggaacatc ttgctggacg
     3961 catggagcat gaacggcaat gttgacgggg acgtcacgct taacgcggcg gtggtttcgg
     4021 atgccggctc tatcagtgtg ctggccgcgc ggaacatcgc gcagaacgtc tcgggttcgg
     4081 ccaagggcga catcctcgtc gtgtccggtg acggcacgat cgacgtcgag gtggcaggcg
     4141 gcgccatcgt tatggcggac gggacgctgg gccaaacgga cggcggccat atccggtatg
     4201 cggcctcggg cggaatcacg ttgggcctgt tggacgcccg gacccgggag gcgcgggatg
     4261 tggcggagga cggatcggac aacgacgcgg actcgctggt ggactcggcg gatcccgatg
     4321 agcagaactg gaaccgggac acggattggg gtctggtctc cgtctgggat gacacgggtt
     4381 cggtgacgga cggaaacggc ggggcacgca acatcatcgc gcgcggcgcg gtcttcaccg
     4441 tgccggcggg cggcatcggc gaggcggaag gagtgggcgg cggtccggtc gacctgcagg
     4501 tggggacctt ggccgcgctg agtcagggca accagtattt catccaagcc ccggacggca
     4561 gggatttgac gatcggcacg gttggggatg ggggggaaca gatcaactat gtgatgttca
     4621 gcccggttgg cgtcggatcg tgggacatcg agcgcatgag cgatctggaa tcaacggccg
     4681 gatcggtcaa ggtgggtgtg cgtaacggct ccttgacggt gaatgacggc gagccaatgg
     4741 gcccgcgccc tgaaatggac ggtgtcggcg tgcgggctgc ggaggacgtg ctgctggacg
     4801 catggagcat gaacggcaat gttgacgggg acatcacgct taacgcggcg gtggtttcgg
     4861 atgccggctc tatcagtgtg ctggccgcgc ggaacatcgc gcagaacgtc tcgggttcgg
     4921 ccaagggcga catcctcgtc gtgtccggtg acggcacgat cgacgtcgag gtggcaggcg
     4981 gcgccatcgt tatggcggac gggacgctgg gccaaacgga cggcggccat atccggtatg
     5041 cggcctcggg cggaatcacg ttgggcctgt tggacgcccg gacccgggag gcgcgggatg
     5101 tggcggagga cggatcggac aacgacgcgg actcgctggt ggactcggcg gatcccgatg
     5161 agcagaactg gaaccgggac acggattggg gtctggtctc cgtctgggat gacacgggtt
     5221 cggtgacgga cggaaacggc ggggcacgca acatcatcgc gcgcggcgcg gtcttcaccg
     5281 tgccggcggg cggcatcggc gaggcggaag gagtgggcgg cggtccggtc gacctgcagg
     5341 tggggacctt ggccgcgctg agtcagggca accagtattt catccaagcc ccggacggca
     5401 gggatttgac gatcggcacg gttggggatg ggggggaaca gatcaactat gtgatgttca
     5461 gcccggttgg cgtcggatcg tgggacatcg agcgcatgag cgatctggaa tcaacggccg
     5521 gatcggtcaa ggtgggtgtg cgtaacggct ccttgacggt gaatgacggc gagccaatgg
     5581 gcccgcgccc tgaaatggac ggtgtcggcg tgcgggctgc ggaggacgtg ctgctgatga
     5641 cgggaggtgg cgcggactcc gacgtgatcc tgaacgcggc ggtggcggcg ggcgacgacg
     5701 tgacgctgct ggcctccggg aatatcgaac aggccgctgc cggtgacgtg acggcggcgg
     5761 ctggcgacgt gttggtcgag gcgggcgcgg ggatcgtcat ggcggacggg gcaacggcgg
     5821 cggcgggcga cgacctgcgc tacgaggcga agggcggcga cgcgaagatc acgggcctga
     5881 aaagcacggc ggcggacggc gcggtgatgg tgaaggcggc aggtggcatc acggacgctg
     5941 gcgatatggc aagggacgtc gaggcgtccg ccgcgcagct cgtggcgggc ggctcggtgg
     6001 gccagaagtc cggcgagggc aacggcccgc tcgacctcgc tgtcgggacg gtcgcggcaa
     6061 agggcggcga cgcgggcagc gtctacctca gcgaggcgga cgccctgctt gtcgggacgg
     6121 tggcggcggt cgacgtgaac cgcgtgaaga tggactcgac ctcggacgcg acggcggacg
     6181 gcgcggcgct ctcgggcgcg gtggccgggc agaacgtgaa gatcgagaac ggtgtgggtg
     6241 ccgcgggcgg tctgacggtc gatcaggcgg tcacggccgc gtcgggcgac gtgcttctca
     6301 gcgccaaggg cggcggtctg tcgctcaacg cggcggtgac ggcgggcgac gatgtgacgc
     6361 tgctggcctc acgggggatc atacagagcg gcggcatcac ggccggcggc acgatcgacg
     6421 tggaaacggc cggcggttcg atcagtctgg cggcggtgtc gcagacctca ggcggcaaca
     6481 tccgctatgc ggcgtctggc gacgtggtgt tggacgggtt gctggatgcg ggggcagggg
     6541 atgtcgcggt ggaggccgga ggcgatatca cggacaacat gggcatgatc atagtcaacg
     6601 cgaacggttt cgccatacct aacgatgtcg tgaacattgt ggcggacacg gtgtcgttct
     6661 ttgcaggcgg gaatatcggt gacacgggcg cgggaacttc ggggacgcgc cccttcaatc
     6721 cgattacggt gcgcgcgaac ggtgtggcag cgcaaggagg caacatcgcg gtttacggca
     6781 agccaaatct gacgatcgca tcggtgcctg cggtgtcggt cacccggctg acgcttgatg
     6841 atgttgacca gaccgcgacg agcccggcgc tgagcgggat caatgccgga accggggatg
     6901 tgaatctgat ggcggacaac tctgtcatcg acggcaccgg agccggcacg gatatcacgg
     6961 caggcacact gactgtttca gcggggcaca tcatcggtgc gggcggggga cagaacacga
     7021 ttgatcctct ggaggtccgg ttaaatggcg gacacggacc gtatgacatc tgggtcaaca
     7081 atgtggcggg cagtccggat aaggttgtct atgccttctt gaatacggtc ggcgggaaaa
     7141 gttcgccggt cgcggcggcg acccaagaca gcggtgcgct gatttttgtg aacggtcagt
     7201 atgtcggcgg ggatcctgag tccgtgcagc tcttcgcggc ggtggaggcg tttccggcgg
     7261 agactccgga gttgaaatcg cgccaaggcg tgttcggcga tccgttcttt ctgcatgacc
     7321 agatggacat caacgagccg gtggcgttgg gcctgatcga tctgatcctg accgggaagg
     7381 ccacgatcac gggtgctccg gagattccgg ctgacgctta ccgcgagatc ttctccggcg
     7441 gtctgagtcc gagcacctcc ttctggttcg ggaccggtac ggacgacaag caggaagaag
     7501 cggagactga ggaaaaggga ttggaaaaag gggaagtatg acgaaaaaaa tcaccgtggc
     7561 tggaatcgcc ggtttcgttc tggcgtcaat gtttacgatc ggatgtgtct tgaaatgtgg
     7621 cgtgcgggac gtgcccgttc gcacgttgga attgccttcg ggaccgggta atccgcgtaa
     7681 cagtgagggg gcgttcgtca cgctcaagga cgggcgcatt ctgtatgtgt acacccattt
     7741 cacgggtggc gggggcgatc acgactcggc ccatttagcg gggcgctatt catccgacgg
     7801 cggtcggaca tggacgccgc aagaccgggt gatcgtgccg aacgagggcg gcatgaacgt
     7861 gatgtcggtc agcctgttgc gcctgcaaag cggcgagatc gcgctctttt acctgcccaa
     7921 gaacaacgag caggattgtc gtccggtgat gcggctctcg cgggacgagg gtgagacttg
     7981 gggtgcgccg gtgatgtgta tcacggatga ggtcggctat tacgtgctga acaatgaccg
     8041 cgtcatacag cttaagggcg ggcggttggt ggtgccggtc tgcctgcact ggccgcaggg
     8101 cgcgtccaag gcggattggc agggggtgct catgtgctac ctgtcggacg ataacggcaa
     8161 gacgtggcgt cgcagcaaga ctgtgcaaaa aggctatgac acggctggta agcgcatcat
     8221 cacgcaggag cccggcgtgg tcgagttgaa ggacggccgc gtgatgatgt tcatccgcgc
     8281 gagcggcggc tgccagtatc tcgcttggtc ttctgacggc ggcgacacgt ggagcgcgcc
     8341 ggtcgcttcg gaaatcaaat ctcctgtctc tcccgcctca atcaagcgcc tgccctcgac
     8401 cggtgatttg ctgctggtct ggaatgatca cgcgaacatt cccgcaggct tgcacggacg
     8461 acgcgtgccg ctgagcacgg cgatttcaaa agacgacggc aagacatggc agaacacgaa
     8521 ggtgttggaa ggcaatccga aagggcatta ctgctatatc gcgatccacc cggtgcagga
     8581 tgccgtgctg ctcggttact gcgcgatgtc ctgcctcgcg cattcgcggg tcacgcgcgt
     8641 gccggtgccg tggttctatg cgccgtctcc ggttgagaag gccacacctg ttccgcccct
     8701 gttcgggcag gcggcaaaag ggcctttcga gcggctcgaa acccgtctgg gtgtgtggac
     8761 cgccgaggcc ggtcatgcgg aggtctacag ttacgcgcgg ggcacgggtg tgagaatcca
     8821 gggcggcgag gatcggcagg tgacattgac gctggcgcaa cctgtcgctt gtcaggcgct
     8881 gaagctgggg gtcgagcgct ttactattcg agcgccgtat gcgctggtga tggaggccaa
     8941 ggtcgacgac ggctggcaga aggtctggga gcagggcgag aagacgcccg ttggcaacgc
     9001 gcaggccgtc accggcactg cgggcaagag cgtgacggcg tctcagttcc gtttccgttg
     9061 cacgtcccaa ctcggcgcga ttatcgtcga tatgccggac gccgttttca atgggttttt
     9121 caacgactga ggtgcgcctt ttgatcggcc gcgctctgtc agttctcggc gtcgctttcg
     9181 ctccgcgtcg gaaggtcacg gtccggctgg aggaacgcga ggctgacttc gaagagttcc
     9241 tgtgtccacg cttcgccgta gtagctgtgg cccgcgtggg ggatggtgtg cgtccggcag
     9301 gggatcgcgt gggtccggca gtagcgcgtg taggctgtgc gtgagcccgg cgcgtcgggg
     9361 tccgaaccgc cgaaaaccag cagtagcgga ttacggaacg cgcggaaacg cgcgagtgtc
     9421 gcatcttccc gcaccgcctc ctccgcgctc cgggtctcat gccggactag cgcccgcgtg
     9481 accagatccg tccgcacccg gccggtcaag attttccgcc aggtttcggg tcgggtcagc
     9541 ttgcgcgcgt aagcgagaag cgcggcccgt gttttacgcc agccggtggc gccgctgcgc
     9601 agggatccca tggattcggg tgaccagagc agcagattgg cgagatcggg gttgcctgcc
     9661 gcaagggtga tcgccacctt gcaaccggaa cagatggcta cgacggaaat cggcgtcccc
     9721 ggcggcaggc ggccgcgaag cgcggccaac gcggcttgcg ctccctccgc cattccggcg
     9781 atcgaggcct cctccggccg acctccgctg agtccccgtc ccgcaaaatc cggacgcagg
     9841 cacagggtgc cctgtgccgc cagcagacgt ccgaagcgcg taaaaagccg gtgcggtccg
     9901 gtacggtctc cggaccagcc gtgcaggaaa agcgcgcctg ctctcggcgt gcctcgcggc
     9961 agatccagca tggcgcgtac cttgggcgag acgggaagcg tcaggcatgc ggcttccgca
    10021 gtgctgtccg ggcagtcggc cagcgccggg aacgacttag ccgggcgcgt cgggccggag
    10081 aggcaggcgg agaggcacgc cacggtttca tcggtcagcg cagtcagatc gacctggccg
    10141 accgtgttcc agaatggcgg gaaacgcagt tccgaacatt ccgcgtgtac ggcttgtgcc
    10201 gcgaaggtcc ggcttgccgc aggatcatgg ccaccggaga acagacggac gggaatccgc
    10261 agggctggcg gcaacaggca ctgaagccag gcgtggaacg cgccggtcat gacataaccg
    10321 tcgagatcca ccgcgtcgcc gtggcgcaac cgctcctcca gcgcggcgcg gctctcccgc
    10381 gccttcccgt aagcggtcat gtcgttcacc atgcggcgtt gcaacagttg ccggatgaag
    10441 gtcggtccgt cggtcggcga ccagccgacc agcagcgccg actccggatg gcgcgcggcc
    10501 agccgtgcgg caagcacggc accggtccgc agaccgagca gggccacggg cagaccgggc
    10561 agggcattgc gcagccacgt cagcgcagtc tccgcatctt cttccatcca ttccggatcg
    10621 gtgttcccaa agtcgccggc cgaatcgccg caacctgaaa aatcaaacag cagcgaggcg
    10681 atctggtgtt ccgccagcgc gcgtgcggtg cgtgtgaaag cggggagcgc gcccttgcgc
    10741 tcttcgtaga acggcggcac gaacagcacg gcgccgcgga gggtctccgg cacaaagcgg
    10801 gtcgcgctca aggcccggcc cggggagaaa aggaaaaaag gttgttgtcc gcacatgctc
    10861 acggcacgtt ccccagtgtt aacgcttccc agtatacggg aaaaatcggg cgggacgaat
    10921 aggaaaatgt tggggtttct ggtatcgtaa cccgcacgga ccaaacacgg gaggctgttt
    10981 tgcccttata cgagttttat tgtgaaccct gtcatacgct gtacaccttt cgttcaccgc
    11041 gggtggacac cgtcaccctg cccgtgtgcc cggactgcgg acaggagctg aaacgcgaaa
    11101 tctcgccctt ttcacacagt gtgcggggaa aaacgtgtgc ggacacggag gaggatccgg
    11161 gggatctcga tcgtatggat gtcgcggccg cgaaactgag cgagcggttg cacgcgctgg
    11221 agcgcgacga tgccgatcca cgcgaagccg tgcgggtgat gcgcgaactc gcccgggaag
    11281 gcgggatccg gttcaaccgg gaggttcagg aggccatggc gcggatcgag tccggcgagg
    11341 atccggaaca ggttgaagag accttccggg aggtgttcga cacggagaac ccattcgagc
    11401 cggacaacga atccccgggc ccggccacca ctggatggtg gcgcagaatc catccgccgc
    11461 gccgcgatcc ggtctggcgc gacctgcccg aacgcgggta ggtggaaact tcttgcctga
    11521 tcttgttttc ggcagcgaaa acctttattc ccgtcagtta tccccgcatc agccggaggt
    11581 aggcaccgaa gaggacttct ccccagaaaa cccagatcag ggctgcggcc gagagatagg
    11641 ggccgaacgg gatgcggctt tgcagtttca cgccgccgaa ggcgatcatg agcaggccgc
    11701 agacactgcc gagcagggag gagacaaaaa ccgtgaacag caccgcctgc cagccgagaa
    11761 acgcgccgat cgcgcccatt agcttgacgt cgccaaagcc catcgcctct ttcttgaaaa
    11821 tccggccgcc gatccacgcc actgcataaa gcaacccgaa tcccacggcc agaccgatga
    11881 gcgacaacaa gaggccctgc caccacacgg tgcgtccgtg cagcgacggc accagtgcac
    11941 tgaggatcgg ccccgccacc atcccgccga tcgtgaccga gtcggggagg atgaagtgct
    12001 cgaagtcgac gaaagtgccg agcaccagtc cggcgaggaa cagccagtag accgggatcg
    12061 ccgcaggatg ggggatcgcc gtcgtgccca gcgctgccgg ggccgcccac ttcagaaaga
    12121 ccgcgagaaa gagcgcggcg gtgagaagtt ccaccagcgt gtaccggaat gcgattgggc
    12181 ctttgcatga cgcgcaccgg ccccgcagcg cgagccagct tgccaccggg atgttgagat
    12241 accacgggat cagcgtgttg cagtgcgggc aatgcgaacg cggggtcacc acggattcgt
    12301 cgcgcgggat ccggtggatg cagacattca ggaagctgcc gacgcaggca ccgaacagtg
    12361 cggagaaaac cgcgaacaac agaaagaaaa accggacctc ggtatcattc acaggtacgt
    12421 gacctcaggt tgtgggaacc gtgttttcta cggtcgccga tccgttaccg ggggtgcgcg
    12481 attttgaaga cccaggcgtg atcacagggc gcggtgccgg ggttgatcgc gggtgggcgg
    12541 agggtgatcg tgtcgcccgc gcaggtgaag gcgaccggcg cgtcgctgcc aagcagcgtg
    12601 accgccgcgg caccggacac gggcgagacc gcgatcggtt gctgcggcca gcgcgtgcac
    12661 aggacgtaga gcgcgtccgg cttccgcgtg aagtagatgt ggttcttccc catgtccttg
    12721 gggcggtgcc gccaggtcgt ggtgccgtag atcgcctcgc cgttcacagc cagccatttg
    12781 cccatgtcca gcagccgttc ctccatgatc accgggatca ggccgtccgc ctgcgggccg
    12841 atgttgagca gcaggttccc gccgcggctg accttctcga tcagggtttc aatgcactgc
    12901 gcggaggtca tgtagtgttc ggtggtctcg aaccggttga atccgaatga tccgccgatg
    12961 ccgcggcact cctcccacgg gtggaccacc ggctgtttct cgttggcctc attgctgccg
    13021 tggctgtatt cggtcgtgta gtggccgccg tgtttgctgc ggagcttttt tccccagcgg
    13081 tcattgacca ccacggtttc tttgactggg gattcgttgt aaagccaggc caggaaatca
    13141 gtgctgcgca actcgacatc ggtaaaatcc cattcgccgt ccgtccagac gatgtccggc
    13201 ttgtagcgcg tgaccagttc cttcatttgc ggcaggttca tctcggcgac ccagcggccg
    13261 atggtctcct tggtgtagag cgggttgccc cattccagca aagagtagta gaaccccatg
    13321 tggaggcctg cggccttgac ggcatcggtc agttcgcccg ccaaatcgcg atgcggtccg
    13381 agcacctggc tgttccagcg cgggctgtag tcactcggcc agagcgcata tccgtcatgg
    13441 tgcttcgagg tcagcaccac ataccgcgcc ccggactttt tgaacagagc ggcccactgc
    13501 tccggccgga agtgctcggc acggaaacgc gtggcgaaat cggcgtacgt gacgtgcggg
    13561 ccgtaaacac gcgcgtggaa agaggtgaat gccggattgt tgtgccgcat ccggttgtcg
    13621 tagtgttctg agtatttggc gtatacgttg gcgacgtcgg tgggcgcata cgccgggacc
    13681 gcgtacacgc cccagtggat gaagatgccg aatttggcat cgcaccacca ttgcggcacg
    13741 gggcgcttgt cgatcgactc ccagtccgag gtgtagacgg tttgtgcggc gagcgtgccg
    13801 acgcaggcga gactggcgag aagcaggcat cttttcatgg gacctccgtt acatgacaat
    13861 cacacatttc ggaatatagc ttacaggcaa agggggccgc ggcacaagac ccaatacgcc
    13921 cgcaacccaa ttcaatctaa aaggagcagt tgaacgggtg gaattctagc agggctccgt
    13981 aaacattggg ttttgcggtg cgcccgacgc tctttttgaa ctcttagtcc cagcccttcg
    14041 tcgcgggtcg aatgtcgcgg gtcgcgggtc gacgatcccg atcccgatcc cgatagcgat
    14101 cccgatcccg atcccgatag cgatcccgat cccgaccccg atttagatga aaccctgaaa
    14161 ccctgaaacc agaaaccaga aaccctgaaa ccccgaaacc ctgaaacctt gaaaccaaag
    14221 agagttgaag gttcaactgc tctttttagg ataaatt
//
LOCUS       DAOSLE010000068        14230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4364_length_14230_cov_7.734250, whole genome shotgun sequence.
ACCESSION   DAOSLE010000068 DAOSLE010000000
VERSION     DAOSLE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14230
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_14230_cov_7.734250"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..652
                     /locus_tag="PK576_06110"
     CDS             173..652
                     /locus_tag="PK576_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61204.1"
                     /translation="MAFVGGQSGGGASGPGLLVGRFDHALDPKRRLTVPAGWRDLMGS
                     PAYVYVFPDPNETCLNLIPPAEMEGRLEKLRQRALFDKSASSALRVFGENAEQAVLDV
                     QGRIRIRDRLLAFAGLKDTVVMIGAMNRIQLWSPALRPDTETVDQAALAQACETLAF"
     gene            798..1754
                     /gene="rsmH"
                     /locus_tag="PK576_06115"
     CDS             798..1754
                     /gene="rsmH"
                     /locus_tag="PK576_06115"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE61205.1"
                     /translation="MHLSVLPREVLETLEVIPGGSYIDGTLGAAGHACAILKRAGPGG
                     RLLGIDRDPEALERSAARLSALSGETVLAHGTHGEVRRLAEANGFAAVNGMLLDLGVS
                     SEQLDTPGRGFSFRLDGPLDMRMDPTRGESAAEMLARLDVQSLADVLRRLGEEPQARR
                     VARAICRAQAEAPIVTTGRLAEVVGKALGGRKGPRHPATKTFQALRMAVNREVEELAQ
                     ALEDGLQLLAPGGRLVVITFESVTDRLVKQRFAAHIGRWVSLQQGGERWEGELPPARA
                     VTRRAVTPGAEEIAANPRARSAKLRAIERCERPERVRGKGFC"
     gene            1769..2164
                     /locus_tag="PK576_06120"
     CDS             1769..2164
                     /locus_tag="PK576_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61206.1"
                     /translation="MRKNRKRHARAKVLPPSVVGFFVLLVSMILCYWFMDSKCAQLGQ
                     EIRKHEQKFSALENERVREEARWNAKKTPEKLERAMLQHGLDMNYPTATQVVRMDAAT
                     GAPVPGQLSVIAFRRSRNAGERVAGTVRK"
     gene            2196..3914
                     /locus_tag="PK576_06125"
     CDS             2196..3914
                     /locus_tag="PK576_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE61207.1"
                     /translation="MHDRGIQMRILLVTAALFFALLGLASRLAYLHLANHSKLTREYR
                     RTLLGLRGRIFDCNGDRFPMAVSLPARQFFLDPKSVKPQHDKKQIAETVAACLGLEAD
                     EVYAQFARTDSRYIKLGVSLNDKVFDLLADPETISGIGSEDLVCRSYPQGRRMSHVLG
                     FVNAMGVGGAGIEQQYNRYLTGTPGLIEGEKDAGRREIWSRRTVHVPAIAGSDLYLTL
                     DHNIQYEVELALREVVEKFEAAGGWVIVQRVRTGEILAMASFPDFAPEQYTEESVDVW
                     RNSALAIVYEPGSIMKAITVAAALNERIVTPETVIDAGEGCWMYAGKPLRDHVHGRVT
                     VSTALKKSSNIACAKIGLMLGNKRLETYLRAFNFGEKLGIELPGEERGILARARDWDK
                     LKPTRIPIGQGVAVTGLQMISAYSTLANDGVMMKPYVVRRIVSPSGETIFEAKPEVLG
                     RPVRPEVARAVREMLRGVTEEGGTGKRASVPGYSIGGKTGTAQKAVPGGYSSTDYYAS
                     FVGFVPAGDPVFSVLVSVERPRPQHTGGFVSAPAFAKIAAATARYLEVPPDQPAEETE
                     QVANHP"
     gene            3950..5440
                     /locus_tag="PK576_06130"
     CDS             3950..5440
                     /locus_tag="PK576_06130"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOE61208.1"
                     /translation="MKISEIPELAPLLVSEAPTVAFTGIQCDSRRIRPGDLFAAITGT
                     RDAGGRYAGAALAKGAVAILSETPLPEYHRQVILVRDVRLALACIASALKAWPSRNMR
                     VYGITGTNGKTTTAWLLREMLVAGGAVPGLLTTVQVEYAGRAIPATRTTPDACELQAI
                     LAAMRTSGCDTAVMEVSSHALDQQRVAWTRFAGAAFTNLSRDHLDYHGTMEAYFVAKC
                     KLFDQLAAETPGALAVCVADAGYGDRMTAYLATLPLRRLTCGFTQAADLRAEALAVTP
                     DASTFTLAAPDGRRLALRVCLAGRYNVANMLCAAGLALEAGVPLEKVGETLEAARPCW
                     GRLERVPVDLPATVFVDYAHTDDALTNVLETLREVTRGRLRVVFGCGGERDRAKRPLM
                     GRACAALADDLVITSDNPRSEDPRAIIEEILQGIPEGTSYEVEADRRLAIRRALSQAR
                     EGDVVLIAGKGHESFQELAGRTLPFDDRQVVAEEARILSEARQQDR"
     gene            5467..6861
                     /gene="murF"
                     /locus_tag="PK576_06135"
     CDS             5467..6861
                     /gene="murF"
                     /locus_tag="PK576_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOE61209.1"
                     /translation="MSNKQPQRLDAAAFAAWTGGDAQALPSTFCGVTQDSRRITPGCL
                     YVALRGERFDGHDFVAQALRDGAAAALVETAWSAPSGSSGWPLIRVKDTRQALADAAR
                     AWRRLNRAFILGITGSSGKTTTKEMAAALFAAGGRVCATRGNLNNEVGLPLSLLAMPP
                     ETEYGIFELGSNHPGEIGRLSDTLQPCAAVITSIGCAHIEHFGTQEAIVREKGALLRA
                     LPHGGFAVLNREITGFAQLAEQSGAPVVTVTLTGGGADFSAEATDLQTGALRITEQAT
                     GEETRLCSGLPGAHNASNLLLAFAAARRAGLPACALAQALSGLTLPKMRWEVMVRDGV
                     TWVNDAYNANPQSMAAALKTFAELPCRGRRIVVLGDMLELGEHTEALHREVGRCVAQT
                     APDRLLTVGHDAKLYLAEEAVRAGFPQTKVVCADDARAAGIVLCEWIRSGDSVLLKAS
                     RGMRLEGIVGHEQV"
     gene            6848..8191
                     /gene="murD"
                     /locus_tag="PK576_06140"
     CDS             6848..8191
                     /gene="murD"
                     /locus_tag="PK576_06140"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOE61210.1"
                     /translation="MSKYDGVQALVLGAGISGEAAARLLLARGGRVRVIDEGKADASA
                     AVAQRITAAGGQVTFGAAGLPSEPFDVCVVSPAFALNHPWITACRARGLPMVPELELG
                     SEYWSGRVIAITGSKGKSSLVKWCSDTLTGAGIPASPAGNYGIPLCQLALDRPDLLWA
                     VTEVSSFQMEHTRTFHPEIAILLNLQADHQDRHVDMTEYKSAKMRLFASMCPGDVVLL
                     PAGFEDDGCVPPGVTLWRFGTDVGCDWRYADHAVTGQAEGTRVRVETGGSWFDNEVFG
                     LSLSAGVAALHAAGLDAEQIAAGVRAFEPLPHRMQLVWESRHGVRYVDDSKATSLAAT
                     AAALRMARKPVRLIAGGLLKEPDLEGIKELLTKTTKKGYLIGHCSRQMFQAWSDVIPC
                     EECGTLDQAVAAAAREARAGDTVLLSPGTASFDQFSSYRERGERFTAQVRAVADL"
     gene            8235..9227
                     /locus_tag="PK576_06145"
     CDS             8235..9227
                     /locus_tag="PK576_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOE61211.1"
                     /translation="MNRQVMRVVAGVNLIVGCFLMQGCGMFNRKSDTTSTTLPDGELP
                     PVEVQPLDTTPPPVQPVETSLVELPSVQTSTPYTIQKGDTLSAIAYKYNVRWQDIVAI
                     NPGIVPNRLRIGQVIQLPGQVDLSRARSVPVATPSVRAPKTVAPKAAAAVPGPTITYV
                     VKPGDSLSVIAHKHGIKTAALREANGLKSDKIIVGQKLKVPGAVKTPSAAPVTKKSAA
                     SVQAPKTVAPKPAAAVKTEPAPAVTTTPPPAAEKTTAPAAVESAPVKIETAPPPAAPA
                     AESSTQTYTVKEGEDLYAVAIRWGVSPSDLKALNNLTSSELKAGTVLKIPNAGQ"
     gene            9247..10440
                     /locus_tag="PK576_06150"
     CDS             9247..10440
                     /locus_tag="PK576_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOE61212.1"
                     /translation="MRSTLTVLSLTVVALLGLGIVVLATASAVRAQGLYADPHFFVRR
                     QLIWLGLAAVTVFLINHFDYHWWRNTPLLTVGFYLLVVLALLLVFVPGIGGKVKGSYR
                     WLNLGPFRMQPSEFAKILTVIGISVWMDRIGWRVRQFTQGALLPGCGLALLVVLLVME
                     PDFGSTLVVCVVGGALMYVAGTRFVYLLLFGGLGLAGVGAFLVTNANRMRRIAAWLPE
                     QFRLIIERWLPPGHTEEGASAAKYQLEQAILAFKNGGPSGVGFNQSIQKYNYLPEAHT
                     DFIFAIGGEELGLAFSLFVVAAYTVILFCGIRIALRAADRLGRMLAFGMTLLLVFQAA
                     FNIGVVTGCLPTKGLALPFMSYGGTNLITALFAVGTLLNVGRHVDVFDERMHTQVVRN
                     AAQEV"
     gene            10514..12199
                     /locus_tag="PK576_06155"
     CDS             10514..12199
                     /locus_tag="PK576_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61213.1"
                     /translation="MARGILLAALWAAAGGCRALREPESGPLPIRCYGEEAARLRGLP
                     LTREIPVTSEARDVWMAVLEGELDKPENRRFLEETERLARHFRVLPADVSLRALYRAL
                     MREQVAAYYDPEKKRIAVVEVPGSGYPEGAANRALVDRFVYTHEYCHAVEDVHFDLER
                     LTRAALSDLDANLALTSLVEGNAVLVGADNLCAAWPVNTATPLGAFLVSLVSRLELTD
                     TVGAGLDAYPAFLAGALVRPYLDGAVFSNRLRREAGWQALDRVYRERLPVTTAEILYP
                     ERRYLRPFQPAEISLASDVMGQGPDVMQPVSTNRLGVLGTALWLGGERLASVRDFGFL
                     QGWLGDRVFLAEAPDGTTCTIWLSYWERPGHARAFRKQVLRRLESDAFTGVLHAVRRE
                     GRLVAAVWMSGPGAEEAVCGEKASRALASRVEAETPSWFASWWEDLPSPVSVGPSGCG
                     VLGGWAAELLGGTGFFHVRLACGGLLRVEHTPDRHYWGTAFGLLRHVGDRRSDFTFWQ
                     IPLVASWHAQGQGERRRFRWSVGAGLVATGEPDRIRVLRIPVWRSATAGKNIP"
     gene            12314..13600
                     /locus_tag="PK576_06160"
     CDS             12314..13600
                     /locus_tag="PK576_06160"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOE61214.1"
                     /translation="MESSSYNPFQVAQSQFDKVAAVLELDGGVRDLLRQPMREYQFSI
                     PVRMDDGTVRVFKGFRVQHNDARGPAKGGIRFHPMETVDTVRALSMWMTWKCSVVDIP
                     LGGGKGGVVCDPHHLSLREQEQICRGWIRQVVRNLGPVSDVPAPDVMTNAQHMLWMLD
                     EFETIRGGRYPGFITGKPVGMGGSLGRTEATGFGVVFTLREALRELGIELRKTTASVQ
                     GFGNVAQHAVQLYTELGGTVVAISCWDQTDKTSYTFRKADGVCLKELRGISDKFGGID
                     KNRARELGYEMLPGGAWLEQEVDILIPAALENQVNRDTVQKISKSVRVIAEGANGPTT
                     PEADEVLQQRGIFVLPDFLTNAGGVTCSYFEQVQCNTNYFWEKDEVLAKLDTKMTSAF
                     AAVSDLARRKKLYLRDAAYMIAINRVAEAVRLRGWA"
     gene            13872..>14230
                     /locus_tag="PK576_06165"
     rRNA            13872..>14230
                     /locus_tag="PK576_06165"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2492 a         3876 c         5130 g         2732 t
ORIGIN      
        1 tagcgatccc gaccccgatt tggatgaaac ccagaaaccc tgaaaccctg aaaccttgaa
       61 accagaaacc ctgaaaccct tttaaggcta tttaccccga atattttttc agggatttcg
      121 ggttgacttc tggtggggga aagtgttgaa tgatgtcaca aaaggtcaag gtatggcgtt
      181 tgttggcgga cagagtgggg gcggagcttc cgggccgggt ttgctggtcg ggcggtttga
      241 ccatgctttg gacccgaagc gccgtctgac cgtaccggcg ggctggaggg atctgatggg
      301 gtcgccggcc tacgtttatg tttttccgga tccgaacgag acgtgcctga acctgatccc
      361 gcctgcggag atggaggggc ggttggagaa actgcggcag cgggcgttgt tcgacaagag
      421 cgcgagttcg gcgttgcggg ttttcggcga gaatgcggaa caggcggtac tggacgtgca
      481 ggggcggatc cgcattcggg atcggctgct ggcgtttgcg gggttgaagg atacggtagt
      541 gatgatcggt gccatgaacc ggattcaact ctggtcgcct gcgttgcggc cggataccga
      601 gacggttgat caggcggcgt tggcgcaggc ctgtgaaacc ctggcttttt agaaagttcg
      661 ccgcgtggca gcgaggcgcg gcctgacaag ggatttggat cgcgggcggg atacggggtc
      721 cggaagacgg acgcggattc gggagcgtcg tgtgttgccg cgcgatcatt attgacaagg
      781 atgtgtctgg agggactatg cacctgtcgg tgttgccgcg cgaagtcctg gaaaccctgg
      841 aggtcattcc cggcggcagc tacatcgacg gaacgctcgg tgcggcgggt cacgcatgcg
      901 cgatactgaa gcgtgcgggg ccgggcggcc gactgctggg gatcgaccgg gaccctgagg
      961 ctttggaacg atctgcggcg cggctgtcgg cgttgtccgg cgaaacggtg ctggcgcacg
     1021 ggacgcatgg tgaggtccgg cgcctggcag aggcgaatgg gtttgcggca gtgaacggca
     1081 tgctgctgga tctgggcgtg tcatccgaac agctcgacac gcccggacgg gggttcagct
     1141 tccggttgga tgggcccctg gatatgcgca tggatccgac tcggggggag tcggcggccg
     1201 aaatgctcgc aaggctggat gtgcagtctc tcgcagacgt gttgcggcgg ctcggtgagg
     1261 agccgcaggc gcggcgggtc gcgcgagcaa tctgccgcgc gcaagcggag gcgccgatcg
     1321 tgacgaccgg tcggctggct gaggtggtcg gcaaggcgct gggcgggcgt aaggggccgc
     1381 ggcatcccgc gaccaagacg tttcaggcct tgcggatggc cgtgaaccgc gaggtggagg
     1441 agttggcgca ggcattggaa gacggcttgc agttgctcgc gccgggcggg cggctggtgg
     1501 tgatcacgtt tgagagcgtg accgaccgtc tggtgaagca gcgtttcgcc gcgcacatcg
     1561 gccggtgggt ttcgctgcag cagggcggtg agcgctggga aggcgaactg ccgcccgcac
     1621 gggccgtgac gcggcgtgcg gtcacgcccg gcgcggaaga gatcgcggcg aatccgcgcg
     1681 cccggtcggc caaactgcgg gcgattgagc gctgcgaacg gccggaacgg gtgcgaggca
     1741 aaggtttttg ttgaggaggg attggaagat gagaaaaaat cggaaacggc atgcgcgggc
     1801 caaagtgctg ccaccgtcgg tggtagggtt tttcgtgctg ctggtcagca tgattctttg
     1861 ttactggttc atggactcca agtgcgccca gctcggtcag gagatccgaa aacacgagca
     1921 gaagttttcc gccttggaga atgaaagggt tcgcgaagaa gcgcgttgga atgccaagaa
     1981 gacgccggaa aaactggagc gggccatgct gcagcatggc ctcgacatga attacccgac
     2041 tgccacgcaa gtggtgcgca tggatgcggc gaccggtgcg ccggttcccg ggcaactctc
     2101 ggttatcgcg ttccgccgaa gccgaaatgc gggcgagcgc gtggcgggaa cagtccggaa
     2161 gtaggcggac ttgattccgg gacggagggt tggtcatgca tgaccgcggc attcagatgc
     2221 gtattctgct ggtgaccgcc gcgttgtttt ttgcgctttt gggactggct tcacggctgg
     2281 cctatctcca tctcgcgaac cattccaaac tgacgcgcga gtaccggcgg acactgctgg
     2341 ggttacgcgg ccgtatcttt gactgcaacg gcgaccgctt tcccatggcg gtcagtctgc
     2401 cggcgcgcca gttctttttg gatccgaagt cggtcaaacc gcagcacgac aaaaaacaga
     2461 tcgcggagac ggtcgcggcg tgtctgggtc tggaagcgga tgaggtatac gcgcaatttg
     2521 cccgcaccga ctcgcggtac atcaagctgg gggtttcgct caacgacaag gtgttcgacc
     2581 tgctcgcgga tccggagacg atcagcggga tcggttccga agatctggtc tgccgctcct
     2641 atccgcaggg tcgccggatg tcgcacgttc tggggttcgt caatgctatg ggggtgggcg
     2701 gtgccgggat cgagcagcag tacaaccgtt acctgaccgg cacacccggt ttgatcgagg
     2761 gcgagaagga tgccggccgc cgcgaaatct ggagtcggcg cacggtgcat gtcccggcta
     2821 ttgcgggctc ggacctctat ctgacgctgg atcataacat ccaatatgag gtcgagttgg
     2881 cgctccgcga ggtggtggag aagtttgagg ccgcgggcgg gtgggtcatt gtgcaacggg
     2941 tccgcaccgg cgagattttg gccatggcct ccttccccga tttcgcgccc gaacagtata
     3001 ccgaggagag cgtcgatgtc tggcgcaact cggctctggc aatcgtctac gagccgggtt
     3061 cgatcatgaa ggcgatcacg gtggcggcgg cgctcaacga acgcatcgtg acgccggaaa
     3121 cagtgattga tgcgggggag gggtgttgga tgtacgcggg caaaccgttg cgtgatcatg
     3181 ttcacggacg ggtcaccgtc agcaccgccc tgaaaaaatc gagcaacatc gcgtgcgcga
     3241 aaatcggttt gatgctgggc aacaaacggc tcgagaccta tctgcgggcc ttcaatttcg
     3301 gtgaaaaact gggaatcgag ctgccgggtg aggaacgggg tattctcgcc cgggcccgtg
     3361 attgggacaa actcaaaccc acgcgtatcc cgatcggtca gggtgtggcc gttaccggat
     3421 tgcagatgat ctcagcctat tcgacgctgg ccaatgacgg cgtgatgatg aagccgtatg
     3481 tggtgcggcg gatcgtctct cccagcggcg agacgatttt tgaggccaag cccgaagtgt
     3541 tggggcggcc ggtgcgtccc gaggtcgcgc gcgctgtacg cgagatgttg cgcggggtta
     3601 ccgaggaggg gggcacgggg aaacgggcct ccgtgccggg atattcgatc gggggtaaaa
     3661 ccggcacggc gcagaaggcg gtgccgggcg gatactccag cacggattat tacgcttcgt
     3721 tcgtggggtt cgtacccgca ggggatccgg tcttcagcgt gctggtttct gtggagcggc
     3781 cgcggcccca gcacaccggg ggctttgttt cggcgcccgc tttcgccaag atcgcggcgg
     3841 cgaccgcccg gtatctcgaa gtccctcccg accagcctgc cgaggaaacg gagcaagtgg
     3901 cgaatcaccc gtagcagacg gcacgaactt gcgccgtgga gaagttttca tgaaaatttc
     3961 ggaaattcca gaactcgcgc ccctactcgt ttccgaggca ccgaccgtgg cgttcaccgg
     4021 aatccagtgc gattcgcgcc ggatccggcc cggtgatttg tttgctgcga tcaccggcac
     4081 gcgtgatgcg ggcggacgtt acgccggtgc cgcgctggcg aaaggcgcgg tggcgatcct
     4141 ttcggaaacg ccgctgccgg agtatcaccg ccaggtcatt ctggtccggg acgtgcgcct
     4201 cgcgttggcc tgtatcgcgt cggcgctgaa agcgtggccg tcccgtaaca tgcgggtgta
     4261 cgggattacc gggacgaacg gcaaaaccac caccgcgtgg ctgttgcgcg aaatgttggt
     4321 ggccggaggg gccgtgcccg gattgctgac caccgttcag gtcgagtatg ccggtcgcgc
     4381 gatccctgcc acgcgcacca caccggacgc ttgtgagttg caggccattt tggccgccat
     4441 gcgcacgagt ggttgtgaca ccgcagtgat ggaggtgtcg tcgcacgcgc tggatcagca
     4501 gcgtgtggcc tggacacggt tcgcgggcgc ggccttcacc aatctgtcgc gggatcatct
     4561 cgattaccac ggcacgatgg aggcgtattt cgtcgccaag tgcaagcttt tcgaccaatt
     4621 ggccgctgaa acgccgggtg cgctcgccgt gtgcgtggca gatgccggat atggggatcg
     4681 catgaccgcc tatctcgcca cgctaccgtt acggcgcctc acctgcgggt tcacgcaagc
     4741 ggccgatctg cgggccgaag ccctggccgt tacccccgac gccagtacgt tcacattggc
     4801 ggcaccggat ggacgccgcc ttgcattacg cgtgtgcctc gcgggtcggt acaacgtggc
     4861 caacatgctg tgcgcggccg gtttggcgtt ggaagccgga gtgccgttgg agaaggtggg
     4921 agagacgttg gaggccgccc ggccgtgttg gggccggctg gaacgtgtgc cggtcgatct
     4981 gcccgccacg gtgtttgtgg actatgccca taccgatgac gcgctcacga atgttctgga
     5041 gaccttgcgc gaggtgacgc gcggacgcct gagggtggtc ttcggatgcg gcggcgaacg
     5101 cgaccgtgcc aagcgtccgc tgatggggcg cgcgtgcgcc gctctggcgg atgatctggt
     5161 gatcacctcc gacaatccgc gttcggagga tccgcgggcg atcattgagg aaattctgca
     5221 gggcataccg gaagggacct cgtacgaggt tgaggcagac cgccggcttg ccatccgacg
     5281 cgcgttgagt caggcgcgcg agggggatgt ggtgcttatc gccggcaaag ggcatgagtc
     5341 atttcaggaa ctcgcgggcc gcacgctgcc ctttgatgac cggcaggtgg tggcggagga
     5401 ggcgcgaata ctcagcgagg cgcgacagca ggatcggtag ggtggacaat cgggacgggc
     5461 acgaagatgt cgaacaagca gccacagagg ttggatgcgg ccgcgtttgc cgcatggacg
     5521 ggcggtgacg cgcaggcgtt gccgtcaacg ttctgcggcg tgacgcagga ttcgcgcagg
     5581 ataacgccag gctgcctgta tgtggcgttg cgcggggaac ggtttgacgg gcatgatttt
     5641 gtggcacagg ccttgcgcga cggtgccgcg gccgcgttgg tcgagacggc ctggtccgcg
     5701 ccgtccggat cgtccggctg gcccctgatt cgcgtgaagg acacccgcca agcgttggct
     5761 gatgccgcgc gcgcgtggcg gcgtttgaac cgcgcgttca ttttgggcat caccggcagt
     5821 tctggcaaaa ccacgaccaa agagatggcg gccgcgctgt ttgcagcagg cgggcgcgtc
     5881 tgcgccacac gtgggaatct gaacaacgag gtggggttgc cgctgagtct gctcgccatg
     5941 ccgccggaaa ccgagtacgg catctttgag ttgggctcca accatcccgg ggagatcggg
     6001 cggctgagtg acacacttca accatgcgcg gcggtgatca ccagcatcgg atgcgcgcac
     6061 attgaacact tcggcacgca ggaggcgatc gtgcgggaga agggggcgct gttgcgcgcg
     6121 ttgccgcacg gggggttcgc cgtcctgaac cgggagatta cgggatttgc gcaactggcg
     6181 gaacagtccg gcgcgccggt ggtgacggtt acgctgacgg gagggggggc ggatttctcg
     6241 gccgaggcga cggatctcca gaccggcgcg ctgcgaatca cggaacaggc gaccggcgaa
     6301 gagacgcgac tatgcagcgg actgccgggt gcgcacaatg cgtcgaatct gctgctggct
     6361 ttcgcggcgg cgcggcgtgc cggactgccc gcctgcgcgt tggcgcaggc cctgtcgggt
     6421 ctgacgctac ccaagatgcg ctgggaggtg atggtgcgcg atggcgtgac gtgggtcaac
     6481 gatgcttata acgccaatcc ccagagcatg gccgcggcac tcaaaacgtt tgcggaactg
     6541 ccctgccggg gacggcgcat cgtggtgttg ggcgatatgc tggaactggg tgaacacacc
     6601 gaggcgttac atcgcgaggt cggccgttgt gtggcacaga cggctccgga cagactgttg
     6661 acagtgggtc acgacgcgaa gctgtatttg gcggaggagg cggtgcgggc gggttttccg
     6721 cagacgaagg tggtctgtgc ggacgatgcg cgggcagcgg gtatcgtgtt gtgcgagtgg
     6781 atacggagcg gtgacagtgt tttgctcaaa gcgtcgcgcg gcatgcggct ggaagggatt
     6841 gtgggccatg agcaagtatg acggtgtaca ggctctggtg ctgggcgcgg gaatcagcgg
     6901 tgaggcggcc gcgagactgt tgctcgcccg cggcgggcgt gtgcgggtga ttgacgaggg
     6961 gaaagcggac gcaagcgccg cagtggcgca acggatcacc gcagccggag gtcaggtgac
     7021 gttcggggcc gccggtctgc cgtctgaacc gttcgacgtg tgtgtcgtga gtcccgcatt
     7081 cgccctgaac catccgtgga ttaccgcatg ccgtgcgcgc ggattgccga tggtgcccga
     7141 actggaactc gggagcgaat actggtccgg gcgcgtgatc gccataaccg gaagcaaagg
     7201 caaaagttcg ttggtcaagt ggtgttcgga cacgctgacg ggcgcgggga tccccgcctc
     7261 gcctgcgggc aattacggga tcccgttgtg ccaactcgcg ctcgaccggc ccgacctgct
     7321 ctgggcggtg accgaggtca gttcatttca gatggagcat acccggacct ttcatccgga
     7381 gatcgcgatc ctgctgaatc ttcaggccga ccatcaggat cggcatgtgg acatgacgga
     7441 atacaagagc gccaaaatgc ggctctttgc gtcgatgtgt ccgggggatg tcgtgctgct
     7501 gccggccggg ttcgaggacg acgggtgcgt gcctccgggg gtcacgctgt ggcggttcgg
     7561 tacggatgtg ggatgcgatt ggcgttatgc ggatcatgcg gtgacggggc aggcggaggg
     7621 tacgcgtgtc cgggtggaga ccggggggtc gtggttcgac aacgaagtgt tcggactttc
     7681 gctgtcggcg ggcgtggcgg cattgcatgc cgcagggctg gatgcggagc agatcgcggc
     7741 gggagtgcgg gcgttcgagc cgctgccgca ccgtatgcaa ctcgtgtggg agagccgaca
     7801 cggcgtccgc tatgtggatg actccaaagc gaccagtctt gcggccacgg ccgcggcact
     7861 gagaatggcg cgcaaaccgg tacgcctgat cgcgggcggg cttttaaaag agcccgatct
     7921 cgaagggata aaagagttgc tgacaaaaac cacaaaaaaa ggttatctta ttgggcactg
     7981 ttcaaggcag atgtttcagg cctggtcgga tgtgatccct tgcgaagagt gcgggaccct
     8041 ggatcaggct gtggcggcgg cggcgcgtga agcccgggcg ggtgacaccg tgctgctttc
     8101 gccggggaca gcgagtttcg accagttttc aagctatcgg gaaaggggag aacgtttcac
     8161 ggcacaggtt cgcgcggttg cggatctctg agcgtttctt ttctgagggc aagcaagaag
     8221 gataaggaga cacaatgaat cgtcaagtca tgcgtgttgt tgcaggcgtc aacctgattg
     8281 tgggttgctt cctgatgcag ggctgcggta tgtttaaccg gaagagtgac accacttcga
     8341 ccactctgcc tgacggggaa ctgccaccgg tggaggtgca gccgttggat acaactcccc
     8401 cgcctgttca acctgtcgaa acctcgttgg tcgagttgcc gtcggtgcag acttcgaccc
     8461 cctacacgat tcagaagggc gacacccttt ccgccatcgc gtacaagtac aatgtgcgct
     8521 ggcaggacat cgtggccatc aaccccggca tcgtgcctaa ccggctgcgc atcggacagg
     8581 tgatccagct ccccggtcag gtggacctct ccagggcgcg ctccgtgccg gtggcgacac
     8641 ccagcgtcag ggctcccaag acggtggcgc cgaaagcggc cgcagccgtg ccgggtccga
     8701 cgatcacgta tgtggtgaag cccggcgact ctctctcggt catcgcgcac aaacacggaa
     8761 tcaagactgc ggccttgcgc gaggccaacg ggttgaagag cgacaaaatc atcgtcggtc
     8821 agaagctgaa ggtgcccggt gccgtcaaga caccgtccgc cgcaccggtg acgaagaaga
     8881 gcgccgcttc ggtgcaggct cccaaaaccg tcgcgccgaa accggcggcc gccgtcaaga
     8941 cagaaccggc cccggccgtg acgaccacgc cgccgcccgc tgccgagaaa acaacggcgc
     9001 ccgccgcggt tgaatccgct ccggtgaaaa tcgagaccgc gccgccgccg gccgctccgg
     9061 ctgcggagtc ttccacgcag acctacaccg tgaaagaggg tgaggatctg tacgctgtgg
     9121 cgatccgctg gggtgtgagc ccgagcgatc tcaaggcgct caacaacctc acctcaagcg
     9181 aattgaaggc cgggaccgtg ttgaagatcc cgaacgcggg acagtaacgg agacggacgg
     9241 catcccatgc gcagcactct gacggtcttg tcgttgacag tggtcgcgtt gctggggttg
     9301 ggaattgtcg tgttggctac ggctagtgcg gtgcgggctc agggtttata cgctgacccg
     9361 cactttttcg tccggcgcca actgatctgg ctcggtctcg cggcggtgac ggtgtttttg
     9421 atcaaccact tcgattatca ttggtggcgg aatacgccgt tgctgaccgt cggcttttat
     9481 ctgctggtgg tgctggcgtt gttgctggtc ttcgtccccg gtatcggcgg caaggtcaag
     9541 ggcagctacc gttggctcaa cctcggcccg ttccgcatgc agcctagcga gttcgccaaa
     9601 atcctgacgg tcattgggat atcggtctgg atggaccgca tcgggtggcg ggtgcgtcaa
     9661 ttcacccaag gcgcgctctt gcccggatgc ggattggcat tgcttgtggt tttgctggtg
     9721 atggagccgg acttcggctc cacgctggtg gtgtgcgtgg tggggggggc gctgatgtac
     9781 gtggccggta cgcgattcgt gtacctgctg ctgttcggcg gactcggtct ggcgggggtg
     9841 ggcgcttttc tggtcacaaa cgctaaccgc atgcgccgca tcgcggcctg gctccccgaa
     9901 cagttccgtt tgataatcga gagatggttg ccgcccggac acacggagga gggtgcctcc
     9961 gccgccaaat atcagttgga gcaggcgatt ctggccttta agaacggtgg tccgtccggc
    10021 gtggggttca atcagagtat tcagaaatat aactatctgc cggaggccca caccgacttc
    10081 atcttcgcga tcggcggcga ggagttgggg ctggcgtttt ccctgttcgt ggtggcggcg
    10141 tataccgtga tcctgttctg cggcatccgg atcgcgttac gtgcggcgga ccggctcggc
    10201 cggatgctgg cgttcggcat gacgctcctg ctggtgtttc aggcggcgtt caacatcggc
    10261 gtggtcacag ggtgcctgcc caccaaagga ctggccctgc ctttcatgag ctatggggga
    10321 accaatctca tcacggcgtt gtttgcggtc ggcacgctgt tgaacgtggg gcgccatgtg
    10381 gatgtgtttg acgagcgcat gcatacgcag gtggtgcgta acgccgcgca agaggtgtaa
    10441 ggcgggactg cggggttccg gtcgggggaa ttcaaagggg ggaagagtgc cgggacggaa
    10501 tgtgcggcgc gtcatggcgc ggggaatcct gctggcggca ctgtgggccg cagcaggagg
    10561 gtgccgcgcg ttgcgcgagc cggaatcggg tccgctgccg atacgttgct acggggagga
    10621 ggccgcgcgg ttgcgcggtc tgccgttgac acgcgagatc ccggtgacga gtgaggcgcg
    10681 cgacgtctgg atggcggtgc tggagggcga actcgataaa ccggagaacc ggcggtttct
    10741 ggaagagacc gagcggctcg cacggcattt ccgtgtcctc ccggccgatg tgtcgctgcg
    10801 cgcgctctac cgcgcgctga tgcgcgagca ggtggccgcc tattacgatc ccgagaaaaa
    10861 acgtatcgca gtcgttgagg tgccggggtc cggctatccg gagggcgcgg caaaccgggc
    10921 gctggtcgac cgcttcgtgt acacgcatga gtattgccac gcggtcgagg atgtgcattt
    10981 cgaccttgag cggttgaccc gggcggcact cagcgacttg gacgccaacc tcgccttgac
    11041 ttcactggtc gagggtaacg cggtgctggt cggtgcggac aatctgtgcg cggcatggcc
    11101 ggtcaacacc gcaacaccct tgggggcttt tctggtttcg cttgtcagcc ggctggaact
    11161 cacggatacg gttggtgccg gtctggacgc ctacccggcg tttctggccg gggcactggt
    11221 gcggccgtat ctggacggtg ccgttttttc gaatcgtttg cggcgcgagg cgggatggca
    11281 ggcgttggac cgggtttacc gcgaacgcct gccggtcacg accgccgaga tcctctatcc
    11341 ggaacggcgt tacctgcggc cgttccagcc tgcggagatc tcgctcgcgt ctgatgtgat
    11401 gggacagggc ccggatgtga tgcaaccggt ttcgaccaac cggctcggtg ttttggggac
    11461 ggcgttgtgg ttaggcgggg aacggctcgc gtcggtccgg gatttcggct ttctgcaggg
    11521 gtggttgggc gaccgtgtct ttctcgcgga agcgccggac gggacaacat gcacgatctg
    11581 gctgagctat tgggagcgac cggggcatgc gcgcgcgttc cggaaacagg tgttacgacg
    11641 gctggaatcc gacgcgttta caggcgtgct tcatgccgtg cggcgcgagg ggcgtttggt
    11701 ggcggcggtt tggatgagcg gtcccggcgc ggaggaggcg gtgtgcgggg agaaggcatc
    11761 gcgtgccctg gcgtcgcggg tggaagcgga aacgccgtca tggtttgcct cgtggtggga
    11821 ggatctgcct agtccggtga gcgtcggccc ctccgggtgc ggagtgttgg gcgggtgggc
    11881 cgcggagttg ctcggcggca cggggttctt tcatgtccgc ctcgcctgcg gggggctgtt
    11941 acgcgttgaa cacacgccgg accggcacta ttggggcacg gccttcgggt tgctgcgcca
    12001 tgtgggggac cgccgttcag acttcacatt ctggcagatt ccgttggtgg cctcgtggca
    12061 cgctcagggg caaggggaaa ggcggcgttt ccgttggtca gtcggggccg gtcttgtggc
    12121 cacgggagaa ccggaccgca tacgcgtgct gaggatcccg gtgtggcgtt cggcgacggc
    12181 cgggaagaat ataccgtgaa gtcgtcattg tcgtaagggc ggcgttttag tataacgtca
    12241 gcctcacgca tccgaaccgt tcacggtcag ttcgactcgt ggtgtccgat gtgtgaagaa
    12301 gaggagatag agtatggaga gtagttcgta taacccgttt caggtggcgc aatcgcagtt
    12361 tgataaggtg gctgcggtac tggaattaga cgggggtgtg cgtgatctgt tgcgtcagcc
    12421 gatgcgggaa taccagttct ccattccggt tcggatggat gacgggaccg tgcgcgtgtt
    12481 taagggtttc cgggttcagc acaatgatgc gcgggggcct gcgaaaggcg gcatccggtt
    12541 tcatccaatg gagacggtcg atacggtgcg cgcgctgtcg atgtggatga cgtggaagtg
    12601 ttcggtggtg gatattccgc tgggaggcgg caagggcggg gtggtttgcg atccgcacca
    12661 cctgagtctg cgagagcagg agcagatctg ccgcgggtgg atccgtcagg tcgttaggaa
    12721 tctcggaccg gtgagcgacg tgcccgctcc ggatgtgatg accaacgccc agcacatgct
    12781 ctggatgctc gacgagttcg agacgattcg cggcggccgt taccccggct tcatcacggg
    12841 caagccggtc ggcatgggcg ggtcgctcgg gcgcacggag gccacgggct tcggtgtggt
    12901 gttcacgttg cgcgaggcgt tacgggaact cggcatcgaa ttgcggaaga ccactgccag
    12961 cgtgcaggga ttcgggaacg tggcgcaaca cgccgtgcag ttgtacaccg aactcggcgg
    13021 tacggttgtc gcgatctcct gttgggacca gacggacaag acctcctaca ccttccgcaa
    13081 ggcggacggg gtctgcctca aggagttgcg cggcatttct gacaagtttg gcgggatcga
    13141 caagaaccgc gcgcgtgaac tcgggtatga gatgctgccg ggtggagcct ggctggaaca
    13201 ggaagtggat attctgattc ccgcggcgct ggagaaccag gtcaaccgcg acacggtcca
    13261 gaagatctcg aagagcgtcc gggtcatcgc cgaaggcgcg aacgggccga ccacaccgga
    13321 ggcggatgag gtgctccaac aacgcggcat ctttgtgttg ccggactttc tgaccaacgc
    13381 gggcggggtc acttgcagtt attttgagca ggtgcagtgc aacaccaact acttctggga
    13441 gaaggatgag gtgcttgcta aactggacac gaaaatgact tccgcgttcg cggcggtcag
    13501 tgatctggcc cgccgtaaga aactctacct gcgcgacgcc gcgtacatga tcgcgatcaa
    13561 ccgggtggcc gaggcggttc gcctgcgcgg ctgggcgtga ggcggaccgg ataggcagcg
    13621 aaaaaaagtg atttttcttc gccgcacggg ttgacatcgt gcggttcatt ctgctactat
    13681 tcccatccgc gccaacaacc ggaaggacat ccggtcggcg tattgatcta tgatatccgg
    13741 aagctcaggc gcagacccga cggatgccga tccggagggg atgcgggaag aagatgagga
    13801 agcttgtgtc ggaatgcgaa cgcgaaagaa acgcgccgcg gcctttcgtg gctgtcggcg
    13861 cgcgacggat ttttcttgag agtttgattc tggctcagaa cgaacgctgg cagcgtggat
    13921 taggcatgca agtcgaacgg gatccgggag gcagcaatgt tttccggtga gagtggcgga
    13981 agggcgagga acgcatgggc aacctgtccc ccgggtgggg ataacgccgc gaaagcggcg
    14041 ctaataccga atggggcggg tctggcgatt gccgggccgg ttaaaggcgg ggaccccgcg
    14101 cgagcggggc ccgccccccg gggaggggcc cgtgtgccat cagcttgttg gcggggtaac
    14161 ggcccaccaa ggcttacggc tagctggtct gagaggacga ccagccgcac tgggactgag
    14221 acactgccca
//
LOCUS       DAOSLE010000069        14063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4429_length_14063_cov_6.996573, whole genome shotgun sequence.
ACCESSION   DAOSLE010000069 DAOSLE010000000
VERSION     DAOSLE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14063
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4429_length_14063_cov_6.996573"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1275
                     /locus_tag="PK576_06170"
     CDS             64..1275
                     /locus_tag="PK576_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61215.1"
                     /translation="MRKMIWFLFLLCAAALPWESACRAAEAPPVITVVKKGAEKNTVS
                     LSRLNASGPYGKLFVRTLTRDLELSGWFKVASSGAVQVQGTVADAGSGVQSACRVTWP
                     GKAFNWNRVSMGQAEVRRQAHQLADEMVRLITGEIGIAQSRIVFVNRRGRNNADLYLC
                     DADGQNLMQITHDNVAAVGPRWAPNSQDIYYTSFLKGYPAVYRIGVGRGERKTLAAFK
                     GLNTGAAISPDGSRAAIILSYQGNPELYVLHLTSGQLTRLTQTVHAAEASPCWSPDGR
                     QIVYVSDPAKTPQLYLVDPLSRQSRRLTYKGSENVNPDWSAKGKIVYATRRGGGGYQI
                     AVLDPRQGEASCELLTQPGGYEHPSWAPNSRHVVCSNRSTLYILDTLGDPAVRLINLA
                     GNWMSPDWSSR"
     gene            1297..1860
                     /locus_tag="PK576_06175"
     CDS             1297..1860
                     /locus_tag="PK576_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE61216.1"
                     /translation="MHIRSIVAVNVVLMGLLLFTDTGCRLPWQKGKGKGGSAGLGDPT
                     LLENADLAQVTDEFGHTLSGARFEDTLQPVQGVTFAPVYFAFDSYALPPTEISKIDQV
                     GQHLHQNAGHVLVVEGHCDERGSNEYNLSLGENRAQALRAHLVNQGISADRIQTRSYG
                     EEKPAVAGSGEEVWRLNRRGEFALFQK"
     gene            1888..2343
                     /locus_tag="PK576_06180"
     CDS             1888..2343
                     /locus_tag="PK576_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOE61217.1"
                     /translation="MSFFPSVAFLGGSVGTGEWIVLFVVVLIVVGPKRLPEIARKLGR
                     TMEMFRRAADEFKDQLLNMDREPAEATPPPETDAAYDPDGYGVPSDPAEPYDGTPYPD
                     TGEYADATDVPGEATPVSDGIPETDGSAGDAAAEPAASSKPEQPTEDVS"
     gene            2340..3152
                     /gene="tatC"
                     /locus_tag="PK576_06185"
     CDS             2340..3152
                     /gene="tatC"
                     /locus_tag="PK576_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOE61218.1"
                     /translation="MKVGRFSLPKFGQGDEYNDPPRPFLEHLIDLRDCLIHCAIAWVL
                     CEILIVPFAPRITNWLIAPAGVSKDMVQGLGWTAGFSILLKIMLWGGTALSLPFLLFF
                     IMRFVFPGLKRSERSIIIFCLFTSSLLFAIGVWMAYAATLEIAIGVLQKINAWVGIKV
                     DIVRLDEHVDIVIKTIIAFGLAFQLPLLLLVLGWIGLVPSEALRSRRRIAIVVIFVIA
                     MILTPPDPVSQILMAVPMCALYEACIWIIRLRELARGDEKDDEPSAPDNDAD"
     gene            3171..3761
                     /gene="hisB"
                     /locus_tag="PK576_06190"
     CDS             3171..3761
                     /gene="hisB"
                     /locus_tag="PK576_06190"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002652424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOE61219.1"
                     /translation="MARTAARTRTTRETDIAVTLNLDGSGVSRIETGVGFFDHMLELL
                     ARHALVDLEVRAKGDVHVDYHHTVEDVGLVLGACLNEALGERRGIRRYGFALLPMDEA
                     LCEVVIDLGGRPFLVFASPMKHMRVRDFEMQLLEEFFRAVTVEGRLNLHVRQVYGDET
                     HHVCEAIFKGFARALRMAVEPDPRETGIPSSKGTIA"
     gene            3758..4480
                     /gene="hisA"
                     /locus_tag="PK576_06195"
     CDS             3758..4480
                     /gene="hisA"
                     /locus_tag="PK576_06195"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011127638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOE61220.1"
                     /translation="MIILPAIDLKDGKCVRLRQGRAEEVTVYSDDPVAQAREWLAQGA
                     EQLHVVDLDGAFQGEPQHMEQIAQIVRAVGIPVEVGGGLRTDAQIEQMLGVGVARAIL
                     GTRAVESLDTLRALARRFGAAIAVGIDARDGFVQVRGWVETTKVRAVDLARRVEDAGV
                     RTIIYTDTATDGMLGGPNLTALREMCATVACRVIASGGVSAPEHVAALRDLGFPNLYG
                     AIVGKALYDGKTTLAAMRAAAC"
     gene            4489..5778
                     /locus_tag="PK576_06200"
     CDS             4489..5778
                     /locus_tag="PK576_06200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE61221.1"
                     /translation="MLGKTKITRLKNGVSVVTSAIENAESIAMGIWIGVGGRHERRPL
                     AGVSHFLEHMLFKGTPTRSALAVSQAIEGRGGYLNAFTQEEATCYYARLPYEYLPQAF
                     DVLSDMYLHAAIREEDFRRERDVILEEIKMYQDLPQHVVQENLLEAMFRDHALGAPLS
                     GNPKSLGAMSRETLLRYKARAYTPGSTVFAFAGRLDHEACVARVERAVGGQPRRRGLD
                     FMRVDGRVPQRRLSLTRKEIAQTHAVIGFRIFGRHDDRRYALRVLNGVLGENMSSRLF
                     QSVRERRGLCYSINSGFQLFEETGLFTVSGGFDARRTIAALKRTAQEMRRLIDAPVGA
                     RELRRVKEYLQGTFRLGLEGVGNQMMFIGESLLNYGRVVCPEETLAGIAAVTADDVQR
                     VAEEVFEPSRLTLSLVVPNAQAESEDEWMSSLGALFA"
     gene            5806..8493
                     /locus_tag="PK576_06205"
     CDS             5806..8493
                     /locus_tag="PK576_06205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE61222.1"
                     /translation="MRKAGQGSETPLRVPPDENWRTTDQDEINRRKRRAASEPMTILN
                     RTPAHPVFSNFNVTSRSSGQTYQVEVREISPRVGSCTCPDFRVNDLGTCKHLERVIRE
                     LACGEPLSDRIDVVPDRVMGRLLIERNLNRLPAALRPYFDITGLALPEFSPTEILEAC
                     ERVGLDVVRVSQDVEPWMARLARQTERIRLRRAYEHAVCSGHYPPQETLEPLLPYQRE
                     GMLHLAFTGRALLADEMGLGKAVQAVAAVALLYRLGEVRHAVAVVPEAHQREWEGHIR
                     RYTALSCETVAGDRAARRRAYEQKAAFTLVSYEQVLRDSRLFLDVLNPECVILDEAQR
                     IRNWNTRTAQTLKQIESRYLFVLTGAPLEQRLDELYTLMSLIDPEVLGPLFRFNREFY
                     TLDEDGRPTGYQNLHRLRERIRPYVLQRRRTEVETELPDRTERVVYVPMAPEQRDRYA
                     THETQATLLAAQSQQRALTRSETERLRREIAMMRMLCDTPYITDETSRVCPKLEELRN
                     AMESVLAMSGVKVLVCSEWERMLQLVRALCEQQGWGYAWHVGSVPQGQRKREIRRFRN
                     EPACRVFLATDAGCPAGSLSQASVVIQCDVPWTAARFEQRIARVCHSHQVRSVTVLNL
                     VAADSIEARLLEQLKQGRANGAAVGARVSLTQRLATLLPLTEATERTPVEHPSFEALR
                     ALDPELGFAAALAERMGEKLVACDVWHTEEGRPVFLLAAEGPLRVANLIADRLAADWF
                     AHVAAGPVLEVVAPGVSDVLRRFMDKGWLRMPHPVGARTLYQRGQGLSRPRMMPDDEQ
                     ARILELREQAGQAFRQARRLMRQLAFDAARAPLELSMVLLARAFARQHRLAEPRHARQ
                     TLTEPYAELWGDLLPVLREGLQENSPVALSRALMAKFGR"
     gene            complement(8536..9861)
                     /gene="aroA"
                     /locus_tag="PK576_06210"
     CDS             complement(8536..9861)
                     /gene="aroA"
                     /locus_tag="PK576_06210"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOE61223.1"
                     /translation="MQHSLTLSFSADRPLTGEAVLPGDKSLSHRAALFAALAEGVSVV
                     DRFLVSGVTRAMLNALTALGVAWRLEENRLTVPGVGLHGFTPPTAPLDCGNSATTIRM
                     LTGALAAAGTPCVLDGSEGLRKRPMDRITEPLRAMGVRISTPPNHCAPITLAARSPDV
                     PLRAQAYTLPVASAQVKSCLILAALAADGETVLREPGPSRDHTERLLASMGADIRVCG
                     PEVRVTPLARPLAPLALTLPGDISSAAFLLVAAALTPGSTILLRDVGLNPTRTGILDA
                     LREMGADLTVENPQTAAGEPVGDLRLTAAPLQAVRVEGERVVRMIDEFPVFAIAACFA
                     RGVTEVRDAEELRYKETDRIAVLCAELRALGARVEERRDGFMIHGGSLRGGVCEAHGD
                     HRLAMSLALAGLAAPEPVSVRHAEILNESFPDFVTQLRALGATVAEIRG"
     gene            complement(9878..13678)
                     /locus_tag="PK576_06215"
     CDS             complement(9878..13678)
                     /locus_tag="PK576_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOE61224.1"
                     /translation="MNRSFFLFALFPFFPYVSGAEVLPEAFPLDRQSVMTVAASVTAE
                     RFPNADRVLVDNRVLERYERDGTSVVWDDEYNKILTEKGRRDASSVRIPFNAHYGTAL
                     VYRAEVIKPDGRTVALDIASYARVVVESEQMGANIYDPNNKILNLSLPGLEIGDVCHL
                     ITCRKTVKVRMPDTWADYAVFEYDSPIVRLEYAISAPPELPFRHALLRAPVPGTVMKT
                     ERREAEGRTLHLWTVTNVPQMYPEPDMPPLHTQVQRLLLSTIPDWRTVSRWYWELCAP
                     ALAATNTAMRTRVDALTAGSASRDETIRRIFKFVSQEIRYMGITTEDTAPGYEPHPVS
                     LTFDNRYGVCRDKAALLVALLRMAGIDAYPVLIHAGAKMDPDVPMPFFNHAVTAVDNP
                     DGGYLLMDPTDENTRDLFPAYLCNRSYLVARPEGETLRVSDVYPVEKNLTRINTEGTM
                     DRNGSLLLKTRIRFEGINDNAYRSYLVRQKPEQRRNFFEAALKNRLPGAEVIECMIAP
                     EDLRDTETPLAVTLTTRVPEFPVRGEGLDLLAMPWLGTGLGYANFVIGQTGLDKRRYP
                     LETGITCGTEERIALHAAAGLGAPHTLPGAVHIDRAGVFFAMSQSVSNSVLSGSLTYH
                     LKTPEFSPSDYLELKSILKEIEFAGRKRPLFEAGDPNPPDHETLHDLTEAHLVSPHAW
                     TTTHTWSRRILTYAGKKSCSELKIPFNPVWQQIDLLAAVVSNANGIVHTVTPKEINQM
                     DAAWTGSAPRYPAGKLLVVNLPGVETGSVITVSTRFTQTNAPFYCHTHTFGGTAPVAE
                     RVYRLTYPDTLSPAIRLVNPGSVTMDSETRDGLVTHTWHAPQQPVTRTEELLPPWHFF
                     RPVLFVSFGDWSEYMRQLRRAAETAAETDREARRRAKERVRGLRDPRERLLAIRDEVL
                     RTIRPAGPAFFDLPAECLSPPDRTLADQYGHPADRALLLRAMLRAVGFDAELLFAGQD
                     TTCYPAFSQPMREIPQIEFYRHPLVAVKLRDGICYLNEGDQYSELGSSELDGAPALTL
                     KGRPVTIELDEPLRNRSHHAWTIDLDVSGAARITVSNRFFGTQAGTFRKQYREMLPEE
                     RRRHHLELVSKIARAAEPASALITETEGYPCLRTYTVTAARYATLEGRTLTLLIPEVA
                     GVLFPLRADSRVNPLFLGMRDRSDLTCEILLPPGYTRLPVLPESKHWILPNGLGTLDY
                     SVKHITRADGRVQISITRALVRQSAELPAELYPALLEYNRRLAHPATRTLVAERPTNL
                     YPTP"
BASE COUNT         2536 a         3959 c         4851 g         2717 t
ORIGIN      
        1 gcgatgtctg cgcttgctgt tggcgagggg acggggtata ctcgtaacac attgggagtt
       61 tttatgcgca agatgatttg gtttttgttt ctgttgtgcg ccgcggcgtt gccgtgggaa
      121 agcgcttgtc gggcggcgga ggcaccgccg gtgatcacgg ttgtgaagaa aggcgccgag
      181 aaaaacacgg tgtcgctgag ccgcctgaat gcgtccggac cttacggaaa actgttcgtc
      241 cggaccttga cgcgcgatct ggaattgtcc ggatggttca aagtcgcgtc gtccggtgcg
      301 gtgcaggtgc aggggacggt agcggacgcg ggcagcggtg tgcagagcgc gtgccgggtg
      361 acatggccgg ggaaggcgtt caactggaac cgggtgtcga tggggcaggc cgaggtgcgg
      421 cgtcaggccc accagttggc ggacgagatg gtacggctga tcacgggaga gatcggcatc
      481 gcgcaatcgc ggatcgtgtt cgtcaaccgg cggggacgca acaatgccga cctgtacctg
      541 tgcgatgcgg acgggcagaa tctgatgcag atcacgcacg ataatgttgc ggcggtcggc
      601 ccgcgctggg cacccaacag ccaggacatt tattacacga gctttctgaa ggggtatccg
      661 gcggtttacc gtatcggggt ggggcgcggg gaacgcaaaa cattggcggc gttcaaggga
      721 ctgaacaccg gggcggcaat ttcgccggac ggttcgcgtg ccgcgatcat cctttcgtat
      781 cagggcaacc ctgaactgta cgttctgcat cttacttcgg gccagctcac ccggttgacc
      841 caaacggtgc acgctgcgga ggccagcccc tgctggtcac cggacggccg ccagatcgtt
      901 tacgtgagcg acccggccaa gaccccgcaa ctgtatttgg ttgatccgct ttcacggcag
      961 tcgcgccgtc tgacctacaa agggtctgaa aacgtcaacc ccgactggag cgccaagggg
     1021 aagatcgttt acgcgacccg ccgcggtggg ggggggtatc agatcgcggt gctcgatccc
     1081 cggcagggcg aggcctcctg tgagttgctg acgcagcccg gcggatatga gcatccctct
     1141 tgggcaccga acagtcgcca tgtggtctgc tccaatcgtt ccactctcta cattcttgac
     1201 actctggggg atcctgcggt acgtttaatc aaccttgccg gaaactggat gtccccggat
     1261 tggtcgagtc gttaaagggt tcagaggagt acacgtatgc atattcgttc aatcgtcgcc
     1321 gtcaacgtcg tgctgatggg tctgttgttg ttcacggata ccggttgccg tttgccctgg
     1381 caaaagggca aaggcaaggg aggctccgca gggttgggtg accccactct gttggagaat
     1441 gccgatctcg cgcaggtgac cgacgaattc ggtcatacgc tgagcggagc gcgttttgag
     1501 gacacgctgc aaccggtgca gggcgtgacg ttcgcgccgg tctatttcgc gttcgatagt
     1561 tatgcgctgc cgcctacgga gatctccaag atcgatcagg tcggccagca tctgcatcag
     1621 aacgccggtc atgtgctggt ggttgagggg cactgcgacg agcgtggcag caacgagtac
     1681 aacctgtcgc tcggtgagaa ccgcgcacag gccctccgcg cgcatctcgt caatcagggc
     1741 atttcagccg accggatcca gactcgcagc tatggcgagg aaaagcctgc ggtcgccgga
     1801 tctggagaag aggtgtggcg cctgaatcgt cggggcgagt tcgcgctgtt ccagaaatag
     1861 ggcgttgcgg gtcgggggct tccgggcatg tcgttttttc ccagtgtggc gttcctcggc
     1921 ggctccgtgg gtacgggtga gtggattgtc ctgtttgtgg tcgtcctgat tgtggtcggc
     1981 cccaaacggc ttcccgagat cgcccgcaaa ttgggccgta cgatggagat gttccgtcgt
     2041 gcggccgacg aatttaaaga tcagttgctg aacatggacc gtgaaccggc ggaggcgacg
     2101 ccaccgcctg aaacggacgc tgcctatgac ccggacgggt acggggtgcc gtccgacccg
     2161 gcggaaccgt atgacgggac accgtatccg gacacgggcg agtatgccga tgcaacggat
     2221 gtgccggggg aggcgacacc ggtttcggac gggattccgg aaacggacgg gagcgccggc
     2281 gacgcggcgg cggaaccggc ggcttcgtct aaaccggaac agcccacgga ggacgtctca
     2341 tgaaggtcgg tcgtttctcg ctgcccaaat tcggacaggg ggatgagtat aatgacccgc
     2401 cgcgtccctt tctggagcat ctgattgatc tgcgcgattg tctgatccat tgcgccatcg
     2461 cttgggttct ctgtgagatc ctgattgtcc ctttcgcgcc ccggatcacg aactggctga
     2521 tcgcgccggc cggagtttcg aaagacatgg tgcaggggct aggctggacc gccggtttca
     2581 gcatcctgct gaaaatcatg ttgtggggtg gcacggccct tagcctgccc ttcctgctgt
     2641 tcttcatcat gcggttcgtc ttccccggac tcaaacgcag cgaacgttct atcatcatct
     2701 tttgcctttt cacctcctcg ctgctgttcg cgataggcgt gtggatggcc tatgccgcca
     2761 cgctggagat cgccatcggt gtgttgcaga agatcaatgc gtgggtcgga atcaaagtcg
     2821 atatcgtgcg cctcgacgag catgtggata tcgtgatcaa gaccatcatc gcgttcgggc
     2881 tggcttttca gttgcctttg ctgttgttgg tgctcggctg gatcggactg gtcccgtccg
     2941 aagcactccg ttcgcgacgc cgcatcgcca tcgtggtgat tttcgtgatc gcgatgatct
     3001 tgaccccgcc cgatccggtc tcgcagatct tgatggcggt gccgatgtgt gcgctgtacg
     3061 aggcctgcat ctggatcatc cgcctgcgtg aattggcgcg gggggacgag aaagacgatg
     3121 aaccctccgc gccggacaac gacgcggact gagaattcgg gaggcacgcc atggcacgta
     3181 cggcggcacg cacacgcacc acacgcgaaa ccgatatcgc ggtgacgctc aatctggacg
     3241 gcagcggcgt cagccgcatt gaaaccggtg tgggattttt cgaccacatg ctggaactcc
     3301 tcgcgcggca cgcgctcgtg gatcttgagg tccgcgccaa gggtgatgtg catgtggatt
     3361 accaccatac cgtggaggat gtggggctgg tgctcggcgc ctgtctcaat gaggcgctcg
     3421 gcgagcgtcg cggcatccgt cgttacgggt tcgcgctgtt gccgatggac gaggcgcttt
     3481 gcgaggtggt gatcgatctg ggcggacgac cgtttctggt ctttgccagc ccgatgaagc
     3541 acatgcgggt gcgtgatttc gagatgcaac tgctggagga gttcttccgc gccgtgacgg
     3601 tggagggacg cctgaacctg catgtgcggc aggtgtacgg cgatgagacg caccatgtgt
     3661 gcgaggcgat cttcaaaggg ttcgcgcgcg cgttgcgtat ggcggtcgag ccggatccgc
     3721 gcgaaaccgg tattccgtcc agcaagggga cgatcgcgtg atcattctgc cggccatcga
     3781 tttgaaagac gggaagtgcg tgcggttgcg ccaggggcgg gctgaggagg tgacggttta
     3841 ctccgacgat ccggtggcgc aggcgcggga gtggcttgcg caaggcgccg aacagttgca
     3901 tgtggtggat ctggacggcg cgtttcaggg cgagccccag cacatggaac agatcgcgca
     3961 gattgtccgc gccgtcggca ttccggttga ggtgggcggt ggtctgcgca cggatgcgca
     4021 gattgaacaa atgttggggg tcggggtcgc gcgcgcgatt ctcggcacgc gcgccgtgga
     4081 gagcctggac acactgcgcg cgctggcccg gcggttcggc gcggccatcg cggtggggat
     4141 tgacgcccgt gacggatttg tgcaggtccg gggatgggtg gagaccacga aggtccgtgc
     4201 ggtcgatctg gcgcggcggg tggaggatgc aggggtccgt acgatcatct ataccgacac
     4261 cgcgacggac ggcatgttgg gcggacccaa tctcacggcc ctgcgcgaaa tgtgtgcaac
     4321 ggtcgcgtgc cgtgtcatcg catcaggcgg tgtgagcgcg ccggaacacg ttgccgcact
     4381 ccgcgatctg ggattcccga acctttacgg ggcaatcgtg ggcaaggcgt tatatgacgg
     4441 caaaaccaca ctcgcagcaa tgcgggcagc ggcgtgctga aggggattat gctcggaaaa
     4501 acaaagatca cccgactgaa gaacggggtg tcggtggtca cgtccgccat tgaaaatgcg
     4561 gaaagtatcg caatgggcat ttggatcggc gtcggcgggc gccacgagcg ccgtcccttg
     4621 gccggcgtga gccattttct ggagcacatg ctgttcaaag gcacgccgac gcgttcggcg
     4681 ctggccgttt cgcaggcgat cgagggccgc ggcggctacc tgaacgcgtt cacgcaagag
     4741 gaggccacct gttactacgc gcgcctgccc tatgaatatc tgccgcaggc gttcgatgtg
     4801 ctgtcggaca tgtacctcca cgccgcgatc cgcgaggagg atttccggcg cgagagggat
     4861 gtgattttgg aggagatcaa aatgtatcag gatctcccgc agcatgtggt gcaagagaac
     4921 ttgctggagg cgatgtttcg cgatcatgcg ctcggagcgc cgctttccgg caatccgaaa
     4981 tcgttgggtg cgatgagccg cgagaccctg ctgcgttaca aggcacgggc ctacacgccg
     5041 ggttcgaccg tgttcgcctt tgcgggcagg ctcgaccatg aggcgtgtgt ggcacgggtc
     5101 gaacgggctg tcggcgggca gccgcgtcgg cgcgggttgg atttcatgcg tgtggacggc
     5161 agggtgcccc aaaggcggct ttcgctgacg cgcaaggaga tcgcccagac gcacgccgtg
     5221 atcggattcc gtattttcgg acgtcatgat gaccggcgtt acgccttgcg ggtgttgaac
     5281 ggtgtgctgg gcgaaaacat gagttcccgg ctgttccaga gtgtgcggga gcggcgcggc
     5341 ctctgttact ccatcaactc aggattccaa ctgtttgaag agaccggtct gtttaccgtc
     5401 agcggcggtt ttgacgcgcg gcgcacgatt gcggcgctga aacggacggc gcaagagatg
     5461 cggcgcctga tcgatgcgcc ggtcggcgcg cgcgaattgc gccgcgtgaa ggagtatctg
     5521 cagggcacct tccggctcgg attggagggt gtcgggaacc agatgatgtt tatcggcgaa
     5581 tcgttgttga attacggccg ggtcgtctgt ccggaggaaa cgctcgcggg gatcgcggcg
     5641 gttaccgcag acgatgtgca acgcgttgcg gaagaggtct ttgagccgtc gcgcctcacg
     5701 ttgtcgctgg tggtgccgaa cgcacaggcc gaaagcgaag atgagtggat gtcgagtctg
     5761 ggcgcgttgt ttgcctgacg gacggactgc ggaaaggggg cgcgtatgcg caaagcgggt
     5821 cagggttcgg aaacgcctct gcgcgtaccg ccggacgaga actggcgtac cacggatcag
     5881 gatgagatca accgccggaa acggcgtgcc gccagcgagc cgatgaccat cctgaatcgt
     5941 acgccggctc atcccgtgtt ctcgaatttc aacgtcacct cccggagcag cggtcagacc
     6001 tatcaggtcg aagtgcggga gatttcgccg cgagtcggtt cctgcacctg tccggatttc
     6061 cgggtcaacg acctcggcac ctgtaaacat ctggaaaggg taatacgcga gctggcgtgc
     6121 ggcgagcccc tgtccgaccg catcgatgtg gtgccggacc gggtcatggg gcgtctgctg
     6181 atcgaacgga acttgaaccg gctgccagcc gccctgcgcc cttatttcga tatcaccggc
     6241 ctggcgttgc ccgagttctc cccaacggag attctggagg catgcgaacg ggtcggtctg
     6301 gacgtggtgc gcgtttcgca ggatgtcgag ccctggatgg cacgtctggc acggcagacc
     6361 gaacgcatcc ggttgcgccg ggcttacgag catgcggtgt gttccggaca ctacccgccg
     6421 caagagactc tcgaaccgct cttgccctat cagcgcgagg gtatgctgca tctggccttc
     6481 acgggccgcg ccctgctggc ggatgagatg gggttgggta aggcggttca ggcggtcgca
     6541 gccgtggcgt tgttgtaccg gctgggcgag gtgcgccatg cggtggcggt cgtgccggag
     6601 gctcatcagc gggagtggga ggggcatatc cggcggtaca cggcgctttc gtgcgaaacg
     6661 gttgcggggg atcgtgcggc gcggcggcgt gcgtatgagc aaaaggccgc gttcacgctg
     6721 gtcagctatg agcaggtgtt gcgtgacagc cgtctctttc tggacgtgct gaaccccgag
     6781 tgcgtgattc tcgatgaggc gcagcggatc cggaattgga acacgcggac agcgcagacc
     6841 ctgaagcaga tcgagagccg gtaccttttc gtgttgaccg gtgcgccgct ggaacagcgt
     6901 ctcgacgaac tctacacact gatgtcgctc atcgatccgg aggtgctcgg gccgctcttc
     6961 cggttcaacc gcgagtttta cacgctggat gaggacgggc gtcccacggg ttaccagaat
     7021 ctgcaccggt tgcgggaacg tatccggccg tacgttttgc agcgtcgccg caccgaggtg
     7081 gagactgaac ttcccgaccg tacggaacgg gttgtgtacg ttccgatggc gccggaacaa
     7141 cgcgaccggt acgcgacgca tgaaacccag gccacgcttc tggccgcgca gtcgcaacag
     7201 cgtgcgctga cccggtcgga gaccgagcgg ttacggcgtg agatcgccat gatgcgcatg
     7261 ctttgcgata cgccgtacat tacggatgag acgagccgtg tctgtcccaa actggaggaa
     7321 ctccgcaacg cgatggagag tgtcctggcc atgtccggag tcaaggtttt ggtctgttcc
     7381 gagtgggagc gcatgttgca actggtgcgc gcgttgtgcg aacagcaggg gtggggctat
     7441 gcgtggcatg tcggttccgt gcctcagggg cagcggaaac gcgaaatccg gcgcttccgc
     7501 aacgagcccg cctgccgtgt ctttctggcc acggatgcgg gctgtccggc gggaagccta
     7561 tcgcaggcga gtgtggtgat ccaatgtgat gtgccgtgga ccgcggcgcg gtttgaacaa
     7621 cgaatcgcgc gggtgtgtca ttcgcatcag gtgcgcagtg tcacggttct gaatctggtg
     7681 gcagccgaca gcatcgaagc gcgtttgctg gagcagctga agcagggacg cgccaacggt
     7741 gctgcggtcg gcgcgcgtgt ctccttgacg cagcgtctgg cgacgttact gcctttgacg
     7801 gaggcgacgg agcggacacc tgtggagcat ccgtcctttg aggcgttgcg ggcgctcgat
     7861 ccggaattgg ggtttgcggc cgcactcgcg gaacggatgg gtgagaaact ggtggcctgt
     7921 gacgtgtggc acacggaaga gggacgcccg gttttcctgt tggccgccga aggaccgctc
     7981 cgtgtggcca atctgatcgc tgatcggttg gctgccgact ggttcgcgca tgtggcggca
     8041 ggaccggttc ttgaggtggt ggcgcccggt gtcagcgacg tcctgcggcg gttcatggat
     8101 aaaggatggt tgcggatgcc gcatcccgtc ggcgcgcgca ctctctatca acgcgggcag
     8161 gggctctccc gacccagaat gatgccggat gacgagcagg cccggattct ggagttgcgc
     8221 gagcaggcgg gacaggcgtt tcgtcaagca cggcggctga tgaggcagct ggcgttcgat
     8281 gcggcacggg cgccgctgga gttgtccatg gtactgctgg cacgagcctt tgcccggcag
     8341 caccgtcttg ccgaaccgcg gcatgcgcgg cagacgctca ccgaaccgta cgcggagttg
     8401 tggggcgacc tgctgccagt tctgcgcgag gggcttcagg aaaactcgcc ggtggcattg
     8461 tcccgcgcgt tgatggcgaa attcggcagg taacaaaccg aaggcgccga ccggagcgcg
     8521 tggccacgga tcggatcagc cgcgtatttc cgccaccgta gcgcctaagg cacggagttg
     8581 cgtcacgaaa tccgggaacg attcgttcag aatctcggca tgccgcacgc ttaccggttc
     8641 aggcgcggcg aggcccgcca gcgccagaga catggccaga cggtgatcgc cgtgggcttc
     8701 acagacgcca ccccgcaggg atccgccatg gatcatgaac ccgtcgcggc gctcctcgac
     8761 ccgtgcgccg agcgcgcgca gttccgcgca cagcaccgca atccggtccg tctctttgta
     8821 gcgcagttct tcggcatcac gcacctcggt gacgccgcgc gcgaaacagg cggcgatcgc
     8881 gaagaccgga aactcgtcga tcatccgcac gacacgctca ccctccaccc gcacggcttg
     8941 gagcggcgcg gcggtcaggc gcaaatcgcc cacgggttcg cctgccgcag tctgcgggtt
     9001 ctccacggtc aggtcggctc ccatttcgcg taacgcatcc agaatgccgg tgcgcgtggg
     9061 gttgaggccc acgtcgcgga gcaggatggt cgagccgggg gtcagggcgg ccgcgaccag
     9121 caagaacgcg gcggaagaga tgtcgcccgg cagggtgagg gcgagcgggg ccaggggccg
     9181 ggcgaggggc gtgacccgca cttcaggacc gcaaacgcgg atgtccgcgc ccatggaggc
     9241 aaggagacgt tccgtgtggt cgcgggaggg accgggttcg cggagaaccg tttcgccgtc
     9301 cgcagcgagg gccgccagaa tcagacagga cttgacctgt gcgctcgcca cgggcagggt
     9361 atacgcttgg gcgcggagcg gcacgtcggg gctacgcgcg gcaagggtga tgggtgcgca
     9421 gtggttggga ggagtcgaga tgcgaacgcc catggcgcgg agcggttccg tgatgcggtc
     9481 catggggcgc ttgcgtaacc cctccgatcc gtcgagcaca cagggcgtgc ctgccgccgc
     9541 gagcgcgcca gtcagcatac ggatcgtggt ggccgagttg ccgcagtcaa gcggggcggt
     9601 tggcggtgtg aagccgtgga gacctacgcc gggaacggtc agccggtttt cctccagacg
     9661 ccacgccacg cccaaggcgg tgagtgcatt gagcatcgca cgcgtgacgc cggagacgag
     9721 gaatcgatcc accacgctga ccccttcggc cagggcagcg aagagtgctg cgcggtgcga
     9781 gagggatttg tcgccgggaa gaaccgcttc accggtcaag ggccggtctg cggaaaagga
     9841 aagtgtgagt gagtgttgca tcgcggatga taatccttta gggtgtgggg taaaggttgg
     9901 tgggccgttc ggccacgagg gtgcgcgttg cggggtgcgc gaggcgccgg ttgtactcca
     9961 gcaacgccgg gtaaagttcc gcagggagtt ccgcgctctg gcgcaccagc gcacgtgtga
    10021 tcgaaatctg tacacgtccg tcggcgcggg ttatgtgctt cacgctgtag tccagtgtgc
    10081 cgagaccgtt cggcaggatc cagtgcttag attcgggaag cacgggcagg cgcgtgtagc
    10141 ccggcggtaa aaggatttcg caagtcaggt cagatctgtc acgcatcccg agaaacagcg
    10201 ggttcacacg tgaatcggcg cgcaggggga agagaacccc cgccacctcg ggaatgagaa
    10261 gcgtcagtgt gcggccttcc agcgtggcgt accgcgcagc agtgaccgta taggtccgca
    10321 aacaggggta gccttcggtt tcggtgatca gcgcggaggc gggttccgct gcccgggcga
    10381 tcttgctgac gagttcgagg tgatggcggc gccgctcttc tggcagcatc tcgcgatact
    10441 gcttgcggaa ggtgccggcc tgagttccga aaaaccggtt gctgaccgtg atccgtgcgg
    10501 caccggatac atcgagatcg atcgtccacg catggtgcga acggttgcgt aacggttcgt
    10561 cgagttcgat cgtcacgggg cgtcctttga gcgtgagtgc cggcgcacca tcgagttctg
    10621 aactgccaag ttcgctgtac tgatcccctt cattgagata acagatcccg tcccggagtt
    10681 tcactgccac cagtggatgg cggtaaaact cgatttgggg gatctcccgc attggctgcg
    10741 aaaaggccgg atagcacgtt gtgtcttggc cggcgaagag caattcggcg tcgaatccca
    10801 cggcgcgcag catggcccgc agcagcaagg cgcggtcggc aggatggccg tattggtcgg
    10861 cgagcgtgcg gtcgggcggg gagaggcact ccgctggcag gtcgaaaaac gcggggcctg
    10921 cgggtcggat ggtacgcaac acctcgtcgc ggatcgcgag cagacgctcc cgcggatcgc
    10981 ggaggccccg gacccgctct ttcgcacggc ggcgcgcctc gcgatccgtt tcggcagccg
    11041 tctctgcagc gcgtcgcaac tgccgcatat actcggacca gtcgccgaag gagacaaaga
    11101 ggacgggacg gaaaaaatgc cagggcggga gcagttcctc cgtgcgggtg accggttgct
    11161 gcggggcgtg ccaggtatgg gtgaccagac cgtcccgggt ttccgaatcc atggtgacgg
    11221 aaccggggtt gaccaaccgg atggcggggc tgagcgtgtc gggataggtg agccggtaga
    11281 ccctctctgc aactggggcc gttccgccga aagtgtgcgt atgacagtaa aagggggcgt
    11341 tggtctgtgt gaaacgagtg ctcaccgtga tcacgctgcc ggtttccaca cccggcagat
    11401 tcaccacgag cagtttgcct gcggggtatc gtggtgccga tccggtccag gccgcgtcca
    11461 tctgattgat ctctttgggc gtcacggtgt gcacgatccc gttagcgttg gaaaccacgg
    11521 cggcaagcag gtcaatctgt tgccagacgg gattgaaggg gattttcagt tccgaacagc
    11581 tcttcttgcc cgcataggtg aggattcgtc gggaccaagt gtgggtggtg gtccaagcgt
    11641 gcggcgaaac cagatgcgct tcggtgagat cgtggagtgt ctcgtggtcg ggcgggttcg
    11701 ggtcacccgc ctcgaacagc ggtcgtttcc gtcccgcgaa ctcgatttcc ttgaggatgc
    11761 ttttcaattc gagataatcg gatggagaga attccggcgt tttcaggtga taggtgagtg
    11821 acccggaaag cacggagttg ctgacggatt ggctcatggc aaagaaaacc cccgcgcggt
    11881 cgatatgcac cgcgccgggc agggtgtggg gcgcacccaa ccctgcggcg gcatgcagcg
    11941 cgatccgttc ttccgtgccg caggtgatgc cggtttccag cggataccgg cgtttgtcca
    12001 gtccggtctg tccgatgacg aagttcgcat aacctaatcc ggtgccgagc cagggcatgg
    12061 cgagcaggtc caacccttca ccgcgcacag ggaactccgg aacgcgggtg gtcaaggtca
    12121 ccgcgagcgg ggtttccgtg tcgcgcaaat cctccggggc gatcatacat tcgatcacct
    12181 cggcaccggg aagccggttc ttgagagccg cttcgaaaaa gttgcgccgc tgttccggct
    12241 tctgccggac gaggtaactg cggtaggcgt tgtcattgat gccctcaaac cggatgcgcg
    12301 tcttcagtaa caacgagccg ttacgatcca tcgtgccttc cgtgttaatg cgggtcagat
    12361 tcttctctac ggggtacacg tctgagactc gtagggtttc accctcaggc cgcgccacca
    12421 gatagctgcg gttacagaga taggccggaa aaaggtcgcg ggtgttctcg tccgtggggt
    12481 ccatcaacag gtagccgccg tccgggttat ccacagcggt gacggcgtga ttgaagaacg
    12541 gcatgggcac gtcggggtcc atcttggcgc ccgcgtggat cagcacgggg taggcatcga
    12601 tcccggccat gcgcaacagg gcaaccagca aagcggcctt gtcacggcag accccgtagc
    12661 ggttgtcgaa ggtcagactg acgggatgcg gttcatagcc gggggcggtg tcttcagtcg
    12721 tgatgcccat gtagcggatc tcctgtgaaa cgaatttgaa gatccgccgg atggtctcgt
    12781 cgcgcgaggc ggaacctgcg gtcagggcat cgacccgggt tcgcatggcc gtgtttgtgg
    12841 cggcgagggc gggtgcgcat aactcccaat accagcgcga aacggtccgc caatccggga
    12901 tcgtgctcag caacaaacgc tgcacctggg tgtgcaaggg cggcatatca ggttcgggat
    12961 acatctgcgg cacgttcgtc acggtccaga ggtgaagggt ccggccttca gcttcgcggc
    13021 gctcggtttt catgaccgtg cccggtactg gggcgcgtaa cagcgcatga cggaacggca
    13081 gttccggcgg ggccgagatg gcgtactcca gccggacgat cggcgagtcg tattcaaaca
    13141 ccgcgtagtc ggcccaggtg tcgggcatgc gcaccttgac ggttttgcgg caggtgatca
    13201 gatggcatac gtcgccgatt tcgagacccg gcagtgagag gttgaggatc ttattgttcg
    13261 gatcgtaaat gttggcaccc atctgttcgg attcgaccac cacgcgcgcg tacgatgcga
    13321 tgtcgagtgc aacggttcgg ccgtccggtt tgatgacttc agcgcgatac accagcgcgg
    13381 taccgtaatg cgcattgaac gggatgcgga cggacgaggc gtcacgccga cccttttcag
    13441 tcagaatctt gttgtactcg tcatcccaga ccaccgaggt gccgtcgcgc tcgtaccgtt
    13501 cgagcacgcg gttatccacg agtacgcggt cagcgttcgg aaaacgttcg gcggtgaccg
    13561 aggcggctac ggtcatcacg ctttggcggt caagcgggaa cgcctccggc aggacttcgg
    13621 cacccgatac gtacgggaaa aacggaaaca gggcaaacag aaaaaaagaa cggttcatgg
    13681 gcactccttg tccacccctt tgacgggtgg gacacctttt cgtggcgtcg gtgtcggttt
    13741 cgggatcctc ccgatttgac aggcgtatcc gaaacaggac tagtaaacca aaaacagcgg
    13801 ttccgctcca gtctgctcat gcgtcaggcg gcccccgatc tggttgatcc cgaaagcgat
    13861 cccgaccccg accccgattt ggatgaaacc ctgaaacctt gaatccccga aaccaaagag
    13921 agttgaaggt tcaactgctc tttttaggct aaaaaacagc cgtaccagac ctgttgagtc
    13981 cctcaaggca tacggaggcg gcccccagca gggctgcctc gtatcccaag cttgtcggta
    14041 tgacgcgcag tgactgacat tgc
//
LOCUS       DAOSLE010000070        13881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4499_length_13881_cov_6.327137, whole genome shotgun sequence.
ACCESSION   DAOSLE010000070 DAOSLE010000000
VERSION     DAOSLE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13881
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_13881_cov_6.327137"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PK576_06220"
     CDS             <1..659
                     /locus_tag="PK576_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61225.1"
                     /translation="LYHRSTHPQPRVTKRLFGCVNIWNTYDKRGETYFLEQEAFRAAQ
                     AATRFLFVIGWSFRAPDVVLKHKRLIRTIFTPVTRHRDAVARLSGALRAHADHVVGVH
                     IRQTDYKKFSGGRYFYSLDTYKGFMRQMEEQLPGRTRFLLCSDIRLDPTEFRDFDILT
                     GTGHPVEDNYALATCDYLIGPPSTYTAWASFYGGAPKCFLRSTDMRITPECFVIQHSV
                     "
     gene            932..1963
                     /locus_tag="PK576_06225"
     CDS             932..1963
                     /locus_tag="PK576_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61226.1"
                     /translation="MLALTATLFPLEYVPRLYFLFAVSAVLLVCCFLSFIPWLPPQLK
                     IAFPLTVVLTPHYGEVFFTLTNIHWIVALLFPFLALGVPIRSTSHLTRLSILIVVLAL
                     SDPFILFFYPLFFIRRLAHRGSERGEGVFFLVATFCACLQAGVLLAAPRGAAKTFDTD
                     LWHWLEALLLRPVAGLFLGKEVALLGYTSTPVFILIGLALGLFLCTVLYSIITTTPQN
                     RRILLFFFSVSAIAFTAAFLRSYDRPLVFAPFYGADRYYYIPYAFMIFGVLWFTHTVS
                     GWRKRLGFAACFLILMSSVSVFQLPRRLDSHWTDHIKQLREQGHVRIPINPPGWSVDL
                     YETPTSPDD"
     gene            1981..2874
                     /locus_tag="PK576_06230"
     CDS             1981..2874
                     /locus_tag="PK576_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE61227.1"
                     /translation="MKKHHILSFIDKTGYGLEIGPSYNPLAPKRLGYNVRTLDYLSQS
                     DLIEKYSRLSVPVAEIEPVDFVWSGEPYSELMGGHSLFDWVIASHVIEHMPDLIGFLK
                     NCTSVLKEDGILAIVVPDKRYCFDHFRPPSSLAQIIDAHQAKLTHPSAGMIAESYLNK
                     AYRNGEGIWNLLTRGPLRFQHTVSETKNAMALREYTDVHAWSFTPHSFRLAIHDLVSL
                     GHLDLKEVSFRITSGFEFLAVFRKGKSPPSSSRLCLAKRAHSELSPSLLLAILERLGV
                     LFYMPLFFVKRWLSPKDRRQG"
     gene            complement(2922..3431)
                     /locus_tag="PK576_06235"
     CDS             complement(2922..3431)
                     /locus_tag="PK576_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE61228.1"
                     /translation="MGNEFKLNLGCGEKVKSGYVNVDACGQPDFLYDLTVFPWPWEDA
                     TVDEVCMEHFLEHVPDYDRTVREIHRILKTGGVLRFTVPHFRNPMAAWHLHCNQFSVY
                     TPELLCEQRAYQWGGRVLFEKISLRIRFVWITPWLGRILSFFANRAPLKWDWLGLPID
                     EIEFVGRKK"
     gene            3549..4289
                     /locus_tag="PK576_06240"
     CDS             3549..4289
                     /locus_tag="PK576_06240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE61229.1"
                     /translation="MLISLVLLTWNRYRFLDLCLASLFANLSGAFDYQILLLDNGSTD
                     LTPQVLNRYRNKPGVTLLANAENRGLSAYKTLFNKAKGKIIIEVDDDILDFPKDFDQI
                     MVDYLAAYPDYGFLALNVIQDERTNGAKPDPSHYAEDTRGNKTVEQGPAGGWCAGFRR
                     KDYRAIWLPFNLIPLSMKRCEDGTLTALFRRLRGLKAGVIREAVCLHACGPYYAQQYG
                     QTEREIQKYMAAGLTELADSYIQTQKGV"
     gene            4419..6791
                     /locus_tag="PK576_06245"
     CDS             4419..6791
                     /locus_tag="PK576_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61230.1"
                     /translation="MARLTVGLHLTPHPTGGISCTWEDDSGASHTLETCSTETGQVSF
                     PIPIQAKLFRFHFFPSHEPYRINGLSLFHLRLLSAQWLNNMLTPEHPVYTQQYMGKRD
                     CLEIIATEGTSLTYPRFFSTLLHFARRLRTVTPVLLFGLFCLPLGLKLRFCGVHRPFS
                     LPRGLCLPHEEAPLRKGDRSVFIFFVAALLLCLPNPVAPTTPGCDASWSWLLNHFALN
                     PSGIGTSFFFTYGPLGFTITPEAIGNNAFWALAFSLLYILLFSATIHIILTQHETPSA
                     CACPLFLCILLLPPNTEWRWAFLINLMLAACLFFPPEQTRARAVYSAVTGAVILYASF
                     IKFSLAITAGLNVLMVLLLLPPCQRRDLWFFGVPLAGGFLVGLLFARWFLFSSFSSIL
                     TWARLSWEIASGYNTAMVTQVTWLELIIPAILVASFLVLLFPLHSFSLQRFKRMALVS
                     TTLFFAFKYSMTRAGIGTLRAVTYVVPCMTGILMLFADSLWRPNLARLFRFQLAVCVG
                     LSVLTGVFLQLPIIGFNYKNLLDSTRLNQCIAHARTRSDQALLDVRLPPHWIRKIGNN
                     TFSSYPTELTYAPANSLRFIPFPITQGYSAYTQDLDVHGASLFSGPQAPEWLLCAFSK
                     PDQRNGVVDTPAVWNAVRTHYRPEDVNEHGVLLQHRPHTAIPQWRCVRQQTAAVGEWI
                     DLTGKDMTNTCLSIKWSPTLLGKGLALLFRNTQCSFTLIRVDGTTHRWRLVPDTASTP
                     FPIGAIPFTDREFPRIFDNPDSAPFPVSRIKFECEHPWYYSRQITLSFFQ"
     gene            6913..7770
                     /locus_tag="PK576_06250"
     CDS             6913..7770
                     /locus_tag="PK576_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE61231.1"
                     /translation="MEADLEVYLTERRRLVEEHLSACLPEATPRNKRLIEAMCHAVLS
                     GGKRLRPILCLAAAEASGGSLRDALCPASAVELLHTYTLVHDDLPCMDNDPMRRGQPT
                     VHAKFGEALGVLTGDALQTLAFEILARTPEKQAGTLQRLVTELASAAGQAGVIAGQAE
                     DIRCAAAPTRADIEYVFRHKTADLFRTACRLGAIAANAAAPDLERLTEYGDGLGFAFQ
                     ILDDLLDAHEAKTRAEPELSCLAVMTVAEAREWAENLTQQAVAALAGLPGETAPLEAL
                     ARHLLGRLA"
     gene            complement(7818..9419)
                     /locus_tag="PK576_06255"
     CDS             complement(7818..9419)
                     /locus_tag="PK576_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61232.1"
                     /translation="MRIRCAVTIRMILWGAAMTVGIAAAATRPVGADCVPDDLLDDAE
                     AINKALAAAQPGDTVTLPAGVLRLFSPLTPPSGVTLQGAGQERTILAFGGEKPHEFVR
                     MRDVSGVQVCHLTLDGGSNPLATQGVVAMKCDTLCLHHLTIRDFVDTGTFGPHGILLS
                     QTSDSVIRDNTICNIAPEDPWGAGIRVSDRSLRAVIERNVIDRTGRGGIFTNNGASDA
                     VIRENVVTRSGGIGFAIEVHSESVRTLVEDNVVDHGLSIVSSHCAVRRNLVADAGGTW
                     KAYGIEGGGGPYGIVTDNVIHYGQIEGISVSGASHHMLWAHNRFVCCSMWGMQLQGPS
                     ASNHIHSLYFYDNLFTRTQKGHPSVVYKGADGHAIRINNHTTHVTFDGNQITDNGGMA
                     FQILGKNVNHLTFIRNVIANNAGASIRTYLGDAVVWEDNLVSGNKTNVTLTTCGEMGA
                     APVAAFDAPETARAGEPVAFRDTSVDPEGIAQRLWDFGDGLPCLEAQPKHVYRKPGAY
                     RVTLVVWNASKRAALAERIVRVYGE"
     gene            complement(9438..10595)
                     /gene="obgE"
                     /locus_tag="PK576_06260"
     CDS             complement(9438..10595)
                     /gene="obgE"
                     /locus_tag="PK576_06260"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE61233.1"
                     /translation="MKTRKIVDRVVVHVRAGKGGDGSASFRREACVPRGGPDGGDGGR
                     GGHVLFRGSRDVDSLIGLSFAPQLFAENGVAGRGQKMYGRRGRDRVVRVPCGTSVFDA
                     ETGELIADVVGHDEEVVIARGGKGGLGNVHFKTATHQVPTEHTPGEPGEEFRLRLELK
                     TIADAGLLGFPNAGKSSLLACLSGARPKIANYPFTTLNPIVGTLMYPDFSQLRVADVP
                     GIIEGAAAGVGLGTSFLKHIARSRVLIFVVDMAGTDNRLPWKDYLALRNEIAQHNAEM
                     LDRPSLVLANKMDVAAAAEYLPRFVRETGVTPIRVSAVTPDDPGVAEVKQSLWDLLRP
                     APRGVWTAVGTAEPETGTPSDGDGEAGIPEVSLRRAPFLDLSKKTPKKKRH"
     gene            complement(10625..10870)
                     /gene="rpmA"
                     /locus_tag="PK576_06265"
     CDS             complement(10625..10870)
                     /gene="rpmA"
                     /locus_tag="PK576_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE61234.1"
                     /translation="MAGAASRNGRDSNAKRLGVKRYDGQVVRAGSILVRQRGTRFHPG
                     KHVGCGRDYTLFALKDGTVKFQKGGAVVSIIPAVAAE"
     gene            complement(10888..11202)
                     /gene="rplU"
                     /locus_tag="PK576_06270"
     CDS             complement(10888..11202)
                     /gene="rplU"
                     /locus_tag="PK576_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOE61235.1"
                     /translation="MDAYAVLETGGKQYRVAVGNTIEIERLDAEAGQDVVLETVLALS
                     DGQELKVGMPYVPDAKVTLSVIGHKRGKKLINFKKKRRKGYSRRMGHRQELTVATVKA
                     IA"
     gene            complement(11269..11682)
                     /locus_tag="PK576_06275"
     CDS             complement(11269..11682)
                     /locus_tag="PK576_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE61236.1"
                     /translation="MARILIVDDEPGLLMFFQRLLNTNGYDVVVAEDGVTALDVIRKT
                     EIDLIISDLRMPRMDGMTLLREVKAIKPDIPVIILTAYASDETAIESKRIGVFEYRSK
                     PFNAEKLLDTIENALGAAKNKEAGPLDSRGGDPVK"
     gene            12145..13710
                     /locus_tag="PK576_06280"
     CDS             12145..13710
                     /locus_tag="PK576_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61237.1"
                     /translation="MSCATGVVLAAFCWARADGPFRVLPYLQNPATDAITVMWFSETS
                     TTGQVAFCQTGVWSAPLEVPAVLASALDYHSSETNSPPPLPHPPLPYLHRVRLTGLLA
                     GTAYEYVVTQDEVSITNSFRTAPPRDSAVRFVVYSDSETEPESTGSREKWPEPNGNAD
                     RLYPVDQTRGYQENLQIMAARNPDFIVIAGDLVEAGGEQRDWDEFWRHNAGVLGDLAS
                     TVPIMPALGNHEYYGGTRGGYSQPTSEVSVAKYLTYFELPANGAANPAHNGRYYRLDY
                     GPVTLVVLDLCNGDDTDPAKDTSTYLKTADGCQAPDFNPGSPQYAWLEEQLASAQTNS
                     PFTFVAFHQTPYSVGPHGKADESQSGVPVRVLTPLFLKYGVDAVLAGHDEMYERSVVT
                     GIEQLPGGGTRPVTLSFYDIGIGGDRLREPFSGLVNDKQVFLAHDDAPEMWSGARLVS
                     GGKHYGHLEVNVRVNGQGKWEAVLTPVYAFPLMDSGGTVTGFERRTYADEVTIISASA
                     PLLGQKGAIVTEW"
BASE COUNT         2723 a         4415 c         3591 g         3152 t
ORIGIN      
        1 tcctctacca ccgttcgaca cacccccagc cccgcgtcac gaaacgcctg ttcggctgcg
       61 tcaacatttg gaacacctac gataaacggg gcgaaaccta ttttcttgaa caggaagcgt
      121 tccgcgccgc ccaagccgca acgcgcttct tgttcgttat cgggtggtct ttccgggctc
      181 cggatgtcgt gctgaagcac aagcgcctga tccggacaat attcaccccc gtcacccgcc
      241 accgcgacgc ggtcgcgcgc ctgtccggag cgttgcgagc ccatgccgat catgtcgtgg
      301 gtgtgcatat ccgccagacg gactacaaaa agttctccgg cggcagatac ttctactctc
      361 tggacaccta caaagggttc atgcggcaga tggaggaaca actgccgggc cgaacccgct
      421 tcctcctctg ttccgacatc cggctggacc cgaccgagtt ccgggatttc gacatcctga
      481 ccggcaccgg gcacccggtt gaggacaact acgccctcgc gacctgcgat tatctaatcg
      541 gcccccccag cacctacacc gcctgggcct cgttctacgg cggggcaccg aaatgtttcc
      601 tccgatcaac ggatatgcgt atcacaccgg aatgctttgt cattcaacat tcggtctaac
      661 ctctcttttc gctatgcaaa catccagtaa tgcgtctcgt cttagtcctt ggctacctct
      721 caatctgccc atccaggcgg atatcacatg gtcgtcgcgt gtcatcctcc tgctcggaat
      781 cacagccctc ctttttttcc gggcacccca tgagttcgtc tcgcctcaac tttggggaga
      841 ggacggaccg attttcctcg cccaagcgcg aacacacggg atcgcttcga ttgccatacc
      901 gcacgcgggg tatttacatg tgttgccgcg catgttggct ctgacagcaa cccttttccc
      961 ccttgagtat gtgccgcgct tatatttcct ttttgcggtc tccgccgtgc tattggtttg
     1021 ttgttttctc tccttcattc catggctccc gccgcaattg aaaatcgcgt tccccctcac
     1081 ggtggtgctc acaccgcatt acggggaggt tttcttcacg ctaacaaaca tccattggat
     1141 cgtcgcactg ttgttcccct ttcttgccct tggagtaccc atccgttcaa cgtctcattt
     1201 gacacggttg tccatcctga tcgttgtgct tgctctaagt gatcctttta ttcttttttt
     1261 ctaccccctt ttttttatcc gcaggctagc acaccgaggc tccgaacgcg gagaaggagt
     1321 tttctttctt gtcgccacct tctgcgcttg cctgcaggcc ggtgttctcc ttgctgcccc
     1381 gcgcggagcc gcgaaaacat tcgacaccga tctctggcat tggctcgaag cccttttgct
     1441 tcgtccggtt gcggggcttt ttcttgggaa ggaggtcgcg ctactgggat acacctccac
     1501 cccggtgttt atcctcatcg gcctcgcgct gggactcttc ctctgcaccg tactatactc
     1561 catcataacg accacaccac aaaaccgccg gatccttttg tttttctttt cagtatcggc
     1621 catcgcattc accgcagcat ttttgagatc gtatgatcgt cctctggtct tcgctccctt
     1681 ttatggggca gaccgttatt attatattcc ttatgctttc atgatttttg gggtactctg
     1741 gttcacccat acagtatccg gatggagaaa acggttgggg ttcgcggcgt gttttctgat
     1801 tttaatgagt tctgtttccg ttttccaact tccccgcaga ctcgactcac attggacaga
     1861 tcacatcaaa cagttgcgcg aacagggcca tgtccgtatc cccattaatc cgcccggatg
     1921 gagcgtggat ttgtatgaaa cacccacttc acccgatgat taaccgtccg ccgttttctt
     1981 atgaaaaaac atcacatcct ctccttcatc gacaagacag gatatggcct tgaaatcggt
     2041 cccagttaca atccccttgc ccccaagaga ctcggttata atgtccgtac gcttgattat
     2101 ttatcacagt ccgatctaat cgaaaaatat tcgcgtcttt ctgtcccggt cgcggagatt
     2161 gaacccgttg atttcgtctg gtccggagaa ccctactcgg aattaatggg aggtcattca
     2221 ctttttgact gggtcatcgc ctcacatgtc atcgagcaca tgccggacct gatcggattt
     2281 ctgaagaact gcacctccgt cttaaaggaa gatggaattc tcgcgattgt tgttccggac
     2341 aagcgttatt gcttcgatca cttccgcccc ccctcttcgc ttgcgcaaat catcgacgca
     2401 caccaagcga agctgacgca cccctcagcg gggatgatcg cggagagcta tttgaataag
     2461 gcgtatcgaa atggagaggg catttggaac ctgctcactc gcggaccgtt gcgtttccaa
     2521 cacacggtca gcgaaacaaa aaacgcgatg gcgctccgag agtatactga tgtacacgca
     2581 tggagtttca caccccactc gttccgtcta gccatacatg acctcgtttc tctcggacac
     2641 cttgatttga aggaggtttc gtttcgtatc acgtccggct ttgaattcct cgctgtcttc
     2701 cggaaaggca aatcgccccc gtcaagttct cgcttgtgct tggcgaagcg ggcgcattcc
     2761 gaactctcgc cttctcttct tctcgctata cttgaacggc tgggtgtact tttttatatg
     2821 cctcttttct tcgtcaaaag atggctgtcc ccaaaagacc gacgccaagg gtaaaaaatt
     2881 cacggttttc tgaaatacgc aaacaaaaca taacccgccg atcatttttt acgcccaacg
     2941 aattcgattt catcaatcgg cagccccaac caatcccatt tgagcggagc ccggttggcg
     3001 aaaaatgaca agattcggcc gagccaaggg gtaatccaga caaaacggat ccggagggag
     3061 attttttcaa aaagaaccct tccgccccac tgatatgccc gttgctcgca cagtaattcc
     3121 ggtgtgtata cgctgaattg attacagtgt aaatgccacg ctgccattgg attccggaag
     3181 tgtggcaccg taaatcgcag tacccctccc gtcttcaaga tccggtgaat ctcccgaacc
     3241 gtccggtcat aatccggcac atgctccaga aaatgctcca tacaaacctc atccaccgtg
     3301 gcgtcctccc atggccaggg gaaaactgta aggtcataca aaaaatcagg ctggccacaa
     3361 gcatccacat tcacatatcc agacttcacc ttctcgccgc atcccagatt gagtttgaat
     3421 tcgtttccca ttctgaacgc cttccctttg ccggtttatt tattcagtaa cgtgtgctag
     3481 tatatctggg ttttgaagtc tggaaaagat gtttcgttga cacggatgga cttttgcccg
     3541 ggagtcgtat gttgatttcg cttgtcctgt tgacgtggaa ccgttaccgg tttctcgatt
     3601 tatgccttgc gtcgctgttc gcaaaccttt ccggtgcatt cgactaccaa attcttcttt
     3661 tggataatgg ctcgacggat cttacgcctc aagttttgaa ccgttatcga aataaacccg
     3721 gagtgaccct tctcgccaat gcggaaaatc ggggactgag cgcatataag acgctcttca
     3781 ataaagccaa ggggaaaatc atcatcgaag tcgatgacga tatcctcgat tttccaaaag
     3841 attttgacca aatcatggtg gactatttgg cggcttaccc ggattatggg tttttggccc
     3901 tcaatgtcat tcaggacgaa cggacgaatg gcgcgaaacc tgatccttct cattatgcgg
     3961 aagacacccg gggcaacaaa acggttgaac aggggcctgc cggcggttgg tgcgcggggt
     4021 tccgtcgcaa agactaccgt gcgatttggc tacctttcaa tttgatcccg ttaagcatga
     4081 agcgatgtga ggacggcacc ttaaccgccc tgtttcgtag gctgcggggt ttaaaagcgg
     4141 gtgtaatccg cgaggcggtc tgtcttcatg cctgcggccc ctattacgcg cagcaatatg
     4201 ggcagacgga acgggagatt caaaaataca tggccgcagg attaacggaa cttgccgatt
     4261 cgtatattca gacacaaaaa ggcgtttaat ttcttctctc tgttcttgtg aggtttatcc
     4321 ttatgtcgat cccaacaacc ctccgatcag tatccgtacg cgctcttctg atgttaacgg
     4381 tttgttcggg tgtcctgtgt gcaggtcttt acgtccggat ggcgcggctg accgttggtt
     4441 tgcacctgac tccccatccg accggcggca tttcctgtac atgggaagac gattcgggag
     4501 catcccatac tctggagacc tgttcgaccg aaaccggaca ggtctcgttc ccgatcccca
     4561 tccaggccaa actcttccgg tttcattttt tccctagcca tgagccgtat cgaataaacg
     4621 ggctatccct ctttcatctt cggttgctga gcgcgcaatg gcttaataat atgctcaccc
     4681 cggaacatcc cgtctatact caacaatata tgggcaaacg ggactgtctc gaaatcatcg
     4741 ccaccgaggg aacatcgtta acctatcccc ggttcttttc cacccttctc cacttcgcca
     4801 gaagactccg cacagtcacc cctgttttgc tattcggcct gttttgtcta ccgctcggcc
     4861 taaaactccg attttgcggc gtacaccgcc ccttttctct cccgcgaggt ctttgcctcc
     4921 cacatgagga ggcgccgttg agaaaaggag atcgttcggt ttttattttt ttcgtcgcgg
     4981 ccctccttct gtgcctgccg aaccctgtcg caccaaccac cccgggatgt gatgcatctt
     5041 ggtcgtggtt gcttaaccat ttcgccctga acccctcagg gatcgggacc tcttttttct
     5101 tcacttacgg tccactggga ttcacgatca cacctgaagc gatcggaaac aatgcctttt
     5161 gggctctggc cttctctctg ctgtatatcc tcctgttcag cgcaacgatc catatcattc
     5221 tgacgcaaca cgaaacgccc tccgcctgtg cctgccctct attcctctgc atccttctcc
     5281 tgccgcccaa cactgaatgg cgctgggcct tcctgataaa cctgatgctg gcggcctgtc
     5341 tctttttccc gccggaacag acacgcgcac gcgcagttta ttccgccgta accggagcgg
     5401 tgatcctcta tgcgtcgttc atcaaattct ccttagccat caccgcgggg ctcaatgtgc
     5461 tcatggtcct tcttcttctc cccccatgtc agcgccgcga cttatggttt ttcggggttc
     5521 cgttggcagg cggattcttg gtgggcctcc tcttcgcacg ttggttttta ttctcgtctt
     5581 tttcatccat cttgacttgg gcacgtctct cctgggaaat cgcttccggg tataacacgg
     5641 ctatggtcac acaagtcaca tggcttgaac tcatcatccc tgcaattctt gtggccagtt
     5701 ttcttgtcct tctctttccg cttcattcat tctcccttca gcgtttcaaa cgaatggcac
     5761 tggtttcaac gaccctgttt ttcgctttca aatacagtat gacccgcgcc ggcatcggta
     5821 ccctgcgtgc cgtaacctat gtggttccct gtatgactgg catcctcatg ctgtttgcgg
     5881 attctctttg gcgcccgaac cttgcccgac tcttccgttt ccaacttgcc gtatgcgtag
     5941 gactttcggt cttaaccggt gttttcctac agttgccgat catcggcttc aattacaaaa
     6001 acctccttga ttcaacccgt ctgaatcaat gtatcgccca tgcgcgaacc cggtcggacc
     6061 aagcattgct cgatgtgcga cttccgcctc actggatccg gaaaatcggc aacaacacgt
     6121 tcagttcgta tccgacagaa ttgacctacg ctcccgcaaa ttcactgcgg ttcattcctt
     6181 tcccgatcac gcaagggtat tccgcataca cgcaggatct tgatgtccac ggcgcctcgt
     6241 tgttttccgg cccccaagca cccgaatggt tgctttgcgc attcagcaag cctgaccagc
     6301 ggaacggagt cgttgacacc ccggcggtct ggaacgctgt tcgaacccac taccgtcccg
     6361 aagacgtgaa cgaacacggt gttcttcttc aacaccggcc ccacaccgcg atcccacaat
     6421 ggagatgtgt gcgacaacaa actgccgcag taggagagtg gatcgatctg accggaaagg
     6481 acatgaccaa cacctgtctg tcaatcaagt ggtcgccaac cttgctggga aaagggttag
     6541 cccttctctt ccggaataca caatgctcct ttactctaat ccgggtggac ggtacaacac
     6601 atcggtggcg tctggtgccc gacaccgcaa gcactccctt tccgatcggg gccatcccct
     6661 tcacggatcg cgaattcccc cggattttcg acaacccgga ttcagcgcct ttccccgtgt
     6721 cacgcatcaa attcgaatgc gagcaccctt ggtattattc gcggcaaata acgctctcct
     6781 tttttcagta accttttccg ataaagtctg tttactccat acaaacgcac gatcctttta
     6841 agataggcta gcaacaaaaa tctgcctgcc tgcggcttgc ccggttcgga cggttggcgt
     6901 atactgcccc acatggaagc cgatctggaa gtctatctca ccgaacgccg caggcttgtc
     6961 gaagaacacc tctcggcctg cctgcccgaa gccacgccac gcaacaaacg actgattgaa
     7021 gccatgtgcc atgcggtgct ctccggcggc aagcggctgc gcccgatcct ctgcctcgcc
     7081 gcggccgaag cctcgggcgg cagcttgcgt gacgccctct gccccgcctc cgccgtggag
     7141 ttgctccaca cgtacacgct tgtgcatgat gacctgcctt gtatggacaa cgacccgatg
     7201 cggcgcggac agcccaccgt gcacgcgaaa ttcggcgagg cgctcggggt gctgaccggc
     7261 gacgccctgc aaaccctggc cttcgagatt ctcgcgcgca caccggaaaa acaggccggc
     7321 accctgcagc ggctcgtcac cgaactcgcc tccgccgcag gacaggccgg cgtgatcgcg
     7381 ggccaggccg aagacatccg ttgcgcggcc gcgcccacgc gcgcagacat cgagtatgtc
     7441 ttccgccata aaaccgccga cctcttccgc acggcttgcc gtctcggcgc gatcgccgcc
     7501 aacgccgcgg cacccgacct tgaacgcctg accgaatatg gagacggcct cggattcgcc
     7561 ttccagatcc tcgacgacct gctcgatgcc cacgaggcca agacgcgggc cgaaccggaa
     7621 ctctcctgtc tcgcggtcat gacggtcgcg gaggcgcggg agtgggcgga aaatctcacg
     7681 caacaggctg tcgcggcact ggcaggcctg cccggcgaga ccgcgccgct cgaagccctc
     7741 gcgcgtcacc tgctcggccg cctcgcctga gaaccgcggc ccggccgacg cgccgccacc
     7801 ctaacgcgaa gacgggtcta ttcgccgtac acgcgcacga tgcgttcggc gagtgccgca
     7861 cgcttgctgg cgttccagac cacaagggtg acgcgatacg cgccgggctt ccggtacacg
     7921 tgtttcggct gggcctcaag acagggcagc ccgtccccga aatcccagag ccgttgcgcg
     7981 attccctctg gatccaccga tgtgtcgcgg aaagcgaccg gctcgcccgc gcgcgccgtt
     8041 tccggcgcgt caaacgccgc aaccggtgcg gcacccatct ccccgcaggt cgtgagcgtc
     8101 acgttggtct tgttgcccga aaccagattg tcctcccaca ccaccgcgtc acccagatac
     8161 gtccggatgg acgcccccgc gttgttggcg atcacattgc ggatgaaggt gagatggttg
     8221 acattcttcc ccagaatctg gaaagccatt ccgccgttat ccgtgatctg gttgccgtcg
     8281 aacgtgacgt gggtggtgtg gttgttgatc cggatggcgt gtccgtccgc ccccttgtag
     8341 accaccgaag gatggccttt ctgggtgcgg gtgaaaagat tgtcatagaa atagaggctg
     8401 tggatatggt tgctcgccga aggcccctga agctgcatgc cccacatcga gcagcagacg
     8461 aaccggttat gtgcccaaag catgtggtgg gatgcgccgg agacggaaat tccctcgatc
     8521 tggccgtaat ggatgacgtt gtctgtgacg atcccgtagg gtcccccacc gccctcgatg
     8581 ccgtaagcct tccacgttcc gcccgcatcc gccacgagat tacggcgcac ggcacagtgc
     8641 gaactgacga tgctcaaccc gtgatcgacc acgttgtcct caaccaaggt ccgcacgctt
     8701 tcgctgtgca cttcgatcgc gaatccgata ccacccgagc gcgtgaccac attctcgcgg
     8761 atcaccgcgt cggaagcccc gttgttcgtg aaaatgccgc cgcgaccggt ccggtcgatc
     8821 acgttccgct cgatcacggc ccgcagcgat cggtccgaca cgcggatccc cgcgccccaa
     8881 gggtcttccg gcgcgatgtt gcagatcgtg ttgtcgcgga tcaccgaatc agaggtttgc
     8941 gataagagaa tcccgtgcgg cccgaacgtc cccgtgtcca cgaaatcccg gatcgtgagg
     9001 tgatggaggc aaagggtgtc gcacttcatc gcaaccaccc cctgggtcgc caacgggttg
     9061 ctgccgccat cgagtgtcag gtggcacacc tgcaccccgg agacatcacg catccgcaca
     9121 aattcatgcg gtttttcccc cccgaaagcc agaatggtcc gctcctgccc cgctccttgc
     9181 agtgtgacgc cggagggcgg ggtgagcggg ctgaacaggc gcaacacgcc tgcggggagc
     9241 gttaccgtgt cccccggttg cgccgcggcc aacgccttgt tgatggcctc ggcgtcatcc
     9301 agcaaatcat ccggcacgca gtcggcaccc accggacggg ttgcggcggc cgcgatgccg
     9361 accgtcatgg ccgcacccca caggatcatc cgtatcgtta ccgcacaacg aatccgcatc
     9421 tctcctcctt tttacactca gtgccgtttt ttcttcggcg tctttttgga aagatccaga
     9481 aacggcgccc gccggagcga gacctcgggg atacccgcct ccccatcccc gtccgaaggg
     9541 gtgccggttt ccggctccgc agtgccgacc gcagtccaca cgccccgggg cgcagggcgc
     9601 agcaaatccc aaagggattg cttcacctcc gccacgcccg gatcgtccgg cgtcaccgca
     9661 gaaacccgga tcggcgtaac gccggtctcg cgcacaaagc gcggcaagta ttccgcagcg
     9721 gccgccacat ccatcttgtt ggccagcacg agggaggggc ggtcgagcat ttcggcgttg
     9781 tgctgcgcga tctcgttccg cagcgccaga tagtccttcc acggcagacg attgtccgtc
     9841 cccgccatat cgaccacgaa aatcagcaca cgcgaccgcg cgatgtgttt cagaaaactg
     9901 gtgccgagac cgacccctgc agcggcccct tcgataatcc ccggcacatc ggccacgcga
     9961 agctgggaga aatccggata catcagcgtt cccacgatcg gattgagcgt ggtgaacggg
    10021 taattggcaa tcttcggacg cgcgccgctc agacaggcga gcaacgagga ctttccggcg
    10081 ttcgggaacc cgagcaagcc cgcatccgca atggttttca gttccaaccg gagccggaac
    10141 tcctcacccg gctccccggg cgtgtgttcc gttggcacct gatgcgtcgc ggttttgaaa
    10201 tgcacgttgc cgaggccgcc tttgccgccc cgcgcgatca caacctcttc gtcgtggccc
    10261 acgacatccg cgatcagttc cccggtctcg gcatcaaaca cgcttgtgcc gcacggcacc
    10321 cggaccaccc ggtcgcgccc ccgccgtccg tacatcttct ggccgcgtcc cgccaccccg
    10381 ttttcggcga acagttgcgg cgcgaaggag agaccgatca gcgaatcgac atcacggctg
    10441 ccccggaaaa gcacatggcc cccacggccg ccatcgccgc cgtcggggcc ccctctggga
    10501 acacacgcct cccgtctgaa actcgcgctt ccgtcccctc ccttaccggc gcggacatgc
    10561 accacgacac gatccacaat ctttcttgtc ttcatgccgc agtccgccgc agacccggat
    10621 cagatcactc cgccgcaact gcggggatga tgctgaccac cgcgccgcct ttttggaatt
    10681 tgacggtgcc gtcctttaaa gcgaacaggg tgtaatcgcg tccacatccg acatgctttc
    10741 cggggtggaa ccgggttccg cgctgccgga ccagaatcga tcccgcgcgc acgacctgcc
    10801 cgtcataccg cttcacgccc aaccgcttcg cattgctgtc ccgcccgttt cttgatgctg
    10861 cgcctgccat gtgtcacact cctccggtta ggcgatcgcc ttgactgtgg ccaccgtcag
    10921 ttcctggcgg tgccccatcc ggcgcgagta ccccttgcgc cgttttttct taaagttaat
    10981 cagtttcttg ccgcgtttgt ggccgatcac ggagagcgtc accttcgcgt ccgggacata
    11041 cggcatcccg accttcagtt cctgcccgtc cgagagcgcc aacaccgtct ccaacaccac
    11101 gtcctgaccg gcctcggcat ccagacgctc aatctcgatc gtgttgccca cggccacacg
    11161 atactgcttg ccgccggtct ctaaaaccgc atacgcatcc atgttttgac ccttcatcac
    11221 tggggaaccg agcggttccg cacctttcca aatcggaaca aaaggatttt actttaccgg
    11281 atcgcccccg cgactgtcaa gcgggccagc ctctttattt ttggcggcac cgagcgcgtt
    11341 ctcgatggtg tcgagaagtt tctccgcatt gaagggtttg gaacggtact cgaagacccc
    11401 gatccgcttg gactcgatcg cggtctcgtc cgacgcgtag gccgtcagga tgatcacggg
    11461 gatatccggc ttgatcgcct taacctcgcg caaaagggtc atgccgtcca tgcgcggcat
    11521 gcggaggtcg gaaatgatca gatcgatctc cgttttgcgg atcacgtcca gagccgtcac
    11581 cccgtcttcc gccacgacca catcatatcc gtttgtgttg agaagccgct gaaagaacat
    11641 cagcagcccc ggctcgtcgt caacgatgag gattctggcc ataaccaatt ctctttctcc
    11701 tgataggcgc ctgtaggcat cacacacgtt cccgtccctc actcagggca tcgcgctctc
    11761 cactgcacga aggtttggga ctgaaacctg tcatctaccg ttacacatca ctgtaagggg
    11821 catccgtttt cccgtcaaca acgaaaagcc gtgcgcccgt gacaggttcg cgacttcgca
    11881 cactccctgt cacgaactga ttaccgtgcg cccgaagatt catggtccgt tcggctccgt
    11941 gaataaagcc gattgacacg atatgctgga agtgcgaaac tattatccgt gtgaagcttg
    12001 ttatccgtgg ctggatgcac ttgtggcttc acaacaaaag tgcgaaatat cgtgagcgtt
    12061 tgcccaatgg accttactgg aacatcacgc gatgaccgca caagaacgga tgctctgaaa
    12121 attccgtgtc ccgcgggggc gtgcgtgtca tgcgcgaccg gcgtggtact ggctgctttt
    12181 tgctgggctc gggccgacgg tccgttccgc gtcttgccgt acctgcagaa cccggcgact
    12241 gacgcgatca cggtgatgtg gttctcggag acctccacca ccggtcaggt tgccttctgc
    12301 cagaccgggg tatggtcggc gccgcttgaa gttcccgcag tgctggccag cgcactggac
    12361 taccactcca gcgagacgaa ttcgcccccg cccctccccc atccgcccct cccctattta
    12421 caccgggtca ggctgacagg gctgctggcc ggaaccgcct acgagtacgt ggtcacgcag
    12481 gacgaggtgt cgatcaccaa ctcgttccgc acggcaccgc cgcgagacag cgccgtgcgc
    12541 ttcgttgtct attccgactc cgagacagaa ccggaatcga ccggctcccg agagaagtgg
    12601 cccgagccga acggtaacgc cgaccgcctg tatccagtcg accagacacg cgggtatcag
    12661 gaaaacctcc agattatggc tgcccgcaac ccggacttta tcgtgatcgc cggcgatctt
    12721 gttgaggcgg gcggcgagca acgggactgg gacgagttct ggcgtcacaa cgccggtgtt
    12781 ctgggtgatc tggccagcac cgtgcccatc atgcccgcgc tgggcaacca cgaatactac
    12841 ggcggtacgc gcggcggtta cagccagccg acctctgagg tgagcgtcgc caaatacctg
    12901 acctatttcg agctgcccgc caacggcgcg gcaaaccccg cccataacgg acggtattac
    12961 cgcttggact acggaccggt gacgctggtg gtgctggacc tgtgtaatgg cgatgacacg
    13021 gaccccgcca aagacaccag cacgtacctg aagacggccg acggctgtca ggcgccggac
    13081 ttcaaccccg gctcgccgca gtacgcttgg ctggaggagc agctcgcctc ggcccagacc
    13141 aacagtccgt tcacctttgt ggcctttcac cagaccccgt attctgtcgg gccgcacgga
    13201 aaggctgacg aatcacagtc gggtgtgccc gtgcgtgtcc tcacgccgct tttcctgaaa
    13261 tacggcgtgg atgccgtact ggccggacat gatgagatgt acgagcgctc cgtcgtgaca
    13321 gggatagagc agttgcccgg cggcggcacg cgccccgtca cgctcagctt ctatgatatc
    13381 ggcatcggcg gcgacaggtt gcgggagccg ttttctggct tggttaacga caaacaggtt
    13441 tttctcgcgc acgacgacgc gcctgagatg tggtcaggcg cgaggcttgt gagcggcggc
    13501 aaacattacg ggcacctgga ggtcaacgtc cgggtgaacg gacagggaaa atgggaggcg
    13561 gttctgaccc cggtctacgc gtttccgctc atggacagcg ggggcacggt caccggcttc
    13621 gagcggcgga cgtacgccga cgaggtgacg atcataagcg cttcggcccc ccttctcggg
    13681 cagaaggggg caattgtgac ggaatggtga aaaacggaaa gagaggagat cagcatgaaa
    13741 aacaatcggt tcgttggctg gatttgtgtg acagccgtgt tcgcggccct agtcgcacag
    13801 gcggctgtca cgccggcccc tgtgccggcc ggggcgttaa aaatgaaacg gatgatgaaa
    13861 ctgtccaaca cgggaagcgt c
//
LOCUS       DAOSLE010000071        13813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4531_length_13813_cov_8.418375, whole genome shotgun sequence.
ACCESSION   DAOSLE010000071 DAOSLE010000000
VERSION     DAOSLE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13813
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4531_length_13813_cov_8.418375"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1394
                     /gene="tig"
                     /locus_tag="PK576_06285"
     CDS             102..1394
                     /gene="tig"
                     /locus_tag="PK576_06285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE61238.1"
                     /translation="MKVESKSVGPCQVKVFVKAEAEETRGEYEKVLKLFMKEGRVPGF
                     RQGKVPREVIKRSFHNEIQEEVRSRLFRSLYKPALEQKKIAFVALRDVGDMLFSPETG
                     ISFSMTVDVKPEFELPRYKKIPVVFEEPVVTEEQVDGQIQQLREAFAQFKEAGADDEI
                     RDGDMVRIDFAGTVDGQSLAELDPAAKALAGESGQWVLVQEGRFLPEILQALRGLKQG
                     AETTAQVTFGADHPITALQGKTATYSVTVREIRARSLPADEELCGRLKVASIDEMRTQ
                     TREGMMEAARKSERIRREQTVIDFLLKQADFDLPESEEAEEINTILDQMMGEAQSRGL
                     TREDLEKSRGQIIENATTTARRQLRLRYILARIAEKEALTLSPEEIDSRIAEFANASR
                     STPAQFRAQVEKNGRMDALREHMLNLKTIQFLLDQAKG"
     gene            1407..2018
                     /gene="clpP"
                     /locus_tag="PK576_06290"
     CDS             1407..2018
                     /gene="clpP"
                     /locus_tag="PK576_06290"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOE61239.1"
                     /translation="MESPQANMLIPIVVEQTGRGERAYDIYSRLLQDRIIFIGTAITD
                     EVSNVIIAQLLFLQAQDATKEISVYINSPGGSVTAGLAIYDTMQFISCDVATYCVGQA
                     ASMGAVLLAAGSKGKRFSLPNARIMIHQPWGGAEGKATDISIAAKEILRLKDRLNEIL
                     AHHTGKSVDVLSNDTERDYFMSADEAKTYGLVDDVLTQSLAEK"
     gene            2022..3272
                     /gene="clpX"
                     /locus_tag="PK576_06295"
     CDS             2022..3272
                     /gene="clpX"
                     /locus_tag="PK576_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOE61240.1"
                     /translation="MAKRPPKKAMLTCSFCGRGDRADAVVIPGPDVNICSDCVDICTR
                     ILAENNATPASSAALPVLHVPPPGEIKTFLDQYIVGHDRTKKILAVAVHNHYKRLNQP
                     LTKKDAFADVEIEKSNILLIGPTGCGKTLFARSLARMLDVPFAIADATTLTEAGYVGE
                     DVENILLYLLQAADMNVARAERGIVYIDEIDKIARKTENVSITRDVSGEGVQQALLKI
                     IEGTVSRVPPQGGRKHPQQEYISINTRNILFICGGAFVGLDTIVRQREGRQTIGYGEP
                     KRKGRDAQSRDGHPVPLAVQPEDLIRFGLIPEFVGRLPVLTTMSEMTEDDLVRILTEP
                     RNCLVRQYQKLLDMGGVKLDFDEDALREIAALAMKRKTGARGLRAVMEEFMMEIMYDA
                     PGNPKLKAIRVTAEMIRSRFGNAA"
     assembly_gap    3298..3397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3478..4836)
                     /locus_tag="PK576_06300"
     CDS             complement(3478..4836)
                     /locus_tag="PK576_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE61241.1"
                     /translation="MFTRRNLLRLVLFVLCALAAAGLMPEAWRRGSVLLPGLSPLLSL
                     GGALAARAAGVLTLLGLPLLILPLFAGRFFCWRLCPMGFAAETVSRINRRNTGWIRKI
                     PFVGKGLALLILGSAAAGWPVLIWTDPLSVFNGFFAAARQPLTWLSAATASGFILILL
                     ASLIVPNLWCHRLCPLGGLQEWLSQLGKSLRSARCAKEGDEIPPLLTHARTGRRVFLG
                     FALGGVSGSLYRAYAPRRVTTDTDCGGTHSCGGRHGHGPGEGEAGQRRRMGRGSGAGL
                     PIRPPGAVQQAFNALCARCGNCMTACPYGLILPDLGTTGPDGLFTPVLAFRNLNPQQE
                     QFCFQECTACTQACPTGALRPLTVARKQQTAIGLAHICRNKCIAWKKGEYCVVCQEFC
                     PYQAIREVERNGVMCPIVDADKCRGCGACESQCPALPIAVTIRGRDPQTVLAHPSPAL
                     HT"
     gene            complement(4848..5753)
                     /locus_tag="PK576_06305"
     CDS             complement(4848..5753)
                     /locus_tag="PK576_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOE61242.1"
                     /translation="MKPVKRRSFLKTGVTAAGTALVQTPLAAADAASPAATVIVVHGT
                     DIPKMLEAGIAKLGGWQAFIRTGMDVVIKPNVAWNSKPEQGGNTHPDLVRACVLAAEK
                     QGAATVSLPENTCHPEKATFQASGIAAALEGTRGRLYRPAKQDYGEAEIPKGKIVKRA
                     FVPKDILNCDCLINMPVAKHHSGATLTLSMKNWMGSVSNEDRRAWHRDGLHQCIADIN
                     TFLRAKLVVIDATRIMLTKGPQGPGELAHPYEIILSTDPVAADAYAVTLFKKTPFDVP
                     HVRIAHEMGIGCGDLAKIRVERVEA"
     gene            complement(6088..7065)
                     /locus_tag="PK576_06310"
     CDS             complement(6088..7065)
                     /locus_tag="PK576_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE61243.1"
                     /translation="MSWQDWRWQLRHAVTGTSPLLRAYGVDPDTAERVHRRYPVFLTP
                     YALAQLTPGEPQDPLNRMALPSAAELFREPGMTPDPFGEQGRRVACCPGLKQRFPDRV
                     LVMAHDRCALACRHCTRKGLLGRASVMRTDAELAAAVVWVRAHPRVREVLLSGGDPLL
                     LPDTRVARLVRAFAALPQIDAVRIGTRVPVTLPMRITPNLARRLGAFRKVWVNTQFNH
                     VREITPEAEEACARLVDAGIPVSNQSVLLKGVNDSVQALFDLCAGLQRIRVRPYYVFL
                     CDPVAGLAPFRVPLRTARQIERALAVRLGGLALPRFVMDQPGSMRKIPI"
     gene            complement(7087..7932)
                     /locus_tag="PK576_06315"
     CDS             complement(7087..7932)
                     /locus_tag="PK576_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE61244.1"
                     /translation="MKNRIVLTAMAVGLCCAGCFKHREAPPRPVTASSLEEAAQKRAE
                     IECLNLAGQEISALPRELAAMPKLSVLWLRGAKGLTEFGVLSDLKALKRLDLSALGSK
                     AVPPEVCGLKELEHLYLADNALTALPPAIGGLSKLSYLNLDRNPLSSLPNELGALSEL
                     RWLRLNQNALTSLPLSCANLKRLQRLYLRGNRFDHVPACLKDLPLLEDLCLSQNPIKT
                     LPDWLTALPKLRQLDLDGCPLGALPANLSGWKRLQVLSLVRCPLPDEERARISAALPD
                     THIAF"
     gene            complement(8009..8428)
                     /locus_tag="PK576_06320"
     CDS             complement(8009..8428)
                     /locus_tag="PK576_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61245.1"
                     /translation="MRLCLTFFSLALGCAMVVKADARVMITEHRDVNGKSTYWYITQE
                     RLAQTPEWKPDVSLPLPVEKAAGKATEWLKKRTTKFTEWDIVSISLGKIWDSEIKNKW
                     YYSVSVNGTVQVDGIKANSFFSVIVLMDGTVVEPTSE"
     gene            complement(8597..8731)
                     /locus_tag="PK576_06325"
     CDS             complement(8597..8731)
                     /locus_tag="PK576_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61246.1"
                     /translation="MKLHENQEFPLAFQKNPSSAIPCVRGEMIDPDPDTDTDPDLDET
                     "
     gene            9206..9871
                     /locus_tag="PK576_06330"
     CDS             9206..9871
                     /locus_tag="PK576_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOE61247.1"
                     /translation="MRISNDNDLAEACRKLRCADKRLAEIINHVGPCTLKPHTPDFHF
                     LADAIIGQQIATKAAATISARFKQLFKGNKPTAKGFLNIPKEEVLSAGVSGQKYGYIL
                     DLAQRINSRKLRLASLSKMSDSGVREKLTEVKGIGVWTADMFLLFGLGRLDVFPLHDF
                     ALRQAMARVYDVPADDHGALLRIANRWAPYRSVGTWYMYRSKNAQQGSQRSGTRSRAR
                     GRE"
     gene            10053..12299
                     /locus_tag="PK576_06335"
     CDS             10053..12299
                     /locus_tag="PK576_06335"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61248.1"
                     /translation="MILVCLNGLCLWAAAGLLEQQELTGLDEIVFVKRHTYNANHYYT
                     EFINSTWMPGGNICVLNLKTGAVRELLPQMTGGVFGRFDLSFDARRVVFDWKPGPQEG
                     CRIFEVNIDGTGLRQLTFPQDDEAELSAAYRKSPKYHHGTDDMHPCYLPDGGIAFVST
                     RCQYGILCDPADEFTTTVMFRMDGDGKNMRQLSNSSVSEACPVMLEDGRLMYTRWEYV
                     DKGAVAVKCLWAMRPDGTASQEIYGNDIAVPTTMLYGRPIPGMADAFVFLGTPHYPHN
                     GFGTVVRLDMSKPIRTELPMTYITADVDIKTEGGFHFRDSAGGWQRDPEGKGRLFKDP
                     YPLSAALYLVSHKPAGEVWRSPAGYGLYVLNEAGEVAEIYRDPAISCFLPYPLRARSV
                     PPVVHSPVNSALAAAGEAVCLVQDVYHGLTGVPRGAVRWLRVLEQVPRPWAAQMADNS
                     DEYDQQHIVISKDTHLGLKVQWGVVPVDPDGSAHFKVPAGRNLFFQALDADYLMLQTE
                     RTYVNYMPGEVRSCIGCHETPQSAADYQRKDRRRRARPSAFGRAPDVPGPQPNEERGQ
                     RTLDYMTDVQPLFDRHCISCHGESAGEPGKLDLRGKLTRKFCVSYEALVPERRKGSFY
                     RGLLGTVIGENHPKTGNVDYLPPGTLGARTSVLAAMLGRGKIVLADTANQARAASLAQ
                     SHVKALARLTPEEFLRLANWIDTNCQYYGSYWGRRDLKHKDSPFFRPSATFEQAISRE
                     FPWPGGMR"
     gene            12299..13642
                     /locus_tag="PK576_06340"
     CDS             12299..13642
                     /locus_tag="PK576_06340"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOE61249.1"
                     /translation="MAIDGKALALACFKAGVAGVAPDRLVRDHVRSDERTLTICGQEF
                     DPSRIERIFVVGAGKASGLMALALEEMLGDRVIGGAVVVKHGHAVPCRKIRIMEAAHP
                     YPDQAGVDATQKIMRFCEEAREGDLMLCVWSGGGSALLADAPEECSVEEVARLSEVLV
                     TSGADIGEINAVRKHLSRVKGGQLARLAWPARVVSLILSDVVGDPLDVIASGPTVADP
                     TTFGEALAVLRKYRIEAQTPPQLLQVLNNGAAGLLPETPKADDPALARTVNSIIGNNR
                     IALEHVAQYAQSRGLSVRIVTDRLEGATAEAAATIVRAACEAQSDTSLAKPACLLFGG
                     ETTLRVMGCGTGGRNQHLALQAARLLRGRKGITLLAGGSDGTDGPTEMAGAVVDGETC
                     VAAERANVDAAAYLRAFDSYAFFKKIGGHVFTGPTRTNVMDLIVVILGADASPAP"
BASE COUNT         2732 a         3855 c         4451 g         2675 t
ORIGIN      
        1 aactgcgcgg agggacgggt tttatttgcg cattcccatc ccgatgcgga catgttatat
       61 taaacgtctt ttccaaacgg gtgcagacga aaggtttccg gatgaaagtc gaatccaaaa
      121 gtgtcggtcc gtgtcaggtc aaagtcttcg tgaaagccga ggccgaggag acgcggggcg
      181 aatatgagaa ggtgctgaag cttttcatga aagaggggcg ggttcctggt tttcgccagg
      241 gcaaggtgcc gcgcgaggtc atcaagcgct cattccacaa tgagattcag gaggaggttc
      301 gctcgcggct ctttcggtcc ttgtacaagc ctgcgctgga gcagaagaaa atcgcgtttg
      361 tggcgctgcg ggatgtgggg gacatgctgt tttctcctga gacgggtatc agcttttcca
      421 tgaccgtgga tgtgaaaccg gaattcgagt tgccgaggta taaaaaaatc ccggtcgttt
      481 ttgaagagcc cgtggtcacc gaagagcagg tggacggcca gatacagcag ttgcgtgaag
      541 ccttcgccca gttcaaggag gcgggtgcgg acgatgagat ccgcgacggc gacatggtgc
      601 gcatcgattt cgccggcacg gtggacgggc aatcgctcgc tgagctggac ccggccgcga
      661 aggcgctcgc cggcgagtcg ggacagtggg tgctggttca ggagggccgg tttctgccgg
      721 agattctgca ggcgctgcgg gggttgaaac agggcgcgga gaccacggcg caggtgacgt
      781 tcggtgccga tcacccgatc acggcactgc agggcaagac cgcgacgtat tcggtgacgg
      841 tccgcgagat ccgcgcgcgc tctctgcccg cggatgagga actgtgcgga cggttgaagg
      901 ttgcgtccat cgacgaaatg cgcacgcaga cccgcgaggg catgatggag gcggcgcgta
      961 agtccgagcg gatacgccgc gagcagacgg tcatcgactt tctgttgaaa caggcggatt
     1021 tcgatttgcc ggaatccgag gaggcggagg agatcaacac catactcgac cagatgatgg
     1081 gcgaggcgca gagtcgcggc ctgacacgcg aggatctgga gaagagccgc gggcagatca
     1141 tcgagaacgc gacaacaacg gcgcggcggc agttgcggct gcgctacatt ctggcgcgca
     1201 tcgcggagaa agaggcgctc acgctcagtc cggaggagat cgacagccga atcgccgaat
     1261 tcgcgaacgc atcccgctcc acccccgcgc agttccgtgc gcaggtggaa aaaaacggac
     1321 ggatggatgc gctgcgcgaa cacatgctca acttgaaaac catccagttc ctgcttgatc
     1381 aggcgaaagg gtgaggagga cgaaacatgg agtcaccgca agcgaatatg ctgatcccga
     1441 tcgtggttga acagaccgga cggggcgagc gcgcatacga catctactcg cggctgttgc
     1501 aggaccgcat tattttcatc ggcaccgcca tcacggacga ggtcagcaat gtgatcatcg
     1561 cgcaattgct cttcctccag gcacaggatg ccaccaagga gatttcggtg tacatcaact
     1621 cccccggcgg ctcggtcacc gcgggtctgg cgatctacga taccatgcag ttcatcagct
     1681 gtgatgtcgc cacgtactgt gtgggccagg cggcgagtat gggcgcggtg ctgctggctg
     1741 cgggcagcaa gggcaagcgc ttctccctgc ccaacgcgcg catcatgatt caccagcctt
     1801 ggggcggcgc ggagggcaag gccaccgaca tcagtatcgc ggcgaaggag atcctgcgcc
     1861 tgaaagaccg gctcaacgag attctcgccc atcataccgg caagagcgtc gacgtgctgt
     1921 cgaacgatac cgaacgcgac tatttcatgt ctgccgacga ggccaagacg tacggactcg
     1981 tggacgatgt gctgacccaa tctcttgcgg agaaatgatc catggcgaag cggcccccga
     2041 aaaaagcgat gctcacctgt tccttctgcg ggagggggga tcgtgccgat gcggtggtca
     2101 tccccggccc cgacgtgaac atctgcagcg actgcgtcga catctgcacg cggatccttg
     2161 cggaaaacaa cgcgaccccc gcatcgtctg ccgcgttgcc cgtgctccac gtcccgccgc
     2221 ccggcgagat caagacgttt ctcgatcagt acatcgtcgg ccacgaccgc accaaaaaaa
     2281 tactcgcggt cgcggttcac aaccactaca agcgcctcaa tcagccgctc acgaaaaagg
     2341 acgccttcgc ggatgtggag atcgagaaga gcaacatcct gctgatcgga cccacggggt
     2401 gcggcaagac gcttttcgcc cgctcgctcg cccggatgtt ggatgtgccg ttcgcgatcg
     2461 ctgacgccac gacccttacc gaggccggat acgtgggcga ggatgtggag aacatcctgc
     2521 tctacctgtt gcaggcggcg gacatgaatg tggccagggc cgaacgcggc atcgtgtaca
     2581 ttgatgagat cgacaaaatc gcgcgcaaga ccgagaacgt ctccatcacg cgggacgtgt
     2641 ccggcgaggg tgtgcaacag gcgctgctca agatcatcga gggcacggtt tcgcgcgtac
     2701 cgccgcaggg cggacgtaag catccgcagc aggagtacat ctcgatcaac acgcgcaaca
     2761 tcctctttat ctgcggcggt gcctttgtgg ggttggatac gatcgtgcgg cagcgcgagg
     2821 gacgccaaac gatcggatac ggggagccga agcgcaaagg gcgcgacgct cagtcccgcg
     2881 acgggcatcc ggtgccgctc gccgtgcaac cggaagacct gatccgcttc gggctcattc
     2941 ccgaattcgt gggccgtttg ccggtgctga ccaccatgag cgagatgacc gaggacgatc
     3001 tggtgcgcat cctgaccgaa ccgcggaact gtctggtgcg gcagtatcag aaactgctcg
     3061 acatgggcgg ggtgaagctc gacttcgatg aggacgcgct gcgcgaaata gctgcgctgg
     3121 ctatgaagcg taaaaccgga gcacgcggac tccgggccgt gatggaagag ttcatgatgg
     3181 agatcatgta cgatgcgccc ggcaatccga aactcaaggc catccgcgtc acggcggaga
     3241 tgattcgctc ccggttcgga aatgccgcgt agtcggtctc cctcccgagg cgtccgtnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc gaggcgtccg tatggtggat
     3421 ccgcatctgc gaaatccgta tcatctgtgg ataaaaaacc gatgcgtcgg cccttgctca
     3481 cgtatgcagc gcgggtgagg gatgcgcgag caccgtctgc gggtcgcgcc cgcggatcgt
     3541 gacggcaatc ggcagcgcgg ggcactggct ctcgcacgcg ccgcacccgc ggcatttgtc
     3601 cgcatccacg atcgggcaca tgacgccgtt gcgctcgacc tcacggatcg cctgataggg
     3661 gcagaactcc tggcagacca cgcagtactc gcctttcttc caggcgatgc acttgttccg
     3721 gcagatgtgg gcgagtccga tagcggtctg ttgcttccgc gcgaccgtca acgggcgcag
     3781 tgcgccggtg gggcaggctt gtgtgcaggc ggtacactct tggaagcaga actgctcctg
     3841 ctgcggattg aggttgcgaa aagccagcac cggcgtgaac agaccgtctg gaccggttgt
     3901 tcccagatcc ggcagaatca ggccgtaggg acaggctgtc atgcagttgc cgcaacgggc
     3961 gcagagcgcg ttaaaggcct gctggaccgc acccggcggc cggatcggga gacccgcccc
     4021 tgagccgcgc cccatgcgcc gtctctgccc tgcctccccc tctcccggac cgtggccgtg
     4081 gcggccgccg caagaatgcg ttccgccgca gtccgtgtcg gtggtaacac gacgcggagc
     4141 gtacgcacgg tagagcgagc cggagacgcc tccgagcgcg aatccgagaa aaacgcggcg
     4201 tccggtccgg gcgtgcgtca gaagcggagg gatttcgtcc ccctccttcg cgcaccgggc
     4261 tgaacggagg cttttaccaa gttgcgacag ccactcctgc aagcccccca gcgggcaaag
     4321 ccgatggcac cagaggttcg gcacgatcag actggccagc aggatcagga tgaacccgct
     4381 ggcggtggcg gccgaaagcc aggtcaacgg ttgtcgcgct gccgcgaaga atccgttgaa
     4441 cacactcaac ggatcggtcc agatcagcac cggccatccc gctgcggcgc tgccgaggat
     4501 cagaagcgcc agacccttgc cgacaaacgg gatcttgcgg atccaaccgg tgttgcggcg
     4561 gttgatacgg ctgactgtct cggcggcgaa gcccatgggg cagagtcgcc agcagaagaa
     4621 gcgtcccgcg aaaagcggca ggatcagcag cggcagtccg aggagcgtga gcacgcctgc
     4681 ggcgcgggcc gcgagggcgc cgcccagact gagcaggggg cttaggcccg gcagcagcac
     4741 actcccgcgc cgccacgctt ccggcatgag tccggctgcc gcaagggcgc acaaaacgaa
     4801 caacacaaga cgaaggagat tacggcgcgt gaacatgtcg tgtctcgcta cgcctccacc
     4861 cgttccaccc ggattttggc gagatcgccg cacccgatcc ccatctcatg cgcgatgcgg
     4921 acatgcggca catcgaacgg cgtttttttg aacagcgtga cggcgtaggc gtcggctgcc
     4981 accggatcgg tgctcagaat gatctcgtac ggatgggcca gttccccggg tccctgcggg
     5041 cctttggtga gcataatgcg ggtagcgtcg atcaccacca gctttgcgcg cagaaaggtg
     5101 ttgatgtcgg cgatgcactg gtgcaggcca tcgcggtgcc acgcgcgccg gtcctcgttc
     5161 gagaccgagc ccatccagtt tttcatcgag agggtgagcg tcgcaccgct gtggtgtttg
     5221 gccaccggca tgttgatcag acagtcgcag ttcagaatgt ccttggggac aaaggcgcgc
     5281 ttcacgatct ttccctttgg gatctcggct tccccgtaat cctgtttggc ggggcggtag
     5341 agtcgacccc gtgtaccttc cagcgcggcg gcgataccac tggcctgaaa tgtggctttc
     5401 tcgggatgac aggtgttctc gggcagggat acggttgcgg cgccctgttt ttcggcagcc
     5461 agcacacagg cccggacaag gtcgggatgg gtgttgccgc cctgttccgg cttcgagttc
     5521 catgccacgt tgggtttgat caccacgtcc atgccggtcc ggataaacgc ttgccagccg
     5581 cccagtttcg cgatgcccgc ctcgagcatc ttcgggatat ccgtgccatg gaccacaatc
     5641 acggtggcgg caggagatgc ggcgtctgca gccgcaagcg gtgtttgcac cagcgcggta
     5701 ccggcggcgg tcactccggt tttcaagaaa gagcggcgtt ttacgggttt catatgggtt
     5761 ccctcttgca gtctcacagc cttacagcct tttgggttgc gggcgccgcc cgcgttggtt
     5821 tgtatggagc caagatacca catttgggaa atcaggcgcg atcttttttc gtgaccgtac
     5881 tgccgattta ggcatatccc cgtttctctg gccgttaagc ctataaacgg caggtggacg
     5941 tgcgaatttt gggtaaaggc ttgtaaacat tcgaaccatg aaacatgaaa tcgggaatgg
     6001 ggcaagcgtc tgcttgcccg gacgggcagg gagtcgggag cgcgggcgcc ctcgcccgca
     6061 actcggttcc gggtttcggg ggttcggtca gatcggtatt ttacgcattg atccgggctg
     6121 atccatcacg aaacggggca acgctaaacc gcccagacgc acggccaggg cgcgttcaat
     6181 ctgccgtgcg gtccgcaacg gcacgcggaa cggggcgagc cccgcaaccg gatcgcagag
     6241 aaaaacgtaa tacggccgca cgcggatgcg ctgcaggccc gcgcagaggt cgaacaacgc
     6301 ctgcacggaa tcgttgacgc ccttcagcag cacgctctgg tttgataccg ggatgcccgc
     6361 atccaccaga cgcgcgcagg cctcttcggc ttccggggtg atctcgcgca catggttgaa
     6421 ttgcgtgttg acccacacct tacggaacgc acccaagcgg cgcgccagat tgggggtgat
     6481 ccgcatcggc agggtcaccg gcacacgcgt gccgatccgc accgcatcga tctgcggcag
     6541 tgcggcgaag gcacgcacca gccgcgccac ccgcgtatcc gggagcagca gcggatcacc
     6601 gccggaaagc agcacctcac gcacgcgggg atgcgcccgt acccagacta cggccgccgc
     6661 aagttccgcg tcggtgcgca tgaccgacgc gcgtccgaga agtcctttgc gcgtgcagtg
     6721 gcggcaggcc agcgcgcatc ggtcatgcgc catcaccagt acgcggtcgg ggaaacgctg
     6781 tttgagaccg ggacagcacg ccacccgccg cccctgctcg ccgaacgggt cgggggtcat
     6841 gcccggctcg cggaacagtt ccgcggctga ggggagggcc atacggttca gcggatcctg
     6901 cggctcgccg ggggtgagtt gcgcgagcgc gtagggggtc aaaaacaccg gatagcggcg
     6961 gtgtacccgt tccgccgtat cgggatccac tccgtacgcg cgcaacagcg gggaggttcc
     7021 ggtgaccgca tgccgcagtt gccagcgcca gtcctgccaa gacattgggc gttgagggct
     7081 cacgggtcag aacgcgatgt gggtatccgg aagggccgcg ctgatgcggg cccgctcctc
     7141 atccggcagc ggacagcgca ccagcgagag gacttgcagg cgtttccacc cgctgaggtt
     7201 tgcgggcaac gcgccgagcg gacagccgtc gagatcgagt tgacgcagtt tcggcagagc
     7261 cgtgagccag tccggcaggg ttttgatcgg gttctgactg aggcagagat cctcaagcag
     7321 cggcagatcc ttcaggcaag ccggtacgtg gtcgaaccgg tttccccgca ggtaaaggcg
     7381 ttgcagccgt ttgaggttcg cgcaagacag cggcagggag gtcagcgcgt tctggttgag
     7441 acgcaaccag cgcaactcgg acagcgcccc cagttcattc ggaagcgaag acagcggatt
     7501 ccggtccaga ttgagatagc tcagtttgct cagcccgccg attgccgggg gcagggcggt
     7561 tagggcattg tccgcgagat agaggtgctc cagttccttc agaccgcaca cctcaggcgg
     7621 cacggctttg cttcccagag ccgagaggtc cagccgtttc agggctttca gatccgacag
     7681 aaccccgaat tcggtaagac ccttggcccc gcgcagccaa agcacggaaa gtttcggcat
     7741 cgcggccaac tcgcggggca gcgccgagat ttcttggccc gcaaggttca ggcattcgat
     7801 ctccgcccgt ttttgcgcgg cctcctccag actcgaggcg gtgacggggc ggggcggtgc
     7861 ctcacggtgc ttgaagcaac ccgcgcaaca caaccctacg gccatcgctg tcagtacgat
     7921 ccgattcttc atacgcgttt ccagtccttg ttaatgagaa ccagcataca cgaaacgccc
     7981 ggtggttggg gaggtttttt tggtggcctt attcgcttgt tggctcgaca acggtaccat
     8041 ccatcagaac gataacactg aagaacgaat ttgctttgat gccatccacc tgaacggttc
     8101 cattcacgga aacagagtaa taccatttat tctttatttc cgagtcccat attttgccta
     8161 gactaatgga gacaatatcc cattcggtga attttgtggt gcgctttttt agccattctg
     8221 ttgcttttcc tgccgctttc tcaactggca ggggcaaaga aacgtcaggc ttccattcag
     8281 gggtctgcgc aagccgttct tgtgtaatat accagtaagt tgacttgccg ttcacgtcac
     8341 gatgttcggt tatcatcaca cgcgcatcag ctttgacaac catcgcacaa ccaagtgcca
     8401 agctaaaaaa cgtcaaacag agtctcattg cccatcctcc ttgttaccaa gatcaacccg
     8461 cacattccca tcagcagcaa gttcaccgaa agggtacgct tcttttgggg gcagcgcaag
     8521 ggtttttggg gaacgccagg ggggattcct gattttcatg aaggttcata tgtttcaggg
     8581 tttctggttt ctggtttcag gtttcatcca aatcgggatc ggtatcggta tcgggatcgg
     8641 gatcgatcat ttcaccacga acacacggga tggcactgct cgggtttttt tggaacgcca
     8701 gggggaattc ctgattttca tgaagtttca tgtgtttcag ggttccatcc aaaccgggat
     8761 cggggtcggg ataacgcgtg tgcacatgcg cgcaggcctc aatcgtgttt caatgggggc
     8821 gcggcttgac aggttcgggg aggagttcgt aaactgaacc gcacggatag ggtcgccatc
     8881 gcggagtgcg ttcagtggca ctgcattgtt gggcgggaag caaggcagcg agaacagagg
     8941 ggattgcgcg tgaagtatga ggatttgaca ttcaaggtgc caaagcaatg cgatgactat
     9001 gacgaatgct caaccttcgt taccgaatcc attgagacga ttatccagac tgcgattgat
     9061 gcaggtagaa acaagattgt aatcaatgcc cacgcagacg cgaagcaaca tccgattagc
     9121 cccggcgccc gcatgaaacg gtgaagcccc acaacatacg gtcattggag agccttgctc
     9181 tacattgggt attcatgagc tgacaatgcg catttctaac gacaacgatc tggctgaggc
     9241 atgccgcaag ttacgttgcg cagacaagcg gcttgctgaa attatcaacc acgtcgggcc
     9301 gtgcacgctg aagccgcata caccggattt tcattttctg gctgatgcga ttatcggtca
     9361 gcaaatcgcc acgaaagctg ccgccacgat ttcagcccga ttcaagcagc ttttcaaagg
     9421 aaacaaaccc acggcaaagg gctttctcaa cataccaaaa gaagaagtgc tttcggctgg
     9481 cgtgtccggg caaaaatatg gctacattct tgaccttgcc caacgtataa acagccggaa
     9541 gctgcgtttg gcctcgctct ctaagatgag cgattccgga gtacgcgaaa aattgacgga
     9601 ggtgaagggc atcggggtct ggaccgctga catgtttctt ctttttggcc tcggcaggct
     9661 ggacgtcttt cctttgcacg acttcgccct aaggcaggca atggctagag tctatgatgt
     9721 gccagccgat gatcatgggg ctttgttgcg catagccaac cgctgggctc cttatcgcag
     9781 tgtgggaacg tggtatatgt atcgaagtaa aaatgcccaa caaggttcac agcgtagcgg
     9841 cacacgctca cgtgcacgag gacgcgaatg aggttcgata taaattcagg aggccgtgtt
     9901 tcgaggacaa ggaggagcta aaagatcgta gttccgggaa cagcgtttat ggtaaaataa
     9961 tcgtacctca agccgcatgg aacgactggc gaggcatctt gggagcgggg tgcatgtata
    10021 ggagaaatcg tatgcgtgaa cggatgcgct gtttgatttt agtttgccta aacgggttgt
    10081 gtctgtgggc ggcagccggt ttgctggagc aacaggagtt gacggggctg gatgaaatcg
    10141 tttttgtcaa gcggcacacg tacaacgcca atcactacta tacggaattc atcaacagca
    10201 catggatgcc gggcggtaat atctgtgtgt tgaatcttaa aaccggcgcg gtgcgcgaac
    10261 tgctgccgca gatgacgggc ggcgtgttcg gccggtttga tctttcgttt gacgcgcggc
    10321 gtgtggtttt cgactggaag cccgggccgc aggaaggctg ccgcattttt gaggtcaaca
    10381 ttgacggcac gggattgcgg cagttgacgt ttcctcagga cgacgaggca gagctttcag
    10441 cggcttaccg caagagtccc aagtatcatc acggcacaga cgatatgcac ccgtgctacc
    10501 tgccggatgg cgggatcgct tttgtctcga cccgttgtca atacggcatt ctgtgcgatc
    10561 cggccgatga gttcaccaca acggtgatgt tccgcatgga tggcgacggc aagaacatgc
    10621 ggcagctttc caacagttct gtcagcgagg cgtgtccggt gatgctggag gacgggcggc
    10681 tgatgtacac ccggtgggaa tatgtcgaca agggcgcggt ggcggtgaaa tgtttgtggg
    10741 cgatgcgtcc ggacggcacg gcctcgcagg agatctacgg caacgacatc gcggtgccga
    10801 caaccatgct ttacggcagg ccgatcccgg gtatggcgga tgccttcgtg tttctcggta
    10861 ctccgcatta cccgcacaac gggttcggca cagtggtcag actggatatg tcaaagccga
    10921 tccgcacgga gttgcccatg acttatatca cggctgacgt ggatatcaaa accgagggcg
    10981 ggtttcattt ccgggacagc gctggcgggt ggcagcgaga tccggagggc aaggggcggc
    11041 tctttaaaga cccgtatccg ttgtctgccg cgctctatct ggtctcgcac aagccagcgg
    11101 gcgaagtgtg gcggtcaccg gcagggtatg gcctttatgt tttgaacgag gcgggagaag
    11161 tcgcggagat ctaccgcgat cccgcgatat cctgtttcct gccttatcct ttgcgggcgc
    11221 gaagtgtgcc gccggttgtg cattcgcctg tgaacagcgc attggctgcg gcgggggagg
    11281 ctgtctgtct ggtgcaggat gtataccacg ggcttacggg agtgccgcgc ggcgcggtca
    11341 ggtggctgcg cgtgctggaa caggtgcccc ggccgtgggc ggcgcagatg gcggacaaca
    11401 gtgacgagta tgaccagcag cacatcgtga tcagcaagga cacgcatctg gggctcaagg
    11461 tgcagtgggg cgttgtgccg gttgatccgg acggctcggc acatttcaag gtgccggctg
    11521 ggcgtaatct gtttttccag gcgctggatg ccgactatct gatgttgcag acagaacgga
    11581 cgtatgtgaa ctacatgccg ggcgaggtgc gttcctgtat cggttgccat gaaacgccgc
    11641 aaagcgcggc ggattaccag cgtaaagaca ggcggcgcag ggcgcggccg agcgcgttcg
    11701 ggcgggcgcc ggatgtgccg ggcccgcagc ccaacgaaga gcgcggtcaa cgtacgctgg
    11761 attacatgac ggacgttcag ccgctgtttg accggcactg catatcctgt cacggcgaaa
    11821 gcgcggggga gccgggaaag ctggatttgc gcggcaagct gacacgcaaa ttctgcgtct
    11881 catacgaggc actggttccg gagaggcgca aaggctcttt ttaccgcggt ttacttggca
    11941 cggttatcgg cgagaatcat cccaagaccg gcaatgtcga ttacttaccg ccgggcacgc
    12001 tgggggcgcg caccagtgtt ttggcggcga tgctgggacg gggtaagatt gtgctggcgg
    12061 atacggcaaa ccaggcgcgg gcggcctctc tggcgcagtc gcatgtcaaa gcattggcac
    12121 ggttgacccc cgaggagttt ttgcggctgg ccaattggat cgacaccaac tgtcagtatt
    12181 acggctctta ttgggggcgg cgtgacctga aacataaaga ttcgccgttt ttccgtccgt
    12241 cagcgacgtt tgagcaggcg atcagccgcg agtttccatg gccggggggg atgcggtgat
    12301 ggctattgac ggaaaagcgc tcgcgctggc gtgcttcaag gccggtgtcg cgggcgtcgc
    12361 cccggatcgg ctggtgcgcg atcacgtcag atctgatgag cggacgctga cgatctgcgg
    12421 gcaggagttc gatccaagcc gcatcgagcg gattttcgtg gtcggcgcgg ggaaggcgtc
    12481 tggactcatg gcgttggcgc tggaggagat gctgggtgac cgggtgattg gcggtgcggt
    12541 tgtcgtcaag catggccatg cggtgccttg ccgaaaaatc aggatcatgg aggcggcgca
    12601 tccttatccg gatcaggcgg gcgttgacgc cacgcaaaag atcatgcggt tttgcgagga
    12661 ggcccgcgag ggagacctca tgctgtgcgt ttggtcgggc ggcggttccg cgctgctggc
    12721 cgacgcgccg gaggagtgct cggtggagga ggtggcgcgc ctgtcggagg tcttggtgac
    12781 gtccggggcc gacatcggcg agatcaacgc ggtccgcaag catctcagcc gggtgaaggg
    12841 tggacagttg gcgcggctcg cctggcccgc gcgggtggtg agcctgattc tttcggatgt
    12901 ggtcggtgat cctttggatg tcatcgcttc gggcccgacc gtggcagacc ccacaacgtt
    12961 cggggaagcg ttggctgttc tccggaaata ccggatcgag gcgcaaacgc ccccacagct
    13021 tctgcaggtg ttgaacaacg gcgcggcagg ccttctgccc gaaacgccga aagcggatga
    13081 tcccgcgctg gcacgaacgg tgaactcaat catcggcaac aaccggattg cgctggaaca
    13141 tgtcgctcaa tatgcgcaga gtcgggggct gtcggtgagg atcgtgacgg accgcctcga
    13201 aggggcgact gcggaggcgg ccgcaacgat cgtgcgggcc gcgtgcgagg cgcagtccga
    13261 cacgagcctc gccaagcccg cctgtctgct gttcggtggc gaaaccacgt tgcgggtcat
    13321 gggttgcgga acgggtggcc gtaaccagca tctcgcgctt caggccgcac ggctgctgcg
    13381 cgggcgcaag gggatcactc tgctggctgg cggttcggac ggtacggatg ggccgacgga
    13441 aatggcgggc gcggtggtgg acggggaaac gtgcgttgcg gccgaacggg ccaatgtgga
    13501 tgcggcggcg tatctgcgcg cctttgattc gtacgcgttc ttcaaaaaaa tcggtggtca
    13561 tgtgttcacc ggcccgacgc ggaccaacgt catggactta attgtcgtga ttctgggcgc
    13621 tgacgcatcc ccggccccct gaccggtgag gttcttggct tcatccaaat cggtatcggt
    13681 atcgggatcg ctatcgggat cgctatcggg atcggctgtt tcaccacaga acactttctt
    13741 gggtttcacg gtttcatggt ttcagggttt cgggtttcat ggtttcgggg tttctgggtt
    13801 tcatccaaat cgg
//
LOCUS       DAOSLE010000072        13742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4558_length_13742_cov_8.418280, whole genome shotgun sequence.
ACCESSION   DAOSLE010000072 DAOSLE010000000
VERSION     DAOSLE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4558_length_13742_cov_8.418280"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            502..2187
                     /gene="rpsA"
                     /locus_tag="PK576_06345"
     CDS             502..2187
                     /gene="rpsA"
                     /locus_tag="PK576_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOE61250.1"
                     /translation="MMAAALDQQMNQFVAGSIIKGTISGIKGNEVLVDIGYKSEGIVP
                     ANEFEDVTQLKIGDEIDVLLMELESESGMVSLSKARADEKLRWDAVLAKYSEGGVVTG
                     TIRTKVRGGLIVDVDGVDAFLPGSQVDVTPVHDPDPYLNQSFEFKVIKISNERRNIIV
                     SRRELIEERMADKKRELLGTIEKGQMRHGHVKNITDFGAFVDLNGLDGLLHITDMSWG
                     RIKHPSEMLKVGQELDVLILDVDMERERVSLGLKQATPNPWENIEGTFPVGSRLRGKV
                     VNLVPYGAFVEIQPGIEGLVHVSEFSWTKRVARASDVLNVGDEVDVVVLSIDAANQKI
                     ALGIRQTEANPWDTVQDRYPVGSRVTGKVRNFTTYGAFVELEEGIDGMIHVSDMSWTR
                     KINHPSEVLQKGQQVEAVVLEVNPADQRISLGLKQASDDPWNTITSRYAIGQIVKGKV
                     SKIASFGAFVELEDGVDGLVHISQISDQHVEKVKDALKVGQDVEARIVKIDRDERRIG
                     LSIKAVSMDESEIAELTRDLSTVDLKPGENLVGLAAAFDDAFAQSEEWQPGAE"
     gene            2322..3590
                     /gene="tyrS"
                     /locus_tag="PK576_06350"
     CDS             2322..3590
                     /gene="tyrS"
                     /locus_tag="PK576_06350"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE61251.1"
                     /translation="MNILDTFKARHLFEDMTSPGLAKALDKPLTVYAGFDPTSDSLQA
                     GNFVTIMALAHFQRAGHKVIALVGGATGLIGDPSGKSKERNLLSAEQVEHNLVGIREN
                     LSRFLDFNPDSANPAILVNNYDWFKSFTFIDLLRDVGRYFRMGVMLSKDSVKKRMESD
                     DGMSFTEFCYQIMQGYDFYHLHRTFGCTLQIGGSDQWGNITAGTELVRRICGEEVFGM
                     TFPLVCDSTGKKFGKSEGNAIFLDARKTSYYEFYQFFLRTLDADVIRYLKIFTFLPMG
                     RIAELEQAVAAAPEKREAQQVLAEEVTRTVHGENGLAVAQKATGVLFGGAIEGLAAAE
                     LEAIFVNVPSATLPADHVLGKPAFEVIAAAGMVASKGEARRLVQQGGLYINNARVTGI
                     ERVFDASDLCEGRLTVLRSGKKSFFLLKVR"
     gene            3698..5254
                     /locus_tag="PK576_06355"
     CDS             3698..5254
                     /locus_tag="PK576_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOE61252.1"
                     /translation="MWRIVCLIVAVRAGFGAPAPEAALFLQTGTPTPENPVDAPIFRK
                     LKRHGITPVYCTDAVFLRRAYLDILGTLPTADEARAFLADTSMHKRTALIDRLLERDE
                     FADYWAMRWSDLLRVKAEFPVNLWPNAAQAYHRWIRTAMRDNKPYDCFARELLLSNGS
                     NFRVGPVNFYRATPDRSPEGLASAVALTLMGARTEHWPSNTLADLAVFFSQVRYKPTR
                     EWKEEIVFWDPEQPLTTTAGRLPDGTSVRLEPGRDPRTAFAEWLITPQNPWFTRALVN
                     RVWYWLLGRGIIHEPDDIRSDNPPSHPDLLKRLQREFVRSGYDLRKLYRLILTSRTYQ
                     FSPVFPSGRVESAAPLFAYYPLRQLDAEVLIDVINKVTGTSDLYTSAIPEPFTFIPAN
                     KPAVALPDGSITSPFLELFGRPARATGLASERINRALPPQRMHLLNSSHIQRKLETSP
                     VLRRMFRGKRGSRAMIEEAYLTTLSRFPSATEIGTVERYLRSGVVKEDAAGVDLIWAL
                     INSDEFLYRH"
     gene            5268..6599
                     /locus_tag="PK576_06360"
     CDS             5268..6599
                     /locus_tag="PK576_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009103731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOE61253.1"
                     /translation="MRERKSVTRRDLLRTGLCGAAVAVWPGRTVRGQAAPAARKATAK
                     SVIQVFLWGGMSHTDTWDPKPDAGYDYTGAFKDVIPTNVDGIRLGALFPKLAQQADKF
                     SILRSMTHRNNGHETAAYLMQTGHMPGQRLAYPAIGAVFALFKGEAAGYTGLIPPYVV
                     LTQPQGRFSEAGFLGSRCKPFATGGDPNAPRFEVEGVIARGITDQRQEARRGLLSSLN
                     TLGNTLSGHPELAAAREAEKRAYDLILGQGRDVFDLSKEEATIRDRYGRHTFGQDCLA
                     ARRLVEAGVPYITINYPGGWDTHSNHFQTMNRQCPQLDQGLAALLQDLAERGLLESTL
                     VWCCGEFGRTPKVDWQPPWNGGRNHHGNVFSVLVAGGGFRGGHLVGASTAKGEEVEER
                     PIYPIDLLGSIYELAGIDTEAALPHPEGADARVLDMREAGAKSAGRLKEIM"
     gene            6718..8784
                     /locus_tag="PK576_06365"
     CDS             6718..8784
                     /locus_tag="PK576_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61254.1"
                     /translation="MLRSWAFRALLAVLPGFLCAQQPAYIGFVFPAGGRQGTVFRCTL
                     GGQALDGVNGVIFTGSGVTGRVIEYNRHMNSQEVQLLREQLDEIRAMPKARRDAQVTN
                     LASRLEMLIGDYVQQPQCASIANLVTVEVAVERGTPPGPREIRLVTPRGVSNPLAFHV
                     GALPEVTSEPLPTSPKQILGKEAQSLRSTSGRKRGAAGKGRGRRGMVEREAEMIQMVS
                     GMAGRGALTDVDDREVRIMPPCTVNGQIASGSVDRYRFDGRSGQRLVVTVQARALVPY
                     LADAVPGWFQPVLVLCDAQGREKAYNDDYRFHPDPVLYCELSANGEYVLAIHDAIFRG
                     REDFVYRMTIGELPFVTSVFPLGGQAGVDAVIEVKGFHLAETRLTPDLRTRLPGLHTL
                     TVAGSGGLRSNPFVFEISTLPDGMEHEPNNTLKRAQPVTLPIVMNGTIGMPGDLDVFT
                     FRGRAGQEVVAEVRARRLGSPLDAWLKIMDDQGRTLAWNDDQEDPGSGLDTHHADAYV
                     RVTLPADGAYAVQLRDTQHQGGEAFGYRLRLSEPQPDFDLRVVPSRVVVRRGGETPVT
                     AHALRKDGFAGPITFRIREPAAGFEVRGATLTGTQTVARLMIRARQPSDSGVPVPIVI
                     QGVSGEGEGAIIRDAVAAEDCMQAFLWRHLVPAQEFLVLMIPGPPRPSGKPGFPAASR
                     RREKPE"
     gene            8885..9868
                     /locus_tag="PK576_06370"
     CDS             8885..9868
                     /locus_tag="PK576_06370"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE61255.1"
                     /translation="MSARKLTAKITGVGKALPEGRLTNADLEKRVDTNDAWITQRVGI
                     RERRIAAEGEHTSDFAIRAARDALALSGLKPEQIDLILVGTSTPDMIYPSVACQVQQA
                     LGASNAGVLDISAACTGFIYGASLAWGMIQSGALRHVLVIGAEKNSAIVDWSDRDTCI
                     LFGDGAGAAIFSEHRSNTDGVLSAKLGGDGSFGGLLHLPNSGCRSFPEENGRFIRMQG
                     NEVFKQAVRNMYECAVCVLDQAGLSSQEVSLLIPHQANVRIIDAVAKRLGLPKEKVFR
                     NIAEYGNTSAATIPIALCEALEQGRLKKGDVLVIDAFGAGLTWGAMAIRWA"
     gene            complement(9875..10915)
                     /locus_tag="PK576_06375"
     CDS             complement(9875..10915)
                     /locus_tag="PK576_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE61256.1"
                     /translation="MTIDGRPVTLKPGMTVYEAAQGIGIRIPVLCHADGVVPAGGCGL
                     CTVEDTAAGGKLLPACATRADAAMSIVTDSEAVRNFRRTALELLLSNHPADCEAPCRM
                     ACPSGLPVQTLMARIRSGDWQSARDLARAHPFACGGAAPCEKACRRALLDGAVAICAL
                     HRWLAGETAGEACSRVPGGTQARPRFRSRMPDLNVAALAALTPETGPRRLPPETHEPT
                     PEAAAFEAGRCMRCGCLKPTKCRLRELCAEYGANPRAFDHAPRPVIRETGRNGFRFDA
                     SRCILCGLCVRITRQCGAGELGPAFHGRGFEARIGPPLGRVWDDLPDSVLQACAAACP
                     TGAMAFETNAVV"
     gene            complement(10926..11345)
                     /gene="rnk"
                     /locus_tag="PK576_06380"
     CDS             complement(10926..11345)
                     /gene="rnk"
                     /locus_tag="PK576_06380"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOE61257.1"
                     /translation="MTKRTLYISEMDRHKLEELVNVAHLSHDRDQGDLKSLQNELQRA
                     LVVEPEQVPPNVVTLHSRVRLREVKTQTVMEVTLVMPSEANVDAGKLSVLSPVGTAIL
                     GYAEGDTIEWMVPAGHRRFVIEKLLYQPEASSRSSRK"
     gene            11795..>13742
                     /locus_tag="PK576_06385"
     CDS             11795..>13742
                     /locus_tag="PK576_06385"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61258.1"
                     /translation="MIRNKQILSLMLLTAFLAVSARGVYMVHEFYLPLPEAQVLKAFN
                     TLDGLGQVKAPIDSVTSIVVTGPDTIIHYDHWEDGYEVDINNPAQPTTRIWGDGNDAN
                     GIPPGYASDPMGLPQGAVISLRNNVNLPRNPSQILYDGRDRITASKALAVSRALWPVS
                     VGTVLSEAVEVTATIDHGTHYIAPVGEDVDAFEMFEQVGLAVMADQDNTVVTIDKDGP
                     GGAAPVTVTLNQGESHYVSGGVLKGAEVTATKPVQVHLLTGDTGATYETRWYTLFPVE
                     DWFSRYVTPIGSSANGQACFVFLYNPGPETITIEAASRIGTESITVPAGEVYRWQVPQ
                     NSGAMFSSAGDEPFSAIAAAGAHPNANAAYDWGFSLVPQDALSSTAVVGWGPGSSDMT
                     RNGSPVFVSPATATRIYVDYNGDGQGPLTDPNGGKYDKHYDLAALQSQTLFDPDGDQT
                     AMRIYTIDGALFSASWGQDPDTARPGNPFLDMGSTVLPLPEPKLVKTARIVNDVDPAG
                     PSLGDTIEYRIVIDNKGLLPLGNTLVLDTLPSQLSYVTNSTTLDGEPLPDDTVGSTLF
                     PLDEDGYTIPVILRAGTSIFTYQTTIVGTGSIVNRSAVPQYEVYDETGADVPPPAGSH
                     PCTVEFVDAGGNPVTVYREGAPIYL"
BASE COUNT         2580 a         4261 c         4471 g         2430 t
ORIGIN      
        1 cccccgacat ccagctccca aagctggcgc actaccaggc tgtgcaacgc cccgaaagca
       61 tatgcgtggg ggagtgtagg acattcatgg gcaacgtgca atagtcgagt tggcggcaac
      121 gggttttcag ttggatgatt tctgcccgtg agggtgatct cgcgggtgag attcgatacg
      181 agcgagcttt tgggttgaac gctgaacgtt cggatttccg gatacccgtt ttttggtttg
      241 tcccgcgaag ggattttttg ctataaagaa cgtcttttgt ccccaacagg acaaaaaagg
      301 ggcacgtgca gccggccggg tggccggaga agcgacggcc ccggaagtgc aacacctaac
      361 ccaacgtctg acgccgggac tcgttcccga atcccatatg tgcgcggaac cacgcgtgcc
      421 gcggcggcag actggaattc cttacacatg gcaattcgta aacccacggc cgaaactccc
      481 aaaaccggtc ccatgtacga catgatggcg gcagcgctgg accagcagat gaatcagttt
      541 gtggccgggt cgatcatcaa gggcacaatc agcggcatca aaggcaacga ggttctcgtt
      601 gatatcggtt acaaatcgga aggcatcgtt ccggcaaatg agttcgaaga tgtgacacag
      661 ctgaagatcg gcgatgagat cgatgtgttg ctcatggagc tggaaagcga aagcggcatg
      721 gtgagcctga gcaaggcccg cgccgatgag aagttgcgct gggatgcggt gctcgccaaa
      781 tacagcgaag gcggcgtggt caccggcacc atccgcacca aggtgcgcgg cggtctgatc
      841 gtggatgtgg acggcgtgga cgccttcctg cccggatcgc aggtggatgt caccccggtg
      901 catgatccgg atccctatct gaatcagtcg ttcgagttca aggttatcaa aatcagcaat
      961 gagcgccgca acatcatcgt gtcgcgccgt gagctgatcg aagagcgcat ggccgacaag
     1021 aaacgcgaat tgctcggaac gatcgagaag gggcagatgc gtcacggtca cgtcaagaac
     1081 atcaccgatt tcggcgcgtt cgtggatctg aacggtctgg acgggctgct ccacatcacg
     1141 gacatgagct gggggcgcat caagcacccg agcgagatgc tcaaggtggg gcaggaactg
     1201 gatgtgctca tcctcgatgt ggatatggag cgcgaacggg tttcgctggg gctgaagcag
     1261 gccacgccca atccgtggga gaatatcgaa gggaccttcc cggtcggcag ccgtttgcgc
     1321 ggcaaggtcg tgaatctggt gccgtacggc gccttcgtcg agatccagcc gggtatcgag
     1381 gggctggtgc atgtctcgga gttctcttgg accaagcgcg tggcgcgcgc ctcggacgtg
     1441 ctgaacgtcg gggatgaggt ggacgtggtg gtgctgagca tcgacgcggc caaccagaag
     1501 atcgcgttgg ggatccgcca gaccgaggcg aatccgtggg acacggtgca ggaccgttat
     1561 ccggtcggca gccgcgtgac cggcaaggtg cgcaacttca ccacgtacgg cgcgttcgtg
     1621 gagctggaag agggcatcga cggcatgatc cacgtctccg acatgtcgtg gacccgcaag
     1681 atcaaccacc ccagcgaggt tctgcagaag ggtcagcagg tcgaggcggt ggtgctcgag
     1741 gtcaatccgg ccgatcagcg catcagcctg ggcctcaagc aggccagcga cgatccgtgg
     1801 aacaccatca ccagccggta cgcgatcgga cagatcgtca agggcaaggt ttccaagatt
     1861 gcctccttcg gcgcctttgt ggagctggaa gatggcgtgg acggactggt ccacatctcc
     1921 cagatcagcg atcagcacgt cgagaaggtt aaggacgccc tcaaggtcgg ccaggatgtg
     1981 gaggcccgca tcgtgaagat cgaccgcgac gagcgccgca tcggcctgag catcaaggcc
     2041 gtgagcatgg acgagagtga gatcgccgag ctgacgcgtg atctgagcac cgtggacctc
     2101 aagccgggcg agaatctggt gggccttgcg gcggcctttg atgacgcctt cgcccaaagc
     2161 gaagagtggc agccgggcgc ggagtaagca aaaaagggcg cctggcgaca ggcgccccgt
     2221 cctttttttc gatggaatcg agagaatcga cacactcgat taaatcgatt ctttcgaatc
     2281 catcggttct ctcgacaaca agctatcccc ccctctcccc catgaacatc ctcgacacct
     2341 ttaaagcccg ccatctgttc gaagacatga ccagccccgg gctggcgaag gcgctggaca
     2401 agcccctgac cgtgtacgcg gggttcgacc ccacgagcga cagcttgcag gccggcaatt
     2461 tcgtgacgat catggccttg gcgcattttc agcgcgccgg gcataaagta atcgctttgg
     2521 tgggcggcgc cacgggcttg atcggcgacc cgtcgggcaa atccaaggaa cgcaatctgt
     2581 tgagcgcgga gcaggtcgag cacaacctcg tcggcattcg ggaaaacttg tcgcgcttcc
     2641 tcgatttcaa cccggactcc gccaacccgg cgattctggt gaacaattac gactggttca
     2701 agtctttcac cttcattgat ctgttgcgcg atgtgggccg ctatttccgc atgggcgtga
     2761 tgctcagcaa ggacagcgtg aaaaagcgca tggagtccga tgacggcatg agtttcacgg
     2821 agttctgtta ccagatcatg caggggtatg acttctatca tctgcaccgg actttcgggt
     2881 gcacgctgca gatcggcggt tcggaccagt ggggcaatat cacggccggc accgagctgg
     2941 tgcggcggat ctgcggcgaa gaggttttcg gcatgacgtt tccgctggtg tgcgacagca
     3001 ccggcaagaa attcggcaaa agcgagggga acgcgatctt ccttgacgcg cgcaagacct
     3061 cctattacga gttctaccag ttcttcctgc gcacactgga cgcggatgtg atccgatacc
     3121 tcaagatctt cacctttctg cccatggggc gtatcgcgga gctggagcag gcggttgccg
     3181 ccgcgccgga gaaacgcgag gcgcagcagg tgctggccga ggaagtgacg cgcaccgtgc
     3241 acggtgagaa cgggcttgcg gtcgcccaaa aagccacggg agtgctgttc ggtggcgcga
     3301 tcgaggggct ggctgcggcg gagctggagg cgattttcgt taatgtgccc tccgccacgt
     3361 tgccggcgga tcacgtgctc ggcaaacccg cgtttgaggt gattgccgcg gccggcatgg
     3421 ttgccagcaa aggcgaggcg cgccgtctgg tccagcaggg cggtctgtat atcaacaacg
     3481 cgcgcgtcac gggaatcgaa cgcgtgttcg acgcttctga cctgtgtgaa ggtcgcctca
     3541 cggtcctgcg cagcgggaaa aagagcttct tcctgctcaa ggtgcggtag ggcgggcatg
     3601 cggttgcgta ccggcaccgg gaaatagatt ggctgtcccg ccgtttaagg atacgattcg
     3661 gatatctgtg gtggagtgtg cggatagggg gcttcgcatg tggcggattg tatgtctgat
     3721 cgttgcggtg cgggcagggt tcggcgcgcc agcgccggag gccgcgcttt tcctccagac
     3781 cggcacaccc acacccgaga acccggtcga cgcgccgatc ttccggaaac tgaagcgcca
     3841 cgggattacg cccgtctatt gcaccgatgc ggttttttta cgccgcgcct atctcgacat
     3901 tctcggcaca ctgccgaccg cggacgaggc ccgtgccttt ctcgccgaca cctcgatgca
     3961 caagcgcacg gcgttgatcg accgcctgct tgagcgtgat gagttcgccg actattgggc
     4021 catgcgctgg agtgatctgc tgcgcgtcaa agcggaattt cccgtcaacc tctggccgaa
     4081 tgccgcccag gcctatcatc gctggatccg caccgcgatg cgcgacaaca agccgtacga
     4141 ctgtttcgcg cgcgagctgc tgctgtccaa cggcagcaat ttccgcgtcg ggccggtgaa
     4201 tttctaccgc gccacgccgg accgttcgcc ggaagggctc gcctccgccg tcgcgctcac
     4261 gctgatgggc gcacggaccg agcactggcc ctccaacacg ctggccgatc tcgcggtgtt
     4321 cttctcgcag gttcggtaca aacccacccg ggagtggaaa gaggaaatcg tcttctggga
     4381 tcccgaacag cctttgacca cgacggccgg tcgcctgccg gacggcacct cggtccgact
     4441 ggagcctgga cgcgacccgc ggacggcgtt cgccgaatgg ctgatcacgc cgcagaaccc
     4501 gtggttcacg cgcgcgctgg tcaaccgcgt ctggtactgg ctgctcggcc gtgggatcat
     4561 ccacgagccg gacgacatcc ggtccgacaa tccgccaagc catcccgact tgctgaagcg
     4621 gctgcaacgt gagttcgtgc ggagcggcta cgacctgcgc aagctctacc gcctgatcct
     4681 cacctcccgc acctaccaat tctcgcccgt gttcccgtcc gggcgggtcg agtccgccgc
     4741 gccgctgttc gcgtattatc cgctgcgcca actcgacgcg gaggtgctga tcgacgtgat
     4801 caacaaagtc accggcacgt ccgacctcta caccagcgcg attcccgaac ccttcacctt
     4861 tattcccgcc aacaaacctg cggtcgcgct gccggacggg tcgatcacca gcccgtttct
     4921 ggagttattc ggtcgtccgg cacgcgccac cggccttgcg agcgagcgga tcaaccgcgc
     4981 gctgcctccg caacggatgc atctgctcaa ttcgagccat atccagcgga aactcgagac
     5041 cagcccggtg ttacgccgga tgttccgcgg caaacggggt tcgcgcgcca tgatcgaaga
     5101 agcctatctc acaacccttt cgcggttccc gtccgccacg gagatcggaa ccgtcgaacg
     5161 ctacctgcgc tccggggtgg tgaaagaaga cgcggcgggt gtcgacctta tctgggcatt
     5221 gatcaacagc gacgagttcc tgtaccggca ctgagaagga ggtgtgtatg cgagaacgga
     5281 aatccgtcac gcgtcgcgat ctgctgcgga ccggcttatg cggtgcggcc gtcgcggtgt
     5341 ggcccggacg gacggttcgc gggcaggccg cgcccgcggc gcgcaaggcg accgcgaaat
     5401 cggtgatcca ggtttttctc tggggcggca tgtcgcacac cgacacttgg gaccccaaac
     5461 cggacgcggg ttacgattac accggcgcgt tcaaggatgt cattcccacc aacgtggacg
     5521 gcatccgtct cggcgcgctt tttccgaagc tggcgcaaca ggccgacaag ttttcgatcc
     5581 tccgcagcat gacgcaccga aacaacgggc acgagaccgc cgcgtatctg atgcagaccg
     5641 ggcatatgcc ggggcaacgg ttggcttacc ccgcaatcgg cgcggtcttc gcgctgttca
     5701 aaggcgaggc ggcaggctac acaggactga tcccccccta tgtggtgctg acgcagcccc
     5761 aaggccgctt ttccgaagcc ggattcctcg gttcgcgctg caagccgttc gcgaccggcg
     5821 gcgaccccaa tgctccgcgc ttcgaagtcg aaggcgtgat cgcgcgcggc atcaccgatc
     5881 agcggcagga agcgcggcgc gggctgctct cctcgctcaa cacgctgggc aacaccctgt
     5941 cgggccaccc cgaattggcg gcggcgcgcg aggcggagaa acgcgcatac gacctgatcc
     6001 tcggtcaggg ccgcgacgtt ttcgacttgt ccaaagagga ggcaacgatc cgcgaccggt
     6061 acggccgcca cacgttcggc caggattgtc tcgcggcacg ccgtctggtg gaggcgggcg
     6121 tgccgtacat cacgatcaac tatccgggtg gctgggacac ccactccaac cattttcaaa
     6181 ccatgaaccg gcaatgcccg cagctggatc agggactcgc cgcgctgttg caggatctgg
     6241 cggaacgcgg gttgctggaa agcacgctcg tctggtgctg cggcgaattc ggccgcacgc
     6301 ccaaggtgga ctggcagccg ccttggaacg gcgggcggaa tcaccatggc aacgtttttt
     6361 ccgtattggt cgcgggcggg ggtttccggg gcgggcatct ggttggtgcc tcgaccgcca
     6421 aaggcgaaga ggtggaagag cgccccatct atccgatcga tctgctgggt tccatttatg
     6481 aactcgccgg gatcgacacg gaggccgcgt tgccgcatcc cgaaggggcg gacgcgcgtg
     6541 tgctggatat gcgcgaggcg ggcgcgaaga gcgcgggacg cctgaaagag atcatgtgaa
     6601 agggggccgg gtatgacggc atggaaccaa cccgccgccc acggaacggg aacaagcgat
     6661 gggataacgg gaacggccaa cgctcacgtt gcgggaaggg aaagccgcct gcggccgatg
     6721 ctgcggtcat gggcattccg tgcgctgctg gccgtcctgc cgggattcct gtgcgcccag
     6781 caacccgcct acatcggttt cgttttcccg gcgggaggac ggcagggcac ggttttccgc
     6841 tgcacactag ggggccaggc gctcgacggg gtcaacggcg tcatctttac gggatcaggg
     6901 gtgacggggc gcgtcatcga gtacaacagg cacatgaact cgcaggaggt gcaactgctg
     6961 cgggaacaat tggacgagat tcgggcgatg cccaaagcgc ggcgcgacgc gcaggtcacc
     7021 aatctggcgt cgcgcctgga gatgctgatc ggcgattacg tgcagcagcc gcagtgcgcc
     7081 tccatcgcca atctcgtgac ggtcgaggtc gcggtcgagc gcggcacgcc gcccggcccc
     7141 cgcgaaatcc ggctggtgac accgcgcgga gtgtccaacc cgctcgcgtt tcatgtgggc
     7201 gcgttgcccg aggtcacgtc cgagccgctt cccaccagcc cgaagcagat tctcgggaag
     7261 gaggcccaga gcctgcgcag cacttccggc aggaaaaggg gggcagccgg aaagggtcgg
     7321 ggacggcgcg ggatggtgga gcgggaagcg gaaatgatcc agatggtctc gggcatggcg
     7381 ggccggggcg cgctgacgga tgtggatgac cgcgaggtgc ggatcatgcc gccctgcacg
     7441 gtcaacggcc agatcgcctc gggatcggtc gaccgctacc ggtttgacgg gcggagcggc
     7501 caacggctgg tcgtgaccgt acaggcgcgc gcgctcgtcc cctatctggc ggatgcggtt
     7561 cccggctggt ttcagcctgt gctggtgctg tgcgacgccc agggacgcga gaaggcatac
     7621 aacgacgatt accgcttcca tccggaccct gtcctgtact gcgagctttc cgcgaacggc
     7681 gagtacgtgt tggcgatcca tgacgcgatt ttccgcggac gcgaggattt cgtctaccgg
     7741 atgaccatcg gcgagttgcc gtttgtgacg agcgtgttcc cgctcggcgg gcaggccggt
     7801 gtcgatgcgg tgatcgaggt caagggtttc catctcgcgg agacacgtct gacccccgac
     7861 ctgcggacgc ggcttccggg tctgcacacg ctcacggttg cgggatcggg cggattgcgt
     7921 tccaatccct ttgtttttga gatcagcacg ttgcccgacg gaatggagca cgaacccaac
     7981 aatacgctta aacgggcgca accggtgacc ctgccgatcg tgatgaatgg gacgatcggt
     8041 atgccgggcg atttggatgt cttcacgttc cgcggtcggg ccggacagga ggtcgtggcg
     8101 gaggtcaggg cgcggcgact cggttcgccc cttgacgcct ggctgaagat catggacgat
     8161 caggggcgga ctttggcgtg gaatgacgat caggaagatc cgggttccgg actggacacg
     8221 caccatgccg acgcgtatgt gcgcgtcacc cttcccgcgg acggcgccta tgcggtgcag
     8281 ctccgggata cgcagcatca gggcggcgag gccttcggct atcgcttgcg gctcagtgag
     8341 ccgcaaccgg attttgacct gcgcgtggtg ccgtcgcggg tggttgtgcg gcgcggcggg
     8401 gagaccccgg tcacggctca cgccctccgc aaagacggat tcgccggtcc catcacgttc
     8461 cgcatccggg agcctgccgc gggatttgag gtgcggggcg cgaccctgac gggcacgcaa
     8521 accgtggcgc gcctcatgat ccgggcgcgt caaccatcgg attccggggt gccggtgccg
     8581 atcgtgatcc aaggcgtgtc aggtgagggg gaaggggcga tcatccgcga cgccgtggca
     8641 gccgaagatt gcatgcaggc ctttctctgg cgccatctgg tgcctgcgca ggaatttctg
     8701 gtcctgatga ttccgggccc gccgagaccg tcaggcaagc cggggtttcc ggcagcttca
     8761 cgtcggcggg aaaaaccaga atgaaattta tgtccgtgac tcgggaatgc gaatcacgcc
     8821 atgcgccgga cttgttccct gtttgagaat accctatact gtcggactga aacacgggga
     8881 cgaaatgagt gcgcgaaaac tgacggcgaa aataaccggc gtgggcaagg cgcttcccga
     8941 agggcggctg accaatgcag atctcgagaa acgggtggac accaatgacg cctggatcac
     9001 ccagcgtgtc ggtatccgcg agcggcggat cgcggccgag ggggagcaca cctctgactt
     9061 cgcgatccgg gcggcgcgtg acgcgttggc cctctccggc ctgaaacctg aacagatcga
     9121 cctgatcctc gtagggacct cgacgcccga tatgatctac ccctccgtgg cctgccaggt
     9181 gcaacaggcg ctcggcgcgt ccaacgccgg ggtgctggat atcagcgcgg cctgcacggg
     9241 attcatctat ggcgccagtc tggcctgggg tatgatccag tccggggccc tgcgccatgt
     9301 gctggtgatc ggtgcggaga aaaacagcgc gatcgtggat tggtccgacc gcgacacctg
     9361 catcctcttc ggggacggcg cgggcgcggc aatcttcagc gagcaccgct cgaacacgga
     9421 cggcgtgctc tccgccaaac tgggcggtga cggttcgttc ggcggactgc tccatttgcc
     9481 gaacagcggg tgccggtctt ttcccgaaga gaacggacgc ttcatccgga tgcagggcaa
     9541 cgaggttttc aagcaggcgg tgcgcaatat gtacgagtgc gcggtctgtg tgcttgatca
     9601 ggcaggactt tcgagtcagg aggtgtcgct gctgatcccg catcaggcga atgtgcggat
     9661 catcgacgcg gtggccaaac ggttggggct gcccaaggag aaggtgttcc gcaatatcgc
     9721 ggagtacggc aacacctcgg ccgccacgat cccgatcgcg ctttgcgagg cccttgaaca
     9781 ggggcgcctt aagaagggcg acgtgctggt gatcgacgct ttcggcgcag gcctgacgtg
     9841 gggcgccatg gcgatccggt gggcgtgagg cgcgtcacac gacggcgttt gtttcaaaag
     9901 ccatggcacc cgtgggacac gcggcggcac aggcctgcag cacgctgtcc ggaagatcgt
     9961 cccacacgcg gccgagtggt gggccgatcc gcgcctcgaa gcctcgtccg tgaaaagcgg
    10021 gccccaactc ccctgcgcca cactgccgcg taatccgcac gcagagtccg cacagtatgc
    10081 agcgcgacgc atcgaagcgg aatccgttgc ggcccgtctc gcggatcacc ggtcgcggcg
    10141 cgtggtcgaa cgcccgcggg ttcgcgccgt attcggcgca caactcgcgc aaccggcact
    10201 tcgtcggctt caggcacccg caccgcatgc agcgcccggc ctcgaacgcg gcagcttcgg
    10261 gtgtgggttc gtgcgtctcc ggcggcaggc ggcgcggtcc ggtctccgga gtcagcgcgg
    10321 ctagggctgc cacgttcaaa tcgggcatcc gtgatcggaa gcgggggcgc gcctgagtgc
    10381 cgcccggcac gcggctgcac gcctccccgg cggtttcgcc ggccagccag cggtggagtg
    10441 cgcagatcgc gaccgcgccg tccagcaggg cacggcggca cgctttttcg catggtgcgg
    10501 ctccgccgca cgcaaaggga tgggcgcggg ccagatcccg cgcgctctgc caatcacccg
    10561 accggatccg ggccatcagc gtttggacgg gtagccccga ggggcaggcc atccggcagg
    10621 gcgcctcaca atccgccgga tggttgctca gcaggagttc cagcgcggtg cgccggaagt
    10681 tgcggaccgc ttcggaatcg gtaacgatgg acatggcggc gtccgcgcgc gtcgcacagg
    10741 cgggtaacag ctttccgccc gcagcggtat cctccactgt gcagaggccg cacccgcccg
    10801 cgggcacgac tccgtcggca tgacacaaca ccgggatgcg gataccgatc ccctgtgccg
    10861 cctcatacac cgtcatcccc ggtttcagcg tcacggggcg gccgtcgatg gtcatgcccg
    10921 cggcctcatt tgcgtgaact gcggcttgac gcttcgggtt gatagagcag cttttcgatc
    10981 acgaaacggc ggtggcccgc aggcaccatc cattcgatgg tatcgccctc ggcgtaaccc
    11041 aaaatggcgg taccgaccgg cgacagcaca gagagttttc ccgcgtcgac attcgcctcg
    11101 gacggcatca ccaaagtcac ttccatcacg gtctgcgtct tcacctcgcg cagacggacc
    11161 cgcgaatgga gggtcaccac attcggcggc acctgctcgg gctcgacaac cagcgcgcgc
    11221 tgcaactcgt tctgcagcga tttcagatcc ccctgatcgc ggtcatggga aagatgggcc
    11281 acattcacca attcttcaag tttatggcga tccatctcgc taatgtaaag agttcgcttc
    11341 gtcatatcgg gtcacctcct gaaaaacaaa gagggctaag atgcggtcag catagcatcg
    11401 gataggctgg aaacacaagc aaaagagggg ataaaacggc gggcccgttt gccccgcctg
    11461 cggtgataga caggcgtgcc gaaatatgcg ataatttggc gcgagaggtg ttccgtgttg
    11521 aaagtgttga gcgcaagtta ttgatgcgtc cggttgggtc gcagcgggtt gcgagattcg
    11581 aggggttgcc aaccgtccca atccatttac agaacaggtt acgcgtcccg cccggggttc
    11641 cgggcgaaaa cgaaggggta accggaacgt tcaggttatc gggagggacg cctccccgag
    11701 gcgtccgagg cggtttcggg gaaaccgccc taccggttgt aacacgggtt tggtgtgaca
    11761 aaaagatcag gctatagaca aggaaaggga agcgatgatt cgaaataaac aaattcttag
    11821 cctaatgttg ttgacggcgt ttctggcggt atccgcccgc ggcgtctaca tggtgcatga
    11881 gttctacctg ccgctgcccg aggcgcaggt gctgaaggcc ttcaacactc tcgacggcct
    11941 tgggcaagtc aaggcgccca tcgacagcgt aacctcgatc gtcgtcaccg gcccggatac
    12001 catcatccat tatgaccact gggaggatgg ctatgaggtt gatatcaaca acccggccca
    12061 gccgacgact cgcatctggg gcgacggcaa cgacgccaac ggcatcccgc ccggctacgc
    12121 ctccgatccc atggggctgc cgcagggtgc cgtcatctcg ttgcgcaaca acgtcaacct
    12181 gccgcgcaac ccttcgcaga tcctttacga tggtcgtgac cgcatcacgg ccagcaaggc
    12241 gctcgccgtt tcgcgcgccc tctggcccgt ctcggtcggc acggtgctgt ccgaggcggt
    12301 cgaagtgacg gccacgattg atcacggcac gcactacatc gcgccggtgg gcgaggatgt
    12361 cgatgccttc gagatgttcg agcaggtcgg cctcgccgtc atggccgatc aggacaacac
    12421 cgtcgtgacg atcgacaagg acggccccgg cggcgcggcg ccggtcaccg tcaccctcaa
    12481 tcagggcgag agccactacg tcagcggcgg cgtcctgaag ggcgctgagg tcacggccac
    12541 caagcccgtc caggttcatc tgctgacggg cgacaccggc gcgacctatg agacgcgctg
    12601 gtacacgctc ttccccgtcg aggactggtt cagccgctac gtgacgccga tcggcagctc
    12661 cgccaacggg caggcgtgct ttgtcttcct ctataatccc ggtcccgaaa ccatcaccat
    12721 cgaagccgcc tcgcgcatcg gcacggagag catcacggtg ccggcgggcg aggtctaccg
    12781 ctggcaggtg ccgcagaact cgggcgcgat gttcagcagc gccggggatg agcccttcag
    12841 cgccatcgct gccgcgggcg ctcaccccaa cgccaatgcc gcctacgact ggggcttctc
    12901 gctggtgccc caggacgcgc tctcctccac agccgtcgtc ggctgggggc cgggcagcag
    12961 cgacatgacg cgcaacggca gcccggtctt tgtctcgccc gcgaccgcca cgcgcatcta
    13021 tgtggactac aacggcgacg gccaagggcc gctgaccgac cccaacggcg ggaagtacga
    13081 caaacactac gatctggccg cgctccagtc gcagaccctc ttcgatccgg atggcgacca
    13141 gaccgccatg cgcatctaca cgatcgacgg cgcgctcttc tccgcgtcgt ggggccagga
    13201 tccggacacg gcccgccccg gaaacccctt cctcgacatg ggcagcaccg tcttgccgct
    13261 ccccgaaccg aagctggtca agaccgcccg catcgtgaac gacgtcgatc ccgccggccc
    13321 gagtctcggc gacacaatcg aataccgcat tgtgatcgac aacaaggggc tgctgccgct
    13381 gggcaacacg ctggtgctgg acaccctgcc atcgcaactc agttacgtga ccaactccac
    13441 cacgctggac ggcgagccgc tgccggacga cacggtcggc agcacgctct tcccgctgga
    13501 tgaggatggg tacaccatcc cggttatcct gcgcgctggc accagtatct tcacctacca
    13561 gacgaccatc gtcggcacgg gcagcatcgt caaccgtagc gctgtgccgc agtatgaagt
    13621 ctatgacgag accggggccg atgttccgcc gcccgccggc agccatccct gcacggtgga
    13681 gttcgtcgat gccggcggca atccggtgac cgtctatcgc gaaggcgccc ccatctacct
    13741 ct
//
LOCUS       DAOSLE010000073        13431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4681_length_13431_cov_7.200508, whole genome shotgun sequence.
ACCESSION   DAOSLE010000073 DAOSLE010000000
VERSION     DAOSLE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4681_length_13431_cov_7.200508"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..528
                     /locus_tag="PK576_06390"
     CDS             118..528
                     /locus_tag="PK576_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE61259.1"
                     /translation="MALRRLGRRKPSKVINNIDMTSLIDLTFLLLITFIITMPAIEQG
                     ISIRLPQGKADELPVKKSNVVTLDVEGKAYLNNRVISLEDLERTLGNMAAEDPEVAVL
                     VRGDERLEYGKIMRVMKILYKVKITRMALVTLSE"
     gene            536..1384
                     /locus_tag="PK576_06395"
     CDS             536..1384
                     /locus_tag="PK576_06395"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOE61260.1"
                     /translation="MRKGPLNSYSLGWSLALHGVLLVAAIVLPLLSFLRPKPLQIPID
                     FTVVLEENLVEPNVPDTPPAPPTEPEAAPEPDPAPPKPAPLPDPPKDAVVVEKKKEPE
                     KKKEPEKKKEPEKKKEPEQKKPAPFKKGKRIEAPKQPQQPRFEDLYKPYDPSKPQSRK
                     PKTDKMLTRAEIEKALRDGARPGSRNVIPQDEIARCVTLVRRAMYEAWEQPGSGDAGS
                     RPALLDIRLDQAGRVVSYRIRQSSGSPYFDQTVLKAAANAAPIRGLTPAFLKRYETLT
                     VEFKLE"
     gene            complement(1568..3043)
                     /locus_tag="PK576_06400"
     CDS             complement(1568..3043)
                     /locus_tag="PK576_06400"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE61261.1"
                     /translation="MHLPPALREKLAALPDKPGCYLMRDRTGTIIYVGKAVSLRRRVQ
                     SYFRPSTLRAAPPKLRGMLRSVADLDILTVRNEAEALLTESQLIKQYRPRFNVVLRDD
                     KRYLALRADPREPVPRLATCRIVRADSAMYFGPFPSATVVRTVLDFTEKRYGLRKCTP
                     LEPDAETYRHCINDIVRFCSAPCIGRISREAYRKRFDEACAFLRGERPAVIEEVRTRM
                     DEASQKRDFEKAASLRDTWLALREMVRRRAITVAAPERHAEDARDGILDLQRRLGLPH
                     VPARIEGFDISNLFGTHSVASMVVAVDGLPERRQYRRFRIRTVEGADDPRAIAEVIRR
                     HYARMRGEHRPLPGLVLIDGGIPQLRAAREVLDTLGLGRVPTAGLAKRMEEIVLDNGA
                     PPLRLPYDSPALRVLIRLRDEAHRFALDYHRRLRNRLIRESALDEIPGIGPAKKAALL
                     RRFGSVYRLARASAAEVADVPGISPGLAEAILRAVDTRKTP"
     gene            3179..4840
                     /locus_tag="PK576_06405"
     CDS             3179..4840
                     /locus_tag="PK576_06405"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOE61262.1"
                     /translation="MKRKTYVIGHRNPDTDSIVSAIAYAELKRQKGWTECFAARAGKL
                     TPQTEYILGRFNIPAPEFVPDLVPKVGYFLTGGAVTVGESEAFWDALETMEREKVSIL
                     PVVDAQGRYRAVLHHNAFASNITRKINPHRKAIIPTSMELLQKTLQAQPIVTFAAEEV
                     VRSRILVAGSSFESFREHLENEIPANALVLTGDREDVQRFSIESGARAVIITNGMPLA
                     KGLRELAEARRVTVMISPYDTSSTAFLILYSTPVMTVADEDAMPIAANSYVRTVREQL
                     AAAPSHCLPVVDDAGIVVGIFSESDLIAEPNIDIIMVDHNEMGQAVEGLENYRILEII
                     DHHRLGNFSTRYPITFINRVVGATSTIVAGMYIEQKAVLTPGVAALLLAGILTDTLML
                     RSATTTETDREMAEYLAGLVNLDIESFGHEIFSNARKVADLPVVDILGMDSKAYAACG
                     QRFAVSQVEVNAADDLLVRKAELLAALRQRRTGEGLFFTALMITDVTTLSSILLVEGD
                     ETFIKKIAFPRLEPGVFRCRDILSRKKQLIPLLIEIMETVLGVNA"
     gene            complement(4830..8054)
                     /locus_tag="PK576_06410"
     CDS             complement(4830..8054)
                     /locus_tag="PK576_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE61263.1"
                     /translation="MIPFDLLTTTLAPGTRFLIEANAGTGKTFQIQHLYLRLILEQNL
                     EPSRILVVTFTEAATAELRDRIRQCLATALAKLGEVEPENPPSLIDAILKQAAAQRTP
                     DALRVSLQLALAAFDEAAIFTIHGFCKRTIDHHAFESQVMFDCELIPSASELLGDLVA
                     DFFRRERHAKAEPFVSLHDLLNHAHLVMSHLGEARIEADGVTDPRLDVFARLRDHLHD
                     PEDGLTARMRRRQQMGYDDLLRILRDALAAGPDCPLAHVLRATYAAALIDEFQDTDPV
                     QYEIFKTVFAHPDSRLFLIGDPKQAIYGFRGGDLYTYLQARDEVAPSARFTLTRNFRS
                     ARELIEAVNSIFAPPGAFGEQQLGHPPSESGREPERTLLRDGHRDPTPFELVRFTGDH
                     TGGAVASSSLETPITRDCAARIATLLTDGRTMFQKPDQTLIPVTPGDIAVLTVTNSQA
                     EALQQRLRLHGVPAVIYKAGNIYASADAGNLCRLLGAMAEPNRVPRIKAALLTPHGGM
                     TVRDLLAAEKDPDAAADLERRIGDFAECGRLWREKGVVPALTLLLARLNSLERLANDP
                     ACERRLTNLRHLTELLHQTERAARLAPDALLRELKRQIADPDTDDETCEQRMESDRRA
                     VTLMTIHKSKGLQFPIVFVPYLITHDIRKGMEKGWAVHAPDAQGVMRLVLPVGDAARR
                     RFEEQRLHETLAEHLRLLYVAVTRAENRCVLMTGTAITYKKTPIRSAVAHLTRLAEPM
                     STGGTPFSLEEVTTETLDRPHAEPRHTPPDTHGDLVPPASPPLPPDDWAVLSYSGMTL
                     HDAPPPHLKPDAGTDEVADPLLSAPDRDRAGDVLPGGKTTGLCLHAILDQLDYTHVTP
                     AWQPAPDDLALIDRHAAFHGLYTSDSPLAAARRARIVDLLAQTLTRALPTAPADPGAN
                     APMSGGIPLSAVPFADTRREWEFFFRVPRAIDLTRFQELGLSFKPDSGTRRGFMTGSI
                     DLVFSCEGRTGFVDWKTDMLPDYTPATLAAAMTERNYLFQAILYAVALHTHLAQCLGA
                     AYDYERHFGGGHYVFIRGVDARNGLFPFRPPLAELEAWSRLLTH"
     gene            complement(8051..11437)
                     /gene="recC"
                     /locus_tag="PK576_06415"
     CDS             complement(8051..11437)
                     /gene="recC"
                     /locus_tag="PK576_06415"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOE61264.1"
                     /translation="MSFTLYHGNRLDQLAAALADLILQPPAGGAVRDPLRQETIVVPN
                     QGLARWLALEIAARRKICPPLDLPYPGAFLYRDLFDPMMGRTSRAVQPDLPFDPATME
                     WRLFALLEQLEPETAFARVHAFTRCDPLRRYQLAGRLASLYDRYMTYRPDLLAAWEQG
                     DEPLTGEDAAWQARLWQELIALDRGSVRHFSGLYRRFIELTRHPDRQPDFLAPLLARR
                     RIAYFGVSSLPPAHLDILIRLARFDDFEIHFFTPNPCCEEWSDARSLKTQIRDRLTLV
                     QTIGPQLAPAYEIRANPLLGSLGRSGREFFSLLLAYDEIHDERLFVEPDAAEGTVLGT
                     LQRGILRNTPLEPRQPVAPSDRSLVIHACHAPLREVEALHGHLLALFRDEPGLAPREI
                     SVYTPDIETFAPYIEAVFGKVPPGTPGHIPYTIADKSLLHTVDECRAWLALLTAVTGR
                     FKASEVLGLLQHAALRQRFNFVETDMPCLGSLLRAAHLSWGLDAAFRARQGASPAYAN
                     TWRFALDRLLFGAAMQDPTDEAPPLNLTEGKCIPLTEAEGHASLIGRLASFLDALAEL
                     HEFCLSRPTRPCGAWHAYLNAALERFFPTDERASYGVLLLRQTLDQFARYIEHAGCER
                     LAVPFSVIRAWLTAQLDGTPGSERFVTGRVTFSRFQPMRNVPARVVCLLGLSDKAFPR
                     NAPQLSFDLMDRRENRCIGDRRPRDEDRYAFLETLLAARERLIITYTGQSNKDNKPLP
                     PSVLVSELLDAIDAAFTFPSRDGVPQTASTALIVRHPLHPFSPAYFTRDKPDPRLRAF
                     DATAFRIACRLAGATGDASPQPEAQVVFPSAESAATDAPLQLEELIAFFLSPCKAFYT
                     RRLNAALTERDDELPDDLEALEPDTLVQYQIRQELTAAMLHGDGELAINRLRDRWQTD
                     GRLPVDADRHMAEARDRIDVVIQKVNALDLGEPLAPQPFDLDLPNGFRLQGTLNALRA
                     GNAQLFTRPAKEKPKDIVTAWITHLAACAVKPGVTTWGVFEGTKTIVTRYPPLESDAA
                     RRILAALAAHHAEGMTRPLCFDPAIGHKLVEGKPLTETDWTGSSFPSDPGVDIYLQHA
                     FGATLPEAGSEPLRTLERVARTLFGEMRKTPVKIQRGGRS"
     gene            11760..13430
                     /locus_tag="PK576_06420"
     CDS             11760..13430
                     /locus_tag="PK576_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61265.1"
                     /translation="MKPVVVMTGVAVGWIVFSAAAETNYWIATSGTGNWNEPANWSLD
                     RVPGAEDTVIITNAATTGNGYTVEIDQAPVNVFRVTCSAPEGQMLTLNVRTNLTVNNN
                     SSSDADWSFGPKGNVAINIHSNAELDAYCQILGAGESNNYTVLPGGTLRYGRTPSKRF
                     RVYGSLTVQGSVIAGNRAGELIYANPLILDGGYLFAKALSSPPELVTNNASLSYSLMR
                     IAGMTVTMRDGTVTNRADDALHIGNAASGNASAFLNLSGGLWRQEGPAYICERPNGYL
                     DLSGTAQFVSTNNVWVGSNNTGTQPFLPGYSGFVTVHSGNFCVTNSDGSARLYLGNIT
                     PGYLTCAGGTSSVDRLVLSNRGNVLGADAVGRITMAGGRLDILQSLSATNGSYSVLAC
                     STGTLNVANADVANDSALVIGDGKGEATLGLLSGTHRFADGLVITNNATLSAGGVGNR
                     ASAEVTGNLALAEGATLHCDFQDNAGDSLSLAGKLTLPTQAAVTLTSLDGTLPQHVVL
                     VAAEALDGAVDLSGWTVASIGEIGYRVAVEGASLVLLKKPKGMVISVL"
BASE COUNT         2513 a         3747 c         4712 g         2459 t
ORIGIN      
        1 cgcgatctgc tacaacaatc tggcggcgaa ggtgcggcag atcacgaccg acatggacgg
       61 gttcgcggat gaactgatgg gccgcatcgc ctgcgagttc caagggagga ccgactaatg
      121 gccctgcgtc gacttgggcg caggaaaccg tcaaaggtca tcaacaacat cgacatgacg
      181 tcgctgatcg acctgacctt cctgctgctg atcacgttca tcatcaccat gccggccatt
      241 gaacagggga tttccatccg cctgcctcag gggaaagcgg acgagttgcc ggtcaagaaa
      301 tccaatgtcg tgactctgga cgtggaaggc aaggcgtacc tcaacaaccg cgtgatctcg
      361 ctggaggatc tcgaacggac attggggaac atggcggcag aggatccgga agtggcggtg
      421 ttggtgaggg gggatgaacg tctggaatac ggaaagatca tgcgggtgat gaagatcctg
      481 tataaggtca agatcacgcg catggcattg gtgaccctgt cggagtgagg caagcgtgag
      541 gaagggtccg ttaaacagct attcgctcgg ctggtcgctg gcgctgcacg gggtgttgct
      601 cgtggcagcc atcgtgttgc ccttgctgtc gtttttgcgg ccgaaaccgc tacagattcc
      661 gatcgatttc acggtggtgc tggaggagaa tctcgtggaa ccgaatgtgc cggacacgcc
      721 gcccgctcct ccgacggagc cggaggccgc gccggaaccg gatcccgcgc cgccgaaacc
      781 cgcgccgctg cccgatccgc cgaaagatgc ggtggtggtc gagaagaaga aagagccgga
      841 aaaaaagaaa gagcccgaga agaagaaaga gccggaaaaa aagaaagagc cggagcagaa
      901 gaaacccgcg ccgttcaaaa aaggcaaacg catcgaagcg cctaagcaac ctcagcaacc
      961 caggtttgaa gatctttaca agccgtatga cccgagtaag ccgcagagta ggaaaccgaa
     1021 gaccgacaaa atgttgaccc gcgcagagat cgagaaggcg ctacgtgacg gcgcgcggcc
     1081 gggcagtcgg aatgtcattc cgcaagacga gatcgcgcgt tgcgtcacgc tggtacggcg
     1141 cgcgatgtac gaagcgtggg agcagcccgg ctcgggcgac gctggctcac gtcccgcgct
     1201 gctggacatt cgtctggatc aggcagggcg tgtggtgagc taccgcatcc ggcaatcttc
     1261 aggcagcccg tatttcgatc agacggtact caaggccgcc gccaacgccg cgccgatccg
     1321 ggggctgacg ccggcttttc tcaagcggta cgagacgctg acggtggaat tcaaactgga
     1381 gtaaaaaacg gcggcatcat tgcgtgtgcc gctgtggcat gtgccgcttc ggttcccgcg
     1441 cctacccgcg ttcgcgacgc ttgccgccgc tgcggaatcc ccgttcttcg cggggtttgg
     1501 gctgcgacgc gttcacgcgc aggggacgtc cttcacattc gtaaccgacg tgcctgcgcg
     1561 gtaacgttca cggcgttttc cgcgtgtcca cggcacggag gatggcttcc gccaagcccg
     1621 gcgagatgcc gggaacgtcg gccacctcgg cagccgaagc gcgggcgaga cggtaaaccg
     1681 agccgaaccg gcgcagcagg gcagcttttt tggcggggcc gatgccggga atctcatcca
     1741 gcgcggattc gcggatgagc cggttgcgca ggcggcggtg atagtcgagg gcgaaacggt
     1801 gggcctcgtc acgcaaacgg atgagcacgc gcagggccgg ggaatcatac ggtagccgga
     1861 gcgggggcgc accgttgtcg aggacgatct cttccattct tttagccagt ccggccgtgg
     1921 gcacgcgacc gagcccgaga gtatcgagaa cctcccgcgc ggcgcggagt tgggggatgc
     1981 cgccgtcgat gaggaccagc ccgggcaggg ggcggtgttc cccgcgcatg cgggcatagt
     2041 ggcggcggat gacttcggct atggctcgcg gatcgtccgc accctccacg gtgcggatgc
     2101 ggaagcgccg gtactgacgc cgctcgggca ggccgtccac tgccaccacc atgctggcga
     2161 ccgaatgcgt gccgaacagg ttagagatgt cgaagccttc gatgcgtgcg ggcacgtgcg
     2221 gcaggccgag tcggcgctgc aagtccagaa tgccgtcacg ggcatcctcg gcgtgccgtt
     2281 cgggcgcggc gaccgtgatc gcgcgccgac ggaccatctc gcgcagtgcc aaccatgtgt
     2341 cgcgcagcga agcggccttt tcaaaatcgc gtttttgcga ggcttcgtcc attctggtcc
     2401 ggacctcctc aatcacggcg ggccgttcgc cgcgcaggaa tgcgcaggcc tcatcgaaac
     2461 gcttccggta cgcttcgcgg gagatgcgcc cgatacaggg ggcggagcag aaacgcacga
     2521 tatcgttgat gcagtggcgg tacgtttcgg catccggttc caacggggta catttgcgga
     2581 gaccgtaacg tttttccgtg aaatcgagca ccgtgcggac gacggtggca gaaggaaagg
     2641 ggccgaagta cattgcggag tcagcccgca cgatccggca ggtggcgagc cgggggaccg
     2701 gctcacgcgg gtctgcgcgc aacgcgagat accgtttgtc gtcacgcagc acgacattga
     2761 aacggggacg gtactgtttg atgagctggc tttcggtgag gagcgcttcg gcctcgttgc
     2821 gtacggtgag gatgtccaga tccgcgacac tgcggagcat gccgcgcagt ttggggggcg
     2881 cggcgcgtag ggtggagggg cggaaataac tctgcacgcg gcggcgcagc gagaccgctt
     2941 tgcccacata gatgatggtg cccgtgcggt cgcgcatcaa atagcagccg ggtttgtccg
     3001 gcagggccgc gagtttttct cgaagtgcgg gggggagatg cataaccctc caaggtgaca
     3061 caaaaaccgg gttcacgatg tcgatggcgt tagtttggca tgcggagaaa cctccggcaa
     3121 ggggaattcg gattgcctct ggcttttctg gaagcgatag cctatgattc tgcctcgcat
     3181 gaagagaaaa acctatgtga tcggacaccg taatcctgac accgactcaa tcgtctcggc
     3241 gatcgcctac gcggagttga aacgccagaa gggctggacc gagtgctttg ccgcacgcgc
     3301 gggcaaactg acgccgcaaa cggagtacat cctcggccgg ttcaacatcc cggctccgga
     3361 gttcgtgccg gatctggtgc ccaaggtggg gtattttctc acgggcggcg cggtgacggt
     3421 cggtgaatcg gaggccttct gggatgcctt ggagacgatg gagcgggaga aggtgagcat
     3481 tctgccggtt gtggatgcgc aaggccgtta ccgggctgtg cttcaccaca acgcctttgc
     3541 cagcaacatc accagaaaga tcaatcccca ccgcaaggcg atcatcccca ccagcatgga
     3601 actgctgcag aagacgcttc aggcacagcc gatcgtgact ttcgccgcag aggaggtggt
     3661 gcggtcgcgt attctggtgg cgggatcgtc attcgaatcg tttcgggagc atctggaaaa
     3721 tgaaatcccg gccaacgcgc tggtgctcac cggtgaccgc gaagacgtgc agcgcttcag
     3781 tatcgagagc ggtgcgcgtg ccgtgatcat caccaacggc atgccgctgg ccaaggggtt
     3841 gcgcgagttg gctgaagccc gccgcgttac ggtcatgatc tcgccgtacg acacttcctc
     3901 gaccgccttt ctgatcctct attccacgcc ggtgatgacc gtggctgacg aggacgccat
     3961 gccgatcgcg gccaacagct atgtgcgcac cgtgcgcgaa cagctcgccg ccgcgccctc
     4021 gcattgcctg ccggtggtcg acgatgcggg catcgtggtc ggcatcttct ccgagagcga
     4081 cttgatcgcc gaaccgaaca tcgacatcat tatggtcgac cacaatgaga tggggcaggc
     4141 ggtcgagggt ctcgaaaact accgcatcct cgagatcatc gaccatcacc gcctgggcaa
     4201 cttctccacc cgctatccga tcacgttcat caaccgcgtg gttggcgcga cctcgaccat
     4261 cgtcgcgggg atgtacatag agcagaaggc ggtgttgacc cccggcgtgg cggcgttgct
     4321 gttggcgggg atcctgaccg acaccctgat gctgcggtcg gcgaccacca ccgagacgga
     4381 tcgcgagatg gctgaatatc tggctggatt ggtgaatctg gacatcgagt ctttcggaca
     4441 tgagattttc agcaacgccc gtaaagtggc ggatctgccg gttgtggaca tcctcggcat
     4501 ggactccaaa gcgtacgcgg cgtgcggaca gcgttttgcc gtcagtcagg tggaggtcaa
     4561 cgcggccgac gatctgctgg tgcgcaaagc cgaactgctg gcggcgttgc ggcagcgccg
     4621 cacgggggag gggcttttct ttacggcgct gatgatcacc gatgtcacga cgctcagctc
     4681 gatcctgctg gtcgagggcg acgaaacctt tatcaaaaag atcgcctttc cgcgtctgga
     4741 acccggggtc ttccgttgcc gcgatatcct ctcgcgtaaa aaacagctta ttccgctcct
     4801 gattgaaatc atggagacgg tcctcggcgt caatgcgtaa gcaggcggga ccacgcttcg
     4861 agttcagcga gcgggggacg gaaggggaag agtccgttgc gggcatccac cccgcgtata
     4921 aagacgtagt ggccgccgcc gaaatggcgc tcgtagtcgt aggccgcacc cagacactgc
     4981 gcgagatgcg tgtggagggc cacggcgtag aggatcgcct gaaagagata gttgcgctcg
     5041 gtcatggcgg cggctagcgt cgcgggcgtg tagtcgggga gcatgtcggt cttccagtcc
     5101 acgaaaccag tgcggccttc gcaggagaaa accagatcga tcgatccggt catgaagccc
     5161 cgccgcgtgc cgctgtccgg tttgaaggac aggcccaact cctgaaatcg cgtgaggtcg
     5221 atcgcgcgcg gcacgcggaa aaagaactcc cattcgcgcc gcgtgtcggc aaagggaacc
     5281 gcggagagcg ggatgccgcc gctcattggg gcatttgcgc cgggatcggc gggagcggtc
     5341 ggcagggcgc gggtcagggt ttgggcgagg aggtccacga tacgggcgcg gcgtgcggcc
     5401 gcgagcgggg agtcggatgt gtagagtccg tggaaggcgg cgtggcggtc gatcagcgcg
     5461 aggtcgtccg gtgcgggttg ccacgccggg gtgacgtgcg tgtagtcgag ttgatccagg
     5521 atcgcgtgca gacagaggcc ggtggttttg ccgccgggca aaacatcccc ggcacggtcc
     5581 cggtccggtg cggacaagag ggggtcagcg acctcatcgg tgccagcgtc cggtttgagg
     5641 tgcggcggcg gggcgtcgtg gagggtcatg ccggagtaac tgagtacggc ccagtcatcg
     5701 ggcggcagcg gcgggcttgc gggcgggaca aggtcgccgt gcgtgtccgg cggcgtgtgg
     5761 cgcggctcgg cgtgcggacg gtcgagggtt tcggttgtga cctcttccaa cgaaaacggc
     5821 gtgccgccgg ttgacatggg ttccgctagg cgggtcagat gcgccaccgc gcttcggatc
     5881 ggggttttct tgtaggtgat cgcggtgccg gtcatcagaa cacagcggtt ttcggcgcgg
     5941 gtcacggcga cgtagagcag gcgcaggtgt tcggcgaggg tctcgtgcag ccgctgctcc
     6001 tcgaaccggc ggcgggccgc gtcgccgacc ggcaggacga ggcgcatgac accctgcgcg
     6061 tccggtgcgt ggacggccca ccccttctcc atgcctttgc ggatgtcgtg cgtgatcagg
     6121 tagggtacga agacgatcgg gaattgtaac cccttgctct tgtggatggt catgagcgtc
     6181 acggcgcggc ggtcgctctc catgcgctgc tcgcaggttt cgtcgtcggt gtcgggatcc
     6241 gcgatctggc gtttcagctc gcgcaacagc gcatccggcg cgagacgggc ggcgcgttcg
     6301 gtctgatgca gcaattcggt gaggtgtcgc aggttggtga ggcggcgctc gcaagcggga
     6361 tcattggcga gtcgttcgag gctgttgaga cgggcgagca aaagcgtgag cgcgggaacg
     6421 acaccttttt ctctccagag gcggccgcat tcggcaaaat caccgatgcg acgctccaag
     6481 tcggcggcgg cgtccgggtc cttctccgcc gcgagcagat cgcggacggt catgccgccg
     6541 tgtggcgtca gcagggcggc cttgatgcgc gggacgcggt tgggttcagc catggcgccg
     6601 agcagacggc agaggttgcc cgcgtctgcg gaggcgtaaa tgttgccggc tttgtagatc
     6661 acagccggga cgccgtgaag gcgcagccgc tgctgaagcg cttcggcttg gctgttggtg
     6721 acagtcagaa cggcgatgtc gcctggcgtg accgggatca acgtctgatc cggcttttgg
     6781 aacatggtgc gaccgtccgt cagcaacgtg gcgatgcgtg cggcgcagtc gcgggtgatg
     6841 ggcgtttcca gcgaggagga ggcaaccgcg ccgcccgtgt ggtccccggt gaagcggacg
     6901 agctcgaacg gcgtcggatc gcggtggccg tcgcgcagca gggttcgctc gggttcgcga
     6961 ccgctctccg aaggcgggtg gccgagctgt tgttcgccaa aggcgcccgg cggcgcaaag
     7021 atactgttga cggcctcgat cagttcgcgg gcggaacgga agttgcgcgt cagggtgaag
     7081 cgcgccgacg gcgcgacctc gtcgcgagcc tgaagatagg tgtagaggtc tccgccgcgg
     7141 aaaccgtaga tcgcctgttt ggggtcgcca atcagaaaga gtcgggagtc ggggtgggcg
     7201 aaaaccgtct tgaagatttc gtactgcacg gggtcggtat cttgaaactc atcgatcagc
     7261 gcggccgcgt aggtggcgcg tagcacgtgc gccagcggac aatccgggcc agcggccagc
     7321 gcgtcgcgca ggatgcgcag cagatcgtcg tatcccatct gctgtcggcg gcgcatgcgc
     7381 gcggtcagac cgtcctcagg gtcgtgcagg tgatcgcgca agcgggcgaa aacatcgagt
     7441 cggggatcgg tcacgccgtc ggcctcgatc cgtgcttccc cgaggtggct catgacaagg
     7501 tgcgcgtggt tgagaagatc gtgcagtgaa acgaatggct cggctttggc gtgccgttcg
     7561 cggcggaaga agtcggcgac cagatcgccg agcagctccg aagcggaggg gatgagttcg
     7621 caatcgaaca tcacctgact ttcaaaggca tggtggtcga tcgtacgttt gcaaaaccca
     7681 tggatggtga agatcgcggc ctcgtcaaac gcggccagag cgagttgcag gctcacgcgc
     7741 agcgcatcgg gtgtgcgctg cgccgcggcc tgcttaagaa tcgcgtcaat cagcgatggc
     7801 gggttttccg gctctacctc tccgagcttc gccagcgccg ttgcgaggca ttggcggatg
     7861 cggtcgcgca gttcagcggt cgcggcttcg gtaaaggtga cgaccaggat gcgcgacggt
     7921 tcgagatttt gctcgaggat caggcgcagg taaaggtgtt ggatttggaa ggtttttccg
     7981 gtgccagcgt tggcttcgat caggaagcgc gtaccggggg cgagcgtggt ggtcaagagg
     8041 tcgaacggga tcatgagcgc cctccccgct ggatttttac gggtgttttc cgcatttcac
     8101 caaagagtgt gcgggccacc cgttccagtg tgcgcagggg ttccgaaccg gcttcgggga
     8161 gcgtcgcgcc gaaggcgtgt tgcaggtaga tgtcgacacc gggatcggat gggaacgacg
     8221 atcccgtcca gtcggtttcg gtgagcggct tcccttcgac aagtttgtgg ccgatcgcgg
     8281 gatcgaaaca cagcggccgc gtcatgcctt ctgcgtggtg tgcggctagg gcggcgagga
     8341 tacggcgcgc cgcgtcagac tccaggggtg gataacgggt tacgatcgtt ttggttcctt
     8401 caaagacgcc ccaggtggtg acgcccggtt tgacggcgca ggcggcgagg tgggtgatcc
     8461 aggccgttac gatatccttt ggtttttctt tggcaggccg cgtaaaaagc tgggcgttgc
     8521 cggcccgcaa cgcgttcagg gtgccctgca gccggaaccc gttgggcaga tcgagatcga
     8581 acggctgggg ggcgagcggc tcgccgagat caagcgcgtt gactttttgg atcaccacgt
     8641 ctatccggtc acgggcctct gccatgtgtc ggtcggcgtc aaccggtagc ctgccgtcag
     8701 tctgccaacg gtcacgcaga cggtttatgg caagctcacc gtcgccgtgc aacatcgctg
     8761 cggtcaattc ctgccggatc tggtattgga cgagcgtgtc aggctcaagc gcttcgaggt
     8821 catccggcaa ttcgtcgtcg cgttccgtca gggcggcgtt gagacgccgc gtgtagaagg
     8881 ctttgcatgg cgagaggaaa aaagcgatca gttcttcaag ttggagcggc gcgtcggtcg
     8941 cggcggattc cgctgacgga aaaacaacct gtgcctcggg ctgtggtgat gcgtctccgg
     9001 tcgcgccggc caggcgacag gcgatgcgga aagcggtggc atcgaacgcg cggaggcgcg
     9061 ggtcgggctt gtcgcgtgtg aagtaggccg ggctgaacgg gtggagcggg tggcgcacga
     9121 tgagggctgt ggaggcggtt tgggggacac cgtcgcgaga cggaaaggtg aaggcggcgt
     9181 cgatggcatc gagcagttcg ctgaccagca ccgagggcgg caacggcttg ttgtctttgt
     9241 tgctctgtcc ggtataggtg atgatcagac gttcgcgcgc ggcgagcagg gtttccagaa
     9301 aggcgtaccg gtcctcgtcg cgtggccgac ggtcgccgat acaacggttt tcgcgtcggt
     9361 ccatcagatc gaaactcaac tgcggcgcgt tgcgggggaa ggccttgtca ctgagtccga
     9421 gcaggcagac cacgcgggca gggacgttgc gcatcggttg gaatcgcgag aaggtcacgc
     9481 ggccggtgac aaagcgttcg ctgcccggtg tgccgtcgag ctgagcggtg agccaggcgc
     9541 gtatcacaga gaaggggacc gcaagccgct cacagcccgc gtgttcgatg tagcgggcga
     9601 actgatcgag ggtttgccgc agcagcagca cgccgtaaga cgcgcgctcg tccgtgggga
     9661 agaaccgctc aagtgccgcg ttcaggtagg cgtgccacgc accgcaggga cgtgtcgggc
     9721 gggagaggca gaattcatgc agttccgcga gggcatcgag gaacgatgcg agacggccga
     9781 tcagcgaggc gtgaccttcg gcttcggtca atgggataca ctttccttcc gtcaggttca
     9841 gaggcggggc ttcatcagtc gggtcctgca tggcggcacc gaaaagaagg cggtcgaggg
     9901 caaaacgcca cgtgttggca taggccggac tggccccttg ccgtgcacga aaagccgcgt
     9961 ccaggcccca ggaaaggtgc gcggctctga gcaagctgcc gaggcacggc atgtcggtct
    10021 cgacgaagtt gaaccgttgg cggagtgcgg catgctggag cagtccgagc acctccgagg
    10081 ctttgaagcg tccggtaacg gcggtgagca gggcgagcca tgcccgacac tcgtccaccg
    10141 tgtgcagcag cgacttgtcc gcgatggtgt aggggatatg gccgggtgtg ccgggcggaa
    10201 cttttccgaa gacggcttcg atgtatgggg cgaaggtttc gatgtccggc gtgtagacac
    10261 tgatttcgcg cggcgcgagg ccgggttcat cgcggaacaa ggcgagcagg tggccgtgca
    10321 gggcctcgac ctcgcgcagc ggcgcgtgac aggcatggat caccagggag cggtcagagg
    10381 gggcaacagg ctgccgcggt tcgaggggcg tgttccggag gatcccgcgc tggagggttc
    10441 cgagcaccgt tccttcagcg gcatccggct ccacaaacag gcgttcgtcg tggatttcgt
    10501 cataggccaa gagcaacgag aagaattcgc gtccgctgcg tccgagcgag ccaagcagcg
    10561 ggttggcgcg gatctcatag gcgggggcga gttggggacc gattgtctga accagagtga
    10621 gccggtcgcg gatctgagtt ttcaaactgc gtgcgtcact ccactcctcg cagcaggggt
    10681 tgggggtgaa gaagtggatc tcgaagtcgt cgaaacgcgc caaacggatc aggatatcca
    10741 gatgagcggg cggcagggag gagacgccga aatacgcgat gcggcgccgg gccagcagcg
    10801 gggcgaggaa gtcgggctgc cggtcggggt gccgggtgag ctcgatgaag cggcggtaga
    10861 gtccggagaa gtgacgcacg cttccgcggt cgagcgcgat gagttcctgc cagaggcggg
    10921 cctgccatgc ggcgtcttca ccggtcagcg gttcgtcacc ttgttcccag gcggccaaca
    10981 aatcgggccg gtaggtcata tagcggtcgt agagcgatgc cagccgaccg gcgagttggt
    11041 aacggcgcag cgggtcgcag cgggtgaagg cgtggacgcg cgcgaacgcg gtttccggtt
    11101 cgagttgttc gagcagtgcg aacaggcgcc actccattgt ggcgggatcg aaaggcaggt
    11161 ccggttgaac agcgcgggag gttcgtccca tcattggatc aaacaggtcg cgatagagga
    11221 atgcaccggg ataggggagg tcgagcggcg gacagatttt ccgccgggcg gcgatttcga
    11281 gggcgagcca gcgggccagg ccctgattgg ggaccacgat tgtttcctgc cgcaacggat
    11341 cccgcaccgc accgcctgcg ggcggttgga ggatgaggtc cgcgagtgcc gccgcgagtt
    11401 gatccagccg gttgccgtga tacagcgtga aactcatagg gcggccctcc ccatcctccc
    11461 atccgtgaca tgcgtcgcct ccggcgtgcg gttctgcgcg cgcagccttg tcatgaggca
    11521 ttttttctga ggattccgtt tcatcgtctg ttacgagaac cttaatcaag aatatcgatt
    11581 atattgcggc atcatcacaa acgcaacgat gcggttccga aaaagatcgt tcgatttcgc
    11641 atgatcatcg ttgacggaac gtgtcaaaca caagtacaat catatgtaat tccatatcat
    11701 ttatcgacta tgggctgttt cgctgaggtg cgttgctctg aagtagggga gggatgacca
    11761 tgaagccagt tgttgtgatg acaggcgtgg ctgtcggttg gatcgttttt tctgcggcgg
    11821 cggagacgaa ttattggatc gcgacctcgg gcacgggcaa ctggaacgag ccagcgaatt
    11881 ggagcttgga ccgcgttccc ggagcggagg acacggtaat catcacaaat gccgccacaa
    11941 cgggaaacgg atataccgtt gaaatcgacc aggccccggt caacgtgttc cgggtgacat
    12001 gctccgcgcc cgagggccag atgctcacgc tgaatgtccg gaccaatctg acggtgaaca
    12061 acaacagttc ttctgatgcc gactggtcgt tcggtcccaa agggaatgtg gcgatcaaca
    12121 ttcattcaaa cgccgagttg gatgcgtact gccagattct gggcgcgggc gaaagcaaca
    12181 actataccgt tcttcccggc gggacattga gatacggcag gactcccagt aagcgcttcc
    12241 gggtgtatgg cagcctgacg gttcagggaa gcgtgatcgc cggtaaccgc gcaggcgaac
    12301 tgatttacgc gaatccgctc atattggatg gggggtatct gtttgcaaaa gccctcagtt
    12361 cacctccgga gctcgtgacg aacaatgcgt cgttgagtta ttcgttaatg aggattgcgg
    12421 gaatgacggt cacgatgcgg gacgggacgg tcacgaaccg cgcggacgac gcgctgcaca
    12481 tcggaaacgc cgcctccgga aatgcgtcgg cgtttctgaa tttgtcgggg gggctctggc
    12541 ggcaggaggg gcccgcctac atctgcgagc ggccgaacgg gtatctggat ctgtcaggta
    12601 cggcccaatt cgtctccacc aacaacgtgt gggtgggatc caacaacacg ggcacccaac
    12661 cgttcctgcc cggctacagc gggttcgtga cggttcattc aggcaacttc tgcgtgacca
    12721 attccgacgg aagtgcaagg ctgtatctcg gcaacatcac gcccggctat ttgacgtgcg
    12781 cgggcggcac ttcgtcggtg gatcggctcg tgttgtctaa tcggggaaac gttttagggg
    12841 ccgacgcggt cgggcggatc acgatggcgg gcggacggct ggacatcctg cagtcgctgt
    12901 ccgcgacaaa cggcagttac tcggtgctgg cctgttcgac gggcacgctc aatgtggcca
    12961 acgcggacgt cgccaacgac agcgcgctgg tcatcggtga cgggaaaggc gaggctacgc
    13021 tcggcttgct ctccggcacg caccgctttg ccgacggtct ggtgatcacg aacaacgcca
    13081 cgctcagtgc cggcggggtc gggaaccggg cttccgcaga ggtcacgggg aatctcgcgc
    13141 tggccgaagg cgcgacgctg cactgcgatt ttcaggacaa cgcgggtgac agcctttcct
    13201 tggccggcaa gctgaccttg ccgacgcaag ccgcagtcac gctgacctcg ttggacggca
    13261 ctctgccgca acacgttgtt ttggtggccg ccgaggcgct tgacggagcg gtggaccttt
    13321 cggggtggac ggtcgcgtca atcggcgaga tcgggtaccg cgtggcggtg gaaggggcct
    13381 cgctggtgct gctcaagaaa ccgaagggta tggtgatcag cgtgttgtaa c
//
LOCUS       DAOSLE010000074        13058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4834_length_13058_cov_7.554949, whole genome shotgun sequence.
ACCESSION   DAOSLE010000074 DAOSLE010000000
VERSION     DAOSLE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13058
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4834_length_13058_cov_7.554949"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1205)
                     /locus_tag="PK576_06425"
     CDS             complement(294..1205)
                     /locus_tag="PK576_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61266.1"
                     /translation="MRTSRRLSGGRRAASLCLAFALAGLFCIVAGEAVHHDRTTSGAV
                     GLNIAAGFAIALSLALGLIAVAVQQRQWSRRARNRLVLGFPIALLSLLMLVVDAASRG
                     SARGGLGKAGPSPSALSSPEPVRLAPLFQSGWYGEAHQDGLLLVVTSFHTNSVESRAF
                     NRALRKPVSYATLSVINLGSAAPVVLRRAEVFFLLDSGETQSSLEVKPLLAPHSEHAA
                     LRKRLAVPREVKGGGMVADLPICVERDFPWERVAAVLISLHDRTCTIPGRMMTEKEKR
                     ALLEQNTERASTANTTNRDAEAWFKHL"
     gene            complement(1376..2080)
                     /locus_tag="PK576_06430"
     CDS             complement(1376..2080)
                     /locus_tag="PK576_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOE61267.1"
                     /translation="MATVMLIFLGARDRLLADGVATLAAHAGVPPETVVQLTDLAEQA
                     LPRGREQIRARFRGEEPLVIGCSRPRAVRALLDFAGVDTTARPIEWVALPFDREALRR
                     ESGVPWYPVIDRTLCTGCGTCREYCLFSVYGRTPGEQGVGPVRVVEPLYCKTGCPACA
                     RLCPVGALIFPFCPEAELNGEIETPAPQSNADLKRQLGEDPLRLLTERRKRRGLLDLS
                     KFEQAEEDRIRHSGVL"
     gene            complement(2091..3212)
                     /locus_tag="PK576_06435"
     CDS             complement(2091..3212)
                     /locus_tag="PK576_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOE61268.1"
                     /translation="MLYWIAPYLTASWGPFRLFSSHLMLLAAGTLAGGIVVWLLLPRL
                     WSRLPTDRGKSLAADGGLKSAGKPTGAGLLLALLLLPVLAVFVPMGRWEAGVVACLYA
                     SMVFGFLDDRSMCPWGEWKKGILDLVVALAAALCLYQGQGSLLWMPFFKSTLTLPPAL
                     YVTGAAALLWFTMNATNCSDGVDGLAGSLTLLSLFALAGFLYGVIGYRPIAEYLLIPH
                     NPDGARWAILVATVAGSLAGYLWHNAEPSRVLMGDAGSRLLGMLVGVAVLVAGNPFLI
                     VVVSPVVLVNGGTGLFKLILLRTLRWAGFDVRPPQTLPQEAAARQLAPVKMLHRIRFP
                     LHDHCRRNLHWSNAQVLMRFVLIQAFLTPLLFVILIKIR"
     gene            complement(3242..4126)
                     /locus_tag="PK576_06440"
     CDS             complement(3242..4126)
                     /locus_tag="PK576_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOE61269.1"
                     /translation="MQRLEERVQEGVLVCDGAMGTELHRLGLAPGECPEQWCLDHPEK
                     VRAVHQAYREAGSAIVETNTFGGNRYKLAHYGLESAVEAINRAGAALAREVAGDTQWV
                     MASLGPTGVFLEPYGEESEQAFYDAFAAQARAFEAGGADAVVVETMMAVEECCVAVRA
                     VRENTRLTCIASFTFDPQPDGGYASMMGVTPEQFAAQAQAAGAQILGANCGTGTDHMI
                     AIIRRLRAVAGVPLIAMPNAGMPMLVRGETVFPETPDEMAAQVGCLVEAGASIVGGCC
                     GTTPAHIAAIAAALNARR"
     gene            complement(4379..4678)
                     /locus_tag="PK576_06445"
     CDS             complement(4379..4678)
                     /locus_tag="PK576_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE61270.1"
                     /translation="MAKKATPKTKSEIMAALAAAGEISKKQASTVYEALLKIAYTGAK
                     DPKGIMLPGLGKLIKVKRAARKGRNPATGAEIKIPAKTVVKFRLAKAAKDAVLGK"
     gene            complement(4817..5707)
                     /locus_tag="PK576_06450"
     CDS             complement(4817..5707)
                     /locus_tag="PK576_06450"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOE61271.1"
                     /translation="MRDTVALYLDHLACERGLAANTRAAYARDLAAFGAFLEDRGITA
                     AGAVTREQVLAFLDSQARLKLSPATRGRRLVALKGFFAFLRAEGIVSSEVTEGMSGPA
                     KGLRLPHTLSEEEIVRLVESVRGTSVYDLRDRAMLELFYACGLRVSEGISLRVEDLRF
                     DESVVWCIGKGDKQRAVPLGSSARQALERYLDGSRPWFARNAAAERHLFLTRLGAPFT
                     RQGVFDMLVKRARAAGVGEAFSPHVLRHCFASHLLAHGAPLRAIQEMLGHADIATTQI
                     YTHIDEGQVVRTHARFHPRH"
     gene            complement(5718..6029)
                     /locus_tag="PK576_06455"
     CDS             complement(5718..6029)
                     /locus_tag="PK576_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOE61272.1"
                     /translation="MNRWITLCMLCVMMVTGCRVSDVRTHVVRVPGMAKQEDAEQIRK
                     ALLPLNGLQQDKTVFDLSQRKVTVTYDSMMIAHKNIEIAIAEAGYTANTIPAIRPASS
                     N"
     gene            6117..6887
                     /locus_tag="PK576_06460"
     CDS             6117..6887
                     /locus_tag="PK576_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61273.1"
                     /translation="MKAFLFLPAVLLLGLLIGAWGPKSDLLDARRKIADLEKRLAAQE
                     KNTRVDTLTRWVQIPDRARAERPRTTDPNRSSLSTNKTANASPVTNAPDTTPPDATTP
                     PDATPQRTAPAPEDLRARIDEAKELWRTRVDIARSQWIARLNLTEAQTPLFDETIDLM
                     NENLYDALQRFADRLESGDPLTPEAGTRVMNEMTAVMVETYDTLASFVPQDRQDETSH
                     MELTDFIDPGVGEPLIAVQDKLRDMPRGTRRHPRGMPR"
     gene            6884..7789
                     /locus_tag="PK576_06465"
     CDS             6884..7789
                     /locus_tag="PK576_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE61274.1"
                     /translation="MKALRDIGLVAVHEGLDALRSRRALVVLLLYMAAALCNMHWSIN
                     LLHRLENELATALQLPASERAGVISTALWKSKPFQRMMRQMTRNDLVLQDITGKHPAE
                     LIYAFFAFFYTPLLVVLVAGNRIAEEIGSGSVRYTIVRISRAGWSVGKFLGQTMMIGL
                     ALLLSGLGAYAVARFRLAGSGAPGLLLNIVQWSARAWLYSFPFLGLAMGLSHLTRSAS
                     RATVLGILAITLCFVLSILIRHFSGESGWRAMLPAIGMLLPDGYKMCLWRRSVPPLVS
                     GGVTLVTLGFVYLLAGHACFRKRDV"
     gene            7792..8706
                     /locus_tag="PK576_06470"
     CDS             7792..8706
                     /locus_tag="PK576_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61275.1"
                     /translation="MDNAIETTGLVKRYGARRALDGFTLSVPRFAVFGLVGPNGAGKT
                     TWMMSVAGLTHLTGGSVNLLGSGAFDPQVHSGRLAILPQDSELPPESRPLELLTHFAR
                     IQGLKRQEAARSATHVLEAVHLGDRLHAPIRSLSHGMRKRVMIAQCFLGYPEVVLLDE
                     PLSGLDPREVENMRGFFTRQRGRQTIVISSHNLHDIERLCTHVAFIDKGRAVRVATLD
                     EVTGRAGKLIYRVTAMPTDRAALEAAVPDTTIAYDAERGEVVCHYNPTLLTAEAVNAR
                     LLPALLAQCGLLAVSRGDSLETEYLRSL"
     gene            8715..9182
                     /locus_tag="PK576_06475"
     CDS             8715..9182
                     /locus_tag="PK576_06475"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOE61276.1"
                     /translation="MNTPLQIVLLQPQIPHNTGAIGRVCVGLGCALHLIRPLGFHLSD
                     TYVHRAGLDYWNHVRLTVHDSWEAFLDTVQPPRLFFLSTRGARSLYACAFRPDDALIF
                     GNENRGLPKAFYARYGDALFQIPMPGDQSRSLNLANAVSIASYEAYRQLTLPR"
     gene            complement(9166..9597)
                     /gene="tsaE"
                     /locus_tag="PK576_06480"
     CDS             complement(9166..9597)
                     /gene="tsaE"
                     /locus_tag="PK576_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE61277.1"
                     /translation="MTPRTHTVRSVEETWRLAAEVAVGLEPGMVIALHGDLGAGKTTF
                     VQGLGQALGIRRPMTSPTFTVSTEYRTPAFTLVHMDLYRLNGPDDLLTIGYPEYLENG
                     AVVAIEWPERAGDLIPADALHVRFTLTGEAETRVIEISAAG"
     gene            complement(9594..10607)
                     /locus_tag="PK576_06485"
     CDS             complement(9594..10607)
                     /locus_tag="PK576_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE61278.1"
                     /translation="MRTRNVREDIGPIVVYQESAVPYFCLRPRQVARLRAAFPGTRVV
                     WCRTERAFVRNLPTVRVAVTWAFRQAWFEAAPQLRVVISSAAGRDLSPLTPPPRVTVW
                     HGTFHGALMAETVLGWMLAFNRGILSAYRYQIQGALWPREALYGQVRLLHGTHALIVG
                     FGHIGTAIGRLLKPFGVRVTGVRRTPQTRLPAGFGPEDAVVPIGQLDALLPEADHLIL
                     VLPSDTGTDRLINAARLARLPRHAVLYNVGRGNCLDEAALARALTTGRLRGACLDVFA
                     EEPLQPGSPLVADLPGLVRLPHASAFADEYMERFTDEVIARLAARSRRRTRSARAARE
                     GAE"
     gene            complement(10688..12553)
                     /locus_tag="PK576_06490"
     CDS             complement(10688..12553)
                     /locus_tag="PK576_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE61279.1"
                     /translation="MNGGRRIGILAMTIGCAGMLCGATLMIEAEAFAEKGGWVVDQQF
                     MDQMGSPFLMAHGLGKPVADAVTSCEIPEAGTYRVLVRTRDWAAPWERKDAPGKFRVR
                     INDTLLPQVLGTAGAEWGWHTAGKVTLKKGSARIALCDLTGFNGRCDALILTTEEDFV
                     PESEVARLEAFRRRIGTIRSAQTCETVDLIIAGGGIPGICMAISAARLGLAVALIQDR
                     PVLGGNNSSEVRVHLAGRMNLDPYPRLGDVVSEIGPRRGGNAQPGDYYEDERKIAAVR
                     AEKNIRLYLSTRALGVEKTGARITAVTARHIETGQEIRLAAPLVADCTGDGVIGVLAG
                     AEYRYGRESREETGEPTAVEKADTQTMGSSVQWYSREAEEPVAFPEIAWGLEFNDENC
                     ERVKMGEWTWETGMRLHQVHDFERIRDYGLLVVFSNWSFLKNRSEHKAEYAKRRLDWV
                     AYVAGKRESRRLIGDLVLTEQDIVNQVIYPDATACTTWTIDLHYPDPKNSRHFPGAEF
                     KSIAKHQAIYPYPLPYRCLYSRNVENLFMAGRNISVTHVALGTVRVMRTGGMMGEVLG
                     MAASLCKKHGCTPREVYTDHLGELQELMRRGVGSGREQPPQRYNLGGTLMKVKKE"
BASE COUNT         2544 a         4564 c         3706 g         2244 t
ORIGIN      
        1 aaacccggat catcccggtc ttcgcatccc aactcaggga tacgcccgga atcagaagcg
       61 ttaccaaccc tgctctcatg cgcacttcct ttccgtatta tccgttaccg acatcgttga
      121 aaccgcctaa cctctgtcgc ctacaacggc cggtagccat accaccctcg cccattatcg
      181 gctactcaac tctgtacggg gttagtatcg caaaacacgg taagtcttgg caagcctcgt
      241 aaacctaaaa ccgggcaccg gaaagggcaa ccgccggtta ttcaaatcac ggcttagagg
      301 tgtttgaacc aagcttcggc atcccggttg gtggtgtttg ccgtagacgc gcgttccgtg
      361 ttttgttcca acagcgcccg tttctctttt tccgtcatca tccggcccgg gattgtgcag
      421 gtccggtcat gcagcgaaat gagcaccgcc gccacccgtt cccaagggaa atcgcgttcc
      481 acgcagatcg gcaaatccgc caccatgccg ccgcctttca cttcgcgcgg tacggcaagc
      541 cgtttgcgca gggcagcatg ctccgaatga ggggcaagca gtggcttaac ctccagactc
      601 gactgggttt cgccggagtc gagcaggaaa aaaacctccg cacgtctcag caccaccggt
      661 gccgcgctcc ccagattgat caccgagagc gtggcgtagg agaccggctt gcgcaatgcg
      721 cggttgaacg cccgactctc cactgaattc gtgtgaaagg aggtgacgac caacagcaat
      781 ccgtcctgat gcgcctcccc gtaccaacca gactggaaga gcggcgcgag gcggaccggc
      841 tccggcgagg acaatgccga aggggacggc cccgctttcc ccagtccgcc gcgggccgag
      901 ccccggctcg cggcatccac caccagcatc agcagactca gcaacgcgat cgggaaaccg
      961 aggaccagcc ggttacgcgc acgacggctc cattgccgct gctgcaccgc caccgcgatc
     1021 agtcccagcg caagagataa ggcgatggcg aaccccgccg cgatgttcag acctaccgcc
     1081 ccggaggtcg tccgatcgtg atgcaccgct tccccggcca caatgcagaa gagtcccgcc
     1141 agcgcaaaag ccagacaaag cgaggccgcc cttctgccgc cggaaagacg acgtgacgtc
     1201 cgcacccctg cgaccgctcg tttagaacgc tccgcgcgca cagaattacg gtgatgatga
     1261 tggtgatgac cggatcgcga catgtcactc cgtctggttt tgtgataccg taccccacca
     1321 tgacagagag gtcaattccg tattcatccc cgaacgcgcg gaacggaacg accgctcaca
     1381 gcacaccgga atgacggatg cggtcttcct cagcctgttc gaacttggac aggtcaagca
     1441 accctctgcg tttacggcgc tcggtcaaaa gccgcagggg gtcttccccc agttgacgtt
     1501 tcaaatcagc gttggactgc ggcgcgggcg tctcgatttc gccattcagt tccgcttcag
     1561 gacagaacgg gaagatcagt gcgccgaccg gacagagccg cgcgcaggcg ggacatccgg
     1621 ttttgcagta caacggttcg accacacgga ccggacccac gccctgctcc ccgggcgtcc
     1681 ggccatacac ggaaaagagg caatactcgc ggcaggttcc gcaccccgta cagagcgtgc
     1741 ggtcgatcac cggataccac ggcacgccag attcccgccg gagcgcctcg cggtcaaacg
     1801 gcaacgcaac ccattcaatg ggtcgcgccg tggtgtcgac cccggcgaaa tcgagcagcg
     1861 cccgcacggc gcgcggccgc gaacatccga tcaccagcgg ctcctcgccg cggaaccgcg
     1921 cccggatttg ctcccgcccg cgcggcaacg cctgttccgc cagatccgta agctgcacaa
     1981 ccgtttccgg cggcacaccc gcatgggcgg caagcgttgc gaccccgtcc gccaacagcc
     2041 ggtcccgcgc acccagaaag atcagcatga cagtcgccac agagtcctcc ttaccggatc
     2101 ttgatcagaa tcacaaacag cagcggcgta aggaacgcct gaatcagaac gaaacgcatc
     2161 agcacctggg cattactcca gtgcagattc ctgcggcagt ggtcatgcaa cgggaagcgg
     2221 atccggtgca gcattttcac cggcgcgagc tgccgcgcag ccgcctcctg cggaagcgtc
     2281 tgcggcggcc gcacgtcaaa accagcccac cggagcgtgc gcagcagaat cagcttgaac
     2341 agacccgtgc cgccgttgac caacaccact ggcgagacca ccacgatcag aaagggattg
     2401 cccgccacca acacggccac ccctaccagc atacccagca ggcgcgatcc ggcgtcgccc
     2461 atcagcaccc ggctcggctc ggcattgtgc cacagatatc cggccagcga accggccacc
     2521 gtcgccacca aaatcgccca gcgcgcgcca tcaggattgt gcggaatcag cagatattcg
     2581 gcgatcggcc gatacccgat caccccgtag agaaacccgg cgagcgcgaa cagcgacagc
     2641 agggtcagcg aaccggcaag gccgtccacc ccatccgaac agttcgttgc gttcatcgtg
     2701 aaccacaaga gcgctgcggc ccccgtcaca taaagcgccg gaggcagggt cagcgtggat
     2761 ttgaagaacg gcatccacaa cagggaaccc tgcccctgat acagacagag cgccgcagcc
     2821 aacgccacca ccagatccag tatacccttt ttccactcgc cccacgggca catgctgcgg
     2881 tcatcgagga acccgaaaac catgcttgcg taaaggcacg ccaccacccc cgcctcccaa
     2941 cgccccatcg gcacgaaaac cgcaagcacc ggcaacagca acagggcgag caacagcccc
     3001 gccccggtcg gttttccagc cgatttcaac ccgccgtccg cagccaggct tttgccccgg
     3061 tcggtcggca ggcgcgacca caggcgcggc aacaggagcc acacgactat tccgcctgcc
     3121 aatgtgccag cggccaacaa catcagatgc gacgagaaca gccggaacgg cccccagctc
     3181 gccgtcagat acggtgcgat ccaatacaac atcggacttt tctccccctc ccgtttgcga
     3241 ttcagcgccg tgcgttcagc gcggccgcga tggccgcgat atgcgcgggc gtggtgccgc
     3301 aacagccacc gacaatcgag gctcccgcct ccaccaggca cccgacctga gccgccatct
     3361 catccggcgt ttcgggaaaa accgtttcgc ccctgaccag catcggcatc cccgcgttcg
     3421 gcatggcgat cagcggaacc ccggcgaccg cgcgcagacg ccggatgatc gcgatcatgt
     3481 gatccgtccc cgtcccgcag ttcgcgccaa ggatctgcgc gccagccgcc tgcgcctgcg
     3541 ccgcgaactg ctccggcgtg acccccatca tcgaggcata gccgccatcc ggctgcggat
     3601 cgaaagtgaa actcgcgatg caggtgagcc gcgtgttttc ccgcacggcg cgcacagcca
     3661 cgcaacactc ttcgacagcc atcatggttt ccacgaccac cgcatccgcg cctccggctt
     3721 cgaacgcgcg ggcctgcgcg gcaaaggcgt catagaaggc ctgttcgctc tcctcgccat
     3781 acggctccag aaaaacaccg gtgggaccca gactcgccat cacccattgc gtgtctcccg
     3841 ccacctcccg cgccagagcc gcgcccgcgc ggttgatcgc ctcaaccgcc gactccaaac
     3901 cgtaatgcgc gagtttatag cggttcccgc caaaggtatt ggtttccaca atcgcgctgc
     3961 ccgcctcgcg gtacgcctga tgcactgccc gcaccttttc gggatgatcg aggcaccact
     4021 gttcggggca ttcgcccggc gcaagtccca accggtggag ttcggtcccc atcgccccgt
     4081 cacataccag cacaccctct tgaacccgct cctccaaccg ttgcatccgc acgcctcccc
     4141 cttctgccgg acccgaatcc cggcggtcgc acaatacccc gtgttgcccg ctccgggaaa
     4201 accaccgcct tccgcccgca tcgccatcag cttagcaaaa acccgcacac aacataaagc
     4261 gtaatggcaa tgggttcgtg atgggagaga acgtgaagat catgcgtttc tgatgaaaaa
     4321 agaaagacac cgcatcgaac aatgcggtgt cacgtaaagc catcgcttgt gcgaggcgtt
     4381 atttgccaag cactgcgtcc ttggcggctt tcgccagacg gaatttcaca accgtcttcg
     4441 cggggatctt gatctccgcg ccggtggcgg gattgcggcc cttacgggcg gcgcgcttga
     4501 ccttgatcag tttgccgagc cccgggagca taatgccctt gggatcttta gcgccagtgt
     4561 aggcgatctt caacagagct tcatacaccg tgcttgcctg ctttttgctg atctcaccag
     4621 ccgcggccag agcggccatg atttcgcttt tcgtcttggg cgttgccttt ttagccatac
     4681 gttaactcct tttcgttaat gggaaaaacc ccacaacagt ccacaacata gccttttttg
     4741 ttaaagaagc aaccacaaaa acgaaaaacc cgcgaaaaac cgcaggcacc ccttgatcct
     4801 tatcgcgcgc aaccggtcag tgtcgcgggt gaaaccgggc atgggtgcgg accacttggc
     4861 cttcgtcgat gtgcgtgtaa atctgggtgg tcgcgatatc cgcgtgaccc aacatctcct
     4921 gaatcgcccg cagcggcgca ccgtgcgcca gcaggtgact cgcgaagcag tgtcgcagca
     4981 catgcggcga gaacgcctcc ccgacacccg cagcccgcgc gcgtttgacc agcatgtcaa
     5041 acacaccctg ccgcgtgaac ggcgcgccaa gccgggtgag gaacagatgc cgctccgccg
     5101 ccgcgtttcg cgcgaaccac gggcgcgaac cgtccagata gcgttccaac gcctgccgcg
     5161 ccgaactgcc caagggaacc gcgcgctgct tgtcgccttt accgatgcac cacaccaccg
     5221 actcgtcaaa ccggagatcc tcgacacgca gtgaaatccc ttccgacacg cgcagtccgc
     5281 aggcgtaaaa caactccagc atggcccggt cgcgcaaatc gtacaccgac gtcccccgaa
     5341 cggactcgac caaccggaca atctcctcct ccgaaagcgt gtgcggaaga cgcaatccct
     5401 tggccggacc ggacatgccc tccgtcacct ctgaagacac gatcccttcc gcgcggagaa
     5461 aggcgaagaa ccctttcaag gcgacgagcc gtcgtccccg cgtcgcgggg ctcagcttca
     5521 gacgggcctg cgagtcgaga aaagcgagca cctgctcgcg cgtgaccgcc ccggccgccg
     5581 tgatcccgcg gtcttccaga aacgcgccga acgcggcaag gtcgcgcgcg tacgcagcgc
     5641 gggtgttggc ggcaagcccg cgctcgcagg cgagatgatc gagatacagc gccacggtgt
     5701 cgcgcatagc cgcgctccta attggaggaa gcgggacgga ttgccggaat ggtgttcgcg
     5761 gtatatcccg cttccgcaat cgcgatctcg atgttcttgt gcgcgatcat catggaatcg
     5821 tacgtcacgg taaccttgcg ctggctcaga tcgaagaccg ttttgtcctg ctgcaaaccg
     5881 ttcagcggga gcagcgcttt ccggatttgc tccgcatctt cctgcttcgc catccccggc
     5941 acccggacca catgagtccg gacatccgac acgcggcatc ccgtgaccat catgacgcac
     6001 aacatacaga gggtgatcca tcggttcatg catgtatcat agccgaaccg gacgcgcccc
     6061 cgcaaacgca aacttttcgc ttatcgcccg gtatccgtct ggtgtatcat ccacgtatga
     6121 aggctttcct ctttttgccg gccgtgttgc tgctgggact gctcatcggg gcctggggac
     6181 cgaaatcgga tctgctcgac gcgcgccgta aaatcgcgga cttggagaaa cgcctcgccg
     6241 cacaggaaaa aaacacgcgc gtggacaccc tcacgcggtg ggtgcaaatc ccggaccgcg
     6301 cccgtgccga gcgcccccgg acaaccgacc ccaaccggtc ctccctttcc accaataaga
     6361 cggctaacgc gtctcccgtc accaatgcgc cggacacaac gccgccggac gcaacgacgc
     6421 cgccggacgc aacgccccaa cgcacggcac cggcgcccga ggacctgcgc gcccggatcg
     6481 acgaggccaa agagttgtgg cggacgcgcg tcgatatcgc gcgctcgcag tggatcgcgc
     6541 gccttaacct gacggaggcg caaaccccgc tgttcgacga gacgatcgat ctgatgaacg
     6601 agaacctcta tgacgcactg caacgctttg ccgaccggct ggagtccggc gatccgctca
     6661 cacccgaagc cggcacacgg gtcatgaacg agatgaccgc cgtcatggtc gagacctatg
     6721 acacgttggc ctccttcgtc ccgcaagacc ggcaggacga aacgagccac atggagttga
     6781 ccgatttcat cgatcccggc gtgggcgagc cgttgatcgc cgtgcaggac aaactccgcg
     6841 acatgccgcg cggcacgcgg cgccaccccc gggggatgcc gcggtgaaag cgttgcgcga
     6901 catcggactg gtggccgtcc acgaggggtt agacgccctg cgcagtcgcc gcgcgctggt
     6961 ggtgctgttg ctttacatgg ccgccgccct ctgcaacatg cactggtcga tcaacctgct
     7021 gcaccggctg gaaaacgaac tcgccacggc actgcagttg ccagcgtccg agcgtgcggg
     7081 cgtgatctcc accgccctct ggaaatccaa gccctttcag cgcatgatgc gtcagatgac
     7141 ccggaacgat ctggttctgc aagacatcac cggcaaacat cctgcagaac tgatctacgc
     7201 ctttttcgcc tttttttaca ccccgctgct ggtggtgctg gtggccggca accgcatcgc
     7261 cgaggaaatc ggctctggat cggttcgata caccatcgtc cggatctcgc gtgccggatg
     7321 gtcggtcggt aaatttctag ggcagacgat gatgatcgga ctcgcgctgc tgttgagcgg
     7381 gctcggcgcc tatgcggtag cccgcttccg gttagcggga tcgggcgcgc ccggcctgct
     7441 gctcaacatc gtgcagtggt ccgcgcgcgc ctggctctat tccttcccgt tcctcggctt
     7501 ggcgatgggc ctctcccatc tgacccgttc ggccagccgc gcaacggtgc tgggcatcct
     7561 cgcgatcacc ctctgcttcg tgctctcgat cctgatccgg catttcagcg gcgagagcgg
     7621 ctggcgcgcg atgctgcccg cgatcggcat gctgctgccc gacgggtata aaatgtgcct
     7681 ttggcgccgg tcggtgccgc cgctggtgtc cggcggcgtc acgctggtga ccctcggctt
     7741 cgtttatctg ctggcagggc atgcgtgctt ccgcaaacgc gatgtgtgat catggacaac
     7801 gcgattgaaa ccaccggact ggtcaaacgc tacggcgcgc ggcgcgcgct ggacgggttc
     7861 acgctttccg tgccccggtt cgccgtgttc ggcctggtcg gccccaacgg cgcagggaaa
     7921 accacctgga tgatgtccgt ggcggggctc acgcacctga ccggcggcag cgtgaacctg
     7981 ttgggcagcg gcgcgttcga cccgcaggtt cacagcggac gcctcgcgat cctgccgcag
     8041 gattcggagc tgccgccgga aagccggccg ctggaactcc tgacccactt cgcgcggatc
     8101 caaggactga agcggcagga ggcggcacga tccgcgacgc acgtcctcga ggcggtgcat
     8161 ctcggcgacc gcctgcacgc accaatccgt tcgctctcgc acgggatgcg taaacgcgtg
     8221 atgatcgccc agtgttttct cggttatccg gaagtggtgt tgctggatga gccgcttagc
     8281 ggcctcgatc cccgcgaggt ggaaaacatg cgcggctttt tcacgcggca gcgcggtcgc
     8341 caaacaatcg tgatcagttc gcacaatctc catgacatcg aacgcctctg cacgcatgtc
     8401 gcatttatcg acaagggccg tgcggtacgt gtcgccacgc tggacgaggt gaccggcagg
     8461 gcggggaagc tgatttaccg cgtcaccgcc atgccaaccg atcgcgccgc gctggaggcg
     8521 gcggtgccgg atacaacaat cgcgtacgat gcggagcgcg gtgaagtcgt ctgtcactac
     8581 aacccgacac tgcttaccgc cgaagccgtc aacgcgcgcc tgctgccagc gttgctggcg
     8641 cagtgcgggc tgctcgccgt atcccggggc gacagtctgg aaacggaata tctccggagc
     8701 ctgtgacgca aaccatgaac accccgctac agatcgtgtt gcttcagccg cagatcccgc
     8761 acaacacggg cgccatcggc cgtgtatgcg tgggattggg ttgcgccctc catctgatcc
     8821 ggccgctcgg gttccatctt tcggatacct acgtgcaccg ggcggggctg gattactgga
     8881 accacgtccg gctcacggtt cacgactctt gggaggcgtt tctcgatacc gtccaaccgc
     8941 cgcggctctt cttcctcagc acccgtggcg cacgcagcct gtacgcctgc gcattccgcc
     9001 cggacgacgc gctgatcttc ggcaatgaga accgcggcct gccaaaagcg ttctatgccc
     9061 gttacggcga cgcactgttc cagatcccca tgcccggcga tcagtcccga agtctcaacc
     9121 tcgcgaacgc ggtctccatc gcctcttacg aagcctaccg ccaactcacc ctgccgcgct
     9181 gatttcaatg acgcgcgttt ccgcctctcc ggtcagcgtg aaccgcacat gcagcgcgtc
     9241 tgccgggatc aggtcgccgg cccgttccgg ccactcgatt gccacaaccg cgccgttctc
     9301 gagatactcg ggatagccga tcgtcagcag atcatccggg ccattgagcc ggtagagatc
     9361 catatggacc aaggtgaacg ccggtgtccg gtattcagtg gaaaccgtga aggtcggact
     9421 cgtcatcggc cgccggatcc ccagcgcctg tcccagcccc tgcacaaagg tggttttccc
     9481 cgcgcccaaa tcgccgtgca gcgcgatcac cataccgggc tccaacccaa ccgcgacctc
     9541 cgcagccagt cgccaggttt cctcaaccga tcggaccgta tgcgtgcgcg gcgtcattcc
     9601 gccccctccc tcgcggcgcg ggccgatcgc gtgcgcctgc ggctccgcgc ggccagccgg
     9661 gcgatcacct catccgtgaa acgctccata tactcgtcgg caaaagcgga agcgtgcggc
     9721 aggcgcacca gtccggggag atccgcaacc aacggcgacc ccggctgcag cggctcctca
     9781 gcgaagacat cgaggcacgc gccgcgcagt ctgcccgtgg tcagcgcgcg cgccaacgcg
     9841 gcctcgtcca gacagttgcc gcggcccacg ttgtagagca ccgcgtgacg cggcaaacgc
     9901 gccaaccgcg cagcattgat caaccggtcc gtgccggtgt ccgagggcag caccagaatc
     9961 agatggtccg cctcaggcag gagcgcatca agctgtccga tcgggaccac ggcatcctcg
    10021 gggccgaacc ccgcaggcag gcgggtttgg ggcgtccggc gcacacccgt cacccgcaca
    10081 ccgaacggtt tgagcaatct gccgatcgcg gtgccgatgt gcccgaaccc cacgatcagc
    10141 gcatgtgtgc cgtgcaacag gcgcacctgc ccgtagagcg cctcacgcgg ccagagtgcg
    10201 ccttgaatct gataacgata ggcagacaga atcccgcggt tgaaggccag catccacccc
    10261 agaaccgttt ccgccatgag cgccccgtga aaggtgccgt gccataccgt gacgcgcggc
    10321 ggcggcgtga gcggggaaag atcgcgcccc gcagccgacg agattaccac gcgcagttgc
    10381 ggcgcggcct caaaccacgc ttggcggaac gcccacgtca ccgccacccg gacagtcggc
    10441 aggttccgca cgaacgcgcg ttcggtccgg caccaaacca cccgcgttcc cggaaacgcc
    10501 gcgcgcaacc gcgcgacctg ccgcggccgc agacagaagt acgggacagc actctcctga
    10561 tagaccacga tcggcccgat gtcttctcga acgttgcgcg ttcgcatttt ctctcccgct
    10621 ccacaggcaa tgcctatggg tctccgcgtg ccgcaccgca cgctgttgcg ggcggcctga
    10681 tgcgtaacta ttccttcttc accttcatca gcgtaccgcc cagattgtac cgttgcggcg
    10741 gctgctcccg gcccgacccg acaccccggc gcatcaactc ttggagttcg cccagatgat
    10801 cggtatagac ctcgcgcggc gtgcatccgt gctttttgca gagtgaggcg gccatgccga
    10861 gcacttcgcc catcatgccg ccagtacgca tcacccgcac ggttccgagt gccacatgcg
    10921 tcacgctgat gttccggccg gccatgaaga gattctccac attgcgggaa tagaggcagc
    10981 ggtacggaag cggatacgga tagatcgcct gatgtttggc gatcgactta aactccgccc
    11041 ccgggaagtg ccgactgttt ttcggatcgg gatagtgcag atcgatggtc caggtcgtgc
    11101 aggcggtggc atcggggtag atcacctgat tgacgatatc ctgctcggtc agcacgagat
    11161 caccgatcaa ccggcgcgac tcccgttttc cggccacata cgccacccag tcgagacgcc
    11221 gtttcgcgta ctccgctttg tgttccgaac gatttttaag gaaagaccag tttgagaaca
    11281 ccaccaacaa accgtagtcc cgaatgcgct cgaagtcgtg gacctgatgc aggcgcatgc
    11341 cggtctccca agtccactcc cccatcttga cccgctcaca gttctcgtca ttgaactcca
    11401 agccccatgc gatctccgga aaggcgaccg gctcctcggc ctcgcgcgaa taccactgga
    11461 ccgaggagcc catggtctgc gtgtccgcct tctccaccgc cgtgggttcc cccgtctcct
    11521 cgcggctttc gcgaccgtaa cggtactcgg cgcccgccag cacgccgatc acgccgtcac
    11581 ccgtgcaatc cgccaccagc ggcgcggcca accggatctc ctgaccggtt tcgatatgac
    11641 gggcggtcac ggcggtgatc cgcgctccgg ttttctcaac ccccaacgca cgcgtgctga
    11701 gatagagccg gatgttcttc tctgcccgga ccgccgcgat tttgcgttca tcctcgtaat
    11761 agtcgcccgg ctgggcattg ccgccgcggc gggggccgat ctcgctgacc acatcaccga
    11821 gccgcgggta agggtccaga ttcatacgcc ctgccaaatg gacgcggact tccgagctgt
    11881 tgttgccgcc caaaaccgga cggtcctgaa tcagcgccac agccaatccc aaacgcgccg
    11941 ccgagattgc catgcagatg ccgggaatac cgccgcccgc gataatcaga tcgaccgtct
    12001 cacaggtctg cgcactgcgg atcgtaccga tccgccgccg gaacgcctcc agtctcgcga
    12061 cctcgctctc cggcacgaaa tcttcctcgg tggtcaggat cagcgcgtca cagcggccgt
    12121 tgaaaccggt caggtcgcat agcgctatcc gcgcgctccc cttcttcagc gtcactttgc
    12181 ctgcggtgtg ccagccccac tccgcacccg cggtgccgag aacctgcggc aacagcgtgt
    12241 cgttgatccg cacccggaat ttaccagggg cgtccttgcg ttcccacggc gcggcccaat
    12301 cacgcgtccg caccagtacg cggtaagttc cggcctccgg aatctcacag gaggtgaccg
    12361 catccgcaac cggcttaccc agaccgtgcg ccatgagaaa aggcgacccc atctgatcca
    12421 taaactgctg gtccacgacc caaccgccct tttcagcgaa cgcttccgcc tcgatcatca
    12481 gcgtggcgcc acacagcatc cccgcacagc cgatggtcat ggccagtatc ccgattcgtc
    12541 tgcctccgtt catctgtcct cccctgcttt tttcgcgccg tacgccaaaa accggctcac
    12601 ggcctttcca tacccgcgca cattcaaatc cttgtccgcc gtgacacgcg ccacggcaaa
    12661 agaccccccg tctcgaacat cggaatccgt tccgcatcct gtatccccgc ataggggata
    12721 caacacaaac aaaaaacgcg gttagtttat accacctccg catgcccgtc cagacagcaa
    12781 tgaacgcacg cacaggagac ctcacttggc aaagcaaatg cgactggggt ggtctcatcc
    12841 actacttccg aacggggttg atgcggccag tggcatttag ttgctccaag gggggcgggg
    12901 tcggcggaga gggacgggcg ggacaggggg gtccgaaggt catgcgtttc agggtcccat
    12961 tgcggcgcta tcgccatcgg gatcgggatc ggaatcgccg ccgtcattac acttgtcgcc
    13021 gcgggccact gcgtgggcgc aacagtcgat ttcgaacc
//
LOCUS       DAOSLE010000075        12846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4933_length_12846_cov_7.605035, whole genome shotgun sequence.
ACCESSION   DAOSLE010000075 DAOSLE010000000
VERSION     DAOSLE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4933_length_12846_cov_7.605035"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1536
                     /locus_tag="PK576_06495"
     CDS             184..1536
                     /locus_tag="PK576_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOE61280.1"
                     /translation="MNRRLFLKRAFAAGVSLTSRADAKPRAPRILLRSSWQVVNIGDI
                     AHTPGVLALIEKHLPGVEVILWASGDLTPEVAAMEHRRFPKLKIVKGRIGANGDASTP
                     ELAEALTWCDFLLHGSGPSLVAHKDVAAFVRHVRKPFGVYGITYAGGADPAQRDLMSQ
                     ARFLYFRDSVSLGRAREERIASPEMGFAPDGAFGCDLRNDAAAEAFLRANRLEPGRFL
                     CCIPRLRNTPYWKIRNRPMNAAAHAKHARNEALKEQDHAPLREAICAVTRTTGLRVLV
                     CPEDMSQMAVGREMLVNRLPEDVRRQVVWRESFWLTDEALSTYVRSAGLFGLEMHSPI
                     LCIGNGIPAIVCRFAEQTSKGFMWRDIGLDAWLFDFDQAADRARLPAAVLDMVQNPAD
                     TRARVAEARKRVEARQRETMAALADTLGYRGASGRTGVMIPNRMGSEERNVPGLAQVR
                     "
     gene            complement(1594..2382)
                     /gene="ccsA"
                     /locus_tag="PK576_06500"
     CDS             complement(1594..2382)
                     /gene="ccsA"
                     /locus_tag="PK576_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOE61281.1"
                     /translation="MMVKLTFQGGLIYLTLTLFVATALALRGGWRAIGRGFWFAGFIA
                     ALASVIWRGVHTGHPPMQNLFEFFLCMAAALWPLSQWSRWHNQLDTDLRDALLGVVIL
                     IPAGFVFSEEVRRLPPALQSPLFIPHVASYVAGYVMLARAALMALPLFRAATLDRMTL
                     ERTETAARQTAAVGFVLLTLGLLLGSLWGKICWGHYWQWDPKEMWSLATWLVYAGLFH
                     YRLRHGLRAPGTLAALLWLGLLFIVLTLTWINVSRLFVGMHNYA"
     gene            complement(2379..3146)
                     /locus_tag="PK576_06505"
     CDS             complement(2379..3146)
                     /locus_tag="PK576_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOE61282.1"
                     /translation="MRAVLKKILSPGVAAWACWVAALGAVALAFPGATRAGALMARRP
                     MLMGTAVLAVWIGLAGIRALRGRRAASALLHLGCACVMAGWLVGRVAERSATPERPVT
                     GAMAMIAGDVSDKLWEGAYLTNFVGRLPFTVKLEQFFVERYTRNAVDIDAGREAPVRE
                     YRSRITVAEPGREPYVANVRVNQPLRVQGYRIYQMSWGQSQDLYGRPVTYTVLQFIRD
                     PGLPLVYAGFVLLFAGVLLFALRVGCARGTAAREVPS"
     gene            complement(3143..4177)
                     /locus_tag="PK576_06510"
     CDS             complement(3143..4177)
                     /locus_tag="PK576_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HOE61283.1"
                     /translation="MRKRHYGQGGVLRGQELGVFAPESGLPPAAKGVVLSQTGWGSRE
                     QILRAGVGVQGTEVPQLWLCGGDGQLPPVYQRLVVEGVSPRLIRRGACTVGVGYEDEH
                     ADYCLLTGVLPPDLNASREAQTTAVFEMIEDVLRDEGMTFAQVARTWLYMDRILDWYD
                     PFNRARDAFFHSRKVYDGLVPASTGIGSANLYGSAITACAIAVRPKRSGSLTLTALPS
                     PLQCAALEYGSSFSRAVEWLTPEARTVMVSGTASIEPGGATAFVGDVRRQIELTMDVV
                     RAILTSRGMDWADVTRAVMYLKRAEYQEEWEQYLREHALEGMPLITIEADVCRDDLLV
                     ELEADAVKRS"
     gene            complement(4219..6135)
                     /locus_tag="PK576_06515"
     CDS             complement(4219..6135)
                     /locus_tag="PK576_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE61284.1"
                     /translation="MRAIKRIGLFVMLGGWVGAACAAECLSPEYLAVSPDGLTLYVTA
                     ATADKLLLVDPAAGRVKAEWTVRHETRRGFWGRLGFSTSSGAKRVAGLSGVAVAPDGT
                     VYVTGGGVNGFLIKLNAEGSVMGQVCLGHTPLAPVVAPDGATVYVPNRFSSNVAVVDV
                     ASMTVSATVPMLREPFAAALGKDGALLFVANHLPAVRATNDVVAASVSVIDTAQKTVV
                     RHLMLPNGSTGVRGICASPDGRFVYVTHTFGRYQLPTTQLERGWMNTAGLSVFDGVTG
                     DYVNTALLDDVDLGAANPWGVTVTSDGKTLVVAHAGTHELSLIDRPAFHARLEKAARN
                     EKVTEVTKSASDVPNDLSFLVTLRRRIRLPGNGPRGVVAVGDSVYAALYFTEELVAVK
                     LGGPVVKPVRVMLGASNAPPTDPVRRGEMFWNDATLCFQQWQSCTSCHPEGRMDGLNW
                     DLVNDGIGNPKQTKALVYCHLTPPTMVTGIRPDMESCNRKGLTHIQFVVRPEEDALCL
                     DAYVMSLKPEPSPYLLNGRLSPLAKKGEAIFKKAGCAECHPSAESGPQGEPLFTNLKK
                     YDLGLGVGNEEGREFDTPTLVETWRTAPYLYDGRALTMEEMLTVCNPKDTHGVTRALS
                     PEEIRALAEYVLSF"
     gene            complement(6226..7053)
                     /gene="aroF"
                     /locus_tag="PK576_06520"
     CDS             complement(6226..7053)
                     /gene="aroF"
                     /locus_tag="PK576_06520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE61285.1"
                     /translation="MLFERDQNGVEASGGEGKGVVRVDKVVFGGPQVVLIAGPCTVES
                     RNQLLDIAGAVKEAGAVMLRGGAFKPRTSPYTFQGLCAKGLEYLAEARAMTGLPFVTE
                     VLETAHLHDVAQVADMVQLGSRNMHNSVLLRAAGSCGKPVLLKRGMSARIEEYLLAAE
                     YVLQGGARHVVLCERGIRTFETMTRFSLDLNAVPLLKERTWLPVIVDPSHGTSVSRLV
                     TPMARAAVACGADGLIVEVHNRPEQALCDGKQSITLEAFAAMVKGVRDVAEAVGRTL"
     gene            complement(7183..8652)
                     /locus_tag="PK576_06525"
     CDS             complement(7183..8652)
                     /locus_tag="PK576_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOE61286.1"
                     /translation="MGGFFGVVAKEDCVADLFFGTDYHSHLGTIRGGMAVWDTKGFQR
                     AIHDISNSQFRSKFESDCRRFSGTCGVGVISDNEDQPLVIASHLGVYAIVTVGMISNI
                     GELVRELFASQPVQFSEMSRGGINPTELVSMLINSQATFSEGVRYAQQRIEGSCSMLL
                     LTDEGVLFAVRDRYGRTPVIVGHKAGARAVAMESCCFPNLGYEVLRELGPGEMVCCTP
                     DGVETVLPPGDEMAICSFLWVYFGYPPSSYEGRNVEMARYRCGGALARRNPVKADSVG
                     GVPDSGVAHALGYAVEAGIRYARPFVKYTPTWPRSFTPSDPRDRELIAKMKLIPVPGL
                     IQGKRLVFCDDSIVRGTQLCDQVKRLFDDGAKEIHMRIACPPLLYQCRFLNFSRTKSE
                     YDLVTRRTIREIEGDGADIASYRDPDGAPYQKMVGRIRKRLGLGSLAYQRLDDLVAAI
                     GLPKERLCTYCWTGEDITKPAGCSHGCARCAVPCAAGSA"
     gene            complement(8758..9768)
                     /gene="pta"
                     /locus_tag="PK576_06530"
     CDS             complement(8758..9768)
                     /gene="pta"
                     /locus_tag="PK576_06530"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOE61287.1"
                     /translation="MSILDSMIPRAQAAGRTLVLPEGLDPRVITAAVRAAQAGICKPV
                     VLGTPEEIAAAEVAAGTTLSTAGVSVIDYTRSELLPVLAEALYEKRKAKGMTQEEALE
                     TVRSRRLYFGNMLVAIDKAQGLVAGSIASTGDMLRSAFHCIGTAKGIRLASSTFILDL
                     PRPTATGESTLLFADCAVNPCPTAEELVDIAHATALTYRKLIGKQPRLAFLSFSTRGS
                     AKHELVDKVRRAAEQTRARFEEQRVDAVVDGEVQADAALVPAVGAAKNKGGAIQGDAN
                     VLIFPDLQAGNISYKLVERLAGATALGPVIQGLAKPVNDLSRGCSADDIVGTMCITAL
                     QG"
     gene            complement(9781..11010)
                     /locus_tag="PK576_06535"
     CDS             complement(9781..11010)
                     /locus_tag="PK576_06535"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE61288.1"
                     /translation="MALKKFDKVLVINSGSSSLKFTLFNIPTKAMLAKGMVERIGTPN
                     ANLIYQRDGEPKMTQGIAAENHGKALAMACKMLTDPEWGVIKSLKEVDAIGHRVVHGA
                     EEFSDSVIITEDVKASIKHCADLAPLHNPANLDGVVACETVFPNVPNVGVFDTAFHQS
                     MPKHAYLYAIPQRYYDEYRIRKYGFHGTSHKFVMLAAAETLKKPVEELNLITCHLGNG
                     SSIAAIKGGKVLDTSMGMTPLAGLIMGTRCGDIDPAIIFFLARKGLSVDEIDDALNKQ
                     SGLLGINGIGSGDMRDTVNAATQGKAAAENALNMFGHRAAFYIGAYYTLLGGADAIIF
                     TGGIGENSAPARKAILSRLEALGCKMDEARNKAVAGNAGVISTENSKLTALVIPTNEE
                     LMIARETFRVLSENLKK"
     gene            11194..12552
                     /gene="glmM"
                     /locus_tag="PK576_06540"
     CDS             11194..12552
                     /gene="glmM"
                     /locus_tag="PK576_06540"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOE61289.1"
                     /translation="MGKLFGTDGIRGQANTFPVNAELALKLGKALGTRLHASGYGSAR
                     AVIGKDTRLSGYMLETAITSGLVSAGCDVFLVGPVPTPAVAHLTRSLAADVGIMLTAS
                     HNPYDDNGIKIFNPFGYKLEDEEEESLTRLILDGDLSTDNIRSDQLGKAHRLDDARGR
                     YIEFAKSTIDNAKLSEFKVVMDCANGSAYNIGPWIFRELGATVIKTGVTPDGLNINAG
                     CGALYPECIAKLVQEHKADIGIALDGDADRVIFADANGAIVDGDRILAACAIAFKKQG
                     LLANDTLVVTGMSNLGLHEAMRKHGIRVEVTDVGDRYVIDAMRKGGHSLGGEKSGHLI
                     FHNHATTGDGIIGALQVMRMMKQEGKSLAELTDCMTEYPQRLVSLPIKEKRPIHEVPA
                     LTAVIQACERELQQAGRVIVRYSGTESKIRLLVEASESALVERWVERLTQAVEESMGD
                     AI"
     gene            12591..>12846
                     /locus_tag="PK576_06545"
     CDS             12591..>12846
                     /locus_tag="PK576_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61290.1"
                     /translation="MSRVGSRLWCVLGCTVLCGAAIADEAAQQRVETETLIISGTGWT
                     LQPLANGAKAFSNRAYVWADLPRDTATWRYTQIAGGEPAVV"
BASE COUNT         2545 a         4329 c         3749 g         2223 t
ORIGIN      
        1 tgaaaccttg aaaccaaaga gggttgaagg ttcaactgct ctttttaggt ttagcgtgcg
       61 tactctgaaa cacagacgtg atcgtttccc cgtccccgtc tcacgccttg ccgcggaggc
      121 gctgttttag taacgtgtcg gcacggctcg gacccgcact gcccgccatt caggaggaac
      181 gtcatgaacc gccgcctttt tctgaaacgc gcctttgccg ccggggtttc gttgacttcg
      241 cgcgccgacg cgaaaccgcg cgcgccacgt atcctgctgc gctcctcgtg gcaggtcgtc
      301 aatatcggcg acatcgccca cacacccggc gtgctagctt tgatcgagaa acatcttccc
      361 ggtgtcgaag tgatcctctg ggcttcggga gacctcacgc cggaagtggc ggccatggag
      421 caccggcgtt tcccgaagct caagatcgtc aaaggccgca tcggcgcgaa cggcgacgcc
      481 tcgacgcctg aactggcgga agccctgacg tggtgcgatt tcctgttgca cggttccggc
      541 ccgtcgctgg tcgcccacaa ggatgtggcc gcgttcgtcc ggcatgtacg taaacccttc
      601 ggcgtgtacg gcatcaccta cgccggaggc gccgatccgg cgcaacgcga cctgatgagt
      661 caggcccgct tcctatactt ccgtgactcc gtctcgctgg gacgcgcacg ggaagagcgc
      721 atcgccagcc cggaaatggg gttcgccccg gacggcgcgt tcgggtgtga cctgcgcaac
      781 gatgccgcgg ccgaggcgtt cctgcgcgcg aaccgcctcg aacccggccg gttcctctgc
      841 tgcattccgc gcctgcgcaa cacgccgtac tggaaaatac gcaaccggcc gatgaatgcg
      901 gccgcgcacg ccaaacacgc gcggaacgag gcgctgaagg agcaggacca cgcgccgctg
      961 cgcgaggcga tctgcgccgt cacgcgcaca accggcctgc gtgtgctggt ttgccccgag
     1021 gacatgagcc aaatggcggt gggccgggag atgctggtga accgtcttcc cgaggatgtg
     1081 cggcggcagg tggtttggcg tgaatcgttc tggctgacgg atgaggctct gagcacctat
     1141 gtgcgttccg ccggactgtt cggtttggag atgcattcgc cgatcctgtg catcggcaac
     1201 ggcatccctg cgatcgtctg ccgcttcgcc gaacagacga gcaagggttt catgtggcgc
     1261 gacatcggtc tggacgcatg gctcttcgat ttcgatcagg cggcggaccg cgcgcgcctg
     1321 cccgccgctg tgctcgacat ggttcagaat ccggccgaca cgcgcgcacg cgtggccgag
     1381 gcccgcaaac gggtggaagc gcgccagcgc gaaacaatgg cggcacttgc cgataccctc
     1441 ggataccgcg gcgcatcggg acgaacggga gtcatgatac cgaaccgcat ggggagcgaa
     1501 gaacggaacg tccccggcct tgcgcaggtc agatgaggcg ctttgaactt gaaacgcgaa
     1561 gtccccgcac ccgccggaac tcacgttacg caatcacgca tagttgtgca tcccgacaaa
     1621 aagccgcgac acgttgatcc aggtcagggt cagcacaatg aacagcaggc cgagccacaa
     1681 cagcgccgcc agtgtaccgg gcgcgcgcag accgtgccga aggcggtaat ggaagaggcc
     1741 ggcgtatacc agccacgtgg ccagcgacca catctccttg ggatcccact gccagtaatg
     1801 cccccagcag atcttgcccc acaacgaacc cagcaacaac cccagcgtca ggagcacgaa
     1861 cccgacggcc gcagtctggc gcgcggcggt ctcggtccgt tccagagtca tccggtccaa
     1921 cgtagccgcg cgaaagagcg gcaacgccat cagcgccgca cgggccagca tcacatagcc
     1981 cgcgacataa ctcgccacat gggggatgaa gagcggactc tgcagcgcgg gaggtagacg
     2041 gcgcacttcc tcgctgaaga cgaagccggc agggatcagg atcaccacgc ccaacagcgc
     2101 gtcccgcagg tcggtgtcca gttgattgtg ccaccggctc cactgcgaga gcggccagag
     2161 cgcggcggcc atgcagagga agaactcgaa cagattctgc atcggcggat ggcccgtatg
     2221 cacaccgcgc cagatgaccg aagccagcgc cgcaataaat cccgcgaacc agaacccccg
     2281 cccgattgcg cgccaccccc cgcgcaacgc cagcgcggtc gccacgaaca gcgtgagggt
     2341 caggtagatc agaccgccct gaaaggtcag cttcaccatc atgacggcac ctcccgcgcc
     2401 gccgtcccgc gcgcgcagcc gacgcgcaac gcaaagagca gcactcccgc gaaaagcaga
     2461 acaaacccgg catagacgag cggcaggccg ggatcgcgga tgaactgcaa gaccgtgtag
     2521 gtgacgggac gcccgtagag atcctgactc tgcccccagc tcatctggta gatgcggtat
     2581 ccctggacac gaagcggctg attcacacgc acgttggcca cgtacggctc ccggcccggc
     2641 tcggcgaccg tgatacggct gcggtactcg cgcaccggtg cctcacggcc cgcatcgata
     2701 tccaccgcat tgcgcgtata gcgctcgacg aaaaactgtt cgagcttcac ggtgaacggc
     2761 agacgcccca caaagttggt gaggtacgcc ccctcccaca gcttgtcgga gacatcgcca
     2821 gcgatcatcg ccatggcccc cgtgaccggc cgttccggtg ttgccgaacg ctccgccacc
     2881 cgtccgacca accaaccggc catcacgcac gcgcaaccga gatgcagcaa tgccgaggcc
     2941 gcgcggcgcc cccgcaacgc ccgtatcccc gcaaggccga tccagacggc cagcaccgcg
     3001 gttcccatca gcatggggcg ccgcgccatc aacgcgcccg cgcgcgttgc gccgggaaac
     3061 gccaaagcga ccgcccccag cgccgcaacc cagcaggccc acgccgccac gccgggagac
     3121 aggatttttt ttaagaccgc tctcacgaac gtttcacggc atccgcttcc aattccacca
     3181 gcagatcatc ccggcagaca tcggcctcaa tggtgatcag cggcatcccc tcgagcgcat
     3241 gttcccgaag gtactgctcc cactcttctt ggtattcggc gcgtttcagg tacatgaccg
     3301 cgcgcgtgac atccgcccaa tccatgccgc gggaggtcag gatcgcgcgc accacatcca
     3361 tcgtgagttc gatctgacgc cgcacgtcac cgacaaacgc cgtggcccct ccgggttcga
     3421 tgctggccgt gcccgagacc atcacggtcc gggcttcggg tgtgagccac tccacggcgc
     3481 ggctgaacga actgccgtat tcgagcgcgg cgcattgcag gggcgagggc agggccgtaa
     3541 gcgtgagcga accggagcgt ttgggccgga ccgcgatcgc gcaggcggtg atcgccgaac
     3601 cgtagaggtt cgcgctgccg attcccgtgc tggcggggac cagaccgtca tacaccttcc
     3661 gtgagtggaa aaacgcgtcg cgcgcgcggt tgaacggatc ataccagtca aggatacggt
     3721 ccatatagag ccaggttcgg gccacctgcg cgaaggtcat tccctcatcg cgcaacacat
     3781 cctcgatcat ttcgaacacg gcggtggtct gcgcctcgcg cgatgcgttc aaatccggcg
     3841 gcagcacccc ggtcagcaaa caatagtccg cgtgctcatc ctcatacccc acacccacgg
     3901 tgcaggcacc gcgccgaatc aaccgcggcg acaccccttc cacgaccagc cgctggtaga
     3961 ccggaggcaa ctggccatcc cccccgcaca accagagttg cggcacctcc gtgccttgga
     4021 cgccaacccc ggcacggagg atctgttcgc ggcttcccca tccggtttgc gacagaacaa
     4081 cacccttggc cgcaggcggc aagcccgact ccggcgcgaa cacgcccaac tcctgccccc
     4141 gcaacacccc accctgcccg taatgccgtt tccgcattct tcctccgcct ctggcgccgt
     4201 actccaacgc cctttcacct aaaacgataa gacatactcc gccagcgcgc ggatctcttc
     4261 aggcgacagc gcccgagtga ccccatgcgt atccttcgga ttgcacacgg tgagcatctc
     4321 ttccatcgtc aacgcgcgtc cgtcgtacag atacggcgcg gttcgccacg tttcaaccag
     4381 cgtgggcgtg tcaaactcgc gtccctcctc gttccccaca cccaatccga gatcatactt
     4441 cttcagattg gtgaacaacg gttcaccctg cggaccgctt tctgcggacg gatgacactc
     4501 cgcgcacccc gccttcttga agatcgcctc cccttttttc gccagcggtg acagccgccc
     4561 attgaggaga tagggactgg gctccggctt cagcgacatc acatacgcgt caaggcacaa
     4621 ggcgtcctct tcaggacgca ccacgaactg gatatgcgtc agccccttgc gattgcagga
     4681 ctccatgtcg ggacggatcc cggtaaccat ggtgggcggc gtgaggtggc agtacacgag
     4741 ggctttggtc tgtttcggat tgccgatccc gtcgttcacc agatcccagt tcagcccgtc
     4801 catacgccct tccggatgac agctcgtaca gctctgccac tgctggaaac agagtgtggc
     4861 atcgttccag aacatctcgc cgcggcgcac gggatccgtc ggcggcgcat tcgaggcacc
     4921 cagcatcacc cgcacgggct tcaccaccgg cccgcccagt ttcacggcga ccagttcttc
     4981 cgtgaagtac agggcggcat acacgctgtc ccccaccgcg accacgccgc ggggaccgtt
     5041 tcccggcagc cggatcctgc gccgcagcgt cacgagaaat gagagatcgt tcggcacgtc
     5101 cgacgcggat ttcgtcactt cggtgacctt ctcgttccgg gcggcctttt ccaaccgcgc
     5161 atgaaacgcg gggcggtcga tgaggctcag ctcgtgcgtt ccggcatggg ccacaaccag
     5221 cgtcttgccg tctgacgtaa ccgtgacgcc ccacggattg gccgcgccca gatccacatc
     5281 atccagcagt gcggtgttga catagtcgcc ggtcacaccg tcgaacacgc tgaggccagc
     5341 ggtattcatc cagccgcgtt caagctgggt ggtcggcaac tgatagcgtc cgaacgtatg
     5401 ggtgacgtag acgaaccggc catcgggcga ggcgcagatt ccgcgcacac cggtggagcc
     5461 gttgggcaac atgaggtgcc tcaccaccgt cttctgcgcg gtgtcgatca ccgaaaccga
     5521 agccgccacc acatcgttgg tcgcgcggac ggccggaagg tggttggcca caaacagcag
     5581 ggcgccgtcc ttgccgagcg cggccgcgaa cggttcgcgc aacattggca ccgtggctga
     5641 aacggtcatg gaagcgacat ccaccaccgc cacgttggaa gagaaacggt tgggcacata
     5701 caccgtcgcc ccatccggcg caaccaccgg cgcgagcggg gtgtgcccca gacagacctg
     5761 ccccatcaca ctgccttccg cattgagttt gatcagaaaa ccgttcacgc ctccgcccgt
     5821 gacgtacacg gtgccgtccg gtgccacggc cactccgctc agacccgcca cgcgcttggc
     5881 tccgctggac gtgctgaagc cgaggcggcc ccagaatccg cggcgcgtct catgccgcac
     5941 cgtccactct gccttgaccc gtccggccgc cggatcgacc aacagcaact tgtccgccgt
     6001 ggcggcggtc acatacagcg tgaggccatc cggagaaacc gccagatatt ccggcgaaag
     6061 gcactctgcc gcacacgccg caccgaccca tccccccaac atcacaaaca atccgattcg
     6121 ctttatggcc ctcatacccg ttctcctccg ttgtttgaca ccttcatcca cgaccttccg
     6181 gattttcttt tacgcgcgga caccgcaatg cccgcagggt caaagtcaaa gcgtgcggcc
     6241 cacggcctcc gccacatcgc gcaccccctt caccatcgca gcaaatgctt cgagcgttat
     6301 gctctgtttg ccgtcacaca gcgcctgttc cggacggttg tgcacctcca cgatcagccc
     6361 gtccgcaccg caggccactg cggcgcgcgc catcggcgtc accagacggc tcacactcgt
     6421 gccatggctg ggatccacga tcaccggcag ccaggtccgt tccttgagca ggggcacggc
     6481 gttcagatcc agcgagaagc gcgtcatggt ctcgaatgtc cggatcccgc gttcgcacag
     6541 caccacgtgg cgggcaccgc cctgcagcac gtattcagcc gccagcaggt actcctcaat
     6601 ccgggcgctc atcccgcgct tcagcagcac cggcttgccg cagcttccgg ccgcccgcag
     6661 caaaaccgag ttgtgcatat tgcgggatcc caactgcacc atatccgcaa cctgcgccac
     6721 atcatggagg tgggccgttt ccagcacctc ggttacgaac ggcaaacccg tcatggcgcg
     6781 cgcctcggcc agatactcca gacctttggc gcacagcccc tgaaacgtat agggtgaggt
     6841 gcgcggtttg aaagccccgc cgcgaagcat cacggccccg gcctctttaa ccgcgcccgc
     6901 gatgtccagc aactggttgc ggctctcgac tgtacacggg ccggcgatca gaaccacttg
     6961 cggtccaccg aagaccacct tgtcaacccg gacgacaccc tttccttccc cgccggatgc
     7021 ctcgaccccg ttttgatctc gctcaaaaag catgtttctc ccgccttcgt cccccgcgcg
     7081 gaccggcccc ccccgaacac gcgcaacacg caaatcccca aaacgatttg agtttatcac
     7141 acccgtctct tcacccgcaa gcatcgggat cgaacccgaa gcctacgcag aaccggcggc
     7201 gcacgggacg gcacagcgtg cgcaaccgtg ggagcatccc gccggtttcg tgatatcctc
     7261 tcccgtccag caataggtgc acaaacgctc tttcggcaat ccgatcgcgg ccaccagatc
     7321 gtccagacgc tgataggcca aggagccgag gcccaaccgt ttccggatac gccccaccat
     7381 cttctgatac ggcgcgccat ccggatcgcg gtacgaggcg atatccgcgc cgtcgccctc
     7441 gatctcgcgg atggtccggc gggtaacgag atcatactcg cttttggtgc gggagaagtt
     7501 gaggaagcgg cactggtaaa gcaacggcgg acaggcgatg cgcatgtgaa tctctttggc
     7561 cccatcatca aacaggcgct tcacctgatc gcacaactgc gtgccgcgca caatggagtc
     7621 gtcacagaaa accagccgct tcccttggat gagccccgga acgggaatca gcttcatttt
     7681 ggcgatcaac tcgcgatcac gcggatccga cggtgtaaaa ctgcgaggcc aggtcggggt
     7741 atatttcaca aacggacgcg cgtaccggat accggcttcg accgcataac ccaaggcatg
     7801 ggccactccg gaatccggca cccccccgac tgaatcggcc ttcaccggat tacgacgcgc
     7861 caaagcaccg ccgcaccggt aacgcgccat ctccacattg cgtccctcat aagacgaggg
     7921 gggatacccg aagtacaccc agagaaaaga gcagatcgcc atctcgtccc cggggggcag
     7981 gaccgtctcc accccgtccg gcgtgcagca caccatttcg cccggcccga gttcacgcaa
     8041 cacctcatat cccagattgg ggaaacagca gctctccatc gccaccgcgc gcgcgcccgc
     8101 cttgtgcccg acaatcaccg gcgtccgtcc gtagcggtca cgtaccgcaa acaggacacc
     8161 ctcgtccgtg agcagcaaca tggagcagga cccttcgatc cgctgctgcg cgtaccgcac
     8221 tccctcactg aatgtcgcct gcgagttgat gagcatcgaa accagttccg tggggttgat
     8281 gccgccccgg ctcatttccg agaactggac cggttgactc gcaaacaact cccggaccag
     8341 ttccccgatg ttggagatca tccccaccgt gacgatcgca tagacgccga gatgcgaggc
     8401 gatcaccagc ggctggtcct cgttgtcgct aatcaccccg acgccacacg tgccgctgaa
     8461 ccggcggcaa tccgattcga atttagagcg gaactgggaa ttggaaatat cgtggatcgc
     8521 ccgttgaaac cccttggtat cccacaccgc catcccgccc cgtatcgtgc cgaggtggga
     8581 atgatagtcg gttccgaaaa aaagatccgc gacacaatct tccttcgcga ccacaccgaa
     8641 aaaaccgccc atcgcaaccc tcccggaact gatatccctg tccgacaggc tacagggaaa
     8701 aagttagatt atcttaccgc ttttcagccc gtaccgccat tcaaaaagga cgtgcggtca
     8761 gccttgcagg gcggtgatgc acatcgtgcc gacgatatcg tcagcgctgc acccgcgcga
     8821 aagatcgttc accggcttgg ccagaccctg gatcaccgga cccaacgccg tggcgccagc
     8881 gagccgctcg accagcttat agctgatgtt gccggcctga agatccggga aaatcagcac
     8941 attggcgtca ccctgaatcg cgcccccttt gtttttggcc gcaccaaccg caggcacgag
     9001 tgcggcatcg gcttggacct cgccatccac gaccgcatcc acccgctgct cctcgaaccg
     9061 cgcgcgcgtc tgttccgcgg cgcggcgcac cttgtccacc aattcgtgtt tcgcgctgcc
     9121 ccgggtggag aaactgagaa acgccaaacg cggctgtttg ccgatcagct tacggtaggt
     9181 cagcgccgtc gcatgggcga tgtccaccag ttcctcggcg gtcgggcaag ggttcacggc
     9241 gcaatccgcg aaaagcagcg tgctctcgcc tgtggcggtg ggccgcggca gatccagaat
     9301 gaaggtcgac gaggcgagcc ggattccttt ggccgtaccg atacagtgga aagcgctgcg
     9361 cagcatgtcg ccggtcgagg cgatcgatcc cgcgaccagt ccctgcgcct tgtcgatcgc
     9421 gaccagcatg ttaccgaaat agaggcggcg gctccggacc gtctccagcg cctcttcctg
     9481 cgtcatgccc ttggccttgc gcttctcgta caaggcctcg gccagcacgg gcagcaattc
     9541 cgaacgggtg taatcgatga ccgacacccc ggctgtggac agcgtcgtcc cggcggccac
     9601 ctcggcagcc gcgatctcct ccggcgtgcc caacacgacc ggtttacaga taccggcttg
     9661 ggcagcgcga accgccgccg tgatgacacg cggatccaaa ccttcaggaa gcaccagcgt
     9721 cctgccggcc gcctgtgcgc ggggaatcat ggaatcgaga atgctcatag gggaaggtcc
     9781 ttatttcttg aggttttcgg aaagaacccg gaaggtctcc cgggcgatca tcaactcctc
     9841 gttcgtgggg atcacgagcg cggtcagctt cgaattctcc gtgctgatca ctcccgcgtt
     9901 cccggccacc gccttgtttc gggcctcgtc cattttgcac cctagcgcct ccagacgcga
     9961 gaggatcgcc ttacgggccg gcgcactgtt ctcgccgatc ccgccggtga aaatgatcgc
    10021 gtcggcgccg cccaacagcg tgtaataggc accgatgtag aaggcggccc ggtggccgaa
    10081 catgttcagc gcgttttcgg ccgcggcctt gccctgcgtg gcagcgttca cggtatcgcg
    10141 catgtcgcca ctgccgatgc cgttgatgcc gaggagcccg ctttgcttgt tgagtgcgtc
    10201 gtcgatctca tcaaccgaca gccccttgcg ggcgaggaag aaaatgatcg cgggatcaat
    10261 gtcaccgcaa cgcgtgccca tgatcagtcc ggcgagcggc gtcatcccca tcgacgtatc
    10321 gagcaccttg ccgcccttga tcgccgcgat ggaggagccg tttccgaggt ggcaggtaat
    10381 gaggttaagc tcctcgaccg gcttcttgag ggtttcggcc gcagccagca tcacgaactt
    10441 gtgcgaggtg ccatgaaagc cgtatttgcg gatccggtat tcatcgtaat accgctgcgg
    10501 aatcgcatag agataggcgt gcttgggcat actctgatgg aacgcggtgt cgaaaacccc
    10561 gacgttggga acattcggga agaccgtctc gcaggccacc acgccgtcca gattggcggg
    10621 gttgtgcaac ggcgcgaggt cggcgcaatg tttgatgctg gccttgacat cctcagtgat
    10681 gatgaccgaa tcggaaaact cctccgcgcc gtggaccacc cgatggccaa tcgcgtccac
    10741 ctctttcagc gatttgatca cgccccactc cggatcggtc agcattttgc aggccatggc
    10801 cagcgccttg ccgtggttct ccgccgcgat accctgcgtc attttgggtt cgccgtcgcg
    10861 ctggtagatg agattggcgt tcggtgtgcc gatccgctcc accatcccct tggcaagcat
    10921 ggctttcgtg ggaatgttga acaacgtgaa tttgagagag gaactgccgg agttgatgac
    10981 cagaaccttg tcgaatttct taagtgccat gatgaatcct tcgcgcctgc ggggtggtat
    11041 gtcatgatgc ggcacccgga ttcgtgtgcc gcgaaaaaca ggaactagtt tgcacttctt
    11101 tgccgtatgt ttcaagacca atccgcggaa aaggcttgcc gcgttcatgc cgttacggta
    11161 caaatcgtcc cagaacaacc gtagcaggtg aatatgggaa aactatttgg aaccgacggc
    11221 atccgggggc aggccaacac ctttccggtt aacgccgaac tggcgctgaa acttggcaag
    11281 gcgctgggta cgcgtctgca cgccagcggg tacggcagcg cgcgcgcggt gatcggcaaa
    11341 gacacgcgcc tctccggtta catgctggag accgccatta ccagcggcct ggtctcggcg
    11401 ggatgcgacg tgtttctggt cggaccggtg ccgacgcccg ccgtggcgca cttgacgcgt
    11461 tcgctggcgg cagatgtcgg catcatgctg accgcctcgc acaatcccta cgacgacaac
    11521 ggcatcaaga tcttcaatcc gttcgggtac aaactggaag acgaggagga ggagtccctc
    11581 acccgtctga ttctggacgg cgacctgagc acggacaaca tccgaagtga ccagctcggt
    11641 aaagcccaca ggctggatga cgcgcgcgga cggtacatcg agttcgccaa gagcacgatc
    11701 gacaacgcca agctgagcga attcaaggtg gtgatggatt gcgccaacgg ttcagcctac
    11761 aacatcggtc cctggatttt ccgcgaactg ggcgcgaccg tcatcaagac cggcgtcacc
    11821 ccggacgggc tcaacatcaa tgccgggtgc ggcgcactgt atcccgaatg catcgcaaag
    11881 ctggttcaag aacacaaggc cgatatcggc atcgcgctcg atggcgacgc ggaccgcgtc
    11941 atcttcgccg acgccaacgg cgccatcgtc gacggtgacc gcattctcgc cgcgtgcgcc
    12001 attgccttca agaaacaggg tctgctcgcc aatgacactc tggtggtgac cggcatgagt
    12061 aacctcggcc tccacgaggc gatgcgcaag cacggcatcc gggtcgaggt caccgacgtg
    12121 ggcgaccgct atgtgatcga cgccatgcgc aagggcggcc actcgctcgg cggcgaaaaa
    12181 tccggacacc tgatcttcca caatcacgcc acgaccggcg acggcattat cggtgccctg
    12241 caagtgatgc gcatgatgaa gcaggagggc aagagtctgg ccgaactgac cgactgcatg
    12301 accgaatatc cgcagcggct ggtcagcctc ccgatcaaag aaaaacgccc catccatgag
    12361 gtgccggccc tgactgcggt catccaagcc tgcgagcggg aattgcaaca ggcgggacgt
    12421 gtcattgtgc gctactcggg tacggagtcg aagatccgcc tgctggtcga ggccagcgaa
    12481 tcggcactgg tcgagcgttg ggtcgagcgc ctcacgcaag ccgttgagga atcgatggga
    12541 gacgcaatat gaagcaggcg ctacccgttc ttaatgacga agtgaaaagc atgtcaagag
    12601 ttggaagccg gttgtggtgc gtgttggggt gcacggttct ttgtggtgcg gcaatcgcgg
    12661 atgaggctgc gcagcaacgg gtggagacgg agacgctgat catcagcgga acggggtgga
    12721 cgctccagcc gctggcaaac ggcgcgaagg ccttttccaa tcgggcgtat gtgtgggccg
    12781 atctgccgcg tgacaccgca acgtggcgct acacgcaaat cgcgggcggc gagcctgcgg
    12841 tcgtgg
//
LOCUS       DAOSLE010000076        12830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4941_length_12830_cov_7.434442, whole genome shotgun sequence.
ACCESSION   DAOSLE010000076 DAOSLE010000000
VERSION     DAOSLE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4941_length_12830_cov_7.434442"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2532)
                     /locus_tag="PK576_06550"
     CDS             complement(112..2532)
                     /locus_tag="PK576_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61291.1"
                     /translation="MVGAFCAVLVCGWGSAQDTTVWTNPSGGVWNDAGNWNNGVPDGN
                     KASLTLNTASYIVDIESAPVPAIMNLTAQNTASQTTLLNVNTGTLAVTKGAILIETGA
                     EVCIKSNACLSFTGGSTTDFQGFFKTRSGGKLRVDGGSVCFTGVTFGSKGAIVGFCGA
                     GSAVVTSGVFQLSGSGNPPLHIARGENAKGYFEAAGSSRVDVAQLTIGMYGQPNSPAA
                     GRLEVKGDSVFTVGNSATVNVPLYNAPSGINNTGIVEVSENALLTCLGSYFDLGYATG
                     ATTNLGVLRVTGSGRFAAPNATLRCGNTDAGSAIFEVGDNGVCEIKNLYLNGNTTATL
                     NGNGRCFVTGSSAPRIGNGAACSNVVLQILDQALFAVTNATLYCGYDNSTAEIVVGSN
                     AVCMAQTSRFYGNSSLTVCGDGCYTGMVADTLNDSDYIGYNAGKVVLNVSGNGRFLTK
                     RNLNIGYFGAEGEVNVSGNGRLSTGCDFVIGYVNSSAASKAAVNLSGNGVIAPSVSGR
                     GLHMAVVGNSSTSVISNGMVQAWLNISDGLFDMTKDMWNADSFYEEGIIAGEIWKNNQ
                     GAAEAWINLSGGTVTNAGKMTAGMGLGATGVVTQTGGALNQGVTAAGGFNGRRTVLGW
                     GGGCGEYRLSGGTAVFGGSVYVGGITTNLLGYVLSDTKFTFEPDSAGLLRVEGGSLNI
                     TNNGTLWCGGYGRGAIEIGTGGVCVAKNIVLTNNTQSVVRFELGAEGCGVLRATDALR
                     VCEGARLEVDAQAYRSREVRLKLIDFGTRTGAFAPENVMVRGAGKVVQDEQGVWFYRP
                     RGMVVQIN"
     gene            2991..3488
                     /locus_tag="PK576_06555"
     CDS             2991..3488
                     /locus_tag="PK576_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOE61292.1"
                     /translation="MRAIQADITTLQVDAIVNAANTTLLGGGGVDGAIHRAAGPDLLD
                     ECRSLGGCAVGDAKITRGYKLPAKHVIHTVGPVWRGGNTGEEQHLANCYRRSLEIAQE
                     HGVKSIAFPAISTGVYGYPIEDATRVAVATVRAYLQESQSAMDVIFCCFSQRDLAVYD
                     KELAR"
     gene            3513..4367
                     /gene="zupT"
                     /locus_tag="PK576_06560"
     CDS             3513..4367
                     /gene="zupT"
                     /locus_tag="PK576_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HOE61293.1"
                     /translation="MRDVWIAFGLTLFAGLATGIGSMIAFLAKRTDYRFLSVATGFSA
                     GVMLYISFVEIFVKGNEALVARYGDPLGCWVNVASFFGGMLLIGLIDSVIPSAENPHE
                     IHDESETRVLREGGELEPHDDPAHGKKLMRVGVFTALAITIHNFPEGLATFLAALQDP
                     KLGAAIAIAVALHNIPEGISVSVPIYYATGNRRKAFCYSLLSGLAEPVGAGVAYFALR
                     LFAGGENGALPPEIMGILFGGVAGIMVYISLDELLPISRAYGKGHDSLFGVVGGMLVM
                     ALSLLLMR"
     gene            complement(4626..7553)
                     /locus_tag="PK576_06565"
     CDS             complement(4626..7553)
                     /locus_tag="PK576_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOE61294.1"
                     /translation="MKKVLISLFSVTALSFAACLDVRADEKDRRPVIFWASEPVRPGE
                     TAMVIGENLGDHPAIEIVRLPDGRAKEPLQENVVWPRGGTPVDVLQAAEQSAKFIVPA
                     DFKPGLLRLRLAAGGAAVEHIMNRPQIWWVQGECGPAVTPGGWIRFFGKNLGWGIKDG
                     IRKPVIRIEGPCVVSLAFETDGHAARAVLPKDMPSGSYRLFAHNGAGGRAGWSAPVEL
                     TVTNKEVWPQNVFNVTNFGALADGVKDDTVAIQNALSAAESQGGGIVYLPLGRYAVSD
                     TLNIPRRTVLRGERSDWVALAWSDMRNPPWALVQGTNSFGLEDLTIYAHHHHHVIAAD
                     LGDKPDAGNVFLRRVRVRASSYRGHLTPDEVDARFRETMKWSTGGGDTVRMGGSSIEI
                     TECDFYGSGRTLFLSRVRGGLVADNRFFNGRWGWYCISGSDGLIFENNEITGADLMST
                     GGGLNCLDGSRFSQNVYYADNRLQRMHGWDREAMTSDAGGEAYSGKAVTAHGTTLVLD
                     GKPNWRGQDWRGAGVFVLAGKGAGQYRRVAGQGEQSVIVDRPWDVVPGTNSDICVTMF
                     QGHYILSGNEFSDCGAVQFYGTAVENIVVRNTGTRMSGFRGIGLWYHGYQPNWFCEFR
                     DNAIKEGNYYHWNRASDALLEITGYRRAPYTGPLNVGSVVRHNRLDSQAHIRVGAATR
                     DAIVEGNHVANSAQGIFVSVHATNIWLHANTFEKVREEIMDDLKQRRIAQERLSRHPF
                     FNRPEPVAVWDFEKTERMNVADVSGNGFRARLNGGATTVSNGVGGRAGRFDGTGWLCV
                     NEVIVFNAPDMTVSLWIKPETLRGRRGLVGKRFSGCEAPFILSQNGARLSFEATDTEH
                     QWSFNFVSEPVLKEGQWTHVAAVVRRGKGVALFANGKKVAEKQNASDRIVNHEPLILG
                     REAWGGEPASTSTPGYFIGLMDEVRIWTRALSPDEIEKEASRRPAGNGFPRSSGSQPI
                     TKR"
     gene            complement(7611..8912)
                     /locus_tag="PK576_06570"
     CDS             complement(7611..8912)
                     /locus_tag="PK576_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOE61295.1"
                     /translation="MKKNIAIKLGLLSVLTVMAQEGGLPDVAPKAGWDTLAPVKKSEN
                     DWPWWRGPNFDNIAPPGQKPPTAWGAGSNILWQVKIPGVGHSSPCVVGNRIYITSGIK
                     TQGQTTLWLFCLDRNTGKTVWQAELYIGASAKVHADNSPASSTPACDGERVFVAYQTD
                     SSVVMVAVDVEGKIRWRQTVAPYNTIQGFSASPNIYKSAVIIPVEGPKGSYLTAFHRA
                     TGEVVWRTKLRAVNEGYAPAVVTVIAGREQLLQLGGTYTRGIDPENGTLLWECAGPAK
                     KVCVATAAFDRDTVYATGGYPNRILLAIRADGKGEVTKSHIRWSSDAKVGYVPSPILY
                     EGLLYAVADQGLLRCYDTVDGRVLWEQDFKVPFYSSPTLADGKLYLFDRKGKGYILPT
                     GRMAGAIVTNELPSGVFATPVFLENRIYLRTLGDFCCIGEK"
     gene            9212..11068
                     /locus_tag="PK576_06575"
     CDS             9212..11068
                     /locus_tag="PK576_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOE61296.1"
                     /translation="MKPLMSIQLFAFFAAAVAYGESVKPKIVDALQPITLKQSELSGE
                     IGRRINDLIYTNFMALDVDGFFVAPFRSRPFTNGYHYVGVGKVIEAGSLFAAYTGDPK
                     IADRTRHLIDGMIGTRDADGYIGVFKPEPDDRQNHWNWALHEQEYLLLGLTRNALLTH
                     DPKMLQDAQALADYMIRTFSKDPHPEQICTAGLPEAFLVLYKATGDTRYFDFAANIRH
                     GNGTAEIKCASLRDWRQTFETRPAHVYVMLARCYAQAELYRYDGVASLMDMSKYMRHE
                     LLRPGGGLNVVGSASDGEHFSYTQNGAGAIGESCVTAYLLRWLDSLMRLEGDLRYGDL
                     MERTVYNALFAAQDPAGRKLRYFTPFTGVRAYYGQDGFCCPGNYRRIVAELPQKVYYR
                     CSDGALSVNLFTASKVTVPVSPDATVTLAQKTDYPNTGHVVFRVTPSKETVFTLRLRI
                     PRWCRTATVTVNNESPLSVLPGAKAFELRRVWKTGDTVTLDMPMAWRFVKGHQLQEGK
                     AVLMRGPIVYCLGTAQNSEVLEKYPNFSGVVIDPTCIGEIENDTSIRPDGKKVKAQAR
                     VEAPGAGRKGSPHVTLTFTEFIDPTGIATYVHLLDLNKAEEDELVSDCFAKP"
     gene            11108..11692
                     /locus_tag="PK576_06580"
                     /pseudo
     CDS             11108..11692
                     /locus_tag="PK576_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
     gene            12359..12577
                     /locus_tag="PK576_06585"
                     /pseudo
     CDS             12359..12577
                     /locus_tag="PK576_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
BASE COUNT         2586 a         4026 c         3522 g         2696 t
ORIGIN      
        1 cccaagcaaa tccacatggg taagggtgaa ttatgccgcc tatgggatgc tagtgcctca
       61 attcattcct cccaacaacc ttcagcctat ttcttgcttt ctgttggttt gtcagttgat
      121 ctggaccacc ataccccgcg gccggtagaa ccagacaccc tgttcatcct gcaccacctt
      181 acccgcgccc ctcaccatga cgttctccgg cgcgaacgca cccgtccggg tcccgaagtc
      241 gatcagcttg agccgaacct cccgactgcg gtaggcctgc gcatcgactt ccagtctggc
      301 cccctcacaa accctcaagg catctgtggc ccgcaacaca ccgcacccct ccgcacccaa
      361 ctcgaaccgc acgaccgact gcgtgttgtt cgtgagcacg atgttcttcg ccacgcatac
      421 gccgcccgtg ccgatctcga tcgcgccgcg gccgtaaccg ccgcaccaga gcgtaccgtt
      481 gttggtgatg ttcagcgatc cgccctctac gcgcagcaac cccgcgctgt ccggctcgaa
      541 cgtgaacttc gtgtcggaca gaacataccc caacagattc gtggtgatgc cgccgacata
      601 cacgctcccg ccgaacactg ccgtgccgcc cgacaggcgg tactcgccgc acccgccgcc
      661 ccagcccagc accgtccgcc ttccgttgaa tcccccggcc gccgtcacgc cctgattcag
      721 cgcgccgccc gtctgggtca cgacacccgt ggcacccagc cccatgccgg ccgtcatctt
      781 gccggcgttc gtgaccgtgc cgccgctgag gttgatccac gcttccgccg ctccttggtt
      841 gttcttccag atttccccgg cgatgatccc ctcctcgtaa aaggaatccg cattccacat
      901 atctttcgtc atgtcgaaca gcccgtcgga aatgttcagc caggcttgca ccatcccgtt
      961 ggagatgacg cttgtgctgg agtttcccac caccgccatg tggagtccgc gccccgagac
     1021 tgagggcgcg atcacgccgt tgcccgagag gttgactgcg gcttttgacg cagcgctgct
     1081 gttcacgtaa ccgatcacga aatcgcaacc tgtagagaga cgcccgttac ctgaaacatt
     1141 cacctcgccc tccgcgccaa aataaccgat gttgaggttg cgtttcgtca gaaaccggcc
     1201 gttgcccgaa acgttgagca ccacctttcc ggcattgtac ccaatgtaat cactgtcgtt
     1261 aagcgtgtcc gcgaccatgc ccgtataaca accgtcaccg caaacggtca gcgaggagtt
     1321 cccgtaaaag cggctggttt gggccataca caccgcattt gaaccgacga cgatctccgc
     1381 tgtactgttg tcgtatccgc aatagagcgt ggcgttcgtc accgcaaaca gcgcctgatc
     1441 cagaatctgg agtaccacat tcgagcatgc cgcgccgttt ccaatccggg gcgcactgct
     1501 cccggtcaca aaacaccgcc cgttcccgtt cagggtcgcc gtcgtattgc cgttcagata
     1561 gaggtttttg atctcacaca cgccgttgtc gccaacctcg aaaattgccg accccgcatc
     1621 cgtgttgccg caacgcaggg tggcgttggg ggccgcgaat cgtccggagc ccgtaacacg
     1681 cagcacgccc aagttcgttg tcgcgcctgt ggcgtacccg agatcgaaat agctccccag
     1741 acaggtgagc agcgcgtttt cgctcacctc aacgatcccg gtgttattga tgccgctggg
     1801 ggcgttgtaa aggggaacgt taaccgttgc tgaattcccc accgtaaaga cggaatcgcc
     1861 cttcacctcc agtctgcccg cagcgggcga gttcggttgg ccgtacatgc cgatggttag
     1921 ttgtgccaca tcgacccggc tggaacccgc ggcctcgaaa tagcctttcg cgttttcgcc
     1981 gcgggcgata tgcaacggcg gattcccgct gccgctcaac tggaatacgc ccgaggtgac
     2041 gacagcggag cccgcgccac agaatccgac gatcgctcct ttcgaaccga acgtcacacc
     2101 cgtgaaacag accgagcccc cgtccacccg tagtttcccg ccactccggg ttttaaaaaa
     2161 gccttggaag tccgttgtcg aaccgcctgt gaagctcagg caggcattgc tcttaatgca
     2221 aacctcggct ccggtttcga tgagaatggc gcccttggtg acggccagcg ttccggtgtt
     2281 cacattcagg agcgtggtct gcgaagccgt attctgcgcc gtcagattca tgatcgcggg
     2341 caccggcgca ctctcgatgt ccacaatata tgacgcggta ttcagcgtca gactggcctt
     2401 gttaccatcc ggcaccccgt tgttccagtt ccctgcgtca ttccaaacgc cgccggacgg
     2461 gtttgtccac accgtcgtat cctgagccga accccatccg cacaccagca cagcgcaaaa
     2521 cgcccctacc atccagcccc ttctatcccg tatcttcatc gcttgtcctt tcatgatctc
     2581 aacgcctcaa tgaaccctga caaaccgagc ctgtctgacc gcaccttcca gggtctttcg
     2641 gacaatcctc cgtatcagcc ccttcccaca caacactatg ctacgtccga atcggaaaat
     2701 caaacctaga attcgaggtg gacttgacgg acgtgttccc gctgtcctcc ccgtaccctg
     2761 catgatccgc actttagcgt tcttaccatg aaacacataa aatttcatga aatcaagatg
     2821 gacgaaacga acgtccgctc gtccggacaa gcagacgctt gccacggttt ccgcgcgcgg
     2881 ccctttgtca agcaagtgca cgcttgacct cgacagtcag aatgaatacg gttttcgtta
     2941 tgtacgggtt ttaagacggg cacgataaca ctgttaaccg cgaggagcac atgagagcga
     3001 tacaggcaga catcacgacg ctacaggtcg acgcgatcgt caatgcggca aacacgacgc
     3061 tattgggcgg gggtggtgtt gacggcgcca tccatcgcgc ggccggtccc gatctcttgg
     3121 acgagtgccg gtcgctcggg gggtgcgcgg ttggcgacgc caagatcacg cgcggataca
     3181 agctacctgc gaaacacgtc atccacaccg tcggccccgt ctggcgcggt ggaaacacgg
     3241 gagaggagca acacctcgcc aactgttacc gccgatccct agaaatcgcg caagagcatg
     3301 gcgtgaaatc gattgctttc ccggcaatca gcaccggcgt ctacggctat cccattgaag
     3361 acgcgacacg agtcgccgtg gcaaccgtgc gcgcctatct gcaggagagc cagtcggcaa
     3421 tggatgtgat attctgctgc ttctctcagc gcgacctcgc ggtttacgat aaggaattgg
     3481 cgcgatagcg ctttgggaaa agagaggatg gcatgcgaga cgtttggatc gctttcggat
     3541 tgacattgtt cgcggggctg gcaaccggta tcggtagcat gatcgccttt ctcgccaagc
     3601 gtaccgatta ccggttcctc tctgtcgcga ccggtttttc ggcgggcgtg atgctctaca
     3661 tctctttcgt ggagatcttc gtcaagggca acgaggcgct ggtcgcacgt tacggcgacc
     3721 cgctcggttg ctgggtcaac gtggcttcgt tcttcggcgg catgctgttg atcgggctga
     3781 tcgatagcgt catcccgtcc gccgaaaacc cgcatgagat tcacgatgaa agtgagacgc
     3841 gcgtcctgcg cgaggggggc gaactggagc cgcacgatga tcccgcgcat ggcaagaagc
     3901 tgatgcgcgt gggggttttt accgccctcg ccatcacgat ccacaatttt ccggaaggac
     3961 tcgcgacctt cctcgcggcc ctgcaggatc ccaagctcgg tgccgcgatc gcgattgccg
     4021 tcgcgctaca caacatcccc gaagggatca gcgtctccgt gccgatctat tacgctacgg
     4081 gcaaccgccg caaggcgttc tgctactccc tgctgagcgg cctcgccgaa ccggtcggcg
     4141 cgggcgtcgc ctacttcgcg ctgcgtctct tcgcgggcgg cgaaaacggt gcgctcccgc
     4201 ctgaaatcat gggcatcctc tttggcggtg tggccggcat catggtctac atcagtctgg
     4261 acgagctgct gcccatcagc cgcgcctacg gcaaggggca cgacagcctt ttcggcgtcg
     4321 tgggcggcat gctggtgatg gccctcagcc tcctgctgat gcgatgagcc ggttggcggg
     4381 gcatgcgagt cgagcgcgtg atttttggtt tcagagtttc aaggtttctg gtttcatcca
     4441 aatcaggatc gggatcgcta tcgctttcgg gatcgattgc ttcaccacgg gacactttct
     4501 tggatttcag ggtttcaagg tttctggttt catgcatggc tcggtttttc tcacagaagg
     4561 tacaaagggc atgaaggttt ttgcggcatg gtttcactca gcattgccgg acacttgccg
     4621 gaacattacc gttttgtaat cggttgtgag cccgaagatc gcgggaatcc gtttccggcc
     4681 ggtcggcgcg aggcctcttt ttcaatctca tcaggagaga gtgcccgtgt ccagattctc
     4741 acctcgtcca tcaggccgat gaaatatccc ggcgtgctgg ttgatgcggg ctcgccaccc
     4801 caggcttccc gcccaaggat caggggttca tggttgacga tccggtcgga cgcattctgt
     4861 ttttcggcga ctttttttcc attcgcaaag agcgcaacgc ccttgccgcg ccgcacgacc
     4921 gcagcgacat gcgtccattg cccctccttc aatacgggct ccgatacgaa gttgaacgac
     4981 cattggtgtt cggtgtcggt ggcctcaaag ctgagcctgg caccgttctg agacaggatg
     5041 aacggcgcct cgcagccgga aaaccgcttg ccgaccaacc cgcgcctgcc ccgcagggtt
     5101 tctggcttga tccagagcga aaccgtcatg tccggtgcat tgaaaacgat cacctcattc
     5161 acgcacagcc aacctgtgcc gtcgaacctt cccgcacggc cccccacccc gttcgacacc
     5221 gttgtcgcgc cgccgttgag tctggcgcgg aaaccgttgc cggaaacatc ggcgacgttc
     5281 attcgttcgg tcttctcaaa gtcccagacg gcaaccggct ccgggcggtt gaaaaacggg
     5341 tgacggctaa gacgttcctg agcgatacgg cgttgcttca gatcatccat tatctcttcc
     5401 cggaccttct caaacgtgtt ggcgtgaagc caaatattcg ttgcgtgtac gctgacaaaa
     5461 atgccctgcg cggaattcgc cacatggttt ccttccacga tggcgtcgcg cgtcgcggcg
     5521 ccgacacgga tgtgcgcctg gctgtcgagc cggttgtgcc gcacgaccga ccccacgttc
     5581 agcggcccgg tgtaaggggc gcgtctgtac cctgtgattt cgagaagcgc atcggacgcc
     5641 ctattccagt ggtaatagtt gccctctttg atcgcgttgt cgcggaactc gcagaaccag
     5701 ttcggttgat agccgtgata ccataaccct atgccgcgga atccggacat gcgtgtgccc
     5761 gtgttccgca cgacgatatt ttccaccgcc gttccataaa actgcacagc cccacagtct
     5821 gagaattcat tcccgctgag aatataatgt ccttggaaca tcgtgacgca aatatccgaa
     5881 ttcgtgcccg gcaccacatc ccacggacgg tccacgatca cggactgctc accctgccct
     5941 gccacacggc ggtattgccc cgcgcctttg cccgcaagca caaacacccc tgcaccgcgc
     6001 cagtcctgcc cgcgccagtt cggcttgccg tcaagaacga gcgtcgttcc atgcgccgtt
     6061 acggcctttc cggaatatgc ctcgccaccg gcgtccgatg tcatcgcctc gcggtcccag
     6121 ccgtgcatcc gttgcaaacg gttatccgca taatacacat tttgcgagaa gcgtgagccg
     6181 tcgagacaat tcaagccgcc gccggtggac atcagatccg caccggtgat ctcgttgttc
     6241 tcaaaaatca gtccgtcgct tcccgagatg cagtaccagc cccagcgacc gttgaaaaaa
     6301 cggttgtcgg caacaagtcc gccacgtacg cggctcagaa acaacgtgcg cccggaaccg
     6361 tagaaatcac actccgtgat ctcaatgctg ctcccgccca tcctcaccgt atcgccgcct
     6421 cctgtggacc atttcatcgt ttcgcgaaaa cgcgcgtcca cctcgtcagg cgtcaaatgt
     6481 ccgcgatacg aactggcccg gacacgcaca cgccgcagaa acacattccc cgcgtcgggt
     6541 ttgtcgccta ggtctgcggc gatcacatga tggtggtggt gcgcgtagat cgtcaggtcc
     6601 tccagtccga atgaattcgt tccctgtacc agtgcccaag gcggattccg catatccgac
     6661 catgccagcg cgacccagtc cgaccgctcg ccccggagca ccgtgcgccg cgggatgttc
     6721 aaggtgtcgg aaaccgcata acggcccagc ggcaaataga caatgcctcc gccctgagac
     6781 tcggctgccg atagtgcgtt ttgaatcgca accgtgtcgt ccttcacgcc gtccgccagg
     6841 gcgccgaaat ttgtcacatt gaacacgttc tgcggccaga cctctttgtt tgtgaccgtc
     6901 aactccactg gtgcgctcca tcccgcacgg ccgccagcgc cattgtgggc aaacaggcgg
     6961 tacgatcctg acggcatgtc cttgggtaac acggcgcgcg ccgcatgacc gtccgtttcg
     7021 aacgcaaggg aaacgacaca cggtccttcg atcctgataa ccggtttcct gattccgtct
     7081 tttattcccc agccaaggtt cttcccgaaa aaccggatcc acccgcccgg ggtcacggca
     7141 ggaccgcact ccccctgcac ccaccagatc tgcgggcggt tcatgatatg ctcaaccgca
     7201 gcgccccctg cggccagacg aagcctgagc aaccccggct tgaaatccgc cggcacgatg
     7261 aattttgcag actgttcggc cgcctgaagc acatccaccg gggtgccgcc tctgggccaa
     7321 acgacgttct cttgcaacgg ctctttggcc cggccgtccg gtagccgcac aatctcgatc
     7381 gctgggtggt cgccaaggtt ttcgccgatc accatcgccg tctcacccgg acggaccggc
     7441 tcactagccc aaaaaatcac cggcctccgg tctttttcat cagcccgcac atcaagacag
     7501 gccgcaaaac tgagggccgt caccgaaaac agtgaaataa gaaccttctt catcctgctc
     7561 cttctcacgg gtgatcagaa atccgggcac ttgtgccgcc ggatactcat ctatttctct
     7621 ccgatgcaac agaaatcgcc cagcgttctc agatagatac ggttttccag aaagaccggc
     7681 gtggcgaaca cccccgacgg cagttcgttg gtcacgatcg cccccgccat ccgccctgtc
     7741 ggaaggatat atcccttgcc tttgcggtca aacaggtaga gcttgccgtc tgccagcgtc
     7801 ggcgaagaat agaacggcac tttgaagtcc tgttcccaga gcacgcgccc gtcaaccgta
     7861 tcgtaacacc gcaacagacc ttggtccgcg accgcataca gcaacccctc gtacagaatc
     7921 ggggaaggga cgtatccgac cttcgcgtca ctggaccagc ggatatggct tttcgtgacc
     7981 tctcctttgc cgtccgcgcg gatggcgagc aggatccggt tcggatatcc gccggtcgcg
     8041 tagaccgtat cccggtcaaa ggccgctgtc gcgacgcaga cttttttcgc cggtcctgca
     8101 cactcccaga gcagtgtccc gttctcggga tcgatgccgc gcgtgtatgt gccgcccagt
     8161 tgcagcaact gttcgcgccc ggcaatcacc gtaaccacgg ccggcgcgta gccttcgttg
     8221 acggcacgca gcttcgtgcg ccaaaccact tcgccggttg cccggtgaaa cgctgtcaga
     8281 taagaccctt tcgggccctc gaccggaatg atcactgctg acttgtaaat gttcggcgac
     8341 gcggaaaaac cttgaattgt gttgtacggg gcaactgtct ggcgccatcg gattttcccc
     8401 tccacatcca cggccaccat aacgaccgac gaatcggttt ggtacgccac aaagacccgc
     8461 tcgccgtcgc aggccggcgt agacgaggct ggcgaattgt cggcatgaac ttttgctgaa
     8521 gcccctatgt atagctcggc ctgccacacc gttttgccgg tattccggtc gaggcaaaag
     8581 agccagagcg ttgtctgccc ctgcgtcttg atccctgaag tgatgtaaat ccggttgccg
     8641 accacgcaag gggacgagtg cccgactccc ggtatcttta cctgccacag gatgttcgaa
     8701 ccggcgcccc atgccgtagg cggcttctgc ccggggggcg cgatgttgtc gaagttcggc
     8761 ccccgccacc acggccaatc gttttcagac tttttcacgg gagcaagcgt gtcccaaccc
     8821 gccttcggcg cgacatcggg cagccccccc tcctgcgcca tcaccgtcag cacactaagt
     8881 aaacccaact tgattgcgat gttttttttc acgctcgtat tactcctcgc cccgaagctt
     8941 tccggacaga atccctgatt ccaagcgaag ccaacgatcg aagccttcgc ccggcatccg
     9001 aagcgtcagg ggtaactaaa cataaaaaaa aggatcgtat cagtcaaccg caacctgacc
     9061 aggacgatcc ccagatttta tgttttattg atattcgtcc tgcgcgaatt tgacttccgc
     9121 ccggaacatc aatcttttat agtgatcatg taaaagaaat ctgtccccta tcacaccttc
     9181 gaaagtgtga taaaacgact ggagccaact gatgaaaccg ttgatgtcaa tccaactctt
     9241 tgcgtttttt gccgccgcgg tggcctacgg agaaagtgta aagccgaaaa tcgtcgacgc
     9301 tctccagccg atcacattga aacagtcgga gctttcaggc gagatcggca ggcggatcaa
     9361 cgatctgatc tatacgaact tcatggcgct tgatgtggac ggctttttcg ttgcgccgtt
     9421 ccggtcgcgc cccttcacaa acggctatca ctatgtcggc gttggaaaag tcatcgaggc
     9481 gggaagcctg ttcgccgcct atacgggcga cccgaaaatc gcggatcgga cgcgtcacct
     9541 gattgacggc atgatcggaa cgcgcgatgc cgacggatac atcggcgtgt tcaagcccga
     9601 gcccgatgat cgtcagaacc actggaactg ggcattgcat gagcaggagt atctgttgct
     9661 cggattaacc cgcaacgcgc ttctcacgca cgatcccaag atgttgcagg acgcgcaagc
     9721 gctcgcggat tatatgatcc gtaccttttc gaaagatccg catcccgagc aaatctgcac
     9781 cgccggactt cccgaagcct ttctcgtttt atataaggcc acaggcgaca cgcgctattt
     9841 cgattttgcc gccaatatcc gtcatggtaa tggtacggcc gagattaaat gcgcgtcact
     9901 gcgggattgg cgccagactt tcgagacccg ccccgcgcat gtctacgtga tgcttgcgcg
     9961 gtgctacgcg caggcggaac tctaccgtta cgacggggtg gcgtcactca tggatatgtc
    10021 gaagtatatg cgccacgaat tgctgcgccc cggcggcggg ctcaatgtgg tcggctcggc
    10081 ttctgacggc gaacactttt cctatacgca gaacggtgcg ggggctatcg gcgaatcgtg
    10141 cgtgacagcg tatttgctgc gctggctgga cagcctgatg cgcctggagg gagatctccg
    10201 gtacggcgat ctgatggagc gtacggttta caacgcgctt tttgcggcgc aagaccctgc
    10261 gggaaggaaa ctccggtact tcacgccgtt taccggcgtc cgagcctatt atgggcaaga
    10321 tggtttttgt tgtccgggta attatcgccg catcgtggcg gagttgcctc agaaagttta
    10381 ttaccggtgc tcggacggcg cattgtcggt caacctgttc acggcctcga aagtgacggt
    10441 gccggtctct cccgatgcga ccgtgacgct tgcacagaaa acggattatc cgaataccgg
    10501 gcatgtggtg ttccgcgtga cgccgtcaaa agaaaccgtg ttcaccctgc gtctgcgaat
    10561 tcctcgctgg tgccgcaccg caaccgtcac agtcaacaac gagtctccgc tttccgtctt
    10621 gccgggtgcc aaggctttcg aactgcgccg tgtgtggaaa accggcgaca cggtgacgct
    10681 ggatatgccg atggcgtggc ggtttgtcaa aggccaccag ttacaagagg ggaaagcggt
    10741 gctgatgcgt ggtccaattg tgtattgcct aggcacggca caaaatagcg aggtactgga
    10801 aaaatacccg aatttttctg gagtggtgat tgatccaacc tgtatcggag agatagaaaa
    10861 cgacacgtcg attcgccctg acgggaaaaa ggtcaaggct caggcaaggg ttgaagcacc
    10921 tggagcgggg cgaaaaggtt cgccgcacgt gacgttgacc tttacggagt tcatcgatcc
    10981 gacgggcatc gccacctatg tgcatctgct tgacctcaac aaagccgagg aagacgaact
    11041 cgtgtcggac tgttttgcca aaccgtgatt gttaaaagga gccgggatgc cccgcagatc
    11101 gtaccttatg tccgtagtcg cactgttgct cgcaaccata acgcggtgcg cggaaacccc
    11161 aacttggcgt ccggaaaccc gttggcgagg cttcaacctt ctcgaactgt tctcgtaccg
    11221 gggacagccg gccgcatttc gggaacatga ctttcgcctg atcagcgagt tggggttcaa
    11281 tttcgttcgt ctgcccctgg attaccgctt ctggatttgc gacggaaact gggaagcgtt
    11341 cgacgactct aaactcgcgc ccatcgatca ggcgatcgcc tggggcaaga agtacggcat
    11401 ccatgtgcag ctctgcttcc accgcgcgcc gggttacacc gtggcccaac cttcggaacc
    11461 gcacaacttg ttttccgatg aacacgcgct gcgcgtctgt tgccatcact ggagccggtt
    11521 cgcgcggcga tatcaaggga tcccctcgca tgcagtgagc ttcaaccttt tcaatgagcc
    11581 cggcgaggtt gaggaagcgg cctatgcgaa ggtggcgggt gcgctggtcg ccgccatccg
    11641 gacggaggat ccggaccgct tcattgtggc ggacggcatc gcatggggcg gacgccccgc
    11701 ccagtcgctt ttcaaactcg ggatcgggca ggcgatgcgc ggctacaagc ccatgtcgat
    11761 ctcgcactat ctggcgagtt gggtcggcac accatctgcg gagccgatat ggccgccgcc
    11821 gcaggccgtc agtccacttt atgggccgat caaagctccg ctgaatgtgc cgctggtaat
    11881 ggagcgcgtc cctgcaggaa ccctgaccct gcggctcggg atggtgtcgg gcagagtttg
    11941 cctgcgcgtg accgcagatg aggccgtggt gctgaaatgt gacctagacc cgaaaccggg
    12001 caccgcaggc tggacaaatg tggtgtacaa aagcgaatgg aaaatttcgc aggggcgatg
    12061 tctttccgtg ttgagcgtgg atcttccgaa gggggcgcag cggctctccg tcagcattcc
    12121 ccaaggcgat tgggcgcaac tctcccaagt caccttgacc ggaaagtcgg gccaggcgga
    12181 atcgctgtgg tttgagcaaa cgtggggcaa gaccaaccgc gtgttccgtt ttatgggatt
    12241 cggcgcatgc cccgccttcc aatcaggcgc ggaggcttgc gacgggctta cctatctccg
    12301 tcagaacgtg ataaaccctt ggcaacccgc ctttgatgca gggattttca cgatggtcgg
    12361 cgagttcggc gccttcaagc atacgcccca tccgctagtg ctgaagtgga tggaggacaa
    12421 cctgaaactc tggaaggaag caggactggg ctgggcgctg tggaatttca gagggacgtt
    12481 cggcattctc gacagcgagc gcgccgacgt gcgttacgaa gagttccacg gtcacaaact
    12541 agaccgcagg atgctggacc tgctgcagaa atactgaccg ttccgttagc gccgacatgc
    12601 agagcacaaa gggggaatgg attgcgggct ccgcccgcgt tagttcccaa gttcgtgggc
    12661 cgggttgtgt cccgtggtga aacaagcgat cccgatcccg atagcgatcc cgaccccgat
    12721 ttggatgaaa ccctgaaacc ttgaaaccca agaaagtgtc ccgtggtgaa acaatcgatc
    12781 ccgatagcga tcccgatagc gatagcgatt tggatgaaac catgaaacct
//
LOCUS       DAOSLE010000077        12753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_4974_length_12753_cov_7.447236, whole genome shotgun sequence.
ACCESSION   DAOSLE010000077 DAOSLE010000000
VERSION     DAOSLE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4974_length_12753_cov_7.447236"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..82)
                     /locus_tag="PK576_06590"
     tRNA            complement(6..82)
                     /locus_tag="PK576_06590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Pro,seq:cgg)
     gene            275..1231
                     /locus_tag="PK576_06595"
     CDS             275..1231
                     /locus_tag="PK576_06595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOE61297.1"
                     /translation="MKRVLLMLLAMGCCIGDVWAQGAKTTRRAPAKKTAAPAQPQKVK
                     GCISRSPYIGAVILDADSGAVLFEERANVPGYPASTLKLMTLLVIQEKIEAGQIRLDD
                     QVKVSVEAYKTGGSQVYLDPKETFTVEEMLYAMIIQSANDAAVALAEHVAGSKEAFLV
                     LMNARAEALGMRDTRFASVHGLPPSAGQRHDVSTARDMAILARELCRYPAIFAYTSAT
                     YRQLRANTPNPFDMRTHNPFLKEGVEGCDGFKTGYTATAGWSIVVTAKRNGRRVIVVV
                     LGSEGRKQRDAEARALLNRAFTLLQPEGAPAQAVPHPASVAR"
     gene            complement(1237..1797)
                     /gene="thpR"
                     /locus_tag="PK576_06600"
     CDS             complement(1237..1797)
                     /gene="thpR"
                     /locus_tag="PK576_06600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOE61298.1"
                     /translation="MRLFLALSLPDSVASRLTDAVRRLEPEATDVAWCRRDQFHVTLA
                     FLGDTAPAILPHLTAACERVCSACPSFSCRAYGFGFFGSKRSPRTLWAGVDPIPELMA
                     LQEAIWQTLPKFGFADNETHFCPHITLGRCRKGARNLRLIEAMDAEDQTDFGSWRVTR
                     VTLYESRPSPRGPIYRAASLFPLATN"
     gene            complement(1938..2732)
                     /locus_tag="PK576_06605"
     CDS             complement(1938..2732)
                     /locus_tag="PK576_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOE61299.1"
                     /translation="MIRPAAHKAIVILDFDGTLYRGLCPALCRGIANADLLVALCLIN
                     LSRPRYFFRLLREATRLQKLQQRLARAYTEKRLSLSSADGRLIRFFTHRIMMCCTPTV
                     LDQAAALVSRLCYPSAWRALSAVRGDCDFAVVSKSFDFLLEKVRQRAATRGVHLQVVG
                     TRVRCGGAGWRIEVPMTRAEKALQVRELLMGRAYDRAVVIGDTEDDIGMRDAAVKVLG
                     AESVTFIALNVKDNEIKSAADVSCPSWRQVGAFLRGWVSSVSTGTS"
     gene            complement(2729..3538)
                     /locus_tag="PK576_06610"
     CDS             complement(2729..3538)
                     /locus_tag="PK576_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61300.1"
                     /translation="MSDGRLPEKAELTVVHLTDLHLDTQGKVTHTPWTHRIAVGGYRL
                     HRPCTGRAFEYLEQAVSQIRDTVKPDVVVITGDVVNRGDDIEALRRGVSILSRLQCPV
                     VVVQGDHDIPRRKENGTVWNDLFDTTHGCTLVNNTRFFFLPFVKDETAFADTLQAMRT
                     VSRRDKIHFFCLHRMLWAPRLMDALARRYHGCSVLDPDREKIVEAMDTSGARWMVLCG
                     HSHTDYCRTRGNITQLCAPSLAEHPHAFRVIKIKDGQVFYKLVRLDKGKIK"
     gene            4266..6389
                     /locus_tag="PK576_06615"
     CDS             4266..6389
                     /locus_tag="PK576_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOE61301.1"
                     /translation="MCKSFLKRLLPVVVCGLSACGHAAPTKTLVAWVYLDNTTQQGGS
                     ALTIQRGKQFDAIVFGEKAAGKWMAGSNYFRRTQDDQQSNAAETAAADTLIQMAIVYT
                     EQRITIWRNGEMYADYAAANIDLLSAPDNIAVFGLRHEGATTGQTLAGKIEDARIYDR
                     ALTQAEISALRPDQPSTIKPYAWWTFEPGQETDRAGRFAINVLSGGAKIEDGMLKLQS
                     GGKLVAHMPASSVPPEQLAMPENPPATWLTYHLAHPGPSGARPGDPNPAFYYRGRYHL
                     HYIYNHKDGFAYAHVSSTDMVHWKWHPTTITPKTMGHGMFSGTGFFTRNGTPAMIYHG
                     HGSGRNQLAFALDDNLEHWSQPQAIEPFTAAGEKPKMRHWDPDCWFMDNAFYALSGGE
                     NPKLMKSADLRNWLYLGDLFHESFPTDLGVAKGEDVSCANMFRLGNKWMLLCISHGLG
                     TRYYLGDFIDEKYLPSHHGMMNWAKWDCFAPESLLTPDGRRVMWAWCTPWTNTMQRVG
                     RSKNFDALLNQAVFQQGIQSLPRELSLPEDGVLRIKPLRELEALRYDRQEQRKITVPG
                     EAQQLLSGISGDTLELEVVFSARQAQKFGINLLCDAKGDNGFKISCDAVSGNLNVGYV
                     TPSFQVKASEDLTLRLFIDKSMIEVFVNERHAVVAWHEYPPDNRHISLFAKGGNLLVK
                     KMTAWKLKSIYAGNCVFDPAASKKQ"
     gene            6594..7076
                     /locus_tag="PK576_06620"
     CDS             6594..7076
                     /locus_tag="PK576_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOE61302.1"
                     /translation="MQGMENTIEARVVEICRERKQTLVTAESCTGGLVACRITSVPGA
                     SDIYLGSVVSYANAVKCGMLGVTPEILARDGAVSAVCAEAMACGVRERLGADLAVAVT
                     GVAGPDGGTLQKPVGLVFLAVATTAGVRSEACRFGGNRAEVRMRAADRALTLVLESLG
                     "
     gene            7207..8421
                     /gene="argJ"
                     /locus_tag="PK576_06625"
     CDS             7207..8421
                     /gene="argJ"
                     /locus_tag="PK576_06625"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOE61303.1"
                     /translation="MKCFEFVEGGVTAARGFRACGIEAGIKDAGRKDLAMLVADAPAA
                     VAAVYTTNKVAAAPVRIDRERTPAGQAQAVVINSGCANACTGETGLQNAREMARLTGE
                     ALGIDEQLVLVCSTGVIGVHLPMERIAKGIKKAVGALSSTGGSDAAYAIMTTDTVDKQ
                     VAVELDVDGKTVMIGGMCKGSGMIEPDMATMLGFITTDADVHPKALDQALREAVEVSF
                     NRVVVDGDRSTNDTVILMASGEAGNETLTPYHPQWQYFVDALMTVCLELGKKMVLDGE
                     GATKFVTVRVRGARTEQDAQAAARAVAKSALVKTSWFGLDPNWGRVIAAVGYSGAEVD
                     DQKAQVYYGDICAYDQGHVADKALLKAMQDVMRKRAFDVTVDLHLGEGEDTIYTCDLS
                     CDYVKINAEYTT"
     gene            8564..9103
                     /locus_tag="PK576_06630"
     CDS             8564..9103
                     /locus_tag="PK576_06630"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE61304.1"
                     /translation="MTQKAFYDWQTAGGGGDVMRLVSVLERLDVPWCVIGGIAVNHWV
                     EEPMVTADVDLVIALDRVEEAVCALEKEGFKAERFPWSINLKGRSKVSVQISTEERYR
                     QFPARAVAADVWGMLMRVASLEDTLAGKLAAYVDESRRPSKRQKDWLDILRLVESHPE
                     LADRLPDEVRTKLKEVGPC"
     gene            9097..9519
                     /locus_tag="PK576_06635"
     CDS             9097..9519
                     /locus_tag="PK576_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61305.1"
                     /translation="MLKNAQRSTFNAQVSVQNLECGDKSRSVRGSRHRFLSALRAEPL
                     CTLAQKAASRGIPLAAALQIFSPDLRSRRYTVKISASPRLCVIKKCNAICGRANKPDV
                     SQVFAASTTIIDDVKAEIKRQEKRQKPDGVDVREEAVK"
     gene            9516..10202
                     /locus_tag="PK576_06640"
     CDS             9516..10202
                     /locus_tag="PK576_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61306.1"
                     /translation="MKKERPCLIVCALFAAGVCAQEAPVAARNTPGRYQAMIGGDNTV
                     YVIDTETGAMLSKREPGFGWEIESDGFSNTVAAVRKKQDDARKARKALADLVASTAET
                     FPELSLEDKVKAAADIFVVKVQMCTVGNRPMRPRCFVAEYLRKVVGGPKRVYDVGGTF
                     PGFNVDLESGAKEDIIVLCFEVRGNGTNAFVCIPTVPVKLWECATYMIVLDASPTIVE
                     DAKEVLAKKE"
     gene            10204..11082
                     /gene="argB"
                     /locus_tag="PK576_06645"
     CDS             10204..11082
                     /gene="argB"
                     /locus_tag="PK576_06645"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOE61307.1"
                     /translation="MKQFIEKAAVLTEALPYIQDFKGSVVLVKVGGSVMEVEENLDRL
                     LTDIAFMNAVGMKTVLVHGGGKAISRGMEAAGVTPQFVQGLRVTTEEAMRVVERVIKD
                     EVNAKVVRMLQRHFANARPLHGDWIFGVVKKTGTDPVSGAELDWGFVGEPVSVDTRPV
                     AEMLDAGIVPVVTPLGTGPDGKVYNVNADTAAAALAKAMKVRKLAFVSDVPGLLMDMN
                     DPASLLATLRIGSVRKLKETGVVGGGMLPKLDSCVEAIQAGVGKVHLVDGRMPHSLLL
                     EIFTKKGVGTEIIADE"
     gene            11075..12277
                     /locus_tag="PK576_06650"
     CDS             11075..12277
                     /locus_tag="PK576_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOE61308.1"
                     /translation="MSKSDDIAALFNQYVMHTYAPTVTLTSGSGCKVRDPDGMMYLDF
                     TAGIAVHNVGHCHPKVVKAVQDQVATLGHCSNLFYNASQALLAQKLSKLSLNGKCFFC
                     NSGAEANEAMIKLARLWGNAKGKYEIICMQNSFHGRTLATVAATGQAKIQKGFDPLPI
                     GFTFAEFNNLDSVKAAVSERTVAVMLEAVQGEGGVMPATEEFMVGVRKLCDEKGLLML
                     CDEVQCGMGRTGKWFAWQHFPVKPDAFTLAKSLADGIPMGALVASPALSDLFQPGHHA
                     STFGGNPVSSAAALAVMEVIEEEALVTQAENAGTLFVEGLAMFVEKYDQVLEVRGKGL
                     MVGMVVEGSAKEVVDACREMGLLCCVAGEHVVRFLPPLNVKDSDLEEALEMIGDALDQ
                     LFGGAEEA"
     gene            12363..>12753
                     /locus_tag="PK576_06655"
     CDS             12363..>12753
                     /locus_tag="PK576_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOE61309.1"
                     /translation="MKKLIGMSLLAMVPLLRADVQVIETVCPNGAKARYFEEMVPMPD
                     GVKLYTYGAVPPDGMKCPIVIQRNPYVKDERVKPETFAGQRWTPDSYVWITQHCRGCG
                     RSEGDWIPYATERADGLAFLEWIRKLPF"
BASE COUNT         2718 a         3316 c         4104 g         2615 t
ORIGIN      
        1 aaaattggtc ggagcggagg gatttgaacc ctcgaccttt tggtcccgaa ccaaacgcgc
       61 taccaggctg cgctacgctc cgagaaaaac cgtaaaccgg cgcatacatt agccgaaccg
      121 caggtaaagt caaatcgaaa aaggagcgtg gaaacgattt tttgtaccga ggtctttcca
      181 atcggcggca aacatgatta actacgcgtc aattgtttga cgccgcaggg tatgcggggt
      241 cttggccgca ggcgtaaaga aaaaaggggg aagggtgaag cgggttctgc tcatgttgtt
      301 ggctatgggg tgttgcatcg gggacgtgtg ggcgcagggc gcaaaaacga caagacgcgc
      361 acccgccaaa aaaacggccg cgcccgccca acctcagaaa gtcaagggat gcatttcccg
      421 gtcgccctat atcggtgccg tgattttgga cgcggattcc ggagcggtgt tatttgagga
      481 gcgcgccaat gttccgggat acccggcgag cacactgaaa ctgatgacct tgctggtgat
      541 tcaggagaag atcgaggcgg ggcagatccg gttggatgat caggtgaagg tttcggtcga
      601 ggcgtacaaa accggcggtt cgcaggttta tctcgatccc aaggagacgt tcacggtgga
      661 ggagatgctg tacgccatga tcatccaatc cgccaacgat gcggcagttg cgctggccga
      721 acacgttgcg ggtagcaagg aggcgtttct cgtgctgatg aatgcccgtg cggaagcgct
      781 cgggatgcgg gatacgcgat tcgcctccgt gcacggactg ccgccgtccg cagggcagcg
      841 gcacgatgtc tcgaccgccc gtgatatggc catcctcgcg cgggaactgt gccggtatcc
      901 ggcgatcttc gcctacacgt cggcgaccta ccggcaactt cgcgccaata cgcctaaccc
      961 gtttgacatg cgcacgcaca acccgtttct caaagagggt gtggagggat gcgacgggtt
     1021 caagaccgga tacaccgcga ccgcgggatg gtcgattgtg gtcacggcga agcggaacgg
     1081 aagacgcgtg attgtggtgg tgctgggtag cgagggacgt aaacagcggg atgccgaggc
     1141 ccgcgcgttg ctgaaccggg ctttcacgct gttgcagccg gagggagccc cggcgcaagc
     1201 cgtgccgcat cccgcctcag tcgcgcgctg acctcttcag tttgtcgcga gcgggaacag
     1261 acttgcggca cggtagatcg ggccgcgggg cgagggacgg ctctcgtaca gcgtgacgcg
     1321 tgtgacccgc cacgagccaa aatccgtttg atcttcggca tccatggctt cgatcaggcg
     1381 caggttgcgc gcgcctttgc ggcaacgtcc cagtgtgatg tgagggcaga agtgggtttc
     1441 gttatccgcg aaaccgaatt tcggcagcgt ctgccagatt gcctcctgca gcgccattag
     1501 ctccggtatc ggatcgaccc cggcccaaag ggttcggggg gaacgcttag aaccgaagaa
     1561 cccgaaaccg tacgcacggc aggagaagga cggacatgcg gagcagacgc gttcgcaggc
     1621 ggcagtcagg tgcggaagaa tagccggtgc ggtgtcgccc agaaaggcaa gggtcacatg
     1681 gaactggtct ctgcggcacc aggccacatc cgtagcctca ggttccaacc gacgcacggc
     1741 atcggtcagg cgggaggcaa cggagtcggg cagggacaag gcaaggaaaa ggcgcatatg
     1801 tcgtagaata catcaagtcc gccggggttt caatgacgac cttgtcgtac cgatgatcca
     1861 gaatgaagtt ccgtattgtg ttaaaaaaag agaacagtac ttttataccc gccgccgttg
     1921 caaactactg cgtggtctca actggttcca gtcgacaccg atgacaccca acccctaaga
     1981 aatgccccga cctgtctcca agaaggacag gagacatcgg cagcgctctt tatctcatta
     2041 tccttcacgt tcaaagcgat aaacgtaacg ctctcggcac cgagaacctt tacggcggca
     2101 tctcgcatgc cgatgtcatc ttctgtgtca ccgatcacca ctgcccggtc atatgctctg
     2161 cccatcaaca attcgcgcac ctgcaaagcc ttctccgcgc gagtcatggg cacctcaatg
     2221 cgccagcctg caccgccgca tcgcacacgc gtgccgacaa cttgcagatg cacaccacgt
     2281 gtcgccgctc gctgccggac tttctccaga agaaaatcga aggatttact gaccaccgca
     2341 aaatcgcaat cgccacgaac agcactcaac gcacgccaag ccgacgggta acacaaccga
     2401 gataccagcg cggcagcctg atccagtacg gtaggcgtac aacacatcat tatccggtgt
     2461 gtgaaaaagc gtatcagtcg tccatctgca ctggaaaggc tgagtcgctt ttccgtgtag
     2521 gcacgggcta gccgctgttg taacttctgg agccgcgtgg cttcacgcaa gagccgaaag
     2581 aagtagcggg ggcgcgacag attgatcagg cagagcgcaa ccagcaggtc ggcattcgcg
     2641 atgccgcgac acagcgcggg acacaacccg cggtagaggg tgccgtcaaa atccagaatg
     2701 acgatggctt tatgcgctgc gggtcttatc acttgatctt tcccttatcc agtcgcacaa
     2761 gcttgtaaaa gacctgtccg tctttgatct ttataacgcg gaaggcatgc ggatgttcag
     2821 ccaagctggg cgcgcacagt tgcgtgatgt ttccccgcgt ccggcagtaa tctgtgtggg
     2881 agtgcccgca gagcaccatc catcgcgcac cggaggtgtc catcgcttcg acgatttttt
     2941 cccggtccgg atcaagaacg gagcagccgt gataccgtct ggccaaagca tccatcagac
     3001 gcggtgccca cagcattctg tgcagacaga aaaaatgaat tttgtcccga cgggaaacgg
     3061 tacgcatggc ttgcagagta tcggcgaagg cggtttcgtc tttcacgaac ggcagaaaga
     3121 aaaagcgcgt gttgttgaca agtgtgcaac cgtgcgtcgt atcgaacaag tcgttccaga
     3181 ccgtgccatt ctccttccgt ctcggaatgt catggtcgcc ttgaacgacg acaaccggac
     3241 attgaagacg gctcaaaatg gaaacgccgc gtcttaacgc ctcgatgtcg tctccgcggt
     3301 tcacgacatc gccggtgatc acgacaacat ccggtttgac cgtgtcccga atttgtgaga
     3361 ccgcctgctc cagatactca aaggctcttc cggtacaggg ccgatgcagc cggtaaccgc
     3421 ccacagcaat cctgtgtgtc cacggtgtat gcgtgacttt gccctgtgtg tccagatgca
     3481 ggtcagtcag atgaacgacc gtcaactcgg ccttttcagg cagacgtccg tcactcatgc
     3541 ttgagtgccc aaaacacact ccaagaaaga ggatgacagc tccccagaaa agccactgct
     3601 ttttgttgtt tcttcgtgtt gacattttcg gggtatagtg cttcacaatg cgggaacata
     3661 cgcttcgtcg cttacgctta gcccacatgc tcattccagc cgcctcgtcg ctgttacttt
     3721 accggaacag ccgtctaaat gcaatcgacc aaagtggtcg tcgaacgtcc tcgcgggagg
     3781 ctagtcgaat tcccttcgca tttccgcgta cgtgaacgtg tgcgacggca tccgaggata
     3841 tgcgtttgct tgcccgtgag gacggttcgc cgaaccgtcc gcggacgccc cggggaggtg
     3901 tccctgccgg agcccggcac ggttggtttc ggcggcccgg ctccgcgccg gtggcgccgt
     3961 agctgtggac gtcccgccgt actggcagga accgtccgca gttcgcgggg acgcccgtgg
     4021 ggataataaa tgtccacgtt atcggtggaa agggcgagtt ccggaacctg agttttcaac
     4081 gcgcagtgac ttggggagat gtccgccgga cttgcggaga cgcggcatgg agagtatagt
     4141 accgatgcga tgcagggagt ggaaaacacg atcgaggctt tgaatggcaa agcatttttc
     4201 gcgctatctc catccagcac attcacacac ttaggatgcg ggctctgccc gcgttaggag
     4261 tcgccatgtg caaatctttt ttaaaacgct tgctgccggt tgttgtttgt ggtctgtccg
     4321 catgcgggca tgccgctcca accaagactc tggtggcctg ggtctacctt gacaatacca
     4381 ctcagcaagg cggcagcgcg ttgacgatcc agcgtggcaa acagtttgat gccatcgtgt
     4441 ttggcgagaa ggcggcaggc aaatggatgg cgggcagcaa ctatttccgg cgtacgcagg
     4501 acgaccagca aagcaatgcc gcggaaacgg ccgcagccga caccttgatc caaatggcca
     4561 tcgtctatac cgagcagcgc ataaccatct ggcgcaatgg cgagatgtac gcggattacg
     4621 cggcggccaa catcgatctg ctcagcgccc ccgacaatat cgcggttttc ggattgcggc
     4681 acgaaggcgc caccactggg caaacgctag ccggcaaaat cgaagacgca cgtatctatg
     4741 accgcgccct gacacaggct gaaatcagcg cattgcgccc cgaccagccc tcgacgatca
     4801 aaccctatgc ctggtggacc tttgagccgg ggcaggaaac cgaccgggcg ggacgcttcg
     4861 caataaacgt tttgtccggc ggcgcaaaaa tcgaggacgg catgctgaaa ctgcaatccg
     4921 gcggcaaact ggtggctcat atgccagcca gctccgttcc tcccgaacaa ctcgccatgc
     4981 cggaaaaccc tcccgccact tggctgactt accatctggc ccacccggga cccagcggtg
     5041 cgcgtccggg cgatcccaat ccggctttct attacagagg cagatatcat ctgcactaca
     5101 tctacaacca taaagacggc ttcgcctacg cgcatgtttc cagtacggat atggtgcact
     5161 ggaaatggca tccaaccaca atcaccccaa aaacgatggg gcacggcatg ttcagcggca
     5221 ccggtttctt cacccgaaat ggaacaccgg ccatgatcta ccacggtcat ggctccggcc
     5281 gcaaccagct tgcgtttgca ctggacgaca acttggagca ctggtcccag ccccaggcga
     5341 tcgagccttt taccgctgcc ggtgaaaagc cgaagatgcg gcattgggat ccggactgct
     5401 ggttcatgga caatgccttt tatgcattga gcggcggcga gaacccgaaa ctgatgaaat
     5461 ctgccgacct ccggaactgg ttgtaccttg gcgatctctt ccatgaatcg ttccccaccg
     5521 atctgggtgt cgccaaaggc gaggatgtct catgcgccaa catgttccgg ttaggcaaca
     5581 agtggatgtt gctctgcatc agccacggtc tgggtacccg ctactatctg ggcgacttca
     5641 tcgacgagaa gtacctgccc agccatcacg gaatgatgaa ctgggccaaa tgggactgct
     5701 tcgctccgga gtcgctcttg accccagacg ggcgtcgtgt catgtgggcc tggtgcacac
     5761 cctggacgaa tactatgcag cgtgtcgggc gctccaaaaa tttcgatgcg ctgctgaatc
     5821 aagccgtctt tcagcagggc atccagtcat tgccgcgcga actgagcctc ccggaagacg
     5881 gtgttctgcg cattaaacca ttgcgcgaac tggaggcgtt gcggtatgac cggcaagagc
     5941 agagaaagat caccgtgccc ggagaggcgc aacagttgct gtcggggatc agcggagaca
     6001 cgttggaact ggaggtggtt ttttccgcgc ggcaagcaca gaagttcggc atcaaccttt
     6061 tgtgcgacgc caagggtgat aatggattta agatttcttg tgacgctgtc agcggcaatc
     6121 tgaacgttgg ttatgttacc ccgtcttttc aggtcaaagc ctccgaggac ctgaccctgc
     6181 ggctcttcat cgacaaaagc atgatcgagg tttttgtcaa cgaacgccac gccgtcgtgg
     6241 catggcatga gtatccgccc gacaaccggc atatcagcct gtttgccaaa ggcggcaacc
     6301 tgcttgttaa aaaaatgacc gcctggaaac tcaagtcgat ttatgcgggt aattgcgtgt
     6361 ttgacccggc cgcctcaaaa aaacagtgat tgaaatcacg tagcgatatg gattccggtg
     6421 tcagctggtt tgggctttgg gatcgaagtc gacacgcttg acttgacact gcgcggttac
     6481 cgcgtgcgac cgatctgcac gtcacgtccg ggcgcaacgg tcaacggcca gtgactcggg
     6541 gggtgtccac cggacttgcg gaggcgcggt acggggagta tagtacagat gtgatgcaag
     6601 gaatggaaaa cacgatcgag gcgagggtgg tggagatttg ccgggagcgg aaacaaacgc
     6661 tggttacggc ggagtcctgt acgggcggtc tggtggcctg tcggatcacg tccgtaccgg
     6721 gggcttcgga tatttatctc ggtagcgttg tgagttacgc caatgcggtc aaatgcggca
     6781 tgttgggcgt cacgccggag attctggcgc gggacggtgc ggtcagcgcg gtctgtgcgg
     6841 aggccatggc ctgcggagtg cgcgaacggc tgggtgccga tctggcagtc gcggtcacgg
     6901 gagttgcggg gccggacggc ggcacgcttc aaaagccggt cgggctggtg tttttagcgg
     6961 tggcaaccac tgcgggcgtg cgctccgagg cctgtcggtt tggcggtaac cgcgcggagg
     7021 tgaggatgcg ggcagcggat cgggccctga ctctggttct ggagagtttg ggttgaacgg
     7081 atgggattgc gctgttggtc gtcccggaaa ggggggctcc tgtttttcat cgacttgggc
     7141 aataaaatat atcaaactac attatttaca cgggattctc ttttttcccc gaagcggaat
     7201 gcggttatga agtgttttga atttgttgaa ggcggggtta cggcggcgcg cgggtttcgc
     7261 gcgtgcggga tcgaggcggg catcaaggac gccgggcgta aggacttggc gatgctggtg
     7321 gccgacgcgc cagcggcggt ggcggccgta tacacgacca ataaggttgc ggcggcgccg
     7381 gttcggatcg accgggagcg gacgcctgcc gggcaggctc aggcggtagt catcaacagc
     7441 ggctgcgcga acgcctgtac cggcgagacc gggttgcaga acgcacgcga aatggcccgc
     7501 ctgaccggtg aggcgctggg catcgacgag cagcttgtgc tggtttgttc aaccggcgtg
     7561 atcggcgtgc acctgccgat ggagcgtatc gcgaagggca tcaagaaggc ggtcggcgcc
     7621 ttgagttcca caggaggcag tgatgccgcg tatgcgatta tgacaaccga cactgtggac
     7681 aagcaggtgg cggtggaact tgatgttgac ggcaagacgg tgatgatcgg cggcatgtgc
     7741 aaaggatccg gcatgatcga gcccgacatg gcgacgatgt tggggttcat cacgacggat
     7801 gccgatgtgc atccgaaagc tttggatcag gctttgcgcg aggcggtgga ggtaagtttc
     7861 aaccgggttg tggtggacgg cgaccgcagc accaacgaca cggtgatcct gatggccagc
     7921 ggcgaggctg gcaatgagac gctgacgccg taccatccgc agtggcagta tttcgtggac
     7981 gcactgatga cggtctgtct ggaactcggt aagaagatgg tgctggacgg cgagggcgcg
     8041 accaagttcg tgacggtgcg cgtgcgcgga gcgcggaccg agcaggacgc acaggcggcg
     8101 gcgcgcgcgg tggccaagtc ggcgttggtc aaaacctcgt ggtttgggct cgaccccaac
     8161 tgggggcgtg taatcgcggc ggtcggctat tccggcgcgg aggtcgatga ccagaaggca
     8221 caggtctatt acggcgatat ctgcgcgtat gaccaagggc atgtggcgga taaggcactg
     8281 ctcaaggcca tgcaggatgt gatgcgcaag cgggcctttg acgtgacggt agacctgcat
     8341 ctcggtgagg gtgaggatac gatttatacg tgcgatctct cgtgtgatta cgtgaagatc
     8401 aacgccgaat acacgactta agctgagcgg aagcatggcg accccgggat tcggacgaca
     8461 acgagggata agtttagcca ggattacagg atttacagga tggggtttct gatcatggga
     8521 attctgtaat cttgtcaatt tatcggtaag gcggataggg agcatgacgc agaaggcgtt
     8581 ttacgattgg cagacagcgg gcggaggcgg ggacgtgatg cgtctcgtct ccgtgctcga
     8641 acggctggat gtgccttggt gcgtgatcgg cggcatcgcg gtcaaccact gggtggaaga
     8701 gccgatggtg acggccgatg tcgatctggt gattgctctg gatcgcgtcg aggaggcggt
     8761 ctgcgcgttg gagaaagagg gatttaaagc ggagcgtttt ccttggtcga tcaatttgaa
     8821 aggcagatcg aaggtcagtg tccagatttc cactgaagaa cgctaccgac aatttccggc
     8881 gcgtgctgtg gctgcggatg tgtggggcat gttgatgcgc gtggcttcgc tggaagatac
     8941 gctggcgggt aagctagccg cgtatgtcga tgaaagtcgg cggcccagta agcggcagaa
     9001 ggattggctg gatatcctgc gcttggtcga gagccatccg gaacttgcgg ataggttgcc
     9061 ggacgaggtc agaacaaaac tgaaggaggt tgggccatgt taaaaaacgc tcaacgttca
     9121 acgttcaacg ctcaagtaag tgtgcaaaat ttggagtgcg gtgacaagag tcgctctgtg
     9181 cgcgggtcgc ggcaccgctt tctctccgca ctgcgcgcgg agccactctg tactcttgct
     9241 caaaaagcgg cgtcgcgcgg aataccgctt gccgccgcac tccaaatttt ctcgccagac
     9301 ttgcggtcac gccgctacac cgtaaaaatc tctgcgtctc cgcgtctctg cgtcatcaag
     9361 aagtgcaatg ccatttgtgg ccgggctaat aaacctgatg ttagtcaggt tttcgccgcg
     9421 tcgacaacga tcattgacga cgtgaaagcg gagatcaagc ggcaagagaa gaggcaaaag
     9481 cctgacggtg ttgatgtgcg cgaggaggct gtgaaatgaa aaaagaacgg ccctgtttga
     9541 tagtgtgtgc tttgtttgcg gcgggggtgt gtgcgcaaga ggctccggtt gccgcgcgga
     9601 atacccccgg tcgttatcag gctatgatcg gaggggataa tactgtgtat gtcatcgaca
     9661 cagaaacggg cgcgatgctg tcaaaacggg aaccgggttt tggctgggaa atcgagtctg
     9721 acgggtttag caataccgtg gctgcggtac ggaaaaaaca agatgacgcg cgcaaagcgc
     9781 ggaaggcact tgccgacttg gtggcatcga cagcggaaac gttcccagaa ttgtcgcttg
     9841 aagataaggt caaagctgcc gcagatattt ttgttgttaa ggttcagatg tgtacagtgg
     9901 gaaataggcc gatgcgtccg cgttgttttg tggctgaata tttgaggaaa gtggtcggcg
     9961 gtcctaagag ggtgtatgat gttggcggaa cctttcccgg atttaatgtc gacttagaaa
    10021 gcggcgcgaa ggaggacatt atcgtcctgt gtttcgaagt gcggggaaat ggtacgaacg
    10081 cgtttgtttg tatcccgacg gttccggtga agttgtggga atgcgctacg tatatgatcg
    10141 tgcttgacgc atcgccgacg attgtcgagg atgccaaaga ggttttggcg aagaaggagt
    10201 gatgtgaaac agtttattga gaaggcggcg gtgctgaccg aggcactgcc gtacattcag
    10261 gatttcaaag gctcggtcgt gctggtgaag gtcggcggca gtgtcatgga ggtcgaggag
    10321 aacctcgatc gcctgctgac agacatcgcg ttcatgaacg cggtaggtat gaagacggtg
    10381 ctggtgcatg gcggcggcaa ggcgatctcg cgcggcatgg aggctgcggg cgtcacgccg
    10441 cagttcgtgc agggcctgcg cgtcaccacc gaggaggcca tgcgggtcgt cgagcgggtg
    10501 atcaaggatg aggtgaacgc caaggtggtg cggatgttgc agcggcactt tgcgaacgcg
    10561 cgcccgttgc acggcgattg gattttcggc gtcgtgaaga agaccgggac tgatccagtc
    10621 agcggagcgg agttggattg gggatttgta ggcgagccgg tgtcggtcga cacccggccg
    10681 gtcgcggaga tgctggatgc gggcattgtg ccggtggtga cgccgctggg tacggggccg
    10741 gacggcaagg tgtataacgt caacgcggat acggcggcgg cggctttggc caaagccatg
    10801 aaggtgcgca aactggcgtt tgtctcggat gtgccggggc tattgatgga catgaatgac
    10861 cccgcgtcgt tgctggctac gctgcggatc ggcagtgtgc gcaagctgaa ggagaccggc
    10921 gttgtgggcg gcggcatgtt gccgaagctg gatagttgtg tggaggcgat ccaggccggt
    10981 gtgggcaagg tccatctggt cgacggccgc atgccgcatt cgttgttgtt ggagattttc
    11041 acgaaaaaag gtgtaggaac ggagattatt gcagatgagt aagagtgacg acattgccgc
    11101 tttgtttaac cagtatgtga tgcataccta tgcgccgacc gtgacgttga ccagcggcag
    11161 cggctgcaag gtccgcgatc cggatggcat gatgtatctg gatttcacgg ccgggatcgc
    11221 ggtccataat gtgggccact gtcatcccaa ggtggtcaag gcggtgcagg atcaggtggc
    11281 cacgctgggg cactgctcca acctctttta caatgcgagc caggcactgt tggcgcagaa
    11341 gctttccaaa ctctctttga acgggaagtg ctttttctgt aattccggcg cggaagccaa
    11401 cgaagcgatg atcaagctgg cgcggctgtg gggcaacgcg aagggcaaat acgagatcat
    11461 ctgtatgcag aactcttttc acgggcgcac attagccact gtggcggcaa ccgggcaggc
    11521 taaaatccag aaggggtttg acccgttgcc gataggtttc actttcgccg agttcaacaa
    11581 tctcgactca gtgaaagccg ctgtcagcga acggactgtg gctgtgatgc ttgaggcggt
    11641 gcagggcgaa ggcggtgtta tgccggccac cgaagagttc atggtcggcg tccgcaagct
    11701 ttgcgacgaa aaaggcctgc tgatgttgtg cgacgaggtg cagtgcggca tggggcgtac
    11761 cggcaagtgg ttcgcctggc agcacttccc ggttaagccg gacgctttca cgttggccaa
    11821 atcgctggcg gacgggattc cgatgggtgc gctggtggca tcccccgcgt tgtcggacct
    11881 ttttcaaccg ggccaccacg cttcgacgtt cggcggcaat ccggtgtcga gcgccgcggc
    11941 gttggcggtg atggaagtta tcgaagaaga ggcgctggtc acacaggcgg aaaatgcggg
    12001 caccctgttc gtcgagggac tcgcgatgtt tgtcgaaaag tacgatcagg tgctggaggt
    12061 gcgcggcaaa gggttgatgg tcggcatggt ggtcgaggga tccgccaaag aggtcgtgga
    12121 cgcctgccgc gagatgggtc tgctctgttg tgtggctggc gagcatgtgg tgcgtttcct
    12181 gccgccgcta aacgtgaaag acagcgatct ggaggaagca ctggagatga tcggcgatgc
    12241 gttggatcaa cttttcggcg gggcggagga ggcctgacct cgtctcggct ataacctcaa
    12301 tgccgtatct tcttctggaa acaaccggac aaagtgaggt tgttttgact ttgagcagat
    12361 ggttgaaaaa actgatcggg atgagtctgc tggcgatggt tccgttgctc cgtgcagacg
    12421 tacaggtgat cgagacggtt tgtcctaatg gcgccaaggc ccgttatttc gaagagatgg
    12481 tgcccatgcc ggatggcgtg aagctgtaca cgtacggagc cgtgccgcct gacggcatga
    12541 agtgcccgat tgtcatccag cgtaacccgt atgtcaaaga cgagcgggtc aagcccgaga
    12601 cgttcgccgg tcagaggtgg accccggaca gctatgtctg gattacgcag cattgccggg
    12661 ggtgcggacg cagcgagggc gactggatcc cttatgccac ggaacgcgcg gatgggctgg
    12721 cgtttctgga gtggatacgg aagctgccct ttt
//
LOCUS       DAOSLE010000078        12009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5332_length_12009_cov_10.001840, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000078 DAOSLE010000000
VERSION     DAOSLE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12009
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_12009_cov_10.001840"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            785..1453
                     /locus_tag="PK576_06660"
     CDS             785..1453
                     /locus_tag="PK576_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61310.1"
                     /translation="MKAARANLVPGLILQAAAFALLASYTFLPPVRHALTEVGRWQAR
                     FGVPFSLASYLFFCGVVPYLFCLACPALRPAQRGRALAFALGFWGLMGLLIPQFYRLQ
                     SALYGNGTDVQTLVCKILTDQFVFSAFLTVPLVSLAHFWKDCGYRWEAVAPHMGRGWY
                     SRLVLPNLVMNWTVWIPSLCVIYSLPTALQPHVAGLIGGFWALMCLQIAAHTPKTTRQ
                     PDCG"
     gene            complement(1468..2739)
                     /locus_tag="PK576_06665"
     CDS             complement(1468..2739)
                     /locus_tag="PK576_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020585561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOE61311.1"
                     /translation="MATDLKAAYKTIMDDHFTPQMEITFLDGGKRQTLCYEKVSWVID
                     GEEKGLRYGENPGQEAALYKLVNGNLTLGEVTALTPGNVLASEPELLQSGKHPGKTNL
                     TDADNALNILRYLDATPCAVIVKHNNPCGVAKGPTLAEAYFRANKADRLAAFGGCIAL
                     NREVDIETARLVAENYAEVVVAPEFAPGVMDLFATKKNLRVMRIRNMARLTSFVARRV
                     VDFKSLIDGGLVAQWSFVPEARTPEQLLPAECTTKDGTVHRIRRLPTPQEYEDLIFGW
                     LVEAGVTSNSVLYVKDGATVGIGTGEQDRVGVAEIARDKAYRKLADWIAWEKYATPYN
                     ELVLKFGDLGKSRMGEIDEEVKARKGGLPGCSMVSDAFFPFRDGAEVGIREGITSIVQ
                     PGGALRDWDVIDCCNEAGVAMVFTGQRSFKH"
     gene            complement(2844..3677)
                     /locus_tag="PK576_06670"
     CDS             complement(2844..3677)
                     /locus_tag="PK576_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE61312.1"
                     /translation="MMLFFEDFLRFPFLRHAVLAVVLASVAAGLVGSLVVVRRATYLA
                     GAVSHSLLAGLGLARLLQRRFGIGFLTPMGGAVLTALAVVLLVTHLTARGRHRTDTVL
                     SAVWTGGMALGVSCVAATPGYQDDLMSYLFGSLLLVTGTDLFWMAVLDAVLVAVILLC
                     HNRFLAIAFNEELARLRGVNTALYELLFHIMTALTVVLLVRVVGIVLAIALLTLPAAA
                     AGRLTRSLDRMMLLAAGLCLLVSLIGLVVSYAPGWPPGATIVELAVLVYLGTSLTRAR
                     R"
     gene            complement(3715..4500)
                     /locus_tag="PK576_06675"
     CDS             complement(3715..4500)
                     /locus_tag="PK576_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOE61313.1"
                     /translation="MTAPVIDVTDVCFGYGSEEVLHNVSFAVLANTLVAVVGPNGGGK
                     TTLLKLLLGELVPRYGSIRVLGGAPAETRTRVGYVPQSLAFDPLVPVSAREVVLMGRV
                     GRARFGLYGGSDRRRADDALARVGLEDFGRRAFAELSGGERQRVLIAQALAGGSELLL
                     LDEPVANVDPEQTERLYALFQELTATVTVLMVSHNLSVVTDRATHVLCVNGTAELHAI
                     NEVASSTFAAAYGGRLTTIRHDASCHVLDASGVMHRPHHGEEG"
     gene            complement(4497..5393)
                     /locus_tag="PK576_06680"
     CDS             complement(4497..5393)
                     /locus_tag="PK576_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOE61314.1"
                     /translation="MSRWLSVSQHGRCLLAVLLSLRFLGGARGADRPLVLVSIPPQAW
                     LVKRLADGAVAVESLLADAANPHLFEPDARQARLLAEASIYFTLGLPFERAVLSRAAR
                     MPTRPAFHAMDAGIAKHGARHGHAAPGHACSGTEDGDPHIWLSPVLMCAMASNTVAAL
                     SERFPAHGDAFGAALGRTVAEICALDAEMRERLRPLATRTFAVYHPSWDYFAETYDLV
                     SLVLEREGKAPSARHLADLIRQARAAGVRVVFADPQYDPRPVHALASQLGARVVSLNP
                     LQETWPVLLRRAAAALADEETP"
     gene            complement(5390..5641)
                     /locus_tag="PK576_06685"
     CDS             complement(5390..5641)
                     /locus_tag="PK576_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOE61315.1"
                     /translation="MEARIGFVGILLEHRDSVPEVNRILSDHADRILARVGLPCRDRR
                     VSVITLVVEATTDQLGDLTGRLGQVAGVSVKSALSKERA"
     gene            complement(5674..6477)
                     /gene="proC"
                     /locus_tag="PK576_06690"
     CDS             complement(5674..6477)
                     /gene="proC"
                     /locus_tag="PK576_06690"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOE61316.1"
                     /translation="MKIGFLGAGAMAEAILASVLRNGLCKPGDVLACDKDEGRRVLMK
                     ERYGVRVTDDPAEVVRRYAVLLLAVKPQDLDALLSTLKPLLKARHLLISIAAGKTLAA
                     LKKAAGPHPRRIRVMPNLPLSVQEGMSVYCAAAPAKLADRRLVARLFGSAGAVLELPE
                     RQFDAVTALSGSGPAFVAFVAQALIAGAQALGLPPDAARLLTEQTLIGTGVYLQNTGR
                     DLDEFIRAVASPRGTTAAGLAVLRKSAVPATLARTLAAAAARSAELSRA"
     gene            complement(6542..7057)
                     /locus_tag="PK576_06695"
     CDS             complement(6542..7057)
                     /locus_tag="PK576_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61317.1"
                     /translation="MDLSEQQRQAVKEWLAEGASLSDVQKRLKSEFDLMLTYMDVRLL
                     ILDIGATVQDKPEPKPAEPAAESRVPPPEGEDPYDDVFDDEPGGGLPSGEDGAAGGKV
                     SLTLDRLVVPGAMFSGDVTFSDGVKARWMIDQIGRFGLDPEKPGYRPSDADLQAFQLQ
                     LRSELQRHGYA"
     gene            complement(7263..8387)
                     /locus_tag="PK576_06700"
     CDS             complement(7263..8387)
                     /locus_tag="PK576_06700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOE61318.1"
                     /translation="MAQAKHTREQVREVMESFPVWGDFRDAASYGSGHINDTYRVSVC
                     LAGTPVHYLLQRINHDIFRRPDQVMENIVRVTEHLYGRLTAAGESDVTRRTLTVVPAR
                     DGQPFTRDAAGNWWRLYLFIEQARTYDVIETVDQAREAARAFARFQNRLADLPAPRLH
                     ETIPAFHNTLVRLEALDHAVTEDVRGRRKDVADELAFIDARREACGRLLERHARGEIP
                     ERITHNDTKINNVMLDDATGEGVCVIDLDTVMPGLALYDFGDMVRSATAAAREDERDL
                     SQVGSRADMFEALARGYLAEAAFLNEAELEELVFSGPLITLTIGIRFLTDYLSGDVYF
                     KTHRPGQNLDRCRTQLHMVRCMEDQTAERERLVREITRAK"
     gene            complement(8408..9250)
                     /locus_tag="PK576_06705"
     CDS             complement(8408..9250)
                     /locus_tag="PK576_06705"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOE61319.1"
                     /translation="MKLTHPTADLYVPDGLAIGPALARTTHLGIGAHQDDLEFMTIHG
                     ILSCFGRSDRSFCGVTCTDGAGSSRSGVYGDYTDEQMKAVRRQEQRAAAMVGRYGAML
                     QLDFTSKEVKARDNSALVNDLAVILDAARPGTVYTHAPSDKHDTHVGVTRAVIAAIRS
                     LPADARPATVYGCEIWRDLDWLPDARKVVLDVSERGNIAAALSGVFDSQISGGKRYDD
                     AVLGRRRAHATFFESHAVDEATMLTFAMDLTPLVRDDTLSLADYTAGLIDEMKADVVR
                     RIGG"
     gene            complement(9340..10710)
                     /locus_tag="PK576_06710"
     CDS             complement(9340..10710)
                     /locus_tag="PK576_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOE61320.1"
                     /translation="MFTRGEPKKATLKRDFKPPITLLRELQRAARPGGKTLAIAWERE
                     NGYVSRYDLPLPPRAEDSPEVAQLAERCVKFLLWAAGGWRLMLSGPQKLCQSIKKQYS
                     ARGARAFDVAFMEKVYDRPMVVELRKPSEMPQAHERALVMDKRTNGCRIGFDLGASDF
                     KISAVKNGKAIYSDEFPWDPRNQTDPDYHYNKLNEGLKIAAAKLPRVDAIGGSSAGVI
                     VDNRIRVASLLRAIPEARYAEAQNMFVRLAEEWKVPIEVANDGDVTALAGLMSLGKKG
                     ILGVAMGSSEAVGFIDRNGCLTGRLSELAFAPVDLHPEAPADEWSGDIGVGAMYFSQQ
                     AVNHLAVKKGMRFPAKMALPQRLERVQERMAEGDVTALKIYQKIGLYLGYTVPWYREF
                     YGFDSMMILGRVTSGAGGVMILETARLILADQFPELAEEVEVFMPDEKARRVGQSVAA
                     ATLPLI"
     gene            10889..11854
                     /locus_tag="PK576_06715"
     CDS             10889..11854
                     /locus_tag="PK576_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE61321.1"
                     /translation="MTQPISNLRFEYMNTSERRVLNLEPLGLPNVPVLGYCHYRSPRP
                     DVPEHWHPGCVEIHLGVRNVVEFGFDGRTYRLGPGDVFVNLPGERHTVSAHPNGLILY
                     WLILRVGRSRIPFLGQPETEAEALRKSLLQLPHRHFRGTETLKRLFRSLHLLCDEPAT
                     HLRALRLRTTLLALLLEVVNAAEVHRDSPGETRLEALLREMRTAPEKAYRLDDLARRA
                     GMAPSHFIARFRSLTGLPPHHFLLHTRIEAARRLLRESDVPITELALRLGFCSSQHFA
                     HIFKRHTGITPRACRKGACGPLRAGDDNDGQSGEKRKEQANALMS"
BASE COUNT         2232 a         4038 c         3619 g         2120 t
ORIGIN      
        1 tggcggtcgg cccaagccgc ctgatcaccc cgacaacctt ctggcatcac gccggtcatc
       61 atgaatctcg acaaggcgaa gaccatagat ctccagtaaa agtcgatttc ccggaacccg
      121 gcgcctacgc gatcgaatac gtggtgaccg ccgcatctcc gcgctccaga taaatggcgt
      181 gtaatcggtt ggtgacaggg ggcatgcgag tttagtgcgt ggtttgtgtg tgcgcagtcg
      241 ggagcgcggg cgtcttccgc gctacgcgcg gatttaggat ttaggattta gcccgccgcg
      301 cggctctgcc gcgaaaaaac cttggaagta agtttaggac atgattaaca tgatgttgtt
      361 caatcctgaa aatcctgtaa tcctgtcaag taatcctggc aatttatagg caaggagttt
      421 ttcacttctc ccgtcagacc ttatgtcgac ccgcgacccg cgacgagcga cctgcggtgt
      481 cggaaacgcg ggcgtccctg tcgatcgtcg cgggtcgaat gtcgcgggtc gacgttcccg
      541 atcccgatac cgataccgat agcgatcccg atcccgattt ggatgaaacc ctgaaaccct
      601 gaaaccagaa accctgaaac cttgaaacca aagagcgttg aaggttcaac tgctcttttt
      661 aggtattgtc ttcgcacacc ccctgtctct gaccgattgc aaatggcgtc cgcacgggct
      721 ttgaccacac gggaacagat cgtttatact tatacggatg caaacaaatc cgtggatcat
      781 cggcttgaaa gccgcgcgcg ccaatctggt gccgggactg attttgcagg cggcggcgtt
      841 cgccttgctg gcctcatata cgtttctgcc gccggtccgc cacgcgctga cggaggtcgg
      901 ccgctggcag gcccggttcg gggtaccttt ttcactggcc agctatctct tcttctgcgg
      961 cgtggtgcct tacctgttct gtctggcctg tcccgcgctg cgtccggccc aacgtggccg
     1021 cgcgctcgct tttgcgctgg ggttctgggg cctgatgggg ctcctcatcc cgcaattcta
     1081 ccgtctgcag agcgcgttgt acgggaacgg caccgacgtg cagaccctgg tctgcaagat
     1141 tctcacggat cagttcgtct tttccgcgtt tctcacggtg ccgctcgttt cgctggcgca
     1201 tttctggaaa gactgcgggt accgttggga agcggtcgca ccgcacatgg gccggggatg
     1261 gtactccaga ctggtcctgc caaatctggt gatgaactgg acggtctgga tccccagcct
     1321 gtgcgtgatc tacagcctgc cgaccgcgct ccagccgcat gtcgccggac tgatcggcgg
     1381 tttctgggcg ctgatgtgcc ttcagatcgc ggcacacaca ccgaaaacga cacgccagcc
     1441 ggattgcggc tgaacgccgc tttcgcctca atgcttgaag ctgcgctgac cggtgaagac
     1501 catcgcgacg ccagcctcgt tacagcagtc gatcacatcc cagtcgcgca gagcgccgcc
     1561 cggctggaca atcgaagtga tcccctcccg gatgccgacc tccgcaccgt cacggaaggg
     1621 gaagaaggcg tccgagacca tgctgcatcc gggcagaccg cctttgcggg ccttgacctc
     1681 ttcgtcgatt tcccccatcc ggcttttgcc cagatcgccg aacttcagga cgagttcgtt
     1741 gtacggggtg gcatacttct cccaagcgat ccagtcggcc agtttgcggt aagccttgtc
     1801 gcgcgcgatc tcggccacgc ccacacggtc ttgctcgcca gtgccgatcc ccacggtcgc
     1861 gccgtccttg acatagagca cggaattgct ggtgacccct gcctcgacca accagccgaa
     1921 gatcagatcc tcgtactcct gcggagtggg cagacgccgg atgcggtgga ctgtgccgtc
     1981 cttggtcgtg cattcggcgg gcaggagttg ctccggcgtg cgcgcttccg gtacaaacga
     2041 ccactgcgcc accaggccgc cgtcgatcag cgatttgaaa tccaccacac gccgcgcaac
     2101 gaacgaggtg aggcgcgcca tgttgcggat gcgcatcaca cgcaggtttt tcttggtggc
     2161 aaacagatcc atcacaccgg gggcgaactc gggggccacg accacctcgg catagttttc
     2221 cgccacgagc cgggcggtct caatgtccac ctcgcggttg agtgcgatac agccgccgaa
     2281 cgcggcgagc cggtcagcct tgttggcgcg gaagtacgcc tcggcgaggg tcgggccttt
     2341 ggccaccccg caggggttgt tgtgcttgac gatcacggcg cagggcgtgg catccagata
     2401 gcgcaggatg ttgagcgcgt tgtcggcgtc cgtaaggttg gttttgcccg gatgtttccc
     2461 ggactggagc agctcgggct ccgaggcgag aacgttcccg ggcgtcaggg ccgtgacctc
     2521 gccgagcgtg aggttgccgt tcacaagctt gtacagcgcg gcctcctgtc ccggattctc
     2581 gccgtaccgc agccctttct cctctccgtc aataacccag gacacctttt cgtaacacag
     2641 ggtctgtcgt ttcccgccgt cgaggaaagt gatttccatc tgcggagtga agtggtcatc
     2701 catgatggtc ttgtaagcgg ctttcaaatc agtagccatg acgttccttt acgatatagg
     2761 ggtgagaagg ctcgcattcg taaatccgaa gtgtacggca tcccctttcg gttgtcaaaa
     2821 cccgtcggtc ctccgcagaa cgttcaccgc cgcgcacgcg tcagcgaagt gccgagatag
     2881 acaaggaccg cgagttccac aatcgttgcg cccggcggcc agccgggcgc gtagctcacc
     2941 acaagcccga tcaggctaac caacaggcag agtcccgcgg cgagcagcat catgcggtcc
     3001 agactccgcg tcagacgccc tgcggccgcg gcagggagcg taagcagggc gatcgccaga
     3061 acgatgccga ccacccgcac cagcaacacc acggtcagcg cggtcatgat gtgaaaaagc
     3121 agttcgtaga gcgcggtgtt aaccccgcgc agacgcgcca gctcctcgtt gaacgcgatc
     3181 gccagaaaac gattgtggca gaggaggatc accgccacca gcactgcgtc cagcaccgcc
     3241 atccagaaga ggtccgtgcc ggtcaccagc agcaacgagc cgaacagata actcatcagg
     3301 tcatcctgat atccgggtgt ggccgccacg caagaaacac ccagcgccat gccgccggtc
     3361 cacaccgcgc tgaggacggt gtcggtgcgg tgtcggccgc gcgccgtcag atgcgtcacc
     3421 agcagaacca cggccagcgc tgtcagcacc gcgccaccca tgggggtcag gaacccgatc
     3481 ccgaaacggc gctgcagcag gcgcgccaaa ccgagccccg ccagcagact gtgcgaaacg
     3541 gccccggcca gatacgtggc gcggcgcacc accaccaggc tgccgaccag cccggccgcg
     3601 accgacgcca gaaccacggc cagcacggcg tgccggagaa aggggaaacg caggaaatcc
     3661 tcgaaaaaaa gcatcatgcc cccgttttgt tcttttcgcc cattccctcc cccttcatcc
     3721 ttcttcccca tggtggggcc ggtgcatgac tccggaagcg tcaagcacat gacaggaggc
     3781 gtcgtggcgg atcgtcgtca ggcgtccgcc gtaggcagcg gcaaaggtgg aggaggcgac
     3841 ctcgttgatg gcatgcagtt cggcggtgcc gttgacgcaa agcacatgcg tcgcgcgatc
     3901 cgtgaccaca ctcagattgt gcgagaccat cagcaccgtc accgtggcgg tcagctcctg
     3961 aaagagcgca tagaggcgtt cggtctgttc gggatcgacg ttcgcgaccg gctcatcaag
     4021 cagcagcaac tccgagccgc ccgccagggc ctgcgcgatc aacacccgct ggcgttcgcc
     4081 tcccgagagt tcggcaaagg cccggcggcc gaagtcctcc aaccccacgc gcgccaacgc
     4141 atcgtccgcc cgcctgcggt ccgacccgcc gtacaagccg aaccgggcgc gtcccacgcg
     4201 gcccataagc acgacttcgc gcgcgctgac cggcaccaat ggatcaaagg ctaaagactg
     4261 cggcacgtag ccgacgcgcg tgcgcgtttc agcgggtgcg ccgcccaaca cgcggatcga
     4321 gccataacgc ggaacgagtt cgcccagcag caatttcagc agcgtggttt tcccgccgcc
     4381 gttggggccg accaccgcga ccagggtgtt cgccaagacc gcgaaggaga cattgtgcag
     4441 cacctcctcg ctgccatagc cgaaacaaac atccgtcacg tcgatcaccg gcgcggtcat
     4501 ggggtctcct catcggccag agccgcggcc gcacgacgca acagcacggg ccacgtctcc
     4561 tgcagcggat tcagcgaaac cacgcgcgcg cccagttggc tggccagcgc gtgcacgggc
     4621 cgcggatcgt actgcggatc ggcgaagacg acccggaccc cggcggcgcg cgcctgccgg
     4681 atcagatccg caaggtgccg ggccgacgga gccttgcctt cccgctccag caccagcgaa
     4741 acgaggtcgt aggtttcggc gaaatagtcc caggaggggt gatacactgc gaatgtgcgc
     4801 gtcgcgagcg gacgcaggcg ctcgcgcatc tccgcgtcca atgcacaaat ctccgccacg
     4861 gtgcgcccga gggccgctcc gaacgcgtca ccgtgcgccg gaaagcgttc gctgagggcg
     4921 gcaaccgtat tggaggccat cgcgcacatc aggaccggac tgagccagat gtgcggatcc
     4981 ccgtcctccg tgccggagca ggcatgacct ggcgcggcgt ggccgtgacg cgcgccgtgc
     5041 ttcgcgattc ccgcgtccat ggcgtggaac gcgggccgcg tgggcatgcg ggccgcgcgg
     5101 gagagcaccg cccgctcgaa cggcaggcca agcgtgaaat aaatcgaagc ctccgcaagg
     5161 agacgcgcct gccgcgcatc gggttcgaag agatgcggat tcgccgcatc ggccagcaac
     5221 gactcgaccg cgacagcccc gtcggccagc cgtttcacca gccacgcctg cggcggaatg
     5281 gagaccagca ccaatggccg gtccgcgccg cgtgcgccgc ccagaaaccg tagcgacagc
     5341 aacaccgcga ggaggcaccg gccatgttgg gaaacggaga gccaacggct catgcacgct
     5401 ccttggagag ggcggacttg accgaaaccc cggcgacttg ccccagtcgt cccgtgaggt
     5461 cgcccaactg gtccgtggtg gcctcgacca ccagcgtgat cacggaaacc cgcctgtcgc
     5521 ggcaaggtaa gcccacacgt gcgagaatcc ggtccgcatg atccgacaag atgcggttga
     5581 cttccggcac gctgtcgcgg tgttcgagca ggatgccgac aaaaccgatg cgcgcttcca
     5641 tttcctcccc tttcccttcc gatccgtgcc gtctcaggcc cggctgagtt ccgcgctacg
     5701 cgccgcagcc gcggccagag tccgggccaa ggtggcgggc accgcggatt ttcgcagcac
     5761 cgccagacct gcggcggtgg tgccgcgggg cgaggcgact gcgcggatga actcatcgag
     5821 gtcgcggccg gtattctgca gatagactcc ggtgccgatc agcgtctgct cggtcagcag
     5881 gcgcgcggcg tcagggggca gacccaaagc ctgtgcgccc gcgatcaggg cctgagccac
     5941 aaaggccacg aacgccggtc ccgaaccgct cagggctgtg acggcgtcga actgccgctc
     6001 cggaagttca agcaccgcgc ccgcgctgcc gaagagccgg gccacgaggc gtcggtccgc
     6061 tagtttggcg ggggccgcgg cacaatagac gctcatgccc tcctgcacgc tcagcggcag
     6121 gttaggcatc acgcggatcc gccgcgggtg gggtccggcc gcttttttca gtgccgccag
     6181 agtcttgccc gccgcgatcg aaatgagcag gtgccgcgcc ttcagcaacg gtttgagtgt
     6241 gctcaacagg gcatcgagat cctgcggctt caccgccagc aacaacaccg cgtaacgccg
     6301 cacgacctca gccgggtcat cggtgacacg cacaccgtaa cgctctttca tcaaaacgcg
     6361 ccggccctca tctttgtcgc acgccaacac gtcgccgggt ttgcacagac cgttgcgcaa
     6421 caccgaggcg agaatcgcct ccgccatagc gcccgcacca agaaaaccga ttttcatccc
     6481 tgtctcccaa aaatcaagct caacaacgca tgcttctttg ccgtgatcgg ttcgtcaggc
     6541 actacgcgta accgtgacgc tgcagttccg atcgcaactg aagctggaac gcttgcagat
     6601 ccgcatccga aggccggtag cccggttttt ccggatccag accgaaacgg cctatctgat
     6661 cgatcatcca gcgggccttc acgccgtccg aaaaggtcac atcgccgctg aacatggcac
     6721 cgggaaccac cagacggtcg agcgtcaggc tcaccttgcc gcccgccgcg ccgtcctcgc
     6781 cggacggaag cccgccgccg ggttcgtcgt cgaatacgtc gtcgtacgga tcctcgcctt
     6841 cgggcggcgg cacacgtgac tccgcagccg gttcggccgg tttgggttcg ggtttgtctt
     6901 gaaccgtcgc gccgatgtca aggatgagaa gccggacgtc catataggtc agcatgaggt
     6961 cgaactcgct cttcaaccgt ttctgcacat cggagagcga ggccccttca gccagccact
     7021 ctttgacggc ctgacgctgt tgctcggata aatccatgat tgccatctcc ttttccagtg
     7081 attgtcccaa gcctgtgtgc aaacgtcaag gttgcatcgg aaaagacgca tttgatttcg
     7141 gggcgcatgc ggatctctcg gatccgactg atccgtcgga tcggaggtgt cccggtcgca
     7201 ggtcggcgta cacgcgaacg agagggggaa acgatcccga tagcgacaaa cccgaacggc
     7261 gatcacttcg cgcgcgtgat ctcccgtacc agcctctcgc gctccgccgt ctgatcctcc
     7321 atgcagcgca ccatgtggag ttgtgtgcgg caccggtcca gattctgtcc ggggcggtgg
     7381 gttttgaaat acacgtcacc gctgaggtag tcggtcagga accggatgcc gatcgtcaag
     7441 gtgatcaacg ggccggagaa gacaagctcc tcaagttcgg cctcgttgag gaacgcggcc
     7501 tccgccagat agccgcgcgc cagggcctcg aacatgtcgg cacggctgcc gacctgcgag
     7561 agatcacgct catcctcgcg cgccgcagca gtggcagagc gtaccatatc gccgaaatca
     7621 tagagcgcga gacccggcat cacggtatcg aggtcgatca cgcagacccc ctcgcccgtg
     7681 gcgtcatcca gcatgacgtt gttgatcttg gtgtcgttgt gcgtgatccg ctccggaatc
     7741 tcgccacggg cgtggcgttc cagcagccga ccgcacgcct cgcggcgcgc gtcaatgaag
     7801 gcgagttcgt cagccacgtc tttcctgcgg ccgcgcacat cctccgtcac ggcgtgatcc
     7861 agcgcttcga gccgcacgag ggtattgtgg aacgcgggga tggtctcgtg cagacgcggc
     7921 gcgggcaggt cggcaagccg gttttggaaa cgtgcgaacg cgcgcgccgc ctcacgcgcc
     7981 tgatccacag tctcgatcac atcataggtg cgcgcctgtt cgatgaagag gtagagccgc
     8041 caccagttgc ccgccgcgtc gcgcgtgaag ggctggccat cgcgtgccgg gaccacggtg
     8101 agggtccggc gcgtcacgtc gctctccccg gcggcggtca gccgaccgta caggtgctcg
     8161 gtcacgcgca cgatattctc catcacctga tcgggccgtc ggaagatatc gtggttgatt
     8221 cgttgcagca ggtaatgcac cggcgtgccg gccaggcaga cagaaacccg ataggtgtcg
     8281 ttgatgtggc ccgagccgta ggacgcggca tcgcggaaat cgccccacac cggaaaggac
     8341 tccatcacct cccgtacctg ttcacgcgta tgtttcgcct gtgccatacc ccctccccgg
     8401 ccgacgatca gccgccgatg cgccgcacca catccgcctt catctcatcg atcagtccgg
     8461 ccgtgtaatc cgcgagcgaa agggtgtcgt cccggaccaa cggtgtgagg tccatcgcga
     8521 aggtcagcat ggtagcctcg tccaccgcgt gcgactcgaa aaaggtggcg tgtgcccggc
     8581 gccggccaag cacggcgtcg tcataccgtt tgccgccgga gatttgcgaa tcgaacacac
     8641 cggagagggc ggcagcgatg ttgccccgct cactcacgtc cagcaccacc ttccgggcgt
     8701 ccggcaacca gtcgaggtca cgccagattt cgcacccgta cacggtggcg ggccgcgcgt
     8761 cagcgggcag gctgcggatc gccgcgatca ccgcgcgcgt cacgcccaca tgggtatcgt
     8821 gcttgtcgga gggcgcatgc gtgtacaccg tcccgggccg ggccgcatcg agaatcacgg
     8881 ccagatcgtt gaccagcgcg gaattgtcgc gcgccttcac ctctttgctg gtgaagtcca
     8941 gttgcagcat cgcgccgtac cggcccacca tcgccgcggc acgctgttcc tggcggcgca
     9001 cggccttcat ctgctcatcc gtgtagtcgc cgtaaacacc ggagcgcgag ctgcccgcgc
     9061 cgtccgtgca ggtcacgccg cagaaggagc ggtcgctccg gccgaagcaa ctcaggatgc
     9121 cgtggatggt catgaactcg aggtcgtcct gatgcgcgcc gatcccgaga tgcgtggtgc
     9181 gggccagagc cggaccgatc gccagaccgt ccggcacata cagatccgct gttggatgag
     9241 tcagtttcat attgcgttac tcctattttc ggttgccgtg atcacggggg gggggacacg
     9301 gagacgagag taaaaaacga tccgcgccgc acgctcccgc taaatcagcg gaagggtcgc
     9361 ggccgccaca gactggccca cgcgccgcgc cttctcgtcg ggcatgaaca cctcgacctc
     9421 ctccgcaagt tcgggaaact ggtcggccag aatcagccgt gccgtttcca gaatcatgac
     9481 cccgcccgcg ccggacgtca cgcgcccaag gatcatcatg ctgtcaaagc cgtaaaactc
     9541 acggtaccac ggcacggtgt agccaaggta gaggccgatc ttctgataga tcttcagagc
     9601 cgtcacgtcc ccttccgcca tacgctcctg cacgcgctcc aagcgttggg gcagcgccat
     9661 cttggccggg aaacgcatgc ccttcttcac cgccagatgg ttcaccgcct gctgcgagaa
     9721 gtacatcgcg ccgacaccga tatcccccga ccactcgtcg gccggagcct ccgggtgcag
     9781 atcaaccggc gcgaaagcga gttccgagag acgcccggtc aggcacccgt tgcggtcgat
     9841 aaaaccgacg gcctcgcttg atcccatggc cacgcccaga atccctttct tgccgagcga
     9901 catcagaccg gccagcgccg tcacgtcgcc atcgttggcg acttcgatcg gcaccttcca
     9961 ctcctccgcc agacggacga acatgttctg cgcctcagca taacgcgctt ccggaatcgc
    10021 acgcaggagc gaagccaccc gaatccgatt gtccacgatc acgccggcgc tgctaccgcc
    10081 gatcgcgtcc acccgcggca gcttggctgc ggcaatcttc aatccctcgt tcaacttgtt
    10141 atagtggtaa tccggatcgg tctggttgcg cggatcccac gggaactcat cgctgtagat
    10201 cgccttgcca ttcttgaccg cgctgatctt gaaatcgctc gcccccagat cgaagccgat
    10261 tcggcagccg ttggtgcgct tgtccatcac gagcgcacgc tcatgcgcct gcggcatctc
    10321 gctcggcttg cgcagttcca ccaccatcgg acggtcatag accttttcca tgaaggccac
    10381 atcaaacgcc cgcgcgccgc gcgccgagta ctgctttttg atcgactggc acagcttctg
    10441 cggaccggag agcatcagac gccagccgcc ggcggcccag agcagaaact tcacgcaccg
    10501 ttccgcgagc tgcgccacct ccggcgaatc ctcggcgcgc ggcggcaacg gcagatcgta
    10561 acgcgaaacg tagccgttct cgcgctccca agcgatggcc agcgtctttc cgcccggccg
    10621 cgccgcgcgc tgcaactctc gcagtagtgt gatgggcggt ttgaaatcgc gtttcaacgt
    10681 cgctttcttc ggttcgccgc gggtgaacat cgtcagcctc cttctgggtt acgggggccg
    10741 cgcgttgaaa agtgcgtcag cctttgcgga cacttccgcc tgccgccgca cgcatgcgcg
    10801 aacccgccca cacgttcttt ccggccgcag aataggcgat gttctcttcc gcttgcgttc
    10861 atctttttcg tttcataaga ttacgcacat gacgcaaccc atctccaatc tgcgtttcga
    10921 gtacatgaac acctccgagc ggagggtgtt gaatctggag cccctgggtc tgccgaacgt
    10981 gccggtgctg gggtactgcc actaccgcag cccgcgccct gacgtgccgg agcactggca
    11041 tcccggttgc gtcgagatcc atctcggagt gcgcaacgtg gtggagttcg gctttgacgg
    11101 ccgtacctac cgcctcggtc ccggcgacgt gttcgtgaac ctgcccggcg agcgccacac
    11161 ggtcagcgcg catcccaacg gcctgatcct gtactggctg atcctgcgcg tgggacgatc
    11221 ccgcattccg tttctgggcc agccggagac tgaagccgaa gcgttgcgga aatcgctgct
    11281 ccaactcccc catcgtcact tccgcggcac cgagaccctg aagcggcttt tccgttcgct
    11341 acacctgctc tgcgatgaac cggccacgca cttgcgcgcc ctgcggctgc gcaccaccct
    11401 gctggcgctg ctgctggagg tggtgaatgc ggcagaggtc caccgcgact ccccgggcga
    11461 gacgcggctg gaggccctgc tgcgcgaaat gcggaccgcg cccgagaagg cataccggct
    11521 ggatgacctc gcccgccgcg cgggcatggc cccctcgcac ttcatcgcgc gcttccgcag
    11581 cctgaccggc ctgccgccac accacttcct gcttcacacc cgcatcgagg cggcccgccg
    11641 cctgctgcgc gagagcgacg tgccgatcac cgaactcgcc ctgcgcctcg ggttctgctc
    11701 gtcccagcac ttcgcccaca tcttcaagcg ccacaccggc atcacgcccc gcgcgtgccg
    11761 taaaggtgcc tgcggcccgc ttcgcgcggg tgacgacaac gacggccaaa gcggagaaaa
    11821 acggaaggag caagcgaatg cactaatgag ctaatgaact aatgcgggcg gagcccgcaa
    11881 tcccaaaggc tgtgggactg tggggctacg gttaaacaat cgatcccgat agcgatcccg
    11941 atagcgatcc cgatcccgac cccgatttgg atgaaaccat gaaatacatg aaacttcatg
    12001 aaaatcagg
//
LOCUS       DAOSLE010000079        11912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5377_length_11912_cov_8.240955, whole genome shotgun sequence.
ACCESSION   DAOSLE010000079 DAOSLE010000000
VERSION     DAOSLE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5377_length_11912_cov_8.240955"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1645
                     /gene="guaB"
                     /locus_tag="PK576_06720"
     CDS             152..1645
                     /gene="guaB"
                     /locus_tag="PK576_06720"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOE61322.1"
                     /translation="MHVNTQVSAFMDQFPCEGLTFDDVTLVTRYADFLPDETNLASPL
                     TSRITLNIPFVSAAMDTVTEFGMATAMAMLGGIGVIHKNLPPELQAQHVRRVKQYLNG
                     LIMRPVVFHTQDTLAHIQAYRNEHRLSFSGFPILDDQDRLAGIITSSDIRFAKKSASC
                     VTEVMTTRVITAAPGTSLEEAYAIMMENKIGKLPLVDGGGRLVGLYAFTDVQELVENA
                     QPLYTRDSRYRLRVAAAVSAGDHERMGLLAEEDVDVVVVDSAHGHSKGILDMVTWITK
                     HYPDIDVIGGNVATGEGALALLKAGAHGVKVGIGPGSICTTRVVCGVGIPQITAVYHA
                     ARALGGEIPVIADGGVRHSGDVPKALVAGADTVMLGSILAGTEESPGEKIIHEGRHYV
                     VYRGMGSLAAMKEGMGSRQRYGQDNVQTDDLVPQGIEGIVPLVGSVKKTMTQFCGGLR
                     SSLGYCGSRNLAELKANGCFFRVSNAGVREAHPHDVKVIKEAPNYRS"
     gene            complement(1681..1926)
                     /locus_tag="PK576_06725"
     CDS             complement(1681..1926)
                     /locus_tag="PK576_06725"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE61323.1"
                     /translation="MKSETGTPATAGKTRNLLDLQDGETGRIRSNRDRKSAEMGLFAG
                     VRVTMFRNRKSEHSVVVGAGDARFLVSRPIAEHIELA"
     gene            complement(1916..4168)
                     /gene="feoB"
                     /locus_tag="PK576_06730"
     CDS             complement(1916..4168)
                     /gene="feoB"
                     /locus_tag="PK576_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOE61324.1"
                     /translation="MRDDLTIALAGNPNTGKTTLFNLLTGAHERTGNWTGVTVDTAHA
                     AFTLNGKTARVTDLPGIYSFSPHTQDERVARDFLIQQKPAVIVNLVDAVNLERSLYLT
                     AQLLDMRLPLVVALNQTDVAERYGIHVDADHLASHLGCPVIPMVASKGVGLEALKQAI
                     CHVAETRSLPTTRVQYGDAEPSVRRLQVCLIVAADDAGVDSRWLAIKLLERDPFAREL
                     TAGLFDELAEEECARIARHTGQKPEEAVMDARLGFIRGLARDVVQRKRVSRRRVSDVA
                     DRLLLSRPLGIPFFLGVMYVVFWLTVRLTQPLVDFTDLALNALLVNAPRIWLARLGMP
                     EILGSLLCDGVGAGLTTVATFMPPIFMIFLCLSLLEESGYMARAAFIMDRFLNRIGLP
                     GKAFIPLLVGFGCTVPALLATRTLEQRRDRLLTMLIAPFMSCGARMPVYAIFAMAFFP
                     NQGNIVIFSLYLTGVVLAILSGLMLHRTLFRGGAASAFVMELPPYHLPALRGCLEHVW
                     ANLQSFLVRAGQVILTIAILLSLANALFEKAYRNPETAFAHRSAAGRVLTVPLRPMGV
                     TGDNWPAAAALLTGLMAKEAIVGTLEVLYFQTEKTAPDSRKPFDLRAELRAAGSALFA
                     GFSGSAATADPDRSADPAARQGLLSTLRRNFASPTAAYAYLLFVLIYSPCVAALIVLA
                     REAGWRWMGFAVTYQSLLAWLTATVFYQCASFPAAPLRSVGWLAFVTALTAAGIALLA
                     AYGKRIAHEV"
     gene            4348..5121
                     /locus_tag="PK576_06735"
     CDS             4348..5121
                     /locus_tag="PK576_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase cyclase
                     subunit"
                     /protein_id="HOE61325.1"
                     /translation="MSQPVRIMPCLDMRDGRVVKGVRFVDIRDAGDPVACARAYCEKG
                     ADELAMLDITATVEGRATLLQVVRNVAEAVTVPFTVGGGISDVKTARAVMEAGANKVS
                     VSSAAFRKPALIPELVAELGAERVTVAIDVDRNAALPSGYEVYIDGGRTATGHDAVEW
                     AKRVDGLGVRVILPTSKAGDGARTGYDLPVIRAIRQAVSAEVVASGGAGTLEHFHEAV
                     EAGATILLAASIFHFGIIGIPELKAYLRERGVKVCGGEA"
     gene            5153..5734
                     /locus_tag="PK576_06740"
     CDS             5153..5734
                     /locus_tag="PK576_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE61326.1"
                     /translation="MKVVAFNGSPRQDGNTALLLRKVLAPVARSGIDTELVQVGGHAV
                     HGCTACMRCMTARNRQCAITTDRVNTWIAKMIEADGIVMGSPSYFSGMTPELKALLDR
                     AGLVAVANGRMFARKIGAAVAAHRRGGAVNVVDAINHLFLMSRMIVPGSTYWNFGVGL
                     EREDVADDAEAMENMHDLGETLVWLLKRLHADT"
     gene            complement(5777..6448)
                     /locus_tag="PK576_06745"
     CDS             complement(5777..6448)
                     /locus_tag="PK576_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61327.1"
                     /translation="MRKTVAFLALGLALLAAVMAFQRAIREEGRRIRSVFHQALAEPD
                     GLRERQADLLRKAETLNVQLATLNRRLSHLESRAPKETRAAPTGSAAPHSPAALRTDE
                     FRAAIGHLNALPDLLTELTAYLDQAFEHLEETVTVTAVPEDFAERLAAMEKRLADIDS
                     YFTPLYAFLGLVYDPANEAVLAAYPTLDKRISALDEKLVQLQRDLAALRESLEPRTKE
                     PRPVR"
     gene            complement(6514..7968)
                     /locus_tag="PK576_06750"
     CDS             complement(6514..7968)
                     /locus_tag="PK576_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOE61328.1"
                     /translation="MKKSYWVTLLRFLVGWHFLYEGLWKLLSGSWSAEPYLSSSEWIA
                     AKLFHTIAETPALLKVVDLLNIWGLTLIGLALILGVAIRFASISGIVLLALYYLAHPP
                     FTAPAGGESHLLWIDRNVIEAFALAVICVIPSYGLGALFQQYRNRRESPIAFDRREFL
                     KNLSSLPVLGAFAGAFIEKHGPVWEKEHLKSIADAMTGPTLRTFESKDISKLKKPIEA
                     FGQIGSTRLSRMFMGCNLIGGWAHARDLIYVSQLVKAYHEDWRVFRTLHMAEAAGMNT
                     IILNPGLFRVMGDYLKKEGGKMQVLSDCAAGGNPIKGAEISIKFGAQMLYIQGEMTEK
                     YIIREKNYKIMEDALAFMRKEGYQAGIGAHSLEAVKECVDRGLIPDYWVKTIHPINYW
                     SANINGGTRHDNIWCTNPKETIAYMKERPEPWIGFKTLAAGAIRPKDGFPFALKAGAD
                     FLCVGMYDFQLVDNVNLFNEIFPSCQERERPWRA"
     gene            complement(7999..8865)
                     /locus_tag="PK576_06755"
     CDS             complement(7999..8865)
                     /locus_tag="PK576_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE61329.1"
                     /translation="MRIRTLYLCLLAGFMTCHSAVAEPLRLATFNIRLPADKGVNAWS
                     NRAPRVIALVKRHGFDLVGLQEATAMQFDEMLAALPGWAMVGVCRDDGKRKGEATCIL
                     YRADRFTVQKSGTFWLSETPEVPGSKSWKTACTRVCTWALMTDTKNNKPFAFFNTHLD
                     HISVEARANGMKLILERMQTLVPQGWPIFLTGDMNCNETSEPVQLAGKVLRDAVRHTQ
                     TPHQGPWRTFTGYRYRPDAEEPKAPAKDAPRIDYIFVSEKVTVRDVTTHNDAENGNYP
                     SDHFPVSATVVF"
     gene            complement(8949..11846)
                     /locus_tag="PK576_06760"
     CDS             complement(8949..11846)
                     /locus_tag="PK576_06760"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOE61330.1"
                     /translation="MNVQDKRRGAISQPARLTATTLFLLTGFTGVAFPCFSHGAARVK
                     EITLEMPTYGFSAPNPIPEPETRVDRQIWPYFKFEGYTAVATTQSWTCVRLENDAVEV
                     DVFPQIGGKVWSARDKRTGRDFLYRNRVVKFRNISTRGPWTSGGIEFNFGMLGHAPTA
                     STPVDYACRTNSDGSASCFVGVLELITRTRWCVEIRLGASDRFFTTQTTWHNASGLPQ
                     PCYQWMNAAYPCGGRYVFPGTARLEHDGTASPWPRDASGLDLSVYTNNAFGLSKSYHV
                     VNGDTGFFGVWWDAHQFGSMHEAARFDRLGRKIWIWSLSRSGAIWEDLLTDEDGQYVE
                     LQSGRYFNQPSHGSHRTPFKYPSFPAGSTITFSERWSPVTEARILEERAAESGTVSRP
                     LATPADFDWGTVYGQTLQAEQDVNLKDYTAAEKTLRAVLAKEPHYLPALVCMADLLLH
                     CGRPAEALAFSERALAVDAYHDKANHLSGIVFAERGDHANALERFGAAALSPLFRSAA
                     ITRIAALECAAGRPDRMLEAAERALEANPLEFSALHLRLVALRLLGREDEHRAACAAV
                     LDRFPLFHPARCEQDRAAFAAGIRCELPHEEILETAHLYAAAGLFGEAAEIAALASGR
                     FLAAVARAYWLARTGDETAARNALADAERLDTAFAFPFRPETLPILEWAHAHSPSWKP
                     RYALALLLRHFGNDDRARALLNETDADDVPFLLFRARLRDGPSALADIRKAQTFGENR
                     YAVKAEWRAHAEANDWRQALGAAQRLLAAHPEDNDAGILLAQSHLRLGDPARCLETLD
                     TVRFLPSEFTSITRRVYREACLAQAVAAFRKSDSAVTRDAVRRAGIWDEKLGVGKPYA
                     SEIDMRLELWLFAQLEQGVTRADLLDRVAAGFAPSRPARTANALDLLTALACRERGQS
                     AKADAVAAAFGTDAVGVWCRAVFEGDRATASERLPAASSDENVRLLAALLL"
BASE COUNT         2261 a         3503 c         3957 g         2191 t
ORIGIN      
        1 ccatcaaagt tatcctcatt tacattcccg tgcgaagcgc gggcggcggg ctaacttctg
       61 tccacttctc ccttcgtgag tcttgcttta acgcagcatt tgagttatat aatagcggga
      121 aagcgaaacg aagttagttc ggaggttccc aatgcatgtc aatacccaag tctccgcttt
      181 catggatcag ttcccgtgtg aagggctgac ctttgacgat gtcacgctcg tgacccggta
      241 cgccgatttt ctcccggacg agaccaacct cgcctcgccc ctcaccagtc gcatcacgct
      301 gaacatcccg ttcgtgagtg cggccatgga cacggtcacg gagttcggca tggccacggc
      361 gatggctatg ctcggcggca tcggtgtgat ccacaaaaat cttccgcccg aacttcaggc
      421 gcagcatgtc cggcgagtga agcagtatct caacggtctc atcatgcggc cggtcgtgtt
      481 ccacactcag gatacccttg cgcacatcca ggcctatcgg aatgagcacc gcctgtcttt
      541 cagcggtttc ccgattctgg acgatcaaga ccgtctcgcg ggaatcatca cttccagcga
      601 tatccggttc gccaaaaaaa gcgcgtcgtg cgtgaccgag gtgatgacga cacgggtcat
      661 caccgccgct cccggaactt cgctggaaga ggcgtatgcc atcatgatgg agaacaagat
      721 cggcaaactg ccgctggtgg atggcggcgg ccgcctggtc gggctctatg cattcaccga
      781 tgtgcaggag ttggttgaga acgcccagcc tctttatacc cgcgacagcc gttaccgtct
      841 gcgtgtggcg gcggccgtca gcgcggggga tcatgagcgc atgggcttgc tcgcggagga
      901 ggatgtcgat gtcgtggtgg tggacagcgc ccacggccat tcgaagggga ttctcgatat
      961 ggtgacgtgg atcacgaagc actatccgga catcgacgtg atcggcggga acgtggccac
     1021 gggcgaaggc gccctcgcgt tgttgaaggc cggtgcccac ggcgtgaagg tcgggatcgg
     1081 acccggttcc atttgcacca cccgcgtggt gtgcggcgtg ggcatcccgc agatcaccgc
     1141 cgtgtaccac gcggcacggg ctctcggtgg cgaaattccg gtcatcgccg acggcggtgt
     1201 ccgtcattcg ggggacgtcc ccaaggcatt ggtcgcgggg gcggacaccg tgatgctcgg
     1261 ttcgatcctc gcgggcacgg aggagagccc cggagagaaa atcatccacg agggacgcca
     1321 ttatgtggtg taccgcggca tggggagcct ggccgcgatg aaagagggca tgggcagtcg
     1381 ccagcgttac gggcaggaca atgtccagac cgatgatctg gtgccgcagg gcatcgaggg
     1441 tattgtgccg ctggtcggct cggtcaagaa aaccatgacc cagttctgcg gtggcttgcg
     1501 ctcctccctc gggtactgcg gttcgcggaa tttggcggaa ctcaaggcca acggctgttt
     1561 cttccgcgtc tccaatgccg gtgtgcgcga ggcccatccc cacgacgtca aggtcatcaa
     1621 agaagcgccc aactaccgct cctgatccgg atctcggatc gtacggcaat gcgggaaaaa
     1681 tcacgcgagt tcgatgtgtt cggcgatcgg gcgcgagacg aggaagcggg cgtcgcccgc
     1741 gccgaccacc acggaatgtt cggatttgcg gttgcggaac atggtgacgc gcacgcccgc
     1801 aaaaagtccc atctccgcgg atttacggtc gcgattactg cggatgcgcc cggtttcccc
     1861 gtcctggagg tccagcaggt tccgcgtttt tcccgctgtg gcgggggttc cggtttcaga
     1921 cttcatgtgc gatccgtttc ccataagcgg ccagtagggc gattccggct gcggtgaggg
     1981 ccgtcacaaa cgccaaccag cctacggacc gcaagggtgc ggcggggaag gaggcgcatt
     2041 gataaaaaac ggtggcggtc aaccaagcca gtaaggattg gtaggtgacg gcaaacccca
     2101 tccaacgcca gcccgcctcg cgtgcgagca cgatcagggc cgcgacacag ggcgaataga
     2161 tcaggacaaa gagcaagtag gcgtatgccg cggtcgggga ggcgaagttc cggcgtaacg
     2221 tggaaaggag tccctgccgg gcggccgggt cggcggaacg gtccgggtct gcggtggccg
     2281 cagacccgga gaatccggcg aacagcgcgc ttcccgcagc gcggagttcc gcgcgcagat
     2341 caaagggctt ccgcgaatcc ggagccgtct tttccgtttg aaaataaagc acctcaagcg
     2401 tccccacaat cgcctctttg gccatcaggc ctgtgagcag ggccgctgcc gcgggccagt
     2461 tgtcccccgt cacacccata ggacggagcg ggacggttag aacccggccc gccgcactcc
     2521 ggtgcgcaaa agccgtttcc gggttacggt acgctttttc aaaaagggca ttggccagac
     2581 tgagcagaat cgcgatcgtc aaaatgacct gacccgcccg gaccagaaag ctttggagat
     2641 tggcccagac atgctccaga catccgcgca aggccggcag gtggtagggc ggcagctcca
     2701 tgacgaacgc tgaggcggct ccgccgcgga agagggtgcg gtgcagcatc agaccggaga
     2761 gaatggccaa tactacgccg gtgaggtaga gcgaaaaaat gacgatgttg ccctgattcg
     2821 ggaaaaaggc catggcgaaa atcgcataga ccggcatgcg cgcgccgcag gacatgaatg
     2881 gggcgatcag catggtcagg agcctgtcgc gacgttgttc cagcgtgcgg gtggccagca
     2941 gggccggcac ggtgcagccg aagcccacga gcagggggat gaaggccttg ccgggcagtc
     3001 cgatacggtt caggaagcgg tccatgatga acgcggcacg cgccatatag ccggactcct
     3061 ccagcaggct caggcagaga aagatcatga agatcggggg catgaacgtg gcaaccgtcg
     3121 taaggcccgc gccgaccccg tcacagagca acgagcccag gatctcgggc atgcccagac
     3181 gggccagcca gatgcgcgga gcgttgacga gcaaggcgtt caacgccaga tccgtgaaat
     3241 cgaccagcgg ttgggtcagc cgcacggtca accagaaaac cacgtacatc acgccgagga
     3301 aaaacgggat gcccagcgga cggctgagca acagccggtc cgccacatcc gagacgcggc
     3361 gtcgcgagac tcgcttacgc tgaaccacgt cccgcgccaa gccgcggatg aatccgaggc
     3421 gcgcgtccat caccgcctct tcgggtttct gaccggtgtg gcgcgcgata cgggcgcact
     3481 cttcttcggc gagttcgtcg aacagccctg cggtcagttc gcgggcaaag ggatcgcgtt
     3541 ccagcagttt gatggccagc cagcgcgagt ccacacccgc atcatcggct gccacgatca
     3601 ggcagacctg caaacggcgc accgacggtt ccgcgtcgcc gtattgaacg cgggtggtgg
     3661 gcaggctgcg cgtctccgcc acatggcaga tcgcctgctt gagggcttcg agccccacgc
     3721 ctttagaggc gaccatcgga atcaccgggc acccgagatg gctggcgaga tgatcggcgt
     3781 ctacgtggat cccgtagcgt tcggccacgt cggtctggtt gagggcaacc accagcggca
     3841 ggcgcatatc gagtaactgg gcggtcaagt agagactacg ctcaaggttg acggcatcca
     3901 ccagattgac gatcacggca ggcttctgct ggatgaggaa atcccgggcc acacgctcgt
     3961 cctgcgtgtg cggagaaaaa gagtaaatgc ccggcaggtc ggtgacgcgg gccgttttgc
     4021 cattcagggt aaaggcggcg tgcgcggtat ccaccgtcac tccggtccaa ttgccggtgc
     4081 gttcgtgcgc tccggtcagc aagttgaaca gggtggtctt gccggtattg ggattccccg
     4141 ccaacgcaat ggtcagatcg tcacgcatgc ggcacgttcc ggttccaatt gttagccagg
     4201 gaaaacatgg gccgaattat atggggcgga acccccgagc acaagcgtaa tcgctggatt
     4261 tttgttgttg acggagggaa ggtgcggtgc gatggtataa acaaacgccc tacagggtgt
     4321 gtgccctccc aaaggagtca gaatctcatg tcccaaccgg ttcggatcat gccgtgtctc
     4381 gatatgcggg acggtcgcgt tgttaaaggc gtgcgttttg tggatatccg ggatgccggc
     4441 gatccggtcg cgtgcgcgcg ggcctattgc gaaaaggggg ccgacgagct ggcgatgctg
     4501 gacatcacgg caaccgtgga ggggcgggcc acgctgctgc aggtggtccg taacgtggcg
     4561 gaggccgtga cggtgccgtt tacggtgggg ggcgggattt cggatgtaaa gaccgcgcgg
     4621 gcggtgatgg aagcgggggc aaacaaggtt tccgtcagta gcgcggcgtt ccgcaagccg
     4681 gcgcttatcc cggagttggt cgccgaactg ggtgcggagc gggtgaccgt ggccattgat
     4741 gtggaccgga atgcggcgct gccctcggga tatgaggtgt acatcgacgg cgggcgtacc
     4801 gcgaccggtc acgatgcggt cgaatgggcc aagcgggtgg acgggttagg ggtgcgggtg
     4861 attctgccca ccagcaaagc gggggacggg gcgcgcacag ggtatgatct gccggtgatc
     4921 cgcgcaatcc ggcaggcggt ttcggcggag gtggtggcct cgggcggcgc cggaacgttg
     4981 gagcatttcc acgaggcggt tgaggccggc gccacgattc tgctggccgc ttcgatcttc
     5041 catttcggga tcatcggtat tccggaactc aaagcgtatc tgcgggaacg cggggtcaag
     5101 gtctgcggcg gcgaagcttg acgcgcgtgc cacgcggtcg gggaggtgta tgatgaaagt
     5161 ggtcgcattt aacggaagcc cccgtcagga cgggaacacg gcgctgctgc tgcgcaaggt
     5221 gctggcaccg gtcgcccgct cgggaatcga caccgagttg gtgcaggtcg gggggcacgc
     5281 ggtccacggc tgtaccgcct gcatgcgctg catgaccgcc cgcaaccggc agtgcgcgat
     5341 cacgaccgac agggtgaaca cctggatcgc aaagatgatt gaggcggacg ggatcgtcat
     5401 ggggtcgcct tcctacttct ctggcatgac gcctgaactc aaagcgctgc tggaccgggc
     5461 cgggctggtg gcggtggcca acgggcggat gttcgcgcgc aaaatcggcg cggccgtggc
     5521 ggcacaccgc cgcggcggcg cggtgaatgt ggtggacgcc atcaaccatc tgttcctgat
     5581 gtcacgcatg atcgttccgg gatccacgta ctggaatttc ggcgtgggct tggagcgtga
     5641 ggatgtggcg gacgacgcgg aagcgatgga gaacatgcac gatctcgggg agaccctcgt
     5701 ctggttgctc aagcggcttc acgcggacac ctgaagcggg gcgcgtgctc agaaaccaac
     5761 cccaaataaa gccagatcaa cggacgggac gcggctcttt tgtgcgaggt tcgagacttt
     5821 cgcggagtgc ggcgagatcg cgttgcagtt ggacaagttt ttcgtccagg gcggagattc
     5881 gcttgtcgag cgtggggtag gcggccagaa ccgcttcgtt cgccggatca tagaccagcc
     5941 ccagaaaggc gtagagcggg gtgaaatagc tgtcgatgtc cgcaagccgt ttttccattg
     6001 ccgccagacg ttcggcgaaa tcctcgggca cggccgtgac ggtgaccgtc tcctccagat
     6061 gctcgaacgc ttgatcgaga taggcggtga gttccgtcag cagatcgggc agggcgttca
     6121 ggtgtccaat cgcggcgcgg aattcgtccg tgcggagtgc ggcggggctg tgtggtgccg
     6181 cggaaccggt gggggcggcg cgcgtctcct tcggtgcccg ggattcgagg tgtgacaggc
     6241 ggcggttcag tgtggcgagc tggacattca gggtttccgc cttccgcaac aggtcagcct
     6301 ggcgctcccg taacccatcg ggttccgcaa gagcctgatg aaacacactg cggatccggc
     6361 ggccttcttc gcggattgcg cgttggaacg ccatgactgc ggccagcagc gccagaccga
     6421 gggccagaaa ggcaaccgtc tttctcatgc gcacctccgg gagtcagggc gttccgtctc
     6481 ggttctttac ggtgccgacc gcgcgacgga gtctcaagcc cgccagggac gctcgcgttc
     6541 ctgacaggag gggaagattt cgttgaagag attcacgttg tcgaccaact ggaaatcgta
     6601 catgccgacg cacaggaaat cggcaccagc cttgagcgcg aacgggaacc cgtctttagg
     6661 ccggatcgcg ccggccgcca gggtcttgaa cccgatccac ggttccggcc gctctttcat
     6721 gtaggcgatg gtctctttcg ggtttgtgca ccagatgttg tcgtggcgcg tgccgccgtt
     6781 gatgttcgct gaccagtagt tgatcggatg gatggttttg acccagtagt cgggaatcag
     6841 accgcggtcg acgcactctt tgaccgcctc cagactgtgg gcgccgatac ccgcctgata
     6901 gccttctttg cgcatgaagg cgagggcgtc ttccatgatc ttgtagttct tctcccgaat
     6961 gatgtatttt tcggtcatct ccccttggat gtacaacatc tgcgcgccga atttgatcga
     7021 aatctccgct cctttgatgg gattgccgcc ggccgcgcaa tcggagagca cctgcatttt
     7081 gccgccctct ttcttcagat aatcccccat cacgcggaag agtccgggat tcaggatgat
     7141 ggtgttcatg ccggctgctt ccgccatgtg gagcgtgcgg aagacgcgcc agtcctcgtg
     7201 gtaggccttg acgagttggc tgacgtagat caggtcgcgc gcatgcgccc agccgccgat
     7261 caggttacac cccatgaaca tgcggctgag ccgggtcgag ccgatctgcc cgaaggcttc
     7321 gatcggcttc ttgagtttgc tgatgtcttt ggattcaaag gtgcggagcg tgggaccggt
     7381 catggcgtcc gcgatgcttt tcagatgctc tttctcccat accgggccgt gcttctctat
     7441 gaaggcgcct gcaaaggctc ccagaacggg cagggaactg aggttcttga ggaattcgcg
     7501 ccggtcgaat gcgatcgggc tttcgcgtcg attccggtac tgctggaaca gggcgcccaa
     7561 cccgtaagag gggatcacgc aaatcaccgc cagcgcgaag gcttcgatga cattccggtc
     7621 gatccacaga agatgggatt cgccgcctgc gggcgcggtg aagggaggat gggccagata
     7681 gtacaacgcc aggaggacga tgccgctaat ggatgcgaac cggatggcga ctcccaaaat
     7741 gagggcgagg ccgatcaggg tcaatcccca gatgttcagg aggtcgacga ccttcagcag
     7801 cgccggcgtt tccgcgatcg tatgaaacag tttcgcggcg atccattcgc tggaactcag
     7861 gtaaggttcg gcgctccagc ttccggagag caacttccag aggccctcat ataagaaatg
     7921 ccagccgacc agaaaccgta gcaaggtgac ccagtaggat tttttcatgt tttccctttc
     7981 tgtctgtctt tccgttcgtt aaaaaaccac cgttgcgctg accgggaaat gatcggaggg
     8041 gtagttcccg ttttccgcgt cgttgtgcgt ggtgacgtcc cgcaccgtca ccttttcaga
     8101 aacgaagatg taatcgatgc gcggcgcatc tttggcgggg gccttcggct cttcggcgtc
     8161 aggccggtac cggtacccgg taaaagtgcg ccacggacct tgatgcgggg tttgcgtgtg
     8221 gcggacggca tcccgcaaca cctttccggc caactggacc ggttcggagg tttcgttgca
     8281 gttcatgtcg ccggtcagga agatcggcca tccttgcggc acgagcgtct gcatacgctc
     8341 cagaatcagt ttcattccgt tggcgcgggc ttccacgctg atatgatcca gatgggtgtt
     8401 gaagaacgcg aacggtttgt tgtttttcgt gtcggtcatc agggcccagg tgcagacacg
     8461 cgtgcaggcg gttttccagg acttcgaccc cggcacctcg ggcgtctcgg agagccagaa
     8521 ggttccgctc ttctgaaccg tgaaccggtc agcccggtaa aggatgcaag tggcttcgcc
     8581 tttacgcttc ccgtcatcac ggcagacgcc gaccatggcc cagccgggaa gcgcggcaag
     8641 catttcgtcg aactgcatag cggttgcctc ttgcaaaccc accagatcga atccgtgccg
     8701 cttgaccagc gcgatgacgc ggggggcgcg gttgctccaa gcgttgacac ccttatccgc
     8761 cggaagccgg atgttgaagg tcgcgagacg caacggctcc gcaaccgcgg aatggcatgt
     8821 catgaacccg gccaacaggc acaagtataa agtgcggatc ctcatcgcaa gttccccctg
     8881 atcgtgatcg cgtaatgtca catcatgcca caaacgctac cgacctgtca aaaaagcaat
     8941 cgagcgaatc acaacaacag cgcggcaaga aggcggacat tctcgtctga ggatgcggcg
     9001 ggcagccgct ccgacgctgt ggcgcggtct ccctcaaaaa cggcgcggca ccagacgccg
     9061 acggcatcgg tgccaaaggc cgcggccacg gcgtccgcct tcgcgctctg cccgcgttcg
     9121 cggcacgcca gcgcggtcag caggtccagc gcatttgcgg tgcgcgccgg acgactgggg
     9181 gcgaatcccg ccgcgacccg gtcgagaagg tctgcgcggg tgacgccctg ttcaagttgc
     9241 gcgaagagcc agagttcgag gcgcatgtcg atctcgctcg catagggctt gccgacgccg
     9301 agtttctcgt cccagatgcc ggcgcgccgg accgcatccc gcgtaacggc gctgtcgctc
     9361 ttgcggaacg cggcgaccgc ctgcgcgagg caggcttcgc gatacaccct gcgggtgatc
     9421 gaggtgaact ctgacggcag gaaccgtacg gtgtcgaggg tttcgagaca gcgggcggga
     9481 tcacccagcc gcagatggga ctgggccagc agaatgcctg catcgttgtc ttcggggtgg
     9541 gcggccaaca accgttgcgc ggcacccaga gcctgccgcc agtcattggc ttcggcatgg
     9601 gcgcgccatt ccgcttttac agcgtaccgg ttctcgccga acgtctgggc cttccggatg
     9661 tcggccagcg cggacggccc gtcacgcagg cgcgcgcgga aaagcaggaa cggcacgtca
     9721 tcggcatcgg tctcgttcaa gagggcccgt gcccggtcgt cgttaccgaa atggcgcagg
     9781 agcagggcga gcgcgtaacg cggtttccaa gagggggaat gggcgtgcgc ccattcaagg
     9841 atcgggaggg tctccggccg gaagggaaag gcaaaggcgg tatccagccg ctccgcgtcg
     9901 gccagcgcgt tccgggcggc ggtttcatcg ccggtccgcg ccagccaata cgcgcgcgcc
     9961 acggcagcga ggaagcgtcc ggacgcgagc gcggcgatct ccgcggcctc gccgaacaga
    10021 ccggcggcgg cgtacaggtg cgcggtttcg aggatctcct cgtgcggcaa ttcgcaacgg
    10081 atccccgcag caaaggcggc acggtcctgt tcgcaacgcg ccggatggaa gagcggaaaa
    10141 cggtcgagca ccgcagcgca ggctgcccgg tgttcatcct cccgccccaa caggcggagg
    10201 gctacgaggc gcaggtgcag cgcggaaaac tccaaggggt tcgcttccag tgcgcgctcg
    10261 gccgcctcca gcatccggtc gggcctcccg gcggcacact ccagcgcggc gatccgcgtg
    10321 atggcggcgc tgcggaaaag cggcgacagc gcggccgcgc cgaaccgttc gagggcgttc
    10381 gcgtgatccc cgcgctccgc gaagacgatt ccgctgaggt ggttcgcctt gtcgtgatag
    10441 gcgtcaacgg ccagcgcacg ctcgctgaag gcgagggctt cggcgggacg gccgcagtgg
    10501 agcagcaggt cggccatgca gaccagcgcg gggaggtaat gcggttcttt cgccagcacg
    10561 gcgcgcaacg ttttctccgc cgcagtgtag tccttgaggt tcacatcctg ttccgcctga
    10621 agcgtctggc cgtaaaccgt gccccagtcg aagtcggcgg gcgtcgcgag cgggcggctg
    10681 accgtaccgg attccgcggc gcgctcctcc agaatccggg cctcggttac cggtgaccaa
    10741 cgctcgctga aggtgatcgt cgaaccggcg gggaaggagg ggtatttgaa cggggtgcgg
    10801 tgactgccat gggacggctg gttgaaatag cggcccgact gcagctcgac atattggccg
    10861 tcctcatcgg tgaggagatc ctcccagatg gcaccggaac gcgacaggct ccaaatccag
    10921 attttgcgtc caagccggtc gaagcgggca gcctcatgca tggaaccgaa ctggtgcgcg
    10981 tcccaccaga cgccgaagaa ccccgtgtcg ccgttgacga catgatagct cttcgacagg
    11041 ccgaaggcgt tgttcgtata aacggaaaga tccagtccgg aagcgtcccg cggccacggt
    11101 gaggctgtcc cgtcatgttc gagccgggcc gtacccggaa agacatatcg gccgccacac
    11161 ggataggcgg cgttcatcca ctggtaacag ggttgcggca ggccggaggc gttgtgccag
    11221 gtggtctgcg tggtgaaaaa gcggtcggag gcgccgagac ggatctcgac gcaccagcgg
    11281 gtgcgcgtga tgagttccag cacaccgaca aaacaacttg ccgaaccgtc ggagttcgtg
    11341 cggcaggcgt aatccacggg ggtcgaggcg gtcggcgcat gtccgagcat gccgaagttg
    11401 aactcgatgc ccccgctggt ccagggtccc cgcgtggaga tgttgcggaa cttgacgacg
    11461 cggttacgat agaggaagtc ccgtccggtc cgcttgtccc gcgcgctcca gaccttcccg
    11521 ccgatttgcg gaaacacatc cacctcgacc gcgtcgtttt ccagccggac gcaggtccag
    11581 gattgcgttg tggcaaccgc cgtgtacccc tcgaatttga agtacggcca gatctggcgg
    11641 tccactctgg tttcgggttc cggaatcggg ttcggtgccg agaacccgta agtgggcatc
    11701 tccagcgtga tttccttgac gcgtgcggca ccatgcgaaa agcaagggaa ggccacgccc
    11761 gtgaaaccgg tcagcagaaa aagcgttgtt gccgtcagcc gggcgggttg tgatatcgcg
    11821 ccccttcgtt tatcctgtac attcatggcg aatcctttgt tatatgaaca gtctatcaga
    11881 gcgacggccg gtttttcaat tatgggatgt tt
//
LOCUS       DAOSLE010000080        11846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5409_length_11846_cov_7.244084, whole genome shotgun sequence.
ACCESSION   DAOSLE010000080 DAOSLE010000000
VERSION     DAOSLE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5409_length_11846_cov_7.244084"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK576_06765"
     CDS             <1..614
                     /locus_tag="PK576_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61331.1"
                     /translation="MSRVDFGWWYFFSPSGGARPTVGTQMDMWEYGASVAAAWDCAIS
                     ILMPTSALKRHPRTDDILEMMRRWGDCRRRGLFKDEWRPRLKNYAQEHHLIVDAKGNY
                     DLVDYSQIVVGNGQNPVRAFVFAKDNATWVVYWHGSGEGRLRLPIAAGAATLHDDFAG
                     KAVAFEQDGASIIVPATKRLYLKTTLTRDAVERAFAAATLLSK"
     gene            complement(1184..3904)
                     /locus_tag="PK576_06770"
     CDS             complement(1184..3904)
                     /locus_tag="PK576_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61332.1"
                     /translation="MKVLFMCLGLLACGCRFADKAFAEEQADFWVAPDGKDTDPGTKE
                     RPFATLARARDAVRALRQAEPDRDILVLFRSGTYPIEGPIGFSPEDSGRAGGRITYAA
                     APNATPVISGGRRITGFEPCGDGIWKTTVPNARDGKWRIEQLYVNGSRAVRARTPNGH
                     YAYIDSIVETGIDPATGKAGPIRDRAFLGRPKDIAPLLKLPQERLRDVVVSAYHSWEI
                     SRHRIVSIDPGRNLVLLTGRYPPKFGHYTSRERYYLDNFREALDAPGEWFLDRDGTLF
                     YKPRSGEDMAQAEVVAPVTETFLRFAGSAGGAQVENILFRGLTFEYAAYLMPHEGAWS
                     PQAACNIDAVVMADYARNIAFTDCAIRRTGTYGFWFRDGCRDCVVERCLLEDLGAGGV
                     RLGPTAHRPHDAGQLTSHCRIDNNIIRDAGRVWPDAVGVFIGHSGDNQVTHNDIAGMP
                     YSGISVGWRWGYGEVPSKRNTISYNRIHHLGWDVLSDMGGIYTLGEAPGTVMSGNVIH
                     DFSGDGECSMHGIYNDNSTAYMLMESNLVYNVRDGGYKLGSGKANIVRNNIFVSRLRD
                     KPRHSGPIMFCQYYTNETHVAATIEKNIVCGCEGQIFASSEFANRLELRNNVYWEPSG
                     TPIDFIGRSFEAWQKLGRDAGSVIADPKFADADNNDFHLQADSPALALGFVPFDSSRA
                     GVYGDPAWIAKAREFAVPPPWQGLPPPPPLSFSDGFEHTDVGSLPRRATSMVENKGDG
                     IEVTEETAATGKRSLKITDAPGLKHGFNPHFWYTPNHHEGVTTFSFDVHMEQGAELSL
                     EWRQYPGKPYYYIGPKLLFRDGKLTFAGQPPLPLPLGVWTHIEISAGLGSKTDGTWQL
                     TVTPQGAEPKRFSGLKFGNPETRSVTWIGFISVANRKGVYYVDNVKLENN"
     gene            complement(3901..>5972)
                     /locus_tag="PK576_06775"
     CDS             complement(3901..>5972)
                     /locus_tag="PK576_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61333.1"
                     /translation="QVIATIDPYSEKPCAAIGETKVGEIPGCRFRETGIKSHDRFAYV
                     RELPATNLLLRVRITYPDDTYRSLMVSQTQPDFGEIRTAGSEQQVLGSGILTGSEFPV
                     TGKPLVYEYVFRHPSTWLGLIFEGYQPDKPGAVGPVTIAYAAPEKDRYGPAAPLPSLA
                     AHKARKAGLYWEDPVLSMCFGGVGGVDPATYDRDLVSAMDYFSWVGQDLWFYPTVWYN
                     GPVYRSRKEKGTWPSGMRHHPADFPHLFAARCAERDITFVPSFGMSRLPTLNLKFGTR
                     EQVIAGDQTVLNVVYADGEIPVTPFMYKPPIFNALRRETQDAVMGLVDEHCDMLADLP
                     SFGGISFYLNHWNLTQLGLWLDGSYDDWTMETFAKGIGEILPGTPGDAQRFKIRADWI
                     RNDPARRNRFLRWRAEIVSGFYEKVAARIRSRNSDAKLYLRLDVPSISQMCTSGGRFC
                     KPFSDESFLEMGLDVPRLAKHEAVCLDRLFSWGIVRCERKYWPDEKLVPPLFDLEPAF
                     QKEVLGAVDAVTIHQYYFETHGEFSRGRKLTMPAPWKNEAPGRCTAPLYPGRWPLVYY
                     ARALALFDMKEVSIGGFTLGTHGIEDLVRTWTASFRSLPLVDFGEEVRFDDVILRTAV
                     AEGARWWYMLNTASQERVIPLSVKGVCRETVYGDRVKSGSRTLKSFELVAVRGEMDSQ
                     LTIKETP"
     assembly_gap    5973..5982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5983..6596)
                     /locus_tag="PK576_06780"
     CDS             complement(<5983..6596)
                     /locus_tag="PK576_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61334.1"
                     /translation="MAAFCCVGSATFAAVAPGVYPADEVPVARGSIELWVTPNQDGCD
                     ARDHVYFADADAWAREGTPTIWYWAEYVRFDVDSGKRLVYGRLADGLLEPDVPVHIVA
                     SWDAAKGQTAVYANGELVQRREVEPWVPVALERYVVGEDCSRRKKAGAVIDALHFFAV
                     PLTPGEIKTRFDAKKNLVDSARSSAARKKHMPPPLPAKATVAPE"
     gene            complement(6636..10913)
                     /locus_tag="PK576_06785"
     CDS             complement(6636..10913)
                     /locus_tag="PK576_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61335.1"
                     /translation="MKRIAFFSLVMLGGAFAALGNQWAAPVSGAFNDATKWNPAAVPD
                     TVNGQAWWYNAAQGKYTVTLPEGTWTNPVAWRVDANTDGTEITFCGSNTVWAPPTYAT
                     AIYQGEPFAFYDQYSHFFSFEMYNDKRGAPFKFTNPVFHAYNQDGVAGIIFHSGEFNF
                     HDPSGTAHNYDWTVSHGNDAATLAGAVHRVEVRNGVTAKVANINVRSRAARTELVVDG
                     GVLTAAANLNIPSGGGGDSRTAVDVTVTDGGIFNIARMTLGYAGKPRDIHFVVTNNAR
                     MVFSESQDYKFSGVSNGADFDFDVTDGGVVEVGHELGLGWSANAPTRVRVLNSSWLVS
                     KETKIGNDIAGVDCVFAATNSLIDSYRGNDSAQFRVNGEMILKDCVWTNALVNAWPAN
                     TDPKITIEGGAWTSLKKFHFGGGADAGRLTVNGGQHDLRGEFYLGYNPNANVPTKSVA
                     TIRRADTVIDFNSDEMIALGWNGTADMIIEDGVLRFSGAGGLSLGHAGASSTGSVRML
                     KGTLDASVPNLGVCVGRNGFGTFTQEGGAITVRQMFMGQVAVSDADLTCVFTQKGGTF
                     TALDSIGITLAYAQGRKTKLVLDGGVMSVPQIIGGTGAAVNGGTGLALLAANGGTLVA
                     RRDNATFVHALDGVELGEQGLVVDTDYDVTITAAISSKSNEKGVLVKRGRGTLTLAGT
                     VADTVKVEVQGGKVVFGQSVTLDALTLGTENSSGAIELADGVTLAITGAVTINHNASM
                     QEFVFEEDLNGVTHVTVDDSPAETLEIRLDEAVTSNATAAVEFRAKDTLNVSVVSGGT
                     LNLGGKLTRGALVTKGGGHAVLQNTGNEFYGGVTVESGWLSFTDARALGLTFSDAATL
                     TLKGGTFEYMGTTASAIPSKLSMASAVTTNPIVVKTSGDMTFGPFSAAGGALIKAGPG
                     KVTLAAKNGNTVVTSGAGIGTGPNGCPRYGFALDPNGGASAGEYAALNVVEGELVLQG
                     AGNVKSAGGVVAIGLNAAEGVAQPILTVDEAYLDNNQHFFVGGNIGASAFATNAMLQI
                     TNGGTVKCDTLRVGTMTSGKPSYPEVRVNGGTLDCGFRLNCADEQTWTRARYLFENGA
                     TLKIGSYNYSSWQLYWEGEAAFTFDASSYAIAGNNHYADVHVRQAGRGSMLFRNNSTF
                     YGEKITKWSGDNYKLTFAFDNGEWIPGTGDYDFTFTGGLGIIVDCRAGGLTLNVPATK
                     TWTVGQKISGTGGIVKKGTGTLAFTPQATIVEGARTLVADDPVSWAFADTLKVESGTL
                     AVANDAATPFASAELAAGTVFNPGSALVFGEVSGSGTISGGGLVATTLRAAELATLTL
                     SGVTLTSPTFKPAVDASGTLTGHFVLTNCDYDGQVIVDFGRSELDPLDAGTTYEIGTV
                     TGTLPDLARWRVVNTGIAQGAGDFSPVGEDGKIRVTLSRRGFLMTVR"
     gene            complement(11623..>11846)
                     /locus_tag="PK576_06790"
     CDS             complement(11623..>11846)
                     /locus_tag="PK576_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61336.1"
                     /translation="YTPGTEKAVDFCFSDHAFRISPGDVLRLDVSSSCSYFVPHTNNK
                     GLQSVQKTSRVANNAIICGKSSVTLFCEQ"
BASE COUNT         2562 a         3710 c         3187 g         2377 t
ORIGIN      
        1 acatgtcgcg cgtggacttc ggctggtggt attttttcag cccttccggc ggcgcgcggc
       61 ccacggtggg cacccaaatg gacatgtggg aatacggcgc ctccgtggcg gcagcgtggg
      121 actgcgcgat atcgattctc atgccgacat cggcgttgaa gcgccacccg cgtacggatg
      181 acatccttga gatgatgcgg cgctggggcg actgccgcag gcgcggcctt ttcaaagacg
      241 agtggcgtcc gcggctcaag aactacgcgc aggagcacca cctcatcgta gacgccaaag
      301 gcaattacga tctcgtcgac tactcccaga tcgttgtcgg caacggccaa aacccggtgc
      361 gcgccttcgt tttcgcaaag gacaacgcca catgggtcgt ctactggcac ggctcgggcg
      421 agggacggtt gcgcctcccg atcgcggcag gcgcggcgac cctccacgac gatttcgccg
      481 gcaaggctgt cgcgttcgag caggacggcg cgtcgatcat tgtgcccgcg acgaaacgcc
      541 tttacctcaa gacaacgcta acacgcgacg cggtcgagcg ggcctttgca gcggcgacgc
      601 tgcttagcaa gtaggattga ggatttagga ttgaggattt agcccgccgc gcggctctgc
      661 cgcgaaaaaa ccttggatgt gagtttagga catgatttcc gacagattcg cttcggcggg
      721 gagaaatcca tgattaacat gatgttgttc aatcctgaaa atcctgtcat cctgtcaatt
      781 tataggcaag gagtttaacg cagaaggcgc agaggttttt ggggggacag ggctggccgc
      841 aagtcgcaag tcgcgagtca acctctctta ttgcggccac gtagtggctt gcagtcgtga
      901 gagtaaaagc actgctgcga agaactggac tcgggcgtgc tgattcgctt tatcgagcaa
      961 gacggtgagg cacacagaga gcggatgcgc gagaccgcct cgcatttcgg attcagggca
     1021 ccccaattgc ccgacttggt ggctcggcta tcggccgagt tgcgaaacaa gagagtgaag
     1081 cggagatcac ggtgagcgac cttcacctct tcaagaccgt acacctcatc aatgctatgc
     1141 tggacatcga agacggggtt gctctactcg tggtgaacgt cggttaattg ttctctaact
     1201 tcacgttatc gacgtaatac acgcccttac gattcgcgac gctgatgaac ccgatccatg
     1261 tgacactgcg agtctcggga ttaccgaact tcaggcctga aaaacgtttg ggctccgcgc
     1321 cctggggggt caccgtgagc tgccaggtgc cgtcggtttt tgagccgagg cccgcgctga
     1381 tctcgatatg cgtccagacg ccgagcggca ggggcaacgg cggttggccg gcgaaggtca
     1441 gtttcccgtc acggaacaac agcttcggcc cgatgtagta gtacggcttg cccggatatt
     1501 gacgccactc aagactcagc tccgcgccct gctccatgtg gacgtcgaag gagaaggttg
     1561 tcacgccctc gtgatggtta ggagtgtacc agaaatgcgg gttaaagcca tgcttcaatc
     1621 cgggtgcatc ggttattttc agcgaccgct taccggttgc ggcggtctct tcggttacct
     1681 caatgccgtc ccccttgttc tcgaccatgg aggtagcgcg tctgggcagg ctaccgacat
     1741 ctgtgtgttc aaagccgtca gagaatgaca acggcggagg cggcggcaac ccctgccacg
     1801 gcggcggcac ggcgaactcg cgggccttgg caatccaagc gggatcgccg tagacgccgg
     1861 cccgcgaaga gtcgaacggc acgaagccga gagccagcgc gggagagtcc gcttgcagat
     1921 ggaaatcgtt gttgtcggca tcggcgaact tcggatcggc gatgactgaa ccggcatcgc
     1981 ggcccagttt ttgccacgct tcaaacgagc gcccgataaa atcgatgggc gtgcccgatg
     2041 gttcccagta tacattgttc cgcagttcaa gccgattcgc gaactcagag gaggcaaaga
     2101 tctgcccctc gcacccgcaa acgatgttct tctcgatcgt agccgcgacg tgcgtctcgt
     2161 tcgtgtagta ctggcagaac atgatcggac cgctatgccg tggtttgtcg cggagtctgg
     2221 agacgaaaat gttgttgcgc acaatgttgg ccttgccgct gcctagcttg tatccgccgt
     2281 cgcgcacgtt gtagaccaga ttgctctcca tgagcatgta ggcggtgctg ttgtcgttgt
     2341 aaataccgtg catgctgcat tctccgtcac cactgaagtc atggatgacg ttgccgctca
     2401 tcaccgtccc gggcgcttct ccgagcgtat agatgccgcc catgtcagac agcacatccc
     2461 atccaaggtg atgaatcctg ttatacgaga tggtgttgcg ttttgatgga acctcgccgt
     2521 aaccccagcg ccagccgacg gaaataccgg agtaaggcat tcccgcgata tcgttgtgcg
     2581 tgacttgatt gtcgccgctg tgcccgataa aaacaccgac ggcgtccggc cacacgcggc
     2641 cggcatcgcg gatgatgttg ttgtcgatac ggcagtggct ggtcagttgc ccggcgtcgt
     2701 gcggcctatg cgcggtcggc cccaaccgca caccgccagc accaagatcc tccagcaggc
     2761 agcgctccac gacacagtcg cggcatccat cgcggaacca gaacccgtag gtgccggtcc
     2821 gccggatcgc gcagtcggtg aaagcgatgt tgcgcgcgta gtctgccatc acgacggcat
     2881 cgatgttaca ggcggcctgc ggcgaccacg cgccttcatg cggcatgaga taagcggcgt
     2941 actcgaaggt caacccgcga aacaggatgt tttcaacctg cgcacccccc gcgctgccag
     3001 cgaaacgaag aaaggtttct gtcaccggtg ccacgacctc cgcttgggcc atgtcctcgc
     3061 cggaacgcgg tttgtagaag agcgtgccgt cgcggtcgag gaaccactcg cccggcgcat
     3121 ccagcgcctc gcggaagtta tccagatagt agcgctcccg cgatgtgtag tgaccgaatt
     3181 tcggcggata gcggcccgtc aggaggacta ggttgcgtcc gggatctatg ctcacgatgc
     3241 ggtgccgtga aatctcccag gagtggtagg cggagaccac gacatcgcgc aaccgctcct
     3301 gcggcaactt taacagcggc gcgatatctt tgggacgccc cagaaacgcg cggtctctga
     3361 tcggcccggc cttccctgtc gcggggtcga taccggtctc cacaatactg tcgatgtagg
     3421 cgtagtgacc gttcggcgta cgggcccgca cggcgcgact gccgttgacg taaagctgct
     3481 caatgcgcca cttgccgtcg cgggcattcg gcaccgtcgt cttccagatg ccgtctccgc
     3541 aaggctcaaa cccagtgatg cgccgtccgc cgctgatcac gggcgtagca ttgggcgcag
     3601 ccgcataagt gatgcgaccg ccggcacgtc ccgaatcctc cggtgagaaa ccgatcggcc
     3661 cctcaatggg atacgtacca ctgcggaaca agacgaggat gtcgcggtcc ggctccgcct
     3721 gacgcagcgc gcgcacggca tcacgcgccc gcgccaacgt ggcgaacgga cgctccttgg
     3781 tgccgggatc ggtgtccttg ccgtccggcg cgacccagaa atcggcttgc tcctcggcaa
     3841 acgccttgtc ggcaaaacgg cacccgcagg ctaacagtcc caaacacatg aacagaacct
     3901 tcatggcgtc tccttgatag tcaactgtga atccatctct ccgcgaaccg cgactaactc
     3961 aaaagatttc aacgtgcggg agccggattt cacacgatca ccataaaccg tttcacggca
     4021 cacacccttg accgacaacg ggatcaccct ttcctggctt gctgtgttca gcatgtacca
     4081 ccagcgtgcc ccctctgcca cggctgtccg caaaatcaca tcgtcgaaac gcacctcctc
     4141 gccaaaatca acgagcggta aactgcggaa cgacgccgtc catgtccgca ctaaatcctc
     4201 gatgccatgt gtacccaacg tgaaaccacc gatcgacacc tctttcatgt cgaagagcgc
     4261 taaagcacgg gcataataca cgagcggcca acgtccggga tagagcggtg ccgtacagcg
     4321 tcccggtgcc tcattcttcc acggcgcggg catggtaagc ttgcgtccgc gggaaaactc
     4381 accgtgtgtc tcaaaataat attggtgaat cgtcaccgca tcaacagccc ccagcacttc
     4441 tttctgaaac gccggctcta aatcaaagag cggcggcacc aacttctcat ccggccaata
     4501 tttgcgctca caccgcacga tcccccaact gaaaagccga tcaagacaaa ccgcctcatg
     4561 cttcgcaagg cgcgggacgt ccagtcccat ttcgagaaac gactcatcgg aaaacggttt
     4621 acagaaacgc ccgccggatg tgcacatctg ggatatggaa gggacatcca aacggagata
     4681 cagtttcgca tctgaatttc gcgagcgaat ccgcgcggca accttttcgt aaaagccgct
     4741 cacaatttca gcgcgccaac gcaagaatcg gttccggcgt gccgggtcat tcctgatcca
     4801 atcagcccgg atcttgaagc gttgcgcatc acccggcgtg ccggggagga tctcgccgat
     4861 tccttttgcg aacgtctcca tggtccaatc gtcgtatgag ccgtccagcc acaaccccag
     4921 ttgcgtgaga ttccaatgat tcagatagaa tgaaattcct ccgaacgacg gcaaatcggc
     4981 aagcatgtcg cagtgttcgt cgacaagccc catcaccgcg tcttgcgttt cgcgacgcaa
     5041 tgcgttaaaa atcgggggct tgtacataaa tggcgtcacg gggatctcgc catccgcata
     5101 aacaacgttc aataccgtct ggtcacctgc gatcacctgt tctcgcgtgc cgaacttcag
     5161 gttcaatgtc ggcaaacggc tcatcccgaa actgggcaca aaggtgatgt ctcgctccgc
     5221 gcaacgcgcc gcgaagagat gcgggaagtc cgccggatgg tggcgcatcc cgctcggcca
     5281 tgttcccttt tccttgcgag agcgataaac ggggccgttg taccacaccg tcgggtagaa
     5341 ccacaggtct tggcctaccc acgagaaata atccatggcg ctcacaagat cacggtcata
     5401 cgtcgcagga tcgacgcccc cgacgccacc gaagcacatt gaaagaacag gatcctccca
     5461 atacaggcca gccttgcggg ccttatgcgc cgcaagcgaa gggagcggcg cggccgggcc
     5521 gtaccggtct ttttcaggcg cggcatacgc aatcgtaacc ggccccactg caccgggctt
     5581 atccggctgg tatccctcaa agatcaaccc caaccacgtt gacgggtggc gaaacacata
     5641 ctcgtagaca agcggcttgc ctgtgacggg gaactcgcta cccgtcagga tgcccgaacc
     5701 caaaacctgc tgctcggaac ccgctgtacg gatctcgccg aaatcaggtt gcgtctgcga
     5761 caccatcagc gaacggtagg tgtcgtctgg ataagtgatc ctcacgcgca aaagtaaatt
     5821 cgtcgcgggt agctcacgta catacgcgaa ccggtcatgc gattttattc cggtctcgcg
     5881 aaacctgcat ccgggaatct cgccaacctt tgtctcgccg attgcagcac agggtttttc
     5941 cgaataggga tcgattgtcg caataacctg ccnnnnnnnn nncactccgg cgcgacagtt
     6001 gccttggcgg gcaatggcgg gggcatgtgt ttttttcggg cggcagacga gcgcgccgaa
     6061 tcaacaaggt ttttcttagc atcaaatcga gttttgattt caccgggtgt caacggcaca
     6121 gcgaaaaaat gcaacgcatc aatcaccgca cccgcctttt tcctgcggga gcaatcctcg
     6181 ccgacaacgt atcgctccaa cgcgacggga acccacggct cgacttcacg ccgttgcacg
     6241 agttcaccgt tcgcgtacac tgcggtttgc cccttcgccg catcccaaga tgccacaata
     6301 tgtacgggca catccggttc aagtaagcca tcggcaagtc ggccataaac cagccgcttg
     6361 ccggagtcta cgtcgaagcg cacatattca gcccaatacc aaatcgtggg ggtaccctcg
     6421 cgcgcccatg cgtccgcatc tgcaaaataa acatgatccc gcgcgtcgca cccgtcctga
     6481 ttcggcgtaa cccacaactc gatcgatccg cgagcaacag ggacctcgtc cgccggatac
     6541 acgccaggcg caaccgctgc aaatgtcgca ctgccgacac aacaaaacgc tgccactgtc
     6601 ccccaaaacc gggttctcac caagcgtcct ccgcctcagc gcacagtcat caggaacccg
     6661 cgccgcgaca gggtcacccg aatttttccg tcctctccca ctggcgaaaa gtccccggcg
     6721 ccttgcgcaa tgccggtgtt gacgacccgc caacgcgcga ggtcaggaag agtcccggtg
     6781 acagtcccga tttcgtaggt cgtcccggca tccaacggat ccagctcact tcttccgaaa
     6841 tcaacgatca cttggccgtc gtagtcacaa ttcgtcagga cgaaatgtcc cgtcagcgta
     6901 cctgatgcgt ccaccgcagg cttgaacgtg ggcgaagtca acgtcacccc cgacagcgtc
     6961 aatgtcgcaa gttcagcagc gcggagggtt gtcgccacca gaccaccgcc ggatattgta
     7021 ccgctgccgg aaacctctcc gaacacaagc gctgaaccgg gattgaagac cgtccctgcg
     7081 gcaagttcgg cgctggcgaa cggagtcgca gcatcgttcg cgactgcaag cgtaccgctc
     7141 tccactttca gcgtgtctgc gaacgcccac gagaccggat catctgcgac cagagtgcgc
     7201 gctccttcga cgatcgtggc ctgcggcgta aacgcgagtg tgccagtgcc ttttttgacg
     7261 atccctcccg taccggaaat cttttgtccg accgtccacg ttttggtcgc tggcacgttc
     7321 aacgtcaatc cgcctgcccg gcagtcaacg atgattccca aaccgcccgt gaaagtgaag
     7381 tcgtagtcac cagtcccggg tatccattcg ccgttatcga aggcaaatgt cagtttgtaa
     7441 ttgtcaccgc tccacttcgt gatcttttca ccgtagaatg tcgagttgtt ccggaacagc
     7501 atcgatcccc gccccgcctg gcgcacatga acatcagcgt aatgattgtt gcccgcaatg
     7561 gcgtacgagc tggcatcaaa cgtgaatgcc gcctctccct cccaatacaa ctgccaagac
     7621 gaatagttgt atgagccgat tttgagcgtt gcgccgttct caaaaaggta gcgtgcgcgc
     7681 gtccaagtct gttcgtccgc acaattcagg cgaaatccgc aatcaagtgt gccgccgttc
     7741 actcgtacct ccggatagga gggcttgccg gatgtcattg tgccgacacg cagcgtgtcg
     7801 cacttcaccg tgcccccgtt ggtgatttgg agcatggcgt ttgtcgcgaa ggcgctcgcg
     7861 ccgatatttc cgccgacgaa aaagtgctgg ttgttatcca gatatgcttc atccacggtc
     7921 aatataggct gcgcaactcc ctctgcggcg ttgaggccga tcgccaccac accaccggca
     7981 gactttacat tgccagcacc ttggaggacg agttcacctt caaccacatt cagggcggca
     8041 tactcgcctg ccgacgcacc gccgttcgga tctaatgcga aaccgtaccg cgggcatcca
     8101 ttaggacccg tccctatccc ggcaccagac gtcacaaccg tgttgccgtt cttcgccgca
     8161 agcgtgacct tgccggggcc ggccttgatc aatgcaccgc ctgcggcact gaacgggccg
     8221 aaagtcatgt cgcccgaggt cttcaccaca atcggattcg tcgtgaccgc agacgccatt
     8281 gacaacttgg aggggatcgc tgatgctgtt gtccccatgt actcaaatgt gccaccctta
     8341 agcgtcaacg tggcggcatc tgaaaatgtg aggcctaacg cacgcgcatc ggtgaaagag
     8401 agccagccgg actcgaccgt cacgccgccg tagaactcgt tacccgtgtt ctgaaggact
     8461 gcgtgtcccc cacccttcgt gacaagtgcg ccgcgcgtga gtttcccgcc cagattcaac
     8521 gtgccgccgg acaccaccga cacattcagc gtatccttcg cacggaactc gactgccgcc
     8581 gtcgcgttgg atgtcacggc ttcatcaaga cggatctcaa gcgtctcggc cggcgaatca
     8641 tcaaccgtca cgtgcgtcac accgttcaag tcttcttcaa aaacgaactc ctgcattgag
     8701 gcgttgtgat tgatcgtcac ggcgccggtt atggcgagcg tcacaccgtc tgccagttcg
     8761 atcgcgccgg aggagttttc ggtgccgagc gtcagggcat caagcgtaac cgactggccg
     8821 aacacaacct tgcccccttg cacctcgacc ttaaccgtgt ctgccacagt tccggcgagc
     8881 gtcagtgtgc cgcgtcccct cttaactaag acccccttct cgttgctctt cgaagagatt
     8941 gccgccgtga tcgtcacatc gtaatctgtg tcgacaacca gcccctgctc accgagttca
     9001 actccatcca gcgcatgcac gaacgtcgcg ttatcacgtc gcgccaccaa cgttccgcca
     9061 ttggcggcga gcagcgcaag gcctgtaccg ccattgaccg ccgcccccgt gccgccaata
     9121 atttgcggca cgctcatcac gcctccgtcc agaacgagtt tagtcttccg cccctgcgcg
     9181 tacgcaagcg tgatgccgat cgaatcaagc gctgtgaacg ttcccccctt ttgggtgaag
     9241 acgcatgtca gatccgcgtc actcaccgca acctgtccca tgaacatctg ccgtaccgtg
     9301 atcgcaccgc cttcttgtgt gaacgtcccg aaaccgttgc gccccacgca aacaccgaga
     9361 tttggcacag acgcatcgag tgtgccctta agcatacgca cgctccctgt gcttgacgca
     9421 ccagcgtgtc cgaggctcag gccgcctgcc cctgaaaaac gaagcacgcc atcctcaatg
     9481 atcatgtccg ccgtgccatt ccatccgagc gcaatcattt catcggaatt gaaatcgatc
     9541 accgtatctg ccctgcggat cgtggccaca ctctttgtcg gcacatttgc gttgggatta
     9601 tatcccagat aaaattcccc gcgcagatca tgctggccgc cgttgaccgt gagtcgcccg
     9661 gcatccgcgc cgccgccaaa gtggaacttt ttgaggcttg tccatgcccc accctcaatg
     9721 gtgatcttcg ggtcggtgtt tgccggccag gcgttgacca acgcattcgt ccacacgcaa
     9781 tccttcagga tcatctcccc gttcacacgg aactgcgcac tgtcattccc tcgataagaa
     9841 tcgatcaatg aattggtcgc ggcaaacaca caatcgacac cggcaatatc attgccgatc
     9901 ttggtttcct ttgagaccag ccacgaactg ttgaggacgc gcacacgtgt cggcgcattc
     9961 gccgaccatc ccaagcccaa ttcatgcccg acttccacga cgccgccatc cgttacgtca
    10021 aagtcgaaat cggccccgtt ggacactccg gaaaatttgt agtcctgact ttcagagaag
    10081 accattctgg cgttgttcgt cacaacaaag tggatatcgc gcggtttccc cgcataaccc
    10141 aaagtcatcc gggcgatatt gaatataccg ccatcagtga ccgtcacgtc gactgccgta
    10201 cgtgaatccc cccccccgcc tgatggaata ttcaggttcg cagccgcagt caacacgccg
    10261 ccatccacga caagctccgt acgtgccgca cgtgagcgca cattgatgtt ggcgaccttc
    10321 gcggtcacgc cgttgcgcac ctcgacacgg tgtacagccc ccgcgagtgt cgccgcgtca
    10381 ttcccgtgcg aaaccgtcca atcgtaattg tgtgccgtgc cggaaggatc atgaaaatta
    10441 aactcgccgg agtggaatat aatccccgcc acaccgtcct gattataggc gtggaataca
    10501 gggttcgtga acttaaacgg cgcacccctc ttgtcattat acatttcaaa cgaaaagaaa
    10561 tgactgtact gatcgtaaaa ggcgaaaggc tcaccttgat aaatcgccgt cgcgtacgtt
    10621 ggaggcgccc acaccgtatt cgagccgcag aaagtaattt ccgtcccgtc cgtgttggca
    10681 tcgacacgcc acgctacggg atttgtccaa gtgccttccg gcagtgtgac ggtatacttt
    10741 ccctgtgccg cattgtacca ccaggcttga ccattcaccg tgtccggtac cgccgcagga
    10801 ttccacttcg tagcgtcgtt aaacgcgccg ctcaccggtg ccgcccattg gtttcccaaa
    10861 gcggcaaatg caccgccgag catcaccaaa ctaaaaaatg caattctttt catcttcaac
    10921 ctcatttcac taccatccca tagggtagac cggtaaccca ccgaccccat agtcggtacg
    10981 cattatgcgg ggggggggta aaagtcaacc ctaaattttt caaaaaagtg tcggcttcgg
    11041 aggtgtctgc ctgtaaccta caggcatcat gaaaaaacga attcccaaat tgcttggggc
    11101 cgcgcctccg aaaccaacaa agatagtgta caccgtgctg cggcagttcg tccagtggtt
    11161 tcccggtaat cggttaatga caggggtgtg cgaagatcat gcgttctgat gatgaaaagg
    11221 gggagcagag agtcgggagc tcgggcgtcc tcgcccgcaa caacgtgtct tgcaaaaggc
    11281 tgacgcgccc acttccctga gggtcaaacc gcgcatcgga atcacgatcg gcctttcaca
    11341 gagcgggcgg ggacgcccgc gctcccgact gcgcacacac aaaccacgca ctaaactcgc
    11401 atgccccctg tcaccaaccg attacggatt cccgccggga tgatccagcg gctcgccgaa
    11461 gagtataagc tcgacatccg caagtggtcg gcggcgagcc atgtggtcgc gctgatgtac
    11521 ggccagctcg cgggaaaact tcaaacctga aacctcaaaa aagacaccaa aaaaccaagc
    11581 aaatccgcat gggtaagggt gaactgtgcc gcctatggga tgctattgct cacaaaacag
    11641 cgtcacgctg gattttccgc agatgatcgc gttgttggcg acccggctgg tcttctgaac
    11701 ggactgcaat cccttgttgt tggtgtgcgg cacaaaataa gagcaggagg acgaaacatc
    11761 aagccgcaac acatcgccgg gactgatcct gaaggcgtga tctgaaaaac agaaatccac
    11821 cgccttctcc gtgcccggcg tatagt
//
LOCUS       DAOSLE010000081        11742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5456_length_11742_cov_8.079661, whole genome shotgun sequence.
ACCESSION   DAOSLE010000081 DAOSLE010000000
VERSION     DAOSLE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5456_length_11742_cov_8.079661"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..2040)
                     /locus_tag="PK576_06795"
     CDS             complement(157..2040)
                     /locus_tag="PK576_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61337.1"
                     /translation="MIPLVALLAAITPLRIIPNKIVYPVNESGLMTVVLTNKASDQAT
                     STVKVEEIWGIEGASRCIHEAGISLAGHEGRTLKIPWPGSRERYGREIRVTTIVDGNP
                     TGSRSEYFNVIDEWWRVNQGCGLSTARGVVTPGLKHMLDHYGYGTNDWHDCAGQMTHV
                     AWERNMPGMGPFMGYHTMETRWQMQKSSVGGNFITTTYPDDMKWITPNGSAPRWTGKI
                     RKDTRISHDWGFHHTRFTIYFMEGPYGFELARQNPEYMLRNARGQYEGRYMDVNTDPV
                     KISVQDNPQHYPWTYVEPNFFREDAFDWAIDDLVTCVTALEEDGVYFDGRYMKKQGYD
                     AFGNNLQKVYDRDLCVARNMEKTKAKIFDANPNAYIWSNGGSAADPELVIASHPQSGL
                     LNEIQWPFLLNPSRSDHSYRGFLESLLKTRNATWFGGKYVKTPSKIMHCGYLATRWDA
                     KFILEHRESFVMARHVMSIIASVCAHPFASSVVFRPFKQMMTRYSEFFWHEDMNIMKD
                     GYKKFAADSLREIWYDDLIYIRETPNFTQYLIHLVNAPEQEFCDDTVIEEPPEADDVE
                     VSTKLFAPGGVKAWTIKPHGFLSDVYEPSCIAVTPKEVKGETVFVVPPFRYYTLLVIR
                     KYK"
     gene            complement(2037..3650)
                     /locus_tag="PK576_06800"
     CDS             complement(2037..3650)
                     /locus_tag="PK576_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61338.1"
                     /translation="MKRKTVAGMTALLAGFALASMPELEPAFSATQVEKGAAKLVWRK
                     TGEGVETTVLSEQDAGLDLAGVTLTRKTSEAPGRREVVYTVENGTKELRYASFGWRTV
                     FSIADGNDRNWFPTTDNVIDLTSQSSLWGYYTKPGNWQFALVEPWFAAYNPGHKKGFA
                     FLFDFNTLCAAFGANDMMTRGVLFDGGMLPPGKKVTVTTVVRELSGLPSLATVNDRFA
                     AGFSGPETSPSLSVLAFEDLSLDGTATQTDVNGKILGSAVVKADVKAGRVANVCVIKS
                     DKPETQSVLSADLNGIKFEFFRENGFRMASLPMVPAVWTHHRPMPVKKLEVPIAVKQT
                     AKNKALLLFGFYANFFRFPEMFPEFEFTILSATPQGIMNSPPYSTIGEYKYIFMGDVN
                     EESVRPIMTRLAAYVNSGGTLVLGGGPFAYGCGGYTGTFLEEMLPVKTRPFDCLPACV
                     SDKDGRTAVAFDTGDAKVFWIHRDEVKPGAEVLLKTVAGDPLLVKGRYGEGTVYAFLG
                     APIGDAARDAKAFWNDNPSYLETLKGLLK"
     gene            complement(3751..6867)
                     /locus_tag="PK576_06805"
     CDS             complement(3751..6867)
                     /locus_tag="PK576_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61339.1"
                     /translation="MRRIVLLAVAALCAAHGAEPWEEAVRRSAVVFDRPNGPGCVREK
                     KIHNGKVEVQAFLTGRPEYVMPVGAGNLSAMVNFGDDTFEIHLSQADWLFPEVNPVKT
                     SASDPQLMSPGHVAVKFADLSTNRITRFEQKMDMSRGRVTLSIGTTKGEIAAEIFGDR
                     ATGALVLYVNDAREGLAAPVVTTERKMESLAVVRDRAESRKFKLAIGADETAAKLSLG
                     TDDAMLAGARDSWWREYWRRGWIRLEGDEKAKNLEKWWYVNVYAYANVGYARLPPKFN
                     GGPGIVTDDIRCWGRSMWWQNTREMIWPMPAANRLEFAKRYLEFYSDCLGNLQNEIET
                     RYPEFKPFPNVIVLPEIMDILRSPLFAQEDRPLPEVTAPYVLPADAERKSSREARLAR
                     NPSHVSHIYSSGTELLQQMIEYVRFSGDRTFLPRIAKWLRANAELYLVLLEKGEDGKY
                     HTYATHVNESWWLVDDSIVDLAAARFTLAMTVAHGERFGFPKNLIAVAKDRLDHLADF
                     PHGGVYKLEKMTVAAIAPEDTVYHPCRIALGLSKANFENNELYLVFPFAMAGVDEEGP
                     RRTRALETWKHGLGAHGGFGWLPVSVAVARMRDPKAADVVYNHGHNTCGWPYGGGRSP
                     GSPMYIGAPVEDTVYLDGSCVVQTGVQELLLQSHAEEPDSRFFEGGPIRFVPCVPKSW
                     SGAFKLRARGGFIVEGTFKDGAIKKTRVTSERGNRFVWIAPATGEKKSRETKIGEIFD
                     PFARNVLPWGQDHRIGGTLLRGKDCLYAVGGGNRRGMGQIVRVELGTDGLPTGKASNV
                     GQIIEGNPGATCAAISGKWLFVAGGEIRKRTVRFEIAPDGSLTNRTEISDENMPQFSQ
                     AGMVASGTYLIVLGGWQSRQVYAAEVSSDGGLGPWIKQRPLPSISFCGNRVFRYGNRI
                     YVSGNSVFRSTTDRVYSAEVDENGLCTRWRRFAELPEEAVAYDFTLMPDGKSVFYLSM
                     DSLAMYTAPLDQGGEIGEWRKYSETFPEGPYTAMLGIPLNASHYLRYAILLDDPRQFL
                     SADIVELE"
     gene            complement(6900..9401)
                     /locus_tag="PK576_06810"
     CDS             complement(6900..9401)
                     /locus_tag="PK576_06810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE61340.1"
                     /translation="MVQDAKGTVALERSYRMLRMLSDCNRILVRSTDESRLLNDLCRL
                     LLEQGGCTAVWVGIVQDDITGTVRTAAHAGFQENIPAAFGLSERESDAAFEAWVAQVI
                     RTGQMQCCDDPFRAFAGMRRTFASQQGDVLSLLCFPLVSPSATIGAATLIVPEPGPHP
                     DEILGSLQKLACDMAFGIERLRIEGARKRATDAQRSSELRFWRLLEGMPDSVVIQTGG
                     RLTYANPTACTLLGASTPDELLGRPVLDFFKQDKHAYIKERIAQLDTRGDYAPAVEHT
                     LLRLDGAPVTVEMTTAPLRYRDQVRHEDRDGMLVFMRNITARKLQEHLYESLVEDTPA
                     HICRFRPDGELTFASGVVSAFLGLDKGEIVGANAFDFLLPEERMLARNALMNTSAEYP
                     RAEYTVSGRRADGQLRQVRCLSRAIFGPDGSASEFQMVAFDITDQLNLEERLSQTNKL
                     ETIGMLAGGIAHDFNNMLQTILGFGELALLETPPPDPRASDVQEVIAAANRAKRLTGQ
                     LLAFSRNSPMEITRVQLNRELENDQRMLARLLGENIKIVLELDETLPDILADAGHVEQ
                     VLLNLAVNARDAMPDGGTLTLQTTCVTLTDDDDRLHPGAVPGDYVCWSISDTGCGIPP
                     HIQRKIFDPFFTTKPKDRGTGLGLSTVYGIVRQLNGWILVDSEPGQGTTFAVYFPVAK
                     GVPTKQTEEHGTVSRSARRDARILVVEDQEKVAHVVERIFASRGFDTRVVGGVAEALA
                     ALEEPGSAYDLVFCDVALEDGNGIELAEKISERNPDMRFLFTSGYVDEKSGWTAIKQR
                     GWKCLVKPCPANELLAAVDEVLARDMAVPHGGSYG"
     gene            complement(9430..11649)
                     /locus_tag="PK576_06815"
     CDS             complement(9430..11649)
                     /locus_tag="PK576_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE61341.1"
                     /translation="MKQTKTMRCASGIKGSGRTIKAVCYAMACGVILSVCVLMSAAYA
                     LEKSARAGGYAGEAVVFCSARWALLVNGGIAVLGLVFLIWCLVLHRQVVKKTIALEDE
                     RRRAIDAQQDSEFHFQQLMDGMPDAVFIRTDGRLAYANPVACALLGASTPVELSDQPV
                     LGFFPQGKQAYITERIAQLDAGNGYVLAVEHTLLRLDGAPVVVEITTAPLRYKGRSST
                     LVFVRDVSARKRQEQLYESLVEDTPAIICRYKPEGELTFINSAISVFLERDKDEIVGA
                     NAFDFLLPEERVLAKNILRGTSPEDPRAEYTVINLRRADGQLRQMRCLSRAIIGPDGS
                     ISEFQTIGFDITDQRNLEERLSQTNKLETIGLLAGGIAHDFNNVLQTILGFSELALLE
                     TPLPDPRASDIQEIITAANRAKRLTGQLLAFSRNSPMKITRVQLNQEIERDQRMLARL
                     LGEDITIELVLDETLPEILADTGHIEQVLLNLAVNARDAMPDGGTLILQTACVTLAGN
                     DKRLQPEAAPGDYVCWSISDTGCGIPPHIQAKVFDPFFTTKPKDRGTGLGLSTAYGIV
                     RQLNGWILLDSEPGRGTTFSVYFPVAQEVSTEQTEESGTVPRHVRRNARILVVEDQEK
                     VAHVAERVLASHGFRTRVARGVAEAHAALAEPGAAYDLIFCDVVLRDGNGVDLAETIS
                     AQNPDMRFLFTSGYVDEKSHWKAIKQRGWKCLIKPCPASDLLAAVDEALAYDTDADG"
BASE COUNT         2455 a         3646 c         3153 g         2488 t
ORIGIN      
        1 gacgttcccg atcccgatag cgatcccgat cccgatcccg atcccgaccc cgatttggat
       61 gaaaccagaa accatgaaac catgaaacct tgaaaccatg aaacccagaa accatgaaac
      121 ccaagagagt tgaaggttca actgctcttt ttaggtttac ttgtacttcc tgattacgag
      181 aagcgtgtaa tacctgaatg gcggcaccac gaaaacggtc tcgcccttca cctccttcgg
      241 tgtcacggcg atgcatgagg gttcgtagac gtcggacaga aaaccgtgag gcttaatcgt
      301 ccaggctttg actccgccgg gggcgaaaag tttcgtcgag acttccacat catcggcttc
      361 gggcggttct tcgatgaccg tatcatcgca aaactcctgt tcgggagcat tcacgagatg
      421 aatcagatac tgggtgaaat ttggtgtttc tctgatgtag atcagatcat cgtaccaaat
      481 ttcgcgcaga gaatcggctg caaacttctt gtaaccgtct ttcatgatgt tcatatcctc
      541 gtgccagaag aactcggagt aacgcgtcat catctgcttg aaaggacgga aaacgacact
      601 gctggcaaac ggatgcgcgc agacggatgc gattatggac atcacatgcc gcgccatcac
      661 gaaactctct cggtgctcca agatgaactt cgcatcccac cgggtcgcga gatagccgca
      721 atgcatgatt ttcgatggcg tcttcacgta cttgccgccg aaccatgtcg cattacgggt
      781 tttcagcagc gactccagaa aaccgcggta cgaatgatcc gaacgcgacg gattgaggag
      841 aaacggccac tgaatttcat ttagaagccc gctctgagga tgactcgcga tcacaagttc
      901 gggatccgcc gcactgcctc cgttggacca gatgtaggcg tttggattcg cgtcgaagat
      961 cttcgccttc gtcttttcca tgttgcgcgc gacacacagg tcccggtcat agactttctg
     1021 cagattgttc ccgaaagcgt cgtatccctg tttcttcatg taacgcccgt cgaaatagac
     1081 gccatcctct tcaagtgccg tgacgcatgt cacgaggtcg tcaatcgccc agtcgaaggc
     1141 gtcttcgcgg aaaaaattcg gctcgacgta ggtccaggga tagtgctgcg gattgtcctg
     1201 cacggaaatc ttcaccggat cggtgttcac atccatatac cgcccctcgt actgtccgcg
     1261 cgcgttacgc aacatatact ctgggttctg acgcgcgagt tcgaagccgt atggaccctc
     1321 cataaagtag attgtgaaac gggtatgatg gaatccccag tcatgcgaga tgcgcgtgtc
     1381 cttccggatc tttccggtcc atcgcggggc gctaccgttc ggcgtgatcc atttcatatc
     1441 gtcgggatac gttgtcgtga tgaagtttcc gccgacagac gatttctgca tctgccagcg
     1501 tgtctccatc gtgtgatacc ccatgaacgg ccccatgccg ggcatgttgc gttcccacgc
     1561 cacatgcgtc atctgccctg cgcagtcatg ccaatcgttt gtcccgtaac cgtagtggtc
     1621 gagcatgtgc ttcaggcccg gtgtgaccac cccgcgcgcc gtactgagcc cgcacccctg
     1681 gttcacgcgc caccactcgt cgatgacatt gaaatattcc gagcgcgacc cggtcgggtt
     1741 cccatccaca atggtcgtca cgcgtatttc gcgcccgtac ctttcccggc tccccggcca
     1801 gggaattttc agcgtccttc cctcatggcc ggccaaggat attccggcct catgaatgca
     1861 gcgggaagca ccttcaatcc cccagatttc ttcaactttc acggtcgagg tggcttgatc
     1921 cgacgccttg tttgtcaaca cgacagtcat caatccggac tcgttaaccg gatagacaat
     1981 cttgttcggg ataatcctga gcggcgtgat cgccgcgagc agagcaacga gaggtatcat
     2041 ttgaggagtc ccttcaacgt ttcaagatag cttgggttgt cattccaaaa ggctttcgcg
     2101 tcgcgggccg cgtcaccaat cggcgcaccg aggaacgcgt agaccgtccc ttccccgtac
     2161 ctgcccttca caagaagcgg atcgccggca acggttttga ggaggacctc cgctccgggt
     2221 ttcacctcat cccgatggat ccagaagacc ttcgcatctc ccgtgtcgaa agcgactgcg
     2281 gtacggccat ccttgtccga gacacacgcc ggaagacagt cgaagggccg cgtcttcacg
     2341 ggcagcattt cttcgagaaa cgttcccgtg tatccaccgc agccgtacgc gaagggaccg
     2401 ccgcccagaa cgagtgtccc gccgctattg acatacgctg caagacgcgt catgatcgga
     2461 cgcacgcttt cctcattcac atcgcccatg aaaatatatt tgtactcgcc aatcgtcgaa
     2521 tacggaggac tgttcataat gccttgcggg gttgcggaaa ggatcgtaaa ttcgaattcg
     2581 ggaaacatct ccggaaaacg aaagaagttc gcatagaagc cgaacaggag gagcgccttg
     2641 ttcttcgcgg tttgtttcac tgcgatggga acttcgagtt tcttaaccgg catcggccgg
     2701 tgatgcgtcc aaacggctgg caccatcggg agagacgcca tcctgaaacc gttctcacgg
     2761 aaaaactcga actttatgcc gttcaggtct gcggagagga cggactgcgt ttcgggcttg
     2821 tcgctcttga tcacgcagac gttcgcgacc cgtccggctt tcacgtccgc cttcacaact
     2881 gcggaaccga ggatttttcc gtttacgtcc gtctgtgtgg ccgtgccgtc gagagacaga
     2941 tcctcgaaag cgaggacact cagcgacgga gaggtttccg gccccgagaa tccggcggcg
     3001 aaccggtcat tcaccgtggc aagggacggt agtcccgaga gttcgcgcac gacggttgtt
     3061 acggtgacct tcttgccggg cggcagcatg ccgccatcaa agagcacgcc ccgcgtcatc
     3121 atgtcgttcg cgccgaacgc ggcgcaaagc gtgttgaagt cgaaaagaaa tgcgaacccc
     3181 ttcttgtggc cggggttgta tgcggcgaac cacggctcga caagcgcgaa ttgccagttg
     3241 ccgggcttcg tgtaataccc ccacagggaa ctttgcgatg tgagatcgat cacgttatcc
     3301 gtggtgggga accagttccg gtcgttgccg tccgcaatcg aaaaaaccgt gcgccaaccg
     3361 aacgaagcgt agcggagttc ctttgttccg ttttcgaccg tgtacacgac ctcacggcgt
     3421 ccgggcgctt cggacgtttt ccgcgtaagc gtcacgccag cgagatcgag ccccgcgtcc
     3481 tgttccgaaa gcacggtcgt ctcgacgccc tctccggttt tcctccagac gagcttcgcc
     3541 gcacccttct cgacctgcgt ggcggaaaaa gccggttcga gttctggcat cgatgccaac
     3601 gcaaatccgg ccagcagggc ggtcatgccg gcaaccgttt ttctcttcat atcgcccctc
     3661 ctcgaaacat ggtcgctccg tgacaaccgg acgcaaaccc caacgatata ccgaaagacg
     3721 ccatagacat cccgactctc ttgttttcgt tcattccagt tcgacgatgt cggctgagag
     3781 aaactggcgc ggatcgtcca agaggatcgc ataacgcagg taatgagagg cgttaagagg
     3841 aatgccgagc attgcggtgt aggggccttc aggaaaagtt tcggagtatt ttctccactc
     3901 gccaatctcc cctccctgat cgaggggcgc tgtatacatg gcgagggagt ccatgcttag
     3961 atagaacacc gatttgccgt caggcatcag cgtgaagtcg taagcaacag cctcttcggg
     4021 cagctcggca aatcggcgcc accgggtgca aaggccgttc tcatcgactt cagcggaata
     4081 gacacggtcg gtcgtgctcc gaaagacgga attaccggaa acatagatgc ggttgccgta
     4141 gcggaacacg cggttgccgc agaaggaaat cgagggcaag gggcgctgtt tgatccaagg
     4201 gccgagtccg ccgtccgaac tcacttccgc cgcatagact tggcggctct gccagccgcc
     4261 cagaacgatc agatacgttc cgctcgccac cataccggcc tgactgaact gcggcatgtt
     4321 ctcatcagaa atctccgtcc gattcgtgag tgagccgtcc ggagcgattt caaaacggac
     4381 ggttcgtttc cggatttcgc caccggctac gaaaagccac ttgccggaaa tcgccgcaca
     4441 ggtcgcaccc ggattgccct cgataatctg gccgacgttg gacgcttttc cggtcggcag
     4501 cccgtctgtg cccagttcga cacgcacgat ctgccccatg cctctacggt tgccgccgcc
     4561 gacagcatag aggcaatctt tgccgcgcag gagcgtgccg ccgatccggt ggtcctggcc
     4621 ccacggcaac acgttacgtg cgaacgggtc gaagatctcg ccgattttcg tttctcgcga
     4681 tttcttctct cccgtcgcag gggcgatcca aacgaaacgg ttaccgcgtt cgctcgtgac
     4741 gcgcgtcttc ttgatggccc cgtccttgaa cgtgccttcc acgataaacc cgccgcgtgc
     4801 acgcaacttg aatgcgccgc tccagctttt cggcacgcat gggacaaaac gaatcggccc
     4861 tccttcgaag aaacgcgaat cgggttcttc cgcgtggctc tgcagtaaca actcctgcac
     4921 gccggtctgc accacgcagg aaccgtctag gtagacagtg tcttccaccg gagcgccgat
     4981 gtacatcgga gatcccggcg accgaccgcc gccataaggc cagccgcagg tgttgtggcc
     5041 gtgattgtag acgacgtcgg ctgccttggg atcacgcatg cgcgccactg ccacggagac
     5101 cggcaaccag ccaaacccac cgtgtgcgcc taaaccgtgt ttccacgttt cgagcgcacg
     5161 cgtgcggcgg ggtccctcct cgtccacgcc cgccatcgcg aagggaaaaa caagatatag
     5221 ttcgttgttc tcgaagttcg ccttggacaa gcccagtgcg atacggcagg gatgataaac
     5281 cgtatcctcc ggcgcgatcg ccgccacagt catcttttcg agcttgtaga caccaccgtg
     5341 cggaaaatcg gccaaatggt cgagacggtc ctttgccacc gcgatcaggt tcttagggaa
     5401 gccgaacctc tcgccatgcg cgaccgtcat cgcaagcgtg aaacgtgcgg cggcaaggtc
     5461 cacgatactg tcatccacga gccaccaaga ctcgttcacg tgtgtcgcat acgtgtggta
     5521 tttgccatcc tctccttttt cgagcagaac gagatagagt tccgcattgg cgcgaagcca
     5581 cttggcgatg cgcggcagga aggtcctatc ccctgaaaat cgcacgtact cgatcatctg
     5641 ctggaggagt tccgtgccgg aagaatagat gtgggagaca tgcgacgggt tacgcgcaag
     5701 acgtgcctcg cgggaggatt tgcgctccgc gtcagcgggg agcacgtacg gcgccgtcac
     5761 ctcaggaagc ggacgatcct cctgcgcaaa gagcggcgaa cggaggatgt ccattatttc
     5821 aggcaggaca atcacgttcg gaaacggctt gaactctgga tagcgcgttt cgatctcgtt
     5881 ttggagatta ccgagacagt ccgaatagaa ctccagataa cgcttcgcga attcgagacg
     5941 gttcgccgca ggcatcggcc agatcatctc gcgtgtgttc tgccaccaca tgctccgtcc
     6001 ccagcagcgg atgtcgtccg tgacgatgcc ggggccacca ttgaacttgg gtggcagtct
     6061 cgcgtacccg acattcgcat aggcgtatac gttcacatac caccactttt ccaggttttt
     6121 cgccttttcg tcgccttcca gccggatcca gccgcggcgc cagtactcgc gccaccacga
     6181 gtcccgcgca ccggcgagca tcgcgtcatc cgtcccgagc gaaagcttgg ccgccgtttc
     6241 gtcggcaccg atcgcgagct tgaacttcct ggactccgct ctgtcccgca caacggcaag
     6301 ggactccatt ttcctttcgg ttgtcacaac cggtgccgcg agaccctccc gcgcgtcgtt
     6361 gacgtagaga accagcgcac ccgtcgcacg gtcgccgaaa atctcggcgg cgatctcccc
     6421 cttcgttgtc ccgatcgaca gcgtcacacg tcccctgctc atatccattt tctgttcaaa
     6481 ccgcgtgata cggttcgtgg aaaggtccgc gaattttacc gcgacgtgcc cgggcgacat
     6541 gagttgcgga tcggacgccg acgttttgac agggttcacc tctggaaaaa gccagtccgc
     6601 ctgcgagaga tggatttcaa acgtgtcgtc cccgaagttc accatggcgg aaagatttcc
     6661 cgcaccgaca ggcatcacat attcgggtcg tccggtgagg aacgcttgca cctccacctt
     6721 gccgttgtgg atttttttct cccggacgca tcccggaccg ttcggtcgat caaacacgac
     6781 cgcactgcgc cgcacagcct cttcccacgg ttccgcgccg tgcgccgcgc acaaagcggc
     6841 gacggccaac aacacgatcc gcctcatacg catcctccgg atgttccgcc cccaacacgt
     6901 catccgtagc taccgccatg tggcaccgcc atatcgcggg cgagcacctc gtccacggcg
     6961 gccagcagct cgttggcggg acaaggttta acgagacact tccagccacg ctgcttgatc
     7021 gccgtccaac cagatttctc atccacatag ccgctggtaa acaggaagcg catgtcgggg
     7081 ttccgctcac tgatcttttc ggccagttca atcccattac cgtcctccag cgcaacatcg
     7141 caaaaaacca gatcatacgc agatcccggc tcctccagcg ctgcaagcgc ctccgccacg
     7201 ccacccacca cgcgcgtatc gaacccacgt gaagcaaaga tgcgttccac aacgtgcgcc
     7261 actttttcct gatcttcgac caccaggatc ctcgcgtctc tccgtgcaga gcgtgaaacc
     7321 gtcccatgct cttccgtctg cttcgttgga actcccttgg caaccggaaa gtacacggca
     7381 aaggtggtgc cttgtcccgg ctcggaatca accagaatcc acccgttcaa ctggcggaca
     7441 atcccataga cggtcgagag ccccagaccg gttccccggt ctttcggttt agtggtaaag
     7501 aacgggtcaa aaatctttct ctggatatgc ggcgggatgc cacaacccgt atcagagatc
     7561 gaccaacaga cataatctcc gggtacagct ccggggtgta gcctgtcatc atcgtccgtg
     7621 agcgtcacgc atgtggtctg caaagtgagg gtaccgccat caggcatcgc gtcacgtgca
     7681 ttgacggcca gattcaacag tacctgctcg acatgccccg catcagcgag gatgtcgggg
     7741 agcgtctcat cgagttcgag cacgatttta atgttttctc caagcagtcg cgccaacata
     7801 cgctgatcgt tttcaagctc tcgattgagt tgaaccctcg tgatttccat cggactgtta
     7861 cggctgaagg ccagcagttg tccggtcaac cgtttggcac ggttggcagc ggcgatgacc
     7921 tcttgaacgt ccgaggcgcg cggatcaggc ggcggcgttt ccaacagcgc caattcgccg
     7981 aagcccagga tcgtctggag catgttgtta aaatcgtgcg cgatgccgcc cgccagcata
     8041 ccgattgtct ccagcttgtt ggtctgtgac aaacgttcct caagattgag ctggtcggtg
     8101 atgtcaaacg cgaccatctg gaattcgctg gccgagccat cgggcccaaa gatcgcgcgg
     8161 ctcagacagc gcacctgacg cagttgtccg tccgccctgc ggccactcac cgtgtactcc
     8221 gcgcggggat actccgccga tgtgttcatc agggcattcc gcgcgagcat gcgctcttcc
     8281 ggcagcagaa aatcaaaggc attggcgccc acaatctccc ctttgtccag ccccaaaaac
     8341 gcactgacaa caccactggc gaaagtcagc tcgccatccg gcctgaaccg gcaaatatgc
     8401 gctggcgtgt cctctaccaa actttcgtaa aggtgttcct gaagttttct ggcggtgatg
     8461 ttacgcataa aaacgagcat gccgtctcga tcctcatgtc gaacttgatc ccgatatcga
     8521 agcggagccg tggtcatttc gaccgtcacc ggcgcgccat ccaaacgcag caacgtgtgc
     8581 tcgaccgccg gcgcatagtc acctctggtg tctaactgcg cgatacgctc cttgatgtat
     8641 gcatgtttgt cctgtttaaa gaaatcgagt acaggccggc ccaacagttc atcaggtgtt
     8701 gatgcgccca gcaacgtgca ggcggtcgga ttggcgtaag tgagccgccc gcctgtctgg
     8761 atgaccactg aatccggcat tccttcgagc aaccgccaga aacggagttc gctgctccgc
     8821 tgcgcatcgg tcgcgcgttt tcgtgcgcct tctatacgga ggcgttcaat gccgaacgcc
     8881 atgtcgcagg caagtttctg aagcgacccc agtatctcgt ctgggtgcgg tcctggttcc
     8941 ggcacgatga gggtcgcggc tccgatcgtg gcactgggcg agacgagcgg gaagcagagc
     9001 agcgaaagca cgtcgccttg ctgggaggcg aacgtccggc gcattccggc gaacgcgcgg
     9061 aacggatcgt cgcaacactg catctgcccc gtacggatga cctgggcaac ccaagcttca
     9121 aacgctgcgt cactttccct ctccgacagc ccgaaggcag ccggtatgtt ttcctgaaag
     9181 cctgcatgtg cggccgtcct gaccgttccc gttatgtcgt cctgcacaat gccgacccag
     9241 accgccgtac accctccctg ctccaacaag aggcggcaca gatcattgag caatctgctt
     9301 tcgtccgtgc tgcgcaccaa gatccgattg cagtcggaga gcatgcggag catacggtag
     9361 ctccgttcca gcgcgaccgt tcctttcgca tcttgcacca tcgttgttgc cttcccgttc
     9421 ataacctgat tacccatcgg catcggtatc gtatgccagc gcctcgtcca cggcagccaa
     9481 caggtcgctg gcgggacaag gcttgatgag acatttccag ccacgctgct tgatcgcctt
     9541 ccaatgggac ttctcatcca cgtagccact ggtaaacaga aagcgcatat cggggttctg
     9601 cgcgctgatc gtttcggcca gatcgacgcc gttgccgtcc ctcagcacga catcgcaaaa
     9661 gatcaggtca tacgccgccc ccggctccgc caacgctgcg tgcgcctctg cgacgccgcg
     9721 cgccacgcgc gtcctgaacc catgtgaggc gaggacgcgt tccgcaacgt gcgccacttt
     9781 ttcctgatct tcgaccacca gaatcctcgc gttcctccgc acatggcgtg gaaccgtccc
     9841 ggactcctcc gtctgctccg ttgaaacctc ctgggcgacc ggaaagtaca ccgaaaaggt
     9901 ggtgccgcgt cccggctcgg agtcgagcag aatccacccg ttcaactggc ggacgattcc
     9961 ataggccgtc gagagcccca ggccggttcc ccggtccttc ggcttggtgg tgaagaatgg
    10021 atcaaaaacc ttcgcctgga tatgcggcgg gatgccgcac cccgtgtcag aaatcgacca
    10081 gcagacgtaa tcgccgggcg cagcctcggg ttgcagccgt ttatcgttgc cggcgagcgt
    10141 cacacatgcg gtctgcaaaa tgagagtgcc gccatcgggc atcgcatcac gtgcattgac
    10201 ggccagattc aacaacacct gctcaatatg ccccgtatcg gcgagaatct cgggaagcgt
    10261 ttcatccaac acgagttcga tcgtgatgtc ttctccaagc aatcgcgcca gcatgcgctg
    10321 gtcgcgttca atctcttgat tgagttgaac cctcgtgatt ttcatcggac tgttgcggct
    10381 gaaggccaac agttgcccgg tcaaccgttt ggcacggttg gcggcggtga tgatctcttg
    10441 aatatccgag gcgcgcggat caggcagcgg cgtttctagc agggccagct cgctgaatcc
    10501 cagaatcgtc tgaagcacgt tgttaaagtc gtgcgcgata ccgcccgcca acaggccgat
    10561 cgtctccagc ttattggttt gcgacaggcg ttcctcaaga ttgcgttgat cggtgatgtc
    10621 gaacccgatc gtttggaatt cgctgatcga gccatcgggc ccaataatcg cgcgactcag
    10681 acagcgcatc tggcgcagtt gcccgtccgc cctgcgaaga tttatcaccg tgtactccgc
    10741 gcgggggtct tccggcgatg tgcccctcag gatgtttttc gcgagcacgc gctcttccgg
    10801 caacaggaaa tcaaaagcgt tggctcccac gatctcatct ttatcccgct ccaaaaacac
    10861 actgatggca ctgttgatga aagtcagctc gccttccggc ttgtaccgac aaataatcgc
    10921 cggggtgtct tccaccaagc tttcgtaaag ttgttcctga cgttttctag cggagacatc
    10981 acgcacgaaa acaagcgtgc tgctacgccc cttataccga agcggcgcag tggtgatttc
    11041 gaccacaacc ggcgcgccat ccagacgtag cagcgtgtgt tcgactgcta gcacatagcc
    11101 attcccggcg tctaactgcg cgatacgttc tgtaatgtat gcttgtttgc cctgcggaaa
    11161 aaaacctaac accggctggt ccgacagttc aacgggtgtt gatgcgccca gcaacgcaca
    11221 ggcgaccgga ttggcatagg caagtcgccc gtctgtccgg ataaataccg catccggcat
    11281 cccgtccatc agctgctgga agtggaattc gctgtcctgc tgcgcatcaa tcgcgcgtcg
    11341 ccgttcgtcc tccagtgcga tcgttttctt tactacctga cggtgcaaga caaggcacca
    11401 tatcagaaaa acgagcccca agactgcaat accgccgttt accagcagcg cccaccgcgc
    11461 cgagcagaag accacggctt caccggcgta acctccggca cgcgcagact tttcgagtgc
    11521 gtacgctgcg ctcatcagca cgcaaacaga caaaattacg ccacatgcca tcgcatagca
    11581 aacggctttt atggtccgac cgctaccctt gatgccgctg gcacatcgca tcgtttttgt
    11641 ctgcttcacg ctcatacccg tcccttttcg tgatacggcg cgagtatacc gcaggaggca
    11701 caacaaaagc aacggggaat cggttgatga cacaacctaa aa
//
LOCUS       DAOSLE010000082        11519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5580_length_11519_cov_7.479501, whole genome shotgun sequence.
ACCESSION   DAOSLE010000082 DAOSLE010000000
VERSION     DAOSLE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5580_length_11519_cov_7.479501"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /gene="gatB"
                     /locus_tag="PK576_06820"
     CDS             <1..1127
                     /gene="gatB"
                     /locus_tag="PK576_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE61342.1"
                     /translation="VTIETPEGPKRIRINHIHLEEDAAKINHYAQFSGVDFNRCGTPL
                     MEIVSEPDLASPDEAMAYLLALRQQLVYAGVSDCNLEEGNMRSDVNISIRPEGQTEFG
                     TRVEIKNMNTFKGIHAALKYEIARQLAVVRGNGTLVQETRRWDPEQGETQSMRSKENA
                     HDYRYFPEPDLVPVALTDVQIEAWREQLPEAPAARRERMMAEYGLPAYDAGVLADIRE
                     NADFFEAAVRASKPGMGKTVSNWFMTEVMRLLAETGQPVGACALTPAALAELVQLVEE
                     GVINGPTAKELLPELFHEGGMPAARVEARGLAQVSDDAALHAFIDQVFRENGKSVEDY
                     RAGKKAAAGFLVGQVMKVSHGKADPKRVGRLVAERLATRT"
     gene            1159..2853
                     /locus_tag="PK576_06825"
     CDS             1159..2853
                     /locus_tag="PK576_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE61343.1"
                     /translation="MTSNDDYVVQLLREQSYVSAEQVEAVANSIKQEGETTLDILVQT
                     GIVTEDDVLSLVAAQFGMEYVHVNPDAIDPAVTEVVPADVARKYGVVPVTQTEDTVTV
                     ALSDPMAYDTVDSLRYVLKMNVEAVVAPRSEVRAAVDRLYPESFESIYGAMSDSEIDV
                     AEKAKSDTGQESDDSDAPVIRLVSMIITEACKMRASDIHLEPMERTFRVRYRIDGVLR
                     QMDTPPKRLQAAILSRVKIMSNMKISEKRVPQDGRIQIKVQGRDLDLRVSSVPTNHGE
                     SIVMRILDKSNLSLGLPQLGFLSDDQNKFERLISLPDGVLLVTGPTGSGKTTTLYACL
                     GQINLPDRKIITVEDPVEYQMSGINQVQVNKDVGLDFSAALRSILRQAPNIVMIGEIR
                     DHETADIAMEAALTGHLVFSTLHTNDAPSAVTRLLDIGVKPFLVASALRAAMAQRLVR
                     AVCEKCRAEYKPTERELKMLGAMAKNVTVSQMYKGTGCSACGQTGYKGRKGIFEIFQI
                     DDTIQRLIFDHSPATVLRTRAREMGMRTLREDGLLKVASGMTTLEEVLRATMGDAD"
     gene            2853..3941
                     /locus_tag="PK576_06830"
     CDS             2853..3941
                     /locus_tag="PK576_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOE61344.1"
                     /translation="MSTEINMTELLQATVDEGASDLHIRVGIPPMLRIHGALTPLDSP
                     PLTPQDTESLARMITAEDKLNEIQECGGADFALPFGDLARFRVSVFKERGHFGIVLRQ
                     IPSKLLTLEQIGLPEQVKELLFRPRGLILVTGPTGSGKTTTLASMIDIINRERDVHII
                     TVEDPIEYYHPHKKSVVTQREIGHDVTSFAEAIRRALRQDPDVILVGEMRDLETMQAA
                     ITAAETGHLVFATLHTTGAAATVDRIVDAFPTDQQEQVRTQMSVGLVAVISQLLLARV
                     DKPGRVAAFEIMISTPSIRSRIRDNKTFQISSDIQTGAKFGMISLDSHLMDLYLGGLI
                     SYDDLVTKSQDPDTLVQKLKDELQSRRN"
     gene            3941..5659
                     /locus_tag="PK576_06835"
     CDS             3941..5659
                     /locus_tag="PK576_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE61345.1"
                     /translation="MAVTSTSVAPPYHPLIDLLVSNGIIDQQQAEELRDEQKNSGKPF
                     RTLLVDAGYVSEDDLLGMMAAYQGCDVIDLEMMSLDTEVIQSIPASVARMYNVLPVSA
                     TAGTITLATSDLVDPHVTDELMFVLTRDVQFVMARENDVRTRVAEYYGDESATVADML
                     NSLESDLKLDTSLDVDDTDDKSLESAASAAPVIRFVNLVLYQAVTDRASDIHFEPFET
                     DFKIRYRVDGALYEMSPPPRRLALPIISRIKVMSGLNIAERRVPQDGRIALTVAGRSV
                     DLRVSCLPTAHGESVVLRVLDRSVVSLDLENVGLPEDVYEELTLDIEKPNGIIIVTGP
                     TGSGKTTTLYSCLRRINTIDQKLLTAEEPVEYDMDGIVQVQINPSAGNTFPRVLRAFL
                     RQDPDIMMIGEIRDLETAEIAVQASLTGHLVFSTLHTNDAAGAVTRLIDMNVAPYLIA
                     STLEAVLGQRLVRTICLNCKEPYTPDDETLSRMDLKRAAVGDRPFYYGRGCSKCNGTG
                     YKGRKGVFEYLRVSDPIRELINERRPTLFIRERARELGMRTMREDAIRNVLDGYTTVD
                     EVLRYT"
     gene            5713..6618
                     /gene="trxB"
                     /locus_tag="PK576_06840"
     CDS             5713..6618
                     /gene="trxB"
                     /locus_tag="PK576_06840"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOE61346.1"
                     /translation="MVIIGSGPAGLTAAIYAARANLNPVLIEGMQPGGQLTQTTDVEN
                     FPGFENPIDGTTLVMTMRKQAERLGVRCLADEVSACALAAKEKTVTLMLGGELQTRAV
                     ILATGASARYLGLESEQRLIGKGVSACATCDGSFFKGQPVAVIGGGDTAMEDALYLSR
                     IAERVTVVHRRDTFRASKIMAGRVLASPQIEVAWNSVVDEVLGAEEVTGLRLRHVHTG
                     ALTEIPVNGVFVAIGHTPNTALFAGQVELDPEGYVVTRNTRTSLPGVFAAGDVQDRHY
                     KQAVTAAGSGCMAALEAERFLASGE"
     gene            6622..8601
                     /locus_tag="PK576_06845"
     CDS             6622..8601
                     /locus_tag="PK576_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61347.1"
                     /translation="MVESFDFDKEYSFAEAYGRLYRYARKYRFRLLAGLLAGSVTAGS
                     MLPIYQVVQPVIRNMYSVEEIVPAAHAAHTDDAVKPPSAPQRMDTNLKSLVANRANPR
                     AAATGKLPAWFDRMEAVGNRFGLSFRDEEGRMTGELLLIALVVVPLVMLVKMTALYLN
                     HYFLRWTGAKVVQDFRIELFSHLQKQSLGFFGKVDVGQLISRCTSDPHQVDNVIAHTL
                     ADLCRAPFEILFSIGFVIYFAIRNNMIETLVLVLVGFPLVLFPMAVLGSWVRRWSRRS
                     LLRISYVVSKIHENLTCIRVVKAYHTEAYEIEKYRKANHFYMKSVLRAVRIELLITPA
                     VECVAIFLCFAFITYCYFRRLTIDQIAPLLVPILVAYRPMKQLGKVQAQLERGRAALA
                     RMYSLLDLDMSLPMAARPVRKEQFEDAVRFDHVGFRYGDGPDLTIRDLSFVLPRGSLV
                     AVVGGTGSGKTTVANLLARFYDPVSGSVTMDGVDLRQIEIGDLRKLIGVVTQETVLFN
                     DTIENNIRYGMPGTTREQVVAAAQMANAHTFITDHPEGYDRVVGEKGFVLSGGERQRV
                     AIARAILKNPPILILDEATSALDTVTERLVQDAINRLMANRTTFAIAHRLSTIRNADQ
                     ILVMEKGAVIERGTHEELWAAGNKYRALCDMQMME"
     gene            8645..10258
                     /locus_tag="PK576_06850"
     CDS             8645..10258
                     /locus_tag="PK576_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOE61348.1"
                     /translation="MGGQKASLEKQIVKAGFSILVAHVLFKLAGLIQSVVMGRYLPPG
                     VFDVVYVFAFERCVYMLFLIGEEVLGPSLLPVFMRELDTKSEHDAWQFANTILTLQFF
                     VLAVVAALLCVAPQAVVAFLTQWGPTESPEKFELAARSVRTLAPALVGLSLGSTTYVL
                     LNGYKRFFLAAFGDAVWKFCVVGFLIVGTLFSREGAQMLMWGLVAGSVCKLVVHLFGL
                     HDKLRYIRPRLAWSHPAIRTFCLLALPLLAGILIAKVRDQVNDVYVLSALDESGLMQA
                     NSMGRKLQSTLLYLVPYTLSIAVFPFFCELVDKQDQVGLGQLVTRFGRMLLSVFVPFA
                     IFVAVAAVPITSLIFRGGYFDATAVQRTSVSLSFYTFVLPAAAIEMLVMQAFFANRRM
                     LSVMWIGVVFSILSMGISYIGLQVADGRELVVLAFIAGGFTLARTLKSLTLVMMLRAN
                     ARVFPFRETAVFLSRVTVAAGLAALLGWLTLHAQPLAELAGRVGDLVRLTAAGAVFGC
                     VYLGGTYVLKISEIRELCGLAIQKLSKHA"
     gene            10353..10670
                     /locus_tag="PK576_06855"
     CDS             10353..10670
                     /locus_tag="PK576_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOE61349.1"
                     /translation="MGMLDQVKQAMQMRKEAKRIQSEIEKITCEYANGGITCVARGDF
                     TIVSLKVTQEALADVIAGKPERFETMLGNVVNGALKGVKKQTQDAMTKMMQGTGMGNM
                     FGG"
     gene            10678..11298
                     /gene="recR"
                     /locus_tag="PK576_06860"
     CDS             10678..11298
                     /gene="recR"
                     /locus_tag="PK576_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020151491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOE61350.1"
                     /translation="MVPPVDNLIRALSKLPGLGRRSAERAALALLRKPELLLDTLMRA
                     LHEARETVCCCSLCGGFTAKTCEPCALCTDPMRDDSLLCVVEEPADIVSIERSGGFRG
                     RYHALMGKLSPSKRTGPSDLRLVALERRVSKGPVREVLLALSTDMEGDATAGYIGEML
                     KPSGVRITRLAFGLPADSGVGYSDPLTLKRAISGRVVCEPQEVTEP"
     gene            complement(11285..11428)
                     /locus_tag="PK576_06865"
     CDS             complement(11285..11428)
                     /locus_tag="PK576_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61351.1"
                     /translation="MGFAGPELGSAKDWTVEGLKRDYRTYVDESGKRVLPGSPCGTVI
                     TVR"
BASE COUNT         2184 a         3184 c         3782 g         2369 t
ORIGIN      
        1 gggtgaccat cgagacgccg gagggaccga aacggatccg gatcaaccat atccaccttg
       61 aagaggacgc cgcgaagatc aaccactacg cgcaattcag cggggttgat ttcaaccgtt
      121 gcggcacgcc gttgatggaa atcgtttcgg agcccgacct cgcgtcgccg gatgaggcga
      181 tggcctatct cctggcgctc cggcaacagc tcgtgtatgc gggggtgagc gactgcaatc
      241 tcgaagaggg gaatatgcgg tcggatgtga acatcagcat acggcccgaa gggcagacag
      301 aatttggcac gagggtggaa atcaaaaaca tgaacacctt caaagggatc cacgcggcgc
      361 tgaaatatga gatcgcgcgg cagctcgccg tggtgcgggg gaacggcacg ctggtgcagg
      421 agacgcggcg gtgggatccc gaacaggggg agacccaatc gatgcgcagc aaagagaatg
      481 cgcatgacta ccgttatttc cctgagccgg atctggttcc ggtggcactg acggatgtgc
      541 agatcgaggc ttggcgggag cagttgccgg aagcgccggc tgcgcgacgc gagcggatga
      601 tggcagagta cggcctcccg gcatacgatg cgggggttct ggcggatatc cgggagaacg
      661 ccgatttctt tgaggctgcg gtacgggcca gtaagcccgg tatggggaag acggtatcca
      721 actggtttat gaccgaggtc atgcggttgc tggcggaaac ggggcagccg gtgggcgcgt
      781 gcgcgctgac gcctgccgcg cttgcggaat tggtgcagct ggtggaggag ggcgtgatca
      841 acgggccgac cgccaaggag ctgctgcccg agctgtttca tgaaggcggc atgcccgccg
      901 cgcgggtcga ggcgcgcggt cttgcgcagg tgagcgatga cgccgcgctc catgcgttca
      961 tcgatcaggt gttccgggag aacggcaagt cagttgaaga ttatcgggcc ggcaaaaaag
     1021 ccgcagcggg ttttctggtc ggacaggtga tgaaagtaag ccacgggaag gcggacccca
     1081 aacgggtcgg ccgactggtg gcagagcggt tggcgacacg gacgtgagcg gaccggtcgt
     1141 gttacgggga gagcgattgt gacttcgaat gatgattatg tcgtgcaact tctgcgtgag
     1201 cagagttatg tgtccgccga gcaggtcgag gcggtcgcga actcgatcaa gcaggaaggg
     1261 gagacaacgc tcgatattct ggtccagacc gggatcgtga ccgaagatga cgtgttgagt
     1321 ctggtcgccg cgcaattcgg catggagtac gtgcatgtca accccgatgc catcgaccct
     1381 gccgtcaccg aggtggttcc ggcggatgtc gcgcgcaagt acggcgtggt tccggtcacc
     1441 cagaccgaag acacggtgac cgtggcgctg agcgacccca tggcctacga cacggtggac
     1501 agtctgcggt atgtgcttaa aatgaacgtc gaggcggttg tggcgccgcg ttccgaggtg
     1561 cgggccgcgg tcgaccggtt gtatccggag tcgtttgaga gcatttacgg ggccatgtcg
     1621 gacagcgaga tcgatgtggc ggagaaggcg aaaagcgaca cggggcagga atccgacgac
     1681 tccgatgccc cggtgatccg tctggtcagc atgatcatca cggaagcctg caagatgcgc
     1741 gcgtccgata tccatctgga accgatggaa cggacgttcc gtgtgcggta ccggatcgac
     1801 ggtgtgttgc ggcagatgga cactcccccc aagcggttgc aggcggcgat cctcagccgc
     1861 gtgaagatca tgtcgaacat gaagatctcc gagaagcggg tgccgcagga cggccggatt
     1921 cagatcaaag tgcaggggcg cgacctcgat ctgcgcgtgt cctccgtgcc gaccaaccac
     1981 ggcgagagca tcgtcatgcg tattctggac aaatccaacc tctcgctcgg tctgccgcag
     2041 ctcggcttcc tgtcggatga ccagaacaag ttcgaacgtc tgatctccct gccggatggt
     2101 gtgcttctgg tgacggggcc caccgggtcg ggaaaaacaa cgacccttta cgcctgcctc
     2161 ggccagatca atctgccgga ccggaaaatc atcacggttg aagacccggt cgaatatcag
     2221 atgtccggca tcaatcaggt gcaggtgaac aaggatgtgg ggttggattt ttccgccgcg
     2281 ctccgttcga tcctgcgtca ggcgccgaac atcgtgatga tcggtgagat ccgcgatcac
     2341 gagaccgcgg acatcgcgat ggaggcggcc ctcacgggac accttgtttt cagcacgctg
     2401 cacaccaatg acgcgcccag cgcggtcacg cgtctgctgg atatcggcgt gaagccgttt
     2461 ctggtggcgt ccgcgttgcg cgccgccatg gcgcagcggt tggtgcgcgc ggtctgcgaa
     2521 aaatgccgcg cggaatacaa gcccaccgaa cgcgaactca agatgctcgg tgccatggcc
     2581 aaaaacgtca cggtgtcgca gatgtacaag ggcaccggct gttcggcctg cggacaaacg
     2641 gggtataagg ggcgcaaggg catttttgag attttccaga tcgatgacac aatccaacgc
     2701 ctgatcttcg atcattcgcc ggccacggtg ttgcgtaccc gtgcgcggga gatgggcatg
     2761 cggacattgc gcgaggacgg cctgctcaag gtggcctcgg gcatgacgac gctggaggag
     2821 gtcttgcggg caaccatggg agacgctgac tgatgagcac agaaatcaac atgaccgaat
     2881 tattgcaagc cacggtggat gagggggcct ccgatctgca catccgcgtc gggatcccgc
     2941 cgatgctgcg cattcatggc gcgctgacgc cgttggacag tccgccgttg actcctcagg
     3001 atacggaatc gctggcccgg atgatcaccg ccgaagacaa gttgaacgag attcaagagt
     3061 gcggcggcgc ggacttcgct ttgccgttcg gtgacctggc tcgttttcgt gtctcggttt
     3121 tcaaagagcg ggggcatttc ggcatcgtgt tgcgccagat cccgagtaaa ctgctcacgc
     3181 tggagcagat cgggttgccg gagcaggtca aagagttgct tttccggccg cgcgggctga
     3241 ttctggtgac cggccccacc ggttccggaa aaaccaccac gctggccagc atgatcgaca
     3301 tcatcaaccg cgagcgcgac gtccacatca tcacggtgga ggatcccatc gaatattacc
     3361 acccgcataa aaaatcggtg gtgacccagc gcgagatcgg gcatgatgtg acgtcattcg
     3421 ccgaagcgat ccgccgtgcg ctgcgccagg atccggatgt gatcctcgtg ggggaaatgc
     3481 gcgatctgga aaccatgcag gccgcgatca cggcggcgga aaccggccat ctggtgttcg
     3541 ccacgctgca taccaccggt gcggccgcca cggtggaccg catcgtggac gccttcccca
     3601 ccgatcagca ggaacaggtc cgtacgcaga tgtccgtggg gctcgtggct gtgatttcgc
     3661 agttgcttct ggcgcgtgtg gacaagcccg gccgtgtggc ggcgttcgaa atcatgattt
     3721 cgaccccgtc catccgttcc cggattcgcg ataacaagac attccaaatc tcgtccgata
     3781 tccagaccgg agccaaattc ggcatgatct cgctcgactc gcatctgatg gacctctatc
     3841 tgggcgggct gatctcatac gatgatctcg tcaccaaatc gcaggatccg gatacgctgg
     3901 tccagaaact gaaagacgaa cttcagtcaa ggaggaactg atggctgtga cttcgacatc
     3961 cgttgcgccg ccctatcatc cgttgatcga cctgctggtc agcaacggca tcattgacca
     4021 gcagcaggcg gaagagttgc gcgacgagca gaaaaactcg ggcaagccct ttcgcaccct
     4081 gctggttgac gccggatacg tgagcgaaga cgatctgctg ggtatgatgg ccgcatatca
     4141 gggttgtgac gtgatagacc tcgagatgat gtcgctcgat acggaagtga tccagagtat
     4201 ccctgccagt gtggcccgca tgtacaatgt cctgccggtg tctgccactg ccggaaccat
     4261 cacgctggcc acaagcgatc tggttgatcc gcacgttacg gacgaattga tgttcgtgct
     4321 gacccgcgac gtgcagttcg tcatggcgcg cgaaaatgat gtgcgcacgc gtgtcgccga
     4381 atactacggc gacgagagcg cgactgtcgc ggacatgctc aacagcctcg agtcggatct
     4441 gaagctggac acctcgctgg atgtggatga tacggatgac aagagtctgg aatccgccgc
     4501 gtccgccgcg ccggtcatcc ggttcgtgaa cctcgtgctc tatcaggcgg tcacggaccg
     4561 ggcctccgac atccatttcg aaccgtttga aaccgatttc aagatccggt accgcgtgga
     4621 tggcgccctg tatgagatgt cgccgccgcc ccgccgtctg gctctgccga tcatctcgcg
     4681 cattaaggtc atgtccggcc tgaacatcgc ggaacggcgt gtgccgcagg acgggcgcat
     4741 cgcgctgacc gtggccggcc gttcggttga tctgcgtgtc tcctgcctgc cgaccgcgca
     4801 tggggaaagc gttgtgctcc gcgtactgga ccgcagcgtg gtttcgcttg atctcgagaa
     4861 cgtcggtctg ccggaagatg tctatgagga actgacgctg gacatcgaga aacccaacgg
     4921 gatcatcatc gtcacggggc cgaccggttc cgggaaaacc acgacgctgt actcctgtct
     4981 gcgccggatc aacacgattg atcagaagct gctgaccgcc gaagaaccgg tcgaatacga
     5041 catggacggc attgttcagg ttcagatcaa tccgagtgcg gggaacacct ttccaagggt
     5101 gttgcgcgcc tttctgcgtc aagaccccga catcatgatg atcggcgaaa tccgcgacct
     5161 cgagaccgcc gagatcgcgg tgcaggcctc cctcaccggc cacctggtgt tcagcacgct
     5221 gcacaccaat gatgcggccg gtgcggtcac gcgcctgatt gacatgaatg tggcgccgta
     5281 cctcatcgct tccaccttgg aggcggtgct tgggcagcgt ctggtccgca ccatctgcct
     5341 gaactgcaag gagccctaca cgccggatga tgagaccttg agccgtatgg acctgaaacg
     5401 cgccgcggtc ggggatcggc ctttttatta cggccgggga tgttccaagt gcaacggaac
     5461 cggatacaag gggcggaaag gggtgttcga atatctgcgg gtgagcgatc ccatccgcga
     5521 gttgatcaac gaacgtaggc cgaccctgtt catccgggaa cgcgcccggg agttgggtat
     5581 gcggaccatg cgggaagacg cgatccgaaa tgtgctcgac gggtatacca cggtagatga
     5641 ggtgttgcgg tacacgtagg cgggagcgcc gcttgcggtt gattccggtg aggggaggga
     5701 cttgtggaaa atgtggtgat cattgggagt ggacccgccg gtctgacggc ggcgatttat
     5761 gcggcgcgcg ccaacctgaa tccggtgctg atcgaaggca tgcagcccgg cggacagttg
     5821 acccagacga ccgacgtcga gaatttcccg ggattcgaga atccgatcga tggaaccacg
     5881 ctggtcatga ccatgcgcaa gcaggcggaa cgcctcggcg tacgctgttt ggccgacgaa
     5941 gtgtcggcat gtgcgctggc tgcgaaagag aagaccgtca cgctgatgct ggggggggaa
     6001 ctgcaaacgc gtgccgtgat ccttgccacc ggtgccagcg cgcggtattt ggggctcgag
     6061 tccgagcaac ggctgattgg caaaggcgtc tcggcctgcg ccacctgcga cgggtcattt
     6121 ttcaagggac agccggtggc ggtcatcggc gggggggata cggcgatgga ggacgcgctg
     6181 tatctctcac gcatagccga acgggtgaca gtcgtccatc gccgcgacac gttccgcgcc
     6241 tcgaaaatca tggcgggacg ggtcttggct tcgccgcaga tcgaggtggc ctggaattcg
     6301 gtggtggatg aggtgttggg tgcggaggag gtcacgggac tgcgtctgcg gcacgttcac
     6361 accggagccc tcacggagat tccggtgaac ggagtgtttg tcgcgatcgg ccatacgccc
     6421 aacactgcgc tcttcgccgg gcaggttgaa ctggatccgg agggatatgt ggtcacgcgt
     6481 aacacgcgca cctccctgcc gggcgtgttc gcggcagggg atgtgcagga ccgtcactac
     6541 aaacaggcgg tcactgccgc gggatcgggg tgtatggccg cattggaagc ggagcggttt
     6601 ttggcttcag gcgaatagga tatggttgaa tcatttgact ttgataaaga gtattcgttt
     6661 gccgaagcct acggaaggct ttaccgttat gcgcggaaat atcgcttccg cctgctggcc
     6721 ggtctgctgg ccggatcggt cacggcgggt tccatgctgc ctatttatca ggtggtgcag
     6781 ccggtcattc ggaacatgta ttcggttgag gagatcgtgc ccgccgcgca tgccgcgcac
     6841 acggatgatg cggtaaagcc gccttcggca ccgcaacgga tggacaccaa cctgaagagt
     6901 ctggtcgcca accgcgcgaa tccccgcgcg gcggctacgg gaaaactgcc ggcctggttc
     6961 gaccggatgg aggccgtggg taaccgcttc ggactctcct tccgggatga ggagggccgg
     7021 atgacgggcg aactgctcct gatcgccttg gtcgtggtgc cgcttgtcat gctggtcaaa
     7081 atgacggcgc tctatctgaa ccactacttc ttgcgctgga ccggcgcgaa agtggtgcag
     7141 gatttccgca tcgaactctt tagccacctg cagaaacagt cgctcggttt tttcgggaag
     7201 gtcgatgtcg gccaattgat aagccgatgc accagtgacc cgcaccaagt tgacaatgtg
     7261 atcgcgcaca cactggccga tttgtgccgt gccccttttg aaattctgtt ttccataggg
     7321 tttgtgatct atttcgcgat tcggaacaac atgatcgaaa cgctggtgct ggtgctcgtc
     7381 ggcttcccgt tggtgctgtt ccccatggcg gttctggggt cttgggtccg ccgctggtcc
     7441 cggaggtcgc tgctccggat ctcttatgtg gtgtccaaga tccatgagaa cctgacctgc
     7501 atccgcgtgg tcaaggcgta tcatacggag gcgtatgaga ttgagaagta ccgcaaggcg
     7561 aaccacttct atatgaagag tgtgttgcgg gccgtgcgga tcgagttgct gatcactccg
     7621 gcggtggagt gcgtagcgat tttcctctgt ttcgctttta tcacctactg ctatttccgg
     7681 cgactgacga tcgatcagat cgcgccgctg ctggtgccga ttctggtggc ctaccgtccg
     7741 atgaagcaac tcggcaaggt tcaggcccag ctggagcgcg gccgcgctgc cctggcgcgg
     7801 atgtactccc tgcttgattt ggatatgagt ctgccgatgg cggcccggcc ggtccgcaaa
     7861 gaacagttcg aggatgccgt ccgctttgac cacgtcgggt tccggtatgg ggacggacct
     7921 gacctcacga tccgcgacct ctcgttcgtg ttgccgcgtg gttcgctggt ggcggtggtt
     7981 ggagggaccg gctccggcaa gacgaccgtg gccaatctgc tggcgcggtt ttacgatccg
     8041 gtttccgggt ccgtgaccat ggacggggtg gatctgcggc agatcgagat tggggatctg
     8101 cgtaagctga tcggcgtggt gacgcaggag acggttttgt tcaacgacac cattgagaac
     8161 aatatccggt acggcatgcc gggaacgacc cgtgaacagg tggttgccgc agcccaaatg
     8221 gccaacgcgc acacgttcat cacagaccat ccggaagggt atgaccgtgt ggtgggagag
     8281 aaggggttcg tgctgagcgg cggggaacgc cagcgtgtgg cgatcgcccg cgctattctc
     8341 aaaaaccccc ccatcctgat tctggacgag gcgaccagcg cattggacac ggtgaccgaa
     8401 cggttggtgc aggatgcgat caaccggctc atggccaacc ggaccacatt cgccatcgcc
     8461 caccgtttga gtaccattcg gaatgcggat caaattctgg tgatggaaaa gggggccgtc
     8521 atcgaacgcg gtacgcacga ggagttgtgg gccgcgggca acaagtaccg cgcattgtgc
     8581 gacatgcaga tgatggagtg aggggtatcg gaggggccat cgcccgtttc cgtttggggg
     8641 acgcatgggg ggacaaaagg cgagtctcga gaaacagatc gtcaaggcgg gtttttccat
     8701 tctggtggcc cacgttttgt ttaagctggc ggggctgatc cagtccgttg tcatgggccg
     8761 ttatcttccg cccggtgtgt tcgatgtggt gtacgtgttc gccttcgagc ggtgtgtcta
     8821 tatgcttttc ctcatcggcg aggaagtgct ggggccgtcg ctgttgccgg ttttcatgcg
     8881 cgagttggac acgaagtcgg aacacgacgc atggcagttc gccaacacga tcctgaccct
     8941 gcagttcttc gtgctggcgg tcgtcgcggc gttgctgtgc gtcgcgcccc aagcggtggt
     9001 cgcgtttctc acgcagtggg gaccgaccga atcgccggag aagttcgagt tggccgcgcg
     9061 cagcgtcagg accctggctc cggctctggt ggggctttca ttggggtcga ccacctacgt
     9121 gctgctgaac ggatacaagc ggttctttct ggccgcgttc ggcgatgcgg tgtggaagtt
     9181 ctgcgttgtc gggttcctca tagtcgggac gctgttttcg cgcgagggag cccagatgct
     9241 gatgtggggc cttgtggccg gttcggtttg caagttggtt gtgcatctgt tcgggcttca
     9301 cgacaagctg cgatatatcc ggccaaggct ggcatggtcc catccggcga tccggacctt
     9361 ctgtttgctg gcgcttccgc tgctggccgg catcctgatc gccaaagtcc gcgatcaggt
     9421 caacgatgtc tatgtgttga gcgcgctgga tgaatcgggc ctcatgcagg cgaactcgat
     9481 ggggcgcaag ctgcagagca cgttgctcta tctcgtgccg tacacgctgt cgatcgcggt
     9541 tttcccgttt ttctgcgaac tggtggataa gcaggatcag gtggggttgg gacagttggt
     9601 cacgcgcttt ggccggatgt tgctctccgt gtttgtgccc ttcgcgatct ttgtggctgt
     9661 ggcagcggtg ccgatcacct cgctgatttt ccggggcggg tattttgacg cgacagcagt
     9721 tcagcggacg tcggtttcgc tttccttcta cacgttcgtc ctgccggccg cggcgatcga
     9781 gatgctggtg atgcaggcgt ttttcgcaaa ccgcaggatg ctttccgtga tgtggattgg
     9841 agtggtgttc tccatcctca gcatggggat cagttatatc ggtctccaag tcgcggacgg
     9901 acgcgagttg gtcgtgctgg ccttcatcgc aggcggcttc acgctggccc gcacactgaa
     9961 gagtctgacg ctggtcatga tgctgcgggc gaatgcccgg gtctttccgt ttcgcgagac
    10021 cgcggttttc ctttcgcgtg tcaccgtggc ggccggtctc gccgcccttt tgggatggtt
    10081 gacgctccat gcccagccgc ttgcggaact ggcggggcgc gtgggggatc tggtgcgcct
    10141 gactgcggcg ggagccgttt tcggttgcgt ctacctcgga ggcacctacg ttctgaaaat
    10201 cagtgagatc cgcgaactct gcggtttggc gatccagaaa ctcagcaaac acgcttgaga
    10261 attcagagcc ggatgtatta cactctccct gtcttgtctc ccgtaccggg gtgtgggtga
    10321 acggaatgga atcttgtaag gagtgatgga acatgggtat gttggatcag gtgaaacagg
    10381 cgatgcagat gcggaaagaa gccaagcgga ttcagtccga gatcgagaag atcacttgcg
    10441 agtacgcgaa cgggggaatc acctgtgtgg cgcgcggcga tttcacgatt gtttccctca
    10501 aggtgacgca agaggcgctc gccgatgtga tcgcgggcaa gccggagcgt tttgagacca
    10561 tgctgggcaa tgttgtcaac ggcgcgctca aaggcgtgaa gaaacagaca caggacgcca
    10621 tgaccaaaat gatgcagggc acgggcatgg gcaacatgtt cggagggtaa gccagcgatg
    10681 gtgccgccgg tcgataatct gatccgggcc ctgtccaaac tgcctgggct gggcagaagg
    10741 tcggctgaac gcgcggctct ggccttgttg cgcaaaccgg agctgttgct ggacactctg
    10801 atgcgtgccc tgcatgaggc gcgggagacg gtctgctgtt gttcgctatg cggcggtttc
    10861 actgccaaaa cgtgtgaacc gtgcgcgttg tgcacggatc cgatgcgcga tgacagcctg
    10921 ctttgcgtgg tcgaggaacc ggcggacatc gtttccatcg aacgttccgg cggtttccgc
    10981 ggccggtacc acgccctgat gggcaagctt tccccatcca aacgtaccgg tccgtcggac
    11041 ttgcggctgg tcgcgctcga gcggcgcgtc tccaaagggc cggtccggga ggtgctgctg
    11101 gcactcagca ccgacatgga gggtgacgcg acagccgggt acatcggcga gatgttgaaa
    11161 ccttccggtg tgcggatcac gcgtttggcc ttcggtctgc ccgcggacag cggtgtcggc
    11221 tattccgatc ccctgaccct caaacgagcg atctcgggtc gcgtggtctg cgagcctcag
    11281 gaagttaccg aaccgtgatc accgtgccgc acggagaacc cggcagcacc cgttttccgc
    11341 tttcatccac ataggtgcgg taatcgcgct tcaggccttc cacggtccag tcttttgcgg
    11401 atcccaactc cggtcccgcg aaccccatcc agccctgaac ctaaaaagag cagttgaacc
    11461 ttcaactctc tttggtttca aggtttcagg gtttctggtt tcagggtttc agggtttca
//
LOCUS       DAOSLE010000083        11293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5698_length_11293_cov_8.306015, whole genome shotgun sequence.
ACCESSION   DAOSLE010000083 DAOSLE010000000
VERSION     DAOSLE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5698_length_11293_cov_8.306015"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..288
                     /gene="rpmE"
                     /locus_tag="PK576_06870"
     CDS             82..288
                     /gene="rpmE"
                     /locus_tag="PK576_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOE61352.1"
                     /translation="MKKDTHPNYDDATITCACGNVIKTRSTRKEMNVNTCSACHPYFT
                     GQQTMLDTEGRVDQFMKRYRIKKA"
     gene            367..1461
                     /gene="prfA"
                     /locus_tag="PK576_06875"
     CDS             367..1461
                     /gene="prfA"
                     /locus_tag="PK576_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOE61353.1"
                     /translation="MLDKAHILQALGRVQAAEAALSDPAVLGNPRVYREKLREHAALK
                     RLESAANAYFKLLDELEGNRALESEPGGDPELAELARSEIARISERVPEAEREVLACL
                     LPSDPRDARNAVFEIRAGTGGEEAALFAAVLFRMYSKFCDAKAWKIAVLDAHATELGG
                     YKEIVFTVSGEGAYGAFKFESGGHRVQRVPETEAQGRIHTSAATVIVFPEADEADEIP
                     LPESELRVDIFCAGGHGGQGVNTTYSAIRITHLPTGLVAQCQDERSQHRNKEKALAVL
                     KARILDQRRQAEEAALGQTRLGLRGSGDRSQRIRTYNFPQNRMTDHRVNLTLYSLDRI
                     VEGELGPLLDALHAYDLDQRLAQESGLRPE"
     gene            complement(1471..2139)
                     /locus_tag="PK576_06880"
     CDS             complement(1471..2139)
                     /locus_tag="PK576_06880"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOE61354.1"
                     /translation="MTRRFLRGGPCRIARAEADVTETVFVELCAPLAGHLWLQALLVT
                     AGTCFLEDAARCGVSLLVATGHLGWGFAFACMTAGGMSGDIGLYLIGRYATSLLFRLR
                     WVSPSRLEWMEDYFRHHAIKTVVVSRFLPGARTVSNAAAGAVRYPFPRFLLLVFLSTV
                     VQSLLFLQLGVFIGETILPYLRDPRLRIAVFFLVLLMLLAAHRAFVRRRRLAPPDAEG
                     AAGK"
     gene            2425..3870
                     /locus_tag="PK576_06885"
     CDS             2425..3870
                     /locus_tag="PK576_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61355.1"
                     /translation="MNLTSKRMMMAAACAGLCAFASMTFAGDPTKWWVSDSFEVADGG
                     AADLPISQYKQTVTEIVGGGGATTTNFLWVADGLDASKIIATNSYIGQPFITNSGLRE
                     LVLELATEGSTLTRHVIGDNGVSTGKAFSASAPVYVDTMIQFRPSEDTPTIGDDVKIA
                     VYVNVNSNLVVYHGMDGADPTNSVFETEFINPDTWYRLTIEMTGYDEWTPACKVYLNK
                     IQLSHANALEGGLFLTAGPGGTLNAVAFQGTGLVDDLAVADDSDVVSPAAVMLTVNIV
                     GSGSVLTNGAAVANGGEVQSGTAIVPTPDDWHRLHGVTGTEASYDGETGIVTANPNAV
                     DPSVTVTFTNVTGTLAMGEKTVSAEKLAAWATGKNTPALTEAQVSANGAAWYDEYLLN
                     IAKNDLASTIEITSISVDAAEATIELAASSTAVDFTASALNGTLNVYTTDDLGTAFTL
                     AGSFTVTPGENGTATVKVPVAQGNFIKAVVE"
     gene            4259..5734
                     /locus_tag="PK576_06890"
     CDS             4259..5734
                     /locus_tag="PK576_06890"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOE61356.1"
                     /translation="MKRSKRRFGVVALLVLALVLSFPHGAARAAGVLDFRQGFSGVAS
                     KATAAVVFIQVEKQVPAGNMQYYFNNPYDLFGEEFFERFFGRRQPRQQMPRRRQPQQM
                     FKQTGQGSGFLISKDGYILTNTHVVGDVDKITVRLSDGREFAAKRIGADVKTEVALIK
                     IEAKEDLPYLEIGKPEELKIGEWVIAIGNPFGLKETLTVGVVSAKGRSNIGITDYEDF
                     IQTDAAINPGNSGGPLLNIDGKVVGINTAIYSRSGGYMGIGFAVPIDMAVRIKDQLIA
                     TGKVTRGYVGVFLNPGEVTREMAASFGRSEAGGVLIAEVQKDGPADKAGIKSGDILIE
                     LNGKKIVGNTDFRNDVARIMPNEKAEVLLFRDGKTRKVTVTVAAFPEDEQGTDAGGES
                     GGAALLEKLGLQVQDLTQEIAQQLGYENLRGVVISDVMQGSVADEAGLRPGMLILEVN
                     RAEVKNARQFEAAVGKAKGSNVLVRVKTKEGTIFLNLPTQE"
     gene            complement(5870..6802)
                     /locus_tag="PK576_06895"
     CDS             complement(5870..6802)
                     /locus_tag="PK576_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE61357.1"
                     /translation="MPTPVLHSVLPVCLLVLLGAALRHLRFLPDLFFVQLNRLGFWLL
                     LPSLLFSTLANAPSQTSSEGFRISMLLCVSAAGMILAGVVVSCLLRLPEPSARALIQA
                     AMRGNLAYTALPILLYAYGENSAAAATATLAWAPTIPFYNFCAVLILTPKGAGSPHSR
                     FLRVASDILRNPLILGCGAGFIVLWSGLRPPTACMRAIESLGRAALPCALLSLGAGLN
                     FSALRTHGSAALAATALKLLASPALGRALAPAFGVHGDARMIALLYLATPCAVTSYVM
                     ADQMGADRELAASTIALSTLLCIPVMIFLMLCLR"
     gene            complement(6855..7721)
                     /locus_tag="PK576_06900"
     CDS             complement(6855..7721)
                     /locus_tag="PK576_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE61358.1"
                     /translation="MPDPHISSFANPRVKHAVRLRQRSFRDETGQMLIEGYRECKRAL
                     DHGYRPQILFCCEELYLKNLNEPEIVRRCREAGAEILSCSAPVFGKMAYRERPEGLLM
                     IGPHLTRRLEDLRLPDPALIVVAEAVEKPGNLGTILRSADAAGVQAVIVCDRCTDIHN
                     PNVVRASTGTLFSLPVIEADSGDTLAFLHARGFRILATTPHAEPLHTEVPLTGNVAIA
                     VGSEQYGLTEKWMGAADLRVRIPMLGIADSLNVSAAATILLFEALRQRIAAGQARVPP
                     HEAWHGEAMFDA"
     gene            complement(7733..10819)
                     /locus_tag="PK576_06905"
     CDS             complement(7733..10819)
                     /locus_tag="PK576_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucokinase"
                     /protein_id="HOE61359.1"
                     /translation="MSFDLLVLTAAHEAQAAGYRAQLAWRRAVGLLAPETRTLVVADP
                     GGRRVGSLGATLHVLALLAEEAGRVKSVADAFAGRRILICHSGGDSRRTPAYAAQGKV
                     FTPVPTCDDHGTPLALFDLILRNVSALPAPAAGQVLITSGDVLLTFDASAVDLSRPGI
                     TGIAAFGPTERGSRHGVYVPASFCPNPQRAECVPVLDFLQKPTEAEAEARGAVDPFGH
                     VAVDTGLLSLDPAGCARLLAAAGWSRKETGHGKSKTRHVTKGLLAEILAGHSPALDLY
                     EELTMAVSPRYDEARYLTRLVTERGRDAAHSRRMRVFYRAVRPLAFHVNLVPYCDFFH
                     IGSSRELLVGFSELSRTARTYRFENGCGAKIRGGVSERAFLFNARIDCPLQTGRALLE
                     AVHAPSTCPGLFLDGDNLVTGLPPDATVAIRLPPRTGLVCLPVGRSRWAAVVYGLEDD
                     FKTPFGGAKPCLFLNTPMDTWMARTGLTPRRLWRGEREKRDGLWQARLWRIGALNDVL
                     SEAVALAASGTAPQPAPALTKAGTRFSLAELLPRVNHTRLLGGRQELRRQVCLLRITE
                     YLLAHDTLPSAELCGWIRTADEARQTEKALSDLLSNEARDNPLLRARILRLLTLIRER
                     WAGAEAAQESRTRTFAAVAEAVAFHFKLPAAPRPAAILHDQVVWATTPVRIDFAGGWS
                     DTPPICTEQGGTVLNAAVTLNGLYPIQVMAKLNTAGRIRLASIDLGERREFRSAAELL
                     DHHDPTDWAALAKAALVLTGIAPPDARASLSRWLDTLGGGLDLTLFSALPKGSGMGTS
                     SILGAAIIACLEHVLGLPLQSDRLIRMTSILEQRLCTGGGWQDQVGGILPGVKLIRTQ
                     PGPDQTVSLRWTPFDMSEGSELRRRCLLYYTGQKRMARNILHNVVSGYLARDPALLRT
                     VGEIKNLALEVKEALDAQDLDAFAHGLRRYWELKKRIDPGSTNPQIESILNRVARDTQ
                     AALLPGAGGGGFILLLANSAAAAARIRRTLEAAPPNGQARFFDFAVDTQGLKITVL"
BASE COUNT         2335 a         3238 c         3773 g         1947 t
ORIGIN      
        1 cctccaccat ttttcgcgct tgcgccgtga cgggggtttg cgctaacctt atccatccgt
       61 gttacggaaa ggcgttttta catgaagaag gatacccacc ccaactatga tgacgcgacg
      121 atcacgtgtg cctgcggcaa tgtgatcaag acgcgttcca cccgcaaaga gatgaacgtc
      181 aacacctgct cggcctgtca tccgtatttc accgggcagc agaccatgct cgacaccgag
      241 ggtcgcgttg accagttcat gaagcggtac agaatcaaga aggcttagtg cttcggggtc
      301 ggggcgggcc gggggcgttg ccgcggggcg gcagtcgcag ttcctggccc gccttgtctt
      361 ttgcctatgc tggacaaagc ccacatcctt caggctctcg gccgggttca ggctgcggaa
      421 gcggccctct ccgatcccgc tgttctgggg aatcccaggg tgtaccggga aaagcttcgt
      481 gagcatgcgg ccctgaagcg tttggagagc gcggccaacg cctatttcaa gctgttggac
      541 gagctggagg gcaaccgtgc gctggagtcc gagccggggg gagatccgga gttggccgaa
      601 ctggcgcggt cggaaatcgc gcggatcagc gaacgggtgc ccgaagccga gcgcgaagtg
      661 ctggcctgtc tgctgccgtc cgatccgcgc gacgcccgca acgcggtttt cgagatccgc
      721 gcgggaaccg gcggtgagga ggcggcgctg ttcgctgcgg tgctgttccg catgtattcc
      781 aaattctgcg acgcgaaggc gtggaagatc gcggttctgg acgcgcacgc cacggagttg
      841 ggcggctaca aagagatcgt gttcacggtg agcggcgagg gggcctacgg cgcgttcaag
      901 ttcgagagcg ggggccaccg ggtgcagcgc gtgccggaga ccgaggcgca gggccgcatc
      961 cacacctccg ccgccacggt cattgtcttc cccgaagcgg acgaggctga cgagattccg
     1021 cttccggaga gcgagttgag ggtggacatc ttctgcgcgg gcgggcatgg cggtcagggc
     1081 gtgaacacaa cctattcggc gattcgcatc acccacctgc cgaccggact ggtcgcgcag
     1141 tgtcaggacg agcggtcgca gcaccgcaac aaagaaaagg cgctcgcggt gctcaaggcg
     1201 cggattctgg atcagcggcg tcaggccgag gaggccgcgc tcgggcagac ccggttgggt
     1261 ttgcggggat cgggggaccg cagccagcgc atccggacct acaacttccc gcagaaccgg
     1321 atgaccgatc accgcgtcaa tctgacgctc tacagtctgg accggatcgt ggagggagaa
     1381 ctcgggcccc tgttggacgc actgcatgcg tatgatctgg atcagcgtct ggcccaagaa
     1441 tccgggctcc ggccagagta gcggagcggc ctattttccg gccgcgcctt ccgcatccgg
     1501 cggcgcgagc ctgcgccgtc gcacaaacgc gcggtgggcc gccaacaaca tcagcagtac
     1561 cagaaaaaag accgcgatac gcagacgggg atcacgcagg tagggcagga tcgtctcgcc
     1621 gatgaacacc cccagttgca gaaacagaag ggactgcacc acggtggaga gaaaaaccag
     1681 caggagaaaa cgcgggaacg gataccgcac ggctccggcc gcggcattgc tcacggtgcg
     1741 ggctcccggt agaaaacgcg agaccactac ggttttgatg gcgtggtggc ggaagtaatc
     1801 ctccatccac tccagacgcg acggggagac ccagcgcagg cggaacagga gcgaggtcgc
     1861 gtacctcccg atcagataga gaccgatgtc accggacatc ccgcccgccg tcatgcaggc
     1921 gaaagcaaac ccccatccca agtgacccgt agccaccaga aggctcacgc cgcatcgggc
     1981 ggcatcctcg agaaagcagg tcccggccgt caccagcaag gcttggagcc agaggtggcc
     2041 ggcaaggggg gcgcataatt cgacgaatac ggtttcggtc acatcagcct cggcgcgcgc
     2101 aatccgacac gggcctcccc ggaggaatcg gcgcgtcacg tttccccggc ccctgtgccg
     2161 gacgggacgg gcattccgga gttttcagga aaacccgggg ccagtatgac acatcccgct
     2221 tcgggctggc aaggaggggc agaaaaaaaa acgcaaaaaa gtggttttcg ggcttgcttt
     2281 gcgaggcctt ttcggcgtat cctttgacta caacgaaaga atggtcttgt ctggaaacag
     2341 aagaacgggt gtttttctgt ggccggatag gattttgaag gtttagagtc aataacgaag
     2401 gaacacgata ggagggtttc ttaaatgaac ctgacttcaa agcggatgat gatggcggcg
     2461 gcatgcgcgg ggctttgcgc ctttgcgtct atgacgttcg cgggcgaccc gacaaaatgg
     2521 tgggtctccg attcgttcga ggtcgccgat ggcggggccg cggatctccc gatctcacag
     2581 tacaagcaga cggttacgga aatcgtgggt ggcgggggtg caacgacaac caacttcctc
     2641 tgggttgcgg atggattgga cgcatccaag atcatcgcga ccaactccta cattgggcag
     2701 ccttttatca ccaactctgg gctcagggag ttggttctgg aactcgcgac cgagggcagc
     2761 accctcacgc ggcacgtgat cggggataac ggtgtttcga cgggcaaggc gttctctgca
     2821 tcagcgccgg tctatgtcga cacgatgatc cagttcaggc cgagcgagga cacgcccacg
     2881 atcggagatg acgtcaagat tgcggtctac gtgaacgtca actcgaatct ggtggtttac
     2941 catgggatgg atggggctga tccgaccaac agtgtgtttg agacagaatt catcaatcct
     3001 gatacatggt accgtctgac tattgaaatg acggggtatg atgaatggac cccggcgtgt
     3061 aaggtttatc ttaataagat acaactgtct catgcgaacg cgcttgaggg tggcctcttc
     3121 ctgactgcgg gtcccggcgg cacgctcaac gccgtcgctt tccagggaac cggtctggtg
     3181 gatgacctgg cggtggcgga cgactccgac gtggtctctc ctgctgcggt tatgctgacg
     3241 gtgaatatcg tgggttcggg atccgtcttg accaacggtg cggcggtcgc caacggcggc
     3301 gaggtgcaga gcggaacggc catcgtgcca acccccgatg actggcatcg gctccatggc
     3361 gtcacgggta ctgaagcgag ctatgacggc gagaccggca tagtgaccgc caacccgaat
     3421 gcggttgacc cgagtgtcac ggtgaccttc acgaatgtca ccggcaccct cgccatgggc
     3481 gaaaaaacgg ttagcgccga gaagctggcg gcatgggcca ctggaaagaa tactccggcg
     3541 ctcaccgaag cgcaggtttc ggcgaatggc gcagcgtggt acgacgagta cctgctgaac
     3601 attgcaaaga atgatttggc cagcaccatc gagatcacct caatctccgt ggatgcagca
     3661 gaagcgacca ttgaattggc agcctcttcg actgcggtcg acttcactgc cagcgctctc
     3721 aacggaacgc tgaacgtcta caccacggat gatctgggaa ctgccttcac cctggcggga
     3781 tccttcaccg tcacccccgg agagaatgga acagcgactg tcaaggttcc tgtggctcag
     3841 gggaacttca tcaaggcagt ggttgaataa ccggttctga ccgagagttg atacaagaca
     3901 tgcaggggtc cgcggaaacg cgggcccctg ttgttttatg cgacaacctg acgcggtgaa
     3961 aaggacaagg tgtcccgtgt gaaaagcaac gatgattcat aaatgataga aaaggctaat
     4021 ttaaggcgta aaatgttata tattcactcg aatggatcga aaaacaagtt atattgaata
     4081 tggcatggtt ggtgctttaa gcaaatcggc ttagcaagag gcggcaagag gtaacacaaa
     4141 acaggtccgt agaggccata atctcatacg cttgcgaagt cgggatggaa aaagagcggc
     4201 ttgccgccac cgcatgaggt tatggccttt tccatccgcg ggatgaggag gaaagagtat
     4261 gaaacggagt aaacgtaggt tcggcgtcgt ggctttgctg gttctggccc tggttttgtc
     4321 tttcccgcac ggggcggcga gggcggcagg ggtgttggat ttcaggcagg ggttttcagg
     4381 tgtcgcatcc aaggccacgg cggcggtggt ctttatccag gtggagaaac aggttccggc
     4441 cggcaatatg cagtattact ttaacaaccc ctatgacctt ttcggggagg agttcttcga
     4501 gaggttcttc gggcgacggc agccccggca gcagatgccg cggcggcgcc agccgcaaca
     4561 gatgttcaag cagaccggac agggttccgg gttcctgatc agcaaggacg gctatatcct
     4621 gaccaacacc cacgttgtcg gagatgtcga taagatcacg gtgcgcctgt ctgacggccg
     4681 ggaattcgcc gcaaaacgga ttggcgccga cgtgaaaacc gaggtcgcgc tgatcaaaat
     4741 cgaggcgaag gaggatctgc cctatctgga gatcggcaag ccggaagagt tgaaaatcgg
     4801 cgaatgggtg atcgccatcg gcaacccgtt cggactgaag gagacattga ccgtgggggt
     4861 ggtgagtgct aaaggacgca gtaacatcgg gatcacggat tacgaggact tcattcagac
     4921 ggacgccgcg atcaatccgg gaaactcggg cggccccctg ctgaatatcg acggcaaggt
     4981 cgtcgggatc aacaccgcaa tctacagccg cagcggcgga tacatgggga tcggtttcgc
     5041 ggtgcccatc gacatggcgg tcagaatcaa ggaccaactc atcgcaactg gcaaagtgac
     5101 aaggggttat gtcggcgtgt tcctcaatcc cggcgaggtc acccgggaga tggcggcatc
     5161 cttcgggaga tcggaagcgg gcggagtcct gatcgccgag gttcagaagg acggtcctgc
     5221 ggataaggcg gggatcaaga gcggggatat cctgatcgaa ctgaacggca agaaaattgt
     5281 ggggaacacc gacttcagaa acgatgtggc acgaatcatg cctaatgaaa aagcggaagt
     5341 gctgctgttc cgtgacggca agacgcgtaa ggtcacggtg acggttgcgg ctttccccga
     5401 ggatgagcag ggaacggatg ccggaggcga atccggtggg gcggccctgc tggagaaact
     5461 cggactgcag gttcaggatc tgacgcaaga gatcgcgcag cagctgggtt acgaaaatct
     5521 gcggggcgtc gtgatttcgg atgtgatgca gggctcggtt gcggatgagg cggggttgag
     5581 gcccggaatg ctgattctgg aggtcaaccg ggcggaggtg aagaacgcga gacaatttga
     5641 ggcggcggtc ggaaaagcga aaggaagtaa cgtgctggtg cgcgtgaaga ccaaggaggg
     5701 cacgatcttc ctgaatttgc cgacccaaga gtaacgatca accacacaac ccaagtatat
     5761 atagaccact cctgtcatgc agcaccgggg ccgggtaaaa ccggccccgg tttttttgcg
     5821 aacaaatgcc ggttataggg ttgcgggctt tatccgcgtg gaacccgcgt cagcggagac
     5881 aaagcatcag gaaaatcatc accggtatgc agagcagcgt gctcaacgcg atggtggaag
     5941 ccgccagttc gcggtcagcc cccatctggt cagccatgac gtaggaggtt acggcacaag
     6001 gggtggccag atacaggagc gcgatcatcc gcgcatcgcc atgcacgccg aacgcgggcg
     6061 ccaaggcccg gccgagggcg ggagaggcca gcaatttgag tgcggtcgcc gcgagtgcgg
     6121 cgctaccgtg ggtacgcagc gcggagaagt tcaggcccgc acccagagag agcagtgcgc
     6181 agggcaacgc ggcgcggccg agagattcga tggcccgcat gcaggcggtc ggcgggcgga
     6241 gaccgctcca cagcacgata aagcccgcgc cgcagccgag gatcagcggg ttgcgcagga
     6301 tgtcggaggc gacgcgcaag aaacgggagt gcggcgagcc cgcgcctttg ggggtgagaa
     6361 tcagcacggc acagaaattg taaaacggga tggtcggcgc ccaggccagg gttgcggttg
     6421 ccgcagccgc actgttttca ccatacgcgt agagcagaat gggtagcgcg gtataggcga
     6481 ggttgccccg catggcggcc tggatcaggg cgcgggcaga gggttccggc aggcgcagca
     6541 gacacgacac caccacgccg gccagaatca tgcccgccgc gctgacgcag agcagcatgg
     6601 agatgcggaa gccttcgctg ctggtttgcg aaggcgcgtt cgcgagggtg ctgaacaaca
     6661 gtgaaggcaa gagcagccag aaccccagac ggttcagctg cacgaaaaaa agatcgggca
     6721 gaaaccgcag atggcgaagc gccgccccga gcaggaccag caggcagacg ggcaacacgg
     6781 aatggaggac cggggtaggc atatttcgcg cacagtagta tagcggcctg tcacgcggcg
     6841 gcgcaaggca ggattcaggc atcgaacatc gcttcgccat gccatgcctc gtgcggcggc
     6901 acgcgcgcct gtcctgcggc gattcgttgc cgcaacgctt cgaacagaag aatggtggcg
     6961 gcggcggaaa cgttcagcga atcggcgatg cccagcatcg ggatgcgcac gcgcagatcg
     7021 gccgcgccca tccatttttc ggtcagcccg tactgttccg accccacggc aatcgccacg
     7081 ttgccggtca gcgggacctc ggtatgcagg ggttccgcgt ggggcgtggt tgccagaatg
     7141 cggaacccgc gggcgtgcag gaaagccagc gtgtccccgc tgtcggcttc gatcaccggc
     7201 agggagaaaa gcgtaccggt cgaggcgcgc accacgttgg ggttatggat atcggtgcaa
     7261 cggtcgcaca cgatcaccgc ctgcacacct gcggcatcgg cggaacgcag gatggtgccc
     7321 agattgccgg gtttctcaac ggcttccgcc accacgatca gcgcggggtc gggaagtcgg
     7381 agatcctcga gtcggcgggt caagtggggg ccgatcatca aaagaccttc gggacgttcg
     7441 cggtaggcca tcttcccgaa gaccggtgcg gagcaggaaa ggatctcagc cccggcttcc
     7501 cggcagcggc ggacgatttc aggctcgttg agatttttca ggtaaagttc ctcgcaacaa
     7561 aagaggatct ggggccggta cccgtgatcc agggcgcgtt tgcattcgcg gtagccttca
     7621 atcagcatct ggccggtttc atcgcggaac gagcgctggc ggagccggac cgcatgcttg
     7681 acgcgcggat tcgcgaacga ggagatatgc ggatcaggca tggggttccc tgttacaata
     7741 ccgtgatctt gagcccctgg gtgtccacgg cgaagtcgaa gaaacgcgcc tgcccgttcg
     7801 gcggggcggc ttccaacgtg cggcggatgc gcgccgcggc cgcggcactg ttggcgagca
     7861 ggaggatgaa cccgccgccg cccgcgccgg gcaacagcgc ggcctgcgtg tcccgggcca
     7921 cgcggttcag aatcgattcg atctgcggat tggtcgagcc cgggtcgatg cgtttcttca
     7981 gttcccagta gcgccgcaac ccgtgcgcga acgcatcgag atcctgggcg tcgagggcct
     8041 ctttgacctc cagcgcgagg tttttgatct cgcccacggt ccgcaacagc gccggatcgc
     8101 gcgcgagata accggacacc acgttgtgca gaatgttacg cgccatgcgt ttctggccgg
     8161 tgtaataaag caggcagcgg cgtctgagtt cactgccttc cgacatatcg aagggggtcc
     8221 agcgtaagga cacggtctga tccggtccgg gttgcgtacg gatcagtttc acgccgggca
     8281 ggattccgcc gacctggtcc tgccagccgc caccggtgca caggcgctgt tccagaatcg
     8341 aggtcatccg gatcaggcgg tcggattgca acggcagtcc cagcacatgt tcgagacagg
     8401 cgataatcgc agccccgaga atcgagctcg tgcccatgcc cgaccctttg ggcagggcgg
     8461 agaagagggt caggtccagt cccccgccca gcgtgtcgag ccaccgggac agcgacgccc
     8521 gcgcgtcagg cggggcgatg ccggtcagga cgagggcagc tttggccagt gccgcccaat
     8581 cggtcggatc gtgatgatcg agcagttcgg cggcgctgcg gaactcccgc cgctcgccca
     8641 gatcgatact ggcgagccgg atccggcccg cggtgttcag tttggccatg acctgaatcg
     8701 ggtagaggcc gttgagcgtg acagcggcat tgagcaccgt gccgccctgt tccgtgcaga
     8761 tcggcggcgt atcggaccag ccgcccgcga aatcgatacg caccggtgtg gtcgcccaga
     8821 ccacctgatc gtggagaatc gcggcgggac gcggtgcggc gggaagcttg aaatggaacg
     8881 ccaccgcctc ggccactgcg gcaaaggtgc gggtccggga ttcctgcgcc gcttccgctc
     8941 cggcccagcg ttcccggatc agggtgagca gacgcagaat gcgcgcgcgc agaagcgggt
     9001 tgtcgcgggc ttcgtttgag agcagatcgg acagtgcctt ttcagtttgc cgggcctcat
     9061 ccgcagtgcg gatccatccg cacagttccg cagacggcag cgtgtcatgg gccagcaggt
     9121 attcggtgat gcgcaggagg cacacctgcc gccgcaattc ctgccgcccc cccagcagac
     9181 gggtgtggtt cacgcgcggc agcaactccg cgaggctgaa gcgcgttccg gcttttgtga
     9241 gcgcgggagc gggttgcggt gcggtgccgg atgcggcgag ggccacggct tcggacaaga
     9301 catcgttcaa cgcgccgata cgccacaacc gcgcctgcca gagcccgtcg cgtttttcgc
     9361 gctccccgcg ccatagccgc cgcggcgtca ggccggtgcg cgccatccac gtatccatgg
     9421 gcgtgttgag gaacaggcag ggcttcgcgc cgccgaacgg ggttttgaaa tcgtcctcaa
     9481 gtccgtagac cacggcggcc caccggctac gtccgaccgg caggcagacg agacctgttc
     9541 gcggcggcag ccggatggcc acggttgcgt cgggcggcag tccggtcaca agattgtcgc
     9601 catcgaggaa aaggccgggg catgtgctcg gggcgtggac cgcttcgagc agcgcgcggc
     9661 cggtctgaag ggggcagtcg atgcgcgcat tgaacaggaa ggcgcgttcg gacactccgc
     9721 cgcggatttt ggcgccgcag ccgttttcaa accggtatgt ccgggcggta cgcgagagtt
     9781 ccgagaagcc cacgagcaac tcccggctgc tgccgatgtg gaaaaaatcg cagtagggaa
     9841 cgagattcac gtggaacgcc agcggccgta cggctcggta aaagacgcgc atacggcggc
     9901 tgtgggccgc atcccggccg cgttccgtca ccaggcgggt gagataacgc gcttcatcgt
     9961 aacgcggcga gaccgccatg gtcagctctt catacagatc aagcgccgga ctgtgtcccg
    10021 cgaggatctc cgccagcagg cctttcgtca cgtgacgcgt cttggacttt ccgtgtcccg
    10081 tttctttcct gctccatcct gcggcggcca gcagccgcgc gcagcctgcg gggtcaaggc
    10141 tcagcagacc ggtatccact gccacatgcc cgaaggggtc caccgcgcca cgggcttccg
    10201 cctccgcttc tgtgggtttc tgcaggaaat cgaggaccgg cacgcattcg gcgcgctgcg
    10261 ggttgggaca gaaggaggcc ggcacgtaca cgccgtgacg cgaaccgcgt tctgtcgggc
    10321 cgaacgcggc gatgcctgtg atgcccggcc gcgagagatc cacggccgag gcgtcgaaag
    10381 tcagcagcac atcgcctgag gtgatcagca cctggcccgc cgcgggggcg ggcagggccg
    10441 agacgttgcg caggatcaga tcaaagagcg ccagcggcgt accgtgatcg tcacacgtcg
    10501 gaaccggcgt gaagaccttg ccttgcgccg cataggccgg agtgcgtcgg ctgtcgcccc
    10561 ccgagtggca gatcagaatg cgtcgtccgg caaaggcgtc ggcaaccgat ttgacgcgcc
    10621 ccgcctcttc cgcaagcagc gccagcacat ggagcgttgc gccgagcgag cccacccgcc
    10681 tgccgccggg atcagcaacc acgagcgtgc gggtctcggg cgcgagcaga ccgacagcgc
    10741 gtcgccacgc caactgtgcg cggtagccgg cggcttgcgc ctcatgggcc gcggtcaaaa
    10801 caagcagatc aaaagacatg catcaacggt atcatgtccc gctaaaaccc gtcaaccgga
    10861 tgcgagggca aaacgagcat ttctgcaatc cgagcctgat acgaaaaaga accgcgattt
    10921 ttttatgcgc ggtgttgact tgcggggccg ggggtgatac tgtacgccgc tctttcacgc
    10981 cacacggggt tctgcggaat cgtcgcgtgg cgtggggggg gcggcgaaaa aagtgatttt
    11041 tttcgccgca gggggttgac agcatgcggt tgattctgct aatgtctttc cccgcgccgc
    11101 aaatcggcgg gaaaccccga aggcggcatg agaagctctt tgaaaaccgg aagctcaagc
    11161 gcagacccga cggataccga tccggagggg atgcggaaaa acagaagctt gtgtttgtcc
    11221 ggaatcaatt aagaacagaa cgcgccgcgg cctttcgtgg ctgtcggcgc gcgacggatt
    11281 tttcttgaga gtt
//
LOCUS       DAOSLE010000084        11065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5835_length_11065_cov_8.812171, whole genome shotgun sequence.
ACCESSION   DAOSLE010000084 DAOSLE010000000
VERSION     DAOSLE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_11065_cov_8.812171"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK576_06910"
     CDS             complement(<1..544)
                     /locus_tag="PK576_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61360.1"
                     /translation="MSAALAIHDLRVAYRAGDSEVLRGISLTLAPGEHCALLGLNGSG
                     KTTLLRAVAGLQPFSGTVTVCGIALRRETVRDVRDRIGFLFSTPDDQLLFPQVLADVS
                     FALERAGRPQAEAREAALQTLEALGIAAYAEHAPHTLSQGQRQRVALAGALVAQPPLL
                     LLDEPTASLDPVGRDELAQVL"
     gene            complement(541..1293)
                     /locus_tag="PK576_06915"
     CDS             complement(541..1293)
                     /locus_tag="PK576_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOE61361.1"
                     /translation="MIAFSEAFGGGNGAARRLAPAVRLLCGAWVFVCCAVVPLGSPAG
                     IALFVVAWLAWLLGCAMPPRQLALSLGFAICLFAPLLLLAPVARWNGSAVTWTEALRA
                     PLYVGARGVAGVTICVGALSLLPFTEFTDAVAALPLPRAVTGLLVQIVHQAALLTDET
                     RRMADAMRVRGASSARLAMRLRVVTAFPANWMTRLALRAARAGDAMEVRGFDGLPPNM
                     PTPTFSRSDWLALTATTLLAVGALALRWRGCA"
     gene            complement(1290..1916)
                     /locus_tag="PK576_06920"
     CDS             complement(1290..1916)
                     /locus_tag="PK576_06920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE61362.1"
                     /translation="MTNPPCPDPRQSFFDNIADVWDGWHDLPALNARLAEFFERVGLQ
                     PDESVVDVGCGTGNLTLALLARLGPQGRVAALDISPAMLARAASKTTDERVTWHEAPA
                     DRIPLPNGTCDRVFCFSAWPHFDRPDHVVAEFRRVLRPGGQAHILHLISKEEVNRIHS
                     EAHPTVRADRLPPVDDVADLFRANGFTVIETEDNASRYLLSVRKEPEA"
     gene            complement(1960..2709)
                     /locus_tag="PK576_06925"
     CDS             complement(1960..2709)
                     /locus_tag="PK576_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61363.1"
                     /translation="MIQPREYAFCGLFGAAALLLPTLFHLLHLGHVFMPMYLPLVALA
                     FFVRSGAAALTALLVPLLSGAVTGMPPFMPPIAPVMALELALMALMIGTLRQAVPRMP
                     VWCLLALALAVGRIVNALLLYTAARVLELPAGFVAGISLLSGWPGLLLMLAVIPGLVQ
                     ATGYAQCHPHNSGACNRSGTGECEDNAFLVKRGAESRERGRPRPQQRVLQKIDAPTSF
                     GNQTAHRNHERPFAERARTPALPTAHTQDTR"
     gene            complement(2754..4679)
                     /locus_tag="PK576_06930"
     CDS             complement(2754..4679)
                     /locus_tag="PK576_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOE61364.1"
                     /translation="MDNGTLTKVLIAAYGAGVLTVVAQTATNNADTARAPEIEIEVTA
                     SPITQLEETTLDGANRVVVGRDQLDRLSATDLPTALRQVPGVSISRYSPVGAYGGAQG
                     GSVYVRGAGTARPGGEVRIYSDGVPRESGVWSHPLMDIVPIDFTERITVAKNPQPQHY
                     AGTFGAVDVETRRRKTEGHEGEVSAAYGRFNTLISSAAAGGKIDLFDYYAGAAYKYSE
                     GARQHGEAELQSAFARTGWELSPHDHLSYIFLGTDNWVRDPGPKGGPKPQRDQFNTET
                     LTHAVRLETQRDRIRGYSLIYYEEGEIRWHKDHLSDGVLTSPAGFSNTDWDNYGFRSS
                     YDILLLDRLTLTGSLDSWSEGGETWNIRSSDNRRVWGYNGRFFTTAPYLGARYEFDVG
                     HDWTLTPSAGARHYVSSEFDNETAPCAALTLSREGLSVFVSHARGVHYPGVYIRGVSP
                     STWRSLEAETLDTTEAGVQMEISEQATLHATLFHTEVDNRMDSTSAGYFNTGDMKAQG
                     AEVSMHFYPRNDLSFFVGGTYTDPETHPVSRMPEVTFTAGSSYRITHALRWDLDAQYV
                     TTQYAYSMRSINPDLEKLDDYLAVNTRLSLDLRALCPLDGELYVALENLTNRHYEYFP
                     DYPMPGIMWYTGMKVKF"
     gene            complement(5142..7211)
                     /gene="dnaG"
                     /locus_tag="PK576_06935"
     CDS             complement(5142..7211)
                     /gene="dnaG"
                     /locus_tag="PK576_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOE61365.1"
                     /translation="MAVRITESVVEEIRARVDIVDVIGSRVPLKKAGASFKACCPFHH
                     EKTPSFTVNPTRQSFYCFGCGEKGDVFSFVMKQDGLTFADAVKAMAERVGVVLQDRDD
                     QGSSGERAVLYAAHAAVAEYYRQCLLNLPEAEHARRYLAQRKLSDEIVKSFGIGYAPE
                     HAVEALLNALEKNRFPPEVLVRAGILCESDRGGPGRYFDRFHGRLMFPIWDRQGRVVA
                     FSGRLLTPRERVGKYVNSPETPIFTKSRVLYALHKASGKIVKHPRREAIVCEGQIDVI
                     RCHASGFETAVASQGTAFTRDHVKLLKRYADSVTLVFDGDAAGRKAALHTGTLFLEEE
                     IPVRVACLPVGEDPDSILRDQGPEPFRDLLERAVSLIAFQIETQMQDEPNPEAIDAVS
                     RVARGVIGTLATCPGGVLRTLLLQEAGERLHLPYSALEKDLEKQLEVLSRKAALAQTF
                     KTDGGAADPSGERRDEQCAGSGPSPEYNADPDIDPAFAPPSRLETLLCECLIEHERDP
                     AVFELVSRYLPLDLITHPFARDLVVVLLRQVQSGEDMLAGYYQQTIPEWIPLFDRLLA
                     HKQKMLCAREMTQEDAVQDLIRQLWIDRLKAEQQALGTESSPECDARRYTLSLRMKAL
                     RNRPWAQVCDLLAELGAPGATGSGIPAEAASAGAESCAHATDSASAVSDPEEFPPSAY
                     LPDEMPD"
     gene            7533..9413
                     /locus_tag="PK576_06940"
     CDS             7533..9413
                     /locus_tag="PK576_06940"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE61366.1"
                     /translation="MSTVYGRGGHPGRTRNAGWSRVKNDAIEQRGFQLLEQLDETSRT
                     QVWRAVQTNLDRTVILQILKEHAAQNPGETAHFLMIARRIARIKSEAVCALFDIVSEA
                     PLHYVVMEQVDGPTLEELVLQHGPLPFPQILRIAASLADGLDQMWRSAHLVHRNLKGA
                     TIRLDERGIAKITDFSLAIEAGPGIDATDMDQGLIVGTPCFLSPEQASGSRTLTTQSD
                     MYALGALLYYLATGHVPFEELDVASILAAHCNGRIPPPHLLRNDLPKVFSWFLHRLMM
                     KSPENRYAGWDAVRQDLDRLRKGTSPASCVRPDDTAPSTVDAAPLREAVSDTPAAEQE
                     AAGPRIRMAGRPKNEKITAYQSKTIEEDHAREIRRETLVREGFCWLVLAVWLIGLFWV
                     RAVLEPAEGQPDARPAPEPPPIDETQAGDKTPEAEKGKASTDVPETSDVPVPPPSSAT
                     GTATDLPAVGEMHPALKTALSQALIKGNLIRARALVQSHPDPFRNQAELAAFLTDMPD
                     PDALVAEYLLDQTGKPLQWERNGKTRTVIPRAIVGSTAHLDANGNRIAVPLDTLSPEE
                     KLRWMNPARTAHQTAAQCLLLLHSPRRHEIRSKMAHCPLLAPFLDEALRLMPEGVTPP
                     AE"
     gene            complement(9394..10233)
                     /locus_tag="PK576_06945"
     CDS             complement(9394..10233)
                     /locus_tag="PK576_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE61367.1"
                     /translation="MRLVVNGIEIPEEVVTREAERLRHPYEEYVRANGGEPDEAQLRE
                     WAVEGVIESELVRQEACATQPIPSEARIRQELTQYAETYQPYPEAERAARARGGLQVR
                     RYLKQIRKDVPVPDEAAARNYFDTHAELFKVAEGVRLEHVCRFVDLASRPSVYMSLLQ
                     LKSDVEAGRVDWRGEAVPYYSDTYNRDGGAFAVVTRGELPEAIERDLFALQPGQWSDV
                     IDFGNGTLHLFKVTERLLPRALSFEEVRETVIQSLYRVAAEEAVYARIDELKNKASIQ
                     REA"
     gene            complement(10257..>11065)
                     /locus_tag="PK576_06950"
     CDS             complement(10257..>11065)
                     /locus_tag="PK576_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE61368.1"
                     /translation="VSIAAMFMAGILPGILVGVCIMIAAAVIAIRHGYGKAVPDAEAE
                     PRQNPLIVCWRAIPSLLMMILIIGGILLGIFTATEAAAVSVAYAFVLCVVIYREIPWK
                     DLPGICLQTGITTAVVMLLIGSSSAMSWIMTVANIPQSVSSALMGLSENPVVILLTIN
                     ALLLVVGMFMDMTPAILIFTPILLPVVRQLGLSDIHFGIILIANLCIGLCTPPVGTCL
                     FIGCGVGKTTIAKVTVKALPFFAAMIIALMLITFVPWISMAIPKASGLLK"
BASE COUNT         2202 a         3555 c         3388 g         1920 t
ORIGIN      
        1 tcagcacctg tgcgagttca tcgcgtccga ccggatccag cgaggcggtg ggttcatcca
       61 gcaacagcag cggcggttgc gcaaccagtg cccccgccaa cgccacccgc tggcgctggc
      121 cttgcgacag ggtgtgcggc gcgtgctcgg cgtacgccgc aattcccagc gcctctaacg
      181 tttgaagcgc cgcttcgcgc gcttctgcct gcggacgacc ggctcgttcc agagcaaacg
      241 acacgtcggc caatacctgc ggaaacagga gctggtcgtc gggcgtactg aagagaaatc
      301 cgatccggtc gcgcacgtcg cgcaccgtct cgcgccggag tgcgataccg cagaccgtga
      361 ccgttccgct gaagggctgc agccccgcga ccgcacgcag caacgtggtc ttgcccgagc
      421 cgttgagccc cagcagggcg cagtgttcgc cgggtgcgag cgtcagggag atgccgcgca
      481 gaacttcgga gtcgcccgca cggtaggcga cgcgcagatc atggatggcg agtgccgcgc
      541 tcatgcgcat cctctccagc gcagcgcgag cgcacccacc gcgagcaggg tggtggcggt
      601 cagcgccagc cagtcagaac gggaaaaagt cggggtcggc atgttcggcg gcagaccgtc
      661 aaaaccgcgc acctccatcg cgtcgcctgc gcgcgcggca cgcagcgcca gccgcgtcat
      721 ccaattggcc gggaacgccg tcaccacgcg tagtcgcata gcgagccgcg ccgaggatgc
      781 gccgcgcacc cgcatggcat cggccatgcg ccgcgtttca tcggtcagca acgcggcctg
      841 atgcacgatc tgcaccagca ggccggttac ggcgcgcggc aagggcaagg ccgccaccgc
      901 gtcggtgaac tcggtgaacg ggagcaacga caatgcgccg acgcatatcg tgactcccgc
      961 cacgccgcgc gcaccgacat atagcggtgc ccgcaacgct tcggtccacg ttacggcgga
     1021 gccgttccat cgcgcgacgg gcgccagcag tagcaacggc gcgaaaagac agatggcgaa
     1081 gcccaacgac agggcaagct gtcgcggcgg catggcgcaa ccgagcagcc aggccagcca
     1141 cgcgacaaca aagagcgcga tacccgccgg ggaaccgagc gggacgaccg cgcagcagac
     1201 gaagacccac gcgccgcata acaggcgcac ggcgggggca agccgccgcg ccgcgccgtt
     1261 gccgccgccg aaggcttcgc tgaaagcgat catgcctccg gctccttgcg cacgctcagc
     1321 aggtaacgtg atgcgttgtc ttcggtctcg atcactgtga aaccgttcgc tcggaaaaga
     1381 tcggccacat cgtcaaccgg cggcaggcgg tcggcgcgga cggtgggatg ggcctcgctg
     1441 tgaatgcggt tgacctcttc cttggagatc agatgcagga tgtgggcctg accgccggga
     1501 cgcagcacgc ggcgaaactc ggcgaccacg tggtccggac gatcgaaatg gggccaggcg
     1561 gaaaagcaga agacgcggtc gcatgttccg tttggcagcg gtatgcggtc ggccggtgcc
     1621 tcgtgccacg tcacgcgctc gtcggttgtc ttgcttgcgg cgcgcgccag catggccggc
     1681 gagatatcga gcgcggccac ccgtccttgc gggccgagcc gcgcaagcaa agcgagcgtc
     1741 aggttgccgg tgccgcagcc gacatccacg accgactcgt ctggttgcag gccgacacgc
     1801 tcaaagaatt cggccagtcg cgcgttgagc gcgggcaggt cgtgccagcc gtcccagacg
     1861 tcggcgatgt tgtcgaaaaa ggattgtcgc ggatcagggc acggcggatt cgtcatagga
     1921 ggctccagct aatcggttcg tgacaggggg catgcgagtt tagcgcgtgt cttgtgtatg
     1981 cgcagtcggg agcgcgggcg tcctcgcccg ctctgcgaaa ggccgctcgt gattccgatg
     2041 cgcggtttgg tttccaaagg aagtgggcgc atcaatcttt tgcaagacac gttgttgcgg
     2101 gcgaggacgc ccgcgctccc gactctctgc tccccttttc accagaaacg cattgtcttc
     2161 gcactcccct gtccctgacc gattacaggc accggagtta tgggggtgac attgtgcata
     2221 accggtggcc tgcacaagac cgggaatgac cgccaacatc agcagcaggc ccggccagcc
     2281 gctgagcagc gagatgccgg ccacgaaacc ggccggcagt tccaagacgc gcgcggcggt
     2341 gtagagcagt aacgcattca caatccgtcc gacagccagc gcgagcgcga gcagacacca
     2401 gaccggcatg cgcggcaccg cttggcgcaa cgtgccgatc atcagcgcca tcagcgccag
     2461 ttccagcgcc atcaccggag caatgggcgg catgaagggc ggcatgccgg tgaccgcgcc
     2521 ggagaggagc ggcaccaaca gagccgtcag cgcggccgcg ccggaacgca cgaagaaggc
     2581 caacgccacc agcggcagat acatcggcat gaagacatgc cccagatgca gcagatggaa
     2641 aagcgtcggc agcaacagcg ccgccgcgcc gaacaggccg caaaaggcat attccctagg
     2701 ctgaatcatc cgtgtcgtga caatctggtt ccaccgaaat cggtatcgtc tttttagaac
     2761 ttcaccttca tgccagtgta ccacatgatg cccggcatcg ggtagtccgg gaaatattca
     2821 taatgccggt tggtcagatt ctcaagggcc acatacagtt caccgtccaa cgggcaaagc
     2881 gcgcgcaagt ccagcgaaag acgcgtgttg acagccagat agtcatcaag cttttccaga
     2941 tcggggttga tcgagcgcat gctgtaggcg tactgcgtgg tgacgtattg ggcgtccaga
     3001 tcccagcgga gcgcatgcgt gatacggtag gaactgcccg ctgtgaaggt gacctccggc
     3061 atacgcgaga ccggatgggt ctcgggatcg gtgtaggtgc cgcccacgaa gaacgagagg
     3121 tcattgcgcg gatagaaatg catcgaaact tccgcgccct gcgccttcat gtccccggta
     3181 ttgaaataac cggctgaggt cgagtccatg cggttgtcga cctcggtgtg gaacagcgtg
     3241 gcatgcagcg tcgcctgttc gctgatctcc atttggacgc ctgcttcggt ggtgtccagc
     3301 gtctcggcct ccagcgagcg ccaagtcgag ggcgaaactc cgcggatata aacgccgggg
     3361 taatggacgc cccgcgcatg ggaaacaaag acgctcagcc cctcgcggga gagggtcaac
     3421 gcggcgcagg gtgcggtttc attgtcgaac tcgctgctaa cgtagtggcg cgcaccggcc
     3481 gaaggcgtaa gggtccagtc gtggcccacg tcgaactcat aacgcgcacc gaggtagggg
     3541 gccgtggtaa agaagcgtcc gttgtaaccc cagacccgcc gattatcgga tgagcggatg
     3601 ttccaagtct cgccgccttc ggaccacgag tccagcgatc cggtgagcgt gaggcgatcc
     3661 agcaacagaa tgtcatacga tgagcggaat ccgtagttgt cccagtcggt gttggaaaag
     3721 ccggccggcg aagtcaggac gccgtcagac agatgatcct tgtgccagcg gatttcaccc
     3781 tcctcgtagt agatcaagga gtatccgcgg atacggtcgc gttgggtttc gaggcgaacc
     3841 gcgtgggtca aggtctcggt gttgaactgg tcgcgctgcg gcttcggacc gcctttcgga
     3901 ccgggatcac gcacccagtt gtcggtgccc agaaagatat atgagagatg gtcgtgcgga
     3961 gagagttccc atccggtccg tgcgaaggca ctctgcagtt cggcctcgcc atgctggcgc
     4021 gcgccttccg agtatttgta ggcggccccg gcgtaataat cgaacaggtc gatcttgccg
     4081 ccagccgcgg cagaactgat cagcgtgttg aagcggccgt aagcggcgga cacctcgccc
     4141 tcgtgtccct cggtcttcct cctgcgggtc tcgacgtcca ccgcgccgaa ggtcccggcg
     4201 taatgctgcg gctgcgggtt cttggcgacc gtgatgcgct cggtaaaatc aatgggcacg
     4261 atgtccatca acgggtgcga ccagacaccg gattcgcgcg gcacgccgtc gctatagatc
     4321 cggacctcgc cgccgggacg tgccgtgccc gctccgcgga cgtagaccga accgccctgt
     4381 gcgccgccgt aggcgccgac cgggctgtag cgtgagatcg agacaccggg cacttggcgc
     4441 agggcggtcg gcaggtcggt ggcgctcagg cgatcgagtt gatcacgtcc cacgaccacg
     4501 cggttcgcgc catcgagggt ggtctcttca agctgcgtga tgggcgacgc cgtcacctcg
     4561 atttcaatct ctggcgcacg cgccgtgtcc gcattgttgg tcgccgtctg ggctaccaca
     4621 gtcaaaaccc ccgcgccgta tgctgcgatc aacactttgg tgagtgtgcc gttatccatt
     4681 gatccctcgc ctcctctttc catcggtcgt gttacaattt caagaaacgt aacacaatgg
     4741 cggcgacagg tcaaggacgg gcgcaagaca aaatgaactg cgggcaatat cacgccattc
     4801 aggcactgga cgcggggacg gggaacgggt attcttgtga caaacgtgag gagaagttat
     4861 gatccccgac cttcaggcga gtctgttgtg cgacgatgtg cggcaggagc gtaacggaaa
     4921 attcatgctg atcggcattt tcgacgggct ggtgcaagcc gtgaatcaac cggtctgccc
     4981 gcgcatttgc ctggtgaacc gctggtgcgc cggagcgggc accttcaccc agcggtcacg
     5041 cattgtggca cctgacgggt tgacgcttgt gggagaaggg caaccggtgc cgatcacgct
     5101 ctctaacgac cagcaggtcg caaccaccgt ggaggtcttt ctcaatctgg catttcatca
     5161 ggcaggtacg cactgggtgg aaattcttct ggatcagaaa ctgcggatgc gctatccgtt
     5221 gcatgtgcgc aagattcagc cccagccgac gccgcctccg ccgggatccc cgaacccgta
     5281 gcgcccggcg cgcccagctc cgccaacagg tcgcacacct gcgcccacgg ccggttgcgt
     5341 aacgctttca tgcgcaaaga cagggtatac cgccgggcgt cgcattccgg cgaggattcc
     5401 gtccccagag cctgctgctc cgctttgagc cgatcaatcc acaattgccg gatcaagtcc
     5461 tgaaccgcat cctcttgggt catctcgcgg gcgcaaagca tcttctgctt gtgcgcgagc
     5521 agacggtcga acagcgggat ccactccggt atggtttgct gataatatcc ggccagcatg
     5581 tcctcgccgc tctgaacctg acggagcaga accaccacga gatcacgcgc gaacggatgg
     5641 gtgatcaggt cgagcggcag gtaccgcgag acaagctcga acaccgccgg atcccgctca
     5701 tgctcgatca ggcattcaca gagcagcgtc tccagacgcg aaggcggcgc aaaggcggga
     5761 tcgatatccg gatccgcatt gtactccgga gagggtcccg aacccgcgca ctgttcatca
     5821 cgcctttcgc ccgagggatc ggccgcgccc ccatcggtct tgaaagtctg ggccagcgcc
     5881 gctttccggc tcaacacctc gagctgtttc tcgagatcct tctccagtgc ggaatacggc
     5941 aggtgcagcc gttcccccgc ctcttgcaac agcagcgtgc gcaacacgcc accggggcat
     6001 gtggccagag tgccgatcac gccgcgcgca acacggctga ccgcatcgat cgcctcggga
     6061 ttcggctcgt cctgcatctg cgtttcaatc tggaaggcga taagcgaaac cgcgcgttcc
     6121 agcagatccc ggaacggttc ggggccctga tcgcgcaaga tcgaatcggg gtcctccccc
     6181 actggcagac aggccacgcg gaccgggatc tcctcttcca gaaaaagcgt tccggtgtgc
     6241 agcgcggcct tacgccccgc agcatcgccg tcaaagacca gggtcacgct gtcggcatac
     6301 cgtttgagca gtttcacatg gtcgcgggtg aacgccgtac cctgcgacgc gacagcggtt
     6361 tcgaaaccgg aggcgtggca gcggatcacg tcgatctggc cctcgcacac gatcgcctcg
     6421 cgccggggat gtttgacgat ttttccgctg gccttatgga gcgcgtagag aacgcggctt
     6481 ttggtgaaga tcggcgtctc cgggctattg acgtactttc cgacccgctc ccttggcgtc
     6541 aggagtcgtc cgctgaaagc caccacccga ccctgacgat cccaaatcgg gaacatcagc
     6601 cgcccgtgga aacgatcgaa ataacggccc ggaccgccgc ggtccgactc gcagagaatc
     6661 ccggcccgaa ccaacacttc cggagggaag cggttttttt cgagcgcgtt caagagagcc
     6721 tcgacggcat gttccggcgc gtatccgatc ccgaaggact tcacgatctc gtcggacagt
     6781 ttacgctgtg ccagataacg gcgcgcgtgt tccgcctccg gcaggttcaa cagacactgc
     6841 cggtaatact ccgccacggc ggcatgcgcc gcgtacagaa ccgcgcgttc gcccgaggat
     6901 ccctgatcgt ctctgtcctg aagcacaacc ccgacgcgct ccgccatcgc cttgaccgca
     6961 tcggcaaagg tcagaccatc ctgtttcatc acaaacgaga acacatcgcc cttttccccg
     7021 cacccgaaac agtagaagct ctgccgcgtg gggttgaccg tgaacgatgg cgttttctca
     7081 tggtgaaaag ggcaacaggc cttgaaggat gcccccgcct ttttcagcgg cacacgggat
     7141 ccgatcacgt caacaatgtc gacccgggca cggatctctt ctacaacgga ctctgtgatt
     7201 ctaactgcca tgaaccccct tcaccagcct ccccgttgcc ggcacccgtt tgttcgggcg
     7261 gaaccggcgg ggaacggacg ggatatggga agaacatacc atgcccttcc ctcgtctaca
     7321 agccggagcg ataccacccc atctgtttca gcctgtcttc tgcggaacag gcccagacga
     7381 cgggacacct tgatccacag ataacgcaga ttacacagaa gaggatgatc gcaccacaaa
     7441 gggcgcaaag tgcataaaga ctgatttgac cggagcggcc tgtaacctgt aacgatcccg
     7501 gcgcattgac cggacaagcc gcatctggca caatgagcac cgtatatgga cggggcggac
     7561 atcccggccg cacacgaaac gccggatggt cacgggtgaa gaacgacgcc atagaacaac
     7621 gcggtttcca attgcttgaa caactggatg agaccagccg gacccaggtt tggcgcgccg
     7681 tgcaaaccaa tctggaccgg accgtcatcc tgcagatcct gaaagagcat gcggcccaaa
     7741 accccgggga aacggcgcat ttcctgatga tcgcccgccg tatcgcccgc atcaaaagcg
     7801 aggcggtctg tgcccttttc gatattgtca gcgaagcgcc gttgcactat gtggtcatgg
     7861 agcaggtcga cggacccact ctggaagaac tcgttttaca gcatggcccc cttccgttcc
     7921 cgcagattct ccgaatcgcc gcgtccctcg ctgacggtct ggatcagatg tggcgttccg
     7981 cgcatctcgt ccaccggaac ctgaagggtg cgacaatccg cctcgatgag cgcggcatcg
     8041 cgaaaatcac ggatttcagc ctagccattg aggccggtcc cggcatcgac gcaacagata
     8101 tggaccaggg cctgattgtc gggacgccct gttttctttc gccggagcaa gcctccggat
     8161 cccgtacgct gaccacacag tccgacatgt acgcgctggg cgcgctgctt tattatctgg
     8221 cgaccggaca tgtgccgttc gaggagttgg atgtggcgtc gattctcgcg gcgcactgta
     8281 acggtcggat accgcctccg catctcttac gcaacgatct cccgaaagtt ttctcctggt
     8341 ttctgcaccg gctgatgatg aaatcgccgg aaaaccgcta cgccgggtgg gatgcggtcc
     8401 ggcaggatct ggaccggctt cggaaaggca cttcccccgc atcctgcgtt cgtccggatg
     8461 acacggcgcc cagcacagtg gacgccgcac cgttgcgcga agccgtttcc gacacccccg
     8521 cagcggagca agaagcggcg ggtccgcgaa tccggatggc gggccggccg aaaaatgaaa
     8581 agatcacggc gtatcagtcg aaaaccatcg aggaggacca cgcgcgtgag atccggaggg
     8641 aaacccttgt tcgggaaggt ttctgctggc tggttctggc ggtctggttg atagggttgt
     8701 tctgggtgcg cgctgttctg gaacccgcgg aagggcaacc cgacgcgcgg ccggcaccgg
     8761 agcccccgcc gatagacgaa acacaggccg gagacaaaac acctgaagcg gaaaagggga
     8821 aggcaagcac ggacgtcccc gagacttccg atgtccccgt tccgccgccg tcctccgcca
     8881 ccggcaccgc gacggatttg cctgcggtcg gggagatgca tcccgccttg aagaccgcgc
     8941 tgtctcaggc cctgatcaag ggcaacctca tccgcgcccg cgcgctggtg cagtcacatc
     9001 ccgacccctt ccgcaatcag gccgaactgg ccgcgtttct cacggacatg ccggaccccg
     9061 acgcccttgt cgccgaatat ctgctcgatc agaccggcaa accgctacag tgggaacgta
     9121 acggtaaaac gcgcacggtg atcccgcgcg ccatcgtcgg cagcacggcg catctggatg
     9181 ccaacggaaa ccggattgcg gtgccgctgg acacgctgag tccggaggaa aaactgcgct
     9241 ggatgaaccc ggcccgtact gcgcaccaga ccgccgcgca atgcctgttg ctgctgcact
     9301 ctccgcgccg ccatgaaatc cgttcgaaga tggcgcactg cccgcttctg gcgccgtttc
     9361 tggacgaggc gttacgcttg atgcccgaag gcgtcacgcc tcccgctgaa tagacgcctt
     9421 gttcttcagc tcgtcgatcc gcgcatacac cgcctcttcc gcagccaccc gatacaaaga
     9481 ctggatcacg gtttcccgaa cctcctcaaa cgacagggcg cggggcaaca ggcgttccgt
     9541 cactttgaag agatgaagcg ttccgtttcc gaaatcgatc acatcgctcc attgtccggg
     9601 ttgcagcgcg aacagatccc gttcaatcgc ctccggcaac tcgccacgcg tgaccacggc
     9661 gaacgcgccg ccgtcacggt tgtaggtgtc ggaatagtac ggcaccgcct ccccccgcca
     9721 atccaccctc cctgcctcca cgtcactctt gagttgcagc agtgacatgt aaaccgaggg
     9781 gcggctggcc agatcgacaa accggcacac atgttccaga cgcacccctt ccgccacctt
     9841 gaaaagttcg gcgtgcgtgt cgaaatagtt ccgggcggcc gcctcatcgg gcacgggcac
     9901 atccttgcgg atctgtttca gataccgcct gacctgcaac cccccgcgcg cgcgcgccgc
     9961 ccgctccgct tccggatacg gctgataggt ttcggcgtac tgcgtcagct cttgacggat
    10021 gcgcgcttcg gaggggatcg gctgtgtcgc gcacgcctcc tgccgcacca gctcggactc
    10081 gatcacccct tccaccgccc actcccgcag ctgcgcctca tccggctcgc cgccgttcgc
    10141 gcggacatac tcttcatacg ggtgacgcaa acgctcggcc tcgcgggtca cgacctcttc
    10201 agggatctcg attccgttta caaccagcct cattcgcggg gtcctttctt cctgcgttac
    10261 ttcaacaatc cgctggcttt cgggatcgcc atcgagatcc acgggacaaa ggtgatcagc
    10321 atcagggcga taatcatcgc ggcaaaaaag ggcagggctt ttaccgtcac cttggcgatg
    10381 gtcgtcttgc ccacgccgca gccgatgaag aggcaggtgc ccaccggcgg cgtgcagaga
    10441 ccgatgcaga ggttggcgat caggatgatg ccgaagtgga tatccgacaa cccgagctgg
    10501 cgcaccaccg gcagcaggat cggcgtgaag atcaggatgg cgggcgtcat gtccatgaac
    10561 atcccgacca ccagcagcag cgcgttgatc gtgagcagga tgacgaccgg gttttccgag
    10621 aggcccatca gcgcggacga gacggactgc gggatgttgg ccacggtcat gatccacgac
    10681 atcgcgctgg atgatccgat cagcaacatc acaacggctg tggtgatgcc ggtttgcagg
    10741 cagatacccg gcagatcctt ccaggggatc tcgcgataga tcaccacgca caacacgaag
    10801 gcgtaggcga ccgagacggc cgcggcttcc gtggccgtga agatgcccag caggataccg
    10861 ccgatgatca gaatcatcat cagcaggctg gggatggcac gccagcagac aattagcgga
    10921 ttctgccgcg gctcggcttc cgcgtcggga accgccttgc cgtatccgtg acggatcgcg
    10981 atcaccgccg ccgcgatcat aatacagact cccacgagaa taccgggcag gatgccggcc
    11041 atgaacatcg ccgcgatcga caccg
//
LOCUS       DAOSLE010000085        11038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5853_length_11038_cov_8.032505, whole genome shotgun sequence.
ACCESSION   DAOSLE010000085 DAOSLE010000000
VERSION     DAOSLE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11038
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5853_length_11038_cov_8.032505"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..1016)
                     /locus_tag="PK576_06955"
     CDS             complement(159..1016)
                     /locus_tag="PK576_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HOE61369.1"
                     /translation="MGKFRFRKRKHKVRARRGVPVRSMIPNLVTLMAAASGITSIRYS
                     CSGYEGHSRNAVIAIIIACVLDGLDGRVARMLRATSKLGAELDSLADFVSFGVAPALF
                     VYFWIMETVARDSAAYSFRGLFWALALFYAMCCAFRLARFNIMLDGGPTQPHWKPFFM
                     GLPAPGAAGLVLTPAIWHLHTGASFLQSPWIGGGMLLLCGILMASRFPTLSAKNLHVP
                     PRYLMPVLLTVMFLIGMLVSQFWLTLGIIGILYLLSIPVCGAAFRNLLRREHRKTAAA
                     PSPPPTAQD"
     gene            complement(1040..1711)
                     /locus_tag="PK576_06960"
     CDS             complement(1040..1711)
                     /locus_tag="PK576_06960"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOE61370.1"
                     /translation="MSTPDLSCKRFLFPRMHPEGRAWVFAAAGIALLLTWIRLPLGLP
                     GVLLTYGVYLFFRDPERVPPADPRAVVSPADGIVSLITQLPFPIELTEDETPVWRVSV
                     FMSVFNVHVNRMPAAGTVVKTRYVPGAFFNASRDKASTDNERQLYLLRAHSGQLFAVV
                     QIAGLIARRIVRFVNEGQTLERAERFGLIRFGSRVDVYLPPGVTPTVRLGQVMVAGET
                     EIARL"
     gene            1849..2496
                     /locus_tag="PK576_06965"
     CDS             1849..2496
                     /locus_tag="PK576_06965"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOE61371.1"
                     /translation="MKIVVLLGGPGSGKGTIATRLVAADRTFRHVSSGDLLREAVRLQ
                     TDAGREADGYMKRGELVPDELVAAMIREWMGRSGEGASTLLLDGFPRTVRQAEILDET
                     AAGCGAELSAAVLLEVDDATLFDRIAGRRGCPVCGAGYHVTNIPPKREGVCDVCGSAL
                     VTRKDDSFETVRNRLEVYRNQTLPLIDFYRARGVLRRVAGTGPIEGIVDRVRCAI"
     gene            2496..3263
                     /gene="map"
                     /locus_tag="PK576_06970"
     CDS             2496..3263
                     /gene="map"
                     /locus_tag="PK576_06970"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOE61372.1"
                     /translation="MAIPVKNAAELEAMRVSGRMTAEVLRQVAEAVRPGVTTGELDAL
                     TRRLIGEMGAKPSFLGYRGYPGAICISINDEVIHGIPGSRVVQPGDVVSLDVGVYYNG
                     FNGDSATTVMVGVRDPDTIRLVEATRRALAAGVAAVRPGARLSDVSHAVESVARASGC
                     SVVRDFVGHGVGRHLHEEPQIPNYGLPGRGPVLKPGMTLAIEPMVNLGRPEVAVLDDG
                     WTVVTRDGKPSAHFEHTVAVLEEGAEILTRSENFQTL"
     gene            3325..3438
                     /gene="rpmJ"
                     /locus_tag="PK576_06975"
     CDS             3325..3438
                     /gene="rpmJ"
                     /locus_tag="PK576_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOE61373.1"
                     /translation="MKVRSSVRRICERCRVVRRKGVVRIICTNPRHKQRQG"
     gene            3454..3858
                     /gene="rpsM"
                     /locus_tag="PK576_06980"
     CDS             3454..3858
                     /gene="rpsM"
                     /locus_tag="PK576_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007278793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOE61374.1"
                     /translation="MPRLMGVDIPGRKRVEYALRYIHGIGPKRARDVVEFCKIDPAKK
                     ADDLSPDEIRQIVAHIQDKYRVEGDLRREVSQNIRRLISIGTYRGLRHKRGLPVRGQR
                     TRTNARTRKGGKRTVGAVRDKMARAAIKAKSS"
     gene            3936..4448
                     /gene="rpsK"
                     /locus_tag="PK576_06985"
     CDS             3936..4448
                     /gene="rpsK"
                     /locus_tag="PK576_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOE61375.1"
                     /translation="MSEEEAKKEAKKEEEQAAASEQQPEISEAQAVLSEGDDPTKTGT
                     ADVAKKKGKTKSIPAGIAFVRSTFNNTLVSITDARGGVIAWSSAGKAGFKGSRKSTAF
                     AATMVAQDAARQAIAKGMHEVEVRIQGAGAGRESAVRAIQSVGIHITLLKDCTPIPHN
                     GCRPRKRRRV"
     gene            4450..5079
                     /gene="rpsD"
                     /locus_tag="PK576_06990"
     CDS             4450..5079
                     /gene="rpsD"
                     /locus_tag="PK576_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOE61376.1"
                     /translation="MGRYTGPVCRICRREGQKLFLKGARCYMAKCAIEQGKPAPGQHG
                     TRRGRKISGYGEQLRQKQSLRRQFGMQELQFKRFFKEALRRKGVTGEILLQLLETRMD
                     NLVYRLGFASSRRAARQFVLHGHMAVNGKKVAVPSMILKVGDVIEVRDRARSRDLATR
                     SLEAATTQQTPAWLALDRKAFRAELLSIPTREQIAPIVDEQAIVELYSR"
     gene            5145..6140
                     /locus_tag="PK576_06995"
     CDS             5145..6140
                     /locus_tag="PK576_06995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOE61377.1"
                     /translation="MPIRLSHFEMPRRVQKEENTATATYTRFVAEPFEVGYARTIGNS
                     IRRVLLSSIEGVAVTSVKIAGASHEFCALPGISEDVTDIILNLKRLLFKSYSRNPVWI
                     KLHKKGPCVVTAADFAEESAVEVLNPTQYIATLDTDGVLDLEAEIRIGRGFCPAEGNK
                     TPEQEIGRIPIDSVFSPVTRVNFLVENTRVGQRTDYEKLILDVWTDGRVSPEEALKTS
                     AAVLRHHLDVFVDYSEEVLEFEESEKKVDEERDRLRRILNMSVNEIELSVRAANCLNN
                     ANITTVGELAMKTEADMLKYRNFGKKSLNEIKDKLTELGLYLGYKFDPDLFEDNK"
     gene            6160..6570
                     /gene="rplQ"
                     /locus_tag="PK576_07000"
     CDS             6160..6570
                     /gene="rplQ"
                     /locus_tag="PK576_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOE61378.1"
                     /translation="MRHKRLAKKFGRSMSHRKALMSSLVANLVLQDSIKTTLAKAKQA
                     RKDAEKMVTLARKGTLASRRLAAARLRSPSAVQRLFDSVVPAMDGRNGGYTRILKLGQ
                     RRSDGSEMCLLQWVVPPVKKAEAVEADAAAAKKS"
     gene            complement(6594..7064)
                     /locus_tag="PK576_07005"
     CDS             complement(6594..7064)
                     /locus_tag="PK576_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61379.1"
                     /translation="MDKKDSGNFDFRHQAFARFAPATFIALAIPMLSLLPAAYFKRVI
                     PSAAFIPGWDKLIHALLYAALSRALFHALSPKAKERASCWTGVALAAGGYGLLMEFFQ
                     HGLTVSRGFDWFDALANLLGASIAAGLAGWRVRARHRRQGVEKEKDGPHRGPSF"
     gene            complement(7088..7546)
                     /locus_tag="PK576_07010"
     CDS             complement(7088..7546)
                     /locus_tag="PK576_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61380.1"
                     /translation="MTAWRTFPLAAMASALLFAGCATQRELIEKRIGQKHAFFETLPP
                     ETQQRLREGVLMAGDSEDAVWILYGTPDRVFQKVTGTTTNNIWSYVSYDAVPVETARP
                     ILYPVRTARGRVIWRRETFWSPDLFHHPYEYRRIEFRNGRVLSIEAEQQP"
     gene            complement(7543..9762)
                     /locus_tag="PK576_07015"
     CDS             complement(7543..9762)
                     /locus_tag="PK576_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61381.1"
                     /translation="MNTSNTTHTWHFFRSGGLDQALLRTGADLANLKTLDQKLWTALS
                     CPTTGIRFDAATLKLLDTDADGRIRASEILHAIDWLEKRLTSLDPLLIGGDAVKLAAI
                     HTGTAEGKALLSNAKRILKNLGKGDANAITLADVSDIAAIFAETRFNGDGVIPADAAD
                     APALRQTIEEIIACFGAETDRSGKPGINQTKTDAFFTAVAEHLDWVARPAADPAIRML
                     GDQTATAFDALQAVRAKIDDYFTRCRMAEYDPRAAQALSRTDADLAELADKELTDCHA
                     GLADFPLARIEAGRDLPLTAGVNPYWACALASFRDAVVTPLTDGAAETLSLSQWRELK
                     DRFAPFAAWQAACAGTEVASLGADRLEALRNGNDQAEITALIAKDVALEAEIAQITEV
                     EQLIRYHANLAALLNNYVNMGRLYDPEATALFQVGTLFMDARACNLCFEVDHVEAHAA
                     LAAASKCCLVYCSLTRPGAKTRTLCAAFTAGFAQTLWVGRNGIFYDVDGQDWDAVIIK
                     TVDNAISIKEAFWDPWRKIGTMISTQINKLLSARQDAALAAASKNVDAAATAKPAEPP
                     KKMEGAALASSVAALGIAVGLIGSAVGGLVSVLAGLPLWKTALGIMAVVLIVSGPSMI
                     LTWFKLRARDLAPILNACGWAINRRLRFSLKLGRLFTSEAVLPANSSRELMDPYADDS
                     RARNVLLVVLALVAVVAALWFTGLLDSVLPRRLQRQPPAACALRAPQAESPVSAVSP"
     gene            complement(9798..10700)
                     /gene="nadA"
                     /locus_tag="PK576_07020"
     CDS             complement(9798..10700)
                     /gene="nadA"
                     /locus_tag="PK576_07020"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015052948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOE61382.1"
                     /translation="MRIEQEIQTLKAERDAVILAHNYQPGEIQDIADFTGDSLELARE
                     ATHIRARVVVFCGVHFMAETAAILNPDKTVLLPAADAGCPMADMITGEQLRAMKARHP
                     DARVLCYVNSTAEVKAESDCCVTSANAVAVARSFPPQQPLLFVPDRHLGSYTAEQTGH
                     DLILWPGFCPTHARLTEAHIATARAAYPGAPVLVHPECPKPVRDAADAVLSTGGMCQW
                     VKERAEPVFLIGTETGILHRLRRENPDKTFIPLLEQAVCPYMKKTSVEHVLRSLRDLQ
                     TVITVPEPIAQRARRAIDAMLAIH"
BASE COUNT         2200 a         3100 c         3764 g         1974 t
ORIGIN      
        1 atcggggtcg ggatcgggat cgctatcggg atcgattgct tcaccacgtg acaaaaggta
       61 gggcggtttc cccgtaaacc gccgccgggc catgcggacg cctcggggag gcgtccctgc
      121 cgcactttcg gagttcggcg ttcgatgtcc aacattcgtc aatcttgggc agtgggcggg
      181 ggagacggcg cggcagcggt tttcctgtgt tcccggcgca acaagttccg gaaggccgca
      241 ccgcagacag ggatggagag caggtagagg atgccgatga tccccagggt cagccagaac
      301 tgggagacga gcatgccgat caggaacatg acggtcagca gtaccggcat cagatagcgg
      361 ggcggcacgt gcaggttttt ggcggagagg gtcggaaaac ggctggccat gaggatgccg
      421 cagagtagca gcatgcctcc cccgatccac ggggattgca ggaaggacgc tccggtgtgg
      481 agatgccaga tcgcgggcgt caggacgagt cccgcggcgc cgggcgcagg caggcccatg
      541 aaaaacggtt tccaatgcgg ctgggtcgga ccgccgtcca gcatgatgtt gaaccgcgcg
      601 aggcggaagg cgcagcacat ggcgtagaaa agcgccagcg cccagaacag gccgcggaac
      661 gaataggcgg cactgtcgcg cgccacggtc tccatgatcc agaagtagac gaaaagggcc
      721 ggtgccacgc cgaagctcac gaaatcggcg agggaatcca gttccgcgcc gagtttggaa
      781 gtggcgcgca gcatgcgcgc cacccggccg tccaagccat ccagcacgca ggcgatgatg
      841 atcgcgatca cggcgttgcg gctgtgcccc tcatacccgc tgcaactgta acggatcgag
      901 gtgatgccgc tcgccgccgc catcagcgtg acgaggttcg ggatcattga ccggaccggc
      961 acgccacggc gcgcgcggac cttgtgctta cgtttgcgga aacggaattt gcccataatg
     1021 gtatctggaa aaacgtgtgt tagagtcggg cgatttcggt ttcgcccgcg accatcactt
     1081 gcccgagacg gacggtgggc gtcacgccgg gcgggagata cacatccacc cggctgccga
     1141 agcggatcag gccgaaacgc tcggcccgtt ccaatgtctg gccttcgttg acaaaccgga
     1201 cgatgcggcg ggcgatgagc cccgcgatct gcaccacggc aaacagttgg cccgaatgcg
     1261 cgcgcaggag ataaagctgg cgttcgttgt ctgtgctggc cttgtcgcgt gacgcgttga
     1321 aaaaggcacc cggcacgtaa cgggtcttca ccacggttcc ggccgcgggc atccggttga
     1381 catggacgtt gaacacgctc atgaagacgc tgacgcgcca gaccggtgtt tcatcctccg
     1441 tcagctcgat ggggaaaggg agctgcgtga tgaggctgac gatcccgtcc gccgggctga
     1501 ccacggcgcg gggatcagcg ggcggcacac gctccgggtc gcggaaaaag aggtaaaccc
     1561 cgtaggtcag cagcactccc ggcagaccca gcggaaggcg gatccatgtg agcagcaggg
     1621 cgatccctgc ggccgcgaac acccaagcgc ggccctccgg gtgcatccgg gggaaaagaa
     1681 agcgtttaca ggacaaatct ggtgtactca caagagaaaa ccccaatgaa aaaaccagta
     1741 tgtcaggaga gggggggcaa tgcaagaacg gaacgaaaat cggcggcgac cgcgaaagat
     1801 ttcgcgttgc gtcgcgtgcg cggacctgat aaagttacac gcccatacat gaaaatagtc
     1861 gttcttctgg gtggaccggg ttcggggaaa ggcaccatcg ccacgcggct ggtggctgcg
     1921 gaccgcacgt tccgtcatgt ttcgagcgga gatcttttgc gtgaggcggt gcggcttcaa
     1981 acggatgcgg ggcgtgaggc ggacgggtat atgaagcggg gcgagttggt gccggacgaa
     2041 ctggtcgccg cgatgatccg ggaatggatg gggcggtccg gcgagggtgc gagcactctg
     2101 ctgctggacg gattcccccg cacggtgcgg caggccgaga ttttggacga gacggcggct
     2161 ggttgcgggg ccgagctttc cgcggccgtg ttgcttgagg tggacgacgc gaccctgttc
     2221 gaccggattg ccggacggcg cggttgtcca gtatgcgggg cggggtatca cgtgacgaat
     2281 atcccgccca agcgggaagg cgtgtgcgat gtgtgcggga gcgcgctggt gacccggaaa
     2341 gacgacagtt tcgagacggt gcggaaccgg ctggaggtgt atcggaacca gacccttccc
     2401 ctgattgatt tttatcgggc gcgaggcgtg ttgcggcggg tggccggcac ggggccgatt
     2461 gaggggattg tggatcgggt caggtgcgcg atatgatggc gataccggtg aaaaacgctg
     2521 cggagttgga ggcgatgcgc gtgagcggca ggatgaccgc ggaggtcctg cggcaggtgg
     2581 cggaggcggt gcggccgggc gtgacgacgg gtgagttgga tgccctgacc cggcggctca
     2641 tcggggagat gggggccaaa ccctcgtttc tcgggtaccg cgggtatccg ggcgcgattt
     2701 gtatttcgat caacgatgaa gtgatccacg gcattccggg aagccgggtt gtgcaaccgg
     2761 gtgacgtggt gagccttgac gtaggagtgt attacaacgg gttcaacggg gattcggcca
     2821 ccaccgtgat ggtgggggtc cgggatcccg atacgatccg tctggtggag gcgacccgcc
     2881 gcgcattggc ggcgggagtg gccgctgtca ggccgggtgc ccggctctcc gatgtttcgc
     2941 atgcggtgga aagcgtcgcg cgggcatcgg ggtgttcggt ggtccgggat ttcgtcggtc
     3001 acggcgtggg gcgtcatctc cacgaggaac cgcagatccc gaactacggg ttgcccggac
     3061 gcggtcccgt cctgaagccg gggatgacgt tggcgattga gccgatggtc aatctcggca
     3121 ggccggaagt ggccgttttg gatgacgggt ggaccgtggt gacgcgggac gggaaaccgt
     3181 ccgcgcactt cgagcacacc gtggccgtgc tcgaagaagg ggcggagatt ctgacgcggt
     3241 cagagaattt ccaaactttg tgatagacag ggcgacggac ttttggttca atctaaaacc
     3301 tttttttgaa caagggaaaa gaccatgaag gttcgcagtt cagtgcggcg catttgtgag
     3361 cgttgccggg tggtacgtcg caaaggcgtc gttcgcatca tttgtacgaa tccccgccat
     3421 aagcagcgcc aaggttaacg gaagaggaca accatgccga gattgatggg tgtggatatc
     3481 ccgggcagga agcgtgtgga atacgcgctc cggtatattc atggaatcgg tcccaaacgg
     3541 gcccgtgacg tggtcgagtt ttgtaaaatt gacccggcca aaaaagcgga cgatctctcg
     3601 ccggacgaga tccggcagat cgtggcgcac attcaggaca aataccgggt ggaaggcgat
     3661 ctccggcgtg aggtttcgca gaacatccgc cgtctgatca gcattgggac gtaccgcgga
     3721 ttgcgccata agcgcgggct gccggtacgc gggcaacgga cccgcaccaa tgcgcggacc
     3781 cggaagggtg gaaaacggac ggtgggtgcg gtgcgtgaca agatggcacg ggcggccatc
     3841 aaggccaaga gctcctaaca acccggcgtc ggcacggctc tctgctgtta gcgcaggggg
     3901 aaggtgcccg ccacaccatt taaaggctga gacgcatgag cgaagaagaa gcgaagaaag
     3961 aagcgaagaa agaagaagag caggccgctg cttccgagca gcagccggag atcagtgagg
     4021 cgcaggccgt gttatccgag ggggatgacc ccaccaaaac cggtaccgcg gatgtggcta
     4081 agaaaaaagg caagacaaag tcgattcccg cgggcatcgc ctttgtccgg tcgacgttca
     4141 acaacacgct ggtatcgatt acggatgccc gcggcggggt gatcgcctgg agttcggccg
     4201 ggaaggccgg attcaaggga tcgcgcaaga gcaccgcgtt cgcggccacc atggtagcgc
     4261 aggatgccgc ccgtcaggcg atcgccaaag ggatgcacga agtggaagtc aggattcagg
     4321 gcgcaggcgc gggccgcgaa tcggccgtgc gcgcgatcca gtccgtcggc attcacatca
     4381 ccctgctcaa ggattgcacg ccgatccccc ataacgggtg ccgtccgcgt aaacggagga
     4441 gagtctaaca tgggaagata tactggcccc gtttgccgca tttgccggcg cgaggggcaa
     4501 aagctgtttt tgaagggcgc acggtgctac atggcgaagt gcgcgatcga gcagggcaag
     4561 ccggcgcccg gtcagcacgg cacgcgccgc ggccgtaaaa tttccggata cggggagcaa
     4621 ctccgccaga agcagagcct gcgacgccag ttcggcatgc aggaactgca gttcaagcgg
     4681 ttcttcaaag aggccctgcg ccggaaaggg gtcaccggcg agatcttgct gcagttgttg
     4741 gaaacccgca tggacaatct ggtttaccgg ctcggtttcg cgtcttcgcg ccgcgctgcc
     4801 cgtcagttcg tgctccacgg ccacatggcg gtgaacggca agaaggtcgc ggtcccctcc
     4861 atgatcctca aggtcgggga tgtgattgaa gtccgggacc gcgcacgtag ccgagacctc
     4921 gccacacgca gccttgaggc cgccaccacc cagcagacgc cggcctggct cgcgctggac
     4981 aggaaggcct tccgcgctga gttgttgagt atcccgacgc gcgagcagat cgcgccgatc
     5041 gtggatgagc aggcgattgt cgaattgtac tcgcgttaag cgcagatgta ctgacggccg
     5101 ccgtctccca aagggggggc gggcggtctg aaaggaatgg gaacatgccg attcgtttaa
     5161 gccattttga gatgccccgg cgcgtgcaga aggaagagaa caccgcgacc gcgacctata
     5221 cgcgttttgt cgcggagccg tttgaggtgg gttacgcccg caccatcggc aactcgatcc
     5281 gacgtgtgct gttgtcctct atcgagggcg tggcggtcac ctccgtgaag atcgcgggcg
     5341 cgagtcacga gttctgcgcc ctgcccggca tcagcgagga tgtcacggac atcatcctga
     5401 acctcaagcg gctgttgttc aagagctata gccgtaaccc ggtctggatc aagttgcaca
     5461 agaaggggcc gtgcgtcgtg acggcggcgg attttgccga ggagagtgcg gtggaggtgc
     5521 tcaaccccac tcagtacatc gccacgctgg atacagacgg ggtgctggac ctggaggcgg
     5581 aaatccgtat aggacgcggg ttctgtccgg ccgagggcaa caagacgccg gaacaggaga
     5641 tcgggcgcat tccgatcgat agcgttttct ctccggtcac ccgtgtgaat ttcctcgtgg
     5701 aaaacacccg ggtggggcag cgcacggatt atgagaagct gatcctcgat gtctggaccg
     5761 acgggcgtgt ctcgccggag gaagccctca agacctcggc ggccgtgttg cgccaccacc
     5821 tcgacgtgtt tgtggactat agcgaggaag tgctggagtt cgaggagtcg gagaagaagg
     5881 tcgatgagga gcgtgaccgt ctgcgccgta tccttaacat gagcgtgaac gagatcgagt
     5941 tgagtgtccg cgccgcgaac tgcctgaaca acgcgaacat caccacggtg ggcgagttgg
     6001 cgatgaagac cgaggccgac atgttgaagt accggaactt cggcaagaaa tcgctgaacg
     6061 agatcaagga taaactcacc gagttggggc tctatctggg gtacaagttc gacccggacc
     6121 tgttcgaaga taacaagtaa acgtttgagg ttctgagaga tgcgtcataa aagattagcg
     6181 aaaaagttcg ggcggtccat gtcacatcgt aaggcgctca tgagcagcct tgtggcgaat
     6241 ctggtgctgc aggacagcat caagacgacg ttggccaaag cgaaacaggc ccgtaaggat
     6301 gccgagaaga tggtcacgct ggcccggaaa gggaccttgg cctcacggcg tcttgcggcg
     6361 gcgcgtctgc ggagcccgtc cgccgtgcag aggctgtttg acagcgtcgt gccggcgatg
     6421 gacggtcgga acggtggcta cacgcggatt cttaaactcg gccaacgccg cagtgacggc
     6481 tcggagatgt gcctgttgca gtgggtcgtg cctccggtga agaaggccga ggctgttgaa
     6541 gcggacgctg cggcggcgaa gaaaagctga gccgactgcc tgcctgacgc ggtctaaaag
     6601 gacggtcccc gatgggggcc gtctttttcc ttttcaaccc cctgccgccg atggcgcgcg
     6661 cgtacccgcc agcccgccag ccccgccgct atcgacgcac ccaacagatt ggccagcgcg
     6721 tcgaaccagt cgaatccacg gctcacggtc agcccgtgct ggaaaaactc catgagcagc
     6781 ccgtacccgc ctgcggccag tgccacgccg gtccagcagg aggcccgttc tttggccttt
     6841 ggcgagaggg cgtgaaaaag cgcgcgggac agcgccgcat agagcagggc gtggatcagc
     6901 ttgtcccatc ccggaataaa cgccgcggaa ggtatgacgc gcttgaaata tgcggcggga
     6961 agcaacgaga gcatcgggat cgccagcgcg atgaaagtcg ccggggcaaa acgggcaaag
     7021 gcctgatggc gaaaatcgaa gtttccagaa tcctttttgt ccatgaccat aatcgatcgg
     7081 gatcgtgtta cggctgttgt tcggcttcga tggacagaac gcggccgttc cggaactcga
     7141 tgcgccgata ttcgtaaggg tggtggaaga ggtcggggga ccagaaggtt tcgcgccgcc
     7201 agatgactcg tccgcgcgcg gttcggaccg gatacaggat agggcgcgcg gtctccacgg
     7261 gcacggcgtc gtaggagaca tacgaccaga tgttgttggt ggttgtgccg gtcactttct
     7321 ggaacacgcg gtcgggggtg ccgtagagga tccagaccgc atcctcggaa tcccccgcca
     7381 tgagcacgcc ttcccgcagg cgctgctggg tttcaggcgg cagcgtctcg aaaaacgcat
     7441 gcttctggcc gatgcgtttt tcgatcagct cgcgctgggt ggcgcatccc gcaaacagga
     7501 gggcgcttgc catcgcagcc aacgggaagg ttcgccacgc agtcatgggc tcaccgcaga
     7561 gacgggggat tccgcctgcg gcgcgcggag cgcgcacgcg gcgggcggct ggcgttgcag
     7621 gcggcgcggc aaaacggagt cgagcagccc tgtgaaccag agggccgcca cgaccgcgac
     7681 aagcgccaag accaccagca gcacgttccg cgcacgcgaa tcgtccgcgt acggatccat
     7741 cagttcgcgt gacgaatttg ccggcagcac ggcctcactc gtgaaaaggc ggcccaactt
     7801 gagcgagaag cgcaggcggc ggttgatggc ccacccgcag gcattgagga tcggcgcgag
     7861 atcgcgcgcg cgcagtttga accaagtgag gatcatggaa ggtcccgaga cgatcagcac
     7921 tacggccatg atgccgagcg ccgtcttcca gagcggcaat cccgccagca cactgacgag
     7981 cccgccgaca gcggaaccga tcaggccgac cgcgatgccg agggccgcga ccgacgaggc
     8041 cagggccgcg ccctccatct ttttgggcgg ttcggctggt ttcgcggttg cggcggcgtc
     8101 gacatttttg gatgcggcgg cgagcgccgc gtcctgccgc gccgagagca gcttgttgat
     8161 ctgtgtgctg atcatcgtcc cgatcttgcg ccacggatcc caaaacgctt ctttgatcga
     8221 aatggcgttg tccacggttt tgatgattac agcatcccag tcttgcccat cgacatcgta
     8281 gaagatgccg tttcgtccga cccagagcgt ctgcgcgaag cccgctgtaa aggccgcgca
     8341 gagggtacgg gttttggcgc cgggccgcgt gagggaacag tagacaaggc agcatttgct
     8401 ggccgcagcc agtgcggcgt gggcttccac atgatcgacc tcgaagcaga ggttgcaggc
     8461 gcgggcatcc atgaagagcg tgccgacctg aaacaacgcg gtagcctccg ggtcgtagag
     8521 gcgtcccatg ttgacgtagt tgttcagcaa cgcggctaga ttcgcatggt agcggatcag
     8581 ttgctccacc tcggtgattt gcgcgatttc ggcttccagc gccacatctt tggcgatcag
     8641 cgccgtgatt tcagcctgat cgtttccgtt gcgcagggct tccagccggt ccgcgccaag
     8701 ggaagcgact tcggtgccgg cacaggcggc ctgccaggcc gcgaaggggg cgaaccggtc
     8761 tttcaactcg cgccattgcg agagagaaag cgtttctgcc gcaccatccg tgagcggggt
     8821 cacgaccgca tcacggaacg aggccagcgc gcaggcccaa tacggattga cccctgcggt
     8881 gaggggaaga tcccggccgg cttcgatccg ggccagggga aaatccgcca acccggcgtg
     8941 gcaatccgtg agttccttgt cggccagctc ggccaaatcg gcgtccgtgc ggctgagggc
     9001 ctgcgcggcg cgcggatcat attcggccat gcggcaccgg gtgaagtagt catcgatttt
     9061 ggcgcgtacc gcttggagcg cgtcaaacgc cgtggcggtc tggtcgccca gcatccggat
     9121 cgccgggtca gcggcggggc gggccaccca gtcgaggtgc tccgcaacag cggtgaagaa
     9181 tgcgtcggtt ttcgtctgat tgataccggg cttgccgctg cggtcggtct ccgcgccgaa
     9241 acatgcgatg atctcttcga tcgtctggcg caacgctggc gcgtctgcgg catctgcggg
     9301 aatcacaccg tcgccattga accgggtctc ggcgaagatc gcggcaatgt cgctcacatc
     9361 agccagggtg atggcgttcg catcgccttt cccgagattc ttgagaatac gtttcgcgtt
     9421 ggatagcagg gcctttcctt ccgcggttcc ggtatggatc gcggcgagtt tgacggcgtc
     9481 gccgccaatg agcaacggat cgagggaggt gaggcgtttt tcgagccagt cgatggcgtg
     9541 caggatttcc gacgcgcgaa tccggccgtc cgcatcggtg tccaacaact tcagggtggc
     9601 ggcgtcaaaa cggatgccgg tggtcgggca actcagggcg gtccagagtt tctgatccag
     9661 ggttttcaag ttagcgagat ccgcgccggt acggagaagc gcctggtcaa gaccaccgct
     9721 tctgaagaaa tgccacgtat gcgtcgtgtt ggaggtgttc atgctatctt gtccttgcga
     9781 gccacattga ttggggttca gtgaatggcc agcattgcgt cgatggcgcg tctggcacgt
     9841 tgtgcgatag gttccggaac ggtgatgacc gtctgcaggt cccgcagact cctgagcaca
     9901 tgctctaccg aggttttttt catgtacgga cagacggcct gttcgagcag cggaataaag
     9961 gttttgtccg ggttctcccg ccgcaatcgg tggaggatgc cggtttcggt gccgatgaga
    10021 aagaccggtt cggcccgttc cttcacccat tggcacatgc cgccggtcga cagcacagca
    10081 tcagcggcat cgcggaccgg tttagggcac tccggatgga ccagcaccgg cgcgccggga
    10141 tacgcggccc gcgccgttgc gatatgggcc tcggtcagcc gcgcgtgcgt cggacagaaa
    10201 cccggccaca ggataaggtc gtgtccggtc tgctccgccg tgtacgagcc gagatggcgg
    10261 tccggcacga aaagcagcgg ttgttgcggc gggaaactcc gggccactgc cacggcattg
    10321 gccgatgtga cacagcagtc gctttcggct ttgacctcgg cggtgctgtt gacgtaacag
    10381 agcacccggg cgtccgggtg ccgggccttc atcgcgcgga gctgctcacc ggtgatcatg
    10441 tcggccatag ggcaacccgc gtcggccgca ggcagcagaa cggtcttgtc cggattgagg
    10501 atcgccgcgg tttcggccat gaaatgcaca ccgcagaaaa caaccacgcg ggcgcggata
    10561 tgggtcgctt cgcgcgccaa ctccagcgag tcgcccgtga agtcggcgat gtcctgaatt
    10621 tcgccgggct gatagttgtg tgcgagaatt acggcgtccc gctccgcttt cagcgtttga
    10681 atctcctgtt cgatccgcat gatcaagcca cctcaaatga tttgtccggt attcctttca
    10741 ctacggtacc acaggtttcg ttatgacgga acaaagaaaa ggacgcacgt gtgcgactgg
    10801 ctatcgcaac tcttaaggtt ttggaggcga cctcgcattc gtgcggcaaa caggtaagat
    10861 tgaagcgtct tacacacaca ttgcggatag agggtagcca gatttacact ctgagacaag
    10921 ccggaaaagg caatcggttc gtgacagggg gaagagttat cgtgtacggg tacgggtacg
    10981 tgaacggggt atggccaacg ttcacgttct ccaaatcggt atcggtatcg ctatcggg
//
LOCUS       DAOSLE010000086        11000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5871_length_11000_cov_7.803381, whole genome shotgun sequence.
ACCESSION   DAOSLE010000086 DAOSLE010000000
VERSION     DAOSLE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5871_length_11000_cov_7.803381"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..102
                     /locus_tag="PK576_07025"
     tRNA            26..102
                     /locus_tag="PK576_07025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60..62,aa:Arg,seq:tct)
     gene            339..3968
                     /locus_tag="PK576_07030"
     CDS             339..3968
                     /locus_tag="PK576_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61383.1"
                     /translation="MTMTRKMVAAVVAAVAALALRAEYTDGLLWSFSAKQGELDGTFD
                     ATKFVDAVTEGAATPFTLTFNYDDIAARPYFTNMTVRSGLQGKSFQQTCLYMPQPTNG
                     TPGVDFVTKCACATVPHAKLAQTNVTFFIRYSWDGKYYNQKDFGGQYAYNYTITIYDN
                     ARDYGSSKGWTFYFSGVDSYTTTHPYVLRSLWVPYTANDCWLGWNQAAGSDWIDLAVT
                     LRNTDEGETEFKYYVCRAGETSITTYSHITSGKGGIPLPGSGDGKLFGAHGTTLAGGT
                     TPDAFRGAISKVALWDRALSAEEINFVFRDAGMPLILNNENLVLNEDRRVSYVIINGD
                     CTINGPGKLILSTPQGVTVSNGTATLSCPVEFDSGAEAVRMTVDATSKLVQTGVWSGA
                     TPIKLIGPGSDYAAHWFELRGTNTFTGNLIFDRAKVYLYGGASVGTTDGSTSWTVSKG
                     YNGSLVYFMGGTFAENFSAANGNNFTGWWQSTRFAEDTTNIFTGALNLGNAYYYVAEA
                     GSRTFFDGTVAYNDYTPSVNAGAEITFTTNCSYQGSSFYPGGGGITHIYSQRKGGYGT
                     TFEYRVQSGMTWKMYGTNLINGTVGRGGEIHGTLDLNGFDQFMGACRSAQATGVVTSG
                     TPATIIFDQSNYADVVNANVFAGAVNLKKQGANNFGLSGASTSTGTLQIAEGTVTMAP
                     TGSWAGLIDVADGTTLAFASGRTLTAGTMIAVADGGTIALDAQLVADAGQISVDGQPL
                     AVGKYTSANGFISGDGSVIVSAAMGDPIVVEADMTIDEDVLVPSVTFKGQYRISGTGS
                     ISVRGGGIVIDDGAENKKGYAIDVPLMLDGNIAGSGYLTLMKGGSFLSATEITNVNLI
                     VSGTLDAANGMTFVQTPPANDRAELILSNAVINAGTAQITLATEDRYYALRSLANTTN
                     AVNCGLLHGSSSNGRFLTENGAKTDFNCPVNNLSYFCPSGGGEMWFNGKVSAWKLSAS
                     EGAIHLAAAGNVFSFDGTAVPQYATAVAIGKNHTLFCNIAGALNDRRTAIQPNGTLVL
                     TGDQFVGATIGSGTVTSPTGATYDFEQRMFTSSGEIQVTGPTTNTIVFADSVNVTKRG
                     TLMVYFGGVSTSTGELQVVEGAVGFSDAGCWRTCPTVRVGANGRLVLGGMRRFDWTTD
                     LYLETGAKLEIPGGTGVNFANLYIDGVKQPPGYYKKTNLPAFIEGDGTLCTTGEGFGT
                     VLRLR"
     gene            3973..5985
                     /locus_tag="PK576_07035"
     CDS             3973..5985
                     /locus_tag="PK576_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61384.1"
                     /translation="MKRIFAVVPLAAVMCAGAKTITVDYAGTGEYRTIGEAMKVVTKG
                     DIVLVKPGVYREEVRIPVGGASKDRPTILRSAVRGKAVVKGSEIWKNPWTKHGDGTKN
                     VWTSPFDKSFFPVWTNSPYMRTISIADRDRSRVARPVTNAVVDAETYLPRTIGQIFVD
                     GALLTEVTTIRELEGVPDTWMVSMDGEAIWLHPAENRAEPNACEIEWSVRNRCIGANR
                     RGLADVVVDGFTVEHCANQGPFPQIGAVDVRSGVRWIVENNTVRYAKSAGITCGSETW
                     GDWKNGEPWKDQRFPDMPDADQRLMFTRDCEIRYNAVTDCGVHGIAAWNPGGAKIYCN
                     VIERNNFGDYPEFGWEETAGIKIHGAPAVVANNVIRDNEAYGVWFDTGFTDVRCTGNF
                     IASNRRGGIMFETCVGHALVDNNVIIGNRPDENQYYNGDGVYSHNGSGVTVAHNFIGF
                     NGGAGVRFRTTYGKNGGMPDGVHTSSNRYVNNIFCDNAKGDLMIASENKFCRGTVSDY
                     NLFLRTGGQTSHIGTLSPFCYTYYNQGENSWSNLWQQCCSAGLDMGYGAWMEFGHPME
                     LSVWQKVRGLDLHSKVVRLTQGKAAQIAYRELSALFKFPMELEDVRVPAIDGPLTDYL
                     GAEYPAPGTLVRPGPFQKFKCAGKPGYYQALVPQVLKAAPRNLKGE"
     gene            5985..7367
                     /locus_tag="PK576_07040"
     CDS             5985..7367
                     /locus_tag="PK576_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61385.1"
                     /translation="MRILRTCGTLVAVCFAVMMTEAANRVARFENGMGFVADVEKALK
                     SGADYDAFVFRVKSGDDKDEFSRAVEQIRQARPATSVIAVYPREDAAWRAIIDRLIIA
                     PYCDGPEKENAYVDYIVSVNWGVDRKHPKYLALSERANRRTQRLHDSKWGVFNHFLGH
                     GCRTAEEWNAKVNGFDVKALGERLSACGARFYFITIMQGRRWMLAPNATYDRICGTKP
                     GEACAKRDLVLELSEELERRGIDLYLYYTGDGPYLDPELGPKMGLVSPRIGGVDRRFV
                     ENWAAVLEEYAVRYGKRVKGWWVDGCYEELLKYNDDLLDVYTAAIRKGSGEAIVAYNN
                     GVREYFTAYGRSADFTAGEFNDFYAVPKGRFVNGQQAFALIPLGDWTQGGDGAGWCSP
                     GMKISSEKLVNYVRTVNERGGVVAIDVRINPDSSWDADQFEALKALKPISAKASPSMP
                     GACSDHGGKR"
     gene            7439..9922
                     /locus_tag="PK576_07045"
     CDS             7439..9922
                     /locus_tag="PK576_07045"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61386.1"
                     /translation="MKEVARTFLAAGIVLFGSSVLLGDSAEEALALAQRTFAYVAKSV
                     PAAQLETCRRELESNAAWLASTTNDPPAYRAGVERAIRSLRRRILFLHPDLQFAKLLA
                     VQRGLPYTWDLHMVDQYVGRFSRPGPGLVVLENWKDAPKKTVLLADKLPAGTVLNPDL
                     NWDADRILFAFCDHTAAPPAGFDPSQYGFHADATPNDWVSQADPTHPIYGGKFRPGEE
                     VKLAHRRYFIWECAADGSWLRQITGGPGDKMETRDGRQTVLIEDIDPCYLPDGGFAFS
                     STRTQNFGRCHWGRYVPSFLLYRGDLTPLGAPAGSHNIRPLSLGEANEWEPAVLNDGL
                     IAFTRWDYINRNAVWHQSLWTIKPDGTGVAHLYGNYSERVCVGTEIKPLIGSPVCVAT
                     ASAHHSFTLGSLFLLDPRRGEDGDAPITRLTPECPFPESEGWKLPMTYCNPQPVNDTL
                     FFCARSDEPLAYPKDHPRHHRSSCGATWQAPNSFGIWLVDTLGGRELIYQDPAISTFN
                     PIPLVKRPKPPALASLLPPADKAPPYGVCYVENVYDSRVDLPKGSVKALRVNRLINMP
                     ACRRDTLHQGRDLEIYREYLGEVPVTEDGSAAFRIPAGEPIQLQALDKDGMAILTMRS
                     FIHAQKGEVQGCLGCHEQKSQTGRIASAPPNRTVLDPVKSVDLGYPGAFSYLRSVAPI
                     FEKHCLKCHTGGEGKPPSLAGLEGQRLMVAKKHVSFASSYFETHYSKPYDYFAAVSPL
                     TKRLKAGHGNVKLSPEEWRKLILWMDYNVPEFSHGGGYSWNRLETAGPNPPAHDVQGT
                     CGRGTNCMCRSCWVREGGYNKPRNLTTLR"
     gene            10340..10585
                     /locus_tag="PK576_07050"
     CDS             10340..10585
                     /locus_tag="PK576_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOE61387.1"
                     /translation="MPTIKPVSELRNYNNVLREVTADKPVFLTKNGHGRFAIVDIRAY
                     ERLRTELELMSALAEGEESARKHGWVPMDSAMEELEG"
     gene            10588..10932
                     /locus_tag="PK576_07055"
     CDS             10588..10932
                     /locus_tag="PK576_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE61388.1"
                     /translation="MSDVRFSPQAIADLKEIKRYISEDLFNPQAAADLVALVFEKLGT
                     LASMPQTGARLRTDIPVLKGYRFIQCKNYLVFYRMEGKEIVVIRILYARRDYLGLLEP
                     NKGSTDNGRTNP"
BASE COUNT         2247 a         3094 c         3431 g         2228 t
ORIGIN      
        1 cgttttccgg cctgcttttt cagatgcccc tgtagctcag ttggatagag caacggattt
       61 ctaatccgtg ggtcgcaggt tcaagtcctg ccaggggcgc catttcaaaa cccccgtaaa
      121 aacggtcagg gccacgcgat gatgcactcc ggttggccgg gaaggtgcgt gacgatgcgc
      181 acggccacgt ccggcgtgcc caccgtactg catcgtgcca tatcgcgcgc cctcgcacac
      241 cccctgtcac gaaccgattg ctgcggttga cgtggtttcg tttgtgttca cctagggaga
      301 agtgatagaa tatgcgcacg atttgcgaaa ggacgattat gacaatgacg agaaagatgg
      361 ttgccgccgt tgttgcggcg gttgcggctt tggcacttcg tgccgagtat accgacggcc
      421 ttctgtggtc gttttcggcg aaacagggcg agctggacgg taccttcgac gcgacgaagt
      481 ttgttgacgc ggtgacagag ggagcggcca ctccctttac gctaaccttc aactacgacg
      541 acatcgcggc ccgtccgtac ttcaccaaca tgactgtgcg gagcggcctt cagggcaaga
      601 gtttccagca gacctgcctc tacatgccgc agccgacgaa tggcacgccc ggtgtcgact
      661 tcgtcaccaa gtgcgcgtgc gcaactgtgc cgcacgcgaa gctcgcgcag accaacgtca
      721 cgttcttcat ccgttacagt tgggacggca aatactacaa ccaaaaggac ttcggcgggc
      781 aatacgccta caactacacc attacgattt acgacaacgc gcgcgattac ggttcgagta
      841 aggggtggac gttctatttc agcggcgtgg actcctatac caccacccac ccgtatgttt
      901 tgcgttcgct gtgggttccg tatacagcga acgactgctg gcttggttgg aaccaggctg
      961 ccggttcgga ctggattgat cttgcggtga cgctgcgcaa tactgatgaa ggtgagaccg
     1021 agttcaaata ctatgtctgc cgggctggcg aaacctcgat tacgacctac tcccacatca
     1081 ccagcgggaa gggcggaatc ccgcttccgg gcagcggaga cggcaaactc ttcggcgcgc
     1141 acggaacgac gcttgccggc ggcaccacgc ccgacgcctt ccgcggtgcg atctcaaagg
     1201 tcgctctctg ggaccgtgcg ctttcggcgg aagagatcaa ctttgtgttc cgtgatgcgg
     1261 gcatgccgct catcctgaac aacgagaatc tcgtgctgaa cgaagaccgt cgggtgtcgt
     1321 acgtgatcat caatggcgac tgtacgatca acgggcctgg gaagctcatc ctcagcacgc
     1381 cccagggcgt caccgtgagc aacggtacgg cgacgttgag ctgtccggtg gaattcgata
     1441 gcggcgcgga ggcggtgagg atgacggtgg atgccacctc gaagcttgtt cagaccggtg
     1501 tgtggagcgg tgcgacgccg atcaagctca ttggccccgg cagcgattat gccgcgcatt
     1561 ggttcgagct tcgcggcacg aacaccttca ccggcaacct catcttcgat cgcgcgaagg
     1621 tctatcttta cggcggggca tcggtgggca cgacggatgg cagcacgagc tggaccgtca
     1681 gcaaggggta taatggcagt ctcgtctatt tcatgggcgg cacatttgcc gaaaatttct
     1741 ctgcggcaaa cggcaacaac tttaccggct ggtggcagtc gacgcggttt gcggaagata
     1801 cgacgaatat tttcacgggt gcgctgaatc tcggcaatgc ctattactac gtggccgaag
     1861 ccggttcgcg gacgttcttc gacgggacgg tcgcctacaa cgactatacg ccgtcggtca
     1921 acgctggcgc ggagatcacg ttcaccacga actgttccta tcagggctcg tcattctatc
     1981 cggggggcgg cggcatcacc catatctatt ctcagcgtaa gggaggttac ggaacgacct
     2041 tcgagtacag ggtccagagc gggatgactt ggaagatgta cggcacgaat cttatcaacg
     2101 gcacggtggg acgcggcggc gagatccatg gtacgctcga cctcaacggc ttcgatcagt
     2161 tcatgggggc atgccgttcc gcccaggcca ccggtgttgt cacttcgggc acgccggcca
     2221 cgatcatttt cgaccagtcc aattacgcgg acgtcgtcaa cgccaatgtc ttcgctggcg
     2281 cggtgaacct gaagaagcaa ggcgcgaaca acttcggttt gagcggggcc tcgacgtcca
     2341 ccggtacgtt gcagattgcc gagggaacgg tgaccatggc gccgacaggc agttgggcgg
     2401 ggttgatcga tgtcgccgat gggacgacgc tcgcttttgc cagcggccgc acgcttacgg
     2461 ctggcacgat gatcgccgtt gcggacggtg gcaccattgc gctggatgcg cagcttgtcg
     2521 ccgatgcggg ccagatctct gtggatggcc agccgcttgc cgtgggcaag tacacgtccg
     2581 caaacggttt tatttccggc gacggctcgg tcattgtgag cgcagcgatg ggcgatccga
     2641 tcgtggtcga ggcggacatg acgattgacg aagacgtgct cgtgccgtcc gtcaccttca
     2701 aaggacagta caggatttcc ggtacgggtt ccattagcgt tcggggcggc ggcattgtca
     2761 tcgacgatgg agcggagaac aaaaaggggt acgcgatcga cgtgccgctg atgctcgacg
     2821 gcaacatcgc cggcagcgga tatcttacgt tgatgaaggg cggttccttc ctttcggcga
     2881 cggagatcac caacgtgaac ttgatcgtct cgggaacgct tgacgccgcg aatgggatga
     2941 cgttcgtgca gacgccgccg gcgaacgacc gcgccgaact catcctttcg aacgcggtga
     3001 tcaatgccgg aacggcgcag attactctgg cgacggagga ccgctactat gcgctccgtt
     3061 cgttggcgaa tacaaccaac gccgtcaatt gcggcttgct tcatgggagt tcctctaatg
     3121 gacggtttct caccgagaat ggagcaaaaa cagatttcaa ctgtccggtc aacaaccttt
     3181 cgtatttctg tccgagcggt ggcggcgaga tgtggttcaa cggcaaggtc tcggcctgga
     3241 agttgtctgc ttccgaaggc gccattcatc tagcggctgc agggaatgtc ttctctttcg
     3301 acggtacggc ggtgccgcag tatgcgaccg cggttgccat cggcaagaat cacacgcttt
     3361 tctgcaacat cgcgggggca ctcaacgacc gtcgcacggc gattcagccg aatggcacgc
     3421 tcgtgttgac gggggatcag ttcgttgggg cgacgatcgg ctcgggcacg gtcacttcgc
     3481 cgacgggtgc gacctacgac ttcgagcagc gcatgtttac gagttcaggc gaaattcagg
     3541 ttacggggcc gaccacgaac acgatcgtct tcgccgattc ggtgaacgtg acgaagcggg
     3601 gcacgctcat ggtctatttc ggcggtgttt cgacctccac tggcgaactg caggtggtcg
     3661 agggcgcggt cgggttcagt gacgcgggct gctggaggac gtgtccgacc gtgcgcgtcg
     3721 gcgccaatgg ccgtctcgtt ttggggggga tgcgtcggtt cgactggact accgacctct
     3781 atctcgaaac aggggccaag ctcgagatcc ccggaggaac gggcgtcaat ttcgcgaatc
     3841 tctacatcga tggcgtcaag cagccgccgg gttactacaa gaagaccaac cttccggcgt
     3901 tcatcgaagg cgacggcacg ctctgcacga cgggcgaggg tttcggtacg gtattgaggc
     3961 tcaggtaaaa caatgaaaag aatttttgcg gtggtaccgc tggcggccgt gatgtgcgcc
     4021 ggggcgaaga cgattacggt cgattacgct ggcaccgggg aataccgcac gatcggcgaa
     4081 gcgatgaagg tggtcacgaa gggcgacatc gtgctggtga agcccggggt ctaccgcgag
     4141 gaggtgcgca ttcccgtggg cggcgcgtcg aaggaccgtc cgaccatcct gcgcagcgcc
     4201 gtgcgcggca aggccgtcgt gaagggaagc gaaatctgga agaacccatg gacgaagcac
     4261 ggtgacggga cgaagaacgt ttggacctcg ccgttcgaca agtcgttctt tcccgtgtgg
     4321 accaactcgc cttacatgcg cacgatctcg attgccgatc gtgaccgcag ccgcgtggcg
     4381 cgtcccgtga cgaacgcggt ggtggatgcg gagacctatc tgccgcggac gatcgggcag
     4441 atcttcgtcg acggcgcgct cctgacggaa gtgacgacga tccgggaact cgagggcgtt
     4501 cccgacacct ggatggtgtc gatggacggc gaggcgatct ggttgcatcc ggccgagaac
     4561 cgcgcggagc cgaatgcctg tgagatcgaa tggtctgtgc gtaaccgttg catcggcgcg
     4621 aaccggcgcg gactcgccga cgtcgtggtg gacggtttca cggtggagca ttgcgcgaac
     4681 caggggccgt tcccgcagat cggggcagtc gacgtccggt caggcgtccg ctggattgtc
     4741 gagaacaaca cggtgcgtta cgcgaagtcc gccggcatca cctgtggttc cgagacctgg
     4801 ggcgattgga agaacggtga gccctggaag gatcagcgtt tccctgacat gccggacgcg
     4861 gaccagcgac tcatgttcac tagggactgt gagattcgct acaacgctgt gacggactgc
     4921 ggcgtgcacg ggatcgccgc ttggaatccg ggcggtgcga agatttactg caacgtgatc
     4981 gagcgcaaca acttcgggga ctatcccgag ttcggctggg aggagacagc gggcatcaag
     5041 atccacgggg cgccggccgt ggtcgcgaac aacgtcatcc gcgacaacga ggcgtacggc
     5101 gtctggttcg acaccggatt caccgatgtc cgctgcacgg ggaacttcat cgcgtccaac
     5161 cgccgcggcg gcatcatgtt cgagacgtgt gtcggccacg cgcttgtcga caacaacgtc
     5221 atcatcggca accgtccgga tgaaaaccaa tactacaatg gggatggcgt ttactcgcac
     5281 aacggttccg gcgttacggt cgcccataat tttatcggct tcaatggtgg cgcgggcgtc
     5341 aggttccgga cgacctacgg caagaacggc ggcatgccgg acggggttca cacgtcgtcc
     5401 aatcgctacg tgaacaacat cttctgcgac aatgccaagg gcgatctgat gatcgcgagc
     5461 gagaacaagt tctgtcgtgg cacggtttcg gattacaacc ttttcctgcg taccggtgga
     5521 cagaccagtc acatcggcac cctctcgccg ttctgctaca cctactacaa ccagggcgag
     5581 aacagttgga gcaatctctg gcagcagtgc tgttcggcgg gtctcgacat ggggtacggc
     5641 gcatggatgg aattcggcca tccgatggaa ctttcggtgt ggcagaaggt ccgcggacta
     5701 gaccttcatt cgaaagtggt tcgcctgacc cagggcaagg ccgcgcagat cgcctatcgt
     5761 gagctttcgg cgctctttaa gtttccgatg gaacttgaag acgttcgcgt gccggcgatt
     5821 gacggcccgc ttaccgacta tctcggggcg gaatatcccg caccggggac gttggtgcgt
     5881 ccgggaccgt tccagaaatt caagtgcgct ggcaagccgg gttattatca ggcgctggtt
     5941 ccgcaggtgt tgaaagccgc tccgagaaat ctgaaaggag aataatgaga attctaagga
     6001 catgtgggac gctcgttgct gtctgttttg cggtgatgat gacggaggcc gcgaatcggg
     6061 tggcgcggtt cgagaacgga atggggtttg tcgccgatgt cgaaaaggcg cttaagagcg
     6121 gagccgacta cgatgcgttc gtctttcggg tgaagtccgg ggatgacaag gatgagtttt
     6181 caagggcggt ggaacagatt cgccaggcaa ggccagcgac ttcggtgatt gccgtttatc
     6241 ctcgcgagga cgcggcgtgg cgtgcgatca tcgatcggtt gatcatcgcg ccgtactgcg
     6301 atggaccgga gaaggagaac gcctatgtcg actacatcgt gtcggtgaac tggggcgttg
     6361 accggaaaca tccgaaatat ctggcactgt cagaacgcgc caaccgccgt acccaacggc
     6421 ttcacgattc caaatgggga gtcttcaatc attttctcgg tcatgggtgc aggacggcgg
     6481 aggaatggaa cgcaaaggtg aatgggttcg acgtcaaggc gctcggtgag cggctcagtg
     6541 cgtgtggcgc aagattctat ttcatcacga tcatgcaggg ccgacgttgg atgctcgcgc
     6601 cgaatgcgac gtatgaccgc atttgcggaa cgaagcctgg cgaggcatgt gcgaaacgcg
     6661 atcttgtgtt ggaactttcc gaagaactcg aaaggcgtgg catcgacctt tacctttact
     6721 ataccgggga tggaccttat cttgacccgg aactcggccc gaagatggga ctcgtttcgc
     6781 cgcggatcgg tggtgtcgat cggcgatttg tcgagaactg ggcagcggtg cttgaggagt
     6841 atgccgttcg ctacggcaag agggtgaagg gatggtgggt ggatggctgc tatgaggaac
     6901 tgctcaagta caatgacgac ctgcttgacg tgtacacggc ggcgatccgg aagggcagcg
     6961 gggaggcgat tgtggcgtac aacaacggcg tgcgcgagta tttcactgcc tatgggcgtt
     7021 cggcggattt cacggcaggg gaattcaatg atttttacgc agtgccgaaa gggcggttcg
     7081 tcaatggcca gcaggcgttc gccctcattc cgctcgggga ctggactcaa ggcggagacg
     7141 gggccggttg gtgcagtccg gggatgaaga tatcgtctga aaagctggtg aactacgtgc
     7201 gtaccgtcaa cgaacgcggc ggtgtggtcg cgatcgacgt gaggatcaat cccgacagca
     7261 gctgggatgc tgatcagttt gaggcgctta aggcattgaa acccattagc gccaaagcca
     7321 gcccctccat gcccggggcg tgttctgatc atggcggcaa gcgatagggg agggggaggg
     7381 ttgtcagagg gtgccgaggg ggcggaacgc gatggtcttg aaaggagaaa caagaaccat
     7441 gaaagaggtt gcacgcacat ttctcgccgc tggcatcgtc ctgttcggct cttcagtcct
     7501 cttgggcgat tcggcggaag aagcactcgc gcttgcgcaa cgtacgttcg cttatgtggc
     7561 aaagtccgtc ccggctgcgc aactggagac ctgccgacgc gaactggaga gtaatgccgc
     7621 gtggctcgcc tccaccacga acgacccacc cgcgtatcgc gcgggtgtgg agcgggcgat
     7681 tcggtcgctt cgccgccgga ttctctttct ccatcccgac ttgcagttcg cgaagctcct
     7741 cgccgtccag cgcggtctgc cctacacgtg ggacctccac atggtggatc agtacgtggg
     7801 acgcttctcg cgtccaggcc ctggtcttgt ggtgctggag aattggaagg acgcaccgaa
     7861 gaaaaccgtg ttgctcgcag acaagctccc cgcaggcaca gtgctcaacc ccgatctgaa
     7921 ctgggacgcc gaccgcatcc tttttgcgtt ctgtgaccac acagcggcgc cgcccgccgg
     7981 attcgatccg agtcagtacg gcttccatgc ggacgccaca ccgaacgatt gggtgtcgca
     8041 ggccgatccc acgcacccga tctacggcgg aaagttccgc cctggcgagg aggtgaagct
     8101 cgctcaccgc cgttacttca tctgggaatg cgctgcggac ggttcttggt tgcgacagat
     8161 cactggcggc cctggggata agatggaaac acgcgacgga cggcagactg tgctgatcga
     8221 ggacatcgac ccttgctatc tgcccgatgg cggcttcgcc ttctcttcca cgcgcaccca
     8281 gaactttggc cgctgtcact gggggcgcta cgtgccgtcc ttcctccttt accgtggtga
     8341 tctcacacct ctgggcgcac cggctggctc gcacaacatc cgtccactct ctctcggcga
     8401 ggcgaacgaa tgggaaccag ccgtactgaa tgatggcctc atcgctttta cgcgctggga
     8461 ctacatcaac cgtaacgccg tgtggcacca gtcgctctgg acgatcaagc ctgacggcac
     8521 aggcgtggcg catctttacg gtaactattc cgagcgcgtc tgcgtgggga cggagatcaa
     8581 acccctcata ggttcgcccg tgtgtgtggc cacggcctct gcccaccact ccttcacctt
     8641 gggatcgctc ttcctgctcg atccccggcg gggcgaagac ggcgatgcgc cgatcacgcg
     8701 cctcacgccg gagtgcccgt tcccggaatc ggagggatgg aaactgccga tgacatactg
     8761 caatccgcag ccggtgaacg acacgctctt cttctgtgcg cgttcggacg agccgctcgc
     8821 ttatcccaaa gaccacccgc gccaccaccg cagttcctgc ggtgctactt ggcaggcccc
     8881 caactcgttt ggcatctggc ttgtcgatac gctcggcgga cgcgaactca tttatcagga
     8941 tcccgctatc tccacgttca acccgattcc gctcgtgaaa cgtccgaagc cccctgcgct
     9001 cgcctcgcta ttgcccccgg ccgacaaggc gcctccgtac ggcgtctgct acgtggagaa
     9061 cgtgtacgat tcgcgcgtgg atctccccaa aggcagtgtg aaggcgttgc gtgtgaatcg
     9121 tctcatcaac atgccggcct gccgtcgcga tacgcttcac caagggcgcg accttgaaat
     9181 ctacagagag tacctcggtg aagtgcccgt gacagaggac ggttcagcgg cattccgtat
     9241 tcctgcggga gagccgattc aactgcaagc acttgacaag gacggcatgg cgatcctcac
     9301 gatgcgcagt tttattcacg cgcaaaaagg cgaagtgcag gggtgccttg gctgtcacga
     9361 acagaagtcg caaacggggc gcattgcatc tgcgccgccg aaccgcaccg tccttgatcc
     9421 cgtgaagagc gtggatctgg gttatcccgg cgcattcagt tacctgcgta gtgtggcacc
     9481 gatcttcgag aaacactgcc tcaaatgcca cacgggcggc gaaggcaagc cgccatccct
     9541 cgcagggctt gaaggccagc gcctgatggt ggcaaagaag catgtgtcgt ttgcaagttc
     9601 gtacttcgag acgcactatt cgaagcccta cgactatttc gcggcagtga gtccgctcac
     9661 gaagcgtctc aaggctgggc atggcaacgt aaagctttcg cctgaagaat ggcgcaagct
     9721 catcctctgg atggactaca acgtgccgga gttctcacat ggcggcggct acagttggaa
     9781 ccggcttgaa accgctggcc ccaatccgcc cgcgcacgac gtgcagggca cgtgcggacg
     9841 cggcaccaac tgcatgtgcc gaagctgctg ggttcgcgaa ggtggttaca acaagccacg
     9901 taatttgaca actctgcggt aagcgtgtac agcctcccgt acacatctgt tcgccggact
     9961 tgcgggcatc gggaggggca aacgtttgct tgcccgagga cgagcagacg ctcgtccctc
    10021 ccggtttgcg acatcgctat cgggatcgat agagagtgaa gagcggaaaa cgctcaacgt
    10081 caaagttata ggtaagaaat aaaaggtaag aggtaaaaag tcgggaacgc gggcattcgc
    10141 ccgctggcag ggcggtttcc ccgaaaccgc cgcggacgcc tcagggaggc gtccctaccg
    10201 gttgaagaac tttcaccgca ctccaaaatt ttcgcagccc ttgtttgtct catgtcaaac
    10261 cacgaccccc gatactccgc cgttgtattc ctcgaagaat tatgctacgg tccgtttata
    10321 ggaagagagg aggggtgtca tgccgaccat aaagccagtt tccgaactca ggaattacaa
    10381 caacgtttta cgtgaagtga cggcggataa accggttttc ttaacaaaga acgggcacgg
    10441 acgttttgcg atcgttgata ttcgtgcata cgaacggttg cgtaccgaac tggaactcat
    10501 gtcggctctg gcggaaggcg aggagtccgc tcggaaacat ggatgggttc cgatggattc
    10561 cgcaatggag gagcttgagg gctagccatg tccgacgttc ggttctctcc acaggcgatt
    10621 gcggatttga aggagattaa gcggtacatt tccgaagacc tgttcaatcc gcaagccgca
    10681 gcggatcttg tcgcgcttgt ctttgagaag cttggcacac tcgcctccat gccacagacg
    10741 ggtgcacgct tgcgaaccga catccctgtg ctgaagggct accgcttcat ccagtgcaag
    10801 aactatctcg ttttctatcg catggagggg aaggagatcg ttgttatccg tattctgtac
    10861 gcgagaagag actatctggg attgctggaa ccaaacaagg gaagcactga taacggacgg
    10921 accaaccctt aaatttcata ggtctcagtt gccttgccac ggcaacacct tatcacaccg
    10981 cgaaaacgct tcacaaccct
//
LOCUS       DAOSLE010000087        10864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5950_length_10864_cov_6.947174, whole genome shotgun sequence.
ACCESSION   DAOSLE010000087 DAOSLE010000000
VERSION     DAOSLE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10864
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5950_length_10864_cov_6.947174"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..81)
                     /locus_tag="PK576_07060"
     tRNA            complement(5..81)
                     /locus_tag="PK576_07060"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:His,seq:gtg)
     gene            complement(172..1140)
                     /locus_tag="PK576_07065"
     CDS             complement(172..1140)
                     /locus_tag="PK576_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61389.1"
                     /translation="MKRLARMGLLKLCAAAVASAGAQTLGTGTGGLILSDDNRTVLAP
                     PPVHADGELIMAELPLRVMPPRVSFLFGTSGRHGPIDLTDGSRVGNPRAPYVLHLVEG
                     GASFTLSPESATNQVSGPFSTADRTVFTLSGAKVTLVRMPPELRVAVRHAGETGQAPK
                     IGVAPYTPEVTKALYALRTRYVGLANRVDVETADIAFQGVPRVHSRITGNTFTPVVKR
                     SERDKQQTLKGAERSAVTFLDSLFRQAFSLRSQAITDGCVYHFRMPPGEYLLCVMQPV
                     RDPDARGNMGSATAIWWTAFYFDGERSLEVELNGENAITWRDLFKL"
     gene            complement(1214..3814)
                     /locus_tag="PK576_07070"
     CDS             complement(1214..3814)
                     /locus_tag="PK576_07070"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61390.1"
                     /translation="MKGKGIWCWVWGIVGVVWAGTAKLPDLPPQDLPPPAVTPDWLVA
                     MASRHGEDAAREYRLLKREMTTKPMTLSGTVDAAARLAQTFHQQAAILPTDRDPLDVV
                     LRRTRALYEDLAPQVELTAAGARLAELEAEARGVAAVDVPARADCFGRVVALRKTIAF
                     ANPLLKGIDRILFITREALPPNEFDWGVHMCDQYYGFHATVHGTTQGNGLYVLHAPWS
                     ERPRAQNLLAESVIESGARKGQKLGNGGYLSPDLSYDGRQILFAYTDGEPQIRVWNEK
                     TTFHLFRCNADGSGLVQLTDGNVNDFDPCWLPNGRIAFISERRGGYGRCHGRLVPTYT
                     LHSMFEDGSDIVAISVHETNEWQPSVDNNGMIVYTRWDYVDRGFNQAHHPWITYPDGR
                     DARELQGNFHLSERTAPHMVMDLRAIPGSSKYVAIACGHHTEARGSVIILDPTLPDDG
                     AMSQIRRFTPDQLMPEAEYYDWKTTKGSAAYASPWPLSERYMLCVYDGDANAQYGVID
                     YKKRRYAITLLDAFGNKIPLYTHPQISCLSPIPLVPRPRPPVLTHGTQTGRPRLPNGA
                     KPAPIPEAERTSKATVGLINVYNSRYPFPAGTVITALRVWQILPKTEPIVGKPRLGVC
                     DQTPGRQFLGTVPVEKDGSAFFEVPAGVPILFHAVNEKGAAVQGMRSLTYTAPGETLM
                     CAGCHESRTGAAARDTVSSKPVAMRRAPSVLRPGPDGSNPFNYPRLVQPVLDARCVTC
                     HGADRKEGMPDLRKGEVAKNRYRFHTSFVSLVPYVCYYTLMYHGPGWLTMGVQRDSFV
                     EPFTLPGRFGSYASRLHAMLAKGHHNVKLTEEENLRLTLFMDSNAAYFGHDHELERQA
                     LGEIVHPVLQ"
     gene            complement(4034..6538)
                     /gene="hrpB"
                     /locus_tag="PK576_07075"
     CDS             complement(4034..6538)
                     /gene="hrpB"
                     /locus_tag="PK576_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HOE61391.1"
                     /translation="MGVFPVDRILPALADSLAANRTVVLQAPPGSGKTTRVAPFLLDA
                     GAPWLAGRKILLLEPRRLAARAAAAYMARQRGEAIGDTIGYHIRLERRIGPQTRIEIL
                     TEGLLTRRLLDDPELADTGLILFDEFHERSLAADMGLALALDARRALRPDLRLVVMSA
                     TIRPGPVATHLGDADIHEADARCYPVETRLSGHVSTMPLPIRVAEAVIRALHDEPGSV
                     LVFLPGEGEIRRTLERLQGAELPPGVSLYPLYGALSPAEQDAAVAPAPPGRRKVVLAT
                     SIAETSLTIEGIRVVIDSGWMRVPRFSPRHGMTRLETLRVTRDRADQRRGRAGRLEPG
                     VCYRLWDEAADRQISPEALPEILEADLAATVLQAADWGSAAPDGLPWLTPPPDAGWRQ
                     AVGLLHELGALDGPAPDPAAADRVWPAITPRGREMTRRPIHPRLAHMILEAARKGCAR
                     QACLVAAVLSEAPAESALRREIDVRVLLDRLEYAGGSRNRFAFRVRELARQWGHAYPA
                     RDTRELPVGRLLAWAYPERIARRRDAAGRYLLRSGRGAVLEPTESLAQAEWLVAAELQ
                     DGAAEARIRLAAPLTVEEVEEAFDGEFEDRAETYWDARTEAVQAVRRIRLGAITLREG
                     GQAAPDPAALRAALYEGIRQKGVDQLPWTRSARALQARVVFLRQAMPEGGWPDYSDEA
                     LAADLDAWLGPHVEGIMRWNRVQALDLTSILWGALNAHGVSRRRLDEFAPTHIRVPSG
                     SSIPIRYEGDTPYLHVRIQEVFGMTQTPRVAAGRVPVVMSLLSPAQRPVQVTRDLESF
                     WNTGYALVRKDLRGRYPKHYWPEDPREATATRRVRP"
     gene            complement(6535..7914)
                     /locus_tag="PK576_07080"
     CDS             complement(6535..7914)
                     /locus_tag="PK576_07080"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOE61392.1"
                     /translation="MHANTLILIVERGPLVGHRFPVPPNGLLLGRSSRCDLAFLDPSL
                     SRRHCRLEWRGMVLWVVDLNSANQTLVNGQPIHESPLMPNDTITVGETTLRVRQEEVP
                     TVSLSDLPDQDIAVGRVPPPFAAEGGEMLESCALRRRSVRLSLAIGGVLTLLCGAGLL
                     YRLRMDTGAGERIALPTAEFGAATQSDSVPPAPAADPDRSVSSTEADAATTAPKTETE
                     SGRSSSRPAPDQAGDRSDPSASPVRMDNAPQGEKPLPCSLRVESDPPQARVIVNGTDR
                     GETPCVLADPKPGVYTLRVEKSGYETLTRKIMLEGGDAVTERFRLESVTGSLAIRTAP
                     SIVTVMLDGKKVGITRPRGTDASGASEPLVLRDLPEGEHQIEVRRKGYATQRRTAIVK
                     RGEHVAVALALPRQFIPNYEVRTSRSYYRGVLEFIDAEGIRLETAPGVTQTILLRDVK
                     QHGALPAEE"
     gene            8140..>8343
                     /locus_tag="PK576_07085"
     CDS             8140..>8343
                     /locus_tag="PK576_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61393.1"
                     /translation="MSMQVRFALSLLPVFVCVSNTFSAPAFEGAFSGIAPDGSRGIYH
                     GASVGMATLRRDGFASMDGNGTLT"
     assembly_gap    8344..8443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8588..10276
                     /locus_tag="PK576_07090"
     CDS             8588..10276
                     /locus_tag="PK576_07090"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOE61394.1"
                     /translation="MKTSLLLVLGSLSAGGSLLFAQPAPEARFAVMTPDEIRAYERDT
                     LQRLADLSLIPPIINTHPLPKYDYDQLDYGMTLGIARTPGGRLWAAWVAGEDGPKAFM
                     VAATSDDDGETWSKPRFVVDSQAPHLPIPRSVIVGNFWTDPSGRLWFFFDQTMNHYDG
                     RQGVWVTVCTNPDADQPAWSKPKRIWHGAVLNKPTILANGEWWLPIEFPAFSGIGPMR
                     HANQDLEHLRGANVFVSTDQGQTWRRRGCVRFPKPDWDEHMFVELKDGRIWMVARTRK
                     GPMQSFSSDGGKTWTEPDFPSFKHPVARFHIRRLASGRILLVKHGETIDTHEGRSKLT
                     AWLSEDEGQTWKGGLMLDERTGISYPDGFQHPDGTIYISYDRNRSTDGEILLARFTEA
                     DILGRTISSPRSKLKMLISRPLKNRLREMEFNTAAKPLRTEPMAEFKSGKGEIRPLEK
                     GAKIYTNRMYTFHDLPEPLTGRRFVYSGIDSTEAVCSRAGMVYVFTPSTKRNPHDNVV
                     HELFSQGFEKTTVPEFVYTLVDGQARSHETCSVFQKQVKVGETVRFGRWGILVF"
     gene            10384..>10864
                     /locus_tag="PK576_07095"
     CDS             10384..>10864
                     /locus_tag="PK576_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61395.1"
                     /translation="MKKNVVTRRGFMRGAVIAGGAAALGGCSRVQATGKLRKSGEMLR
                     LGVIGAGGQGVTNWSQMLECGNVRVVAMCDTDATMLENAKGWYREKGLKADFNTYADY
                     RKLLESERELDAVLVATPDHTHAPAAIQAMKRGCHVYVEKPLVRTIWEARYFEKVAKE
                     "
BASE COUNT         2056 a         3541 c         3221 g         1946 t
ORIGIN      
        1 aaaatggggc gaaaaacggg actcgaaccc gcgacccgca gaaccacaat ctgccgctct
       61 aaccaactga gctattttcg ccagaaaatg agggtctttt accaaacccg tcctgaaaag
      121 gcaagcccgc aattcgtacg ccttccttcg tcaaacggga aaatccgcat ctcacagctt
      181 gaacaggtca cgccacgtga tcgcgttctc cccgttcagc tccacctcga gcgatctttc
      241 accgtcaaaa taaaacgcgg tccaccagat cgcggtggcc gaacccatgt tcccgcgcgc
      301 gtccggatcg cgtaccggct gcatcacgca cagcaggtat tctcccggcg gcatacggaa
      361 atggtagacg catccatctg tgatcgcctg cgaccgaagg gaaaaggctt ggcggaagag
      421 ggaatccaga aacgtgaccg cactccgctc cgcgcctttg agcgtctgtt gtttgtcacg
      481 ttccgagcgt ttgaccaccg gcgtgaaggt gttgccggtg attcgggagt ggacacgcgg
      541 cacaccctga aacgcgatat ccgctgtttc aacatccacg cggttcgcca gtccgacgta
      601 ccgggttcgt aatgcgtaca gggcttttgt cacctcaggg gtatacggtg ccacgccgat
      661 ttttggtgcc tgcccggtct ctccggcatg ccgcacagcc acgcgcagct cgggaggcat
      721 gcggaccagg gtcaccttcg cgccggacaa ggtgaagacc gtccggtccg ccgtggaaaa
      781 gggaccggac acctgattcg tcgcgctttc cggactcagc gtgaaggacg cgcccccctc
      841 aaccaaatgg agaacatagg gtgccctggg attcccgacc cggctcccgt ccgtcaagtc
      901 aatcggtccg tggcggccgc tcgtgccgaa caggaaagag acgcgcgggg gcataacccg
      961 cagcggcagt tccgccatga tcagttcccc gtccgcatgc actggaggcg gcgccagcac
     1021 tgtccggttg tcatccgaca aaataagccc gccggtgcct gtgcccaacg tctgcgcccc
     1081 agcgcttgcc accgccgccg cgcagagttt caggagcccc atccgggcaa gacgcttcat
     1141 agccccgcct ttcagatttt accgcttcac cttgccttga caccccatcc cggaacgccg
     1201 gtggcctgtc cactcactgc agcaccggat ggacgatctc gccaagagct tgccgttcta
     1261 actcgtgatc atgaccgaaa tacgccgcgt tggagtccat gaaaagcgtt agccggagat
     1321 tctcctcctc cgtgagcttg acgttatgat gacctttggc cagcatcgca tggagccgcg
     1381 acgcgtacga accgaacctt ccgggcaatg tgaagggttc gacaaaggag tcgcgttgca
     1441 cacccatggt gagccacccc ggcccgtggt acattagggt gtaataacag acatacggca
     1501 ccaaactgac gaatgaggtg tggaaccggt accggttttt cgcgacctct ccctttcgta
     1561 aatccggcat cccctccttg cgatccgccc cgtggcacgt gacgcaacgg gcatcgagca
     1621 cgggttgcac cagacgcgga taattgaagg ggttcgaccc gtcgggtccg ggacgcaaca
     1681 cggacggcgc gcgccgcatg gcgaccggtt tggaggacac cgtgtcacgc gccgccgcgc
     1741 cggtgcgcga ctcatgacaa ccggcgcaca tcagggtctc gcccggcgcc gtgtacgtga
     1801 gactgcgcat gccctgaacc gccgcgccct tctcattcac cgcatggaag aggatcggga
     1861 ctccggccgg cacctcgaag aacgcgctgc cgtccttttc gaccggcacc gtgcccagga
     1921 actgacgtcc gggcgtctgg tcgcagaccc ccaggcgggg cttgcccacg atcggttcgg
     1981 tcttgggcag aatctgccag acccgtagag cagtgatcac cgtgcccgct gggaacgggt
     2041 accgcgagtt gtagacgttg atcagtccga cggtggcctt cgatgtccgt tccgcttccg
     2101 gaatcggcgc gggcttcgcg ccgtttggca ggcgcgggcg acccgtttgc gtaccgtgcg
     2161 tcaacacggg cggccggggc cgcggcacga gcgggatcgg gctgaggcag gagatctgcg
     2221 gatgcgtgta gagcgggatc ttgtttccga acgcatccag cagcgtgatc gcgtaacgcc
     2281 gcttcttgta gtcaatcacg ccgtactgcg cgttggcgtc cccgtcgtac acacagagca
     2341 tgtagcgctc gctcaacggc caaggcgacg cgtacgccgc agaccccttg gttgttttcc
     2401 aatcgtaata ttcggcctcg ggcatgagtt ggtccggtgt gaagcgccgg atctgtgaca
     2461 tggccccgtc atccggcaac gtcgggtcta aaatgatcac cgagccgcgc gcctcggtgt
     2521 ggtgtccgca ggcgatggca acgtattttg aggaaccggg gatggcgcgc aggtccatca
     2581 ccatgtgcgg cgcggtacgc tccgaaagat ggaagttccc ctgcaactcg cgcgcgtcgc
     2641 gtccgtccgg ataggtgatc cacggatggt gcgcctgatt gaagccgcgg tcgacatagt
     2701 cccagcgcgt gtagacgatc atcccgttgt tatcgacgct cggctgccat tcgttggtct
     2761 cgtgcacact gatcgccacg atgtcgctac cgtcctcaaa catgctgtgc agcgtgtagg
     2821 tcggcaccag gcgaccgtga caccgcccgt acccgccgcg ccgttcggag ataaaggcga
     2881 tgcggccgtt gggcagccag cagggatcga aatcattcac gtttccatcg gtgagctgga
     2941 ccagccccga accgtccgcg ttgcagcgga aaagatggaa ggtcgtcttt tcgttccaca
     3001 cgcggatctg cggttcgccg tccgtgtacg cgaaaagaat ttgccgcccg tcataggaga
     3061 ggtccggaga aagatatccg ccgttaccca acttctgccc cttgcgcgcg cctgactcga
     3121 tcaccgattc ggcgagcagg ttttgcgccc gcggccgctc cgaccacggc gcgtgcagga
     3181 catagagccc gttgccctgc gtcgtgccgt gcactgtggc gtggaaacca taatactggt
     3241 cgcacatgtg aacaccccag tcgaactcat tcggcggcag cgcctcgcgc gtgatgaaga
     3301 gaatgcggtc gatacccttc aacagcggat tggcgaaagc gatggttttg cgcaaggcga
     3361 ccacccgacc gaaacagtcc gcgcgcgccg ggacgtcgac cgcggccacc ccccgggcct
     3421 cggcctccaa ctccgccagc cgcgcgcccg ctgcggtcaa ctccacctgc ggcgccagat
     3481 cctcatacag ggcgcgtgtg cggcgcagca ccacatccag cgggtcacgg tcagtcggca
     3541 ggatcgccgc ctgttggtgg aacgtctggg caagacgcgc ggcagcatcg accgtgccgg
     3601 agagcgtcat cggctttgtc gtcatctcgc gtttcagcag ccggtactcc cgggccgcat
     3661 cctcaccatg gcgcgacgcc atcgccacga gccagtccgg cgtgacggct ggcggcggaa
     3721 gatcctgcgg cggaaggtcc ggcagtttcg ccgtcccagc ccatacgact cccacgatcc
     3781 cccacaccca gcaccatatc ccttttccct tcatttttct tgcccccgtt cccgatatcc
     3841 attctcttcg ccgaacagat cgctgcggtc atcaacgagg ccatccgcct ccgtcattgc
     3901 gaagattgtg tggttcctgt cacggatcca cccagccatc accaccattg aaccaaactc
     3961 ccgctgatcc gtcgacctcc cggcatgaca aaaatccggt cggctgcatc cgtcatctgt
     4021 gcgcggaacg gcgtcaggga cgtacgcggc gtgtggccgt tgcctcgcgg ggatcttccg
     4081 gccagtagtg tttggggtaa cgcccccgga gatctttacg caccagcgcg tatccggtgt
     4141 tccaaaaact ttcgagatcg cgcgtgacct gcacgggccg ctgggccggt gagagcaggc
     4201 tcatcaccac cggaacacgg cctgccgcca cccgcggggt ttgcgtcatg ccgaacacct
     4261 cttggattcg gacatgcaga taaggcgtgt ccccttcata gcggatcggg atggacgagc
     4321 cgctcggaac ccggatgtgt gtgggcgcga attcgtccag ccgccgtcgc gacacgccgt
     4381 gcgcgttaag ggctccccaa aggatcgacg tcagatcgag tgcctgtacc cggttccacc
     4441 gcatgatccc ctcgacatgg ggccccagcc acgcatccag atccgctgcc agcgcttcat
     4501 cggaataatc gggccagccg ccctccggca tggcctgacg cagaaacacc acgcgggcct
     4561 gcaaggcacg ggcactccgg gtccacggta actgatccac ccctttctgc cggatccctt
     4621 cgtacagtgc cgcacgtagc gcggccggat ccggcgcggc ctggccaccc tcgcgcagcg
     4681 tgatggcgcc cagcctgatc ctgcggaccg cctgaacggc ctcagtgcgc gcatcccaat
     4741 acgtctcggc ccgatcctca aattcaccgt cgaacgcctc ctccacctcc tcaaccgtga
     4801 gcggcgcggc aagacggatc cgagcctctg cagcgccatc ctgaagttcc gcagccacca
     4861 gccattcggc ctgcgccaac gattcggtcg gttccagaac cgcgccgcgt ccgctacgca
     4921 acagatagcg gcccgccgca tcgcggcgac gcgcgatacg ctcaggatac gcccaggcga
     4981 gcaatctccc cactggaagc tcccgcgtat cccgcgcggg ataagcgtgg ccccactggc
     5041 gggccagttc gcgcacccgg aaggcaaagc ggttccgact tccgcccgca tactcgagac
     5101 ggtccaacag cacacgcaca tcaatctccc gacgcagtgc ggattctgcg ggcgcctcgg
     5161 agaggacggc cgcaacgagg caggcctgac gggcacaccc tttccgggcc gcttccagaa
     5221 tcatatgggc aagacgggga tggattggcc gtcgcgtcat ctcgcgtccg cggggcgtga
     5281 tagcgggcca gacgcggtcg gccgcggccg ggtccggcgc aggaccgtcc agcgcaccca
     5341 actcatgcaa caaccccaca gcctgacgcc agcccgcatc cggcggcggc gtgagccacg
     5401 gcagcccgtc cggcgcggcg gaaccccaat ctgcggcctg cagcactgtg gcggcgagat
     5461 cagcctccag gatttccggc agggcttcag gggagatctg gcggtcggcc gcctcatccc
     5521 acagccggta acagactccg ggctccagac gacccgcgcg accgcgcctc tggtcggcgc
     5581 ggtcgcgggt gacgcgcaag gtctcgagcc gggtcatgcc gtgacggggc gaaaaccgcg
     5641 gcacgcgcat ccatcccgaa tcgatcacca cacggatccc ttcaatggtc agcgaggtct
     5701 ccgcgatcga ggtggccagc accaccttgc gccgaccggg cggcgccggt gcgactgccg
     5761 catcctgctc tgcaggcgac aacgcgccgt acaacgggta cagggagacc ccgggcggca
     5821 gttccgcccc ctgtaaacgt tcaagggtgc ggcggatctc gccctcgccg ggcagaaaga
     5881 ccagaacgct gcctggctcg tcatgcaatg cccggatcac cgcttccgcc acccggatcg
     5941 gcaacggcat tgtggacaca tggcccgaca gccgggtttc caccggataa caacgcgcgt
     6001 ccgcctcatg gatgtccgcg tcaccgagat gtgttgccac cggacccgga cggatcgtgg
     6061 cggacatcac caccaaccgc agatcgggac gcagtgcacg gcgcgcgtcg agcgcgaggg
     6121 cgagccccat gtccgccgcc agactccgtt catgaaactc atcgaaaagg atcagtcccg
     6181 tgtcggcgag ttccgggtca tccagcagac gccgggtcag caacccttcg gtcaggatct
     6241 caatacgggt ctgcggcccg atcctgcgtt ccaggcggat atggtaaccg atcgtgtcgc
     6301 cgatcgcctc gccgcgttgg cgggccatgt atgccgccgc agcccgggct gccagacggc
     6361 gcggctcgag cagcaggatt ttgcggcccg caagccaagg ggcacctgcg tccaacagaa
     6421 agggggcgac gcgcgtcgtc ttgccggatc cgggaggcgc ctgaagcact accgtacggt
     6481 tcgcggccag cgagtcagcc aaagcgggaa gaatgcggtc aaccggaaac acccctattc
     6541 ctccgctggc agtgcgccgt gctgtttgac atcccgcagc aaaatggtct gggtgacacc
     6601 cggcgccgtc tccaagcgga tcccctcggc gtcgatgaac tccagcacgc cccggtagta
     6661 cgagcgtgag gtgcggacct cataattggg tatgaactgc cggggcaaag cgagcgccac
     6721 ggcgacgtgt tctccgcgct tcacgatcgc cgtccggcgc tgggtcgcgt accccttgcg
     6781 gcgaacctca atctgatgct caccttcagg cagatcccgc agcaccaacg gctccgacgc
     6841 accggacgca tccgtaccgc gcgggcgggt gatgccgact tttttaccat caagcataac
     6901 ggtcacgatc gagggcgcgg tgcggatcgc aagactgccg gtgacggatt ccagcctgaa
     6961 acgctctgtg accgcgtccc cgccctccaa catgatcttg cgggtcagcg tctcataccc
     7021 gcttttttcc acgcgtaacg tgtacacccc gggtttcgga tcagccagca cgcagggagt
     7081 ttcaccccga tcggtaccgt tgacgatgac gcgcgcctga gggggatcgg actccacgcg
     7141 cagagaacac ggcaaaggct tctcgccctg cggcgcgtta tccatccgca ctggcgaggc
     7201 ggagggatcc gaacggtccc ccgcttgatc cggtgccggt ctggaagaag accgtccgga
     7261 ctctgtttcc gttttcggcg cggttgtcgc cgcatcggct tcggtagacg aaacggatcg
     7321 atccggatcc gcggccggtg cgggaggtac agagtcggat tgcgttgccg cgccgaactc
     7381 tgcggtcggc agcgcgatcc tctcgcccgc gccggtgtcc atgcgcaacc ggtacagcag
     7441 ccccgcgccg caaaggagcg tcagcacacc ccctatcgcc aaggaaagcc gcacggaacg
     7501 ccgccgcagg gcacacgatt caagcatttc cccgccttcc gccgcaaacg ggggcggcac
     7561 cctgcccact gcaatatcct gatccggcag gtcggacaac gacacggtgg gcacctcttc
     7621 ctgccggacg cggagcgtcg tttcgccgac cgtgatcgta tcgttcggca tcaggggcga
     7681 ctcgtggatc ggctgaccgt tgaccagcgt ctggttggca ctgttcagat ccaccaccca
     7741 caacaccatg ccacgccatt cgagccggca gtggcggcgg gaaagcgagg gatcgagaaa
     7801 cgcgagatca caccgactcg accggcccaa cagcaggccg ttcggcggca cggggaaccg
     7861 gtggcccacc aatggccctc tctccacgat cagtatcagg gtgttcgcat gcatcttccg
     7921 tatccgtttc ccttgtcgcc acatcacgtc ctgtccgaca gattcatatt atccggtttt
     7981 acaggtgtct gtcacgcctg aacgagccat tccgtttttg acgcacttta aaacaggcag
     8041 ggggaacatg tagaacacga ggctgatccg tgaaagtacg catggcgagc gatgtgacgt
     8101 gcgattgaca acaggcagtc gttgctgtta aatgaaaata tgtccatgca agtccgtttc
     8161 gcgttgtcgc tgttgcctgt tttcgtatgc gtctcgaaca ctttttcagc ccctgcgttc
     8221 gaaggcgcgt tttccggcat cgcgccggat ggttcgcgcg gcatctatca cggggcgagc
     8281 gtcggcatgg ctaccctgcg ccgcgacggg ttcgcctcca tggacggcaa cggaaccctc
     8341 accnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccccgtt cggttccggt
     8461 tccgcctcac caacgctagc ctgtacgctt tctgggtcag cccgtccgag tctggcgcaa
     8521 gcggcggctg tcttgccgct ggcggccccg gctacaccgg tcacatcgat acccaaggag
     8581 cccttccatg aagacttccc ttctgcttgt cctcggtagc cttagcgcag gaggatccct
     8641 cctgttcgcg caacccgccc ccgaggcgcg ttttgcggtt atgacgcccg acgaaatccg
     8701 cgcctatgag cgcgatacgc ttcagcgcct agccgacctc tcgctcatcc cccccatcat
     8761 caacacccat ccgctgccga agtacgacta cgaccaactc gattacggta tgacgctcgg
     8821 catcgcccgc acccccggtg gacgtctctg ggccgcttgg gtcgctggcg aagacggccc
     8881 caaggctttt atggtcgccg caaccagcga cgacgacggc gaaacctggt ccaaaccgcg
     8941 cttcgtcgtg gacagtcagg caccacacct gccgatcccc cgcagcgtga tcgtcggcaa
     9001 cttctggact gatccgtccg gccgtctctg gttcttcttt gaccagacca tgaaccacta
     9061 cgacggccgc cagggcgtct gggtcacggt ctgcacgaat cccgacgctg accagcccgc
     9121 ctggtcaaaa ccgaaacgga tttggcatgg cgcagtgctc aataagccca cgatcttggc
     9181 taacggcgaa tggtggctcc ccattgaatt tccggctttc agcggcatcg gaccgatgcg
     9241 tcatgccaat caagatctgg agcacctgcg cggcgcgaac gtctttgtct caaccgacca
     9301 ggggcagacg tggcgccgcc gcggttgtgt gcgctttcct aaacctgact gggatgagca
     9361 catgttcgtg gaactcaagg atggccgtat ctggatggtc gcccgtaccc gtaaaggacc
     9421 gatgcagagt ttttcctccg atgggggtaa aacgtggaca gagcctgatt tcccaagttt
     9481 caaacatcct gtcgcgcgtt tccacatccg ccgcctcgca tccggacgca ttctgcttgt
     9541 caaacatgga gagaccatcg atacccacga agggcgctcc aaactgacgg cttggctttc
     9601 tgaggacgag gggcagacgt ggaagggcgg gctgatgctc gacgaacgca ccggcatctc
     9661 ctatccggac ggcttccagc atccggacgg cacaatctac atctcctatg accgcaaccg
     9721 ctccacggac ggagaaatcc tgctcgctcg gtttaccgaa gcggatatcc tcggtcggac
     9781 gatctcctcg ccccgctcga agctgaaaat gctgatcagc cgaccgctca agaaccgtct
     9841 ccgcgaaatg gagttcaata ctgcggccaa gcctttgcgg acagagccga tggcggaatt
     9901 caaatccggc aagggcgaaa tccgcccgct tgaaaagggc gcgaagatct acacgaatcg
     9961 catgtacacc ttccacgatc tccccgaacc gctgacaggc aggcgttttg tttattcggg
    10021 aatcgacagt actgaagccg tctgttcccg ggcaggaatg gtctatgtgt tcacgccgtc
    10081 caccaaacgc aatccgcacg acaatgtcgt ccatgagctg ttctcacaag ggttcgagaa
    10141 aaccactgtt cctgagtttg tctatacgct ggttgacggg caggcgcgtt cccatgagac
    10201 ctgctcggtc ttccagaaac aggtcaaagt tggcgagacc gtccggtttg gccgctgggg
    10261 tatccttgtt ttctaactta aatatgcgca actgtcatct tgaaaaacca agcggtttgg
    10321 cgtactttga aacggattaa aaaatcagtt tacgtcgagc aagccaagag gaggttcgga
    10381 acgatgaaga agaatgttgt aacgcggcgc gggtttatgc gaggggcggt tatagccggg
    10441 ggtgcggcgg ccttgggcgg atgtagcagg gtgcaggcaa ccggcaagtt gcgtaaaagc
    10501 ggcgagatgc tgcgtctggg cgtgatcggg gctggcggac agggcgtgac caactggtcg
    10561 cagatgttag agtgcggtaa cgtgcgtgtg gtggcgatgt gcgacaccga tgcgacgatg
    10621 ttggaaaacg ccaaaggctg gtaccgcgaa aaggggctca aggcggattt caacacctac
    10681 gcggactacc gcaagctgct ggaaagcgag cgggaattgg atgcggtttt ggtggcaacg
    10741 cccgaccata cgcatgcgcc agctgccatt caagcgatga agcgcggctg ccatgtttac
    10801 gtcgagaagc cgctggtgcg caccatctgg gaagcgcgtt actttgagaa ggtcgccaaa
    10861 gagt
//
LOCUS       DAOSLE010000088        10823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_5978_length_10823_cov_8.282132, whole genome shotgun sequence.
ACCESSION   DAOSLE010000088 DAOSLE010000000
VERSION     DAOSLE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5978_length_10823_cov_8.282132"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK576_07100"
     CDS             complement(<1..958)
                     /locus_tag="PK576_07100"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE61396.1"
                     /translation="MNNRSGSMFFAGILTGILIACGLGAFVIRAPRTETGGGGGARTV
                     VLKLAHGLDESHPVHLAMTRMKQRLEELTDGKATMDLYSGGVLGGETDCLEQVQKGEL
                     AMTKVSTAALEAFIPEMKVFSIPFAFRDETHFWNTLHSDIGKKLLAKGAARNFRGLCY
                     YDSGDRNFYSTKKPIRTIDDVKGMKIRVMNSRTAMDMINAMGGSPCPITWGELYTALA
                     QGTVDAAENNPPSFDTSRHFEVCKYFTFSAHQRIPDMVIISTKVWDALPQDVRVALQQ
                     AADESETYQRKLWKEKTGQSLALAKEKGVELITPELDGFRKAC"
     gene            complement(976..2289)
                     /gene="gltX"
                     /locus_tag="PK576_07105"
     CDS             complement(976..2289)
                     /gene="gltX"
                     /locus_tag="PK576_07105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE61397.1"
                     /translation="MSVRVRFAPSPTGNVHIGNIRAAIFNWLFARHEGGRFLLRVEDT
                     DRERSTPEAIRTLLDCMAWLGLDYDEPETYQSQQTERHLALAETLLERGLAYREAKGG
                     GGECVVFRMPKEGRLEFTDLVKGRMRKQAVDTQDFVIVRSNGTPVFHLANVVDDIDMK
                     ITHVIRGDDHVENTFKHIELFRVLGAPVPQYAHLPMIVNQQGKPYSKRDGAAFVGEFR
                     DAGYLPDALFNFLLLLGWAPGDDREVLTREEMVERFTLDRCKSSAARFDLKKLIWMNG
                     EYIRRLPREQFTAEFVRRVEAAGLTTDGTDLPGIIGQMQVRTRFYREIPDSCAYFFTE
                     AYAFDPKAVEKRLRPAGVPELLEEVAAAYAALPEFTPDATHAVLNSLAEARGSGLGAL
                     VHPVRVAVSGLTEGPGLFEMLALLGRDRVCARLRTVATRLRDHQL"
     gene            complement(2370..4124)
                     /locus_tag="PK576_07110"
     CDS             complement(2370..4124)
                     /locus_tag="PK576_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOE61398.1"
                     /translation="MSNLVQLEINGIPVEVPAGTSILKAAQSVQVKIPTLCYHPDLAP
                     WAACGICVVRSSGSPKLLRACCTDVAPGMKITTHDADIVRVRRTVLELILANHPNDCL
                     QCPRNGNCELQRLAADFGIRDIPFPTDTPSIPVDASTPSIVLNPEKCIKCGRCTQVCQ
                     QMQNVWALEFIGRGEKTRMAPAAEVTLNESPCIKCGQCSAHCPVGAIYERDHTRQVWD
                     ALRNPEKICVVQIAPAVRVAIGEAFGLPPGTLMTGETYASLRRLGFKAVFDTNYSADV
                     TIMEEGSEFIKRFKKGKDLPLITSCCPAWTDWMEKYAQDFMSNFSTAKSPQQMMGVLA
                     KTYYAEKMGIDPAKLFMVSVMPCTAKKFEISRSITMSASGYQDIDVVLTTRELARMIK
                     SAGIDFLNLEKEEADSILGEYTGAATIFGVTGGVMEAALRTAYCLITGEAQPPSIEFE
                     AVRGMEGVKEAVIDIKGTPVRIAVAHQMGNVEQVLDAVREDRKAGRKPRYDFIEVMAC
                     RGGCIGGGGQPTGATDSVRKARTAGIYTDDEKCVIRMSHLNPQVQKLYADYLGAPLTE
                     KAHALLHNHYHPKKPYAK"
     gene            complement(4145..5932)
                     /locus_tag="PK576_07115"
     CDS             complement(4145..5932)
                     /locus_tag="PK576_07115"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE61399.1"
                     /translation="MGYKQYILVCGGTACESNQGNALFNALKKAVEKSGLVNEVQVVR
                     TGCLSFCGKGPVVKILPQETFYVEVKPEDAQAIVNEHLVKGRVVSRLLYDKKQSKRNV
                     GIKDIDFYQKQFRVVLRNCGVIDPDKIDEYIARDGYKALERVLFEMKPQDVVDEVSKS
                     GLRGRGGAGFPTGKKWSFAAMQPAGQKYMVCNADEGDPGAYMDRSTLEGDPHSVLEAM
                     TIAGYAIGADKGFIYIRAEYPLAIQRLENAIAQSREYGLLGSNILGSGFSFDIEIRLG
                     AGAFVCGEETALLASIEGRRGMPTPRPPFPAVKGLWGRPTVINNVETLANIPVILLKG
                     SQWFSKIGTATSKGTKVFALTGKINNSGLIEVPMGTTLREIIFDIGGGIRDGKAFKAA
                     QTGGPSGGIIPPQFLDTPIDYENLAKIGSIMGSGGLIVMDEEDCIPDIAKFYLDFTVD
                     ESCGKCAPCRIGGRKLLNYLDKICKGEGSHEDVQNMQEISEAMTRASLCGLGQTASNP
                     VQSSLRYFADEYREHIEEKKCRAGKCRNLLRYSIDTAKCVGCTACARVCPVGAIAGER
                     KQPHVINSETCIKCGHCFEVCKFHAVARA"
     gene            complement(5948..6337)
                     /locus_tag="PK576_07120"
     CDS             complement(5948..6337)
                     /locus_tag="PK576_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014271078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE61400.1"
                     /translation="MAKLTLDGLRKLREQKQAAIAKRDPSNKDGQIIVGMGTCGIAAG
                     AKETFDAIIEELKARGITRVQVRQTGCMGLCHAEPMVEVVAPDMPTVIYGQVDAATGR
                     QIVNEHIINKHLIDNHICDKPAMDIVR"
     gene            complement(6495..6938)
                     /locus_tag="PK576_07125"
     CDS             complement(6495..6938)
                     /locus_tag="PK576_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE61401.1"
                     /translation="MKTFIAEWKDKPGNLIMVLHRVQQDFGYIPREIAFEVAEALEVP
                     LAKIYGVITFYNFFKLKKPGRHPIQVCMGTACYLKGGEDILLELEGILGVGLNTVTPD
                     GEFSVEAVRCLGCCGLAPVMVIDGEVYGKVKTEDLQGILSRIRKG"
     gene            7241..8515
                     /locus_tag="PK576_07130"
     CDS             7241..8515
                     /locus_tag="PK576_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61402.1"
                     /translation="MQASLRRRDFLKRGGALAAFNLVPAHVLRSETAPSNQLTRALLG
                     FGGIARSGNHLGYKATRLVALCDPDAQRVNEGIALAEKNGYGKVFACRDFREILARPD
                     VDIVHICTPPHWHGLMSVMAAQAGKDIWCEKPMTRTIGEGRRVMEAVKANNRIFRLNT
                     WFRFQGGFYGFGTEVKPLKKIMENRLLGWPVKAVVGSVTGFSLKFGWSGMVNVTPQPV
                     PETFDYDLWLGPAPFKPYHRHRTHATFRGYWDYDGGGLGDMGQHYLDPVQYLMEKDDT
                     SPIRIEVDCPPQHPDAVGSFRRITFTYADGCQIILDGDDSLKGAPFLEGPKGKVWPKL
                     KSDIPNLDAKLTELPEPLPQQTDFVEAVKLRRPFALNERNGFRSCTIVNLGLVAMRLG
                     RGFRFDPEKLCAVKDPAADKFIYQQMRAPWVM"
     gene            8545..10551
                     /locus_tag="PK576_07135"
     CDS             8545..10551
                     /locus_tag="PK576_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOE61403.1"
                     /translation="MKTTVLIPLAFVLFAAADLTAQPSAAVWSGQEKEALAGICDDAL
                     ADILRQGAPALEKLFAEVKTGGVSDPLAVTRIAALTQFVMRREGAQSRTAYADALLAA
                     AKRAPEGDVACFFLDQLRWCGLPQQANALREMERSDKPGVAALAAMTVQAVTDDRASK
                     AKPVTPTRCAQLNAEIAALKGKDRTARLFQLFADPDPAIAGVACDWARKDGGRRETER
                     WAAKLDTTTDPLRKTMLLDLLANRNDRRAVGAVAERLCDADNGVALSAHRTLFRLSPS
                     DYAEHLPGVLKTLQPERFVMVRDSARVVPTALLKPALLKAYDGFSDTGKKVAMELFKD
                     RRVSEASRYAIATLNAADAEAAIASYRFLRETAGPDAADTLLQHLLASTGRITPEAQS
                     AYAAAARRDTTGRYLEALTQILKSGTEAQKPLALETAARIGGDTLRQTVENAVADANA
                     DISTAAIRALAEWPEPAALPTLLRLAVNAPDAKRQTLAARGVAKLLNRDGLDKKPYAD
                     LWRTLRGQASEARRKEIDNLFAEEKNVARGKPVTTNVGTQGNNLPAHLVDGTLEKAWH
                     GAKWPAQAQIDLGALHTLRSAHLTFFHDGRRTYTFTLELSEDGKTWKQVADNQAAPKP
                     ARPEGLSLSFAPTPARYVRLNVLKNSVNEAVHVLELKVFEAVFQ"
BASE COUNT         2045 a         3562 c         3116 g         2100 t
ORIGIN      
        1 cgcaggcctt gcggaatccg tcaagctcgg gtgtgatgag ttccacgcct ttctctttgg
       61 cgagcgccag agattgtccg gtcttctctt tccagagctt acgctggtag gtctcagact
      121 cgtccgcagc ctgttgcaat gccacgcgca catcctgcgg aagcgcgtcc cagaccttgg
      181 tgctgatgat caccatatcg gggatgcgct ggtgcgccga gaaggtgaag tacttgcaga
      241 cctcgaagtg gcgcgaggta tcgaaggaag gcgggttgtt ctcggcggcg tcgacggtcc
      301 cctgcgccag cgcggtgtaa agctcgcccc aggtgatcgg gcaggggctg ccgcccatcg
      361 cgttgatcat gtccatggcg gtgcggctgt tcatcacgcg tatcttcatg cctttgacgt
      421 cgtcaatcgt gcgaatgggc ttcttggtgg aatagaagtt gcgatcaccc gaatcataat
      481 agcaaagccc ccggaagttc cgggcagccc cctttgcgag caatttctta ccgatgtcac
      541 tgtgcagggt gttccagaag tgggtctcgt cgcggaacgc gaaggggatg ctgaaaacct
      601 tcatctccgg aataaaggct tccagcgcag cggtggagac tttggtcatg gccagttcac
      661 ccttctgcac ctgctccaga cagtcggtct cgccgcccag tacgccaccg gaatagagat
      721 ccatggtggc cttgccgtcg gtcaactctt ccagacgctg tttcatccgg gtcatcgcca
      781 gatgaaccgg atgcgactca tccaacccgt gcgcgagttt gagcaccacc gtacgggcgc
      841 ctcccccccc tccggtttcc gtgcgcggcg cacgaataac aaaggctccc aatccgcagg
      901 ctatcagaat tccggtcagt atcccggcga aaaacattga accagaccga ttgttcatct
      961 ctcatccctt tcttttcaga gttgatggtc gcgcaaccgg gtcgccacgg tccgcaaccg
     1021 cgcacagact cgatcacggc ccagcaacgc cagcatttca aaaagacccg gcccctccgt
     1081 cagaccggaa accgccacgc gcaccggatg cacgagcgct cccaatccgg acccgcgggc
     1141 ttccgccagc gagttcagca ccgcatgcgt ggcgtccggg gtgaactccg gcagcgccgc
     1201 gtaggcggcc gccacctctt ccaaaagctc cggcacaccc gcaggcctga gacgtttctc
     1261 caccgctttg ggatcgaatg cgtaagcctc tgtgaagaaa tacgcgcagg aatccggaat
     1321 ctcccgataa aaccgggtgc gcacctgcat ctgcccgatg attcccggca gatccgtgcc
     1381 gtctgtcgtg agtcccgctg cttcgacccg ccggacgaat tcagccgtga actgctcccg
     1441 cggcaggcgg cggatgtact cgccgttcat ccaaatcaat ttcttgagat cgaagcgcgc
     1501 ggcgctcgat ttgcaacggt ccagtgtgaa gcgttccacc atctcctcgc gcgtcagcac
     1561 ttcgcggtca tcccccggtg cccagcccag cagcagcaga aagttgaaga gcgcatccgg
     1621 caaatacccg gcgtcacgga attcgcccac aaacgccgcg ccgtcccgct tggaatacgg
     1681 tttgccctgc tggttgacga tcatcggcag atgcgcgtac tgcggcaccg gtgcgccgag
     1741 gacccggaac agctcgatgt gcttgaaggt gttttcgaca tggtcgtcgc cgcggatcac
     1801 atgcgtgatc ttcatgtcga tgtcatccac cacattcgcc agatggaaga ccggcgtgcc
     1861 gttcgaacgc acgatcacga aatcctgagt gtcgacggcc tgtttacgca tccgcccttt
     1921 caccagatcg gtgaactcaa gacgcccctc cttcggcatg cggaaaacca cgcactcgcc
     1981 cccgccgcct ttggcctcac ggtaggccaa cccgcgttcc agcagggttt ccgccaacgc
     2041 gagatgccgc tccgtctgct gactctggta ggtttccggc tcgtcgtagt ccaaccctag
     2101 ccaggccatg cagtcgagca gtgtgcggat cgcctccggc gtggagcgct cacgatcggt
     2161 atcctcgaca cgcagcaaaa aacggccgcc ctcatgccgt gcgaacagcc agttgaaaat
     2221 cgccgcccgg atatttccga tatggacatt cccggtcgga ctgggtgcga aacggactcg
     2281 gacagacatg tgaacctcct tgctcgggac cctccccctc cgctacggaa aggccggggt
     2341 tctccggcct ttccgcatac gcccgccgcc tatttggcgt agggcttctt cggatggtag
     2401 tggttgtgca gcagcgcatg cgccttttcg gtgagcggcg cgcccagata gtcggcatag
     2461 agcttctgca cctgcggatt gagatgggac atgcggatca cgcacttctc gtcatccgtg
     2521 tagatccccg ccgtgcgcgc tttccgcacc gagtccgtcg cgccggtggg ctggccgccg
     2581 ccaccgatgc acccgccacg gcaggccatg acctcgatga agtcataccg cggcttgcgt
     2641 ccggccttcc gatcctcacg caccgcatcg agaacctgct ccacgttccc catctgatgg
     2701 gccaccgcga tccgaacagg ggttcccttg atgtcgatca ccgcttcttt gacgccctcc
     2761 atcccgcgaa ccgcctcgaa ctcgattgac ggcggctggg cttcaccggt gatcaggcag
     2821 tacgccgtac gcagcgccgc ctccatcacg ccgcccgtga cgccgaagat cgtggccgct
     2881 ccggtgtact cgccgagaat cgaatcggcc tcctcttttt cgaggttgag gaagtcgatt
     2941 cccgcgctct tgatcatgcg ggccagttca cgtgtggtga gcaccacgtc aatgtcctga
     3001 tatccgctgg cggacatggt tatactgcgg ctgatctcga atttcttggc cgtgcagggc
     3061 atgaccgaga ccatgaaaag cttcgcggga tcgataccca tcttttccgc ataataggtt
     3121 ttagcgagaa cgcccatcat ctgctgcggc gatttcgcgg tggagaagtt gctcatgaag
     3181 tcctgcgcgt atttctccat ccagtcggtc caggcagggc agcacgatgt gatcagcggc
     3241 aggtccttcc ccttcttgaa ccgcttgatg aattcggagc cctcctccat gatcgtcacg
     3301 tcggcactgt agttggtgtc gaaaaccgcc ttgaagccga gacggcgcag gctcgcgtag
     3361 gtctcgcccg tcatcagcgt acccggcggc aggccgaacg cctcaccgat ggccacccgc
     3421 acggccggcg cgatctgcac cacacagatt ttttccggat tgcggagcgc gtcccaaacc
     3481 tgacgcgtat gatccctctc gtaaatcgcg cccaccgggc aatgcgccga acactggccg
     3541 cacttgatgc agggcgactc attgagcgtg acttccgccg cgggcgccat acgggtcttc
     3601 tcgccgcgcc cgatgaactc gagcgcccag acgttctgca tctgctggca cacctgagta
     3661 cagcgcccgc acttgatgca cttctccggg ttcagcacga tcgacggcgt cgaggcgtcg
     3721 accggaatcg acggcgtgtc ggtcgggaac gggatgtccc ggatgccgaa gtccgccgcc
     3781 agtctctgca attcgcagtt gccgttgcgg ggacattgca gacagtcgtt gggatggttg
     3841 gcgagaatca gctccagcac cgtccgccgg acccgcacga tgtccgcgtc gtgagtggtg
     3901 atcttcatgc ccggcgccac gtccgtacag caggcgcgaa gcaatttggg cgaaccggag
     3961 gatcgcacca cgcagatacc gcacgcggcc cagggcgcga ggtccggatg gtaacagagc
     4021 gtggggatct tgacctgcac ggattgtgcg gccttcagaa tcgaggttcc ggccggaacc
     4081 tcgaccggga tgccgttgat ttcaagttgg acaaggttag acatggatga aaccctttca
     4141 gaaattaagc gcgcgcgacc gcatggaatt tacagacctc aaagcaatgg ccgcacttga
     4201 tgcacgtttc cgaattgatc acatgcggct gtttgcgttc gccagcgatg gcgccaaccg
     4261 gacagacccg ggcgcaggcg gtacacccga cgcatttcgc ggtgtcgatg ctgtaacgca
     4321 gcaggtttct gcatttgccg gcccggcatt tcttttcctc aatgtgctcc cggtattcgt
     4381 cagcaaagta acgcaggctg gactgcaccg gattggaggc cgtctgcccg agaccgcata
     4441 acgaggcccg cgtcatcgcc tcgctgatct cctgcatgtt ttggacatcc tcgtgcgagc
     4501 cctcgccctt gcagatcttg tcgagatagt tcagcagttt gcgtccgccg atccggcagg
     4561 gcgcgcattt gccgcacgac tcatcaaccg tgaagtccag atagaacttg gcgatgtcgg
     4621 ggatacagtc ttcctcgtcc atcacgatca ggccgccgga acccatgatc gatccgattt
     4681 tggccagatt ctcatagtcg atcggggtgt ccagaaactg cggcgggata atcccgcccg
     4741 acgggccgcc ggtctgagcc gccttgaagg ccttgccgtc acggatgccg ccgccgatgt
     4801 cgaagatgat ctcacgcagc gtcgtgccca tcggcacttc gatcagcccg gaattgttga
     4861 tttttccggt cagggcgaag accttggtcc ccttggatgt ggcggttccg atcttgctga
     4921 accactggct acccttcagc aggatgaccg ggatgttagc cagggtctcc acgttgttga
     4981 tgacggtggg ccgaccccac aggcctttga ccgccgggaa gggcgggcgc ggggtgggca
     5041 tgccgcgccg tccttcgatc gacgcgagca gcgccgtctc ttcaccgcac acgaacgcgc
     5101 cggcaccgag acggatctcg atgtcgaacg agaacccgct gccgaggatg ttcgatccga
     5161 gcaaaccgta ttcccgggat tgcgcgatgg cgttttcgag gcgctgaatc gccagcggat
     5221 actcggcccg gatgtagatg aaccctttgt cggcaccgat cgcataaccg gcgatcgtca
     5281 tggcctccaa taccgaatgg gggtcgccct ccagcgtcga acggtccatg tacgcgcccg
     5341 gatcgccctc gtcggcattg cagaccatgt atttctggcc tgcgggctgc atcgccgcaa
     5401 acgaccactt tttgccggtc gggaaaccgg cgccgccgcg tccgcgaagt ccggatttgg
     5461 agacctcgtc cacgacatcc tgcggcttca tttcgaacag gaccctttcg agcgccttgt
     5521 acccgtcgcg ggcgatgtac tcgtcgatct tgtccgggtc gatcacgccg cagttgcgca
     5581 acacgacgcg aaactgcttc tgatagaaat cgatgtcctt gattccgaca ttccgtttgc
     5641 tctgtttctt gtcataaagc agacgcgaga ccacccgccc cttgacaagg tgctcgttga
     5701 cgatcgcctg cgcgtcctca ggcttgacct ccacgtagaa ggtttcctgc ggcagaatct
     5761 tcacgaccgg cccttttcca cagaagctca ggcaaccggt gcgcaccacc tgaacctcgt
     5821 tgacgagacc gctcttttcg accgccttct tcagcgcgtt gaacagcgcg ttgccctggt
     5881 tggactcgca cgctgttccg ccgcaaacca agatgtactg tttgtagccc atgatgtgtt
     5941 tccccgctca gcgaacaatg tccattgccg gcttatcgca aatgtggttg tcgatgagat
     6001 gcttgttgat gatgtgctcg ttcacgattt ggcgtcccgt cgcggcgtcc acctgaccgt
     6061 agatcaccgt cggcatgtcc ggcgcgacca cctcgaccat cggctccgcg tggcagaggc
     6121 ccatgcagcc cgtctgccgg acctgcacac gggtgatgcc gcgggccttc aactcctcga
     6181 taatcgcgtc gaaggtttct ttcgccccgg cggcgatccc gcacgtgccc atcccgacga
     6241 tgatctgccc atccttattc gaaggatcac gcttcgcgat cgccgcctgt ttctgctcac
     6301 gcaatttgcg taaaccatcc aatgtcaact tcgccatgtt ccactccctt gtttcactga
     6361 atcaacgtct gttcctaaat tgacgagaaa agtccgcgcc agcgcgaggg gttccgcgcg
     6421 ttcgaggtca ccggggcctc ccgcaagccg cttttggaat cggcggggag accgtacact
     6481 gacgcgcgca ggactcaccc cttccggatc cgggagagga tcccctgcaa gtcctcggtc
     6541 ttcaccttgc cgtacacctc gccgtcaatg accatcaccg gggccagtcc gcaacagccc
     6601 agacaccgga ccgcctccac cgaaaactcc ccgtccggcg tcacggtgtt cagccccacg
     6661 ccgagaatcc cctccagctc caagaggata tcctcgcccc ctttcagata acaggcggta
     6721 cccatgcaca cctggatcgg atgccgtccg ggtttcttga gcttgaagaa attgtaaaag
     6781 gtgatgacgc cgtagatttt ggccagcggc acttccagcg cctcggccac ttcaaacgcg
     6841 atctcacggg gaatgtatcc gaaatcctgc tgcacacgat gcaacaccat aatcaggttg
     6901 ccgggcttgt ctttccactc ggcgatgaaa gtcttcatct ccggcgtcag cttctgaacg
     6961 gctgccgccg gtttcgatgc ggtactcacg gtctccacct ccacgttcac attgtcggga
     7021 tcccgtaccg tgccgggtcc cgggggattg gttaaaaaag gaatccgggg agtattgcaa
     7081 atttccggat attggccaag caaaaccggt cgttcgaaaa aagcccgccg cacaaaccgg
     7141 tgcttgccaa cccttatccc ttccgatatc ctgattcaat taagtcaatc aagtagtcgg
     7201 catgccggct gcgttgtttt ttcacatcag ggaggggatt atgcaggctt cattacgccg
     7261 ccgtgatttt ctgaaacggg ggggggcgct cgcggcgttc aatcttgtgc cagcccatgt
     7321 tttgcggtcg gaaaccgcac ccagcaacca actgactcgc gcgctgctgg ggttcggcgg
     7381 catcgcccgc tcgggcaacc acctcggata taaggcaacc cgtctggtgg ccctctgcga
     7441 tcccgatgcc caacgggtca acgaggggat cgccctggcc gagaaaaacg ggtacggaaa
     7501 ggtcttcgcc tgccgtgact tccgcgagat tctggcccgt ccggatgtgg atatcgtcca
     7561 catctgcacc ccgccccact ggcacggact gatgtcggtc atggccgccc aggccggcaa
     7621 ggacatctgg tgcgaaaaac cgatgacgcg caccatcggt gagggacgcc gcgtgatgga
     7681 ggccgtcaaa gccaacaacc gtatcttccg cctgaatacg tggttccgtt tccaaggcgg
     7741 cttctacgga ttcggcaccg aagtcaagcc cctcaagaaa atcatggaga accgcctgct
     7801 cggatggccg gtcaaggctg tcgtgggcag cgtgaccgga ttctccctca agttcggatg
     7861 gagcggcatg gtgaacgtca ccccgcaacc ggttccggag acctttgact acgatctctg
     7921 gctggggccc gcaccgttca aaccctatca ccgccaccgc acgcacgcca cattccgcgg
     7981 gtactgggat tacgacggcg gcggcctggg cgacatgggc cagcactacc tcgacccggt
     8041 gcagtatctg atggaaaagg acgacaccag tccgatccgc attgaagtcg actgcccgcc
     8101 ccagcatccg gacgcggtgg gttcgttccg ccgcatcacc ttcacttacg cggacggctg
     8161 ccagatcatc ctggacgggg acgattcact caagggcgcc cctttcctgg agggcccgaa
     8221 aggcaaagtc tggcccaagc tcaaatccga catcccgaat ctggacgcga agctaaccga
     8281 gttgccggaa ccgctgccgc agcagaccga ctttgtcgag gctgtcaaac tccgccgccc
     8341 cttcgcgctg aacgagcgca atgggttccg ctcctgcacg atcgtgaacc tcggcctggt
     8401 ggccatgcgt ctcggccgcg gattccgttt cgatccggaa aaactctgcg cggtcaagga
     8461 tcccgccgcg gacaaattca tctaccagca gatgcgcgcc ccctgggtca tgtgagaccg
     8521 catcccatga aaggattgaa ccgtatgaaa accactgtgc tgattcccct tgctttcgtc
     8581 ctgttcgcgg ccgccgatct gacggcccaa ccctccgcgg ccgtctggtc cggtcaggag
     8641 aaggaggccc ttgccggcat ctgcgacgac gcgctcgccg acatactccg tcagggcgcc
     8701 cccgccctcg agaagttgtt cgccgaagtg aaaaccggcg gggtttccga tcccttggcc
     8761 gtgacccgca tcgccgccct gacgcagttc gtgatgcgcc gggagggtgc gcagtcgcgt
     8821 accgcatacg cggatgcgct gctggccgct gcgaaacgcg cccccgaagg ggatgtggcc
     8881 tgcttcttcc tcgaccagct ccggtggtgc ggcctgccgc aacaggcaaa tgccctccgc
     8941 gaaatggaac gctccgacaa gccgggtgtg gccgcgctcg ccgccatgac cgttcaggcg
     9001 gtcacggatg accgcgccag caaagcgaag cccgtcacgc cgacgcgttg tgcgcaactc
     9061 aacgccgaaa tcgcggcgct gaaagggaaa gaccgcactg cccgcctgtt tcagcttttc
     9121 gccgatccgg atcccgctat cgccggtgtc gcgtgcgatt gggcccggaa ggacggcggt
     9181 cggcgtgaaa ccgaacgctg ggcggcgaaa ctcgacacca ccaccgaccc gctccgcaag
     9241 accatgctgc tcgacctgct tgcaaaccgc aacgaccgcc gggccgtcgg agccgtggcc
     9301 gaacgactgt gcgatgcgga taacggcgtg gcgctctcgg cgcaccgcac gcttttccgc
     9361 ctcagccctt ccgattacgc tgaacacctg cccggcgtgc tcaagaccct gcaacccgaa
     9421 cggttcgtca tggtgcgcga cagcgcccgg gtagtgccga ccgcgctgtt gaagcccgca
     9481 ctgctgaagg cctatgacgg cttcagcgat accggcaaaa aggtcgccat ggaattgttc
     9541 aaggaccggc gcgtgagcga agcgtcccgg tacgcgattg ccacgctgaa tgccgcagat
     9601 gcggaggccg ccatcgccag ttaccgtttc ctgcgtgaaa ccgctggccc ggatgccgcc
     9661 gacaccctgc tccagcatct gctcgcctcg accggccgga tcaccccgga agcccaatcg
     9721 gcttacgccg cagccgcgcg gcgcgatacg accggacgct atctcgaagc cctcacccag
     9781 atcctgaaaa gcggcacgga ggcacagaag ccgctggcgc tggaaacggc cgcgcgcatt
     9841 ggcggcgaca cgctccggca gacggttgaa aacgcggtcg ccgacgccaa cgcggacatc
     9901 tcgaccgccg cgatccgtgc gttggccgag tggcccgaac ccgccgcctt gccgacgctg
     9961 ctgcgtctcg cggtgaacgc gccggatgcc aaacgccaga ctctggcggc gcgcggcgtg
    10021 gccaaactcc tcaaccggga cgggctggac aaaaaaccct atgcggatct ctggcgtacc
    10081 ctgcgcggcc aagcctcgga agcgcggcgg aaggagatcg acaacctgtt cgccgaagaa
    10141 aaaaatgtgg ctcgcggaaa accggtgacc accaacgtgg ggacccaagg caacaatctc
    10201 ccggcccatc tcgtggacgg cacgctggaa aaggcgtggc acggcgcgaa atggcccgcg
    10261 caggcgcaaa tcgatctggg ggccctccat acgctccgat ccgcccacct cactttcttc
    10321 catgacgggc ggcgcaccta cacgttcacg ctggagcttt ccgaagacgg caagacgtgg
    10381 aagcaggttg ccgacaatca ggccgccccc aaaccggccc gccccgaagg cctcagcctc
    10441 tcctttgccc cgaccccggc ccggtacgtg cgtctcaacg tgctgaagaa cagcgtgaat
    10501 gaggcggtcc acgttcttga actcaaggtt tttgaggcgg tgttccaata accctaaaag
    10561 gagcagttga acgggtagaa ttctagcaaa gccccgtaaa cattgggttt tgccgcagtc
    10621 cccgccgcat ctgtttggcg caggacgcgg cggtgcgccc gatgctcttt ttccttagtc
    10681 ccatcccttc gtcgctccac ttcgtcgcaa gcgcaatcga cggagttttg gagaccaatg
    10741 cgtatgcggg actaaggtgt ccgccaaccg gtagggacgc ccgcttctcc cgtcagacct
    10801 tatgtcgccc cgcgacgggc gac
//
LOCUS       DAOSLE010000089        10478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6214_length_10478_cov_7.200518, whole genome shotgun sequence.
ACCESSION   DAOSLE010000089 DAOSLE010000000
VERSION     DAOSLE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6214_length_10478_cov_7.200518"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PK576_07140"
     CDS             <1..536
                     /locus_tag="PK576_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HOE61404.1"
                     /translation="DESRPQGEGAGLGVAISVIAGVLMGAGFFGLVSKGMVKNFACPE
                     TGLMTPYTALVVFAVGLFVSNFLWNTIASLKPVRGEPVPPSSYFTLGSPRLHAVGILG
                     GTIWNIGMALSLIASGKAGTAISYGLGQGATLVAALWGILIWKEFKGAPKVAHQLNAA
                     MFILFVAGLALIIRAGA"
     gene            complement(603..3290)
                     /locus_tag="PK576_07145"
     CDS             complement(603..3290)
                     /locus_tag="PK576_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61405.1"
                     /translation="MNRLFRYGRNGVGQGGSALLIVLGFLSFMMISAVSFAIFMRIER
                     QASSNYRHAITARHMLNAGLVRAIDEVDRHLRDVKFPPWPGRVYPGGTNTLSATVQDT
                     RVLSLESLSFLPGIFVNDVRYQALTGDTRPIWRQLSMPINFNSGENAFGGAEVGRFAF
                     VCVNLSDMLNVNAVSAAQSGTNRINIGHLFRNDGAANTFDNNKQNLDRRYETLQDFYA
                     CMFERNDPTFGSPYHKWLDTGDDLDFGFVSHHVLITDGIVKPEPGNANAFAITENKQP
                     IKNLGSAIPSMDPVFQTALGNALVGGLRNQQVIMAPMIADYLDNDHIPKRFDIPTVEM
                     TPMVSEIRLLQGLTPIIKQELVPDTNPPETIYYLQMTTATDPRARIIEVEVTWPFKHH
                     QERSAIPNYSLEVKAFMKIIPNDGTSLNSASFAGGVAGPQYIPLSQAQDVPIPNAWNT
                     DGGDPNACYKKCIVHLDITQQNLRKNICRSSTKNFQLLDPQFVVNQPFCVALVLFVSV
                     KQGDQYVDIVPHGMSNPNLREPYLESVNKLFFRTAGSHVVVAQGVDLSNGTLGRPLPF
                     VWNGLEVPDPRFNYRVANWVKSGGSMGAVNASTTALLGQDGRDGDIYLSVSDLGRMCS
                     PGELGFIVRPFPHRSGNQDAGVDFDTHTLTDTPDHAHFYRTIRLYDHGGTGENQRRDR
                     IFEYFTEGDPHAVSSEVRVNPLSDLPQVLVAALQNTPVDWFCANEKREGRDFPVFNDT
                     DLMSASTWRAFTNGWLQCVEKVRNNTTITTARDQHLSIHYGDQQYFDWYAGQNPRVIF
                     GQTLSDPLHEVDRKMLFSWSIANFSDRQQLFLYVLRAEATVPSFGGSDMGGVKSVAGG
                     RAVALVWRDPYPVGYPDNMVSDPANFHEHRVLFFKQLDN"
     gene            complement(3347..3799)
                     /locus_tag="PK576_07150"
     CDS             complement(3347..3799)
                     /locus_tag="PK576_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61406.1"
                     /translation="MRKPAETLRASRLPSLGFTLVEMLVVVGMLGVLMAVSFSGVGQA
                     RKQAKIAKANSETRELINAWLAYEAAHDDWPVEISGDEIIADQGALGELLGENEEQAV
                     YLNASLTGGIFLDPWGNPYRLRLLYETRQNPKTDEFPASVTFPNRHRR"
     gene            complement(3829..4632)
                     /locus_tag="PK576_07155"
     CDS             complement(3829..4632)
                     /locus_tag="PK576_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61407.1"
                     /translation="MGTMVNLQIPNLKSRGCRAGTVSENVWPLRAGTPLRGVRGRFGE
                     PSLPTINRSHAGFSMIEVLVAASILVVIVMMLGMLFQQTSLAWRSGSFRAEAYMQVRS
                     YIGAIQRDVSAAVDARLLPQQYVTEEQNFSGGRLVFYTLTGGVELDQNDNLLFNRSLK
                     KITYDTAGNRTEQSLKPDGSWGAVKKANVLTFAERQSSKNRPGVKPLQFTPHFGQSGD
                     GLDSQGLPLHVTFGAQARSTGNTLDIGAWSFGPDGRPNTTDDIKTWVEE"
     gene            complement(4671..5264)
                     /locus_tag="PK576_07160"
     CDS             complement(4671..5264)
                     /locus_tag="PK576_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61408.1"
                     /translation="MRGRMTERCGWNRVERYAVAGGDARDPGSIRPFSVRAGFSLMEV
                     NMAIFVMAVGILSMVALYPLGLRESVQGQQDLTQSMFADYLLNQAVAVASMTNFPWSQ
                     WKSVPSIASLDQSGITASQTDNRPAFMRERMRQADDWPYWPSDRFRIICCRGAGGSDR
                     IMGIMVRSTEIEGEDVRNYTNNPVYYAEAYFQGDVTK"
     gene            complement(5306..6184)
                     /locus_tag="PK576_07165"
     CDS             complement(5306..6184)
                     /locus_tag="PK576_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE61409.1"
                     /translation="MAKSQISNLKSRGFRSAAGFSLLELLSVMAIVALLSTLAVTSYF
                     NAIRGMTRRSAVKHLANSLILARQRACLEGVRVSVMVYNEVAGYNNKGDPECVPSYVV
                     CKEAGRVTALSGTTLVDEFTPLDKMFGTATEDDTVGSSYLGSMRLYNLTAGKWWQVTP
                     TAVSHSFSGRRSSYVANRSYTIPAFGFKKNANVRNANDAMWEVGDVYGIEAAPPGSLP
                     KGFKFTNLDGNSDTLPENLNKVELITFLPDGTAQKAILPDGTAQKAMTVTIRELLPPK
                     LRSEIRVQRDGSIQYK"
     gene            complement(6193..6756)
                     /locus_tag="PK576_07170"
     CDS             complement(6193..6756)
                     /locus_tag="PK576_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE61410.1"
                     /translation="MVKSQISNLKPRSTGFTILELLVVVSIMAVIATLATGAVVKAVK
                     QGRKKRVEATCRALEMALTNYRAQEGKWPFKLSDLKRQGTEGQSRNDKKYWAHGADNA
                     KVFKDLYQGAGQKTVYLDASAVMALLDGQRVQLRAALSAGRRDVPLIYADPNETSILR
                     YYCVEYRVETDTVAVHPHSDHSPCKDK"
     gene            complement(6820..7566)
                     /locus_tag="PK576_07175"
     CDS             complement(6820..7566)
                     /locus_tag="PK576_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE61411.1"
                     /translation="MSETFMWYWNNIRHPVADGHRLGYGGGAGGTPALPTGIRIKHAL
                     HTAPPVSGRLRSGFTLVEMLLVIGILGILATALVGSFSYVQKAARQSQAQSLASELAT
                     ALNIYLQQERSWGDVLPNKSEMDEEACWVLQEKKLFDVTTYKSGGAEINNDSLDRYGL
                     LDPYGRALLRRDPGINAGEVIKHRLQIRLDDDYDGYVEAPDGTKVRASVLVWSRGPDG
                     KDDAEKGGRRYPDDDRLSWNHGKARAEAGK"
     gene            complement(7684..8748)
                     /locus_tag="PK576_07180"
     CDS             complement(7684..8748)
                     /locus_tag="PK576_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE61412.1"
                     /translation="MRNARNSVRKIGVSRGFTLIEMMVVIAVIGILIGGVFKLLSTAG
                     TSSQRAKTIARLERMQNAISGFYAEFGTYPPVPSHGSPDPYMEADKDGTYTGSAELNP
                     TNANRAARCQPVAFCYPNARSLDFYINERYQSEGAVSVNDAFGPDVRNRNDYEWDQIR
                     IFRFGLMSYLMPRVELMLNGSELDNLSENNENIPNAYFFDTRQWTHYNAGSKISGPSG
                     GWWNKLRESLAAQRDRENHAVARWLPNLEKSVCGGPGDLLGINTTEPDTGLSMEPTTD
                     PKSSAVTYMLMRVSVRDGWGEDLYYYSAAPYQSYRLWSAGPDKKTFPPWISLESLSAD
                     DRRIVSEWLKDDIARFDRSK"
     gene            complement(8835..10136)
                     /locus_tag="PK576_07185"
     CDS             complement(8835..10136)
                     /locus_tag="PK576_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE61413.1"
                     /translation="MPKFAYIAVDAAGKESRGTIEAPNQAQAVAKVRSQGLFPTAIGV
                     AGGSDAATARPAAAAKKSPAAKKSLAQTRIEIKLPKFLRGKVKQKDLTTFTRQLATLV
                     DAGLPLLRGLHVLQRQTPNAALKDALAGMCESVESGSTFSESLTNYPKIFDNLYVNMV
                     KAGEAGGVLEVVLNRLAEFAEKAAKIKNKVKSAMVYPVVVLVAAVGITGFLLVAVIPK
                     FKDIFNDLLEGKPLPAITQFVMDASNLVMNNGLAVVAAFAALFVLFKIFAKTRFGRYL
                     LDVSKINAPMFGSLVRRTAIARMTRTLGTLLASGVPVLQALNIVRDTTGNEVISRAMQ
                     NVHDAVKEGESMTNPLAACKVFPPMVISMVEVGEETGALPDMLTRIAETYDDEVDNAV
                     AGLTSVIEPIMIIILAVVVGTIVIAMFMPMIQIIGSLGAQA"
     gene            complement(10286..>10478)
                     /locus_tag="PK576_07190"
     CDS             complement(10286..>10478)
                     /locus_tag="PK576_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61414.1"
                     /translation="GYGGIANIHVLQIGIGIAIGIGIAIGFEIDCCARAVARSDKCND
                     GGDPDSDPDPDGDGAAMEP"
BASE COUNT         2093 a         3222 c         2957 g         2206 t
ORIGIN      
        1 cagacgagag ccgcccgcaa ggggaaggag ccggtctcgg tgtcgcgatt tcagtgattg
       61 ccggcgtgct gatgggggcg gggttcttcg ggttggtttc aaagggtatg gtcaaaaatt
      121 tcgcgtgtcc cgaaacgggt ctgatgacgc cgtacacggc gctggtggtg tttgccgtcg
      181 gtctgtttgt ctcgaatttc ctttggaata caatcgcttc gctcaagccg gtacgggggg
      241 agccggtgcc gccctcgtcc tatttcacgt tgggcagtcc gcggctgcat gcggtcggca
      301 tcctcggcgg cacgatctgg aatatcggca tggcgctgag cctgatcgcg tcgggcaaag
      361 ccggcaccgc catttcgtac ggtctcggtc agggcgccac gctggtggct gccttgtggg
      421 gcatcctgat ctggaaagag tttaagggtg cgccgaaagt tgcgcaccaa ttgaatgccg
      481 ccatgtttat cctgtttgtg gcggggttgg cgctgattat ccgggccggc gcatgatcct
      541 ccacgcacgg gggatgaacg gttctgtttg agaagtccgc tccccctgtg aaagggattc
      601 catcagttgt cgagttgctt gaagaacagg acacggtgct cgtggaaatt cgcggggtca
      661 ctcaccatgt tatcgggata tcccacggga tagggatcac gccagaccag cgccaccgcg
      721 cggccgcctg ccacggactt aaccccgccc atatcgctgc caccgaacga gggcaccgtg
      781 gcctcggccc gtagcacgta gaggaaaagc tgctgccggt ccgagaagtt ggcgatcgac
      841 caagaaaaca gcatcttgcg gtccacctcg tgcagcggat ccgagagcgt ctggccgaaa
      901 atcaccctag ggttctggcc cgcgtaccag tcgaaatact gttgatcccc gtagtggatg
      961 gagaggtgct gatcccgcgc cgtggtgatc gtcgtattat tccggacctt ttcgacacat
     1021 tgcaaccacc cgttcgtgaa cgcccgccaa gtagaagcgg acatcaagtc cgtgtcgttg
     1081 aacaccggaa aatcccggcc ttcccgcttt tcgttggcac agaaccaatc caccggcgtg
     1141 ttttgaagcg cggccaccag cacctgcggc agatccgaga gcggattcac ccgcacttcc
     1201 gatgatacgg catgcggatc cccttcagtg aaatattcga aaatccggtc gcgcctctga
     1261 ttttcccctg ttccgccatg gtcatacagc cggatcgtgc ggtaaaaatg cgcgtggtcc
     1321 ggcgtatccg taagtgtgtg cgtatcgaaa tcgactcccg cgtcctgatt cccgcttcta
     1381 tgcgggaaag gcctgacaat aaaacccagt tcacccggag aacacattct gcccaaatcc
     1441 gaaaccgaga gatagatatc cccgtcacga ccatcctggc ccagcagcgc cgtagtcgaa
     1501 gcattgaccg cccccatgga cccgccactc ttgacccagt tggcaacacg atagttgaaa
     1561 cgggggtcgg gaacttccag cccgttccaa acaaaaggca agggcctgcc caaagttccg
     1621 ttcgacaaat caacaccctg cgccacgacc acatgcgacc ccgccgtgcg gaaaaagagc
     1681 ttgttcacgg actccaaata cggctcccgg agatttggat tcgacattcc atgaggcacg
     1741 atatcgacat actgatcgcc ctgtttcaca gagacaaaca aaaccagtgc cacacaaaag
     1801 ggctgattca caacaaattg cggatccagc aactggaagt tttttgtgga ggatcggcag
     1861 atgtttttcc gcaaattctg ttgcgttatg tccaaatgca caatgcattt tttataacac
     1921 gcgttggggt cacccccatc ggtattccat gcgttaggaa tgggcacatc ctgcgcttga
     1981 ctcaagggta tgtattgagg cccggccaca cctcccgcaa aactggccga gttcaacgac
     2041 gtcccgtcat tcggtatgat tttcataaaa gccttcacct caagcgaata attcggtatt
     2101 gcgctacgct cctgatgatg cttgaacggc caggtcacct cgacctcaat gatacgcgct
     2161 cgagggtccg ttgccgttgt catttgcaga tagtagatcg tctccggcgg atttgtgtcc
     2221 ggaacaagtt cttgcttgat tatcggagtt aatccttgca gcaaccggat ctcgctgacc
     2281 atcggggtca tctcaaccgt cgggatgtca aaccgtttgg gaatgtggtc gttgtcaagg
     2341 taatcggcga tcatcggcgc cataatgact tgttggttgc gcagtcctcc caccagagca
     2401 ttccccaagg cggtttggaa caccggatcc atgctcggaa tcgctgaccc taaatttttt
     2461 atcggctgtt tgttttccgt gatggcgaac gcgttcgcgt tgccgggttc aggtttgacg
     2521 atcccgtcgg tgatcagcac atggtggctt acgaagccga agtccagatc gtcaccggta
     2581 tccagccatt tgtgataggg gcttccgaaa gtcggatcgt tgcgttcgaa catgcaggcg
     2641 tagaaatctt gcagggtttc gtagcgccgg tcgagatttt gtttgttgtt gtcgaacgtg
     2701 ttagccgcac cgtcatttcg gaacaggtgg ccgatattga tgcggttggt gccggactgt
     2761 gcggcactga ccgcattcac gttcagcatg tcggagaggt tgacgcacac aaaagcgaag
     2821 cgcccgactt ccgcgcctcc gaaagcgttc tcgccgctgt tgaagttgat gggcatgctg
     2881 agttggcgcc agatcggacg cgtatccccg gtcaacgctt gataccgcac gtcattcacg
     2941 aagatgccgg gaagaaagct gagggactcc agggaaagta cgcgggtatc ctgaaccgtg
     3001 gcggagaggg tgttggttcc gcccggatag acgcggcccg gccagggcgg gaatttcacg
     3061 tcccgcaggt ggcggtcgac ctcatcgatc gcacgaacga gtcccgcgtt gagcatgtgg
     3121 cgcgcggtga tcgcgtgacg gtagttggag gaggcctggc gttcgatccg catgaagatg
     3181 gcgaacgaga ccgcgctgat catcatgaaa ctcaaaaaac cgagcacgat cagcaacgcg
     3241 gagccgccct gccccacccc gtttcgtccg tatcgaaaaa gtctgttcat ggtattgtcc
     3301 gtaatgtggc tattttatca cacaaagtcc tttaaccttc cctcccctat cgcctatgcc
     3361 ggttggggaa cgtcacggat gcggggaatt catcggtttt cggattctgc cgcgtttcat
     3421 acagcaaccg caaccggtac gggttgcccc acgggtccag aaatatcccg ccggtcaggc
     3481 tggcgttgag gtataccgcc tgttcctcat tttcgcccag cagttcaccc agtgcgcctt
     3541 ggtccgcaat gatctcgtct cccgaaatct ccaccggcca gtcgtcatgc gcggcctcgt
     3601 aggcgagcca ggcgttaatc aactcgcgcg tttcagagtt cgctttggcg attttcgcct
     3661 gttttcgagc ctgccccaca ccggagaagg agaccgccat gagcaccccc agcatgccga
     3721 ccaccaccag catttcgacc aatgtgaacc caagagacgg gaggcgcgag gcgcgaagcg
     3781 tctccgccgg ttttcgcatg ttgttccgat cgtgcataaa accccctctc actcctccac
     3841 ccacgtcttg atatcatccg tcgtgttggg ccggccgtcc ggcccgaagg accaggcgcc
     3901 gatgtccagc gtgttgccgg tgctacgcgc ctgcgcaccg aaggtgacgt ggagcggcag
     3961 gccctgggag tccagcccgt cgccgctctg accgaaatgg ggggtgaact gcagcggctt
     4021 cacacccggc cggtttttgc tgctctggcg ctcggcgaag gtcagcacgt tggccttttt
     4081 cacggcaccc caggaaccgt ccggcttcag cgactgctcc gtgcggttgc ccgcggtgtc
     4141 ataggtgatc tttttgagac tgcggttgaa aagcaggttg tcgttctgat ccagctccac
     4201 gccgccggtc agggtgtaaa agaccagacg gccacccgaa aagttctgct cctccgtcac
     4261 atactgctgc ggcaggagcc gcgcgtccac cgcggccgag acgtcgcgct ggatcgcgcc
     4321 gatgtaggaa cgcacctgca tatacgcctc ggcccggaac gatcccgagc gccaagcaag
     4381 gctggtctgc tggaagagca tcccgagcat catcacgatc accaccagaa tgctggccgc
     4441 gaccagaacc tcgatcatcg agaaaccggc atgggaacgg ttgattgtcg gcagggacgg
     4501 ttccccgaac cgtccgcgga cgccccgtag aggcgtccct gcccgcaacg gccacacatt
     4561 ttccgaaacc gttccagcgc ggcacccgcg ggatttgaga ttggggatct gcaagttgac
     4621 catcgtgccc attacctgtt acctgttcct tataacctct aacttctccc ctatttcgtc
     4681 acgtcacctt ggaaataggc ctcggcataa tagaccgggt tgttggtgta attccggacg
     4741 tcttcgcctt cgatctcggt ggagcggacc atgattccca tgatccggtc cgagccgccc
     4801 gcgccacggc agcagatgat ccggaagcgg tcggaaggcc agtaaggcca gtcatcggcc
     4861 tgccgcatgc gctctctcat aaaggctggt ctgttatccg tctgcgaagc cgtgatgccg
     4921 ctctggtcga gagacgcgat gctggggaca ctcttccact gcgaccacgg aaaattcgtc
     4981 atggacgcca cagccaccgc ctggttcagc aggtaatccg cgaacatcga ctgcgtcagg
     5041 tcctgttggc cctgaacgct ttcgcgcaaa cccaagggat aaagcgcgac catgctcagg
     5101 atgccgaccg ccatgacgaa aatcgccatg ttgacctcca tgagcgagaa tccggcccgt
     5161 acgctgaaag gcctgatcga gccgggatcg cgggcgtccc cgcccgcaac ggcgtaacgc
     5221 tcaacgcggt tccacccgca tctctctgtc attcttccgc gcatcgtcat ctccttgtcc
     5281 gcccaatccg ggtttccttc tcctgctact tgtactggat cgaaccgtcc cgctgcaccc
     5341 ggatctcgct ccgcagtttc ggcggcaaca gttcgcggat cgtcaccgtc atggccttct
     5401 gcgctgtgcc gtccggcagg atggccttct gcgctgtgcc gtccggcagg aacgtgatga
     5461 gctcgacttt gttcagattt tcgggcaagg tatccgaatt accgtccaga ttggtgaact
     5521 tgaacccttt gggcagcgaa ccgggcggcg cggcctcgat accgtagaca tccccgacct
     5581 cccacatcgc atcgttcgcg ttgcggacat tggcgttttt cttgaatccg aaggccggaa
     5641 tcgtatagct gcggtttgcg acatacgacg acctccgccc ggaaaagctg tgggataccg
     5701 ccgtgggcgt gacctgccac cacttgcccg ccgtgaggtt gtacagccgc atgcttccga
     5761 gataagagga accgaccgtg tcatcctccg ttgcggtgcc gaacatctta tcgagcggcg
     5821 tgaactcatc aaccagcgtg gtcccgctga gagcggtcac acggcccgcc tccttgcaca
     5881 ccacgtaaga gggcacgcac tccggatcac ccttattgtt gtatccggcg acttcgttgt
     5941 acaccatcac gctgacgcga accccttcga ggcaggcgcg ctgacgcgcg aggatcagac
     6001 tgttggccag atgcttgacc gcgctgcgcc gcgtcatacc gcggatcgcg ttgaagtaac
     6061 tggtgaccgc cagagtggag agcagggcta caatcgccat gaccgagagc agctcaagca
     6121 acgagaaccc cgcagcggac cggaaaccac gggatttgag atttgagatt tgagatttag
     6181 ccatcgcgtc cctcacttgt ctttgcaggg ggagtggtcg gagtgcggat gcaccgcgac
     6241 ggtgtcagtc tcgacccggt actccacaca gtaatagcga aggatggagg tctcattggg
     6301 atcggcgtag atcaacggca catcccgtct gcccgcgcta agcgcggcgc gaagctgcac
     6361 ccgctgacca tcaagcagcg ccatcaccgc cgacgcatca agatagaccg tcttctgacc
     6421 cgcgccctga tacagatctt tgaagacctt cgcattgtcc gcgccgtgcg cccagtattt
     6481 cttgtcgttg cggctctggc cttccgtacc ctgccgcttc agatcgctca gtttaaacgg
     6541 ccacttgccc tcctgcgcac gataattggt cagggccatc tcaagcgcac ggcaggtggc
     6601 ctcgacccgc ttcttgcgcc cctgtttgac agccttgacc accgcaccgg tcgccagcgt
     6661 ggcgatgacc gccatgatcg aaaccacgac caacagttcg agaatggtga atccggtcga
     6721 ccgcggtttg agatttgaga tttgagattt caccatcgta tcctcttctg gaacttcagc
     6781 gttcattgtt cgtcgttcga tgtccggcgt tcctgttccc tacttgcccg cttccgccct
     6841 ggccttgccg tggttccaac tcaagcggtc atcgtccgga tagcgtctgc cgcctttttc
     6901 ggcgtcgtcc ttgccatcgg ggccgcgcga ccagaccagc accgaagcgc gcaccttcgt
     6961 gccgtcgggg gcctccacat acccgtcgta atcgtcatcc agacggatct ggaggcggtg
     7021 cttgatcacc tcccccgcat tgataccggg gtcgcgccgc aacagggcgc ggccataggg
     7081 atcaagcagt ccgtaccggt cgaggctgtc gttattgatc tccgcgcccc cgcttttgta
     7141 ggtcgtcaca tcgaagagct tcttctcctg cagtacccag caagcctcct catccatttc
     7201 cgatttgttc ggcaacacat ccccccaact ccgttcctgc tgaaggtaga tgttgagcgc
     7261 cgtcgccaac tcggaagcca gactctgggc ctgcgattgg cgcgcggcct tctgcacata
     7321 ggagaacgag ccgaccagcg cggtggcaag gatcccgagg atcccgatca ccagcaacat
     7381 ctcgaccaag gtgaaacccg aacggagtcg gccggaaaca gggggtgccg tgtgaagcgc
     7441 gtgtttgatt cggattccag tcgggagcgc gggcgtccct ccggccccgc ctccataacc
     7501 taagcggtgg ccgtccgcca ccggatgtcg aatgttgttc caataccaca taaacgtctc
     7561 actcatctta catgcggacg aagcccgcga cccaagaagt cctcgcacac ccgttgtctg
     7621 tgagcttgcg gggtggcgca ggccggttgg gctacaccac acccccatgc tcacacactc
     7681 ctcctacttc gaccggtcaa accgggcgat gtcatccttc agccactccg aaacgattct
     7741 ccgatcatct gccgagagcg attccaacga gatccaggga gggaaggtct tcttgtcggg
     7801 accggcagac cagaggcggt agctttgata cggagccgca gaatagtaat acaggtcttc
     7861 cccccaaccg tcccggacgg agacacgcat gagcatatac gtgaccgcgc tacttttggg
     7921 gtcggttgtg ggctccatgc tcagaccggt atcgggctct gtggtgttga tccccaaaag
     7981 gtctccggga ccaccgcaaa cgctcttttc cagattgggt agccagcgcg ccaccgcatg
     8041 gttctcccgg tcccgctgcg cggccagact ctcccggagt ttattccacc acccgccgct
     8101 gggacccgag atcttcgatc ccgcgttgta gtgcgtccat tgcctcgtgt cgaagaagta
     8161 agcgttgggg atgttctcgt tgttctcgct gaggttatcc agctccgaac cgttgagcat
     8221 cagctcgaca cgcggcatca gataggacat cagaccgaac cggaagatcc tgatctgatc
     8281 ccactcataa tcgttccggt tgcgaacatc cggaccaaag gcatcgttca cgctcacggc
     8341 gccctcgctc tgatagcgtt cgttgatgta aaaatcaagg gaacgggcat tcggataaca
     8401 gaaggcgacc ggctggcagc gcgcggcgcg gttggcgttc gtcgggttga gctcagcact
     8461 tcccgtgtag gtgccatcct tgtccgcctc catatagggg tcgggtgaac cgtgggacgg
     8521 cacgggcgga taggtgccga actccgcata gaatccggaa atggcgttct gcatccgctc
     8581 cagacgggcg atggtcttcg cgcgctgcga actggtgccc gcagtgctga gcagcttgaa
     8641 caccccgccg atcagaatgc cgatcaccgc gatcaccacc atcatctcga tgagcgtgaa
     8701 tccgcgggac acgccgatct ttcgcacact gtttctcgca ttccgcataa aaactctttc
     8761 tcccgcggac ggttcaggga accatccctg tcggccggtt agccattaac ccgtaaccta
     8821 ttataacctg ccccttacgc ctgtgcgccg aggctgccga tgatctggat catgggcatg
     8881 aacatggcga tcacaatcgt accgacgact accgccaaaa taatgatcat gatcggttca
     8941 atcaccgagg tcagccccgc gaccgcattg tccacctcat cgtcataggt ttccgcgatc
     9001 cgcgtcagca tgtccggcag ggcaccggtc tcctcgccga cctccaccat cgagatcacc
     9061 atggggggga agaccttgca ggccgcgagc gggttggtca tgctctcgcc ctccttgacc
     9121 gcgtcatgca cgttctgcat ggcgcgggag atcacctcgt tgcctgtggt atcgcgcacg
     9181 atgttgagcg cctgcagcac cggcacaccg gaggccagca gggtgccgag cgtgcgggtc
     9241 atgcgggcga tggccgtgcg gcgcaccagc gagccgaaca tcggggcatt gatcttggac
     9301 acatcgagca gataccgccc gaagcgcgtc ttggcgaaaa tcttaaacaa cacgaacagg
     9361 gccgcgaaag ccgcgaccac cgccaagccg ttgttcatca ccagattact ggcgtccatc
     9421 acaaactgcg tgatggccgg caacggcttc ccttcgagca gatcattaaa gatatcctta
     9481 aacttcggga tcaccgcgac cagcaggaac ccggtgatgc cgacagcagc cacaaggacc
     9541 acgacggggt aaaccatcgc gcttttgact ttgttcttga ttttggccgc cttttcagcg
     9601 aattcggcca agcggttgag caccacttcc agaacgccgc cggcctcgcc ggccttgacc
     9661 atgttgacat acagattgtc gaagatcttg ggatagttgg tgagcgactc cgagaaggta
     9721 ctgccgctct cgaccgattc acacatcccg gccagggcat ccttgagcgc cgcgttgggc
     9781 gtctgacgct ggagcacatg caacccgcgc agcagcggca accccgcgtc caccagcgtg
     9841 gcgagctgac gcgtgaacgt cgtcaggtcc ttctgcttca ccttgccgcg caggaacttg
     9901 gggagtttga tctcgattcg ggtctgagcc aaggatttct tcgccgcagg acttttttta
     9961 gccgctgccg cgggccgggc cgttgcggca tccgatcctc ccgcaacacc gatggcagtg
    10021 ggaaacaacc cctggctacg caccttagcc acggcctgag cctgattcgg ggcttcaatc
    10081 gtgccgcggc tctccttgcc tgcggcatcc acagcgatat acgcaaattt gggcatacgg
    10141 caactccgga tagtttggac ccctcgcaca catgcattcc gaatccgtag agaaagatat
    10201 catagtctgc acttgggcgg caaacgtaaa agagaagcgc attgtaatcg gttcgtgaca
    10261 ggggtgcgat cgaaggtcat gcgtttcagg gttccattgc ggcgccatcg ccatcgggat
    10321 cgggatcgga atcgggatcg ccaccatcat tacacttgtc gctacgggcc actgcgcggg
    10381 cgcaacagtc gatttcgaac ccgatagcga taccgatacc gatagcgata ccgataccga
    10441 tttggagaac gtgaatgttg gctatacccc cgtacccg
//
LOCUS       DAOSLE010000090        10472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6215_length_10472_cov_8.237304, whole genome shotgun sequence.
ACCESSION   DAOSLE010000090 DAOSLE010000000
VERSION     DAOSLE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6215_length_10472_cov_8.237304"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PK576_07195"
     CDS             complement(<1..601)
                     /locus_tag="PK576_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOE61415.1"
                     /translation="MRREIHIAGIGGVGMSALAQALLDQGERVSGSDRLLDAGDETGV
                     LTCLAAQGVRLCRQDGTGITPQTERLVISSAIESDNPEVLRAQALEVPVVHRAAELAR
                     LTEGRRLIAVTGTSGKSTVTAMLGCLLEGAGFDPLVVNGAAVAGWSAEGTRIGSVRKG
                     AGEWAVIEADESDRSLMVFSPEHAIITNASADHFGLDETL"
     gene            complement(616..2247)
                     /locus_tag="PK576_07200"
     CDS             complement(616..2247)
                     /locus_tag="PK576_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOE61416.1"
                     /translation="MRVMAAVGLWLDRQPLLAATVCLLAGIMCGYRVVGVWWAVIVAA
                     ALLCAYMAVFTRTPLRSIFVALCFLLVGWALAARDRAGRDDETRRFRALSQPQTYVCR
                     VGPEVTVQPLRGRAAKHTFRAERLRTEDGAFACRHLPVEVTWFGAAEGAAGATPQPGE
                     IWRIRGSGRVRKKRNGLLQLTLNTGEGRASRLAAISPSAWRARVANARRTASRRVVIG
                     IETWGDIPALNQAMLLGNRHVMPPALRRVFVDSGTIHVYSISGLHIVLVAAVLSLAVA
                     ACGIPRTYWVLASGPLLIFYTALTGARPPAVRACLMALLYLAAPLIGRRPNALMALAG
                     TALIVHAMQPWLIFDIGCVLSFTVMGGLVAFCRPFCQAGQRLFRVARLTERANLARAA
                     GAKMRARRLNMLARAITWVSDSFAVSLAAWAASLPLTAHYFGRFTPGGLFANLAVGPC
                     SFFIVVASCLGVMTSTVSEWVAGCFNNAAGFFTCVMVKAAQITVAFPGANLRIKPWPL
                     WRVWLWFGVLLVLAAWLNTRRRRPDGLAWLTDTQW"
     gene            2661..4085
                     /locus_tag="PK576_07205"
     CDS             2661..4085
                     /locus_tag="PK576_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOE61417.1"
                     /translation="MNEKSRVPAYLECAQPNPAANRAPWYKNIAPSYAGVFLWFVFWS
                     GTASLLGGNAGGIFAAGWPVALLGIIAGGAICYLLFYHVFGCLGQKTGLPLYIVGSST
                     FGAKGGFFLPGFLMGLLQFGWVAVNIAGAAYALNLFLKLPPGSPWLYAIMIVWGVASV
                     VMALKGIKYVAMISTWLPIVPALVLLAALAKTIGGLGSFDAAAFVAAAKAGNPDALPA
                     LGGTAIIFALITHIVGFTATAGAAGVDFGTNARNKKDVVMGGLVGILGAIVLTAGVAA
                     LIVAGFYGTAAGKELMAQGKVVLDPVALLANLFGDKAGGVLAFLLAICAFPSCCFSAL
                     IAANSIKTTLPKINPWISCGIGCVIAEILAMTGVALKLPVIFGFFGASFGPICGAMVA
                     EYFINKGRWSGPRAGFNPAGWIAWLVGFVIGAQPQLVAWGLPLGKPLPLSLILAFFAG
                     AVIYAQLAKIQTPVLPYPQASPER"
     gene            complement(4129..7371)
                     /locus_tag="PK576_07210"
     CDS             complement(4129..7371)
                     /locus_tag="PK576_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61418.1"
                     /translation="MIKGFQEVSAQRRKIDGWLSFGFGLLAAGVYGLTVSRGVYPGES
                     ARLMAICSGIETAELPLHPVWGTLAGAVSRLSFFTLPVRMNLLSVVCGVIAVMLLYRV
                     MAFLIRNTLYEEYSIEYAPRVATLAASVTVLAFLFSVPMWKASTRLHYQSFDILLALV
                     SAYLLVCYANSGWRLFLVVFAVLQGIGVVESVLFIPLAPVFLVFLIYVLWKREALTLT
                     RVAWIGVLAVAGLSLYYVAARRFLDAQGSALGFSSVMDVLVQVWKNQIRQLRSGLPRV
                     GWLVLILMSVVPWVASGLTAFRALNNERSWSQYILHITLSVLVVLGLANAEISPWEII
                     KPTGRLPVCSYLMLSMTAGYLVAYWYLLLVVKRPRQGHEVKAITQQTGDWLGLIFTYP
                     LALFVVLAALINAFECGGQSGAFADRCAREVLDRLDGRTWFVTDGTLDPHLQILARER
                     GQELNLLCLQKDMSPHYLQFLAKMVEEKQMFEPTERQRMKNTLELGVLPFIQDWFAMD
                     REIEKKVAVFGVPDFWYTAGITPVPEFFFFSGCRDIRTLQDRPLLEEYKAFWKEMDVP
                     LARDKKGSDDPTVRLRAHLRRHMGFVANNLGVLLEDLGKDAEAFAVYTIVHDQIDPDN
                     VSAMFNRFEMARRGVSAANPFKESIERELKDFIGNLKQKYPLWSLSRYYGYVRSPEIF
                     ARLGWGWALSGQTGAALAGVTRAINLLPTDQRVNAMQAMAAVYALSEDRNKTEAVYQD
                     IIRSDPKNRAAMLGLARLAVRDGALDKAKQWLEKAAQSDDRKGSLGVEWAVIHLMNND
                     IGRARLALQELTDLQPKNLQAWAMLAMVQLQQKEDADVEKVILPKMESIAGTPDDYFI
                     QITRAQVAMRKGKNFQRVAREGFIRASMLRPDIPGVKDMILQLDIAMNDQEAAELHAR
                     QVLRVNRKHALANYVMGSLRLQEGAYGEAEDFLRRSVEAKPSAAALNDLAEVLRRIRR
                     FDDAEAQIRAAIKMNAELYVAWETLGAILLDAGKDLDEAERAVNQAISLYADDLRVKI
                     TLARILLKKGEIERARETIRLLRNRQGELSRFDQEVLARLVEEASGRKAR"
     gene            complement(7491..10229)
                     /locus_tag="PK576_07215"
     CDS             complement(7491..10229)
                     /locus_tag="PK576_07215"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOE61419.1"
                     /translation="MLKRVCGMMAVLTAGTVCAGPAGFWRFEEGKSGETAGSVASAAN
                     AQVLAGKAGVFEKGTPPVFDAEVPHKVLWDGATATLAAPTNGVSLRFRAGEVTGNGSP
                     VGGEVVVSGKEAAVKSASFTMEAFVKVARLTTRHALVVSKRRNGQTGASWALSVTPGG
                     TLSARFDTQPGAGSGNGFNQCFGASSSLNDGQWHHVALTFEEASRKVGLYVDYQLCGK
                     GITKGTLVYDDSDLVIGRGLDGWLDEVRLSPEALHPEQFLRPERFFSEMKLRVAYVPL
                     LDQTHSRVQSALKPDLKKLGTLIPKSVPEIETTMWSLGCETLDRNLADWDAYREYLVP
                     LGIKRIRLQGGWNRTEKVKGVYNFAWLDHIVDDAISLGLEVCLETSYNNRLYEPNGAT
                     GPGGHLPSGEETLAAWDKWVEAMARRYSAKGVNEWMMYNEPNLKRDFNTPERIVENNI
                     RTAEIIKRINPKAKVGAFVLAGLGVESMETMLTMIKERGKLDLFHWAVYHGYSGNPDR
                     LCERMGQFNAMLKCVAPSIRPWQGEAGCASEEVQYALSGIDWTEYSHAKWNARRMLCD
                     VGCGIESSVFTISDLSYHKDFISRYGLLKTNPDNSLIKVKSAYYTVQNVVTLFNDALT
                     LTPEREVTVTGTERRISKFAFRDKKSGLDVIALWDGTEIPGNGSEILPVQVAVKGGHF
                     KDPVWVDLITGVVYAIVPEQMKAEGGTVTFTNIPLYDGPAAILDRSLVNYEPVKVVRK
                     KKAKAVKSAKPAQGQKPAKAGASPVVAHLLPGTQQPAPAVLILGMKDADAGELITWLN
                     GQDVHAFVLAKDEKPVEGSAEKVKGALAAIRGRAAEWQVKTDAVGVMGQGATGAAAAT
                     VAGGHDADFVILIDGADAQASKMAVGAKRVPLFVGKKDAWQTPLAAWLEKFKGKVF"
BASE COUNT         2017 a         3654 c         2916 g         1885 t
ORIGIN      
        1 ccagcgtttc atccagcccg aaatgatccg ccgaagcgtt ggtgatgatc gcgtgctcag
       61 ggctgaagac catcagcgag cggtccgatt cgtccgcttc gatcaccgcc cactcgcccg
      121 cgccttttct gaccgatccg atacgcgtac cctccgcgct ccagcctgcc accgccgcgc
      181 cgttgaccac cagcggatcg aaccccgcgc cctccagcag acagcccagc atcgccgtca
      241 ccgtgctttt gccgctggtg ccggtcacgg cgatcaggcg tcgcccctca gtcagccgcg
      301 ccagttcggc cgcgcggtgt acgaccggga cctccaatgc ctgcgcgcgc agaacctccg
      361 ggttgtccga ctcgatcgcg ctgctgatca ccaaccgctc ggtttgcggc gtgatgcctg
      421 ttccgtcctg ccggcagagc cgcacaccct gcgccgcgag gcaagtcagc accccggtct
      481 cgtcccccgc atccagcagg cgatccgaac cgctgaccct ttcgccttga tccagcaacg
      541 cctgcgccaa cgcgctcatc ccgacgccgc cgatacccgc aatgtgaatc tctcgccgca
      601 tgtcacacct ctccatcacc actgtgtgtc cgtcagccat gccaacccat ccggacgacg
      661 cctgcgcgtg ttcagccagg ccgccagcac cagcaacacg ccgaaccaga gccaaacccg
      721 ccaaagcggc caaggcttga tgcgcaggtt cgccccaggg aacgccaccg taatctgggc
      781 ggccttaacc atcacacatg tgaaaaaacc ggcggcgtta ttgaagcaac cggccaccca
      841 ctcgctcacc gtactcgtca tcactccgag acagctcgcg accacgatga aaaacgaaca
      901 cggccccacc gccagattcg cgaagagccc gcccggcgtg aaacgtccga aatagtgggc
      961 ggtcaagggg agcgacgccg cccaggccgc cagcgacacg gcaaacgaat cggacaccca
     1021 tgtgatcgca cgcgccagca tgttcagacg gcgcgcgcgc atctttgcgc cagcggctcg
     1081 cgccagattc gcgcgctcgg tcagacgcgc aacgcggaac aaccgttgcc ccgcctgaca
     1141 gaagggtcgg caaaaggcca ccagcccgcc catcacggta aacgagagca cacagccgat
     1201 atcaaagatc agccaaggct gcatggcatg cactatcaac gcggtgcctg ccagggccat
     1261 cagagcgttc ggccgacgcc cgatcagcgg cgcggccaga tagagcaacg ccatgaggca
     1321 ggcgcgcacc gcgggcggcc gtgcaccggt gagcgccgtg tagaagatta aaagcggacc
     1381 gctcgccaaa acccaatagg tgcgcggaat gccgcaagcg gcgaccgcga gactcaacac
     1441 ggccgccacc aaaacgatat gcagaccgga gatcgagtag acgtgaatcg tgccggaatc
     1501 cacaaagacg cgacgtagcg caggcggcat cacatggcgg ttgcccagca gcatcgcctg
     1561 attgagcgcc gggatatcgc cccacgtctc gatgccgatc accacgcgcc gggatgcggt
     1621 gcgccgggcg ttcgcgacac gcgcacgcca agccgacgga ctgatcgccg ccagccggga
     1681 ggcgcgcccc tcgccagtgt tgagggtcaa ttgcagcaaa ccgttgcgct tcttgcgcac
     1741 gcgccccgaa ccgcgtatgc gccagatctc gccgggctgc ggcgtcgcgc cggctgcccc
     1801 ttctgccgcg ccgaaccaag tgacctccac cggcaggtga cggcaggcaa aggcaccgtc
     1861 ctcggttcgc aacctttcgg ctcggaaggt gtgcttggcc gcgcgtccgc gcagcggctg
     1921 gacagttacc tccggaccca cgcgacagac gtaagtctgc ggctggctga gcgcacggaa
     1981 gcggcgcgtc tcgtcatcgc gaccggcgcg gtcacgcgcc gccagcgccc aaccgaccag
     2041 cagaaagcag agcgcgacaa agatgctccg tagcggggta cgcgtaaaga ccgccatata
     2101 cgcgcacagc agcgcggcag caacgatgac ggcccaccac acgccaacca cccggtaacc
     2161 gcacatgatc ccggccagca gacacaccgt agcagccaat agcggttggc ggtcgagcca
     2221 gagacccacc gccgccatca cccgcatgta agggttcctg aagccgcgca cgtttctccc
     2281 gttttctcag ccgactgtca tcaatgatcg tcatggtact agagtcaggc gcgccccgca
     2341 acggtaatcg gttagtgaca ggcttatcgc gaccacccag tagtcgcaat gatgaatgta
     2401 aaaacgggcg gggcacggct gcatggcgca aggttttaac ctcgtctttg tgaccactgt
     2461 gtggccacaa aaagtggcta tggtttcctt gcggcaccat cagtatcgcc atcggtatcg
     2521 ctatcgctat cgggatcgga atcgcacttg acccgctttg acacattgcc tacgctatgg
     2581 cgtcatcgtt cgccggaaac gggggcatgc ggcgcaggat cgccgcagaa acccgacacc
     2641 accataaaaa ggagccttgt atgaacgaga agagccgtgt gcccgcctat ctggagtgcg
     2701 cgcaacccaa tccggccgcc aaccgtgcgc cgtggtacaa gaacatcgcc ccgtcttacg
     2761 cgggtgtctt cctgtggttc gtcttctggt ccggaaccgc ctcgcttctg ggcgggaatg
     2821 cgggcggtat tttcgcggcg ggctggccgg tggccctgct cggcatcata gcgggcggcg
     2881 cgatctgcta cctgctcttt taccacgtct tcggctgtct gggccagaag accggcctgc
     2941 cgctctacat cgtcggctcc tcgacctttg gcgcgaaggg cggttttttc ctgcccggct
     3001 tcttgatggg actgttgcag ttcggttggg tggcggtcaa tatcgcggga gcggcctacg
     3061 cgctgaacct tttcctgaaa ctgccgcccg gatcgccgtg gctctacgcg atcatgatcg
     3121 tatggggcgt ggccagcgtc gtgatggcgc tcaaaggcat caagtatgtg gcgatgatct
     3181 ccacctggct gccgatcgtg ccggccttgg tcctgctcgc ggcactggcc aaaaccatcg
     3241 gcggactcgg ttcgtttgat gcggctgcat tcgtggcagc ggccaaggca gggaatccgg
     3301 atgcgctgcc ggcgttgggc ggcacggcga tcatctttgc gttgatcacg cacatcgtcg
     3361 gctttaccgc caccgccggc gcggcgggcg tggacttcgg aaccaacgcg cgcaacaaaa
     3421 aagacgtggt gatgggcggt ctggtcggca tcctcggagc gatcgtcctg accgctggcg
     3481 tcgcggctct gatcgtcgcc ggtttctacg gcacggcggc tggcaaagag ctgatggcgc
     3541 agggcaaggt ggtgctggat ccggtggcgc ttctggccaa cctgttcggc gataaggcgg
     3601 gcggtgtgtt ggccttcctg ctggccatct gcgccttccc gagctgctgt ttctccgcgc
     3661 tgatcgcggc caactccatc aagaccacgc tgcccaagat caacccctgg atctcctgcg
     3721 gcatcggatg cgtgatcgcc gagattctgg cgatgaccgg cgtggcgctc aagctgccgg
     3781 tgatcttcgg tttcttcggc gcttccttcg gaccgatctg cggcgcgatg gtcgccgagt
     3841 acttcatcaa caagggccgc tggagcgggc cgcgcgcggg cttcaacccg gcaggctgga
     3901 tcgcttggct ggtcggcttc gtaatcggcg cgcaaccgca gcttgtcgcc tggggtctgc
     3961 ccctcggcaa accgttgccg ctgagcctga ttctggcctt cttcgctgga gcggtgatct
     4021 acgcgcagct ggcgaaaatc cagacgccgg ttctgcctta tccgcaggcc tcacctgaac
     4081 ggtgagcgct tttaccctca ccgttcccga aaaagcggac gcatcagcct accgggcttt
     4141 gcgtccggag gcctcttcca cgagacgggc caacacctcc tgatcgaacc gggagagttc
     4201 gccctgccgg ttccggagta gccggatcgt ttcgcgcgcc cgttcgatct cccccttctt
     4261 gagcaggatg cgggccagcg tgattttgac gcggaggtca tccgcgtaca ggctgatcgc
     4321 ctggttgacc gcgcgttccg cctcatcgag atccttgcct gcatccagca gaatcgctcc
     4381 cagcgtctcc cacgccacgt acagctcggc gttcatcttg atggcggccc ggatctgcgc
     4441 ctcggcgtcg tcaaaccgac ggatccggcg cagcacctct gccagatcgt tcagcgccgc
     4501 ggcactcggc ttggcttcca cactgcggcg cagaaaatcc tccgcctcgc cgtacgctcc
     4561 ctcttgcaga cgcagcgatc ccatcacata attcgcgagc gcgtgtttgc ggttgacgcg
     4621 caacacctga cgggcatgca gttccgccgc ctcctgatcg ttcatcgcga tatccagttg
     4681 cagaatcata tccttgacgc cggggatatc cggccgcagc atggaggccc ggatgaatcc
     4741 ttcgcgggcc acgcgctgga aattcttgcc cttgcgcatc gcgacctgcg cgcgcgtgat
     4801 ctgaatgaaa taatcgtccg gggttccggc gatcgactcc atcttcggga gaatcacctt
     4861 ctccacatcc gcatcctctt tctgctgcaa ctgaaccatg gccaacatcg cccacgcctg
     4921 caggttcttg ggctggaggt ccgtgagttc ctgcaacgcg agccgcgccc ggccgatatc
     4981 gttgttcatc agatgaatga ccgcccactc gacacccagc gagcctttcc ggtcatccga
     5041 ttgagccgcc ttttccaacc actgtttagc tttatccaac gcgccatccc gcaccgcaag
     5101 acgcgcaagc ccgagcatgg cggcgcggtt tttcgggtcg ctgcggatga tgtcctgata
     5161 caccgcctcg gttttgttgc gatcctccga cagcgcgtac accgccgcca tggcctgcat
     5221 ggcgttgacg cgctgatccg tgggcaacag attgatggcg cgcgtcaccc ccgccaatgc
     5281 ggctccggtc tggccggaca gcgcccaacc ccatccgagg cgcgcgaaga tctccggcga
     5341 ccgcacatac ccgtaataac gcgacaggga ccacagcgga tatttctgtt tgagattgcc
     5401 gatgaaatcc ttgagttcgc gctcgatgga ctctttgaac ggattggccg cgctgacccc
     5461 ccggcgcgcc atctcaaagc ggttgaacat ggccgacacg ttgtcgggat cgatctggtc
     5521 atggacgatc gtgtaaacgg caaaggcctc cgcatccttc ccgagatcct caagcagcac
     5581 accgaggttg ttcgccacaa aacccatatg ccgccgcagg tgagcgcgca agcgcaccgt
     5641 cgggtcgtcg ctcccctttt tgtcgcgggc cagcgggacg tccatctcct tccagaacgc
     5701 cttgtactcc tccagcaacg gccggtcttg gagagtccgg atgtcacggc atccactgaa
     5761 aaagaaaaac tccggcaccg gggtgatccc ggcggtgtac cagaaatccg gcacgccgaa
     5821 aaccgccacc ttcttctcaa tctcgcggtc catcgcgaac cagtcctgaa tgaacggcag
     5881 cacgccaagc tccaacgtgt tcttcatccg ctgccgttcg gtcggctcga acatctgttt
     5941 ctcttcgacc atcttggcga ggaactggag ataatggggg ctcatgtctt tctgcagaca
     6001 aagcagattc agctcctgcc cgcgttcgcg cgccagaatc tgcagatgcg gatccagcgt
     6061 gccatctgtc acgaaccagg tgcgcccgtc gagccggtca aggacctccc gcgcacagcg
     6121 gtcagcgaac gcgccgctct ggccgccgca ctcgaaggcg ttgatcagcg ccgccagcac
     6181 cacaaacagc gcgagcgggt aggtgaagat cagcccgagc caatccccgg tctgctgggt
     6241 gatcgccttc acctcatgcc cctgacgcgg acgtttcaca accagaagca gataccagta
     6301 ggccaccaga tacccggccg tcatggacag catcaggtaa gagcaaaccg ggaggcgtcc
     6361 ggtcggcttg atgatctccc acggcgaaat ctcggcattg gccagaccca gcaccaccag
     6421 cacactcaac gtgatgtgca ggatatactg gctccaggaa cgctcattgt tgagcgcgcg
     6481 gaaggccgtc agcccgctgg ccacccacgg caccacgctc atgaggatca gcaccagcca
     6541 tcccacgcgc ggcaacccgg aacgaagctg gcggatctga tttttccaga cctgcaccag
     6601 cacatccatg acactggaga accccagcgc actgccttgc gcatccaaaa accgccgggc
     6661 cgcaacatag tagagggaga gacccgccac cgccagcacg ccgatccacg cgacccgcgt
     6721 gagcgtcaac gcctcacgtt tccacagcac ataaatcagg aataccagaa acaccggagc
     6781 gagcggaatg aagagcaccg actccacgac cccgatcccc tgcagcaccg cgaacaccac
     6841 caaaaacaac cgccatcccg agttcgcata gcacacgagc agatacgcgg aaacaagcgc
     6901 gagcaggatg tcaaaagact ggtaatgcag gcgcgtcgag gctttccaca tcggcaccga
     6961 gaagagaaag gccagaaccg tgaccgatgc ggccaacgtg gcgacccgcg gggcgtattc
     7021 gatcgagtac tcctcgtaca gggtgtttcg gatcagaaac gccatgaccc ggtagagcag
     7081 catcacggcg atgaccccgc acacaacgct gagcagattc atccgcaccg gcagcgtgaa
     7141 aaaagacaat cggctgactg ccccggccag cgttccccaa accggatgca agggcagctc
     7201 cgccgtctcg atcccgctgc atatcgccat caggcgcgcg gactctccgg ggtacactcc
     7261 gcgcgacacg gtcaacccgt aaacgcccgc cgccagcaac ccgaagccga acgacaacca
     7321 accgtcgatt ttacgccgtt gcgcagacac ctcttggaaa cctttaatca tggctcatca
     7381 accttgcttt gaagaaaaac gaccccttgg cttaatgcaa accgactgat ctgtatcatg
     7441 acagaaaagt ataacggttt gaccccctct ccgcaagtcc ggcaatcggc tcagaaaact
     7501 ttgcctttaa acttctccag ccacgctgcg agcggtgtct gccacgcatc tttcttgccg
     7561 acaaaaagcg gcacgcgttt cgccccgaca gccattttcg aagcctgcgc atccgccccg
     7621 tcgatgagaa tcacaaaatc cgcatcatgg ccccccgcta cggtggccgc agccgcaccg
     7681 gttgctccct gccccatcac acccacggcg tcggtcttca cctgccactc cgccgcgcgg
     7741 ccgcggatcg ccgccagcgc gcccttcacc ttctcggcac tcccctccac cggtttttcg
     7801 tccttcgcca gcacaaaggc gtggacgtcc tgcccgttca gccacgtgat cagctcaccc
     7861 gcatccgcgt ccttcatgcc gaggatcagt accgcgggcg cgggctgctg cgttcccgga
     7921 agcagatgcg ccaccacagg cgaggcgccg gccttggccg gcttctgccc ctgcgccggc
     7981 ttggcgctct ttacggcttt cgcttttttc ttccgcacga ccttcaccgg ctcatagttc
     8041 accagactcc ggtccagaat cgccgctggc ccgtcgtaca ggggaatgtt cgtgaacgtc
     8101 accgtgccac cctccgcctt catctgttcc ggcacgatcg catacaccac gcctgtgatc
     8161 aggtccaccc acaccggatc cttgaagtga ccgcccttga ccgcgacttg gacgggaagg
     8221 atctctgagc cgttgccggg aatctcggtg ccgtcccaaa gcgcgatcac gtccagaccg
     8281 ctcttcttat cgcggaacgc gaatttcgag atccgccgct ccgtgcccgt caccgtcacc
     8341 tcacgttccg gcgtgagcgt cagcgcatcg ttgaagagcg tgaccacgtt ctgcaccgtg
     8401 tagtaggccg acttcacctt gatgagagag ttgtcagggt ttgtcttgag cagtccgtac
     8461 cgtgagatga aatccttgtg gtaggagagg tccgagatgg tgaacacgga cgactcgatg
     8521 ccgcacccga catcgcacag catccgccgc gcgttccact tggcgtggga atactcggtc
     8581 cagtcaatgc cggaaagcgc gtactgcacc tcctccgagg cgcagcccgc ctcgccttgc
     8641 cacggcctga tcgacggcgc gacgcacttc agcatcgcgt tgaactgccc catccgttcg
     8701 cacaagcggt ccgggttgcc gctgtacccg tgatagacgg cccaatggaa cagatccaac
     8761 ttgccgcgct ccttgatcat cgtcagcatc gtttccatcg actccacacc cagcccggcc
     8821 agcacgaagg cgcccacctt ggccttcgga ttgatccgct tgatgatctc agccgtacgg
     8881 atgttgttct cgacgatccg ctccggcgtg ttgaaatccc gtttcagatt cggctcgtta
     8941 tacatcatcc actcgttcac gcccttggcc gaataacggc gcgccatggc ctcgacccat
     9001 ttgtcccacg ccgccagcgt ctcctcgccg gaaggcagat gcccgcccgg ccccgtcgcg
     9061 ccgttgggct cgtagagccg gttgttgtag gaggtctcca ggcatacctc cagccccagg
     9121 ctgatcgcat catccacaat gtggtcgagc catgcgaaat tatagacgcc cttcactttt
     9181 tcggtccggt tccatccgcc ctgcagccgg atgcgcttga tgcccaacgg caccagatac
     9241 tcgcggtagg cgtcccagtc cgccaaattc cggtccagcg tctcgcagcc cagcgaccac
     9301 atcgtcgtct cgatctccgg aacgctcttg gggatcagcg tgccgagttt cttcaagtcg
     9361 ggctttagcg ccgactgtac gcgcgagtgc gtctgatcca gcagcgggac gtaggcgacc
     9421 cgtagcttca tctccgagaa aaagcgctcg ggccgcagga actgttcagg gtgcagcgct
     9481 tcgggcgaca acctcacttc gtccagccac ccgtccagcc cgcggccgat caccagatcg
     9541 ctgtcgtcgt acaccagcgt tcccttcgtg atgcctttgc cgcacagctg atagtccaca
     9601 tataaaccga ccttccgcga tgcctcctcg aaggtcaacg ccacatggtg ccactgcccg
     9661 tcgttcaacg aactcgaggc gccgaagcac tggttgaagc cgtttccgct cccggcaccc
     9721 ggctgcgtgt cgaagcgcgc actcaacgtg ccgccgggcg tgaccgagag cgcccaactc
     9781 gcgcccgtct gcccgttacg ccgcttgctc acgaccagcg cgtggcgcgt ggtcagccgg
     9841 gccaccttta cgaaagcctc catcgtgaaa ctggcggact tcaccgccgc ctctttgccg
     9901 gataccacga cttccccgcc aaccggcgaa ccgtttcccg tcacctcccc cgcgcggaac
     9961 cgcagggaca ctccgttggt cggcgcggcc aatgtagccg ttgccccgtc ccaaagcacc
    10021 ttatgcggca cttccgcatc aaaaaccggc ggcgtcccct tttcgaaaac ccccgccttc
    10081 cccgctaaca cctgcgcgtt cgccgccgac gccaccgaac ccgctgtctc accggacttc
    10141 ccctcctcga accgccagaa gcccgcaggc ccggcacaga ccgtgcccgc cgtcaacacg
    10201 gccatcattc cgcataccct tttcaacata tccgccccct ttttacctcg ccgacaatga
    10261 atccggattc ctgttgccaa cactgtacac gaatcgcggt catttgaata cacacggttg
    10321 cgcaaaaaag aacgtccttt ttgcgcacag actggtgtgg cccaagaagg aggaaccccg
    10381 tctattttga aatcggctcg accagcagca gcgaaaccgc atacggctcc aacaggaacc
    10441 gccgcgtcac ctcagccccc cggccgtccg ct
//
LOCUS       DAOSLE010000091        10412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6265_length_10412_cov_7.743652, whole genome shotgun sequence.
ACCESSION   DAOSLE010000091 DAOSLE010000000
VERSION     DAOSLE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6265_length_10412_cov_7.743652"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..1587
                     /locus_tag="PK576_07220"
     CDS             307..1587
                     /locus_tag="PK576_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61420.1"
                     /translation="MKGSVAVSRRAFLAGAGALASVPSITLGQSPRVFKYAVVGCGGR
                     GRGAVRDIQAAAERLGHKAKLVGAADFFKEKAEAVCKSNDCDTKFAFDGAAGYRKVME
                     TDAEIVLLAAPPIFRPLHLEACVRAGKHIFAEKPIATDPAGLRHFLKGVEEAKAKKLS
                     ILSGTLHRHSNRFLRQIKPIREGAIGRILAGQVYRCHGGIWVRPRREGESNAGYLCNN
                     WYHFWEMSGDQITEQAIHEIDLANWFIGRFPKTAMAIGARHRRPAGIGNIYDCMAIDY
                     DYGDAVHVQAIGRHIKGCSDRCGTLLVGSEGEVACGGKITRFDGKPVAYDEERIRGRH
                     ENGMIMEHADFLQGILSGELLHEGEQVAMSTATTIMGTLSAYSGRTVRMSDLLENKES
                     EFYSMNSAFTAADFERGTDIPMPAEGVAAVPGKA"
     gene            complement(1763..2809)
                     /locus_tag="PK576_07225"
     CDS             complement(1763..2809)
                     /locus_tag="PK576_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE61421.1"
                     /translation="MKRTFTHLALLATLLSSAFVVADTPVDMTRRAEELKSLRWGLFI
                     CWSFSTFSGKEWTPGIKDLAFFRATEVDTDQWARTAKEAEMGYILFLTKHHDGFCLWD
                     TRTTDRKVTKAPLGRDVLAELKKSCDKYGIKLAFYFSEGEWRHPGGLDDPATPGPKKN
                     NQGGRDPEMKKAQLKELLTQYGPIEYIWFDHAVGDGGLSHAETLAFCKSLQPGCFIGF
                     NHGDQTGADIRLGEMGKPGSLDDHSAAAPYMRNAPSKNYRLAEFTYPILPPHKGGARW
                     FYSLPEHDGLCHTPEKLYRDYLGAARYGNLFSINVGPDYSGKLREIDVRTLRQIGAWI
                     RNPPPPAQVVTRID"
     gene            3016..4197
                     /locus_tag="PK576_07230"
     CDS             3016..4197
                     /locus_tag="PK576_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOE61422.1"
                     /translation="MFNAYSPELRQDLRFRWNRLQENLRETGADALLAATNVNLLYLT
                     GRIFMGYVHLPAEGEPLFFVRRPCGLSGANVFELRKPEQIPDLLREAGIALPRHLLLE
                     GDDLTFNEHARLTACFPDARIGNGSTDLRQCRAVKTPYEIRIMRLTGSRHAAAVDRFA
                     SVYEPGMTDQAWSIEMFRLMLQAGGLGLFRIAGNSMEGFMGTVLAGDNGGAASPYDFA
                     LGGAGLDPSLPVGQSGVTLTEGMSVMVDIAGNFYGYLTDCTRTFAIGRLAPRAVEAHC
                     RSIEIQQAVAAAGVPGARCEDLYALALKMAEDAGFADCFMGLAQKAKFIGHGTGLVIN
                     EQPVLGARSKETLQEGMCIALEPKFVIPGTGAVGVEDTFLVTPAGMENLTPCDPSMRV
                     L"
     gene            4272..5702
                     /locus_tag="PK576_07235"
     CDS             4272..5702
                     /locus_tag="PK576_07235"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61423.1"
                     /translation="MGRNGFSRRGFLAAGVAWGFPAIIPSRVLGSQAPSKKIQVGVIG
                     CGRIAGGMDIPGLWHNQDLAVPVALADCDIKRLNTTRAKTARLYDGDLPDLKLYQDYR
                     ALLADKTVDAVMICTPDFWHAQQCVEAAFAGKDVYVQKPLAMSVFESRTIVDVMRRTG
                     RVFHIGTQQRSEGKSTFGPQFRKAAEYVRNGRLGRVRRVEIGLPQEPEEPADWPLVQP
                     VPDTLDYDQWLGYTPAELYSELRTHPQGKGAEADFGRPGWMTLRAYSMGMIANWGAHH
                     IDIAHWGLDLEETGPIRIRGRAEFPKRRLWDVHGSLDMRLTYANGTELHIADNSVYPN
                     GVRFIGEKGWIFCGRGSVKTLSNDPGAGGKHGRWRPLEASTKALIEGEVERPLPRNPG
                     NHHRVWLECIRTRQPAPILPESAHRTTTACILAFTAMNLKRGLKWDPVAERFVGDDEA
                     NATLARPERAPYGVRNALRRAGWASG"
     gene            complement(5742..6809)
                     /locus_tag="PK576_07240"
     CDS             complement(5742..6809)
                     /locus_tag="PK576_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61424.1"
                     /translation="MAEQDQTPQGAIPPKVTPFIKPNPEKPAVTPAATPAVTPAVTPA
                     ATPAITPAGAPRPITVRLKPVVVPGSPQAAVPAAGVPAPSPTPANLRATSRVLITEAM
                     LPDDAATPSNKDEEAPTSATTATVRPLATAASATVRLRPVTPPSLQPQPPPTPGSDPL
                     PSGPKPPSPAQVQASKSKTSRISLDSAIGVAPMTPMGAGKTEPKTIRLKRPVDLVPPA
                     ASGPKSSTTPIRQTSRIPDSALPSVADSAETASPTQKKTLKIKRPSVKPEGGETAAAA
                     GEAFPDGVQLTPISPLDFPPQEESTVLTTVAVVAGFAAALVLLLLVCCLGAHAVGPDA
                     GKNSVASLSGPELPWGGKLGK"
     gene            complement(6846..7400)
                     /gene="frr"
                     /locus_tag="PK576_07245"
     CDS             complement(6846..7400)
                     /gene="frr"
                     /locus_tag="PK576_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE61425.1"
                     /translation="MNDVLLDTVDKMDNTVNMLKDQFSGLRTGKASPALVDGVQVQYY
                     GTPTRLRDLASISTPEPRLIVINAYDPTALPEIEKAILAANLGVTPRNDGRVIRVPIP
                     ELNEERRKEICKVAKRQAEEARIAVRNVRREANDLVKALQKDGKITEDERDRGLEDVQ
                     KTTDAHIAKIDGELKNKEAEVMAV"
     gene            complement(7452..8159)
                     /gene="pyrH"
                     /locus_tag="PK576_07250"
     CDS             complement(7452..8159)
                     /gene="pyrH"
                     /locus_tag="PK576_07250"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE61426.1"
                     /translation="MLKLSGEVLGNKQTGECIDPDALASMARRVARIVELGVQIGIVL
                     GGGNIFRGLRGEAKGVNRTTGDNMGMLATIINGLAMQNALEQVGLDTRLQTAIQMHQV
                     AEPFIQRKAMRHLEKGRVVIFAGGTGNPYFSTDSAAALRASEIGASLLLKATKVDGIY
                     TADPNLDPNATRYDKVTYEDALLKRLKVMDSAAFALCMDNRIPIVVFDFYDEDALERI
                     VCGETIGTLVGDAAQIG"
     gene            complement(8366..10054)
                     /locus_tag="PK576_07255"
     CDS             complement(8366..10054)
                     /locus_tag="PK576_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE61427.1"
                     /translation="MFYERLPMNNNTLSCFTRSPRFSRLHVCVLTHFVLLPLLLSATE
                     YEYGPYTYIVTDNNEAAIIYYDGDNANVSIPSTLGGYPVAVIGDDVFEMCDSLKTVTI
                     PNSVKSIGNGAFCGCDALKSVTIGNSVTNIGDGVFSDCIALPSVIIPNSVKSIGNGAF
                     CGCEILKSVTIGSSVVTIGDGAFSACIALPSVIIPNSVTSIGDEAFSDCTALTSITIG
                     SSVASIGDSAFYGCESLTSVTIGNSVKSIGEYAFEDCIALKTIVMGNSVTNIGDEAFS
                     NCSSLTNITVSAGNPKYADRGGVLFDRSLTTLIQYPCGRSGSYTIPGDVTSIGAYAFS
                     WCAALTSVMISSSVTNIGDGAFSNCSSLTNITVSAGNPKYADRGGVLFDRSLTTLIQY
                     PCGRSGSYTIPGDVTSIGAYAFSWCAALTSVMISNGVANIGNRAFTQCGSLAVVLFTG
                     NAPTAGNLVFDGTPATLYYFPDTEGWSSSFANRPAVCWNPAFSSTSSASGMFAFTLTG
                     DADANIPVRIEASDSLTAPFWSAVTDTTIPASGTLNFTDADTRPSRFYRLTFPR"
     gene            complement(10079..>10412)
                     /locus_tag="PK576_07260"
     CDS             complement(10079..>10412)
                     /locus_tag="PK576_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE61428.1"
                     /translation="VAGMALTFARGMAERRGELALLNAAGLSRRVVLRLLLAEHGVTV
                     AAGLIAGILPALVAIRPAAQTLHSDFPWRTLTLLLFLLATTALLSIYFSIWVASRRIG
                     PDALKDEA"
BASE COUNT         2012 a         3004 c         3380 g         2016 t
ORIGIN      
        1 cgacctgtgc gtccaaggcc gtcttctcat cttatcccgt tctgtggcca ccgtttacgg
       61 tggcgcgaca cccctcaccc gcgaaacctg cctcgcacta aacgccgcca gcgctcacat
      121 tgcggaagcg gacggttgga tagcgcggtc cgtcaaatag aaaagacacc gtaggttttt
      181 ttgaagcggc gacatctgtc acttgtgggc ttgccttttc atggcaaaag agtactctgt
      241 cttttgtagt aaatcggtgg acggagggac cgttgcgccg gcgaaatcaa cagggaggga
      301 tcgtacatga agggttcagt tgcggtatca cgtcgcgcgt ttctagccgg tgcgggggct
      361 ctggcctcgg ttccatcgat cacgttgggg cagagtccgc gcgtatttaa gtatgcggtg
      421 gtcggatgtg gcggccgcgg cagaggtgcg gtgcgggata ttcaggcagc ggcggagcgg
      481 ctcggccaca aggcaaagct ggtcggcgcg gccgattttt tcaaggaaaa ggctgaggcg
      541 gtctgcaagt ccaacgactg cgacacgaaa ttcgcgttcg acggcgcggc cggataccgc
      601 aaggtcatgg agacggatgc cgaaatcgtg ctgttggccg ctcctccgat tttccgtccg
      661 ctgcaccttg aggcctgtgt ccgggccgga aagcacattt tcgcggagaa gccgattgcg
      721 acggatccgg ccggcctgcg ccatttcctg aaaggcgtgg aggaggccaa ggccaaaaag
      781 ctctcgatcc tgtcgggtac gctgcaccgc cattcgaacc ggtttctgcg gcagatcaaa
      841 ccgatccggg aaggcgcgat cgggcggatc cttgcgggcc aggtttaccg ctgccacggc
      901 ggcatttggg ttcgtccgcg ccgtgagggc gaatcgaacg ccggatacct ctgcaacaac
      961 tggtaccatt tctgggagat gtccggcgac cagatcacgg aacaggcgat tcatgagatc
     1021 gatctggcga actggttcat cggccgtttc cccaagaccg ccatggcgat cggcgcgcgt
     1081 caccgccggc ccgccgggat cgggaacatc tacgactgca tggcgatcga ttacgattac
     1141 ggcgacgccg tgcacgtgca ggcgatcggg cgccacatca agggctgctc ggatcgctgt
     1201 ggcacactgc tggtgggatc ggagggtgag gtcgcctgcg gcggcaaaat cacgcgcttc
     1261 gacggcaagc cggtcgccta tgacgaagag cgcatccggg gccgtcacga gaacggcatg
     1321 atcatggagc atgccgactt cctgcagggg attctcagcg gtgagttact gcatgaaggc
     1381 gaacaggtgg cgatgtcgac cgccacgacc atcatgggta cgctttcggc gtactcgggc
     1441 cggaccgtac ggatgtccga cctgttggag aacaaagagt ccgaattcta ctcgatgaat
     1501 tctgccttta cggccgcgga ctttgagcgc ggcacggata tcccgatgcc cgccgagggt
     1561 gttgcggccg tgccgggcaa agcctgagac cgcacgtgat tggatcacac atccgaccgc
     1621 ccggaccgga aacggaaacg caaccggttc gggcggcggg catagaccgt ggttcccggc
     1681 agtccgttgc cgagaaaagc gctgtggtcg catttggcgg acagcacctt cccagcccga
     1741 tcttgtgacc cggcaaggcg ggtcagtcga tccgcgtgac gacctgggcc ggaggcggcg
     1801 ggttgcggat ccatgctccg atctggcgca gggtccgcac atcgatctcg cgcagctttc
     1861 cggagtaatc cggcccgacg ttgatggaaa agagattacc gtatcgtgcc gcgccgagat
     1921 agtcccggta gagcttctcg ggcgtgtggc aaaggccgtc gtgttcgggc agcgagtaga
     1981 accaccttgc gccgcctttg tgtggaggca gaatcgggta ggtgaactcc gcgagccggt
     2041 agtttttgga aggggcgtta cgcatgtacg gggcggctgc agagtgatcg tccagcgacc
     2101 ctggcttgcc catttctcca agacggatat ctgctcccgt ctggtcgcca tggttaaacc
     2161 cgataaagca accgggctga agcgacttac agaaggcgag ggtctccgcg tgactcaggc
     2221 caccatcgcc gacggcgtgg tcgaaccaga tgtactcgat gggaccgtac tgcgtcagca
     2281 gttccttgag ttgcgctttt ttcatctccg gatcgcgacc gccctgattg tttttcttgg
     2341 gaccgggcgt ggcgggatca tccagaccgc cgggatggcg ccactcgcct tcggagaaat
     2401 agaacgcgag cttgatgccg tatttgtcgc aggatttttt gagttccgcc agcacatcgc
     2461 gtccgagcgg cgccttcgtg actttgcgat ccgtggtccg cgtgtcccac aggcagaagc
     2521 cgtcgtggtg cttggtgaga aagaggatgt agcccatctc cgcctctttc gccgtgcgcg
     2581 cccactggtc ggtgtccacc tcggtcgcgc ggaaaaacgc gaggtcctta atacccggcg
     2641 tccactcctt gccggagaag gtgctgaatg accagcaaat gaacaggccc cagcggaggc
     2701 ttttgagttc ttcggcacgc ctggtcatat cgaccggcgt atcggccacg acaaatgctg
     2761 aagacagaag tgtggccagt agtgcgagat gtgtgaatgt tcttttcacc gttctcccct
     2821 cttatcccaa aacggcccta ggctgctgcg cgcctcgacc gccacgggtg tttacttgca
     2881 ctactgaaag gatacaccct tctgctgaaa accgtccagc agaaacgacg cgggtttcgg
     2941 ggctctctcc tgtcttctct cccctccctg tgtttgcgtt ttactcaccg ggtctgatcc
     3001 gctatatttt ttcgtatgtt taacgcttac tctcccgaac tcaggcagga cctccgtttc
     3061 cgttggaacc ggttgcagga aaatctgcgc gaaaccgggg cggatgccct gctggccgct
     3121 acaaacgtca acctgctcta cctcacgggc cggatattca tggggtatgt ccacctcccc
     3181 gccgaaggcg agccgttgtt cttcgtgcgt cgcccctgcg gactttccgg cgcgaacgtt
     3241 tttgaactcc gtaagccgga acaaatcccg gacctgttgc gcgaggcggg gatcgccctg
     3301 ccgcggcacc tgctgctgga gggcgacgac ctgacattca acgaacacgc gcgcctgacc
     3361 gcctgcttcc cggacgcgcg catcggcaac ggttcaacgg atttacgcca atgccgcgcc
     3421 gtcaaaacgc cgtacgagat ccgaatcatg aggttgaccg gaagccgcca cgccgcggcc
     3481 gtggaccggt tcgcttcggt atacgaaccc ggcatgaccg atcaggcgtg gtcgatcgag
     3541 atgttccggc tgatgcttca ggccggaggc ctgggactgt tccgcatcgc gggcaacagc
     3601 atggaagggt tcatgggaac cgtgttggca ggcgacaacg gcggtgcggc ctcgccctat
     3661 gatttcgcgc tggggggcgc ggggctggac ccctcgcttc ccgtcggaca gagcggagtg
     3721 acgttgaccg aggggatgtc cgtgatggtg gacatcgccg gcaatttcta cggatacctc
     3781 acggattgca cgcgcacctt cgcgatcggc cgtctcgcgc cgcgcgcggt ggaggcgcat
     3841 tgccgttcga tcgagatcca gcaggcggtg gctgcggcgg gggtgccggg tgcgcggtgc
     3901 gaagacctct acgccctcgc gctcaaaatg gccgaagacg ccgggttcgc cgactgtttc
     3961 atggggcttg cgcagaaagc gaagttcatc ggacatggca cggggcttgt gatcaacgag
     4021 cagccggttc tcggcgcgcg ttcgaaagag acgcttcagg agggcatgtg catcgcgctg
     4081 gaacctaaat tcgtgatccc cggcaccggc gcggtcggtg tggaggacac gtttctcgtc
     4141 acgcccgccg gtatggagaa cctcaccccg tgcgacccct cgatgcgggt tttatgaggt
     4201 tgccgtaggc ggcggtgcag tgatatcatg ccacgtaaaa gtaagtgccg gaattcgtgg
     4261 gggaaagagg catggggcga aacggatttt cgagaagagg ttttcttgca gcgggggtgg
     4321 catgggggtt tcctgccatt attccgtcgc gcgtgctggg gagtcaggcg ccgtcgaaaa
     4381 agatacaggt gggggtgatc ggctgcggcc gcatcgcggg cgggatggac attcccggac
     4441 tgtggcacaa tcaagacctc gccgttccgg tcgcgctggc ggactgcgat atcaaacgtc
     4501 tgaacaccac ccgcgccaag actgcgcgac tctatgacgg agacctgccg gatctcaagc
     4561 tttatcagga ttaccgggcg ttgctggcgg acaagacggt cgatgcggtg atgatctgca
     4621 cgccggattt ctggcacgcg caacagtgcg tggaggcggc ctttgcgggc aaagacgtct
     4681 acgtgcagaa accgctggcc atgagcgtgt tcgagagccg caccattgtt gatgtgatgc
     4741 ggcgcacggg acgcgtcttc cacatcggta cgcagcaacg ctccgaaggg aagagcacct
     4801 tcgggccgca gttccgtaaa gccgcggaat atgtgcgcaa cggccgtctg ggacgtgtca
     4861 ggcgggtcga gatcggtttg ccgcaggaac ctgaggaacc ggccgactgg ccgctcgtgc
     4921 agccggtgcc ggatacgctg gattacgatc aatggctggg gtacacgccc gctgaactct
     4981 attccgaact gcgcacccat ccccagggca aaggcgcgga ggcggacttc ggcaggccgg
     5041 gctggatgac tctccgcgcg tacagcatgg ggatgatcgc caactggggc gcgcaccata
     5101 tcgatatcgc ccattggggg ctggatctgg aggaaaccgg accgatccgc atccgaggcc
     5161 gggcagaatt cccgaaacgt cggctctggg atgtccacgg ctcactcgac atgcgtctca
     5221 cgtatgcgaa cggaacagag ctgcacatcg cggacaacag cgtctatccc aacggtgtgc
     5281 gcttcatcgg cgagaagggt tggattttct gcggccgcgg ctcagtcaag actctgtcga
     5341 acgatccggg tgcgggcggg aagcacggac ggtggcgtcc gcttgaagcg agcacgaagg
     5401 ccctgatcga gggcgaggtg gaacgtccac tgccgcgcaa tccgggcaac caccaccgtg
     5461 tgtggctgga gtgcatccgg acgcggcaac cggccccgat cctgcctgaa tcggcgcacc
     5521 gcaccaccac ggcctgcatt ctggccttca cggccatgaa cctcaaacgc ggattgaagt
     5581 gggatccggt tgccgaacgt tttgtcggcg acgatgaggc caatgcgacc ctcgcgcgtc
     5641 cggagcgcgc gccctacgga gtacgcaacg ccctgcgtcg tgctggatgg gccagcggtt
     5701 gaatgcgcga agcaggggaa aagacgtttt ccccgtttcg cttacttgcc caattttccg
     5761 ccccacggca gctcggggcc gctgagggag gccaccgagt tcttgccggc atcggggcct
     5821 accgcgtgcg cgccgaggca gcagaccaga agcagcagca ccagggccgc ggcaaacccc
     5881 gccaccacag cgacggtcgt gagtaccgtg gactcctctt ggggcgggaa gtccagcggg
     5941 ctgatcggcg tcaactgcac gccatccggg aaagcctcgc cagccgcagc agcggtttcc
     6001 ccaccctccg gcttgacgct gggacgcttg atcttaagcg tcttcttctg cgtcggggag
     6061 gccgtttccg cagaatccgc cacactcggc agcgcggaat cagggatgcg ggacgtctga
     6121 cggatcggcg tggtagaact cttcggtccg gaggccgcag gcggcacaag atccacgggc
     6181 cgtttcaacc gaatggtttt cggctcggtc ttgccagccc ccatgggggt catcggcgca
     6241 accccgatgg cggaatccaa ggaaatacgc gaagtttttg atttggaggc ctgcacctgc
     6301 gctggggacg gcggcttggg gccgctcggc agggggtccg accccggagt cggcggcggt
     6361 tgcggctgca acgacggggg cgtcaccggg cgcagacgca cggtggcgga ggccgccgtg
     6421 gcaagcggtc ggaccgtggc tgttgtcgcg cttgtaggcg cctcctcatc cttgttggag
     6481 ggcgttgcgg cgtcatcggg cagcatggcc tcggtaatca gcacccgcga ggtcgcgcgc
     6541 agattggcag gtgtaggtga cggggcggga acaccggctg cgggcacggc cgcctgcggg
     6601 gaacctggga caaccaccgg cttgaggcgc acggtaatcg gacgcggcgc acccgcaggg
     6661 gtaatcgcgg gcgtggccgc gggggtaacc gcgggggtaa ccgcgggcgt ggccgcgggg
     6721 gtaaccgcag gtttttccgg attcggcttg ataaacgggg tcactttcgg gggtatcgca
     6781 ccttggggtg tttgatcctg ctcagccata actcctgcct ccgtttgggt tcagcgacag
     6841 ccgtatcaca cggccatcac ctccgcttcc ttgtttttca attcaccgtc gattttcgcg
     6901 atatgagcgt cggtggtttt ctgcacatcc tccagaccac gatcacgctc gtcctctgta
     6961 atcttgccgt ctttctggag cgccttgacc aggtcattgg cctcgcgccg cacgttgcgc
     7021 acggcgatcc gcgcctcttc cgcctgacgt ttggcgactt tgcagatctc tttgcggcgc
     7081 tcctcgttga gttccggaat cggcacgcgg atcacccggc cgtcattgcg cggcgtcacg
     7141 ccgaggttcg ccgccaagat ggccttctcg atttcaggca gggccgtggg atcgtacgcg
     7201 ttgatcacga tcagacgcgg ttcgggcgtg gagatgctcg ccagatcgcg caaccgcgtc
     7261 ggcgtaccgt aatactgcac ctgcacgccg tccaccaacg cgggagaggc tttgcccgtg
     7321 cgcagtcccg agaactggtc cttgagcatg ttgaccgtgt tatccatctt gtcgaccgtg
     7381 tcgagcaaca catcattcag cgtatccatg acattctcct gtccggcccg ccgttaacgg
     7441 gccgcttccg ttcagccgat ctgcgccgca tcgccgacca gtgtgccgat ggtctcgccg
     7501 cacacgatcc gctccaacgc atcctcatcg taaaaatcaa acaccacaat cggaatccgg
     7561 ttgtccatgc acaacgcgaa ggcggccgaa tccatcacct tcaaccgctt gagcagcgcg
     7621 tcctcataag tgactttgtc gtaccgtgtc gcgttcgggt ccagattcgg atccgccgtg
     7681 taaatcccat ccaccttcgt ggctttcagc agcagcgacg cgccgatctc actcgcgcgc
     7741 aacgcggccg ccgaatcggt gctgaaatac gggtttccgg tgccacccgc gaaaatcacc
     7801 acccggccct tttccaaatg acgcatcgcc ttgcgctgga tgaacggctc ggctacctgg
     7861 tgcatctgaa tcgccgtctg cagccgcgta tccaacccca cctgttccaa agcgttctgc
     7921 atggccaggc cgttgatgat cgtggcgagc atgcccatgt tgtcgccggt ggtccggttc
     7981 acacctttgg cttcgccccg caaccctcgg aaaatgttgc cgccgcccag aacgatcccg
     8041 atctgcacgc ccaactcaac gatccgcgcc acccggcggg ccatggacgc caacgcatcg
     8101 ggatcgatgc attcgcccgt ttgcttgttg cccagcacct cgccgctcag cttcagcaaa
     8161 atccgcttgt actggatttt accgctccgt cgcgtttcca gaaacgcccc ctttcagcac
     8221 cgtgatctaa acacagacag attaccgaaa ccctcctgaa agaacaaccg cttttttcgc
     8281 gtttcgcaac ccgcaaccca agaagacaga agagtggagg agaggaggcg ggaggcggga
     8341 acggaagggc aagcatttga ctttgttatc tgggaaaggt gaggcggtag aaacgcgagg
     8401 gccgggtatc ggcgtcagtg aagttgaggg tgccggaggc ggggatagtc gtgtcggtga
     8461 cagcggacca gaatggggcg gtgaggctgt cactggcctc gatgcggaca gggatgttgg
     8521 cgtcggcgtc gccggtgaga gtgaaggcga acatgccgga ggcggatgac gtcgaactga
     8581 aggcgggatt ccagcagacg gcgggacggt tggcgaagga ggatgaccag ccttcggtat
     8641 cgggaaaata gtagagcgtg gcgggagtcc cgtcgaaaac gaggttgccg gctgtcggag
     8701 cgttgccggt aaagaggacg acagcaaggg aaccgcactg ggtaaaggcc ctgttaccaa
     8761 tgttggcgac accgttgctg atcatgacgg aggtgagggc ggcgcaccag gagaaggcgt
     8821 atgcaccgat gctggtgacg tcaccgggaa tggtgtagga accggagcgg ccacagggat
     8881 attgaatcag cgtggttaaa ctacgatcga acaacacgcc gcctctgtcg gcatatttcg
     8941 ggttccctgc ggatacggtt atgtttgtaa gggaggagca attggaaaag gctccgtcgc
     9001 cgatgttggt gacactgctg ctgatcatga cggaggtgag ggcggcgcac caggagaagg
     9061 cgtatgcacc gatgctggtg acgtcaccgg gaatggtgta ggaaccggag cggccacagg
     9121 gatattgaat cagcgtggtt aaactacgat cgaacaacac gccgcctctg tcggcatatt
     9181 tcgggttccc tgcggatacg gttatgtttg taagggagga gcaattggaa aaggcttcgt
     9241 caccgatatt ggtgacgcta ttgcccatga cgatggtttt gagggcgatg cagtcttcga
     9301 aggcgtattc gccgatggat ttgacgctgt tgccgatcgt gacggatgtg agggattcac
     9361 agccgtagaa ggcactgtca ccgatagacg cgacactgct gccaatggtg atggatgtaa
     9421 gagcggtaca gtcggagaag gcttcatcac cgatggacgt aacgctgttg gggatgataa
     9481 cggaggggag tgcgatacag gcggagaagg ccccgtcgcc gatggtgacg acactgctgc
     9541 cgatggtgac ggatttgagg atctcgcacc cgcaaaaggc cccgttgcca atggatttga
     9601 cgctgttggg gatgataacg gaggggagtg caatgcagtc ggagaagact ccatcaccga
     9661 tgttggtgac gctattaccg atggtcacgg atttgagggc atcgcacccg caaaaggccc
     9721 cgttgccaat ggatttgacg ctgttgggaa tcgtgacggt cttgagggag tcgcacattt
     9781 cgaagacatc gtcaccgata acggcgacgg gataaccgcc gagggtggaa ggaatggaga
     9841 cgttggcatt gtcgccgtcg taatagatga tggcggcttc gttattgtcg gtgacgatgt
     9901 aggtgtaggg gccatattcg tattcggtgg cggataagag gaggggaagg agaacaaagt
     9961 gcgtgagcac gcagacatga aggcgcgaaa aacggggcga gcgtgtaaag caggataagg
    10021 tgttattatt catgggtaac ctttcataaa acataatttg aagactttcc gatttcggtc
    10081 acgcctcgtc cttgagtgcg tcagggccga tgcgtcgtga cgcaacccaa atggaaaagt
    10141 atatactcaa cagggcggtc gtcgcgagca gaaacagcag aagcgtgagt gtccgccagg
    10201 gaaaatcgct gtgcagcgtc tgggccgcgg gccggatcgc gaccagcgcg ggaaggattc
    10261 ccgctatcag acccgcggca accgttacac cgtgttcggc cagcagcaga cgcaacacca
    10321 cacgacggga gagcccggcc gcgttcaata aagccagttc accgcgccgt tccgccatgc
    10381 cccgcgcgaa agtcagcgcc atgcccgcga cg
//
LOCUS       DAOSLE010000092        10380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6284_length_10380_cov_9.356126, whole genome shotgun sequence.
ACCESSION   DAOSLE010000092 DAOSLE010000000
VERSION     DAOSLE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10380
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6284_length_10380_cov_9.356126"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1439
                     /locus_tag="PK576_07265"
     CDS             147..1439
                     /locus_tag="PK576_07265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HOE61429.1"
                     /translation="MSLLHRRAFLKSSLLATLAAQTKGMAAESPQTAPNSPAVSPILT
                     PPSLQIPAPTSMGIVWGVTTRATGFVDWATRPDFSDARRVFAGSTGLRTLDDAALGVR
                     LTGLAPDSVYHYRTGTVPIDFRGPYKILPGEPVLSDAYSFRTPGMRSPSSFAVINDTH
                     ENHKAFALLAAKLEELNPAVTVWNGDLCDWLDQMPKMLQTLLNPGKSAFAVSRPFLFT
                     PGNHDYRGLLARELPRLLLTREPTERASAYWTLERNYAYRQGEIALIGLDTGEDKPDG
                     HPAWGQLAQFTPYRELQAKWLADTLERPEIKSAPYLVAFCHIPLFDDSPKANPGDRLD
                     GWASWQRHCHDLWSPLFERYGLQLVITAHTHRFRFDAPTAKRSWAHMVGGAGCDVPPK
                     GQLTVLEGRVIGDRLKLTAHNLTDGSTLGEHTFAPRQV"
     gene            complement(1430..1897)
                     /locus_tag="PK576_07270"
     CDS             complement(1430..1897)
                     /locus_tag="PK576_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOE61430.1"
                     /translation="METTERLKIYRHLFRIGVPLIDRQHEQYLEWLDQLFTLTEQPSV
                     SRAAVNEAVAGAVAYAIEHFDAEEALMRSVAYPGYEGHVRKHDEFRSETDRLCADDPK
                     AVSPEERLFYLTKWLVNWFCDQTQVYDKALAVFLERQRSTCNGGSVGSEASQT"
     gene            complement(1914..3083)
                     /locus_tag="PK576_07275"
     CDS             complement(1914..3083)
                     /locus_tag="PK576_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOE61431.1"
                     /translation="MKAREICKGVQWMGCVDWNRRLFDALIPLPDGTSYNAYLVRGSE
                     KTVLFDAVDPAVSHVLLAQLADVPRLDYLVAHHVEQDHSGAIPAVLAKYPEAIVLCTP
                     KAKPLLTDHLAIAPERIRTVEDNETLPLGGKTLRFIHTPWVHWPETMCTYLPEDRILF
                     SCDFFGSHLATSALCSGTDPYVCEAAKRYYAEIMMPFRSMIRNNMKKVRELDVGLIAP
                     SHGPLHDHPEIMLSAYEDWSSDRVANQVVIPYMSMHGSTEMMVDHLVAALTARGVGVQ
                     PFELSTTDTGKLAMSLVDAATLVVGTPTVHVGPHPHVFAAVHLANALRPKVKYAAIIG
                     SYGWGTKAVEQVSALIPNLKVEMLGTLMCKGLPQADTFAALDALAENIRAKHEAL"
     gene            3314..3634
                     /locus_tag="PK576_07280"
     CDS             3314..3634
                     /locus_tag="PK576_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HOE61432.1"
                     /translation="MKKLLALAVLCVSSAMLTGCVFPMKGATTAALTLEHTVSDPIVD
                     NSVRAIKRGESYGGGILFFSTGDNSIDTAMRNGGITKVHHVNYKVMNILYLYNETVTI
                     VYGE"
     gene            3747..5120
                     /gene="purB"
                     /locus_tag="PK576_07285"
     CDS             3747..5120
                     /gene="purB"
                     /locus_tag="PK576_07285"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOE61433.1"
                     /translation="MDPFDPLTALSPLDGRYASKLEKLRPIFSETGLIRRRVSVEIAW
                     LEALCDAPGIPEALPLSDGERAALREIADKFSLEDARRVKAIEAITNHDVKAVEYFIK
                     ERLAGTTLASRGEFVHFACTSEDINNLAHALMLRDGLRVLRTAQERLTDALAAFARET
                     RDAAMLARTHGQPASPTTLGKELAVFVLRLRRQATQLDAVILPAKLNGAVGNYNAHLA
                     AYPDVDWPALARSVIEGRFGLRQNPLTIQIEPHDGMAELFDALARWNTVLLDLNRDIW
                     AYISLGYLGQKTVKGEVGSSTMPHKVNPIDFENSEGNLGLANAVLEHMARKLPVSRLQ
                     RDLTDSTVLRNMGVGFGYALTAYQATLKGLGKITLNAERLAADLDEAWEVLAEPVQTV
                     MRKLGQTNPYEQLKELTRGKQISAESMRGFIETLDLPEEDKRRLLALTPATYTGIASK
                     LVDAIQI"
     gene            5164..5769
                     /locus_tag="PK576_07290"
     CDS             5164..5769
                     /locus_tag="PK576_07290"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOE61434.1"
                     /translation="MNMEFLPSVSPEVFTWVVVPLLIFVARILDVSIGTLRVIFVSKG
                     YRLYAPILGFFEVVIWLLAIRQIMNHVDNVLCYLAYGLGFAVGNYIGIGLDERLSLGT
                     VLVRIVPRYDTTELIDHLRRMGFGASLVDIEGMSGKLKMIFAIVKRKDLTALLAIIQE
                     HNPQAFVTIEDVKTAKEGYFRPPQAKRAAPLSWLNALCNRK"
     gene            complement(5793..7145)
                     /locus_tag="PK576_07295"
     CDS             complement(5793..7145)
                     /locus_tag="PK576_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61435.1"
                     /translation="MKLTRRSFSQTSLAAILAAQSAPYLARGQAARRFRVVLIGAGWW
                     GTNLLHTALDSGVCDLAALCDVDERMLALCGEALAKRGVSRPKGYKDFRECLATEKPD
                     IALIATPDHWHALPMIEACRQGAHVYVEKPICHTILEGRAMVAAAARYGRVVQVGTHR
                     RASPHNQSAREFIRSGKLGPIGFIRCFVTYSGGPEKPKRNIEPPRGLDWDFWCGPAPL
                     RPFCGDLKNPWSSGIHPRGFRHFMDYANGQLGDWGIHWLDQVLWITGLRYPNTCHSVG
                     GRPIRGPAILTQEEQTTDAPDMQVVTWAFADGPTVTWEHRLFAGNPHERGENVGVYFH
                     GQKGVLHLGWQQGWTFYPSNRKEPVLHEDAQLNEPDGQNLRELFADFLDAIRTGRKPL
                     CDIESGHQATTMCLLGNLAMKHGRSVSWDGAKETTGEAAADALLRRPYRGAWTYPVAP
                     "
     gene            complement(7295..8662)
                     /locus_tag="PK576_07300"
     CDS             complement(7295..8662)
                     /locus_tag="PK576_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61436.1"
                     /translation="MMRQMFTRREALRVMGLGGAAILAGCRSPGGSRAVKGGAARWFR
                     GNLHMHTYWSDGRAFPEQAIALYKQLGYDFISVSDHNVFAEDRNVWRSVEDKGKGWPP
                     VVTRTLYDAYVKEFGSEVETRQENGKTWVRLKTHDEMVRRFGEPGRFLLMPAVEITQH
                     LSDRGIQVHMNYVNLPDVIPSVKGGPLNRRVDAERTEVRNVIRTSTEQVAQLAAKEKR
                     PYMLMLNHPQWIYWDIMPQHLIDNPAVRFFEVCNGGSAFAPHPDAPGLTNDSFWDAVN
                     AFRARDGAPLLYGVGSDDTHYYLNRTPEQRLADAWVMVRSAALTPEALFGAMHAGDFY
                     TSTGVTLEDVDFRPANRTLRVRVRPEPGVTYRIRFITTRKGFDPSVRTVEAPAVKGRG
                     ARTLPIYSADIGQTVSQVEGTTASYRMAPDDLYVRAKIESSVPSGYKRHFHPDVQVAW
                     TQPYR"
     gene            complement(8896..9900)
                     /gene="rnhC"
                     /locus_tag="PK576_07305"
     CDS             complement(8896..9900)
                     /gene="rnhC"
                     /locus_tag="PK576_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOE61437.1"
                     /translation="MEKKNSFTFTLNEEQQQALQIMLRMGNYRPKTVPHTIIAVEAQD
                     CVVNLYKSGKCLIQGKGAEDFVLFFLEPNVLQCATVGYEEILNPDLVAPHMGIDESGK
                     GDFFGPLVVCAVFVDPDLAHAMQELGVKDCKQLTDKAVFFIGNKTRQLLGPKRFAMVS
                     IGPEAYNRLYAKIRNVNSLLAWAHARCIENLLEAVPECPRAVADQFGAKQVIERALMK
                     KGRKIKLEQRHKAESDIAVAAASVLAREAFLRGLRRLGETYGIQVHKGASEQVKTSAI
                     ELVKKEGPEVLLKTCKCHFKTTDQVLAACGSSRAALGPEGQVVSRGKDSYSARGSGST
                     "
     gene            complement(9911..>10380)
                     /locus_tag="PK576_07310"
     CDS             complement(9911..>10380)
                     /locus_tag="PK576_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61438.1"
                     /translation="PLRIYVVHAALFKSPLEDGTTKLKNRPAFYGVSFIYANSPEAKG
                     KIGRLHQVWQDRRPPFFAKNGVPPDLGGGNGGIARLVSWRNGHETHSETGMTARAKTN
                     PANSHSPVLKTTLPITYIKVILISIPVRSAGGGLTSGTVIATNPPCEAKGILI"
BASE COUNT         1966 a         3382 c         3055 g         1977 t
ORIGIN      
        1 cgttcggaag aagtgaatgc gaccacccca gtcgcattta ctttgccaag tgaggctgcc
       61 atgcgtgccc gaaaaagaac attgccgcag aagaacggat tggggtatgc ttcaaaacat
      121 caggtttcaa aaagggagga tgagggatga gcttactgca ccgacgcgcg tttctaaaaa
      181 gttccctgct ggcgacactc gccgcgcaga cgaaggggat ggctgccgaa tccccgcaga
      241 cagccccgaa cagccccgcc gtctccccga tcctgacccc gccgtcgctg cagattccgg
      301 caccgacttc gatgggcatc gtttggggtg tcaccacccg cgccacgggt tttgtggatt
      361 gggccacgcg ccctgatttc tcggatgcgc gccgtgtgtt cgcgggcagc accggattgc
      421 ggactctgga tgacgcggcg ctcggcgtcc ggttgacggg ccttgccccg gattcggtct
      481 accactaccg caccggaacc gtgcccatcg acttccgcgg accttacaag atcctgcccg
      541 gcgagccggt cctgagtgat gcctactctt tccggacgcc ggggatgcgg tcaccctcct
      601 cgtttgcagt catcaacgac acgcacgaga accacaaggc cttcgcgttg ctggccgcta
      661 aactcgaaga gttgaatccg gccgtgacgg tctggaacgg cgatctttgc gactggctgg
      721 atcagatgcc caagatgctc cagaccctgc tgaatcccgg caagagcgcg ttcgccgtca
      781 gccgcccctt cctgttcact ccggggaacc acgactaccg cggtttgctc gcgcgggagc
      841 tgccgcggct tctcctgaca cgcgagccca ccgaacgcgc gagcgcgtac tggactttgg
      901 aacgcaatta cgcctaccgc caaggcgaga tcgccctgat cggtctcgac accggcgagg
      961 acaaaccgga cggacacccc gcctgggggc agctcgccca gttcacgccc taccgcgagc
     1021 ttcaggccaa gtggctggcc gacacgctgg agcgtcccga aataaaaagc gcgccctatc
     1081 tggtggcctt ttgccacatc ccgctgttcg atgactcgcc gaaggccaac cccggcgacc
     1141 gccttgacgg ctgggcctcg tggcaacgcc actgccacga cctgtggtcc ccgctctttg
     1201 agcgatacgg cctccaactg gtgatcacgg cccacacgca ccggttccgc ttcgacgcgc
     1261 cgaccgccaa acgttcctgg gcgcatatgg tgggaggcgc gggttgcgat gtgccgccca
     1321 aaggacagct cacggtcctt gagggacgcg tcatcggaga tcgcctgaaa ctcaccgcgc
     1381 acaatctgac cgacggctcc acgctcgggg agcacacctt tgccccgcgt caggtctgag
     1441 acgcctccga tccgacggat ccgccgttgc aggttgaacg ctgccgttcg agaaacaccg
     1501 ccagggcctt atcatagacc tgcgtctgat cgcagaacca gttgaccaac catttggtca
     1561 gataaaacag ccgctcctcc ggggacacgg cctttggatc gtcggcgcag agccgatccg
     1621 tctcactgcg gaactcgtca tgtttgcgca catgcccctc gtaccccgga taggcgacgg
     1681 aacgcatgag ggcctcttcc gcatcgaaat gctcgatggc gtaagccacc gctccggcga
     1741 ccgcctcatt gaccgccgcc cggctgacgg agggctgttc ggtcagggtg aagagttggt
     1801 cgagccactc cagatactgc tcgtgctgcc tgtcgatcag cggcacaccg atacgaaaca
     1861 gatggcggta aatcttcagt cgctccgtcg tttccacacg cacccccttc ccctcacagc
     1921 gcctcatgtt ttgcccggat attctcggcg agcgcatcca gcgccgcaaa ggtgtcggcc
     1981 tgcggcagtc ctttgcacat cagcgtgccg agcatctcga ctttgaggtt ggggatcagg
     2041 gccgaaacct gctcgaccgc tttggtgccc catccgtaag aaccgatgat cgcagcgtat
     2101 ttgaccttgg gccgcagcgc gttggccaga tgcacggccg cgaacacatg cggatgcggt
     2161 ccgacatgca cggtcggtgt gccgaccacc agagtggcgg catccaccag agacatggcc
     2221 agtttaccgg tgtcggtggt ggagagttcg aaaggctgca cccccacccc ccgcgcggtc
     2281 agggccgcca caagatgatc caccatcatc tcggtactgc cgtgcatgga catgtagggg
     2341 atcaccacct gattcgcgac gcggtcggag ctccagtcct catacgccga caacatgatt
     2401 tccggatggt catgcagcgg tccgtggctg ggcgcgatca gccccacatc cagttcccgc
     2461 acttttttca tgttgttacg gatcatcgac cggaacggca tcatgatttc agcgtagtag
     2521 cgtttcgccg cctcgcagac atacgggtcc gtgccagagc agagcgccga ggtcgccaga
     2581 tgcgagccga agaaatcgca ggagaaaaga atccggtcct caggcagata ggtgcacatc
     2641 gtctccggcc agtggaccca cggggtatgg atgaaccgca acgtcttccc gcccagcggc
     2701 agggtttcgt tgtcctccac cgtccggatg cgctccggcg cgattgccag atgatccgtc
     2761 agcaacggct tggccttggg tgtgcagagc acgatagcct cggggtattt cgccagcacg
     2821 gcgggaatcg cgccggagtg gtcctgttca acgtggtgcg cgaccagata atccaggcgc
     2881 ggcacgtccg ccaactgcgc gaggagcaca tgcgacacgg ccgggtccac cgcatcgaac
     2941 agcacggtct tttcgctgcc gcggaccaga taggcattat agctggtgcc gtccggtaaa
     3001 gggatcagcg cgtcgaacag acgccgattc caatcgacac accccatcca ctgaacacct
     3061 ttacagattt ctctcgcctt cattgctctc tccctctcgg gcgcgacccg gccgcacaaa
     3121 cctcgccggg cgccgtccga atgaccttgt gcccgtgtat gatgatggat tcggttggca
     3181 ctgtcaatcc gcggtgcgtc ggaggtttct catcgacggc ttgcaattgg ggtcggattt
     3241 atgcaacgat tgaacggatg cgaaacacgg caaggctcgc cgtgtcgcac ttcatcaaaa
     3301 aggagttatg aacatgaaga aattactcgc ccttgctgtg ttgtgcgtca gctctgccat
     3361 gctgaccggc tgtgtgtttc ccatgaaggg tgccactacc gcagcgctca cgctggagca
     3421 caccgtttcc gacccgattg tggacaacag cgtccgcgcc atcaagcgcg gtgagtccta
     3481 cggcggcggc atcctgttct tcagcaccgg cgacaactcg atcgacacgg cgatgcggaa
     3541 cggagggatc accaaggtcc atcacgtgaa ctacaaagtg atgaacatcc tgtatctcta
     3601 caacgagacc gtcaccatcg tgtacggcga ataaccgtgc cgccgggcat caggacccct
     3661 ttcttcggaa accgggctcc tgatgcccgc ccgtatcgct atgtccgcgc cgccggattt
     3721 gcctgcggcg ttttggagtc ctgcgcatgg atccgtttga cccgttgacc gccctgtcgc
     3781 cgctggacgg gcgctacgcc tcgaaactgg agaagcttcg ccccatcttt tccgaaaccg
     3841 gcttgatccg ccgccgcgtc agcgtcgaga tcgcctggct ggaggcgctg tgcgatgccc
     3901 ccggcattcc ggaggcactc cccctgtccg acggggagcg cgccgcgtta cgcgaaatcg
     3961 cggacaagtt cagcttggag gacgcgcgcc gcgtcaaagc catcgaggcg atcaccaacc
     4021 atgacgtcaa agcggtcgag tacttcatca aggaacggct cgccggcacc acgctcgcgt
     4081 cacgcggcga gttcgtccat ttcgcctgca cctcggaaga catcaacaat ctcgcgcacg
     4141 cgctgatgct gcgggacggg ttgcgcgtgt tgcgcaccgc ccaagagcgc ctgaccgacg
     4201 cgctggccgc cttcgcccgg gaaacgcgcg atgccgccat gctggcgcgc acccacggcc
     4261 agcctgcctc gcccaccacg ctggggaaag aactcgcggt ctttgtcctc cgcctccgcc
     4321 gacaggccac gcaactcgac gcggtcatcc tgccggccaa actgaacggc gcggtcggaa
     4381 attataacgc ccaccttgcc gcttatccgg atgtggactg gcccgccctg gcccgctcgg
     4441 tcatcgaagg ccgcttcggt ctgcgccaga atccgctcac gatccagatc gagccgcatg
     4501 acggcatggc cgagttgttc gacgcgctcg cgcgctggaa caccgtgctg ctggatctca
     4561 accgcgacat ctgggcctac atctcgctcg gttacctcgg gcagaagacc gtgaagggag
     4621 aggtcggctc ttcaaccatg ccgcacaagg tcaaccccat cgatttcgag aacagcgagg
     4681 gcaacctcgg cctggccaac gccgtgctgg agcacatggc ccgcaaactg ccggtctcgc
     4741 gcttgcaacg cgatctgacc gactcgaccg tactgcgcaa tatgggcgtg ggcttcggtt
     4801 acgcgctgac ggcctatcag gccacgctca aagggctcgg caagatcacg ctcaacgccg
     4861 aacggctggc cgcggatttg gatgaggcct gggaagtgct ggccgagccg gtccagaccg
     4921 tcatgcgcaa actcggccag acgaacccct atgagcaact caaagaactg acgcgcggca
     4981 aacagatctc cgccgagtcg atgcgcgggt tcatcgaaac actcgacctc ccggaagagg
     5041 acaaacggcg tctgctcgcg ctcacgccag ccacctacac cggaatcgcg tcgaagctcg
     5101 tcgacgcgat ccagatctga tgcgcggggg caacaaagcg tcccgcggaa aacgtgcgaa
     5161 caaatgaata tggaatttct cccctccgtc agtccggaag ttttcacatg ggttgtggtg
     5221 ccgctgttga tttttgtggc gcgcatcctg gatgtcagta tcggaacctt gcgcgtgatc
     5281 ttcgtctcga agggctaccg cctctatgcg ccgattctcg gattcttcga ggtggtgatc
     5341 tggctgctgg cgatccgcca gatcatgaat catgtggaca acgtcctgtg ctatctcgcc
     5401 tatgggttgg ggttcgcggt cggcaactac atcgggattg gcctcgatga gcgcctgtcg
     5461 ctcggcacgg tgctggtccg catcgttccg cgctatgaca ccacggaact gatcgaccat
     5521 ctgcgccgca tgggtttcgg cgcctcgctg gtcgatatcg agggcatgtc cggtaaactc
     5581 aagatgatct tcgccatcgt gaagcgtaag gatctgaccg cactgctggc gatcatccaa
     5641 gagcacaatc ctcaggcgtt cgtcaccatc gaggacgtca aaaccgccaa agaaggctat
     5701 ttcagaccgc cgcaggcgaa acgcgccgcg ccgttgtcgt ggctaaacgc cttgtgcaac
     5761 cgcaagtgac cccgcagggt ctgctttaaa aatcaaggcg cgaccggata agtccacgcg
     5821 ccgcggtacg gccgccgcaa cagcgcatcg gccgctgcct cgccagtggt ttctttcgca
     5881 ccgtcccacg acacgctccg accgtgtttc atggcgaggt tgccgagcag gcacattgtt
     5941 gtggcctgat gtccgctctc gatatcgcag agcggtttgc gccccgtgcg gatggcatcc
     6001 agaaaatccg cgaacagctc gcggaggttc tgaccgtccg gctcgttgag ttgcgcgtcc
     6061 tcgtgcagca cgggctcttt gcggttcgac ggatagaagg tccagccctg ttgccatccg
     6121 agatgcaaga cccccttttg gccgtgaaag taaaccccca cattctcgcc ccgctcgtgc
     6181 ggattccccg cgaaaagacg atgctcccac gtcacggtcg gaccgtccgc gaaggcccag
     6241 gtcacgacct gcatgtcggg cgcgtccgtg gtctgctcct cttgggtcag aatcgcggga
     6301 ccgcgaatcg gacgcccgcc gaccgagtga caggtgttcg gatatcgcaa cccggtgatc
     6361 cagagcacct gatccagcca gtggatgccc caatcgccga gctggccgtt ggcgtaatcc
     6421 atgaaatgcc ggaacccgcg cgggtggata ccgctgctcc aagggttttt gaggtcgccg
     6481 cagaaagggc gtaacggcgc gggaccgcac cagaaatccc agtccaatcc ccggggcggc
     6541 tcgatgttgc gctttggctt ctcgggtccg ccggaatagg tgacgaaaca acggatgaat
     6601 ccgatcggcc cgagttttcc ggaacggatg aattcgcgcg cgctttggtt atgcggcgag
     6661 gcgcggcggt gggtgccgac ctgcacgacc cgcccgtacc gcgcggcggc ggcgaccatg
     6721 gcacggccct cgaggattgt gtggcagatc ggtttctcca catagacatg tgcgccttgg
     6781 cggcacgcct cgatcatggg cagcgcgtgc caatggtcgg gtgtggcaat cagggcgata
     6841 tccggtttct cggtcgccag acactcgcgg aaatccttat agcctttcgg acggctcacg
     6901 ccccgtttcg ccaacgcctc gccgcacagc gccagcatgc gctcatccac atcgcagagc
     6961 gcggcgagat cacaaacccc gctgtcgagt gccgtatgga gcagattcgt gccccaccat
     7021 cccgcgccga tcagcacaac gcgaaaacgc cgcgccgctt ggccccgcgc cagatacgga
     7081 gcgctctggg ccgccagaat cgcggccagc gaggtctgcg aaaaacttct gcgagtcagc
     7141 ttcatgacgg tcctcccccc tgtctactca caccccaaag tcgtgctgaa gatggcatgg
     7201 cacgcccagt tccgcaggca gatcccgaac ttccccaacg gtttcgcttc cggcgttaca
     7261 ctgacgcgcc agatacgcgc acaacgcctt ttctttagcg gtaaggctgc gtccacgcta
     7321 cctgcacatc ggggtggaaa tggcgcttgt acccactggg gacactggac tcgatcttgg
     7381 cgcggacgta cagatcgtcc ggggccatgc gatacgaagc cgtcgtgccc tcaacctgac
     7441 tgacggtttg cccgatatcg gcagagtata tcggcaacgt gcgcgcaccg cggcctttca
     7501 ctgcgggggc ctccacggtc cgcacactcg gatcgaaccc ctttcgggta gtgatgaaac
     7561 ggatgcggta ggtcacgccc ggctccggac gaacccgaac acgcaacgtg cggttggcag
     7621 ggcggaaatc cacatcttcc agcgtcacgc cggtggaggt gtaaaagtcg cccgcatgca
     7681 tcgcgccaaa gagcgcctcg ggtgtcagtg ccgcgctacg gaccataacc caggcgtccg
     7741 ccagacgctg ttccggcgta cggttcagat agtagtgggt atcgtcggaa cccacgccgt
     7801 aaagcagcgg tgcgccgtcg cgcgcccgga acgcgttgac cgcatcccag aacgagtcgt
     7861 tggtcaaccc cggcgcgtcc ggatgcggcg caaaggcgga accgccgttg cagacttcga
     7921 agaaacgcac cgccggattg tcgatcaggt gctggggcat gatatcccaa tagatccact
     7981 gcgggtgatt gagcatcaac atgtagggcc gcttctcttt tgcagccaac tgcgcgacct
     8041 gttccgtact cgtgcggatc acattccgca cctccgtccg ttccgcatcc acacgcctgt
     8101 tcagcggacc gcccttaacg ctggggatca cgtcgggaag gttcacgtaa ttcatatgca
     8161 cctggatccc gcggtctgac aggtgctgtg tgatctccac cgcaggcatc agcaggaacc
     8221 ggcccggctc cccgaaccgc cgcaccatct cgtcgtgggt cttgagccgt acccaggtct
     8281 tcccgttctc ctggcgcgtc tcgacctcac tcccgaactc ctttacgtac gcgtcataga
     8341 gagtccgggt taccaccggc ggccaccctt ttcccttgtc ctcgaccgaa cgccagacgt
     8401 tgcggtcctc cgcgaacaca ttgtggtcgc tgacgctgat gaaatcgtat ccgagctgct
     8461 tgtacagggc aatcgcctgt tccggaaagg cccggccgtc agaccaatag gtgtgcatgt
     8521 gcaggtttcc gcggaaccac cgcgccgcac cgcctttcac cgcgcggctg ccaccgggcg
     8581 aacggcaacc ggccagaatc gccgctcctc cgagccccat tacccgcaac gcctcccggc
     8641 gcgtgaacat ctgtcgcatc atgtcctcct cgaattaact cttacggggc gagtataacg
     8701 cagatcgccc ccccggacaa ggcggtcgtt gcgtcagggc tcggagagtt attcctcaac
     8761 cgtgccgtgg cgaatgcctt ggggcagctc agcagaaatc gcgctcagcg cataaccggg
     8821 catcaggctc aacaaaagaa gacggtgcct catttgacct ccttgaccat tcccttcaaa
     8881 ttggatagcg gaacattacg tcgagccgct gccgcgggcg gagtaggagt ccttgccgcg
     8941 cgaaaccacc tgaccctcgg gaccaagcgc ggcgcgcgaa gatccgcagg cagccagcac
     9001 ctggtccgtg gttttaaagt ggcatttaca ggttttcaga agcacctcgg ggccttcttt
     9061 cttcaccagc tcgatggcag aggtcttgac ctgctcggac gcgcccttgt gcacctgaat
     9121 gccgtacgtc tcccccaacc ttctgagtcc ccgcaaaaag gcctcgcgcg ccagcaccga
     9181 cgccgccgcg accgcaatat ccgactcggc cttgtggcgt tgctccagtt tgattttgcg
     9241 cccttttttc atcagcgcgc gctcgatcac ctgtttcgca ccgaactggt ccgccacagc
     9301 acgcggacac tccggcacgg cctccagcag gttttcgatg caacgcgcgt gagcccaagc
     9361 cagtagcgag ttgacgttgc ggatcttggc gtagagccgg ttgtaggcct cgggaccgat
     9421 cgataccatc gcgaaccgtt tcgggccgag cagctgccgc gttttgttcc cgatgaaaaa
     9481 gaccgccttg tccgtaagct gtttgcagtc tttgacgccg agttcctgca tggcgtgggc
     9541 gagatcggga tccacaaaca ccgcgcacac cacgagcgga ccgaagaagt cgcctttgcc
     9601 gctctcgtcg atgcccatat gcggggccac gaggtccgga ttcagaatct cctcataccc
     9661 cacggttgcg cactgcagca cgttgggctc cagaaaaaag aggacgaaat cctccgcgcc
     9721 cttcccctgt atcaggcact tgccgctttt atacagattc accacgcaat cctgcgcctc
     9781 gaccgcaatg atcgtgtgcg gcaccgtctt gggccggtag ttgcccatcc gaagcatgat
     9841 ctggagtgcc tgctgctgct cttcgttgag cgtgaacgtg aatgaattct ttttttccat
     9901 cgcaccgaca ttagatcaaa atcccctttg cctcacaagg tggattggtg gcgatgacgg
     9961 ttccggatgt tagcccgccg cccgcgcttc gcacgggaat ggaaatgagg ataactttga
    10021 tgtaagttat aggcaaggtg gttttcaaaa ccgggctgtg ggagttggcg gggttggttt
    10081 ttgcgcgggc ggtcatgccg gtctcgctgt gcgtctcgtg cccgttgcgc cacgacacga
    10141 gccgggcgat gccgccgttg cccccgccga ggtccggggg cacgccgttt ttcgcgaaga
    10201 acggcgggcg cctgtcctgc caaacctggt ggaggcgtcc gatcttcccc ttggcctcgg
    10261 gggagttggc gtagatgaag gagacaccgt agaaggcggg gcggttcttg agtttcgtgg
    10321 tgccgtcctc aagcggggac ttgaagagcg ccgcgtggac gacgtagatc cggagcggca
//
LOCUS       DAOSLE010000093        10260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6360_length_10260_cov_7.407741, whole genome shotgun sequence.
ACCESSION   DAOSLE010000093 DAOSLE010000000
VERSION     DAOSLE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_10260_cov_7.407741"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..2276
                     /locus_tag="PK576_07315"
     CDS             105..2276
                     /locus_tag="PK576_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HOE61439.1"
                     /translation="MSITRQKAIESAVQHRVSDEPVLSREPVQACFGSHVFGEDPMRE
                     RLPKDVYQVLRQTIEQGMPLDIRVADVVAAAMSRWAIERGATHYAHVFYPLTGLTAEK
                     HDSFFNPDGKGHLLVDFSGKQLIQGEPDASSFPNGGIRATFEARGYTAWDVTSPAYLM
                     EGRNGITLCIPTAFCSWTGEALDKKVPLLRSMQAVHRQAARVLKLFGHDDPGMINATA
                     GPEQEYFLIDRSLYYARPDLLSAGRTLFGTRPPKGQELEDQYFGAIPARVLAFMTEVD
                     AELYRLGVPVKTRHNEVAPAQYEIAPVFESANLATDHQYLIMQTLQAVAPKYGLACLL
                     HEKPFAGINGSGKHLNWSLNGPKVGNLLNPGETPHENMQFLVFCAAVIRAVDLHAVML
                     RSSVAYAGNDHRLGATEAPPAILSVFLGEQLTDVFEQIAAGGACQSKKAGTLTVGVDT
                     LPQLPRDVTDRNRTSPFAFNGNRFEFRAVGSSQSIAGPQVILNTIVADSLDYIATELE
                     KAPSEPAKFNAAVQKLLQKIIREHGRIIFNGDNYSAEWREEALRRGLPDARTTPEAID
                     AMDVPATYEVFEKCGVLNRREAESRFEIYRRKYIKDVLIEARLCLSMARTMIFPAGVR
                     YQRLLAQTAADLQALGKTTCTTVLDEVNALLAELQQAVRRLEETIEIPPGLNLSQQGR
                     YLVDTVIPLMNALRKVVDELEQICDDGLWPLPTYQEMLFIR"
     gene            2319..4259
                     /locus_tag="PK576_07320"
     CDS             2319..4259
                     /locus_tag="PK576_07320"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOE61440.1"
                     /translation="MLKRFGYCRVCASSPRVVPGAVEANVAQMVEEARRADAAGVDVL
                     VFPELCVTGYTCADLFFQDTLLDAVERGLAGFLEATRTLAPLCLLGLPVRVSCGVCNA
                     AAVCCAGRLLGVVPKTHLPNTREFSERRWFLSGTGLPDCSLRLCGDEIPFGTDLLFQH
                     EENRLLCVGAEICEDLWSPLPPSGALTLNGATVICNLAAGNELVGKAAYRRALVLQQS
                     ARCHAAYVYAGAGPGESTTDLVFGGHTLIAENGSLLAEGERFERDGTRTLADADLAFL
                     AFERRQNRAFDACAAGVAPVRRVGFVMPERARPVTRRLLRPIAAHPFVPADASERDAR
                     CAEVFALQANGLATRLERAGLRRAVVGLSGGLDSALALLAVCEAFDRVGWERSGILAL
                     TLPGFGTSRRTLDNALALGRALGVTVETIPIAESCKRHLAEIGHTGEPVDTTYENAQA
                     RERTQILMDRANMLQALVVGTGDLSELALGWSTYNGDHMSMYGINAGVPKTLVRDLIR
                     WVAEHRADASVAPVLSDILDTPVSPELLPADAAGSITQKTEDVLGPYEMHDFFLYQMV
                     RCGASPEKIRFLAAQAFEGRYGEEEISRALDLFLRRFFSQQFKRSCLPDGPKVGSINL
                     SPRGDWRMPSDASPESWLVKTH"
     gene            4364..6283
                     /locus_tag="PK576_07325"
     CDS             4364..6283
                     /locus_tag="PK576_07325"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOE61441.1"
                     /translation="MKPARKSKQNTRSVIPPVKPRIRITDTTLRDAHQSLWATRMRTD
                     DILAIAEEVDNAGYYSLECWGGATFDVCLRFLRENPWERLRQLKAVCKKTPLQMLLRG
                     QNILGYKNYPDDVLERFIALACENGMDIFRIFDALNDTRNLEKAIRTVKQYGGHAQGT
                     ICYTYSPVHTVENFVAIAKEQEAMGIDTLCIKDMAGILTPAAATDLIGALVKAVGVPV
                     QVHSHMTSGMAVAMYLSAVKAGAGAIDTAVSTMSGFSSQPPTETLMAILEAEGYDTGL
                     DRALVAKSNAHFRMLKAVREPASSGQMESVDAEVLAHHIPGGMISNLRSQLQQQGALD
                     RLPEVLEELPRTRKDMGYPPLVTPTSQIVGVQAVLNVLAGKRYEMVTQETRDYVRGLY
                     GRSPAPIDTRLASKILNGEKRVTCRPADLLNPGLAEAAAQIDPRLIEKEEDILSYCLF
                     PEVALGYFAWRAAPPDQRDPIPADLEASDKKQTASAAVPEAEPDPVSAPAEETLAMAE
                     IGRLVADLAGRLGIAAGSLSGLTLPAPVAKAAPAAVKPASAPKSAKPEAPAVAGPTIN
                     APLNGTFYRSPGPGKPELAKEGDSLQTGDSICIVEAMKLFNQIKADKPLKVVKFLVSH
                     GTVVAKGQPLVSVQY"
     gene            complement(6331..8100)
                     /locus_tag="PK576_07330"
     CDS             complement(6331..8100)
                     /locus_tag="PK576_07330"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE61442.1"
                     /translation="MKRHHIAPVMIGMGLILVVGLSALPARTQTRDPSAPAQPVSETA
                     SGVLATGKKSVQAVGEATSELGDSLCSGGRQAVDQSRKLWQDVLLPAMQRFAAALPGV
                     LKAVILLLAFWLVGKFAGACVTRLLNLTRIDERAVRDWGLESLLKRPTGETCSLASLV
                     GNIVKWLLVLFGFVAFFNALNLSMVAGPLQNLLDRIIGVIPNLIKAVAILLVYWILAA
                     VVRAGVTRALGALKFDERAGKHLSLPTTDESQPLGPGAMIGRLLFYVILLFGIPPFLQ
                     ALDQQALVAPLQDMLSKALGILPNLFAAALILVVGRVVTAIVREVVTNFLAASGADAA
                     AEKFGLSRTLGGNRLSAIAAKVVSFFILIPILISAVDCLGIRAISDPLKAMLQTILDA
                     VPALLVAAIIVLIGYVVAKVVRALVESFLSGIGTDELPGRLGLTFLEARAGQTPPSKL
                     IGAITAVVILLITAQQACAKLRFDQLAELLQRIVAYLPNLAVGLIILLAAMSLGKRFG
                     TMVAGALENHANGKALSVVAHYAVLLLGLSMALEQLGVGKEIVMIAVGSLLGGSALAL
                     GLAFGLGGQDRARELIERWKTGK"
     gene            8405..9451
                     /locus_tag="PK576_07335"
     CDS             8405..9451
                     /locus_tag="PK576_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61443.1"
                     /translation="MSSPRITKEARASWLRLYETVDRIDRLSPWPWMEPASCFGVAVP
                     DMEEPCFVLFGEPGREMRAIRILLGWKAFYEMMTRLADPAKQGVAWLLEIRMIEVLCA
                     PEPLLFQHERDFLRAIRREPDGKGEWTIFRSIVPGYHPWLPDAKERKLLEDVLYQAFG
                     MALRVEADPGMLRSRFPREILIRTSDGKGGWRDVWSRVKELSGEEIEVRIESRRLQAL
                     AAKPTCPVTLQVDLLFTPFSIQPGRERPQTAYVLLAVDAQSGLIHCGELFQAIGGIAA
                     MWSEIPDRLIALFERLGGCPDAIEVRGDRMANLMRPLTEFLPFKLVRREHLKMLDEAR
                     VGLSAYLRNGEEEA"
     gene            complement(9414..10088)
                     /locus_tag="PK576_07340"
     CDS             complement(9414..10088)
                     /locus_tag="PK576_07340"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE61444.1"
                     /translation="MPNLPYRTLSLLTLVLLAVILPFFLFADAVQAWTDALLRHAQAH
                     PLQTGLLLTALLASDIVVPVPSSLVSTACGMTLGFVAGTCASFIGMSASALVGLLMGR
                     MAAPAAQRLIGADEVRLLQSFHARYGIWLLLALRPVPVLAEASVVFSGLARQPFGRAF
                     AITLLGNAAVSAVYAAVGAWGRLADSFLPAFGASMLLAGALYLPLRRRGQHRATLPPP
                     RSADTR"
BASE COUNT         1934 a         3070 c         3380 g         1876 t
ORIGIN      
        1 aacagtccaa tatgttgtgc ttgcgcaaaa tggaggttgg cgtggcgtct gtttgaaggg
       61 ccgtgtcggt tcttcggtca cgacacgtca aggcggagaa ggcgatgagc attacgcgac
      121 agaaagcgat tgagtcagcg gttcagcacc gggtcagcga cgagccggtt ttgtcacgcg
      181 agccggtgca ggcctgtttc ggctcgcatg tattcggtga agatccgatg cgggagcgac
      241 tgcccaagga cgtgtatcag gttttgcggc agacgattga gcaggggatg ccgctggaca
      301 tccgcgtcgc ggatgtggtg gccgcggcca tgagccgctg ggccattgag cgcggtgcca
      361 cgcattacgc gcatgtgttt tatcctctga cggggctgac cgccgagaag catgacagct
      421 ttttcaatcc ggatggcaag gggcatctgc tggtcgattt cagtggcaaa cagttgattc
      481 agggcgagcc ggatgcgtcg agcttcccca acggcgggat ccgcgccacc tttgaggcgc
      541 gcgggtatac cgcttgggat gtgaccagcc ccgcgtattt gatggagggc cgcaacggca
      601 tcaccctctg cattccgacc gccttctgct catggaccgg tgaggcgctc gacaaaaaag
      661 tgccgctgct gcgttcgatg caggctgtcc accgccaagc ggcgcgtgtc ctcaaattgt
      721 tcggccatga cgaccctggc atgattaacg caacggctgg tccggaacag gagtacttcc
      781 tgattgaccg cagcctttac tatgccaggc cggacttgct gagtgccggg cgcaccctgt
      841 tcggaacccg tccgcccaaa ggtcaggaac tggaggatca gtatttcggt gccatcccgg
      901 cgcgtgtgct ggccttcatg accgaggtcg acgcggagtt ataccggctt ggggttccgg
      961 tcaagacgcg tcacaatgag gtcgcgcccg cgcagtatga gattgcgccg gtgttcgaga
     1021 gcgccaacct cgcgaccgat caccagtacc tgatcatgca gacgctgcag gcggttgccc
     1081 ctaaatacgg gttggcctgt ctgctccacg agaagccctt cgcggggatc aacggttcgg
     1141 gtaagcacct caactggtcg ctgaacgggc ccaaggtggg taatctgctc aatccgggcg
     1201 agacgccgca cgagaacatg cagttcctgg ttttctgcgc cgccgtgatc cgcgcggtcg
     1261 atctgcatgc ggtcatgttg cgcagctctg tagcgtacgc cggaaacgac caccggctgg
     1321 gcgcgaccga agccccgccc gcgatcctct ccgtttttct gggtgaacag ctcacggatg
     1381 tcttcgagca aatcgcggct ggcggcgcgt gtcagtccaa gaaagccgga accctgaccg
     1441 tgggtgtgga cacgctgccg caattgccca gggacgtcac ggaccgcaac cgcaccagtc
     1501 cttttgcgtt caacggcaac cggttcgaat tccgtgccgt gggctccagc cagtcgatcg
     1561 ccggaccgca ggtgatcctc aacacgatcg tggcggattc gcttgattac atcgccaccg
     1621 aactggaaaa ggcacccagc gagcccgcga agttcaatgc cgcagtgcag aagctgctgc
     1681 agaaaatcat acgcgagcac ggtcggatca tctttaatgg cgacaactac tcggcggagt
     1741 ggcgtgaaga ggcgctccgg cgcgggttgc ccgatgcccg tacaaccccc gaggcgatcg
     1801 atgcgatgga cgtgccggcc acctatgagg tgttcgaaaa atgcggtgtg ctcaatcgcc
     1861 gcgaggctga gagccgtttc gagatctacc gcaggaagta catcaaggat gtgctgatcg
     1921 aggcgcgcct ctgcctctcc atggcgcgaa ccatgatctt cccggcaggg gtgcggtatc
     1981 agcggctgct ggcgcagacg gcggccgatc tgcaggcgct cggaaaaacg acctgcacga
     2041 ccgtgctgga tgaggtgaac gcgttgcttg ccgaattgca gcaggcggtg cgccgcctgg
     2101 aggagacgat cgagattcct ccggggctga acctctcgca gcagggacgc tatctggtgg
     2161 atacggtcat cccgctgatg aacgcgctgc gtaaggtagt ggacgagttg gagcagattt
     2221 gcgatgatgg gctctggcca ttacccacct atcaagagat gctgttcatc cgttgaggcg
     2281 gaaccgggtt ccctcaggcg gatgaggggg gagcgacgat gcttaaacgt ttcggatatt
     2341 gccgggtctg cgcgtcaagc ccgagggttg tgcccggcgc ggtggaggcg aacgtcgcgc
     2401 agatggtgga ggaggcccgc cgagcggacg cggcgggtgt ggacgtgctg gtcttccccg
     2461 agttgtgcgt gacggggtac acgtgcgcgg acctcttctt tcaggacacg ctgttggatg
     2521 cggtcgaacg cggactggcg gggtttctgg aggcgacgcg gacgttggcg ccgctatgtc
     2581 tgctcggtct gccggtgcgt gtgtcttgcg gcgtctgcaa tgccgccgca gtctgttgtg
     2641 cggggcgtct gctgggcgtg gtgccgaaaa cgcatctgcc caacacgcgc gaattcagtg
     2701 agcggcgctg gtttctgtcg ggcaccggac ttccggattg ttcgctccgg ctttgtggtg
     2761 acgagatccc gtttgggacg gacctgctgt ttcaacacga ggaaaaccgt ctgttgtgtg
     2821 tcggggcaga gatctgcgaa gatctatgga gtccgctgcc gcccagcggg gctttgacgc
     2881 tgaacggcgc cacggtgatc tgcaatctcg cggccggcaa tgagttggtc ggcaaggccg
     2941 cataccggcg tgcgctggtg ctccagcagt ccgcgcgctg ccacgccgca tacgtgtacg
     3001 ccggggcggg ccccggtgaa tccacgacgg atctggtctt cggcggacac acgctgatcg
     3061 ccgaaaacgg cagtctgctc gcggagggcg agcgtttcga gcgcgatggc acgaggacgc
     3121 tggcagatgc cgatctcgcg ttcctcgcat tcgagcgtcg gcagaaccgc gcctttgacg
     3181 cctgtgcggc aggggtcgcg ccggtccggc gtgtgggttt cgtcatgcct gaacgcgcga
     3241 ggcctgtgac ccgacgcttg ctgaggccga ttgccgcgca cccctttgtc ccggcggatg
     3301 cgtcggagcg ggacgcgcgg tgcgccgagg ttttcgcgct gcaggccaac ggtttggcca
     3361 cgcggctgga gcgcgccgga ttgcggcgcg cggtggtggg gctctccgga ggattggatt
     3421 ccgcgctggc gctgctggca gtgtgcgagg cgttcgatcg cgtcggttgg gaacgctccg
     3481 gtattctggc gctgaccctg ccgggcttcg gaacatcccg ccgcacgttg gacaacgctt
     3541 tggcgctcgg tcgcgcactg ggggtcacgg tggagacgat cccgatcgcg gagtcctgta
     3601 aacggcatct ggcagaaatc ggccacaccg gcgaaccggt ggacacgacg tatgagaacg
     3661 cgcaggcgcg cgagcggacc cagattctga tggaccgcgc caacatgctg caagcgctgg
     3721 tggtcggcac cggtgatctt tccgagctgg cattgggatg gtccacctac aatggggacc
     3781 atatgagtat gtacggcatc aacgccggcg tgcccaaaac cttggtgcgc gatttgatcc
     3841 gttgggtggc ggagcatcgg gccgacgcgt cggttgcccc ggtgctgtcc gacattctgg
     3901 acacccccgt ttcgccggaa ctgctgcccg ccgatgccgc gggaagcatc acgcaaaaaa
     3961 cggaggacgt cctgggcccc tacgagatgc atgacttctt cctctaccag atggtgcgtt
     4021 gcggcgcatc gccggagaag atccgctttc tggccgcaca ggcgttcgag ggccggtacg
     4081 gggaagagga gatcagccgt gcgctggatc tgttcctgcg gcgttttttc agccagcagt
     4141 tcaaacgctc ctgcctgccg gacgggccca aggtcgggtc catcaacctt tcgccgcgtg
     4201 gtgactggcg tatgcccagc gacgcctccc cggaaagttg gctcgttaaa acacattaac
     4261 aatcttcggg gtgtgggggg ggattcccga aaatcgttcg tgacaaaatg acgggaaatg
     4321 tcagaatgaa aggcattttt ctttcagagg agaaaaggtc tctatgaaac ctgcgcgcaa
     4381 gagcaagcaa aacacgcgga gcgtaatccc tccggtcaag ccccgcatcc gcatcacgga
     4441 caccacgctg cgtgacgccc accagtccct ctgggccacg cgcatgcgta cggatgacat
     4501 tctcgcgatc gccgaggagg tggacaacgc cgggtactat tcgctggagt gctggggcgg
     4561 ggcgacgttc gacgtgtgcc tgcgttttct gcgcgagaat ccttgggagc gtctgcgcca
     4621 gctgaaggcg gtctgtaaaa agacgccgct gcagatgctg ctgcgcggac agaatatttt
     4681 gggatacaaa aactatccgg acgatgtgct ggagcggttc atcgcgctcg catgcgagaa
     4741 cggcatggat atcttccgca ttttcgacgc gctgaatgac acgcgcaatc tcgaaaaagc
     4801 gatccgcacc gtcaagcaat acggcgggca cgcgcagggt acgatctgtt acacctacag
     4861 tccggtgcac acggtcgaaa atttcgtggc catcgccaaa gagcaggagg ccatgggtat
     4921 cgacaccctg tgcatcaagg acatggcggg catcctcact ccggccgcgg ctacggatct
     4981 catcggggcg ctcgtcaaag cggtcggcgt tccggttcag gtgcactccc acatgaccag
     5041 cggcatggcg gtcgccatgt atctctccgc agtcaaggcc ggtgccgggg cgatcgacac
     5101 agcggtttcg accatgtcgg ggttctcctc tcagccgccg acagagacgc tgatggcgat
     5161 tctggaggcg gaaggttacg ataccgggct tgatcgcgct ttggtggcca agagcaacgc
     5221 ccatttccgg atgctgaagg cggtgcgtga acccgcctcc tccggtcaga tggaatcggt
     5281 ggatgcggag gtgctggcgc atcacatccc cggcgggatg atctccaacc tgcgctcgca
     5341 gttgcagcag cagggcgcgc tggaccggtt gcccgaagtg ttggaggagt tgccgcggac
     5401 gcgcaaagac atggggtatc cgccgcttgt cacgccaacc agccagattg tgggcgtgca
     5461 ggctgtgttg aatgtgctcg ccggaaaacg ctatgagatg gtcacgcagg agaccaggga
     5521 ttacgtccgg ggcctttacg gccgttcgcc cgccccgatc gacacccgtc tggcaagcaa
     5581 aatcctgaac ggcgagaagc gtgtgacgtg ccgtcctgcg gatctgctca accccggctt
     5641 ggctgaagcc gcggcgcaga tcgatccgcg cctgatcgag aaggaggagg atatcctttc
     5701 gtactgcctc ttccccgagg tggcgctggg ctacttcgcg tggcgcgccg cgccgcctga
     5761 tcagcgcgac ccgattcccg cagatctcga ggcttcggat aagaagcaga ccgcgtcggc
     5821 cgcggttccg gaagcggagc ccgatcccgt gtccgcgcct gcggaggaga ctctggccat
     5881 ggccgagatc ggcaggttgg tggcggatct cgccgggcgg ctgggaatcg ccgctgggag
     5941 tttgtccggt ctgacccttc ccgcgccggt tgcgaaagcc gcgcctgccg cggtcaaacc
     6001 cgcgtccgcg ccgaagtccg cgaaacccga agcgcccgcc gttgcgggac cgacaatcaa
     6061 cgcgccgctg aacggaacgt tttaccgctc gccgggtccg ggaaaacccg aactcgccaa
     6121 agagggcgat tcgctccaga ccggtgattc gatctgcatt gtcgaggcca tgaagctctt
     6181 caaccagatt aaagccgaca aaccgttgaa ggtggtcaag tttctggtgt cgcacggtac
     6241 ggtggtggcc aaaggccagc cgttggtgtc cgtccaatac tgatccgctt cggacaaaaa
     6301 aagggcgggg ggttccccgc cctttttcca ttactttccg gttttccagc gttcgatcag
     6361 ttcgcgcgcc cggtcttggc ccccgagtcc gaaggccagt cccagagcga gcgcactgcc
     6421 gcccagcagg gatcccactg cgatcatcac gatctccttg cccacgccga gttgctccag
     6481 cgccatgctg aggccgagga ggagcacggc gtagtgcgcg acgacggaga gcgccttgcc
     6541 gttcgcgtgg ttctccagcg cgcccgccac catggtgccg aaacgcttgc cgaggctcat
     6601 ggccgcaagc aggatgatca gccccaccgc cagattcggc aggtaggcga cgatccgctg
     6661 caacagttcg gccaactgat cgaaacgcaa cttggcacag gcctgctgcg ccgtgatgag
     6721 cagaatcacc accgccgtga tcgcgccgat cagcttagag ggcggtgtct ggccggcccg
     6781 cgcctccaga aaggtcagcc ccaaccgccc gggcagttcg tccgtaccga tcccgctgag
     6841 gaaggattcc accagcgccc ggaccacctt ggccacgaca tacccgatca acacgatgat
     6901 ggccgcgacc agcagggccg gcacggcatc gagaatcgtc tggagcatgg ccttgagcgg
     6961 atccgagatc gcacggatcc ccaggcagtc taccgcggag atgaggatcg ggatcaggat
     7021 gaagaacgaa accaccttgg cggctatggc ggagagacgg ttcccgccca gcgtccggct
     7081 cagaccgaac ttctccgccg cggcatccgc gccgctggcg gcgaggaagt tggtgaccac
     7141 ctcgcgcacg atcgcggtca cgacgcgtcc caccaccaga atcagggctg cggcaaagag
     7201 attgggcagg ataccgaggg ctttggagag catgtcctgc aacggcgcca ccaaggcctg
     7261 ctggtcgagc gcctgcaaaa acggcggaat gccgaagagc aggatgacgt agaagagcaa
     7321 ccggccgatc atcgcgccgg ggccaagcgg ttgactttcg tccgtagtcg gcagagacag
     7381 gtgtttgcca gcccgctcgt cgaacttcag tgcccccaga gcgcgtgtga ccccggcccg
     7441 taccacagcg gccagaatcc agtagaccag caggatggcg accgccttga tcaggttggg
     7501 gatcacgccg atgatgcggt caagcaggtt ctgtaacggc cctgccacca tcgagaggtt
     7561 cagcgcgttg aaaaacgcga cgaacccgaa cagcaccaac agccacttca cgatgttgcc
     7621 cacaaggctg gcgagcgaac aggtctcgcc ggtcggccgt ttcagaagac tttcgagtcc
     7681 ccaatcgcgc acggcacgtt catcaatccg ggttagattc agcagccggg tgacgcaggc
     7741 acccgcgaac ttgccgacca gccagaaggc caatagcaaa atcacagcct tgagcacacc
     7801 gggcagggcg gcggcaaacc gctgcatcgc gggcaacagc acgtcctgcc atagcttgcg
     7861 gctttgatca accgcctgcc ggcccccgct acacagcgag tctcccagtt ccgacgtggc
     7921 ttcgcccacg gcttgaacac tcttctttcc ggtcgccaaa acgcccgacg cggtttccga
     7981 aaccggttgt gccggcgcgg aggggtctcg cgtttgggta cgggctggga gggcgctcag
     8041 ccccacgacc agaatcaatc ccattccgat catcacaggg gcaatatggt gtcgtttcat
     8101 aggagggctc ctttccgagt tacggtttgt gagggtcagg catccgttct taccggactc
     8161 ctccggtcgc atgccgtccg tcaccattag gttaatattt ttcagctgcg catgcaatca
     8221 aaatgcgtat cgtaatcggt tcaccgcgaa gcgcaaatca gggccttgtg gtttctgtgg
     8281 ctctttgagg aaaaatccgc tactgtacgc gacaaagagg cggaaaagaa aatggcgcgc
     8341 cgacccgttg ggcaagatgc gggccgcgcg gtttttgaag cggttttcaa caacacaggt
     8401 ggtcatgtcc tccccacgca taacgaaaga agcgcgcgcg agttggctgc ggctctatga
     8461 gacggttgat cggatcgatc ggttgtcgcc gtggccgtgg atggagcccg cgtcgtgttt
     8521 cggcgtggcc gtgccggata tggaagagcc ctgctttgtg ttgttcggcg agccgggacg
     8581 ggagatgcgc gccatccgga ttctgctggg gtggaaggcg ttttacgaga tgatgacccg
     8641 tctcgccgat cccgcgaagc agggcgtggc ctggctgctg gaaatccgca tgatcgaggt
     8701 gctttgcgcg ccagaaccat tgctgttcca acacgagagg gactttctgc gcgcgattcg
     8761 gcgcgaaccg gatgggaaag gcgagtggac aatcttccgc tccatcgtgc cgggctatca
     8821 cccgtggctt ccggatgcaa aggaacgcaa gttgctggag gatgtgctgt atcaggcgtt
     8881 cggcatggcc ctgcgggtcg aagccgatcc cggcatgttg cgcagtcgtt tcccgcgcga
     8941 gatcctcatc cggacatcgg acgggaaggg cgggtggcgg gatgtgtggt cacgggtcaa
     9001 agaactgtcg ggcgaggaga tcgaggtccg cattgaatcc cggcggttgc aggcgctggc
     9061 cgctaagccg acatgcccgg tcaccttgca ggtggatctg ctgttcaccc cgttttcgat
     9121 ccagcctggc cgcgagcgcc cgcagaccgc gtatgtgctg ctggccgtgg atgcgcagag
     9181 cgggctgatt cattgcggtg agttgtttca ggcgatcgga gggatcgctg ccatgtggtc
     9241 agagattccc gaccggctga tcgcgttgtt cgagcgcttg ggcggatgtc ccgatgcgat
     9301 cgaggtgcgg ggggaccgca tggcgaacct catgcgcccg ctcaccgagt ttctgccgtt
     9361 caaactggtg cggcgcgaac acctgaagat gctggatgag gcgcgcgtgg ggcttagcgc
     9421 gtatctgcgg aacggggagg aggaagcgta gcgcggtgct gcccgcgccg ccgcagcggc
     9481 aggtacagcg caccggccag cagcatcgag gccccgaaag cgggcaggaa cgaatcggcg
     9541 aggcgacccc acgcgcctac ggcggcgtaa acggccgaga ccgccgcgtt gcccagcagc
     9601 gtgatggcga aggcccgtcc gaaaggttga cgcgccaggc cgctgaacac gaccgaggcc
     9661 tcggccagaa ccggcacggg gcgcagcgcc agcagcagcc agatcccgta ccgtgcgtgg
     9721 aaggattgaa gcagccggac ctcgtctgct ccgatcagac gctgcgccgc gggtgccgcc
     9781 atccgcccca tcagcaggcc gaccagcgcg ctggcgctca tgccgatgaa cgaggcgcag
     9841 gttccggcaa cgaaacccag tgtcatgccg caagcggtgc tgaccagact ggagggcacg
     9901 ggtaccacga tatctgatgc cagcagcgcg gtcagcagca ggccggtttg cagcgggtgg
     9961 gcctgcgcat ggcggagcag cgcgtcagtc caggcctgaa ccgcatccgc gaacaggaaa
    10021 aacggcagga tcaccgccaa gagcacgagc gtcagcaacg acagggttcg gtaaggcaga
    10081 tttggcatca ccccgtaacc atactaaacc gacatgccgt gcgcaaccat cacgcaagga
    10141 ttttgggcac agccatgact ttcgtgtgtt ctgtgtgtcc cgtggtgaag cgatcccgat
    10201 cccgatagcg atcccgatag cgatagcgat cccgatcccg atagcgattt ggatgaaacc
//
LOCUS       DAOSLE010000094        10105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6473_length_10105_cov_7.823582, whole genome shotgun sequence.
ACCESSION   DAOSLE010000094 DAOSLE010000000
VERSION     DAOSLE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_10105_cov_7.823582"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..2122)
                     /gene="aspS"
                     /locus_tag="PK576_07345"
     CDS             complement(332..2122)
                     /gene="aspS"
                     /locus_tag="PK576_07345"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE61445.1"
                     /translation="MHLYRSHTCNELRTTDAGTTAKLSGWIFRIRDHGGILFIDLRDH
                     YGITQVVVHPNRPFFEPLSKAKLESVITVTGEVTLREPSTINPDIPTGEVELVANECL
                     IEAASEQTPLYLPGEEDGAEDLRLKYRFLDLRRQRIHNNILLRSRIISFLRKAMEAHG
                     FNEYQTPILTCSSPEGARDYLVPSRVHHGKFYALPQAPQLFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDMEMSFVTQDDVFAVVEDVLHKTFTAFSKKHVDATPWIR
                     IPYREAMLKYGTDKPDLRIPIEMFDCSEIFKTSGFNAFAAAVKSGAVVRAIPVKGIKD
                     KPRSFFDKLVEHAKTLGSKGLAYLVWDGDTVKGPIQKFLTPEELAALASIADAKDGDV
                     IFFVCDEADTAAKIGGDIRAKLGRDLELIDKNVFRFCWIVDFPMFEKDPETGATAFSH
                     NPFSMPQGGMDALLNKDPLDILAYQYDVVCNGIELSSGAIRNHLPEIMYKAFEIAGYG
                     PEVVEEKFSCLLNAFKFGAPPHGGIAPGVDRMVMLLAGEDNIREVIAFPMNQKAQDLM
                     MNAPSEVTEKQLRELHIKIRGREQPAPVTG"
     gene            complement(2200..3129)
                     /gene="era"
                     /locus_tag="PK576_07350"
     CDS             complement(2200..3129)
                     /gene="era"
                     /locus_tag="PK576_07350"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE61446.1"
                     /translation="MSPSAPHGSKRCAVVGIVGRTNSGKSTLLNRMVGEKVSIVSPVM
                     QTTRSTIRGILTEDRGQLVFLDTPGLHKSEGTLGTLMNRMARQASAGVDLLLVVFDGG
                     NRPHLEDDGWMRRVLFAEHPVVFVLNKSDRSPFYETAFKAQWETVQTEKQMRREVVWV
                     TTSAIHPAGAVDLVDRIFALATPSDHYLFPEDVVTDYPRKLTIADVIREKFLVRLYEE
                     VPHELGVKVENIDEQPHGWTVSVTIYVNRPSQKGLVIGPKGRTLKYVRQCAEPELSDL
                     FGVKVVLDLWVKVEKDWMKNFWLLRQMGYAGTH"
     gene            complement(3126..4496)
                     /locus_tag="PK576_07355"
     CDS             complement(3126..4496)
                     /locus_tag="PK576_07355"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE61447.1"
                     /translation="MTLIEKILSNPQAYSLDVETLGECKIESPIRNREFIDTEERILI
                     TENVKKLKYVTDRLGTPPSFERAGPHQKIFHDPTWSRVGILTAGGLCPGLNHVIKGLV
                     EILMFDYGIKTIYGIRYGYAGLIPRYGYQPIMLNTDTVDTIHEQGGTLLGSSRGQQDT
                     GELVDALARMNINLLFCIGGDGSLRCARDIAEECKRRKLAVSVIGIPKTIDNDLHFVG
                     RSFGFETAVAEATQIIQAAHTEAKGTFNGIGLVKLMGRDSGFIAAYATLANPVVNFCL
                     IPELDFELDGPHGLLAALTYRFERFKDHVVIVVAEGAGQRHITDQPERRDPSGNILKK
                     DIGEYLHHRIRNHFAKLRREASVKYFDPSYTIRSVPAKGTDAIRCYMLARNAVHAAMA
                     GRTNCVVGNQNDWYTLVPIRLATINRQKVNLNSDLWRAVLDATGQALYFNGDSANGML
                     NGEAVP"
     gene            4608..4683
                     /locus_tag="PK576_07360"
     tRNA            4608..4683
                     /locus_tag="PK576_07360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4641..4643,aa:Lys,seq:ttt)
     gene            complement(4764..5732)
                     /locus_tag="PK576_07365"
     CDS             complement(4764..5732)
                     /locus_tag="PK576_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE61448.1"
                     /translation="MKKVLCLGLSPVLQRTLRFDRLTPDEVNRARQVMESAAGKALNT
                     ARALVTLGTPAITAGFNGGASGARVTAMLKQCGVGDAMTTMPSPTRLCTTLLDDHAGT
                     VTELVEEAPAPGRTALRRFIADNLSRIPGAALLAISGTLPPFAKDDFYVAFVRAARAA
                     GVPVVIDSHRTALIQVLFEQPLLAKLNVAELEATLGEKLNTEARILRGMRDLLNLGAA
                     NVFVTQGSRAAYLLTPRAAWRFTPPAIRERVNPIGSGDCTTAGIVHALLRGKRLPDAV
                     RLGLACGSANAETLTPGTFDARRARALCRTVTCEPRMVRETVPQKN"
     gene            complement(5729..6367)
                     /locus_tag="PK576_07370"
     CDS             complement(5729..6367)
                     /locus_tag="PK576_07370"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOE61449.1"
                     /translation="MFTGLVQRMGVIRRLERSGAGWSLAITHDPWPDALVIGESVAVQ
                     GACLTVTALLPDGFTADLLDETLQRTALQRLGPGARVNLERALALGDRLGGHLVSGHV
                     DECGTLLSVEARGRDAVWRVRCSPELASHTVRKGSVTLDGVSLTVTNVGENWIEVNLI
                     PHTLRITTLGDRQLGDALNVEGDLLGKYVARLLGRDTRGGLTAQMLEENGFI"
     gene            complement(6371..7468)
                     /gene="ribD"
                     /locus_tag="PK576_07375"
     CDS             complement(6371..7468)
                     /gene="ribD"
                     /locus_tag="PK576_07375"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOE61450.1"
                     /translation="MRDDRYWMRRALTLARLSEGRTRPNPPVGAVVTRAGRMLGAGRH
                     RRAGCAHAEIEALDACRTSARGATLYVTLEPCCTHGRTPPCTDRILREGITRVVVGCR
                     DENGCHCGRGLSLLQKQGVEVVCGVCEEEARELALPFFTRVTTGLPFVTLKLALTLDG
                     RIADRIHRSQWITGEAARVEVQRMRRRADAVMVGAGTVCADNPSLLCRLRHPDGAKLL
                     RVVVDATGRTPPDAQVFSDAAATRTLVATTPDGAARRGAAWAQHGARVWTFAPDKAGR
                     VPIRRLLRKLAAEGCLHVLCEGGGRLAGSLHDADAVDEYSFFYAPAVLGDSDAIGAVM
                     GCGNLLAGMRRLRCMEVNRFGADIRIRARRA"
     gene            complement(7489..7980)
                     /gene="nusB"
                     /locus_tag="PK576_07380"
     CDS             complement(7489..7980)
                     /gene="nusB"
                     /locus_tag="PK576_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE61451.1"
                     /translation="MATRRQGREWALQMLFQADLNPGLDLNLTIPKFWTQQYTCKLEE
                     AADKDLTLPEKPDRSVEDRVAPPKIREFSERLVRGVLDHLPELDAKLTSYTKNWPLHR
                     MGSVERNVLRLAFYEMLYCADVPPAVVLNEAIDLAKYFSNAEAGRFVNGVLDRLNKEL
                     NRT"
     gene            complement(8017..8478)
                     /gene="ribH"
                     /locus_tag="PK576_07385"
     CDS             complement(8017..8478)
                     /gene="ribH"
                     /locus_tag="PK576_07385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008289254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOE61452.1"
                     /translation="MVEITGQLDAKDMRLALVVSRFNHFFTEQLVKGAVDCFLRHGGK
                     EDALTLVRVPGANELPLTAQRLASAGKHHVVVVLGAVIQGATPHADLINGTVARALSK
                     IALDTGVPVINGVVCAQNLEQAVERAGTKHGNKGWDAVLAAIETVNVLRAL"
     gene            complement(8565..9773)
                     /locus_tag="PK576_07390"
     CDS             complement(8565..9773)
                     /locus_tag="PK576_07390"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOE61453.1"
                     /translation="MEKDSVFDPVETCIEAFGRGEMVVVADDENRENEGDLIIAAEKI
                     TPEAINFMVVNARGLVCVPMTGARLRELGVERAPSRNRGDKFATAFTYSVDAATGVTT
                     GISAADRAWTVKTLLDPASGPESIVSPGHIFPLEAREGGVLVRAGHTEAAVDLAKLAG
                     LKPAGVICEIMLPDGSMARLPDLREFATRHGLKMLSIADLIQYRRHRERLIDLVEEVE
                     LPTAYGQFTLRLYVSSPDGREHLALIKGNLAKADAPLVRVHSECLTGDVLGSLRCDCG
                     NQLHAAMRRIDQEGCGVVLYMRQEGRGIGLANKLHAYKLQQNGFDTVEANIELGFPAD
                     LRDYGVGAQILADIGLKRIRLMTNNPKKLIGLQGYGLEIVERVPVVFEPGTHNRRYLE
                     TKKNKMGHLI"
     regulatory      complement(9835..9979)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1829 a         3082 c         3238 g         1956 t
ORIGIN      
        1 cgcagggctg acgtattgag atgcgggcga agcccgcaac ctcaaaggcg gtgaggctgt
       61 gaggctgtga ggctgtgagg ctgtgaggct gtgagactgt gaggctgtga gactgtgagg
      121 cggtgagact gtgaggcggt gagactgtga ggcggtggtg aaacagtcga tcccgatagc
      181 gatcccgata gcgatcccga ccccgatagc gatcccgata ccgatagcga tcccgacccc
      241 gatgaaacct tgaaacccag aaaccttgaa acccagaaac cctgaaaccc cgaaaccaaa
      301 gagagttgaa ggttcaactg ctctttttag gttaaccggt gacaggcgcg ggttgctcgc
      361 gtccgcggat tttgatgtgg agttcgcgca gttgtttttc ggtgacttcg gagggcgcgt
      421 tcatcatcag gtcctgcgcc ttctggttca tggggaaggc gatgacttca cggatgttgt
      481 cctcaccggc cagtagcata accatgcggt cgacgccggg ggcgatgccg ccgtggggcg
      541 gtgcgccgaa tttgaaggcg ttgaggaggc aggagaactt ctcttccaca acctcggggc
      601 cgtaaccggc aatctcgaag gctttataca tgatctctgg gaggtggtta cggatcgcgc
      661 cgctggaaag ctcgatgccg ttgcagacca cgtcgtactg gtaggcgagg atgtcgagcg
      721 ggtccttgtt gagcagcgcg tccatgccgc cctggggcat ggagaagggg ttgtgcgaaa
      781 aagcggtcgc gccggtctcg gggtctttct cgaacatcgg gaagtcaacg atccagcaga
      841 agcggaagac attcttgtcg atgagttcga ggtcgcgtcc cagtttggcg cggatgtcgc
      901 cgccgatctt ggcggccgta tcggcttcgt cgcagacgaa aaagatgacg tcgccgtctt
      961 tcgcgtccgc tatggaggcg agagccgcaa gctcttcggg cgtgaggaat ttctggatcg
     1021 ggcctttgac ggtgtcgccg tcccagacga ggtaggcgag gcctttggag ccgagggtct
     1081 tggcgtgctc gacgagctta tcgaagaagc tgcggggctt gtccttgatg cctttgaccg
     1141 ggatcgcgcg gacgacggca ccggatttca cggcagcggc gaaagcgttg aagccggagg
     1201 tcttgaagat ctcggaacag tcgaacatct cgatggggat gcgcaggtcg ggcttgtcgg
     1261 taccgtattt gagcatcgct tcgcggtaag ggatgcggat ccacggcgtg gcatcgacgt
     1321 gctttttgga gaaggcggtg aaggtcttgt ggagcacgtc ttcgaccacg gcgaagacgt
     1381 catcctgcgt gacgaacgac atctccatgt cgagctggta aaactcgccg ggagagcggt
     1441 cggcgcgggc gtcctcgtcg cggaaacagg gtgcgatctg gaaatagcgg tcgaaccccg
     1501 cgaccatcag gagctgcttg aagagctgcg gagcctgcgg cagggcgtag aacttaccat
     1561 gatgcacgcg gctgggcacg aggtagtcgc gcgcgccttc gggcgagctg cacgtcagga
     1621 tgggggtctg gtactcgttg aacccgtgcg cctccatcgc cttgcgtaga aaagagatga
     1681 tgcgggagcg caggaggatg ttgttgtgga tgcgctggcg gcgcaaatcg aggaagcggt
     1741 acttgaggcg cagatcctcg gcgccgtcct cctcgccggg caggtagagc ggcgtctgct
     1801 ctgaggcggc ctcgatgagg cactcgttgg cgacaagctc gacctcgccg gtggggatat
     1861 ccgggttgat ggtcgaaggc tcgcgcagcg tcacttctcc ggtcacggtg atcacgctct
     1921 ccagcttggc tttggacagc ggttcgaaga acgggcggtt cgggtggaca accacctggg
     1981 tgatgccgta gtggtcgcgc agatcgatga acaggatgcc gccgtgatcc cggatccgga
     2041 agatccagcc ggaaagcttg gcggtcgtgc ccgcgtcggt ggtgcggagt tcgttgcagg
     2101 tgtgtgaacg gtacaggtgc atggtttcgg tttccttaaa aagtggtaaa acgataacaa
     2161 aacggcgcac gggatgccac gagggatttt tgcgcgggct cagtgagtgc ccgcgtatcc
     2221 catctgccgc aggagccaga agttcttcat ccagtccttc tcgactttca cccacaaatc
     2281 gagaacgacc ttgacgccga agagatcaga caactcgggt tcggcgcact gccgcacata
     2341 tttgagtgtc cggcctttgg gtccgatcac gagacccttc tgcgaggggc ggttgacata
     2401 gatcgtgacc gacaccgtcc agccgtgcgg ttgctcgtcg atgttttcca ctttgactcc
     2461 cagttcatgc ggcacctcct cgtacagccg gaccagaaat ttttcgcgga tgacatcggc
     2521 gatcgtcagc ttgcggggat aatccgtgac cacgtcctcg ggaaacaggt agtggtcgga
     2581 gggcgtggcc agggcgaaga tccggtcaac cagatcgacg gcgccggccg ggtggatcgc
     2641 agaggtggtg acccagacga cttcacgccg catttgcttt tcagtctgaa ccgtttccca
     2701 ctgcgctttg aaagccgttt cgtaaaaagg tgaacggtcg cttttgttga ggacgaaaac
     2761 gaccgggtgc tcggcgaaca gcacgcgccg catccaaccg tcatcctcca gatgcgggcg
     2821 gttgccgcca tcgaacacga ccagcagcag atcgaccccg gcggaggcct ggcgggccat
     2881 gcggttcatg agcgtcccca gcgtgccttc cgatttatgc aggccgggcg tgtcgagaaa
     2941 cacgagttgg ccgcggtctt cagtcaagat gccgcggatc gtggagcgcg tggtctgcat
     3001 caccgggctc acgatgctca ccttttcgcc gaccatgcgg ttgagcagtg tggacttgcc
     3061 ggagttggtg cgcccgacga tgccgaccac ggcgcagcgc ttggatccgt gcggggcgga
     3121 aggactcatg gcaccgcttc cccgttcagc atgccgttgg cggagtcgcc gttgaaatag
     3181 agcgcctggc ctgtggcgtc caacacggcg cgccagagat cgctgttgag gttcaccttc
     3241 tgcctgttga tggtcgccag acggatcggc accagcgtgt accagtcgtt ttggttcccc
     3301 accacgcagt tggtgcgtcc ggccatggcg gcatgcaccg cattgcgggc cagcatgtaa
     3361 cagcggatcg cgtccgtgcc tttggcgggc acgctccgga tggtgtaact ggggtcgaag
     3421 tatttgaccg acgcctcgcg gcggagtttt gcgaagtgat tccggatgcg gtgatggaga
     3481 tattcgccga tgtccttttt gaggatattg cccgaggggt cgcgtcgctc gggttgatcc
     3541 gtgatatggc gctgtcccgc gccttccgcc accacgatca ccacatgatc cttgaagcgc
     3601 tcgaaacggt aggtcagcgc ggccagcaga ccatgcggcc cgtccagttc aaaatcgagt
     3661 tcgggaatca ggcagaagtt caccaccggg ttggccagcg tggcgtaagc cgcgatgaac
     3721 cccgaatcgc ggcccatgag tttcacgagc ccgatgccgt tgaacgtgcc tttggcctcg
     3781 gtgtgcgctg cctgaatgat ctgcgtggcc tctgccaccg ccgtctcgaa tccgaaactg
     3841 cgtcccacga agtgaaggtc gttgtcgatg gtcttgggga tgccgatcac gctaacggcc
     3901 agtttccgac gtttacactc ctcggcgatg tcgcgcgcgc aacgcagtga tccgtcgccg
     3961 ccgatgcaga agagcaggtt gatgttcatg cgggccaagg cgtcgaccag ctcgccggta
     4021 tcctgctggc cacgggaaga gcccagcaag gtgccgccct gctcatggat cgtgtccacg
     4081 gtgtcggtgt tgagcatgat cggctgatag ccgtaacgcg ggatcaatcc ggcatagccg
     4141 tagcggatgc cgtagatcgt cttgatcccg tagtcgaaca tcaggatctc caccaggccc
     4201 ttgatcacgt ggttcaggcc cgggcagagc ccgccggccg tcaggatgcc gacgcggctc
     4261 caggtggggt cgtggaaaat cttctgatgc ggtccggcgc gttcaaagga ggggggggtg
     4321 ccgaggcggt ccgtgacgta cttcagcttc ttgacgttct ccgtgatcag gatgcgctcc
     4381 tccgtgtcga tgaactcgcg gttgcggatc ggtgattcga ttttacattc accgagcgtc
     4441 tcgacgtcga gcgagtacgc ttgcggattg gagaggatct tttcgatgag cgtcatgacg
     4501 gttgttccct gttacggcgt gaagcgtacc attatacgtg attcgtgggg atggtcaatt
     4561 gacacccgta cggatttctg atttaatccc actcttcctg atgccattgg accatagctc
     4621 agtcggtaga gcacgacact tttaatgttg gggtcgtggg ttcgaatccc actggtccaa
     4681 ccatttcgca tggatgaaaa agacgtccgt ccctgggcga aaacggggag atacccggaa
     4741 cgctgaacat cgacctgcga cggtcagttt ttttggggga cggtttcccg aaccatccgc
     4801 ggctcgcacg tcacggtccg gcacagggcg cgcgctcggc gggcgtcaaa cgtgccgggc
     4861 gtcagcgttt cggcattggc gctgccgcag gccagaccaa gccgcacggc atccggcagg
     4921 cgtttgccac gcagtagcgc atgcacaatg ccggcggtcg tgcagtcgcc gctgccgatg
     4981 gggttcacgc gttcgcggat cgcgggcgga gtgaagcgcc acgcggcgcg gggcgtcagc
     5041 agatatgcgg cgcggctgcc ttgggtcacg aagacgttgg cggcacccag attcagtaga
     5101 tcccgcatgc cgcgcaggat gcgcgcctcc gtgttcagtt tttcgccgag tgtggcctcc
     5161 agttccgcaa cgttcagttt ggccagcagc ggttgttcga acagtacctg gatcagcgct
     5221 gtacggtgcg aatcgatcac caccgggact ccggcggcac gcgcggcgcg tacgaacgcc
     5281 acgtaaaaat catcctttgc gaaaggcggc agcgtgccgc tgatggccag cagggcggca
     5341 ccgggaatcc ggctcaaatt gtccgcgatg aaccggcgca aggcggtgcg gccgggtgcg
     5401 ggcgcctcct ccaccagttc cgtcacggtc ccggcgtggt cgtcgagcag ggtggtacag
     5461 agacgcgtgg gggagggcat cgtcgtcatc gcatcgccga ctccgcactg tttcagcatg
     5521 gcggtgaccc gtgcaccgct cgcgccgccg ttgaatcccg ccgtgattgc aggggtgccc
     5581 agagtgacga gggctcgtgc ggtgttgagc gccttgcccg ccgcgctttc catgacctgg
     5641 cgtgcgcggt tgacctcatc cggcgtaagc cggtcaaacc gtagggtacg ttgcagtacc
     5701 ggggacaatc cgagacagag gacttttttc atatgaatcc gttttcttcc agcatctgcg
     5761 cggtgaggcc accgcgggta tcgcgcccca gcaggcgcgc cacatatttc cccagcaagt
     5821 cgccctcgac gttcagcgca tcgccgagtt ggcggtcgcc gagcgtggtg atacggagcg
     5881 tgtgggggat caggttcacc tcgatccagt tctcgcccac atttgtgacc gtgaggctga
     5941 cgccgtccag cgtgacggag cctttcctga ccgtgtggct ggcgagttcg ggcgagcagc
     6001 gcacacgcca gaccgcatcg cgtccgcgcg cttcaacgga aagcagcgtg ccgcactcgt
     6061 cgacatgtcc gctcaccaga tgaccgccca gacgatcgcc cagcgccagc gcgcgttcga
     6121 ggttgacgcg cgcgcccgga ccgagccgct gcagggcggt gcgctgcagg gtctcgtcga
     6181 gcaggtcagc ggtgaacccg tccggaagga gggcggtcac ggtcaagcag gctccctgta
     6241 cggccacgct ctcgccgatc accagagcat ccggccacgg gtcatgggtg atggcgaggg
     6301 accagcccgc gccagagcgt tccagacgcc ggatcacacc catccgttgc accaatcccg
     6361 taaacatggg ttaagccctc cgtgcgcgga tccggatatc cgcaccgaac cggttcacct
     6421 ccatgcaacg caaccgccgc atgccggcca gcaggttgcc gcatcccatg acggcgccga
     6481 tcgcgtcgct gtcgcccagc accgccggcg cgtaaaaaaa ggagtactca tccacggcgt
     6541 ccgcatcgtg cagtgaccct gcgaggcggc cacccccttc gcacagcaca tggaggcacc
     6601 cctccgcagc gagtttccgc agcaggcgcc ggattggcac acgcccggct ttgtccgggg
     6661 cgaacgtcca aacccgcgcg ccgtgctgtg cccacgccgc gccgcgccgc gccgcgccgt
     6721 caggtgtggt ggccacaagg gtgcgtgttg ccgcggcatc cgaaaacacc tgcgcgtcgg
     6781 ggggcgtgcg gcccgtggcg tccaccacca cgcgcagtaa ttttgcgccg tcgggatgcc
     6841 ggagacggca gagcagcgag gggttgtccg cgcaaaccgt accggcgccg accatcaccg
     6901 catcggcgcg tcggcgcatg cgttgcactt cgacccgcgc cgcctcgccc gtgatccatt
     6961 gcgagcggtg gatacggtcg gcgatgcgcc cgtccagagt caaggccaat ttcagcgtga
     7021 caaagggcag gccggtggtg acccgcgtga aaaaaggcag ggccagttcg cgggcctcct
     7081 cttcgcagac tccgcagacg acctccacgc cttgcttttg cagcagcgag aggccgcgtc
     7141 cgcagtggca gccgttctca tcccggcaac ccaccaccac gcgcgtgata ccctcgcgca
     7201 gaatgcggtc cgtgcagggc ggggtgcggc cgtgcgtgca gcacggttca agcgtcacgt
     7261 acagtgtggc accgcgggca gatgtccggc aggcgtccaa cgcctcgatc tcggcatggg
     7321 cgcatccggc gcggcggtgt cggcctgcgc ccagcatccg gcccgcgcgc gtgaccaccg
     7381 cgccgacagg cgggttgggg cgcgtccgac cttcgctcag gcgcgccagc gtcagggccc
     7441 ggcgcatcca atatctgtcg tcccgcatct cccgcgtcct cccgtgtgtc acgtccggtt
     7501 gagttccttg tttaaccggt cgagcacgcc gtttacgaag cgtccggctt cggcgttgct
     7561 gaagtacttg gcgaggtcga tcgcctcgtt gagcaccacg gccggcggca cgtcggcaca
     7621 gtacagcatc tcgtagaagg cgaggcgtaa cacattgcgc tcgaccgaac ccatgcggtg
     7681 cagcggccag ttcttggtgt atgaggtgag tttcgcgtcc agttcgggaa gatgatccaa
     7741 gaccccgcgc acgagccgct cgctgaactc ccggattttc ggcggggcga cgcggtcttc
     7801 caccgaccgg tcgggctttt ccgggagcgt caaatcctta tcagcggcct cctccagctt
     7861 gcaggtatac tgctgggtcc agaatttcgg gatggtcagg ttgaggtcca atcccggatt
     7921 caggtccgcc tggaaaagca tctgcagcgc ccattcccgc ccttgtctgc gtgtcgccat
     7981 ggagactcct tgcctcgcgt tccggttttc agacgcttac agcgcccgga gcacattcac
     8041 cgtctcgatc gcggccagaa ccgcgtccca tcccttgttg ccgtgcttcg tgccggcgcg
     8101 ttcgaccgcc tgttcgagat tctgcgcgca gaccacgccg ttgatgaccg gcacaccggt
     8161 atcgagcgcg attttcgaca aggcccgcgc caccgttccg ttgatcaggt cggcgtgcgg
     8221 ggtcgcgcct tggatgacgg cacccagtac cacgacgaca tggtgtttgc cagccgatgc
     8281 cagtcgttgc gcggtgagcg gcaactcatt cgctccgggc acccgcacga gcgtcagcgc
     8341 atcttccttg ccgccgtgac ggaggaagca gtcaacagcg cccttcacca gctgttcggt
     8401 gaaaaaatga ttgaaccggc tcaccaccag cgcgagccgc atgtcctttg catccaattg
     8461 tcccgtgatt tccaccatgg cgttctcctg taacgcgttc cgtgttttcg tttcccgcgc
     8521 cgtttccggc acgccgcatc ggacgccctg tccgggttcc gaaatcagat caggtgaccc
     8581 attttgtttt ttttggtttc gaggtagcgc cggttgtgcg tgccgggctc gaagacgacc
     8641 ggcacacgct ccacgatctc cagcccgtac ccttgcaggc cgatcaactt tttggggttg
     8701 ttggtcatga gccggatacg tttgagtccg atatcggcca gaatttgcgc gcccacgccg
     8761 tagtcgcgca ggtcggcggg gaagcccaac tcgatgttgg cctccacggt gtcgaacccg
     8821 ttttgctgca atttgtaagc atgcagtttg ttcgcgaggc cgatgccgcg gccctcctga
     8881 cgcatgtaca ggaccacgcc gcacccctcc tgatcgatcc tgcgcatggc cgcgtggagc
     8941 tggttgccgc agtcgcagcg caacgagccg agcacatcgc cggtcaggca ttcgctatgc
     9001 acgcgcacca agggggcatc ggctttggcg agattcccct tgatcaacgc gagatgctcc
     9061 cggccatccg gcgacgaaac atagaggcgc agcgtaaact gaccgtatgc ggtcggcaac
     9121 tcaacctcct caacgagatc aatcagccgt tcccggtggc gacggtactg gatcaggtcc
     9181 gcgatcgaca gcatcttgag cccgtgccgc gtggcgaatt cgcgtaaatc cggtaaacgc
     9241 gccatcgagc cgtccggcaa catgatctca cagatcacgc ccgccggctt gagcccggcc
     9301 agtttggcga gatcgacggc cgcctcggta tgaccggcgc gcaccagtac cccgccttcg
     9361 cgcgcctcca gcggaaagat gtggccgggg ctcacgattg actcaggacc ggaggcggga
     9421 tcgagtagcg tcttgaccgt ccacgcgcgg tctgcggcac tgatgccggt cgtcacgccg
     9481 gtggcggcgt caacggaata agtgaaggcc gtcgcaaatt tatctccccg gttgcggctc
     9541 ggtgcgcgct ccacgcccaa ctcgcgcagg cgggcaccgg tcatcggcac gcagaccagc
     9601 ccgcgcgcgt tgacaaccat gaagttgatg gcttccggcg taatcttctc cgccgcaatg
     9661 atcaggtcgc cctcgttctc gcggttttcg tcatccgcga caaccaccat ttctccccgc
     9721 ccgaaagctt cgatacaggt ttcgacagga tcgaacaccg aatccttttc catcgccgcc
     9781 acctcaaaaa aaacccggtc cacaaacggg accggggcaa aagcaacaca ggcttcacat
     9841 cttctttcat ccagactaga ctgtcggcca cggaatcgaa ccgtgtcagt ggcgcgcagg
     9901 aacagcgtgc cagtcgcggg ctataccgcc ggtcaggact ctcccgtaaa cacgggatca
     9961 ccttgccccg aagacgtttg aaacggcatc actgtaatcc tttggaccgg atttggcaat
    10021 accggaagtc aaaaagaacg ggcgaacgtt caacccaaaa ggagcagttg aacgggtaga
    10081 attccagcaa agccccgtaa acatt
//
LOCUS       DAOSLE010000095         9954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6571_length_9954_cov_6.439135, whole genome shotgun sequence.
ACCESSION   DAOSLE010000095 DAOSLE010000000
VERSION     DAOSLE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_9954_cov_6.439135"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PK576_07395"
     CDS             <1..873
                     /locus_tag="PK576_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61454.1"
                     /translation="AMFMVFGGTGPLVGNNGIYQNYGEGGLYRGDNAAALGPAAPLIY
                     PNGWYANSYSLAKGYCTYVKVNGVETDPTTTGLDNDLNVISLRTYIDPVSASAFNCIH
                     YNNKFGGAQYGEVMFYTRPVSRPEAHRIETYLRNKWCREGYSTAGYEAAVAGKVMLGT
                     NAMLKVVGGEPLTATGLGGGGTVTGDVTLQNNAELTVVITDGVASSQTIEGELVLPVN
                     GTIKVVGDFTGLAFGDSDVFKSASIHASTDLSGWGRNLTQVVHPEKRNVSFRVVEGAI
                     RMTVSKSGTTFILR"
     gene            1034..3133
                     /locus_tag="PK576_07400"
     CDS             1034..3133
                     /locus_tag="PK576_07400"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOE61455.1"
                     /translation="MTKMNDTPIATAVAVLLLAIACASKVAADELQDCELKRLPLGSI
                     CPEGWLKHQLDKMTDGICGRLYEYGKFLNPTNGWISRAEQGELDNQRHWNEGTGWEEQ
                     PYWFRSFVKLAALTGNERMLGVCRDWTEKMLATRDPDGWYGPQPLKRVRLKDGTFMQD
                     IWGHMVMNEALLSWYDYSGDRRVMALLSGFCDFCLAVPEDRFIHPPQGNGYAPASGWL
                     PLIQKARAGDMISTLFRLYDVTKDAKFTRLSDKVWRRYYVKRESLFLNIHNVNFCQMF
                     SYPSIYARRTGDLALAEESEYWLKQHWMTWGQMPRCAFAADENCRVGCIDPRYGLESC
                     SWGEYVRSFQNLSDHRAETKWADRTEDVVFNHAPCAYTPDWNEVHYITACNQTVIDAR
                     TDHDYANHPPMNAYSHTEYRCCLYNAAMPFPLFTENLVKRTRDGALVFWMYAPCSGKT
                     DGVEWKLDTRYPFRETARLAVNAAKPLVLRFRVPAWAKAFSVGGTAATPGVRMLDVKV
                     PAGGSTLDIAMKAECEFTPWARQNGISVDRGPLTYSVAIGERYSKVSVVKGDSGSVIW
                     VEGNNNRDADRVYTEIAPTTPWNYGIDVTAKPVWKECVWNDDCFVSSNACCELSVRCR
                     RIPEWTLQDNEPARLQLSPAYSSEPLETVRFTPLCCQRLRLSVLPQVTDNATLGYRWK
                     KVPPTTDRKNRAKIFSP"
     gene            complement(3260..4555)
                     /locus_tag="PK576_07405"
     CDS             complement(3260..4555)
                     /locus_tag="PK576_07405"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOE61456.1"
                     /translation="MTTTMKTALASLAACLALNSLAADISHVSVRQRWPWSKLVDIDY
                     VIANATQGVDIAVTAHNGSIPLVLGDNELSGDLYGVSGQGARRIVWDPTQSQYTNEEV
                     LSQFKVTLTPVTPPLYMIVDLTKEAGADKQIEYVYEEDLTNGLWGAWMRNPITNAVGE
                     AIVESVIWTGVTTNDLYKTDKLVLRRVPAGSFQQGDTQNGADNVTLTKNMYAGVFEVT
                     QKQWDHVMGTTGGTATQAKHTVSYYEIREDPANADDPAVNWPSNRIVNATSFMGRLRA
                     KTGIGDFDLPTEAQWEYVCRAKTTTVFNDGNTKAKYTGTEDNNNGNTNEHLNALGWYK
                     FSAPTPPTAAQLVGSKRPNAWGLYDMHGNVWEWCLNWGTTSAETGTDPDGADSGSKRV
                     FRGGSVYDSASYCRSAFRYQDPPGSRFFSLGFRLFRTLP"
     gene            complement(4666..5367)
                     /locus_tag="PK576_07410"
     CDS             complement(4666..5367)
                     /locus_tag="PK576_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61457.1"
                     /translation="MSVGNIAITVAVALLLVGQSAQAASSVPVMINTSPARDGHWSRV
                     CTNTVLLSWEWRDESAVRAELTLDGLRGTQTVVFTRPATNWVWQVSATPVPSEEDVVD
                     LTLRFYDAQNAVIRVQQSRLSVLSGAYGETPVLLSDADKRWTHVRDAAVIPHDVRWIN
                     TTAPASNSRLSIRRQDGVAQTNLLPAAGYYGWKLRDGSWGYGIFDLALDFPGSAGLWE
                     ATLLRVPEGSIFSVK"
     gene            complement(5794..6990)
                     /locus_tag="PK576_07415"
     CDS             complement(5794..6990)
                     /locus_tag="PK576_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOE61458.1"
                     /translation="MAKVLIIGAGGVGAVVAHKCAQVPEVFTDIVIASRTRARVDALA
                     ARLPRKVTTAQVDADNVPQTVRLLKRHRPDLVINVALPYQDLHIMDACLEVGVNYLDT
                     ANYEPPDQAKFEYKWQWAYHSRFKKAGLMALLGSGFDPGVTSVFCTYARKHFVPHVEE
                     IDIIDCNAGSHGQPFATNFNPEINIREVTAKGRFWEDGEWKETEPLSVCSSFTMPENL
                     GSLKVYLMYHEELESLARHIPELRRARFWMSFSDNYLKHLEVLRNVGMTRIDPVRFNG
                     VDIVPLQFLKALLPDPASLGPLTKGKTCIGCQVRGRNARGQRKTVFIYNICDHEECYR
                     EVQSQAISYTTGVPAMIGAKMMLTGQWRGKGVFNMEQFDPDPFMADLTVYGLPWTVLK
                     KAVFKK"
     gene            7155..8057
                     /locus_tag="PK576_07420"
     CDS             7155..8057
                     /locus_tag="PK576_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOE61459.1"
                     /translation="MKVLFLFIDGVGLRPPATDNPVHSGICPTLCHLIEQHGVSIDAC
                     LEVTGLPQSATGQTALFTGVNASQIMGRHCEGFPGPTLRKLIEQGNLFMDLSRKGLRC
                     RFADAYLVDSVDELRGRRFKSVTTVMALTQPETISIQDDLMANQAVFHDITRKSLQEK
                     GLAIPLVTPPQAAEHLIEVALANDFTLFEFFQTDLAGHSCQYDQACETLRTLDLFLDM
                     AVRLCKTTGLLLILTSDHGNIEDMGTRGHTRNPVPLIALGPGADEIKNLASSLTDITP
                     RLTRLMVPGHTSMPRTAPPADSAQ"
     gene            complement(8157..9353)
                     /locus_tag="PK576_07425"
     CDS             complement(8157..9353)
                     /locus_tag="PK576_07425"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE61460.1"
                     /translation="MGKQKTQVEHEIALRVGARIRQLREIQRISQIKLATEIGIRAGP
                     LGWIEKGKHLPSGRVLYRLAKQLNVRIDDLFQEQNVWEESAAARDGKQPVMLPPMDNG
                     INAGAEPLKSAHIICLSVAQALLKLETLCGVVKTSSLALTIPFAPTERGAEYLAGRLR
                     QDLGIGETVTHGYLEALESAGVRVIFLDMPEGCPTFSGYDPQNRNALIFVNSRLKKQP
                     ELQLFRLAFELGRVCWYVRETGEGQGSGGPLPENGAETLSEAEFARRFAAFFLMPARA
                     VQASVWQLGLSPVGWTWELLLRLKKRYGVSAQRFALRLQALGLSWTDKQKKTPSAYRF
                     KEEIEAFYDANGKSAEPGGTRPPLVMNGRLGDLLLCAERLKEKETAKAVHAVKRVLRQ
                     AGVRID"
     gene            complement(9573..>9954)
                     /locus_tag="PK576_07430"
     CDS             complement(9573..>9954)
                     /locus_tag="PK576_07430"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOE61461.1"
                     /translation="VMESPSVLLKGMEGSRLPVAAAHGEGLAVFASPEEEAQAIAALR
                     YVDGCGQPTERYPWNPSGTKGGLTGFTSSDGRATILMPHPERGFRSVQLSYRPADLFT
                     GEAGPWMRLFRNAYAFVTGTAVRA"
BASE COUNT         1980 a         3148 c         2848 g         1978 t
ORIGIN      
        1 gcgatgttca tggtgttcgg cggcaccggt ccccttgtag ggaataatgg catttatcaa
       61 aattatgggg agggcggcct ctaccgcggt gacaacgcgg ccgcactggg accggcggcg
      121 ccgctgatct atcccaatgg ctggtatgcg aattcctatt cgctcgccaa ggggtactgt
      181 acatacgtga aggtaaacgg tgttgaaacc gatccgacga caacggggct cgacaacgat
      241 ctcaatgtca tctcgctcag gacctacatc gatccggtga gcgcctcggc tttcaactgt
      301 atccactaca acaacaagtt cggtggcgcg cagtatggcg aggtgatgtt ctacacacgg
      361 ccggtgtcgc gtcccgaggc gcaccgcatc gaaacgtacc ttcgtaataa gtggtgccgg
      421 gagggttatt caacggccgg ttacgaggcg gccgtggcgg gcaaggtgat gctggggacg
      481 aacgcgatgc tgaaagtggt gggcggcgag ccgctcacgg caacgggtct cggcggcggt
      541 ggcacggtca ctggtgacgt gacactgcag aacaatgcgg agctgacggt ggtgatcacc
      601 gacggggtgg cgtcctctca gacgatcgaa ggtgaactcg tcctcccggt gaacggcacg
      661 atcaaggtcg tcggcgattt caccggcctc gcctttggag attccgatgt cttcaagtct
      721 gcctcgatcc atgcgtcgac cgatctgagc ggatggggcc gcaatctcac ccaggttgtc
      781 catcccgaaa agcgcaacgt ctccttcagg gtggtcgaag gcgcgatccg catgacggtc
      841 agcaagtcgg gaacgacatt catcctccgg tgatcgtgaa aacgataccg cagattcggc
      901 acctgtaagc aaaggggtgc gggctccgtc cgcattactt cgccggggtt tttgagaacg
      961 aggggcggtg gcgacgtttt cagcgcaacc caatgtgcaa acacgcctcc ttcgccccca
     1021 aggagaaacg cacatgacaa agatgaacga tacgcccatt gcgaccgccg tggctgtgtt
     1081 gctactggcg atagcgtgtg cctcgaaggt cgcggcggac gaattgcagg actgtgagct
     1141 gaagcgactg ccgctcgggt caatctgccc ggagggatgg ctcaaacacc agctcgacaa
     1201 aatgacggac ggcatctgtg ggcgacttta cgaatacggc aagttcctga accccaccaa
     1261 cggctggatc tcgcgcgccg agcagggcga actcgacaac cagcgccact ggaacgaggg
     1321 caccggatgg gaggaacagc cctattggtt ccgttcgttc gtgaagctcg ccgcgctgac
     1381 cggtaacgaa cggatgttgg gcgtctgccg cgactggacg gagaagatgc tcgcgacgcg
     1441 cgatccggac ggctggtacg gtccccagcc gctcaagcgg gtacggctta aggacgggac
     1501 tttcatgcag gacatctggg gccacatggt gatgaacgag gcgctccttt cgtggtatga
     1561 ctactccggc gaccggcgcg tgatggccct gctttccggc ttctgcgatt tctgtcttgc
     1621 cgttcccgag gaccgcttca tccacccgcc gcagggcaac ggttacgcgc cggcctcggg
     1681 atggctcccc ctcatccaga aagcgcgcgc cggcgatatg atctcaacgc tgttccggct
     1741 ttatgacgtg acgaaggatg cgaagttcac gcggctttcg gacaaggtgt ggcggcgtta
     1801 ctacgttaag cgcgaatcgc tcttcctcaa catccacaac gttaatttct gccagatgtt
     1861 ctcctatcct tcgatctacg cgcggcgcac cggcgatctc gcgcttgccg aggagagcga
     1921 gtactggctg aagcaacact ggatgacctg gggacagatg ccgcgttgcg cgttcgcggc
     1981 ggatgagaac tgccgcgtcg gctgtatcga tccgcgttac ggactggaga gctgctcgtg
     2041 gggtgaatac gtgcggagct tccagaacct ctccgaccac cgcgccgaga cgaagtgggc
     2101 agaccgcacc gaggatgtgg tcttcaacca cgctccatgt gcctacacgc cggattggaa
     2161 cgaggtccac tacatcaccg cctgcaacca gacggtgatt gatgcgcgca ccgaccacga
     2221 ctatgcgaac catccgccga tgaatgccta ttcgcacact gagtaccgct gttgcctcta
     2281 caacgcggca atgccgttcc cgctcttcac cgagaatctg gtgaagcgca cgagggacgg
     2341 cgcactcgtc ttctggatgt acgcgccgtg ctcggggaaa acggacggtg tcgaatggaa
     2401 actcgacaca cgctatccgt tccgcgaaac cgcccggctg gcggtgaacg cggcgaaacc
     2461 gcttgtcctg cgcttccgcg tacccgcttg ggcaaaggcg ttctccgtcg gcgggaccgc
     2521 cgcgacaccc ggcgtgagaa tgctggacgt gaaggtgccc gccggcggat ctacgctcga
     2581 catcgcgatg aaggcggagt gcgagttcac accgtgggca cgacagaacg gcatttcggt
     2641 cgaccgcggc ccgctcacct attcggtggc gatcggcgag cggtattcca aggtgtcggt
     2701 ggtgaagggc gatagcggtt ccgtcatctg ggtggaggga aacaacaacc gtgatgccga
     2761 ccgcgtctac accgagatcg cgccgaccac gccttggaac tacggcatcg atgttaccgc
     2821 gaagccggtc tggaaggagt gcgtgtggaa cgacgactgc ttcgtctcgt ccaacgcctg
     2881 ctgcgagctg tcggtccgct gccgacggat ccccgagtgg acgctgcaag acaacgaacc
     2941 ggcacggctc cagttgtcgc ccgcgtattc gagcgagccg ctcgaaacgg tgcgcttcac
     3001 gccgctctgc tgccagcggc tccggctctc ggtgctgccg caggttacgg acaacgcgac
     3061 gctcggctac cgctggaaaa aggttccgcc gacgacggac cgcaaaaacc gcgcgaaaat
     3121 cttcagcccg taatgaaacc gtgcggcagg tcgagggctc ttccactcga cccggttggc
     3181 aaagctcatc atcgtgttca ggacagcacg gggcgcggtt tttcgcccgc tcccgctggg
     3241 agaggggaac gcgatcacgc tatggcaaag tcctgaaaag gcggaagcca aggctaaaga
     3301 agcgactgcc cgggggatct tggtaacgga acgccgaacg gcaatacgac gcggagtcgt
     3361 acacgcttcc gcctcgaaac acgcgcttcg aacctgaatc cgccccgtcc ggatccgtcc
     3421 ccgtttctgc gctcgtcgtg ccccagttca gacaccactc ccacacgttg ccgtgcatgt
     3481 cgtagagtcc ccaggcattg gggcgtttac tgccgaccag ttgtgcggct gtcggaggcg
     3541 tgggggcact gaacttgtac catcccaacg cattcagatg ctcgttggtg ttcccgttgt
     3601 tattgtcctc ggttccggtg tatttcgctt tcgtgttgcc gtcgttgaac acggtcgtgg
     3661 tcttggcccg gcagacgtac tcccattgcg cctcggtcgg gaggtcgaaa tctccgatac
     3721 cggttttcgc cctcagccta cccataaaag atgtcgcatt cacgattctg ttggatggcc
     3781 aattcaccgc cggatcgtcc gcattcgccg gatcctcgcg gatctcataa taggagaccg
     3841 tgtgtttagc ctgcgtagcc gtaccgcccg tcgtgcccat gacatggtcc cactgcttct
     3901 gcgtgacctc gaagacaccg gcatacatat tctttgtgag ggtcacatta tcggctccgt
     3961 tctgtgtgtc cccctgctgg aaggagcccg ccggtacgcg ccgcagcacc agcttgtcgg
     4021 tcttgtagag gtcgttggtg gtcactccgg tccagatgac cgattccaca atcgcctctc
     4081 ccaccgcatt cgtaatggga ttgcgcatcc acgcgcccca gaggccattc gtgagatcct
     4141 cttcgtaaac atactcgatc tgcttgtccg ctcccgcctc tttcgtcagg tccacgatca
     4201 tgtagagcgg cggcgttaca ggcgtgaggg tcactttgaa ctgtgacaaa acctcttcat
     4261 tggtgtactg gctttgcgtc ggatcccaga cgatccggcg cgcgccctgc ccggaaacac
     4321 cgtaaagatc gccggagagc tcgttgtcgc ccagcacgag cggtatcgag ccgttgtggg
     4381 ccgtcaccgc gatatccacc ccctgcgtcg cattcgcgat cacataatcg atgtccacca
     4441 gcttgctcca cggccaacgc tgacgcacgc tgacatggct gatgtcagcc gcgaggctgt
     4501 tcaacgccag acacgccgcc aaactcgcca atgccgtttt cattgttgtt gtcattgccg
     4561 tttctccatt cttctttctt tgaccgacat gcatcgactt gagacgacct gaaccatccc
     4621 gagtcaaaat cccgatttca aacacacttt agaacttccc gattctcact tcacgctgaa
     4681 tatggacccc tccggcacgc ggaggagcgt ggcctcccac agaccggcgc ttcccggaaa
     4741 atcaagtgcc agatcgaaga taccgtatcc ccatgagccg tcccgcagtt tccagccgta
     4801 gtaaccggcg gcagggagca ggttggtctg cgcgaccccg tcctgacgcc tgatcgacag
     4861 gcggctgttc gaagccgggg cggtcgtgtt gatccagcgg acgtcatgcg ggatcaccgc
     4921 cgcgtcccgt acgtgcgtcc agcgcttatc ggcatcagac agaagcaccg gcgtttcgcc
     4981 atacgcgcct gacaagacac ttagccggga ctgttgcaca cggatcacgg cattttgcgc
     5041 gtcataaaaa cgcagggtca ggtccaccac gtcctcctcc gacggtaccg gggttgcgct
     5101 gacctgccag acccagttgg tcgcggggcg cgtaaagaca acggtctgcg tgccgcgcag
     5161 accatccagc gtcaactccg cccgcacggc actctcgtcc ctccactccc aggacagcag
     5221 caccgtgttg gtgcagaccc tcgaccagtg gccatcacgc gcgggcgagg tgttgatcat
     5281 taccggcacc gatgaggcgg cttgggccga ctggccgacc agcagcaggg ccaccgcaac
     5341 cgttatcgct atgttgccta cgctcatgtc ccctccctcc aatcatcatt gttgataata
     5401 gtaaaccgca tccccagaag cgtgtcaagc catcgagttg atttcagttc tgctcttttt
     5461 tttctcacgc ggaggcgcga agggtacgag gttttttgag ggggacgttg tcccgaaccg
     5521 tcgaaaacac cgcaccccgc caacacacgt ccggcctacg cctgcgcccc cccccgacgg
     5581 gccgaggcga gggtagcgct acagcgttat ggcaaagtta tcacaacgcg gaagccgtag
     5641 tctcggcagg tcgaacccgc tgatccccgt cttggcccgg acgcacccca tgaaactgga
     5701 cggccgaacc gcggaacccg tcgacggcca gtccaccacc gggctgccgt ttgtcgcccc
     5761 ccggaacgga ttgaatatag ccaatttttt cggttatttt ttgaaaaccg cttttttaag
     5821 cacggtccag ggcaggccgt acacggtaag atccgccata aaggggtcgg gatcgaactg
     5881 ctccatgtta aaaaccccct taccccgcca ttgaccggtg agcatcattt tcgcgccgat
     5941 catggccggc acccccgtgg tgtacgagat cgcctgagac tggacctcgc ggtaacactc
     6001 ctcgtgatcg cagatgttgt agataaacac cgtcttgcgt tggccgcggg cgttccgtcc
     6061 ccggacctga cagccgatgc aggtcttgcc tttcgtcagc ggaccgagcg aggcggggtc
     6121 tggcagcaac gccttaagga actggagcgg cacgatatcg accccgttga accgcacggg
     6181 gtcgatccgc gtcatgccga cattcctcaa cacctccaga tgcttgaggt agttgtcaga
     6241 gaaactcatc cagaaccgcg cgcgccgtaa ctccgggatg tgccgggcca gagactccag
     6301 ctcctcgtga tacatcaggt agaccttcag cgaaccgagg ttttcaggca tcgtgaagga
     6361 ggaacacacc gaaagcggtt cggtctcctt ccactcgcca tcctcccaga aacggccctt
     6421 ggccgtaacc tcgcggatat tgatctcggg gttgaaattg gttgcgaacg gttgaccgtg
     6481 actgccggcg ttgcagtcga tgatgtcgat ttcctcgaca tgcggcacga aatgtttacg
     6541 ggcgtaggtg cagaacacac tcgtgacgcc ggggtcgaac cctgaaccga gcagagccat
     6601 gagaccggct tttttgaagc ggctatgata ggcccattgc cacttgtact caaatttcgc
     6661 ctgatccggc ggctcgtagt tggcggtatc cagatagttc acgcccacct caagacaggc
     6721 gtccatgatg tgcaggtctt gatagggcag cgccacgttg atcacgagat ccggccggtg
     6781 ccgcttcagc agccggaccg tctgcggcac gttgtccgca tccacctgcg cggtggtgac
     6841 cttgcgcggc aaccgtgcgg ccagcgcatc cacccgggcg cgggttcgtg aagcgatcac
     6901 gatgtcggta aaaacctcgg gaacctgcgc gcacttgtgc gccacgaccg cgccgacacc
     6961 gccggcgccg ataatcagta ctttggccat gagatcatcc ttttgtggtt gggaatgggg
     7021 agagtataca ccatttggag cgggaggtca tcccaatcaa caaggcgatc cttttcactg
     7081 ggaaaggtgc ccgcgaaaag gttttcggat tcgaggcttg catgccgggc cgggtttgga
     7141 tacaaaagag caaagtgaag gtccttttcc tgttcatcga tggcgtggga ctgcgcccac
     7201 ccgcaacgga caatccggtc cattccggca tctgcccgac gctgtgccat cttatcgaac
     7261 agcacggggt gtcgatcgac gcgtgtctgg aggtgacagg cttgccgcaa agcgccaccg
     7321 gacagacggc cctgttcacg ggtgtcaatg cctcgcagat catgggacgt cattgtgaag
     7381 gctttcccgg tcccacgctc cgcaaactca tcgagcaggg caatctgttc atggatctct
     7441 cgcgcaaggg gctgcgctgc cgatttgcgg acgcctatct ggtagacagc gtggacgaac
     7501 tgcggggccg ccgtttcaaa tccgtaacaa ccgtgatggc gctgacccag cccgaaacga
     7561 tctccattca ggatgacctg atggcgaatc aggcggtctt ccatgacatc acccgtaaga
     7621 gtctgcaaga aaaagggttg gccattccgc tggtcacccc gccgcaggcc gccgaacacc
     7681 tcatcgaggt cgcgttggcg aatgacttca ccctgttcga gtttttccag acggatctgg
     7741 ccggccactc atgccaatac gatcaggcat gcgaaacact ccgcacgctg gatctctttc
     7801 tcgacatggc cgtgagactc tgcaagacca ccggtttgct gctgatcctc accagcgacc
     7861 acggcaatat cgaagacatg ggcacacgcg gccacacccg caatccggtg ccgctgatcg
     7921 cccttggccc cggcgcggac gaaatcaaga acctcgcgtc cagtctaacg gacatcactc
     7981 cgcgcctgac gcgtctgatg gttccgggac acacctccat gccgcggaca gccccccccg
     8041 ctgattccgc ccagtaaacg gcgacttgcg acagtcggcc cgcgttcgta tcgggcagcg
     8101 acgcccgcgc ccccgactcc tgtcttgcgt ctcttgtctc ccgctctttt aggctctcaa
     8161 tcgatccgca ccccggcctg gcgcaacacc cgcttgacgg catggaccgc cttggccgtt
     8221 tctttttcct tcaatctctc ggcgcacagc agcagatcgc cgagacggcc gttcatcacc
     8281 agcggcggac gggtgccgcc cggctcggcc gactttccgt tcgcgtcata aaacgcctcg
     8341 atctcctcct tgaagcggta ggcgctcgga gtcttcttct gtttgtcagt ccaactcaat
     8401 cccagcgcct gcaaccgcag cgcgaaacgc tgcgcggaaa cgccataccg ttttttcaga
     8461 cgcaacagca gttcccatgt ccatccgaca ggcgacagcc ccaattgcca tacggaggcc
     8521 tgcaccgcac gtgcgggcat taggaaaaac gccgcgaacc ggcgcgcgaa ctccgcttcc
     8581 gataacgttt cggcaccgtt ctcgggcaat ggtccgccgg aaccctgccc ctctccggtt
     8641 tcccggacat accagcagac ccgccccagc tcaaacgcca gacggaacaa ctgaagctcc
     8701 ggctgctttt tcaagcggct gttgacgaag atcaacgcgt tacggttctg cgggtcgtat
     8761 ccactgaagg tcggacaccc ttccggcatg tccaaaaaga tcacacgcac tccggcgctt
     8821 tcaagcgctt ccaaataacc gtgcgtcacc gtctcgccga tgcccaaatc ctgccgcaac
     8881 cggccggcca aatattccgc tccccgttcc gtgggcgcga acggtatcgt gagcgcgagt
     8941 gaggaggttt ttaccacgcc gcacaacgtc tccaacttca gcagagcctg cgcgaccgat
     9001 aggcagatga tgtgggccga tttcaacggt tccgcgcccg cgttgatccc gttgtccatc
     9061 ggaggcagca tcaccggctg tttgccatcc ctcgccgccg cgctctcttc ccacacgttc
     9121 tgttcttgga acagatcgtc aatgcggaca ttcaactgct tggccaaccg gtacagaacg
     9181 cgtcccgaag gcaggtgctt gcccttctca atccagccca acggaccggc acggataccg
     9241 atttccgtgg ccagtttaat ttgagaaata cgctgaatct cacgtaactg acggatcctc
     9301 gctccaaccc gcaaggcgat ttcatgttcc acctgcgttt tctgctttcc catacggaac
     9361 ctttctcttg tatgttcgca ccttattccg ccgcgttcac cactattctg ccgtgcaacc
     9421 gcatgccgta agggtattac gcacccgcta ccgcaaacga ctcattcacc aaagtgatta
     9481 tattgttgca gttcaaggtg atccgcgcaa ggggaatcat ctaaaaaaac aagaatatgg
     9541 gattttttac agatggagcg taggaaaaaa acttatgccc gaaccgctgt tcccgtcaca
     9601 aacgcatagg cgtttcggaa gagccgcatc cacgggccgg cctcgccggt gaagagatcg
     9661 gcgggacggt acgagagctg caccgaacgg aagccgcgct ccggatgcgg catcaggatg
     9721 gtcgcacggc cgtcacttga agtaaagccg gtcagcccgc ccttcgtgcc gctcggattc
     9781 cacggatagc gctcggtcgg ctggccgcat ccgtccacat agcgcaacgc tgcaatcgcc
     9841 tgcgcttcct cttccggcga cgcgaagacg gccaagccct cgccgtgcgc cgccgcgacc
     9901 ggcagacgcg aaccctccat gcccttcagc agcacagagg gcgactccat gacc
//
LOCUS       DAOSLE010000096         9900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6606_length_9900_cov_5.868360, whole genome shotgun sequence.
ACCESSION   DAOSLE010000096 DAOSLE010000000
VERSION     DAOSLE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9900
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6606_length_9900_cov_5.868360"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3466
                     /gene="drmA"
                     /locus_tag="PK576_07435"
     CDS             <1..3466
                     /gene="drmA"
                     /locus_tag="PK576_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DISARM system helicase DrmA"
                     /protein_id="HOE61462.1"
                     /translation="NVRTKLVEAIRLDLVGPDNDHPFAAELLPESPSRWYLTGFLVPT
                     EAPLEQKFDETSAEEIDSPAEAGGMDDSVNTDRPAANRRSLLPSSIGLSVLVPKDTRK
                     LDATVVWGDYVEEEPDGETAPGEGGVPPASDGDMPGEVKERSHDGGKPRHWRRTPRTE
                     HVAIELPEPGQRPRRLTVPDSGGLKLVVTVREAGTSVLPAGTRAVSVFLVNYRTPDAL
                     QPYRAFIFQAGIRLECREAFVPRPDPRGWEPDEEWDERVADLQYRDSVEHVVGHGVSG
                     HAEVGDDGACRVVESVWIPQGEVERIAPSSIPDVELGMEVLAELADGADAKAKLGKLV
                     TRYKEWIGRQRSGLSGLNANRTQTANDMLVEAANSADRIAAGIDLLDDPDVLTAFRLA
                     NKAVARSLRQRFGQIQGKAPKAVDAPRWRPFQLAYLLMTMRGVVNPKHGDRDVVDLIF
                     FPTGGGKTEAYLALAAFTLVLRRLRSRDANGKPTIQGAGVSVLMRYTLRLLTLDQLGR
                     AASLICALELERQAHPELLGEWPFEIGLWVGQAATPNRMGHRNDRSAQAPYTAYTKTK
                     QFSRDDSKPSPIPLEECPWCGTKFTRNSFRLVPNENKPLDLRIHCTNEHCEFCGDRYL
                     PVVAVDEPIYRRLPCFMIATVDKFAALPWTGQTGTLFGKVSRFDKDGFYGPCDPGKGV
                     PLPGGCLPPPDLIIQDELHLISGPLGTVAGLYETAIDALATTALDEMSIRPKVVASTA
                     TVRRADSQIRALFNRSRVSVFPPPGPDRRDSFFAHTQHAHEEPPRLYIGIAAQGRSLK
                     VVLLRAGLALLSAGQTAWDEAGDNSDQNPADPYMTLLGYFNSLRELGGSRRIIEDELR
                     SRLAEYGQRRRIQPVDRLFSNRIIAYEVLELTSRVPTNDVAAAKRRLALPFASDERVD
                     VALATNMISVGLDITRLGLMVVLGQPKTSSEYIQATSRVGRDSKRPGLVVTLLNIHKP
                     RDRSHYERFAVYHSHFYRNVEATSVTPFSPRALDRALPAVLVALCRQIIPDLTPARAA
                     KDILTLRDRLGPLCQLIAARAENHNGDLSPADRTALAAKVLNRCNSLLDDWLWIAKQA
                     QDEGSGLQYQREETSPPRRLLYDFLDPEVPNLAPAQRRFRANRSMRDVEASVDIRVKN
                     LNDWGAKP"
     gene            3463..5271
                     /locus_tag="PK576_07440"
     CDS             3463..5271
                     /locus_tag="PK576_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOE61463.1"
                     /translation="MKNPQIRSSQLITTFGPGAMVDLPKDAVIIAGLDNWHYSRRLPL
                     PLVSEPRLQRKLADIIGLPTVALRKPPPPIEERGFTPDIIGYRFPEWFIVQKAVIRNR
                     GGRICRARRLVNLKDLDGGRYRDRDGKKYPVVPVRFVRACRRGHVGDIDWWTFVHGDD
                     RDCRRGLYMEERGTSGDLDSVWITCECGAERPMSHAARMELKALGHCNGSRPWLGPGS
                     REACGDQNRLLIRSASNAYFPQLLSVISIPDSLTAVDQAVAALWESHFSAVATEAQLA
                     FVMTMPAVKQQLDGFTPEQVWTVIERRRRSGDEDERPIKEVEFEVLAQAKDEIGEDKP
                     EGDFYARTLPSAVWADAWTDVIERVVLVHRLREVVAQLGFTRFDAAGPDITGELDIDV
                     QRAPLAIDCSWLPTIENRGEGVFIQFKPEAIAAWLDQPAVQQRDKALQSGFDQWLGEH
                     ENSKHKYPGLPYYMLHSFSHLLLIAMSLECGYPASSIRERVYAMPSQYGVLVYTGSPD
                     AEGTLGGLVEAGRRIKFHIGRALDMGMLCSNDPVCAFHSPRSQDHQPLLGSACHGCLL
                     ISETSCEQRNNFLDRALVVPTVEQLGTEFFRTIPHA"
     gene            5264..6070
                     /gene="drmC"
                     /locus_tag="PK576_07445"
     CDS             5264..6070
                     /gene="drmC"
                     /locus_tag="PK576_07445"
                     /inference="COORDINATES: protein motif:HMM:NF038319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system phospholipase D-like protein DrmC"
                     /protein_id="HOE61464.1"
                     /translation="MRDVFHKLTSGSLCQLAASLRGGELSRGITTHAVRQIAGGVLVG
                     DLAIALTALREDGWLPSQIAELAETVVESRARVAQPEELFDLVLSGPDVSGVPTRETA
                     AVIHALIEEAQREVIVVGYVIHNGKKLFERLAEKAGETPGLDLWLCFNIQRPYKDTSL
                     SSEIVRRFANKFAKDEWPWRPLPPVYYDPRLLEDGTTQSSLHAKCLIIDRKAALITSA
                     NFTEAAHKRNIEAGVIVRYEPLVIRIATYFDTLRQTLFAPVDFSCHQGCP"
     gene            6228..9713
                     /locus_tag="PK576_07450"
     CDS             6228..9713
                     /locus_tag="PK576_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61465.1"
                     /translation="MTARDVFWMTGNAICCVVLHTVAADGSWTNRVGGKWNEPGNWLG
                     GVVAEGEGNTAFFTNAPSAKCLVRVPSVGVTLQNLLFSGFSNAFYLFDGFVTLEGGNP
                     QVGVTNPNDSNNANNYNTDLRGTNGVSFYGGTLGMDTSYTGPTRLCGKNKTLNTFMHF
                     YVAERGVAPSNFLSGGTLIFDGGHLRLEGCKAKAGGTGVFTLTEGDTLIHWKSGTTPW
                     AGFPVSGNGVPDGAFCRQQIHDQAYILSHPATASGDFSLSIVRNSGSLAYQWFDQIDM
                     QAYGILTIVVSGYETQIETDTIAGRERFFRIIGSGNNSTNLAIFRMRGANGFPGAIEA
                     SAVKVEALAQTNGVNPEIPDLTVAATTILTVPEPEAVLSVPGVTLIEGELAKEGAGTL
                     ALQHVYGADPRVCVRGGTLALAAPVVPEPAPVGGAWFHADASATASLVTEQENGTNFV
                     VCWRDVRGNGIYATNDVANSGPRPFLRSACQNGLPVVDFGSYHYPAKGVVGYGAYLNW
                     SEMWTKIYECYFVYSDTEDTASLSAGTYWPTPVGNSNHNAFFARGVNGAILGGPLAQE
                     EVLSGVISLDHATVPAATVVPSGFHVVSFVATNDVRAGAFARERVNSYGGVRIAEVLV
                     FTNRLTVAARREITDYLSRKWFGREPGIGALTLDNGSAVRVADGETVRVGALCVGKPA
                     VKTGGGVLSAETVSGDSLVISNGTVRQTGAAGLLSGGVSCVGSATLDVADEAGSVTAG
                     WIDAEGELSKAGEGELTVATLGVGVTSLHVRGGALNVTGVDPAYGQDAVFHVDADDAA
                     SLETVAVNGTNFVTRWNDCRTNGFYAAAQDTVHRPFLSPGQQNGRSVVDFGAFTKDTN
                     AVYTAWLNWKEYSHSVRDVFVVVRDTDPSKQATLVGNQNHYLDFMRGGSGALVGEHPH
                     SEPIRNGSCAIDGTSVTYTARLPDGFHVVSFFPTNTVRASAFGRERFNGFGGHQLAEA
                     LFFTRELSANERQNIHAQLLSKWLGQTLHRAFDHILVENGGVCSITNMTLATDLLEGT
                     GTIRAQGLTADALSAGTTETAIGSLAVSGGLTLPDGAVVDVDCNAFACDRVDVQGTLT
                     IGGGGTVRLRGDGGDIGEKVFTLFSFDTCAGGENLTLWQVTGEITARYVVRLWIEDNQ
                     IRLGVKGKGTQVLLR"
BASE COUNT         1849 a         2830 c         3317 g         1904 t
ORIGIN      
        1 gaacgtccgg acgaaacttg tcgaagcgat ccggcttgac ctcgtcgggc cggacaacga
       61 tcatccgttc gctgcggaac tgctgcccga atcgccttcg cgctggtatc tgaccgggtt
      121 ccttgtgccc accgaagcgc cgctcgagca gaagttcgac gagacgtccg ccgaggagat
      181 cgactccccg gcggaggcgg gcggtatgga tgactcggtg aacaccgacc gtccggctgc
      241 gaaccgccga agtctgctcc cgtcgtccat cgggctgagc gtgctggtgc cgaaagacac
      301 acgcaagctg gatgcgaccg tcgtctgggg ggactacgtc gaagaggagc ctgatggcga
      361 aacggcaccg ggggagggcg gcgtgccgcc cgcatcggac ggggatatgc ccggcgaggt
      421 gaaagagcgg tcgcatgacg gcggaaaacc caggcactgg cgtcgcactc cccgcacgga
      481 gcatgtggcg atcgagctgc ccgagcccgg gcagagaccg cggcgtctga cggttcccga
      541 cagcggcggg ctgaagttgg tggtgacggt gcgcgaagcc ggaacgtccg tgcttcccgc
      601 cggtacccgt gcggtctctg tatttctggt caattaccgt acaccggacg cgttgcagcc
      661 atatcgcgcc tttatcttcc aagcgggcat ccggctggag tgtcgggaag cctttgtccc
      721 gcgccccgat ccgcgcggct gggagcctga cgaagagtgg gacgagcgcg ttgccgacct
      781 ccagtaccgc gactcggtcg aacatgtggt cggccatggc gtctctgggc atgccgaggt
      841 cggtgacgac ggcgcttgcc gtgttgtgga gtcggtctgg attccccagg gtgaggtcga
      901 gcgcattgcc ccgtccagca ttccggacgt ggaactgggt atggaagttt tggcggaatt
      961 ggccgatggt gccgacgcca aggccaaact cggcaagctc gtcacgcgct acaaggagtg
     1021 gatcgggcgg cagcgctccg gtctttcggg gctcaatgcg aaccggacgc agacggccaa
     1081 cgacatgctg gtggaagcgg cgaacagtgc cgaccgcatt gcggcgggga ttgacctgct
     1141 ggacgatcct gacgtcctga cggcgttccg cctggcgaac aaggcggtgg cacggtcatt
     1201 gcgccagcgc ttcgggcaga ttcagggcaa agcgcccaag gcggttgacg cgccccggtg
     1261 gcgtccgttc cagttggcgt acctgctgat gacgatgcgc ggtgtcgtga atccgaagca
     1321 tggcgaccgc gatgtggtgg acttgatctt cttccccacc ggcggcggca agacggaggc
     1381 ctatctcgcg ctggccgcgt tcaccctcgt gctgcgccgc ctcagatcgc gggacgccaa
     1441 cggcaaaccg accatccaag gcgcgggcgt gagtgtcctc atgcggtaca cgctgcgtct
     1501 gctgacgctt gaccagctcg gccgcgcggc gtcgctcatc tgtgcgctcg aactggaacg
     1561 gcaggcccat cccgagctgc tcggcgagtg gccgttcgag atcgggctct gggtggggca
     1621 ggcggccacg cccaaccgca tggggcaccg caacgaccgc agtgcgcagg cgccctatac
     1681 ggcatacaca aaaaccaaac agtttagcag agacgacagc aagccgtcgc cgattccgct
     1741 tgaagagtgt ccctggtgcg gcaccaagtt cacgcgcaac tccttccggc tggtgcccaa
     1801 tgaaaacaag ccgttggatc tgcggattca ctgcaccaat gagcattgcg agttctgtgg
     1861 cgaccgctat ttgccggttg tggcggtgga tgagccgatc taccggcgac tgccctgctt
     1921 catgattgcc accgtcgaca aattcgcggc gctgccgtgg acggggcaga ccgggacgct
     1981 gttcggcaag gtcagccgct ttgacaagga tgggttctac gggccatgcg atcccggcaa
     2041 aggggtgccg ctgccaggcg gatgccttcc cccgccggac ctcatcatcc aagacgagct
     2101 gcacctgatc tccggtcctc tcggaacggt tgccgggctg tacgagacgg ccatcgacgc
     2161 gctggcgacc actgccttgg acgagatgtc gatacgcccc aaggttgtgg ccagcaccgc
     2221 gacggtgcgc cgtgcagaca gtcaaatccg ggcgctgttc aatcgcagcc gcgtcagcgt
     2281 gtttccgccg ccggggcctg acagacgcga ctcattcttt gctcacacac agcatgcgca
     2341 cgaagagccg ccgcgtctgt acatcggtat cgccgcccag ggacgcagct tgaaagtggt
     2401 gttgctgcgg gcaggcctgg ccttgctgag tgccggccag accgcctggg atgaagccgg
     2461 ggacaattca gatcaaaacc ctgccgatcc ctacatgacg ctcctcgggt actttaactc
     2521 gctgcgggaa cttgggggca gccgccgcat catcgaagat gaactgcggt cgcggctggc
     2581 ggagtatggg cagcggcggc gtatccaacc tgtggaccgg ctgttcagca accgcatcat
     2641 cgcttacgag gtgctcgagt tgacctcgcg tgtgcccacc aacgatgtgg cggcggccaa
     2701 gcgccgtctg gcactgccct ttgccagcga cgagcgcgtg gatgtcgccc ttgccaccaa
     2761 catgatctcg gtgggcttgg acatcacccg gctcgggctg atggtggtgc tcggccagcc
     2821 caagaccagt tcggagtaca ttcaggcgac aagccgcgtc ggacgcgact caaagcgtcc
     2881 ggggttggtg gtgaccttgc tcaatatcca taagccgcgc gaccgttccc actatgagcg
     2941 ttttgcagtc taccacagcc acttctatcg gaatgttgaa gcgaccagcg tcactccgtt
     3001 ctccccgagg gcgctggatc gcgcactgcc tgcggtgctc gtggcgctgt gtcgccagat
     3061 catccccgat ctcacgccag cccgggcggc aaaggatatc ctgacgctgc gcgatcgtct
     3121 ggggccgtta tgccaactga tcgcggcccg tgcggagaat cacaatggcg acttgagccc
     3181 tgcggatcgc acggcgctgg cagccaaagt gctgaaccgc tgcaacagcc ttctggacga
     3241 ttggctgtgg attgccaaac aggcgcagga cgagggctct gggttgcaat atcaacggga
     3301 ggagacatca ccaccgcgac gactcttgta tgactttctt gatccggagg tgccgaactt
     3361 ggctccagcc caacggcgat tccgcgccaa ccgctcgatg cgggatgtcg aagcctcggt
     3421 ggatattcga gttaaaaacc tcaatgactg gggagccaaa ccatgaagaa cccccagata
     3481 cgttccagcc agcttatcac gacctttggc cccggtgcga tggtcgatct gccgaaagac
     3541 gctgtgatca tcgcgggcct ggataactgg cattacagta ggcggttacc gttgccgctg
     3601 gtctccgagc cacggctgca gcgaaagctt gccgatatca tcgggttgcc cactgtggcc
     3661 ctgcgtaagc cgccgccgcc catcgaggaa cgggggttca cgcccgacat catcggctat
     3721 cgcttcccgg agtggttcat tgttcaaaag gccgtgatcc ggaacagggg cggacggatc
     3781 tgccgtgccc gccggctagt taatctcaag gatctggatg ggggccgcta ccgggaccgg
     3841 gacggcaaga agtacccggt ggttccggtg cgttttgtac gcgcctgcag gcgggggcac
     3901 gtcggcgaca tcgactggtg gacctttgtc cacggcgatg accgggactg ccgccgtgga
     3961 ttgtacatgg aggagcgtgg caccagcggt gacctcgact cggtatggat cacctgcgaa
     4021 tgcggtgcgg aacggccgat gagccatgcc gcgcgaatgg aattgaaggc gctcgggcat
     4081 tgcaatggat cccgcccgtg gctggggccg ggctcgcgtg aggcgtgcgg tgaccagaat
     4141 cgcctgttga tccgcagcgc cagtaacgct tattttccgc agttgctctc cgtgatctca
     4201 atcccggatt cgctgacggc ggtggatcag gcagtcgcgg cactgtggga gtcacacttc
     4261 tcggcggtag cgacagaagc ccaattggct tttgtcatga cgatgcctgc cgtcaaacag
     4321 caactcgatg gatttacgcc ggagcaggtc tggacggtta tcgagcgacg caggaggagc
     4381 ggcgacgagg atgaacgccc gattaaggag gttgagtttg aggtgctggc acaggcaaag
     4441 gacgagatcg gagaagacaa gccggaaggc gacttctatg cccggacgtt gccgagtgcg
     4501 gtatgggcgg atgcctggac ggatgtgatt gagcgcgttg tgctcgtcca ccggttgcgg
     4561 gaggttgtgg ctcagcttgg gtttacacgg ttcgatgcag ccgggcccga catcacaggt
     4621 gaactggaca ttgatgttca gcgcgcgcca ttggcgatag actgctcttg gctcccgacc
     4681 atagagaacc gtggagaggg ggtgttcatc cagttcaagc cggaggccat agcggcatgg
     4741 ctggatcagc ccgccgttca gcaacgggac aaggcgcttc aaagcggctt cgatcaatgg
     4801 ctgggcgagc acgaaaacag taaacacaag tatcccgggc tgccttacta catgttgcat
     4861 tcattctccc acctactgtt gatcgcgatg tcactggagt gcgggtatcc ggcctcatcc
     4921 atccgggaac gggtatacgc gatgcccagt cagtatgggg tgttggtcta caccggctct
     4981 ccggatgccg aagggacgct cggcggtctt gtggaagcgg gacgccggat caaattccac
     5041 atcggacggg cgctggacat ggggatgctc tgttcaaacg acccggtgtg tgcattccat
     5101 tctccgcgct cgcaggacca ccagcccctg ctgggcagcg cgtgccacgg atgtctgttg
     5161 atttcggaga catcctgcga acagcgcaac aacttcctcg accgtgcctt ggtggtcccc
     5221 accgtagaac agcttggcac cgaatttttc agaacgatac cccatgcgtg atgtcttcca
     5281 taagctcacc tccggcagcc tctgccagtt ggccgcttcg ctgcggggtg gcgagttgtc
     5341 cagaggtatc acgacccatg ccgtaaggca gatcgcgggc ggggttttgg tcggggattt
     5401 ggccattgca ctgaccgcct tgcgtgagga tggctggttg ccatctcaga ttgccgagtt
     5461 ggccgagact gtcgtcgaga gccgggcgcg ggtggcacaa ccggaagagt tgttcgacct
     5521 tgtcctgtca ggtccggacg tttcaggagt gccgacacgc gaaaccgctg ccgtcataca
     5581 tgcgctgatc gaggaggcgc aacgagaggt catcgtggtt ggatacgtta ttcataatgg
     5641 caagaaactc tttgaacggc ttgcggaaaa agccggagag acgccgggac tggatctctg
     5701 gctctgcttc aatatccagc gtccctacaa agacacgtca ctctcctccg agatcgtgcg
     5761 ccggttcgcc aataagtttg cgaaagacga atggccgtgg cgaccgttgc ctcccgtcta
     5821 ttacgatccc cgtttactgg aagacgggac gacgcagagc agtctgcatg cgaagtgtct
     5881 gatcatcgac cgcaaggcgg ctctcatcac ttccgccaac ttcaccgagg ccgcacataa
     5941 acgaaatatc gaggccgggg tgattgtccg ctacgagcct ttggtcatac ggattgccac
     6001 ctatttcgat acgcttcgcc agacgctgtt cgcccccgtg gacttctcat gtcatcaagg
     6061 gtgcccatga ccatctcggt tgccatctgt aaaacgacca gtggcgatga cataccacag
     6121 tgaatccgct ggacgttcag gtgcgaaaaa gctaaactgc gttttagtta gagttgtttc
     6181 ggtccggagg ccggataggg gatgtggcac atattcagga ggcgcggatg acggcaagag
     6241 atgtgttctg gatgacgggc aacgcgatat gttgtgtggt cttgcacact gttgccgcgg
     6301 acggcagttg gacgaaccgg gtcggcggga agtggaatga gcccggcaat tggttgggtg
     6361 gcgttgtcgc ggaaggggag ggcaacacag cgttcttcac gaacgcgccg tccgccaaat
     6421 gcctcgtcag ggtgccgtct gtcggagtca cgctgcagaa tctgctgttt tccggattct
     6481 caaacgcctt ctatttgttt gacgggttcg tcacgctgga ggggggaaac cctcaagtcg
     6541 gagtcacgaa cccgaatgac agcaacaatg ccaataatta caacacagac ctccgcggca
     6601 cgaacggggt gtcgttttac ggcggcacct tgggcatgga cacgtcgtat acggggccga
     6661 cccgtctgtg cggcaaaaac aaaacgctca acaccttcat gcacttctat gtggccgagc
     6721 gtggcgtggc ccccagcaac tttctctccg gcgggacgct gatctttgac ggcggtcatt
     6781 tgcggctgga ggggtgcaag gctaaggcgg gtggcacagg cgtgttcact ctgacggagg
     6841 gcgacaccct gatccactgg aaatcgggga caacgccgtg ggcgggattt ccggtctccg
     6901 gaaatggggt tcccgacgga gcgttctgca ggcaacagat ccatgatcag gcctatatac
     6961 tcagccaccc cgccacggca tccggtgatt tctctctctc gatcgtcagg aacagcggca
     7021 gtctcgccta tcagtggttt gatcagatcg acatgcaggc atacgggatc ctcaccatcg
     7081 tggtgagcgg gtatgagacg cagatcgaaa ccgacaccat cgccggtcgg gaacggtttt
     7141 tccggatcat cgggagcggg aacaacagta ccaatcttgc gatctttcgc atgcggggag
     7201 cgaacgggtt ccccggagcg atcgaggctt ccgcagtcaa ggtcgaggcg ctggcgcaga
     7261 ccaacggtgt gaaccccgaa attcccgacc tgactgttgc cgccaccacg atcctgacgg
     7321 tgccggagcc ggaggcagtc ctgtccgtgc cgggtgtgac ccttatcgaa ggagagttgg
     7381 cgaaagaggg tgcggggact ctggcgctgc agcacgttta cggggccgat ccccgtgtgt
     7441 gtgtccgcgg tggcacgctg gcgctcgctg ctccagtggt tccggagcct gcgccggtcg
     7501 gcggggcatg gttccacgcg gatgcgagtg caacggccag cctggtgacc gaacaggaga
     7561 acgggacgaa tttcgtggtc tgctggcggg atgtccgggg caacggcatc tacgccacga
     7621 acgatgtcgc caacagcggg ccgcgtccgt ttctccggtc cgcgtgccag aacggattgc
     7681 cggtcgtgga tttcggttcg taccactatc cggcgaaagg ggtggtgggt tatggcgcgt
     7741 atctgaactg gtccgagatg tggacgaaga tttacgagtg ttatttcgtt tacagcgata
     7801 ccgaggacac ggcctcgctg tccgccggga cgtactggcc cacgccggtc gggaactcga
     7861 atcataatgc ttttttcgcg cggggggtca acggggcgat tctgggcggt ccgctggcac
     7921 aggaagaggt cttgtccgga gtgatctcgc tggatcatgc aactgtgcct gcggcaaccg
     7981 tcgtgccttc cggctttcat gtggtgtcgt ttgtcgccac gaacgatgtc cgggccggcg
     8041 cgttcgcgcg tgagcgggtg aacagttatg gcggagtccg gatcgcggag gtgctggtgt
     8101 tcacgaaccg cctcacggtc gcggcgcggc gggagattac ggactacctc agccgcaaat
     8161 ggttcggccg cgagccgggg atcggggcgc tgacattgga taacggaagc gcggtgcggg
     8221 ttgcggacgg cgagaccgtg cgcgtcggcg cgctatgtgt cgggaagcca gccgtgaaaa
     8281 cgggcggcgg ggtgttgtcg gcggagacgg tgagcggcga ttctctggtg atttcgaacg
     8341 ggaccgtgcg tcagacgggc gcggccggtc ttttgtccgg aggcgtcagc tgtgtgggtt
     8401 ccgcaacatt ggatgtggcc gatgaagcgg gatcggttac cgccggatgg atcgatgcgg
     8461 agggggaact gtctaaggcc ggcgagggtg aactgacggt cgcgacgctg ggtgtgggtg
     8521 tgacgtccct gcatgtgcgg ggaggagccc tcaacgttac gggcgtggac cccgcgtacg
     8581 gtcaggatgc ggtctttcat gtggatgcgg acgatgccgc cagtttggag acggttgcgg
     8641 tgaacgggac gaacttcgtg acacgctgga acgattgccg gaccaacggg ttctacgccg
     8701 cggcgcagga cacggtccac cggcccttcc tgagccccgg gcagcagaac ggacgcagtg
     8761 tggtggattt cggcgcgttc accaaagaca cgaatgcggt ttacacggcg tggctcaact
     8821 ggaaggagta cagccattcg gtgcgcgacg tgttcgtggt ggtccgggac accgaccctt
     8881 ccaagcaggc cacgctggtc ggcaaccaga accactatct ggatttcatg cggggcggtt
     8941 ccggcgcgct ggtgggcgag catccgcact cggagccgat ccgcaacggc tcctgcgcga
     9001 tcgacggcac ctccgtgacg tatacggcga ggttgccgga cggattccat gtggtgtcgt
     9061 tcttcccgac caatacggtt cgcgcatcgg cgttcgggcg cgaacggttc aacgggttcg
     9121 gcggacatca actggcggag gcgctctttt tcacccgcga gttgtcggcg aatgagcggc
     9181 agaacattca cgctcaactg ctctccaaat ggctggggca gacattgcat cgcgcgttcg
     9241 accatatcct cgtcgagaac gggggcgtat gctcgatcac gaacatgaca ctcgccacag
     9301 acctgctgga gggaacagga acaatccggg cgcaggggct tacggcggac gcgctttcgg
     9361 cgggcacaac ggaaacggca atcgggagcc tggcggtttc cggcggactg acgctgccgg
     9421 acggggcggt tgtggatgtg gattgcaacg cctttgcctg cgaccgggtg gatgtgcagg
     9481 gcacgctgac tatcggaggc ggcggcaccg tgcggttgcg gggtgacggg ggcgacatag
     9541 gggagaaagt gttcaccctg ttctcgttcg acacttgtgc ggggggcgag aatctgacgt
     9601 tgtggcaggt cacgggcgag atcacggcgc ggtatgtcgt gcggttgtgg atcgaggata
     9661 accagattcg cctcggggtc aagggaaaag ggacgcaggt tctgttgcgt tagtttttca
     9721 ccttgtcgat gacaaggcaa aaacgaaaag gatgggatag gtgtggtccg gcgacaaaga
     9781 tgcggacggt ttttccaagg agccgtgctc agcgcacatg tatacgtttg ccggttggga
     9841 tgcggcaggc gctggcacgc ccgaggatgc gggcgcgtga ttccggaaag ggagcgggcg
//
LOCUS       DAOSLE010000097         9845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6654_length_9845_cov_6.841062, whole genome shotgun sequence.
ACCESSION   DAOSLE010000097 DAOSLE010000000
VERSION     DAOSLE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6654_length_9845_cov_6.841062"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PK576_07455"
     CDS             complement(<1..1042)
                     /locus_tag="PK576_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61466.1"
                     /translation="MKRHLIECGWLAALLCGMVAGAPVAVNGADDSGRAVAKLIEVSR
                     KHPCYFVEGATGKTWIPIGCNICFARLNVSDAEVRANFERWLRRFAANGGNCIRLWAG
                     HRSLEVMPEKPGVWDAEKTKTMKGIVALCEELGIKVKITFESFRMCLSPEVQAKTAQP
                     KVNFDYNGIFNRPLYAPYAKNVAEFFRSPECRAIYLRKAQYFKDLGFADSPAVYCWEL
                     WNEINCCGSIAQYSEWSEWALAELKRMFPRQLTVNNLGSFSDVGAYQMYDALAQEKGN
                     DFMQIHRYLDAGACLDACRASMDVVAASATRELLDRSPKPAIVAEIGAVQANHAGPFP
                     YYPFDKEGTLLHD"
     gene            complement(1167..2303)
                     /locus_tag="PK576_07460"
     CDS             complement(1167..2303)
                     /locus_tag="PK576_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HOE61467.1"
                     /translation="MTIPTANFTHRKVLEGILDYARIKGPWQFHLNTGDIAVQDMKRV
                     DNWKCSGIIALSSNKAVLRRLVRFRKPTVFINPPDAGNVPPRGCVFVRRDDRNLGRVA
                     AEYFISGGFRSFAFVGASRPAAWSDDRRDGFTTRLAQEGFTCIDYGSVTPEESADAAI
                     EAPRLLTWLKTLPRQTALFTVKDIRGQQILSACLDASIRVPEDLAVLSTDNDPVLCDT
                     VSPPLSSISLDGENTGKLCAGILDDLLHGKKVEPVVSIMCPRIITRRSSDVTAVEDPL
                     LARALDYVRAHLDEPFRIGKLASETGISVRTIQLRARKQFGHPFREEIRRLRLAKAIS
                     LLSNTDLPAADIAAMCGFCSGSHLSRAVKDAFGYPPSTFRPKPT"
     gene            complement(2562..2729)
                     /locus_tag="PK576_07465"
     CDS             complement(2562..2729)
                     /locus_tag="PK576_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61468.1"
                     /translation="MFCGETVDPDSDPDPDPDLDETMKPETRNHETLKPDSDPDSDPD
                     PDPDLDETLKP"
     gene            complement(3019..4323)
                     /locus_tag="PK576_07470"
     CDS             complement(3019..4323)
                     /locus_tag="PK576_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002930776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61469.1"
                     /translation="MSNLNFSIDWLSSGSADPVLRDTSAQLSIHLDHVCLTRNEDIWS
                     KTVRDSILVSVYPLASWLAGSWWRLNNEPLPASGTRPPLAWRMAHELGAANQGFVWPR
                     VLFVPDGESMSIWAEPIATPWQSVNYMTGLDTVKTLSMKDFQRSADDLVENVLSRLEA
                     VGQGQTELASLWALVKEDRNDAKASRVRQLEAQMGFDPEECSPAIIEEALSMQATTGQ
                     SAMSELAPVFSDEISRIGDLQGQSGLKGRPLVTSADIDMSAIRARPWEQGVAAAKRLR
                     ERLDNSAAPISDAILLDLLGISQKEAARWVPAKTPVAIAKPGHAGHWDLIPRKAHPIA
                     KRFEWSRFLGDVLSQPEGSADWLVSSDLATARQKRQRAFAAEFLCPIDALVDVLNGDY
                     SETAQEEAAEYFKVSEKTVESHLANHGYIERVEPQLPYRLAV"
     gene            complement(4316..4747)
                     /locus_tag="PK576_07475"
                     /pseudo
     CDS             complement(4316..4747)
                     /locus_tag="PK576_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008318414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4896..5753
                     /locus_tag="PK576_07480"
     CDS             4896..5753
                     /locus_tag="PK576_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61470.1"
                     /translation="MKFSVGYQDDAAWIEAIIARKDVMHDVYFAASGWASGRAVIRSE
                     DRLLDDLGRMAEAGLPLHLLFNATCYGGESLAKSLFSHIGETVERFAADGSLEGVTTT
                     SPLIAKFVKDNFPELKTRASVNMEIGTIEGMEYLADVFDGYYLKRELNRDLDAVRAAK
                     TWCDAYGKELFILANSGCLNHCSAHMFHDNLVAHETEIAQKDNGYVYRGMCWEWLAKP
                     ENRAKWMERTNWIEPESVTKYEGLCTAMKLATRINAHPVRILESYATGSHLGEIKGLL
                     EPDHSALWR"
     gene            5755..6840
                     /locus_tag="PK576_07485"
     CDS             5755..6840
                     /locus_tag="PK576_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61471.1"
                     /translation="MLSAKRIKEIAKKAGADNCGIAPISRMSDAPDEMNPTFLFPNCK
                     SMVGLVFRIPRGVQRGIEEGTQFYQYPSMAYGGINEILAPATLYHVGKAIEDAGYEAF
                     VYRNTGARGCVSDMDGSEDAKLSPEEQIEVSENAKDSTAHHRSVDFTRATRPGENVPP
                     DLQFQFRICAVACGLGEIGWSKMLLTPEFGPLQRVAFLFTDAEFDEYDEIYNGKPLCR
                     RCGACVRECPGNCIPEIKSGKKCTASIGDYKMEWGDIDMWKCYAFYTHAGRYYNPFVP
                     KEVWDENKDGDLDLFAGKTDASEQEIMKVYRALAKYFPSWVGYNMAKCGGCIRACVEM
                     LEKPGGCLHGRFKNPLRTRPKAWKMDR"
     gene            6860..7648
                     /locus_tag="PK576_07490"
     CDS             6860..7648
                     /locus_tag="PK576_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61472.1"
                     /translation="MNRETIEQVARELGAVMFGVGDLKLFEGEDPRHDPKQVCPKAQA
                     IIGFGIPVPKGLYKTMKDESQFYAYTTLGVKAIDEEYFDIFLFKMASIIEDAGYDACL
                     QRTTPNLRVKGDKTANPEMIGVYELAHAEAVEPGKPPPDVMIDFGKAARACGIGMPGR
                     YGRVINREYGPFMRYAFIITDAPLETNAPYADDLCAGCDACVKACDAGCIDVEKGLDS
                     WKCWEHYNKARGYSLPRTQWGYQACLCGRKCDVACWEHIKGVKL"
     gene            7645..8610
                     /locus_tag="PK576_07495"
     CDS             7645..8610
                     /locus_tag="PK576_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61473.1"
                     /translation="MKHELLKLAKECGADLVGVAPASRFPADHKIFRIYPQVKSVIGL
                     AFRCLRGSYRGTEEGTTYYQYTTMSVENMEETVMPIALVRLANRIESKGFTAVPQRRH
                     QTIMEAEDGMNPEVAYDAIARGRVEEPQFDFTDAAVRCGLGERGFHGALLTDDFGPMQ
                     RTCFILTDAELEPDQVVTPHLCDRCGACVKGCPGKCIDPESGAIDPWRCAVYYNGANG
                     TKNPFMPPEAFPDFEDRLAIIAGEAEIDSEKAKRILDAIYFYPPAQHSYTCSICGRAC
                     DVACYCHLEKKGVLKRTFKTPFRTRPEWKFQLDDFAPESVNARQE"
     gene            8620..>9845
                     /locus_tag="PK576_07500"
     CDS             8620..>9845
                     /locus_tag="PK576_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOE61474.1"
                     /translation="MRFALADWLVTVAFFVVLLAIPAFANRRRARTSSDFFQSGRSMP
                     WWLIGISMVAAMTSTNSANLFTQLIRENGLAGNWVWWSFLTGGVLTVFVYAKLWHRSG
                     ATTDIAFYELRYSGKPAAFLRAFRAVYLGVIYNVIVMATVLLGAVKLGAVLFGVPVWT
                     ILAVTAVASIAYSMIGGIRGTIYADFYLFTLIMIGAVAVTAFALSQPEVGGWAALMQN
                     AAIRPKLDFLPRFDDLDTAIAVFIVPVAIQWWNVWYSGSEPGGGGYIVQRMLTAKTPN
                     HALGGTLFAQIVQYALRPWPWYITAFASILVFPDLASIRAAFPDVDPALVGNDMAYPA
                     MIKFVPAGWLGLVAASLMGALFSTIAAHLSMGANYVANDVWVRFVRPQADGRELIRVA
                     RWTSVGLMLAGCLLVP"
BASE COUNT         1996 a         2737 c         2992 g         2120 t
ORIGIN      
        1 cgtcgtgcag cagcgtgccc tccttgtcaa aggggtagta ggggaagggg ccggcatggt
       61 tcgcctgaac ggcgccgatc tcggcgacga tcgcgggctt cggcgagcgg tcgagcagtt
      121 cgcgcgtcgc gctcgcggcc accacgtcca tcgacgcgcg gcaggcatca agacaggcgc
      181 ctgcgtcgag gtagcggtga atctgcatga agtcgttgcc cttctcctgc gcgagcgcgt
      241 catacatctg ataggcaccc acgtcagaga agctgccgag gttgttgacc gtgagttgac
      301 gcgggaacat ccgcttcaac tcggcgagcg cccactcgga ccattccgaa tactgggcga
      361 tcgagccgca gcagttgatc tcgttccaga gttcccaaca gtagaccgca ggcgaatcgg
      421 cgaagccaag gtccttgaaa tactgcgcct tgcggaggta gatcgcgcgg cactccggcg
      481 aacggaagaa ctcggcgacg ttctttgcgt acggcgcgta gagcggacgg ttgaagatgc
      541 cgttgtagtc gaagttcacc ttcggctgtg ccgtcttcgc ctgcacctcg ggcgagagac
      601 acatgcggaa gctttcgaag gttatcttca ccttgatgcc gagttcctcg cagagggcga
      661 cgatgccctt catcgtcttc gtcttctcgg cgtcccacac gccgggcttc tcaggcatca
      721 cctcgagcga gcggtgtccg gcccagaggc ggatgcagtt gccgccgttc gcggcaaagc
      781 gccgcagcca gcgttcgaag ttcgcgcgca cctcggcgtc cgagacgttg agccgggcaa
      841 agcagatgtt gcagccgatc ggaatccatg ttttccccgt ggcgccctcg acgaagtagc
      901 acggatgctt ccgcgagacc tcgatcagct tcgcaactgc acgcccggaa tcatcggcgc
      961 cattcacggc caccggtgcg cccgcaacca tcccgcacag cagcgccgcc agccagccac
     1021 attcaatcaa atgacgtttc attgtttctt ctcgccacat gttataccgc aactcgtggc
     1081 actgtaacac attccagaaa ccggaagaag tccatttcag ccaaaaatca gccctactgt
     1141 taccaaacca aagagctgcg gggatgtcat gtcggtttcg gccggaatgt gcttggcggg
     1201 tatccgaagg cgtcctttac agcgcgcgag agatggcttc cgctacagaa cccgcacatc
     1261 gctgcgatgt cggcggccgg gagatctgtg ttggaaagaa gcgaaatggc cttggcaaga
     1321 cgcaaccgac gaatttcctc acggaagggg tgcccgaact gtttgcgcgc cctgagttgg
     1381 atcgtacgaa ccgaaatccc cgtctcgctt gcgagtttac cgattcggaa gggttcgtca
     1441 agatgggcgc ggacataatc aagcgcccgt gcgaggagcg gatcttcgac ggcggttaca
     1501 tcggaggatc ggcgtgtgat gatgcgtggg cacatgatgc tgacgacagg ttcgactttt
     1561 ttcccatgga gcaggtcgtc gaggattccg gcgcatagtt ttccggtatt ttcgccatcc
     1621 agggaaatcg acgacaaagg cggcgatacc gtgtcgcaga ggactgggtc gttatccgtc
     1681 gagagcacgg cgagatcctc tggaacgcgg attgaggcgt cgaggcacgc ggataatatc
     1741 tgctgcccgc ggatgtcttt tacggtaaaa agtgcggtct gacgcgggag cgtcttgagc
     1801 catgttaaaa gccgcggcgc ttcaatggcg gcgtccgcgc tctcttccgg cgtgacggag
     1861 ccgtagtcga tgcaggtgaa gccttcttgt gcgagccgag ttgtaaatcc gtcccgccga
     1921 tcatctgacc aagccgccgg acggctggca ccgacaaagg cgaaggatcg gaaaccgccg
     1981 gagatgaagt attccgccgc gacacgtccg aggttgcggt cgtcgcgccg gacgaatacg
     2041 cagccgcgcg gcgggacgtt ccccgcgtcc ggcggattga tgaaaaccgt cggcttgcgg
     2101 aaccgcacga gccgtctcag cacagccttg tttgaggaca gggcaatgat gccggagcac
     2161 ttccagttat cgacccgttt catgtcctgt accgcgatgt ctccggtatt gaggtgaaac
     2221 tgccaaggcc ccttgattcg ggcgtagtcg agaatgccct ccaaaacttt ccggtgcgtg
     2281 aaattcgccg tcggaatggt caccagaact ctgggtatac cggatgcagc ggaggcccgt
     2341 ttcatacccg cattcaacca aaattcctga tttggcacaa tcgcatattg taatcggtta
     2401 acctaaaaag agcagttgcc ccttcaactt tcttgggttt cagggtttct gggtttctgg
     2461 tttcagggtt tcatggtttc atggcttcat ccaaatcggg gtcgggatcg ggatcgctat
     2521 cgggatcgct atcgggtttc agggtttcat ggtttcaggt ttcatggttt caaggtttca
     2581 tccaaatcgg ggtcgggatc gggatcgcta tcgggatcgc tatcgggttt cagggtttca
     2641 tggtttctgg tttctggttt catggtttca tccaaatcgg ggtcgggatc gggatcgcta
     2701 tcgggatcga ctgtttcacc acagaacaca accgcctcac agtctcaccg cctcacagtc
     2761 tcacagcctt tgagattgcg ggctccgccc gcatctcaat acgatgcggc ggggaccgcg
     2821 acaaaaccca atgtttgcgg ggctttgcta gaattctacc cgttcaattg ctccttttag
     2881 ggttagtaac agggcatgcg agtgaaagcg aagaacgctc aactttcaag tgcggagcat
     2941 cgggagggcg agcgtccccg cgagccgctt gagcaaaccg ccaagacgcc agaaacgcca
     3001 ggggcggccc cgagagtctt acacggccaa tctatatgga agttgcggct ccacgcgctc
     3061 gatgtaacca tggtttgcca gatgggactc gactgttttt tcgctgacct tgaaatactc
     3121 cgcggcttct tcttgcgcgg tttccgaata gtcgccgttg agcacatcga cgagcgcatc
     3181 aatcggacaa aggaattctg cggcaaacgc gcgttggcgt ttctgacgtg cggttgcaag
     3241 gtcagaggaa accaaccaat ccgcgctccc ctcaggttgg gaaaggacat ccccgagaaa
     3301 acgagaccac tcaaaccgtt tggcaatcgg gtgcgccttt ctgggaatga gatcccagtg
     3361 tcccgcatga ccgggcttgg caatcgccac gggagtcttg gcaggcaccc accgcgccgc
     3421 ttctttttgg gagataccta gcagatcgag cagaatagca tcagaaatag gagccgcact
     3481 attgtctagg cgttctcgaa gccgtttagc cgccgccacg ccttgttccc agggtctcgc
     3541 ccgtatggcg gacatgtcga tgtcggcaga agtcaccagc ggacgcccct ttagtccaga
     3601 ctgcccttgg agatcgccta ttctggaaat ctcatcgcta aagacgggag ccaattctga
     3661 catggctgac tgcccggttg tcgcttgcat gctcagggct tcttcaataa tcgcgggtga
     3721 gcactcttcc gggtcaaacc ccatctgagc ttctaattgt cgtaccctac ttgctttggc
     3781 gtcgtttctg tcttctttta cgagcgccca gagtgaagcc aactccgttt ggccctgtcc
     3841 aaccgcttcc aaccgactta atacgttttc gaccaagtca tcggcagaac gctgaaaatc
     3901 tttcatcgag agcgtcttta cggtgtcgag ccccgtcata tagtttacgg attgccacgg
     3961 cgtcgcgatc ggttccgccc agatgctcat cgattcgccg tcgggaacaa acaagaccct
     4021 gggccacaca aaaccctgat ttgcggctcc caattcatga gccatgcgcc aagcgagagg
     4081 cggcctggtt ccagaggccg gcagcggctc gttgttcaat ctccaccatg aacccgccaa
     4141 ccaggacgct aatgggtaaa ccgacaccaa gatcgagtcg cgtaccgttt tcgaccagat
     4201 atcctcgttg cgagttaggc agacatgatc caaatggatt gacaattgag cggaagtatc
     4261 gcgcagaacg gggtcggcac tccccgacga caaccaatct atggaaaagt ttaggttaga
     4321 catgttgcct ccaccgcgca agtacttcag ccgccaacgg atcgcttttc ggcatcgggt
     4381 agccgtggta acagcctgtc gccggatttt cgagctacgc ttccagcggg atgtcgcttt
     4441 ttgtcacaga ccacacattc ttgggacagc cctgttcttc cctatcacta accaaccctc
     4501 ttcgcatgcc ttcttgaagc aggttcagcg cttcgcgccg ggtgaaaaca tcagcgacat
     4561 cacagagcga tttcccctgt cggggagaca ccggaggtgt cagaccgaag tcacccggat
     4621 tcttcttgtg ttcaggattt cccccgtaac tgaccttttt cgacaggtca tccagacgtt
     4681 tcaacacgtc tgtgtcgacc gcgaggaatc tccgtttggg attgaattgt ctgtaacgcc
     4741 cattcatcaa atcacctgac aaacagcaat caattaaaaa atataccata acgtgctgac
     4801 ttcgcacacc caaagatctc gcatattcgc tttagtgcaa aatataatgc gcatttgtgt
     4861 aaatgaacgt cgatgaattg cggtaagata gacacatgaa attcagtgtg gggtatcagg
     4921 atgatgcggc gtggatcgaa gcgattatcg cgcgtaagga cgtcatgcac gacgtgtact
     4981 tcgcggcttc cggttgggca tcggggcgtg ccgtgatcag gtcggaggac cgtctgctcg
     5041 acgacctcgg ccggatggcg gaagcgggat tgccgctgca cctccttttc aacgcgacct
     5101 gttatggtgg cgagtcgctt gcgaagtcgc ttttttccca tatcggcgaa accgtggaac
     5161 gttttgccgc ggatggatcg cttgagggcg tgacgacgac ttctccgctt atcgcgaagt
     5221 tcgtgaaaga taatttcccg gagctgaaga cgcgtgcatc ggtgaacatg gagatcggaa
     5281 cgatcgaagg tatggagtat cttgctgacg tttttgacgg atactacctg aagcgtgaac
     5341 tcaaccgcga tctcgatgcc gtgcgcgcgg cgaagacgtg gtgcgatgcc tacggcaagg
     5401 agctcttcat ccttgcgaat tccggatgcc tcaaccactg ctcggcccac atgttccacg
     5461 ataatcttgt cgcgcacgaa acggagatcg cacagaagga taacgggtac gtgtaccggg
     5521 gaatgtgctg ggagtggctt gccaaaccgg agaaccgcgc gaagtggatg gaacggacga
     5581 actggattga accggaaagc gtgacgaagt acgaagggct ctgcacggcg atgaagctcg
     5641 ccacgcgcat taacgcgcat cctgtccgta tcctggagtc ctatgcgacc ggatcgcatc
     5701 tcggagagat caaggggctg ctcgaacccg atcattcggc actttggagg taacatgctt
     5761 tcagcaaaac gaatcaagga gattgccaaa aaggccggcg ccgataattg cggcatcgcg
     5821 ccgatttcgc gcatgagcga tgcgccggac gagatgaatc cgacgttcct gttcccgaac
     5881 tgcaaatcga tggttggtct cgtctttcgt atcccgcgcg gcgtacagcg cggcattgag
     5941 gaggggacgc agttctacca gtatccgtcg atggcctacg gcggcatcaa cgagatcctt
     6001 gcgccggcga ccctgtacca tgtcggcaag gcgatcgagg acgcgggcta cgaggcgttt
     6061 gtctaccgca acaccggcgc gcgtggatgt gtcagcgata tggacggatc ggaggacgcg
     6121 aagctctctc ctgaggaaca gatcgaggtg agtgagaacg cgaaggattc gacggcgcat
     6181 caccgttccg ttgacttcac ccgcgcgacg cgtccgggcg agaacgtccc gccggacttg
     6241 cagttccagt tccgcatctg cgcggttgcc tgcggtctcg gcgaaatcgg gtggtcgaag
     6301 atgctgctga cgccggagtt cggccccttg cagcgcgtcg cgtttctgtt tacggacgcc
     6361 gagttcgacg aatatgacga gatctacaac ggcaagccgc tctgccgccg gtgcggggcg
     6421 tgtgtgcgcg agtgcccggg gaattgcatt cctgagatta aatccggcaa gaagtgtacg
     6481 gcttcgatcg gcgactacaa gatggagtgg ggcgatatcg acatgtggaa gtgctatgcc
     6541 ttctatactc acgccggacg ttactacaat ccgttcgttc ccaaagaggt ctgggacgaa
     6601 aacaaggacg gtgaccttga tctgttcgca ggtaaaaccg atgcgagtga acaagagatc
     6661 atgaaggtct accgggcgtt ggcaaagtac ttcccgagct gggtcggcta caatatggcc
     6721 aagtgcggcg gctgtatccg cgcttgtgtc gagatgctcg agaaaccggg cggctgtcta
     6781 cacggccggt ttaagaatcc gctccgtaca cgtccgaagg cgtggaaaat ggacaggtaa
     6841 cgggttgagg tgagggaaga tgaatcgaga aacgattgag caggtggcgc gtgaactcgg
     6901 ggcggttatg ttcggcgtcg gagatctgaa gctgttcgag ggagaggatc cgcgccacga
     6961 tccgaagcag gtctgcccga aggcgcaggc gatcatcggt tttggcattc ccgttccgaa
     7021 gggtctatac aagacgatga aggacgaatc gcagttctat gcgtatacga cactcggcgt
     7081 gaaagcgatt gatgaggaat acttcgatat ttttctcttc aagatggctt cgatcattga
     7141 agacgccggt tatgacgcgt gcctgcaacg gacgacgccg aacctccgcg tcaagggcga
     7201 caagaccgcg aaccccgaga tgatcggcgt ctacgagctt gcccatgcgg aagcggtcga
     7261 gccgggcaag ccgccgccgg acgtcatgat cgacttcggc aaggccgctc gggcgtgcgg
     7321 catcggcatg cccggcaggt acggacgcgt catcaatcgc gagtacggtc cgttcatgcg
     7381 ctatgccttc atcatcaccg acgctccgct tgagacgaac gcgccgtacg cggacgatct
     7441 ctgcgcgggc tgtgatgcct gcgtgaaggc gtgcgatgcc ggatgcatcg acgtggagaa
     7501 gggactcgac tcgtggaagt gctgggagca ttacaacaag gcacgcggtt attcgttgcc
     7561 gcgaacgcag tgggggtatc aagcgtgcct gtgcggacgc aagtgtgatg tcgcctgctg
     7621 ggagcacatc aagggggtga agttgtgaag cacgaacttc tgaagttggc taaagaatgc
     7681 ggtgcggatc tggttggcgt cgcgcctgcg tcgcggttcc cggcggatca caagattttc
     7741 aggatctatc cgcaggtgaa gagtgtcatc ggtttggcgt tccggtgcct tcgcggtagc
     7801 tatcgcggta ccgaggaggg aacgacctac taccagtata cgacgatgtc cgtcgagaac
     7861 atggaggaga cggtgatgcc gatcgcgctt gtgaggctgg cgaatcggat cgagtccaag
     7921 ggcttcacgg ccgtgcccca acgccgccac cagacgatca tggaggcgga ggatggcatg
     7981 aacccggaag tcgcctatga cgcgattgcg cgcggacgcg tcgaggagcc gcagttcgac
     8041 ttcacggatg cggcggtgcg gtgcggactc ggtgaacgcg gcttccacgg tgcgctcctg
     8101 acggacgatt tcgggccgat gcagcggacg tgcttcatct tgacggatgc tgaacttgag
     8161 ccggatcagg tggtgacgcc gcatctttgc gacaggtgcg gcgcatgtgt gaaggggtgt
     8221 ccgggcaaat gtattgaccc agagtctggc gcgattgatc cgtggcgttg cgccgtctat
     8281 tacaacggcg cgaacggcac gaagaatccg ttcatgccgc cggaggcctt ccctgatttc
     8341 gaggaccggc ttgcgatcat cgcgggcgag gccgagatcg attcagagaa agcgaagcgg
     8401 atccttgacg cgatctattt ttatccgccg gcgcagcact cctacacctg ctcgatctgc
     8461 ggacgcgcct gcgatgtcgc atgttattgc catttggaga agaagggggt cctgaaaaga
     8521 acattcaaga cgcccttccg tacacgtccc gagtggaagt tccaactcga cgacttcgca
     8581 ccggaatccg tgaacgctag acaggagtag caacgggtca tgagattcgc actggcagac
     8641 tggttggtga ccgtagcgtt ttttgtcgtg ttgctcgcga taccggcgtt cgccaaccgg
     8701 cgtcgcgcac gtacgtcttc cgactttttc cagtcggggc gttcaatgcc ctggtggttg
     8761 atcggcatct cgatggtggc cgcgatgacg tcgaccaact cggcgaatct gttcacccag
     8821 ttaattcggg aaaacggact cgctggcaac tgggtttggt ggtcgtttct cacgggcggc
     8881 gtcctcacgg tcttcgtcta tgcgaagctc tggcaccggt cgggagcgac gacggacatc
     8941 gcgttctacg aattgcgata ttcggggaag ccggcggcgt ttctccgcgc cttccgcgcc
     9001 gtttatctcg gcgtcatcta caacgtgatc gtgatggcga ccgttcttct cggggcggtg
     9061 aagctggggg ccgttctttt cggtgtgccc gtctggacga tcctcgcggt tacggctgtc
     9121 gccagcatcg cctattcgat gatcggcggc atccgcggaa cgatctacgc cgacttctat
     9181 cttttcacgt tgatcatgat cggcgcggtg gcggtgaccg cattcgcgtt atcgcaacct
     9241 gaggtcggcg gatgggcggc gctgatgcag aacgccgcga tccgtccgaa gctcgacttc
     9301 ctgccgcgat tcgatgatct ggacaccgcc attgcggttt tcatcgtgcc ggtcgcgatt
     9361 cagtggtgga acgtgtggta ttcgggttcg gagccgggcg gcggcggtta catcgtccaa
     9421 cgcatgttga cggcaaagac gccgaaccac gcgctcggcg ggacgctttt cgcgcagatc
     9481 gtacagtacg cgctccgccc gtggccgtgg tatatcaccg ccttcgcctc catccttgtc
     9541 tttcccgacc ttgcgtccat acgcgcggcg tttccggatg tcgatcctgc actcgtcggc
     9601 aacgatatgg cgtatccggc gatgatcaag ttcgtgccgg cgggctggct cggactcgtt
     9661 gcggcgtcct tgatgggggc tttgttctcg acgatcgccg cgcatctctc gatgggcgcg
     9721 aactacgttg ccaacgatgt gtgggtgcgg ttcgtgcgtc cgcaggcgga cgggcgggaa
     9781 ctgatccgcg tcgcgcgctg gacctcggtt gggctgatgc tcgcgggctg tctgctggtg
     9841 ccgtt
//
LOCUS       DAOSLE010000098         9839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6660_length_9839_cov_8.029538, whole genome shotgun sequence.
ACCESSION   DAOSLE010000098 DAOSLE010000000
VERSION     DAOSLE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6660_length_9839_cov_8.029538"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1548
                     /locus_tag="PK576_07505"
     CDS             286..1548
                     /locus_tag="PK576_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOE61475.1"
                     /translation="MPSVLQQLQARGVTFVVPETTHVAPDVNPERIAPGVVIHPGCRL
                     RGTSLSIGPGCVIGAEAPATVCDCQLGANVHLAGGFFDRSTFLDGFKAGSGAHVRPGC
                     LFEEESSIAHSVGVKQTVFLPWVTAGSLINFCDALVAGGTGPKDHSEIGSSYIHFNFT
                     PHQDKATPSLIGDVPNGVLLNQPPVFLGGQGGMVGPCRIAFGTVLPAGQIWRGDMTIP
                     SRLVAKPGIRASVDCAFDPLRFNNLARILRNNLIYWGNLLALDAWYRNVRTLFLRDDP
                     FAKACHAGARQRLAEILRDRMIRLEELAQKVGQSLSRPDAATDENRRFVEAWPAWRHA
                     LDRKLVSLDTEPVPEEVRQTFSAFTTGIPYLDAVRALTAEHGQTLSAWLASFPNGLDA
                     WCEALHTSDPVPHPPGMPRHRANGDIPT"
     gene            1545..2330
                     /locus_tag="PK576_07510"
     CDS             1545..2330
                     /locus_tag="PK576_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOE61476.1"
                     /translation="MSTPLPRHTRDETFCVRAYECGVRNCVTLSSYCNFLQEIAGNHA
                     RDLGLGIQALQDAGFTWMLARLRLGIRRYAAWRETLLIRTWPAGTRGRATALRDFVCR
                     DEAGDLLLEGVSEWLYVDRKANRIVRLPPDFAALAPEGTPRVTLPPAPEPPVPEPASA
                     WSTTLTVRRSDHDFNNHVNHAHYVAWALECLPDAWTGTRQVGELDIAFKNAAIWGDTI
                     LSEAAQAGDTVYHRIRRLSDQTLLASARTVWRTHENNKGGTCP"
     gene            2327..3688
                     /locus_tag="PK576_07515"
     CDS             2327..3688
                     /locus_tag="PK576_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE61477.1"
                     /translation="MKRMMYSFILAALTAGTLWAQTLSTTPTSRGEGWEKRVARDAAL
                     AKQGGWELVFIGDSITDFWQNRGKAVWEKHFSQYKALNLGISADRTEHVLWRLDHGVL
                     DGYQPKLFVVMIGTNNTGHRKPEMESTADTVAGIKTILDKLGQKAPHSHVLLLAIFPR
                     GATAQDPCRLRNEAVNAQIQKFADNKRVFWMNINDKLLQPDGTLSKEIMPDLLHPNAN
                     GYEIWAEAIAPFVTQTLRTSVTPVSRMHESWWKNRFEQKLALVKQGGWELVFMGDSIT
                     HGWEGSGKKIWDEKFGAYKALNLGYGGDRTEHVLWRLDHGELDGYQPKLLVLMIGTNN
                     TGHRPEVREHPEDTAAGIKAILGKIAEKSPTTRVVLMPIFPRSSKPTNSARIRNDKVN
                     ALIRPYADGQRVVLLDINQDLLEPDGTLTKEMMPDFLHPKEKGYTVWANALVPLLKTT
                     FGK"
     gene            complement(4062..6320)
                     /locus_tag="PK576_07520"
     CDS             complement(4062..6320)
                     /locus_tag="PK576_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE61478.1"
                     /translation="MTPKPDTTSQNPEQHAGLPVVISGLGAVTPYGIGTNVLWQALLR
                     GDSAISAMDLFDLNGIPCTRAGVVRMPVSPAGFEDAPRATRFAAEACREALDQAGLMR
                     DAAARGDTALVTASNFADMTRGERALIPADTEGRDAALARHCAQATACEALAEGFSLG
                     GLRLPISLSCASGAAAAACAAELIAAGRAQRVLVVGFDALSRFAWSGLCSLRTMSRKR
                     VRPFDADRDGTIFTEGAAALVIERADLCAARGATPLAVLAGWATGNNGHHMTAPALRG
                     AGSFRVMRDALARAGLAPDRVDHLNLHGTGTQPNDLTETEAVYDLFGQRVASLPVTSV
                     KGTLGHMLGAAGSVELAVSVLTLSHGIIPPTGNLTRQDPACLLDVVTVPRETRVRCVL
                     SNSAGFGGCNAAAVILAPDEPFCPPAVLRPAAVVITGMGVVSALGADVVECAAALREN
                     EPALAPWSRLSPEGVPPLSPVGEAPEFDLTEYGVSRKTYLDRASELFLAACGEALHSA
                     GRETGEPADEETGILAGTAWGCLSTAERFFDDYLAKGPRFVRPFLFPHAYANTAVSLA
                     AIEWGLTGPHGNFVSRETASGIALVQAIDLIRAGQARRLVAGGVDALSLTRLAEGAPD
                     AAVPGEAAAAVMLECALDAVACGSDAVARIIGCGLATDPQSAVADALAQAGCVPVAVY
                     VNGTAARDAAGALVPDVPVIAPEALCGAVQGASTVLHLVLALLAGHDGPVLVLTAEAC
                     TSVALVVEPAKC"
     gene            complement(6517..7530)
                     /locus_tag="PK576_07525"
     CDS             complement(6517..7530)
                     /locus_tag="PK576_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HOE61479.1"
                     /translation="MALFESYARRIKQIDAVCKQYGIKDLQTARKLCQDRGFDPVEIA
                     TAIQPICFENAKWAYVLGAAIALKKGCGKAAEAAEAIGEGLQAFCIPGSVADDRKVGI
                     GHGNLAAMLLREETRCFCFLAGHESFAAAEGAIGIALKANKVRTTPLQVILNGLGKDA
                     AYIISRINGFTYCETKFDYATGKLKVTKTTKFSDGPRASVRCYGADDVREGVAIMWQE
                     KVDVSITGNSTNPTRFQHPVAGTYKKECVQKGKRYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGFLGMGNNPMVGATVAVAVSVEESFAKK
                     TKV"
     gene            complement(7550..8263)
                     /locus_tag="PK576_07530"
     CDS             complement(7550..8263)
                     /locus_tag="PK576_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61480.1"
                     /translation="MSCPYPCSHEVEQMCCVAKGAKHGPAPIPEEGKWVQVKEVKDIS
                     GLTHGVGWCAPQQGACKLTLNVKDGVIQEALIETLGCSGMTHSAAMAAEILPGKTLLE
                     AMNTDLVCDAINTAMRELFLQIVYGRSQSAFSEGGLPIGAGLEDLGKGLRSQIGTMYG
                     TKQKGVRYLEIAEGYVSRLALDKNKEVIGYEFVRLGPLMELLKKGEDPKKALKTCTGT
                     YGRFSKEMGAVQYIDPRHE"
     gene            8425..9606
                     /gene="nifS"
                     /locus_tag="PK576_07535"
     CDS             8425..9606
                     /gene="nifS"
                     /locus_tag="PK576_07535"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOE61481.1"
                     /translation="MQPIYFDNNATTRVADEVVEAMRPFFSDLYGNPSSMHLFGGQIA
                     RHVQEAREKVAAFIGAEPSEIVFTSCGTESDNMAIRGSAELLGPQAKIITSRVEHPAV
                     LGPCRRLREMGFRETELPVDGSGQVDLDALRDALKDGTPAIVSLMWANNETGVVFPME
                     EIAHIVKAAGAVLHTDAVQVAGKMPIDVRRLPVDMLSFSGHKLHAPKGIGVFYLRRGT
                     RLKTFMLGGHQENGRRGGTENVPYIVGLGKAAELAAANIEAERRTVARLRDKLEKGLL
                     ATCPDARVNGDRVNRLPNTTNISFEYIEGEALLYHLSDLGICASTGSACAAGSLEPSH
                     VIRAMGVPFTAVHGSVRFSLSRYNTEAEVDTVLEHMPRIVRKLRDLSPFGKGDTLPVE
                     L"
BASE COUNT         1975 a         3217 c         2845 g         1802 t
ORIGIN      
        1 atgtcagtcc gacggtcgtg gactttaatg acgacggcgt gccggacttc atcggcggcg
       61 cggaagacgg ttacctctac tatctgcgca acccgcgcac atctgcccgt tcccgctaaa
      121 atccttttac acatttgctc tattcggcat atactatcgt caacttttga tggggaaaga
      181 gggataagaa tgaatgccca gcgctcaagc ggagcgaacg gcagacgaat tcgaaatccc
      241 acggggtttt tacttgagta ttgaaagatg aaagttaaaa aaacgatgcc atctgtcctt
      301 caacagcttc aggcacgcgg cgttacgttc gtggttcccg aaaccaccca cgtcgcgccc
      361 gatgtcaatc ccgaacgcat cgcgccgggt gtggtcatcc atcccggttg ccgcctgcgc
      421 ggcacgtccc tttccatcgg cccaggctgc gtcatcggtg cggaagcgcc tgccacggtt
      481 tgtgactgcc aactcggtgc caacgtgcat ctggccggcg gtttttttga tcggtcgact
      541 tttctagacg gtttcaaggc gggcagcggg gcgcatgtgc ggccgggttg tttgttcgag
      601 gaagagtcgt cgatcgcgca cagcgtgggt gtcaagcaaa cggtcttcct gccgtgggtc
      661 acggctggca gtctgatcaa cttctgcgat gcgctggtgg ctggcggcac ggggcccaag
      721 gaccattcgg aaatcggctc ttcctacatc catttcaact tcaccccgca tcaggacaag
      781 gccaccccct cgctgatcgg cgacgtaccc aacggcgtgc tcctcaatca accgccggtt
      841 tttctgggcg ggcaaggcgg aatggtggga ccctgccgga tcgccttcgg caccgtgctc
      901 ccggccgggc agatctggcg tggcgacatg accataccca gccgccttgt ggccaaaccc
      961 ggaatccgcg cgagtgtgga ctgtgcattt gatccgttgc gtttcaacaa cttggcacgt
     1021 atcctccgca acaatttgat ctattgggga aaccttctgg ctcttgacgc ctggtaccgc
     1081 aatgtgcgca cgctcttcct acgcgacgac ccttttgcga aggcctgcca tgcgggtgcg
     1141 cgtcagcggc tggccgaaat cctgagggat cgcatgatcc ggttggagga actcgcccaa
     1201 aaggtcggac agagcctcag ccgtccggac gcggccacgg atgagaaccg ccgctttgtc
     1261 gaggcatggc ccgcctggcg gcacgcgctt gaccggaaac tcgtcagcct ggatacggaa
     1321 ccggtccctg aagaggtccg ccaaacgttt tccgcgttca ccaccggtat tccctatctc
     1381 gatgcggtcc gcgccctgac cgccgaacac ggacagacgc tctccgcatg gcttgcctct
     1441 ttccctaacg ggctcgatgc ctggtgcgaa gccctccaca cctccgaccc cgtaccccac
     1501 cccccgggga tgccgcggca tcgggccaac ggagacatcc cgacatgagc acccccctgc
     1561 cacggcatac ccgtgatgag acgttttgcg ttcgcgcata cgagtgcggc gtgcgcaact
     1621 gcgtgacgct gtcctcctac tgtaacttcc tgcaggagat cgcgggtaac catgcgcgcg
     1681 atttggggct cggcattcag gcgttacagg acgcgggctt cacttggatg ctggcgcgtc
     1741 tccgtctggg catccgtcgc tatgccgcct ggcgcgaaac gctgctgatc cggacttggc
     1801 ccgccggaac acgcggacgg gcgaccgccc tccgcgattt cgtgtgccgg gacgaggccg
     1861 gcgacctgct gttggagggc gtaagcgaat ggctgtatgt ggaccgtaaa gccaaccgga
     1921 tcgtgcggct gccccccgat tttgccgcgc tggccccgga aggcacaccg cgagtcacgc
     1981 tgccgcctgc gccggaaccg cccgtgccag aacccgcaag cgcatggtcc accaccctga
     2041 ccgtccgccg cagtgaccac gatttcaaca accatgtgaa tcatgcacac tatgtggcgt
     2101 gggctttgga gtgcctgccg gatgcctgga ccggcacacg tcaggtcggc gaactcgata
     2161 tcgccttcaa gaacgccgcc atctggggcg acaccatttt gtcggaagcg gcgcaggccg
     2221 gcgacaccgt gtaccaccgc atccggcgtc tgtctgacca gacccttctg gcaagcgccc
     2281 gaacggtctg gcgcacacac gagaacaaca agggaggaac atgtccatga agcgtatgat
     2341 gtattcgttc atcctggccg cgttgacggc cgggacactt tgggcccaaa ctctttcgac
     2401 cacgccaacc tcgcgtggcg aaggttggga aaaacgggtg gcgcgcgatg ccgcgctcgc
     2461 caaacagggc ggttgggaat tggtgttcat cggcgattcg atcaccgatt tctggcagaa
     2521 ccgcggcaaa gcggtttggg agaagcactt cagccaatac aaggcgctca atctgggcat
     2581 cagcgccgac cggaccgaac acgtgctctg gcgtctggac catggcgtgc tggacggcta
     2641 ccagcccaaa cttttcgtgg tgatgatcgg caccaacaac accggtcacc gcaaaccaga
     2701 gatggagagc actgcggaca cggtcgcggg tatcaagacg attctggaca aactgggcca
     2761 aaaggcgccg cactcccatg tgctgctact cgcgatcttc ccgcgcgggg ccacggccca
     2821 agacccctgc cgtctgcgca acgaggcggt gaacgcgcaa atccagaaat tcgcggacaa
     2881 caaacgcgtg ttctggatga atatcaacga caaactcctc cagcctgatg gcacgctctc
     2941 caaagaaatc atgcccgact tgctgcatcc gaatgcgaac ggttatgaga tctgggccga
     3001 ggcgatcgcg ccgttcgtga cgcagacgct ccgcacctct gtcactcccg tgagccgcat
     3061 gcacgaaagc tggtggaaaa accgttttga gcagaagctg gccttggtca aacaaggcgg
     3121 atgggaactg gtcttcatgg gtgattcgat cacgcacgga tgggaaggca gcggcaagaa
     3181 aatctgggat gagaagttcg gcgcatataa ggcactcaac ctcggctacg gtggcgaccg
     3241 caccgagcat gtgctgtggc gtctggatca tggcgaactg gacggttacc agcccaaact
     3301 cttggtgctg atgatcggca ccaacaacac tggtcaccgg ccggaggtgc gggaacatcc
     3361 ggaggacacg gccgccggca tcaaggccat ccttggaaaa atcgcagaga aatcacccac
     3421 tacccgcgtg gtgctgatgc cgatcttccc ccgcagttct aagccgacta acagcgcacg
     3481 catacgtaac gataaggtca atgcgctgat ccggccttat gcggacggcc agcgggttgt
     3541 cctgctcgac attaaccaag acctgctgga gccggacggc accctcacaa aagagatgat
     3601 gccggacttt ctgcatccca aggagaaagg gtacaccgtg tgggccaacg ctctggtgcc
     3661 gctcctgaaa acgaccttcg gaaagtgatc cgaaacggat gaacgaaacg gggcggccat
     3721 tccggcagga aaggccgcct tgtttttgta cccgcgatgc taaaaaatcg tgctctgcgg
     3781 acgcctcggg gaggcgtccc caccaacgaa tgaactttcc tggctacgac cccatcggga
     3841 gtagagtgtt gaccacaggg aacacagaga ccgcagaaca ttttgccaat gcactcaacc
     3901 agtacaagct cgtctccaat cctgtaaatc ctgttaatcc tgtcttgttc ttccccgacc
     3961 tcaacgctcc cgtcttccgc aaagcgggaa atggcgcaca ggtagggcgg tttccccgaa
     4021 accgccgcgg acgcctcggg gaggtgtccc taccgatcac ttcagcattt cgccggttcc
     4081 acgaccaagg ccacactggt acacgcctct gcggtcaaca ccaataccgg tccgtcgtgt
     4141 ccagccagca gggccagcac gagatgcagt accgtggagg ccccctgcac cgcgccgcag
     4201 agcgcctcgg gcgcgatcac cggcacatcc ggaacaagcg cacccgccgc gtcccgcgca
     4261 gcggtgccat tcacatagac agcgaccggc acacacccgg cctgtgccag cgcgtccgca
     4321 accgctgatt gcggatccgt agccaacccg cagccgatga tccgcgcgac cgcatcgctg
     4381 ccgcaggcca ccgcatccag agcacattcc agcatcaccg ccgctgccgc ttcgcccgga
     4441 accgcggcat ccggcgcacc ttccgccaga cgagtcagcg aaagcgcatc cactccgccc
     4501 gccaccaacc gtcgcgcttg tccggcacgg atcaaatcga tcgcctgaac cagcgcgatc
     4561 ccggacgcgg tttcacgcga aacgaaatta ccgtgcggtc cggtcaagcc ccactctatc
     4621 gcagccaaac tcaccgccgt gttcgcatag gcatgcggaa acaggaaggg cctcacaaaa
     4681 cgcgggcctt tggcgagata atcatcgaaa aaccgttcgg ctgtggatag acacccccaa
     4741 gcggttcccg ccagtatgcc tgtctcctcg tccgccggtt ctccggtctc ccgccccgcc
     4801 gagtgcaacg cctcgccgca ggcggccaaa aagagttcgc tcgcacggtc cagataggtc
     4861 ttccgcgaca cgccgtactc ggtcaaatcg aactccggtg cctcaccgac cggggacagc
     4921 ggcggcaccc cttctggcga aaggcgggac cacggggcga gggccggttc gttttcacgc
     4981 aacgcggccg cgcactcgac gacatccgcg cccaacgcac tgaccacgcc catcccggtg
     5041 atcacgaccg cagcgggccg caacaccgca ggcgggcaaa aaggctcatc cggcgcgagg
     5101 atcactgccg ctgcattgca tccaccgaat ccggcggagt ttgaaagcac gcatcgcaca
     5161 cgcgtctcgc gtggaaccgt caccacatcg agcagacacg ccggatcctg ccgcgtcagg
     5221 ttgccggttg gcgggatgat cccatgactg agcgtcaata ccgacaccgc gagttccacc
     5281 gatccggccg cgccgagcat atggccgagc gtccccttta ccgaggtcac cggtaacgag
     5341 gccacccgct ggccgaagag atcgtacacc gcctccgttt ccgtcaagtc attgggttgg
     5401 gtaccggtgc cgtgcagatt gagatggtct acccggtcgg gagcgagacc cgcgcgcgcg
     5461 agcgcgtcac gcatcacccg gaacgaaccg gccccgcgaa gggcaggcgc agtcatgtgg
     5521 tggccattgt tccccgtggc ccaaccggcc agcaccgcga gcggcgtggc accgcgcgcc
     5581 gcgcagagat ccgcgcgctc gatcaccagc gcggcagccc cttcagtgaa gatcgtgccg
     5641 tcacggtcag cgtcaaaggg cctcaccctc ttccggctca tggtccgcaa cgaacagagg
     5701 ccactccacg cgaagcgcga gagggcgtca aaccccacca ccagtacacg ttgcgcgcgt
     5761 ccggccgcga ttagttccgc cgcacaggcg gcggcggcgg caccggaagc acatgagagc
     5821 gaaatcggca ggcgcagccc gccgagtgaa aaaccttcgg ccagcgcctc gcaagcggtg
     5881 gcttgcgcac aatgacgcgc cagagccgca tcgcggcctt ccgtgtccgc agggatcagt
     5941 gcccgctcgc cacgcgtcat gtccgcgaag ttggatgctg tgacaagcgc ggtgtcgccg
     6001 cgtgccgccg catcccgcat cagccccgcc tgatccaatg cctcacggca ggcctcggcg
     6061 gcgaaccgcg tggcacgcgg cgcgtcctcg aaccccgcag gcgaaaccgg cattcgaacc
     6121 acccccgcac gcgtacacgg gatgccgttc agatcaaaaa gatccatcgc ggagatcgcg
     6181 ctgtcgccgc gcaacagcgc ctgccacaac acgttcgtcc cgatgccgta gggggtgact
     6241 gcccccaaac cggagatcac aaccggaaga ccggcatgct gttcggggtt ttgcgaagtc
     6301 gtgtcaggct tgggtgtcat cgggctcctt ctcttctttt ccgggcacag gagagcaagt
     6361 gcaatatcgg accgcagggg tgtctcacgg aagattaagt atatacctta tcgggccgtt
     6421 gcggcaactg gttcacccaa ttggaattga tgggcaaaaa acgagccgcg ccgcaactgc
     6481 ggcacggcct gtttctttgg atcacgcttt cgggcgctac accttcgttt tcttggcgaa
     6541 gctctcctcc acagagaccg cgaccgcaac agtcgcgccg accatcgggt tgttacccat
     6601 gccgaggaaa cccatcattt cgacgtgcgc cggcacggag gacgatcccg cgaactgcgc
     6661 atcggagtgc atccggccca tggtgtcggt catgccgtac gaagccggcc ccgccgccat
     6721 gttgtcggga tgcagcgtac gccccgtgcc tccgcccgaa gccacggaga aatagcgctt
     6781 gcccttctgc acgcactctt tcttgtaggt gcccgcaacc ggatgctgga agcgcgtggg
     6841 gttggtggaa ttaccggtga tggaaacatc caccttctct tgccacatga tcgccacacc
     6901 ctcgcgcaca tcgtccgcac cgtaacaccg taccgacgcg cgggggccgt ccgagaactt
     6961 cgtcgtcttc gtgaccttca gtttgccggt cgcgtagtcg aatttcgtct cgcagtaggt
     7021 gaagccgttg atgcggctga tgatgtaggc ggcgtcctta cccaatccgt tgagaattac
     7081 ctgcagcggc gtggtgcgga ccttattcgc cttgagcgcg atgccgatcg ccccttcggc
     7141 cgccgcgaat gactcatgcc ccgcgagaaa gcagaaacag cgcgtctcct cacgcagcag
     7201 cattgccgcc agattgccgt gtccgatccc gaccttacgg tcatccgcca cggaaccggg
     7261 aatacagaag gcctgcaagc cctcgccgat cgcttccgcc gcttcggcgg ccttgccaca
     7321 tccctttttc aacgcgatgg ccgcgcccag cacatacgcc cacttcgcgt tttcgaagca
     7381 gatcggctga atcgcggtgg cgatctcaac cggatcgaac cctcgatcct gacacagctt
     7441 ccgggccgtc tggagatcct tgatcccgta ctgcttgcac acggcgtcga tctgcttgat
     7501 gcgccgcgca taactttcaa aaagtgccat aagaatagtc tcctttcacc tactcgtgcc
     7561 gcggatcgat gtactggacg gcccccatct ccttggagaa acggccgtac gttccggtgc
     7621 acgttttcaa cgcctttttg ggatcctcgc cctttttgag cagctccatc aacggcccca
     7681 ggcggacgaa ctcgtacccg atgacttcct tgttcttgtc gagcgccagc cgactgacat
     7741 atccttcggc tatttccaga taccgcaccc ccttctgctt ggtgccgtac atcgtgccga
     7801 tttggctgcg gagccccttg cctaaatcct cgagacccgc accgatcggc aggcccccct
     7861 cggaaaacgc gctctgactg cgtccgtaga cgatttgcag gaagagttcg cgcatggccg
     7921 tgttgatggc atcgcacacg agatccgtgt tcatcgcctc cagcaacgtt ttgcccggca
     7981 ggatctcggc cgccatcgcg gctgagtggg tcatccccga acagccgagc gtctcgatca
     8041 gcgcctcctg aatcacgccg tctttgacat tcagcgtcaa cttgcaggcc ccctgctgcg
     8101 gtgcgcacca gcccacaccg tgcgtcagac cggaaatatc cttgacctct ttcacctgta
     8161 cccatttccc ctcctcgggg atcggggccg gtccatgctt agcgcctttg gcgacgcagc
     8221 acatctgctc cacttcatgg gagcacggat acggacaact catcgtgttc gacctccatt
     8281 gctgttctaa gaaaccgcgt gatgataccg gaaacgtaaa ccgaacacaa cggaaatgct
     8341 tctgattcgc cacttgagtt ccttcctgca gtggtgtaac gttggggcac cttgttttac
     8401 ggagcgggat acaggaacaa ctcgatgcaa cccatctatt tcgacaacaa cgccaccacc
     8461 cgcgtcgcgg atgaagtggt ggaagcgatg aggccgttct tcagcgacct gtacggcaac
     8521 ccttccagca tgcatctctt tggcggacag atcgcccggc atgttcagga ggcgcgcgaa
     8581 aaggtggcgg cctttatcgg agccgaacct tcggagatcg tattcaccag ttgcggaacg
     8641 gagagcgaca acatggcgat ccgcggaagc gcggagttgc tgggaccgca ggcgaaaatc
     8701 atcacctcac gggtggaaca ccctgcggta ctcggaccct gccgccgttt gcgggagatg
     8761 gggttccgtg aaaccgaact gccggttgac ggcagcggac aggtggattt ggatgcgtta
     8821 cgcgacgcgc ttaaggacgg gacccccgcc atcgtgagcc tcatgtgggc caacaacgaa
     8881 accggagtgg tcttcccgat ggaagagatc gcccatattg tcaaagccgc cggagccgtg
     8941 ttgcacacag acgccgtaca ggtcgcgggc aagatgccga tcgatgtgcg gcggcttccg
     9001 gtggacatgc tctcgttctc cggacacaag ttgcacgcgc ccaaagggat cggcgtgttt
     9061 tacctgcggc gcggcacacg cctcaagacc ttcatgctcg gcggccatca ggaaaacgga
     9121 cgacggggcg gcacggaaaa tgtcccttac atcgtgggtc tggggaaagc ggccgaactg
     9181 gcagccgcca acatagaggc ggagagacgc accgtggccc ggctacgcga caaactggag
     9241 aagggcctgc tcgccacctg cccggacgcg cgtgtcaacg gcgaccgcgt gaaccgtctg
     9301 cccaacacca ccaacatcag cttcgaatat atcgagggcg aggcgctcct ctatcacctc
     9361 agtgatctcg gaatctgcgc ctcgaccggt tcggcttgcg cggccggctc gctcgaaccg
     9421 tcgcatgtga tccgcgcgat gggtgtgccg ttcacggcgg tccacggctc ggtgcgcttt
     9481 tcactcagcc gctacaacac ggaagcggaa gtggacaccg tacttgaaca catgccgcgc
     9541 atcgtgcgca aactgcgcga cctctcgccc ttcggcaaag gcgataccct gccggtggag
     9601 ttgtagaacg tcgaacgcag aaaacgagga gtgaaaaaac ttcgacgttg ggcgttctga
     9661 gttcggcgtt ctgagttcaa atttttttca acacgacctt gatctccacg gcttcgtgca
     9721 tgttttccgc cgggatgaaa aaggatttca ccaaccgttc gggcaaccat gcctgccgcg
     9781 cttcttcaag gtcgtcggat gctttgatcg tggagtcggc agcgaccccc gccaaaacc
//
LOCUS       DAOSLE010000099         9827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6668_length_9827_cov_8.732603, whole genome shotgun sequence.
ACCESSION   DAOSLE010000099 DAOSLE010000000
VERSION     DAOSLE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6668_length_9827_cov_8.732603"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..3067)
                     /locus_tag="PK576_07540"
     CDS             complement(236..3067)
                     /locus_tag="PK576_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE61482.1"
                     /translation="MLIRLVFKTLRRGKMRFAMATLGVASATGLMVWSLGLTVTAMGQ
                     SRETVRRMTAPFSCWVAAEGAGYRPARGAARPSVPPAALPPQLMESVLSRPEVESAFA
                     CRVIHATLDFRPGGAVLQGPPLVTSLTLAPQEGCPYTEAEVTGDWPDPSSPEPVVAIC
                     SSVFARRHLPPPPCGTPLVLITPHSTVTVRIGAVIDFPQAVDGLPTAFATIGALRQAA
                     GGVWDPRPNLLLCTLRTGAAPDDLRAATGLPPPSTTTGSAYGGTRPGPNAPARFILTD
                     RRQIEARHVSDNLANFKRQAPLLLTLSVLTAFCLLFNALTLGVAQNVRGIALLRAAGM
                     TVGHVAQMVALEGFIMACCGWTLGLLGGWAALTLFTRRAAQTFPAGVMLGWITPAVTA
                     AGIMVLTLLSLLWPCRRAMRIAPLEALAEHDAASQPDRDTRKRSFLRTACGFALLFPM
                     LVFVLPLRLTALSRSVLLLTVGIPLHLFGLFLLLPGFIRATERLCGPVVSALLALDPV
                     LLHRRISRDIPRTAGMTITLAVGLGSFAAIHIWGSSLTRPFLPSPTFPDVIVSLLPNG
                     VSRSAAGAVALLEGVGQQRCLAIEAVQVPLAPALTTRVARVSGRPSPHPNVLLFGADP
                     RTAFGGDRPLAPFRFVEGEPRQAADALTRNDACLITLMFARETGLRKGGTLQLTKRPL
                     PARARSAPSGSRSEEMVSFTVVGVVDLNWHLVTSRAQMRGRHGMPGGTMGPVFVNEEV
                     ARQWSGNTETTCFLWLNFSEAYRAMDPLAAGQRLEADIRKTLAVGSGNTVRVHHRDEI
                     ADGTIAHSADLIGDMARAPFWSLIVLSTGMATLLIASFHASAREIAVLRAIGMTRGQL
                     GRLLLAEALMTSVCGIVLSLVSGLCIGWTFTGWTRAWMPFGGLPVTLEIPWPTLFRGI
                     GFAFALCALMALPPILWLVRRPRFARECN"
     gene            complement(3222..3968)
                     /locus_tag="PK576_07545"
     CDS             complement(3222..3968)
                     /locus_tag="PK576_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61483.1"
                     /translation="MTAEEGMALEVRKLGKSFGSGANRVTVLDGVNLNLRRGAFDAVM
                     GPSGSGKSTLLHLLAGLLTPDAGEIRVGGTCLSGMPDSAATCFRRRRIGLIFQDFNLI
                     PTLTAEENILLPLLLDRRAPHTGELAALLERLGLTALRHRVPAKLSGGERQRVAIARA
                     LVTTPDLVLADEPTGNLDSPAGRAFCDLLRDIHTERGCTILLVSHDPVVAAAAERVHL
                     LKDGRVVGELETQHDPARISKRYVEILRQP"
     gene            complement(5079..5756)
                     /locus_tag="PK576_07550"
     CDS             complement(5079..5756)
                     /locus_tag="PK576_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOE61484.1"
                     /translation="MSFFTNLTRGIIRENPTFRLVLGMCPTLAVTTSLENALGMGLAA
                     TFVLVCSNMVISSLRKLIPAAVRIPCYIVIIAAFVTMVDLLMQAYTYALWEKLGIFIP
                     LIVVNCIILGRAEAFASKNGVLNSAADGIGMGIGFTIALSVIATLREFIGNGSVTVWG
                     DLAFTAIHNRAMVLFILPAGGFITLGLILAFLNHLQAVSAQRRGGAAPLPLELDCRHC
                     ALCKPVE"
     gene            complement(5876..6781)
                     /gene="cysK"
                     /locus_tag="PK576_07555"
     CDS             complement(5876..6781)
                     /gene="cysK"
                     /locus_tag="PK576_07555"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE61485.1"
                     /translation="MAGWTSILGTIGNTPLLKLDRIASFPLYAKAEYLNPAGSVKDRV
                     ALHMIEQAERRGDLRPGMTLAEATSGNTGIGIAFVGVRKGYRVIIVMPDNMSEERRKI
                     IRAFGGELVCTPAAESLNGCLRKLEELRQEIPGLFILGQFVNPDNPEIHYLTTAREIW
                     EQMEGRVDAFVAGVGSGGTLSGVGRFLKERDPAIRVVAVEPAQASALLGQEPGLHRIE
                     GIGDGFIPDVLDVSIIDDVITVTDEQAITTAQQLTRVEGALVGTSAGANVWAAIETGA
                     RIGHDRRIVTILPDRAERYFSTALI"
     gene            complement(6952..8373)
                     /locus_tag="PK576_07560"
     CDS             complement(6952..8373)
                     /locus_tag="PK576_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE61486.1"
                     /translation="MRALLTVLLLTAGQGCLCPAVKPLPSGLACRGLFHPAPDALFLR
                     DRTGLDTAGDRVVEQQIFDCALALIRDAEQLVVADLFLFQPFDGTGPAPHRPLCEELT
                     AALIRRKREVPSLRAILITDPVNTVYGGRNAPHLCRLRNAGVEVVITDLTRLRDSNPL
                     WSGLWHPLIRPWGNRADAGWMPNPFGAGRVTLRSWLALLNFKANHRKVVIADSGGELR
                     ALVTSANPHDSSSAHTNVGLLFSGPAAHDALACEAAVLAFSGGAAAPEFNSGGKPAVV
                     ESPAWVAVVTEDAVAEAAEELIGGAGRGDMLDLVMFYLADRRIVHALLAAARRGVTVR
                     VLLDPNRDAFGRQKNGMPNRQTARELVRAGVQVRWAETRGEQMHAKLLLRRSVSDTAD
                     LLLGSANFTRRNLRGYNLETDAWLSGSPDAPAFAAATAFVETLWENRGGRRFSTGYDA
                     FRDDSRLRVILARIQEATGLCTW"
     gene            complement(8771..>9827)
                     /locus_tag="PK576_07565"
     CDS             complement(8771..>9827)
                     /locus_tag="PK576_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61487.1"
                     /translation="IDCCAHAVARSDKCNDGGDSDPDPDGDSDGAAMEPRNALSSHTP
                     VTNRLPSPVDHVLTRSSRKCKVFDIETEVRMRTLVLGAMVCLLSGFAFTQDLLSELAG
                     PAEAYKTAVEALEKQRLSDMAQAARTYVSVLDGIEKSATAKGEVKIVEAAARERKAAL
                     AGRLAAELPAELPALRLQGSRRALLMKQEKVNVDAAGRKKRLDSQYLGVLASLAAKAE
                     PGSTLGKQVAAEREAVLAAGKEGAGGGKASGGEAKASQVPRGKNVVVNGDFEKVDALG
                     KPEGWLFHGPVSVEKEERNTFVRIKGGTVAGGYYRQEMERPNNVTHGTIRAKLRTHGR
                     IGLDRSRGSANISCKNK"
BASE COUNT         1973 a         3104 c         3060 g         1690 t
ORIGIN      
        1 tttgttgtga cgtttcatgt gattcatggt gcggattgtc tggtgcctac ggggggcatc
       61 cggggatcgc gcgttcgttc cggacttgcc tataaattgc cgggatgaca ggattttcag
      121 gattgaacaa catcgtgtta atcatgtcct aaacttattc caaggttttt cgcggctgag
      181 ccgcgcggca ggctaaatcc tccctccttg cctatacctt acatcaaagt tatcctcagt
      241 tacattcccg tgcgaagcgc gggcggcgga ctaaccagag gatcggcggc agcgccatca
      301 acgcgcagag cgcgaaggcg aatccgatgc cgcggaacag ggtcggccaa gggatctcca
      361 gcgtgaccgg caggccgccg aacggcatcc aggcccgggt ccagccggtg aaggtccagc
      421 cgatgcagag tcccgagacc agactcagca caatgccgca cacgctggtc atcaacgctt
      481 cggccagcag cagacggccc agctgaccgc gcgtcatgcc gatcgcgcgc agaaccgcga
      541 tctcgcgtgc ggaggcgtgg aaggaggcga tcagcagggt ggccatgccg gtcgatagca
      601 cgatcagcga ccagaacggc gcacgggcca tgtccccgat aaggtcggcg ctgtgcgcga
      661 tcgtgccgtc ggcgatctcg tcgcggtgat gcacgcgcac ggtgttgccg ctgccaaccg
      721 cgagggtctt acggatgtcg gcctccagcc gttgtcctgc ggccaacgga tccatggcgc
      781 ggtacgcctc gctgaagttc agccagagaa aacacgtggt ttccgtgttg ccgctccatt
      841 gccgcgcaac ctcctcgttc acgaaaaccg gccccatcgt accgcccggc atgccgtgac
      901 ggccacgcat ctgggcgcgg gaggtcacca gatgccaatt gaggtccacc acgccaacga
      961 cggtgaacga aaccatttct tcggaccgcg aaccggacgg ggcgctacgg gcgcgggccg
     1021 gaagcgggcg tttcgtgagc tgcagggtcc cgcctttccg caaccccgtt tcgcgcgcga
     1081 acatcagggt gatgagacaa gcatcgttcc gggtcagggc gtcggcggcc tgacgcggtt
     1141 cgccttccac gaagcggaac ggcgccagcg ggcggtcgcc gccgaaagcg gtccggggat
     1201 ccgcaccgaa cagcagcaca ttcggatggg gtgaaggtct tcccgagacg cgcgccacac
     1261 gcgtggtgag tgccggagcc agcggaacct gaaccgcctc gatggcgagg caacgttgtt
     1321 ggccgacgcc ttccagaaga gccacggcgc ccgcggcact gcggctgacg ccgttcggca
     1381 gcaacgacac gatcacgtca ggaaacgtgg ggctgggaag aaagggacgg gtcagcgaac
     1441 tgccccagat atgaatcgcg gcaaaggagc ctaacccgac cgccagggtg atcgtcatgc
     1501 ctgccgtgcg cgggatatca cggctgatcc ggcggtgcaa caggaccgga tcgagcgcca
     1561 gcaacgcgct gacaaccggt ccgcacaggc gttccgtggc gcggatgaag cccggcagaa
     1621 gcaggaacag accgaaaagg tgcaacggga tcccgacagt caacagcagc acactccgcg
     1681 aaagggctgt cagccgcagg ggcaggacga aaaccagcat ggggaaaagt aacgcgaacc
     1741 cgcacgccgt gcgcaagaag gagcgtttgc gcgtgtcgcg gtccggctgg ctggcggcat
     1801 cgtgttcggc caacgcttcc agcggcgcga tacgcatcgc gcgacggcag ggccagagca
     1861 ggctcagtaa cgtcagcacc atgattcccg cggccgtgac cgcaggggta atccatccca
     1921 gcatgacccc tgcgggaaag gtctgtgcag cccgccgcgt gaaaagcgtc agcgcggccc
     1981 atccgcccag tagcccgagc gtccaaccgc agcaggccat gatgaaccct tccagcgcca
     2041 ccatctgcgc aacgtgcccg accgtcatgc ccgccgcgcg cagaagggcg atccccctca
     2101 cgttctgcgc cacgccgaga gtcagggcgt tgaagagcag gcagaacgcg gtcaacacgg
     2161 aaagcgtcag aagcaacgga gcctgacgct tgaagttcgc gagattgtcg ctgacgtgcc
     2221 gcgcctcgat ctgccgtcgg tccgtgagga tgaagcgggc aggcgcgttt ggtccggggc
     2281 gggtcccgcc gtatgccgat cccgtagtgg tggagggtgg cggcagaccg gtggcggccc
     2341 gcaagtcgtc gggcgcggca cccgtgcgca gggtgcagag cagcaggttc ggccgcggat
     2401 cccagacgcc gcccgcggcc tggcgcaggg cgccgatggt ggcgaatgca gtcggcaacc
     2461 cgtcaaccgc ttgggggaaa tcgatcaccg cgccgatccg gaccgtcacc gtgctgtggg
     2521 gggtgatgag gaccagcggt gtgccacacg gtggcggcgg aagatgacga cgggcaaaca
     2581 ctgatgagca gatggccacc accggttccg gactggacgg gtccggccag tcgccggtga
     2641 cctccgcctc ggtgtagggg cagccttcct gcggcgcgag cgtgagcgaa gtcacgagtg
     2701 ggggaccttg cagcaccgcg ccgccgggtc ggaaatcgag ggtggcgtgg atcacccggc
     2761 aagcaaatgc ggattcgacc tccggccgtg acaggacgga ttccatgagt tgggggggca
     2821 gcgcggccgg cggaacggac ggccgcgccg cgccgcgcgc cgggcggtat cccgcgccct
     2881 cggccgcgac ccagcaggag aaaggcgcag tcatgcggcg gaccgtttca cggctctgac
     2941 ccatggcggt gacggtcagg cccagtgacc aaaccatcag gccggtggcg gaggccacac
     3001 cgagggttgc catggcgaaa cgcatcttgc cgcgtctcaa cgttttgaat accaatcgaa
     3061 tcagcatggt ttttaccttc cgtaccggct tcacgggcca gcagacgctt gctccgcctg
     3121 tcagacctta cgttgacccg cgactagcgg cctgcggtgt cggaagcgcg gacgtcctcg
     3181 cccgctggca gggcggtttt ccagaaaccg ccgcagacac atcagggctg acgcaggatc
     3241 tccacatacc gtttggagat ccgggccggg tcatgctggg tctccaactc gccgaccaca
     3301 cgtccgtcct tcagcaggtg cacccgttcc gcagcggcgg cgaccacggg atcatgactc
     3361 accagcagga tcgtgcatcc gcgttctgtg tggatgtcgc gcagcagatc gcaaaaggcc
     3421 cggccggccg gggaatcgag attgccggtc ggctcgtccg ccagcacgag atcaggggtg
     3481 gtcaccaacg cacgcgcgat ggccacacgc tgccgttcgc cgccgctgag tttcgcgggg
     3541 acgcggtggc gcagggcggt cagtccgagc cgttccagca gggcggcaag ttctccggtg
     3601 tgcggggccc ggcggtcaag cagcaacggc aggaggatgt tctcctctgc cgtcagggta
     3661 gggatcaggt tgaagtcctg aaaaatcagt ccgatacggc ggcggcggaa acaggtggcc
     3721 gcagagtccg gcatgccgga aagacaggtg ccgcccacac ggatttctcc ggcgtccggg
     3781 gtgagcaatc ccgccagcag gtgcagcagg gtactcttgc ccgagccgct tggtcccatc
     3841 acggcgtcga acgcgccgcg ccgcaagttc agattcacgc cgtccagcac ggtcacgcgg
     3901 ttcgcgcccg aaccgaaact cttgcccagt ttccggacct ccaatgccat gccttcctct
     3961 gccgtcatgc tcctctcctg ttggtacgga cgggaaccca cagggactgc cgggtggtgc
     4021 cgcgccgccc gccgtacgcc ttctttttgt tcaacggcca cagacggctt tcggttcatt
     4081 ttggggccgg tttacgcgcc gggaaaaaaa attaaaaaaa gggggttgac actttgtcgg
     4141 ctttgatgta tcctcacggc aaagatcggg atgatagtaa tcatgatcgg tctgtggggc
     4201 ataaggagaa aataaggaga aaggaggcgg atttagagtt tccctttttg cggatgccgg
     4261 aaaacacctg ccagaatcgc gttcgggttt ttgcgggaca agatctgcgg gtgcggaaag
     4321 cgacttgatt gggtgataga cccgcacaga cgacgaggga cttgccaccc cgaggaaacg
     4381 gaccgggcaa caagtatggc ggcttacgcc aataaacaca gcggttgcaa cgcggttgag
     4441 agtacgaagg agaggcaatg catccatcat cgctgattgg aaaaaaatcg ggattctttc
     4501 caaggtgcgt ttgaaaaggg gaaagaggca aacataaccc ccggaacgcc tgaagggcga
     4561 accggagtcg attcaagaca atacgaaagg aagtgttttc tgactaccga aacaataagc
     4621 gtagacgctt cataagggtt ccccgcgaaa tcgtgaggga accctttctt tttaggttaa
     4681 gcgtatccga cgaagtctgt gtccctgcgc acagcgcggg gcacggcggc gtgaccgccg
     4741 cggacgagca gacgctcgtc cctcccctct tgcgggcaaa gacaaaaaag aaaaatctgc
     4801 gcaatctgtg caaagtcgac ccgcgacgag cgacctgcgg tgtcggaaac gcgggcgtcc
     4861 ctgtcgatcg tcgcgggtcg aatgtcgcgg gtcgcgggtc gacgataccg ataccgatag
     4921 cgataccgac cccgatttgg atgaaaccag aaaccatgaa accctgaaac cagaaaccct
     4981 gaaaccatga aaccctgaaa ccttgaaacc aaagagagtt gagccttcaa ctgctctttt
     5041 taagttaaaa accggatcgc aactttgagc cgagccgatc actcgacagg cttgcacagg
     5101 gcgcagtggc ggcagtcgag ttcgagaggc agcggggcgg caccgccgcg gcgctgcgcg
     5161 gagacagcct gcaggtggtt gaggaaggcc agaatcaggc cgagggtaat aaacccgccc
     5221 gcaggcagaa tgaaaagcac catcgcacgg ttgtggatcg cggtgaaggc gaggtcaccc
     5281 cagacggtga cgctcccgtt cccgatgaat tcacgcagcg tggcgatcac ggagagcgcg
     5341 atcgtgaacc cgatccccat gccgattcca tccgccgcgc tattcagtac gccgttcttg
     5401 gatgcgaagg cttcggcccg tccaagaatg atgcagttca ccacgatgag cggaatgaaa
     5461 atcccgagct tctcccacag ggcgtaggtg taagcctgca tcagcaagtc caccatggtg
     5521 acgaacgccg cgatgatcac aatgtagcag gggatgcgca ctgcggcggg aatgagtttg
     5581 cgcagcgagg agatcaccat attggagcag accagcacga acgtggcggc caaacccata
     5641 ccgagggcat tttccagcga cgtggtcacg gccagcgtgg gacacatccc gagcaccagc
     5701 cggaaggtcg gattctctct gatgatgccg cgggtcaggt tagtgaaaaa gctcatgatt
     5761 ccccagaggt tgcaaacgga aaatccatag tatccggaaa gacggcgaaa aaagcaaggc
     5821 ccgccgaacc agaatgttca gcggatacaa ctccccgcac tgacccgatc cgacctcaga
     5881 tcagtgcggt gctgaaataa cgctcggcgc ggtcaggcag gatcgtgacg atccttcggt
     5941 catgcccgat gcgcgccccg gtttcgattg cggcccagac attcgccccg gctgaagtac
     6001 cgaccagcgc cccttccacg cgtgtcagct gctgcgcggt ggtgattgcc tgctcgtcag
     6061 tgaccgtgat gacgtcatca ataatcgaga cgtccagaac gtcggggatg aacccgtcgc
     6121 cgatcccttc gatccggtgc aggccgggtt cctgtccgag cagggcggaa gcctgggcgg
     6181 gctccaccgc gaccacacgg atcgccgggt cgcgctcttt gagaaaccgg cccacgccgg
     6241 acaacgtgcc gccgcttccg acacccgcga caaacgcgtc gacccgcccc tccatctgct
     6301 cccagatttc ccgcgccgtg gtgagatagt ggatctcggg gttgtcgggg ttcacgaact
     6361 ggccaaggat aaagagaccg gggatctctt gccgcagttc ctcgagcttg cgcagacagc
     6421 cgttgaggct ctccgccgcc ggagtgcaga ccagttcgcc gccaaaggcc cggatgatct
     6481 tgcgccgctc ttcgctcatg ttgtcgggca tcacaatgat cacccgatat cccttacgca
     6541 cccccacaaa ggcgatgccg atcccagtat tcccagaggt ggcctcggca agggtcatgc
     6601 cgggacgcag gtcgccgcgc cgctccgcct gctcgatcat gtgcagcgcc acccggtctt
     6661 taacgctgcc cgccggattg aggtactcgg ctttcgcata gagggggaaa gacgcgatcc
     6721 ggtcgagctt cagtaacggt gtgttgccga tggttcccaa aatgcttgtc cagcctgcca
     6781 tgatacgaac actcctcctg ctctgcccgc gcgttccctg ccgggcgggc cagttgattg
     6841 cgaaactcta tcacacgtcc gatggcgacg caaccgcccc ggcgggagac cggcgtaacc
     6901 ggttggccgc acggggcgtg cgtgtggagc gcgtgtttcc cgctgtttcg gtcaccatgt
     6961 gcacaggccg gtggcctcct gaatgcgcgc gagaatcacc cgcagccggg agtcatcgcg
     7021 gaaggcgtcg taaccggtgg agaaacgccg accgccacgg ttctcccaca gggtttccac
     7081 aaaagcggtg gccgcggcga acgcgggggc gtccggcgag ccagagagcc acgcatcggt
     7141 ctccagattg tacccgcgca gattgcggcg ggtaaaattg gcggaaccga gcagcagatc
     7201 ggccgtgtcg ctgacactcc gccgcagcaa taatttggcg tgcatctgtt cgccgcgggt
     7261 ctcggcccag cgcacctgca cgccggctcg caccaactcg cgcgcggtct gacggttggg
     7321 catgccgttc ttctgacgtc cgaacgcatc gcggttggga tccagcaaca cgcggaccgt
     7381 gacaccgcgc cgcgccgccg caaggagcgc gtggacgata cggcggtcgg cgagataaaa
     7441 catcaccaga tccagcatgt ccccacgccc cgcgccgccg atcagctcct cggcggcctc
     7501 ggcgaccgcg tcttcggtca ctacggcgac ccatgccggg gattcgacga ccgccggttt
     7561 gccgccgctg ttgaattcag gtgccgccgc gccgccgctg aacgccagaa ccgccgcctc
     7621 gcacgcgagc gcgtcgtggg ccgcgggacc gctgaaaagc agcccgacgt tggtatgggc
     7681 gctgctgctg tcatgcgggt tggctgaggt gaccagcgca cgcaactcgc cgccggagtc
     7741 ggcgatcacc accttccggt ggttggcctt gaagttgagc agggccagcc agctccgcag
     7801 ggtgacccgt cccgcgccga acggattggg catccatccc gcatcggccc ggtttcccca
     7861 cggccggatc agcggatgcc agagccccga ccacaatggg ttgctgtcgc gcaatcgggt
     7921 gagatcggtt atcaccactt cgaccccggc attgcgaagg cggcacaggt gcggggcgtt
     7981 tcgaccgccg tagacggtgt tgaccggatc ggtgatgaga atcgcccgca gggaagggac
     8041 ctcgcgcttg cggcggatca gggctgcggt cagttcttcg catagtgggc ggtgaggcgc
     8101 cggaccggtt ccgtcaaacg gctggaaaag gaacaggtcc gccaccacga gctgttccgc
     8161 gtcgcggatc agggctaaag cgcagtcgaa aatctgctgt tcgaccaccc ggtcacctgc
     8221 ggtgtcaagt ccggtgcggt cacgaagaaa gagcgcgtcc ggcgcggggt ggaacaggcc
     8281 gcggcaagcc aaccccgacg gcaggggttt cacggcaggg cacagacagc cttgaccggc
     8341 agtcagcaga agaaccgtca acagagcgcg cacaaaacag gaacgttccg gcatgatgga
     8401 gacagcataa catgcccacg acgggaagcg ccagtcttcc ggcaatttta catttgacgg
     8461 aaaatgcgtt cgtttgtgtc taacacggac ggagcccaca acccgagcaa accgcagaaa
     8521 gtcgaagtcg atcccgcctc tttgtacgcg ctcttccctt acttgggcgt tgcgcgttcc
     8581 ttccgaagcc gagattgcgc tccggcctac ttgaacgtca cttccacgtc gtcgaagtcg
     8641 acctggccct tgcttccttt ataactgagt tcaaaacaaa accaagtcgc ctctttagga
     8701 attgcacctt ccccgtgaat cgttgtccat acgccgtctt tccgacccga tatagctatg
     8761 atccaaaaga ttacttattt ttacatgaga tattcgcact ccctctggat cgatctaaac
     8821 caatgcgccc atgcgttctc aatttcgcac ggatcgtacc atgggttaca ttattggggc
     8881 gttccatttc ttgacgataa taccctcctg ctaccgtccc tcccttaatc ctgacgaagg
     8941 tgttccgctc ctccttctcg accgaaacgg gtccatgaaa aagccaaccc tccggcttgc
     9001 cgagcgcatc gaccttctcg aaatccccgt tcaccaccac gttcttgccg cgcggaacct
     9061 gggacgcttt cgcttcacca ccgctggcct tgccgccgcc agcaccctct ttgccagccg
     9121 ccagcaccgc ctcccgctca gccgcaacct gcttgccgag cgtggacccg ggttcggcct
     9181 tggccgcgag agaggcaagc acgcccagat actggctgtc gagccgtttt ttgcgtccgg
     9241 ccgcatcgac gttcaccttt tcctgtttca tcagcaatgc cctgcggcta ccttgaagac
     9301 gcagggccgg aagttcggcg ggcaactccg ccgccagtct cccggccagt gccgcctttc
     9361 gttccctcgc ggcggcctcg acaatcttga cctccccctt ggccgtggcc gacttctcga
     9421 tcccgtccag caccgacaca taagtcctcg ccgcctgcgc catatccgac agccgctgct
     9481 tttccagcgc ttccaccgcc gtcttgtagg cttccgctgg cccagccagt tccgacagaa
     9541 gatcctgcgt gaacgcgaac cctgaaagca gacaaaccat cgctcccaaa accaacgtcc
     9601 tcatgcgcac ttccgtttcg atatcgaaaa ctttacactt acggctcgac cgcgtcaaca
     9661 cgtgatcgac tggactgggt aatcggttcg tgacaggggt gtgcgaagac aatgcgtttc
     9721 tgggttccat tgcggcgcca tcgctatcgc catcgggatc gggatcggaa tcgccaccat
     9781 cattacactt gtcgctgcgg gccactgcgt gggcgcaaca gtcgatt
//
LOCUS       DAOSLE010000100         9741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6740_length_9741_cov_7.477700, whole genome shotgun sequence.
ACCESSION   DAOSLE010000100 DAOSLE010000000
VERSION     DAOSLE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6740_length_9741_cov_7.477700"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PK576_07570"
     CDS             <1..1007
                     /locus_tag="PK576_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61488.1"
                     /translation="RAVVKYFGPGKNLTDKVKVQAFHNAAFSGMPVAEYTLAGGNLAA
                     VTNTANVVTNAIICGLAPSAEAGDYYLRAYIDSNGNNVRDVWESWGYANFYGVSDKPF
                     DPRPVTVQFVPLAESPTVTILIEDADTDQDWFPDIYEYETNPGVGFLSKSGPSTGSDG
                     DPDFEINPNLGDTTGINTLFSALMLGSTDADGDEVGDLAELILGSDGQKVSTSGDGVS
                     DGLKIGLGLSPADTLALEMTGLELTGSEATLTWKLVVDQAEGVNRNLIKSLAGAPGPA
                     TYEVLFKPSLDAADWQVVATGTATLSESEEGVSAAISRVQQALQDAPTQGFFRVRLVE
                     "
     gene            1041..5675
                     /locus_tag="PK576_07575"
     CDS             1041..5675
                     /locus_tag="PK576_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOE61489.1"
                     /translation="MVRNGQRQGCGAPASDKVRRLAGPVWAHTVAAVFALALVAGRAH
                     AFRAEEGQTLLRSPVLRERRADRAGVRTAAQRTAQARAERAGVEVVWNDAEGVPISVR
                     GSDLGRTQALGGGRGLKPQGQKAYAADAIAALDDAAMLFGITDAAAEFTVRTLGSPDR
                     LGMSHVRLNQRVNGLRVVGTELIVHFDALDRVYEISGQYVPGIAAAPEPGIDARQALR
                     IAQADLAAMGYPAGTPEQEPERVIYAREVEPQPAYEIALRVRDQAAGLHALWRYWIDT
                     AQGVILVRYNDVKRVAPPSENGMEETITGTRLEREGGEVMDVQGWNEGNGLYYLYNPT
                     ALWCILNASTFDFFDYPDMGTYAYRESNDWGESDPVEISAACNVDAIQNYYQQVHGRN
                     SYDGKGGMADAYVHEGLFYANAYWDGTAMHIGDGDGTSCDSLAVLDIFAHEYTHAVTD
                     HTCDLVYQNESGALNESFSDIFGVCVEFANQPDGRDAYPDAIPGHADWLMGEDSWLEA
                     TALRDLRDPANPETVGAGNELPKLYMGTHWYTGMGDNGGVHYNCGVQNHFFYLLCEGG
                     SGTNDTSAYGYNINGIGVQAGEQIAYRALSKYCVPDDNYKTIMQRWVSAARDLDPRWV
                     RSTRAAWRAVLGMEPPPPMAVTSPSVLPDGRVGSWYSYLLKAKDGIAPYTWALLPEAP
                     KPDWLELAEDGTVSGLPPFEAVGTNEFTVTVVDVAGDVATNRFTFLIRNPFAIPYAES
                     FENNGQSPDSWTQSAVLNQQPWVFANGSPYGHPNSPYDGVYCARLAVLETRLTNSVTR
                     LETPMIDFGTDARAGRLTFRHIMANWEGGQDQLRVYYKNGYFEPWQLLAEFTDATENW
                     TLRSVDLPNLTRSYYLAFEGTANYGYGIHLDEVRIFDPTVPLDFATPEQLPDAYTERP
                     YTVALSVINGVGPFTFELVDGALPTGMVLSADGVISGTVDTVQSALFTVRVTDSQEPL
                     ATAERTFSLNVDLPVATLFFEDFENSGNLPAGWTQEYVERAVNWVCQKGGGNIIQYNQ
                     PSEAHSGGYNATLFQPTWDVYALNNNHITRLVTPPINLGEAPGSIRLVFWHCMIAFED
                     GQDELRVYYKNSAAGAWTLLAEYTENVPVWTQRTLQLPNPTATYYLAFEGNARFGHGV
                     CIDDVLIHDGASAPVITTDAWLPEATVGLPYTQTLTASGGVEPYTWAVVSNTLPVGLT
                     FSTNGVISGTPGSAGTTVFRVAVTGGDGYASTNLLTLRVKALPAGNFTETFDAGTGLP
                     EGWTQQITKGSFGWTFRKGSPENQPAAAHSPQTNACFYANGASYGNAIARLMTPVLNL
                     GTGTTNATLTFWEYRAADGASRDWLKLYYRNTLDGQWIWLTNFTETASSWTQRTVALP
                     ATSPTYALAFEGFSNSGYGIGLDDVEISGDFAGYASWQSTHFTPEELNDPAISGDEAD
                     PDHDGIPNGMEYAMGLDPWSPDAEGLIWGGIIDSYLTLTFRMGKQAFAAGTFYTVEAC
                     TNLLDQVWTVDQVTEYRRDDSNEWWQAFFRHDVPVTNAPQRFLRLRVTLP"
     gene            complement(5705..6802)
                     /locus_tag="PK576_07580"
     CDS             complement(5705..6802)
                     /locus_tag="PK576_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOE61490.1"
                     /translation="MTPPRHLPASEFRNRIRRLLARQRTLRLDAILLVTPVNRYYFTG
                     LDASNGVLLTEPGQTPSFHTDSRYLTMARRLAPWLPCRALWRPADEPAVLAGMGAGWR
                     RIGFEGRMDAARFLRLRAALPDAEWVDVTAETDRLRAVKSPAEQARLRAAVEANDRLF
                     AKLLRGIRPGMSEWTIRTLARREADRMGQGEAFDTIVCAGRNAAEPHHNPDDTLLRRS
                     QPLLIDLGLKLDHYCSDMTRCTVFGTPSRLFRELHGIVLKANRRAVRAIRPGRLCRDI
                     DALARDTIARAGYGAFFGHGLGHGLGLEVHETPSLSPACATVLEPGMVVTVEPGIYLP
                     GRLGVRIEDVVLVTGSGCEVLTRTPHQIGWS"
     gene            complement(6799..7737)
                     /locus_tag="PK576_07585"
     CDS             complement(6799..7737)
                     /locus_tag="PK576_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOE61491.1"
                     /translation="METWFDANLNTLTEALSPCKNCPVANTERDVNLPGTGAVIAVLD
                     SLLAVLYPGCHGQSPVPHENGPAHLKDTLREIALRLHDQTHRAFEYACRNAKCEDCRD
                     CEAHARQAVTALLSGLPAIQNMLQEDIQAAYEGDPAAMSLMEIVMSYPGLYAITVQRI
                     AHALYEQGVPLIPRVMSEHAHSRTGIDIHPGAVIGPGFFIDHGTGVVIGETCRIGRRV
                     KIYQGVTLGALSFAKDEVTGMLVKGIKRHPDVEDNVVIYAGATILGGDTVIGHDSVIG
                     GNVWLIHSVPPGSKVYNQTPKPMIQTGSSIQNDYTV"
     gene            complement(7739..9583)
                     /locus_tag="PK576_07590"
     CDS             complement(7739..9583)
                     /locus_tag="PK576_07590"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOE61492.1"
                     /translation="MDKNTHTTTEIGVVTTQSVRLELPADGFRLEKGGVLREITVAYE
                     RCGAITAANDNVIFICHALTGDAHVAGLRPGESRPSGWWEGMIGPGKGIDTRRYQVVC
                     ANILGGCKGTTGPSSINPDTGKPYGSAFPKITVGDIVEVHRMALRQIGITRLAGVMGG
                     SFGGMQVLEWLIRYPHEIERGIVIASAASLNSQALAFDIVGRHAIIGDPNWQTGDYYD
                     SENLPDKGLSSARKLAHITYLSQQLMDSKFGREKRPEWLTADSDFRNRIEREFGTFFQ
                     VESYLQHQGEKFTQRFDANSYLHITLAMDEYDIEERYGSLDKAFAAVASRVLIVSLSG
                     DWLFMPEQSEELVTALVRQKKRVSYCHLKVAAGHDAFLTHTEELKRVIQAFVPPATES
                     GEAAPIRQWQLRHYQQVINLIPPGSRVLDLGCGNGSLLNILRQLHGCSGNGVEIDLQQ
                     VISAISIGNDVLLEDIDDGLAMIPDGSFDVVVLSETLQTIKRPRELLQQILRVAREAV
                     ISFPNFACFEVRRELLLKGRMPKGKQLPFEWYNTPNIHLFTLKDFRDLCRKDNIQILD
                     LVCRGRGFVERLLLSLGWNNLGASQVIVRIAQGSEDAAPPPPNPTGKG"
BASE COUNT         1729 a         2989 c         3243 g         1780 t
ORIGIN      
        1 tccgggccgt ggtgaagtat ttcggtccgg gtaagaacct gaccgacaag gtcaaggtgc
       61 aggcgttcca caacgccgcg ttcagcggca tgccggtggc ggagtacacc ctcgcgggcg
      121 gaaacctcgc ggccgtgacc aatacggcga atgtggtcac caacgccata atctgcggtc
      181 tggcgcccag tgccgaggcc ggcgattact acctgcgcgc ctacatcgac agcaacggaa
      241 acaacgtgcg ggatgtctgg gaatcgtggg ggtatgccaa cttctacggc gtcagcgaca
      301 aaccgttcga tccgcggccg gtcacggtcc agttcgtgcc gctggcggaa agcccgacgg
      361 taacgatcct gatcgaggat gccgacacgg atcaggactg gttcccggat atctacgagt
      421 acgagacgaa tccgggcgtc ggcttcttgt cgaagtccgg tccgtccacg gggagtgacg
      481 gcgaccctga cttcgagatc aatccgaatc tgggcgacac gaccgggatc aacacgctct
      541 tctcggccct gatgttaggc agcacggacg ccgacggtga cgaggtgggt gatctggcgg
      601 aactgatcct cggttcggac ggccagaagg tctccacttc cggtgacggg gtttccgacg
      661 gtctcaagat cggtctgggg ctctcgccgg ccgacacgct ggcactggag atgaccggac
      721 tggaactcac cggcagcgag gcgaccctca cctggaaact ggtggtggat caggctgaag
      781 gggtgaaccg caacctgatc aagagccttg ccggcgcgcc cggaccggcg acctacgaag
      841 tgctcttcaa gccttcgctg gatgcggccg actggcaggt ggtggccacg ggcacggcca
      901 cgctttcgga aagtgaagag ggcgtgagcg cagcgatcag ccgtgtgcag caggcattgc
      961 aggatgcgcc gacacagggc ttcttccgag tccgtctggt ggagtaacgg gaacggcgga
     1021 gtatacgcgg agtttcgcac gtggtaagaa acggacagcg tcagggatgt ggggcaccgg
     1081 cctcagacaa ggtccgccgg ttggcgggcc ctgtctgggc gcatacggtg gcggcggtgt
     1141 tcgccctggc cctcgtcgcg ggaagggcgc atgcgttccg ggccgaggag gggcagacct
     1201 tgttgcggtc gcccgtgttg cgtgagcggc gcgctgaccg tgcgggggtg cgcacggcgg
     1261 cgcaacggac ggcgcaggcc cgggccgaac gcgcgggtgt cgaggtggtg tggaacgacg
     1321 ccgaaggcgt gccgatctcg gtccgcggct cggatctcgg gcgaacgcag gctttgggcg
     1381 gcggcagggg gctcaagccc caggggcaga aggcgtatgc tgcggatgcg atcgcggcgc
     1441 tggatgacgc cgcgatgctt ttcggcatca cggatgcggc ggcggagttc acggtccgca
     1501 cccttggttc gccggaccgg ctcgggatgt cgcatgtgcg gctcaaccag cgggtcaacg
     1561 gtctgcgggt ggtcggcacg gaactgatcg tgcactttga cgcgctggac cgcgtctatg
     1621 agatcagcgg tcagtatgtg cccgggatcg cggccgcgcc cgaacccgga atcgacgcgc
     1681 ggcaggccct gcggatcgcg caggccgatc tggcggccat gggctatcct gcgggaacgc
     1741 cggagcagga gcccgagcgg gtgatctacg cccgcgaagt cgagccgcaa ccggcctatg
     1801 agatcgcgtt gcgcgtgcgg gatcaggccg ccggacttca cgccctctgg cgctattgga
     1861 ttgatacggc gcagggtgtg atccttgtcc gctacaacga cgtcaaacgg gtcgcgcctc
     1921 cgagtgagaa cgggatggag gagacgatca ccggcacccg cctcgagcgc gaggggggcg
     1981 aggtgatgga tgtgcagggg tggaatgagg gaaacggctt gtactacctc tacaacccga
     2041 ccgcgctctg gtgcatcttg aacgcttcga cgttcgactt cttcgactat cccgatatgg
     2101 ggacctacgc ttaccgcgaa tcgaacgact ggggggaatc ggatccggtc gagatttccg
     2161 ccgcctgtaa tgtggatgcg atccagaatt actaccagca ggttcacgga cgcaacagtt
     2221 acgacggaaa aggcggcatg gccgatgcgt atgtgcatga ggggttgttt tacgccaatg
     2281 cctattggga cggaaccgcg atgcatatcg gcgacggcga cggcacgagt tgtgatagtc
     2341 tcgcggtgct cgacattttc gcccatgaat acacccatgc cgtgacggac cacacctgcg
     2401 atctcgtgta tcaaaacgag tccggcgcgc tgaacgagtc cttttccgat attttcggtg
     2461 tttgcgtcga attcgcgaac cagccggacg gacgcgatgc gtatcccgac gcgattccgg
     2521 gtcatgccga ctggctgatg ggtgaggact cctggctgga agccaccgcg ctgcgcgacc
     2581 tgcgcgaccc ggccaatccc gaaacggtcg gcgcgggcaa tgaactgccc aaactctata
     2641 tgggaaccca ttggtacacg ggtatgggcg acaacggcgg ggtgcactac aactgcggcg
     2701 tgcagaacca cttcttctat ctgctctgcg agggcggatc cggaacgaac gacacttccg
     2761 cctatggcta taacatcaac gggatcggcg tgcaggccgg cgaacagatc gcctaccgcg
     2821 cgctgtccaa gtattgcgtg ccggacgaca actacaaaac cattatgcag cggtgggtct
     2881 ctgcggcgcg ggatcttgac ccccgttggg tgcgcagcac gcgggcggcc tggagggcgg
     2941 ttctgggcat ggaaccgccg cctccgatgg cagttacctc gccgtccgtg ctgccggatg
     3001 gccgggtcgg ttcttggtac agctatctgc tcaaggccaa agacgggatc gcgccgtata
     3061 cctgggcact gcttccggaa gcgcctaagc ccgactggct ggaactcgcg gaggacggaa
     3121 cggtctcggg gctgccgccg ttcgaagcgg tcggcacaaa cgagtttacg gtgacggtgg
     3181 tggatgtcgc gggtgatgtc gcgaccaacc gtttcacgtt cctgatccgc aaccctttcg
     3241 ccattccgta cgcggagtcc tttgaaaaca acggccagag ccccgacagt tggacgcaga
     3301 gcgcggttct gaatcagcag ccctgggttt tcgcgaacgg cagcccctac ggccatccca
     3361 acagcccgta cgacggcgtc tactgcgccc ggctggcggt gctggagacg cggttgacca
     3421 actccgtcac gcgcctggag acgccgatga tcgatttcgg caccgacgcc cgcgcgggcc
     3481 ggctgacttt ccgtcacatt atggccaact gggagggcgg tcaggaccag ttgcgcgtgt
     3541 attacaaaaa cgggtatttc gagccgtggc agcttctggc cgagttcacc gacgccaccg
     3601 agaactggac cctgcgcagt gtggatctgc cgaacctgac ccgctcctac tatctggcct
     3661 tcgagggcac ggccaattac ggatacggca ttcatctcga cgaggtccgg atcttcgatc
     3721 cgaccgtgcc gctggatttc gccacgccgg aacagttgcc ggacgcctac acggaaagac
     3781 cctacacggt tgcgttgtcc gtgatcaacg gcgtgggccc gttcaccttt gagctggtcg
     3841 acggcgcact gccgaccggg atggtcctga gtgccgacgg tgtgatcagc ggaacggttg
     3901 acacggttca gagcgccctc ttcaccgtgc gggtgaccga ttcgcaggaa cctctggcca
     3961 cggctgaacg gactttcagt ctcaatgtgg acctgccggt cgcgacgctt ttcttcgagg
     4021 atttcgagaa cagcggcaat ctgccggcgg gctggacaca ggagtatgtg gagcgcgccg
     4081 tgaactgggt gtgccagaaa ggcggcggca acatcatcca gtacaaccag ccttcggagg
     4141 cccacagcgg cggctacaac gccacgttgt tccagcccac atgggatgtg tacgccctga
     4201 acaacaacca catcacccgg ctggtcacgc cgccgatcaa tctgggcgag gcgccgggca
     4261 gcatccgtct ggtgttctgg cactgcatga tcgcgttcga ggacggccag gatgagctgc
     4321 gggtctacta caagaactcg gccgccgggg cgtggaccct gctggcggag tataccgaga
     4381 atgtgccggt ctggacccag cggacactcc agcttcccaa ccccaccgcg acgtattatc
     4441 tggctttcga gggcaacgcg cgtttcggcc acggcgtgtg catcgacgat gtcctgattc
     4501 acgacggcgc gagcgcgccg gtcatcacga ccgacgcatg gctccccgaa gccacggtgg
     4561 ggctgccgta cacgcagacc ctgacggctt ccggcggggt ggagccctac acttgggccg
     4621 tggtctcgaa caccttgccc gtaggcctca ccttcagcac caacggggtg atcagcggta
     4681 cgcccggaag cgcgggcaca accgtgttcc gtgtggcggt gaccggcggg gacggctatg
     4741 cctcgaccaa tcttctcacc ctgcgggtga aagccctgcc cgcagggaac ttcaccgaga
     4801 cctttgatgc cggaaccggt cttcctgagg gctggaccca gcaaatcacg aaaggttcgt
     4861 tcggctggac cttccgcaag ggcagtccgg aaaaccagcc cgcggcagcc cactcgcccc
     4921 aaaccaacgc ctgtttttac gccaacggcg catcgtacgg gaacgccatc gcccgactca
     4981 tgaccccggt gctcaatttg ggcaccggca caaccaacgc gacgctcacc ttctgggagt
     5041 accgggctgc ggatggggca tcccgcgact ggctgaagct gtactaccgg aacacgcttg
     5101 acggccagtg gatttggttg acgaatttca cggagaccgc ttcctcgtgg acccagcgca
     5161 cggtggcact gcccgcgacc agcccgacct acgcgctggc ctttgaaggg ttttccaaca
     5221 gcgggtatgg catcggtctg gacgatgttg aaatcagcgg cgatttcgcc ggatacgcct
     5281 cttggcagag tacgcatttc actccggaag agctgaacga tcccgcgatt tccggcgatg
     5341 aggccgatcc ggaccatgac gggattccga acggcatgga gtacgccatg ggtttggatc
     5401 cgtggtcgcc cgacgcggag ggtctgatct ggggcgggat catcgactcc tatctcacgc
     5461 tcaccttccg catgggcaag caggcgtttg ccgcaggcac gttctatacg gttgaagcct
     5521 gcaccaattt gttggatcag gtctggaccg ttgaccaggt cacggagtat cggcgcgatg
     5581 acagcaatga gtggtggcag gccttcttcc gccacgatgt gccggtcacc aacgcgcccc
     5641 agcgcttcct gcgtctacgg gttacactgc cttgaccttt ttgcgggccc gttcgggctt
     5701 ggtctcagct ccagccgatt tgatgcggcg tgcgggtgag cacctcacag ccgctaccgg
     5761 tcacgagcac gacatcctcg atgcgtaccc ccagccgtcc cggcaggtag atgccgggtt
     5821 ccacggtcac caccatgccc ggttcgagga ctgtcgcgca tgcgggtgag aggctggggg
     5881 tttcgtggac ctccagtccc agaccgtgac ccaggccgtg accgaaaaaa gcgccgtaac
     5941 ccgcccgcgc gatcgtatcc cgtgcgagcg cgtcgatgtc gcggcagagg cgtcccggcc
     6001 ggatggcccg cacggcccgg cggttggcct tcagcacgat gccatgaagt tcacggaaga
     6061 gccgggaggg tgtgccgaag acggtacagc gggtcatatc cgagcagtag tggtccagtt
     6121 tgagcccgag atcgatcagc aacggttggc tgcggcgcag aagcgtgtca tccgggttgt
     6181 ggtggggttc cgcggcgttg cgacccgcgc agacgatcgt gtcgaacgcc tcgccctgac
     6241 ccatccggtc agcctcccgg cgggccagcg tgcggatcgt ccactcgctc atgccgggcc
     6301 ggatcccgcg cagcagtttc gcgaacaggc ggtcgttcgc ctcgactgcc gcgcgcagcc
     6361 gggcctgttc ggcaggggat ttgacggcgc ggaggcgatc cgtctccgcc gtcacgtcaa
     6421 cccactccgc atccggcagc gcggcacgga ggcgcaggaa gcgcgcggcg tccatgcgcc
     6481 cctcgaagcc gatgcggcgc catccggcac ccatgccggc cagcacggcg ggttcgtcgg
     6541 cgggacgcca cagtgcgcgg cagggcagcc agggcgccag acgtcgcgcc atggtgaggt
     6601 atcgggaatc cgtgtgaaag gagggggtct gacccggctc ggtcaacaac acgccgttcg
     6661 aggcatcgag gccggtgaag taatagcggt tgaccggagt gaccagcagg atcgcatcca
     6721 gccgcagtgt gcgctggcgg gccagcagac gtcggatgcg gttacggaac tcggaggctg
     6781 ggaggtggcg cggcggcgtc ataccgtgta atcgttctgg atcgaagacc cggtttggat
     6841 catcggcttg ggtgtctggt tgtagacctt ggaacccggc ggcaccgagt ggatcagcca
     6901 gacgttgccg ccgatcacgg agtcatggcc gatcaccgtg tcgccgccga gaatggtggc
     6961 ccccgcatag atcaccacgt tgtcctcgac atccgggtgg cgtttgatgc ctttgaccag
     7021 catgcccgtg acctcgtctt tggcgaaact gagcgcgccg agcgtgaccc cctggtagat
     7081 tttgacgcgg cgtccgatac ggcaggtctc gccgatcacc acgccggtgc cgtggtcgat
     7141 aaaaaagccg ggtccgatca cggcgccggg gtggatatcg atgccggtgc gcgagtgggc
     7201 gtgttcgctc atcacgcgcg ggatcagcgg gacgccctgc tcgtagagcg cgtgggcgat
     7261 gcgctggacc gtgatcgcgt acagccccgg ataactcatc acgatctcca tgagggacat
     7321 cgcggccggg tcgccctcgt aggcggcctg gatatcctcc tgaagcatat tctggatcgc
     7381 gggcaggccg cttagcagcg ctgtgaccgc ctgacgggcg tgtgcttcac aatcgcggca
     7441 atcctcgcat ttggcgtttc ggcaggcata ctcgaaggcg cggtgcgtct gatcgtgcag
     7501 ccgcagggcg atttcgcgca gggtgtcctt caggtgcgcg ggtccgttct cgtgcggcac
     7561 gggggattgg ccgtggcagc cggggtagag cacggccaga agcgagtcca gcacggcgat
     7621 caccgcaccg gtgcccggca ggttgacatc gcgttccgtg ttggcgaccg ggcaattctt
     7681 gcagggggag agcgcttcgg tcagggtgtt gagattcgcg tcaaaccagg tttccatatc
     7741 agccctttcc ggttgggttg gggggcggcg gggcggcgtc ttcgctgcct tgggcgatac
     7801 gtacgatcac ctgcgaggca ccgaggttgt tccagcccag agagagcagg agccgctcca
     7861 cgaatccgcg gccccggcag accagatcga ggatctggat gttgtccttt cggcagaggt
     7921 cgcggaagtc tttgagggtg aagaggtgga tgttgggcgt gttgtaccat tcgaagggca
     7981 actgcttacc tttgggcatg cggcctttga gcagcagctc gcgccgcacc tcgaagcagg
     8041 cgaagttcgg gaaggagatc accgcctcgc gcgccacgcg cagaatctgc tggagcagct
     8101 cacgcggccg cttgatggtc tgcaacgttt cgctgagcac caccacgtcg aagctgccgt
     8161 ccggaatcat ggcgagcccg tcgtcgatat cctcaagcag cacatcgttc cctatgctga
     8221 tggcggagat gacctgctgg aggtcgatct cgacaccgtt gccgctgcag ccgtggagtt
     8281 gccgcaggat gttcagcagc gacccgttgc cgcaacccag atccagcacg cggctgcccg
     8341 gcgggatcag attgatcacc tgctggtaat ggcgcaactg ccactgccgg atcggcgcgg
     8401 cttcgccgga ctccgtcgcg ggcggcacga aggcctggat cacgcgcttc aactcctcgg
     8461 tgtgcgtcag aaacgcgtca tgtccggcag ccactttgag gtggcagtag gatacccgct
     8521 tcttctggcg cacgagcgcg gtgaccagtt cctcggattg ttcgggcatg aagagccagt
     8581 cgccgctgag cgagacgatc agcacgcgcg aggccacggc ggcgaacgcc ttgtccagcg
     8641 aaccgtagcg ctcctcgatg tcgtactcgt ccatggccag cgtgatgtgc aggtaggagt
     8701 tggcgtcgaa gcgctgggtg aacttctctc cctgatgctg cagataactc tcgacttgaa
     8761 agaacgtgcc gaattcgcgc tcgatgcggt tccggaagtc ggaatcggcc gtgagccact
     8821 cggggcgctt ctcgcggccg aacttggagt ccatgagctg ttgcgacaga taggtgatgt
     8881 gcgccagttt gcgcgcgctg gaaagcccct tgtcgggcag attctcgctg tcgtagtaat
     8941 cacccgtctg ccagttcggg tcgccgatga tcgcgtgccg accaacgatg tcaaaggcca
     9001 gcgcctggga gttcaggctc gccgcggaag cgatcacgat tccccgctcg atctcgtgcg
     9061 ggtaacggat cagccactcc agcacctgca tgccgccgaa actgccgccc atcacacccg
     9121 ccagccgcgt gatgccgatt tggcgcaggg ccatgcggtg gacctccacg atgtcgccca
     9181 ccgtgatctt cggaaaggcg gagccgtagg gttttccggt gtcggggttg atcgaggagg
     9241 gcccggtggt gcctttgcac ccgccgagga tgttcgcgca cacgacctga tagcgccggg
     9301 tgtcgatccc cttgcccggg ccgatcatgc cctcccacca gccgctcggc ctgctctcgc
     9361 ccggacgcaa gcccgccaca tgggcgtcgc cggtgagcgc gtggcagatg aagatcacat
     9421 tgtcgttggc cgcggtgatc gcgccgcagc gctcataggc caccgtgatc tcccgcagca
     9481 cgccgccttt ttcgaggcgg aatccgtctg cgggaagctc cagccggacg ctctgcgtcg
     9541 ttaccacacc gatttctgtt gtcgtatgcg tgtttttgtc catgttcctt tcagaaaacg
     9601 gggaaagtct aacatggcgc actctgtccg gcaattctcc ttttgccgga ctgcgggcgt
     9661 tcccgcccgt tggtagggac ggttctccga accgtccgcg gacgccttaa aaagagcgtt
     9721 gaaagcaacg ctctttggtt t
//
LOCUS       DAOSLE010000101         9712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6770_length_9712_cov_7.380760, whole genome shotgun sequence.
ACCESSION   DAOSLE010000101 DAOSLE010000000
VERSION     DAOSLE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_9712_cov_7.380760"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..3734)
                     /locus_tag="PK576_07595"
     CDS             complement(201..3734)
                     /locus_tag="PK576_07595"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE61493.1"
                     /translation="MTPSRKSIVLLSAALAVWAAFAQSVIEIEMPWPKEGTGFPCGHP
                     VDVSIRHTLDGKATAAKGVKIAFDNFGPKTVRAEETCDLPEQGLRVKGLTLNEPGFLR
                     CRVTVGGKNYVQSVPFEPERIVKGSPTPPDFMTYWQREIAKLDAESSDEPELKPWRGP
                     DTNQWDAWRVSFATAGGKRVYGLLSVPKNLSEGPFPVHVSVPGAGQPSKTYSASWIYS
                     QTPERNTVRLTMFAHCAPIDDAKEREAYFTRIRAEHAEKYGVGYYPQAGISESREAYH
                     FHPIILGANRAVNWLARQSYCDTNRFTYSGSSQGGGFGIILAGLNRHFTRVFADVPAM
                     ADTMGARAGRQSGWPQPVETQRPENRSAAERFAPYFDGANFARYVTCPIRVTCGCKDT
                     SCAPPAVYAAYNEIPSQDKQIFVMPHNGHESNGKIKNQAHAWRQGTGERGLAPFDTLT
                     CEVTGVAFGSGANTDPTFKVPKLVAAIRRTDGVIEDVPLRAVNWMGAYLVDRRFSKSE
                     RERFGTKAELVGVRLEGGAPETNAKIKIENATMFKEVLNPLSIQVTPRDALPFPNRAD
                     TIVPDTPEPAAGDLLAEWNASLPPGCAVSKRRVGKSLVIDITAPAGKVTEIPLGVARE
                     AKKVKSFAIPYLTWGERNGRVHADLLEGGWFRLAEFDWYASNASDVYARETEGGRELV
                     ARYKPKTDGTFNPVRERIVITLSNVFEEILPEIPNPASPWKHVTGMHVWRSHPSYDRA
                     EDLALWRQVKAAGIKHVAVTDHETMWRDGGEPFTFCTDAAKGKGGDEAQKAYTRAMID
                     ELGFLYGPYNNFTDLATQSEFFNRDTVGRWSDGSFHNAWMRCYGPKPVLAPLACDRFA
                     GAIQRKFGFNTAYCDVHTSMLPWQRTDYDHRVPGAATYAQTYYSWGETLLKQKKIWNG
                     PVYSEGAHHFMWAGLADGSYAQDRSYDFRNEPWIVEFELLRTQRLCTDFGMGTLSMFS
                     PARTLHERLYYLPYQPQGRDELVNRFLAATIAFGHSGYLILDKCWEPDKPFGRAYGVK
                     SKPVWQEKGLPAETLRSYFMIQALAARYTQSHAVEIRYVDAEGRLHAASEAIRNGTVA
                     RNQIVTRYADGTVTAVNGSDTLPMKTSAHGRKIELAPNGFAGWSSGVTVLSTDTDGRR
                     VHYAEGPDYVYREIEGRKPCVSLTSRHETKP"
     gene            complement(3745..5739)
                     /locus_tag="PK576_07600"
     CDS             complement(3745..5739)
                     /locus_tag="PK576_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61494.1"
                     /translation="MRTVLIVSVALGVIGAFADDQWTLNSDTPTWWFEPAAWSNGTYP
                     QSGESVIIGASSDNAKANVYLQSGDVVSGIADLTFGKNTGAQTHLTIPTGASLTFTNT
                     MRLMGQSVQKATGLVEITGGTLKGKAFNWGGHTSSKGTVRITNNGRAEFTSCTSGWLG
                     PSDFEIDTGHLCVGSGNFLFGNRTDAYARLFGRNATIETTAGIFSIGANVSGALGAWF
                     LEDSAVTVSGSGGIVLGRASQSCLRITNTVVRFTHASAPFTLGQDTNNTVTAGVWSDF
                     EMVGENAMVSNSYRLKLFNNARYIQRGGQTILRANNTGDQIEINGVPGTEQLVEVHGG
                     TFSLTNANPNGDARIILGGSARAMFRQTDGTVTAPGILFKPCGPDNARYEMTGGTLEL
                     DDSKGGNGGIRTSDKSTRAGTFSIIGGSPNIRMTRIGTYNSTDYQPMIEYVICTNFVA
                     PICFSRPSQSYYSWVSGIYTIVPQGGMQLIHTNTVMLLDGRDGEFGILPQNPGSGGTF
                     LPNPALWESGTTDLYGGSKADRLGSRLLASAAFESGAAPATPVSCGFLKLPKTSQKGY
                     TVWLNLLPQNEVTIAELVADLTAAGYPAQADASREGYNVRAAIPENLVAHGSDDEYLV
                     FDFARHMTPEDVRNGTPTVRALVGRVLAEKRTGGFAILIN"
     gene            complement(6076..6741)
                     /locus_tag="PK576_07605"
     CDS             complement(6076..6741)
                     /locus_tag="PK576_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61495.1"
                     /translation="MQKLFLLALALTCAVTVRSEMLVKSEPIRPDRKITLFNGRDLSG
                     WTKVITAEPGACPDATWSVADGVIRCTGKPFGYLMTQQSYADYRLHVEYRWYGTSDQM
                     NSGIFIHKTGPDTFFLPKAIETQLKKDNAGDFVLLSQATLNNLTNPKNLRVEKIAPCS
                     EKPVGEWNRVEITVRGNTLSSYINGVLQNSGNAIYTDAGQICLQSEGGPIEFRNITIE
                     PIP"
     gene            complement(7067..7900)
                     /locus_tag="PK576_07610"
     CDS             complement(7067..7900)
                     /locus_tag="PK576_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE61496.1"
                     /translation="MESLLPNYDGVNIEWDQRSIHSEQVTFGQVRYQPGGYCGPRVQR
                     DFELALLYSGSCCVRLDGQPRELRVGHVYLFKPRRREHFLFDPQTPTHHFWCSVTPGY
                     LPPALRHALEATPDEGHLPSECFNRLISAAFLLRTVQSPTSLQVVDTLALTLFWEFLN
                     LADHDTLMIREDACVTRALRYMEEHLGESDCFVGAQRAARCSENALIYKFRRALGTTP
                     ARHLWRLRTEKGLALLAETGLSVTEIADQCGFKNPFHFSRCVRRMQGQPPREIRRRAW
                     S"
     gene            8124..9695
                     /locus_tag="PK576_07615"
     CDS             8124..9695
                     /locus_tag="PK576_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61497.1"
                     /translation="MSMFMGKTPCSGCRLERRDFLAAAGCTACAATLGRVMAAAPARA
                     AEKAPDLRIRILFALHAPVQPKPDWPNIGFNFQPVMDQFVEGLRTHCPGFDFVTSMAA
                     NEEEAKAIVAADQGSRIDGYIVYQLNCWNRVVQPAIATGKPTLFVDFAYGGSGGMLVY
                     TSGFLIYKVPNVGFVSSSRFEDVAAAVQCFAQVKRGGTDFATLTAACRRKLTPQAGDL
                     SCVNDPVEVLSAKDCLAKMREAKLLVVTNKSVGPYPAIKEQMGIEVVQIPFEELNTAW
                     KAADKDQARAVADRWQKDARAIIGVSRETLENSAAMYLGQKALLKKHGANGLTVNCLG
                     GFYGGHIHAYPCMGFHELVNEGLIGGCEADVRSAVSMIAMTTLTQGRPGFISDPVIDT
                     SKRQIIYAHCVASNKPFGPQGPSNPFEIMTHSEDRKGAAVRSLFPVGYMTTTVEFDAN
                     RKTVLFHQAKAVANVDDDRACRTKLAAEPVGDMEKLFTHWNRWGWHRVTVFGDLKEPV
                     FALADAMGWKVVQEA"
BASE COUNT         1847 a         2820 c         3041 g         2004 t
ORIGIN      
        1 tttcagggtt tcatccaaat cggggtcggg gtcgggatcg ctatcgggat cgattcttcc
       61 accacgggac actttcttgg gtttcagggt ttcagggttt cagggtttct ggtttcaggg
      121 tttcatccaa atcggggtcg gggtcgggat cgctatcggg atcgattctt ccaccacggg
      181 acacaagaac acacggaaag tcacggcttt gtttcatgcc gcgacgtgag cgagacgcac
      241 ggctttcggc cttcgatctc gcgatagaca tagtcgggac cttcggcgta atggacgcgg
      301 cgtccgtcgg tatcggtgga gagcacggtg acgccgctgg accatcccgc gaaaccgttg
      361 ggcgcaagct cgatcttgcg tccgtgcgcg gacgtcttca tcggcaaagt gtcgctgccg
      421 ttcactgcgg tgaccgtgcc gtccgcataa cgtgtgacga tctgattgcg ggccacggtt
      481 ccattccgga tcgcctcgga ggcggcatgt aaccgcccct cggcgtccac gtaccggatc
      541 tcgacggcgt gcgattgcgt gtaccgggcc gcgagcgcct gaatcatgaa gtaactgcgg
      601 agcgtctcgg cgggaaggcc cttttcctgc cagaccggtt tgctttttac cccgtatgcg
      661 cgcccgaacg gcttatccgg ctcccagcat ttgtcgagga tcaggtaacc ggaatggccg
      721 aacgcgattg tcgcggcgag gaaacggttc acgagttcgt cacgaccttg gggctggtac
      781 ggcagataat acagccgttc gtgaagagtt cgcgcaggcg aaaacatcga gagcgttccc
      841 atgccgaagt cggtgcaaag acgctgtgtg cggagcagtt cgaactcgac gatccatggt
      901 tcattacgga aatcgtagct ccggtcctgc gcgtaggagc catccgcaag accggcccac
      961 atgaagtggt gcgccccttc ggaatagacg ggaccgttcc agatcttctt ctgtttcaag
     1021 agcgtctcgc cccacgaata ataggtctgc gcgtaggttg ccgcgccggg aacacggtgg
     1081 tcgtagtccg tgcgctgcca cggcaacatc gaagtgtgca cgtcgcagta cgctgtgttg
     1141 aaaccgaact tacgctggat cgcgccagcg aaccggtcgc aggcgagcgg tgcgagcacg
     1201 ggtttcggac cgtagcaccg catccaggcg ttgtggaacg atccgtccga ccatcgtccc
     1261 accgtgtcgc gattgaagaa ttcgctctgc gtcgcgaggt ccgtgaagtt gttgtacgga
     1321 ccataaagga agccgagttc atcgatcatc gcgcgcgtgt aggccttctg tgcctcgtcg
     1381 ccgcccttcc cctttgccgc gtcggtgcag aaggtgaacg gttcgccgcc gtcccgccac
     1441 atcgtctcat ggtccgtcac ggcgacgtgc ttgattccgg cggcctttac ctgccgccag
     1501 agcgccagat cttccgcacg gtcgtacgat gggtgtgaac gccacacgtg catcccggtg
     1561 acgtgtttcc aaggggacgc cggattgggg atctccggca ggatctcttc gaaaacgttc
     1621 gaaagcgtga tcacgatccg ctcacgcacg gggttgaacg tcccgtccgt cttcggtttg
     1681 taacgcgcca cgagttcgcg gccgccttcc gtctcccgcg cgtacacgtc gctcgcgttc
     1741 gaggcgtacc agtcgaactc cgccagccgg aaccagccgc cttcgagcag gtctgcgtgc
     1801 acgcgtccgt ttcgctcgcc ccaggtcaga taggggatgg cgaagctttt cacctttttc
     1861 gcttcgcgtg caacgcccag cgggatttcc gtgactttgc ccgctggagc cgtgatgtcg
     1921 atcacgagtg atttcccgac gcgccgtttc gacaccgcgc agcccggcgg cagcgaggcg
     1981 ttccattccg cgagcagatc gcccgccgcc ggttcgggcg tgtcgggaac aatggtgtcc
     2041 gcacggttcg ggaacggcag cgcgtcacgc ggcgtcacct gaatcgacaa cggattcagc
     2101 acctccttga acatcgtggc gttttcaatc ttgatcttgg cgttcgtttc cggcgcgccg
     2161 ccttcgagcc gcacacccac cagttcggcc tttgttccaa aacgctcgcg ttccgacttc
     2221 gagaaccgcc ggtccaccag atacgctccc atccaattga cggcccgcag cggaacgtcc
     2281 tcgatgacac catccgtccg ccggatcgct gccacgagct ttggcacttt gaacgtcgga
     2341 tccgtgttgg cgccggatcc gaacgcgacg ccggtcacct cgcatgtgag cgtgtcgaac
     2401 ggcgccagtc cacgttcccc tgtgccttgt cgccaggcat gcgcctgatt cttgatcttg
     2461 ccattgctct catgcccgtt atggggcatg acgaagatct gtttgtcctg agatggaatc
     2521 tcgttatagg cggcgtaaac ggccggtggc gcgcagctcg tgtctttgca accgcaggtg
     2581 acgcggatcg ggcaggtgac gtagcgcgcg aaattggccc cgtcgaagta cggggcgaac
     2641 ctctctgcgg cggaacggtt ctcggggcgc tgcgtttcga cgggttgcgg ccagccgctt
     2701 tgtcgtcccg cgcgtgcccc catcgtatcc gccatcgccg gaacatcagc aaagacgcgt
     2761 gtgaaatggc ggttgaggcc tgcgaggatg atgccgaagc cccccccctg cgaacttccg
     2821 gagtaggtga agcgattcgt gtcgcaatag gactgccgcg ccagccagtt cacggcgcgg
     2881 ttggcgccga gaatgatcgg atggaaatga tacgcttcgc gcgattcgga gatgccggcc
     2941 tgcggataat atccgacccc atacttttcg gcgtgctcgg cccggatgcg cgtgaaatac
     3001 gcttcgcgtt ccttggcgtc gtcgatcggt gcgcaatgcg caaacatcgt gaggcgcacc
     3061 gtattccgct ccggcgtctg gctgtatatc cagctcgccg aatacgtctt tgagggctgg
     3121 cccgcgccgg gaacgctcac atgcacgggg aacgggcctt ccgaaaggtt tttcggcacg
     3181 gacaacagtc cgtagacgcg cttcccgcct gccgtcgcga agctgacccg ccaggcgtcc
     3241 cattgattgg tgtccggacc gcgccacggt ttcaactccg gctcgtctga cgactccgcg
     3301 tccagtttgg cgatctcacg ttgccagtac gtcatgaaat ccggcggcgt tggcgatccc
     3361 ttcacgatcc gttccggctc gaacggcacg gactgcacat aatttttacc gccgaccgtg
     3421 acccggcatc gcagaaagcc gggctcgttc agcgtgagac ccttgacgcg aagaccttgt
     3481 tcgggaagat cacaggtttc ttccgcacgc acggttttcg gcccgaagtt gtcaaaggcg
     3541 atcttgacgc ctttggcggc ggtcgccttg ccgtcaaggg tgtggcgtat cgatacgtcg
     3601 acggggtgtc cgcacgggaa gcccgtcccc tcctttggcc aaggcatttc gatctcgata
     3661 accgattgcg cgaatgctgc ccatacggca agggcagcgg acagcaacac gatgctttta
     3721 cgggagggtg tcatgtttgc ttcctcaatt tataaggatg gcgaatcccc cagtccgctt
     3781 ttcggccaaa acgcgtccga cgagcgcgcg gacagttggc gtgccgttcc ggacgtcttc
     3841 cggcgtcata tggcgggcga agtcgaagac aaggtattcg tcgtcggaac cgtgcgcgac
     3901 aaggttttcc ggaatcgccg cccggacgtt gtacccttcg cgggacgcat ccgcttgggc
     3961 gggatatccg gccgcagtca aatcagcaac cagttccgcg atcgtgactt cgttctgcgg
     4021 cagcaggttc agccagaccg tgtagccttt ttgagacgtc ttcggcagtt ttaaaaaccc
     4081 gcacgagacc ggtgtggcgg gcgcggcgcc cgattcgaac gccgccgaag cgagaaggcg
     4141 cgaaccgagc cgatcggcct tgctgccgcc gtagaggtct gtcgtgccgg actcccataa
     4201 cgcggggttc ggcaggaacg tgccgccgga accggggttt tgcggcaaga tgccgaactc
     4261 gccgtcgcgt ccgtccaaca gcatcaccgt attggtatgg atgagttgca tgccgccctg
     4321 cggcacgatc gtgtagatgc cgctcaccca actgtaataa gattgcgacg gacgcgagaa
     4381 acagatcggc gcgacgaagt tggtgcaaat gacgtattcg atcatcggct ggtagtcggt
     4441 cgagttgtac gtgccgatgc gcgtcatccg gatgttcggc gaaccgccga tgatcgagaa
     4501 cgtgcctgcg cgcgtggatt tgtccgaggt gcggatgccg ccattgcccc ccttggagtc
     4561 gtcgagttcg agcgtgccgc cggtcatctc gtaccgcgcg ttgtccgggc cgcagggctt
     4621 gaaaaggatt cccggtgcgg tcaccgtgcc gtccgtctgg cggaacatcg cccgagcgct
     4681 gccgcccaag atgatgcgcg cgtcgccgtt cggattcgcg ttggtgagcg aaaacgtgcc
     4741 gccgtgcacc tcgacaagtt gttccgtgcc ggggacgccg tttatctcga tctgatcgcc
     4801 ggtgttgttt gcccggagga tcgtctgccc cccgcgctga atgtaccgcg cgttgttgaa
     4861 cagtttcagc cggtacgaat tcgagaccat cgcattctcg ccgaccatct cgaagtcgga
     4921 ccacacgccc gcggtcaccg tattgttggt gtcctgtccg agggtgaagg gcgcggacgc
     4981 atgtgtaaag cggactacgg tgttggtgat gcggaggcag gattgcgaag cgcgcccgag
     5041 cacgatgccg ccactccccg agacggtgac agcagaatct tccagaaacc acgctccaag
     5101 agcccccgat acgttggcgc cgatagagaa tatccccgcc gtggtttcaa tggtcgcgtt
     5161 cctgccaaac aaccgggcat acgcatcggt acgattgccg aaaaggaaat tgccggatcc
     5221 gacacacagg tgaccggtgt cgatttcgaa gtccgacgga ccgagccatc ccgacgtgca
     5281 ggaggtgaac tccgcgcggc cgttgtttgt gattcggacg gtacccttcg acgatgtgtg
     5341 tccgccccag ttgaacgctt tgcccttgag cgtgccgccg gtaatttcaa ccagccccgt
     5401 cgctttctgg acagactgcc ccatgagtct catcgtgttc gtgaaggtca gcgaggcgcc
     5461 ggtcggaatc gtgaggtggg tctgcgctcc ggtgttcttg ccgaaagtca ggtcggctat
     5521 gccggaaacc acgtcaccgg attggagata gacattcgct ttcgcgttgt ccgacgatgc
     5581 gccgattatc acggactccc cgctttgcgg gtaggtgccg ttcgaccacg cggccggctc
     5641 gaaccaccat gtcggcgtgt cggagttgag cgtccattga tcgtcagcga atgccccgat
     5701 gacgccgagt gctaccgaaa cgataagaac ggtccgcatg ataatttccc cctatgctaa
     5761 aagccccccc ttttccatta actgtgtaaa accataacac acccccccct tgcgtcacaa
     5821 gaaacgtttg tgcgaacgca ttcctgaccc cctgaccgtc cgtactttgt tcggctcgca
     5881 gaaccgccct caccgtcaaa cagacatttg cctctcccgg acgccgcgac cggcacacat
     5941 acacgaaacc gagtacgaga acgaggagga ggacgggcgc tgatcccgat aacgatgcct
     6001 cagaccggga caatcgtcgg acgggacggg cagacgctcg tcccgcccag ccacaatatc
     6061 gggaggggtg ccggatcagg ggatcggctc gatcgtgatg ttgcggaact cgatggggcc
     6121 gccctcactc tggaggcaga tctggccggc gtcggtatat atcgcgttgc cgctgttctg
     6181 caagacgccg ttgatgtatg aagagagggt attgccccgg accgtgattt caacgcggtt
     6241 ccattcaccg accggtttct cggagcatgg ggcgattttt tcgacgcgca ggttcttggg
     6301 gttggtcagg ttattcaagg tggcttgcga gagcagcacg aagtcccccg cgttgtcttt
     6361 tttaagttgc gtctcaatgg ccttaggcag gaagaaggtg tccgggccgg ttttatggat
     6421 gaagatgccg ctgttcatct ggtccgaagt cccgtaccag cggtattcaa catgcagccg
     6481 gtagtcggca taactctgct gcgtcatcag gtagccgaac ggcttccctg tgcaacggat
     6541 cacgccgtcc gccacggacc acgtggcgtc cgggcaggcg cccggttcag cggtgatgac
     6601 cttggtccat ccggagagat cgcggccgtt gaacagcgtg attttccggt cgggccgaat
     6661 gggctccgat ttgacaagca tttcgctgcg gacggtgacg gcacaggtta aggcgagggc
     6721 gagcagaaat aacttttgca tggttccggt ctcctaattg tgaggtcgtg tggtgtgcag
     6781 tgtcccatct tcccgtgacg ctgtcaacgg ggtatgacgg ttcccgggtg attagcccgc
     6841 cgcccgcgct tcgcacggga atgcaaatga ggataacttt gggtgtaagt tataggcaag
     6901 cccgccgtgc gccggagccg cgagaaaact ttgggatgcg tttttatgac gggatgacag
     6961 gattttcagg attaaaaaaa tcacgttaat cctgtaatcc tgtcaattta taggcaagga
     7021 gtttcgtaaa gggcacgcaa tttcgatacc ctgcatcggg ccggggtcag gaccaggcgc
     7081 gacggcggat ttcgcgcggc ggctggccct gcatgcgccg aacgcagcgc gaaaagtgga
     7141 acgggttctt gaacccgcac tggtcggcga tctctgtcac cgacaggccg gtctccgcca
     7201 gcaacgccaa gcctttttca gtacggagcc gccacaggtg gcgtgccggt gtggtgccga
     7261 gtgccctgcg gaacttgtag atcagcgcgt tttccgagca gcgcgccgca cgctgcgcgc
     7321 caacaaaaca atcggattct cccagatgct cttccatgta acgcagcgcc cgggtcacgc
     7381 aggcgtcctc acggatcatc agggtatcgt ggtctgcgag attcaggaac tcccaaaaga
     7441 gcgtgagcgc caaagtgtca acaacttgca gcgaggtcgg cgattggacc gtgcgcaaca
     7501 ggaaagcggc ggaaatcaga cggttgaaac attccgaggg gagatgcccc tcatctggcg
     7561 tcgcctcaag ggcatggcgg agcgccgggg gcaggtaacc gggcgtcacg gaacaccaga
     7621 agtggtgcgt cggggtctgg ggatcgaaca ggaagtgttc gcggcgccgg ggtttgaaca
     7681 gatagacgtg cccgacgcgc aactcccgcg gttgcccgtc aagccgaacg cagcaggacc
     7741 ccgagtaaag cagcgccagt tcgaaatcac gttggacgcg tgggccgcaa tatccgccgg
     7801 gctggtaacg aacctggccg aacgtgacct gttctgagtg gatactgcgc tgatcccact
     7861 cgatgttgac accatcatag ttagggagaa gactttccat gagcggatcg aacatagcag
     7921 agaagaacgg acttttgcaa gacgttttgt gacggcggat atgttatttt ggattagggg
     7981 tattcactaa gcttgcgaaa aggggtatat taaaaaggaa atgtgacatt gggtatgttc
     8041 caatggaaga gcgggaaagc cgggttcacc cggcttcgca aggctgatcg gaaaagtgag
     8101 tcatcagaaa aaggaggctg agaatgagta tgtttatggg gaaaacaccc tgttcaggtt
     8161 gtcgtctgga acgtcgcgat ttcttggcgg ccgcaggctg tacggcctgt gccgcaacgt
     8221 tgggacgtgt gatggcggcc gctccggcga gagcggcgga aaaggcgccc gatttgcgta
     8281 tccgcatcct gtttgccctc catgcgcctg tgcagccaaa accggactgg ccgaacatcg
     8341 gatttaattt ccagccggtt atggaccaat ttgtggaggg gttgcggacc cattgcccgg
     8401 ggttcgattt cgtcacgtcg atggcggcca atgaggagga ggcgaaagcg attgtcgccg
     8461 cggatcaagg cagccggatc gacgggtata tcgtctatca gttgaactgc tggaacaggg
     8521 tggtgcagcc ggccatagcc acgggcaagc cgaccttgtt cgtcgatttc gcctacggcg
     8581 gcagcggcgg aatgctggtg tacacctccg gttttctgat ctacaaggtg ccgaatgtcg
     8641 gttttgtctc ttcctcccgg ttcgaggatg tcgcggcggc ggtccaatgt ttcgcgcagg
     8701 tcaaacgtgg cggcacggat ttcgcgacgt tgactgcggc gtgccgacgg aagttgacgc
     8761 cgcaggcggg cgacctcagt tgtgtgaatg atccggtcga ggtgctttcg gccaaggatt
     8821 gcctggcgaa gatgcgggaa gccaaactgc tggtggtgac aaacaagtcg gtcggaccct
     8881 atccggcgat caaagagcaa atggggatcg aggtcgttca gataccgttt gaggaactga
     8941 acacggcatg gaaggcagcc gacaaggatc aggcgcgcgc cgttgccgac cgctggcaga
     9001 aggacgcgcg cgcgattatc ggcgtttcgc gggagacgct tgaaaactcg gcggcgatgt
     9061 atctgggtca aaaggccctg ctgaaaaagc atggggcgaa cgggctcacc gtcaattgcc
     9121 tcggcggctt ttacggcggc cacatccacg cctacccctg catgggcttt cacgagttgg
     9181 tcaatgaggg gttgatcgga gggtgcgagg ccgacgtgcg ctcggcggtt tccatgatcg
     9241 ccatgaccac gttgacccag gggcgccccg gtttcatttc cgatccggtg atcgacacgt
     9301 caaagcggca gatcatctac gcccattgtg tcgcctcgaa caaacccttc gggccgcagg
     9361 ggccttccaa cccctttgag atcatgacgc attcggagga ccgcaaaggc gcggcggtgc
     9421 ggtcgctgtt cccggtgggg tacatgacca cgacggtcga gttcgatgcg aaccggaaaa
     9481 cggtcctttt tcatcaggcc aaggcggtgg ccaatgtgga cgatgaccgt gcctgccgca
     9541 cgaaactggc cgccgagccg gtcggcgaca tggagaaact gttcacccat tggaaccgat
     9601 ggggatggca ccgcgtgacc gttttcggcg atctcaaaga accggttttt gcgctggccg
     9661 acgccatggg ctggaaagtc gtgcaggaag cgtgacggtg cttttatgaa ca
//
LOCUS       DAOSLE010000102         9669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6799_length_9669_cov_8.522155, whole genome shotgun sequence.
ACCESSION   DAOSLE010000102 DAOSLE010000000
VERSION     DAOSLE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6799_length_9669_cov_8.522155"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="PK576_07620"
     CDS             complement(<1..1354)
                     /locus_tag="PK576_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE61498.1"
                     /translation="MKQNMPSTTPSRDLGNLCAALQACDRLEPYARYFADAVCRLAPV
                     GLGGAPAAWLAAALTAQNTVVNKHVCLALDPGLRLTDLLPPESIATDAPDAEDLFSAQ
                     LDDLAETLRGKECAFAVTVDPPSDVVPHTPLVLEQNRLYLQRYWLAENTLASQLNARA
                     TETPQSAPDRVEPQTLTRLTLDDAQRAAIRQALTHRLCVITGGPGTGKTTLVSVVLAA
                     LLRETPRRIIRLCAPTGKAQARLKEALDGEINRHLRLEHDEDLRQRLLALETSTLHRL
                     LRARPPLGRFAFHASNPLAADTLIVDEVSMIDLPLMVSLLTAIPSACQVILLGDSDQL
                     AAVEIGAAMGEMCAAWEGTRAVARLTRSHRFDPERGIGRLKDAVCAGDADRAWEILKT
                     GDDALGHAASPAGYANLETALASHLRDHPVRAYLAAPTPAESFARFDAFRILCATRHG
                     P"
     gene            1669..2889
                     /locus_tag="PK576_07625"
     CDS             1669..2889
                     /locus_tag="PK576_07625"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61499.1"
                     /translation="MGIIGCGNQSEHDLKQWFAMDDVQVVSVCDVNRGSHGYRSPQQF
                     LGREPVRDLVNQAYAQRAASGTFKGCGMVADFRELLARRDIDTVAIITPDHWHAIQTI
                     QAAAAGKDIYCQKPLGLTVQDGQEMIRAVRKHNRVLQTGSQWRSTPSLRRFYDLVRGG
                     AVGTPRKAVTYVARNNFTGPGPGWQPMPVPQGFDYDMWLGPAPDAPYHKDRCFYRFRF
                     ISAYSGGQTTNFGHHAIGVAMWALGLDEIGPETVWDMGAEWPPAGDLFDTATKVHFGC
                     RYPGGFELECVSSPKSFGVRVEGDKGWIETNGFKIQSSSPDLVWNPTNVRAEAEFFNH
                     YRNFIDCVKSRGTPVESVVIGHRISTICHIGNIAMKLSRKIRWDAQAEQFVGDDEANA
                     MLRSRHRAPWSFSV"
     gene            3081..5063
                     /locus_tag="PK576_07630"
     CDS             3081..5063
                     /locus_tag="PK576_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOE61500.1"
                     /translation="MKKIMLAAATVVSIVCGAETCAADHPITENERPGREATEWSMSN
                     NYNCRDIGKLPRVMLIGDSITAQYQGAVGRELKGKAYYSYWASSYCVASAMHRTLLKA
                     YLTDLKYDVIHFNNGLHSLSTDPKVWARALKAALLLCRELQPQAKLIWCNSTTVEGEG
                     MNEKVIRLNHAALEVCKELGIDLIDDQFSLVAAQPDAHSDAYHLKGPVKELQAKQVAE
                     ICLRALKPAVSVVDSGAKGDGVHDDTPAFQRAINAAAADKGTVEVPRGVYFLKALEMK
                     SGVTLHLAAGAHLKGSRRIEDYGEGKDLYGKRLGMIRFLGIRDAAVIGELGSLIDGSN
                     SSSPTEFEARGVHLFHVTGSTNLLFRGYTVVDAGNYAHWFRHCADVRFDHVRIEGGHD
                     GVHFWNSSRVTVEDCEFNTGDDCVAGLDYRDLVVRRCKLNTPCQCLRLGGRRILVEDC
                     ELTGPGKHPHRCTLPRQAVLDGLEPAGEGRRYNLAAFLYYCEKDGDVKEDSGEIVVRN
                     CRVRNVQRFFHYNFDGSEIWAQGRPLRDIRFENVTAENVDFPLVAISTLECPTRLTLE
                     NCSIAFTKPVPEFIRTAAFGKLTLKNVRLSGVEGPLVRAWGRPPDVVAENVTGASGIK
                     EGAASEKIDAHPILGFLMDYPEHEMSPLNPKNLKRK"
     gene            complement(5107..5472)
                     /locus_tag="PK576_07635"
     CDS             complement(5107..5472)
                     /locus_tag="PK576_07635"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOE61501.1"
                     /translation="MDWYALADEEVRTVYRDLPDDLRTCLARLTVTLDSRPAPSERSE
                     PGDRDGLLGLFTGPGFAESEDSDMPMPAAIRLFVENLRDEARNDPLLFRQEVRTTLLH
                     EFGHYLGWDESDLAARGLA"
     gene            complement(5480..6100)
                     /locus_tag="PK576_07640"
     CDS             complement(5480..6100)
                     /locus_tag="PK576_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61502.1"
                     /translation="MTRIIPLSLCLLLAASGCAVFGKRANDTGSIPCHYRFKMLSRSE
                     DQMRVEAAIQSVAVGTVTRAGTAAYPEYRFRVARLADLDTLHPKLMFVESASIWSRDR
                     KQALNLKAAEVGAVFDSTDVSASAATTVTLSVKPGSRLYYKMPGGTETEITSRVDRNG
                     QVRFPVTLREGQKFVYVRAVKDNVTRYIRVNLFTNELRDLTKREYR"
     gene            complement(6097..6876)
                     /locus_tag="PK576_07645"
     CDS             complement(6097..6876)
                     /locus_tag="PK576_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE61503.1"
                     /translation="MNRTVLITGAARRIGEALARELARAGWNVVLHAHHSIRAADALR
                     DELRAQGVQAWRVRGDLQPPGGADAVFDAALAEAGGIDAVVNNAACFERQSLADATPE
                     AFDALWRLNTLAPIRLTQRLAAHTSARQTAGCVVNLLDQRIALPHAGCLPYLLSKQAL
                     ATFTTCAALEWAPSGLRINAVAPGAVLTPVTEADREPAGGFPLGRRPTVRHIAEAVRF
                     LLEAESVTGQILYTDGGQHLMGQAHLATPPDFHKPESGVHA"
     gene            6964..7860
                     /locus_tag="PK576_07650"
     CDS             6964..7860
                     /locus_tag="PK576_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61504.1"
                     /translation="MNLRVAERDFKYYIFDWDNNILHMPTRIHLEKRMPDGSWQPHSV
                     STAAFAVLRKDPERYRPPGGVWPRAFVEFQDAHGEGESRFLRDTRDALRRVLSGEIKP
                     GPSFETLRETLREGRLFAIVTARGHEPDSIRKAVQLFIDMVLTHEERQEMMANLRGYR
                     ACFDGLTTFGTDEEELEYYLSLNRYHAVTHPGFLQWMSAEEGDAGEADTASAKQFAIR
                     DFVEHVFRLLSRTGAESLRRPISVGFSDDDPENVAAVERYIQEELSRRFPAIKFCVYD
                     TSDPALKDGRKVTVAGQLDFGF"
     gene            8225..9421
                     /locus_tag="PK576_07655"
     CDS             8225..9421
                     /locus_tag="PK576_07655"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOE61505.1"
                     /translation="MKPQQTVLFLGDGMADEPIGELNGRTPLQVARIPNMDTIARNGR
                     SGTMLTLPAGFPTSSDVANMSVLGCDLATELCGRGPLEAASQGIALGPDDIAFRMNLV
                     TVDAEGILRDFSGGHIAQADAVESIRLLNEQLGGEAVRFYPGVSYRNLLILSGPQFSA
                     NISYDKPDDQHGQPVAKHLPRTKSEEGRFTAALLRDLIARAAEVLQGRTANGIWPWSG
                     GKAGAIHNFCERYGVRAAVISAVDVINGLGRCLGMDVLPVPGATGYIDTNYEGKADAA
                     LKALADGYDFVYVHVEAIDEVSHARDLQLKIKTIEDFDRRLVGRVMAGAGDRVAYCVL
                     PDHPVPVATGKHTRDPVPVAVWQPGVKPDAVAVFDEVAARRGALKHLKGADLMNLLLR
                     ATDPQS"
BASE COUNT         1756 a         2793 c         3297 g         1823 t
ORIGIN      
        1 acggaccgtg gcgcgtggcg cacaggatgc ggaaggcgtc gaaccgcgcg aaagactcgg
       61 cgggtgtggg ggcggcgaga taggcgcgga cgggatggtc gcggaggtgg gaggccaaag
      121 cggtttcgag gttcgcatat ccggcgggcg aggcggcgtg cccgagcgcg tcgtcacccg
      181 tcttgagaat ctcccaggcg cgatcggcgt cgcccgcgca caccgcatcc ttgagacggc
      241 cgatgccgcg ttcagggtcg aaacggtgcg accgggtcag gcgcgcgacc gcgcgtgtgc
      301 cttcccaggc cgcgcacatc tcacccatcg ccgcaccgat ctccactgcg gcgagctggt
      361 cgctgtcgcc cagcagaatg acttggcacg cggacggaat ggcggtcagc aggctgacca
      421 tcagcggcag gtcgatcatc gagacctcat ccacgatcag cgtgtcagcg gccagcgggt
      481 tggacgcatg gaacgcgaac cggccgagcg gggggcgcgc gcgcagcagg cggtgaaggg
      541 tggaggtctc cagtgcgagc agccgttgcc ggagatcctc gtcatgctca agccgcagat
      601 ggcggttgat ctccccgtcc aaggcctcct tgagacgcgc ctgggctttg ccggtcggcg
      661 cacagagccg gatgatccgg cggggggttt cccgtagcag ggctgcgagc accacactga
      721 cgagggtggt cttgccggtt ccgggaccgc cggtgatcac gcaaaggcga tgggtcagag
      781 cctgccggat ggctgcgcgc tgggcatcgt ccagagtgag gcgcgtgagg gtttgcggct
      841 ccacgcggtc gggagcggac tgcggggttt cggtcgcgcg cgcgttgagt tgggatgcca
      901 gagtgttttc ggccagccag tagcgttgga gatagaggcg gttctgctca agtacgagcg
      961 gggtatgggg cacgacgtcg gacggcggat cgaccgtgac cgcgaaggca cactccttgc
     1021 cgcggagcgt ctcggcgagg tcgtccagtt gcgcggagaa gaggtcctcg gcgtcgggcg
     1081 catccgttgc gatggattcc gggggcaaaa gatcggtcag ccgcagaccg ggatcgaggg
     1141 ccaggcagac atgtttgttc accacggtgt tctgtgcggt cagcgcggcg gccagccagg
     1201 cggccggggc accgccgaga ccgaccggcg caagccgaca gaccgcgtcg gcgaagtacc
     1261 gtgcatacgg ttcaagccgg tcgcaggctt gcagcgctgc gcacaggttt ccgagatcgc
     1321 gggatggggt ggtggagggc atgttttgtt tcatggggcc ggggttctgg cccgcgttcg
     1381 gaaccatagt cgtgtttctg ccggtgccgg tcaagtgcgt tttgccggct tgacaggttt
     1441 ggggtaattg gaatatgttg cttttgtagg ttatggtatt cctattgaaa caggtcataa
     1501 aacgggaaac gggaggaggg ctgaagaatg tcgaagaggc tttccgatgc gccgggtgtc
     1561 aggtccagac gggggttttt acgcacagcc gcttcagggg ccgtggggtt tccattgttg
     1621 atccgatccg agacctttgg gaagaacgcg ccgtcgaacc ggatcacgat ggggatcatc
     1681 ggttgtggga accagtccga gcatgatctc aagcagtggt tcgccatgga tgacgtgcag
     1741 gtggtgtcgg tctgcgatgt caaccgcgga agtcacggct accggtcgcc gcagcagttc
     1801 ctcgggcggg agccggtgcg ggacctcgtc aatcaggcct acgcgcaacg ggcggcatcc
     1861 ggcacgttca aggggtgcgg catggtcgcg gatttccgcg agcttctcgc gcgtcgggac
     1921 atcgacaccg tggcgatcat cacacccgac cactggcacg cgatccagac gattcaggcc
     1981 gcggccgcgg gcaaggatat ctattgccag aaaccgctcg ggttgacggt tcaggatgga
     2041 caggagatga tccgggccgt gcgcaagcac aaccgcgttt tacagaccgg gagccagtgg
     2101 cgttccacac cctccctccg gcggttttac gatctggtgc gcggcggcgc ggtcggcacg
     2161 ccccggaagg cggtgaccta cgttgcccgc aacaatttca cagggccggg gccggggtgg
     2221 caaccgatgc ctgttccgca gggatttgat tatgacatgt ggctggggcc cgcgcctgat
     2281 gccccatacc acaaggaccg ctgtttctac cgtttccgtt ttatcagcgc ctactccggc
     2341 gggcagacca cgaatttcgg ccatcacgcg atcggtgtgg cgatgtgggc gctgggactg
     2401 gacgagatcg ggccggaaac ggtgtgggat atgggtgccg aatggccgcc cgcaggggat
     2461 ctgttcgaca ccgccaccaa agttcatttc ggatgccggt atcccggcgg attcgaactt
     2521 gaatgcgttt cgtcgccgaa atcgttcggt gtgcgcgtcg agggcgacaa gggctggatc
     2581 gagaccaacg ggttcaagat ccagtcgtca tccccggatc tcgtctggaa tccgacgaac
     2641 gtgcgggcag aggccgagtt tttcaatcac taccggaact tcatcgattg cgtgaagtcg
     2701 cgcggcacgc cggtggagtc cgtcgtgatc gggcaccgca tctcgacgat atgtcacatc
     2761 ggcaatatcg cgatgaagct cagccgcaag atccgttggg atgcgcaggc cgaacagttc
     2821 gtgggggacg acgaggcgaa cgcgatgctg cggagccgcc atcgcgcgcc gtggtcgttt
     2881 tcggtatgag gcacggccct cagacgggcg cccactcagg tttgctccat aatgtcaacc
     2941 ggttcggtcc gcatggcggt ccatcataac tgggaggttc gccgcgcagg gccttgccgc
     3001 aaaggggcgt ggcccgcttt cgcgggcagg cggcgccttg cggccgcgtt gatgtcggtt
     3061 tttgataagg aaacaggaac atgaaaaaaa ttatgcttgc cgcggcgacc gtggtgtcga
     3121 tcgtttgcgg agcagaaacc tgcgctgccg atcatccgat cacggagaac gagcggcccg
     3181 gccgcgaggc gaccgaatgg tcgatgagca acaattacaa ctgccgcgat atcggcaagc
     3241 tgccgcgagt gatgctgatc ggcgactcga tcactgcgca atatcagggg gcggtgggcc
     3301 gggaactgaa gggcaaggcg tattactcct actgggcgtc gagctactgc gtcgcctcgg
     3361 cgatgcaccg cacgctcctc aaggcgtatc ttaccgatct gaagtacgat gtcatccatt
     3421 tcaataacgg gcttcactcg ctttcgaccg atccgaaggt ctgggcgcgg gcgctcaagg
     3481 cggcgctcct cctgtgtcgt gagctccagc cgcaggcgaa gctcatctgg tgcaattcga
     3541 ccacggtgga gggcgagggt atgaacgaga aggtgatccg gctcaaccat gccgcgcttg
     3601 aggtctgcaa agagttgggc attgacctga tcgacgacca gttctcgctt gtcgcggccc
     3661 agccggatgc gcattccgat gcttatcatc tcaaggggcc ggtcaaggaa cttcaggcga
     3721 agcaagtcgc agaaatctgt ctccgggcgc tgaaaccggc cgtgtccgtg gttgattccg
     3781 gagcgaaggg ggacggtgtc catgacgata cccccgcttt ccagcgggcg atcaacgccg
     3841 cggccgctga taaggggacg gtcgaggttc cgcgaggcgt gtatttcctc aaggcgctgg
     3901 agatgaagtc cggtgtgacc ctgcatctcg ccgccggtgc gcatctcaag ggatcgcgcc
     3961 ggatcgagga ctacggcgaa gggaaagatc tgtacgggaa gaggctcggc atgatccgtt
     4021 ttctggggat tcgcgacgct gcggtgatcg gcgagctggg ctcattgatc gacggttcca
     4081 attcgagttc gccgacggaa ttcgaggcgc ggggtgtcca tctcttccat gtcacgggga
     4141 gtacgaacct cctcttccgc ggttatactg tcgtcgatgc cggtaactat gcccactggt
     4201 tccgccattg cgcggacgtc cggttcgacc atgtccgcat cgagggcggt catgacggcg
     4261 tccatttctg gaattcaagc cgtgtgacgg tcgaggactg tgaattcaac accggggatg
     4321 actgcgtcgc ggggcttgat taccgcgatc tcgttgtccg gcgctgtaaa ttgaacacgc
     4381 cctgccaatg tctgcggctc ggcggacgcc gcatcctcgt cgaggactgc gagctgaccg
     4441 ggccgggaaa gcatccgcac cgttgcacgc tgccgcgtca ggctgtgctg gatgggcttg
     4501 aacccgcagg cgagggtcgg cgttacaatc tcgccgcgtt cctctactac tgcgagaagg
     4561 atggggatgt aaaggaggat tccggagaga tcgttgtgcg taattgccgc gtgcgcaatg
     4621 tccagcggtt cttccattac aatttcgacg gcagcgagat ctgggcgcag gggcgtccct
     4681 tgcgcgacat ccgcttcgaa aacgtgactg ccgagaacgt cgacttcccg ctggtcgcca
     4741 tctcgactct ggagtgcccg acccgcttga cgctcgagaa ctgttcgatc gcctttacga
     4801 aaccggtgcc ggagttcatc cggacggccg cgttcgggaa gctgacgctg aagaacgtga
     4861 ggctctccgg agtcgagggc ccgctggtgc gcgcctgggg aaggccgccg gatgttgtcg
     4921 ctgagaacgt gaccggcgca tcggggatca aggagggggc ggcgtccgag aaaatcgacg
     4981 cgcatccgat cctcggtttc ctgatggact atcccgaaca cgagatgagt ccgctcaacc
     5041 cgaagaatct gaaaaggaaa tgagcggctg cggcaaggaa ggcgtccatc gcctctgtct
     5101 tctgtctcag gcgagtccgc gtgccgcgag atcggattcg tcccacccga gataatggcc
     5161 gaactcgtgc agcagggtgg tgcggacctc ctgccggaag agcaggggat cgttccgggc
     5221 ctcgtcacgc agattctcga caaagagacg gatcgcggct ggcatcggca tgtcgctgtc
     5281 ctcgctctct gcaaagcccg gtccggtgaa gaggccgagc aatccgtcgc ggtcgcccgg
     5341 ctcactcctc tccgacggtg cgggccggga atccagcgtg acggtcagtc gggcgagaca
     5401 cgtgcgcaga tcgtccggca agtcgcggta taccgtgcgc acctcctcat ccgcgagcgc
     5461 gtaccagtcc atgtgcgcct cagcggtatt cccgcttggt gagatcgcgg agttcattgg
     5521 tgaacaagtt gacgcggatg tatcgggtca cgttatcctt gacggcgcgg acgtaaacga
     5581 atttctggcc ctcgcggagt gtgaccggga accggacttg tccgttacgg tccacccgcg
     5641 acgtgatttc ggtctcggtc ccgccgggca ttttgtagta gaggcgcgaa cccggcttca
     5701 ccgacagcgt gacggttgtg gccgcgctgg cggaaacgtc ggttgaatcg aaaaccgccc
     5761 cgacctccgc ggccttcagg ttcagggcct gcttgcggtc gcgggaccat atcgaagcgg
     5821 actcgacgaa catcagtttc gggtggagcg tgtcgagatc cgcaaggcgt gcgacacgga
     5881 aacggtactc gggataggct gcggttcctg cgcgggtcac ggtgccgacc gccacggact
     5941 ggatcgcggc ctccacgcgc atctgatcct cggagcggga gagcatcttg aaccggtagt
     6001 ggcaggggat gctccccgtg tcgttcgcgc gtttgccgaa cacggcgcag ccggacgcgg
     6061 cgagcagaag gcaaagagag agcgggatga tacgtgtcat gcgtggactc ctgattcggg
     6121 tttgtggaaa tcgggcgggg tagcgagatg ggcttggccc atcaggtgct ggccaccgtc
     6181 cgtatacagg atctggccgg tcacggattc ggcctccagc agaaaccgca cggcttctgc
     6241 gatgtgccgc acggttggcc gccgtccgag cgggaacccg cctgcgggtt cccggtccgc
     6301 ttccgtgacg ggcgtcagca ctgcgccggg cgccacggca ttgatccgca gaccggatgg
     6361 tgcccattcg agcgccgcgc aggttgtgaa ggtggcgagg gcctgtttgg agagcaggta
     6421 ggggaggcag cccgcatggg ggagggcgat ccgctggtcc agcaggttga ccacgcatcc
     6481 ggcggtttgc cgtgcggatg tgtgggcggc cagccgttgg gtcagccgga tcggggccag
     6541 ggtgttgagc cgccacagcg cgtcaaacgc ctcgggggtg gcgtccgcga gggattggcg
     6601 ctcgaaacac gcggcgttgt tcaccacggc atcgatgccg ccggcctcgg cgagggccgc
     6661 gtcaaacacg gcatccgccc cgccgggggg ctgaaggtcg ccccgcacac gccacgcttg
     6721 gacaccttgc gcacggagtt cgtcgcgcag ggcgtctgcc gcacggatcg agtggtgggc
     6781 gtgaagcacc acgttccagc ccgcgcgggc gagttcacgc gcgagggctt ctccgatccg
     6841 gcgtgcggcg ccggtgatga gcacggtgcg gttcatggta atcgttcgga acgaaaggtt
     6901 gcttattgac aagcgataag ccgcatcata ccttaacccg caatgcgacg tcatgcacaa
     6961 aacgtgaatc tgagggtcgc cgagcgcgat ttcaagtatt acattttcga ttgggacaac
     7021 aacatcctgc acatgcccac gcgcatccat ctggagaagc gcatgccgga cggctcatgg
     7081 cagccgcaca gcgtttcgac agcggcgttt gccgtgctgc ggaaggatcc ggagcgctac
     7141 cgtccgcccg gaggggtgtg gccgcgcgcg tttgtggagt ttcaggatgc gcacggcgaa
     7201 ggcgaaagcc ggttcttgcg cgacacgcgg gatgcgttgc ggcgcgttct ttccggagag
     7261 atcaagcccg ggccgagttt cgagacgttg cgggagacgt tgcgggaggg gcgccttttc
     7321 gcgatcgtga ccgcacgcgg ccatgagcct gattcgatcc gcaaagcggt acagttgttc
     7381 atcgacatgg tgctgacaca cgaagagcgg caagagatga tggcgaacct gcgcgggtac
     7441 cgcgcctgtt tcgatgggct gaccactttc ggtacggatg aggaggaatt ggaatactac
     7501 ctttccctga accggtatca tgcggtcacc catcccgggt tcctgcaatg gatgtctgct
     7561 gaagaggggg acgccgggga agccgacacc gcgagcgcca agcagttcgc gatccgcgat
     7621 tttgtcgaac atgtgttccg gttgttgtcg cgtacgggcg ccgaatccct gcgtcgtccg
     7681 atcagcgtgg ggttttccga tgacgacccg gagaacgtgg cggcggttga acggtacatt
     7741 caggaggagt tgtcgcgccg ctttcccgcg atcaaattct gcgtgtacga cacttcggat
     7801 ccggcactga aagacgggcg gaaggtgacg gtcgccggac agttggattt cgggttctga
     7861 ccgcgctccc gactgggctt catgcaaaac acgctccccg cccgcaacga cggatcagtc
     7921 agatcagtcg ctccgccaaa caatccaaac gtcgcaggtt gaagtacgcg aaaacgggaa
     7981 cgaggacggc gaagcgatcc cgatagggat aaaccagaaa cctgaagtcc ggaaccagaa
     8041 accgagttgc gggcgagggc gcccgcgctc ccgacttttc cccatcttga tttcatgaag
     8101 ggtcatatgt ttcatggtac ggatgtttac aggcctttac ccccaaaagg atgcaactcc
     8161 gctcgtgtca cgggtgaccg ctgattgaca cagccgccgc agtctgcgac actgtacacc
     8221 catcatgaaa ccccaacaga ccgttctttt tcttggcgac ggcatggccg acgaaccgat
     8281 cggcgaactg aatgggcgta ccccgcttca agtcgcccgc atcccgaaca tggacactat
     8341 tgcccggaac gggcgcagcg gcacgatgct caccctgccc gcaggattcc cgacctccag
     8401 cgatgtggcc aacatgtcgg tgctcgggtg cgacctcgcc acggagttgt gcggccgcgg
     8461 accgctcgag gccgccagcc agggtattgc gctcggaccc gatgacatcg cctttcgcat
     8521 gaaccttgtc accgtggatg cggagggcat cctgcgcgat ttctccggcg gacacatcgc
     8581 tcaggcggat gcggtcgagt ccatccgatt gctcaacgag cagttgggcg gtgaggcggt
     8641 ccgtttttat cccggtgtca gctatcgtaa tctgttgatc ctttccggtc cgcagttcag
     8701 cgccaacatc tcgtatgaca aacctgatga ccagcacggg caaccggtcg ccaagcacct
     8761 gccgcgcacc aagtccgaag aagggcgatt cacggccgcg ctgttgcgcg acctgatcgc
     8821 ccgcgcggcc gaagtgcttc agggccgtac ggcgaatggc atctggccgt ggagcggcgg
     8881 caaggccggt gcgatccata atttctgcga acggtacggg gtccgcgccg cggtgatctc
     8941 tgccgtggat gtgatcaacg ggctcggacg ctgtcttgga atggatgtgc tccccgttcc
     9001 cggtgcgacg gggtatatcg acacgaacta tgagggcaag gccgatgccg cgctcaaggc
     9061 gctcgcggac gggtatgatt tcgtctatgt gcatgtcgag gccatcgacg aggtttcgca
     9121 cgcgcgcgac ctccaactga aaatcaagac gatcgaggat ttcgaccggc ggctcgtggg
     9181 gcgggtcatg gccggcgcgg gtgaccgggt cgcgtactgc gtgttgccgg atcatccggt
     9241 gccggtcgcg accggaaaac atacgcgcga cccggtgccg gtcgcggtgt ggcagcccgg
     9301 cgtgaagccg gacgcggttg cggttttcga cgaggttgcc gcgcggcgtg gcgcgttgaa
     9361 gcatctgaag ggtgcggacc tcatgaacct gctgttgcgc gcgacggacc cgcagtcctg
     9421 aggggaaagt tgggcaaagc tggcggaaat cctgacgcgt tttatatctg tgtaatccta
     9481 aaaagagcag ttgaaccttc aactctcttt ggtttcaagg tttctgggtt tcagggtttc
     9541 tggtttcatg gtttcatcca aatcggggtc gggatcggga tcgctatcgg tatcgggatc
     9601 gctatcggta tcggtatcgg gaacgtcgac ccgcgacccg cgacattcga cccgcgacga
     9661 tcgacaggg
//
LOCUS       DAOSLE010000103         9535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6904_length_9535_cov_7.849789, whole genome shotgun sequence.
ACCESSION   DAOSLE010000103 DAOSLE010000000
VERSION     DAOSLE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6904_length_9535_cov_7.849789"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK576_07660"
     CDS             complement(<1..241)
                     /locus_tag="PK576_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE61506.1"
                     /translation="MTDAPARSAANRVVPRQYLFPFVLTTSLFALWGFVNDITNPMVA
                     AFKAVFIEISNTQSSLVQLAFYGGYATMAIPAALFI"
     gene            complement(412..>658)
                     /locus_tag="PK576_07665"
     CDS             complement(412..>658)
                     /locus_tag="PK576_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61507.1"
                     /translation="VGLSNFTVMLPPRRADGSPIRSALASAFLTRAMPLGLDMIIGSV
                     KTPYTLLPEGHPALQCFDECLAADGFDVLERVQAFCE"
     assembly_gap    659..668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<669..1282)
                     /locus_tag="PK576_07670"
     CDS             complement(<669..1282)
                     /locus_tag="PK576_07670"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOE61508.1"
                     /translation="MSLPGLTIIGESINDSIPSTHALFEARDLDAIARLAQTQAGLGA
                     AYIDVNVGPRAPDYMAEVVRAIQSATTAPLSLDSPDPLLARAALEAYDPAHGVPILNS
                     ISPLRMEMFELIALKPFRPILLASETVRDGMEIPCRTAEETVAAAALLLDAAHRHGIA
                     NHDCILDPGIAPLGTDTENNIHRLMGALRIIRDDPAFTGVHLSVG"
     gene            complement(1306..2217)
                     /locus_tag="PK576_07675"
     CDS             complement(1306..2217)
                     /locus_tag="PK576_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61509.1"
                     /translation="MNNLIPRVPQNPCRSRRPRGARRCLALLLLSAGLSAGAADRLPA
                     HLYPQNDAFRKEMAELRARGITLTDYHIHIRGGMTPQKAAERQEQSGIRSAILENHGR
                     EWPICDNARLAAFLDDCSRVQAHGKRLPVGIQVNDRDWHRTLDPALFARLDFVLADTM
                     IMGVTADGKPQRLWLPDVTIENPEAWMKEYVAHNLRILDEPISILANPTYLPPCISNQ
                     YDRLWTEARMRAVIAKAVSRGVALEIQAESPYPSPVFITLAKSMGARFSFGSNNKTDQ
                     TKNPARWFETLRKYDLRPDQLWTPQRP"
     gene            complement(2255..2686)
                     /locus_tag="PK576_07680"
     CDS             complement(2255..2686)
                     /locus_tag="PK576_07680"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE61510.1"
                     /translation="MTEPRRITLPYRVPYADTDQMRVVYYANYLTLFERARNELMRDC
                     GYTYRNLEAEGFALPVLEAHVTYHAPATYDDLLEITAWCERFQGVRLTIRCEVRRDGV
                     LLADGYTVHAHVNAATLRPTRPGKNLLTALGLVTAMPEEKG"
     gene            complement(2683..3489)
                     /locus_tag="PK576_07685"
     CDS             complement(2683..3489)
                     /locus_tag="PK576_07685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE61511.1"
                     /translation="MTPNHLLAIASHATRRAGEYVLANLHRRGDVNTLLRTDVKHKLD
                     IEAQEIATETILAAFPDHAILGEETCDTPLPESDYVWVVDPIDGTINFFHGLPLWCCS
                     VAVRHRGQTVAGSVFLPELRQLYEATCDGPAYCNGVPLKVSSTDRLEHAMLATGADKW
                     DLGTYAFTFMRRVAEIAQRPRILGAAAVDLCLVAAGKLDGYFESGIYHWDMAAAGLIV
                     ERAGGTCEILREFPGYRIAFLATNGVLHAACRDALLPLLASSQPKTGAQP"
     gene            complement(3642..4838)
                     /locus_tag="PK576_07690"
     CDS             complement(3642..4838)
                     /locus_tag="PK576_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE61512.1"
                     /translation="MIRLLPQLVLAVLCAGFPGAAETAPNTFGNHVVARGAFQNARIR
                     FEREQRGTVAFLGGSITEMNGYRPMVCSILTNRFPRTAFTFIQAGISSTCSTTGAFRF
                     GTDVLSQGRVDLLFIEFAVNDDQDAHHDRNACIRGMEGIVRQARRANPEMDIVITFFV
                     NEFILKTLQAGRTPLTIEAHEAVAAHYAIPTIHLAGEVAKRITAGSLTWKQFGGVHPA
                     PLGNALCASMIRELMDRAWSRPLTPESATVPAPMPPTPLDPLNYANGRFLDPAAARFG
                     QGWTLGIPDWQSLPGTKRPRFSAIPMLCATEPGSELTLAFEGTAIGAYLVAGPDAGTV
                     AVRIDGKPFPDVNLDHAYSKGLHYPRTVMFGTGLDAGPHTLSLRIAPTTSSTGHAIRI
                     MRFAAN"
     gene            complement(4849..5361)
                     /locus_tag="PK576_07695"
     CDS             complement(4849..5361)
                     /locus_tag="PK576_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOE61513.1"
                     /translation="MTKRKKTIADPRPDWDTYFMEIARVTAMRANCSRRKVAAVVVSD
                     NRIISTGYNGTPRGVTNCFAGGCARCAGTAPSGSSLEECICVHAEQNAICQAAYYGTR
                     LAGATIYVTISPCLTCAKLIINAGIKEVVYDGDYAFTEQTERLLQEAGVTCRRYVSGA
                     GVCLPASQGE"
     gene            complement(5361..6683)
                     /locus_tag="PK576_07700"
     CDS             complement(5361..6683)
                     /locus_tag="PK576_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61514.1"
                     /translation="MKLTRRTMLKRTALAGMPFLLPSSIRAATTSPNSRPTLGVIGMG
                     IRSRQLLDSFCHDMQVLAICDADTTRREDGCGRVNAYYARFPEKGTGGCVALRDFRDL
                     LARRDIDAVYIGTPDHWHAFQAIAALRAGKDVYCEKPLTHNIHESLALMRVVRETGRV
                     LQTGAQQRSDKTFRVACELVRNGCIGKIERIDCSFGGPARPYNLTAEPLEPGLDWDLW
                     CGPGPLVPYNSVLSPRGVHSHFPRWRGFREFGGGSTADLGAHHLDIIQWALDRDASGP
                     VEARPPAEAKAGFGAELVYADGVTVRHISGGFSIRFYGTEGVIDVGRGTFRLERQGQE
                     IARFLKREDGSSCEAQVLKAERAFLANAKVKLYNSRSHLSDFLACMANRKAPISNVEV
                     GARSAICCHLLNQAYYNREPIRWDPEGLRLTGGNPAWLTREYREPWSV"
     gene            6813..7574
                     /locus_tag="PK576_07705"
     CDS             6813..7574
                     /locus_tag="PK576_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE61515.1"
                     /translation="MMGEVLKNIRVVLVGTLYHGNVGAVCRAMANMGIGDLVLAAPQL
                     CDDPAEAERMAVHATDVLKRRRVTASLAEAVADCAFTVGTTARGGLYRQHVKTPREWA
                     PELLRLAEQGRVALVFGREDKGLLNDEIGLCTHLIRIPVALAYTSINLAQAVMICCYE
                     LFAAVGIYEPPVEKSAPAVAARKTKMLELWRAMLLQIGFMKEDKADHMMQGVQRIFSR
                     GVYSDDDVAIMMGVARQADWASRNAPVSRSGKTET"
     gene            complement(7614..8936)
                     /gene="rmuC"
                     /locus_tag="PK576_07710"
     CDS             complement(7614..8936)
                     /gene="rmuC"
                     /locus_tag="PK576_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOE61516.1"
                     /translation="MNLFILTFLLLALVLVLLVVLIVRTGRDAPDRAALARLQTRDAD
                     YQEATRLLREQSERLESLQVENARLTAELEHERRNAAEKLRLLQDAETRLKTEFENLA
                     NRIFEDKGKAFTEQNRERLTGLLQPLKEQLDGFRKRVDEVHKDGTEQSARLLEQVRQL
                     QELSNRVSDEANQLARAIKGDAKRQGDWGELIVERLFEASGLEDGREYEAQPLLRTED
                     GAARRPDFVVHLPQGKSVIVDSKVSLTAYERYCNAETEEDRRGALEAHVQSIRRHLEE
                     LRGKNYTALLGNRTLDFVLMCIPIESAYQTALQADPKLLYDLAQTRVVITGPSTLMIT
                     LKLIAQIWRREKENRHAEQIADRAGRLYDQVTLIVEAMTAAKKNLAETTNCFDLALAR
                     LQTGRGNLVGRIEVLRKLGAKVSKALPPQVIEAALATEPAENDEPAQP"
     gene            complement(9026..>9535)
                     /locus_tag="PK576_07715"
     CDS             complement(9026..>9535)
                     /locus_tag="PK576_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HOE61517.1"
                     /translation="RHGPCGVDAANRYVQTLLGVAPYAHGYPVMVTVNDASRNLFNGD
                     IGLCLRDPQHNGTRVWFPDPERAAAEPHSPPGRALRSFATADLPEHEPVFAMTVHKAQ
                     GSGFDDVLLLLPPYPTPVLTRELLYTGITRARTRCTVWANEPIFKDAVRNRTRRMSGL
                     QNKLARLDG"
BASE COUNT         1685 a         2804 c         3213 g         1823 t
ORIGIN      
        1 ggatgaagag cgcggcgggg atcgccatcg tggcgtagcc gccgtagaag gcaagttgca
       61 ccagcgagct ttgcgtgttg ctgatctcga tgaagacggc tttgaaggca gccaccatcg
      121 ggttggtgat gtcatttacg aatccccaga gggcgaacag cgaggtggtt agcacaaagg
      181 ggaagaggta ttgccgtgga accacacggt ttgccgccga ccgggccggt gcatctgtca
      241 tactgaactc ctgattgaat cggattagaa tgaaaacacc gcatactgta ctggattcgc
      301 tacgctaaag aaatccaatt ttgttcaaag tccgcaattg cccggacagg cggattccca
      361 acggttccgg atcgtgggtc tggcgcgcgc cgcggatttc cggtaatggc ctcattcgca
      421 aaacgcctgc acgcgttcca gcacatcaaa accgtcggcc gcgaggcatt cgtcgaagca
      481 ctgcagggcg ggatggcctt cgggaagcag cgtgtagggc gttttgaccg aaccgatgat
      541 catgtccagc ccgagcggca tggcccgcgt caggaacgcg ctggccagcg cggaccggat
      601 cggcgaaccg tccgcacggc gcgggggcag catgaccgtg aagttactga gacccaccnn
      661 nnnnnnnncc caccgagagg tgcacgcccg tgaacgcggg atcgtcccgg atgatccgca
      721 gtgcgcccat gaggcggtga atgttgtttt cggtgtcggt gccgagcggc gcgatgccgg
      781 ggtcgaggat gcagtcgtgg ttggcgatcc cgtgccggtg cgcggcgtcc agcaggaggg
      841 cggctgcggc gacagtctct tcggcggtac ggcagggaat ctccatgccg tccctcacgg
      901 tttccgaggc gagcaggatc gggcggaagg gcttgagcgc gatcaactcg aacatctcca
      961 tccgcagggg ggagatggag ttcaggatcg gcaccccgtg cgcggggtca taggcctcga
     1021 gcgcggcgcg cgcgaggagc ggatccgggg agtcgagcga taacggcgcg gttgtggcgg
     1081 actggatggc gcgcaccact tcggccatgt agtccggcgc gcgcggaccg acattgacat
     1141 caatgtaggc ggcgcccaga ccggcttgcg tttgcgcgag ccgcgcgatg gcatcgaggt
     1201 cgcgggcttc aaacagcgcg tgggtggagg ggatcgagtc gttgatgctt tcgccgatga
     1261 tcgtaagtcc gggcagggac atgggaactc ctttcgatac gggggttacg ggcgttgggg
     1321 cgtccagagc tggtcgggcc gcaggtcata cttgcgcagg gtctcgaacc agcgtgcggg
     1381 attcttggtt tggtcagtct tgttgttcga gccgaatgag aagcgggcac ccatggattt
     1441 cgccagcgtg atgaaaacgg ggctgggata gggcgattcg gcttggatct ccaacgccac
     1501 gccgcggctg accgctttgg cgattaccgc gcgcatgcgc gcttccgtcc agagtcggtc
     1561 atattggttc gaaatgcagg gcggcaggta ggtggggttg gcaaggatcg agatcggttc
     1621 gtcgagaatg cggaggttgt gcgcgacata ttctttcatc cacgcctcgg ggttctcgat
     1681 cgtcacatcc ggcagccaga ggcgctgcgg tttgccgtcg gcggtcacgc ccatgatcat
     1741 ggtgtcggcg agcacgaaat ccagccgggc gaacagggcc ggatccaggg tccggtgcca
     1801 gtcgcggtca ttcacttgaa tcccgacagg cagccgcttt ccatgggcct gcacacggga
     1861 acagtcatcc agaaacgcgg cgaggcgggc attgtcgcag atcggccatt cccggccgtg
     1921 gttctccaga atcgcgctgc ggatgccgct ttgctcctgg cgttccgccg ccttctgcgg
     1981 cgtcatgccg ccacggatgt gaatatgata gtcggtcagc gtgatgccgc gcgcgcggag
     2041 ttccgccatc tctttgcgaa acgcatcgtt ttgcgggtag aggtgtgcgg gaagccggtc
     2101 tgcggcacct gcggacaggc cggcggacag caacagaagc gcaaggcaac ggcgcgcgcc
     2161 acgcgggcgg cggctgcggc aaggattttg cggaacacgg ggtataaggt tgttcatgtc
     2221 gggactataa cacggggacc gcacgggatg aaagtcatcc tttctcctct ggcatggcgg
     2281 tcacgagtcc gagtgcggtc agcagattct tgcccggccg ggtgggccgc agggtggcgg
     2341 cgttgacatg ggcgtgcacc gtgtacccgt ccgccaaaag cacgccgtcg cgccgcactt
     2401 cgcaccggat ggtcaggcgc accccttgga accgctcgca ccaggcggtg atctccagca
     2461 ggtcgtcata ggtggcgggc gcatgatatg tgacatgggc ttcgagaacc ggcagggcga
     2521 acccctcggc ttccaggttg cggtaggtat agccgcagtc ccgcatcagc tcgttgcgcg
     2581 cgcgctcgaa caaggtcaga taattcgcgt aatagacgac ccgcatctgg tcggtgtcgg
     2641 catacggtac acggtacggg agggtgatgc gacggggttc tgtcacggtt gcgctccggt
     2701 tttgggttga gaggaggcga gtaacggcag gagcgcgtcg cggcatgcgg cgtgcagcac
     2761 gccgttggtt gcgagaaacg cgatgcggta tccggggaac tcgcgcagga tttcacaggt
     2821 gcctccggca cgttcgacga tcagtccggc cgccgccata tcccagtgat aaatgccgga
     2881 ttcgaaatac ccgtccaatt taccggcagc caccagacag agatccactg cggcggcacc
     2941 cagaatccgc ggccgctgcg cgatttcagc gacgcgccgc ataaatgtga aggcataggt
     3001 cccgagatcc catttgtccg caccggtggc gagcatggcg tgttccaaac ggtcagtgga
     3061 cgaaaccttc agcggcacgc cgttgcagta tgcgggcccg tcgcaggtgg cctcgtacag
     3121 ttgccgcagt tccgggagga agaccgagcc cgcgacggtt tgtccccggt ggcgtaccgc
     3181 gaccgagcaa caccacaagg gcaggccgtg gaaaaagttg atggtgccgt cgatggggtc
     3241 gacgacccag acataatcgc tctcgggcag cggggtgtcg cacgtttcct ctccgaggat
     3301 ggcatggtcg ggaaaggcgg caagaatcgt ttctgtggcg atctcctgag cctcgatatc
     3361 cagtttgtgt ttcacatcgg tgcgcagaag cgtgttgaca tcaccgcgcc ggtggaggtt
     3421 ggcgagcaca tattcaccgg cgcggcgcgt ggcgtgggaa gcgatggcga gcagatggtt
     3481 gggggtcatg gcaaaacctt ttgacgggga tccgtcaggg cggatccgga aggccggttg
     3541 cttttccaat gggtcgtgtt cattgtgatc agtctaccac aaaaccggac cggcaaacca
     3601 aaaaaaccaa acgtcgcagg tcgaatgtcg cgggtcgtgc gtcagttcgc agcgaaacgc
     3661 atgatgcgta tcgcgtggcc ggtactggac gtggtcggcg cgatacgcag gctcagcgta
     3721 tgcggtcccg cgtcgagacc ggttccgaac atcaccgtgc gggggtaatg caaccctttg
     3781 ctgtaggcgt gatcaagatt cacgtcaggg aacggcttgc cgtcgatgcg gaccgcgacg
     3841 gtgccggcgt ccggacccgc cacaaggtac gcgccgatcg cggtaccctc aaacgccagg
     3901 gtcaattccg agcccggttc ggtcgcgcac agcatgggga tggcggaaaa acgcggccgt
     3961 ttggtgccgg gcaggctctg ccaatccgga atgccgagag tccagccttg gccgaagcgt
     4021 gctgccgcag gatcgaggaa acggccgttt gcgtagttca gcggatccag cggcgttggc
     4081 ggcattgggg cgggtacggt tgcggattcc ggagtgagcg gacgggacca ggcgcggtcc
     4141 ataagttcgc ggatcatgct ggcgcagagg gcgttgccga gcggtgcggg atgcacgccg
     4201 ccgaactgtt tccaggtgag gcttcccgcc gtgatccgct tcgccacctc gccggccaga
     4261 tggatcgtgg ggatcgcgta atgggccgcc accgcttcat gcgcctcgat cgtgagcggc
     4321 gtccgtccgg cttgcagggt tttgaggatg aactcgttca cgaaaaaggt gatcacgatg
     4381 tccatttcgg gattggcccg ccgcgcttgc cgcacgatcc cctccatgcc gcggatgcag
     4441 gcgtttcggt catgatgggc gtcctgatcg tcattcaccg cgaattcgat aaagagcaga
     4501 tcgacccgtc cctgcgacag cacatcggtt ccgaaacgga aggcgccggt ggtcgaacag
     4561 gtcgaggaga tgcccgcctg aataaaggtg aacgcggtcc gggggaaacg gttggtcagg
     4621 atgctgcaca ccatcgggcg gtagccgttc atctcggtga tggatccgcc gagaaacgcc
     4681 accgttcccc gctgttcgcg ttcgaaacgg atgcgggcgt tttgaaacgc gccccgcgcc
     4741 acgacgtggt tgccgaatgt gttcggagcg gtctcggcag caccgggaaa tcccgcgcac
     4801 agaacggcca ggacgagttg cggaagcagt cggatcatga ggtctccttt attcgccttg
     4861 gctcgcgggc aagcagacgc ctgctccgct cacgtatcga cggcatgtca cgcccgcttc
     4921 ctgtagcaaa cgctcggttt gttcggtgaa agcgtaatcg ccgtcataga cgacctcttt
     4981 gattccggcg ttgatgatca attttgcgca ggtcaggcac gggctgatgg tcacatagat
     5041 cgtcgcgcct gccagccggg tgccgtaata ggccgcctga cagatcgcgt tctgttcggc
     5101 gtggacgcag atgcactctt ccagactgga gccggagggt gccgttcccg cgcatcgcgc
     5161 gcatcccccc gcgaaacagt tggtgactcc ccgcggggtg ccgttgtaac cggtcgagat
     5221 gatccggttg tccgacacca ccacggccgc caccttgcgg cgtgaacagt tggcgcgcat
     5281 ggctgtcacc cgcgcgatct ccatgaaata ggtgtcccag tcgggtcgcg gatccgcgat
     5341 cgtctttttt ctttttgtca tcacacgctc cacggttcgc ggtactcccg cgtcagccag
     5401 gccggattcc cgcccgtcag ccggagcccc tccgggtccc agcggatcgg ttcgcggttg
     5461 tagtacgcct gattgagcag gtgacagcag atggccgaac gcgcgccgac ctccacattc
     5521 gagatcgggg ccttgcggtt cgccatgcag gcgagaaagt ccgagaggtg cgagcggctg
     5581 ttgtagagct tcaccttggc gttcgcgaga aacgcgcgtt cggctttcag cacttgggcc
     5641 tcacaggatg agccgtcctc acgcttcagg aagcgggcga tctcctgtcc ctgccgttcg
     5701 agccggaagg tgccgcgacc gacgtcgatg actccttcgg tcccgtagaa ccggatgctg
     5761 aacccgcccg aaatgtggcg gaccgtcacg ccgtcagcgt aaaccagttc ggcgccgaag
     5821 ccggctttgg cctctgcggg cggtcgggcc tccaccggtc ccgaggcatc ccgatccaat
     5881 gcccattgga tgatgtcgag gtggtgcgcc ccgagatcag ccgtgctgcc gccgccgaat
     5941 tcgcggaatc cacgccagcg cgggaagtgc gaatgcacgc cgcgcggcga gaggacggag
     6001 ttgtagggaa caagcggtcc cgggccgcac cacagatccc aatccagtcc cggttccagc
     6061 ggctcggcgg tgagattgta gggccgcgcg ggtccgccga acgagcagtc gatacgttcg
     6121 attttcccga tgcacccgtt gcgcaccagt tcgcacgcca cccggaaggt cttgtcagaa
     6181 cgctgctggg caccggtttg cagcacgcgg cccgtttccc ggacaacccg catgagggcc
     6241 agcgactcat ggatgttgtg cgtcagcggc ttctcacaat agacatcctt gcccgcccgc
     6301 aacgcggcga tcgcctgaaa ggcgtgccag tggtcgggtg tgccgatata taccgcatcg
     6361 atgtcccgcc gcgcgagcag gtcgcggaag tcgcgtagcg ccacacagcc gcccgttcct
     6421 ttttcgggga aacgggcata atacgcattc acccgtccgc acccgtcctc gcggcgcgtg
     6481 gtgtccgcat cgcagatcgc gagaacctgc atatcgtggc agaacgagtc gagcagttgg
     6541 cggctccgga tgcccatgcc gatcaccccg agagtggggc ggctgttggg cgaggtcgtc
     6601 gccgcccgga tggaagaggg aagaaggaac ggcattccgg caagcgcggt ccgcttcaac
     6661 attgtccgac gagtcaattt cataagtccc ctttcttttg gtgcatatac gatacacgac
     6721 ccgtttcccg tttgtcgagt cgcgacacgg cgcgtttttc gttcgcgacc gggccgctga
     6781 tacggtaagg tacctgaatc gacgggaggc ggatgatggg tgaggtcttg aaaaacattc
     6841 gggtcgttct ggtgggcacg ttgtaccacg gcaacgtggg ggcggtctgc cgcgcaatgg
     6901 cgaacatggg gatcggtgac ctcgttcttg ccgcgccgca gttgtgcgac gatccggcgg
     6961 aggcggaacg catggcggtc cacgcgacgg atgtcctgaa gaggcggcgc gtgacggcct
     7021 cgctggccga ggcggttgcg gactgcgcgt tcacggtggg caccacggca cgtggcggac
     7081 tctaccgtca gcacgtgaag actcctcggg agtgggcgcc cgaactcttg cgcctggcgg
     7141 agcagggccg ggtcgcgctc gtgttcgggc gggaggacaa gggactgctg aatgacgaga
     7201 tcgggttgtg cacgcatttg atccggattc cggtcgcact cgcctacacc tcgatcaatc
     7261 tggcgcaggc ggtgatgatc tgttgttacg agctgtttgc ggcggtcggc atctacgagc
     7321 cgccggttga gaaatcggcc cctgcggtgg ctgcccgcaa aaccaagatg ctggagttgt
     7381 ggcgcgcgat gctgctgcag atcggcttca tgaaggagga taaggctgat cacatgatgc
     7441 agggcgtgca gcgcatcttc tcccgcgggg tttattccga cgatgacgtc gcgattatga
     7501 tgggcgtggc gcgtcaggct gactgggcct cccggaacgc tcccgtgtct cgaagcggca
     7561 agacggaaac ctgatccgcc gagcggcggc gggcccatcc ctctgtgcgc gcctcacggt
     7621 tgcgcgggtt cgtcgttttc tgcgggttcg gttgccagag cggcttcgat gacttgcggc
     7681 ggcaaggcct tgctgacctt ggcgccgagt ttccgcagca cttcgatgcg tccgaccaga
     7741 ttcccccggc cggtttgaag ccgggccagc gcgaggtcga aacagttcgt ggtctcggcc
     7801 agatttttct tcgcggcggt catggcctcg acaatcaggg tgacctggtc gtagaggcgt
     7861 cccgcgcggt cggcgatctg ctcggcgtgg cgattctcct tttcgcgccg ccagatctgc
     7921 gcgatcagtt tgagcgtgat catgagcgtg gacggtccgg tgatcacgac acgggtttgg
     7981 gccagatcgt acagcagctt ggggtctgcc tgcagggcgg tctggtacgc ggactcgatc
     8041 gggatgcaca tcagcacaaa gtccagcgtc cggtttccaa gcagcgcggt gtagttcttg
     8101 ccgcgcaact cctccaaatg acgccgtatt gactgcacat gcgcctccag cgcaccgcgc
     8161 cgatcctctt cggtctcggc attgcagtac cgctcatagg cggtcagcga aactttggag
     8221 tccacgatga ccgattttcc ctgcggcagg tggaccacga aatcgggacg ccgtgccgcg
     8281 ccgtcctcgg tccgcaacag gggttgggcc tcatattcgc gtccgtcctc cagtccggag
     8341 gcctcgaaca gccgttcgac gatcaattca ccccaatccc cctgccgttt ggcgtcgccc
     8401 ttgatggcac gcgccagttg attggcctcg tcgctgaccc tgttgctgag ttcctgaagc
     8461 tggcgcacct gctccagcag gcgggcggat tgctcggttc cgtccttgtg aacctcgtcc
     8521 acacgtttgc ggaacccgtc aagctgctcc ttcagcggct gcagcagacc ggtgaggcgt
     8581 tcgcggttct gttcggtgaa agccttgccc ttgtcctcga aaatgcggtt ggcgagattc
     8641 tcgaactcgg ttttgagccg cgtttcggca tcttgcagca accggagttt ctccgctgcg
     8701 ttgcgacgct cgtgctccaa ctcggcggtc agccgggcat tctccacttg cagcgactcc
     8761 agccgctccg attgttcccg caggagccgg gtcgcctcct gatagtccgc gtcgcgcgtc
     8821 tgcaaccggg ccagcgccgc gcggtcaggc gcgtcgcgtc ctgtgcgcac gatcagcacg
     8881 acgagcaaca ccaaaacgag ggccaacagg aggaaggtaa ggataaacag gttcatggtg
     8941 tcagaaaaag atgatgatcg ggtttcgggg ttactcaacg catgagcgat ctccaaatgc
     9001 tgtgttgacg atacgctgta aacggttatc cgtcaagtcg cgccagcttg ttttggagac
     9061 cggacatgcg ccgggtgcgg ttccgcaccg cgtctttgaa aatcggttcg ttagcccaga
     9121 cggtgcagcg tgtgcgggcg cgggtgatgc ccgtatagag cagttcgcgg gtcagtaccg
     9181 gcgtcggata ggggggcagg agcagcagca cgtcgtcaaa ccccgatccc tgtgctttgt
     9241 gcacggtcat cgcgaacacc ggctcgtgct cgggcagatc ggccgtggcg aaggaacgga
     9301 gcgcgcgtcc gggcggggaa tgcggttcgg ctgcggcccg ttccgggtcg gggaaccaga
     9361 cgcgggtccc gttatgctgc ggatcccgca gacagaggcc gatgtcgcca ttaaagaggt
     9421 tacgggatgc gtcgttgact gtcaccatga ccggatatcc gtgggcgtag ggggcgacgc
     9481 cgagaagcgt ttgcacgtag cggttggcgg cgtccactcc gcacggaccg tggcg
//
LOCUS       DAOSLE010000104         9413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_6996_length_9413_cov_10.340564, whole genome shotgun sequence.
ACCESSION   DAOSLE010000104 DAOSLE010000000
VERSION     DAOSLE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6996_length_9413_cov_10.340564"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..329
                     /locus_tag="PK576_07720"
     tRNA            240..329
                     /locus_tag="PK576_07720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:273..275,aa:Leu,seq:taa)
     gene            complement(370..1251)
                     /gene="rfbA"
                     /locus_tag="PK576_07725"
     CDS             complement(370..1251)
                     /gene="rfbA"
                     /locus_tag="PK576_07725"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOE61518.1"
                     /translation="MARKGIILAGGAGTRLFPVTQVICKQLLPVYDKPMIYYPLSTLM
                     LAGIREVLIISTPEATPVFRHLLGDGSQLGMRLCYQVQETPNGLAQAFVIGREFIGDD
                     PVCLVLGDNLFYGDGMRRMLAEADSGAGATVFGYYVTDPERYGVVSFDAAGRAETIEE
                     KPAQPKSNYAVVGLYFYDQDVVQIATELKPSARGEYEITDVNRVYLQQGRLNVKVMGR
                     GVAWLDTGTHDSLADATAFVKAIQDRQGLKIACIEEIAWRMGFIDRAQLERLAASLSK
                     STYGEYLAHLLKQEIRS"
     gene            complement(1307..2383)
                     /gene="rfbB"
                     /locus_tag="PK576_07730"
     CDS             complement(1307..2383)
                     /gene="rfbB"
                     /locus_tag="PK576_07730"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006662357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE61519.1"
                     /translation="MKRVIVTGGAGFIGSAVIRHLLDATDWQILNLDKLTYAGNLASL
                     PGAEDNPRYAFLRADICDRAAMEQAFRKFRPDAVMHLAAESHVDRSIDGPAAFVQTNI
                     VGTATLLEVALTYWRGLSDAEREAFRFQHISTDEVFGSLGPVGLFTETTPYDPRSPYS
                     ASKASSDHLVMAWHHTYGLPVMITNCSNNYGPFHFPEKLIPLVILNALEGKPLPIYGK
                     GDNVRDWLYVEDHARALRRVLERGVPGETYNVGGNTERTNLQVVETICATLDETCPER
                     LSGFGIQAFRELITFVPDRPGHDHRYAIDASKIRDRLGWAPQENFETGIRKTVAWYLE
                     NAWWWQPIRDKNYAGERLGRGTGS"
     gene            complement(2393..4837)
                     /locus_tag="PK576_07735"
     CDS             complement(2393..4837)
                     /locus_tag="PK576_07735"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOE61520.1"
                     /translation="MNEREDTTAGDVSTGTRRPIYYGGFNKRAIYYGGHAPSAYPGYP
                     GGGYGGGAYYYGMGGGVGDDTDSLMGTVTIGRMLRVCSQRWVTLVVFVIIGFIAAFAV
                     FKISPMIYEAESVFEMSIRGSTYTGMRKAIIETDVGSSLDEVFNTRLARLRSRAVIDQ
                     IVSQYRSDYPSSTVTDEQLIETLFESKMTLQRRSRLIRVTVRSTDPQLATDLANAYAK
                     AAETFTSDSNRNESEVAVSWLATTTEQQKRNLERADKEMLDFRVANQLDFMASESETT
                     QQARGKINADILVLEGEITKATELRKTLEQIQNEPEKFGTLPEAVPRSGEIGTAYQQL
                     QNVLAEKNSLLARYTANHPEVKVKEKEAEVFKQQFADVVFRALETCKANLDLLQRQLA
                     QLTPKRDELVKKLSDLELKMVETKMKLEQLEREREVADISYKSLLQRTREAQVASDEN
                     TAIIKQVEAAYRPTKPVLPNPMIIFPAGPLIGLLLGVVFVLVLDHLEDTIVGISDIEQ
                     RLRLKTLAVLPHVRRKKREQLARLVADDKFSQFAEAVAGLRNLLDSPRYQEMTKVLLC
                     MSTQPGEGKTIASCSLALSYAQSGQKTLLIDFDMRRPRLARIFEKRHSDFNSLPHTLA
                     KGDTSLFETLPVASGMENLDVVLSRASSELSPSSLMGSGVIVDFFSWAREHYDHVIID
                     SPPFGIVGDVMVLASLVDAVMILCCPDRTRFQPIKHAARHLTEAGARVIGVIVNDVDF
                     GRRNHFSHYDYHYRYAYRYTSRYGAYGQGKKPGDRTRAAAAGGAGTVRLDEDDLIPSG
                     LAARRDVVDVSMTDDE"
     gene            complement(4898..6304)
                     /locus_tag="PK576_07740"
     CDS             complement(4898..6304)
                     /locus_tag="PK576_07740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE61521.1"
                     /translation="MMQKILTKYGLAIHVALVCFFPFAGFGSSRWLPFIPFLWLSLAV
                     VELMILLPSVYRGETLADARQRMVRGLYWDPLLYIGAALVGMLMIQWLNGGCPLVYLP
                     DADVWRISTPPIAWAPFCVEAVAGFSQFCVLVACVVVGLAVRNALSRTPKRMLLQGMA
                     VASGCVALISVARVWPERCLADAANLARGTFFGFWMLLSWGLLADALTRFQRGGRTLY
                     LFGTLANLIGLLFYSPPLSVCLYGILGLALFLYALVYLDAQVSKKTQVLLFLVTVFAL
                     AALVVTLGFLMPDNPVAAKMKEAWPVAEYWEKLMATRRVRMDAALALWKEHLWFGCGA
                     GGFFHGVGLLLSDKEWALLQTDQAYAYHEYLQFLCEYGVVGLALFLTAALALIAPLCY
                     RARIAWQAGGEVGMEGRFFLLRISPIVVSGVLATGAVFVESWFANPFRSPAVLLSWSV
                     ALAALPGFLPKHANKKAR"
     gene            complement(6403..7605)
                     /locus_tag="PK576_07745"
     CDS             complement(6403..7605)
                     /locus_tag="PK576_07745"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOE61522.1"
                     /translation="MVTRTVFVARARIILMFALILVCGVTRAQERGFKFNNLTVSPYV
                     NLEYMYDSNVDSDRTEVSDEIFSVNPGVDLTYTGNDWGLSAGLWFGRDYYRDYDELDK
                     NRYGERVSFYRESAKGWRFVLGQSFIRSRENDSILDGGRGLWRDRDLHEINSALSYQI
                     SEKTGVTLSGMFSDLNYRNDQGRYAPLYGWREWSTGLEFARKLSEKSNVLLAGTYQQY
                     KSNGAVGGVDSSSSGYSLMAGFGSRATERISYRALTGVEWFDYAEGDLLTGWKYSLDA
                     SWLISKKWAATVAGSSYYQPSETQVNQAMQVHAISAGVTYRPMRKLTTRLDVAYRREE
                     DEYQNAVARNMGAATDDIFSIRARADYQLMRYVSVYGGLEFEDRSSDNRAYDYDQYRA
                     LLGLQFRY"
     gene            complement(7656..8654)
                     /locus_tag="PK576_07750"
     CDS             complement(7656..8654)
                     /locus_tag="PK576_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61523.1"
                     /translation="MKKSKRVLGCALGVGLAWAAVVGLAQQSEFLSRSQWLKKIGASV
                     TDAAVLRDTLEQVAPSDRVEFTQRLLKAVSRLPANPDEKGAAFVRSAVACIAGADAET
                     KRDVIAAVFAGVPVEYLPLVTDELAKRFNREYNQLTCEQYGMIATNVLGAVTVRNAET
                     DEASVRNTFTVLAFLKGADCPNLQRDLLAVLPDDRMRGLAQTWIPQVLNEGKYDAMLA
                     AADAEALPLRHEDILRLIGHASLDRLLADMLASLPADGKGASLSLEKARLGGASASSS
                     TAVSGSNLDYGINRVPRNPFYPSGYQNQKLTIIGDDFLQERGKRRVYRVKTFCGCP"
     gene            complement(8686..9336)
                     /locus_tag="PK576_07755"
     CDS             complement(8686..9336)
                     /locus_tag="PK576_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOE61524.1"
                     /translation="MLKCLGFGLLCWAVLSVNGCRTAAEDAMGFDEQAQYVEEGEPKI
                     KPGLTLRVAVTASGSEAVQEGLKEVNANGEILMPLVGAIRCEGLTIMELQEKIKTAYK
                     DYYIEPQVTVGFVYTENTGMKSPWGSVLLMGEIVRPGPVNMPSTRDLTVTRALMLAGG
                     ATALADKRHVRVTRREKDGSLKRFMVDIDKIGKEGRSDLDIALKPGDVVWVPESWY"
BASE COUNT         2026 a         3188 c         2418 g         1781 t
ORIGIN      
        1 tcaatcctga aaatcctgtc atcctgtcaa tttatcggac atgattttcc gctgcccggg
       61 cgcatatatc catgattaac atgatgttgt tcaatcctga aaatcctgtc atcctgtcaa
      121 tttataggca aggagttagg agtgtttcgc gcgcctttac tggcgcgaaa agaatccacc
      181 cggctgaggc ttgacgcttc tgtcatgctt ctgtatcgtg tcccgaacga ttgcgccacg
      241 ccagagtggt ggaatggcag acacaccgga cttaaaatcc ggagccgggt taaaccggcg
      301 tgagggttcg atcccctcct ctggcaccaa tattgcctcc cctgaacgtc ccggctcggg
      361 gcgcaatgct caggatcgga tttcctgctt cagcaggtgc gccagatatt cgccgtacgt
      421 ggacttgctc agcgacgccg ccaggcgctc cagttgagcg cggtcgatga accccatacg
      481 ccaggcgatc tcttcgatac aggcgatttt cagtccctga cggtcctgaa tcgccttgac
      541 gaacgcagtg gcgtccgcaa gcgagtcatg ggttccggtg tccagccagg cgacaccgcg
      601 tcccatgact ttgacattca gccggccctg ctgcagatag acacggttga catccgtgat
      661 ctcgtattcg ccgcgggccg aaggcttcag ctcggtcgcg atctgcacca catcctggtc
      721 atagaaatag agtcccacca ccgcgtaatt cgatttcggc tgcgcaggtt tctcttcaat
      781 ggtttccgcc cggccagccg cgtcaaaact gacgacacca taccgctcgg gatcggtcac
      841 gtaatacccg aacactgtgg cgcccgcgcc actatccgct tcggcgagca tccggcgcat
      901 cccgtcgccg taaaaaaggt tgtcgcccaa caccaggcag accggatcat caccgatgaa
      961 ctcgcgtccg atcacaaacg cctgcgccag cccgttcggc gtttcctgaa cctgatagca
     1021 caaccgcatg cccagttggg aaccgtcgcc gagaagatgc ctgaacacgg gcgtcgcttc
     1081 aggcgtggaa atgatcagca cctcccggat ccccgccagc atcagggtcg agaggggata
     1141 ataaatcatc ggcttgtcgt ataccggaag cagttgtttg caaatcacct gcgtgaccgg
     1201 aaacaaccgg gttcccgcac cgcccgccag aataatccct ttccgcgcca tcctcccacc
     1261 ctccttccca acatgaatcc gccgcttctc ctgaaccgaa cagcggtcag gacccagtcc
     1321 ctcggcccaa ccgttccccg gcatagttct tgtcgcggat cggctgccac caccaggcgt
     1381 tctccaaata ccaggccacc gttttccgta tgccggtctc gaagttctcc tgaggcgccc
     1441 aaccgagccg gtcgcggatt ttggaggcgt cgatcgcata ccgatgatca tggccgggac
     1501 ggtccgggac aaacgtgatg agttcgcgga aagcctgaat cccgaaaccg gacaaccgct
     1561 ccgggcacgt ttcgtcgagc gtcgcgcaaa tcgtctccac aacttgcagg ttggtgcgct
     1621 cggtgttgcc gcccacgtta tacgtctcgc ccggaacgcc ccgctccaga accctacgca
     1681 acgcgcgcgc atgatcttcc acatacagcc aatcccgcac gttatcgcct tttccgtaga
     1741 tcggcagggg ctttccttcc aacgcgttca ggatcaccag cggaatcagc ttttcaggga
     1801 agtggaacgg gccgtaatta ttcgaacagt tggtgatcat caccggcaac ccgtaggtgt
     1861 ggtgccaggc catgaccaaa tgatcggagc tcgctttact ggccgaatac ggcgaacggg
     1921 ggtcatacgg cgtggtttcc gtaaacaacc cgaccggccc caacgatccg aacacctcat
     1981 ccgtggagat gtgctggaaa cgaaacgcct cccgttccgc atcggacagg ccgcgccaat
     2041 acgtcagggc aacctccaac agggtggcgg ttcccacgat attggtttgc acaaatgcgg
     2101 ccgggccatc gatcgagcgg tcgacatgcg attccgcggc cagatgcatc acggcgtcgg
     2161 ggcggaactt tctgaacgcc tgttccatcg ccgcgcggtc gcagatgtcc gcccgcagaa
     2221 aggcataccg cggattgtcc tccgcccccg gcagggaagc cagattcccc gcataggtca
     2281 gcttgtccag attcaggatt tgccagtccg tcgcgtccag caggtgccgg atcacagccg
     2341 agccgataaa cccggcaccg ccggttacaa ttacccgttt catccgttct cctcactcat
     2401 catctgtcat ggaaacatca accacatccc ggcgcgcggc caagccggac gggatcagat
     2461 catcctcgtc cagccggacg gtccccgcgc cgcctgccgc ggccgcacgg gttctgtcgc
     2521 cgggtttctt gccctgtccg tatgcgccgt agcgcgatgt gtagcggtag gcataccggt
     2581 agtgataatc ataatggctg aaatggttgc gccggccaaa atccacgtca ttcacaatga
     2641 cgccgatcac ccgcgcgccg gcctccgtca gatgacgggc cgcatgtttg atcggctgga
     2701 accgtgtgcg gtcgggacag cacaggatca tcaccgcatc gaccagcgag gccaagacca
     2761 tgacatcccc cacgatcccg aacggcggcg agtcgatgat gacgtgatca taatgctcac
     2821 gcgcccaaga gaagaaatcg acgatcacgc cgctccccat caggctggag ggactcagct
     2881 ccgaagaggc gcgagagagc accacatcga gattttccat ccccgacgcc acggggaggg
     2941 tctcaaacaa cgaggtgtct cccttggcca gcgtatgcgg caggctgttg aaatccgaat
     3001 gccgtttctc gaaaatccgg gcgagccggg gacggcgcat atcaaaatcg atcagcaggg
     3061 tcttctgccc gctctgggca tacgacaacg ccagcgagca ggaggcgatg gttttgccct
     3121 cccccggctg cgtgctcatg cacagcagca ccttggtcat ctcctgatag cggggcgaat
     3181 ccagcagatt ccgcaatccg gcgaccgcct cggcgaattg cgaaaactta tcatccgcaa
     3241 ccagccgcgc caactgctcg cgttttttcc ggcgcacatg cggcagcacc gccaaggtct
     3301 tcaggcgcag acgctgctcg atatccgaaa tgccgacgat cgtgtcctcg aggtggtcca
     3361 gcaccagcac gaacacaacc cccaacaaca ggccgatcag cggtcccgcg gggaagatga
     3421 tcatcggatt gggcaacacc ggcttagtcg gacggtaggc ggcctccacc tgcttgatga
     3481 tcgccgtatt ttcatccgac gccacctgcg cctcgcgtgt acgctgcaac agcgatttgt
     3541 aactgatgtc cgcgacttcc cgctcgcgct ccaactgctc gagtttcatt ttggtctcca
     3601 ccatcttcaa ctcgagatct gaaagtttct tcaccaactc gtcccgtttc ggcgtcaact
     3661 gggccagttg gcgctgcagc agatccagat tcgccttaca ggtctcgagc gcacggaaga
     3721 ccacgtcggc gaactgctgc ttgaagacct cggcctcttt ctctttgact ttcacttccg
     3781 ggtggttggc ggtatagcgg gccaacagcg aatttttttc agccagcacg ttctgcaact
     3841 gctgataagc cgtgccgatc tcgcccgacc gcggcaccgc ttccggcagg gtaccgaact
     3901 tttccggctc gttctgaatc tgttccagag ttttccgcag ttctgtggcc ttggtgattt
     3961 ccccttccag caccagaatg tccgcgttga ttttgccgcg cgcctgctgc gtggtctcac
     4021 tctcgctggc catgaaatca agctggttgg ccacccggaa atccagcatc tccttatcgg
     4081 cccgctccaa gttccgcttc tgctgctccg tcgttgtcgc cagccacgag acggccacct
     4141 ccgactcgtt gcggttcgaa tccgacgtga aggtttccgc agccttggca taggcgttgg
     4201 cgagatccgt cgccaactgc ggatccgttg accgcaccgt gacccggatc agacgcgaac
     4261 ggcgctgcag cgtcattttc gactcgaaca acgtttcgat caattgctca tccgtgaccg
     4321 tcgaactggg gtaatcactc cggtactgac tgacgatctg atcaatcacc gcgcggctgc
     4381 gcaaccgggc caaccgcgta ttgaacacct catccagcga cgaaccgaca tcggtttcaa
     4441 tgatcgcctt gcgcatgccg gtatacgtcg acccgcggat gctcatttcg aacacacttt
     4501 ccgcctcata aatcatcgga gagatcttaa ataccgcaaa cgcggcgatg aagccgatga
     4561 tcacaaacac caccagcgta acccaccgct gcgagcacac gcgaagcatg cgcccgatcg
     4621 tgaccgtgcc catcagggaa tcggtgtcat ccccgacgcc tccgcccata ccataataat
     4681 atgcgccgcc cccgtatccc cctccgggat aaccgggata cgccgaaggc gcgtgtccgc
     4741 cgtaatagat ggcccgtttg ttgaacccac cgtaatagat cggtcggcgt gtacccgtgc
     4801 tcacatcccc ggcagtggtg tcttccctct cgttcatcta tcctctccca aaaaatgatt
     4861 tcgcggctgt tccgccaacc gatcacagat ggcacgctta gcgcgccttt ttgttcgcgt
     4921 gcttgggcag aaaaccaggc aacgcggcca aggccaccga ccacgacaac aacacagcgg
     4981 gcgaccgaaa ggggttggcg aaccaactct cgacaaagac ggcacccgtg gcgagcacac
     5041 ccgacaccac gatcggcgaa atgcgcaaca ggaagaatcg gccttccatt cccacctcgc
     5101 cgccagcctg ccaggcgata cgggcccgat aacaaagcgg cgcgatcaac gcaagcgccg
     5161 cagtcagaaa gagggccaac cccaccaccc catattcgca caggaactgg aggtactcat
     5221 gataggcata cgcctgatcg gtttgcagca gcgcccactc tttatccgag agcaacaatc
     5281 cgacaccgtg aaaaaaccct ccggccccgc aaccgaacca gagatgctcc ttccacaacg
     5341 caagcgcggc atccatacga acccgtcgtg tggccatgag cttctcccaa tactccgcaa
     5401 cgggccaggc ctccttcatc ttcgcggcca ccggattgtc cggcatcagg aaacccagcg
     5461 tcaccaccaa tgccgccaac gcgaacaccg tgaccaaaaa cagcagcacc tgcgtttttt
     5521 tggaaacctg cgcatccaga tacactaacg catacagaaa gagcgccaga cccaagatgc
     5581 cgtacaggca gaccgacaag gggggggaat aaaaaagcag gccgatcaga ttggcgaggg
     5641 tcccgaacaa gtacaaggtc cgccctcccc gctggaaacg cgtcagcgca tcagcgagaa
     5701 gcccccagga caggagcatc cagaacccga aaaacgtgcc tcgcgccaga ttcgccgcat
     5761 ccgccagaca cctctccggc cagacacggg ccaccgagat cagcgccaca cagccgcttg
     5821 ccacggccat gccctgcagc aacatgcgct tcggtgtccg gctgagcgcg ttccgcacgg
     5881 cgagccccac caccacacag gcgaccagga cacagaattg ggagaacccc gccacagcct
     5941 ccacgcaaaa cggcgcccag gcaatcggcg gagtgctgat ccgccacaca tcggcatccg
     6001 gaaggtacac gagcgggcat ccgccgttca gccactggat catcagcatg ccgaccagcg
     6061 cggcaccgat atacagcagc ggatcccagt acagaccgcg caccatgcgc tgacgggcat
     6121 ccgccagcgt ctcaccccga tacacagagg gcaacaggat catcagttcc acgaccgcca
     6181 gggaaagcca cagaaacggg atgaacggaa gccaacgcga actcccaaat ccggcaaaag
     6241 ggaaaaaaca aacgagggcc acatggatcg cgagcccgta ttttgtcagg atcttctgca
     6301 tcatacacgg aacatcacag gcaccgcata agaaatccgc ctacgcatcg catgcggtga
     6361 aggcggattc ggcgtgcgca tgattctcgt tcagcccatc gatcagtacc ggaactgcaa
     6421 tcccagcagc gcccggtact ggtcataatc atacgcacgg ttgtccgacg aacggtcttc
     6481 aaattccagc ccaccgtaca ccgacacata ccgcatcaac tggtaatcgg cgcgcgcgcg
     6541 gatcgagaag atatcatcgg tggcggcccc catattcctg gccaccgcgt tttggtactc
     6601 atcctcctca cgccggtagg ccacgtcgag acgcgtcgtg agcttacgca tcggccggta
     6661 ggtgacgccc gccgaaatcg cgtgcacctg catcgcctga tttacctgcg tctcgctggg
     6721 ctggtaataa ctcgaaccgg ccaccgtggc cgcccacttc ttgctgatca gccaactggc
     6781 gtccaaactg tacttccatc cagtcaagag gtcgccttcc gcataatcaa accactccac
     6841 accggtcaga gcccggtacg agatccgctc cgtggcccgc gaaccgaaac cagccatcag
     6901 ggagtatccc gaactgctgc tgtcaacgcc gcctacggcc ccgttactct tatactgctg
     6961 atacgtgccg gccagcagca cgttgctctt ttccgaaagc ttgcgggcga attccagccc
     7021 ggtgctccac tcgcgccagc cgtagagcgg agcgtatcta ccctggtcgt tccggtaatt
     7081 cagatcggaa aacatgccgc tgagcgtcac cccggtcttc tcgctgatct ggtaggacaa
     7141 cgcgctgttg atctcatgca ggtcccggtc ccgccacaac cccctgccgc catcgaggat
     7201 cgaatcattc tcccggctcc ggataaaact ctgccccagc acaaaacgcc atcctttggc
     7261 ggattcgcga tagaacgaca cccgttcccc gtaccggttc ttatccagtt catcatagtc
     7321 gcggtaataa tcacgaccga accagagccc ggccgacaac ccccagtcat taccggtata
     7381 cgtcagatcc accccggggt tcaccgagaa aatctcatcg ctgacctcgg ttcggtccga
     7441 gtccacattg gaatcgtaca tgtactccaa gttcacgtac ggcgagaccg ttaggttgtt
     7501 gaacttgaat ccgcgttcct gggctcgggt caccccgcac accagaatca gcgcgaacat
     7561 caggatgata cgcgcccgtg ctacaaaaac cgttcgtgtt accataaccc gtactccttg
     7621 cattaccgcg atctcaaagg gggacatcgt gttcgtcagg gacatccgca gaacgtttta
     7681 accctataca cccttctttt cccacgctcc tgtaagaaat cgtccccgat gatcgtcaac
     7741 ttctggtttt ggtatccgct cggataaaac gggttccgcg gcacacggtt gatcccgtaa
     7801 tccagattgc tgcccgaaac ggcggtcgat gaagatgcgc tggccccgcc caaacgagct
     7861 ttttccaacg aaagggacgc gcctttgccg tccgcgggaa ggctggcgag catatcggcc
     7921 aacaggcggt ccagcgaggc atgtccgatc agccgcagga tgtcctcgtg ccgcaacggc
     7981 aaagcctcgg catccgccgc ggccagcatc gcatcgtatt tgccctcgtt caacacctgc
     8041 gggatccacg tctgcgccag accgcgcatg cggtcatccg gcaacacggc aagcagatca
     8101 cgctgcagat tcgggcaatc ggcacctttc aagaacgcca ggaccgtaaa cgtgttgcgg
     8161 accgaggcct catccgtctc ggcattcctt acggtcacgg ctcccagcac gttggtcgcg
     8221 atcatgccgt actgctcgca ggtgagctgg ttgtattcgc ggttaaagcg cttggccaac
     8281 tcgtccgtca ccagcggcag atattccacg ggcacccccg cgaaaaccgc cgcgatcaca
     8341 tcccgcttgg tctccgcgtc cgccccggcg atacaagcca ccgccgaacg cacaaacgcc
     8401 gcccccttct catccgggtt ggcgggcaga cgcgagacgg ccttgagcag gcgctgcgtg
     8461 aactccaccc ggtccgaggg agcgacctgc tccagcgtgt cgcgcaacac cgccgcatcc
     8521 gtcaccgacg cgccgatctt cttgagccac tgcgaacgcg aaaggaattc gctctgctgc
     8581 gccaacccca caacagcggc ccaggccaag cccacgccca gcgcgcatcc taaaactctt
     8641 tttgactttt tcatctctct cagcctccct ccctctcttc gcggctcaat accacgactc
     8701 cggaacccaa accacatcac cgggtttgag ggcgatatcc aaatccgagc ggccctcttt
     8761 cccgatcttg tcaatgtcca ccataaaccg tttcaacgag ccgtccttct cccgccgcgt
     8821 cacccgcaca tgccgcttgt ccgccagagc cgtggccccg ccagccaaca tcagggcccg
     8881 cgtcaccgtc agatcccgcg tcgagggcat gttgacggga cccggccgga cgatctcacc
     8941 catcaacagc accgaacccc acggcgattt catgcccgtg ttctctgtat acacgaaacc
     9001 caccgtaacc tgaggctcga tgtaataatc cttgtaggct gttttgatct tctcctgaag
     9061 ctccataatc gtaaggcctt cgcagcggat cgcgcccacc agcggcatga ggatttcgcc
     9121 gttcgcgttc acttccttca gcccctcctg cacggcctcc gaaccggagg ccgtcaccgc
     9181 gacacgcaac gtcagcccgg gtttgatctt cggctctccc tcctccacat actgcgcctg
     9241 ctcatcaaac cccatcgcgt cctcggcagc cgtccggcac ccgttcacgg aaaggacagc
     9301 ccagcacagt aacccgaagc cgaggcactt caacatcacg ctcttcaagc caaactctct
     9361 catctataag ggcgtttcac acgacaccct tggttagcca ttgcccacag agt
//
LOCUS       DAOSLE010000105         9401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7006_length_9401_cov_7.514124, whole genome shotgun sequence.
ACCESSION   DAOSLE010000105 DAOSLE010000000
VERSION     DAOSLE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7006_length_9401_cov_7.514124"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..369)
                     /locus_tag="PK576_07760"
     CDS             complement(91..369)
                     /locus_tag="PK576_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61525.1"
                     /translation="MKKYKMNGTVTRQNNDTETLSNQAEIGSHRKYSRKQPTAEKILT
                     FRVEMATLFEWLSLHLRAGRYAAFVIGDSSACFVCALSGAQASPPAES"
     gene            368..2068
                     /locus_tag="PK576_07765"
     CDS             368..2068
                     /locus_tag="PK576_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE61526.1"
                     /translation="MKQILILCLAALVVALPFIFRPKPEEGGWLGDDPELVLITPHNE
                     ALRREFEVGFSHWHRLRYGRPVRFDWRVIGGTTEIMRYLTAEYEASLKRFQKSVSQRE
                     GLKRETGELAACGDDPAAVTCGIDLFFGGGVYDHAKAERQGLTVPAWPSGAEPAGLFK
                     DNDGRILIPREMNGEVWRGKAYYGSVLSAFGICYNADRLRDLGIRQPPAAWEDLADPR
                     YVGTLGLADPTKSGSVAKAFEMILHAQCVRSVAAAGFTREQIRQYEAAWATSGSAQDA
                     KPPEGVPAAYPEAIGCGWREGVNLVRRLGANARYFTDASGKVPSDVAMGAAAAGIVID
                     FYGRLQEELSARPDGRPVMTYVTPVGGSSMTADPISLLRGAPHRMLAVRFIEYLLGES
                     GQRLWNYRVGTPGGPKRFALRRLPIRRDFYPSSDPVLQAAFEQHRPFLADALWRPDAD
                     AYRLSEAFPYEARWTASHFGVQRDLIRAMCIDSGEELRRAWRAILRQGGPERQPEAMR
                     LLEALPEHPFPLSWATAVKGYSAYPRLDLLRQWTAFFREQYRQAEKVANSAADAYGGR
                     "
     gene            2098..3366
                     /locus_tag="PK576_07770"
     CDS             2098..3366
                     /locus_tag="PK576_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOE61527.1"
                     /translation="MPEYLHFGAGNIGRALAGPLFSRAGYRVVFVDAVPSIVAALQQR
                     KRYRVVVKDTIPAGESETFEVCGVDGISVEDAEAVRQAVARADLIGTAVGAAHLPDVL
                     TAIASGLPLRNGRPVSILFCENLNGLSVMAREVLTRALPPGFLMDRVGLVETSIGKMV
                     PLMPAEVRERDPLEVWGEAYNVIIADRNGFAGPVPEGVEGLELKSCFQAYVERKLYIH
                     NLGHATCACHGFLRGHTRICEAMADAAVVAETRQVMQVSARALIRRYPSEFSEANQTA
                     HVEDLLRRFGNRALGDTVFRVGRDLPRKLAPGDRFIGGLRLAQAEGEDPAPVCRAIAA
                     ALHFAAVDETGHLFPADAAFRERLAREGAEALLSAHSGLDPIRDREALNLILGELKPE
                     VNHPAQRANSSRATDNTFSTVNPKWRARSL"
     gene            complement(3288..5291)
                     /gene="tkt"
                     /locus_tag="PK576_07775"
     CDS             complement(3288..5291)
                     /gene="tkt"
                     /locus_tag="PK576_07775"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE61528.1"
                     /translation="MKVTNEALALAANTIRCLAMDGVQAARSGHPGAPMGLADIAAVL
                     WLKYLKHDPADSQWADRDRFVLSGGHGSMLLYSLLHLAGYALPLDEIKRFRQFGSLTP
                     GHPEYRHTDGVETTTGPLGQGIATAIGMAIAERMAAARFNVADAETVVDHRTWIFCGD
                     GDLEEGISHEACSLAGHLKLDRVVLFYDSNGITIEGKTELALSDDTKKRFQAYQWHVL
                     EIDGHDYDAIDRAIRKAMRLTGKPVMILCKTHIGKGSPNKQDSHESHGAPLGEEEVRL
                     TKRALGFPEDQFFYVPQEVYAMFQARAARMKRVHAKWTRTFKAWAKAHPERAELWTAM
                     REDRLPADIEERLPKFDPAKALATRSASGAVLNALAPLLPQLVGGSADLAPSNMTWLK
                     EMGEIKPGDFAGRNFHFGIRELAMAAILNGVQIHGGFRIFGGTFFVFSDYCRPAMRLA
                     ALMELPVIYVYTHDSFYVGEDGPTHQPVEQIAALRCMPNITVIRPADPTETAAAWVAA
                     LKNRKGPTALLLTRQNMRVIDRSRYPAASNLEKGAYTLWQSGDGTPDLILIATGSEVE
                     LALDAAQQITDRNVRVVSMPSWELFEKQNRTYRDRILDPDCKRRLVVEAGSAFGWERY
                     IGTQGARVTLDTFGASGAYKDLARHFGFTVENVLSVARELLAR"
     gene            complement(5453..6595)
                     /gene="hemW"
                     /locus_tag="PK576_07780"
     CDS             complement(5453..6595)
                     /gene="hemW"
                     /locus_tag="PK576_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE61529.1"
                     /translation="MTDPLYIHVPFCDGKCHYCGFYSVLNSTRLIEDYPRLPARELAH
                     LLDTRPAAVRTPIRTLYLGGGTPATLGESGLAELFRRLAEVTPLDSVEESTVELSPAS
                     VTPSLLNTLRRLGVNRLSIGVQCFNDAALARIGRRHDAATAIRAIRMAREAGFTNLGI
                     DLIAGLPEVTPEQWLDSLQQVLSLDLQHLSVYALTVEPGTALAYQAEKGLLLPDETAQ
                     LADLDQADTLLCRAGFTRYEISNYALPGFACQHNLAVWRGSDYIGLGPSASSRLGRDR
                     WTNERDLEGYTDALGRNASPPRMHETLTALDDATERTLFALRLKEGFDPAERAARFPV
                     LQEHILGWEKKLEALERHGITERAECRWRLTARGREVGDAVIRELL"
     gene            6977..8902
                     /gene="speA"
                     /locus_tag="PK576_07785"
     CDS             6977..8902
                     /gene="speA"
                     /locus_tag="PK576_07785"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HOE61530.1"
                     /translation="MPHKLLDALDGWNSVKSAELYGVSTWGQGYFNVTEDGYVAVNLK
                     NTDGTVSIRLCDIVDGLRDRGMGLPLLLRFSDLLGDRIRLLNEAFGKAIADYDYQGCY
                     RGVFPIKVNQQQQAVHDVVTFGAPYHHGLEAGSKAELIAALAYMRDPDAFLICNGYKD
                     TEFIDLALFSLKMGLQTILVLETADELSLILERAAELKIRPRIGIRVKLSSRVGGKWS
                     ESGGERSVFGLTPSQVIDMVDALREADMLDCLELLHYHLGSQVPNIREIRDSVKEASQ
                     FYVGLVKEGARMGIFDIGGGLAIDYDGSHTNFPSSANYDMSEYCADVIEGIMTACNEA
                     GVGHPTVISESGRALIGYYSVLLVNVLDAAIYEEKDAPANIPEPQPEALENMMYVSRT
                     LSLKNVQESFNDALYYREEIQKAFKHGDITLRQHAAADCLFWYILTRIRELVQNLKSV
                     PRDLQGLDTLLADVYYCNMSVFQSLPDAWAIDQLFPIMPIHRLNEKPTRMASLSDITC
                     DCDGNINKFIDQHDVRSVLPVHALRRGEPYMLAVFLVGAYQETLGDLHNLFGDTNAVS
                     VQVDEDGDIEYVQELAGDSVGDVLSYVEYRPERLMEQFRTLAEDAVKARRITPAERRE
                     ILAAYESGLRGYTYFEQ"
BASE COUNT         1791 a         2667 c         3079 g         1864 t
ORIGIN      
        1 cgttaaaccc ccaacttcca ttgtgccaga aacctttcac aaggagaaca gccttctcat
       61 cttacctctg tgttaaagaa ctttcaacgc tcaactttca gcaggcgggg acgcctgcgc
      121 tcccgacagc gcacacacaa aacacgcgct cgaatcgccg atcacaaaag cggcatagcg
      181 tcccgctcga agatgaagag acaaccactc aaatagcgtt gccatctcta ccctgaaggt
      241 aagaatcttt tcagccgtag gttgctttcg actgtacttg cgatggctgc cgatctccgc
      301 ttgattcgac aacgtctctg tgtcattatt ctgacgcgta acggtgccgt tcatcttgta
      361 cttcttcatg aagcagatcc tcattctttg tttggcggcg ttggtggtcg cgttgccgtt
      421 catcttccgg ccgaaaccgg aagagggcgg atggttgggg gacgatccgg aattggtgct
      481 gatcactccg cacaacgagg cgttgcggcg tgagttcgag gtgggcttct ctcactggca
      541 ccgtctccgc tacggtcgtc cggtgcgctt cgattggcgg gtgatcggcg gcacaacgga
      601 gattatgcgt tacctcaccg cagagtatga ggcttcgctg aagcgcttcc agaaaagcgt
      661 gtcgcaacgc gaggggctta aacgcgaaac aggcgaactc gcggcgtgcg gtgacgaccc
      721 tgcggcggtc acctgcggta tcgatctgtt ctttggcggg ggcgtgtacg atcacgcgaa
      781 agcggagcgg cagggtctga cggtgccggc ctggccaagc ggggccgagc ctgccggact
      841 ctttaaggat aacgacggca ggattctgat cccgcgggag atgaacggcg aggtgtggcg
      901 gggcaaagcg tattacggca gtgtgctgag cgcgtttggg atctgctaca acgccgaccg
      961 tctgcgggat cttggtatcc ggcagcctcc cgccgcttgg gaggatctgg ccgatccccg
     1021 ctatgtcgga accctcgggt tggcggatcc gacgaaaagc ggcagtgtgg ccaaggcgtt
     1081 tgaaatgatt cttcatgcgc agtgcgttcg atccgtggct gcggccggct tcacgcgcga
     1141 gcagatccgg cagtacgaag ccgcatgggc cacgtccgga agcgcacagg acgcgaagcc
     1201 gccggagggc gtgccggcag cctacccgga ggcgatcgga tgcgggtggc gggaaggggt
     1261 caatctcgtg cggcgtctgg gcgccaatgc ccggtatttc accgacgcgt ccggaaaggt
     1321 gccgtcggat gtcgccatgg gggcggccgc agcggggatc gtgatcgatt tctacggacg
     1381 cctgcaggag gagttgtccg cgcgtccgga tggccggccg gtcatgacgt acgtaacgcc
     1441 ggtgggggga tcgagcatga ctgccgatcc gatcagcctg ctgcgcggcg cgccgcaccg
     1501 catgttggcg gtccgtttca tcgaatacct gctcggcgaa tcggggcagc gtttgtggaa
     1561 ttaccgtgtg gggacaccgg gcggaccgaa acgttttgca ttgcggcggt tgccgatccg
     1621 gcgtgatttc tacccgtcct ccgatccggt gcttcaggcg gctttcgaac aacaccggcc
     1681 ctttctggcc gacgctctgt ggcgaccgga tgcggacgcg taccggttga gcgaagcctt
     1741 tccctatgag gcgcggtgga ccgcttcaca tttcggggtg cagcgtgatc tgatccgtgc
     1801 gatgtgtatt gattccggag aggagttgcg gcgcgcctgg cgggcgattc tgcggcaggg
     1861 cggtccggaa cgccaacctg aggcgatgcg gttgctggag gcgctgccgg agcatccctt
     1921 cccgctttcg tgggctacgg cggtcaaagg gtacagcgca tatccgcgat tggatctgct
     1981 gcggcagtgg accgcctttt tccgggaaca gtaccggcag gcggaaaagg ttgcgaattc
     2041 tgcggcggac gcgtatggcg gaaggtgaaa cgggaacagg gaaaggaagg acgggacatg
     2101 ccggagtatc tgcatttcgg ggccgggaac atcgggcgcg cgctggcggg tcctctcttc
     2161 agccgggccg ggtatcgggt ggtgtttgtg gatgcggtgc catcaatcgt ggcggcgttg
     2221 cagcagcgta aacgctaccg cgtggtggtg aaggacacga ttcccgctgg ggagtccgaa
     2281 acgttcgagg tgtgcggggt ggacgggatt tccgtggagg acgcggaggc ggtgcgtcag
     2341 gcggtggccc gtgcggatct gatcgggacg gcagtcggtg cggcgcatct tccggatgtg
     2401 ttgaccgcga tcgcatcggg actgccattg cgtaacggtc gtccagtttc gattctgttc
     2461 tgcgagaacc tgaacgggtt gtccgtcatg gcgcgtgagg tgttgacccg cgccctgccg
     2521 ccgggattct tgatggaccg tgtcggtctg gtggagacca gtatcggcaa gatggtgccg
     2581 ctgatgccgg ctgaggtccg ggaacgggat ccgctggagg tctggggcga ggcctacaat
     2641 gtcatcatcg ctgaccgcaa cgggtttgcg ggcccggtgc cggagggggt ggagggcctg
     2701 gaactgaaat cctgttttca ggcctatgtg gaacgcaaac tctacatcca caatctgggc
     2761 catgcgacgt gtgcctgcca cgggtttttg cgcggtcaca cacggatttg cgaagcgatg
     2821 gcggatgcgg ctgtggtcgc ggaaacgcgg caggtgatgc aggtctcggc gcgcgccctg
     2881 attcgccggt acccgtcgga gttcagcgaa gccaatcaga ccgcgcatgt ggaggatctg
     2941 ttgcggcgct tcggcaaccg ggcgttgggc gacaccgtct tccgcgtggg acgcgatctg
     3001 ccgcgcaaac tcgcgccggg cgaccggttt atcggcgggt tgcgcttggc gcaggcggaa
     3061 ggggaggacc ctgcccctgt ctgccgcgcg attgccgcag ccctgcattt cgcagccgtg
     3121 gatgagacgg ggcacctatt cccggctgac gccgcgttcc gcgaacggct ggcacgcgaa
     3181 ggtgcggaag ccttgctcag cgcgcacagc gggctcgacc cgatccgcga ccgtgaggcg
     3241 ctgaacctga tcctcggtga gctgaaacct gaagtcaatc accctgctca gcgggccaac
     3301 agttcgcgcg ccacggacaa cacgttctcg acggtgaacc cgaagtggcg ggccaggtct
     3361 ttgtaagcgc cggacgcgcc gaaggtgtcc agggtgacgc gggcaccttg ggtgccgata
     3421 taacgctccc atccgaatgc actgcctgct tccacgacca gacggcgttt gcagtcagga
     3481 tcgaggatcc ggtcgcggta ggtgcggttt tgtttttcga acagctccca cgagggcatg
     3541 ctgaccaccc gcacattccg gtccgtaatc tgctgcgccg catccagcgc gagttcgacc
     3601 tctgagccgg tcgcaatcag aataaggtct ggcgtaccgt cgccgctctg ccatagggtg
     3661 taggcacctt tttccagatt cgacgcggcg gggtaacgag agcggtcgat cacccgcatg
     3721 ttctgccggg tcagcagcag ggcggtgggg cctttacggt ttttcagcgc ggcaacccag
     3781 gcggccgcgg tttcggtcgg atccgcaggc cggatcacgg tgatgttcgg catgcatcgc
     3841 aaggcggcga tctgttcgac cggctggtgt gtgggaccat cctcgcccac gtagaagctg
     3901 tcgtgggtgt atacatagat caccggcagt tccatgagcg ccgccaaacg catggccggc
     3961 ctacagtaat cgctgaacac gaagaacgtg ccgccgaaaa tacggaaccc gccatggatc
     4021 tgtaccccgt tgagaatggc ggccatggcg agttcgcgga tgccgaagtg gaagttgcgg
     4081 cccgcgaagt cgccgggttt gatctcgccc atctccttga gccaggtcat gttgctgggc
     4141 gcgaggtcag ccgagccgcc gaccagttgc gggagcaacg gcgcgagcgc gttgagcacg
     4201 gcaccggaag cactgcgtgt ggcgagcgct ttggcgggat cgaatttcgg caaccgctcc
     4261 tcgatgtcag caggcagccg gtcttcgcgc atggcggtcc acaactccgc ccgttccgga
     4321 tgagccttgg cccaggcttt gaaggtgcgc gtccatttgg cgtgaacgcg tttcatccgc
     4381 gcggcgcgcg cctgaaacat cgcgtacacc tcctgcggca cgtaaaagaa ctggtcctcc
     4441 gggaatccga gcgcacgttt cgtgaggcgc acctcctcct cgccgagcgg cgcgccgtgt
     4501 gactcatggc tgtcctgctt gttgggactg cctttgccga tgtgggtttt gcagaggatc
     4561 atcaccggtt ttccggtcag gcgcatggcc ttacggatcg cccggtcgat cgcgtcgtaa
     4621 tcgtggccgt cgatttccaa aacatgccat tggtaggctt ggaacctctt tttggtgtca
     4681 tcgctgaggg cgagttccgt tttcccttcg atggtgatgc cgttgctgtc ataaaacagc
     4741 acaacccgat ccagcttcag atgcccggcc agcgaacagg cctcgtggct gataccttcc
     4801 tcaagatcgc catcgccgca gaagatccac gtgcggtgat cgaccaccgt ttcggcgtcc
     4861 gcgacattga agcgggcggc ggccatgcgt tctgcgatcg ccataccgat tgcggtggca
     4921 ataccctgtc cgagcggtcc ggtggtggtt tcgaccccgt ccgtatgtcg gtattccgga
     4981 tgtcccgggg taaggctgcc gaactggcga aagcgtttga tttcgtcgag gggcagcgcg
     5041 tatcccgcca gatgcagcag actgtagagc agcatggagc catgcccgcc ggagaggacg
     5101 aaccggtcgc ggtcagccca ttgggaatcc gccggatcat gcttcagata tttcagccac
     5161 agcacggcag cgatatcggc caggcccatg ggggcaccgg gatgtccgga ccgcgccgct
     5221 tggacgccat ccatggcaag gcagcggatc gtgttggcgg caagggccaa tgcttcattc
     5281 gtcactttca tgatgcgaca ctccttcttc agacttttca cggaaacgga aattacgttt
     5341 cactgtctca taaaaggggg caggatgtca aaagatcaag acgaaagaca agaaaaaggg
     5401 aaaaaaaggt agtgaggaag aaagggggaa gggaaacggg gatgagcccg cgctacagca
     5461 attcgcggat cacggcgtcg ccgacctccc gaccgcgtgc ggtcaagcgc cagcggcatt
     5521 ccgcccgttc ggtgatgccg tgccgttcga gcgcctccag tttcttctcc caaccaagga
     5581 tatgctcttg cagtacggga aaacgcgcgg cgcgttcggc gggatcgaac ccctccttca
     5641 gacgcagcgc aaacagggtt cgctcggttg catcgtccag cgcggtcagt gtctcgtgca
     5701 tccggggggg ggacgcgttt cgtcccaacg cgtccgtgta tccttccaga tcacgctcgt
     5761 tggtccatcg gtcgcggccg aggcgcgagg aggcggaggg acccagcccg atgtaatccg
     5821 aaccccgcca gaccgcgaga ttgtgctggc acgcaaaccc cggtagcgcg taattggaga
     5881 tctcatagcg cgtgaacccc gcgcggcaca ggagggtgtc cgcctgatcg agatcagcca
     5941 attgcgcggt ttcgtcggga aggagaagcc ctttctccgc ctgataggcc agcgcggtgc
     6001 cgggctctac ggttagggcg tagaccgaga ggtgttgcag gtcaagcgac aatacctgct
     6061 ggagagaatc cagccattgc tccggtgtga cctccggcaa accggcgatc agatcgatgc
     6121 cgaggttcgt gaacccggcc tcacgcgcca tgcggatcgc gcgaatcgca gtcgcggcgt
     6181 cgtgacgccg tccgatccgc gcaagcgcgg cgtcattgaa gcattggacg ccgatgctca
     6241 gccggttgac ccccaaccgc cgcaaggtgt tcaggagcga cggggtcacc gaggcggggc
     6301 tcagctcgac cgtgctctcc tccacggaat ccagcggcgt cacctcggca agcctccgga
     6361 agagttcggc gaggccagac tcgcccagtg tggcgggggt gccgcctcct aggtacaggg
     6421 ttcggatcgg ggtacgcact gcggcagggc gggtgtccaa gagatgcgcc aactcgcggg
     6481 cgggcaggcg ggggtagtct tcgatgagac gcgtactgtt caacacggag tagaagccgc
     6541 aatagtggca tttaccatca cagaacggaa catggatgta gaggggatcc gtcacgactc
     6601 aatcggtttc cttcacaggg gagaacgaga aggctcgtta tcctgtgcgg gcaaaacccg
     6661 caacacaagg attcgggcat cagaaaagag catacagaat gaaccgcgtc gaagcgagtc
     6721 gcttccgttg ttttcccgtt ttcgaccccg atgtttcagg tcaggagcag ttttctggga
     6781 gacaagggac gggcgtctgc ttctccttgc tcacgggaaa acaaatccgt gccatgtttt
     6841 ctccgtttta tgttgcactt tggtcgcggg tttggcatgt tgcgaccgtg tttaggaggt
     6901 gcgggaccgg cgcgttaaac ggggcgcgtc cgagtccgat ccgacaaagg atgtgatggg
     6961 ggaggaacca cccgaaatgc cgcataaact tttggatgca ctggacggct ggaacagcgt
     7021 caaaagcgcc gagctctacg gggtcagcac gtgggggcag ggctatttta acgtcacgga
     7081 agacggctat gtggcggtca acctgaaaaa cacggacggt acagtcagta tccggctttg
     7141 tgatatcgtt gatgggttgc gtgatcgcgg catgggactg ccgttgctgt tgcggttcag
     7201 cgatctgctg ggcgaccgta tccgtctgct caacgaagcg tttggcaagg cgattgccga
     7261 ttacgactat caggggtgtt accgcggcgt attcccgatc aaggtcaatc agcaacagca
     7321 ggcggtgcac gatgtggtga ccttcggggc accctaccat cacgggttgg aggcgggatc
     7381 caaagcggag ttgatcgcag cccttgctta catgcgggac ccggacgcct tcctcatctg
     7441 caacgggtac aaggacaccg agtttattga tctggcgttg ttctccctca aaatgggact
     7501 tcagaccatc cttgtgctgg agaccgccga tgagctgagt ctgattctcg agcgcgcggc
     7561 agaacttaaa atccggccgc gcatcggcat ccgggtgaag ctctccagcc gggtgggtgg
     7621 caaatggtcg gaatcggggg gcgagcgcag cgtgttcggt ctgacgcctt cccaggtgat
     7681 tgacatggtg gacgctctgc gcgaggccga catgctcgat tgcctggagc tgctgcacta
     7741 tcatctcggg tcgcaggtgc ctaacattcg cgagatccgc gacagtgtca aggaggcctc
     7801 ccagttttac gtcggtctcg tcaaagaggg cgcccgcatg gggatcttcg atatcggggg
     7861 cggtctggcg attgactatg acggctcgca caccaacttc cccagcagtg ccaactacga
     7921 catgtcggaa tactgtgccg atgtgatcga ggggatcatg accgcctgca acgaggcggg
     7981 agtcggccac ccgaccgtga tcagcgaatc cgggcgggcg ctgatcggct actactcggt
     8041 tctgctggtc aacgtgctgg acgcggcgat ttacgaggag aaggatgcgc ccgcgaatat
     8101 ccccgagccg cagccggagg cgctcgagaa catgatgtac gtctcgcgca cgctgagcct
     8161 caaaaacgtg caggagtcct tcaacgacgc cctatattac cgcgaggaga ttcaaaaagc
     8221 tttcaaacac ggcgacatca ccctgcggca acacgctgcc gccgactgcc tgttctggta
     8281 cattctcacg cgcatccgcg aactggtaca gaacttgaag agcgtaccca gggatctgca
     8341 ggggctggat acgctgctcg ctgatgtcta ctactgcaat atgagcgtgt tccagtcgct
     8401 gcccgatgcg tgggcgatcg atcagctctt cccgatcatg ccgatccacc ggttgaacga
     8461 gaagcccacg cgtatggcct ccctttccga tatcacctgc gattgcgacg ggaacatcaa
     8521 caaattcatc gatcagcacg atgtccggtc ggtcttgccg gtacacgcgt tgcggcgcgg
     8581 tgaaccgtac atgttggcgg ttttccttgt tggcgcgtat caggaaacct tgggagacct
     8641 gcacaatctg ttcggcgaca ccaacgcggt aagtgttcag gtggatgagg acggcgacat
     8701 cgaatatgtg caggagctgg caggcgactc ggtgggggat gtgctgagtt acgtcgaata
     8761 ccggcccgaa cgcctgatgg agcagttccg cacactggcc gaggatgcgg tcaaggcccg
     8821 gcgcatcacg cctgccgagc gtcgcgagat tctggccgcc tacgagagcg gcttacgcgg
     8881 ctacacctat tttgaacagt gatctgcggg gaaaagccgc tctgacgagc gtatccgaac
     8941 gccatgccgg tctgcgcatt taaaacctaa aaagagcggt tgaacgggta gaattccagc
     9001 aaagccccgt aaacattggg ttttgtcgcg gtccccgccg caccttgaga tgcgggcgga
     9061 gcccgcaatc tcaaaggctg tgagactgtg aggctgtgag actgtgaggc tgttaggctg
     9121 tgagactgtg aggcggtgag actgtgaggc tgtgaggcgg tgagactgtg aggctgttag
     9181 gcggtgaggc ggtggtgttc tgtggtgaaa caatcgatag cgatcccgat agcgatcccg
     9241 accccgattt ggatgaaacc ttgaaacctt gaaaccttga aacccgatcc cgatcccgat
     9301 agcgattccg accccgattt ggatgaaacc ctgaaacctt gaaacccgat cccgatcccg
     9361 atagcgatcc cgaccccgat ttggatgaaa ccttgaaacc a
//
LOCUS       DAOSLE010000106         9346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7043_length_9346_cov_6.031859, whole genome shotgun sequence.
ACCESSION   DAOSLE010000106 DAOSLE010000000
VERSION     DAOSLE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_9346_cov_6.031859"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    105..204
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            822..6098
                     /locus_tag="PK576_07790"
     CDS             822..6098
                     /locus_tag="PK576_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE61531.1"
                     /translation="MANVKGFTRKIWNSVPEFGRKIMAFVLMAGSLSAADGVWIAPGD
                     GAWGDTANWEGGAYASGAGFTAFFTNGVPAWIDQNITGLTLGGLYAAGADTLFTNSTV
                     TLNNNGNPATVTVDGPDVTATVALTLDPVAGTAITKAGSGTLRLDRYLKVPAGLTIEG
                     GILDLRFPGSSDGLNVGSGTITVKGGAILRCGAHNQINNSAVIDIQAGGTFDLAGMSD
                     NIGAVTGSGVLTNHSSTIQFWLDGTTRLFSGDCYGGGTFSFKQPGTFVVGGSNTLQNV
                     TFDGRFPGLNLSLAPGIGRFRIGNLHLTNDFALADTEGGPVALELGWKNDSFTLANVF
                     TGVGGLTKTGTGTMTVTNTQTYAGPTVVAEGKLTVTDETLANRLANTSQIQLGTGTVL
                     ELNTTESETNNTPVYGAGTIIKAKESTLALGALNTTNTAVTVSAGTLEVAGGTFSGGG
                     FTQNAGARLVFNGGSSTGATLTVAPGYPVEIRGGTHLFDSALGGTGRYLQTDGTTFFK
                     NTLGNNNTNDIFVTVSGGTMNIGYIQHCQGLGLLACGTAVVNIADNRTRIASDGKTHS
                     LVVTNDAQVTTDGLYLLSHGNTTSTGTVALAGGTLTMKGGFTGVGSQAGDLTYVSLDG
                     GLLRFIGSSSSLAGMYHLYQIREGGARIETATVDSTTTFYQGFTNATGGTIDGGLIKS
                     GPGTLRFLTDNRYTGASILNAGTLTSWGTGDTPFGCGPVTINGGTLEIAPSGSGANLA
                     LSLAHGDTANTVTVGPGVSMLRAARGNHVSLSVTLGAADAAADSVLTRDTHGVLAIWP
                     GAGTSTLGDTEKVIVNGGVATVNGVVPTVFGIHNDDRRSCDFLAYDAADGFKLAAYTA
                     GLGGGDTSIANVTANASTDSVHVHALRVHNGAVLTVNSGQTLTVGDGVNPAGVILNNS
                     SSVTAGLNGGTLDFGTSEGVIMYNLQRANQYGPTLGSVITGSNGLTLVGGGQAGGLQL
                     TAAANTYTGPTRIMAGRVSLANSRGFSDGDVYIYGNEAWGGQFRVTFTGNVTNTLHLA
                     GVGAGTSQQLGAVVFERDSEISGPIELMRDTRVSVSVLAGAIGTLSGPITGPGGLEIG
                     CPDAVIAGTVRLTGANTYGGGTRVSRGTLEIAAQDSLAPGQITVNNGVLAFALNGDVT
                     ITNAIRGVGRVVQKGSGTLTLTDAESFAGTFEIQAGALVVEGADTAMQTAFINGLLDL
                     DGQTVTTGILEGGGVVSNSTVTDAVLTVGTDDASSRFWGSILDGEGAVSLVKKGNGTL
                     TLSGLNAYGGSTTIEAGTLRLQGTQTELPTNGLAYWLDASRADLLTLDGSNVTMWADA
                     SGAGVHFTQEESVLQPLLVTNAASGLPAVRFGGRTNCLIAAKSVLSHSVFVVNEVFGY
                     RDNGGLWGGYGKDIGIRQSSLTSWIHPGNGNDFTCNGRMFVNGIEDRVFSSGVPHVLA
                     ALAEKPQNWVAAIGDYRPYSGSLFRSYRGDINEVAVYSTALGMSDMHSVSEYLRYKWL
                     GLESVFPTNVLPETTVLTLAAGGMLDLNGLNQAVGSLEGAGEVGNRSGRESTFTVGND
                     NRSTFFAGLISGSNALVKVGSGTLALGGANTYLGDTRVNGGTLKLDGGSLASGGTVTV
                     DAGATFDVNGQTQTLAGIGGSGTITGCDKLTVTETIAPGGVGEVGTLTLDGAPVFDSC
                     SLLIDTRKNGQADNLSVAGDLDLSALTLVIGDVTQMAGFSYEIVACSGSLTGTFAATP
                     NLPGAWVVRYDTTPGAGKVTLVHDIGTILLFR"
     gene            6173..7105
                     /locus_tag="PK576_07795"
     CDS             6173..7105
                     /locus_tag="PK576_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOE61532.1"
                     /translation="MVTIYHWEDGGLREVASPTGACWINLADPSTEEIARIQELTQVP
                     REFLTDPLDKDERPRLEIEDGATLLIVHVPYHEPHENLIPFHTLPLGIVLTQSHVITI
                     CNRLTPVTSTFLDQIRRVCPPEDQFRFTFQLLWHSAILYLRYLRDIRQRADMVEQDLH
                     ESISNEALMRLLNIEKSLVYFTTSLKANDILIGRIEHTRQLDIPDKDLDVLEDVAVEY
                     RQALEMATIHSNILTGTLDTFASIISNNLNNVMKYLTSITIVLMLPTLVASIYGMNVK
                     LPLDQYEYAFPILMGFSLLLSIVVVIVLARRRFF"
     gene            complement(7107..8258)
                     /locus_tag="PK576_07800"
     CDS             complement(7107..8258)
                     /locus_tag="PK576_07800"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOE61533.1"
                     /translation="MIVGMDVLAARRFDALHGRRVGLLSHQAALTRNGTTSAQCLRAA
                     LGNRLAALYGPEHGFFGQAGAGVHTTSRRHPDWGIPVHSLYGERRKPTPAMLSGLDLM
                     VCDLQDLGVRCYTYLATLRNMLEACRDAGLSVLVTDRPIPLPRAVDGPMPDADALGFV
                     TPCMVPLVYGMTPAESALWLRHTLFPDLRLDVVPMEGWTREDAAWDGQRPAFLPPSPG
                     IRTWESAMTYAATVFCEALSGIDCGRNTNLAFRVLGAPWLRAGQTCARLKSLRLAGVA
                     FTPYRYTAAGGTYDGRELDGILLTVTDPARFAPATVSLALLQTLAEQYGPRRVWHHKG
                     VRPTWFDTLYGTATTRRDLRGGRPFREIAAEWRTTQRAFQTARAAVLLY"
     gene            complement(8255..8899)
                     /locus_tag="PK576_07805"
     CDS             complement(8255..8899)
                     /locus_tag="PK576_07805"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOE61534.1"
                     /translation="MGRARKAEIGDRMVKRFPLYLRVLREMAEQDKPYASGAVVAKAL
                     SLDPIVVRKDFSRTGVRGTPRLGFPLREVIAAIERFLGWNAHTEAVLAGAGRLGSALL
                     GYPGFQNQGFKIVAAFDANPRKRGRIAGIPVFPADQLTNKSRALGVSLGILTVPADQA
                     QVAADAMMAGGIRGIWNFTPVQIAVPADVIVKHEDLAAGLAVLSHRLRQGGFIA"
     gene            complement(8953..>9346)
                     /locus_tag="PK576_07810"
     CDS             complement(8953..>9346)
                     /locus_tag="PK576_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61535.1"
                     /translation="ALIGPMIDLSVDDLRRQLALVLQDNFLFAATVMENIRYGRLEAT
                     DEEVMAAMEQEGVACFTKIPRVVGKGKATGPRLDNSVWPGANTLSLFVVPDERAQDVM
                     RVFEKLRATIGRKAGLKAFLLHVEQQTA"
BASE COUNT         1870 a         2756 c         2896 g         1724 t
ORIGIN      
        1 tcgatcccga tagcgatccc gaccccgatc ccgatttgga tgaaaccatg aaaccttgaa
       61 accccgaaac cctgaaacca gaaacccagg aaagtgtccc gtggnnnnnn nnnnnnnnnn
      121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnntgaaac agtcgatccc gatcccgata gcgaccccga
      241 tcccgatttg gatgaaaccc tgaaaccctg aaaccagaaa ccttgaaacc ccgaaacccc
      301 gaaacccagg aaagtgtccc gtggtgaaac agtcgatccc gatcccgata gcgaccccga
      361 tcccgatttg gatgaaacca tgaaaccatg aaaccctgaa accatgaaac cccgaaaccc
      421 agaaaccccg gaaagtgttc tgtggtgaaa cagtcgatcc cgatagcgat cccgaccccg
      481 atcccgattt ggatgaaacc atgaaacctt gaaaccccga aaccctgaaa ccagaaaccc
      541 aggaaagtgt cccgtggtga aacagtcgat cccgatcccg atagcgaccc cgatcccgat
      601 ttggatgaaa ccatgaaacc ttgaaaccct gaaaccatga aacccagaaa ccccgaaacc
      661 atgaaaccaa agagagttga aggttcaact gctcttttta agttgaaaga gcggactgaa
      721 gtgcaatgaa aagaacgaat gacagacttt ccgttttcag atattcttga ccgtgatttt
      781 ttgattgaac agggtttgtt cttcaggcaa ggaggcaggg tatggctaac gtaaaggggt
      841 tcacccgcaa aatctggaat tcagtcccgg aattcggacg gaagatcatg gcgtttgttc
      901 tgatggcggg ctcgctttcg gctgcggacg gcgtgtggat tgcgcccggg gacggagcgt
      961 ggggcgacac ggctaactgg gaaggcggtg cctatgcctc gggtgccggc tttacggcgt
     1021 ttttcaccaa cggggtgcct gcgtggatcg accagaacat tacggggctg acgcttggcg
     1081 ggctgtatgc cgctggcgcg gacaccttgt ttaccaacag caccgtgacc ctcaacaaca
     1141 acgggaatcc cgcaaccgtg acggtcgatg gacctgacgt caccgccacg gtggcgctga
     1201 ccctcgatcc cgttgccggt accgccataa ccaaagcggg gtcaggcacg ttgcggctgg
     1261 atcgatattt gaaagtgccg gcggggctga cgatcgaagg cgggatattg gacctccggt
     1321 tcccggggag cagcgacgga ctgaatgtgg gtagcggcac gatcaccgtg aaggggggtg
     1381 ccatactgcg gtgcggagcc cataaccaga tcaataattc cgcggtcatc gacattcaag
     1441 ctggcggtac gtttgatctg gcgggtatga gcgacaacat cggtgccgtg acgggcagcg
     1501 gtgtgctcac caaccactcc agcaccatcc agttctggct ggacgggacc acacgcctgt
     1561 tttccggaga ctgttacggt gggggaacgt tctcgtttaa gcagcccggc acctttgtgg
     1621 tcgggggttc caacacgctc cagaatgtga ctttcgacgg gcgtttcccg gggctgaatc
     1681 tttcgctcgc gccggggatc ggcaggttcc gcatcggcaa tctgcatctc accaacgatt
     1741 ttgcccttgc ggacaccgag ggtggtcctg ttgcgctgga gttgggatgg aaaaacgact
     1801 cgttcacgct ggccaacgtg tttaccgggg tgggcggcct gacgaaaacc ggaaccggca
     1861 cgatgacggt tacgaacacg cagacgtatg ccgggcccac ggtggtggcg gagggtaagc
     1921 tgacggtcac ggatgaaaca cttgcaaacc gcttggccaa cacctcgcaa attcagcttg
     1981 gaaccggaac ggttctcgaa ctcaacacaa cggaaagcga gacgaataac actccggttt
     2041 acggggcggg cacgatcatc aaggcaaagg aatcgacgct ggccctcggc gcgctgaaca
     2101 ccaccaacac ggcggtcacg gtttcggcgg gcacgctcga agtcgcgggc gggacgttct
     2161 ctggaggcgg gttcacgcag aacgcgggtg cccggctggt gttcaacggc ggcagcagta
     2221 cgggcgcgac cctgacggtc gcaccgggtt atccggtcga gatccgaggc gggactcacc
     2281 tctttgacag cgcgttgggc ggcacgggac gttatctcca gaccgacggc accaccttct
     2341 ttaaaaatac gcttggaaat aataacacca acgacatttt cgtcacggtt agcggcggca
     2401 ccatgaacat cggctacatc caacactgtc agggacttgg cctgttggca tgtggcacag
     2461 cggttgtgaa cattgctgat aaccgcacgc gtatcgccag tgacggcaag acgcactcgc
     2521 tggtggtgac gaacgacgcg caggtcacta ccgacggatt gtatctgctg tcacacggaa
     2581 acacgaccag tacaggaacc gttgctttgg cagggggcac ccttacgatg aaggggggct
     2641 ttacgggtgt gggatcgcag gcgggtgatt tgacttatgt gagtttggac ggtggtctgc
     2701 tgcggttcat cggttcctcg tcctctctgg cgggcatgta ccacctttac cagattcggg
     2761 agggcggtgc gcgtatcgag acagcgacgg tggatagcac gacgactttc tatcagggct
     2821 tcaccaatgc aaccggtggc acgattgacg gtggcctgat caagagcggc cccgggacgc
     2881 tccgattcct tacggataac aggtacaccg gagcctcgat ccttaacgcg ggtaccctga
     2941 cttcgtgggg aacgggcgac acgccgttcg ggtgcggtcc ggtgacgatc aacggcggca
     3001 cactggagat cgctccatcc ggaagcggtg cgaatctggc cctgtccctg gcccacggtg
     3061 atacggcgaa taccgtgacg gtcggtcccg gcgtgtcgat gctgcgtgcc gcgcgcggca
     3121 accatgtcag tctaagcgtg acgctcggcg ctgccgacgc ggcggcggac agcgtgctga
     3181 cgcgcgacac gcacggcgta ctggcgatct ggccgggagc cggcacgtcc acgctgggcg
     3241 acacggagaa ggtcatcgtt aacggcggtg tggcgaccgt caacggtgtg gtgccgacgg
     3301 tgttcggtat ccataatgac gaccgccggt cgtgcgattt cctcgcctat gacgctgcgg
     3361 acggtttcaa gctggcggcg tacacggccg ggctcggtgg cggcgacacc tcgatcgcca
     3421 atgtgacggc caacgcctcg acggacagtg tccatgtcca tgcgctgcgt gtgcacaacg
     3481 gcgcggtact gaccgtcaac agcggtcaga cgctgactgt cggcgacggc gtgaatcccg
     3541 ccggggtgat cctcaataac tccagcagcg tcaccgccgg gctgaacggc ggcacgcttg
     3601 atttcgggac atcggaaggc gtcatcatgt acaacttgca gcgggcgaat cagtacggcc
     3661 ccacgctcgg gagtgtgatt acgggttcga acggtctgac actggtgggc ggcggacagg
     3721 ccgggggctt gcagcttacc gctgccgcca acacctacac cgggccaacg cgcatcatgg
     3781 cgggccgcgt gtcgctcgcg aacagccgcg gcttcagcga cggggacgtg tacatctacg
     3841 gcaacgaggc gtggggcggt cagttccgcg tcacgttcac cggaaacgtc accaacaccc
     3901 tgcatctcgc gggtgtgggc gccggaacat cccagcagtt gggtgcggtc gtattcgaac
     3961 gcgacagtga aatctcgggg ccgatcgagc tgatgcggga cacgcgggtg agcgtgtcgg
     4021 ttctggctgg cgcgatcggg acgctcagcg gtccgatcac cggtcccggc ggcttggaga
     4081 tcggctgtcc tgatgcggtg attgcgggaa ccgtccggtt gaccggtgcc aacacctatg
     4141 ggggagggac ccgtgtcagc cgcggtacgc tggagatcgc cgcgcaagat tccttggcgc
     4201 ccggacagat aacggtcaac aatggggtgc tggcgtttgc ccttaacggc gacgttacga
     4261 tcaccaatgc gatccgcggt gtcggtcgtg tggtgcagaa gggatcaggc accctgaccc
     4321 tcaccgacgc ggaatccttt gcaggaacct tcgagattca ggcgggcgcg ctggttgtgg
     4381 agggtgcgga tacggcgatg cagacggcct ttatcaacgg gttgctcgac ctcgatggcc
     4441 agaccgtgac gaccggcata ttggagggtg ggggagtggt gagcaactcc acggtgaccg
     4501 acgccgtgct gacggttgga acggatgatg cgagcagccg gttctgggga tcgatcttgg
     4561 atggtgaagg tgctgtctca cttgtgaaga aggggaatgg tacgctgacg ttgagcggtt
     4621 tgaacgcgta tggcggttcc acgaccatcg aggcgggaac cctccgcttg cagggtacgc
     4681 agaccgaact gccgaccaac gggctcgcgt actggcttga cgcgtcacgc gctgacctgc
     4741 tgacgctcga tggatcaaat gtcaccatgt gggcggacgc ttccggggct ggcgtccatt
     4801 tcacgcagga agagtcggtg ctacagccgc tgttggtgac gaacgcggcg agtggtttgc
     4861 ccgcagtccg ctttgggggc cggaccaact gcttgatcgc tgccaagagt gtgttgtcac
     4921 attcggtttt cgtggtcaac gaggtgttcg gataccggga caacgggggg ctttggggtg
     4981 gctatggaaa ggatatcggg atccggcagt ccagcctgac ttcgtggatc catccgggca
     5041 acggcaatga ctttacgtgc aacggacgca tgttcgtcaa cggtatagaa gacagggtgt
     5101 tctcatccgg cgtgccgcat gtgctggccg ctttagccga aaagccgcag aactgggtcg
     5161 ccgcgatcgg agattaccgg ccctacagcg gatcgctttt ccgttcgtat cggggcgata
     5221 tcaacgaagt ggcggtctac agcacggcgc tcggcatgtc ggacatgcat tcggtctccg
     5281 agtacttgcg atacaagtgg ctcgggcttg aaagcgtctt ccccaccaat gtgttgcccg
     5341 aaaccacggt cttgaccctt gccgcaggcg gcatgctgga cttgaacgga ttgaatcagg
     5401 cggtcggctc gctggagggc gcgggtgagg tgggcaaccg tagtggccgc gagtcaacgt
     5461 ttaccgttgg taacgacaac cgcagcacgt tcttcgccgg tttgatttcg ggctccaacg
     5521 cgctggtcaa agtcggcagc ggcacattgg cgctgggtgg cgcgaacact tatctgggcg
     5581 atacgcgcgt gaacggcggg accctgaaac tggacggcgg gagcctcgct tccggcggga
     5641 cggtcacggt cgacgcgggc gcgacctttg atgtgaacgg tcagacgcag acgctggccg
     5701 ggatcggcgg cagcggaacc atcaccggct gtgacaagct gaccgtgacg gaaacgatcg
     5761 cgccgggcgg tgtgggcgag gtgggcacgc tgacgctcga cggggcaccc gtattcgaca
     5821 gttgctccct gctcatcgac acgcgcaaga acggtcaggc ggacaacctt tccgttgcgg
     5881 gggatttgga tctctcggcg ctcacgctgg tgatcggtga tgtcacgcaa atggcgggtt
     5941 tctcgtatga gatcgtcgcc tgttcgggaa gcctgaccgg caccttcgcc gccacgccta
     6001 acctgcccgg cgcgtgggtg gttcgctacg acaccacacc gggtgcgggc aaggtcacgc
     6061 ttgtgcacga tatagggaca attctgctgt tccggtaacc atcaagaaac cgcttgcatc
     6121 aggtgggcga taagagtaca aacatgcccg ctgatgcgga gaggtggtgg ttatggtcac
     6181 aatttatcat tgggaggatg gcggcctccg cgaggttgcc tcaccgaccg gagcctgctg
     6241 gatcaatctg gccgatcctt ccacagaaga gatcgcgcgg attcaggagc tgacccaggt
     6301 tccacgcgag tttctcacgg atccgctgga caaggacgag cgaccgcgtc tggaaatcga
     6361 ggacggggcc acgctcctga ttgtgcatgt cccgtatcat gagccgcacg agaacctgat
     6421 cccgtttcat acgctgccgc tcggtattgt cctcacccag tcccatgtga tcacgatctg
     6481 caaccggctg acgcccgtta ccagtacctt tctggaccag atccggcgtg tctgcccgcc
     6541 cgaggaccag ttccgtttta cgttccagct tctctggcat tcggccatcc tctatctgcg
     6601 gtatctgcgc gacattcggc agcgcgccga catggtcgag caggacctgc acgaatccat
     6661 ctccaacgag gcgctgatgc gtctgctgaa cattgagaag agtctggttt actttaccac
     6721 ctccctgaaa gcgaacgaca tcttgatcgg ccggatcgaa catacccgcc agttggacat
     6781 ccccgacaaa gatctcgatg tgctggagga tgtggcggtc gaataccgtc aggcactgga
     6841 gatggccaca atccacagca acatcctcac cggcacgctc gacacgttcg cttcgattat
     6901 ttcaaacaat ctcaacaacg tgatgaagta tctgacctcc atcacgatcg tgcttatgct
     6961 gcccacgctg gtggccagca tctacggcat gaatgtgaag ctgcctctgg accagtatga
     7021 atacgctttc cccatcctca tgggcttttc gctgctgctc tcgatcgtcg tggtgattgt
     7081 gctcgcccgc cgccgtttct tctgagtcaa tacagcaaca ctgcggcgcg cgcggtctgg
     7141 aacgcgcgtt gcgtggtgcg ccattccgca gcgatttcgc ggaacggccg ccccccgcgc
     7201 aggtcccgac gcgtcgttgc cgttccgtac agcgtgtcga accaagtcgg gcgcacgcct
     7261 ttgtggtgcc agacgcgacg cggaccgtac tgttccgcca gagtctgcag caatgctagc
     7321 gaaacagtgg ccggagcgaa acgtgcggga tcggtgaccg tcagcaggat gccgtcgagt
     7381 tctcggccgt cgtatgtgcc cccggccgcc gtgtatcggt agggggtaaa ggcgacgcct
     7441 gccagacgca gggacttcag ccgcgcgcag gtctgtcccg cgcgcaacca gggcgcaccc
     7501 agaacgcgga acgccagatt ggtgttgcgg ccgcaatcga tcccggatag cgcctcgcag
     7561 aagaccgtgg ccgcataggt catggcgctt tcccaagtcc ggataccggg ggagggcggc
     7621 aggaaggcgg gacgctgacc gtcccaggcc gcatcctcac gggtccagcc ctccatcgga
     7681 accacatcga ggcgcagatc gggaaacagg gtgtggcgca gccagagagc actctccgcc
     7741 ggcgtcatgc cgtaaaccag cggaaccatg cagggcgtga caaacccgag cgcgtcggca
     7801 tccggcatgg ggccatcgac cgctcggggc aacgggatag gacggtcggt gaccagcaca
     7861 gacagacccg cgtctcgaca cgcctccagc atgttgcgga gggtggcgag ataggtgtaa
     7921 caccgcacgc cgagatcttg cagatcgcag accatgagat ccaaccccga aagcatcgcg
     7981 ggcgttggtt ttcgccgttc cccatagagg gaatgaaccg ggatccccca gtcgggatgc
     8041 cgacgcgaag tcgtgtgtac gcccgcgccc gcctgaccga agaaaccgtg ctccggaccg
     8101 tagagggccg ccagccggtt gcccagtgcg gcgcgtagac actgcgccga tgtcgtgccg
     8161 ttacgcgtca gtgcggcctg atggctcaac agccccacgc gtctaccgtg cagggcgtcg
     8221 aagcgtcgcg cggccagcac atccatgccc acaatcatgc gatgaaacct ccttgccgca
     8281 atcggtggga gagcaccgcc agtccagcgg ccagatcttc gtgtttgacg atcacatccg
     8341 caggcaccgc gatctgcacg ggcgtgaaat tccagatgcc ccgaatcccg ccggccatca
     8401 tggcatccgc cgcaacctgc gcttggtcgg ccggcaccgt gagaatcccg agcgacacgc
     8461 ccagtgcgcg ggatttgttg gtgagctgat cggcgggaaa gacgggtatc cccgcaatcc
     8521 ggccgcgttt ccgcggattc gcgtcaaagg cggccacgat cttgaatcct tggttctgaa
     8581 acccggggta tcccagcaaa gcggagccta gacgtcccgc gccggccagc accgcttcgg
     8641 tatgggcatt ccagccgaga aaacgttcga tggcggcgat cacctcacga agcggaaagc
     8701 ccaagcgcgg cgtgccgcgc acaccggtcc gggagaaatc tttgcgcacc acgatcggat
     8761 ccagcgacag cgctttggca accaccgctc cggaagcata gggtttgtcc tgctccgcca
     8821 tctcccgcaa aacccggaga tacaagggaa atcgtttcac catccggtcg ccgatctcgg
     8881 ccttgcgcgc tctgcccatg ttgtgcccga ccctttccgc ggttcgccgc aaacggccac
     8941 gtacccacac ctttaagcgg tttgctgctc cacatggaga agaaatgcct taaggcccgc
     9001 tttacgcccg atggtggcac ggagtttttc gaaaacccgc atcacatcct gcgcgcgctc
     9061 atccggcacc acgaacagcg acagcgtgtt cgcacccggc catacgctgt tatccagccg
     9121 cggcccggta gccttgcctt ttcccacaac ccgcgggatc ttggtgaagc aggcgacccc
     9181 ctcctgttcc atcgccgcca tgacctcctc atcggtcgcc tccaggcggc cgtagcggat
     9241 attctccatc actgtagcgg caaagagaaa gttgtcctgc aggaccaggg ccaactggcg
     9301 ccgcagatca tccacggaca gatcgatcat cggcccgatg agcgca
//
LOCUS       DAOSLE010000107         9332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7053_length_9332_cov_6.746470, whole genome shotgun sequence.
ACCESSION   DAOSLE010000107 DAOSLE010000000
VERSION     DAOSLE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7053_length_9332_cov_6.746470"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..1198)
                     /locus_tag="PK576_07815"
     CDS             complement(398..1198)
                     /locus_tag="PK576_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOE61536.1"
                     /translation="MTYTPSASPSNVRGIICDMDGVIYHGNALLPGTRPFIDWLRQSK
                     TPFLFLTNSSERSPRELSEKLSRLGVLIEESHFYTSAMATAAFLASQTPGGSAYVIGQ
                     PGLIHALYEAGFSINDVNPDYVVVGETPMYTYETIKKSVSLVRNGAKLIGTNPDLTGP
                     VEHGIIPACGALVAPIELATGMKAYFVGKPNPLMMRSALRLLGCPRESALIVGDRMDT
                     DILSGIEAGMQTVLVLSGVTRREDLVRYSYRPNHVLDHVGLLPDLLGG"
     gene            1491..2273
                     /locus_tag="PK576_07820"
     CDS             1491..2273
                     /locus_tag="PK576_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE61537.1"
                     /translation="METNKRKLLLVDDDPSLLETLGDFLMFEGYEVLCAASGEDALVK
                     MRAFQPDLIILDMGMPGMGGTGFLDRITQPDGVPLYPVLVLTARASMAEYFADKQISG
                     FIAKPCDPSDLLMEVSRILFMSPADGRHAASQRVVRRLVIGEGDAALRETLRREFTLA
                     GFEVEVCATGPETLEACITLKPHAVVMRLELDGMSADEVVGMLHRLPGARQIRAVVYG
                     MDLPEAQLEHVANLDIPQSGMIRDLTVNTILDRTMAVTGGAA"
     gene            2299..2745
                     /gene="nrdR"
                     /locus_tag="PK576_07825"
     CDS             2299..2745
                     /gene="nrdR"
                     /locus_tag="PK576_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOE61538.1"
                     /translation="MRCPKCGHLDDKVLDSRAAREGASIRRRRQCLACEHRFTTYEEI
                     VKDELRVIKRDGRREEFSRLKLERGVMRACEKRPISADQIHDLVDEIIEGFEGGSEVP
                     SERIGQAVMTRLHKLDEVAYIRFASVYRRFADVSQFLKAVQDMVES"
     gene            2761..3078
                     /locus_tag="PK576_07830"
     CDS             2761..3078
                     /locus_tag="PK576_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HOE61539.1"
                     /translation="MLKLTVYIPETHLESVKAALFAAGAGRYAAYDHCCWQVLGEGQF
                     RPLAGSSPYIGNTGSVTRVAEYRVEMICAEAECAAVTAALRAAHPYEEPAFDFVRLCE
                     GSG"
     gene            complement(3168..4475)
                     /locus_tag="PK576_07835"
     CDS             complement(3168..4475)
                     /locus_tag="PK576_07835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOE61540.1"
                     /translation="MVSVIDLWQYEGIDFLQRYVGAKFGLLGIEPYHATADWWLNGID
                     KAFHETHFKGVPRPPGVGCNSPCLLLFYMPFTWFDFTTARYVSGALEWLAFLSVLCLM
                     TRLIPRNRDRALTFSLILFFTAGAPFWRLHVERGQYYVFTCLLIASSLVAHIKSNRPF
                     LSGLLLGLTVVIRPTVAFLALPFALQRRWRMLTGIACACLFMGGASLAVFGVEGWRQF
                     IWISEKYEIASTDFETDHFADLLRGRDPLPIQDFEQSRKQGLAMLPSINSTAAGMVRL
                     SKPLLDRCSIPVTSVLFLAKLVGVSTAVALILAALYTRSRTPWPFAYLAYVGIVAVNL
                     VEYAIPYRAAYADIYYLLPVMLGIPIVLRHRSVWLGVLLLVALASQQNLLGLVHGWYA
                     IPVRVAGLTLFSLGVLWHWRQSIISAPSAFDVTGETGSSSVPA"
     gene            complement(4634..5407)
                     /locus_tag="PK576_07840"
     CDS             complement(4634..5407)
                     /locus_tag="PK576_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE61541.1"
                     /translation="MTKKKIVVVMPAYNAAKTLKKTYDEVMCQGIVDEIILVDDASTD
                     ETVTLAATLPKVRVHVHPKNAGYGANQKTCYRLALESGADIVVMVHPDYQYTPKLIPA
                     MVSLIGSGLYPCVLGSRILGGYALKGGMPFWKYFSNRALTLFMNLLFGSKLSEFHTGY
                     RAFSRSLLERLPLQANMDDFAFDAQMLAQILWSGALIGEISCPTRYFEEASSINFQRS
                     VKYGFGCLATALEYRFCKEKLLSSHRFPRELSENAGRKP"
     gene            complement(5462..5635)
                     /locus_tag="PK576_07845"
     CDS             complement(5462..5635)
                     /locus_tag="PK576_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61542.1"
                     /translation="MVRKPGGIGSKEPSCELGLLAAQNLCKGTCHDLWAINTDYEYQE
                     MSRITETGLIQIV"
     gene            5724..8294
                     /locus_tag="PK576_07850"
     CDS             5724..8294
                     /locus_tag="PK576_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61543.1"
                     /translation="MKKRFRFSGGSRWLEGLGLLFVMLLFWGSMLYVFYPVLSRDWVM
                     LSPDFPKFYPTCAQAKWLEMVLAGRLSVVPMHALKFLGHPLIWQEFFFMLSTGLAGVA
                     VAYYLRTQRVPLVPACGGGLCFALSGYSFTLFCAGHGGYFYLISCGLFAFGLVNRCFQ
                     TRAFFYFALLGAVLMWAEIEQPDIWLLFVMLLAAYTLWRSYREWRESGSGSFIWRVVP
                     RFFLTLAVMLLIGFQQIRSAFTESLMNRTLQMEEHSSVTEQEQKTDPVQAEARKREER
                     WIFATNWSLPPEDMLEFIIPSLFGNDSFHMPHPYWGRLGRPHASVFQKGRMMPNYRQH
                     VVYLGVVAVLLAIFALFAWCAARRQEETGNQRTDDAGGPTGQGRQATRVSVFADVPFW
                     VGVWGVCAMISLGRYTPFYRLVYALPFMEYLRAPVKFHHLVELANACLAGWGLYALSS
                     SDVSTRRLRQRFAVFAAGVAGCLVVLALVLQVGAAGMERHITELGMGQVASALRGFAV
                     ANTVRSALLTLAVAGVFAGSAWRGVSLRRSLFCVCLLTALNTADLAVVARRCVTPVNV
                     GPHHAANLVVKTMLSRTGGRPANVLNYVTSNVWDQDWFHTALELHGFACVGPDPQNRS
                     APRSLLFEAFQRSPLRYWQVMGARFVLLPRKHAEPFFRQNILTAVCDFEIGRDTVRTS
                     QPTETSLVLAEVKGLPALPALYFEWEGPVAGDQQLQAVRAKESVGHLVADVDGVRGVA
                     TGSAVAPRSVVLDTLRKMPRALETRGLVETDRPALLVLGETYDAGLEALVDRVPVRVC
                     QANGMWAAVPVPAGKHEIVVRMKRNWGLNALSVATSLTLLGWFAVRLARRNVGRRRAT
                     "
     gene            8320..8949
                     /locus_tag="PK576_07855"
     CDS             8320..8949
                     /locus_tag="PK576_07855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE61544.1"
                     /translation="MGEGDRYYSNARPEMARFIHGRPSRILEIGCAGGCFRSNIFWPC
                     EYWGGEPVPDVAARASQTLSRVLTGTYEAVAAELPEGYFDLIVCNDVIEHMLDPDAFL
                     RSVRTRLNPQGQLVGSVPNVRYLLNLINLLIMKEWRYEAGGVLDATHLRFFTMKSLAR
                     LLRETGFRIERLQPVGPDRYLRLKRCLEIVAWWMGTDVLYAQMGFRCRI"
     gene            9003..>9332
                     /locus_tag="PK576_07860"
     CDS             9003..>9332
                     /locus_tag="PK576_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOE61545.1"
                     /translation="MSYLVVGSGFYGATMAERIASVLGERVMVIEKRAVVGGNSFSRA
                     DEETGIEIHLHGSHIFHTRDSRIWEYVRSFGAFNAYRHHVLASHHGRVYPMPVCLWTL
                     NQFFGRVF"
BASE COUNT         1919 a         2363 c         2993 g         2057 t
ORIGIN      
        1 tattgagatg cgggcggagc ccgcaatctc aaaggctgta agactgtgag gctgttaggc
       61 tgtgagactg aatcgatccc gatcccgata gcgatcccga tttggatgaa accttgaaac
      121 acatgaaact tcatgaaaat caggaattcc ccctggcgtt cccaaaaaac ccgagcgatg
      181 ccccaaaaat ctgcgtaatc tgtgtaaatc tgtggataaa aacccgagca gtgccatccc
      241 gtgtgttcgt ggttaaatga tcgatcccga tcccgatccc gatagcgatc ccgatttgga
      301 tgaaaccttg aaacccagaa accctgaaac catgaaaccc tgaaaccaaa gagagttgaa
      361 ggttcaactg ctctttttag gttaaaccgc ctggcggtca gccgccgagc agatccggca
      421 gtagaccgac gtgatccagc acatggttgg ggcggtagct gtaccggacg agatcctcgc
      481 gtcgtgtcac gccggagagc accagaaccg tttgcatgcc cgcctcgatt ccggaaagga
      541 tgtccgtgtc catacggtcc ccgacgatca gggcggattc gcgcgggcac cccagcaggc
      601 ggagcgccga gcgcatcatc agcggattgg gtttgcctac gaaataggct ttcatgccgg
      661 tggccagttc aatcggggcc accagcgcgc cgcaggccgg gatgatgccg tgttcgaccg
      721 gtccggtcaa atcggggttg gtgccgatta gtttggcgcc gttacgcacg agtgagacgg
      781 atttcttgat ggtctcgtag gtgtacatgg gggtttcgcc caccaccacg tagtccgggt
      841 tgacgtcgtt gatggagaac cccgcctcgt agagcgcgtg gattaggccg ggttggccga
      901 tcacatacgc gctgccgccc ggcgtctggg atgcgaggaa ggcggcggtc gccatggcgc
      961 tcgtatagaa atgcgactct tcgatcaaga cgcccaatcg cgaaagtttc tcgctgagtt
     1021 cccgcggcga acgttcgctg ctgttggtca ggaacaggaa gggagttttg gattggcgca
     1081 gccagtcgat aaacggccgc gtgccgggta acagcgcgtt tccgtgatag atcacgccgt
     1141 ccatatcaca gatgatgccg cggacattgg aaggggatgc ggagggggtg taggtcatag
     1201 ggactcctgg agggcgttga aatgcgtaca tgtgccatac acggggaatt ggacccaaag
     1261 cgcgtctaac tcctcttgca tggcggccac caaagcgatt ggtttttgca tgccggtcag
     1321 tttcgtatga tttggagaga gtgtcaaccg tttgcggtgg cctgtcaaca attcgacgtt
     1381 tgcataaagc atcttatggc gcgattacaa agaaaactcg atatgggtga attcggttgt
     1441 aaccttatcc ccttgaaacc gttgcggata cgggatacac tctccggcat atggaaacga
     1501 acaaacgcaa actgttgttg gtggatgatg acccgagcct gctcgaaacg ttgggcgact
     1561 ttctgatgtt tgaggggtac gaggtgctgt gtgcggccag cggggaggat gcgctggtca
     1621 aaatgcgggc gttccagccg gatctcatca ttcttgacat gggcatgcca gggatgggcg
     1681 gcacgggatt cctcgaccgg atcacccagc cggacggggt gccgctctat ccggttctgg
     1741 tgctgacggc gcgcgcctcg atggcggagt atttcgccga caaacagata tcgggattca
     1801 ttgcgaaacc gtgcgatccc tccgatctgc tgatggaggt cagccggatt ctcttcatga
     1861 gtccggcgga cggccggcat gcggcatccc agcgggtggt gcggcggctg gtgatcggcg
     1921 aaggggacgc cgccttgcgc gagacactgc gccgggagtt tacgctcgcc ggattcgagg
     1981 tggaggtttg cgctaccggg ccggaaacgc tcgaggcgtg tatcacgctc aagccccatg
     2041 cggttgtgat gcggctggaa ctcgatggga tgagcgcgga cgaagtggtc ggcatgctgc
     2101 accggttgcc gggcgcgcgg cagatccggg cggtggtgta cgggatggat cttcccgagg
     2161 cgcaactgga gcatgtggcg aatctggata ttccgcagag cggaatgatc cgggatttga
     2221 ctgtgaatac gattcttgat cggactatgg ctgttactgg cggcgccgcc tgacaaagaa
     2281 ggcggaaagg gaggtgcgat gcgttgtccc aaatgcgggc atttggacga taaggtgttg
     2341 gacagccggg cggcgcgcga aggcgcgtcg atccggcggc gacgccagtg tctggcgtgc
     2401 gaacatcggt tcaccacgta tgaggagatc gttaaagacg agttacgggt gattaagcgg
     2461 gacggacgcc gggaggagtt cagccgtctg aagttggagc gcggagtgat gcgggcgtgt
     2521 gagaagcgtc ccatcagcgc ggatcagatt cacgatctgg tggacgagat catcgaaggg
     2581 ttcgagggtg ggagcgaagt tccctcggaa cgaatcgggc aggcggtgat gacgcgtttg
     2641 cacaaactgg atgaggtggc ctatattcgt tttgcctcgg tctaccgtcg ttttgcggat
     2701 gtgagccaat ttctcaaggc ggttcaagat atggtcgagt cgtagccccg gccgtcagat
     2761 atgctgaaat taaccgtata tattccggaa acgcatctgg agtcggtgaa agcggcgctc
     2821 ttcgccgctg gagcgggacg ttacgccgca tatgaccact gttgctggca ggtgctgggc
     2881 gagggacagt tccgtccgct tgcgggcagt tcaccgtata tcgggaatac aggctccgtg
     2941 acgcgcgtag ccgaatatcg ggtggagatg atctgcgcgg aagcggagtg tgcggcggtc
     3001 acggcggcgt tacgcgccgc gcatccgtat gaggagccgg catttgattt tgtgaggctc
     3061 tgcgaagggt ccggatgagg ggaacggcat gaccatccgc gagcatctgg atgaggcggt
     3121 ttgtttggcg cccgaggcac gtccacgatg aaagtccggc gggtggctta tgccgggacg
     3181 cttgatgagc cggtctctcc ggtaacgtca aacgccgacg gcgcggagat gatgctctga
     3241 cgccagtgcc agagcacccc gagggaaaaa agcgtcagtc ccgcaacccg caccgggatc
     3301 gcgtaccatc cgtgcaccag tccaagcagg ttctgctgtg aggcgagcgc gaccaacagc
     3361 aggacgccga gccatacgct ccggtggcgg aggacaatcg ggatgccgag catgaccggc
     3421 agcaggtaat agatgtccgc ataggccgcg cggtagggga tggcgtattc aaccagattc
     3481 acggccacga tgcccacgta ggcgagatag gcgaacggcc aaggcgtacg ggagcgggta
     3541 tagagggcgg caaggataag tgcgacagcc gtactgacgc cgacaagttt ggccaggaaa
     3601 agcacgcttg tgacgggaat cgaacagcga tcaagcaacg gtttggagag acgcaccatt
     3661 cctgcggcgg tgctgtttat ggacggcaac atcgccaatc cttgtttccg cgactgctcg
     3721 aaatcttgga tcggcaaggg gtcgcggccg cgaagcaagt cggcaaaatg atccgtctca
     3781 aaatcagttg aggcgatttc atatttctcg gaaatccaaa tgaactggcg ccagccttcg
     3841 accccgaaca cggctagcga cgctcctccc atgaacaaac acgcgcaagc aatgcccgtc
     3901 agcatacgcc atcggcgttg cagggcgaac ggcaacgcga gaaatgcgac ggtggggcgg
     3961 attacgacgg tgagtccgag aaggagtcct gacaggaaag gacggttcga cttgatgtgc
     4021 gccaccagtg aactggcgat cagcagacag gtaaacacgt agtattgtcc ccgttcaacg
     4081 tgcagacgcc agaagggcgc acctgccgta aagaaaagaa tcagggagaa agtcagtgcg
     4141 cgatccctgt ttcgaggaat caaacgggtc atcaggcaaa ggacggacag gaacgccaac
     4201 cattcgagtg caccactcac atatcgcgcc gtcgtgaaat cgaaccaagt aaacggcata
     4261 taaaacaaca aaaggcaggg cgaattgcat ccgacaccgg gcgggcgcgg gactcctttg
     4321 aaatgggttt catgaaacgc tttgtcgata ccgttcaacc accagtctgc cgtggcgtgg
     4381 tagggttcta tacccaacaa gccgaatttt gcgccgacgt agcgttgcag gaaatcaatg
     4441 ccctcgtact gccagagatc gatgaccgaa accacgaggc ttgcgaacaa gacaaacaga
     4501 gcggacagga agaagacgcg aagccaaaaa ggaagagggg tcagtcggaa accgaacacg
     4561 gtggcacaca acgagtctgt cttgttgcat cttatggcca gaggcaaaga aggttgtggg
     4621 aaagcgcctt cgttcatggt tttcttcctg cgttttcaga tagttctcgc ggaaaacggt
     4681 gagatgaaag aagcttttct ttacagaatc gatactccaa ggcggtcgcc agacaaccga
     4741 agccgtattt tacgcttctt tggaaattga tcgaggaggc ttcctcaaaa taacgtgtcg
     4801 ggcaggagat ttcgccgatg agggctccgc tccagaggat ttgcgcgagc atctgcgcgt
     4861 caaacgcgaa atcatccatg ttcgcctgta aggggaggcg ctcgagaagc gaacgcgaaa
     4921 aagcgcggta tccggtatgg aactcagaca gttttgatcc gaaaagtaaa ttcatgaaca
     4981 gggtcagcgc tcgattggaa aaatatttcc aaaaaggcat tccccctttg agggcgtagc
     5041 cccccagaat gcgcgagccg agcacacacg gatagagacc ggacccgatc aggctcacca
     5101 tggctggaat cagtttggga gtgtattggt aatccgggtg caccatgaca acgatgtccg
     5161 cgccggactc gagggccagc cggtaacacg ttttctgatt cgccccgtat ccggcgtttt
     5221 tcgggtgtac atggactcgt accttcggca gggtcgcagc aagagtcacg gtctcgtccg
     5281 tactggcatc atccacaaga ataatctcat cgacaatccc ttggcacatg acttcatcat
     5341 aggttttctt gagtgttttc gcggcattat acgccggcat gacgacaacg atcttttttt
     5401 tggtcatctc atatccttct gtcttaagag ggtaaaaaac tccttccact ggacgagtcg
     5461 gctacacgat ctggatcaat cctgtttccg tgatacggga catctcctga tattcgtaat
     5521 ccgtgttgat ggcccaaaga tcatggcacg tacccttgca taggttctgg gccgccagca
     5581 accccaactc gcatgatggc tcctttgagc cgattccgcc gggcttcctc accacggaag
     5641 tcccgaaaat tcggatcgga tcacgaaaaa tagtataggc attgacggtg cggattgcta
     5701 atataaaaac aaaaggattt acaatgaaga agcgtttccg gttttcaggc ggttccagat
     5761 ggctcgaagg cctggggctc ctgttcgtta tgctcctgtt ctggggcagt atgctctatg
     5821 tcttctatcc tgttctgtcc cgagactggg tgatgctgtc gccggatttc ccgaagttct
     5881 atccgacctg cgcgcaagcc aaatggttgg aaatggtttt ggcgggccgc ctttcggtgg
     5941 tgccgatgca cgcgctaaaa tttctcgggc atccgctgat ctggcaggag ttctttttta
     6001 tgctctccac agggcttgcc ggagtcgcgg tggcctatta tctgcgcact cagcgcgttc
     6061 ctcttgtacc ggcttgcggc gggggattgt gtttcgcctt gtccgggtat tcgttcaccc
     6121 tgttctgtgc cgggcacggc ggatatttct acctgatctc ctgcggtctg ttcgcattcg
     6181 gccttgtgaa ccggtgtttc caaacccggg cgttctttta tttcgcttta ctcggtgccg
     6241 ttctgatgtg ggcggagatc gaacaaccgg atatttggtt gttgtttgtc atgctgttgg
     6301 ccgcatatac gctttggcga tcttaccggg agtggcggga gtcggggagc ggttcgttta
     6361 tctggcgcgt ggttccgcgg tttttcctga cccttgccgt catgcttctg atcggttttc
     6421 aacaaatccg ttcggctttc accgagtcgc tgatgaaccg caccctgcag atggaagaac
     6481 actcttctgt cacggagcag gaacaaaaga cggaccctgt gcaagcggag gctcggaaac
     6541 gcgaagaacg ctggattttt gcgaccaact ggagtctgcc cccggaggac atgctcgaat
     6601 tcatcatccc cagtctgttc gggaatgact ccttccacat gccgcatccc tattggggga
     6661 ggctgggacg tccgcatgcg tccgtgtttc aaaaagggcg catgatgccg aactaccggc
     6721 agcatgtagt gtatctgggg gtggtggcgg ttctgcttgc gatctttgcg ctgttcgcgt
     6781 ggtgcgccgc ccgtcggcag gaggaaacgg ggaatcaaag aacggatgat gcgggcggcc
     6841 cgacgggaca ggggaggcag gccacaaggg tgtcggtctt tgccgacgtg ccgttctggg
     6901 tcggggtttg gggggtctgc gcgatgatct ctctgggacg gtacacgccg ttctataggc
     6961 tggtctatgc tttgcccttc atggaatatt tgcgcgcgcc tgtcaaattt catcatctgg
     7021 tggaactggc gaacgcctgt ctggccgggt ggggacttta tgcgctctcg agttcggatg
     7081 tctcgacacg gcggctccgg caacggtttg ccgtgttcgc ggcaggtgtg gcaggatgtc
     7141 tggtcgtttt ggcgttggtt cttcaggtcg gggctgcggg aatggaacgg catatcacgg
     7201 agttggggat ggggcaagtt gcgtccgctt tacgagggtt tgcggtagcc aacacggtcc
     7261 ggtccgcgct cctgacgctt gccgttgctg gtgttttcgc cgggtctgcc tggcgcggtg
     7321 tctccttaag gcgaagtctg ttctgcgtct gtttgctgac cgcattgaac acggccgatt
     7381 tggctgtcgt cgcgcgacgt tgcgtcaccc cggtgaatgt cgggccgcat catgccgcga
     7441 atctcgtggt aaagacgatg ctgtcgcgta ccgggggacg acccgcgaac gtcctcaact
     7501 atgtcacctc caacgtgtgg gatcaggatt ggtttcacac ggcgctggaa ttgcacggat
     7561 tcgcctgcgt ggggcctgat ccgcagaacc ggagcgcgcc gcgctcgctg ttgtttgagg
     7621 cgtttcagcg ttctcccctg cgatactggc aggtcatggg ggcacggttc gtgctgttgc
     7681 cgcgaaaaca cgctgaaccg tttttccgcc agaacatcct taccgctgta tgtgattttg
     7741 agatcgggcg tgacacggtc cggacaagtc agcccacgga aacgagtctg gtgctggcgg
     7801 aggtgaaggg tctgcccgcc ttgccggcat tgtacttcga gtgggagggt ccggtagcgg
     7861 gtgatcaaca actgcaggcg gtacgcgcaa aggagtcggt cgggcatctg gtggccgatg
     7921 tggatggggt gcggggtgtg gcgacgggga gtgcggtggc gccacgcagt gtcgtgttgg
     7981 acaccctgcg gaagatgccg cgtgcgctgg agacgcgcgg tctggttgaa acggaccgtc
     8041 ctgccctgct ggtgttaggg gagacgtatg acgcgggatt ggaggccctt gtcgaccgcg
     8101 tgccggtacg tgtgtgtcaa gccaacggga tgtgggccgc agttcccgtg cctgccggca
     8161 aacatgagat tgtggtgcgc atgaaacgga actgggggtt gaatgccctc agtgtggcga
     8221 cgagtctgac acttctcggc tggtttgcgg tacgtctcgc gcgaaggaat gtggggcggc
     8281 gacgcgcgac gtaatcggcg cgaaggcgga gggagggttg tgggcgaagg cgatcgttat
     8341 tattcgaatg ccagaccgga aatggcccgc ttcatccatg ggaggccttc ccggattctg
     8401 gagattggat gtgcgggcgg atgtttccgt tcgaacatct tttggccatg cgaatattgg
     8461 gggggggagc cggtacctga tgtggcggcg cgcgcgtccc aaaccctgtc gcgtgtcctg
     8521 accggaacgt atgaggctgt cgcagccgaa ctgccggagg gatattttga cctgattgtc
     8581 tgtaacgacg tcatcgagca tatgctggat ccggatgctt ttcttcgttc ggtccgaacc
     8641 cggctcaatc cgcagggtca gttagtcggt tcggtgccga acgtacgcta tctgttgaat
     8701 ctgatcaatc tgctgattat gaaagagtgg cggtatgagg cgggaggcgt gctggatgcg
     8761 acgcacctgc gttttttcac catgaagagc cttgcgcggc tgttacggga aaccggtttt
     8821 cggattgagc ggttacaacc ggtcggcccc gaccggtatt tacggctcaa acggtgtttg
     8881 gaaattgtgg cttggtggat gggcacggat gtgctatatg cgcaaatggg gttcagatgt
     8941 cggatttgaa tacacaaaac gggaaagcgg gagcggttct ccacaaggat cgattcgctc
     9001 gggtgagtta tctggtggtg gggtctggat tttacggggc aaccatggcg gagcggatcg
     9061 cctccgtctt aggcgaacgg gtgatggtga tcgaaaagcg tgcggtggtg gggggaaaca
     9121 gtttttcccg tgcggacgag gaaaccggaa tcgaaatcca tctgcacgga tcgcatattt
     9181 tccatacgcg cgattctcgt atctgggagt atgtgcgatc tttcggcgcc ttcaatgcgt
     9241 accggcatca tgtgctggct tcgcatcacg gacgtgtgta cccgatgccg gtgtgcttgt
     9301 ggacgttaaa tcagtttttc ggtcgggtct tc
//
LOCUS       DAOSLE010000108         9225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7139_length_9225_cov_7.718321, whole genome shotgun sequence.
ACCESSION   DAOSLE010000108 DAOSLE010000000
VERSION     DAOSLE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7139_length_9225_cov_7.718321"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..915
                     /gene="rsxA"
                     /locus_tag="PK576_07865"
     CDS             337..915
                     /gene="rsxA"
                     /locus_tag="PK576_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOE61546.1"
                     /translation="MEYLLLLLSAILVNNFVLSRFLGICPFLGVSKRISTALGMSGAV
                     IFVITIASAVTWCLQTYVLAPLGLDKFLQTLAFILVIAALVQLIDIVMKRFTPSLHAA
                     LGIFLPLITTNCAVLGVAVLNMKLGYGLFKSVLFGFAAATGFAFALLIFTGLREKIDR
                     ADPPRCFRGVAISLVTAGLLSLAFMGFNGLVK"
     gene            935..1732
                     /locus_tag="PK576_07870"
     CDS             935..1732
                     /locus_tag="PK576_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOE61547.1"
                     /translation="MDLITTSTLCVGGIGLASAAALAAAEKYFTVKQDPRIDQVTELL
                     PGANCGGCGYAGCADYARAIVDGTAECNLCAPGGPDCAQAIAALLGRTVAVLEKRVAI
                     VLCCGDNTEATRRATYNGIADCASAQTIAGGDKACTYGCLGYGSCARICPVNAIHMLN
                     GIAKIDKKRCIACGKCVAACPRRLIKLVPAAAEIHVLCSSKDKGPAVKKVCGTGCLGC
                     SICAKLSDGAISMDGFLAMVDYSQPLTHEEVVAKCPGHCIRKDPPNS"
     gene            1764..3107
                     /gene="rsxC"
                     /locus_tag="PK576_07875"
     CDS             1764..3107
                     /gene="rsxC"
                     /locus_tag="PK576_07875"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOE61548.1"
                     /translation="MPTIPSSFTFHGGIHPDYHKELAAGSPIRELPLPARLVVPLSQH
                     LGASAKPVVQTGSTVIGGQLLAEADGLISAPVHAPAAGKVVALEPTLTATGRACDAIV
                     IETASEQTWESLPPLPEWRTADPKSLIERIGVAGVVGMGGAGFPTRVKLSPPRDKPID
                     TVILNGAECEPYLTADHRMMLEHAADIRLGAEIICHILGAKTLRVAIEDNKPDAIEVL
                     EKIFQDMDGDVAVTVLRTRYPQGSEKQQIFSVTGREVPRGGLPMDVGCVVENVSTAFA
                     IAEAVARGRPLVRRVITVTGDAIAQPANLLVPCGALYSDLVAACGGITGKVSKVISGG
                     PMMGFTVSNLAVPVGKTASGLLLLSPKNTTCFTSQACIACGRCVDACPMRLSPAELSQ
                     CIEAEDIEAAEPLAVMDCIECGSCAFVCPAHRPLVHHMRRAKAHIMARRAAAKTS"
     gene            3139..4203
                     /locus_tag="PK576_07880"
     CDS             3139..4203
                     /locus_tag="PK576_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOE61549.1"
                     /translation="MKPEASPDLFIISPSPHIHAGSSVRRIMLDVLIALLPALAASLW
                     FFRWHALRLTVTCVATCLLTEWLCRKAMRRASTLSDLSAAVTGLLLAFNLPPALPTWM
                     AVAGSVFAITIAKQVFGGLGYNPFNPALAARAFLLISFTGAMTSWSGSPWIRKVTYAA
                     SEATTKATSALAADALTSATPLGLAKEALKSGQGMPLFSDPTLLRDLFLGNINGCIGE
                     TSALALLIGAAYLLFRRVITWHIPGAFLASVLVYAGILHAVSPAASLPPHVHLLSGGL
                     LLGAFFMATDMVTSPLTRRGMLIFGAGCGLLTMLIRTVKTGAYPEGVSFAILIMNAFT
                     PLINRATRNRVFGTQPHPKP"
     gene            4200..4763
                     /locus_tag="PK576_07885"
     CDS             4200..4763
                     /locus_tag="PK576_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOE61550.1"
                     /translation="MNDTIRLILVLTVICSISSALLAAVYRQTQAPIEATLRQRTLRT
                     AATVLPCGSDTPTPVELDGTTFYVARQGGAFAGAALEGHSANGYGGDMVLMVGLSADG
                     KLVNFKKLVGSETPGLGTKIDTDLFRKPLLGRSLKANWTVRKDGGEVDAVTAATISSR
                     AALECIRDAIARFEKALPSLTAAPSDR"
     gene            4887..5198
                     /locus_tag="PK576_07890"
     CDS             4887..5198
                     /locus_tag="PK576_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61551.1"
                     /translation="MKETRHSKTQSAHARAVLFLCERLTNRGHTVISANTDEKADPQI
                     FAQSETGELAFYFVRTGTSTPPEDDLARFRALAAHHRVAAYFAPVTFSPAPHCAEVRS
                     L"
     gene            5523..7076
                     /locus_tag="PK576_07895"
     CDS             5523..7076
                     /locus_tag="PK576_07895"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOE61552.1"
                     /translation="MSEQTQTEMRRIYMFDTTLRDGEQSPGCTMSLRDKLLVAEHLEA
                     LRVDIIEGGFAIASPGDFEAVKSIAGVVKNAAVASLCRALPKDIDRTWEAIRSARHPR
                     IHTFIATSPIHMEFKLNMTPDQVYDQAVAMVRYARNLCGDVEFSLEDCSRSERPFIYR
                     IIEGVIAAGAGTINIPDTVGYAVPEQFGQLIADIRVNVPNVDKARLSVHCHNDLGLAV
                     ANSLAAIRAGADQVEGTINGIGERAGNTALEEVVMALHTRREFFQAQTGIDTTKIYAT
                     SRCVATVTGSRVQPNKAIVGENAFAHEAGIHQHGVMANPLTYEIMTPESIGLPRNKMV
                     LGKHSGRHAFESRLRELGFPLDAERMQELFVRFKDLADRKKNISDADLEALAQDVQSM
                     VTEHVKLDRFNVTASNAITPVSTIRLLRDGKLVEQVAASDGPINASFKAINQILGLKV
                     TLESFQLTAITGGTDAQCEATVRVRDGDHIYHGRGVSTDVIEGSILAYLQALNSLLDE
                     AAETSKRPA"
     gene            7205..7280
                     /locus_tag="PK576_07900"
     tRNA            7205..7280
                     /locus_tag="PK576_07900"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7238..7240,aa:Thr,seq:tgt)
     gene            7330..8994
                     /gene="ilvD"
                     /locus_tag="PK576_07905"
     CDS             7330..8994
                     /gene="ilvD"
                     /locus_tag="PK576_07905"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE61553.1"
                     /translation="MTSDQVKTGFQRAPHRSLLMATGVPRQDFKKPFIAVCNAYTDII
                     PGHCHLNQVGEWVKECIREAGGVPFEFNTIGICDGIAMGHTGMKYSLPSRELIADGVE
                     SMVRGHCFDAMICLPNCDKITPGMILGALRCNIPTLFASGGPMRAGRLDGKAIDLISV
                     FEGVAQFNNGKIDEKQLEAIESSACPGCGSCSGMFTANSMNCLCEAIGLALPGNGTIL
                     ADDPARRELWKRAAQRVVEMALAGGPRPRELITLEAVDNAFALDMAMGGSSNTVLHTL
                     AIAREAGLDYDLDRINAISQRCPNVCKVAPSCAYHMEDVDAAGGIPAILKRIADIPDL
                     LDTTCPTVSGLTIGEIIAAAEIKDAEVIRTLDNAYSKDGGLTILRGNLAEQGAVVKKA
                     GVAASMLTFEGRAICFDSQEEACEGILDGKVKAGHVVVIRYEGPRGGPGMQEMLAPTS
                     YIMGAGLGESVALITDGRFSGGTRGACIGHISPEAAEGGLIGLLRDGDPIRIDIPGRR
                     LEALLDEAEIARRRAAAPAWKPRDLGGWLRRYALSVGNASSGASLL"
BASE COUNT         1751 a         3094 c         2678 g         1702 t
ORIGIN      
        1 gtgccggaaa cgcgggcgtc cctgtcgatc gtcgcgggtc gaatgtcgcg ggtcgacgat
       61 cccgataccg atcccgatac cgatcccgat accgatcccg atttggatga aaccctgaaa
      121 ccatgaaacc cagaaacctt gaaaccctga aaccttgaaa ccctgaaacc cagaaaccct
      181 gaaaccctga aaccctgaaa ccagaaacct tgaaaccttg aaaccctgaa accagaaacc
      241 cttgtaattt tgtagttgcc gagtcgcacg cagagattga accccatacc gggatttgat
      301 atttttgggc tttgttcctc acctgaggat gtggacatgg aataccttct gttacttctc
      361 agcgcgattc tggtcaacaa ctttgtgctg agccgttttc tcggcatctg tccctttctg
      421 ggcgtttcca agcggatcag caccgcgctc ggcatgtcgg gcgcggtgat cttcgtgatc
      481 accatcgcgt cggccgtcac ctggtgcctg caaacctatg tgctggcccc gctgggactg
      541 gacaaattct tacagaccct cgctttcatc ctggtgatcg ccgcgctggt gcagttgatc
      601 gacatcgtga tgaaacgttt caccccgtcg ctgcatgccg cgctcggcat cttcctgccg
      661 cttatcacca ccaactgcgc ggtgctcggc gtggccgtgc tgaacatgaa actgggatac
      721 ggcctcttca aatccgtgct gttcggcttt gccgccgcaa ccgggttcgc ctttgccctg
      781 ctgattttca ccggtctgcg cgagaaaatc gaccgcgccg acccgccccg ctgtttccgc
      841 ggggtggcca tctccctcgt caccgccgga ctcctgagcc tggcattcat gggcttcaac
      901 ggcctcgtca aataaccctt ctgaaaaccc tttcatggat ctcatcacca cttcaacctt
      961 gtgtgtcggc ggcatcgggc tcgcgagtgc cgcagcactg gctgccgctg aaaaatactt
     1021 caccgtcaaa caggacccgc gcatcgatca ggtgaccgaa ctgctgccgg gcgccaattg
     1081 cggcggctgc ggttacgcgg gctgtgccga ctacgcgcgc gcgattgtgg acggcaccgc
     1141 ggaatgcaac ctctgcgcac ccggcggccc ggactgcgca caagcgatcg cggctttgct
     1201 cggtcgcacc gtggccgtcc tggagaagcg ggtcgcgatc gtgctctgtt gcggcgacaa
     1261 cacggaagcg acacgccgcg caacctacaa cgggatcgcg gattgtgcct ccgcccagac
     1321 gatcgcgggc ggagacaagg cttgcacgta cggatgcctc ggatacggtt cctgcgcccg
     1381 aatctgcccg gtaaacgcga tccatatgct caacggcatc gccaagatcg acaagaagcg
     1441 ctgcatcgcc tgcggcaagt gcgtcgcggc ctgtccgcgc cgactgatca agcttgtgcc
     1501 cgccgccgca gagatccatg tgctctgcag ttcgaaggac aaaggccccg ccgtgaagaa
     1561 ggtctgcggc acaggttgcc tcggttgcag catctgcgcc aaactctccg acggcgcgat
     1621 cagcatggac ggcttcctcg cgatggtgga ctactcccag cccctcaccc atgaggaggt
     1681 tgtcgctaaa tgtccgggcc actgcatccg caaagaccct cccaactcct aactcccaac
     1741 ccccacactc ctcccttctt tccatgccca cgatcccttc atccttcacc ttccacggcg
     1801 gcatccatcc cgattaccac aaggaactcg ccgccggttc gcccatccgc gaattgccgc
     1861 ttcccgcgcg gctggtcgtg cctctgtcgc agcacctcgg cgcgtcggcc aagcctgttg
     1921 tccagaccgg cagcacggtt ataggcggac aactcctcgc cgaagccgac ggtctcatct
     1981 ccgcgccggt ccacgcgccg gccgccggca aggtggtcgc gctcgaacca acgctcaccg
     2041 cgacaggccg cgcctgtgat gccatcgtga tcgaaaccgc gtccgagcag acgtgggagt
     2101 cgttgccgcc cctcccggag tggcggacgg ccgaccccaa atcgctgatt gagcgcatcg
     2161 gtgtcgccgg agtcgtcggc atgggtggcg caggcttccc cacacgcgtg aaactctccc
     2221 cgccgcgcga caaacccatc gacaccgtga tcctcaacgg cgccgagtgc gagccctatc
     2281 tgactgcgga tcaccgtatg atgctggaac acgccgcgga catccgcctc ggcgcggaga
     2341 tcatctgcca catcctcggc gcgaaaaccc tgcgcgtcgc aatcgaggac aacaaacccg
     2401 acgccatcga agttttggaa aaaatctttc aggacatgga tggcgatgtg gccgtgaccg
     2461 tgctccgaac ccgttacccg cagggctcgg aaaagcaaca gatcttttcg gtcaccgggc
     2521 gcgaggtgcc gcgcggcggt ctgccgatgg atgtcgggtg tgtggtggag aatgtcagca
     2581 ccgccttcgc gatcgccgaa gccgtggccc gcgggcggcc gctggtccgg cgcgtgatca
     2641 cggtcaccgg ggacgccatc gcgcaacccg cgaatctgct cgtgccctgc ggcgcactgt
     2701 acagtgactt ggtggccgcc tgcggcggga tcacgggcaa agtgtccaag gtgatctcag
     2761 gcgggccgat gatgggcttc acggtcagca acctcgcggt accggtcggc aaaaccgcct
     2821 cgggcctgct gctgctctcc cccaaaaaca ccacctgctt cacctcccaa gcctgcatcg
     2881 cgtgcggacg ctgcgtggac gcctgcccca tgcgcctgag cccggcggaa ctcagccagt
     2941 gcatcgaggc ggaggatatc gaagcggcag aacccctcgc cgtgatggat tgcatcgagt
     3001 gcggctcgtg cgcctttgtc tgtcccgccc atcgtccgct ggtccatcac atgcgacgcg
     3061 ccaaagccca tatcatggca cgccgcgctg ccgccaaaac ctcctaacct gaaacccgca
     3121 acccgaaacc ccttcccaat gaaacccgaa gcttctccag accttttcat catcagccct
     3181 tcgccgcata tccatgcggg ttcctccgtg aggcgcatca tgctggacgt gctgatcgcg
     3241 cttttaccgg ctctggcggc atcgctctgg tttttccgat ggcatgcgct gcgactcacc
     3301 gtaacctgtg tcgccacctg tctgctcacc gaatggctct gccggaaagc catgcgccgc
     3361 gcctcaaccc tctccgatct cagtgccgcg gtcaccggcc tgttgttagc gttcaacttg
     3421 ccgcctgctc tgccgacctg gatggcggtc gcgggctcgg tcttcgccat caccatcgcc
     3481 aagcaggtat tcggcggact cgggtacaac ccgttcaacc ccgccctcgc ggcccgcgcc
     3541 ttcctgctca tctcgttcac cggcgcgatg acctcctgga gcggctcccc ttggatccgc
     3601 aaagtgacct atgccgcgag cgaggccacc acgaaagcaa caagcgcact ggcagccgac
     3661 gccctgacct ccgccacgcc gctggggtta gcgaaggagg cgctcaaatc cggccagggc
     3721 atgccgctgt tcagcgaccc gacactgctc cgggatctct ttctgggcaa catcaatggc
     3781 tgcatcggcg aaacttcggc cttggccctg ctgatcggcg cggcctacct gctgttccgc
     3841 cgggtcatca cctggcatat ccccggtgcc tttctggctt cggtcctggt ctacgccggc
     3901 atcctgcatg cggtctcgcc cgccgcctcg ctcccgccgc acgtgcacct gctctccggc
     3961 ggcctgctgc tgggcgcgtt tttcatggcc accgatatgg tcacctcgcc gctcacccgg
     4021 cgcggcatgc tgatcttcgg cgcaggctgc ggcctcctca ccatgctgat ccgcaccgtg
     4081 aaaaccggcg cctatcccga aggggtgagc ttcgccatcc tcattatgaa cgccttcacg
     4141 ccgctcatca accgcgccac gcgtaaccgc gtgttcggca cccaacccca tccgaaacca
     4201 tgaacgacac gatccgactc attctggtgt tgaccgtcat ctgctcaatc agctcggctc
     4261 tgctggccgc ggtttaccgt caaacccaag cccccatcga agccactctt cggcaacgca
     4321 cgctccgcac cgcggccacc gtcctgccct gcggaagcga cacgcctacc ccggtggagt
     4381 tggacggcac cactttttac gtggcccgtc agggcggggc cttcgcgggt gccgcgctgg
     4441 aagggcactc ggccaatggt tacggcggcg acatggtgct gatggtcggt ctgagtgcgg
     4501 acggcaaact ggtgaatttt aagaaacttg tcggttccga gactccgggg ctcggcacca
     4561 aaatcgacac ggaccttttc cggaaaccac tgctgggccg gtccctcaaa gcgaactgga
     4621 ccgtccgcaa agacggcggc gaggtcgatg ccgtcactgc cgcgacgatc tcctcccgtg
     4681 cggcgctcga atgtatccgc gacgcgatcg cccgcttcga aaaggccttg ccgagtctaa
     4741 ccgccgcgcc gtccgaccgg tgagacgggc agacgctcgt ccctccccaa acaggtgcgg
     4801 cgcggatcgc gacggaagcc aatgtttacg aggctttgct cgaattctgc ccggacaaat
     4861 ccgccggagc ggcaggagag aacaacatga aggaaacgcg gcacagcaaa acgcaaagcg
     4921 ctcatgcgcg cgccgtcctc ttcctctgcg aacggttgac gaaccgtggc cataccgtga
     4981 tttccgccaa tacggacgag aaggcggacc cgcagatctt cgcgcaatcg gagaccggtg
     5041 aactggcctt ctattttgtg cgcacgggga cgtcgacgcc cccggaggat gatctcgcgc
     5101 ggttccgcgc gctggcggcg caccaccggg tcgccgccta tttcgcgccg gtcacgttct
     5161 cccccgcgcc gcactgtgcg gaagtgcgct cgctgtgacg tcccgtaaac cgctccgcag
     5221 cggacggcac acgggatccc cccaaaaaaa tgcgcggcgc ccctttttcg ccttgcacac
     5281 ccccccggtc ttatgacata gtgtgcgctg atgaatgaca aatccactgt ttgcttgttc
     5341 cgacttaacg gactggggct cttgcgggcc tgcaggaggg ttaccgtgta atcggatcgg
     5401 catacaggtg aacggtaagg actcccgcag gtaatctcaa acgattccgc gggagttttt
     5461 gtttttgtca caacgtacgg gccagcggca caccgcacag cataaaccaa cgaggaaaaa
     5521 ccatgagcga gcaaacgcaa acagagatgc gacgcatcta catgttcgac accaccctgc
     5581 gcgacggcga acaatccccg ggttgcacca tgagcctgcg cgacaaacta ctggtggcgg
     5641 aacacctcga agcgctgcga gtggatatca tcgaaggcgg cttcgcgatc gcctcgcccg
     5701 gtgattttga ggccgtcaaa tccattgcgg gcgtggtgaa gaacgccgcg gtcgccagcc
     5761 tgtgccgcgc gcttcccaag gacatcgacc ggacctggga ggcgatccgc agcgcccggc
     5821 acccgcggat ccacaccttc atcgcgacct cgccgatcca catggaattc aagctgaaca
     5881 tgacgccgga ccaggtctat gatcaagcgg tcgccatggt ccggtacgcg cggaacctgt
     5941 gcggcgatgt ggagttttcg ctggaagact gttcccgcag cgagcggccc ttcatctacc
     6001 ggatcatcga aggggtaatc gccgccggcg cagggacaat caatatcccc gacaccgtgg
     6061 ggtacgcggt gccggagcag ttcggccaac tgatcgcgga catccgcgtg aacgtgccca
     6121 acgtcgacaa ggcacggctg tctgtgcact gccataacga tctgggcctg gcggtcgcca
     6181 actcgctggc cgcgattcgg gccggcgccg atcaagtgga gggcacgatc aacggtattg
     6241 gcgagcgggc gggcaacacc gctctggaag aggtggtgat ggcgttgcac acgcggcgcg
     6301 agttttttca ggcgcagacc gggatcgaca ccacgaaaat ctatgcgacc agccgttgcg
     6361 tcgcgaccgt caccggcagc cgcgtgcagc cgaacaaggc gatcgtgggc gagaacgcct
     6421 tcgcgcacga ggcaggcatc caccagcatg gggtgatggc caacccgctg acctatgaga
     6481 tcatgacccc cgagtcgatc ggtctgccgc gcaacaaaat ggtgctcggc aaacactcgg
     6541 ggcgccacgc ctttgagagc cgcctgcgag aactgggctt cccgctcgat gcggagcgta
     6601 tgcaggaact gttcgtacgg ttcaaggatc tggcggaccg gaaaaaaaac atcagcgacg
     6661 ccgacctcga ggccttggcg caggatgtcc agagtatggt caccgagcac gtcaaactcg
     6721 accggttcaa tgtcacggcc agcaacgcga tcacgccggt cagcacgatc cggttgctgc
     6781 gtgacgggaa actggtcgag caggtcgccg caagtgacgg accgatcaac gcctctttca
     6841 aggcaatcaa ccagattctg gggctcaaag tcacgctgga gagcttccag ctcaccgcca
     6901 tcaccggcgg cacagacgcg cagtgcgagg ccactgtccg ggttcgtgac ggcgaccata
     6961 tctatcacgg acgcggcgtt tccaccgacg tcatcgaggg gagcatcctg gcgtatctgc
     7021 aagccctgaa cagcctgctg gatgaggctg cggaaacgtc caaacggccc gcgtaaccgg
     7081 cgcgcgcgcc cctttcgact tgaaacgtga accggtgtta tcgtaagatc ataccggtta
     7141 cgtttcaagg cgtacaacgc gcgcggcagg ttctttcccg tccgttgcgc aggccaccct
     7201 tttagccagc atagctcagt tggtagagca cttgatttgt aatcatgagg tcgtcggttc
     7261 gaacccgtct gctggctcca ttcaaaaaac gaccatagcg aagccgaata ataattaagg
     7321 aagaacacca tgaccagcga ccaagtcaaa accggctttc agcgtgcccc ccaccgtagc
     7381 ctcctcatgg ccacgggtgt gccgcgtcag gatttcaaaa agcctttcat cgctgtgtgc
     7441 aacgcctata cggacatcat tcccgggcac tgccatctga atcaggtggg cgaatgggtc
     7501 aaagaatgta tccgcgaagc gggtggcgtc ccgttcgagt ttaacaccat cggcatctgc
     7561 gacggtatcg ccatggggca cacaggcatg aagtattccc tgcccagccg cgaattgatt
     7621 gccgacggcg tcgagtccat ggtccggggc cattgcttcg acgccatgat ctgtttgccc
     7681 aactgtgaca agatcacccc cggcatgatc ctcggtgccc tccgctgcaa catccccacc
     7741 cttttcgcca gcggcggccc catgcgcgcg ggccgtcttg acggcaaagc catcgatctg
     7801 atctcggtgt tcgagggcgt cgcccagttc aacaacggca agatcgacga aaagcagctt
     7861 gaagccattg aatcgtcggc ctgtcccggc tgcggatcct gttccggcat gttcaccgcc
     7921 aactccatga actgcctttg tgaagccatc ggactggcgc ttcccggaaa cggcacgatt
     7981 ctggcagacg accccgcacg acgcgaactc tggaaacggg cggcgcaacg cgtcgtcgag
     8041 atggccctcg cgggggggcc gcgtccacgc gagttgatca ccctcgaagc cgttgacaac
     8101 gccttcgcgc tggacatggc gatgggcggc agttccaaca cggtgctgca cacactcgcc
     8161 attgcgcgcg aggcggggct cgactatgac ctcgaccgca tcaacgccat ctcccagcgt
     8221 tgccccaacg tctgtaaagt cgcgccttcc tgtgcctacc acatggagga tgtcgacgcc
     8281 gcgggcggca tcccggcgat tctcaagcgt atcgctgaca ttccggatct gcttgacacc
     8341 acctgcccga ccgtcagcgg cctgaccatc ggcgagatca tcgccgccgc cgagatcaaa
     8401 gacgcagagg tcatccgcac gctcgataac gcctactcga aggacggcgg tctcacgatc
     8461 ctgcgcggca atctcgccga gcagggtgca gtggtcaaaa aggccggtgt cgctgcctca
     8521 atgctcacct ttgaaggccg cgccatctgc ttcgactcgc aggaagaggc gtgcgaaggc
     8581 atcctcgacg gcaaggtcaa ggccggccat gtggtggtca tccgctacga gggtccgcgc
     8641 ggcggccccg gcatgcagga gatgctcgcg ccgacctcct acatcatggg tgcgggcctc
     8701 ggcgagtccg tggcgctgat cacggacggc cgcttctccg gcggtacgcg cggcgcctgc
     8761 atcgggcaca tctcccccga agcagccgaa ggcggtctga tcggtctgtt gcgcgatggc
     8821 gacccgatcc gcatcgacat tcccggacgg cgtctggagg cgttgctgga tgaagcggaa
     8881 atcgcgcgcc gccgcgccgc cgcaccggcc tggaaaccgc gtgacctcgg cggatggctg
     8941 cggcgttacg cgctgtcggt cggcaacgcc agttccggtg cctcactgct gtaatccggg
     9001 gttgcatagc atgccgacgg caagagcgtc tccccgatgc gtgcgtgacg gagtcggggt
     9061 cttgcccgct atcccgtttc gccaaagggt tcagcgcttg gccatcaaag ctttccgcgc
     9121 atcggcttga agtggcccgg actttcacct atactgcacg tcatctgttc cggaggggtg
     9181 tctgagtggt ttaaagaacc ggtcttgaaa accggcgtgc cgcaa
//
LOCUS       DAOSLE010000109         9194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7164_length_9194_cov_7.624029, whole genome shotgun sequence.
ACCESSION   DAOSLE010000109 DAOSLE010000000
VERSION     DAOSLE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_9194_cov_7.624029"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1183)
                     /locus_tag="PK576_07910"
     CDS             complement(155..1183)
                     /locus_tag="PK576_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61554.1"
                     /translation="MCTSMLLAAAVSVFTVENPKGYNSWPMIGETGGKLVCAYSKGLK
                     HAIGEGERGAYVRTSSDGGRTWGEEVCLANAPDLGEVPIGKGKDATGAALFWNRCCGS
                     TSRHDLYRTTDGKAFERLASLKLDPHPTQITDIFEVDGNLMSFWFRCSYGDKKGLNAW
                     GTVISKDGGRTWGQVLVESGLTPTELPTEQAGFYAGNGRVFAIARTEGGAGQFQLTSL
                     DSGKTWKKTRTNIKDVYCSTPSLIYDAKTDTIYNYYYQRGAGKLKRRVAFLADVFGHP
                     TRWPEPDVLVTDTPVPPAFDCGNANAVRLGDKHVISYYTGKYPDTGVFVCVVDVGKTE
                     VVARPLGF"
     gene            complement(1283..3217)
                     /locus_tag="PK576_07915"
     CDS             complement(1283..3217)
                     /locus_tag="PK576_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOE61555.1"
                     /translation="MSRFFCVTMMLIAGTAFTKQPPNNADMKVSSSGLWAIFGSFGKI
                     CGDAAVVPDGYRLEESGCRIETTVETDKGVSLRKTVLKNVSDKTITAYCLMDRFPIED
                     GEWEVYTQVNYWENESQGQWQPLHTAVEVRNPNLRSSHGAAPMLAVWNRQAGRGRVFH
                     LLSDSAWELHAVCSNPGGNRTSFAVEAGMDSRQLAYSLAPGEHVALPEVLSYEFENRL
                     DLDCHKLHAWWNRHHPGRPPAAIYNTWLCRFDKLDADFLLKQVARAKDLGMEYFVIDA
                     GWFGPAAGWASVRGEWAERPDGHLKGRMKEISAAARKADLKFGFWVEAESVTGETRVA
                     REHPEWLLPANRSFLDFTNPDARRYITETVCALVKEYDASFLKFDFNQNLDHDPTGRA
                     FAAYNAAYRAFVRDMRRRNPGIYIEGCASGGYMMDLGWARDFDGFWLSDNQSIGHGLR
                     IVRETMLRFPPRQIERWLTVRSLTDIQPDYGGGDERLVSTEDAVWRNLRSYPLESAAA
                     FTAGGPLCFSCDLTALSENHFAYFQKLVAAHRQDAAFWARAVGRVLCAESSVQAVQYS
                     DEALTDVRIFVTADSSTQRTVTVTPVVDPSARYVRDDGHESTGAALRASGVSVALDRL
                     AAKEIRLRKCMGSSVTSVKK"
     gene            complement(4003..5280)
                     /locus_tag="PK576_07920"
     CDS             complement(4003..5280)
                     /locus_tag="PK576_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006849405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE61556.1"
                     /translation="MRAYKDRVVDSLLRRKLEGTGAVLIEGPKWCGKTTTAEQVAQSV
                     IYMAEPQKRDDNIAMARMDPGRILSGARPRLIDEWQIAPVLWDAVRHDVDHAGDLGRF
                     ILTGSSVPANEDEIKHSGTGRFAWLKMRPMSLWESEESSGEISFRELFSGANVQTASA
                     KPLELEEIARIVCRGGWPQAVSLSGNAALDQAYNYVDAITKKDISRVDGTTRDEDRAR
                     RLMRSYARLQGTQASASVIKEYLSTNEMTSIDNGTIYAYLNALRRLFVVEDMPAWNPN
                     LRAKDSIRTSDARYFVDPSIAAAALEIGPEDLMNDLTTFGLLFETMAVRDLRVYADAL
                     GGTVRHYRDRHGLECDAVVHLRNGAYGLIEIKLGGDKPLQAGTETLTKLARLIDSKKV
                     RKPDFQMLLTAVGSFAYRRDDGILVCPLSALRP"
     gene            5687..6337
                     /locus_tag="PK576_07925"
     CDS             5687..6337
                     /locus_tag="PK576_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOE61557.1"
                     /translation="MSNLSPTPGSSRIVKHLRTRLVSGLLVLIPLAITLFVLNIFLNS
                     LTAFARPLLKPLLGVLPESVLTLIALTLTVLLIYLTGLVTTHIVGRRLILFGERLLLR
                     LPVVKTIYAASKQVVETFSSHQTTSFQAVVIVSFPRRESLAIGFITGTLFAPDNRKLY
                     RVFIPTTPNPTSGFLLLMSPEDVQFTDIPIEEGIKMIVSGGMLAPEKYAVVPDNCP"
     gene            6396..7037
                     /locus_tag="PK576_07930"
     CDS             6396..7037
                     /locus_tag="PK576_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61558.1"
                     /translation="MAKPRREVEITLHSIRVPVTGTGLDTDTSHLLTATLIWPRLGVA
                     GKSASQPCALQHGESSFETVHWGRRILFKEAVEGRFALGLVLTEALDDEEMEKFLRFW
                     TGAFLAVGADLAGAAAPAPAGKLAAAPLDYAAKAIAKYPGALPLVEGLVEINAAELPP
                     GGRERLLTVRLTAARTLARLSRRTVNSRPRVSRSLLLEKGADNGEVTLAIRSL"
     gene            complement(7039..>9194)
                     /gene="secD"
                     /locus_tag="PK576_07935"
     CDS             complement(7039..>9194)
                     /gene="secD"
                     /locus_tag="PK576_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOE61559.1"
                     /translation="KSIQSAAFLEFKLVHKQNEQLVDKVLASGRLPEGYVSSDDGRGY
                     KRAPNYTELVKDPEYMHRLSMFEVPDPRYAFMLEREQTANGQVIYRPIYVLRKAEMTG
                     DALSSASVEIEPMTGRVHVSLVFNAKGGADFARLTKAYAPRGSRNKDSDVGRRLAIVL
                     DDTLYSAPVLKTEIPNGRAVIEGNFSWSEAAVLRNILNAGSLPAPMKILEKRSVESTL
                     GADAIRSGIQASILATVLVALFMLVYYAYCGLIANVALLLDLLLLPAGLIMASSILGV
                     FVKDTAIAKRVLDLPVLTMPGIAGIVLSLGMAVDANVLIFERMREEFALGKSARAAVA
                     AGYDRAFLAIFDSNITTLLTAVILFIFGAGPIRGFAITLTAGIIISMFTALVVTRLIF
                     NATVPENRTRPYKMLQFMKNSNVDFLRWGKPTGYTSLAIIVVTLAIFFARGLKDPASV
                     MAVDFTGGVSMTFTYEQKADIGTVRKAVTDAVKNDATIQYQSTLDGSGNLLLVKTSVT
                     QIGEESASKVIQKALAKELPESRFTLAGEEDVGSEIGGDLKLSAAKAILFSLIGILIY
                     VSLRFEFGFALGGVVALAHDAFITLGLYSLFGRQVSLTVVAALLTIVGYSINDTIVIF
                     DRIREDMRKDVKMPFPELCNRAINQTLSRTILTSLSTLIATVTLFIFGGGSINDFALA
                     MMIGLIAGTYSTIFIATPVMLAWYRGRRPAFVTAK"
BASE COUNT         1964 a         2885 c         2493 g         1852 t
ORIGIN      
        1 ccgaccccga tttggatgaa accctgaaac cagaaaccct gaaaccctga aacccagaaa
       61 cctgaaaccc tgaaaccaga aacccctgaa acccaagagc gttgaaggtt caactgctct
      121 ttttaggttt aaccttatgt ttcgcaaacg agcctcaaaa tccaagggga cgtgcgacaa
      181 cctccgtttt accgacatcc acgacacaga caaaaacccc ggtgtcggga tatttgccgg
      241 tgtaatagga aatgacgtgc ttgtcgccaa ggcggacggc attggcgtta ccgcagtcaa
      301 acgcgggcgg caccggagtg tccgtcacga ggacgtccgg ctccggccag cgtgtcgggt
      361 ggccgaaaac gtctgcgaga aaagcgacgc gacgcttgag ctttccggct ccgcgctggt
      421 agtagtagtt atagatcgtg tccgtcttcg catcgtatat gagcgagggt gtcgaacagt
      481 agacgtcttt gatgttcgtg cgcgttttct tccaggtctt tccgctgtct aaactcgtca
      541 gctggaactg tcccgcgccc ccctcggtac gggcgatcgc gaaaacgcgc ccgttcccag
      601 cgtaaaagcc tgcctgttcg gtcggcagtt ccgtcggggt gagcccgctt tcgacaagaa
      661 cctgtcccca cgtccggcca ccgtctttcg agatgaccgt tccccaggca ttgagtcctt
      721 ttttgtcgcc gtagctacaa cggaaccaga aggacatgag gttgccatca acctcgaaga
      781 tatccgtgat ctgcgtcggg tgcggatcga gcttcagcga ggcgagacgt tcaaaggctt
      841 tcccgtccgt cgtgcggtag agatcatggc gcgacgtgct tccgcagcaa cgattccaga
      901 aaagtgccgc ccccgtcgcg tccttgccct ttccgatcgg cacctcgccg agatcgggcg
      961 cattggcgag gcagacttcc tcaccccacg tccgtccgcc gtcggacgag gtgcgcacgt
     1021 aagcaccgcg ctcgccttca ccgatagcgt gcttgaggcc cttcgagtag gcacagacga
     1081 gcttgccgcc cgtctcgccg atcatcggcc atgagttgta accctttgga ttctccacgg
     1141 tgaatacgga tacggccgcc gccaagagca ttgaagtgca catcaaaaaa accctccttg
     1201 gctaatcagt tgttttcgaa tagtgatttt actttacctc tttcatgaag aactcgctcg
     1261 ttgaatatcc gtaaaaccac gattactttt ttacggaggt tacggatgaa cccatacact
     1321 tccgaagtcg gatttctttt gcagccagtc tgtccaaggc aaccgacacg ccggaggccc
     1381 tcagcgcagc gccagttgac tcatgcccgt cgtcgcggac atagcgtgcc gatggatcca
     1441 caacaggcgt tacggtgacg gtccgctgag tcgaactgtc ggccgtgacg aatatccgca
     1501 catccgtgag ggcttcgtca ctatactgga cagcttgaac cgacgattcc gcgcagagca
     1561 cgcggccgac agcccgcgcc cagaatgccg catcctgacg atgcgcggca acgagcttct
     1621 ggaaataagc gaagtggttt tctgaaagcg ccgtaagatc acaactgaag caaagcggac
     1681 ctcctgccgt gaaagccgca gcactttcca gcggatagga ccggagattc cgccaaacag
     1741 cgtcctctgt cgagacgagc cgctcatcgc cgccgccgta atccggctga atgtccgtta
     1801 gcgaccgtac cgtcaaccat cgttcgatct ggcgcggcgg gaagcgcagc atcgtctcac
     1861 gtacaatgcg caggccgtga ccaatgctct ggttatcgct cagccagaac ccgtcgaagt
     1921 cgcgcgccca gccaagatcc atcatatacc ccccactcgc acaaccctcg atatagatgc
     1981 ccgggttacg gcggcgcata tcgcgcacga aagcacgata ggcagcgttg taggcagcaa
     2041 aggcgcgtcc cgtggggtca tggtcgagat tctggttgaa gtcaaacttg aggaacgatg
     2101 cgtcgtactc cttcacgagc gcgcacaccg tctccgtgat gtagcgacgg gcgtcaggat
     2161 tcgtgaaatc gaggaacgag cggttcgcag ggagcaacca ttcgggatgt tcacgtgcga
     2221 cgcgggtctc ccccgtcaca ctctccgcct ccacccagaa cccgaacttc agatccgcct
     2281 tgcgcgcggc agcggaaatc tccttcatgc gccccttgag gtggccgtcg ggacgctccg
     2341 cccactcgcc gcgcacacta gcccagccag ccgcagggcc aaaccatccg gcatcgatca
     2401 caaagtactc catgccgagg tctttggcgc gggcgacctg cttcagcaga aagtccgcgt
     2461 cgagtttgtc gaaccggcag agccaggtgt tgtagattgc cgctggcggc cgccccggat
     2521 gatggcggtt ccaccatgcg tggagcttgt ggcaatcgag gtcgaggcgg ttctcgaatt
     2581 cgtacgagag cacctccggc aacgccacgt gctcgcccgg cgcgagcgaa taggcgagct
     2641 gacgcgaatc catgcccgcc tctacggcga aggacgtccg gttgccgccc ggattggagc
     2701 agacggcatg cagttcccaa gctgaatctg agagcagatg gaagacacgt ccgcgtcccg
     2761 cctgccggtt ccacaccgcc aacatcggcg ccgcaccgtg ggaagagcgc agattcgggt
     2821 tacgcacttc aaccgcagtg tgaaggggtt gccactgccc ctgactctca ttctcccagt
     2881 agttgacctg cgtatagacc tcccactcgc catcctcgat cgggaagcga tccatcagac
     2941 aatatgcggt gatggttttg tccgaaacgt ttttgagcac ggtcttccgc agactcacgc
     3001 ccttatccgt ctcgaccgtc gtttcgatcc ggcagccgct ctcctcaaga cggtacccgt
     3061 ccggcacaac cgccgcatcg ccacaaatct tgccgaaact accgaaaatc gcccagagtc
     3121 cactggatga gaccttcata tcggcattgt tcggcggttg cttcgtaaat gccgtgccag
     3181 caatcaacat catcgtcaca caaaaaaacc gactcatttc atgatccctt ttataaaacg
     3241 taatctacaa gctccccaca cggaagtcaa cggcttgtat cctcacccaa attttccccg
     3301 ctccccccct cgcacttgaa ttgcatcaac cctcaccccc ctgtcacgaa ccgattacac
     3361 gttcaacgct caagtaagtg cgcaaaattt ggagtgcggt gacaagagcc gcgctgtgcg
     3421 cggagacaca caccaagtcc tctcgccaga cttgcggtca cgccgctaca ccgtaaaaac
     3481 ctttgcgtct ccgcgtctct gcgttaaaaa actttcaact ctcaacgccc aacttttttg
     3541 cgggcgagga cgcccgcgct cccgattgcg catacacaaa ccacgttctc aactcgcatg
     3601 ccccctgtca ccaaccgatt acatgaccac caataaacct gttgcacgcg cgtccctgag
     3661 tggtgcataa tgttatgcat aaatgaatgc gcatgatgtt ctcctttggt gatggggaac
     3721 gtcccgccgg ggacggctcc agggtacgga ttcccgttcg tccggcatgc gttctgaaat
     3781 gatctccttg cctataaatt gacaggatga caggattttc aggattgaac aacatcatgt
     3841 taatcatgga tttctccccg ccgaagcgac gctgccggaa atcatgtcct aaacttatcc
     3901 agagttttct cgcggcgccg gcgcgcggcg ggctaaatcc taattcctaa atcctccttt
     3961 ttttgataga gccgtccaaa tacctgtccc ttcgttggcg gattaaggac ggagagccga
     4021 cagcggacag acaagaatgc cgtcatcacg acgataagca aaactgccca cggcggtcag
     4081 gagcatttga aaatccggtt ttcgaacctt tttggaatca atcaggcgcg cgagctttgt
     4141 cagcgtttcc gtccctgcct gaagcggctt gtcgcctccg agtttgattt caatcagacc
     4201 gtaagcacca ttgcgtaaat gcacgacggc atcgcattcc agaccatgac ggtcgcgata
     4261 atggcgcacc gtcccgccaa gggcgtcggc atagaccctc aagtcgcgaa cggccatcgt
     4321 ttcaaacaag aggccgaacg tcgtgaggtc gttcatcagg tcttccgggc cgatttccaa
     4381 ggcggccgca gcaatcgacg gatcgacaaa ataacgtgca tcggaagtcc ggatggaatc
     4441 tttcgcacga agattgggat tccaggcagg catgtcctct acaacgaaca gacgcctcaa
     4501 ggcattgagg taagcgtaga tggtgccgtt gtcaatggac gtcatttcat tcgtggacag
     4561 atattctttg atgacacttg cggatgcttg cgtaccttgg agtcgggcat acgatcgcat
     4621 cagacgacgt gcccgatctt cgtcgcgcgt agtgccgtca acgcgcgaaa tgtccttctt
     4681 cgttatcgca tcgacatagt tgtaggcctg atcaagggcg gcatttccgg acagagagac
     4741 ggcttgcggc cacccgccgc ggcagacgat tcgggcgatt tcttccaact caagcggctt
     4801 tgctgaagcg gtctggacat tcgcgccgga gaacaattcc cggaaggaaa tctcgccgga
     4861 agattcctcg gactcccata gcgacatggg gcgcattttg agccaggcaa accgacccgt
     4921 acccgaatgt ttgatttcgt cttcgttggc cgggacggaa gaacccgtga ggatgaaacg
     4981 accgaggtct cccgcgtgat ccacatcgtg ccggacagca tcccagagga caggggcgat
     5041 ttgccattcg tcgatcagtc gcggacgggc acccgacagg atgcgtcccg gatccatccg
     5101 cgccatggcg atgttgtcgt ctcgcttctg gggttccgcc atgtagatga cgctctgggc
     5161 aacttgctca gcggttgttg tctttccaca ccacttaggc ccctcgatca ggacggcccc
     5221 cgtcccttct agtttacgcc gaagaagcga atcgactacg cgatccttgt atgctctcat
     5281 gagaaaaatc ttagcagaac gatccatcga aaacaaggcc gttccttcat ttgtcctaaa
     5341 ttcattcctt catttgtccc aactcgattc gtccatttgt cccaaaattg caggaacggc
     5401 ccgccgggga cgacccgccg gggacgaccc gccgaggacg gcgggctcca gggtacggat
     5461 tcccgccggg gacggcccgc accgtgaaca ggggatgaac aatgcggata caagaggaaa
     5521 aacagggtaa tcggcatatc gggttggttc cgggtttcgc accatgaaac aaatcccccg
     5581 cgccatgcca aaaaagccat tccccttgat gcgtgaagct cggagtgata tcctgtttcc
     5641 atcttttatc catccgcttg ggggtataac gctcaagggc gtaatcatgt caaacctttc
     5701 tcctacaccc ggttcctccc gtatcgtcaa gcatctgcgg acccgtctgg tttcaggtct
     5761 gctggtgttg atcccgttgg ccatcaccct gttcgtactg aacatcttcc tcaactccct
     5821 caccgccttt gcccggcccc tgctcaaacc cctgctgggg gtgttgcccg aatccgttct
     5881 gacgctgatc gcgttgaccc tcaccgtgtt gctgatctat ctgaccggtc tggtcaccac
     5941 ccatatcgtg ggccgccgcc tgatcctttt cggcgagcgg ctgctgttgc ggctcccggt
     6001 cgtcaaaacc atctacgccg catccaaaca ggtggtggaa accttctcct cgcaccagac
     6061 cacctcgttc caagcggttg tcatcgtgtc gttcccccgc cgcgaatcgc tcgcgatcgg
     6121 gttcatcacc ggcaccctgt ttgcgccgga caaccggaaa ctctatcggg tcttcattcc
     6181 caccacaccc aatccgacct cggggttcct gcttctgatg tcaccggaag atgttcaatt
     6241 cacggacatc cccatcgaag agggcattaa aatgatcgtc tccggcggca tgctcgcccc
     6301 ggaaaagtat gcggtggtgc cggataattg tccatgaaca aaaccgcacc gtctgccaag
     6361 ctggcggtgt aatctcgtcg aatcacggag gcaaaatggc taaaccccgt cgcgaagtgg
     6421 agatcacgct acacagcatc cgcgttccgg tcaccggcac ggggctcgat accgacacct
     6481 cccacctgct gaccgccacc ctgatctggc cgcgtctcgg cgtggcgggc aaaagcgcca
     6541 gccaaccctg cgcactgcaa cacggcgaat catctttcga aaccgtacat tggggacggc
     6601 gcattctctt taaagaggcg gtggagggcc gtttcgcttt gggactggtc ctgaccgagg
     6661 cgctggatga cgaagagatg gagaagttcc tgcgcttctg gaccggcgcc ttcctcgcgg
     6721 tcggcgccga cctcgcgggt gccgcggcac ccgcccccgc aggaaaactc gccgccgcgc
     6781 cactggatta cgcggccaag gcgatcgcca aatatcccgg cgcactgccg ctcgtcgagg
     6841 ggctggtcga gatcaacgcc gccgaactgc cgcccggcgg ccgggagcgg ttgctgaccg
     6901 tacgcctgac cgcggcccgc accttggcgc gcctttcccg ccgcacggtc aacagccgcc
     6961 cgcgcgtgag ccgctcgctc ttgctggaga agggggccga caatggcgag gtcaccctcg
     7021 ccatccgttc cctctgattc acttggctgt cacaaacgcc ggacgccgtc cgcggtacca
     7081 cgccagcatg accggggtgg cgatgaagat cgtcgaatac gtgcccgcga tcagaccaat
     7141 catcatggcc agcgcgaaat cgttgatcga tccgccgcca aatatgaaca gcgtcaccgt
     7201 tgcgatcaac gtggataacg aagtcaggat cgtgcggctc agcgtctggt tgatggcgcg
     7261 gttgcacaac tccgggaaag gcattttcac gtccttacgc atatcctcgc ggatacggtc
     7321 gaagatcacg atggtgtcgt tgatcgaata tcccacgatc gtgagcagcg cggccacaac
     7381 cgtcaggctc acctgccgcc cgaacaggct gtaaagcccc agcgtgatga aggcgtcatg
     7441 cgccaacgcc accacaccgc cgagcgcgaa gccgaactcg aaccgcaggg agacatagat
     7501 caagataccg atcagcgaga agagaatcgc cttggcggcg gataacttca ggtcaccgcc
     7561 gatctcggaa cccacatcct cctcgcccgc aagggtgaaa cggctctcgg gcagttcctt
     7621 cgccaacgcc ttctggatca ctttggaggc gctctcctca ccgatctggg tcacgctggt
     7681 tttcacgagc aacaggtttc ccgaaccgtc gagggtggac tggtactgga tcgtggcgtc
     7741 gtttttcacc gcatccgtga ccgccttgcg aacggtgccg atatccgcct tctgctcgta
     7801 ggtaaaggtc atcgagacgc ctcccgtgaa atcaaccgcc atgacgctgg caggatcttt
     7861 caatcctcgg gcaaagaaga tcgcgagcgt gaccacgatg atggccagcg acgtgtatcc
     7921 ggtcggcttg ccccaacgca aaaagtccac attcgagttc ttcatgaact gcagcatctt
     7981 gtagggccgg gtgcggtttt ccggcacggt ggcgttgaat atcaggcgcg tcaccaccaa
     8041 agccgtgaac atactgatga tgataccggc ggtcagcgtg atggcgaagc cccggatcgg
     8101 ccccgcgccg aagatgaaca ggatcacggc ggtcaacagc gtggtaatgt tggagtcgaa
     8161 gatcgccaga aacgcccggt catagcctgc cgccaccgcg gcgcgcgccg atttgcccag
     8221 cgcaaactct tcacgcatcc gctcaaagat cagcacattg gcgtcgaccg ccataccgag
     8281 gctcaaaacg atacccgcga taccgggcat ggtcagcacc ggcagatcca acacccgctt
     8341 ggcgatcgcc gtgtctttga cgaacacacc cagaatgctg gaggccatga tcaatccggc
     8401 cggcagcagc agcaggtcaa gcagcagcgc cacattcgcg atcaatccgc aatacgcgta
     8461 atagaccagc atgaagagcg cgaccagcac ggtggcgagg atcgatgctt ggatcccgct
     8521 ccggatcgcg tccgcgccca gcgtggactc gaccgagcgc ttctccagaa tcttcatcgg
     8581 cgcgggcaga gaaccggcat tgaggatgtt gcgcagcacg gcagcctcgg accaggagaa
     8641 attgccctcg atcacagcgc ggccgttggg gatttcggtc ttcagcaccg gcgcggaata
     8701 cagggtatca tccagcacga tcgccagacg gcgtcccacg tcgctgtctt tgttgcgcga
     8761 accgcgcggc gcgtacgcct tggtcagccg ggcaaaatca gcccctccct tggcattgaa
     8821 caccagcgag acatgcacgc ggccggtcat cggctcgatc tccaccgaag cgctggaaag
     8881 cgcgtctccg gtcatttcgg ccttgcgcag cacatagatc ggccggtaaa tcacctgccc
     8941 attcgccgtc tgctcgcgtt cgagcatgaa agcgtaacgg gggtccggca cctcgaacat
     9001 tgagagacgg tgcatatact cggggtcctt caccagctcg gtatagttcg gggcgcgctt
     9061 atagccgcgg ccgtcgtcag atgaaacgta cccttccggc aaacggcccg aggcgagcac
     9121 cttgtccaca agctgctcgt tctgcttgtg caccagcttg aattcaagaa aagccgcgct
     9181 ctggatactc tttt
//
LOCUS       DAOSLE010000110         9053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7271_length_9053_cov_7.601578, whole genome shotgun sequence.
ACCESSION   DAOSLE010000110 DAOSLE010000000
VERSION     DAOSLE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9053
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7271_length_9053_cov_7.601578"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PK576_07940"
     CDS             <1..278
                     /locus_tag="PK576_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61560.1"
                     /translation="RGFFGRLFRSRTAEAGASSRPATTRPTETEPYVPGSPIRVPTIQ
                     ADVKAVPEKQPYVASEKKEADRPFTAQAPDRGKNPLLRPRQGIREPE"
     gene            275..1393
                     /gene="rlmN"
                     /locus_tag="PK576_07945"
     CDS             275..1393
                     /gene="rlmN"
                     /locus_tag="PK576_07945"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOE61561.1"
                     /translation="MSAPDRPAAWGLLPSEWKPLCRERGLPGFRADQIVTGLYQTFAQ
                     SWDEFTTLPTELREQLSRSFPIEPLEVVHVHHAADGVRKLLLRCRDGECVETVLIPSK
                     GRLTQCVSSQVGCGFRCAFCASGQSGRTRDLTAAEIVGQVMAACQLLRADPQAPADPP
                     PAASAGGRPGAKPSLPRPGNIVVMGMGEPFDNYDNVLRALRILNDQKGINIGARHITI
                     STCGVVPGIRRLAEEGIQFELSVSLHAPDDALRNRLMPVNRRWPVAEVIAACRDYFAK
                     TGRVVTFEYTLVKGLNDTPQHADALSRLLRGMPCKINLIPLSPVEGFDGERPGDRACL
                     AFLDRLLRAGFATTLRQSRGRDVDAACGQLRLRKQAAK"
     gene            1587..1937
                     /locus_tag="PK576_07950"
     CDS             1587..1937
                     /locus_tag="PK576_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE61562.1"
                     /translation="MITRNGEREVERRGNMRGGTGTVQIEHWFRPEAFGAKVRLCARL
                     EIPPGGSIGTHRHEAEDEIYLVLSGQGLILENGRRIPVAAGDAVLTGKGGEHGVENPG
                     DEPLILAAIILLYA"
     gene            1956..4139
                     /locus_tag="PK576_07955"
     CDS             1956..4139
                     /locus_tag="PK576_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOE61563.1"
                     /translation="MTVPTQNHPGQAAEESVTGTVESIVYRNDENGYTVCHVRPHSRL
                     HSRDELVTVVGNCAAIWEGEELHAEGVWVRHKSHGPQFQARVITCITPTSVEGLRRYL
                     ASGMIKGIGPTFAKRIVDRFGEQTLDIIDRESGRLREVEGIGEGRRKLIKESWTEQRG
                     VREIMIFLQSHGIGTAKASRIYRQYGADSIAVVKRNPYRLCEDVWGIGFKSADAIALS
                     VGIPHDSELRACAGLIHTLRTEAEDGGHCFTVDADLLLHAQTMLDISVEILARALEQE
                     YGKGSLVKEENRIYLRDLYRAETRVAAKLRLLLDTPRTFKPIDAGKALAWAEGKMKIR
                     LAPLQREALCNALNTKVSIITGGPGVGKTTIIRALTEIFGARKLDVRLAAPTGRAAKR
                     MSEATGREAQTLHRLMKFNPNQNRFEFNDENPMTGDCFILDETSMIDIRLMDHFLQAL
                     PHSVTLILVGDTDQLPSVGPGNVLHDLIASGMIPFCRLDTIFRQDTSGLIVRNAHRVN
                     RGEPFETRVGESDFYFVETGEPDRIIARTLELMTQRIPRKFGFDPLTEVQVLTPMRRN
                     LLGTENLNAVIQAALNPNGPTLTRGSSGFRRGDRVMQIRNNYDKEVFNGDIGFVREVD
                     TEDRSLSVLFDGRPVAYAQSELDELVLAYACSIHKSQGSEYPAVIVLMHTQHYKLLQR
                     NLLYTAITRGKRLVLVVGSSRAVAIAIKANQVRERRTTLTARLKT"
     gene            complement(4165..5280)
                     /locus_tag="PK576_07960"
     CDS             complement(4165..5280)
                     /locus_tag="PK576_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOE61564.1"
                     /translation="MRKRGILCLAVIMGLAAWVGAQPLGPERMTEVKASVRKGLGWLA
                     AQQKPGGFWSDDKMPALTALPLWAWAGSGESAFREEADRAVAYLLSKQQPDGGIYTPT
                     PGRRGGGLGNYNTSICAAALYATGRREVMPAVLKARTYIASSQHIGDDWHAGGFGYDK
                     DAGRRYSDLNNTHFSLDAMRRTEGAEEFRPAGEKRADINWGAALRYALQLQNTEGETA
                     GGFAYNTQDPKGGVSTNAAGRVMLRAYGSMSYAGLLSLIHAQLDKQDPRVRSVVAYGS
                     RFWTLEENPGQGQQGLYFYYNIISRALSAAGLDALPKHAAPGEIHWREDLVRKLIALQ
                     RPDGSWVNENNRWWENDPVLATSYSLLALEFASGLTR"
     gene            5517..6773
                     /locus_tag="PK576_07965"
     CDS             5517..6773
                     /locus_tag="PK576_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE61565.1"
                     /translation="MKRGWLGAGAVWAALMGIGYAEPNRAPVPLWDLAVSPFAATTGR
                     IERVEGRVRMDGASAFAIPNRAIPDPSCFTIEMAIRFNALEEPSALCLLDQRVSDTGF
                     ALEALKWGRVGSPLTTTVNGIAYARNSFRASSNQVWRLVFAVRNGAVAVYQNNAPGPQ
                     YLLRVTPNLAPVWVGRTPLRKDRTRIADYEILSLKVYGPDLKYYLPGESADTMKNGYV
                     GGQGWLVRLPVKTDTPLPRILYYGDSISVGYGDRFTALLKDKAACYHWCHFVGGVRSF
                     PTAAFEAAASVAPFDIIVFNNGLHSLQWTPDKVTDEQIREVVRNMIRAFRKGAPKAKL
                     VWAATTPHTAKGRPVTELGELNPIVLRINTLADQVVKEEKIERVDLYTLLKDRLDLAA
                     GDQYHWSGEAYQLIAEALRKKCEELL"
     gene            6863..7795
                     /locus_tag="PK576_07970"
     CDS             6863..7795
                     /locus_tag="PK576_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOE61566.1"
                     /translation="MRLKTTCFGCRVALVFALGTLGRVSPLWAQEARRFATDPGNGAV
                     SMRLGDRTCWTYVPQSDEGKPYFHPLTIPGTDDVLTNFRPADHVWHLGFWFSWKGING
                     KNFWEPDPKGGTTRVVAQTVTPAPDLTFRAEATLDYVADGRTLVREQRAVRVETAPNG
                     DYTIEWDSTFFAREEGAVFSCTPAKRDQKGVWAAGGYAGLMWRFADSPAFTYTYTHDA
                     GRTNVMTCGESAARMEIVATAAASGARARITFRDHPENPRYPTPWFARHSAAAYQGRG
                     YYVVGPSMLFHEPLKLAPNTSARFRYTVAVERLR"
     gene            7962..8915
                     /locus_tag="PK576_07975"
     CDS             7962..8915
                     /locus_tag="PK576_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HOE61567.1"
                     /translation="MKKSCLIGVLAMTMMAAGAGEKGVCDARTVVEGEAPSLLPTGKR
                     FKLVWHDEFNGNQLDTSKWGYRTNFWGQRAHWFATPEDHAVEVKEGKCFLMLKKLPNG
                     QFVSPQLQTGELLWDYPVNPDAKSFWPLLEKRAPPKFEHKYGYYECRCRLQQEPGWWS
                     AFWMQTITQGCCLDPARAGIEHDIMESFVPGEVIPACFHYNGYGKEYKGFKTPRAVKN
                     APKGSANIQLDKTQYHVFGMLWEPDGYTIYVNGQQRGEKVGSKGDEAVSQVPEFVLIT
                     TEAKWYRNNRMTGKGVPELEEAAKKGDAFIVDYVRVFDLVD"
BASE COUNT         1733 a         2858 c         2829 g         1633 t
ORIGIN      
        1 cgcgcggctt tttcgggcgg ctcttccgaa gccgcactgc ggaggccggt gcatcgtcac
       61 ggcccgcgac gacgcgtccc accgagacgg agccgtatgt tccgggctcc cccatccgcg
      121 taccgacaat ccaggccgac gtgaaggcgg ttcccgagaa gcaaccttac gtggcgtccg
      181 agaagaagga agcggatcgc cccttcacgg cacaggcgcc ggatcgcggc aagaatccgc
      241 tcctgcgccc gcggcagggg atccgggaac ccgaatgagc gctccggaca gacccgccgc
      301 atgggggttg ctcccctccg aatggaaacc gctttgccgg gaacgcggtc tgcccggttt
      361 ccgtgcggac cagatcgtca ccggacttta ccagaccttc gcccagtcgt gggatgagtt
      421 caccacgctg ccgaccgaat tgcgtgaaca actcagccgg tcgttcccta tcgagccttt
      481 ggaagtcgtg cacgtccacc acgcggcgga cggtgtacgc aagctcctct tgcgttgccg
      541 ggatggcgag tgcgtggaga ccgtgctcat cccctccaaa ggccgtctca cgcagtgcgt
      601 ctcctcgcag gtgggatgcg gcttccggtg cgccttctgc gccagcggcc agtcgggccg
      661 cacacgcgac ttgaccgccg ccgagatcgt gggtcaggtc atggcggcct gccaactcct
      721 gcgcgccgat ccgcaggcac ccgccgatcc cccgcccgcc gcgtccgcag gcggcaggcc
      781 cggcgccaag ccctccctgc cgcggcccgg caacatcgtg gtcatgggca tgggcgaacc
      841 ctttgataac tacgacaacg tgctccgcgc cctgcgcatt ctcaacgacc agaaggggat
      901 caacatcggc gcgcgccata tcacaatcag tacttgcggt gtcgtgcccg gtatccggcg
      961 tttggctgaa gaagggatcc agttcgaact ctcggtttcg ctgcatgcgc cggatgacgc
     1021 gctgcgcaac cggttgatgc cggtcaaccg acgctggccc gtggcggagg tgattgcggc
     1081 ctgccgcgat tatttcgcga agaccgggcg cgtcgtcact tttgagtaca cgctggtcaa
     1141 gggcctcaac gatacgccgc agcatgccga cgccctgtcc cgcctgctgc gcggcatgcc
     1201 ctgcaaaatc aatctcatcc cgctctcccc ggtcgagggg tttgacggcg aacgtccggg
     1261 ggaccgcgcc tgtctcgcgt ttctggaccg gttgctccgt gccgggttcg cgacaacgct
     1321 gcgccagtct cgcggacgag acgtggatgc cgcctgcggt cagctccgtt tacgcaaaca
     1381 ggcggcgaaa tgatccgcgc gcgataccga cacgtggatg ggttccccgt gaagaaccgc
     1441 acaccctttt gcgcaaagga tccgttgacg gcggatgggg ccgttgcccg ttcacgcggt
     1501 tcatggtata gtccgggtcg cggcatgcgg gggcgcggat cccgtgaggc cggggtatgc
     1561 ggacacgcgc ggaaaaggga gtaaagatga ttacacgcaa tggggagaga gaggtggaac
     1621 ggcgcgggaa catgcgcggc ggcaccggca ccgtgcagat cgagcactgg ttccgaccag
     1681 aagcgttcgg ggccaaggta cgtctctgcg cacgtctgga aatcccgccg ggcggctcga
     1741 tcggcacaca ccggcacgag gcagaggatg agatttacct cgtgctctcc ggacaagggc
     1801 tgatcttgga aaatggccgt cgtatcccgg ttgccgcagg cgacgccgtc ctcaccggca
     1861 agggtggcga acacggtgtg gagaaccccg gcgacgagcc gctgatcctc gccgccataa
     1921 tcctgctgta cgcctgaccc cgtggtgggt ttcccatgac tgtcccgaca cagaaccatc
     1981 cagggcaagc agccgaagag agcgtgaccg gtaccgtgga gtctatcgtc taccgcaatg
     2041 acgagaacgg gtacacggtc tgccatgtcc ggccgcattc gcgcctgcac agccgcgacg
     2101 aactcgtgac ggtggtcggg aactgcgccg cgatttggga gggtgaggag ctgcacgccg
     2161 aaggcgtgtg ggtccgccac aaatcccatg gcccacagtt tcaggcgcgc gtcatcacct
     2221 gtatcacgcc cacctcggtc gaagggttgc gccgctatct ggcgagcggc atgatcaaag
     2281 ggatcggccc caccttcgcc aaacgcatcg tggatcggtt cggcgagcag acgctggata
     2341 tcatcgaccg tgaatccggc cgcctgcgcg aggtggaggg catcggcgaa ggccggcgca
     2401 agctcattaa ggagtcgtgg accgaacagc gcggtgtgcg cgaaatcatg atcttcctgc
     2461 aatcccacgg catcggcacg gccaaggcct cccgcatcta ccggcagtac ggcgctgatt
     2521 ccatcgccgt cgtcaagcgt aacccgtacc ggctttgcga ggatgtctgg ggcatcgggt
     2581 tcaaaagcgc ggacgccatc gcactcagcg tgggcatccc gcacgactcg gagctgcgtg
     2641 cctgcgcggg cctgatccac actctgcgca cagaggccga agatggcggg cactgtttca
     2701 cggtggacgc tgacctgctg ctgcacgccc agacgatgct ggacatctcg gtggaaatcc
     2761 tggccagagc cctggagcag gagtacggga agggctcact ggtaaaagag gagaaccgca
     2821 tctatctgcg cgacctctac cgggccgaaa cacgggtggc cgccaaactg cgcctgcttc
     2881 tcgatacgcc gcggaccttt aaaccgatcg acgccggcaa ggcgctggcg tgggcggaag
     2941 gcaaaatgaa gatccgtctc gcgccgctcc agcgcgaggc tttgtgcaat gccctaaaca
     3001 cgaaggtctc gatcatcacc ggcggtccgg gtgttggcaa gacgacgatc atccgcgccc
     3061 tcaccgaaat cttcggggcg cgcaaacttg atgtgcgcct ggccgcgccg accggccgtg
     3121 ccgccaaacg catgtccgaa gccaccggcc gcgaggccca gaccctccac cgtttgatga
     3181 agttcaaccc gaaccagaac cggttcgagt tcaacgacga gaaccccatg acgggcgact
     3241 gctttattct ggacgagacc tcgatgatcg acatccgtct gatggatcac tttttgcagg
     3301 ccctgcccca tagcgtcacg ctgattctgg tgggcgatac cgaccagttg ccgagcgtcg
     3361 gccccggcaa cgtcctgcac gatctgatcg cctccggcat gatccccttc tgtcggctgg
     3421 acacgatctt ccgtcaggac acctccgggc tgatcgtgcg taacgcgcac cgtgtcaacc
     3481 gcggcgaacc gtttgagacc cgcgtcggcg agagcgactt ctattttgtc gaaaccggcg
     3541 aaccggatcg gatcatcgcg cgcacactcg aactcatgac gcaacggatc ccccgcaagt
     3601 tcggattcga tcccctgacc gaagtgcagg tcctgacccc catgcgcagg aatctgctcg
     3661 gcaccgaaaa cctgaacgcg gtgatccagg cggcgctcaa tcccaacggg ccgaccctga
     3721 cgcgcgggtc ttcgggcttc cggcgcggcg accgtgtgat gcagatccgc aacaattatg
     3781 acaaagaggt gttcaacggc gacatcggct ttgtccgcga ggtggacacg gaggatcgtt
     3841 cgctgagcgt gctgttcgac gggcggcctg tggcctatgc gcaaagtgaa ctcgacgagt
     3901 tggtcctcgc ctacgcctgc tcgatccaca agtcgcaggg cagcgagtac ccggcggtga
     3961 tcgttctgat gcacacgcag cactacaagc tgctgcaacg caacctgctc tacaccgcca
     4021 tcacgcgcgg caaaaggctg gtgctggtgg tcggttcctc ccgcgccgtc gccatcgcga
     4081 tcaaggcgaa ccaagtccgc gaacgccgga ccaccctgac cgcgcgcctc aagacatgag
     4141 gccgcaacag ccaccgccgc cgcgtcaccg cgtcaggccc gaggcgaact ccagggccag
     4201 cagtgagtac gaggtggcca gcaccgggtc attctcccac cagcggttgt tctcgttcac
     4261 ccaagagccg tcggggcgct gcagggcgat cagcttgcgg acaagatcct cgcgccagtg
     4321 gatttcaccg ggcgcagcgt gtttcggcaa tgcgtcgagc cccgcggcac tgagcgcgcg
     4381 ggagatgatg ttgtagtaga aatacaaccc ctgctgtcct tggccggggt tctcctccag
     4441 agtccaaaag cgggaaccgt acgccacgac cgagcgcaca cgcggatcct gtttgtcgag
     4501 ttgcgcgtgg atcagcgaga gcagccccgc gtacgacatg ctgccatacg cgcgcagcat
     4561 cacgcggcca gcggcgttgg tggaaacccc gcccttcgga tcctgcgtat tgtaggcgaa
     4621 tccgcctgcg gtctcgcctt cggtgttctg caactgcagg gcatatcgga gggccgcgcc
     4681 ccagttgatg tccgcgcgtt tctcgcctgc ggggcggaac tcctcggccc cctcggtacg
     4741 ccgcatggca tcaagggaga agtgggtgtt gttcagatcg gaataccgcc gtcccgcatc
     4801 tttgtcgtat ccgaatccgc ccgcatgcca gtcgtcaccg atgtgctgcg aagacgcgat
     4861 gtaggtgcgg gccttcaaga ccgcaggcat cacttcgcgc cgcccggtgg catacagcgc
     4921 cgccgcacag atggaagtgt tgtaattgcc aaggcccccg cccctccgcc ccggcgtggg
     4981 ggtatagatg ccgccgtccg gctgctgttt ggacaacaga taggccaccg cgcggtcggc
     5041 ctcttcccgg aacgcgctct cgccagagcc ggcccaggcc cagagcggaa gcgcggtcag
     5101 ggccggcatc ttgtcatccg accagaatcc gccgggcttc tgctgtgccg cgagccaacc
     5161 gagcccttta cggaccgacg ccttcacctc tgtcatccgc tccgggccga gcggttgcgc
     5221 tccaacccat gccgccaacc ccatgatcac cgccagacac aagattcccc tttttcgcat
     5281 gatccgttcc tttcgcaccg ccctccccca aaagtgccgg gccgggccga ttccgtacag
     5341 ttgttaatat atcaaacgct gtcacggcct gtcaggttca taacgtaaac ggcatcctgc
     5401 ggtgtcggac gttttctccc cttgtgtgct gccggaggct gtagtatcgt tgaccccgat
     5461 cttttatcca cctccggtgc acgcgaacgc gtgtccggca acatgaggcc tttgcaatga
     5521 aaagaggatg gctcggggcg ggcgcggtat gggccgcgct gatggggatt ggctatgcgg
     5581 aacccaaccg tgcgccggtt ccgctgtggg atctggctgt ctcgccgttc gccgcgacaa
     5641 ccggacggat cgagcgcgtg gaggggcggg tgcggatgga tggcgcgagc gcgttcgcca
     5701 ttcccaaccg cgccattccg gatccctcct gtttcaccat cgagatggcg atccggttca
     5761 acgcgctgga ggaaccgagc gccctgtgcc ttctggatca gcgcgtttcg gacaccggtt
     5821 tcgctctgga agcgttgaaa tgggggcgcg tgggcagccc gctaaccacc acggtcaacg
     5881 gtatcgccta tgcgcgcaac agcttccgcg cttccagcaa tcaggtgtgg cggctggttt
     5941 tcgcggtgcg caacggcgcg gtggctgtct atcagaacaa cgcgcccgga ccacaatatc
     6001 tgctgcgcgt cacgccgaac ctcgcgcccg tgtgggtggg ccgtacgccg ctccggaagg
     6061 accggacgcg tatcgcggac tatgaaatcc tctcgttgaa ggtatacggt ccggacctga
     6121 agtattatct gcccggcgag tcggcggata cgatgaagaa cggctatgtc ggcggtcagg
     6181 gctggctcgt ccgcctgccg gtcaagaccg acacgccgct gccgcgtatc ctctactatg
     6241 gggattcgat ctccgtaggg tatggcgacc gcttcaccgc gctcctgaaa gataaggccg
     6301 catgctatca ttggtgccat ttcgtcggcg gggtccgttc cttcccgact gccgcgttcg
     6361 aggccgcggc gtcggtcgcg ccgttcgaca tcattgtgtt caacaacggg ctgcactcgt
     6421 tgcagtggac gccggacaag gtaaccgacg aacagatccg ggaagtggtg cgcaacatga
     6481 tccgggcgtt ccgcaaagga gcgccgaaag ccaagctggt ttgggccgcg accacaccgc
     6541 acaccgccaa aggacgtccg gtcactgagc tgggcgagtt gaaccccatt gtcctccgga
     6601 tcaacacgct ggcggatcag gtcgtgaagg aagagaagat cgagcgggtc gatctctata
     6661 ccctgctgaa ggaccggctg gacctcgccg caggcgacca gtaccactgg tccggcgaag
     6721 cctaccagtt gatcgccgag gcactgcgga aaaaatgtga agaactcttg tgagggaaaa
     6781 agcggacggt tccgtcaaaa caggtgatgc gttgagaacg gttgacggtg cgtaatggcg
     6841 ataacgccgg aaggagggag gcttgcgctt gaaaacaact tgcttcgggt gccgtgtcgc
     6901 gttggtcttc gcgctgggga ctctcggaag ggtgtccccc ctatgggcgc aagaagcgcg
     6961 gcggttcgcc accgatcccg gtaatggcgc ggtctcgatg cgcctcggcg accggacctg
     7021 ctggacttac gtgccgcaaa gcgacgaggg gaagccgtat tttcatccgc tcacgattcc
     7081 gggcaccgat gatgtgctca cgaacttccg tcccgcggac cacgtctggc atctcggttt
     7141 ctggttctcc tggaagggga tcaacggcaa gaacttctgg gaacctgacc ccaaaggagg
     7201 gaccacgcgc gtggtcgcgc aaaccgtcac gcccgcaccg gacctgacct tccgcgccga
     7261 ggccacactc gactacgtgg cggacggccg gaccctggtg cgtgaacaac gcgccgtacg
     7321 ggtggaaacc gcgccaaatg gcgactacac gatcgaatgg gacagcacct tcttcgcgcg
     7381 ggaggagggc gcggttttct cctgcacgcc cgcaaagcgc gatcagaagg gcgtgtgggc
     7441 cgctggcggg tacgcgggac tgatgtggcg cttcgccgac agtccggctt tcacctacac
     7501 ctacacccat gacgcgggac ggacaaacgt catgacctgc ggcgagtcgg ccgcgcgcat
     7561 ggaaattgtc gcgaccgcag ccgcgtccgg tgcccgcgcc cgcatcacct tccgcgatca
     7621 tcccgagaat ccgcgttatc ccacgccctg gttcgcgcgg cacagtgccg cggcgtatca
     7681 aggccgcggt tactatgtcg tggggccctc catgcttttc catgagccgc tcaagcttgc
     7741 cccgaacaca tccgcgcgtt tccgctacac cgttgcggtt gaacgcctcc gataaggggg
     7801 acgcatcagg caaggacggg cagacgctcg ttgaaacatc ggtattgact cttttcagcc
     7861 ccccgagata atagggcccc tattcagggt tccgaaccgt ttccgggaca aacccaaagg
     7921 gatacgcgtg atccccgaca caaaaaaggg aggatggaag cgtgaaaaaa tcgtgcttga
     7981 tcggtgtgct ggccatgacc atgatggcgg ccggggcagg ggaaaaaggc gtgtgcgacg
     8041 cccgaacggt cgtggagggc gaggctccgt cgctcttgcc gacagggaag cgctttaagc
     8101 tcgtttggca tgatgagttc aacgggaatc aactcgacac ctcgaagtgg ggttaccgca
     8161 ccaacttctg gggacagcgc gcgcattggt tcgcgacacc ggaagaccac gccgtggagg
     8221 tgaaggaggg caaatgcttc ctgatgttga aaaagcttcc taacggtcag ttcgtctcgc
     8281 cgcaactcca aaccggcgag ctgctctggg attatcctgt caatcccgac gcgaaaagtt
     8341 tctggccctt gttagaaaaa cgcgcgccac cgaaattcga gcacaaatac gggtattacg
     8401 agtgccgttg ccggttgcag caggaacccg gatggtggag cgccttctgg atgcagacca
     8461 tcactcaggg ctgctgtctt gatcccgcgc gcgccggcat tgaacacgac atcatggaat
     8521 cgttcgttcc cggcgaggtg attccggcgt gcttccatta caatgggtac ggcaaagaat
     8581 acaaagggtt caagactcca cgcgcggtca agaacgctcc gaaggggtca gccaacatac
     8641 agctcgataa gacccagtac catgtcttcg gcatgctctg ggaaccggat ggctacacga
     8701 tatacgtcaa tggccaacag cggggagaga aggtgggctc gaaaggggat gaggcggttt
     8761 cgcaggttcc cgagttcgtg ctgatcacca ctgaggcgaa atggtaccgc aacaaccgca
     8821 tgaccgggaa aggggttccc gaacttgagg aggccgcgaa aaaaggcgat gccttcatcg
     8881 tcgattacgt gcgggttttc gaccttgttg actgacgttg aatttcgaac cctgaacatc
     8941 gaactttgag cgctgagcct aaaaagagca gttgaacctt caactctctt tggtttctgg
     9001 gtttctggtt tcaaggtttc agggtttcaa ggtttcatcc aaatcggtat cgg
//
LOCUS       DAOSLE010000111         8941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7380_length_8941_cov_7.661152, whole genome shotgun sequence.
ACCESSION   DAOSLE010000111 DAOSLE010000000
VERSION     DAOSLE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7380_length_8941_cov_7.661152"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1081
                     /gene="nagB"
                     /locus_tag="PK576_07980"
     CDS             314..1081
                     /gene="nagB"
                     /locus_tag="PK576_07980"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOE61568.1"
                     /translation="MDITICPSAEEASRLAGEVINNAVRQKPDLVLGLATGSTPLLLY
                     KEMIAACSNGLDYSRVRTFNLDEYYGVPPDHPQSYRKFMNDNLFSHLNIAPENTHVPD
                     GKARDVTAHCAAYEAEIKAAGGVDIQVLGIGSDGHIGFNEPASSLASRTRRVTLTRQT
                     ISDNARFFASADEVPRYAISMGVGTILEARTCLLLCFGANKARAVRGAIEGGISQFNP
                     ASALQLHPDTRVYLDEEAAAELDLKEYYRWAAENRPQ"
     gene            complement(1083..1541)
                     /locus_tag="PK576_07985"
     CDS             complement(1083..1541)
                     /locus_tag="PK576_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE61569.1"
                     /translation="MDEHAKIEVIARGVCVAGGQILLCHGRGSSLTYLPGGHVEFRET
                     ARQALEREIREELGCASEAGAFLGCCEHAFMQNGAPHAEVNLVFALRVPGLAPGAEVR
                     AAEAWIGFRWHALDDLAASGLEPAPLRTRIADWLRNPGGHVVSGEAWQDV"
     gene            complement(1583..2971)
                     /locus_tag="PK576_07990"
     CDS             complement(1583..2971)
                     /locus_tag="PK576_07990"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOE61570.1"
                     /translation="MGYTNGMISVLEELASRRRFGMRPGLETIQALLEDLGHPERETA
                     AIHIAGTNGKGAVAAMCEAVLRAAGYPVARYTSPHLVCLNERFLLNGKPVEDAALEAC
                     AREVGTAVRALESRTGTEVTFFECLTAIAFVLCRNAEIRLIALETGLGGRLDATNTVT
                     PLVSVITRIGRDHCDWLGDTLEQIAAEKAGIIKPGRPVVCAAMPEEARAVIAQSASRV
                     GSRFADAAETVEVTVTRNGLEGQTLKIATPNRSLPPIRLPLAGAFQVENVCAAVTALE
                     EVDACGVSLSDAAFVKGLEEVCWPGRFQLAVQTPPVIVDGAHNPEGARAIRQALKACR
                     IRPPLGLVAGFCGDKDALGHLRILAPVVKRAWAVPVPNPRTLTPEQTAGVMRMAGLGD
                     PQACAKLGAALDAAREWAAAEGGTVLVCGSLFLAGAALVDLGAYPWASPRHDPNDVLR
                     SPTPAEREQTRG"
     gene            3046..4545
                     /gene="tilS"
                     /locus_tag="PK576_07995"
     CDS             3046..4545
                     /gene="tilS"
                     /locus_tag="PK576_07995"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE61571.1"
                     /translation="MSATVQHTPAPAVPEGLGQAVAEEVTRKRLLRGIRCLGLAVSGG
                     ADSVALFHLLRPLCEAAGIRTVVFHLNHGLRGEAADGDACFVRSLAHAAGIACITGRA
                     DLAGRTPDGQSPERVARDARLAFFRQAAEETGAEAIATGHQADDVAETLLLRLARGAG
                     ATGLAGLRPRTSLPRSTRILIRPLLSFPGAVLRAWLRDRGLDWREDHSNLDRTIPRNR
                     IRHALIPDLERFLQPGLRTRLCRSAAILREEDALLETLAGRALDALAPLQPEGLPDSL
                     PAVALLEHPLALQRRILRLWLFRQACPEAAGFAGVDALLDMCRDGKGRSVCLPGGKTF
                     RLHDGLLQLQPAREIPPPETSLSCPGVCRWGALEIVAEFSRGITGTVTGINRYPAVCT
                     LCADALAGRALLVRSRRPGDRIAPVGLNGTKKIQDLFTDEKIPEAVRDAIPLFECDGE
                     IAWVPGYRIGRRFAVPAADAASVRVTVRRAETDAVRGAPPRPDESDQPR"
     gene            complement(4533..5924)
                     /locus_tag="PK576_08000"
     CDS             complement(4533..5924)
                     /locus_tag="PK576_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE61572.1"
                     /translation="MSGQLTRRAMLSRLGLGGTAASMSLLAGRGFADADTDGDPSYPG
                     FAKNIRKVAPSMQDVPSARIEGGKVIQPARELPVFHTTDVVVVGGGSAGFAAAVAAAR
                     TGAKVALVERYGSLGGLFTNGMVLIVIGTGVKEGGAFKLVTRGICEEFLRRLEKMGPG
                     AITKLTDTVGQPTADPEAAKVLMDEMIQEAGVEMFFHAWGVDVIQNGNAVQGVVFESK
                     QGRQAILAKVVVDASGDGDVYFQAGAGYQQISHAMGFVYRVGNADRVDKTKLPKGTKP
                     PRLGSAEPIPSARWMNNLGPKGNGLDIRELSKIEMMHRKSAWNYVQKIRGTPGHEEVF
                     LMQTTPQIGVRATRLLDGVTSIDKKSAAAQTKFPDVVAVSGHDGLRLPEFQIPYGALL
                     PKTVDNVIAAGRCIACGADLIDRVRLIPVCIVTGHAAGVAAALAAQAGVRPRDVPAAD
                     IQKVLRGQGAYLG"
     gene            complement(6007..7527)
                     /locus_tag="PK576_08005"
     CDS             complement(6007..7527)
                     /locus_tag="PK576_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61573.1"
                     /translation="MNRAACLAAAWFLVLSCSGAGPEESASDTGADPVREAVSLFINA
                     RTAGGLDAFIVAVERVRKLAESGQPFFRFLLAVYAAHEPLCKLTDTARQRYLEEGRPL
                     MSRHAETGNALAQYLVGVDCELNLAQPEDARRWYERAAMQGQALAQNRLGLMYYQGQG
                     VAPDDGRAAAYFRQAALQADMNGEYNLATLYAAGRGVPKDEELAFHLFRRAAEKGHAH
                     AMNNLGWLYQKGVGVAADPARAAEWYRKAAESGNADGQFNYGFLCAQGVGVPQDDREA
                     GLWYWRAAEQGHVEAMVSIGMLYRDGRGLKGDDFKALNWFAKAAEHRHAGAQFFIGQM
                     LEQGRGLPRDTERAFRWYQKSAQQGYPRAHHALGTLYYRGRGVEKDYVKAAGHFALAA
                     EKGLASSQNNLGMLYLRGEGVERDEKQAAAWFAKAVRQGVAGAAFNLAQLYEYGRGVD
                     ASHTKAVQLCKEAALRGHVGAADWLLQNGYTAWAKPHREKKNRDAQVSEGLEDQPF"
     gene            complement(7524..8351)
                     /locus_tag="PK576_08010"
     CDS             complement(7524..8351)
                     /locus_tag="PK576_08010"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE61574.1"
                     /translation="MRDNPDTRLDRTLCSLPLRRELSSVLHFLHTQARGARVGLDIGF
                     THAGVSQILRQTGGYWMSVETSPERRNLVASVLGSDTVLCAGADGTLPFEDKQFDVVV
                     MAHGTLLSGAAAADAIRECHRVLKTGGHFILTVEYRKRVSIAAAINRQRVVTGAGVRY
                     NAEDIFHLLKDGFDVLGFRHTCRFWVQLVRQWVDRRQASGMCGGMQNRIRFLYGLARI
                     LDIPLFLSRGYQMTVYGRRKGWRGKHSRVISGITPVSDALLSDPRRERRGMSLMRYK"
BASE COUNT         1596 a         3176 c         2571 g         1598 t
ORIGIN      
        1 ctgcggtgtc gggaacgcgg gcgtccctgt cgatcgtcgc gggtcgaatg tcgcgggtcg
       61 cgggtcgacg atcccgatac cgatagcgat accgatagcg atcccgatac cgatagcgat
      121 cccgaccccg atgaaacctg aaacccagaa acccagaaac cagaaaccca gaaacccaga
      181 aaccagaaac ccagaaacca aggagcattg aaaggttcaa ctgctctttt taggagttgc
      241 cgtatttgac acccgcgccg atttgaatta gtcttacatg tgattcatat cattacaaac
      301 gaagggagcg attatggaca ttaccatttg cccttccgcc gaagaggcca gccgcctcgc
      361 aggggaagtt atcaacaacg ccgtccgtca gaagcctgat ctcgttttag gcctcgccac
      421 cgggagcacg ccgctgttgc tctacaagga aatgattgcg gcctgcagca acggtctgga
      481 ttacagccgc gtgcgcactt tcaacctcga tgagtattac ggggtaccgc cggatcatcc
      541 gcaatcctat cggaaattca tgaacgacaa ccttttctcg cacctgaaca tcgcgccgga
      601 gaacacccac gtgccggatg gcaaggcccg cgacgtgacg gcccattgcg ccgcatatga
      661 ggcggagatc aaggctgcgg gcggtgtgga cattcaggtg ctggggattg gcagcgacgg
      721 ccacatcggc ttcaacgaac ccgcctcctc tctcgcctca cgcacgcgac gggtcaccct
      781 cacccggcag acgatcagcg acaacgcgcg ctttttcgct tccgccgacg aggtgccgcg
      841 ctacgccatc tccatgggcg tcggcacgat cctcgaagcg cggacgtgcc tgttgctctg
      901 tttcggcgcg aacaaggccc gggcggttcg gggcgcgatc gaaggcggga tctcgcaatt
      961 caaccccgct tcggccctgc aactccaccc cgacacgcgg gtgtatctgg acgaggaggc
     1021 cgcggcggaa ctcgatctga aagagtatta ccgctgggcc gccgaaaatc ggccgcagtg
     1081 agtcagacgt cctgccaggc ctcgccggag accacatgac cgcccggatt gcggagccag
     1141 tccgcaatcc gggtccggag cggtgcgggt tctaatccgg aggcggccag atcatcgagc
     1201 gcatgccacc gaaagccgat ccacgcttcc gctgcgcgga cttccgcgcc gggcgcaagg
     1261 cctggcacac gcaacgcgaa cacgagattg acctccgcgt gcggtgcgcc gttctgcatg
     1321 aaggcgtgtt cgcagcaacc gaggaacgcc ccggcctctg acgcgcaacc cagctcttcg
     1381 cggatctcac gctccaacgc ctgacgcgcc gtctcccgga attcgacgtg tccgcccggc
     1441 agataggtga gcgaggatcc ccggccgtga cagagcagga tctgtccccc ggccacacac
     1501 acgccccgtg cgatcacctc gatcttcgca tgctcgtcca taccttctcc tatggtttca
     1561 atctgcgtaa tctgcagaca ggctatcccc gcgtttgttc ccgttccgcg ggcgtcgggc
     1621 ttcgtaacac gtcattcgga tcgtgacgcg ggctggccca cgggtaggcc ccaaggtcca
     1681 ccaaagccgc gcccgcgaga aacagcgagc cgcacaccag cactgtgccg ccttccgctg
     1741 cggcccactc ccgcgccgca tccagcgccg caccgagttt cgcgcacgct tgggggtcgc
     1801 ccaacccagc catgcgcatc acccccgcag tctgctccgg cgtcagcgta cgcggattcg
     1861 gcaccggcac agcccaggcc cgcttgacca cgggcgccag aatccgcaga tgccccaagg
     1921 cgtctttgtc gccgcagaaa cccgccacca accccagcgg cggtcgtatc cggcacgctt
     1981 tgagcgcctg ccggatcgcc cgcgcaccct cgggattgtg cgcgccgtcc acgatcaccg
     2041 gcggtgtctg caccgccaac tggaaacggc cgggccagca gacctcttcg agtcccttga
     2101 caaacgccgc atcggacaga ctcacaccgc aggcatccac ctcttccaac gccgttacag
     2161 ccgcgcagac attctcgact tgaaacgcgc cggccagcgg cagacggatc ggcggcagcg
     2221 accggttcgg cgtggcgatt ttgagcgtct gcccctccag cccgttccgc gtcaccgtca
     2281 cctccaccgt ttccgcggca tccgcgaaac gcgagccgac ccgtgacgcg ctctgtgcga
     2341 tcaccgcgcg cgcctcttcc ggcatagccg cgcacacgac cgggcggccc ggcttgatga
     2401 tccccgcctt ctccgcggcg atttgctcca gcgtgtcccc gagccaatcg cagtgatcgc
     2461 gtccgatccg ggtgatcacc gacaccagcg gcgtaacggt gttcgtcgcg tccagccgcc
     2521 cgccgaggcc ggtctccagc gcgatcagcc gtatctccgc attccggcac aggacaaacg
     2581 cgatggcggt gaggcactcg aaaaaggtga cctctgttcc ggtccgggac tcgagcgcgc
     2641 gcaccgcagt gccgacctcc cgcgcacacg cctccagcgc ggcatcctcc accggtttcc
     2701 cgttgaggag aaaacgctcg ttcaggcaca ccagatgcgg cgaggtgtat cgcgccaccg
     2761 gatacccggc cgcccgcagc acggcttcgc acatcgccgc caccgcaccc ttgccgttgg
     2821 tgcccgcgat gtgtatcgca gccgtctcgc gttccggatg tcccaaatcc tccagcagtg
     2881 cctgaatcgt ctccagcccc ggccgcatac cgaaacgccg tcgggacgcc aactcttcca
     2941 acacagagat catgccgtta gtgtagccca tcccgacgcg ccccacaatt cccgattgcc
     3001 ggaatccggt tgtgccgcac tgcggttttc tcgtatgatg ggcacatgtc cgctaccgta
     3061 caacacactc ccgcgcccgc cgtccccgaa gggctcgggc aagccgtggc agaggaggtg
     3121 acgcgcaaaa gactcctgcg cggcatccgg tgcctcggtc tggcagtgtc gggcggcgcg
     3181 gactcggtcg cgctcttcca tctgctgcgt ccgctttgcg aagccgcagg gatccgtacc
     3241 gttgtgttcc acctgaacca cggcctgcgc ggcgaagccg ccgatgggga tgcgtgtttc
     3301 gtgcgctcgc tggcacacgc ggcgggtatc gcgtgcatca ccggccgcgc cgaccttgcg
     3361 ggccgcaccc cggacggcca atccccggaa agggtggcac gcgacgcgcg cctagccttc
     3421 ttccgacagg ctgcggagga aaccggggcg gaggccatcg ccacgggaca tcaggcggac
     3481 gatgtggccg agaccctcct gctgcgtctc gcgcgcggcg cgggcgccac cggtctcgcg
     3541 ggactccggc cgcgcaccag tctgccgcgc agcactcgga tcctgatccg cccgctcctt
     3601 tcctttcccg gcgcagtgct gcgcgcttgg ttgcgcgacc gcggtctcga ctggcgcgag
     3661 gatcattcta acctggatcg cacgatcccg cgcaaccgga tccgccacgc cctaatcccg
     3721 gatctggagc ggttcttgca gcccggtctg cgcacgcgac tttgccgttc cgccgcgatc
     3781 ctgcgcgaag aggacgccct gctcgaaacc ttggctggcc gcgcgctcga cgcgctcgca
     3841 ccgctccaac cggaaggcct cccggactcc ctgccggccg tcgcgttgct ggagcacccc
     3901 ttggcattgc agcggcggat tctgcgcttg tggctcttcc ggcaggcgtg tcctgaagct
     3961 gcggggttcg cgggtgtgga cgccttgctc gacatgtgcc gcgatgggaa aggccgatct
     4021 gtttgcctcc ccggcggaaa aacattccgc ttgcacgacg ggttgctcca actccaaccc
     4081 gcgcgcgaaa tccctccgcc ggagacctcc ctgtcctgtc cgggagtttg ccgctggggc
     4141 gcgttggaga tcgtggccga attttcgcgc ggcatcaccg gaaccgtcac cggcatcaac
     4201 cgttatccgg ccgtgtgcac gctgtgcgcg gacgcgctcg cgggacgcgc gttgctcgtg
     4261 cgcagccgaa gacccggaga ccgcatcgcg ccggtcggtc tgaacggcac aaaaaaaatc
     4321 caggacctct tcacggatga aaagatcccg gaagcggtgc gcgacgccat tccgcttttc
     4381 gaatgcgacg gggagattgc ctgggtgccc ggctaccgca ttggacggcg tttcgccgtg
     4441 cccgcagccg acgccgcgag tgtgcgcgtc acggtgcgtc gggcggaaac ggacgctgtg
     4501 cgcggcgcgc ccccccggcc cgacgagtcg gatcagccca gataagcgcc ctgcccgcgc
     4561 aggaccttct gaatgtcggc agccggcaca tcgcgcggac ggactccggc ttgggcggcc
     4621 agtgcggcgg ccacacccgc cgcatgcccg gtcacgatgc agaccggaat cagacgcacc
     4681 cggtcaatca ggtccgctcc gcaggcgatg cagcgtccgg ccgcgatcac gttgtccacc
     4741 gtcttgggca gcagcgcccc gtacgggatc tggaactccg gcagacgcag accgtcgtgt
     4801 cccgagaccg ccaccacatc ggggaacttc gtctgggcgg ccgcgctttt cttgtcgata
     4861 ctcgtcaccc cgtccaacaa ccgtgtcgcg cgcacaccga tctgcggcgt ggtctgcatc
     4921 agaaagacct cttcatggcc cggcgtgccc cgaatcttct gcacgtaatt ccaagcggat
     4981 ttgcggtgca tcatctcgat cttcgacaac tcgcggatat ctaaaccatt gcctttgggt
     5041 ccgaggttat tcatccaccg cgccgacggg atcggctcgg cactgccgag acgcggcggt
     5101 ttcgtgccct tcggcagctt ggtcttgtcg acacggtccg cattccccac gcggtagacg
     5161 aatcccatgg catgggagat ctgctgatat cccgcgcccg cctgaaaata gacgtcgccg
     5221 tcacccgagg catccaccac caccttggcg aggatcgcct gccgtccctg cttgctctcg
     5281 aacaccacgc cctgcaccgc attgccgttc tggatgacat ccacgcccca ggcgtggaag
     5341 aacatctcga cccccgcctc ctggatcatc tcatccatca gcactttggc ggcctcggga
     5401 tctgccgtgg gctggcctac cgtgtcggtc agcttggtga tcgcacccgg gcccatcttc
     5461 tccaagcggc gcagaaactc ttcgcagatg ccgcgtgtga cgagcttgaa cgcgccaccc
     5521 tccttcacgc cggttccgat cacgatcagc accatgccgt tggtgaacaa cccgcccaga
     5581 ctgccgtacc gttcgaccag cgccaccttg gcaccggtgc gggccgctgc caccgccgcc
     5641 gcgaacccgg ccgaaccgcc gccgaccacc accacgtccg ttgtgtggaa aaccggtagc
     5701 tcgcgcgccg gttggatcac cttgccgccc tcgatccggg cggaaggcac gtcctgcatc
     5761 gacggcgcga ctttacggat gtttttcgcg aaccccgggt aagagggatc gccgtccgtg
     5821 tccgcatccg caaaaccacg gccagccaga agcgacatgc ttgccgccgt cccccccaat
     5881 cccaacctgc tcaacatcgc tctacgcgtc agctgtccgc tcatcgttat ccctcccgga
     5941 tggtttatcc tcgtttcacc ataacgcttt tcttccgccg ctgtcaatcc gccgcgcgcc
     6001 cttgtttcag aacggctgat cctccagccc ttccgacacc tgcgcgtccc ggttcttctt
     6061 ctcccggtgc ggtttcgccc aggcggtgta gccgttttgc aacagccaat cggcagcgcc
     6121 cacatgtccg cgcaacgcgg cttctttgca gagttgaacc gctttggtgt ggctcgcgtc
     6181 cacgccgcgg ccatactcgt agagttgcgc cagattgaaa gccgccccgg cgactccctg
     6241 ccggacggcc ttggcgaacc atgccgccgc ctgtttttca tcgcgctcca caccctcgcc
     6301 gcgcagatag agcatcccca gattgttttg ggaagaggcg agaccttttt ctgcggccag
     6361 ggcaaaatgt ccggccgcct tcacataatc cttctccacc ccccggccgc ggtagtacag
     6421 cgtccccagc gcatgatggg cgcgcgggta accctgttgc gcggacttct gataccagcg
     6481 gaaagcgcgc tcggtgtcac ggggcagacc gcgcccctgt tccagcatct gcccgataaa
     6541 gaactgcgcc cccgcatgcc ggtgctccgc cgctttggcg aaccagttca gcgccttgaa
     6601 gtcatcgcct ttcaatcccc ggccatcgcg gtaaagcatg ccgatgctca ccatggcctc
     6661 gacatgcccc tgctccgccg cgcgccaata ccagagcccc gcctcacggt catcctgcgg
     6721 caccccgaca ccctgcgcgc acaggaaacc gtaattgaac tgcccgtccg cattcccgct
     6781 ttccgccgcc ttgcggtacc actccgcagc ccgcgccggg tctgcggcaa cgcccacccc
     6841 cttctgatac agccacccca gattgttcat ggcatgggca tggccctttt ccgctgcccg
     6901 ccggaacaga tgaaacgcca gttcctcgtc tttcggaact ccgcgccctg ccgcatacag
     6961 cgtggcgaga ttgtactcgc cgttcatgtc agcctgcagc gcggcctgac gaaaatacgc
     7021 ggcggcccgg ccgtcatccg gcgcaacccc ctgcccttga tagtacatca ggcccagccg
     7081 attctgggcc agagcctgac cctgcatagc ggcgcgctca taccagcggc gcgcgtcctc
     7141 cggctgcgcc aaattgagtt cgcagtccac accgaccaga tactgggcca gcgcgttgcc
     7201 ggtctcggca tgccgcgaca tcagggggcg tccctcctcc agatagcgtt gccgcgccgt
     7261 gtcggttagc ttgcagagcg gctcgtgcgc cgcatagacc gccagcagga aacgaaaaaa
     7321 cggctggccc gattccgcca gcttccgcac ccgctcgacc gccacgatga acgcatccag
     7381 cccccccgcg gtccgtgcgt tgataaacag ggagaccgct tcccggaccg gatcggcccc
     7441 ggtgtcggac gctgattctt ccggcccggc gccgctgcac gacagcacca gaaaccacgc
     7501 cgccgccaaa cacgccgccc gattcacttg tacctcatca gcgacatgcc gcgcctttcg
     7561 cgtcggggat cggagagcag cgcgtccgaa accggcgtga ttcccgaaat cacccgggag
     7621 tgttttccac gccacccttt ccgccgcccg tacacggtca tctgataccc tcgcgacaga
     7681 aagagcggga tatcgagaat ccgggccaac ccatatagga aacggattcg gttttgcatc
     7741 ccgccgcaca tcccggaggc ttggcggcgg tccacccatt gccgcaccag ctggacccag
     7801 aaccggcagg tgtggcggaa ccccagaacg tcgaagccgt cttttagcag atgaaaaata
     7861 tcctccgcgt tgtaccggac accggccccc gtcaccaccc gctgccggtt gatcgccgca
     7921 gcgatgctga cccgcttgcg gtactcgacc gtcagaatga aatgcccccc ggtcttgagc
     7981 acccggtggc actcgcgtat agcatcggca gcggccgcgc cggacagcag ggttccgtgc
     8041 gccatcacca ccacatcaaa ctgcttgtcc tcgaacggca acgtcccgtc cgcgcccgcg
     8101 cacaacacgg tatcgctgcc cagaaccgat gccaccaagt tccggcgctc gggcgaggtt
     8161 tccacactca tccagtaacc gcccgtctga cgcagaatct ggctcacacc ggcatgggtg
     8221 aaaccgatgt ccaagcccac ccgggcgccc cgcgcctgcg tgtgcaggaa atgaaggaca
     8281 gaactgagtt cacgccgcaa cgggagggaa cacagagtcc gatccaagcg tgtgtctgga
     8341 ttatcgcgca tcacaactgc tttccaaggt gaatccagct taacacgctg aagcggggtt
     8401 cgtcaacaaa cccgcctgaa aaaccgacgt gcaggcacat caccgattcc cttgaaaccc
     8461 cgccggcagg gatcgttccc cagtacggag cgaatacatt cgtatattca gtaatcggtt
     8521 cgtgacaggg ggggggagtc atcgggtacg ggtacggggt atggccaacg ttcacgttct
     8581 tcaaatcgcc atcgggatcg gtatctctat cggtatcgct atcggtatcg gaatcgattg
     8641 tttcgccacg gaacactttt ttggtttcta gtttcagggt ttcagggttt ctggtttcag
     8701 ggtttcaggg tttcaaggtt tcatccaaat cggggtcggg gtcggggtcg ctatcgggat
     8761 cgattattta accacgaaca cacgggatgg cactgctcgg gttttttgga acgccagggg
     8821 gaattcctga ttttcatgaa gttttatgtg tttcaaggtt tcatccaaat cggggtcggg
     8881 atcggtatcg ctatcggaat cgtcgacccg cgacccgcga cattcgaccc gcgacgatcg
     8941 a
//
LOCUS       DAOSLE010000112         8936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7385_length_8936_cov_10.889314, whole genome shotgun sequence.
ACCESSION   DAOSLE010000112 DAOSLE010000000
VERSION     DAOSLE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7385_length_8936_cov_10.889314"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..911)
                     /locus_tag="PK576_08015"
     CDS             complement(126..911)
                     /locus_tag="PK576_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOE61575.1"
                     /translation="MDAFSRTALLLGQPAIKRLHASRVAVFGLGGVGSYVVEALARAG
                     VGRFLLVDHDRISVSNLNRQILALHSTLGRLKTEVAAERVRDINPRAEVETRALFYLP
                     ETADAIDLAACDYVVDAVDTVSAKVTLAVCAAEAGVPIVSCMGAGNKLDPMRFEVADL
                     FATSVCPLCKVMRREMKARGIRALRVVYSREPPLLPASGEDAAYAECRRPTGSVPSVP
                     AAAGLLLAAEVIRSLTDAGDLERMKRKGRACAPGESPQHTEGI"
     gene            complement(932..1786)
                     /locus_tag="PK576_08020"
     CDS             complement(932..1786)
                     /locus_tag="PK576_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61576.1"
                     /translation="MRWQERVAGRRILTAAVLCLAGAASGTAERYVPKPFEYYQPILD
                     RMPFGAAPAQTGAGAQDAESAKSAAQEKIEQERLAKQVNMSAVNITPEGRTAIGFTDL
                     SQKPPVNHYLLVGDEADGWKVLEADYDLETATIEKEGVAITLKLGQGLQPPAAAAAGA
                     PAAGARKAGEVPVASVLPPGLTRRVRRPVAGTVAGAGGGPASGTAPVTKPPPSSYVER
                     NRERALQREKEEQASREKMQAQLAELARKAALNEMRRRDQEEAEARAAEQAEEAAAAQ
                     QEEEQSAL"
     gene            complement(2085..3686)
                     /locus_tag="PK576_08025"
     CDS             complement(2085..3686)
                     /locus_tag="PK576_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE61577.1"
                     /translation="MQAESKRPDKTMRIEVTPSPTERQPRDTAVSATKGRGRTWRTQC
                     LKKRNTPFYKLLESVYDAVLITSHSGQILESNERAVEFFQIPCSDLIGQSVLRLISGA
                     TESLLDTIHNNLINKKYTLVEARCKRADGTSFAAEIAVNQVDLDATGQLCFFVRDITV
                     RQQAQQDLEHAMERIQAHDRARMEFVSNVSHELRTPLTSMIYAVSNMLRGVIGPMPEK
                     AVRYLERLQSDCHRLLATVNDILDLRQVENRTLVLTRKVAPLGAVIREGAETLQVQAD
                     AKRITMRFDFAERELFCWCDAQKIERVVLNIVGNAVKFTPEGGTITLTLTQDPDQSKL
                     ALLTVSDTGLGIPPEVLPKVSQRYFRVGDHVSGTGLGLSISREIVELHGGSLAFTSPV
                     PGTDCGTAVSVRLPLAPKPLVLVYSQDPDLCDTLRERIARLGYAVALAGSAAEALNEC
                     WERQPALFILDIRITDTDVRDLVLRLREEPKTKRLPVMVIGCQAWQRNEISLYQNLNL
                     FCVAWPLREQELPRNLALAVLGKLR"
     gene            complement(3765..4154)
                     /locus_tag="PK576_08030"
     CDS             complement(3765..4154)
                     /locus_tag="PK576_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61578.1"
                     /translation="MSQCVISNPVTIGIGAGVIGFVAGYLIAKIGGNGASRYASGGDG
                     DCVEIYVGNLSYDMTEAQMRKEFERYGTVKSARIITNRFNHKSKGFGFVEMPNRPEAE
                     AAIKALHDRDVLGRKLRVNEAKNKSHE"
     gene            complement(4295..6703)
                     /gene="rpoD"
                     /locus_tag="PK576_08035"
     CDS             complement(4295..6703)
                     /gene="rpoD"
                     /locus_tag="PK576_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOE61579.1"
                     /translation="MAAKKTKAGHGAASAKKKTGKSLKKAAGAKPVKGGKKPAEKTRK
                     PSSKRAAEEEKRVRPAVKPVSKAVVKSTSKPAAKSTAKPAKKPAPKPTAKSAAKSAAK
                     PVAKPAVKSTAKPAKKPASKPAAKSAVQPKKKSAPKPTSTAKMPASDPDISKAKNADS
                     DELKKPDADPGEAVEEGQDDLNFDTEELVHLSVASETGLAGGLQESDDLDDFGIDNGE
                     TYEAGLEIGEGEEVEGEFEGAEFTVGYETELALDANHHLEAKVKREERSERIKALIKR
                     AESQGGYLTYDNINEMLPSAVIKDTDIESYLAILRGMDIEIIDASEVERFRSEHEQEA
                     QSGRGTRMDFFDDPIRMYLHQMGQVPLLTREQEVEICKRIEVAESKEREMFNQFAFTP
                     RLYLELIDRLETGMERFDRVVTDKYADSRDAYIEGAGKLKKNLQNVRNRILKAYEAVS
                     ACARNAPKRKRLEKALEKARLELFNVFQSLNFKQKILESLCTEADEKIYRPYKALLIE
                     QAKLNKQRQTKKRDQTLQEIAVRMRSIEQEFGMDPEEFLERFEELREVLRSGQKARTE
                     MVEANLRLVISIVKKYMNRGLSFLDLIQEGNTGLMKAVEKFEYRRGYKFSTYATWWIR
                     QAATRAIADQARTIRIPVHMIETINKLLRVQKKLVQELGREPTPEEAAEEMDIPVERV
                     RAVYRMAQQPISLQSPVGDGDDAHFGDFIEDKSAENPSEMTAYSMLKERLQEVLTTLT
                     DRERQVLDFRFGLTDGYSRTLEEVGKQFNVTRERIRQIEAKALRKLRHPTRLRKLEGF
                     LETR"
     gene            complement(7149..8480)
                     /locus_tag="PK576_08040"
     CDS             complement(7149..8480)
                     /locus_tag="PK576_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61580.1"
                     /translation="MKRRDFLKSAMTTGAGLILLPGGLRGATAASNKLNIALIGTWGR
                     GLAHFDSIADENVVALCDCWERNLADAVKRFPKAKTYIDWRKCLEQKDIDAVVICTTD
                     FTHAFIANWALNRNMHVYLEKPLGITVEEARVVRANWLPKRNKLATQVGMQRHSHDNF
                     NRVREMIHDGAIGELKSAYAWGNRKIPKPGYLPAAGAPPAGLHYDLWLGPVKTDHPFN
                     PEYFAGGPGANCLKWNMYWDFGIGQIGDMGSHTMDLLWNAVDCGLPTTAEGKGEPFNP
                     DVTPVNAELRFEHPANDWRGPIGISWYQGGAMPNPPSSYVDIKKIGHGAMFKGSEGIL
                     VADFSSRALIPLGKDGSLSYYKPRKKEDLLPSVGHFQKQWIKACKNPSLKTACDFEYS
                     ADMIEQMCLGLVAYRVGKKLQYDGKAGRVTNSAEADALLKREYRAGWTLNG"
BASE COUNT         1536 a         2887 c         2548 g         1965 t
ORIGIN      
        1 ggatgacagg attttcagga ttgaacaaca tcatgttaat catgtcctaa acttattcaa
       61 atctaaacac ctgtcccttc gttattattt gagaagaaaa acgcctggag ccggatcatg
      121 tcgcatcaga taccttccgt gtgctgcggg gattcgccgg gagcgcatgc cctccccttt
      181 cttttcatcc gttccagatc ccccgcgtcg gtcagggaac gaatcacctc tgcggcgagc
      241 agcaatcccg ccgcggcagg cacagagggg acgctgccgg ttggacggcg acactccgca
      301 tacgcggcat cctccccgct agccggaaga agcggcggtt cgcgggagta caccacgcgt
      361 agcgcgcgta tcccgcgcgc cttcatctct ctgcgcatca ccttgcagag cgggcagacc
      421 gaggtcgcga acagatcagc cacctcaaag cgcatcgggt cgagtttgtt accggcaccc
      481 atgcaactca cgatcggcac gcctgcctct gccgcgcaca cggcgagcgt caccttggcg
      541 cttaccgtgt cgaccgcgtc caccacgtaa tcgcaggccg cgagatcaat ggcatcggcg
      601 gtttccggca aatagaacag cgcacgcgtc tcgacctccg cccgcgggtt gatatcccgc
      661 acacgctcgg ccgccacctc cgtcttcagc ctcccaagcg tactgtgcag ggcgagaatc
      721 tggcggttga gattggaaac gctgatccgg tcatgatcca ccagcaggaa gcgtccgacc
      781 ccggcgcggg ccagcgcctc gaccacatac gaacccaccc cgcccaaacc gaagaccgcc
      841 acgcggctgg cgtgaagccg ttttatggct ggttgcccca acagcagggc cgttcgtgag
      901 aaagcgtcca tgcgcggcgt cccatccgga atcacagggc cgactgctcc tcttcctgct
      961 gcgcggctgc ggcctcctcc gcctgttcgg cggcccgcgc ctccgcctcc tcctgatcgc
     1021 ggcgccgcat ctcattaagc gcggcctttc gcgccaactc ggcgagctgt gcctgcatct
     1081 tctctcgcga cgcctgctcc tccttctcac gttgcagggc acgctcgcgg ttccgctcga
     1141 catacgagga gggagggggc ttcgtcactg gcgcggtgcc gctggccggt ccgccaccag
     1201 cgccggccac tgttccagcc accggccgcc gcactcggcg agtgagcccc ggagggagaa
     1261 cgctcgctac cggaacttcc cccgctttcc tggctcccgc tgcgggtgcg cctgcagccg
     1321 cagcggcagg gggctgtagc ccctgtccga gtttcagcgt gatggcgaca ccctccttct
     1381 cgatggtcgc ggtctccaga tcataatcgg cttcgagcac cttccagccg tccgcctcgt
     1441 cgccgaccaa cagataatgg ttgaccggcg gcttctgcga gagatcggtg aatccgatcg
     1501 cggtgcgccc ttcgggtgtg atgttcaccg ccgacatgtt gacctgcttg gcgagtctct
     1561 cctgctcgat cttctcctgc gccgcgctct tcgcgctctc ggcatcctgc gctccggcac
     1621 ctgtttgcgc gggtgcagcc ccgaacggca tgcggtcgag aatcggctgg taatattcga
     1681 acggtttggg cacataccgt tcggccgtgc cggaagcggc acctgccaga cacaggacag
     1741 ccgccgtgag gatccgcctt cccgcgaccc gctcttgcca ccgcacatga ttccccgtac
     1801 cgttcatgtc agaaagtata gcagaccgga ccacagtcgt gaaagaggaa ttccaccgcg
     1861 tgtgccgctc tgggcgcatc aaggcaagcc ggaatgaaca agatttctgt tcgcgttttc
     1921 gtctgcgtgc gccactcctt gcctaaagct gctaaattgc caggattaca ggattttcag
     1981 gattgaacaa catcgtgtta atcatgtcct aaacttattc caaggttttt tcgcggcgga
     2041 gccgcgcggc gggctaactt ctgtctcccc tttccggccc ccggtcaccg gagtttgccg
     2101 agaacggcca acgccagatt tcggggcagt tcctgttccc gcaagggcca ggcaacgcaa
     2161 aacaggttga ggttctgata gagtgagatc tcgttgcgtt gccaggcctg acagccgatg
     2221 accatcacgg ggagccgttt ggttttgggc tcttcgcgta gccgcaacac cagatcgcgc
     2281 acatcggtat cggtaatgcg gatgtcgaga ataaaaagcg cgggctggcg ttcccaacac
     2341 tcgttcaggg cttcagcggc cgacccggcg agggccaccg cataccccag tcgggcgatg
     2401 cgttcacgca gcgtgtcgca cagatcggga tcctgcgaat agaccaacac cagcggttta
     2461 ggcgcgagcg gcaggcggac cgagaccgct gtgccacagt cggtgcctgg aaccggactg
     2521 gtgaaggcga ggctgccgcc gtgcagttcc acgatctcgc gggagatcga caggccgagg
     2581 ccggttccgc tgacatggtc cccgacacgg aaatagcgtt gcgacacctt gggcagcacc
     2641 tcgggcggga tacccaaccc tgtgtcgctc accgtcagca gggccagttt cgactggtcg
     2701 ggatcctgcg tcagcgtgag ggtgatcgtg ccgccctcgg gtgtgaattt gaccgcattg
     2761 cccacgatat tgagcaccac acgctcgatt ttctgcgcgt cgcaccagca gaacagctcg
     2821 cgttccgcga aatcgaagcg catcgtgatc cgcttggcgt ccgcctgcac ttgcagggtt
     2881 tcggcgccct cgcgaatgac cgcgccgagc ggtgccacct tccgggtgag aaccagcgta
     2941 cggttctcca cctgacgcag atcgaggatg tcgttgaccg tggccagcag acggtggcag
     3001 tcggactgga gccgctccag atagcgcacc gccttctccg gcatgggccc gatcaccccc
     3061 cgcaacatgt tactgaccgc atagatcatg gaggtcaggg gcgtgcgtaa ttcgtgagaa
     3121 acgttggaga cgaactccat ccgcgcccgg tcatgcgcct gaatgcgctc catcgcatgt
     3181 tcgaggtcct gctgcgcctg ctggcgcacg gtgatgtcgc gcacgaaaaa acagagttgt
     3241 cccgtggcgt ccagatccac ctggttgacg gcgatctcgg cagcgaagga ggtgccgtcc
     3301 gcccgtttgc aacgggcctc gaccagcgtg tatttcttgt tgatcaggtt gttgtggatg
     3361 gtgtcgagca gcgattccgt cgcaccggag attagccgca gcaccgactg cccgatcaga
     3421 tcgctgcacg gaatctggaa aaactccaca gcccgttcgt tgctttcgag aatctggccg
     3481 gagtgcgaag tgatgagcac cgcgtcgtag acactttcca gcagtttata aaacggtgtg
     3541 ttccgcttct tcaggcactg cgtgcgccag gtgcggccgc gccctttcgt cgcgctgacc
     3601 gcagtgtccc gcggttgcct ttcggtcggc gacggcgtga cttcgatgcg catggttttg
     3661 tccggacgct tcgattcggc ctgcatgaca cccctgttag cggattcgtg tttccgtgtg
     3721 cctagtgaaa aggcgccccg gaaccggggc gccttccgat cgggttactc gtgcgatttg
     3781 ttcttcgcct cattcacccg cagtttgcgg cccagcacat cacggtcgtg cagcgccttg
     3841 atcgccgcct ccgcctccgg ccggttgggc atttcgacaa agccgaagcc cttggacttg
     3901 tggttgaagc ggttggtgat gatgcgcgcg gatttgaccg tgccgtaccg ctcgaattcc
     3961 ttacgcatct gcgcctcagt catgtcgtaa ctcaagttgc cgacatagat ctcgacacag
     4021 tcgccgtctc cgcccgaagc gtaacggctg gcaccgtttc cgccgatttt ggcgatcaga
     4081 taaccggcta cgaaaccaat cacgcccgca ccgataccga tcgtcaccgg attggatatc
     4141 acacactggc tcaaacgaac ctccttgggt ttttctgttt gctcggacca cacgactctg
     4201 ccgggggcag cactggaacc ccgtgcacac aacctgtcgg tgccggggct tgcgggccgc
     4261 gccgcgcttg atcgcgcgac gcacagaccc cgcctcagcg cgtttccagg aatccctcca
     4321 acttacgcaa ccgtgtgggg tgacgcaact tgcgcaaggc tttggcctca atctggcgaa
     4381 tgcgctcgcg cgtcacgttg aactgttttc cgacctcctc aagcgtgcgg gaatatccgt
     4441 cggtcaggcc gaaccggaaa tccagcacct gacgctcacg gtcggtcagt gtcgtcagca
     4501 cctcctgcag gcgctccttc agcatgctgt aagcagtcat ctccgaagga ttctccgcag
     4561 acttgtcctc gatgaagtcg ccgaaatggg cgtcgtcccc gtcccccacc gggctttgca
     4621 gcgaaatggg ctgctgggcc atacggtaaa cggcgcgaac gcgctcgaca gggatgtcca
     4681 tctcttcggc agcctcttcc ggcgtgggct cacgccccag ttcctgcaca agtttcttct
     4741 gcacgcgcag aagcttgttg atcgtctcga tcatgtgaac cggaatgcgg atcgtgcgcg
     4801 cctgatccgc aatcgcccgg gtcgccgcct ggcggatcca ccaggtggca tacgtactga
     4861 acttgtatcc ccggcggtac tcgaacttct ccacagcctt catcaggccg gtgttcccct
     4921 cctgaatcag gtcgaggaac gagaggcccc ggttcatgta cttcttgacg atgctgatca
     4981 ccagccgcag attggcctcg accatttcgg ttctggcctt ctgcccggag cgcagcacct
     5041 cgcgcaactc ctcgaagcgt tcgagaaact cctcggggtc catgccgaac tcctgctcga
     5101 tcgaccgcat tcgtaccgcg atctcctgca gggtctggtc ccgtttcttg gtctgccgct
     5161 gcttgttcag cttggcctgc tcgatcagca gcgccttata cggccggtag atcttctcat
     5221 ccgcctcggt gcagagactc tccagaatct tctgcttgaa gttcagactc tggaacacgt
     5281 tgaacagttc gagccgcgcc ttctccaacg ccttttcgag acgcttgcgt ttgggtgcgt
     5341 tccgggcgca ggcagatacc gcctcgtacg ccttcaggat ccggttgcgg acattctgca
     5401 ggttcttttt gagcttgccc gccccttcta tgtaggcgtc gcggctgtcc gcatacttat
     5461 cggtcaccac ccggtcaaat cgttccatac cggtttccag ccggtcgatc agctcgagat
     5521 agaggcgcgg cgtgaaggcg aactggttga acatctcgcg ctctttgctc tcggccacct
     5581 cgatgcgctt gcagatctcc acctcctgct cgcgggtgag cagcggcacc tggcccatct
     5641 gatgcagata catgcggatc gggtcatcaa agaaatccat acgggtcccg cgcccgctct
     5701 gcgcctcctg ctcatgctcg ctgcggaacc gctccacctc cgacgcatca atgatctcaa
     5761 tatccatgcc gcggagaatc gccagatacg attcgatgtc cgtatccttg atcaccgcac
     5821 tcggcagcat ctcgttgatg ttgtcgtagg tgaggtatcc gccctgcgac tcagcccgct
     5881 tgatcagcgc tttgatccgc tcgctacgct cctcgcgctt aaccttggcc tccagatgat
     5941 ggttcgcatc cagcgccagt tccgtctcgt acccgaccgt gaactccgcg ccctcgaact
     6001 cgccctcgac ttcttcaccc tcgccgattt ccagtcccgc ctcgtaggtt tcgccgttgt
     6061 cgattccgaa atcgtccaag tcatcgcttt cctgcagccc gcctgccaac cctgtttcgg
     6121 acgcgacact gaggtgcacc aactcctcgg tatcgaaatt caggtcgtcc tgcccctctt
     6181 caaccgcctc gccgggatct gcgtccggtt ttttcaactc gtcggagtcc gcgttcttgg
     6241 ccttggaaat gtctggatcg gatgccggca tcttggccgt tgaagtcggc ttcggcgcag
     6301 acttcttctt cggttggacc gcggatttgg ccgcaggctt ggaagcgggt ttcttcgcgg
     6361 gtttcgctgt ggacttaacc gcaggtttcg ctacgggttt ggcagcagat ttcgcagcgg
     6421 acttggccgt aggcttggga gcgggtttct tcgcgggttt cgctgtggac ttggccgcag
     6481 gttttgacgt ggatttcacc acggccttgg aaaccggttt cactgcgggc ctgactcgct
     6541 tttcttcttc tgcggcgcgt ttggacgaag gcttcctggt cttctccgca ggcttcttgc
     6601 cccctttgac gggcttggcc ccggcagctt ttttcaagct cttccccgtc tttttcttgg
     6661 cggacgctgc gccatgaccc gctttcgttt tttttgctgc catcggaatc gcacccctta
     6721 tatcggaacc tgaacaaggc ctcgctgccg caaccgggca gacttcacct tatacgccga
     6781 aaatggaggt taaactttaa tgaaagccgg ggagcgtgtc aagcggtgcg cggcaacagc
     6841 agcgattccg ggcgcattct cgagtccctg accgttgagg tttcggggtt cggcgtcacg
     6901 cttgtcggcg cggcgccgga ccgacgaaac cgtgccccgc ctccgggcag ggatacctcc
     6961 ccgaggcgtc cgcctcaccc aacaaacaaa acgtcgcagg tcgaatgtcg cgggtcgcaa
     7021 gtcgacgcac acgagaacgg gggcttgatt tcatgaagtt tcatgtggtt catggtacaa
     7081 aacccggaac ccgaaacccc ttttccgccc gtctgccgtc tgttttttcg cgcttcccgc
     7141 agagggggtt acccgttcag ggtccagccc gcacggtatt cgcgcttcag cagcgcgtcc
     7201 gcctccgcac tgttcgtgac ccgtccggcc ttgccgtcat actggagttt cttgcccacg
     7261 cggtaggcca ccagaccgag gcacatctgc tcgatcatgt ccgcgctgta ctcgaagtcg
     7321 cacgcggtct tgagggaggg gtttttgcag gccttgatcc actgcttctg gaagtgcccg
     7381 accgatggca gcagatcctc cttcttcctc ggcttgtaat aggagaggct cccgtccttc
     7441 ccgagcggga tcagcgcgcg cgaggagaag tcagccacca agatgccctc gctccccttg
     7501 aacatcgcgc cgtggccgat ctttttgatg tccacgtacg agctgggagg attgggcatc
     7561 gcgccgccct gataccacga gataccgatc ggtccgcgcc agtcgttggc cggatgctcg
     7621 aagcgcaatt cggcgttgac cggcgtcacg tcgggattga acggctcacc cttgccctcg
     7681 gcggtggtcg gcagaccgca gtccaccgca ttccagagga ggtccatggt gtggctgccc
     7741 atgtcgccga tctggccgat gccgaaatcc cagtacatgt tccacttcag gcagttcgca
     7801 cccggaccgc ccgcaaaata ctcggggttg aacggatggt cggtcttcac cggacccagc
     7861 cacaggtcat agtgcaggcc tgcgggcggc gcgcccgcag ccgggagata tccgggtttg
     7921 ggaatcttgc ggttacccca ggcgtacgcg ctcttcaact cgccgatcgc gccgtcgtgg
     7981 atcatttcgc gcacgcggtt gaaattgtcg tgcgaatggc gctgcatgcc gacctgcgtc
     8041 gcgagtttgt tccgcttggg cagccagttg gcccgcacca cacgcgcctc ctcgaccgtg
     8101 atgcccaacg gtttctcgag atagacgtgc atgttgcggt tcagcgccca gttggcgatg
     8161 aacgcgtgcg tgaaatcggt cgtgcagatg accaccgcgt caatgtcttt ctgctccaga
     8221 catttgcgcc agtcgatata ggtcttcgcc ttggggaagc gtttgaccgc atccgccagg
     8281 ttacgctccc agcaatcgca caacgccacg acattctcat ccgcgatcga atcgaaatgc
     8341 gccaacccgc gtccccatgt gccgatcagc gcgatgttca gcttgttgct cgcggccgtg
     8401 gcaccgcgca acccgcccgg cagcaggatc aggcccgcac cggtcgtcat cgctgatttc
     8461 aggaaatcac gtcgtttcat cctcttcctc cctgttatgg aggctgcgcc tccttgatcc
     8521 gtaccgccga ctcccctttt ttgaatctgg cggctttctt ttcacgtttt agaatatcat
     8581 ggtcgcgaag ccgcgggcga gtccaatttt cgtgcacctc ttacgaatcc ggccacaaac
     8641 agcattgcgc gttgaacacc aagatgcccc tgcttttcat gaaggttccg ttttagggtt
     8701 tctgggtttc tggtttcagg gtttcagggt ttctggtttc aaggtttcaa ggtttcatcc
     8761 aaatcggggt cggggtcggg atcgctatcg ggatcgggat cgactgtttc accacgggac
     8821 actttcttgg gtttctggtt tcagggtttc agggtttcat ccaaatcggt atcgggatcg
     8881 ctatcgctat cgggatcgat tatttaacca cgaacacacg ggatggcact gctcgg
//
LOCUS       DAOSLE010000113         8860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7446_length_8860_cov_8.443157, whole genome shotgun sequence.
ACCESSION   DAOSLE010000113 DAOSLE010000000
VERSION     DAOSLE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7446_length_8860_cov_8.443157"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(425..5200)
                     /locus_tag="PK576_08045"
     CDS             complement(425..5200)
                     /locus_tag="PK576_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61581.1"
                     /translation="MRKRHCAMVLWCGLAGSVLANVSGTWIAPVGGLWSDTNNWQDGV
                     IPFGRGHTATFSAAAVSVTNDGARPVGVLKFNQTNIAIYGNAIVLDATGPKLIANNGT
                     AYLHVPVVATNPLTVTKETHTVSGVQLSRRNELEAGWVQVKSGVRLTSDARAMDPLPG
                     EDDAIRAPLSVRDQDLTLSAGGIFCLLGSAAAPTEQRFGVLNVKDYGGLDVVNGGFLA
                     PAELLVDDLAGPGLLSLSGGGRVAVASANRFVGTIRNPGADLRIEGTASPVLKPAADP
                     IMHLDASDLDSFTFAEENGTNFVVEWFSQVPGNYVRHDGHLTEGQRALPWVRTNVLNG
                     LPVVDFGNMIKTNKTERSLAPYLIWNTRSTAVKAAFAVLRTQNFIFTDTDKGHYHHSV
                     LSSGGMFRWDQTMLTSRGETAASFKNGEYVISLNGVPVTDPFSQKLSATEFDVVAMVV
                     KEGVTSVGTIAQDRSYRFGGQQVAEEIFYDRILTDAEIEATVAYLRQKWQGMEADAGM
                     AVAGPGALRVHVSDSNRSRLTVETGAAFAVETLSGSGIREVGGNGTLQVERGGSRPSR
                     PLALQGGTLALVNQGRAINTTLETAMDIPGLYFQLDASDLSSMTLDGDRVLEWRGNSN
                     VTNGLLAYAINTADNPAPRWVPGRLNGLPVVDFGAWGAGALLHWNHTNTAIQTLFMVF
                     DHIHPESFWLSDINDGNYANFHRGVNGLIFNTSYVTAGLQTGQVYLNGNRIDQMATFV
                     PEEEPFLISFVSTDGSAARAACMATDRYPRPGLLQYRSGGQRIAEVLVFNRHLNAAER
                     EQIEGYLMRKWLPNIPARFAASDGSQTFDGVVTSAADPVAVKVDTGQQAVLGALDGNG
                     PVEKTGAGTLVVGQVAGLTAPLSVKEGRLAFDTRVLPDPYVLPGGIAYHVDASDAASV
                     LLDADGVSVTNILDASGGSLSAQPSDPGIPPQLLPDALNGLPVISFRGAESGCAALWS
                     AKVSGIRTVFWVCGSQDGGGMPLGTASTAEGSDFKRTPILSAHSPIWAPESLAQHGVT
                     RIHGAVVNGRTTGFNGGYQLMSLVTDRGCSASAFATHKNVELFGGQRLAEVIIYQRLL
                     SDVERRDVEAYLARKWFGAPTSGYAGGRVDMNDLNMEGGTVDLPVGAAVSIRRFTGTA
                     DLLKNDDSVLSIADLSALSGSVVVSNGTFVLAGKPVQTELPVDGMLMHMDASVTGSFE
                     VVSENGTNFVTRWNSLVGSAYAAHDNISKRPWLLENELGGSPVVNFGPFKRQGWGDTA
                     ADGAYLIWDQPRLNIRSGFMVLGSQDGGNFLVGWNGSGGVADFHRGYGSGGYVDASGS
                     IIATGRTEPPLWIKSANAYWSVDGVRVNPGTTSLSGGYQAICFMSDPEHAPTNMLRGS
                     GFAIDRTFRWGGQRLAEFAIYDRLLTEEERLAVEVYLRNKWFGVLPTGYYQNGNVSNL
                     SVFAGGVVDVNGQARSVTHLGGNGTLSNGTLVVTGSLAPGAAEGQVGTLTADSLTLGD
                     GVVMRVDLAGGTCDRVFLNGMLTFGGAGTIVLSHTGTPTEGVVGDYMLFSASAIKTPE
                     FAEQWVVEGLPPGTGGKVCVSGTNVILSVFAQGTLLLMR"
     gene            complement(5330..5860)
                     /locus_tag="PK576_08050"
     CDS             complement(5330..5860)
                     /locus_tag="PK576_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE61582.1"
                     /translation="MRKWMWVLWMPVLTSLAQDAGCPAAVQQEVKSYVWVKGDAAKQE
                     PVLREAAAFLEKAGHFYLATCEGDSPRVRPIKYVFIVDNKLLFVTSAKKGMYQQLLKN
                     PKVEISRTAVDKSAYLRFCGRAVLCGDAAVKAKLLEMEPVFGKKFGEDQAIFLVEPER
                     VGLFPMKGGEVRSKSF"
     gene            6053..7246
                     /locus_tag="PK576_08055"
     CDS             6053..7246
                     /locus_tag="PK576_08055"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61583.1"
                     /translation="MAEKKKRVGMIGGGEASFMGPIHRAAIEQSGCVELVCGAFGSTR
                     QSSFETGRMLNLPPRRSYGTYRDLFRREASFPKTERIDFVTVLTPNAMHYPIAMSSID
                     ASFPIFSEKPFTCNLDEAVNLTRKLRNSGLAYGVAMVYPCYPALRKAQDLIQKDKAIG
                     VVRKVVATFSLGWMAQRLETAGNKQAGWRTDPRRSGPAGCLADLGSHAFYLSEWLTGL
                     SVSEVCADLRPTVAGRILDDDCTLLVRFETGARGVFLCSQIATGRQEGLAIEIYGDKA
                     ALGWSQSRPGRLTLTAIDGQREELTVNGSDEEEPLQTPPSPYGNNRAYIAALAECYRS
                     FAATLDRPKSQKTEPLPLYMSVEEGLRSVAFVDAVLQNTAVPEEDQPLPAKWMPVVVP
                     PVLDL"
     gene            7412..8713
                     /locus_tag="PK576_08060"
     CDS             7412..8713
                     /locus_tag="PK576_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HOE61584.1"
                     /translation="MTIQQGKTFTGFCLLSFCAAQGFAAGFGLYEASARGNAMGGALV
                     GATGDASANYFNAANLTELDGTHTMAGVTLVHPVLDITANGDKNNLDSGWFYPPHLYA
                     SQQLTEHWFTGFGVYTEYGLGTRYDRDWPLNWSSTKTTLESLTVNPNLAYKVNERLSL
                     GAGFRAMYLDFYHTKTPYQHDTIGVLPTPLPPLPVYGSMRTKVEGDSWGYGYNLGFSY
                     KITDTLDAGFVYRSRVRHTVKGDIEVQSSADTALPVPIPNPLPHGHASARGEIELPPS
                     WTAGLNYRPIDRLNLGFAATYTEWSSYDDLTITFGPTLANSDKLRRSSEEKNWNDVWR
                     LGIGADYMLTERWSVQCGYVYDMDPISKNNTDTMLPPGDRHIISTGLGYAVQTWTIHA
                     SYGLIVMKSCERTVTNGTKRAETDFDNGFCHLIALSVGKRF"
BASE COUNT         1843 a         2939 c         2397 g         1681 t
ORIGIN      
        1 gtttctgggt ttcatccaaa tcggggtcgg ggtcgctatc gggatcggga tcgatacagc
       61 ctcacagtct cacagcctta ccgcctcacc gcctttgaga ttacgggctc cgcccgcatc
      121 tcaatacggt gcggcgggga ccgcgacaaa agccaatgtt tacggggctt tgctagaatt
      181 ctacccgttc aactgctcct tttgggttca atcctgaaaa ttctggatac atgcgcccgc
      241 gcagcgggaa atcctgtcag ttccagcaca tatccttgag cccaggcctt catcccccct
      301 ccttcacgtt ccgtggcatc aggtttactt tacccaagcc ttcaggtgtc gggccggccc
      361 gtccggaccg gcccgaccga catctcctcc gctgtgcgca aagcggcgtt gctgcgggcg
      421 acgttcaccg catcaggaga agcgtgccct gcgcaaagac cgacaagatc acattggtgc
      481 cggatacgca taccttcccg ccggttcccg gcggcagccc ttcgacaacc cactgttccg
      541 cgaattcagg cgttttgatt gcagaggcgg agaaaagcat gtagtcgccg accacaccct
      601 cggtgggcgt gcccgtgtgc gacaggacaa ttgtgccggc tccgccaaag gtcagcatgc
      661 cgttcaggaa gacccggtcg catgtgccgc ccgcaaggtc cacacgcatc accacgccat
      721 cgcccaacgt cagactgtcc gccgtgaggg tccccacctg accctccgcc gctcccggcg
      781 ccagagaacc ggtcacaacc agcgttccgt tgctgagggt cccattgcct ccaaggtgcg
      841 tcaccgaacg cgcctgtcca ttaacatcca ccacgccacc ggcaaagacc gacaggttcg
      901 agacgtttcc gttctgataa taaccggtcg gcagcacacc gaaccacttg ttacgcaggt
      961 aaacctcgac cgccaaccgc tcctcctctg tcagcaagcg gtcatagatc gcgaattcgg
     1021 caagccgctg tccgccccag cggaaggtcc ggtcgatcgc gaagccactg ccgcgcaaca
     1081 tgttggtggg cgcgtgttcc ggatcactca tgaaacagat ggcctgatac cctccggaga
     1141 gcgaggtcgt gcccggattc acacgaaccc cgtcgacgct ccaataggcg ttggcggatt
     1201 tgatccacag cgggggctcc gtacgacccg tggcgataat cgaaccgctg gcgtccacat
     1261 acccaccgga tccgtacccg cggtggaaat ccgcgacgcc gcccgagccg ttccatccga
     1321 ccagaaagtt cccgccgtcc tgggatccga gaaccatgaa cccgctgcgg atgtttagcc
     1381 gcggctgatc ccaaatcaga tacgcgccgt ctgccgcggt gtccccccat ccctgacgtt
     1441 tgaaggggcc gaagttcacc accggcgaac cgcccagctc gttttcaagc agccaaggcc
     1501 gtttcgaaat gttgtcatgc gccgcgtacg cagacccgac cagactgttc cagcgggtga
     1561 cgaaattcgt cccgttttcc gaaacgactt cgaaactgcc ggtcacactc gcgtccatgt
     1621 gcatcagcat cccgtccacc ggaagttcgg tctgcaccgg tttgccggcc aggacaaacg
     1681 ttccgttgga aaccacaacc gacccggaaa gcgcactgag atcggcgatc gaaagaacgc
     1741 tgtcgtcgtt cttcagaaga tcggccgtac ccgtgaaccg ccggatcgat actgccgccc
     1801 cgacgggcag gtcgaccgtc ccgccctcca tgttcagatc gttcatgtcc accctgcccc
     1861 cggcgtaacc cgaggtcggc gcaccaaacc attttcgagc cagatacgct tcgacgtcgc
     1921 ggcgctccac gtcagaaagc aggcgttgat aaataatcac ctctgccagc cgctgtcctc
     1981 cgaaaagctc cacgttttta tgcgtggcaa aggcggaagc gctgcacccg cgatccgtca
     2041 ccaaactcat caactgatat ccgccgttga atccggtcgt acgtccgttc acaaccgcgc
     2101 cgtggattcg cgtcacaccg tgctgcgcca acgattcagg agcccaaatc ggcgaatgcg
     2161 cggagagaat gggagtccgt ttgaagtcgc tgccttccgc agtcgaagcc gttccgagcg
     2221 gcatcccccc gccatcctgc gacccgcaca cccaaaacac ggtccggatg ccgctcactt
     2281 tcgcactcca caaagccgcg cagccgcttt ccgcgccccg gaaggagatc acgggcagcc
     2341 cgttcaacgc atccggcagc aattgcggcg gaattcccgg atccgacggc tgggcggaga
     2401 ggctgccgcc tgacgcgtcc agaatgttgg tgaccgatac cccgtccgca tccagcagaa
     2461 ccgatgccgc atccgaggca tcaacatggt acgcgatacc gccgggcaaa acatacggat
     2521 caggcagaac gcgcgtgtca aaagccagcc gcccctcttt cacgctcaac ggcgccgtca
     2581 accccgcgac ctgtcccacc accagtgtgc ccgcaccggt tttctcgacc ggtccgttcc
     2641 cgtccaaggc gcccagcacc gcctgttggc ctgtgtccac cttcacggcc accgggtcag
     2701 ccgcggaggt gaccaccccg tcaaaggttt gcgaaccgtc ggacgcggca aaacgggcgg
     2761 gtatgttcgg cagccacttg cgcatcagat acccctcgat ctgctcgcgt tcggcagcgt
     2821 ttaagtgccg gttaaagacc agcacttcgg cgatccgttg tccgccgctg cggtattgca
     2881 gcaggcccgg ccgcggatac cgatccgtcg ccatgcaggc ggcccgcgcg gcagaaccgt
     2941 cggtactcac gaaggaaatc aggaacggct cctcttccgg cacgaacgtg gccatctgat
     3001 cgatccggtt cccgttcagg taaacctgac cggtttgcaa cccggccgtc acataagacg
     3061 tattgaaaat gagtccgttc acaccgcggt ggaagttcgc gtagttgccg tcattgatgt
     3121 cggacagcca aaaactttcc ggatgaatat ggtcgaacac catgaacagc gtctggatcg
     3181 cggtgttcgt atgattccag tgaagcagtg cgcctgcacc ccacgcaccg aaatcaacca
     3241 ccggcagccc gttcagtcgc cccggcaccc atcgcggcgc gggattgtcg gcggtgttga
     3301 tggcataggc cagcaaaccg ttggtgacgt tgctgtttcc gcgccactcc aaaacccggt
     3361 cgccgtccag agtcattgag gagaggtcgc tcgcatccag ttggaaatag agaccgggaa
     3421 tgtccatcgc tgtttccagt gtggtgttga ttgcgcgtcc ctggttgacc aacgccagcg
     3481 tgccgccctg cagtgccagc ggacgggaag gccgcgaacc gccgcgctcg acctgcaacg
     3541 tgccgttgcc gccgacctcg cggattcccg aaccgctcag cgtctccaca gcaaaggctg
     3601 cgcccgtctc cacggtcaga cgcgaccggt tactgtccga gacatgcacg cgcagggcgc
     3661 cgggtccggc caccgccatc cctgcatccg cttccatccc ctgccatttt tgccgcagat
     3721 aggcgacggt cgcctcgatc tccgcatccg tcaggatacg gtcataaaag atctcctccg
     3781 ccacctgctg gccaccgaaa cggtacgaac ggtcctgagc gatcgtcccc acggatgtga
     3841 ccccctcttt caccaccatg gcgaccacat cgaattccgt ggcgctcaac ttctgcgaaa
     3901 aggggtcggt caccggaacg ccgttcagcg agatcacata ttccccgttt ttaaaggacg
     3961 cggcagtttc cccccgggaa gtcagcatag tctgatccca acggaacatc cctccggatg
     4021 acagcacaga atggtggtag tgtcccttat ccgtatcggt gaaaatgaaa ttctgggtac
     4081 ggagcacagc gaacgcggcc ttgaccgccg tggatcgcgt attccagatc agatacggcg
     4141 ccagcgaccg ctctgtttta ttcgtcttga tcatgttccc gaagtcgacc accggcaacc
     4201 cattcagcac attcgtcctc acccaaggca aggcgcgctg accttccgtc agatgcccgt
     4261 catgccgaac ataattaccc ggcacttgcg aaaaccactc gaccacaaaa ttcgtgccgt
     4321 tctcttccgc aaaggtgaac gaatccaaat ccgaagcatc aagatgcatg atgggatccg
     4381 cggcaggctt cagcaccggc gacgcggttc cttcaatccg gagatccgca ccgggattgc
     4441 gaatcgtccc gacaaagcgg ttggctgaag cgaccgccac acgcccgcct cccgaaaggg
     4501 aaaggaggcc cgggcccgcc aaatcgtcca caagaagttc cgcaggagcc aaaaatcctc
     4561 cgttcacgac atcgagcccc ccgtagtctt ttacgttcag caccccgaag cgttgttcgg
     4621 tcggcgcggc ggccgacccg agcagacaga agattccgcc tgccgaaagg gtcagatcct
     4681 gatcccggac agacagcggg gcgcggatcg catcgtcctc gccgggcaac gggtccatcg
     4741 cgcgggcatc ggacgtaagc ctcacaccgg atttgacctg cacccatccg gcctccagct
     4801 cattacgccg cgagagttga acgccagaca cggtgtgtgt ctccttcgtc acggtcaatg
     4861 ggttggtggc caccaccggg acatgcagat acgccgtccc gttattggcg atcaacttgg
     4921 gcccggtcgc gtccagcacg atcgcgttcc cgtatatcgc aatattcgtc tgattgaatt
     4981 tcaggacgcc gaccgggcgc gcgccatcgt tcgtgacgct caccgccgca gcagaaaagg
     5041 tcgccgtatg accgcgccca aaggggatca cgccatcctg ccagttgttc gtgtcgctcc
     5101 aaagaccgcc gaccggcgcg atccacgtcc cgctgacatt cgccagaacg gaacccgcaa
     5161 gcccgcacca caagaccatc gcacaatgcc gttttctcat cacaccctcc tcttatcctc
     5221 cgaacgcccg ttcattttgg aaacacatat gccacacggc acccggcggc gcggcgaata
     5281 cgcccgcatt accggatggc ctgtcgtcca atcccgctgc ggaaagcggt cagaagcttt
     5341 tgcttcggac ctcgccgccc ttcatcggga agagaccgac cctttcgggt tccaccagaa
     5401 agatcgcctg atcctcaccg aacttcttgc cgaagaccgg ctccatctca agcaatttgg
     5461 ctttaaccgc cgcgtcaccg cacagcacgg ccctgccgca gaaacgtaga tacgcgctct
     5521 tgtccaccgc cgtgcgcgag atttccacct tcgggttttt cagaagttgc tgatacatcc
     5581 ccttcttggc ggaggtcacg aagagcagct tgttgtccac aatgaacacg tatttgatgg
     5641 gacgcactcg cgggctgtcg ccctcacagg tcgccagata aaagtggcct gctttttcca
     5701 agaaagccgc cgcctcgcgc agcaccggct cctgcttggc cgcgtccccc ttcacccaca
     5761 cgtaactttt cacctcttgt tgcacggccg cagggcaacc cgcatcctgc gccagactgg
     5821 tcagcaccgg catccacagc acccacatcc atttacgcat acggcctttc ctttctttgc
     5881 tgtctgacac gacaccatta aaccatgaaa cattccgcag tttacttgaa ttccacgaga
     5941 aaagaaacac gaaaacacgc attcattttt gattggcgaa aattcagcca atccggtatt
     6001 atccttcata acatcatggt tggcatgcgc tggaaaggga atgggtgaag acatggcgga
     6061 gaaaaagaaa cgggtgggaa tgatcggcgg cggcgaggct tcttttatgg gccccatcca
     6121 ccgcgccgcg attgaacaga gcggctgcgt cgaattggtc tgcggagcct ttgggtccac
     6181 gcggcagagt tcgttcgaga cgggacggat gctgaatctg ccgccccgcc gttcctacgg
     6241 gacttaccgc gacctcttcc ggcgcgaagc ttcgttcccc aaaacggaac gcatcgattt
     6301 tgtcaccgtg ctcacgccga acgcaatgca ctatccgatc gccatgtcct cgatcgacgc
     6361 ttcgttcccg attttcagcg agaaaccctt cacctgtaat ctggatgaag cggtcaacct
     6421 gacccggaaa ctgcggaaca gcggtctcgc ctatggagtg gcgatggtct atccgtgtta
     6481 tcccgccttg cgcaaagcgc aggatctgat ccaaaaagac aaggcgatcg gggtcgtgcg
     6541 caaagtcgtg gccacctttt cattggggtg gatggcccaa cggctggaaa ccgcaggcaa
     6601 caaacaggcc ggctggcgga ctgacccgcg ccggagcgga ccggccggct gtctggcgga
     6661 tctcggatcg cacgcgtttt atctctccga atggctaacc ggcctcagcg tgagcgaggt
     6721 ctgcgccgat ttgcgaccca cggtcgccgg acgcatcctc gatgacgact gcacgcttct
     6781 ggtgcgcttc gagaccggcg cgcgcggcgt attcctgtgc agtcagatcg ccaccggcag
     6841 gcaagagggc ctcgcgatcg aaatctacgg ggataaggcc gcgctcggtt ggtcgcagag
     6901 ccgccccgga cggctgacgc tcaccgcgat tgacgggcaa cgcgaagaac tcaccgtcaa
     6961 cgggtcggat gaggaggagc ccttgcaaac acccccttcg ccctacggca acaaccgcgc
     7021 ctatatcgcc gcactggcgg agtgttaccg ctctttcgcc gcgacgctgg atcgccccaa
     7081 aagccaaaaa acggaaccgc ttcccctcta catgagtgtt gaagaagggt tgcgttcagt
     7141 cgcgtttgtc gatgcggtgc tccagaacac cgccgttccg gaagaggatc agccgctgcc
     7201 agccaaatgg atgccggtgg tggtcccccc tgtactggac ctgtaacgcg cggaaacgcc
     7261 gcgcacagaa cattgccggt ctcccttcac gaacaaacaa accacgccat attcgcttgt
     7321 cttccgtgac aaaaaaacat attgtgtcaa agtgttattt caaacgggac ggcttgtgcg
     7381 tggattcgcg catcaacacg aggggtgcaa catgaccata cagcagggaa aaacgttcac
     7441 cgggttctgt ttgctttcat tctgtgccgc acagggattt gccgccgggt tcggcctcta
     7501 tgaggccagc gcccgcggca acgccatggg cggcgcgctg gtcggcgcca ccggcgacgc
     7561 ctcggccaac tatttcaacg cggccaatct gacggaactg gatggcacgc acacgatggc
     7621 gggtgtgacg ttggtgcacc cggttctgga catcactgcg aacggggaca agaacaatct
     7681 cgacagcggc tggttttatc cgccgcacct ttacgcctcc cagcaactca cggagcactg
     7741 gttcaccggt ttcggcgtct acaccgaata cggcttgggc acgcgctatg accgcgactg
     7801 gccgctcaac tggagcagta ccaaaaccac gctggaaagc ctgaccgtca accccaacct
     7861 cgcttacaag gtgaacgagc gcctctcgct cggcgccgga ttccgtgcga tgtatctgga
     7921 tttctaccat accaagacgc cgtatcagca cgatacgatc ggtgtcctgc ccacgcctct
     7981 gccgccgctg cccgtttacg ggtcgatgcg caccaaagtg gaaggcgatt cttgggggta
     8041 cggctacaat ttagggtttt cctacaaaat caccgacaca ctggacgccg gtttcgttta
     8101 ccggtctcgc gtccggcaca cggtgaaggg cgacatcgaa gtccaaagct ccgccgatac
     8161 cgcgctgccg gtaccgatcc ccaatccgct tccccacggc catgcctcgg cacgcggcga
     8221 gatcgaactg ccgccctcgt ggaccgccgg cctgaactac cggccgatcg accggctcaa
     8281 cctcggattc gccgcgacct ataccgagtg gagttcgtat gatgacctca ccataacctt
     8341 cggccccact ctggcgaaca gcgacaagct gcgccgctcc tccgaggaaa agaactggaa
     8401 tgacgtctgg cgtctgggaa tcggtgccga ctacatgctg acagaacgct ggtccgtcca
     8461 gtgcggctat gtctacgaca tggaccccat cagcaagaat aacaccgaca ccatgctgcc
     8521 ccccggcgac cgccacatca tcagcaccgg cttgggttat gccgtgcaaa cctggacgat
     8581 acatgcgagc tacgggctga ttgtgatgaa atcatgcgaa cgaaccgtca cgaacggcac
     8641 caaacgcgcg gagacggatt tcgataatgg cttttgccat ctgatcgccc tctcggtcgg
     8701 caagcgcttc tgatccactc aggcgcttac ctaacgcagg cgaagcccgc aacccaattc
     8761 aattagttaa ttagttaatt agttaattcg tgcattcgtt ttccatgacc atccgcagtc
     8821 ggttcgtggc cgggatgtgc gaagatcatg cgtttctggt
//
LOCUS       DAOSLE010000114         8832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7476_length_8832_cov_8.096844, whole genome shotgun sequence.
ACCESSION   DAOSLE010000114 DAOSLE010000000
VERSION     DAOSLE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7476_length_8832_cov_8.096844"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PK576_08065"
     CDS             <1..1079
                     /locus_tag="PK576_08065"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOE61585.1"
                     /translation="QAASRGEHMAVFFVNNAIYGMTGGQMAPTTLEGMKTATSPFGRD
                     PLTAGYPLHVCEVLDQLKAPVYIERVSVADTKRIMQAKRAVRKALEIQRDGKGYAFVE
                     FLSPCPTNLGMDANAAALFCVEQMEREYPLGCLRDLQATAQPRPAAGAAPSVEAYFGA
                     STQQSVPEAVIDETFGELRLKFTGYGGQGILSLGVCVAEAARLERRFTTWFPTYGPEQ
                     RGGSAACSVVLSGRPIGSPAVDHPDVLVCMNQPSFERFAKDVKPGGTVIVDATVPQQV
                     PVPEGVRVVTMPAIDLAIKMGVPKAANTMMLAALARTGVTRLQDAHLLAALDASFKNK
                     PALVEKNRLLMREAEAWLDKNAAL"
     gene            1103..1654
                     /locus_tag="PK576_08070"
     CDS             1103..1654
                     /locus_tag="PK576_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE61586.1"
                     /translation="MINEGHEIAVRIRGLREASGHSCQEVATETGVTLATYMRYETGA
                     EDAPLSYLSVLAAFYKVEVTALLTGGDAHARMFHVTRKGGGPVVERRHVYHYEALGAR
                     FAGKAMEPFIVTVGPALETLHLNTHPGQEFNYVLNGRLRVSVGGRETVLEPGDSIYFD
                     ASVPHGMRAEDGVEATFLAVITA"
     gene            1670..3340
                     /locus_tag="PK576_08075"
     CDS             1670..3340
                     /locus_tag="PK576_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE61587.1"
                     /translation="MREAWLNQTTFNSYEAFYREFHLNVPECFNFAFDVVDAIAAEDP
                     ERRAMVWCDDQGSERVFTMSDIARASNQAARAFATAGIRRGDAVMLILKRRYEYWFAL
                     MGLCRLGAVAIPATNQLTQKDVVYRIRSAGVSMILCVNEPAIRAAVDAAVTEAPVRVR
                     ALVNGGDDGTGWMDFNAACDQASPEPLEAALCARDTGACDPMLLYFTSGTTGMPKMVQ
                     HDFLYPFGHVVTARYWQGCTPGGLHLTLAETGWAKAAWGKIYGQWMCGCAVMVYDMDR
                     FHAEAVLRIVERYRVTTFCAPPTIYRYLIKEDLKRCDLSALTRCVVAGEPLNPEVYQQ
                     FLDATGLKLYEGYGQTEVTVAAGTFPWMKPKPGSMGCAAPGYDLDLIRHDGRPCEVGE
                     EGQLVIRTERSKPLGMFNGYYRDPELTHQMWRDGVYYTGDMAWRDEDGYFWFVGRADD
                     VIKSSGYRIGPFEVESALLEHPAVLECAVTGVPDPDRGQVVKATVVLAKGFEASDALA
                     SALQEHVKRITAPYKYPRRVEFVSELPKTISGKIRRVQIREQDQRNAE"
     gene            complement(3486..7217)
                     /gene="smc"
                     /locus_tag="PK576_08080"
     CDS             complement(3486..7217)
                     /gene="smc"
                     /locus_tag="PK576_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOE61588.1"
                     /translation="MYLKQIELIGFKSFAEKTRLQFEPGMIAIVGPNGCGKSNISDAI
                     RWVLGEQRPTALRCAKMADVVFNGTDSRKPLGMAEVSITFADCEGFLETEFNEVTVTR
                     RVFRSGEGQYFINKTPCRLKDVQRLFMGTGIGTTSYSVMAQGQIDIILSSRPEDRRIV
                     FEEAAGITKFKADRKEAMRKIDQTDANLLRLADVIREVKRQIGTLQRQAGKAQKYKEL
                     RDELRGLDIFLTRRRLAALDIRIRELDTTIHALHEQWSSHQEFVAEAEAESSRIHGLI
                     HETEERIANLTEQATQADNRYLRAQEVIKVNEQRIAEYRAWAERDNREISETRTQIEQ
                     LRLQQESLTQKRLLLQQNEEVEQKALKEAQTQFDAHRLQIEQTRAQLQQDRQKSVECE
                     RRTAQIQQELADMEARQRELLMKRDRLAAEHGQMQETLAATEQACAEVQARLDTLTAE
                     VAATDERFEARNAEQAAVAEELNTLQAESARLQSEAAAKRAQIELLKDAGEARDDYAP
                     GSQLLLDPANPLGLEPQTVLGPLAEMFNAPPDLRLALEAALRAWLDAVIVRDAESARQ
                     IFALLLARGTPAAARVIAASSQGLSGGVDPDAPLSPPSPGLTPLLDRVTVEEPFSEAA
                     RRLLGTVFLAESLDAVPAVLPAGCRVVTRDGVIAHADGCAELWMPDSPVSSPLARKLL
                     AAEAADQLAVLETTFRTTRTRQEQLAARSAELSLNLTQMRKTLDENRRRAAQAEGEHQ
                     SVSRDAERARARLDHVSRELGALNEQTSGDEEIRAAHTNELDELTVMRASLLERITEN
                     STLLQEREGVFSELSHTLTECRIRVSSVSQQIEHAVGQREAIQTRMDELERTVQGRNR
                     GVMSYDESIERLAQEIASLEANLDPMRITAETLHQKIEETRRERAARQSDLEKTDATL
                     TERRRELETVREQRSRAEIDLAESRIRRQNHLDHVYNEYGLSPEELIAHADPAWKEGG
                     PPPISEIEARTTSLNADIQALGPVNLVAIEEHKELEERYSFLKAQEEDLLKSKEQILE
                     LIAMINKKSSEMFQSTFDQANAHFSTMFTRLFNGGQAKLVLLENAEDPLECGIDIIAR
                     PPGKRPQSITLLSGGERTMTAVSLLFAIFMIKPAPFCMLDELDAALDDSNIGRFVQTL
                     KDFLKHSQFLIITHNQHTIASSDIVYGVTQQEKGISKIVSMRLKEIGIKPLELGVPED
                     AANAVSAVEEPPPRSRRRGRKKKSGDDAAPDPEVSAPAE"
     gene            7468..8706
                     /locus_tag="PK576_08085"
     CDS             7468..8706
                     /locus_tag="PK576_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61589.1"
                     /translation="MNRCWVLLGLIVVLGHAANGAIEIACDYPGGNVRVIDIDEKSGV
                     VKVAPDLRDTVGNWFHFDFTVTGADGKKLSFRFPEGGFPYISSLGPAISADGGRSWRW
                     LNQDGRRHEPANRFDYVFDEKESSVRFAMSIPYSQKDWDDFMKAYRENPDVKFETLCK
                     SQSGQRDVELVRVPCRGKARYLMVLTARHHACETTGNPPMEGAFAELMSGSKDGNWLR
                     DHADCVFVPFMDKDGVEAGDQGKNRKPWDYNRDYLKGRYTSVRALKDLIVRDSVGKRI
                     VFLDLHSPFVRSFKECPEQDQVFSFGLTHALQNARWNDFRRNWIETQKGGGLRYTGAY
                     DIQAGTGHAAITEKAVREGFAGSHHWVSMLENCCLSTCVEFGYSLCGGVNSIAGMREL
                     GMNMMKAVARTVSIEPKRSN"
BASE COUNT         1581 a         2472 c         2907 g         1872 t
ORIGIN      
        1 tccaggcggc gagccgcggc gagcacatgg cggtcttttt cgtcaacaac gcgatttacg
       61 gtatgaccgg cggacagatg gcgccgacca cgctggaggg gatgaagacc gccacctcgc
      121 cgttcgggcg cgatccgctg accgccgggt atccgctgca cgtctgcgaa gtgctcgatc
      181 aactgaaggc cccggtctac atcgagcgtg tatcggtggc ggacaccaag cggatcatgc
      241 aagccaaacg tgccgtgcgt aaggcgctgg agatccagcg cgacgggaag gggtacgcgt
      301 ttgtcgagtt cctctcgccg tgccccacca atctgggcat ggatgcgaac gcggccgcgc
      361 ttttctgtgt ggagcagatg gagcgggaat atccgctggg atgcctgcgc gacctgcagg
      421 ccacggcgca gccccgccca gccgcgggcg ccgcgccctc ggtcgaagcc tatttcggcg
      481 cgtccacgca gcagtcggtg ccggaggcgg tgatcgacga gacattcggc gagttacgcc
      541 tcaagttcac gggctacggt ggtcaaggca ttctgagcct cggtgtctgt gtggctgaag
      601 ccgcacggct cgaacggcgt ttcacgacgt ggttccccac gtacgggccg gaacagcgcg
      661 gcggttcggc cgcctgttca gtggtcctct ccggacggcc gatcgggtca cccgcagtag
      721 atcatccgga tgtgctggtg tgcatgaatc aaccctcttt tgaacggttt gcgaaggatg
      781 tgaaacccgg cgggacggtg atcgtggatg cgacggtgcc gcagcaggtt ccggtgccgg
      841 agggggtgcg tgtggtcacc atgcctgcga tcgatctggc aatcaaaatg ggtgtgccca
      901 aggccgcgaa caccatgatg ctcgcggctc tggcccggac gggcgtgacc cgtctgcaag
      961 acgcacatct gttggcggcg ctcgatgcga gtttcaagaa caagccggcg ctggtcgaga
     1021 agaaccgtct gttgatgcgt gaagccgagg cctggctgga taaaaatgcg gcgttgtaac
     1081 ggtgccgcag aatggggggg gtatgataaa cgaggggcat gagatcgcgg tgcggatccg
     1141 ggggttgagg gaggcatcgg ggcattcgtg tcaggaggtc gccaccgaaa cgggggtcac
     1201 gctcgcaacc tatatgcgat atgagaccgg ggcggaagac gcgcccctga gctacctttc
     1261 ggtgctggcc gcattttaca aggtcgaggt cacagccctg ctcaccggcg gcgacgcgca
     1321 cgcacggatg ttccatgtga cgcgcaaggg cggcggtccg gtggtcgagc ggcggcacgt
     1381 ctaccattat gaggcgttgg gcgcacggtt cgcgggtaaa gccatggagc cgtttatcgt
     1441 gacggtggga ccggcgctgg agaccctgca tctcaacacc catcccggtc aggagttcaa
     1501 ttatgtgctg aacggccggt tacgggtttc ggtgggcggt cgtgaaacgg tgctggaacc
     1561 gggggacagc atctattttg acgcttcggt gccgcacggc atgcgggctg aggacggtgt
     1621 ggaggccacg tttctggcgg tgatcaccgc ataagcggag gggagacgta tgcgggaagc
     1681 gtggctcaat cagacaacgt tcaattcgta tgaggcgttc taccgggagt ttcacctcaa
     1741 tgtgcccgaa tgtttcaatt tcgcctttga tgtggtggat gcgatcgcgg cggaggatcc
     1801 ggaacgacgc gcgatggtct ggtgcgatga tcaagggagt gagcgggtct tcacgatgag
     1861 cgacatcgcg cgcgccagca accaggcggc ccgtgcgttc gcgacagcgg gcatccgccg
     1921 cggggatgcc gtgatgctga tcctgaaacg gcggtatgag tattggttcg cgttgatggg
     1981 actgtgccgc ttgggggccg tggcgatccc ggccaccaac caactgaccc agaaggatgt
     2041 cgtgtaccgc attcgctcgg ccggagtcag catgatccta tgcgtcaacg aacccgcgat
     2101 ccgcgcggcg gtggatgcgg cggtcacaga agcgccggta cgcgtacggg cattggtgaa
     2161 tggtggggac gacggcacgg gctggatgga tttcaatgcc gcatgcgatc aggcttcgcc
     2221 ggagccgctg gaggcggcgc tctgtgcgcg ggacaccggg gcttgtgatc cgatgctgct
     2281 ctactttacc tccggcacga ccggcatgcc gaaaatggtg cagcacgatt tcctgtatcc
     2341 ctttggacat gttgtgacgg cccggtattg gcagggttgc acaccgggcg gcttgcacct
     2401 gactctggcg gaaaccggat gggccaaagc cgcgtggggt aagatctacg gtcagtggat
     2461 gtgcggttgc gcggtcatgg tatatgacat ggaccgtttt catgctgagg cggtattgcg
     2521 gatcgtcgaa cgctaccggg tcaccacatt ctgtgcgccg cccaccatat accggtatct
     2581 catcaaagag gatctgaaac gctgcgattt gtcggcgctc acccggtgcg tggtggccgg
     2641 cgagccgctt aaccccgaag tctatcaaca gtttttggat gccacggggc tgaaactgta
     2701 tgaggggtac ggtcagacag aggtgaccgt ggcggcaggc accttcccgt ggatgaagcc
     2761 caagcccggg tccatgggct gtgccgcgcc ggggtatgac ttggacctca ttcgtcatga
     2821 cggccgaccg tgcgaagtgg gcgaggaagg gcaactggtg atccgcacgg aacgctccaa
     2881 gccgctcggt atgttcaatg gctactatcg cgatcctgaa ctgacgcatc agatgtggcg
     2941 ggacggggtc tattacaccg gcgacatggc ttggcgcgat gaggacgggt acttctggtt
     3001 tgtaggacgg gcggatgatg tgatcaagag ttccgggtat cgcatcggac cctttgaggt
     3061 ggaaagcgcg ctgttggagc atcccgctgt actggaatgc gcggtgacgg gtgtgccgga
     3121 tccggaccgc ggtcaggtgg tgaaggccac ggtcgtgctt gccaaaggat ttgaggcttc
     3181 cgacgcgctg gctagcgcgt tgcaggagca tgtcaaacgc atcacggcgc catacaaata
     3241 tccgcgccgc gtcgagtttg tttcggagtt gccgaaaacg atcagcggca agatccgccg
     3301 cgtacagatt cgggaacagg atcaacgcaa cgcggaatag ccgacgcaag cttccgccaa
     3361 gtttatcgcc attgccgtcc gctatggcgc gggcgatccg tgggagatta tggaagggcg
     3421 cgcggcgctc acggagcgca tggccgaggc agtcctcgat ctcggcgcac aagaggttgc
     3481 cgggattatt cggctggcgc gctgacttcg gggtcggggg cggcgtcgtc cccgctcttc
     3541 tttttgcggc cgcgccgccg actccgcggc ggcggctctt ctacggccga aaccgcgttc
     3601 gccgcatcct cggggacgcc cagttccagc ggtttgatcc cgatctcctt gagacgcatc
     3661 gagacgatct tcgagatgcc cttctcctgc tgtgtcacgc cgtaaacgat atccgaactg
     3721 gcgatggtgt gctggttgtg cgtgatgatc aggaactgcg agtgtttcaa gaagtctttc
     3781 agggtctgaa cgaaacgtcc gatgttgctg tcatccagcg cggcgtccag ttcgtcgagc
     3841 atgcagaacg gggccggttt gatcatgaaa atcgcgaaga gcagactcac cgcggtcatg
     3901 gtgcgttcgc cgcccgagag cagggtgatc gactgtggtc gtttgccggg cggacgggcg
     3961 atgatgtcga taccgcattc aagcgggtct tcggcgtttt ccagcaacac gagttttgct
     4021 tggccaccat tgaagagccg agtgaacatc gtgctgaaat gggcgttggc ctgatcgaag
     4081 gtggattgga acatctcgct cgacttcttg ttgatcatgg cgatcagttc cagaatctgc
     4141 tctttggact tgaggagatc ctcctcctga gccttgagga aggagtaacg ttcctccagt
     4201 tccttgtgtt cctcgatggc caccagattt acagggccga gcgcctgaat gtcggcattg
     4261 agggaagtcg tgcgcgcctc gatttcggag atcggcggcg gtccgccctc cttccaggcg
     4321 ggatccgcat gggcgatcag ttcctcgggc gagagtccgt attcgttgta gacgtggtcc
     4381 agatggttct ggcgtcgtat ccgcgattcg gcgagatcaa tctccgcgcg gctgcgttgt
     4441 tcgcgcacag tttcaagttc gcggcggcgc tcggtcaagg tggcgtccgt cttctccaga
     4501 tcactctggc gggcggcgcg ttcgcggcgc gtctcctcga tcttttgatg aagcgtttcg
     4561 gccgtgatcc gcatggggtc gaggttcgct tccagggagg cgatctcctg cgcgaggcgc
     4621 tcgatgcttt catcgtatga catcacgccg cggttccggc cctgcaccgt gcgctccaac
     4681 tcgtccatcc gtgtctggat ggcttctctc tggcccaccg cgtgctcgat ctgctgcgac
     4741 acgctggaaa ctcggatccg gcactcggtg agggtgtggc tgagttcgct aaaaacgccc
     4801 tcccgttcct gcagcagggt cgagttttcg gtaatgcgtt ccaacaagct tgcgcgcatc
     4861 accgtgagtt cgtccaattc gtttgtgtgc gcggcacgga tttcctcgtc cccggaagtc
     4921 tgctcgttga gtgcgcccaa ctcacgggat acgtgatcca gacgggcgcg ggcccgttcc
     4981 gcgtcacggg aaacgctctg atgctcacct tccgcctgcg cggcgcggcg ccggttttca
     5041 tccagcgttt tccgcatctg cgtcaggttg aggctcagtt cagccgaacg cgccgccaac
     5101 tgctcttggc gggtccgggt ggtcctgaac gttgtttcca gcacagcgag ctggtcggca
     5161 gcttctgcgg cgagcagttt gcgggcgagc ggactggaga ccgggctgtc cggcatccac
     5221 aactccgcac atccgtcggc atgcgcgatc acgccatcac gcgtgacgac acggcacccg
     5281 gcgggcagaa ccgcaggtac ggcatccagc gactccgcca aaaagacggt gcccaacagg
     5341 cggcgcgccg cttcggaaaa cggttcttca acggtgacgc gatccagcag tggcgtgagg
     5401 cccgggctgg gcggagacaa cggcgcatcc ggatccacgc cgccggatag gccttgggac
     5461 gaagccgcga tgacacgcgc ggctgcgggg gtaccgcgtg cgagcagcag cgcgaagatc
     5521 tggcgcgcgg actcggcgtc gcgcacgatc acggcatcga gccaagcgcg cagggcggct
     5581 tccagcgcaa ggcgcaggtc cggcggcgcg ttgaacatct ctgccagcgg gccgagcacg
     5641 gtttgtggtt cgaggcccag cggattggcg gggtccagca agagttgcga gcctggcgca
     5701 tagtcgtcgc gggcctcgcc cgcgtctttt agcaactcga tctgcgcccg cttggccgca
     5761 gcctcggatt ggaggcgcgc ggattcggct tgcagcgtgt tcagttcttc ggctacggcc
     5821 gcctgttcgg cgttgcgtgc ttcaaaccgt tcgtctgttg cggcgacctc ggccgtcaac
     5881 gtgtcgagac gagcctggac ttcagcgcaa gcttgttccg tggccgccaa cgtctcttgc
     5941 atctggccgt gttcggcggc aaggcggtcc cgcttcatga gcagctcgcg ttgccgcgcc
     6001 tccatgtccg ccagttcctg ctggatctgg gcggtgcggc gttcgcattc taccgacttc
     6061 tgccgatcct gctggagctg tgcgcgcgtt tgttcgatct gaagccggtg cgcgtcgaat
     6121 tgggtttgcg cctcttttag agctttctgt tcgacttcct cattctgctg caagagcaag
     6181 cgtttttgcg tgagcgattc ctgctgcaag cggagttgtt cgatctgcgt ccgggtctcg
     6241 gaaatctcgc ggttgtcgcg ttccgcccaa gcgcggtatt cggcgatgcg ctgctcgttg
     6301 accttgatga cttcctgggc gcggaggtaa cggttgtcgg cctgtgtcgc ttgttccgtc
     6361 aggttcgcga tccgctcttc ggtctcatgg atcaatccgt ggattcggga ggactccgcc
     6421 tcggcctccg ccacgaactc ctgatgtgaa ctccactgct cgtgcaacgc atggatcgtg
     6481 gtgtccaact cacggatgcg gatatcaagc gcggcgagac gccgacgcgt caggaaaatg
     6541 tcgaggccgc gcaattcatc gcgcagctct ttgtattttt gggctttacc ggcctggcgc
     6601 tgaagcgtgc cgatctggcg cttcacctcc cggattacat cggcgagccg cagcaggttg
     6661 gcatcggtct gatcaatctt gcgcatcgcc tctttgcggt cagccttgaa tttcgtgatg
     6721 ccggccgcct cctcgaaaac gatgcggcgg tcttccggcc gcgaggagag gatgatgtcg
     6781 atttgtccct gggccatcac cgaataggag gtcgtgccaa tgcccgtccc catgaagagc
     6841 cgttgaacgt ctttcaggcg gcagggggtc ttgttgatga agtactggcc ttctccggac
     6901 cggaagacgc ggcgtgtgac agtcacctcg ttgaattcgg tttcgaggaa cccttcgcaa
     6961 tccgcgaagg tgatggagac ctccgccata ccgagcggct tgcgcgaatc cgtgccgttg
     7021 aacaccacat ccgccatttt tgcgcaacgc aaggcggtgg gacgctgttc acccagcacc
     7081 cagcggatcg cgtccgagat attacttttg ccgcagccgt tgggtccaac gatcgcgatc
     7141 atgccgggtt cgaactgcag acgggtcttt tccgcaaagc tcttgaaacc aatcagttca
     7201 atctgtttta gatacacgaa aaacctcagg gagccagatt gacggcggga tgcctgtcag
     7261 ccgtcacaga aagaggtact tatatcaaaa cagggggccg gattcaaagc ctaaagcagt
     7321 ttttcacctg cttgttggga gaggtgtgtc caacgcttgc tttttcggat taaattgcaa
     7381 tactgttcat tttcttgctt aattgattgt cgaggaaaat tgtggacgtc aaggtccgtc
     7441 tgttttaata atagaggaag gagtgacgtg aataggtgtt gggtactgtt gggtctcatc
     7501 gttgtgcttg gacatgcggc gaacggggcg attgagattg cgtgcgacta tcccggcggg
     7561 aacgtgcgcg tcattgacat cgacgagaag tccggcgtgg tgaaggtcgc tcctgacttg
     7621 cgggacaccg tgggcaattg gttccatttc gatttcaccg tgacgggcgc ggatgggaag
     7681 aagctgagtt ttcgctttcc ggaaggtggc ttcccgtaca tctcctcact gggaccggcg
     7741 atcagcgctg atggtggacg cagttggcgc tggttgaacc aagacggacg gcgacatgag
     7801 ccggcaaaca ggttcgatta cgtcttcgac gaaaaagaat cgtcggtgcg tttcgcgatg
     7861 tcgattccgt attcccagaa ggactgggat gacttcatga aggcctatcg cgagaatccg
     7921 gacgtgaagt tcgagacgct ttgcaagtcg cagagtggtc aacgggatgt tgaattggtg
     7981 cgggttccct gtcgcggaaa ggcccgctat ctgatggtgc tgaccgcgcg ccaccatgcc
     8041 tgcgagacca cggggaatcc gccgatggag ggagcgtttg ccgaactgat gtccggatcc
     8101 aaagacggga actggctgcg cgatcatgcc gactgcgtat tcgtgccctt catggacaag
     8161 gacggtgtcg aggcgggcga ccaaggcaag aatcgcaagc cgtgggatta caatcgggac
     8221 tatttgaagg ggcgctacac gtccgtgcgg gcgcttaagg atctgattgt cagggattct
     8281 gtcggcaaac gcattgtctt tctcgatctc cattcgccgt tcgtgcggag cttcaaggag
     8341 tgtcccgagc aggatcaggt attctcattc ggcctcaccc atgcgttaca gaacgcccgc
     8401 tggaacgact tccggcgcaa ctggatcgag acgcagaagg gcgggggact gaggtatacc
     8461 ggcgcctacg acattcaggc tggcaccggc catgcggcta taaccgagaa ggccgttcgc
     8521 gaagggttcg ccggttcgca tcattgggtc tcgatgcttg aaaattgctg cctctcgaca
     8581 tgcgtcgagt ttggctattc gctctgcggc ggcgtgaact cgatcgccgg tatgcgcgaa
     8641 ctcggcatga acatgatgaa ggctgtggcg cggacggtat ccattgaacc caaaaggagc
     8701 aattgaacgg gtagaattct agcaaatctc cgtaaacatt gggttttgcg gtgcgcccga
     8761 cgctcttttt gaactcttcc cgtcagacct tatgtcgacc cgcgacccgc gacgagcgac
     8821 ctgcggtgtc gg
//
LOCUS       DAOSLE010000115         8812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7496_length_8812_cov_7.988695, whole genome shotgun sequence.
ACCESSION   DAOSLE010000115 DAOSLE010000000
VERSION     DAOSLE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7496_length_8812_cov_7.988695"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="PK576_08090"
     CDS             complement(<1..2008)
                     /locus_tag="PK576_08090"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HOE61590.1"
                     /translation="MMMRFRPIFLSLLCVSVFSGVRADPAVVPIGMAGGAAGAVVPLP
                     QGLPVAFPGEVNVPLASTVLEPWLKADALHLAVAWPEGKPERVGALVWLKDRDAFWHQ
                     YLIPQSGEGKETNLWVIPFRPGAAGWQTPGHTLAWHHRVRMSPQSVGVRLFGDSVFTG
                     KCVLVSAFLTPQPSASPPEIVKVRPLTRSARAYALYEARFDLPDRYDNPFDPGQIDAG
                     AVVITPAGQTNAIHAFYYQPHYRLEDELGQPVEPDGQPEWRIRYCPREAGEYRVSLIA
                     RDAHGEVTLTNALRFHAAPADPEAMRFIRVSQKDPRFFELDDGRLFYPIGHNVRSATD
                     SRMDDKFPWVFRQDEGPTAYRRYFSRMQANGENIAEVWMSAWSLGLEWTEGVSGYHGA
                     NDYHMGNAWELDRVIDLAHEHNLYINLVLNYHGRISTWCDPEWHLHPYNKATPGGWLT
                     RPLEFFSDERALEMQRRFCRYTQARWGWAPAIFGYELCSEINLTGHETHHKTHFDPAV
                     VAWCRALGGYLKRIDVYGHLVSAHVSNDYTFLNPAISNLTEMDFNPLDAYHHGQPERI
                     IPLVAATADAGAAYNKPILITEFGGSPMAAGREHLLIEQHAALWAGACVPMSGVPMFW
                     WWQVIDENDLYGRYAAIQTFMTGIDPRNALAKQVKPALGVSENGD"
     gene            complement(2090..2257)
                     /locus_tag="PK576_08095"
     CDS             complement(2090..2257)
                     /locus_tag="PK576_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61591.1"
                     /translation="MGETTGLRDTETGSPDSFGRYIMSKNHDAKKEAKKKPTKTLKEK
                     RLEKKAKKTAQ"
     gene            2411..3244
                     /gene="recO"
                     /locus_tag="PK576_08100"
     CDS             2411..3244
                     /gene="recO"
                     /locus_tag="PK576_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE61592.1"
                     /translation="MIIRTPALALQIRPWSQTSQMVTWLTPGHGKIVTAVKGACRPKS
                     AFLGQYDLFYTCDLLFYRRDRGGVHAIRECSPVTLRSALRQNWRHATAAAYLADLTAQ
                     TAVGHQETTALFELLTCALDHLTTARCVPRELIVWYETHLLRLLGLLPDFTTCPLCHP
                     PERRWMRFSLPSGRFVCPHLAQHHPDDAVITLHRGVPALFHRFSRLGTPPADNMKHQP
                     TVPPPSPPPENLLLGLCRFLGIFIPFHLDVPSVVRRVTFEMIDTQHICGTCAALREIG
                     S"
     gene            3241..4344
                     /locus_tag="PK576_08105"
     CDS             3241..4344
                     /locus_tag="PK576_08105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE61593.1"
                     /translation="MMKCSPILLCTALTTTLFLTGGCKKEATGQPAANMDAAVAAAAQ
                     TPETSTDPNEVVASVNATRYLRKDMDQTVSALLKAQNIPEAQLEDARKHFEQRVVYGF
                     IMKTLLLDEAKKQTVTVTEEDRKAQIDKIAEALKPQNKTTEQYFKDSPLGEKVARAEF
                     EDGLVIDKLLRKTVLDSIVIDEEEVKKTLAEIEARNAEIAATNKNLIAINAERKAKIQ
                     ELKKQLDNGADFAELAKAHSECPSKEQGGDLGPFTAGQMVKPFEDAAFAQEINKVGDI
                     VETSFGYHLIKVTAKSPATEAKGDTPAKPASVTASHILIKTESPQELQEVPTSEQIQN
                     HLKQQKSRAAVEKYIEGLKAAAKIETIYKEIPL"
     gene            4500..5654
                     /locus_tag="PK576_08110"
     CDS             4500..5654
                     /locus_tag="PK576_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOE61594.1"
                     /translation="MSVKVLMLDLVSEWGGAQHSLYDACVSLSRLGVEPVAAVPQGPL
                     FDRLTTAGITVFPVSPLRAHRRGFALFTTAAKLLRAPATVSQILKVVKPDIIHCNSLQ
                     AFLAVRGHHAKTPLIWHVRDLKMPVALARDAAKKAARLFAVSEAVDEYLVEILPQKHL
                     GRIRVVRNGIDLQRFGQTCRVEARSRFGLPPDAPIVGMIAHLAPWKRHDAFIRTAARI
                     RARQPDAHFVSVGRDLFGENTRWRTQLNAEVEKERLSECFHWVTDLDQAETILPAFDV
                     LVHPALREPFGRVICEAMASGVPVVAAESGGPATLIRQQVTGVLVRDGDEEGMADAAL
                     ALLADRVRASQIADAARAFVQEHHAIEQTAEQLLKEYHSLLASETGRYET"
     gene            complement(5664..7010)
                     /locus_tag="PK576_08115"
     CDS             complement(5664..7010)
                     /locus_tag="PK576_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61595.1"
                     /translation="MIALARKIKARLGRLWWYTAILFLVLRACDLCNAFIGLWLVPRY
                     VPQDELGAVLPLTQVGGVLGLPLAILLVPFTKFLNTYATRGEYGKVKRLLRDVFVLSL
                     LLFVGTLCFARFILPLVFERMRVGAGSLGLLIVASGVLSVLSPLFINALQALQQFRMV
                     AVINLIAAPIRLLAMWIAMPFRALSGYFVGQSAPSLFLIGASLFGLRRHLGREVRSEP
                     YWQKDWHSILRYTLPVAMMTVANAVQTAVESFVIRHRLSELDSAGYYVISRFAEIGAY
                     VGLTFMFVLFPLAAERHERGKRSDRYLWHAIGGTLVSGLILTGLFALFGEWLLRLNPV
                     WSDYAGYDHAMVVLTLVVVARTAASCFTSHEMACGRFGFVCGFSLIAGIECAVLYGLT
                     GVSFFYGILPTSWVDGLASLQAGRLEFILNVMLASAVALCAYVGLHEFRRRRQTTG"
     gene            7442..>8812
                     /locus_tag="PK576_08120"
     CDS             7442..>8812
                     /locus_tag="PK576_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOE61596.1"
                     /translation="MKRRIFLSAAALAVAGLACITMTFCAHAADTPSALEKNGRAKPQ
                     VFHLDLAFAKYRPDYLRRLIPQLKKCGFNTILWEMEDAVRFDTCPEIAAPDAMSKAEL
                     AAILAFARESGLENMPLLQTLSHNAYVLRHPKYQHLSEKPGFLELYCLSRPETGRFLT
                     DWAREIADLFGNPRYFHIGADEAHKIGKECDLCRKSVEKLGVAGLIVKHLSPVKQALF
                     ERRITPVMWADMVHNHPELKTMLDRRTILADWWYESAERAFRQVGPEKKRVNVFAEAA
                     ELRKSGFAVWGCAASRSWGDSVFAPRHDHHLKNYREWRRAFAEIPLEGVMLTSWSWHL
                     FPLELQMPVVALAAYDGLDDAQYEEAFARDWLGIDSKEFFRAARLLSTPVLFSMTAKV
                     GHNQVNHVAAPDAVMKEALKLSPKEKDRARESEKAAALLAGYQDAASALRKLEAQAGS
                     DKEMLRD"
BASE COUNT         1919 a         2823 c         2483 g         1587 t
ORIGIN      
        1 ggtccccgtt ttccgacacc cccagtgccg gcttcacctg cttggcaagg gcattgcggg
       61 gatcgatacc ggtcatgaag gtttggatcg ccgcataccg tccgtacaga tcgttctcat
      121 cgatcacctg ccaccaccag aacatcggca caccggacat cggcacacag gcgccggccc
      181 acagcgcggc atgctgttca atcagaaggt gctcgcgtcc cgcggccata ggcgacccgc
      241 cgaactcggt gatgaggatc ggcttgttgt acgccgcgcc ggcgtcagcg gtcgccgcca
      301 ccagcgggat gatgcgctcg ggctgcccgt gatgataggc gtccagggga ttgaaatcca
      361 tctccgtcag attagagatg gcagggttga gaaacgtgta atcgttggac acatgcgcgg
      421 aaaccagatg gccatagaca tcgatgcgct tgagatatcc gccgagggca cggcaccagg
      481 cgacgaccgc cgggtcgaaa tgcgttttat gatgggtttc gtgccccgtg aggttgattt
      541 cactgcacaa ttcgtacccg aaaatcgcgg gcgcccaccc ccagcgcgcc tgcgtgtagc
      601 gacagaaacg acgctgcatc tccagcgccc gttcgtcgct gaaaaattcg agcggccggg
      661 tgagccaacc tccgggagtc gccttgttgt aaggatgcag atgccattcc ggatcgcacc
      721 atgtgctgat acgcccgtgg taattcagca ccaggttgat gtacaggttg tgctcgtggg
      781 cgagatcgat cacacggtcc agttcccatg cgttgcccat gtggtaatcg ttcgccccgt
      841 gatacccgct cacgccttcg gtccactcca atcccagcga ccacgcgctc atccagactt
      901 cggcgatgtt ctcgccgttc gcctgcatgc ggctgaaata acgtcgatag gcggtcgggc
      961 cctcatcctg ccggaacacc caggggaact tatcatccat ccgcgaatcc gttgccgagc
     1021 gcacgttgtg gccgatcgga taaaagagcc gtccgtcatc caactcgaaa aagcgcgggt
     1081 ctttttgcga gacccggata aagcgcatcg cttccggatc agccggagcg gcatggaacc
     1141 ggagggcgtt cgtgagcgtc acctcaccgt gcgcatcgcg cgcgatcaga gagacccggt
     1201 attcacccgc ttcgcgcgga cagtaccgga tgcgccattc gggctggccg tccggctcaa
     1261 ccggctggcc gagttcgtcc tccagccggt aatgcggctg atagtagaag gcatggatcg
     1321 cattcgtctg gcccgcgggt gtgatcacga ccgcacccgc gtcaatctgg ccgggatcga
     1381 aggggttgtc atagcggtcg ggcagatcaa accgcgcttc gtacagggca taggcgcgcg
     1441 cggaacgggt cagcggccgc accttcacaa tctccggcgg cgacgcggac ggctgcggcg
     1501 tgaggaatgc cgacaccaga acgcacttgc cggtaaagac cgagtctccg aaaaggcgga
     1561 cccccacgct ctgcggactc atccggacac gatggtgcca agccagcgtg tgccccggcg
     1621 tctgccatcc cgcggccccg ggccggaacg gaatgaccca caggttggtc tccttcccct
     1681 ccccgctttg cgggatgaga tactggtgcc agaacgcgtc acggtctttg agccaaacca
     1741 acgccccgac ccgctccggt ttcccttcgg gccaagccac ggccaggtgc agtgcatcgg
     1801 ccttcagcca gggttcaaga accgtcgacg ccaacggcac attcacttca ccgggaaaag
     1861 ccacgggcaa gccctgcggc aacgggacaa ccgcaccggc cgctcccccc gccatcccga
     1921 tcgggacgac ggcaggatcg gcgcgcactc cggaaaaaac agacacgcag agcagagaga
     1981 gaaaaatcgg gcggaaacgc atcatcatgc cggattaaga agcacccccc atgccagaaa
     2041 acaagggggg ggaaatgcgc tccagacaaa accggagcgc gcgaaaaggt cactgtgccg
     2101 ttttcttggc ttttttctcc agacgcttct ctttcagcgt cttggtcggt ttctttttcg
     2161 cttctttttt cgcatcgtga tttttggaca tgatgtacct cccgaacgaa tcgggcgaac
     2221 cggtttccgt atcgcggagc ccggtcgtct cgcccaagtt ggccgcacct taacaggttg
     2281 cctcccccct tgtcaaccgc tcgccgtcaa ttcttctttt accgggaaaa cccgacgggt
     2341 gccccaccct gcgggttacg gcttgtctac cgcgccaaaa gaggcgatac tccccccata
     2401 tccgacacgt atgattatcc ggacaccagc ccttgccctg caaatccgcc cttggtccca
     2461 aacctcgcag atggtgacct ggctgacacc gggtcacggc aagatcgtga ccgccgtgaa
     2521 aggggcctgc cgccccaaga gcgcctttct gggacagtat gacctctttt acacctgcga
     2581 cctgcttttc tatcgcagag accgcggggg cgtgcatgcc atccgcgaat gttcgcccgt
     2641 caccctgcga agcgcgttac gccagaattg gcgccatgcg acggccgcgg cgtaccttgc
     2701 cgatctgacc gcccaaacgg cagtcggcca tcaggagacc acggcgctgt tcgaactgct
     2761 gacttgcgca cttgaccacc tcacgacggc gcgatgtgtc ccgcgcgagc tgatcgtttg
     2821 gtacgagacg cacctgctcc gtctgttggg actgttgccg gacttcacga cctgccccct
     2881 ctgccacccc cccgagcggc gctggatgcg attctccctc ccctccggac gctttgtctg
     2941 cccgcacctc gcccagcacc atccggacga tgccgtcatc acgctgcacc ggggggtccc
     3001 cgccctcttc catcgcttca gccgactcgg gaccccaccc gccgacaaca tgaagcatca
     3061 gccgaccgtg ccccccccct cccctccccc tgaaaatctt ctccttggct tatgccgatt
     3121 tcttggtatc tttatcccgt ttcacctcga tgtgccttcg gttgtccggc gtgtcacgtt
     3181 cgagatgatc gacacacaac acatatgcgg tacatgcgcc gcattacggg agatcggttc
     3241 atgatgaagt gctcacctat actactctgc accgcgctga ccaccacact gttcttgacc
     3301 ggcggatgca aaaaagaggc gaccggacaa ccggccgcga acatggacgc ggcagttgcg
     3361 gccgccgcgc aaacgcctga aacctccacg gatccaaacg aggtggtcgc ttcagtcaat
     3421 gcgacccgct atctgcgcaa agacatggac cagaccgtct ctgccctcct gaaggcgcag
     3481 aacatccccg aggcccaact ggaggatgcg cgcaaacatt tcgagcaacg cgtcgtctac
     3541 gggttcatca tgaaaaccct gctgctggat gaggccaaaa agcaaacggt cacggtgaca
     3601 gaggaagatc gtaaagcgca gatcgacaag atcgccgagg cgctcaaacc ccagaataaa
     3661 acgacggaac agtatttcaa agactccccg ctgggggaaa aggttgcccg cgccgaattc
     3721 gaggacggac ttgtcatcga caagctgctg aggaaaaccg ttctggacag tatcgtcatc
     3781 gacgaggaag aggtgaagaa aaccctcgcg gagatcgaag cgagaaatgc cgaaatcgcg
     3841 gcgaccaaca aaaacctcat cgccatcaat gccgaacgca aagccaaaat tcaggaactc
     3901 aaaaaacagt tggacaacgg tgcggacttt gcggaactcg ccaaagccca ttccgagtgc
     3961 ccgagtaagg agcagggcgg cgatttgggt cccttcaccg caggacagat ggtcaagccg
     4021 tttgaagatg ccgcattcgc gcaggaaatc aacaaagtcg gggatatcgt ggagacctcg
     4081 ttcgggtacc acctgatcaa ggtgacagcc aaatcgccgg ccacggaggc caaaggcgac
     4141 acacccgcca aaccggcttc ggtgaccgca tcccacattc tgatcaagac ggaaagcccg
     4201 caagagctgc aagaagttcc cacgtccgaa cagatccaga accatctgaa acagcagaaa
     4261 tcacgcgcag cagtggaaaa atacatcgaa ggcctcaaag ccgcggccaa aatcgagacg
     4321 atttacaaag aaattccgct gtaagcgaaa cccgcgcttg cggcgtgcgg cccgaataat
     4381 cgggccgcac gctttttttg cctcccgccc acgccatcca tgcgccttta cccaaaggcg
     4441 aaacggaaaa aagatcgcgc ggcttgattc cctgtctgaa tctgataaga tattttctta
     4501 tgtctgtaaa ggttctgatg cttgacttgg tgtcagaatg gggcggtgcc caacacagcc
     4561 tctatgacgc ctgtgtttcg ctctcccgtt tgggcgttga gcctgttgcc gccgtcccgc
     4621 aagggccgtt gtttgaccgt ctgaccactg cgggcatcac cgtgttcccg gtctcgcccc
     4681 tgcgggcgca ccgtcggggc ttcgcgctgt tcaccacagc agccaaactg ctgcgcgcgc
     4741 ccgcaacggt ttcccagata ctcaaagtcg tcaaacccga catcatccat tgcaacagtt
     4801 tgcaggcctt tctcgcggtc cgcggccatc acgcaaagac cccgctcatc tggcatgtcc
     4861 gcgacctgaa gatgccggtc gccctggcgc gggacgcggc aaaaaaagcc gcgcgccttt
     4921 ttgccgtgtc cgaggcggtg gacgaatacc ttgttgagat cctgcctcaa aagcaccttg
     4981 gccgtatccg cgtggtgcgc aacgggatcg atctccagcg tttcggccag acctgccggg
     5041 tcgaggcccg cagccgtttc ggacttccgc cggatgcgcc gatagtcggc atgatcgcgc
     5101 atctcgcccc ctggaaacgc catgacgcgt tcatccggac ggccgcgcgt atccgcgcgc
     5161 gacaaccgga cgcgcatttc gtttcggtgg gtcgggatct gttcggggaa aatacccgct
     5221 ggcgcactca attgaatgcg gaggttgaga aagagcgtct ttccgaatgt tttcactggg
     5281 tcacagacct cgatcaggct gaaacgattc tgccagcgtt tgacgttctg gttcatcccg
     5341 cgttgcgcga acccttcggc cgcgtgatct gtgaggccat ggccagcggc gtcccggtcg
     5401 tcgccgccga atccggaggg ccggccaccc tgatccggca gcaggtcacc ggtgtgctgg
     5461 tccgggacgg ggatgaggag ggcatggcgg atgcggcgct cgcgctactg gcggatcgcg
     5521 tgcgcgccag ccaaatcgcg gacgcggcgc gcgcattcgt gcaggaacat cacgcgatcg
     5581 aacagacagc ggaacaactc ctcaaagagt accattcgct actggcctcc gaaaccggca
     5641 ggtacgagac ctgatgaaac cgctcacccc gtcgtttgtc tgcgccgcct aaattcatgc
     5701 aggccgacgt aggcacacag agccacggca ctcgccagca tgacattcag gataaactcc
     5761 aaacggccgg cctggagtga agccaatcca tccacccatg acgtgggcag aatcccgtaa
     5821 aaaaaggaga ctccggtcaa cccgtataac accgcgcact cgatgcccgc gatcagcgag
     5881 aacccgcaga caaaaccgaa ccggccgcac gccatctcat ggctcgtgaa acaactggcg
     5941 gcggtacgcg ccacaacgac cagcgtcaac accaccatcg cgtggtcgta acccgcataa
     6001 tcgctccaaa ccggattcag ccgcagcaac cattccccga acagggcgaa cagtcccgtc
     6061 agaatcaacc cggacacaag cgtacccccg atcgcatgcc aaagatagcg gtcggagcgt
     6121 ttcccccgct catggcgttc ggcagccagc gggaacagga caaacatgaa cgtcagcccg
     6181 acatacgccc cgatctccgc aaaacgggaa atgacgtaat accccgcaga atccagttcg
     6241 gacaaccggt ggcggatcac aaaagactcc accgcggtct gcacggcatt cgcgactgtc
     6301 atcatcgcaa ccgggagggt gtaccggagt atcgagtgcc agtctttctg ccagtacggt
     6361 tcggaacgca cctccctgcc caagtgcctc cgcagaccga acagcgaggc cccgatcaga
     6421 aacagcgagg gcgccgactg cccgacaaag taccccgaaa gggcgcggaa cggcatggcg
     6481 atccacatgg ccagcaagcg aatcggagcc gcgatcaggt tgatgaccgc caccatccgg
     6541 aactgctgca gcgcctgaag cgcatttatg aataagggcg acagcacgct gagaaccccg
     6601 gaagccacaa tcagcaggcc cagggatccc gccccgacac gcatgcgctc aaacacgagg
     6661 ggcaggatga accgggcaaa acacaacgtc ccgacaaaca gcagcaacga caggacgaac
     6721 acatcccgca acagccgctt cacctttccg tattccccgc gtgtcgcata ggtgttcagg
     6781 aacttcgtga acggcaccag cagaatcgcc agcggcaaac ccaacacgcc tcccacctgc
     6841 gtcagcggca gaaccgcccc caactcatcc tgaggcacat accggggaac cagccataag
     6901 ccgatgaaag cgttgcataa atcacacgcc ctcaaaacca gaaacagaat cgccgtgtac
     6961 caccacaacc gacccagtct cgctttgatt ttcctggcta acgcgatcat attccccgta
     7021 tccgctccac cctacgcaac cggtaagaac gcccgcttca ggaaggtcgt ctccccccct
     7081 cccgcctcgg agttcctgcg atgcgcctgt tttatatcac aagtgcggca gacccggaac
     7141 agaaaaccgc tccgagcgtg atcaagacgc gctcctgttc ccctgggcgg tgaggtttcg
     7201 tttcatgctg cggacgcctc aaggaggcgc ctcagtgccc caccacgacc tgccgccgac
     7261 atgttgcctt ttccggcttg cctcaaagcg tgaatcgggt tacgctctcc ctcggtcacg
     7321 cgttcgtaag acacttttgt ctccttcctg ctttttcaca tgaaggaagt gtacttcaac
     7381 gcgtggccgc tttcaaaact ttaggtgttg cgctggccgg tcgcgcgcgc gaggggtaaa
     7441 aatgaaaaga aggatatttt taagcgctgc ggctttagct gtggcaggcc ttgcctgcat
     7501 caccatgacg ttttgcgccc atgcggccga taccccatcg gctctggaaa aaaacgggag
     7561 ggcaaagccg caagtcttcc atcttgatct ggctttcgca aaataccgcc cggattattt
     7621 gaggcgtctg atcccgcaac tcaaaaaatg cgggttcaat acgatccttt gggagatgga
     7681 agacgctgtg cgcttcgaca cctgccccga gatcgccgcg cctgacgcca tgtcaaaagc
     7741 ggaactggcg gcgattctgg ccttcgcccg cgagtcgggg cttgaaaaca tgccgctgct
     7801 gcagacgctt tcgcataacg cctatgtcct gcgtcatccg aaatatcagc atctgtcgga
     7861 aaagccgggg ttcctcgagc tgtactgcct ttcacgtccg gagacaggac gtttcctgac
     7921 agactgggca agggaaatcg cagacctgtt cgggaacccc cgctattttc acatcggtgc
     7981 cgacgaggcg cacaaaatcg gcaaggagtg tgacctgtgc cgcaagagcg tggagaaact
     8041 gggggtcgcg ggcctcatcg tgaagcatct ctcgcccgta aagcaggcgc tctttgaacg
     8101 ccggatcacg cctgtgatgt gggcagacat ggtccacaac cacccggaac tgaaaaccat
     8161 gcttgatcgg cgcaccattc tcgccgactg gtggtatgag tctgccgagc gggccttccg
     8221 acaggtcggc ccggaaaaga agcgggtcaa tgtgttcgcc gaggcggccg aattgcggaa
     8281 atcgggtttc gccgtgtggg ggtgcgctgc ctcgcgctct tggggcgact cggtcttcgc
     8341 ccccagacac gaccaccatc tcaagaacta ccgcgaatgg cggcgggcgt tcgcggagat
     8401 ccccttggag ggcgtgatgc tgacaagctg gagctggcac ctcttcccgc tggagctgca
     8461 gatgccggtg gtcgcactgg cggcgtatga cggcttggac gacgcgcaat atgaggaagc
     8521 tttcgcacgg gattggctgg ggatcgacag caaggagttt ttccgcgccg cgcgcctttt
     8581 gtcaacaccc gtacttttca gcatgaccgc aaaagtcggc cacaaccagg tcaatcacgt
     8641 ggcggcgcct gacgcggtca tgaaggaggc gttgaagctg tcaccgaaag agaaagaccg
     8701 tgcgcgcgaa agcgaaaaag cggcggctct gctggccggg tatcaggatg ccgccagcgc
     8761 gttgcgcaaa ctggaggcgc aggcgggcag cgacaaggag atgttgcggg ac
//
LOCUS       DAOSLE010000116         8693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7600_length_8693_cov_8.375087, whole genome shotgun sequence.
ACCESSION   DAOSLE010000116 DAOSLE010000000
VERSION     DAOSLE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7600_length_8693_cov_8.375087"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            692..1588
                     /locus_tag="PK576_08125"
     CDS             692..1588
                     /locus_tag="PK576_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61597.1"
                     /translation="MKKRLIVLVFFTACVSTALAADATVKTNRLPVGAAAGGFTKCII
                     NEKPTSEDISPDRGTTAAKWFSGQWYSKMVPLDYYSTTNGVLCIEARGPGESSGDLVS
                     CPRKWDDVKNAKLPFLPGKDGFYVEFEVSLSDNNPDHFAALWLMPIEKILGKMPIYEG
                     DPEGMERWMELDVDEAGFGPGLTGTVHNWWGFKYPNYHHLQNPNNVRREKLDRTAHNV
                     FGASYDPKTRTVIWWLNGKMQMSATTPYVPSIAEKQNFYLIMGAQRRSKKIPYKMYVH
                     AVRAFTTPSSEIPAVAYDGERP"
     gene            complement(1731..4178)
                     /locus_tag="PK576_08130"
     CDS             complement(1731..4178)
                     /locus_tag="PK576_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61598.1"
                     /translation="MRVARITGTIGLLAGVSWGAADGTLMKRVLDGQDPSLQPLAMEQ
                     AAAYEQGFTRDGDEWVCDNGDDAAARRGVCWSVVLNQTRAAPVMASAEAKAEQVDGPG
                     NNDFSLYLDLSYMDGTTLWGQAMPFNPDPAVGWQRREVVLTPEKPIRRISYYLLFRNR
                     AGRVRFRDARIGVLRTEEVMRFDGVAIKAIEPPQRGFLLRDVARESGFVAIGREALGV
                     RLFTHERRAAGVTFYEVRLEEQTGRDRALTLLYSLPQPDGSLTWFHHPRRAFTLDRTQ
                     AEALNTASAPLGANGRLSRYPFGAVAVAGKGCAVGLDPATPLFFRIGCQPQTRELYLA
                     CDIGFAPEKRTADFRFCVFDFKAADGFRGALARYYELYPDAFVTRIRKQGIWMPFAPI
                     SQVEGWQDFGFRFKEGIDETAWDDAHDILTFRYTEPMTWWMTLKGEGPRTLARGREEA
                     KRLAATGSSAARAWESSAFEDERGLRPGRVLDTPWCNGVVWSINSAPGVPGAVTDFSN
                     KWNAAYLERTYGASRKADCDGEYIDSAEAYVTEVMNFRRGHFAAAERPLCYALGSRRV
                     GVFKGMIGFEYVRALEREMRARGRYMMANSTPINWFWLAPLLDVMGAETDWNRNGVWR
                     PMTDEELLYRRALCRGKPYCFLMNTDFSRFSYETSERFMKRALAYGMFPGYFSANAST
                     GHYFKRPELYNRDRPLFKKYVPLCRLVAEAGWEPVTGAVSDTPDVIVERFGWQPGLCY
                     LTVYNLTEAPRKAVIALTTLAQPTVCRELVRGGEVVWSEKRASFDLEADDVRVLEFKG
                     PYTGDVLNADSAVGRDE"
     gene            4883..6499
                     /locus_tag="PK576_08135"
     CDS             4883..6499
                     /locus_tag="PK576_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HOE61599.1"
                     /translation="MSALLLLSAVCGVHAAPAFRADRNYPDLGLRFRVLANAQPEPLA
                     QAKTYSYTFTRGSESFQRDLFDPTELWLATQHAGQWRDADGNVLVLGRPTHLFPVIES
                     KTKHVSREMFDQAMDDPAVAFAPDNPEALTAWVARFADCTPRAPERLRTGFNLADALF
                     FPVSESSRLVYAFRVKTRGKDGAGLPSDWFCAVIRIGDSTPKAKVRQAFETQFLANVT
                     WLPQSSAAVTRDVRPQTLTPVPASGPPGTPAVIPEHPSREAVRKSIADMKDWWLAETP
                     EYLFLSDIRSSAGKVLVQELQKTLPALRAGFARLVPPFKAVDEVSVLRIYESREAYQQ
                     YVGKAYAWSTGLWSPMRRELVIQWQGRNRDKTLTIIRHEAFHQYLFYASAMIEHAAWF
                     NEGHACFFEMATVDAKNRVSIPENPRVDYLMRNLDKVTRHLPKLLQTGHKEFYQGSNE
                     QRDLNYTAAWALVYYLRKGAPAEKESAYAALPDAYLQALAQTRKAEAATAAAFEGVNM
                     DLFQRRFTEFWQRGRNRGQRYDPFGKLTAD"
     gene            6520..7422
                     /locus_tag="PK576_08140"
     CDS             6520..7422
                     /locus_tag="PK576_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE61600.1"
                     /translation="MPRLAPPPQGDYNTIDRMNIPCPVFDFHAHLYPDALARNVVEPL
                     ARRFGNPAAFDGTVDGMTRELAASGLCGALNLPVATKPGQVNSINTWAAANNRGPVYS
                     LATLHPDTPDIPDTLGALLAAGFRGVKLHPEYQTFRLDDLRLEPVWETCAALGLFVFL
                     HAGGERVFAPPFHSTPTDVAALLDRHPKLTLVAAHLGGFQMWDEAEERLIGRPLYLDL
                     SHTFFWMPDGQILRMVRRHGADRILFGSDAPWQKPAAVLAAFLNLPLREAEQRMILWE
                     NAARLLQLPVAERLAVPPPNPQTR"
     gene            7430..8269
                     /locus_tag="PK576_08145"
     CDS             7430..8269
                     /locus_tag="PK576_08145"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006307.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOE61601.1"
                     /translation="MTIAIIGLGLIGGSFARDLRKAGLGRTLLGVETSPEHAERALEL
                     GLVDRVVPLSEAVATAQVLLLATPVDAIERLLPQLLDAVESHQTVIDLGSTKFRIGEG
                     VREHPKRERYVAAHPMAGTENSGPDAALEDLFRGKTVLLCNLEWSAPDACKTALEIFH
                     AVGLKFEFMNAEQHDRHAAYVSHLSHAVAYALSLAVQSEEKAGYAVPRLAGGGFTSTV
                     RLAKSSPEMWVPIFEQNREAILKAVEAFSERMLTFKQCLEERDYVRLDALIREANQIR
                     DLL"
     gene            8360..>8693
                     /locus_tag="PK576_08150"
     CDS             8360..>8693
                     /locus_tag="PK576_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOE61602.1"
                     /translation="MNKIIRKTQLSEEIFRMEVEAPLIARERKPGQFIILQVDDDFGE
                     RIPLTIADADPERGSITLVFQTVGKTTHLLAEKEEGDTIVALLGPLGQPSRIEKVGHV
                     VCVGGGIGV"
BASE COUNT         1718 a         2771 c         2516 g         1688 t
ORIGIN      
        1 gcaacggagg tgccggttca ggcgcgcgct ccttcacgaa cgcagcctcg aacgcggcac
       61 gttccgtcgc gccgccaagt gcacctacgg ccaacccaga taagaaaacc ccgacagcaa
      121 tgtgtttaat gttcatcttt ccggctcccg taatggtggc cactccacgt ggccaccatt
      181 acgtcaacag gcgcgtcgtt catcaatgcg tcgtcgttca taccagcttg ctcaaacagt
      241 ccagacttct ggacgcctga tccggcgtgc cgtagggtca actgagaacc gtaacgttca
      301 ccatatcgtt aaggagtatg acataattcc ggagagatcg cgattgtatt ttttcaccaa
      361 ttgattgcga gaacacttag gcttcgcgca catgttccag ccataggacg ccgccggttt
      421 tggcgtccgt gtaacggtgc ttgaaccggt cgccctgcca acgtgcgccc gcccgataat
      481 ccgggcgccc cgttgttgcc gtttccggct tgccgcaaca tgtaactctg gctatgcttg
      541 cgatcgttcc tgtgcgtgtg tgacgtgttt tctccgttcg tgatcatcac acgaggagtg
      601 tgcacttcac caatggccgt tttcaaaact tcacgtgcta ccctgaccgg tcgcatgtgc
      661 ggaggtcttt gaccggaaag aagcgatgag gatgaaaaag cgtctgatcg tattggtgtt
      721 ttttacagct tgcgtgtcca cggctctggc ggcggatgcg acagtgaaaa ccaaccggct
      781 gcctgtgggg gccgcagcgg gcggtttcac gaagtgcatc atcaacgaga aaccgacatc
      841 cgaagacatt tctccggatc gcggcaccac ggccgcaaag tggttcagcg gtcaatggta
      901 cagcaaaatg gtgccgcttg attactattc gacgacgaac ggtgtcctgt gcatcgaagc
      961 tcgcggtccc ggagaaagca gcggtgacct cgtatcgtgt ccgcgcaaat gggacgacgt
     1021 gaaaaacgcg aagctcccgt ttcttccagg taaagacggg ttttacgtgg aattcgaggt
     1081 gagtctttcg gacaacaacc ccgatcattt cgctgccttg tggttgatgc cgattgaaaa
     1141 gatcttgggc aaaatgccga tttatgaagg cgatcccgaa ggtatggaac gctggatgga
     1201 gcttgatgtg gatgaagccg gtttcgggcc cggtttgacg ggcacagttc acaactggtg
     1261 gggattcaaa tacccgaatt accatcacct gcagaacccg aacaatgtgc ggcgcgagaa
     1321 actcgaccgt accgcacaca acgtctttgg cgcgagttac gatccgaaga cacgaacggt
     1381 tatctggtgg ctgaacggga agatgcagat gagcgccaca accccgtacg tcccctcgat
     1441 cgccgagaaa cagaacttct atctcatcat gggggcacaa cggcgcagca agaagattcc
     1501 ctataagatg tatgtccacg cggtacgcgc gttcacgaca ccctcgagtg aaattccggc
     1561 tgttgcttac gacggcgagc gtccttgacc cttggaaatt ctctccagcc tcaccgtgcg
     1621 cggcgacggt actggccggg tgtttgaccg cacttacgcc tgaaaatgga cgaaagatat
     1681 tgcgcatggc agaaccccgt gcgacgcgcg cattttgtcc ggctgacgca tcactcgtcc
     1741 cggccgaccg cactatcggc gttcagcaca tcccccgtat acggcccctt gaactccagc
     1801 acgcgcacat cgtccgcctc cagatcaaag ctcgcgcgct tttcgctcca gaccacctcc
     1861 cctccccgca ccaactcccg acacacggtc ggttgcgcga gcgtggttag cgcgatgacc
     1921 gccttccgtg gcgcctccgt aagattgtag acggtcagat aacagagccc cggctgccag
     1981 ccgaaacgct ccacgatgac gtcgggcgtg tccgataccg cgccggtcac gggctcccaa
     2041 cccgcttcgg cgaccagacg gcagagcgga acatattttt tgaataacgg acggtcacgg
     2101 ttgtacaact caggacgttt gaaatagtgg ccggttgagg cgttggcgct gaaatagccg
     2161 ggaaacatgc cgtaggccag ggcacgtttc atgaatcgct ccgaggtctc gtatgagaat
     2221 cgggagaagt cggtgttcat caggaagcag taaggcttgc ccctgcacag cgcgcggcgg
     2281 tagagtaatt cctcgtcggt catcggccgc cagacgccat tgcggttcca atcggtctcg
     2341 gcacccatca catccagcaa cggggccagc caaaaccagt tgatcggcgt gctgttggcc
     2401 atcatgtagc ggccgcgcgc gcgcatctcc cgttccagtg cccgcacata ttcgaatccg
     2461 atcatcccct tgaacactcc cacacgacgt gagccgagcg catagcagag cggacgttcc
     2521 gcagccgcga aatgcccgcg ccggaaattc atcacctcgg tgacgtatgc ctcggccgaa
     2581 tcgatgtact cgccgtcgca atccgcctta cgcgaggcac cgtacgtcct ttcgagatag
     2641 gcggcgttcc atttattcga gaaatccgtg accgcgcccg gcacgcccgg cgcggagttg
     2701 atgctccaca ccacgccgtt acaccagggc gtgtccagca cccgaccggg cctcaggcca
     2761 cgctcatcct cgaaagcact gctctcccac gcccgcgcgg ccgagctgcc cgtcgcagcc
     2821 agccgcttgg cctcttccct gccgcgcgcg agggtgcgcg gcccctcgcc cttcagggtc
     2881 atccaccagg tcatcggttc cgtgtaccgg aaagtgagaa tgtcgtgcgc gtcgtcccaa
     2941 gcggtttcat caatcccctc cttgaaacgg aagccgaagt cctgccaccc ctccacttga
     3001 ctgatgggcg cgaacggcat ccagattccc tgtttgcgga tacgcgtcac aaaggcgtca
     3061 ggatagagtt cataataccg cgccaacgca ccgcggaacc cgtccgccgc cttgaaatcg
     3121 aaaacgcaga aacggaagtc cgccgtgcgt ttctccggag cgaacccgat gtcgcacgcc
     3181 agatacagct cgcgggtctg cggctgacaa ccgatccgga aaaacagcgg cgtcgcagga
     3241 tcgagtccga cggcacagcc tttgcccgct acggcaaccg caccgaaggg atagcgtgaa
     3301 agcctcccgt tcgcgcccag aggcgcgctg gcggtattca gggcctccgc ctgcgtacgg
     3361 tccaacgtga aggcgcggcg cggatggtga aaccacgtca gactgccgtc cggttgcggc
     3421 agggagtaga gaagcgtcag cgcacgatcc cgcccggtct gctcttcgag gcgcacctcg
     3481 taaaacgtca cgcccgcagc acggcgctcg tgcgtgaaga gccgtacacc gagcgcctcg
     3541 cgcccgatcg cgacgaaccc cgactcccgc gccacatcgc gcagcaagaa accgcgctgc
     3601 ggaggctcga tggccttgat cgccacaccg tcgaaccgca tcacctcttc ggtgcgcagc
     3661 accccgatgc gcgcgtcgcg aaagcgcacg cggcccgcgc ggttacggaa caacaggtaa
     3721 tagctgatcc gacggatcgg tttttcggga gtgagcacca cctcacgccg ctgccaacct
     3781 acggcggggt cgggattaaa cggcatggcc tgcccccaaa gcgttgtgcc gtccatatag
     3841 ctcagatcca gataaaggga aaaatcgttg ttgcccggac catcgacctg ttccgccttg
     3901 gcttcagccg aggccatcac cggcgcggcg cgggtttgat tcagcaccac gctccagcag
     3961 acaccgcgcc gtgccgcggc atcgtcgccg ttatcgcaca cccattcgtc gccatcccgc
     4021 gtgaacccct gttcatatgc ggccgcctgc tccatagcca aaggttgcag cgacggatcc
     4081 tgcccgtcga gaacacgctt catcagcgtt ccgtccgccg caccccacga caccccggcc
     4141 agcaggccga tcgtccccgt tattctggca acacgcatac acaccccttt gtcaggcgga
     4201 accggggaaa acccgcttca tacggaaccg cacccccgga accgcttctc cacaaaccat
     4261 acagacagcg tacccttgtc gcgtctgttt gtccaaccga aagacgtctg gaaacgggca
     4321 tcgggcgcga gttcgtgcct tacggcttac gaatccggcc acaaacagca ttgcacgttg
     4381 aacaccaaga tacccctgct ttcttgggtt tcaaggtttc ggagtttcag ggtttcaggg
     4441 tttcagggtt tcatggtttc tgggtttctg gtttcagggt ttcagggttt catctaaatc
     4501 ggggtcggga tcgctatcgc tatcgggatc ggaatcggga tcgctatcgg gatcgattgc
     4561 ttaacctaaa aagagcagtt gaactttcaa ctctctttgg tttctgggtt tcatggtttc
     4621 tgggtttcag ggtttcgtgg tttcatggtt tcatccaaat cggggtcggg atcgctatcg
     4681 ggatcgattg tttcaccacg ggacacaaga acacacggaa agtcatggct tggtttcatg
     4741 ccacgcgtgc ggcataaatt gtggccgggg tagtatctgc accggccgcg tcttgccgga
     4801 tttccgggca cgcgctatac tcccccgcat gcgtatttct cgaagtcccg ttgcgatcgc
     4861 gacgtgccgg aatgtgcggt acgtgtccgc gttgctgttg ttgtcggccg tgtgcggggt
     4921 ccatgccgcg cctgcgttcc gtgccgaccg gaattacccg gatctcggat tgcgtttccg
     4981 tgtgctcgcc aatgcgcagc ccgaaccgct cgcgcaggcc aagacctatt cgtatacctt
     5041 cacccgcggc agcgagtcat tccagcgtga cctgttcgac cctacggaac tctggctcgc
     5101 gacccagcat gcgggccagt ggcgcgatgc ggacggcaat gttctggtgc tcggtcggcc
     5161 gactcacctg ttccccgtga tcgagtcaaa gacaaaacac gtctcccgcg agatgttcga
     5221 ccaggccatg gacgatcccg ccgttgcctt cgccccggat aatccggagg cgctcaccgc
     5281 atgggtcgcg cgctttgccg actgcacgcc gcgtgccccg gagcgactgc gcaccgggtt
     5341 caacctcgcc gacgccctct tcttcccggt cagcgaatcg tcgcgtctgg tgtacgcctt
     5401 tcgcgttaag acacgcggca aggacggcgc gggcttgcct tcagactggt tctgcgcggt
     5461 catccgcatc ggcgattcca caccgaaagc caaagtgcgg caggccttcg agacccagtt
     5521 cctagccaac gtgacgtggc tcccgcaaag cagcgccgcc gtgacaaggg acgtccggcc
     5581 ccaaacgctc acgcctgtcc ccgcctccgg ccccccgggc acccctgcgg tcattccaga
     5641 gcatccgagc cgcgaggccg tgcgtaaaag tatcgccgat atgaaggact ggtggcttgc
     5701 cgaaaccccg gagtacctct tcctctccga tatccgcagt tcggcgggca aagtgctggt
     5761 gcaagaactg cagaagaccc tcccggccct gcgcgccggt ttcgctcggc tggtaccgcc
     5821 cttcaaggcc gtagacgagg tgagtgtgct acgtatctac gaatcgcgcg aggcttatca
     5881 gcagtatgtg ggcaaagcgt atgcgtggag cacgggactc tggtcaccca tgcggcgcga
     5941 actggtgatt cagtggcaag gccggaatcg ggacaagacc ctcaccatca tccggcacga
     6001 ggcgtttcac cagtatctgt tttatgccag cgccatgatc gagcatgccg cctggttcaa
     6061 cgaaggccac gcctgtttct tcgaaatggc gacggtcgac gccaagaacc gggtctccat
     6121 cccagagaat ccgcgtgtgg actacctgat gcggaatctg gacaaggtga cgcgtcacct
     6181 acccaaattg ctccaaaccg gtcacaagga gttttatcag ggatcgaacg agcagcgcga
     6241 cctgaactac accgcagctt gggcacttgt gtactacctg cgcaaaggcg caccagcgga
     6301 gaaggaaagc gcgtatgcgg ccctgcctga cgcgtatctg caagccctcg cgcagacccg
     6361 gaaggcggag gctgcgaccg ccgccgcctt cgagggggtc aacatggacc tcttccagcg
     6421 ccgtttcacg gaattctggc aacgcggacg caaccgcggt caacgctatg atccgttcgg
     6481 aaaactaacc gcggactgag ccgcggcccc gtcgagggag tgccgaggct tgcgccgccg
     6541 ccgcagggtg attacaatac catcgaccga atgaacatcc cctgtccagt tttcgatttc
     6601 cacgctcatc tctatcctga cgccttggcg cgtaacgttg tggagccgct cgcgcggcgt
     6661 ttcggtaacc ctgcggcctt tgacgggacc gtggacggca tgacgcgcga actggcggcc
     6721 tccgggttgt gcggcgcgct gaatctgccg gttgcgacca agcccggcca ggtgaactcc
     6781 atcaacacct gggccgccgc caacaaccgc ggcccagtct acagtctggc gaccctccat
     6841 ccagacacgc ccgatatccc tgacacactt ggggccctgc tcgcggcggg ctttcggggc
     6901 gtcaaactgc atcccgaata ccagacgttc cggctggatg acctgcggct cgaaccggtc
     6961 tgggaaacct gcgccgcgct ggggctcttt gtgttcctgc acgccggcgg cgaacgggtc
     7021 ttcgcgccac ccttccattc cacaccgacg gacgtggccg cgttgctcga ccgccatccg
     7081 aagttgactc tggtggccgc gcacctgggc ggattccaaa tgtgggacga ggcggaagag
     7141 cggttgatcg gacgaccgct ctatctggat ctttcgcata cgtttttctg gatgccggat
     7201 gggcagatcc tgcggatggt acggcggcat ggggcggacc ggatcctctt cggcagcgac
     7261 gcgccctggc aaaaacctgc ggcggtgctg gccgcttttc taaacctacc gttgcgggaa
     7321 gccgagcagc gcatgatcct ctgggagaac gcggcccggc tcctgcaact cccggtagcg
     7381 gaacgcctcg ccgttccgcc gcccaaccct caaacaagat aggcgaccca tgacgatcgc
     7441 aattatcggt ctcggtttga tcggcggctc tttcgcccgg gatctgcgta aagccggtct
     7501 cggtcggacc ctgctcggtg ttgaaacctc cccggaacac gcggaacgcg cgctcgaatt
     7561 gggactggtc gaccgggtgg tgccgctttc cgaagcggtg gcgaccgcac aagtgctgct
     7621 gctcgccaca ccggtcgacg ccatcgagcg gttgctgccg caacttctgg acgctgtgga
     7681 atcccatcaa accgtcatcg atctaggatc caccaagttc cgcatcggtg aaggtgtccg
     7741 ggaacacccg aaacgggaac gctacgtggc ggcgcatccg atggccggca cggagaactc
     7801 ggggccggat gccgcgctcg aagatctgtt ccgcggcaag accgtcctgc tctgcaacct
     7861 cgagtggagc gcgcccgacg cctgcaaaac cgcgctggag attttccacg ccgtggggct
     7921 gaaattcgaa ttcatgaatg cggaacagca cgaccgccac gccgcctatg tctcgcatct
     7981 ctcccacgcg gtagcgtacg ccctgtccct tgcggtgcag agtgaggaga aagccggata
     8041 cgccgtaccg cggctcgcgg gcggcggatt cacctccaca gtgcgtctcg ccaaaagttc
     8101 gccggagatg tgggttccga ttttcgagca gaaccgcgaa gccatcctca aagccgtcga
     8161 ggctttttcc gagcgcatgc tgacgttcaa acaatgcctt gaggagcggg actacgtacg
     8221 gctcgacgcg ctgatccgcg aggctaacca gatccgcgat ctgctctgac ccgatggttc
     8281 ggcggctttc ttgcggctgc ggaaaaaacg tgttatgctc gccccgtttt gtggtcctcc
     8341 tgttcgatga aagacgatta tgaataaaat catccgaaaa acacagctct ccgaagagat
     8401 cttccgcatg gaggtcgagg caccgctgat cgcgcgcgaa cgcaagcccg gccagttcat
     8461 catcctgcaa gttgacgatg atttcggcga gcgcattccg ctcacgatcg ccgacgccga
     8521 tccggaacgc ggcagcatca cattggtttt ccagacggtc ggtaaaacca cccacctgct
     8581 ggcagaaaaa gaggagggtg acacaatcgt cgcgctactg ggaccgctcg gccagccctc
     8641 ccgcatcgag aaggtcgggc atgtggtctg cgtcggcggc ggcatcggcg tgg
//
LOCUS       DAOSLE010000117         8665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7624_length_8665_cov_9.697909, whole genome shotgun sequence.
ACCESSION   DAOSLE010000117 DAOSLE010000000
VERSION     DAOSLE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_8665_cov_9.697909"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="PK576_08155"
     CDS             <1..284
                     /locus_tag="PK576_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE61603.1"
                     /translation="IVYVVTRVIGKVAGATAGAKLANMPKTVQKYLGLTLVPQAGVAI
                     GLSLAASRILGDSHGHEIRTIILAATVLYELSGPIITKIALTKAGEIEA"
     gene            complement(562..2922)
                     /locus_tag="PK576_08160"
     CDS             complement(562..2922)
                     /locus_tag="PK576_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61604.1"
                     /translation="MKRTSPSTIILALLIVATSSLCAREHDLAAIIKTQAERSSVWTR
                     VQDLTPTMTSRELFAYALLLCEAQLHLDRLDRLFEVAARMQDRNPESRGYGNFRWSWR
                     DGAVFDFNAGDFCMENAAVIWIKHRKLLTDKQRKDLSELIDHAIAGCLRHRVSSGYTN
                     IAILNAQNLILMGEMLGRDDVLAEGRKRLDDFCAYTAVNGIREYCSPTYAGVDLICLH
                     RLHTYCVTPAVRGQCEALLNVFWLDAIANCFAPSFRLGGAHSRDYDYLGGRGGVDAWL
                     AAAGCFASPNPSAAPPLLWLQSTWRPPTAWLRDLQVAPRVVTQVWGPGGSEYRVMWAG
                     RHVALSIAGKTYHNMDAPMVVNFAGPHTMPRGYFIADGRRDPYGKKVIPEGNGPHRKT
                     LHLMPHWYAVQDGQDALGLALHRREDIRDCPTLESHFVLPYPCDELFIGNHTVVFDAK
                     APKTVPVSSGAAVVIRHGAAAAAIRVIWSQNTVREPAPVALIHDGNPHGVIRLTVAHH
                     DQWGMPINTDAPVGAAFWIRVCDLADNAVHFARWRQEFAAATADARCDNASLAITAGS
                     TSGQTLRLLTHKPFRALSDAEPVPARAVLAVNGRDLGGEHLARLPVVVAYKRQIAEAL
                     TRQREGAVMILPDRDAIWEAEAGAVFPNMVVAKDDPQASGGSYVWAPGEPGARGGGTG
                     YLLQNLQVAKAGQYYLSARVIAPSFEDDSFFISVSSEKFSILDQFVWHLPKVNAEWYW
                     AMVADRGNSDGIPLQLPEGTVTLRIQVREDGTKIDQLRLSPQPGVR"
     gene            3135..4640
                     /gene="zwf"
                     /locus_tag="PK576_08165"
     CDS             3135..4640
                     /gene="zwf"
                     /locus_tag="PK576_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOE61605.1"
                     /translation="MPKKRSAGTFCIEVAPSPCVLVLFGATGDLARRKLFPSLFQLHR
                     LGLLHAATRIVACGRSPVTDEAFREQSAAFLPQADPQTRTAFMSRLTYRQADAAEPST
                     FRDLATYLDGLDHAPESPVPLNRLYYLAVPPSSYRALITLLDNTGQINEHDHDQGPWR
                     HLVLEKPFGSDTATAADLDAFLKTVAKEEQLFRIDHYLGKETIQNIMVTRFANIIFDP
                     IWDARYVDHVQITAAETLGVEGRAAYFDHTGLLRDMFQNHIIEMLALTAMEPPERFDA
                     DAIHDSKLKLIRAIRPFDTGSDANAIVRAQYSAGRGMPAYREEAGIPTQSETETFVAA
                     KLWIDTPRWAGTPFYLRSGKRLAANSNAITLVFKQAPHLDLGGPGANALILRSRPGEG
                     MCLHLLGKHPGPKLCVGTLPLSFDYAELAPGNEAPDAYARLLLDAMLHDHTLFVRSET
                     IRTAWELFTPVLDLWRDCPDRMPLHTYEAGSAGPAAAADLMAADGREWSSL"
     gene            complement(4855..6753)
                     /locus_tag="PK576_08170"
     CDS             complement(4855..6753)
                     /locus_tag="PK576_08170"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE61606.1"
                     /translation="MDTDRTENQAPRIFELPVHTFHTRLLRLLVRIVKRPVESITGLR
                     ACDKIYARTWRMPKDIPFCDRALQAIGIRTDFSEADLARIPKEGAVVVVANHPFGGIE
                     GIILLSLLRRVRPDVKAMANYLLGAIPEMQGDFILVDPFGSKNSAKANLRPLKESLSW
                     LKEGHLLIVFPSGEVSSFDRRTLRVRDPAWSASIAALTRKTNATVVPMFFPGRNNVFF
                     QIAGFLHPRFRTSLLPRQIANKTGWKITVRIGTPLSPKALEPFSLDDVQLIRYMRFRS
                     YLLAERETHRTRRFISLNPPMLPADPIIAPVPCETLTREIASLPESCRLIAAGDFAVY
                     IAAAHQIPSCLREIGRLRETTFRVVGEGTGNAIDLDAFDEYYNHLFMWNTARQEIVGS
                     YRLGLTDRILTERGIKGLYTRTLFKFDERLMTRIPPAIEMGRSFIRTEYQKAYTSLFL
                     LWKGISAFIGRNPHYRVLFGPVSITNEYREASRCMILASMRQTCMADELTWLVKPKFP
                     PKPPRRSEWCLKEYMEYLNDIDRVADYVAEIEPDGKDIPVLLRQYLKLSGKLLAFNVD
                     PDFSSVVDGLIMVDLSQSSPKTLRRYMGNEVAEAYFRYHGITESTDDPAGKREPHSTP
                     AASSPTPP"
     gene            complement(6743..8056)
                     /locus_tag="PK576_08175"
     CDS             complement(6743..8056)
                     /locus_tag="PK576_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE61607.1"
                     /translation="MTRAYIRPLSRAAFPGFALAALCAAPTQWSLIPSLRIGVADLLL
                     ACAAALVVLECLLARDGKKALSLRPHWTHLLFILASGAALSAATDRMDALKEWVQGVE
                     YFIIAPVVFTALLRKERLFSNASAEGAASPAASRVPPGFIALGAVTAVVLGLAVIQYL
                     APEGHDSIRLFAEPKTLDIIVNVLGGRESPLLVGGTFGNRNVLGGFLALALPLCAVGA
                     LASGRAIWTRIACALLLLTGLAVNLSGASYWAVVVVIAAIAATRGARVFLPVMLALML
                     WQTEVLKNLPRENDLCHFESVALYDSAGLPTRRYPEWQAAVGMILTHPWLGVGPGHYQ
                     KRIGSFYGQVPNATGPAEPDIQNLYLVLGASCGLPALFAFLLMLASALGAATRSGRWG
                     AAGALAAFAVTAVWHPLLVRGIGLPLAFVLALAHHPKSPEADYGY"
     gene            complement(8053..8580)
                     /locus_tag="PK576_08180"
     CDS             complement(8053..8580)
                     /locus_tag="PK576_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61608.1"
                     /translation="MQRMILLAGLFLICRAQAKGVRICWEAETANRIEPPVTVVSNGI
                     EGASGAYLEIAEGAGNPPKVNAGVAGFDLDIPEDGVFTLWCRVWWEGECSNSFTVRVD
                     DQPPFLFGEDATYKTWHWVKYPVSRTAPPLRLTRGRHTLTFLNREDGVRLDQILLSSD
                     RRFVPTDIEKTGVRQ"
BASE COUNT         1711 a         2564 c         2804 g         1586 t
ORIGIN      
        1 cgattgtcta tgtcgtgacc cgcgtcatcg gcaaggttgc cggggcaaca gccggggcca
       61 agctcgcgaa tatgcccaag accgtccaaa agtatctggg gcttacgctc gtgccgcagg
      121 cgggcgtggc gatcggcctg tcgcttgcgg catcacgcat cttgggggat tctcacgggc
      181 atgagatccg cacgattatt ctcgcggcga ccgttctgta cgagctgtcg gggccgatca
      241 ttaccaagat cgcgctgaca aaagccgggg agatcgaggc ctaatgatgc gggcgaagcc
      301 cgcaactcaa gttcattagg tcattagtta attagtgcat tagttaatta gtgcattagt
      361 taattagtgc attagttaat tagtggatta gtggattagt ggattagttt gtctgggagc
      421 gcgctgccgc gcttcgcagt gctcctccaa aaaaccttgg cgttcttggc gccttggcgg
      481 ttcccaaaag ccgatcaatg ccccccaaaa aaaccttcgt gcccttgtgt gagaaacaac
      541 ttttttattg gttgcaaaaa gtcagcgcac accgggttgg ggcgacagcc gcagttgatc
      601 gattttggtg ccgtcctcgc gaacctgtat gcgcagggtc accgtgcctt caggaagttg
      661 cagggggatg ccgtcggaat tgccgcggtc agcgaccatg gcccagtacc actccgcgtt
      721 caccttcggc aaatgccaga caaactggtc caggatagaa aacttctcac tgctgacgga
      781 gataaagaac gagtcgtctt caaacgaagg cgcgatgaca cgcgcggaga ggtagtactg
      841 cccggctttg gcgacttgga ggttttgcag gagatagcca gtcccgccgc cgcgcgcacc
      901 gggttcgccg ggagcccaca catagctgcc ccctgatgcc tgcgggtcgt ccttggccac
      961 caccatgttc ggaaagaccg cgccggcttc cgcttcccag atggcatcgc gatcgggcag
     1021 gatcatgacg gcgccttccc gttgtcgcgt gagtgcctcg gcgatctgcc gtttgtacgc
     1081 gacgacaacc ggtaagcggg ccaagtgttc tccgccgagg tcacggccat tgacggcaag
     1141 gacggcacgc gcgggcacag gttcagcatc actcagcgcc ctaaagggct tgtgcgtgag
     1201 caggcgtagc gtctgaccgc tggtgctgcc ggcggtgatc gcgaggctgg cgttgtcaca
     1261 acgcgcgtcg gcggttgcgg cagcgaactc ctgccgccac cgcgcaaagt gcacggcgtt
     1321 gtcagccaga tcgcagacgc gaatccagaa cgcggcgccg acgggggcgt ccgtgttaat
     1381 gggcatgccc cattgatcgt ggtgggcgac ggtgagacgg atgacaccgt gggggttgcc
     1441 gtcgtggatg agcgcgacgg gcgcgggttc gcgaaccgta ttctggctcc agatcacacg
     1501 gatcgccgca gccgccgcac cgtggcggat gacgacggct gcgccggatg acaccggcac
     1561 tgttttgggc gctttggcgt cgaagaccac ggtgtgattg ccgatgaaca gttcgtcaca
     1621 agggtaaggc aacacaaagt gggattcgag ggtcgggcag tcccggatgt cctcccgccg
     1681 gtgcaatgcc agaccaagcg catcctgacc atcctgaacc gcataccagt ggggcatgag
     1741 gtggagagtt ttccgatgcg ggccgttgcc ctccggaatc acttttttcc cgtagggatc
     1801 gcgacggccg tcagcaatga aatagccgcg tggcatggtg tgcggaccgg cgaaattcac
     1861 gaccatcggc gcatccatgt tgtggtaggt cttgccggcg atgctgagcg cgacgtgccg
     1921 gccggcccac atgacgcggt attcgctacc gccgggaccc cacacctgcg tcaccactcg
     1981 cggcgcgacc tgcagatcgc gaagccaagc cgtcggcggc cgccaagtgc tctgcagcca
     2041 cagcagcggc ggcgcggcac tcggattcgg cgacgcgaaa cagcccgccg cagccagcca
     2101 cgcatcgaca ccgccgcgac cgccgaggta gtcgtagtcg cggctatggg cgccgcccag
     2161 gcggaaggac ggcgcgaagc agttggcgat ggcatcaagc cagaagacat tcagcaacgc
     2221 ttcgcattgc ccgcgcacgg ctggcgtgac gcagtacgtg tgcaggcggt gcaggcagat
     2281 caggtcgacg ccggcatagg tcggactaca gtattcacgg atgccgttta ccgccgtgta
     2341 ggcgcagaag tcgtccagac gcttgcggcc ttcggcgagc acgtcgtcgc gtccgagcat
     2401 ctcgcccatg agaatgaggt tctgggcgtt caggatggcg atgttggtgt aaccggagga
     2461 cacgcggtga cgcagacacc ccgcgatggc gtggtcgatc aactcactca ggtccttgcg
     2521 ctgtttgtcg gtgagcagct tgcggtgttt gatccagatg actgcggcat tttccataca
     2581 gaagtctccg gcattgaaat cgaagacagc gccgtcgcgc cagctccagc ggaagttccc
     2641 gtagccacgg ctttcggggt tgcgatcctg catgcgtgcg gcgacctcaa agagccggtc
     2701 caagcggtcg aggtggagtt gcgcttcgca gagcagcagc gcataggcga agagttcacg
     2761 ggaggtcatg gtcggcgtca ggtcctgcac gcgggtccac acgctcgaac gttccgcctg
     2821 ggttttgatg atggcggcga ggtcatgctc ccgcgcacac agcgacgaag tcgcgacgat
     2881 gagtaatgcg agaataatgg tgctgggaga tgttcgtttc acgggacctc ctataatgcc
     2941 tgcgacagtg taaacatgcc ctttgagatt agggtaaagg tgtctctaag gcaaggccaa
     3001 cgatgtgcgc tttggtttta gatttctggt ttcacgtttc atccgcagat gggatttcct
     3061 tttttggggg gtcaccctag gaggaaaacg ccttgcggat tgacttcgcg ccttaattgc
     3121 gttaccaatc ggacatgcca aagaaaaggt ctgcgggaac attttgtatc gaggtcgcgc
     3181 ccagcccttg cgtgctggtg cttttcggtg ccaccggcga tcttgccaga cgcaaacttt
     3241 tcccgtcgct gttccagctg caccgccttg gcctcctgca cgcggccacc cgcattgtcg
     3301 cttgcggacg ttcccctgtg accgacgaag ccttccgcga gcaatccgcc gccttcctgc
     3361 cgcaagccga cccgcagacc cgtaccgcgt tcatgagccg tctgacctac cgacaggcgg
     3421 acgcggctga gccctccact tttcgtgatc tcgcgacgta tctggatgga ctggaccatg
     3481 cgcccgaatc gcccgtgccg ctgaaccgcc tctactatct ggccgtgccg ccttcctcct
     3541 accgggcgct catcaccctg ctcgacaata ccggccagat caacgagcac gaccacgacc
     3601 aaggcccgtg gcggcacctg gtgttggaaa agccgttcgg ctcggatacc gctactgccg
     3661 ccgatttgga cgccttcctt aaaaccgtgg caaaggaaga gcagcttttc cgcatcgacc
     3721 actacctcgg caaagagacg atccaaaaca tcatggtcac gcgctttgcc aacatcattt
     3781 tcgatccgat ctgggacgcg cgctatgttg accatgtgca gatcaccgca gcagaaaccc
     3841 tcggtgtcga gggccgcgcc gcctatttcg accacaccgg gctgctgcgc gacatgttcc
     3901 agaaccatat tatcgagatg ctggctttga cggccatgga gccgcccgaa cggttcgatg
     3961 ccgacgcgat ccacgacagc aaactgaagc tcatccgcgc catacgtcct ttcgataccg
     4021 gcagcgacgc gaacgcgatc gtgcgggccc aatatagcgc tggccgcggt atgcccgctt
     4081 accgcgaaga ggcgggcata cccacccaat cagagaccga gacctttgtg gcggcgaaac
     4141 tctggatcga cacaccccgt tgggcgggaa ccccttttta cttacgctca ggcaagcggc
     4201 tggccgcaaa cagcaacgcg atcacgctcg tgtttaaaca ggcgccccat ttggatctgg
     4261 gaggtcccgg tgccaatgcg ctcattttgc gctcacgccc cggtgagggc atgtgcctgc
     4321 atctgctggg caagcacccc ggtcccaaac tgtgcgtggg gacgctgccg ctctcgttcg
     4381 actatgccga acttgccccg ggcaacgagg ccccggacgc ctacgcccgc ctgctgctgg
     4441 acgccatgct gcacgaccac acgctgtttg tgcgcagcga aaccatccgc accgcgtggg
     4501 aacttttcac gcctgtactg gatttgtggc gtgactgtcc ggaccggatg ccgctgcaca
     4561 catatgaggc cggctcggcc gggccggccg ccgccgccga cctgatggca gcggacggcc
     4621 gcgaatggtc cagcctgtaa agctgaagca agcaaccgtg ccgctacggc tcgcaaacct
     4681 tgtgaacgga ggagtgagcg caatggcgga gcggcggaga gcgttgcctg atcaaacaga
     4741 tcggacggat caggctgatc accaaatgag agggcggcag gggagcagga aattacgccc
     4801 gagcgatgat taccgccaag acgccacaga cgccaagagg gatggaggag gcgattatgg
     4861 tggagtgggg gatgatgctg ccggtgttga gtgaggctca cgctttcctg ccgggtcgtc
     4921 ggtggattcg gtgatgccgt ggtatcggaa gtaagcttcg gcgacctcgt tccccatata
     4981 gcggcgcagg gttttcgggc tggactggga aaggtccacc atgatgaggc cgtctaccac
     5041 tgagctgaaa tccgggtcca cgttgaaggc gagcagtttg ccgctgagtt tcaggtattg
     5101 gcgcagcagc accgggatgt ctttgccgtc gggttcgatc tccgcgacgt agtccgccac
     5161 ccggtcaatg tcgttgagat actccatata ctccttgagg caccactccg aacggcgcgg
     5221 cggtttgggc gggaatttgg gcttcaccag ccaggtcagc tcatccgcca tgcaggtctg
     5281 gcgcatggaa gcgaggatca tgcagcgtga ggcctcgcgg tactcgttgg tgatgctgac
     5341 cgggccgaac agcacacggt agtgcgggtt gcgcccgatg aaggcggaga tgcctttcca
     5401 cagcaggaac agcgatgtgt aggccttctg gtattcggtg cggatgaagg agcgtcccat
     5461 ctcgatggct ggcggaatgc gcgtcatcag gcgctcgtcg aatttgaaca gtgtgcgcgt
     5521 atagaggcct ttgattccgc gttccgtgag aatgcggtcg gtcaggccga gacggtacga
     5581 gccgacgatc tcctgacgcg cggtgttcca catgaagagg tggttataat attcgtcaaa
     5641 ggcgtcgagg tcgatcgcgt tgccggtgcc ttcgccgacc acgcggaagg tggtttcgcg
     5701 cagacggccg atctcgcgca gacaggaggg gatctggtgc gccgcggcga tgtagacggc
     5761 gaagtccccg gccgcaatca ggcggcacga ttccggcagg gaggcgatct cgcgcgttag
     5821 ggtctcgcac ggcacgggcg cgatgatcgg atcggcgggg agcatcggcg ggttcagcga
     5881 aataaagcgg cgcgtgcggt gcgtttcgcg ctcggccagc aggtaggaac ggaaccgcat
     5941 gtagcggatg agctgcacgt catccagcga aaacggctcc agcgcttttg gggaaagcgg
     6001 ggttccgatg cgcacggtga ttttccatcc ggtcttgttg gcgatctgcc gcggcagcag
     6061 cgaggtgcgg aaacgcggat gcaaaaaccc cgctatctgg aagaacacgt tgttgcggcc
     6121 ggggaaaaac atcgggacca cggtggcgtt ggttttgcgc gtcagcgcgg cgatgcttgc
     6181 gctccaggca gggtcgcgca cacgcagggt gcggcggtcg aaactggaga cctcgccgga
     6241 ggggaagacg atcaggaggt gaccttcctt cagccaggag agactctcct tgagggggcg
     6301 cagattcgcc ttggccgaat ttttcgaccc gaacggatcg actaaaatga aatcgccctg
     6361 catctccgga atggcgccga gcaggtaatt ggccatggct ttaacgtcgg ggcgcacacg
     6421 ccgcagcagg gagaggagga tgatcccttc gatgccgccg aaaggatggt tcgcgaccac
     6481 gacgaccgcg ccttctttgg ggatgcgggc cagatccgct tccgaaaagt cggttcggat
     6541 tccgatcgcc tgcagggcgc ggtcacagaa ggggatgtct ttgggcatcc gccatgtgcg
     6601 cgcatagatc ttatcgcagg cgcgcagacc ggtgatgctt tcaaccggac gtttcacgat
     6661 tctcaccagc aggcgcaaca ggcgggtgtg gaaggtgtgg acaggcagtt caaaaatacg
     6721 gggcgcctga ttttcggtgc ggtcagtatc catagtcagc ctccggagat tttgggtggt
     6781 gcgcgagtgc gagtacgaac gcgaggggga gtccgatgcc gcggaccaac agcgggtgcc
     6841 acacggcggt taccgcgaac gcggccaacg cgccagccgc gccccatcgt ccggaacggg
     6901 tcgccgcgcc tagagcggac gccagcataa gcagaaacgc gaagagcgcg ggcagaccgc
     6961 acgatgcgcc caatacgagg tacagattct ggatgtcggg ttccgcgggg ccggtcgcat
     7021 tcggaacctg tccgtaaaag gagccgatcc gtttctgata gtgaccgggg cccacgccca
     7081 gccacggatg ggtcaggatc atgccgacgg ctgcctgcca ctccggataa cggcgggtcg
     7141 ggagtccggc cgaatcatac aaggcaaccg attcgaaatg gcaaaggtcg ttttcacgcg
     7201 gaagattctt caggacttca gtctgccaca gcattaacgc cagcatgacg ggcaggaaca
     7261 cgcgcgcgcc gcgcgttgcg gctatcgcgg cgatgacgac gacgacagcc cagtaggagg
     7321 ctccggagag gttgaccgca agaccggtca gcagcagcag cgcacaggcg atccgcgtcc
     7381 agatggcgcg gcctgatgcg agcgcgccga ccgcgcacag cggcagggcc agcgcaagga
     7441 agccgcccag cacattgcgg ttcccgaatg tgccgcccac cagcaacgga ctttcccggc
     7501 cgccgagcac gttcacgatg atatcgagcg tcttgggttc ggcgaacagg cggatcgagt
     7561 cgtggccttc cggcgcgaga tattggatca cggccaaccc gagcaccacg gcggtcactg
     7621 cgccgagcgc gatgaaaccg ggcggcacac gagaggcggc gggggatgcc gctccctctg
     7681 cggatgcgtt gctgaaaagc cgttccttgc ggaggagcgc ggtgaacaca acgggagcga
     7741 tgataaaata ttcaactccc tgcacccatt ctttgagcgc gtccatccgg tccgttgccg
     7801 cggacagcgc cgcgcccgac gcgaggatga agagaaggtg cgtccagtgc ggccgcaggg
     7861 aaagcgcctt ttttccgtcc cgcgccaaca ggcactccag cacaaccagc gcggcggcac
     7921 aggccagaag cagatccgcc actccgatgc gcaaagaggg aatcagcgac cactgggtgg
     7981 gggcggcgca caatgcggcg agggcgaacc ccgggaaggc ggcacgggac aacggtcgaa
     8041 tgtacgcgcg ggtcattggc gcacacctgt tttttcgata tcggtcggga cgaaacgccg
     8101 gtcgctgctg agcaggatct ggtcaagccg cacaccgtcc tcgcggttca gaaaggtgag
     8161 ggtgtgcctg ccccgggtga gccggagcgg gggggcggtg cgcgaaaccg gatatttgac
     8221 ccagtgccag gttttgtagg tggcgtcctc cccgaacagg aaaggtggct ggtcatccac
     8281 ccgcacggtg aacgagttgc tgcactcgcc ctcccaccag acgcggcacc agagggtgaa
     8341 aaccccgtct tccgggatat ccagatcaaa gccggccact cccgcattga ccttgggcgg
     8401 attccccgca ccctccgcga tttcgagata tgcgccggag gccccctcga ttccgttgga
     8461 gaccacggtc acgggaggtt cgattcggtt ggcggtttcg gcctcccagc agatccgcac
     8521 ccctttcgcc tgagcacggc agatcaggaa gagacccgcg agcagaatca tccgttgcat
     8581 agaagtcata gtccgcagta tacggcattc cgtaccaatc ggcaacagga agggaggtcg
     8641 ttcagggcct gcgggggcaa aaaaa
//
LOCUS       DAOSLE010000118         8609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7671_length_8609_cov_7.878069, whole genome shotgun sequence.
ACCESSION   DAOSLE010000118 DAOSLE010000000
VERSION     DAOSLE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7671_length_8609_cov_7.878069"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1435
                     /locus_tag="PK576_08185"
     CDS             287..1435
                     /locus_tag="PK576_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61609.1"
                     /translation="MRTMLFACVAGAVLTTRAQVEAVWLTHRSATPEKTVVSWKSVTP
                     GPSRVAYGADAACGQTASVAGERSVHHVEIDTPQRGATVFYRVETGDQKSEVHSFQTC
                     PEDGVRVAIVGDWGYANRPDLTALKADKPNILMTVGDNVSDVINRKQPGDITNIRPYI
                     GLLRSEAAFFASTLFMPILGNHDKQVGPRVNKRPGSSKETYDIDATAYLSVFALPEPG
                     WRWAFTIPQADVTFLALDMHHLSDFGTTFQTSHDHRQGSEQFAWYDEQTRNLKTGFAV
                     TLLNADSRCRNREKGAWHQMFSRGTIFVTGYGYYAERAVERNGFPYYNTCVARPGDVY
                     RDPLAVVCERVSSYMLLTFDKAKGQMTVDLKRLDGSLIDRQVYHAKPR"
     gene            1521..2855
                     /locus_tag="PK576_08190"
     CDS             1521..2855
                     /locus_tag="PK576_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61610.1"
                     /translation="MTWTRRDFLSASAGAALAWRSVAAQQPVTAAREGWLEAFRAAGF
                     DPAAAGNSVFVVCGDVHFPQYGQHLPSQIEAWNAMRPAPQFVALLGDNVCNVSSSFGH
                     TPDAKGLERARKELEGLRAETAKLRKEIPLKLVIGNHDTLPRETDAAFFRKVFPACRP
                     YEAFEVSGICFQIWNGAHDGAIDPAQRVWIGKQCAGLPRDKSVVILVHQPSLGMTERE
                     RGIPAMVRAHFSEHTGPLWLLAGHVHSNSIREFALPRTRITQATHVKSVDGYWIYGMR
                     DGRITARVYCPVAGACKAQAMPDPARPARPIPLPFEGRDDVVWRLLIGEDATATKTAF
                     VRGKGGDCGTWWFYVNELVYKLPLASRGRGATRFAVLAALSKHRKTGELPKVYASGSD
                     GDWIETPLLESRGAEHLFAIPEGLRKSRELYVRIQSYGYGADTCVGGFALCR"
     gene            2905..4845
                     /locus_tag="PK576_08195"
     CDS             2905..4845
                     /locus_tag="PK576_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE61611.1"
                     /translation="MNTIKRKFLCRRGMMLLVVLGTGAAVQAATVLVEAEGFANAGGW
                     VIDQQSMDQMGSPYRMAHGLGVPVADAETSVQVPEAGRYRVWVRTRDWVATWGAPGAP
                     GAFRVLINGRALDPVFGTQRAEWHWQPGGEIALPVGPVTIALRDLTGFNGRCDAILLT
                     TDVGLKPPDGGGELAALRRQWLGLPDAPEDAGEFDLVVVGGGMAGCCAAVSAARLGCR
                     VALIQDRPVLGGNNSSEVRVGLSGQIRQQPYPRLGELVDEIGPVGHWNLYDAKRAPEL
                     PRSREVLEIIARHPEKKQHNAGPASNYEDERKRRVVEAEKGVRLFLATRVVGVERVGG
                     RIVSVTGRDIVTNRERRFRGRLFADCTGDAGVGALAGADFRVGREARGETGEGLAPER
                     ADTLMMGVSVQWYSETTDGPAPFPDCPWALPFTDQTVQALKRGDWDWETGMTRDSVTE
                     IEAIRDHALRATFGNWAYLKNHSAKRQAFANQRLAWVAYIGGKRESRRLLGDVILSQQ
                     DITGQRPYPDACVTTTWPLDLHYPHPENEKHFPGMAFRAVSKSEGIKPYAIPYRCLYS
                     RNIENLFMAGRNISVTHVALGSVRVMRTTGMMGEVVGMAASLCIRHDTTPRGVFETRL
                     EELRERMRRGVARGEGQASACP"
     gene            complement(4917..5606)
                     /locus_tag="PK576_08200"
     CDS             complement(4917..5606)
                     /locus_tag="PK576_08200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE61612.1"
                     /translation="MERAAFLFDIDGTLLYARGIGRAAFGTAFETAYGVPYPDLAGLT
                     FVGATDSNVVRTMAATCGVASTPAREEHFFLLLTQAIDAGLAQTKPLVYPGVPALLDR
                     LCKEGYPLGLITGNIRATAWAKLRHAGLDAAFCFGAYGDDDPDRNVIARLALQRVPHG
                     VRARVLIGDTPLDIQAAHANGLIAVAVATGWVTADALAAAGADLVLDDFSDTETVLAQ
                     LKALLASCNPG"
     gene            5701..6648
                     /locus_tag="PK576_08205"
     CDS             5701..6648
                     /locus_tag="PK576_08205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE61613.1"
                     /translation="MGNMRRRNFLKVTFGALTVGPVVARAAAFGAYDRVRLGRSGIET
                     SRLCFGTGYKAFNRVSEQTRLGHDAFVALLRAGYERGIRCFDAADLYGSHRPLAEALK
                     PFPRDRYTLISKIWWRKGGVPEEDKTAVPEQIRRFLKELKTDYVDIVQLHCLTDGRWQ
                     TELADRMEALEACKKAGMIRAHGVSVHGFKALQACATSPWVDVAHVRLNPYGVKMEGT
                     VEANLPVIRAMRGKGQGIIAMKLLGEGAFSHDGEKINASLAFALRSGLADVLNIGFMS
                     TAQIDDIAARIAAVTPNAGGTRNPHPDPLQGAGEQTTSP"
     gene            complement(6826..>8609)
                     /locus_tag="PK576_08210"
     CDS             complement(6826..>8609)
                     /locus_tag="PK576_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOE61614.1"
                     /translation="TISGGTLFAQGKNGGADIGQGSNGTVSGANTFTGGAVHLANNSI
                     SVAPTDGSQRVWCMTVTNLTPHALIVVTGLDPYGMNDLVADNAGKLRLWLPNGNYTFT
                     AGGAHYAATIDSADTTATLSALTPPDSDAYITFSSANTFEVKPKQGKSWNGILYYSTD
                     TTFWSVFTTDGATSATDGAGEHKLYLCGSGNSYITGNPDGPPWSITATAPVTCSGNLE
                     TLLDHAKVNAGEHPAMAPYCFSYLFKGCASLVSAPSLPATNLTNHCYYNLFNSCTGLT
                     NAPALPATTLPERCYNGMFNGCTNLTQAPALPATTLGKSSYGYMFYKCTGLTSAPALP
                     ATTLANECYKNMFMDCTALTSAPALPATTLASACYDHMFSKCTGLTNAPTLPATTLAP
                     SCYINMFSDCIGLMRVPELPAMNLAESCYNQMFRGCTNLTRPPELPATTLAVKCYYYM
                     FYKCTGIELNTAGPGMPWSIPANATAAPDWNRRMFYYTGGTLTGGPAIGTTYYLASGL
                     PDAPAFPTDGTGFVIGNGTATLAIINAEAGLWYTVYATDDLNATWQPVPGQSIRATGS
                     HVAFTFDIDTATVPRRFFKVAASLTTP"
BASE COUNT         1541 a         2485 c         3012 g         1571 t
ORIGIN      
        1 tgtggttaaa tggtcgatcc cgatagcgat agcgatcccg accccgattt ggatgaaacc
       61 ttgaaaccct gaaacctgaa accgtgaaac cctgaaaccc tgaaaccctg aaaccctgaa
      121 accgtgaaac cccgaaaccc agaaacccac gaaagtgtcc cgtcaggata atccggggtt
      181 tacctgtgcg caacggtttt gttatgcttt ttgcagatga ggtggtgtgg cgcgggatcc
      241 ggaaaaggct ttccgatgac gaaaaacaag aagaggaggg gaagggatga ggacgatgct
      301 gttcgcctgt gtggcgggtg cggtgctgac aacgcgggcg caggtggagg cggtgtggtt
      361 aacgcaccgt tcggccacgc cggaaaagac ggtggtgagc tggaagagcg tgacgccggg
      421 gccgtcacgc gtggcctatg gtgcggacgc ggcctgcggg cagacggcgt ccgtcgcggg
      481 tgagcggagc gtgcaccatg tggagatcga cacgccgcag cgcggggcca ccgtgttcta
      541 ccgggtggag accggagacc agaaatcgga ggtccactcc tttcagacct gtccggagga
      601 cggcgtgcgc gtggcgatcg tgggtgactg gggatatgcg aaccgtccgg atctgaccgc
      661 gctcaaggcg gacaagccga acatcctcat gaccgtgggc gacaatgtgt ccgatgtcat
      721 caaccgcaag caacccggcg acatcacgaa catccggccc tacatcggcc tgctcaggtc
      781 ggaggcggcg tttttcgcgt ccacactctt tatgccgatt ctcggcaatc acgacaaaca
      841 ggtcgggccg cgcgtcaaca agcggccggg ctccagcaag gagacgtacg acatcgacgc
      901 cacggcgtac ctgagcgtgt tcgcgctgcc cgagccgggg tggcgctggg ctttcacgat
      961 cccgcaggcg gacgtgacct tcctggcgct ggacatgcac cacctgtctg atttcggaac
     1021 gaccttccag accagtcacg accaccggca gggttccgag cagttcgcgt ggtacgatga
     1081 acagacgcgt aacctcaaaa ccggattcgc ggtgacattg ctgaatgccg acagccggtg
     1141 caggaatcgc gagaaaggcg cgtggcacca gatgttcagc cgcggcacga tctttgtgac
     1201 gggatacggg tactatgccg agcgtgccgt tgaacgcaac gggttcccgt actacaacac
     1261 ctgcgtggcc cggccgggcg atgtctaccg cgatccgctg gcggtcgtgt gtgagcgggt
     1321 ttcaagctac atgctgctga cctttgataa ggccaagggg cagatgacgg tggacctcaa
     1381 gcgtctggac gggagcctga tcgaccggca ggtttaccac gcgaaaccgc ggtaagccgc
     1441 cgtgcggccg cgccgcggat atccgcttgg cacaaccatc aagatgtttt gatgaggacg
     1501 gaaaaaaggg gggggagcac atgacatgga cccggcgtga tttcttgagc gcttcggccg
     1561 gggcggctct ggcttggcga tccgttgcgg cgcaacagcc ggttacggcg gcgcgggagg
     1621 gatggctgga ggccttccgc gcagcggggt tcgatcccgc tgcagcggga aacagcgtgt
     1681 tcgtggtgtg cggggatgtg cattttccgc agtacggcca acacctccct tcgcagatcg
     1741 aggcatggaa cgccatgcgg cctgcgccgc aattcgtggc tctgctgggc gacaacgtgt
     1801 gtaacgtttc gagtagtttc gggcacacgc ctgatgcgaa ggggctggag cgcgcacgga
     1861 aagagttgga ggggctgcgc gcggagaccg ccaagctccg caaggagatt ccgctcaagc
     1921 tggtgatcgg caaccacgac accctgccgc gggagacgga tgccgcattc ttccggaagg
     1981 tctttcccgc atgccggccc tacgaggcgt tcgaggtttc cggtatctgt ttccagattt
     2041 ggaacggcgc gcatgatggc gcgatcgatc cggcgcagcg tgtctggatc gggaagcagt
     2101 gcgctggcct gccccgcgac aaaagcgtgg tgattctggt gcatcagccc tcgctcggga
     2161 tgacggaacg cgagcgcggc atccccgcca tggtgcgggc ccacttttcg gaacacaccg
     2221 ggccgctctg gttgctggcg ggccatgtgc acagcaacag catacgcgag ttcgcgttac
     2281 cgcgtacccg gatcactcag gccacgcatg tcaagagtgt ggacggctac tggatctacg
     2341 gcatgcgcga cggccgtatc acggcgcggg tgtactgtcc ggtggcaggg gcgtgcaagg
     2401 ctcaggccat gcccgatccg gcgcggcccg cacggccgat cccgctgccg ttcgaggggc
     2461 gcgacgatgt ggtttggagg ctgctgatcg gtgaggacgc gacggcgacc aagacggcct
     2521 ttgtgcgcgg caagggcggc gattgcggca cctggtggtt ttatgtgaac gagctggtct
     2581 ataaactgcc gctggcgtcc cggggccgcg gggccacgcg cttcgccgtt ctggcggcgc
     2641 tgagcaaaca tcgtaagacc ggggagctgc ccaaggtcta cgcttctggc agcgatggcg
     2701 attggatcga gacaccgctg ctggaatcgc gcggggccga gcatctcttt gccatacccg
     2761 aggggttgcg taagagccgc gagttgtatg tgcggattca gagctacggg tatggggcgg
     2821 atacctgcgt tggcggattc gcgctgtgcc ggtgagtcgg tcacgcggct gcgggaagga
     2881 tggcatcgat tacggaaagg gcttatgaat acaataaaaa gaaaattcct gtgccgcagg
     2941 ggtatgatgt tgctggtggt tttggggact ggtgcggcgg ttcaagccgc gaccgtgctg
     3001 gtcgaggcgg aggggttcgc gaacgcaggc ggctgggtca tcgaccagca gtccatggac
     3061 cagatgggtt cgccgtatcg gatggcgcac gggttggggg tgccggtggc ggacgcggag
     3121 acctcggtgc aggtgccgga agcggggcgt taccgggtgt gggttcgcac acgtgactgg
     3181 gttgccacat ggggcgcgcc cggcgcaccc ggcgcgttcc gggtattgat caacggccgt
     3241 gcgctcgatc cggtcttcgg cacacagcgc gccgagtggc actggcagcc gggcggggag
     3301 atcgcgttgc cggtcggccc ggtgacgatc gcgttgcgcg atctgaccgg attcaacggg
     3361 cggtgcgacg cgattctgct gaccacggat gtcggcctga agccgcctga tggcggcggg
     3421 gagttggccg cgctgcgacg gcaatggctg ggcctgccgg acgcgccgga agacgcgggg
     3481 gagtttgatc tggtggtcgt gggcggaggc atggccggat gctgcgcggc tgtgagcgcg
     3541 gcccgtctgg gttgccgcgt cgccctgatt caggaccgtc ccgtattggg cggaaacaac
     3601 agttccgagg tgcgggtcgg cctttccggt cagatccgtc agcagcccta tccgcggctc
     3661 ggcgaactgg tcgacgagat cggtccggtc ggtcactgga acctgtacga cgccaaacgt
     3721 gcgccggaac tgccacgcag ccgcgaggtg ctggagatca tcgcgcgtca ccctgaaaag
     3781 aaacagcaca acgccggtcc cgcgagcaat tacgaggacg agcgtaaacg ccgcgtggtt
     3841 gaagccgaga aaggggtgcg tctgtttctc gcgacgcgcg tggtcggcgt ggagcgggtg
     3901 ggcggacgca tcgtgtctgt gaccggccgg gacatcgtga ccaaccgcga gcggcgtttc
     3961 cgcggcaggc tgttcgccga ctgcacgggc gacgcgggcg tgggggctct ggcgggtgcg
     4021 gatttccgtg tcggccgcga ggcccggggc gagaccggtg agggtttggc gccggagcgg
     4081 gccgatacac tgatgatggg tgtgtcggtc cagtggtatt ctgaaaccac ggacgggccg
     4141 gcccctttcc cggattgccc ttgggccctg ccgttcaccg atcagaccgt gcaggccctg
     4201 aagcgcggcg attgggattg ggagaccggc atgacgcgcg actccgtgac agagatcgag
     4261 gcaatccgcg accatgcctt acgtgccacc ttcgggaact gggcctatct gaaaaaccac
     4321 agcgccaaac ggcaggcgtt cgccaaccag cggctggctt gggtggccta tatcggcggc
     4381 aagcgggaaa gtcgacgcct gctcggcgac gtgatcctct cccagcagga cattaccggg
     4441 cagcgcccgt atcccgatgc gtgcgtcacc acaacctggc cgttggacct gcactatccg
     4501 caccccgaaa acgaaaaaca ctttccgggc atggcctttc gtgccgtttc caaatccgaa
     4561 ggaatcaaac cctacgcgat tccgtaccgc tgcctttaca gccgtaacat tgagaacctg
     4621 tttatggcgg gccgcaacat cagcgtgacc catgtggccc tgggttcggt gcgcgtgatg
     4681 cgcacgaccg gtatgatggg cgaggtggtc ggcatggcgg cttcgctttg catacgccat
     4741 gacaccacgc cgcgcggtgt gttcgagacg cggctggaag agttgcggga gcggatgcgg
     4801 cgtggtgtcg cccgggggga ggggcaagcg tctgcttgcc cgtgaggacg gttctccgaa
     4861 ccgtcagcgg acgcctcggg aatgcgtccc tgccttcaaa aaatcgtcca gaaaaattag
     4921 ccgggattgc aggaagcgag cagtgctttc agctgggcca gcacggtttc ggtatcggaa
     4981 aaatcatcca gcacaagatc ggcgccggcc gcggcgagcg cgtcggctgt gacccatccg
     5041 gtcgccaccg ccaccgcgat gaggccattg gcgtgggcgg cctgaatatc gagcggggtg
     5101 tcgccgatca gtacacgggc gcggacccca tgcggcacgc gttgcagggc gagacgcgcg
     5161 atcacgttac ggtccggatc atcgtccccg tacgcgccga agcagaatgc cgcgtccagc
     5221 cctgcgtgtc gtaacttggc ccaggcggtc gcacggatgt tgccggtgat caatccgagc
     5281 gggtatcctt ctttgcaaag ccgatccagc agggcgggca cgccaggata gaccagcggc
     5341 ttggtttgcg cgagcccggc atcgatggcc tgcgtcagca gcagaaagaa gtgctcctcg
     5401 cgcgcgggcg tgctggccac accgcacgtc gcggccatgg tgcgcaccac gttggagtcg
     5461 gtcgcgccca cgaaggtgag ccccgcgaga tccgggtaag gtacgccgta cgcggtctcg
     5521 aacgcggtgc cgaacgcggc ccgtccgatt ccccgcgcat agagcagggt tccgtcaata
     5581 tcgaataaaa aagcggcacg ttccatgatg ggattgaaaa ccgggcccgt ccgggctaca
     5641 atgtgcctga actatatcac acgcgccggg tacaggcgac gggaaacaga ggggtgaggc
     5701 atggggaaca tgcggcgcag gaattttctc aaggtaacgt tcggcgcgct gaccgtcggc
     5761 ccggttgtgg cgcgcgcggc ggcgttcggc gcgtatgacc gggttcggct cggacggagc
     5821 ggcatcgaga cttcgcggct ctgtttcggc accgggtaca aggcgttcaa ccgcgtctcc
     5881 gagcagacgc ggctggggca tgacgccttt gtggccctgt tgcgcgcggg gtacgagcgg
     5941 gggattcgct gtttcgatgc cgccgacctg tacgggtcgc accgaccgct ggccgaggcg
     6001 ctcaagccct ttccgcgcga ccgctacacg ctgatctcca aaatctggtg gcgcaagggc
     6061 ggggtgccgg aggaggataa gaccgcggta ccggagcaga tccggcgttt cctgaaagag
     6121 ctgaagaccg attacgtgga tatcgtgcag ttgcactgcc tgaccgacgg gaggtggcag
     6181 acggaactgg ccgaccggat ggaggcgctt gaagcgtgca agaaggccgg aatgatccgc
     6241 gcgcacggcg tgtccgtgca cgggttcaaa gcattacagg catgcgcgac ctctccctgg
     6301 gtcgatgtgg cccacgtccg actcaatccg tacggcgtca agatggaggg cacggtggag
     6361 gcgaatcttc cggtcatccg cgcgatgcgc gggaagggtc aggggatcat cgccatgaag
     6421 ttgctgggcg agggtgcctt ctcgcatgac ggggagaaga tcaacgcttc gctcgcgttc
     6481 gcgctccgca gcggactggc cgatgtgttg aacatcgggt tcatgagcac cgcacagatc
     6541 gatgacattg cggcgcggat cgccgccgtg acccccaatg cgggtggaac ccgcaaccct
     6601 caccccgacc ctctccaagg ggcgggggag caaacaactt ccccgtaaac cgccgccggg
     6661 caagcagacg cttgcccctc ccgttgaccc gcgactggcg acttgcgtca agcaaacaaa
     6721 acgtcgcagg tcgaatgtcg cgggtcgcgg gtcaacgtac acgaaaacgt gtgcgatccc
     6781 gatcccggtg cttcgaaaac ttatcacttc cacgcttcac agcgcctagg gcgttgtgag
     6841 gctggctgcc accttgaaga agcggcgggg gactgttgcc gtgtcaatat cgaatgtgaa
     6901 ggcaacgtgg ctgccggtcg cgcggatgct ctggcccggt accggctgcc aggttgcgtt
     6961 gaggtcgtcc gtcgcataga ccgtgtacca gaggccggct tcggcgttga tgatcgcgag
     7021 ggtggccgtg ccgttgccga tgacgaatcc cgtgccgtcc gtggggaagg ctggcgcgtc
     7081 gggaaggccg gacgcgaggt agtacgtggt gccgatcgcc gggccgccgg ttaaggtgcc
     7141 gcccgtgtag taaaacatcc gtctgttcca gtccggtgcc gccgtcgcgt tggccgggat
     7201 gctccacggc attccggggc ctgcggtgtt cagctcgatg cccgtgcatt tgtagaacat
     7261 gtagtaatag cacttcacgg ccagagtcgt cgccggcaat tccggcggac gcgtcaggtt
     7321 cgtgcaaccc ctgaacattt ggttgtagca gctctcggcc agattcattg ccggcaattc
     7381 cggcacgcgc atgagcccaa tgcagtcgct gaacatgttt atgtagcaag agggtgcaag
     7441 cgtcgtggcg ggcagcgtcg gcgcgttggt cagccccgtg catttggaga acatgtggtc
     7501 gtagcaggct gacgccagtg tcgtggcggg cagtgccggt gcgctcgtca gtgccgtaca
     7561 gtccatgaac atgttcttgt agcattcgtt agccagcgtc gtggccggca gtgccggcgc
     7621 gctcgtcagt cccgtgcatt tgtagaacat gtagccgtag ctggatttgc ccagcgtcgt
     7681 ggcgggcagc gctggcgcct gcgtcaggtt cgtgcatccg ttgaacatgc cgttatagca
     7741 tctttccggc aatgtcgtgg ccggcagtgc cggcgcgttc gtcagccccg tgcaactgtt
     7801 gaacaggtta tagtaacaat gattggtcag atttgtcgcc gggagcgatg gtgcgctgac
     7861 gagcgacgca cagcctttga agaggtacga gaagcagtag ggcgccattg ccggatgctc
     7921 gcctgcgttc accttcgcat ggtcgagcag ggtttcaagg ttgccggagc acgtgaccgg
     7981 cgccgtggcg gtaatgctcc agggggggcc atctgggttg ccggtaatgt aactgttgcc
     8041 cgatccgcag agatacagct tatgctcccc ggcaccgtcg gtagccgagg tcgcgccgtc
     8101 cgtggtgaag accgaccaga acgtcgtgtc ggtggagtaa tacagtatcc cgttccatga
     8161 ttttccttgc ttcggcttga cctcgaaggt gttcgcgctt gagaaagtga tgtacgcgtc
     8221 cgagtctggg ggcgtcagcg ctgacaaggt cgctgtcgtg tccgcactgt cgatggtcgc
     8281 ggcgtagtgt gcgccgcccg ccgtgaaggt gtagttgccg ttgggcagcc agaggcggag
     8341 tttgccggca ttgtctgcaa cgaggtcgtt catgccgtag gggtcgaggc cggtgacgac
     8401 gatcaacgca tggggcgtga ggttggtcac cgtcatgcac cagacgcgtt gtgagccgtc
     8461 cgtgggcgcg acggagatgc tgttgttcgc caggtggacc gcgccgccgg tgaaggtgtt
     8521 cgcgccggag accgtcccgt tgctgccctg tccaatgtcc gcgccgccgt tcttgccttg
     8581 cgcgaagagc gtgccgccgg agatggtca
//
LOCUS       DAOSLE010000119         8441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7852_length_8441_cov_6.171953, whole genome shotgun sequence.
ACCESSION   DAOSLE010000119 DAOSLE010000000
VERSION     DAOSLE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7852_length_8441_cov_6.171953"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PK576_08215"
     CDS             <1..563
                     /locus_tag="PK576_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE61615.1"
                     /translation="LAERIPEIDAKKSVATGCSRLAKATLLATVRDERFAVCVPNQTG
                     GGGVPLAKRDFGENVSTEMNMFHHWYCKAYGKYIDNEQAMKFDQHLLVAAVAPRRILV
                     QGYNQGWFDTKGEFLACRAASAAWEFLGLPGLPAGDFPANYDTGRIGTHLGYVRRGGQ
                     HGISGYDWVWMLDFADVAFGKLRDAP"
     gene            1045..2169
                     /gene="prfB"
                     /locus_tag="PK576_08220"
     CDS             1045..2169
                     /gene="prfB"
                     /locus_tag="PK576_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE61616.1"
                     /translation="MVMEFDEVKERLETMREKLDEMRGYLDIANRRALLAELEEAAAR
                     PDFWNDPNAAQEVIGKTNAQRAFVRPFDELVRQIEDGEVMLELAVAEKEGEQRQQALA
                     ELTNMFGKADVMFSKLRLQSLLGGKLDACNAYLTLHSGAGGTESCDWADMLFRMYRMY
                     CEDNGFEMEVLDSQPGDEAGLKSVTFLVSGPYAYGYLKAERGVHRLVRISPFDANKRR
                     HTSFCSLDVVAELTDEIEVEIKEEDLRVDTYRSSGAGGQHVNKTDSAIRLTHMPTGIV
                     VAVQSERSQHKNRSKAMSILKAKLYEYYEDKKRKEMERFYGEKGEIAWGSQIRSYVFQ
                     PYTMVKDHRTDEQTSNVQAVMDGRLQPFIEAYLRQNAVKV"
     gene            2267..3595
                     /locus_tag="PK576_08225"
     CDS             2267..3595
                     /locus_tag="PK576_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HOE61617.1"
                     /translation="MRMTFGQAKEVLARHGQMQVMRFWRSLDRAARGALLAQIASIDF
                     SEVARMRKLLAQGTRAVPVTPRPAPVVTWTRRMRAEALAKGQEQLRKGRVAALLVAGG
                     QGSRLGYEGPKGAYGIGPVSNLPLFYFHARKLVRLARLYGKPVPFYIMTSEANDAATR
                     ACFEDYNYFGLHPNDVVFFKQGLWPALDEAGHLILETPGRIFMSPDGHGGTLTALDRN
                     GCLQDMAARGIRNVFYFQVDNPLVEIADPAFVGLHVLRRADLSLKLCRKRSPTEKMGA
                     VSALDSGRFAMIEYSEMTPKQAARFRYGSPAIHIFSIAFLNREARKNLPLHLAHKKIA
                     CVDEAGRTVMPDAPNGYKFEKFIFDVLPDAKTVVNLAFDREEEFSPVKNAVGEDSPAT
                     CRMALQAKWRRWLAKAGVIVPESIPVEIDPAYAGGAEELKRGLLVQDIMI"
     gene            3697..3861
                     /locus_tag="PK576_08230"
                     /pseudo
     CDS             3697..3861
                     /locus_tag="PK576_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            3854..4075
                     /locus_tag="PK576_08235"
     CDS             3854..4075
                     /locus_tag="PK576_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61618.1"
                     /translation="MIDIETILNKACDILTEEARMTGFKSSPLFEQRVRNVLAALMPD
                     NFGIKVDMSPPAQAFPDGNHQKFCFWMNR"
     gene            4092..5651
                     /gene="gpmI"
                     /locus_tag="PK576_08240"
     CDS             4092..5651
                     /gene="gpmI"
                     /locus_tag="PK576_08240"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOE61619.1"
                     /translation="MRPVVLIVRDGWGLNPREDGNAVFAAQTPVIDRLKARYPWTRLA
                     CSGEAVGLPEGYQGSSEVGHLNMGAGRVVVQELKRIDDGLSTGELFKSAKWAALIANW
                     KEHKSALHFMGLLQDEGVHAHQEHLFKLMRRAREEYPEGRIVVHPFLDGRDTPPRSTL
                     EYLARLEKEMAAVGNCVIGTVQGRYYAMDRSKNYALTDTAYACLTAGEGRKAATAAEA
                     VQESYDKDQTPDNTPMVDEYIPPYVIGEYAGMKDGDSVMHFNYRQDRAIQLSQAFVCA
                     DYAGKRASSPRVRYLGFTRYWNEFSEYLLGAMGGDGGMDNLLGEVIAKAGLRQLRIAE
                     TQKFRHVTSFFNGKSTSPYPGEDQVDVPSRFDPATFASHPEMEAYNVTDELLKRLQDN
                     PYAFVAVNYANCDMVGHTGDFEATRRAVEVVDECIGRLLPRLFELDAHVLITADHGNA
                     EQMKDYETGANKTSHTLNEVECLYVAKDSAGVKLVEAGKLSDIAPTVLFLLDLPVPQE
                     MTAGNLIKSAG"
     gene            5936..7135
                     /locus_tag="PK576_08245"
     CDS             5936..7135
                     /locus_tag="PK576_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOE61620.1"
                     /translation="MRIVFLIAVFVVQSAVAAIKFSNSYYSPRNRERPIRRSTSLIVL
                     HTTEAPSSSALRKLRDLGECHYCVDERGIVYRIIDHRREAYHAGRSMWNGRANVDSFS
                     VGIEMCGYHNKPLNAAQFRSLSALIGELQYIYKIPDHQVLTHAHVAYGAPNKWHRRSH
                     RGRKRCGMLFALPAVRARLNLKSRPKSDPDVRARRLVVGDAYLANVLYGSTSVGQASA
                     VPTLRAAEENVIAKGRSAWDIARDAYNDPTTLYRYPDGSVKRGNQIANFKQIPAGTRV
                     TVRADVRENRSETYQVIGVNGRAQDIAGEEARSASTVYVYPDGTYARGSQLSAAAVLR
                     LPYGTRVLTGYAIGGPVTASRKATDICGNRWRSAETFFLISGALVPGNKVDDSRIPVG
                     TMLFFKN"
     gene            complement(7125..7676)
                     /locus_tag="PK576_08250"
     CDS             complement(7125..7676)
                     /locus_tag="PK576_08250"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOE61621.1"
                     /translation="MATVLLGVTGSIAAYKACELTRLLVKAGNDVHVVMTQAAMRFVS
                     PLTFRTLSRNPVPTDSFAEPETWMPGHISLAEHADILIIAPCTANVIAKLAYGLADDL
                     LTATALASRAPLVIAPAMNSGMWEHPATQANLGLLASRGVRLADAGTGDLACGTQGKG
                     RMAEPQVIFDLVSATLQSEALNS"
     gene            complement(7714..8307)
                     /gene="gmk"
                     /locus_tag="PK576_08255"
     CDS             complement(7714..8307)
                     /gene="gmk"
                     /locus_tag="PK576_08255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE61622.1"
                     /translation="MKPLFLVLSAPSGGGKTTLCDLLLQHYPEFCYSVSCTTREPRID
                     EEDGVDYYFLPMETFKRMIAEDRFLEYATVHDHYYGTLKEPVYDTLREGQCMLLDIDV
                     EGAHQIRTHVAGLPADNPLRQGYVDLFINPPSLEVLTERLQGRATDRQEVIEKRIRNA
                     QAEIARANEYMYQVTNDDLKTTFRRICDIINVRAGFI"
BASE COUNT         1748 a         2217 c         2772 g         1704 t
ORIGIN      
        1 accttgccga acgtattcca gagatcgatg cgaagaagtc cgtggccacc ggctgctcac
       61 gtctggcaaa ggctaccctt ctggccactg tccgggacga gcgtttcgcc gtatgcgttc
      121 ccaaccagac aggtggcggt ggtgtgcctc tcgccaagcg cgacttcggt gagaacgttt
      181 ccaccgagat gaacatgttc caccactggt actgcaaggc ttacggcaaa tacatcgaca
      241 acgagcaggc gatgaagttc gaccagcatc tccttgtggc ggcggttgcg ccgcgccgta
      301 tcctcgttca gggatataat caggggtggt tcgacacgaa gggtgagttc ctcgcttgcc
      361 gtgcggcatc tgccgcatgg gagttcttgg ggcttccggg tttgcccgca ggcgatttcc
      421 ctgccaacta cgacaccggc cgcatcggta cccatcttgg ttacgtgcgg cgcggcggtc
      481 agcacggcat ctcaggatac gattgggtat ggatgcttga cttcgccgat gtcgcgttcg
      541 gcaaactccg cgacgcgcca tgaaaccggg agtgccatca gcagacgcgg acgaatcgct
      601 cggtgatcgc gcggattttg ggcaaaagct cgtttgatcc gtacccgttc acagcgcagc
      661 aggcacactg cccgtgcacg aaaacgagaa cgtgtacggg gacgaggacg agtacgtgtg
      721 cggggaaacg attccgattc tgacagcgat gccttaggcc gggaggggag gggcaagcat
      781 ttgcttgccc ggacgagcag acgctcgtcc ccgtcatttg atttcatgat gttttgtggt
      841 ccggaatcgg gaacccggaa cccgaaacca agcacgatca cgggaaatcg ggtcgtttcc
      901 ctcttgcgga cttccgctgt ttaaccaccg tcaatacagc aggaacgcgc cctgaacgtt
      961 tcggtctgcg gacttgcact tgcggggttg gctcaatccc gatatagtaa cgctttcttt
     1021 ttctctacag tagtaagacg gacgatggtg atggaattcg atgaggtaaa agagcgtctg
     1081 gaaacgatgc gcgagaaact cgacgagatg cgcgggtatc tcgatatcgc gaaccggcgc
     1141 gccctgctcg cggaacttga ggaagcggct gcccgtcccg atttctggaa cgacccgaat
     1201 gcggctcagg aggtgatcgg caagaccaac gcacagcgtg cgtttgtgcg gccgtttgac
     1261 gagttggtcc gacagatcga agacggtgaa gtgatgctgg aactggcggt cgccgaaaag
     1321 gagggggaac agcggcaaca ggcgttggct gaactgacga acatgtttgg caaggcggac
     1381 gtaatgttct ccaaattgcg tttgcagtcg ctgctgggag gcaaactcga cgcctgcaac
     1441 gcctacctga cgctgcattc cggtgcgggc ggtacagagt catgcgattg ggctgacatg
     1501 ctgttccgca tgtatcggat gtattgcgag gacaacggtt ttgagatgga ggtgctcgac
     1561 tcccagccgg gggatgaggc agggttgaaa tcggtgacgt tcctggtttc cggaccgtat
     1621 gcgtatgggt atctcaaggc cgaacgtggt gttcaccggc tcgtgcggat cagccccttc
     1681 gatgccaaca agcgacggca cacgtcgttc tgttcgctgg atgtggtggc ggaactgact
     1741 gatgagattg aggtcgagat caaggaagag gacttgcgtg tggacaccta ccgctccagt
     1801 ggcgcggggg ggcagcatgt gaacaagacc gactccgcga tccgcctgac ccatatgccc
     1861 accgggattg tggtggcggt gcagtccgag cggtcgcagc acaagaaccg ctccaaagcc
     1921 atgagcatcc tcaaggccaa gctgtacgag tattatgagg ataagaaacg caaggagatg
     1981 gagcgttttt acggcgagaa gggggaaatc gcatggggca gccagatccg ttcctacgtg
     2041 ttccagccct atacgatggt aaaggatcac cgcacggatg agcagacctc taacgtgcag
     2101 gcggtgatgg acgggcggct tcagccgttc attgaagcct atctcaggca gaacgccgtg
     2161 aaggtgtgaa gaagggcccg ccccctccgg ggggggcggg aataaacgaa agaggaaccg
     2221 gaacaccgca cgtttagagt gtaacgttca ggttccgggg agacgcatgc gtatgacatt
     2281 cggacaggca aaagaggtgt tggcacggca cggccagatg caggtgatgc gcttttggcg
     2341 aagtttggac cgtgccgcgc gtggcgcgtt actggcacag atcgcgtcga tcgatttcag
     2401 tgaggttgcg cgcatgcgga aactgttggc gcagggaacc cgtgccgttc ccgtcacgcc
     2461 ccgtcccgca ccggtcgtga cttggacccg tcggatgcgt gccgaagccc ttgcgaaggg
     2521 gcaggagcag ttgcgtaagg gacgcgtggc cgctttgctg gtggcgggcg gccaaggatc
     2581 gcgtctgggg tatgagggcc cgaaaggcgc ctacgggatc ggtccggtgt cgaatttgcc
     2641 gctcttttac ttccatgccc gcaagctcgt tcggcttgcg cgcctgtacg gcaaacccgt
     2701 gcctttctat atcatgacca gcgaggcgaa cgacgccgca acacgcgcct gtttcgagga
     2761 ttacaactat ttcgggctcc accccaatga cgtggttttc tttaaacagg gtttgtggcc
     2821 agcgttggat gaggcaggcc atctgattct ggagacgccc ggacgaatct tcatgagtcc
     2881 agacggtcac ggcggcacac tcaccgcgtt ggaccgcaac ggttgtctgc aagacatggc
     2941 cgcgcgcggg atccgcaatg ttttctattt tcaggtggat aatccccttg tggagatcgc
     3001 cgacccggcg tttgtgggtt tacacgttct ccgccgcgcc gatctctcgc tcaaactctg
     3061 ccgcaaacgt tctcctacag aaaagatggg ggcggtctct gcgcttgatt ccggacgctt
     3121 cgccatgatc gaatattccg aaatgacgcc gaaacaggcc gcgcggttcc gttacggcag
     3181 tcccgcgatc cacatctttt cgatcgcatt cctcaatcgg gaggcacgga agaacctgcc
     3241 gttgcatctg gcccataaga agatcgcctg tgtggatgag gcgggacgca cggtcatgcc
     3301 ggacgccccg aacggttaca agttcgagaa attcattttt gatgtgttgc cggacgcgaa
     3361 gacagtggtg aatctggcat ttgaccggga ggaggagttc tcgccggtca agaatgcggt
     3421 tggcgaggat tcgcctgcca catgcaggat ggcccttcag gcgaagtggc ggcggtggct
     3481 cgcgaaggcg ggtgtgatcg tgccggagtc gatcccggtc gagatcgatc cagcgtatgc
     3541 gggaggcgcg gaggaactta agaggggctt gttggtgcaa gatattatga tataattgat
     3601 ttgaagggag tcggtgtgac gacatgggtg atacctagaa tggagaagca gcggagaatc
     3661 aagagttctc tccggcacat ggctttggcg gacaggcgtg tggcggcacg aatacaatct
     3721 ttccctgaca gcttccgctt tttttgtggt ctccgggaaa ccgaacgaca gatagggaat
     3781 gcggtaccgc ccgttctggc ttggcacgtc gcccgcgctg tcgcggcgat attgcgggag
     3841 ggctcatctt cacatgattg acattgaaac catattgaac aaagcgtgcg acattttaac
     3901 tgaagaagcg cgcatgactg gtttcaaatc aagtccttta tttgaacaaa gggttcgtaa
     3961 tgtgcttgcg gctttaatgc cggacaattt cggaataaaa gtcgacatgt ctccgcctgc
     4021 gcaggcgttt ccggatggaa accatcagaa gttttgtttt tggatgaaca gatgaaggag
     4081 agttgcaaac gatgagacca gtagttttga ttgttcgtga cggatggggg ttgaatccgc
     4141 gggaagacgg gaatgcggtg ttcgcggcgc agacgccggt gatcgaccgt ttgaaggcgc
     4201 ggtacccctg gacgcggctg gcgtgcagcg gcgaggcggt gggtctgccg gaaggttatc
     4261 agggttcaag cgaggtcggg cacctgaata tgggcgccgg gcgcgtggtg gtgcaggaac
     4321 tgaagcggat tgatgacggg ctgagcacgg gcgagctgtt caagagcgca aagtgggcgg
     4381 cactgatcgc aaactggaaa gagcacaaaa gcgcgttgca ctttatgggg ctgctgcagg
     4441 acgagggtgt gcacgcgcac caagagcacc ttttcaagct gatgcggcgg gcgcgggaag
     4501 agtatcccga aggccgcata gtggtgcacc cgttcttgga tgggcgcgac acgccgccgc
     4561 gcagcacgct cgagtatctg gcgcggttgg agaaagagat ggcggcagtg ggcaactgcg
     4621 tgatcggcac ggtgcaggga cggtattatg ccatggaccg ctcgaagaat tacgcgctga
     4681 ccgacacggc ttacgcctgc ttgacggcgg gcgaggggcg caaggcggct acggcggcag
     4741 aagcggtgca ggagtcgtat gataaagacc agacaccgga taacaccccg atggtggacg
     4801 agtacatccc gccgtatgtg atcggcgaat atgcggggat gaaggacggg gattcggtga
     4861 tgcacttcaa ctaccggcag gaccgtgcga tccagttgtc gcaggctttc gtctgcgcag
     4921 attacgcggg taagcgcgcg tcgtctccca gagtgcggta tctggggttc acccgttact
     4981 ggaacgagtt cagcgaatac ctgctggggg cgatgggcgg tgacggcggc atggacaacc
     5041 tgctgggcga ggtgatcgca aaggccggtc tgcggcagtt gcgtatcgcc gagacgcaga
     5101 agttccggca cgtgaccagc tttttcaacg gcaaatccac aagtccgtat cccggcgagg
     5161 atcaggtgga cgtgccgagc cgctttgacc cggcgacttt cgcgagccac cccgagatgg
     5221 aggcgtacaa cgtgacggac gagctgctca agcggttgca ggacaacccg tatgcgttcg
     5281 tggcggtgaa ctacgccaac tgcgacatgg tggggcacac cggtgacttt gaggcgacgc
     5341 gcagggcggt tgaggtcgtc gacgagtgta tcgggcggct gctgccgcgt ctgttcgagc
     5401 ttgacgcgca tgtgctgatc acggctgacc acggcaatgc cgagcagatg aaggattacg
     5461 agaccggcgc caacaagacc agccacacgc tcaatgaggt tgagtgcttg tatgtggcca
     5521 aggactcggc gggggtcaag ctggtggagg ctggcaagct gagcgacatc gcgccgacgg
     5581 ttctgttctt actggatctg ccggtgccgc aggagatgac ggcaggcaac ctgatcaaat
     5641 cggcagggtg agaggctgta ggtaagaggt aaatggtgag tggtgagtgg taggagcgcg
     5701 cgtaaacgcg ctacgcagga cggcaggatt ggtagcagcg gcgccgggat aggggaaggc
     5761 gggagcgcgg gcgaacggga cggctcgccg agccgtccgc ggcggtttcg gcgaaaccgc
     5821 cctaccggtt gtaacacggc tttggtatga tagaatggcc tttgcgtgag cttgtggatt
     5881 gggggatggc ttgtttttat gggataaatg gaaaaggagg gatgacgggg gcgtcatgcg
     5941 gattgttttt ctgatagcgg tgtttgttgt ccagagcgct gtggctgcga tcaaattcag
     6001 taacagttat tacagtccgc gcaaccggga gcggccaatc cgcaggtcaa cgtcgctgat
     6061 agtcctgcac acgacggaag cgccttcaag tagcgctttg cgtaaattgc gcgacttggg
     6121 ggagtgccac tactgcgttg acgaacgcgg gattgtctac cgcatcatcg atcaccggcg
     6181 cgaggcatac catgcggggc gcagcatgtg gaacggacgc gccaacgtgg attcgttttc
     6241 ggtcggtatc gagatgtgcg ggtaccacaa caagccgttg aacgcagcgc aattccgctc
     6301 gctgtcggca ctgatcggcg agttgcagta catatacaag atcccggatc atcaggtgct
     6361 cacgcatgcg catgtggcgt acggggcacc aaacaaatgg catcgacgga gtcatcgagg
     6421 gcggaaacgg tgtggcatgc tcttcgcgct gcctgcggtt cgtgcgcggc ttaatctcaa
     6481 gtcgcgtccg aagagcgatc cggatgttcg tgcgcggcgg ctggtggtgg gggatgccta
     6541 tctcgcgaat gttctgtacg gttcgacgtc cgtggggcag gcaagtgccg tgccgaccct
     6601 ccgcgcggcg gaagagaatg tgatcgccaa ggggcgtagc gcttgggaca tcgcgcgcga
     6661 tgcgtacaac gatcccacca cgctataccg gtatcccgac ggatcggtca aacgcgggaa
     6721 tcaaatcgcc aatttcaaac agatccccgc aggcacgcga gtcaccgtgc gggcggatgt
     6781 gcgggaaaac cggtcggaga cgtatcaggt gatcggggtg aacggtcggg cgcaggatat
     6841 cgcgggcgag gaggcccgga gcgcgtcaac ggtgtatgtc tatccggacg gcacctatgc
     6901 gcgcggcagt caattaagcg ctgcggcggt tctgaggctg ccctacggga ctcgtgtgct
     6961 gaccggctac gcgattggcg ggccggttac ggcgagccgc aaagcaacgg acatttgcgg
     7021 taaccgctgg cgctccgccg agacgttttt tctcatctcc ggcgcgttgg ttcccggaaa
     7081 caaagtggat gacagccgga ttcccgtcgg cacgatgctg ttcttcaaga attgagcgct
     7141 tcggattgaa gtgtcgccga aacaagatcg aagatcactt ggggttcggc catacggccc
     7201 ttgccctgcg tgccgcaggc aaggtcgccg gtcccggcat ccgccaggcg cacgccgcgc
     7261 gatgcgagga ggcccagatt ggcttgtgtc gccggatgtt cccacatgcc ggaattcatg
     7321 gcgggcgcga taaccagcgg cgcgcgtgat gcaagcgcgg tggcggtcaa caggtcgtcc
     7381 gccagcccgt aagcgagttt ggcgatcaca ttggcggtac agggcgcgat gatcaggatg
     7441 tcggcgtgct ccgcgagcga aatgtgtccg ggcatccacg tttccggctc ggcgaacgaa
     7501 tccgtcggaa ccggattccg ggacagcgtg cggaacgtca gcggcgaaac gaatcgcatg
     7561 gcggcttgtg tcatcaccac atgcacatcg tttccggcct tcaccagcag ccgggtcagt
     7621 tcacaagcct tgtaggcggc gatcgaaccg gtcactccaa gcagtacggt tgccatactc
     7681 ctctcctttc ccgaaccttt ggcgcgaagc gcctcagatg aatcccgccc ggacattgat
     7741 gatgtcacaa atgcgccgga acgtcgtttt gagatcgtcg ttggtcacct gatacatgta
     7801 ctcgttggcg cgcgctatct cggcttgcgc gttgcggatc cgtttctcaa tcacctcctg
     7861 gcggtccgtg gcacggcctt gcagccgttc ggtcagcacc tccagcgagg gcgggttgat
     7921 gaagagatcc acgtaccctt gccgcaatgg gttatctgca ggcaggccag ccacatgcgt
     7981 gcggatctgg tgtgcgcctt caacatcgat gtcgagcaac atgcactggc cttcgcgaag
     8041 cgtgtcatac accggttctt tcagtgtgcc gtaatagtgg tcatgcacgg tggcgtactc
     8101 aaggaaccgg tcttccgcga tcatccgttt gaaggtttcc atcgggagga aatagtagtc
     8161 aaccccgtct tcctcatcaa tgcggggttc gcgggtggta caggaaacgg aataacaaaa
     8221 ctctggatag tgttgcagca acaggtcaca taaggtggtt ttacccccgc ctgacggtgc
     8281 ggacaacaca aggaatagcg gtttcatcac gattctcctt cacaaaacgc acacagtgta
     8341 cccgcttaca gggccggatc aaagtgcaaa tattggatgg gacctcggtt tccggttccg
     8401 taccatgaaa cacaagaaac agcagtcctg cggtttcccc t
//
LOCUS       DAOSLE010000120         8423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7877_length_8423_cov_6.190249, whole genome shotgun sequence.
ACCESSION   DAOSLE010000120 DAOSLE010000000
VERSION     DAOSLE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_8423_cov_6.190249"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1179)
                     /locus_tag="PK576_08260"
     CDS             complement(97..1179)
                     /locus_tag="PK576_08260"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOE61623.1"
                     /translation="MKKTVPLILFLILALVVMGPWLPAQATLQACAGSGAQPCRDIVF
                     KPLPGGEMRYGDTSRIPDRPFAKDPTVIRHNGRYLMYYSVRGYPPESRPADVPKERWG
                     WCGAIAESTNLVDWVRVSDILFQGPARAVCCGVAPCVRKFDGKIHLFAQGPPLVGSGR
                     MRDVLWHATSTDGIHFTYGPENPVFVPQNEWSIARAIDAEVYRVGDRMMLMFATRERP
                     NARIQQLGVAWAPYGSDYGASKWTELSVKAPFFKPELPWEMHCIEAATVIVRKGIWYM
                     FYAGAYNHERQQIGVAWSADGVNFRRFRDTPILPHGPKGSWNAWESGHPGVFEDDDGA
                     VYLFYQGKRSLNGDYLLSCKEVEFVD"
     gene            complement(1202..3142)
                     /locus_tag="PK576_08265"
     CDS             complement(1202..3142)
                     /locus_tag="PK576_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61624.1"
                     /translation="MKGFFLSRCRWLVPLVLAAEVWTAVSQEVRVSDFGFDAEDSTRF
                     LQAAIDSGAVRVVVDRQASPWVTEPLFCRSNQEIVFENGVEVVAKKGAFHGRNDCLFT
                     LKKVHDIKLTGLGDVVLRMHHDDYMSADYSRSEWRHVICILSSDRITLENLELRDSGG
                     DGLYVADARNGPASRDIVARRVISSGNARQGSSIISVEGLLYEDCVFANTRGRPPQAG
                     IDIEPNSGHNNVCGCIFRRCRFENNAGNGFQMSYPKEAKGRLPGSVRLEACVFRGNNY
                     GFSYGQNRKEPYYVKGEVSVTDCTFEGTRGPAIVISRKIGNSFKATVSNCRIENCCTG
                     SLNGSEIRINSLFGDFEPPSAIALKDIEIIQSTARDWIQFTNMPYGPHDKIDFTGNVL
                     VRSPAGVKRVAIDDAFRANAFPGFWNKGEPPRGKPMKEICTVVDSNPACSVKLTRLGL
                     RGTSRYVFYAAEAGPVIFIGSMKPASAVSPVQMRVVSAANGKEGRVLPMGQDGRLVVE
                     TAEPGLQTLTVLGDKEVFTLVESHVPVALEVAERPHALRVSSGDLFFYVPADRPFNVM
                     VCGESWGERVGAALYSPDGTEVWSEPGAIWWHGYRARGRVPEGLWRLNLRHPNKGVLQ
                     DYSVDMTGTNPYLFLSSEKYWK"
     gene            complement(3151..5772)
                     /locus_tag="PK576_08270"
     CDS             complement(3151..5772)
                     /locus_tag="PK576_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE61625.1"
                     /translation="MKKCMILFVIAAAFSVPAETWYIDAAAAKAHATAKSKPFDVPSV
                     WTNAAGDTASALLATDTLVVPDGVQLNYSGSMTAPAPLHLGVAGGGALAILHCSSGNL
                     TADIYWHNGRIQTQQYRASYGVTGTITVDGSEMGASHIWCYNKVQSTSTYNMSWNIWS
                     QLIGNAQQAIQFLYAPGTIKSEPVPIQQLPVVGNNVQWQGQFVVSAYANLILGHANAA
                     GSAETVRADAIVLGDHARLAVPVGVTPNAARGINITGSDVRFQARTYPHGFTDCTDYA
                     LNMPIAGTNGFTKTGAGRVVLGGAYTAGAIVVEEGTLEIVATATVTNGTQILVKNGAR
                     LITHISLAQVDVTVEEGGVFERVIDPFVVAFDSENVTTTPCVLSNEIVELEQIVPFRL
                     SESIPLPQHAAHKLAIFTYDGAETLMPPMFADTTPKTYGLPKGEITIESASGIQTVYL
                     NTRPVVVSRAKIEYASTNNINGSAETWSDGQTVHSNADYLLTHGYDSYHGSANVAFAG
                     DSLTLSETSFVSKASMNLFETASGNPLVFYPPFSSVQAHSGGRRHVYAGKIFLAGTYD
                     SEDAFTLETTYQDPARRTGTTTGSAELRADLTGEGVLFITARRLAGLNRLTGDNSGYR
                     GRILIQGNINDSDDGLFIEFGVAENLGGPLDAFHADALTLDQHAFLYPTTNVTLAADV
                     CRGMTVKDAGVLCPETTTLTTCWPLRLTGEFCKQGAGTLRLGGEISYGADGTGTNGTM
                     RVTDGTLSILSDAAVSGLAITFSNDVRLAVGPSLLRGLTVVPTVQDAVDGSAGHIRLA
                     IDAAAIPELWSGRIETVVATLPSGSADLTTMFGVPEHLRRYGVPDLVKRTVQIGDVTY
                     DQYVLTASCQKTVIIIR"
     gene            complement(5804..8191)
                     /locus_tag="PK576_08275"
     CDS             complement(5804..8191)
                     /locus_tag="PK576_08275"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOE61626.1"
                     /translation="MTQTVFTQSKSPAAIGILFLGCFAWAGNLTPLTVVESRVAQAEI
                     VCPDRSHAAERFAADELSRWIGEMTGTYVPVRNETTATAGRPKLYVGTDFAKDAFAAD
                     FKVIGKTDGFSVRTDGTNVYVFGTQPKGTLHAAYALLERNSDIIWPRPDPTVGVVHSL
                     TDSFVVVHADFREVPRTRVREWQWNYTGRGGAPNEALWGARNRQNQSGTPEGPLASCF
                     VSAGKGHGLQRYARPDTNFSVHPEWFPLIGGVRTRKGGQLCMTASGLADEIYASVTNE
                     LAQKYPDVSAKRIRVDYFNLTCADNHQVCECAACSSPFRCENGNVVPISDPCFRSAQY
                     YALMNQVARLLAKTHPRVTIGTYAYALTRYAPPFELAPNISVELCLTDYDERASLTDP
                     VRNLSARRHIDDWGDKCRGLWLRTYCGWSGRNLRAIEYAYAEACRYAAKRRFPLAQYS
                     AEIQRDEEVTGRHGAEIWDTSAISYWVISRLWWDPFQDVDVLRREFVMRTYREAAPQM
                     QAYFDAVRDAYAVDRMPATYRITCTAFMTQHYLYEPGLGPKLLGLLDEALAIARHPVS
                     REIIRRQRCRLQVNLDRAAALPTSRLAVRPLADGWGVAEVSRPFVGAAGIYGASSDKY
                     GGPAEVKTTFQAMHDDQALYLRVRCEAPDARTIYGASGNGSPEEQPDCDDALEIFLCR
                     PDTGAYWQWIMDPGAKGGPDIVFDAKGDDARWRGNWTRKVERDDFAWTMTMRVPFADM
                     AFEGCPDELRVNVIRARSPLNPPEEGKRKPRHFRSSWTGAGVHDSGGFGLLKFVK"
     gene            complement(8218..>8423)
                     /locus_tag="PK576_08280"
     CDS             complement(8218..>8423)
                     /locus_tag="PK576_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE61627.1"
                     /translation="PMGYLPFDFADKVEYAQFLHDGSELKIRRSSTQVHSQSGDLGAH
                     DSFVLPMKKPNVEIPVIEIFLKK"
BASE COUNT         1895 a         2655 c         2205 g         1668 t
ORIGIN      
        1 ggaatcacac atctgttcat ctcatctctc catgctgttc ccgaaaccaa tatggtttct
       61 cctagcatag gcggttccca aaacggcaga acctttttaa tccacaaact cgacttcctt
      121 acacgatagt agatagtcgc cattcaacga tctcttgccc tgatagaaga gatagacggc
      181 tccatcatca tcttcgaaaa caccgggatg accggactcc caggcattcc acgacccttt
      241 cggtccatgc gggagaatgg gtgtgtcgcg gaagcgcctg aagttcacgc catcggccga
      301 ccaggccacc ccgatttgct ggcgctcgtg gttgtaggct ccggcataga acatgtacca
      361 gatgcctttg cgtacaatca ccgtggctgc ttcgatgcag tgcatctccc aaggaagctc
      421 aggcttgaag aaaggcgcct tcaccgacaa ctccgtccac ttcgacgcgc catagtccga
      481 cccatacggc gcccaggcca cccccaactg ctggatgcgg gcattcggac gctcgcgcgt
      541 ggcgaacatc agcatcatcc gatcacccac gcggtagacc tctgcatcga ttgcgcgggc
      601 aatcgaccat tcgttctgcg gcacaaagac gggattctca ggcccatacg taaaatggat
      661 gccatccgtg ctggtggcat gccagagcac atctctcatc ctgccggatc caacaagcgg
      721 cggtccttgg gcgaaaagat ggatcttgcc gtcgaacttg cggacgcatg gcgccacacc
      781 acaacagaca gcacgcgcag gcccttgaaa gaggatatcg ctcacgcgca cccaatcaac
      841 cagattcgtg ctttcggcaa tcgcgccgca ccatccccac cgttctttcg gcacgtcggc
      901 gggacgactc tcggggggat acccacgcac gctgtagtac atcagataac gcccattgtg
      961 ccggatcacc gtcggatcct ttgcaaacgg acggtcagga atgcgcgacg tatcgccata
     1021 ccgcatctca ccgcccggaa gcggcttgaa cacgatatcg cggcacggtt gcgcaccgct
     1081 tcccgcacag gcctgcaatg tagcctgtgc gggaagccac ggtcccatca caaccaaggc
     1141 gaggatgagg aaaaggataa ggggaactgt ttttttcatc ttcgtctttc ttttgtttgc
     1201 attatttcca gtacttttcc gatgagagaa agagataagg attggtgccc gtcatatcca
     1261 ccgaataatc ttgcaatacc cccttgttcg gatgtcgcaa gttgagccgc cacaggccct
     1321 ccggcacgcg tccacgcgcc cgatatccgt gccaccaaat ggcccccggt tccgaccaca
     1381 cctccgttcc gtccggcgaa tacagtgccg cgcccacgcg ctctccccac gactcaccgc
     1441 acaccatgac gttaaaaggc cgatcggccg gcacatagaa aaacagatcc ccgcttgata
     1501 ccctaagcgc atgcggacgc tctgccactt cgagggcgac cggcacatgg gactcgacaa
     1561 gcgtgaaaac ctccttgtcg cccaaaaccg tcaatgtctg caagcccggc tcggcggttt
     1621 ccacgacgag tcgtccatcc tgccccatcg gcaggacacg cccttcctta ccattcgcgg
     1681 cagacaccac ccgcatctgc acaggagata ccgcagaggc cggtttcatg ctgccgatga
     1741 atatgaccgg cccagcctcg gccgcataga acacataacg cgaggtaccg cgaagcccca
     1801 aacgtgtgag tttgacggaa cacgcgggat tactgtccac caccgtgcag atctccttca
     1861 tcggttttcc acgcggtggt tcgcctttgt tccagaaacc ggggaacgca ttcgcgcgaa
     1921 acgcatcatc gatagccacg cgcttcacgc cagcaggcga acgcacgagg acgttacccg
     1981 tgaagtcaat cttgtcgtgc ggcccatagg gcatgtttgt gaactggatc cagtcgcgag
     2041 cagttgactg gatgatttcg atatccttca aggcaatggc ggaaggcggc tcaaaatcgc
     2101 caaaaagtga attgatgcga atttcgctgc cgttcagact ccccgtgcag caattctcaa
     2161 tacgacagtt cgacaccgtc gctttgaagg agttcccgat cttccggctg atcacaatcg
     2221 ctgggccgcg cgttccttca aacgtacagt ccgtcacgga cacctcgccc ttcacataat
     2281 agggttcctt acggttttga ccataggaaa aaccgtagtt gttgccgcga aacacacacg
     2341 cctccagtcg caccgatccc gggagacgtc cttttgcttc tttaggatag gacatctgaa
     2401 aaccgttgcc cgcgttgttt tcgaatctac agcgacggaa gatacaaccg cagacattgt
     2461 tgtgcccaga gttgggttcg atgtctatgc cggcttgggg ggggcgtccc cgtgtgttgg
     2521 cgaaaacaca gtcctcgtac agcagtcctt cgacgctaat gatcgacgat ccctgacggg
     2581 cattgccaga ggaaatcacg cgacgtgcaa caatatccct ggatgccggc ccgttacggg
     2641 catccgccac atagaggccg tcaccgccgg aatcacgcaa ctccagattt tcaagggtaa
     2701 tacgatccga actgaggata cagatgacgt gacgccattc actgcgcgaa tagtcggcac
     2761 tcatgtaatc gtcgtggtgc attcgcagca ccacatcgcc caaaccggtc aatttgatat
     2821 cgtgaacctt tttcagagtg aaaaggcagt cgttgcgtcc gtgaaacgca cctttcttgg
     2881 caacgacttc cacgccgttc tcgaagacga tctcctgatt cgaacggcag aagagcggct
     2941 cggtgaccca aggcgacgct tgacggtcca caactacccg gacggcaccg gagtcgatcg
     3001 ccgcctgcag aaagcgtgtc gaatcttcgg cgtcaaaacc gaaatccgaa acacgcacct
     3061 cctgcgaaac agccgtccag acttccgccg ccagaacaag cggcacgagc caacggcatc
     3121 gggacaggaa aaaacctttc acgccacgcc tcaccgaata ataatgaccg tcttctgaca
     3181 tgacgccgta aggacgtact gatcgtatgt cacgtcaccg atctgtaccg tccgcttgac
     3241 cagatccggc acaccatatc tccgcagatg ttccggcacg ccgaacatcg tcgtcagatc
     3301 ggcagatccc gacggaagcg tcgccacaac cgtctcaatc cggccggacc acagctccgg
     3361 aatcgccgcc gcatcaatcg caaggcgaat gtgccccgca gaaccgtcaa ccgcgtcctg
     3421 cacggtcggc acgacggtca gtccacgtaa taacgaaggc ccgacggcta gacggacgtc
     3481 attcgagaac gtgatggcga gacccgaaac ggctgcatcc gagagaatgc taagcgtacc
     3541 gtctgtcacg cgcatcgtcc cgtttgtccc cgtgccatcc gcaccatagg agatttcgcc
     3601 tcccaggcgg agcgtaccag cgccctgctt gcagaactcc cctgtcaaac gcagcggcca
     3661 acaggttgtc aacgtcgtcg tttccgggca caacacgcct gcatccttga cggtcatacc
     3721 acgacacacg tccgctgcca gcgtcacatt tgtcgttgga tagagaaacg catgctggtc
     3781 aagcgtcaag gcgtccgcat gaaacgcatc gagcggcccc ccgagattct cggcaacacc
     3841 gaactcgatg aaaagtccgt cgtccgagtc gttgatattc ccttgaatca ggatgcgtcc
     3901 ccgatagccg ctgttgtcgc ccgtgagacg gttcagtccc gccaggcgcc gcgccgtaat
     3961 gaacagaacc ccctctcccg tcagatcggc gcgcaactcg gctgatcccg ttgtggtacc
     4021 tgtacggcgc gccggatcct gatacgttgt ttcaagcgta aatgcatcct cggaatcata
     4081 cgtgccagcc agaaaaatct tgccggcata aacgtggcgg cggccgcccg aatgcgcctg
     4141 gacggacgaa aacggcggat aaaacacaag aggattgccg gatgccgtct cgaaaaggtt
     4201 catcgacgcc ttactgacaa agctcgtttc ggataacgtc agcgaatctc cggcaaatgc
     4261 cacgttagcc gaaccgtggt aagagtcata tccatgtgtc agcagatagt cggcgttcga
     4321 atgaacggtc tgcccgtccg accacgtttc tgccgaaccg ttgatgttgt tcgttgacgc
     4381 gtattcgatt ttcgcacggc tgacgacgac gggacgcgtg ttcagataca ccgtctggat
     4441 accgctggca gattctatgg tgatctcccc cttcggcagg ccgtacgtct ttggagtcgt
     4501 gtcggcaaac atcggaggca tcagcgtctc cgccccgtca taggtgaaga ttgccagctt
     4561 gtgcgctgcg tgttgtggca agggaatgga ttcggacagt ctgaagggca cgatctgctc
     4621 caactcgacg atctcattcg acaatacaca gggcgttgtc gtgacgtttt cactgtcaaa
     4681 ggcgacaaca aacggatcga tcacgcgctc aaacacgccg ccctcttcga ccgtcacatc
     4741 tacctgcgca agcgatatat gagtgatcag ccgtgcgcca ttcttgacta gaatttgcgt
     4801 cccattcgtg acagttgccg tcgcaacaat ttccaatgta ccctcctcga caacaatcgc
     4861 acccgccgta taggcaccgc ccaagacgac gcgcccggca cctgtcttcg tgaaaccgtt
     4921 cgtaccggca atcggcatat tcagtgcata gtccgtgcaa tccgtgaatc catgcggata
     4981 cgttcgcgcc tgaaacctga catccgatcc cgtgatgttg atgccgcgcg ccgcattcgg
     5041 cgtcaccccg accggcaccg ccagccgcgc gtggtcaccc agaacaatgg cgtcggctcg
     5101 caccgtctct gcggaaccgg ccgcattcgc atgtccgagg atcaagttcg cgtacgcgct
     5161 gacgacaaac tgaccctgcc attgcacatt gtttcccacc accggaagct gctgaatcgg
     5221 aaccggctcg gacttgatgg ttcccggcgc atacaggaac tgaatggcct gttgggcatt
     5281 gccaatcaac tggctccaga tgttccagga catgttgtac gtcgatgtgg actgaacttt
     5341 attgtaacac caaatgtgcg aggcccccat ctccgatccg tcaaccgtga tcgttcccgt
     5401 cacgccatag gacgccctat actgctgtgt ttgaattcgc ccgttatgcc agtaaatatc
     5461 cgcagtcaga ttgcccgaac tacaatgaag gatagccaaa gcgccaccac cggccacacc
     5521 gagatgcagc ggtgccggcg cagtcatgct gccgctatag ttcaattgga caccatccgg
     5581 cacgacaaga gtatccgtcg cgagcaatgc actcgccgta tctccggcgg cattcgtcca
     5641 gactgagggc acgtcaaacg gcttgctttt tgccgtcgcg tgcgctttcg ccgctgccgc
     5701 gtcgatatac catgtctcgg cgggaacaga aaaggccgcc gcgatcacaa acagaatcat
     5761 gcactttttc attctggtct cctgattggg tttttgggca tagttattta acaaacttca
     5821 acagtccgaa accacctgag tcgtgaacac cggcaccggt ccagcttgag cggaaatggc
     5881 ggggcttgcg ctttccttct tcaggcggat tcaagggact gcgggcgcga atcacgttga
     5941 cgcgcaactc atccggacat ccctcaaacg ccatgtccgc gaacggcacg cgcatcgtca
     6001 tcgtccacgc aaagtcgtct cgttcaacct tgcgtgtcca gttaccgcgc cacctggcat
     6061 catcaccttt cgcgtcaaag acaatgtcgg ggccaccctt ggccccggga tccataatcc
     6121 actgccagta ggcaccagta tcgggacggc acaggaagat ttcgagcgcg tcatcacagt
     6181 cgggctgctc ttccggcgaa ccgttgcccg acgcaccgta gatcgtgcgg gcatcaggcg
     6241 cctcgcagcg gacacgcaga tagagtgcct gatcatcgtg cattgcctga aaagtcgtct
     6301 tgacctcggc cgggccaccg tacttgtcgc tgcttgcgcc gtaaataccg gccgcgccga
     6361 caaaaggacg cgacacctca gctacgcccc acccgtcggc caggggacgc acggcaaggc
     6421 gactggtcgg cagggccgcc gcacgatcaa gattcacctg caaacggcaa cgctggcggc
     6481 ggatgatttc gcgagaaaca ggatgacgcg cgatggcaag cgcttcgtca agaagcccaa
     6541 gcagcttcgg ccccaatccc ggctcgtaaa gataatgttg cgtcataaag gccgtgcagg
     6601 tgatgcggta tgtcgcgggc atgcggtcga ccgcataggc gtcgcgcacg gcgtcaaaat
     6661 acgcctgcat ctgcggcgcc gcctcgcggt aggtgcgcat gacgaactcg cgccgcaaca
     6721 catcgacgtc ctgaaatgga tcccaccaaa gacggctaat gacccaatag gagatggccg
     6781 acgtgtccca gatctcggcc ccgtgacgtc ccgtcacctc ctcgtcacgc tggatctccg
     6841 cagaatactg cgccagcggg aaacgccgct tcgcggcata ccggcaggcc tcagcatagg
     6901 cgtactcaat ggcacgcaaa ttacgtcccg accaaccgca gtacgtacgc agccacaagc
     6961 cacggcactt gtcaccccaa tcgtcgatat gacgacgtgc ggaaagattg cgtacgggat
     7021 ccgtaagtga tgcgcgctca tcataatcgg tcagacaaag ctcgaccgag atatttggcg
     7081 cgagttcaaa cggcggcgca tagcgcgtga gcgcgtaggc ataggtaccg atcgtcacgc
     7141 gcggatgtgt cttcgccagg agacgcgcga cctgattcat gagcgcatag tactgcgccg
     7201 aacggaaaca aggatcggaa atcgggacaa cgttcccgtt ttcacaccgg aacgggctcg
     7261 aacaagccgc acattcgcaa acctgatggt tgtccgcaca cgtcaaattg aagtagtcaa
     7321 ctcggatccg ctttgctgaa acgtccgggt acttctgggc caattcgttc gtgacagaag
     7381 cgtagatttc gtcggcaagc ccgctcgccg tcatgcaaag ttgcccgcct ttcctcgtac
     7441 gcacaccgcc aatcaacgga aaccactccg gatgcacgga gaaattcgtg tcaggccgcg
     7501 cataacgctg caagccgtgc cccttgccgg cgctgacgaa gcagcttgcc agaggcccct
     7561 ccggcgtacc gctctgattc tgacgattcc gcgcccccca aagagcctcg tttggagcgc
     7621 caccacgtcc cgtgtagttc cattgccact cacgaaccct cgtacgcggc acttcgcgga
     7681 agtccgcatg tacgacgaca aacgaatccg tcaacgaatg aaccacgccc actgtcggat
     7741 cgggacgcgg ccagatgatg tcggaattcc gttcgagcaa cgcatacgcc gcatggagcg
     7801 ttcccttcgg ttgggtgccg aaaacataaa cgttcgtccc gtcggttcgc acggaaaagc
     7861 cgtctgtttt cccgatgacc ttaaaatcgg cggcgaaggc atccttcgcg aaatcggtgc
     7921 cgacgtagag cttgggcctg cccgctgtgg cagtggtttc gttccgaacc ggcacatagg
     7981 tcccggtcat ctcgccaatc caacggctca actcgtccgc cgcaaaccgt tcggccgcat
     8041 gagatctgtc cggacagacg atctcagcct gcgcgactcg cgattcaacg accgtcagcg
     8101 gcgtaagatt gcccgcccac gcaaaacacc ccaggaacag aattccgatt gctgcaggag
     8161 atttcgattg tgtgaaaacc gtctgggtca ttgcctcgtc cgccctctgc catcccatca
     8221 ctttttcaga aagatttcaa tgacgggtat ctcgacattc ggctttttca tcggcaggac
     8281 aaaactgtca tgcgcaccga gatcgccact ctgtgaatgg acttgcgtcg aactgcggcg
     8341 aatcttcagc tccgagccgt catgcagaaa ctgcgcgtat tcgactttgt ccgcgaagtc
     8401 gaacggcagg tagcccatcg gat
//
LOCUS       DAOSLE010000121         8361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_7958_length_8361_cov_8.094510, whole genome shotgun sequence.
ACCESSION   DAOSLE010000121 DAOSLE010000000
VERSION     DAOSLE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_8361_cov_8.094510"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="PK576_08285"
     CDS             6..161
                     /locus_tag="PK576_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HOE61628.1"
                     /translation="MNISVVDEQQILEASKNPEAYPDLIVRVTGFTAYFANLSPDFRK
                     LVLERIG"
     gene            396..2063
                     /locus_tag="PK576_08290"
     CDS             396..2063
                     /locus_tag="PK576_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61629.1"
                     /translation="MTFFRSVLTVSVFCLIAFGALAQPAPRPASDAPMPVIINMAADG
                     VVNFITGHFRKEGTNRAVAEAAIDKEVDQYVGTGVSHLFWNVNYQRVGYDSKAWPSYW
                     DVPDPETQVTEWPRGYYLLHKLGINDVFARLIPRSRAKGISPWISLRMNDHHYFNDFS
                     RVSPLLFNHPELRVNRTGLFNYTHPEVRDHYMKLIVEVLTRYDIDGLELDWMRTPNNF
                     PEQELDAGRELLTAFVREARRHAQAATQRRGHPVHLAVRVPATPEFAVAEGLDAVRWA
                     REGLVDMIVVSDFWGGCGDFPIERWRAQIGPDGAACKLIACTQCTYLCVPKGFQFVNN
                     IAAQRGFTASMADRGAEGIYLFNHFHAVTGIQRLRTPEGKSFEEGTLADLFRAAADRK
                     SAPAGRRIHPLAIHDGLPEKTDYRPALPAPLAVNNPSASFRLHIGPRPAATAACRLFI
                     GLYATNGVEAAQFDARVNGTACQPLPDLPRPTKDEFLKPKPDNWMTPPRRNLSELAGR
                     VLVFDVPTASLTRGYNAIGLTLTGGLPQSVTWLELVIDPEAGKPVAP"
     gene            2126..4135
                     /locus_tag="PK576_08295"
     CDS             2126..4135
                     /locus_tag="PK576_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61630.1"
                     /translation="MTKRILFALAFTVQSAWAIVNWVAKTDPAQNDFSNLDNWPAAIA
                     TDKAIQFYTLNPAAPELWMSEDATFARLVLGGTNIVFNLGSGRTLSVGQEGETKLNAA
                     KTFVRLTSGTINQTANRLVLGQDKNSSNCVFIADGPTTRYLNPQRGIHIGAASNAVSC
                     GMGVYNGAVVDGNFYIGNHAFSSGNWVEAAGTGTVVLATNNHFYVGDTAASSWNRLSL
                     SESAALQMMGANFCIGNNSGSDSNQAKILSGSSVTGQSETIVGNKGSENTLEAGNATL
                     KMSSLFTIGYYSGSNSVRLTNTFVQSNGIYLGRDPSSSGENTLEVIGGTVTNTGGGIR
                     VGIYGNRNTVVISNAYVCPAGNLSAGEYGSENSLSLYGDGTETRVAGLIFGGNAEAHK
                     NTLRLSGCVITNTGGFGNNGAENRIEIANGSKIWSGGHVGGTAAGVSNVLNLTGGSFF
                     SATTAFNFGNVSSFNTMIVDNSAFIASNVTFCLGYTEGTANSNTLAVINNGYLYAYRL
                     RIGDRGSYNTLIISNSTVHAGSNNCTIPYQNGISTNNQMVFIGTNCLLKSEADIYIRS
                     GTTLTFVIPRGGYAEPPIQTLADTAKNINIDPTTRIVLDLTEFARGGGGEQVLARTDN
                     KFSISETTLSLLNAQIAPQQCALEIVGKELILHTPHTGGTVISLW"
     gene            4176..6773
                     /locus_tag="PK576_08300"
     CDS             4176..6773
                     /locus_tag="PK576_08300"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOE61631.1"
                     /translation="MKKRIPFAAVLIFAGAAMQIRGAELAPRLIINEDNDHYFKQKSE
                     LMTEAALRAYIDTFADTQVTHFFMCPQGQRTSYRSRVHEAIWDPVNGVTPTNIWCVNC
                     KLLFDKGIDPYHVWIEQCRKRGISPWITMRMNDVHFVNIPDYFRNTTFWRERRDLWRN
                     PDTTTNNWMECAFDYSKEEAYAYHMAVVRELFERYDFDGFETDWLRFPYHLTPGKAFA
                     QRDVLTRFMREVRALADLWAKKRGHPIRISARVASDPDVAAHLGTDAVAWARDGLIEL
                     LVASCFFSTADFDIPIERWHARLGDLKDRVPVIPGTDNGFSPYPGSPRQSLDIHLYRG
                     WAEACRYRGANAFYLFNLVYFSFDRPPFRPIIEKGFPAPILAQEPRRHTITYRDALPS
                     EGGFESGAQLPQTTEAPCRFVIQTGQKPQYGKVSVITGLAAHPQVSNTVLTVSLNGTR
                     ATNRTVEPQVRRYGKLTAYAVRHTFPLSALRDGSNTVDVASAPQPRQRIEWVEIEITP
                     ATPLEHVGPPLPRHAVTNRAFTGISSLAVAPNGRLWVTWYAGHTPNEDNNNYVVLSTS
                     GDHGRTWKEVLIADPDGSGPRRTFDPEVWLAPDGKLRWFFTDRKNGLPATDLLWMIVL
                     DDPGSESTAWHPPAHIADGVMMCKPIVLSTGEWALPVCTWYTENSSKMVVSTDSGKTW
                     SVRGGASMPKEDRLFDEHMFVERKDRSIWCLTRTKSGIREAVSTDRGATWSPLEPSPI
                     QHPSARFFITRLLSGNLLLVKHGPIGKKTGRSHLTAFVSRDDGKTWEGGLMLDERNGV
                     SYPDGQQTADGTIYITHDFDRTGKRHILFSTFREEDALAGKPVSGAVRLRQLVSEGSG
                     GKERPQATK"
     gene            7029..>8361
                     /locus_tag="PK576_08305"
     CDS             7029..>8361
                     /locus_tag="PK576_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61632.1"
                     /translation="MVRETTGIAAATIILCGLTSLHAQPRDTPLPGRGAALFVQGFFE
                     QDTFTEGARLFPDRTYTVAEAPAWLRGLTFLRANIDGNLTVTAKQAGVLTVITADPAD
                     PCATHSQCARLEKLGFVWIKAPATFQLFGKAAYDISRVYQKQVAQDETFRFPKWTVFA
                     GFSAVTGPPPPFDLQPGRGERLYNGIELPTNWPPRTVNTADWAPMAVPYLDVPPELIH
                     IDVGRQLFVDDFLIATSTLQRVFGMPEKYSGNPVLRPETELELNGIRNAAAVPKGGGL
                     WWDPHEHLFKLWYEAGWIHTICYATSTNGLDWVRPELDVVPETNQVLPPDLTPDSWTV
                     VPDWEATDPLQRYKMFMRGPGGNMSGVSMTSADGIHWVNRVITGNTGDRSTMFYNPFR
                     RKWIYSLRSGWRGRSRDYRESDNFLRGASWSAAETVRWLAVDEKDPKDPKIQ"
BASE COUNT         1752 a         2625 c         2348 g         1636 t
ORIGIN      
        1 cgctcatgaa catcagcgtc gttgacgagc aacagattct ggaagcgagc aaaaaccccg
       61 aggcctatcc cgatctgatt gtgcgcgtga ccggcttcac cgcctacttc gccaacctct
      121 cgcccgactt ccgcaaactc gtgctcgaac gaatcggata gccgtttgag gaacagcatg
      181 ccaaagatga actcctcata cggctctccc gaaaaacaaa aacccgccag atttgagcat
      241 gtccctttcc ccgtcgccgg gaaatcgaaa aatctccgct caccctcttg acgccatcta
      301 accgaacggt taaactggta tacaacttta aaaaacttgt gtttttaacc cgcccgcccg
      361 tccgggaaga gccgggcgtc gtcttggagg agaggatgac tttttttcgt tccgttctga
      421 ctgtttcggt cttctgtctc atcgccttcg gtgccctggc gcaacccgcc ccccgccccg
      481 catccgacgc ccccatgccc gtgatcatca acatggcggc agacggggtg gtcaacttca
      541 taaccggcca ctttcgtaaa gagggaacca accgcgccgt ggccgaggcg gcgatcgaca
      601 aagaggtgga ccagtatgtg ggaactgggg tctcccacct tttctggaat gtcaactacc
      661 agcgtgtcgg gtatgacagc aaagcctggc cgagctactg ggacgttccg gacccggaaa
      721 cccaagtcac cgaatggccc cgcgggtact acctgcttca caagttgggc atcaacgacg
      781 ttttcgcgcg cctgattccc cgttcccgcg ccaagggcat ctcgccctgg atctcgctgc
      841 gcatgaatga tcaccactat ttcaacgatt tctcccgcgt cagccccctg ctcttcaacc
      901 atcccgagtt gcgcgtcaac cgcaccgggc tcttcaacta tacccatccc gaggtgcgcg
      961 atcactacat gaagctgatc gtcgaggtgc tgacccgtta cgacatcgat gggttggaac
     1021 tggactggat gcggaccccc aacaacttcc cggagcagga gctggacgcg gggcgtgaac
     1081 tgctcaccgc attcgtccgc gaggcgcgcc gccatgcgca ggccgcaacg caacgccggg
     1141 gacacccggt ccacctcgcg gtgcgcgttc cggccacgcc ggagttcgct gtcgccgagg
     1201 gtctggacgc ggtccgttgg gcccgggagg gtcttgtgga catgatcgtg gtcagcgact
     1261 tctggggcgg ttgcggcgac tttccgattg aacgctggcg cgcgcagatc ggtcccgacg
     1321 gcgctgcctg caagctgatc gcctgcaccc aatgcaccta cctgtgtgtg cctaaggggt
     1381 tccagtttgt caacaatatc gcagcgcaac gcgggtttac cgcatcgatg gctgaccgcg
     1441 gtgcggaagg catctatctc tttaaccatt tccatgccgt caccggcatc cagcgtctgc
     1501 gcaccccgga aggcaaatct tttgaggaag gcacgctggc cgatctcttt cgcgccgcgg
     1561 ccgaccgcaa gagcgccccc gccggccgcc gtatccatcc tcttgcaatc cacgatggcc
     1621 tgcccgaaaa aaccgattac cgtcccgcgt tgcctgcccc gctcgccgta aacaacccgt
     1681 ccgcatcctt ccggctgcac atcggtccac gtcctgccgc aaccgcggcc tgccgcctct
     1741 tcatcgggtt gtacgccacc aacggtgtcg aagccgcaca gttcgacgcg cgcgtcaacg
     1801 gaaccgcatg ccaaccactc cccgacctcc cccggcccac gaaagacgaa tttctaaaac
     1861 ccaaacccga caactggatg acgccccccc gccgcaacct cagcgaactg gccggacgtg
     1921 tcttggtttt cgacgtgccg accgcatccc tgacgcgtgg atacaacgcc atcgggctca
     1981 ccctgaccgg cggcctcccc caaagcgtca cctggctgga actcgtcatc gatcctgaag
     2041 cgggaaaacc cgtcgcaccc tgattcgagg tgttcgtcaa aatgtgaacg tgctacaata
     2101 ctgcaaataa tgaaaggagt gggtcatgac aaagcgcatt ctgttcgccc ttgccttcac
     2161 cgtgcaaagc gcctgggcga tcgtgaactg ggtcgcgaaa accgaccccg cacaaaacga
     2221 tttctcaaac ctggataact ggcccgcagc cattgccacg gacaaggcga ttcaattcta
     2281 cacgctgaat ccggccgcgc cggaactgtg gatgagcgag gacgccacct tcgcccggtt
     2341 ggttctgggc ggcacgaaca tcgtcttcaa tctgggatcc ggccgcacgc tttcggtggg
     2401 tcaggagggc gagaccaaac tcaacgccgc caaaacattc gtccggctga ccagcggaac
     2461 gatcaaccaa accgccaacc gactcgtcct cggacaagat aaaaactctt ccaactgcgt
     2521 gtttatcgct gacggcccga ccacccgata cctaaatccg caacgcggaa ttcacatcgg
     2581 ggccgcatcg aacgccgtaa gctgcgggat gggcgtttac aacggcgctg ttgtggacgg
     2641 caatttctac atcggcaacc atgcgttctc ttcaggcaac tgggttgagg ctgccggcac
     2701 cggaacggtt gtcctggcga caaacaacca tttctacgtc ggcgacacag cggccagcag
     2761 ttggaaccgg ctctcccttt ccgaatccgc cgcactccaa atgatgggcg ctaatttctg
     2821 tatagggaac aacagcggct ctgattccaa tcaggcgaag atcctgtccg gctcgtccgt
     2881 gacaggccaa tccgagacga tcgtcggcaa caagggttcc gaaaacacgc tggaggccgg
     2941 gaacgccacg ctcaaaatgt cctcgctgtt cacgatcggt tattatagcg gatccaattc
     3001 tgtccggctc acgaacacgt tcgtgcaatc caacggaatc tatctgggcc gcgatccgtc
     3061 gagcagcggc gagaatacgc tggaggtcat cggcggcacg gtcacgaaca ctggcggcgg
     3121 catccgggtc ggcatatatg gcaaccggaa cacggtcgtc atcagcaatg cgtacgtgtg
     3181 tccggccgga aatctttctg ccggcgaata cggttccgag aacagcttgt cgctgtatgg
     3241 cgacgggacc gagacccgtg tcgccggttt gattttcggc ggaaacgcgg aagcgcacaa
     3301 aaacaccctg agactatccg gatgcgtgat caccaacacg ggagggttcg gcaacaacgg
     3361 cgcggaaaac cggatcgaga tcgcgaacgg ttcgaaaatc tggtccggcg gccacgtcgg
     3421 tggcacagcg gcaggcgtct cgaatgtgct gaacctgacg ggcggttcgt ttttctctgc
     3481 gaccaccgct ttcaatttcg gtaatgtctc ctccttcaac accatgatcg tggacaacag
     3541 cgcgttcatc gcgagtaacg tcaccttttg tctggggtac accgaaggca ccgccaacag
     3601 taacacgctt gccgtcatca acaacggata tctgtatgcc tatcgcctcc gcatcggaga
     3661 cagaggatca tacaataccc tgatcatcag caacagcacg gttcacgccg ggagtaacaa
     3721 ctgcacgatc ccttaccaaa acggaatctc caccaacaac cagatggtgt tcatcggcac
     3781 caactgcctt ttaaaatccg aagcggacat ttacatccgc agcggaacca cgcttacgtt
     3841 cgtgatcccg cgcggcggat acgcggagcc cccgattcaa acgcttgccg atacggcaaa
     3901 aaacatcaac atcgatccca cgacccggat cgttctcgac ctcaccgagt tcgcgcgcgg
     3961 cggtggcggg gaacaagtcc tggcgcgtac ggacaacaaa ttcagtatca gtgaaaccac
     4021 cctgtcgttg ctcaatgcgc aaatcgcacc gcaacagtgc gcactggaga tcgtcggcaa
     4081 ggaactgatc ctgcacacac cccatacggg cggcacggtc atttcgctat ggtgagggcc
     4141 aacgtcccgg ccgaagaacg gaaacgagaa cctttatgaa aaaacgaata ccctttgcgg
     4201 ctgtcctcat ttttgcgggc gccgccatgc agatcagggg cgccgagttg gcgccccgcc
     4261 tgattatcaa cgaggacaac gaccactact tcaaacagaa gtctgaattg atgaccgagg
     4321 cggcgctacg cgcctatatc gacaccttcg ctgacacaca ggtgacgcat ttcttcatgt
     4381 gcccccaagg tcagcgcacc agttaccgct cccgcgtgca cgaggcgatc tgggacccgg
     4441 tcaacggtgt cacgccgacc aacatctggt gcgtcaactg taaactcctt ttcgacaaag
     4501 ggatcgaccc ttaccatgtc tggatcgaac agtgccgcaa gcgcgggatt tcaccctgga
     4561 tcaccatgcg tatgaacgat gtgcactttg tgaacatccc ggactacttc cgcaacacga
     4621 ctttctggcg cgagcgccgc gacctctggc gcaacccgga caccaccacg aacaactgga
     4681 tggagtgtgc gttcgactac tcgaaggagg aggcctacgc ctatcacatg gctgtcgtgc
     4741 gggaactgtt cgaacgctat gatttcgacg gctttgaaac cgactggctg cgcttcccct
     4801 atcacctgac gcccggcaag gcgttcgcgc aacgcgatgt gctgacccgc tttatgcgcg
     4861 aggtgcgcgc cttggcagac ctctgggcga aaaaacgcgg acacccgatc cgcatttcag
     4921 cgcgcgtggc ctcggatccg gatgtggcgg cccatctcgg caccgacgcg gtggcgtggg
     4981 cgcgcgacgg attgatcgaa ctgctcgtcg cctcctgctt tttctccact gcggacttcg
     5041 acatcccgat cgaacgctgg cacgcgcgcc tcggcgacct caaggatcgc gttccggtca
     5101 ttcccggcac tgacaacgga ttctccccct accccgggtc accgcgtcag tcattggata
     5161 tccatctcta tcgcggttgg gcggaagcct gccgttaccg tggcgcgaac gctttctatc
     5221 ttttcaatct ggtgtacttt tcctttgacc gccccccttt ccgtccgatc atcgaaaagg
     5281 ggtttccggc cccaatcctg gcacaagagc ctcgtcggca cacgatcacc taccgcgacg
     5341 cgctaccctc ggaaggcggg tttgagtctg gcgcgcaact gccgcaaacc acggaggcgc
     5401 cctgtcgttt tgtgattcag accggccaga aaccccaata cggaaaagtc tccgtgatca
     5461 ccggactggc tgcccatccg caagtctcga acaccgtgct gacggtttcg ctcaatggaa
     5521 cgcgcgccac caatcggacg gtcgaacccc aggtgcggcg ttatggcaag ctcacggcgt
     5581 atgcggtgcg tcacaccttc ccgctctccg ccctgcggga cggctccaac acggtggacg
     5641 ttgcgtccgc cccacagccc cggcaacgga tcgaatgggt tgaaatcgag atcacacccg
     5701 ccacgccgct ggagcatgtg ggcccgccgc tgcccagaca cgcggtgacc aaccgggcct
     5761 tcaccggtat ctccagtctg gcggtcgcac ccaacggccg tctgtgggtc acttggtacg
     5821 cgggacacac tcccaacgaa gacaacaaca actacgttgt gctcagtacc agcggcgacc
     5881 atggccggac ctggaaggaa gtgttgattg ccgaccccga tggatccggt ccgcgccgca
     5941 ccttcgatcc cgaagtttgg ctcgcaccgg acggaaaact gcgctggttc ttcacggacc
     6001 gcaagaacgg attaccggca accgatttgc tctggatgat cgtgctcgat gatcccggtt
     6061 ccgagtctac ggcctggcat ccgcccgcgc atatcgctga cggcgtcatg atgtgcaagc
     6121 cgattgtgct ctctacaggc gagtgggcgt taccggtctg cacctggtat acggagaaca
     6181 gttcgaagat ggtggtctcc accgacagcg gcaagacgtg gtccgtgcgc ggcggtgcga
     6241 gtatgcccaa ggaggaccgc ctctttgacg aacacatgtt tgtggaacgc aaagatcgct
     6301 cgatctggtg cctgacgcgt accaagtccg gtatccgcga ggcggtctcg accgaccgcg
     6361 gtgcgacgtg gtcgccgctg gagccgtcgc cgatccagca cccttcggcg cgcttcttca
     6421 tcacgcggct gctttccggc aacctgttgc tggtgaagca tggtccgatc ggaaagaaga
     6481 ccggccgctc gcatctgacc gcctttgttt ccagagacga cggcaagacg tgggaaggcg
     6541 gcctgatgct tgatgagcgc aacggggtct cctatccgga cggtcaacag accgcagatg
     6601 gcacgattta catcacccat gacttcgacc gcaccggtaa acgccacatt ctcttctcca
     6661 ccttccgcga ggaggatgcg ctggccggca agccggtttc cggcgcggtg cgtctgcgtc
     6721 agcttgtgag cgagggatcg gggggaaaag agcgccccca agccactaaa tgataccatg
     6781 aaacacatga aacttcatga aaagcggggt gtggttagtt cattagttcg ttagttcatt
     6841 agttcattag tgcgttagtt cgttagtgcg ttagtttggt ttcatcgaaa tcggtatcgg
     6901 tatcgggaga aagtgagtaa cgactgatga aaggagtcct tgatttcatg aagtttcatg
     6961 tgtttcatgg ttaagagacg tatgcaattt tcaagtttca tgtgtttcat ggtttgggga
     7021 gagaaagaat ggtgagagaa acaacgggaa tcgcggctgc aacgatcatc ctgtgcgggc
     7081 tgacatcgct tcacgcacag ccacgcgaca cacctttgcc ggggcgcggt gccgcgctct
     7141 tcgtgcaggg gttttttgag caggacacgt tcaccgaggg tgcgcggctc tttccggacc
     7201 gcacttacac ggtggctgaa gcacccgcct ggttgcgcgg gttgaccttt ctgcgcgcaa
     7261 acattgatgg caatcttacc gtgacggcca agcaggccgg tgtgctgacc gtgatcacgg
     7321 ctgatcccgc cgatccgtgc gccacgcatt cgcaatgcgc gcgtctggag aagctggggt
     7381 ttgtctggat caaagcaccg gcgacctttc agcttttcgg caaagcggct tacgacatca
     7441 gtcgcgtcta tcagaaacag gtcgcgcagg acgagacgtt ccgtttcccc aagtggacgg
     7501 tgttcgccgg tttctctgcg gtgaccggac caccgccgcc gttcgacctt cagcctgggc
     7561 gcggcgagcg gctttacaac ggcatcgaac ttccgaccaa ctggccgccg cgcaccgtga
     7621 acaccgcaga ctgggcaccg atggccgtgc cgtatttgga cgtgccgccg gagttgatcc
     7681 atatcgatgt ggggcgacag ttgttcgtgg atgattttct gatcgcaacc agcacgttgc
     7741 agcgcgtctt cggcatgcct gagaagtata gcggcaaccc agtactgcgt ccggagaccg
     7801 aactcgagtt gaacggcatc cggaacgcag cggccgtgcc gaagggcggc gggctctggt
     7861 gggatcccca cgagcatctt ttcaaactct ggtacgaagc cggctggatc cacaccattt
     7921 gttacgctac cagcaccaac gggttggatt gggttcgtcc cgaactggac gtggtgccgg
     7981 aaaccaacca agtactgccg ccggacctca cccccgactc ctggaccgtg gtgcccgatt
     8041 gggaggcgac cgatccgctg cagcgctaca aaatgttcat gcgcggaccg ggtggaaaca
     8101 tgtcgggcgt gagcatgacc tcagcggacg gcatccactg ggtgaaccgg gtgattaccg
     8161 gcaacaccgg cgaccgcagt accatgttct acaatccctt ccgccgaaaa tggatctaca
     8221 gcctgcggag tggctggcgc ggacggtcac gcgattacag ggagagcgac aacttcctgc
     8281 ggggtgcttc ttggagcgcg gcggagaccg tgcgttggtt ggccgtggat gagaaggatc
     8341 ccaaggatcc gaaaatccag c
//
LOCUS       DAOSLE010000122         8285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8053_length_8285_cov_8.168894, whole genome shotgun sequence.
ACCESSION   DAOSLE010000122 DAOSLE010000000
VERSION     DAOSLE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_8285_cov_8.168894"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="fucP"
                     /locus_tag="PK576_08310"
     CDS             <1..964
                     /gene="fucP"
                     /locus_tag="PK576_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOE61633.1"
                     /translation="ETATRRLNLAQAFNPMGSLLGMLVASKLVLRNLQAKDFFAGITA
                     KLSAQGVPESGMAEAIKTYLAEPANQAAYAAMKTHDITTIRDPYVILGLVVAVFFFIF
                     LFSKIPDQRSAEQIHPIETAKRLFRNRNWVGGVIAQTFYVAAQITCWTYIIQYADRNC
                     GFSFERAQDWNIAAMLIFLSSRFICTFLMKYLNASLLLTLLAIGGIVLTTLTILLQNL
                     AGLVCLVGVSACMSLMFPTIYGIALDGLGEDAKLGSAGLIFAIVGGALMPMGAGWFID
                     QGSFNLGFITLTGESASFVLPLTSFIVIAIFGWRTFRSLRPKPA"
     gene            980..2356
                     /locus_tag="PK576_08315"
     CDS             980..2356
                     /locus_tag="PK576_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE61634.1"
                     /translation="MPPKTPATRRKKKQLVKTFVLDTNVLLHSPVALAVFDDNHVVLP
                     MAVLEELDKFKAQSSELGRNARESIRKLDALCQNGKLAEGVKLPGGGTLRVLATPEIE
                     KAGLEEHTPDNALIRLAYWLERQGERVIIVSMDINVRVKANAVGLDAEDFEHEKVNFD
                     ELYCGYAEASVPRAVIERLYNGQMLTKAPVRCHPNMFLYLSATEEPDMKVIARVFPDG
                     TLAEIDLSIDTVYGIQSRNIEQRMALELLMDPAVTLVTLMGQAGTGKTLLALAAGLHL
                     VQREHLYDRILVSRPIVPLGKDIGYLPGDKEEKLSHWMEPIFDNLDYLLRKTQPNQLP
                     PGAKKQPKMLTPQDRINQLLENDTLELEALTYIRGRSISRQFILIDEAQNLTPHEVKT
                     VISRAGEGSKIILTGDPYQIDNPYLDASSNGLTYAAERLKELPLHGHVTLHQSERSQL
                     AAAAAQYL"
     gene            2385..2885
                     /locus_tag="PK576_08320"
     CDS             2385..2885
                     /locus_tag="PK576_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOE61635.1"
                     /translation="MKSIPGTTPVQKGAVFLATGFGIGLIAPFAPGTFGSLPGVVLAL
                     WLTRQVLPLQILACLGLALLAVPICGVAETVLGKKDDGRICADEWMLFPICTIGLPLL
                     QHLWLVPFCFVVARLCDILKPPPARSLQALHGGVGIVIDDFFASLYALALNHVGYRLI
                     LPFLTS"
     gene            2932..4005
                     /locus_tag="PK576_08325"
     CDS             2932..4005
                     /locus_tag="PK576_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOE61636.1"
                     /translation="MMNDSTRTLLKELLSLPTPTGFELAGMRLLAARLRDAAVPGVRF
                     DVHGNLHACLNPDAPLRVMIEGHGDEIGFMVQHIDDDGFLYMCPLGGVTVPLLAGERL
                     VIQGRNGPVYGVFGVRPPHLLSAKDRENVAPKEIREIAVDIGARNQAEALELVDLGSP
                     AVVASGWLDLANNRAACRGFDNRIGAFIVTEAMRRLAREQLKVAVHMVASVQEEVGLV
                     GGTTAAYDIRPHIGLCVDVTFATDAQKEDRKIAGDIRLGAGPALAIGPTYHTALNARV
                     VEAAEKARIPLQRQVRNRGTGTCAWAMRLSRSGAAVVQLSIPLRYMHSPVEVLSLDDA
                     ARTIDLLCATLLDLPADLDLCPS"
     gene            4009..5190
                     /locus_tag="PK576_08330"
     CDS             4009..5190
                     /locus_tag="PK576_08330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE61637.1"
                     /translation="MPYPIVYLLDRNAASETFIRREIDLLQRRNWPVLIRYVTSGSNV
                     LKFALGACPRTGRGRFVRAACRRLLAEGFRSPVTACRILKRLPQAASLAALAAETECR
                     LIHAQFAGLTAELAALASQTLGIPWTCAVHARDVFTVSPRLLGRRLRSAAAVAACSRA
                     AAEAVTAAGYPPQQIAVIHHGLPLNDFAFDSIQPDGVILFVGRLEAKKGVDTLLHACA
                     LLRGRGVPFTCVIAGEGPRRKDLTRLSDRLGLAENVVFIGWQSPEETRYHIMDASILV
                     LPSRRLSNGDRDGIANVLLEAMALGTPVLTTTAGAAPEVLTDQVNGLLVPPDDPVALA
                     DALAQALTSKSQLLRLAQAGRATIEQHFDGSKTISEIEAFFQRAVTPPSPEPPVPTIS
                     A"
     gene            complement(5144..6100)
                     /locus_tag="PK576_08335"
     CDS             complement(5144..6100)
                     /locus_tag="PK576_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOE61638.1"
                     /translation="MRFALSTNWCALRHNSGEALAEEIRSLGFDALELGYHTTEELAQ
                     GIERCVRDGRAVVDSVHAYCPVPIGAPHGYPELYLLASRDEDERAMAAILLGRTREFA
                     ERMGARAVVLHAGRVFLRTLFGERGTRALLEARLEEGDQETGRSAALLTKALRLRTRR
                     VRAVFDGFCLSLERLLPAFEKAGMSLCLENLPSLEAFPDETEMMMLKRRFDTPALAYW
                     HDLGHGQVRENLGWIRHVESARLLLPFTRGTHIHDVAPLMEDHLVPGAGQIDFGAFAF
                     YGTESVLKVFEPAAEAKGEALRDALARLRRIWSEPAVPETGA"
     gene            complement(6129..6707)
                     /gene="folE"
                     /locus_tag="PK576_08340"
     CDS             complement(6129..6707)
                     /gene="folE"
                     /locus_tag="PK576_08340"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOE61639.1"
                     /translation="MKRKQIDQARVTELVRKLLVELGEDPEREGLRGTPKRMADALAF
                     LTGGYRQTPRQILNGAVFQAHLNHMIIVRDIEVYSLCEHHLLPFYGRCHVGYIAKDKV
                     LGVSKIARIVDCFARRLQIQERLTEEVAQAILTEAGAEGVGVVMECRHLCMMMRGVEK
                     QNSVMTTSAVLGSFRTDPAKRQEFMTLIHRQV"
     gene            7222..8106
                     /locus_tag="PK576_08345"
     CDS             7222..8106
                     /locus_tag="PK576_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOE61640.1"
                     /translation="MMAKFSKGVVLFAFALAQLHVVAVDVNWTGGADDGNKWSTGGNW
                     QGGQTPTTDDVAVFNVASATTINVDTEDAIAKQVKIAAASAAVTFGGDGIDCCGVKDT
                     VIRNSFIHTQDDCIAVKWSAENLTVSNCIFVTDEANIVRMGYECGVHAKFENVVFRDI
                     DALHLAMAKRTAEKYWCNGCLLLQASHEAVMRDILFENIRLKDFEDGRDELFVAKTMS
                     IKSIAGDNGPWTTPLAGHINGVTLRNITGPRQFTRADIKLIEKDQDHKIENVKFIQPP
                     SVRETEVSSTVMRVPRRR"
BASE COUNT         1592 a         2770 c         2364 g         1559 t
ORIGIN      
        1 cgaaacagcc acgcggcgtc tgaatctggc gcaggccttc aacccgatgg gctcgctcct
       61 cggcatgctg gtcgccagca agctggtgct gcgcaacctg caagccaaag attttttcgc
      121 tggcatcacc gcgaagctga gtgcgcaagg cgtccccgaa agcggtatgg ccgaggccat
      181 caaaacctac ctcgccgagc ccgccaacca ggccgcctat gccgcgatga aaacccacga
      241 catcaccacc atccgcgacc cctacgtcat cttggggctc gtggtggcgg tcttcttctt
      301 tatcttcctg tttagcaaga tcccggacca gcgctccgcc gaacagattc atcccatcga
      361 aacagccaag cgcctgttcc gcaaccgcaa ctgggtcggc ggcgtgatcg cccagacctt
      421 ctacgtcgcg gcccagatca cctgctggac ctatatcatc cagtacgccg acagaaactg
      481 cggcttcagc tttgaacgcg ctcaggactg gaacatcgcc gccatgctca tctttctcag
      541 cagccgcttc atctgcacct tcctcatgaa atacctgaac gccagcctgc tgctcacgct
      601 gctggccatc gggggtattg tgctgaccac gctgaccatc ctgctgcaga atctggccgg
      661 cctggtctgt ctggtcggag tctcggcctg catgtcgctg atgttcccca ccatctacgg
      721 catcgcgctg gacggactcg gagaggacgc gaaactcgga tccgccggac tgatcttcgc
      781 cattgtcggc ggggcgctga tgcccatggg cgcgggctgg ttcatcgacc aaggctcctt
      841 caatctcggc ttcatcacgt tgaccggcga aagcgcctca ttcgtgttgc cgctgaccag
      901 tttcatcgtg atcgccattt tcggctggcg caccttccgc tccctcaggc ccaaaccagc
      961 gtaatccgcg ctctttgtca tgcctcctaa aactcctgcc acacgccgca aaaaaaagca
     1021 gctcgtcaaa acctttgtgc tcgacaccaa cgtgctgctt cactcaccgg tagccctcgc
     1081 cgtgttcgat gacaaccatg tggtgctgcc gatggccgtg ctggaggagt tggacaaatt
     1141 caaggcccag agttccgagc tgggccgcaa cgcgcgcgag tcgatccgca agctggacgc
     1201 gctgtgccag aacggcaaac tcgccgaggg cgtcaaactc cccggcggcg gcacgctgcg
     1261 cgtgctggcg acccccgaga tcgaaaaagc cggcctggag gagcacaccc cggacaacgc
     1321 cctgatccgg ctcgcctact ggctggaacg acagggcgaa cgggttatca tcgtctcgat
     1381 ggacatcaac gtccgcgtca aggccaacgc cgtcgggctc gatgccgaag acttcgagca
     1441 tgagaaggtc aacttcgatg agttgtactg cggatacgcc gaagcgtctg tgccgcgcgc
     1501 cgtgatcgaa cgcctctaca acggccagat gctgacgaag gcccccgtcc gttgccaccc
     1561 gaatatgttc ctgtatctct ccgcaacgga agagcccgac atgaaagtca tcgcccgcgt
     1621 gtttccggac ggcacgctcg cggagatcga cctgtccatc gacacggtgt acggcatcca
     1681 atcgcgcaac atcgagcagc gcatggcgct cgaactgctg atggaccctg cggtgaccct
     1741 cgtcaccctc atggggcagg cgggtaccgg caagacgctg ctggccctcg cggcaggcct
     1801 tcatctggtt cagcgtgaac acctctacga ccgcatcctc gtctcgcgcc ctattgtgcc
     1861 gctcgggaaa gacatcggtt accttccggg tgacaaggag gagaaactct cgcactggat
     1921 ggagccgatt tttgataatc tcgattacct gttgcgcaaa acccagccca accaacttcc
     1981 gcccggtgcg aaaaaacagc ccaagatgct gacgccgcag gaccgcatca accaacttct
     2041 cgaaaacgac accctcgagc tggaagcgct cacctacatc cggggccgct ccatctcccg
     2101 ccagttcatc ctcatcgacg aggcacagaa cctcacgccg cacgaggtca agaccgtgat
     2161 cagccgcgcg ggcgagggct ccaaaatcat tttgaccggc gacccgtacc agattgacaa
     2221 cccgtatctg gacgcgtcga gcaacggact gacctatgcg gccgaacggc tcaaagagct
     2281 gccgctccac ggtcatgtca cgctgcatca gagtgaacgc agtcagcttg cggccgcagc
     2341 cgcccaatat ctctgacgcc catcgcggcg tggagcggag gaagatgaaa agcatacccg
     2401 gcacaacccc ggttcagaaa ggggccgttt tcctggccac cggtttcgga atcggcctga
     2461 tcgcgccgtt cgcgcccggc acattcggct ccctgccggg tgtcgtgctc gccctctggc
     2521 tcacgcgtca agttctgccc ttgcagattt tggcatgcct cggcctcgcg ctgctggcgg
     2581 ttccgatctg cggtgtggcg gaaaccgtgc tgggcaaaaa agacgacggg cggatctgcg
     2641 cggacgagtg gatgctcttc ccgatctgca cgatcggcct gccgctgttg cagcaccttt
     2701 ggctggtacc gttctgtttc gtcgtggcgc ggctctgcga catcctcaag ccgccgccgg
     2761 cccgctcgct gcaagcgctg cacggcggcg tgggaatcgt gatcgacgat tttttcgcct
     2821 cgctgtatgc gttggcgctg aaccatgtcg gataccggct gattttgcct tttctgacct
     2881 cctgacccaa tgtgagcccc caacccaacc taacctgtgt aaggtgttct gatgatgaat
     2941 gattcaaccc gtaccctgct gaaagaactt ctctccctgc ccacacccac cggcttcgag
     3001 ttggccggca tgcggctgct ggccgcgcgg ctgcgcgacg ccgcagtgcc gggcgtccgt
     3061 ttcgacgtgc acggcaatct gcatgcgtgc ctgaaccccg atgcgccgct gcgggtcatg
     3121 atcgaggggc acggtgatga aatcggtttc atggtccagc atatcgacga cgacgggttc
     3181 ctgtacatgt gcccgctcgg cggggtgacc gttccgttgc tcgcgggcga acgccttgtg
     3241 attcagggcc ggaacgggcc ggtttacggc gtgttcggcg tacggccgcc ccatctgctc
     3301 tccgccaagg atcgcgaaaa cgtcgcgccg aaggagatcc gggagatcgc cgtggacatc
     3361 ggggcacgga atcaggccga agcgcttgaa ctcgtggacc tcggcagtcc ggccgtggtg
     3421 gcctcaggct ggctcgattt ggctaacaac cgcgccgcct gccgtggttt cgacaaccgc
     3481 atcggcgcgt tcatcgtgac ggaggcgatg cgtcggctgg cccgtgaaca actcaaggtg
     3541 gcggtacaca tggtcgccag cgtgcaggag gaggtcggac tggtcggcgg aaccactgcg
     3601 gcctatgaca tccgtccgca catcggcctc tgtgtggacg tgactttcgc cacggacgcg
     3661 cagaaagagg accgcaaaat cgctggcgac atccgtctcg gggccggacc ggcgctcgcc
     3721 attggtccga cctaccatac cgcgctcaac gcgcgcgtcg tcgaggccgc agaaaaggcg
     3781 cgcatcccgc tccagcggca ggtgcgcaac cgtggaacgg gaacctgcgc ctgggccatg
     3841 cgcctgtcgc gttccggtgc ggcggtggtg caactcagca tcccgctccg ttatatgcac
     3901 agtccagtcg aagtcctcag tctggatgac gccgcgcgca ccatcgatct gctgtgcgcc
     3961 accctgctgg acctgcccgc cgatcttgac ctctgcccaa gttgacgtat gccctatccg
     4021 attgtctacc ttctggatcg gaacgccgcg agcgagacgt tcatccggcg cgagatcgat
     4081 ctgctgcaac gccggaactg gcccgtgctc atacggtatg tgacatccgg atccaacgtg
     4141 ctgaaattcg ctctgggtgc ctgcccgcga accggccgcg gccggttcgt ccgtgccgcg
     4201 tgccgacgcc tccttgcgga agggttccgc tcgcccgtca ccgcctgccg cattctcaag
     4261 cgcctgcctc aggccgcatc cctcgccgca ctggcggctg aaacggagtg ccgcctgatt
     4321 cacgcccagt tcgccggtct caccgctgaa ctcgccgcac tcgcctccca aacgctcggg
     4381 atcccctgga cctgcgccgt ccacgcccgg gatgtcttta ccgtctcgcc gcgcctgctg
     4441 ggtcgccgcc tgcgctcggc cgccgccgtg gcggcctgtt cccgcgctgc ggccgaagcc
     4501 gtcaccgcag ccggttaccc gccgcaacaa atcgcggtga tccatcacgg actgcctctg
     4561 aacgacttcg cgttcgactc catccagccc gacggggtca tcctgttcgt tggccgtctg
     4621 gaggcgaaaa aaggcgtgga cacgctgctt cacgcctgcg cactgctccg cggacgcggc
     4681 gtgccgttca cctgtgtcat tgcgggcgag ggaccccgcc gcaaagacct cacccgcctc
     4741 agcgaccgtt tgggactcgc ggaaaacgtt gtcttcatcg gctggcaatc gccggaggag
     4801 acccgctacc acatcatgga tgcgtcgatc ctcgtgttgc cttcgcggcg tttgagtaac
     4861 ggggaccgcg acggcatcgc gaatgtgctg cttgaggcca tggccctcgg cacaccggtc
     4921 ctgaccacca ccgctggcgc ggcccccgaa gtgctcaccg atcaggtgaa cggtctgctg
     4981 gtgccgccgg acgacccggt ggcgctggct gacgcgctgg ctcaggcttt gacctcgaaa
     5041 agccagctgc tgcgcctcgc gcaggctggc cgcgccacca tcgaacagca tttcgacggg
     5101 tcaaaaacga tctcggaaat cgaggcgttc ttccaacgcg cggttacgcc cccgtctccg
     5161 gaaccgccgg ttccgaccat atccgcctga gtcgggcgag cgcgtcgcgc aacgcttccc
     5221 ccttggcctc cgccgcaggt tcaaacacct tcaaaaccga ttccgtcccg taaaaggcga
     5281 acgcgccgaa gtcgatctgg cctgctcccg gaaccagatg atcctccatc agcggggcca
     5341 catcgtgaat gtgtgtgccg cgcgtgaacg gcagcagtag acgcgccgac tcgacatgac
     5401 ggatccatcc gagattttcg cgtacctgac cgtgaccgag gtcatgccag tacgcgagtg
     5461 cgggggtatc gaagcgccgt ttcagcatca tcatctccgt ctcatccggg aacgcctcca
     5521 aggaaggcag gttttccaga cagagtgaca tgccggcttt ctcgaacgcg ggcagcaacc
     5581 gctccagcga gaggcagaaa ccgtcaaaca ccgcgcggac ccgccgggtc cgtaaccgta
     5641 acgccttcgt caagagcgcg gccgaacgtc ccgtttcctg atccccttcc tccaaacgcg
     5701 cctcaagcag tgcccgcgtg ccccgctccc caaaaagcgt tcgcaggaaa acgcggccag
     5761 cgtgcaacac gacagcccgt gcccccatcc gttcggcgaa ctcgcgcgtg cggcccagca
     5821 ggatcgcggc catggcccgc tcatcctcgt cccgcgacgc caacaggtat aactcgggat
     5881 aaccgtgcgg ggcgccgatc ggcaccggac agtacgcatg cacactgtcc accacggcgc
     5941 gcccgtcccg cacgcaacgc tcgattccct gggccagttc ctccgtggtg tgatacccga
     6001 gttcgagcgc gtcgaaaccg agactgcgga tctcctccgc cagcgcctcg cccgagttgt
     6061 gccgcagcgc gcaccagttt gtcgacaacg cgaaacgcat cgtacctcgc tcctcttgca
     6121 ccgcagcctc agacctgccg gtgaatcagc gtcatgaact cctgccgctt ggccggatcg
     6181 gtccggaagg aacccagcac cgccgaggtg gtcatcaccg aattctgctt ctccaccccc
     6241 cgcatcatca tgcagagatg gcggcactcc ataaccacgc cgaccccctc cgcgccggct
     6301 tccgtcagaa tcgcctgcgc gacctcctcg gtcagccgct cctgaatctg gagccgacgg
     6361 gcaaaacagt ccacgatccg ggcaatcttg ctgaccccca gcactttgtc cttcgcaatg
     6421 tatccgacat gacaacgccc gtagaacggc agcagatgat gctcacagag actgtagacc
     6481 tcgatgtcac gcacgatgat catgtgattc aagtgcgcct gaaacaccgc accgttcaga
     6541 atctgacggg gcgtctgccg atatccgccg gtgaggaacg ccaacgcgtc cgccatgcgt
     6601 ttgggcgtac cccgcaaccc ctcccgctcc ggatcctcgc cgagttccac caacaatttg
     6661 cggaccagct cggtgacccg tgcctgatca atctgttttc gcttcatgcc gccactgtac
     6721 cccagccctt tcctgaactc aagccgtgag tacaggcacg ccttcatgaa catggcccct
     6781 aaccatgaaa cttcatgaaa agcttggcta cggtcaaatc cgttcatcag acttgcggaa
     6841 tgccgcatcg gaatcgggag cgggagtgtt ttcgtgtaca ggtgtgaccc gtcggcttga
     6901 aacgctacgt tgaagcttga aacctgaaac caaagcgtgt tttgagttca gtgtcccggg
     6961 agggtcaaac gtctgcttga ctggcggcgg tcacgccgcc cacaaggaca ggagcacatt
     7021 ttgtgcgatg acaagagccg tgctgtacgc gggtccgcga tgcctcttcc cggccagttt
     7081 ccccttgccc ataaccctcc cgtatgccag actaatatca ggtttatcac ctgaaggact
     7141 cgcgcccccg ctgatcaatg acccatgttg cactgcactg gctggcgggc gcggatattt
     7201 caccgcgaac aaggaataat catgatggct aagttctcga aaggcgtcgt tctgttcgca
     7261 tttgcactgg cgcaactgca tgtcgttgcg gtggacgtaa actggaccgg cggcgcggat
     7321 gacggcaaca agtggtctac cggcgggaac tggcagggcg gccagacacc aaccacggac
     7381 gatgtcgcgg ttttcaacgt cgcctcggca acaacgatca acgtcgatac cgaggatgcg
     7441 atcgccaagc aggtgaaaat tgcagcggcc tcggcagcgg tcactttcgg cggtgacggc
     7501 atcgattgct gcggggtgaa ggatacggtc atccgcaact cgttcatcca cactcaggac
     7561 gactgcatcg cagtcaagtg gtccgccgag aaccttactg tctccaactg tattttcgtg
     7621 acggacgaag cgaatatcgt ccggatgggc tacgagtgcg gcgttcacgc gaagttcgag
     7681 aacgtcgttt tccgggatat cgacgcgttg catctcgcga tggcgaagcg cacggcggag
     7741 aagtactggt gcaacggctg ccttctattg caggcatcgc acgaagcggt catgcgcgat
     7801 atccttttcg agaatatccg gcttaaggat ttcgaggacg gccgggatga gctcttcgtc
     7861 gcgaagacaa tgtctataaa atccatcgcc ggtgacaacg ggccctggac gacaccactc
     7921 gccgggcaca tcaacggtgt cacgctccgt aacatcacag gaccgcggca atttacgcgc
     7981 gcggatatca agctcatcga gaaggatcaa gaccacaaga tcgagaacgt gaagttcatc
     8041 caaccgccgt ccgtccgcga gaccgaggtt tcttcaacgg tcatgcgcgt accgcgacga
     8101 agatgagtag agacacatcc aaacctcgcg ctttcgcgag gcagactcaa cagcacgata
     8161 acgttggcag gaggaagatg aaagtcatgc ctacaatcgg tttcacgctg ttcatatcgt
     8221 tgttcttcgc cgttgggttc gccatcctcg gctacggcat acgcagcctg gtcctgtcca
     8281 acaag
//
LOCUS       DAOSLE010000123         8208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8136_length_8208_cov_6.444008, whole genome shotgun sequence.
ACCESSION   DAOSLE010000123 DAOSLE010000000
VERSION     DAOSLE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8136_length_8208_cov_6.444008"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..854
                     /locus_tag="PK576_08350"
     CDS             144..854
                     /locus_tag="PK576_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61641.1"
                     /translation="MRLFLMMTGWGIAIFCLLPFTQGANDQFPYQLMAFFTVLPLFVH
                     LAKYPLPKRVAGCLVVLNLFMLVPLIAVHSTDRTVARAYAVYPLDPCKHNLEMSWNVH
                     LGLLLGDNFQDNPVIRETALRIYREGAMRKDSFAGMNYLLYVSALYQYGRFEEGVKLL
                     RDLLSRNPQIVSRFYAVRPNFIYFNRQRLWDDIERVFPIRDENTLRQLRTVRRQIEAQ
                     ARRERYSVRRPAYAVTEL"
     gene            complement(869..1903)
                     /locus_tag="PK576_08355"
     CDS             complement(869..1903)
                     /locus_tag="PK576_08355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE61642.1"
                     /translation="MLLPHPSDALRRLSILIVTYDGDELLRACLDSIQRACGDMPGTV
                     VVDNAASETTRELVAAFPRTRYVRAETNLGFAGGNNLGFPFCEGEYILLLNNDTVIHD
                     EPFTALIRYCDANPQVGVAQGKIRFGGNIDLLDGCGSFLTAIGIQYAYGFLVPDAPAF
                     QKPYPVFAASGAFFLIRRSVIAQAGGYLFYDHFRSYYEEVDFCHRVWLSGSEVHFVPT
                     PVIDHLHSQTAGRFRRTDILRQYYQNIWFSLLTCLGGYGRLRILPVFTAFFAGNALAH
                     LLAGHRELFRAHLDTVRKLWIMRDQIRSARKTIRGFRVRSDRELFRIILKSPPLSYYW
                     RSIRSNAFSS"
     gene            2048..4159
                     /locus_tag="PK576_08360"
     CDS             2048..4159
                     /locus_tag="PK576_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61643.1"
                     /translation="MNGKKVFAATVMMAGMALQGLAMLRCETQPDLTVHVRPGGDDAG
                     PGTQAHPLGTLAKARDMVRTARQQRALTTGAEIVLHGGRYVLNDTLMLSAEDGGTSAA
                     PIRYRAARGEQPVLAGSTAIPASAFLPLADPERLARLEAPARERVVAVDLKALGFPAM
                     PKPRDVFRMPMCVPELFCNGRRMTLACWPNEGFAHIAKILDMGTQRGTGSVSDSADPK
                     KKRTQENRGGCFVYEGDRPARWRVEEGVWLHGFWCFDWYDDVIRIGAIDAEKKEIRLQ
                     AAHEYGVRQGNPSPRRWRAVNVFDELDAPGEYMVDLQAGRLYFYPPEPLAGSRITLAF
                     RGEPLVRMRDLSHVIFEGLTFEETQGDALRVTGCSHVVVSRCVFRNHRMRAIVVEGGV
                     KNRIASCDIHDTGVGGVILDGGDRKTLSPAGHVLEDTHIWRYSQHLQTYASALTLKGV
                     GNVARHNLLHDAPHMAVAISGNDHLFEYNVVSNVVMASDDAGALYKGRNPSCRGNVIR
                     YNLWSEIGSPRGHGTAAIYFDDGDGGDTVYGNLFYRCGDPGKGSFGTVFSHGGHGNVA
                     ENNVFIECKRALGSSPWNDKRWKQFIDAPLWQERLLREVDITSGTYTGRYPELIGFMD
                     PQPGALRENIAIRNLFVACDGITKGNWNTNAVYVATAEDPGFVNYAKRDFRLKRNAAV
                     WKRMPEFKPLPLEKMGLRRKR"
     gene            complement(4198..7734)
                     /gene="dnaE"
                     /locus_tag="PK576_08365"
     CDS             complement(4198..7734)
                     /gene="dnaE"
                     /locus_tag="PK576_08365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE61644.1"
                     /translation="MNKPFVHLHLHTKYSLLDGACHIEPLVERAKELGMNALAMTDHG
                     VMYGAIDFYNACRDAGIKPILGCEIYILAKAHRGTRDHTIPYHHLVLLARNREGYINL
                     ARINTISHLEGFYYKPRIDKETLRAHAGGLIGLSACLKGEVNALLAERHLDEAEKVAL
                     EYRDIFGPDNFFLEMQDHGLPEQKRVNEGVRELCRRTGLKAVVTNDVHYLHQNHAEAH
                     EVMLCIQTQTVMSDPKRMRYASDEFYFKSREEMEKRFPDDREAFDLTQEIADRCDVQL
                     TFSNGKAETLHFPAFDVPAGFEDARQYLVHLGKQGLKRHYGFEYDDPRDATQKMLVER
                     FDYEVGVIENVGFINYFLVVADFVNWSRNNGVPVGPGRGSGAGSLLAYSLGITQIDPI
                     RFDLIFERFLNPERVSPPDFDIDFCQMRRERTIEYVKRKYGKERVAQIVTFGQLGAKT
                     VIRDIARVLEIPLYQADKFSKMIPEDPKMTLAKAKTENPQFAEACAQDPLLQQIMRHA
                     VVLEGLYRNAGVHAAGVVIGDKPLIDLIPLGRDKDGSPVTQYAKEPVEACGLLKMDFL
                     GLKTLTVLQESVELVRMTRGIEVDLENLPLDDPKTFDLFRRADTVGVFQLESDGMRRT
                     LVDLQPTRIEEIIAILALYRPGPMDMISLFVKRKKGEEKITYDHPLLEPILKETYGIM
                     VYQEQVQRAAQVLAGYSLGKADMLRRAMGKKKAGEMAKERTGFIAGCGKTNGIPEEQA
                     GQIFDNIEKFAGYGFNKAHAAAYGMITYQTAYMKAHYPVEFMCAQISSEIGNFDKLPG
                     FVAEAEAMGIRVLPPDVNHSLDRFAPENGNIRYGLAGIKSVGTGAAEAIIQARAQTAF
                     TGLTDFYERVDPSSVNKRVLEALIRCGAMDAFGMHRARLFFNIDFALARANEKLREKA
                     SGQRSLFEMLDADEANAVCTDDLPDYPAWSEKERLTGERELLGMYMTGHPLTRYRRII
                     QALQTFSAARGEETPDKQDVRIAGMAASVVTRISKKTKEPWAIITLDDGETNIEVLVY
                     TRAFRKYEGACRQDQPVLVCGKLSKKEDQIKIIAEEVYPLIEAPRQFVEKVVVGIRTD
                     DGQTSGRIKALRSLVDRHPGITPILICLLYPDSKRMLIRTNEAMTIDPSPEFIAEAEG
                     ILGPDHFRFTARKAIYREAHDDSRWGWNGTDR"
BASE COUNT         1613 a         2209 c         2640 g         1746 t
ORIGIN      
        1 tggcaaaagg gccttatgcg ggtcgcgaag tgtttccgca ccggcccctt tcagggaata
       61 cgctgattca ccttgctaat catgtgctga ttccccttgc gcctcttgca gtgagtttgt
      121 tctgtttttc caatcgggga tacatgcggt tgttcctgat gatgacgggg tggggtatcg
      181 cgattttttg cctgttaccc ttcacgcagg gggcgaatga ccagtttcct tatcaattga
      241 tggcgttttt cacggtgctt ccgctgtttg tccacctcgc caagtatccg ctaccgaaac
      301 gtgtggccgg gtgtctggtg gtgttgaatc tgttcatgct tgtgcccctg atcgcggtac
      361 acagcacaga ccggacggtc gcgcgcgcgt atgccgtcta tccgctcgat ccgtgcaaac
      421 ataatctgga gatgtcctgg aatgtgcatt tggggctgtt gctgggggac aattttcagg
      481 acaatccggt cattcgggag acggccctgc ggatttatcg cgagggggcg atgcggaaag
      541 attcgttcgc gggcatgaat tatctcttgt atgtttcggc gctttaccag tacggtcggt
      601 ttgaggaggg cgtaaagttg ttgcgggatc ttttgtcgcg caatccgcag atcgtctcgc
      661 ggttttatgc ggtccggccc aatttcatct acttcaaccg gcagcgctta tgggatgaca
      721 tcgagagggt tttcccgatt cgtgacgaga acaccctgcg acaactgcgc acggtacggc
      781 ggcagatcga agcgcaggcg cggcgcgagc ggtattctgt ccggcggccg gcgtatgccg
      841 tgacggaact ctgaaggcgg tccggggatc aggatgagaa ggcgttggat cggatggaac
      901 gccagtaata gctcaatggg ggggacttga ggatgatccg gaacagttcg cggtcggaac
      961 gtacgcggaa cccgcggatt gttttgcgcg ccgagcggat ctgatcccgc atgatccaga
     1021 gtttgcgcac ggtatccaga tgggcgcgga acagttcgcg gtgaccggcc agcagatgcg
     1081 ccagcgcgtt tcccgcaaag aaagcggtga aaaccgggag aatccggagg cgtccgtatc
     1141 cgcccaggca ggtcagcagc gaaaaccaaa tgttctggta atactggcgc aggatatcgg
     1201 ttctccggaa acgcccggct gtctggctgt gcagatggtc gatgacaggc gtgggaacga
     1261 aatgcacctc ggaaccgctc agccagacgc ggtgacagaa atcgacctcc tcataatacg
     1321 aacggaaatg atcgtaaaaa agatatccgc ctgcttgggc gatcacagag cggcggatca
     1381 ggaaaaaggc accgctggcg gcgaagaccg ggtacggttt ttggaaggcg ggcgcgtcag
     1441 ggaccagaaa cccgtaagcg tactggatgc cgattgccgt cagaaaggag ccgcatccgt
     1501 ccaacaggtc aatgttgccg ccgaatcgta tcttgccttg cgcaacgccg acctgcggat
     1561 tcgcgtcaca gtaccggatt agcgcggtga acggttcgtc atgaatgacc gtgtcgttgt
     1621 tcagcaacag gatgtattca ccctcgcaaa acggaaaccc cagattgttg ccgcctgcga
     1681 aaccgagatt ggtttcggct cggacgtagc gcgtacgggg gaaggccgca accagttcgc
     1741 gggtcgtctc actggcggcg ttgtcgacaa ccacggttcc cggcatgtcg ccacaggccc
     1801 gttgaatgga gtccagacac gcccgcagca attcgtctcc gtcgtaggtg acgatcagga
     1861 tggataaacg acgcaaagcg tccgaggggt gcggcaacaa catgcccgga atataatcga
     1921 aacggggaga ggaaagcaag cgcggacaaa agaggcggag gcgggaaagg gcttggggtt
     1981 gcgttcctct gatggagatg tcatactgca aacgggatgc acgaaaggaa aacaaaggag
     2041 aaatacggtg aacgggaaaa aggtgtttgc ggcaacggtg atgatggccg ggatggcgtt
     2101 gcaggggttg gcgatgctgc ggtgcgagac ccaacccgat ttgacggtgc atgtgcgtcc
     2161 cggcggtgat gatgctggtc cagggacaca ggcgcatccg ctgggcaccc tcgccaaggc
     2221 gcgggatatg gtgcggaccg cccgtcagca acgtgcgctg accactggtg ccgagattgt
     2281 tttgcacggt ggccgttatg tgttaaacga cacgttgatg ctgtcggcgg aggatggcgg
     2341 cacatccgca gcaccaatcc gataccgtgc ggcccgcggc gagcagccgg ttctggccgg
     2401 atcgacagcg attccggcga gcgctttcct ccctctcgca gatccggagc ggctcgcgcg
     2461 gctggaagca cccgcccgcg aaagggtcgt ggccgtggat ctgaaggcgc tagggtttcc
     2521 cgcgatgccg aaaccccgcg atgtgttccg gatgccgatg tgtgtgccgg agctcttctg
     2581 caacggtcgg cggatgacgc tggcctgttg gccgaatgag ggcttcgcgc atatcgctaa
     2641 aattctcgac atggggacgc aacgcgggac cggttcggtg tcggattcgg ctgaccccaa
     2701 gaaaaagagg acgcaggaaa atcgcggcgg atgctttgtc tatgaggggg accggccggc
     2761 gcgctggagg gtggaggagg gggtatggct gcacggcttc tggtgttttg actggtatga
     2821 cgacgtgatc cggattggcg ccatcgatgc ggagaaaaag gagattcggt tgcaggccgc
     2881 gcatgagtac ggggtgcggc agggtaaccc ctcgccgcgt cgctggcgtg cggtgaatgt
     2941 gtttgacgag ttggacgcgc cgggcgaata catggtggat ttgcaggcgg ggcggctcta
     3001 tttctatccg ccggaaccgc ttgcgggttc tcggatcacg ctcgcgtttc gcggggagcc
     3061 actggtacgg atgcgggatc tttcgcatgt gatcttcgag gggctgacct tcgaagagac
     3121 gcagggtgat gcgttgcgtg tgaccggctg tagtcatgtg gtcgtcagtc ggtgtgtgtt
     3181 ccgcaatcac cgcatgcggg cgatcgtggt ggaggggggt gtgaaaaacc ggatcgcctc
     3241 ctgtgacatt cacgacaccg gtgtcggcgg ggtgattttg gatggggggg atcggaaaac
     3301 gctttcacct gcgggacatg tgttggagga cacccacatc tggcggtatt cccagcacct
     3361 ccagacctac gcttccgcct tgactctgaa aggggtgggg aacgtggcgc ggcacaacct
     3421 gcttcatgat gcgccgcata tggctgtcgc gatcagcggg aacgaccacc tcttcgagta
     3481 caacgtggtg agcaatgtgg tcatggcctc tgatgatgcg ggcgctctgt acaaggggcg
     3541 caacccttct tgccgcggta atgtgatccg ctacaatctg tggagcgaga tcggcagtcc
     3601 gcgcggtcac ggcacggcgg cgatctactt tgatgacggg gatggcgggg acaccgttta
     3661 tggaaacctg ttttaccgtt gcggcgatcc cggcaaaggg tcgttcggca cggtcttctc
     3721 ccacggggga cacgggaatg tggccgagaa caacgtgttc atcgagtgca agcgcgcgct
     3781 gggctcgtca ccgtggaacg acaagcgttg gaaacagttc atcgacgcac cgctatggca
     3841 ggaacgtctg ctgcgggagg tggacatcac cagcggaacc tataccggac gttatccgga
     3901 actgatcggg ttcatggatc cgcaacccgg tgcgctgcgc gagaatatcg cgatccggaa
     3961 tctgtttgtg gcctgcgacg gcatcacgaa gggcaattgg aacaccaacg ccgtatatgt
     4021 cgcgacggcg gaagatccgg gattcgtcaa ctatgccaag cgcgatttcc gcctgaaacg
     4081 taacgccgcg gtttggaaac gcatgccgga gttcaagcca ttgccgttgg aaaagatggg
     4141 cttgcggcgt aagcggtaaa ggcgcagtcc ggctgtgccg gatcccaatc gaaaccctta
     4201 tctgtcggtg ccgttccagc cccagcggga atcgtcatgc gcttcccggt aaatggcttt
     4261 gcgagcggtg aaacggaagt gatccgggcc gaggatgccc tccgcctcgg cgatgaactc
     4321 gggtgaaggg tcaatggtca tcgcttcatt ggtgcggatc agcatgcgtt tgctgtccgg
     4381 atagaggagg cagatcagga tgggggtgat gccgggatga cggtccacca aactgcgcag
     4441 cgccttgatc cgtcccgaag tctggccgtc atccgtacgg atgccgacca ccactttctc
     4501 cacaaactgg cgcggcgcct cgatcaacgg gtaaacctct tcggcgatga ttttgatctg
     4561 atcctctttt ttggatagtt tgccgcagac gagcaccggc tgatcctgcc ggcacgctcc
     4621 ctcatatttc cggaacgcgc gcgtatacac cagcacctcg atgttcgtct cgccatcatc
     4681 aagcgtgatg atggcccaag gctctttggt ttttttgctg atccgggtga cgacggaggc
     4741 ggccatgcca gcgatgcgga catcctgttt gtccggggtc tcctcgccgc gggctgccga
     4801 aaacgtctgg agcgcctgaa tgatgcggcg gtaccgggtg agggggtgtc cggtcatgta
     4861 catgccgagc agctcgcgtt ctccggtgag gcgttccttt tccgaccagg ccgggtaatc
     4921 gggcaggtca tcggtgcaga cggcgttggc ctcgtcggca tccagcattt caaagaggct
     4981 tctctggcca ctggccttct cgcgcagctt ttcattcgcg cgggccaaag cgaaatcgat
     5041 gttgaaaaaa aggcgggcgc ggtgcatgcc gaacgcgtcc atggcaccgc aacggatcag
     5101 ggcttccagc acgcgtttgt tgaccgagga gggatcgacg cgctcataaa aatcggtcag
     5161 cccggtgaat gcggtttgcg cccgggcctg aataatcgcc tctgcggcac cggtgccaac
     5221 gcttttgatg ccggcgagcc cgtagcggat attcccgttt tcgggcgcga accggtccaa
     5281 actgtgattc acatccggcg gcagcacgcg gatgcccatc gcctcggctt cggcgacaaa
     5341 tccgggcagt ttatcgaagt tgccgatttc cgaggagatc tgggcgcaca tgaactcgac
     5401 cggataatgc gctttcatgt aggcagtctg atacgtgatc atgccgtacg cggcggcgtg
     5461 ggctttgtta aacccgtatc ccgcgaactt ctcgatgttg tcgaagatct ggccggcctg
     5521 ctcctcgggg atgccgttcg tctttccgca tccggcgatg aatcccgtgc gttccttggc
     5581 catctccccg gcttttttct tgcccatggc ccggcgcagc atatcggctt tgccgagcga
     5641 gtatccggcc agaacctgag ccgcgcgttg cacttgttcc tgatagacca tgatgccata
     5701 ggtttctttc aggatcggct ccagcagagg atggtcgtac gtgatcttct cctcaccctt
     5761 tttgcgcttc acgaacaggg agatcatgtc catggggccc ggacggtaga gcgcgaggat
     5821 ggcgatgatt tcctcgatgc gggtgggctg caggtccacg agggtgcgcc gcatgccgtc
     5881 ggattcaagc tggaacacgc cgacggtgtc ggcgcgccgg aaaaggtcga aagttttcgg
     5941 atcgtccagc ggcaggtttt cgagatccac ttcgatgccc cgcgtcatgc gtaccagttc
     6001 cacggattct tgcagcacgg tcagggtctt gaggccaaga aagtccattt tcaacagacc
     6061 gcacgcctcg accggctctt tcgcatactg cgtgacgggt gaaccgtcct tatcccgccc
     6121 gagcggaatc agatcgatca gcggtttgtc gccgatcacc acgccggccg catgcacccc
     6181 cgcgttacgg tacaggcctt cgagcaccac ggcgtgccgc atgatctgtt gcagcagcgg
     6241 gtcctgggcg catgcctcgg cgaattgcgg attttcggtc ttggctttgg ccaacgtcat
     6301 tttcgggtct tcgggaatca tcttgctgaa tttgtccgcc tgataaagcg ggatttccag
     6361 cacgcgcgca atgtcgcgga tcacggtttt ggcaccaagc tgaccgaagg tgacgatctg
     6421 cgcgacacgc tccttgccgt atttccgttt gacgtattcg attgtccgtt cccggcgcat
     6481 ttgacagaaa tcgatatcga aatcgggcgg cgacacgcgc tctggattga ggaaacgctc
     6541 gaaaatgagg tcgaagcgga tcggatcgat ctgggtgatg ccgagcgaat aggcgagcag
     6601 cgacccggcg cccgaaccgc ggccggggcc gacaggaacc ccgttgttgc gcgaccagtt
     6661 gacgaagtcc gccaccacga ggaagtagtt gatgaagccc acgttttcga tcacgccgac
     6721 ctcatagtca aaccgctcga ccagcatctt ctgggtcgcg tcgcgcgggt cgtcgtattc
     6781 gaacccgtaa tgacgcttta acccctgctt gccgagatgg accagatact gccgggcgtc
     6841 ttcgaatccg gctggcacgt cgaacgcggg aaagtgcagg gtctcggcct tgccgttgga
     6901 aaacgtcaac tgcacgtcac agcggtccgc gatctcctgg gtgaggtcaa aagcctcgcg
     6961 gtcatcgggg aagcgcttct ccatctcttc ccgcgacttg aaataaaact cgtctgaggc
     7021 gtaccgcatc cgtttcgggt cgctcatcac ggtttgggtc tggatgcaga gcattacttc
     7081 gtgcgcttca gcatggttct gatggaggta gtggacatca ttggtcacca ccgctttcag
     7141 gccggtgcgc cggcagagtt cgcgcacgcc ttcgttgacc cgcttctgtt ccggcagccc
     7201 gtggtcctgc atctcaagga aaaaattgtc cggcccgaag atgtcccggt actcgagcgc
     7261 gactttttcc gcctcatcga gatgccgttc cgcaaggaga gcattgactt cgcctttgag
     7321 acatgcggag agaccgatca gaccaccggc gtgtgcgcgg agggtttctt tgtcgatgcg
     7381 cggtttgtag taaaagcctt cgaggtggga gatggtgttg atgcgcgcca gattgatgta
     7441 cccctccctg ttacgcgcca acaggaccag atggtggtat gggatcgtgt gatcgcgcgt
     7501 gccgcggtgg gccttggcca gaatgtagat ttcacatcca aggatcggtt taatccccgc
     7561 gtcgcggcag gcattgtaaa aatcgatggc gccgtacatg actccgtggt cggtcatggc
     7621 caacgcgttc atgcccaatt ctttggcacg ttcgaccagc ggttcgatgt ggcaggctcc
     7681 gtccagcagg ctatatttgg tgtggagatg gaggtggaca aaaggcttgt tcatatgagg
     7741 ggctccgccg gccggcgcgg ggaaagtggg aaacggaaag ggggcgcgcc ggaaccccgg
     7801 atcccctttg ctgttcagga tagcaaaacg ctgggaaggc gggaaggctg aaaaactttt
     7861 cgttcagggc gcgcgttgag gcgggcaaag gaagagacaa gccagggttt ttcgacagct
     7921 tcgctttggc ggggaggctg tgagggtgtt aggctgtgag ggtgtgaggg tgtgaggcgg
     7981 tgagggtgtg agggtgtgag gctgtatcga tcccgatagc gatagcgatc ccgaccccga
     8041 tttagatgaa accatgaaac acatgaaact tcatgaaaat caggaattcc ccctggcgtt
     8101 cccaaaaaac ccgagcgatg ccccaaaaat ctgcgtaatc tgtgtaaatc tgtggataaa
     8161 aacccgagca gtgccatccc gtgtgttcgt ggttaaatga tcgatccc
//
LOCUS       DAOSLE010000124         8195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8153_length_8195_cov_9.278624, whole genome shotgun sequence.
ACCESSION   DAOSLE010000124 DAOSLE010000000
VERSION     DAOSLE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8153_length_8195_cov_9.278624"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            396..1277
                     /locus_tag="PK576_08370"
     CDS             396..1277
                     /locus_tag="PK576_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE61645.1"
                     /translation="MRLFDAHTHLHDSRLASVFDAVIQKAEEAGITGLCSCGTAPVDW
                     ADVASLAERAGPFHIVPAFGVHPWFVAKLPDDWLEQLEPFLMRYANAPVGEIGLDGVR
                     REIPAARQEEVFVRQLDLAVRLQRPVVLHGARAWEAVLRTLKPYASRLPGILAHGFSG
                     SAEHLSRLLELGGHVSLGGTVCNPQAATVRTVAQCVPEDRLLLETDSPDLLPQTGLSA
                     GLGPDGKPLNQPSNLMRVCEAVAALRDLDPLHLAERTYANALAFFPQTAAGPCGRPLP
                     ARTSRVCVPTYTSALGV"
     gene            complement(1253..2062)
                     /locus_tag="PK576_08375"
     CDS             complement(1253..2062)
                     /locus_tag="PK576_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE61646.1"
                     /translation="MILHAESSGSGPDVVILHGLMGSCENWRSVRKILAGHYRVACLD
                     LPNHGRSPHVAQMEARAIGKMVLSTLDAFGMNRAVVVGHSLGGKIAMQMASEHADRLA
                     GVAVVDISPRAFQPMHLFILRACQQLDLVAAVRRVDLDHALAASVPHAETRAFLLKNV
                     VRDPKGRWAWRVPLQTLIDNYRVVSDAPPLVAPYNGPTLFVAGECSPFRIRDDESLIR
                     SWFPAVRWVTIPNAGHLVHADQPEAFTAALAGFLGTCYPSAELNQTPSAEV"
     gene            complement(2059..3216)
                     /locus_tag="PK576_08380"
     CDS             complement(2059..3216)
                     /locus_tag="PK576_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOE61647.1"
                     /translation="MAKRGFNIDPVTLRSMPFWCVGNPLGDPFGGPVLPKIDSLEVAE
                     LLAEAAGEGLIEATSFHDDDLVPWDPAKPEDDLDPKSETYAKLRAIKRVLDKAGLKTN
                     VATCGLHGDKLFRKGGLTNPDPKVRALAKKKVQRALRIGALFGAKHFTYWVARDGFEV
                     PVNVDWKNVYKWLADGLNGVYDYIRKMKFKNYIGATIEPKPNEPRGHMFLPTSGHAVG
                     FIYGHLKHPEFYGVNPELLQHESMTLLNAVHTVGYLTSMKKLFFLHFGSQIKGQFDND
                     FPPLIGPEGLKETVQMFWALRKIGWKGVVEYDCHMLRTDGDPDDPIGCRRAFIRDCTR
                     ALAMALLLADRLMDFDPAGMRDAEADLTATAMLCRLDPKSIRSWMTTVPGL"
     gene            complement(3300..4880)
                     /locus_tag="PK576_08385"
     CDS             complement(3300..4880)
                     /locus_tag="PK576_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE61648.1"
                     /translation="MLRYRAVLTRIAAVGLAGWVARAQSPFTLTLESTAVTPEHAEVR
                     VVLGVPEKHLIYAETFRVSAEPVRVPQAETKPDPIDPSRRVGVYAKTFESLWRVAPLR
                     DGTELTVAYQGCDDQVCFLPEEHVFRFDRQTGRFVATVANADAEAERTVDRFWTQGLR
                     MVTGGGYMGTGAFLAFLDQAETGTSGTAPAGNGWGGFLRDPVAFFHAHGLALTLVLVL
                     IGGLLLNLTPCVLPMIPINLAILGAGAGTRGRGFALGAAYGAGIVLVYGGLGWLILRT
                     GLFFGALQASPWFSLAVALIFTALTLALFDVFVIDFTRFFTPKNGGARQGALAAFSAG
                     ALSALLAGACVAPVVLAVLLLAGTLYAGGATGAQMLPFVLGLGMALPWPFAGAGLSVL
                     PAPGLWMVRVKQAFGVFLALLAAYYFHLAAVGFRSGGDAVAREGSIAAGDRAAWEAAV
                     ARARRAGKPIFVDFWATWCKNCTVMERTTFADERVRARLATYEVVKVQAEKPDREPAR
                     GMLEAFGVRGLPGFAVLK"
     gene            complement(4984..6591)
                     /locus_tag="PK576_08390"
     CDS             complement(4984..6591)
                     /locus_tag="PK576_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61649.1"
                     /translation="MLKTSKGMLVTLLAVGWLSRDTLAGEDVRVETNKLACMVRYEDF
                     GARGDGRTDDTAAIVRAHAYANRRGLPVQANASATYYIGGQDTPVIIQTDTDLGSARF
                     IIDDRKVENRHTHVFSVYSALPSTQPKGITSLKSGQRRINIELPKTCVVCVTDSNTKR
                     YIRRGLNQNAGSSQTDVFLVDKNGRVDERTPILWDFDQITHMVAYPVDEAPLRINGGR
                     FTTLANAAESEYIYYARGISIRRSNVVIDGLEHRITGEGDHGAPYAGFITIRDCANVT
                     VRNTTLNGHKTYQTIGSAGKPVFMGSYDISVTRALNVSFINCRQFDEINDHTRWGIMG
                     SNYSKNLLYDRCELSRFDAHQGVAGVTIRNSVLGHAGINAIGSGMLVVENTTVYGRSF
                     INLRSDYGSTWQGEFVIRNCTFVPAGGRPVSASLIGGSNDGQHDFGYACCMPSRIDIN
                     GLRIDDSRHPKGYQGPTVFADFNRSFKNAAYEEKFPYVKSREVILENITTVSGKPLHI
                     SSNPVMFKDVAVKHNGKANKPAGGDSQ"
     gene            complement(6676..7914)
                     /locus_tag="PK576_08395"
     CDS             complement(6676..7914)
                     /locus_tag="PK576_08395"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOE61650.1"
                     /translation="MKKRRTGCFWGIMCGWVGVVISAGAQEQTAMICGAPVRQARAEI
                     LWTRVICKQPGRYIGWPSVCVRKNGEVLAVFSGDRDEHVCPFGKVQMVRSTDQGATWT
                     TAQTICNTQLDDRDAGIVELPNGDLVVAWFTSVAYMGSIRDRSKLKPGSPQFYWWLHN
                     EKLPHAVKQEWLGYFTVRSSDGGKTWEAPVKTHGSANHGPIVLKDGRLLMVGRRWLGD
                     GTSAVWKEGKHELTVEESRDNGRSWQLIAMIEPTPPDEIGQFHEPHLVETDDGRLVAQ
                     FRFHAKPAPGAKHRDEAQCKLRQAESRDGGKTWTRMVPTEMQGYPPHLIKLRGGRLLT
                     VYGRRLGPGFGEYACLSDDHGRTWDVANEIKLAGHFNGDLGYPASVQLPDDTILTVYY
                     QAEKQGEKPCLMATRWRVRE"
BASE COUNT         1624 a         2688 c         2301 g         1582 t
ORIGIN      
        1 gtccctaccg gttggcggac gctttagtcc cgcatacgca ccggtctcca aaactcagtc
       61 gattgcgctt gcgatgaagt ggagcgacga agggctcgaa gggttttgtg acatgagcgc
      121 cccgcccatg aaacacgggc aagatgcctg tgccatgccg tccgcgctcc tgacgtcttg
      181 gcggttccaa aaatccgaaa cccgaagcca aaaaatttgc ccgtccttga tttcatgaag
      241 tttcatgtgt ttcatgattc ggaaccagta tcttccccca attgacagtt ttacgacctg
      301 acgtataatg aggcggcaac gatcacggac gcaaggtgga gtattgaact acagcggcac
      361 gatgattctg gctctataag gtaacccgtg gagtcatgag actgtttgat gcacacaccc
      421 atctgcacga ctcccggctg gcatcggtct tcgatgccgt gatccagaag gcggaggagg
      481 cgggcattac cggactctgc tcctgcggca ccgcgcctgt ggattgggca gatgtggcgt
      541 cgcttgcgga acgggcgggc ccgttccaca tcgtgcccgc tttcggagtg cacccctggt
      601 tcgtcgcaaa gctgccagat gactggcttg agcaattgga accctttttg atgcggtacg
      661 cgaacgcacc ggtcggcgag atcgggctgg acggggtgcg ccgcgaaatt ccggccgcgc
      721 ggcaagagga ggtgtttgtc cggcagcttg atctggcggt gcggctacag cggcctgtgg
      781 tgctgcacgg cgcacgcgcc tgggaagccg tgttgcggac cctcaagccc tacgcgtcgc
      841 ggctacccgg tatcctggcg cacgggttca gcggatccgc agagcatttg agccgtctgc
      901 tcgaactggg aggccatgtg tcgctcggcg ggacggtctg caatccccaa gccgctacag
      961 tccggaccgt ggcgcaatgc gttcccgaag atcggctgtt gctggagacc gactctccgg
     1021 atcttctgcc ccagacggga ctgtcggcgg ggctgggtcc agatggcaaa ccgctgaacc
     1081 aaccctccaa tctgatgcgg gtctgcgaag cggtggcagc cctccgcgat ctcgatcccc
     1141 tgcatctggc cgaacggaca tacgccaacg ctctggcgtt ttttccgcaa accgccgccg
     1201 gcccatgcgg ccggcccttg cctgcgcgga cgtctcgggt atgcgtccca acctatactt
     1261 cagcgctcgg cgtttgattc agctcagcag acggataaca agtaccgaga aacccggcca
     1321 atgccgcggt aaaggcttca ggttggtcgg catgcaccag atggccggcg ttcgggatgg
     1381 tgacccaccg gacagcggga aaccaactgc ggatcaggga ctcgtcgtca cggatgcgga
     1441 acggcgagca ctccccggcc acgaacaggg tcggcccgtt gtacggcgca accagcggcg
     1501 gcgcgtcgct caccacacgg tagttgtcga tgagcgtctg aagcggcacg cgccaggccc
     1561 agcgcccctt cggatcgcgc accacgtttt tgagcaggaa cgcgcgtgtc tcggcatgcg
     1621 gcacggatgc cgccagcgcg tggtccagat cgacacgccg cacggcggcg accagatcca
     1681 gttgctggca ggcccgcagg atgaaaaggt gcatcggctg aaacgcgcgc ggcgagatat
     1741 ccaccaccgc caccccggcc agacggtcgg catgttccga ggccatctgc atcgcgatct
     1801 tgccgcccag cgagtgcccg accaccacgg cacggttcat cccgaaggca tcaagcgtcg
     1861 acagcaccat cttgccgatc gcacgcgcct ccatctgcgc gacatggggc gaacgcccgt
     1921 gattcggcag atcaaggcag gccacacgat agtgcccggc cagtatcttg cgcacgctcc
     1981 gccaattttc gcacgagccc atcagcccgt gcagaatcac cacgtccggc cctgatccgc
     2041 tactctccgc atgcaggatc ataaaccggg gacggttgtc atccacgacc ggatggattt
     2101 cggatcaagg cggcacagca tggcggtagc cgtcagatcc gcctccgcgt cccgcatgcc
     2161 tgcgggatcg aaatccatca ggcggtcggc cagcagcagg gccatggcca gcgcccgcgt
     2221 gcaatcccgg atgaacgcgc gacggcaccc gatcggatca tccggatcgc catcggtccg
     2281 cagcatgtgg caatcgtatt ccaccacgcc tttccagcca atcttgcgga gcgcccagaa
     2341 catctgcacc gtttccttga gcccttcggg gccgatcagc ggcgggaagt cgttgtcgaa
     2401 ctgccctttg atctggctgc cgaaatgcag gaagaagagt ttcttcatgc tggtcaaata
     2461 accgaccgta tgcacagcat tcaggagcgt catgctctca tgctgcaaaa gctccggatt
     2521 cacgccataa aactccggat gcttcaagtg cccatagatg aagcccacgg catgacccga
     2581 tgtcggcagg aacatgtggc cgcgcggctc gttcggcttg ggctcgatcg tcgcgccgat
     2641 gtagttcttg aacttcatct tccggatgta gtcatagacg ccgttcaagc cgtctgccag
     2701 ccacttgtaa acattcttcc aatccacatt cacaggcacc tcgaagccgt cacgcgccac
     2761 ccagtaagtg aagtgcttgg ctccgaacaa cgcgccaatg cgcagcgcac gctgcacctt
     2821 ctttttagcc agtgcgcgga ccttcggatc agggttggtc aggccaccct tacgaaagag
     2881 tttgtcaccg tgcaggccgc aggtggcgac attggtcttg aggccggctt tgtccaacac
     2941 ccgtttgatc gcgcgcagtt tggcgtacgt ttcgctcttc ggatccagat cgtcttcggg
     3001 cttcgccgga tcccacggca cgaggtcgtc atcatgaaac gacgtcgctt cgatcaagcc
     3061 ctcgcccgcc gcttcggcga gcagttccgc cacctccagc gaatcgatct tcgggagcac
     3121 cggcccgccg aacggatcac ccagcggatt gccgacacac cagaacggca tggagcgaag
     3181 tgtaacgggg tcgatattga atcctctttt tgccattgcg cctctcctgt tttcctgtgt
     3241 gcccgtttgc acaggcgttt cccgcggccg catcagaccg ccttcccaac tctgagccct
     3301 tacttcaaca ccgcgaaacc gggcagtccg cgtacgccga acgcttccag catcccgcgc
     3361 gcgggctcgc ggtccggttt ttctgcctgc accttcacca cttcataggt tgcgagccgc
     3421 gcgcgcacac gctcatccgc gaacgtcgtc cgctccatca ccgtgcagtt cttacaccag
     3481 gtggcccaga aatcaacgaa aatcggcttg cccgcacggc gcgcccgggc aaccgcggct
     3541 tcccatgccg cgcggtctcc ggccgcgatc gacccctcgc gcgccaccgc atcccctccc
     3601 gaacggaacc ctacggcggc aaggtggaag tagtacgccg ccaacagggc cagaaacacg
     3661 ccaaaggcct gcttcacacg gaccatccag agaccgggcg cgggcagcac ggagagtccg
     3721 gcgcctgcga acggccacgg caaggccatg cccaacccca gcacgaaagg caacatctgc
     3781 gcacccgtgg caccgcccgc atagagcgtt ccggctaaca gcagcaccgc aagcacaacc
     3841 ggcgcgacgc aagccccggc cagcagggcc gaaagcgcgc ctgcggagaa ggccgctagc
     3901 gcaccctgac gcgcaccacc gtttttcggc gtgaagaacc gcgtgaaatc gatcacgaac
     3961 acatcgaaca gtgccagcgt cagcgcagtg aaaatcagcg ccaccgccag actgaaccac
     4021 ggcgaggcct gcaacgcgcc gaaaaacagt ccggtacgca ggatcagcca ccccagtccg
     4081 ccgtagacca gcacgatccc ggcaccatat gccgcaccca acgcgaaccc gcggccgcgt
     4141 gttcccgccc cggccccgag gatcgcgaga ttgatcggga tcatgggcag cacgcatggc
     4201 gtcagattga gcagcaaccc gccaatcagc accagcacga gggtcagcgc caatccatgc
     4261 gcgtggaaaa aggccaccgg gtcgcgcaga aaaccgcccc acccgtttcc agcgggagcc
     4321 gtcccgcttg ttccagtctc ggcctgatcc aaaaacgcca ggaacgcccc tgttcccatg
     4381 tatccgccgc ctgtcaccat ccgaagcccc tgcgtccaga aacgatcgac ggtccgctcc
     4441 gcttccgcat ccgcattggc caccgtcgcg acaaaccgtc ctgtctgccg gtcaaaacgg
     4501 aacacatgct cctcgggcag gaaacacacc tgatcgtcgc aaccctgata cgctaccgtc
     4561 agttccgtgc cgtcacgcaa cggcgcgacc cgccataggg actcgaaggt tttcgcatac
     4621 acgccgaccc gccgggacgg gtcgatcggg tccggcttgg tctccgcctg cggcacccgc
     4681 acgggttcgg cactgacacg gaacgtctcc gcgtaaatga ggtgtttttc cggaacgccc
     4741 aacaccacgc gcacttcggc gtgctccggc gtcacagccg tactctccag ggtcagggtg
     4801 aatggcgatt gcgcacgcgc cacccatccc gcgagcccta cggccgcgat ccgagtcaga
     4861 actgcgcgat aacggagcat acacacctct ccataaacgg tttccttcag tgtagcgact
     4921 tgccggtgcg ggttccacca aaaaacgcac ctgcggaacg gacgcccaac acgaagacgc
     4981 aagttattgg gagtcgccgc cagcgggctt gttggccttc ccgttgtgct ttacggcaac
     5041 atccttgaac atgactggat tgctgctgat gtggagcggc ttgccgctta ccgtggtgat
     5101 gttctcgagg atcacttcac gacttttgac gtaggggaat ttctcttcgt aagcggcgtt
     5161 tttgaagctc cgattgaagt ctgcgaaaac agttggcccc tgatagccct tcggatgccg
     5221 ggagtcatcg atacgcagcc cgttgatatc gattcgactt ggcatgcagc aggcgtatcc
     5281 aaagtcatgt tgcccgtcat ttgaaccgcc aatcaggctg gcgctgaccg gcctgccgcc
     5341 tgcgggaaca aatgtgcagt tccgaatgac aaactcacct tgccaagtac tgccgtagtc
     5401 agaacgcaaa tttatgaacg acctgccgta gacggtggtg ttctccacga cgagcatccc
     5461 gctgccgatg gcatttattc ccgcatgccc cagaaccgag ttgcgaatcg tcaccccggc
     5521 cacaccctga tgggcgtcga atcgcgacag ttcacatcga tcatacaaga gatttttgct
     5581 gtagttcgac cccatgatcc cccagcgggt gtggtcgttg atctcatcga actggcgaca
     5641 gtttataaag gacacgttca aggctcgagt aacggaaata tcgtaactgc ccatgaacac
     5701 cggcttgccc gcactgccga tcgtctggta ggtcttgtgt ccgttcagcg tggtgttgcg
     5761 caccgtcaca ttggcgcagt cgcggatggt gatgaatccg gcgtacggcg caccgtgatc
     5821 gccttcgccg gtgatgcggt gctcaagacc gtcgatcacc acattggagc gtcggattga
     5881 gattccccgt gcataataga tgtattccga ctctgcggca ttggcaaggg ttgtaaaacg
     5941 gccaccgttg atcctcagcg gggcctcatc gaccggatag gcgaccatgt gggtaatctg
     6001 gtcaaaatcc cataagatgg gggtacgctc atcaaccctg ccattcttat cgacgagaaa
     6061 cacatcggtt tgcgaacttc cggcattctg attcagtccc ctccggatgt agcgcttggt
     6121 gttggaatcc gtgacgcaca ccacacaggt cttgggcagc tcaatgttta ttctgcgctg
     6181 gcccgacttc aaagaagtga tccccttggg ttgcgtggac ggcaacgctg agtaaacgga
     6241 aaacacgtgc gtgtgacggt tctccacttt gcgatcatca atgatgaatc gggcgctccc
     6301 caagtcggtg tccgtctgga tgatcaccgg ggtgtcttgg cccccgatgt agtaggtcgc
     6361 gctggcattc gcctgtaccg gcaacccccg ccggttggca tacgcatgcg ctcggacaat
     6421 cgccgcggtg tcatccgtcc tgccgtcccc ccgtgccccg aaatcctcgt atcgcaccat
     6481 gcacgccaac ttgttcgttt ccaccctcac gtcctctccc gcgagggtat cccgactaag
     6541 ccatccgacg gctagtagcg taaccagcat gcctttgctt gttttcaaca tatgaaaaac
     6601 aacttcccct acccttccgc tgctaaccgc gacatgattc cggcggaaaa ccgattcgtt
     6661 gtcgttccgt tcacgtcact ccctgacacg ccaccgcgtc gccatcaggc aaggcttctc
     6721 cccctgcttc tctgcctgat agtagaccgt aaggatcgtg tcatccggaa gctgaaccga
     6781 agcgggataa ccgagatcgc cgttgaagtg gccagccaac ttgatttcgt tggcaacatc
     6841 ccacgtccgc ccgtgatcgt cactgaggca cgcatactca ccgaagccgg ggccgagcct
     6901 gcggccgtag accgtcagca gacggccgcc acgcaacttg atcaggtgcg gcggataccc
     6961 ctgcatctcg gtgggcacca tgcgcgtcca cgttttgcct ccgtcgcggc tctcggcctg
     7021 ccgcagcttg cattgtgcct cgtcgcggtg cttcgcgccc ggcgcgggtt tggcgtggaa
     7081 ccggaactgc gccaccaaac ggccgtcatc ggtctccacc agatgcggct cgtgaaactg
     7141 gccgatctca tcaggcggtg tgggttcgat catcgcgatg agttgccaag agcgaccgtt
     7201 gtcgcgtgac tcttcgacgg tcagttcatg cttaccttcc ttccacacgg cagaggtacc
     7261 gtcgcctagc cagcgccgcc ccaccatcaa cagccgtcca tctttcaaca cgatcggacc
     7321 gtgattggcg gaaccatggg ttttcaccgg tgcctcccac gtcttgccgc cgtcactgga
     7381 gcgcaccgtg aaataaccga gccactcctg cttgaccgcg tgcggcagct tctcattgtg
     7441 aagccaccag tagaactgcg gcgagccggg cttgagcttg ctccggtcac gaatacttcc
     7501 catgtaggcc accgaggtga accacgccac gaccaaatcg ccgttgggca gttcaacaat
     7561 acctgcgtcg cgatcatcaa gctgcgtatt acagatggtt tgcgccgtgg tccaggtcgc
     7621 gccctgatct gtgctgcgca ccatctggac cttgccgaag ggacaaacgt gctcatcgcg
     7681 gtcgcctgaa aaaaccgcca gcacctcacc gtttttgcgc acgcataccg acggccagcc
     7741 gatgtaccgg cccggctgtt tgcagatcac ccgggtccag aggatctcgg cccgcgcctg
     7801 ccgcaccggc gcgccgcaga tcatggccgt ctgctcctgc gccccggccg atatcaccac
     7861 accgacccat ccgcacatta tcccccaaaa acaacccgtt cttctttttt tcattgtcga
     7921 gccccttcgt tatcccgcgt agtttagtcg gcacctctgt cgtagtcaag cgccccacag
     7981 cttccggcgc atccgttgtg ttgaccgtgg ccagacttta gcccaaaagg aaccgttgaa
     8041 cgggtaaaat tctagcaaag ccccgtaaac attgggtttt gacggattga gatgcgggcg
     8101 gagcccgcaa tctcaaaggc tgtgagactg tgaggctgtg aggctgtgag gtggtggtat
     8161 tccgtggtta aatgatcgat cccgatcccg atagc
//
LOCUS       DAOSLE010000125         8188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8160_length_8188_cov_7.185909, whole genome shotgun sequence.
ACCESSION   DAOSLE010000125 DAOSLE010000000
VERSION     DAOSLE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8160_length_8188_cov_7.185909"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..1794)
                     /locus_tag="PK576_08400"
     CDS             complement(25..1794)
                     /locus_tag="PK576_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOE61651.1"
                     /translation="MMKTMLALGWGFGVVILACAGERVALWPEGKIPDFQPRQIAATT
                     QEVKEPGFKPAERVMPYLDWYEAPAQKNGACMLLISGGGYQSCCDGVWIDRVAKKLID
                     LGFVCVSLTYRTPRPQGLPIYQSGWQDGQRAVRLVRNEAQKRGFDPEKIGAFGFSAGS
                     HLTVLLATSALTPAYGPVDDLDRLPCHINWAVPIYTAYALTDGLTGPNTRDGDAIDVK
                     LTDVFKFDAKTCSMALFHGGMDAYSPNGSTQIYRQLRRMKIPAEIHLFADRPHGFMGN
                     PDKGEQGTAYDNWLDRIAEYLRQMNFDGRLGEEVDLMTRYPNDDARDGYRKEPVWPEG
                     RMPDVQTNQGLPYLEWHMPKERKTKAIQIIYSGGGYGGNSPDGFEVAPTRRFLNEKGM
                     TVVTMKYRTPRPLGGLAKHTTAWQDLQRAVRVVRSQAAALGLDPDRIGIMGSSAGGHL
                     TLMGATSSKRRSYWPIDDLDKIPCNVQWAVAIYPAYALTDGSNAPNAKGGNEDDSRPV
                     PEFSFDLATCPILFIHGDADSWAPMNSVKCWEQLRRMGIQSDLHTLATRNHCFQRKAS
                     PGTGSYTWMGRIWEFMNHKGFNK"
     gene            complement(1809..2855)
                     /locus_tag="PK576_08405"
     CDS             complement(1809..2855)
                     /locus_tag="PK576_08405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE61652.1"
                     /translation="MRPLIRLNVSLLLVLVCCWSGCGKKSEPAAEVESAANSAPTAGV
                     TSPDYVVAKVNGTPLTWEEMDRRAMGYLKDDVEVNHLVIPSNRLEEAREHFRRRAINA
                     FVFKTVMLDEALKRKIAVTEADRQAGLRALQNTLKSRNWTTNDFFERGPQPPRMMRRE
                     FEDGLIIDKLLKREVRDKIRVEEAELTEAIGVLHETNQLRRARLETIRKQLLDGADFA
                     DMARNVSECPSAKDGGDLGEFARGKKVKPFEDAAFSQKIGEIGPVIETRFGYHIIKTT
                     SRMPAKVATSTTPAVPETVRASHILIKSVPVNRRRLSDTILRRKYNTGVETFFAELKK
                     KAEITCYLYQDMTF"
     gene            complement(2880..5093)
                     /gene="pflB"
                     /locus_tag="PK576_08410"
     CDS             complement(2880..5093)
                     /gene="pflB"
                     /locus_tag="PK576_08410"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOE61653.1"
                     /translation="MSLSSDIWRININVRDHIISHYTPYDGDDAFLAPVTGRTRKLWD
                     QVRGLMEEERARNGIYDIDNSRISTITSHAPGYIDRELEQIVGIQTDTPLQRAIMPFG
                     GIRMVESSLKAYGRELPQAIQDVFRFRKTHNDGVFDAYSDEMKLARHNGIITGLPDAY
                     GRGRIIGDYRRVPLYGLDFLIAHKEAAKARHVFDAMDMEAIQLREEISEQIRALQELK
                     AMAASYGYDIAQPARDTREAIQWLYFAYLAAVKEQNGAAMSIGRISTFLDIYAERDLA
                     AGRYTEAEIQEFVDHFVMKLRMVRFLRTPNYDALFSGDPTWITESLAGIGVDGRHMVT
                     KMTYRFMHTLVNLGPAPEPNMTVLWSPRLPEPFKRFCARLSIATSSIQYENDELMREK
                     FGDDYGIACCVSAMRIGKQMQFFGARANLAKALLYCINGGKDEITGQQVGPEMAPVTS
                     DVLDYEETVRKFDQICRWLSRLYINTLNVIHSMHDKYCYERLQMALHDEDVFRTAACG
                     IAGLSVVADSFSAIKHAKVRPIRDARGLAVDFEIEGEYPTFGNNDPRVDEIAVEVIKT
                     FMGRLSKHRTYRHAKPTLSILTITSNVVYGKTTGSTPDGRKKGEPFAPGANPMHGRDS
                     RGCIASMKSVAKLPYDHSEDGISFTFSIVPNALGRTPGERVENLVGLLDGYFSEKGHH
                     INVNVLEREMLLDAMEHPENYPQLTIRVSGYAVNFIKLTREQQLDVIHRTFHKTF"
     gene            complement(5144..5220)
                     /locus_tag="PK576_08415"
     tRNA            complement(5144..5220)
                     /locus_tag="PK576_08415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5184..5186),aa:Arg,seq:ccg)
     gene            complement(5322..6983)
                     /locus_tag="PK576_08420"
     CDS             complement(5322..6983)
                     /locus_tag="PK576_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOE61654.1"
                     /translation="MFIYHFNRLIRSRILWGFFVIVIAVAFVAVDSCFQRPQDRMAAG
                     KLNGKTVTSEEFDRVVQTIRGFGQNRDNTTPASVVDRRAWEQLAARQVARKNGLAASR
                     DEIRAVLREAPGFQGPNGFDLNRYRAVLAEQGLTPTLYENMVSHQLAIMKNAALVETA
                     AWVAPMELEDELAAMTDIFTVQVAAVSNRFAEAAMPLTDADYRKYYEENKDSFALPDR
                     MSVRYVMIPVSNYLAAVSVPEDDLLEYYDSHANTYTRTTTNNTTEPIPFAEVRTNILA
                     ELMLEEARYCAETSVTFTVYGKLTAAGSNALDQISMQQRLPVKRSPLFGAEEALFWVE
                     DSKAFADAAFELDPERADSRFGIVKGQQFIYVIEPLERSEAHTPTYEAVLEELKPRAQ
                     AKARTEKFDNVAKTLREDLLKRLGEGKSFAEAAREKALNVTTALTYTVSDIQNQKFEN
                     SFSIAYGAMTLRKGELSEIIPVSAARALLVYVQDRQPGDALAAEMMRAQIRNAIARRR
                     NANLFDAWLSWNLSCQDFQPTRPLDDEEDGETEKITAPAEDDDAG"
     gene            complement(7084..8082)
                     /locus_tag="PK576_08425"
     CDS             complement(7084..8082)
                     /locus_tag="PK576_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61655.1"
                     /translation="MMDQTGLLRVGCCVMGLALLTALAQENAAPVAGEAVPAAAQESD
                     AAAVKVFDPLVRLMNVLGVCEVNIPGTEGFAPATNNKYYPLGTVFRIGMGGSALLVFS
                     AQESVKLTANTEVVVTAPEKNPEARVLRFSGGAMKTTLRDNLQEGLFAIETPSTTCRN
                     VAGRGEYTLVCDGTLETFQAATITGSALVEGAQYHIPALRAANTVNIQNAPDRTLTRL
                     TSVSGDFPIVLENGTETPVTFGMTPKAVVKIWRENAPVGGRPIISTLVVSPTGIARHR
                     FVYAEGRPDLTTGELIVPAEPEPKEEALPVLLDGVAQDSAAPAVPATPAADGQKEP"
BASE COUNT         1545 a         2555 c         2406 g         1682 t
ORIGIN      
        1 gaattgcggc ggttcagcgc cgtgtcactt gttgaacccc ttgtggttca tgaattccca
       61 gatacggccc atccaggtgt aactaccggt acccggggag gctttccgct ggaagcagtg
      121 gttgcgtgtg gccagcgtgt ggagatcgga ctggatcccc atccggcgaa gctgctccca
      181 gcatttcacc gaattcatcg gcgcccagct gtccgcgtcg ccgtggatga acagaatcgg
      241 acacgtcgcg aggtcgaacg agaattcggg aacgggccgc gaatcgtcct cgttgccgcc
      301 cttcgcgttc ggagcgttcg aaccgtccgt aagcgcgtac gccggataga tcgcgaccgc
      361 ccactgcacg ttacagggaa tcttatcgag gtcgtcgatc ggccaatacg aacgtcgttt
      421 cgaactggtc gcgcccatga gcgtcaagtg tccgccggcc gaggagccca tgataccgat
      481 gcggtcggga tcaagcccca acgccgcggc ctgactgcgg acgacccgca cggcgcgttg
      541 caagtcctgc catgcggtcg tgtgtttggc caatccgccc agcggacgcg gcgtgcgata
      601 tttcatcgtc acgaccgtca tacctttttc attcaggaag cgccgcgtcg gcgcgacctc
      661 gaaaccgtca ggactgttgc caccgtagcc gccgcccgaa tagatgattt ggatcgcctt
      721 tgtcttacgc tccttcggca tgtgccattc gagataaggc aaaccctgat tcgtctgcac
      781 atccggcatc cggccttccg gccagaccgg ttctttgcga tacccgtcgc gggcatcgtc
      841 attcggataa cgggtcatca ggtcgacttc ctcgccgagg cgtccgtcga aattcatctg
      901 gcgaaggtac tcggcgatcc ggtcgagcca attgtcgtag gcggtgcctt gttcaccctt
      961 gtctgggtta cccatgaagc cgtgcggccg atccgcgaaa aggtgaatct cggcggggat
     1021 tttcatcctg cggagttggc ggtaaatctg cgtggagccg ttcggcgaat aggcgtccat
     1081 cccgccgtgg aagagcgcca tcgagcaggt cttcgcatcg aacttaaaca cgtccgtgag
     1141 tttgacatcg atggcatcgc catcgcgcgt gttgggaccg gtcagcccgt ccgtgagcgc
     1201 gtaggcggtg tagatcggca cggcccagtt gatgtggcac ggaagccggt cgaggtcgtc
     1261 aaccggcccg tacgccggcg tgagtgcgct tgtggcgagc aagaccgtca ggtgcgagcc
     1321 tgcggaaaag ccgaacgcgc cgatcttctc cggatcgaat ccgcgcttct gcgcctcgtt
     1381 acgcacaagg cggacagcgc gctgtccgtc ctgccacccg ctttgataga tcggcagacc
     1441 ttgcggacgc ggtgtccgat aggtgaggct gacacagaca aacccgagat cgatcagctt
     1501 cttggcgacc cggtcaatcc agaccccgtc gcagcagctc tgataaccgc ctccggaaat
     1561 caacagcatg cacgcgccgt ttttttgtgc cggcgcctca taccagtcga gatacggcat
     1621 cacgcgctcc gccggtttga atcccggctc tttgacctcc tgcgtggtcg ccgcgatctg
     1681 ccgaggctgg aaatccggaa tcttcccttc cggccacagc gcgactcgct ctccggcaca
     1741 cgccagaatg acgaccccga acccccaccc caacgccagc attgttttca tcatttcgtt
     1801 tccttttttc agaaggtcat gtcctggtac aggtaacagg tgatctccgc cttctttttg
     1861 agctcggcaa agaaggtttc cacgcctgtg ttgtatttgc ggcgcagaat ggtgtcactc
     1921 aggcgcctcc ggttgacagg caccgatttg atcaaaatat gggacgcgcg gacggtttcg
     1981 ggcaccgcgg gcgtagtgga ggtcgcgact ttagccggca tacgggaggt ggtcttgatg
     2041 atgtgataac cgaagcgcgt ctcgatcacc ggcccgatct cgccgatctt ttgcgaaaag
     2101 gcggcgtcct caaacggctt gaccttcttg ccgcgtgcga attcaccgag atccccgccg
     2161 tccttggcac tcgggcactc ggaaacgtta cgcgccatat ccgcgaaatc cgccccgtcc
     2221 aacaactgtt tgcggatcgt ctccaatcgc gcgcgccgga gctggttggt ttcgtggagc
     2281 acccctatcg cttccgtcaa ttccgcttct tcgacgcgaa tcttgtcccg cacctcacgt
     2341 ttgagcagct tgtcgatgat cagaccgtcc tcgaactcgc gccgcatcat ccgggggggt
     2401 tgcggtcccc tctcgaaaaa atcgttggtg gtccagttgc gggacttgag cgtgttctgc
     2461 aacgcacgca gaccggcctg ccgatccgcc tcggtcaccg cgatcttccg cttcaaggcc
     2521 tcatccagca tgacggtttt gaacacgaag gcgttgatcg cgcgacggcg gaagtgttcg
     2581 cgggcctctt ccagccggtt ggacgggatg accagatggt tgacctccac gtcatccttg
     2641 agatagccca tggcgcggcg gtccatctcc tcccacgtca gcggcgtccc gttgactttc
     2701 gcgaccacat agtccggcga cgtgacaccg gcggtcggcg cggaattcgc ggcggactcc
     2761 acttcagcgg cgggttcgga tttcttgccg catccgctcc agcaacacac cagcaccagc
     2821 aacaggctga cattcagacg gatcaaaggt cgcatacggt ttctcctcgc tcggggggtt
     2881 cagaaggtct tatggaaggt acggtgaatc acatccaact gctgttcgcg cgtgagttta
     2941 atgaagttca ccgcatatcc ggagacgcgg atcgtgagtt gcggatagtt ctccggatgc
     3001 tccatggcat ccagcagcat ctcgcgctcc agcacgttca cattgatgtg gtggcccttc
     3061 tcgctgaagt aaccgtccag caggccgacc aggttttcga cccgctcgcc gggtgtgcgg
     3121 cccagcgcgt tcggcacgat cgagaaggtg aaggaaatcc cgtcctcgct gtggtcgtag
     3181 ggcagtttcg ccaccgattt catggaagcg atgcacccgc gtgaatcgcg gccgtgcatg
     3241 ggattcgcgc cgggcgcgaa cggttcgccc tttttgcgcc catccggcgt cgaaccggtt
     3301 gttttgccgt acactacgtt ggaggtgatg gtgaggatcg aaagcgtggg cttggcgtgg
     3361 cggtaggtgc gatgtttcga aagccgcccc atgaaggtct tgatcacttc gacggcgatc
     3421 tcgtccaccc ggggatcgtt gtttccgaag gtgggatatt ccccctcgat ctcgaaatcg
     3481 acggccaaac cgcgcgcgtc ccggatcgga cggaccttgg cgtgcttgat cgccgaaaaa
     3541 ctgtccgcca ccaccgacaa cccggcaatg ccgcaggctg ccgtacggaa gacatcctca
     3601 tcgtgcaacg ccatctgcaa ccgctcataa cagtacttgt catgcatact gtggatgaca
     3661 ttgagggtat tgatgtagag ccgggagagc caacggcaga tctggtcgaa tttccgcacg
     3721 gtttcctcat aatcgagcac gtcggaggtg accggggcca tctcggggcc gacctgctgc
     3781 cccgttatct catccttgcc gccgttgatg cagtacagca gggctttcgc cagattggcc
     3841 cgcgcaccga aaaactgcat ctgcttgccg atccgcattg cggaaacgca acaggcgatc
     3901 ccgtaatcat ccccgaactt ctcccgcatc agttcgtcat tctcgtactg gatggacgag
     3961 gtcgcaatgg agagccgggc gcagaaccgc ttgaagggct cgggcaggcg cgggctccac
     4021 agcaccgtca tgttcggttc gggggccggc ccgagattga ccagcgtgtg catgaaccgg
     4081 taggtcatct tggtgaccat gtgccggccg tccacgccga tcccggcgag cgattccgtg
     4141 atccaggtgg ggtcacccga gaaaagcgcg tcataattgg gcgtccgcag aaaccgcacc
     4201 atccggagtt tcatcacaaa gtggtcgaca aactcctgga tttcggcctc ggtgtaccgc
     4261 ccggcagcca gatcgcgttc cgcatagatg tccagaaagg tcgagatgcg gccgatcgac
     4321 atggccgcgc cgttctgctc tttgactgcg gcgagatagg cgaaatagag ccattggatc
     4381 gcctcgcggg tatcccgcgc gggctgcgcg atgtcatagc cgtaggaggc ggccattgcc
     4441 ttgagttcct gcaacgcccg gatctgttcc gagatttcct cccgcaactg gatcgcctcc
     4501 atgtccatgg cgtcaaagac atggcgcgcc ttggccgcct ctttatgcgc gatcaggaaa
     4561 tccagtccgt acagcggcac gcgccggtaa tcgccgatga tgcgcccccg gccgtaggcg
     4621 tcgggcagac cggtgatgat gccgttgtga cgcgcgagct tcatctcatc cgagtacgcg
     4681 tcaaacaccc cgtcattatg ggtcttgcgg aacctgaaga catcttggat cgcctgcggc
     4741 agctcccggc cgtacgcctt gagggaggac tcgaccatac ggatgccgcc aaagggcata
     4801 atggcccgct gaagcggcgt gtcggtctgg ataccgacga tctgttcgag ttcgcggtcg
     4861 atgtatccgg gcgcgtgcga ggtgatggtc gagatgcggc tgttgtcaat gtcgtaaatg
     4921 ccgtttcggg cgcgttcctc ctccatcagg cctcgcacct gatcccaaag tttccgggtc
     4981 cgcccggtca ccggggcgag aaacgcgtca tccccgtcgt acggggtata atgtgaaatg
     5041 atgtgatccc ggacattgat gttaatccgc cagatatctg aagagagcga catgcaaccc
     5101 cccctgtccg atcccgcgcc accgcgacgc ttcatgttga aactggtgcg cctggcgggg
     5161 gtcgaaccca caaccttcgg cttcggaggc cgacactcta tccggttgag ctacaggcgc
     5221 aaaaagtggg ggttatgata atgactgtca ggtcggactt caagcccgtt gttcatcccg
     5281 gaaccgaaaa tccgaggcac acggcacagt aagccccctg ttcagcccgc gtcgtcgtct
     5341 tccgcaggag cggtgatctt ctccgtttcc ccatcctcct catcatcaag aggacgggtc
     5401 ggttggaaat cctgacagga gagattccag gagagccatg cgtcgaacag gttggcgttg
     5461 cggcggcgcg cgatggcgtt ccggatttgc gcgcgcatca tctcggccgc cagtgcgtca
     5521 cccggctggc ggtcctgcac ataaaccaga agcgcgcggg ccgcggagac cggaatgatc
     5581 tcggaaagct cgcccttgcg cagcgtcatg gctccgtagg cgatcgagaa actgttctca
     5641 aacttctggt tttggatgtc actgaccgta tacgtgaggg ccgtggtgac gttgagcgct
     5701 ttttcccggg cagcttcggc gaacgatttg ccttcgccga ggcgcttcaa cagatcctcc
     5761 cgcaacgtct tggcaacatt gtcgaacttc tcggtacgcg ccttggcttg ggctctcggc
     5821 ttcaactctt ccaagaccgc ctcatacgtc ggcgtatgcg cttccgaacg ttccagcggc
     5881 tcgatcacgt aaatgaactg ctgccccttg acgataccga agcgtgaatc cgcgcgctcg
     5941 ggatccaatt cgaaggcggc atccgcaaat gccttgctgt cctcgaccca gaacaacgcc
     6001 tcctcagcgc cgaagagcgg agaacgtttg acgggcaggc gttgctgcat tgaaatctga
     6061 tcgagcgcat tggatcccgc cgcggtcagc ttgccgtaca cggtgaaggt gacactcgtc
     6121 tccgcgcagt accgggcctc ctccagcatc agttcggcga gaatgttcgt gcgcacctcg
     6181 gcaaagggga tcggttccgt cgtgttgttg gtggtggtcc gggtataggt gttcgcgtgg
     6241 ctgtcgtaat attccagcag gtcatcctcg ggcacggaaa cggccgcgag ataattggag
     6301 accggaatca tgacataccg cacggacatc cggtccggca gggcgaacga gtccttgttc
     6361 tcctcataat actttcggta atcggcatcg gtcagcggca ttgcggcttc cgcgaagcgg
     6421 ttgctgactg cggcgacctg caccgtaaag atgtcggtca tggcggcgag ctcatcctcc
     6481 agctccatgg gcgcaaccca ggcggcggtt tcgacgagcg cggcgttttt catgattgcc
     6541 aactggtggc tcaccatgtt ctcatacagg gtgggcgtca agccctgctc ggcgagcacc
     6601 gcgcggtagc ggttgagatc aaacccgttg gggccctgaa aacccggcgc ctcacgcaag
     6661 accgcgcgga tctcgtcccg gctggccgcc aaaccatttt tccgcgccac ctgccgcgcc
     6721 gccaactgtt cccatgcacg gcggtccacc acgctggcgg gggtcgtgtt gtcacggttc
     6781 tggccgaatc cgcggatcgt ctgcacaacc cgatcgaact cctcagaggt gaccgttttc
     6841 ccgttcagtt tcccggcggc catccgatcc tgaggccgct ggaaacagga atccacggcc
     6901 acaaacgcaa ccgcaatgac aataacgaag aagccccaga gaatccggct acggatgagg
     6961 cggttgaaat gataaatgaa cacgattttt ttccaaacgg ggttgagtac gcgcaacgtt
     7021 tatcacaaga aaaaccggtg gctgcgcagg ccgccggtca tcccccacaa cacgcccctt
     7081 cgatcagggc tccttctgcc cgtccgccgc aggcgtggcg ggcacggccg gggctgcgga
     7141 gtcctgcgcc acaccgtcca aaagaactgg caacgcctct tctttcggtt ccggttccgc
     7201 ggggacaatc aattcgccgg tggtcaggtc cggacgcccc tccgcgtaaa cgaaacggtg
     7261 ccgggcgatt ccggtggggc tcacaaccaa ggtcgagatg atcgggcggc caccgaccgg
     7321 cgcgttctca cgccagatct tcaccacggc tttgggagtc atgccgaagg tgaccggtgt
     7381 ctcggtgccg ttttccagca cgatcgggaa atcgcccgac acgctggtga gccgggtgag
     7441 cgtgcggtca ggcgcgttct gaatgttcac cgtattggcg gcgcgcagtg ccggaatgtg
     7501 gtattgcgcg ccctccacaa gtgccgaacc ggtgatcgtg gcggcctgaa aggtttccag
     7561 cgtaccatca caaaccagcg tgtactctcc ccgacccgcc acattgcggc aggtggtgct
     7621 gggggtctcg atcgcgaaca gcccctcctg aagattatcc cggagcgtcg tcttcatggc
     7681 gccgccgctg aaacgcagca cacgggcctc gggattcttt tccggcgctg tcaccacaac
     7741 ctccgtgttc gcggtgagct tgacgctctc ctgagcggag aacaccagca gggccgaccc
     7801 gcccatgcca atgcggaaca cggttcccaa cggatagtat ttgttgttcg tcgccggcgc
     7861 gaacccctcc gtccccggta tgttcacctc gcacacgccc aacacattca tcaggcgcac
     7921 gagcggatca aacaccttga cggcggccgc gtcactttcc tgtgccgccg caggcaccgc
     7981 ctcacccgca acaggtgccg cattttcctg agccagggcc gtcagcagag ctaaccccat
     8041 cacgcaacat cccacccgca acaatcctgt ctgatccatc atggggacga ctccctcgtt
     8101 cgcatgccgt ttcaacaaac cttctacctc aaataaaaaa gatgaggaat tttagatcaa
     8161 acccgtcaga tagtcaatcc gtgcttca
//
LOCUS       DAOSLE010000126         7865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8548_length_7865_cov_7.292830, whole genome shotgun sequence.
ACCESSION   DAOSLE010000126 DAOSLE010000000
VERSION     DAOSLE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8548_length_7865_cov_7.292830"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..3727
                     /locus_tag="PK576_08430"
     CDS             326..3727
                     /locus_tag="PK576_08430"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOE61656.1"
                     /translation="MKNSVFLWVAVTAMVIGYALAVRGAAVSVKIAEAAVVWNPETGF
                     ARYGKGRCQMALAGFTNMIDKVTGRVPAVYVEGQEPADLVSVIYFGNTKAARAAGCDA
                     QELRRGDWRIKSVPGKVFIFANSGMGLAYAAADFAERHCGYYYLTPEGDDPYDFNPAL
                     TIPVGDITVKPAIYNAKVYTAVGGTWNRWAINRRLSSTDEMEGQHRDSYEVRDFTGRT
                     RTCHTSFCYLPPEKHFKDHPEYYSLIKGKRQYTPVGQLCMSNEEVKRLCLEQLLTFIA
                     EDRRKYPDNYPLVYDFTQQDNMSFMCECPECKKIIDKYKRTSGDKNRTDNYWGGDAGL
                     QLEFINWIADRVAEKYPDVLIRTFAYVSTETPPKPGTIKPSPNVCIWWCDLYGESEHL
                     RPITAKPFNYKRGEQIREWLQLAKSVQVWDYMLYGGSHSGDFPEFAPDAIKADAKFFA
                     ENGLDDLFMESERSGIMPQPFYGLNYYLMSKLYTDPDEDVDALIKTYCRVYGKGAAKM
                     YEALQFLRRIEIENPAQTPGEWHQRVLPWRTRANGEAFRALVKSAYDQEGPGAARARM
                     ARVLSSVSRDLVRTLGQIPSAAAELKTAKKDFADYSFEYAKTASMTDQERKKMLADIQ
                     TEVELFDLKFKNIPKEVEGVPEDEIKCADYRRFSGSPNGKRMDDPDSETGKAFVWKYG
                     KPDAEHMGFPVSCGVYDGARKKNCAFKITAEMAGTDEQYHWIKLGKGWVGPQGMFWIP
                     HDWHMSFRFADWHILCDGLPSDPNWYDVYLSAKFTGPAYVPGSTRPNGIWIDRLLLRR
                     TQPPKENIVSPAKPGRADWMRGKYGLMVHWLFPSNRDIDRWTDAFNVDAFLKDFDKTG
                     AEWLIFTAGQCRGAYCSPSETFTKFCGPGHVPKRDLLAEIACGVKARGKRFIVYAACD
                     FVKDGCDDHSVQKGLCWDNSTEDRTEFERRWPLVLKEWAIRLEKNCDGWWLDGLGVQY
                     PNGVNWKLWCEACRAGNPAAAIAFNSGANNFSAYGPCDYTAGEASALKFALPDLDTAP
                     PAAAVRHHLFPIDGYWGGFWTWPTASWAGVQHLMPTRPELFDKAAMDARLEKRDFPDP
                     IYDVATLGAYLRKARETGSAATINIGISPEGRLNPKSIELIRAAR"
     gene            complement(3817..5220)
                     /locus_tag="PK576_08435"
     CDS             complement(3817..5220)
                     /locus_tag="PK576_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61657.1"
                     /translation="MSDGNRRDFLKGTAWMLSAAAAGCVNNRPFGEYPSVANFTVPPI
                     TKVRIGFVGVGERGTWAVKRISNIPGTETAALCDLRPQQIPPLIEYLNKKKSPGTLHT
                     YAGKDDSWKGLCDDAAVDIVYLCTPADLHARMAVYAMNAGKHVMVEVPAAKTLDDCWA
                     IVEASERNRVHCMMLENCVYGENELLAWNLCHQGLLGTLTHAEGGYIHLLTWRHLENH
                     FRNRHHQKPGTVVHTYGNSYPTHALGPICTYMDMNRGDRMEKVVSMSSLSAGHREFAR
                     HFYKPDDWQNNIEWRTGDMNTSIIRTALGRTIMMQIDMSTPRPYSRINLIQGTKGCFY
                     DYPLRITYSDEPGGQKGHEWFDKKKLENVRKLHMHPLYKDAGEIAKKIGGHGGMDFMM
                     DLRWVFCLQNGLPLDMDVYDLASWSSIVPCSADSDRRGGEVVELPDFTRGAWKTAKPI
                     SIGSIDITKMGLENAHR"
     gene            complement(5395..7761)
                     /locus_tag="PK576_08440"
     CDS             complement(5395..7761)
                     /locus_tag="PK576_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOE61658.1"
                     /translation="MMNITRRAFLGSAAMFTAAAGLRGENVPPVTPFDSAAYTALRKQ
                     LRAFYPDECRGVAKDPRQPESEKKIAAEVRAWANANPGFDALDIRRESYRAMTRHFVP
                     FLFTESPFYFEAGVNGGWSGARPARVVNAICGRFYKEKNLIPDEAFRLLRKRNGERMA
                     LCCGPFVDDMHHVPPIRVVLAKGFGGIRADVAAALETCPKNDPYGRKLLETALVGFDT
                     LRDLQLAFAREAQKRLEDPALDATGRRHFTRIAESAQRCPWEPPQTFFEGLNTLWFIR
                     EILGYTDGLMIYALGRPDAWLYDLYRADLAAGRLTEAEAADLVARFLITADCHHDGMI
                     PVDSYSDHEMEIPMSLGGCDKHGKPVYNELTRLFLDAHIGTDCVFPKLHCRIASDSPQ
                     EYLAKIGGMLTKGHAVFALLNDDRYIPQYIRQGFPVEDAREYTGAGCWNGYIDSVMDV
                     DGANYVSVMRTFELAIHRDPKVEREARIRIDPLDGVTSFEEARNIAYRNFIRFFRDVM
                     SEYTRYGAQNAKVFPHPVYSMCLRGGIQSRRDTTEGGIPARPRVITLGFIGNVVDSLL
                     AIKHLCFDTKTCTLPELLAAVRSNWSGPRGAELRKAAFAGPYWGDGTEQTCGEMAWWI
                     RRIHDDIEGFANDQGDAYRLAIYTYREFMYWGNATKATPDGRRDGDRLAQGFSPSEYR
                     CKEGVTTVLNAIGTLPHECLFASNANLTFDKTAMSPETFAAVFRVYAKKCGHLLQPNC
                     NSVEQLLEAQKHPERYQYLIVKVCGFSARFVALSKRWQDEVIQRHRLR"
BASE COUNT         1693 a         2326 c         2246 g         1600 t
ORIGIN      
        1 atctgcgtaa tctgtgtaaa tctgtggata aaaacccgag cagtgccatc ccgtgagttc
       61 gtggttaaat aatcgattcc gatagcgata gcgacaccga tcccgatttg gatgaaaccc
      121 tgaaaccttg aaaccatgaa accatgaaac cagaaaccct gaaaccctga aaccatgaaa
      181 ccatgaaacc atgaaaccaa agaaagttga aggttaaact gctcttttta ggattgactc
      241 ttttcacccc cctccgcata gtaagcggcg atcatggcaa ccggtgtgtt tccggtctac
      301 cctgacatcc attacgaagt tttttatgaa aaattcagtt tttctctggg tcgccgttac
      361 ggcgatggtc atcggatacg ctctcgccgt gcgcggggcc gcagtgtcag tcaagatcgc
      421 ggaagcggcc gtcgtctgga atcccgaaac cgggttcgcg cgctacggga aaggacgctg
      481 ccagatggcg ctcgccggtt tcaccaacat gatcgacaag gtgacaggca gggtccccgc
      541 agtctacgtc gaggggcagg aaccggctga cctcgtgtcg gtgatctatt tcggcaacac
      601 gaaagcggcg cgggcagccg gatgcgacgc ccaagagctt cgccgcggcg attggcggat
      661 caaatccgtt cccggcaagg tcttcatctt cgccaattcg ggaatggggc tcgcgtatgc
      721 cgcggccgat ttcgccgagc gccattgcgg ctactactac ctgacgccgg aaggggacga
      781 tccgtacgat ttcaatccgg cgcttacgat tccggtcggg gacatcacgg tcaagcccgc
      841 gatctacaac gccaaagtct ataccgcagt cggcggaaca tggaaccggt gggcgataaa
      901 ccggcggctg tcctccacgg acgagatgga ggggcagcac cgcgattcct atgaggtcag
      961 agacttcacg ggacggacac gtacctgcca tacgtcgttc tgttacttgc cgccggagaa
     1021 acacttcaaa gaccacccgg aatactactc gttgatcaaa gggaagcgcc agtacacgcc
     1081 ggtcggacag ctctgcatgt ccaacgagga ggtgaagcgg ctctgcctcg aacaactcct
     1141 cacgttcatt gccgaggacc gccgaaaata tccggacaac tatccgcttg tctacgattt
     1201 cacccagcag gacaacatga gtttcatgtg cgagtgcccg gagtgcaaga agatcatcga
     1261 caaatacaag cgcacgtccg gcgacaagaa ccgcaccgac aattactggg gcggtgacgc
     1321 gggattgcag cttgagttca tcaactggat cgcggacagg gttgcggaga agtatccgga
     1381 tgtcctcatc cgcacctttg cctacgtctc gaccgagacg ccaccgaaac ccggcacgat
     1441 caagccgtcg ccgaacgtct gcatctggtg gtgcgacctc tacggcgaat ccgaacacct
     1501 gcggccgatt accgccaagc cgttcaacta taaacgcggc gagcagatcc gcgagtggct
     1561 ccagctcgcg aagtctgtgc aggtttggga ctacatgctc tacggcgggt cgcattcggg
     1621 cgattttccg gaattcgcgc cggacgcgat caaggcagac gcgaagttct tcgcggaaaa
     1681 cggtttggac gacctgttca tggagtccga gcgcagcggc atcatgccgc agccgttcta
     1741 cgggcttaac tactacctga tgtcaaagct ctacacggat ccggatgaag acgtggacgc
     1801 gctcatcaaa acctactgcc gcgtttacgg caagggcgcc gcgaagatgt atgaggcgct
     1861 gcagttcctc cgccggattg agattgagaa ccctgcccaa acgcccggcg aatggcatca
     1921 gcgtgtcctc ccgtggcgga cccgtgcgaa cggggaggcg ttccgcgcgc tcgtgaagtc
     1981 cgcttacgat caggaaggcc cgggtgccgc ccgcgcccgt atggcacgcg tcctttcctc
     2041 ggtgtcgcgc gatcttgtcc gtaccttagg ccagatcccc tcggccgcgg cggagttgaa
     2101 aacggcgaag aaggatttcg ccgattattc atttgaatac gcgaaaactg cgtccatgac
     2161 ggatcaggaa cgcaagaaga tgctcgccga tatccagacc gaggtggaac tctttgatct
     2221 caagttcaag aacattccga aggaagttga aggggtgccg gaggatgaga tcaagtgcgc
     2281 cgattaccgt cgtttcagcg gttccccgaa cggcaagagg atggatgatc ctgattccga
     2341 gaccggcaag gcgttcgtct ggaaatacgg caagcctgat gccgaacata tgggattccc
     2401 ggtatcgtgc ggagtttatg acggcgcgcg caagaagaac tgcgccttca agatcacagc
     2461 cgaaatggcc ggtacggatg agcagtacca ctggatcaag ctcggcaagg gctgggtggg
     2521 cccgcagggt atgttctgga taccccacga ctggcacatg agtttccgtt ttgccgactg
     2581 gcacatcctg tgtgacggtc ttccgtcaga ccccaactgg tacgacgtct acctttccgc
     2641 caaattcacc ggccccgcct atgttcccgg ctcaacgcgt ccgaacggca tctggatcga
     2701 ccgtctcctc ctccgccgca cgcagccacc gaaggaaaac attgtgtctc cagcgaaacc
     2761 gggacgggcg gactggatgc gcggcaaata cggcttgatg gtccactggc tctttccgtc
     2821 gaaccgtgac attgaccgct ggacggacgc gtttaacgtc gatgcgtttc tgaaggactt
     2881 tgacaagacc ggtgccgaat ggctcatctt taccgctggc cagtgccgcg gcgcctattg
     2941 ctcgccgagt gaaacattca cgaaattctg cggacccggt catgtgccta agcgtgacct
     3001 gctcgccgaa atcgcctgcg gcgtgaaggc gcgcggcaaa cggttcatcg tctacgccgc
     3061 ctgtgatttt gtgaaggacg gatgtgatga ccattctgtg cagaaaggac tctgctggga
     3121 caactccact gaagaccgca ccgaatttga acggcgctgg cccctggtgc tgaaagaatg
     3181 ggcgatccgg ctcgaaaaaa actgtgacgg ctggtggctc gacggtctcg gggtacaata
     3241 tccgaacggc gtcaactgga agctgtggtg cgaggcctgc cgcgccggca accctgcggc
     3301 ggcgatcgcg ttcaactccg gcgcgaacaa tttttccgcc tacggtccgt gcgactatac
     3361 ggccggcgaa gcgagcgcgt tgaagttcgc gcttccggat ctcgatacgg ctccccccgc
     3421 cgctgcggtc cgtcaccacc ttttcccgat cgacgggtac tggggagggt tctggacctg
     3481 gcccacggcc tcgtgggccg gcgtccagca cctgatgccg acacgtcccg agctttttga
     3541 caaggcggcg atggacgcgc ggcttgagaa acgcgatttc ccggatccga tctacgacgt
     3601 ggcaacgctc ggtgcttacc tccgcaaggc aagggagacc gggtcggccg ccaccattaa
     3661 catcggcatc tctccggaag gtcggctcaa tccgaaatca atcgaactca tccgcgccgc
     3721 gcggtaaaga aagacggcat ctgggagcat tgataagtgc gatgagcaac gattgtttgt
     3781 tttcttgcag ggtgctttca aagatcgcaa ggtcgtttat ctgtgcgcat tctccagccc
     3841 catttttgta atatcaatcg agccaatgct gatcggtttc gccgttttcc atgccccgcg
     3901 ggtaaagtca ggcaattcaa caacttcccc gccacgccga tcactatcag cggaacacgg
     3961 cacaatggaa ctccaactgg cgagatcgta aacgtccatg tccagcggga gtccattctg
     4021 caagcaaaac acccaacgaa gatccatcat gaaatccatg ccgccatgtc cgccgatttt
     4081 ctttgctatc tcacccgcgt ccttatagag gggatgcata tgcagttttc gtacattctc
     4141 aagtttcttt ttgtcgaacc actcatgccc cttctgtcca cccggctcgt cactgtaagt
     4201 aatgcggaga ggatagtcat aaaagcaccc cttggtgccc tgtatcaaat tgatgcggct
     4261 gtaaggacgg ggtgttgaca tgtcgatctg catcataatc gtacgcccca aagctgtccg
     4321 aatgatcgac gtattcatgt cccccgtccg ccactcgata ttgttctgcc aatcatcggg
     4381 cttataaaaa tgccgcgcaa actcacgatg accggcagac agcgacgaca tcgaaacgac
     4441 cttctccatc cggtcgccac gattcatgtc catataggtg caaatcggtc cgagagcatg
     4501 tgtcgggtat gagtttccat atgtatgaac gaccgtgccc ggtttttgat gatgccgatt
     4561 ccggaaatga ttctccaagt gacgccacgt taaaaggtga atatacccgc cctcggcatg
     4621 tgttagcgtt cccaagagac cttgatggca caggttccat gccagcagct cattctcacc
     4681 atatacgcag ttctccaaca tcatgcaatg cacgcgattg cgctcgcttg cctcgacaat
     4741 cgcccagcag tcatcaaggg tcttggcagc aggaacctcg accatgacat gcttgcctgc
     4801 gttcatcgca tacactgcca tgcgtgcatg aagatccgct ggcgtgcaaa gatacacgat
     4861 gtcgacagca gcatcgtcac acagtccctt ccaactgtca tccttcccgg cgtaagtgtg
     4921 caacgttccg gggcttttct ttttattcaa atattctatc agtggcggaa tctgctgggg
     4981 acgcaaatcg caaagagcag ccgtctcagt accggggatg ttgctgatgc gtttaacagc
     5041 ccacgtgccg cgctcaccaa cacctacaaa accgatccga accttagtta tcggaggaac
     5101 ggtaaaattt gcaacggatg gatattcgcc aaaaggccga ttgtttacac acccagccgc
     5161 cgccgcgctc aacatccacg ccgtgccctt taagaaatca cgtcgattcc catcgctcat
     5221 tggaactgct cctgcattaa aagaacggaa acattccggc tcccctgatt gatgcctatc
     5281 atgcgggcaa ggggtgaaaa gagtcaaccc taaaaaatcg ataatccgcg cgacagcatg
     5341 gccaccctag cattccccgc gctgtcgcgt taaacaatcc catctggcgt ttcctcatcg
     5401 caggcgatgg cgctggatca cctcgtcctg ccagcgtttc gagagtgcca cgaagcgtgc
     5461 agagaacccg cataccttga cgatgagata ctgatagcgc tcgggatgct tctgcgcttc
     5521 aagaagttgc tccaccgagt tgcagttagg ttgcagcagg tggccgcact ttttcgcgta
     5581 cacgcggaac acggcggcga acgtctccgg actcatggcg gtcttgtcga acgtgaggtt
     5641 ggcgttggaa gcgaacaggc actcatgcgg cagcgtgccg atggcgttga gcacagtggt
     5701 cacgccctcc ttgcagcggt attcgcttgg agagaagccc tgcgccagac ggtcaccgtc
     5761 cctgcgtccg tcaggggtcg ccttggtggc gttgccccag tacatgaact cgcggtaggt
     5821 gtagatggcc agccggtagg cgtcgccctg atcgttggca aacccttcga tgtcgtcatg
     5881 aatccgtcgt atccaccaag ccatctcgcc gcatgtctgc tccgtgccgt cgccccagta
     5941 cggtccggcg aacgccgcct tgcggagttc cgcgccgcgc ggaccggacc agttgctccg
     6001 taccgccgcg agcagttccg gcaacgtgca ggttttcgtg tcgaagcaga ggtgcttgat
     6061 cgcgaggagc gagtcgacca cgttgccgat gaagccgagc gtgatcacac gcggacgcgc
     6121 ggggatgccg ccttcggtgg tgtcgcggcg gctctggata cccccccgca gacacatcga
     6181 gtagaccggg tgcggaaaga ccttggcgtt ctgtgcgccg taacgcgtgt actcgctcat
     6241 cacgtcgcgg aagaaacgga tgaagttgcg ataagcgatg ttgcgcgcct cttcaaagct
     6301 cgttacgcca tcgaggggat cgatgcggat cctggcttcc cgctctacct tcggatcgcg
     6361 gtggatcgcc agctcgaagg tgcgcatgac cgaaacgtag ttggcgccat ccacatccat
     6421 gacggagtca atgtagccgt tccagcagcc ggcaccggtg tactcgcggg cgtcctccac
     6481 gggaaaacct tgcctgatat actgggggat gtagcggtcg tcattcagga gcgcaaagac
     6541 ggcgtgaccc ttggtgagca tgccgccgat cttggccaga tactcttgcg gcgagtccga
     6601 agcgatgcgg cagtggagct tcgggaagac gcagtccgta ccgatgtgcg catcgagaaa
     6661 gaggcgcgtc aactcgttgt agaccggctt gccgtgcttg tcgcaaccgc cgagcgacat
     6721 cggaatctcc atctcgtggt cggagtagga gtcgacggga atcatgccat cgtgatggca
     6781 gtcggcggtg atcagaaagc gggccaccag atccgcagcc tccgcttcgg tgaggcgtcc
     6841 ggcggcaagg tcggcgcgat agagatcgta cagccatgcg tcgggacgcc ccaacgcata
     6901 gatcatcagg ccatcggtat aacccaggat ctcgcggatg aaccagagcg tgttgagccc
     6961 ttcgaaaaat gtctgcggcg gctcccaggg gcagcgttgc gcgctctcgg cgatgcgcgt
     7021 gaagtggcgg cggccggtgg catcaagcgc gggatcttcc agtcgcttct gcgcctcgcg
     7081 ggcgaaggca agttggaggt cgcgcagggt gtcgaatccg accagcgccg tttcaagcaa
     7141 cttccgtccg tacggatcgt tcttggggca cgtctccaac gcggccgcaa catcggcgcg
     7201 gatgccgccg aaacccttgg cgaggacgac gcggatcggc ggcacatggt gcatgtcgtc
     7261 cacgaacggc ccgcaacaga gtgccatgcg ttcgccgttg cgttttctga gcaggcggaa
     7321 cgcctcgtca gggatcaggt tcttctcttt gtagaagcgc ccgcatattg cgttcaccac
     7381 gcgggccggg cgcgccccgc tccagccgcc gttgacgcca gcctcgaaat agaacggcga
     7441 ctcggtgaaa aggaacggca cgaagtggcg cgtcatcgcc cggtagcttt cgcgccggat
     7501 atcgagcgcg tcgaacccag ggttcgcgtt cgcccaggcc cgcacctcgg cagcgatctt
     7561 cttttcactt tccggctggc gtggatcctt cgccacgccg cggcattcgt caggatagaa
     7621 cgcccgcaat tgctttcgca gtgccgtata cgccgcagaa tcgaaaggtg tcacgggggg
     7681 gacgttctca ccccgcaacc ccgcagccgc ggtgaacatt gcggcgctac ccaaaaaggc
     7741 gcgtcgtgtg atgttcatca tcgtcacact cctctctccc aataaaccca tattgtttat
     7801 gagggggtgg gaaaagtcaa tcccgcctgc gaaaaagagc atgtgcgcat caccctttta
     7861 cacaa
//
LOCUS       DAOSLE010000127         7634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8842_length_7634_cov_7.788890, whole genome shotgun sequence.
ACCESSION   DAOSLE010000127 DAOSLE010000000
VERSION     DAOSLE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7634
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8842_length_7634_cov_7.788890"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..3194)
                     /locus_tag="PK576_08445"
     CDS             complement(120..3194)
                     /locus_tag="PK576_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61659.1"
                     /translation="MSNFRGKDGWIGVFVCVIVLCVCCETLAALKYEPSCYVQDGLVA
                     HFDGLRNAGMLQAHNGIATNWVDLSANGNDAILKWDTVNGSRWATDGFTFEKGSYFEQ
                     LSDLTFTNTITIQVVCEVDTNLLSTSWPQLVGAGDSDRCNIYVSVGSGKRLTFKNANT
                     GNTYIPGGAWEGRFATAIRNGTQNILSQTGDGSEGATVTTSGGNIGTHTWRIGAASSV
                     LSTRLINYLTGKIQAVRIYDRVLTTEELLTNRALDDARYKTGLPVTNVIVATSVRGIE
                     GTESAGVYAVDGSHTFTAPESVTKGADTYTCSGYTLETWDAGNAAWGDPVTSAERAYT
                     ASDSGKVRLIWTWTHTAGPGALDVDAYSQDGLAMFFDGFRNAGRDVPHDSSATKWANL
                     AVNANDATLYVEDPVSRWRGDGFYFGGASYGQLDANQRVETAYTIQVVCDVDTATLKK
                     RHHDDPDRLLKWPHLIGAGSADNCNVFYRVEDADSPICFKDTASNKRYDLYAWLGDDI
                     TLRRDGCLYQGLLSHADGNWVTASINNLPVGSRTWYIATAEGSGSSAKAARFLTGIIH
                     AIRMYDRVLTDEEIAEHRVLDNARFWGRPTSNGMVVVASRIAGLEGVEPNGTYRPASH
                     TFSAPTGIQTLDGREYQADGYVVETWDAARETWITPEAGTGHTWTSPSGVDWASRRLT
                     WKWKLVRGLVTYDVGDYVQEGLIVHFDGIRNVGADLPHDSTAGSWQDLSPRRSRGLII
                     QRMPDTSGWLENGYSFAGSNYVQVAKKVSPGSEVTLEMLLDVKPSEQKGKYPTFFSGY
                     ENDSFVFFIRDTGTTLEWKQDVFAGTGYNNRPKATSWQGKDFVGVMSATHSTLYENGV
                     KKSEIGRSKVVTYDPAFWRLMANAHNWVASSPPNYNGQAIGKVFTARFYNRALTDAER
                     AWNRKVDQARYEGILPVTNVVVAVGKYATTTEAPGCYEVAGSWSFTASDATEEGKTHR
                     VSGYTLEAWANDAWSAPTSHTGASYTYVQGESPAMVRLTWRWTSGGTCVLIQ"
     gene            complement(3413..4294)
                     /locus_tag="PK576_08450"
     CDS             complement(3413..4294)
                     /locus_tag="PK576_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOE61660.1"
                     /translation="MRACIECLPCLARNAIDLARKSAQGDPDLEQRIAAGGMRILAEV
                     AETGYPLPPPCYARKLIDHALLVGDGAVPDPWAPEKKISTELALKLTTKLDEIPGWAP
                     DSFESRLRLSVAGNILDFAIYADLDMADAMETIREAFTKPLDTATVAKLKEKMDAAEN
                     IFWIFDNCGEAVFDRLLIEPYRKKMTLAVRGKPAFNDITVAELEESGYGPDFARAVVS
                     NDDGVPGVVPETCGQTFAEAFAASDLVIAKGQANFETLSDRKDHPIAFLFLAKCPVVV
                     REVNATPRSIQVILRNM"
     gene            complement(4291..5298)
                     /locus_tag="PK576_08455"
     CDS             complement(4291..5298)
                     /locus_tag="PK576_08455"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOE61661.1"
                     /translation="MKALVLEANARLVCREHELRVVKGWYRLKIAAAGICGSDLGRGF
                     NSSAYHYPLVMGHEFSAVVEEAPQGGRFAPGARVAVYPLLPCGTCAPCQKGHIQLCLN
                     YDYFGSRRDGAFAQALYAPESHLLPIPDDVSLEEAALCEPAAVAYHGVSMHSVAPGRT
                     ALVIGGGPIGLIAAQWLRIKGCTSIAVADIEEAKLDFAATFGFTAIDSRKLDDFADAF
                     DYVAEACGLAITRRAAIACCARKGHVFFIGNPADDWTLEKAFFSSILRREISFTGAWN
                     SVPDPDWKTVLAHAGKDLDLKPLITGRTSLEDAPAMFDRLIRHEGFHCKTLILPNGDI
                     E"
     gene            complement(5302..6168)
                     /locus_tag="PK576_08460"
     CDS             complement(5302..6168)
                     /locus_tag="PK576_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOE61662.1"
                     /translation="MKHGIYYAYWEDQWQADYFKYVEKVAKLGFDCLEIACSPLPGYT
                     RQQLIDLKNAASDHGIFLTAGHGPAADQNLASSDPAIQKNAKAFFIDLLKRLEILDIH
                     TIGGGIYSYWPVDYSKPIDKPGDWARSVANVREVGKVAADCGVDYCLEVLNRFEGYLL
                     NTAAEGVKFVREVDVPSVKVMLDTFHMNIEEDSIGGAIRLTKGLLGHFHTGECNRRVP
                     GRGRTPWAEIGAALKEIGYDGCVVMEPFVRMGGKVGEDIKVWRELEPGITEEKMDADA
                     KEALEFQRKVLG"
     gene            complement(6165..7535)
                     /locus_tag="PK576_08465"
     CDS             complement(6165..7535)
                     /locus_tag="PK576_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE61663.1"
                     /translation="MKSIEQRFRRGQMRMLVTTIIGYALFYFLRKNLSIAMPGLAQDY
                     GITKTSLGLFLTLHGVVYGLGKFVNGPLSDRCNSRRFLCGGLLATIVCNVIFGFGPVF
                     AALIAGDNTGTAFTTALVTVLGVAWVANGFFQSMGNPPCLRLIAHWIPPNELATKLSI
                     WNSSHSIGAGLVTILCGYIMGLGVLGADNVGIGMWRWCFWVPAIIAAVGLVVLIIWLP
                     DTPTSEGLPELAGTQATDSDADTGESTGPSAVRRVYLNPTMWVLGLSCFMVYLVRFAI
                     LDWGPMLLKEAKGVSLVGAGWAVACFEIAGILGTLFSGVATDRLAGGRAPRVCLFMML
                     GAAAAMAVFWFLPAHGPVLPYILALAVAGFFIYGPQAMTGTTAVNLATKRLAGTAVGF
                     TSLFSYASVLFSGWGMGFLADKTGGWTASFYAVIGAALVGAALFLALWNTKPDGYDEL
                     EKGNEK"
BASE COUNT         1551 a         2327 c         2184 g         1572 t
ORIGIN      
        1 caccgagatt gtcggtctgg ccgcgcagat tttggaggag cacttccggt caaagaaggg
       61 aaacgagagt tagggtcctc ttgatcaaca tgcaaaggcg gtatcgcttt tgatcgtcct
      121 cactggatca gaacgcaggt gccgccagag gtccaccgcc atgtgaggcg caccattgct
      181 ggagactcgc cctgtacgta cgtgtaggac gcgccggtgt gtgaagtcgg agcgctccaa
      241 gcatcgtttg cccacgcctc taacgtgtag ccggacaccc tatgcgtttt cccttcctcc
      301 gtggcatcac ttgccgtgaa cgaccaggaa cccgccacct cgtagcagcc gggggcttcg
      361 gttgtcgtgg cgtacttgcc gaccgccacc acgacgttcg tcaccggcag gatgccttcg
      421 taacgggcct gatccacctt ccggttccat gcccgctctg catccgtcaa cgctcggttg
      481 tagaaacgtg ccgtgaaaac cttgccgatg gcctggccat tgtagttcgg aggactactt
      541 gccacccagt tgtgggcatt ggccattaag cgccaaaagg ccggatcgta ggtcacgact
      601 ttggaacgcc ctatttcgct cttcttcacc ccattttcgt aaagggtcga atgcgttgcg
      661 ctcatcacgc ccacaaagtc ctttccttgc cagctcgtcg cctttggacg attattatat
      721 cctgttcccg cgaacacatc ctgcttccac tcgagcgtgg ttcccgtatc gcggatgaaa
      781 aacacgaacg aatcgttttc atatccagag aagaaggtgg gatatttgcc cttctgttcg
      841 gagggcttta cgtcgagcag catctctagc gtcacttccg aaccgggcga gacttttttc
      901 gcaacctgca cgtagttgga gcccgcaaac gagtaaccat tttccagcca gccgcttgtg
      961 tcgggcatgc gctgtatgat cagccctcga ctgcggcggg ggctgagatc ctgccaggag
     1021 cccgccgtgc tgtcatgcgg cagatccgcg cccacgttgc gaatgccgtc gaaatgcacg
     1081 atgagacctt cctgcacata atccccgacg tcgtatgtca cgaggccgcg tacgagcttc
     1141 catttccagg tcaggcggcg gctggcccag tccacgcccg agggcgacgt ccatgtgtgc
     1201 cccgtgccgg cttcgggagt gatccacgtt tcccgcgcgg catcccacgt ctcgacgacg
     1261 tagccatcgg cttggtactc ccgtccgtcc agcgtctgga tgccggtcgg cgcggagaac
     1321 gtatggctcg ccggccggta cgtaccgttg ggctcgacgc cttcaagtcc cgcgatacgc
     1381 gaggctacca ccaccatgcc attcgacgtc ggacgtcccc agaaacgcgc attgtcgagc
     1441 acgcggtgct cggcaatctc ttcgtccgtc agcacacggt cgtacatgcg gatggcgtgg
     1501 atgatacccg tcagaaaacg ggccgctttt gctgacgatc cagatccttc tgccgttgcg
     1561 atgtaccacg ttcgcgaccc aacggggaga ttatttatgg atgcggtgac ccagttgcca
     1621 tccgcatggc tcaaaagccc ctgataaagg cagccgtccc gacgcagcgt tatatcatct
     1681 cccaaccacg catagaggtc gtaacgtttg ttgctggcgg tgtccttgaa acagatcggg
     1741 ctgtcggcgt cttccacgcg gtaaaagacg ttgcagttgt cggcgcttcc cgcgccgatc
     1801 agatgcggcc acttcaaaag acgatccgga tcatcatggt ggcgtttttt cagcgtcgct
     1861 gtgtcgacgt cgcagaccac ctgaatggtg taggcggttt ccaccctctg attggcgtcc
     1921 agttgaccat aggaggcgcc gccgaagtag aagccgtctc cccgccaacg gctcaccggg
     1981 tcctccacgt agagcgtggc gtcgttcgcg ttgacggcga ggttcgccca cttcgtagcg
     2041 gaggagtcgt gcggcacatc gcggcccgcg ttgcggaatc cgtcaaagaa catcgcaagc
     2101 ccgtcctgcg aataggcgtc cacatccaat gcgcccgggc ctgctgtgtg cgtccacgtc
     2161 cagatgaggc gcaccttgcc cgagtccgag gccgtataag cgcgttcggc gctcgttacg
     2221 ggatcgcccc aagcggcatt tccggcgtcc catgtctcga gcgtgtagcc cgaacaggtg
     2281 taggtgtcgg cacccttcgt gacggattcg ggggccgtga acgtgtgcga gccgtccacc
     2341 gcatacacgc ccgccgactc cgtgccttcg ataccgcgga cggacgtggc cacgatcacg
     2401 ttcgtcacgg gcagccccgt cttgtaacgg gcgtcatcca gtgcgcgatt cgtcagcaat
     2461 tcttctgtcg tcaatacgcg gtcatagatg cgcacggcct ggattttgcc cgtcaggtaa
     2521 ttgataagac gtgtggacag cacactgctt gccgcgccga tccgccatgt gtgcgtgccg
     2581 atattgcctc cactagtggt gacagtcgcg ccctcgctgc catcgccggt ctgggacagg
     2641 atgttctgcg ttccattacg gatcgccgtg gcaaagcgcc cttcccaggc tccccccggt
     2701 atataggtgt tgccagtatt cgcgtttttg aacgtcagac gcttgcctga tccaacggac
     2761 acgtagatat tgcatctgtc gctatcgccg gctcccacga gctgcggcca cgatgtcgac
     2821 aacagattcg tgtccacctc acacaccacc tggatggtga tggtgttggt gaatgtaagg
     2881 tcgctgagct gctcgaaata gctccctttc tcaaacgtga agccgtccgt cgcccagcgg
     2941 ctgccgttga ccgtatccca tttgaggatg gcgtcattgc cgttggcgct caaatccacc
     3001 cagttcgtcg cgatgccgtt atgcgcctgg agcatgcccg cgttgcgcag accgtcgaaa
     3061 tgcgccacaa gcccatcctg aacgtaacag gacggttcgt acttaagcgc tgccagcgtc
     3121 tcacaacaga cacagagcac gatcacgcac acgaaaacac ctatccatcc atcttttcca
     3181 cggaaattgc tcattttcac cctcctcacc tcttccacct agcctagaag catatcagct
     3241 tttgcgttcc attcaaaggc acaacatccc tagtagaaaa cagagggact caaaagcacc
     3301 gacaacccac agaataacac agcagacaga ctagcatgct ctcgttagag gaacgtgttt
     3361 cctgcgttag gaagccgtta gaaactgctc gggcacgccc ctgagggcgg actcacatat
     3421 tccgcaggat tacctgaatc gagcgcggcg tggcgttgac ctcccggacg acgacggggc
     3481 atttcgcgag gaagaggaag gcgatcggat gatccttgcg gtcgctcaac gtctcgaaat
     3541 tcgcctgtcc tttcgcaatg acgagatcgc ttgcggcgaa cgcctctgcg aatgtctggc
     3601 cgcaggtctc cggcacgaca cccggcacgc cgtcgtcgtt cgaaacgacg gcacgcgcga
     3661 agtcggggcc gtatcctgac tcttccaact ctgccacagt gatgtcgtta aacgcgggct
     3721 tgccacgcac ggcaagcgtc atctttttgc gatacggctc aatcaacagc cggtcaaaga
     3781 cagcttcgcc acagttgtcg aagatccaga agatgttttc cgccgcgtcc atcttctctt
     3841 tcagcttcgc gaccgttgcc gtatcaaggg gttttgtgaa cgcctcgcgt atggtctcca
     3901 tcgcgtcggc catgtcgaga tcggcgtaga tcgcgaagtc gagaatgttg cccgcgacgg
     3961 agaggcgtag gcgactctcg aaggagtcgg gggcccagcc gggaatctca tccaacttcg
     4021 tcgtcaactt caaggcaagt tcggttgata ttttcttttc cggtgcccac ggatctggca
     4081 cagcgccatc gcccaccaga agcgcatggt cgataagctt ccgcgcatag cagggggggg
     4141 gcaacggata cccagtctcg gccacctctg ctagaatacg catgccgcca gcggcgatgc
     4201 gctgttcaag atcgggatcg ccttgtgccg actttcgtgc gaggtcaatc gcattgcggg
     4261 caaggcaagg gaggcattca atacaggctc tcattcgatg tctccatttg gcagaatcaa
     4321 tgttttacag tgaaacccct cgtgacggat aaggcggtcg aacatcgccg gggcgtcttc
     4381 gagcgaggtg cgcccggtga tgagcggttt caggtcgaga tcctttccgg cgtgtgccag
     4441 aacggttttc cagtctggat cgggtactga gttccaagcc cccgtaaacg agatctcccg
     4501 tcgcaagatg gatgagaaga acgctttttc aagcgtccag tcatccgccg gattgccgat
     4561 gaagaagacg tggcccttcc gtgcgcagca ggcgatggcc gcccggcgcg taatggcgag
     4621 gccgcaggct tcggccacat agtcgaacgc atcagcgaag tcgtcgagct ttcgtgaatc
     4681 gatcgccgtg aaaccgaatg tcgccgcaaa gtcgagtttc gcctcttcga tatctgcgac
     4741 ggcgatcgac gtgcagccct tgatgcggag ccattgcgcg gcgatgagtc caatcgggcc
     4801 gccgccaatc acgagcgcag ttcgtccggg ggcgaccgaa tgcatgctca caccgtggta
     4861 agcgaccgcc gccggttcgc acaatgccgc ctcttcgagc gacacgtcgt ccgggatcgg
     4921 aagcagatgc gattccggtg cgtagagagc ttgcgcgaag gcaccgtcgc gacgcgatcc
     4981 gaagtagtcg tagttaaggc agagttggat atgccctttc tggcaagggg cacatgtgcc
     5041 gcacggcaga agcggataga ccgcgacacg tgcgccgggc gcgaagcgtc cgccttgcgg
     5101 cgcctcttcg acaacggcag agaattcatg ccccatcacg agcgggtagt ggtaggcgct
     5161 ggagttgaat ccgcgcccga gatcgctacc gcagattcca gccgcagcga tcttaagtcg
     5221 gtaccaaccc ttgacgacac gcagttcgtg ttcacggcag acaagccgcg cattggcttc
     5281 cagaacgagt gccttcatgg gttagccgag aaccttccgc tggaactcga gtgcttcctt
     5341 cgcgtcggcg tccatttttt cttcggtgat accgggttca agctcacgcc agaccttgat
     5401 gtcctcgccg accttgccac ccatgcggac gaacggctcc atcacgacgc atccatcgta
     5461 gccgatctct ttcagcgcag cgccgatttc tgcccacggc gtgcgtccgc ggccgggtac
     5521 gcggcgattg cattcgcccg tgtggaagtg tccgagaagc cctttcgtca agcggattgc
     5581 gccgccgatt gaatcttcct cgatgttcat gtggaaggta tcgagcatca ccttgacaga
     5641 tggcacatcg acttcgcgaa cgaacttcac gccctcggca gccgtattga ggagataccc
     5701 ctcgaagcgg tttagaactt caaggcagta atcgacgccg cagtccgcgg cgaccttgcc
     5761 gacctcgcgg acatttgcaa cagagcgcgc ccagtcaccg ggcttgtcga tgggctttga
     5821 ataatcgacc ggccaatagc tgtagatgcc gccgccgatc gtgtggatgt cgagaatctc
     5881 cagacgcttc aagaggtcga tgaaaaacgc tttggcattc ttctggatgg ccggatcgga
     5941 cgaggcgaga ttctgatccg ctgcggggcc gtgtccagcc gtgagaaaga tgccatgatc
     6001 ggacgccgca ttcttgaggt cgatgagttg ctggcgggtg tagcccggca gtggcgagca
     6061 agcgatctca aggcagtcaa agccgagttt cgctaccttc tcgacgtatt tgaagtaatc
     6121 agcctgccac tggtcttccc aataggcgta atagatgccg tgtttcattt ttcgtttcct
     6181 ttctcaagtt cgtcatatcc gtcgggtttt gtattccaca aagcgagaaa gagtgccgcg
     6241 ccgacgagtg ccgcgccaat cacagcgtag aatgaagctg tccaaccgcc tgttttatcc
     6301 gcgaggaaac ccatgcccca tcccgaaaac aggacactcg cataggaaaa gagcgacgtg
     6361 aaccccaccg ccgttccggc gaggcgtttc gtcgcgaggt tcaccgccgt cgtgcccgtc
     6421 atcgcctgtg gaccgtagat gaaaaagccc gccacggcga gagcgaggat gtaggggaga
     6481 accggtccgt gtgctgggag aaaccaaaac actgccattg ccgccgccgc gccgagcatc
     6541 atgaagaggc acacgcgcgg ggctcgtcca ccagccagac gatccgtcgc cacgcccgag
     6601 aacagcgtac cgagaatgcc cgcaatctca aaacacgcga ccgcccagcc cgctcccacg
     6661 agtgaaacac ctttcgcctc tttcagaagc atgggccccc agtcgaggat tgcgaaacgg
     6721 acgaggtaca ccataaagca actgagaccg agaacccaca tcgtcgggtt caggtacaca
     6781 cggcgtacgg ccgaaggtcc cgttgattct ccggtgtccg catctgaatc ggtcgcctgt
     6841 gtgcctgcga gttcagggag accttcggag gtcggcgtat cagggagcca gatgatcagc
     6901 acgacaaggc caaccgcagc aatgatcgcg ggaacccaga agcaccagcg ccacatgccg
     6961 atcccaacgt tatccgcgcc aagcacccca agccccatga tgtaaccgca caggattgtg
     7021 acgagccccg cgccgatcga atgcgacgag ttccagatac tgagcttcgt cgcaagttcg
     7081 tttgggggta tccagtgggc gatgagccgc aggcacgggg ggttgcccat tgactggaag
     7141 aaaccgttcg ctacccacgc gacgcccagg accgtgacga gcgccgtcgt gaatgctgtg
     7201 cctgtgttgt cacccgcgat gagggccgcg aagacgggac cgaacccgaa gatgacgttg
     7261 cagacaatcg ttgcgagaag cccgccgcag aggaatcgcc gcgaattgca acggtcgctg
     7321 agtgggccgt tcacgaactt gccgagtccg tacacaacgc catgcaacgt aaggaaaagg
     7381 ccgagcgatg tctttgtgat gccgtagtcc tgcgcaagcc caggcatcgc gattgagaga
     7441 ttcttgcgca gaaagtagaa cagcgcgtag ccgatgatcg tcgtcacgag catccgcatt
     7501 tgtccgcgcc gaaaacgttg ttcgatactc ttcattacag atcttccgga atggcgctca
     7561 ctttcccttc ttcgcggcgt attcgcgttt gatctcgtcc acgactgcaa tgagtgtttc
     7621 aggatagtcc gtcg
//
LOCUS       DAOSLE010000128         7612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_8865_length_7612_cov_7.479026, whole genome shotgun sequence.
ACCESSION   DAOSLE010000128 DAOSLE010000000
VERSION     DAOSLE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8865_length_7612_cov_7.479026"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK576_08470"
     CDS             complement(<1..536)
                     /locus_tag="PK576_08470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE61664.1"
                     /translation="MLYSLITLSYNKLACTRRCLEALVRHTRSVVPWELIVVDNGSTD
                     GSREWCETGLIELGAACGVAVTVLRNHGRNNCSAARNLGVAAARGSHLVFVDNDVAPR
                     TRHWLDILRGVLESEPQAGMAGAKLVYPWPPYAIQCAGVGISQRGHVCFLGRGESRED
                     ARYTVRREVQCLISACLM"
     gene            complement(665..1843)
                     /locus_tag="PK576_08475"
     CDS             complement(665..1843)
                     /locus_tag="PK576_08475"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOE61665.1"
                     /translation="MMNMRPLTAKESQNLAVLNAAGIESVPLFLTATGYRKAICDATD
                     PMRAFLLKHSIHDYSTQRQGPDNKVIRQVHFLAENGLRFMDMAFYRPVTKKGDPRFWV
                     YGWKAFVDADNVLAFFFDNGSLCIANLSTVNLAAPAINTWLTRRIQQSERISKELLSR
                     LCELARRPLPATCSGDTAIGRAVEAALGIPINSSGNPDYKGIELKAKRERTSAKRNGL
                     FTCVPDWTLSHIKDFKTFLDRFGYQRGDDFKLYCTVSTKAPNPQGLLLSLGDADRFLW
                     ENVRKNGSDERLMVWPLSKLEDRLTTKHKETFWIKADSVRDNSGREFFYLKSVTHTRN
                     PNVPQFSRLLSDGSITLDHLIKRTPSGGVKEKGPQFKLAPDKFSELFLGEPKRYRLAE
                     "
     gene            complement(1840..3243)
                     /gene="dcm"
                     /locus_tag="PK576_08480"
     CDS             complement(1840..3243)
                     /gene="dcm"
                     /locus_tag="PK576_08480"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOE61666.1"
                     /translation="MMKEKHAYNENVPMPVKKQKKGSYIEPGTVFPHRKRYTCIDLFA
                     GCGGLSLGLEQAGFTPILFSEINLNAAETYMANRKGKEIIPVGDVYSLTDANLAVLKS
                     YWRLHRGIDDVDLVCGGPPCQGFSGIGHRRTFRLEKKDIPSNHLFQEMIRVISAVRPK
                     IFLFENVKGLLVSKWSAEGRNGEIFKQVHSEFSRIPGYSVRYELVHAKDYGVPQNRPR
                     VLMVGLRQDVFWKSEQMSFSLSREVDLAEYDSGRLELPKPSAIAHGFLPKSSGIPPTM
                     CELLSDLEDPNYFSKTETDSYPSEPQNEFQKMLRTLPNGKTLGRGDALTEQEYSKHRP
                     EIRKKFEYMIANNGEIPAAMRTKKFAQRVLPKTWPDNGPNITACSLPEDYVHYCQPRG
                     PTVREWARIQTFPDWYIFTGPRTTGGRRRAGDPSLGIWTRDVPRYTQIGNAVPVLLAQ
                     KVGEHLVKLLNREVIPS"
     gene            complement(3240..4214)
                     /locus_tag="PK576_08485"
     CDS             complement(3240..4214)
                     /locus_tag="PK576_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOE61667.1"
                     /translation="MRTVTFTQILRETLAAEMRRDASVILIGEDIGVYGGSFGVTRGL
                     LDEFGPERVRDTPISEQAITGVAIGAAAAGLRPVLEIMFMDFITLAVDQLVNMAAKLY
                     PIYGVRCPLVVRTPPGAGRGYGATHSQSLERLFTGVPGIKIVAPSDATDAAGLLQTAI
                     RDDNPVLFLEHKLLYPQRFEMEDGLPPPIPFGTSRFVREGTDVTLVAWSYMTQVARQA
                     AAALAEEGIEAEVVDLRTLNPLDLGIAVESAQNTGRVILVEEGPQTGGFAAEIAARLA
                     ERAHGALTCPVRRLAAPDWPVPSAKNLEAVWMPSADAIAALARSMVDA"
     gene            complement(4238..5182)
                     /locus_tag="PK576_08490"
     CDS             complement(4238..5182)
                     /locus_tag="PK576_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOE61668.1"
                     /translation="MDALSLYDLMLTIRFFEEAIERLFLEGRIMGTAHTCIGQEAVAV
                     GVAAALEPCDALTTTHRGHGHFLARGADPGRALAELFGRAVGYSRGLGGSQMMMDVSR
                     GFYGANGITGGSIPFATGLALEAQYRKTGRVTACLFGDGASNQGVFHESLNLAALWKL
                     PVLYIVENNGYAMSTATARGLADPCVAGRAAAYGIPGERVDGNDVLAVRDIVARHAAR
                     ARAGKGPSLIECVTYRLSGHSRGDPRVYRSREEEAQAWSRDPIRLFEARLLAEGVLGE
                     ADVAARHEAAQQRIETAIAFCEAAELPDPEILGEPVFA"
     gene            complement(5327..5962)
                     /locus_tag="PK576_08495"
     CDS             complement(5327..5962)
                     /locus_tag="PK576_08495"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOE61669.1"
                     /translation="MKILITAGPTREHLDPVRFLSNRSSGRMGFALARAAAERGHEVT
                     LIAGPVALPTPTGVTRTDVVSAREMLAAVERLLPAHDALVMSAAVADWRPKRNSPAKL
                     KKAAMTPVLELEPNPDILAAVRDLKGKRLFVGFAAETGDPLGEARRKLEAKGLDLIVA
                     NDVTLPDAGFEVETNRVTLIAKNAAPVPLPLLSKYEVGLRIVTWLESACSL"
     gene            6082..7449
                     /locus_tag="PK576_08500"
     CDS             6082..7449
                     /locus_tag="PK576_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61670.1"
                     /translation="MVRYESMRTSEQAATRSDYTPWRATTFYIPLLLQAVSQSLTYPL
                     VAAIVSHGEHGVVELAAFAQGQAVMFVIGALGSGLLTTGMVFGRTMEGFRTFTRINFA
                     FCCVLLTLQALTCVHPFDTFVFREILGLDPAMAGTARAVLFYSIPLHCLFLLRIPGLV
                     ALYNARASIVANWGTLCRIVLTACLSPLFVRLGWVGHEMGVLAMTGPVMAEYGLINWM
                     AKPYIRRLEPSAGQPEDSRTQFLFTVPLSFGGVLLAMAGFMIGAFISRAAEPARMLPI
                     HYVTIGIVNPVGFAALRMQAVVLAFPPRDRHDNSVFRFALAAGCMLALFPLIGQIPAV
                     AQWYFGKVQNVPACDIPLAMRAMLYMSVLPVIQSLRGHAEGLAAWRKRPNAILAGQAV
                     NLATLVCTLFVTLSFGVPGYLMGVTAILASTLMTWVTIRMGLVWAEMEDAFDEPSRPV
                     RSMEG"
BASE COUNT         1516 a         2320 c         2185 g         1591 t
ORIGIN      
        1 atcatcaggc aggcgctgat caggcactgc acttcacgcc gcaccgtgta acgcgcgtcc
       61 tcccgcgact cgccccgccc gagaaaacag acatgccccc gttgcgaaat gccgaccccc
      121 gcgcattgga tcgcatacgg cggccaggga tagacgagtt tcgcaccggc catgcccgcc
      181 tgcggttcgc tctccagcac cccccgcagg atatccagcc aatgccgcgt gcgcggcgcg
      241 acatcgttgt ccacaaaaac cagatggctg ccgcgtgccg ccgccacccc tagattgcgc
      301 gcggccgaac agttgttgcg cccgtggttc cgcaacacgg taaccgccac cccgcaagcc
      361 gcccccaatt cgatcagccc ggtttcacac cactcccggc tgccgtcggt cgagccgtta
      421 tccaccacga tcagttccca cggcacgacg gaacgcgtat ggcgcaccag cgcctccaga
      481 cagcgccgcg tacaggccaa cttattgtag gaaagcgtga tgagggagta gagcatagga
      541 ttacacatat gccagataac gggttaccgt cctttgttgc ggcctaaacg tcaagttgct
      601 tcttgttggg ttgaaaaata aagcaaaaat catcgacaaa atctttctcg caaggcgttc
      661 atcgttattc cgctagtctg tagcgtttgg gttcgccaag gaagagttcg gaaaacttat
      721 ccggcgcaag tttgaactgc gggccttttt cttttacccc acccgaaggg gtacgtttga
      781 ttaggtggtc tagcgtgata ctaccgtcgg ataaaagccg cgagaactgc ggaacattgg
      841 ggttgcgcgt atgcgttacg gatttgaggt aaaagaattc acgtcccgag ttgtcgcgga
      901 cggaatcagc cttgatccaa aaggtctctt tatgtttggt agtcaggcgg tcttcaagtt
      961 tggaaagagg ccagaccatc aagcgttcgt cactaccgtt ctttcggacg ttttcccaca
     1021 ggaagcggtc cgcgtcccca agcgagagta acaacccttg cggatttggc gctttcgtgg
     1081 agacggtgca ataaagtttg aagtcatcgc cgcgctggta tccgaagcga tccaaaaaag
     1141 ttttgaagtc cttaatgtgg cttaaagtcc agtctggaac gcaagtgaaa aggccattgc
     1201 gctttgctga tgtccgctcg cgtttggcct tgagttcaat acctttataa tcaggattcc
     1261 cgctggagtt gatgggaatg cccaacgcgg cttcaacggc acgcccgatg gcggtgtcac
     1321 ccgagcaagt cgcagggagc ggtctacggg caagctcgca cagtctcgac aggagttcct
     1381 tcgagatgcg ttcgctttgc tgaatgcggc gggttaacca tgtgttgatc gcaggggcag
     1441 ccagattcac ggtcgacaga ttggcaatac agagcgaacc gttatcgaaa aagaacgcaa
     1501 gcacattgtc cgcatccaca aacgctttcc atccgtacac ccaaaatctc ggatcgcctt
     1561 tcttggtcac aggacgatag aatgccatat ccatgaaacg taggccgttc tcggcaagga
     1621 agtgaacttg tcggatgact ttgttgtccg gcccttgtct ctgtgtggaa taatcatgga
     1681 tcgaatgctt gagcagaaac gcccgcatgg ggtccgtcgc atcgcaaatc gccttacgat
     1741 atcccgtcgc ggtcaggaaa agcggaacac tttctattcc ggctgcattg agaacggcaa
     1801 ggttctgcga ctctttcgca gtaagtggac gcatattcat catgacggga taacctcccg
     1861 attaagcagc tttaccagat gttctccgac cttctgtgcg agcaacacgg gaactgcgtt
     1921 accgatttgc gtgtagcgtg gaacatcgcg cgtccagatg ccaagcgacg gatccccagc
     1981 acgtcgacgt cccccggtcg tccgtggccc cgtgaagata taccaatccg ggaaagtctg
     2041 gattcttgcc cattcacgaa cggttggccc tctcggttgg caataatgca cataatcttc
     2101 tggcaaagaa caagccgtga tgttcggccc attgtcaggc catgttttcg gcaacacgcg
     2161 ttgagcgaat ttcttggtcc gcatcgctgc ggggatttct ccgttgttcg caatcatata
     2221 ttcaaatttc tttcgtattt caggtctatg ttttgaatat tcctgttcgg tcaacgcatc
     2281 tcccctcccc aacgtttttc cattgggaag cgttcgcaac atcttttgaa attcgttttg
     2341 aggctcagac ggatacgagt ccgtttccgt ttttgagaaa taattggggt cttccaagtc
     2401 cgaaagcaat tcgcacattg tcggggggat tcctgacgat ttaggcaaaa atccgtgcgc
     2461 gatcgcgctt ggtttgggta actcaagccg ccctgaatca tattcggcta gatctacttc
     2521 tcttgaaaga gagaacgaca tctgttccga tttccaaaag acatcctgcc gcaaaccgac
     2581 catcagcaca cgggggcgat tttgcggaac accataatcc ttggcatgaa caagttcata
     2641 gcggacggaa taccctggga tccgtgagaa ctcactgtgc acttgcttga aaatttctcc
     2701 gtttcttccc tctgcagacc attttgaaac aagtaatcct ttgacattct caaaaagaaa
     2761 gattttgggc cggacagcgc tgatgacacg aatcatctcc tgaaacaaat gattcgacgg
     2821 aatgtctttt ttttcgagac ggaatgtgcg ccgatgcccg attccgctga atccttgaca
     2881 aggcgggccg ccacaaacca aatcaacatc atcgattcca cgatgtaacc tccaatagga
     2941 ttttagaacc gcaagattgg catcggtcag actatacacg tccccaacag gaatgatttc
     3001 cttgcccttg cggtttgcca tgtaggtttc ggcggcattg aggttgattt cgctaaacaa
     3061 gatcggagtg aaacccgcct gttcaagacc aagcgacaac ccgccgcatc cggcaaacaa
     3121 gtcaatacag gtgtagcgtt tgcgatgcgg aaaaaccgta cccggttcga tataagatcc
     3181 ttttttttgt tttttcacgg gcataggcac gttttcattg taagcgtgtt tttccttcat
     3241 catgcgtcga ccatgctacg agccagagcg gcaatcgcgt ctgcggacgg catccagacc
     3301 gcttcgagat tcttggctga gggcaccggc cagtccggcg cggccagacg ccgcacgggg
     3361 cacgtcaacg cgccgtgcgc ccgctcagcc agtcgcgccg caatctcggc cgcgaacccg
     3421 ccggtttggg ggccttcttc cacaaggatc acgcgacccg tgttctgcgc cgattcgacc
     3481 gcgatgccga ggtcaagcgg attaagggtg cgcagatcca ccacctccgc ctcgatcccc
     3541 tcttcagcca acgccgccgc agcctgccgc gccacctgcg tcatgtacga ccacgccacc
     3601 agcgtgacgt ccgtgccctc gcgcacgaac cgcgacgtgc cgaaggggat cggcggcggc
     3661 agcccgtcct ccatttcaaa ccgctgcgga tacagcaact tgtgctcgag gaacaacacc
     3721 gggttgtcgt cccggatcgc ggtctgcaac agtcccgccg cgtccgtcgc gtcagagggc
     3781 gccacgattt tgatgccggg cacaccggtg aacagccgtt cgagagattg cgaatgcgtc
     3841 gcgccatacc cccgccccgc gccgggcggc gtgcgaacca ccagcggaca gcgaaccccg
     3901 tagatcggat agagtttggc ggccatgttc acgagctggt ccaccgccag cgtgatgaaa
     3961 tccatgaaca tgatctcaag caccggacgc aggcccgcgg ccgcggcacc gatcgcgacc
     4021 ccggtgatcg cctgttcgga gatcggcgtg tctcgcacgc gctcgggccc gaactcatcg
     4081 agtagaccgc gcgtgacgcc gaaggaaccg ccgtatacgc cgatatcctc gccgatcagg
     4141 atcaccgagg cgtcacgccg catctcggcg gccaacgtct cgcgcaaaat ctgggtgaac
     4201 gtgacggttc gcataggcag cacacacctc ctctctgtca ggcaaaaacc ggttcgccca
     4261 gaatctccgg atccggtagt tccgccgcct cgcaaaaggc gattgccgtt tcgatgcgct
     4321 gctgcgcggc ctcgtgccgc gcggcaacgt ccgcctcccc gagcaccccc tccgcaagca
     4381 gacgcgcttc gaaaagacgg atcggatcac gactccaggc ctgggcctct tcctcccggc
     4441 tgcggtacac gcgcggatcc ccgcgcgaat ggcccgaaag ccggtaggta acgcactcga
     4501 tcagggacgg ccccttgccg gcgcgcgccc gcgccgcatg acgcgccacg atgtcgcgca
     4561 ccgccagcac gtcgttcccg tccacccgtt cgccgggtat accgtaggcc gccgcgcggc
     4621 ccgccacaca gggatccgca agcccgcgcg ccgtggcggt tgacatcgcg tagccgttgt
     4681 tctccacaat atacagcacc ggcaacttcc acagcgccgc gaggttcagc gactcgtgaa
     4741 acacgccctg attggaggcg ccgtccccga aaaggcaggc cgtcacccgt cccgtcttgc
     4801 ggtattgcgc ttcgagcgcg agcccggtgg cgaatgggat gctgccgccg gtaatgccgt
     4861 tcgcaccgta gaacccccgc gacacgtcca tcatcatctg gctgccgccg aggccgcgcg
     4921 aatatccgac ggcgcggccg aagagttcgg ccaaggcccg accgggatcc gcaccgcgcg
     4981 ccagaaaatg tccgtgcccg cggtgggtcg tggtcagggc gtcgcacggc tccagcgcgg
     5041 cagccacccc caccgccacc gcctcctgcc cgatgcaggt gtgcgccgta cccatgatgc
     5101 gcccttccag aaacaggcgc tcaatcgctt cctcgaagaa gcggatcgtc aacatcagat
     5161 catatagcga aagcgcgtcc atgccccccc tgctgttgct tctccctcaa aatggtctgc
     5221 caagtatagc acacttgctc catcagggca aaccgtcgcg agaccccggt ttgtttcggg
     5281 ttgccggctc agaccgatca tccaaatcgg aggcgtccca accgggttac agggagcagg
     5341 ccgattccag ccacgtaacg atccgcaggc cgacctcata tttgctcaga agcggcaagg
     5401 gaaccggcgc ggcgtttttc gcgatcagtg tgacgcggtt ggtttcaacc tcgaaccctg
     5461 catccggcaa ggtcacgtca ttggccacaa tcagatccag ccctttggct tccagtttgc
     5521 gccgggcttc gcccaaaggg tcgccggttt cagccgcgaa tccgacaaag agtcgtttcc
     5581 ctttgaggtc gcgcactgcc gcgaggatat ccggattcgg ttccagttcg agcaccggcg
     5641 tcatggcggc tttcttgagt ttcgcggggc tgttccgttt gggacgccaa tccgccacgg
     5701 ccgcagacat caccagcgcg tcgtgcgcgg gcagaagccg ctccacagca gcgagcatct
     5761 cgcgcgcgct gaccacatcc gtgcgggtca ctccggtcgg ggtgggcaac gcgaccggtc
     5821 cggcgatcag ggtgacctca tgcccgcgtt ccgctgcagc ccgcgcaagg gcgaagccca
     5881 tcctgccgct cgaccggttg ctgagaaaac gcacggggtc gaggtgctcg cgtgtggggc
     5941 ctgctgtgat caggattttc atcgtttcgg atgctcgcca gtataacaga aagaaacaga
     6001 accggggcat acccattttg gcgaatcgtt ccgagaggca catccccttg gttcgatgag
     6061 cattggattt ccggggcggg catggtaaga tatgaatcca tgcggacatc cgagcaggct
     6121 gcgacccgat ccgactacac accgtggcgg gccaccacat tctacatccc gcttttactt
     6181 caggcggtct cgcagagcct gacctatccg ctggtcgcgg cgatcgtgtc gcacggcgaa
     6241 catggggtcg tggaactggc cgcattcgcg caggggcagg cggtgatgtt tgtgatcggc
     6301 gcgttgggca gcggcctgct gaccaccggg atggtgttcg gaaggacgat ggaagggttc
     6361 cgcaccttca cgcgcatcaa cttcgcgttc tgttgcgtgc tgctgacgct gcaagcgctg
     6421 acatgcgtgc atccgttcga cacctttgtt ttccgggaga ttctggggct ggatccggcg
     6481 atggccggca cggcccgcgc ggtcctgttc tacagtatcc cgctgcactg ccttttcctg
     6541 ctgcgcattc cggggttggt ggcgctttac aacgcgcgcg cgagtatcgt ggcgaactgg
     6601 ggaaccctgt gccggatcgt actgacggca tgcctgtcgc cactgttcgt gcgactaggc
     6661 tgggtgggac atgagatggg ggttctggcc atgaccgggc cggtgatggc ggaatacggg
     6721 ctgatcaact ggatggcgaa accgtatatc cgcagattgg aaccgtccgc cgggcagccg
     6781 gaggattccc ggacgcagtt cctgtttacg gtcccgctct cgttcggcgg ggtgctgctg
     6841 gccatggcgg gcttcatgat cggcgcgttt atctcgcgcg cggcggaacc cgcgcgcatg
     6901 ctgccgattc actatgtcac catcgggatc gtcaatccgg tggggttcgc cgcattgcgc
     6961 atgcaggcgg tggtgctggc ctttccgccg cgtgaccggc atgacaactc ggtcttccgg
     7021 tttgcgctgg ccgcaggctg catgttggcg ttattccctc tcatcggaca aatcccggct
     7081 gtggcgcagt ggtatttcgg gaaggtccag aatgtgccgg cgtgtgacat tccgctcgcc
     7141 atgcgcgcca tgctgtacat gtcggttctg ccggtgatcc agtcgttgcg cggacatgcc
     7201 gaggggttgg cggcttggcg taagcggccg aatgcgattc tggccggaca ggcggtcaat
     7261 ctggccacgc tggtctgcac gctttttgtg accctgagct tcggcgtgcc gggatacctg
     7321 atgggggtga cggcgatcct tgcgtccacg ctcatgacat gggtgacgat ccggatgggg
     7381 ttggtttggg cggagatgga ggatgccttt gacgaaccct cgcgcccggt gcgcagtatg
     7441 gagggataag cccgccgccc gcagagcagg cacaaacgct gggacaacct gatccacaga
     7501 ttaccctaaa aggagcagtt gaacgggtag aattccagca aagccccgca aacattgggt
     7561 tttgcggtgc gcccgacgct ctttttgaac tcttggtccc tgtcgatcgt cg
//
LOCUS       DAOSLE010000129         7468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9078_length_7468_cov_7.572373, whole genome shotgun sequence.
ACCESSION   DAOSLE010000129 DAOSLE010000000
VERSION     DAOSLE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9078_length_7468_cov_7.572373"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PK576_08505"
     CDS             <1..293
                     /locus_tag="PK576_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61671.1"
                     /translation="AMIAVAIPNIGTWVKNPVLYEIGQTTVSPGTYNVIQGMSAIEKG
                     RYLVNAAGGSYVRAAFGTAWRQTGTTIGTGLTPGGYLLGLGIFETVDYQTRK"
     gene            304..486
                     /locus_tag="PK576_08510"
     CDS             304..486
                     /locus_tag="PK576_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61672.1"
                     /translation="MNIFELLTVIGVIVVGYFCGKYFGLHYGLGGWIGGFVLGVLLAI
                     AAYCIFRRLIGVTGKK"
     gene            763..972
                     /locus_tag="PK576_08515"
     CDS             763..972
                     /locus_tag="PK576_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61673.1"
                     /translation="MKQPLNIGISTEELNVADSILKATVNAFHTGSAAPRPAERDASG
                     RWIAPNVPMHPMSNGLVRIQFRVGQ"
     gene            1101..5444
                     /locus_tag="PK576_08520"
     CDS             1101..5444
                     /locus_tag="PK576_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61674.1"
                     /translation="MKKLFRQRVVLIAVMLAAGRLCAQKMVYITQEQDCGIAPIPAEL
                     NEWRTGAMTLRDPKPTPADVVVTGDDFFTREYGAPFSVEFNTGTNGWTRIFGTRGCQP
                     KEVSIRFGRGWGRQEGRKFQLQMDIEQSVAETEWDWAMWENGTRHKVPFKLTTSAGKR
                     SRVVQDLGVVRMPQRFSGVELTCRTKNVHVKILSAKIVPVTVPMTWRGIFVLGFDPWR
                     AGMTLARRNNYTLSINGTELSRGLSIDGGETLKMEIGEHLKKGTNIIEVVTEYHSGYA
                     DPGALLTECFAVSATGETTFLLAGPDWRVRVGDGPWLNPHYSRNPKSRAGFETLPSGK
                     QVATGNQPMHGGPLQVCHLGPKFPVFDCEDKTVEWQVSLPPRMTEATVACVVSNAITG
                     EVAERLSRSVAPQAGQHSTTAIFRLASRETGAYKLYWTLARGADVLDRVRTEMIVAGP
                     VEQTAVPFNEFDNLLESRMKVIQTRDCTVDELNPTNFAQYRGSAVVGEFPAEGLSRVG
                     REGGLVFRETGERGGDWFGYKLDVEHLGRAHILEVDYPDTREQLLYVRVCESYPIHFC
                     NNTARGGSGHANASGAVRTGWMEPLTGKKEKLRILFYPGSKNITVTFENGGLGQTRAA
                     VCGFRLYEVPEGVPALELPPTDRLYGNHCERPLCGMWGASVASRFHYSWTEAFENVYA
                     AAFAAAANRIRFLRYSGQNLAIEGVYMYKQGFPTLSGESYLGVESFDFNYVLSKMYRR
                     NGIRAFACFEYIASPAIMLGGGYDIGDSEMRAAKGPLAPSYSVSRYGKQVVGFGGMGL
                     NYLAPHVWRSITNLVTEIYGRYEPTGGFEGLFMINNGWWIPGFPAASGDRLSEREVGY
                     DDLSVTLFEKDTGISLGIPYTPERFEKRYEALNSKHAQAWYAWRARKMRESLETIGDL
                     VAKGENKWAVYASPGIRRTREDSPFDSIFSTAEERDTDYPRLLAEAGFPSDLYGRGTD
                     AWVTLVPRVAFARAHDLKHWGAAMNAGARAIYAKQDAVYFDPTGLNERWTSTSGLDAW
                     WWRAAGSTVFDVKPSGTGAFFDMVDVCVDHTPTLMIHTWLDVNYPTAHGREGRRFATG
                     FLASPVGDYTDCPSVCGVQARRCGDAVQLVNDTPYALEGVLRSKTLGLFPHTVEDAFA
                     GKKRGTFPYALEPYGITVLKGCNGSFSGEFRFADAAVAEQAMARTDALLANKLMCAKI
                     KPQLVARLQKARLEKDAYTAVATLRDFEVRRVADAFFDHVITAKNTKPASELKVIGRY
                     DGAEPKRFKAGEPGQGLGAVDAKYKSATRARTLSLLVKTGGSDCAILLHDTPLRYDLR
                     VSNGGLNAAGLVFRVKPGQKGYFSYGLKDSDSVAANEWHHLCWTVDPEGSATLWVDGD
                     LVDAVELDALHGVALDPASGGLNASRPTVPEGKTFADILIAREIFREGVMDWRDIQKE
                     AAEWLGKGETK"
     gene            5441..7309
                     /locus_tag="PK576_08525"
     CDS             5441..7309
                     /locus_tag="PK576_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOE61675.1"
                     /translation="MRRLLIFSVAAFAAATQGAVIEEIRVPSPSMGKDIPACVVLPDK
                     YAADPGMAFSTVYVVHGAGGNYRTYATEELKFLADRHEFLFVCADGGKTSWWFDSPID
                     PSYRYETHVTKELVPWIDAHYRVIRDRAHRAIMGGSMGGHGACWLGFRHKELFGAIGS
                     IYGGMNLWNFPNSWDIAKRLGPRDEFPERWREHSVVTEAMKLKNGDVNLIMAVGTSDF
                     FLSANRELHNILTSNKVAHTYIEIRTAEEKTSGHSGEFNAMVKPIITAFFDNYFKNGE
                     AQIATAVTPFVTRYASKVTKAAHGTTPAPEIKVPAPWRVVEGDEAKTGLNVREYYFTI
                     EAPGRGLAAPRVELVWPGVAVKTVYGAKNMVRDGDRVLFTPTATKPPTGFTTGWSEGA
                     IRICVFHNVEGVQHGLYRDRPYPAAEVRAQNNWLFAARELFRRAGLAETNATEGAFVQ
                     LFGFESNFPNGHVDAPPHFHVMLMWNAWKENNVGHYRLDGKGRITENAFLVCGNVPGY
                     PRTGYFPQQLGETTHYNAPSGKTLFTLEMLRDGTGLVFRRPGVPGEYLLGSPEPVSSV
                     CVSFREDGTRPWRELARVSADDDSREGVLTIRTTVDGQCSVETVRYNPDTGERIKP"
BASE COUNT         1509 a         2151 c         2357 g         1451 t
ORIGIN      
        1 acgcaatgat agcagtggca attccgaaca taggaacgtg ggtcaagaat cctgtgcttt
       61 acgaaatcgg acagactacg gtttcgcccg gtacctacaa tgtcatccaa ggcatgagtg
      121 ccattgagaa agggcgctac ttggtcaatg ccgcaggtgg tagctacgtc cgggcagctt
      181 tcggtacagc atggcgccaa actggcacaa ccatcggaac tggacttacg cctggtggct
      241 atcttctggg acttggtatc tttgagaccg tagactacca gacacgaaaa tgaggtatcg
      301 tctatgaaca tctttgaact tcttacagtg attggtgtaa tcgtggtcgg gtatttttgc
      361 ggtaagtact tcggcttgca ttacggactt ggtggctgga taggcgggtt cgttctcgga
      421 gtcctgcttg ccatcgcagc atactgcatc ttccgccgat tgatcggtgt gaccggcaaa
      481 aagtaattct aggaaagaaa agggggattc gttcgcggag catcggtgtt acttcaatga
      541 accgtaatta caaagttatc atcgctaggt tgattagcgc actgttgtgt tgtttcggtg
      601 gcggggtagc acaatatttg ttatggcatc aatctggcga accgtggtac gaggctctgt
      661 ttcgttggtc atttctttgg tttttttatg ttcttttggc atcgttagcc ggagagcaca
      721 ttccaaaaat gctgtcaaaa aacgataatg aacgccgcgc acatgaaaca accactgaac
      781 ataggcatta gtaccgaaga gctgaacgtg gctgacagca tcttgaaggc cacggtcaac
      841 gctttccaca ccggcagcgc ggccccgcgc cccgccgagc gggacgcgtc gggccgctgg
      901 atcgcgccca acgtcccgat gcaccccatg agcaacggcc tcgtccgcat ccagttccgc
      961 gtcggccaat gaaccgtgga tatgccccgc atagttgggc acggcttttt ctttttgaaa
     1021 accgaaaccg gtgttgttag tattccttcc gattttgggt cacgaagaat atccggacag
     1081 aaaacatgag ggggaggccg gtgaagaagt tgttcaggca aagggtcgtg ctgattgcag
     1141 tcatgctggc ggcggggcga ctgtgtgcgc agaagatggt ttacatcacg caggagcagg
     1201 actgcggcat tgcgccgatc ccggctgaac tgaatgagtg gaggacgggg gcgatgacgc
     1261 tccgcgaccc gaaaccgacc cctgcggacg tggtcgtcac gggtgatgat ttctttaccc
     1321 gcgaatacgg tgcgccgttc agcgttgaat tcaacacggg aacgaacggc tggacgcgga
     1381 tcttcggcac aaggggatgc cagcccaagg aggtttcgat ccgtttcgga cgcggctggg
     1441 gccgtcagga gggacgcaag ttccaactgc agatggacat cgagcagtcc gttgccgaaa
     1501 cggaatggga ctgggccatg tgggaaaacg ggacccgcca caaggtaccc ttcaagctca
     1561 caacctccgc cggaaagcgc tcacgggtgg tacaggatct tggcgtggtg cggatgcccc
     1621 agcgtttcag tggcgtggaa ctcacgtgcc gcacgaaaaa tgtccacgtc aagatcctct
     1681 ccgccaagat cgtgccggtg accgtgccga tgacctggcg cgggattttt gtgctcggct
     1741 tcgatccgtg gcgcgccggc atgacccttg cgcggcgcaa taactacacg ctctctatca
     1801 acggaacgga gctttcgcgg gggctttcca tcgacggcgg cgaaacgctc aaaatggaaa
     1861 tcggtgaaca cttgaagaag ggcacaaaca tcattgaggt cgtcacggaa tatcacagcg
     1921 gctatgccga tcccggcgcg ctgctgacgg agtgtttcgc cgtatcggcg acaggcgaga
     1981 caacgtttct gttggccggg cccgactggc gggtgcgcgt cggcgacggc ccgtggctga
     2041 accctcatta ttcgagaaac ccgaagagcc gagcggggtt cgagaccctt cccagcggca
     2101 agcaggtcgc gacaggcaac caaccgatgc acggcggccc gttgcaagtg tgccatctgg
     2161 ggccgaagtt ccccgttttc gactgtgagg acaagacggt cgagtggcag gtttcgcttc
     2221 cgccccggat gacggaggcg accgtcgcct gcgtggtgtc aaacgccatc acaggcgaag
     2281 tggcggaacg gctctcccgg tctgtcgcgc cgcaagccgg acaacattcc acgaccgcca
     2341 tttttcggct tgcctcccgc gaaaccggcg cctacaagct ctactggacg ctcgcgcgcg
     2401 gtgccgacgt gctcgaccgt gtgcgcacag agatgatcgt ggcgggacct gtggagcaaa
     2461 cggcggtacc gttcaacgaa tttgacaacc tccttgaaag ccgcatgaag gtgatccaga
     2521 cgcgcgactg cacggtggat gagcttaacc cgacaaattt tgctcagtat cgcggcagcg
     2581 cggttgtcgg cgaattcccc gccgaagggt tgtcgcgtgt cgggcgcgaa ggcgggctcg
     2641 tgtttcgcga aacgggtgag cgcggcgggg attggttcgg ctacaaactc gacgtggagc
     2701 atctcggacg ggcacacatt ctggaggtgg actaccccga cacgcgtgag cagcttctct
     2761 acgtgagggt gtgcgagtca tatccgatcc atttctgtaa caacacggcg agaggcggat
     2821 cgggacatgc gaatgcctcc ggagcggttc gcaccggatg gatggagccg ctcaccggca
     2881 aaaaagagaa gttgcgtatc ctcttttacc ctggctcgaa gaacatcacc gtcacgttcg
     2941 agaacggcgg tctgggccag acacgggcgg cggtctgcgg attccgtctg tacgaggtgc
     3001 cggagggtgt tcccgcgctc gaactaccgc caacggatcg cctctacggc aaccactgcg
     3061 agcggccgct ctgcggcatg tggggcgcgt ccgtggccag ccgcttccac tacagttgga
     3121 cggaggcgtt cgaaaatgtt tatgctgccg cgtttgccgc cgccgcgaac cgcatccgct
     3181 tcctgcgtta ttcgggacag aacctcgcga tcgaaggcgt gtacatgtat aagcagggtt
     3241 ttcccacgct ctcgggggag agctatctgg gcgtcgagtc cttcgacttc aactacgtgc
     3301 tctcgaagat gtaccggcgc aacggcatcc gcgccttcgc ctgtttcgag tacatcgctt
     3361 cgccagccat catgctgggc ggcggctatg acatcgggga cagcgagatg cgtgcggcga
     3421 aaggccccct cgcgccttcc tattcggtaa gccgctacgg caagcaggtg gttggctttg
     3481 gcggcatggg gctcaattac ctcgcaccgc acgtgtggcg gtcgatcacg aatctggtta
     3541 cggagattta tggccgttac gaaccgacgg gcggcttcga aggcctcttc atgatcaaca
     3601 acggctggtg gatccccggc tttccggccg caagcggcga cagactctcc gaacgcgagg
     3661 tgggttacga cgacctttcc gtaacgcttt tcgagaaaga tacggggatt tctctcggga
     3721 tcccctatac gcctgaacgc tttgagaaac gttatgaagc cctcaattcg aaacatgcgc
     3781 aggcgtggta cgcttggcgc gcccgaaaga tgcgcgaatc gctggaaacg atcggcgacc
     3841 ttgtcgcgaa aggcgagaac aagtgggccg tctatgcctc gcccggcatc cggcgcacgc
     3901 gggaggatag cccgttcgac tcgatctttt ccaccgcaga ggaacgggac actgactatc
     3961 cccgccttct tgcggaggcc ggtttcccgt ccgacctcta cgggcggggt acagacgcgt
     4021 gggtcacgct cgttcctcgg gtcgcgttcg cgcgagccca cgacctgaaa cactgggggg
     4081 ctgcgatgaa cgcgggagcg cgcgcgatct acgcgaagca agacgccgtt tacttcgatc
     4141 ccaccggcct gaacgagcgt tggacctcca cgtccggcct cgacgcatgg tggtggcgcg
     4201 cggccggctc gaccgtcttt gacgtaaaac cctccggtac cggcgcgttc ttcgatatgg
     4261 tggacgtttg cgtcgaccac acgccgacgc tgatgatcca cacatggctt gacgtcaact
     4321 accccaccgc gcacggacgc gagggacggc ggttcgccac cgggttcctc gcgagccctg
     4381 tcggcgatta cacggactgc ccgtcggtct gcggcgtgca ggcgcgccgt tgcggcgacg
     4441 cagtgcaact cgtgaacgac acgccgtacg ctttggaggg cgtgctgcgt tcgaagacgc
     4501 tggggctttt cccgcacacg gtcgaggacg ctttcgccgg caagaagcgc gggacgttcc
     4561 cgtacgcgct cgaaccttac ggcatcacgg tgctgaaggg atgcaacggc tcgttttcgg
     4621 gtgagttccg tttcgccgat gcggctgttg cggagcaggc catggcgcgg acggacgcgc
     4681 ttctcgcaaa caaactcatg tgcgcgaaaa tcaagccgca actcgtcgcg cggctccaaa
     4741 aggcgcggct ggaaaaggac gcctacacgg ctgtggccac gttacgcgat ttcgaggtgc
     4801 gccgagtcgc ggacgctttt ttcgatcatg tcatcaccgc gaaaaacacg aaacccgcat
     4861 ctgaactgaa agtgatcgga cgctacgacg gcgccgaacc gaaacgtttc aaggccggtg
     4921 agcccggtca gggattgggt gccgtggatg caaaatacaa gagcgccacg cgggcgcgaa
     4981 cgctatcgct gcttgtcaag accggcggct ccgactgcgc gattctgctg cacgacacac
     5041 cgctccgtta cgatctcagg gtaagcaacg gcggactgaa tgcggcgggc ctcgtctttc
     5101 gtgtcaaacc gggccagaag gggtatttct cttatgggct gaaggattcc gattcggtgg
     5161 ctgcgaacga gtggcatcac ctgtgctgga cggtcgaccc ggaggggtcg gcgacgttat
     5221 gggtggacgg cgatttggtg gatgcggtcg agcttgatgc acttcacggc gtggcactcg
     5281 accccgcgtc aggagggctc aacgcatcgc gaccgactgt cccggaaggc aaaacgtttg
     5341 cggacatcct gatcgcgcgc gaaatctttc gtgagggcgt gatggattgg cgcgacattc
     5401 agaaagaggc cgccgagtgg ctcggtaaag gagagacgaa atgaggagac ttttgatttt
     5461 ttccgtggcc gcgttcgcgg cggctacgca aggcgcggtg attgaggaga tccgggttcc
     5521 gagcccgtcg atgggcaagg acatcccggc ctgtgtggtc ttgccggaca agtacgccgc
     5581 cgatccgggc atggcgttca gtacggtcta cgtggtgcat ggcgcaggcg gcaactaccg
     5641 cacgtacgcg acggaagaac tgaagtttct ggccgaccgc cacgaattcc tgttcgtctg
     5701 cgccgacggc ggaaagacga gctggtggtt cgattcgcct atcgacccgt cgtatcgtta
     5761 cgaaacccat gtaacaaagg aactcgtgcc atggatcgat gcgcactacc gtgtcatccg
     5821 cgaccgggcg catcgcgcga tcatgggcgg atccatgggc ggccacggcg cctgctggct
     5881 cggattcaga cataaggaac tcttcggcgc gatcggcagt atctacggag gcatgaacct
     5941 gtggaatttt ccgaatagct gggacatcgc caaacgcctt ggcccgcgtg atgaattccc
     6001 cgaacgctgg cgggagcact ctgtggttac agaggccatg aaactgaaga acggcgatgt
     6061 gaacctgatc atggccgtgg gcacatcaga cttcttcctg tccgcgaacc gcgagttgca
     6121 caacatcctt acgtcgaaca aggtggcgca cacctacatc gagatccgca cggccgagga
     6181 gaagaccagc ggccactcgg gcgagttcaa cgccatggtg aagccgatca tcacggcatt
     6241 cttcgataat tactttaaga atggtgaggc gcagatcgcc acggccgtga cgccgttcgt
     6301 cacgcgctat gcctcgaagg tgacgaaggc cgcgcacggc acgactcccg cacccgagat
     6361 caaggtgccc gcaccatgga gggtggtgga aggggatgag gcgaaaacgg ggctcaacgt
     6421 gcgcgagtat tatttcacga tcgaggcccc cgggcgcggc ctcgccgcac cgcgcgtgga
     6481 actcgtctgg ccgggtgtcg cggtgaaaac cgtgtatggg gcgaagaata tggtgcgcga
     6541 cggcgaccgt gtccttttca cgcccacagc gacgaagccg cccaccggtt tcaccacggg
     6601 gtggagtgag ggtgcgatcc gcatctgcgt cttccacaat gtggaggggg ttcaacacgg
     6661 tctgtaccgc gacaggccct atcccgctgc ggaggtccgt gcgcaaaaca actggctgtt
     6721 cgccgcgcgc gaattgttcc ggcgcgccgg actcgccgag acgaacgcga ccgaaggcgc
     6781 gtttgtgcag cttttcgggt ttgagtccaa cttcccgaac ggccatgtgg acgcgccgcc
     6841 gcacttccat gtgatgctga tgtggaacgc ctggaaggaa aacaacgtgg ggcattaccg
     6901 tctcgacggg aaaggacgca tcacggagaa cgccttcctc gtctgcggga acgtgcccgg
     6961 atatccccgg accggctact tcccgcagca actgggcgag acgacgcact acaacgcccc
     7021 ctccggaaag acgctcttca cgcttgaaat gctccgggac ggtaccggtc tcgtgttccg
     7081 ccgccccggc gtgcccggcg agtacctgct cggttctccc gagcctgtct catcggtctg
     7141 cgtttcgttc cgcgaggacg gcacgcgccc ctggcgtgaa ctcgcgcgcg tgtcggcaga
     7201 cgatgactcc cgcgagggtg ttctgaccat ccggacaacg gtggacggcc aatgctcagt
     7261 cgaaaccgtc cgctataatc ccgacaccgg cgaacggata aaaccataag aggctcctgc
     7321 cccgcgaccg gcgacattag ccaaacgcaa aggttgccaa agtaatcagt ttgtgacagg
     7381 ggtgtgcgaa gacaatgcgt ttctggtgaa aaggggggca gtgagtcggg agcgcgggcg
     7441 tccccgcccg caacagcgtg tcttgcgg
//
LOCUS       DAOSLE010000130         7303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9325_length_7303_cov_7.406595, whole genome shotgun sequence.
ACCESSION   DAOSLE010000130 DAOSLE010000000
VERSION     DAOSLE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_7303_cov_7.406595"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1100)
                     /locus_tag="PK576_08530"
     CDS             complement(294..1100)
                     /locus_tag="PK576_08530"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOE61676.1"
                     /translation="MKLIRFLTPSSPTPLTGILHEDGSAEPIEGDLFATWEPTGRRIE
                     ITSLLAPVRPPLILAIGLNFRAHAAETGQPIPDFPVLFMKSPQAVLDPGAPIRIPTRL
                     PSTQVDYEAELVVIIGRTCTNATHENALSYVLGYTCGNDVTARDWQKSFGGSQWCRSK
                     SFDTFAPLGPCLVTREEIPDPQNLEVRAIVNGETRQQANTSDHIFSVTDLIVFLSGST
                     TLPAGTALFTGTPSGAGFACTPPRYLAPGDTVTIDIRGIGELTNPVVREA"
     gene            complement(1128..1949)
                     /locus_tag="PK576_08535"
     CDS             complement(1128..1949)
                     /locus_tag="PK576_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOE61677.1"
                     /translation="MAFRLSTLNLTGLFGVCAFTCLHAQLTAMPIRQPGATLDTSIRN
                     EADRAADQAAAWLISRQRPDGSWGISNRTVRSTALALLALQTSPRLTVSRSDACVRAA
                     LYLDRTSRLPLPDLETHAWRLLALAAALPHDDPERTSCLQRLAHQTEPILADAAESPR
                     RFWSEAMSAAGLTNSTPAAYPDDSARLACLANRWPPEDTPDHAVLWRCARLVNRAGKG
                     QLVRGESVLDWRKDLAVRLIRTQRRAPEAGGYWAASSTHDACEETAFGMLILLEL"
     gene            2021..2554
                     /locus_tag="PK576_08540"
     CDS             2021..2554
                     /locus_tag="PK576_08540"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOE61678.1"
                     /translation="MGWFGMAAVLTVCTFVPGLEARASIPLAFFHTGIRQCMGLPVAL
                     TICLLSNILVGVLTFWLMGPVVQMFRRWAWFERVVWPRFERTRQKLHPYVERYGEWGL
                     AIFIGVPLPGTGAYTGAFGAFLLGMDRRRFLVANALGVLIACLAVSVLCLLIDHGFVA
                     EQSLLRKLFLKSVNLNV"
     gene            2547..3575
                     /locus_tag="PK576_08545"
     CDS             2547..3575
                     /locus_tag="PK576_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HOE61679.1"
                     /translation="MCKDDGIGQAVHADAAKGRGVCLFSGGLDSQLAVCVLREQGVHV
                     EGVVFESPFFRIEGAQRSAAKLNLTLHEIPFTADILELVQHPRHGFGGALNPCIDCHA
                     RMIQRAGEWMTRNGFDFVATGEVLNQRPLSQTRHSLAVVERDAALDGRLLRPLSALLL
                     EPTLPETLGLVDRTRLLGLNGRSRKPQMELAARYGLKEYPAPAGGCLLTEKGFCRKLT
                     DLQKREGLDDERLVRLLTIGRHFRLPGSAKKCVMGRDARENALLQKIVQPDDLWLHTV
                     DIPGPTVLIPLGADDTNDRMTAAALCAAYGDHQGRSRVTVRIHTANGIEDHRVVPMER
                     ERGAAWLC"
     gene            complement(3579..4076)
                     /locus_tag="PK576_08550"
     CDS             complement(3579..4076)
                     /locus_tag="PK576_08550"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE61680.1"
                     /translation="MACLIVMDGPLKGQRVTLDQPVTRVGRREGNDWVLPDGSVSGTH
                     CEIEKREDGFLVRDLGSTNGTKVNNETVKEAPLFRNDIILMGEIPVMIEGEDVPQGNK
                     AEPITIPRTTIVIQQNRPIEPPPKEFARRSNSNAIWIALIVLLLLVIGGMLYLFFTGS
                     SVSTQ"
     gene            complement(4239..5732)
                     /locus_tag="PK576_08555"
     CDS             complement(4239..5732)
                     /locus_tag="PK576_08555"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOE61681.1"
                     /translation="MKFEEPAEPLHTTQKDPFHLADSYARILAAPLSAVLEPGTPYRS
                     GFHWTERHLQCLWYDERYRPPSFPLPGGETATVLEPGEWNLEAGPDFLNATLLIHPGA
                     RRIRGDVEVHVRPSDWDAHRHSADPAYANVIAHVTWFEGPASGSLPAGAIPLSLVAAV
                     TARPDLSLDDIDIKAYPHAALPETPRPCEILMRNQPERVRALLVSAGQYRLRVKAERL
                     RARLEQSGDRTQVVYEEIMAALGYKHNQAPFRAVARLLPVAALSAPRESAFARLLGTA
                     RLLPQPDVAPDEEGRRVIRRLWDLWWRQEGPVLPDSISWRHHNVRPQNAPVRRLAAAA
                     GLFSGITTVLHDLDRLPREEGARWFAQATGQMAARAQWTFWDQRLTFASVPTPEYPVA
                     LLGASRASALLANIVLPLYAAEGTLPPDLLEHMPSEDLSSSMRLAALYLLGRDHNPAL
                     YAGNGLLQQGLLQIHHDFCLMARPGCEACALCAVLRNLSSESPDPLR"
     gene            complement(5808..7181)
                     /locus_tag="PK576_08560"
     CDS             complement(5808..7181)
                     /locus_tag="PK576_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE61682.1"
                     /translation="MTNALFYRPLLEDLRCWRDDPNRKPLIVRGARQVGKSCLVRLFA
                     GEYPRYAELNLEKPSHAALFRRGLTLSELIQAIMLECRVPAGSSPLLLFLDEIQEVPE
                     AVAMLRFFQEERPDLHVIAAGSLLETALEAAAISFPVGRVQYLYVRPLAFAEFLAVMG
                     EDAAREALFTVPVPAFAHAHLLNLFHSYALVGGMPEAVLCYRDSGRDLLAVNRVYADL
                     LSAFRDDIPKYGRTATQRRLLLHALESAPLVAGSRVTFQGFGGSDYRSREMGDALRTL
                     ERARLLELIYPTVQGVPPLMPDRRKAPRLQFLDVGLVCHMAGLQRHLIGIRDLSDVYR
                     GRLIEQVVGQELKAIDARADLPLIFWVRDKAQSQAEVDFLLQTTAGVVPVEAKSGTAG
                     KLRSLHEYLSRYPAPLAVRVYGGMYEEHPVQVGEKTCRLVNVPYYATGLLHRYIAQAE
                     STPEHGA"
BASE COUNT         1401 a         2082 c         2441 g         1379 t
ORIGIN      
        1 gcgcttgcga cgaagggctg ggacgaagag ttcaaaaaga gcgtcgggcg caccgtaaaa
       61 cccagtgttt acggggcttt gctggaattc tacccgttca attgctcctt ttgggttcag
      121 tagtgaggcg gcgggattct gtcgccgcca ccgctttgtt ggtctgagtg aagacagtcc
      181 ctgccggtat ggcgggacgt ccacagttac gcaccatagg cgcggagccg gaccgccgaa
      241 accgtgccgc cggtaagtaa gcgtctgctt gcccggacga gcaggttatc ggctcacgct
      301 tcgcgcacca cgggattggt gagttcgccg atgccgcgga tatcgatggt cacggtatcg
      361 ccgggcgcga gatagcgggg cggcgtgcac gcgaatcccg ctccggacgg cgtgccggtg
      421 aagagtgccg tgcctgcggg cagggttgtg ctgccgctta gaaacacgat cagatccgtc
      481 acgctgaaaa tgtggtctga ggtgttggcc tgctgacgtg tttcgccgtt gacgatcgcc
      541 cgcacctcca gattttgcgg gtcggggatc tcctcgcgcg taacgagaca ggggcccagc
      601 ggtgcgaagg tgtcaaagga tttgctgcgg caccattggc tgccgccgaa acttttctgc
      661 cagtcccgtg cggtcacgtc attaccgcac gtatacccca atacatacga cagcgcgttt
      721 tcatgcgttg cgttggtaca ggttcgtccg atgatgacca ccagttcggc ttcgtaatcc
      781 acctgagtgc tgggaagccg cgtgggaatc cggatcggtg cgccgggatc cagcacggct
      841 tggggacttt tcatgaacag caccggaaaa tcgggaatcg gctgtcccgt ctctgcggca
      901 tgcgcccgga agttcagccc gatcgccaaa atgagcggcg gacggaccgg cgcgagcagg
      961 gaggtgattt cgatgcggcg tccggtaggc tcccatgttg cgaagaggtc gccttcgatc
     1021 ggttcggctg aaccgtcttc gtgaaggatg ccggtgaggg gggtgggaga ggagggagtg
     1081 agaaaacgga tgagtttcat attccgggtt ctcggagatg aagagggtta gagttccagc
     1141 agaatcagca ttccgaaagc ggtctcctca caagcgtcgt gcgtggagga ggcggcccaa
     1201 tatccgcccg cttccggcgc gcggcgttgg gtgcggatca ggcgcacggc gagatctttg
     1261 cgccagtcca ggaccgactc gccgcgtacc agttggcctt tccccgcacg gttgaccagg
     1321 cgcgcgcacc gccagagaac ggcgtgatcg ggcgtgtctt ccgggggcca gcggttcgcc
     1381 agacacgcga gccgcgcgga atcgtccggg tatgcggcgg gtgtgctgtt ggtcagaccg
     1441 gctgcggaca tggcctcgct ccagaagcgg cgcggagact ccgccgcatc agccaggatg
     1501 ggctccgtct ggtgcgcgag gcgttggaga cacgacgtgc gctccgggtc gtcgtgcggc
     1561 agggcggccg cgagggccag aagacgccag gcgtgggttt cgaggtccgg caggggcagg
     1621 cgtgacgtgc gatccagata aagcgcggcg cgcacacagg catcagaacg ggagacggtc
     1681 agtcgcgggg aagtctggag cgcgagcagc gcgagtgccg tgctccgcac cgtgcggttg
     1741 gagatgcccc aggacccgtc gggccgttgc cgcgagatca gccatgctgc agcctggtcc
     1801 gcggcccggt cggcctcatt ccggatcgag gtgtcgagtg tcgcacccgg ctgacggatc
     1861 ggcatggcag tcagttgcgc gtgcaggcag gtgaacgcgc acacgccgaa cagtccggtc
     1921 aggttcaggg tggacaggcg aaaggccatc gtttatgctc cttccttttg aaagaggata
     1981 gtttagccca atgccgggcg gaaggaaagg gtgttatagg atgggctggt tcggaatggc
     2041 ggcggttttg acggtgtgca cgtttgttcc gggattggaa gcgcgcgcgt cgattccgct
     2101 ggcgtttttt catacgggga tacggcagtg catggggttg ccggttgctc tgaccatttg
     2161 tctgctctcg aacattctgg tgggggtgtt gaccttctgg ctgatgggtc ccgtggtgca
     2221 gatgttccgg cgctgggcgt ggtttgaacg tgtggtctgg ccccgttttg agcgcacccg
     2281 ccaaaagctg catccgtatg tcgaacgtta cggcgagtgg gggttagcca tttttatcgg
     2341 agtcccgctt cccggaaccg gcgcatacac cggcgcgttt ggcgcgtttc tattgggtat
     2401 ggaccggcgc cgttttctgg tggcaaacgc tctgggtgtg ctgatcgcct gtctggccgt
     2461 aagcgtcctg tgcctgttga tcgaccacgg gttcgtggcg gaacagagtc tgctccggaa
     2521 actcttcctg aaaagcgtga acctcaatgt gtaaggatga cggaatcggg caggccgtgc
     2581 atgcggatgc cgcgaaaggc cgcggagtct gtctgttttc gggcggactc gacagtcaac
     2641 ttgccgtgtg cgtgttgcgc gagcaggggg tgcacgtgga gggggtggtt ttcgaaagcc
     2701 cctttttccg gatcgagggc gcgcagcgga gtgccgcgaa actgaacctg acactgcatg
     2761 agattccctt caccgccgat attttggaac tggttcagca tccccgtcac ggattcggcg
     2821 gtgcgctcaa cccctgcatc gactgccatg cgcggatgat ccagcgcgcc ggggagtgga
     2881 tgacgcggaa cgggtttgat ttcgtggcta cgggggaggt gctgaaccag cgtcccctgt
     2941 cccagacccg gcactcgctc gccgtggtcg aacgggatgc cgctttggac gggcggttgt
     3001 tgaggccgtt gagtgcgctg ctgctggaac cgacgctgcc ggaaacattg gggcttgtgg
     3061 accggacccg cctgctgggt ttgaacggcc ggtcgcgcaa accgcagatg gaactggccg
     3121 cgcgttatgg actcaaggag tatcccgcac ctgcgggcgg gtgtctgctc acggagaagg
     3181 ggttctgccg taaactgacg gatctccaaa aacgggaggg gctggacgac gagcgtttgg
     3241 tacgcttact gacgatcggg cgccatttcc ggctgccggg aagcgcgaaa aaatgtgtga
     3301 tgggacgcga tgcccgggaa aacgccctgt tgcagaagat cgttcagcct gacgacctct
     3361 ggctacacac ggtggatatt cccggtccga cggtgttgat cccgctgggc gcggacgaca
     3421 cgaacgaccg gatgacagcg gcggccttgt gtgcggcgta tggcgaccat cagggacgct
     3481 cccgcgtcac agtccgcatt catacggcaa acggcatcga ggatcatcgt gtggtgccga
     3541 tggaacggga acggggtgcg gcgtggctat gctgaggatc attgcgtcga caccgacgag
     3601 ccggtgaaga aaagatacag catcccgccg atgaccagca acagcaggac gatcagggcg
     3661 atccagatgg cgttggagtt tgatctccgg gcgaactcct tgggcggagg ctcgatgggg
     3721 cggttttgct gaatgacgat cgtggtgcgg ggaatcgtaa tgggttccgc tttgttaccc
     3781 tgcggcacat cctcgccttc aatcatcaca gggatctcac ccatcagaat gatgtcattg
     3841 cggaacaacg gcgcttcttt gaccgtttcg ttgttgactt tggtgccgtt ggtggaaccc
     3901 aaatctcgaa cgagaaaacc gtcttcccgt ttttcgatct cacagtgcgt tccggatacc
     3961 gagccgtctg gcaacaccca gtcgttccct tccctgcgtc cgactcgagt caccggctgg
     4021 tccaacgtta cgcgttgccc tttcaacggg ccgtccatca ctattaggca agccatatca
     4081 cgcaccttta atggaaaatt gatctatagc ttaataatcc cgccgtcgga agtcaatgcc
     4141 gcgtatcaaa atgaaacaag caaattcatt acgcgaggaa aacagaactc cgcctgttgg
     4201 ggaggcggca atggctatgg gacgcaaacc tccttgtttc atcgaagcgg atcgggactt
     4261 tccgaggaaa gattccgcaa tacagcgcac agggcacagg cttcgcatcc gggacgggcc
     4321 atcaggcaga aatcgtggtg gatctgcaat aaaccctgct gcagcagacc gttgccggca
     4381 tacagggcgg gattatggtc gcgtccgagc aggtaaagtg ccgcgagccg catggaagac
     4441 gagaggtcct cggagggcat gtgctcgagg agatccggcg gcagcgtacc ctccgccgcg
     4501 tacaatggca gcacgatgtt ggcgagaagg gcggatgcgc gggaggcgcc cagcaacgcg
     4561 accggatact cgggcgtggg caccgaagcg aaggtgagac gttgatccca gaaggtccat
     4621 tgggcgcgtg cggccatctg tccggtcgct tgggcaaacc agcgcgctcc ctcctcgcgc
     4681 ggcaggcggt cgagatcgtg cagcacggtg gtgatgccgc taaagagtcc cgctgctgcc
     4741 gcgaggcgac ggaccggcgc gttttgcggc cgcacattgt ggtgacgcca agagatcgaa
     4801 tccggcagca cgggcccctc ctgccgccac cacaggtccc agagacggcg gatgacgcgg
     4861 cggccctcct cgtccggagc gacatccggt tgggggagaa ggcgcgcggt gccaagcaaa
     4921 cgcgcgaaag ccgattcgcg cggagcactg agcgcggcca cgggcaggag gcgggcgacc
     4981 gcacggaacg gcgcttggtt gtgtttgtac ccgagggccg ccatgatttc ctcgtatacc
     5041 acctgtgtgc ggtcaccact ctgttccaag cgggcgcgta accgttccgc tttgacgcgc
     5101 aggcggtact gcccggctga gacaagcagg gctctgaccc gttccggttg gttccgcatc
     5161 aaaatttcac aggggcgcgg cgtttccggc aacgcggcgt gcgggtaagc tttgatgtcg
     5221 atatcatcca gcgagaggtc ggggcgcgcc gtcacggctg cgacgaggga gagggggatg
     5281 gcccctgctg gcagggagcc ggatgccggt ccctcgaacc aggtgacatg cgcgatgacg
     5341 ttcgcgtacg cggggtcagc actgtggcgg tgcgcgtccc aatcggaggg acggacatgc
     5401 acttcgacat cgccgcggat gcggcgcgct cccgggtgga tcaggagcgt ggcgttcaga
     5461 aaatcggggc cggcctccag attccattcg ccgggttcca gcacggttgc ggtttcgccg
     5521 ccgggcaacg ggaaactggg tggacggtag cgttcgtcgt accagaggca ctgcaggtgg
     5581 cgctcggtcc agtggaagcc ggaacggtag ggggtgccgg gctcgagaac ggcggacagc
     5641 ggagcagcca gtatcctggc gtaggaatca gcgagatgga aaggatcttt ctgcgtggta
     5701 tgaaggggct ccgccggttc ctcaaatttc atacacaaaa gataacagta tcgtttcttt
     5761 tcgtaaacaa aaattggtaa tcggttcgtg acaggggtgt gcggaggtca tgcgccatgt
     5821 tctggagtgg attcggcttg ggcgatatag cggtgcagca gccctgttgc ataatacggc
     5881 acattgacca ggcggcatgt cttctcgcca acctgaaccg ggtgttcctc gtacatcccg
     5941 ccatagacac gcaccgccag cggcgcggga tagcgcgaaa gatactcatg cagcgaccgg
     6001 agcttgccgg ccgtgccgga cttggcctcg accggcacca ctcctgcggt tgtttgcaac
     6061 aggaaatcca cctcggcctg ggattgcgcc ttatcacgca cccagaagat cagcggcaga
     6121 tcggcacggg catcgatggc cttgagttcc tggccgacga cctgctcgat caggcgtccc
     6181 ctgtatacat ccgacaggtc gcggatgccg atcaggtgcc gctgcaatcc agccatgtgg
     6241 caaacaagac caacgtcgag gaactgcaac cggggtgcct tccggcggtc gggcatcagc
     6301 ggcggcaccc cctggactgt cggatagata agttccagca ggcgggcacg ctcaagggta
     6361 cgcaaggcat ctcccatttc gcgggagcgg tagtccgagc cgccgaaccc ctgaaacgtc
     6421 acccgtgatc ccgccacaag gggcgcgctc tccagggcgt gcagcagcaa tcgccgttgc
     6481 gtcgctgtgc gtccatactt tggaatatcg tcgcggaagg ccgagaggag atcggcatag
     6541 acccggttaa ccgccagcag gtcgcgcccg ctatcacggt agcacaggac ggcctcgggc
     6601 atgccgccga cgagcgcata ggagtggaac aggttcagca ggtgggcgtg ggcaaacgcg
     6661 gggaccggca cggtgaacaa cgcctccctg gcggcatcct ctcccatgac cgccagaaac
     6721 tcggcaaagg ccagaggcct gacgtacaaa tactgcaccc gtcccaccgg aaaggagatc
     6781 gccgcggcct cgagcgccgt ctcaagcagc gacccggcgg caatgacatg cagatccgga
     6841 cgttcctcct ggaaaaagcg caacatcgcc accgcctcgg gcacctcctg aatctcgtcc
     6901 aggaacaaaa gcagcggcga ggagcccgcc ggcacccggc actccagcat aatcgcctgg
     6961 atcagctcgg acagcgtcag tccgcgccgg aacagggcgg catgcgaggg cttttccaga
     7021 ttcagctccg catagcgcgg atactccccg gcaaagagac gcacgaggca gctcttgccg
     7081 acctgacgcg caccgcggac aatcagaggc ttgcggttcg gatcgtcccg ccagcaccgc
     7141 aaatcctcta ataagggtcg gtaaaaaagc gcgtttgtca caaagtaacc cctgtttgct
     7201 ccttttcacg tcacaaaata agggcattta cccaaaatgt caagaggcag gcagtaatcc
     7261 taaaaagagt tgagccttca attctctttg gttttctggt ttc
//
LOCUS       DAOSLE010000131         7259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9381_length_7259_cov_7.806774, whole genome shotgun sequence.
ACCESSION   DAOSLE010000131 DAOSLE010000000
VERSION     DAOSLE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9381_length_7259_cov_7.806774"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK576_08565"
     CDS             <1..186
                     /locus_tag="PK576_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61683.1"
                     /translation="RSSRVECRGSRVDVPDPDTDTDTDTDSDPDPDLDETPKPRNPET
                     RNQRELKVQLLFLGSAP"
     gene            complement(365..1246)
                     /locus_tag="PK576_08570"
     CDS             complement(365..1246)
                     /locus_tag="PK576_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOE61684.1"
                     /translation="MTKYMLIATVGWYALSGVHAAEIRCPLPGKWELDTSVSDEFDAD
                     SLDKTKWWDYAPHWPGRPGVYRTLARNVSQREGCLLLTTDKVPDSEIPYEARMDGMQG
                     YTCALVKSRKKVIYGYFEARIKATPAAIRNAFWLYDPLSDDLNRKYSPGDVSEEIDIC
                     EILGKHQPGDKNQSYVTSHYTHHYLTPYYEGICNNLNVPLGVMRELAFCPSADYHVYG
                     LLWTKDELVWYVDNVESARMPTGSFAQGGFRRPLHVVLDTEVNDWSGAKVSELDARTL
                     PATAKVDWFRRWVPISK"
     gene            1552..3012
                     /locus_tag="PK576_08575"
     CDS             1552..3012
                     /locus_tag="PK576_08575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE61685.1"
                     /translation="MCSRSFVLFCFLFGGPVCAMSATEPPIRIVFPAEGARLAYTDRV
                     FVIGAVRPADVPLTLDERPITPHRTGGFLHMAPVSPGTNTLIFNAGGTVIRHTFRIDP
                     PPQSWDGCSLRAQAPLQPLGLCTGETVRLACLAPADRTVFAAVGERTLALVPQPGHAT
                     LYVAKLAFDVPVADVPVTFFATGLLDAAAAPLTARADWPALRTTGPLFAMRARSEPGG
                     GETVAFLPPDLTVRGAGFVGAHTRFWLAGERRFMESRHLTSLAPEHPLPPCDLPIPDL
                     TAGFTLNPPVGRAPDSILIVLDPGHGGASTGAVGPTGLTEKEANLIQAHRVAATLRKA
                     GFRVLLTREDDKDLDLYARVRLAYERKADAFISIHYNSCGPAGNPSESRHIATYAWNA
                     IGLRLARALHPHLAAASPAADGGVRTASFAVCRNPVVPACLLELDFITCPLGEESLQQ
                     PDQRERVAHAVLAGLRNWLTPSPRTESANNATPNKE"
     gene            3018..5417
                     /locus_tag="PK576_08580"
     CDS             3018..5417
                     /locus_tag="PK576_08580"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOE61686.1"
                     /translation="MRIPPPTRRLCLAFALLSAGSVFAADYAYQPAPITRVRLLDGFW
                     LSRAETNRLVTVWANFGKCEETGRIANFARAGQLESGPFQGTAWNDSDVFKVIEGAAH
                     TLATHPDPKLDGYLDRLIVRIAAAQEPDGYLYTARTLGATNEMTGPARWSNVQSSHEL
                     YNLGHLFEAAVAHHAATGKRMLLDVACKSADLVCRVFGPGPEQRKDVPGHEEIEIGLC
                     KLYRATGERRYLDLATFFVAMRGRKDLRGRVFGPTYQDHLPVLEQSEAVGHAVRAGYL
                     YAGIADVAALTGDTAYLAAIDRLWENTVSKKLHLSGGIGARRSGEAFGENYELPNETA
                     YLETCAAIANALWNQRMFLLHGDARYIDVLERVIYNGFLSGISLSGDTFFYPNPLESR
                     GGYARSKWFGCSCCPVNIVRFIPQIATFAYAQRDATIFVNLFLASEARLTTPAGDVTL
                     TQKTDYPWRGKVRIEVAPSRDGLAFPLRIRVPGWATGQPVPSDLYTQTRPATPEAVSL
                     SLNESPVPVRIESGYVTLDRAWKKGDAVTLTLAMPVRRIRAHDAVRDNRGRLAVERGP
                     LLYCAEGVDNNGRARSLTLAPEAVFSEASLSLSEHTVTALQTTGSRVTANLRGKRTVQ
                     PATIILIPYYAWCHRGAGEMQTWFPTGVAEADPMSDFHITASHCYVKDSPEAAIDGVL
                     PKHSHDNTINRMTWWPRKGSEEWLQVDFPEEESVCGSQVYWFDDTGKGACRLPVSWRI
                     QYADADGGWRDVATEYPVAEDRFCEARFSQPVRARRFRLSVRLQTDFSGGVLEWRLLP
                     E"
     gene            complement(5444..6979)
                     /locus_tag="PK576_08585"
     CDS             complement(5444..6979)
                     /locus_tag="PK576_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOE61687.1"
                     /translation="MSYYLGIDCSTQGMKALVIDPAASRVVAGASVRFTTDLPEFGCP
                     DGVLPHPDPLIRHADPLLWVAGLERTLKRLQDTGVEMERIAAVGGDGQQHGSVYLNRR
                     FGEVLKRLSPDTPLAGQIAPALSRPTSPIWMDSATSQACRELDARFGARLRTETGSPA
                     IERFTGPQIRHFAQRDPERYADTARIHLVSSFLCSLLIGRDAPVDAGDGAGMNLLNLR
                     TLSWDPEITEFTAPGLAEKLPTIGSGVAGGLHACFARFGLRSGIPVVVWTGDNPASLV
                     GTGAWGAGAAVVSLGTSDTFFAALDRFHTDPEGFGHVFGNPAGGFMSLTCFKNGSLAR
                     DRVREDAGVDWAFFDETAFDLTPPGNQGRLALPWFVPEITPPVLTPGLRTNFAFDAAL
                     PEVRIRAVVEAQALSLRSHSLWIGAFDTLRVTGGASKSPGIRQTLADVFQSRVESIAV
                     SDSAALGGAMLAAHAADGVPLDVLTRGLTAVTAVCEPRDDVSEVYEALLPELRALERG
                     DPI"
     gene            complement(7014..>7259)
                     /locus_tag="PK576_08590"
     CDS             complement(7014..>7259)
                     /locus_tag="PK576_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61688.1"
                     /translation="YPRVFSRLRRAAGLPITYIKVILIYISVRSAGGGVTPQTALTEH
                     RDWRRLSRIAQDLGFCTSQYYIRPLVYGCANESARVR"
BASE COUNT         1394 a         2454 c         2058 g         1353 t
ORIGIN      
        1 cgatcgtcgc gggtcgaatg tcgcgggtcg cgggtcgacg ttcccgatcc cgataccgat
       61 accgataccg ataccgatag cgatcccgac cccgatttgg atgaaacccc gaaaccccga
      121 aaccctgaaa ccagaaacca aagagagttg aaggttcaac tgctcttttt aggttcagcc
      181 ccctgatcgc aataatccgc ctccccctct ttccctccct cctaaaacca acgtggagcc
      241 tgcaacccat ttagaatcac acaaggacac aaaggggttc ccatgacaat ctattcctct
      301 tcgtgtcttt gcgcgagaaa caacttgttt tcatctgcct cattttagcg cctcttcgag
      361 ggactcattt gctgatcggc acccatctgc gaaaccagtc gacttttgcc gtcgccggca
      421 atgtcctggc atcaagttca gacacttttg cacccgacca gtcgttgact tcggtgtcga
      481 ggacgacgtg caatggacgc cgaaatccgc cttgggcaaa gctccccgtg ggcatacggg
      541 cgctttccac attgtccaca taccacacaa gttcgtcttt cgtccaaagg agcccgtaaa
      601 cgtgataatc ggcactaggg cagaacgcca gctcgcgcat gacgccgagc ggcacgttca
      661 ggttattaca gatgccctca taatacggcg tcagataatg atgagtgtaa tgtgacgtaa
      721 catacgactg gtttttgtcg cctggctggt gcttgccgag aatttcacag atgtcgatct
      781 cctccgacac atcgcctggc gagtactttc tgttcaaatc atcacttagc gggtcataaa
      841 gccagaatgc atttcgtatc gcggctggcg ttgccttgat gcgcgcttca aaatagccgt
      901 atatcacttt ctttcggctt ttcacaagtg cgcacgtata cccctgcatg ccatccatcc
      961 gggcttcata cgggatctct gaatccggca ctttatcggt cgtcaacaaa aggcatcctt
     1021 cccgctgcga aacattccga gccaacgtcc gatagacacc cggccgaccc ggccaatggg
     1081 gcgcataatc ccaccatttc gtcttgtcga gcgaatccgc gtcaaactca tcgcttacgc
     1141 ttgtatccaa ttcccatttc ccgggcaacg ggcagcgaat ctccgccgca tgaaccccgg
     1201 acaacgcgta ccaacccacc gttgcgatca acatgtattt cgtcatttgc ctgtacctcc
     1261 tgttctctca cctttgcatg cgtagacact ccttcttgag aagaagtttc tgagcccgct
     1321 cgaccttggc gcggacaatc ggcgtgggta aatatggtat cacaatcttc agaagcaaac
     1381 aggatcgatt acgaaacaaa ttcaggaaaa acgaattttt taccacatag aaaaaaggtt
     1441 ttcattttct cgacctcaac gcctgaatcg ctttttcaaa ttccgcccca catgtgtaaa
     1501 tcgaaaacac ccggttttac attccgttac ggctttgcta cgctcccggg catgtgttcc
     1561 cggagtttcg tcctcttctg cttcctgttc ggaggccccg tatgcgcgat gtccgccacg
     1621 gaacccccga tccgcatcgt cttcccggct gaaggcgcgc gactggcata cacggaccgc
     1681 gtgtttgtga tcggcgcggt gcggcctgcc gacgtgccgc tgacgctcga cgaacgcccg
     1741 atcacaccgc accgcaccgg cggattcctg cacatggctc cggtctcccc cggcaccaac
     1801 accctgatct tcaacgcggg agggaccgtg atccgtcaca ccttccgaat cgatccgccg
     1861 ccccagagtt gggatggctg ttccctacgc gcgcaggctc cactccaacc gctggggctc
     1921 tgtaccggcg aaacggtccg gctcgcctgc ctcgcgccag cggaccgcac ggtcttcgcc
     1981 gcggtgggcg aacggaccct cgcactcgta ccgcaacccg gccacgccac gctttacgtg
     2041 gccaagctcg cgtttgacgt gccggtcgcc gacgtgccgg tgaccttttt cgccaccggg
     2101 ttgttggacg ccgcagcagc cccgctcacc gcacgcgccg actggccggc cctacgcaca
     2161 accggccccc tcttcgcgat gcgcgcgcgt agcgaaccgg gcggcggcga gaccgtggcc
     2221 tttttgcctc ctgatctgac cgtgcggggc gccgggttcg ttggcgcaca cacccgtttc
     2281 tggctggccg gcgaacgccg gttcatggag tcgcgtcatc tcacttccct cgcgccggaa
     2341 catcccctgc cgccctgcga cctcccgatt cccgatctca cggccggatt caccttgaac
     2401 ccgcccgtgg gccgcgcgcc agactccatt ttgattgtgc tggaccccgg acacggcggc
     2461 gcttcgaccg gcgcggtagg ccctaccggg ctgacagaaa aagaggccaa tctgattcag
     2521 gcgcaccggg tggccgccac gctccgcaag gccggattcc gcgtgttgct gacgcgtgag
     2581 gatgacaagg atttagacct gtatgcgcgt gtgcggctgg cctatgagcg aaaggctgac
     2641 gccttcatct cgatccatta taactcctgc ggacctgcgg gcaacccctc cgaaagccga
     2701 cacatcgcca cctacgcctg gaatgcgatt ggcctccgtc tggcccgggc cctccacccg
     2761 catctggctg cggcgtcccc tgcggctgac ggtggtgtgc gcaccgccag cttcgcggtt
     2821 tgccgcaatc ccgtcgtccc cgcctgcctg ctggaactcg atttcatcac ctgccccctc
     2881 ggcgaggagt cgcttcaaca acccgatcaa cgcgaacgcg tggcccacgc ggttctggcc
     2941 ggtctgcgca actggctcac gccttcgcca cgtaccgaat cggccaataa cgcaacacca
     3001 aacaaggagt gaaaccgatg cgcattccac cccccacccg tcgcctttgt ctggctttcg
     3061 cactcctgtc cgcaggctcc gtgtttgcgg cggactacgc ctaccagccc gcgccgatca
     3121 cgcgagtgag gctgctggac ggtttctggc tgtcgcgcgc ggaaaccaac cggctggtta
     3181 cggtctgggc caatttcggc aagtgcgagg agaccggacg tatcgcgaac ttcgcgcgcg
     3241 cgggccaact cgagtccggt ccctttcaag gcacggcttg gaatgattct gatgtcttca
     3301 aggtcatcga gggtgcggcc catactctgg ccacacatcc cgacccgaag ttggacggat
     3361 atctcgaccg gctgatcgtc cgcatcgcag ccgcgcagga gcccgacgga tatctctaca
     3421 ccgcgcgtac gctcggagcc acaaatgaaa tgacaggtcc cgcgcgctgg agtaacgtgc
     3481 agtccagcca cgaactctat aacctcgggc acctcttcga agccgcggtc gcgcatcacg
     3541 ccgcaaccgg caaacggatg ttgctggacg tggcgtgcaa aagcgcggat ctcgtctgcc
     3601 gggtgttcgg tccaggaccg gagcaacgca aagatgtgcc gggccatgag gagatcgaaa
     3661 tcggtctctg caaactctat cgcgccaccg gcgaacgccg ctatctcgat ctcgccacat
     3721 ttttcgtcgc catgcgcggc cgcaaggact tgcgcggacg ggttttcgga ccgacctatc
     3781 aggaccacct gccggttctc gaacagagcg aagccgtagg gcacgccgta cgggccggtt
     3841 acctctatgc gggcattgcc gacgttgcgg cgttgaccgg cgacacggcc tatctggccg
     3901 cgatcgaccg cctgtgggag aacacggttt caaaaaagct gcatctctcc ggcggtatcg
     3961 gtgcgcgccg cagtggcgag gcgttcggtg agaactacga actgccgaac gagaccgcct
     4021 atctggagac ctgcgccgct atcgccaacg ccctctggaa ccagcgcatg tttctgctgc
     4081 acggcgacgc ccgatatatc gatgttctgg agcgcgtgat ctacaacggg ttcctctcgg
     4141 gtatcagcct ttccggcgac acgttcttct atcccaaccc gctcgagagc cgcggcggct
     4201 acgcgcgctc caaatggttc ggctgctcct gctgtcccgt gaatatcgtg cgcttcatcc
     4261 cgcagatcgc cacgttcgcc tacgcgcaac gcgacgccac catcttcgtg aatctcttcc
     4321 tcgccagcga ggcccggctg acgactccag ccggcgacgt gacgctgacg caaaagaccg
     4381 actacccgtg gcggggcaag gtacggattg aggttgcccc ctcccgtgac gggctcgcct
     4441 tcccgctccg gatccgcgtg ccgggatggg ccacggggca gcccgtaccc tccgatctct
     4501 atacacagac ccggcccgcc acgccggaag cggtttccct ctccctgaac gaatcgcccg
     4561 tgccggtgcg gatcgagagc gggtacgtca ccctcgaccg cgcatggaaa aaaggcgatg
     4621 ccgtgaccct gacgctcgcc atgcccgtac gccggatccg ggctcatgac gccgtgcgtg
     4681 acaaccgcgg tcggctcgcg gtcgaacgcg gtccgctgct gtactgcgcc gaaggtgtgg
     4741 ataacaacgg ccgcgcacga tcgctcacgc tcgcgccgga agcggtcttt tctgaagcgt
     4801 cccttagcct ctccgaacac acggtgaccg ccctgcagac caccggaagc cgcgtgaccg
     4861 cgaacctgcg gggtaaacgg accgtgcaac ccgcaacgat aatcctgatc ccctactatg
     4921 cctggtgcca ccgtggcgcg ggcgagatgc agacctggtt cccgaccggt gtggccgagg
     4981 cggatccgat gtcggacttc cacatcaccg cctcgcactg ctatgtgaag gattcgcctg
     5041 aagcggccat agacggggtg ctgcccaaac attcccatga caacacgatc aaccgcatga
     5101 cctggtggcc gcgtaaagga agcgaagagt ggctgcaggt cgattttcct gaggaggagt
     5161 ccgtctgcgg aagtcaggtc tactggtttg acgataccgg caaaggcgca tgccgcctgc
     5221 ccgtctcgtg gcgcatccaa tacgcggacg cggacggcgg ctggcgcgat gtggcgaccg
     5281 aatacccggt tgcggaagac cggttctgcg aggcgcgttt ttcgcaaccg gtccgtgccc
     5341 gccggttccg tctgagcgtg cggctccaaa ccgatttctc cggcggcgtt ctcgaatggc
     5401 ggctgcttcc cgaatagcct gccgtgccga ccccggtctc tcttcagatc gggtcgccgc
     5461 gctccagtgc gcggagttcg ggcagcaacg cctcatagac ttcggaaacg tcgtcgcggg
     5521 gttcgcacac cgccgtcacg gcggtcagac cgcgcgtcag aacatcgagc ggcacgccat
     5581 ccgcggcatg cgccgccagc atcgcgccgc ccaacgctgc ggaatcggac accgcgatgc
     5641 tctccacacg cgactggaac acgtcggcca aggtctggcg gatcccggga ctcttcgagg
     5701 caccgccggt gacgcgtaac gtgtcaaacg cgccgatcca caaggaatgg ctgcgcaggg
     5761 acaaggcttg cgcctccacc accgcacgga tacgcacctc cggcagcgcg gcgtcgaacg
     5821 caaagttcgt ccgcaggccg ggtgtcaaca ccggcggcgt gatctccggc acaaaccacg
     5881 gcaacgccag tcgcccctga ttgccgggcg gagtcagatc aaatgccgtc tcatcgaaga
     5941 atgcccaatc cacgcctgcg tcttcgcgca cccgatcgcg cgccagcgag ccattcttga
     6001 aacaggtcag gctcatgaat ccacccgccg gattgccgaa aacatgtccg aacccctccg
     6061 ggtcggtgtg gaaccggtcg agtgcagcga aaaaggtgtc gctggtgccg agactcacca
     6121 ccgccgcgcc cgcgccccac gcgccggtgc ccaccagact ggcgggatta tcgccggtcc
     6181 acacaaccac cgggatgccg gaccggaggc cgaagcgggc aaagcaggca tgcagaccgc
     6241 ccgccacgcc gctgccgatc gtcggcaact tttcggccag acccggagcg gtgaattccg
     6301 tgatctcggg atcccacgag agggtccgca gattgagcag gttcatgccc gcaccgtcgc
     6361 cggcatccac cggcgcgtct ctcccgatca gaagggaaca aagaaacgaa ctgacgagat
     6421 ggatgcgcgc agtgtcggca taccgctcgg gatcgcgctg ggcgaagtgc cggatctgcg
     6481 gaccggtgaa acgctcaatg gcgggcgacc cggtttcggt ccgcagacgc gcaccgaacc
     6541 gtgcgtccaa ctcccggcat gcctgtgagg tggcgctatc catccagatc ggactcgtgg
     6601 gccgggacaa cgcgggagcg atctgcccgg ccagcggcgt gtcgggcgag agccgtttga
     6661 gcacctcgcc gaaccggcgg ttgaggtaga ccgaaccgtg ctgctgtccg tccccgccca
     6721 ccgccgcgat acgttccatc tccacgcccg tatcctgaag ccgcttcagc gtccgttcca
     6781 accccgcgac ccacaacaac ggatcggcat gccggatcaa cggatcgggg tgcggcaata
     6841 cgccgtcagg gcatccgaac tcaggcaggt ccgtggtgaa ccggacggac gcaccggcca
     6901 ccacgcggct tgcggccgga tcgatcacca acgccttcat accctgtgta ctacagtcga
     6961 ttccaagata atacgacatg ccgaacctcc ctgtaatcaa acccgaccgg tgttcagcgt
     7021 acccgagccg actcgttcgc gcaaccgtaa acaagcggcc ggatgtagta ctgtgaggtg
     7081 cagaagccca gatcctgcgc aatgcgactc aaccgcctcc agtcgcggtg ttcggtaagt
     7141 gccgtttggg gggttacccc gccgcccgcg cttcgcacgg aaatgtaaat gaggataact
     7201 ttgatgtaag ttataggcaa gcccgccgcg cgccggagcc gcgagaaaac tctgggata
//
LOCUS       DAOSLE010000132         7198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9465_length_7198_cov_7.693406, whole genome shotgun sequence.
ACCESSION   DAOSLE010000132 DAOSLE010000000
VERSION     DAOSLE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9465_length_7198_cov_7.693406"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK576_08595"
     CDS             complement(<1..227)
                     /locus_tag="PK576_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOE61689.1"
                     /translation="MSMNRRGFLKNAAWAGGAVGVGAWSGCMSSSGISARKGGSMAGF
                     RCEPMATVRVGVIGVGHRGPGAVRRLSSIPGV"
     gene            complement(289..1962)
                     /locus_tag="PK576_08600"
     CDS             complement(289..1962)
                     /locus_tag="PK576_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61690.1"
                     /translation="MQQNGLAFADYAVVVSFFAVMISVGVFFGRSQKNMAQFFGGGKQ
                     VPWWLSGISFYMCSFSALAFVMYSALAYKFGWLPITISWLSVPAVFIGCRLFAARWRR
                     AAETSPLEFIERRYGNSMRQGLMWLGLPMRVLDDAFKLLAIGTVVGAGMGFPLHGAII
                     ASGLIILSYTFMGGLWATLVADFIQFFVLFTVILVLPFLCLDKAGGWSGFVENVPPGF
                     FRLTADKYNWTYMLVFFLMLFFNLSTSWSMVQRYYSTRSEKDARKVGYFVCILLSLGP
                     PLFFIPAMAARVFMPEIPADRMNEVYALVCKNVLPAGFMGMVIAAMFSATMSTLAGDY
                     NAVASVVTNDFYKRMIAPNASPKQHMLVARLATLLVGLAVIGLTFVMQNAQGANDLFD
                     TANKVFGIFLPPIAMPTMLGLVTRRITRRGGMAGLWGGVIVGLVTFIAGARWPVLRVT
                     SVIFFITCFATLAGLFLGSLLLKDDPAHMESLNSFFEKVNTPSPVLEGPPARITFWPL
                     VAGGLAVIGTVLIVSSAATRPFAEIRLSVFGGMGLLVVAGIFFRLSRVR"
     gene            complement(1965..2963)
                     /locus_tag="PK576_08605"
     CDS             complement(1965..2963)
                     /locus_tag="PK576_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61691.1"
                     /translation="MRKTVRIAAAFVLACGAVYAEVTQVGIIGLDTSHTIAFTKMLNV
                     EKGEPLFESFRVTAAYTYGSLDIPSSTNRYPAYTKQMVEMGVEIVGSIAELLKRSDVI
                     LLETNDGRRHYEQALEVFKAGKRVFIDKPIAASLTDAVRIFDAAKHYGVPVFSSSALR
                     YSTKIQKAHAGAFGRISGADAYTPCALEPTHPDLFWYGIHAAEPLFTVMGTGCESVVR
                     VSREKVDFVVGVWSDGRVGTLRGIRDKPAPYGCEIFAEKGGRLALGGYEGYKPLLIEI
                     LKFFQTGRVPVSPDETLEIFAFLEAAHESTRRGGVPVKVAEVLDQARAEAARKGMK"
     gene            complement(2974..3870)
                     /locus_tag="PK576_08610"
     CDS             complement(2974..3870)
                     /locus_tag="PK576_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOE61692.1"
                     /translation="MSPVMRHLSRRMFSAQALLAAGGWAAGVWRASADAKRPLKVYIF
                     ADMEGISGITHSGQTLADGAAYGEGCRLMEADINACVAGCFDAGATEVTVRDGHGRGN
                     NVDPARVDPRARLVQGPTPGVRFGGFEGSAALILLGYHAKALTPEALLAHSYSSLTIQ
                     GMWLNGREAGEIGVDTAIAAEHGVPVVMVSGCDKAVAEAREWIPGVVTVQTKRSTGVQ
                     SAVCLPLESARRMIREKAAEAVRRRQAIAPVAVAYPVTLRMDFLPQGSLRTYDPAFTP
                     TSSPRRVERTGDSVEKLLLNKN"
     gene            complement(3888..5216)
                     /locus_tag="PK576_08615"
     CDS             complement(3888..5216)
                     /locus_tag="PK576_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61693.1"
                     /translation="MNRRDFLKTTVYAGAVAMGARTASAQASPAERVRVAVMGTGGRG
                     ASLMKTAAELPNVEIATVCDVDVRAMDRAAETVSKKTGKTPKKEKDLRRILEDKEIDG
                     VICATPDHWHAPAALMTMRAGKAIYVEKPCSHNPREGEMLVEASVRHNAVFQMGNQRR
                     ASMTYRQVIREIQGGLIGEPRFARCWYTTQRGPIGKGKAVPVPEWLDWDLWQGPAPRR
                     PFRDNIVPYTWHWFFHWGTGESGNNATHFVDVARWTLGGRFPTRVTSAGGRLFYEGDD
                     WEWYDTQTATFEYPNRTFITWEGLSAVKARPYEGIGTGCMVYGLKGAVLFAPNNTCRH
                     FDPAGKVVKNWDAQSLAGDAMDRANPIGGLDAAHLGNWADCIRARDRAKAYSPADEAH
                     ASTLMTHLANIAQRTGETIRTDPATGRLAQGSPGAELWAREYEKGWEASV"
     gene            complement(5488..7071)
                     /locus_tag="PK576_08620"
     CDS             complement(5488..7071)
                     /locus_tag="PK576_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61694.1"
                     /translation="MKAFLNGSVVLLCFAFALPRRGDAEPVLLFSDTFDYVTSGSVSN
                     PSSSTWKSSELYSMYTNIYSAGGSVKLGKSNEGGILTTTNLAVTAGLLSVQVDALGWD
                     SDEKGFMVSVGNMTTNLTCTNDKSASTLFETVSTTFEVETGVIFVTFQTENGKRIFLD
                     NIQITHTPEGGPVPLAFFVYPHSATTVPALTEIAFTITALADGVATNVSYAGGLPDGA
                     LYDFIGQRFAWTPELNHTGEFALVFAATDTAGTVHQKTVPVTVTPLPLHAPTGVTVTE
                     LTCKTVSLAWDAVPAASHGYRVSAWYGSTAPDMLSVDGETFFEIMDTGLVAAPTGWLF
                     QGVTEKYSTADFVELKFNDTGDSVITKRYPAPVSSLSFRLKGYATSSLSNSLFTVYGS
                     CGGDDWTLLRQYSSLEDSDGDDENDLFTSAEGDLDKTVTLHAAEGYRQFRFVYGQKAS
                     GNIGLANVTAAYAGSGARFVDGWQATPVSATNLRITGLPPAHEILIRIEALDATESRA
                     TQLRLSSPPFLPKTLLMIR"
BASE COUNT         1462 a         2434 c         2025 g         1277 t
ORIGIN      
        1 actcccggaa tgctcgacaa ccgcctcacc gcgccgggtc cgcggtgtcc gacaccgatc
       61 acacccacac ggaccgtggc catcggctcg caccggaaac cggccatcga cccgcctttg
      121 cgcgcgctga tgccagaaga tgacatacaa cccgaccatg cgccgacgcc gaccgcccct
      181 ccggcccacg ccgcattctt caaaaaccct ctgcgattca tgctcatccc gcccctcctt
      241 tgttggtttg tgagctatgg ttccgacacc gcaccgcgcg gccgtcgttt accgcacccg
      301 cgaaagtcgg aaaaagatcc cggccacaac cagcaggccc atcccaccga ataccgagag
      361 ccggatctcg gcgaacgggc gcgtggcggc cgacgagaca atcagcaccg tgccgataac
      421 ggccagtccg cccgccacca gcggccagaa cgtgatacgg gcaggcggtc cctccagcac
      481 cgggctcggc gtgttgacct tctcgaaaaa gctgttcagc gattccatgt gcgccgggtc
      541 atcctttaac agcaaggaac ccaaaaacag tccggcaagc gtggcgaagc aggtgatgaa
      601 aaagatcacg ctggtaacac gaagaaccgg ccaacgcgcg ccagcgataa acgtcaccag
      661 accgacgatg acacctcccc ataggccggc catacccccg cggcgcgtga tgcggcgcgt
      721 caccagtccc aacatggtcg gcatggcgat cggcggcagg aatatgccga aaaccttgtt
      781 ggctgtatcg aacaggtcgt ttgcgccttg cgcgttctgc atcacgaaag tgagcccgat
      841 caccgccagc ccgaccagca gcgtcgccag acgggcgacc agcatgtgct gcttgggcga
      901 ggcgttcggg gcgatcatcc gcttgtagaa atcattggtc accacagaag ccacggcgtt
      961 gtagtcgccg gcgagcgttg acatggttgc cgagaacatg gccgcaatga ccatgcccat
     1021 gaaccctgct ggcaaaacat tcttgcaaac caacgcgtac acctcgttca tgcgatcggc
     1081 gggtatttca ggcatgaaga cacgggccgc catggccggg atgaagaaaa gcggcggacc
     1141 gagggagagc agtatgcaaa cgaaatagcc gaccttgcgg gcgtctttct cgctgcgcgt
     1201 ggaataatat ctttgcacca tggaccagga tgtcgaaaga ttgaagaaga gcatcaggaa
     1261 aaagaccagc atgtaagtcc agttgtactt gtctgccgtc aaacggaaga accccggtgg
     1321 cacattctcg acaaatcccg accaaccgcc tgctttgtcc agacagagga acggcaggac
     1381 caggatcacg gtgaacagca cgaagaactg gatgaagtcc gcgaccagag tcgcccacaa
     1441 accgcccatg aaggtgtaac taaggatgat caaccccgag gcgatgattg cgccgtgcag
     1501 ggggaagccc atgcccgcac cgactaccgt gccgatggcc agcagcttga acgcgtcatc
     1561 gagcacgcgc atcggaagcc ccagccacat cagcccctga cgcatgctgt tgccgtaacg
     1621 ccgctcgatg aactccagcg gactggtttc agccgcgcga cgccagcgcg ccgcgaaaag
     1681 ccggcacccg ataaatacag ccggcacgct caaccagctt atcgtgatcg gcagccagcc
     1741 gaacttgtag gccagcgcgg agtacatcac aaaggccagc gcgctgaagc tgcacatgta
     1801 gaaagaaatg ccgctcagcc accagggcac ctgcttgccg ccgccgaaaa actgcgccat
     1861 gttcttctga ctgcggccga agaacacccc gactgaaatc atcaccgcaa agaagctgac
     1921 gaccaccgcg taatccgcaa aggcgagtcc gttttgctgc atggtcactt catccctttc
     1981 cgcgccgctt cggcgcgcgc ctgatccaga acctctgcca ccttcaccgg gacgccaccc
     2041 cgccgcgtac tttcgtgcgc ggcctccaga aaggcgaaga tctccagcgt ctcgtccgga
     2101 gagaccggca cccggccggt ctggaaaaat ttgaggattt cgatcagcag gggcttgtac
     2161 ccctcatagc cgcccaacgc gagacgcccg cccttctcgg cgaagatctc acacccgtaa
     2221 ggcgcgggct tgtcgcggat cccccgcagc gtgcccacac gtccgtcgct ccacaccccc
     2281 accacgaaat ccaccttctc ccggctcacg cgcaccacac tctcgcagcc cgtgcccatc
     2341 accgtgaaga gcggctccgc agcatggatg ccataccaga acagatccgg atgggtcggc
     2401 tccaacgcgc agggcgtgta ggcgtccgcg ccggaaatcc ggccaaacgc cccggcgtgc
     2461 gccttctgaa tctttgtcga gtaccgcagg gccgacgagc tgaagaccgg cacgccgtaa
     2521 tgcttggctg cgtcgaaaat ccgcaccgca tccgtgagcg atgcggcgat cggtttatcg
     2581 atgaagaccc gcttacccgc cttgaacacc tccagcgcct gctcgtagtg acgccggccg
     2641 tcattggtct ccagcagaat cacatcgcta cgcttcagca gttcggcgat ggagccgacg
     2701 atttcgaccc ccatctccac catctgtttg gtgtaggcag gataccggtt ggtgctggac
     2761 gggatgtcca gactgccata ggtatatgcc gcggtcacgc ggaaggattc aaacaacggt
     2821 tcgcctttct cgacgttcag cattttggtg aacgcgatcg tgtgtgacgt gtccaacccg
     2881 atgatcccca cctgtgtaac ctctgcgtac accgcgccgc atgccagaac aaacgccgcc
     2941 gcaatcctta ccgtcttcct catcctctcc ccctcagttc ttgttgagca acagcttctc
     3001 cacagagtcg cccgtccgtt ccacccgccg cggcgacgac gtcggcgtga acgccgggtc
     3061 gtatgtgcgc agcgatcctt gtggcagaaa gtccatgcgt agtgtgacgg gatacgccac
     3121 cgcaaccggc gcgatcgcct gccgcctgcg gaccgcctcg gcggccttct cccgaatcat
     3181 ccgccgtgcc gattccagcg gcaggcatac ggcactctgc acacccgtac tgcgcttcgt
     3241 ctgcactgtc accacgccgg gaatccactc ccgcgcctcg gccacagcct tgtcgcaccc
     3301 cgaaaccatc accaccggca caccatgctc cgccgcgatc gccgtatcca cgccgatctc
     3361 gcccgcctcg cgcccgttca gccacatgcc ctgaatcgtg agcgatgaat acgagtgcgc
     3421 cagcagcgcc tcgggcgtca acgccttcgc gtgatagccc agcaaaatca gcgccgcact
     3481 gccttcgaag cccccgaagc gcacccccgg cgtggggccc tgcaccagcc gcgcgcgcgg
     3541 gtcgacccgc gcgggatcga cgttgttgcc gcggccgtgc ccgtcgcgca ccgtgacttc
     3601 cgtcgcccct gcgtcaaaac agcccgccac gcaggcgttg atgtccgcct ccatcaggcg
     3661 gcaaccctcg ccgtacgcgg caccgtccgc aagcgtctgc ccgctgtggg tgatgccgct
     3721 gataccttcc atgtccgcaa agatgtagac cttgagtggc cgcttggcat cggcggatgc
     3781 ccgccagaca cctgcagccc accctcctgc ggccagcagg gcctgtgctg aaaacatgcg
     3841 tcgcgacagg tgccgcatca ccggactcat cctttcccct ccgcgcatca gacgctggct
     3901 tcccacccct tctcgtattc ccgcgcccac agttccgcgc cgggcgagcc ttgggccagc
     3961 cgacccgtcg cgggatcggt ccggatcgtc tccccggtac gctgggcgat gttcgccaga
     4021 tgggtcatca gcgtactggc gtgtgcctca tcggcaggcg aataggcctt cgcgcggtcc
     4081 cgggcacgaa tgcagtcggc ccagtttccg agatgggccg catcgagccc gccgatcgga
     4141 ttcgcacggt ccatcgcgtc tcccgcaaga gattgcgcgt cccagttttt caccaccttg
     4201 cccgccggat caaaatgccg gcaggtgttg ttgggcgcga acagcaccgc acccttgaga
     4261 ccgtaaacca tgcagccggt accgatcccc tcgtaaggac gggctttgac cgccgagagt
     4321 ccctcccacg tgatgaacgt gcggttggga tactcgaacg tcgcggtctg cgtgtcatac
     4381 cactcccaat cgtccccctc gtaaaagaga cgcccgcccg cgctggtgac gcgcgtcggg
     4441 aaccgtccgc ccaacgtcca gcgcgcgaca tccacgaaat gggtcgcatt gttgccgctc
     4501 tcgccagtgc cccagtggaa aaaccagtgc cacgtgtacg gcacgatgtt atcgcggaac
     4561 gggcggcgcg gcgcgggccc ctgccataaa tcccaatcca gccactccgg caccggcacc
     4621 gccttgcctt tgccgatcgg gccgcgttgc gtggtgtacc agcagcgcgc gaaacgcggc
     4681 tcgccgatga gtccgccttg gatttcgcgg atgacttgcc gataggtcat tgaggcgcgg
     4741 cgctggttgc ccatctgaaa aaccgcgttg tgccgaaccg aggcctccac cagcatctca
     4801 ccctcgcgcg gattgtggga gcagggcttc tccacataga ttgccttgcc ggcccgcatg
     4861 gtcatcagcg cggccggcgc gtgccagtga tccggcgtgg cgcagatcac accgtcaatc
     4921 tccttgtcct ccagaatccg acgcaggtct ttctctttct tcggagtctt gccggttttc
     4981 ttgctcaccg tctcggcagc ccggtccatc gcgcgcacgt ccacatcaca gaccgtcgcg
     5041 atctcgacat tcggcagttc cgcggccgtc ttcatcaggg acgcgccgcg gccgcccgtc
     5101 cccatcaccg ccacccgcac gcgctcggca ggcgatgcct gcgcggaggc cgtgcgcgcg
     5161 cccatagcaa ctgctccggc gtaaaccgtt gttttcagaa aatccctgcg gttcatgctt
     5221 cctccccttt ttcagattcg cacccataaa ctccgacata acaggatagc ggaatcgacc
     5281 cgtttccatc aattgcatta tgacgtatgc ggagtgcaaa aaaaccggcg tcttggttcc
     5341 tccctcgatt gactcgcaag gccgttgatt ccaaacccga tgcctcaagt cggcaggaac
     5401 gagcgccccc gcgagccgca gacagcccct gccagtacgg cgggacgtcc accgctacgc
     5461 gccatcggca cggagccgga cactccgtca ccgaatcatg aggagggtct tgggcaggaa
     5521 cgggggagaa gacaggcgga gttgggtcgc gcggctctcc gtggcatcca gcgcctcgat
     5581 acggatgagg atttcgtgcg cgggcggcaa accggtgatc cgcaaattcg tggcggagac
     5641 cggagttgcc tgccagccgt ccacgaaacg ggctccgctc ccggcgtacg ccgcagtcac
     5701 gttggccaac ccgatgttgc cgctcgcctt ctgcccatac acaaaacgga attgccggta
     5761 gccttcggcc gcatgcaggg tcaccgtttt atcgaggtcc ccctccgcgc tggtgaagag
     5821 gtcgttctcg tcatctccgt cagaatcctc cagcgaactg tactgccgga gcagtgtcca
     5881 atcgtcgccg ccacatgaac cgtacaccgt gaaaagactg ttggacagtg aactcgtggc
     5941 ataccccttg aggcggaaag agagcgagga aaccggagcg ggataacgtt tggttatcac
     6001 actgtcgccg gtatcattga atttcaactc cacgaaatcc gctgtggagt atttttccgt
     6061 cacgccctgg aacaaccacc ccgttggcgc ggcgaccagc cccgtgtcca tgatttcgaa
     6121 gaaggtctcc ccatcaacgg aaagcatgtc gggtgccgta ctcccgtacc aggcggagac
     6181 ccggtaaccg tgcgatgccg caggaaccgc atcccacgcg agggagaccg ttttacaggt
     6241 gagttccgta accgtgacgc cggtcggcgc atgcaaggga agaggggtga ccgtaaccgg
     6301 aaccgttttc tgatggaccg tccccgcagt atccgttgcc gcgaagacca gagcgaactc
     6361 gccggtgtgg ttgagttccg gcgtccaagc gaaccgctgg ccgataaagt cgtagagcgc
     6421 cccatccggc agtccgccag cgtacgacac gttggtggca acgccgtcgg ccaaggccgt
     6481 gatcgtgaac gcgatctccg tgagcgcggg cacggtcgtg gcggaatggg ggtaaacaaa
     6541 gaacgccaac gggaccggtc ctccttcggg cgtatgggtg atctgaatgt tgtcgagaaa
     6601 gatccgcttg ccgttttcgg tctggaacgt tacaaaaatg actccggtct caacttcgaa
     6661 tgttgtggac accgtctcga acagcgtgga ggcggacttg tcgtttgtgc aggtcaggtt
     6721 ggtggtcatg ttcccgaccg aaaccatgaa ccccttctca tcgctatccc agcccaaggc
     6781 gtcaacctga accgacagga gacctgccgt cacggccaga ttggtggtgg tcagaatacc
     6841 gccctcattt gatttgccga gtttgacact ccctccggca gagtagatgt ttgtgtacat
     6901 cgaatagagt tctgacgatt tccatgtgga actgctcgga tttgaaacgg aaccggacgt
     6961 gacgtaatcg aacgtgtcgc tgaacagcaa gaccggttcg gcgtcacccc gtcgcggcaa
     7021 agcgaaagca aaacaaagta aaacaaccga accattcagg aacgctttca tgttccctcc
     7081 cttcagttac tttacggtca catgccggtg atcaaggcag gctccaaata aaggatacac
     7141 ttaacgaggc tcttgcaaaa ccccccgctc cgcagcgcct gacggcctgc gcgcgggc
//
LOCUS       DAOSLE010000133         7146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9541_length_7146_cov_8.395572, whole genome shotgun sequence.
ACCESSION   DAOSLE010000133 DAOSLE010000000
VERSION     DAOSLE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7146
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9541_length_7146_cov_8.395572"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..710
                     /gene="def"
                     /locus_tag="PK576_08625"
     CDS             168..710
                     /gene="def"
                     /locus_tag="PK576_08625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE61695.1"
                     /translation="MILPIVTFGHDVLRQKATPVPEVNEDVRQLVRDMLESMYAAKGL
                     GLAAEQIGRTESVCVIDVPKEAEKEECREENAAIPMPLILINPEITATQGRQRNEEGC
                     LSFPEIGAPITRADRVTVSFTDLDGNRQTLSARGLLSRAIQHETDHLNGVLLVDRMSP
                     LQRMSVSGQLKRLQKAAQTE"
     gene            770..3307
                     /locus_tag="PK576_08630"
     CDS             770..3307
                     /locus_tag="PK576_08630"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61696.1"
                     /translation="MKLMNLMPLCGAFALCALTCSASDWLSPAAPGRAYPEDEADRQW
                     AVLSSDLTRKARFDQFAAETVCAEALITATDRDPLDILLRRTAALLADLRRQTGVRDL
                     TPESRELAALREQAETTNPEQVAARRAFFDRALRLRRRIAFANPLLDFREMLFIKREL
                     RGVMEHCCDQFYGQQQRSGGGLFVLADPFGDRPALRDMLANARVTNGPLKGQPLAFGT
                     RATQGKDGGSIVSPALDYDGKRIAFAYVEGVGSRKHIRHLDHARDGHWERGFCYHIFS
                     AGTDGTRLTQLTDGTFNDFQPCFTPAGRIAFISERRGGYLRCGRHCPAFTLFDMAEDG
                     SDIRCLSFHETNEWSPAFTHDGMLLWTRWDYVDRHGCVAHKPWLTTPDGRNPRPVHGN
                     YSLRPLRPDMELDTRPIPESHLLLATAAPHHGQAFGSLVLIDPRAEDDDAMGPVRRLT
                     PDAGFPESQKGSQTYGTPWPLSKHYALCAYTPVEALGTGQRHLFGLYLLDCFGNKELI
                     YRDPGIACLNPIPLRASPRPPVLPEQRQRIASGERGEATVMIADVYKSQMPWPEGTVI
                     TALRVWQIFPLSVASAEVTHNIGIQIIEGHDSINLARVVLGTTPVEKDGSAHFTVPAG
                     VELFFQALDADGCAIQSMRSATAFMPGERASCQGCHEPKGGAPEPAPKTVTLAMRRAP
                     SALVPGPEGSNPFSFPRLVQPVLDRYCVGCHAETLRTAKPGAKMPPRLDNAITELPVK
                     GWMNKTTRYTASYLSLAQPYGFTTYGSKGNWRSPQFYRTIPGAFGARASKLYALIRTG
                     HHGVRLDPDARQRLIVWLDSVCQFYGVYEKEGGEAQLAGGLARPTLQ"
     gene            complement(3382..4785)
                     /locus_tag="PK576_08635"
     CDS             complement(3382..4785)
                     /locus_tag="PK576_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HOE61697.1"
                     /translation="MNQNITPKCSSWFRAVFRVAAIAFPLALRAESEQQSPMDLGVFF
                     TAPDSWSLTAETFMGQHATFGFRFVDGRQTVMSNQKEVLRFLGLPLYEARVYFTPEGV
                     RRVELSIYNKGDAGVRDQEAFEAEVATVKEKLSAALQDTGMTGRTSSDRANYRIWRHQ
                     WTRKTPGVQLEWACVEPHRSGGQNVPYSAEFITVLLVPIRKGGGGMTAVTGGMTAARA
                     KNARTIRENVVRAPEGDVRIDNVPMVDQGQKGYCAAAASERVLRYYGLQVDQHQIAQL
                     ADTAAEGGTTLEGMAKAIAKVGRQFQLDKKELIAPDAEVHFEKSEHLRLIEQYNVAAR
                     KRKAAMIEWKDFLTDNRVDLPAIWRTMDPAILLAARTSQRQAMNQFVRHIKTYTDQGV
                     PLLWSCLVGLYPEDPPLGENGAFGHVRLILGYNEKNGEVLYSDSWGPRHALKRLPLDQ
                     AWAMTKGLIVLKPRGIR"
     gene            complement(4844..6139)
                     /locus_tag="PK576_08640"
     CDS             complement(4844..6139)
                     /locus_tag="PK576_08640"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexokinase"
                     /protein_id="HOE61698.1"
                     /translation="MRNREELFLAENGVNVEGIDREALLAVFDEEMAAGLAGRPSSLM
                     MIPSFLSIGQPVRTGTPVVVLDAGGTNLRAAVVALDAAGRPEISAFRKRSMPGTEGEL
                     EAEAFFDAFAEFLMPVIDAAETIGFCFSYAAEILPDCDARLIRWSKQIQAPSVEGQRI
                     GAGLKRHLARRGYTRRIVILNDTVATLLAGRSMGVTHDYSAYVGFILGTGTNTAYVER
                     NARITKRTDLDPAGSMVINVESGAFRRVTQSRFDSLMDAETRDPGSYTFEKMISGAYL
                     GSLGLTVLREARKAGLFQPAAAAAIDAWPSLSNKHLDDFCGGRPASDNPFLDAVFTAE
                     DRETVVRLATPLYARAAVLTAVNLASAILKTGEGRDPARPVCVNVDGSTYYRTLTADF
                     RNLVGRELAALLEPRGIAYELIRVEDSPVIGAAVAGLMG"
     gene            6280..6651
                     /locus_tag="PK576_08645"
     CDS             6280..6651
                     /locus_tag="PK576_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOE61699.1"
                     /translation="MTNSLEDYLETLYLLAQANGHARVRDVAAALHVKMPSVIKAVLE
                     LKKKGLVVQEPYGAVELTAAGVRAASAILGRHTLLKTFLSLIGVSEENAEKDACSMEH
                     FLSAETLARVTEFVRKHKRKT"
     gene            6671..7033
                     /locus_tag="PK576_08650"
     CDS             6671..7033
                     /locus_tag="PK576_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61700.1"
                     /translation="MSHRSVRVLLLLLLLPFFRASASDPGTALQTATAVKGSTPGLMR
                     AGRGLAEVPRQAAQCFRLPLGLTQMLFSPFPEVTFSEGLRNTGRGVVAPFRFCIAVLE
                     MPYEVVGGLGDAVSGALP"
BASE COUNT         1318 a         2379 c         2188 g         1261 t
ORIGIN      
        1 tcctgatttt catgaagttt catgtgtttc agggtttcat ccaaatcggg gtcggaatcg
       61 ctatcgctat cgggatcggc tgtttcacca cgggacactt tcttgggttt ctggttgcca
      121 aggggcggcc aggtctttta aactaacagc acgaaagcga ggttcaagtg attctaccga
      181 tcgtgacatt cggacatgat gtgctcaggc aaaaggcgac gcctgtgccg gaggtcaacg
      241 aggacgtgcg gcagctcgtg cgggacatgc tcgaaagcat gtacgccgcc aaaggcctcg
      301 gtctggccgc tgagcagatc ggccgcacgg agagcgtctg cgtcatcgat gtgccgaagg
      361 aggcggagaa agaggagtgc cgtgaggaga acgccgcgat ccccatgccg ctgatcctga
      421 tcaaccccga aatcaccgcg acccaaggcc ggcaacgcaa tgaggaaggg tgcctcagtt
      481 ttccggagat cggcgcgccc atcacccgcg ccgaccgggt gaccgtatcg ttcacggatc
      541 tcgacgggaa ccgtcaaacc ctcagcgcgc gcggtctgct ctcgcgggcg atccagcacg
      601 agactgacca tctgaacggc gtgctgctgg tggaccggat gtcgcccctg cagcgcatgt
      661 ccgtttccgg tcagctcaaa cgccttcaaa aagccgcgca aaccgaatga ccggccaagt
      721 ccccggtcac cggtgacgcg gctttccatg cggtaaccaa aagaaaaaaa tgaaactgat
      781 gaacctgatg ccgctttgcg gagcgttcgc gctatgcgcc ctgacctgct ccgcctctga
      841 ttggctctcg cccgccgcgc cgggacgcgc ctacccggaa gacgaggccg accgtcaatg
      901 ggccgtcctc tcttcggatc tgacccggaa agcccgtttc gaccaattcg ccgccgagac
      961 ggtctgtgcc gaagcgctca tcaccgcaac cgaccgcgac ccgctcgata ttctgctgcg
     1021 ccgcacagcc gcgctcctcg cggacctgcg gcgccagacg ggtgtccggg atttaactcc
     1081 tgaaagccgg gaactcgccg cccttcgcga acaggcggaa accacaaatc cggaacaggt
     1141 tgccgcgcgc cgcgcgtttt tcgaccgtgc gctgcggctg cgccgccgca tcgccttcgc
     1201 caatccgctg ctcgacttcc gcgaaatgct cttcatcaaa cgcgaactgc gcggcgtgat
     1261 ggagcactgc tgcgatcagt tttacgggca gcagcagcgc tcgggcgggg gcctgttcgt
     1321 tctggccgat cccttcggcg accgccccgc gctacgcgac atgttggcga acgcgcgcgt
     1381 gacaaatggg ccgctcaaag gacagcccct tgccttcggc acacgcgcga cgcaggggaa
     1441 ggacggcggt tcgatcgtct cgcctgcgct cgactatgac ggcaagcgta ttgccttcgc
     1501 gtatgtggag ggcgtcggct cgcgcaaaca catccgtcac ctcgaccatg cgcgtgacgg
     1561 ccactgggag cgcggcttct gttaccatat cttttcggcc ggtaccgacg gcacacgcct
     1621 cacgcaactc acggacggca ccttcaacga cttccagccc tgtttcacgc cggccggccg
     1681 aatcgccttc atctccgagc ggcgcggcgg atatctgcgc tgcggccggc actgccccgc
     1741 cttcacgctg ttcgatatgg cggaggacgg ctcggatatc cgttgcctga gttttcacga
     1801 gaccaacgag tggtcgccag ccttcaccca cgacggcatg ctgctctgga cgcgctggga
     1861 ctatgtggac cgccacgggt gcgtggccca caaaccgtgg ttgaccaccc ccgacggccg
     1921 caacccgcgt cccgttcacg ggaattactc gctccgcccg ttgcggcccg atatggaact
     1981 cgacacccgt cccatcccgg aatcccacct ccttctggcc accgccgcac cgcaccacgg
     2041 acaggccttc ggctcgctgg tgctgatcga cccgcgcgcg gaagacgatg acgccatggg
     2101 gccggtgcgc cgcctgaccc cggacgccgg tttcccggag agccaaaaag gttcgcagac
     2161 ctacggcacc ccctggcctt tgagcaagca ttacgcgctc tgcgcctaca ccccggtgga
     2221 ggcgctcggc accgggcagc gtcatctctt cgggctctat ctgctggatt gcttcggcaa
     2281 caaggagctg atctaccgtg accccggcat cgcctgcctc aaccccatac cgctccgcgc
     2341 ctccccccgt ccgccggtgt tgccggaaca gcggcagcgc atcgcgtccg gcgaacgcgg
     2401 cgaagccacc gtgatgatcg ccgacgtcta caagagccag atgccgtggc ccgaaggcac
     2461 ggttatcacc gcgttgcgcg tttggcagat ctttcccctc tccgtggctt cggcggaggt
     2521 gacacacaac atcggcatcc agatcatcga gggccacgac tcgatcaacc tcgcgcgggt
     2581 cgtcctcggc acgacgccgg tcgagaagga cggcagcgca cacttcaccg tacctgcggg
     2641 cgtcgaactc tttttccagg cgctcgatgc cgacggttgc gcgattcagt ccatgcgctc
     2701 ggccactgcg ttcatgccgg gggagcgcgc atcctgtcag ggctgccacg agcccaaagg
     2761 cggtgccccc gaacctgcgc cgaagaccgt caccctggcc atgcggcgcg cgccttccgc
     2821 gctcgttccc ggccccgaag gctcgaaccc gtttagcttt ccccggctgg tgcagcccgt
     2881 gctggatcgc tactgcgtgg ggtgccatgc ggaaaccctc cgcaccgcga aacccggcgc
     2941 gaaaatgccg ccccgcctgg acaacgccat aaccgaactg cccgtgaagg gctggatgaa
     3001 caagaccacc cgctacaccg cgtcatatct gagccttgcc cagccctacg ggttcaccac
     3061 atacggatca aaggggaact ggcgctcgcc gcaattctac cgcaccatcc ccggcgcgtt
     3121 cggtgcgcgc gcttcgaaac tgtatgccct gatccggacc ggccatcacg gggtccgcct
     3181 tgatcccgac gcgcggcaac gcctgatcgt ctggctcgac tcggtctgcc aattctacgg
     3241 cgtgtatgag aaagagggcg gcgaagccca actcgcgggc ggactcgccc ggcccacgct
     3301 ccaatgaaca gaagaggggg aggggggaag agagcataaa tcgcaggccg aatgtcgcgg
     3361 gtcgcaggcc gacttgccgg ttcacctgat cccgcgtggt ttcagcacga tcaggccctt
     3421 ggtcatggcc catgcctgat caaggggcag gcgcttgagc gcatggcgag gcccccatga
     3481 atccgaatag agcacctctc cgttcttctc gttgtacccg aggatcagac gcacgtggcc
     3541 gaacgcgccg ttctcgccga gcggcggatc ttccggatag aggccgacaa gacaggacca
     3601 gagcagcggc acgccctgat ccgtgtacgt tttgatgtgc cgcacaaact ggttcatggc
     3661 ctgacgctgg ctcgtccgcg cggccagcag aatggcgggg tccatggtcc gccagatcgc
     3721 gggcagatcg acccggttgt cggtgaggaa atccttccac tcgatcatcg cggccttccg
     3781 cttccgcgcg gcaacgttat actgttcgat cagacgcaag tgctcgctct tctcgaaatg
     3841 gacctcggcg tccggcgcga tcaactcttt cttatcaagc tggaactggc ggccgacctt
     3901 ggcgatggcc ttggccatac cctccagcgt ggtgcccccc tcggccgcgg tatcggcaag
     3961 ctgggcgatc tgatgctgat cgacttgcag tccgtaataa cgcagcacgc gctcagaggc
     4021 ggcagcggcg caataccctt tctggccctg gtcgaccatc ggcacgttgt cgatccggac
     4081 atccccctcg ggagcgcgca cgacattctc ccggatggtc cgggcgttct tggcgcgcgc
     4141 ggcggtcatg ccgccggtga ccgcagtcat gccgccgccc cccttgcgga tggggaccag
     4201 caggaccgtg atgaactcgg cgctgtaggg gacattctga ccgccggagc ggtgcggttc
     4261 cacgcaggcc cactccaact gcacccccgg ggttttgcgg gtccactggt gccgccagat
     4321 cctatagttc gcccggtcgc tggatgttct gccggtcatg ccggtgtcct gcaaagcggc
     4381 ggaaagcttc tctttgaccg ttgccacctc cgcctcgaaa gcctcttgat cccgcacacc
     4441 ggcgtcacct ttgttataga tcgaaagctc gacgcgccgc actccctccg gggtgaaata
     4501 gacgcgcgcc tcgtaaagcg gcaacccgag aaaacgcaaa acctccttct ggttggacat
     4561 cacggtttga cggccgtcca caaaacggaa tccgaacgtc gcatgctgac ccatgaacgt
     4621 ctcggcagtc agggaccagc tgtcgggggc agtgaaaaac acgcccaggt ccatcgggga
     4681 ctgctgttcc gactcggcgc gcaaagccaa cggaaacgcg atggccgcaa cacgaaaaac
     4741 ggcccggaac catgaactgc acttcggagt gatgttctgg ttcataatac gatcctttct
     4801 aaaccgagcg gcagagccgg tgcggcaggc acctgcccgc atctcagccc atcagtccgg
     4861 ccaccgccgc gccgatgacg ggcgagtcct ccacccggat gagttcgtag gcgatcccgc
     4921 gcggttcgag cagcgcggcc aactcacgtc ccacaaggtt acggaaatcc gcagtcagcg
     4981 tgcggtaata ggtggaaccg tccacattaa cgcagaccgg ccgcgcggga tcgcggcctt
     5041 cgccggtttt gaggatggcg gaagcgaggt tcaccgcggt cagcacagcc gcacgcgcgt
     5101 agagcggcgt ggcgagccgt acgacggttt cgcggtcctc cgcagtaaaa accgcatcaa
     5161 gaaacggatt gtccgaagcg ggtcggcctc cgcagaaatc gtcgagatgc ttattcgaga
     5221 gcgacggcca ggcatcgatc gcggcagcgg cggcaggctg gaagagcccc gctttccgcg
     5281 cttcgcgcaa aaccgtaagc ccgagcgaac ccagataggc gccggagatc attttctcga
     5341 aggtgtagct gccggggtcg cgggtctcgg catccatcag actgtcgaac cggctctggg
     5401 tcacccgacg gaacgcgccc gactcgacat tgatcaccat cgaccccgcg gggtcgagat
     5461 ccgtgcgttt ggtgatgcgc gcgttgcgct ccacgtaagc cgtgttggtg ccggtgccga
     5521 gaatgaaccc cacataggcc gaatagtcat gcgtgacccc catgctccgg cccgcgagca
     5581 gcgtggcgac cgtgtcgttc aggatcacga tacgccgcgt gtatccgcgc cgcgccagat
     5641 ggcgttttag gccggcgcct atccgttgcc cctccaccga gggggcctgg atctgcttgc
     5701 tccagcggat caggcgcgcg tcgcagtccg gcaggatttc agcggcatag gagaagcaga
     5761 aaccgatggt ctcggcagcg tcgatcaccg gcatcaggaa ttccgcgaaa gcgtcaaaaa
     5821 acgcctcggc ttcaagctcg ccctctgtgc cgggcatgga gcgtttgcga aaggcgctga
     5881 tctccggccg gcccgcagcg tccagcgcca cgaccgcagc gcgcaggttc gtgccgcccg
     5941 catccagcac caccaccgga gtgccggtcc ggaccggctg tccgatcgag agaaaagagg
     6001 ggatcatcat cagcgaggac ggcctgcccg ccaacccggc ggccatctcc tcgtcaaaca
     6061 ccgcgagcag ggcttcgcga tcaataccct cgacatttac cccgttttcc gcgaggaaca
     6121 gctcttcacg attgcgcatg ggcttctccc gtacattctg tttgagatag aatgctattt
     6181 caacgctccc cggcgttttt gtcaatgcgg aattcggaca cgcaaggaat cgcgaaaaag
     6241 cctttgccga aacccgttcg accgtgtatg gtagggacga tgacgaacag tcttgaggat
     6301 tatctggaga cgctttatct gctggcgcag gccaacgggc acgcacgcgt tcgggatgtc
     6361 gcggcggcgc ttcacgtcaa gatgccctcc gtgatcaagg cggttctcga gctcaagaaa
     6421 aaagggcttg tggtgcagga gccctacggg gcggtggaac tgaccgccgc gggcgtgcgg
     6481 gcggccagcg cgatcctcgg ccgccacacg ctgctgaaga ccttcctctc gctgattggg
     6541 gtttcagaag agaacgcgga gaaagacgcg tgttccatgg agcacttcct gagcgccgaa
     6601 accctcgcgc gcgtcacgga attcgtgcgc aagcacaaac ggaaaacctg agtccggaca
     6661 aaggggcggc atgtcacacc gcagtgtcag agttctcctc cttctcctcc tcctgccgtt
     6721 cttccgcgcg agcgcgtccg atcccggcac ggccctgcaa accgcgaccg cagtcaaagg
     6781 cagtacgccc ggactgatgc gcgcgggccg cggccttgcc gaggtgccgc gccaggcggc
     6841 gcaatgtttc aggctgccgc tcggcctcac gcagatgctc ttctcgccgt ttccggaggt
     6901 gaccttctcc gaagggttgc gcaacaccgg ccgcggcgtg gtcgcgccgt tccggttttg
     6961 catcgcggtg ctggaaatgc cgtatgaggt ggtcggcggg ctgggcgatg ccgtaagcgg
     7021 cgcattgccc tgagaaggag ccggcctttg cgccgtagaa ggcggggcgg ttcttgagtt
     7081 tcgtggcgtc atcctcaagc ggggacttga agagcgccgc gtggacgacg tagatccgga
     7141 gcggca
//
LOCUS       DAOSLE010000134         7120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9582_length_7120_cov_8.563340, whole genome shotgun sequence.
ACCESSION   DAOSLE010000134 DAOSLE010000000
VERSION     DAOSLE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9582_length_7120_cov_8.563340"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="PK576_08655"
     CDS             <1..1099
                     /locus_tag="PK576_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE61701.1"
                     /translation="VYKTKLDKPGFVRLYAEVIGKDGKRYQKVFTGDTSTPEGREAAN
                     RFERQNKSIFFDGGAGAETCTLQALPEPEDFDAFWDRQRARLAKIPMKAERIELPCNN
                     KDARIYAITIDCAGIRPVTGYLTIPKAADNGAKFPCRLTTHGYSYRAPHNPPGGARTD
                     EIVLDINAHGMRLPAFGGDEGYYKALGSEIKSNGQSYAFDPVENKDPETAYFNGMALR
                     VMRALEYLKSLPGWDGKNLIASGGSQGGLQTIWAAGLDKDVTHAESSITWCCDLGGER
                     LGRNRGGWYIKWSDGLGYYDAVNVAKRIPTSCRTIIPRAGLGDYVCPPSGLAILWNNI
                     KGPKKITWVQGSQHGYVPPEKYEGRDFEMEK"
     gene            1133..3238
                     /locus_tag="PK576_08660"
     CDS             1133..3238
                     /locus_tag="PK576_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61702.1"
                     /translation="MKKLVSGIFVMFCATVVCATDCSVYGNEKPYDFRARLAHVHKPN
                     RRDWKLQPTANEFVFLDGMKIAVGENAAPLVSRVADDFRDYLLTSMGVNVSVHEAGKP
                     KKPEDCAVKVKIDKAMKEREYAIEVGEKGVTIRAADAKAAAQGFYRLEDRMNLRSAPF
                     LAKGSETRRPIFSPRMSHSGWGCDQFPDAHLAQMAHYGIDAILIFVEDVDVTKNVAHI
                     DIADTIRRAKAWGLETYLYSYVLAFAHPDDPGAKELFEKTYGRVAAAYPEAKGIIFVG
                     ESCQFPSKDERALPYCYWEKDKIAAHRKKGDRRPLAGWFPCRDYPDWLNAVKAAIRAQ
                     APNMEIVFWSYNWGYHDEKERLALLNALPRDVTLQATFEMFEKFTKRNGLPSRVADYS
                     LTYVGPGNYFASEAKEAQKLGMKMYAMSNTGGHTWDFGTIPFDPFPYQWKKRFDAVVK
                     AHVDWNLAGLMENHHYGWWPSFISELSKETYTEGGMPFETHIRAIAARDYGEKNVDAV
                     LAVWKDWSEKIVDMHSESENQYGPFRYGPAYPFNAMEPDLEASELPFPPMYLTLNYRY
                     SAAKNESLTLQIELLDPMVESFMSGGNTFLALAAKETDATRRDNARRLGWHGQYMARC
                     CLTARNVKAAVIAERAKDRAKVVEIARAEYANAKAALKLLHADSRLGWEPTMLYRGGP
                     EMIEWKLRYMEEHYGIGDQ"
     gene            3293..3817
                     /locus_tag="PK576_08665"
     CDS             3293..3817
                     /locus_tag="PK576_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOE61703.1"
                     /translation="MKTQKLEVVEVSKLVKTPGDSIMKRRTVLSAFLLLAAVSTALGF
                     APRTYDVTSPSMGKAVPVTIVLPKGYEDDPARRHPVVYLLHGANDTDFHAYHPLFRKA
                     ADENGVILVAPCGTLSWWMDSPVDPKHRYETFVTKELIPWVDARYRTIPDRMHRGLAG
                     NSMGGLFWYVWRAA"
     gene            complement(3822..4070)
                     /locus_tag="PK576_08670"
     CDS             complement(3822..4070)
                     /locus_tag="PK576_08670"
                     /inference="COORDINATES: protein motif:HMM:NF039850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5763 domain-containing protein"
                     /protein_id="HOE61704.1"
                     /translation="MKPTMKRSVITLAAALIAAAALAASVQCRGTTKRGARCKNKTTD
                     ASGYCHLHKSQAYRHASAAATNGTRRAHGVAATNSLPR"
     gene            complement(4132..5241)
                     /locus_tag="PK576_08675"
     CDS             complement(4132..5241)
                     /locus_tag="PK576_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61705.1"
                     /translation="MKMANQTRGPFAASPLMPRSLWDKLLFRENPGNAYVELVNALAR
                     TDDIRSIPEDFADTLNRKYRTNLHSRRRADLDRIYADFLRHCLADLAFTAREAEEANR
                     LRRLFLITDAKHAKIFSSVADETHRRAVKRVIADGKVTDNERRALEYLAASLLISERT
                     SKAIYKDEVDKMLQGRLDAAVSDCMLSPQEEAELQELAAGLGVELTHSAATARTLDSY
                     RAMWRVRYGDLPTLDSGINLRRGEVCYMKRAVEWHEMRRQRVGATYSGPTMRLKIAKG
                     VYWRTGTLGIKPVTRDTLVKIDSGHAYITNKRLLFTGAMKNTSIPLNRILDITKYSDG
                     VGIEKDAGKSPVLVFHEGIDIFCAVLSRVISEQAP"
     gene            complement(5238..5495)
                     /locus_tag="PK576_08680"
     CDS             complement(5238..5495)
                     /locus_tag="PK576_08680"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE61706.1"
                     /translation="MREKRGNKPQSQMAKELGMKQPMHARYESGMTLPSAELLFHICS
                     KLGVSADWLLGLSDDETGRQPATVAGGGSRVNEKAKERKEA"
     gene            5777..6868
                     /locus_tag="PK576_08685"
     CDS             5777..6868
                     /locus_tag="PK576_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61707.1"
                     /translation="MTNAMNATTLFGDGSIRIGGFSKFIINGQAYRCGLNPSTADGLL
                     DFVVYDNGQFDASLEDATYLNNSALAGSVTRITASDSATVKFGHNAVVGYAAGSTGEL
                     ILKDSVTCQNGLYGFTFGRGGSATDISTGILRMSGGTFTIGSSKTTTYPDLYGFVFGN
                     GAATNATGEIWNKGFGYISGGTITVCQGIHIGDGNAYGFVEQSGGKIQMSNNNSYPVN
                     VGNHHGTGVYRMTGGTFHTPNSTVSVGANNGNGMLEFGAGTGTCTAKNMNLAGVGATL
                     KFVFGADGSVIKPNVTDTLTIDPGAKIVVDTTACAEPKSVDLMTFGAKSGSFADADIE
                     IIGIKGALVSQSDAKLRLQINKGTVVIIR"
BASE COUNT         1468 a         2034 c         2191 g         1427 t
ORIGIN      
        1 tgtctacaag acgaaactcg acaagccggg gttcgtgcgg ctctatgcgg aggtcatcgg
       61 caaggatggc aaacgatacc aaaaggtctt cacaggtgac acatcgacgc ccgaaggccg
      121 ggaagcggca aatcgcttcg agcgtcagaa caagtcgatc ttttttgacg gcggcgcggg
      181 cgcggaaacc tgtacgcttc aagcgcttcc ggagcctgaa gatttcgatg ccttctggga
      241 tcgtcagcgt gcgcggctcg cgaaaatccc catgaaagcg gagcgcatcg aactcccttg
      301 caacaacaag gacgcgcgga tctacgcgat caccatcgat tgcgcgggga tacgtccggt
      361 gaccggctac ctgaccattc cgaaggccgc cgacaatggc gccaagttcc cgtgccgctt
      421 aacgacccac ggatattcct acagagcgcc gcacaatccg cccggcggcg cgcggaccga
      481 tgaaatcgtc ctcgacatca acgcgcacgg catgcgcctg cccgcgttcg gcggcgacga
      541 aggatattac aaggcactcg gctctgaaat caaatcgaac gggcagagct atgcttttga
      601 tccggttgag aacaaggatc cggaaacggc ttatttcaac ggcatggcac tccgtgtgat
      661 gcgggcgctc gaatacctga agtcgttgcc gggatgggac gggaaaaacc ttatcgcgtc
      721 cggcggcagc cagggcgggt tgcagaccat ttgggcggcc gggctggaca aagacgtcac
      781 acacgcggag tcctccatca catggtgctg cgatctcggc ggcgagcgcc tcgggcgcaa
      841 ccgcggcgga tggtacatca aatggtccga cgggctggga tattacgatg cggtcaatgt
      901 cgccaagcgc attccgacat cctgccggac gatcattccc cgggccggac tcggcgacta
      961 cgtctgcccg ccatcgggac tcgcgattct ctggaataac atcaaaggcc cgaaaaaaat
     1021 cacctgggta cagggttcgc aacatggtta tgtcccgccg gaaaaatacg aaggccggga
     1081 ctttgagatg gaaaagtagg ctcacacacc acaaagaaag gttaggcgaa tcatgaaaaa
     1141 actcgtttcg ggaatcttcg tgatgttttg cgcgacggtg gtctgcgcaa cggattgtag
     1201 cgtatatggc aacgagaagc cctatgactt ccgtgcgcgg ctcgcgcatg tgcacaagcc
     1261 gaaccggcgc gactggaaac tccagccgac cgcgaacgag ttcgttttcc tagacggcat
     1321 gaagatcgcc gtgggcgaaa atgccgcgcc gctcgtcagc cgcgttgccg atgatttccg
     1381 tgactacctg ctcacttcaa tgggtgtgaa cgtttcggta cacgaggcag ggaagcctaa
     1441 aaagccggag gattgcgccg tcaaggtgaa gatcgacaag gccatgaagg agcgggagta
     1501 cgcgattgag gtgggagaga agggcgtgac gatccgcgcg gcggacgcga aggcggcggc
     1561 acaaggcttt taccgtctcg aagaccgcat gaacctccgc tcagcgccgt ttctcgcgaa
     1621 aggctcggaa acgcgccggc cgatattctc gccgcgcatg tcgcattcgg gctggggctg
     1681 tgatcagttt cccgacgcgc acctcgcgca gatggcgcat tacggcatcg acgcgatcct
     1741 tattttcgtg gaggatgtgg atgtgacaaa aaacgttgcg cacatcgata tcgccgatac
     1801 gatccgccgc gcgaaggcgt gggggctcga gacctatctc tacagctatg tgctcgcctt
     1861 tgcacatccc gacgatccgg gcgccaagga acttttcgag aaaacctacg gccgcgtggc
     1921 cgccgcgtat cccgaggcga agggcatcat cttcgtgggc gagagctgcc agttcccctc
     1981 aaaggacgag cgcgcactcc cctactgtta ctgggagaag gacaagattg ccgcccaccg
     2041 caagaagggc gatcgccggc cgctcgcggg ctggttcccg tgccgcgact atcctgattg
     2101 gctcaacgcc gtcaaggcgg cgatccgcgc gcaggcgccg aatatggaga tcgtgttctg
     2161 gagctacaac tgggggtatc acgatgagaa ggagcgtctg gcgctcctga acgcactgcc
     2221 gcgcgacgtc acgttgcagg cgacattcga gatgttcgag aagttcacca agcgcaacgg
     2281 cctgccctcg cgtgtggcgg attattcgct cacctatgtg ggaccgggga actactttgc
     2341 ctctgaggcg aaggaggcgc agaagctcgg gatgaagatg tacgccatgt cgaacaccgg
     2401 cggccataca tgggacttcg gcacgatccc cttcgatccc ttcccgtacc agtggaagaa
     2461 gcgtttcgat gcggtcgtca aagcgcacgt cgactggaac ctcgccggtc tcatggagaa
     2521 ccaccactac ggctggtggc caagtttcat ctcggagctc tccaaagaga cttacacgga
     2581 gggcggcatg ccgttcgaga cgcatatccg ggcgatcgcg gcgcgtgact acggcgaaaa
     2641 aaatgtggat gcggtactcg ccgtgtggaa agactggtcg gagaagatcg tggacatgca
     2701 ctccgagagc gagaaccagt atgggccgtt ccgctacggc ccggcctacc ccttcaatgc
     2761 gatggaaccc gacttggagg cttcggaact gccgttcccg ccgatgtatc tgaccctgaa
     2821 ttatcggtat agcgcggcaa agaacgagtc gctcacgctg cagatcgaac tgctggatcc
     2881 tatggtggaa tccttcatga gtggcgggaa cacgtttctt gcgttggcgg cgaaggagac
     2941 ggacgcgacc cgacgggaca acgcccgccg cctcggctgg cacggccaat acatggcgcg
     3001 ttgctgcctc acggcacgca acgtcaaggc cgccgtgatc gccgaacgcg cgaaagaccg
     3061 cgcgaaggtg gtagagatcg cccgtgccga atacgcgaat gcaaaggctg cgctcaagct
     3121 tctacatgct gattcacgcc tcggctggga gcccacgatg ctctaccgcg gcggccctga
     3181 aatgatcgaa tggaaactcc gctacatgga agagcactac ggtatcggag atcagtaaac
     3241 ctaaaaagag cagttgaact ttcgtggaaa gcgatgaaaa gaaagtgacg gcatgaagac
     3301 ccaaaaactg gaagtcgtgg aggtgtccaa actcgtgaag acgcccggag attcgatcat
     3361 gaagcgccgg acggttctgt cggccttttt gttgctggcg gcggtatcaa cggcccttgg
     3421 ttttgctccg cggacctacg atgtcacgag cccctcgatg ggcaaggccg ttcccgtgac
     3481 aatcgtcctg ccgaagggct atgaggacga tccggcgcgg cgtcatccgg tcgtctacct
     3541 ccttcacggc gcgaacgaca ccgatttcca cgcataccat ccgctattcc gcaaggcggc
     3601 ggatgaaaac ggcgttatcc ttgtcgcgcc gtgcggcacg ctgagctggt ggatggacag
     3661 tcccgtagac cccaagcacc ggtacgaaac atttgtgacc aaggagttga ttccgtgggt
     3721 cgatgcgcgt taccgcacga ttcccgaccg catgcaccgg ggcttggccg gcaactccat
     3781 gggcggcctt ttttggtatg tctggcgggc ggcctgacct gtcagcgcgg cagcgagttt
     3841 gtcgcggcaa ccccgtgggc gcggcgggtg ccgttcgtcg cggcggcgga cgcgtgcctg
     3901 taggcctggc ttttgtgcag gtggcagtag ccggaggcgt cggtggtctt gttcttgcac
     3961 cgcgcgcccc tcttcgttgt ccccctgcac tgcacggagg cggcgagcgc cgccgccgcg
     4021 atcagcgcgg cggccagcgt gatgacgctt cgcttcattg tcggcttcat gcttctttcc
     4081 tttccttcct tttgtgatca cacgccccgc atgcgcgcgg ctgtttttgt ttcacggcgc
     4141 ctgctcggag atcacgcggg agagcaccgc gcagaagatg tctatgcctt catggaagac
     4201 gaggacgggg ctcttgccgg cgtctttctc tatgcccacg ccgtcgctgt atttggtgat
     4261 gtcgagaatc cggttcaggg ggatgctggt gtttttcatc gcccctgtga agagcaggcg
     4321 cttgttcgtg atgtaggcat ggccggagtc gatcttcacc agggtgtcgc gcgtgaccgg
     4381 cttgatcccc agcgtgccgg tccgccagta cacccctttt gcgatcttga ggcgcattgt
     4441 cgggccggag taggtcgcgc cgacccgctg ccggcgcatt tcgtgccact ccacggcgcg
     4501 cttcatgtag cagacctcgc cccgccgcag gttgatgccg gaatcgagcg tggggagatc
     4561 tccgtaacgc acgcgccaca tcgccctgta ggaatcaagg gtgcgcgccg tggcggcgct
     4621 gtgcgtaagc tcgacgccga ggccggcggc cagctcctgt agctccgcct cttcctgcgg
     4681 cgacagcatg cagtctgaca cggccgcgtc gagccgcccc tgaagcattt tatccacttc
     4741 gtctttgtag atcgctttac tggtacgctc cgatatgagt aacgacgcgg ccaaatattc
     4801 gagtgccctg cgctcattat ctgtgacctt gccgtccgca ataacccgtt ttaccgcgcg
     4861 acgatgcgtt tcatctgcga cagagctaaa aatcttggcg tgctttgcgt ctgtaatcag
     4921 gaagaggcgg cgcaggcggt tggcctcttc cgcctctctg gccgtgaagg cgaggtcggc
     4981 caggcagtgg cggaggaaat ctgcatagat ccggtctagg tctgcgcggc ggcgtgagtg
     5041 caggttcgtt ctgtatttgc ggttgagggt gtctgcaaag tcttctggga tggagcggat
     5101 gtcgtctgtc cgggcgaggg cgttcaccag ctcgacgtag gcgttcccgg ggttttcacg
     5161 gaagaggagc ttgtcccaga gggagcgggg cataagcggg gacgcggcga acggcccgcg
     5221 cgtttggttt gccatcttca tgcctctttc ctttccttag ccttttcgtt caccctggag
     5281 ccgccaccag cgacggtcgc gggttggcgg cccgtttcgt catctgacag ccccaaaagc
     5341 caatcggcgg acacaccgag ttttgagcag atgtgaaaca gcaactcggc actcggtagc
     5401 gtcattccgc tttcatatcg tgcatgcatt ggctgtttca ttccaagctc tttcgccatt
     5461 tgcgattgcg gtttgttccc ccgtttttca cgcaaacggg cagagaaaac atttttgtca
     5521 aaaaaactcg cataataaca cttagttatt ggcaggttaa taactgacta gttattatcc
     5581 ccgctgtaac ggcaacgtgc cacgtaggaa aggtagcaaa tgaagatgag cgggaaaagg
     5641 aaatcgtcaa tccggcgtga gacgcgcagc gtgattccgg ggatatgccg ggacgcgcgg
     5701 cggcacggcg tctctcggat gcacctgtgg gcggttctta cggggcggcg cgatcgatct
     5761 ttccggtgac gctgtcatga ccaacgccat gaatgccact acgcttttcg gcgacggttc
     5821 gatccgcatc ggcggtttct cgaagttcat catcaacggc caggcctatc gttgcgggtt
     5881 gaatccgagc acagcggacg ggcttctgga ttttgtcgtc tacgacaatg gacagtttga
     5941 tgcgtcacta gaggatgcga cctatctcaa caactcggct ctcgccggga gcgtgacgcg
     6001 gattacggca tccgactcgg cgacggtgaa attcggccat aacgcagtcg tcggttacgc
     6061 cgccggatcg acgggcgagt tgattctgaa ggactcggtg acctgtcaga atgggctgta
     6121 tggtttcaca ttcggaagag gcggttccgc gactgatatt tcgaccggca tcctcaggat
     6181 gtccggcggt acgttcacaa tagggtcgtc aaaaaccaca acttatccag acttatatgg
     6241 gtttgtcttc ggtaacggcg cggcgaccaa tgcgacggga gagatctgga ataagggctt
     6301 cggttatatc tccggcggca cgatcacggt ttgccagggc atccatatcg gtgacggcaa
     6361 tgcctacgga tttgttgagc agtcgggcgg caaaattcag atgtcgaaca acaacagtta
     6421 ccccgtcaat gtcggcaatc atcacggtac cggcgtgtat cgcatgaccg gcggcacgtt
     6481 ccacacccca aattccacgg tcagcgtcgg cgcgaacaac ggcaacggca tgttggagtt
     6541 cggcgcaggc acaggcacct gcacggcaaa gaacatgaat ctcgcgggcg tgggggcgac
     6601 cctgaagttc gtgttcggcg cggacggttc ggtcatcaag ccaaacgtga cggacacgct
     6661 gaccatcgat ccgggcgcga agatcgtcgt cgacacaacg gcgtgtgcgg aaccgaagtc
     6721 agtcgatctc atgaccttcg gggcaaagtc cggttcgttc gcggacgcgg atatcgaaat
     6781 catcgggatc aagggtgcgc ttgtttcgca gagtgatgca aagttgcgcc ttcagatcaa
     6841 caaaggcact gtcgtcataa tccggtaaga tgccggaaga ggcgcatgtc ctgcgccgtc
     6901 gcggagcaac agaagaaacc gtcgtcccct ccgctgtgcg tagaagtgcc ggcacattca
     6961 ccggcgcggg cggtttcacg cccaccgcac ccgttttaca aacagcggaa cagaccagag
     7021 ttgaaagctt gaacctaaaa ggagcagttg aacgggtaga attctagcaa agccccgcaa
     7081 acattgggtt ttgcggtgcg cccgacgctc tttttgaact
//
LOCUS       DAOSLE010000135         7097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9625_length_7097_cov_9.649957, whole genome shotgun sequence.
ACCESSION   DAOSLE010000135 DAOSLE010000000
VERSION     DAOSLE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9625_length_7097_cov_9.649957"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(653..3886)
                     /locus_tag="PK576_08690"
     CDS             complement(653..3886)
                     /locus_tag="PK576_08690"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOE61708.1"
                     /translation="MRQFLCMLAGLMVVAGCAQKMWGQHGALAAPQALTVDYLQDPVG
                     LDHAAPRLSWKLIAARRSARDLRQVAYQILVASSPERLRDNQGDLWDSGRVVSDQSLN
                     VVYVGKPLVTSQRCYWKVRVWDNQSDAPTAWSATGRWIMGVMRPNDWQARWIGANAAT
                     RPDCDLAGAQWIWTGDAPSLEQAAQGVRYFRRVFDAPRGAQDAPVLLALTADDEYEVF
                     INGKRAAKTWGHFNESRWMRFQDVSALIKPGRNLIAATVKNKAPGPTALLAVLKFADG
                     QAVPTDTTWSSSAQAVDGWKEKADGFAHDAWKAAVIAGPVDGAPWGKIERRRELVSPA
                     FEKRFTVTRPVREATLHITGLGFYEASLNGQRIGRKVLDPVPTRYDRRVLYSTYDMTE
                     RLTRGENRLHVLLGHGWYDVRSVAVWNFDNAPWRDFPRMLAQLEITYDDGTRTMVVSD
                     DTWRQVASPIGFDCIREGEMIGKAPKGAPDLEKQVLRADVVPAPAGRLTASALPPSVV
                     TQRLKPVAVRAITPGTWLVDFGQNTAGWVKLKLAGQRAGDVVTIKYGEKLKEDGSLSM
                     KGTDAHFRYPASFSTLPDGWFQTDRFVCDGSRTQVYEPRFTYNGFQYVQITGLAQPPD
                     ASNIEACVVHTDFKDAGRFISSNELLNKLQHAILWAYRGNFVNGYPTDCPHREKNGWT
                     GDASLASELAMYNFQNTAAYEKWVQDLIDEQRPDGNLPGIVPTSGWGYQWGNGPAWDS
                     ALVIIPWMLYVYQGDTRVLETAYPAMTKYVDYMTSRAKDGIVSHGLGDWVPVKTKTPI
                     EVTSTGYYYLDAQIVARVAERLGKTADAQKYAALARSIREAYTRHLYKGNGVYSIGSQ
                     TAQSCALHQGLVPATERSTVEARLVEAVQKNNAFPDFGILGSKYVFRALSEAGRTDLA
                     FTMATREEYPSFGNWIKRGATTFWESWKGGSSRNHIMFGDLSAWFYQYLGGIRLADNV
                     SAIAVDADPQAVAFKRFIIAPEPVADLDWVKAEHDSPYGLIRSEWRRENGAFVLEVEV
                     PVNTEATIYLPVKPDAKNVTTDVAPVTSDRDRMAFRVGSGRYRFCAR"
     gene            4086..4226
                     /locus_tag="PK576_08695"
     CDS             4086..4226
                     /locus_tag="PK576_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61709.1"
                     /translation="MEGSRGFSGLLDNFRIYGRILNGQELRNVMLTDDSDVSRRLSIR
                     IH"
     gene            4238..5332
                     /gene="mnmA"
                     /locus_tag="PK576_08700"
     CDS             4238..5332
                     /gene="mnmA"
                     /locus_tag="PK576_08700"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE61710.1"
                     /translation="MENSLPIVAVGLSGGVDSAVTAWLLQQQGHRVIGLTMSIWDPSF
                     GAATAKQSGCFGPGEAENLAAARAIAERLGIPHHTMHLAREYGCGVLDPFRAQHLEGR
                     TPNPCALCNPIMKFGLLPARAREKGIGFDRFATGHYARIVQDGETGLFRLLRGVDPTK
                     EQSYFLARLTQAQLARILFPIGAMAKSEVRETARRAGLGDLTEKPESQDFFEGEDIAA
                     LFHGAGTEPGPITDEAGRVLGQHRGIIRYTVGQRDGLGIAVGHKVYVKTIHADTNTLV
                     VAPREGVFSDRCRVNDFHWIAGEPPQPERALTAHLRYRHKGVSVQITFGPHPETFDLL
                     FAEPQFAVTPGQMAVLYDGDEVLGGGWIAV"
     gene            5470..6159
                     /gene="nth"
                     /locus_tag="PK576_08705"
     CDS             5470..6159
                     /gene="nth"
                     /locus_tag="PK576_08705"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE61711.1"
                     /translation="MTCKDIPAVNRLLKTAFHSMRAPIVDLIAAQTRDPFRVLLGTIL
                     SARTRDACTAEACKRLFARVTTPDALRRIPQDELERLIFPVGFYRDKARHLKALPQVL
                     EQRFGGVLPDTVEELCELPGVGRKTANLVVALGFDKPAICVDVHVHRITNRLGLVNTK
                     TPFETEMALRACLPKRYWKTWNSYLVAFGQTRCLPRNPRCDGCPLATFCPSYAGKKRD
                     APGLPKASRQG"
     gene            complement(6170..6370)
                     /gene="rpsU"
                     /locus_tag="PK576_08710"
     CDS             complement(6170..6370)
                     /gene="rpsU"
                     /locus_tag="PK576_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE61712.1"
                     /translation="MIQLRLKKGESVERALKRLKKIMDKEGLLKQLRNNRYYEKPCEK
                     ARRKSARARIRNNSRRAAREMF"
     gene            6562..6647
                     /locus_tag="PK576_08715"
     tRNA            6562..6647
                     /locus_tag="PK576_08715"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6596..6598,aa:Tyr,seq:gta)
BASE COUNT         1334 a         2188 c         2138 g         1437 t
ORIGIN      
        1 ggccacgggt gaagtcgtcg tgcgcaaggt gcgtctgatt cccaatcgtt aaaggaagga
       61 ccacaaaacc ttcgacgcgc atcgccgttc ccagatcaac ggtaacttca gtaccaccgt
      121 cgacatcctc ggaagggaac gggtggggac atgggaattt cgccccgtgt ccctcccaca
      181 ccatcgaaca tgcggttctc cgcgtcccat gaatgacgcc attccccgag gcacgtcatg
      241 ttgccgtcgg cgtcgtaggt ggggagaatt tggacaggat tgacaggatt tacaggattg
      301 gagatcaggg tgtactggtt gaggcggttg gcggtgtagg tgttggtggc ggcgtagatg
      361 cagagggcgg cgacgagaat taggaaaacc gcccggtcga cgccagacag ggcgcggagg
      421 atgcgccgaa ggcgggcagg gatgcccgcg ttcgggactt ttcacctctg gtttcaaagt
      481 catcctttgg ttttcgcccg gtgcccgggc agccggctta acatcatctg agttccaggc
      541 gcgcgccgtc cgcggcgatc gctacgtatg cggcgcgttt catttttcgt tcccttcccc
      601 ggttttccgg gaacttcgca accgtttttt tagccccgcc gatgatcgat aatcagcgtg
      661 cgcagaagcg ataacggccg gagccgacac ggaatgccat gcggtcgcgg tctgacgtga
      721 caggcgccac atcggtggtc acgttcttcg cgtcgggttt gaccggcaga tagatggttg
      781 cttcggtgtt gaccggaacc tcgacttcca gcacaaacgc gccgttctcg cgccgccatt
      841 cgctgcggat gagaccgtag ggcgaatcgt gttcagcctt gacccaatcc aagtccgcca
      901 ccggctccgg cgcgatgata aaacgcttga aggccacggc ctgcggatcg gcgtccacgg
      961 cgatggcgga gacgttgtcg gccaagcgga taccgccaag atattggtaa aaccaggcgg
     1021 aaaggtcgcc gaacatgatg tggttacgcg acgagcctcc tttccagctt tcccaaaagg
     1081 tggtggcgcc gcgcttgatc cagttgccga aggaagggta ttcttctcgc gtggccatgg
     1141 tgaaggcgag gtcggtgcgg ccggcttcgc tcagtgcgcg aaacacgtac ttcgagccaa
     1201 gaataccgaa gtccgggaaa gcgttgttct tttgtacggc ctcgaccagc cgtgcttcga
     1261 cggtggaacg ttcggttgcc ggaaccaacc cctggtgtag cgcacagctc tgcgcggtct
     1321 gactgccgat cgagtagacg ccgttgcctt tgtagaggtg gcgcgtgtaa gcctcgcgga
     1381 tcgaacgcgc gagcgcggcg tatttctggg cgtcggcagt cttgccgagc cgttcggcaa
     1441 cacgcgctac gatctgcgcg tcaagatagt agtagccagt ggaggtcacc tcgatcggcg
     1501 tcttggtttt gaccgggacc cagtcgccga gaccgtgcga gacaattccg tccttggcgc
     1561 gggaggtcat gtaatcgaca tacttcgtca tagccggata ggcggtttcc agcacacggg
     1621 tgtcgccttg gtagacgtac agcatccacg gaataatgac gagcgcgctg tcccaagccg
     1681 gaccgttacc ccactggtac ccccatccac tggttggcac aatgcccggc aggttgccgt
     1741 cgggacgctg ctcgtcgatc aggtcctgca cccatttctc ataagccgcc gtattttgaa
     1801 aattgtacat cgcgagttcc gaggccaacg acgcatcgcc ggtccagccg tttttttcgc
     1861 ggtgcgggca gtcggtggga tacccgttga cgaagttgcc gcgataggcc cagaggatgg
     1921 cgtgctggag cttgttcaac aactcgttcg agctgatgaa acgtccggcg tctttgaaat
     1981 cggtgtggac tacacaggcc tcgatattcg aggcgtctgg cggctgtgcc agtccggtga
     2041 tctgcacata ttggaaacca ttataggtga agcgtggctc atacacctga gtgcggctgc
     2101 cgtcacacac gaagcggtct gtctggaacc agccgtccgg cagcgtgctg aacgacgcag
     2161 gataacggaa gtgtgcgtcg gtgcctttca tgctgaggct gccgtcttct ttaagttttt
     2221 cgccgtactt gatcgtgacc acgtcaccag cgcgctgtcc ggcgagtttg agtttgaccc
     2281 agcctgcggt attctgaccg aagtcgacca gccacgtgcc gggcgtgatc gcgcgtacag
     2341 cgaccggctt caggcgttgc gtcaccacgc tcggcggcag cgcggaggcg gtgaggcgtc
     2401 cggcgggtgc gggcaccacg tcagccctta agacctgctt ttcaaggtcg ggcgcgcctt
     2461 tcggtgcctt gccgatcatc tcgccttcgc ggatgcagtc aaagccgatc gggcttgcga
     2521 cctgacgcca ggtgtcatcg ctcacgacca tcgtgcgcgt accgtcgtcg tacgtaattt
     2581 cgagttgggc cagcatgcgc ggaaaatcgc gccagggggc attgtcgaag ttccagacgg
     2641 cgacgctgcg cacgtcgtac cagccgtgtc ccagtagcac atgcaaccgg ttttcgccgc
     2701 gcgtgagcct ctcggtcatg tcgtaggtgg agtagagcac gcgccggtcg tagcgggtgg
     2761 gaacagggtc cagcaccttg cggccgatac gctgcccatt gaggctcgcc tcataaaagc
     2821 cgaggccggt gatgtgcagc gtagcctcgc gcaccgggcg agtaaccgtg aaacgctttt
     2881 caaaggcagg cgaaacgagt tccctgcgcc gttcgatctt accccaaggc gcaccgtcca
     2941 cggggccggc tatcacagcg gctttccatg cgtcatgcgc gaaaccatcg gccttttctt
     3001 tccagccgtc cacagcttgc gctgaggagc tccaagtggt gtcggtcggc acggcttggc
     3061 cgtcggcgaa tttcagcacg gcgagcagag cggtgggccc cggcgccttg tttttgacgg
     3121 tggcggcgat caggttgcga ccgggcttga tcaaggccga aacatcctga aaacgcatcc
     3181 agcgcgattc gttgaagtgg ccccaggttt tggcggcgcg cttgccgttg atgaacacct
     3241 catattcgtc atccgccgtg agagccagca gcacgggggc gtcttgtgcg ccacgcgggg
     3301 cgtcgaacac tctgcggaag tagcggacac cttgcgccgc ctgctccagt gagggggcat
     3361 cgccggtcca aatccactgt gcgccggcca gatcgcagtc cggacgcgtg gcggcattcg
     3421 cgccgatcca gcgggcctgc caatcgttcg gacgcatcac gcccatgatc cagcgtcccg
     3481 ttgcactcca ggcggtgggc gcatcgcttt ggttgtccca gacgcgtacc ttccaataac
     3541 agcgctgcga cgttacgagc ggctttccga cgtaaaccac attcaacgat tgatcggaaa
     3601 ccacacggcc gctatcccag agatcgcctt gattgtcacg cagccgttcc ggcgaagatg
     3661 ccaccaagat ctggtaggcg acctggcgca ggtcacgcgc cgaccgacgc gcggcgatca
     3721 gtttccaact cagacgcggg gccgcatggt cgagtcccac agggtcttgc agataatcga
     3781 ccgtcagagc ctgcggggcg gcgagcgcac cgtgctgccc ccacattttt tgtgcgcaac
     3841 ctgcaacaac catcaagccc gccagcatac ataaaaactg tctcattcga ctcccccaac
     3901 ctaaattacc aacacgttga cactgtacac gaccgttcgt gcaggctcaa tcaaaaagga
     3961 aaccattcga atgacgcctc acgttgacac gtgaggttga acgaaacgga aaacttccgc
     4021 gcttcccctc catacgccgg atgtgcgata cttcgcacct gccgaatttc tgttggaatt
     4081 cgtcgatgga aggatcgcgc ggtttcagcg ggttactcga caacttccgc atctacggcc
     4141 ggatcctcaa cgggcaggag ttgcggaacg tgatgctgac ggatgattcg gatgtctcgc
     4201 ggaggctgag catccgcatc cactgaagga ggaccaagtg gaaaactctc ttcccatcgt
     4261 tgcggtgggg ctgagcggcg gcgtggattc agcggtcacg gcttggctgc tccaacagca
     4321 aggccaccgt gtgatcggcc tgaccatgag catctgggat ccgtcgttcg gcgcggccac
     4381 cgcgaaacag agcggctgtt tcggacccgg cgaagcggag aatctcgcgg cggcccgcgc
     4441 gatcgccgaa cgtctcggca tcccccacca caccatgcat ctggcccgcg agtacgggtg
     4501 cggggtcttg gacccgttcc gcgcacagca tctggagggc cgcacgccca atccctgcgc
     4561 cctctgcaat ccgatcatga aattcgggct cctgcccgca cgcgcccggg agaagggaat
     4621 cgggttcgac cgcttcgcca ccggccatta cgcacggatc gtccaagacg gggaaacggg
     4681 gttgttccgt ctgctacgcg gcgtggaccc caccaaagag cagtcctatt ttctcgcgcg
     4741 cctgacccag gcgcaactgg cccgaatcct ctttccgatc ggcgcgatgg ccaagtctga
     4801 ggtgcgcgag accgcccgac gcgcggggct gggtgatctg accgaaaaac cggagagtca
     4861 ggatttcttc gaaggtgagg acatcgccgc gcttttccac ggtgcgggaa ccgagccggg
     4921 accgatcacg gatgaggcgg gccgggtact gggccagcat cgcggcatca tccgctacac
     4981 tgtcggacaa cgggacggcc tcggcatcgc ggtcggtcac aaggtttacg tgaagacgat
     5041 tcatgctgac accaacacgc tggtggttgc cccgcgcgag ggggtctttt ccgacaggtg
     5101 ccgcgtcaac gacttccact ggatcgcggg cgaaccgccg caaccggagc gcgcgctcac
     5161 cgcgcaccta cgttaccggc acaaaggggt ttcggttcaa atcacgttcg ggccccaccc
     5221 cgaaacgttc gacctgcttt tcgcggaacc gcagttcgcc gtgaccccgg gccagatggc
     5281 cgtgctctac gacggtgatg aggtgctggg cggcggttgg atcgcagtgt gatgatccgt
     5341 tcgcaacagg agggaggaga cgtgccgtgg aggcggttgc cgtaatcggt ttccctgccg
     5401 gacttgacga acgcccgtca cctgtgagac aaaacgtgtg ttgggatcct tcgttcagga
     5461 ataagcctca tgacatgtaa agatattccc gccgttaacc ggctgctgaa aaccgccttc
     5521 cacagcatgc gcgccccgat tgtcgatctg atcgccgcgc agacccgcga cccgttccgg
     5581 gtactgctcg gcaccatcct gagcgcacgc acccgagacg cctgcaccgc cgaggcctgc
     5641 aagcgtcttt tcgcgcgggt caccacgccg gacgcgttgc ggcgcattcc ccaagacgaa
     5701 ctcgaacggc tgatctttcc cgtggggttc tatcgcgata aagcgcgcca cctcaaagcc
     5761 ctgccgcaag ttctggaaca acggtttggc ggcgtactgc ccgatacggt cgaagaactc
     5821 tgcgaactgc ccggcgtggg gcggaagacc gcgaatctgg tggtggcgct gggtttcgac
     5881 aagcccgcga tctgcgtgga cgtccacgtc caccgcatca ccaaccggct cggtctcgta
     5941 aacacgaaaa cacccttcga gaccgagatg gcattgcgcg cctgccttcc caaacggtac
     6001 tggaaaacct ggaactccta ccttgtcgcg ttcgggcaga cacgctgcct gccccgtaac
     6061 ccgcgttgtg acggatgtcc gctagcgacg ttctgcccct cttacgccgg aaaaaaaaga
     6121 gacgcccccg gtcttccgaa ggcgtcacgt cagggctgaa cccattcgat cagaacatct
     6181 cgcgcgctgc gcggcgagag ttgttccgga tacgggcgcg tgccgatttg cggcgggcct
     6241 tttcgcacgg tttctcataa taacgattat tgcggagctg cttcaacaag ccctccttgt
     6301 ccataatctt cttcaaccgt ttgagcgcac gctcgacgga ctcgcctttt ttcaatcgga
     6361 gttggatcac gctgttcacc ccctttcaac ccgacctgcg gcgcggttcc ggcgggtttc
     6421 ccctccatga cacagccgct tggttaaaag aagtaaaata acagaatatg ccgcctgccg
     6481 tcaatccgtg accgggaaaa aagcatttgc cgcgttgaca tccgccccgc tttttgcgat
     6541 atttctggaa ctttttgttg cggtagggta ccgaagtggc caaacggggc agactgtaaa
     6601 tctgctggcg gacgccttcc ttggttcgaa tccaagccct accaccattt tgaaaccttg
     6661 gcgtttgccg aggtttttca ttttctcggg ctgaactcct cctttttgcc gccccccatc
     6721 cccccgcttc ccgtctcccg tccctttcta ccctctcatt gcgttcagcc caataagcat
     6781 tggcttttgt tgagaacccc gccgcacctg tttggtgcag gacgcggcgg tgcgcccgac
     6841 gctctttttg aactcttagt cccggcactt tgtcggttgc acttcgtcgc aagcgcaatc
     6901 gactgagttt tggagaccgg tgcgtatgcg ggactaaagc gtccgccaac cggtagggac
     6961 acccgcttct cccgtcagac cttatatgtc gccccgcgac gagcgacctg cggtgtcgga
     7021 aacgcgggcg tccctgtcga tcgtcgcggg tcgcgggtcg acgatcccga ttccgatagc
     7081 gattccgatc ccgaccc
//
LOCUS       DAOSLE010000136         7036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_9730_length_7036_cov_7.806904, whole genome shotgun sequence.
ACCESSION   DAOSLE010000136 DAOSLE010000000
VERSION     DAOSLE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9730_length_7036_cov_7.806904"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PK576_08720"
     CDS             <1..473
                     /locus_tag="PK576_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE61713.1"
                     /translation="NVRAPFSTNLLLGVMSAGCAWATFGKFPFWMVPMAPDHEEMLPL
                     KGGRMPEPESMETSPVSPDRLTDVFMSGTIHDEDQPCHLKILDRAKCEECIKLYGAPC
                     QRFCPAEVYRLEDGHIHVDFSNCLHCKTCQIKDPFQNIVWDFPQGGDGPRYTRM"
     gene            531..1655
                     /locus_tag="PK576_08725"
     CDS             531..1655
                     /locus_tag="PK576_08725"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61714.1"
                     /translation="MNGFLCLICGDDDYLVEAAARERIARFLPEADRALGLEIIDGRR
                     DTGDEARKAAEACMESVQTPGFFGAVKVTWLRNATFLTGGGRVAESLAAKEAVERLTI
                     WLKGGLEEGQVLVITAARILRSSKFFKLCQKQGEVEDFGGNLKSWELEKRASQRLDGL
                     LKKAGLTLDEEAYREFLNRVGWETRLLVQEVEKLRLYIGASGRVTIEDVRTITSVGRE
                     AEAWDLLDAFGERKPAEMLKALSRMDGQKGIGIMLAVMLEKRVRELLVLRAAYDRKWL
                     TTSGWQRDLPPEAVEQLAALPVNDKTMSAWMLKKRMPQVLAYSLQELRGARFRLLDLR
                     EKLVSTGLPERFLIETVLLRIMARRAVSRPAARQRVAGAR"
     gene            1652..4075
                     /locus_tag="PK576_08730"
     CDS             1652..4075
                     /locus_tag="PK576_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61715.1"
                     /translation="MTRRTQRTGWRALAAAGLLGAAGAVSAASLDLNRDGVVRVAVLS
                     APRQAGGMGMDAFVADLESMIRAADASVPARVTLEPVGDGCRTLMGWWYHPDRQSERK
                     RLLTGQYDLLLLAEDESVVLGYPEFFFEGVRALSQAAGGARIRPALVLLAKPATSFRD
                     KRLDAVAELTYRVGDGCGVEVIPAAYAWYETLRRNRMSGNSSVRVRAATFLAAATVYT
                     HLSGDRVPKAAWETGWTTKKITSALVQSATEAVANERVRAHYKGPFRGVVRIDPRITR
                     RLKVYVPNTAEDDPVRQNLQYILDAAFQDGFWRSPSDWYRSGFDRYAAAFDLVYGDRQ
                     QMDLYLDPARYTSLGMGVATNGPQPCAAVYSRTPQGDGVDGRDTLRTLEANLLEGYDH
                     AKANGLTFIPYPLAWARVYRENAELVREVVPGSANDWLVYMLANMLYTVVTDRCQPPP
                     DKRKPGGGNGAHPHGYHEVCARIGYETVVQLANLSQPVNTLLLRSETYRIDRQNPGFA
                     GVRLLNRPSSEVRVFCATDIPGVAQLSRETLVFTPENYDIEQTVRVAPATDRPTQFFH
                     FMASAQSEDRAIDGANDLRPFLLNYDASEEGSVVFDRDHLSPQTGFDAVLRPEQRPCD
                     LVRVRVEQHGQITAEIFFSPDYFSGMPVRIMPSAADYQNGFAQVTVRTLSADERFNNR
                     TFLHTFRVSSGSDPVPQIRVTAPAADSVLDGPAFVAARAEAERSDRVRSVAIYLGPKL
                     LGNSSTPVCAVSVEQAPPQSRLGGGDYTLRAVAIRDDGLVVSSPLLSFRVREPVGQGA
                     PLSNGPPSP"
     gene            4212..5543
                     /locus_tag="PK576_08735"
     CDS             4212..5543
                     /locus_tag="PK576_08735"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOE61716.1"
                     /translation="MRWIVYNLLFAFVYTAMMPVFLFRMKRRGGYRERMGDRFGCYPD
                     GIARQLRDMRGAVWIHAVSVGEVYVAGQVMRALRERIPSLRFVFSTTSSTGWREAEKQ
                     LGTSDVLIYNPLDFPVCVRRAMSRIRPAALILTESEIWPNLIRACHGCGVPLYLVNAR
                     VSDRSAPGYRRLRFWFGPVLRKFTAILAQSEEDRRRLVAAGADPARVEVTGSFKFDVA
                     NRDPEAEKAVAAYLDRWDAGAGRVVLLGGSIWPGEDVVLITLYARLRRIFPALRLVLV
                     PRHFEKADTVEAAIRRAGFACARKSRTPLPEEGTGAPEGGATIVLGDTTGELMGFYGN
                     AALVFVGKSLCEHGAQNMIEPCLCGAATLIGPHTENFRPVVSDLLEAGALIQVPDAEA
                     LEREIARLLADDAARRALGERASAAVLRRKGVVGRCADTLAAELRKTGCVR"
     gene            5579..6604
                     /locus_tag="PK576_08740"
     CDS             5579..6604
                     /locus_tag="PK576_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOE61717.1"
                     /translation="MGKTLSVQGIVELASAYYGSAVLFAAVESDVFTRIAAAGGEAEI
                     GPLAEDLGADPRGLRLLLDACVAIGLLTKTEERYAVTPAAAMTLVAGAPQDLTRAVRY
                     NRDVYPAWGRLTELVRTGRPVEAPECHLGADADRTRRFVLSMHGRALGIGRGVVPLID
                     VSGCRTLLDLAGGPGTYAVLLAQANPGLRCVTVDLPEISAVAAELVDGAGMADRVICR
                     PGDYHTDRYEPEAYDAVTIFGALHQESPERIPDILRRAADALRPGGRIHILDMMTDAT
                     HTWPAFSALFAVNMALTTRDGWVFSDGELHGWLREAGFEACATLPVPPPMPHWLVSAR
                     KPDHGRQ"
     gene            complement(6589..>7036)
                     /locus_tag="PK576_08745"
     CDS             complement(6589..>7036)
                     /locus_tag="PK576_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61718.1"
                     /translation="SARRRLRHRPKEGYTVRMFTPFPSFCRDAAGGFIRLFVLTLLCG
                     CAALRFDVTRKARFINMDSEILHVDYGSEKRSETLPNGLVCTFEGKVRLLLPGDNRRV
                     TLYQAISTTGVRYVSEDKQLEFIEKGPYCIVLRNGQTIFEGVYCRP"
BASE COUNT         1184 a         2043 c         2461 g         1348 t
ORIGIN      
        1 agaacgtgcg cgcgcctttc tcgaccaacc tgttgcttgg cgtgatgagc gcgggttgcg
       61 cctgggcgac cttcggcaag ttccccttct ggatggtgcc gatggcaccc gaccacgagg
      121 agatgctgcc gctcaagggc gggcgcatgc cggagccgga gagcatggag accagtccgg
      181 tctcgcccga ccgcctgacc gacgtcttca tgtccggcac gatccacgac gaggatcagc
      241 cgtgccacct gaagattctg gaccgcgcga agtgcgagga gtgcatcaag ctctacggcg
      301 cgccctgcca gcgcttctgc ccggctgagg tgtaccgctt ggaggacggc cacatccatg
      361 tggacttctc caactgcctg cactgtaaga cctgccagat caaggatccg ttccagaaca
      421 tcgtgtggga ctttccgcag ggcggtgacg gcccccgtta cacacgcatg taaagttgat
      481 acggggcggt ccttctcgcc attgcaccgg ggttccgcta tactcacgac atgaatggtt
      541 ttttgtgtct gatctgcggg gatgacgatt atctcgtcga agcggccgcc cgggagcgga
      601 tcgcgcggtt tctgccggag gcggaccgtg cgctcgggct ggagattatc gacgggcgcc
      661 gcgacaccgg ggacgaggcc cgcaaggcgg cggaagcgtg catggaatcg gtgcagaccc
      721 cgggtttctt cggcgcggtc aaggtaacgt ggctacgcaa cgctacattt ctgactggcg
      781 gcggacgggt tgcggagagt ttggctgcca aggaagcggt tgagcgtctg acaatctggc
      841 tgaagggcgg gttggaagag gggcaggtgc tggtgatcac cgcagcccgg attttgcggt
      901 cctcgaaatt tttcaagctc tgccagaagc agggcgaggt ggaagatttc ggaggaaacc
      961 tcaagtcctg ggagttggag aagcgggctt cgcaacgtct ggacgggctg cttaaaaagg
     1021 ccgggttgac cttggacgaa gaagcgtacc gcgaatttct gaaccgggta ggctgggaga
     1081 cgcggctgct ggtgcaggag gtcgagaaac tgcgtcttta tatcggtgcg tccggccggg
     1141 tgacgattga ggatgtacgg acgatcactt cggtcggccg cgaagctgag gcgtgggatc
     1201 tgctcgatgc gttcggtgag cgcaagccgg cggagatgtt gaaggcactg tcgcggatgg
     1261 acgggcagaa ggggatcgga atcatgctcg ccgtgatgtt ggagaagcgg gtccgcgaat
     1321 tgttggttct gcgtgcggca tacgaccgca agtggctcac cacctcggga tggcagcggg
     1381 atttgccgcc cgaggcggtc gaacaactcg ccgccctgcc ggtgaacgac aagacgatga
     1441 gcgcgtggat gttgaaaaag cggatgccgc aggtgttggc gtattcgcta caggaattgc
     1501 ggggcgcccg gttccggctg ttggatctgc gcgaaaaact cgtctccacg ggtctgcctg
     1561 aacgtttcct gatcgaaacc gtgctgttgc ggattatggc gcgccgcgcc gtttctcggc
     1621 ctgcggcgag gcaacgggtg gcgggagcgc gatgacgcga aggacgcaga gaacgggatg
     1681 gcgcgcgctg gccgcagcgg gactgctggg ggccgccggt gccgtgtcgg cggcctcgct
     1741 ggatctcaac cgggacggag tggtgcgggt ggcggtgctg tcggccccgc gacaggcagg
     1801 cggaatgggt atggatgcgt tcgtcgccga cctcgaaagc atgatccggg cggcggatgc
     1861 cagtgtgcct gcgcgtgtga ccctcgagcc cgtcggtgac gggtgccgca cattgatggg
     1921 atggtggtac caccccgacc gccaaagcga gcgcaagcgt ctgttgaccg gacaatacga
     1981 tttgctgttg ctggcagagg acgagtcggt ggtgctggga taccccgaat tttttttcga
     2041 gggggtgcgc gctttgtcgc aggcggcagg gggtgcgcgg atccggccgg cgttggtgct
     2101 gctcgccaaa ccggccacct cgtttcggga caagcgcctg gacgcggtgg ccgagttgac
     2161 ctatcgtgtg ggcgacggct gtggcgtgga ggtgattccg gcggcatacg cctggtacga
     2221 gacgttgcgg cgcaaccgca tgtccggcaa ctcttcggtc cgggtacggg ccgcgacctt
     2281 tcttgccgcc gccaccgtct atacgcacct gagtggcgat cgtgtgccca aagccgcatg
     2341 ggagaccggt tggacaacca aaaaaatcac gtccgcgctg gtgcagtccg cgacagaggc
     2401 ggttgccaat gaacgggtcc gcgctcacta caaaggcccg ttccgcgggg tggtgcggat
     2461 cgatccgcgc atcacccgac gtctcaaggt gtatgtgccc aacacggccg aggatgatcc
     2521 ggtccgccag aatctgcaat acattttgga tgccgcgttt caggacgggt tctggcggtc
     2581 gccttccgac tggtaccgtt ccggattcga ccggtatgcc gcggcgttcg atctcgttta
     2641 cggcgaccgg cagcagatgg atctgtatct ggatcccgcg cggtacacct cgttgggcat
     2701 gggtgttgcc accaacgggc cccagccctg cgcggcggtc tactcccgca cgccgcaggg
     2761 cgatggggtg gacgggcgtg acacgctccg gaccttggag gcgaacctgt tggaggggta
     2821 tgatcacgcc aaggccaacg gcctgacctt tatcccgtat ccgctcgcat gggcgcgcgt
     2881 gtaccgtgag aatgccgaac tcgtgcggga ggtcgtgccg ggaagcgcca atgactggct
     2941 cgtctacatg ctcgcgaaca tgctctacac tgtggtcacc gaccgctgcc agccgccccc
     3001 cgacaagcgc aaaccaggag gggggaacgg agcccatcca cacggctatc acgaggtgtg
     3061 cgcccgtatc gggtacgaga cggtcgtgca gttggcaaac ctgtctcaac ccgtgaacac
     3121 cttgcttttg cgcagcgaga cctaccggat cgaccgccag aaccccggtt tcgcaggggt
     3181 ccgattgctc aaccgccctt cgagcgaggt ccgtgtcttt tgcgccaccg acattcccgg
     3241 cgtcgcccag ttgtcgcgtg aaactttggt gttcaccccg gaaaactacg acatcgagca
     3301 gaccgtacgg gtcgcgcctg cgaccgaccg tcccacgcag ttctttcatt tcatggccag
     3361 tgcgcaatcc gaagaccggg cgatcgacgg cgcgaacgat ctgcggccct ttctgctgaa
     3421 ttacgatgcg tcggaggagg ggagtgtcgt gtttgatcgc gatcaccttt cgccccagac
     3481 cggattcgat gcggtgttgc ggcctgagca acggccttgt gatctggtgc gcgtccgtgt
     3541 ggagcagcac ggccagatta cggcggagat ctttttcagt cccgattatt tctcgggcat
     3601 gccggttcgg atcatgccct cggccgcgga ttaccaaaac gggttcgcgc aggtcacggt
     3661 acgtaccctt tctgcggacg aacggttcaa taaccgcacc ttcctccaca ctttccgcgt
     3721 ttcaagcggc agtgatcccg tgccgcagat ccgcgtcacc gcgcctgcgg cggacagtgt
     3781 tctggacgga ccggcttttg tggcggcccg tgccgaagcg gaacggtccg acagggtgag
     3841 gtctgtggcg atctatctcg gacctaaact gctggggaat tcgtccacac cggtctgcgc
     3901 ggtgtcggtg gagcaggccc cgccccagtc ccgtcttggc gggggcgact acacgttgcg
     3961 cgccgtggcg atccgcgacg acgggctggt ggtttcttcc ccgctgctct cgtttcgtgt
     4021 gcgcgaaccc gttggtcagg gcgcccccct ctcgaacggc ccgccgtccc cctgaccttt
     4081 ggcgtcggaa tttcggatga ccgcgttccg cgtcttgaca ggctgaacac tgcgcaccta
     4141 cacttatacc tttcaaaacg ggtcgggtgc ggtatgccgc gcacggtacg gcggagggat
     4201 gggaccggtc catgaggtgg atagtttaca acctgttgtt tgctttcgtg tatacggcga
     4261 tgatgccggt cttcctcttc cggatgaagc ggcgcggcgg gtaccgcgag cgtatgggcg
     4321 accggttcgg atgttatcct gacggcatcg cgcggcaact ccgcgacatg cgcggggcgg
     4381 tgtggatcca tgccgtgagc gtgggtgagg tgtatgtggc cgggcaagtg atgcgggcgc
     4441 tgcgggagcg gatcccttcg ctgcgcttcg tgttcagcac caccagttcc accggatggc
     4501 gtgaggcgga gaaacaactc ggcacgtcgg atgtgctgat ctacaacccg ctggattttc
     4561 ccgtgtgcgt ccggcgggcg atgagtcgga tacggcctgc ggccctgatc ctgacggaat
     4621 cggagatttg gcccaacctg atccgtgcct gccacggttg cggcgtgccg ctttatctgg
     4681 tcaacgcgcg cgtatcggat cgttcggcac cgggataccg acgcctgcgt ttctggttcg
     4741 ggccggtgtt gcgcaagttc accgcgattc tggcccagtc ggaagaggac cggcggcggc
     4801 tggtcgccgc aggcgctgac cctgcgcgcg tcgaagtcac cggcagtttc aaattcgatg
     4861 tggcgaaccg cgatcctgaa gcggaaaagg ctgtcgccgc atatctggat cgttgggatg
     4921 cgggggccgg gcgtgtcgtg ttgctgggcg ggtcgatctg gccgggcgag gatgtcgtgc
     4981 tgatcacact ctacgccagg ctgcggcgca tctttccggc attgcggctg gtgctggtgc
     5041 cgcggcactt tgaaaaggcg gacacggtag aagccgcgat ccggcgggcc gggttcgctt
     5101 gtgcgcggaa gagccggacc ccgttaccgg aggaggggac gggggcaccg gagggtggcg
     5161 cgacgattgt gctgggcgac accaccggcg aactgatggg cttctatggg aatgcggcgc
     5221 tggtgtttgt cggcaaaagc ctctgtgaac acggcgcgca aaacatgatc gagccctgtc
     5281 tctgcggcgc cgccaccctg atcggaccgc atacggaaaa tttccgtccg gtggtgtccg
     5341 atctgctgga ggccggggca ctgatccaag tgccggacgc cgaggcgttg gaacgcgaga
     5401 tcgcccggtt gctggcggat gacgcggcgc ggcgcgcgct cggggaacgc gcgtctgcgg
     5461 cggttctgcg ccgcaagggg gtggtcgggc ggtgtgccga cacactggct gcggagttgc
     5521 ggaaaaccgg atgcgtccgg taggggcccg gagcaaagga aagaacggga gagacgggat
     5581 gggcaagacg ctgtcggtgc aggggattgt tgaattggcc agcgcgtatt acggctcggc
     5641 ggtattgttc gcggcggttg agtccgatgt gttcacgcgg atcgctgcag cgggcgggga
     5701 ggcggagatc ggcccgttgg cggaggatct gggagcggac ccgcgcggtc tgcgtttgtt
     5761 gctggatgcc tgtgtggcca tcggcctgct gacgaagacc gaggagcggt acgctgtgac
     5821 accggccgcc gcgatgacgc tggtggcggg cgcaccgcag gatctcacgc gtgcggtacg
     5881 gtacaaccgc gacgtgtatc cggcatgggg tcggctcacg gagttggtcc ggaccggcag
     5941 gccggttgag gccccggagt gtcatctcgg cgcggacgcc gaccgcaccc gacgtttcgt
     6001 gctctcgatg catggccgag cactggggat cgggcgtggc gtggtgccgc tgatcgacgt
     6061 gagcggatgc cgtaccctgt tggatctggc gggcggtccg ggcacctatg cggtgctgct
     6121 ggcacaggcg aatcccgggc tgcggtgtgt gacggtcgat ctgccggaga tctccgctgt
     6181 ggccgctgaa ttggtggacg gggccgggat ggcggatcgc gtcatctgcc gccccggcga
     6241 ttaccatacg gaccgatatg agccggaggc ctacgacgcg gtgacgattt tcggcgccct
     6301 gcatcaagaa tcaccggaac ggatcccgga tattctgcgg cgcgcggccg acgccttgcg
     6361 ccccggcgga cgaatccata ttctggacat gatgaccgac gcaactcaca cgtggccggc
     6421 tttctccgcg ctgtttgcgg tgaacatggc gctcaccacg cgcgacggat gggtcttctc
     6481 ggacggggag ttgcacggtt ggttgcggga ggcgggattc gaagcgtgtg cgacgctccc
     6541 cgtgccgcca ccgatgccgc actggctggt cagtgcgcgt aagccggatc acggtcggca
     6601 gtagacccct tcgaagatcg tctggccgtt tcggagcacg atgcaatagg ggcctttttc
     6661 gatgaactcc agctgcttat cctctgagac ataccgcaca cccgtggtgg agatcgcctg
     6721 atagagcgtc acccgcctat tgtctccggg gaggaggagg cgcaccttgc cttcaaaagt
     6781 gcagaccagt ccattgggca gggtttcgct gcgcttttcc gaaccgtagt caacatgcag
     6841 gatctcggaa tccatgttga tgaagcgcgc cttgcgcgtc acgtcaaaac gcaacgcggc
     6901 acagccgcac agcagcgtca gcacaaacaa ccgaatgaaa ccacccgccg catcacggca
     6961 gaaagatggg aatggggtga acatccgtac agtatacccc tccttgggcc ggtgccgcaa
     7021 gcgtcgccgt gccgat
//
LOCUS       DAOSLE010000137         6827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10114_length_6827_cov_7.559657, whole genome shotgun sequence.
ACCESSION   DAOSLE010000137 DAOSLE010000000
VERSION     DAOSLE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10114_length_6827_cov_7.559657"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="PK576_08750"
     CDS             complement(<1..1268)
                     /locus_tag="PK576_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE61719.1"
                     /translation="MTKKTILTVVTSVTILTASAFDALKDRPTPPRQIFPEISAAQRD
                     ARRVWWTQSRFGMFIHFGLYASPARHEWVQSRERIAPDVYAKRYLPQFNPDLFDARDW
                     ARRAKQAGMKYMVLTAKHHEGFCMWDTKTTDYKITNTAFKRDLVREYVEAARAEGLRV
                     GFYFSIKDWHHPDYLIDATHPLRPADGRFTDEIYEKLNAGRDWNRYRTYMYDQVKELL
                     SDYGKIDIIWYDYTVKTKYGKNWKHWESVELLKLTRTLQPDILVDSRLDLMDTEDGWD
                     FVTPEQCRVTQWPQVDGKKAPWETCQTFSGSWGYYRDEETWKNPHQLIELLINSVSFG
                     GNLILNVGPTARGEFDYRACERLDAIGAWMRVNSRAIYDCTAAPDAFKKPVDSLLTYN
                     PETNRLYIHLVNYPMGYLPFDFADKVEYAQ"
     gene            complement(1322..2347)
                     /locus_tag="PK576_08755"
     CDS             complement(1322..2347)
                     /locus_tag="PK576_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61720.1"
                     /translation="MTKDFPEVTSAEIKALAKRVGADCVGIAPMSRWEGVPKQNDPRY
                     IFPDAKSMIVLGFRIPRGLLRGIEEGTRFIDYPGMGYASINQVHGPMVLWKLNAYLEE
                     RGYEVFPLQNANGGDAVNPVTGKFREGLSRHVAEGLPLPDVLVHFRVAAYLAGLGEFG
                     WSKMFLTPEFGPRVRFAMLFTDAEFDTYDPIYAGKICDRCKLCVKHCGGNAISMNESV
                     KVTLAGRVVEWGKLDELACEEGLHGGEGRELSPFDGEYPRQYGYGRAVEGACGCIRAC
                     MVHLEQRNKLTKTFRNPFRKPDWKQWKVDHTKPYEIPPEVVAEYGLDKPQAKEESDAE
                     AFNPLND"
     gene            complement(2349..3422)
                     /locus_tag="PK576_08760"
     CDS             complement(2349..3422)
                     /locus_tag="PK576_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61721.1"
                     /translation="MAYEGELTAADVKAKAKECGADIVGIAPMSRWEGTDKQHDPRYI
                     FPDAKSMIVFGFRIPRGSLRGIEEGTHFLNYATMGYAALNSIYGPMVLWEMNRYLEDH
                     GYEAVPMANVSGGEAVNPLTGNFREGWSRPVKPGLPAPDVSVDFRVAAFLAGLGEFGY
                     SRAFLTPEFGPRQRFAIMLTDADLEPDPIYTGRICDRCMACVKHCYGRCISPTETVRK
                     VIGGVQVEWGKRDELACSRAAREGGLNRELSPFDGDYPRKYGYGLDHEGASGCIRACM
                     VHLEERAKMTRGFRRPFRKPGWKQWEIDHSKPYELPPEVRAEYAGNIESHDAYTNYNI
                     TSNYGTKAAAGLVQDDSSSGGVA"
     gene            complement(3428..5206)
                     /locus_tag="PK576_08765"
     CDS             complement(3428..5206)
                     /locus_tag="PK576_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008866738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HOE61722.1"
                     /translation="MGYTVRFNFLDWMAIVLFFAALVGIAFGCSRKASKDAKDFFLSG
                     RSMPWWLIGVSMCAATTSTNSANMFTEFIRNSGLSENWKWWAFLLTGMLTVFVYSKLW
                     VRSGAKSDIEFYELRYSGRAAAFLRGFRALYLGIVFNVITTGLVMLAAIKIGQVLFGL
                     DQWTIIIVTSVCSIIYSALGGFRGAIYTDFLLFILIMIGAVVGMVYALGRPEVGGFAA
                     MMQNPVVQKHLSFLPDVNNTELFVSVFVIPIAIQWWNVWYPGSEPGGGGYIVQRMLSA
                     KSENHCVAGSILYQVINYAIRPWPWYLTAFASLIVFPDLASLQKAFPNVNPALVKGDL
                     AYPAMLTFVPNGWLGVVAASMMGALFSTVAAHLSMGSNYIANDFWKRFVRPDAGEREL
                     IIVGRVTVIVLMVLSGLLAPVLSSAGAVFNLILQIGAGTGLIYLLRWFWMRINAWSEI
                     TAMAVSFIVAVFLQLVYPHLGLPPLLSWHRLLIVMAITTVAWVGVTLVTPPTDAVKRA
                     SFQNKIRANKHDIAWGLLAMTIASVCVYALMFASGCWIYGKTVLAATMTVISIVFGLV
                     LIPVVRKLNAEQSDENCEFQTLNPGV"
     gene            5280..6452
                     /locus_tag="PK576_08770"
     CDS             5280..6452
                     /locus_tag="PK576_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE61723.1"
                     /translation="MKAKVLLILPSDKFLHRQIIEGVLAHGREHGPWQFHFETGDRYE
                     QALGKGRNQACSGIIALVRERSQLAELLTLNKPIVLINPPSPLGGRQKAKPPPHAVYV
                     NRNQEDVGKTAAEYFLERGYRQFAFVGTPQAALWCERRLNGFCKRLADAGFSCNVYTS
                     KINTAADDFSLEAKALSSWLKKLHPGTALYVVRDRRALQVLEICSDIGISVPETISVL
                     STDNDVVLCESTSPSLSSIFLNGENCGQLCARLLDRLLRKRKVEPFVDLAFPQVITRQ
                     STDNTLIPDPFLAKALAIIRKDLSVPHTIEEIAQTLNVSRRTLELKARLVLGKTLKEE
                     IDSIRLNEAVRLLANSSEAIVKIASKCGFCGASHLNTRLKTVFGYGSSVFRYRDPR"
     gene            complement(6658..>6827)
                     /locus_tag="PK576_08775"
     CDS             complement(6658..>6827)
                     /locus_tag="PK576_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HOE61724.1"
                     /translation="AGATIIDSLPHGSFFHATGGAVGMSVKDRLKLIPYEAAVGLAST
                     VVAVVLAACAR"
BASE COUNT         1495 a         2125 c         1868 g         1339 t
ORIGIN      
        1 aactgcgcgt attcgacttt gtccgcgaag tcgaacggca ggtagcccat cggatagttg
       61 acgaggtgga tgtagagccg attcgtttcg ggattatagg tgagcagcga atcaaccggc
      121 ttcttgaacg catccggcgc agccgtgcaa tcatagatcg cgcggctgtt cacacgcatc
      181 cacgcgccga ttgcatcaag acgctcgcat gcgcgataat caaactcgcc gcgggcggtc
      241 ggaccaacgt tgaggatgag attgccgccg aacgaaaccg agttgatcag aagttcgatc
      301 agttggtgcg gattcttcca ggtctcttca tcgcggtagt agccccacga accggagaac
      361 gtctgacaag tctcccacgg tgccttcttt ccatccacct gcggccactg cgtgacacgg
      421 cactgctcag gcgtgacgaa gtcccatccg tcctcggtgt ccataagatc aaggcggctg
      481 tcgacaagga tgtcgggctg gagggtgcgc gtcagcttca gaagttctac cgactcccag
      541 tgcttccagt tcttgccgta ttttgtcttt accgtgtagt cataccagat gatatcgatc
      601 ttgccatagt cagaaaggag ttccttcacc tgatcgtaca tgtaggtgcg gtagcggttc
      661 cagtcccgcc cggcgttcag tttctcgtag atttcgtccg tgaacctgcc gtccgcaggc
      721 cggagcggat gtgtcgcatc gataagatag tcagggtggt gccaatcctt gatcgagaag
      781 tagaaaccga cgcgaagccc ttccgcacgc gccgcctcga catactcgcg cacgagatca
      841 cgcttgaacg cagtgttggt gatcttgtaa tcggtcgtct tcgtgtccca catgcagaat
      901 ccttcatgat gcttcgccgt caggaccatg tatttcatgc cagcttgctt cgcgcgtcgc
      961 gcccaatccc gcgcatcgaa gagatcggga ttgaattgcg gcagatatcg cttcgcataa
     1021 acgtcgggcg cgatccgttc cctcgactga acccactcat gccgcgccgg cgacgcatag
     1081 agaccgaagt gaatgaacat gccgaagcgg ctttgcgtcc accaaacccg tcgggcatct
     1141 cgttgcgcgg cggaaatctc ggggaagatc tgccgcggag gcgtaggccg atcctttagc
     1201 gcgtcaaagg ctgaagccgt cagaatcgtc acagatgtca caaccgttag aatcgttttc
     1261 tttgtcatgt tttttccttt ctagttcgat gcaacttctt ctcgttatgc ccgtgttatc
     1321 ctcagtcgtt cagcgggtta aaggcttcgg cgtcggactc ctctttggct tggggtttgt
     1381 cgagaccgta ttccgccacg acttcgggcg ggatctcata gggctttgtg tgatcgacct
     1441 tccactgttt ccaatccggc ttacggaacg gattgcggaa ggtcttcgtc agtttgttgc
     1501 gctgctcgag atgcaccatg cacgcgcgga tgcagccgca agcgccttcg acagcacggc
     1561 cgtaaccgta ctgccgcggg tactcgccgt caaacggcga cagttcgcgt ccctcaccgc
     1621 cgtggagccc ttcctcgcaa gcgagttcgt caagcttgcc ccactcgacc acgcgtccag
     1681 ccaaggtcac cttcacggac tcgttcatcg agatcgcgtt cccgccacag tgcttcacac
     1741 agagcttgca gcggtcgcag attttgcccg cgtagatcgg gtcgtatgtg tcgaactcag
     1801 catccgtgaa aagcatcgcg aaacggacgc gcgggccgaa ctcgggcgtc aggaacatct
     1861 tcgaccagcc gaactcgccg agacccgcga gatacgccgc gacgcggaag tggacgagga
     1921 cgtccggcag gggaagccct tccgcgacat gacggctcag accctcacgg aacttgcccg
     1981 tgacaggatt caccgcatca ccgccgttcg cgttctgaag cggaaagacc tcatagccgc
     2041 gctcttcgag gtaggcattc aacttccaga gcaccatcgg gccgtgtacc tggttaatcg
     2101 aagcgtaacc catgccggga tagtcgatga agcgtgtccc ttcctcgatg ccgcggagaa
     2161 gcccgcgcgg aatacggaag cccagaacga tcatcgactt cgcgtccggg aagatgtaac
     2221 gcgggtcgtt ctgcttcggc acaccctccc agcggctcat cggcgcgatg ccgacacagt
     2281 ccgcacccac gcgcttggcg agcgccttga tctctgcgga cgtgacctct ggaaaatcct
     2341 ttgtcatatc aggcgactcc tccgctggac gaatcatcct gcacgagtcc ggccgcagcc
     2401 ttcgtaccgt agttggatgt gatgttgtag ttcgtatagg cgtcgtgcga ctcgatgtta
     2461 cccgcatatt ccgccctcac ttctggcggc agttcgtagg gcttcgaatg atcgatctcc
     2521 cactgcttcc agcccggctt acggaacggc cggcggaaac cgcgcgtcat cttcgcccgc
     2581 tcttcgagat gcaccatgca cgcccggatg caaccgctgg caccctcgtg atcaagcccg
     2641 tagccgtact tgcgcggata gtcgccgtcg aacggcgaaa gctcgcggtt gagtccgccc
     2701 tcgcgagccg cccgcgaaca tgcgagttcg tcccgcttcc cccactcgac ctgtacgccg
     2761 ccgatgacct tccgcaccgt ctcggtcggc gagatgcaac gcccgtagca gtgcttgacg
     2821 cacgccatgc agcggtcgca gatacggccc gtatagatcg gatcgggttc gagatccgcg
     2881 tcggtgagca tgatcgcgaa gcgttgccga gggccgaact cgggcgtcag gaacgcgcgc
     2941 gaatagccga actcgccgag acccgcaagg aacgcggcaa cccggaagtc gacggaaacg
     3001 tccggcgcgg gaagcccggg cttcacggga cggctccagc cctcgcggaa gttgcccgtg
     3061 agcgggttga ccgcttcgcc gccggagacg ttcgccatcg gcacagcctc gtagccatga
     3121 tcttccagat agcggttcat ctcccacagc accatcggcc cgtagatcga gttgagcgcg
     3181 gcatatccca tcgtcgcgta gttgaggaag tgcgtcccct cctcgatgcc gcgcaacgat
     3241 ccgcgcggga tgcggaaacc gaagacgatc atcgacttcg cgtccggaaa gatgtaacgc
     3301 ggatcgtgct gcttgtccgt tccctcccac cgggacatcg gcgcgatgcc gacgatatca
     3361 gccccgcact ccttcgcctt ggccttcaca tcggctgcag tcagttctcc ttcgtatgcc
     3421 ataaccgtta aactcccgga ttcaaggttt gaaactcaca attttcgtct gattgttccg
     3481 cattcagctt cctgacgacg gggatgagga caagaccgaa aacgatggaa atgaccgtca
     3541 tcgtcgcggc gagtaccgtc ttgccgtaaa tccagcagcc cgaggcaaac atcagcgcat
     3601 agacgcagac gctcgcgatc gtcattgcaa ggagccccca cgcgatgtcg tgtttgttcg
     3661 cacggatctt gttctggaat gacgcgcgct tcaccgcatc cgtcggcggc gtcacgagcg
     3721 tcacgcccac ccacgcgacc gtcgttatcg ccatcacgat caggagccgg tgccaagaaa
     3781 ggagcggcgg caggcccaga tgcgggtaaa cgagctgcag gaacaccgcg acaatgaacg
     3841 aaaccgccat tgcggtgatc tcgctccaag cgttgatgcg catccagaac cagcgcagga
     3901 gatagatgag ccccgtgccc gcgccgatct ggaggatcaa gttgaagacc gcgccggcgg
     3961 acgaaagaac cggcgcaaga agccccgaga gcaccatcag cacgatgacc gtgacgcgtc
     4021 cgacgataat aagctcgcgc tcacccgcgt cgggacgaac gaagcgcttc cagaaatcat
     4081 tcgcgatata gttcgacccc atcgagagat gcgccgcgac cgtcgagaac agcgcgccca
     4141 tcatcgatgc ggcgacgacg ccgagccagc cgttcgggac gaaagtgagc atcgccggat
     4201 aggcgaggtc acccttcacc agcgcggggt tcacgttcgg gaacgccttc tgcagggagg
     4261 cgagatccgg aaagacgatc agcgaggcaa acgccgtcag gtaccagggc cacgggcgaa
     4321 tcgcgtagtt gatgacctga tagaggatcg agccagcaac acaatggttc tcgctcttcg
     4381 ccgagagcat gcgctggacg atgtagccgc cgccgcccgg ctcgctgccc ggataccaga
     4441 cgttccacca ctggatcgcg atcgggatca cgaacacaga aacaaagagc tccgtgttat
     4501 tcacgtccgg cagaaacgag agatgcttct gaacaaccgg gttctgcatc atcgccgcga
     4561 acccgccgac ctcgggacga ccgagggcat acaccatgcc caccaccgca ccgatcatga
     4621 tcaggataaa gagaaggaag tccgtataga tcgcaccgcg gaacccccca agcgccgaat
     4681 agatgatcga gcagaccgac gtcacgatga tgatcgtcca ctggtcgagc ccgaagagca
     4741 cctggccgat cttgatcgcc gcgagcatca cgagccccgt ggtgatcacg ttgaacacga
     4801 tcccgaggta cagcgcgcgg aaaccccgca ggaaggccgc agcccgcccc gagtagcgga
     4861 gctcgtagaa ctcgatatcg ctcttggcgc cggaccgcac ccagagcttc gagtagacga
     4921 acaccgtcag catcccggtc aggaggaacg cccaccactt ccagttctcg gaaagacccg
     4981 agttgcggat aaactccgtg aacatgttgg cggaattcgt agacgtggtt gcggcgcaca
     5041 tggatacgcc gatcaaccac catggcatcg accgcccgga aaggaagaaa tccttcgcgt
     5101 ccttcgatgc tttgcgcgaa cagccaaacg cgataccaac aagcgccgcg aaaaacagga
     5161 cgatcgccat ccagtcaaga aaattgaatc tgacggtgta tcccatttga tagaattgta
     5221 gcgaaaacgc attgccctta atagacataa acgcgaaaaa tatatacaat aaatgcgaaa
     5281 tgaaagcgaa ggttctgctc atcctgccgt cggataagtt ccttcaccgg caaattatcg
     5341 agggcgttct cgcgcacggg cgcgagcatg gcccttggca gtttcacttc gagaccgggg
     5401 accgttacga gcaggcgctc ggcaagggtc gcaatcaggc ttgctccggc atcatcgcct
     5461 tggtgcgtga acgaagccaa ctcgcagaac tgcttacgct gaacaaacct atcgttctca
     5521 tcaatccgcc ctccccgctc ggaggacgtc aaaaggcaaa accaccaccg catgccgttt
     5581 atgtcaaccg gaatcaggag gatgtcggca aaaccgctgc ggagtatttt ctggaacgcg
     5641 gataccgaca gttcgccttt gtcggaacac ctcaagccgc actctggtgc gagcgccgtt
     5701 taaacggatt ttgcaagcgt ctggctgatg ccggcttttc ctgcaacgtc tacacctcca
     5761 agatcaatac tgcggcagat gacttctctc tggaagccaa ggcgctctca tcgtggttaa
     5821 agaaactgca tccgggaacc gccctttacg tcgtacgtga ccggcgagca cttcaggttc
     5881 tggagatctg ctccgatatt ggcatctccg tgcctgaaac gatatcggtt ctcagcacag
     5941 acaatgatgt ggttctctgc gaatcgacaa gcccctcgct ctcttccatc tttctgaacg
     6001 gcgaaaactg tggacagctc tgcgcacgac ttctggatcg gcttctccgc aaacgcaagg
     6061 tcgaaccctt tgtcgacctt gcgttcccgc aggtcataac ccgccaatcg actgacaaca
     6121 cgctaattcc agaccctttc ctcgcaaaag cactggcaat catccgaaaa gacctatccg
     6181 ttccgcacac catcgaagaa atcgctcaaa cgttgaatgt ctctaggcgt actctggaat
     6241 tgaaggcccg gctagtcctc ggaaagacgc tgaaagagga aatcgacagc atccgactta
     6301 acgaagccgt cagacttctc gccaactcat ccgaagcgat cgtgaaaatc gcctccaagt
     6361 gcggtttctg cggcgcgagc catctcaaca cacgccttaa gacggtcttt ggctacggtt
     6421 cgtctgtttt ccgctaccga gacccacgat aaggtagcca agtcgcagag gttctcgatc
     6481 atattgtgtc ctgtccccta gtcgcaacac ccttatcccc agtctccgat atcccgcaac
     6541 accatctcct catcccgtgc gatgctctgc gcgttcatgc aataggccct atcccgtatc
     6601 gcgccattat ttggatacac tagcgtccca cggaactcac ctacattttg tcacacctta
     6661 ccgcgcacac gcggccagaa cgaccgcgac gaccgtagag gcaagcccga ccgcagcttc
     6721 gtaggggatc aatttcagac ggtcctttac cgacatcccg accgcaccgc cggtcgcatg
     6781 gaagaaactg ccgtgcggca gggaatcgat gatcgtcgcg ccggcat
//
LOCUS       DAOSLE010000138         6782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10190_length_6782_cov_8.852981, whole genome shotgun sequence.
ACCESSION   DAOSLE010000138 DAOSLE010000000
VERSION     DAOSLE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10190_length_6782_cov_8.852981"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..3036
                     /locus_tag="PK576_08780"
     CDS             400..3036
                     /locus_tag="PK576_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE61725.1"
                     /translation="MEGVREAPILTDPVSAAACPKCGAMLDVGGLPTFTAVQCPNCET
                     EFQVPAQFGNFLLLQLLGAGGMGGVYRARDEGLNREVAIKVMLKSLGDDPMFVETFQR
                     EAQAAAKLNHPNIAQIYSFGQEKGQPYIVMELVSGGSLDKMMAAQGAMDPTAVIQIGE
                     QIAEGLREAADAGLVHGDVKPENILFDNDKNAKLVDFGLAAMNAGPGNEVWGTPYYIA
                     PEKVRRQKSDFRSDIYSLGATLYHAITGVPPFDGEDATAVVKARFEGPPKPMSELRKN
                     IPDEIEALIRRMLEVEPQMRYPTYGSLLGDMRRYLSKVKPVNLALSSKKIMIKGKLSK
                     TTTGVPTITGNMSDDLPPGMVPVDAIPEEDLESEEEAKRRGHRMMLMILGGVFLGVAM
                     LVGSVFGVKHYLGVKREKTVRAINESRQGKALASIAKSVAQARTLPERLSGYPQEAME
                     TAQAAADAVVAVLGETVRTSMTVPEPTFAWDAQESADAQPPSAPGETAAAPAVSNAVP
                     QGGAAEVTNTVAAAGTNLVTEAGTNASVTAAAPAAPAAPAPEAVPAAGSDDPTAPREG
                     DAHPVVAIVRGMFAEAYAVKGGVLLAEHLVKKIEAEAKAAEGMTDLEQTEALVKHANA
                     LVEKVNGLAYVKEVADVPRKVSQLKKTLESVKTDTASLAEMKRREAQEQEKRRKEEEI
                     AEKKRQEKEAHQKKVEEEKAKVADAELANRDMLKALQFREAVNAIRELRGGLETDEGG
                     IAASIAIDRINRMRDFHNYLIAQVKGFKSARGWSVDAADAKTLSVGGIKIPWADVYNK
                     RPEIIAELINGLVRSEQATKTLKLSEKTRLMTSAALCLNLFYGEMASAQELAKQIALA
                     AAQQFEIEADTIKQLLPGFFTE"
     gene            3038..4420
                     /gene="thrC"
                     /locus_tag="PK576_08785"
     CDS             3038..4420
                     /gene="thrC"
                     /locus_tag="PK576_08785"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOE61726.1"
                     /translation="MRYISTRGGGEPLTFRQTLLTGLARDGGLFLPETFPDVRDRLNA
                     WRTLPYPELAFQIIRLFADDIPEDALRRIVEQSYAGFRHHEVCPVVPVGPVRMLELFH
                     GPTLAFKDVALQFLGNLFEYALAETGAEMNIVAATSGDTGSAAIYGVRGRERIRIFVM
                     HPAGRVSPVQERQMTTVLDANVHNLAVEGTFDDCQNMVKSLFGDLAFRDTFRLGAVNS
                     INWARVLAQIVYYFYAAFRTQEATGAAAVRFSVPTGNFGDIFAGYVAYRMGLPVDQLV
                     LATNENDILARYFNTGVYSLGEVAPTLSPSMDIQVASNFERYLYCLAGGDAAMLRGWM
                     GAFKAEGTLRPDLPPDPVIVAGRGDTEMTLTTIRAFWETHGYLLDPHTAVGVAVGMRF
                     MHPEIPLICLATAHPAKFGDAILRATGQDLAHHPILDALATLPTRVRTVPADVDAVAK
                     IVAATVRGDG"
     gene            4417..4752
                     /locus_tag="PK576_08790"
     CDS             4417..4752
                     /locus_tag="PK576_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOE61727.1"
                     /translation="MIDPATFRCRQCGACCRVPGYVALAEGETEAIAAFLGMDVYAFT
                     SRYTTLTFNRGTLSLTEREDGRCVFLEDDNTCLIQPVKPAQCKGFPFLWSTPALEHVC
                     AAIRPARDS"
     gene            complement(4820..6472)
                     /locus_tag="PK576_08795"
     CDS             complement(4820..6472)
                     /locus_tag="PK576_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE61728.1"
                     /translation="MRKRFLCGSILLLFASQLIGQETAAEGKVWDLVVYGGTSGGIAA
                     AVQARRMALDVVVIEPTQRIGGLTTGGLGQTDIGNKVAVGGIAREFYRAVRAWYAQPA
                     AWIWQKREQYRDGGQTRTDQNEDAMWTFEPSVALKIYEAWVRTNAIPVVRGERLDRAS
                     GVALTRTVPWRILSIRMESGRVFRGKMFIDTTYEGDLMAAAKVSYTVGRESNAQYGED
                     YNGVQAKIDLKYKHHNFLPGVDPYVEKGNAASGLLPFIDPTGPGKEGAGDKRVQAYCY
                     RMCLTDHPENRIPFVKPEGYDPLWFELLLRNFEAGRQGIWINSDMPNRKTDTNNYGGF
                     STDFIGQNYDYPEASYAEREKIAARHLLYQQGLMWTLANHPRVPEHVRKEVSRWGMCR
                     DEFTEGGGWQEQLYVREARRMVSDLVMTQHHCQGKVRAEAPVGLAAYGMDSHNVQRYI
                     DAQGYVQNEGDVELGWLKPYPVGYFAIVPKRKECANLLVPVCVSATHIAFGSIRMEPV
                     FMILGQSAATAAALAIQDDVAVQEVDLAKLQTRLLADGQRLK"
BASE COUNT         1394 a         2033 c         2118 g         1237 t
ORIGIN      
        1 gcggcgggcc aactcctaac tcctaactcc taactcctaa atcctaaata aatcctacat
       61 ccgcgcgtag cgcggaagac gcccgcgttt ccgacaccgc aggtcgctcg tcgcgggtcg
      121 cgggtcgaca taaggtctga cgggagaagc gggcgtccct accccgcgtg gcagggcggt
      181 ttccccgaag ccgaggacga agccgagaaa gcgatcccga taaaaccaga aatcaaagcc
      241 tcagagatcc gtgactcggg gatacgttca gcgtgctggg tttgaaggtt tttcggcatc
      301 gacaacgccc tcttctttcg gcaaactaga atgctttccg gttgacatgg taaaaaacag
      361 gaatggcaca cggatgagcg aacaagcgag ggtggacaga tggaaggtgt gagagaagct
      421 ccgattttga cggatccggt gagtgcggcg gcatgtccga aatgcggcgc gatgctggat
      481 gtcgggggat taccgacgtt taccgcagtt cagtgcccga actgcgagac ggaattccag
      541 gtgcctgcgc agttcggtaa tttcctgctc ctgcaactgc tgggcgcggg cggcatgggc
      601 ggtgtgtacc gtgcccggga cgagggcctc aaccgcgagg tggccatcaa ggtgatgttg
      661 aagagcctgg gagatgaccc catgttcgtg gagacgtttc agcgtgaggc gcaggccgcg
      721 gccaaactga accatccgaa catcgcgcag atctattcgt tcggtcagga aaaagggcaa
      781 ccgtatattg tgatggaact ggtttccggc ggcagcctcg ataagatgat ggcggcacag
      841 ggagcgatgg accccacggc cgtcatacag atcggcgagc agatcgccga ggggttgcgg
      901 gaggccgcag acgccggact ggtgcacggc gatgtgaagc ccgagaatat cctgttcgac
      961 aacgacaaga acgccaaact ggtggatttc ggtctggcgg ccatgaatgc cggaccgggt
     1021 aatgaagttt ggggtacgcc gtactacatc gcgccggaaa aggtacggcg gcagaaaagc
     1081 gatttccggt ccgatattta cagtctcggc gcaacgttgt atcacgcgat caccggggtg
     1141 ccgccgttcg acggcgagga tgccacggcc gtggtcaaag cgcgtttcga agggccgccc
     1201 aaaccgatgt cggaactccg taaaaacatc ccggacgaaa tcgaggcgct catccggcgt
     1261 atgctggagg tcgaacccca gatgcggtat ccgacctacg ggtcgctcct cggtgatatg
     1321 cggcgttacc tttccaaggt gaaaccggtc aatctcgcgt tgtcatcgaa aaaaatcatg
     1381 atcaagggca agttgtccaa gaccaccacc ggagtgccga cgataacggg aaacatgtcg
     1441 gacgacctgc ctcccggcat ggtgccggtg gacgcgattc cggaggagga tctcgaaagc
     1501 gaggaagagg cgaagcggcg cggccacagg atgatgctga tgatcctcgg cggagtgttt
     1561 ctgggcgtgg cgatgctggt tggaagcgtc ttcggcgtta aacactattt gggggtaaaa
     1621 cgggaaaaga ccgtgcgcgc gataaacgaa agccgtcaag gcaaggcgct ggcttcgatc
     1681 gcgaaatccg tggcacaggc gagaacgttg ccggaacggt tgagcggata tccgcaggag
     1741 gcgatggaaa cagcccaggc ggcagccgac gcggtggtcg cggtgttggg cgagacggtg
     1801 cggacgagca tgaccgtgcc cgagccgacg ttcgcctggg atgcgcaaga gagcgcagac
     1861 gcgcaaccgc catccgcacc gggcgaaacg gctgcggccc ccgcagtctc gaatgcggtg
     1921 ccgcaaggcg gagcggcgga ggtcaccaac accgttgccg cggcagggac caaccttgtg
     1981 acagaagcgg ggaccaatgc gtccgtcacg gctgcggcgc ccgcggcgcc cgcggcaccc
     2041 gcgccggaag ccgtgccagc agcgggaagc gatgatccga ccgcgccgcg ggagggcgac
     2101 gcgcatccgg tggtggccat cgtgcggggc atgttcgcgg aggcgtacgc cgtgaaaggc
     2161 ggggtgctgc tcgctgaaca tttggtaaag aaaatcgagg ccgaggcgaa agcggcggag
     2221 ggcatgaccg acttggagca gacggaagcg ctggtcaagc acgcgaatgc gctggtcgag
     2281 aaggtgaacg gcttggcgta cgtcaaagag gtggcggatg tgccgcgtaa agtctcgcaa
     2341 ctgaagaaga ccctcgagag cgtcaagacc gacaccgcct ctctggcgga gatgaaacgg
     2401 cgggaggcgc aggaacagga gaaacggcgc aaggaggaag agatcgccga aaagaagcgt
     2461 caagagaaag aagcccatca gaaaaaggtg gaagaggaga aggccaaagt ggcggacgcg
     2521 gaactcgcga accgcgatat gctcaaggcc ttgcagttcc gcgaggccgt gaatgcaatc
     2581 cgggagttgc gtggtgggct ggagaccgac gagggaggaa tcgcggccag tatcgccatc
     2641 gaccggatca accgcatgcg ggatttccat aattacctga tcgctcaagt caaaggcttc
     2701 aaatccgcgc gcggctggtc agtcgacgcg gcggacgcga agaccctctc ggtgggcggc
     2761 ataaaaatcc cgtgggctga cgtttacaac aaacgcccgg agatcatcgc cgaactgatc
     2821 aacggcctgg tgcggagcga acaggcgacc aagaccctga agctgagcga gaaaacccgg
     2881 ttgatgacca gtgcggcgtt gtgcctcaat ctgttttacg gggagatggc ttcggctcag
     2941 gaactggcca agcagatcgc gctggcagcg gctcagcagt ttgagatcga ggccgacacg
     3001 atcaaacagc tcctccccgg gttcttcacg gagtgacatg cggtacatca gcacccgagg
     3061 cggtggcgag ccgctgacat tcaggcagac cctgctgacc ggtttggcgc gtgacggcgg
     3121 actgttcctg cccgaaacct tcccggacgt gcgcgaccgc ctgaatgcgt ggcgcacgct
     3181 gccctatccc gaattggcct ttcagatcat ccgccttttc gcggacgaca ttccggagga
     3241 tgcgctgcgc cgtatcgtgg aacaaagcta cgcggggttc cgccaccatg aggtctgccc
     3301 cgtggtgccg gtgggaccgg tacgcatgct ggagctgttc cacggaccga ctctcgcgtt
     3361 caaggatgtg gccctgcagt tcctcggcaa cctcttcgag tacgcgctgg ccgagaccgg
     3421 cgcggagatg aacatcgtgg ccgcgaccag cggcgatacc gggagcgccg ccatctacgg
     3481 agtacgcgga cgtgaacgga tccgcatctt cgtgatgcac ccggccggcc gcgtgagccc
     3541 ggtgcaggaa cggcagatga ccaccgtgct cgacgcgaac gtgcacaacc tcgcggtgga
     3601 aggcacgttt gacgactgcc agaacatggt caagtcgctg ttcggcgatt tggcattccg
     3661 cgacacgttc cggctgggcg cggtgaactc gatcaactgg gcccgcgtgc tcgcccaaat
     3721 cgtctactat ttctatgcgg ccttccgcac acaggaggct accggtgccg cagcggtgcg
     3781 tttcagcgtg ccgaccggta atttcggcga tatcttcgca gggtatgtgg cgtaccggat
     3841 gggactcccg gtggatcagc tggtgttggc cacgaacgag aacgacattc tcgcgcgcta
     3901 tttcaacacg ggagtgtaca gcctgggcga ggtcgcgccg accctgagcc cctccatgga
     3961 cattcaggtg gccagcaact tcgagcgtta tttgtactgt ctggcgggcg gggacgcagc
     4021 catgctgaga ggatggatgg gcgccttcaa ggccgagggg actctgcgcc ccgatttgcc
     4081 gccagacccc gtgatcgttg ccggacgcgg cgacacagag atgacgctga ccaccatccg
     4141 cgccttctgg gagacccacg ggtacctgct cgatccgcat acggcggtgg gtgttgcggt
     4201 cggcatgcgt ttcatgcatc cggagatccc gctgatctgc ctggccacgg cgcatccggc
     4261 gaagttcggc gacgcgatcc tgcgcgcgac cggacaagat ctggcgcatc atccgatcct
     4321 cgacgcgctg gccacgctgc ccacgcgcgt gcgcaccgtg ccggccgacg tggacgcggt
     4381 ggcaaagatc gtcgcggcga ctgtccgggg agacggatga tcgatcccgc cacatttcgt
     4441 tgccgtcagt gtggggcgtg ttgccgcgta cccggttacg tcgcgctggc cgagggcgag
     4501 acagaggcaa tcgccgcgtt tctcggaatg gacgtgtacg ccttcacctc ccgctacaca
     4561 acgctgacct tcaaccgcgg gaccctctcg ctcacggaac gcgaagacgg acggtgtgtg
     4621 tttctcgaag acgacaacac ctgcctcatc cagccggtca agcccgcaca gtgcaaaggg
     4681 ttccccttcc tctggagcac ccccgcgctg gaacacgtct gcgcggccat acgacccgca
     4741 cgcgattcct gatccggacg ttatttccgg gagcccccga acagctcgtc cggcacggcg
     4801 ggcgcaggct ccggcaggcc tacttcaatc gctgtccgtc ggccaacagg cgcgtctgga
     4861 gtttggcgag gtccacctcc tgcaccgcga catcgtcctg aatcgccaac gccgcggctg
     4921 tggcagccga ctgtcccaag atcatgaaga ccggctccat gcggatcgag ccaaaggcga
     4981 tgtgggtcgc agagacgcaa accggcacga gcaggttggc gcactcctta cgtttgggaa
     5041 cgatcgcgaa gtacccgacc ggatagggtt tgagccaccc gagttccaca tcaccctcat
     5101 tctgaacata tccttgggcg tcgatgtacc gctgcacgtt gtgggagtcc atgccgtatg
     5161 cggccaatcc gacgggcgct tcggcacgga ccttcccctg gcaatggtgc tgcgtcatca
     5221 cgagatcgct caccatgcgc cgcgcctccc ggacataaag ctgctcctgc catccccctc
     5281 cctccgtgaa ctcgtcccgg cacatgcccc agcgcgacac ctctttccga acgtgttccg
     5341 gaacacgggg atggttagcc agcgtccaca tcagaccttg ctggtacagg aggtgacggg
     5401 cggcgatctt ctcgcgttcg gcataggagg cttcgggata atcgtagttc tgcccgatga
     5461 agtccgtgga aaacccgccg tagttgttgg tgtcggtttt gcggttgggc atatccgaat
     5521 tgatccagat cccttgtcgc cccgcctcga agttccgcaa caacaactcg aaccacaagg
     5581 gatcataccc ttccggtttg acgaagggaa tgcggttttc cggatggtcg gtcagacaca
     5641 tgcggtagca gtaggcctga acgcgtttgt cccccgcacc ctccttgccg ggccccgtcg
     5701 gatcgatgaa cggcagcagc ccgctcgcgg cattgccctt ctcgacataa ggatcgactc
     5761 cgggaagaaa gttgtggtgc ttgtatttga gatcgatctt ggcctgcaca ccattgtagt
     5821 cctcgccgta ctgcgcatta ctctcgcggc cgaccgtata actgaccttc gccgctgcca
     5881 tcagatcgcc ctcataggtc gtgtcgataa acatctttcc ccgaaacacc cgcccgcttt
     5941 ccatgcggat cgagaggatc cgccacggaa ccgtgcgcgt cagggccacg cccgacgcac
     6001 ggtcgagacg ttccccccgg acaaccggaa tggcattcgt gcggacccaa gcttcgtaaa
     6061 tcttgagggc cacgctcggc tcaaaggtcc acatcgcgtc ttcgttttga tccgttcgcg
     6121 tctggcctcc gtcccggtac tgttcccgct tctgccagat ccaagcggcc ggctgggcgt
     6181 accaggcacg gaccgcgcgg taaaattcac gcgcgatgcc gccgaccgcg accttgttgc
     6241 cgatatcggt ctgccccaac ccgcccgtgg tcaacccgcc aatacgttgc gtcggttcga
     6301 tgaccaccac atccaaagcc atgcgccggg cctgcaccgc ggccgcgatc ccgcccgacg
     6361 tgccgccgta gacgaccaga tcccagacct tcccttccgc tgccgtctcc tgcccgatca
     6421 gttgtgacgc aaacaacaaa aggatgcttc cgcataaaaa acgttttctc atgatgctcc
     6481 cttcttgatc tatcattcca ttaaaaaacg cattgtccct gttcgggggc gcttcgtcaa
     6541 ccttgatttt ggcctttcgc ggaacaagtt taggacatga ttaacatgat gttgttcaat
     6601 cctgaaaatc ctgtcatcct gtcaatttat cggacatgat tttccgctgc gcgggcgcat
     6661 atatccatga ttaacacgat gttgttcaat cctgaaaatc ctggatacat gcgcccgcgc
     6721 agcgggaaat cctggcaatt tataggcaag gagtttttca cttctcccgt cagaccttat
     6781 gt
//
LOCUS       DAOSLE010000139         6740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10272_length_6740_cov_7.049364, whole genome shotgun sequence.
ACCESSION   DAOSLE010000139 DAOSLE010000000
VERSION     DAOSLE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10272_length_6740_cov_7.049364"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1332
                     /locus_tag="PK576_08800"
     CDS             229..1332
                     /locus_tag="PK576_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HOE61729.1"
                     /translation="MRMEKWAGAALALAAAIFAGGCSINRTSSFKWLAFPLSWRSAPS
                     DPTQTLREKAESGDPDSQYKLGFSLLYGEDMPRDAESGLVWLKRSGENGYLKAQSALG
                     LLFLKGVPDIDRDDREAAVWLEKAAAQGHVVSQSSLGALLLSQTSSVHDSEAGVVWLK
                     RAAGQGDAPAQVALGQMYRNGYVVAQSDTESVRWYRKAAEQGNTGAQTQLGIAYYRGK
                     GVLQSFDEARRLFEAAAFRDDALAMSNLGVMASRGEGMKQDAELALSWFRKAAEGGHA
                     DGQNNLGAAYYKGIGVPQDYGAALAWFRKAADQGHASAQFRLGLMLEAGQGTDPDTSE
                     AAVWIKRAADQGFPPAVNRMRERGAAPAPDPTP"
     gene            complement(1343..3208)
                     /locus_tag="PK576_08805"
     CDS             complement(1343..3208)
                     /locus_tag="PK576_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61730.1"
                     /translation="MRGGIICALLTVCLAATGRTVPAVMYVDDTAGSDAAEGLTPDRA
                     WKTLDRVNREPLIPGDRVLFRRGGVWRGQLVPRSGTNGAAVTYGAYGTGAKPVLQGSV
                     ARDRREEWVCLHPGIWATPVYEPEALDQLADLKDSRWSLHAEASARAHLSRREEDGRG
                     FNRVVCDAPGSARNHIQLWGAELDGFPSPVLLRLRVRSSIPFRLDEVEALLNRPPYTR
                     TRSGHVRSALIGKEWRTVEAVLYETATGSGAHLHLSLGEKIPAGAVFDFEPLGLWRLR
                     PETRHAPIPCDVGILLLDHGVEWGVKKWRPEDLVNRLDYWYDGEGQRVVLVCETNPAV
                     RYASIELALTRTIVLQANCRDVTYEGLAVRYGGAHGFGGSRTQRITIRQCDLSWIGGG
                     LQDWRKRKGGERFPVRYGNAIEFWNGAEDHVVESNRIWEIYDAALTNQGHGDDSRQMR
                     IRYRHNVIWNAEYSFEFWNRPETARSEHILFEYNTCVDAGSGWAHKQRPDPNGAHLMF
                     YLNSSATTNVVIRNNLFVGTTEVCMRMENDWRGGLSMRNNLYWPGDKPFLRWLGKTFF
                     EAGGFTRYQRELALDSNSLLAEPKFMNPAARDYRLCPDSPGARLATDGGPVGARW"
     gene            complement(3217..4557)
                     /locus_tag="PK576_08810"
     CDS             complement(3217..4557)
                     /locus_tag="PK576_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61731.1"
                     /translation="MHTIHIPEALNDFDAHVRRDALRKVCGETRFPEPGTNFNMHCHS
                     FFSYNAEGWSPSCVAYVCRAQGLCAAGLCDFDVLDGLEEFSDAGLSLGLRTAVHLETR
                     AYVPEFAEAEISSPGEPGVTYIMGCGFIRLPDPDTAQARTLARLRDGAQERNLALLSR
                     VNAALPAIAVDYARDVLPLTPMGVATERHIVRAYCNRARTVMSDPAERATFWSPVLKC
                     GTEAFAALDANVPKMEDRVRNALAKRGGLGYIQPTAQTFPLADDFIRWVLACHAIPTV
                     TWLDGTSDGEADCERLLDQMTAKGCEALNIIPDRNWNLSKPDEAALKRAKLDAMVNAA
                     IRRDLPINIGTEMNKGGLPFVDDLAGPVLSRYADAFTQGMHIMVGHTVLGRYADAPYI
                     GARAQSEFTDRKRRNAFYAAVGALPALSLSQAEALSSAGPDKAFAMLADAAAKK"
     gene            4659..5291
                     /locus_tag="PK576_08815"
     CDS             4659..5291
                     /locus_tag="PK576_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61732.1"
                     /translation="MVFCFVLLLAVAVAGGVAGATPATFGAAAVSAALATWLLRADRG
                     VPRANHVTVTLDPVAPDSGMLLEGVREVLRRVHGTDRPARLRIQTRRAAADRPRLQLG
                     LTRDGDLDLIHDLRHTPLKQPGVWIADHPLPLNLSHVRCTTLRLTPAGGGRVRVRLTP
                     YYAWPSPPVWILLTAGIAAACLFDQAELLAGLLGFAAQAYLLRYQNECTS"
     gene            complement(5220..6038)
                     /locus_tag="PK576_08820"
     CDS             complement(5220..6038)
                     /locus_tag="PK576_08820"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOE61733.1"
                     /translation="MTRMYTPTGDAGAAALLAFVTAAIREGAAVAVAPEHVPVLTRFA
                     GCRTHGRGAAAGRYVFRAPRPERPVFTTAERVLAGFLLIALLPLLGLIALLILVTDGP
                     PVLFRQIRFGEDGRPFVFLKFRTMCRASEWLHAHLQHRQTEPGRLFKLENDPRVTRFG
                     ALLRNTFLDEFPQLLNVVRGEMRLVGPRPLPQSDQHHYTRPEHAMRLKGRPGMTGLWQ
                     VAGRNRRTFDEMCLLDLYYLGNRSVTLDLWVLGRTLILIAQQIGLGGKAQQPGE"
     gene            6132..6626
                     /locus_tag="PK576_08825"
     CDS             6132..6626
                     /locus_tag="PK576_08825"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOE61734.1"
                     /translation="MLSPDFHAHFMRQALREAARAEAAQEVPTGCVIIRRPSDPTISL
                     AAVSVLARAHNQTEMLCDATAHAEMIALTQASAALGDWRLLDTILYVTKEPCPMCAGA
                     IVLARVPLVVWGVSDPKRGGQSVFSILNHPGLNHRPETLAGVLETECRAALQTFFRSR
                     RAED"
BASE COUNT         1288 a         2212 c         2006 g         1234 t
ORIGIN      
        1 atactaccat agatgtggtt ttacaaccga tttcatctgc ggatccacgt ccgttttttt
       61 tcataatgtt gtgtatccaa tacctgccta ttcttgcaac cggattgacc gctcggggcg
      121 aaaaaggttt aatggaccgt accgcgcagg atctgccgcg agacggggac gcgtccggtg
      181 tccccaatgc ttggcggaat gcttgcgtgc agtcggtcag gaggatgcat gaggatggag
      241 aagtgggcgg gtgccgcttt ggcactggcg gccgcgatat tcgccggggg gtgttcaatc
      301 aaccggacgt cgtcgttcaa atggctcgcg tttcctttgt cgtggcgctc tgcgccgtcc
      361 gatcccacgc aaaccctccg cgaaaaagcg gagtccggcg accccgattc gcaatacaag
      421 ctgggcttct cgctgcttta cggtgaagac atgccgcgcg atgcggaaag cggtctggtc
      481 tggttaaaac gctcgggcga aaacggatat ctcaaagccc agtcggcact cggcctgctg
      541 tttctaaaag gcgtgcctga tatcgaccgg gacgaccgcg aggctgccgt ctggctggaa
      601 aaggcggcgg cgcagggtca tgtggtgtca caatccagtc tgggtgccct gctcttgtcg
      661 caaacaagtt cggtgcatga ttccgaagcc ggtgtggtct ggctgaaacg cgcggccggg
      721 cagggtgacg cacccgcaca ggtggcattg gggcaaatgt accgtaacgg ttatgtggtc
      781 gcgcagagtg acaccgaaag cgtacgctgg taccgcaaag ccgccgaaca gggaaatacg
      841 ggtgcgcaaa cgcaactcgg catcgcgtat taccgcggca aaggcgtgct gcaatcgttc
      901 gacgaagccc gccgtttgtt tgaggccgcc gcgtttcggg atgacgcatt ggcgatgtcc
      961 aacctcgggg tgatggccag tcgcggcgag ggcatgaaac aggatgcgga gttggcgctg
     1021 agttggttcc ggaaagccgc cgaaggcggg catgccgatg gccagaacaa tctcggcgcg
     1081 gcctactaca agggcatcgg cgtgccgcag gactacggtg cggcgctggc gtggttccgg
     1141 aaagccgcag accagggaca cgcgagtgcg caattccggc tcggcctgat gctggaagcg
     1201 ggtcagggaa cagacccgga cacgtccgag gccgcagtct ggatcaagcg ggcggccgac
     1261 caaggattcc cgccagcggt taaccggatg cgcgagcgcg gcgcggcacc cgcgccggat
     1321 ccaacaccct gacgcgcgga tctcaccagc gcgccccgac cggtccgcca tccgtggcga
     1381 gccgcgcacc ggggctgtct ggacacagcc ggtagtcacg ggccgcggga ttcatgaact
     1441 tcggttccgc cagcagcgag ttcgagtcca acgccaactc ccgctgatag cgcgtaaacc
     1501 ccccggcctc aaagaacgtt ttgcccaacc agcgcaggaa cggtttgtct cccggccagt
     1561 acaggttgtt ccgcatgctc aaccctccgc gccaatcgtt ctccattcgc atacagactt
     1621 ccgtggtgcc gacaaaaagg ttgttacgga tcacaacgtt ggtcgtggcc gaagagttga
     1681 ggtaaaacat caggtgcgcg ccgttgggat caggacgctg cttgtgggcc cagccggatc
     1741 cggcatcgac gcaggtattg tactcaaaca gaatgtgttc tgaccgtgct gtttccggcc
     1801 ggttccagaa ctcgaatgaa tactcggcgt tccaaataac gttgtggcgg tatcggatgc
     1861 gcatctggcg cgaatcgtcg ccatggccct gattggtcaa cgccgcatcg tagatttccc
     1921 agatacggtt gctctccacg acatgatctt ccgcgccatt ccaaaactcg atggcgttac
     1981 cgtaccgcac cgggaaacgc tcacctcctt tcctcttgcg ccaatcctgc aatccaccgc
     2041 cgatccaaga cagatcgcac tgccggatgg tgatgcgttg tgtccggctc ccgccgaaac
     2101 catgtgcgcc gccgtatcgc accgcgaggc cctcatacgt gacatcccgg cagttcgcct
     2161 gcaagacgat ggtccgcgtc aacgccaact cgatgctggc ataccgtacc gccggattgg
     2221 tctcgcaaac cagaaccacc cgctgtcctt ctccgtcata ccagtagtcc agccggttta
     2281 cgagatcttc gggacgccac ttcttcaccc cccactccac accatgatcc agaaggagaa
     2341 tcccgacatc gcagggaatc ggcgcatgcc gggtctccgg gcggagccgc cacagcccga
     2401 gtggttcgaa atcgaaaacc gcgcctgcgg gaatcttctc cccaagcgag agatgcagat
     2461 gggcgccgga tccggtcgcc gtttcgtaca acaccgcttc gaccgtccgc cattctttcc
     2521 cgatgagtgc ggaccggaca tgcccgctgc gggtacgggt gtacggtggc cggtttagca
     2581 aggcttcgac ctcatccagt ctgaacggga ttgaggaacg cacacgcaga cgcagcagga
     2641 caggcgaggg gaacccgtcc agttcggccc cccagagctg gatatggttg cgcgcgctgc
     2701 ccggtgcgtc gcacaccacc cggttgaacc cgcgcccatc ctcttctcgg cgggaaaggt
     2761 gcgcccgcgc actcgcttcc gcatgcaggc tccagcgcga gtctttcaga tccgccaatt
     2821 gatccaacgc ttccggttca taaaccggcg tggcccagat tccgggatgc aggcagaccc
     2881 attcctcccg ccgatcccgg gccacggagc cttgcagcac cggtttggca cccgtgccat
     2941 aggcaccata cgtcaccgcc gcgccgtttg taccgctccg gggaacaagc tgacctcgcc
     3001 acactccgcc ccgtctgaac agcacccggt cacccgggat cagcggctcg cggttgacgc
     3061 gatccaacgt tttccacgcc cggtccggcg tcaggccttc cgcagcatct gatccagccg
     3121 tgtcatccac atacatcacg gcaggtacgg tgcgtccggt ggcagcaaga cacacggtca
     3181 gcagcgcaca tatgatcccg cctcgcatca ccaacctcat tttttcgccg ccgcatcagc
     3241 cagcatcgcg aacgccttgt ccggtccggc ggacgacaag gcctcggcct gagagagcga
     3301 aagtgcgggc agggcgccaa ccgccgcgta aaacgcattg cgccgtttcc ggtcggtgaa
     3361 ctcgctctgc gcgcgcgcgc cgatataagg ggcatccgcg tagcggccga gaacggtgtg
     3421 ccccaccatg atatgcatac cctgcgtaaa agcatccgca tagcggctca gcacgggtcc
     3481 ggccagatca tccacgaatg gcaggccgcc cttattcatc tctgtgccga tgttgatcgg
     3541 caggtcgcgc cggatggcgg cgttcaccat cgcgtccagc ttcgcgcgtt tcagggccgc
     3601 ttcgtccggt ttcgaaagat tccagttgcg gtcgggtatg atgttgagcg cctcgcaccc
     3661 tttcgcggtc atctgatcca gcagccgctc gcaatccgcc tcgccgtcgc tggttccgtc
     3721 cagccaggtc accgtgggaa tcgcatgaca ggccagcacc cagcggatga agtcgtctgc
     3781 caatgggaag gtttgcgcgg tgggctggat atacccgaga cctccgcgtt tcgccaatgc
     3841 gttacgcact cgatcctcca tcttcggcac attcgcgtcg agtgccgcaa acgcttccgt
     3901 gccgcacttc agcaccggcg accagaacgt tgcccgctcc gccgggtcgg acatcaccgt
     3961 gcgggcgcgg ttgcagtagg cccgcacgat gtgcctctcc gtggccacgc ccatcggcgt
     4021 aaggggcagc acgtcacgcg cataatcgac cgcaatggcg ggcagcgcgg cgttgacccg
     4081 gctgaggagc gccagattgc gttcctgcgc cccatcgcgt aaccgggcca gggtccgggc
     4141 ctgcgcagta tccggatcag gcaaccggat gaacccgcac cccatgatgt atgtgacgcc
     4201 cggctcaccg ggcgaactga tctccgcctc cgcaaattcg ggcacatagg cacgggtctc
     4261 cagatgcacc gccgtacgta aacccagaga caagcccgca tctgaaaact cttccaaccc
     4321 gtccaacaca tcgaaatcgc aaagccccgc agcgcacagc ccttgggcac ggcagacgta
     4381 cgccacacag gaaggcgacc acccctcggc gttgtacgag aagaacgagt gacagtgcat
     4441 gttgaagttc gtgccgggtt ccgggaatcg cgtttccccg cagaccttgc ggagcgcatc
     4501 gcgccggaca tgggcgtcaa aatcattcag tgcctcggga atgtgaatcg tgtgcatagc
     4561 cgtttccttt cgcgacaaaa agcatagcag aaaattccga cgcgcggaaa cggtgttttc
     4621 cgcttgcctg aaaagcgtac cgcagattat ggtgtgacat ggttttttgc tttgtcctgc
     4681 tgctagccgt ggcggttgca ggcggcgtgg ccggtgccac gcccgcaaca ttcggggccg
     4741 cagccgtttc agccgcattg gccacttggc tgttgcgcgc ggatcgcggt gtgccccgcg
     4801 ccaaccacgt caccgtgact ctggacccgg tggcgccgga cagcggcatg ctgctggagg
     4861 gggttcgtga agttctgagg cgcgtgcacg gcaccgaccg cccggcccgc ctgcgcatcc
     4921 agacccgccg cgcggcagca gaccgcccgc gtctgcagct cggcctgacc cgtgacgggg
     4981 atctcgacct gattcacgat ctgcggcaca caccgctgaa gcaacccggt gtctggattg
     5041 cggatcaccc gttaccgctg aatctgtccc atgtgcgctg cacaactttg cgtctgaccc
     5101 cagcgggcgg cggccgggtt cgcgtccggc tcacgccgta ttacgcctgg ccctccccac
     5161 cggtctggat cttgctgaca gcgggcatcg ccgccgcctg tctcttcgat caagcggaac
     5221 tactcgccgg gctgctgggc tttgccgccc aagcctatct gctgcgctat cagaatgagt
     5281 gtacgtccta acacccacag atccagtgtt acggaacggt taccgaggta gtagaggtcc
     5341 agcagacaca tctcgtcaaa cgtccggcgg ttacgacccg ccacttgcca caaccccgtc
     5401 atgccggggc ggcctttgag ccgcatggcg tgctccggac gcgtgtaatg atgttggtcg
     5461 ctctgcggta gaggacgtgg cccgaccagc cgcatctcac cccgtaccac attcaggagt
     5521 tgcggaaact catccagaaa cgtgtttcgc agtaacgcgc cgaagcgggt cacgcgcgga
     5581 tcattctcca gtttgaatag gcgtcccggc tcagtttgcc tgtgctgcag gtgggcgtgc
     5641 aaccattcgg aggcgcgaca catcgtgcgg aatttcagga aaacgaatgg acgcccgtcc
     5701 tccccgaaac gaatctgccg gaacagaacc ggcgggccgt ccgtgacgag gatcaacaaa
     5761 gcgatcagac ccaacagcgg caggagcgcg atcaacaaaa acccggccag caccctttcc
     5821 gcggtcgtga acacaggtct ctccgggcgg ggcgcacgaa acacgtatct tcctgcagca
     5881 gccccgcgac cgtgagtccg gcaacccgca aaacgagtca ggaccggcac atgctccggg
     5941 gccacggcaa ccgccgcgcc ctcgcggatc gccgccgtga caaatgccaa cagcgcggcg
     6001 gctccggcat cccccgtcgg tgtgtacatg cgcgtcatcc ccatgccccg tattatcccg
     6061 tttcggtccg atcgcatcaa gaacggaaag cccgccttta catcacgccg ccgtccgcct
     6121 acaatcccgg catgttgtcg cccgatttcc atgcccattt catgcgtcag gctttgcggg
     6181 aagcagcgcg tgccgaggcc gcgcaagagg tcccgaccgg ctgcgtaatc atccggcgtc
     6241 catcggaccc caccatttcc ctcgccgcgg tcagcgtgct ggcccgcgcc cacaatcaga
     6301 cagagatgct gtgtgacgcc accgcgcatg cggaaatgat cgcgctgacc caagcttcgg
     6361 cggcgctcgg cgactggcgc ctgcttgaca cgatcctgta cgtcaccaaa gaaccctgcc
     6421 ccatgtgcgc cggcgccatc gtgctggccc gggtgccgct tgtggtttgg ggcgtgtccg
     6481 atcccaagcg cggtggtcag tcggtgttct cgatcctcaa ccacccggga ctcaaccatc
     6541 gtcccgaaac gctggcggga gtgctggaaa ccgagtgccg cgccgcgctg caaacgtttt
     6601 tccgcagtcg ccgcgctgaa gactgacccc aaaagagcac ttgacctttc agctcaaaag
     6661 gagcagttga acgggtagaa ttctagcaaa gccccgtaaa cattgggttt tgtcgcggtc
     6721 cccgccgcac cgtattgaga
//
LOCUS       DAOSLE010000140         6703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10360_length_6703_cov_9.530686, whole genome shotgun sequence.
ACCESSION   DAOSLE010000140 DAOSLE010000000
VERSION     DAOSLE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10360_length_6703_cov_9.530686"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2112
                     /locus_tag="PK576_08830"
     CDS             313..2112
                     /locus_tag="PK576_08830"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOE61735.1"
                     /translation="MPMSRMSFHRVAICFALLLATRAILAPAAPFLPLSLTDAANTGF
                     SDVEEGDRKGGWLDLGGNDLSVLPAGLLTVSSRLPFQILDSKKNPDRTCVVLGGPKRP
                     YLPSRAEIRVDGKTGAVLYLLHGAAWCPPPQQQKMAGVLTVEYADGTGKEYHVRCGRD
                     VGDWTKPDSYSNAARVWSAYNANTQVSLFASKFKLEAKPIRLIRLRSQECAWMVVAAT
                     LGDEISIKPIRRNLTLDKKYTAPPPFEKPLRDDLSDTRPKNIILIIGDGMGEGAQKLT
                     SLYQHKAEGRLAMQQLPVTGYLTTFSANSEVTDSAASSTAIACGHKTNNGMLGLTPQT
                     NTVLSVAALARESGRAVAILTSDAVTGATPSGFYAHVVRRGAYSDIAEFAAACGYDML
                     IGNANARAWFTPEAAGGQRTDTRNVLNEMTAAGYAVVTNAALFAAVPEGQRVVGFLDK
                     PTLEPETALGTLMDIALPRLARHDKGFFMMMECTITDAGGHGNNPEYTVRGTLQVDWA
                     VRRAVDFAKSRGDTLVLVTADHETGGLFCSLSRAVPDRLTICYTTTSHTGAPVPIHAF
                     GPGSKYFQGMNDNTDIAKTVAFLWRMRLSAPTR"
     gene            2172..2750
                     /locus_tag="PK576_08835"
     CDS             2172..2750
                     /locus_tag="PK576_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61736.1"
                     /translation="MRSEAAAKQSFGATAYAVRPGVCVACGRAAGRHERACVYCGERV
                     WRSFRNRAARAAWIAVPPVLLLWLTVAARPDWGAVCQTILDGGALSGYLFAAGAGLLL
                     LPLEDDDLVTASETERLQWQALSHAGAFLCGGYAALGSACLACGARVGPAVWVAVIAV
                     FACVALAPLLFRISWRAVAAAALWAAVWLGHG"
     gene            complement(2772..4520)
                     /locus_tag="PK576_08840"
     CDS             complement(2772..4520)
                     /locus_tag="PK576_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE61737.1"
                     /translation="MLTVFRAPAQTAVSNVVILIEATPLASLVRPGPEPLRDIPGLML
                     RQQGASAPQGDLSIRGSPFNGAGLLLNGLTLRNAQTEHWHADLSVPNIWTDTPTVLTG
                     MERFRAASGHPSGSVSLELAPLSETLRSITLGGGDKGALFGQAVFTETEPFRDGVAGA
                     SAFVSADRADQTDGYRDNDLVRGAAGARIHAANETMRSDLVTALSWREFGARGFYGTD
                     PSFPAAERKGDALAAGSLRFVQDPRQPARLSAAWLRTRDTYWLDRHNRTFYENDHTTD
                     FFVAHGDTRRTFNDTVSVDLRADGDLETLESRSLGNHTRSHASLAVLPNLTCGPLTWT
                     VGGALDLFSTDSPIPLPAAGVEWTVSERHTLFLSYTAAARQPSYTELNYESPSSLGNA
                     GLDRQRTQTAELGWKAEHGPFRWRTVLFHEEGRNIVDWRRTSPAGRWQAVNLETLRTT
                     GLAAEGVWEVDGNTDFGLNLLTVSKDTDTSGYASRYALDYPRAAADACVRHRLTRELS
                     LRLSQGVSRYATNHARQHDNWFWDTRAGVQWQPRQLPGLTLNAGIDNLLDDDFQIFPG
                     QETAGRCMYVSLTHVW"
     gene            complement(4720..6207)
                     /locus_tag="PK576_08845"
     CDS             complement(4720..6207)
                     /locus_tag="PK576_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE61738.1"
                     /translation="MTMTKKHIRILPVLVCAAWACVAEQKTALTCDVAVIGGGSAGFA
                     AAWSAAHLGSSVILVEKEPMLGGTSTLGGVNNWEPGMGGTGVPYRVYQRLRQIPGAAG
                     VYRFDRHLAWPKPWEKYTFPGGMIETDPCVSYAHTLRRHGSGMSNEAWFREHCRGVIF
                     EPDAMAETMLAMLNETGRCRVLLNTAFREARHADGMVSEVTLSDGAVVRAKIFIDATD
                     AVFCAQLGCELMTGRDRRADFNEPGAPPEPLMRLNGATLMYRITPLAKNEPDLIEPLP
                     EGIPEKCWWQGGFPVAFVGQYPKGDRFVNMLPTMRGEEYLELGPKKAYAECRRRVLAH
                     WHWYQKNYDEFRRFRLKSIFPVMGVRETRRVRGEYVLNQRDLIATLANQKHDDIIAIA
                     DHPMDNHGGGGPSGELKGPYGIPYRCLLPLKTTNVLIAGRAASFSAIAASSCRLSRTM
                     MQLGQAAGTAAHLSCSGNIPLREVQASEIRRVMAEHIDQFVQKCE"
     gene            complement(6319..>6703)
                     /locus_tag="PK576_08850"
     CDS             complement(6319..>6703)
                     /locus_tag="PK576_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61739.1"
                     /translation="ERQRLAIAVALAAQPDILLLDEPTSHLDLRHRVDLMALLLRLNR
                     DRKITILMVAHDLTLASQFFSRIILLSRGVKLADGTPAEVLRPDLLEAAYGCPVNVIP
                     LPDFAASCVLPMLVPEQTEEGERIL"
BASE COUNT         1230 a         1947 c         2271 g         1255 t
ORIGIN      
        1 aggctgtgag gcggtggtat tccgtggtta aatgatcgat cccgatcccg atagcgatcc
       61 cgatcccgat ttggatgaaa ccctgaaacc aaagagatgg gttttggaca aaggtgcgta
      121 ctcattggct tggccatgat ccgcatcggg gaggtgttcc ggggcatggg cgatccgaaa
      181 aaaagggttc cgcgttcccg cggttcctgc tagactgacc ctgttttgtt aaaccaaccg
      241 gagtgcgccg gatccggctc ataaaagggg gagccgggga agagggcgca cccagacgaa
      301 gaggggatca ttatgccgat gtcacggatg tcgtttcacc gtgttgccat ctgtttcgcg
      361 ttgcttctcg ccacaagagc gatccttgct ccggcggctc cgttccttcc gctgtcgctc
      421 acggatgcgg cgaatacggg cttcagtgat gtggaggagg gcgaccgcaa gggcggatgg
      481 ctcgatctgg gcgggaacga tctgagtgtg ctgcccgcag gtctgttgac ggtttcgtcc
      541 cgtctgccgt tccagattct ggacagcaag aaaaatccgg accgaacctg cgtggtgctg
      601 ggtggcccca agcgccccta tctgccgtcg cgggccgaaa tccgcgtgga tggaaaaacc
      661 ggcgcggtcc tctacctcct gcacggcgcg gcctggtgtc cgccgccgca gcaacagaag
      721 atggccggtg tgctgaccgt ggagtatgcg gacgggacag gcaaagagta tcatgtccgt
      781 tgcggccgcg atgtcggcga ctggacaaag ccggacagtt attcgaacgc cgcgcgtgtc
      841 tggagcgcgt acaacgcgaa cacccaggtg tcacttttcg cctccaaatt caagctggag
      901 gccaaaccca tccggctgat ccggttgcgt tcgcaggagt gcgcctggat ggtggtggcg
      961 gccacactgg gcgacgagat ctcgatcaag ccgatccgcc gcaacctgac gctcgacaag
     1021 aaatataccg cgccgccgcc gttcgaaaag cccttgcggg acgatttgag tgacacacgg
     1081 ccgaagaaca tcatcctgat catcggggac ggcatgggcg aaggcgcgca aaaactcacc
     1141 tcgctttatc agcacaaggc ggaggggcgt ctggccatgc agcagttgcc ggtcacgggc
     1201 tacctcacca ccttttccgc caactcggag gtcacggact cggccgcgtc ctccaccgcc
     1261 atcgcctgcg ggcacaagac gaacaacgga atgttgggcc tgacgccgca aaccaacacc
     1321 gttctttccg tggcggctct ggcccgggag agcggacgcg cggtggcgat cctcacctcc
     1381 gatgcggtga ccggcgccac gccctccgga ttttacgcgc acgtcgtgcg gcgcggcgcg
     1441 tattccgata tcgccgagtt cgccgcagcc tgcggttacg acatgctgat cggcaacgcc
     1501 aacgccaggg cgtggttcac gcctgaagcg gcgggtggcc agcgcaccga cacccggaac
     1561 gtgctgaacg agatgactgc cgccggatat gccgtggtca cgaacgcggc gttgttcgcc
     1621 gccgtgcccg aggggcagcg ggtggtcggt tttctggata agcccacgct ggagccggaa
     1681 accgcgctgg ggactctgat ggatatcgcg ctgccgcgtc tcgcccgcca cgacaagggg
     1741 tttttcatga tgatggagtg caccatcacc gacgctggcg gacacggcaa caatccggaa
     1801 tacacggtgc gcggcacgct gcaggtggac tgggcggtgc ggcgggccgt ggatttcgcg
     1861 aagtcgcgcg gcgacacgct ggtgctggtc acggccgacc acgagacggg gggcctgttc
     1921 tgttcgttga gccgtgccgt gccggaccga ctgacgatct gttacaccac aaccagtcat
     1981 accggcgcac cggttccgat tcatgcgttc ggtccgggct ccaagtattt ccagggcatg
     2041 aacgacaaca cggatattgc caaaaccgtg gcgttcctgt ggcgaatgcg cctgtccgcg
     2101 cccacgcggt gaggaggcga caccggtgtt ccttttgcgt ccgcaccgag cggcaaggga
     2161 atttctctgc gatgaggtct gaggccgctg caaaacagag tttcggagcg acggcgtacg
     2221 cggtacggcc gggggtatgc gttgcgtgcg gacgtgcggc cggtcggcac gaacgtgcct
     2281 gcgtctattg tggcgagagg gtttggcgtt cattccgcaa tcgcgctgcg cgcgcggcgt
     2341 ggattgccgt gccgcccgtg ttgttgctgt ggctcacggt tgcggcccgg cccgattggg
     2401 gtgccgtatg ccaaaccatc ctcgacggcg gtgcgctttc aggatatctg ttcgcagcgg
     2461 gggcaggcct gctgctgttg ccgctggagg atgatgatct ggtgaccgcg tcggagacgg
     2521 aacgactcca atggcaggcc ctctcacatg cgggcgcgtt cctgtgcggg gggtatgcag
     2581 cgttggggtc ggcgtgtctc gcgtgtggcg cgcgcgtcgg acctgcggtc tgggttgcgg
     2641 tcatcgccgt attcgcttgc gtcgcactcg cgcctcttct cttccggatc tcttggcgcg
     2701 cggtggcggc cgcagcgctt tgggctgcgg tgtggctcgg acacggatga cgaacggcgg
     2761 ttgtgccgga cttaccagac atgggtgagg gagacgtaca tgcagcgccc tgcggtttcc
     2821 tggccgggaa agatctggaa atcatcgtcc agcaggttgt cgatgccggc attcagggtc
     2881 aggccgggta gctgacgcgg ctgccactgc acccctgcgc gggtgtccca aaaccagttg
     2941 tcatgctggc gggcgtgatt ggtggcataa cggctgactc cttgactcaa ccgcaaagaa
     3001 agctcgcgcg tcagacggtg gcgtacgcat gcgtcggcgg cggcgcgggg ataatccagc
     3061 gcgtaacgac tggcataccc ggacgtgtcc gtatccttgg acacggtcaa caggttaagt
     3121 ccgaaatccg tgttaccatc cacttcccag acgccctctg cggcaaggcc cgtggtgcgc
     3181 agagtttcga gattgaccgc ctgccagcgg cctgcgggcg aggtgcggcg ccagtccacg
     3241 atattccgcc cctcttcatg aaacaacacc gtacgccatc ggaaaggtcc gtgctcggcc
     3301 ttccagccga gttcggcggt ttgggtacgc tgccggtcga gaccggcgtt gccgagggaa
     3361 gaaggcgatt cgtagttgag ttcggtgtat gagggttggc gggctgcggc ggtgtacgac
     3421 agaaacagag tgtgacgttc cgaaacggtc cattcgacac ctgcggcagg cagggggatg
     3481 ggggagtcgg tggagaagag atcaagcgct ccgcccacgg tccaggtgag cggaccgcag
     3541 gtgagattcg gcagaacggc gagcgaggcg tggctgcggg tgtggttgcc gagcgagcgg
     3601 ctctccagcg tttcgagatc cccgtcggcg cgcaggtcta cggagacggt gtcgttgaac
     3661 gtgcggcgcg tgtcgccgtg cgcgacaaaa aaatcggtgg tgtggtcgtt ctcgtaaaac
     3721 gtccggttat gccggtcaag ccaataggtg tcccgtgtgc ggagccaggc ggcggagagg
     3781 cgggccggtt ggcgcgggtc ctgcacgaaa cgaagtgatc cggccgcgag cgcgtcgccc
     3841 ttgcgctctg cggcgggaaa actcgggtca gtgccgtaaa agccgcgcgc gccgaactcg
     3901 cgccaggaga gcgcggtgac gagatcactt cgcatcgtct cgttggcagc atgaatccgg
     3961 gcaccggccg caccgcgtac gagatcgttg tcgcgatacc cgtcggtttg gtcggcccgg
     4021 tcggctgaaa cgaaggccga ggcaccggca acgccgtcgc ggaaaggctc ggtttcggtg
     4081 aagaccgcct gaccgaaaag agcgcccttg tcgccgccgc cgagtgtgat ggagcgcagg
     4141 gtttcggaga ggggcgcgag ctccagactc accgagccgg aggggtggcc ggacgcggcg
     4201 cggaaacgtt ccatgccggt caacaccgtc ggggtgtcgg tccagatgtt cggcacggac
     4261 aggtccgcgt gccagtgctc tgtctgggca ttgcgcagtg tcaggccgtt cagcagcaac
     4321 cccgcaccgt taaacgggct gccgcggatc gagaggtcac cctgcggcgc gcttgcgccc
     4381 tgctggcgaa gcatgagacc ggggatatcg cgcaacggtt cgggcccggg ccggacaagg
     4441 gaggccaagg gtgtggcctc gataaggatc actacattgg agacggcagt ttgagcgggg
     4501 gcacggaaca cggtgagcat cacaaacaat acggggaggg ggcgtaagac taaccggggg
     4561 aagtgcatga gagacgcgag attaaccggg ccgggaatga ttgtcaacca caagaaataa
     4621 aataggttac aatatggggg cgagcagaaa gccggggcgg gcaaagcccg caggagaagg
     4681 cggaagagac tcgttagtcg gttgcttttt ttgttatgtt cattcgcact tttgaacaaa
     4741 ttggtcgata tgctccgcca tcacgcgccg gatctcagat gcctgcactt cgcgtagcgg
     4801 gatgttgcct gaacatgaca gatgcgcggc ggtgccggcc gcctgcccga gttgcatcat
     4861 ggtgcgcgaa aggcggcagc ttgatgctgc gatggcgctg aaactggcgg cccgtccggc
     4921 gatcaggaca ttcgtggttt tcaacggcaa cagacaacgg tacggaatgc cgtaggggcc
     4981 tttcaactca ccgctcggtc cgccgccgcc gtggttgtcc atcgggtggt cggcgatggc
     5041 gatgatgtcg tcgtgttttt gattggcaag cgtcgcgatc aggtcgcgct ggttgagcac
     5101 atattcgcca cgcacacggc gggtctcgcg cacgcccatg accggaaaga tcgatttcag
     5161 ccggaagcgc cgaaactcgt cgtagttctt ctgataccaa tgccagtgcg ccaacacgcg
     5221 gcggcggcac tccgcatacg ctttcttggg acctagctcg agatactctt cgccgcgcat
     5281 ggttggcagc atgttgacaa agcggtcgcc cttcggatat tgaccgacga acgcaaccgg
     5341 gaagccacct tgccaccagc atttttcggg aatgccctcg ggcagcggtt cgatcaggtc
     5401 gggctcattt ttcgcgagcg gcgtaatgcg gtacatgagg gtggcgccgt tgagacgcat
     5461 gagcggctcc ggtggtgcgc cgggctcgtt gaagtcggca cggcggtcgc gtccggtcat
     5521 taactcgcaa ccgagctggg cacagaacac ggcatctgtg gcgtcgatga agattttcgc
     5581 gcgcacgacc gcgccgtcgc tgagcgtcac ctcgctgacc atgccgtccg cgtggcgcgc
     5641 ctcgcggaaa gccgtgttga gcagcacgcg gcaacggccg gtttcattca acatggccag
     5701 catggtttcc gccatggcgt ccggctcgaa gatcacgccg cggcagtgtt cgcggaacca
     5761 cgcctcattg ctcatgccgg aaccgtggcg gcgcagggtg tgggcgtagg agacacaggg
     5821 atcggtttcg atcatgccgc cggggaaggt atacttctcc catggcttgg gccaggcgag
     5881 gtgacggtca aagcgataaa cgccggcagc gccggggatc tgccgcaaac gctgatagac
     5941 acgatagggc acccctgttc cgcccatgcc cggctcccag ttgttgacgc cgccaagggt
     6001 ggaggttccg cccagcatcg gttccttttc gaccaggatg acggaggagc cgagatgtgc
     6061 ggcggaccat gctgccgcaa atccggcact cccgccgccg atgacagcga catcgcaggt
     6121 gagtgccgtt ttttgttcgg ctacgcatgc ccatgcggcg cacaccaaca ccggcaggat
     6181 tcgtatgtgc ttttttgtca tcgtcatggg tcctcaaccg tgttccctga tccgatagaa
     6241 ccgcaacgac ctgattgtct ctgatctgcg gcacgattgc aaagttaata aatatgctgc
     6301 gaagcttttg tcaccacact acaaaatacg ttctccctct tctgtctgct ctggcacgag
     6361 catcggcaac acacaggagg ctgcgaaatc tggcagcgga atgacgttga ccgggcagcc
     6421 ataggccgct tccagaagat cggggcgcag gacttcggcg ggggtgccat cggcgagttt
     6481 gactcctcgg ctcagcagga tgatccgcga gaaaaactga ctcgccaagg tgaggtcgtg
     6541 tgccaccatc aggatggtga tcttgcggtc gcggttgagg cgcagcagta acgccatgag
     6601 gtctacgcgg tgacgcaggt ccagatgcga ggtgggctcg tccagtagca ggatgtcggg
     6661 ctgtgccgcc aaggccacgg cgatggccag ccgctggcgc tcg
//
LOCUS       DAOSLE010000141         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10370_length_6698_cov_8.276381, whole genome shotgun sequence.
ACCESSION   DAOSLE010000141 DAOSLE010000000
VERSION     DAOSLE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10370_length_6698_cov_8.276381"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1022
                     /gene="nfo"
                     /locus_tag="PK576_08855"
     CDS             180..1022
                     /gene="nfo"
                     /locus_tag="PK576_08855"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOE61740.1"
                     /translation="MYFIGPHVSIGGGVANAPKNAKALGATGFGMFVKNQRQWTAAPY
                     AAADIEAFRRQMAADGYTAAQVMPHAGYLINLANPDETAHAKSMTALLDELRRCMALG
                     LDKLNLHPGSHLRLITPQAACERVAQSINRALNQTEGVTLVIENTAGSGGNLGAVFEE
                     IRTIIEGVDDKSRVGVCLDTMHAFAAGFDIRTRDGFLKTMEHFDQTVGMAYLRGLHLN
                     DSKVALGSRVDRHASLGAGLLGLDVFSCILTDARFENMPLVLETPDETIWEQEIRQLM
                     DMAG"
     gene            complement(1073..1837)
                     /gene="radC"
                     /locus_tag="PK576_08860"
     CDS             complement(1073..1837)
                     /gene="radC"
                     /locus_tag="PK576_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOE61741.1"
                     /translation="MEQPPCPSDQRTVYPDAAPPMRDLPVEMRPREELQRRGAENLPD
                     ELLLAILLRSGLPGKNVTELAREILLRYGGLAALSKTTFEELLHNRIKGLGKVKALEL
                     SAALELGRRAAQQGPRHDTPVIRDPEAAYRVLLPLTRTLQQEIFWTLLLDTKSRLIGQ
                     PIETSRGLLDSSPVHPREVFNKAVRYSAASMILAHNHPSGDPIPSKEDIEVTRRLVEA
                     ARILGIRVVDHLIVGKPSAVSPGYVSLRERNLVAFE"
     gene            complement(1812..2759)
                     /gene="miaA"
                     /locus_tag="PK576_08865"
     CDS             complement(1812..2759)
                     /gene="miaA"
                     /locus_tag="PK576_08865"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE61742.1"
                     /translation="MTLAWTLAGATATGKSAAAQLLAERLEIAILSADAMLVYRGMDI
                     GTAKPSAAERARVTYFGVDLVTPDQPFSTGAWLREAARAVAHTNAADSDGKAVPSLLV
                     VGGTGLYFKALTRGLDSAMADPARRAHWQARLETEGVGALREALAALDPQALASLSDP
                     SNPRRLIRAIEQLESLRSLPQNWKQPAPDAPLTVLRMPREQLHRRIRTRVEAMFAQGL
                     VEEVRSLRRHYPVWSASAAQAIGYAESCALLDGTLSQAEAFERIVIRTRQLAKRQETW
                     FRHQTQAVWIDTDERDPPERIAERILKAWRQHGATPLPF"
     gene            complement(2767..3255)
                     /gene="lspA"
                     /locus_tag="PK576_08870"
     CDS             complement(2767..3255)
                     /gene="lspA"
                     /locus_tag="PK576_08870"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE61743.1"
                     /translation="MRVLLLAALLVAADQISKYWAVVRLQAVRSVSVIPGLFSFSYVE
                     NQGAAWGMLAGRQAFLIGFSIATLIFMIWRRRALFGALRGRTVIMSLLFGGVVGNLID
                     RLRLGHVIDFLDFHWGDAHFPAFNVADSAICCGVFLYLIMQWRHDRHMARDQMTEPET
                     GD"
     gene            complement(3265..4011)
                     /locus_tag="PK576_08875"
     CDS             complement(3265..4011)
                     /locus_tag="PK576_08875"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOE61744.1"
                     /translation="MATTRKTKTGSTKKSPPKPVQKATAKRAAPAKRAAPAKRATAAK
                     RAAPAKRAAPAKRATAAIAKKPAARKPAAQAKPARTAKTPPITQSRTVVRKSAVKRGT
                     STEAPVKKGPPSYKLVAKKPVRGSPHFSKRDLNAFKRDLLSMRERITGQSGAMRHAAL
                     QRTDETNPEEDGTNAFMRLQTLEQVSSQLQTITNIDEALRSIEKGNYGVCDTCGELIS
                     KLRLAVLPFAKNCIRCQSEMEKQFRFRGRR"
     gene            complement(4150..6291)
                     /locus_tag="PK576_08880"
     CDS             complement(4150..6291)
                     /locus_tag="PK576_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOE61745.1"
                     /translation="MMDAAMTFEELETRFFSLLEIRPLPVTDLLPLLQAITTTTDAGK
                     AEEWTMLLIEELVDATDFRGLFLTVMDQVEPLSCALAPDAILRLFKQTCPDRLTDVML
                     DVAQFGEKPLYEALKRLGTLLALEPGTQVIDPAWGFGVIQEADSFYKRFIVDFTRKPR
                     HEIPFATACDSLVPAPQDHLLTVRHNDPGGFAQIVREQPGELLRLTLRSYGDLTAVRI
                     EDLLVQEKILAPDEWKGFWDAARKALKQDPLVVFPAKKSEPLRLLTEAESYGDAWFAT
                     FSATKNPERLLTQAIELETAGRIENLDAAQRAILEERLHFALGAAHQSDAALYARLAV
                     MLSRFGFLTPPTDQLRAHLWEEHRFLRASEALTVKDTADLATFLLAEGSAAAQRLLDA
                     LPQMPFSLLNEVLNRLNTSAEAEEACRRLLTQPKAPPTLVYWAFRNRDTPGWRLPPLH
                     ELLDHAIQIVESRLSGEALRMQNQLKRLFGQTSWLESVFAELDMPQRQRIFERIQASP
                     AWDPATHRSLLGRMLQLDPSLTERKKAAPAPTRESVRWTSWRSLAERQFQYKKLIEVD
                     LLKNSRDIAIARSYGDLRENFEYQAAKDFQRQLLQRQAEIQQELQLVKGTDFADAPHE
                     TAGPGTRVALCLADNTHIVYTILGEWDRDEALNIISSRSRLALCLTGRTAGDSVRIPG
                     PSGEETARIEAVLPLDEPIREWIRTPPPAMS"
BASE COUNT         1244 a         1905 c         2232 g         1317 t
ORIGIN      
        1 cccgctgaac gtcaacaagt aagctttttc gattcaacga aaacccccac aggcaaacgt
       61 tttcggtgga tcgggaaagc gtaacatcct ctgaactaaa acgttgaaag tgttgcaata
      121 gccggtagcc tgtgcgataa aaaagccgta agatgattgt cgagccgaag ggagggatga
      181 tgtatttcat cgggccgcat gtgtcgatcg ggggcggggt ggcgaatgcg ccgaaaaacg
      241 cgaaagcgct gggcgcgacc ggtttcggca tgttcgtcaa aaaccagcgg cagtggacgg
      301 ccgcgccgta tgccgcagcg gatatcgagg cgtttcggcg gcagatggcc gcagacggct
      361 acacggcggc gcaggtgatg ccgcacgccg gatatctcat caaccttgcg aatccggatg
      421 agacggccca tgcgaaatcc atgactgcgc tattggatga gttgcgccgt tgcatggcgc
      481 tcggacttga taaactcaac cttcacccgg gcagccatct gcgtctgatc acgcctcagg
      541 cggcctgcga acgcgtggca cagtcaatca accgggcgct gaatcagacg gagggcgtga
      601 ccctcgtgat cgagaatacg gcggggagcg gcgggaatct cggggcggtg ttcgaggaga
      661 tccgcacgat aatcgagggg gttgacgaca aatcccgcgt gggcgtctgc cttgacacca
      721 tgcatgcgtt cgcggccgga ttcgatatcc gcacgcgcga cgggttcctc aagaccatgg
      781 agcatttcga tcagacggtg ggcatggcgt atctgcgggg cctgcacctg aacgattcca
      841 aggtcgcgct cggttcgcgt gtggaccggc atgcgtcgct cggcgcgggg ctgctcggtc
      901 tcgatgtctt ctcctgcatc ttgacggatg cgcgctttga gaacatgccc ctcgtgctgg
      961 agacgccgga cgagacgatc tgggaacagg agatccggca gttgatggat atggcgggct
     1021 gacgcatccg gaaaggcgaa aggccgcata aacaggtggc gagaccccgc gcctattcga
     1081 acgccaccaa atttctttcg cgcaggctga cgtatccggg cgagacggcg gagggtttgc
     1141 ccacaatcag gtgatcgacc acccggatgc ctaaaatgcg cgccgcttca accagccgcc
     1201 gcgtgacttc aatgtcctct ttggagggga tcggatcgcc cgacggatgg ttgtgggcca
     1261 gaatcatgga ggccgcgctg tagcgtacgg ctttgttgaa gacctcacgc gggtggaccg
     1321 gactggagtc caacaggccg cgggacgttt cgatcggctg gccgatcaac cgactcttgg
     1381 tgtccaataa cagagtccaa aagatctcct gctgcagggt gcgcgtgagc gggagcagta
     1441 cccggtaggc cgcctccggg tcacggatca cgggcgtgtc atggcgcgga ccctgttggg
     1501 cggcacggcg tccgagttcg agcgcggccg agagttcaag agccttgacc ttcccgaggc
     1561 ccttgatccg gttgtgcaac agctcttcga aggtggtttt cgaaagcgcg gcgaggccac
     1621 cgtaccggag cagaatctcg cgggcgagtt cggtcacgtt cttgccggga agaccggagc
     1681 gtaacagaat cgccaaaagc agttcgtccg gcaggttttc cgcgccacgc cgctggagtt
     1741 cctcgcgtgg gcgcatctct accggcaggt cgcgcatcgg cggcgccgca tcgggataga
     1801 cggtacgttg gtcagaaggg caggggggtt gctccatgtt gcctccatgc tttgagaatc
     1861 cgttccgcga tccgctcggg cgggtcgcgc tcgtcggtat cgatccaaac cgcttgtgtc
     1921 tggtggcgga accaggtttc ctgtcgttta gcgagctgcc gcgtacggat cacgatccgt
     1981 tcaaaggcct cggcctgtga gagcgtgccg tccagaaggg cgcaactttc ggcgtatccg
     2041 atcgcctgcg ccgcgctggc ggaccagact gggtaatggc ggcggagcga gcggacttcc
     2101 tccacgagac cctgcgcgaa catggcctca acccgcgttc ggatacgccg gtggagctgc
     2161 tcgcgcggca tgcgcagaac cgtcaacggc gcgtcgggtg cgggctgttt ccagttttgc
     2221 ggcaggctgc ggaggctctc gagttgttcg atcgcgcgga tcaggcggcg cggattggag
     2281 gggtcggaga gggaggccag cgcctggggg tccagagcgg cgagagcctc gcgcagggct
     2341 ccgactcctt ccgtttccaa gcgcgcctgc caatgagcgc ggcgcgcggg gtcggccatc
     2401 gcgctgtcca atccgcgggt gagggctttg aagtagaggc ctgtgcctcc caccaccagc
     2461 agggagggga cagccttccc gtcggagtca gcggcattcg tatgcgcgac ggcgcgggcc
     2521 gcctctcgca accaggcgcc ggtactgaag ggttgatccg gcgtcacaag gtccacgccg
     2581 aaataggtca cccgcgcacg ttctgccgcg gagggtttgg cggtgccgat atccatgccg
     2641 cgataaacca gcatggcgtc ggcggagagg atcgcgattt ccaatcgttc cgccaagagt
     2701 tgtgcggcgg cgctcttgcc tgtggccgtc gcgccggcca gtgtccaagc gagggtcatc
     2761 gggttgtcag tcccctgttt ccggttccgt catctggtcc cgcgccatat gtcggtcatg
     2821 gcgccactgc atgatgaggt agaggaagac gccgcagcag attgccgaat ccgccacatt
     2881 gaaggcgggg aaatgcgcgt cgccccaatg gaaatcgaga aaatcgatga catgccccag
     2941 acgcaaccgg tcaatgaggt taccgaccac accgccgaag agcagggaca tgatcaccgt
     3001 ccggccgcgt aatgcgccga agagcgcgcg gcgacgccag atcatgaaga tcagggtggc
     3061 gatcgaaaaa ccgatcagga aggcttggcg tccggcgagc attccccacg ccgcgccctg
     3121 attctccacg tagctgaagc tgaacaggcc ggggatcacc gacaccgatc tcaccgcctg
     3181 aagccggacg accgcccaat attttgaaat ctggtcggcc gccaccagca gggccgcgag
     3241 gaggagaacg cgcatctcaa aaccttatct gcgtccgcgg aaacggaact gtttttccat
     3301 ttcagattgg cagcggatgc agttcttggc gaacggcaac acggccagac ggagtttact
     3361 gatcaactcg ccgcaggtat cgcacacgcc gtaattcccc ttctcgatcg accgcaacgc
     3421 ctcatcgata ttcgtgatgg tttgcagttg gctgctgacc tgttccaggg tttgcaggcg
     3481 catgaaggcg ttggtcccgt cctcttccgg gttggtctcg tcggtgcgct gtagcgcggc
     3541 gtggcgcatg gcacccgact gtccggtgat gcgttcgcgc atggagagca ggtcgcgttt
     3601 gaatgcgttc aggtcgcgtt tgctgaagtg ggggctaccg cgcacgggct tcttggctac
     3661 gagcttataa gacggcggtc cttttttcac gggcgcttcg gttgaggtgc cccgttttac
     3721 agcggacttg cggacaacgg ttcgggattg ggttatgggg ggggtcttcg cggtccgcgc
     3781 aggtttcgcc tgcgcagccg gtttccgtgc cgcgggcttt ttcgctatgg ccgcagtggc
     3841 gcgtttggcc ggggcggcgc gtttggccgg ggcggcgcgt ttggccgcag tggcgcgttt
     3901 ggccggggcg gcgcgtttgg ccggggcggc acgtttggcc gtggctttct gcacgggctt
     3961 gggggggctc ttcttggtgg agccggtctt ggttttacgg gtagttgcca tcggacacct
     4021 cgctctctcg ataatccgtt ttgcagtgaa gaatggcgca ttatacaggg caagacgcgg
     4081 gatcacaagc cccgaatccg accgggcgga ttctcttgat cccaaaggct ccccgggtcg
     4141 aatcgcgttt caagacatcg cggggggagg tgtgcggatc cattcgcgga tgggttcgtc
     4201 caagggcaga accgcctcga tccgggcagt ttcctcgccg gacggtccgg gtatacgcac
     4261 cgagtcgcct gcggttcgcc ccgtgaggca gagggcgaga cgcgatcggc tggaaatgat
     4321 gttcaatgcc tcatcacggt cccactctcc gaggatcgtg tacacgatat gcgtattgtc
     4381 ggccagacat agcgccacgc gtgttccggg acctgccgtt tcgtgcggcg cgtcggcgaa
     4441 gtcggtccct ttgacgagtt ggagttcctg ctgtatttca gcctgacgct gtaacagttg
     4501 ccgctgaaag tccttggcgg cctgatactc gaaattctca cgcaagtcgc cgtaactccg
     4561 ggcgatggcg atgtcccggc tgttcttcag gaggtcgacc tcgatcagtt ttttgtactg
     4621 gaactgccgt tcagccagag agcgccagga ggtccagcgc acactctccc gcgttggggc
     4681 cggggcggct tttttgcgtt cggtcaggga cggatcaagt tgcagcatgc gtcccaacag
     4741 ggaacggtgt gttgcgggat cccaggcggg cgaggcctga atgcgctcaa agatccgttg
     4801 gcgttggggc atgtccagtt ccgcgaacac cgattccagc catgaggttt ggccgaaaag
     4861 gcgtttgagt tggttctgca tgcgcagtgc ctcgccggag aggcggcttt cgacaatctg
     4921 gatcgcatga tccagcagtt cgtgcagcgg cggcagccgc cagccgggcg tgtcgcggtt
     4981 gcggaaggcc caataaacga gcgtgggcgg cgccttgggc tgagtcagca gtctgcggca
     5041 cgcctcctcc gcctcggccg aagtgttcag ccggttcaac acctcgttga gcagggagaa
     5101 cggcatctgc ggcagggcgt ccagcaatcg ttgcgcggcg gcggagcctt ccgcgagcag
     5161 gaaggtcgcg aggtctgcgg tgtctttgac ggtcagggct tcggatgcgc ggaggaaacg
     5221 gtgctcttcc cagaggtgcg cgcgcaactg atcggtcggc ggggtcagaa atccgaagcg
     5281 cgagagcatg acggccaggc gggcgtacag tgccgcatcg gactgatgcg cggcgccgag
     5341 ggcgaaatgg agccgctctt ccagaatggc gcgctgggcc gcatctagat tttcgatccg
     5401 gccggcagtc tcaagctcga tcgcctgcgt aaggaggcgc tccgggtttt tggtcgcgct
     5461 gaaggtggcg aaccaggcat cgccgtacga ctcggcttcg gtcagcagac ggagcggttc
     5521 gcttttttta gcggggaaga cgaccagcgg atcctgtttc agcgccttgc gcgccgcgtc
     5581 ccaaaaccct ttccactcgt ccggggcgag aatcttttcc tggacgagca ggtcttcgat
     5641 cctgaccgcg gtcaggtcgc cgtaactccg caaggtcagc cggagtagtt cgccgggctg
     5701 ctctcttacg atctgcgcga aacctccggg atcgttatgg cgcacggtga gcagatgatc
     5761 ttgcggggcg ggcacgaggc tatcgcaggc tgtggcaaag gggatttcat ggcgcggttt
     5821 ccgggtaaag tccacgatga aacgtttata gaagctgtcc gcctcctgaa tgaccccgaa
     5881 cccccacgct ggatcgatga cctgtgtgcc cggttccaac gcgagcaggg tgccgaggcg
     5941 tttcaacgct tcgtaaagcg gcttctctcc gaactgggct acatccagca tcacatcggt
     6001 gaggcggtcc gggcaggtct gcttgaacag gcgcaggatc gcatcgggtg cgagggcgca
     6061 ggaaagcggc tccacctgat ccatgacagt caggaagaga ccgcggaaat ccgttgcgtc
     6121 gaccagttcc tcgatgagca gcatggtcca ttcttcagct ttacccgcgt ctgtggtggt
     6181 cgtgatcgcc tgaagcaggg gcaacagatc cgtaaccggg agagggcgga tttcaagcag
     6241 ggaaaagaat cgtgtctcga gttcttcgaa agtcattgcc gcatccatca taacggaacc
     6301 ttctcttccg tgcgtttaca gtttggtctc cggaatcttc ggcttggatg tttccgcccc
     6361 tttcgcgccg ctcgccgcga gaaacgcgac acccgcgtcc atgaaaaccc ctcgtgaaaa
     6421 attcatctca ttctcctctc tttatacacc agagtgataa caaattcgga acgggagagg
     6481 caagcgtcgg cttgtcagat gcggccaaag cccgcagtag aagacaggag aggagaggtg
     6541 ggggattgga acgggaagga cgcgcaattc tcagttcgta ccctgaaaca catgtgaaag
     6601 caaaaaggga ggggtggaaa gtcgggagca aggaaaaact gcagcggcag gggcttggtg
     6661 cttgacttcg tggactgcgt caggcatcat aaaattag
//
LOCUS       DAOSLE010000142         6617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10534_length_6617_cov_7.040232, whole genome shotgun sequence.
ACCESSION   DAOSLE010000142 DAOSLE010000000
VERSION     DAOSLE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10534_length_6617_cov_7.040232"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..3267)
                     /locus_tag="PK576_08885"
     CDS             complement(115..3267)
                     /locus_tag="PK576_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61746.1"
                     /translation="MKMLKSLVALAGVSGMALGAEFHVAKNGNDTNDGSVAKPFATVE
                     RAVTALRAVRASTPQEPVRVVIGAGTFELRAPILLTDRDTTGPVAFVGKGATQTILSG
                     GREIAPALWRIGGDGRSWAVTLPEVSNGTWRVQQAYAGGRRVLRPSLPRNNYFVAEGK
                     PADVEKPVPNRLIAEAGDLDTCRDDLTNGVEFVAMHGWNITRARVKAFDRVTREVTLA
                     VGRPGYLRGFDSNSYYRLENVKSAYGEVEGEWYCEPQTGLLSYLPLPNETPVNAPFVV
                     PVLTNLVTVQGDRATGRKATNVSFQSLGFAYVSEPLQEPGTVYHQTSLEAGTAIRVDF
                     AENVSIDACAVRHTGGWAVKIEGGCVGCAVTGSTFFDLGAGGVALGVKGARRGNEERH
                     THDCRVERCLIEGGGRVQAGAVGICVVLAGGNRIAHNTIRDFYYSGISVGYDWSDKND
                     AASGNIVEWNHIYDIGQRVLSDLGGIYTLGRQPGTILRCNLIHDIRTARYGAQGIYMD
                     QGSAEILACSNIITRADEAAFSVASGACGGIRFENNIFLFPRRYVIWTFNDKQKGHDV
                     TVARNIFMWDDKLRDMFPEGGPAKEWRFSSNVWWQAAWPERIYFRKPGGTNAFVLADA
                     PAGSRWKQWHKLAMPSPDVAAWLAAAPGDTLEDPRLGAAYGDEKHLKPWPDSPAVVRG
                     FVPFDLSAGVGHEPQAQPYARKRPLVSATRTFPAAPLSRKNKAALAAETAKGELHMTV
                     FNQRYILGGAGMGVAFRTPAGKTFFFDMGNGDDTVVAHNCGRDTIDPWLYVNDIRKID
                     GVVMSHAHGDHFGGLLALWNHYPVDYFWDNDYRMPGQPVNAQNFGELGAVRRLRAQLK
                     SVNPHLVCRGVRQFDKLDWDPVLDVTVITPPKAGIPYLVNPNRPKNDGPEHHLINANA
                     LGIRVEYGKVSYQIVGDIQADYEKAFLLPTLPDKFKTCDILMPCSHGIHATDEAANVF
                     RPKVAIGTVYADGAADGWSRTWAFTLPIWNVYRKVGAEVYVSGVDGDVTASSDGKTWR
                     VTTEFGANRRVNPFSRPARR"
     gene            complement(3542..5356)
                     /locus_tag="PK576_08890"
     CDS             complement(3542..5356)
                     /locus_tag="PK576_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61747.1"
                     /translation="MIEDVYEPLALYRDRFKAEHADNTAAFFERLVERSGVDENANAA
                     TVAEIRTLDAQLAAADSSRSNWKTLRMLLVIIVVAGVIGLILFILPIFSPDIEPPIRV
                     GGLWAAACAAVAAGGIALIAAKLNPALRLLDERLRLLTEYREAKMTEALAQLAPLNRL
                     YDWGMIAKLIQQTVPRLALDPYFSRARLDELHRSFGWDDSFNRDRSVLFAQSGEINGN
                     PFVLAETLDFHMGAKTYHGSITISWQERESYTDSEGRTRTRWVTRTETLHASVDKPAP
                     EYSREKFLIYGNEAAPDLTFSRNPSNLSNAGDGFFSRWRMRRAVAKLEAFSRNLDDEY
                     GYTIVSNRQFAALFHAIDRNDEVQFRLLFTPLAQQQMLNLLRDRTVGFGDNFTFVKDR
                     MINLVRPAHLATTDITAAPSLFHHYDLAAAREFFNTYSNAYFRALYFALAPVLTIPLY
                     QQHRSHADIYASVLGKTASFWEHEAIANFHGQHAFGHHEAITENLLKTQAVRTDDGYT
                     DVVVTAHGFRGEHRVDYVSKFGGDGRWHKVPVKWIEYIPVQRTSTLTIRETDGLTLQD
                     YEEQSQTSPDWQTFFRKYQIEPKHVSFRRSIVSFVSPS"
     gene            complement(5353..6015)
                     /locus_tag="PK576_08895"
     CDS             complement(5353..6015)
                     /locus_tag="PK576_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOE61748.1"
                     /translation="MANELDEMNAPVNQQGRDINVIEKQIPVKVGFGSTLFEIMLWVL
                     GILPGLIFLFMKIGARNYFQKLQQKIQADASQIDNFLEQRVQILQNVAPLVEKAIDLD
                     KDVMKTVSAFRGGAHADNERNVVSGHLDRAFGRLFPQVEAYPDLKAHAAIADAMRQNS
                     YLQKEITAARALYNDTVNIWNSDIFSWPTKMIVAARAGYTTRIPFTASAETKAQARST
                     FF"
     gene            complement(6436..6519)
                     /locus_tag="PK576_08900"
     tRNA            complement(6436..6519)
                     /locus_tag="PK576_08900"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6484..6486),aa:Leu,seq:gag)
BASE COUNT         1395 a         1937 c         1917 g         1368 t
ORIGIN      
        1 tttggttcgg gaccaaaagg tcgagggttc aaatccctcc gctccgacca atttttcaga
       61 gtgcaaatcc acgaaaacgc tcatgttttc gcggtttttt gtttggggaa gtcatcaacg
      121 ccgtgccggg cgggagaagg gattcacgcg gcggttcgct ccgaactcag tcgtcacgcg
      181 ccatgttttc ccgtcagaag acgccgtcac gtcgccgtcg actcccgaga cgtagacctc
      241 tgcgcccact tttctgtaga cgttccagat gggcagggtg aacgcccatg tgcggctcca
      301 cccgtccgcg gcaccgtcgg cgtacaccgt gccgatggcc actttcgggc ggaacacgtt
      361 cgccgcctcg tccgtcgcgt ggatgccgtg tgaacacggc ataaggatgt cacacgtctt
      421 gaacttatct ggcagcgtgg gcagaaggaa tgccttttca tagtcagcct gaatgtcgcc
      481 gacgatctgg taggagacct tgccgtactc cacgcggata cccagcgcgt tcgcattgat
      541 aagatggtgc tcgggcccgt cgttcttagg gcggttcgga ttgaccaagt acggaatgcc
      601 ggccttcggc ggcgtgatca cggtcacgtc cagcacagga tcccaatcca gtttgtcgaa
      661 ttggcgcaca ccgcggcaga caagatgggg gttgacggac ttgagttgtg cgcgcaaacg
      721 ccgtacggca ccgagttcgc cgaaattctg cgcgttcacg ggctgtccgg gcatgcggta
      781 gtcgttgtcc cagaaataat ccacgggata gtggttccac aaggcaagca gcccgccgaa
      841 gtggtcgccg tgggcatgac tcatcaccac gccgtcgatt ttacggatgt cgttcacgta
      901 gagccaggga tcgatcgtgt cgcggccgca gttgtgagcc accacggtgt catcgccgtt
      961 acccatgtcg aagaagaacg ttttgccggc aggtgtgcgg aacgccacgc ccatgccagc
     1021 gccgccgagg atatagcgct ggttgaacac cgtcatgtgc agttcgccct tggccgtttc
     1081 ggcggcaaga gccgccttgt ttttgcgcga gagcggcgcg gcggggaacg tgcgcgtggc
     1141 cgagaccaag ggacgtttcc gagcgtaggg ctgcgcctgt ggctcgtgcc cgacacctgc
     1201 cgaaagatcg aatggcacga agccgcgcac gacggcaggg gaatccggcc agggcttcaa
     1261 gtgcttttca tcgccatagg cggcaccgag gcgcgggtct tccagcgtgt cgcccggagc
     1321 ggcggcgagc cacgccgcga catccggact gggcatggcg agtttgtgcc actgtttcca
     1381 gcgcgaacca gcgggcgcgt cggcgaggac aaacgcgttc gtgccgccgg gtttacgaaa
     1441 ataaatgcgc tcgggccaag ccgcctgcca ccacacgttg ctgctgaaac gccactcttt
     1501 ggcaggacct ccctccggga acatgtcccg cagtttgtca tcccacatga aaatgtttcg
     1561 tgccactgtc acgtcgtgcc ccttctgctt gtcattgaat gtccaaatga catagcggcg
     1621 cggaaagagg aagatgttgt tctcgaatcg gatgccgccg caagcgccag acgccacgga
     1681 gaacgccgct tcgtccgcac gcgtaatgat gttgctgcat gcgaggattt ccgcgctgcc
     1741 ttgatccatg tagataccct gcgcgccata gcgggccgta cgaatgtcat gaataaggtt
     1801 acaacgcagg atcgtgcccg gctggcgtcc gagcgtgtag atgccgccga ggtcagataa
     1861 gacacgctgg ccgatgtcgt agatgtggtt ccactcaacg atgttgccgg acgccgcgtc
     1921 gttcttgtcg ctccagtcat atcccacgct gatgccgctg tagtagaaat cgcgaatcgt
     1981 gttgtgcgcg atccggtttc cgcctgcgag taccacgcag atccccacgg caccggcctg
     2041 gacgcgtccg ccgccctcta tcagacaccg ctccacgcgg caatcgtgcg tatggcgctc
     2101 ctcgttgcca cgccgcgcgc ccttcacgcc cagcgccacg ccccccgcgc cgaggtcgaa
     2161 gaacgttgac cccgtgaccg cgcaccccac gcagccgcct tcgatcttca cggcccagcc
     2221 gcccgtgtgg cgcacggcgc aagcgtcgat cgacacgttt tccgcaaaat cgacccggat
     2281 cgccgttccc gcctcaagcg atgtctggtg atacaccgtg ccgggctcct gcaagggttc
     2341 ggagacgtag gcgaaaccca acgactggaa cgacacgttc gttgccttgc gtcccgtcgc
     2401 gcggtcaccc tgcacggtca cgagattggt tagaacgggc acgacgaacg gggcgttcac
     2461 gggcgtttca ttcggcaacg gcagatagga gaggagtccc gtctgcggct cgcaatacca
     2521 ttcgccttcc acctcgccat acgcgctctt cacgttttca agacggtagt aggaattgga
     2581 atcaaagccg cgcagatagc ctggccgtcc caccgcgagc gtcacctcgc gcgtgacgcg
     2641 gtcgaacgcc ttcacgcgcg cccgcgtgat gttccagcca tgcatcgcca cgaactcgac
     2701 gccattcgtg agatcgtccc ggcacgtatc caaatcgccc gcttccgcga tgagacggtt
     2761 cgggacaggc ttctcgacat cggcgggttt cccttctgcg acgaagtagt tgttgcgcgg
     2821 cagggatgga cgcaacacgc ggcgtccccc tgcatacgcc tgctgcacgc gccatgtgcc
     2881 attcgagacc tcgggcaacg tcacggccca actgcgccca tcccccccga tcctccagag
     2941 cgcaggcgcg atctcccgtc cgcccgaaag gatcgtttgc gttgcgccct ttccgacaaa
     3001 cgcgacaggt cccgtcgtat cccggtctgt caacaggatc ggcgcacgga gttcaaacgt
     3061 gcccgcaccg atcacgacac gcacaggctc ctgcggcgtg gaggcccgga cagcacgcag
     3121 cgccgtcacg gcacgttcga ccgtggcaaa aggcttcgcc accgacccgt cgttcgtatc
     3181 gtttccattc ttggccacat ggaattcggc acccaaggcc attcccgaca cccccgccag
     3241 ggccaccaga ctcttcagca ttttcatcgc ataacctttc tctctcaaga tatctagaca
     3301 atcaatacgt ccacattccg ttcatacggt ctgttaatgc agcatcatac cataacccgc
     3361 cccacgacac cgaatccaat tttaagtttt gatgtttttc atatctttgg atcatcgtca
     3421 ttcatgtgga caggttcaat catgattcac gcaatccaaa cccatacgcg ggttttcaat
     3481 gtgcaagaca atacgttacc cccccggaac gatccacggg aaagatggca agaggtacgc
     3541 cttacgaagg ggacacgaac gaaacgatag accggcggaa cgatacgtgc ttcggctcga
     3601 tttgatattt gcggaaaaag gtttgccagt ccggcgaggt ttgtgactgc tcttcgtagt
     3661 cttgtaacgt aagtccgtca gtttcccgaa tcgtcaatgt tgaggtacgc tgtacaggga
     3721 tatattcgat ccatttgacg gggactttat gccacctacc gtctccaccg aactttgaga
     3781 catagtcaac tcggtgctct ccccggaaac cgtgcgcggt aacgaccacg tccgtgtaac
     3841 cgtcatccgt acggacagct tgcgttttga gcaggttttc ggtgatggct tcgtgatgcc
     3901 cgaaggcatg ctgcccgtgg aagttggcga tggcctcgtg ttcccagaac gaggcggtct
     3961 tgcctaacac gctcgcatag atgtcggcat gggaacgatg ttgctggtaa aggggaatgg
     4021 ttagaaccgg agcgagggca aaatacaggg cgcggaaata ggcattgcta taggtattga
     4081 aaaactcgcg ggcggcagcg aggtcgtagt gatggaaaag cgagggggca gccgtaatgt
     4141 cggtggtcgc gaggtgtgcg ggtctgacaa ggttgatcat gcggtccttg acaaaggtga
     4201 aattgtcgcc gaagccgacg gtcctgtcgc gcaagaggtt aagcatctgc tgctgggcca
     4261 ggggggtaaa gagaaggcga aactgaactt cgtcgttgcg gtcgatggca tggaaaagcg
     4321 cggcaaactg ccggttggac acaatggtgt acccatactc atcgtcgagg ttgcgggaga
     4381 acgcttcgag tttagcgacg gcgcgcctca tgcgccacct gctaaaaaac ccatcgccag
     4441 catttgagag gttggaggga ttgcggctga aggtgaggtc cggagcggcc tcgttgccgt
     4501 agatgaggaa tttctcgcgg gagtattccg gcgcaggctt gtcgacggat gcgtgaagcg
     4561 tctcggtgcg cgtgacccag cgcgtccggg tgcggccttc ggaatccgtg tagctttcgc
     4621 gttcctgcca agagatcgtg atcgagccat ggtaggtctt ggcacccata tggaagtcga
     4681 gcgtctcggc cagaacaaag gggttaccgt tgatttcccc gctctgagcg aacagtaccg
     4741 accgatcacg gttgaaactg tcatcccagc cgaaagaacg atgtaactcg tcgagtcgcg
     4801 cgcgggagaa gtatgggtca agtgcgagac gcgggacagt ctgctggatg agcttggcga
     4861 tcatgcccca gtcataaagc cggttgagcg gggcaagctg cgctagcgcc tcggtcattt
     4921 tcgcctcgcg gtattccgtg agcaaccgga ggcgttcgtc cagtaagcgg agggccggat
     4981 tcagtttggc agcgataagc gcgataccgc ccgccgcaac ggcagcgcaa gctgccgccc
     5041 atagaccgcc gacgcgtatc ggaggctcaa tgtccggcga gaagatcggc aaaatgaaga
     5101 gtataagacc gatgaccccc gcaacgacga tgatgacaag caacatccgc agtgtcttcc
     5161 agttagaacg gctcgaatcc gcggcagcga gttgagcatc gagcgtacgt atttccgcca
     5221 cggttgcagc gttggcgttc tcgtccacac cggaacgctc gaccagcctc tcgaaaaagg
     5281 cggctgtatt atccgcgtgc tccgccttga agcggtcgcg atagagggcc aagggttcgt
     5341 agacgtcctc gatcaaaaga acgtgctccg cgcctgcgcc tttgtttcag ccgaggctgt
     5401 gaacgggata cgcgtggtgt agcccgcgcg ggcggccacg atcattttcg ttggccaaga
     5461 gaagatgtcc gaattccaga tgttgaccgt gtcgttgtac agcgcgcgcg cagccgtgat
     5521 ctctttctgc agatagctgt tctggcgcat ggcatcagca atggcggcat gagctttaag
     5581 atcgggataa gcttcgacct gcgggaaaag acgaccgaag gcgcggtcaa gatgccccga
     5641 cacgacgttg cgctcgttat cagcgtgtgc gccgccccga aatgcggaaa cggttttcat
     5701 cacatcctta tcgagatcaa tggctttctc gaccagcggt gcgacgttct gcaaaatctg
     5761 cacgcgctgc tcaaggaagt tatcgatctg cgaggcatcg gcttggattt tctgctgaag
     5821 cttttggaaa taattcctcg cgcctatttt catgaagaga aaaatgaggc cgggcaggat
     5881 gccgagcacc cacagcataa tctcgaaaag cgtcgagccg aagcccacct tcacggggat
     5941 ctgcttttcg atcacattga tatcccgccc ctgctgattg acaggcgcat tcatttcatc
     6001 cagttcattc gccattctgt aaactcccaa tcaatcaaaa caagcatcct gccacacgat
     6061 tactgaacga tagcatacgc cctaccccat ttcaagtcgt ctgacgggac ttacagacaa
     6121 aacgcttact ctgccaaacc tttgtgtgtg actaaattgg gttgcggact ccgcccgaat
     6181 tcgttgattt acagagcgca taagcgttta ccaatagaag aacgcagaag agcataggga
     6241 caacataggc aacattgcct gtagtcaacc agattaccgt tgggccttcc gttcctccaa
     6301 tgattacggc atctttatca atggaacctg aaggccatgt cgtccatgcc aatgccatca
     6361 gacatatgac gatgattgca gttactacat taatgagcgc caaaactttt ttcattacat
     6421 tgcgcagctt taaagtggtg ctcggggggg gactcgaacc cccatgcctc gcggcacatg
     6481 cccctcaaac atgcgtgtct gccattccac cacccgagcg gggaaacggt ggtactgtaa
     6541 cggattcgat ggcggaaagg caaggggaaa ccgcaggact gctgtttctt gtgtttcatg
     6601 gtacggaacc ggaaacc
//
LOCUS       DAOSLE010000143         6483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10820_length_6483_cov_8.392190, whole genome shotgun sequence.
ACCESSION   DAOSLE010000143 DAOSLE010000000
VERSION     DAOSLE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10820_length_6483_cov_8.392190"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /gene="rpoN"
                     /locus_tag="PK576_08905"
     CDS             <1..865
                     /gene="rpoN"
                     /locus_tag="PK576_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE61749.1"
                     /translation="PGVGARDLRECLLIQLRERGYEEDPAYEIVDKCFDELMHKRTKE
                     IMRITRLSKEDVAEALEVISTLNPKPASVAYGSSERPVTPDLIVERVDGEYVVSLNDR
                     SIPALRISQNYRELLAGSGTEETKKYIVDKLNSAKWLIKSIEQRRTTMLKVMNAIVKH
                     QRAFFDYGLDHLKPMVLQEIADEIKMHVSTVSRVSNGKYVQTPHGVFELKFFFDGKLG
                     VSDGEDMASKVVKEKVKQMIDAEDKQNPLSDQAIADRLTRESGIEIARRTVAKYRDQL
                     RIPSARMRKEV"
     gene            867..3269
                     /gene="sppA"
                     /locus_tag="PK576_08910"
     CDS             867..3269
                     /gene="sppA"
                     /locus_tag="PK576_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOE61750.1"
                     /translation="MSFDRFWSPVVLSMLFSGSLFASTPFVTSTDRFRSQLGIVENPA
                     LSDEASAVWVNPAGLGVRKSATMFSSVAMRNNPWYANLLFAGNGSGFGWQRTDAGSGQ
                     RVDRWRFGGSGGSSPYGVSFGAAVELSDPDGLKENLFWSGDLGVLARPVTWMSAGLVV
                     RQLGARRGYPWSVESGLALRPFGPNLSIFGGLAYCEDDPLSDPSHWHAGALANVGPGL
                     EAYGAINQNRTILVGVQMILGRGSIGGAGSRVSGGSLGSGWVIARSHADYRSNRLAMK
                     GRIAEIRLKGEIRDQTPGFSLFGNRGTTLSELVMQINRAAQARDVGGLYLRFDNLAIG
                     QGMAEELRDALVKFKANSGKPIVAYLPEASFREYFIASVADSIFLEPVGDLRLTGYGV
                     GQLYFRRALDKLGVEADFTRIGRYKSAAETFTDSTMSDATREQYEELLDDWYTRTVDG
                     IAVSRRLSADSVKALVNNAPYMAAEAVRVGLIDSAGHSDRAYESVETMVRSREGRVSG
                     KINLARRRLYDETWGPRPKLAVIFASGQIVNGTSGEDFFSGTQMMGAETIAKALKQAR
                     EDDAIKAVVFRIDSPGGLALGSDIIWREVQLLWETDKPVVVSVGDLAASGGYYIACRA
                     DTIISNPGAIVGSIGVFDGKMVVERLAHRLGIDVELLARGDNAAINSSLASRTPEQRR
                     RVAENVREVYDVFVNRVAAGRGMEAASVDSIGQGRIYTAANAVSIGLVDKLGGLDEAI
                     RTAARMARLRGEVELVTMPRHTNVLETVIQSSLQDAMGVSTRQSLAGGVYFFDPVAAS
                     LR"
     gene            3347..6463
                     /locus_tag="PK576_08915"
     CDS             3347..6463
                     /locus_tag="PK576_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOE61751.1"
                     /translation="MKDVVKGTTGFAAPAAKSLLACAIVLCLMCSHAHAQVGEFGRNK
                     PTSATGNWHYVQTPHFDIYFYEGMDAIARIATTIAEEAYSQISASFDYRLRNRVPIVL
                     YRSQGQFSQTHITPSLIGEGVGGFTEFLKNRVAVPFTGSYPQFRHVLHHELVHAIVHD
                     MLYGRDILTGVQQSDIPLWFIEGLAEYESSPWTSESDMWMRDAVINDYLQLDGYFAYK
                     GGQSIMYYIAEVYGPDKVAQILNRLRSFSRFGPTVQAVLGVELEDFGKRWEKDLKKVY
                     WPEIVDRDEPEDIGQSLTDHTKDNSYFNRNPSFSPFGDKIAYLTGRDYYTSIYVMSAI
                     DGKVLWKVVTGETSGSFEEMHWQRAGIAWSPDAQRIAFAAKATTGDRLYIVDARNGRR
                     LDAFDFGFDGMFSPDWSPDGTTIVFVGVRDGKSDLYTLRLSDRSIDRLTDDYFDETDP
                     KWSPDGRRILFASDRAPDVSDVSAQLLYQTSDVYVMDVETRAMRRLTADPADDLSPAW
                     GPEGKHIAFVSDRNGIFNLYVAALDSGEGSIDSATRPLTNILTGAFAPSWNRRGDKIA
                     FSGFNKGGWDVFVIRNPLDSLREEPLRPAYFLREGKISYSSRHRHLATSTQSATSPDS
                     TAGFSIGPRTSPFSSPALDILAFTRADSARGGFVPKPYRSQLQVDNVSGQANYSTLGG
                     VGGQLYVTASDLMGFHQLQFNTSLYRDLRDSDYGVFYQYLKRRTDYAAYFQQQRTWYP
                     FQSVDYYGGIVESGLVESREISTGVYASRPTSQFRRFEAGIDYVNAKQSVLYDYLSYG
                     TGIAASQEATIEALLTRVAFVHDNALWNMFGPVVGQRWRMTVFAAPPRANTAPFGVVT
                     ADVRRYWRVSKNTSFALRGSGGRSFATHAQNAPLFYVGGLPYWINYDYENVQSSQLVN
                     DAFSQFVFPLRGTNYYELRGRSFAILNAEYRFPLIYFLATGPLNFVLSNVGGVVFLDV
                     GRTWEDPTQPSDPNIARHAQGPLGGYGYGMRINLGVLVLRMDVAWRTDLRETIGSARY
                     YWSFGYDF"
BASE COUNT         1369 a         1720 c         2015 g         1379 t
ORIGIN      
        1 gccgggtgtc ggtgcgcgcg atcttcggga gtgccttctc atccagctgc gggaacgcgg
       61 ttatgaagag gatccggcct acgaaatagt ggacaagtgc ttcgacgaac tgatgcacaa
      121 gcggacgaaa gagatcatgc gcatcacgcg cctgagcaaa gaagacgtag cggaggctct
      181 cgaagtaata tccacgctga acccgaagcc tgcttccgtc gcctacggtt cttccgaacg
      241 cccggtaaca ccagatctca ttgtcgagcg tgtggacggg gaatatgtgg tttctctgaa
      301 cgaccgatcc ataccggcac tcaggatcag ccagaactat cgagagctcc ttgcgggcag
      361 tggtaccgaa gaaacgaaga aatacatcgt ggacaagttg aattcggcga aatggctcat
      421 caaatccatc gaacaacgcc gtacgaccat gctcaaagtc atgaacgcca tcgtcaaaca
      481 ccagcgagcg tttttcgact acgggcttga ccatctgaag cccatggttc tgcaggaaat
      541 cgccgacgag atcaagatgc acgtttccac ggtgagccgc gtatcgaacg gcaagtacgt
      601 tcagacgccg catggcgtgt ttgaactgaa gttctttttc gatggcaaac tcggtgtctc
      661 agatggcgaa gatatggcct cgaaagtggt caaggaaaaa gtgaagcaaa tgatcgacgc
      721 cgaggacaag cagaacccgc tcagcgacca ggcgattgcc gaccggctga cccgcgaaag
      781 tggtatcgaa attgccagga gaaccgtggc gaaataccgt gaccaactcc gcatcccatc
      841 cgcacgcatg cggaaggagg tttgatttgt cgttcgacag gttctggtcg cccgttgttt
      901 tgtcaatgct gttctctggg agtcttttcg ccagcacacc gtttgtaacc tccactgacc
      961 gttttcgaag ccagctaggg attgttgaga accccgctct ttccgatgaa gcgagcgcgg
     1021 tgtgggtgaa tcccgcaggg cttggtgtgc gcaaatcagc gacaatgttt tcttccgttg
     1081 caatgagaaa caacccctgg tacgccaacc tgctttttgc aggcaatgga agcggtttcg
     1141 gctggcagcg aacggatgcc ggcagcggcc aacgagttga ccgctggcgc ttcggaggaa
     1201 gcggcggaag ctccccgtac ggcgtgtcat tcggcgctgc ggtcgaactg agtgatccgg
     1261 acggtttgaa agagaatctt ttctggtcag gggacctcgg tgttctggcg aggcccgtta
     1321 cgtggatgtc cgccggcctt gtcgtgcgac aactcggcgc tcggcgtggt tatccgtgga
     1381 gcgtggaatc gggccttgct ctgcgcccgt tcggaccgaa cctgagcatt tttggcggtc
     1441 tggcgtactg tgaagacgac ccattgagtg atccttcgca ctggcatgcc ggtgcactgg
     1501 cgaacgtggg gcctggtctt gaggcgtacg gggcgatcaa tcagaacagg acgattctgg
     1561 tcggcgtcca gatgattctc ggaagaggca gcatcggcgg agcagggagt cgcgtgagtg
     1621 gcggaagcct cggttcaggc tgggtaattg ctcgctccca tgcggattac cggtctaacc
     1681 ggctggcgat gaaaggcagg atcgcagaga ttcggctgaa aggtgagatt cgcgatcaga
     1741 cgccgggatt cagcctgttt ggcaaccggg gcaccacgct ttcggaactc gtcatgcaga
     1801 tcaatcgggc ggcgcaggcg cgcgatgttg gtgggctgta ccttcgtttt gacaacctgg
     1861 cgatcgggca gggaatggcg gaggaattgc gtgatgcgct ggtcaagttc aaggcgaact
     1921 ccggcaagcc cattgttgcg tatctgccgg aggcgagttt ccgggaatac ttcattgcct
     1981 cggttgcgga cagcatcttc ctggagccgg tgggcgacct ccgtctgacc gggtacggcg
     2041 tggggcaatt gtacttccgc agggcgttgg acaaactcgg cgtcgaagct gatttcaccc
     2101 ggataggccg ttacaagtcg gccgctgaga cgttcacgga cagcaccatg tcggatgcca
     2161 cccgggagca gtacgaggaa ctcctcgacg attggtacac gagaacggta gatggaatcg
     2221 cagtgtctcg gcgcctcagc gctgacagcg tgaaggctct ggtcaataac gctccctaca
     2281 tggctgcaga agcagttcgt gtgggcctga tcgattccgc aggtcacagt gatcgagcct
     2341 atgaatccgt ggaaaccatg gtaaggtcga gagaggggag agtatcgggc aaaataaacc
     2401 ttgcgcgtcg tcgtttatat gatgagacgt ggggcccgcg tccgaaactg gcggttatct
     2461 tcgcgtccgg gcagattgtg aatggaacga gcggggagga tttcttttcg ggaacccaga
     2521 tgatgggtgc cgagacaatt gcgaaggcgc tcaaacaggc tcgggaggac gatgccatca
     2581 aggcggtcgt gttccggatc gattcccccg ggggactggc tctgggcagc gacatcatat
     2641 ggcgggaggt acagcttttg tgggaaaccg acaagcctgt cgtcgtaagt gtgggcgatc
     2701 tggctgcttc gggcgggtat tacatcgcat gtcgcgcaga cacgatcatt tcgaatccgg
     2761 gcgccatcgt tggcagcata ggggtatttg atggcaaaat ggttgttgaa agactggcgc
     2821 accgactcgg aatcgatgtg gaacttctcg cgaggggaga caacgcggcg attaattctt
     2881 cactcgcatc gcgaacaccg gagcagcgaa ggcgagtggc cgaaaacgtt cgtgaagtgt
     2941 acgacgtgtt tgtgaacagg gtcgccgcgg gccgcgggat ggaagccgcc tcggtggatt
     3001 ccatcggtca gggccggatt tacactgcag cgaatgccgt cagcataggc ctcgtcgaca
     3061 aactgggtgg tctggatgag gccatccgga cggcggcccg gatggcccgc ctgcgcggcg
     3121 aagtcgagct cgtgacaatg ccgaggcaca caaatgtgct ggaaacggtg atccagagct
     3181 cgttacagga tgctatgggt gtgtcgaccc ggcagtcact ggccggaggg gtttacttct
     3241 tcgacccggt tgccgcgtcg ttgcgatgac acgggtggtt tttggatccg gggttttgaa
     3301 tattgcgggc cgtttctggg gttacacggt tgtacggatg tgtagaatga aggatgttgt
     3361 gaagggtacc accgggtttg cggcgccagc agcgaaatcg ctgctggctt gcgcgattgt
     3421 tttgtgcctg atgtgctccc acgcgcatgc tcaggtgggt gaattcggcc gcaacaagcc
     3481 cacatccgcc acgggaaact ggcactatgt ccagacgccg catttcgaca tctactttta
     3541 cgagggcatg gacgcgatcg cgagaatcgc cacgacgata gccgaagagg cgtattccca
     3601 gatcagtgcg agcttcgact accgcctgcg gaaccgcgtg ccgatcgtgt tgtaccggtc
     3661 ccaggggcag ttttcgcaga cgcacatcac gccctcactg atcggtgagg gtgttggcgg
     3721 gttcacggaa tttctcaaga accgcgttgc cgtgccattc accggatcct acccgcagtt
     3781 tcgtcatgtc ctccaccacg agctcgtaca cgcgatagtg cacgacatgc tttacgggcg
     3841 agacattctg accggcgtac aacagagtga tatccctctc tggttcatcg agggtctcgc
     3901 agagtacgag tcgtcgccgt ggacttcaga gagcgacatg tggatgcggg atgcggtgat
     3961 caacgattac ctgcagctcg acggttactt cgcctacaaa ggcggccagt cgatcatgta
     4021 ttacattgct gaggtgtacg ggccggacaa ggtagcgcag atcctgaacc ggcttcgctc
     4081 tttttctcgc ttcggtccga cggttcaagc cgttctgggg gtcgagctcg aagatttcgg
     4141 aaaacggtgg gagaaagacc tcaagaaggt gtactggccg gaaatcgtcg atcgagacga
     4201 gccagaggat atcggccaat cgctgacaga ccacaccaaa gacaactcct acttcaatcg
     4261 gaacccctca ttctcaccgt tcggcgacaa gatcgcgtat ttgacgggca gggattacta
     4321 caccagcatc tacgtgatgt cggccatcga cggaaaggtg ctctggaaag tcgttacggg
     4381 tgaaacttcc gggtcctttg aggaaatgca ctggcaacgg gcaggaattg cgtggtctcc
     4441 agatgcgcaa cggatagcgt ttgcggcaaa ggctacgacg ggagacaggc tttacatcgt
     4501 cgatgcgcgc aacggacgga ggctcgacgc gttcgatttc ggattcgatg gaatgttttc
     4561 gcccgactgg tccccggacg gaactacgat tgtatttgtt ggcgtgcgtg atgggaaatc
     4621 ggacctctac acacttcggc tttcggaccg gtcgattgac cggctgacgg acgactactt
     4681 tgacgagact gatccaaaat ggtcgcccga cggccggcga atcctgtttg cttccgatcg
     4741 agcgcccgat gtgagcgatg tttcagccca gttgctgtac cagacatcgg atgtgtacgt
     4801 gatggacgtc gagacgcgcg caatgcgccg cttgacggcg gaccccgcgg acgatctttc
     4861 gccggcatgg gggcccgaag ggaaacacat cgcgttcgta tccgaccgca acggtatttt
     4921 caacctgtat gttgccgcgc tcgacagcgg cgaaggaagt atcgatagtg cgacccgtcc
     4981 tttgacaaac atcctcacgg gagcgttcgc tccgtcctgg aatcgcagag gcgacaagat
     5041 cgccttctcc ggtttcaata agggggggtg ggacgttttt gtcatcagga atccactgga
     5101 cagccttcgt gaggaacccc ttcggccggc atatttcctg cgcgaaggga agatttcgta
     5161 cagcagccgc caccgccacc tggcgacgag cacgcagtcg gccacgtcac ctgattccac
     5221 cgccggattc tcgatagggc ctcgcaccag cccattcagt tcccccgccc ttgatatcct
     5281 tgctttcacc cgggcagatt ccgcgcgtgg cggcttcgtg ccaaagcctt atcgaagcca
     5341 actccaggta gacaacgtaa gcggacaggc gaattactca actctcggtg gagtcggcgg
     5401 gcagctctac gtcacggcga gcgatctcat ggggtttcac cagctccagt tcaacacgag
     5461 tctttaccgg gacctcaggg acagcgatta cggtgtgttt tatcagtatc tgaaaaggcg
     5521 aacagactac gccgcgtatt tccagcaaca acggacatgg tatcctttcc agagcgtgga
     5581 ttattacggc gggatcgtgg aatccgggtt ggtagaaagc cgggagattt caaccggcgt
     5641 ttatgcgtcc cgcccgacga gccagttccg ccggttcgaa gcgggcatag actatgtgaa
     5701 cgcgaaacag agtgtgcttt acgactacct cagctacggc accggcattg cggcgtctca
     5761 ggaagcaacc attgaggctt tgcttacaag ggttgcgttc gtgcacgaca acgcgctatg
     5821 gaacatgttt ggtccggtgg tagggcagcg ctggcgtatg acggtttttg ctgcgccgcc
     5881 gcgcgccaac accgctccat tcggcgtagt gactgcggat gtgaggcgct actggcgcgt
     5941 aagcaagaat acttcattcg cgctgcgggg ctcaggggga cggagttttg cgacgcatgc
     6001 acaaaatgcg cctctgttct atgttggcgg tttgccgtac tggatcaact atgattacga
     6061 gaacgtgcaa tccagccaac tggtgaacga cgcgttttcg cagtttgtat tcccgctgcg
     6121 gggcactaat tactacgagt tgagaggccg aagcttcgcg attctgaatg cggaataccg
     6181 atttcccctg atatatttcc ttgcgacggg cccgctcaac tttgtgctca gcaacgtcgg
     6241 cggcgtcgtg tttctggatg tcggccggac atgggaggac ccgacgcaac ccagcgaccc
     6301 gaacatagcc cgtcatgcgc agggtcccct cggcggatac ggctacggga tgcggatcaa
     6361 tctcggtgtg ctggttctga gaatggacgt agcgtggcga acagacctga gggaaacgat
     6421 cggcagcgcg aggtattact ggtctttcgg ctacgatttc tgacacaccg gtccgccgag
     6481 agt
//
LOCUS       DAOSLE010000144         6444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10882_length_6444_cov_6.863672, whole genome shotgun sequence.
ACCESSION   DAOSLE010000144 DAOSLE010000000
VERSION     DAOSLE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10882_length_6444_cov_6.863672"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /gene="mfd"
                     /locus_tag="PK576_08920"
     CDS             <1..1867
                     /gene="mfd"
                     /locus_tag="PK576_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOE61752.1"
                     /translation="IEYAEGAKLYVPVSHAHLLSRYVGVAGHKTTLHRLGGRRWTREK
                     ADAERAVADLAASLLDTQARRQVAEGLVFNPEPQWMREFEAAFPYQETPDQERVIAEV
                     KRDMAAPRPMDRLICGDAGYGKTEIAMRAAFIAVMNGRQVAVLVPTTVLAEQHYETFS
                     DRMAAYPVKIEVVSRFHSYAKRRRILTETRHGRVDILIGTHALLSEHVSFQNLGLLII
                     DEEQRFGVSHKERLKQIRQIVDVLTLSATPIPRTLYLSMTGARDMSLLQTPPHERMAV
                     ETKVARDSDTVIRNAIRLELNRRGQVYLLYNRVLTIELMRQRLEKLVPDARIAVAHGQ
                     MPSAELAHIMRAFEAGGIDVLLCTTLVESGLDIPRANTILIHRADRFGIADLYQLRGR
                     VGRSSQKGFAWLLLPEHGHVDEDARQRIAAIQKYSGFGAGFNLALRDLEIRGAGNLLG
                     TAQSGHIAAIGFNLYCQLLRRTIARLKGEALPLLVDVELALDFLDLSPGTAHDETSAC
                     LPYAYVEDEAHRMILYRRLAEAVSVKEVRALRDELTDRYGRPPAAVTRLLRLTELRIL
                     AAQKRIGRIETHNGKIHLFRSHERSPLLIRNRLPRLKGTRTDTRLDALFQTVRSL"
     gene            complement(1895..4864)
                     /locus_tag="PK576_08925"
     CDS             complement(1895..4864)
                     /locus_tag="PK576_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61753.1"
                     /translation="MNVNQRGMLCACAIAAGMVCGMPPRPEGVVPPGSALEVPVWTVA
                     EGAPQIYEHSREAGPDETLFLVGTNLTREVTLWGTDPNHLQGKSQRPAVQLASETSLA
                     LTVPERAFDGPLVLAVKNTKGFSEPIVLNAPQPWWTSPSVAQPGTLLRVFGRNLARRP
                     DHRQAFVWLAPEGARGFWLEVVEAGKYTLTVRLPDDLAPGRHALWVHAGRGGDWGWGE
                     PVTVEITAAVRLPTRSRRLRARRDALLPIQTELDALAAKGGGTLYLPAGTHEFRGTLV
                     IPEGVVLRGAGREATTLQLVPGPAGDFTRFVTEAWGAGPSSVHTPGDEITYEVTIPEA
                     GRWQIWLRYGTDMSPWKLPGVSGHAALRFGEGPPVTLENLPNTGGFGVFKWSRSAEVE
                     LKAGRQTMVWRNIHGRGITLDAFVLRRESASAPSDKPWPVNGPGCVVIQAEACSRFQC
                     REGSLPNLNRAAVWLAGDGAAIESLTVLGQPQVNQGIAIRATGQTEWVNGCRVRDVRV
                     ADCEGKQTENCGIYLRQVRHSLICGNELWGRTPLFLSGVRDTVFVSNRLVSVTRYGGN
                     SEGAILGRTEPIERCVIERNTVASPAGAEAGGPTARRLIWLSTGHGSVTHNWLAWNGA
                     EAEGAAGQARFGGVAGTDQNVGEMILFEANHRTMYFGPLAGADAESVTLPLTLPPTPD
                     ALLGSVKREALAYDAAGRETPFWPPDVDDGTPEPPISEYYVTVFAGMGQGQTRRVVSR
                     EGGRLQLNQPWRVTPQAGSVVAVGTMFYRNLIVGNRTMDGMSGIQLWISCVENVVAGN
                     TIARQRKQGLFFYANGSTLASSMPRTWNRGISPLFWNLAEGNRTEECSDGVLVTSGDA
                     PNLPIAFPRALGNVLRHNSFIRSRHNGVILTSRKRGGGDDSASVVGTVVEFNVVRDAP
                     VAYRAAGGSDVVVLRRNHAYAWHPALHAPEPPVAFRIDRSATHCALEANSVEGRSGVK
                     DNNVREVVRDGEAEKGK"
     gene            5369..6214
                     /locus_tag="PK576_08930"
     CDS             5369..6214
                     /locus_tag="PK576_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE61754.1"
                     /translation="MWLLIAASFLFSFSFGLIGNRLAGLPPAWLAAARLTVAAAVFLP
                     FARRVPFRTGAALFAVGMVQFGLMSLTYMASFRFLASHEVALFTVMTPLYVTVLHDLH
                     RRAFHPVNLLAASLAVAGAALILWRGRLSGAPLTGFLLVQASNLCFAAGQIAYRALTS
                     AARAPLSDRQVFFWLYLGGLTALLPFGLRDALLLAPRPTLTQLAVLLYLGVAVSGLSY
                     FLWNAGARRVSSGVLAVMNNIKIPLGMLVSLTCFGEPVQHASLLAGGTLILAALLPPL
                     LRRKA"
     gene            complement(6278..6439)
                     /locus_tag="PK576_08935"
     CDS             complement(6278..6439)
                     /locus_tag="PK576_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61755.1"
                     /translation="MTGALTEIKNQVGRTVDQYYKFSMILKDLRSGELVWADEQEIRK
                     ESVKPRVGF"
BASE COUNT         1087 a         2380 c         1856 g         1121 t
ORIGIN      
        1 gatcgaatac gcggagggcg ccaaactgta cgtgccggtc tcgcatgcgc acctgctcag
       61 ccgctacgtg ggcgtggcag gccacaaaac cacgctccac cggttgggcg gacgccgctg
      121 gacgcgcgaa aaggccgatg ccgaacgcgc ggtcgcggat ctggccgcgt ccctgctgga
      181 cacccaagca cggcggcagg tcgccgaagg cctcgtcttt aatcctgaac cgcagtggat
      241 gcgcgaattc gaagccgcct tcccgtatca ggagaccccc gaccaggagc gcgtgatcgc
      301 cgaggtgaag cgcgatatgg ccgcaccgcg cccaatggac cgcctcatct gcggcgatgc
      361 cggttacggc aaaaccgaaa tcgcaatgcg ggccgccttc atcgcggtga tgaacggccg
      421 tcaggtggcc gtgctggtcc ccaccaccgt cctcgccgaa cagcactacg aaaccttttc
      481 cgaccgcatg gccgcctatc ccgtgaagat cgaggtggta agccgcttcc attcgtacgc
      541 caaacggcgg cgcatcctta ccgagacccg ccacggccgc gtagacattc tgatcggcac
      601 gcacgccctg ctgtcggaac acgtttcttt ccagaacctc ggcctgctca tcatcgacga
      661 ggaacaacgc ttcggcgtgt cccataagga acggctgaag caaatccggc agatcgtgga
      721 tgtgctcacg ctctcggcca cgccgatccc gcgcacgctc tacctgagca tgaccggcgc
      781 gcgcgatatg agtctgctgc agaccccgcc gcatgaacgc atggccgtgg aaaccaaggt
      841 ggcgcgcgat tccgacaccg tgatccgcaa tgcgatccgt ctcgagctga accgccgggg
      901 ccaggtctat ctcctttaca accgcgtgct caccatcgag ctgatgcggc aacggctgga
      961 gaagctggtt cccgatgcgc ggattgccgt ggcgcacggc cagatgccgt ccgctgaact
     1021 ggcccacatc atgcgcgcct tcgaggcggg ggggatcgac gtgctgctgt gcaccacgct
     1081 ggtggagagc ggcctcgaca tcccgcgcgc caacacgatc ctgatacacc gcgccgaccg
     1141 tttcggtatc gccgacctct accagctccg cggccgggtc ggccgctctt cgcaaaaggg
     1201 gttcgcctgg ctgctgctgc ccgaacacgg tcacgtggat gaggacgcgc gccagcgcat
     1261 cgccgcgatt cagaagtaca gcgggttcgg cgcgggcttc aacctcgcgc tgcgcgacct
     1321 cgagatccgc ggcgcgggaa atctgctggg caccgcacag agcggccata tcgcggccat
     1381 cggctttaac ctctactgcc agttactgcg acgcacgatc gcgcgcctga agggcgaagc
     1441 cctgccccta ttggtggatg tggaactcgc gctcgacttc ctcgacctct cgcccggcac
     1501 agcgcacgac gagacctcgg cctgtctgcc gtacgcgtat gtggaggatg aggcgcaccg
     1561 catgatcctt taccgacgcc tcgcagaggc ggtcagcgtg aaagaggtcc gcgcgttacg
     1621 cgacgaactg accgaccgct acggcaggcc gcccgccgca gtcacccgcc tgctgcggct
     1681 gacggaactg cgcattctgg cggcgcaaaa acggatcggc cgcatcgaga cgcataatgg
     1741 caaaatccac cttttccgct cgcacgagcg ctcgccgttg ctgatccgga accgactccc
     1801 ccgcctcaag ggaacccgga ccgacacgcg tctcgacgcc ctgttccaaa ccgtgcgctc
     1861 gctctgaccc gcgggacgac actctccccg cctgtcactt tcccttttcc gcctccccgt
     1921 cccgtaccac ctcgcgcacg ttgttatcct tcacacccga acggccctcc accgagttcg
     1981 cctccagcgc acaatgcgta gcggaacggt cgatccggaa cgccaccggc ggttccggcg
     2041 catggagggc gggatgccag gcgtaagcgt gattgcggcg cagcaccacg acgtcactgc
     2101 ccccggccgc gcggtaggcc accggcgcgt cgcgcaccac gttgaactcc accaccgtgc
     2161 cgaccaccga agccgagtcg tccccgcccc cccgtttgcg actggtcaaa atgaccccgt
     2221 tatgccgcga gcggatgaac gagttgtggc gcagcacatt acccagcgcc cgcggaaagg
     2281 cgatcgggag attcggcgcg tcaccgctgg tgaccaacac gccatcgctg cactcttccg
     2341 tgcggttccc ctcggccaga ttccagaaaa gcggggagat gccgcggttc caggtgcgcg
     2401 gcatggacga ggccaacgtg ctcccgttgg cgtagaaaaa cagtccctgc ttgcgttgac
     2461 gcgcgatcgt gttccccgcg accacattct ccacgcacga gatccagagt tggatgccgg
     2521 acatgccgtc catcgtacgg ttcccgacaa tgaggttacg gtagaacatg gtgccgaccg
     2581 ccacgaccga accggcctgc ggcgtgaccc gccacggttg gttaagctgt agccgccccc
     2641 cttcacgtga caccacgcgc cgcgtctgcc cctgacccat gcccgcgaac accgtgacgt
     2701 aatactcgct gatcggcggt tccggcgtgc cgtcgtccac gtccggcggc cagaacggcg
     2761 tttcgcgccc cgccgcatcg tacgccaggg cctcacgctt gacactgccc agcagggcgt
     2821 ccggtgtggg cggcagggtt agcggcagcg tcacactttc cgcatccgcg ccagccagcg
     2881 gaccaaagta catcgtgcgg tggtttgcct cgaataaaat catctcgccc acattctgat
     2941 ccgtgcccgc cacgccaccg aaacgcgcct gacctgcggc accctccgcc tcggcgccgt
     3001 tccaggccag ccagttgtgc gtgaccgagc cgtgtcctgt cgagagccag atcagccgcc
     3061 gcgcggtagg gccgcccgcc tccgccccgg ccggtgaggc gaccgtatta cgctcgatca
     3121 cgcaccgctc gatcggctcc gtgcgcccga ggattgcacc ctcggaattg ccgccgtaac
     3181 gggtaaccga aaccagccgg ttcgagacaa acaccgtatc gcgcaccccc gagagaaaga
     3241 ggggggtacg tccccaaagc tcattgccgc aaatcaggct gtgccgcacc tgccgaaggt
     3301 agatgccgca gttctccgtc tgcttgccct cgcaatccgc cacgcgcaca tcccgcacgc
     3361 ggcagccgtt cacccattcg gtctggcccg ttgcccgaat cgcgatcccc tgattgactt
     3421 gaggctgccc aagcaccgtc aggctctcga tggccgcccc gtctccggcc agccagaccg
     3481 cagcccggtt gaggttcggc agcgaacctt cgcggcactg gaaccgcgag cacgcctcgg
     3541 cctggatcac cacgcacccc ggcccgttca ccggccacgg cttgtccgaa ggcgcggagg
     3601 ccgactcccg cctcagcaca aacgcatcca gcgtaattcc cctgccatga atgttccgcc
     3661 acaccatcgt ctgccgtccc gccttgagtt ccacctccgc agagcgggac cacttgaaca
     3721 caccgaaccc tccggtgttg ggcaggttct caagcgtcac gggcgggcct tccccgaacc
     3781 ggagggccgc atgtccgctg acgcccggca gtttccaagg cgacatatcg gtaccgtagc
     3841 gcagccagat ctgccaccgg cctgcctccg ggatcgtcac ctcgtacgtg atctcatcgc
     3901 ccggggtgtg cacactgctg ggacccgcgc cccaggcctc ggttacgaaa cgcgtgaaat
     3961 ctccggctgg cccgggcacg agctggagcg tggtggcctc gcgtcccgca ccgcgcaaga
     4021 ccaccccctc cgggatcacc agcgtccccc ggaactcatg tgttccggcg ggcagataca
     4081 gcgtgccgcc gcccttggcg gccagcgcgt ccagttccgt ctggatcggc aacaacgcgt
     4141 cccgccgcgc gcgcaaccgc ctgctccggg tgggcaaccg caccgccgcc gttatttcaa
     4201 cggtcaccgg ttcgccccaa ccccaatccc cgccgcgccc ggcatggacc caaagcgcat
     4261 ggcgaccggg cgcgagatcg tccggcaaac gaaccgtcag cgtgtacttc cccgcttcaa
     4321 ccacttccag ccaaaagccc cgcgcgccct ccggagccag ccagacaaac gcttgacggt
     4381 gatcgggccg acgcgccaga ttgcgcccga acacgcgcag caaggtgccc ggttgcgcga
     4441 ccgaaggcga ggtccaccac ggttgcggcg cattgagcac gatcggctcc gaaaagcctt
     4501 tcgtgttttt gaccgccaaa accagcggac cgtcaaacgc ccgctccggc acggtcaacg
     4561 ccagcgaggt ttccgaggcc agttgtacag cggggcgctg cgacttgccc tgcagatggt
     4621 tcggatcggt tccccatagc gtcacttcgc gcgtcaggtt ggtgccgacc aggaaaagcg
     4681 tctcatccgg tcccgcctcg cggctgtgct cataaatctg cggcgctccc tcggccacag
     4741 tccagaccgg cacctccagc gcgctccccg gcggcaccac accctcaggc cgcggcggca
     4801 tcccgcacac catcccggcc gcaatcgcgc atgcgcacaa catcccccgc tggtttacat
     4861 tcatcacatc cgcctccctt ttccattccg tcctgttgcc ctcagtttcc ccttttctct
     4921 ctgctttgtc gagggatgac aggaaaaaag gcacgtctga cttgcgaccc gcgacattcg
     4981 acctgcgacg atcgacaggg acgcccgctg gtagggacgg ttccccgaac cgtccgcgga
     5041 cgccttaaaa agagcagttg acccttcaac cctctttggt ttcaaggttt cagggtttcg
     5101 gggtttcagg gtttcagggt ttcagggttt cgggtttcag ggtttcatcc aaatcgggat
     5161 cggtatcgct atcggtatcg ctatcggtat cgggatcgac tgcttaacca cagaacacaa
     5221 ccccctcaca gcctcacccc ctcaccccct cacagcctca ccccctcacc ccctcacagc
     5281 ctcaccccct aacccctcac cccttctggc cgtccgcgcc gatcaaatgg actcgccgcg
     5341 accgccgcgc ttttgataca aacggggcat gtggctcctg atcgcggcat cattcttgtt
     5401 cagtttctcg ttcggactga tcggtaaccg tctggccggg ctcccgcccg cctggctggc
     5461 cgccgcgcgc ctaaccgtgg cggctgcggt cttcctgccc ttcgcccgcc gcgtgccgtt
     5521 ccgaaccggt gccgccctct tcgcggtcgg catggtccag ttcggcctga tgtccctcac
     5581 ctacatggcc agcttccgct ttctcgcctc gcacgaggtg gcgctcttca cagtcatgac
     5641 ccccctctac gtgacggtgc ttcacgatct gcaccggcgc gctttccatc cggtcaacct
     5701 gttggccgca tcgctcgcgg tggcgggagc ggctctgatc ctgtggcgcg gccgactgtc
     5761 aggcgcgccg ctgaccggtt ttctgctggt ccaggcttcg aacctctgtt tcgccgcggg
     5821 ccagatcgcc taccgtgcgc tgaccagcgc ggcacgcgcg ccgctttccg accgtcaggt
     5881 gtttttctgg ctctacctcg gcggactgac cgccctgctg ccgttcggcc tgcgcgacgc
     5941 gctgctcctc gccccgcgcc ccaccctgac gcaactcgcg gtgctgctct atctgggcgt
     6001 ggcggtctcc ggcctcagct attttctttg gaatgctgga gcgcggcgcg tttcatccgg
     6061 cgtgctggcc gtcatgaaca acatcaaaat cccgctcggc atgctggtct cgctgacgtg
     6121 tttcggcgaa cccgtgcaac acgccagcct gctcgcgggc ggcacgctga ttctcgccgc
     6181 gctgctccct ccgctcctcc gccgcaaggc ctgaacgcgc ggcacgcggc cgcacccctg
     6241 cggccgcgcc acaacccgtc atgccggacg gatccgctca gaagccgaca cgcggcttga
     6301 cgctctcctt gcggatctcc tgctcgtccg cccaaaccag ctcgccggaa cgcaggtcct
     6361 tgagaatcat cgagaacttg tagtactggt ccacggtgcg gccgacctga ttcttgatct
     6421 cggtcagcgc gccggtcaga taca
//
LOCUS       DAOSLE010000145         6438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10895_length_6438_cov_7.154629, whole genome shotgun sequence.
ACCESSION   DAOSLE010000145 DAOSLE010000000
VERSION     DAOSLE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10895_length_6438_cov_7.154629"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PK576_08940"
     CDS             complement(<1..434)
                     /locus_tag="PK576_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE61756.1"
                     /translation="MADVTQQRVRCGLCPKRCAIPPGHSGECRVRINADGVLRAVTYG
                     RPSAVHVDPMEKKPLFHFFPGAAILSLGTVGCNFHCKNCQNAELSQGDPETMPAYDLQ
                     PAALPETARRQGCAHVAYTYNEPLVSYEYVRDCCRAARDAGLR"
     gene            complement(634..1524)
                     /locus_tag="PK576_08945"
     CDS             complement(634..1524)
                     /locus_tag="PK576_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOE61757.1"
                     /translation="MFFGYLTVGIVVTLLAVAGSAAASEEPIRMRVIADRAPVCTATN
                     PTRETVLWLPSGAEVNLIGELVDGQEWVRVTAPPALALWIYRELVRDGRVIADKSQIR
                     TGPGQAFPVVAQLIRGEPVTVRGTYGDWLKIVPPAQQSFWMMRDNVEPLAFPAPEGES
                     DVADVPGGPASEGEGPVEAAPEVSLPAPAPIVERFRPPVPSELSAFVLDEKREQGRPA
                     ERRGILDWGGVGSVSTPFCLVGRRGAGEESAPVCHLILPVATYGPHIGAALSITGTEW
                     YVNGSALPVLIPTRVRMDIP"
     gene            complement(1645..1800)
                     /locus_tag="PK576_08950"
     CDS             complement(1645..1800)
                     /locus_tag="PK576_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HOE61758.1"
                     /translation="MSQHTSLKGSGKITVKRNVLKRFERVNLLKKRGQWKDGDRGLGL
                     PKTKPEV"
     gene            complement(1898..3292)
                     /locus_tag="PK576_08955"
     CDS             complement(1898..3292)
                     /locus_tag="PK576_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009966024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61759.1"
                     /translation="MTTSRRSFLKRATFTGVPFLLPGRIWANAVKPNDKPGMAFIGMG
                     IQARALLNNFLRQDVNVLAVCDVDTTRREDARVKVDTFYKEHPEKGVPGNCKAYNDFR
                     EVLARKDISLVCVATPDHWHAYITIEAMRSGKDVYCEKPLTYSINEAVAVMEATRRYN
                     RILQTGAMQRSGFEFYTAAELVRNGCIGKITHVECSFGGPSRPHVDPQEDIPMEPGLD
                     WDMWCGGAPLVKYNDKLSPRGIHNFYPMVWRMDDLFGSGMCGDWGAHHLDIAQWGLGM
                     DAGGPVEVIRAERTEKDKANVALGGRAQSGVVLRFADGVTVKHTRLPIFGTVFHGTEG
                     TVSVNRGKFEMTHGKELISRFTKKEDGGSLEGALMKARKAFLTDSTYKVKLYRTKGGH
                     PADFVTCAKSRQRACSNEDVGGRAAILCHLCNLSYMRDASFRWDPVRNTFASGSGNPA
                     WLGREGGYRGGWSV"
     gene            complement(3407..5086)
                     /gene="ettA"
                     /locus_tag="PK576_08960"
     CDS             complement(3407..5086)
                     /gene="ettA"
                     /locus_tag="PK576_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOE61760.1"
                     /translation="MAGEYAFSLINVTKQHENKIILEDVNLSFFHGAHIGVIGANGSG
                     KSSLLKILAGLDKEFMGECQVAKNTRVGYLAQEPELDDSKNVMQCVEEGVAGSKALLA
                     RYDEICEKLGEDMSDEESEKLNDELGRLQDIIDQNDLWSLDHQVEMAMEALRLPPPEA
                     PVAPLSGGERRRVALCRLLISNPDVLLLDEPTNHLDAESVAWLETYLKGFKGTLIVVT
                     HDRYFLNNVTEWILELDNGRTYPYKGNYEMWLVQKQEAMLRESKQAESRRKLIENELA
                     WVRTNPSGRRAKNQARVRRYEELIAGKVSTREDDLRIQIPAGPRLGDLVVRAEAVAMG
                     FDDRLLFEDMNFDLPRGGIVGVIGGNGAGKTTLFKLITGQLQPLSGTLTVGPTVQISY
                     VDQSRAELDPARTVYEEITGGSEYVSLAGEAMMNGRAYCSRFNFRGGEQQKLVGVLSG
                     GERNRVHLAKLLRSGGNLLLLDEPSNDLDVATLRSLEEGIADFGGCVMVISHDRWFLD
                     RIATHILAFEGDSVVTWFEGNYADYHEQRRKLLGEAADRPTRVRFRPLKHG"
     gene            complement(5234..6373)
                     /locus_tag="PK576_08965"
     CDS             complement(5234..6373)
                     /locus_tag="PK576_08965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE61761.1"
                     /translation="MGILTAGGDCPGLNAAIRAVAKSAMHYDIQVVGFLDGFRGLVEN
                     RYIQLEDRTVSGIITLGGTILGSSRDKPHKMNMGGTIMDMTDVAVANADRLNLDCLVC
                     LGGNGTQKNAYRLSQKGLNIITLPKTIDNDVVLTDTCFGYDTAKSIATEAIDRLHTTA
                     SSHHRLIAVEIMGHSAGWLALGAGIAGGADVILIPEIKYDIHAIADFINTRCHRGKRF
                     SIIAVAEGAIAIDDKPEDDEEEEDSDSKPQKKNGKKKNGDTNGSTPVKLPQKPLMIRE
                     SKVSRIVRELQQLTGIDARMTQLGHIQRGGTPTPADRLLCSRLGAMAGELINAREYNV
                     MVAVRGDDCVAVPLKDVAGHVRTVPRDHPWIKTARLLGTCMGDAS"
BASE COUNT         1272 a         2102 c         1793 g         1271 t
ORIGIN      
        1 cgcaatccgg catcccgcgc agcccggcag cagtcgcgga catactcgta actaaccagc
       61 ggttcgttgt aggtgtaagc cacatgcgcg cacccctgtc tgcgggcggt ttcgggcaac
      121 gccgcgggct ggaggtcata tgcgggcatc gtctcgggat caccttgcga caactccgcg
      181 ttctgacagt tcttgcagtg gaaattgcat ccgaccgtac ccagcgaaag aatcgccgca
      241 cccggaaaaa aatggaaaag cggctttttc tccatgggat caacatgcac ggcgctcgga
      301 cgcccgtagg tcaccgcccg caagacgccg tccgcattga tccggacacg acactccccg
      361 ctatgcccgg ggggaatcgc gcaccgcttc ggacacaggc cacatcgcac acgctgttgg
      421 gtcacatcag ccatcacaca ccttgcttac gggataaagc ataccccatc tccgcgaaaa
      481 agtgaagcgg tcccatgaac gtcgacttgc gacccgcgac attcgccctg cgacgtctgg
      541 gttgtttggc aaaggaatga tctaactgat cagtccgatc cgacgaccca tcagcgggca
      601 aggacgcccg cgcacccgcc tcctgtcttc tgtctacggg atgtccatac ggacccgggt
      661 cggaatcagc accggcagtg ccgacccatt aacgtaccac tccgttcccg taatcgaaag
      721 ggcggccccg atatgcggac catatgtggc gaccggcaga atcaagtggc aaaccggtgc
      781 cgactcttcc ccggccccac ggcgtcccac cagacagaac ggcgtcgaaa cagatcccac
      841 gccaccccaa tcgaggatcc cgcgccgttc ggccggacgt ccctgctccc gtttctcatc
      901 caacacgaat gcgctgagtt cagacggcac ggggggacga aaacgttcca caatcggcgc
      961 gggggcggga agagaaactt ccggcgccgc ttcaacaggc ccctccccct ccgaagcagg
     1021 gccccccggc acatccgcga catccgactc cccctccggt gcggggaagg ccaacggctc
     1081 cacgttatcg cgcatcatcc aaaaagactg ttgcgcggga ggcacgattt tgagccagtc
     1141 accgtacgtg ccgcggaccg ttacgggctc cccccggatc aattgcgcca caaccggaaa
     1201 agcctgcccc ggtccggtcc ggatttggct cttgtccgca atcacacgcc cgtcccgcac
     1261 cagctcccga tagatccata acgcgagggc ggggggcgcc gtgacccgaa cccactcctg
     1321 cccgtcgacc agttctccga tcagattcac ctcggcaccg gacgggagcc ataacaccgt
     1381 ttcgcgtgtt ggattcgtcg ccgtgcagac cggtgcgcgg tcagcgatca cccgcatccg
     1441 gattggctct tccgacgcgg cagccgaccc cgccaccgcc aacagggtca cgacgatccc
     1501 aaccgtgagg tatccgaaaa acatgacaac ccctccttcc gccgtaccat gccgaaaccg
     1561 aaaaaaccgc atgcgcattg cccgcaaaag cgggccgcgc atgcggcgga aaccgacacg
     1621 aaaaaacgtc gatcggtgat gcggctacac ttccggcttg gtcttgggca accccaagcc
     1681 ccgatccccg tctttccact ggccacgctt cttgagcaga ttcacgcgct cgaaacgctt
     1741 caacacattc cgcttcacgg taatcttgcc tgaacccttt aaactggtat gctgactcat
     1801 gactgcttcc tctcctccga acaaacgcct tccgtaaaag gcagaaaacg aagtagaata
     1861 ctaaaaaaag gatgtagatc aaccgcaaaa agccaggtca caccgaccag ccgccgcggt
     1921 atccgccctc gcgaccgagc caagccggat tccccgagcc ggacgcaaag gtgttccgga
     1981 ccggatccca gcggaaactc gcgtcgcgca tgtacgacag gttgcacaga tggcacagga
     2041 tcgcggcgcg cccgccgaca tcctcattgg aacacgcgcg ctgacgcgat ttcgcgcagg
     2101 tcacgaagtc ggcagggtgt ccgcccttag tccgatacaa cttcacttta taggtgctgt
     2161 ccgtaagaaa ggccttacgg gctttcatca gcgcaccttc gagtgacccg ccatcctcct
     2221 ttttggtgaa gcggctgatc agttccttgc cgtgcgtcat ttcaaacttg ccgcggttca
     2281 ccgacaccgt gccttcggtg ccgtggaaga ccgtgccgaa gatgggcagc cgagtgtgtt
     2341 tgaccgtcac gccgtcggcg aaacgcagca ccacaccgga ttgcgcgcgg ccgcccagcg
     2401 ccacgttggc cttatccttc tccgtgcgtt cggcccggat gacctcgacc ggtccgcccg
     2461 catccatacc caacccccat tgcgcgatat cgaggtgatg cgcgccccag tcaccgcaca
     2521 tcccggaccc gaacaggtcg tccatgcgcc agaccatggg gtaaaaattg tggatcccgc
     2581 gcggcgacag cttgtcgttg tatttgacga gcggcgcacc gccacaccac atatcccagt
     2641 ccaaccccgg ctccatcggg atatcctcct gcggatcgac atgcggacgg gacgggccgc
     2701 cgaagctgca ttcgacatgg gtgattttcc caatgcaccc gttacgcacc agttcggcag
     2761 cggtgtaaaa ctcaaacccg gaacgctgca tcgcgccggt ctgaaggatc cggttgtacc
     2821 gtctggtcgc ctccatgacc gccaccgcct cgttgatgga ataggtcagc ggcttctcgc
     2881 aatacacatc cttgcccgaa cgcatggctt cgatggtgat gtaggcgtgc cagtggtccg
     2941 gtgtggcgac gcagacaaga ctaatgtcct tgcgcgcgag cacctctcgg aaatcgttgt
     3001 acgccttaca gttgccgggc accccctttt cgggatgctc tttgtaaaac gtatccacct
     3061 tgacccgcgc gtcctcacgg cgtgtcgtat cgacatcaca caccgccagc acattcacgt
     3121 cctgacgcag gaaattattc aagagcgcgc gcgcctgaat ccccatgccg atgaaggcca
     3181 tgccgggttt gtcattgggc ttcaccgcat tggcccagat ccgccccggc agcaaaaacg
     3241 ggacacccgt gaacgtcgcc cgcttgagaa aacttctgcg tgatgtcgtc atcctctccc
     3301 ccttgtttcg atcaacgttt ttccgtacaa aagtttgaaa atgatatcat gcgtgattgc
     3361 ggaaaggcaa gcgggtttcc gcggtttcca acgcgccgtt ttcgcgtcac ccgtgcttca
     3421 acggacggaa ccggacccgg gtgggacggt cagccgcctc gccgagaagt ttacggcgct
     3481 gttcgtggta atcggcgtaa ttgccttcaa accacgtcac cacgctatcc ccctcaaagg
     3541 cgaggatgtg cgtggcgatg cggtcgagaa accagcggtc gtggctgatc accatcacgc
     3601 atccgccgaa atcagcgatg ccctcctcca gtgaacgcag ggtggcgaca tcaagatcat
     3661 tggagggttc gtcaaggagc agcaaattgc cgccgctgcg cagcagtttg gccagatgca
     3721 cacggttacg ctcgccgccg gacagcaccc ccaccagctt ctgctgttcc ccgcccctga
     3781 aattgaaacg gctgcaataa gcccgcccgt tcatcatcgc ctctcccgcc agactgacat
     3841 actccgagcc gcccgtgatt tcctcatata ccgtcctggc cgggtccagt tccgcgcgcg
     3901 actggtcaac gtacgaaatc tgcaccgtag gcccgaccgt caaagttccg gacagcggct
     3961 gcaactggcc cgtgatcagc ttgaagagcg tggttttgcc cgcgccgtta ccgccgatca
     4021 cccccacgat gccgccgcgc ggcaaatcaa agttcatgtc ttcaaaaagc agccggtcgt
     4081 caaaccccat cgccaccgcc tccgccctga ccaccagatc gcccagccgc ggtccagcgg
     4141 gaatctgtat gcgcaggtca tcctcgcgcg tgctcacctt tccggcaatc agttcctcgt
     4201 agcggcgtac acgggcctgg ttcttggcgc ggcgtcctga cgggttggtg cgcacccaag
     4261 ccagttcgtt ctcgatcagc ttgcgccgcg attcagcctg cttggactcg cgcagcatgg
     4321 cctcctgctt ctgcaccagc cacatctcgt aattgccctt gtagggatat gtccgcccgt
     4381 tatcaagctc cagtatccac tccgtcacat tgttcaggaa gtaacggtcg tgcgtcacca
     4441 cgatcagcgt gcccttgaac cccttcaggt aagtttccag ccatgccacg gactcggcat
     4501 cgagatggtt ggtcggctcg tccagcagca gcacatccgg attggagatc agcaggcggc
     4561 aaagcgccac acggcggcgc tcgccgcctg agagcggtgc caccggcgct tcgggaggcg
     4621 gcaaccgcaa agcctccatg gccatctcca cttggtggtc gagactccaa agatcgttct
     4681 gatcaatgat gtcctgcagc cggcccagct cgtcgttgag tttctccgac tcctcgtcac
     4741 tcatgtcctc gcccagcttt tcgcagatct catcataacg ggcgaggaga gccttcgacc
     4801 ccgcgacccc ctcttcaaca cactgcatga cgttcttcga gtcgtccagt tcaggctcct
     4861 gcgccaaata ccccacccgc gtattcttgg cgacctggca ctcgcccata aactccttgt
     4921 ccagacccgc caaaatcttg agcagcgaag atttgccgga accgttcgcg ccgatcaccc
     4981 cgatgtgcgc cccgtggaaa aacgagaggt tcacatcctc aaggatgatc ttattctcat
     5041 gctgcttggt cacgttaatt aacgaaaacg catattcgcc ggccatgggg actccttacg
     5101 ttcttatcct ctgcatccag ccttcacaat aggcgcgaat tttcggcaag cataccaacc
     5161 gcccccctca aagtccagcc aatgggcttg caggcaagga gaaggtcatt cgttcaatcg
     5221 tagtgggcgc gagttaagac gcatcgccca tgcaggtgcc gaggaggcgc gcggtcttga
     5281 tccagggatg atcccgcggc acggtgcgga catgcccggc gacatccttg aggggcacgg
     5341 cgacacaatc gtcaccgcgc acagcgacca tgacgttgta ctcgcgggcg ttgatgagtt
     5401 cgccggccat tgcgccgagc cgactgcaga gcaagcggtc ggccggggtg ggcgtgccgc
     5461 cgcgctggat gtggccaagt tgggtcatgc gcgcatcgat gccggtgagt tgctggagtt
     5521 cacggacgat acggctgact tttgactcac gaatcatgag aggcttctgc ggcagcttca
     5581 cgggggtgct gccgttggtg tcgccattct ttttcttgcc gttctttttc tgcggcttag
     5641 agtcgctgtc ctcctcctcc tcatcatcct cgggtttgtc gtcgatcgcg atggcacctt
     5701 cagccacggc gatgatggaa aaacgcttgc cgcggtgaca acgggtattg atgaaatcag
     5761 cgatggcgtg gatgtcgtat ttgatctccg ggatcaggat aacgtcggca ccgcccgcga
     5821 tgccggcgcc gagtgccagc cagccggcgg agtggcccat gatctcaaca gcgataaggc
     5881 ggtggtggct gctggcagtg gtgtgaaggc ggtcgatcgc ctcggtcgcg attgacttgg
     5941 cggtgtcgta accgaagcag gtgtcggtca ggacgacgtc gttgtcgatc gtcttcggca
     6001 gcgtgatgat attgaggccc ttttgcgaga gacggtaagc gtttttctgt gtgccgttgc
     6061 cgccgaggca cacgagacaa tcgaggttga gtctgtcggc gttggccacg gcgacgtcgg
     6121 tcatgtccat gatggtgccg cccatgttca ttttgtgcgg cttgtcacgg cttgagccga
     6181 ggatcgtgcc gccgagcgtg atgatgccgc tcacagtgcg gtcttcgagt tggatgtagc
     6241 ggttttcaac gaggccgcgg aagccgtcga gaaaaccgac aacctgaatg tcgtagtgca
     6301 tggccgattt ggccaccgcg cggatcgcgg cgttgaggcc cgggcagtcg ccgcctgcgg
     6361 tcaggatgcc gatgctgcgt gcgggtttac cgttttgtct cttgttgtcg ttgctcaaag
     6421 ttttacgtcc gtttgcgg
//
LOCUS       DAOSLE010000146         6408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10956_length_6408_cov_7.715253, whole genome shotgun sequence.
ACCESSION   DAOSLE010000146 DAOSLE010000000
VERSION     DAOSLE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10956_length_6408_cov_7.715253"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PK576_08970"
     CDS             complement(<1..511)
                     /locus_tag="PK576_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOE61762.1"
                     /translation="MAMTEALSISIEDYLEAIFYLVRENTVARSKDIAERLKVNRSSV
                     TGMLQALRDRGLVNYERYGFVTLTDEGKKVASRVVRRHEALRDFMVHVLSIDEAEADQ
                     AACHMEHGVSSHIVERFTEFADFVKTCPRAGAKWVRGFGYRCEEAAVEPQNCERCIEL
                     CLEDVRKKPK"
     gene            complement(611..817)
                     /locus_tag="PK576_08975"
     CDS             complement(611..817)
                     /locus_tag="PK576_08975"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61763.1"
                     /translation="MHPRLGRVTAAGVEVDIRGTADPDSDVRINGVAATREGDAFSST
                     VLLRTGGNMVDVQAHKPGAEPTAP"
     gene            complement(828..1691)
                     /locus_tag="PK576_08980"
     CDS             complement(828..1691)
                     /locus_tag="PK576_08980"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61764.1"
                     /translation="MTRGHGAWWGGAAVAGWLALATQAAPVTEKVDVIDLIRQKQTEV
                     HLGPKSEFSDKPSEIFQVREDGMLHISGRGYGYLATQQAYQDYHLVVEFRWTGATCGK
                     RVDKARDNGILVHAHGPHGSCGGVWIASVEAQIIEGGMGDIIVVSGKQPDGTPVTTSI
                     EAEIELDRDGEKRWKKGAPRQTLTGGRVNWEKRDEDWKDLLGFRGLYDLDAPVGDWNR
                     LEVIARGDTLRYFFNGRLVNEAFAVKPSAGRVCIQTEGAEMIVRRFELWPLDTFKEAW
                     KPVVQVTDAID"
     gene            1874..3130
                     /locus_tag="PK576_08985"
     CDS             1874..3130
                     /locus_tag="PK576_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOE61765.1"
                     /translation="MKALAKYSMGIGDRFGRQGEAQLEAFKRFREQCGVEVVPVWNKS
                     DREHKTIGTRPESVRQEADAAAAKVGWTAPYHVDADHIRLATVDGFIASSDFFTLDVA
                     DYMGETTDEAEIYGFVARHEELIGSHAIEGLESPVTLTKPQMAEIVRGTLFAIRQAGR
                     LYRHIAEQKGSEPFITEISMDETTQPQTPEMLLVILAVVAEEQIPAQTIAPKFTGRFN
                     KGVDYAGDAALFGREFDADVCVVRYAVQHFGLPRNLKLSIHSGSDKFSLYPYVARTLQ
                     ARDAGVHVKTAGTTWLEEVTGLAVSGGEGLAIAKEIYLKALPRFDELCGPYATVLDIS
                     PERLPSPDEVASWNGGRFAASLRNVPGDAHFNPDFRQFIHVAYKVAAELGERYLTALD
                     ENRETVARHVTENLLTRHLKPIFRGA"
     gene            3367..3966
                     /locus_tag="PK576_08990"
     CDS             3367..3966
                     /locus_tag="PK576_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE61766.1"
                     /translation="MTQETEDPRLAAIFTSFAHVGAFLFGGGYAMLPLIEREVVNRRG
                     WCRFEEMADYYAMAQLVPGVIAVNTAMLIGHRLRGFRGAAAAMLGVVCAPFFVILAYA
                     VAVDRLAGMPFLERALAGLRPAVAGMMLGIAYTMFNRTRRTRLGLTVCLVTTALVLGA
                     GVSAVAVILLGVTAGLVGYGVRKGLRGSVSDAENSGGQP"
     gene            3963..4547
                     /locus_tag="PK576_08995"
     CDS             3963..4547
                     /locus_tag="PK576_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE61767.1"
                     /translation="MNLLLKLASVFFKIGLLTVGGGLAMIPIVQQEMVGRGWLTNRQF
                     LDILGIAQMTPGPMSVNTATFVGYRVVGTEYPGAVWLAVAGSVVATLAVCAPSLLCIN
                     LLGAFWMRNREHPCLVSVFTFLRPLVTGLVIHAALLLGVNCLWADPVREWLSQSPDVR
                     AGVLLVVAFLLTVLTRVSPVLVLLGGAIVGLLIF"
     gene            complement(4509..5468)
                     /locus_tag="PK576_09000"
     CDS             complement(4509..5468)
                     /locus_tag="PK576_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE61768.1"
                     /translation="MPSPRYLFGPVTSRRYGRSLGVDLAAPKTCTLNCRFCQLGPTSA
                     TTVTRTDSPPIGEVIGELRVWLASGQTAAFITASGSGEPTLHRRFGDLLRFARDETPC
                     RSLLLSNGTLFGLPEVRREAAQADVVKLSLHAWDQASFEQITRPHPSLRFETILNGYR
                     AFRQEFGGQIDLEVFLIHGVNDSPEQAQRIAALARQFGPDTLTLNTAVRPPADSGVTA
                     CPQDRMRALAPEFGPDTRISGAEPPTSPVAFTTEALASLVSRHPVSLNSLAATFALTE
                     EEMSARLAPLVAQRAIRLFETRGVVFAGPPVSEDQEPDDGPPQ"
     gene            complement(5677..>6408)
                     /locus_tag="PK576_09005"
     CDS             complement(5677..>6408)
                     /locus_tag="PK576_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006004953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOE61769.1"
                     /translation="NTFPTAVLCRPFDFGADIVVYSTSKYLDGHAVALGGMIVDSGRF
                     DWRAGGKFPGLTEPDPSYHGIVYADAFGPSAFIVKARVQLMRDLGAAPSPTNAFLLNL
                     GMETLHLRMERHCRNAEAVAAFLEKHPAVAWINYPGLPSSRDAALVRKYLPRGTCGVI
                     SFGVRGGRDAAMRFMDSLKLAAIVVHVADVRTSVLHPASTTHRQLTDAQLTATGIRPE
                     MIRLSVGIEHPDDLIADIEQALAVC"
BASE COUNT         1136 a         1860 c         2117 g         1295 t
ORIGIN      
        1 tctttggttt ttttctgaca tcttcgaggc acagctcaat gcaccgctcg cagttctgcg
       61 gctcgaccgc cgcctcttcg cagcggtacc cgaaaccgcg cacccacttc gcgcccgccc
      121 tggggcaggt cttgacgaaa tccgcaaact cggtgaagcg ctccacaatg tgcgacgaca
      181 cgccgtgctc catatggcag gcagcctgat cagcctccgc ctcgtcgatc gagagcacat
      241 gcaccatgaa gtcacgcaac gcctcatgcc gccgcacgac gcggctggcg accttcttcc
      301 cctcatcggt cagcgtgaca aaaccgtagc gctcataatt caccagcccg cggtcgcgca
      361 aggcttgcaa catcccggtg acagacgacc ggttcacctt gaggcgttcc gcgatatcct
      421 tcgaccgggc aaccgtattc tcccggacaa ggtaaaagat cgcctccaaa taatcctcga
      481 tactgatact caacgcttct gtcatcgcca tgtctctcgg tcccttcttt ctgagagttc
      541 gtggagacaa acatactacg gacatccgta catgtcaagg ggtcaacttg cttttttggc
      601 tacgaccttc tcaaggtgcg gtcggctccg ctcccggctt gtgcgcctgc acatccacca
      661 tgttcccacc ggtacgcagc agaacggtgc tgctgaaagc atcaccctcg cgcgtggctg
      721 ccacgccgtt gatccgcacg tcgctgtcag gatcggccgt gccgcggata tcgacttcga
      781 cgcctgccgc cgtgacgcgc ccgaggcgtg gatgcaacac cgcgccgtta atcgatcgcg
      841 tcggtcacct gcacgaccgg tttccacgct tctttaaatg tgtcgagcgg ccagagttca
      901 aaccgccgca cgatcatttc ggcaccttcg gtctgaatgc aaacacgccc ggcactgggt
      961 ttcaccgcga aggcctcatt cacgagacga ccgttaaaga agtaccggag cgtgtcgccg
     1021 cgcgcgatca cttcgagccg gttccagtcg cctaccggtg cgtcgaggtc atacagaccg
     1081 cggaaaccga gcaggtcctt ccaatcctca tcccgctttt cccagttcac gcgaccgccc
     1141 gtcaaggttt ggcgcggagc gcctttcttc cagcgttttt caccgtcgcg atccaactcg
     1201 atttcagcct caatgctggt cgtgaccggc gtgccgtccg gctgcttgcc actgaccaca
     1261 atgatgtcgc ccatgccgcc ttcgatgatc tgcgcctcca cacttgcgat ccagaccccg
     1321 ccgcaactgc cgtgcgggcc gtgcgcgtgc accagaatgc cgttatcgcg cgccttgtcg
     1381 acgcgtttgc cgcaggtggc tccggtccac ctgaactcaa cgacaagatg gtaatcctga
     1441 taggcttgct gcgtcgcgag gtaaccgtag ccgcgtccgc tgatgtgcag catcccgtcc
     1501 tcacggacct gaaagatctc gctgggctta tctgagaatt cggacttcgg ccccagatgc
     1561 acctcagtct gcttttgccg gatcaagtcg atgacatcga ccttttcggt caccggtgcc
     1621 gcctgtgtcg ccagcgccag ccatccggcg accgctgcac caccccacca tgccccatgc
     1681 cctcttgtca ttttttcacc tcacgtattt gaaaaacaaa ctcagggtgt caacagtata
     1741 acacagcatg ccttatcgtc acgaaccgat tccgtcatcg ggcgggattg ccccgtttgg
     1801 aaagatatgc gacactgtat cacgtttccg gcggattgaa tgagtctttt ccggcgggtt
     1861 gagggtgaga gagatgaaag cgttggcgaa atattcaatg gggatcggtg atcggttcgg
     1921 gcgtcagggc gaggcgcagc ttgaggcgtt caagcggttc cgcgagcaat gcggggtgga
     1981 ggtggtgccg gtctggaaca aatccgatcg ggagcacaag acgatcggca cccggccgga
     2041 gtcggtgcgt caggaggcgg atgcggcggc ggcgaaagtg gggtggacgg caccgtatca
     2101 tgtggatgcg gatcatatcc gtctcgccac ggtcgacggc tttatcgcgt cttccgactt
     2161 tttcactctg gatgtggccg attacatggg agagacgacc gatgaggcgg agatctacgg
     2221 gtttgtggcg cggcacgagg aactgatcgg gtcgcatgcc atcgaggggc tggagtcgcc
     2281 cgtgaccctg accaagccgc agatggcgga gattgtgcgc ggtacgttgt ttgcgatccg
     2341 tcaggcggga cggctctacc ggcacattgc ggaacaaaaa ggctcggagc cgttcatcac
     2401 agagatctcg atggacgaga caacgcagcc gcagactccc gagatgctgc tggtcatttt
     2461 ggcggtggtc gctgaagagc aaattccggc gcagacgatc gcgccgaaat tcaccgggcg
     2521 gttcaacaag ggggtggatt atgccggtga cgcggcgttg ttcggccgcg agtttgatgc
     2581 ggatgtgtgt gtcgtgcggt atgcggttca gcatttcggt ctgccccgga atctgaaact
     2641 cagtatccat tccggcagcg acaagttttc gctctacccg tatgtggccc gaacgcttca
     2701 ggcgcgcgat gccggggtgc atgtgaagac ggcgggtacc acttggctgg aagaggtcac
     2761 ggggctggcc gtgtccggcg gcgaggggct ggcgatcgcc aaggaaatct acctgaaagc
     2821 cctgccgcgc tttgatgagt tgtgcggccc ctacgcaacg gtgctggaca tctctccgga
     2881 acggttgccc tcgccggatg aggtggcgtc ctggaacggc ggacggttcg cggcctcgtt
     2941 gcggaatgtg ccgggcgacg cgcacttcaa tccggacttc cgccaattca tccatgtggc
     3001 ctacaaagtg gctgctgaat tgggggagcg gtacttgacg gcgttggatg aaaaccgcga
     3061 aaccgttgcg cgtcatgtta cggaaaacct gctgacaagg cacctgaaac cgatcttccg
     3121 cggcgcgtga gcgcggtata gggacggaaa cagggtggtt atcccgaagc attcctatac
     3181 ttcatggagg tgaatgatgc acacagcgat tgtcgccgaa cggggacagg taaccattcc
     3241 aaaagctttg cgtgaccgga tgggtgtccg gccctccacc gttttgtcgt tcagcgtaaa
     3301 agacggcgct ctgatcgccg tgaaagcggt gaaagaggat ccggtctctt acgttcgggg
     3361 ggatgaatga cgcaggaaac ggaagacccg cgactcgcgg cgattttcac gagtttcgcg
     3421 catgtgggcg cgttcctgtt cgggggcgga tatgcgatgc ttccgttgat cgagcgggaa
     3481 gtggtcaacc ggcgcgggtg gtgccgcttt gaggagatgg cggattatta cgcgatggct
     3541 cagttggtgc ccggggtgat cgcggtgaac acggcgatgc tgatcggaca ccgtctgcgt
     3601 ggattccgcg gggccgcggc ggcgatgctc ggtgtggtgt gcgcgccctt ttttgtgatt
     3661 ctggcgtatg cggtggctgt ggaccggttg gcggggatgc cgtttctgga gcgtgccttg
     3721 gcggggttgc ggcccgctgt ggcggggatg atgctgggta tcgcgtacac gatgttcaac
     3781 cggacccgcc ggacgcggtt ggggttgacg gtgtgtctgg tgaccacggc gctggtcttg
     3841 ggggccggag tgagcgcggt ggccgtcatc ctgttgggcg tcacggccgg gctggtcgga
     3901 tacggggtgc ggaaggggtt gcgcggatcc gtttctgatg cggaaaacag cggggggcag
     3961 ccgtgaacct gctgctcaaa ctcgcttcgg tcttttttaa gatcggtctg ttgaccgtgg
     4021 ggggcgggct ggcgatgatt ccgatcgtcc agcaggagat ggttggacgg gggtggctga
     4081 ctaaccgcca gtttctggac attctcggca tcgcgcaaat gacacctggc cccatgtcgg
     4141 tcaacacggc gacgtttgtc gggtatcgtg tggtcggtac cgaatatccc ggcgcggtct
     4201 ggctggcggt ggccggatcc gtggtcgcaa cgctggcggt gtgcgcccct tcacttctct
     4261 gcatcaattt gctcggggcc ttctggatgc ggaaccggga acacccgtgt cttgtgagtg
     4321 ttttcacctt cctgcgtccg ctggtgaccg gtctggtgat ccacgccgct ctgttgttgg
     4381 gggtgaactg cctctgggcc gatccggtcc gcgaatggtt gtctcagtca ccggatgtcc
     4441 gcgctggcgt gttgctggtc gtggcgtttc tcctgaccgt tctgacccgc gtttcgccgg
     4501 ttttggttct actggggggg gccatcgtcg ggctcctgat cttctgatac cgggggcccg
     4561 gcgaagacca cgccgcgggt ttcaaacagg cggatggcgc gttgcgcgac gagcggggcc
     4621 aagcgcgcgc tcatctcctc ttccgtcagc gcgaacgtcg cggccagaga attcagcgaa
     4681 accggatggc ggctgaccag ggacgcgagc gcctctgtgg tgaacgccac aggggaagtg
     4741 ggcggctctg cgccgctgat ccgggtgtcc ggcccgaatt ccggcgcgag cgcgcgcata
     4801 cggtcttgcg gacaggcggt gacgccggag tcagcgggcg gccgcacggc cgtgttgagt
     4861 gtcagggtat ccggaccgaa ctgccgggcg agagcggcga tccgttgcgc ctgttcgggg
     4921 ctgtcgttca ccccgtggat gagaaaaacc tcgagatcaa tttgcccgcc gaactcctga
     4981 cggaacgccc ggtatccgtt gagaatcgtt tcgaaccgga gtgacggatg cggtcgcgtg
     5041 atctgttcaa aggaggcttg atcccacgcg tgcagggaga gtttgaccac atccgcctga
     5101 gccgcttcgc gccggacctc cggcaggcca aacagggttc cgttggaaag cagcagagag
     5161 cggcagggcg tctcgtcgcg cgcgaaacgc agcagatcgc cgaacctgcg gtgcagagtg
     5221 ggttcgcctg aaccgctggc cgtgatgaac gccgcggtct gtcccgacgc caaccagacg
     5281 cgcaactccc cgatcacctc gccgatgggc ggggaatcgg tgcgggtcac tgtcgtggcg
     5341 gatgtggggc cgagttggca gaaccggcag ttgagggtgc aggtcttggg tgcggcgagg
     5401 tcgacaccca acgaacggcc gtaacgccgg gaggtgacag gaccgaaaag ataacgggga
     5461 gagggcatgt cagtaccata atctggccat tctgtcacac gaaggccgta ttgaaaaagg
     5521 cggggtggtt tccccgaaac cgctgcggac gccttgggga agcgtcccta ccgttggcgt
     5581 aacgagaaat tcaacgttcc ttgtccgacg ttcagcgttc caattatcct cacattgtac
     5641 attgtcgccc gggtgcggca tcgtgatcgc tcaccttcag cagactgcca gggcctgctc
     5701 gatatccgcg atgaggtcat ccggatgttc gatgccgacc gagagccgga tcatttccgg
     5761 gcggatgccg gttgcggtga gttgcgcgtc cgtgagttgg cggtgggtgg tgctggcagg
     5821 gtgcagcacg ctggtgcgga cgtcggcgac atgcacgacg atcgcggcga gcttgaggct
     5881 gtccatgaag cgcatggcgg catcccgtcc gcccctgacg ccgaaggaga tcacgccgca
     5941 agtgccgcgc ggcaggtatt tcctgacgag ggcggcatcc cttgagctcg gcagaccggg
     6001 gtagttgatc caggcgaccg cggggtgctt ctcgagaaag gcggccacgg cctcggcgtt
     6061 gcggcagtgg cgctccatgc gcaggtgcaa cgtctccatg ccgaggttga gcagaaaggc
     6121 gttcgtcggc gagggggccg cgccgaggtc gcgcatgagc tgcacgcggg ccttgacgat
     6181 aaaggcggac gggccgaagg cgtcggcata gacaatgccg tggtaggagg ggtcgggctc
     6241 ggtgagcccg gggaacttgc cgcccgcacg ccagtcgaag cgcccgctgt ccacaatcat
     6301 gccgcccaac gcgacagcat ggccatcgag atatttggag gtggagtaga caacgatgtc
     6361 cgcgccgaag tcgaaggggc ggcagagcac ggcggtcggg aaggtgtt
//
LOCUS       DAOSLE010000147         6397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_10988_length_6397_cov_8.740303, whole genome shotgun sequence.
ACCESSION   DAOSLE010000147 DAOSLE010000000
VERSION     DAOSLE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10988_length_6397_cov_8.740303"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="PK576_09010"
     CDS             <1..2025
                     /locus_tag="PK576_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61770.1"
                     /translation="GVTADGAGLVSRWADQSGNGHEAVRGDSRYQPCLTNNVVNGLPG
                     LRFEGDTYKDGLITTWGTSNGTYSVFVTASYQVSTTYSWYRLATGPANYNWGIELNGS
                     GGDFYSFIPNGAGGNEYYQIVNTGIRPAGGVPFTAAILGDGTNTQFTLNGFGFTPIAK
                     HHGPGTLILGHGGISGNPWLGHIFEVIVYSRALGFEERRQVERYLALKYGIRSAHLTP
                     SAGLVQWLRAENAIADVADGAANRVKRWDNQTQYGSGVQATQATTNRMPEKIAGALNG
                     KPVLRFDAAAGEYDSLKAVLASVDKTYSVIAVFAPRSADPAERIVLQGVNGASRLSVT
                     NGLVTRQASGTVSQLAPAQVNRAAIAVMTCDAQASRFYLNGVNLTQNSAPVGLWKGTG
                     SSVIYLGAGTGDGALDLPLDGDLAEVLVYDRLISDTERRRIEADLSERYAIPVARVNP
                     AVWSSEFGGVWHFTGTDRLLLADASAAQNGAALLGQAAPAAAAALAGEGLAWDAAAQA
                     GRTRAQPAAVPQTFSFWAKQQAPVADQAVVLAGTGAVAYAALDNAAEKLEVAGTDAAD
                     LTAPNAAGWTHYAVTVNPSGSVQAYGNGEPLTTVPNALAALLPVNQALTLGHAGDETD
                     NTKTFSGVLDEVRIETVPRGADWIRAAYMTQAENESFTSYNRWGTLMLMR"
     gene            2220..3245
                     /locus_tag="PK576_09015"
     CDS             2220..3245
                     /locus_tag="PK576_09015"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOE61771.1"
                     /translation="MKTPLPDTLPSDTFCLAPLRGVTVRAFRALHARWFTPPDYAVAP
                     FIPTFAGGKVKRTLLKDIDPALGQAVPVVPQVIGKEPLLLRTLLQVFKSMGYDGADLN
                     AGCPYPFIVKKGRGAGLMRDAGAFARMLEAGCEEMPQGFSVKVRLGIDRPDLLMERME
                     VINAFPLREVTIHARTARQMYAGRVDLAAFEEARRACRHPVVYNGDLCTRADFLLLKT
                     RFPMITRWMIGRGAAVDPFVFGRLTAEASPPRDAERLKRFLDEYLDACLEELHGPASV
                     LGRLKELWGYLHRSLAGGGRVWKRVRVCRTVDEYRRVVEESFGRAFPGFVEETERNGF
                     GPADRGD"
     gene            3281..6274
                     /locus_tag="PK576_09020"
     CDS             3281..6274
                     /locus_tag="PK576_09020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOE61772.1"
                     /translation="MLILAALLPCGGILHAKNAVTEAYALKLWTVEDRLPGSPVVGVS
                     QASDGYLWLVTGAQLIRFNGVHFTEIAVPEEVRRQTGGFGGVFCGRAKGVWVYGPSGV
                     ACCRAGQWQVWSGAAVSGTAGRILSMVETPDGVVRAVAERGLLEAVAAADDLATQFVA
                     QLCPVPPDDRATLGAVTDADADGSGSIWMTAWNGLVEYRAGRYDDQSMRLPDFLVEAA
                     DGIHAGRSGRLWIHGSNGVAYREKSIWTPIGFPENAGMATVMLEASDGSLWIGNPTGM
                     FRWDAGVWRRLAEQDTPGSLSVNTLIEDRDGTLWAACDGGLLRIRKKSVEPLRAEGTA
                     AMGTAYGLWRTPEGGAWVGFKGRAARLTAREWRLLQTVYLDADVPVSALLQDSAGRVW
                     LGTLGGGLFLYQQGSLTLVPQRDYSMPAAHTVYTLMEDAEHGILAGTPQGLMRVNVAG
                     ELEQAVIRGALFAEPVYALYRDRDGALWICSEGTGLVCLRRDGAREGIGHEAGLEGYP
                     RVTYRDTRGQFWVGTTAGLFLLTPQALIPLGRQTGLFGDAVLQLSEDEAGRLWLGTTR
                     GLLGVVIEDLARLAQAGGKDETVAVRLLHLGVSDGMPGERCMGGVLSRGGAETGRLWF
                     PCENGVAVVDPQKVGFSGQPPTVVIERVLVNGQEVLNNVAGEPRTAVFAPGARNVVFH
                     FAALSPGAADSARYRYRVDGLRMAWSPVQAEQQAVFEWLPPGRYTLKVMAEAGGIWNL
                     NGAALAFEVRAYFWQTTWFYLVLALALAATVFLVARWILWHRYQLQMAILKREEALAQ
                     ERARISRDIHDDLGNGLSVVATLSELAHNDVEKGLVHKRLDQIYEVANELARNVDETV
                     WAVNPANDGWEPFLSYFEQYTEYFLGNSGLRFHFTRPAELVERPIASKTRHHLLLAVR
                     EAVGNVLKHASAKQVRIAMRLDGVVLEVRVEDDGVGFDPSACAGVGHDGLGNMARRMR
                     EAGGVMTLDSAPGAGTRVLFRVPLE"
BASE COUNT         1020 a         1852 c         2398 g         1127 t
ORIGIN      
        1 ggcgtgacgg ccgacggcgc cgggctggtc tcgcgctggg ccgaccagtc gggcaacggg
       61 cacgaggccg tgcggggcga ttcccggtac cagccctgcc tgaccaacaa cgtggtcaac
      121 gggctgcccg gcctgcgctt cgagggggac acctacaaag acgggttgat cacgacctgg
      181 ggcaccagca acggcaccta ttcggtgttc gtaaccgcca gttatcaggt gagcacgacc
      241 tatagttggt accggctggc gaccggcccg gccaattaca actgggggat cgagctgaac
      301 gggtcggggg gggatttcta ctccttcatc cccaacggtg cgggcggaaa cgagtattat
      361 cagatcgtga acacgggtat ccggcccgcg ggcggcgtgc cgttcacggc ggcgattttg
      421 ggcgacggca cgaacacgca gttcacgctg aacggcttcg ggttcacgcc gatcgccaag
      481 caccacggac ccggcacgct gattctggga catggcggca tcagcggcaa cccgtggctg
      541 ggccatattt ttgaggtcat cgtctacagc cgcgcgctgg ggttcgagga gcggcggcag
      601 gtggagcgct acctcgcgct aaagtacggg atacgctccg cccacctcac gccctccgcg
      661 gggctggtcc agtggctgcg cgccgaaaac gcgatcgccg acgtcgccga cggcgcggca
      721 aaccgtgtca agcgatggga caaccagacg cagtacggga gcggcgtgca ggccacgcag
      781 gccacgacca accgcatgcc cgagaagatc gccggggcgc tcaacggcaa gccggtgctg
      841 cggttcgacg ccgcagcggg cgagtacgac agtctgaagg ccgtcctggc gtcggtggac
      901 aagacctact ccgtgatcgc ggttttcgcc ccgcgttccg ctgaccccgc cgagcggatc
      961 gtgctgcagg gcgtgaacgg ggccagccgc ctgagcgtca ccaacgggct ggtgacccgg
     1021 caggcgtcgg gcacggtctc gcagctcgcg cccgcgcagg tcaaccgcgc cgccatcgcg
     1081 gtcatgacct gtgacgcgca ggcctcgcgt ttctacctca acggcgtcaa cctgacccag
     1141 aacagcgcgc cggtcggact ttggaaaggc acgggcagca gcgtcatcta tctcggcgcg
     1201 gggaccggcg acggcgcgct cgatctgccg ctggacggcg acctcgccga ggtgctggtg
     1261 tatgaccggc tgatctcgga caccgagcgc cgccgcatcg aggctgacct ctccgaacgc
     1321 tacgcgatcc ccgtggcgcg ggtgaacccc gcggtgtggt catccgaatt cggcggcgtg
     1381 tggcacttca ccggcacgga ccgcctgctg ctggccgacg cctcggcggc gcagaacggc
     1441 gcggcgctgc tcgggcaggc cgcgcccgcg gcggccgcgg cgctggcggg cgaggggctg
     1501 gcgtgggacg ccgcggcgca ggcgggccgg acgcgcgcgc agcccgcggc cgtgccgcag
     1561 accttctcgt tctgggccaa acagcaagcg ccggtcgcgg atcaggcggt cgtgctggca
     1621 ggcacgggcg cggtggcgta cgccgcattg gacaacgcgg cggaaaagct ggaggtcgcg
     1681 ggtacggacg cggccgatct gacggcacca aatgcggcgg gctggaccca ttacgccgtg
     1741 acggtcaatc cctcggggag cgtgcaggcc tatggcaacg gagagccgct gaccacggtg
     1801 cccaacgcgc tggccgcact tctgcccgtc aatcaggcgc tcaccttggg tcatgcgggc
     1861 gacgagacgg acaacacgaa gaccttctcc ggcgtgttgg atgaggtgcg cattgagacc
     1921 gtgccgcgcg gtgcggactg gatccgtgcc gcctacatga cccaggcgga aaacgagagc
     1981 tttaccagct acaaccgctg gggaaccttg atgctgatgc gctgaacacg ccatgatgcc
     2041 ttgcgccgag tatcctcggg ccttcaaacg ttcggcaaaa gcccggttcc ccatggtcag
     2101 aaagtagaag gccagcatgc ggtccgcact ggaaagggag cgttcccgcc cgcaatggcg
     2161 gatcagccgg acaacccaga cgccgcaagg cgaatgtcgc gggtcgtagg tcgacgcaca
     2221 tgaaaacgcc gttgccagac acgcttccta gcgatacgtt ctgccttgcg ccgctgcgcg
     2281 gcgtcacggt gcgggccttc cgtgcgttgc acgcgcgctg gttcaccccg ccggactacg
     2341 cggtcgcgcc gttcatccca acctttgcag gcgggaaggt caaacggacg ctgcttaagg
     2401 atatcgatcc cgccctggga caggcggtgc cggtggtgcc gcaggtgatc ggcaaggagc
     2461 cgctcttgtt gcgtacgctg ttgcaggtgt tcaagtccat gggctatgac ggtgcggacc
     2521 tcaatgcggg ctgcccgtat ccgttcatcg tgaaaaaagg ccgcggcgcg ggactgatgc
     2581 gggatgccgg ggccttcgcg cgtatgctgg aggcgggctg cgaggagatg ccgcaggggt
     2641 tttcggtaaa agtccggttg ggcatcgacc ggccggatct gctgatggag aggatggagg
     2701 tgatcaacgc ctttcccttg cgcgaggtga cgattcatgc gcgcaccgcg cggcagatgt
     2761 atgcgggccg cgtggacttg gcggcgttcg aggaggcgcg gcgggcgtgc cggcatccgg
     2821 tcgtgtataa tggcgatctg tgcacgcgcg ccgattttct tttgctcaag acgcggttcc
     2881 cgatgatcac gcgctggatg atcggccgcg gggccgcggt cgatccgttt gttttcggcc
     2941 gcctcaccgc agaggcgtcg ccgccccgcg atgcggagcg gctcaagagg tttctcgacg
     3001 agtatctgga cgcgtgtctc gaggagctgc acggaccggc atcggtgctc gggcgtctga
     3061 aagagttgtg gggatatctg caccggtcac tcgccggagg cgggcgcgtc tggaaacggg
     3121 tgcgggtctg ccgcacggtg gatgagtatc gacgggtggt ggaggagtct ttcggccggg
     3181 cctttccggg gtttgtcgag gagacggaac gaaacgggtt cggcccggcg gacaggggag
     3241 attgaaggcg gatatgcgcg gtcacttcag aattctatgg atgctgatcc ttgcggcgct
     3301 gctgccgtgc ggcgggatcc tccacgccaa gaacgcggtg accgaagcgt acgcgctgaa
     3361 actctggacc gtggaggacc ggttgccggg ttcaccggtg gtcggtgtgt cgcaggcgtc
     3421 ggacgggtat ctctggctgg tgaccggtgc gcaactgatc cggttcaacg gtgtgcattt
     3481 tacggagata gcggttccgg aggaggtgcg gcggcagacc ggcgggttcg gcggggtttt
     3541 ctgcggccgc gccaagggag tttgggtgta cggtccctcc ggggtggcct gctgccgtgc
     3601 gggccagtgg caggtgtggt ccggcgcggc ggtttccggt acggcgggac gtatcctgag
     3661 catggttgag acgccggacg gggtggtgcg cgcggttgcg gaacggggtc tgcttgaagc
     3721 ggtggctgcg gcggatgatc tcgccacgca attcgtggcg caactgtgtc cggtgccgcc
     3781 cgatgaccgc gcgaccctgg gcgcggtgac ggatgcggat gcggacggtt cgggcagcat
     3841 ctggatgacc gcgtggaacg gactggtgga gtaccgtgcg ggccggtacg atgatcaaag
     3901 catgcggctg ccggattttc tggtggaggc ggccgacggg attcacgcgg ggcgttcggg
     3961 gcgtttgtgg atacacgggt cgaacggggt cgcgtaccgc gagaagagca tctggacccc
     4021 gatcgggttc cccgaaaacg cggggatggc cacggtcatg ctggaggcgt cggacggttc
     4081 gctctggatc ggcaacccga cgggcatgtt ccgctgggat gccggagttt ggcggcggct
     4141 ggctgaacag gacacgcccg gaagcctctc tgtcaacacg ctgattgagg atcgggacgg
     4201 caccttgtgg gccgcctgcg acggcggatt gctgcgcatc cggaagaaga gcgtggagcc
     4261 gttgcgggcg gagggtacgg ctgcaatggg cacggcctac gggttgtggc gcacacctga
     4321 ggggggcgcc tgggtcgggt tcaaagggcg cgccgcgagg ctgacggcgc gtgagtggcg
     4381 tttgctccag accgtttatc tggatgcgga cgtgccggtg agcgccttgc tgcaagacag
     4441 cgcgggccgc gtctggctcg gcacgctggg cggtgggctc ttcctgtatc agcagggcag
     4501 tctcacgctg gtgccgcagc gcgattacag catgccggcg gcacatacgg tgtacacgct
     4561 gatggaggat gcggaacacg gtattctggc cggtacgccg caggggctga tgcgggtcaa
     4621 tgtagccggg gagttggagc aggccgtgat ccgcggcgcg ctgttcgcgg aaccggtgta
     4681 tgccctgtac cgcgatcggg acggcgcgtt gtggatttgc agcgagggca cgggactggt
     4741 ttgcctccgc cgcgacggcg cccgcgaggg gatcggacac gaggccgggc tggaggggta
     4801 tccgcgggtg acctaccgcg acacgcgggg gcagttctgg gtggggacca cggcagggct
     4861 ttttctgctc accccgcagg cgctgatccc gttggggcgt caaaccggtc tgttcggcga
     4921 cgcggtgttg caactctcgg aagacgaggc cggtcggctc tggctgggca ccacgcgcgg
     4981 tctgctcggc gtggtgatcg aggatctggc ccggttggcg caggccggcg gaaaggacga
     5041 gaccgtggcg gtccggttac tgcacttggg cgtttcggac gggatgcccg gcgagcgctg
     5101 tatgggcggg gtgttgtcgc ggggcggcgc ggaaaccggt cgcctctggt ttccttgtga
     5161 gaacggggtt gcggtggtgg atccgcagaa agtcgggttt tccgggcagc cgcccacagt
     5221 ggtgatcgag cgggtgttgg tgaacggtca ggaggttctg aacaatgtgg cgggcgaacc
     5281 gcggacagcg gttttcgcgc ccggcgcgcg caatgtggtg tttcatttcg cggccctctc
     5341 gccgggagcg gcagactctg cgcgttaccg ctaccgggtt gacggactcc gcatggcgtg
     5401 gtcgccggtg caggctgagc aacaggcggt gttcgagtgg ttgccgcccg gacgttacac
     5461 cctgaaggtt atggccgagg cgggcggcat ttggaatctg aatggggcgg cgctggcgtt
     5521 tgaggtccgg gcgtatttct ggcagaccac gtggttttat ctggttctgg cgctggcgtt
     5581 ggctgcaacg gtctttctgg tcgcgcgctg gatcctgtgg caccggtacc agttgcagat
     5641 ggcgattctc aaacgtgagg aggcgctggc gcaggagcgc gcgcgcattt cgcgcgacat
     5701 ccacgacgat ctcggcaacg ggctttcagt ggtggccacg cttagcgagc tggcgcacaa
     5761 cgatgtcgag aaaggattgg tccacaaacg gctggatcag atttatgagg tggctaacga
     5821 gctggcccgc aatgtggatg agacggtttg ggcggtgaat cccgccaatg acggatggga
     5881 gccgttcctc agttatttcg agcagtacac cgagtatttt ctggggaatt ccgggctgcg
     5941 gttccacttc acgcggcctg cggaactggt ggagcgtccg atcgcctcca aaacccggca
     6001 ccatctgctg ctcgccgtgc gcgaagcggt cgggaatgtg ctcaaacacg cctccgcaaa
     6061 acaagtgcgg atcgcgatgc gtctggacgg cgtcgtgctg gaagtgcgtg tggaggacga
     6121 cggggtcggc tttgacccgt cggcgtgcgc gggagtgggg cacgacgggt tggggaacat
     6181 ggcgcggcgc atgcgcgagg cgggaggggt catgaccttg gatagcgcgc cgggcgcggg
     6241 tacgcgcgtg ctgttccgtg tgccgttgga atgaggggat tcgtgccaaa gccaggggtg
     6301 gaagggtatg ggcagaaggt cagcgccacg ttgacccttc acactaaaag gagcagttga
     6361 actttcaacg ctcttcggtt tctggtttca gggtttc
//
LOCUS       DAOSLE010000148         6332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11126_length_6332_cov_9.207105, whole genome shotgun sequence.
ACCESSION   DAOSLE010000148 DAOSLE010000000
VERSION     DAOSLE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6332
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11126_length_6332_cov_9.207105"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..1854)
                     /locus_tag="PK576_09025"
     CDS             complement(541..1854)
                     /locus_tag="PK576_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61773.1"
                     /translation="MQNRREFMKGSAVFGAMMAAAPSVVVGQGAARVFKVGMIGAGGR
                     CSGAMANLVEAAKNLGHEVKLVAVCDFFEDRARNAAKKFGCEEKNVFWDANSYKKVVA
                     SDCEIIVTATPPNFRPVHVEAAVKAGKHVFAEKPVAVDGPGVRQFLAAAKLAKEKNLA
                     LVAGTQRRHQKGYLLQALALQQGKIGQVLGGAVYWNGTVPWVRARKEGMRNDAYLCSN
                     WVNWTEMSGDHIVEQHVHNIDVANWFLGRYPRTAGAFGARMRRVSGNQYDFFGVDFDY
                     GDGIHIHSQCRQIGGTSGFVGERFRTEQFEISGGGRIQKVGGDALKLEGTEYTLNGNT
                     YRIKDGNPYVLEHENLLLGLLGQIPIMNEGESVAMSTACAVIGRISAYTGQTVRMSDI
                     IENANSPFYNLACKPTWQEFEAGEVAMPEYGDDQAPLPGKPWGQK"
     gene            complement(2040..3005)
                     /locus_tag="PK576_09030"
     CDS             complement(2040..3005)
                     /locus_tag="PK576_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOE61774.1"
                     /translation="MKRREFLTTSALVGAGSVCVTSHAAAADACCPASAMPKLTPPKK
                     PAQLNLALQWGAIPGSEIAQKLDFLEANGFAAVEIPSGDWPIKNCDAMIEAMKGRKLF
                     IATACGPSNFAYAEPEKREAEVQKFLPVIEALGKMKSVGLIICPARGKLSEPGWLTAK
                     ELRKDFVENTGKRLAEHAHKHGTSIVLEPLRRNETPFLRQVADGARMAADIGPGATVM
                     GDFWHMGLEETSFMGAFICAGDLLTHVHIAGLKERIIPGVHPDADNYVDGFKGLKLIG
                     YRGAVSFEGGWPKNPADPKKGIPQEEKLRLIRNMVKMLREQWDMA"
     gene            3235..3465
                     /gene="rpmB"
                     /locus_tag="PK576_09035"
     CDS             3235..3465
                     /gene="rpmB"
                     /locus_tag="PK576_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE61775.1"
                     /translation="MSRVCEICGKGPMAGNSIIRHGLAKYKGGIGLHTTGISKRRFLP
                     NLHMVRVKSENGTVGRKKVCAACIKQGKIVKP"
     gene            3538..4059
                     /locus_tag="PK576_09040"
     CDS             3538..4059
                     /locus_tag="PK576_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61776.1"
                     /translation="MQENPADPNVPDANADVLNNPNPENALAQNEEADDEIRATDIVF
                     DCPRCGHNLAIDYRGAGLQIKCVECGEPTLVPIPDGMKIDDLDLSPGELLTQLFQTRR
                     MLLKSEQQISELEETLNSMKLRRTELEKARMNTLRWCAEVVNLCQSALKSQNETALTL
                     NRVIALIGEEQQR"
     gene            complement(4193..4969)
                     /locus_tag="PK576_09045"
     CDS             complement(4193..4969)
                     /locus_tag="PK576_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOE61777.1"
                     /translation="MKQTDEQLWREARERLLALDTVWPDAPVRRRSLEACRAWLAARH
                     EQPRLSALARLLTPDMTRAQFWCALVPVEREVARIRVTDIEILDRDLTPESEPAAPLM
                     PLTVVTDSLRSAFNVGGIFRTAECFGVAEVVLCGYTPAPDRPQTARAALGAEKLVRWR
                     DGGDVRAALAGLKANGVRCVALETVADAKPLERAEWAFPCALVLGNERFGLDADVIKL
                     CDEVVRIPLCGRKNSLNVVSAFTIAAYTARQWFDMKSKTG"
     gene            5113..6282
                     /locus_tag="PK576_09050"
     CDS             5113..6282
                     /locus_tag="PK576_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61778.1"
                     /translation="MKQRMMMAAASLTASAVLAGLYGDTPDARHAWAVHDRNRPNPVK
                     ITAEPGMPPSDATVLFDGSSLEAWESTKGEPTKWRLVDGVLESVKGAGAIRTKQSFGD
                     CQLHLEWAAPTRVEGMGQGRGNSGVFLMSSYEIQILDSYETYTLPDGSNKNPNYADGQ
                     AASVYAENPPLVNACREPGAWQTYDIIFHQPIWEGDKLKYPGSLTVLHNGVLVQDHWE
                     MEGLTTHCRRRPLAPHATKLPLQLQDHGNPVRFRNIWIREIPSRYANTTHGGPAANEA
                     DVMALRKETAAKLFAQILPENPRKAEALHQILEVISYEKAEPYTALAKKLCDAYLNEL
                     AGCGAQQLESRKGEIIGLRNACNVLIRNKIYDERCPLHATLQKIITEQGFEKKKK"
BASE COUNT         1305 a         2014 c         1805 g         1208 t
ORIGIN      
        1 cttcaactct ctttggtttc agggtttcag ggtttcaggg tttcagggtt tcatcgggat
       61 cgggatcgct atcggtatcg gtatcggtat cgggatcggc gacccgcgac ccgcgacccg
      121 cgacattcga cccgcgacga gcgacaggga cgcccgcgtt tccgacaccg caggtcgctc
      181 gtcgcgggtc gacatataag gtctgacggg agaagcgggc gttccgcccg ctggcaggga
      241 cggttccccg aaccgtccgc ggacgcctcg gggaggcatc cctaccggtt ggcggacgcc
      301 ttcgtcccgc atacgcaccg gtctccaaaa ctcagtcgat tgcgcttgcg acgaagtgga
      361 gcgatgaagt gccgggacga agagttcaaa aagagcgccg gacgcaccgt tgcgttttgc
      421 accaaacagg tgcggcgggg accgcgacaa aacccaatgt ttgcgacagg ccatttgggg
      481 ggtaaaaaca aacgcccgtc cggcaaaccg gacgggcgtt gcgatacagc ggggcacccg
      541 ttacttctgt ccccagggct tgcccggcag cggtgcctga tcgtcaccgt attccggcat
      601 cgccacctcg ccggcctcaa actcctgcca agtcggcttg caggcgaggt tgtagaaagg
      661 cgagttcgca ttttcgatga tgtccgacat gcggaccgtc tgtcccgtat aggccgagat
      721 gcggccgatg accgcgcagg ccgtggacat cgccacgctc tcgccttcgt tcatgatcgg
      781 aatctgaccg aggaggccga gcagcagatt ctcgtgttcc agcacatagg ggttaccgtc
      841 tttgatccgg tacgtgttgc cgttgagcgt atactccgtg ccctccagct tcaacgcatc
      901 cccgccgacc ttctggatcc tgccgccgcc ggagatttcg aattgctccg tgcggaaacg
      961 ctcacccaca aagccgctgg tcccgccgat ctgacggcac tggctgtgga tatggatccc
     1021 gtcgccgtaa tcgaaatcca ctccgaaaaa gtcatattga ttacccgaca cgcgacgcat
     1081 gcgtgcgccg aacgcgcccg ccgtgcgcgg ataacggccg aggaaccagt tcgcgacgtc
     1141 gatgttgtga acatgctgct caacgatatg gtcaccggac atctcggtcc agttcaccca
     1201 gttgctgcag aggtaggcat cgttacgcat cccctctttc cgcgcgcgca cccaaggcac
     1261 cgtcccgttc caatagaccg cgccgcccag cacctgcccg atcttccctt gctgcagcgc
     1321 cagcgcctgc aacagatatc ccttttggtg gcggcgctgc gtgccggcca ccagtgcgag
     1381 attcttctcc ttggccagct tggccgccgc cagaaactgg cgcacacccg gcccgtccac
     1441 ggccaccggc ttctccgcga acacgtgttt gcctgctttg accgccgctt caacgtgaac
     1501 cggccggaaa ttgggcggtg tggccgtcac gatgatttca cagtcggacg ccacaacctt
     1561 cttgtagctg ttcgcgtccc agaacacatt cttctcctcg caaccgaact tcttggccgc
     1621 attccgggcg cgatcctcga agaaatcgca cacggccacc agtttgactt catggccaag
     1681 gtttttagcc gcctccacca gattcgccat cgcgccggaa cagcgtccgc ctgcgccgat
     1741 catgccaacc ttaaacaccc gtgcggctcc ctgaccgacc acgaccgacg gcgccgccgc
     1801 catcatcgct ccgaagaccg ccgacccttt catgaactct ctacgatttt gcatctctct
     1861 ctctcctact aactcacttg cccataaatt gacaggatta caggattttc aggattaaaa
     1921 aatcatgtta atcatgtaat cttgtcataa aaaacttatc ccagagtttt ctcgcggctc
     1981 cggcgcgcgg cgggctaact cactaactca ctaatgaact aatgaactaa tgaacttctt
     2041 caagccatgt cccattgctc gcgcagcatc ttgaccatgt tacgaatcag ccggagcttc
     2101 tcctcctgcg ggataccctt tttcggatcc gccggattct tcggccaccc gccctcgaaa
     2161 ctcaccgcac cgcggtatcc gatcagcttg aggcccttga agccatccac atagttatcg
     2221 gcgtcgggat gcactcccgg gatgatccgt tccttcagcc ccgcgatatg cacgtgggtc
     2281 agcaggtcgc cagcgcagat aaaggcgccc atgaaactcg tctcctccag tcccatgtgc
     2341 cagaaatcac ccatcaccgt cgcgcccggc ccgatgtccg cagccatacg cgcgccatcc
     2401 gccacctgcc gcaggaacgg cgtctcgttg cggcgcaacg gctccagcac gatcgacgtg
     2461 ccatgcttgt gcgcatgttc cgccagacgc ttgccggtgt tctcgacaaa atccttgcgc
     2521 aactccttgg cggtcaacca tcccggctcg ctcaacttgc cgcgcgccgg gcagatgatc
     2581 aagcccaccg acttcatttt gccgagcgcc tcgatgaccg gcagaaactt ctgcacttcg
     2641 gcttcacgct tctccggttc cgcatacgca aaattgctcg ggccgcacgc cgtggcgatg
     2701 aacagcttgc gccctttcat cgcttcaatc atcgcgtcac agttcttgat cggccagtcg
     2761 cccgacggaa tctcgaccgc ggcaaagccg ttggcctcca gaaaatccag cttctgcgcg
     2821 atctcggatc ccggaatcgc gccccattgc agagccaggt taagttgcgc gggttttttg
     2881 ggcggtgtca gtttcggcat tgcgctcgcg ggacaacagg cgtccgcggc agcggcatgc
     2941 gacgtcacac atacggaacc cgctccgacc agcgccgaag tcgtcaagaa ttcgcgtcgt
     3001 ttcatacgtt tctctcccct gattcccggt tcgcaccctc ttgcccggga aacatgttga
     3061 tacaattagg gtattgggta tagaattatc atggacggct gttccgcgtc aagaagccga
     3121 ttacataaag tcgtaatcaa catgaaaaag gtcgatacga aaatcacctt tctgcttgtg
     3181 ggaacgattc cgaaatgcta tgatcaaccc cctttttgtt aaggagaaac ggtcatgtct
     3241 agagtatgtg agatttgcgg caagggaccg atggcgggga attcgatcat tcggcacggt
     3301 ttggccaaat acaaaggcgg gatcggtctg cacacgaccg gtatctccaa gcgccggttt
     3361 ctgccgaacc tccacatggt ccgcgtcaaa tccgaaaacg gtaccgtggg acgaaaaaag
     3421 gtttgcgccg cctgcatcaa gcagggtaag attgtcaaac cttgaggcca tgtgccgcgc
     3481 gtaggggttg cggcgagggt ttcgtgaaca caacggaatt atccgaaagg gggcgttatg
     3541 caagagaatc ctgcggatcc gaacgtgcct gatgcgaacg cggatgtgct gaataacccg
     3601 aatccggaaa acgcgcttgc ccaaaacgag gaggcggacg acgagatccg tgcaacggat
     3661 atcgtgttcg actgcccccg ttgcggacac aacctcgcca tcgattaccg tggcgcgggc
     3721 ttgcagatca agtgcgtgga gtgcggcgaa cccacactcg tcccgatccc ggacggaatg
     3781 aagatcgatg atctcgacct ttcgcccggg gagttgctca cgcagctctt ccagactcgg
     3841 cgtatgctgc tcaaaagtga gcagcaaatt tctgagttgg aggagacgct caacagcatg
     3901 aagctgcgcc gtacggagtt ggaaaaagcc cgtatgaaca cgctccgctg gtgcgcggaa
     3961 gtggtcaatc tctgccagag tgcgctgaag tcccaaaacg agaccgctct caccctgaac
     4021 cgggtgatcg ctctgatcgg cgaagagcaa caacgctgag gcgtcacgag cccctgattg
     4081 tcggcagcag tagcccggcc gggggtgcgc tcaccctctg cgacttgcga cggggatgct
     4141 cccgattcga ctgatccgcc gttgcgggcg gggccctccg cattcccggc tttcagcccg
     4201 tctttgattt catgtcgaac cattggcgcg cggtatacgc cgcgatggtg aaggcactga
     4261 cgacattcag ggagtttttg cggccgcaca acgggatgcg caccacctca tcgcacagct
     4321 tgatcacatc ggcgtccaac ccgaaacgct cattgcccag caccagcgcg cacgggaagg
     4381 cccattccgc ccgttccaga ggtttcgcgt ccgccacggt ctccaacgcc acgcaccgca
     4441 ctccgttggc tttcaatccc gccaacgccg cgcgcacgtc cccgccgtca cgccagcgca
     4501 ccagtttctc ggctccgagt gcggcacgcg ccgtctgcgg ccggtccggc gcgggggtgt
     4561 acccgcagag cacgacctcc gcaaccccga aacactcggc cgtgcggaag atcccgccga
     4621 cattaaaggc cgagcgcaac gaatccgtga ccacggtcag cggcatcagc ggcgcggcgg
     4681 gttcggactc cggagtgagg tcacggtcca gaatctcgat gtcggtgacg cggatgcggg
     4741 caacttcacg ttccaccggc acgagcgcgc accagaactg cgcccgtgtc atgtcgggcg
     4801 taagcagccg ggccagcgca ctcagacggg gttgctcgtg ccgtgcggcc agccacgcgc
     4861 ggcaggcctc cagactgcgg cggcgcaccg gagcgtccgg ccacaccgta tccagcgcca
     4921 gcagtcgctc gcgcgcctca cgccataact gttcgtccgt ttgtttcacc gtaacaggcc
     4981 tccgcgcccc gcccgcaggc ggaaacaaga cagtatgaca caacccggac gtttttcgct
     5041 atggtaaagc cgccccgttt ttggcacact catttgaaaa caagccgaat tatggcagaa
     5101 ctcggaggaa agatgaaaca gcggatgatg atggcggcgg caagcttgac ggcctccgcc
     5161 gtgttggcgg ggttgtatgg ggacacaccg gacgcgcggc acgcgtgggc tgtgcatgat
     5221 cgaaaccggc ccaatccggt caagatcacg gcggagccgg ggatgccgcc ctcggatgcg
     5281 actgtgctgt ttgacggctc ttcgctggaa gcgtgggaga gcaccaaggg cgagcccacg
     5341 aagtggcgcc tggtggacgg agtcttggag tcagtgaaag gcgccggggc cattcgcacc
     5401 aaacagagct tcggtgactg ccaactgcat ctggagtggg ccgcgccgac tcgtgtcgag
     5461 ggcatgggtc agggccgtgg caacagcggg gtctttctca tgagtagcta tgagattcag
     5521 attctggact cgtacgagac gtacacgcta ccggacggca gcaacaagaa tcccaactat
     5581 gccgacgggc aggcggcctc ggtctatgcc gagaacccgc cgctggtgaa cgcctgccgc
     5641 gaaccgggcg cgtggcagac ctatgacatc attttccacc agccgatctg ggagggcgac
     5701 aaactgaagt atcccggttc gctgaccgtg ttgcacaacg gtgtgttggt gcaggaccat
     5761 tgggagatgg agggcctgac cacgcactgc cggcgccggc cgctcgcgcc gcacgcgacg
     5821 aaactgccgt tgcagttgca ggatcacggc aaccccgtgc gcttccgtaa catctggatc
     5881 cgcgagatcc cgtcgcgtta cgccaacacc acccacggcg gcccggccgc aaatgaggcg
     5941 gacgtgatgg ctctgcgcaa agagacggct gccaagctct tcgcccagat tcttccggag
     6001 aacccccgca aggcagaggc cctgcaccag attctggagg tgatctccta cgagaaggct
     6061 gaaccgtaca ccgcactcgc caagaagctg tgcgacgcct atctgaacga actggcgggt
     6121 tgcggggcgc aacaactgga aagccgcaaa ggcgagatca tcggtctgcg caacgcttgc
     6181 aatgtcttga tccgcaacaa gatatacgac gagagatgcc cgttacacgc cacgctccag
     6241 aagatcatca ctgaacaagg gttcgaaaaa aagaagaagt gagaagagaa gcaagaagga
     6301 atacgtaccc tataccccct ttccgtcttg tg
//
LOCUS       DAOSLE010000149         6286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11242_length_6286_cov_5.343444, whole genome shotgun sequence.
ACCESSION   DAOSLE010000149 DAOSLE010000000
VERSION     DAOSLE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_6286_cov_5.343444"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1439
                     /locus_tag="PK576_09055"
     CDS             132..1439
                     /locus_tag="PK576_09055"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE61779.1"
                     /translation="MSGRTMVLGALAIGIASIARATPVIPADSVSVSQNAGRVVTVTY
                     TLGDENNQESAIVTVDFETNVTGTVEGPWVSIGERNFRNVNGFVNKVVEPGTHTIIWA
                     PRETWPDRVIQGGNFRAKVKAWAKTCPPDYLAIDLVAPMRYYYTSTNALPFDIGDDIC
                     RKDILLMRKIPAAGVEWRMGAPDGEKGKGVNNRYTAMETTHYVTLSSDYYFGVFLITR
                     WQYARIMGDSAIASLQKPCQAVTGMSYDLVRYAGQTTAASGNVWPTDKSVNSGSMLYR
                     LRQMSGIDTFDLPTSAQWEFACRAGTSSALYTGLELASSDPNALDSEDTNLNDIAWYR
                     FNTGGVCPDRVGTKLPNGWGLYDLYGTVYEWCLDFARTAWSTNELDPEGPVSGTQRFQ
                     VGGVNNDCPARCRSAFRMGRDPSSAYPGAEIFGVRVVCGVAAP"
     gene            1455..1715
                     /locus_tag="PK576_09060"
     CDS             1455..1715
                     /locus_tag="PK576_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61780.1"
                     /translation="MNTRFLKLSLGLSTIATALSLFADVAWKYDTSARPADDVVEQSA
                     ILGVSSVRPSVAAAVDSGAFCEVASDGCAFDSRPMSMVLILR"
     gene            1733..3037
                     /locus_tag="PK576_09065"
     CDS             1733..3037
                     /locus_tag="PK576_09065"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOE61781.1"
                     /translation="MRYDAGSFALSVLCAATTMAMPVVPTNDVVCVQDPITKIVTVTY
                     VLGDENNQEPAIITLDFETNVTGTAEGPWVSIGAKNYRDLGGAANRLVQAGTNTVTWC
                     PQKGWPNHAVSAAGFRATVRVWTKDDPPDYLVIHLDEPYGQRFYTCEDALPGGIESDI
                     YRRDHLVMRRIHAAGVTWRMGAPIDEVGRSGSLETPRFVTLTSDYYIGVFPFTLWQYC
                     YVQRILPPTCTDEDGACALDTWSWNHARYGGADNQPESGKVWPEDKSLVSGSILYKMR
                     DRFDLLFDYPTAAQWEYACRAGTTTPRFDGSSCAYGWNTRWDSEYEDAAMDAYAWYAH
                     NSTFGHKRVGMKKPNPWGLYDMYGLVYEFCLDWADPSPSNDPITDPIGPDTGTERIFC
                     GSAWNGGVPFLRSAVRSKYLPNVTYAEHRLFGFRVAAPLMDP"
     gene            3171..6083
                     /locus_tag="PK576_09070"
     CDS             3171..6083
                     /locus_tag="PK576_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOE61782.1"
                     /translation="MKAISLGCGIVAACLLGGILYGAETVKAEPKVRGRNHLLPQDFE
                     TPRPDGRFENHMAFAWDYVRRMTPEYSFDKLREASEMPAWRAKVRTKLGELLQIPVPL
                     PKVEFKLLNEEKRNGYRLHRYEFYPEERLAIPILVLVPENAIAGKKRVPAVVCMPGSG
                     ASLNSLAGEPDEFGNRYPARNRQAWHYAQIGMIGVAIENPATAESGIREVNHFATQAQ
                     FARMMTLAGRSNWGFMTKHVLQTIDFMKEHPNVDSERIAVSGMSLGCIPALYAAVMSD
                     DVAAVVYNDFVSSWAANALSVTKNLGGSVDARRPYGFHRWFDDEPDLMAAVAPRPMIF
                     SEGGAWKGVIEKVQRAYRLAGAEQNLRIAYYEKYADPATRKYEDVALHQAKGLTGNDY
                     LLFSNVDAGQHSFHPDVNLPWIAEVFFGKADFTPRQWAMIRASVVWPAQQQMPLDYEV
                     EPPEEKRAAGDTWAGGLGPVVYNTDGCDMLDYPVGDPITYEGFVAQRLARDIPGSGVS
                     AVGYCPVSSGFGFFTALKMGDTLLNPLPPGSRWRGRDVYNATADFAKQGLDALEMGLR
                     YSRSEGRVAVASLRMNDTHDQEHTPEKPYFLFSPFKIRHPECLMGTREKKPPLCAWSA
                     VDYTRPEVRKAMAGYVQQLADNYDFDALDLDFFRHVQYFRSVAWGAESASDEERAMMT
                     DLVRDIRKILDARGAQKGRRLLLSVRTPDSAAYCHAVGLDVERWMKEKLIDVWCGSGY
                     FRLCPWKESAEFAHRYGVKYYASLDESRIERHCARLKLPRLDGRNSRENFAARVAAAT
                     EEGCDGIVFFNIDDYPESERKAIAALVPGKTDGLNKDYFLVNRGSGGYTMEGFLKNGT
                     RFRKLPPVDPGRGDAPQLQANDPYVFEMTVSDDFSFRSAQAVVTAEVLIQPTLPKGQL
                     PTLIVNKTALDAPTVPRKDVITFSIPVSALHRGANRFSLSAPEACQFRDFVLRVRH"
BASE COUNT         1286 a         1701 c         1950 g         1349 t
ORIGIN      
        1 cgtcgaatga acaaaaatac cacgaaaaaa ggattctttt atcgcgaacg ttttagcgtt
       61 gtttttgttc tatacttgtg atgtgttagg ttgtatttgg gcatagttcg ggaaacgcga
      121 aggagattgc gatgagtggt agaacaatgg ttttaggcgc gttggcaata gggattgctt
      181 caatcgcgag ggcgacgcca gtcattcctg cggacagcgt atccgtttcg caaaacgcgg
      241 ggcgtgtggt gaccgtcacc tacacgctcg gtgacgagaa caatcaggag tctgcgattg
      301 tcacggtgga cttcgaaacc aatgtcaccg gaacggtcga aggcccgtgg gtgtcgatcg
      361 gcgagaggaa cttccgtaat gtgaacgggt tcgtgaacaa ggtcgttgaa ccgggcacgc
      421 acacgatcat ctgggccccg cgggagactt ggccggaccg ggtgatccaa gggggtaatt
      481 tccgcgccaa ggtgaaggcg tgggccaaga cctgtccgcc cgactatctg gcgatagacc
      541 tcgtcgcgcc catgcggtac tactacacgt cgacaaacgc attgccgttc gacattggcg
      601 acgacatctg ccgcaaggac attctgctga tgagaaaaat tcctgcggcg ggcgtggagt
      661 ggcgcatggg ggcgcccgat ggtgaaaaag gcaagggcgt caataaccgt tacacggcaa
      721 tggagaccac gcactacgtg acgttatcca gtgattacta ttttggggta ttcctgatca
      781 cccgctggca gtacgcccgt atcatgggtg actcggcaat cgcctccctt cagaaaccat
      841 gccaggccgt gaccggcatg agttacgatc tggtgcgcta cgccggacag acgactgcgg
      901 cgagcggcaa tgtatggccg acggataaat ctgtgaacag cggctcgatg ctctacaggc
      961 tccgccagat gagcggtatc gacacgttcg atctcccgac ctctgcgcag tgggagtttg
     1021 cctgtcgtgc cggaacatcg tcggcgcttt acaccggtct ggaactcgcc tcgtccgacc
     1081 cgaacgctct tgattcggag gacactaacc tcaacgatat cgcctggtac cgcttcaaca
     1141 ctggcggtgt gtgcccagac cgcgtgggaa cgaagctccc gaatggctgg ggactttacg
     1201 acctctacgg gacagtctac gaatggtgcc ttgatttcgc gcgcacagcg tggtcgacaa
     1261 acgagttgga tccggaaggg ccggtgtccg gcacccagcg atttcaggtg ggtggcgtca
     1321 acaacgactg tccggcgcgt tgccgttccg cgtttcgcat gggccgtgac ccaagttcgg
     1381 cttatcctgg tgcggagatc ttcggcgtcc gtgtggtgtg cggcgtggcg gcaccctaaa
     1441 caggaggtaa acgtatgaac acacggtttc taaaattatc tttgggcctg tcgactattg
     1501 cgacggcgct gtcgctgttc gctgatgtgg catggaaata cgacacgtca gcccgtcccg
     1561 ccgacgacgt ggtcgagcag tctgccattc tcggcgtatc gtctgttcgg ccatccgtcg
     1621 ccgctgccgt tgattcgggt gccttctgcg aagtggcgtc cgacggttgc gccttcgatt
     1681 cgcggccgat gagcatggta ttgatcttac gctgaaagaa aggacgttag acatgaggta
     1741 tgatgcaggc tcttttgcgt tgtccgtcct ttgtgcggca acgacgatgg cgatgccggt
     1801 ggttcccaca aacgacgttg tttgcgtgca ggatcccatc acgaagatcg tcaccgtgac
     1861 ttacgtcttg ggtgacgaga acaaccagga acccgcgatc attacgttgg actttgaaac
     1921 caacgtcacc ggaacggctg aaggcccctg ggtgtcgatc ggcgcgaaaa actatcgaga
     1981 tctcggtggc gctgccaacc gcctggtgca ggcgggaacc aacacagtca catggtgtcc
     2041 acagaagggc tggccgaacc acgcggtatc tgccgccgga ttccgcgcca cagtccgggt
     2101 ttggacgaag gatgacccgc cggactatct ggtaatccat cttgacgaac cctacggcca
     2161 gcggttctac acatgcgagg atgccttgcc tggcggcatc gaaagcgaca tctaccgccg
     2221 cgatcatctg gtgatgcggc gtatccatgc ggccggcgtc acttggcgta tgggggcgcc
     2281 gatcgatgag gtcggtcgct ctggaagtct ggagacgccg cggttcgtta cgctcacgag
     2341 cgattattat atcggcgtct tcccgtttac gctgtggcag tactgctatg tccagcggat
     2401 tttgcctccg acctgtaccg acgaggatgg tgcctgtgcg ctcgacactt ggtcgtggaa
     2461 tcacgctcgt tacgggggtg ccgataatca gcctgaaagc gggaaggtct ggccggagga
     2521 caagtctctg gtcagcggtt cgatcctgta caaaatgaga gaccgtttcg atcttctgtt
     2581 cgattatccc actgccgcgc aatgggaata cgcctgccgt gccggcacga ccacgccgcg
     2641 tttcgatggt tcgtcctgtg cttacgggtg gaataccaga tgggattccg aatacgagga
     2701 cgccgcgatg gacgcatacg cttggtatgc ccataacagc acctttggac ataaacgtgt
     2761 ggggatgaag aagcccaacc cttggggact ttacgacatg tacggtcttg tctacgaatt
     2821 ctgtttggac tgggcagatc cttcgccgag caacgatccc atcacggatc cgatcggccc
     2881 cgacactggc acggaacgga ttttttgtgg tagtgcttgg aacggcggcg ttccgttttt
     2941 gcggtcggcg gtgcgatcaa agtacctccc taacgtgact tacgctgaac accgtctctt
     3001 cggcttccgc gtggcggcac cgctgatgga tccctgaccg attattacta gaggctcttg
     3061 caaaaacctt cgtggcatgg gtgttccgcc catgaaccac gggcaagatg cccgtgccac
     3121 acctgtaagt aaggggtttt gcaaaagttc actaaaaaaa ggaatgtaca atgaaagcaa
     3181 tttcgctcgg ctgcggcatc gttgccgcat gtctgcttgg cgggattctg tacggcgcgg
     3241 agacggtaaa agcagagccc aaggtccgag gacgcaatca tctcctgccg caggacttcg
     3301 agacaccccg tccagatgga cggtttgaga atcacatggc cttcgcctgg gactatgtcc
     3361 ggcggatgac gccggagtac tctttcgata agttgaggga ggcctccgag atgcccgcct
     3421 ggcgtgcgaa agtgcggacg aagttgggcg aactcttgca gattccggtg ccgttgccca
     3481 aggttgaatt caagcttttg aacgaggaga aacgaaacgg ctaccggctc catcgctacg
     3541 agttctatcc ggaggagcgt ctcgctatcc cgattctcgt tctggtgccg gagaatgcga
     3601 tcgccgggaa aaaacgggtt ccggccgtcg tctgcatgcc ggggagcggc gcgtcgctca
     3661 attcgctcgc aggggagccg gacgaattcg ggaaccgcta ccctgcgcgt aaccgccaag
     3721 cctggcatta cgcgcagatc ggcatgatcg gcgtggctat cgagaatccg gccacggccg
     3781 agagtggcat acgcgaggtt aaccattttg cgacgcaggc acagttcgcg cggatgatga
     3841 cgctcgccgg acgtagcaac tggggattca tgacgaagca tgtgctccag acgatcgact
     3901 ttatgaaaga gcatccgaac gtcgattccg aacggattgc cgtttcaggg atgtcgctcg
     3961 gttgcatccc cgcgctctac gctgcggtga tgagcgacga cgtagcggca gtcgtctaca
     4021 acgacttcgt ctcctcatgg gcggcgaacg cgctttcggt gacgaaaaat ctgggcggaa
     4081 gcgtcgacgc ccggcggccg tacgggttcc accgttggtt tgacgatgaa ccggatctga
     4141 tggcagccgt ggcgccccgt ccgatgatct tcagcgaagg cggtgcgtgg aagggtgtca
     4201 tcgaaaaggt gcagcgcgcg taccgtctag caggtgcgga gcagaatctg cgcatcgcct
     4261 attacgagaa atacgccgat cctgcgactc gcaaatacga ggacgttgct ctccatcagg
     4321 cgaaggggct tacgggcaac gactatctcc tcttctcgaa tgtggacgct ggacagcaca
     4381 gcttccaccc cgacgtgaac ctgccctgga tcgcggaggt atttttcggc aaggccgatt
     4441 tcacacctcg gcagtgggcc atgatcagag catcggtcgt ctggccggct cagcaacaga
     4501 tgccgcttga ttatgaagtt gagcctcccg aagaaaaacg tgccgcaggt gatacctggg
     4561 caggtggact tggcccggtg gtctacaaca cggacggttg tgacatgctg gactatccgg
     4621 tcggagatcc gatcacgtat gaggggtttg tcgcccaacg gctcgcccgg gacatccccg
     4681 gcagcggggt gtctgcggtc ggctattgtc cggtcagttc cggcttcggg tttttcaccg
     4741 cgctcaagat gggcgatacg cttctcaacc cgctgccgcc gggatcccgt tggcgcggac
     4801 gcgatgtcta caacgctacg gccgatttcg ctaagcaggg gcttgatgcg ctggaaatgg
     4861 gattgcgata ttcgcgttca gagggacgcg tggcggtggc ctcgttgcgg atgaacgaca
     4921 cgcatgacca agagcatacc cccgagaagc cctatttcct tttctcgccg ttcaagatcc
     4981 gccatcctga atgcctgatg ggcacacggg aaaagaaacc gccgctctgt gcatggagtg
     5041 cggtcgacta cacgcggccc gaggttcgaa aagcgatggc cggttatgtg cagcaactcg
     5101 ccgataacta cgactttgac gcactggatc tcgatttctt ccggcacgtg caatatttcc
     5161 ggtctgtcgc ttggggagcg gagtccgcct ccgacgagga gcgggcgatg atgacagatc
     5221 tggtgcggga tatccggaaa atccttgatg cacggggagc ccagaaagga cgtcgattgc
     5281 tcctttcggt gcgcacgccc gattccgccg cctattgtca tgcggtgggg ctggacgtcg
     5341 agcggtggat gaaagagaag ctgatcgacg tctggtgcgg tagcggctat tttcggctct
     5401 gcccgtggaa ggagtccgcg gagttcgccc accgttacgg tgtgaagtac tatgcctcgc
     5461 tggatgagtc acgcattgag agacactgtg cccgcctcaa gctgccgcga ttggacggac
     5521 gcaattcgcg cgagaacttc gcggcacggg tggcggcggc tacagaagag ggctgtgacg
     5581 gtatcgtgtt cttcaacatt gatgactatc ccgaatccga gcgcaaggcc atcgctgcgc
     5641 tggttccggg caaaacggat ggcctcaaca aagactattt cctcgtcaac cgtggttctg
     5701 gcggttacac gatggaaggg ttccttaaga atgggacgcg ttttcgtaaa ctgccgccag
     5761 tcgatcctgg gcgcggggac gcaccgcagc ttcaggcgaa cgatccgtac gtcttcgaaa
     5821 tgacggtttc ggatgatttc tccttccgtt cggcacaggc tgtggtaaca gccgaagtgt
     5881 tgattcaacc tacccttccg aaaggccagt tgccgaccct gatcgtgaat aaaacggcgc
     5941 tggatgcccc gacggttccg cgcaaggatg tcatcacgtt ctcgataccg gtttcagcac
     6001 ttcatcgtgg cgcaaaccgt ttttctctgt ccgcgccaga ggcgtgccag ttccgagatt
     6061 ttgtgttgcg cgtgcggcat tagatggtga taggtcgtgt ttgagcatga gccgggaaac
     6121 gcggcgggat tatctgaaga tcacaaggaa acgaaagtga aaaagaataa gtgtgccatg
     6181 aatctggcag ggatgatggc ggcggttgcc gcatggcagg gtttgggcgc ggaggacgcc
     6241 atcaacatcg gcgggcggcg tgagctgttt gtggatgacg cgatgg
//
LOCUS       DAOSLE010000150         6141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11573_length_6141_cov_8.028590, whole genome shotgun sequence.
ACCESSION   DAOSLE010000150 DAOSLE010000000
VERSION     DAOSLE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11573_length_6141_cov_8.028590"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1395
                     /locus_tag="PK576_09075"
     CDS             184..1395
                     /locus_tag="PK576_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOE61783.1"
                     /translation="MRKILLVCAMATARLVWGQVSDTAKDDAAADRDKALRAQLPGLF
                     RDAAEQYRGMLKQLEGTQDAFPRRWEKGSLVTIGPKEWTSGFFPGSLWLLYEATREET
                     WKREAMRYTAMLESVRHYTGNHDIGFMLYCSFGNGLRLANPDGYREVLLDGAAALCKR
                     YVPSLGMIRSWDSYKNPVIIDNMMNLELLMWAAKQSGEPRFREIAVSHADQTNRRHFR
                     PDGSAYHIVDYNPATGKIFGYYAGQGASADGPWARGQSWGLYGFTLMFRETGTPAYLT
                     RAVSLADFLIGHRNLPADAIPYWDYEAAGIPNEPRDASAAAIMASALLELATFAEAPK
                     AVRYRDTAVRQLLALSSPAYRAPVGENGHFLLMHGVGHLPGNKEIDVPLNYADYYYLE
                     ALLRFRRLQNN"
     gene            1430..1822
                     /locus_tag="PK576_09080"
     CDS             1430..1822
                     /locus_tag="PK576_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HOE61784.1"
                     /translation="MMLSEFVKSADWKNEKHVPVIECADTVQAGKPLAITISVGKETA
                     HPNTTNHHIVWIALHYVPAGAKTSIEIARCEFSAHAAGVEGPDKGPARTCSTVSISAE
                     FAVSGTLYATAYCNIHGLWASEQPITVA"
     gene            1846..5730
                     /locus_tag="PK576_09085"
     CDS             1846..5730
                     /locus_tag="PK576_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61785.1"
                     /translation="MRSRLPLVLGGVGLALLVRAAPLASDLLLWLPCDEGGGAFTEDR
                     SPNRLEAELSGVQWVTGSFGTALRFNGTNALAELPPVPGLNGAERFTLALWAVWEDPA
                     PRRYPCLLSSHTWSPGGMLLFVSDQTCSFRLGRPGHRAGTPGHAWNESSVNLLSDLPQ
                     RRWTHLCVTFDRPHLTSYVGGKPVARTRWDHAVEADALRLGGWSGAVCHNGLIDDLRI
                     YGRALTPSEITALANPSDRADAAWKPATERERAPEPPFARFRTRHAELTLNARGRVTS
                     LRNRASGRELLVRPTPLVSARLRNGQRITARSVTRQGDALTFGFATHAGSAVVAIDAH
                     REFFNFTLRTHSLTDATELVFCELPVRPTRYRGGMANLLSDDEDGVCLRGYDLPVEMR
                     IAGDVLRVSAAVPPGPSGWRAGLAAGPRDTLTTSLRAMARHAGVPMSENSGPWSLEAE
                     ATRGSYLFANLAHAATDDWIEVARRGGFSTLHLHGWWQTLGHYAVRTQYYPRGLDDMR
                     DTVERIHAAGLRAGIHTLTACIDTRDAWITPEASPHLIPFDTYTLARSVSPTDTVLYV
                     NEPPSARHDVVFTYSGNGNAIRIGSEIIQYAAITREPPYAFRDCTRGAFKTRPAAHAA
                     GAQADYLQQRYNAFYPQPDSPLADELADRIAHVFNTCKLDQLYFDGSEGMMSRYGIDA
                     MRHKIMRRLEGNPLIEASCHGAHNWWFHSRLGAWDHPVWAPKRFHDDHVAVCARYRAS
                     DLLAPQMGWWAPRMATPFARGHHLDEMEYFAGKNLGLDAAMSIQGVNVNQAILPYHLE
                     RQFTLLGWYEHLRLARYFDTQTVARLIVPGAEFRLRQTRDGIWQFTPVTMRTHRVTGP
                     DDGSAAWRVTHPHAQQPLCLRVEALYDAAPAATPPAGKSLVGTNDFAAFAVSTASAAV
                     RLNVSEVSEETRGGNRHLRLRAENRGTTSTGAWARAVLTFPAPYRNLSGTGAFGMWVK
                     GDGSGALLNLQVGCPREFMGGLSDHYVTLDFTGWRYVTLLTRERDTARMDEHVWPYRK
                     AGSHALFRTNLDMAHLSQVSLYLNALPAGAHVEVTVSPVEALPVRSVDMKRPVVTVNG
                     QGLTVPFTLASGEFAELEPDGLFTHYSERGEPLARAMCDARGRPVLRAGDNAFTYTGG
                     RAEITVTTFGEAFGASRRRRAIDWKHLKHEYEMPRLVLASNDVTRAEWEVAVRPGEKA
                     RLGVEFSGGMASPAILAQGKTWSFPVTLQAGQRLLCRDGRNWSVLDANRATLSQGTLD
                     TPLPRLNGGPNRVVFTCENPDRARICLVKRYE"
     gene            complement(5750..>6141)
                     /locus_tag="PK576_09090"
     CDS             complement(5750..>6141)
                     /locus_tag="PK576_09090"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614236.1"
                     /note="catalyzes a two-step reaction, first charging an
                     alanine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOE61786.1"
                     /translation="QMVLGPHVQQKGSNITPERLRFDFSHPEKMTPGQIKQVEEIVND
                     VIQRNLPVTMETMTIEEAKAKGATALFTAKYGEQVKVYATGDFSMEVCGGPHAAATGE
                     LGGFKIKKEESSSAGVRRIKAVITAKD"
BASE COUNT         1067 a         2005 c         1955 g         1114 t
ORIGIN      
        1 cattcgaccc gcgacgatcg acagggacgt ccgcgtttcc gacaccgcaa gtcgcccgtc
       61 gcccgtcgcg gggcgacata aggtctgacg ggaaacgcgg gcgtccctac cgaatcgacg
      121 actcgacggg acgcgcgttt tttgttacgg tgatcccgaa tttaaaaccc tggagaatct
      181 cacatgcgga aaatcctttt ggtttgtgcg atggctacgg cgcgcctcgt ttgggggcaa
      241 gtttcagaca ccgccaaaga cgatgcggcg gccgaccggg acaaggcatt gcgcgcgcag
      301 ctcccgggac tgttccggga cgccgccgag cagtaccgcg gcatgctgaa gcaactggaa
      361 ggcacgcaag acgcgtttcc gcgccgctgg gagaagggga gcctcgtgac catcgggccg
      421 aaagagtgga ccagcggctt cttcccgggt tcgctctggc tcctttatga ggccacgcgc
      481 gaagagacgt ggaaacgcga ggcgatgcgt tacaccgcca tgctggagtc ggtccgccac
      541 tataccggca accacgacat cggctttatg ctgtactgca gtttcggtaa cggtctgcgg
      601 ctagccaacc ccgacgggta ccgcgaggtc ctgctggacg gtgccgccgc gctctgcaaa
      661 cgttatgtgc cgagtctggg catgatccgt tcatgggaca gttacaaaaa tccggtgatc
      721 attgacaaca tgatgaatct ggagttgctg atgtgggccg cgaaacaaag cggggagccg
      781 cgtttccgcg agatcgccgt cagccacgcg gatcagacca accggcgcca cttccggccg
      841 gacggcagcg cttaccacat cgtggattac aatcccgcca ccggtaagat cttcggctat
      901 tatgccgggc agggcgcttc ggccgacggc ccgtgggcac gcggccagtc gtggggcctc
      961 tacggattca cgctgatgtt ccgtgaaacc ggcacccccg cctaccttac gcgggccgtc
     1021 agcctggcgg actttttgat cggccaccgt aacctgcccg cggacgccat cccctactgg
     1081 gattacgagg cggcgggtat cccgaacgaa ccgcgcgacg cctcggctgc agccatcatg
     1141 gccagtgcgc tgctcgaact cgcgactttc gccgaagcgc ccaaggccgt ccgctaccgc
     1201 gacaccgctg tgcggcaact gcttgcgctc agttcccccg cctatcgcgc gcccgtcggg
     1261 gagaacggcc acttcctcct gatgcacggc gtcggccacc tacccggcaa caaagagatc
     1321 gacgtaccgc tgaattatgc ggattactac tacctcgaag cattgctgcg tttccggcgt
     1381 ttacagaaca actgacacgc tcacatccca aaccaaccga aaggagtcca tcatgctatc
     1441 cgaattcgtc aagagtgccg actggaagaa cgagaaacac gttccggtca tcgaatgcgc
     1501 cgacacggtc caggccggta agccgctcgc catcacgatc tccgtcggca aagagacggc
     1561 ccatccgaac acgaccaacc accatattgt ctggatcgcc ctgcattacg tgcccgccgg
     1621 ggccaaaacc tcaatcgaga tcgcccgctg cgagttcagt gcgcacgccg ccggagtcga
     1681 ggggcccgac aaaggacctg cccgcacctg cagcacggtc tcgatcagcg cggagttcgc
     1741 cgtctccggc acgctatacg ccacggccta ctgcaatatc cacggcctct gggcctcgga
     1801 gcagccgatc accgtcgcgt gaccgttgcc gtctaaggga aagagatgag atcccgatta
     1861 ccgctcgtgt taggaggggt cggcctcgca ctgttggtgc gggccgcacc gctggcttcg
     1921 gatctgttgc tctggttgcc ttgcgatgag gggggcggcg cttttacgga ggatcgttcg
     1981 cctaaccgtc tggaggcgga actttcgggc gtgcagtggg tcaccggaag cttcggcacg
     2041 gcactgcggt tcaatggaac caacgcgctg gccgaactgc ctcccgtacc cggactcaac
     2101 ggcgcggaac ggttcaccct cgcgctctgg gccgtttggg aggatcccgc gccgcgccgc
     2161 tatccctgtc tgctgagttc gcacacctgg agccccggcg gcatgctgct tttcgtttcg
     2221 gaccagacct gctccttccg gctgggacga ccgggccatc gcgccggaac gccgggccat
     2281 gcctggaacg agagttccgt gaatctgctc tcggacctgc cgcaacgccg ctggacgcac
     2341 ctgtgcgtca cgttcgaccg tccgcacctc acatcgtatg tcggcggcaa acccgtggcg
     2401 cgcacgcgtt gggaccatgc cgtggaagcg gacgcgctgc gtctcggcgg ctggtccggc
     2461 gcggtttgcc ataacgggct gatcgacgat ctgcggatct atggccgggc cctgacaccc
     2521 tccgagatca cggcgctggc caacccctcc gaccgcgccg acgcggcttg gaaaccggct
     2581 acggaacggg aacgtgcgcc cgaaccgccg ttcgcgcgtt tccggacacg gcacgcggaa
     2641 ctgaccctga acgcccgggg ccgggtcacc tcgttgcgca atcgcgcaag cggccgcgag
     2701 ctgctggttc gtcccacacc gctggtttcg gcccgcttgc ggaacggaca gcgaatcacg
     2761 gcgcgttccg taacgcgtca gggtgacgcc ttgactttcg ggttcgcgac ccacgccggt
     2821 tcggcggtcg tcgcgattga tgcgcaccgc gagttcttta acttcaccct gcgtacgcat
     2881 tcgctgaccg acgcgacgga actcgtcttt tgcgaactgc cggtccgtcc cacccgttac
     2941 cggggcggca tggccaacct gctctcggat gatgaagacg gggtctgtct gcgcggctat
     3001 gacctgccgg tggagatgcg gatcgccggg gacgtcctgc gtgtctccgc cgccgtaccg
     3061 ccgggtccct ccggttggcg cgccgggctg gcggccgggc cgcgcgatac cctgacgacc
     3121 agcctgcgcg ccatggcccg ccacgccgga gtgccgatgt cggaaaacag cggcccgtgg
     3181 tcgctggagg cagaggccac acgcggatcc tacctgttcg ccaatctcgc gcatgccgcc
     3241 accgatgact ggatcgaggt cgcgcgacgc ggcggttttt ccaccctgca cctccacggc
     3301 tggtggcaaa cgctgggcca ctatgcggtg cgcacccaat attatccgcg cgggctggac
     3361 gatatgcggg acacggtgga gcggatccat gccgcaggat tgcgcgcggg aatccacacg
     3421 ctcaccgcct gcatcgacac gcgtgacgcg tggatcacgc ccgaggcgag tccgcacctg
     3481 atcccgttcg acacgtacac gctggcccgt agcgtctcgc cgacggatac ggtgctctat
     3541 gtcaacgaac ccccatcggc gcgtcacgat gtggtcttca cttactccgg caacggtaac
     3601 gcgatccgga tcggcagcga gatcatccaa tacgccgcca tcacacgcga accgccctac
     3661 gctttccgtg actgtacccg gggcgcgttc aagacccgcc ccgcagccca tgccgcaggc
     3721 gcacaggctg attatctgca gcagcgttac aacgctttct atccgcaacc tgattcgccg
     3781 ctggcggacg agttggcgga tcgtatcgcg cacgtcttca acacctgcaa actggatcag
     3841 ctctatttcg acggctccga ggggatgatg agccgatacg gtatcgatgc catgcgccac
     3901 aagatcatgc gtcggctgga ggggaatccg ctgatcgagg cgagttgcca cggtgcgcat
     3961 aactggtggt tccattcgcg tctcggggct tgggaccatc cggtctgggc accgaagcgg
     4021 ttccatgacg atcacgttgc ggtgtgcgcg cgttaccgcg cttcggatct gctcgccccg
     4081 cagatgggct ggtgggcacc gcgcatggcg acccccttcg cgcgcgggca ccatctcgac
     4141 gagatggagt atttcgctgg caagaatctg ggactcgacg ccgccatgtc gattcagggg
     4201 gtaaatgtca atcaagcgat cctcccgtat catctggaac gacagttcac gctgctgggc
     4261 tggtacgagc atctgcgtct ggcgcgctat tttgacacgc agaccgtggc gcgcctgatt
     4321 gtgccgggtg cggaattccg gctgcggcag acgcgcgatg gcatatggca gttcacgccg
     4381 gtcacgatgc gcacacaccg ggtcaccgga ccggatgacg gttccgccgc ctggcgggtc
     4441 acccatcccc atgcgcaaca accgctctgt ctgcgcgtgg aggctctgta cgacgccgcg
     4501 ccggcagcaa cccctccggc gggcaagtcg ctggtcggga cgaatgattt tgcggcgttc
     4561 gcggtatcga ccgcctctgc ggccgtgcgc ctgaatgtgt cagaagtatc tgaggaaacg
     4621 cgcggcggca accgccatct gcggctgcgg gcggagaacc gcggcacgac atccaccggc
     4681 gcgtgggctc gcgcggtgct caccttcccc gcgccgtacc gcaatttgag cggcaccggt
     4741 gccttcggca tgtgggtgaa gggtgacggt tcgggtgcgt tgctcaacct ccaggtcggt
     4801 tgcccgcgcg agttcatggg cggcctgtcc gaccattatg tcacgctgga cttcacgggc
     4861 tggcggtatg tcacgctgtt gacgcgcgag cgcgacaccg cgcgtatgga cgaacacgtc
     4921 tggccgtacc ggaaggcggg gtcgcatgcg ctcttccgca cgaacctcga catggcccac
     4981 ctctcacagg tcagcctgta tctcaatgcc ctgccagcgg gcgcacatgt tgaggtcacc
     5041 gtaagtccgg tggaagccct gccggtgcgg tcggtcgaca tgaagcggcc ggtggtgacc
     5101 gtgaacggac aaggtctgac ggtgccgttc acgctggcct ccggcgaatt cgccgagttg
     5161 gaaccggacg gcctcttcac gcactacagc gagcggggcg agccactggc gcgcgcgatg
     5221 tgcgacgcaa ggggacgacc ggttttacgg gccggcgaca atgcgttcac ctacacgggc
     5281 ggacgagcgg aaatcacggt caccacgttc ggcgaggcat tcggcgcatc ccgcaggcga
     5341 cgcgcgatcg actggaaaca tctgaagcat gaatatgaga tgccgcgcct ggtgctcgcc
     5401 tcaaatgacg tgacccgcgc cgagtgggaa gtggctgtgc ggcccggtga aaaagcgaga
     5461 ctgggcgtcg agttctctgg cggaatggcg tcccccgcga tccttgcgca gggcaagacc
     5521 tggagttttc cggtcacgct ccaagcgggg caacgcctgt tgtgccgcga cggacgaaac
     5581 tggtcggtgc tggacgcgaa ccgcgcgacg ctgtcgcaag gtacgctcga tacgccgttg
     5641 ccccggctga acggtggtcc gaaccgcgtg gttttcacat gcgaaaatcc cgaccgcgcg
     5701 cggatctgtc tggtcaaacg gtacgagtga gcgattggat tcgtccgcgt taatctttgg
     5761 cggtgatcac ggccttgatg cggcgcacgc ctgccgagga agactcttcc ttcttgatct
     5821 tgaagccgcc cagctcgccg gtggcggcgg cgtgcggacc gccgcagacc tccatcgaga
     5881 aatcgccggt ggcgtagacc ttgacctgct cgccgtattt ggcggtgaac agggcggtcg
     5941 cgcccttggc cttggcctct tcaatggtca tggtctccat tgtgaccggc agattgcgct
     6001 ggatcacgtc gttgacgatc tcctccacct gcttgatctg cccgggcgtc atcttttcgg
     6061 ggtgtgagaa gtcgaagcgc aggcgctcgg gcgtgatgtt ggagcccttc tgctggacgt
     6121 gcgggccgag caccatctgc a
//
LOCUS       DAOSLE010000151         6128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11602_length_6128_cov_8.113782, whole genome shotgun sequence.
ACCESSION   DAOSLE010000151 DAOSLE010000000
VERSION     DAOSLE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11602_length_6128_cov_8.113782"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /locus_tag="PK576_09095"
     CDS             complement(<1..1336)
                     /locus_tag="PK576_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOE61787.1"
                     /translation="MFLIFILLAVVVAALSGLPGILLRRSDDGGERISSISMVCAACM
                     GLIGAVGGLLGYNGKSLALKWPAVGGGSVGLDALSAFFLVPVFLIGGMGAVYGLSYWP
                     HRRHPVSGLRLRFFWGMLVAGMALLIIARHAMIFLLGWEIMAFSAFFLVTAEGHRIAS
                     RQAGWIYLISTHVSVLALFALFAIWRHVTGSFNMTPAAPQALSQGVTALCMVLVLVGF
                     GLKAGVMPLHFWLPGAHANAPSHVSAIMSGVVIKTGIYGMIRWSALLPDVPVAWGALV
                     LLLGALSGVLGVLFALGQHDFKRLLAYHSVENIGIILMGFGVALLGRAVQRPEWVTLG
                     FGACLLHVWNHGLFKPLLFFCAGAVMRVTGTRQMDQLGGLAKRMPWTAAAFLVGAVAI
                     CGLPPLNGFVSEWLLYLGLLKGAVGTAGGALAALVGVPTLALIGALALACFVK"
     gene            complement(1413..1488)
                     /locus_tag="PK576_09100"
     tRNA            complement(1413..1488)
                     /locus_tag="PK576_09100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Ala,seq:cgc)
     gene            1705..3051
                     /locus_tag="PK576_09105"
     CDS             1705..3051
                     /locus_tag="PK576_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOE61788.1"
                     /translation="MVDGTVLKGYADQIRATGRIDPALYEKFNVKRGLRNADSTGVLV
                     GLTRIGNVHGYVISESEKVSVPGQLFYRGIDVNDLVEGFQSEKRFGFEECAYLLLFGE
                     LPDSRQLEVWQETLDAYRRLPNDFKENAIIRTPGKDIMNSIARAVLMGYIYDSDPDNL
                     DIENALRQSIELIAQFPTIAAYAYQAKAHYHLGQSLMVRYPEKGKSTAENFLMLIRED
                     SLYTQLEAELLDLCLVLHAEHGGGNNSAFTTHVVTSAFTDCYAAVAAATLSLKGTRHG
                     GANLRVMKQMREIKENVENWDREGEVADYLVKILNREAGDGSGLIYGLGHAVYTLSDP
                     RTEMLREKARALAKTKGRDEEMALYETIERIGPEIFNRNRAKPRDLCANVDFYSGFVY
                     DMLNIPIDLYTPIFAISRVVGWCAHRIEELLNGGPIIRPAYKAVFERRPYKPMAER"
     gene            3081..3224
                     /locus_tag="PK576_09110"
     CDS             3081..3224
                     /locus_tag="PK576_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61789.1"
                     /translation="MGILNYNPAMRYRPFGTRTGVLVSALGFGAMRLPQSEGHIDLPA
                     QSI"
     gene            complement(3428..4801)
                     /locus_tag="PK576_09115"
     CDS             complement(3428..4801)
                     /locus_tag="PK576_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOE61790.1"
                     /translation="MLARQDVLSTLRMVREEHLDVRAVTLGINLNVCANPDPERLACG
                     VYERIVAKAGPLVRFCDEVSDKYALPIVNKRIAVSPVSLLLEGHTPETAVRVCQALDA
                     AAEAVQIDLLGGFSALVQKGMTPGEQTLVEALPQALTATRRVCSSFNVGTTRTGIQVD
                     AVNRIARKIIETAQASRATRGFGCAKIVVFANMPEDNPFMAGALLGPGEPECVINVGV
                     SGPGVVKRAVDRLLQNHPAASLDEIAEEIKHTAFRVTRTGELIGREVAERLGIPFGVV
                     DLSLAPTPRIGDSVGEIYQSMGISQIGAPGTTAAVMLLNDAVKKGGAFASSSVGGLSG
                     AFIPVMEDHALSRAVTEGTLTLEKLEAMTAVCSVGLDMILLPGDTSAETVAGIILDEA
                     AIGIANRKTTGVRVIPVPGAACGEFVDFGGLFGSGVVTRPACPTGAAAFVRRGGRIPA
                     PIHSMMN"
     gene            complement(4818..5483)
                     /locus_tag="PK576_09120"
     CDS             complement(4818..5483)
                     /locus_tag="PK576_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61791.1"
                     /translation="MTTSRHPPLRRFVTSVLVPDRVGLLRDITQAVFALEGNIGGIRQ
                     TLVDGFFNLVFTSEHPAAVTPENLRALLDAALESEAVVTVLDRPETPLPVLADGARFV
                     VMTRGEDKPGTILAITSFLVARGINIEDWMVELEAGSVIYLAQVTVPESADFRQLQTA
                     FRQEMAERGLNALLCHENIFRATNEIGPIRALISNRVRDAGPSEQTPPVQNCNPKPET
                     CNV"
     gene            complement(5546..5621)
                     /locus_tag="PK576_09125"
     tRNA            complement(5546..5621)
                     /locus_tag="PK576_09125"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5586..5588),aa:Thr,seq:cgt)
     gene            5894..>6128
                     /locus_tag="PK576_09130"
     CDS             5894..>6128
                     /locus_tag="PK576_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61792.1"
                     /translation="MKPTLIIFFTFLTVCAALAAPDARRELMARVVACDEAADAAVAA
                     IRTPEELAAKQRAWRATWLESLGGLPERTPLEDR"
BASE COUNT         1303 a         1892 c         1872 g         1061 t
ORIGIN      
        1 ccttcacgaa gcacgccagc gccagcgcac cgatcagcgc cagcgtcggc acgccaacca
       61 gagccgccag cgcaccgccc gccgtgccga ccgccccctt caacaggccg agatacagca
      121 gccattcgct gacgaagcca ttgaggggcg gcagtccgca aatcgccacg gcaccaacga
      181 ggaaagcggc ggccgtccac ggcatccgtt tggcgaggcc gcccagttga tccatctggc
      241 gcgtgccggt cacccgcatc accgcgccag cgcagaagaa gagcagcggc ttgaacaagc
      301 cgtgattcca cacatgcagc aggcaggcgc cgaaacccag cgtgacccac tccggacgct
      361 gtacggcacg ccccagcaac gcgacgccga aacccatcag gatgatgccg atgttctcga
      421 cactgtgata ggccaacagg cgtttgaagt cgtgttgccc gagggcgaac aacacgccga
      481 gcacgccgct cagtgcgccc agcagcaaga ccagcgcgcc ccatgcgacc ggcacatccg
      541 gcaacaacgc gctccaccgg atcatcccgt agatcccggt tttgataacg acgcccgaca
      601 tgatcgccga aacatggctg ggggcgttgg cgtgtgcgcc gggcaaccaa aaatgcaacg
      661 gcatcacacc ggccttgaga ccgaacccca ccagaaccag caccatgcaa agggccgtca
      721 ccccttggct caacgcctgc ggtgccgctg gcgtcatgtt gaatgaaccg gtaacatgcc
      781 gccatatcgc gaacagcgca aacagcgcca gcacactcac atgggttgaa atcaaataga
      841 tccagcccgc ctgccgactc gcgatgcgat gcccttcggc ggtgaccaga aagaaggctg
      901 agaacgccat gatctcccag cccaacagga agatcatcgc atgacgcgca atgatcagca
      961 gcgccatgcc cgcaaccagc attccccaaa agaaccgcag acgaagcccg ctcaccggat
     1021 ggcggcgatg cggccagtaa ctgagtccat agacagcacc catgccaccg attaaaaaaa
     1081 ccgggaccag aaagaatgcg ctcaacgcat cgagccccac gcttccgccg ccgacagccg
     1141 gccacttcag cgcaagcgac ttgccgttat accccagcag gccgcccaca gcgccgatca
     1201 agcccatgca cgcggcgcac accatggaga ttgaggatat ccgttcaccg ccatcgtcag
     1261 accgccgcag cagaatgccg ggcaggccgc tcaaggctgc aacgacgaca gccagcaaaa
     1321 taaagatgag aaacaccagc gctcctttca tccgcgaccg cgtcaggtct caccacacaa
     1381 aaaaagaccg ccgagcggcg gccctttcat catggtggag cgaaggagat tcgaactcct
     1441 gacccccacg ttgcgaacgt gatgctctac caactgagct accgccccat gaatcccaaa
     1501 aagtgcagta catgatgcca aagagggggc cgttcggcaa gcgtgatttt tcgcgattca
     1561 tccgcgagaa agagttgacc cctcctaggg aacccgctac actcacggct ccttaaaagc
     1621 gggtagtgtt tgttccgata ggtaaacacg ggtatttttc cgcaaaagga ggcagggatg
     1681 aagacagagc aatccgaaca tctgatggtg gatgggacgg ttctgaaggg ctatgcggat
     1741 cagatccgcg ccacgggacg aatcgatccg gcactatacg agaagttcaa tgtcaagcgc
     1801 ggattgcgta acgcggatag tacgggtgtg ctggtgggac tgacccgcat cgggaatgtg
     1861 cacggctatg tgatctccga gagcgagaag gtctccgtgc cgggccaact cttttaccgg
     1921 ggcatcgacg tcaatgatct cgtggaaggc ttccagagcg agaagcgttt cgggtttgag
     1981 gagtgtgcct atctgctgct tttcggcgag ttgccggact cgcggcagtt ggaagtgtgg
     2041 caggagacgc tggacgcgta ccgtcggttg ccgaatgact tcaaagagaa cgcgatcatt
     2101 cgcacaccgg ggaaagacat catgaacagc atcgcgcgtg cggtgctgat gggctatatc
     2161 tacgattcgg atcccgacaa tctggacatc gagaatgcgc tgcggcagag tattgaactc
     2221 atcgcccagt tcccgaccat cgccgcgtac gcttatcagg ccaaggctca ctaccacctg
     2281 gggcagagcc tgatggtgcg gtatccggag aagggcaaga gtacggccga gaatttcctg
     2341 atgctgattc gcgaggacag cctctacacg cagttggagg cggagctgct ggacctgtgt
     2401 ctggtgctgc acgcggagca cggcggcggc aacaactcgg ccttcaccac gcatgtggtg
     2461 acctcggcgt tcaccgactg ctatgcggcg gtcgctgcgg ccacgctctc gctcaaaggg
     2521 acgcgtcacg gcggtgccaa ccttcgcgtg atgaaacaga tgcgcgagat caaggagaat
     2581 gtggagaact gggaccggga gggcgaggtt gccgattacc tcgtcaagat cctgaatcgg
     2641 gaggcgggtg acggtagtgg actgatctac gggttgggcc acgcggttta cacgctctcc
     2701 gatccgcgga ccgaaatgct gcgcgagaag gcgcgcgcgc tggccaagac caaagggcgc
     2761 gatgaggaga tggcgctgta cgagaccatc gagcgcatcg gtcccgagat cttcaaccgt
     2821 aaccgcgcca aaccgcgtga tctatgtgcc aacgtggatt tctactcggg attcgtctac
     2881 gacatgctca acatcccgat tgatctctac acgccgatct tcgccatttc gcgcgtggtc
     2941 ggctggtgcg cccaccggat tgaggagctg ctcaacggcg ggcccatcat ccggccggcg
     3001 tacaaggcgg tttttgagcg gcggccctac aaaccgatgg cggagcgctg acgggcgcct
     3061 gccgcgtccc aacggcggac atggggattc tcaattacaa tccggcgatg cgataccggc
     3121 ccttcggaac ccggaccggg gtgctggttt ccgcgctcgg gttcggcgcg atgcgcctgc
     3181 cgcaaagcga agggcacatt gacctgcccg cgcaatcgat ttgatcgaaa ccggcttgga
     3241 cggaggcatc aactacatcg acaccgccta tgcctaatct acaacaggca cctgctggtc
     3301 gaccgcaaga acacgttgca accagccctc taccgtagtt ttctgccgaa cgaaaaggct
     3361 tcgggctgca tccggtgcgg cacctgcgca aagcgctgtc cgcagtcgat cgacatcccc
     3421 gtccgtttca gttcatcatg ctgtggatcg gcgccgggat tcgtccaccg cgccgcacga
     3481 aggccgcggc acccgtgggg caggccgggc gcgtcaccac gccggacccg aacaggccgc
     3541 cgaaatccac aaactcgccg catgccgcgc cgggtaccgg gatgacgcgc accccggtgg
     3601 ttttgcggtt ggcgattccg atagcggcct cgtcgagaat gatccccgca acggtttcgg
     3661 cggaagtgtc gccgggcagc aggatcatgt ccagccccac ggaacagacc gcagtcatgg
     3721 cctcgagttt ctccagcgtc agggtgccct cggtgacggc ccgggagagg gcgtgatcct
     3781 ccatcaccgg aatgaaggcg ccggaaagtc cgccgaccga gctggaagcg aaggccccgc
     3841 cctttttgac ggcgtcgttg agcagcatca ccgccgcagt ggtacccggc gcgccgatct
     3901 gactgattcc catgctttgg tagatctcgc ccaccgaatc accgatccgc ggtgtgggtg
     3961 cgagcgaaag atccaccacg ccgaacggga taccgaggcg ttcggccact tcgcggccga
     4021 tcagttcgcc ggtgcgcgtc acgcggaagg cggtgtgttt gatctcctcg gcgatctcat
     4081 caagcgatgc cgccggatga ttctgcaaca gccggtccac ggcgcgtttg accacgccgg
     4141 gtccactgac accgacattg atgacgcact ccggttcacc ggggccgagc agcgcgccgg
     4201 ccatgaaggg gttgtcctca ggcatgttgg cgaagaccac gatcttggcg cagccgaagc
     4261 cgcgcgttgc gcggctggct tgtgcggtct cgatgatttt gcgcgcgatc cggttcacgg
     4321 catccacctg aatacctgtc cgggtggtgc ccacgttaaa tgaggagcag acacgacggg
     4381 ttgcggtcag agcctgcggc agggcttcga ccagggtctg ttcgccgggg gtcatcccct
     4441 tttgcaccag ggctgaaaag ccgccgagca aatcgatctg caccgcttcg gctgccgcat
     4501 cgagcgcctg gcagacccgt acggcagttt ccggcgtgtg gccttcgagc agcaggctca
     4561 ccggcgagac cgcgatgcgt ttgttgacga ttggcagcgc gtacttgtcg cttacctcat
     4621 cgcagaaccg aaccagcgga ccggccttcg cgacgattcg ttcatacacc ccgcaggcga
     4681 ggcgttccgg atcggggttg gcgcatacgt tgaggttgat gccgagggtc acggcccgca
     4741 catcgagatg ttcctcgcgc accatgcgga gggtggagag cacgtcttgt ctggctagca
     4801 tagggatatc cggcaggtta cacgttacag gtttcaggtt tcggattgca gttctgcacg
     4861 gggggtgttt gttctgacgg cccggcatcg cgcaccctgt tgctgatcag cgcccggatc
     4921 gggccgatct cgttggtggc gcggaagatg ttttcgtggc agagcagcgc gttcaggccg
     4981 cgctcggcca tctcctgccg gaacgcggtt tgaagctggc ggaagtctgc ggactcgggc
     5041 accgtcactt gggcgaggta gatcacgctg ccggcctcca gttccaccat ccagtcttca
     5101 atgttgatcc cgcgcgcgac cagaaacgag gtgatggcca gaatcgttcc cggtttgtcc
     5161 tctccacgcg tcatcaccac aaagcgggca ccatccgcga gaaccggtaa cggggtttcc
     5221 gggcggtcga gcacggtcac aaccgcttcg gactccagcg cggcgtccaa cagggcgcgc
     5281 aggttctcag gtgtcacagc ggcgggatgt tcggaggtga agaccagatt gaaaaagccg
     5341 tcgaccaatg tctggcggat ccctccgatg ttgccttcga gcgcgaacac ggcctgggta
     5401 atgtcccgca acaacccgac gcggtcgggc acgagcaccg atgtgacgaa ccgccgcagc
     5461 ggcggatggc gcgatgtggt catgataacc ctgtgaaaaa ggcgaaaccc cgaaaatcgc
     5521 ggataccggg tttcggggtt tcaaatggag ccaacgatcc ggatcgaacg gacgacctga
     5581 tcattacgaa tgatctgctc taccagctga gctacgttgg cttgaaaaac ggatgtacta
     5641 taccgattcg tccgcagaga agtcaagcgc gctgtccgcg cagccaaaaa tccgcagtgt
     5701 aatctggaaa tccttgcaaa acgctgcgcc agcatgtaaa aaagagccgt aatcatcatc
     5761 gggtcgtaac gcgcagacat ggaaaaactg caatgaaaac aatttcagtc cattgtgtca
     5821 gcaccgccgt gttttatttg gcggaattct gcgtggcgcg gagacggagt ccgaatgtgc
     5881 cagtgggatg ctaatgaaac ctactctaat catcttcttt acctttttaa ccgtctgcgc
     5941 ggctcttgcc gcgccggacg cacggcgcga actaatggcg cgcgttgtcg cctgtgacga
     6001 ggcggcggac gccgctgtag cggcgattcg cacgccggag gaactggctg cgaagcagcg
     6061 ggcgtggcgt gcgacgtggc tggagagcct cggcggcctg ccggagcgaa cgccgctcga
     6121 agaccgca
//
LOCUS       DAOSLE010000152         6102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11665_length_6102_cov_7.674880, whole genome shotgun sequence.
ACCESSION   DAOSLE010000152 DAOSLE010000000
VERSION     DAOSLE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11665_length_6102_cov_7.674880"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..440
                     /locus_tag="PK576_09135"
     tRNA            367..440
                     /locus_tag="PK576_09135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Gln,seq:ctg)
     gene            complement(442..6033)
                     /locus_tag="PK576_09140"
     CDS             complement(442..6033)
                     /locus_tag="PK576_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE61793.1"
                     /translation="MQRKKGLIACGLVGSALFLGVFILRGFHSRRTTPDAPPRTDAAP
                     AAALPRPAMHRLPQLTTSPAATSATHTTTATTTVGVAACLTEAEIEPLRRAFKPPRFD
                     ADPSLPRERQTLLSNRLLISPVERVLPRAASGAQSPTPRDTTPFLVLFNTPVTEASRA
                     LLIEAGAIPRGFFPNNALLAELTPAILAALAAMPSVQAAEEFVPGDKIQPFLASLLAS
                     HPAETRVRMTVQTLAPEDAESVAAAVTAAGGEVEGFTANTRWGLVQAILPLGAVRTLA
                     TRGEVQWIEERPQPKLFNDFAARGTHLNTTNVWIDWGLTGRGQIAGHADTGLDTAVEE
                     TLHPDFRGRVVALFARGRPGDASDLDGHGTHTAGSLCGDGTASGGQYRGMAWEAGLAH
                     QSVANASGALTGLPYDLYELFEETYACGARVHSDSWGSDTYGTYTSDSRSTDLFAWDH
                     PDHLAVFAAGNAGRDSDRNGVVDPGSVGSPATAKNALAVGATENDRLPGTGGYSAYTW
                     GAAWPNRYPVEPIAGDYLSYSATLNPRYLQGMAAFSSRGPTLDGRVKPDVVAPGTDVL
                     STQSAVGDPVWRAFGANPRYCFGGGTSMSAPLIAGTALLLRQYATERAGITNPSAALI
                     RAMMAGGARSLTPGQYGTTLFREIPAISPNSVEGWGQPDIRDTVHPRRRMVRLYDGIH
                     PGAGQTNLFLIPVSVSHTPLDLVLAWTDYPATAGAGVTRVNDLDLLVTAPNGTVLYPN
                     GGTERDGVNTIERLHLPVAMPGTYIVRVIGTDVPFPGGSAALYVRGGFDDPPLIVHAP
                     VGDRIADGTDLTVRFRVQAVSPFASGEVFMNWASGDATGPTGVWQRAIAEFEGMDGSG
                     FLARIPAQPAGGYVHYTLRVVTKKHDVRLPAAEGSSFSFYVGRPVTLRVSGVPEAYGS
                     VTPPYGTNTVISEIAFVASAPESVTLGKGRRMACSGWTGEGDVPAVGASNRVSLSLSG
                     PSALVWQWRGEVALTNRYRLADVNAIFGETVRWVPEGASAETETALELGFVGNVPYAF
                     CGWRVDGTRWPDADSTSPNPATGIAMYAPRLAEGDYLPFWLDSDDNVLSDWWELRYFG
                     SATGGRDNQDDPDNDGWTNLGEFLDNTDPRDAASQPTPPAITVFALAPLQRDRPPWGV
                     RAEITDNLTVEETLLFWRESGDAAWRQSDMSWIEGNLYGAEIDPPAHGLKTVEYYVAA
                     ADLVGWYDSSFRSTSAVYSVTGAYGEPWMTVTPHAFGLFELSGDATNLAVTVANLAGP
                     DLLWTASVATASAPFAARDEDWVPGGMAGAWCLTTNRTWDGDEVWYCGNPATRRYADS
                     CHAWLDTPVFTVGQGGGALFRQWLRTEWDSGAYFWDGAVVRVSTNLGASFEIITPEGG
                     YPGRIMENPASPFAAHQPCLAGTGGWETVTLDLAAFAGRQVIVRLEFGSDAYVTDEGW
                     YVAGFRVFSLAESAPAWLDPQGTWGGTVPAGSEESFGVRLDSAPLAPGDEVAACLRVT
                     GNDPVGGRVLPVTVRRGHRLSVTAEGPGSVTTDRFFLFRRLGATVILQAHPNAYLDTL
                     TRTGFPVEHFDYRTRVQTVFLGQVETDQTVSGHFTQGLWTLTVVDPYRVGSPANGTYS
                     LTNNTLVEASALTPLLLSDSIRRTCEGWFLSGHTPGTGTTANLTFAITNHATLIWRWM
                     LHYRVQTGVEGGGGRIEPAEAWVPQYERIELTALPDTGYRFDNWLAVPGNATVTSNRI
                     HFTVLAPQFVRASFLAETTRTGVPHLWLAAFGLGNDWEAAALEDVDGDGMPAWAEWLA
                     DTDPTDARSYLALTGLALENDLGRIRWIGGHARTQVLEFATSPAGPWRPVATNLPPTP
                     ITNTVMLPITGDAGFYRLRIP"
BASE COUNT         1128 a         2022 c         1962 g          990 t
ORIGIN      
        1 aatttatagg caaggagttt ttcacttcga cgttctgagt tcggcgttgg gcgttgggcg
       61 ttcaaaggcg gcgtgaaacg ccgcccggac gagcagacgc tcgtccctcc cctccttacg
      121 gtcaaagaca aaaagaaaaa tttgcgcaat ctgtgcaagt ctgtggataa aaccagaaaa
      181 cgcaagacac gttgttgcgg gcggggacgc ccgcgctccc gactctctgc tccccttttc
      241 accgggaacg catgatcttc gcacgcccct gtcacaaacc gattacggca aagcgaaaaa
      301 caggattgcc tttatgccat gcctttggct atcttattta ctcctttttg caatgaaaag
      361 gcgttttgcc tcatggtgta acggtagcac agcagactct ggatctgttt gtttaggttc
      421 gaatcctaat gaggcaacca cttacggaat gcggagacgg tagaaacccg cgtctccggt
      481 tatgggcagc atcacggtgt tggtgatcgg ggtaggggga aggttggtgg cgaccggacg
      541 ccaggggcca gccggactcg tcgcgaactc gagcacttgg gtacgcgcgt gaccgccgat
      601 ccaacggatc cggcccaagt cgttttcgag cgcgagtccc gtaagcgcca gatatgagcg
      661 ggcgtcggtc ggatcggtgt ccgccagcca ctcggcccaa gcgggcatgc cgtcgccgtc
      721 cacatcctcc aaggcggcgg cctcccaatc gttgcccaaa ccaaacgcgg ccagccacag
      781 atggggtaca cccgtgcgag tcgtctcggc aaggaaggag gctcggacga actgtggcgc
      841 caacaccgtg aaatggatgc ggttgcttgt gaccgtagca ttgcccggca cggccagcca
      901 gttatcgaaa cgatatcccg tgtccggaag ggcggtcagt tcgatccgtt cgtactgcgg
      961 gacccaggcc tccgccggtt cgatccgccc gccgccgccc tcgactccgg tctggacgcg
     1021 gtaatgcagc atccaccgcc agatcagcgt ggcgtgattg gtgatggcaa aggtgaggtt
     1081 cgccgttgta ccggtgccgg gcgtatgtcc tgagaggaac caaccctcgc aagtccggcg
     1141 aatcgaatcg gaaagcaaca gcggcgtgag ggccgaggcc tcgacgagcg tgttgttggt
     1201 caggctgtag gtcccgttcg ccggagaacc gacacggtag gggtcgacca ccgtcagggt
     1261 ccataggccc tgcgtgaaat ggccgctgac cgtctggtcc gtttcaacct gccccagaaa
     1321 cacggtctgg acgcgcgtcc ggtagtcgaa atgttccacc gggaagccgg tccgtgtcag
     1381 ggtgtcgagg taggcattgg ggtgcgcctg caatatgacg gttgcgccca gcctgcggaa
     1441 gaggaaaaac cgatcggtcg taacgctgcc cggcccctcg gctgtcaccg aaagccggtg
     1501 gccgcgccgc acagtgacgg ggagcacccg gccaccgacc ggatcgtttc cggttacgcg
     1561 caaacatgcc gccacttcgt ctcccggcgc gagcggcgca gaatcgagcc gcacgccgaa
     1621 gctttcttcg ctgcccgccg gaaccgtacc cccccaagtg ccctgcggat cgagccatgc
     1681 gggcgcggat tcggcaaggg agaagacccg gaacccggcc acataccacc cctcgtctgt
     1741 gacgtaggcg tcggaaccga actcgaggcg cacgatgact tggcgtcccg cgaaggcggc
     1801 cagatccaag gtcaccgtct cccagccacc cgtacccgcc agacaaggct ggtgcgccgc
     1861 aaagggggaa gcagggttct ccataatccg gccgggatac ccgccctccg gcgtgatgat
     1921 ctcaaagctc gcccccagat tcgtggagac gcggaccacg gcgccgtccc aaaaataggc
     1981 accgctatcc cattcggtcc ggagccactg gcggaagagc gcaccgccgc cctgaccaac
     2041 cgtgaaaacg ggcgtgtcaa gccaggcatg gcaactgtcg gcatatcgac gcgttgcggg
     2101 attgccgcaa taccacactt cgtcgccgtc ccacgtccgg ttggtggtga gacaccacgc
     2161 tccggccatg ccgcccggaa cccagtcttc gtcccgcgcg gcgaacggcg cggaagctgt
     2221 agccacgctg gcggtccaga gcagatccgg tcccgcgaga ttggctaccg tgaccgcgag
     2281 attcgtggca tcgccgctca attcgaacag accgaaggca tgcggcgtga ccgtcatcca
     2341 gggctccccg taggcgccgg tgaccgaata caccgcgctg gtggaacgga acgaggaatc
     2401 gtaccagcca accagatccg cggccgccac ataatattca accgtcttca gcccgtgcgc
     2461 gggcgggtcg atctccgccc cgtacagatt cccttcgatc caggacatgt cgctctgccg
     2521 ccatgccgcg tccccggact cgcgccagaa gagcagggtc tcctccacgg tcagattgtc
     2581 ggtgatctcg gcccgcacgc cccatggcgg acggtcgcgc tgcagcggcg cgagtgcgaa
     2641 aaccgtgatc gcaggcggag tcggctggct cgccgcatcg cgcgggtccg tgttgtcgag
     2701 aaactcgccg aggttggtcc acccgtcgtt gtccggatca tcctggttat cccggccgcc
     2761 tgtcgcactg ccgaaatagc ggagctccca ccagtcgctc aacacgttgt cgtcactatc
     2821 caaccagaaa ggcaggtaat ccccctcggc cagccgcggg gcatacatcg cgataccggt
     2881 cgcagggttg ggcgacgtcg aatccgcgtc cggccagcgc gtgccatcta cgcgccaccc
     2941 gcagaaagcg tagggcacgt tacccacgaa gccgagttcc agcgcggtct cggtctcggc
     3001 cgacgcgcct tctggaaccc agcggacggt ctcgccgaat atcgcgttca cgtcggcgag
     3061 tcggtagcgg ttggtgagcg cgacctcgcc gcgccactgc cagactaagg cgctcggtcc
     3121 gctcaggctc agactcacgc ggttggaggc gcccaccgcc ggcacatcgc cctcgccggt
     3181 ccagccggaa caggccatac gccgcccctt gccgagcgtc acgctttcgg gtgcggaggc
     3241 cacaaacgcg atctcggaga tgacggtgtt ggtaccgtag ggcggcgtca cactgccgta
     3301 cgcctccggt acgcccgaca cccgcagggt caccggtcgg ccaacataga aggagaagga
     3361 tgaaccctcc gcagcaggga gccgcacatc gtgttttttg gtgaccaccc gcagcgtgta
     3421 atgcacgtaa cccccagcgg gctgtgcggg gatccgcgcc agaaatccac tcccgtccat
     3481 gccctcgaac tccgcgattg cccgctgcca gacaccggtc gggcccgtag cgtctccgga
     3541 tgcccagttc atgaagacct cgccggacgc gaagggagac acggcctgca cccggaaccg
     3601 caccgtgaga tccgtgccgt ccgcgatacg gtctccgacc ggggcatgga cgatcagcgg
     3661 cggatcgtcg aaccccccgc gcacgtaaag cgctgcgctg ccgccgggga aaggcacgtc
     3721 ggtcccgatc acccgcacaa tataggttcc cggcatcgcg accgggaggt gcagccgttc
     3781 gatcgtgttg accccgtccc gctccgtgcc accgttagga taaagcacgg ttccgttggg
     3841 cgcggtcacc agcagatcga gatcattgac gcgcgtcacg cccgcgcccg ccgtggccgg
     3901 ataatcggtc cacgccagca ccagatccag cggcgtgtgg ctcacgctga ccgggatgag
     3961 aaaaaggttg gtctgccccg caccggggtg gatgccgtcg tagagccgca ccatgcgacg
     4021 ccgcgggtga accgtatccc tgatatccgg ctggccccat ccctccaccg aattgggcga
     4081 aatcgccggg atttcgcgga agagcgtggt gccgtactgg cccggcgtga gcgaacgcgc
     4141 gccgccggcc atcatggcgc ggatcagcgc ggccgagggg ttggtgatcc cggcgcgttc
     4201 cgtggcgtac tgccgcagca acagagccgt gcccgcaatc agcggggcgg acatgctggt
     4261 gccgccgccg aaacagtaac gcgggtttgc gccgaaggcg cgccagaccg gatcgccgac
     4321 cgcggattgg gtggagagca catccgtacc gggcgcaacc acgtcgggtt tgacacggcc
     4381 atcgagcgtg ggtccgcggc tggagaaggc ggccataccc tgcagataac gcggattcag
     4441 cgtggcgctg taggagaggt aatctccggc gatcggctcg accgggtagc ggtttggcca
     4501 ggccgcgccc caagtgtagg ccgaataccc gccggtgccc ggcaagcggt cgttctccgt
     4561 ggcacccacg gccagcgcgt tcttggccgt ggcaggcgag cccaccgaac ccgggtccac
     4621 cacaccgttg cgatcactgt cacgaccggc gttacccgcc gcgaagaccg cgagatggtc
     4681 agggtgatcc cacgcaaaga ggtcggtaga gcgcgagtcg gaggtgtagg tgccgtaggt
     4741 gtccgaaccc caactgtcgg agtgcacgcg tgcaccgcag gcataggttt cctcgaaaag
     4801 ttcgtacagg tcgtacggca gacctgtgag cgcacccgaa gcgttggcca ccgactgatg
     4861 cgcgagcccc gcctcccacg ccatgccacg gtattgtccg ccgctagccg taccgtcccc
     4921 gcacagcgag cctgcggtgt gtgtgccgtg tccgtccaag tccgaggcat cacccggtcg
     4981 gccgcgggcg aaaagcgcca ccacgcggcc gcggaaatcc ggatgcagcg tctcctccac
     5041 agccgtgtcg agtccggtgt ccgcgtgccc cgcgatctgg ccccggccag tcagacccca
     5101 gtcgatccaa acgttcgtgg tgttgaggtg cgtaccacgc gcggcgaagt cgttgaacaa
     5161 cttgggttgc ggccgctcct cgatccactg cacctcgccc cgggtcgcca gcgtccgcac
     5221 cgcgccaagc ggcaggatcg cctgcaccag gccccagcgg gtgttggcgg tgaacccctc
     5281 gacctcaccg cccgccgcag tcacggcagc cgccacgctc tcggcatcct caggcgccaa
     5341 ggtctgcacc gtcatccgta cccgggtctc tgctggatgc gaagcgagga gcgaggcgag
     5401 aaagggttgg attttgtcac cgggcacgaa ctcctcggcc gcctgcacgc tgggcatggc
     5461 cgcaagcgcg gccagtatag caggggtcag ctcagccaac agcgcgttgt tcgggaaaaa
     5521 gccgcgcggg atcgcccctg cctcgatcaa gagcgcgcgt gaggcttcgg ttaccggcgt
     5581 gttgaaaagc accagaaagg gcgtggtgtc gcgcggggtg ggagactgcg cacccgaggc
     5641 ggcccggggc aggacccgtt caaccgggct gatcagcaga cggttggaga gcagcgtctg
     5701 gcgttcacgc ggcagggacg ggtccgcatc gaaacgcggc ggcttgaagg cccggcgcag
     5761 cggttcgatc tccgcttccg tcagacacgc cgccacgccg acggtagtgg tggcggtggt
     5821 ggtgtgcgtc gctgaggtcg ccgccgggga tgtcgtcaac tgcggcagcc tgtgcatcgc
     5881 tggacgcggc aatgccgcgg caggcgcggc atccgtccgg ggcggcgcgt caggcgtcgt
     5941 acggcggctg tggaaccccc gcaggataaa aacccccaga aacagcgcgc tcccaaccaa
     6001 gccgcacgca atcaaaccct ttttgcgttg catgaccgga agcataacac aagacggaaa
     6061 gggggtatag ggtacgtatt ccttcttgct tctcttctca ct
//
LOCUS       DAOSLE010000153         6080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11722_length_6080_cov_6.876017, whole genome shotgun sequence.
ACCESSION   DAOSLE010000153 DAOSLE010000000
VERSION     DAOSLE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11722_length_6080_cov_6.876017"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            477..1295
                     /gene="panB"
                     /locus_tag="PK576_09145"
     CDS             477..1295
                     /gene="panB"
                     /locus_tag="PK576_09145"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOE61794.1"
                     /translation="MKTWNAASVTAAKGVTRLACVTAYDYASARLADAAGLPLLLVGD
                     SLGMTVLGYESTLPVTLEQMLHHTAAVARAARNAMVVADMPFLSYQVCEAEAIRNAGR
                     FLQEAGADAVKLEGGAFRAGLVSALTQNGIPVLGHIGLTPQSVNQLGGYKVQGKSREA
                     GERLIADARALAEAGAFAIVLECTPPDIAATITAAVPVPIIGVGAGPVCDGQMLVMHD
                     LLGLSEGRPPRFVKAYAALAETMTQAFRTYAEEVRDGVYPAHEHCYAPRKPGST"
     gene            1315..2733
                     /locus_tag="PK576_09150"
     CDS             1315..2733
                     /locus_tag="PK576_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HOE61795.1"
                     /translation="MPPSRTSREDIVITGMACRFAESPSLAAFWRNLLQRQSLLTPLP
                     VPSPTEPAPPRRTLFDRPYPTLCGQLGDLYACTPADQYFPRKMNAGENQDVFFTVQLA
                     IDALRDSGSTLNSLPTDRVSLHLGYAPPFNAASVNWLQHTFFIDQTLGIVQNFFPGVG
                     AEQIEEVRKQLTASLPEPNPYAFLSALGCVIASWTAHYLGFAGPAFLVDAGAVSGHQT
                     LQGAMDDLITRRSDIALAGAVQPPFSDAALQGFAGAIPFSRGKTLQPFSREADGTLPG
                     EGGAMFVLKRLKDALRQNDRIYAIIRSIGIAAAAMDQHHRVPTPERLTRAISRALHAA
                     DITPESVQLIEAHGSAIPHSDQTELKVLHDLYGDRRRGQALTGVGSVKGNIGHTLWSA
                     GAAGILKAALAIAHRTLVPTVSVDKPHLSLASAKSPLYLLSDARPWLRGNRKNPRRAC
                     VSAIDFTGTCAAAILEEYPEDP"
     gene            2730..6026
                     /locus_tag="PK576_09155"
     CDS             2730..6026
                     /locus_tag="PK576_09155"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOE61796.1"
                     /translation="MSSAPHLYDAFDSELILIGASNDDDLALTVRRVLGFLEHAPDVP
                     LQDVAYTCAVSARHMPSILAVVASAVPDLRDRLSLALRKLEARANRIRDKSGTYFFRE
                     RLRPSGRIAFLFPGAVSFYPDMLRDLCLAFDACRGAFDELQEALQTQDPECITPADYL
                     FPPSACYRNDTGVFQTDGFSESFIAAHAANTAFYRLIDSMGVTPDGLTGFSGGDFAAL
                     DVAGVYGNLSRNKRIVFLREGYQMLNRLAEREDLPTCIMLSVLDASRERIDELIAAHP
                     GRMALTCFHSPRQLTVALSPEILKEVTASLQASGAKTMPVPMNRPFNTPWCSKVLPPI
                     EQFLSHWVRHTPRIPVYSCATAERLPAQPRSILKLTADQWTSPILFDDTIRQMYQDGF
                     RLFIELGARGNMTTAVDETLKNLPHQAVAINRIHRSGLVQLHHALGQLAAQGVQLDLT
                     VLHRHRRQHRLDFDRPLALAVTTEHTMRLSSQLPRLTVFAPRGDLLTAAAPPAGTTRK
                     SRAPRETGRHLDFGADFPMLTNAEILEEQAGILELAKVLTLEDYPFLRDYAIGTSQLS
                     YANPELKGLTLLSMISGLEMMTETARKLVPRRRVAQIDNLRATRWVGFERGAVRIILR
                     AERIAWPDPQFSAVRVQLRDGAPNSAFTWPIIEATVLLTAAPAVMPPIHPPPLTRARP
                     VNWSGHDIYPDRLFHGDCLRIVRHVDLWSEEGIDFEIEVPERSQAVRVTRIPLFSIWP
                     MLLDGIVSSFSLWRSHERFAGAISLPFRARRILFYAPGFTEGARLRGYLRLVSVTPRS
                     HVADIQISDGNGNLLIHFRGWEELCERVPPEYQQFILHPSEKFLTQPLPLELLGNPTT
                     PVAGAVATDIPFSLFEHNQELWLKTLAFVLLSPTEREEWLEMQGATSRRVEWLFGRAA
                     AKESARRFLRQFHQARWTAADIPIWPDDSGKPHLLGPWREHTAVTLDLSIAHTSKLIV
                     AAVAANARLGIDIEAIGRDLSEDFTKGVFTNEELERAAQTGEAPTAILRFWCAKEALS
                     KALGTGIRYSPRDLLIRAIDPVTGQIEIELLGQWLEPFKQLKGRRNLVSTSLYEGHVF
                     ASCLLPASLF"
BASE COUNT         1047 a         2151 c         1714 g         1168 t
ORIGIN      
        1 cagggacgcc cgcgtttccg acaccgcagg tcgcttttca aggtttcaag gtttctggtt
       61 tctgggtttc atccaaatcg gggtcggggt cgctatcggg atcgggatcg atcatttaac
      121 cacgaacaca cgggatggca ctgctcgggt ttttatccgc agatttacgc agattacgca
      181 gattttttgg gcatcgctcg ggtttttctg aacgctaggg ggaattcctg attttcatga
      241 agtttcaggg tttcatccaa atcgaggtcg ggatcgctat cgggatcgac gtcccgttcg
      301 cgtcctcgtt ttcgtgcagg tgtacgggca ccgccacgct gtgaacatgt acgacgagcg
      361 agcggaaaga acgcgcaccg ttcacgttat cggtcaggaa aacaggatca cgcccgtttg
      421 gtcagaaccc taacacggat attgccctgt ccgcctgtta tggtaaaata cgtctcatga
      481 aaacatggaa tgcggcttcc gtcactgctg ccaagggggt gacccggctg gcctgtgtca
      541 cggcctacga ctacgccagc gcgcgccttg ccgatgccgc cggccttccc ctgttgctgg
      601 tcggcgactc gctcggcatg acggttctcg gttacgagtc caccctgccc gtcacgctgg
      661 aacagatgct ccaccacacc gctgccgtgg cgcgggccgc gcgcaacgcg atggtggtgg
      721 cggacatgcc tttcctctct tatcaggtct gcgaggccga ggcgatccgc aatgccggcc
      781 gttttctgca ggaggctggt gcagatgccg tcaaactcga aggcggcgcg ttccgtgccg
      841 ggctggttag cgcgctgacg cagaacggga tccccgtgct cgggcacatc ggcctcacgc
      901 cgcagtcggt caaccaactc ggcggctaca aagttcaggg caaatcgcgt gaggcgggcg
      961 aacggttgat cgcggacgcc cgggcgttgg cggaggcggg cgccttcgcc atcgtgctcg
     1021 agtgcacccc gcccgatatc gccgccacca tcaccgcagc cgttcccgtc ccgatcatcg
     1081 gggtcggggc ggggccggtc tgtgacggcc agatgctggt aatgcacgat ctgctcggcc
     1141 tttccgaagg ccggccgccc cgtttcgtca aggcctatgc cgctttggcg gagaccatga
     1201 cgcaggcttt ccgcacctac gccgaggaag tccgtgacgg agtttatccg gcacacgaac
     1261 actgttacgc gccccggaaa cccggatcga cctaacccgc cccacccttt ttctatgccc
     1321 ccttcccgca catcacgcga agatatcgtc atcaccggca tggcgtgccg gttcgccgaa
     1381 tcgccgtcac ttgccgcgtt ctggcgcaac ctgctccagc gccagtcgtt gctgaccccg
     1441 cttcccgtcc catcccccac cgaacccgcg ccgccccgtc gcactctgtt cgaccgcccc
     1501 taccccacac tctgcggcca gttgggtgac ctgtacgcct gtacgcccgc agaccagtat
     1561 ttcccccgta aaatgaacgc gggcgaaaac caggacgtgt ttttcaccgt tcaactcgcg
     1621 atcgacgccc tgcgcgacag cggcagcacc ctcaacagct tgccaacgga ccgggtctcg
     1681 ctgcacctcg ggtacgcccc gcccttcaat gccgcctccg tcaactggct gcagcacacc
     1741 ttcttcatcg accagactct cgggatcgtg cagaatttct tcccgggcgt cggcgcggag
     1801 cagattgagg aagtacgcaa acagctaacc gcctcgctgc ccgaacccaa cccgtacgcc
     1861 tttctctcgg ccctcggttg tgtcatcgcc tcctggaccg cccattatct cggattcgcc
     1921 gggcctgcct ttctggtgga tgcaggcgct gtttcgggtc accagaccct gcagggcgcc
     1981 atggatgacc tgatcacgcg ccgttcggac atcgcgcttg cgggtgcggt gcagccgccg
     2041 ttcagcgacg ccgccctgca ggggttcgcg ggcgcgatcc cgttctcccg cggcaaaaca
     2101 ctccagccgt tcagccgcga agccgacggc accctgcccg gcgagggcgg cgcgatgttt
     2161 gtcctcaaac gcctcaagga cgccctgcgc cagaatgacc gcatctacgc catcatccgc
     2221 tccatcggca tcgcggctgc cgccatggat cagcatcatc gcgtccccac gccggaacgg
     2281 cttacacgcg ccatcagccg tgccctgcat gcggcggaca tcactccgga atcggttcaa
     2341 ctgatcgaag cccacggatc cgcaatcccg cactcggacc agaccgaact caaggtgttg
     2401 catgacctct acggtgatcg gcggcgcggc caggccctga ccggtgtggg gtccgtcaaa
     2461 ggcaatatcg gtcacaccct gtggtccgcg ggcgcggcgg gcatcctcaa ggccgccttg
     2521 gccatcgcgc accgcactct ggtgccgacc gtatccgtag acaaacccca cctttccctc
     2581 gcttccgcca aatcccccct ctatctgctc tccgatgcgc gtccgtggct gcggggcaac
     2641 cgcaagaacc cgcgtcgtgc ctgcgtctcg gcgatcgact tcaccggcac atgcgcggcc
     2701 gcaatcctcg aagaataccc ggaggaccca tgagcagtgc ccctcatctg tatgacgctt
     2761 tcgacagcga actgatcctg atcggtgcgt ccaacgacga tgatctcgcg ctgaccgtcc
     2821 ggcgtgtgct cggttttctg gagcacgcgc cggacgtgcc gctgcaggat gtcgcctaca
     2881 cttgcgccgt ttccgcccgc cacatgccct ccattcttgc ggtggtcgcc tccgccgtgc
     2941 ccgacctgcg cgaccgcctg tcgctggccc tgcggaaact ggaggctcgc gccaatcgga
     3001 tccgcgacaa gagcggcacc tatttcttcc gtgagcgcct gcgccccagc ggccggatcg
     3061 ccttcctctt tcccggcgcg gtctcgttct atccggatat gctgcgcgat ctgtgcctcg
     3121 ccttcgacgc ctgccgaggc gccttcgacg agttgcagga agccctgcaa acccaggacc
     3181 ccgaatgcat caccccggcc gattacctgt tcccgccctc ggcctgttac cggaacgaca
     3241 ccggcgtctt ccaaaccgac ggattctcgg agtcctttat cgccgcgcac gccgcgaata
     3301 ccgcttttta ccgtctcatc gacagcatgg gggtcacgcc cgacggattg acagggttca
     3361 gcggcggcga tttcgcggct ctggatgtgg ccggcgtcta cggcaacctc tcccgcaaca
     3421 agcgtatcgt cttccttcgg gaaggctatc agatgttgaa ccggctggcg gagcgcgaag
     3481 acctgcccac ctgcatcatg ctctccgtgc tggacgcctc gcgcgaacgg attgacgaac
     3541 tgatcgcagc acatcccggc cgaatggcac tgacctgttt ccacagtccg cgccagttga
     3601 ccgtggccct ctcgcccgaa atcctcaaag aggtcaccgc ttccctgcaa gcatctggcg
     3661 cgaagaccat gcccgttccg atgaaccggc cgttcaacac gccgtggtgt tcgaaagtcc
     3721 tcccgcccat cgagcagttc ctttcgcatt gggtgcggca cacgccgcgc atcccggttt
     3781 attcatgcgc caccgctgag cgtctgcccg cccaaccgcg gagcatcctg aaactcaccg
     3841 ccgatcaatg gacctccccg attctgtttg acgacacgat ccggcagatg tatcaggacg
     3901 gcttccgcct gttcatcgaa ctcggcgcgc gcggaaacat gaccaccgct gtcgacgaaa
     3961 cactgaaaaa cctgccccat caggctgtcg ccatcaaccg catccaccgt tcggggctgg
     4021 tccagcttca ccatgccctc gggcaactcg ccgcccaagg ggttcagctc gacctcaccg
     4081 tgcttcaccg ccaccgtcgg cagcaccggc tggatttcga taggccgctc gccctcgccg
     4141 tcaccacaga gcacacgatg cgcctctcct cgcaattgcc gcgcctgacc gttttcgccc
     4201 cacgcggcga cctcctcacc gcagccgccc ctccggccgg caccacgcgt aaatcccgcg
     4261 ccccccgcga aaccggacgc catctcgatt tcggcgccga cttcccgatg ctgaccaatg
     4321 cggagattct cgaagaacag gcgggcatcc tcgaattagc gaaggtccta accctcgaag
     4381 actatccgtt cctgagggac tatgcgatcg gcacctcgca actctcctac gcgaatccgg
     4441 aactgaaggg gctcaccctg ttgtccatga tctcgggcct ggaaatgatg accgagaccg
     4501 cgcgcaaact cgtgccgcgt cggcgcgtcg cacagatcga caatttacgc gcgacgcgct
     4561 gggtcggctt tgagcgcggt gccgtccgga tcattctccg cgccgagcgg atcgcctggc
     4621 ccgacccgca gttctccgcg gtccgtgtcc agctccgcga tggcgcgccc aacagcgcct
     4681 tcacctggcc gatcattgag gccaccgtgc tgttgactgc cgcacctgcg gtcatgccgc
     4741 ccatccaccc cccgcccctc acccgggcgc gtccggtgaa ctggtcgggg cacgacatct
     4801 atcccgaccg tcttttccac ggcgactgtt tgcgcattgt ccgccacgtc gacctctgga
     4861 gcgaggaggg cattgatttc gagatcgaag tgccggaacg cagtcaggcg gtgcgtgtca
     4921 cccgcatccc gctcttttcg atctggccca tgttgctgga cgggatcgtc tcgagtttct
     4981 cactctggcg ttcgcatgaa aggttcgccg gcgcgatctc gctgccgttc cgcgctcgcc
     5041 gcatcctctt ctatgccccc ggcttcacgg agggcgcgcg cctgcgcggg tatctgcggc
     5101 tggtctcggt caccccgcgc tcgcatgtgg cggacatcca gatctccgac ggcaacggca
     5161 acctgctcat ccacttccgc ggctgggagg agttgtgcga gcgggtcccg cccgagtacc
     5221 agcagttcat cctgcacccc tcggaaaagt tcctcacgca accgctgccg ttggaactgc
     5281 tgggcaatcc gaccacaccg gtcgccggtg cggtcgcgac cgatatcccc ttcagcctgt
     5341 tcgaacacaa tcaggagctc tggctcaaaa ccctggcctt tgtgctgttg tcccccacgg
     5401 aacgtgaaga gtggctggaa atgcaaggcg ccaccagcag gcgggtcgaa tggctcttcg
     5461 ggcgcgccgc cgcaaaagag tcggcgcgcc gcttcctgcg gcaatttcat caggcgcgct
     5521 ggaccgcggc ggacatcccg atttggcccg acgactccgg caagccgcac ctgctcggcc
     5581 cctggcgcga gcatacggcc gtaaccctcg acctctccat cgcccatacc tctaagctta
     5641 ttgttgcggc cgtagctgcc aacgcgcggc taggtatcga cattgaagcc atcggacggg
     5701 acctgagcga ggatttcacc aaaggcgtgt tcaccaacga ggagttggaa cgggccgcac
     5761 agaccggcga ggccccaact gccatcctgc gcttttggtg cgcgaaagag gccctctcga
     5821 aagcgctcgg aaccggcatc cgctacagcc cgcgggatct gctgatccgc gccatcgacc
     5881 ccgtcaccgg ccagatcgaa atcgaactcc tcggacaatg gctggaaccg ttcaaacagt
     5941 tgaaaggccg caggaatctc gtcagcacat cgctctacga ggggcatgtc ttcgcctcct
     6001 gcctgctgcc ggcctcgctg ttctgagaac cgtaaaccta taatcctaaa aagagcacag
     6061 gtttctggtt tcagggtttc
//
LOCUS       DAOSLE010000154         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11899_length_6014_cov_7.413828, whole genome shotgun sequence.
ACCESSION   DAOSLE010000154 DAOSLE010000000
VERSION     DAOSLE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11899_length_6014_cov_7.413828"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..880)
                     /locus_tag="PK576_09160"
     CDS             complement(50..880)
                     /locus_tag="PK576_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE61797.1"
                     /translation="MGVGKREKGVFAVGAGVLVLAAGIAAFVQYRRVPGASSGGQTVA
                     VPDAAETAAAGSGARATTGKPVRSGSVRGGGSSLANAAQRTREREAKRLASPSPTNEV
                     PLTPEQKLAATMEERLDNGDERGALELARQLLKAESSEVRSDVVTTLGWIGVKALPEL
                     SVMLGDADSEVSEEALQQWIMAIEEIEDPHAKAQMLALAMRSTRDEDTLQSIAMEFNG
                     LPEDVVLRSLQTIIQGQKNGPAVDVAREEYESITGEEYSTPEALEKYILENVEPEDEE
                     "
     gene            complement(890..1993)
                     /locus_tag="PK576_09165"
     CDS             complement(890..1993)
                     /locus_tag="PK576_09165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE61798.1"
                     /translation="MRRTVSRHHGVRRRGAVWSGLLLSGMLLAGCGRNGGRAGIVVGD
                     TEKLISEMTPDEVIVSVNGTPFRRADYDLMLSGLDTLYRANNPSANPMSLEMNRKRRA
                     RTLVREFITKEVLVQEARRRGVKATEAHWKDASRSLAKLAASKNVPVEWFAGAPEAEL
                     RLAYQIARENALILALREAEFGDRLKITEADVEWARQSMAQYNESCDQTNRIVLARGE
                     AICKRIRSGEDFFEVAREVTEDTEVPDGVLGEQVPIEIDDKRVRKAAFSLPVGAVSDP
                     FQTEEGLVIIKVLERKGIDSPLAREQATVNLGRILLLTAHPVAEKSDAELRREIMQTR
                     LKKLQAPWLKALQERTRFEYPNGTNLFRKGKAG"
     gene            complement(1993..4698)
                     /locus_tag="PK576_09170"
     CDS             complement(1993..4698)
                     /locus_tag="PK576_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOE61799.1"
                     /translation="MKMKLVAGVCAGVACATLFAGAARAAIPTAGAADPVVPGQWHSN
                     LEAATDKAKELNAPMFVVWGTEGCAYCRSFDTILQNETVREYFANSGFILVYEHSLAA
                     AQTPIRRWVGTGQWPLTRLTWWQDGVKVADQKWQRPSTSKDPATVLQVLQNFFEQHVG
                     AFSPVPADTSKDAYDPENDAAEGAAELAWGDQEKTEANLKLASKGGDEPYADSADWYR
                     LPVVSGATYRVWFSDVSGIEGDAPQAAVYGDAGGGSVWAGPAVLASEPEFEFVPTAAG
                     TAYIKVWRETSADTNILYTLHHQRFAPGTIEFVQNAVAVNERAGSVTLQVRRTGGTTG
                     EATVQIGYKDKLTPDSGHTATAGQDFNGAPSPATLTWADGEAAVKTVTFTLIKNVIEW
                     EGDETFGVTLASTTGNEVGPEAVVTLNEADPMVTRAADYTGLVEADADDEDPDERVTG
                     TFEMKVSARGQISGKAIFPRNAPGYAGTFAIRNARHHNINIDTGIATIVGSLVQGRTA
                     VPFELELHMESGLIEGVIGTDEDARSCRMFRADLSARAELLASLQGSYTVAFPVADIV
                     WPLEGAPAGSGYATITVDRRGTFRAAGKLPDGTAYSQSGALFVWPGDGTLGVNGLPRV
                     YAVLFTAPSAYQGGRFSGVLCFGDANANGVWDVTAVEDYPFECVSFKPTSVPVYNLEA
                     PGFDLMLGVAGGRYDRYASLHTVFDGFRFFVDEMKKPGTLLRYTVSTVALNEMTGRSF
                     TARIAKEAECVSWESADPLEVAPNASGRGFIVPRGDLVKTGTDGETGKPLYNYDTGTN
                     PNQFQLRLTSSSGLLNGSFRVHYDYATYINLTREPAVRRWAHKSNMIRYNGVLLQEQA
                     VWGAEMTVGYGHYLYSSLAEYPGTARTYRFTQSEEIRLLAIQEGD"
     gene            complement(4815..5525)
                     /locus_tag="PK576_09175"
     CDS             complement(4815..5525)
                     /locus_tag="PK576_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61800.1"
                     /translation="MKKLMMFAAAMTIVGGAFAQCGDPEIPEVNCALVYDVKASVKTT
                     VGDSKAGYTVASNCGDSTNIAAATCFRKIGKKTLQGFLFTCDCECDAIESADMYLWNK
                     KEKVFESGSMSWDFINIIGKKQKDVEGSWKITLDGTGSLGAGTIYGAGFGKWDSKKEI
                     VKSLSGNFAGYLAAPECDDELSCDAAAAYVCTDLLTPDSSLDTAAFGTWSIKYSSSKS
                     KKLAADASAYIDWLPDYL"
     gene            complement(5758..>6014)
                     /locus_tag="PK576_09180"
     CDS             complement(5758..>6014)
                     /locus_tag="PK576_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOE61801.1"
                     /translation="RIPNGSLGRCLITHYQIPVQIGDVTVKPGDVIFADCDGVVCVPR
                     DIACDVLLRAEEIRENEKKIFGWVANGETVKQITDKGGYF"
BASE COUNT         1095 a         2094 c         1618 g         1207 t
ORIGIN      
        1 cctgaaaccc tgaaaccctg aaacctgaaa ccctgaaacc caagcagggt tactcctcat
       61 cctcaggctc gacattctcc aggatatatt tttcgagcgc ttcgggcgtg ctgtactctt
      121 caccggtgat gctttcgtat tcctcgcgcg ccacatcgac cgcagggccg ttcttctggc
      181 cctgaatgat ggtttgcaga cttcgcagga ccacgtcctc gggaagtccg ttgaactcca
      241 tcgcgatcga ttgcagcgta tcttcatcac gcgtggacct catggcgaga gccagcatct
      301 gagccttggc gtggggatcc tcgatctctt caatggccat gatccactgc tgcaacgcct
      361 cctcagagac ttcgctgtcc gcatcgccaa gcatgacgga gagttcgggt aacgccttga
      421 ctccgatcca ccccaaggtc gtgaccacat ccgaacgcac ttcgctgctt tcggccttca
      481 gcaattgacg cgccaattcc aacgcgcccc gctcgtcccc gttatccaga cgctcttcca
      541 tcgtggcggc aagcttctgt tcgggcgtca gcggaacctc gtttgtgggg gagggcgacg
      601 ccaagcgttt ggcctccctt tcgcgagtcc gctgggccgc gtttgccagc gacgatcccc
      661 ctccccgaac gctcccgctc cggacaggtt ttcccgttgt cgcccgcgca ccgctgcccg
      721 ctgccgccgt ttcagccgca tccggcacgg caaccgtttg tccgccggaa gaggccccgg
      781 gaacccggcg gtattggacg aacgccgcga ttcccgccgc caacaccagc acccccgccc
      841 cgacagcgaa gacccccttt tcccgtttgc ctacacccat gaagcccccc taccccgctt
      901 tgcccttgcg gaataaattc gtgccgttcg gatactcgaa ccgcgtccgt tcctgcaacg
      961 ccttcagcca aggggcttgc aatttcttaa gacgcgtctg catgatttcc cgcctcagtt
     1021 ccgcgtcgct cttttccgcg acgggatgcg ccgtcagcag cagaatccgt cccaaattga
     1081 ccgtggcctg ctccctcgca agcggagaat cgatcccctt gcgttccaga accttgatga
     1141 tcaccaaacc ctcttccgtc tggaacggat cggagaccgc gccgaccggc agcgagaacg
     1201 ccgccttccg aaccctcttg tcatcgattt cgatggggac ctgttccccc aaaaccccgt
     1261 cggggacctc cgtgtcctcg gtcacctcgc gcgcgacctc aaaaaagtcc tcccccgaac
     1321 ggatccgttt gcagatcgcc tcgccgcgcg ccagaacaat ccggttcgtc tggtcgcacg
     1381 actcattgta ttgcgccatc gactgcctcg cccactcaac atccgcttcg gtgatcttca
     1441 gccggtcccc gaactccgct tcgcgaagcg ccagaatcag cgcgttctcg cgcgcaatct
     1501 gataagcgag acgcagttcg gcttccggtg ccccggcgaa ccattcgacc ggcacattct
     1561 tgcttgcggc gagcttggcc aagcttctgc tggcatcttt ccaatgggct tcggtcgcct
     1621 tcaccccgcg tcggcgcgcc tcctgaacca aaacctcttt ggtgatgaac tcccgcacca
     1681 gagtcctggc gcgccgtttc cggttcatct ccaaggacat cggattggcg gaggggttgt
     1741 tggcgcggta cagcgtgtcc agcccggaca acatcaggtc ataatccgcg cgccggaacg
     1801 gggtgccgtt cacggacacg atgacctcat ccggcgtcat ctcggagatg agtttttccg
     1861 tatccccgac cacgattccg gcacggcctc cgtttcgtcc gcatccggcc agcagcattc
     1921 cggacagaag gagtcccgac cacaccgctc cccgcctcct caccccatgg tgccgcgaga
     1981 cggttctcct catcaatcac cctcctggat cgccagcaag cggatttcct ccgactgcgt
     2041 aaacctgtag gtccgcgccg ttccgggata ttccgccagg ctcgaataca gataatgtcc
     2101 gtatcccacg gtcatttccg cgccccaaac agcctgctct tgcagcagaa ccccgttgta
     2161 ccggatcatg ttgctcttat gagcccaccg cctgaccgcc ggttcccgcg tgagattgat
     2221 gtaggtcgcg taatcgtaat gcacgcggaa cgaaccgttc agcagtccgc tcgatgatgt
     2281 caggcgcaac tggaactggt tcggattggt ccccgtgtcg taattgtaga gcggttttcc
     2341 ggtctctccg tcggttccgg tcttgaccag gtccccgcgc gggacgatga accctctccc
     2401 gctcgcattc ggcgccactt caagcggatc ggcggactcc cacgacacac actccgcctc
     2461 tttggcgatt cgcgccgtga atgaacgtcc ggtcatctcg ttaagcgcca ccgtgctcac
     2521 tgtgtagcgc aacagggtgc ccggcttttt catctcgtcg acgaaaaagc ggaacccgtc
     2581 gaaaaccgtg tgcagcgagg cgtaccggtc atatcttcct ccggccaccc cgagcatcag
     2641 atcgaaaccg ggggcttcca gattgtacac cggaacggac gtcggcttga aactcacgca
     2701 ttcgaacggg taatcctcca cggccgtgac gtcccaaacg ccgtttgcgt tcgcgtcgcc
     2761 gaagcagagc acacccgaga agcgtccgcc ctgatacgca gagggcgcgg tgaacagcac
     2821 cgcataaacc cgcggcagtc cgttcacgcc caacgtcccg tcccccggcc aaacgaacag
     2881 tgcgccgctc tgggaatacg ccgtgccgtc cgggagtttg ccggcggcgc ggaaggttcc
     2941 gcgcctgtcc acggtgatcg tcgcgtagcc cgatccggcg ggcgcgccct ccagcggcca
     3001 cacgatatcc gccaccggga aggccacggt atacgagcct tgcaggctgg cgagcagctc
     3061 ggcgcgcgcg ctgagatcgg cccggaacat ccggcacgaa cgcgcgtcct catccgtgcc
     3121 gatcacccct tcgataagcc cgctctccat gtgcagttcc agttcgaacg gaaccgcggt
     3181 ccggccctgc accagcgagc cgacgatcgt cgcgatgccg gtgtcgatat tgatgttgtg
     3241 gtgccgcgcg ttgcggatcg cgaacgtccc cgcgtaaccc ggcgcgttcc gcgggaaaat
     3301 cgccttgccg gagatctggc ccctggcgga caccttcatc tcgaacgtgc ctgtgacgcg
     3361 ctcgtccgga tcctcgtcat ccgcatccgc ctccaccagt ccggtatagt ctgccgcgcg
     3421 cgtcaccatg gggtcggcct cgttaagcgt caccaccgct tccggcccga cctcgttccc
     3481 cgtcgttgat gccagggtca cgccgaacgt ttcatcaccc tcccactcga tcacgttctt
     3541 gatcagcgta aacgtcacgg tcttcacggc agcctcgccg tccgcccacg tcagcgtggc
     3601 cggcgaaggc gcgccgttga aatcctgccc cgccgtggcc gtatgcccgg aatcgggggt
     3661 cagcttgtcc ttatacccga tctgcaccgt cgcctcgccg gttgtgccgc ccgtgcgccg
     3721 gacctggagc gtcacgctgc ccgctctctc attcaccgcc acagcgttct gcacgaattc
     3781 gatcgtgccc ggcgcgaagc gctggtgatg gagcgtgtaa aggatattgg tgtccgcgga
     3841 agtctcgcgc cagaccttga tatacgcggt tcccgcggct gtcggcacaa actcgaattc
     3901 cggctcactc gccagcacgg cgggcccggc ccacaccgac cctccgccgg catcgccata
     3961 caccgcggcc tgcggcgcat ccccctcgat cccgctcaca tccgagaacc atacccggta
     4021 ggtcgcgccg ctcaccaccg gaagccgata ccagtccgcg gaatccgcat agggctcatc
     4081 gccgcctttg ctcgcgagct tgaggttggc ctcggtcttt tcctggtcgc cccaggccag
     4141 ttccgccgcg ccctcagccg cgtcgttctc cgggtcatag gcgtctttgg aggtgtccgc
     4201 cggcaccggc gagaaggccc cgacatgctg ctcgaaaaag ttctgcagca cctgcagcac
     4261 cgtggccgga tccttactgg tgctggggcg ctgccacttc tgatctgcga ccttcacgcc
     4321 gtcctgccac cacgtcaggc gcgtcagcgg ccattgccct gttccgaccc accgcctgat
     4381 gggggtttgg gcagcggcca aggaatgttc gtacaccaga ataaacccgc tgttcgcgaa
     4441 atactccctc acggtctcat tctgtaagat cgtgtcaaaa ctgcggcaat acgcgcagcc
     4501 ctctgtgccc cacaccacaa acatcggcgc gttcagttcc ttggccttgt ctgtcgcggc
     4561 ctcaagattg ctgtgccact gccccggcac caccggatcg gcagcccctg cggtcgggat
     4621 cgctgcccgc gccgcccccg cgaaaagagt cgcgcacgcc actccggcac atacaccagc
     4681 aactaacttc atcttcattt gtggtctcct aatgatccgt ccatggatag cgtaccgaat
     4741 ccatgggccg catgcaaata aaaaaagagc gggggtttcc ccccgccctt cactgaaacc
     4801 gtcagctctc tgacttacag atagtcaggc agccaatcga tgtacgcgga agcgtcggca
     4861 gccagcttct tcgacttcga agagctgtac ttgatcgacc aggttccgaa cgcagccgta
     4921 tccagcgagg agtcaggcgt cagcagatcg gtgcagacat acgccgcagc cgcatcgcag
     4981 gagagctcgt cgtcgcactc gggcgccgcg aggtaacccg cgaagttgcc gctcagcgac
     5041 ttcacgatct ctttcttgga atcccacttg ccgaagccgg cgccatagat cgtgccggcg
     5101 cccagcgagc ccgtgccgtc caaagtgatc ttccaggaac cctcaacatc cttctgcttc
     5161 ttgccgatga tgttgatgaa atcccaagac atggaaccgg actcgaacac cttctccttc
     5221 ttgttccaga ggtacatgtc ggcgctctcg atggcatcgc actcgcaatc gcaagtgaag
     5281 aggaagccct ggagggtctt cttgccgatc ttgcggaaac aggtggccgc ggcgatgttc
     5341 gtggaatcgc cgcagttcga ggcgaccgtg tagcccgcct tcgagtcacc gaccgttgtc
     5401 ttgacgctcg ccttcacgtc atagaccagg gcgcagttga cttccggaat ctccgggtca
     5461 ccgcactgcg cgaatgcgcc gccaacgatc gtcatcgcgg cagcaaacat catcaacttc
     5521 ttcatgagta ctcctttgtt gtttctcttt ctgaaaccgc cttgcgactc ctaaacatcg
     5581 ctggcgactc caaaaatcgt gtgcaggtta gcataataag aatatgccgt caacccgcta
     5641 aaatcaaaaa aaactctggt ctttcaggca cccgcccgaa accttctgtg cttcgaatca
     5701 gtgtcaatgt aacacagcgg catggtgcgc gcaagcggga aaacgaaaaa aaagccctta
     5761 gaaatatccg cccttatcgg tgatctgttt gaccgtctcg ccgttggcga cccagccgaa
     5821 gatcttcttc tcgttctccc ggatctcttc ggcgcgcagg agcacgtcgc aggcgatgtc
     5881 gcgcggcacg cagaccacgc cgtcgcagtc cgcgaaaatc acgtcgccgg gcttgacggt
     5941 cacgtcgccg atctggacgg ggatctggta gtgcgtgatc aggcagcggc cgagcgagcc
     6001 gttggggatg cggt
//
LOCUS       DAOSLE010000155         6006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11921_length_6006_cov_7.756512, whole genome shotgun sequence.
ACCESSION   DAOSLE010000155 DAOSLE010000000
VERSION     DAOSLE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11921_length_6006_cov_7.756512"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="PK576_09185"
     CDS             <1..1245
                     /locus_tag="PK576_09185"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61802.1"
                     /translation="DTGSMFLGLCIATFPLMTGSRKELAASLGMPLLVMGVPLFDTLL
                     AIWRRSVRACLPSGLVEVGKRVRVMQPDQDHVHHRLLRQTMNQKTAAIILYVISGVLV
                     LIGLVGTMLKGRAPGLFLIAFVVAVYIVVRHLERVELWDTGRLLSGRHGMMRQGLLIP
                     LYIVWDVFALCGTWMVVRWESGLSLNRAAVLSELPVFIVPVFMALVATKTYARVWGRA
                     QIRDFVVLGLSVLAGTAIGAGLIWLLNDYGCGMIRFAVRFGTLAIFPIAGVRAWRECV
                     RGVMQMLERRVLLENPSALRFLAYGGGLRFRAYLRELMERPGNHDRVIIGILDDEVNL
                     KGRIIAGYPVLGDFETVRSYAADNSIKGVIITCLLPEAKLRWVTAELEAMGLVVSVWA
                     CGERVLANPKGLVVREIGKGET"
     gene            1242..1658
                     /locus_tag="PK576_09190"
     CDS             1242..1658
                     /locus_tag="PK576_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOE61803.1"
                     /translation="MKSYRKELWFKTDHRREYRNITPEVEACLAESGIQEGFCLVNAM
                     HITASVFVNDDEAGLHADFERWLERIAPEKPHSQYAHNGAEDNADAHLKRQVMGREVV
                     VAVTRGKLDFGPWEQIFYGEFDGLRSKRVLVKIIGE"
     gene            complement(1667..2710)
                     /gene="pdxA"
                     /locus_tag="PK576_09195"
     CDS             complement(1667..2710)
                     /gene="pdxA"
                     /locus_tag="PK576_09195"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOE61804.1"
                     /translation="MADARIRCALTLGDGAGVGPELALRALADEELSRTCQLLVYGNR
                     TLLERVAVASGIPFAATIKILDGAEGPLPQDGHVLFDFPFAEADSITPGTVQAACGRQ
                     AYEWVRRATLDISRNQVAHALVTAPLNKSALHAAGVQAVGHTEILSELTETPDPRMLF
                     YAPAFCVCLATIHEPLARVSELLTVSSLLRTLRLTHEACRMFGTPAPRLGVLALNPHA
                     GENGLLGTEERRVLMPAIETARAEGIHASGPLVADTAFTWLLSTPPQSAPYDAYVAMY
                     HDQGLIPFKIAAFDHGVNVTLGLPILRTSPDHGTAFDLAWQGTASPSSLFSAIRLAVR
                     MARNRMPPCPSVS"
     gene            complement(2755..3117)
                     /locus_tag="PK576_09200"
     CDS             complement(2755..3117)
                     /locus_tag="PK576_09200"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HOE61805.1"
                     /translation="MRIKAKEPVKKVVKAVAKGKDKPVQTKTRRTAPKTQRILFSVRA
                     EVGSQVSLAGSFNNWDPTAKPMTDKKGDGVYTVTVSLAPGTYEYKFVIDGTWCADPDC
                     ADWVQNEHGTLNSVKYVR"
     gene            complement(3312..4301)
                     /locus_tag="PK576_09205"
     CDS             complement(3312..4301)
                     /locus_tag="PK576_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOE61806.1"
                     /translation="MRIGLTYDLRADYLALGLSLEETAEFDKEETIAGIEDALRALGH
                     TTERIGHARALIRRLEAGERWNLVFNICEGLRGVAREAQVPAILELYEVPYVFSDPLV
                     LALTLHKGMTKRVVRDAGIPTSDFLVYETDEDLAELRFGAPFFVKPVAEGTGKGCSAK
                     SVVRERAALAAVCDDLVARFRQPALIEPYLPGREFTVGITGTGRQAAVAGSMEIVLLP
                     HAEAGGYSFDNKGNYEDRVVYPATDPARDPVAAMAERIALDAWRALGCRDGGRVDLRC
                     DASGRPQFLEVNPLAGLNPKDSDLPILARQCGITYTELIGRILDSAIQRQAGG"
     gene            complement(4337..5614)
                     /locus_tag="PK576_09210"
     CDS             complement(4337..5614)
                     /locus_tag="PK576_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOE61807.1"
                     /translation="MQLSFHLDDQEEEPPQGASPLFSLSVPFTDKKPELTHLPEPPVK
                     SRGLRMSPCCRAFRNRFFADATDLQWSDWRWQIQHRIRDLETVRRMIRLTAAEEKALE
                     LGGKMLPMAVTPHYMGLISPDDPEQAIRRAMIPTVYELRHCPGEAEDPLGEEHQSPIP
                     GLVHRYPDRVLLLATDFCSAYCRYCTRSRVVGHGAITPSTRRLKMIFEYIRERPQIRD
                     VLVSGGDPLTLSDERLEFILSNLRAIPHVEILRLGTKVPAVLPQRVTPRLTRMLRKYH
                     PLWMSLHFMHPDECGVETSRACGMLADAGIPLGSQTVLLRGMNDDPDVMRLLMHKLMK
                     MRVKPYYIYQCDPITGSEHFRTPVAKGVEIIRSLRGHTSGYAVPTYVIDAPGGGGKIP
                     VAPEYTAGRDAEGNLNLINYEGGSYRYPDFIPG"
BASE COUNT         1118 a         1668 c         1959 g         1261 t
ORIGIN      
        1 gataccggga gtatgtttct tgggttgtgc atcgcgacgt ttccgctgat gaccggatcg
       61 cgtaaagagt tggcggcctc gctgggcatg ccgttgctcg tcatgggggt gccgcttttc
      121 gacacgctgc tggccatctg gcggcgttcg gtgagggcgt gtctgccgag cgggttggtg
      181 gaggtcggca aacgggtgag ggtcatgcag ccggatcagg atcatgtgca tcaccggctg
      241 ttgcggcaga ccatgaatca gaagacagcc gcgatcattc tgtatgtgat cagcggagtg
      301 ttggtgttga tcggtttggt cggaaccatg ttgaaaggac gcgcgccggg gttgtttctg
      361 attgcgtttg tggtggcggt ttatattgtg gtccggcatt tggaacgggt ggagttgtgg
      421 gatacggggc ggttgctttc aggtcggcac gggatgatgc ggcaggggtt gcttatcccg
      481 ctctatatcg tttgggatgt gtttgccctg tgcggcacgt ggatggtggt gcggtgggag
      541 tcggggctgt cccttaaccg ggccgccgtg ttgtcggagt tgccggtttt catcgtgccg
      601 gtttttatgg cgctggtcgc gaccaaaacg tatgcgcgcg tatggggacg cgcacagatc
      661 cgcgatttcg tggtcttggg cttgtcggtt ttggcgggga ctgcgattgg ggcgggattg
      721 atctggttgc ttaatgatta cgggtgcggg atgatccggt tcgccgtccg gttcggtacg
      781 ctggcgattt ttccgattgc gggtgtccgg gcttggcgcg aatgcgtgcg cggggtcatg
      841 cagatgctgg agcgccgggt tctgctggaa aacccgtctg ccttgcgctt cctcgcgtac
      901 ggggggggat tgcggttccg cgcgtatctg cgcgagctga tggagcgacc gggcaaccat
      961 gaccgggtca tcatcgggat ccttgacgac gaggtgaatt tgaaagggcg gatcatcgcg
     1021 gggtatcccg tgctggggga ttttgaaacg gtccggtcct atgcggccga caattcgatc
     1081 aaaggggtga tcatcacctg tctgttgcct gaagcgaagt tacggtgggt gactgcggaa
     1141 ctggaggcga tggggctggt ggtttcggtt tgggcatgtg gggaacgggt gctggccaac
     1201 ccgaaggggt tggtcgtgcg ggaaatcgga aagggggaaa cgtgaagagt taccgtaaag
     1261 agttatggtt caagaccgac catcgccgtg aataccggaa cattacgccg gaagtagagg
     1321 cctgtctggc cgagagcgga atccaggaag ggttctgtct ggtcaatgcc atgcacatta
     1381 ccgccagtgt ttttgtgaat gatgacgagg cggggctgca tgccgatttt gagcgttggc
     1441 tcgaacggat cgcgccggag aagcctcaca gtcagtatgc gcacaacggg gctgaggata
     1501 atgcggatgc gcacctcaag cgtcaggtta tgggccggga agtggtggtg gcggtaaccc
     1561 gggggaaatt ggatttcggt ccgtgggaac agattttcta tggggaattt gacgggttgc
     1621 gttccaagcg ggtattggtg aagatcatcg gcgaatgaac gcgggatcag gaaacggaag
     1681 ggcaaggcgg catgcggttc cgcgccatgc ggacggcaag tcggatggcg ctgaacaggc
     1741 tggaggggct ggcggttccc tgccaggcaa ggtcaaacgc tgttccgtgg tcgggcgaag
     1801 tgcggaggat cgggagcccc agcgttacgt tgacaccgtg atcgaaggcg gcgatcttaa
     1861 aagggatcag cccttggtca tggtacatgg caacgtaggc gtcatacggg gcggattgcg
     1921 gcggggttga caagagccat gtaaaagcgg tgtcggcaac gagcgggccg gaggcgtgaa
     1981 tgccttcggc ccgcgctgtt tcaatagccg gcatgaggac gcgacgctcc tcggttccga
     2041 gcaggccgtt ttcgccggca tgcggattga gcgccaacac gccgagtcgc ggtgcggggg
     2101 tgccgaacat ccggcaggcc tcgtgcgtca gacgcagcgt acggagcagg gatgacacgg
     2161 tgagcaactc ggaaacacgc gccagcggct catggatggt cgccagacag acacaaaaag
     2221 ccggcgcata aaacaacatg cgcggatccg gggtctcggt cagttcgctc aggatctccg
     2281 tgtgcccgac cgcctgtact ccggcggcgt gcagggcgga tttgttgagc ggggctgtga
     2341 ccagagcgtg cgcgacctgg ttacgggaga tgtcgagggt tgcgcggcgc acccattcat
     2401 aagcctgacg accgcaagca gcctgaaccg ttccgggcgt gatcgagtcg gcttcggcaa
     2461 agggaaaatc aaaaagcacg tggccgtcct gtggcaaagg cccttccgcg ccatcaagga
     2521 ttttgatcgt cgcggcgaag gggatgccac tggcgacggc cacccgctcc agaagagtcc
     2581 ggttcccgta gacgagcagt tggcatgttc gggagagttc ctcatcagcc aacgcccgca
     2641 gagcaagttc tggtcccacg ccggcgccgt cacccaaagt cagggcgcaa cgtattctgg
     2701 cgtccgccat acgtcgtctc ctgccgtgtg cctcgcgcaa agcggggtat ccgcctacct
     2761 gacgtatttc acactgttga gcgtgccgtg ttcattctgc acccaatcgg cgcagtccgg
     2821 atccgcacac caggtgccgt caatcacgaa cttgtattcg tatgttcccg gcgcgagcga
     2881 cacggttacg gtgtaaaccc cgtcaccctt cttgtccgtc atgggtttgg cggtcgggtc
     2941 ccaattgttg aaactgcccg caagggaaac ctggctgccg acttcggcgc gcacgctaaa
     3001 cagaatgcgc tgggttttcg gcgcggtgcg ccgggtcttg gtttgcaccg gcttgtcctt
     3061 ccctttcgct acggctttca ccactttttt tacgggttct tttgctttga tacgcatatg
     3121 attcatcctt tttttgggtt gaaggcgatt aagtatacag aatcgggtgc gacctgtcta
     3181 gtaccaccct tttcacgcgc gattctgttt ttgaccattc ggaatccggt tcgggggggg
     3241 ccttcccaag ctgcaaagac gccccattcc cacgggagga acggggtgcc gtcccgtccg
     3301 ctttgagcct gttatccgcc cgcttgccgc tgaatcgccg aatccagaat acgtccgatc
     3361 agctcggtat aggtgatgcc gcactgacgc gcgaggatcg gcaggtccga atccttgggg
     3421 ttcagtcctg ccagcgggtt gacctccaga aactgggggc ggcccgatgc gtcgcagcga
     3481 agatccaccc ggccgccgtc gcggcagccc aacgcgcgcc aggcgtcgag cgcgatacgt
     3541 tccgccattg ccgcgaccgg atcccgcgcg ggatcggtgg cgggatagac cacgcgatcc
     3601 tcatagtttc ctttgttgtc gaacgagtag cctcccgctt cggcatgcgg caacagcacg
     3661 atctccatgg agcccgccac cgccgcttga cggcccgtgc ccgtgatgcc gactgtgaat
     3721 tcgcggccgg gaagatacgg ttcgatcaat gcgggctgac ggaagcgcgc caccagatcg
     3781 tcacagaccg ccgccaatgc ggcacgctcc cgcacaaccg acttggcgga acagcctttt
     3841 ccggtgccct cggccacggg tttcacaaag aacggcgcac cgaaccgcag ttccgcgaga
     3901 tcctcatccg tctcgtagac gaggaaatcg ctggtcggta tgccggcatc ccggaccacg
     3961 cgcttggtca tgcccttgtg cagggtcagc gcaaggacaa gcgggtcgga aaagacatac
     4021 ggcacctcgt agagttcgag gatcgccggt acctgagcct cgcgcgcgac gccgcgcagc
     4081 ccttcgcaga tgttgaagac cagattccag cgctctccgg cctccagccg ccggatcaat
     4141 gcccgggcat gaccgatccg ctccgtggta tgaccgagcg cacgtagggc atcctcgatc
     4201 ccggcgatgg tctcttcctt gtcaaattcg gcggtctctt ccagagacag gcccaaggca
     4261 agataatccg cgcgcaggtc atatgtcagg ccaatacgca taagggcatg tccgtatttc
     4321 ctgtccaaca ggtatctcaa cctggaatga aatcgggata ccggtaggaa ccgccctcat
     4381 aattgatcag gttcaggttc ccctcggcat cccggcccgc cgtgtattct ggcgcgaccg
     4441 ggatcttccc tcccccgccc ggcgcgtcga tcacgtaggt cggcaccgca tacccactgg
     4501 tgtggccacg caggctgcgg ataatctcca cacctttcgc caccggcgtg cggaaatgct
     4561 ccgaaccggt gatcggatca cactggtaga tgtaatacgg tttgacgcgc atcttcatga
     4621 gtttgtgcat cagaaggcgc atcacgtcgg gatcatcatt cattccccgg agcagaaccg
     4681 tctgcgaacc gagcgggatc ccggcgtcgg ccagcattcc gcaggctcta ctggtctcaa
     4741 ccccgcactc gtcggggtgc atgaaatgca gactcatcca gagcgggtgg tatttgcgca
     4801 acatgcgtgt gagccgcggc gtgacacgct gcggcaggac tgcgggcacc ttggtgccga
     4861 ggcggaggat ctcgacatgc gggatcgccc gcagattcga aaggatgaat tcgaggcgct
     4921 catcgctcaa ggtcagcgga tcaccccctg aaaccaggac atcgcgaatc tgaggacgct
     4981 cgcggatata ctcgaaaatc atcttgagcc gccgggtact gggtgtgatc gcgccgtgcc
     5041 cgaccactcg ggagcgtgtg cagtaccggc agtacgctga acagaagtcg gtcgccaaca
     5101 gcagcacccg gtcgggatac cggtgaacca gcccggggat cgggctctga tgctcctcgc
     5161 ccagcggatc ctccgcctcg ccggggcaat gccgcagctc atacaccgtg gggatcatgg
     5221 cgcgccggat cgcctgctcc ggatcgtccg gcgagatcag ccccatatag tggggcgtga
     5281 cggccatcgg caacatcttg ccgcccaact ccagcgcctt ctcctctgcg gcggtaaggc
     5341 ggatcatgcg gcgtacggtt tcgaggtccc gaatccggtg ttggatctgc caacgccagt
     5401 ccgaccactg gaggtcggtc gcatcggcga aaaaccggtt gcgaaacgcg cggcagcagg
     5461 gactcatccg gagcccccga cttttgaccg gcggttcggg aagatgcgtc aattccggtt
     5521 ttttgtccgt aaacgggaca gataaggaaa aaagaggcga cgctccttgt ggaggttcct
     5581 cttcctggtc atccaggtga aagctcaact gcatcgcgtg cttaggcaca ccgtacacag
     5641 caaactccta aacaaaaata ggcctataac cctcgtcacg tcacagcgcg acgcaaggag
     5701 cctctttgtg gtttcctaat acgcttgttt gggaagcttg tcaagcatgc ttagattgcg
     5761 gatccgtttt ttttgtgacc ggtaagggtt tctggtttca gggtttcagg gtttctggtt
     5821 tctggtttca gggtttctgg tttcatggtt tctgggtttc atccaaatcg gggtcgggat
     5881 cgctatcggg atcgggatcg ggaacgtcga cccgcgaccc gcgacattcg acccgcgacg
     5941 atcgacaggg acgcccgcgg acgccttaaa aagagcgttg aaagcaacgc tctttggttt
     6001 cagggt
//
LOCUS       DAOSLE010000156         5990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_11952_length_5990_cov_5.484246, whole genome shotgun sequence.
ACCESSION   DAOSLE010000156 DAOSLE010000000
VERSION     DAOSLE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11952_length_5990_cov_5.484246"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..673)
                     /gene="rdgB"
                     /locus_tag="PK576_09215"
     CDS             complement(62..673)
                     /gene="rdgB"
                     /locus_tag="PK576_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOE61808.1"
                     /translation="MKGVIATRNHHKLQEIRAILSVPSWEWVALDEVPGMPDEVVEDA
                     ATFEGNALKKAREVCQVSGLWTLADDSGLEVAALQQAPGVFSARYAGESCDSRANNAK
                     LLRELAGVTDRRARFRCVIALCAPDGREWTVEGCCAGRIVESERGVNGFGYDPLFVPD
                     GYALTFAELDSVTKNRISHRGRALRLAAREWSESVLGQVKPAT"
     gene            complement(686..1279)
                     /locus_tag="PK576_09220"
     CDS             complement(686..1279)
                     /locus_tag="PK576_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE61809.1"
                     /translation="MTPAYTEARKACFTVLDFETTGSVPGYAVEPWQVGLVRVRGGRV
                     CPDEAYESLLRIGDRPFNPRAPGRHAMLREVLRDAPALAEVWPDLSGWISGVPLVAHH
                     VGTERSVLRRFAPLHPLGPWVDTLVLVRWAYPSLRSKALADVVDALGLSGRVRELCPD
                     RDAHDALFDAFACAAVLEHLLGLPGWEQITVQALAGR"
     gene            complement(1401..2231)
                     /locus_tag="PK576_09225"
     CDS             complement(1401..2231)
                     /locus_tag="PK576_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61810.1"
                     /translation="MRQRFWIIMGLLAAGGAVALGLCLPRFFEGSMPQVPGEDVLHLV
                     LGDARQELSMALLAKADEYFHGGVNDVACGHGMTSVEGAVPDHDPAHGESCPEHAHAH
                     EAVRWRDPWAWINGRVHVQEHRHLCNDQATELLPWIWAACRASPQNAQAYQTGAYVLA
                     RMTGHPEQGVRLLEEGHRRNPEDAGIVFSLGEMWLTHLHDAARAESWFAAVLPLCRPA
                     EGAVGENDRLLWVRALFYLGYLAKQRGDLARARACLAEAEAVFPEHASVKDLRRLLQQ
                     "
     gene            complement(2239..3021)
                     /locus_tag="PK576_09230"
     CDS             complement(2239..3021)
                     /locus_tag="PK576_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE61811.1"
                     /translation="MKMCGRQTGALVRVALLDLYRRKDLIVVWLLSLVLLLPLAFFSP
                     FGISGAGRYLNEIALLLVWLFSAVIGVGVAARLFPAEFESRTIFPLLAKPVTRGRIVL
                     GKYLGALAASVSALTVFYLAYGVLCGLRQSVWFPVVLFQAFLLHIGFLIVVTALAMLG
                     SLMMTPSANLTLCGLLIVGMLVFGQRLPSLIAAQPYPGKAFLMLVHALGPHVEFFDLR
                     QRVIHGWSRVGWDVCAVVLLYAIGYAAACLGLAGWLFCRKKV"
     gene            complement(3018..3761)
                     /locus_tag="PK576_09235"
     CDS             complement(3018..3761)
                     /locus_tag="PK576_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE61812.1"
                     /translation="MRSGDTMDLLTLEGVTKTFGDVRAVDGLTLSIRQGEILGFLGTN
                     GAGKTTTIKMLLGFFRPDSGKIAIWGGDPRTGAVRRRIGYMPETACYYPFLTIRELLD
                     FYGGLCGMTRADIRSRAEQLIGRVGLAGVEKRLLRHYSKGMLQRAGIAQALLHDPDLL
                     ILDEPFTGLDPLARIQLRDLIRELRAQGKTIFFSSHELSEAELICDRVAMIRRGQTVW
                     CGALHEVAGDGQQNLERIFLKMLGGEETA"
     gene            complement(3768..5309)
                     /gene="amrB"
                     /locus_tag="PK576_09240"
     CDS             complement(3768..5309)
                     /gene="amrB"
                     /locus_tag="PK576_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE61813.1"
                     /translation="MRRMLSGFCAMLLCAGAGCQRGGPVPEEAEMTKKVMASPLAGRW
                     YQDSERALRDEIATCCKDIMVVRKKSVCAAVVPHAGYRYSGRVAAGVYMRIDPSRLSR
                     VVVMGPSHYTAMRNQISVPDATHFATPLGELSADIGFLRRLRALPFVTHVPEAHTQEH
                     SDQIQLPLIQACLSKELPVVCMICGQFDAAGLLDAAAALRPLLDEGTLVVASSDFTHY
                     GSSYGYVPFTRDVDRNIETLDMGIFELFAQKDLQGFLERLRETGATVCGRDPLALLLA
                     MLPQEAKVERTGYDTSGRMTGDTRQSVSYVGAVVTGKWAPATPRAAPLAADSPIPRED
                     CDRLLALARDTLTRALRDGERRAAIAEPPKLTEGMLAVRGGFVTLNKRGALRGCIGEI
                     VPRREIWKVVREQALNAAFHDPRFNPLEATELAEIEIEISMLTPPKPVKSWREIVVGK
                     HGMVLNKEGHSAVFLPQVAPEQGWGIEETLTHLSRKAGLPSNAWREGATFLVFEAQVI
                     REKGR"
     gene            complement(5315..5470)
                     /locus_tag="PK576_09245"
     CDS             complement(5315..5470)
                     /locus_tag="PK576_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61814.1"
                     /translation="MTRPGGVRLRYARRDFLFGAVAMLGCGCRWRGARVLPLQASRIR
                     QMAKWAG"
     gene            complement(5467..>5990)
                     /locus_tag="PK576_09250"
     CDS             complement(5467..>5990)
                     /locus_tag="PK576_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE61815.1"
                     /translation="PLRALAPLVDAVNVDIKAFQDEFYRNVCDGTLEPVLRSVLLFKE
                     AGTHVELTNLLIPTLNDSDAFLDPLCEWIRDRVGAETPMHFSRFFPSFRMTRLPPTPI
                     ETLLHARDRAHAAGLRHVYVGNAVVPEGENTRCAGCGETVVRRQRYVVTENRLTPQGA
                     CPACGRTAYGVWS"
BASE COUNT         1290 a         2153 c         1596 g          951 t
ORIGIN      
        1 tttggatgaa accttgaaac caaagagagt tgaaggttca actgctcttt ttaggctgaa
       61 gtcaggtcgc gggcttgacc tgccctaaaa ccgattcgct ccattcgcgg gccgccaacc
      121 ggagcgcgcg gccgcggtgt gaaatccggt tctttgtgac actgtccagt tcggcaaagg
      181 tgagcgcata cccgtccggc acgaacagcg ggtcatagcc gaacccgttg acaccgcgct
      241 ctgactccac gatgcggccg gcacaacatc cctccacagt ccactcccgc ccgtccggtg
      301 cgcacagcgc gatgacgcag cggaatcggg cgcgccggtc cgtaacgcca gccagttccc
      361 gcaacagctt ggcattgttg gcgcgggagt cacaggattc accggcatag cgtgcggaaa
      421 agacacccgg cgcctgttga agtgcagcca cctccagacc ggaatcgtcc gccagcgtcc
      481 acaagcccga gacctggcag acctcacgcg cttttttcag cgcattgccc tcaaacgtgg
      541 ccgcatcctc aaccacctcg tccggcatac cgggcacctc atccaacgct acccactccc
      601 atgagggaac agacagaatc gcgcggatct cctgaagttt atgatggttc cgtgttgcga
      661 tcaccccttt catcgtcttc ccccttcatc tgcccgcaag ggcctgaacc gtgatctgtt
      721 cccagccggg caacccgagc aggtgttcga gcaccgcagc acaggcgaac gcgtcaaaca
      781 acgcgtcatg cgcgtcacgg tcgggacaca gttcccggac ccgtccggaa agcccgaggg
      841 catcgaccac atcggccaag gcctttgatc tcagcgacgg atatgcccag cgcaccagca
      901 ccaaggtatc cacccacggc cccaacgggt gcagcggcgc aaagcgcctc agcacactgc
      961 gttccgtacc gacgtgatgc gccaccagcg gcacgccgga gatccagccg ctgagatccg
     1021 gccacacctc agcgagcgcg ggcgcatcac gcaaaacctc acgcaacatg gcatgccgac
     1081 ccggcgcgcg cgggttgaag ggccggtcgc caatccgcaa caggctctca tacgcctcat
     1141 ccggacaaac ccgcccaccg cgcacgcgaa ccagtcccac ctgccaaggc tcgaccgcat
     1201 accccggcac cgaaccggtg gtttcaaaat ccaacacggt gaagcaggcc ttccgggctt
     1261 ctgtgtacgc gggcgtcatg cgtcagcgca tgttcccgag ttccgcatga atgcacccgc
     1321 acgcggcgag caacaccagc agcgcacacg ccacaacaca tccgaacccg attgtccgca
     1381 tcgtcatccg catccccttt ctattgctgc aacaaacgcc gcagatcctt cacactcgca
     1441 tgttccggaa aaaccgcctc ggcctccgcg agacaggccc gcgcacgcgc aagatcaccc
     1501 cgttgtttcg cgagataccc gagataaaaa agcgcacgaa cccagagaag ccggtcgttc
     1561 tcgccgaccg caccctcagc gggacggcaa agggggagca ctgcggcgaa ccaggattcc
     1621 gcgcgcgccg catcgtgcag gtgtgtgagc cacatctcgc cgagcgagaa gacgataccc
     1681 gcatcctcgg ggttacggcg gtggccctct tccaacaggc gcacgccctg ctcggggtga
     1741 ccggtcatgc gggccagcac ataggcaccg gtctgatacg cctgagcatt ctgcggcgaa
     1801 gcacggcagg cggcccagat ccacggcaga agttccgtcg cctgatcgtt gcaaagatgg
     1861 cggtgttcct gcacatggac acgtccgttg atccacgccc acggatcacg ccagcgcacc
     1921 gcttcatgcg catgagcgtg ttcggggcac gactccccgt gtgcgggatc atgatccgga
     1981 accgctccct cgacagaggt catgccgtgc ccgcacgcga catcgttcac tccgccgtga
     2041 aaatactcgt cggctttggc cagcagcgcc atgctgagtt cctgacgggc atcgcccaac
     2101 acaaggtgca ggacatcctc acccggcacc tgcggcatcg acccctcgaa aaaccgggga
     2161 agacataaac cgagcgcgac cgctcctccc gcagccagta accccatgat gatccagaaa
     2221 cgttgacgca tggccctttt acactttctt cctgcaaaac aaccatcctg ccaacccgag
     2281 acaagccgcg gcataaccga tcgcgtacag cagcaccacg gcacacacat cccaccccac
     2341 acgcgaccac ccgtggatca cgcgctggcg caaatcaaaa aactccacat gcggcccgag
     2401 ggcatgcacc agcatcagga acgccttgcc gggatagggt tgcgccgcga tcaaggacgg
     2461 caggcgctgc ccgaaaacga gcattcccac aatcaacaac ccgcacagcg tcagattggc
     2521 ggagggggtc atcatcaggg aacccagcat cgcgagtgcc gtcaccacga tcagaaagcc
     2581 gatatgcagc aggaaagcct gaaacagcac caccgggaac cagacgctct gacgcagacc
     2641 gcacagcact ccgtacgcca gataaaacac cgtcagtgcc gaaaccgagg cagccaacgc
     2701 gcccagatat ttccccagca cgatcctgcc acgcgtcacc ggcttggcca acaacggaaa
     2761 gatcgtccgg ctttcgaatt cggcggggaa gaggcgcgcc gccacaccca ccccgatcac
     2821 ggccgaaaac agccagacca gcagcaaggc gatctcattc agatatcttc ccgcaccaga
     2881 gataccgaac ggcgaaaaga acgccagcgg caaaaggagc accagactga gcaaccagac
     2941 cacgatcagg tccttgcgcc ggtacaaatc caacagcgcc acacgcacca gcgctcccgt
     3001 ctgacgaccg cacatcttca tgccgtctcc tcccccccaa gcatcttgag aaagatacgc
     3061 tccagattct gctgtccgtc acccgccacc tcatgcagcg caccgcacca gaccgtctga
     3121 ccgcgccgga tcatcgccac ccggtcacag atcaactccg cctccgagag ttcatgcgaa
     3181 gagaaaaaga tcgtcttgcc ctgcgcccgc aactcccgga tcaggtcgcg gagttgaatc
     3241 ctcgccagcg ggtcaagtcc ggtgaacggt tcatccagaa tcaacagatc cggatcatgc
     3301 agcaaagcct gagcgatacc cgcacgctgc aacatgcctt ttgagtagtg ccgcaacagg
     3361 cgtttttcaa ccccggccaa ccccacccgc ccgatcagtt gttccgcgcg cgaccggatg
     3421 tcggcccggg tcatgccgca caacccgcca tagaagtcca gcagttcccg gattgtcaga
     3481 aaggggtagt aacaggcggt ttccggcatg taaccgatcc gtcgccggac agcgcccgtt
     3541 ctggggtcac cgccccaaat cgcgattttt ccggagtccg gacggaagaa gccgagcagc
     3601 attttgatgg tggtggtctt ccccgcgccg ttcgtgccga gaaatcccag aatctcgccc
     3661 tgccggatcg acagcgttag cccgtccacg gcacgcacgt caccgaacgt tttcgtgacc
     3721 ccctccagcg tgagcagatc catcgtgtct ccactccgca tgacttttta cctccccttt
     3781 tcccgaatca cctgagcctc gaacacaagg aacgtagccc cttcgcgcca ggcattcgac
     3841 ggcagacccg cctttcggga aagatgcgtc agcgtctctt cgatccccca accctgttcc
     3901 ggcgcgacct gcggcaggaa caccgcgctg tggccctcct tgttcaacac cataccgtgc
     3961 ttcccgacaa caatctcccg ccaggatttc accggcttcg gtggagtgag catcgagatt
     4021 tcaatctcga tctcggccag ctccgtcgct tcgagggggt tgaaacgtgg atcatgaaac
     4081 gccgcgttca acgcctgctc gcgtaccacc ttccagattt cgcgccgggg cacgatctcg
     4141 ccgatgcatc cccgcaaggc gccgcgtttg ttcagcgtca cgaaacctcc gcgtaccgca
     4201 agcatcccct cggtcaactt cggcggctcc gcaatggccg cacggcgctc gccgtctcgc
     4261 agcgcccgcg tcagcgtgtc gcgcgccaaa gccagcagac ggtcgcaatc ctcacgagga
     4321 atcggactgt ccgccgcaag cggtgccgcg cgcggcgtgg ccggcgccca cttgccagtc
     4381 accaccgccc ccacatagct caccgattgg cgggtatcgc ccgtcatcct gccggaggtg
     4441 tcatacccgg tgcgctccac cttggcctcc tgcggcagca tggccaacag caaggcgagc
     4501 ggatcgcgcc cgcacaccgt ggcgcccgtc tcacgcaatc gctccaaaaa cccctgcaag
     4561 tccttttgcg cgaacagttc gaaaatcccc atatccaatg tttcaatgtt gcggtccaca
     4621 tcgcgcgtga agggcacata accgtaactc gaaccatagt gggtgaaatc ggaactcgcc
     4681 accaccagcg ttccttcgtc caacaacgga cgcagagccg ccgccgcgtc gagcaggccg
     4741 gccgcatcga actggccgca gatcatgcac acgaccggaa gttccttcga caggcacgcc
     4801 tgaatgagcg gcagttgaat ctgatcgctg tgctcctgcg tatgggcctc cgggacatgc
     4861 gtcacgaaag gcagggcgcg aagccgacgg aggaacccga tatccgcaga caactcgccg
     4921 agcggggtcg cgaaatgcgt cgcatccggc acactgatct gattccgcat cgcggtataa
     4981 tggctcggcc ccatcaccac cacccggctc agccgggacg ggtcaatccg catatacacg
     5041 cccgccgcca cacgtccgga atagcggtat cccgcatgcg gcaccaccgc cgcgcacacc
     5101 gattttttac gcacgaccat gatgtccttg caacatgtgg cgatctcatc ccgcaaggcc
     5161 cgctcactgt cctgatacca acgccccgcc aacggcgagg ccatcacttt tttcgtcatc
     5221 tccgcctcct ccggcaccgg gccgccacgc tgacaccccg cgcccgcgca caacaacatc
     5281 gcacagaaac cagacaacat gcgtctcatc cgcatcaccc cgcccattta gccatctgac
     5341 ggatccgcga ggcctgcaac ggcagaactc gcgcgccgcg ccaccggcat ccacacccca
     5401 gcatcgcgac cgcaccgaac aagaagtccc gacgcgcgta ccggagtcgc actcctcccg
     5461 gccgcgtcat gaccagactc cgtaagccgt ccttccgcat gcgggacaag ccccctgcgg
     5521 cgtcagacgg ttctctgtca ccacataccg ctgccgccgc accaccgttt cgccgcagcc
     5581 cgcgcaacgg gtgttctccc cctcgggcac cacggcgttt ccgacataca catgccggag
     5641 tcccgcagcg tgtgcgcggt cgcgggcgtg cagcaaggtc tcgatcggcg tgggaggcaa
     5701 ccgcgtcata cggaaactcg ggaagaaacg ggagaaatgc atgggggtct ccgcgccgac
     5761 ccggtcgcgg atccactcgc agagcggatc cagaaacgca tcgctgtcat tgagggtcgg
     5821 aatcagcaga ttggtgagtt cgacatgcgt gcccgcctct ttaaacaaca gcacgctccg
     5881 gagcaccggc tccagtgtgc catcgcacac gttgcggtaa aactcatcct gaaaggcttt
     5941 gatgtccaca ttcacggcgt ccacgagcgg tgccagcgcg cgcaacgggg
//
LOCUS       DAOSLE010000157         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12143_length_5920_cov_8.752600, whole genome shotgun sequence.
ACCESSION   DAOSLE010000157 DAOSLE010000000
VERSION     DAOSLE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12143_length_5920_cov_8.752600"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /locus_tag="PK576_09255"
     CDS             complement(<1..2297)
                     /locus_tag="PK576_09255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE61816.1"
                     /translation="MDETLKERIGDYELLELIGDGAQGRVYKARHMAGPEGEPQELVA
                     LKVVRITGEDEKLRLRFQEQAEILRRLNHAHIVAYRDCFTWHAGEWDEAQCLVMELLE
                     GETLAELLKHASSGLPWPQVEQIFEQCLAGLIHARECGITHRDIKPSNIFLTKGGQAK
                     IIDFDIARRDDSGQMSTAGWKGTFDYMAPDFISIDGFRGDETSDVFSLGICFYQALTG
                     SLPYGKLGEAAHIGYLNRWRDQAAPVPSFRPGVFRVLAHAKTVVSKAIAARREQRYAT
                     FAAFLEEFRMIRYRRIRNKNKDEYELLAVLGRGGFGEVFKARRVSDGLVVAVKFLFSE
                     KQSDRFLKEAKILQQYPHPNLVQYVDFIVIKGVTGEKQFFLVMEFLEGMPGWTLRVRL
                     KNEGKLEVEEAVTLFNCYLSALQFLHGSARPIIHRDIKPGNLYAPVGQPEKGKVFDLG
                     VARDVSGTVTVGGVPGTLDYMAPEFADAGGDRGSPQSDLYALGLCLYEVLAGKPVFER
                     LPGDLNSAWVAFQDRLRKPPEIAFDAEVFVRYPRFKGVILRALALKPDDRFPSADAMR
                     KALQEALLSGSAPQADEGFDADEGLTMATFVGARPAEPEPGATTGTRAVSSEVLQEAL
                     DEQARQADRRPRERAGRRGPGYLIAAIAGTAVTMTLLFGLVVYWLGREKPKAGQTARV
                     EKPATPTASAPAETKPAPAPEVAAAKPAAVAQPAEPSEPARQPEAKPAPAPEVAAAKP
                     AAVAQPAEPSEPARQPEAKPAPAPEV"
     gene            complement(2325..3446)
                     /locus_tag="PK576_09260"
     CDS             complement(2325..3446)
                     /locus_tag="PK576_09260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOE61817.1"
                     /translation="MDTPFSYLESAAVTDVGRRRKNNEDALVELPACGVFCVADGMGG
                     AKGGEIASQATVEALQTVFEHSTDAPFAVTAAAAARLAARALNQASAWIRENAEERGL
                     TGCGSTAVMLLFDRINPACARVLHAGDSRAYRLRGGRLEQLSQDHSVAAAAGLKDEKD
                     LPAMFRGVITRAVGLEKSVRLEETLVEVHGADLYLLCSDGLSKMVSDRHIQKLLRKHA
                     TEPIGTLAQRLIDEALHAGGEDNISVILVRVADKLPPATVTELSPETLALEALDLDGV
                     PAAADSEDSEPLTRDTRETADTAGCECAKTPRDIDTDQAIQTPPTPFSGTGITPEENV
                     EKEEVAPKPRRRLLLRILVVCLMAGLAAAVWILFRTFRP"
     gene            complement(3467..5383)
                     /locus_tag="PK576_09265"
     CDS             complement(3467..5383)
                     /locus_tag="PK576_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE61818.1"
                     /translation="MEKAGRDGARARSWSVWLVTVGIGLAAGCALLDSGPSVPGGAPA
                     QTVDFGTPEGVQVSSDGLREAVVTWAPPTVKAYRYRIERSPAADGPFVMVAEVPPKAL
                     SYADGADPSSRLEDATAYHYRVIAVLERNGPVSPPSAVVRSVTAPPPVPPEAIRAEAT
                     GSRAVTVTWQASGSIGVTGYRVERAPVDEPTAFAPVSGEVSTLTFTDGGKPSSTLKDS
                     SEYLYRVLTVNRAGSSSAPSATARVKTLPRPAQVKGLKAVSREVRCVPLKWTPSPEAD
                     VTHYTVFRARSSEGPFEQIGTAQGRAGAEYTDGGANPGNLEDEGTYFYFVRAVNVVTA
                     ESIDSDTVRAVTREVPPEVKQVAAVSARPREVPVEWEASPDTAVVGYEVWRTLAGADD
                     WTQVGRVNSRETTRFLDRGGEQDATRLGKLQDGTEYQYRVVAFNTATVRSSASVPAPA
                     KTKVIPVPPSGLVATTNLPHAIRLTWSPNPEPDVDGYRLEASKKPDGGFRRWVEFRLN
                     EKTDLSAEEKELDPSQVRYYRVMALDREGLESLWSEPIEGRAKPLPDPPFDLQAETEG
                     ALRRITWQPPPQPDVVRYVVWEKWFLGWKRLADTDKPEFTLTLPPDQKPPTLAVSAVD
                     QDGLESRRSEAVNP"
BASE COUNT         1099 a         2006 c         1760 g         1055 t
ORIGIN      
        1 acctccgggg ccggcgcggg cttcgcttcg ggctgccggg ccggttccga aggctcggcc
       61 ggctgcgcga cggcagcggg tttcgcggcg gcaacctccg gggccggagc gggcttcgct
      121 tcgggctgcc gggccggttc cgaaggctcg gccggctgcg cgacggcagc gggtttcgcg
      181 gcggcaacct ccggggccgg cgcgggcttc gtttctgctg gggctgatgc ggtcggcgta
      241 gccggtttct ccacacgcgc ggtctgaccg gctttaggct tctcccgccc cagccaataa
      301 acgacgagac cgaaaagaag ggtcatggta accgccgttc cggcgatcgc ggcgatcagg
      361 tagccggggc ctctccgacc cgcgcgctcg cgcggccgtc ggtccgcctg ccgcgcctgc
      421 tcatccagcg cttcctgaag cacctcggac gacacggcac gggttccggt tgtggcaccc
      481 ggctccggtt cggcaggccg cgccccgacg aaggtggcca tggtaagccc ttcgtccgcg
      541 tcaaacccct catccgcctg cggcgcgctc ccagagagta acgcctcctg cagagccttg
      601 cgcatcgcat cggcactggg gaaccggtcg tcgggtttca gggccagcgc gcgaaggatc
      661 acccctttga accgcgggta gcgcacgaac acctcggcgt cgaaagcaat ctccggcggt
      721 ttgcgcagcc ggtcctgaaa tgcgacccag gcggagttga ggtcacccgg cagacgttcg
      781 aacaccggct tgcccgccag cacctcatag aggcagagac cgagcgcata gaggtcggac
      841 tgaggcgaac cgcgatcccc gcccgcatcc gcgaactccg gggccatata atccaaagtg
      901 cccggcacgc caccgaccgt gaccgtaccc gagacatccc gcgccacgcc gaggtcaaag
      961 accttgccct tctccggttg cccgaccggc gcatagaggt tcccaggctt gatatcacgg
     1021 tggatgatgg ggcgcgcgct gccgtggagg aactgcaggg ccgagaggta acagttgaag
     1081 agcgtgaccg cctcctccac ttccagcttg ccctcgtttt taagtcgcac ccgcagggtc
     1141 catcccggca taccctccag aaactccatg accagaaaga actgcttctc gccggtgact
     1201 cccttgatga caatgaaatc gacatattgg accagattgg gatgcggata ctgctgcagg
     1261 atcttcgcct ctttgaggaa gcggtcggac tgcttctccg aaaagaggaa cttgacggcc
     1321 accaccaacc cgtcgctgac gcggcgcgct ttgaagacct caccgaaccc gccgcggcca
     1381 aggaccgcga ggagttcata ctcatcctta ttcttgtttc ggatccgacg gtagcggatc
     1441 atgcggaact cttcgaggaa ggccgcgaaa gtggcgtaac gttgttcgcg acgggcggcg
     1501 atggctttgg aaacaaccgt tttggcatgg gccagcacac ggaacacgcc gggccggaac
     1561 gacggcacgg gggcggcctg atcgcgccac cggttgagat atccgatatg cgcggcctca
     1621 cccagcttcc catacggcag agaaccggtg agcgcctgat agaagcagat gcccagactg
     1681 aacacatcgg aggtctcgtc accacggaac ccgtcgatcg agatgaaatc cggcgccatg
     1741 taatcaaacg tgcccttcca accggcggtg gacatctgtc cggaatcatc gcgccgcgcg
     1801 atatcgaagt cgatgatctt ggcttgaccg cccttggtga ggaaaatgtt ggaaggcttg
     1861 atgtcgcggt gggtgatgcc gcactcgcgc gcgtggatca gtccggcgag gcattgctcg
     1921 aagatctgtt ccacctgcgg ccaaggcagt ccggaagagg catgcttgag cagttccgcc
     1981 agcgtctcgc cttcgagcaa ttccataacc agacactggg cctcgtccca ctcgccggca
     2041 tgccaggtga agcaatcgcg gtaagccacg atatgggcat ggttgagccg acgcagaatc
     2101 tcggcctgct cctgaaacct gagccgcagc ttctcgtcct cgccggtgat acgcacgacc
     2161 ttgagcgcaa ccaactcttg cggttcgcct tccggaccgg ccatatgccg ggccttgtag
     2221 acccggccct gggcgccgtc tccaatcagc tccagcaatt cataatcgcc aatccgctct
     2281 ttgagggtct catccatgag gtacgggtcg ctccttcatc atgattaagg tcggaatgtc
     2341 cggaagagta tccacaccgc ggccgcgaga cccgccatga gacagacaac caaaatacgc
     2401 aacagcagac gacggcgcgg cttcggcgca acctcctcct tttccacatt ctcctccggc
     2461 gtaatgccgg tgccggaaaa gggcgtgggc ggggtttgta tcgcttgatc ggtgtcgatg
     2521 tctctgggcg ttttggcgca ttcgcagcct gcggtgtcgg cggtctcccg ggtgtcgcgc
     2581 gtgagcggct cggaatcttc agaatctgcg gcggcaggca caccatcgag gtcgagagct
     2641 tctaatgcga gcgtttccgg agacaactcc gtgaccgtcg ccggcggcag tttgtcggcc
     2701 acgcgcacga ggatcaccga gatgttgtcc tcgccgccgg catgcagggc ctcgtcgatt
     2761 aggcgttgcg ccaaagtccc gatcggctcc gtagcgtgct tgcgcagcaa cttttggata
     2821 tgacgatcgc tcaccatttt agaaagtccg tccgagcaga gcagatagag gtcagcgcca
     2881 tgcacctcga ccagcgtctc ctcgagccgc accgacttct ccaacccgac cgcgcgcgtg
     2941 atgaccccgc ggaacatggc gggcagatct ttctcatcct tcagtccggc ggcagcggcc
     3001 acggaatggt cttggctcaa ctgttcgagc cgtccgcccc gcaggcggta ggcgcggctg
     3061 tcacccgcat gcaacacacg ggcacacgcg gggttgatcc ggtcgaagag cagcatcacg
     3121 gcggtactgc cgcaaccggt caacccgcgc tcctcggcgt tctcccggat ccacgcgctt
     3181 gcctgattca aagcgcgcgc cgccagccgg gccgcggccg cggcggtcac ggcgaaaggc
     3241 gcgtccgtcg aatgctcgaa caccgtctgc agggcctcca ccgtagcctg gctggcgatc
     3301 tccccgccct tcgcgccgcc cataccgtcc gccacacaaa acactccgca ggcggggagt
     3361 tccaccagcg catcttcatt gttcttgcgt cgccggccga catccgtcac cgccgctgat
     3421 tcgaggtaag agaaaggtgt atccatggca ctcccggtta ctgaaattac ggattcaccg
     3481 cttcactgcg gcggctttcg agcccatcct gatccaccgc cgacacagcc aatgtgggcg
     3541 gtttctgatc cggcggcagc gtgagcgtaa actccggctt atcggtgtcg gccagccgct
     3601 tccaacccaa aaaccatttt tcccaaacaa cgtaccggac cacgtccggc tgcggcgggg
     3661 gctgccaagt gatgcgccgc aaggcacctt cggtctccgc ctgcaagtcg aacggcggat
     3721 ccggcaacgg tttggcccgg ccctcgatcg gctcgctcca caggctttcc aagccctcac
     3781 ggtccagtgc catcacacgg tagtagcgga cttgcgaggg atccagctct ttctcctccg
     3841 cgctcagatc cgtcttctcg ttcagcctga actccaccca tcgcctgaat cctccatccg
     3901 gttttttgga agcttcgaga cggtagccgt caacatccgg ctcgggattc ggactccaag
     3961 tcaaccggat cgcatgcggc agattggtgg ttgccacgag tccggagggc ggcaccggga
     4021 tcaccttggt cttggcgggc gcgggcacgg aggcggacga gcggaccgtg gccgtgttga
     4081 aggccaccac ccggtattgg tattcggtcc cgtcttgcag cttacccagg cgcgtggcat
     4141 cctgttcgcc gccccgatca agaaaacggg tcgtctcgcg actgttcacg cgacccacct
     4201 gagtccagtc gtcggccccg gcgagggtgc gccacacctc atacccgacc acagcggtat
     4261 cgggcgaggc ctcccactcg accggcacct cgcgcggccg cgccgatacg gcggcgacct
     4321 gcttgacctc gggcggcacc tcgcgggtca cagcccgcac cgtatcggaa tcgatactct
     4381 ccgcagtcac cacgttcacc gcccgaacga aatagaagta ggtgccctca tcctcgagat
     4441 taccgggatt ggccccgcca tcggtgtatt ccgccccggc gcgtccctgc gcggtgccga
     4501 tctgttcgaa gggaccctca gacgaacgcg cccggaacac ggtgtagtgc gtcacatcgg
     4561 cttcgggact cggcgtccac ttcaacggca cacagcgcac ctcgcgcgac acagccttaa
     4621 gccctttgac ctgcgcgggt cgcggcaggg tcttcacacg cgcggtggcg gacggcgcgc
     4681 tcgatgatcc ggcccggttg acggtaagaa cacggtacag atattcgcta ctgtccttga
     4741 gcgtcgaact gggcttgccg ccatccgtga acgtgagcgt actgacctcg cctgacaccg
     4801 gcgcgaacgc cgtgggctca tcaaccggcg cgcgctccac gcggtatccg gtcaccccga
     4861 tcgaacccga agcctgccac gtcaccgtca cggcgcggga accggtcgcc tcggcccgaa
     4921 tcgcttccgg cgggaccggc ggcggcgcgg tgacactccg caccacagcc gaaggcgggc
     4981 tgaccggacc gtttcgctcc aacactgcaa tcacgcggta atgataggcc gtggcatcct
     5041 ctagccgcga ggatggatca gccccgtccg catacgataa cgctttggga ggcacctcgg
     5101 caaccatgac gaacggaccg tcggctgcag gcgaacgctc gatccggtac cggtacgcct
     5161 tgaccgtcgg cggcgcccac gtcaccaccg cctcgcgcaa cccgtccgac gaaacctgca
     5221 ccccctcggg tgtgccgaaa tccacggtct gcgccggcgc gccacccggc acactcggcc
     5281 ccgaatccaa caaggcgcac cctgccgcca agccgatccc aaccgtgacc agccacacgc
     5341 tccacgaccg tgccctcgct ccgtcgcgtc ctgccttttc catatccggc ctccacacct
     5401 gaatcatggc atttgtcgga aagaatactc catcgacagg gtaaaatcca gtccgattca
     5461 cagaaaaggg agaaaaacgc cggggggagc ccgacgctct ttttgaaccc ttagtcccga
     5521 cgcttcgtcg ccccacttcg tcgcaagcgc aatcgactga gttttggaga ccggtgcgta
     5581 tgcgggacgc ccgcttctcc cgtcagacct tatgtcgact cgcgacccgc gacgagcgac
     5641 ctgcggtgtc ggaaacgcgg gcgtccctgt cgatcgtcgc gggtcgaatg tcgcgggtcg
     5701 cgggtcgacg ttcccgattc cgattccgat agcgattccg atcccgatac cgattccgat
     5761 agcgatcccg accccgaccc cgatttggat gaaaccctaa aaccttgaaa ccaaagaaag
     5821 agagttgaag gttcaactgc tctttttagg ctaatgcact aatgcactaa tacactaatg
     5881 aactaatgaa ctaatgcact gaaaatgcgt tgcgtatcag
//
LOCUS       DAOSLE010000158         5900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12192_length_5900_cov_8.186997, whole genome shotgun sequence.
ACCESSION   DAOSLE010000158 DAOSLE010000000
VERSION     DAOSLE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12192_length_5900_cov_8.186997"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..242)
                     /locus_tag="PK576_09270"
     CDS             complement(66..242)
                     /locus_tag="PK576_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61819.1"
                     /translation="MLVPFLSVFTGPPQPGLLPPTAIRASFRADRETKQQMLAAAGDE
                     TNYKRDKQYRRWSI"
     gene            complement(333..605)
                     /locus_tag="PK576_09275"
     CDS             complement(333..605)
                     /locus_tag="PK576_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61820.1"
                     /translation="MNKNTPHLVRHNRRRAGGVNMEYVILAVLIAAAVVVAVVVFSRS
                     VASMFIAAGEATTLREQDARENLNMRRGDRREDADIAKEYHDSMHK"
     gene            complement(619..1512)
                     /locus_tag="PK576_09280"
     CDS             complement(619..1512)
                     /locus_tag="PK576_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61821.1"
                     /translation="MKTSLAHLIQRFVFAILAVGLLPTGAASVAAATPQAAGGFPSGK
                     IKMADAPFVSLGPSLMQHSDVLAEAGKLGLKGADKVLDGLDAVQDSPYYLPADAGMSI
                     TKDLGEAITKALAEGKDIGELLDKHKDTLKGAGDKIKVITIVLKSLKVAHFTGHVIET
                     YNSGDRKLFTKALHNAVKEGLKMGGEFTGSWAGGALGAKAGAAIGVWFGGVGAVPGAI
                     IGGAVGSWVGGKTVETGAGWVTDTFFKDSIKDLADWAFDKKHGPEKKPKLPSPLPESS
                     SGTPGGADSSSQAPPKLKPFR"
     gene            complement(1509..2567)
                     /locus_tag="PK576_09285"
     CDS             complement(1509..2567)
                     /locus_tag="PK576_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61822.1"
                     /translation="MTILTAWTAFAGLGMVFMPSASGADNTGGKTSESEPTAEQVGAG
                     ILHGLTVNLFRLHAASDVDGAVKKWGTGAFQAKWTRDVREVFNEHETIALKDFFTSAV
                     VWVGGMKGPHGIVALYSPWSDALLVIEQRVEAGPSPKASFSDFRFVSGESLRGDSATI
                     TPEGSFSLHPLREPLTVAISRLYAPSVAMFTRLYPPEGAPELLPAALRDRIDSPVGEL
                     ILIKARLVLRLNMFQDYLSAENKGWMIQSGVLIRALTAGDPAALRKFLSEKQSPAIIE
                     TVCALPPETRKTFTPVYFSKARDSVIMGLVNPTAPRWFIEAEFLGAEPAPRIGHIQLM
                     DVETSAKALTLWGKEAPK"
     gene            complement(2644..4218)
                     /locus_tag="PK576_09290"
     CDS             complement(2644..4218)
                     /locus_tag="PK576_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOE61823.1"
                     /translation="MLNAANRTVIRLWKDESGVVFALTVIVFLTLFMMGLGVYAIGET
                     VRQRVELQNAADAAAYSAAITQADGLSRVAAINRAMSWTYVQMVRKEMDYIVDKWLER
                     VLLKWEIDNNLMRAFNAPSPCNNGVPWYVTGYNVSIGNGANHEKVLLNKRHLVTIDQI
                     KQARQNASRDGKDYPTLGGQIDDHKQTIRDMNKKEQKLLEDLPKRIEKTVREVLRANI
                     KDTWNDGFAEGARISFVIEQEKNPAQNYAEILKQDRENDFLRHSNYIPEDGKTAEKVF
                     GEGSEPENWFVETGQKEGIQRAYKQRNRVLLAEWHYTSSLWVPTKSGCRRVATYPRQL
                     REVKAEEVRDSRFTSEIAKANILRKNFFSKKGAIAVGVTRRLNNPLQFLVAGGELGII
                     RPFTVDNGTRHMWTVATARAGYNPPGKGKTQPPADCNGEYEETWEERVDRDDLWNLKT
                     SDWDATLLPLYRAWTQGENRNWDGTSGGKILAQIKEGSWQPLYGEGGSPENERAPKLM
                     GGGNVSYSETDAWVVH"
     gene            complement(4221..4787)
                     /locus_tag="PK576_09295"
     CDS             complement(4221..4787)
                     /locus_tag="PK576_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOE61824.1"
                     /translation="MLEFVMAAPVILSLILACMQFAHIWMAKQVVHYAAYCAARATLV
                     CHKDERDRAAEQAARQVCAWIVRGETAGEPAKRIPGWGEIPGSGGARRKTRLKGGQVE
                     DIGRWNVKATVEHDFALIFPLVGPMIGWALNPWGQNDAWQEQRADDTGNIGDADAVRY
                     PHIRFTETVVLPKPYVTVSKMGLPAPGW"
     gene            complement(4901..5428)
                     /locus_tag="PK576_09300"
     CDS             complement(4901..5428)
                     /locus_tag="PK576_09300"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOE61825.1"
                     /translation="MMETVCPLLCMAGIAAWAGFLSWSDCRTRRLPNALTLGAAVVTL
                     AFRWAYGGWDCLLDGFAAGAAAGGFMLVPFLLRGAGGGDVKMLFAAGAMVGWSRLLTL
                     LWFTSLAGVILGLVMLALGQVDGARLRHLLRCAVDWRYDRKAGAAGLPPREAAQVRVP
                     FSVAISAGLLVTMAG"
     gene            complement(5519..>5900)
                     /locus_tag="PK576_09305"
     CDS             complement(5519..>5900)
                     /locus_tag="PK576_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61826.1"
                     /translation="KRAPASQRPALRWPLADGRTNRRVNPSKERDMKKWNRNRTKRIL
                     CRLMGDRAGGVMMEYVILAVLIAAAVVVAVAMFGKTLVGMFDTAAKGATADHTGARQA
                     QDATRSEQSQKAQEAAQYHDSMHQ"
BASE COUNT         1068 a         1858 c         1719 g         1255 t
ORIGIN      
        1 catccgtaca gtatacccct ccttgggccg gtgccgcaag cgtcgccgtg ccgatcgtgc
       61 ctgcgttata tgctccagcg gcggtattgt ttgtcgcgtt tgtaattcgt ttcgtcccct
      121 gccgctgcca gcatctgctg tttcgtctcc ctgtcggcgc ggaacgatgc gcgtatggcg
      181 gtaggcggga ggagcccggg ttgcggcggt cccgtgaaaa cggagagaaa cggaaccaac
      241 atcggaagcg ccttccgata gaggaacgac cggttttttg tgcaaagagt gttcatcaag
      301 aacgctgcct tcctttcctc ttccttcgcg cattacttat gcatggagtc atgatactct
      361 ttggcgatat ccgcatcctc gcggcgatcc ccacgacgca tgttgaggtt ctcccgggca
      421 tcctgctcgc gcagcgtggt ggcctcgccc gcggcgatga acatgctggc gacggagcgg
      481 ctgaacacca ctaccgcgac caccacggcg gcggcgatca acacggcaag aatgacgtac
      541 tccatgttga cgccgcctgc gcgccgccgg ttatgcctga caagatgtgg cgtgttttta
      601 ttcacggatt ttctccacct accggaaggg tttcagtttg ggcggtgcct gcgaggacga
      661 atcggcaccg ccgggcgtac cgctcgaact ttccgggagt ggcgagggca acttcggttt
      721 cttctctggg ccatgcttct tgtcgaaggc ccaatccgcc agatccttga tactgtcctt
      781 gaaaaaggtg tcggtgaccc atccggcacc cgtttcgacc gtttttccgc cgacccaact
      841 gcctaccgcc ccaccgatga tcgcgccggg aacagcgccc acgccaccga accagactcc
      901 gatcgcggca ccggccttgg ccccaagcgc gccgccagcc caagagcctg tgaactcacc
      961 ccccattttc agcccctcct tcacggcgtt gtgaagggct ttggtgaaca gcttgcggtc
     1021 accgctattg taggtttcga tcacatggcc ggtgaaatgg gcgactttga ggcttttgag
     1081 gacgatcgtg atgactttga tcttgtcgcc cgcgcccttg agcgtgtctt tgtgcttatc
     1141 cagcaactcg ccgatgtctt tgccctctgc aagggctttt gtgatcgcct cacccaagtc
     1201 cttggtaatg ctcatgcccg cgtctgcggg aaggtagtag ggggaatctt ggaccgcatc
     1261 gagtccgtcg aggactttgt ccgctccttt caacccgagt ttcccggctt cggcgagaac
     1321 atcggagtgc tgcatgagac tggggcccaa actcacgaac ggggcgtctg ccatcttgat
     1381 cttgccgctg gggaaaccgc ccgcagcttg cggtgtggcc gccgcgacag aggcggctcc
     1441 ggtcgggagc aagccgaccg ccagaatggc gaaaacgaat cgttggatca gatgcgcaag
     1501 ggatgttttc atttcggcgc ctccttcccc cagagagtca gcgccttcgc gctggtttca
     1561 acatccatca attgaatgtg tccgatacgc ggggccggtt ccgcgcctag gaattcggcc
     1621 tcgatgaacc agcgcggtgc cgtcggattc accagtccca tgatcacgct gtccctggct
     1681 tttgaaaagt agacgggcgt gaaggttttc cgggtttccg gaggcagcgc gcacaccgtt
     1741 tcaatgatcg cgggcgattg cttttcggaa aggaatttgc ggagcgctgc gggatcgccc
     1801 gcagtcagtg cgcggatcag cacgccgctc tgaatcatcc atcccttgtt ttcggcggac
     1861 aggtaatcct gaaacatgtt cagacgcagc accaaacgcg ctttgatcag gatcagctcg
     1921 ccgaccggtg agtcaatgcg gtcccgcaac gccgcgggca ggagttccgg cgctccctcc
     1981 ggcggataga gccgggtgaa catggcaacc gacggcgcat aaagacggga aatcgccacg
     2041 gtgagcggtt cgcgcaacgg atgcagcgaa aacgatcctt ccggtgtgat tgtggcggag
     2101 tcgccgcgca ggctctcgcc gctgacaaaa cggaaatccg agaaactggc cttgggggag
     2161 gggcctgcct caacccgctg ctcgataacc agaagagcgt cggaccaggg actgtacagg
     2221 gccacgatgc catgcggtcc cttcatgccg cccacccaaa ccacggcgct ggtgaagaaa
     2281 tctttgagcg cgatggtttc atgttcgtta aagacctcgc gcacatcgcg ggtccacttg
     2341 gcctgaaagg cgccggtgcc ccattttttc acggcgccgt ccacatccga cgccgcatgc
     2401 agccggaaca ggttgaccgt gaggccatgc aggataccgg caccgacttg ttcggcggtt
     2461 ggctcgcttt cggaggtttt gcctccggtg ttgtcagctc cgctggcgga aggcatgaaa
     2521 accatgccga gtccggcgaa ggcggtccat gcggttaaga tcgtcatacg gagtctcctc
     2581 tcgaatgaag gtcgggttgg cctttgcatt gttctgtcct ttctttattg gacggtgtcc
     2641 ggttcagtga accacccaag cgtcggtctc gctgtacgaa acatttccgc cgcccatcaa
     2701 cttgggggcg cgctcgtttt cgggcgagcc gccctcaccg taaagtggtt gccaggaacc
     2761 ctctttgatt tgggcgagga tcttaccgcc ggaagtgcca tcccagttcc ggttctcccc
     2821 ttgcgtccaa gcgcggtaga gcggcagcag cgtggcgtcc cagtcgctgg tcttaaggtt
     2881 ccaaaggtca tcgcggtcga cgcgctcttc ccaggtctcc tcatattcgc cgttacaatc
     2941 cgcagggggt tgggtctttc ctttgccggg gggattgtat ccggcgcgcg cggtcgccac
     3001 ggtccacatg tggcgggtgc cgttgtcgac ggtaaagggg cggatgatgc caagttcgcc
     3061 gcccgccaca aggaactgaa gcggattgtt caggcgtcgg gtcaccccca ccgcaatcgc
     3121 tccctttttc gaaaagaaat tcttacgcag gatgttggct tttgcgattt ccgaggtgaa
     3181 gcggctgtcc cgcacctctt cggctttgac ctcgcgtaac tgtctgggat aggtggcgac
     3241 ccggcggcac cccgacttgg tcggaaccca cagcgagctg gtgtagtgcc attccgcgag
     3301 cagtacgcga ttacgttgct tgtaagcgcg ctggatccct tccttctggc cggtctcgac
     3361 aaaccagttt tccggctccg agccttcgcc gaacaccttc tccgcagtct tcccgtcttc
     3421 gggaatgtaa ttcgagtggc gcagaaaatc attttcccgg tcctgcttga ggatctcggc
     3481 ataattttgg gcgggatttt tctcctgctc gatcacaaag ctgattcggg cgccttccgc
     3541 gaagccgtca ttccaggtgt ccttgatatt ggcccgcaac acttcgcgca cggttttctc
     3601 gattcgtttc ggcagatctt cgagtagctt ctgctccttc ttgttcatgt cacggatcgt
     3661 ctgtttatgg tcatcgatct ggccgcccaa ggtggggtag tctttgccgt cacggctggc
     3721 gttctggcgc gcctgtttga tctgatcaat ggtcaccagg tgccgtttgt tcaacaggac
     3781 cttctcatgg ttcgcgccgt tcccgatcga gacgttgtag ccggtgacat accaggggac
     3841 tccgttattg cagggactgg gcgcattgaa ggcgcgcatc aggttgttgt cgatctccca
     3901 tttcaggagg acgcgctcca gccatttgtc aacgatgtaa tccatttctt tacggaccat
     3961 ctgcacatag gtccaggaca tggcgcggtt gatagcggcc acgcggctga ggccgtcagc
     4021 ctgtgtgatc gcggccgagt aggctgcggc atcggcagcg ttctgaagtt ccacgcgctg
     4081 ccgcacggtt tcgccgatgg cgtagacacc cagacccatc atgaacagcg tcagaaagac
     4141 gatgaccgtg agggcaaaga ccactccgct ctcgtctttc cacaaccgga tgacggttct
     4201 gtttgccgcg ttcagcatca ttaccaccca ggggccggga ggcccatttt ggatacagtc
     4261 acatagggtt tgggcaagac gacggtttcg gtaaaccgga tatgggggta ccgcaccgca
     4321 tccgcatcgc cgatgttgcc tgtgtcgtca gcccgctgct cctgccatgc gtcattttgg
     4381 ccccacgggt tgagcgccca gccgatcatc gggccgacaa ggggaaagat caacgcgaaa
     4441 tcatgttcca ccgtggcttt gacgttccag cgtccgatgt cctcgacctg cccgcctttc
     4501 agtctggttt tgcgccttgc tccgcccgaa cccggaatct cgccccagcc cgggatgcgc
     4561 ttggccggtt cgccagccgt ttcgccgcga acgatccacg cgcagacctg gcgcgcggcc
     4621 tgctctgcgg cccggtcccg ctcgtctttg tggcagacca gcgtggcgcg ggcagcgcaa
     4681 taggcggcgt agtggaccac ctgcttggcc atccagatgt gggcgaactg catgcaggcc
     4741 agaatcaggg agaggatcac gggcgcagcc atgacaaact ccagcatcac cgatccgcag
     4801 gaacgggtcg atctgttctg tgggcaagcc tgcaaggggc tttgggtctc tccgctaaac
     4861 atttcagatc tccgtcttaa tacgcccggg atcacactct tcaccctgcc atcgtaacca
     4921 gcaagccggc agagatcgcc acactgaagg gcacgcggac ctgtgccgcc tctctgggag
     4981 gcaaacctgc ggcaccggcc ttacggtcat aacgccaatc taccgcgcat cgcaacaggt
     5041 gccgcaaccg tgcgccatcc acttgcccca gcgccaacat cactaagccg aggatcaccc
     5101 ccgccaggga ggtgaaccac aacaaggtca ggagcctgga ccagccgacc atggctccag
     5161 ccgcaaaaag cattttcacg tcaccaccgc ccgcgccccg caacaggaag ggaaccagca
     5221 taaagccgcc ggccgccgca ccggcggcga atccgtccaa caagcagtcc catccgccgt
     5281 aggcccagcg gaacgccaag gttacgacag ccgctcccaa agtgagggcg ttcggcaaac
     5341 gccgcgtacg gcagtctgac caagacagaa aacccgccca tgccgcaatt cccgccatgc
     5401 acagtaatgg gcagaccgtc tccatcatta cgcttaccct ccgctaccgc catccggagg
     5461 cggaaaagcc tccggcggcg gagcggttct ttgaataacg ggtcggactt ccgacgcgtt
     5521 actgatgcat gctgtcgtga tactgggccg cctcttgggc cttctgactc tgctcggacc
     5581 gcgtcgcgtc ctgagcctgc ctggcgccgg tgtggtccgc ggtcgcaccc ttggccgccg
     5641 tgtcgaacat gccgaccagg gtcttgccga acatcgccac agcgaccacc acggccgccg
     5701 cgatcagcac cgcgaggatc acatactcca tcatcacgcc acccgcccgg tcgcccatca
     5761 gtcggcacag gatgcgtttg gtccgatttc gattccactt tttcatgtcc cgctcctttg
     5821 acgggttgac ccgtcggttt gtccgcccgt cagccagcgg ccagcgcaac gctggccgct
     5881 ggctggcggg agcgcgcttc
//
LOCUS       DAOSLE010000159         5891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12218_length_5891_cov_6.146162, whole genome shotgun sequence.
ACCESSION   DAOSLE010000159 DAOSLE010000000
VERSION     DAOSLE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12218_length_5891_cov_6.146162"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..260)
                     /locus_tag="PK576_09310"
     CDS             complement(48..260)
                     /locus_tag="PK576_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61827.1"
                     /translation="MKRAAYAMIASAATLTASAATLDAPRLPVPPFADREVSAAHALP
                     AGRADGPRRVPDRRARQHLIYSGQNS"
     gene            649..1065
                     /locus_tag="PK576_09315"
     CDS             649..1065
                     /locus_tag="PK576_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOE61828.1"
                     /translation="MKGKTQTVSPERRAQLQAELRDAVATRQTGYREKALRILPHVCA
                     SCGRSFEGKRLKELTVHHKDHNHRNNPPDGSNWELLCIYCHDHEHEKTLLGGYYEDAP
                     ARKDVLKPSIFSPFSVLDDLSPVTPDTDAATPDATP"
     gene            1297..4203
                     /locus_tag="PK576_09320"
     CDS             1297..4203
                     /locus_tag="PK576_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61829.1"
                     /translation="MRGAGRRIRGVGWLGLAFMAGSLGAQSIIDGVLQGVPPPPAQPG
                     DETWTFVNDLKQPLWAENHAWPQRAAEAGEADLSRGARVIQGFPDPEGVLETAVDDLR
                     AFLAAGRVLPDGPYVIETGRIETPVFESYRIEVEPNRCRILAADTEGVRRGIVYVEDR
                     MLAAEGPFLPLGSVTRKPFIASRISRCFFGPIKRPPRYRDELMDDVDYYPEAYLNRLA
                     HEGINGLWLTVTFRDLCRTEITPGFGEQADRRFAKLRRTVERCRRYGIRIYLFAIEPA
                     AWDADDPVLKNHPELGGAKAGGKLMFCPSSETAQRYLYEAAKGIFSAVPHLGGLINIT
                     HGERYTSCQSVVVATSNGRTTCPVCAKKEPWEIFQATLEPLSRGMREANPHAELIAWF
                     YMPQDADPGKWVYAIPQHGLSNVVYQFNFESGVTKTDFGRVRLGGDYWISTPGPSARF
                     ERLADLARESGVAFSAKIQTGCSHEVATVPFVPVPGLLYRKFRAMRELGVSRVMLCWY
                     FGNYPGIMNKAAGELAFEPFPETEQAFVSELARAEWGRARAGTVAQAWERFAEGYGHY
                     PLVTPFQYWGPMHDGVVWPLLLLPQDAPLAPTWLIAANRGERVGDPFPPSGDRIAEAL
                     GGAYTLQEAIEACRAMSVMWDQGVGLLRTLERDSGLPKARRQDIGVSRALGLQFRSGL
                     NILRFYDLRERLARETVKARQLALLSEMRGIVEKEAAGGVELKELCRADSRIGFHSEA
                     EGYKYFPAKIAWRGEQLRRVLDTEMPALERSFAEGKDVFAAYAGRQPEGPCARSVFRA
                     DIARQIEEGSAWLKEQDWQMDSTTNTFRWTCRHDRDALYLLFTCDNTPSAKRADGTVS
                     LILEPRRLWPSMTCRVSADGKAHSSPDVMGAHYAFQSGVRREEGGWSGWMRIPFVSIG
                     IPSERPAPVRINVVRVAAGSRDEQAWITRRPWPYRLRVSGGNPADLGWLLFDGP"
     gene            4243..5052
                     /locus_tag="PK576_09325"
     CDS             4243..5052
                     /locus_tag="PK576_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOE61830.1"
                     /translation="MLATFHNHSVWSDGADTLEALYEQAGRLGVSLLGMSEHFCAYPD
                     GAAVAGYLPADRIDAYLSTLREVGQTGPVEVVAGLEFDWTEAGAEWVRPYAEGLPLDY
                     RIGSVHYVAGEGIDIDASFWMSKTQEEIDAVYAGYWRLIRGMAESGLFDIVGHLDLPK
                     KFGFVSGPSLRGLEDEALDAIAASGMTVEFNTAGFDKPCAEGYPSEDLLKRCRQRGIP
                     VTLSSDAHRAAHVIRGFERGAAVLAAAGYDRVARFRGREQWFEGLDDAVVR"
     gene            5152..>5891
                     /locus_tag="PK576_09330"
     CDS             5152..>5891
                     /locus_tag="PK576_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE61831.1"
                     /translation="MFGTGMISISLPLASLIGGFMESNLMGKGIVLVQLASSIVAAAI
                     IIGKNRELSSVMRNSKRFMREFLLGHDVLDYYLLRKKGSFSALELIYERTCERMVKLL
                     DPDMRRSVIGRQRDAAGAALSAREIELVRSTCEHTVQDEELRIEYGMGLLATIVTASP
                     MLGLLGTVWGVLDAFADMGSKGTVLLSVIAPSISSALVTTVVGLLVAIPSAICYNNLA
                     AKVRQITTDMVGFADELMGRIACEFQGRT"
BASE COUNT         1054 a         1611 c         2059 g         1167 t
ORIGIN      
        1 tttcaccccc gacagtcaca cgcgtattct caaccctgtt gcgcccgtca agagttctgt
       61 ccagagtaaa taaggtgttg ccgagcgcgt cggtccggga cacggcgcgg cccgtccgcc
      121 cggcctgcgg gcagggcgtg cgccgccgac acctcgcggt cggcgaaagg cggcaccggc
      181 aggcgcggcg cgtccagcgt ggcggcggat gccgtcagcg ttgccgccga ggcgatcatt
      241 gcgtatgcgg cgcgtttcat ttttcgtttc cttccctggt tttccggcag ggaaaaggcg
      301 cggaccccgt tccgtgcctt atctgaggtg ccaccaagca cccgccagga agcccggcaa
      361 cgaggccgcg cccgatcggg cgcgtccccg tgcatcccgc tcctcccaaa gatttgacgg
      421 cttttcgtag ggaacgtttt gcgcgaaaac gcaaaaatca gtttttaggt tccggtctct
      481 tccgcatgcg ttgtccttgc cgattcaatc aatccagcca accatcagaa tgaacatgct
      541 cctttttgcg gggactttgc acccgttttt tagggtaagg gtttctgccg tcgacagtgc
      601 gattgaaacg cattgacaag aacggcacgg gacgcctatc gtggaggcat gaaaggaaaa
      661 acacaaaccg tttcaccgga acgccgtgct cagctgcagg ccgaactccg cgatgccgtt
      721 gcgacgcgcc agacgggtta ccgcgaaaag gcgttgcgta tcctgcctca cgtttgcgcg
      781 agttgcggac gtagttttga aggaaagcgg ctcaaggaac tgacggttca tcacaaggat
      841 cacaaccatc gcaacaatcc gccggacggc agcaactggg agttgctttg catctattgt
      901 cacgaccatg agcatgagaa gaccctgctc ggcggttact acgaagacgc gcccgcccgg
      961 aaggatgttc tcaaaccctc gatcttctcg cctttttccg tgctcgacga cctgtcgccc
     1021 gtcacgccgg acacggatgc cgccacgccg gatgcgacgc cctgaacccc gtgcgcgcat
     1081 gtcgggccgt aacagaaaaa agggttgttt ttgacgggtt cgaggcttgt tggattcggg
     1141 ttcctgtgcg aatatgactg cggtggattg tgcggtccga gttggatgtg accaaacggt
     1201 tgccgccaga ttctggagat gctcggggag cgcgctcgaa aggtggaacg ggaggacgag
     1261 atgggatacg ccgcaaggtg gaaagggggg gacgggatgc gtggtgcggg aagacggata
     1321 cggggcgtcg gatggttagg gctcgcgttt atggcgggca gtctgggggc gcagtccata
     1381 atcgatggtg tgctgcaggg tgttcccccg cctcccgcgc agcccggcga tgagacctgg
     1441 acttttgtca acgatctgaa gcagccgtta tgggcagaga accacgcttg gccgcagcgc
     1501 gcggcggagg cgggggaggc ggatctgtcg cgcggggcgc gcgtcataca gggattcccg
     1561 gatcctgaag gggtgctcga aacagcggtt gacgatctgc gcgcgtttct ggctgcgggc
     1621 cgtgttctgc cggacgggcc ttacgtgatc gagaccggcc ggatcgagac accggtgttt
     1681 gagtcttatc ggatcgaggt ggaaccgaat cgttgcagga ttcttgccgc cgacaccgag
     1741 ggggttcggc gcggaatcgt ttatgtggag gatcgtatgc ttgcggccga agggccgttt
     1801 ctgccgctgg gttcggtcac gcgcaaaccg ttcatcgctt cccgtatctc ccgttgcttt
     1861 ttcgggccga tcaaacgtcc gccacgatac cgcgacgagt tgatggatga tgtggattat
     1921 tatcccgaag cgtacctcaa ccggctggcg cacgagggca tcaacggcct ctggctgact
     1981 gtgacgttcc gcgacctgtg ccgcacggag atcacgcccg gattcggcga gcaggctgat
     2041 cggcggttcg ccaaactgcg gcgcacggtc gaacggtgcc gtcggtacgg cattcgcatc
     2101 taccttttcg cgattgagcc tgcggcgtgg gatgcggatg acccggtact caaaaaccac
     2161 ccggaactgg gcggcgcgaa ggcaggcggc aaactcatgt tctgcccgag ttcggagacc
     2221 gcgcaacgtt atctgtatga ggccgcgaag gggatctttt cggcggtccc gcacttgggc
     2281 ggtctgatca acatcacgca cggcgaacgg tacaccagtt gccagagcgt ggtcgtggca
     2341 accagtaacg gccgcacgac ctgccccgtc tgtgcgaaaa aagagccgtg ggaaattttc
     2401 caggcgacgc tggaacccct ctcacggggt atgcgcgagg ccaatccgca tgctgagctg
     2461 atcgcgtggt tttacatgcc gcaggacgcg gatccaggga agtgggtgta cgcgatcccg
     2521 cagcatggtc tttcgaacgt tgtctaccag ttcaacttcg agtccggggt cacgaaaacg
     2581 gatttcggac gagtccggct gggcggggat tactggatct ccacgccggg accgagtgcg
     2641 cggtttgagc ggctggcgga tttggcacgg gagtcaggcg tggccttttc ggccaagatc
     2701 cagaccgggt gctcgcatga ggtggcgacc gtgccgtttg tgccggtacc gggattgctg
     2761 taccgcaaat tccgggcgat gcgcgagttg ggggtgagcc gggtgatgct ctgctggtat
     2821 ttcggcaact atccggggat catgaacaaa gccgcgggtg aactcgcttt cgaaccgttc
     2881 cccgaaacgg aacaggcttt tgtatcggag ctggcgcgcg ccgaatgggg ccgcgcgcga
     2941 gccggaacgg tggcacaagc ctgggagcgg ttcgcggagg ggtatgggca ctatccgctg
     3001 gtcacaccgt ttcagtactg gggacccatg catgacgggg tggtctggcc gttgctgctg
     3061 ctgccgcagg acgcgccgtt ggcaccgacc tggctgatcg ccgccaaccg gggggagcgg
     3121 gttggggatc ccttcccgcc cagcggagac cgcatcgcgg aggctttggg cggggcttac
     3181 acattgcagg aagcgatcga ggcgtgtcgc gcgatgagcg tcatgtggga tcagggcgtg
     3241 gggctgttgc ggacgctgga gcgcgattcc ggcctgccga aggcgcggcg gcaggatatc
     3301 ggcgtgtcgc gcgcgctggg gttgcagttc cgcagcggtc tgaacatctt gcggttctat
     3361 gatctgcgtg agcggctggc gcgggaaacc gtgaaggcgc ggcagctcgc cctgctcagc
     3421 gagatgcgcg gaatcgttga aaaggaagcg gccggaggtg tcgaactgaa ggagttgtgc
     3481 cgggccgact cgcgtatcgg cttccactcc gaagcggagg ggtacaagta ctttccggcc
     3541 aagatcgcgt ggcgcgggga gcagttgcgg cgcgtgctcg ataccgaaat gccggctctg
     3601 gagcgttcgt tcgccgaggg aaaggatgtg ttcgccgcgt atgcgggcag gcagcccgag
     3661 gggccttgcg cgcgtagtgt gtttcgcgcc gacattgcgc ggcaaatcga agagggttcc
     3721 gcgtggttga aggagcagga ttggcagatg gattccacga cgaacacctt ccgctggacc
     3781 tgccgccacg atcgcgatgc gctctatctg cttttcacat gcgacaacac cccttccgcg
     3841 aagcgtgctg acggaacggt gagcctgatc ctcgaaccgc gtcgcctctg gccgagtatg
     3901 acctgccggg tatccgccga cgggaaggcg cactcctctc ccgatgtcat gggggcgcac
     3961 tacgcgttcc agtccggggt tcgacgtgaa gagggcggct ggagcggatg gatgcggatt
     4021 cctttcgttt cgatcggcat cccgtcggaa agacctgcgc cggtgcggat caatgtggtg
     4081 cgcgtcgcgg ctggcagccg cgacgaacag gcctggatca cgcggcgtcc ctggccctat
     4141 cggctgaggg tgagcggagg caatccggcg gatctcgggt ggctgctctt tgacggcccc
     4201 tgatcacaca aactgacggc agcgtaagaa aaggatgcag acgtgttggc aacatttcat
     4261 aaccattccg tgtggagcga cggggcggac accttggagg cactttacga acaggccgga
     4321 cgacttggcg tgtccctgct gggcatgtcc gaacattttt gtgcctatcc ggatggcgcg
     4381 gcggttgccg gatacctgcc ggccgaccgg atcgacgcgt atctgagcac gctgcgcgaa
     4441 gtcggacaga ccggaccggt ggaggttgtc gcgggactgg agttcgactg gacggaggcg
     4501 ggagcagagt gggtccggcc gtatgcggag gggctgccgc tggattaccg catcgggtcc
     4561 gtgcattacg tcgcggggga agggatcgac atcgacgcat ccttctggat gagcaagacg
     4621 caagaggaga tcgacgcggt gtatgccggg tattggcgtc tgatccgcgg gatggcggag
     4681 agcggtctgt tcgatatcgt cgggcatctg gacctgccca agaaattcgg ttttgtatcc
     4741 ggtccgagtc tgcgcggttt ggaagacgag gcgttggacg cgattgccgc gtccggaatg
     4801 acagtggagt tcaatacggc ggggttcgac aagccgtgcg cggaagggta tccgtcggaa
     4861 gatctgctga aacgctgtcg gcagcgcggc ataccggtca cgctctcgtc ggacgcgcac
     4921 cgggccgcgc atgtgatccg gggttttgaa cggggcgccg cggttctggc tgcggcgggg
     4981 tatgatcgtg tcgcccggtt ccgcgggcgc gaacagtggt tcgagggtct ggacgatgcg
     5041 gtggtccgtt gagggggtgc gaatatccgg cataacggat ggttgacatc cgggcggggg
     5101 tgacgcatgc taagtcttaa ctgctttcag tgttttcagg aggcgacacg catgttcggg
     5161 acaggaatga tatcaatcag cttaccgctg gcttcgttga tcggcgggtt catggagtcc
     5221 aatctgatgg gcaaggggat cgtgctggtc cagcttgcct cttcgattgt ggcggcggcg
     5281 atcatcatcg ggaaaaaccg ggagctgagc agtgtgatgc gcaacagcaa gcgcttcatg
     5341 cgcgagtttc tgctgggaca cgatgtgctg gattattacc tgctgcgaaa aaaaggttcg
     5401 ttctcggcgc tggaactgat ctatgagcgc acatgtgaac ggatggtgaa gctgttggac
     5461 cctgacatgc gccgttcagt gatcggacgg cagcgtgacg cggctggcgc ggccctttcg
     5521 gcgcgcgaga tcgaactggt gcgcagcacc tgcgaacata ccgtgcagga tgaggagttg
     5581 cggatcgagt acgggatggg gctgttggcg acgattgtga cggcttcgcc gatgctgggg
     5641 ctgctcggca cggtgtgggg cgtactggat gcgttcgcgg acatggggtc caaggggacg
     5701 gtgctgctgt ccgtgatcgc gccgtcgatc tcctcggcac tggtcaccac ggtggtgggt
     5761 ctgctggtcg cgatcccgag tgcgatttgc tacaacaatc tggcagcgaa ggtgcggcaa
     5821 atcacgaccg atatggtcgg gttcgcggat gaactgatgg gccgcatcgc ctgcgagttc
     5881 caagggagga c
//
LOCUS       DAOSLE010000160         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12641_length_5740_cov_9.252595, whole genome shotgun sequence.
ACCESSION   DAOSLE010000160 DAOSLE010000000
VERSION     DAOSLE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12641_length_5740_cov_9.252595"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PK576_09335"
     CDS             complement(<1..368)
                     /locus_tag="PK576_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE61832.1"
                     /translation="MNRVTGLGAITAKRMAERFGSLAAAFEASESDLRTVAGIGLDKA
                     QQFWSDLRQARADDELARAAKKGVKLVTWDDPGYPALLKQIADPPLVLYVAGAVEALD
                     RPTVAIIGTRRPTIYGRDCAR"
     gene            578..1330
                     /locus_tag="PK576_09340"
                     /pseudo
     CDS             578..1330
                     /locus_tag="PK576_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(1340..2098)
                     /locus_tag="PK576_09345"
     CDS             complement(1340..2098)
                     /locus_tag="PK576_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61833.1"
                     /translation="MVLVSEMNGRRRDITLAVLMAVVATTATGVLAEREEELLDVLDF
                     DITAAWDSRYVGEGRDNLGGDSLASTTIEAAYESLSVNVWYATSPDTDYRELNIKPGY
                     TFEWNDWEACVFYKFRRFLSDKESVNEAGADLSYSGLPAGLAAGVEGCYSFDAEGVFF
                     VTYINRACEVADWLTLTPAALMGWNANYAADGHDGANHLALVLEAAVPLKAGLELSGN
                     IAYSFAINDNADKYADDEGLRSMFYGGVALRASF"
     gene            complement(2140..3246)
                     /locus_tag="PK576_09350"
     CDS             complement(2140..3246)
                     /locus_tag="PK576_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOE61834.1"
                     /translation="MAIDLNRWLEELAAQGGSDLHLAADNPPVIRLHGRLERLGERDA
                     PLSAEAITEAIAGVVDAEAMARLRKTGELDSAFELKDGTRLRINAYRQQGDFAAAIRL
                     LPNRFFELEELGLDVGLLRRICAMQSGLVLVTGATSSGKSTTIASIVNEINKTQALHI
                     HTIEDPVEYRHVSQQSFVTQREVGRDTESFAEALRRSLRQDPDVIVVGEMRDLETMTA
                     ALTLAETGHLTFATLHTSDAVQSVSRIISAYPAMHQAQVRVQLASTLRVVMSQKLVPW
                     ANGKGRSLAAEILVATPSVCAMIREEKTHQMRTAMQTGGEHGMQTLNQSLLSLLNRRI
                     IDRATALEYSEDKALLLEELEHHTGQTTKRKGIR"
     gene            complement(3236..5191)
                     /locus_tag="PK576_09355"
     CDS             complement(3236..5191)
                     /locus_tag="PK576_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61835.1"
                     /translation="MSRYRVMAVGHKLAGALQSRGCIWILSMAATLSGPLASLTVAQA
                     QSEPVAIESVFVRDMALRDFAELMTRGCESEWKVLVSEQAGDKRISFYLSDTGIEETL
                     RSICATHGLWYRQSPRSDIVQIITMEEYQKGLNLYADETVEVVPLMYPMPEEIGDALA
                     RLFQDRVVWDPPPEDIADDMFNIESALDRMETISDRATLVDTENMDGMGTGTGRDGSR
                     GYGSSYSRRGDSWGRSGRYGRSYGSDRYGRSSGSGRYGSGSGRSGEEQTVEEVVKQQR
                     LAVEAQQAARGIPEEMGGRNDRPGLVYIAAARSANALVLRSSDAASIETIKSVIHDLD
                     KPKPQILLEVKVLDIQFGDEDARGVDWLFQRELGSKDFMLSGGRATGISTEPGSLISS
                     SDPLTLVPQGTGLDPMATVFSLVSDDVRARLQFLEDKRRIRALATPSLLVADNEASRI
                     FIGSEVTVLEKVEPETSFIGTERPQPVTTYTVTSPRKRIGTTLLITPKIHADRTVTIR
                     LLQEETQLGPQRTVEYGQTATDQFAAQDIEERSVTTTVLAKDGHVVAIGGLIRDRHEE
                     RETGIPVLMHIPILGHLFKRTILDETRGELLVLIRPRVLLAPGEGQAASRETIERVNR
                     RVEEFERKWKKFDAQAEAPAAEAPDGN"
     gene            complement(5188..>5740)
                     /locus_tag="PK576_09360"
     CDS             complement(5188..>5740)
                     /locus_tag="PK576_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61836.1"
                     /translation="GTPALPTAHTQNTRSARMLPVTNRLRVAVLVALFGLSRMALATD
                     AAVATNTPAEPLQEAEIATADAVALPAEPKIPVGDPFAMRGLAEGDGFVSAGPAAIPP
                     GIRVVAILAVAGKSPVGALEIPGSTVLHFVREGDVVQVDRAAGSEAKDAVGSQLYLLV
                     KSITKSQIEIAPRTRPHDVRIYR"
BASE COUNT         1018 a         1934 c         1677 g         1111 t
ORIGIN      
        1 cgcgcgcaat cacgcccgta gatcgtcggg cggcgcgtac cgataatcgc caccgtcggg
       61 cggtccaacg cctccacggc gcccgcgaca tacagaacca gcggcgggtc ggcgatctgc
      121 ttgagcaagg ccgggtaacc ggggtcgtcc caggtgacca gcttcacgcc cttcttggcc
      181 gcgcgcgcca gctcatcgtc ggcacgcgcc tgccgtaggt cggaccagaa ctgctgcgcc
      241 ttgtccaggc cgatgcccgc cacggtccgc aggtcgctct ccgaggcctc gaaggccgca
      301 gccagcgatc cgaaccgctc cgccatccgc ttggccgtga tggcgcccag gccggtcacc
      361 cggttcatag cgattaaagc gtcccgctct gtcacattat cctctcaaag tcctacaccg
      421 tccatccggt tcacaaaatg aggtagggta attagcatat tcgcgcgtat ctgacaagca
      481 agacgcgaaa atcccccttg cgtcaaatcc tgaaacctca tactcttcag gtatctgcat
      541 ctccgcaatt gagcatcaag cgttttgatt tcacactatg gctactgatt cttaccaacc
      601 gccctacagc ctcacgccga ccatgctccg cctggttgag gagatcggcg gagcactggg
      661 ccgactgcgg gccggtcacg gttccatcgc tccccgcctg cggcgcggta accgtatccg
      721 caccgttcag gcctcctgcg ccatcgaagg caacaccctc agtgtcgaac aggtcacggc
      781 ggtgctcgaa ggcaagcgcg ttctgggcgc gccccgcgaa atccacgaag tccgcaacgc
      841 ctttgccgcc tacgagcgca tgttcgagtg gtcgccccat tcacgcgacg acctgttggc
      901 cgcacacggc gtcctcatgg ccggtctgat cgacgagccg gggcgtttcc gctccggcgg
      961 tgtcggtatc cagcgcggcg cggcaatcat ccacctcgcg cctcccgccg accgcgtgcc
     1021 cgccctgatc gccggtcttc tggcctggct cagagacacg ccggaacacc cgctcatcgc
     1081 cagttgcgtc ttccactacg agttcgaatt catccatcct ttccaggacg gcaacggccg
     1141 catggggcgc ctctggcaga cccttatcct cagccgctgg aaccccctct tcgccgctgt
     1201 ctccgtcgaa accctcacca gccagcatca ggccgactac tacgccgcac tgaatgccag
     1261 caacaaggcg gcaacctgca ccccgttcat cgagttcatg ctcgccatga tccgcgacgc
     1321 cctgcgggaa ctgctcgtgc tagaatgaag cgcgtaaagc aactcctccg tagaacatgc
     1381 tgcgtagccc ctcatcgtca gcgtatttgt cggcattatc attgatcgca aacgaatagg
     1441 cgatattccc tgacagttcg agccccgcct tgagcggcac ggccgcctct agcaccagcg
     1501 caaggtggtt cgcaccgtcg tggccgtcgg ccgcgtagtt tgcgttccac cccatcaacg
     1561 cggcgggtgt gagggtcaac caatccgcga cctcgcacgc cctgttgatg tatgtcacga
     1621 agaacacgcc ttcggcatcg aaggagtagc agccttcaac tccggcggcg agcccagcgg
     1681 gcagtcccga gtacgagaga tctgcgccag cttcgttgac actctctttg tcgctcaaaa
     1741 aacgccggaa cttgtagaag acgcaagcct cccaatcgtt ccattcaaag gtatagcccg
     1801 gcttgatgtt caactcccgg tagtccgtgt ctgggctggt cgcataccac acattgaccg
     1861 agaggctttc gtaagcggcc tcaatcgttg tactcgccag actgtcgccg ccaaggttgt
     1921 cgcgcccctc gccaacatag cggctgtccc atgcggcagt aatgtcgaag tcgagaacat
     1981 ccaagagttc ctcttctctc tcggcaagca cacccgttgc ggtcgtcgca acaaccgcca
     2041 taagaaccgc cagtgttatg tcccgtctac gtccattcat ttcacttact agcaccacgt
     2101 cgttttcctt tttagttgtt tctcttcttt ggcatcgttt catcgtatgc cttttcgttt
     2161 cgtcgtttga ccggtgtgat gttcgagttc ctcaagcaag agcgccttgt cttcgctgta
     2221 ttccagcgcg gtggcgcgat caatgatgcg gcggttcagg agcgagagca gggactggtt
     2281 gagggtctgc atgccatgct cccccccagt ctgcatcgct gtgcgcatct ggtgggtctt
     2341 ctcttcgcgg atcatggcgc acacgctcgg ggtggcgacc agaatctcgg ccgccagcga
     2401 acggcctttg ccgttggccc acggcacgag tttctggctc atgaccacgc gcagcgtcga
     2461 ggcaagctgc acgcgaacct gcgcctggtg catggcggga tacgcgctga tgatgcgcga
     2521 gaccgactgc acggcgtcgg acgtgtgcag cgtcgcaaac gtcaggtgcc cggtctcggc
     2581 cagcgtcaag gcggcggtca tggtttcaag atcgcgcatt tcgcccacga caatcacatc
     2641 ggggtcctga cgcaggctgc gccgcaaggc ctcggcaaag ctctcggtgt cgcgcccgac
     2701 ttcacgctgc gtcacaaagg attgctggct gacgtgccgg tactcgaccg gatcctcgat
     2761 ggtgtggata tggagggcct gggtcttgtt gatctcattc acgatactgg cgatggtggt
     2821 ggatttgccg ctgctggttg ccccggtgac gagcaccaga ccggactgca tggcgcagat
     2881 gcgccgcagg agccccacgt cgagacccag ctcttccagc tcgaagaagc ggttgggcag
     2941 caggcggatc gcggcggcga agtcgccctg ctgacggtag gcgttgatgc gcaggcgcgt
     3001 gccgtctttc agctcgaagg cagaatcaag ctcgccggtt ttccgcagac gcgccatcgc
     3061 ctccgcgtcg accacaccgg caatcgcttc ggtgatcgct tcggcgctca aaggtgcgtc
     3121 ccgctcgcca agcctttcga gacggccgtg cagacggatg accggcgggt tgtccgccgc
     3181 caggtgcaag tcgctgccgc cctgggcggc cagctcctca agccaacgat tcaggtcaat
     3241 tgccatcggg tgcctccgca gccggcgcct cggcctgggc gtcaaacttt ttccacttgc
     3301 gctcaaattc ctcgaccctc cgattaaccc gctcaatggt ttcacgggag gcggcctggc
     3361 cttcgcccgg cgccagcagg acgcggggcc ggatcagcac cagcaactcg ccgcgcgtct
     3421 cgtccagaat cgtgcgcttg aagaggtgcc ccaagatcgg aatgtgcatc aaaacgggga
     3481 tgccggtctc gcgctcctcg tgacgatcac ggatcaggcc gccgatcgcg acaacgtggc
     3541 cgtctttggc gaggactgtg gtggtgacgg agcgctcttc gatgtcctgc gcggcgaact
     3601 ggtcggtcgc cgtctgtccg tactcgacgg tccgctgcgg cccgagctgc gtctcctcct
     3661 gcaacagccg gatcgtgacg gtgcggtcgg catggatttt tggggtgatc agcaacgtgg
     3721 ttccgatccg cttgcgcggc gaggtgaccg tgtacgtcgt caccggctgg ggcctttccg
     3781 tgccgatgaa acttgtttcc ggctcgacct tctccagcac ggtcacctca ctgccgatga
     3841 agatgcggga ggcttcgttg tcggccacga gcaggctcgg cgtcgccaag gcccggatgc
     3901 ggcgcttgtc ctccagaaac tgcagccgcg cccggacgtc gtcgctgacg aggctgaaaa
     3961 ctgtggccat gggatccagc cccgtgcctt gcggcaccag tgtgagggga tcggacgagc
     4021 tgatcaggct gccgggctct gtcgaaatgc cggtggcgcg gccaccgctc agcataaagt
     4081 ctttcgagcc cagttcacgc tggaacaacc agtccacgcc gcgcgcatcc tcatccccga
     4141 actgaatgtc gagcaccttg acctcgagca ggatttgggg tttcggtttg tccagatcat
     4201 ggatgacact cttgatcgtt tcgatcgagg ccgcatcgct agaacgcagg accagagcgt
     4261 tggccgaccg cgcggcggcg atgtaaacca accccggccg gtcgttgcgc ccgcccatct
     4321 cctcggggat gccccgtgcc gcctgctgtg cctccacggc aagacgctgt tgtttgacca
     4381 cttcttcgac ggtctgctcc tcccccgacc tgccggagcc gctgccgtac cggcctgacc
     4441 ccgaactgcg gccgtaccga tccgacccat aactgcggcc gtaccgcccc gatcgacccc
     4501 acgaatcgcc ccggcggcta tacgaactgc cgtaccctcg ggagccgtcc cggcccgtgc
     4561 ccgtgcccat tccgtccatg ttctcggtat cgaccagcgt ggcacggtcg gagatggtct
     4621 ccatgcggtc gagggcgctc tcgatgttaa acatgtcgtc ggcgatatcc tcgggcggcg
     4681 gatcccagac cacccgatcc tggaacaatc gtgccagcgc atcgccgatc tcttccggca
     4741 tcggatacat gagcggaaca acctcgacgg tttcgtcggc gtacaggttc agacccttct
     4801 gatactcctc catcgtgatg atctgcacga tatccgagcg gggcgactgg cgataccaga
     4861 ggccgtgagt ggcacagatc gagcgtagcg tctcctcgat gccggtgtcg gagagataga
     4921 agctgatgcg cttgtcgccc gcctgctcgc tgaccagcac tttccattcc gactcgcatc
     4981 cgcgcgtcat cagctcggcg aagtcacgca acgccatgtc gcgcacaaac acggattcga
     5041 tcgcgaccgg ttcggattgc gcctgcgcca ccgtcaagga ggccaacggc cccgacaggg
     5101 tcgccgccat gctcagaatc cagatgcacc cgcgcgattg cagcgcaccc gccaacttat
     5161 ggccaacggc catcactcta taacgactca tctgtaaatc ctcacatcat gcgggcgcgt
     5221 gcgcggcgcg atttcaatct ggcttttcgt gatagacttc accagcaggt acaactgcga
     5281 gcccaccgca tccttggcct cgctccctgc agcgcgatcc acttggacca catccccctc
     5341 acgaacgaag tgcagaactg tggagcctgg aatctccaac gcgccgaccg gcgacttgcc
     5401 ggcaaccgcc agaatcgcga caacacgaat gcccggcggg atcgccgcag ggccggcgct
     5461 gacgaatccg tcgccctctg caaggcctcg catagcaaag ggatcgccga ccggaatctt
     5521 gggttccgcc ggcagggcaa ccgcgtcggc cgtcgcaatt tcggcctcct gaagcggttc
     5581 cgccggggtg ttggtcgcca cggcggcatc cgttgccaag gccatgcggg atagcccaaa
     5641 gagggcgacc agcacagcaa cccgcaatcg gttagtgaca gggagcatgc gagccgagcg
     5701 cgtgttttgt gtgtgcgcag tcgggagcgc gggcgtcccc
//
LOCUS       DAOSLE010000161         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12798_length_5680_cov_9.049600, whole genome shotgun sequence.
ACCESSION   DAOSLE010000161 DAOSLE010000000
VERSION     DAOSLE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12798_length_5680_cov_9.049600"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1790
                     /locus_tag="PK576_09365"
     CDS             255..1790
                     /locus_tag="PK576_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOE61837.1"
                     /translation="MLARVCSGAVYGVHAYTVEIEVNAGRGDPQFVMVGLPDAAVRES
                     KDRVWTAMNNSGFRPTLGRTTINLAPADIRKEGPVYDLPIAAGLLAATGEAELPHLDQ
                     YALIGELALSGEVRRVRGVLPVTLEMRRQGKRGILVPADNAEEAAVAEGIDVYPVRHL
                     REAVDFLAGKLSLHPFRVDLAGLMRRERRHAEDFADVKGQETAKRAIEVAVSGGHNIL
                     MIGSPGAGKTMLARRIASILPEMTLDEAVEATRIHSVAGTLKSHQALILHRPFRAPHH
                     TISDAGMLGGGTHPFPGEVSLAHRGVLFLDELPEFHRNVLEVLRQPLEDGHVTISRAA
                     ATLDFPSQFILVAAMNPCPCGYAGDPKRECRCSSQKIQAYRNKISGPLLDRIDIHIEV
                     PPVRYADLSGLARGDASAVIRERVAVCRARQRERFRDLPRLTCNAAMGSREVQTFCPL
                     SGDAAELLKMAMTELNFSARAYDRIIKVARTIADMAGSEIIGPNHISEAIQYRSLDRL
                     MWV"
     gene            1828..3084
                     /locus_tag="PK576_09370"
     CDS             1828..3084
                     /locus_tag="PK576_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HOE61838.1"
                     /translation="MPPMPRPYYADLHVHSHYSRATSPACTPEGLFRWARLKGVGVCG
                     TGDCTHPAWLTELRAKLIEDAPGLFTLKPALRGEACSGVPEACRGPVRFMLTGEISSI
                     YRRDGACRKIHSLVLLPSFEAAAALNERLAQVGNILSDGRPILGLDPRDLLTMLLECD
                     PRAALIPAHIWTPWFSLLGAKSGFDSPEACFGDLMPHLFAVETGLSSDVPMNRRIRCL
                     DRLTLVSNSDLHSPAHLGRNATVFYGEPAYDTILKGLRTADPALYGGTVDLFPEVGKY
                     FLDGHRACRVRLTPEESLACGTLCPACGKPVTIGVLHRVVELERRQAASARSRAFRTP
                     YRYIVPLPELLSQQLGTAPRSKKVGTAYQALLAQVGPELPLLLDADARTLGALDALGE
                     AIRGVREGRVRREGGYDGEYGSVRVI"
     gene            3081..3605
                     /locus_tag="PK576_09375"
     CDS             3081..3605
                     /locus_tag="PK576_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOE61839.1"
                     /translation="MNLLLHTCCAPCASHCVLTLRELGHTVTLFYTNANIAPHAEFLR
                     RLESVERLAERLAVPLLVEEPDHADWLARVACGLEDASERGPRCAHCFRYSLCRTAAR
                     MRQCGFDGFTTSLSVSPHKHTATLFEAGRAIDAERFLAIDFKKRDGFLRSLQLATEFG
                     LYRQSYCGCEFSRN"
     gene            complement(4193..5608)
                     /locus_tag="PK576_09380"
     CDS             complement(4193..5608)
                     /locus_tag="PK576_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE61840.1"
                     /translation="MPTSVRMRYYERTMTQDRTNNVAAETLVLADGENQQSLLRVRRI
                     KLLVISGPLQGREILVNRDTFTIGSGSHNDLIVEDSTVSKRHCEIVVEQSGYLIRDLE
                     STNGTLVQGVRVSSAHLPPGSEIQLGKTRIVFCPLQEAHDIPLSNREAFGAMLGSSVP
                     MRRIFYLAETYSPADVTVMITGETGTGKEILAEEIHNHSPRHDKPFIVIDCAAISKEL
                     IESELFGHVKGAFTGANADRQGAFELADGGTVFLDEIGDLSPDLQPKLLRVLEKREIK
                     RVGCNKVRKIDVRIISATNRNLANEVNEGRFREDLYYRLSVVHLELPPLRRRREDIPL
                     LIKRFLTDLHGEDALSQLADFDRTTEILKRHEWPGNVRELRNLIELAFYSERRPVDLS
                     AFLSLGSLRAGRKPAEPEVNFVTDRPFKDAKNDLIEEFERTYLNDLLARNKQNISRSA
                     REAGIERAYLQRLIRKYGMRE"
BASE COUNT          871 a         1734 c         1916 g         1159 t
ORIGIN      
        1 acgccagggg gaattcctga ttttcatgaa gtttcatgtg tttcatggtt tcatccaaat
       61 cggggtcggg gtcggtatcg gtatcggtat cgctatcggt atcgtcgacc cgcgacccgc
      121 gacattcgac ccgcggcgat cggcggggat gcccgcgctt ccggcaaacc aagagaggtc
      181 ccgcttttcg ggggttgcgt ctgtcagggg atgagattac actgacgtct cattctcgcg
      241 taaaggagtg gctcatgctt gcccgtgtct gttcaggcgc tgtctatggg gttcatgcgt
      301 ataccgtcga aatcgaggtc aatgccgggc ggggggatcc gcagttcgtg atggtgggtc
      361 tgccggacgc ggcggtgcgg gagagcaaag accgcgtctg gaccgccatg aacaactcgg
      421 gtttccggcc gacgctcggc cgcaccacca tcaacctcgc gcctgcggac atccggaaag
      481 aggggccggt ctatgacttg ccgatcgcgg ccggcctgct ggccgccacg ggcgaggcgg
      541 aacttccgca tctcgaccag tacgccctga tcggcgaact cgcgctcagc ggcgaggtcc
      601 ggcgtgtgcg cggtgtgttg ccggtcaccc ttgaaatgcg ccgtcagggc aagcgcggca
      661 ttctcgtgcc cgctgacaat gccgaggagg ccgcagtggc cgagggcatc gatgtgtatc
      721 cggtgcggca tctgcgtgag gccgtcgatt ttctggcggg caaactgagc ctgcatccct
      781 tccgggtcga ccttgccgga ctcatgcgtc gcgagcgccg ccacgccgaa gatttcgcgg
      841 atgtcaaagg gcaggagacc gccaagcgcg ccatcgaggt ggcggtgagc ggcgggcaca
      901 acattctgat gatcgggtcg ccgggcgcgg gcaagaccat gcttgcgcgg cgcatcgcct
      961 cgatcctgcc ggagatgact ctcgacgagg cggtggaggc cacgcgcatc cacagtgtgg
     1021 cgggcaccct caagagtcat caggctctga tcctgcaccg tccttttcgg gcgccgcacc
     1081 acaccatctc cgacgcgggg atgctgggcg gcggcacgca tccgtttccc ggcgaggtga
     1141 gtctcgcgca ccgcggcgtg ctgtttctcg acgagctgcc ggagttccac cgcaacgtgc
     1201 tggaggtgtt gcgtcagccg ctcgaggatg gccacgtcac gatctcgcgc gcggccgcca
     1261 ccctcgactt tccctcgcaa ttcattcttg tggccgcgat gaacccttgt ccctgcgggt
     1321 acgcgggcga tccgaaacgc gagtgccgct gctcctcgca gaagattcag gcgtaccgca
     1381 acaaaatttc gggtccgctg ctggaccgca tcgacatcca tatcgaagtc ccgccggtcc
     1441 gctatgcgga tctcagcggg ctcgcgcggg gcgacgcctc ggcggtgatc cgtgagcgtg
     1501 tggcggtctg ccgcgcgcgc cagcgcgaac ggttccggga tctgccgcgc ctcacgtgca
     1561 acgccgccat gggctcccgc gaggtccaga ccttctgtcc gctgagcggc gatgccgcgg
     1621 agctgcttaa aatggcgatg accgaactga acttcagtgc gcgcgcctat gaccgcatca
     1681 tcaaggttgc gcgcaccatc gccgatatgg ccggcagtga aatcatcggc ccgaatcaca
     1741 tctccgaagc gatccagtac cgttcgctcg accggctgat gtgggtctga gtcaatcgcc
     1801 cgccccatgc aagaaagaac cgaccccatg ccgcctatgc ctcgcccata ttacgctgat
     1861 ctgcatgtcc actcgcatta ttcgcgcgcg accagtcccg cgtgcacgcc ggaaggcctt
     1921 ttccgctggg cccggctcaa gggggtgggg gtctgcggca ccggggactg cacacacccc
     1981 gcctggctga cggagttgcg cgccaagttg attgaggatg cgcccggcct ctttacgctc
     2041 aagcccgccc tgcgcgggga ggcctgcagc ggtgtgccgg aggcctgccg cggtccggtg
     2101 cgcttcatgc tgaccggcga gatcagcagc atctaccgcc gggatggcgc gtgccgcaaa
     2161 atccacagtc tggtgctgct gccctctttc gaggcggccg ccgcgctgaa cgaacggctc
     2221 gcgcaggtcg gcaacatcct ttccgacgga cggcccattc tcggtctgga tccccgcgac
     2281 ctgctgacga tgctgctgga gtgcgatccg cgcgcggccc tgattccggc gcacatctgg
     2341 acgccgtggt tctccctgct cggcgcgaaa tcgggattcg attcgccgga ggcgtgtttc
     2401 ggcgatctca tgccgcactt gttcgcggtc gagaccggcc tctcctcgga tgtgccgatg
     2461 aaccggcgca tccgttgtct cgatcggctg acgctggtgt cgaactcgga cctgcattcg
     2521 cccgcccatc tcggacgcaa cgccaccgtc ttctacggtg agcctgccta tgacacgatc
     2581 ctcaaggggc tgcgcaccgc tgatcccgcg ctgtacggcg gcacggtgga tctttttccc
     2641 gaggtgggga aatatttcct cgatggccac cgcgcctgcc gtgtgcgcct gactccggag
     2701 gagagcctcg cctgcggcac cctctgtccg gcttgcggca aaccggtcac catcggcgtg
     2761 cttcaccggg tggtggaatt ggagcggcgt caggccgcat cggcccgctc ccgcgctttc
     2821 cgcacaccct accgttacat tgtgccgctg cccgaactgc tgtcgcagca gcttggcacg
     2881 gcaccccgtt ccaaaaaagt cggcaccgcg tatcaggcgc tcctcgcgca ggtcggaccc
     2941 gaactgccgc tgctgctgga tgccgatgcg cgaactctcg gcgccctcga cgcgctgggc
     3001 gaagccatcc gtggcgtgcg tgagggccgg gtgcgccggg agggcgggta tgacggcgaa
     3061 tacgggagcg tgcgcgtgat atgaacctgc tgctccacac ctgctgcgcg ccctgcgcga
     3121 gtcactgcgt cctgacgctt cgggagcttg ggcatacggt gaccctcttt tacaccaacg
     3181 cgaacatcgc gccgcacgcg gagtttctgc gccgtctgga gtcggtggag cgtctggcgg
     3241 aacggcttgc cgtgccgttg ctggtggagg agccggacca tgccgactgg ctggcccgcg
     3301 ttgcctgcgg gctggaggac gcgtccgagc ggggaccccg ctgcgcgcac tgtttccgct
     3361 acagtctctg ccggaccgcc gcgcgcatgc ggcagtgcgg gtttgacggg ttcaccacct
     3421 cgctgtccgt gagtccgcat aaacacacgg ccacgctgtt cgaggcgggc cgcgcgatcg
     3481 atgccgagcg gtttctcgcg atcgatttca aaaagcgcga tgggtttctg cgcagtctgc
     3541 agttggcaac ggagttcggg ctgtaccgcc agtcgtattg cggctgcgag ttcagccgca
     3601 attaaaccga tcccatcgct atcgggatcg ggatcgggtt tcagggtttc agggtttctg
     3661 ggtttcaggt ttctggtttc tgggtttcag ggtttctggt ttctgggttt catctaaatc
     3721 ggggtcgggg tcgggatcgg tatcgctatc gggaacgtcg acccgcgacc cgcgacattc
     3781 gacctgcggc gatcgacagg gacgcccgcg tttccgacac cgcaggtcgc ttttcaaggt
     3841 ttctggtttc tgggtttcgg gtttcagggt ttctggtttc tgggtttctg ggtttctggg
     3901 tttctggttt ctgggtttct ggtttcaggg tttctggttt cagggtttca gggtttcaag
     3961 gtttcagggt ttcatccaaa tcggggtcgg gatcgggttt cagggtttca aggtttctgg
     4021 tttcagggtt tcatctaaat cggggtcggg atcgctatcg ggatcgggat cgggaacgct
     4081 atcgggatcg atactggcgc gggaaagtcg ggagcgcggg cgtccccgcc cgctggtagg
     4141 gacggttccc cgaaccgtcc gcggacgcct cggggatgcg tccctgcccg gctcactcgc
     4201 gcatgccgta cttgcggatc aggcgctgga ggtaggcgcg ctcgatgccg gcttcgcggg
     4261 cggagcgcga gatattctgc ttgttgcggg cgagcaggtc gttgaggtag gttcgctcga
     4321 actcctcgat caggtcgttc ttcgcgtcct tgaagggacg gtcggtcacg aaattgacct
     4381 cgggctcggc gggcttgcgt ccggcgcgca ggctgccgag gctgaggaag gcgctgaggt
     4441 ccacggggcg gcgttcgctg tagaaggcca gctcgatcag gttgcgcaac tcccgcacgt
     4501 tgccgggcca ctcgtggcgc ttgaggatct ccgtggtgcg gtcgaaatcg gcgagttggc
     4561 tgagggcgtc ctcgccgtgc aggtcagtga ggaagcgttt gatcagcagg gggatgtcct
     4621 cgcggcggcg gcgcagcggg ggcagctcga ggtggaccac cgagaggcgg taatagaggt
     4681 cctcgcggaa gcggccctcg ttgacctcgt tcgcgaggtt gcggttggtg gcggagatga
     4741 tgcgcacgtc gatcttgcgc accttgttgc agccgacgcg cttgatctcg cgcttttcga
     4801 gcacgcgcag cagcttgggc tggaggtcgg gcgagaggtc gccgatctca tccagaaaga
     4861 ccgtgccgcc gtcggccagt tcgaaggcgc cctggcggtc cgcgttggca ccggtgaagg
     4921 cgcccttgac atggccgaac agctcgctct cgatcagctc tttggagatc gcggcgcagt
     4981 cgatcacgat gaacggcttg tcgtggcgcg gggagtggtt atgaatctcc tcggcgagaa
     5041 tctccttgcc ggtgccggtc tcgccggtga tcatgaccgt gacgtcggct ggcgagtagg
     5101 tctcggccag atagaagatc cggcgcatcg gtaccgagct gccgagcatg gcgccgaagg
     5161 cctcccgatt ggagaggggg atgtcgtggg cttcctgaag ggggcagaag acgatgcggg
     5221 ttttgccgag ttggatctcc gaccccggag ggaggtgggc ggaggagacg cgcaccccct
     5281 gcacgagggt gccgttggtg ctctccagat cgcggatgag gtagccgctc tgctccacca
     5341 caatctcgca gtggcgcttg gagacggtcg aatcctcaac gatcaggtca ttgtggctgc
     5401 ccgaaccaat ggtgaaggtg tcgcggttga ccagaatttc gcgtccctgg agcggcccgc
     5461 tgatcacgag cagtttgata cggcgcacac gcagcaggct ctgctggttc tccccgtccg
     5521 cgagcaccag agtctcggcg gccacattgt tggtccggtc ttgagtcatc gtgcgttcat
     5581 aataacgcat gcgtacggat gtaggcaacc ctgtttttct tttaataggg cttgcccgac
     5641 ctcttttggg catggtaagc tataacaatc gcggcatagt
//
LOCUS       DAOSLE010000162         5647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12882_length_5647_cov_8.273069, whole genome shotgun sequence.
ACCESSION   DAOSLE010000162 DAOSLE010000000
VERSION     DAOSLE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12882_length_5647_cov_8.273069"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PK576_09385"
     CDS             complement(<1..671)
                     /locus_tag="PK576_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE61841.1"
                     /translation="MNTAVAVFSALSPERVLEAVECGLRCRCTGLLRPLPSYINRVYT
                     VELETGETVVAKFYRPGRWTRAAIIAEHDLVLACAADEIPVIAPLPLADNKGTVGDAD
                     GIAFALYPRRGGRPIEPAEPADALWGRLGALLARVHRVGARAPAPDRLTLHPLSTTTE
                     DLAYLLDSGFLEAREAGRLEAFAAALFERIVPQFEELGGEVIRLHGDCQHTNVLERPE
                     TGLFL"
     gene            complement(668..3064)
                     /locus_tag="PK576_09390"
     CDS             complement(668..3064)
                     /locus_tag="PK576_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE61842.1"
                     /translation="MRRLLKDEGVRLACVSAGGSVMAGVWLAEGLRVLDTFAGRTAPA
                     LAACAAVGVVGLTTGVLAAARPARRVRHAARWMSGTLLMCAVALVTLLAILPAMSAGW
                     QRLVGDLSRTFGDYLLAVGKTALVLFFVPAVLAGVAARFALAAVRTERTGAGQELTLC
                     LLLGLLPAVFGFGFGRTVLASAVDTETLLRLGALWCGALASFLVHGRGLSAALPFAAV
                     AVLLAAVRPATGPGVLAEGLFARLVHRDSGFARGTPVFAHRTRHHTVALFRDDDYETV
                     LTLDGRPVLFGNRFHTARTLTGLLPALLHPASAKVVVLGPEAGLYLPFVFRAGVTNVA
                     VAGSDRAVVELALAADRLVTGGDAVDVRQVKRRVRLTRRAGYDLIFLAGEPLWLRGTR
                     GSVDAKLFKRCRAALSDDGIVALHLDARALPMGRFVSVARRFTAVFSEVQVWQTGSDD
                     WLLIGATRPFEVRAERMLERFERREVMRDCARAGIRSLPEILACRVGNGPAWAAWLER
                     ASGSSVKAGIWRAPRTAFSSGAADRLRAASLDDLRPADLDWLRPGALDDATRAALLDK
                     AGVCREAHRLAASAFLLREQGRGEAALQAAREAARLHPGGVLLTPFGESLDLEGRRRI
                     AIGDFKGALKCYENLLSLQTGLARAHYGMAYCLRATGEAESAYLHFARAVAAAPTQIS
                     YRLAFAQACSAVGEYAEADRQYEAALRQEPDNPELLFRFARGLALAARPEKDFARAIR
                     LAERACVVTRWRHSEYAYGLADLYLDAGRVLEGMGLKRRLKEGLISEPGVPDGKGGTR
                     "
     gene            complement(3061..3894)
                     /locus_tag="PK576_09395"
     CDS             complement(3061..3894)
                     /locus_tag="PK576_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOE61843.1"
                     /translation="MNMTKINAVLGEYVLPFLGICAWLIFSLWFASFLARLLYNALSR
                     KVGPDTARVISKTVKYVLVVLFTLSLVRGAGVDVSAVLGAAGVAGVAIGFASQTSLSN
                     LISGLFLLLERPFKVGDWIEVDGSFGFVHSVELLSSYIRTFDNRMVRIPNEMLAKSKL
                     INHTHYPIRRLDLTIHVSCREDAARVMRLFREVVIANPLCFNEPEPMIVLQGFGDSSM
                     EFLVAVWTERSELRTTRTSLLRDIKERFDQEGIALPYHRIRMAECDGMAVPEGSGKEV
                     T"
     gene            complement(3917..4702)
                     /locus_tag="PK576_09400"
     CDS             complement(3917..4702)
                     /locus_tag="PK576_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61844.1"
                     /translation="MNSLWGNHICEEGRDYTASVGCLRLTVRRIRQGWLVASRHTTDS
                     AFESLREVPAVPEQTEWMRFITPEAAPKLHLTPILPDRSVVSQSEAHIEIAPKHSGLF
                     YVTIPVWVQVRAGEISPVTLCEFPSQILSKTWFGHPSEEGEICYALTTRARQELAELT
                     DIQGRVICPLTLDNMTSEPLVFGQLLLSVRHMSVYQTQDGRLWTNECRMGFAGRSLPA
                     SVTFGRTVPAVAQQARWVSGPREAPASGLSARVIANAIFRQPF"
     gene            5158..>5647
                     /locus_tag="PK576_09405"
     CDS             5158..>5647
                     /locus_tag="PK576_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61845.1"
                     /translation="MMRRGRRGDSTMDGNLFCGGTEGGGAASAGLPCLLILAAYALCL
                     AARVWERGGLRQFRAGLRGTWASCPPALRAVMAGLLLACVWDGRCTKPENGGGGTNSP
                     AAPAQQQPPAPEGQAAGTPAAAAPGQTGQASGGAAPMPAGAPAPALPTLADGTNCWWI
                     AEG"
BASE COUNT         1147 a         1996 c         1691 g          813 t
ORIGIN      
        1 atcagaaaca agccggtctc gggcctctcc agcacattgg tgtgctggca gtccccatga
       61 agccggatga cctcaccccc gagttcttca aactgcggca cgatccgttc gaacagagct
      121 gcggcaaagg cttccaaacg cccggcttca cgcgcctcca gaaacccgct gtccagcagg
      181 tacgcgagat cctctgttgt ggtggataac ggatgcagcg tcagccggtc tggcgcgggc
      241 gcccgggcgc caacccgatg gacacgcgcc agcagggcac caagacgccc ccagagcgcg
      301 tcggctggct ccgctggctc gatcggcctc ccgccccgcc gcggatacag ggcgaacgcg
      361 ataccgtccg catcccccac cgttcccttg ttgtcggcga gaggcaacgg cgcgatcacg
      421 ggaatctcgt cggcggcaca ggccaacacc aagtcgtgct ccgcaatgat cgccgcacgt
      481 gtccagcggc cgggacggta gaatttggcg accaccgttt ccccggtctc caattcgacc
      541 gtgtacacgc ggttgatgta actcggcaag ggacgcaaca gcccggtgca acgacagcgc
      601 aaaccgcact ccaccgcctc cagcacacgt tccggcgaga gcgcgctgaa caccgcaaca
      661 gccgtattca tcgcgtccct ccctttccgt cgggaacgcc gggctcggag atcaacccct
      721 ccttcagacg ccgtttcagg cccatgccct ccagtacgcg gccagcgtcc agatagaggt
      781 ccgcaagccc ataggcgtac tctgaatgcc gccagcgcgt caccacgcag gcgcgctcgg
      841 ccaaacggat ggcccgcgcg aaatcttttt cgggtcgcgc ggcgagggcg agcccccggg
      901 cgaaacggaa cagcaattcc ggattgtcgg gttcctgccg caacgccgcc tcatattgcc
      961 ggtccgcctc cgcatactcg cccacagcgg aacaggcctg cgcgaaagcc agacggtaag
     1021 aaatctgcgt gggtgccgca gccaccgcac gcgcgaaatg gaggtatgcg gactccgcct
     1081 caccggtggc ccgcagacag taggccatgc cataatgagc gcgcgccagc cctgtctgaa
     1141 gcgaaagcag gttctcgtaa cacttcaacg cgcctttgaa atcgccgatc gcgatccgtc
     1201 ggcgcccctc gagatccagc gattcgccga agggagtcaa caagacgccg ccaggatgaa
     1261 ggcgcgccgc ctcgcgtgcc gcctgcagag cggcctcgcc ccgaccctgt tcgcgcaaga
     1321 ggaatgcgga agccgccaat cggtgcgcct cccggcagac accggccttg tccagcaggg
     1381 ccgcgcgcgt ggcgtcatcg agtgcgcccg gccgcagcca gtcgagatcc gcgggccgta
     1441 agtcatccag cgacgcggcg cgcaaccgat ccgccgcccc tgacgaaaac gccgtccgcg
     1501 gcgcgcgcca gatcccagcc ttaaccgaag aacccgacgc gcgttccagc caagccgccc
     1561 atgcaggacc gttgccaacc cggcacgcca gaatctcagg cagactccgg atccccgccc
     1621 gtgcgcaatc gcgcatgacc tcacgccgct cgaaccgctc gagcatgcgt tccgcacgca
     1681 cctcgaacgg ccgcgtggct ccgattaaca gccagtcgtc ggaaccggtc tgccacacct
     1741 gcacctcgct gaacaccgcg gtgaaacgcc gggccaccga aacgaaccgc cccatcggca
     1801 aggcccgcgc atccagatgc aacgccacga tcccgtcgtc cgagagggcc gcgcggcaac
     1861 gcttgaacag tttcgcgtcc accgagccgc gagtcccgcg caaccacaac ggttcgccgg
     1921 ccagaaaaat gagatcatac cccgcccgcc gtgtcagccg gaccctgcgc ttcacttgcc
     1981 gcacatcaac cgcatccccg cccgtaacaa gccggtccgc agccagagcc agttccacga
     2041 ccgcccgatc ggaccccgcc accgccacat tggtgacacc cgcacggaaa acgaatggca
     2101 gatacagccc cgcttccgga cccagcacca ccactttggc actggcggga tgcagcagcg
     2161 caggcagaag cccggtcagt gtgcgcgccg tgtggaaacg gttaccgaac agcaccggcc
     2221 gaccgtcgag ggtcagcacg gtttcgtaat cgtcgtctcg aaacaaagct acggtatgat
     2281 ggcgcgtccg gtgcgcgaac accggcgtgc cgcgcgcgaa accgctgtcc cggtgcacca
     2341 gacgcgcgaa cagaccctcc gccagcactc ccggccctgt agcgggccgc actgccgcca
     2401 acagcactgc caccgccgcg aacggtaacg cggcactcaa tccccggccg tgcacgagga
     2461 acgaggccag agcaccgcac cacaacgcgc ccagacgcaa caacgtttcc gtatcgacgg
     2521 cggatgccag cactgtccgg ccaaacccaa agccgaacac cgcaggcaac agaccgagca
     2581 gcaggcacag cgtcagctcc tgtccggcgc ccgtgcgctc tgtccggaca gccgccaacg
     2641 caaagcgggc ggccacaccg gccagcaccg ccggaacgaa aaacagcacg agggccgtct
     2701 tgccgactgc caacaggtag tcgccgaacg tgcgcgacaa gtcacccacc agacgctgcc
     2761 atcccgcgga catcgcgggc aagatcgcga gcagcgtcac caacgcgacc gcgcacatca
     2821 acaacgtgcc gctcatccat cttgcggcgt gccgcacccg acgcgccgga cgggcagcgg
     2881 cgagaacccc tgtcgtcaag ccgacaaccc ctacggcggc acaggcggcc aacgcaggcg
     2941 cagtgcggcc cgcaaaggta tccagcaccc gcaacccctc ggccagccac acgccagcca
     3001 tgacactgcc gcccgcgctc acgcaggcca accgcactcc ctcatccttc agaagccgcc
     3061 tcatgtaacc tctttcccgc ttccctccgg cacggccatg ccatcgcact ccgccatgcg
     3121 gatccggtga tacggcaacg cgatcccttc ctgatcgaac cgttctttga tatcccgcaa
     3181 cagggatgtc cgcgtggtcc gcagttcgct ccgttcggtc cagaccgcca ccagaaactc
     3241 catcgacgag tcgccgaacc cctgcaagac gatcatcggt tcaggctcgt tgaagcacaa
     3301 cggattcgcg atcaccacct cacggaagag ccgcatgacc cgcgccgcat cctcccggca
     3361 cgaaacgtgg atcgtgagat ccagccgacg gatcggataa tgggtgtgat tgatgagctt
     3421 ggacttggcc agcatctcat tggggatacg caccatacgg ttatcgaacg tacggatgta
     3481 ggaggagagc agttccacgc tatggacaaa gccgaacgag ccgtccacct caatccagtc
     3541 ccccaccttg aacggccgct ccaacagaag gaacaggccg ctgatcagat tcgaaagact
     3601 ggtttgggag gcgaagccaa tggcgacacc cgcaacgccc gcggcaccca gcaccgccga
     3661 aacatccaca cccgcgccgc ggaccagcga gagcgtaaac aacaccacaa gcacatattt
     3721 gaccgttttc gaaatcacgc gcgcggtatc gggccccact tttcgggaga gcgcgttata
     3781 cagcagacgt gccagaaacg aggcgaacca gagactgaag atcaaccagg cgcagatgcc
     3841 gaggaacggg agcacatact cccccagcac ggcattgatt ttcgtcatgt tcatgccctt
     3901 ccctcccttt ttcgactcag aacggctggc ggaaaatcgc gttggcgatc actctggcac
     3961 tcaggccgga ggccggggct tcgcggggac ctgagaccca acgagcctgc tgcgcgacag
     4021 ccgggaccgt gcgaccgaag gtcacggacg caggaagaga ccggcccgcg aaccccatgc
     4081 ggcactcgtt cgtccacaac cgcccgtctt gggtctgata gacactcatg tggcgcacac
     4141 tcaataaaag ttggccgaac accagcggct ccgaggtcat gttgtcaaga gtcagcggac
     4201 agatcacgcg gccctggata tcggtcagtt ccgcaagctc ctgacgcgcg cgggtggtca
     4261 gggcataaca gatttccccc tcctccgaag gatgaccaaa ccacgttttg gaaagaattt
     4321 gggaagggaa ctcgcacagc gttacgggtg aaatctcacc ggcccgcacc tgcacccaca
     4381 cgggaatggt gacgtaaaac aggccgctgt gtttcggcgc aatctcgata tgcgcctcgg
     4441 actggctgac caccgaccgg tcaggcagaa tcggcgtcag atgcagcttc ggcgcggcct
     4501 ccggggtgat gaagcgcatc cattcggtct gttccggcac agccggaacc tcacgcagcg
     4561 actcgaaggc actgtcggtc gtatgccgcg acgcgaccag ccagccctgc cgtatgcgcc
     4621 gcacagtcag gcgcaggcat ccgaccgagg cggtgtaatc gcgcccctct tcgcagatat
     4681 gattccccca cagactattc atgcggaaag tatagcaggt tccgtgcgag cggccaatgt
     4741 cagtgatcat taataaccgg gaacctcaat gtgacaagtg aagttcccgg ttgttttccg
     4801 aaacagcggt tgacgccgat gtataaacgg cgtatgcttg aaccatctca ggaaacggtt
     4861 gtcccccgtc ccgcatccag gcggtgacgg aaaccgggaa aagacgggga aacgccgtct
     4921 gcggttttgc ggacgaacgc tgactgagag acgacagcca cagacagttt gattttgcgc
     4981 ttacgcgcat taacgcttca gagaagaaac cgccagtttt tatagcgaag cgagatgccc
     5041 gggggtgcgc cgcctggcgc gcctccagtc tcgtgtttcg ctatgggcgc ctggcggtgc
     5101 cttctctgag gcacgggctg gagtccgcgc cctttttgat ggggcgcggg ttccagcatg
     5161 atgcggcgcg ggcggcgggg ggacagcacg atggacggga atcttttttg cggcgggacg
     5221 gaaggcggcg gggcggcgtc tgcgggcctg ccgtgccttc tgatcctggc ggcgtacgcg
     5281 ctgtgcctcg cggcgcgcgt atgggagcgg ggcgggctcc gccagttccg cgcgggcctg
     5341 cgcgggacgt gggcgtcttg tccgcccgcg ttgcgggccg tcatggcggg cctgctgctg
     5401 gcctgcgtgt gggacgggcg ctgcacaaag cccgaaaacg gcggcggcgg gacgaacagc
     5461 cccgccgcgc ccgcgcagca gcagccgccc gcgccggaag ggcaggcggc gggaaccccg
     5521 gccgccgccg cgccgggcca gacgggccaa gcgtccggcg gcgccgcccc catgcccgcg
     5581 ggcgcgcccg cgcccgcgct cccgacgctg gcggacggca ccaactgctg gtggatcgcc
     5641 gaggggc
//
LOCUS       DAOSLE010000163         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_12921_length_5633_cov_8.949086, whole genome shotgun sequence.
ACCESSION   DAOSLE010000163 DAOSLE010000000
VERSION     DAOSLE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12921_length_5633_cov_8.949086"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PK576_09410"
     CDS             <1..329
                     /locus_tag="PK576_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61846.1"
                     /translation="GGAVYARQVTLTQNDAKGPVVLDLGGVGVTCEVAVNGEPARVLV
                     CPPWRVDLTDRVKAGDNQLAITVYNTLNNHYQTIPTRYRTPVEATPSGLLGPVTLHFG
                     AKAVPR"
     gene            470..3268
                     /locus_tag="PK576_09415"
     CDS             470..3268
                     /locus_tag="PK576_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61847.1"
                     /translation="MKNKSSLLILASLLFAGCATPMRTVFVAPDGNDANPGTKSRPFA
                     TLPRALSSVRDLRQAGLPSGPVTVYLRGGTYAVRKPILFTPDDSGTSNAPLTVASYPG
                     EQAVLSSGERLSGTWTQTPGKPYWQLDIPRARNSGWVFYSLFVNGQSRERARFPNWGG
                     KVLRAEGREPGGDPRQALRYFAGDVNPAWSNPTDIDVVLLCSWTPTIHRINEIVPERR
                     AVRFFSAHNRPVDAWERNFRYYLSNVFEALDQPGEWYLNRHTGTLYYYPMPGEEMASA
                     EVIAPVLRSNMVTFEGDPESGRLIEHLHFRDLAFRHLDGDMDKHNGVYRQGHMFLSSA
                     VVAHGLRHASFERCEFAQLGEYALELADGCRDVTVRQCHLWDLGAGALQLGVTDLRTL
                     LHGRDTSPNPDRPRGKTTGSKTPPHPMEDPLSNPRFVTGLVIDNNCIHRLGTIWHGCY
                     GIVNRFASHSRITHNEIYDIHWDAIGLDARWNWKGENYSSGTVVAYNHLHHLGLRYHT
                     DAAGIYQYGPLDTHIHHNLIHDAVAYPYICGFAGIYLDEQSRGAVVENNLVYNTEWFA
                     YFQHKGTDNIFRNNIGAFARDGFLGRGSLNETWKTNYLEASRNLYISSNAVAIRSGWQ
                     PGLRAPVLTRNLYHTLTTNTLTFAGKEFAEWQAEGYDKDSVTADPGCRNPAAFDFSLE
                     PNAPACRLIGFTPFTTEIRKAGLYGDKAWRTTASRYPARVPSAVWDEVDFRKLGHAFE
                     LDFNLMKPGDGPDVFRVTDQKGAGFAVTREVAGMTGAHCLKATDKPGLSQSFYPYMHL
                     APRGLKSGRVTFTFALRHPERGAASCIVEFRGAGQTDTGPSIAVGRDGAIRANGKPVG
                     TLMPGAWNRFEIAFTLGSNRTDGYTLSIRTPEGEKTLSLPFGKKTFEQVGWLGIILPD
                     HTEGCLYLDAITLAVDES"
     gene            complement(3396..4901)
                     /locus_tag="PK576_09420"
     CDS             complement(3396..4901)
                     /locus_tag="PK576_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61848.1"
                     /translation="MTKVWRSVLLIGVGLLVRDAWTAEQDPADLLAETILRKVDTVLA
                     PVRERASGLRPAAPDKALVRTEVWREWRMDESGALCQVCVTGEIWTAAIQQALETHGC
                     VRLPERGMPYYIDAPILLSSGQLFRADPQAEIRLKPNSNTCMIRNRNVVGMREGGVIP
                     PVPYDTGVVVEGGIWTTLRIGRKESNGNNRGHPSRTDRAFESGHGVVLMNHADGLILR
                     NLTIRQSQVHGLQLSNVRHFHVEGIRFEEHGRDGVHVHGASEYGVIRDVRGATYDDFI
                     ALNAWDWRNTTPAYGPIRHVLVEEVGGSAEDPLRRAGSAAIRLLPGTRTWADGSKTAC
                     PIEFCVLRRLTAIQTFKVYDQPNLELGRDRDFSDPIGTVRDLFIRSLNCPAPCSIEIA
                     AHVSNFSVEDVTFRFPPAEGYRLIRVGPKSGTYKPRSTDPSTWVELFSPDKDITVRAL
                     SLRRVRVETAGKCAEIEDPERRFVVFEDQRPNPDYPKTTPKGGRGRVIRLP"
BASE COUNT         1088 a         1848 c         1697 g         1000 t
ORIGIN      
        1 ccggcggcgc ggtctatgcg cggcaggtga cgctcacgca gaacgacgcg aaggggcccg
       61 tcgtgctcga tctcggcggc gtcggcgtca cctgcgaggt cgccgtcaac ggcgaacctg
      121 cgcgcgtgct ggtctgcccg ccgtggcggg tggacctcac ggaccgcgtg aaagccggcg
      181 acaaccagtt ggctatcaca gtctacaaca cactcaacaa ccactatcag acgatcccga
      241 cccgttaccg cacacccgtc gaagccacac cttccggcct gctcgggccg gtgacgctgc
      301 acttcggagc gaaggcggtg ccgaggtaaa ccaagccccg gggttccggg cgaaaacgag
      361 agggaaactg gaatggtcaa cgtaaagaca agccgggatt tccgacagtt tcgcttcggc
      421 ggggagaaaa ccggaatgaa caggatttat gattaaggac aggacaccta tgaaaaacaa
      481 gagttcgctt ctcattctcg cctcccttct ttttgccggc tgcgccacgc cgatgcggac
      541 ggtcttcgtc gcgccggacg gtaatgacgc caaccccggc acgaagtccc ggccctttgc
      601 gacgctgccg cgcgccctgt ccagcgtgcg cgatctacgc caggccggac ttccgtccgg
      661 accggtcacc gtatatctgc gcggcgggac ctatgcggtg cgcaagccga tcctcttcac
      721 gccggatgat tccggaacct ccaacgcgcc gctgacggtt gcctcctatc ccggcgaaca
      781 ggcagtccta agcagcggtg aacggctcag cggcacatgg acgcagacgc cgggcaaacc
      841 ctactggcaa ctcgatattc cgcgtgcccg caacagcgga tgggtttttt attcgctctt
      901 tgtcaacggc caatcgcgcg aacgtgcccg cttcccgaac tggggcggaa aagttctgcg
      961 cgccgagggc cgcgagcccg gcggcgaccc gcgccaggcg ctgcgttatt tcgcgggtga
     1021 cgtgaacccg gcctggtcga atccgaccga tatcgacgtg gtgctgctct gctcgtggac
     1081 gcccacaatc caccgtatca acgagatcgt tcccgagcgc cgcgcggtgc gctttttcag
     1141 cgcccacaac cgtcccgtcg atgcctggga gcgcaatttc cgttactacc tcagcaacgt
     1201 gttcgaagcg ctcgaccagc cgggcgagtg gtatctcaat cgccacaccg gaacgcttta
     1261 ctattatccc atgccgggcg aggaaatggc ttcggctgag gtcatcgcgc cggtcctgcg
     1321 ctccaacatg gtcacctttg agggcgatcc cgaaagcggg cggcttatcg aacacctgca
     1381 tttccgcgac ctcgcgttcc gccaccttga cggcgacatg gacaaacaca acggcgtcta
     1441 ccgccagggc cacatgttcc tgtcctctgc ggtcgtcgcg cacgggctgc gccatgcctc
     1501 gttcgagcgc tgcgagttcg cgcagctcgg cgagtacgcg ctggagttgg ccgacggctg
     1561 ccgtgacgtg acggtgcggc agtgccacct ttgggacctc ggcgcgggcg cgctccaact
     1621 cggcgtcacc gacctccgta cgctgctgca cggccgggac acttccccga acccggaccg
     1681 tccgcgtggc aagacaacgg gttcgaagac cccgccccac ccgatggagg atcctttgag
     1741 caacccgcgt ttcgtgacgg gcctcgtcat cgacaacaac tgcatccacc gcctcggcac
     1801 gatttggcat ggctgctacg ggatcgtcaa ccgcttcgcc tcgcactcgc ggatcacaca
     1861 caacgagatc tacgatatcc actgggacgc catcggcctc gacgcgcgct ggaattggaa
     1921 aggcgagaac tattcctccg gcaccgtggt ggcctacaac cacctgcacc acctcggact
     1981 gcgttaccat accgatgcgg cggggatcta tcagtacggt ccgctcgaca cgcacatcca
     2041 ccacaacctc atccatgacg ccgtggccta tccctacatc tgcgggtttg ccggcatcta
     2101 tctggatgag cagtcgcgtg gcgcggtggt ggaaaacaac cttgtttaca acaccgagtg
     2161 gttcgcctat ttccaacaca agggcacgga caacatcttc cgcaacaata tcggggcctt
     2221 cgcccgcgac ggctttctcg gtcgcggcag cctgaatgaa acctggaaga ccaattatct
     2281 ggaggccagc cgcaatctct acatcagcag caatgcggtg gcgatccgca gcggctggca
     2341 acctggcctc agggcgccag tcctcacgcg caatctctac cacacactca ccaccaacac
     2401 gctgactttt gcgggcaagg agttcgcgga atggcaggcc gaggggtatg acaaagattc
     2461 ggtgaccgcg gatccgggtt gccgcaaccc cgccgcattt gatttctcgc tcgagccaaa
     2521 cgcacccgcc tgccgcctga tcgggttcac gccgttcact accgaaatcc gcaaggccgg
     2581 tctttacggc gacaaggcct ggcgcaccac ggcgtcccgt tatccggcgc gtgtgccctc
     2641 cgccgtgtgg gacgaggtgg acttccggaa actgggtcat gccttcgaac tggacttcaa
     2701 cctgatgaaa cccggcgacg ggccggatgt cttccgggtg accgatcaga agggggcggg
     2761 gttcgccgtc acgcgcgagg tcgccggcat gaccggcgcg cactgcctaa aagccacgga
     2821 caaaccggga ctctcgcaat cgttctaccc ctacatgcat cttgcgccac gcgggctgaa
     2881 gagcgggcgc gtcaccttca ccttcgcgct ccggcatccg gagcgtggcg cggcctcctg
     2941 catcgtcgag ttccgcggcg cgggtcagac cgacaccggg ccctcgatcg ccgtagggcg
     3001 cgacggcgcg atccgggcga acggcaaacc ggtcggcacg ctcatgcccg gcgcatggaa
     3061 ccggtttgaa atcgcgttca cgctgggctc aaaccggacg gacggctata cactcagcat
     3121 ccgtacccct gaaggcgaga aaacgctgtc cctgccgttc ggcaaaaaaa cgttcgaaca
     3181 ggtgggctgg ctcggcatca ttctgccgga tcacaccgaa ggctgtctct atctcgacgc
     3241 gatcacgctc gcggtcgatg agtcctgaac ccgggccaaa cggagacccc gcgtttcacc
     3301 cctggccctc cggattacgg gcaaacagac ggtctcctcc agcacggcgg gacggctacg
     3361 ctacgcgccg taggcgcgcc actttggttt caggtttagg gcaaccggat gacgcggccg
     3421 cggccgccct tcggggtggt tttcggataa tcgggattgg gccgctgatc ctcaaacacg
     3481 acgaagcggc gctcggggtc ctcaatctcc gcacacttgc ctgcagtttc gacacggaca
     3541 cggcgcaggg agagagctcg gacggtgatg tccttatccg gggaaaacag ttccacccaa
     3601 gttgaggggt ccgtcgagcg gggcttgtac gtgccggatt tcgggccgac ccggatcagg
     3661 cggtatccct cggcaggcgg aaaccggaat gtgacatcct ccaccgagaa attgctgaca
     3721 tgcgcggcga tttcgatgct gcaaggggca gggcagttca gcgaccggat aaagagatcg
     3781 cgcaccgtcc cgatcggatc actgaaatcc cggtcgcggc ccagttccag attcggctga
     3841 tcatagacct tgaaggtctg gatcgcggtg agtcgacgca acacgcagaa ctcgatcgga
     3901 cacgcggtct ttgagccgtc cgcccaagtg cgcgtcccag gcaggagacg gattgccgcg
     3961 cttccggccc tccgcaacgg atcctccgca ctgccgccga cctcttccac cagcacatgc
     4021 cggatcggtc cgtaggctgg cgtggtgttg cgccagtccc aggcattgag cgcgatgaaa
     4081 tcatcgtagg tggccccgcg gacatcgcgg atcaccccgt actcagaggc accgtggaca
     4141 tgcacgccat cgcgcccgtg ctcttcgaaa cggatcccct ccacgtgaaa gtggcgcaca
     4201 ttcgagagtt gcagcccgtg aacttgggac tgccggatcg tgaggttccg gaggatcagt
     4261 ccgtccgcat gattcatcag caccacaccg tgtccgcttt cgaacgcgcg atccgttcgg
     4321 gacggatggc cgcggttgtt gccgttgctc tccttgcgtc cgatgcggag cgtggtccag
     4381 atgccgccct cgaccacgac gccggtatcg tacggcaccg gcggaatcac gccgccctca
     4441 cgcatgccga ccacgttccg gttgcggatc atacacgtgt tcgagttcgg tttcagacgg
     4501 atctcggctt gggggtctgc acggaagagt tggccggagg agagcaggat cggtgcgtcg
     4561 atgtaatacg gcatcccccg ttccgggagc cgcacgcatc cgtgcgtctc gagcgcctgt
     4621 tgaatcgcgg cagtccagat ctccccggtc acgcatacct gacacagcgc accggactca
     4681 tccatacgcc actcccgcca gacctctgtg cgaacaagcg ccttgtccgg tgcggcagga
     4741 cgcagaccgg acgcgcgctc acgaaccggc gcgagaaccg tgtcaacttt ccgaagaatg
     4801 gtttccgcca acaggtcagc cggatcctgt tcagccgtcc aggcatcccg gacaagcagg
     4861 cccacaccga tcaacagcac gctcctccat accttcgtca tcgtcccccc ttttttgatg
     4921 aaggttgcct gcgtaaccgt tgggatctcc ccgcacacac gcgacaaccg taatgactgt
     4981 atccgaacct ccccgcaggg tcaagatggc cagcatgcga agagcgtgaa tccagatcga
     5041 atacatacgg ccggctgatg gaaaaagctt tgttttcgac aaaagcgggg ttgcgcggga
     5101 ttcggcaaaa gagtaaaatg aaagcgtgaa cgaaaggagc gttcccgtct tgtgcgggga
     5161 gaaacacggc atgtagaggg tgattccaga ccggttccga ttttgcgctt tcgcgcatta
     5221 cgcttctcaa gaaaaccgct aattttcatc aagcgaagcg agatacgcgg ggacgcgccc
     5281 ctgtggcgcg gcccctgtct cgggcttcgc ttagggtctt agcggtccct cttgaggggc
     5341 acgaaccggg gtccgcgccc tttactttat atgccgggaa agggacggca atggaaagcg
     5401 ataggacaag taacagaaag aaaagccttg tgcttcttgt cgcggcggcg tgcgcgtccg
     5461 cgggagtcat cttcgcatcg agacacgcgt tggcacagga aaacgcggag caggcacccg
     5521 cctcccgccc cgcttccgcg tgcaagacgc ccgccacccc gctggagatg gctctgaagc
     5581 tggagcgcga gctggcgcgg ggcgcggggg cgctcatgcc ggacccggag cgg
//
LOCUS       DAOSLE010000164         5483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13337_length_5483_cov_8.911570, whole genome shotgun sequence.
ACCESSION   DAOSLE010000164 DAOSLE010000000
VERSION     DAOSLE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13337_length_5483_cov_8.911570"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1978)
                     /locus_tag="PK576_09425"
     CDS             complement(<1..1978)
                     /locus_tag="PK576_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61849.1"
                     /translation="MTHSKRWLLVLSVSAMACGAVTGLYAQDDLEALLQDLGGQRKKP
                     AAAQPAAPAAAEAPKAEAKPAEPAPAPAPAPAPAPAAEPKAEEKPAPAAEPKAEAKPA
                     EPAPAPAPAPAPAPAAEPKAEEKPAAAPAAEPAPAPAAEPAPAPAAPAVADDADTANL
                     ISELVTLETIRRKSLDSHGFASLEAARKALAAGDYELARDQYKQALEFIGNRPATQAA
                     RDEAKAGVAEAFYLDAKQAMKATDTEKALPLAAQAKEYGHPQAERLIETLKRQPPKAE
                     TDTSSISHRVNEEEYKTRRDEIRRRLRRSRQYYTTAEYDKALEECELILRDYPHEVEA
                     MVLRDRISDRMKAVADVEFEATRSKMIHDVRAAWTPDRYAIDSAQPPSGKGDIKGPEP
                     TVDPLGTKTAEQVIQQKLRDIVIPEVTFRPPATIIDAVDFFKQASRDFDKPEIPLEQR
                     GVNLVLKLSTTPTAAAPAPEGTADPFAPVSVTPAPGGAPTISALSARFISLYDALKLV
                     CDVTGMKFKIRGNIVMIVPANEPDSELMTRSYNVLSSLTDRMTATVSDMGPQGGGDTT
                     FMQMADMGQKQDWKVFFEQLGVKWPDGSSISYLATIGKLRVTNTADQLAVFEQVLEDL
                     NVTPRLIEIEARFVEVAQEDLNSLGFEWLLNSDFS"
     gene            complement(2060..2725)
                     /locus_tag="PK576_09430"
                     /pseudo
     CDS             complement(2060..2725)
                     /locus_tag="PK576_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3137..4117)
                     /locus_tag="PK576_09435"
     CDS             complement(3137..4117)
                     /locus_tag="PK576_09435"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HOE61850.1"
                     /translation="MKRQQITMIVCGLVMAAVCGVAGWFLFTQTTAKNRAAEERDQAY
                     QELQKIYRGKVFPSDENIARVGEDQKVLEGWLVTASNQLHKGDLQIESQSPTSFKQAL
                     QATVRKLSAQAGAVQGKVVAPGFNFGFDQYLGQSDSLPTAEKDGNVNPRLTAQLIIIE
                     KLCEELYAADIMELRSVSREVFDVVKKEEDEQETSSRRRSRRRDDSGGSTRTPDSAAG
                     AKSAYWTSQRFTFEFIARPASLIEALNRLAAMDLFVVVAEAQLRKTSDPLAQFNAKKK
                     ESSAKAAAEGAAAPVDPASLSHVERIVTDPELEPPVEVKLEIDVYSFEGV"
     gene            complement(4114..>5483)
                     /gene="pilM"
                     /locus_tag="PK576_09440"
     CDS             complement(4114..>5483)
                     /gene="pilM"
                     /locus_tag="PK576_09440"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE61851.1"
                     /translation="PFPMSEIVWNHQFIGDATMGEQSAMIVAAKTENVREITDHVVAM
                     GLEPEIVDVAPMALYNCLRYNYSQLDGCTVLLDVGARSTNLIFIEDEKIYNRCIPVAG
                     NAITQEVAKSFQISFAEAEELKRQVAFVSLGGVTASEDEVSERVSKIVRNVVTRLHAE
                     INRSVNFYRSQQGGSAPIRMFLTGGSSVMPHLDTFFRDKLQIEVNYLNPFENVGISNR
                     INSDRVSMDAFVLAESVGLALRRSLACPVEINLMPPEIVKRKSFKKRVPFLALAATGL
                     LLCLFIWAVHAKQLAVLYRAQSAQVTEALGVRKGDQARLNKAQAAEGEVAKRAEALRD
                     VIAQRTAWLKRVDAVRKSLLDGVWLTELVPVKNEGGDVTGLRIVGRGWADKLRAVEEK
                     ERDAGRNTTALEVLRDRLKMQSVFGKGPQDVRITGQKDIEAYLIEFTIEATLAEAAAK
                     DTETK"
BASE COUNT          986 a         1733 c         1608 g         1156 t
ORIGIN      
        1 agctgaaatc gctattcagc aaccactcga agccgagcga gttaaggtct tcctgtgcga
       61 cctccacaaa gcgcgcctcg atctcgatca gacggggcgt gacgttgaga tcttccagta
      121 cctgctcgaa cacggcgagt tggtcggccg tgttggtaac gcgcagcttg ccgatggtag
      181 caaggtaaga gatcgaggaa ccgtccggcc acttcacgcc cagttgctcg aagaagacct
      241 tccagtcctg tttttgcccc atgtccgcca tctgcataaa ggttgtgtcg ccgccgccct
      301 gcggccccat atcgctgacc gtagccgtca tacggtcggt cagagacgag agcacgttgt
      361 aagagcgggt catcagttcg gagtccggct cgttggccgg cacgatcatc acgatgttgc
      421 cgcggatctt gaacttcatg ccggtcacat cgcagaccag cttgagagcg tcataaaggc
      481 tgatgaagcg ggcgctcaac gccgagatgg tcggtgcccc gcccggagcc ggagttacag
      541 agaccggcgc gaaggggtct gcggtgcctt caggagcggg agcggcagcg gtaggggtgg
      601 tgctcagctt gagcacgagg ttcacgccgc gttgctcaag cgggatctcc ggcttgtcga
      661 aatcccggct ggcctgctta aagaagtcca cggcatcaat gatggtggcg ggcgggcgga
      721 aggtgacctc cggaatgaca atatcgcgca gtttctgttg aatgacctgc tcggcggtct
      781 tggtgcccag cgggtcgacg gtcggctcgg ggcccttgat gtcacctttc cctgagggag
      841 gttgagcgct atcgatggca tagcgatccg gcgtccacgc cgcgcgcacg tcatggatca
      901 ttttgctgcg cgtagcttcg aattcgacat ccgcgacggc cttcatacgg tcagagatgc
      961 ggtcacgcaa caccatggct tcaacttcgt ggggatagtc gcgcaggatc agctcacact
     1021 cctccagcgc cttgtcatac tcggcggtgg tgtaatactg gcgcgagcgg cgcagacgac
     1081 ggcggatctc gtcacgccgc gtcttgtact cctcctcgtt gacgcgatgc gaaatgctgg
     1141 aagtatcggt ctcggccttc ggcggctgac gcttcaacgt ttcaatgaga cgctcggcct
     1201 gcgggtggcc atactctttg gcctgagcag ccagcggcaa cgccttctcg gtgtcggtcg
     1261 ccttcatcgc ctgcttggca tccagataga aggcctcggc cacgccggcc ttggcttcat
     1321 cgcgcgcggc ctgagtggcg gggcgattac cgatgaactc gagcgcctgt ttgtattgat
     1381 cccgggccag ttcgtagtcg ccggcggcca gagccttgcg cgcggcctcc agagaagcga
     1441 agccgtgact gtcgagcgac ttgcggcgga tcgtctccaa cgtcaccagt tccgaaatca
     1501 agttggcggt atcagcatcg tccgccacgg ccggagccgc gggcgcggga gcgggttctg
     1561 ccgccggcgc cggcgccggt tccgccgcag gagcagccgc aggcttttcc tcggccttcg
     1621 gctctgccgc aggtgcggga gcgggcgcgg gcgcgggagc aggggccggc tcagcgggct
     1681 tagcctcagc cttcggctcg gccgccggag caggcttttc ctcagccttc ggctctgccg
     1741 caggcgcggg agcgggcgca ggcgcaggcg caggggctgg ctcagcgggc ttagcctcag
     1801 ccttgggcgc ctccgctgcg gctggtgccg caggttgcgc cgccgccggt ttcttgcgct
     1861 gtccacccaa atcctgcaac aacgcttcaa gatcatcctg cgcgtacagg ccggtcacgg
     1921 ccccgcaggc catggccgaa acacttaaca ccaacaacca ccgtttacta tgcgtcatct
     1981 ggagagactc ctaatttgtg gcagcatctt ttccttcaac aggcaataaa gagtactcat
     2041 attagcttgt gtgatggcat taatcaagct tgggaataac tttttgttgc ccgttctttt
     2101 cgttctctat cgtgacagat gtcttctcgc tgtccacggc aaggacgcgg tatgtttctt
     2161 cgcgaacttt gaacgtgccg ccctccaaca ccgagtactc ggcattgtcg agcgaaaaca
     2221 cgatcaccgc ttcaacatct gtgacctttc cgctttcgtt gatcgtcaac gtcattacct
     2281 tgttgtccga cagtcttttc acctctacgg tcgagacgtc cacccgcatt ttaatgccgg
     2341 gcatgttggg attctcgcgc tcgacggatt tcgccgtgat cgttcctgcg gcataaccgg
     2401 tctcgccgat cgtctcgttc tcacgcaccc aataggtcct gcggaccggc tcgacttggt
     2461 taaacacaat ctggtttttg ccatcaggca tcttgttgac accgctaaag atcagcggca
     2521 tgcgtttgcc gcgcaactct ttcacgcgca ggagattgac cagcgccgga tgcgatttgg
     2581 ggtctttcgg gtccgtcttg gcaatgagtt cctcaaggtt cgtaaatccg tcaccgtcga
     2641 ggtcaccttt agcatctgaa ggatccagcg gattcagccc gagcgcgatc tcgatacgat
     2701 ccggaatacc gtcgccatcg gtgtccaagt ccggatcctt gtctggcggc ttgggctgcc
     2761 cccccccgca gaaggggcag gtctcggcgg cgtaggggat gggtttctgg cactccttgg
     2821 tcacacaggt cacacgccgc tccggcgcca gaaagcccgc cttctgagca tcgggagtat
     2881 caagctgcaa cggtgcgcgc gccagacgga tggcggcctc aaactccgcc atatcgatag
     2941 ccgccaacgc ggcagcctcg ggctttagcg agtcgatcct gagcttgtag ttcgcctctt
     3001 cacgctgccg ggcaggccct gccgaacgcg taaggtaaaa cagggagatg acgaggccga
     3061 tcagcaccac gaccacgacc actttatcgt agtacttgac gatggggtta ttcgcgttga
     3121 atttcaaggc cacggtttat actccctcaa acgagtagac atcgatctca agtttcactt
     3181 ccaccggcgg ctccagttcg ggatcagtca cgatccgctc gacatgcgac agcgaggccg
     3241 gatcgacagg tgccgccgcg ccttcggcgg cagccttagc ggaactctct tttttcttgg
     3301 cgttgaactg cgccagggga tcgctcgtct tgcgcagttg ggcttcggcc acgaccacaa
     3361 acaggtccat cgccgccagt cggttcagtg cctcgatgag cgaggctggg cgcgcgatga
     3421 actcaaacgt gaagcgctgg ctggtccaat acgctgattt cgcgcctgcg gcggagtcgg
     3481 gcgtcctggt gctgccaccg ctgtcatccc ggcggcggga acggcggcgc gaggatgtct
     3541 cctgctcatc ttcctctttt ttcacgacat caaagacctc gcgggaaacg ctcctcagct
     3601 ccatgatgtc ggcggcgtaa agctcctcac agagtttctc gataatgatc aactgtgcgg
     3661 tcagacgggg gttcacattc ccgtcttttt ctgccgtcgg caggctgtcg gactggccga
     3721 ggtattggtc gaacccgaaa ttgaatccgg gggcgacgac cttgccctgc accgctccgg
     3781 cctgggcgga gagcttgcgg accgtggctt gaagcgcctg cttgaagctg gtcggggact
     3841 gggactcgat ctggagatcg cctttgtgga gttgattgga ggcggtcacg agccagccct
     3901 ccagcacctt ctggtcctcg ccgacacgcg caatgttctc atcgctgggg aaaactttgc
     3961 cccggtagat cttctgcaac tcctgatacg cttggtcacg ctcttccgcc gccctgtttt
     4021 tagcggtcgt ttgggtaaag aggaaccaac cggcaacccc gcagacggcg gccatcacga
     4081 ggccgcagac gatcatggtg atctgttgac gtttcatttc gtttcggtat ccttcgcggc
     4141 tgcctcggcc aacgtcgcct caattgtgaa ctcaatcaaa tacgcctcga tgtccttctg
     4201 gccggtgatc ctgacgtcct gcggcccttt gccgaacacc gactgcatct tcaggcggtc
     4261 gcgcaatact tccagcgctg tcgtgttgcg cccggcgtca cgctctttct cctcgaccgc
     4321 cctgagcttg tccgcccaac cgcgcccgac aatgcgcagc ccggtcacat cgccgccctc
     4381 attcttgacc ggcaccaatt cggtgagcca gaccccgtcc agcaggcttt tacgcacggc
     4441 atccacgcgc ttcagccacg ccgtccgttg cgcgatcacg tcacgcaacg cctcggcccg
     4501 cttcgcgacc tccccctcag cggcctgcgc tttgttgagc cgggcctgat cgcccttgcg
     4561 cacacccagc gcctcggtca cctgcgcgct ctgggcacgg tatagcaccg caagctgctt
     4621 ggcgtgcacc gcccagatga acaggcagag caacaggcca gtcgccgcca acgcaaggaa
     4681 cggcacgcgc ttcttgaacg acttgcgctt cacgatctcg ggcggcatca gattgatctc
     4741 aaccggacac gccaacgaac ggcgcagcgc caaccccacg ctctcggcca gcacgaaggc
     4801 atccatgctg acgcggtcgc tgttgatccg gttgctgatc ccgacattct cgaacggatt
     4861 gagataattg acctcgatct gcagcttgtc gcggaaaaag gtgtccagat gcggcatgac
     4921 cgacgatccg cccgtcaaaa acatccgaat cggcgcactg ccgccctgct ggctgcggta
     4981 gaagttaacg gagcggttga tttcggcgtg cagacgcgtc accacgttgc gcacgatttt
     5041 cgaaacccgc tcggacacct cgtcctccga ggcggtcacc ccgcccagcg acacgaacgc
     5101 gacctgccgc ttcagctcct cggcctcggc aaaggatatc tggaagctct tggcaacctc
     5161 ttgcgtgatg gcattaccgg ccactgggat gcaccggttg taaatcttct cgtcttcaat
     5221 aaaaatcagg ttggtcgaac gcgcgccgac atcaagcaac accgtgcaac cgtccaactg
     5281 cgagtagttg taacgcaggc aattgtagag cgccatcggc gccacatcga caatctcggg
     5341 ctccagtccc atcgccacga cgtggtcggt gatctcgcgg acattctcgg tcttggccgc
     5401 gacgatcatc gcgctctgct cgcccatcgt ggcgtcgccg atgaactggt ggttccaaac
     5461 gatctcgctc atcgggaacg gga
//
LOCUS       DAOSLE010000165         5390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13603_length_5390_cov_6.761200, whole genome shotgun sequence.
ACCESSION   DAOSLE010000165 DAOSLE010000000
VERSION     DAOSLE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13603_length_5390_cov_6.761200"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PK576_09445"
     CDS             complement(<1..1315)
                     /locus_tag="PK576_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE61852.1"
                     /translation="MIPILKAIAEGRLIELPPCDKNQALRTLAEKLCASADIPAEYNV
                     FENVIRREKQAITYLKYGIACPHARSEEHGGEMACVIGWSPDGIDYGNTDGWPVHLIL
                     MYYIPGSARNAYLTELATLARAIEDDETKHELVNLKDLEDVQSRLESWIATIEGREDA
                     EDDREQVRRATSTVLSQLLMPDIIEMLEDRRFNDLRIFLAAQPTPEIAELIAALQTSD
                     QLLVFRLLPRSMAGEVFSLLEYPSQNLLLENMAQDETRHVLTALSPDDRTALFEELPA
                     NVLQGLLNLLSDKDRRQALSLLSYPKDSVGRLMTNRYVFAREEWTVARTLEQIRAMGN
                     DSETVMMIYIIDERGVLVDELVLRKLILADPDTPISSLMDHQYVALHSLQDREEAVMV
                     FKKYDLHVLPVVDSEGVLLGIVTNDDILDVSEEEATEDIHKGVAIM"
     gene            complement(1414..3744)
                     /gene="ligA"
                     /locus_tag="PK576_09450"
     CDS             complement(1414..3744)
                     /gene="ligA"
                     /locus_tag="PK576_09450"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOE61853.1"
                     /translation="MNRERLEHLRAEIRRHDHLYYVEARPEIGDADYDALYRELEALE
                     RAHPEWVTPDSPTQRVGGAPLDAFRQVRHDPPMMSLDKTHSRGDLTDFDAFLRRQMPD
                     EVWDYVVEPKIDGVAFSLVYARGALTRAATRGNGEVGDDITANIRTLRSIPLVLRDAP
                     SLLEVRGEVYMTREGFAALNRRMEEAGREPFMNPRNAAAGSLKQLDPREVARRPLDAV
                     LYAAVDGSGVEFPTHGGLLARLAEWGFRTPPWQRLCVDIQAVLDAIDELQSLRHAFPF
                     EIDGAVIKVNRRDLYARLGSTAKAPRWARAFKYEPERATTRIGSVTVQVGRTGVLTPV
                     AELTPVLLAGSEIARATLHNADEIARKDIRIGDRVWIVKAGDVIPAVESVQTEERDGT
                     ERVFVMPSVCPECGGAVVRLEGEVAHRCTNPACPAQRVGRLEHFASRDALDIRGIGGK
                     VAEALVAQEWVRDPLDLFDLTLPQLEAFRIGDEQSGTRKFGKNAQTALQSLEAARTLP
                     LDRWLFATGIPSIGVTVAEQIASVHKNFSSLAESPTLQAVLRLDELYAQATTVNPRST
                     ANRPKDEAEREERQNLFNRICGEIGVTGDALVAADAATRTPGSTLPPYYTCKIKPEAA
                     KAVLAFFASEYGARYLQRLKELGIDPCARPKPAAVEGPLAGLTFVLTGTLSQPRRTLA
                     AQIRAAGGTVQDTVTRQTRYLVAGTDAGGVKCDKARALGTEILDEAGLESLLGGTPQN
                     TPPTPPPSATPTPPPGAQAAYRQTELFPSRSVDFRG"
     gene            complement(3764..4750)
                     /locus_tag="PK576_09455"
     CDS             complement(3764..4750)
                     /locus_tag="PK576_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE61854.1"
                     /translation="MNARKRIQPPPRNSPRIGIALGSGGARGWAHLGVLRRLDAFGVR
                     PVCVAGTSIGAIVAALYACNALDTTEALAARLDWKQLAQLFFEVTFPRSGLITGKNFM
                     RLLKDIIPARNIADLPFPYAAVATNLHTEKEVVLSSGNLFDAIRASIGIPGIFTPTRL
                     NGQTLVDGGLVNPLPVSVCRALGAEIVIAVDVNLRTPPLRPRRTPRKPADAPNPHVDA
                     LIASISRLMPDALQTPMSELAHRWTAPRKHNADPLSIFDVLTHTMRLVENQITRHELA
                     LHPPDILIQPEVGNLLTLEFHRGLEGIAAGVRAADEQRSHIEALLNPRPAPS"
     gene            complement(4747..5103)
                     /locus_tag="PK576_09460"
     CDS             complement(4747..5103)
                     /locus_tag="PK576_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61855.1"
                     /translation="MTIRFFAAVLSVTLIMLGGSGCTTSRVIENTRVPEITIEESGTI
                     RFNQEPVEPGKLVSALRSGGIRTSQEVNILIPDQPDRALMRAVSAELLRGGYSRTVFV
                     KNRKATSSFPSARKTK"
     gene            complement(5202..>5390)
                     /locus_tag="PK576_09465"
     CDS             complement(5202..>5390)
                     /locus_tag="PK576_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61856.1"
                     /translation="HPTATQRNICVHYLANAIPLLHRHNQPHTAKELYARLTETLPDS
                     VPKPSFDTVLTGWHPPQQ"
BASE COUNT          943 a         1528 c         1858 g         1061 t
ORIGIN      
        1 gcatgatggc cacacccttg tggatgtctt cggtggcctc ttcttcggag acgtcaagga
       61 tgtcgtcgtt cgtgacgatc cccaacagta caccctccga gtcgacgacc ggaaggacat
      121 gcaggtcgta tttcttgaac accatgaccg cctcttcacg gtcttgcaaa gagtgcagcg
      181 ccacatattg gtggtccatc aaggatgata taggcgtgtc cggatcagcg aggatcagtt
      241 tgcgcagcac cagttcgtct accaaaacgc cgcgctcgtc aatgatgtaa atcatcatga
      301 cggtttccga atcattgccc atggcgcgga tttgttccag cgttcttgcg acggtccact
      361 cttcacgggc gaagacatag cggttggtca tcaaacgccc aacactgtcc ttcgggtagc
      421 tgagcagact gagggcctgt ctgcggtctt tgtcgctcaa cagattcagc agcccctgta
      481 acacgttggc cggtaactcc tcgaacagcg cggtccggtc gtccggcgaa agtgctgtga
      541 gaacatgacg cgtctcgtcc tgcgccatat tttccagcaa caggttctgg gaggggtatt
      601 cgagcaggga gaagacctca ccggccatgc ttcgcggcaa caggcggaac accagcaact
      661 ggtcggatgt ctgaagcgcc gcgatcaact ccgcgatttc cggggtgggc tgcgcagcga
      721 ggaaaatgcg caggtcgtta aaccgccgat cctccaacat ctcgatgatg tcgggcatca
      781 gcagttggct gaggactgtg ctggtggcgc ggcgcacctg ctcgcggtca tcctctgcgt
      841 cctcacgtcc ctcaattgtc gcgatccacg attcaagacg ggactggacg tcctccagat
      901 ctttgaggtt aaccaattcg tgcttggtct cgtcgtcctc gatagcccgg gcaagcgtcg
      961 ccaattcagt cagataggcg ttacgggcag agccggggat gtagtacatc aggatcagat
     1021 gcacaggcca tccgtcagtg ttaccgtaat caatgccgtc agggctccag cctataacgc
     1081 aagccatctc gccgccatgc tcttccgagc gggcgtgcgg acaggcgata ccatatttga
     1141 ggtaggttat ggcctgcttt tcacgccgaa tgacattttc gaaaacgttg tactccgccg
     1201 gtatgtcggc agaggcacaa agtttttccg caagcgtgcg gagtgcctga tttttgtcgc
     1261 agggcggcag ctcgatcaac cgtccttcag cgatagcttt gagaattgga atcactgctt
     1321 ttgaccggta aaaaggatga agggtagact gaaaacaaac gggttcccac ttccacgatt
     1381 ggcagtcatt atgcgaaagg gaagggaaga gagtcaaccc cgaaagtcaa cggatcgcga
     1441 cgggaacagt tcggtttggc ggtatgcggc ttgggcaccg ggtggcgggg taggcgtcgc
     1501 gctgggcggc ggggtggggg gcgtgttttg cggtgtgccg ccgagcagac tttccagtcc
     1561 ggcctcgtcc aggatttccg tgccgagcgc acgcgccttg tcgcatttca cgcctcccgc
     1621 gtctgttccc gccaccagat agcgggtctg ccgggtgacg gtgtcctgca ccgtaccgcc
     1681 cgcggcccgg atctgggcgg caagggtgcg acgcggctgc gagagcgttc cggtcaggac
     1741 gaaggtaagc ccggcaagcg gtccctccac ggcggccggt ttgggccggg cgcacgggtc
     1801 aatgccgagt tccttcaagc gctggaggta gcgcgcgccg tattcggagg caaagaaggc
     1861 gaggaccgcc ttggcggctt cgggtttgat cttgcaggtg tagtatggcg gcagggtgct
     1921 gccgggcgtc cgggtcgcgg cgtccgccgc gaccagcgcg tcacccgtca cgccgatctc
     1981 accgcagatg cgattgaaga ggttctgccg ctcttcgcgt tcggcctcgt ccttgggccg
     2041 gttggcggtc gagcgcgggt tgacggttgt ggcttgggca taaagctcgt ccaatctgag
     2101 cacggcttga agggttggtg attccgcaag gctggagaag ttcttatgca cggatgcgat
     2161 ctgttctgcc acggtcacac cgatgctggg gatgccggtc gcaaagagcc aacggtccaa
     2221 cggcagggtg cgggctgctt ccagagattg tagcgcggtc tgcgcgttct tgccgaattt
     2281 gcgcgtgccg ctctgctcat cgccgatgcg gaaggcctcc agttgcggca gggtcaggtc
     2341 gaagagatcg agcggatcgc gtacccattc ttgcgcgacc agggcttcgg cgaccttgcc
     2401 gccgatgccg cggatgtcga gggcgtcacg cgaggcgaaa tgttcgaggc ggccgacgcg
     2461 ctgcgcggga caggcgggat tggtgcagcg gtgggcaact tcgccttcca gacgcacgac
     2521 cgcgccgccg cattcgggac agacggacgg catgacgaag acgcgctccg tgccgtcgcg
     2581 ctcttcggtc tgcacgctct ccaccgccgg gatgacatcc ccggccttga cgatccagac
     2641 gcggtctccg atgcggatgt ctttacgggc gatctcatcc gcgttgtgca gggtggcgcg
     2701 ggcgatttcc gaaccggcga gcaatacggg cgtgagttcc gccaccggtg tgagcacacc
     2761 ggtgcggccg acctgcacgg tgacggatcc gatgcgcgtg gtggcgcgtt ccggttcgta
     2821 tttgaaggcc cgcgcccagc gcggggcttt ggcggtgctg ccgagccggg cgtacagatc
     2881 gcggcggttg accttgatga ccgcgccgtc gatctcgaac gggaacgcgt gccgcaggct
     2941 ttgcagttcg tcgatagcgt cgagaaccgc ctggatatcc acacagagcc gttgccaagg
     3001 cggggtgcgg aaaccccatt ccgcgaggcg cgcgagcaga ccgccgtggg tcgggaactc
     3061 cacgccgcta ccgtcgaccg ccgcataaag cacggcgtcg agcggacggc gcgcgacttc
     3121 gcgggggtcg agctgtttga gtgatccggc tgcggcgttc cgggggttca tgaagggctc
     3181 gcgacctgcc tcctccatgc ggcggttgag cgcggcgaac ccctcgcgcg tcatgtagac
     3241 ctccccgcgc acttcaagca gcgagggggc gtcccggagg acgagcggga tcgagcggag
     3301 ggtccggata ttcgcggtga tgtcatcgcc cacctcgccg ttaccgcgcg tggcggcacg
     3361 cgtcagggca ccgcgggcat acaccagcga aaaggccacg ccgtcgattt tgggttccac
     3421 cacgtaatcc cagacctcat cgggcatctg gcggcgcagg aaggcgtcga aatcggtcag
     3481 gtcgccgcgc gagtgcgttt tgtcgaggct catcatcggc ggatcgtggc ggacctgccg
     3541 gaaggcgtcg aggggggcac cgcccacgcg ttgggtcggg gagtcggggg tgacccattc
     3601 ggggtgcgcg cgttcgagcg cttcgagttc gcggtacaac gcatcgtaat ccgcatcacc
     3661 gatttcaggg cgcgcctcca catagtagag gtggtcgtgc cgccggattt cggcacggag
     3721 gtgttccagg cgttcgcgat tcatgttacg gtctccgggg ttgttacgac ggggcgggac
     3781 gcggattgag cagtgcttcg atgtgggatc gctgctcatc cgccgcgcgg accccggcgg
     3841 cgatcccttc gagcccgcgg tggaattcga gggtgagcag gttgccgacc tcgggctgaa
     3901 tcaggatgtc gggcgggtgg agcgcgagtt cgtgccgcgt gatctggttc tccacaagac
     3961 gcatggtgtg ggtgagcacg tcaaaaatcg agagcgggtc tgcgttgtgt ttgcgcgggg
     4021 cggtccagcg gtgcgccaat tcggacatgg gcgtctgcag ggcgtccggc atgagacgcg
     4081 aaatcgaggc gatcagcgcg tcgacatgcg ggttcggggc gtcggcgggt ttacgtggcg
     4141 tgcggcgcgg ccggaggggc ggggtacgga gattgacatc caccgcgatc acgatctcgg
     4201 cccccagggc tcggcagacc gagaccggaa ggggattgac gagcccgccg tccaccaggg
     4261 tttggccgtt gagccgcgtg ggtgtgaaga tgccggggat gccgatactg gcgcggatcg
     4321 cgtcgaacag attgccggag gagagaacca cctccttttc ggtgtggagg tttgtggcga
     4381 ccgccgcata cgggaaaggc agatcggcga tattccgcgc ggggatgatg tctttgagga
     4441 gccgcatgaa gtttttgccg gtgatcaggc ctgagcgcgg gaacgtgact tcaaagaaca
     4501 gttgcgcgag ctgtttccaa tccaggcggg cagcgagcgc ctccgtggtg tcgagcgcgt
     4561 tgcaggcgta aagcgcggcg acgatggcgc cgatgctggt ccctgccaca cagacgggcc
     4621 gcaccccgaa tgcgtccagt cggcgcagca ccccgagatg agcccagccg cgcgcaccgc
     4681 cgctgccgag cgcgattccg atacgagggg agttgcgggg agggggttgt atgcgcttcc
     4741 gtgcgttcat ttcgttttcc gggctgaggg gaaggaggag gtggctttgc ggttcttcac
     4801 aaaaaccgtg cgggagtaac cgccgcgcag caactcggcg gacaccgcgc gcatgagcgc
     4861 ccggtccggt tggtccggaa tcaggatgtt cacctcttgg ctggtacgga tgccgccgga
     4921 acgcaaggct gaaacaagtt tgccgggttc gaccggttct tggttgaacc ggatcgtgcc
     4981 ggactcctcg atggtgattt ccggtacgcg ggtgttttca atgactctcg atgtggtgca
     5041 gccggatccg ccgagcataa tgagggtcac gctgagcacg gccgcaaaaa agcgtatcgt
     5101 catcatctct cctgacttgg tttcccgctg ttgcgggtat ggatgacaaa aattttacca
     5161 cagaaagccg atttgcggta aacaaaggat tccgcgacgg gttattgctg gggggggtgc
     5221 cagccggtga gcaccgtgtc aaatgagggt ttcggtacgc tgtccggaag cgtttcggtc
     5281 agacgcgcgt agagttcttt agcggtatgg ggctggttgt ggcggtgcag cagtgggatc
     5341 gcgttggcca gataatggac gcagatgttt cgctgtgtcg cggtggggtg
//
LOCUS       DAOSLE010000166         5373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13648_length_5373_cov_9.647236, whole genome shotgun sequence.
ACCESSION   DAOSLE010000166 DAOSLE010000000
VERSION     DAOSLE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13648_length_5373_cov_9.647236"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..3556)
                     /locus_tag="PK576_09470"
     CDS             complement(89..3556)
                     /locus_tag="PK576_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61857.1"
                     /translation="MRRLMGMGALAVMVAGGAYGATPPSAASDETGRLRLYAFGDLIG
                     NAVSNAAGPGNAMLYKAASGAVPVTGPGRRAGSRAVVLDADVFSAPAVTQASNAFTVA
                     LWLRPQAAGSKKAGSRSNGMIASNGSGYYDGWRLLLHDWRTRCPTIELGRGKDKGALG
                     VHARDSLAVGFWNHLAATWDGARVRLYVNGMLSAETPYAGPLVPPRGALCAGFSGYGV
                     GSLGMAIDTLAVYDRALPPETVARIALDGEALPADCVALLGQAQAAAGAGARSEASQA
                     YRALAGKGEAPAIWRQWAALAALRFIETAADRRAGVEACAAFYSDASVPETLRGQAIE
                     FLAQICRRGLGGEVLPSRILADLPEHLELDAGDPRLFGLALAEAYQRERQFDAAYGVY
                     RQLLTAAGDEAEAAAGIRQGYARALWQGGCREEARAQYAALAADTRLPESARGLAALA
                     VAQVWREEGKYAEAAAAYRAVTGTVSRFTHLLDEARVGAEACANLLEGKPARDPEASR
                     QRLAPLPEPAVRFVVSPKGRDTNPGTFEQPFATLERARDAVRAAKRGGSLPPGGACVF
                     LRGGRYAVTNTFVLEKGDSGSFGAPVVYCAWPGERPVFDGGFRVRGFWRVRDAGTLKR
                     LPEEARGHVHVANLTKQGYTALEAQASYGRAQTNETVRELFQDGQPLEIARWPNDGFL
                     KMATANITNLTFSCPTNRLARWTQADDLMANGYWFHLWAECTVPLAAVDAATGVFTFR
                     QKPDHNLRAGYPFYVLNLLEEIDRPGEWFLDRAAGRLYVWPLKQPWRSTLTLSQWNRP
                     FVEARKVQEVVFRGLTFEYGRQNGMVLDACANVTVAGNVIRRLGGTALIVTQGANIRI
                     YGNRMETLGHAGMRVEGGNRTNLTSGRIVIENNEVSHFARCSRTYNPALLLDGCGARV
                     AHNHFHHAPSSAMRIEGNDHLIEFNRVEHMVRESDDQGGIDMWCNPSYRGVVIRYNLW
                     RDIGGGHAPCGQAGIRFDDAISGMVVYGNRFERTSTGHFGGVQIHGGQDNIIDNNLFT
                     ACRYGVSFSAWGQKRWEDFLGRERIRNLMYSDVNITLPPYSKRYPELAGLPGKADVNS
                     IWRNLFIGTEEALHKKPKGTETWENRVFAEAPDLEEVAACSPFETLPPEDEIGLYADP
                     FRAAASRDF"
     gene            complement(3699..4235)
                     /locus_tag="PK576_09475"
     CDS             complement(3699..4235)
                     /locus_tag="PK576_09475"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOE61858.1"
                     /translation="MKKRKLPLSKVYTLIEPGPVVLVTTMDQGRPNVMTLAWHTMLDF
                     EPPLLGMVLSEQNHSFAVLRKTKACVINLPSVALRKAVVGCGNTSGRDTDKFAAFGLT
                     PRPAKQVAAPLIDECFASLECVVTDTRMVRQYNLFVLEVVQAWIDPDCGQPKTLHHCG
                     TGNFMVAGRRITLPSAMK"
     gene            complement(4364..5116)
                     /locus_tag="PK576_09480"
     CDS             complement(4364..5116)
                     /locus_tag="PK576_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOE61859.1"
                     /translation="MRYRKQILAPCFGEATQHCLAKATVAIVGCGGLGTWQAELLARM
                     GIGRLRLADDDRVTLENLHRQILFTEQDAVAGLLKVRAAAARLRQINTHVVIEPYEMR
                     ITRDTLDAFAAEADLVLDATDHAPTRFLINDFCLAHAIPWIYTGVAGEEGLILPVLPS
                     GGPCLRCLYPDPPEPDETANCRTDGILPMTVAMAVSLQIAQAVRVLNGNAEPGTLIRF
                     NVWSAVARRVRLERLPTCPGCGLRLRNGTNET"
BASE COUNT          985 a         1872 c         1581 g          935 t
ORIGIN      
        1 cacagcctca cagcctttga gattgcgggc tccgcccgca tctcaatacg gtgcgtcggg
       61 cgcaccgcaa aacccaatgt ttaccgtcct agaaatcccg tgatgcggcc gcacggaagg
      121 gatccgcata caggccgatc tcgtcttcgg gcggcagcgt ctcgaaaggt gaacaggccg
      181 cgacctcttc caaatcgggg gcttcggcga acacgcggtt ctcccaggtc tcggttccct
      241 taggcttttt gtgcagcgcc tcttcggtcc cgatgaaaag gttgcgccag atgctgttga
      301 catccgcttt gccgggcagc ccggccagtt cgggataccg tttgctatag ggcggcagcg
      361 tgatgttgac gtctgaatac atcaggttcc ggattctctc gcggcccaga aagtcctccc
      421 agcgcttctg gccccatgcc gagaaactca ccccgtaccg gcacgcggtg aagaggttgt
      481 tgtcgatgat gttatcctgc ccgccgtgga tctgcacgcc gccgaaatgc cccgtcgaag
      541 tccgctcgaa gcggttgccg taaacgacca tgccgctgat cgcatcgtca aaccggatgc
      601 ccgcctgacc gcacggcgca tggcctccgc cgatgtcccg ccacagattg taacggatca
      661 ccacgccgcg gtaggacgga ttgcaccaca tgtcgatccc gccctgatcg tccgactccc
      721 gcaccatatg ctccacgcgg ttgaactcga tcaggtggtc gttgccctcg atccgcatgg
      781 cgctggacgg cgcgtggtga aaatgattgt gcgccacgcg cgcgccgcag ccgtcgagca
      841 gcagcgcagg gttgtaggta cgcgaacagc gggcgaaatg ggagacttcg ttgttttcga
      901 tcacgatgcg tcctgaggtc aggttggtac ggttaccgcc ctcgacccgc atgcccgcat
      961 gcccgagcgt ctccatccgg tttccgtaaa tccggatgtt cgcgccttgg gtgacgatca
     1021 gcgccgtgcc gcccaaacgc cggatcacat tccccgccac ggtcacgttg gcgcaggcgt
     1081 ccagcaccat cccgttctgc cgcccgtact cgaaggtcag gccgcggaac accacctcct
     1141 gcaccttgcg ggcctcgaca aacggacggt tccattgcga cagcgtcagc gtgctgcgcc
     1201 aaggttgttt gagcggccac acgtagagcc gtcccgcggc ccggtcgaga aaccactcgc
     1261 cgggccggtc gatttcctcc agcaggttca gcacgtaaaa gggatagccc gcacgcaggt
     1321 tgtgatccgg cttctggcga aacgtaaaga ctcctgtcgc ggcatcgacc gcagccaagg
     1381 gcaccgtgca ctcggcccac aggtggaacc agtagccgtt cgccatcagg tcatccgcct
     1441 gcgtccagcg cgccaaccgg ttggtcggac aggagaaagt caggttggtg atgttggcgg
     1501 tcgccatctt gaggaagcca tcgttgggcc accgggcgat ctccagcggc tggccgtcct
     1561 gaaacagctc gcgaaccgtt tcgttggtct gagccctccc gtacgaggcc tgcgcctcca
     1621 acgcggtata cccttgtttc gtcaggttcg cgacgtgaac atggccgcgc gcctcctcgg
     1681 gcagccgctt caacgtgccg gcatcgcgca cgcgccagaa tccccgcacg cggaacccgc
     1741 cgtcgaacac cggccgttcg ccgggccagg cgcaatacac taccggcgct ccaaaactgc
     1801 cggaatcccc tttttcgagg acgaatgtgt tggtcaccgc atagcggccg ccgcgcagaa
     1861 acacgcaggc gccgcccggc ggcagactgc ccccccgttt ggcggcgcgc accgcgtcgc
     1921 gcgcgcgttc cagcgtggcg aacggctgct cgaaggtgcc ggggttcgtg tcccggcctt
     1981 tgggcgacac aacgaaacgc accgccggtt cgggcagcgg ggcgagacgc tgacggctgg
     2041 cctcgggatc gcgcgcaggc ttaccctcca gcaggttagc gcacgcctcc gcgccaacac
     2101 gcgcctcatc cagcagatgc gtaaaccggc tgactgtccc tgtcaccgcg cggtatgccg
     2161 cggcggcttc ggcgtacttg ccctcctcgc gccatacctg cgccaccgcc agcgccgcga
     2221 gtccgcgcgc gctttccggc agacgcgtat ccgccgccag cgcggcatac tgtgcgcgcg
     2281 cctcctcccg gcaaccgccc tgccagagtg ctcgggcata accctgccgg attccggcag
     2341 ccgcctccgc ctcatccccc gccgcagtga ggagttgccg gtacacaccg tacgccgcat
     2401 caaactgccg ctcgcgctga taggcctccg caagggcgag accgaacagg cgcgggtccc
     2461 cggcatccag ttcgagatgc tccggcagat cggccagaat ccggctgggg agcacctcgc
     2521 cacccagtcc tctgcgacag atctgcgcca gaaattcaat cgcctgaccc cgcagggttt
     2581 caggcacgga cgcgtcggag taaaacgccg cgcacgcctc cacccccgca cggcgatcag
     2641 ccgccgtttc aatgaaccgt agcgcggcga gtgcggccca ttgccgccag atggctggcg
     2701 cctcaccctt cccggccaac gcccgatacg cctgcgacgc ctcgctcctt gcgcccgcgc
     2761 ctgccgccgc ctgcgcctga cccagcaacg ccacgcagtc ggcgggcagc gcctcaccgt
     2821 ccagcgcgat ccgggcgacc gtctcgggcg gcagtgcgcg gtcatacacc gcaagcgtgt
     2881 cgatcgccat gccgagcgag ccgaccccgt aaccggagaa ccccgcgcag agcgcgccac
     2941 ggggcggaac gagcgggccc gcatacggcg tttccgccga caacatcccg ttgacataca
     3001 ggcgcacacg cgcaccatcc caagtcgccg ccagatgatt ccagaagcct acggccaatg
     3061 agtcccgcgc gtgcacgccc aacgcgccct tatcttttcc ccttcccaat tcgatcgtcg
     3121 ggcaacgcgt ccgccaatcg tgaaggagca gacgccaccc gtcataataa ccgctgccat
     3181 tcgaggcgat catcccgttc gagcggcttc ccgctttttt cgagcccgcc gcctgcggcc
     3241 gcaaccaaag cgccaccgtg aacgcgttcg atgcctgcgt cacagccggg gcagagaaga
     3301 catccgcgtc gagcaccact gcccgcgaac cggcccgtcg tcccggcccc gtgaccggta
     3361 ccgcgcccga cgccgcttta tacagcatgg cgttacccgg ccccgccgca ttggagaccg
     3421 cattaccgat caggtctccg aacgcgtaca gccgtaaccg tcccgtttca tcgcttgcgg
     3481 cagacggcgg cgttgcccca tatgcgccgc ccgcaaccat gaccgccaac gcccccatcc
     3541 ccatcaatcg tctcacctgc ttattcatgc ctccagtgta aacgacccgt cccggatgtt
     3601 caagacgggc tcgttcccga tgccgcaaaa gattctttat gcgcttggtg tcagattggg
     3661 ttgcggtgcg gactgtaccc gcattcgtga gccccggatc acttcatcgc cgacggcagc
     3721 gtgatgcgcc gcccggccac cataaagttt cccgtaccgc agtggtgcag tgttttcggc
     3781 tggccgcagt ccggatcaat ccacgcctgc acgacctcca gcacaaacag gttatactgc
     3841 cgcaccatac gggtgtcggt gaccacacat tcgagcgaag cgaagcactc gtcgatcaat
     3901 ggggccgcga cctgtttcgc cggacgcggc gtgaggccga acgccgcgaa tttatccgtg
     3961 tcgcgacccg aggtgttgcc gcatccaacc accgccttgc gcagagcgac tgagggaaga
     4021 ttgatcacac aggccttggt cttacgcaat acggcgaacg agtggttctg ttcgctgagc
     4081 accatgccga gcagcggcgg ctcgaaatcc agcatcgtgt gccaagcaag ggtcatcaca
     4141 ttcggacgcc cttgatccat cgtcgtcacc agcacaaccg gcccgggctc aatgagggtg
     4201 tagactttgg agagcggcaa ctttcttttt ttcatgtccc cagtgtaaac gacccgtccc
     4261 ggatgtgcaa ggttgggaaa aaaggaagag cgttcaacgt ccaagggtcg aatcgaaaat
     4321 cttaacacgc ggagtgaaaa aatcagcgga tcggtcagaa acgtcacgtc tcgttcgtcc
     4381 cgttccgcag gcggaggccg catccgggac aggtgggcag gcgttcgagc cggacacgcc
     4441 gtgccaccgc agaccagaca ttgaaccgga tgagcgtgcc gggctccgcg tttccgttca
     4501 acacgcgcac cgcctgggcg atctgcaggg agaccgccat ggcaacggtc atgggcagaa
     4561 tgccgtccgt gcggcagttg gcggtctcat caggttcagg cggatcggga tagaggcagc
     4621 gcagacaggg cccgccggac ggcagcaccg gcaggatcag cccttcctcg cccgccacac
     4681 ccgtgtaaat ccatgggatc gcgtgggcca ggcagaaatc attgatcaga aagcgcgtgg
     4741 gcgcatgatc cgtggcatcc agcaccagat cggcctccgc ggcaaaggca tcgagcgtgt
     4801 cgcgcgtgat ccgcatctca tatggttcga tcaccacatg ggtgttgatc tgccgcaagc
     4861 gcgcggcagc ggcgcgaact ttcaggagac ccgccaccgc atcctgctcc gtgaacagga
     4921 tctgccggtg caggttttcg agcgtcacgc gatcgtcatc cgccagacgc aaccgtccga
     4981 tgcccatgcg cgccagcaac tccgcctgcc aagtgccgag tccaccgcat cccacaatgg
     5041 ctacggtggc ctttgcgaga cagtgctggg tcgcctctcc gaaacacggc gccaagatct
     5101 gtttccggta tcgcatcttc ccctccccca tgccgcgtct ccggcgcgtc cgggtgatcc
     5161 ggccgcccgc gtgtccgcat cacgggaaag cataccaacg aaacacgccg tcgtccaccc
     5221 cgtcacgaac cgagagcgtg tctctaccgt agcccttaaa agcgcgttga aaattcaact
     5281 ctcttgggtt tcagggtttc atggtttcgg ggtttcaggg tttcagggtt tcatccaaat
     5341 cgggatcggg atcgctatcg ggatcgggat cga
//
LOCUS       DAOSLE010000167         5368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13666_length_5368_cov_8.519669, whole genome shotgun sequence.
ACCESSION   DAOSLE010000167 DAOSLE010000000
VERSION     DAOSLE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13666_length_5368_cov_8.519669"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..806)
                     /gene="deoC"
                     /locus_tag="PK576_09485"
     CDS             complement(114..806)
                     /gene="deoC"
                     /locus_tag="PK576_09485"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOE61860.1"
                     /translation="MRVEPVTQTTMARMMDHAVLNPAMTENDIRRAAAMCRARGVGNL
                     CVRPTDAALAASLLKGSATTVAVVVGFPHGASRSEVKALEARLAIEDGAAELDMVMNI
                     GKFLSGDYEYVKRDIEAVVAVAKPQGVLVKVIQESCLLTLDQVAKACELTIAAGADFV
                     KTSTGFNGDGATVEQVEVMLKTCAGRTKVKPSGGIRDWERAAMFVRMGVDRLGVSSTD
                     KILDGAPTEDGC"
     gene            complement(953..2080)
                     /locus_tag="PK576_09490"
     CDS             complement(953..2080)
                     /locus_tag="PK576_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61861.1"
                     /translation="MRINVTTYLLAGLFVTAAGGAMADEACAPCETYRPKLFKRFGDH
                     VNTPDGLAQDRHGNIFLAAPNFVDKSYPGAILKMDKRSGKWSIFVTGLVHPETGHGAP
                     MGMEFGEDGNLYYCDNQYFSNKNYKSRVMRVVIDKAGEPVKIEPVVENIKLANALRVR
                     GNAIFFTDTFFDVAGKNLGGVYRVPISAFRDRPVRLLPKEQAANDPYCLGTTETKPLE
                     HRKDAAGADGLCIDKDGNLYTGNFGDGHFYILRLKPDGTYEKPATLFYDPKVFPCCDG
                     ICYYAKKNWIILTDSERNAVRYWDIKAGTMGLLWENEDTDGADGLLDQPCEPMVWGDK
                     LLVVNFDMSFPGLKNTRNDEVHTLSVIDLKEGGCPFLNLFR"
     gene            2534..4165
                     /locus_tag="PK576_09495"
     CDS             2534..4165
                     /locus_tag="PK576_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOE61862.1"
                     /translation="MTTQRKMSILASVLCAGVVQTGWTATSAYWTFQDQEPGVLATTL
                     VSEVHSDVLVGQGKSNGSGRLPSHDADVPATKIYDGLFGPLLNSNNTASLYFTNAVAY
                     PAQTNSQNGGYVEVADVPSLLCPTNFTIEFFVKIARDVDWPLLVGKARERGASWAIDI
                     DRNNSLKLRMDTQPIDQPGTTNGFNENLLGSNIQDGKWHHVAVTYSASKKTATFYVDY
                     EYVSSKASYAHEVTYNTNNYPLLMGRGAGSYQAFDGWLDEVRLSDVVLAPHQFLRTYA
                     VPEDAQLYYTFDDAATVPSTATTLVCEAHSPAMDGLTADTRPTFSPDIPPASTTRITQ
                     GYKGTVVNADNSSSLFFVNTGLPGNTDSAYGGQVAIPGFLHPSNFTVEVFFKMNRHVN
                     YPLLVGKVRTGSSPTWSLSLSGADDLVRCRFDTYPVDWNLGEHTYAENSQNGFNQCFS
                     TGVPGNDGKWHHAAFSYETATKNVKIYVDYVLKANGTTINPIVYTDGDILIGMGAGER
                     AFDGWIDEVRITPRLLTPDEFLYTQPRLGTLITLD"
     gene            4385..4975
                     /locus_tag="PK576_09500"
     CDS             4385..4975
                     /locus_tag="PK576_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOE61863.1"
                     /translation="MREGTTRRAFLVQAGVAGTALAGAAALTAKAGNQTTEARNMAES
                     KKILVAADPYAVSLKDAVVAHLKGQGYEVTDFSTRDGKACPYFESAVAVCRAMQAGQA
                     PRAILFCGTGMGMSIVANRFKGCTAAVVESVYAAKMCRAINNANVLCLGQMIWGPDMA
                     KVAVDAFLSTRFTEGLEGIADFLRDACGKVEAIRPQ"
     gene            complement(5040..>5368)
                     /locus_tag="PK576_09505"
     CDS             complement(5040..>5368)
                     /locus_tag="PK576_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61864.1"
                     /translation="VSGFKVSGFQGFIQIGIGIGIGIDYLTTKHSLGFQGFRVSGFWF
                     QGFIQIGIGIAIAIGIDCLTTASQSHSLTASQASQPLRLRAPPASQYVSPAMETPTLA
                     PSVQSA"
BASE COUNT         1087 a         1604 c         1604 g         1073 t
ORIGIN      
        1 cgattccgat agcgataccg accccgatac cgatttggat gaaaccctga aaccctgaaa
       61 ccaaagagag ttgaacgttc aactgttctt ttaggttccg cgtttgtctt atcttagcac
      121 ccgtcttccg ttggagcgcc atccagaatc ttgtcggtcg agctgacgcc caggcgatcc
      181 acccccatgc ggacaaacat cgcggcgcgt tcccagtcgc ggatcccgcc cgagggtttg
      241 actttggtgc ggcccgcgca ggtcttcagc atgacttcga cctgctcgac cgttgcgccg
      301 tcgccgttga acccggtcga ggtcttcacg aaatcggctc cggcggcgat ggtgagttcg
      361 caggctttcg cgacctgatc aagggtcagc aggcatgact cttgaatgac ctttaccagg
      421 acgccttggg gtttggccac cgccacgacc gcttcgatat cccgtttcac gtactcgtag
      481 tcacccgaga ggaacttgcc gatgttcatc accatgtcca actcggccgc gccgtcctcg
      541 atggccagcc gggcttcgag cgccttcact tcggaacggc tcgccccgtg cgggaaaccg
      601 accaccaccg cgacggttgt ggctgaacct ttgagcaggg atgccgcaag cgccgcatcg
      661 gtggggcgca cgcagaggtt gccgacgccc cgggccctgc acatcgcggc ggcccggcgg
      721 atgtcgttct ccgtcatggc ggggttcaac accgcatggt ccatcattct ggccattgtt
      781 gtttgggtga cgggttcaac gcgcatgggg ggctcctcgg acctcatttc ccggaggcca
      841 aacggctaaa ataagatggg gcagtttccc cgaaaccgcc gcggacggtt cggggaactg
      901 tcctcacggg caagcagacg cttacccctc ccgttcccgc acgggcaaca catcacctga
      961 acaggttcag gaaggggcag ccgccctctt tcaggtcgat cacgctaagg gtgtggacct
     1021 cgtcgttccg ggtgtttttg aggcccggga aggacatgtc gaagttcacg accagcagct
     1081 tgtcgcccca caccatcggc tcgcacggct gatccagcag accgtccgcg ccgtcggtgt
     1141 cctcgttctc ccacagcagt cccattgtcc cggccttgat gtcccagtag cgtaccgcat
     1201 tgcgctcgga gtccgtcagg atgatccagt tcttcttcgc gtagtaacag atgccgtcac
     1261 agcacgggaa caccttcggg tcgtaaaaca gcgtggccgg cttctcgtac gtgccgtccg
     1321 gcttcagcct gaggatatag aagtggccgt cgccgaagtt gccggtgtag aggttgccgt
     1381 ccttatcgat gcacagacca tccgcgccag cagcgtcctt gcggtgctcc agcggcttgg
     1441 tctcggtggt gccgaggcag taggggtcgt tcgcggcctg ctctttgggc agcagtcgga
     1501 ccggacggtc gcggaacgcg ctgatcggca cgcggtacac accgcccaga tttttgccgg
     1561 ccacgtcaaa gaaggtgtcc gtgaaaaaaa tcgcgttccc ccgcacgcgc agggcgttgg
     1621 cgagtttgat gttttcgacc accggctcga tcttgaccgg ctcgcccgct ttgtcgatca
     1681 ccacgcgcat cacgcgcgat ttgtagttct tgttgctgaa atactggttg tcgcagtagt
     1741 agaggttgcc gtcctcaccg aactccatgc ccatgggcgc gccgtgtccg gtttcgggat
     1801 ggacgagacc cgtcacgaaa atcgaccact tgccggaacg cttgtccatc ttcaggatcg
     1861 cgccgggata ggatttgtcc acgaaattgg gcgccgccaa gaagatgttg ccgtgccggt
     1921 cctgagccaa cccgtccggt gtgttgacgt ggtcgccgaa ccgtttgaac agctttggcc
     1981 ggtacgtttc gcacggggcg caggcctcat ccgccatggc gccgcctgcg gccgttacga
     2041 acaagcctgc cagcagatac gttgtcacgt tgattctcat cgcatatccc tttccttgtg
     2101 ccgatggctg cggcggacct ccgcctgcca tccggattaa acgtatcgaa atcttaccaa
     2161 gcctctccgg gaatggcaat cacctcaaaa aacgtgtcac ggcatagggg gttccagagg
     2221 aacgcgcatg accggaaagc ggaatcagaa acagcagtcc cgtgctggtt tcgggttcct
     2281 ggtcttgggt ttaccggtac cgcgtgtatg tcgacttgcg gcctgcggca tttgacctgc
     2341 ggcgggggag gcctctgatc cgactgaacc cagaaaccaa acccttcagc ggttcgggaa
     2401 acaggaatgc gccccgatag agaaaaagat caaaatcggg attgattttt ccggtgcttt
     2461 atcgcatatt ttcatcgtaa tcatgacatg cgggtgtgtc ccacggatgt ggctataaaa
     2521 aaaggaaaca accatgacga ctcaacgaaa gatgtcgatt ctcgcgtcgg tgttatgtgc
     2581 cggagttgtt cagaccggat ggaccgccac gtcggcgtat tggacctttc aggatcagga
     2641 gccgggcgtt ttggcgacca cgctggtcag tgaggtccac agcgacgtgc ttgtcggaca
     2701 gggtaagtcc aacggctcag gccggcttcc ctctcatgat gcggacgtgc cggcgacaaa
     2761 aatctacgac ggacttttcg gcccgctgct caacagcaac aacaccgcct cgctctattt
     2821 caccaacgcg gtggcatatc cggcccagac caattcgcag aacggcggtt acgtcgaggt
     2881 cgcggacgtg ccctccctgc tctgccccac caacttcacg atcgagtttt tcgtgaaaat
     2941 cgcccgcgac gtggactggc cgcttctggt cggcaaggca cgcgagcgcg gcgcaagctg
     3001 ggcgatcgac atagatcgaa acaattcgct gaaattgcgc atggatacgc aaccgatcga
     3061 tcagccggga acgaccaacg ggttcaacga aaacctgctc ggctcgaata tccaagacgg
     3121 caaatggcat catgtcgccg tcacctacag cgcatcgaag aagaccgcga cgttttatgt
     3181 ggattatgag tatgtgtcct ccaaggcgag ttacgcccac gaagtcacgt ataacacgaa
     3241 caactatccc cttctgatgg gaaggggcgc ggggagttat caggccttcg acggctggct
     3301 cgacgaggtg cgcctgagcg atgtcgtgct ggccccccac cagttcctcc gcacgtacgc
     3361 ggtaccggaa gacgcgcaac tgtactatac gttcgacgac gcggcaaccg tgcccagcac
     3421 cgcgaccacg cttgtctgcg aagcccattc gcctgccatg gacgggctta ccgcggatac
     3481 ccgcccgacc ttcagccctg acattccgcc ggcgagcacc acccggatca cgcagggata
     3541 caagggaacc gtggtcaatg cggacaacag ctcttcgctg tttttcgtca acacggggct
     3601 gcccgggaac acagactccg cctacggtgg ccaagtcgcc attcccggct ttctgcaccc
     3661 ctccaatttc acggtcgagg tgttcttcaa gatgaaccgg catgtgaact atcccctgct
     3721 tgtcggcaaa gtccgcacag ggtcaagccc tacttggtcg ctctccctgt ccggcgcgga
     3781 tgatttggtc cgctgccggt tcgacacata cccggtagac tggaatctgg gtgagcacac
     3841 ctatgccgaa aattcccaga acggcttcaa ccagtgtttc tctacgggtg tccccggtaa
     3901 cgatggcaag tggcaccatg cggcgttctc ctacgagacc gccaccaaga acgtgaagat
     3961 ctacgtggac tatgtgctga aagcgaacgg caccaccatc aacccgatcg tttacacgga
     4021 cggcgacatc ctgatcggca tgggcgcggg cgaacgggcc tttgacggct ggattgacga
     4081 ggtgcgcatc acgccgaggc tcctgactcc cgacgaattc ctctacacgc agccgcggct
     4141 cggcacgctg atcacccttg attagcgggg gttggggagt gcggcggcat cccgcccttc
     4201 ggatcctcca tgcggcctcc ggatggtttc ccaagacctc gcttgagaca ggagcatcaa
     4261 tctagtatta tggtcgcgtc ttaatgagga ggcgctcgct gtcggcgcgc tgcgtcgcgg
     4321 caagagcctg aacacctttg tgtgcaacgc ttgcatgaag agcgggcacg gtatggagac
     4381 aagcatgcgt gagggaacaa cccggcgggc gtttctggtt caggccggag tggcgggaac
     4441 cgcgctggcc ggggcggccg cgttgacggc gaaggcggga aatcagacaa cggaggcaag
     4501 gaacatggcg gagagcaaga agattctggt ggcggcggac ccgtatgcgg tgtcactgaa
     4561 agacgccgtc gtggcgcatt tgaagggtca gggatacgaa gtcaccgatt tcagcacacg
     4621 cgacggcaag gcgtgcccct attttgaaag tgcggtcgcg gtgtgccggg ccatgcaggc
     4681 cggtcaggcc ccgcgggcga ttctattctg cggcacgggc atgggcatgt cgattgtggc
     4741 caaccgtttc aaaggctgca ccgccgcagt ggtggagtcg gtctacgccg cgaagatgtg
     4801 ccgtgcgatc aacaacgcca atgtgctgtg cctcggccag atgatctggg gtcccgacat
     4861 ggccaaggtc gcggtggatg cgtttctgtc cacccggttc accgaggggc tggaaggcat
     4921 tgcggacttc ctgcgcgatg cgtgcgggaa ggtcgaggcg atccggccgc agtaagccgc
     4981 ggtaccgaag cccaccaccc gcggagcggg aacaaacgcg ggggatgcgg atccacagat
     5041 tacgcagatt gcacagatgg ggccaaggtt ggcgtctcca tcgcggggct gacgtattga
     5101 gatgcgggcg gagcccgcaa tctcaaaggc tgtgaggctt gtgaggctgt gaggctgtga
     5161 gactgtgagg cagtggttaa acagtcgatt ccgatagcga tagcgatacc gataccgatt
     5221 tggatgaaac cctgaaacca gaaaccagaa accctgaaac cttgaaaccc aagagagtgt
     5281 ttcgtggtta aataatcgat cccgataccg ataccgatac cgatttggat gaaaccttga
     5341 aaccctgaaa ccttgaaacc agaaaccc
//
LOCUS       DAOSLE010000168         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13688_length_5363_cov_8.720610, whole genome shotgun sequence.
ACCESSION   DAOSLE010000168 DAOSLE010000000
VERSION     DAOSLE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13688_length_5363_cov_8.720610"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..809
                     /locus_tag="PK576_09510"
     CDS             264..809
                     /locus_tag="PK576_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOE61865.1"
                     /translation="MRVLVPLADGLEELEAVTILDVLRRGGIETVGAALGESLQVQGS
                     RGVKLLADAVWDNLDLESFDALVLPGGGKGTEALAADERVLDAVRAFDAAGRFVAAIC
                     AAPTVLAVAGVLEGRRATCYPSCAPQLGAAFDDAPVIADGNIITSQGPGTAMLFALVL
                     VRHFADEKTARRVADGLLTHF"
     gene            complement(816..2552)
                     /gene="argS"
                     /locus_tag="PK576_09515"
     CDS             complement(816..2552)
                     /gene="argS"
                     /locus_tag="PK576_09515"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOE61866.1"
                     /translation="MMNETVNRCEALLAQWLREAFRSAFGDAQQDWSTLRVQPATDTR
                     FGDFQCNDAMPLAKVLRQPPRQIAEAALRALPLPPMLTSATVAGAGFINLTVNPVWLA
                     GQLAALAATPHFGIPDIGRGQTVVIDYSSPNVAKPMHIGHIRSTVIGNALDRIYRALG
                     YRVIADNHLGDWGTQFGLLIQGYRTFLSDAERETLTVETLEQVYVQSYNRSKEDPAWL
                     DACKAELVKLQQGDPENRAIWERFIAISRQEFDRVYERLDVRFDLTRGESYYNDILPE
                     TIRLLQEKGLAQESEGALVVNLEAEGMPVCIVRKSDGGFNYAATDIATVRSRMAEFRP
                     DRIVYVTDERQQLHFRQFFAVCAKLGCTARLEHVWFGLMRLPEGTFSTRQGNVIKLEV
                     LLDEAERRALEIIRAAHTGMPEAQQRELAAAIGIGAIKYADLSHDPQTLIVFTWEKAL
                     ALEGNSGPYLQYAHARVCSLLDKYAEQVPGSDPNSNTLLLDAPVEKELALRLLQFPAV
                     VTRAAETFKPSVLADYLFGLSQTYSSFYQRSPVLKAEPAVRDSRIRLCAMTAQVLAEG
                     LGLLGIRAPRRI"
     gene            complement(2609..3766)
                     /gene="lpxB"
                     /locus_tag="PK576_09520"
     CDS             complement(2609..3766)
                     /gene="lpxB"
                     /locus_tag="PK576_09520"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOE61867.1"
                     /translation="MSDTPPLRILIGVGEVSGDIHAAALMRALRARLGRPIVFRGFGG
                     DAMRAEGAELLYHTDRIALLGITPVLRNLPFLLGMLRRMKREILTWRPDLVLTVDYPG
                     MNLRLARFAHDRGFRTAHYICPQVWAWRRGRIPKIARSLDLLLCLFPFEPELFSPTAL
                     RAVFTGHPLVDRAAETRAAPPAVLPWGEGHRIALLPGSRSSEITRILPRMLRAAVQLE
                     ARLPGACSFVIPAPTARMRRLGEAVAATMPELPRNIRFTDGQARDTLLQAHAAAVASG
                     TATLEACLMRCPTVLVYAASRITCLLARLFVKGVRHLGLANIVAGREVMPELLQDAFT
                     PERLADALLRYLTDDAARAQVLAGYDEANAKLGTGQASERAAEEIARFFWA"
     gene            complement(3782..4636)
                     /gene="lpxI"
                     /locus_tag="PK576_09525"
     CDS             complement(3782..4636)
                     /gene="lpxI"
                     /locus_tag="PK576_09525"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOE61868.1"
                     /translation="MTTARQVTDNLFIVAGNGTYPACVATGARRAGIRHITMLALRGA
                     TSRATAALADEIAWFGVGEVRRIFDWIARQGFPHAILAGGIPPIALFRTRFDDLARAW
                     LNELPVKNAHTLFGRITTEIERLGIRVLPASCYMDDFLPGAGVFTRRGPTEREARDID
                     FGHRVACGIGGHDIGQTLVVKDGMILAVEAFEGTDAAICRGGKLGGPGAVVVKVAKTG
                     HDMRFDIPVIGKQTIRVLRRARISALAFQAGRLVMLERDKVLAAADRLNLTVVGLDSG
                     LPTAPMRP"
     gene            complement(4587..5321)
                     /locus_tag="PK576_09530"
     CDS             complement(4587..5321)
                     /locus_tag="PK576_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61869.1"
                     /translation="MRRAILTALRFTVYTLVITGAILAIHAAVRYHGKEFFYENNLLE
                     WIQFTVLLTVCLLFAWASCNACGMQHFLILMSMLAAFAATRELDSLLSRFMPVLSWKI
                     GGLFLLYGMWQLIARRKTFVPQILSFVQTPAFVVLWAGFIIAVPFAQLVGHRPFFRMF
                     IEEAQVRDMKRLFEESSEFIGYLILLFGTIETLILFEVFKIGSKSRSRSGSSDADPLP
                     HPSDIRYTAGHDNGTAGDGQPFYCGG"
BASE COUNT         1015 a         1649 c         1758 g          941 t
ORIGIN      
        1 gtccccgccc gctggtaggg acggttcccc gaaccgtccg cggacgcctt agtcccgcat
       61 acgcaccggt ctccaaaact cagtcgatcg cgcttgcgac gaagtggagc gacgaatgct
      121 ccttttagat ttaagagtta gcccgccgcg cgccgagcta cgggaaatcc tgtcaattta
      181 taggcaaggg tcttttgcat tgccgacctt ccgttcacat gccataatat gcggcagagt
      241 agttaaaatc ggaaggagtc cttatgcgtg ttctagtccc tttggctgac gggttggagg
      301 agttggaggc ggtcacgatc ctcgacgtgc tgcgccgcgg cgggattgag accgttggcg
      361 cggccttagg agagtcgctg caggttcagg gctcgcgcgg cgtaaagctt ctggccgatg
      421 cggtctggga caatctggac cttgaatcgt ttgatgcgct cgtcctgccg ggcggcggca
      481 aaggtaccga ggcgctcgcg gcggacgaac gggtgctcga cgcggtgcgg gcgttcgacg
      541 cggcgggccg attcgtggcc gcgatctgcg cggcccccac cgtactcgcc gtggccgggg
      601 tgctcgaagg acggcgtgcc acgtgctatc cctcgtgcgc gccgcagctc ggcgcggcgt
      661 tcgacgatgc gccggtgatt gcggacggca acatcatcac cagccaaggt ccgggcactg
      721 ccatgctctt cgctctggtg ctggtgcgcc attttgcaga cgaaaagacg gcccggcgcg
      781 tggccgacgg tctgctgacg catttctgag acggttcaga tccgacgcgg cgcgcggatg
      841 ccgagcaggc cgaggccctc ggcgagaacc tgcgctgtca tggcgcagag ccggatgcgg
      901 ctgtcgcgca ccgctggttc cgccttcagc acgggtgacc gctgatagaa actgctgtag
      961 gtctgggaga ggccgaacag ataatcggcc agcacgctgg gcttgaacgt ctctgccgcg
     1021 cgcgtcacaa cggcggggaa ttgcagcagg cgcagggcca gctccttttc gaccggtgcg
     1081 tccagcagca gcgtgttgct gttcgggtcg gagcccggaa cctgttcggc gtatttgtcc
     1141 agcagactgc agacacgcgc gtgggcgtat tgcaggtagg ggccggagtt gccctccagc
     1201 gccagcgcct tctcccaagt gaaaacgatc agcgtctgcg ggtcgtggct caggtcggcg
     1261 tatttgatcg cgccgatgcc gatggcggcg gccagctcgc gctgttgcgc ctcgggcatg
     1321 ccggtatgcg cggcgcgaat gatctccaac gcgcggcgct cggcttcatc cagcaacacc
     1381 tccagcttga tcacgttgcc ctggcgcgtg gagaaggtgc cctcgggcag gcgcatcagc
     1441 ccgaaccaca catgctccag acgggcggtg cagcccagct tggcgcacac tgcgaaaaac
     1501 tgccggaagt gaagctgctg ccgctcgtcg gtcacgtaca cgatccggtc gggccggaac
     1561 tccgccatgc ggctgcgcac agtggcgata tcggtcgcgg cgtagttgaa tccgccgtcg
     1621 cttttgcgca cgatgcagac cggcatgccc tcggcctcga ggttcacgac cagcgcgccc
     1681 tcgctttcct gcgccagccc tttttcttgc agcagacgga tggtctcggg caggatgtcg
     1741 ttgtagtaac tctcgccgcg cgtgaggtcg aaacggacgt cgagccgttc gtagacgcga
     1801 tcgaactcct gacggctgat ggcgatgaaa cgttcccaga tcgcacggtt ctccgggtcg
     1861 ccttgctgga gtttcaccag ttcagcctta caggcgtcga gccaggcggg gtcttctttc
     1921 gagcggttgt agctctgcac gtagacctgt tcgagcgttt caacggtcag ggtttcgcgt
     1981 tcggcatccg acaggaaagt gcggtagccc tgaatcagca atccgaactg cgtaccccag
     2041 tcccccagat ggttatcggc gatcacgcgg tagcccaacg cgcggtagat gcggtcgagc
     2101 gcgttgccga tgactgtgga acggatgtgg ccgatgtgca tgggcttggc cacgtttggg
     2161 ctggaatagt caatcaccac cgtctggcct cgtccgatgt ccggtatccc gaagtgcggc
     2221 gtggctgcga gcgccgcaag ttgcccggcg agccagaccg ggttgacggt caggttgatg
     2281 aagcccgcac ccgccacggt cgcggacgtg agcatgggag gcagcggcag ggcccggagc
     2341 gcggcctcgg cgatctgacg cgggggctga cgcagcacct tggccagcgg catggcatcg
     2401 ttacactgga aatcgccgaa ccgcgtgtcc gtcgcgggct gcacgcgaag cgtggaccag
     2461 tcctgctgtg cgtccccgaa cgcgctccgg aacgcctccc gcagccactg cgccaacaac
     2521 gcttcgcacc tgttcaccgt ctcgttcatc atccaccctt acccttacgg ttgaaaaacc
     2581 cgaaggatac ccgcgtccgc gtggcgcgtc acgcccaaaa aaaccgggcg atctcttcag
     2641 cagcgcgttc cgaggcctgt cccgtcccga gtttggcatt ggcctcgtcg tatccggcga
     2701 gaacttgggc gcgcgcggcg tcatccgtaa gatagcggag aagcgcgtcg gccaagcgtt
     2761 ctggcgtgaa ggcatcctgc agcagttcag gcatcacttc ccgtccggcg acgatgttgg
     2821 cgaggccgag atggcgcacg cctttcacga atagccgtgc gagcaggcag gtgatgcggc
     2881 tggcggcgta gaccagaacg gtggggcaac gcatgaggca ggcctcgagc gtggcggtac
     2941 ccgaggccac ggctgcggcg tgcgcttgca gcagcgtgtc acgcgcctgc ccgtccgtaa
     3001 agcggatatt gcgcggcaac tccggcatgg ttgcggcgac ggcctcgccg aggcggcgca
     3061 tgcgggccgt gggtgcgggg atgacaaagg aacacgctcc aggcagccgc gcttcgagtt
     3121 ggactgcggc gcgcagcatg cgtggcagga tccgcgtgat ttcgcttgat cggctgcccg
     3181 gcagcagcgc gatacggtgg ccctcccccc acggcaggac tgcaggcggg gcggcgcgcg
     3241 tctctgcggc gcggtccacc aacgggtggc ccgtgaagac ggcgcggagt gcggtcggcg
     3301 agaacagctc cggctcgaaa gggaagaggc agagcagcag gtcgagcgaa cgcgcgatct
     3361 tcggaatacg gcctcggcgc caagcccaga cttgcgggca gatgtaatgg gcggtccgga
     3421 atccgcggtc atgggcgaaa cgggcgagtc gcaggttcat gccgggatag tcgaccgtga
     3481 gcacgagatc ggggcgccac gtgagaatct cgcgtttcat gcggcgcagc atgccgagca
     3541 gaaagggcag gttccggagc accggcgtga tgccaaggag cgcgatgcgg tccgtgtggt
     3601 agagcaactc ggcaccctcg gcccgcatgg cgtcaccgcc aaagccgcgg aaaacgatgg
     3661 gacgacccag acgcgcgcgc agggcgcgca tcagggccgc agcgtgaata tcgccgctga
     3721 cctcgccgac gccgatcaga atccgtaagg ggggagtatc actcatgcgg gacagccttt
     3781 ctcagggacg catcggggct gtgggcagac cgctgtcgag gccgaccacg gtcaggttca
     3841 ggcggtccgc ggcagccagg accttgtcgc gttcgagcat caccaagcga ccggcctgaa
     3901 aagccagagc cgagatccgc gcccggcgca gcacgcggat ggtttgctta ccgatcaccg
     3961 ggatgtcgaa acgcatgtca tgccctgttt tcgcgacctt gaccaccacg gcacccggcc
     4021 cgcccagttt gccgccgcgg caaatcgccg cgtccgtgcc ctcgaacgcc tcgacagcga
     4081 ggatcatgcc atccttgacc acgagggtct ggccgatatc gtgtccccca atcccgcagg
     4141 ccacgcggtg gccgaaatcg atatcgcggg cctcgcgttc ggtcgggccg cggcgggtga
     4201 acacgcccgc gccgggcaga aagtcgtcca tgtagcagga ggccgggagc acgcggatgc
     4261 cgaggcgctc gatctcagtg gtgatgcggc cgaaaagggt gtgggcattt ttgaccggca
     4321 actcattcag ccaggcgcgg gcgagatcgt cgaaacgcgt acggaagagg gcgatgggag
     4381 ggatcccgcc ggccaggatc gcgtgcggga acccctgccg cgctatccag tcgaaaatgc
     4441 gccgaacctc gccgacgccg aaccaggcaa tctcatcggc cagggccgcg gtggcacgcg
     4501 aggtcgcgcc gcgcagcgcc aacatcgtga tatgccgtat tccggcccga cgcgcacctg
     4561 tcgcgacgca ggcgggatag gtgccattac ccgccacaat aaaaaggttg tccgtcacct
     4621 gccgtgccgt tgtcatggcc agcagtatac cggatatcgg aaggatgcgg caagggatcc
     4681 gcgtcagacg atcccgatcg tgatcgtgat ttggatccga ttttgaaaac ctcaaacagg
     4741 atcagggtct cgatcgtgcc gaaaagcaga atcaggtagc cgataaattc gctgctctcc
     4801 tcgaaaagcc gtttcatatc gcgcacttgc gcctcttcaa tgaacatacg gaaaaacgga
     4861 cgatgtccca ccaactgcgc aaagggcacc gcgatgataa accctgccca gagaaccacg
     4921 aaggccggcg tctgaacgaa agacaagatt tgcggcacaa aggtttttct ccgtgcaatc
     4981 agttgccaca tgccgtagag cagaaaaagc ccgccgatct tccagcttaa aacaggcatg
     5041 aaccggctca gcagggagtc cagttcgcgt gtcgcggcaa atgccgccaa catcgacatc
     5101 agaatcaaaa agtgctgcat gccacaggca ttgcatgatg cccaggcgaa caacagacag
     5161 acggtcagca aaaccgtgaa ctgtatccat tccagcaagt tgttctcgta aaaaaactct
     5221 ttgccgtgat agcggacagc cgcatgtatc gccaagatcg ctccagtaat caccagtgtg
     5281 taaaccgtaa accttaaagc cgttaaaata gcgcgtctca tgtgatctgt ttgcacctgt
     5341 ttggtacgtt acttttttag ggg
//
LOCUS       DAOSLE010000169         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13852_length_5315_cov_9.986692, whole genome shotgun sequence.
ACCESSION   DAOSLE010000169 DAOSLE010000000
VERSION     DAOSLE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_5315_cov_9.986692"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3149)
                     /locus_tag="PK576_09535"
     CDS             complement(<1..3149)
                     /locus_tag="PK576_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOE61870.1"
                     /translation="MAFVLSVCLASRLLGGDNAGADLAGQWQQAQAALEQRDFKAATV
                     LLVSIEQAAMQEREWGHAARAIAQRVELEVATNEMSSGSSALKLKDAASRAPLPMRAV
                     LEVLAARRFRSYAEHLFGGQECDVPVVEGIPQQPEGLLNEIEQCYRRAMAFEEVLKRI
                     PVEMYGALFDEGTVADALRPTLFDLIAYDLLEFYAWCRERKFDDTESVTLDPDGPALQ
                     DVAGFSLVIPDEAARRSRVLRALRLWQTLLAFHVDDKDPAAYADADLNRLQFCYPLVA
                     GEKREERYCAALDRFAHKWRTHELSSRAFALRAQLAFDAGDAALAHVVAQKGASAYPA
                     SIGAAQCRNLIARIEEPSLALASERVWCAPWPQFEIAYKNLTQLHFRAVRVPFDEAFV
                     PRRTRRSAEDATPLQWLARKPLKQWGVALPRVGDYRVHSHRTSVPQDFPNGLYALFVS
                     PDASFEAGGAPIMWETFRVSRLALVVDQKPDRVHGYVLTAREGEPVPAARVTAWRKQV
                     EGGFKRERMMMTAEDGGFAVLGGRAGEIMILAERQDDAAQTWMPLWKGNARMRFEAPV
                     TCAAFVTDRDVYRPGQTLRYRGFFWKDDPAAHSVYAAAGLRVKIAGVDASDRRLAGRE
                     VACTPFGSCSGEMVIPDDTPPGLFTLAAEGIGSTSVQVEAADPSALDVTLALPPPGAR
                     LGDVLTVSGRAVRDGAAVPGARVSWRVAWHREGADPCMAAPLAAGSTVSDTQGVYTVT
                     FFAKAPPQREEADPAACVFTVTVEATAADGGSGRADLPVVLGETAWLAQIAVDAWQTV
                     SQPVRFEVGVQALDGTAIITPGKLRIVQLKPSEWVKRSPLPLLDDSASGVCRVDDGED
                     PVEAWPEMRTLGEERVQTGTNGAASGLVPLPVGVFRLTYETRDPAGRRVTARRNVRVL
                     DPDARCLPFTVPVHVAAESWRVPAGDRFRAVWGSGYARASALMLAESDGFELLRMRTD
                     PEVTQQWFELPVDTAARGGFWFRTLCVRENRAYTYERRVEVVRRDQNLSLAVEGLPPA
                     GCPGERITCTVKVTGPDGAG"
     gene            3653..5098
                     /gene="rpoN"
                     /locus_tag="PK576_09540"
     CDS             3653..5098
                     /gene="rpoN"
                     /locus_tag="PK576_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE61871.1"
                     /translation="MTGPYLSLSQQQRQVMTLAPQLRQSLEMLQMPVMELRTAILKEM
                     AVNPTIDDVTDPTEALLSEVQPENPQEHPTDKELDFDPDVDAILRQDDEWRDYFLQGM
                     ENAPSSEDAEEKRQYLFDSIRQKDSLQDHLMKQLVLTEMSPEDRDLAMMLIGNINDDG
                     YFTGSLPDIIMVSGRSEREVVGVLKVIQSFDPPGVGARTLRECLLQQLTLVEDSPWED
                     EARLLIDKHLEKLGERDESFLCKALALNRDELAHVIALVRSLNPRPGRAFESEVTEYV
                     EPEVFVTWEKGRFVARVDNGQLPHIRISTHYRKLLEDASVSAETKSYIRERIRAGAFL
                     IRSIHQRQETIRKIAQEIVDAQTEFLHKGIEALRPMTMAEVAKKVGVHETTVSRTVAN
                     KYMRTPIGLFEMKFFFTPGLKGKDGVAVSNKAVQDQIMRIVQAEDPTAPLSDQAIEEK
                     LAEQGITVARRTIAKYRGILKIPPSHKRRRT"
BASE COUNT         1122 a         1815 c         1513 g          865 t
ORIGIN      
        1 cccgcgccgt ccggccccgt caccttgacc gtgcaggtga tccgctcgcc cggacaccct
       61 gcaggcggca agccctccac cgccagagag aggttctgat cgcgacgcac cacctctacc
      121 cggcgctcat acgtgtaggc gcggttctcg cgtacacaca acgtgcgaaa ccaaaaccca
      181 ccgcgcgccg cagtgtcgac aggcagttcg aaccattgtt gcgtgacttc gggatcggtg
      241 cgcatccgca gcagttcgaa tccatccgac tccgccagca tcaacgcgga ggcgcgcgcg
      301 taaccgctgc cccaaaccgc gcggaagcga tcgcccgcag gcacacgcca cgattcggcc
      361 gcgacatgta cgggcaccgt aaagggcagg catcgcgcat ccggatcgag cacccgcaca
      421 ttccgccgcg cggtcacgcg ccgtccggcg ggatcgcgcg tttcgtaagt cagccggaac
      481 acgccgacag gcaatggtac caagccggag gcagccccgt tggtgccggt ctgtacgcgc
      541 tcttcgccca acgtccgcat ttccggccaa gcttcaacgg ggtcctcgcc gtcgtcgaca
      601 cggcagaccc ccgaggccga atcgtcgagc agcggcaaag gggaccgctt aacccactct
      661 gacggcttga gttgaacaat ccgcaatttg ccgggcgtaa tgatggccgt gccatccaag
      721 gcctgcacgc cgacttcgaa tctgacgggt tgcgacacgg tctgccaagc gtcgaccgca
      781 atctgcgcga gccacgctgt ctcgcccagc accaccggca ggtccgcgcg tccgctcccc
      841 ccgtcggcgg ctgtcgcctc aaccgtcacg gtgaacacgc atgccgcagg atcggcctct
      901 tctcgctgcg gcggtgcctt ggcgaagaag gtcacggtgt acacgccttg cgtatcgctc
      961 acagttgaac cggcggccaa cggcgccgcc atacacggat cggccccctc tcgatgccac
     1021 gcaacccgcc acgacacgcg ggcacccggc accgccgcac cgtcccggac ggctctcccg
     1081 ctgacggtca gcacatctcc gagccgtgcg ccgggcgggg gcagcgccaa cgtaacatcc
     1141 agcgccgaag gatcggcggc ctcaacctga accgaggtcg atccgatccc ctcggcggca
     1201 agcgtaaaga ggccgggcgg cgtgtcatca ggaatgacca tctctcccga gcaggatccg
     1261 aatggcgtac acgcaacctc ccgcccggcc aaacgtcggt ccgatgcatc cacccctgca
     1321 atcttgacgc gcaaaccggc cgccgcatac acgctgtgcg ccgccgggtc atccttccag
     1381 aagaaccccc ggtagcgcag agtctggccg gggcggtata cgtcacggtc ggtcacgaat
     1441 gcagcgcacg tcacgggggc ttcaaaacgc atgcgcgcat tccccttcca aagcggcatc
     1501 cacgtttgcg cggcatcgtc ctgccgttcg gcaagaatca tgatctcccc tgcgcgaccg
     1561 ccgagcacag cgaatccgcc gtcctccgcc gtcatcatca tccgctcgcg tttgaagcct
     1621 ccttccacct gtttgcgcca cgccgtaaca cgcgccgccg gaaccggttc gccctcacgc
     1681 gccgtcaaca catacccatg gacgcgatca ggtttctgat cgaccaccaa cgccagacgc
     1741 gagaccctga acgtctccca catgatcggc gcacccccgg cctcgaaaga tgcgtccggc
     1801 gaaacgaaca gcgcgtagag tccgttcggg aagtcttgcg gcaccgacgt gcgatggctg
     1861 tggacccgat aatcccccac ccgcggcaac gccactcccc actgcttcag cggcttgcgc
     1921 gccagccact gcaatggcgt cgcgtcctcc gccgaccgcc gggtgcggcg cggcacaaac
     1981 gcctcgtcga acggcacacg caccgcacgg aaatgaagct gcgtcaggtt cttgtaggcg
     2041 atctcgaact gcggccacgg cgcacaccag acacgttctg aagcgagcgc gagagaaggc
     2101 tcctcgattc gagcgatcag attacggcat tgcgcagcac cgatgctcgc cggatatgcg
     2161 gacgcgccct tctgcgcaac gacatgcgcc agagcggcat cccccgcatc gaacgcgagt
     2221 tgcgcacgga gggcgaacgc acgcgacgac agctcgtgcg tccgccactt gtgcgcgaac
     2281 cggtccagcg ccgcgcagta tcgctcctcc ctcttttcac ccgccaccag tggatagcag
     2341 aactgcaggc ggttcaaatc agcatcagca tacgcggcgg gatccttgtc gtccacgtgg
     2401 aaggcgagca acgtttgcca caggcgtaac gcccgcaaca cacgggaacg gcgcgctgcc
     2461 tcatcgggga tcacaaggct gaaaccggca acatcttgca gggcgggacc gtcagggtcc
     2521 agtgtcaccg actccgtgtc atcaaacttc cgctcccggc accacgcata gaactccagc
     2581 aggtcatacg caatgaggtc gaacagcgtc gggcgtaacg catccgccac ggtaccctca
     2641 tcaaacaagg ccccgtacat ctcgacggga atccgcttaa gcacctcctc aaaagccatc
     2701 gcgcgccggt agcactgctc gatctcgttc agcaaacctt ccggctgctg cggtatgcct
     2761 tcgactaccg gaacgtcaca ttcttggccg ccgaaaagat gttccgcata cgaccggaag
     2821 cggcgcgcgg ccaggacctc cagcacggcc cgcatgggaa gcggcgcacg ggacgccgca
     2881 tccttcagtt tcagcgcgga tgagccggaa gacatttcat ttgtcgccac ctccagttcg
     2941 acacgttgcg caatggcacg cgcggcgtgt ccccactccc gctcctgcat tgccgcctgc
     3001 tcgatcgaca ccagtagcac cgtcgccgcc ttgaagtccc gctgttccaa agccgcctgc
     3061 gcctgctgcc attggcctgc aaggtcggct ccggcattat cgcccccgag caagcgcgag
     3121 gctaaacaca ccgacaggac aaacgccacc gcggtgcaca atcggttaat gacaggggtc
     3181 atgcgagccg ggagcgtgtt ttgtatgtgc gcagtcggga gcgcgggcgt ccccgcccgc
     3241 tctgtgaaag accgattgag attccgatgc gcggtttggc tcccaaggga gcaaaaaaaa
     3301 cgtagcatgg gcgtcccgcc catgaaacac gggcaagatg cccgtgccac acccttttgc
     3361 gaggcacgtt gttgcggacg aggacgcccg cgcccccgac tctctgttcc ccttttcacc
     3421 agaaacgcat gaccttcgca cacccctgtc actaaccgat tacccgcggt acgcgcaaaa
     3481 cgcaaaccat ctgattgcat ggcttggaga ttaccaagat gccccatttg cggcaaggac
     3541 actgagcgaa ggcttgctcc ctcgactccg ttccactatg atactggcat tttacaaaac
     3601 gtggtacgga tgatgcttgc agagaatggg cgtcttcctg aacggcactc gcatgacagg
     3661 tccatatctt agtctcagcc agcagcagcg tcaggtgatg acgcttgcgc cgcaactgcg
     3721 ccaatcactg gagatgctcc agatgccagt gatggagttg cgtaccgcga ttctcaaaga
     3781 gatggccgtc aacccgacga tcgacgacgt cacagatccg accgaagcct tgctgtctga
     3841 ggtccagccc gaaaacccgc aggagcaccc caccgataag gagctcgact tcgaccccga
     3901 cgtggacgca atcctgcggc aggacgacga atggcgcgac tacttcctgc agggcatgga
     3961 aaacgcgccg tcgtccgaag acgccgaaga gaagcggcag tatcttttcg actccatacg
     4021 ccagaaagat tcgctgcaag accacctgat gaagcagctc gttctgaccg agatgtcgcc
     4081 ggaagaccgg gatctggcca tgatgctgat cggcaacatc aacgatgacg gctacttcac
     4141 tggcagcctg ccggacatca tcatggtctc cggccgtagc gaacgcgagg ttgtcggggt
     4201 ccttaaagtg atccagtcgt tcgatccgcc cggtgtcggc gcacggacgc tacgcgagtg
     4261 tctcctgcaa caactcactc ttgtagaaga ttcaccgtgg gaggacgagg cgcgtctgct
     4321 gatcgacaag catctggaga aactcggcga gcgcgatgaa agttttctct gcaaggcgct
     4381 cgcgctgaac cgcgatgagt tggcgcatgt gatcgcgctg gtgcgcagcc tcaatccccg
     4441 cccgggacga gcctttgaga gcgaggtgac agaatacgtc gagccggaag tttttgtaac
     4501 ttgggaaaag gggcgtttcg tagccagagt ggacaacggc caactgccgc acatccgcat
     4561 cagcacgcat taccgcaagc tactggaaga cgcgagcgtt tcagccgaga ccaagtccta
     4621 cattcgcgaa cgcatccggg ccggcgcgtt tctgatccgc agcatccacc agcgccaaga
     4681 gaccatccgc aagatcgcac aggagattgt cgatgcccag accgaattcc tgcacaaggg
     4741 catcgaggct ctccgtccca tgaccatggc cgaagtcgct aagaaagtgg gcgtgcatga
     4801 aaccacggtc agccgtaccg tggccaacaa atacatgcgc acaccgatcg gcctgtttga
     4861 gatgaaattc ttcttcacgc ccggcctgaa aggcaaggac ggcgtggccg tctccaacaa
     4921 ggcggtgcag gatcagatca tgcgtatcgt gcaggccgaa gacccgacag caccgctttc
     4981 cgatcaggcg atcgaggaga agctggccga gcagggtatc accgtcgcac gccgcaccat
     5041 tgccaaatac cgcggcatcc tcaagatccc gccatcgcac aaacgccgcc gcacttgatg
     5101 atcacttgcg agtgccgaaa agagacggag cggggacgag tagacgctcg tccagtgctg
     5161 gagaaaacgc atgccggcga atgtccccac gaactacacg ggtcgcgaca ccagaaacgc
     5221 ttgacccgtt ttgacgtatc gcctacgcta tagatcacca ctcgctggaa acggaggcat
     5281 gcggcgcggg accgccgcag aaatccgaca ccacc
//
LOCUS       DAOSLE010000170         5303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_13898_length_5303_cov_7.639101, whole genome shotgun sequence.
ACCESSION   DAOSLE010000170 DAOSLE010000000
VERSION     DAOSLE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13898_length_5303_cov_7.639101"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..3553
                     /locus_tag="PK576_09545"
     CDS             173..3553
                     /locus_tag="PK576_09545"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61872.1"
                     /translation="MTFKHMLFLVPLASLAGTIQIPGSWSMPTEERDAVTLNALPPAV
                     PRKASGMRTTLAQASVRTAEDVSVAELKGFPGVRIAEGEIVLAYAPVTKTSAFAAMAK
                     SDATQKWYGSDYWIGSEGWARVGRDWMHPGHAVDVARTFIAPVNGRVSVSGTVRKLHL
                     DGDGVKAFVQHNNRTLWTAEVKGRDAVGQQHALTVEVKKGDTLRFLVNRLGSHACDTT
                     GWDPVVAYEGGGVHRASEGFSDKQEANGWRYEFSGGPAAQTSLPTIYGVGRDLKLYRN
                     AIDTVNALPFVIVSDGNKEDGFIGYVIARDSDAAWTLIRDPGGIKVAGAGAAVVVRYD
                     GTWIEGLTLLAALSGVETSPQRVRDGFGGAVLPKLGSVFRAAYAKAVAGVPHAPEPDL
                     FLLAVSEWVQDDQLFGCTDEQRLTACAKEQAERIEQITGKPDASAGTGWGGYLRARLL
                     KRAALLSDPRLAYGELLFCTRRNPAWNHEVAQYFGWRQRPGGSLFVLEKPGVSLMRRD
                     LINGRLPPGNLLEPRLSYDARSVLFSFVATDGALDPRTLPRNETDTNHHYYHLYEIGA
                     DGTGLRQITDDIYDDLMPEWLPDGGIAFVSTRRRTTSRCFWWSYSDRWHSYTMFRMAR
                     DGSDIRQLSWNDVNDWFPAVANNGHLLFARWDYIDRDAVTHQNLWSMRPDGTNPMAVW
                     GNEVPKPHCTFQAKAIPNSGKIVCVASAHHAITGGPIMLIDPAVHNNSEAAVTRLTPG
                     HYPEAERGSNNEWYNAPWPLGEDLFLVAYSRDPLMYEPARHNPDYALGLYVLDACGNR
                     ELLYRDGLIGATCPTPLRPRPVPPVLPSVLNPALAQREQGEVFISDVYEGIPKAKRGT
                     IKKIRVVQVFPKTTRDSMVPRIGVAGEENTRAVLGETPVEADGSARFLVPACKPILFQ
                     VVDADGCAYRVMRSSTSLMPGERVSCVGCHENKMAVSPAALKKVAAMMRPAAELTPTP
                     ESGRPYGFVEQVQPLFDAKCVSCHGDRNPKGGIALTRTLRGAYTASYDSLCYATDSEG
                     KRVRRLSKKSGRPLVTCYPMRNQIQVTPEGGEDSARGSGLFQMLKEGHGKVRLTPEEL
                     RRIAVWIDLNAVFYGVYEPEAQLAMQREGKPVPMPAIQ"
     gene            3660..5126
                     /locus_tag="PK576_09550"
     CDS             3660..5126
                     /locus_tag="PK576_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE61873.1"
                     /translation="MRILHGFFGTGLGLRHIIPMLAVLGGARAADYYVSPEGRDTDPG
                     TESSPWQTIAKASATMTAGDTVHIMKGVYRECVRPAHSGLPGQPITYTAYPGHSAVID
                     GIGVALAKRGGILGLVDIGNKKHIRISGLTVINTMEQNAAGIFAAGCEDLILENNRIA
                     NTGASGILTYRCSKVLIDGNRVQNVCVRGGQESISLMYTDTFDVRSNYVHSTPPSLTG
                     KGGWLYRKEGIDAKDGSSHGRIFGNHVHHTSLGIYVDARGTARDIEISGNIIHDIFSS
                     FEEPGGQGTGNGIVIASETEGTIENIRIFNNLIYNTHFGIRLAGYGDPGHPVMTNLTV
                     IHNTLYRTRRGIELSSRNATGIVIRNNLCSRSTDFDIGIFRNSWGDQPRMVLDDPTLC
                     VANNLLDVPAGAGRPWERDNLYGAANFVAPEAGDFRLRADSAARDRGTAAGMPSVDIT
                     GTPRPVGAAPDIGAYEFTGPGVKTESASPAIPAARPQP"
BASE COUNT         1047 a         1683 c         1673 g          900 t
ORIGIN      
        1 atcttacgga cgtggagatc cgcgccgatg atgatccgga gttccaaggc tccgactggc
       61 tggccatccg gccgcgcttg aaaaccgcac cgatcgtgat ccggccggga cctttccgcg
      121 cgaacgcgca gggaatcgag tgaggaagaa aacgaattcg aggagaaaaa cgatgacctt
      181 caagcacatg ctgtttcttg tgccgcttgc ctcgctggcg ggcacgattc aaatcccggg
      241 ttcctggtcg atgccgacgg aggagcgcga tgccgtcaca ctaaatgcgc tgccgccagc
      301 cgtgccgcgc aaggcgtccg gcatgcggac cacactcgcg caggcctccg tgcggacagc
      361 agaggacgtg tccgtggcgg aactgaaagg gtttccgggc gtgcggatcg cggaaggcga
      421 aattgtgctg gcctacgccc cggtgacgaa aaccagcgct ttcgcggcca tggccaaatc
      481 agacgcgacg caaaaatggt acggctctga ttactggatc ggttccgaag ggtgggcccg
      541 cgtgggaagg gactggatgc atcccggcca tgccgttgat gtggcgcgca cctttatcgc
      601 gcccgtcaac gggcgcgttt cggtctccgg aacggtcagg aaactacacc tcgacggcga
      661 cggcgtgaag gcctttgtgc aacacaacaa caggaccctc tggacagcgg aagtgaaagg
      721 gcgggatgcg gtcgggcagc aacacgcgct cacggtcgaa gtcaaaaaag gggacacgct
      781 ccgcttcctc gtcaatcgtc tggggtccca tgcgtgcgac actacgggct gggatccggt
      841 cgtcgcgtac gagggcgggg gcgtacaccg ggcctccgaa ggcttttcgg acaaacagga
      901 ggcgaacggc tggcgttacg agttttcggg aggaccggcc gcgcaaacct ccctgcccac
      961 tatatacggg gtgggccggg atctgaaact ctaccgcaac gcgattgaca cggtaaacgc
     1021 cctgcctttt gtgatcgtct cggacggcaa caaggaggat ggctttatcg gctatgtgat
     1081 cgcacgggat tcggatgccg cctggaccct gatccgcgat ccggggggga taaaggtggc
     1141 gggtgcgggc gcggcggtgg ttgtgcgtta cgacggcaca tggatcgagg ggcttacgct
     1201 gctggctgcc ttgtccgggg tagaaacgtc tccccaaaga gtccgcgacg gtttcggggg
     1261 agccgtcctg ccgaagctcg gctccgtgtt ccgcgccgcc tatgcgaagg ccgtggccgg
     1321 cgtgccgcat gcgccggagc cggacctgtt cctgctggcg gtctcggagt gggtgcagga
     1381 tgaccaactc ttcggttgca cggatgagca acggctcacc gcgtgcgcaa aagaacaggc
     1441 ggaacggata gaacagatca cagggaaacc cgacgcgtcg gcaggaacag gatggggagg
     1501 atacctgcgg gcgcggctcc tgaagcgcgc ggccctgttg tccgatccca gactcgcgta
     1561 cggcgagttg ctcttctgca cgcgccgcaa tccggcttgg aaccacgagg tggcacagta
     1621 tttcggttgg cggcagcggc cgggcggcag cctgtttgtg ctggagaaac ccggcgtctc
     1681 cctgatgcgg cgagatctga tcaacggaag gctccctccc ggtaaccttc tcgaaccgcg
     1741 cctgtcctac gacgcccgct ccgtcctttt ctcttttgtg gcgacggatg gcgcactcga
     1801 tccgcgtacg ttgccgcgca acgagaccga cacgaaccac cactactatc acctttacga
     1861 gattggtgcg gacggcacag gcctgcgcca gatcacggat gacatctacg acgacctgat
     1921 gcccgagtgg ttgccggacg gcggcatcgc ctttgtctcc acgcgccgcc gcaccacctc
     1981 acgctgtttc tggtggagct acagcgaccg ctggcactcc tacaccatgt tccgcatggc
     2041 gcgcgacggt tcggacatcc ggcaactctc ctggaacgac gtgaacgact ggttcccggc
     2101 ggtcgccaac aacggccatc tgctcttcgc gcgctgggat tacatcgacc gcgatgcggt
     2161 cacccaccag aacctctggt ccatgaggcc cgacggcacc aaccccatgg ccgtatgggg
     2221 caacgaggtg cccaagccgc actgcacctt tcaggccaag gccatcccca acagcggcaa
     2281 gatcgtgtgc gttgcctccg cgcaccacgc gatcaccggc gggccgatca tgctgatcga
     2341 ccccgccgtg cacaacaaca gcgaagccgc ggtgacgcgc ctgaccccgg gccactaccc
     2401 cgaagcggaa cgcggctcca acaacgagtg gtataacgcg ccctggcctc tcggcgaaga
     2461 tctgtttctc gtggcctaca gccgcgaccc gctgatgtac gagcccgcgc gccacaatcc
     2521 tgattacgcc ctcgggctct acgtgctcga cgcctgcggc aatcgcgaac tgctttaccg
     2581 agacggcctg atcggtgcga cctgcccgac gccgcttcgg ccgcgccccg tgccgccggt
     2641 cctgccctct gtcctgaatc ccgcgctcgc gcaacgggaa cagggtgagg ttttcatctc
     2701 ggatgtctat gaaggcatac cgaaggcgaa acgcggcacg atcaaaaaga tccgcgtggt
     2761 gcaggtcttc ccgaaaacca cacgggactc gatggtgccg cgcatcggtg tggcgggcga
     2821 ggagaacacg cgcgcggtac tcggcgaaac gccggttgag gcggacggct cggcccgttt
     2881 ccttgtgccc gcctgcaaac cgatcctctt tcaagtcgtg gatgccgacg gttgcgcgta
     2941 ccgggtcatg cgttccagca cctcgctcat gcccggcgag cgcgtctcct gcgtcggctg
     3001 ccacgaaaat aaaatggccg tctctcccgc ggcactgaaa aaagtggcgg ccatgatgcg
     3061 tcccgcggcc gagctgacac ccacgccgga gtcgggacgc ccgtacggct tcgtcgagca
     3121 ggtgcaaccg ctcttcgacg ccaaatgcgt ctcctgccac ggcgaccgga atcccaaagg
     3181 aggcatcgcg ctcacccgga cgctccgggg cgcatacacg gcgtcctatg attcactctg
     3241 ttacgccacg gattcggagg gcaagcgcgt gcgccgcctc tccaaaaaga gtggccgtcc
     3301 gctggtgacc tgctatccta tgcgtaatca gattcaggtc acacccgaag gcggcgagga
     3361 cagtgcgcgt ggcagcggac tcttccagat gctcaaggag gggcacggca aggtcaggct
     3421 gactccggag gagttgcgcc gcatcgcggt gtggatcgat ctgaacgccg tcttctacgg
     3481 cgtgtacgag cccgaggcgc aacttgcgat gcagcgcgag ggcaagcccg tgcccatgcc
     3541 ggcgatccag tgagcctgcg gcggggccca agcgtgtggc gtccgatgtg tgcggggaac
     3601 aggattaaga caccggtcac gaccgggacc ggaaaggaca gggaaaaaag gaggaaagca
     3661 tgcggattct acacgggttt ttcggaacgg gattggggct tcggcacatc atccccatgc
     3721 tggcggtttt gggcggggcc cgcgcagcgg attactacgt cagtcccgag ggccgggata
     3781 cggatccggg caccgagtcg agtccgtggc agacgatcgc caaggcttcc gcgacaatga
     3841 ccgccggaga cacggttcac atcatgaaag gcgtctatcg ggaatgcgtg aggccagcgc
     3901 actccggatt accgggccaa ccgatcacct acaccgcata tcccggacat tcggcggtca
     3961 tcgacgggat cggcgtcgcg ctggccaagc gcggcggcat cctcggtctg gttgacatcg
     4021 gcaacaagaa gcacatccgg atttccggac tgaccgttat caacacgatg gaacagaatg
     4081 ccgcaggcat cttcgccgcc gggtgcgaag acctcatttt ggagaacaac cggatcgcca
     4141 acaccggcgc atccgggatc ctcacctacc gttgctcgaa ggtcctcatc gacggcaacc
     4201 gggttcagaa tgtctgcgtc cgaggcggcc aggagagcat ttcgttgatg tataccgaca
     4261 cgttcgatgt ccggagcaac tacgtgcaca gcacgccgcc cagtctgacc ggcaaaggcg
     4321 gctggcttta ccggaaggag gggatcgacg cgaaagacgg ctcttcccac ggacggatat
     4381 tcggcaatca tgtgcaccac accagtctcg gcatttatgt cgacgcccgc ggaacggcgc
     4441 gcgacattga gatctccgga aacatcattc acgatatttt ctcctcgttc gaagagcccg
     4501 gcggacaggg cacgggcaac ggcattgtga tcgcctctga aacggagggc accatcgaga
     4561 acatccggat tttcaacaac ctgatctaca atacacattt cggaatccgt ctggcggggt
     4621 atggcgaccc gggccacccg gtcatgacga atctcactgt gatccacaac acgctgtacc
     4681 ggacacgccg gggaatcgaa ttatcgagtc ggaacgcaac gggcatcgtg attcggaaca
     4741 acctctgcag ccgcagcacc gactttgaca tcggcatctt ccggaactcg tggggggacc
     4801 agccgcggat ggttctggat gacccgaccc tctgcgtcgc aaacaacctg ctcgacgtcc
     4861 cggcaggggc gggacggcca tgggaacgcg ataacctcta cggcgcggcc aactttgtcg
     4921 ccccggaagc cggggatttc cgcctgcgcg cggattccgc ggcgcgcgac cggggcaccg
     4981 ccgccgggat gccgtcggtt gacatcaccg ggaccccgcg accagtcggt gccgcgccgg
     5041 acatcggcgc ctatgagttc acggggcccg gagtcaagac ggagtcggcc agccccgcta
     5101 tccctgcggc gcggccacag ccgtgagccg tcccctaggt tttcgcgcgc gcatgagcgg
     5161 ttgccccgaa cttactgccc cgcccgatag cgatcccgat agcgatagcg atcccgattt
     5221 agatgaaacc atgaaaccat gaaacccaga aaccagaaac ccttgaaccc ccgtcgttgt
     5281 gagccactgc gtggtcacaa taa
//
LOCUS       DAOSLE010000171         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_14052_length_5255_cov_6.425962, whole genome shotgun sequence.
ACCESSION   DAOSLE010000171 DAOSLE010000000
VERSION     DAOSLE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14052_length_5255_cov_6.425962"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PK576_09555"
     CDS             <1..357
                     /locus_tag="PK576_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOE61874.1"
                     /translation="LDAPPGTSCPMVATLREADFVVLVTEPTPFGLHDLRLAVAVVRE
                     LDIPCGVVINRAGSGDDNVTAYCRDEKIPILAEIPDDRRVAEAYSRGELMVDALPEYR
                     AVFEALYSEVKGEEKV"
     gene            354..1265
                     /locus_tag="PK576_09560"
     CDS             354..1265
                     /locus_tag="PK576_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE61875.1"
                     /translation="MKKGKAITEWVVISGKGGTGKTSITASFAALAAGRAVLADCDVD
                     AADLHLVLTPEILERHDFVNGREAVIRADDCVGCDVCADLCRFDALRQARHPSGKAIY
                     KIDPIGCEGCGVCVRFCRSKAIDFPDRLCGEWMLSETRCGPMVHACLYAAAENSGKLV
                     ATVRREARRIAEAKDLPLIITDGPPGIGCPVIASLTGASKVLAVTEPTVSGEHDLERV
                     LQLARFFGIPVAVCVNKWDINPDVTERIERKAENLGAACIGRVRYDKAVTTAQIQRKS
                     VVEIESAAGDDIRQIWEEMEGSWRERA"
     gene            1380..1733
                     /locus_tag="PK576_09565"
     CDS             1380..1733
                     /locus_tag="PK576_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE61876.1"
                     /translation="MSKNKIKIAIPMAEDKLCLHFGHCERFALLDVDLTGRKIISRAD
                     AVPPPHEPGVLPRWLAEQKVEMVMAGGMGQRAQGLLAGQNIRVIVGLSPDTPENIIAQ
                     YLDGTLKSGENVCDH"
     gene            1742..2572
                     /locus_tag="PK576_09570"
     CDS             1742..2572
                     /locus_tag="PK576_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE61877.1"
                     /translation="MGQKVSITILADNLAAEGLASEHGFAAWIETDGAKILFDTGNGD
                     AMMQNAETLGIALPHADMIVLSHGHNDHTGNLATILQQAPQARLYLHPEALRQRYSIR
                     ESAKPIGMPASAVNAVSGLAEHRRMWVTATVQISEKVRLTGPVPRRTAYEDTGGPFFK
                     DAAGAHPDDIPDDMSLWIETRAGLVVCLGCCHSGVINTLNHITAVSGERRIRCVVGGT
                     HLVHASAERMKQTSAALKAYNIPRVVPCHCTGEDAAFFLKEELGETVHQGYAGMRIDI
                     "
     gene            2758..3270
                     /gene="tsaA"
                     /locus_tag="PK576_09575"
     CDS             2758..3270
                     /gene="tsaA"
                     /locus_tag="PK576_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOE61878.1"
                     /translation="MSDNQIIFRPIGIIRTEHTNPEATPIQPIFAKDCEGCVMVAPEF
                     VAGLADIEDFSHVYLVYLLHKVSRVTLVTKPFMQDKEHGVFATRSPCRPNPIGLSIVR
                     LVSRDGGTLIVKGADMLDGTPLLDIKPYTRRFDNISTERNGWQDEVDDATAAKLGVRN
                     YCNRHLSSAE"
     gene            3374..4288
                     /locus_tag="PK576_09580"
     CDS             3374..4288
                     /locus_tag="PK576_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOE61879.1"
                     /translation="MNPEKTACDQSQCNACSDASCPSAGRQAQQPPMPDDARLKNRLS
                     RIRRKVVVMSGKGGVGKSTVAVNLAMSAMLSGQKVGLLDIDLHGPSVPVMLGLVGQRL
                     EGGPDGMLPVEIGGLKVLSVGFLLNEQDDPVIWRGPMKMGVIRQFIEDVDWGDLDLLV
                     VDVPPGTGDEPLSICQLIPELDGAVIVTTPQKVAAADVRKSINFCKELGVAVLGVVEN
                     MSGFACPKCGEVTPILSADGGRVTADEMGIPFLGSIPIDPLIAESGDNGKPFVESAAS
                     SPAAGAFREIVRVLTVTPTLSQEKPACV"
     gene            4503..>5255
                     /locus_tag="PK576_09585"
     CDS             4503..>5255
                     /locus_tag="PK576_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61880.1"
                     /translation="MRTMTGLGLALLLVGGGQCADFYVATNGADRADGTREKPLATLA
                     AARDAARRSGGGGHRIRLLPGEYFLEEPLTLDARDNGLTVEAEGAGRVTVYGGRRVTG
                     WHRDGEKFWCADLPEVKSGAWDFRALVVNGRMPDRARMPESGTFTHQNVFDAKWLSSV
                     GGGWERKPTHADLTTLVYHPTNLPPSLEVRNAELRVYHMWDDSLVGVASNDVARHTLH
                     FTSPAGHPAGAFGVKKYVVFNTREGMTRPGQWY"
BASE COUNT         1100 a         1376 c         1748 g         1031 t
ORIGIN      
        1 ctggacgcgc cgcccggcac ctcgtgcccg atggtcgcca cactgcgtga ggccgatttt
       61 gtggtgctgg tcaccgagcc aacacctttc ggtttgcacg acctgcggct ggccgtggcg
      121 gttgtgcggg aactggacat accctgcggt gtcgttatca accgcgctgg cagcggggat
      181 gacaacgtca ccgcctactg ccgggacgag aagattccga tactggccga aatcccggat
      241 gaccgccgcg ttgcggaagc ctattcgcgc ggcgagctga tggtggacgc gctgccggaa
      301 taccgcgcgg tgttcgaagc gttgtacagt gaagtgaagg gggaagagaa agtttgaaaa
      361 aaggaaaggc gatcacggaa tgggttgtca tcagcggcaa gggcggcacg ggcaagacca
      421 gcattaccgc ctctttcgcc gcgttggctg ctggccgcgc ggtgttggcc gactgtgatg
      481 tggatgcggc tgacctgcat ctggtgttga cgccagagat acttgaacgt catgatttcg
      541 tgaacgggcg tgaggctgtg atacgggccg atgactgcgt cggctgcgac gtgtgcgctg
      601 acctttgccg tttcgatgcg ctgcgccaag cgcggcaccc ttccggcaag gcgatctata
      661 agattgatcc gatcggctgt gaggggtgcg gcgtgtgtgt caggttctgc cggtccaaag
      721 cgatagactt ccccgatcgg ctctgcggcg aatggatgct gtcggagacg cgttgcgggc
      781 cgatggtcca cgcctgcctg tacgcggcgg ccgagaactc cggcaagtta gtggcaacgg
      841 tgcggcgcga ggcgcgccgt atcgccgagg ccaaagatct gccgctgatc atcaccgacg
      901 ggccgcccgg tatcggttgc ccggtgatcg cgtcgctcac cggcgcctcc aaagtattgg
      961 cggtcaccga gccgaccgtc tccggcgagc atgatctgga acgtgtgctg caactcgccc
     1021 gattcttcgg gattcctgtc gcggtatgcg tgaacaaatg ggacatcaac cctgatgtga
     1081 ccgaaaggat cgagcgcaaa gctgaaaacc tcggtgccgc ctgtattggc cgcgtgcggt
     1141 atgacaaagc cgtgacaacg gcgcagatcc aacggaagtc tgtggtggag atcgagtcgg
     1201 cagccggaga cgacatccgg cagatttggg aagagatgga gggttcatgg agggagcgcg
     1261 cgtaaacgcg ctacacaaga cggcaggccc ggcagtaccc gcagcggttt cggcggttcg
     1321 agacatcccg ccgtactggt atggggcggg gaatatggat ttacaaggaa ggagcaatga
     1381 tgagtaagaa taagatcaag attgcgatac cgatggcaga agataagctg tgtttgcatt
     1441 tcggccactg cgaacggttc gcgctgttgg acgtggattt gacggggcgc aagatcatca
     1501 gccgcgcgga tgcggtgccg ccgccgcacg agccgggcgt actgccgcgc tggctggcgg
     1561 agcagaaggt ggagatggtt atggccggcg gcatgggaca gcgggcacag gggcttttag
     1621 ccgggcagaa catccgtgtg atcgtggggc tctcgcccga cacccctgag aatatcatcg
     1681 cgcaatatct ggacggcaca ctgaagtccg gcgagaatgt ctgcgaccac tgaaaaaggc
     1741 tatgggccaa aaagtttcca tcaccatctt ggcggacaac ctcgcggctg aagggctggc
     1801 gtctgaacat ggtttcgctg cctggattga aaccgacggc gcaaaaatcc tgttcgacac
     1861 cggcaacggc gatgccatga tgcagaatgc cgagacgttg ggtatcgcct tgccgcacgc
     1921 cgacatgatc gtattaagcc atgggcataa tgatcacacc ggcaatctcg ctacgatctt
     1981 gcagcaagcg ccgcaagcgc ggctctatct gcatccggag gccttgcggc aacggtacag
     2041 catccgtgag agtgccaagc cgataggcat gcccgcaagc gcggtgaatg cggtctctgg
     2101 gcttgcggag cacagacgta tgtgggtcac ggccaccgtg cagatttctg aaaaagtccg
     2161 gctgacagga ccggtgccgc gcagaaccgc atacgaggat acgggagggc cgtttttcaa
     2221 ggatgccgca ggcgctcacc ccgacgacat tcccgacgat atgtcgctct ggatcgaaac
     2281 ccgcgcaggc ttggtggtct gtctgggttg ctgccactcc ggcgtgatca acacgttgaa
     2341 ccatatcacg gctgtttccg gcgagcggcg gatccgttgt gtcgtgggcg gcacacactt
     2401 ggtgcacgct tccgccgaaa gaatgaagca gacaagcgcg gcattgaaag cttacaatat
     2461 cccgcgtgtt gttccttgtc attgcaccgg cgaggatgcg gcattttttc tgaaagagga
     2521 acttggcgaa acagtgcatc agggttacgc gggtatgcga atcgatatct gatgcggcaa
     2581 aaaggatgat acatgagcaa gaagctgaaa gtggttgtgg tcggcgggct ggttgcctgg
     2641 ccctacggaa tcgtccgcgg cgcgtgagga cactcgccct ccaagaaccg ccgcaggtcg
     2701 catgtcgcct gtcgcggtat gggcagaaaa agaactacgc aaggaaagaa ggtgtttatg
     2761 agtgacaacc aaattatttt tagaccgatc ggaataatcc gtaccgagca cacaaatccg
     2821 gaagccacgc caatacaacc gatattcgcg aaggactgcg agggatgtgt catggttgcc
     2881 ccggaattcg tggcgggtct ggctgacata gaggatttct cgcatgtata tctagtgtac
     2941 cttctacaca aagtaagtcg tgtgactctg gtcaccaaac ctttcatgca ggacaaagaa
     3001 cacggcgtat tcgctacgcg ttcgccatgc cgcccgaacc cgatcggttt gagcatagta
     3061 cgacttgtca gccgtgatgg cggcaccctg atcgtaaaag gagcggatat gctcgacggt
     3121 acgccgctac tggacatcaa gccctacacg cggcgttttg acaatatttc tactgagcgt
     3181 aatggctggc aggacgaagt ggacgatgcc actgccgcaa aacttggagt ccgtaactac
     3241 tgcaatcgac acttgtcatc agcagaatga aaccacagga aaaccaaggc agaagagctt
     3301 ttagggacgt ggggttctgc tagtcccctt ttggactttg aagcgacaaa atatgaattt
     3361 tgaaaggagt taaatgaacc cagaaaaaac ggcgtgtgat caaagccaat gcaacgcgtg
     3421 cagcgatgcg tcgtgtccct ctgcaggcag gcaggcgcaa cagccgccca tgcctgacga
     3481 tgccagactg aagaaccgtc tgagccggat ccggcgcaag gtggtcgtga tgtccggcaa
     3541 aggcggcgtg ggaaagagca cggtggcagt gaacttggcg atgtccgcta tgctctcggg
     3601 ccagaaagtc gggttgctgg acatcgacct gcacgggccg agcgtaccgg tcatgctcgg
     3661 tctggtcggc cagcggctgg aaggaggccc ggacggcatg ttgcccgtgg agatcggcgg
     3721 tctcaaggtg ctttcggtcg gttttctgct gaacgagcag gatgatccgg taatctggcg
     3781 cggccccatg aaaatgggcg tgatccgcca gttcatcgag gatgttgatt ggggcgacct
     3841 tgacctgctg gtggtggacg tgccgccggg caccggcgac gagccgctct cgatctgcca
     3901 actgataccg gaactggacg gcgcggtgat cgtgacgacc ccgcagaaag tggcggcagc
     3961 ggacgtgcgc aaatccatca atttctgtaa agaacttggt gtggctgtgc tgggggtggt
     4021 tgaaaacatg agtggtttcg cctgcccgaa atgcggcgag gtcacgccga ttttgagtgc
     4081 ggacggcgga cgcgtgacag ccgatgagat gggcataccg ttcctaggtt cgatcccgat
     4141 cgatccgttg atcgcggaat cgggtgacaa cggcaagcct ttcgtcgaat cggcggcgag
     4201 ttctccggcg gccggtgcct tccgcgagat cgtgagggtg ttgacggtca ctccgacgct
     4261 gtcacaggaa aaaccggcat gtgtttaacc taaaaagagc agttgaactt tcaactgctc
     4321 tttttaggtt tataggttta atgccttacg gtcgatcagg ccaatgaaaa cggattgcgt
     4381 acggcaacgg gatgtgtcac gctaccctat acgttttcat gtcgcggcgg gcgtccggtt
     4441 tacgggtgcg cgtggaaaga gaggatgccc cggtttggcg gggtagggga agggagtttt
     4501 gcgtgaggac gatgacgggg ttgggattgg cgctcttgtt ggtgggcggg gggcagtgtg
     4561 ctgattttta tgtggcgacg aacggggcgg accgcgcgga cgggacacgg gagaagccgt
     4621 tggctacgct ggccgcggca cgcgatgcgg cacggcgttc cggaggcggt ggacaccgga
     4681 tccggttgtt gcccggtgag tattttcttg aagaaccgct cacgctggat gcgcgggaca
     4741 atgggctgac ggtggaagcg gagggggcgg gccgcgtgac ggtatatggc ggccgacgtg
     4801 tcacggggtg gcaccgtgac ggcgagaagt tctggtgtgc ggatctgccg gaggttaaaa
     4861 gcggcgcgtg ggatttccgg gcactggtgg tgaatggccg gatgccggac cgggcgcgga
     4921 tgccggaaag cggcaccttc acccaccaga acgtgttcga tgccaaatgg ctctccagcg
     4981 tgggtggcgg gtgggaacgc aaacccacgc acgccgatct gaccacgctc gtctatcatc
     5041 ccaccaatct ccccccttcg ctggaggtgc gtaacgcgga gttacgcgtg tatcacatgt
     5101 gggatgattc gctggtgggg gtggccagca acgatgtcgc gcggcacacg ttgcatttca
     5161 cctctccggc gggccacccc gcaggagcgt tcggcgtcaa aaaatacgtg gtcttcaaca
     5221 cgcgcgaagg gatgacccgc cccgggcagt ggtat
//
LOCUS       DAOSLE010000172         5243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_14095_length_5243_cov_7.726870, whole genome shotgun sequence.
ACCESSION   DAOSLE010000172 DAOSLE010000000
VERSION     DAOSLE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_5243_cov_7.726870"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2929)
                     /locus_tag="PK576_09590"
     CDS             complement(<1..2929)
                     /locus_tag="PK576_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE61881.1"
                     /translation="MLGIWVKRSLRYYGLRQVLFAATAALTTAILSAALLTGESLRRT
                     LARGVTARLGTIRSVILRDAGPFPATLADRLPDTQAVLLLNGELLTSEGIVCDARAQI
                     LGAGGESADRVDTDRVWMAALNARGRAQMPLSGEAAIRFAKPRVLPAELPLGAARETR
                     MVRRAVRAGPVADEPTRPPEDFALWPGSVPPVNVRVPLEPLAHAAGVPGMANLLLSTL
                     APRDLERALTTVLTPEDAGLTLTPIGDGGLPTTLLKSRAVYLPRALPAVLARAGLCAS
                     EATFHLVDAFEAGERSTPYGFVAGLTPGTGPVPQDLREDEVVLNAWLAEMLGVETNGT
                     LTLRWRRFEAGGRLVEDQRTFRVRAVTGMTEAASVKTRMPVFPGLEGVDRCAAWDVGM
                     PMDEEKLNDAANEAYWDAWRETPKAFLTYETAKRGFGTLFGEAMSVCVSADTDTVSQA
                     LRGLSPVQAGFTIRPVWDEGLRAAQGTTDFRLLFTGMACVLMIAALTLSGLSLALALE
                     TRKHEAALFRAVGWSRSRVAAMLAAEWGVPLVAGTVAGAGLGAGLSRALVWGLGRFWK
                     DAFAGAETVFLFSWPTALSAVGIGLLLTAAVMFRTVTRMTACPPVALWQSADAPPAPA
                     TSVTGRALRENALGAAMAVAALMLMVAARNSGSAAGAFFGAGFLLMFSLLLFFRTACA
                     RLRREEAPVITSPVAAGVVRTLTTPHRSTAVVLLLACGIFLTLGVLSMRQDPAAGCEH
                     TWSGSGGFASIVSSATPLDRERGLALARRVSGSQGVVPLRVREGDEAGCLNMSLPQSP
                     RLIGLDTRAMARARAFEPRDAGGVWSLLDRPLPDGSIPALAADQAMLRYSLKARADPV
                     DGTVYTYTGAGGETWRVRLVGALPVRSGILQGALLVDEHAFMRMFPAEGYRLWLCDYA
                     PYLLREAADRHTASSPKKNVVGEQAVARLRHPEPGVTVETVAERLRLLGSMEHAYLDL
                     FLVL"
     gene            complement(2959..3864)
                     /locus_tag="PK576_09595"
     CDS             complement(2959..3864)
                     /locus_tag="PK576_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOE61882.1"
                     /translation="MMDWRMRAGVVWIAACACAVAAGLDEVVAPGTVAEPVATGFVFT
                     EGATVNAAGEIFFTDQPNNRILRWSREQGVRVFLSPAGRANGMCFRADGTLLVCADEK
                     NELWSVTPDGAVTVLTSTFEGKKLNGPNDVWARPQGGCYFTDPFYKRRWWTHAEAPQA
                     TQQVYFLPPNGAPIRVTEDLVTPNGLVGTADGTTLYVADLGAERTYAYTILPDGRLDR
                     KRLFCQMGSDGMTLDADGRLYLTHKTGVSVFSPAGTLLGVIPIPESWTANVCIGGPKK
                     DTLFVTAMKTLYAVPLRVRGTPQGK"
     gene            complement(4024..>5243)
                     /locus_tag="PK576_09600"
     CDS             complement(4024..>5243)
                     /locus_tag="PK576_09600"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOE61883.1"
                     /translation="FGSGYGGNALLGKKCLALRIASSLARKEGWLAEHMLILKLTSPE
                     KKVYYITAAFPSACGKTNLAMMEPRLPGWTVETIGDDIAWMKIGADGRLYAINPEYGF
                     FGVAPGTSMTSNPNAMRTITRNTIFTNCALTDDGDIWWEGMDGEPPAHAIDWKGNDWT
                     PDMRDKDGNKIKAAHPNARFTAPAAQCPVIADEWEDPNGVPVSAFLFGGRRPTVIPLV
                     NQAKDWTHAVFMGSAAGSETTAANLAKVGVVRRDPMAMLPFFGYNAADYLLHWLTMPL
                     RTDVEKLPKVFFTNWFRKDAKGFMWNGFGDNIRVLKWVCERIDGKGKARETPIGFLPT
                     PDAIDMTGYVDANRDQACMKATMEALLSVDVEGWKKEIAAVAKSYEPFGNRMPFALKA
                     KLAEIQNKLNAAK"
BASE COUNT          962 a         1746 c         1654 g          881 t
ORIGIN      
        1 cgagtaccag aaaaagatcc agatacgcgt gttccatcga acccagtagc cgcaaacgtt
       61 ccgcgacggt ctccaccgtg actccgggtt ccggatggcg cagccgtgcc acggcttgct
      121 ctccgacaac gttttttttc ggagacgatg cggtatgccg gtcggccgct tcacgcagca
      181 gatagggagc gtagtcacat aaccagagac ggtatccttc ggcggggaac atccgcatga
      241 aggcgtgttc gtccaccagc agcgcaccct gcaggatacc gctgcggacc ggcaatgcgc
      301 ccacaaggcg cacgcgccaa gtttcgcctc ctgcccccgt gtaggtatac accgtgccgt
      361 ccaccggatc ggcccgggcc ttcaggctgt accggagcat ggcctgatcc gccgccagcg
      421 cggggatcga tccgtccggc agcggacggt cgaggagcga ccagacgccg cccgcgtcac
      481 ggggttcgaa tgcccgtgcg cgcgccatgg cccgcgtgtc gagaccgatc agacgcggcg
      541 attggggaag actcatgttc aggcagcccg cctcgtcacc ttcccgcaca cggaggggca
      601 caaccccctg actgccgctg acacgccgcg ccaaggcgag gccgcgttcg cggtcgagcg
      661 gcgtggcgga ggagacgatt gacgcgaatc ccccgcttcc agaccaggtg tgttcgcatc
      721 cggccgcagg atcctgacgc atcgagagta caccgagggt caggaagatg ccgcacgcca
      781 gcagaaggac caccgccgtg ctacggtgcg gcgtcgtcag cgtgcgaacc acgccagccg
      841 caacgggaga ggtgatcacc ggcgcctctt cccgccgcag gcgcgcgcag gcggttcgga
      901 agaacagcaa gagcgaaaac atcaggagaa aaccggctcc gaaaaacgcg cctgcggcgg
      961 agccgctatt acgtgcggcg accatcaaca taagcgccgc cacggccatg gccgcaccca
     1021 acgcgttctc gcgcagagcg cggccggtta cggacgtcgc gggtgcgggc ggcgcgtcag
     1081 cactctgcca cagcgcgacc ggtgggcagg ccgtcatccg ggtgacggtc cggaacatta
     1141 ctgcggccgt gaggagcagt ccgatcccaa ccgcgctcag tgccgtaggc caagagaaga
     1201 gaaagaccgt ctccgccccg gcaaaggcat ccttccagaa ccggccaagg ccccacacca
     1261 aagcgcggga aagtccggca ccaagcccgg cgcccgcaac cgttcccgca acaagcggaa
     1321 cgccccattc ggcggccagc attgcggcca cccgcgaacg actccagccg accgcgcgga
     1381 acagggctgc ctcgtgtttg cgggtttcga gcgccaaggc cagagagagt cccgagaggg
     1441 tcagagccgc gatcatcaat acgcatgcca tgccggtaaa aagcaggcgg aaatcggttg
     1501 tcccttgtgc cgcccgcaac ccctcatccc aaaccggccg gatcgtgaat cccgcctgaa
     1561 ccggcgagag cccccggagc gcttgcgaca ccgtatccgt gtcggcagag acgcacacgc
     1621 tcatcgcctc gccgaacagc gtgccgaagc cgcgcttcgc cgtttcgtag gtgagaaacg
     1681 ccttgggggt ttcacgccac gcgtcccagt aagcctcatt ggcggcgtca ttcagttttt
     1741 cctcatccat gggcatgccc acatcccacg ccgcgcaccg atctacgcct tcgagccccg
     1801 gaaaaaccgg catccgcgtt ttgacggacg cggcttcggt catgccggtc accgcacgca
     1861 cacggaatgt ccgttgatcc tccacaagcc gtccgccggc ctcaaaacga cgccagcgca
     1921 gggtcagggt gccgttcgtc tccacaccca gcatttcggc caaccaagca ttaagaacca
     1981 cctcgtcttc gcgcaaatcc tgcggcaccg gtccggttcc cggagtcagg cccgcgacaa
     2041 aaccgtaggg cgttgaccgt tcgccggcct cgaaagcgtc gacaaggtgg aaggtcgctt
     2101 cgctcgcaca caggcccgca cgcgccagca cggcaggcag ggcacgcggc agatacacgg
     2161 cgcggctttt cagcagtgtg gtcggcaagc ccccatcccc tatcggggtg agggtgaggc
     2221 ccgcgtcctc tggggtcaac accgtggtca acgcgcgttc gagatcgcgg ggcgcgagcg
     2281 ttgaaagcag aaggttggcc atgccgggca cgcctgcggc gtgcgcgagc ggttcgagcg
     2341 gcacacgtac gttcacaggg ggtacggaac cgggccagag cgcaaaatcc tcaggggggc
     2401 gcgtgggctc gtccgccaca ggtcccgcac ggacggcgcg ccgaaccatc cgggtttccc
     2461 gtgccgcgcc gagcggcaat tcggcgggca agacccgggg tttcgcgaac cggatggccg
     2521 cttcgcccga aagcggcatc tgcgcgcggc cccgggcgtt gagcgcggcc atccagactc
     2581 ggtccgtgtc gacgcggtcc gcagattcgc cgcccgcgcc gaggatctgc gcgcgtgcgt
     2641 cgcacacgat cccctcggaa gtcagcagct cgccattcag taataggacg gcttgcgtat
     2701 ccggcagacg gtcagcgagt gtggcgggaa agggtccggc atcacgaaga atgaccgaac
     2761 ggatcgttcc gagacgcgcg gtcacgccgc gcgccagtgt gcgccgcaac gattcgccgg
     2821 tgagcagcgc ggcggacaag atggcggtgg tcagggccgc ggtcgcggcg aaaagcacct
     2881 gccgcaagcc gtaataacgc aggcttcttt tgacccagat gcccagcatg ggagtctccg
     2941 aaccgcgccc ctactttctc acttgccctg cggcgtgccc cgaacccgca gcgggaccgc
     3001 gtacagggtt ttcatcgccg tcacaaaaag cgtgtctttt ttcgggccgc cgatgcagac
     3061 gttggcggtc caggattccg gaatcggaat cacgcccagc agggttccgg cgggactgaa
     3121 gacgctgacg cccgttttat gggtcagata aagccgaccg tcggcatcca gggtcatgcc
     3181 gtctgaaccc atctggcaaa agaggcgttt gcggtcgaga cgcccgtccg gcaggatggt
     3241 ataggcatac gtccgttcag cgccgaggtc cgccacataa agcgtggtgc cgtcggccgt
     3301 gccgaccagt ccgttggggg tgaccaggtc ttccgtcaca cggatgggcg cgccgttcgg
     3361 cggcagaaag taaacctgct gtgtggcttg gggcgcctcg gcatgggtcc accagcggcg
     3421 tttgtagaag gggtccgtga aataacagcc gccctgcggc cgcgcccaga cgtcattcgg
     3481 gccgttcagt ttttttccct caaaggtgct ggtcaggacg gtgaccgcac cgtccggcgt
     3541 gacggaccac agttcgttct tctcgtcggc gcagaccagc agcgtgccgt ccgcacggaa
     3601 gcacatgccg ttggcgcggc cggccggact caggaaaacc cgtaccccct gttcgcgcga
     3661 ccagcgcaga atccggttgt tcggctggtc cgtgaagaaa atctctcctg cggcattgac
     3721 ggtcgccccc tcggtgaaga cgaagccggt tgccaccggt tcggctaccg ttccgggcgc
     3781 caccacctca tccagtccgg ccgccaccgc gcacgcgcaa gccgcgatcc aaaccactcc
     3841 cgcccgcatt ctccaatcca tcatcggtta cccccttccc ggtttcccgg tttttctgtt
     3901 ttcctgtctg acgggacgca gtgtacagtt ttcggaccgg taacacaaac agcgattgtt
     3961 ttcggaaaga atgcaaaacg gggcccggga cattgccgtc ccgagccccg ttggatgaaa
     4021 cggttatttc gccgcgttca gtttgttctg gatctcggcc agcttcgcct tgagcgcgaa
     4081 cggcatccgg ttgccgaacg gctcatacga tttcgccact gcggcgatct cttttttcca
     4141 cccttcgaca tccacagaga gcagggcttc catcgttgct ttcatgcagg cctgatcccg
     4201 gttcgcgtca acgtaaccgg tcatgtcgat ggcgtcgggc gtgggcagga acccgatggg
     4261 ggtctcgcgc gccttcccct tgccgtcgat ccgctcacaa acccacttca gcacgcggat
     4321 gttgtccccg aagccgttcc acatgaaccc tttcgcatcc ttgcggaacc agttcgtgaa
     4381 aaacaccttg ggtagctttt ccacatcggt gcgcaaaggc atggtgagcc agtgcagcag
     4441 ataatccgcc gcattgtagc cgaagaacgg cagcatcgcc atggggtcac gacgaactac
     4501 gccgaccttg gcgaggttgg cggcggtggt ttcggaaccg gcggcggaac ccatgaacac
     4561 cgcatgcgtc cagtccttgg cctggttcac cagcgggatc acggtcgggc ggcggccgcc
     4621 gaacaggaag gcggaaaccg gcacgccgtt gggatcttcc cactcgtcgg cgatcaccgg
     4681 gcactgcgcg gccggcgcgg tgaagcgcgc gttcggatgg gcggccttga ttttgttgcc
     4741 gtccttgtcg cgcatgtcgg gcgtccagtc gttacccttc cagtcgatcg cgtgggccgg
     4801 cggctcgccg tccatcccct cccaccagat gtcgccatcg tcggtcaagg cgcagttggt
     4861 gaagatggtg ttccgggtga tggtccgcat cgcattcggg ttactggtca tcgacgtgcc
     4921 gggcgccaca ccgaagaacc cgtattcggg gttgatggcg tacagacggc cgtccgcgcc
     4981 gatcttcatc caggcgatat cgtcgccgat ggtttcgacc gtccaaccgg gaagacgggg
     5041 ctccatcatc gcgaggttgg ttttgccgca ggcgctcggg aaggccgccg tgatataata
     5101 aactttcttc tcgggtgacg tgagcttgag gatcagcatg tgctcggcca gccagccctc
     5161 cttgcgcgcg aggctggagg cgatgcgtag cgccaggcac ttcttgccga gcagggcgtt
     5221 gccgccgtaa cccgaaccga acg
//
LOCUS       DAOSLE010000173         5231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_14132_length_5231_cov_8.393354, whole genome shotgun sequence.
ACCESSION   DAOSLE010000173 DAOSLE010000000
VERSION     DAOSLE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14132_length_5231_cov_8.393354"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1596
                     /locus_tag="PK576_09605"
     CDS             160..1596
                     /locus_tag="PK576_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61884.1"
                     /translation="MKRIKMVVLGITLFSALSVCAAGTFRAGAAKGNITPELGGKIVG
                     GFSPFPSTHVHDDLWVRVVVLDDGKTRVALAVCDLIGISQDVSDKARQLVQEETGLPA
                     RNLLVAATHTHSATSARGNSDFSEKNPEMDAYQKFVTRRIADTVRCAVNNLAPAQIGW
                     GTASEPRHVFNRRWHMKPGTNPMNPLGGTNDIVRFNPGHLNPNLVKPAGPTDPEICFF
                     ALQTPEGKPVALFANYSLHYVGGVRGADISADYFGMFNDRIQQLLNADRQSPSFISIL
                     SNGTSGDINNIDFSKKHEKMPPYAKMRIVAHDVAEKVHAAYRELKWHDHVTVGAAFEE
                     IELQLRKPTPEQVAYAKAFFAEQKENVKPDPWRQSYANRIMQHDRSPATWKFPLQAFR
                     IGDVGISCIPFEVFCEIGLELKQRVPFKPSFTVELANGTYGYLPNPRQHALGGYETWL
                     GSNRVEIEASTKIVDRLLEMFTRLQKGE"
     gene            complement(1713..5168)
                     /locus_tag="PK576_09610"
     CDS             complement(1713..5168)
                     /locus_tag="PK576_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61885.1"
                     /translation="MSACDCFNAVLRGKETVKPWVARAAAIFVGAAAAMTLDVNAAPM
                     PKVGVTLAFRGGYGSAQYVSNASFKGRPVVEDFAKQGLDLCYYAGWRGAKTPLASLKQ
                     FNAVWLVIDHEDQCPYPAEETIAALKEYVSAGGGLVVTHSGGRYSEAPVDAYWTKVYA
                     AFGLDLLHEEIVDDATSARVDRHFDLFRVEDFADHPIAEGIPGLWLPIRRSMGSWGVT
                     AFRTSPEWQWIARTSPTARSVPKGKIDNRPKWDQPGTFAKGPIPVVAARAFGKGRVVF
                     IAIHKDNVGWAYNIDRWPNWVERSVFNDKRSDALGLLENACAWTAEPSLTDASFTADY
                     KPVEPDYPPYTRHDVGRHENEKPVWKARPVADRKPGVVGLIGLHSSYSDGASTVAEYV
                     AEAKRLGLSFLVFTDPIARLTEDTLGKLRADCAANSSEEFYCCPGVEFREPSGYEWML
                     FSDKVSWPQESVHRDGRDYVVFDGKNMLQPGNYRGQNLYRGCLLNLGEIPGKGSEYGS
                     LHWMNEVVAVGYGMDADGSRVLYNNAPAYRELPRYLLRCATVSYTRVKRAADLARAKD
                     MSVTCAESLAGVRKMANAQGHGANRAAVAAHVWAAYGTDAEIRDFTHDRILGTDTLRF
                     TVRAVSPTGLKALTVHDATLRVLARFDAKGAKAFEKTFTVTSDRQMYPQLVVEDVKGG
                     KAVSTMREVNYPHAGLHRCGDNFNLLSYNPHVVMHPNWDTQLCPPYKFILMERKHFQI
                     SEATFWEAFERAPSREPSVRIQPQWNTLRLENVPFPSEQKHLMASSRTEYTLVAPNVV
                     AIFDQTQGERLVEPTRSDTHGTFSNCPLAIQVGENEWWRHRYRVYQFTDRMDAWWMAV
                     YHQVSPDYRGGYTVVEGALKFLRDATITEPLTLVRLRTHNPKGTVEVFRRNVENFGPG
                     SYHASCGSPAAWHAFFGLKDSVPLVFRESKLPDGVETRVMLTESGRRVKAGEQIRYRF
                     ALGSFCEKPHGGMYCDWFASMLDGSGFNHEAVVGRVTDVNGMVEVAAEGNVAKVTFGP
                     TWFIQDYPVRISGLVDNGTAYVMDGAGRLVRPLAFENGRAYAEIPLEKRQTWTFLNLF
                     VADDPALRLSYVPAMPGHEKPTLQILNPTDRDISTRVTDVRTRKTFTVSVPASGCADD
                     VFGAQ"
BASE COUNT         1070 a         1646 c         1542 g          973 t
ORIGIN      
        1 cttaccgata acttcaatca gaaatcctgt taatcctgta atcctggctt aaagtttaac
       61 gttcccgtca ccaccgcgct tgcccgaaat gttttcgggc ttgacggctt ttgcccccca
      121 tcgcacaata gtttctaact acaaaagaca aggaatcgta tgaaacggat aaagatggtg
      181 gtgttgggca ttacactgtt ttccgcgttg agtgtctgcg cggcgggcac gttccgcgcg
      241 ggggcggcaa agggcaacat cacgccggag cttggcggaa agattgtcgg cggattcagt
      301 ccgttcccgt ccacgcacgt gcatgacgat ttgtgggtgc gcgtcgtggt tctggatgac
      361 gggaaaacac gtgtggccct agccgtgtgc gacctgatcg gcatctcgca agacgtgagc
      421 gacaaagccc ggcaactggt gcaagaggag acgggactgc cagccagaaa ccttctcgtt
      481 gccgcgacgc acacccactc ggcgaccagc gcccggggga acagtgattt ttcagaaaag
      541 aatcctgaaa tggacgcgta ccagaagttt gtgacccggc gcatcgcgga cacggtgcgc
      601 tgcgccgtca acaacctcgc gcccgcccag atcggctggg gcaccgccag cgagccgcgc
      661 cacgtcttta accgccgctg gcacatgaaa cccggcacca atccaatgaa cccgctcggc
      721 ggaacgaatg atatcgtgcg tttcaacccc ggacacctca acccgaactt ggtcaaaccc
      781 gccgggccca ccgatcccga aatctgcttc ttcgccttgc agaccccgga aggcaagccg
      841 gtcgcgctgt tcgcgaacta ctccctgcac tatgtgggcg gcgtgcgcgg cgcagacatc
      901 tcggcagact acttcgggat gttcaacgac cgcatccagc agctccttaa tgcagaccgg
      961 cagtccccct ccttcatctc tatcctcagt aacgggacca gcggtgacat caacaacatc
     1021 gacttctcca aaaaacatga gaaaatgccc ccgtacgcaa aaatgcggat cgtggctcat
     1081 gacgtggcgg aaaaggtgca tgccgcttac cgcgaactca agtggcacga ccatgtaacc
     1141 gtcggcgccg cgttcgagga gatcgagctc caattgcgca agcccacgcc cgaacaagtt
     1201 gcgtatgcca aagccttctt tgccgaacaa aaggagaatg tcaaaccaga cccgtggcgg
     1261 caaagttacg cgaaccggat catgcagcat gaccgttctc ccgcgacgtg gaagttcccc
     1321 ttgcaggcct tccggatcgg cgatgtcgga atctcatgca taccgttcga ggtcttctgc
     1381 gagatcggct tagagttaaa gcagcgggtg ccgttcaaac cgtcgttcac cgtggaactc
     1441 gcgaacggga cttatggcta cctgccgaat cccagacaac acgcgctggg cggttatgag
     1501 acctggctcg gctccaaccg ggtcgagatc gaagcgtcaa ccaagatcgt cgaccgcctg
     1561 ttggagatgt tcacacgcct gcaaaaaggg gagtgacgca agttgcaggc tgcgagccag
     1621 cagcgtttga accttgaaac acaagaaact tcatggaaat cagggacacc cctcggcgtc
     1681 ttggcggttt gctcaaggga tccacgagcg cgctactgtg cgccgaaaac atcgtccgcg
     1741 cagccgctgg cgggaaccga taccgtgaac gtcttgcgcg tccgcacgtc cgtcacgcgt
     1801 gtggagatat cgcggtcagt cgggttgaga atctgtaacg tgggcttctc atgtcccggc
     1861 atcgcgggca cgtaggagag ccgcaaagcc ggatcgtccg cgacaaacag gttgagaaac
     1921 gtccaagttt ggcgcttctc cagcggaatc tcggcatagg cgcggccatt ctcgaatgca
     1981 agcggacgga ccagacggcc ggccccgtcc atcacgtagg ccgtaccgtt gtcgacgagc
     2041 cccgaaatgc ggacggggta gtcctgtatg aaccaagtcg gcccgaacgt taccttcgcg
     2101 acattgccct cggccgcaac ctcgaccatg ccgttcacgt ccgtcacgcg gcccacaaca
     2161 gcctcgtggt tgaagcccga tccgtccagc atggaggcga accagtcgca atacatcccc
     2221 ccgtgcggtt tttcacagaa cgaacccagc gcaaaacggt agcgtatctg ctcgccagcc
     2281 ttcacccgac gtcccgactc cgttaacatg actcgcgtct cgacgccgtc cggcaacttg
     2341 ctctcgcgga aaacgagcgg cacggaatcc ttgagtccga aaaatgcgtg ccaggccgcc
     2401 gggctgccgc atgaggcatg gtaggagccc ggaccaaagt tctcgacatt gcgtcggaac
     2461 acctcgaccg ttccctttgg attgtgggtc ctgaggcgca caagcgtcag cggctcggtg
     2521 atcgtggcgt cccgcaggaa cttgagcgcg ccctcgacga ccgtgtagcc accgcgatag
     2581 tcaggcgaca cctgatgata gacagccatc caccaggcgt ccatgcggtc tgtgaactgg
     2641 taaacgcgat accgatgacg ccaccactcg ttctcaccga cctgaatggc gagcggacag
     2701 ttgctgaacg tgccgtgggt atcgctgcgc gtcggctcga cgagccgctc gccctgcgtc
     2761 tggtcgaaga tcgcgacaac gttcggcgcg acaagcgtgt actccgtgcg cgacgatgcc
     2821 atcaggtgct tctgctcgct cgggaacggc acgttctcga gccgcagcgt attccactga
     2881 ggctggatgc gcacagaggg ttcgcggctc ggcgccctct cgaacgcctc ccagaaggtg
     2941 gcctccgaaa tctgaaaatg tttcctctcc atcaagatga acttgtacgg cgggcagagt
     3001 tgggtgtccc aattcgggtg catgacaacg tgaggattgt acgagaggag attgaagttg
     3061 tcgccgcagc ggtggagtcc cgcgtgcgga tagttcacct cgcgcatcgt ggagacggcc
     3121 ttgcccccct tgacgtcctc gacgacgagc tgcggataca tctgccggtc cgaagtgacg
     3181 gtaaaggtct tttcgaatgc ctttgcaccc tttgcatcga agcgcgcgag aacacggagc
     3241 gtggcgtcat ggacggtcag cgccttgagt cccgtcggcg agacggcacg taccgtgaag
     3301 cgcagcgtgt ccgtgcccag gatgcggtcg tgcgtgaaat cgcggatctc cgcgtcagta
     3361 ccgtacgccg cccagacgtg cgcggcgact gcggcccggt tcgcgccatg accttgggcg
     3421 ttcgccattt tccggacacc ggcgagcgat tcggcacagg tcacagacat gtccttcgcc
     3481 cgggcgagat cggccgcccg ttttacgcgc gtgtaggaga cggtcgcaca gcgcagcagg
     3541 tagcgcggca actcgcgata ggcaggggca ttgttgtaga ggacacggct gccgtccgca
     3601 tccatcccgt acccaacggc gacgacttcg ttcatccagt ggaggctgcc gtattcggag
     3661 cctttccctg ggatttcgcc cagattcagg aggcaaccgc ggtagaggtt ctgcccgcgg
     3721 tagttgccgg gctggagcat gtttttccca tcgaagacga catagtcccg cccgtcgcga
     3781 tggacggact cctgcggcca cgaaaccttg tcgctgaaaa gcatccattc ataacctgac
     3841 ggctcccgga actcgacgcc cgggcaacag tagaactcct cgctcgagtt cgccgcgcaa
     3901 tccgcgcgca acttcccgag cgtatcttcc gtcaaccggg cgatcggatc ggtaaagaca
     3961 aggaacgaaa gccccagacg tttcgcctcc gcgacgtact ccgcgaccgt cgacgcgccg
     4021 tccgaatagg acgagtgaag cccgatgagc ccgacgacgc cgggcttgcg atccgcaacc
     4081 gggcgcgcct tccagacagg cttctcattt tcgtggcggc caacgtcgtg ccgcgtgtac
     4141 gggggataat ccggttcgac gggcttgtag tccgccgtga aggaagcatc ggtgagcgac
     4201 ggttcggctg tccacgcgca ggcgttttcg agaagcccga gcgcatccga cctcttgtcg
     4261 ttgaacacac tgcgctcgac ccagttcggc cagcggtcga tgttgtacgc ccagccgacg
     4321 ttgtccttat ggatggcgat gaacacgaca cggcccttcc cgaacgcgcg cgccgcgacg
     4381 acagggatcg gccccttcgc gaaagtgccg ggctgatccc atttcgggcg gttatcgatc
     4441 ttgcctttcg gaacactgcg ggcggtcggc gacgtccgcg cgatccactg ccattcgggt
     4501 gacgtcctga acgctgtcac gccccacgac cccatactgc ggcggatcgg cagccacagt
     4561 ccggggatgc cctcggcaat gggatgatcg gcgaaatcct cgacgcggaa gaggtcgaaa
     4621 tgccggtcca cgcgtgcgct tgtggcatcg tcaacgattt cctcgtgcag aagatcgagc
     4681 ccgaacgcgg cgtagacctt tgtccaatac gcatccaccg gtgcctcgga atagcgtccg
     4741 ccggaatgcg tgacaacaag cccgccgcca gcggagacat actccttcag cgcggcgatc
     4801 gtctcttcag cgggataggg gcactggtct tcgtggtcga tgacgagcca gaccgcgttg
     4861 aactgtttca gcgacgcgag cggggtcttc gcaccccgcc agccagcgta gtagcagagg
     4921 tcgaggccct gcttcgcgaa gtcctcgaca acggggcgtc ccttgaagga cgcgttcgag
     4981 acatactgcg ccgagccgta ccccccgcga aaggcgagcg tcacgccaac cttcggcatc
     5041 ggtgcggcgt tcacgtccag cgtcatcgct gcagcggccc caacaaagat cgccgcagcc
     5101 cgagcgaccc acggcttcac cgtctcttta ccacgcaata cagcattaaa gcaatcacac
     5161 gctgacatca tcgtttgctt ttcatgaagt ttccgtttca tggtttcatc caaatcgggg
     5221 tcgggatcgg g
//
LOCUS       DAOSLE010000174         5190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_14279_length_5190_cov_6.574878, whole genome shotgun sequence.
ACCESSION   DAOSLE010000174 DAOSLE010000000
VERSION     DAOSLE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14279_length_5190_cov_6.574878"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="PK576_09615"
     CDS             complement(<1..1550)
                     /locus_tag="PK576_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61886.1"
                     /translation="MVVCTCGVFGGNIEVTNIPDTAVLTQVGEYDLLTFTNNGSFTVT
                     SPGEVDVLVVAGGGGGGSHSGGGGGGGGVFYITNHLVSCGDYTVCVGTGGVSDVNGGD
                     SSIFGWTAVGGGKGGGVAAAGGNGGSGGGGGAGNGGVYNIKRAFPGGTGTDGQGYNGG
                     ASTNNNTASSFTSPAVGGGGGGGGGPGSDATVTLVISEDFATTTTVYKCGNGGNGFAC
                     AITGLEKYYGGGGGGGTGNGNSSVAGSGGLGGGGAGAYSTRTGGAGENLTGGGGGGGG
                     RNANTGYPGGPGGSGVVMLRWKNTSRADVSTGKVTAWGGTAVWDDGYISRSFVLDGQL
                     TFSEETEVELLMVAGGGGGSNGSRGGGGGAGGVIYTNALMVPAGTYNIIVGRGGGAGT
                     NGENSVALGFTAVGGGHGGSANVGGAAGGSGGGGGARNNGTTSPGGACTEGQGNIGGT
                     GYNNGGAGGGGGIGSPGGNVTSGNTINSGAGGTGMLYRVSGHAFYYAGGGNGTQNRTW
                     VPGGGGRG"
     gene            1825..2694
                     /gene="speB"
                     /locus_tag="PK576_09620"
     CDS             1825..2694
                     /gene="speB"
                     /locus_tag="PK576_09620"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOE61887.1"
                     /translation="MKNAFLASECPPVPADDAAFHVIPAPMELSVSYGQGTRLGPSAI
                     LAASQQLEACDAEGRLTPARIHTQRAVSSRSRDPEVWIAAVEKRVAQTLACRSVPVLL
                     GGEHTVSLGAARAFHAARDLVGFIQFDAHADLRGTYEGSPLSHACVMRRIHELGFPCL
                     QIATRAVSPEEQEYRKAHRSSISFYDARVIADDGMSRHFIPRGFPKRVYITFDLDGLD
                     PAVLPATGTPVPGGLFWYDAIRILRRIAATRDIVGLDVVELAPNRASPHADFTAALLT
                     QTLMGFAAASPTR"
     gene            2710..4521
                     /locus_tag="PK576_09625"
     CDS             2710..4521
                     /locus_tag="PK576_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE61888.1"
                     /translation="MALISLRKVSVQYGGPAVLDQISLSIEPGERACVTGRNGEGKST
                     LLKLLAGLIVPDTGERIIQPDLRVAYLSQDVPGDLDGTVAEIAGSGVVDNGACHAHHP
                     GAAMYLSQLKLDGAEQFNTLSGGLRRRALLARTLAAEPHLLLLDEPTNHLDIETIEWI
                     ETFLQRSPCACLFVTHDRAFLKRVATRVLDLDRGRLIGWDCDYDTFLRRKQEQLDDEA
                     VYWSRQTRKLEQEEAWLRRGVKARTTRNEGRVAALLQLRETFRQRRTAPGVSRFQLDL
                     ETSSGEQVLKVSDVSFAYPGGAPVVCGFSAKILRGERIGIIGPNGSGKTTLLNLLCGH
                     LTPDSGYVQPGARVEIALFDQLRSALDLDRTVQQNLAEDREEVMVGCVRKHVFGYLSD
                     FLFTPDRARTPVRALSGGERARLMLAKLFLQPGNLLVMDEPTNDLDMETLELLEEQLM
                     NYDGTLLLVSHDRTFLDNVVTGTFVLDGSGKVELYPGGYSDWLRQRPAPSTLPAVEPD
                     EASVALPARTPPSERRRERLTFKQQRDRQMLPQQIESLETEIASLHAVLSDPEHYRRE
                     PDATREATGRLAQAEAELEAAFKQWVELETLAERLEK"
     gene            4652..>5190
                     /locus_tag="PK576_09630"
     CDS             4652..>5190
                     /locus_tag="PK576_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOE61889.1"
                     /translation="MNRHPDTLCVQEGWQPKNGEPRVLPIIQSTTFKYDSSEHVGDLF
                     DLKVPGHFYTRLSNPTTEAVENKLAALEGGVGALMTAAGQSASLIAILNLCNSGDHFI
                     ASSAIYGGTTNLFSVTLKKLGIEVTYVDSHATDEEIRAAFRPTTRAVFGETIANPALT
                     VFDIERFAHVAHANGVPLIV"
BASE COUNT          922 a         1826 c         1464 g          978 t
ORIGIN      
        1 atcccgcgtc cgccgcctcc cggcacccat gtgcggttct gcgtgccgtt gccgcccccc
       61 gcgtagtaaa aggcatggcc ggaaacacgg tatagcatgc ccgttccacc tgcgccgctg
      121 ttgattgtat ttccgcttgt gacattgcct cccgggcttc ctatgccgcc accgccgccc
      181 gcaccgccgt tgttgtatcc cgtgccgccg atattccctt gtccctccgt acacgcaccg
      241 cccggggatg tcgtgccgtt gttgcgcgcg ccgccgcccc ctccggaacc gccggctgca
      301 ccgccaacgt tcgcactgcc gccgtggccg ccgccgaccg ccgtgaaccc caacgcgacc
      361 gagttttcac cgttggtgcc tgctccgcca cctctgccga cgatgatgtt ataggtgcct
      421 gcaggaacca tcagcgcgtt cgtgtaaata acgccgcccg cgcctcctcc gccgccgcgc
      481 gaaccgttcg atcctcctcc gccgcctgcg accatgagga gttccacttc cgtttcctcc
      541 gagaacgtga gctgtccatc taaaacgaac gacctcgaga tgtatccgtc gtcccacacc
      601 gcagtaccgc cccaggccgt caccttcccc gtcgacacat cggcgcgcga ggtgttcttc
      661 caacgcaaca tcacgacccc cgaaccgccc ggaccgccag ggtatcccgt gttggcgttc
      721 cgtccgccgc cgcctcctcc gccgccggtc aggttctcac ccgctccgcc ggttctcgtg
      781 gaatatgcgc cggcaccgcc gccgcccaaa ccgccggacc cggcgacgga agaattgccg
      841 ttaccggttc cgccgccgcc gccgccgcca taatattttt cgaggccggt tattgcacag
      901 gcgaacccgt tgccgccgtt cccgcacttg tagacagtgg tcgtcgtcgc gaagtcctcg
      961 ctgatgacga gggtaacggt agcgtcgctg cccggaccgc cgccgccacc cccgccgcca
     1021 ccgaccgcag ggctggtaaa cgatgacgct gtgttgttgt tcgtgcttgc gccgccgttg
     1081 tatccctgcc cgtcagtgcc ggttccgcct ggaaatgctc ttttgatgtt gtagacgccc
     1141 ccgttaccgg cgccgccgcc accgccggaa ccgccgttgc caccggccgc cgcgacaccc
     1201 ccgcctttac cgccgccgac cgccgtccac ccgaaaatcg aggagtcgcc gccgttgacg
     1261 tcacttacac cgcctgtacc cacacacacg gtgtagtcgc cgcaagaaac cagatgattc
     1321 gttatgtaaa aaacgccccc tccgccacca ccgccgcccg aatgcgaacc gccgccaccg
     1381 ccccccgcga ccacgaggac atccacctcg cccgggctcg tcaccgtaaa cgacccgttg
     1441 ttcgtgaacg tgaggaggtc gtattcacca acctgcgtca gcacggccgt atccgggata
     1501 ttcgtaactt cgatgttgcc gccgaacaca ccgcacgtac atacaaccat cattgttccg
     1561 cacacaattg ctttcttcat catgcgcctc tctggtgttc ctttgatgct ttcggtgaca
     1621 tgccgaagca caaaacatcg taataaagaa tattcgcaca ttccgaggcg tttgtctatc
     1681 ccgccctttt tgaaaaatcg tttcatggta ttgttttgca acatgttaca cattaaaatc
     1741 actggctgtt gacgtccggg gcttgcatcc cgttccgtac cccggtatgc ttccgttttt
     1801 cgtattcccc acccttctca cattatgaag aatgcttttc tcgcttccga atgtcccccg
     1861 gtcccggccg atgacgccgc ctttcatgtg atccccgcac cgatggaact cagcgtctcg
     1921 tacgggcagg gcacccgact gggcccgtcc gcaattttgg cagcctcgca acaactcgaa
     1981 gcctgcgacg ccgaaggacg cctcaccccc gcgcgtatcc atacacagcg tgcggtgtcc
     2041 tcgcgcagcc gcgacccgga ggtctggatc gcggcggttg agaagcgggt ggcgcagacc
     2101 ctcgcctgcc ggtccgttcc ggttctgctc gggggcgagc acacggtgtc gctcggcgcg
     2161 gcgcgcgctt tccacgcggc gcgcgatctc gtcgggttca tccagttcga cgcgcacgcc
     2221 gacctgcgcg gcacatatga gggttcgccg ctgtcgcatg cctgcgtgat gcggcgcatt
     2281 cacgagttgg ggttcccctg cctgcagatc gccacccgcg cggtctcacc cgaagagcag
     2341 gagtaccgga aggcccatcg ttcctcgatc tcgttttatg acgcgcgtgt catcgcggac
     2401 gacggcatga gccgtcactt cattccgcgc ggttttccga aacgggttta tatcaccttt
     2461 gatctggacg ggcttgaccc cgccgtgctg ccagccaccg gcacaccggt acccggcggg
     2521 cttttctggt atgacgcgat ccgcatcctg cgccgcatcg ctgccacgcg tgatatcgtg
     2581 ggtctggatg tggtcgaact cgcaccgaac cgcgcgagcc cgcatgctga tttcactgcc
     2641 gcgctgttga cgcaaaccct gatggggttc gccgcggcat cccccacgcg ttaaccccgg
     2701 aaccgctcga tggccctgat ttccctcaga aaggtgagtg tccaatacgg cggaccggcc
     2761 gtattggacc agatcagcct cagcatcgaa ccgggtgaac gcgcctgtgt caccggtcgt
     2821 aacggcgaag gcaaatcgac cctgctgaag ctgctcgcgg gcctgatcgt cccggacact
     2881 ggggaaagaa tcatccagcc cgacctgcgg gtggcctacc tctcccaaga tgtgccgggc
     2941 gatctggacg gcacggtggc cgaaatcgcc gggtctggag tcgtcgacaa cggggcttgc
     3001 catgcccatc atcccggtgc ggcgatgtac ctttcccaac tgaagttgga cggcgcggag
     3061 caattcaaca cgctctcggg cggcctgcga cggcgcgcac tgctcgcacg gacactcgct
     3121 gcggagccgc acctgctatt actcgacgag ccaaccaacc atctcgacat cgagacgatc
     3181 gagtggatag aaaccttctt acagcgcagc ccctgcgcct gcctcttcgt gacgcatgac
     3241 cgcgccttcc tgaagcgcgt cgccacgcgg gtgttggatc tcgaccgcgg ccgactgata
     3301 ggatgggatt gcgattacga cactttcctg cggcggaaac aggaacagct cgatgacgag
     3361 gccgtgtact ggtcgcgcca gacccgtaaa ctcgaacagg aggaggcttg gctccggcgc
     3421 ggggtcaaag cccgcacaac ccgcaatgag ggacgcgtgg ccgcactgct ccagttacgc
     3481 gaaaccttcc gtcagcgccg taccgcacca ggcgtcagcc ggttccagct tgatctggag
     3541 acctcttcag gcgaacaggt gctcaaggtt tcagatgtct ccttcgccta tcccggaggg
     3601 gcgccggtgg tctgcgggtt ctccgccaag attctccgcg gcgaacggat cgggatcatc
     3661 ggccccaacg gatcggggaa aaccaccctg ctcaacctgc tgtgcggtca cctcactccg
     3721 gattctggct acgtccaacc cggcgcgcgg gtggaaatcg ccctgttcga ccagctccgc
     3781 agcgctctcg atctcgaccg gaccgtccag cagaatctgg cggaagaccg cgaggaggtg
     3841 atggtcggct gtgtgcgtaa acacgtgttc ggttacctca gtgattttct cttcaccccg
     3901 gatcgggcgc ggacgcccgt gcgggcgctc tccggcgggg aacgcgcccg gctgatgctg
     3961 gccaaacttt ttctgcaacc cggcaacctg ctggtcatgg acgagccgac caacgatctg
     4021 gacatggaga cgctggagtt gctggaagag cagttgatga attatgacgg cacgctcctg
     4081 ttggtcagcc atgatcggac ttttctcgat aatgtggtca cgggcacctt cgtgctggac
     4141 gggagcggca aggtcgaact ctatcccggc ggatactccg attggctgcg ccaacgtccc
     4201 gcgccgtcaa ccctcccggc cgtggaaccg gacgaggcgt cggtcgcgct cccggcacgc
     4261 acaccgccct cggaacgccg acgcgaacgc ctcaccttta aacaacagcg cgatcggcag
     4321 atgctccccc aacagatcga gtcgctggaa accgagatcg cctcgctgca cgccgtcctg
     4381 agcgacccgg aacactaccg gcgggagccg gacgcgaccc gcgaggccac cggccgcctc
     4441 gcgcaggccg aggctgaact ggaagccgca ttcaaacagt gggtggaact cgaaaccctt
     4501 gccgaacggt tggaaaaatg aaaccgtgat cgccgcacgc catccttgtc cggggtgggc
     4561 cgctgtgtta tcatttgctg aatgattgcc gaatgtttcg gccctctgac cccggcccat
     4621 ttgaccggtg ccgcgatgac aaggagaagc aatgaaccga caccccgaca ccctttgcgt
     4681 gcaggagggc tggcagccga aaaacggcga accgcgtgtg ctgcccatca ttcagagcac
     4741 gaccttcaag tacgacagca gcgagcacgt cggcgacctc tttgacctga aggtgcccgg
     4801 ccacttctac acccggctca gcaatcccac aaccgaggcg gtcgagaaca agctcgccgc
     4861 gctggaaggc ggcgtcggcg cgctgatgac cgcggcgggc cagtccgcat cgctgatcgc
     4921 cattctcaac ctctgcaaca gcggtgacca cttcatcgcc tcgagtgcca tctacggcgg
     4981 caccaccaac ctcttcagcg tcacactcaa gaaactgggt atcgaggtca cctatgtaga
     5041 ttcccatgcc accgacgagg agatccgggc cgccttccgt cccaccaccc gcgcggtctt
     5101 cggcgagact atcgccaacc ccgcgctgac ggtcttcgac atcgagcgct ttgcgcacgt
     5161 ggcgcatgcc aacggggtgc ccctgatcgt
//
LOCUS       DAOSLE010000175         5068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_14742_length_5068_cov_6.862358, whole genome shotgun sequence.
ACCESSION   DAOSLE010000175 DAOSLE010000000
VERSION     DAOSLE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_5068_cov_6.862358"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..805)
                     /locus_tag="PK576_09635"
     CDS             complement(185..805)
                     /locus_tag="PK576_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE61890.1"
                     /translation="MSLYLVQGAVYGFAAAVQPGPFLAYLVSLALSRGWRRALPAVLA
                     PLLSDGPIIVLVLIVLSRVPPWWIQLLRFAGGFFILYLAAGALRFWRHPETVAPSDRS
                     DRKGVFRAVLLNLLNPSPYVYWGLVGGPLLLSGWRETPVNGIGFLAGFYGAMVASMAG
                     IILLCACAGRRDERLNRLLSGVSGFALAGFGLYQIALGITNWRWPG"
     gene            1075..2715
                     /locus_tag="PK576_09640"
     CDS             1075..2715
                     /locus_tag="PK576_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61891.1"
                     /translation="MRIGIVWGMVIGWGTVLAAEPCLEYVYPAGVCADSACEVEIGGT
                     HLGNVTQAVISGEGVKAFFLGPVRTVTRNQKGREVPVVLPDRYRFRVVADPEAAPGIR
                     ALRVATAHHLSEPVRFEVGTMSESSEPATLRTETGEVVLSNLPACLNGRCHGRGADVY
                     RFRAAPGSAVVAFVESRTVSANGFVPALTFTDAAGKPCANMKAYDGEDAPVLVLEVRE
                     EGEYRLLVAAGSDAEGGDGCVYRIKLGALPLVTGLTPDRAREGESLNVRLAGHNLARS
                     RLRLFTGGKDSARCLATLTEGALALPSLRFDLANEAQAPGFEATLTPASLNIPPDGSA
                     LVRVEVRRRNGFEGAVRVELDFPPLSIASQGGLIPAGESFCLMTVSTDGARYPRTVFS
                     LPLTATAEINGQPVKRPVVPVRQREGNTAARPQSFDESAARVNPTLYALHLNVAPRKP
                     LSLSTGKPMELMMISSTLAARLGNGCKTVVVWPERGITVQDVQRTNRQERARVMLRTD
                     PSVLQAGASGYLILGCVEKKAPKGPVMAVTQSVPFTVK"
     gene            complement(2702..3544)
                     /gene="mutM"
                     /locus_tag="PK576_09645"
     CDS             complement(2702..3544)
                     /gene="mutM"
                     /locus_tag="PK576_09645"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOE61892.1"
                     /translation="MPELPEVETVVRELRAQGMVGAVIREVRFRWPRIAAGRSPASLA
                     RALRGRTVTSVTRRAKYIVFTLDTGDRLLIHLRMTGKLRFLGAEERPGRHDHALLLLE
                     DSRRLVFNDTRKFGRLRLCGADTDPFATLGPEPLEPAFTTAVLRARLAGKRGKIKPLL
                     LNQTVVAGLGNIYADESLWHAGIHPERRADSLTETEIQRLCRAIRTILRRAITKGGTT
                     LGGGETNFYSVAGRRGRNADDLRVFRRDGLPCPRCGRPIARSVVGQRGTHFCPVCQRP
                     PTSR"
     gene            complement(3592..4773)
                     /locus_tag="PK576_09650"
     CDS             complement(3592..4773)
                     /locus_tag="PK576_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE61893.1"
                     /translation="MKYETTHPWLTFSLRLPPEDYRLWLLLGEVSSKVQHLTGVPLRP
                     DVAADLHRVYLAKGVLATTAIEGNTLSETQVRRLVDGKLDLPKSQQYLQQEVQNILDV
                     CNREARELTRVEGGDRKLCRTLIEQYNRDVLKGLELSEGTVPGKLRPHSVLVGNVYRG
                     APAEDCEFLLEKLCTWLNSDDFSPAEEEFRIPFALIKAIVAHVYLAWIHPFGDGNGRT
                     ARLLEFHILFANGIPLPAAHLLSDHYNLTRVGYYRELARASASGGDLLPFIRYAVRGF
                     LDGIREQIGRIREQQMRVAWENYVYDRFRDAKNSPAQKRRRDLVFALTDRSGWVDVTD
                     IPLLTPKLAREYATAGERMVQRDLNALAKMELISRRHGKARANQHVIEAFLPRRVETS
                     K"
     gene            complement(4838..>5068)
                     /locus_tag="PK576_09655"
     CDS             complement(4838..>5068)
                     /locus_tag="PK576_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61894.1"
                     /translation="ATVRHKDPVLVAFTLPSDRTHGSPVGVDETQKLHEIRPGASMWV
                     HQPRFYEKSLLVAKVAPKNPERVVKLRHQKFF"
BASE COUNT          966 a         1532 c         1638 g          932 t
ORIGIN      
        1 tgccatgaaa ccaatgcggg tgccgccttg cgcgaaacaa ccgagcagcg cttttcctct
       61 catggttgcg agccactgcg tggccgcaac aagagaggtt gacccgcgac ccgcgacttg
      121 cggccagccc tgccccccaa aaccttcgct cccttggtgc cttcgcatga aaaaagcgtt
      181 cacctcagcc cggccagcgc caattcgtaa tccccagcgc gatttgatac agcccgaaac
      241 cggccagtgc gaaccccgag acgccggaca gaagccggtt caaccgctcg tcccttctcc
      301 cggcgcatgc gcacagcagg atgatccccg ccatactcgc caccatcgcg ccgtaaaacc
      361 ccgccagaaa accgatcccg ttgacaggcg tttcgcgcca ccctgacaac agcagcggcc
      421 cgcctactaa cccccaatac acataggggg atgggtttag caggttcagg agcacggcac
      481 ggaacacgcc tttccgatca gaacggtctg acggcgcaac cgtctcaggg tgtcgccaga
      541 aacgcaacgc ccccgccgca agatagagga tgaagaaacc gccagcgaac cgcaacagtt
      601 gtatccacca cggcgggacg cggctcagca cgatcagtac cagcacgatg atcgggccat
      661 cgctcagcag cggtgccagc acggcaggca acgcccgcct ccagccccga ctcaacgcca
      721 aagaaaccag ataggccaga aacgggccgg gctgcaccgc agccgcaaaa ccgtaaaccg
      781 cgccttgtac aaggtataga gacataagct tccttcttcc ttggtaagtg tatgactgtt
      841 atgacgctga aacaagcggg tatttgtctg cacaaaggac agaacaccgt gaggggggct
      901 gcggtcggaa agcggctacg cgcccaatga ttcttgtgcg gcggttcctt gtcagtgtcc
      961 ggcgcggtgt ggtataagga aaggcctttc tgctgtgtgc ggagcggaac ggaagagcgg
     1021 tatggctcga tgggggagtg gggcgcggca gaggacggaa gggggaggaa cgggatgcgg
     1081 atcggaatcg tgtggggaat ggtgatcgga tgggggacgg ttctcgcggc ggagccttgt
     1141 ctggaatacg tatatcccgc aggcgtgtgt gcggattcgg cgtgcgaggt cgaaatcggc
     1201 gggacgcatc tggggaatgt gacgcaagcg gtgatcagcg gtgaaggggt caaggcgttt
     1261 tttctgggtc cggttcggac ggtgacccgg aaccaaaagg ggcgcgaggt gcccgtcgtc
     1321 ctgccggacc ggtatcggtt ccgcgtggtc gccgatcccg aggccgcgcc gggtatccgc
     1381 gcgttgcgtg tggccacggc gcatcacctc agcgagcccg tgcggtttga ggtgggcacg
     1441 atgtccgagt cgagcgaacc agcgaccctt cgcaccgaaa cgggcgaggt cgtgctgtcc
     1501 aatctgccgg cctgtctgaa cggccgatgc cacggccggg gtgcggacgt gtaccgcttt
     1561 cgcgctgccc cagggagcgc ggtggtcgcg tttgtggagt cgcggaccgt gtcggccaac
     1621 ggttttgtgc ccgccctcac attcacggac gccgcgggaa aaccgtgcgc aaacatgaag
     1681 gcgtatgacg gggaggatgc gccggtgctc gtgctcgaag tgcgggagga gggcgagtac
     1741 agattgctgg tggcggcggg atcggatgcg gaaggcgggg acgggtgcgt gtaccggatc
     1801 aaactcggcg cgctgccgct ggtcacgggg ttgacgcctg acagggcacg ggagggcgag
     1861 agtctgaatg tgcgtttggc cggacataat ctcgcgcgga gccgcctgcg tcttttcaca
     1921 ggcggcaagg acagcgcgcg gtgcctggcg acgctcaccg agggcgcgtt ggccctgcct
     1981 tcgttgcgtt tcgatctggc caacgaagcg caggctccgg gattcgaggc gaccctgact
     2041 ccggcatcgc tgaacattcc gcctgacggc agcgcgctgg tacgggtgga ggtgcggcgt
     2101 cggaacggat tcgagggtgc ggtgcgcgtg gaactcgatt ttccgccgct cagtatcgcg
     2161 agccaaggcg gactgattcc cgccggggag agtttctgcc tgatgaccgt ttccaccgac
     2221 ggcgcccggt atccgcgcac ggtcttcagt ctgcccctca ccgccacggc ggagatcaac
     2281 gggcagccgg tcaagcggcc tgtcgtgccg gtgcggcaac gtgaggggaa tacggccgcg
     2341 cggccgcaat cttttgacga gtcggcagcc agggtcaatc ctaccctgta tgcgctgcac
     2401 ctgaacgtcg cgccccgcaa accgctttcc ctctcgacgg ggaagccgat ggaactgatg
     2461 atgatcagct ccacattggc ggcgcgcctg gggaacggat gcaagaccgt ggtggtctgg
     2521 cctgagcggg ggatcacggt tcaggacgtg cagcggacga accgtcagga acgcgcgcgg
     2581 gtgatgcttc gcacagatcc gtccgtgctg caggctggcg cctctggata tctcatcctc
     2641 ggttgtgtgg aaaaaaaggc gccgaaaggc cccgtgatgg cggtgacgca gtctgttccc
     2701 ttcaccgtga agtagggggg cgctggcaga cggggcagaa atgcgtgcca cgctgtccca
     2761 caacgcttcg cgcgatcgga cggccgcagc gcgggcacgg caggccgtca cgccggaaga
     2821 cccgcagatc gtcggcgttg cgtccgcggc gtcccgccac actgtaaaaa ttcgtctcgc
     2881 ccccgccgag cgtggtgccg cccttcgtga tggcgcgccg caggatcgtg cggatggccc
     2941 ggcacaaccg ctggatctcg gtttccgtca gcgagtcggc gcggcgttcc ggatggatcc
     3001 cggcgtgcca cagggattcg tccgcgtaga tattgccgag cccggccaca acggtctggt
     3061 tcaggagcaa cggcttgatt ttgccccgtt tgccggcaag gcgcgcgcgc agcacggctg
     3121 tcgtgaaggc cggctccagc ggttccggtc cgagcgttgc aaaggggtcc gtgtctgcac
     3181 cgcacaaccg gaggcgccca aacttgcggg tgtcattgaa tacgagtcgg cgggagtctt
     3241 ccagaagcag aagcgcgtgg tcatggcggc cgggccgttc ctccgcacca aggaaacgca
     3301 gtttcccggt catgcgcagg tggatcagca ggcggtcgcc cgtgtcgagc gtgaatacga
     3361 tgtacttggc gcggcgtgtc acgctggtga cggtccggcc ccgcagtgcg cgggcgagcg
     3421 acgcgggcga ccggccggcc gcgatccgcg gccagcggaa ccgcacttcg cggatcacgg
     3481 caccgaccat tccttgggca cggagttcgc ggacaacggt ttcgacttca ggaagttctg
     3541 gcataggcgc tcccgtattg gggggggtta tcccggtacg tgaggaatcg attattttga
     3601 cgtttccacg cgacgcggca gaaaggcttc gatgacgtgt tgattggctc tggcttttcc
     3661 gtgacgccgg gaaatcagct ccattttcgc gagcgcgtta agatcccgct gcaccatccg
     3721 ctcgcccgct gtagcgtatt cgcgcgcaag cttcggtgtg agcaagggaa tgtccgtcac
     3781 gtcaacccag ccgcttcgat cggtgagggc gaagacgaga tcccggcgac gcttctgcgc
     3841 gggggagttc ttcgcatccc tgaaacggtc atacacatag ttttcccacg ccacccgcat
     3901 ctgctgttcg cggatgcgcc cgatttgttc gcggatgcca tcaaggaagc cccgcaccgc
     3961 atagcggata aagggcagga gatctccgcc ggacgcgctt gcccttgcca gctctcggta
     4021 atagccgacg cgcgtcaagt tgtaatggtc gctcagcaga tgggcggcgg gaagcgggat
     4081 tccgttggcg aagaggatat gaaattcgag caagcgcgcc gtccgcccat tgccgtctcc
     4141 gaaggggtgg atccatgcca gatagacgtg cgccacaatg gccttgatga gtgcgaacgg
     4201 aatacggaac tcctcttctg ctggcgagaa atcatcactg ttgagccatg tgcagagctt
     4261 ttccaacagg aactcgcaat cctccgcagg cgcgccgcgg tagacattgc cgaccaacac
     4321 cgagtgcggc cgcagttttc ccggcacggt tccttccgaa agctccagcc ctttgagcac
     4381 atcgcggttg tactgctcaa tcaacgtacg gcagagtttc cggtccccac cctccaccct
     4441 tgtcagttcc cgtgcctcac gattacaaac atcaaggatg ttctgcacct cctgctggag
     4501 gtactgctgg gatttcggca aatccagttt gccgtcaacc aatcgccgca cctgtgtttc
     4561 gctcagggtg ttgccctcga tagcagtggt ggccagcaca cccttagcca gatagacacg
     4621 gtgcagatca gccgcaacgt ccggtcgcaa cggcaccccg gttaagtgct gaaccttaga
     4681 cgagacctcc cccagcaaga gccagagacg atagtcttca ggcgggaggc ggagtgaaaa
     4741 cgtcagccac gggtgggttg tttcatactt catggtggtc atcataacca attgccctgc
     4801 aggccgtcaa cgctactttg ggcttgaatt gaccttttca gaaaaacttt tggtgtcgca
     4861 atttaacgac cctttccggg ttctttggcg ccaccttcgc aaccagcaag gacttttcgt
     4921 aaaaacgggg ttgatgcacc cacatacttg cccccggcct gatttcatga agcttctgtg
     4981 tttcatcgac cccgactgga ctgccatgcg tgcggtcact tggcaaagta aatgcgacca
     5041 aaactgggtc tttatgacgc acggtcgc
//
LOCUS       DAOSLE010000176         4966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_15122_length_4966_cov_8.578294, whole genome shotgun sequence.
ACCESSION   DAOSLE010000176 DAOSLE010000000
VERSION     DAOSLE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15122_length_4966_cov_8.578294"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PK576_09660"
     CDS             <1..1165
                     /locus_tag="PK576_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOE61895.1"
                     /translation="AFASCTNLPAVTIPNGVRNIGRNAFGLCSSLTTVTIPNTVTNIG
                     EYAFTLCSSLTNTPLPEGVAAIGRCAFARCLSLSSVHIPASVAFIDEWAFVSCYALTS
                     ITVAGNNQHYASSDGVLFNKGLTTLVMCPSGYPGAYAIPDSVNTIADSAFTSCTSLTS
                     VAIHNGVTSIGASVFEHCDSLTSIGVAGGNPNYSSKDGVLFNKNRTTLIQYPNGLHGA
                     YAIPAGVTAIGDDAFRLSIFLTAVTIPASVTAIGDGAFSECPLLTSVLFKGNAPTMVG
                     VDIFYSTPDATVYYLPGATGWGDTFCGRPTALWNAAFNTTAPPQTDASGAFAFSLTGN
                     EGIPVCIEACDDLATQNWIILTNTTMSSDGTFDFTDPDAATLPTRFYRFNFPQ"
     gene            1362..2642
                     /locus_tag="PK576_09665"
     CDS             1362..2642
                     /locus_tag="PK576_09665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE61896.1"
                     /translation="MSETRNHAEHTAPASYKWLLVALLWVVFFLNQGNRQIYSTVLPQ
                     IRAATAQGGLGLSDVQAGLVATVFIAVYGVCVPLAGFLGDILRRKKQILFSLILFSAG
                     TLLTGFSGGMPGMILFYGILFGGGEALYYPPATSLLGQFHENSRATAFSIHQTALYLG
                     IMFSGYFSGYLARTMAGGWRAPFVVFGLAGIVWSGVVLFLMRDTPSPTRDAAAARVPL
                     RETLAHILSKRSAIALALAFGCMVYVDVGFKTWTPTFLIETFGFTPEKAGFSAVFYHF
                     VCAFVGVMIGGKLTDRWAAHRRTVRFEANAVGLLLGAPFILLMARSGSEWACFAALGL
                     FGLFRGVYDSNLFASLFDVIRPQYRASATGLMLSFAFLTGSLSPTVLGFFKARFGLSF
                     GLASLAFAYVLGGVIILTARLLFFGRDYEGRPAA"
     gene            2695..4050
                     /locus_tag="PK576_09670"
     CDS             2695..4050
                     /locus_tag="PK576_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HOE61897.1"
                     /translation="MRMRKQGRVLIAVALCVAGAEAELPAAYRALWEAPELTRRIEEG
                     IERNRKGDLALRVTDAAGRPVAGAAVTVRQTAHAFLFGCNAFVLGQLETPERNRLYEE
                     RFARICNFATVPIYWKGTEPRQGVLRYAEGGENIWRRPPVDRFIPFGRKYGLTLKAHP
                     MLWHEHNPDWLPRDPEALKALYGKRFRELAGRYAKEITFWEVTNESAGCPKTYPLYSE
                     DRAYVAWALREAAPLFGPENRLMINDFTRFNALPPARNTYHQQLRGLIDQGLRLDAIG
                     LQFHIWFEPRLMERHLAGERYRPADLLDTYEAFAAFGRPVYITEITVPTPEGPDGEEV
                     QAAVIRNLYRLWFSAPAMAGVTYWNLGDSMAHEKENTARAGLVDSALNPKPSYRALDE
                     LVNRIWRSRAEGTTDAEGRLRFRGFYGTYEATVTQGGKTRRIPARWSKGGGPEQRVVM
                     D"
     gene            4175..4963
                     /locus_tag="PK576_09675"
     CDS             4175..4963
                     /locus_tag="PK576_09675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE61898.1"
                     /translation="MNPNKQTMLKQAFSLEGRTALVTGSARGIGRAIARALAECGATV
                     AVHGVRASDALDQSLADVRAFAPQSAAVTGDLAESGMGARLVAAVGAAVGAPDIVVAN
                     ASVQVRRPWLEVPESEALLQMQVNFHATYQLFQAAYPAMAARHWGRLISVGSVQELKP
                     HPEMPVYAASKAALENLVRNLARQAAPDGVTVNNLCPGVFATDRNAEALGNPVYAEKV
                     RAAIPLRDFAQPEDAAGAALLLCSDAGRYITGVTLRVDGGMGVG"
BASE COUNT          807 a         1643 c         1636 g          880 t
ORIGIN      
        1 cgccttcgcc tcctgcacta accttccagc cgtcaccatc cccaacggcg tcaggaacat
       61 tggcagaaac gccttcgggc tttgcagctc cctgaccacc gtcacgattc ccaacacggt
      121 caccaacatc ggcgaatacg ccttcacctt atgctcctcc ctcacgaaca cccccctccc
      181 cgagggcgtc gccgccatcg gcaggtgcgc gttcgccaga tgcctgtcgc tttcatccgt
      241 ccatatcccc gctagcgtcg ccttcatcga cgaatgggcc ttcgtctcct gctacgcgct
      301 caccagcatc actgtcgcgg ggaacaacca gcactacgcc agcagcgacg gtgtgctctt
      361 caacaagggc ctcaccacgc tggtgatgtg tcccagcggc tatcccggcg cctacgccat
      421 cccagacagt gtcaacacca tcgccgatag cgccttcacc tcctgcacct ccctcacttc
      481 tgttgccatc cacaatggcg taacgtccat aggcgcctcg gttttcgagc attgcgattc
      541 cctgacgagc attggcgtcg cggggggaaa cccgaactac tccagcaagg acggtgtgct
      601 gttcaacaag aaccgcacca cgctgattca atatcccaac ggtctccatg gcgcttacgc
      661 catccccgct ggcgtcaccg ccatcggtga cgatgccttc aggctcagca tcttcctcac
      721 ggccgtcacc atccccgcca gcgtcaccgc catcggtgat ggcgccttct ccgaatgccc
      781 cttgctcaca tccgtccttt tcaaaggcaa cgccccgaca atggtcggtg ttgacatctt
      841 ctacagtaca cccgacgcca cggtctacta cctccccggc gccacgggat ggggcgacac
      901 cttctgcgga cgtcccaccg ccctctggaa tgccgccttc aacacgaccg ccccgccgca
      961 aaccgacgcc tcgggcgcgt ttgccttcag cctcaccggt aacgagggca tccccgtctg
     1021 catcgaggcc tgcgacgacc tcgccaccca gaactggatc atcctcacca acaccacgat
     1081 gtccagcgac ggcacctttg atttcaccga ccccgacgct gccaccctcc ccacgcgctt
     1141 ctaccgcttc aacttcccgc agtagaagga ggggggcgac aggtgctcca gtctgcggca
     1201 tcgcttgatc cgacgaatcc gcctgatctg actgatccgt cattgcgggc ggggacgccc
     1261 gcgctcccga cacctgacgc cggttttttg cgcaaacgca tctggcttgg cggcgcgtga
     1321 tgcggtacca tgctctcgga tcactaccaa ggggggaaga gatgtcggaa acccggaatc
     1381 atgcggaaca cactgcgccc gcgtcttaca aatggctgct ggtggccctg ctctgggtcg
     1441 tctttttcct gaatcagggg aaccggcaga tttacagcac cgtgttgccg cagatccggg
     1501 ccgcgaccgc gcagggcggg ttggggctga gcgatgtgca ggccggattg gtcgccacgg
     1561 tgttcatcgc ggtctacggc gtctgcgtgc cgctcgccgg atttctgggc gacatcctgc
     1621 gccgcaagaa acagatcctg ttcagtctga tcctgttcag cgcggggacg ctgctcaccg
     1681 ggttcagcgg cgggatgccg ggaatgatcc tcttttacgg catcctcttt ggcgggggtg
     1741 aggcgctcta ctatccgccc gccacgtcgc tcttggggca gttccatgag aacagccggg
     1801 ccacggcctt ttccatccat cagacagccc tgtacctcgg catcatgttc agcggctatt
     1861 tttcggggta tctggcccgg acaatggcag gaggctggcg cgcgcctttt gtggtgttcg
     1921 ggctggcggg gatcgtctgg agcggggtgg tgctgttcct catgcgggac acgccgtcgc
     1981 caacgcgcga tgccgccgcg gcgcgtgtgc cgctgcgcga gaccctggcg catattctga
     2041 gcaagcggtc cgccatcgcg ctggccttgg ccttcggctg catggtctat gtggacgtgg
     2101 ggttcaagac ctggacgccc acgttcctga tcgagacctt cgggttcacg ccggagaagg
     2161 ccgggttctc ggcggttttt taccattttg tctgcgcctt cgtgggcgtg atgatcggcg
     2221 ggaaactgac ggaccgctgg gccgcgcacc gcaggacggt ccggttcgag gcgaacgccg
     2281 tggggctgct cttgggcgcg ccgttcatcc tcctgatggc ccggagcgga tccgaatggg
     2341 cgtgtttcgc cgcgctcggc cttttcggcc tgttccgcgg ggtctacgac tccaacctct
     2401 tcgcctcgct gtttgatgtg atccggcccc agtaccgcgc ctccgcgacc gggctgatgc
     2461 tctccttcgc gtttttgacg ggttcgctct cgccgaccgt gctgggattt ttcaaggcgc
     2521 gcttcgggct gtcgttcggg ctcgcctcgc tcgcgttcgc gtacgtgctg ggcggggtga
     2581 tcatcctgac cgcccgcctg ctgtttttcg gacgcgatta tgagggcagg cccgctgcct
     2641 gagcgcggat cgcacaaaaa ggatatggca tcaagaacaa gaatgaggac gaggatgaga
     2701 atgagaaaac aaggacgggt gctgattgcg gtcgcgctgt gcgtggccgg tgcggaagcg
     2761 gaactgcccg cagcctatcg cgcgctctgg gaggcgccgg agttgacgcg gcggatcgag
     2821 gaggggatcg agcgcaaccg caagggggat ctcgccctgc gcgtgaccga cgctgcgggg
     2881 cggccggtcg cgggtgccgc ggtgacggtg cggcagaccg cgcacgcgtt cctgttcggg
     2941 tgcaacgcgt ttgtgctggg tcagctcgag acgcccgagc gcaaccggct ctatgaggag
     3001 cggttcgccc ggatctgcaa cttcgccacg gtccccattt actggaaggg gaccgagccg
     3061 cggcagggcg tgctgcgcta cgccgagggg ggcgagaaca tctggcgtcg gccgccggtc
     3121 gaccgcttca tcccgttcgg caggaaatac ggtctgacgc tcaaggcgca cccgatgctg
     3181 tggcacgagc acaaccccga ttggctgccc cgcgacccgg aggcgctgaa ggccctctac
     3241 gggaagcggt tccgcgaact cgccggacgc tacgcgaagg agatcacgtt ctgggaggtg
     3301 accaacgaat cggccggctg ccccaagacc tacccgctct actcagagga tcgggcgtat
     3361 gtggcctggg ccctgcgcga ggccgcgccg ctcttcgggc cggagaaccg gctgatgatc
     3421 aacgatttca cccgcttcaa cgccctgccg cccgcgcgca acacctatca tcaacagctc
     3481 cgcgggttga tcgatcaggg gctgcgcctc gacgcgatcg ggctgcagtt ccacatctgg
     3541 ttcgagcccc gcctgatgga gcgccatctg gctggcgaac gctaccggcc cgccgacctg
     3601 ctcgacacgt acgaggcgtt tgcggcgttc gggcgtccgg tgtacatcac cgagatcacg
     3661 gtgccgacgc cggagggtcc ggacggcgag gaggtgcagg ccgcggtgat acgcaacctc
     3721 taccgcctct ggttcagcgc gccggccatg gccggggtca cctattggaa tctgggcgac
     3781 agcatggcgc acgaaaagga gaatacggcg cgggccggtc tggtggacag cgcgttgaat
     3841 cccaaaccct cctaccgcgc gctggacgag ctggtcaacc gcatctggcg cagccgtgcc
     3901 gagggtacga cggatgcgga ggggcgcctg cggttccgag ggttttacgg gacgtacgaa
     3961 gcgacggtca cgcagggcgg caaaacgcgc cggatccccg cccgctggtc gaaaggcggg
     4021 gggccggaac agcgcgtggt gatggattga gggagggagg acaggagacg ggagacggga
     4081 gacgggagtc gggagcgcgg gcgtccccgc ccgctggccg aaaggcggcg ggccgctgaa
     4141 cgtcgacacg aaaaacgctg cacaggacaa aacaatgaat ccgaacaagc agacaatgtt
     4201 gaagcaggct ttttcattgg agggccgcac cgccctggtg accggttccg cgcgcggcat
     4261 cggccgggcg atcgcgcggg cgctggccga atgcggggcc acggtcgcgg tgcatggcgt
     4321 gcgggcgagc gacgcgctcg accagtcgct ggccgacgtg cgggcgttcg cgccgcagag
     4381 cgcggcggtg accggcgatc tggccgagtc cggcatgggg gcgcggctcg tggcagcggt
     4441 cggcgcggcg gtcggcgcgc cggacatcgt ggtggcaaac gcctcggtgc aggtccgccg
     4501 gccgtggctc gaggttccgg agtcggaggc gctgctgcag atgcaggtga acttccacgc
     4561 cacgtaccag cttttccagg ccgcctatcc cgcgatggcg gcgcggcact ggggccggct
     4621 gatctcggtg ggcagcgtgc aggagctgaa gccgcacccc gagatgccgg tgtacgcggc
     4681 gagcaaggcg gcgctggaga atctggtgcg gaatctcgcc cggcaggcgg ctccggacgg
     4741 ggtgacggtc aataacctct gccccggggt cttcgccacc gaccgcaacg ccgaggcgct
     4801 cggcaatccc gtctacgccg agaaggtgcg cgccgccatt ccgctgcgcg acttcgcgca
     4861 gccggaagac gcggcgggcg cggcgctcct gctctgttcc gacgcggggc ggtatatcac
     4921 gggcgtcacg ctgcgcgtgg acggcggcat gggggtgggg tagagg
//
LOCUS       DAOSLE010000177         4841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_15603_length_4841_cov_9.142917, whole genome shotgun sequence.
ACCESSION   DAOSLE010000177 DAOSLE010000000
VERSION     DAOSLE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4841
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_4841_cov_9.142917"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..834)
                     /locus_tag="PK576_09680"
     CDS             complement(82..834)
                     /locus_tag="PK576_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61899.1"
                     /translation="MKFLKNIPPPETFRDFSEPRAFSIWMRWACGFAAGVCLIGLAVC
                     VALDGCGKRKDGHDAETAEAEKPSVPPPDLHNPVREAPATFPPDSNLPRGKEIYASGP
                     IDLAQFDPARDLVRFEDERVWFESDHDGPEDDEDDHQIHRAVEVPLRRLVNLLERRGG
                     KLKVQEAYRAASSKNRIHLPSSLHREGRAVDLTCEGLPLSELAKLAWQAGFDFVLYEV
                     PKRSGPHLHCSVRRAPDAPLPPRPGGQSPQAP"
     gene            1106..3175
                     /locus_tag="PK576_09685"
     CDS             1106..3175
                     /locus_tag="PK576_09685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE61900.1"
                     /translation="MSDSPQTMKMLPGDEIRQIMWRYADRYDIQMAVMGARSVARGLV
                     ARLVADGERATHEWTEAKNSLYQAFDESGITAAGLDMEYGGIIEGPRNLALSLIAFEL
                     SWVDGGASTTSLINNLGLGPIVEKGTPEQREKYMRVCAPAQPGEDRKPWRAAFALTEP
                     LPYVGVDTGVVSGRVTVAKWEEGQEPILHVEKRGRFLTNMAVANVLTAAVDTGDPRIK
                     SSCMVILEDTDPGVFDRGAPTLKMVHQLSNTHDPIFSLDVPASRIVGGYTVKDGVIVP
                     NLTHSEIIEAVFSRTRVGVGLMTAAKLLSAVEPVIRYHRGRYRGAAGIEAGAPRYDQG
                     IQMKEDAVQRLADVWATGEAAASLGFETARAFDALTPVENQALADFAAQGVSGRALMR
                     ALRKPQADAIELLDQLGKPEAERDEARVAALQADPLVQYVWLSALCNVLCPATKLWDT
                     GHGANMLREAVSLMGGYGITEDCPGFLFYKWTDAQLEATYEGPEVVQRRQISVTMNNE
                     VFLAQVAQWIAELRRQNAAAPGNGLDTLAEGFALWRWTLAFIQSAKDAEGRALSQSQR
                     HGVLFPMADAISWLLAARSLVADIQELAAKGPGHPVVGPEIAGYVNTFTDLAHMQIAR
                     AVGETARICAELVYGYGAATAEQSSEFQTLRAKADAAMAGARLAKDRAGRALAQVMIP
                     EALDYPQ"
     gene            3536..4573
                     /locus_tag="PK576_09690"
     CDS             3536..4573
                     /locus_tag="PK576_09690"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOE61901.1"
                     /translation="MKNKCLWGLVAGVLSGIVPHAAAQVVSSVAMPVIQLTGTNEVEL
                     GKITCEELKLITFVFRNPGSVPVEIKQITSTCPCIRGYPEQAVIPPNGELPITAELNP
                     LKVKSVFKRGLWIHTTDPRQVRIPLIVSGEVVPFFEGFPQEPVQFRRGAPGEAWTNVF
                     PLTATLTNLSLGTPVITKSKDMRVTASVAANQAQAGSFDLTLVAVPLVAGRSSATVEI
                     PIAGRPDLPNLDLLLYARVGTELYVTPSKFLMLPSDQPLTRGYLIRTGETEADPKQVA
                     FSPEREGVTYDVRPSKRKKAHLSLRVTVTPDALARLMREKEPKLRISYPNYKEAEITF
                     IPHMPPGPEQK"
BASE COUNT          887 a         1692 c         1434 g          828 t
ORIGIN      
        1 gaaaccatga aaccctgaaa ccaaagagcg ttgctttcaa cgctcttttt aaggcgtccg
       61 cgggcgggga cgcccgcgcc gctaaggcgc ttgcggactc tgtccgcccg gccgcggcgg
      121 cagcggcgcg tccggggcgc gccggaccga gcagtgcaga tgcggcccgg agcgtttcgg
      181 cacctcgtac agaacaaagt cgaaccccgc ctgccaggcc agcttcgcca actcgctgag
      241 cgggagcccc tcgcaggtca ggtccacggc gcggccctcg cggtgcaggg aagaggggag
      301 gtggatgcgg ttcttgctgc ttgccgcacg gtacgcctcc tgcaccttga gcttcccgcc
      361 gcggcgctcc agcagattga ccagccgccg gagcggcacc tccaccgcgc ggtggatctg
      421 gtggtcgtcc tcatcgtcct ccggcccgtc gtgatcgctc tcgaaccata cacgctcatc
      481 ctcaaaccgg accagatcgc gggcaggatc gaactgcgcc agatcgatcg gccccgatgc
      541 gtaaatctcc ttgccgcgcg gcagattgga gtccggcgga aaagtggcgg gcgcctcccg
      601 caccgggttg tgcaggtccg gcggcggcac cgacggcttc tctgcctccg ccgtctcggc
      661 gtcgtgcccg tccttccgct ttccgcaccc atcgagggcg acacaaaccg ccagcccgat
      721 caggcacacc cctgccgcaa acccgcatgc ccagcgcatc cagatactga acgcccgcgg
      781 ttcactgaaa tcccggaatg tctcgggcgg ggggatgttt ttcaaaaact tcatgcgcat
      841 cctcttcgga aacaaaccct gaactatact gaaggcgact ccataatgaa accaaaaata
      901 agtgccgcct cccttttccg cctccctttt ccgtctcctg tctcccgtct cttgtctccc
      961 gtctcttgtc tcccgtctcg tgtctcccgt ctcccgtctc ccgccacccg tctcccgtct
     1021 cccatgccgc caacctttcc ccttgcgtct tcgacaaaaa accataaaat gtcatcctca
     1081 aattcgatca agaaaggtgc tgacgatgag cgattctccg cagaccatga aaatgttgcc
     1141 cggcgacgag atccggcaga tcatgtggcg ctatgccgac cggtacgaca ttcaaatggc
     1201 cgtgatgggt gcccgttccg tggcccgcgg cctcgtggcc cgcttggtgg ctgacggcga
     1261 acgcgcgacg cacgagtgga ccgaggcgaa aaacagtctc tatcaggcgt tcgacgagtc
     1321 gggcatcact gcggcgggac tcgacatgga atacggcggc atcatcgaag ggccgcgcaa
     1381 tctggcgctg agcctgatcg cctttgaact ctcctgggtc gacggcgggg cctccaccac
     1441 cagcctgatc aacaacctcg gcctcgggcc gatcgttgag aagggcacgc ccgagcaacg
     1501 cgagaagtac atgcgcgtct gcgccccggc ccagcccggc gaggaccgca aaccgtggcg
     1561 cgccgccttt gccctcaccg agccgctgcc ctatgtgggc gtggacaccg gcgtggtcag
     1621 cggccgcgtg accgtggcca aatgggaaga ggggcaggag ccgatcctgc atgtcgagaa
     1681 gcgcggccgc ttcctgacca acatggctgt cgccaacgtg cttaccgccg cagtcgatac
     1741 cggcgacccg cgtatcaaat cctcctgcat ggtgattctc gaagacaccg atccgggcgt
     1801 cttcgaccgc ggcgcgccga ccctcaaaat ggtccaccag ctttccaaca ctcacgaccc
     1861 gatcttcagc cttgatgtcc ccgccagccg tatcgtcggc ggctacacgg tcaaggacgg
     1921 cgtgatcgtg cccaacctga cccacagcga gatcatcgag gcggtcttct cgcggacgcg
     1981 cgttggggtc ggcctgatga ccgccgccaa gctgctctcc gctgtcgagc cggtcatccg
     2041 ctaccatcgc gggcgttacc gcggcgcggc cggcatcgag gccggcgcgc cccgctacga
     2101 tcagggcatc cagatgaagg aggacgccgt tcagcgcctc gccgatgtct gggccaccgg
     2161 cgaagccgcg gcctcgctcg ggttcgagac cgcgcgcgcc tttgacgccc tcacgcctgt
     2221 tgaaaaccag gcgctcgccg atttcgccgc ccaaggcgtc tccggccgcg ccctgatgcg
     2281 ggcgttgcgc aaaccgcagg ccgacgccat cgagctgctg gaccagcttg gcaagcccga
     2341 ggccgagcgc gacgaagccc gcgtcgcggc gctgcaggcc gatccgctcg tgcagtacgt
     2401 ctggctcagc gcgctctgca acgtgctctg cccggccacc aagctgtggg acaccggaca
     2461 cggggccaac atgctgcgcg aggcggtcag cctgatgggc ggctacggca tcaccgaaga
     2521 ctgccccggc ttccttttct acaaatggac cgacgcccag ctcgaggcca cctacgaagg
     2581 cccggaagtg gtgcagcgcc gccagatcag cgtcaccatg aacaacgagg tcttcctggc
     2641 ccaggtcgcc caatggatcg cggagctgcg ccgacagaac gccgccgcgc ccggcaacgg
     2701 cctcgacacg ctggccgaag gcttcgcgct ctggcgctgg accctcgcct tcatccagtc
     2761 cgccaaggat gccgaaggcc gcgcgctctc gcagagtcag cgccacggcg tgctcttccc
     2821 gatggccgac gcgatctcgt ggctgctggc cgcgcgttca ctcgtcgccg acattcagga
     2881 gctcgccgcc aaaggccccg ggcacccggt ggtcgggcct gagatcgcgg ggtacgtcaa
     2941 caccttcacc gatctggcgc acatgcagat cgcgcgcgcc gtcggcgaga cggcccgcat
     3001 ctgcgccgag ctggtttacg gctacggcgc ggccacggcg gagcagtcgt ccgagttcca
     3061 gacgctgcgc gccaaggcgg atgccgcgat ggccggcgcg cgcctcgcca aagaccgcgc
     3121 tggccgcgcg ctcgcgcagg tcatgatccc cgaggccctc gactacccgc agtgatcctt
     3181 tcagccggga gcgcgggcgt ccccgcccgc aatggcggat cagtccgatc ggactgatcc
     3241 gccggatccg ccgccttgcc aacaacccaa acgccgcggg tcgaatgtcg cgggtcgcgg
     3301 gtcgacgata ccgatagcga taccgatagc gataccgacc ccgaccccga tttggatgaa
     3361 accctgaaac cagaaaccat gaaaccatga aaccctgaaa ccaaagcggg cggggacgcc
     3421 cgcgctcccg actttcccgc gctcccgact ttctgcccgc ctccttttta attcctcctt
     3481 tcttttttcc gattgcattg atgcgcctgc cgggatgtga tatcgttttt cccttatgaa
     3541 aaacaagtgc ctttggggtt tggtggctgg cgtgttgtcg gggatcgttc cgcatgcggc
     3601 ggctcaggtg gtttccagcg tcgccatgcc ggtgatccag cttaccggga ccaacgaggt
     3661 cgagctgggc aagatcacct gtgaagagtt gaagctgatc acgttcgttt tccggaatcc
     3721 cggaagcgtg ccggtggaga tcaaacagat cacctcgacc tgcccctgta tccggggtta
     3781 tccggagcag gcggtcatcc ccccaaacgg tgaactcccc atcaccgctg aactgaaccc
     3841 cctcaaggtc aaaagcgtct tcaagcgggg cctgtggatc cacaccaccg atccgcggca
     3901 ggttcgcatc ccgctgatcg tcagcgggga ggtcgtgccg tttttcgaag gctttccgca
     3961 agagcccgtg cagttcaggc ggggcgcgcc cggcgaggcg tggacaaacg tgttccccct
     4021 cacggccacg ctcacaaacc tctccctcgg aacaccggtc atcacaaaaa gcaaggacat
     4081 gcgtgtcacc gcctcggtcg ccgcgaacca ggcccaggcg ggatcgttcg acctgacgct
     4141 cgtggccgtg ccgctggttg ccgggcgcag tagcgccaca gtggagattc cgatcgcggg
     4201 aaggcccgat ctcccgaatc tggatttgct gctgtatgcg cgcgtcggaa ccgaactgta
     4261 cgtcacgccc tcaaagtttc tcatgctccc ttcagaccag ccccttacgc gggggtatct
     4321 gatccgtacg ggcgagaccg aagcggatcc gaagcaggtg gccttctcgc cggaacgcga
     4381 gggcgtgact tatgatgtcc gccccagcaa acggaaaaag gcgcacctct cgctgcgcgt
     4441 caccgtcact ccggatgcgc tcgcccggct catgcgcgag aaggagccca agctgcgcat
     4501 cagctatccc aattataaag aggcggaaat cacgttcatc ccccacatgc cgccgggtcc
     4561 ggaacagaaa taagccgcgc ccgatagcga tgcttcccgc ccgcaatggc ggatcagtcc
     4621 gatcggactg atccgccgga tccgccgcct tgccaacaac ccaaacgccg caggtcgaat
     4681 gtcgcgggtc gcgggtcgcc gatcccgata gcgatagcga taccgatacc gataccgata
     4741 ccgaccccga tttggatgaa accctgaaac ccagaaaccc tgaaacccag aaaccctgaa
     4801 accaaagagc gttgctttca acgctctttt taaggcgtcc g
//
LOCUS       DAOSLE010000178         4827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_15652_length_4827_cov_5.362951, whole genome shotgun sequence.
ACCESSION   DAOSLE010000178 DAOSLE010000000
VERSION     DAOSLE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15652_length_4827_cov_5.362951"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1277
                     /locus_tag="PK576_09695"
     CDS             177..1277
                     /locus_tag="PK576_09695"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE61902.1"
                     /translation="MLRDCEAITDKGVTYAALVLFGRREALGRLLGQAEVIFEYRSSE
                     ASGPAQQREEFRQGFFSFHDRLWELINLRNNKQHYQDGFFVMDIPTFDERVVREAVLN
                     AVCHRDYQLGGSVFVRQFQHRLEVDSPGGFPPDITLENILDRQSPRNRRIADILSRCG
                     MVERSGQGMNLMFELSIKQAKALPDFTGTDRMHVRVTLHGLVQDPKLLTMMERIGMEK
                     LESFSTGDLLVVNHIHREQAIPDGLRQRIPRLLELGVIERVARGKFILGRQYYTATGK
                     KGTYTRKRGLDHETNKALLLKHIRENADVGSRLEELLQVLPNLSRSQVQWLVRQLRTE
                     NKVRLEGVTRSGRWFPTITVADCAKTDVEKQS"
     gene            1443..4760
                     /locus_tag="PK576_09700"
     CDS             1443..4760
                     /locus_tag="PK576_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOE61903.1"
                     /translation="MAKLPWKPWHEVVTLRDDLRSGELPMNLFAADLYEVMMQSGKRP
                     IYEQPEHFFALTFPTYNLRQLVRDVVLRLAGKNDKAVRQLALTYGGGKTHTLITLRHL
                     VTDPANLPNLPAVAEFIEAIGEQPPPCRVAALCFDKLDVEKGMEARAPDGSLRTLKHP
                     WSVLAYQLAGDAGLKLIHADGKAEERETAPAENLLSELLAIPVKDGLGVLILIDEVLM
                     FARGKVALDRAWLGKLQNFFQYLTQAASKSDRCCIVASLLASDTDMSDTFGRGVDAAL
                     QDVFQRQREVVVEPVVKEDVAEVLRRRFFTPESLKNRETFPQHVVAALKGIAAVDEQT
                     AKQGAEAEERFLRSFPFHPDLTEVLYSKWTQLSRFQRTRGVLRTFALALREAVQWDTN
                     PLVGPAVFLTAPDKEGLSEALRELVAVADTEVWEGKQQAWTGILDSEFSQARQIQTES
                     VGLKHREIEQAVVATFLHSQPIGQTAHTRDLTVLLSAARPDKIELEKGLVRWAQASYW
                     LDDQHTADSGGQLPDTWRLGNRPNLTQMHAVAAKNIQDNTVRARLIDDIGKTKALTAN
                     ASAAGVRTHTLPMQPKDIEDDGAFHYAVLGPNAASESGKPSAEAKRFLDETTGADRPR
                     VYRNAVLLVTPSKDGLELALARVRDYLAWEAVRDDVKKQQRDGDVDPARMQTLQINID
                     KAKGRIPDAIRQAYSIVVTVSDKNEAQAFRLTVTDEPHFNIIKSDERSRIKDTAITAE
                     ALLPDGPYNLWRKGETARRVRDLSGSFAQLPHLPKMLKASAILDTLVDGCEQGTFVLR
                     LTRPDGTWRSWWMSRPDENALNDPALELVLPEAAELSEIEPFLLAPKKLPGLWATDEI
                     TAKTVSEYFSGGTVVQIERDGYQEPMQIPKASQTVVETAIATAVVDGIVWLISGPASI
                     LSEPIPEGVLHADSKLCLPPAPIAAAEILPENLKDAWKDKIASGLSVATALSMKAGKT
                     LPWKTIKDAINSALQARFLKLASDSQTWPCDFPSAQFVKLTESSDPPDEAGKDDFWRE
                     DDKKLVAAATLEPSQIQDLGDVMPKLLELRSKSNTPLQFHIQIEMGDGMTKPAEETVQ
                     KANAILKSVNEGLEFHF"
BASE COUNT         1148 a         1344 c         1434 g          901 t
ORIGIN      
        1 cagaaaggcg aagacaagga acggtgaaat gatagcggct cggctgaaaa ccaacaagct
       61 ttgtgtcggt cgacggcgca cgtactgagg gtgctgaaaa atgaaatgtc caatgtcaaa
      121 ctacgaccct gataaccact ggccgtcccg gactagccaa tctcacggat gaacagttgc
      181 ttcgcgattg cgaggccatc accgacaaag gggtcaccta tgcggcactc gttctattcg
      241 gtcgccgtga agcactcggg cgtctgctag gacaggcgga agtgattttt gagtaccgat
      301 cgtcggaagc atccggcccc gcccaacaac gtgaggagtt ccgccaaggt ttcttttcat
      361 ttcatgaccg tttgtgggaa ctcataaatc tacgaaacaa caaacagcat taccaggacg
      421 gatttttcgt catggacata ccgaccttcg atgagcgcgt ggtacgcgaa gccgtgctaa
      481 acgcggtctg ccatcgcgac taccaattgg gaggctcagt gtttgtgcgg cagttccagc
      541 atcgtctgga agtggacagt cccggtggtt ttccgccgga cattacgttg gagaacatcc
      601 ttgatcgaca atcaccccgt aataggcgaa ttgccgacat actgtcacga tgcggaatgg
      661 tggagagatc cggacaggga atgaacctga tgttcgaatt aagtatcaaa caggcaaagg
      721 ccctgccgga cttcacggga accgaccgaa tgcacgtcag ggtcacgttg catggcttgg
      781 tgcaagaccc gaagttgcta accatgatgg agcggatcgg catggaaaaa cttgagagtt
      841 tcagcaccgg tgatcttctg gtcgtgaatc atatccaccg cgaacaagcc atacccgatg
      901 gtctgcggca gcggatacct cggctactcg aactgggcgt aattgagcga gtagcacgag
      961 ggaagttcat tcttggtcgg caatactaca ccgctactgg gaagaagggc acctacaccc
     1021 ggaaacgggg attagaccac gaaacaaaca aggcgctttt acttaagcac atccgagaga
     1081 atgcggatgt tgggagcagg ttggaagagc tgcttcaggt tttgccaaac ctttcacgaa
     1141 gccaagtcca atggctcgtt cggcagctca ggacagaaaa caaggtgcgg ctggaaggcg
     1201 tgacacgatc gggacgatgg tttcctacaa ttacggtggc agattgcgct aaaaccgacg
     1261 tggaaaagca atcatgacgc aatcccgcgc aaagcaatat ctcgtaacca ccttaattgc
     1321 aacaggttgc gcgtattgcg ttaagtgtca cacaactgta atagtaagca atcataagca
     1381 atcataagca atgattaggc tgtgataagc aacgataggc aatctgaaag aggagaacga
     1441 caatggcaaa actaccctgg aagccttggc atgaagtggt cacgctgcgg gacgatctgc
     1501 gttccggcga actaccgatg aacttgtttg ccgcagacct ctatgaggtc atgatgcaga
     1561 gcgggaagcg tccgatctac gagcagccgg agcatttctt cgcgctgacc ttccccacct
     1621 acaacctgcg tcaactggtg cgggatgtgg tgctgcggct ggcgggcaag aacgacaagg
     1681 ctgtccgcca gttggccttg acctacgggg gcggcaagac gcacactttg atcacgctgc
     1741 ggcacctggt cactgacccc gcaaacctgc cgaaccttcc cgccgtggcg gagttcattg
     1801 aggcgatcgg ggagcaaccg ccgccgtgcc gtgttgcggc gctttgcttc gacaagctcg
     1861 atgtcgaaaa ggggatggag gcgcgcgcgc cggacggcag cctccgcaca ctcaagcatc
     1921 cgtggagcgt gctcgcctac cagttggcgg gtgatgcggg ccttaagttg atccacgccg
     1981 acggcaaggc cgaagagcgc gaaacggcac ccgccgagaa ccttttgtcg gagttgctgg
     2041 cgataccggt caaggatgga cttggtgtgc tgatcctcat cgatgaggta ctgatgtttg
     2101 cccgtggaaa ggtcgcactc gacagggctt ggctgggcaa gttgcaaaac ttctttcagt
     2161 atctcacaca agcagcctcc aaaagcgacc gttgctgcat cgttgcctcg cttcttgcca
     2221 gcgacaccga tatgagcgac acattcggtc gcggcgtgga tgcggcgctt caggatgtgt
     2281 tccaacgtca acgcgaagtg gtggttgaac ccgtcgtgaa ggaagatgtg gccgaggtgt
     2341 tgcgccgccg cttcttcaca cccgaatcct tgaagaaccg cgagaccttc ccgcagcatg
     2401 tggtggcggc gctcaagggt atcgccgccg tggacgaaca gacggcgaaa cagggggcgg
     2461 aggccgaaga gcgtttcttg aggagcttcc cgttccatcc cgacctaact gaggtgcttt
     2521 actccaagtg gacgcagctt tcccgattcc agcgcacgcg cggcgtcctg cggactttcg
     2581 cgctggctct gcgcgaagcc gtgcagtggg acacgaaccc actggtaggg ccggcggtct
     2641 ttttgaccgc gccggacaaa gaggggctct ccgaagcatt gcgcgaactg gtggccgtgg
     2701 cggacacgga ggtatgggaa ggcaagcagc aggcgtggac cggtattttg gacagcgagt
     2761 tcagccaagc gcgccagatt cagaccgaat cggtaggact gaagcaccgg gaaatcgaac
     2821 aggcggtcgt ggcgacgttc ctgcattcgc aaccgatcgg ccaaaccgcg cacacacggg
     2881 atctgaccgt gctgttaagc gccgcgcgtc ctgacaagat cgaactggag aaggggctcg
     2941 tgcgttgggc gcaggcgagc tactggctgg acgaccagca cacggcggat tccggggggc
     3001 aacttccgga tacctggcga ttgggcaatc gcccgaacct aacgcagatg cacgcggtcg
     3061 cggccaagaa catccaggac aacaccgtgc gcgcgcgtct gatcgatgac atcggcaaga
     3121 cgaaggcact caccgccaac gcatcggcgg caggtgtccg cacccatacg ctgcccatgc
     3181 aacccaagga catcgaagac gacggcgcgt tccactatgc ggtgctggga ccgaacgccg
     3241 catcggaatc cggcaagccc agcgcggaag ctaagcggtt tttggatgag acgaccggag
     3301 cggatcgacc gcgtgtctac cgcaatgccg tcttgcttgt aacaccgtcg aaggacggtc
     3361 tggaactcgc gctggcccgc gtacgtgact atctcgcgtg ggaagccgtg cgggatgatg
     3421 tcaaaaaaca gcaaagggac ggggacgttg acccggcacg gatgcagacg ttgcaaatca
     3481 acatcgacaa ggccaaaggg cgtattccag atgccatcag gcaggcctat tccatcgtgg
     3541 tcaccgtctc cgacaagaac gaggcgcagg catttaggct cacggtgacg gatgaaccgc
     3601 acttcaacat catcaagtca gacgagcggt cacgtatcaa agacacagcc atcaccgccg
     3661 aggctctgtt gccggacggc ccctacaatc tctggcggaa gggcgagacc gcacgccggg
     3721 tgagggactt gtccggttcc tttgcgcaac tcccccacct gcctaagatg ctgaaggcca
     3781 gcgcgattct ggatacgttg gtggacgggt gtgaacaagg cacgttcgtg ctacgtctga
     3841 cgcggcctga cggcacatgg cgttcctggt ggatgtcccg cccggacgag aacgccctca
     3901 atgatccggc actggagttg gttctgccgg aggcggcaga gttgagcgaa atcgaaccgt
     3961 ttttacttgc tccaaagaag ctgccgggat tgtgggccac cgacgaaatt accgcaaaaa
     4021 ccgtgtccga gtatttcagc ggcgggacgg ttgtccagat tgaaagggac gggtatcagg
     4081 agcccatgca gattccaaaa gcgtcgcaaa cggtcgtcga gacggcaatc gccaccgccg
     4141 tcgtggatgg catcgtctgg ctgatctccg gtccggccag catcctcagc gaaccgattc
     4201 ctgaaggcgt gttgcatgcg gacagcaaac tctgcttacc gcccgcaccc atcgccgccg
     4261 cagagatact gccggaaaac ctgaaagatg cttggaagga caagatcgca tccggcctat
     4321 ccgtagccac ggcgctctcc atgaaggcgg gcaaaactct tccgtggaaa acgataaagg
     4381 atgcgattaa cagcgcgctt caggcacgat tcctgaaact agcatctgat tcgcagactt
     4441 ggccgtgtga tttcccgtcg gcccaattcg tcaagctcac agaaagcagt gacccaccgg
     4501 acgaagcggg caaagacgat ttttggcgag aagatgacaa gaaacttgtc gctgcggcca
     4561 ccctcgaacc gtcacagatt caggatctcg gcgacgtcat gcccaagctg ctggaactca
     4621 ggtccaagtc caatactccg ctccaattcc acatccaaat cgagatgggc gacggcatga
     4681 caaagcccgc cgaagagacc gtccagaagg ccaacgccat cctgaaaagc gtcaacgagg
     4741 ggctggaatt tcatttttga cccctgtcac cattgctcgg ctcagttgcc aaacacatag
     4801 accaagttga ggcggtcggc gactgcg
//
LOCUS       DAOSLE010000179         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_15785_length_4798_cov_8.685431, whole genome shotgun sequence.
ACCESSION   DAOSLE010000179 DAOSLE010000000
VERSION     DAOSLE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15785_length_4798_cov_8.685431"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..2294
                     /locus_tag="PK576_09705"
     CDS             303..2294
                     /locus_tag="PK576_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61904.1"
                     /translation="MTMHETNDLRLLAEEENGMSARPGRPSLLHKTGKLLFCGLTAMG
                     LAGALSARGDLYVNIATGNDGNDNPNSAERPYKTLQRAITRLNELKVDGVVIHVAPGT
                     YAPANSTFHGHEFPRLNFSVVGEGTAENPVVIDGGGSNACFRITNSKKIGAFSNIIFR
                     NGLHYSGGGIGCLAGWTTSGVSGVTNCVFENCHSTNGCGGAVMSYGYTTSFSNCTFRN
                     CSAVRVDNGSWSGQVKNGCGGAVMLYAVKGDCDFTACSFETNAASGGGGAVMMQVDDA
                     PGGTMEHSDIGLRLTDCTFVGNTSGGQGIVNGIVRSCERTAFTGNSASWGSVWFLDGY
                     LGTKYGGAYFVTNRFVNCTFTDNLGTNVGCFGWSYSKRFEFEHCTFAGNTSLNGAAVL
                     QTDGHSPHCFFRDCTFSRNAGEGVLAVTSHNGANFTLELARTVFTENDAGAKPLFQNL
                     SASRILNSCFSANCGGSVLDFASGGGGVYHTNTLRNCLLDHNTNTVNEGILRIFWTLA
                     ETADVCNNTFVDNVATDQPSALWINSVASYEDLGCHYANNLFFANRKTDGTSVKQTAG
                     VFNSFATNNWIEVGGTTLADGTRGNIVGVDPKFTNRADGDYTISAASACRDCGAFFEW
                     MTGACDIRANPKYPRVTGAAPDIGCYEWLPERYLTVIVR"
     gene            2312..4612
                     /locus_tag="PK576_09710"
     CDS             2312..4612
                     /locus_tag="PK576_09710"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOE61905.1"
                     /translation="MDWFLLFLALAVPVVAVGETRFNGMKYFGAPGDWISVRLDGFTT
                     EELPALFENDGVRQVIVRHEVPNIAGVHGPKGFPFPNWGDLTVTLRSWDGAFSRVLGT
                     QKTGCWGFGQERRFPLSFKGLKPGAYLVVAEMPPHIRAEGVFNPTNTGSGAIGLPMLY
                     GRNAMRFAIYPKTDPHRVFGVGNRMIHTGKWWTGYQLANTLEARDLQPVCVSDGDSFQ
                     NAILGAYRVEHTTVDSAGPTNVPHLNNPVARMLDIFSPTGRVELVRRGEETGRRLAKD
                     AGVLALALGGEKPSFNAGRVCPTEWADADFRAFCRARYTNDLAAANRVWGRTFTDWRE
                     VRQPIYGVQSAATEKTGAAAIDWYANMGNMSDEFVKEINRPENVEFAMDWYRWITKAS
                     IEMYATYADAARRHDRKTLYTNNYCWPNFFAHLCLPTWRRLDAAALDFQYVCTFPRTL
                     GSNSEMIEIAEMTESAMGNKPIYGFEVYVQPHYPAEMAALQNWAMVAHGMRVNLVFAW
                     KPYSDYGGKVFENGPKAWTRPADAKPMWMIIDTDGTRLPCYAAVKRSSEEIAAWHSQF
                     DALSVRRTRGRTAVYLSDETGMYTMLRTGNRPYLENHLCHARNRIASGLRLGGVRIEY
                     LDDETLEKLVRRDFDAVVLPPTPRIAPESARRLDAFERAGGEVIRLDDWSADFFDRYP
                     QIPRSAYWRAPDGRVNDVVVVVRTQEGTGRRFVFVLNRTGEAVEGELAGRDFTEGQTF
                     ADTLRGGKVGRSFRLDGWGYRVLLCD"
BASE COUNT          927 a         1414 c         1520 g          937 t
ORIGIN      
        1 ataatttgac aggattacag gattttcagg attgaacaac atcatggtaa tcatgtccta
       61 aacttacttc caaggttttt cgcggcggag ccgcgcggcg gactaaatcc taaatcctaa
      121 ctcctaaata aatcctacat ccgcgcgtag cgcggaagac gcccgcgctc ccgactgcgc
      181 acacaaaaac cacgcactaa actcgcatgc ccctgtcacg aaccgattac agagcaagcc
      241 caattgacgc aacgggtcag gggtgatatt gtaaatcgta agtccaacaa aggggaacac
      301 acatgactat gcacgaaacc aatgacctgc ggctgctggc tgaagaggaa aacggcatgt
      361 cagcgaggcc ggggcgcccg tccctgctcc acaagaccgg aaaacttctg ttctgcggat
      421 tgacggcgat ggggttggcc ggtgcgttat ccgcacgcgg cgatctctac gtgaacatcg
      481 cgacgggcaa cgacgggaac gacaacccga attctgcgga gaggccgtac aagacactgc
      541 aacgggccat cacgcgcctg aacgagctca aggtggacgg tgtcgtgatc cacgtcgcgc
      601 ccggcacgta tgcgccggca aactcgacct tccatggcca tgaatttccg cggctcaatt
      661 tctccgttgt cggcgaaggg accgcggaga accccgtcgt gattgacggc gggggttcga
      721 atgcctgttt ccgcatcacg aattcgaaaa agatcggcgc gttctccaac atcatctttc
      781 gaaatggtct ccactattcc ggcggcggta tcggctgtct ggcgggctgg acgacgtccg
      841 gtgtctcagg ggtgacgaac tgcgtgttcg agaactgcca ctcgacgaac ggctgcggcg
      901 gcgcggtgat gtcgtacggc tatacgacgt ccttttcaaa ttgcacgttc cgtaattgct
      961 cggcagtacg tgtggataac gggtcgtggt ccggacaggt taagaacggt tgcggcggtg
     1021 cggtgatgct ctacgccgtg aagggtgatt gtgacttcac ggcgtgttca ttcgagacga
     1081 atgcggcgtc cgggggcggt ggagcggtga tgatgcaggt ggatgacgcg cccggcggga
     1141 cgatggagca ttccgacatc ggcctgcgtc tgacggactg cacctttgtc gggaacacgt
     1201 cgggcggaca aggcatcgtg aacggcatcg tccgttcgtg cgagcggacg gccttcaccg
     1261 gcaactcggc ctcctggggc agcgtttggt ttttagatgg ttatctgggg acgaagtacg
     1321 gtggcgccta ctttgtcacg aatcgcttcg tgaactgcac attcacggac aatctcggta
     1381 ccaacgtcgg ttgcttcggg tggagttact cgaaacgttt tgaattcgag cactgtacgt
     1441 tcgctggcaa cacctcgttg aacggcgccg ccgtgctcca gactgatgga cattcgcctc
     1501 actgtttctt ccgggactgt accttcagcc gcaacgccgg cgagggcgtc ttagcggtca
     1561 cgtcccacaa cggggcgaac ttcaccctcg agctcgcgcg gaccgtgttc acggaaaacg
     1621 acgccggggc gaagccgctg ttccagaatt tgtcggccag ccgcatcctg aactcctgtt
     1681 tctccgccaa ctgtggaggc agcgtacttg atttcgcgtc cggaggcggc ggggtctacc
     1741 acacgaacac attgcggaac tgtcttctcg accacaacac gaacaccgtc aacgagggca
     1801 tactgcggat tttctggacg cttgcggaga cagcggacgt ctgcaacaac acgttcgtgg
     1861 acaatgtcgc gacggaccag ccctccgctc tctggatcaa cagcgtagcc tcctacgagg
     1921 atcttggctg ccactacgcg aacaacctct tcttcgcgaa ccgcaagacg gacggcacgt
     1981 ccgtgaaaca gacggccggc gtcttcaact cctttgcgac gaacaactgg atcgaggtgg
     2041 gcggcacgac gctcgcagac ggcacgcggg gtaacatcgt tggcgtggac ccgaaattca
     2101 cgaaccgcgc ggatggcgac tacacgatca gcgcggcgtc ggcctgccgg gactgcggcg
     2161 cctttttcga gtggatgacg ggtgcatgcg acatccgggc aaacccgaag tacccgcgcg
     2221 tgacgggcgc ggcgcccgat atcgggtgct atgagtggct gcccgagcgt tatctcacgg
     2281 tgattgtccg ttgaacgtcc ggaggatcgg gatggactgg tttcttcttt tcctagccct
     2341 cgccgtgccg gtcgtcgcgg tcggcgagac gcgcttcaac ggcatgaagt acttcggcgc
     2401 gccaggggac tggatttccg tacggttgga tggcttcacg accgaggaac tgcccgccct
     2461 ttttgaaaac gatggcgtgc ggcaggtgat cgtgcggcac gaggtcccga atatcgcggg
     2521 cgtgcatggc ccgaaaggtt tcccgttccc gaactggggc gatctcaccg tgacactgcg
     2581 gagttgggac ggggcgttct cccgcgtcct tggaacacag aagaccggct gctggggctt
     2641 cggccaggag cgtcgctttc cgctctcctt caagggcctg aagccgggcg cttacctcgt
     2701 tgtcgcggag atgccgccgc atatccgcgc ggagggagtc ttcaacccca cgaataccgg
     2761 tagcggggcg atcggcctgc cgatgcttta cggacgcaac gcgatgcgct tcgcgattta
     2821 tccgaagaca gatccgcacc gggtgttcgg cgtggggaac cggatgatcc acacgggcaa
     2881 gtggtggacg gggtatcagc tcgcgaacac gctcgaggcc cgtgacctgc agcccgtctg
     2941 tgtaagcgat ggggattcct tccagaacgc gattctgggg gcgtaccgtg tggaacatac
     3001 gaccgtcgac tcggccggcc ccacgaacgt gccgcacctg aacaacccgg tcgcgcggat
     3061 gctcgacatc ttctcgccga ccgggcgggt cgaactcgtc cgccgcggcg aggagacggg
     3121 acgccgcctc gccaaggacg ccggtgtgtt ggcgcttgca ctggggggcg agaagccgag
     3181 cttcaacgca gggcgggtgt gtcccacgga atgggcggat gccgacttcc gagccttctg
     3241 ccgcgcacgc tacacgaacg accttgccgc cgcaaaccgc gtgtggggac gcaccttcac
     3301 ggactggcgc gaggtgcgtc agccgatcta cggcgtacag tccgccgcaa ctgaaaagac
     3361 gggcgccgcc gcgatcgact ggtacgcgaa catgggcaac atgagcgatg agttcgtgaa
     3421 agagatcaat cgacccgaga acgtcgagtt cgcgatggac tggtaccgct ggatcacgaa
     3481 ggcctcgatc gagatgtacg ccacgtacgc ggatgcggcg cggcggcacg accgcaagac
     3541 gctctacacg aacaattatt gctggccgaa cttcttcgcc catctctgtc tgcccacctg
     3601 gcggcgtctg gatgcggccg cgctcgactt ccagtatgtc tgcaccttcc cgcgtacgct
     3661 cgggagtaac agcgagatga tcgagatcgc cgagatgacg gagtccgcga tggggaacaa
     3721 gcccatctac ggtttcgagg tctacgttca gccgcattac ccggccgaga tggcggcgct
     3781 ccagaattgg gcgatggttg cgcacgggat gcgtgtgaac ctcgtcttcg cctggaaacc
     3841 gtattcggac tacggcggca aggtgttcga gaacggcccg aaggcctgga cgcgtcctgc
     3901 ggacgcgaaa ccgatgtgga tgattatcga cacggacgga acgcgtctgc cctgctatgc
     3961 ggccgtaaag cgttcaagcg aggagattgc cgcctggcat tctcaattcg atgcgctcag
     4021 tgtccgccgt acgcgcggcc gcacggctgt ttacctttcg gacgagacgg ggatgtacac
     4081 gatgttgcgg acgggaaacc gcccctacct cgagaaccac ctctgccatg cgcgtaatcg
     4141 aattgcgagc gggctccgtc tcggcggtgt ccgcattgag tacttggacg acgagacgct
     4201 cgaaaaactg gtccgccggg acttcgacgc cgtcgtactg ccgcccacac cgcggattgc
     4261 gccggaatcc gcacggcgcc tggacgcatt cgagcgtgcc ggcggcgagg tgatccgcct
     4321 cgacgactgg tccgccgact tcttcgaccg ctatccgcag attccgcgca gcgcctactg
     4381 gcgtgcgccg gatggacggg tgaacgacgt ggtggttgtg gtacgcacgc aggagggcac
     4441 gggccgccgt tttgtcttcg tgctcaatcg cacgggcgag gcggttgagg gtgaactcgc
     4501 gggccgagac tttacggaag ggcagacctt cgccgatacg ctccgtggtg ggaaggttgg
     4561 tcgttccttc cgtcttgacg gctggggcta tcgtgtgctc ttgtgcgact gatcgtgttt
     4621 tgcaacggac aatgaatatt tcccaaggcc gatcttctcc tttcgggatc gatgaagacc
     4681 tgaaaaacgg ggtaagtcca atcttgtttc ccctgacggg atgagttgtt ttgcttttgg
     4741 gattcccgac cgcaaggagc tgctgccctg gttcacggag cgcttcgcgc cggtctac
//
LOCUS       DAOSLE010000180         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_15818_length_4788_cov_7.867103, whole genome shotgun sequence.
ACCESSION   DAOSLE010000180 DAOSLE010000000
VERSION     DAOSLE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15818_length_4788_cov_7.867103"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4109)
                     /locus_tag="PK576_09715"
     CDS             complement(<1..4109)
                     /locus_tag="PK576_09715"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOE61906.1"
                     /translation="MKTTDMKTGIKKSVLPWVVCGVLWAGVTARAQYFPPSNGDLIGV
                     LTSVSITNKTLSNVGTAIPIELRLWGHFMLTNTAVGPIAMPRLRMNLAAEEEAWATLE
                     DPADVQRFTDGVGQKTRVTFKYRIREGDMVSKLKISGSDGSDVLGVPYGFDWCNDTWK
                     IVRVSDPASNAVWRFRTTSPRYLELPGDDPTHYDFSYRSVGIKTLQFSSAPSPVQVGE
                     SVTWAVSTVNPVSSGRTVSFYVWVSNTVSQGDVNLNGVKSQKLMIAGGSTTATFQITG
                     LTVGTNTVFLQGTQDYDNNASLGVQNAETRQIVVAPAPEPYVSVLLPNAPAPHNEQTL
                     PETSAFGTGALQVRLSQVYTEDVWVRLDTTLNGGPQSNNVTFSETPKFVRVRAGNHNS
                     DTVAFNVPDGIHASELFGITIMPVITNAAIDAVYSGESVPCIVKVANVAPVVQTPQNG
                     ATATAKRGTPQRFTWSVSDVAADLTGMTVTWDFGDGSALVTTNGYFGEIFHTYTSNGS
                     REVKVFATDKDGKDSAVITMTVTVEDPVPAPKLSLLFSTGTTNTASYTETATPLTGWV
                     RLVLSESFYESVQISLKALPDGQGNLLLGTTNLITIANGDLESGNIPFSLLDGTPDSS
                     VNGITLDPVIVTPNASNYYTDVRQGTVRVQNVVPVIMEPVGYATVAEATTNAAVAPYD
                     AIPMGRSFGFSWALDDVVADSSGLVLDWDFGDNTSQSVTNGLIGKVFHTYSSLGEKIV
                     RLQVTDKDGGKTTRIAFKVNVVPPPPEPTVYVRPPAGYLTETTVMGSGSIDVELSEAF
                     TEKVTVRLAVSPSASDTNGEILLATNLVEFRVGEVFKTVKVSARDGTVASEINGFTVT
                     PSVIGTAAAINHYTELWPGEVQVVNADPVIRTPLDAPDSTTISYTIPQGTGWKFFWDV
                     QDVPADLPGMKVTWYFGDGSSETLTGGSGSVTHTYNAPGIMSVRVVAKDKDGGSDEVN
                     FKVEVRPAKSVIVTPIGPNLEANYWGAPGLGNGMVFSEGALGRINRNNTYFFTYGPGA
                     SSAALRAVPYKTGPSGWYTVTNFNNEGAAIPGAQHHFDSFFYVWVGEDQGLPAQDIKP
                     SQASPTTALTLPASAGGTESVDIRIIQAIFSREYRAADNCGDINQDGIPDKIAAQYNL
                     PVIARGGTDGGGGAGSTGEMPIELTPVGDFNRDKDAAGNEVGDFLPGAASGGGAIIGG
                     ISNVFAAVGDPFTAFLEVRGFHPGLNSALFNSDDDFGENETSRDCERPTNPTLQDTDG
                     DLFPDGWEYYFWYNAKVNGVTGMAYTPTNIAVGTLIPTRTITTAFDPIVPATDSATGA
                     AVNRDLDNDGLSDIEELTIGTNPIHWDTDGDGMCDGWEVMRGLNPNDSR"
     gene            complement(4158..4571)
                     /locus_tag="PK576_09720"
     CDS             complement(4158..4571)
                     /locus_tag="PK576_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61907.1"
                     /translation="MNGCSKRRPPAEEALPEVITNRMADAAYRAELAELRREQTRLAG
                     GRAEVVEQMRVMVERARATLPEGADDAAVKAVLVKDPEWNRLENANQEWIGKIQDNLA
                     QARERVRQRMLAEERDNRRVAEGTARPAAGETAAP"
BASE COUNT          851 a         1545 c         1469 g          923 t
ORIGIN      
        1 tcacgcgaat cgttcgggtt caagccgcgc atcacctccc agccgtcgca catgccgtcg
       61 ccgtccgtat cccagtggat cgggttcgtg ccgatcgtca gctcctcgat gtcgctcagc
      121 ccgtcgttgt cgagatcgcg gttgaccgcc gcgccggtcg cggagtcggt cgcgggcacg
      181 atcgggtcaa aggcggtggt gatggtgcgg gtcgggatca gcgtgccgac cgcaatgttg
      241 gtgggcgtgt aggccatgcc ggtgacaccg ttgaccttgg cattgtacca gaagtaatac
      301 tcccatccgt ccgggaagag gtcgccgtcc gtatcctgca gggtcgggtt ggtgggccgc
      361 tcacagtcgc ggctggtctc attctcgccg aaatcgtcgt cggaattgaa caacgcgctg
      421 ttgagtccgg ggtggaagcc gcgcacctcc aggaaggctg tgaacggatc gccgaccgcg
      481 gcgaagacat tggagatccc gccgatgatg gcgccgccgc cgctcgccgc gccgggcagg
      541 aagtcgccga cctcattgcc ggccgcatcc ttgtcgcggt tgaagtcccc gacgggcgtg
      601 agttcgatcg gcatttcgcc ggtcgagccg gctccgccgc cgccgtcggt gccgccacgc
      661 gcgatcacag gcaggttgta ctgggccgcg atcttatcgg gaatgccgtc ctggttgatg
      721 tcgccgcagt tgtcggccgc acggtactcg cggctgaaga tcgcctgaat gatgcggatg
      781 tccaccgact cggtgccgcc tgcggaggcc ggcagggtca gcgccgtggt gggactggcc
      841 tgtgagggtt tgatatcctg agccggcagg ccctgatcct cgccgaccca cacatagaag
      901 aaactgtcaa agtgatgctg ggctcccgga atcgcggcac cctcattgtt gaagttcgtc
      961 acggtatacc atccggaggg gccggtcttg tacggcacgg cgcgcagcgc cgccgacgag
     1021 gcaccggggc cgtaggtgaa gaagtaggtg ttgttgcggt tgatgcggcc gagggccccc
     1081 tccgaaaaga ccatcccgtt gcccagaccc ggcgctcccc agtagttggc ctccaaattg
     1141 gggccgatcg gcgtgacaat cacactcttg gcgggcctga cttccacctt gaagttcact
     1201 tcgtcgcttc cgccatcctt atctttggcc accacacgca cggacatgat gccgggggcg
     1261 ttatacgtgt gcgtaacgga acccgaaccg ccggtgagcg tctcgctgga accgtccccg
     1321 aaataccagg tcactttcat gccgggcagg tccgcgggca catcctgcac atcccagaaa
     1381 aacttccagc ccgttccctg cggaatggtg tacgagatcg tggtggagtc cggagcgtcc
     1441 aacggggtcc ggataaccgg atcggcattg acgacctgca cttctcccgg ccacagttcg
     1501 gtataatggt tgatggccgc cgccgtaccg atcaccgacg gcgtgacggt gaacccgttt
     1561 atttccgacg ccacggtgcc gtcgcgcgcg ctgactttga cggttttgaa gacttcaccc
     1621 acgcggaatt cgaccagatt ggtcgccagc aggatctccc cgttggtgtc actggcagac
     1681 gggctgaccg ctaaccgcac cgtcaccttt tccgtgaagg cctcggagag ttcgacatcg
     1741 atcgagcccg aacccatgac ggttgtttcc gtgaggtacc cggcgggcgg gcggacatag
     1801 accgtgggtt ccggcggggg cgggaccaca ttcaccttaa aggcgatgcg cgtggtcttg
     1861 ccgccatcct tgtccgtaac ctgcagccgg acgatcttct cgccgagcga gctataggtg
     1921 tggaacacct tgccgatcag gccgttggtc acgctctgcg aggtgttgtc gccgaaatcc
     1981 cagtccagaa ccagaccgct ggagtcggcc accacatcat caagcgccca ggagaatccg
     2041 aacgaccggc ccatggggat cgcgtcgtag ggggccacgg cggcattggt ggtggcctcg
     2101 gccacggtgg cgtatccgac cggctccatg atcacgggaa ccacgttctg cacgcgcacc
     2161 gttccctgac gcacgtccgt atagtaattg gaggcgttgg gcgtgacgat gaccggatca
     2221 agcgtgatcc cgttcacgct gctgtcgggg gtgccgtcca gcaggctgaa gggaatgttg
     2281 ccggactcca gatcgccgtt ggcgatggtg atgaggttgg tggtgcccag caacaggttg
     2341 ccctggccgt ccggcagggc cttcaggctg atctgtacac tctcgtagaa ggactctgaa
     2401 agaaccagcc gcacccagcc ggtgagcggg gtcgccgtct ccgtgtaaga ggcggtgttg
     2461 gtggtgcccg tcgagaacag cagggagagt ttcggagccg ggacgggatc ctcgaccgtg
     2521 acggtcatcg tgatgaccgc ggaatcctta ccgtccttat ccgtggcgaa aaccttcact
     2581 tcgcgcgaac cgttgctggt gtaggtgtgg aagatttcgc cgaagtaccc gttggtggtc
     2641 accagtgcgg acccgtcgcc gaaatcccag gtgacggtca tgcccgtcag atcggccgcc
     2701 acatccgaga cgctccaggt gaagcgttgc ggcgtgccgc gcttggccgt ggccgtggcg
     2761 ccgttctggg gcgtctgaac cacaggcgcg acattggcca ccttgacgat gcacggcacg
     2821 ctttcgccgc tgtacacagc gtcgatcgcg gcattggtga tcaccggcat gatcgtgatg
     2881 ccgaaaagtt cgctggcgtg gatgccgtcc ggcacgttga acgcgaccgt gtccgaattg
     2941 tgattgcccg cgcgcacgcg gacaaacttg ggcgtctcgc tgaacgtcac gttattgctt
     3001 tgcggaccgc cgttgagcgt ggtgtccagc cgcacccaaa catcttccgt atagacttgc
     3061 gacaaccgca cctgcagggc accggtgccg aaggccgagg tttcgggcag ggtctgctca
     3121 ttgtgcggcg cgggggcgtt cggcagcaga accgagacat acggttccgg ggccggggcc
     3181 accacgatct gccgggtctc ggcgttttgg acgccgaggc tcgcgttgtt gtcgtaatcc
     3241 tgcgtgccct gcaggaaaac cgtgttggtg ccgaccgtga ggccggtgat ctggaaggtg
     3301 gcggtggtcg aacccccggc gatcatcagc ttttgggatt taaccccgtt caggttcaca
     3361 tccccttgcg acacggtgtt ggaaacccac acgtaaaagc tcaccgtgcg tccggacgaa
     3421 accggattga cggtggaaac ggcccacgtc accgactcgc cgacctgcac cggcgagggc
     3481 gcggaactga actgcagggt tttgatcccc acggagcggt aactgaaatc ataatgcgtc
     3541 ggatcatcgc cgggcagttc gagatatcgg ggcgacgtgg tgcggaaacg ccagaccgca
     3601 ttgctggccg gatcactcac gcgcacgatc ttccaagtgt cattgcacca atcgaacccg
     3661 tagggcacac cgagcacatc gctgccgtcc gaacccgaga tcttgagctt cgagaccatg
     3721 tccccttcgc ggatccggta cttgaaggtg acgcgcgtct tctgacccac gccatccgtg
     3781 aaccgctgga catccgccgg atcctcaagc gtggcccacg cctcctcttc cgccgccaga
     3841 ttcatgcgca accggggcat ggcaatcggg ccgaccgccg tgttcgtgag catgaaatgg
     3901 ccccacagac gcagttcgat cggaatcgcg gtgccaacgt tgctgagcgt tttgttcgtg
     3961 atactcaccg aggtgagcac cccgatcaga tctccgttcg agggcggaaa gtactgggcc
     4021 cgggctgtca cgcctgccca caaaaccccg caaaccaccc aaggcaacac cgactttttg
     4081 attcccgttt tcatatccgt tgttttcatg cctcgtctcc tgtccatgcg aaaaacatac
     4141 tctcccgtcc acacgggtta cggtgccgca gtctctcccg cggccggccg ggccgttccc
     4201 tcggccaccc tccggttgtc acgctcctcg gccagcatcc gctgccggac ccgctcccgg
     4261 gcctgagcca gattgtcctg aatcttgccg atccactcct gattcgcgtt ctcaagccgg
     4321 ttccactccg gatccttcac cagcaccgcc ttgaccgcgg catcgtcggc tccctccggc
     4381 aacgtcgcgc gcgcgcgctc caccatcacc cgcatctgct caaccacctc ggcgcgcccg
     4441 cccgcaagcc gcgtctgctc tctccgcagc tccgcaagct cggcgcgata cgccgcgtcc
     4501 gccatccggt tcgtgatgac ctccggcaga gcctcctcgg cgggcggccg acgcttcgaa
     4561 cacccgttca aaaaaaagag ggaaaaaacg attgacagga atgccgttcc taagcggacc
     4621 gccacaccac actctcggat tttccaaccg ttacactcaa aagctatccg cattccactc
     4681 gcttcccggt ccgttcaaac cgctgcaacc ataccctgtc acgccaaggg cggactatgc
     4741 cgcgattgtt atagcttacc atgcccaaaa gaggtcgggc aagcccta
//
LOCUS       DAOSLE010000181         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_16008_length_4741_cov_6.909091, whole genome shotgun sequence.
ACCESSION   DAOSLE010000181 DAOSLE010000000
VERSION     DAOSLE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16008_length_4741_cov_6.909091"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PK576_09725"
     CDS             complement(<1..1243)
                     /locus_tag="PK576_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOE61908.1"
                     /translation="MADIIGFDNDTYLTEQTEEIKRRAEKSGNKLYLEFGGKLMHDMH
                     AARVLPGYDPNVKLRLLQKLKDKAEIVLCVFAGDIERKKMRADFGISYDTDALKMIDG
                     LRSWGLLIRAVVITRFCGQHAAKQFRARLEHRGIKVYYHYVTQGYPANVDTIVSPEGY
                     GANEYIETERPIVVVTGPGPGSGKLATCLTQLYHDRNAGRLAGYAKFETFPVWNLPLE
                     HPINIAYEAATVDLNDANMVDPYHLQAYQKVAINYNRDIDAFPLLRAIWEKITREPCP
                     YKSPTDMGVNRVGFGITDEAVVCAAAKQEVIRRYFRHLCEFSSGQTEHITVDRIELLM
                     QKLDLVPEDRVVVTPARQAGQDAMKKGKGRNGIFCGAALQLKDGRIVTGKNSEQMHAA
                     ASMVLNAIKELSGIPDQIHLIA"
     gene            1606..2376
                     /locus_tag="PK576_09730"
     CDS             1606..2376
                     /locus_tag="PK576_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOE61909.1"
                     /translation="MKTLIAVLLCSGCLAYARAAQTACEMTVAEVFTDADGGTLPYRI
                     YLPAGIPAERKLPLVLFFHGAGERGTNNVSQLVHGVMPLIRYGQNAKDPAILLVPQCP
                     NGMQWVSVPWGDSRHTMPAQPSIPMRRALALLREKIRTLPVDPARVYVTGISMGGYGT
                     WDAIQREPDLFAAAIPVCGGGDTACAPRIRHIPIWTFHGEKDGAVPVCRSRDMAEALK
                     SCGGNVRYREYPGAGHGVWTPTYNDTEVLTWFFSQRKP"
     gene            2469..2657
                     /locus_tag="PK576_09735"
     CDS             2469..2657
                     /locus_tag="PK576_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61910.1"
                     /translation="MLLPTLPACLPELPISAYAGEIAAALRTHPVVIVSGDTASGKTT
                     QVPVSAKRLAALWKRVFA"
     gene            2722..3390
                     /locus_tag="PK576_09740"
     CDS             2722..3390
                     /locus_tag="PK576_09740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOE61911.1"
                     /translation="MTHTHRRLTLEFAALYLVLPVFLFLIRHQGIPLPVLPALWCAAG
                     PAAYCLVRRHNWDLRAFFALPASARRIGTLALRAALAGAVLTGTLWLTAPDSLFELPR
                     RNPRLWAIVMTAYPVLSVFPQGILYRALFFTRYAVLFQNRRAAWLASAVVFSLAHLLF
                     NNFWALAWTLLGGLLINRSYLRHGSLMAAAAEHAVYGQCLFTCGWGRFLYHGTTGLAE
                     SLIP"
     gene            complement(3510..4547)
                     /gene="pheS"
                     /locus_tag="PK576_09745"
     CDS             complement(3510..4547)
                     /gene="pheS"
                     /locus_tag="PK576_09745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE61912.1"
                     /translation="MTLAELDSQQADSLAEVASAADAAALEKVRVRYLGRNGLLPAII
                     RQMKEVPAAERPEFGRRVQAWRSALEEALAGRMADAVAAREEQAAFDCTLPGRWPACG
                     AKHPVSRVIEETAAIFARLGFTVADGPDVETRFNNFTALNTPAHHPSMDASDTFWLTD
                     DRLLRTQTSPVQIRMMTCHTPPVRIITPGRTYRRDTTDATHSANFHQIEGLYVDTKPV
                     SLADLKSTLSYYAREMMGAKAGVRFRPHFFPFTEPSVEVDFSCHVCGGKGCRICKMSG
                     WIEIAGAGMVDPRVYEHVNRARGDMAYDPATVYGYAFGFGVERIAMVKYGIPDIRWLY
                     ENDTRFLSQLA"
BASE COUNT          837 a         1503 c         1411 g          990 t
ORIGIN      
        1 gggcgatcag atgaatctga tccggaatgc ctgagagttc cttgatcgcg ttcagaacca
       61 tgcttgccgc cgcgtgcatc tgctcggagt tcttgcccgt gacgatccgt ccgtccttga
      121 gttgcagggc tgcgccgcag aatatcccgt tgcgtccctt gccttttttc atggcgtcct
      181 gtcccgcttg gcgcgcgggc gtcacgacca cgcggtcctc cgggaccaga tccagttttt
      241 gcatcaacag ttcaatgcgg tccacagtga tgtgttcggt ctgtccggag gagaactcgc
      301 agagatggcg gaagtagcgg cggatcacct cctgtttggc tgccgcgcag acgaccgcct
      361 cgtcggtgat gccgaagccc acgcggttga cgcccatgtc ggtcggtgat ttgtagggac
      421 agggttcgcg ggtgattttc tcccagatgg cacgcagcag cgggaaagca tcgatgtcgc
      481 ggttgtagtt gatcgccacc ttttggtagg cctgaagatg atagggatcc accatgttgg
      541 catcattgag atcgaccgtg gcggcctcat aggcgatgtt gatggggtgc tccagcggca
      601 gattccagac cgggaacgtt tcaaatttgg cgtaaccggc caagcgcccc gcattgcggt
      661 cgtggtagag ctgagtaagg caggtggcca gtttgccgct gccggggccc ggtccggtca
      721 cgaccacgat cggccgttca gtctcgatat actcattcgc cccgtaaccc tcggggctga
      781 cgatcgtgtc gacgttggca gggtaacctt gggtcacgta gtgataatag accttgatgc
      841 cgcggtgctc gaggcgggcg cggaactgct tggcggcatg ctgtccacaa aaacgggtga
      901 tgaccacggc acggatcagc aggccccaac tgcgtagacc gtcgatcatc ttaagagcgt
      961 cggtgtcgta agagatgccg aaatccgcgc gcattttttt gcgttcgatg tcgcccgcaa
     1021 agacacagag cacgatctcg gccttgtcct tgagcttttg cagaaggcgt agtttgacat
     1081 tcggatcata cccgggcagg acacgggccg cgtgcatatc gtgcatcaac ttgccgccga
     1141 attcgagata aagcttgttg ccgctctttt cagcgcggcg tttgatttcc tcggtttgtt
     1201 cagtgaggta cgtgtcattg tcaaacccga ttatgtccgc cataaacaac ctgcctccgc
     1261 gcgataagga agagaaacaa aaacggtcaa ccgttaatgc gtggccggga caggactata
     1321 gcgcaaaacc ggtggcgacg taaactcatt ttctgcggac gcgaatccgt cgcggcatgt
     1381 catagtaaaa cctacggtgt cttttctata attttgacgg accgcgtatt gcgaccacgc
     1441 agcggctcgc aacggcgaat gtaaaagtgc tgccctgatt tttcacgcga atcgtcatcc
     1501 tctccgcgca tttgacagat ccgacggcgc gttgctatgc tacgcctcat tcggatccgg
     1561 cacgggatgc ataccgcaaa ccggcccttt ccctccggag aaatgatgaa aactctaata
     1621 gctgttctgt tgtgttcggg gtgtctcgca tatgcgcggg cagcccagac tgcctgcgaa
     1681 atgactgttg ccgaagtgtt caccgatgcg gatggcggca ccctccccta ccggatttac
     1741 ctgcctgccg gaatcccggc ggagcgcaaa cttccgctgg tgctgttttt ccacggtgcg
     1801 ggcgagcggg gcaccaacaa tgtcagccaa ctggtgcacg gcgtgatgcc gctgatccga
     1861 tacgggcaga acgccaaaga ccccgcgatc cttctggtgc cgcagtgccc gaacggtatg
     1921 cagtgggtaa gtgtcccgtg gggagattcc cgccacacca tgccggcgca gccgtcaatc
     1981 cctatgcggc gcgcgctggc gctgctccgc gaaaagatac gtaccctgcc cgtcgatccc
     2041 gcgcgcgttt atgtcaccgg tatctctatg ggcggatacg gcacctggga cgccatccag
     2101 cgtgagccgg acctgttcgc tgcggccata ccggtctgcg gcggtggcga cactgcctgc
     2161 gcgccacgta tccgacacat cccgatctgg acgttccacg gtgagaagga cggcgcggtg
     2221 ccggtctgcc ggtcgcgtga tatggcggaa gcgctcaagt cgtgcggcgg caacgtgcgg
     2281 taccgtgaat atccgggcgc ggggcacggg gtctggacac cgacgtataa cgataccgaa
     2341 gtgcttacct ggttcttctc gcaacgcaaa ccgtagcctt cccgcaatcg gccggacacc
     2401 gggcctggcc caaactcatc cggcaggtgc ggcggcaacc ccgaaccgtt cggaacaccg
     2461 tatttcccat gttgcttccg actttgccag cctgtcttcc ggaactgccc atctccgcgt
     2521 atgccggcga gatcgccgcg gctctacgca cccatccggt ggttatcgtc agcggcgata
     2581 ccgcttccgg caagaccacg caggttcccg tgtccgccaa acgtctcgcg gccctgtgga
     2641 agcgggtttt cgcgtgaccg cccggatctt gacaccccgg ttcccctccg gcatcatgcc
     2701 ccggttccgg aaaggctcgc catgacacac acacaccgtc gtttaacgtt ggaattcgcg
     2761 gccctctatc tcgtgctgcc ggttttcttg tttctcatcc gccatcaggg gattcccctg
     2821 ccggtactgc cggctctttg gtgcgcggcg ggtccggcgg cgtactgtct ggtccgtcgg
     2881 cacaactggg accttcgggc ctttttcgcc cttccggcca gtgcgcgccg gatcggcaca
     2941 ctcgcgttgc gcgccgcctt ggccggcgcc gtcctcaccg ggacgctgtg gctgaccgca
     3001 cccgactcgc tgttcgagtt gccgcgacgc aatccccggc tctgggcaat cgtgatgacg
     3061 gcatatcccg tgctttccgt attcccgcaa ggcatcctct accgcgccct gtttttcaca
     3121 cggtatgcgg tgctgttcca aaaccgccgc gcggcctggc tggcgagtgc ggtcgtgttc
     3181 agtctggccc acctgctctt caacaatttt tgggcacttg cctggacttt attgggaggg
     3241 ctgttgatca accgctcgta tctgcggcac ggctcgctga tggctgcggc tgcggaacat
     3301 gccgtctacg gacagtgcct gttcacctgc ggctggggtc gtttcctgta ccacggcacc
     3361 accggcctcg cggaaagcct gattccataa accgccgccg gtccctgccc tggacggacg
     3421 cctcggggat gcgtccctac cgttgcttcg gcgttttcga cgtcgcccgt ctcccttggg
     3481 ttgcgggctc cccccgcatt agatccgcat cacgccaact ggctcagaaa acgggtgtcg
     3541 ttctcgtaga gccagcggat gtcgggaatg ccgtacttga ccatcgcgat gcgttcgaca
     3601 ccgaacccga aggcatagcc gtagaccgtc gcagggtcgt acgccatatc gccccgcgcc
     3661 cgattgacgt gttcgtatac gcgcggatcc accatgcccg cgccagcgat ttcgatccaa
     3721 ccgctcatct tgcagatgcg gcatcccttg ccgccgcaca catggcacga gaagtccact
     3781 tcgacgctcg gctcggtgaa ggggaagaag tgcggacgga aacgcacgcc cgccttggcg
     3841 cccatcatct cccgcgcgta ataggacaac gtagacttga gatccgcaag cgagaccggc
     3901 ttcgtatcaa cgtagaggcc ctcgatctga tggaaattcg cgctgtgcgt ggcgtcggtc
     3961 gtgtcacggc ggtaggtgcg ccccggcgtg atgatgcgca ccggcggcgt gtgacaggtc
     4021 atcatgcgga tctgtaccgg cgaggtctgc gtgcgcagca gccggtcatc cgtcagccag
     4081 aaggtgtcgg acgcgtccat cgagggatgg tgcgcgggcg tgttgagcgc cgtaaagttg
     4141 ttgaagcgcg tctcgacatc cggcccgtcc gcgaccgtga agcccagccg cgcaaagatc
     4201 gcggcggtct cctcgatcac gcgtgagacc ggatgtttcg ccccgcaggc aggccatcgt
     4261 cccggcaacg tgcagtcgaa ggccgcctgt tcttcccgtg ccgcgacggc atccgccatg
     4321 cgtccggcca acgcctcctc cagcgcggag cgccacgcct gcacccgcct gccgaactcc
     4381 ggccgctcag cagccggaac ctccttcatc tgccggatga tcgcgggcag caatccgttg
     4441 cgccccaaat accggacacg caccttctcc agcgcggcgg catccgccgc agaggccact
     4501 tcagccaaag agtccgcctg ttgagaatcc aattccgcaa gagtcatata cccgtaacct
     4561 ttttttgaga atgcttttta gtttaccgcg cctcctcccc gaaaagcaac cgcgttttac
     4621 aggtcacttg gcaaagtaaa tgcggccaaa actgggtctt tatgacgcac ggtcgcattc
     4681 agttgctccg aaaacggcgc ggaacgcgct ccgcgcagcg cggcggcctt cgccggggtt
     4741 g
//
LOCUS       DAOSLE010000182         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_16058_length_4728_cov_8.755831, whole genome shotgun sequence.
ACCESSION   DAOSLE010000182 DAOSLE010000000
VERSION     DAOSLE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16058_length_4728_cov_8.755831"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1697)
                     /gene="cimA"
                     /locus_tag="PK576_09750"
     CDS             complement(102..1697)
                     /gene="cimA"
                     /locus_tag="PK576_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOE61913.1"
                     /translation="MEKIFLYDTTLRDGAQGEGISFSDTAKVRFARLLDDFGIDYIEG
                     GFAGSNPRDRKFFEDIRRERLSHARITAFGSTRRVNLDAASDPQIAGLLAADTEWVTI
                     YGKAWLLHVRDVLRATGPQNLQIIADTVGHLRQNGRQVFFDAEHFFDGYKDDPAYALQ
                     ALDAAVREGAAAIVLCDTNGGTLPHEIFTITGEVCAAFPGVEVGIHTHNDSNLAVAAT
                     LEGVRGGARQVQGSVNGYGERAGNANLISILPALELKMGYACVGPERLKKLCEVSRIT
                     DDLANQRPDPKQPYVGRSAFSHKAGAHVNAIQKNPRTFEHIAPEAVGNSRHVLVSELA
                     GVANVQIKAAELGIDLAALDRSQIREILNEIKSRENKGYSYESADGSFKILLQKVLNT
                     HKPFFELEGFRVIVEKRGSGEPCLSEATVKVKVRGETELTAGEGLGPVDALNAALRHA
                     LNRFYPEIEQITLTDYRVRILDPQEATRAVTRVLIESGDGTRHWGTVGVSENIIEASW
                     QALLDSVEYKLYEDRAIQIPPAP"
     gene            complement(1697..3049)
                     /locus_tag="PK576_09755"
     CDS             complement(1697..3049)
                     /locus_tag="PK576_09755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE61914.1"
                     /translation="MKVCKFGGSSVANAEQVNKTVDIVLADPERRIVVVSAPGKRDKE
                     DTKVTDMLIACAELALAEKECETPLAAIVERYAEIQRDLGLSAGITAEIEKDLRFRLA
                     GDRSHRGSFLDSMKAAGEDNCAKIVAQALQARGVKAAYLNPQQAGLLLTNDYGNAQLI
                     EESYQNLAQALRDRGEIVVFPGFFGYTREGRVATFPRGGSDITGSILAAAVKAEVYEN
                     FTDVDSVYPVDPRLVPEVKAGIPVMTYREMRELSYAGFGVFHDEAVIPAVKAGVPIQV
                     RNTNHPEEPGTLIVQSRRALAGGVVGIASDDGFCNIFVDKYMMNREIGFGRHLLQILE
                     WEGVSYEHMPSGIDNVSVVIRDRNFDSARERRVLDRIRRDLNPDAVLVERGYALIMVV
                     GEGLYYTVGMASKATRALADAGVNIEMMNQGSSEISMMFGVKAEQRAKAVKALYQAFF
                     "
     gene            complement(3143..4447)
                     /locus_tag="PK576_09760"
     CDS             complement(3143..4447)
                     /locus_tag="PK576_09760"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOE61915.1"
                     /translation="MKQIGVGLLGFGTVGAGVADGLQRNRKVMARRLGADVVLKRIAD
                     LDTVTDRGVAIDPAILTPDALSVIHDPEIRIVVELIGGTGVAKELVLAALNAGKPVVT
                     ANKKLLAEHGEEIFSAAERKGVDIYFGASVGGGIPIIRVLREGLAGNEIQSIHGILNG
                     TCNYILTRMEQEGLPFDAVLRDAQQKGYAEADPSLDIDGFDTAHKAMILASLAYGFHV
                     PMTQVLVEGIRNLNGMDVRYAIEFGYRIKLLAVVRRDGDAVEVRVHPTLVPLDHMLAS
                     VSHVFNAAMVRGDLSGDTLYYGRGAGREPTASTVIGDIGDIVRNLMAGKSRYRRGVCL
                     AEDDGRLRMRPAGDISSRYYVRLMVCDKAGSLGVITTLLGRHGVSILAATQKALSGAR
                     DTGYVPVVILTHAARGADIEEALRGIRAAGVLNEEPVKLRML"
BASE COUNT          780 a         1591 c         1464 g          893 t
ORIGIN      
        1 ggtcgcgggg caacattccc gatcccgaca gcggccccgg cgggaagggc aagcgtctgc
       61 ttgcccggac gggcagacgc tcgtccctcc cgtcaaaccc atcacggtgc aggggggatc
      121 tgtatcgcgc ggtcctcgta cagtttgtac tccacgctgt cgagcagcgc ctgccacgag
      181 gcttcgatga tgttctccga cacgccgacc gtgccccagt gccgcgtgcc gtccccggat
      241 tcgatcagca cgcgcgtgac ggcccgcgtg gcttcctgcg gatccagaat gcggacgcgg
      301 tagtcggtga gggtgatctg ttcgatctcg gggtagaaac ggttcagggc atggcgcagc
      361 gcggcgttca gcgcgtccac gggccccaag ccctcgcctg ccgtgagttc ggtctcgccc
      421 cggactttga ctttgaccgt ggcttcggag aggcagggct cgccgctgcc gcgcttctcg
      481 acgatcacgc ggaacccctc cagctcgaaa aagggcttgt gggtgttgag caccttctgc
      541 agcaggattt tgaacgagcc gtccgcggat tcgtaggagt agcctttgtt ctcgcggctc
      601 ttgatctcgt tcaggatctc gcgtatctga cttctgtcca aggcggcgag gtcgataccc
      661 agttcagccg ccttgatctg cacgttggcc acgccggcga gctccgagac cagcacgtgg
      721 cggctgttgc cgaccgcctc gggcgcgatg tgctcgaagg tccgcgggtt tttctggatc
      781 gcgttcacgt gcgcgccagc cttgtggctg aaggccgaac gcccgacgta aggctgtttg
      841 gggtcgggcc gctggttggc caggtcatcg gtgatccgcg agacctcgca gagtttcttc
      901 agccgctcgg ggccgacgca ggcgtatccc atcttcagtt cgagcgcggg caggatgctg
      961 atgaggttgg cgttgcccgc gcgctcgccg tacccgttga cggaaccctg cacctggcgc
     1021 gcgccgccgc ggacgccttc aagggtggcc gcgaccgcga ggttgctgtc gttgtgggtg
     1081 tggatgccga cctcgacgcc gggaaaggcc gcgcagacct cgccggtgat cgtgaaaatc
     1141 tcgtgcggca gcgtaccgcc gttggtgtcg cacagcacga tggccgcggc accttcgcgc
     1201 accgccgcgt cgagtgcctg cagggcgtag gcggggtcat ccttataccc gtcgaaaaaa
     1261 tgttccgcgt cgaaaaagac ctggcgtccg ttttgccgca ggtggccgac ggtgtcggcg
     1321 atgatctgca agttttgggg gccggtggcg cgcaggacat cccgcacatg cagcagccag
     1381 gccttgccgt agatggtgac ccactcggta tcggccgcga gcaggccagc gatttgcgga
     1441 tcgctggccg cgtcgaggtt cacgcggcgc gtgctgccga aggccgtgat gcgcgcgtgg
     1501 ctgaggcgtt cccgccggat gtcctcgaag aacttgcggt cgcgcggatt ggagccggca
     1561 aacccgccct cgatgtaatc gatgccgaaa tcatccagca gccgcgcgaa acgcactttg
     1621 gccgtgtccg agaaggagat cccctcgcct tgggcgccat cgcgcagggt ggtgtcgtag
     1681 aggaagattt tttccattag aagaatgcct gatagagggc cttcaccgct ttggcgcgct
     1741 gctcggcctt gacgccgaac atcatgctga tttcggatga gccttggttc atcatctcga
     1801 tgttgacgcc cgcgtccgcg agcgcccggg tggctttgga ggccattccc accgtgtagt
     1861 agagcccttc gcccaccacc atgatcagcg cgtagccgcg ttccacaagc accgcgtcgg
     1921 ggttgaggtc gcgccggatg cggtcgagca cgcgccgttc gcgggcggaa tcgaagttcc
     1981 ggtcgcggat caccaccgag acgttgtcga tgcccgaggg catgtgttcg taggaaaccc
     2041 cctcccactc caggatctgg agcaggtggc ggccgaagcc gatctcgcgg ttcatcatgt
     2101 acttgtccac gaagatgttg cagaagccgt catcactcgc gatgccgacc acgcccccgg
     2161 caagcgcgcg gcgcgactgc acgatcaggg tgccgggctc ctccggatgg ttggtgttgc
     2221 gcacctggat cggcacgccg gccttgaccg ccggaatcac cgcctcgtca tggaacacgc
     2281 cgaacccggc gtacgaaagt tcacgcatct cgcggtaggt catgaccggg atgccggcct
     2341 tgacctccgg caccagccgc gggtccacgg gatagacgga gtccacatcg gtgaagttct
     2401 cgtagacctc tgccttgacc gccgcggcca agatcgagcc ggtgatgtcc gaaccgccgc
     2461 gcgggaaagt cgccacccgc ccttcccggg tgtagccgaa aaagccgggg aagaccacga
     2521 tttccccgcg gtcccgcagg gcctgggcca gattctggta cgactcctcg atcagttgcg
     2581 cattgccgta atcgttggtc aggagcagac ccgcctgctg agggttgagg tatgcggcct
     2641 tgacgccgcg cgcctgcagg gcctgggcca cgatcttcgc gcagttgtcc tctcccgcgg
     2701 ctttcatgct gtcgagaaaa ctgccgcggt gggaacggtc gccagccaga cggaagcgca
     2761 aatccttctc gatctccgca gtgatgccag cggacagccc cagatcgcgc tggatttcgg
     2821 cgtaccgctc cacgatcgcc gcgagcggcg tctcgcactc tttttccgcc aaggcgagtt
     2881 ccgcgcaggc gatcagcatg tcggtcacct tggtgtcttc tttgtcgcgc ttgccagggg
     2941 cggagaccac gacgatccgc cgttccggat ccgccagcac gatatccacg gttttgttga
     3001 cctgctccgc attggcgacg gatgacccgc cgaatttaca taccttcatc acgctctcct
     3061 caaaccccgc ccgcttcggc aggggcgact ccccgagctg tccgcaacgc gtccggggaa
     3121 cgcgccttgc ccgcccttgc ctttacagca tcctcaattt caccggctct tcgttcagca
     3181 cgcctgccgc gcggatcccg cgcagcgcct cctcgatgtc cgcgccccgc gccgcatgcg
     3241 tcaggatcac caccggcaca tatccggtat cccgcgcccc ggacagcgcc ttctgcgtcg
     3301 ccgccagaat gctcacgccg tgccggccca gcaacgtcgt gatgacgccc agtgaaccgg
     3361 ccttgtcgca caccatcagc cggacgtagt agcggctcga aatgtcgcct gccggacgca
     3421 tccgcagacg gccgtcgtcc tcggcgaggc agacgccgcg ccggtaccgc gacttgccgg
     3481 ccatcaggtt gcgcacgatg tcgccgatgt cgccgatcac cgtgctggcc gtgggttcgc
     3541 ggccagcgcc gcgtccgtaa tagagcgtgt cgccgctcag atcgccgcgc accatggccg
     3601 cgttgaagac atggctgacc gatgccagca tgtggtccag aggcaccaga gtggggtgga
     3661 cgcgcacctc gaccgcatcc ccgtcgcggc gcaccacggc cagcagtttg atgcggtatc
     3721 cgaactcgat cgcgtagcgc acgtccatgc cgttgaggtt gcggatgccc tcgacgagca
     3781 cctgcgtcat cggcacatgg aacccgtagg ccagcgaggc gaggatcatg gccttgtggg
     3841 ccgtgtcgaa cccgtcgatg tccagactcg ggtccgcttc ggcgtaaccc ttctgctgcg
     3901 cgtcccgcag caccgcgtca aacggaagcc cctcctgctc catgcgggtg aggatgtagt
     3961 tgcacgtgcc gttgaggatg ccgtggatac tctgaatctc gttacccgcc agaccttcgc
     4021 gcaacacgcg gatgatcggg atcccgccgc ccacactggc accgaaatag atgtccacgc
     4081 ccttgcgttc cgcggcactg aaaatctcct ccccgtgttc ggccagcagt ttcttgttgg
     4141 cggtgaccac cggcttcccc gcgttgagcg ccgccagcac cagttccttg gcaacgcccg
     4201 tgccgccgat cagctcgacg acgatccgga tctccggatc gtggatcacc gacagggcgt
     4261 cgggagtcag gatcgcggga tcgatggcca caccgcggtc ggtcacggta tcgagatccg
     4321 cgatccgctt cagcaccacg tcggcaccca gccgcctggc catcaccttg cggttacgct
     4381 gcaagccgtc ggccacaccc gcgcccacgg tcccgaaacc caaaaggcct acaccgatct
     4441 gcttcataca catcctgctt tcctgattcc tttttcttta aaaagaattt ttactatagt
     4501 agaaacagcg acccgcttac aagcccgcaa aaaccgccgc aagcggtcag gacgccccgg
     4561 gaggcaccga aaccaaaaaa aacactcccg cagccacagc tgcgggagtg tcgttctttc
     4621 aggagagccc cttacttctt cgcctttttc gcggcaacct tcttcaccgc tttcttcggc
     4681 gcagccttct tggcgggagc tttcttcaca gcggccttct tcggggcc
//
LOCUS       DAOSLE010000183         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_16532_length_4616_cov_8.568516, whole genome shotgun sequence.
ACCESSION   DAOSLE010000183 DAOSLE010000000
VERSION     DAOSLE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16532_length_4616_cov_8.568516"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1555)
                     /locus_tag="PK576_09765"
     CDS             complement(182..1555)
                     /locus_tag="PK576_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOE61916.1"
                     /translation="MNLNRRTFLQNSVCASASVVWGRYSTDTRPPVTPTPAQQTWHDL
                     ELGMFFHFDIPIYKQEWKWRSFTDFPHPDLYQPAKLDTDQWMEAAKAMGARYAIFVAK
                     HCSGFLQWQSDLYPYGVRQSSWEGGKGDVVRRFVDSCRRYGIRPGLYASVSANGYLGV
                     DNPGLVNRGKGGDDAAQARYVAICERMMTELWTRYGDLFEIWFDGGAIPVSKGGPDLV
                     PILKRHQPNAIVFQGPVGTPNLIRWVGNERGVAPYPCWSTAHAGTSEGGTIEKVFGGH
                     PDGNLWVPGECDVPVRNHDWFWKPGGEKRLYSDAHLIEMYYNSVGRNCNLLLNANPDP
                     DGLVPEADFQKYVTFGKEIRRRFDRPVAETRGAGTLVTLSLPQPQPVNHVMLMEEIRH
                     GERIRAYCVEGQVPGGVWQPLCTGISVGHKRIQTFDRTAVSSIRLHVTETVGEPLIRR
                     LAVFDVT"
     gene            complement(1552..2778)
                     /locus_tag="PK576_09770"
     CDS             complement(1552..2778)
                     /locus_tag="PK576_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOE61917.1"
                     /translation="MTDTLRITDLTGRKTVQQTFQSGALHVISRSGLHAAERLLLDHL
                     GSLTPVPAHLLVAGNRTGVAAMAAALRFPDCAVTCHAFDLHHARAIARNLTANAFSCR
                     FCCDPGVTVPDAVRPPTGDGNGSAPVSLACTATLPDGPFDAALFMSTPGSTTGELLLD
                     QLEELHRALAPGGTCLIACETHVPALLKQVKRTFGPPAVLFHHGPLCCLSARKRGALA
                     NPRDFTASFAASLPGGASHTLYSLPGVFCHRRPDTGGLALAEVAARDLKPGARVLDMG
                     CGCGLVGLLLAAHQPDARVTFVDSHSRALAATRRNLDALGLTAESLVLSDSGLAVTGF
                     DLFAGNPPYYSDFRIADCFLRTARAALTPGGVCLTVAKTAHALAERQRELFGAAEILP
                     RRGYGVVRSVQQGEPS"
     gene            complement(2819..4561)
                     /locus_tag="PK576_09775"
     CDS             complement(2819..4561)
                     /locus_tag="PK576_09775"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE61918.1"
                     /translation="MSLCETPLPDACGQELQRLIAEVPDADLSRLVATEPAFRVGGFR
                     PAGKPAVLRARLRQIVTGAGPLPESVRRILARRSRAAALLNLLSPELLAELRHALASL
                     LGDPALRIAMLLDDRVEVRDQAEAWLREADPLPPLKPDQALDRLREQFAPLTDLIGIA
                     PAANQPPTREAWLTQKARMEERLRCLQTEKRRLQGVEDRLARQATLLKKSEAELDLAR
                     QKAEAAEASLRRNTVELETAKAELARETDRRDERLAAALDLALAREFHGWLAHTREVE
                     TEARETDRHADLVAQAEAALQHQREQDRHSGNRAVLGMRAEQLRSVLEEVRDTLAHAL
                     RVTPELQRMESALASEVRRIEQILNPQPPTTPLEKALLAHLHAAPENDLPGLRDLPER
                     FAPLGVLDDAALDRLREAFRKRLSVLQTAVPPPEPHMAERRGPVARLGRALAGEEPAI
                     LLIDGHNVLFGLPSRYAPPRGASLSEAEKRQRLIDDLTRLTSNTPSIRVWLVFDGPTR
                     SDNQAAANVRVTYSGGEGEHRADGVLLDTLRFFKSSSPDTVVFLVSNDHDLCVAAQRL
                     GAIDITVLELGPFF"
BASE COUNT          823 a         1379 c         1587 g          827 t
ORIGIN      
        1 gtgttctgtg gttaaacagt cgatcccgat cccgatagcg atagcgaccc cgatttggat
       61 ggaaccttga aaccctgaaa ccctgaaacc ctgaaaccca gaaaccaaag agagttgaag
      121 gttcaactgc tctttttagg gtaacccgtt tccttcctcc ccgcacggtt gaccgtgcgc
      181 ctcaagtcac atcgaatacg gccagacgac ggatcagcgg ctcgccgacc gtttcggtca
      241 catgcaggcg gatgctggac acggcggtcc ggtcaaaggt ctgaatacgc ttgtgcccca
      301 cggaaatgcc ggtgcagagc ggttgccaga cgccgccggg aacctggcct tccacacaat
      361 aggcgcggat ccgttcgccg tgacggattt cctccatgag catcacatgg ttcacgggtt
      421 gcggctgggg caggctcagc gtgacgagtg tgcccgcgcc gcgggtctcg gcgaccggcc
      481 ggtcaaaacg gcgtcggatc tctttgccga aggtcacata tttctggaaa tccgcctccg
      541 gcacaagccc gtccggatcc gggttggcgt ttagtaacag attgcagtta cgccccacgg
      601 agttgtagta catctcgatc aggtgcgcat cggagtagag ccgcttctcg cctcccggtt
      661 tccagaacca gtcatggttg cgcaccggca catcgcattc gccgggaacc cagaggttcc
      721 cgtccgggtg gccaccgaag actttctcga tcgtgccgcc ttcgcttgtg cccgcatggg
      781 ccgtgctcca gcagggatag ggcgcgacac cccgttcgtt gccgacccac cggattaggt
      841 tcggtgtgcc gaccggaccc tgaaaaacga tggcgttggg ttgatggcgt ttgaggatcg
      901 gcaccaaatc cggaccaccc ttgctgaccg ggatggcacc gccgtcgaac cagatctcga
      961 acaggtcgcc gtagcgtgtc cagagttcgg tcatcatccg ttcgcagatc gccacatacc
     1021 gtgcttgggc cgcgtcatcc ccgcctttgc cgcgattcac aagcccgggg ttgtctacgc
     1081 ctagataccc gttggcggag acggacgcat aaaggccggg acggatgccg taccgtcggc
     1141 aggagtccac aaaccgtcgc accacatcgc ccttgccgcc ttcccacgag gattgcctga
     1201 ccccgtaggg gtagagatcg gactgccatt gcaggaaccc gctacagtgt ttggccacaa
     1261 agatggcgta ccgcgcaccc atggccttgg ccgcctccat ccactggtcc gtgtccagtt
     1321 tggcgggctg atacagatcg ggatgtggaa aatccgtgaa actacgccat ttccactcct
     1381 gcttgtagat cgggatgtcg aaatggaaga acatgcccag ttccagatcg tgccaagtct
     1441 gttgggcagg agtgggcgtc accggcggac gcgtatcggt tgaatatcgc ccccagacga
     1501 cggacgcgga cgcgcagacg gagttttgca gaaaggttct tcggttcagg ttcatgatgg
     1561 ttctccttgt tggacgcttc ggaccacgcc gtacccgcgg cgtggcagaa tttcggctgc
     1621 cccgaacaac tcgcgctgtc gttccgcaag cgcatgggcc gttttggcga ctgtgaggca
     1681 gacgcctccg ggcgtgagag cggcgcgggc ggttcggaga aagcaatcgg cgatgcgaaa
     1741 atcggagtag tagggcggat tcccggcgaa caggtcgaat ccggttacgg ccaggcccga
     1801 atcggagagc accaggcttt ccgcggtgag cccgagggca tcgaggttgc ggcgcgttgc
     1861 ggccagggcg cggctgtggg aatccacgaa cgtgacccgc gcgtccggct ggtgtgcggc
     1921 cagcagcaga ccgaccagac cgcagccaca ccccatgtcc agcacgcgcg cgccgggttt
     1981 gagatcacgc gccgcgactt cggcaagcgc gaggcctccc gtgtcagggc ggcggtggca
     2041 aaagaccccc ggcagcgagt agagtgtgtg ggaggcccca ccgggaaggg aggccgcgaa
     2101 cgatgcggtg aagtcgcgcg ggtttgcgag cgcaccacgc ttgcgcgcgc tgagacagca
     2161 gagcgggccg tgatggaaaa ggactgcggg ggggccgaac gtgcgtttga cctgtttgag
     2221 cagggccgga acatgggttt cgcaggcgat caggcaggtc ccgccgggcg cgagggcgcg
     2281 atggagttct tcaagctggt cgagcagcaa ctcgccggtt gtgcttcccg gcgtggacat
     2341 gaagagcgcg gcgtcaaacg ggccgtccgg cagggttgcg gtacaagcga gagaaaccgg
     2401 cgcactgccg ttgccgtctc cggtcggggg gcgcaccgca tctggcactg tgacgccggg
     2461 gtcgcaacaa aaacggcagg aaaacgcatt ggccgtaaga ttgcgcgcga tggcccgtgc
     2521 gtgatgcagg tcgaaggcgt gacaggttac ggcgcagtcc ggaaaacgca gggcagccgc
     2581 catggccgcc acgccggtgc ggttgcccgc gaccaacagg tgcgcgggaa caggcgtgag
     2641 gctcccgaga tgatcaagca acagcctttc ggcggcgtgc agtccggaac gggagatgac
     2701 gtgcagcgcg ccgctctgga acgtctgttg gacggtcttg cggccggtga ggtcggtaat
     2761 gcggagggta tcggtcatgc agagggtttc gatttccgtt ggattggggg tgcccgtgtc
     2821 agaagaaggg cccgagctcc agcacggtga tgtcgattgc accgaggcgt tgtgcggcga
     2881 cgcacaggtc atggtcattg ctgaccaaaa acaccacggt gtccggggag gagcttttga
     2941 aaaaacgcag ggtgtcgagc agcacgccgt cggcacggtg ttccccttcg ccacccgaat
     3001 aggtgacacg cacatttgcg gcggcttggt tgtcgctgcg ggtgggtccg tcgaacacga
     3061 gccagacacg gattgaaggc gtattagagg tgagccgcgt cagatcatcg atgagccgct
     3121 gtcgtttctc ggcttccgaa agcgaggccc cgcgcggcgg cgcgtagcgg gagggaaggc
     3181 caaacagcac attgtgtccg tcgatcagca ggatggcggg ttcctctccg gctaaagcgc
     3241 gacccagccg ggctacgggg ccgcggcgtt cagccatgtg gggttccggg ggcggcacgg
     3301 cggtttggag aaccgacagc cgtttccgga aggcctcgcg caggcgatca agggccgcgt
     3361 catccagcac gccgaggggg gcaaagcgtt ccggcaggtc acgcagtccc ggcaggtcgt
     3421 tttccggtgc ggcgtgcagg tgcgcgagaa gggccttttc cagcggggtg gtcgggggtt
     3481 gaggattgag gatctgttca atccgccgca cctccgaggc gagggcggat tccatgcgtt
     3541 ggagttcggg ggtcacccgt aacgcgtggg cgagggtgtc gcgcacctcc tccagaacgg
     3601 accggagctg ttcggcacgc attccgagta cggcgcggtt tccgctgtgc cgatcctgtt
     3661 cccgctgatg ctgcagggcc gcttcggcct gggctacgag gtctgcatgc cggtcggttt
     3721 cacgggcctc cgtctccacc tcgcgcgtgt gcgcgagcca gccgtggaat tcccgggcca
     3781 gtgcgagatc cagagctgcg gcgaggcgtt cgtcacgccg gtcggtttcg cgggcgagtt
     3841 cggctttcgc tgtctccaac tcaacggtgt tccgtcgcag actggcttca gcggcttcgg
     3901 ccttttggcg cgcgagatcc aattccgcct cgctcttttt caggagtgtg gcttggcgcg
     3961 cgaggcggtc ctccacacct tggagacgac gtttttcggt ttggaggcag cgaagccgct
     4021 cttccatgcg cgctttctgg gtcagccagg cttcacgcgt gggaggctgg tttgcggcgg
     4081 gtgcgatacc gatgagatcc gtgagcgggg cgaattgctc gcgcaggcgg tcgagcgcct
     4141 gatccggctt caggggcggc aagggatcgg cctcgcggag ccacgcttcc gcctgatcac
     4201 gcacctcgac gcggtcatcg aggagcatcg cgatccgcag tgcgggatca ccgagcaggg
     4261 acgccaaggc gtggcgcagt tcggcgagga gttcggggga gagcaggttc agcaacgcgg
     4321 cggcgcgcga ccggcgcgcg agaatccggc gcacggattc gggaagcgga cccgcacccg
     4381 tcacgatctg tctcagccgt gcgcggagca cggcgggttt gccggccgga cggaagcctc
     4441 cgacgcggaa tgccggttcg gtggccacga gccgcgacag gtccgcgtcc ggcacctctg
     4501 cgatcaagcg ctgaagctcc tgtccgcatg cgtccggcaa gggtgtttca caaagagaca
     4561 taacggaacc gttatgatag gccgttacgt ccctgcaggc aatccgaatc ccccgg
//
LOCUS       DAOSLE010000184         4545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_16834_length_4545_cov_8.915813, whole genome shotgun sequence.
ACCESSION   DAOSLE010000184 DAOSLE010000000
VERSION     DAOSLE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16834_length_4545_cov_8.915813"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1921
                     /locus_tag="PK576_09780"
     CDS             380..1921
                     /locus_tag="PK576_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61919.1"
                     /translation="MKRIWTSGARKVASLLALTLALTARAGVPVDPNLVALFADTHAT
                     ADAKRTNQRAGVERCVREILALNPRPANLLILGDLSFDHGVTNDYQTLRDLLSPLAKT
                     GVRWHACFGNHDRRASFFAVFPEQRAAHPPVPERHVSRVETPYADFILLDSCLEGPVN
                     GGLDDAQRAWLEKTVAANRKPLFLCAHHGIGETGVAGLMVSNALCKAYLFGHHHNAAH
                     REEKGVRTFCLPSTGHWGDIGFMLMRLTPEEARLTLRQHDYYTRPPTRPEDVNPEWRE
                     RTIRKNGLEWRVPLATAGGFDTLFNGTDFQGWRRPQGDNNHWRILDGVIDYDARSEAK
                     GDKNLWSERSLRDFDLHLEWRIKETPYVNPHARRILPDGSEAAGPDGKPLGLAIPDSD
                     SGVYLRGSGKHQVNIWCWPVGSGEMYGVRRDSAMPPEVRAGVTPRVCADRPVGEWNRF
                     EITVRGNTVRVLLNGQAVLPGVTIPGLPAEGPIALQHHGSLRDGKWVSPPSLVQFRNI
                     FVREL"
     gene            2332..4185
                     /locus_tag="PK576_09785"
     CDS             2332..4185
                     /locus_tag="PK576_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOE61920.1"
                     /translation="MTAERQSMEADIVCVGFGPATAGFLTTLTRAMMKPDGTPAFESK
                     VMPGMPLQVMCYERADDIGFGVSGIVTKGRHISASFPDLDLAKEIPNAAEISREEVAY
                     LFDTVGASRRSAGTKFMDKVFRYGQFAMRKAPSATELPWIPPFLRKEPGLVMSMGSFL
                     SWAGNQLMGSGLVQIWPGTPVSAPIMEGDCVAGVRLTDQGVDKKGNPEAAYMPGMDIR
                     ARLTVVGDGPVGAVGRKLDRHFGLPEGHHQHDWAVGMKAVVELPASCDLKPGTVLHTL
                     GFPEPEIFGFLYVYPDRTASLGIFVPSWMDNPIRTTYRYLQHWMQHPYLWRYLQGGTL
                     RSWGAKSLQESGVRGEPYLVGDGFARIGEGSGTTNVLTNSGVDEAWRSGVLLAEGVID
                     LLARDQPFTKATLTGAYVRRRRQDPLNAELQAAKKARDGFGKGFMLGMMGTGMTGMTG
                     GMLNLSVDSVPPSQRIPTLESVYGDRIAPEELSEAVKQCAKAKQPLHDTIMTRVGWPE
                     IPFDGTLLMSHQDALFKGGKVQAAGGFADHVTFADPALCVSCNERTCIEICSAQALMP
                     GEEGGTPEFDREKCVHCGACIWSCSKALPQDPERANIAFGAGSGGLHSNEN"
BASE COUNT          884 a         1461 c         1343 g          857 t
ORIGIN      
        1 caggaattcc ccctggcgtt ccaaaaaaac ccgagcagtg ccatcccgtg tgttctgtgg
       61 tgaaacaatc gatcccgata gcgatcccga tcccgacccc gatttggatg aaaccatgaa
      121 accagaaacc ttgaaaccaa agagagttga aggttcaact gctctttttg gttaaacatc
      181 gatcccgatt ccgatagcga tagcgatccc gactaataat gcactaacgc actaatgcac
      241 taacgcacta acgcactaat gaactaatga accaacgcca ttcgattccg attccctttc
      301 tgcgcgatct atgtcatgct tttcccggtt gtcgcgcaag ggtgtgcgcg gcattctcat
      361 cagcacaagg ggaaatgaca tgaaacgcat ttggacatcc ggcgcacgga aggttgcgtc
      421 gcttctcgct ctgactctgg ccctcaccgc ccgggcgggc gtgcccgttg acccgaacct
      481 tgtggcgctc ttcgcggaca cgcacgccac ggcggacgcc aagcggacga accagcgtgc
      541 tggtgtggag cgctgcgtcc gcgagattct ggccctcaac ccccggcccg cgaacctgct
      601 gatcctcggt gacctctcgt ttgaccacgg cgtcacgaat gattatcaaa ccctgcgtga
      661 cctgctgagc cccctcgcca agaccggtgt gcgctggcac gcctgcttcg ggaatcatga
      721 ccgccgcgcg tcatttttcg cggtctttcc ggaacagcgc gccgcacacc cccccgtgcc
      781 ggaacgccac gtttcacggg ttgagacccc gtacgcggac tttatcctgc tcgattcctg
      841 tctcgagggt ccggtcaacg gcggtctgga cgacgcccaa cgcgcctggc tcgaaaaaac
      901 cgtggccgca aaccgcaagc cgctcttcct ctgcgcgcac cacggcatcg gtgaaaccgg
      961 cgtggccggt ctcatggttt ccaacgccct ctgcaaagcc tacctcttcg gccaccacca
     1021 caacgccgca caccgcgagg aaaaaggggt ccgcaccttc tgccttcctt ccaccggaca
     1081 ctggggtgac atcggtttca tgctgatgcg tctcaccccg gaggaggcgc gcctcaccct
     1141 ccgccaacac gactactaca cccggccccc aactcggcct gaggacgtca accccgaatg
     1201 gcgcgaacgc acgatccgca agaacggcct cgaatggcgc gtgccgctcg ccaccgccgg
     1261 cggtttcgac acactcttca acggtaccga cttccaaggc tggcgccgcc cccaaggcga
     1321 taacaaccac tggcgtatcc tcgacggcgt gattgattac gatgcccgca gtgaggccaa
     1381 aggcgacaag aatctctgga gcgaaaggtc tctgcgcgat ttcgatttgc acctcgaatg
     1441 gcgcatcaaa gagaccccct acgtcaaccc ccacgcccgc cggatcctgc ccgacggcag
     1501 cgaggccgcg ggtccggacg gcaaaccgtt gggcctcgcc atcccggatt ctgattccgg
     1561 cgtctatctg cgcggctccg gcaagcatca ggtcaacatc tggtgctggc ccgtgggctc
     1621 gggcgagatg tacggtgtgc ggcgcgactc cgccatgccg cccgaagtgc gcgccggggt
     1681 cacgccccgg gtttgcgccg accgcccggt cggcgagtgg aaccgcttcg aaatcaccgt
     1741 gcgcggcaat accgtgcgcg tgctcctcaa cggccaggcc gtgctacccg gcgtgacgat
     1801 acccggtctg cccgctgaag gcccgatcgc cctgcagcac cacggttccc tccgcgacgg
     1861 caagtgggtc agcccgccct ccctcgtcca gttccgaaac atcttcgtcc gcgagctgta
     1921 gacaagcccg cttcggccag aggcatcggg atcggtgtgc ttccccgttc tcgttcgcgt
     1981 gtacgttgac ttgcgaccca cggcattcga cctgcgacgt tttggttgtt tgacgaggca
     2041 gcgggttcgg cggtccggct ccgcgccaat ggcacgtagc tgtggacgtc ccgccgtacg
     2101 ggcacggacc ggaggagcac tgtaaagtgc ggacggacgt ctcggacgcc cgaaggtgtg
     2161 gcaaaatggc taagttatag acaaggcggt cgaacccgac aacgattcag atagcgatat
     2221 cgatcccatg cgtctcgttg acaggaaact gcacgctttg taacatgacg ggcagaaaaa
     2281 acatggggcg agggcgtgac aaaacgacca agctacagac aaggacagat catgacagca
     2341 gagagacaat cgatggaagc cgatatcgtg tgtgtgggtt tcgggccggc caccgccgga
     2401 tttctgacca ctctgacccg cgccatgatg aagcctgacg gcacacccgc ttttgagagc
     2461 aaggtgatgc cgggcatgcc gttgcaggtg atgtgctacg agcgcgccga cgatatcggg
     2521 ttcggtgtct ccggcatcgt caccaaaggc cgccacatca gcgcctcgtt ccccgatctc
     2581 gatctggcga aggagattcc caacgcggca gagatcagcc gcgaggaggt cgcctatctc
     2641 ttcgataccg tgggtgccag ccgccgcagt gcgggcacga agttcatgga caaggtcttc
     2701 cgatacggcc agttcgccat gcgcaaagcg ccttcagcta ccgaactgcc gtggatcccg
     2761 cccttcctgc gcaaagagcc gggtctcgtg atgtcgatgg gctcgtttct gtcatgggcc
     2821 ggcaaccagc tcatgggctc cggccttgtc cagatctggc cgggcacccc cgtgagcgcg
     2881 ccaatcatgg agggcgattg cgtggcgggt gtgcgcctga cggatcaggg tgtggacaag
     2941 aagggtaacc ccgaagccgc ctacatgccg ggcatggaca tccgcgcgcg cctgaccgtg
     3001 gttggggacg gaccggtcgg cgcggtcggc cgcaagctgg atcgccactt cgggctcccc
     3061 gaaggccacc accagcacga ctgggccgtg ggcatgaaag ccgtggtcga gttgcccgcc
     3121 tcgtgcgatc tcaagcccgg cacggttctc cacacgctgg gcttcccgga acccgagatt
     3181 ttcggtttcc tgtacgtcta tcccgaccgc accgcctcac tcggaatttt cgtgccctcg
     3241 tggatggaca atccgatccg caccacatac cggtacctgc agcattggat gcaacaccct
     3301 tacctgtggc gttaccttca gggcggcaca ctgcgctcat ggggcgcaaa gtcgctgcaa
     3361 gagtccggcg tgcgcggtga accctatctg gttggcgacg ggttcgcccg catcggcgag
     3421 ggttccggca cgacgaacgt gctgaccaac tccggtgtgg acgaggcgtg gcgtagcggt
     3481 gtcttgttgg ccgaaggcgt gatcgacctg ctcgcgcgcg accaaccctt caccaaggcc
     3541 acgctgaccg gcgcgtacgt ccgccgccgc cgtcaggatc cgctcaatgc cgaattgcag
     3601 gccgccaaaa aggcgcgcga cggcttcggc aagggcttca tgctcgggat gatgggtacc
     3661 ggcatgaccg gcatgaccgg tggcatgctc aacctttcgg tcgattccgt ccccccctcg
     3721 caacgcatcc ccacgctgga gagtgtctat ggcgaccgga tcgcgcctga agaactcagc
     3781 gaagcggtca aacagtgcgc caaagccaag caacccctgc acgacacgat catgacacgg
     3841 gtcggctggc ccgagatccc ctttgacggc acgttgctga tgtcgcatca ggacgcgctc
     3901 ttcaagggtg gaaaagtgca ggctgcaggc gggttcgccg atcatgtgac ctttgccgac
     3961 cccgcgcttt gcgtttcctg caatgagcgc acctgcatcg agatctgttc ggctcaggcc
     4021 ctgatgcccg gcgaggaagg cggcacaccc gagttcgacc gcgagaagtg cgtccactgc
     4081 ggcgcctgca tctggagttg ctccaaagct ctgccccaag accccgagcg tgccaacatc
     4141 gcgttcggcg ccggctccgg aggactgcat tcgaacgaga actgaccgta gcgcggagac
     4201 acccgccgga aaagaaatcc gcttgcgtac cttttggatc agacggatga ccggaagagg
     4261 gatgttgaat ctaaaaagaa cagttgaccc ttcaactctc tttggtttca gggtttctgg
     4321 tttctggttt caagggttca gggtttcagg gtttcatcca aatcggggtc ggggtcggga
     4381 tcgggatcgc tatcggaatc gattgtttca ccacagaaca cacgggatgg catcgctcgg
     4441 gtttttttgg aacgccagag ggaattcccg attttcatga agttttatgt gtttcatggt
     4501 ttcatccaaa tcggggtcgg ggtcgggatc gctatcggaa tcgat
//
LOCUS       DAOSLE010000185         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17273_length_4454_cov_7.516936, whole genome shotgun sequence.
ACCESSION   DAOSLE010000185 DAOSLE010000000
VERSION     DAOSLE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17273_length_4454_cov_7.516936"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>3023)
                     /locus_tag="PK576_09790"
     CDS             complement(32..>3023)
                     /locus_tag="PK576_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HOE61921.1"
                     /translation="GGDLLVGDAGGNAVFTLDDAATLVVKGAALTVAGNSGTASRGRL
                     ILTGGTLEANAVRGGVGALCRGGTGGAELLADGTTLRAGGATTGLFLGGFDQAELGAQ
                     GLTLDSAGTDLGMGQPFSDLPGATGLFVKTGAGTLSVSNSLHGRTAIARGVLRASEPA
                     AFFGREIAVSNIASLSLEGEAVTLTAGDLTLGSPGGRAILALDAGDSVSVTNALTLHG
                     CAIYFGGAETAGTHTLIRSAGTIDPSCLDHVELLNPAAGKAYAFAIGTDGGGETIQLI
                     VSAYTIADAEWDGSESADWNTADNWTPAGVPTYGTRAKFGAAAARKAVTLSAPGLTTF
                     MEFDSDAGYTLGGTQPLTLPAGMIDNKLGEHEIALPLTLSGEVALPTAAAATTTVSGP
                     VSANLAARVAKSGSGMVVLSGDNAGFQGAWRTGGGRMVFATAGAWGAANSAADAVTVG
                     AGTLTYSGTPAEVSKGIMVDAGGSTLSAILEVKSDLTVNGTVTNHSGILIKRGVGTLT
                     LNVGAGHGNLSSGSGGSASNNDPSGIITFPENGDSPATANGLGGFNLLEGTLRIRGVN
                     STLSIVNQRHFGVLGGAVSSCLADPTLELDAVRLNQGGSGQHLIVGNQIQAGSLARSP
                     TLRLVNGAHLAMDHLRIGRNSAAMVTPTLIMSNATVEASWQVSFGADSTVTPYVRIME
                     GSQIIAKGGAPWGGGICFSRNVDMVVSGNSVVSQTHSDGDMRFNEGGADGTVRWEDGA
                     VMRFAKFSGRNHNTADGLDMIFDNATMEILANGYSYSTRADKQSFIIEDGGLTVKVGS
                     GIRHELTFPLTGAGALTKTGAGELVFAAGLNYTPSATNLTGLATGDYTGGTLVQEGAL
                     SVSNGTIRTDAAVRVAAGARLNLSNSGVALGEVSGSGLITNGTLTAGYRCRVTPAGAD
                     RLTLSDVTLPSGFMVTFDAEAGSELVSGQKVAIADLAGATVPNLDKWSARHVGEKLSA
                     RFTLEGGTVYANVFYSAGTLFQLR"
     assembly_gap    3024..3123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3124..4053)
                     /locus_tag="PK576_09795"
     CDS             complement(<3124..4053)
                     /locus_tag="PK576_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61922.1"
                     /translation="MRLHHPVLLAMVTVCAAVLRTGAFETTNWLSSVAEGYYNDPANW
                     TGGFLPTNGNTGQFTGNQDYVVRFPQGGLVENSVTKVSGLSSGRSVTFDTRGTWWLKA
                     GCDAWPNDWTAFQLGANSSHFFNIEGVLKAAANDYPVMEMSNALFRVQYHSTGMTNVL
                     EEGLLNLYNPGGIHRSAHSLITGSTAPANSLILFKTNSMLRANNVRLRGNRNGHLMRF
                     EGGTHEIYNGMTIAEGGANAGTTCTVEVAGGTLAVRSGSVYVDNKVGTHGNLVIEGSG
                     DMTVRDLVYVAYNATNCSGTVILRDAGRLAVGGD"
     gene            4274..>4454
                     /locus_tag="PK576_09800"
     CDS             4274..>4454
                     /locus_tag="PK576_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005508490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE61923.1"
                     /translation="MRILVTGGAGFIGSHTSVELLQAGHEIVIVDNLCNSSAESVRRV
                     AEITGARPIFRKVDLL"
BASE COUNT          771 a         1535 c         1267 g          781 t
ORIGIN      
        1 agcattcgtt ttcattggaa aacaaagcta tctatcggag ctggaacaag gttcccgcgc
       61 tgtaaaagac gttggcgtaa accgtgccgc cctccagcgt gaaccgggcc gagagcttct
      121 cgccgacatg gcgcgcgctc cacttgtcga gattcggcac cgtggcgccg gcgagatccg
      181 caatcgccac cttctgcccg ctgacgagtt ccgaccccgc ctccgcgtca aaggtcacca
      241 tgaatcccga cggcagcgtc acgtccgaca gcgtcaggcg gtcggcgccc gcgggcgtca
      301 cgcggcagcg gtaccccgct gtcagcgtcc cgttcgtgat caggcccgac cccgagacct
      361 caccgagggc gaccccgctg ttgctgaggt tcagccgcgc acccgccgca acccgcaccg
      421 ccgcgtccgt gcggatcgtg ccgttgctca ccgagagcgc gccctcctgc accagggttc
      481 cgcccgtgta gtcgccggtc gcaagaccgg tgaggttggt cgcgctcggc gtgtagttca
      541 gccccgccgc gaagaccagc tcgcccgcgc ccgttttggt aagcgcgccc gcgccggtaa
      601 gcgggaaggt caactcatgc cggatgccgc tgccgacctt caccgtcaat cccccatcct
      661 caatgatgaa gctctgcttg tcggcgcgcg tggaatacga gtagccattc gctagaatct
      721 ccatcgttgc gttgtcaaaa atcatatcca atccgtccgc cgtgttgtgg ttgcgcccac
      781 tgaatttcgc aaagcgcatc accgcaccgt cctcccagcg gacggttccg tcggctccac
      841 cttcgttgaa ccgcatgtca ccatcggaat gggtttgcga caccacgctg ttcccgctga
      901 ccaccatatc gacgtttcga gagaagcaga tgccgccgcc ccatggagcc ccgcctttcg
      961 cgattatctg gctaccctcc atgatgcgca catacggagt cacagtactg tcagcgccga
     1021 aggacacctg ccaggaggct tcgaccgtcg cattgctcat aatcagcgtc ggcgtgacca
     1081 tggccgccga attccgccct atccgaaggt ggtccatcgc gagatgagcg ccgttcacca
     1141 gccgcagggt cggtgaacgc gccagactgc ccgcctgaat ctggttgccg acgatgaggt
     1201 gctgcccgct gccgccctga ttgagacgca cggcatcgag ttcaagcgtc ggatcggcca
     1261 gacaggagct gaccgcaccg ccaagaactc cgaagtgccg ctgattcacg atcgaaagcg
     1321 tgctgttgac gccccggatc cgcaaggtcc cctccaacag gttgaacccg cccaacccgt
     1381 tggctgtggc cggagagtcc ccgttctccg ggaacgtgat gatgcccgaa ggatcattat
     1441 tactggccga accgcctgat cccgacgaaa ggttcccgtg cccggccccg acattcaggg
     1501 tcagcgtgcc gaccccgcgc ttgatcagga tgccggaatg gttggtgacg gttccgttga
     1561 ccgtcagatc ggacttcact tccagaatcg ccgagagggt gctgccgccc gcatccacca
     1621 tgatcccctt gctgacttcc gccggcgttc cgctataggt caaggttccc gcgccgaccg
     1681 tgaccgcgtc cgcagcacta ttggccgcgc cccaggcgcc tgccgtggca aagaccatcc
     1741 gtccgccgcc ggtccgccac gcgccctgga acccggcatt gtcgcccgac aaaaccacca
     1801 tgccggaacc gctcttggcg acgcgcgccg ccagattcgc cgagaccgga ccgctcaccg
     1861 tggtggtggc cgccgccgcg gtcggcagcg cgacctcgcc cgagagagtc agcggcaagg
     1921 cgatctcatg ttcgcccagc ttgttgtcga tcatgccggc cggcagtgtg agcggctgcg
     1981 tgccgccaag cgtgtagccc gcatccgaat cgaactccat aaaggtcgtc agccccggcg
     2041 cgctgagcgt gaccgccttc cgcgccgcag ccgcgccgaa cttggcgcgc gtgccgtagg
     2101 tcggcacccc ggccggggtc cagttgtccg ccgtgttcca gtccgcgctc tccgagccgt
     2161 cccactccgc gtccgcaatc gtatacgcgc tgacgatcag ctggatggtc tccccgccgc
     2221 cgtccgtgcc gatggcaaag gcatatgcct tgcccgccgc cggattgagg agttccacat
     2281 ggtccaggca cgacggatcg atggttcccg cgctacggat cagcgtgtgc gtgcccgccg
     2341 tctccgcacc cccgaaatag atcgcacagc cgtgcagcgt caacgcattg gtcacgctca
     2401 cactgtctcc ggcatccagg gccaggatcg cccggccccc gggcgagccg agcgtgagat
     2461 ccccggccgt cagcgtcacg gcctcacctt ccagcgaaag cgaggcgatg ttcgaaaccg
     2521 cgatctcacg gccgaaaaac gccgcgggtt cgctggcgcg cagaacgccc cgcgcgatcg
     2581 cggtccggcc gtggaggctg ttcgagacgg agagggttcc cgcgcccgtt ttcacgaaca
     2641 gccccgttgc cccgggaagg tccgagaacg gctgccccat cccgaggtcc gtaccggccg
     2701 aatccagagt gagtccctgc gctcccagtt ccgcctgatc gaaccctccg aggaacagtc
     2761 cggtcgtggc gccgcccgcc cgcagggtcg tcccgtccgc cagcagttcg gctccgcccg
     2821 ttccgccccg gcacagcgcg ccgaccccgc cgcgcaccgc gttggcctcc agcgtgccgc
     2881 ccgtcaggat cagccgtccc cggcttgcgg tcccgctgtt cccggccacc gtaagggccg
     2941 cgcctttcac gacaagcgtt gcggcatcgt ccagcgtgaa cacggcgttg ccgccggcat
     3001 cgccgaccag aagatccccg cccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnatccccg cccacggcaa gccgcccggc atcccgcaag atcaccgtgc cgctgcagtt
     3181 ggtcgcgtta tacgccacat agaccaggtc ccgcaccgtc atgtcgccgg aaccctcgat
     3241 gacgagattc ccgtgggtcc cgactttatt atcgacatac accgagccgc tgcgcaccgc
     3301 aagggtgccg cccgcgacct ctaccgtaca cgtcgttccc gcattcgcgc cgccctcggc
     3361 gatggtcatg ccgttgtaga tctcgtgcgt gccgccctcg aaccgcatga gatggccgtt
     3421 ccggttcccg cgcaaacgga cgttgttggc ccgcagcatt gagttcgtct tgaagaggat
     3481 caggctgttg gccggtgcgg tggaaccggt aatcagcgag tgcgcactac ggtggatccc
     3541 gccggggttg tacagattca gcagcccctc ctccagcacg ttcgtcatgc cggtgctgtg
     3601 atactgcacc cggaagagcg cgttcgacat ctccatgacc gggtaatcgt tggccgctgc
     3661 tttcaacacc ccctcgatat tgaaaaaatg cgaggagttc gcgcccagct gaaaagcggt
     3721 ccaatcgttc ggccaggcgt cgcaaccggc cttgagccac caggtgccgc gtgtgtcgaa
     3781 ggtgaccgag cggccgctcg aaaggccact gaccttggtc acgctgttct cgaccagtcc
     3841 gccctgggga aaccggacca catagtcctg attgccggtg aactgcccgg tgttgccgtt
     3901 ggtgggcagg aagccgcccg tccagttcgc ggggtcattg tagtagcctt ccgcgacaga
     3961 ggagagccag ttggtcgttt caaaggcacc cgtccgcaac accgccgcac acaccgtgac
     4021 catcgccaac aaaacaggat gatgcaacct catccgccac ctcctcaact ccttcaacca
     4081 ttatccattc ggacccgcat tgtaattaaa gatgttccta aacgatgcgg tccattatcc
     4141 aacgacaaat tcagtgtatc gcacgcctcc ttcccgaatc aagaccgaaa acccgatgcg
     4201 cggaaatcaa cttcggattg gttatccgga cgatttcggt taaactggca ctgttctttc
     4261 agaaaggacc ctcatgagaa ttcttgttac cggcggtgcc gggttcatcg gtagccacac
     4321 ttcggtcgag ttgctgcagg ccgggcacga aatcgtgatc gtcgacaacc tttgtaacag
     4381 cagtgcggaa agcgtgcggc gcgttgcgga gatcactggc gcgcggccga ttttccgcaa
     4441 ggttgacctg ctgg
//
LOCUS       DAOSLE010000186         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17402_length_4428_cov_11.498971, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000186 DAOSLE010000000
VERSION     DAOSLE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17402_length_4428_cov_11.498971"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PK576_09805"
     CDS             <1..290
                     /locus_tag="PK576_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61924.1"
                     /translation="WPLRCPFVCVTQFDNMLSHREDNTTRVVLRVMYPLVRKMQEHFL
                     KKVDFFSDRFPRTATDPLFFLHSRAAALQCGLIHPTQMFPFSSHVYETLFS"
     gene            271..681
                     /locus_tag="PK576_09810"
     CDS             271..681
                     /locus_tag="PK576_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61925.1"
                     /translation="MKPSSREILCTACGQTTWARAEPVYDGFKKIGEAFLCTGCGARY
                     ASAAETPFVTASARPRVFTEQDQPVRPVVFREDERRHSCGWCRHFVVNPFAQRCGLTN
                     RHTEATDLCVRFESKPSTETDDTAPDESKDPPRS"
     gene            684..1817
                     /gene="thiH"
                     /locus_tag="PK576_09815"
     CDS             684..1817
                     /gene="thiH"
                     /locus_tag="PK576_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOE61926.1"
                     /translation="MPQHTPFLDLLAEWTPARVQELIASRTPEDVASALAAAGRYQPR
                     HLAALVSPAAEASLEPLARLSAHWTRQRFGHAVQFFAPLYVSNFCCNGCRYCGFNCNT
                     HNTVRRALTLEEAEAEAECLADQGFSHILLVSGEDPVNVPPSYFAELTRRIRHRFASV
                     NIEIYALSLEEYRMLAAAGVDGMTMFQETYHPEVYAACHPSGPKRDFANRLDAFERAA
                     QAGLTFIGLGSLLGINDWREEAFYLGLHAHFLQTRHWRASLAISFPRMRPAHGGTPPA
                     HPVSDTHFVQLLCALRTQLPDVTMTLSTRELPGFRERLVRLAVTKISAGAKTTPGGYT
                     EPTQAEAQFDVADRRPLHEVAAAIAALGFDPVMKDWDRAYGTP"
     gene            complement(1780..2694)
                     /gene="lipA"
                     /locus_tag="PK576_09820"
     CDS             complement(1780..2694)
                     /gene="lipA"
                     /locus_tag="PK576_09820"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOE61927.1"
                     /translation="MQEEPVRSTPARPSWIRVRVGQNERFQETRARLRTHGLHTVCEE
                     AFCPNLGRCWAHGRATILILGDRCTRGCRFCNVEKRSVPPPDPAEPQRVAAAVRETGL
                     KEVVLTSVTRDDLPDGGAAFWAETVEAVHQAVPGILVEVLVPDFAGDAEALDRVIRTR
                     PEVFGHNLETVPRLYPEARPQADYTRSLGVLRQAADAGLIVKTSLMLGLGETAAERLQ
                     VLRDARAAGCRIIYAGQYLQPSPRHLPVVQYVEPAGFDALHEAAIGLGFDYAACAPLV
                     RSSYHEEGQTRFVRDALTASRTRDPSLS"
     gene            complement(2912..4267)
                     /locus_tag="PK576_09825"
     CDS             complement(2912..4267)
                     /locus_tag="PK576_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61928.1"
                     /translation="MAKLQTGITRRAWLGKSGFTAGGFLILPRSVIGASGGVPPSGKL
                     NIGCVGIGGQGGGVAKELATFPEVNLAAFCDVEAKHAERMRKTYPGRPFYSDYRVMLE
                     KEKGLDAVMIGTPDHWHAPIALAAMRRGKHVYVEKPMAHTIEEARRMGEVAAETGVVT
                     QMGNQGHAGEGLRLTKEWLDAGAIGAVREVHVWSDRPGTFWNTQGRRRPAETPPVPPT
                     LDWDLWQGPAPEHPYHPDYVPRRWRGWFDYGCGALGDMMVHNADPAWYALNLGAPDAV
                     EAVTSETNPDSFPQWSIVTWHFAAKGARGPVRVVWYDGGKRPAPPPGLEAERELGDNG
                     LIFLGEKGAMMCPGWSGTPRLVPESAMKTFERPPKTIPRSPGHRKEWVQACIAGRPEE
                     ARAGFHYSAPFTEALLVGVLPIRLGKRIEWDAAAMRAKNAPEADVLIRKPYRKGFGLN
                     G"
BASE COUNT          797 a         1564 c         1259 g          808 t
ORIGIN      
        1 tgtggccatt gcgttgtcct ttcgtttgtg tgactcagtt cgacaacatg ctgtcgcatc
       61 gtgaagacaa tacaacgcgt gttgttttga gagtaatgta tcctttggtt cggaaaatgc
      121 aagaacactt tttaaaaaaa gttgactttt tttctgaccg gtttccccgc acagcaacgg
      181 accctctttt ctttttgcat tcacgcgccg ccgcgctaca atgcggactc attcatccca
      241 cacaaatgtt ccctttttcc agtcacgttt atgaaaccct cttctcgtga aatcctctgc
      301 accgcttgcg gccagacaac atgggcccgc gctgaaccgg tttacgatgg cttcaaaaaa
      361 atcggcgagg cgtttctctg cacgggttgc ggcgcgcgct acgcttccgc agcggagacc
      421 ccctttgtga cggcaagtgc gcgtccgcgc gtcttcacgg aacaggatca accggtgcgg
      481 cctgttgtct tccgtgagga tgagcgccgc cacagctgcg gctggtgccg tcactttgtg
      541 gtgaacccct tcgcgcaacg gtgcggcctg accaaccgtc acaccgaagc cacggatctc
      601 tgcgtgcgtt tcgaatccaa accgtcaacc gaaaccgacg acaccgcgcc ggatgaaagc
      661 aaagacccgc cccggagctg accatgccgc aacacactcc ttttctcgac ctgcttgccg
      721 aatggacgcc tgcccgcgtt caggaactga tcgcctcgcg tacgcctgaa gatgtcgcgt
      781 cggcgctggc cgcggcggga cggtatcagc cgcgccatct ggccgcgttg gtctcgcctg
      841 cggcggaagc ttcgctggaa ccgctggccc gtctctccgc gcactggacg cgccagcgct
      901 tcggacacgc cgtgcaattc ttcgcgccgc tctatgtttc gaacttctgc tgcaacggct
      961 gccgctactg cgggttcaac tgcaacaccc acaacaccgt gcgccgcgct ctgacgctgg
     1021 aggaggccga agccgaagcc gagtgtctgg cggatcaggg gttcagccat atcctgctcg
     1081 tctcgggcga ggatcccgta aacgtgccgc cctcctattt cgcggagctg acgcgtcgga
     1141 tccggcaccg tttcgcctcg gtcaacatcg aaatctacgc gctctccctc gaggagtacc
     1201 ggatgctggc cgcggcgggg gtggacggga tgaccatgtt ccaagagacc tatcatcctg
     1261 aggtgtacgc ggcatgccat ccgagcggac ccaaacgcga cttcgccaac cgcctcgacg
     1321 cattcgagcg cgccgcgcaa gccgggctca cgttcatcgg gctcggatcg ctgctcggca
     1381 tcaacgactg gcgcgaggag gcgttctacc tcggcttgca cgcgcatttc ttgcagacgc
     1441 gtcattggcg cgcctctctg gcgatctcct tcccgcgcat gcgtccggcg catggcggca
     1501 caccgccggc acaccctgtt tcagacacgc atttcgttca gctcctgtgc gcgttgcgca
     1561 cacaactgcc ggatgtgacg atgaccctct caacgcgcga gttgccgggc ttcagggaac
     1621 gcttggtgcg gctggcggtg accaaaatat ccgcaggcgc caaaaccacg cccggcggat
     1681 acacggaacc gacgcaagcc gaagcgcagt ttgacgtggc ggatcgacgt ccgttgcatg
     1741 aggtggctgc cgcgatcgcc gcgctcggct tcgacccggt catgaaagac tgggatcgcg
     1801 cgtacgggac gccgtgagcg cgtcgcgcac aaagcgggtt tgcccttcct cgtgataaga
     1861 gctgcgcacc agcggcgcac aggccgcata gtcaaagccg agtccgatcg cggcttcgtg
     1921 cagtgcgtcg aacccggccg gctccacata ctgcacgacc ggcagatgcc gcgggcttgg
     1981 ttgcagatac tgccctgcgt aaatgatgcg gcatccggcc gcgcgcgcgt cgcgcaacac
     2041 ctgcaaccgc tctgcggcgg tttcgcccaa ccccagcatc agactggttt tgacgatcag
     2101 ccccgcatcc gccgcctgcc gcagcacccc aagggatcgc gtgtagtccg cctgcggccg
     2161 ggcctcggga tagaggcgcg gcaccgtctc cagattatgc ccgaacacct cggggcgcgt
     2221 ccggatcacc ctgtccagcg cctccgcgtc acctgcgaag tccggcacca gcacctcgac
     2281 cagaataccg ggcaccgcct gatgcaccgc ttcgacggtc tctgcccaaa acgccgcgcc
     2341 gccgtccggc agatcatccc gcgtgaccga agtgagcacc acctctttca gaccggtctc
     2401 gcgcaccgcc gcggccacgc gctgcggttc cgccggatcc ggtggcggca ccgaccgttt
     2461 ttccacatta cagaaacggc aaccgcgcgt gcagcgatcg cccagaatca gaatcgtggc
     2521 gcggccgtgc gcccagcaac gcccgaggtt cggacagaac gcctcttcgc acacggtgtg
     2581 caacccgtgt gtccgcaacc gcgcgcgagt ctcttgaaaa cgctcattct gaccaacccg
     2641 cacgcggatc caggatggac gcgccggggt tgagcgtacg ggttcctctt gcatgcgccg
     2701 tatcatatcc tttcgcgtcg cagtttccaa ccccgcaccc gcgtcaggac gcatcgccaa
     2761 agcgggaaat gggtttccgg tttcagtcgg gaacgcgggc gtccccgccc gcgacagcgg
     2821 atccgtcgcc ctgccaaaca acccaaacgc cgcaggccga atgtcgcggg tcgcgggtca
     2881 acgccgcgcg ctgcatgcct tgacgccgcg ctcagccgtt gaggccgaat cccttgcggt
     2941 acggcttgcg gatcagcaca tccgcttccg gcgcgttctt ggcccgcatc gcggcggcgt
     3001 cccactcgat ccgcttcccg agccggatcg gcagcacacc gaccaacagc gcttcggtaa
     3061 agggcgcgga atagtggaac ccggcacgag cttcctccgg acggcccgcg atacaggctt
     3121 ggacccactc cttgcggtga ccgggactgc gcggaatcgt cttcggcgga cgctcaaacg
     3181 ttttcatggc gctctcgggc accaaccgtg gcgtgccaga ccatccggga cacatcatcg
     3241 cgcctttctc gccaaggaag atcaggccgt tgtcacccaa ctcccgctcg gcttccaatc
     3301 cgggcggcgg cgcgggacgc ttgccgccgt cgtaccagac cacccggacc ggcccgcgcg
     3361 cgcctttggc cgcgaaatgc cacgtcacga tgctccactg cgggaaactg tcggggttgg
     3421 tctcggaggt gaccgcctcc accgcatccg gcgcgccgag attcaaagca taccaagcgg
     3481 ggtcggcatt gtgcaccatc atgtcaccca gcgcaccgca accgtaatcg aaccagccgc
     3541 gccagcgccg cggcacgtaa tccggatgat acgggtgttc cggcgcaggc ccctgccaca
     3601 aatcccagtc cagagtcggc ggaaccggcg gggtctcggc gggacgccgc cgtccctgcg
     3661 tgttccagaa agtgccgggc cggtcagacc agacgtggac ctcccgtacc gcaccgatcg
     3721 ccccggcatc cagccactct ttcgtcaacc gcaaaccctc gccagcatgc ccctgattgc
     3781 ccatctgcgt caccacgccc gtctctgcgg caacctcgcc catccggcgc gcctcctcga
     3841 tcgtgtgcgc catgggcttt tccacataca catgcttgcc ccgccgcatc gcggccaggg
     3901 cgatcggcgc atgccagtga tccggcgtgc cgatcatcac cgcatccaac cccttctcct
     3961 tctccagcat cacccggtag tccgagtaga agggacgtcc cggataggtc ttccgcatgc
     4021 gttccgcatg cttggcttcc acatcgcaaa aggccgcgag attgacctcc ggaaacgttg
     4081 ccagctcctt ggcgacgccc cctccctgcc cgccgatgcc gacacatccg atgttcagtt
     4141 tgcccgaagg cggcaccccg cccgacgcgc cgatcacaga tcgcggcagg atcagaaacc
     4201 cgcccgccgt aaatccgctt ttgcccaacc acgcgcgcct tgtgatccct gtctgaagct
     4261 tcgccatctt acacctccgt ttcgcgttca tccgtccgcc acccggacat ccgtatttat
     4321 atatcacaaa acaccggcca ccgtaaactt cgcggaaggg tgacgccgta atcggttcgt
     4381 gacaggagtg tgcgaagaca atacctaaaa agagcagttg aaccttca
//
LOCUS       DAOSLE010000187         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17526_length_4404_cov_7.770752, whole genome shotgun sequence.
ACCESSION   DAOSLE010000187 DAOSLE010000000
VERSION     DAOSLE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17526_length_4404_cov_7.770752"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1421
                     /locus_tag="PK576_09830"
     CDS             222..1421
                     /locus_tag="PK576_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HOE61929.1"
                     /translation="MALPRQNTNIPKIALFLSLNFKAHRDCLSGILDYVRTHKPWHLH
                     LMEGRRGGQGLIQIARWGCNGIIGSALHDDVVRLIRRAQVPSVVLMPPPALRAPSHPL
                     AACSCLTYDSETIGRRAAEYFLKGAYQNFAYVREPLNLFWSKVRGEGFAARLRQAGFA
                     CHVYETGSTQAKHDWAVEQPRLTAWLQALPKPVAVFASMDERGRQVIDACLEAGIRVP
                     EHVAVLGVDNDDLLCEATFPPLSSIETDMRKKGYQVAAHLESLLRNRHTRPRVLKVKP
                     VRVVLRRSSDTTAHAVNPLFSARAFIRETAGQAPIRVPDVARRIGGSRRFAEQRFKAL
                     FKTTIREEIERVRMEKVRELLRDTRIPLREVAKACQFAQAGYLGRRFKLHYGMTPGEY
                     RAGDKRS"
     gene            1469..>2587
                     /locus_tag="PK576_09835"
     CDS             1469..>2587
                     /locus_tag="PK576_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE61930.1"
                     /translation="MERSALPKETAEEIRRRVFEEPGFMRVTQVTRRGGSVFRVSLRP
                     VALQDGRQYQGEMTEAGQTTVKNFTGDAARRGLEEMLGQTGPRELHLVTASGDLHVRV
                     TRKGKAMVSRSAPLTRKAEAYPAHDRIKRQPLTAFDSGALLRVIGIAGADGRIKASMR
                     GKYDQINEFLKVIDTVLDGREERELRIVDCGCGRAYLTLAVHAYLTRVRGYTVRVCGI
                     DRRASVVATARQLAEDLDVAGEVTFLEGDIATCEPEFKPDLLLSLHACDTATDEALAR
                     GIEWTCRYLLCAPCCQHELHKTLKEGGPMQAMMRHGILRERLADLLTDTFRAQILRVL
                     GFRVRVVEFVSAEATARNLLIRAEYCVKPGQAVAVAEYL"
     assembly_gap    2588..2687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2786..4129)
                     /locus_tag="PK576_09840"
     CDS             complement(2786..4129)
                     /locus_tag="PK576_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61931.1"
                     /translation="MKRWTRVIGVWAGCALAVMAGNPTAAYIQRTMKALETSTAEAPA
                     HVRVLFYGQSIVAQGWTKTVQNQLRERYPTVRFEFQNAAIGGYGSQVLIRTADHDLYP
                     WYPDLLFFHVYGPTDKYEEIVRRTRERTSAEIVLWTSHLNSKEKAEELIAKPDERTRK
                     ILEVADTYRCLKIDLRKKWCEHLIAHNLPSTNLLTDSVHLKPEGCDLYARFIAEELVR
                     APELGDNPSASGTITTFGRNAPEVTRGADGSLTLRFTGNRVVALSDGTGAAGARARVL
                     LDGQPMAGKKELWAMTRPSRGPAWMPAINNIGFERPPIKETWTLQALPDSATNANPMH
                     FTLRGSVTGPDGEGWNTNRFVSPSGRVVIEKEDWGCIWQFKYFKKAMPKDFTVTWQAY
                     PLFAETYVPQPAGTRTLLMQGCDNGPHTLTLQPETEGALGISAFVVHAPPRSASQ"
BASE COUNT          790 a         1339 c         1325 g          850 t
ORIGIN      
        1 cacagcctca cagtctcaca gcctttgaga ttgcgggctc cgcccgcatc tcaatacgtc
       61 aaaacccaat gtttacgggg ctttgctaga attccacctg ttcaactgct ccttttagtt
      121 tcaatcgtat acgccttttt tgcgtgtcat tcccatatcc ttttttgcgt aagcggtggc
      181 cggtcttgca ccatatcccc gccttgcgta tactttccca tatggctttg ccccgtcaga
      241 acaccaacat ccccaaaatc gcgctttttc tctcgctcaa cttcaaggcg caccgcgatt
      301 gcctttcggg gattctcgat tatgtgcgca cacacaaacc gtggcacctg catttgatgg
      361 agggacgacg cggcgggcag ggcctgatcc agattgcccg ctggggttgt aacggcatca
      421 tcggcagtgc gctgcacgac gatgtggtgc ggctgatccg cagggcgcag gtcccctcgg
      481 tggtgctgat gcctccgccg gcgctgcgcg ctccgagcca tccgctcgcg gcctgttcct
      541 gcctgaccta cgactccgag accatcggcc gcagggccgc cgaatatttt ctgaagggtg
      601 cgtaccagaa cttcgcctat gtccgcgaac cgctcaacct gttctggtcg aaagtgcggg
      661 gagaggggtt tgccgcccgc ctccggcagg cggggttcgc ctgccacgtt tacgaaaccg
      721 gctcgactca ggccaaacat gattgggcgg tcgagcagcc ccgtctgacc gcctggttgc
      781 aggccctgcc caaacccgtg gcggtcttcg cctccatgga tgagcgcggg cgccaagtga
      841 tcgacgcctg tctggaggcg ggtatccgcg taccggagca tgtggcggta ctgggcgtgg
      901 acaatgacga tttgctttgt gaagcgacct tcccgccgct ctccagcatt gaaacggata
      961 tgcgcaagaa aggatatcag gttgccgcgc atctggagag cctgttgcgg aaccgccaca
     1021 cgcggccccg tgtcctgaag gtcaagcccg tgcgggtggt gctgcggcgt tcgagcgata
     1081 ccacggccca cgccgtcaac ccgcttttca gcgcccgcgc atttatccgg gaaaccgcag
     1141 gtcaggcacc gatccgcgta ccggatgtgg cccggcggat cggcggatcg cgccggttcg
     1201 ccgaacaacg tttcaaagcc cttttcaaaa ccacgatccg cgaggagatc gaacgggtcc
     1261 ggatggaaaa ggtccgggaa ctcctgcgcg acacccggat tccgttgcgt gaggtggcga
     1321 aagcgtgcca attcgcgcag gcgggttatc tcggacgacg tttcaaattg cattacggca
     1381 tgactcccgg cgaataccgg gccggggaca agcgttcgta gcggagaggc atatccggag
     1441 caaaagtcaa accgagcgag gtgaggagat ggaacggagc gcgttgccca aagagacggc
     1501 tgaggaaatc cgcaggcggg tgtttgagga gcccggcttc atgcgcgtga cccaggtgac
     1561 gcggcgcggc gggagcgtgt tccgcgtgag cctgcgaccg gtggcgctcc aagacggacg
     1621 gcaatatcaa ggcgagatga ccgaggcggg gcagaccaca gtgaagaatt ttaccggcga
     1681 tgccgcgcgg cgaggtctcg aagaaatgct gggacagacc ggtccgcgcg agttgcatct
     1741 cgtgaccgcc tcaggggacc tgcatgtgcg ggtcacccgg aaaggcaagg cgatggtgtc
     1801 gcgcagcgca ccgttgacgc gcaaagccga ggcgtatcct gcgcacgacc gcatcaagcg
     1861 tcagccgctg acggccttcg actccggcgc gctcctgcgg gtcatcggca tcgcgggtgc
     1921 ggatgggcgc atcaaagcct ccatgcgcgg caaatacgac cagatcaacg aattcctcaa
     1981 ggtgatcgac accgtgctcg acggccggga ggagcgggaa ctgcgcatcg tggactgcgg
     2041 ttgcggccgg gcctatctga cgctggcagt ccacgcctat ctgacacgcg tccgcggtta
     2101 cacggtacgg gtgtgtggca tcgaccgccg ggccagcgtg gtggccacgg cgcgccaact
     2161 cgccgaggat ctggacgtcg ccggagaggt tacctttctt gaaggtgaca tcgcgacctg
     2221 cgagccggag ttcaaaccgg atctgctgct gagcctgcac gcctgcgaca ccgccacgga
     2281 cgaggctttg gcgcgcggca tcgagtggac gtgccgatac ctgctctgcg cgccgtgctg
     2341 ccagcacgag ttgcacaaaa ccctcaaaga gggcggcccg atgcaggcga tgatgcgtca
     2401 cggaatcctg cgtgaacgac ttgccgacct cctgaccgac acgttccgcg cacagattct
     2461 gcgcgtgctc ggattccgcg tccgcgtggt ggagttcgtc tccgccgagg ccacggcccg
     2521 gaatctcctg atccgggccg agtactgcgt gaaaccgggt caggcggtgg cggtcgccga
     2581 atatctgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gcggtcgccg
     2701 aatatctggc gttgcgggat ttctggaaag tcaccccgtg gctggagacg cgcctgcagc
     2761 gactgatgga aaaacacctc gcccgttact gagacgcgct ccggggcggc gcatgcacca
     2821 cgaacgcgct gatgcccaat gccccctccg tctccggttg cagcgtcaga gtgtgcggcc
     2881 cgttgtcgca gccctgcatc agcagcgtgc gcgtcccggc cggttgcggc acataggtct
     2941 cggcgaacag cggataggcc tgccacgtga ccgtgaaatc cttgggcatc gctttcttga
     3001 aatacttgaa ctgccaaatg cagccccagt cttccttctc gatcacgacc cgtccggatg
     3061 gcgacacgaa acggttggtg ttccatccct cgccatccgg ccccgtcaca ctgccgcgaa
     3121 gcgtgaagtg catcggattc gcattggtcg cggaatccgg cagcgcctga agcgtccacg
     3181 tctccttgat cggggggcgt tcaaaaccga tgttgttgat cgccggcatc cacgccgggc
     3241 ctctgctcgg ccgggtcatg gcccacaact ctttctttcc cgccatcggc tgaccgtcca
     3301 gcaagacgcg ggcgcgggcg ccggccgcgc cggtgccgtc agagagcgcg accacgcggt
     3361 taccggtgaa acgcagggtc aggctgccat ccgcgccccg tgtcacctcg ggcgcgttgc
     3421 ggccgaaagt cgtgatcgtg ccggaggcgg acggattgtc tcccaactcc ggcgcgcgca
     3481 ccaactcctc ggcgatgaac cgggcgtaga gatcgcaccc ttcgggcttc aggtgcaccg
     3541 agtcggtcag caggttggtt gaaggcagat tgtgggcgat caaatgctcg caccatttct
     3601 tacgcagatc gatcttgagg caacggtagg tgtccgccac ctcgagaatc ttacgcgtgc
     3661 gctcatcggg tttggcgatc agttcctccg ccttttcctt tgagttgaga tgactggtcc
     3721 agagcacgat ttccgctgac gtgcgctcgc gtgtgcgccg gacgatctcc tcgtacttgt
     3781 cggtcggccc gtacacgtgg aaaaagagca gatccggata ccaagggtac agatcgtgat
     3841 ccgcagtgcg gatcagcacc tgcgatccat acccgccgat cgcggcgttt tggaactcga
     3901 atcgcacggt gggataacgt tcccgcaatt ggttctgcac ggtcttggtc cacccctgcg
     3961 ccacaatgga ctgaccgtag aacagcaccc gcacatgcgc gggagcctcg gccgtggacg
     4021 tttccagcgc cttcatggtg cgctggatat acgccgccgt ggggttcccc gccataaccg
     4081 cgagcgcaca gcccgcccaa acgccgatca cccttgtcca acgtttcatc aatccccctg
     4141 tttttctctc ttccacattg ttgtgcagag tccgcatcat actcctttga ccggcgcggc
     4201 ggcaagttct aacaacgaga caagccagaa ttaacaatcc aaatcagtat cgctatcggg
     4261 atcgctatcg ggatcgatca tttaaccacg gaacactttc ttgggtttct ggtttctggg
     4321 tttcagggtt tcagggtttc tgggtttctg ggtttcatcc aaatcggtat cggggtcggt
     4381 atcgctatcg gaatcgatca ttta
//
LOCUS       DAOSLE010000188         4386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17618_length_4386_cov_7.360425, whole genome shotgun sequence.
ACCESSION   DAOSLE010000188 DAOSLE010000000
VERSION     DAOSLE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17618_length_4386_cov_7.360425"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PK576_09845"
     CDS             complement(<1..563)
                     /locus_tag="PK576_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61932.1"
                     /translation="MKKLLVIIAVCACVRATLAEPVFWEAASPVTLDATKTFELRVGK
                     APQTGDVSLLVALSAPVDDTFRPTVTLNGVTATKETHAPLATWLPGFDATPIRSPRET
                     REIQAGYRYAFPLSALKAGGNTVALGPAVDTPKVHALALDVDPTSEIVVENDDFRLVV
                     GKDARVKSLVVKKTNEECADAGMQLPL"
     gene            1040..2281
                     /gene="argG"
                     /locus_tag="PK576_09850"
     CDS             1040..2281
                     /gene="argG"
                     /locus_tag="PK576_09850"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOE61933.1"
                     /translation="MALTLTDLRGKTVGACVSGGLDSRTIAKVMVEAGVKVVAFSADL
                     GQPDEKNINDIRKRMAPCGVDTVMVDLKEAMAEACFQVIEAQAMYDGGYWNSTGIARA
                     VTVRGLLPEMRKRGCSVLVHGATGRGNDQMRFERYTNVLAPSMKVYSPWRDAELLKRF
                     PGRTQMVDFLAQFGIQAFVGAKKKYSTDANLAGLSHEAEDLESMETSMLIVEPTMGVW
                     PQQAPDKMEKVAVRFVKGRAVQINGKDVTPLEAMRTANRLAGRNGVGMKHALENRIIG
                     TKSRGVYEAPGMELLGTCLRYVYQATMDRRAGLLFGQLSKLVADQIYDGRYFDPVTQA
                     ARAAIATFARHASGTVEVGLYKGNLFFNSLTGCKASVYNEADSSMEASDGLNPVSSQG
                     FAEIQSVEARMLARAGQIVAK"
     gene            2289..3278
                     /locus_tag="PK576_09855"
     CDS             2289..3278
                     /locus_tag="PK576_09855"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOE61934.1"
                     /translation="MANLKTSYLGLELANPLIVSSNPLTDRADTVADLERAGASAVVM
                     RSIFEEQIRADVSEMVKALAGDTSMAAMEYLRADLPGQLGPEAYLENLAAIRKRVRIP
                     VIASVNCVAADKWVAYARKIEQAGADALELNLYHMPIDPEETGKEVEARRLALVQAVL
                     AEVRLPVAVKLSHHYTSLLPFARRLDALGVKGLVLFNRFLQTDVDIENESIFFTPNYS
                     TPYVLHTQLRWTAVVRDWVRCALAISGGIHSGAELVKALLVGADVGCICSVLHVRKDF
                     GVIREILEDLAAWMDRKGYASLADFRGALRETGRKNGQGFERQQYVETASRVS"
     gene            3301..4065
                     /locus_tag="PK576_09860"
     CDS             3301..4065
                     /locus_tag="PK576_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE61935.1"
                     /translation="MLASGSGGNALYVKGGGVALLVDAGLSSRQLVARMRAADIAPES
                     IHAILLTHDHSDHYKGVDVFSRHYPVRLFANEGTASGVEREVRSLGREWEIFETATSF
                     EVGGLTVEAFSVSHDASDPVGFVLDDGTVRLCVITDLGQATPLVRVKLATCDVAVVES
                     NHDYDMLMQSQRAWTLKTRIAGRSGHLSNDDAADLVRSALSERLRALFLAHISGQCNT
                     PALAFATMADMLKREGRDDVRLEVTSQDQVSTRYVF"
     gene            4088..>4386
                     /locus_tag="PK576_09865"
     CDS             4088..>4386
                     /locus_tag="PK576_09865"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61936.1"
                     /translation="MRIVQIWMSAVMAVSALWGAGVCCGGATVPNTLTEQERREGWRL
                     LWDGRSGDGWRSIRNDNFPTQGWEIADGVLTVLPKNAGGGAGDIITRETFASFIL"
BASE COUNT          792 a         1150 c         1528 g          916 t
ORIGIN      
        1 aaaagcggca actgcatgcc tgcgtctgcg cactcttcgt tcgtcttttt cacgacaagg
       61 gattttacgc gcgcatcttt ccccacgacg aggcggaaat cgtcgttttc cacgacgatc
      121 tcgctagtcg ggtctacatc aagcgcgagc gcgtgaactt tgggcgtgtc gacggcgggg
      181 ccgagcgcga cggtgtttcc gcccgccttc agtgctgaca ggggaaaggc gtaccggtag
      241 ccagcctgga tttcacgggt ttcccgtggg gagcgaatag gcgttgcgtc gaaaccgggt
      301 agccaggtcg ctaacggtgc gtgtgtttct ttggtggccg tcacgccgtt gagcgtcacc
      361 gtcggacgga acgtgtcatc caccggcgca ctgagcgcga ccaagagcga cacgtcgccc
      421 gtctgcggtg ccttgccgac gcgcagctcg aaggtcttgg tcgcgtcgag cgtcaccggc
      481 gacgcggctt cccaaaaaac aggttcggcc aaggtggcac gcacgcaggc gcacactgca
      541 ataatcacca gaagtttttt catttgcttc tccttcggga atcacttttc gggcatggag
      601 ttctccggta tcgcgctttg acttgacacc ggtgacacac ttcgtaccgg ggtcaacctc
      661 cggtgttcat cattgtgcga ttctgggggg ggggaggtca acccccaaac accgctctct
      721 acgctttacc agctttgccc tctccatact cgttcatgtg cctgtgcacg atgactcctc
      781 ttcccgtgtt ttgtaaacgt gtacgtgtgc cgctccgctg tgaacctgtg cggcggtgct
      841 caagaggggc agcggacggt ttcgcgaacc gtccgcggac gtctcggacg cccgagagtg
      901 actggaacgc tgaatcgtag tttgttcgag gaacgggtcc tgtcggctgc ggcgcggctt
      961 ttgagttata ggtgttgatt ttggccggac tactcggtac gcttatgcca tcggaatcga
     1021 tctgacggga gagaacggta tggcactgac attgacggat ttacggggca aaacggtagg
     1081 cgcgtgcgtt tcgggcggct tggacagccg gacgatcgcc aaggtgatgg tcgaggcggg
     1141 ggtgaaggtt gtcgccttct ctgcggatct tggccagccg gacgagaaaa acatcaacga
     1201 catccgcaaa cggatggcgc cttgcggtgt ggatacggtg atggtcgatc tcaaggaagc
     1261 catggctgag gcctgcttcc aagtcattga ggcccaggcg atgtatgacg gcggttattg
     1321 gaattccacc ggcattgcgc gcgcggtgac ggtccgcggg ttgcttcccg agatgcggaa
     1381 gcgcggttgt tccgtgctgg tgcatggcgc gaccgggcgt gggaatgatc agatgcggtt
     1441 tgagcggtac accaacgtgt tggcgccttc gatgaaggtc tactcgccgt ggcgtgacgc
     1501 ggaactgctc aagcgttttc ccggccgcac gcagatggtc gattttcttg cgcaattcgg
     1561 tattcaggct tttgtcggcg cgaaaaagaa atattcgacc gacgccaatc tggcggggct
     1621 ctcccacgag gccgaggatt tggagagcat ggagacttcg atgctgatcg tcgagccgac
     1681 catgggggtg tggccgcagc aggcgccgga caagatggag aaggtggccg tgcggtttgt
     1741 gaagggccgt gcggttcaga tcaacggcaa ggatgtgacg ccgctcgagg cgatgcggac
     1801 ggcgaaccgg ctcgcaggcc ggaacggcgt cggcatgaag cacgcgctgg agaaccgtat
     1861 catcgggacc aagagccgcg gcgtgtacga ggcgccgggt atggagttgt tgggcacctg
     1921 cctgcgctac gtgtatcagg ccacgatgga tcgccgcgcg ggattgctgt tcgggcaact
     1981 ctccaaactg gtggccgacc agatttacga cggaaggtat ttcgatccgg tgacgcaggc
     2041 ggcccgggcg gcaatcgcca cctttgcccg gcacgcttcc ggcacggttg aggtcggcct
     2101 ctacaaaggc aacctgtttt tcaactcgct taccggctgc aaggcttcgg tttacaatga
     2161 ggcggactcc tccatggagg ccagtgacgg gctcaacccc gtcagttcgc aggggtttgc
     2221 ggagatccag agtgtcgagg cccgcatgct ggcgcgtgcc gggcagatcg tggccaagtg
     2281 aggggtccat ggcaaatctg aaaacatctt atctgggact tgagttggcg aatccgctga
     2341 ttgtcagttc aaaccccctt acagaccggg cggatacggt agccgatctg gaacgcgccg
     2401 gtgcgtcggc ggtggtgatg cgctcgattt tcgaggagca gatccgggcg gacgtgtccg
     2461 agatggttaa ggcgctggcg ggggatacca gcatggcggc gatggagtat ctgcgcgccg
     2521 atcttccggg gcagcttggt ccggaggcct atctggaaaa tctggcggca atccgcaagc
     2581 gtgtccggat tccggtgatc gccagcgtca actgcgtggc agcggacaag tgggtcgcgt
     2641 atgcgcgcaa aatcgagcag gccggggccg atgcgctgga actcaacctc tatcatatgc
     2701 cgatcgatcc ggaggagacg ggcaaggagg ttgaggcgcg cagactcgcg ctggtccagg
     2761 cggtgctggc ggaggtccgg ctgccggtgg cggtgaaact cagtcaccac tacacctcgc
     2821 tgctgccttt cgcacgccgg ttggacgcgc tgggggtgaa ggggcttgtg ctcttcaacc
     2881 ggtttttgca gaccgatgtg gatatcgaga acgagtcgat cttctttacg ccgaactatt
     2941 cgacgcccta cgtgttgcac acgcaattgc gctggaccgc cgtggtgcgt gactgggtgc
     3001 gatgcgctct ggcaatcagc ggcgggattc actcgggagc cgaactggtc aaggcgttgc
     3061 tggtcggggc ggatgtgggg tgcatctgtt cggtgctgca cgtgcgcaag gatttcggcg
     3121 tgatccggga aatcctggag gatctcgccg cctggatgga ccgtaaggga tatgcttcgc
     3181 tggctgactt ccggggtgcg ttgcgtgaga ccggtcgcaa gaacgggcag ggctttgaac
     3241 ggcagcagta cgttgagacg gcctcgcggg tttcgtaagg cggatgagat ggaactgtgt
     3301 gtgttagcga gcggaagtgg tggcaacgcc ctttatgtga aggggggtgg ggtcgcgttg
     3361 ctggtggatg cggggctgtc gtcacgccaa ctggtcgcgc gcatgcgggc agcggatatc
     3421 gcccccgaat cgatccatgc gattctgctg acgcatgacc attccgatca ttacaagggg
     3481 gttgacgtgt tcagccgtca ctatccggtg cgcctctttg cgaacgaggg gaccgcttcg
     3541 ggggttgaac gggaggtgcg ctcgctgggc cgggagtggg aaatcttcga gaccgcgacg
     3601 agttttgagg tggggggatt gacggtagaa gcgttttcgg tttcgcatga cgcatcagat
     3661 ccggtggggt tcgtgctgga tgacgggacg gtgcggcttt gcgtgatcac ggatctcggg
     3721 caggcgactc cgctggtgcg tgtcaaactc gcgacatgcg atgtcgcggt ggtcgagagc
     3781 aatcacgact acgatatgct catgcagtcg caacgggcct ggacgctcaa aacgcggatt
     3841 gcgggccgca gcggacactt gtcgaatgac gatgcggcgg acttggttcg gtccgcattg
     3901 tcggagcggt tgcgcgcgtt gtttctcgcg catatcagcg gccagtgcaa tacgccggcc
     3961 ttggctttcg ccaccatggc cgatatgttg aaacgggagg ggcgggatga tgtgcggctg
     4021 gaggtaacca gtcaggatca ggtcagcaca cgttatgttt tttaaacaga caaaggaggg
     4081 tagaggcatg cggatcgttc aaatatggat gagtgcggtg atggcggtgt cggcactatg
     4141 gggtgcgggg gtctgttgtg gcggggccac ggtgccgaac accctgaccg agcaggaacg
     4201 gcgtgaaggg tggcgtttgc tgtgggacgg ccggagcggg gacggctggc gtagcatccg
     4261 gaacgacaac ttccccaccc agggttggga gatcgcggac ggggtcttga ccgtattgcc
     4321 taaaaacgcg ggcgggggcg cgggggacat catcacgcgc gagaccttcg cctcatttat
     4381 cctcaa
//
LOCUS       DAOSLE010000189         4352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17814_length_4352_cov_7.707005, whole genome shotgun sequence.
ACCESSION   DAOSLE010000189 DAOSLE010000000
VERSION     DAOSLE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17814_length_4352_cov_7.707005"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..686)
                     /gene="dcm"
                     /locus_tag="PK576_09870"
                     /pseudo
     CDS             complement(1..686)
                     /gene="dcm"
                     /locus_tag="PK576_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1389..2141)
                     /locus_tag="PK576_09875"
     CDS             complement(1389..2141)
                     /locus_tag="PK576_09875"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE61937.1"
                     /translation="MRNQNRMWLGLVVLVSGCSLTSPEKPDADTCCPKTASCQTCPVR
                     TGEPLHPDSRNWRPLFHLNLANAVFTPGVWFINDKGDLTATKDEAIWTDRDFENFILD
                     LEYSCDPAANSGVLLYCSDMKNWIPNTVEVQILDDNHPHWVNEKPFAKNGGLYGHLPP
                     KVNNVKPAGEWNRMTVTARGKRIKVAVNGEITVDTDLSQWTSAKKNPDGTDIPPWLNR
                     PWAELPTRGRIGLQGKHGNASIRFRFIRIREL"
     gene            complement(2230..3750)
                     /locus_tag="PK576_09880"
     CDS             complement(2230..3750)
                     /locus_tag="PK576_09880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE61938.1"
                     /translation="MKIAIIHQAVPDTAAADERDVLVQAAAVRTALTTRGHTVTVLAC
                     TTDLGNVRRKLELLMPDCVFNLVETLDGSGRLIHLAPAVWEAAGIPFTGSSAEALYLT
                     TQKRLAKERMLAAGLPVPEWIEAGDGRAPHAVPRADWIVKSIWEHASVGLDDNSILRG
                     LTPEEVAAHLPEGSFAERFIDGREFNLALLAGPDGPVALPPAEIVFENFPAGKPRIVG
                     YQAKWDEASFEYAHTVRRFADPSEDGVLLAELKCLASRCWELFRLDGYARVDFRVDGT
                     GRPWILEVNANPCLAPDSGFAAALDRAGIAYADAMERIVRAAVKAERPSGNSCGAPAG
                     NVYRRPCPDSPSVTFRYEVLPADVDTIREITVATGFFHDYEIPVAVELAEERLAKGTA
                     SGYEFVLAECDGKVIGYTSFGPIPCTRSSFDWYWLAVRPEFQGFGFGRRLLEQVEARV
                     RAMGGTRLYCETSGRPQYAATRCFYERMGFELGELLDNYYDVGDGRATYVKRLDQV"
     gene            3730..>4352
                     /locus_tag="PK576_09885"
     CDS             3730..>4352
                     /locus_tag="PK576_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61939.1"
                     /translation="MNDRDFHAFSSTLSKTDRGRHTLCPTASTVPSNRLGIQCRAGAG
                     IAQERNTIFPRPGVRIRPQIALHFEHQDTPAFHEVSVSWFHGFMVSGFQGFKVSSKSG
                     SGSLSGSGSLSGSGSIASPRDTFLGFMVSGFHPNRYRDRDRYRDWDRFTTNSRNGIAR
                     VFLERQGEFLIFMKFHVFHGFWVSGFQGFIQIAIGIGIAIGIGIASPR"
BASE COUNT          942 a         1286 c         1194 g          930 t
ORIGIN      
        1 aacaacagac ggggattgct ttgttttttc atgattaggc acatcatctg taatcggcgg
       61 gacttccagg ctcttcttgc ggaaacatcc acatctctat aaggatccgg agatggtatc
      121 ttaaattgca aattaatgtc tttgcgaatt cctactataa ttatccgttg tcgcgcttga
      181 ggcactccat atttgtcaaa agagtaatag tgcggataaa gcgagtagcc agcattaaac
      241 agtgatttca ttattttaga aaaagccttg ccgtcattgg cggattgtaa tccgccaaca
      301 ttttctgcta agaaccactg cggctggaaa aactccaaag ccttcacgca ataatggtac
      361 aatgggccaa aagcgccatg cacacctttc tgctctccca ctgcactgaa atcattgcag
      421 gggaatccaa aagctagggc gtcaacatca gaaattgatt ttaaacgatt caaatccagt
      481 tttctaatat ctgcgcacac aacactcgac gagtcacctg cgcatacatt gtgcgcaaat
      541 gttttacagg ctgccggatc ataatccgtt gcccatgcat gacgaatgga atactccgaa
      601 ccaaatcgcg aaaccacctt tgcgttcttt gcaccgtaag cgaggccgcc cggaccacaa
      661 aacaattctc ccaagcgaaa agacacttgc cttttcaatt tcgattttgg ttttggaaaa
      721 gatacaatag ttattgtatc cataagagat gttttgtggg aaaacatgct aggatcataa
      781 taaattttta tgtccgcgcc aagggctttt ttacgttccc gtcaggtgct gaatgggact
      841 cattgtgaca tgcgggaggg gtttcggcaa ggctgatccg gcagaggccg ggttccggac
      901 ggcgcatccg ttttcacctc tcccctttca tgtgtgtcag cggacactga acatgaagcc
      961 atacggactg agccagcacc gatgttcttc gttcagcgga acaggacccg cgtcccgcgc
     1021 gcattgatcg caagcgaaat tcccgtcgga ccgaacacga gacggacgga tttacgcgaa
     1081 atgatcgatc cggcaacctg ccaggcattc ggatccatcg ccgtgaccga cgtcgccgat
     1141 acaatcggca ggaccgtccc cggcgggaac gacacatcca gcggcccccc tccttacttc
     1201 gggtggacgg tccaagacgg actccggtta ggaagaacca tccgcgcgtg tggtggcgga
     1261 caccccggaa gatgcgtctc gggtttctgc ttcgtgtatg tgcaccgctg cgctgtgaac
     1321 gcgtacgggg catcaaacac gcaacatgca gattatcggc agagacgcct ccccgaaccg
     1381 tccgcagatt acaactcgcg gatgcggatg aagcggaagc ggatcgaggc gttgccgtgt
     1441 ttgccctgca aaccgatacg gccccgggtc ggcaactctg cccaaggacg gttcagccag
     1501 ggcgggatgt ccgtcccgtc ggggtttttc ttggccgagg tccactgcga aagatcggtg
     1561 tccacggtga tctcgccgtt gaccgcgact ttgatgcgct tccctcgcgc ggtcacggtc
     1621 atgcggttcc actcgcccgc aggcttcacg ttgttgacct tcggcggcag atgaccgtag
     1681 agtccgccgt tcttggcgaa aggcttctca ttgacccaat gcggatggtt atcgtcgagg
     1741 atctgcacct ccaccgtgtt cgggatccaa ttcttcatgt cggaacagta gagcagcacg
     1801 ccgctgttcg cggccggatc gcaggagtat tccagatcaa ggatgaaatt ctcaaagtcg
     1861 cgatccgtcc agatcgcctc atccttcgtg gcggtcagat ctcccttgtc gttaatgaac
     1921 cagacaccgg gggtgaacac ggcattcgcc agattaaggt ggaaaagcgg ccgccagttc
     1981 cgggaatccg gatgcagcgg ctcccccgtg cgcactggac aggtctggca cgacgccgtc
     2041 ttcgggcaac atgtgtccgc atcgggtttt tccggagagg tcagcgaaca ccctgacacc
     2101 agcacgacca accccagcca catcctgttt tggtttctca tcccgactct ccttacgcgg
     2161 ttccgaattg ccgtaaagct tacgaagctt gggtcataat ttcaatccca atccggtcgc
     2221 ggaaaccgtt catacctgat ccagccgctt cacataagtc gcgcggccgt cgccaacgtc
     2281 atagtagttg tcgagcagtt cgcccaactc gaatcccatc cgctcataaa aacagcgggt
     2341 cgcggcatac tgcggacggc ccgaggtttc gcaatagaga cgtgtgcctc ccatcgcgcg
     2401 gacccgcgcc tcgacctgtt cgagcaaccg ccgcccaaat ccgaaaccct gaaactcggg
     2461 acgcaccgcc aaccagtacc agtcgaaact gctccgggta caggggatcg gaccgaaaga
     2521 agtgtatccg atcaccttcc cgtcgcactc ggccagcacg aactcgtatc ccgaggccgt
     2581 gcctttggca aggcgttcct cggccaactc caccgccacg gggatctcat aatcgtgaaa
     2641 gaacccggtc gcgacggtga tctcgcgaat cgtgtccaca tccgcaggca gcacctcgta
     2701 gcggaaggtg acggacggag agtccgggca tggcctgcgg tacacgttcc cggcgggtgc
     2761 gccgcacgaa tttccggacg gacgctccgc cttcaccgca gcccgcacga tccgttccat
     2821 ggcgtcggca taggcgatgc ccgccctgtc caacgcagcg gcgaaaccgc tgtccggcgc
     2881 gaggcagggg ttggcgttga cctcgaggat ccaaggccgc cccgtgccgt ccacgcggaa
     2941 atccacgcgc gcgtacccgt cgaggcggaa aagctcccag caacgcgacg cgaggcattt
     3001 caattcagcg agcaacacgc cgtcctcaga cggatcggcg aaacgccgca ccgtgtgcgc
     3061 gtattcgaac gacgcctcat cccacttggc ctgatagccc acaatgcgcg gtttcccggc
     3121 agggaagttt tcgaacacga tctctgcggg cggcagcgcc acaggaccgt ccggcccggc
     3181 gagcagagca agattgaact cgcggccatc gataaaacgt tcggcaaagc ttccttccgg
     3241 aaggtgtgcc gcaacctcct ccggcgtcaa accgcggaga atgctgttgt cgtcaagccc
     3301 gaccgaagca tgttcccaga tggacttcac aatccaatcg gcccgcggca cggcatgcgg
     3361 ggcgcggccg tctccggctt cgatccactc cgggaccggc aaccctgcgg cgagcatgcg
     3421 ttccttggcg agtcgtttct gagtcgtaag gtagagggct tccgcagaac tgccggtaaa
     3481 cggaatgccc gccgcctccc acacggccgg ggcaaggtgg atcaggcgtc cggaaccgtc
     3541 gagcgtctca accagattga agacacagtc cggcatcagc agttccaact tacgccgcac
     3601 gttgccgagg tccgtggtac aggcaagcac cgtgacggtg tggccgcggg tggtcagggc
     3661 agtgcgcacg gccgctgcct gaaccagcac gtcgcgctcg tcggccgccg cagtgtcggg
     3721 aacagcttga tgaatgatcg cgattttcat gcgttttcca gtaccctttc caaaacggat
     3781 cgcggccgtc acacgttgtg ccccaccgca tcgacggtcc cgtccaatcg gttgggaatc
     3841 cagtgtagag ccggagccgg gattgcgcaa gagcgcaaca cgattttccc gaggccgggc
     3901 gtacgaatcc ggccacaaat cgcattacac tttgaacacc aagatacccc tgcttttcat
     3961 gaagtttccg tttcatggtt tcatggtttc atggtttcag ggtttcaggg tttcaaggtt
     4021 tcatccaaat cgggatcggg atcgctatcg ggatcgggat cgctatcggg atcgggatcg
     4081 attgcttcac cacgggacac tttcttgggt ttcatggttt cagggtttca tccaaatcgc
     4141 tatcgggatc gggatcgcta tcgggattgg gatcgcttca ccacgaactc acggaatggc
     4201 atcgctcggg tttttttgga acgccagggg gagttcctga ttttcatgaa gtttcatgta
     4261 tttcatggtt tctgggtttc tgggtttcaa ggtttcatcc aaatcgctat cgggatcggg
     4321 atcgctatcg ggatcgggat cgcttcacca cg
//
LOCUS       DAOSLE010000190         4322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_17961_length_4322_cov_6.425592, whole genome shotgun sequence.
ACCESSION   DAOSLE010000190 DAOSLE010000000
VERSION     DAOSLE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17961_length_4322_cov_6.425592"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1882
                     /locus_tag="PK576_09890"
     CDS             149..1882
                     /locus_tag="PK576_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOE61940.1"
                     /translation="MKMKNRAFVFGVVGVVAAWGSCREPMTFCNPLDLDYGFRNELGF
                     HEGADPVCLTYKGDYYLFASKSGGYWWSPDFVCWRKVSPPNLPLANYAPTAMEYRGEL
                     YFTASGAGIFKSSRPKDEDSWELVNAQKSNVDPALFADDDGRVFLYAGCAAGKRRGAP
                     TVIELDPTNQFKRIGAAAECVRPLESERGWEVRGNTNQGHGLDRSDKEPWIEGSWMNK
                     HGGKYYYQYAAPGTEFDTYGDGVCVSRSPMGPFVYEPYSPFSFKPTGFIPGAGHGATF
                     QDKHQRLWHVATGVIRGVERRVVLFPARIDAAGRLFAETAFGDLPQYLPGKNPSPGKG
                     NLVGWMELGCGKAVSASSEHSGARLARCAFDTSIRSWWQPKGSDSNAWIGVDLGAIKR
                     VCAIQVHFAETEEVRRSTNSLPQDFVQKYRVDCSDDGVHWVTRVDQSQNPKDRMNAYH
                     ELENPVATRHLRLINCGTKSGYFAVAGLRVFGTGEGTPPAPASNVRVIRGKADRRHVT
                     VEWEGGAGAKGAVVRYGVAADAMFTPFEIRGQTRLDIYSLNAGAPYWFTVDTFNENGI
                     TRMRADPVFVE"
     gene            1952..4210
                     /locus_tag="PK576_09895"
     CDS             1952..4210
                     /locus_tag="PK576_09895"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61941.1"
                     /translation="MLFVALGLLPLAVSAATGGDPLKGLVCGTGDACPQGYVPPLLGN
                     GELSMTVDWTCGMRERSYAGIRPAIYWAGRRLPPPDGGLFEFGKFNHRVIIGGVLRDY
                     PDVWEQELDVRRATLFSRNSYTNGLRIESEIIVPLTRNLIAFRRTLVSTATAPLEVTL
                     DYIFQAPKHPRLTGTWAWRKETRYAVYSCRTYGNDIRDAMIAAYKGADEPAHYTVKND
                     RDATLSATFTLQPGERKVTTFFIQYEDTLDSGKLADRIQNTRQWVWDNGFNALLREHE
                     QAWAAYHGESSIEVPEPKIQRMIDVAQYHLRVNATRWSFPVGIFPGHWHGKYFGWDEM
                     FCHQGLLLANHVAVARRCPEFRKALLKTACARVSHYSRKGKYGARYLWESYEDGRVEG
                     APQGFWLDHIFHMSNIAKSVWTQYLYSGDLEYLKEVGYPVLIECARYYRSHWVYEDSD
                     GTWYIGKCTDLERLGPARERPFMTTCGAIYTLRAAADAAERLHMNLDEAADFRTTADK
                     LVASLPHRDGAYIAYKNCTVPSVATLGGLFPYPIFDKSNDLQREAAYRFIRDGRLAGN
                     MYPVGKQICPWYAGKMAVAMTLLEDNVEPVKLLREAAGVTGLFGETFEINEPGTVVAH
                     PWFSTASGNVLVALCHMLLHNAGDELRLGIGVPDEWKNYAFRLPAHGGLLADACVKDG
                     RLATLRLTPNATVKKPQSFTLVFREGMLPAAGLSASCVKTQTREDGKVYVALAVNGPV
                     TLLEAGKNQKGL"
BASE COUNT          862 a         1176 c         1390 g          894 t
ORIGIN      
        1 gattttggcg ttggcgtcaa tatcgttcgt aagattaagg gtgatccgga ctatccgacg
       61 atcaaggaat ccattggacg aactgacccg gtacgttgta tcctgtcttc atcggcatcg
      121 agggcgcagc cccggggcat tttttaatat gaagatgaag aacagggctt ttgtgtttgg
      181 ggtggtcggc gtggtcgcgg catggggatc ttgccgggag cccatgacgt tctgcaaccc
      241 gcttgatctc gattacggct tccgaaacga gctcgggttt cacgaaggcg cggatcccgt
      301 ctgtctgacg tataagggag attattacct gttcgcctca aaatcgggtg gctactggtg
      361 gtcgcctgat tttgtctgct ggcggaaagt gtcgccgccc aacctgcctc tggcgaacta
      421 tgcgcccacg gccatggaat atcgggggga gctttatttc acggcttcgg gtgcgggtat
      481 cttcaagagt tcgcggccca aggatgagga ctcatgggag ttagtcaacg cgcaaaaaag
      541 caatgtggac cccgcccttt ttgccgatga cgacggtcgc gtgtttcttt acgccggttg
      601 cgccgccgga aaaagacgag gggcgcctac cgtcatcgag cttgatccga ccaaccagtt
      661 caagcgtatc ggtgccgcgg cggaatgcgt tcggccgctt gagagcgaac gcggttggga
      721 ggtgcggggc aacaccaatc agggacatgg tctcgaccgc tccgacaagg agccgtggat
      781 cgaggggtcc tggatgaata aacacggggg gaagtattac taccaatacg cggcacccgg
      841 taccgagttt gacacgtatg gtgatggcgt ttgcgtttcc cgttcgccga tgggaccgtt
      901 cgtgtatgaa ccgtacagtc ctttctcgtt caagccgacg gggtttattc ccggtgccgg
      961 ccacggggcg acctttcaag acaagcacca acgcctttgg catgtcgcca ccggtgtgat
     1021 tcgcggtgtc gagcgtcgtg tggtcctttt tcccgcccgc atcgatgcgg caggacggtt
     1081 gtttgcggaa acggcctttg gtgacctgcc gcagtatctg cccggcaaaa atcccagccc
     1141 cgggaaaggg aatcttgtcg gctggatgga actgggttgc gggaaggccg tcagcgcttc
     1201 gtcggaacat agcggcgccc gtctcgcccg atgcgccttc gacacgagta tccgatcctg
     1261 gtggcagccc aagggatcgg actcgaacgc ttggatcggt gtcgatctcg gcgcgattaa
     1321 acgggtgtgc gcgatacagg ttcatttcgc ggagacggaa gaggtgcgga ggagcacaaa
     1381 cagtttgccg caggattttg ttcaaaaata tcgggtggac tgctccgacg acggcgtgca
     1441 ctgggtaacc agggtcgacc aatcccaaaa cccgaaggac cggatgaacg cttatcacga
     1501 gctggaaaat cctgtggcca cgcggcacct tcggcttata aattgcggta cgaagtcagg
     1561 atactttgct gttgcgggtc ttcgcgtttt cggaaccgga gagggaacgc ctcccgcgcc
     1621 ggcaagcaac gttcgggtga ttcgcgggaa ggccgaccgg cggcatgtga cggtggaatg
     1681 ggagggcggg gcaggcgcca aaggcgcggt cgtacgttac ggtgtggcag cggatgcgat
     1741 gttcacgccc tttgaaatcc gcggtcaaac gcggctcgac atttacagcc tgaatgcggg
     1801 ggcgccctat tggttcacgg tggatacgtt taatgagaac gggatcaccc gcatgcgtgc
     1861 ggaccctgtt tttgttgagt gagggaagag ggggggggac aggagacaaa agacgaaggc
     1921 attagtcatg ggaacgcaaa ggatgcggca tatgttgttt gtggcgctgg gcctcctgcc
     1981 tttggcggtc tcggccgcga cagggggcga tccgttaaag gggctggtct gtggcacggg
     2041 ggatgcttgt ccgcagggct acgtgccccc gctgttgggt aacggtgagt tgagcatgac
     2101 cgttgactgg acgtgcggta tgcgcgagcg cagctatgcc gggatcagac cggccatata
     2161 ctgggctggc cggagattgc cgccccccga tggcggattg ttcgagttcg gcaagttcaa
     2221 ccaccgcgtt atcatcggcg gggtattgcg tgactatccg gatgtctggg aacaggagct
     2281 ggatgtccgg cgcgcgacac tgttcagccg caacagttac acgaacggcc tccgcatcga
     2341 gagcgagatc atcgtgccgc ttacccgtaa cctgatcgcc ttccgccgca cattggtctc
     2401 cacggcaacc gcgccgctgg aggtcacgtt ggactacatc tttcaggcgc ccaaacatcc
     2461 gcgcctgacc ggaacgtggg cgtggcgtaa agaaacgcgt tacgccgtct attcctgccg
     2521 gacttacggg aatgacatcc gtgatgcgat gatcgcggcc tataaaggag cggacgaacc
     2581 ggcacactac acggtcaaga acgaccggga cgcaacgctt tccgccacat ttacccttca
     2641 gcccggtgag cggaaagtca ccacgttctt cattcagtat gaagacacgc tagattccgg
     2701 caagcttgcc gaccgcatcc aaaacacacg ccagtgggtt tgggacaacg ggttcaacgc
     2761 cctgttgcgc gagcatgagc aggcttgggc cgcatatcac ggcgaatcgt ccattgaggt
     2821 accggagccg aaaatccagc gtatgatcga tgtcgcgcag taccacctgc gcgtcaatgc
     2881 cacgcgctgg tctttccccg tggggatctt cccggggcat tggcatggga aatacttcgg
     2941 atgggacgag atgttctgcc atcaggggtt gttgctcgcc aatcacgtcg ccgtggcccg
     3001 ccggtgtccg gagttccgta aagccctgtt gaaaaccgca tgcgcccgtg tgagccatta
     3061 cagcagaaaa ggcaagtatg gcgcccgcta tctatgggaa tcgtatgagg acgggcgcgt
     3121 ggagggagcg ccgcagggct tctggctgga ccacatcttc cacatgtcca atatcgcgaa
     3181 aagcgtttgg acgcagtacc tttacagtgg ggatttagaa tacctcaagg aagtcgggta
     3241 tccggttctg atcgagtgtg cccgctatta ccgctcgcac tgggtgtatg aggattccga
     3301 cggcacgtgg tacatcggca agtgcactga tctggagcgc cttggcccgg cgcgcgagcg
     3361 accgttcatg accacctgcg gcgcgatcta caccttgcgg gccgcagcgg atgcggctga
     3421 acggcttcac atgaatctgg atgaggcggc ggatttcaga acaacggctg acaagttggt
     3481 tgcctcgctg ccgcatcgcg acggagctta catcgcttac aaaaactgca cggttcccag
     3541 cgtggccacc ttgggagggc tcttcccgta tccgatcttt gacaaaagca acgacttgca
     3601 gcgtgaagcg gcctaccgct tcattcggga cggccgcctc gctggcaaca tgtatccggt
     3661 cggcaaacaa atctgtcctt ggtatgcggg caagatggcg gttgccatga ccctgttgga
     3721 agacaacgtg gagccggtca aactgctgcg cgaggccgct ggcgtgacgg gattgttcgg
     3781 cgagaccttt gagatcaacg agcccggcac ggttgtcgcg catccctggt tctcgaccgc
     3841 ctcgggcaat gtgcttgtcg ccctgtgcca catgctcctg cataacgccg gggatgagtt
     3901 gcggcttggc atcggtgtgc cggacgagtg gaagaattac gccttccggc tgccggcgca
     3961 tggaggcctc cttgccgacg cctgtgtcaa ggacggccga ctcgcgacgt tgcgcctgac
     4021 accgaacgcg acggtcaaga aaccccagtc cttcacgctc gtcttccggg aggggatgct
     4081 gccggcggcg gggttatccg cctcgtgcgt gaagacgcag acacgggaag acggcaaggt
     4141 ctatgtggcc cttgcggtga acggccccgt cacgcttctt gaagccggaa aaaaccagaa
     4201 ggggttataa cgaaaagaac gggttggctg aaagcgttcc gctccttgtt gatgatctgt
     4261 gccgagccgt attgagatgc gggcggagcc cgcaatctca aaggctgtga ggctgtgagg
     4321 ct
//
LOCUS       DAOSLE010000191         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_18028_length_4310_cov_8.837133, whole genome shotgun sequence.
ACCESSION   DAOSLE010000191 DAOSLE010000000
VERSION     DAOSLE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18028_length_4310_cov_8.837133"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK576_09900"
     CDS             <1..275
                     /locus_tag="PK576_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61942.1"
                     /translation="KDMKLHEKREFPPAFQKKPSDAIPCVRGEIIDSDTDSDSDWNGT
                     MKPKRIDPDPDPDSDLDETLKPETLKPRNLKPRNPETKERCFQRSF"
     gene            complement(319..963)
                     /locus_tag="PK576_09905"
     CDS             complement(319..963)
                     /locus_tag="PK576_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61943.1"
                     /translation="MAALKLDMGAEMGRGWRLFRANMGMLVLAGLICTVVGALTCGIL
                     AGPLFAGLLLVLDRLIREDPVRPQAGDVFKGLDFFMQALVLSLICFVISFVLSLVPVL
                     GHLAGLLVGSVLMWSMMRIVYRRETAVEALKAVGGDIGRGDFTMPFLFGALVSLIGGL
                     GFVACGVGIFFTLPLSYCMLVCSYHTLYESEGGVTTEAEMLSAEPPPPPDDLRL"
     gene            complement(1106..2479)
                     /locus_tag="PK576_09910"
     CDS             complement(1106..2479)
                     /locus_tag="PK576_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE61944.1"
                     /translation="MRHAFGFSRRRFLASSAALATFTIPRRSLFGQTLNAPPGKQFRF
                     AKIGCGGMGGADLNSTVSSGGIVVGLCDVDPKRAEKAYQSYPDVPKFTDYRKMLDKLE
                     KEIDGVVISTPDHTHACAALDAMRRGKHVYVQKPLARTYEECRLLLEATRKYKVVTQM
                     GNQGHAGPGLILWKKMMDAKAFGEIEQVHTWSDRPIWPQGMTEKPKEDPVPEGFDWDS
                     WIGPASMRPFSTAYVPFKWRGWWDFGCGAIGDMACHNMDPAFWILQLGMPAAIRAEAS
                     SPAGIAYPAWSIIEFTFPATPVCPKGVKVTWYDGKKLPTLPANAHPELKPGTNGCLII
                     GSKMSVMGGSHAAPPMPIALGGQAYGPAVKDAERHWREELKQTKGCSHYGQWVEACKE
                     NDMTRPGSQFEYSVPMTQTLLLGCIALRYPGVELKWDSARNRFSNHEEANRWLADAPR
                     AGFDLKV"
     gene            complement(2565..3134)
                     /locus_tag="PK576_09915"
     CDS             complement(2565..3134)
                     /locus_tag="PK576_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61945.1"
                     /translation="MKIPLSALFASACVLFGTGCADSASTRNPSGERMKNWTEEFDRL
                     LPLLGHRNWILVTDQAYPYQSAPGITYFDTRAPLPEVLARVATSLRNSAHVKPIFYTD
                     LELGALTEELVPGIDAFRAATRAVLPADGVQTLPHESVFAKLDAASKLFHVVVLKTDC
                     VIPYSSVFIELDCAYWDADREQALRARLR"
     gene            complement(3131..3739)
                     /locus_tag="PK576_09920"
     CDS             complement(3131..3739)
                     /locus_tag="PK576_09920"
                     /inference="COORDINATES: protein motif:HMM:NF018627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOE61946.1"
                     /translation="MRIYIDFDDVLCETARPLAALAGELFQSTVPYEAITGFDLQQAF
                     GLTDAEIALLMERAHQTAFLEALPPAPGGLQGVRELETRGHDLMIVTGRPASSHSGSC
                     GWLRKHGLAHLELLYVDKYGRASGQPTPDTPRTLSREEFADMSFDVAIDDSPAALDLL
                     APRRDCTVIVYDRPWNRHYAHRPNMRRTGSWREIVSLIGDLA"
     gene            3964..>4310
                     /locus_tag="PK576_09925"
     CDS             3964..>4310
                     /locus_tag="PK576_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOE61947.1"
                     /translation="MATLIDPSTLKITDVRFADIDGAPKRCTLIKVYTNQGLIGYGEV
                     RDASSRTYAAMLKSRILGENPCNVEKIFRRIKQFGGHSRQAGGVCGIEVALWDLAGKA
                     WGVPVWQLLGGKFR"
BASE COUNT          852 a         1338 c         1383 g          737 t
ORIGIN      
        1 tgaaagacat gaaacttcat gaaaagcggg aattcccccc ggcgttccaa aaaaaaccga
       61 gcgatgccat cccgtgtgtt cgtggtgaaa taatcgatag cgataccgat agcgatagcg
      121 attggaatgg aaccatgaaa ccaaagagaa tcgatcccga tcccgatccc gatagcgatt
      181 tagatgaaac cctgaaacct gaaaccctga aacccagaaa cctgaaaccc cgaaaccctg
      241 aaaccaaaga gcgttgcttt caacgctctt tttaggttcc cggtagggac gcatccccga
      301 ggcgtccgtg cgattgggtc acagccgcag atcgtccggc ggcggcggcg gttccgcgga
      361 caacatttcg gcttctgtgg tgacgccccc ttcactttcg tagagggtgt ggtaactgca
      421 gaccagcata cagtagctca gcggcagggt gaaaaagatg ccgacgccgc aggcgacaaa
      481 gcccagtccg ccgatcaggc tgaccagcgc gccgaacagg aacggcatgg tgaagtcgcc
      541 ccgcccgata tcgccgccga ccgccttcag ggcctccacg gccgtctcgc gccggtacac
      601 gatgcgcatc atcgaccaca tcaggaccga ccccaccagc aggcccgcga ggtgtccgag
      661 caccggcacg agcgacagga caaaggagat cacgaaacag atcagactca gcaccagggc
      721 ctgcataaag aagtcgaggc ccttgaacac atccccggcc tgcggacgga ccggatcttc
      781 ccggatcaga cggtccagca cgagcagcag gcccgcgaag agcggtccgg ccagaatgcc
      841 gcaggtcagc gcacccacaa ccgtgcagat caggcccgcg aggaccagca tccccatgtt
      901 tgcccgaaaa aggcgccatc ccctgcccat ctccgcgccc atatccagtt tcaacgctgc
      961 catatcccac tccattctgt gcccacggca cgtgtgtgtt gtttatcaca cgaagccacg
     1021 aaggccataa aagggaatgt tgtcatgaaa cccctttgca tcctttgtgc ctttgtgtga
     1081 gatgaaatcg ggttgagggc tcaactcaaa ccttgagatc gaagccggcg cggggggcgt
     1141 cggccagcca gcggttggct tcctcatggt tcgagaaccg gttccgggcc gaatcccact
     1201 tcagttccac gccgggatag cgcagcgcga tgcagccgag cagcagcgtc tgggtcatgg
     1261 gaacggagta ttcgaactgg cttcccggcc gggtcatgtc gttctctttg cacgcctcga
     1321 cccactgccc gtaatggctg cagcccttgg tctgtttcag ctcttcgcgc cagtggcgct
     1381 cggcgtcctt gaccgcgggc ccgtaggcct gcccgcccag cgcgatcggc atgggcggcg
     1441 cagcgtgcga accgcccatg acggacattt tggaaccgat gatcaggcag ccgttggtgc
     1501 cgggtttcag ttccggatgc gcgttcgcgg gcagggtcgg cagtttcttg ccgtcgtacc
     1561 aggtgacctt cacgcccttg gggcagaccg gcgtggccgg gaaggtgaat tcgatgatcg
     1621 accaggcggg gtaggcgatg ccggccggcg aggaggcttc ggcccggatc gcggcgggca
     1681 tgccgagctg caggatccag aaggcggggt ccatgttgtg gcaggccatg tcgccgatcg
     1741 cgccgcagcc gaaatcccac cagccgcgcc acttgaacgg cacgtaggcg gtcgagaacg
     1801 ggcgcatgga ggccggaccg atccacgaat cccagtcgaa cccttcggga accgggtcct
     1861 ctttgggctt ctcggtcatg ccctgcggcc agatcggccg gtcggaccag gtgtgcacct
     1921 gctcgatctc gccgaaggcc ttggcgtcca tcatcttttt ccacaggatc aggcccggac
     1981 cggcgtgccc ctggttgccc atctgggtga ccaccttgta tttgcgcgtg gcctccagca
     2041 gcagccggca ctcctcatag gtgcgggcca gaggtttctg cacgtacacg tgcttgccgc
     2101 ggcgcatcgc gtccagagcc gcgcaggcgt gcgtgtgatc cggcgtggag atcaccacgc
     2161 cgtcgatctc cttttccagc ttgtccagca tcttgcggta atccgtgaac ttcggcacgt
     2221 cgggatagct ctggtacgcc ttctccgcgc gcttggggtc cacgtcgcag aggccgacca
     2281 cgattccgcc ggaggagacc gtgctgttga gatcggctcc gcccatgccg ccgcaaccga
     2341 ttttggcgaa ccggaactgc ttgcccggcg gcgcgttgag ggtctggccg aagagggagc
     2401 ggcgggggat cgtgaacgtc gcgagcgcgg cggaagaggc gaggaaacgg cggcggctga
     2461 aaccaaaggc gtgacgcatg atgaaggatc ctttctgtgt gatgtccatg aaaccgttat
     2521 cgggtatagt gtaataacga tgcggcaggg gggcaagcca caaatcaacg caagcgggcg
     2581 cgcaacgcct gctcccggtc cgcatcccag taggcgcagt ccagctcgat gaaaaccgac
     2641 gaatacggga tcacgcagtc ggttttcagc accacgacgt ggaagagctt cgaggccgcg
     2701 tcgagtttcg cgaagaccga ttcgtgcggc agggtctgga ccccgtcggc aggaagcacc
     2761 gcacgggtcg cggcgcggaa cgcgtcgatg cccggaacca gctcctcggt gagagccccc
     2821 aattcgaggt cggtgtaaaa aatgggcttc acatgcgcgg agttccggag ggaggtcgcc
     2881 acgcgggcga gaacctcggg caacggcgcg cgggtgtcaa aataggtgat gcccggcgcg
     2941 ctttgatagg ggtaggcctg atccgtgacc aggatccagt tgcggtggcc gagcaacggc
     3001 agcagccgat cgaattcctc tgtccagttc ttcatgcgtt ctcctgaggg gttgcgggtg
     3061 gatgcggagt ccgcgcaccc cgtgccgaaa aggacacagg ctgacgcaaa cagcgcggaa
     3121 aggggaattt tcatgcgagg tctccgatca ggctgactat ctcgcgccac gagccggtgc
     3181 ggcgcatgtt gggccggtgg gcgtagtgcc ggttccacgg ccggtcatag acgatgaccg
     3241 tgcagtcgcg gcgcggcgcg agcagatcaa gggccgcggg cgaatcgtcg atcgcgacat
     3301 caaaggacat gtcggcgaac tcctcgcggc tgagggtccg gggcgtgtcc ggcgtcggtt
     3361 gtcccgaggc gcgcccgtac ttgtcgacat acagaagctc gaggtgcgcg aggccgtgtt
     3421 tcctcagcca tccgcacgat ccgctgtggc tggaggcagg gcgcccggtg acgatcatga
     3481 ggtcatggcc gcgcgtctcc agttcgcgga ccccttgcag accgcccggg gcgggcggga
     3541 gcgcctcgag gaacgccgtc tggtgcgcgc gctccatcag cagggcgatt tccgcatccg
     3601 tgaggccgaa cgcctgctgg agatcgaacc cggtgatcgc ttcgtacggc acggtgctct
     3661 ggaacaactc acccgccagc gccgcgagcg gacgcgcggt ctcgcacaac acgtcgtcga
     3721 agtcgatgta gatccgcata cccgtaccat agcacaatac gccggttacg caaagccgcg
     3781 gactgcgcca aaaaagagaa cgcgcaacgc tcgggaacgg ggacttgcga catgcgcccc
     3841 gtgaccatcg gcaggaacgc gggggcgcgg accgggtttc ggaccctgaa tgccgcactt
     3901 tccggtagtc aggcatccga aaaaaccgtt atcctatggg ttctggaaca tcaggagaaa
     3961 aatatggcca ctttgatcga cccgtccacg ctgaaaatca ctgacgtccg tttcgccgat
     4021 atcgacggcg cgcccaagcg ttgcacgctc atcaaggtct acaccaacca agggttgatc
     4081 ggctacggcg aggtgcgcga cgcttcgagc cgcacctacg ccgcgatgct caagagccgc
     4141 atcctcggcg agaatccctg caacgtggag aagatcttcc gccgcatcaa gcagttcggc
     4201 ggccactcgc gtcaggccgg cggggtctgc ggcatcgagg tcgcgctctg ggacttggcg
     4261 ggcaaggcgt ggggcgtgcc ggtgtggcaa ctcctcggcg gcaagttccg
//
LOCUS       DAOSLE010000192         4303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_18076_length_4303_cov_7.444915, whole genome shotgun sequence.
ACCESSION   DAOSLE010000192 DAOSLE010000000
VERSION     DAOSLE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18076_length_4303_cov_7.444915"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..4158)
                     /locus_tag="PK576_09930"
     CDS             complement(196..4158)
                     /locus_tag="PK576_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61948.1"
                     /translation="MKRFSWTFVAVALGMVLHAYDDAEIKVSAKIWDATDTNVWQRAV
                     PDFGPGSAVTFSGADSQYLYFGTNVTIGSLTVPNTVTPGSRAYYNIYPWRERWATSDD
                     PTWAGMKWGERGYTLTMDSGSEAPAVIGAFAPLAGNIYRLGCHVPIKMASDLKLVHNG
                     RTDWPGGSSGNGENVSLWMLGDISDDGGTHMLITEGPRYFTGKTWAHPVIALCGDNTF
                     SGDVDIRAWTRLLNKRGIHDETSGTQLGRDNCVYVTNDCGVLDLSGNTYGVDHHLYIG
                     GYTAFTSTVGKLTSMYGNPNATAVWKGPVTLVGDTAFGGAFSTYAILSMSSNMRIEGD
                     IDDDGNNRNLQLVCPNRITLAGKNTFGGNLYHNTGFLTIEHPEGFSGGGKLVLASDAV
                     YRMTAPNQPDLSMILYTNTVERHKIRLRLDGDFTYTPQSGFTNFTEFYKSGPGTLVWD
                     KTNKGIGGNSNMDFFCLDGDTIFDYTQNDTLRLSGHTYFATIGAGNGGRVVLKTKNNQ
                     KNICDFAGVDWSTGEIVKEGAATYKAATPIKNAPYLLNFIVRDGMIIGEKSRQPILGA
                     SASLPNTYGESLIWSNRTWVSAVDPDVANPVWCGFDAYTNDWTRGKCFIDVTPELLET
                     PIPPDFSVAALRFNTPPAGDTMTLTLSGTNDITGGCILITPEFGNDKTLRITGGAIGR
                     PTAGTMRIVSYATNTVVILESDLVQTNRANAVNFCVYHCKVQFTGDSDYSGDVGIVGG
                     ELEVWDNSFMGGLASAGNINRGIHLNQGARLTTHGDFTLRRTVGSYYVIPYFRVYEKG
                     GVLNVADGELATGESTGEIALYDNAAFTKTGKGHLNWNGGMARAGGTCMIGADKIHRY
                     TFTDGKVTMNGNGFTNFRESQNLITVMSNVVITGDCAFNSNLGGVNGGGNAVDCGAKT
                     AFYVGEGGVTFDVAGVARTVGNDGGDKAGFSGHPVGDFYVGSGDITFTNTSETAGSIV
                     FKELGLSRLTGRMIQCAPKQNSGDCTPPFLLPQAEYVVPADLDTYVYQMMDGYQDLWI
                     GRLTGEGAFRIYGRNNYMDPVAHIGRDADETFTFGGRLCAFSISENNNASARYMKIGT
                     NRWRLTNPAGEVRSSTRVANGDVLIACEPSDGEAGALGFCDIYLGDTKTPPNGVPRVL
                     TDGAYTITNHFRILASAPATACPALGGNAVTEASTFTGDIRLWHDIDLYSAGGTVTFT
                     GAFLNGDGNPHAIAKRGPGKVIIQHALPAFSAVNIAEGILELTAPFAIPEGATLDFDT
                     SVCTKENRHNIYTLLACPGGVSGRFEVIADIPFDWKVIYANDAIHLVWAKRGTLIQFH
                     "
BASE COUNT          911 a         1249 c         1185 g          958 t
ORIGIN      
        1 gcaggccatg cgatatttgt ggcaaggaca cctgccacga ccagaaggat gataacaaat
       61 gatttcatgt caatcgccac atgaaggtta tcatgggagg ataatatcag gcctccccgt
      121 gtgttggcaa agcgctggcg aaccaattgc gaagcgttgc ggcgacacgg aggcgcaaca
      181 ctccgcaata aggactcaat ggaactgtat cagggtgccg cgcttagccc aaacgagatg
      241 gatcgcatca ttggcataga tcaccttcca gtcgaacgga atgtctgcga tcacctcgaa
      301 gcgtccactg acaccaccgg ggcacgctaa aagcgtgtag atgttgtgac ggttttcctt
      361 tgtgcagacg gacgtgtcga agtcgagcgt cgccccttcc gggatcgcga atggcgctgt
      421 gagttcgagt atgccttcgg cgatgttcac tgcggagaag gcgggtaacg cgtgctggat
      481 gatcactttg cccggtcccc tcttcgcaat cgcgtgcgga ttgccgtcgc cgttcagaaa
      541 tgcgcccgtg aacgtcaccg tgccaccggc ggaatagagg tcgatatcat gccacaggcg
      601 tatgtcccct gtgaaagtcg aagcttccgt caccgcattt ccgccgaggg ccggacacgc
      661 tgttgcaggg gcactcgcga gaatacggaa gtggttcgtg atcgtatatg caccgtcagt
      721 cagaacacgc ggtacgccgt tcggcggcgt cttcgtgtca cccagataga tatcgcagaa
      781 gccgagtgcg ccggcttctc cgtccgacgg ctcgcacgcg attaacacgt cgccgttcgc
      841 gacgcgcgtc gaagagcgca cttcgcctgc gggattcgtg agacgccacc ggtttgtacc
      901 gatcttcata taccgtgcac tggcgttgtt gttctcggag attgaaaatg cgcagagcct
      961 gcctccgaaa gtgaaggtct cgtctgcgtc gcgtccgatg tgagccaccg gatccatata
     1021 attatttcgc ccgtagatac gaaacgcgcc ctcgcccgtg aggcgtccga tccagagatc
     1081 ctgatacccg tccatcattt ggtaaacata cgtgtccaag tcagccggca ccacgtattc
     1141 cgcctgcggc aggaggaacg gcggcgtgca atcgccactg ttctgtttcg gtgcgcactg
     1201 gatcatcctg cctgtgagac gcgacaagcc gagttctttg aatacaatgc ttccggctgt
     1261 ttcggatgtg ttggtgaacg tgatgtcacc ggaacccaca tagaagtccc cgaccggatg
     1321 gccagagaaa ccggccttat caccgccatc gttaccgact gttctcgcga cccccgcgac
     1381 atcgaacgta acaccccctt cgcccacata gaacgccgtt ttcgcgccgc agtcaaccgc
     1441 gttgccaccg ccgttcacgc cgccgagatt ggagttgaac gcgcagtcac ccgttatgac
     1501 gacattgctc attacggtaa tcaggttttg ggactctctg aagttcgtga acccattccc
     1561 gttcatcgtc accttaccat ccgtaaacgt ataccggtgg atcttgtccg caccgatcat
     1621 gcacgtaccg cctgcgcgtg ccatcccgcc gttccaattg agatgtccct tgccggtttt
     1681 cgtgaacgcg gcgttatcat agagagcaat ttcaccggta ctttcgccgg tggcgagttc
     1741 gccgtcggcg acgttgagga caccgccctt ttcatagacg cggaaatacg gaatgacata
     1801 ataactgcca accgtgcggc ggagcgtaaa gtccccgtgt gtcgtcaacc ttgcaccctg
     1861 attcaggtga atgcctcggt tgatgttccc ggccgaagcc agtcctccca taaacgagtt
     1921 atcccacacc tcaagttcgc cgcccacgat accgacgtcc ccggagtagt cgctgtctcc
     1981 cgtgaactgc accttgcagt ggtagacgca gaaattgacc gcgttcgctc ggttggtctg
     2041 cacgaggtcc gattccagaa tgacgaccgt gtttgtcgcg tacgacacaa tgcgcatcgt
     2101 gccggcagtg gggcgcccga tcgcgccgcc cgtgatgcgg agcgttttgt cgtttccgaa
     2161 ctcgggcgtg atgaggatgc aaccgcccgt gatgtcgttc gtgccggaaa gcgtgagcgt
     2221 catcgtatcg cctgcgggcg gtgtgttgaa acggagcgcc gccacgctga aatcgggagg
     2281 gatcggtgtt tcaaggagtt ccggcgttac atcaatgaaa catttgccgc gcgtccaatc
     2341 gttcgtatag gcatcgaacc cgcaccaaac cggattcgcg acatccggat cgaccgcact
     2401 cacccatgtg cggttactcc agatgagcga ttcgccatac gtattcggca gggatgccga
     2461 tgcgcccaag atgggctgac gcgatttttc accgatgatc atgccgtccc gcacgatgaa
     2521 gttgagcagg tagggcgcgt ttttgatcgg ggtggcggct ttatacgtcg ccgcaccttc
     2581 tttcacgatt tcacccgtgg accagtctac accggcgaag tcgcagatgt ttttctggtt
     2641 gttctttgtt ttcaggacca cccgtccgcc gttgcctgcg ccgatcgtgg caaaatacgt
     2701 gtgcccggaa agacggagcg tgtcattttg cgtgtaatcg aaaatcgtgt cgccgtccag
     2761 acagaagaaa tccatattgc tgttgccacc gatccccttg ttcgtcttgt cccatacgag
     2821 cgtgccggga ccggacttgt agaactccgt gaagtttgtg aagccggact gtggcgtgta
     2881 ggtgaagtcg ccgtcaaggc gaagccggat tttatggcgt tccaccgtgt tcgtgtagag
     2941 gatcatcgaa aggtcgggct ggttcggcgc cgtcatacgg taaacagcgt ccgaggccag
     3001 aaccagcttg cccccaccgg aaaacccttc gggatgttca atcgtcagaa accccgtatt
     3061 gtggtagaga ttgccaccga aggtgttttt accggcaagt gtgatacggt tcggacagac
     3121 gagctgcaga ttgcggttgt ttccatcgtc gtcgatatcg ccttcgatac gcatgttcga
     3181 agacatcgat aaaattgcgt acgtactgaa tgcaccgcca aacgccgtgt caccgacgag
     3241 tgtgacgggg cccttccaaa cggcagtcgc gttcggattc ccgtacatgc ttgtgagctt
     3301 gccgaccgta ctggtgaacg cggtatagcc gccgatatag agatggtgat ccaccccgta
     3361 tgtgttgccg gaaaggtcaa gaacaccgca gtcgttcgtt acgtagacac aattgtcgcg
     3421 tccgagttgc gtgccgctcg tctcgtcgtg aatgccgcgc ttgttgagga ggcgcgtcca
     3481 ggcgcggatg tcgacatcgc ccgaaaacgt attgtcaccg cagagtgcga tcacgggatg
     3541 cgcccaggtc ttccctgtaa aatatcgggg accctcggtg atgagcatat gcgtaccgcc
     3601 atcgtccgaa atatcaccca gcatccagag cgaaacgttc tcgccgttac ccgaggaacc
     3661 gcccggccag tctgtacggc cgttgtgcac gagtttcagg tcactcgcca tctttatcgg
     3721 cacatgacag ccgagtcgat agatgttccc cgcgagcggc gcgaaagcgc cgatgacggc
     3781 aggtgcctct gaaccggaat ccattgtgag cgtgtaaccg cgctcacccc atttcatgcc
     3841 ggcccacgtc ggatcgtcag aagttgccca ccgttcacgc cacggataga tgttgtaata
     3901 cgcgcgcgat cccggagtca cggtgtttgg caccgttaat gagccgatcg ttacattcgt
     3961 gccgaagtag agatattggc tatccgcacc cgagaacgtc acagcgctgc ctggtccgaa
     4021 atcaggcaca gcccgctgcc agacattggt atccgttgca tcccaaatct tggccgacac
     4081 cttgatctct gcgtcatcat acgcatgtaa aaccattccc aacgcaactg caacgaatgt
     4141 ccaactgaac cgcttcatgt ccgctccttt tcacgagtga tcaagccgat ggtcacacga
     4201 tttcatagtt agccaacaac caacaaaata tcaaaaacgc aggttataat actaccatag
     4261 atgtggtttt acaaccgatt tcatctgcgg atccacgtcc gtt
//
LOCUS       DAOSLE010000193         4178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_18779_length_4178_cov_8.647344, whole genome shotgun sequence.
ACCESSION   DAOSLE010000193 DAOSLE010000000
VERSION     DAOSLE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18779_length_4178_cov_8.647344"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PK576_09935"
     CDS             <1..695
                     /locus_tag="PK576_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61949.1"
                     /translation="VEPTRLYGFPCFALSVPDQLAHLVHHMLHHLFAVPLRSYLDIAL
                     LMCTRGDALTPEAITAAGTRWQTGTAIPFILRFVADLFDIPLPATLTNSVPQIEATWR
                     DAVLYALARLPHGSERGAESTMLCYRRAGLVGRARLVLGRIFMPRAFLAERYPSARVL
                     AGVPWAWVCRARDLYRTHRATLVALDTDSSPEQQRLDAAAVRTALANHLRKTPDARRQ
                     EAWQNESGRASE"
     gene            692..3574
                     /locus_tag="PK576_09940"
     CDS             692..3574
                     /locus_tag="PK576_09940"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOE61950.1"
                     /translation="MIRSRKKRRVRNMGWMVFVMFGVACARAEPQRGIEDDASALFSV
                     GFEESEAPNLWTAKPLDGAEASIRLETGSGKGYSLRVEKTNVKGYVQWETVPIPVETA
                     TTYRVSAWLNTLHSDRKSKDGTTTAAAVYFMISPFNSKTPGASRSNIFGPTRPFFSSG
                     SQWEKLDMSFTTGPDIDRVRISLVFAQDAIDILMDDLKLTRLGNKKDYKPRYERPVPE
                     TLMPLEEAKKIVAARPRATAEVRRVGQRPRLFLDGKESFPDFYVGNAWRAQNSQVREF
                     GMAGAKVTFIPYILGRGVYGPFGVWSGPGQTDFSEVDEMLWRVLRADPNMYIMFYLCT
                     DPYPEWAAENNLEDVVTDWDGNKAVVNMHFLRWDKVPPPKPDAQKSSYIERYGYVYAS
                     ERLRKDTGEVLRQFAEHIRNSLPGKAVIGYHVIGGNDGQMFQWNDFHGNRCGDYSPAS
                     LRAFQNWLRTKYRRETELKKAWRQPNVTFETAEFPVPERIGTDRLLLDRPEDMDIVDY
                     KIFRSEGTIDTLAGYGQILRDTHKSPILIGTYYAGPVASVISHRATGYLLKKNMFNYV
                     TSVLTYDAIRWPGGTGKAHQAWSSLLLHDTFGLSEADFRSWKTYPGTPENNYHVARVE
                     TEEESSAMIRRDAGHMLAVGQGIWWYDMSGGWFSDPAIMRTITETVNGFRADLKNPAL
                     PRSDVAVFIDERSLSHVASANAAYLDYAGINRQIVLLNSSGVPYHLYLQEDVANRRLP
                     PYKLYLFLNPYHLSDKEWNAIRSLRRDSRMLAFVHAPGIVAGSIPEATDAADAVTRIT
                     GIHVKNGGEQSLQLHTLPGNGIMETPINQINLKAPVFAVDDPEAKPVAAYADGQIAVA
                     FKDFGKWKSLFFGGVSMNEFFLNALAHYAGAWVAAPAGNAVYGSQNFLTIHALYPGNK
                     EVRLLERSEVTDLATGGLISRDTDRLTFPMKRGETRWFHLQPPAKP"
     gene            3746..>4178
                     /locus_tag="PK576_09945"
     CDS             3746..>4178
                     /locus_tag="PK576_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOE61951.1"
                     /translation="MQSLALRLYGENDLRLERFDLPEITDDEILADIVTNSICMSDYK
                     ATIQGARHKRVPKDIAENPIIMGHEFCGNILKVGKNWQHKFRAGQKYGIQPQLNIPGR
                     EHHSPGYSFRWIGGNATRIVIPKEVMELDCLLTYDGDAYYKS"
BASE COUNT          914 a         1301 c         1184 g          779 t
ORIGIN      
        1 atgttgaacc gacacggctc tacggttttc cctgcttcgc cttgtcggtc cccgatcaac
       61 tcgcacatct tgtacaccac atgctgcacc atctgttcgc ggttccgctg cgttcctatc
      121 tggatatcgc gctgctcatg tgcacacgag gcgacgcgct cacgcccgag gcgatcactg
      181 ccgctggcac gcgctggcaa accggaacgg ctatcccgtt catcttgcgt ttcgttgcgg
      241 atctgtttga catacccctg cccgccacct tgacgaacag cgtaccgcaa atcgaggcaa
      301 cttggcgtga cgccgtgctt tacgccctcg cccgcctgcc gcacgggtca gagcgcgggg
      361 cggaatcgac gatgctctgt tatcgccgag ccggtctagt gggacgcgcc cgactggtgc
      421 tgggccgcat cttcatgccc agagcgtttc tggccgagcg ctaccccagt gcgcgtgtgc
      481 tggcgggtgt gccgtgggcc tgggtttgcc gggcgcgtga cctgtatcgg actcaccgcg
      541 ccaccctcgt cgcgttagat accgactctt cgccagaaca acagagactc gacgcagccg
      601 ccgtccgcac ggctctcgcc aaccacctgc gcaagacgcc agacgcccga agacaagaag
      661 cttggcaaaa cgaatcaggg agggcaagcg aatgatacgg agccggaaga agcggcgcgt
      721 ccggaatatg gggtggatgg tgtttgtgat gttcggcgtg gcgtgtgcga gagccgagcc
      781 gcaacggggc atagaagacg acgcctccgc actcttttca gtggggttcg aagagagcga
      841 agctcccaat ttgtggaccg cgaaacctct ggacggtgcg gaggcctcca tccgcttgga
      901 aaccggatca ggaaaagggt attcgctgcg tgtggaaaaa accaatgtga agggatacgt
      961 ccagtgggaa accgtgccta tcccggtcga aaccgcgacc acctatcggg tctccgcttg
     1021 gctgaacacc ctccactcgg accgcaagtc caaagacggg acgaccacgg ccgccgccgt
     1081 ctactttatg atcagcccct tcaactcaaa aactcccggc gcatcccgct cgaacatatt
     1141 cgggccgacc cgcccgtttt tctcctctgg aagccagtgg gaaaaactcg atatgtcctt
     1201 cacgaccggg ccggacatcg accgcgtgcg catcagtctg gttttcgcac aagacgccat
     1261 cgatatcttg atggacgacc tcaaactgac gcgactggga aacaaaaagg actacaagcc
     1321 acggtatgaa cggcctgtgc ccgaaacact gatgccgctc gaagaggcaa agaagatcgt
     1381 ggcggccaga ccgcgcgcaa ccgccgaagt ccgccgggtg ggtcagcggc cgcgcctctt
     1441 tctggacggc aaagagagct tccccgattt ctacgtcggc aacgcgtggc gcgcccaaaa
     1501 ctcccaggtg cgggaattcg gcatggcggg cgcaaaggtc accttcatcc cttacattct
     1561 gggacgcggc gtgtacggtc ccttcggggt ctggagcggg ccggggcaga ccgatttctc
     1621 cgaggttgac gagatgttgt ggcgggtgct ccgggcggat ccgaacatgt acatcatgtt
     1681 ttacttgtgt accgacccct acccggaatg ggccgcggaa aacaacctcg aggatgtcgt
     1741 gactgactgg gacggcaaca aggccgttgt caacatgcac ttcctgcgct gggacaaggt
     1801 gccgccgccg aaaccggacg cccaaaaatc ctcatacatc gagcgctacg gctacgtata
     1861 tgcctcggag cggttacgga aggacaccgg agaggttctc cgccagttcg ccgagcatat
     1921 ccggaactcg cttccgggca aggcggtaat cgggtaccat gtgatcggag gaaacgacgg
     1981 ccagatgttc cagtggaacg atttccacgg gaaccggtgc ggcgactaca gccccgcctc
     2041 cctgcgcgcc ttccagaact ggctccggac aaaataccgg agagagaccg aactgaaaaa
     2101 ggcttggcgt caaccgaatg tgaccttcga gacggctgaa tttcccgtgc ccgaacggat
     2161 tggaaccgac cggcttcttt tggaccgccc tgaagacatg gacatcgtcg actacaaaat
     2221 tttccgcagc gagggaacca tcgacacgct cgccgggtac ggtcaaatcc tgcgcgacac
     2281 acacaaatcg cccatcctga tcggaaccta ttacgcagga ccagtcgcga gcgtgataag
     2341 tcaccgcgcc accggttatc tgttaaagaa aaacatgttc aactatgtga cgtcggtatt
     2401 gacctatgac gcgatccggt ggcccggcgg aaccggcaag gcccatcagg catggtcatc
     2461 gctgctcctc cacgacacct tcggactctc cgaggctgac ttccgctcct ggaagacgta
     2521 tcccggcaca ccggaaaaca attaccacgt tgcacgcgta gaaactgagg aggaaagcag
     2581 tgcgatgatc cgccgcgacg ccgggcacat gctcgccgta ggtcagggca tctggtggta
     2641 cgacatgagc ggtggatggt tcagcgaccc ggccatcatg cggacgatca ccgagacggt
     2701 gaacgggttc cgcgcggacc tgaagaaccc cgccctgccg agatccgacg tggcggtttt
     2761 tatcgacgag cgaagtctca gccatgtcgc ctcagccaac gcggcgtatt tggactacgc
     2821 gggcatcaac cggcaaatcg tgctgctcaa ctcatccgga gtcccctatc acctctatct
     2881 tcaggaagat gtggccaacc gccgcctgcc gccctacaag ctctatctct tcctcaatcc
     2941 ctaccatctt tcggacaagg agtggaacgc catccgatcg ctgcggcgtg acagccggat
     3001 gctcgcgttc gtgcatgctc cgggcatcgt ggcgggaagc attccagagg ctacagacgc
     3061 ggcagacgcg gtgacgcgca tcaccggcat ccacgtcaaa aacggcggag agcagtccct
     3121 gcagctccac acactgcccg gcaacggtat catggaaacc ccgatcaacc agatcaacct
     3181 caaggcccct gtcttcgccg tggatgatcc ggaagccaag cccgtcgcgg cctacgcgga
     3241 tggccagatc gccgtggcgt tcaaggactt tgggaaatgg aaaagcttgt ttttcggcgg
     3301 cgtgtccatg aacgagttct tccttaacgc gctggcacac tacgccggcg catgggttgc
     3361 ggcaccggcg ggcaacgcgg tctacggcag tcagaacttc ctgaccatcc acgcgcttta
     3421 tcccggcaat aaagaggtgc gtctgctgga acgttccgaa gtgaccgact tggcaaccgg
     3481 cgggctgatc tcgcgtgaca ccgaccggct gactttcccc atgaaacgcg gcgagacccg
     3541 ctggttccat ctgcagccgc cagccaagcc gtgacgcagg ccgcaaaact tgacgtggcc
     3601 ctcggcactc caggcttcat ggttccactt gaacgttaag cgcgcggtct ccacttccac
     3661 ttttgccttg tcactccgcc actggaaacg ctaaagttct acccccatta aaaattgtcg
     3721 aaaactctca tgcgaggata ccctgatgca atcattggca ctacgacttt atggcgaaaa
     3781 cgatctgcgg ctcgaacggt ttgacctgcc ggaaatcacc gatgatgaaa tcttggcgga
     3841 catcgttacg aacagcatct gtatgtccga ttacaaggcg acaatccaag gtgccagaca
     3901 caaacgagtt cctaaagaca tcgcggaaaa ccccatcatc atggggcacg aattttgcgg
     3961 caacatcttg aaggtcggca agaactggca acataaattc cgcgctggcc aaaaatacgg
     4021 gatccagccc cagctcaaca tcccgggccg cgagcatcac tcgccgggtt attctttccg
     4081 ctggatcggc ggcaatgcca cgcgcatcgt catcccgaag gaggtgatgg aactcgactg
     4141 cctgctgacc tacgacggtg acgcctacta caagtcgt
//
LOCUS       DAOSLE010000194         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19052_length_4133_cov_7.443109, whole genome shotgun sequence.
ACCESSION   DAOSLE010000194 DAOSLE010000000
VERSION     DAOSLE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19052_length_4133_cov_7.443109"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..335
                     /gene="rpmH"
                     /locus_tag="PK576_09950"
     CDS             198..335
                     /gene="rpmH"
                     /locus_tag="PK576_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOE61952.1"
                     /translation="MKMMWQPSKIKRARKIGFRARMATRNGRAIINARRRKGRIRLTQ
                     V"
     gene            340..762
                     /gene="rnpA"
                     /locus_tag="PK576_09955"
     CDS             340..762
                     /gene="rnpA"
                     /locus_tag="PK576_09955"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOE61953.1"
                     /translation="MSAGDSHDPGRIGNGRRPRLRLFRLDAPAYKRVFAEKRSVYGRS
                     LVMWIAQGADADRRVGVIVSKRNFRRAVDRARAKRLLREAFRLSRPFLAPDADLILIA
                     RTGIGGQTCREVMADFERVCRRARIWRRDADPENKTHA"
     gene            774..1064
                     /gene="yidD"
                     /locus_tag="PK576_09960"
     CDS             774..1064
                     /gene="yidD"
                     /locus_tag="PK576_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOE61954.1"
                     /translation="MGSTATFISRTIRSMLIALVRAYQVVLSPHFGSCCRFEPSCSAY
                     CIEALRTHGVLRGGWLTLKRIVRCRPFGPSGYDPVPSGKQDAVQPEMTGKNV"
     gene            1061..2848
                     /gene="yidC"
                     /locus_tag="PK576_09965"
     CDS             1061..2848
                     /gene="yidC"
                     /locus_tag="PK576_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOE61955.1"
                     /translation="MNKQERIIAFLLGLALVGWMVHSHRMAAKQAREAAERPVATETA
                     APAAPAAPAQAALTAPVATQPQPALPSEPEEVATLRGDRMTVEVSSHGAVLKRVTLDG
                     YYEKPGKPGPDNPPVTFDFAGAPALELSGVPGLADRAAYRVQRHEQECAVTLTAVTPQ
                     GLSVTRRMELSNGYKIRITDQINNTGDSGITLGTNSVQLGAIGRGTSKNELLSIDSLP
                     ASAGAKTRHWGSEKATKSLLAAGASGGMGCGRAPSAVGLADHISVPIHEPQAWIALKS
                     RFFVTLFSASETNCGFVAGMARDTALPTYALKRLSARALFAGRTLAPGESLTRDYTLY
                     VGPKKLSRLQAMGNRMDEIMEFGTFTWFCKLLVPTLNFFHRLIPNYGIAIILLTFLVR
                     ILFWPLTHKSTQSMRKMQEIQPKLKEIQAQFKDNPQKMQQETWAVYRENKVNPLSSCL
                     PMLIQIPVFIALFTVLRSAVELRYASFLWITDLSEPENLFADVLPLPLNILPFLMSGT
                     MALQSYLTPSMGDPQQQKMMMFMMPIMMLVMFYGFPSALSLYWTVSQVLSIIQMLMMR
                     RKGHTPHDGDMTVEPPLTRQQRRQAARAH"
     gene            complement(2888..3532)
                     /gene="eda"
                     /locus_tag="PK576_09970"
     CDS             complement(2888..3532)
                     /gene="eda"
                     /locus_tag="PK576_09970"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004412949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOE61956.1"
                     /translation="MPNVLDRVAEIGVVPVIAIDSVSHALPLADALLAGGLPVAEITF
                     RTDAAAEVIRLLSRERPDLIVGAGTVLSPETMKTAHAAGAKFAVAPGLNPRVAQAAQE
                     LGMPFVPGIANPSDIEAGLDLGCRLLKFFPAEALGGVKLLTALSAPYRHTGVRFMPTG
                     GVTTANLADYLGLPAVAAVGGTWIAKKEEMAAGAWEAVAQRCRDARAVVRQVRG"
BASE COUNT          818 a         1338 c         1238 g          739 t
ORIGIN      
        1 gcgatatctt cgcgcacggt gtccagtatt tgacggatgc gcttgatttc tggacaccgc
       61 gattttggct acagtgtcca tgactatggc ataccgcggg aaacgtccaa aaacggtcaa
      121 tcggttgacc cgctaccggg gactatgtta aagtgttaac tctttttttg actcagacga
      181 aggaggatac cgaaaccatg aaaatgatgt ggcagccctc gaagatcaaa cgggcccgta
      241 aaatcggatt ccgggcccgg atggccaccc gcaacgggcg cgcgatcatc aatgcccgcc
      301 gccgtaaagg gcggatccgc ctgacccagg tgtaaagcca tgtccgcagg cgacagccat
      361 gacccgggcc ggatcggaaa cggccggagg cccaggttgc gtcttttccg gcttgatgcg
      421 cccgcctata aaagggtttt cgcggaaaag aggtcggttt acggccggtc gctggtgatg
      481 tggattgcac agggcgcaga cgccgaccgg agggtcggcg ttattgtttc caaacggaat
      541 ttccggcggg ctgtggaccg cgcccgcgcc aaacggttgc tgcgcgaggc gttccgcctg
      601 agccgccctt ttctggcacc ggatgcggat ctgattctga tcgcccggac cggcatcggc
      661 ggccagacct gccgggaagt gatggcggat ttcgaacgcg tctgccgccg cgcccggatc
      721 tggcggcgcg acgcggaccc tgagaacaaa acacatgcgt gaggaatcaa cggatgggca
      781 gcacggccac attcatcagc cggacgatcc gcagtatgct gatcgcgctg gtgcgcgcgt
      841 accaagtcgt gctttcgccg cacttcggat cgtgttgccg tttcgaaccg tcctgttccg
      901 cttactgtat cgaggcgctg cggacccacg gcgtgctccg gggcggctgg ctaacgctca
      961 aacggatcgt ccgctgtcgt ccgttcggac cctccgggta tgacccggtt ccgtcaggaa
     1021 aacaagacgc cgtccaacct gaaatgaccg gaaagaatgt atgaataagc aagaacggat
     1081 catcgcattt ttattaggat tggccttggt cggctggatg gtgcacagcc accgcatggc
     1141 cgcgaaacaa gcgcgggagg ctgccgaacg ccccgttgcg acggaaaccg cggcccctgc
     1201 ggcaccggcc gccccggcgc aggcggcgct gactgccccc gtggcgacgc aaccgcaacc
     1261 cgccctccct tccgagccgg aggaggtcgc aaccctccgc ggcgaccgca tgaccgtaga
     1321 ggtctcctcg cacggcgcgg tcctgaaacg ggtcacgctg gacgggtatt atgagaaacc
     1381 gggcaaaccc ggtccggaca atccgccggt cacctttgat ttcgccggtg ccccggcgct
     1441 ggaactttcc ggcgtgccgg gtctggcgga ccgcgcggcg taccgcgtgc agcgccatga
     1501 gcaggaatgc gcggtgaccc tgaccgccgt cacgccgcag gggttgagcg tgacgcgccg
     1561 catggagctt tccaacggct acaagatccg catcaccgac caaatcaaca atacgggcga
     1621 cagcgggatc acgctgggca ccaacagcgt gcagctcggc gcgatcggca gggggacgtc
     1681 caagaacgaa ctgctctcga tcgattcgct acccgcttcg gccggggcga aaacgcgtca
     1741 ctggggttcc gagaaggcaa ccaagtccct tctggcggct ggcgcgtcgg gcggcatggg
     1801 ttgcgggcgc gcgccctctg ccgtgggcct cgccgatcac atcagcgttc cgatccacga
     1861 gccgcaggcc tggatcgcgc tcaagtcccg ttttttcgtg accctcttct ccgcctctga
     1921 aaccaactgc ggctttgtgg ccggcatggc ccgcgatacc gccctgccga cctacgccct
     1981 gaagcggctc tccgcccgcg cgttgttcgc cgggcgcacg ctggcgcccg gcgagagcct
     2041 gacccgcgac tatacgctct atgtcggtcc caagaaactc tcgcgattgc aagccatggg
     2101 caaccgcatg gatgagatca tggagttcgg caccttcacc tggttctgca agttgctggt
     2161 gcccacgctc aacttcttcc accgcctgat ccccaattac ggtatcgcca tcatcctgct
     2221 cacctttctg gtgcggatcc tcttctggcc gcttacgcac aagagcaccc agagcatgag
     2281 gaagatgcag gagatccagc cgaagctgaa agagatccag gcgcagttca aggacaaccc
     2341 gcagaagatg cagcaggaga cctgggcggt ttaccgcgag aacaaggtca acccgctctc
     2401 cagttgcctg ccgatgctga tccagatccc ggtgttcatc gcgctcttca ccgtgttgcg
     2461 cagcgcagtc gagctgcgct acgcgtcgtt cctctggatc accgacctga gtgaaccgga
     2521 aaacctgttc gcagacgtgc tgcccctgcc gctgaacatc ctgccgttct tgatgtccgg
     2581 caccatggcg ctgcaaagct acctgacgcc ctcgatgggc gatccgcaac agcagaagat
     2641 gatgatgttc atgatgccga tcatgatgct cgtcatgttc tacggcttcc cctcggcgct
     2701 ctcgctctac tggaccgtaa gccaggtgct ctccatcatc caaatgctga tgatgcggcg
     2761 caagggccat acgccgcatg acggcgacat gacggtagag ccgccgctga cgcgccagca
     2821 acgccgtcag gcggcccgcg cgcattgagg aacgggcgga atacgacgga cggccgcttc
     2881 ccccccccta tccgcgcacc tgccggacaa ccgcgcgggc gtcgcggcaa cgttgcgcca
     2941 ccgcctccca cgcgccggcc gccatctcct ctttcttggc gatccacgtg ccgcccaccg
     3001 cagccacggc gggcagaccc agataatccg ccaagttcgc ggtcgtcacc ccgccggtcg
     3061 gcatgaagcg tacgccggtg tgcctgtagg gtgcggagag ggccgtgagc agcttcaccc
     3121 cgcccagcgc ctcggccggg aaaaatttca gcagacggca gcccagatcc agtccggcct
     3181 cgatgtccga ggggttggcg atgcccggca caaagggcat gcccaactct tgcgcggcct
     3241 gcgccacacg cgggttgaga cccggtgcca ccgcaaactt cgcgcccgcc gcatgcgcgg
     3301 ttttcatcgt ctcgggcgac agcaccgtac ccgcgcccac gatcagatcg ggccgctcgc
     3361 gcgacagcaa ccggatcacc tccgcagcgg cgtccgtgcg aaaggtgatt tccgccaccg
     3421 gcaggccacc cgccagcaac gcgtcggcca gcggcaaggc atgggatacg gaatcgatgg
     3481 cgatcaccgg cacaacgccg atctcagcga ctcggtccaa cacattcggc atgatacgct
     3541 cctttgtctg ctcgtaaaat aaagccattg tcatcatctg acgcgggaga gtcaatcccg
     3601 gactgtgaac cggcacgccc tgcgcgtatt ccccttgatt caaaagagcc tctgtgccat
     3661 catttctata cgtaatgatt agcccgccgc gcgccggagc cgcgaaaaaa ccttggaata
     3721 cgtttaggac atgattaaca tgatgttgtt caatcctgaa aatcctgtca tcctgtcaat
     3781 ttataggcaa ggagacccga aatgagcgag aaagagatca agaccgattg cccctgcaca
     3841 tggccgggat gcccacgaca tggagactgc aaagcctgta aggcctacca ccatgcccgg
     3901 ggcgaaaaaa cgacctgtga gaaacagtag tttgggtgga caaaatggac ggggtggcca
     3961 aaaaggggct ttgcggttat cgcaagctga agagctttca ggtggcgcgg cgggccgctg
     4021 atttcgaaac cgcaagcggt ttcacgaagc gcccctatcg cgcccgcctc cgatttcggg
     4081 gttgactcgt ttcgtcgatc ccccgcataa taggcggcaa tcatgggaac cgg
//
LOCUS       DAOSLE010000195         4108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19185_length_4108_cov_9.034542, whole genome shotgun sequence.
ACCESSION   DAOSLE010000195 DAOSLE010000000
VERSION     DAOSLE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19185_length_4108_cov_9.034542"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..1679)
                     /locus_tag="PK576_09975"
     CDS             complement(180..1679)
                     /locus_tag="PK576_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008724929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE61957.1"
                     /translation="MTNQRENPPMRLAIAYPPLPSEKGVPLLSQNRQFQWFTRPTYIY
                     PVVPAQAATQARAAGHDVAWLDGIASEWTPEEFDARLDAFAPEVVMLETKTPVVRRHW
                     AYIRELKARHPGVRVVLVGDHVTALPRESFEACPVDYVLTGGDYDFLLVNLLEVLRAA
                     GGGLPDTARLEPGVYWRDADGMVRDSGPFRLDHDLKTLPWIDRELTCWPLYAERNGNF
                     RKTPGTYIMSGRDCWHGKCTFCSWTTLYPTYRVRDPVEVVDEIGDLISRYGIREIMDD
                     TGSLPAGAWLKRFCGEMIARGYNRKIRIDCNMRFGCLDEETYRLMRKAGFRLVLFGLE
                     SANQATLDRLQKGLTVAQIEEGVKWAARAGLDVHVTVMFGYPWEGEGEIANTVRLARS
                     LLRRGHASTLQVTMVVPYPGTPLFRELDQAGLLLTREWDDYDMRRQVMRSGVPEARIK
                     AAVREVYQAFLHPETVVRRLLSTRDPVGDVKFYWRGLLSLVGHLRDFTK"
     gene            1812..2828
                     /gene="ruvB"
                     /locus_tag="PK576_09980"
     CDS             1812..2828
                     /gene="ruvB"
                     /locus_tag="PK576_09980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE61958.1"
                     /translation="MQTRPSEELNRRNASFDNRLRPTGFAEFVGQQKVRERLMLAVEA
                     ARQRGEALDHVLLSGPPGLGKTTLAFILGDAMGVHVKTTSGPVIDKPGDLAGLLTSLE
                     PGDIVFIDEIHRMQRTVEEYLYSAMEDFVIDILIDQGPNARSVRLELPRFTLVGATTR
                     SGLISAPLRSRFGITNRLDYYCIEELSDILTRSAVRLEVSIDEGGTREIAARARGTPR
                     IANNLLRRVRDYAQVRAGNHITQEVAGEALALLEIDPQGLDEMDIRILETLAVKFNGG
                     PVGLNTIAVSVGEEPDTIEEVYEPFLIQEGYLERTPKGRVATPACYRRLGLTPAGRQC
                     ELPL"
     gene            complement(3172..4044)
                     /locus_tag="PK576_09985"
     CDS             complement(3172..4044)
                     /locus_tag="PK576_09985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOE61959.1"
                     /translation="MTDSAASVPCITATLPPEAADALFEIIGDDGFTPTSWYDVESGV
                     CRVSLFPDEPDGVAHTQAALLAAAALLGVTVEPTVTAVAQTDWAESWKRFFHVEKISP
                     RVVVRPSWEPYTAQPGECVITLDPGLSFGTGKHATTQACLRFLDQLAAEDIQRSVLDM
                     GCGSGILAIGAKLLGFTDVRGFDNDPDCMRVSEENAALNGVSIPFTLDDLSQAHPPAD
                     IVVANILAPVLIRFAPQVAGSMSADPRARLVLSGILDTQYDAVCAAYEAQGLVEIDSL
                     LFGEWRSGLFGRGS"
BASE COUNT          840 a         1338 c         1180 g          750 t
ORIGIN      
        1 ctcatctata agggcgtttc acacgacacc cttggttagc cattgcccac agagtaccat
       61 ttgccgcaga aggcgacaag ggccgaaaag ctaaaatcgg ttttattttc ccttccattt
      121 tatagccctt ccaacctccg taacgcgcaa attgtctatg ttccgccata acgggccggt
      181 tatttcgtaa aatcacgcag gtggccgacg agcgaaagca gcccgcgcca gtagaatttc
      241 acgtccccca cgggatcccg ggtggacaac agacggcgca ccaccgtctc cgggtgcagg
      301 aacgcctgat acacctcgcg gaccgcggct ttgatccgcg cttccggaac ccccgagcgc
      361 atcacctgcc gccgcatgtc ataatcatcc cattcgcgcg tcaacagaag cccggcctga
      421 tccaactccc ggaagagcgg cgtgcccgga tacggcacca ccatcgtcac ttgcagcgtg
      481 ctggcatgcc ccctgcgcaa cagggaccgc gccaaccgga cggtgttggc gatttccccc
      541 tcgccctccc acgggtagcc gaacatcacc gtgacgtgca catccagacc ggcgcgcgcg
      601 gcccacttca cgccctcttc gatctgcgcc accgtgagac ctttttgaag acggtcgagc
      661 gtggcctggt tggcggactc caatccgaaa agcaccagcc ggaatccggc cttgcgcatg
      721 agccggtagg tctcttcgtc cagacatccg aaccgcatat tgcagtcgat gcggattttc
      781 cggttataac cgcgcgcgat catctccccg cagaagcgct tcaaccacgc cccggcaggc
      841 aggctgccgg tgtcatccat gatctcccgg atcccgtacc ggctgatcag atctccgatc
      901 tcgtccacca cctcaaccgg atcacgcaca cggtaggtcg gataaagcgt ggtccacgag
      961 cagaaggtac acttgccgtg ccaacagtca cggcccgaca tgatgtaggt tccgggcgtt
     1021 ttgcggaaat tgccgttgcg ctcggcatag agaggccaac aggtcagctc gcggtcgatc
     1081 cagggcaggg tcttgagatc gtgatcgagc cggaaggggc cgctgtcccg caccataccg
     1141 tccgcatcac gccagtagac gccgggctcc agacgcgccg tgtccggcag gccgccccca
     1201 gccgcgcgca ggacctccag cagattgacc agcagaaaat cgtagtcgcc cccggtcagc
     1261 acgtaatcca ccggacaggc ttcgaaactt tcgcgcggca aggccgtgac atgatctccg
     1321 accagcacca cccgcacccc ggggtgccgc gccttgagtt cccggatata ggcccaatga
     1381 cgccgcacca ccggcgtctt ggtctccagc atcaccacct ccggggcgaa tgcgtccagc
     1441 cgcgcgtcaa actcctcggg cgtccactcc gaagcgatgc cgtccagcca agccacgtca
     1501 tgtccggcgg cccgcgcctg cgtcgcggcc tgcgcgggca ccaccggata gatgtacgtg
     1561 ggacgcgtga accactggaa ctggcggttc tgcgaaagca acggcacccc tttttccgag
     1621 ggtagcggcg ggtaagcgat ggcgagacgc atgggcggat tctctctctg gttcgtcacg
     1681 gaacacgcgt tccggattcc cggtcggtct tccggcgggt aaagaacata ttaaaacaca
     1741 gtggccggaa aggggacact caaaaatggc ggcattgttt tccgccccga tttttgtcat
     1801 gataccaatc catgcagaca cgcccctcag aggaactcaa ccgccggaac gcctcgttcg
     1861 acaatcgcct gcgccccact ggcttcgccg agtttgtggg ccaacagaag gtgcgggagc
     1921 gattgatgct ggcggtggag gctgcccgcc agcgcgggga ggcgcttgac catgtgctgc
     1981 tttcgggtcc gcccggactc ggaaaaacca ccctcgcctt cattctcggc gacgccatgg
     2041 gcgtgcatgt caaaaccaca tcgggcccgg tcatcgacaa gcccggcgat ctggccggac
     2101 tgctcaccag tctggaaccc ggcgacatcg ttttcatcga cgaaatccac cgcatgcagc
     2161 ggaccgtgga ggagtatctg tactccgcga tggaggattt cgtcatcgac atcctgatcg
     2221 accaagggcc caacgcccga tcagtccggc tggaacttcc ccgtttcacg ctggtgggcg
     2281 cgaccacccg cagcggcctg atctccgccc cgctccgctc gcgcttcggc atcaccaacc
     2341 gccttgatta ctattgcatt gaggaactca gcgacatcct cacccgttcc gccgtccggc
     2401 tcgaggtcag catcgatgag ggcggcacgc gggaaatcgc cgcgcgggcg cgcggaaccc
     2461 cgcgtatcgc caacaacctg ttgcggcggg tacgcgatta tgcccaagtg cgcgccggga
     2521 accacatcac ccaagaggtg gcgggcgagg cgctcgcgtt gctggagatc gacccgcaag
     2581 ggctggatga gatggatatc cggatcctcg aaacgctggc cgtgaaattc aatggcggcc
     2641 cggtggggct caacaccatc gccgtttccg tgggcgagga gcccgacacc attgaggagg
     2701 tctacgagcc gttcctgatt caggagggct atctggaacg gaccccaaag ggccgtgtcg
     2761 ccacccctgc ctgttaccgc cgcctgggtc tcacccccgc cggccgtcag tgcgagctgc
     2821 cgctatagga ctaatgcggg caaagcccgc aacctcaaag gctgtgagac tgtgaggctg
     2881 tggttaaatg atcgatcccg atcccgatag cgatcccgat cccgatgaaa ccctgaaacc
     2941 aaagagagtt gaaggttcaa ctgctctttt taggataaat tgacaggatt acaggattaa
     3001 catgattttt taggataaat tgacaggatt acaggattaa catgattttt taggataaat
     3061 tgacaggatt acaggattaa catgattttt taaccctgaa aatcctgtca tcctgtcaat
     3121 ttataggcaa gttaattcgt gccttagttt ttccatatgt gaatcttcct gttacgaacc
     3181 tctcccgaac agcccgctgc gccactcgcc gaacaacagg ctgtcaatct caaccagccc
     3241 ctgcgcctcg taagcggcgc ataccgcgtc atactgcgtg tcaagaatgc ctgacaacac
     3301 cagccgcgcg cgcgggtccg cgctcatgga ccccgccacc tgcggcgcga accggatcag
     3361 aaccggcgca agaatgttcg ccaccacgat atcggctggc gggtgcgctt gcgaaaggtc
     3421 gtccagcgtg aaggggatcg acacgccgtt cagcgccgcg ttctcctctg acacgcgcat
     3481 gcagtcggga tcattgtcga agccgcgcac atccgtgaaa cccagcagct tcgcgccgat
     3541 cgccaaaatg ccggagccgc accccatatc gagtacggag cgctgaatgt cctccgccgc
     3601 caactggtcc aaaaagcgga ggcaggcctg cgtggtggcg tgtttgccgg tgccgaagct
     3661 caagccggga tcgagcgtga tcacgcactc gccgggttgc gccgtgtacg gttcccacga
     3721 ggggcgcacc accacgcggg gagaaatctt ctcaacgtgg aaaaagcgtt tccagctttc
     3781 ggcccagtcg gtctgcgcga cggccgtgac cgtcggctcg accgtcacgc cgagcagtgc
     3841 ggcggcggca agcagcgcgg cctgcgtgtg cgccacaccg tccggttcat ccggaaagag
     3901 cgagacacgg cagacgccgc tttccacatc gtaccacgag gtcggcgtaa agccatcgtc
     3961 gccgatgatc tcaaacagcg cgtccgccgc ctcgggcggc agcgtggcgg tgatgcaggg
     4021 aacggaagcg gcagagtcgg tcatggcgtc acatcacacc accgtgcaga gggtgtagtg
     4081 ggcgttgact tcggtcttga tcgcttcg
//
LOCUS       DAOSLE010000196         4104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19205_length_4104_cov_7.317609, whole genome shotgun sequence.
ACCESSION   DAOSLE010000196 DAOSLE010000000
VERSION     DAOSLE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19205_length_4104_cov_7.317609"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..537)
                     /locus_tag="PK576_09990"
     CDS             complement(88..537)
                     /locus_tag="PK576_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOE61960.1"
                     /translation="MKSVPPLECLGYFVTALTVSANPKHNPEAKVELTTDDLTVTTKC
                     VCTDKAARRWHVQLTIRQAVGPDKNAPYNFRLVLLGEFEVVSGFPKEHERRLVETNAC
                     SILMGSAREVLRAAMSSGPYTPLLLPTWSFYQPLKPVTGKKTNKGKR"
     gene            complement(515..1066)
                     /locus_tag="PK576_09995"
     CDS             complement(515..1066)
                     /locus_tag="PK576_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61961.1"
                     /translation="MKRLAPKDETPPFAGLERAKTKLPVIIPAPEGSGLEPRVLEVEV
                     NGWRDPTDGEIYLSGTTTAYVDRIKAHEMGLLAPEDFKLIREQLKVTQEEISSLLQIG
                     KRSWTRWETGRDRPTRSINLLVRSLYDGIITVPYLEELQKPIDHEPLTRWRCTVCPSL
                     YSAPKKQRAQVCEEVSDEVGPAA"
     gene            complement(1063..1425)
                     /locus_tag="PK576_10000"
     CDS             complement(1063..1425)
                     /locus_tag="PK576_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61962.1"
                     /translation="MFAQPAKRVGAIPVAWRQSVCAALAERGECVRLTVRARHEWEAA
                     FPNAFEYELYAVLEATLKKGTVEGRRVEDMRPEGEAYEFFFEFEGRKVYGKINLLTPE
                     RRIVLIISAHIPNKGDTL"
     gene            complement(1465..2646)
                     /locus_tag="PK576_10005"
     CDS             complement(1465..2646)
                     /locus_tag="PK576_10005"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008630167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE61963.1"
                     /translation="MSNLIQKFTVSYRYPVCFTRRVFDPSNQTLAKILKPSASGRARA
                     LVVVDAGVAEVAPGLPQRIERYFRLRCGKIDLVRAPMRVPGGEQAKNGWAGVRALMTL
                     AAETHLDRHSYLVALGGGSVLDMAGFAASLVHRGLRLIRLPTTVLAQGDAGIGVKNGM
                     NSGEVKNAVGTFAPPFAVINDFDFLPTLPQREWVSGIAEAFKVAIIQDRRFFRFLLKH
                     AVALRGRDQALMETLVRKTATLHLRHIATSGDPFEYGSARPLDFGHWAAHRLESLSNF
                     TFGHGQAVAVGIALDSTYAMLNGLISPSERDAILQGLTASGLPVWTPLLERSGANGAP
                     DVLVGLEQFREHLGGKLCVTLPNPLGAKTEVRSIRLPLMRKAIGLLRPGERRMEKLGK
                     A"
     gene            complement(2643..3926)
                     /locus_tag="PK576_10010"
     CDS             complement(2643..3926)
                     /locus_tag="PK576_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOE61964.1"
                     /translation="MRKLLVIQVAGWGHAFSERHGVRRIAGLPVCSSEAVFPALTCVA
                     QAALRTGLDPAGHGILANGFFDRTLRKAWFWEQAATLVEGPRIWEAFRGCGGTVGLAY
                     FQQSLGESADQILSPAPIHKHGGGMILAHYSRPPDLEARLTRAAGGPFRLCRYWGPLA
                     SVGSSEWITRAVAAWLAQPDAPDLVFAYLPGLDYDLQRFGPEHPKSVRALTVVCAEIE
                     RLFAAARANGYEALAFGDYAITPVTHGPVFPNRALRDAGLFAVRAVRGRAYPDFYQSR
                     AFALADHQIAPVFCPDPVALPQTRAVLETLNGVDRVLDSAEQAALGLAHARAGELLLV
                     AKPGAWFAYPWWTDPREAPDYAGHVDIHNKPGYDPCELFFGRMPLRTGADADRIRGTH
                     GLTGAGYETAWVSTVPFSFKTLPLLAASIRAWLAA"
BASE COUNT          839 a         1374 c         1170 g          721 t
ORIGIN      
        1 gctgcctccc aacgttttgc cccgccaccc catacgccct tacgttatcg taaaacgcgg
       61 gccggaacaa ccgcaatcaa gttcaactca acgcttgcct ttattggtct tctttcccgt
      121 aaccggcttt aacggctggt agaacgacca cgtgggcaac agcagaggcg tgtacggtcc
      181 cgacgacatc gcggcccgca acacttcgcg ggctgaaccc atcaagatcg aacacgcatt
      241 cgtctcgaca agcctccgtt cgtgttcctt aggaaacccg cttacgacct caaactcccc
      301 caacaacacc aaccggaagt tgtatggggc gttcttgtcc ggccccacag cctgacggat
      361 cgtcaactgg acatgccagc gccgcgccgc cttgtccgtg caaacgcatt tcgttgtgac
      421 cgtgaggtcg tcagtggtca gctcgacttt ggcctcgggg ttatgcttag ggtttgccga
      481 aaccgtcaaa gcggtgacaa aatagcccag acactcaagc ggcgggaccg acttcatcag
      541 acacctcctc gcatacctga gcgcgttgtt ttttcggagc gctataaaga cttggacaaa
      601 ccgtgcaccg ccaccgcgtg agcggctcat gatcgatcgg cttctgcagt tcctccaaat
      661 acgggaccgt gatgatcccg tcatagaggg aacggacaag caggttgatc gatcgggtcg
      721 gacgatcacg tccggtctcc cagcgtgtcc aggaccgctt tccgatctgc aagagactgg
      781 aaatctcctc ttgggtgacc ttcaattgtt cgcggatcag tttgaaatcc tcaggggcaa
      841 gcagccccat ctcgtgcgcc ttgatccgat caacataagc ggtcgtcgtg ccgctcaggt
      901 aaatctcgcc gtccgttgga tcacgccacc cgttgacctc gacctccaga acccgcggtt
      961 ccagaccgga cccctccggc gccggaatga tcaccggcaa tttcgtcttc gcccgttcga
     1021 ggcccgcaaa cggcggggtc tcgtctttcg gtgcaagacg cttcatagtg tgtctccttt
     1081 attcggaata tgcgctgata tgatcaagac aatacgccgt tcgggcgtta acagattgat
     1141 cttcccgtac accttccgcc cctcgaattc gaagaagaac tcgtaggctt ctccctccgg
     1201 acgcatatcc tctacacgcc gcccttccac cgttcccttt ttcaacgtcg cttccagcac
     1261 ggcatacaac tcgtactcga atgcgttggg aaacgccgct tcccactcat gcctggccct
     1321 caccgtcagt cgaacgcact ccccgcgttc tgccagcgcg gcgcagacgg actgccgcca
     1381 cgcaaccgga atggcgccca ctcttttggc tggttgagca aacataatgt accaattgat
     1441 acgtccggca gcaagccgta aaaatcaagc ctttcccaat ttctccatgc gcctctcacc
     1501 ggggcgcagt aagccgatgg ccttccgcat gagcggcaga cggatcgagc gcacttcggt
     1561 tttcgcgccg agcgggttgg gcaacgtgac gcagagtttg ccgccaaggt gttcgcggaa
     1621 ctgttcgagg ccgacgagca catccggcgc gccgttcgcg ccagaccgtt ccagcagcgg
     1681 cgtccagacg ggcagcccgc tcgcggtcaa cccctggagg atggcgtcgc gttccgaggg
     1741 ggagatcagc ccgttgagca tcgcgtacgt gctgtcgagc gcgatgccga cagctacggc
     1801 ctggccgtgc ccgaacgtga agttcgacag gctctccagc cggtgcgcgg cccaatgccc
     1861 gaaatccagc ggccgcgcgc tgccgtattc aaagggatcg ccggacgtgg cgatatggcg
     1921 caggtgcagc gtggcggttt tgcggacaag cgtctccatc aatgcctgat cgcgcccgcg
     1981 caaggctacg gcatgcttca ggagaaaacg gaaaaagcgc cggtcctgaa tgatcgcgac
     2041 tttgaacgcc tccgcgatgc cgctgaccca ctcgcgctgc ggcagggttg gcaggaagtc
     2101 gaaatcattg atcaccgcga acggcggggc aaaggtgccc accgcatttt tcacctcccc
     2161 gctgttcatg ccgttcttca ccccaatgcc cgcgtcgccc tgcgccagca cggtggtcgg
     2221 cagacggatc aggcgcaggc cgcggtgaac cagcgaggcg gcgaaacccg ccatgtccag
     2281 cacgctgcca ccgcccaacg cgaccagata actgtgccgg tccagatggg tttccgcggc
     2341 aagggtcatc agcgcgcgta ccccggccca cccgttctta gcctgttctc cgcccggaac
     2401 acgcatcgga gcgcgcacca gatcgatttt cccgcaacgc agacggaagt accgctcgat
     2461 acgctggggt agccccggtg cgacttcggc caccccggca tccaccacca ccaacgcacg
     2521 cgcccggccg ctggccgacg gtttcagtat cttggccaga gtctggttgg acggatcgaa
     2581 gacccgtcgt gtaaagcaca ccggataccg atacgaaacc gtaaatttct ggatcaggtt
     2641 actcatgcgg caagccacgc ccgaatggat gctgccaaaa gcggcaaggt tttgaatgag
     2701 aacggcacgg tcgagaccca tgcggtttcg taacccgcgc cggtgagtcc gtgcgtgccg
     2761 cgaatgcggt cggcatcggc gccggtccgc agcggcatgc gcccgaaaaa gagttcacag
     2821 ggatcatagc cgggtttgtt gtggatgtcc acatgacccg cgtaatccgg tgcttcacgg
     2881 ggatcggtcc accagggata ggcgaaccat gcgccgggtt tcgccaccag caacagttcg
     2941 cccgcgcgcg catgggccaa cccgagcgcg gcctgctctg ccgagtccag tacccggtcc
     3001 acaccattca gcgtctccag aacggcccgc gtctgcggca gggcgacggg gtccgggcag
     3061 aacaccggcg cgatctggtg gtcggcgagc gcgaaagcgc ggctctggta aaaatccgga
     3121 tacgccctgc cgcggaccgc gcgcacggcg aacaaccccg catcacgcaa cgcacggttc
     3181 gggaaaaccg ggccatgcgt caccggcgtg atcgcgtaat ccccgaaggc cagggcctca
     3241 tacccgttgg cgcgagccgc agcaaagagc cgttcgattt ccgcgcaaac cactgtaagc
     3301 gcccgcacac ttttcggatg ctccggcccg aaccgctgga gatcgtaatc gagtccgggc
     3361 agatacgcga aaaccagatc cggcgcgtcc ggctgcgcaa gccatgcggc cacggcccgc
     3421 gtgatccact ccgaagaacc gaccgacgcg agcggccccc aataacggca caggcggaac
     3481 gggccgcccg cggcccgggt cagccgcgct tccaagtccg gcggacgact gtaatgcgcc
     3541 aggatcatcc cgccgccatg cttgtgaatc ggcgcgggcg agaggatctg atccgccgat
     3601 tctccgagac tctgctgaaa gtaggcgaga cccacagtcc cgccgcagcc acggaacgcc
     3661 tcccaaatcc gcggcccctc aaccagcgtg gccgcctgct cccagaacca ggctttgcgc
     3721 agggtccggt caaaaaaacc gttggcgagg atcccatgcc cggccggatc gagcccggtg
     3781 cgcaacgcgg cctgagccac acaggtcagt gccggaaaaa ccgcctccga agagcagacc
     3841 ggcaaccccg cgatcctccg aacgccatga cgttcggaga acgcgtgccc ccatccggcg
     3901 acctgaataa ccagcagttt gcgcatgtcc tgtttcccgt tttcgacgtc ggcaccgcgt
     3961 tgagccgacc cgatccgctg aagtcagaag tataatgcgc cccgttaccg atgacaagcg
     4021 cgacaccaac aaccaagggg cattgaaccc aaaaggagca gttgaacggg tagaattcta
     4081 gcaaagcccc gtaaacattg ggtt
//
LOCUS       DAOSLE010000197         4089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19278_length_4089_cov_7.715419, whole genome shotgun sequence.
ACCESSION   DAOSLE010000197 DAOSLE010000000
VERSION     DAOSLE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19278_length_4089_cov_7.715419"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1413)
                     /locus_tag="PK576_10015"
     CDS             complement(190..1413)
                     /locus_tag="PK576_10015"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOE61965.1"
                     /translation="MIRVNEHYAKLQSSYLFSEIAKRVKAYQAEHPDKRILRLGIGDV
                     TEPLPPACVAALHQAVDEMASRSSFRGYGPEQGYDFLREAIARNDFQARGAEITPQEI
                     FVSDGAKCDTANIQELFTDKLTLAIPDPVYPVYVDTNVMAGRTGKCVKGRYKGLVYLD
                     STESNGFVPDLPEQPVDLIYLCFPNNPTGATATREQLAAWVRYARENRALILFDAAYE
                     AFIRDPALPHSIYEIEGARDVAIEFRSFSKTAGFTGTRCAYTVVPTSTMAYTKKGEPV
                     ALNPMWNRRHTTKFNGVAYPVQRAAEAVYSAEGQAQTRALTDFYLGNAARIRAAFTEL
                     GYSLTGGENSPYIWVNVGKDSWKFFDKLLKRAQVVTTPGAGFGRCGQGYIRISAFNSR
                     ENVEEAMMRLRKVLK"
     gene            complement(1410..2360)
                     /locus_tag="PK576_10020"
     CDS             complement(1410..2360)
                     /locus_tag="PK576_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE61966.1"
                     /translation="MPIRLQHRIEYAALRAAETILNALPYRVALGTAWLAAFFLFTVL
                     RFRRQETFRRIREVFGGDYPSRAVRHIAWRSLRNMTFNVVELMRAPAVDRAWIDRHLP
                     TFATYVPEVQEQIRQYGGAVITVPHMGNWDLAGWACHHYGISMFSIAGKQKNPLVNAW
                     INRRRECGMTILERGGGTLRQIRTLLREGSVLAILPDVRVYTPDLEVAFLGGKANVGR
                     GMARFAMAAGVPVIPAILRREGWTRHGFDLLPSILPDPSREREAEARRITETVLAHVD
                     AAIRRTPDQWFWYNKRWVLAPVGKPSRPQAATSGTERENP"
     gene            complement(2481..3863)
                     /gene="pcnB"
                     /locus_tag="PK576_10025"
     CDS             complement(2481..3863)
                     /gene="pcnB"
                     /locus_tag="PK576_10025"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOE61967.1"
                     /translation="MFFQKAYPIVRERKDHCLSRANIAPEALKVLYRLIRAGYTAYLV
                     GGSVRDLLMGRQPKDFDISTNARPNEIRRLFRNCFLIGRRFRLAHIVFGHKIIETSTF
                     RRQPDSNDGDGGSGIYQTEDNTFGSPEEDAKRRDFTVNGLFYDPQSFSVIDYVGGIRD
                     LEKRVLRSIGDPDIRFREDPVRMMRAVKFAARLDFSLDRACRKAILKHHADILNASVP
                     RLCEEVFRLFTYGTSRTAFRLCWEYKLLGDLMPELDAFISRNGGANASLWNVLAALDA
                     DPQSADLTNGVRVACLYYPLFRAGLEQEEARYPRKRANRQHVARRILQPLAHRLHIPK
                     ATLYSAITLLDAGRRFAEPPQKGRSLRFLHHPAFPEALAFYRIISAAEKTDRSRLDAW
                     QALYDAEVAQRKANGSRDAESDPPARARRRRRRKPRTDRAATAPTQPSRHPVTVTEVP
                     SAKADTRTDS"
BASE COUNT          742 a         1169 c         1369 g          809 t
ORIGIN      
        1 cggtgatccg cgtcacggac agcgtggaga tcctcggaaa gggggcgggc agcccgcccc
       61 aagccgccac gccggaaacg ccgaagccgt gaggctgggt tacgagacct tcgggagttt
      121 gtggaaaccc gttcggacga ggtcgtggcc aatgcggctg gaaacacccg gcgccaccca
      181 ttccccgggt cacttcagca ccttgcgcag ccgcatcatc gcctcttcca cattttcacg
      241 gctgttgaag gcgctgatgc ggatgtatcc ctggccgcag cggccgaaac ccgcaccggg
      301 cgtggtcacc acttgcgcgc gcttcagcag tttgtcgaaa aacttccagg agtccttgcc
      361 gacgttgacc cagatatacg gactgttctc gccgccggtc agagagtatc cgagctcggt
      421 aaaggcggcg cggatccgcg cggcgttgcc gagatagaaa tcggtcagcg cgcgggtttg
      481 agcttggccc tcggcagagt aaaccgcctc ggcggcacgt tgcaccggat aggccacgcc
      541 gttgaatttg gtggtgtgac gccggttcca catcgggttc agcgcgacgg gctcgccttt
      601 tttggtgtag gccatcgttg acgtcggcac caccgtatac gcgcagcgcg tgccggtaaa
      661 gcctgcggtt ttggaaaaac tgcggaattc gatggccacg tcccgcgcgc cctcgatctc
      721 gtaaatactg tggggcaggg ccgggtcgcg gataaacgcc tcgtaggcgg catcgaacag
      781 aatcaacgcc cggttttcgc gggcatagcg cacccaggcc gcgagttgtt cgcgggtggc
      841 ggtcgcgccg gtcggattgt tcgggaaaca gaggtagatg aggtccaccg gctgctcggg
      901 caggtcaggg acaaagccgt tcgattctgt gctgtccaga tataccagcc ctttatagcg
      961 gcctttgacg catttgccgg tgcgtcccgc catgacgttg gtgtccacat agaccgggta
     1021 gaccgggtcg gggatcgcga gggtcagctt gtccgtgaaa agctcttgga tgttggcggt
     1081 gtcgcacttc gccccgtcgc tcacaaagat ctcctgtggg gtgatctccg cgccgcgcgc
     1141 ctgaaaatcg ttgcgggcga tcgcctcgcg caggaagtcg tatccctgtt ccggtccgta
     1201 tccccggaac gaggagcgcg acgccatctc gtccacggcc tgatgcaggg ctgccacaca
     1261 ggcggggggc agcggttcgg tcacgtcgcc gatgccgagg cgcaggatcc gcttgtccgg
     1321 atgctcggcc tgatacgctt tgacgcgctt ggcgatctcg gaaaaaaggt aggaggattg
     1381 gagtttggcg tagtgctcat tgacgcggat catgggtttt ccctttcggt tccggatgtc
     1441 gcggcctgcg gtcgggacgg tttgccgacc ggggcgagca cccagcgttt gttataccaa
     1501 aaccactggt cgggtgtccg ccggatggcg gcgtcgacgt gggcaagaac ggtttccgtg
     1561 atgcggcgtg cctcggcctc gcgttcccgc gaagggtctg gcagaatgga gggcagcaga
     1621 tcgaacccgt ggcgggtcca accttcgcgc cgtaggatgg cggggatcac cgggactcct
     1681 gcggccatgg cgaaacgcgc catgccgcgt cccacattcg ccttgccgcc gagaaaggcg
     1741 acttcgagat caggcgtgta gacgcggaca tccggcagga tcgccaacac gctgccctcg
     1801 cgcagcaagg tccggatctg gcgcagtgtg ccgccgccgc gttcgaggat cgtcatgccg
     1861 cactcccggc gccggttgat ccatgcgttg accaacgggt ttttctgctt tccggcgatg
     1921 ctgaacatgc tgatgccgta gtggtggcag gcccatcccg ccagatccca gttgcccatg
     1981 tgcggtaccg tgatgaccgc gccgccgtat tgccggatct gttcctgaac ttccgggacg
     2041 tacgtcgcga aggtcggcag gtggcggtcg atccacgcgc ggtccactgc gggcgcgcgc
     2101 atcagttcca cgacattgaa cgtcatgttg cggagggatc gccaggcgat gtgccggacc
     2161 gcgcgggaag gatagtcccc gccgaacacc tcgcggatgc ggcggaaggt ttcctgtcgg
     2221 cggaaccgga ggacggtgaa gagaaagaag gccgcgagcc aagccgtgcc cagcgcgacc
     2281 cgatagggca gcgcgttcag gatcgtctcc gccgcccgca gggcggcgta ttcaatgcgg
     2341 tgctggagac ggatgggcat gtcgggtggt tgtctagcca gaattacagg attaacagga
     2401 tttggattaa gcggggtaca tcatgcaaat cgtgttaatc cgtaatcttg gctaaaaaat
     2461 gatctctttc gttttgctgg ctaactgtcg gtacgcgtgt ccgcctttgc tgacggcacc
     2521 tccgtgacgg tcacggggtg acgggatggt tgcgtcggcg cggtggcggc acgatcggta
     2581 cggggcttgc gacggcgccg tcgccgcgcg cgcgcaggag gatcgctctc cgcatcgcgg
     2641 gatccgttcg ctttgcgctg cgcgacttcc gcatcgtaga gggcctgcca ggcgtccagc
     2701 cgggaacggt cggtcttttc ggcggccgag atgatgcggt agaacgcgag cgcctccgga
     2761 aaggcggggt ggtggagaaa acgcagggag cgtccttttt gcgggggttc cgcaaaacgg
     2821 cgtccggcgt ccaagagcgt gatggccgaa tagagggtcg ctttggggat atgcaggcgg
     2881 tgcgcgagcg gttgcaggat ccgacgcgcc acatgctgac ggttggcccg tttgcggggg
     2941 taccgcgcct cctcctgctc gaggcccgcc cggaagagcg ggtaatacag gcaggctacc
     3001 cggacgccgt tggtcagatc cgcgctctgc ggatcggcat ccagcgcggc gagcacattc
     3061 cacagcgatg cgttggcgcc gccgttccgg gagatgaagg catcgagttc cggcatcaga
     3121 tcgcccaaca gtttgtattc ccagcagaga cggaatgcgg tgcgcgaagt gccgtaggtg
     3181 aagagccgga acacctcctc gcacaagcgg gggaccgagg cgttgagaat gtccgcatgg
     3241 tgtttgagta tggctttgcg gcaggcgcgg tccagcgaga aatccaggcg cgcggcgaac
     3301 ttgacggcgc gcatcatgcg gaccggatcc tcgcggaacc ggatatccgg gtcgccgata
     3361 ctgcgcagca cccgtttttc gaggtcccgg atccccccga cgtaatcgat cacggaaaag
     3421 gactgggggt cgtagaacaa accgttcacg gtgaaatcac ggcgcttggc atcttcctct
     3481 ggcgagccga aggtgttgtc ttccgtctgg tagatgcccg agcccccgtc cccgtcgttc
     3541 gagtccggct ggcgccggaa ggtggaggtt tcgataattt tatggccgaa aacgatatgg
     3601 gccaaacgga aacggcgtcc gatcaggaaa cagttgcgga agagccgccg gatttcgttc
     3661 ggacgcgcat tcgtgctgat gtcaaaatcc ttgggttgtc ttcccatcag gaggtcgcgc
     3721 acactgccgc ccaccagata ggcggtgtat cccgcacgga tcaggcggta gaggacttta
     3781 agagcttcgg gggcaatatt cgcgcgggag aggcaatgat ctttgcgttc gcgcacgatc
     3841 gggtaagctt tttgaaaaaa catggttttt agtcattcac cggttgcaaa ccgaatcgtc
     3901 ataacagaat taacgaagta tgccaaaaaa ccggcgtcga aacaagcggc ggaatgcacg
     3961 ttgcgcggtt gaaccttcaa ctctctttgg tttcaaggtt tcagggtttc tggtttcatg
     4021 gtttctggtt tcatggtttc atccaaatcg gggtcgggat cgctatcgct atcgggatcg
     4081 attgtttaa
//
LOCUS       DAOSLE010000198         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19459_length_4060_cov_6.908864, whole genome shotgun sequence.
ACCESSION   DAOSLE010000198 DAOSLE010000000
VERSION     DAOSLE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19459_length_4060_cov_6.908864"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PK576_10030"
     CDS             <1..1213
                     /locus_tag="PK576_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61968.1"
                     /translation="TFYMNVPQATPQYTGRRYFEWRSGYSVSNEMVVSGSSVASGFST
                     FDMNGAWDLLRIQDTAKASPPVVMNGVSNRVEVLGGELSSGSCQVNGRYNRLLLSDGL
                     LVTGNKGTLTMGGNGPNLIRQTGGTCSAPDGVTIGGFSNRYEFVGGTMDKSLTFSGTN
                     GYFSIGAKRANGVLTFGAASKDCLCELEAAWTNNAGQNAVVLGGTNNVFVLKDGTEYY
                     TSGDGSVISFAEKPNGRLVISNATYTMNGRVCVGSGNVDAFNWTNSPNAAIEFRGTNP
                     LMRCVSTPNWKAWVLGTASGQPLLDPVHFKFVIPEQGYAAAPMQNQVANRTATLYGNT
                     VFEIDGSAYKGTEPVPLFYTAGGWLDMSQTVVDQLNASAILPPSWTLVWDKGKTIYAK
                     PPPSGSVLTIR"
     gene            1315..3687
                     /locus_tag="PK576_10035"
     CDS             1315..3687
                     /locus_tag="PK576_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61969.1"
                     /translation="MKTRWMLLAVIWAAGTLSANDALRLSDDLCSLRFADGRLVIADK
                     TGTDILAVGKVPFLWSVPCAVPKSVQRAANGRLRIAYEITNASGTNFPFSAEAYLTGH
                     GFAVDFVSEIPPCFRSGGQMVEVFRLNDTGRSQQVTKCGYWQRCKPAVAEGWYDPGVP
                     FEVKSMNVKPYRAKSGQVFWCRNTGWSGARTECPGFPRTSTNGVYHGRLEFVTGVDSE
                     DAQCAAAQVDGRPFVIALATERPFNLFESGAPKFRLTVKPLIDGWRTVRTVARDFDGK
                     VVFDQSERFEFKIGVPFVRDYALPAVEEGARGIWFIESTVGEGEREDGFARMNVAVLP
                     PHRFTHRDTSIAAMASYPETPEAERLMERLGVSILRYGDNNHFRTNYGITAYASRHAP
                     PEMFDPQNPKHVKIIEKEIVGRIKERGNPYFEFGNEVGWHKTHEEQTRLVKCYVSWLK
                     VIRARLAAEGLGHVKIITFGIQPDYSAHMMRIMKDEGVFDLVDGLTLHPGRGYYTADV
                     THGGWVYRGIIQRARARFAELGYPDKEIHMTECYAATHPNDGWKDSLRQATENTLLSL
                     VIASVEPNVKNMMFYKLHQGTSQDPHGYPLAHPSGQTTVNAEYDFGLLMRDDSPKPSL
                     LSYAATCEHLDGAKFLGEKGAGTNRTDTLRAFVFDTPRGRLAVLFDRNEGGMLYKLWQ
                     RELPKGVYRDALFRHKDPWVRHWHRTKPYAFKTAGAVRVFDMIGRERPAAVKDGKFEI
                     ALDGEPVFVYGLDPVDILPAASFPAAPAVGREPSEDEYAAEEKSSGKVSD"
BASE COUNT          795 a         1135 c         1346 g          784 t
ORIGIN      
        1 gaccttttac atgaatgtgc cgcaggcgac accccaatat acggggcggc ggtattttga
       61 atggcggtcc gggtattcgg tttccaatga gatggtcgtt tccgggtcaa gtgttgccag
      121 cggcttctcg acctttgaca tgaacggcgc ctgggacctt ctccggattc aggacacggc
      181 caaggcctcg cctccggtgg tgatgaatgg cgtctcgaac cgggttgaag tgctgggggg
      241 cgagctgtcg tcgggaagct gccaggtcaa tggccggtac aatcggttgc tcctgtcgga
      301 cggtctgttg gtgacgggaa acaagggaac attgacaatg ggaggaaatg ggccgaacct
      361 gatccgtcag acgggcggta cctgctcggc gcccgacggc gtgaccatcg ggggcttttc
      421 caatcgctac gagttcgtgg gcggcacgat ggacaagagc ctgaccttca gcggcacgaa
      481 cggatacttt tccatcgggg cgaagcgtgc gaacggggtg ttgacattcg gtgcagcttc
      541 taaggactgc ctttgtgaac tggaggccgc ctggacgaac aacgccggcc agaatgcggt
      601 cgtccttggc ggcacgaaca acgtttttgt cctgaaggac ggcacggagt actatacgtc
      661 aggtgacggg agcgtgattt cctttgcaga gaagccgaac ggccgattgg tcatttcgaa
      721 tgcgacctac acgatgaacg ggcgcgtctg cgtcggcagc ggcaatgtgg atgccttcaa
      781 ctggacgaac agcccgaacg ccgcgattga attccgtggc acaaacccgc tgatgcgttg
      841 tgtctccacg ccgaattgga aggcctgggt cctcggtacg gcgagcggac agccgcttct
      901 ggatcccgtg cacttcaagt tcgtcattcc cgaacagggc tatgctgccg cgccgatgca
      961 gaaccaggtc gcgaatcgaa cggcaacgct ttatggtaat accgtatttg aaattgatgg
     1021 ttccgcttac aaggggacag agccggtgcc gctcttctac acggcgggag ggtggctgga
     1081 tatgtcccaa accgtcgttg accagctcaa tgcaagcgcc atcctgcccc cgagctggac
     1141 ccttgtctgg gacaagggga agacgatcta cgcgaaaccg ccgccgagcg ggagtgtgct
     1201 gacgatccgt tgatgcaggg gctccagcga atgaggaggc tatacgctat cgggagacgt
     1261 gaccttcggc gcgacgacct ttccggtcgg cttccgttga gggggtgccg ggcgatgaag
     1321 acgaggtgga tgctgctggc ggttatctgg gctgcgggga cgctttcggc gaatgacgcg
     1381 ctgcggcttt cggatgatct gtgttcactg cgctttgccg acggccgact ggttattgcg
     1441 gacaagacgg gaaccgacat ccttgcggtc gggaaggttc cgttcttgtg gtcggtgccg
     1501 tgcgctgtgc cgaagtcggt tcagcgggcg gcgaacggcc gtctgcggat cgcctacgag
     1561 atcacaaatg ccagcggcac gaactttccg ttttcggcgg aagcgtacct gacgggacat
     1621 ggtttcgccg tggatttcgt ctcggaaatc ccgccgtgct tcaggagcgg cggacagatg
     1681 gtcgaggttt tccgcctgaa tgacacgggg cggagccagc aagtgacgaa gtgcggctat
     1741 tggcaacgtt gcaagccggc ggtggcggaa gggtggtatg atcccggggt gccgttcgag
     1801 gtcaagagca tgaacgttaa gccgtatcgc gcgaagtccg gtcaggtctt ctggtgccgg
     1861 aacaccggct ggtcgggcgc gcggacggag tgtccggggt tcccccgcac gtcaacgaac
     1921 ggcgtctatc acggacggct tgagttcgtg acgggcgtcg actccgagga tgcgcaatgc
     1981 gccgcggcac aggtggacgg gcggccgttt gtgatcgcgc ttgcgacgga gcgcccgttc
     2041 aacctgttcg agagcggtgc gccgaagttc cggcttacgg tcaaaccgct aatcgatggc
     2101 tggcggacag tacggacggt ggcgcgggac ttcgacggca aggtcgtttt cgatcagtcc
     2161 gagaggttcg agttcaaaat cggcgtgccg ttcgtcaggg actatgcatt gccggccgtg
     2221 gaagagggcg cacgcggcat ctggttcatc gagtcgacgg tcggggaggg cgagcgcgag
     2281 gatggcttcg cgcggatgaa tgttgccgtg ttgccgccgc accggttcac gcatcgcgac
     2341 acgtcgatcg cggcgatggc gtcctatccc gaaacgcccg aagccgagcg gctgatggaa
     2401 cgtctcggcg tgtcgatcct ccgctacggc gacaacaacc atttccggac aaactatggc
     2461 atcaccgcct acgcctcccg tcacgcgccg ccggagatgt tcgatccgca gaatccgaag
     2521 cacgtcaaga tcatcgagaa ggagatcgtc ggccgcatca aggagcgggg gaacccttat
     2581 ttcgaattcg gcaacgaagt cggttggcac aagacgcacg aggaacagac gcggcttgtc
     2641 aaatgctacg tgagttggct caaggtcatc cgtgcccggc tcgcggcgga aggactcggg
     2701 cacgtcaaga tcatcacctt cggcatccag ccggactatt ccgcgcacat gatgcggatc
     2761 atgaaggatg agggggtctt tgacctcgtg gacgggctga cgcttcaccc cgggcgcggc
     2821 tattatacgg ccgatgtgac gcacggcggt tgggtatacc gcggcattat ccagcgtgcc
     2881 cgcgcccgct tcgccgagct cggttaccca gacaaggaaa tccacatgac cgaatgctat
     2941 gcggcgacgc atccgaacga cggctggaag gattcgctgc gccaggcgac cgagaacacg
     3001 ctcctgtcgc tcgtcatcgc atccgtcgag ccgaacgtca agaacatgat gttctacaag
     3061 cttcaccaag gcacgagcca agatccgcac ggctatccgc tcgcgcaccc gagcgggcag
     3121 acgacggtga acgccgagta cgatttcggg cttctgatgc gcgacgactc gcctaagccg
     3181 tcccttctca gctatgcggc gacatgcgag catctggacg gcgcgaagtt tctcggtgag
     3241 aagggagccg gcacaaaccg cacggatacc ctgcgcgcat tcgtcttcga cacgccccgc
     3301 gggcggctgg cggtcctctt cgaccggaac gaaggcggca tgctgtacaa actttggcag
     3361 cgcgaactgc ccaagggcgt ctaccgcgac gcgcttttcc ggcacaagga tccgtgggtg
     3421 cggcactggc accgcacgaa gccctatgcg ttcaaaacag cgggcgcggt gcgcgtcttc
     3481 gacatgatcg gccgcgaacg gccggcggcg gtcaaggacg ggaagttcga gatcgcgctc
     3541 gacggcgaac ccgtctttgt ctacggtctg gatccggttg acatcctgcc cgccgcgtcg
     3601 ttccctgcgg ctccggccgt cggacgggag ccgagcgaag atgaatacgc cgccgaagag
     3661 aagtcgagcg gcaaggtgag cgattgatcg ggtaattccg ttttgaaacg ggcgaaacga
     3721 cgggctatcg gtccgtgaca gggctgtgcg aaggtcatgc gtttctggtg aaaaggggag
     3781 cagtgagaag ggagcgtggg cgtcctcgcc cgcaacaacg tgtcttgcgg ctgctggttt
     3841 tatccgcaga tttgcagatt gcgcagattt tttgttttgc cgcaggatgg gagggacgag
     3901 cgtctgctcg tccgggcggc gtttcacgcc gccgcttttg aacgccgaac gcccgacgcc
     3961 gaactcagaa tgtcgaagtg aaaaaactcc taactccttg cctacaaatt gacaggatga
     4021 caggattttc aggattgaac aacatcatgt taatcatgga
//
LOCUS       DAOSLE010000199         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19464_length_4059_cov_5.732517, whole genome shotgun sequence.
ACCESSION   DAOSLE010000199 DAOSLE010000000
VERSION     DAOSLE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19464_length_4059_cov_5.732517"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..349)
                     /locus_tag="PK576_10040"
     CDS             complement(194..349)
                     /locus_tag="PK576_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61970.1"
                     /translation="MIDPDSDSDPDPDPDLDETLKPRNPETRNPETLKPDSDSDPDPD
                     LDETLKP"
     gene            630..1310
                     /locus_tag="PK576_10045"
     CDS             630..1310
                     /locus_tag="PK576_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61971.1"
                     /translation="MSHSQAAQLPRGLIVEMDFAVLPGHALMQDICRERLAAEGVAFD
                     VKRAAKAFCGRSFTSGLNALCVKAQKTVDAAALAADCNARFEEALSAALSEMPSGFVA
                     FVKAVLAKGVKVVLMTRADVEAVRARFGDSDPEKLIVSHETMPGFCCCGWEGWRRVAR
                     KHDLHERLCVAVAGSGLSVKGALTSGLGVMAKLHPLVEHQDISGSDALITEYSAGLVS
                     EVCRILRI"
     gene            1398..2201
                     /gene="mazG"
                     /locus_tag="PK576_10050"
     CDS             1398..2201
                     /gene="mazG"
                     /locus_tag="PK576_10050"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOE61972.1"
                     /translation="MERTFSGIARLDEVMRRLRASDGCPWDREQTLKTLKPCLLEETY
                     ELLEAMDNGDPEHHREELGDVLLQVVFQCAIREEEGCFTFDEVAQVLADKLIRRHPHV
                     FGEVRVDSSSQVLRNWEAIKLTEPDKPANRSALDGVPRSLPALLKAQRMQAKAARVGF
                     DWANAAGAMTKVGEELAELQEAATSGDLRRVTEEMGDLLFSLVNTCRFLDVDAEQALE
                     TANRKFERRFREVERRLRAQGRMLKDCTLEELDAVWEVVKEEETCNDPV"
     gene            2355..2600
                     /locus_tag="PK576_10055"
     CDS             2355..2600
                     /locus_tag="PK576_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012194023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE61973.1"
                     /translation="MSDEEKNANPQGRPRPVIDADECKSCGRCVNACPRKCLRLAADR
                     LNRRGVKPAEYTGEGCTGCAICFYNCPEPYAIRIEKP"
     gene            2650..3705
                     /gene="vorB"
                     /locus_tag="PK576_10060"
     CDS             2650..3705
                     /gene="vorB"
                     /locus_tag="PK576_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOE61974.1"
                     /translation="MTRFVKGNEAVVIGALYAGCDVYFGYPITPASEIAHAAAKVFPS
                     VGREFLQAECETASINMVYGAAAAGTLAMTATSGPGMSLMQEGLSYMAGANLPGVVVN
                     VMRAGPGLGNVYPEQGDYNQAVKGGGHGNYACAVVAPASVQEMCDLTMRAFGLAFAYR
                     TPAIVLADGLLGQMMEALVLPERESPRPDTSDWALHGTAATRSHLVTSIYLDAAAQEA
                     HNLRLQAKYAAMRSEARHETYLCDDAELVLLAYGICSRICRTAAEMLRAEGIRAGVFR
                     PITLNPFPAEALCAVLEGRRGMVVELSAGQFRDDVLLHLAKEGGRTQPITLCNRLGGM
                     VMTVEDVVQAARAVAKR"
     gene            3715..>4059
                     /locus_tag="PK576_10065"
     CDS             3715..>4059
                     /locus_tag="PK576_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoisovalerate oxidoreductase"
                     /protein_id="HOE61975.1"
                     /translation="MTEKKTVGMYETFVRSGKETRSTHYCAGCGHGIIHKLITEALTE
                     LGIQDRTIIVNPIGCAVFGYYYWDCGNVGAAHGRAPSVGTAINRVRRNAVVISYQGDG
                     DLGAIGFNNTFQA"
BASE COUNT          767 a         1071 c         1415 g          806 t
ORIGIN      
        1 agctcgtaac cggttgagtt ggcggggacg cccgctccaa ctctctttgg tttcagggtt
       61 tcatccaaat cggggtcggg gtcgggatcg ctatcgggtt tcatggtttc aaggtttctg
      121 gtttcaaggt ttcatccaaa tcggggtcgg gatcgggatc gctatcgcta tcgggtttca
      181 gggtttcaag gtttcagggt ttcagggttt catccaaatc gggatcggga tcgctatcgc
      241 tatcgggttt cagggtttca gggtttctgg tttctgggtt tcgtggtttc agggtttcat
      301 ccaaatcggg gtcgggatcg ggatcgctat cgctatcggg atcgatcatt taaccacgga
      361 ataccaccgc ctcaccgcct cacagtctca cagcctttga gattgcgggc tttgcccgca
      421 tctcaatacg gtgcggcggg gaccgcgaca aaacccaatg tttacggggc tttgctagaa
      481 ttctacccgt tcaactgttc cttttgggtt taggggacac gcccggaatt gctgtggcgc
      541 agaaccggca cttgaccgca agtgagggga acgggtacac taaacggcat tttgatgaat
      601 caagaggcaa aaagtgagga gtcgagaaga tgagtcattc ccaagccgct caattgccgc
      661 gcggtctgat tgtcgaaatg gattttgcgg tgcttcccgg ccatgcgctg atgcaggata
      721 tctgccgcga gcgattggcc gcagagggtg tcgcgtttga tgtcaagcgc gcggctaaag
      781 cattctgcgg cagatcgttc acctccgggt tgaacgcgct gtgcgtcaag gcgcagaaaa
      841 cagtggacgc ggcagcactg gcagcagact gcaacgcacg gtttgaagag gcgctgtccg
      901 ccgctctgtc ggagatgccg tccggattcg tggcgttcgt caaggcggta ctggcgaaag
      961 gggtgaaggt ggtcctgatg acgcgggccg acgtcgaggc ggtgcgggcg cggttcggcg
     1021 acagtgatcc ggagaagttg atcgtttcgc acgagacgat gccgggcttc tgctgttgcg
     1081 gatgggaagg ctggcgtcgc gtggcgcgca agcacgatct gcatgagcgg ctgtgcgtgg
     1141 ccgttgcggg gagcgggctc tcggtcaagg gcgcgctgac ctcgggattg ggcgtaatgg
     1201 ccaaactcca tccgctggtc gagcatcaag acatcagcgg cagtgatgcg ctgatcaccg
     1261 aatattcggc cggactggtc tccgaggtct gccggattct gcgcatctga agtgcgtgac
     1321 agtgcggagc gctgtcagat cgagagagcc ccgtttggat gccgaacggg gccatgtctt
     1381 ttttccgggg aggaagtatg gagcggacat tcagcgggat cgcccgatta gatgaggtga
     1441 tgcgccggtt gcgcgcgtcc gacggatgcc cgtgggatcg tgagcagacg ctgaagacgc
     1501 tgaagccctg cctgcttgaa gagacctacg aactgctgga ggcgatggac aacggagatc
     1561 ccgagcacca tcgcgaggag ttgggcgatg tgctgttgca ggtggtgttc cagtgcgcga
     1621 tccgggagga ggagggatgc ttcaccttcg acgaggtggc gcaggtcttg gccgacaaac
     1681 tgatccggcg gcacccgcat gtgtttggtg aggtacgggt cgattcgtcc agtcaggtcc
     1741 tgcggaactg ggaggcgatc aagctgaccg agccggacaa gccggcaaac cgctccgcgc
     1801 tggacggggt gccccggagt ttaccggccc tgctcaaggc gcagcgcatg caggccaaag
     1861 ccgcgcgtgt gggttttgac tgggcaaacg ccgcgggcgc gatgaccaag gttggggaag
     1921 agctggccga gttgcaggag gccgccacct ccggtgatct gcggcgcgtc acagaggaga
     1981 tgggtgatct gctgttctcg ctggtcaaca cctgtcgttt tttggatgtg gacgccgaac
     2041 aggcgttgga gaccgcgaac cgcaaatttg aacggcgttt ccgcgaagtc gagcggcgtc
     2101 tccgtgcgca aggccggatg ctcaaggact gcacactgga ggaacttgat gccgtgtggg
     2161 aggtcgtcaa ggaggaggaa acgtgcaacg atccggttta ggcaagcggc attccgcagg
     2221 gcgcaaggga gtcgcggatc gttttcgcgc gaccttgcgc cagccagaca aacatggcac
     2281 actacatttt tcgtgcggaa ccggggcggc tgaaccctca aggggacgac acgggcgaag
     2341 gatgaggaga cgatatgtct gatgaggaga agaacgccaa tccgcaaggc aggccgcgtc
     2401 ccgtaatcga cgcggacgag tgcaaaagtt gcggacggtg tgtgaatgcg tgcccccgga
     2461 aatgcctgcg gcttgccgcg gatcggctca accgtcgcgg cgtgaagcct gcggaataca
     2521 cgggcgaggg gtgcacgggg tgcgccatct gtttttacaa ctgtccggag ccgtatgcga
     2581 tccggatcga gaaaccttga acaacgtgag cggacggggc tgcggggccc gtcccgggag
     2641 tgaaccagca tgacccgatt tgtgaaaggc aacgaggcgg tggtgatcgg cgccctgtat
     2701 gccgggtgcg atgtctattt cggctatccg attacgccgg ccagcgagat cgcgcacgcc
     2761 gcggcgaagg tcttcccttc ggtggggcgg gagtttctgc aggccgagtg cgagaccgcc
     2821 tcgatcaaca tggtctacgg cgcggcggcg gcgggcacgc tggccatgac cgcgacatcg
     2881 ggtcccggca tgagcctgat gcaggagggg ctctcctata tggcgggcgc gaatcttccc
     2941 ggcgtggtcg tcaacgtgat gcgcgctggc ccggggttgg gtaacgtcta tcccgaacag
     3001 ggggactaca atcaggcagt caaaggcggc ggtcatggca attacgcctg cgcggtggtg
     3061 gccccggcga gcgtgcagga gatgtgcgac ctgactatgc gcgcgtttgg gttggccttc
     3121 gcctaccgta cgccagcgat tgtgctggcg gatgggctgt tgggccaaat gatggaggcg
     3181 ctggttctgc cggaacgcga atcaccgcgg ccggacacct ctgattgggc gttgcacggc
     3241 accgccgcga cgcgttcaca tctcgtcacc tcgatttatt tggacgccgc cgcacaggag
     3301 gcgcacaatc tgcgtttgca ggccaaatat gcggcgatgc gttcggaggc gcgtcatgaa
     3361 acctacctgt gcgacgatgc cgaactggtg ctgctcgctt acgggatttg cagccggatc
     3421 tgtcggaccg ccgctgaaat gctgcgtgca gaaggaatcc gcgcgggcgt gttccgcccc
     3481 atcacgctga acccgtttcc ggccgaggcg ctttgcgccg tgctcgaagg acgccggggc
     3541 atggtcgtcg aactgagcgc gggacagttt cgggatgatg tgctgctgca ccttgccaaa
     3601 gaaggcgggc gcactcagcc catcacgctt tgtaaccgct tgggcggcat ggtgatgaca
     3661 gtggaagatg tggtgcaagc cgcgcgcgcg gtcgcgaaac gatagggttg gcgcatgaca
     3721 gaaaagaaga cagtcggaat gtacgaaacc tttgtccgta gtggcaaaga gacgcggtcg
     3781 acgcactatt gcgccggatg cgggcatggg attatccaca aactgatcac cgaggcgctg
     3841 accgaactcg ggattcaaga ccgcacgatc atcgtgaacc cgatcgggtg cgcggtgttc
     3901 ggatattact actgggattg cggcaacgtc ggggcggcgc acgggcgcgc gccgtcggtt
     3961 ggcacggcga ttaaccgtgt gcggcgcaat gcggtggtca tctcgtacca aggggacggc
     4021 gatttgggcg ccattggttt caacaacacg tttcaggcg
//
LOCUS       DAOSLE010000200         3982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_19987_length_3982_cov_7.291062, whole genome shotgun sequence.
ACCESSION   DAOSLE010000200 DAOSLE010000000
VERSION     DAOSLE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19987_length_3982_cov_7.291062"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..490
                     /locus_tag="PK576_10070"
                     /pseudo
     CDS             242..490
                     /locus_tag="PK576_10070"
                     /inference="COORDINATES: protein motif:HMM:NF025041.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(529..605)
                     /locus_tag="PK576_10075"
     tRNA            complement(529..605)
                     /locus_tag="PK576_10075"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(569..571),aa:Ile,seq:cat)
     gene            666..1637
                     /locus_tag="PK576_10080"
     CDS             666..1637
                     /locus_tag="PK576_10080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE61976.1"
                     /translation="MTREIASIIRANEAGRALQDFLADRFTYHTADEWMCRIAEGRVR
                     VNGHTATPDRILSVGDHLRYDASDIPEPPVDCTFRILIDDPLLLVIDKPGNLPCHPGG
                     RYFRHTLWALLQTQCGLAHPVFVNRIDRETSGLVLIAKTPEAGRNLARQFAGGRVTKT
                     YTVLVEGMFPERVEARGWLHPDPDSAVRKKRRFSPAPAGSAAPGDAAEWSETVFERER
                     VCDGFSVVTARPRTGRLHQIRATLLSLGFPVVGDKLYGVDEQCFLRFCSGGLTERDRA
                     RLRMGRQALHAVRLAFRHPHFGTPTDVDAPLPSDMEALIGGHAQACL"
     gene            complement(1775..3763)
                     /locus_tag="PK576_10085"
     CDS             complement(1775..3763)
                     /locus_tag="PK576_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61977.1"
                     /translation="MKSYVVSVVAVLAVLAVRAEDRTWTGAVDTDWNTAANWTDEAVP
                     GTSDNALLTNNAGTIQVWVTHSPETAVGSLTVSNVNASGHTTTLTITNAAFGVQAGSV
                     LFGVNSVLDIRDGGAVSYLGEASAAPFFNVRDGGEIRINNGGGLLLTNLYSATGGLNR
                     YMYVGDGSAGTIRIGDGGTFVMGASSAGVATNTLMRLGYNNGGNGLLEMTGDSTFVMP
                     EGNKDTSALYVGSGAGASGTVFLADNAKCLVSNVLWGGSANATGIVSLSGNALLRPFM
                     RARQYGFGNGANAYGELTLHDNAWFDGAGTADQHFYCGKDGGTGRLNVYGGTLQIRNF
                     FHIGFNGTGYFTLTNGLVDAMGQYGRGLTFGVANGNGKVVYANALISGGTLDIKTPPD
                     IYAVEAYSGLQVGKTVAKTVTTTSRVFSEMVQSGGVITNRAYLFIGQGMNATGTVVQT
                     GGSFINGTSDKDSERAFIGSFGGCGSYVLSNGTFSTLKDVYVGGFPDALWPPSLPNAE
                     STNHWNKYADFYTNKVSTGVLRIDGGSFAASNATIHVGAQGVGTLVIGKDAVCIARDI
                     VLDTNTSSTVRLEFGPDGIGTLCATNSLTVCAGAKLEVDATAYPAATTWTKLIDCTSR
                     TGAFAEADISVIGEGVVRQDRDEDIWLYRVRGTMISVF"
BASE COUNT          810 a         1287 c         1116 g          769 t
ORIGIN      
        1 ctgtgaggcg gttgtgttct gtggtgaaac aatcgctccc gatcccgatc ccgatagcga
       61 tcccgacccc gatgaaaccc tgaaaccatg aaacccgaaa ccctgaaaca atcgatcccg
      121 atcccgatag cgaccccgac cccgatgaaa ccatgaaacc atgaaccccg aaaccctgat
      181 accctgaaac cagaaacccc ggcatggatc gccgccgcgc agaagatcga ccagcacgta
      241 cgacgacgct tactatgccg acgagacgct acccccgatt agcttgacgc tccccaaggt
      301 ccaacaggaa catcaacgcg gacatctctt tccgtttttt gccaacatgt ttagcgaggg
      361 cgcgaaccgg aacgtgcaga gccgtcaccg gcaacggcca gggaaacaaa gacacgtccc
      421 ctttctccaa agacgcgggt caaccattgc gcgccggtgc ggcaaagtgg taacgtaccc
      481 ctgttcttga tccggatgaa tgccgtgatg ccagattgcg tcggtggatg gtgggcccag
      541 cagggctcga acctgcgacc aaaggattat gagtcctccg ctctgaccga ctgagctatg
      601 ggcccgtgac aaccgcgtca ctatatcacg ggttcacgat gcgcttcaac gccaaagggg
      661 tagagatgac acgcgagatc gcctccatca tccgcgcaaa cgaagccgga cgcgcattgc
      721 aagactttct ggcggaccgg ttcacgtacc acaccgcgga tgaatggatg tgccgcatcg
      781 cggaggggcg cgtgcgggtg aacggccaca ccgcaactcc ggaccgaatc ctttccgttg
      841 gggatcatct gcgttacgac gcctccgata tccccgagcc gccggtggat tgcacttttc
      901 ggattctcat cgacgatccg ttgctgctgg tgatcgacaa acccggcaat ttgccgtgcc
      961 accccggcgg acgctatttc cggcacacgc tttgggctct gcttcaaacg caatgcggcc
     1021 tcgcgcatcc ggttttcgtg aatcggattg accgcgagac ctccgggttg gtgctgatcg
     1081 ccaaaacccc ggaggcgggc cggaaccttg cgcggcaatt cgccgggggc cgggtcacga
     1141 aaacctacac tgttctggtg gagggaatgt tcccggagcg ggtagaggcc cggggctggc
     1201 tgcatcctga tccggattct gcggttcgca agaaacggcg tttctcgccg gccccggcag
     1261 gttcagccgc gcccggtgat gcggccgaat ggtcggagac cgtgtttgag cgtgaacgcg
     1321 tgtgcgacgg gttcagcgtg gtgactgccc ggccccgcac ggggcgtctc catcagatcc
     1381 gcgcgacgct gctgtcactc ggtttccccg tggtcggcga caaactgtac ggggtggacg
     1441 agcaatgctt tctccgtttc tgctcgggcg ggctcacgga aagggaccgg gcgcggctcc
     1501 gcatggggcg tcaggcgctc cacgcggtcc gcctcgcatt ccgtcacccg catttcggca
     1561 cgccgaccga tgtggacgcc ccgctccctt cggacatgga ggccctgatc ggcggccatg
     1621 cgcaggcttg cctgtaactt gccaggatga caggattttc aggattgaac agcatcatgt
     1681 taatcatgcc ctaaacgtat tccagggttt ttcgcggcga agccgcgcgg caggctaatt
     1741 aactaattaa actgggttgc gggcttcgcc cgcattagaa gacgctgatc attgtcccgc
     1801 gcacccggta gagccagatg tcctcatcac gatcctgccg caccacaccc tcgccgatca
     1861 ccgagatgtc tgcctcggca aacgcacccg ttcgcgatgt gcaatcaatc agctttgtcc
     1921 atgtggtcgc ggccggataa gcggtggcgt ccacctccaa cttggcaccc gcgcacacgg
     1981 tcagcgagtt ggtcgcgcac agcgtaccga taccgtcagg tccgaactcg agccggaccg
     2041 tcgaactcgt gtttgtgtcc agcacgatat ccctcgcgat gcagaccgcg tccttgccga
     2101 tcaccagcgt cccgacaccc tgcgcaccca catggatcgt cgcgttactg gctgcaaagc
     2161 ttcccccgtc gatcctcaag accccggtgc tcaccttgtt ggtgtagaag tccgcgtatt
     2221 tgttccagtg gtttgtggat tcggcattgg ggagtgaggg gggccacagc gcatccggga
     2281 acccgcccac gtagacgtcc ttcagcgtgc tgaacgtacc gttcgacagc acgtacgagc
     2341 cgcatccgcc gaaggaaccg ataaaggcgc gctcactgtc tttgtcgctc gtgccgttta
     2401 taaacgaacc gccggtctgg acgaccgtgc cggtcgcgtt cattccctga ccgataaaga
     2461 gataggcgcg gtttgtgatc acgccgccgc tctgaaccat ctcgctgaaa acacgcgagg
     2521 tcgtggtcac ggtcttggcc acggtcttcc cgacctgcaa gcctgagtag gcttcgaccg
     2581 catagatgtc tggcggggtc ttgatgtcca gcgttccccc cgagatcagc gcgttcgcgt
     2641 acacgacttt cccgttgcca ttggccacac cgaaggtgag gccccgccca tattggccca
     2701 ttgcatcgac cagaccgttg gtgagggtga agtatcctgt cccgttgaaa ccgatatgga
     2761 aaaagttcct gatctgaagt gtgccgccgt aaacattcaa tctcccggtc ccgccgtctt
     2821 tcccgcaata gaagtgctgg tctgccgtcc ctgcgccgtc aaaccaggcg ttgtcatgga
     2881 gcgtcagttc gccgtacgca ttcgctccat tcccgaagcc gtactgacgg gctctcatga
     2941 atggccggag cagggcgttt ccggacagcg aaacgatccc ggtcgcattt gccgaaccgc
     3001 cccacagcac gttggagacc agacacttcg cgttatccgc cagaaaaacg gtgcctgatg
     3061 cgcccgcccc ggacccgacg taaagcgcgc tcgtatcttt attgccctca ggcatcacaa
     3121 acgtgctgtc gcccgtcatc tccagcaacc cgttcccgcc attgttgtaa ccgagccgca
     3181 tgagcgtgtt ggtggcgacc ccggccgatg acgcgcccat cacgaacgtc ccgccgtcgc
     3241 cgatccggat ggtccctgcg gaaccgtccc cgacatacat gtaacggttc aatccgcccg
     3301 tggcggaata gaggttggtg agcagcagac cgcctccgtt gttgattcgg atctcacccc
     3361 cgtcccggac attaaagaac ggcgcggccg aggcctcccc gaggtaactc acggccccgc
     3421 cgtcccggat atcgagcaca ctgttcacgc cgaacaacac cgagccggcc tgcacgccga
     3481 aagccgcgtt cgtgattgtc agggtggtgg tatggcctga tgcgttgacg ttcgagaccg
     3541 tcaaggaacc gaccgccgtc tcaggcgaat gggtcaccca gacctgaatc gtccccgcgt
     3601 tattggtcag cagcgcgtta tccgacgttc ccggcaccgc ctcatccgtc cagtttgcag
     3661 ccgtattcca gtccgtatca accgcccccg tccacgtccg gtcctccgcc ctgaccgcca
     3721 aaaccgccaa aaccgccact actgacacaa catacgattt catagcccgt ttccttccgt
     3781 tctgacaaac caacgcccta tatcacaacc attcatccgg gtagaccgga aacaaaccgg
     3841 ctcccatgct tgatatctac tatgtgggga ggggcgaaaa gagtcaactc taaaaatccg
     3901 atcgccagcg gcacaaaaag aagaagggtc gaggccgggg gagaacaaga caggattaac
     3961 aggatttaca ggattggaga tg
//
LOCUS       DAOSLE010000201         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20173_length_3951_cov_7.810062, whole genome shotgun sequence.
ACCESSION   DAOSLE010000201 DAOSLE010000000
VERSION     DAOSLE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20173_length_3951_cov_7.810062"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..82)
                     /locus_tag="PK576_10090"
     tRNA            complement(6..82)
                     /locus_tag="PK576_10090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Pro,seq:ggg)
     gene            complement(261..1886)
                     /locus_tag="PK576_10095"
     CDS             complement(261..1886)
                     /locus_tag="PK576_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61978.1"
                     /translation="MKKTWTAMLILGLIGLRSGATILYRMTFDHTGAAGGEANAIYTA
                     GPGDLLDPHQSVIRYGTNPVPQIVASDGPQGGCALVTATQLPGSGQGPGYTAATTNSK
                     LYFVHGGHDRGLTLEAVFRATAWTANGWGGMVNYNNQHFLGFAWGTKNNPHIKFAVNN
                     IGVITNVPAESLCDDAWHHVAGVWTRDADGVTGRLEIFLDGVSVSSHAVPIPASAITI
                     PTGGWGFSAEGYTTPPRLRSFIGELDAAAVADDVRGPGRFLVAGCDADVFPDLYTEDF
                     ATGWSNGVAGFMGSGAAVPGRWTLRDGELSKWQVMETGTPQGLALAFTNGSFNSSWWP
                     TGMQTPFRFPVTGPVTVEFSARMRIACNAVSGKIWDARIHLMTRDQNGYGLSLKRSLD
                     SVAHGADNKVYVRIIKYRGCTVALGTGSPSDWAEGDGASATDEVMVADADETGFIRLH
                     VRLTQEEPNGPVTVRLWHTESGVADTSLQNPDLTWMDDGSRFGAILNLRELNWLGVNA
                     EAQGSPNGPDAGVWFDDLRVERIRRSGMVMEIR"
     gene            2317..>3951
                     /gene="carB"
                     /locus_tag="PK576_10100"
     CDS             2317..>3951
                     /gene="carB"
                     /locus_tag="PK576_10100"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOE61979.1"
                     /translation="MLRNDIHKVLIIGSGPIIIGQACEFDYSGTQACKALRECGYKIV
                     LVNSNPATIMTDPVMADATYIEPLNAERIEQIIAQERPDALLPNLGGQTGLNLAMELS
                     KKGILDKYGVRVIGVNLDAIERGEDRQIFKDTMTKLGIETPQSEIAYSVENAETIASR
                     LGYPVVVRPAYTMGGTGGGLVYNVEELRAIVIRGLEASLTHQCLIEESVLGWEELEVE
                     VVRDAKNQMIAVCFIENIDPMGVHTGDSFCSAPMLTLSDEVRQRLQEYAFRIVESIGV
                     IGGTNVQFAHDPATGRIVIIEINPRTSRSSALASKATGFPIALISAKLASGMTLDEIP
                     YWRDGTLEKYTPSGDYIVLKFARWAFEKFRGVEDKLGTQMRAVGEVMSIGKTYKETFQ
                     KAIRALENGRHGLGFAKDFNRKTLPELLDLLRFPNSERQFILYEALRKGATDEQLFGL
                     TKIKPYFLGQMRELVELEERILQHKGGLPPDDLLIQAKRDGFSDKYLAKILGVLESVI
                     RTRRTALGIVETWHAVPVSGVENAYYYYSSYNGVRNE"
BASE COUNT          893 a         1225 c         1087 g          746 t
ORIGIN      
        1 aaaaatggtc ggggcgtcgg gatttgaacc cgagaccttt tgcaccccaa gcaaacgcgc
       61 taccaggctg cgctacgccc cgaaaaatac aaagtgcaga gcagactgta ccaagtcgga
      121 agacggcacg tcaaacggaa tcgcccggaa acgaaaaacc gcaacaccga accgatacgc
      181 cgcacagcac gaaacgtcat cggatcgcac ccagacggac gcgccgcagc cgcatcgtgc
      241 caacggctca ccgaaaagag tcaccggatt tccatcacca tgccggaacg ccggattcgt
      301 tccacccgca ggtcgtcaaa ccaaacgccc gcatcggggc cattcggtga accctgcgcc
      361 tcggcattga cgccaagcca gttaagttcg cggagattca gaatcgcgcc gaaacgcgat
      421 ccgtcatcca tccaagtcag gtcgggattt tgcagtgagg tgtccgccac tccactctcg
      481 gtatgccaga gccggacggt caccggtccg ttcggctcct cctgcgtcag acgcacatgc
      541 aggcggatga aacccgtctc gtcggcatct gccaccatga cctcatcggt ggcactcgca
      601 ccatcacctt cggcccagtc cgaaggcgat cccgtaccca atgccactgt acaccccctg
      661 tatttaatga tccggacgta gactttgttg tccgcgccat gcgcgacgga gtccagactc
      721 cgcttgaggg acaggccgta tccgttttgg tcacgggtca tcaaatggat tctggcatcc
      781 cagattttac cagacactgc attgcaggcg attcgcatgc gcgcactgaa ctccacggtc
      841 accggccctg tcaccgggaa ccggaagggc gtctgcattc ctgttggcca ccaactgctg
      901 ttaaaactcc cgtttgtgaa agcgagcgcc agcccttgtg gcgtaccggt ttccatcacc
      961 tgccacttgc tgagttcccc atcccgcaaa gtccacctgc ccggaacggc tgcgccactc
     1021 cccataaatc cggcgacccc gttagaccag ccagtcgcga aatcctcagt atagaggtcc
     1081 gggaacacgt ccgcatcgca ccccgcgacc aaaaaccgtc ccgggccgcg cacgtcatcc
     1141 gccactgccg cggcatccaa ctccccgatg aaagagcgca aacgcggcgg agtcgtgtat
     1201 ccctcggcgg aaaaacccca cccgccggtg ggaatggtga tggcagaagc gggaatcggc
     1261 accgcgtggc tcgagaccga aacaccgtcg agaaaaattt cgagccgccc ggtcacgccg
     1321 tccgcatccc gtgtccagac acccgccaca tggtgccatg cgtcatcgca cagagattcg
     1381 gccgggacgt tcgtaatgac accaatgtta ttcaccgcga acttgatgtg cggattattt
     1441 ttcgtgcccc aagcgaaacc gaggaaatgc tggttgttat agttcaccat gccgccccaa
     1501 ccattcgcgg tccaggctgt ggcccggaaa accgcctcca gagtcaaccc gcggtcgtgg
     1561 cccccatgaa caaagtaaag cttgctgttg gttgtggctg cggtgtatcc cggcccttgt
     1621 ccgcttcccg gaagctgcgt ggccgtgacc agcgcgcaac cgccctgcgg accgtcgctc
     1681 gcaacgattt gcggtacggg gttggtgcca tagcgtatga ccgactggtg cgggtcgagc
     1741 aaatcacccg gtcctgccgt gtaaatcgcg ttggcttctc cgcctgccgc acccgtgtga
     1801 tcgaaagtca tccgatagag aatggttgca ccggaacgta aaccgatcaa accgagaatc
     1861 aacatagccg tccaagtttt tttcatgaca ccctcccttt gcggttggaa acgccacttc
     1921 tccctcattg gagtaaaccg ggaacacatt gctttcagta atgaaagtca ttatgcaaag
     1981 gggcacgaaa cgagtcaatg tcgaaagtcc ggttttgggg ataacaacga acgcgatgaa
     2041 tctctttcgg tcatcaatca atagggacgg ttcggggacg ttcctactgg ttgcagcacg
     2101 tttagcgcaa caaacagccg ggttacagac gcgggagaag ggggttccga aaacaagaaa
     2161 ccccaaacca ggaacccgaa tccctcagcg ggcgagcttt gactcgacga gcgcgggcga
     2221 aactggcatc atcatgagcg tgttttgagg cggctccccg cgcgtcggcg cgggctggag
     2281 acgtccgaat caagtccgaa aggtcaggcc caccctatgc tgcgcaacga tatccacaag
     2341 gttcttatta tcggctctgg tccgatcatc atcggtcaag cgtgtgaatt cgattattct
     2401 ggcacccagg catgtaaggc gctacgcgaa tgcggataca aaatcgtcct ggtaaactcg
     2461 aacccggcca cgatcatgac ggacccggtg atggcggatg ccacctatat cgagcctctc
     2521 aacgccgaac ggatcgaaca gatcatcgcc caagaacgcc ctgacgccct cctgcccaat
     2581 ctcggtggcc agaccggcct gaacctggcc atggaacttt ccaagaaggg gattctggac
     2641 aaatacgggg tccgtgtcat cggcgtcaac ctcgacgcga tcgagcgggg cgaagaccgc
     2701 cagattttca aggacaccat gaccaaactg ggtattgaaa cgcctcagag cgagatcgcc
     2761 tattccgtcg agaacgccga gacgatcgcg tcgcgtctgg gttatcccgt ggtggtgcgc
     2821 ccggcataca ccatgggcgg taccggtggc gggctcgttt acaatgtcga ggaattgcgc
     2881 gccattgtca tccgcggtct ggaggcctcg ctgacccacc agtgcctgat cgaggagtcc
     2941 gtgctgggct gggaggagct tgaggtcgag gtggtccgtg acgcgaaaaa ccagatgatc
     3001 gccgtatgtt tcatcgagaa catcgatccc atgggggtcc acactggcga ctccttctgt
     3061 tccgcgccaa tgctgaccct ctccgacgag gtacggcaac ggctgcaaga gtacgctttc
     3121 cgcatcgtcg aatccatcgg tgtcatcggc ggcaccaatg tgcagtttgc gcatgatccc
     3181 gcaaccgggc gcatcgtgat cattgagatc aacccgcgta cttcgcgctc ctcggcactc
     3241 gcttccaaag ccaccggatt cccgatcgcc ctcatttccg ctaaactggc ctcaggcatg
     3301 actcttgacg aaatccccta ttggcgggac gggacccttg agaagtacac gccctccggc
     3361 gactacattg tgctgaaatt cgcccgctgg gcctttgaga agttccgcgg agtggaggac
     3421 aaactgggaa cccagatgcg tgcggttggc gaagtgatgt cgatcggtaa aacgtacaag
     3481 gaaaccttcc agaaggccat ccgcgccttg gagaacgggc ggcacggact cggtttcgct
     3541 aaggatttca acagaaaaac cttgccggag ttgctcgatc tgctgcgatt cccgaacagc
     3601 gaacgtcagt ttatcctgta cgaggcgtta cgcaaaggcg cgacggatga acaactcttc
     3661 ggcctcacga aaatcaagcc ttactttctc gggcagatgc gtgaactggt ggagctggaa
     3721 gaacggattc tccaacacaa aggaggcctg ccgccggacg acctgctcat ccaagccaag
     3781 cgggacgggt tttccgacaa atatctggcc aaaatcctcg gcgtcctcga gagcgtgatc
     3841 cggacccgtc ggaccgcgct cggaatcgtc gagacttggc acgccgttcc ggtcagcgga
     3901 gtcgaaaacg cctactacta ctactcgtcc tacaacggcg tgcgcaacga g
//
LOCUS       DAOSLE010000202         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20367_length_3921_cov_8.852302, whole genome shotgun sequence.
ACCESSION   DAOSLE010000202 DAOSLE010000000
VERSION     DAOSLE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_3921_cov_8.852302"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1651)
                     /locus_tag="PK576_10105"
     CDS             complement(266..1651)
                     /locus_tag="PK576_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE61980.1"
                     /translation="MMKFVAVFALMASVVIGADFQHTLSGVKPWTSEEFLGGPQEFHF
                     AVLPDRTGGERKGVFGKAMDTLNLLRPAFVMSVGDLIECGGNPDAREQWAELETHISK
                     LDMPFFYVAGNHDIWTGLKGMTPKRQKSIDLWHELHGTNTYYSFTYKGCLFLCFNTME
                     KHDYYPPREPLPENQIAWALEEIEKHKDARWRFLFMHKPIDWTSDRWLEFERKINKYD
                     YTVFCGDWHNFCTAIRHGKKYYMVGTAGGCFDRNVVQDDLRYGNMDGITWVTMTKDKG
                     PVVSHIALSGVHGDTIQTCATTLGWIETPLDYPSHLAEDPAKYQDEKNTALIPTEVMH
                     GDGYDWHFKHAIILRQGCVYGGGLEKFKAGKKRVVLLGDETASAKAAEFGEDWQVFDM
                     GFKGDKLENVMWRVIQGTLRGYDPDVVVVSAGGHNKGVNTSKEIEAGYSKLLGLVRER
                     VPKAECRIHFP"
     gene            1944..2918
                     /locus_tag="PK576_10110"
     CDS             1944..2918
                     /locus_tag="PK576_10110"
                     /inference="COORDINATES: protein motif:HMM:NF027552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOE61981.1"
                     /translation="MKAKIKLIVVATLAAFNLFSNADAIKVATETKPLKVLMIGNSFS
                     DCVLRQAPQIAQNLDCPLNITSLYIGGCPLSRHAANLDTTNAPYMVSWSYCGNSKREA
                     VPFAKVLKTRKNKDGKQRPNRFGNLRQMIAADKWDIVTIQQASHESWRPESFQPHADK
                     LIAAVRELAPQAQFRIQQTWSYCTADKRIHNPETNGPGTWGFDQTGMYERLTANYTKL
                     AKDTGFAIIPMGLAVQKFRKAKGIKSHEGDVVGNIRMENGKATGDTIHLNGAGHYLQG
                     LVWVGTLFDVDVTKCTYAPKNMDPELAKVLRACAAAAVAETRGVQTKK"
     gene            complement(2924..>3921)
                     /locus_tag="PK576_10115"
     CDS             complement(2924..>3921)
                     /locus_tag="PK576_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOE61982.1"
                     /translation="LLWKLSNGTAHVTDYTNASRTMLFNIRTLQWDAELLDLFDIPSS
                     MLPTPVPSSGEAARCVLPEFEGREVPVLGVSGDQQAALFGQGCFAPGQIKNTYGTGCF
                     LLMHTGGEAVSSANGLVTTLTAQTLAGAPSYALEGSVFVGGAVVQWLRDELCILTQAS
                     DVGPIAASIPDTGGVFLVPAFAGLGAPHWDMYARGALVGMTCGTGRAQIIRAAVESIA
                     YQCRDVVDAMRLESGQPIASLRADGGASACDFLMQFQADLLECRVERPTCIETTALGA
                     ASLAALALGWQTPETLAGRTAASVFTPSMPETQRNALLAGWRKAVSRAARWETES"
BASE COUNT          844 a         1162 c         1111 g          804 t
ORIGIN      
        1 caaacagacg ccacgccaac ctccattttg cgcaagcaca acatattgga ctgttcccgg
       61 ccgaaaatca atcccaactc catctggcga ggacgcgcgg gtgcgcgcgt gcgaggcgcg
      121 ccccccatta tcttgactgc ggtcagacac gccgacaaca aggacgattc cgatcccgat
      181 aatgattcgg atgaagctaa gtcccaacgc catccctata cccgttcgcg ttttcgtata
      241 cgtgtacgaa tcaaacgttc agcgttcaag ggaaatgaat ccggcactcg gccttcggca
      301 cgcgttcgcg gacgagtccg agcagtttcg aataccctgc ctcgatctct ttcgatgtgt
      361 tgacgccttt gttgtgaccg cctgcggaaa caaccaccac atcggggtcg tatccccgga
      421 gcgtgccttg gatgacgcgc cacatcacgt tctcaagctt gtcgcctttg aagcccatgt
      481 cgaaaacctg ccagtcttcg ccgaactcgg ccgctttcgc gcttgccgtt tcgtcgccga
      541 gcagcactac gcgtttcttg cccgccttga atttttcgag tccgccgccg tacacgcatc
      601 cctggcgcag gatgatggcg tgtttgaagt gccagtcgta gccgtcgccg tgcatcactt
      661 cggttggaat gagcgcggtg ttcttctcgt cctggtattt agcgggatct tccgcgaggt
      721 gcgacggata gtcgagcggc gtttcgatcc agcccagtgt tgtcgcgcac gtctgaatcg
      781 tgtcgccatg aactcctgaa agggcgatgt gcgagaccac cggccccttg tccttcgtca
      841 tcgtcaccca tgtgatgccg tccatgttgc cgtaacggag gtcgtcctgc acgacattac
      901 ggtcaaaaca accgccggcc gtgccgacca tgtagtattt ctttccgtgg cgtatcgccg
      961 tgcagaagtt gtgccagtcg ccacagaaca cggtgtagtc atatttgttg atcttgcgct
     1021 cgaactcgag ccaccggtcg cttgtccagt cgatgggctt gtgcatgaaa aggaagcgcc
     1081 agcgcgcgtc cttgtgcttt tcgatctcct ccagcgccca cgcgatctga ttctccggga
     1141 gcggttcgcg cggcgggtag tagtcgtgct tctccatcgt gttgaagcat agaaacagac
     1201 agcctttgta ggtgaaactg tagtaggtgt tggtgccgtg cagttcatgc cataagtcaa
     1261 tggatttctg ccgtttgggc gtcatcccct tgaggccggt ccagatatcg tggttgcccg
     1321 cgacataaaa gaacggcatg tcgagtttgc tgatgtgcgt ttcaagctca gcccactgtt
     1381 cgcgagcgtc cggattaccg ccgcactcga tgagatcacc caccgacatc acgaacgcgg
     1441 gccgaaggag gttgagggtg tccatcgctt tgccgaacac gcccttgcgc tcaccccccg
     1501 tacggtccgg gagcaccgcg aaatggaact cctgcggacc gccgaggaat tcttcgctcg
     1561 tccacggctt caccccagag agcgtatgct ggaaatcggc cccgatcacc acggacgcca
     1621 tcaatgcaaa aactgccaca aacttcatca tgtcttcatc tccctcgttt ataacgtgag
     1681 cgttttgtca cacctttcgg gcgtccgaga catccgcccg tactgttcat tgctcaaccg
     1741 gtccatgcca gtacggcggg acgtccaaag ctacgtgccg caggcgcggg accggaccgc
     1801 atccgttcag acgcgcaaaa aaacgttctt ccggcatttt attatgcaac aggctgaaaa
     1861 aagtcaaccc caaaccccgt ggcgggatgg atgagggtgt ttattgttgc ggtggatgta
     1921 tttaatcatg taccatataa atcatgaaag caaaaatcaa gttgattgtc gttgcaaccc
     1981 tggctgcgtt taaccttttt tccaatgccg atgcgataaa agtcgcaacc gagacgaagc
     2041 cgctcaaagt actgatgatc gggaactcgt tctcggactg cgtcttgcga caagcaccgc
     2101 agatcgccca aaaccttgac tgtccgctca acatcaccag cctttacatc ggcgggtgcc
     2161 cgctatctcg gcatgctgcc aaccttgaca cgaccaacgc tccgtatatg gtgtcctgga
     2221 gttattgcgg gaattcgaag agagaagccg tgcccttcgc caaggtgctg aaaacccgga
     2281 agaataagga tggtaagcaa cggccgaatc gtttcggcaa tctccggcaa atgatcgcag
     2341 cggacaagtg ggacattgtc accatccagc aggcgagtca tgagagttgg aggccggagt
     2401 ccttccagcc gcatgcggac aagctgattg ccgcagtccg cgagcttgcc ccgcaggcgc
     2461 aattccgcat ccagcagaca tggagctact gtaccgctga caagcgtatc cacaatccgg
     2521 aaacgaatgg cccaggcacg tggggctttg atcagaccgg aatgtacgag cggctcaccg
     2581 ccaactacac gaagctcgca aaggacacgg gcttcgcgat catcccgatg gggctcgcgg
     2641 tgcagaagtt ccgtaaggcg aaagggatca agagtcacga aggcgatgtc gttggtaaca
     2701 ttcgcatgga gaacggcaag gcaaccggag acaccatcca tctaaacggg gctggccatt
     2761 acctacaggg gctcgtgtgg gtcgggacgc tcttcgatgt ggatgtcacg aagtgtacgt
     2821 atgcccccaa gaacatggat ccggaactag cgaaagttct ccgggcatgc gctgctgctg
     2881 cggttgcaga aacacgcggg gttcagacaa aaaaatagtt tcgttaactc tcggtttccc
     2941 agcgcgccgc acgtgacacg gctttgcgcc atccggcgag caaagcgttg cgctgcgtct
     3001 caggcatgct tggcgtgaac acgctggcgg ccgtccggcc ggcaagcgtt tcaggcgttt
     3061 gccagccgag ggcgagggcg gccaatgatg cggcaccgag cgcggttgtt tcaatgcagg
     3121 tcggccgttc gacgcggcat tcgagcagat cggcctgaaa ctgcatcagg aaatcacagg
     3181 ctgaggcgcc accatccgca cgcagagaag cgatcggttg tcccgactcc aagcgcatcg
     3241 catcgaccac gtcccgacac tggtaggcga tcgattccac ggccgcacgg ataatttgag
     3301 cacgccccgt accgcaggtc atgccgacca gtgccccccg ggcgtacatg tcccaatgcg
     3361 gtgcgccaag ccctgcgaaa gccggcacga gaaagacccc gccggtatcg gggattgatg
     3421 ccgcgatcgg gccgacatcg gaagcttgcg tcaggataca cagttcgtca cgcaaccact
     3481 ggacaacggc accgcccaca aagacgctgc cttcaagcgc gtaagagggc gctccggcaa
     3541 gggtctgcgc ggtcagcgtt gtcaccagtc cgttcgcaga tgaaaccgct tcgccacccg
     3601 tgtgcatgag caggaaacat cccgtgccgt atgtgttttt gatctgtccg ggggcaaagc
     3661 agccttgacc aaagagcgcg gcctgctggt cacctgacac gccgagtaca ggcacttcgc
     3721 gtccctcaaa ctccggcaag acgcaccggg cggcctcgcc cgatgaggga accggggtcg
     3781 gcagcatgga tgacggtatg tcgaaaaggt caaggagttc cgcatcccat tgaagcgtgc
     3841 ggatattgaa gagcatggtt cgggaggcgt tcgtataatc ggtgacgtgc gcggtaccgt
     3901 tcgagagctt ccagagcagc c
//
LOCUS       DAOSLE010000203         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20395_length_3915_cov_6.169430, whole genome shotgun sequence.
ACCESSION   DAOSLE010000203 DAOSLE010000000
VERSION     DAOSLE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20395_length_3915_cov_6.169430"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PK576_10120"
     CDS             complement(<1..535)
                     /locus_tag="PK576_10120"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOE61983.1"
                     /translation="MKLDPTQLADWEIAEAVEPTLRPAAELAAELGLLPDEIIPYGRL
                     LAKIDATAVHARLGDPPRRAKYIDVTAITPTPLGEGKTTTTLGLVQGLGRLGRRALGT
                     VRQPSGGPTFNIKGSAAGGGLAQVVPLAPLSLGLTGDIDAITNANNLAMVALTARMQH
                     ERNYDDARLAQSRLARLD"
     gene            complement(580..2952)
                     /locus_tag="PK576_10125"
     CDS             complement(580..2952)
                     /locus_tag="PK576_10125"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HOE61984.1"
                     /translation="MLNADKTQNWKNDTLDSIDYYNDWFLRFAPLTYREQRKVKKDEV
                     ERAFVVTEYLRKLTIEVMVKHPEILSVLRMCAAPPIAQDRLAGLAYSKKSLIATMEGD
                     GGRSPSLPAKMTDAERESQLAKLLDVFNEMMDVDIFPWTVEDRLPTNDEYKMGVSIVT
                     DRLCGSAADPIIRNAQEARQLHALAVFLKHLGYQEVASSQIKKMLGKQTHFVLFLCGY
                     FDAGYLGYEAAEGIDWVWEHRIHDFLGLDLAQKDAGEDAPKETALAYRVVSASDQEAE
                     RFRRQKEVDASKSLSERNANGQYSTPFELAHVMVGQALRDRHWQSGDGAVHVFEPACG
                     SGVFLSALLAIPHAPAFLFTGVERDPDYAGICKSVFASKNVRIVEGDFFIVSETEAFQ
                     ASADVLVANPPYVRHHHLAFEDKLRLQSRVLRELGIQISGLSGLYVYFILLADRLLRS
                     GAVASWLIPGEFLYTNYGKALREYLLNRVTLLRLHTFASEDVQFDDALVSSCIVTYRK
                     APPPADAEFEVTEGRYGASGFGRKWRCAELSSFSKWVFKEQSETGKSQGVTVGDLFRV
                     TRGIATGNNGFFVLDEEHVLMVGIERDALTPLLPGPRFLRDAVIDADDQGEPQIEKRR
                     YLLSLDAQPEEVERRYPRAYQYLQEGERAGVPSGGLCKMRKLWYQQEKREPPKYLVSY
                     MGRANPVTGQSVRFFLNRSKGIATNGFICLYPRPFLVDLLSGRPDRETELLACLNAVP
                     PGHVDAAGRQYGGGLKKIEPRELSVMTVEKLPDWLCMSDQQQELFGCDKS"
     gene            complement(3000..3557)
                     /locus_tag="PK576_10130"
     CDS             complement(3000..3557)
                     /locus_tag="PK576_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61985.1"
                     /translation="MKPATLRPVLSWLAAVLLAGCDYTVPLAEKPEVPVDKALIGQWQ
                     TTLDGKDVRLSVLALAADEYLVAYPSGTPDTLYARACLCQGTEFALVQLRWFGSANAR
                     ADFSAHPYQYAAYGIEGDTLVARLINPEVVKPAQTAAALLSAIKANNTNPDLFRSELR
                     FTRVKPPADPRAPLARPPLPAGWDK"
     gene            complement(3562..>3915)
                     /locus_tag="PK576_10135"
     CDS             complement(3562..>3915)
                     /locus_tag="PK576_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61986.1"
                     /translation="AFDSSVADAVNGQATVLPRDAALCDGELIELDQTEGRVSSQLLV
                     PYPPGIPVFLPGLTITRPMIEIVRAVADAEGADAVHGLFVRGKKYYVEVIRRDEEDKI
                     QWLKERPADILFPKE"
BASE COUNT          870 a         1179 c         1053 g          813 t
ORIGIN      
        1 tgtcgagccg tgcgaggcgg ctctgcgcga ggcgcgcgtc atcgtaattg cgctcgtgct
       61 gcatgcgcgc ggtcaacgcg accatcgcca ggttgttggc attggtgatc gcgtcgatgt
      121 cgccggtgag gccgagcgag agcggcgcga gcggcacgac ctgcgcgagg ccgccgccgg
      181 cggccgagcc cttgatgttg aaggtcggac ctccgctggg ctggcgcacc gtgccgagcg
      241 cgcgccggcc gagtcggccc aggccctgca ccaggccgag cgtggtggtg gttttgccct
      301 cgcccagcgg cgtcggcgtg atcgcggtga cgtcgatgta tttggcgcgg cgcggcgggt
      361 cgcccaggcg cgcatgcacg gcggtcgcat cgattttcgc gagcagacgt ccatacggga
      421 tgatctcatc cgggagcaag cccagctcgg cggccagctc ggcggccggg cgcagggtcg
      481 gttcaacggc ctcggcgatt tcccaatcgg cgagttgtgt agggtctagt ttcattgtta
      541 tgcaccacgt tgaaggttga tatccatgcc agagagtaat cagcttttgt cacaaccgaa
      601 gagttcctgt tgttgatcgg acatgcacaa ccagtccgga agcttttcga ccgtcataac
      661 agacaattcc cgaggttcga ttttcttgag ccccccaccg tactgccttc ccgcagcgtc
      721 aacgtgcccg ggaggaaccg cattcaagca ggcgagaagc tctgtttcac gatccggccg
      781 accgctcaac aaatcaacca gaaagggccg cggataaaga caaatgaatc cgtttgttgc
      841 aatgcctttt gatcgattca aaaaaaatct gaccgactgt cctgtcacag gatttgcacg
      901 ccccatatac gaaaccaaat acttgggcgg ctcacgcttt tcctgctgat accaaagctt
      961 tcgcattttg cacaagccgc cagaagggac accggcccgt tcaccttcct gcaaatactg
     1021 atatgcgcgg ggatatcgac gttccacctc ttcaggctgg gcgtcaagcg acagcaggta
     1081 acggcgcttc tcaatttgag gttctccctg atcatcagcg tcgatcaccg catcacgtaa
     1141 aaagcgaggg cccggcaaca acggggttaa cgcatctcgc tcaatcccga ccatcaagac
     1201 atgttcctca tcaagaacaa agaatccgtt atttcccgtc gcgatgcccc gtgtcacccg
     1261 gaacaaatcg ccaaccgtca ccccttgcga tttccccgtt tcagactgct ctttgaatac
     1321 ccactttgag aatgaagaca attccgcgca ccgccatttc cggccgaaac cagaagcgcc
     1381 atatcgtcct tctgtcactt caaactcagc gtctgcagga ggcggagcct tccgataggt
     1441 gacgatgcat gacgacacca atgcatcatc aaactgcaca tcttcggacg cgaatgtatg
     1501 gagacgcaaa agtgtgacac ggtttaagag atattctcgc aacgccttgc catagttcgt
     1561 gtacaaaaac tccccgggaa tcaaccaaga ggcaaccgcc ccggatcgca gcagtcgatc
     1621 ggcaagcagg atgaaataca catacagccc cgacaaacca ctgatttgaa tccccaattc
     1681 tcgcaacacc ctgctttgca gcctgagctt gtcctcaaac gccagatgat gatggcgaac
     1741 atacggcgga ttggccacca agacatcggc agaagcctga aaggcctcgg tctccgaaac
     1801 gatgaaaaaa tccccctcga caattctcac attctttgac gcgaacaccg atttacagat
     1861 gcccgcgtaa tcaggatcac gttcaacacc tgtgaacaaa aaagcaggcg catggggaat
     1921 ggccagcaac gcagacagaa acacgccgct tccacacgcc ggctcaaaga catgcaccgc
     1981 gccatcccct gattgccaat gccgatcgcg caaagcctgc cccaccatca catgcgccaa
     2041 ttcaaagggc gttgaatatt gtccgttcgc atttctttcg gaaagcgact ttgacgcatc
     2101 gacttctttt tgccgcctga accgctccgc ctcttggtca gaagcggaaa ccacacgata
     2161 tgccaatgcg gtttcttttg gagcgtcctc accggcgtct ttctgcgcca gatctaatcc
     2221 taaaaaatca tgaattctat gttcccatac ccaatcaatt ccttcagcag cctcgtatcc
     2281 cagatacccc gcatcaaagt aaccgcataa gaacaggaca aaatgtgtct gtttacccaa
     2341 catcttcttg atctgagaag atgcaacctc ttgataccct aaatgcttca ggaacacggc
     2401 cagtgcatga agttggcgtg cttcctgtgc attacggata atcgggtcgg cagcagagcc
     2461 gcacagacga tccgtaacga tggaaacccc cattttatat tcgtcgtttg tcggcaacct
     2521 gtcctcgacc gtccaaggaa aaatgtcaac gtccatcatc tcgttgaaaa catccaacag
     2581 tttggccaac tgagattccc gttcagcgtc tgtcattttg gcaggcagac tcggggatct
     2641 tccaccgtcc ccttccatag tagcaatcag actttttttg gaatacgcca agcctgccaa
     2701 tctgtcctgc gcaatcggag gggccgcaca catccgtaaa acagaaagga tctccggatg
     2761 tttaaccatg acttctatcg tcagcttccg taaatattct gtaacaacaa acgcacgttc
     2821 gacctcgtct ttcttgacct ttctctgctc gcggtaagtg agtggggcaa aacgaagaaa
     2881 ccaatcgtta taataatcga tggaatccag ggtgtcgttt ttccaatttt gtgttttgtc
     2941 agcgttcaac atgccgccac acctcttttt cacccgaagt ctttccgaca cactgctcgt
     3001 cacttatccc acccggcggg gagcggcggg cgggcgagcg gcgcgcgggg atctgcgggc
     3061 ggtttgacgc gggtgaagcg cagctccgat cggaagaggt cgggattcgt gttgttggct
     3121 ttgatggcgc tcagcagcgc ggcggcggtc tgcgccggtt tcacgacctc ggggttgatc
     3181 agacgcgcga ccagcgtgtc gccctcgatg ccgtaggcgg catactggta gggatgggcg
     3241 ctgaagtcgg cacgggcgtt ggcggagccg aaccagcgga gctgcacgag cgcaaactcg
     3301 gtgccttggc agaggcaggc gcgcgcatag agcgtatccg gcgtgccgga gggataggcg
     3361 accagatatt catcagccgc gagggccaac accgacagcc gcacatcctt gccgtccaag
     3421 gttgtttgcc actggccgat cagagccttg tcgaccggga cctcgggctt ttccgcgaga
     3481 ggcaccgtgt agtcgcatcc tgccagcagg acagcggcca accaactgag cacggggcgc
     3541 aacgttgcag gtttcatagc cttactcctt ggggaacagt atatcagcgg gacgttcttt
     3601 caaccactgg attttatcct cctcgtcgcg gcggatcacc tccacatagt acttcttgcc
     3661 gcgcacgaag agcccgtgca ccgcgtccgc gccctcggca tcagccacgg cccggacgat
     3721 ctcgatcatc ggccgcgtga tggtgaggcc gggcaggaag accgggatgc ccggcggata
     3781 aggcacgagc aactggctgc tgacgcggcc ttcggtctgg tcaagttcga tcagctcgcc
     3841 gtcgcacagg gcggcgtcac gcggcagcac agtggcctgg ccgttcacgg cgtcggccac
     3901 gctgctgtcg aaggc
//
LOCUS       DAOSLE010000204         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20475_length_3903_cov_7.875000, whole genome shotgun sequence.
ACCESSION   DAOSLE010000204 DAOSLE010000000
VERSION     DAOSLE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20475_length_3903_cov_7.875000"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1723)
                     /locus_tag="PK576_10140"
     CDS             complement(257..1723)
                     /locus_tag="PK576_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE61987.1"
                     /translation="MGVGGERGAVLPGGYREMWRIATPLILSMASYTIMQFCDRVFLA
                     RYSSVTIQAALPAGALAHTLICFFQALAGYAGTFTAHYYGANDPVQCVRATIQGIWLA
                     LGSWPLILLQIPLGCGIMTLFGHMPEVLEAEKTYFLILMAGGITVPLNAAVGGYFMGI
                     GKTSVNMKSNLIGCSLNVVLNYVMIFGKWGCPELGIAGAAYATLISSAVTCLLQGLPF
                     YQACRICMEERRNASRPDSSAGTLFSDFWFPDYRMLSRIVRYGTPSGLQLLMDVGSFA
                     VFIMLTGRLGEVALAASNIAFSINNLAFAPLLGFGLAASMLVGQHQGAGRSDAAERSG
                     YTGLKMGLAYMCVIGSTFVLFPHLYFELFRPPDAAFSTAELLGTGRVMLWLMTIWGVL
                     DTVSIVLSGALKGAGDTHFVMVYMIIGSWLFLVPGSLVLLRLGFGILGLWVWMAVYIS
                     VLASGFWWRWRTGRWKTIRVIDRREGLDYIPLVTDEIH"
     gene            complement(1870..2895)
                     /locus_tag="PK576_10145"
     CDS             complement(1870..2895)
                     /locus_tag="PK576_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOE61988.1"
                     /translation="MTTVLALMHTQEDGSLPKAAREALTAASQLAAQSGAALSVGVIG
                     KGASAAADTVAGCGAASFWVIGDEAVYPARYATDAAAVTALVKAAGAQIVVAPGSSRF
                     MRCLPGASYRLGAVIDTHIVAQQAAPDGSVRVQRWFYRQRILGSFSRAARPWLLVVDS
                     GVNAPWEGAAGSASAQTVEVAFAEDGQRTTVTGARAPAADAQTIRPDAPLLFVAGAGW
                     TKKQKDGQPHIAEASGLIQTFLGKTQASLGSSKSLVDIQSEGQETLHFMSHMNQIGQT
                     GSTPRHPKGLATCCHGEEPHAVGWRFINERRAVNLDPNCGWAQGKADVVYVADAFDVM
                     ARVNELL"
     gene            complement(2916..3725)
                     /locus_tag="PK576_10150"
     CDS             complement(2916..3725)
                     /locus_tag="PK576_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HOE61989.1"
                     /translation="MTDGFQLVVCGSLVPDPLQTLEPVSTPQGPGLKNEMMLPSVLDP
                     WAAHALYEAADLAKRFPGSKVWLVSLGPKAKLQQLMMTVAQKVPFELIALDGSASGFT
                     DALGVAEALAGAIEAIPGLDKSRLLVFGGCASASRDAGVTLQVVGERLGIRDQFVGVD
                     MLTVRPEGGFEVLERIEGGQYQVSVCAGAPAVLAWATGNLPEPPNNPQIGMANMRGVM
                     PALQKAKPAAAGVGGMAYGDVELPSQKRQTRIVKDATADEIAREIVEWIKA"
BASE COUNT          792 a         1387 c         1063 g          661 t
ORIGIN      
        1 tcaaggtttc agggtttcag ggtttcatcc caatcggggt cgggatcgct atcgggatcg
       61 tcgacccgcg acccgcgacg atcgacaggg acgcccgcgt ttccgacacc gcaggtcgct
      121 cgtcgcggtt cgcgggtcga catataaggt ctgacgggag aagcgggcgt cccgcatacg
      181 caccgcaaaa cccaatgttt gcggggcttt gctagaattc tacccgttca actgctcctt
      241 ttagactaaa acaagctcag tgaatttcgt cggtgaccag cggaatataa tcgaggcctt
      301 cccggcgatc gatcacacgg atcgttttcc aacggcccgt tcgccagcgc caccagaacc
      361 cggaggccag aaccgaaata tagacggcca tccataccca caaccccagg ataccgaaac
      421 cgaggcgtag caagaccagc gaaccgggaa ccaaaaacag ccagcttccg atgatcatgt
      481 agaccatgac gaaatgcgtg tctcccgcgc ctttcaacgc gccgctcaag acgatcgaca
      541 cggtgtcgag cactccccag atggtcatga gccacagcat gacgcgtccg gttcccaaca
      601 gttccgcagt ggagaacgcc gcatcgggag gacggaaaag ttcaaaatac aggtgcggga
      661 agagtacaaa agtggaaccg atcacacaca tataagcgag tcccatcttg agcccggtat
      721 atcccgatcg ttcagccgcg tccgaccgtc cagccccctg atgctgcccc accagcatgg
      781 aggccgccaa cccgaacccc agcagcgggg cgaaagccag gttgttgatg ctgaacgcga
      841 tgttgctggc cgccaacgcc acctcgccca gccgtcccgt cagcatgatg aagacggcga
      901 acgacccgac atccatcagt aactgcagac ctgatggcgt gccgtaccgc acgatgcgac
      961 ttaacatccg gtaatccgga aaccagaagt cggagaacag cgttcccgcg gaagaatccg
     1021 gacgcgaagc gttccgcctc tcctccatgc aaatccggca ggcttgataa aacgggagcc
     1081 cttgcaagag gcaggtgacc gcactggaga tcagtgtcgc gtatgccgcg cccgcgatgc
     1141 cgagttccgg gcacccccat tttccgaaga tcatgacgta attgagcacg acgttgagcg
     1201 aacagccgat gagattcgac ttcatgttga cgctcgtctt cccgatgccc atgaagtacc
     1261 cgcccacagc ggcgttcaac ggaacggtta tcccgcctgc catcaaaatc aggaagtacg
     1321 ttttctcagc ctcgagcact tcaggcatat ggccgaagag ggtcatgatg ccgcacccga
     1381 gcggaatctg cagcaggatc aggggccagg agccgagcgc cagccagatg ccttggatcg
     1441 tggcgcgcac gcattgcacc ggatcgttcg ccccatagta atgggcggtg aaagtgccgg
     1501 cgtatcccgc gagcgcttga aagaaacaga tcagcgtgtg cgcgagagcg cccgccggca
     1561 aagccgcttg aatcgtcacg ctgctgtaac gcgcaagaaa cacgcggtcg cagaactgca
     1621 tgatggtgta ggacgccata ctgaggatga gcggagtggc gatccgccac atctcccggt
     1681 atccgcccgg caacaccgcg ccacgctctc cccccacgcc cacacgcccc cttcccgggc
     1741 cccgtccttc cgaccctggc tgtatcaccc tgacaggatc acaccggcaa cccggacagc
     1801 gcggggcgat ccgcctgcgc gtcgccccac acctccggac tgccggtccg ggcctgcggc
     1861 ccgcacggcc tacaacagct cgttgacccg cgccatcaca tcaaacgcgt ccgccacata
     1921 gaccacatcc gccttgccct gcgcccatcc gcaattcgga tcgagattga ctgcgcggcg
     1981 ttcgttgatg aaacgccagc ccaccgcatg cggctcttcg ccgtggcagc aggtcgccaa
     2041 ccctttcgga tgacgcggtg tggaacccgt ctggccaatc tggttcatat gcgacatgaa
     2101 atgcagcgtc tcctgaccct cgctctggat gtccaccagc gacttgctgc tgccgagcga
     2161 ggcctgtgtc ttccccagaa acgtctggat caacccactg gcctccgcga tatgcggctg
     2221 gccgtccttc tgcttcttcg tccacccggc tcccgccaca aacagcagcg gggcgtccgg
     2281 acggatcgtc tgcgcgtccg ccgcaggggc gcgggccccc gtgaccgtcg tgcgctgccc
     2341 atcctccgca aacgcgacct ccaccgtctg tgccgacgcg ctcccggcgg caccctccca
     2401 cggcgcattg accccgctat ccaccaccag cagccacggc cgcgccgcac ggctgaaaga
     2461 acccaaaatc cgctgacggt aaaaccagcg ctgcacgcgc accgaaccgt ccggcgccgc
     2521 ctgctgcgca acgatgtggg tgtcgatcac ggcacccaga cggtacgacg cgcccggcag
     2581 gcaccgcatg aaccgcgaac tccccggcgc caccacgatc tgcgcgcccg ccgctttgac
     2641 caaagccgtg accgccgcgg catccgtcgc gtagcgcgcc ggatacaccg cctcatcgcc
     2701 gatcacccaa aacgacgcgg caccgcagcc cgccaccgta tcagcagccg ccgacgcccc
     2761 ttttccgatc acaccgaccg acagcgccgc gccggattgc gcggcgagtt ggctcgcggc
     2821 cgtcagcgcc tcccgcgccg ctttgggcag actgccgtcc tcttgcgtgt gcatcaacgc
     2881 caaaaccgtt gtcatatcca atcctccctg ttccgctacg ctttgatcca ttccacgatt
     2941 tcgcgggcga tctcatccgc cgtcgcatcc ttcacaatcc tcgtctggcg tttttgcgac
     3001 gggagttcga catccccgta ggccatgccg cccacgcccg ccgccgcagg tttggccttt
     3061 tggagcgcag gcatcacccc gcgcatgttg gccatgccga tctgcggatt gttcggcggt
     3121 tccggcaggt tgccggtcgc ccaagccaaa accgctggcg cccccgcgca aaccgaaacc
     3181 tgatattgcc ctccctcaat ccgttccaac acctcgaaac cgccttcggg ccgcacggtc
     3241 agcatatcga cgccgacaaa ctgatcgcgg atgcccaacc gctcgcccac cacctgcaac
     3301 gtcacacccg cgtcacgcga ggccgacgcg catccgccga aaaccagcag gcgggatttg
     3361 tccagaccgg gaatcgcctc aatggccccg gccaaagcct ccgccacgcc caacgcatcc
     3421 gtgaagccgc tggcgctgcc gtccagcgcg atcaactcaa acggcacctt ctgggccacg
     3481 gtcatcatca attgctgcaa cttggccttg gggccgaggc tcaccagcca gaccttgctg
     3541 ccgggaaacc gcttcgccaa gtccgctgcc tcgtacagcg catgcgccgc ccacgggtcc
     3601 agcaccgaag gcagcatcat ttcgtttttc aatccgggac cctgcggggt ggacaccggt
     3661 tcgagcgtct ggagcggatc aggcaccaga ctaccgcaca cgacaagttg aaatccgtct
     3721 gtcatcgttc tctcctttgc cacacactat agcagatggc cctcaccggg tggaatgccc
     3781 tttttccgcc cgcgccgcag gcggtaaatt cggttcttgc caccggtccg catgaaacgg
     3841 cataatgcgc acgcttttcg gggcggttat ctcagttggt tagagagtct ggtttacacc
     3901 cag
//
LOCUS       DAOSLE010000205         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20488_length_3902_cov_8.540421, whole genome shotgun sequence.
ACCESSION   DAOSLE010000205 DAOSLE010000000
VERSION     DAOSLE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20488_length_3902_cov_8.540421"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1227
                     /locus_tag="PK576_10155"
     CDS             139..1227
                     /locus_tag="PK576_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOE61990.1"
                     /translation="MTVTLPEQCLHDPVSGIWRTCLPTVARLSSSDTLMLDADSVIRC
                     DGAGEALILELHRRAASQGFTLQCRDARGVVARTLERFRVERFTSSLATPHPVRHTRL
                     CEQLGRTCVHLATGFRDQTAFLGEATVSALSLPRQAGRFRLRDALLHFERAGIDALPI
                     VLLIGFLLGLILAFMSATSFRQFGVEVYVADLIAIGLFRELGPLITAILLAGRSGSAF
                     AAEIGTMKVNEEIDALVTLGLRPVTFLALPRILAATLVMPILSTFSVLAGLLGGVLVM
                     AALGVPPLVFWQHAIRATSLNNVLFGLVKAAVFGLLVGLVGCAHGLQTRRAADGVGRA
                     ATAAVVGSLVLITVADGLFAVLAYLLGV"
     gene            1229..2014
                     /locus_tag="PK576_10160"
     CDS             1229..2014
                     /locus_tag="PK576_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE61991.1"
                     /translation="MTGSPVIEVEHVCAGYDTALLDDVSFQVNRGEVFVILGGSGCGK
                     STLLKHLIGLIPPLGGTIRLMGDRLFGSPEAERERAMRRFGVTYQSGALFGSMTLLEN
                     VCLPLESFTALPREARDLLAEMKLDLVGLKAFADFFPADISGGMKKRAAIARAMALDP
                     EILFLDEPSAGLDPVTSAELDRLILQLRDTQGVTVVMVTHELASIHAVADRCVMLDKT
                     ARGIIAEGPPAELRDRPPNATVRAFFQREGNRTGASGSHEVSK"
     gene            2011..3123
                     /locus_tag="PK576_10165"
     CDS             2011..3123
                     /locus_tag="PK576_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE61992.1"
                     /translation="MNENKANYAKIGFFVLAGMVLMIIVIAIAGSRGLTKRAILAETF
                     FSESVTGLDIGSPVKYRGVPVGEVKHIGFVYSAYREQAHEELTPARARQILVVMALDP
                     ERFGLLGSERADAVLNQLVAQGLRVKIASSGMTGLAFLEMDYFKGQPSEPAPAELQLE
                     THHPYIPSVASTMTALKKAVDDVFVKLSAVDLQALGDEFLATLYLVQHALAHTDVATL
                     AEETTALMTELRESNRSLKGLLDSPELGRLPSTLAATAESARRTIERIESRIDPMTAS
                     VTNVTGRAVCLMEQANGLLEQHGAQIGVTVEALARTAQTLDRTAATQQGDVAALLEDL
                     RRAADGLERIISNLTSAPSALLFGRPPAPLPETRKP"
     gene            3149..3784
                     /locus_tag="PK576_10170"
     CDS             3149..3784
                     /locus_tag="PK576_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOE61993.1"
                     /translation="MNRITSLCISLIVLAGSSGCLGGARAHQIADRQKRSFLLEASAA
                     QPLAPAPRFGAALLRSLRVQPPFDARTFLIRRGQNEFAADFYHGWIAPPAVLIRSQTA
                     RYLRETGLFGTVFDQPGGTRVPLGLECIVSDLYLDHSGEQPAAVVAMRVLVLDERTTD
                     FPVLFTCEKQARVPLASSDAGTAAEAFGLALTQTLEAVAQSLATAPLSEIR"
BASE COUNT          667 a         1281 c         1188 g          766 t
ORIGIN      
        1 cgcggtttcc gcttttgctc tatccaaccg tccgcttttg aaattcccac gacacaaaaa
       61 acgattgcgg gctttgcccg catctccgac tttgatacgg tataaggatt caactgtgac
      121 gaagtgaaac gctgacccat gaccgtgact ctcccagaac agtgtctgca cgacccggtg
      181 tccggcatct ggcggacttg tcttccgact gtcgcgcgcc tctcttcgtc ggatacgttg
      241 atgctggatg cggacagcgt gatacggtgt gacggggctg gcgaagccct gattctggaa
      301 ctccaccgcc gcgccgcgag tcaggggttc acgctccaat gccgggatgc gcggggggtt
      361 gtggcgcgca cgctcgaacg gttccgtgtc gagcgtttca cctcctccct tgcgacgccc
      421 catccggtgc gccacacgcg gctgtgcgaa cagctcgggc gcacctgcgt gcacctcgcc
      481 accggattcc gtgaccagac tgcgtttctg ggcgaggcca cagtctcggc cctttccctg
      541 ccgcggcaag ccggacggtt ccggttgcgg gacgccctgc tgcatttcga acgcgcggga
      601 atcgatgcgc tgcccatcgt gttgctgatc ggttttctgt tggggctgat cctggctttc
      661 atgtccgcca cctccttccg gcaattcggg gtcgaggtct atgtcgccga tctgatcgcc
      721 atcggcctgt tccgggagct gggcccgctc atcaccgcaa ttctcctcgc gggccgctcc
      781 ggttccgcgt tcgccgccga aatcggcacc atgaaggtga acgaggagat cgacgcgctg
      841 gtgaccttgg gcctgcggcc ggtcaccttt ctggcgctgc cgcgcatcct tgccgccacg
      901 ctcgtgatgc cgatcctcag caccttttcc gttctcgcgg gacttttggg tggtgttctg
      961 gtgatggccg cgctcggcgt cccgccgctc gttttttggc agcatgcgat ccgggcgacc
     1021 tccctgaaca acgtcctgtt cggtctcgtc aaagcggcgg ttttcgggtt gctggtcggg
     1081 ctcgtgggct gtgcccacgg attgcagacc cgccgcgccg cggacggggt cggccgcgcg
     1141 gccaccgcgg cagtggtagg cagcctcgtg ttgatcaccg tggcggacgg cctgttcgcc
     1201 gtgctcgcgt atctgctcgg agtgtaacat gaccggatcc cctgtcatcg aagtcgaaca
     1261 tgtgtgtgcc ggatacgaca ccgccctgct cgatgacgtt tcgtttcagg ttaaccgggg
     1321 tgaggtgttc gtcatcctcg gcggatcggg gtgcggcaaa agcaccctgc tgaagcatct
     1381 catcggcctg atcccgccgc tgggcggcac catccggctg atgggcgacc ggctgttcgg
     1441 cagcccggaa gcggaacgtg aacgggccat gcgccgattc ggcgtgacct atcagagcgg
     1501 ggccctgttc ggatcgatga cgctcttgga aaatgtctgc ctgccgctcg aatccttcac
     1561 cgcgttgccg cgcgaagcgc gggatctcct ggcggaaatg aaactcgatc tggtcggact
     1621 caaggcgttt gcggatttct ttccggcgga tatcagcggc ggcatgaaaa aacgcgccgc
     1681 gatcgcgcgg gccatggcgc tggacccgga aatcctgttt ctggacgagc cgtccgcagg
     1741 tctggacccc gtcacctccg ccgaactcga caggctcatt ctccagttgc gcgacacgca
     1801 gggcgtaacc gtcgtgatgg ttacgcatga actcgccagc atccatgcgg tggcggaccg
     1861 ttgcgtgatg ctggacaaga ccgcacgcgg cataatcgcc gaaggtccgc ccgcagaact
     1921 ccgcgaccgg ccgccgaacg ccacagtgcg cgcgtttttc caacgggaag gaaaccggac
     1981 cggggcatcc ggttcacacg aggtgtccaa atgaacgaaa acaaggccaa ttacgcgaag
     2041 atcgggtttt tcgtcctcgc tggcatggtg ctgatgatca tcgtgattgc gatcgccgga
     2101 tcgcgcggtc tgacgaagcg ggccattctg gcggagactt ttttctccga atccgtgacc
     2161 ggtttggaca tcgggtcccc ggtgaaatac cgcggcgtgc cggtcggcga agtcaagcac
     2221 atcgggttcg tgtacagcgc gtaccgggag caggcgcacg aagaactgac ccccgcccgg
     2281 gccagacaga ttctggtcgt tatggctctg gatccggaac gcttcggcct gctgggctcc
     2341 gagcgggccg acgcggttct gaaccaattg gtcgcgcagg gcctgcgggt caaaatcgcc
     2401 agttccggca tgacgggcct cgcctttctg gagatggatt acttcaaggg acagccgagc
     2461 gagccggcac cggccgaact tcaactggaa acccaccacc cctacatccc ctcggtcgcc
     2521 agcaccatga ccgctctgaa aaaagcggtg gatgacgtgt tcgtcaaatt gagcgctgtt
     2581 gaccttcagg ccttgggcga cgagtttctc gcaacgctct atctggtaca gcatgcgctc
     2641 gcccacacgg atgtggccac gctggcagag gaaaccaccg cactgatgac ggaactgcgg
     2701 gaatccaacc gctcgctgaa aggcctgctg gattcgccgg aacttggccg ccttccgtcc
     2761 acccttgccg caaccgccga aagcgcgcga cgcaccatcg agcggatcga atcgcgaatc
     2821 gaccccatga ccgcttcggt caccaacgtg accggccgcg ccgtctgcct gatggaacag
     2881 gcgaacggcc tcctcgaaca gcacggcgca cagatcggcg tgacagtgga ggccttggcc
     2941 cggaccgccc aaaccttaga ccggaccgcc gcgacacaac aaggggatgt cgccgcgtta
     3001 cttgaagatc ttcggcgcgc ggccgatgga ctggagcgga tcatcagtaa cctgacctcc
     3061 gccccgtcgg ccctgctctt cggtcgcccg cccgcaccgc ttccggaaac ccggaaacct
     3121 tgaaacccgc cacacaggaa aaaaccccat gaaccggata acctcgctct gcatctcgct
     3181 catcgtactg gccggatcat ccggctgcct cggcggcgcg cgcgcgcacc agatcgccga
     3241 ccgccagaaa cgctcgtttc tgctggaggc ctccgccgcc caacctctgg cacccgcccc
     3301 ccgctttggc gcggccttgc tacgctccct gcgggttcag ccgccattcg acgcgcgcac
     3361 cttcctgatc cggcgcggcc aaaacgaatt cgccgccgac ttttatcacg gctggattgc
     3421 cccgcccgca gtcctgatcc gcagccagac cgcgcggtat ctgcgtgaaa ccggtctgtt
     3481 cgggaccgta ttcgaccagc ccggcggcac acgcgttccg ctggggctgg agtgtatcgt
     3541 aagcgatctg tacctcgacc actccgggga gcagcccgcc gcagtcgttg caatgcgggt
     3601 gctggttctt gatgagcgca ccacggattt tcccgtgctg ttcacctgcg agaagcaggc
     3661 gcgcgttccg ctggcctctt ccgatgcggg cacggccgcg gaagcgttcg gcctcgccct
     3721 gacgcaaaca ctggaggccg tggcacaatc attggccacc gcaccactca gcgagatccg
     3781 ctaaaccgaa aaagagcagt tgaaccttca accctctttg gtttcaaggt ttcagggttt
     3841 ctgggtttca aggtttcagg gtttcagggt ttcatcccaa tcggggtcgg gatcgctatc
     3901 gg
//
LOCUS       DAOSLE010000206         3865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20740_length_3865_cov_6.223885, whole genome shotgun sequence.
ACCESSION   DAOSLE010000206 DAOSLE010000000
VERSION     DAOSLE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20740_length_3865_cov_6.223885"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK576_10175"
     CDS             complement(<1..308)
                     /locus_tag="PK576_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61994.1"
                     /translation="MNLKPIFLSLIVLTWCGCSTYDAQQTRSEHTEDFRRDLDAQTAA
                     LLEQPLSLQDCLTIAMTNNYEARQADLRHELARLGKDIAFAEFLPKMTLAASATRNRF
                     P"
     gene            802..3630
                     /gene="uvrA"
                     /locus_tag="PK576_10180"
     CDS             802..3630
                     /gene="uvrA"
                     /locus_tag="PK576_10180"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE61995.1"
                     /translation="MTDILISGARQHNLKNVDVRIPRDSLTVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLDRMQKPDVDHIEGLSPAIAIEQRTSGGNPRSVVATVT
                     EIHDYLRLLYGSIGQAHCPECGKPVGRQSAEQIVDRLSGEPAGSKLILLAPLVRGRKG
                     RHEEVLEAARRQGFVRIRVDGNLFALDDVPRLEKNKAHTLEVVVDRLVAGDGVHARLT
                     DSVELGLKQGNGLIQVQVTRPDGKTREEVFSEKNACPDCGISFEELKPRSFSFNNPYG
                     ACPVCAGLGAQLVFDEERVVPNKTLSLAEGAIQPWRHGGFRMVVYYKHLTKAVAAAYG
                     VDLNTPYQELPERFRQILLHGSGEEPVTLRFWMRGAWRKTEKPFEGVLPSLLRRYNES
                     EFDEVREKLRQYMSRQTCPACQGARLRPEARVCTVSGRSLVEVMAMTIRQGVAFFDKL
                     KLDAAQVAAVSEVLKEIRRRLAFLSDVGLDYLSLDRESSTLSGGEMQRIRLATQIGSG
                     LVGVLYVLDEPTIGLHPRDNERLIGMLRRLQRRGNTVVVVEHDEEMIRNADHIVDLGP
                     RAGRQGGEVVYQGGLKGLLASRHSLTADYLTGRMRIAIPEARVQPGPARLTIKGAAEN
                     NLRNIDVEIPLGCFVCVTGVSGSGKSTLVGEILKNYLFRHFYGSKERVGTCKKITGIE
                     GIDSVIEIDQSPIGRTPRSNPVTYTGAFTPIRSLFAATPAAKVRGYGPGRFSFNVKGG
                     RCEVCKGDGVRCLEMHFLPDVYVTCEQCGGQRYNKETLEVRYGGRNISEVLNLTVDEA
                     LEFFAPVPAVAKKLRTLSEVGLGYIHLGQASTTLSGGEAQRIKLAAELSKRSTGRTLY
                     LLDEPTTGLHFADIHKLLDLLLRLRESGNTVVVIEHNPDVIKCADHLIDLGPGGGDEG
                     GWLVATGTPEEVAACPDSVTGRFLKRHL"
BASE COUNT          706 a         1024 c         1360 g          775 t
ORIGIN      
        1 gggaagcggt tgcgcgtggc cgaggcggcc aacgtcattt tcggcaggaa ctccgcgaac
       61 gcgatatctt tgccgaggcg ggcgagttcg tgccgcaggt cggcctggcg cgcctcgtag
      121 ttgttggtca tcgcgatcgt cagacagtcc tgcaaagaga gcggctgttc cagcagggcg
      181 gcggtctgcg cgtcgaggtc acgccggaaa tcttcggtat gctcgctccg cgtctgctgt
      241 gcgtcatagg tgctgcatcc acaccatgtg aggacgatca gagaaagaaa aataggctta
      301 agattcattg tcaatgtttg tgggttgccg ggcctatccc gggttgaacg gggcgggata
      361 atacaggttc ggattatccg aatcaagacc aaatttgtgt tttttaacgc agagacgggg
      421 agacgcagag ggttgagaga agttcattat tcattagttg attagttcat tagtgcatta
      481 gttttggcat gatcgcaggt cgcgggtcga atgtcgcggg tcaacctctc ttattgcggc
      541 cacccagtgg tcgcaaccgt gagagtaaaa cagggtgacc atctcagggc gacgccgaat
      601 aggggcacgg gttttccaaa taccgggaag gcgctcatac catgaccgcg aaacgctggc
      661 ttggatcgcg tgcggaaaag agcggttgac gcctttgggg aggctccgta cacttccttc
      721 cctatcaggg gatcaggcgg cggacgcgga cggtctccgg aggcctggcc gggggcggac
      781 ttgtccggcg cggtgatatt tatgacagac atcctcatca gtggcgcgcg gcagcataat
      841 ctcaagaatg tcgatgtgcg catcccgcgt gattcgctta cggtgatcac cgggctcagc
      901 ggttccggca aatcgtcgtt ggcgtttgac acgctctatg ccgaggggca gcgccggtat
      961 gtcgagagtc tctcggccta tgcgcggcag tttctggacc ggatgcagaa gcccgacgtg
     1021 gaccatatcg aagggctgtc gcccgcgatc gcaatcgagc agcggacatc cggcgggaat
     1081 ccccgctccg tcgtggccac ggtgacggag atccacgatt atctgcgcct gctgtacggc
     1141 agcatcggac aggcgcattg tcccgagtgc ggcaaaccgg tagggcggca gagcgcggag
     1201 cagatcgtgg accgcctgtc gggcgagcca gcggggagca agctgattct gctggcacct
     1261 ttggtgcggg gacgcaaggg tcggcatgag gaggtgctcg aggcggcccg gcgtcagggt
     1321 tttgtccgga tccgtgtgga tgggaacctg tttgcgttgg acgacgtgcc gcggctggag
     1381 aagaacaaag cgcacaccct tgaggtggtg gtggaccggc tggtggcggg ggacggggtg
     1441 catgcgcggc tcaccgactc ggtcgagttg gggctgaaac agggtaacgg cctcattcag
     1501 gtgcaggtga cccggccgga cggtaagacg cgcgaggagg tgttttccga aaagaatgcg
     1561 tgtccggatt gcgggatcag ttttgaagag ctaaagccgc ggtcgttctc cttcaacaat
     1621 ccgtatggcg cctgtccggt ttgtgcggga ttgggcgccc aattggtttt tgacgaggag
     1681 cgggtggtgc cgaacaaaac cctgtcgctg gccgaaggcg cgattcagcc ctggcgtcac
     1741 ggcgggttcc gcatggtggt ttactacaaa cacctcacca aggcggtggc tgcggcgtac
     1801 ggggtggatt tgaacacgcc gtatcaggag ttgcccgagc gtttccggca gattctcctg
     1861 cacggtagcg gggaggagcc ggtgacgctg cggttctgga tgcgcggtgc ctggcgcaaa
     1921 accgaaaagc cgttcgaagg cgtgctgccg agtctgttgc ggcgctacaa cgaatcggag
     1981 tttgacgagg tgcgggaaaa actccggcaa tacatgagcc gtcagacctg tccggcctgt
     2041 caaggggcgc gtctgcgtcc cgaggcccgc gtctgcaccg tgtcaggccg ttcgctggtg
     2101 gaggtgatgg ccatgacgat ccggcagggg gtggcgtttt tcgacaaact gaagttggat
     2161 gcggcgcagg tggcggcggt ctccgaggtg ttgaaagaga ttcgccgccg gctcgcgttc
     2221 ctttcggatg tggggctcga ttatctctcg ctcgaccggg agagttcgac cctttccggc
     2281 ggcgagatgc agcgcattcg gctggccacg cagatcgggt ccgggctggt cggtgtgttg
     2341 tatgtgctgg acgagccgac gatcggtctt catccgcgtg acaacgagcg gctcatcggc
     2401 atgctgcgcc ggctgcagcg gcgcggcaac accgtggtgg tggtcgagca tgacgaggag
     2461 atgatccgaa atgcggacca cattgtggat ttggggcccc gggccggacg tcagggtggc
     2521 gaggtggtgt atcagggagg gttaaaggga ctgctggcca gcaggcattc cctgacggcc
     2581 gattacctga ccggacgcat gcggatcgcg atccctgagg cgcgcgtcca accgggtccg
     2641 gcccggctca ccatcaaggg ggctgcggaa aacaatctcc ggaacatcga tgtggagatc
     2701 ccgctcggtt gtttcgtctg tgtgacgggg gtctccgggt ccggcaaatc cacactggtg
     2761 ggcgagatac tcaagaacta cctgttccgc catttctacg gttccaaaga gcgcgtgggg
     2821 acctgcaaga aaatcaccgg gatcgaaggg atcgacagcg tcatcgagat tgatcagagc
     2881 ccgatcgggc gcacgccccg cagcaaccca gtcacctaca ccggggcctt cacaccgatc
     2941 cgcagcctgt tcgccgccac gcccgcggcc aaggtgcgcg ggtacggtcc ggggcgcttc
     3001 agcttcaacg tcaagggcgg tcggtgcgaa gtgtgcaagg gggacggcgt gcgctgtctg
     3061 gaaatgcact ttctgccgga tgtgtacgtg acctgcgagc aatgcggcgg gcagcgttac
     3121 aataaagaga cactggaggt ccggtacggt ggcaggaaca tctccgaggt gttgaatctg
     3181 acggtggacg aggcgctcga gttctttgcc ccggtgccgg cggtcgccaa gaaactgcgg
     3241 actctatccg aggtcgggtt gggatacatt catttgggtc aggcctcgac gaccctttcg
     3301 gggggcgagg cgcagcgcat caagctggcc gccgaactgt cgaagcgctc gaccggccgg
     3361 acgctgtatc tgttggacga gccgacgacc ggcctgcact tcgcggatat tcacaaactg
     3421 ctcgatctcc tgttgcggtt gcgtgaatcc ggcaacacgg tggtggtgat cgaacataat
     3481 ccggatgtga tcaaatgtgc ggatcacctc attgatctcg ggccgggcgg gggggacgag
     3541 ggcgggtggc tggttgcgac cggcacgccg gaggaggtcg ccgcctgccc ggactccgtg
     3601 acgggacgct ttctgaagcg gcatctgtga ggcgcgctga cgctttttgg ggggagggaa
     3661 cgtgggatgt gtccgtcatg cctgtcgcga agcatgggcg ggggacggtt ccctgaaccg
     3721 tccgcggacg cttcggggag gcgtccctac cgtgaaccta aaaagagcag ttgaaccttc
     3781 aactctcttg ggtttctggg tttcaaggtt tctgggtttc agggtttcat cggggtcggg
     3841 atcgggatcg ctatcgggtt tctgg
//
LOCUS       DAOSLE010000207         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20846_length_3849_cov_9.058777, whole genome shotgun sequence.
ACCESSION   DAOSLE010000207 DAOSLE010000000
VERSION     DAOSLE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20846_length_3849_cov_9.058777"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1639
                     /locus_tag="PK576_10185"
     CDS             131..1639
                     /locus_tag="PK576_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOE61996.1"
                     /translation="MDGKKVFAVDLGASGGKCFVGVFSDKGFRMEEIHRFAHEGVSCF
                     LPDRSGEVTERTFWDDTFIYQNIVKGLQAARRQCGAVLDGIGIDTWGADGNLVTRDGD
                     LLGKVYCYRDHRLDAMCEEVKARLDATKVYEITGNHFQPFNQSNQLLWLATRRPELLK
                     LAEVYLPIPSLFYYYLGGCKVVDSTFASVTQMMDAKRGKWSREMLKALGIPKRLMPEI
                     VKPGTKVGRLQPKLAAACGLNEADLIAVGSHDTASAFAAAPVRNVKTALIISSGTWSL
                     VGKLIRKPITTPEAMAAGLSNEGGIGNTRFLRNCMGTWIVQELLRVWEIADGKRMPWS
                     EVDTLTPAAPAFTAFIDPDDSRFYNPANMEQEIRAYLNETGQPAPVDRGTVLRCVYES
                     LALKYRQVNETINRVTGTDTEAVHIVGGGSNNALLNQFTADSVGVPVFAGPKEATAVG
                     NLMVQAVGAGILPDLKAAKSIIRASFPITVFQPQQTDAWDAAYARFSSFVHY"
     gene            complement(1789..2730)
                     /locus_tag="PK576_10190"
     CDS             complement(1789..2730)
                     /locus_tag="PK576_10190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOE61997.1"
                     /translation="MKPPFVSVIIPTYNRAQTACDCVASVLRSDYRAIEVIIVDDASP
                     DNTRELVERQFGNDPRVRYVRNEKNLQAAAVRNRGASLAKGENLLFVDDDNLVEPDMV
                     SKLVHGMSLDSGLGQIAPLAIHKAPNLIWTLGSTFNLWTSQPKNLNEREPADRVALED
                     RLYPSWYSPNVWMVKKEAFDRIGGYDPFYRIMFDESDFGMRLRRAGYRSAIYPMARTY
                     HLGFIEPGKTAALRCLGIESPERAYCFARNRSVFVRRHFPPVNRAVVLTVFVPLFAVY
                     YGAVALRHRRPDIAWAFFKGSFVGWFSFPKEPQGTDA"
     gene            complement(2755..3717)
                     /locus_tag="PK576_10195"
     CDS             complement(2755..3717)
                     /locus_tag="PK576_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE61998.1"
                     /translation="MTILWLAGNLLHTGWVETRRFFWERRVTRDPEGLLPEAAAFQVG
                     EGPIALLFIHGFADTPRVWRRFADRLAATGDFTCRALRLAGSAEPAAQAKRASLELWR
                     MQLSDELIRLGEHHKRIWVAGHSMGGALALDTALRQPEAVEGVAALAPLIEVSRARAP
                     VLAPEVWFALARVALCLSPTFESPFSTQAVAADDPDFTYTRDRFIPFSVYQSLFRLAR
                     SNRKALGRLTQPVFAVTANRDVVVDTSAALRWLAECRGPKRILDLQDVGHVIPLENGW
                     RAVADDLAAFIRDHTRQEGRRLPEASADSSGELSLPESEETPPA"
BASE COUNT          778 a         1146 c         1216 g          709 t
ORIGIN      
        1 cggtatactc acacacaaag gtcagccaca cgcgggaagc gggctgaagc gacgggaagt
       61 tcacgggcag gaggtcccgc gcgttttcag gtgaagacgc ggaaacaaag agggtgttga
      121 ggaggtcagc atggacggca aaaaagtgtt tgcggtggat ttgggtgcgt caggcggaaa
      181 atgctttgtc ggcgtgtttt cggacaaggg tttccggatg gaagagatac accggttcgc
      241 gcatgagggc gtgtcctgtt ttctgccgga ccgttctggc gaggtcacag agcgtacatt
      301 ttgggacgac acctttatct accagaatat cgttaaaggt cttcaggctg cgcggcgcca
      361 gtgcggtgcc gtgctggacg gcatcggaat cgacacttgg ggcgcggatg gcaatctggt
      421 gacgcgggac ggcgatctgc tcggcaaggt ctattgttac cgtgaccacc ggctcgatgc
      481 catgtgtgag gaggtcaaag cccgtttgga tgccacaaag gtttatgaaa tcacaggcaa
      541 ccatttccag ccgttcaacc agtcgaacca gttgctctgg cttgccacgc gtcggcctga
      601 actgctcaag ctggccgagg tttatctgcc catcccgtcg ctcttctatt attatctggg
      661 cggatgcaag gtggtggact ccacctttgc cagtgtcacg cagatgatgg acgccaaacg
      721 gggcaaatgg agccgcgaga tgctcaaggc gttgggtatc cctaagcggc tgatgcccga
      781 gatcgtcaag cccggcacga aggtcggtcg gctccaaccc aagctggccg cagcctgcgg
      841 tctgaacgag gcggatctga tcgcggtcgg ttcgcacgat accgccagtg cctttgcggc
      901 cgcgccggtg cgcaacgtca agacggcgtt gatcatcagt tccggcacct ggtctctggt
      961 cgggaaactc atccggaagc ccatcaccac gccggaggcc atggccgcag gtctgagcaa
     1021 cgagggcggc atcggcaaca cgcgttttct gcgtaactgc atgggcacct ggattgtgca
     1081 ggaactgctg cgcgtgtggg agatcgccga cggcaagcgc atgccgtgga gcgaggtgga
     1141 cacgctcacg ccggccgcgc cggccttcac ggcgttcatc gatcccgatg attcgcgttt
     1201 ctacaatccg gccaacatgg agcaagagat ccgcgcctac ctgaacgaga cgggacagcc
     1261 cgcgccggtt gaccgcggca cggtgctgcg ttgcgtgtac gaaagtctgg cgctcaaata
     1321 ccgtcaggtc aacgagacga tcaaccgtgt caccggcacg gataccgagg cggtgcatat
     1381 cgtgggcggc ggcagcaaca atgcgctact caaccaattc accgctgatt ccgtcggggt
     1441 gccggttttc gccggaccga aagaggccac ggcggtcggc aacctgatgg tgcaggcggt
     1501 tggcgcgggc atcctgcccg acctcaaagc cgcgaagtcg atcatccggg cttcttttcc
     1561 catcacggtt ttccaacccc agcagaccga tgcgtgggat gcggcctatg cgcgctttag
     1621 ttcattcgtg cattattagt cgggatcggg atcgctatcg ggatcgattg tttcaccaca
     1681 gaacacaacc gcctcaccgc cttgcagcct tttggtctga tagcgataaa accggcaatc
     1741 cccccgtccc cctctctggg tcggggactc tgtccgcatt ggggatcatc aggcgtccgt
     1801 accctgcggt tccttgggga aggagaacca gccgacgaag ctccctttga aaaacgccca
     1861 ggcaatgtcc gggcggcggt gccgcaaggc cacggccccg taatagacgg cgaacaacgg
     1921 aacaaaaacc gtgaggacaa cagcgcggtt cacgggcggg aaatgccgac ggacaaaaac
     1981 ggagcggttg cgcgcgaaac agtaagcgcg ctcagggctc tcgatgccga ggcaccgcag
     2041 ggccgcggtt ttgccgggct cgatgaatcc gaggtggtag gtgcgggcca tggggtaaat
     2101 ggcagaccgg tacccggcac gccggagccg catgccgaag tcgctttcgt cgaacatgat
     2161 ccggtaaaag ggatcatatc ccccgatccg gtcgaaggcc tcctttttga ccatccacac
     2221 attcgggctg taccaggagg gatagagccg gtcttccagc gcgacgcggt ccgcaggctc
     2281 tcgctcgttg aggttcttcg gctgggaggt ccacaggttg aaggtcgagc ccagcgtcca
     2341 aatcaggttc ggcgccttgt ggatcgcgag cggcgcaatc tgccccaacc cgctgtccaa
     2401 tgacatgccg tgaaccagtt tggaaaccat gtcgggttcc acgaggttgt cgtcgtcaac
     2461 gaaaagaagg ttctctccct ttgccagaga cgcgccccgg tttcggactg cggcggcctg
     2521 aaggtttttc tcgttgcgga cgtaccggac gcgcggatcg ttgccgaatt gacgttcaac
     2581 gagttcgcgg gtgttatccg gtgaggcgtc atccacgatg atgacttcga tcgcgcggta
     2641 atcgctcctc aaaacagagg ccacgcaatc gcacgcggtc tgcgcgcgat tgtatgttgg
     2701 gatgatgacg gatacaaagg gtggtttcat acgaaaaggt tctcctgtcg ccgattaagc
     2761 ggggggcgtc tcctccgatt ctggtaggga cagctccccc gaactgtccg cggacgcctc
     2821 ggggaggcgt ctcccctcct gccgcgtgtg atcccggatg aaggcggcga gatcgtccgc
     2881 gacagcacgc cagccgttct ccagcgggat cacgtggccg acatcctgca ggtcgagaat
     2941 ccgtttcggg ccgcggcact cggccagcca ccgcagcgca gccgatgtgt ccaccacaac
     3001 atcccgattg gcggttacgg cgaacacggg ttgcgtcaag cggcccaagg ccttgcggtt
     3061 ggagcgcgcc agccggaaca ggctttggta cacggaaaac gggatgaagc ggtcacgcgt
     3121 gtaggtaaaa tcgggatcgt ccgccgccac ggcctgcgtg gagaagggcg attcaaacgt
     3181 gggcgagagg caaagtgcca cacgggcgag ggcgaaccac acctcgggtg ccaacacggg
     3241 tgcgcgggca cgtgaaacct cgatgagcgg ggcgagcgcc gccacgcctt ccaccgcttc
     3301 cggctggcgc agggcggtgt ccagcgcgag cgcgccgccc atcgaatgcc ccgccaccca
     3361 aatacgctta tgatgttcgc ccagccggat cagctcatcg gaaagttgca tccgccagag
     3421 ttcaagtgac gcgcgcttcg cctgtgcggc gggctccgcg ctccccgcca ggcgcaacgc
     3481 gcggcaggtg aagtcacccg ttgcggcgag ccgatccgcg aaccgccgcc agacacgggg
     3541 cgtatctgcg aacccgtgga tgaaaagcag ggcgatcgga ccctccccga cctgaaacgc
     3601 cgccgcctcg ggcaggagcc cttccggatc acgcgtgacg cggcgttccc aaaagaagcg
     3661 ccgtgtttcc acccatccgg tgtggagcag atttcccgcc agccagagaa ttgtcaaaca
     3721 agccaacccg tataacatat ccaaacaata ctatacccgc gttctccgca caaggcggct
     3781 gaacaaatta gcctaaaaga gcagttgaac cttcaactct ctttggtttc agggtttcag
     3841 ggtttcagg
//
LOCUS       DAOSLE010000208         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_20998_length_3825_cov_7.710345, whole genome shotgun sequence.
ACCESSION   DAOSLE010000208 DAOSLE010000000
VERSION     DAOSLE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20998_length_3825_cov_7.710345"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK576_10200"
     CDS             complement(<1..367)
                     /locus_tag="PK576_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE61999.1"
                     /translation="MDIHADSYRDGDNSNSGLRMKLSGTREVWVRLAGLAVVLGWGAS
                     LRAATQDGALRVEVNSGYNLIVASNAGSSSSYVPGAAYIGVTLHNDGTTDLTDIVVCA
                     GNYNGGTGSTPGIYPSRTHD"
     gene            806..1384
                     /locus_tag="PK576_10205"
     CDS             806..1384
                     /locus_tag="PK576_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOE62000.1"
                     /translation="MASIKGTQTEKLVLTAFTGESAARNRYTFWAGQAKKEGFVQIEA
                     IFEETANQEKEHAKRLFKFLEGGDVPIEITLPAGVIGKTEENLIHAAEGEEYEWQHMY
                     PDFAAVARKEGFEEIAKTMEAIAIAEKFHGERYRQLAANIKKGIVFKREDPNTIWRCR
                     NCGYLHVGAEALDLCPACAHPKAHFELRGINW"
     gene            1432..1797
                     /locus_tag="PK576_10210"
     CDS             1432..1797
                     /locus_tag="PK576_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOE62001.1"
                     /translation="MALASVFEEDLEESFILGSGPGGQKINKTASTVRLLHRPSRLTV
                     KCGVSRSREQNRWLARRELAERILERERSELSIRRQAAEKIRRQKRRRSRRQQARMLD
                     DKRKHSEKKALRGAVRFDA"
     gene            1836..3647
                     /gene="recJ"
                     /locus_tag="PK576_10215"
     CDS             1836..3647
                     /gene="recJ"
                     /locus_tag="PK576_10215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOE62002.1"
                     /translation="MFPKYRWRDSPVEDGSARELADALELPLPLARVLAARGHTSPDQ
                     AAAFLAPNLQAHLGHPDGFPGAAEAAERLWAAVRAGRRIVVYGDFDADGVAAAAVMVS
                     SLKRFGGRVSVFLPHRDPEGYGLTLAALERCRREQGGWPDLLVTVDCGITSINEVEHL
                     NAAGVEVILTDHHEPGTVLPHAAVIVNPRVGASPGAEHLCGAGVAFKVIHAMAELGKR
                     RNWYPGEPFCGELLAPVGVATVADVVPLTGENRLLVSAALRSWERYAGAGLRALLARA
                     SAQTVRDMDAALFGFVLGPRLNAAGRMGSAMDAYELLATCDRQRATELAARLEGFNGE
                     RRGVEARMLAAARHQCGLDSPQGLGDLAAVVVGGDGPHAGATGWHPGVLGIVAARLSE
                     AAARPAAVVAFDETGAGRGSVRAGEGYHALKALAAAGDALTGFGGHERAAGFQVRAGG
                     FAQFRQRFCEACADQARTADGGPCLMVDGWLTPESLSVAFLAAQQRLAPFGYGNPIPR
                     WGLRGVSLERAQPVGNSGEHLQLAFDVGAGRSVRGVWFRHGAMSEAVRAAGGRMDVVF
                     EMRRNDFGGEASVEMRICDLAPTVAEKEVRGRLKKSS"
BASE COUNT          688 a         1079 c         1336 g          722 t
ORIGIN      
        1 catcatgcgt ccgcgaggga tagatacccg gcgtggagcc ggtcccgcca ttgtagttgc
       61 cggcgcacac cacgatgtcg gtcaggtcgg ttgtgccgtc gttgtgcagc gtcacgccga
      121 tgtaggccgc gcccggcaca taggacgagg acgagcccgc attcgaggca acgatcagat
      181 tgtagccgct gttgacctcg acccgcagcg caccatcctg tgtggccgcg cggagggatg
      241 cgccccaccc cagcaccacg gcaagccccg ccagccgcac ccagacttcc ctcgttccgc
      301 tgagcttcat tcttaaaccg ctgttggaat tgtcgccgtc ccgataagag tctgcgtgaa
      361 tatccacgtt tcaccccttc gctgataggt ctgacaggtc aagctcggtt tgacaaaatt
      421 gcgctgaaca tactccttta atcgggtgtt tgcagcgaca ccccctgcca tttacttttt
      481 tatgatacaa aaacaggtgt gtaaggtcta taaaaaagtg tgaggatttt gaatttttgt
      541 gtgaaatccc gtcagcctct ccgcgctatg ctcggagcca aaattcgcgt cttggccgat
      601 aaggccaata aaaacaagtt gtttatcgcg gcaggggcgc ccgataacgg acgcccctgc
      661 cgcgataata ggcagatgaa tcgggaatgg actcttgcgg agaccgttta cgtgattgcc
      721 cggaattacg gttttctgat aagatcatcc cgttttcaaa gaacacgatt caagtccagc
      781 catgagtcaa aaggaggaat gcaagatggc atcgatcaaa ggcacgcaga cggagaaact
      841 ggttctgacc gctttcacgg gggaatcagc agcgcgaaac cgttacacgt tttgggcggg
      901 gcaggccaag aaggaagggt tcgtccaaat tgaggcgatt tttgaggaga ccgcgaatca
      961 ggaaaaagag catgccaaac ggctcttcaa atttttggag ggcggcgacg tgcccatcga
     1021 gatcacactg cccgcaggcg tgatcggcaa gaccgaggag aacctgatcc atgcggccga
     1081 gggcgaggag tacgagtggc agcacatgta tcccgatttc gccgccgtgg cgcgcaaaga
     1141 ggggttcgag gaaatcgcca agaccatgga ggcgatcgcg attgcggaga agttccacgg
     1201 cgagcgttac cgccaactgg ccgccaacat caagaagggt atcgtcttca aacgcgaaga
     1261 ccccaacacg atctggcgtt gccgcaactg cgggtatctg catgtcggtg cggaagccct
     1321 ggacctctgc cccgcctgcg cccatcccaa ggcgcatttc gagttgcgcg gcatcaactg
     1381 gtaagggatg ccctgatcac ccccggaacc atcgagaaga tccgtgcgcg catggctctg
     1441 gccagtgtgt tcgaggagga tttagaggaa tccttcattc tcggatcggg ccccgggggc
     1501 cagaagatca acaagaccgc ctccacggtg cggcttctgc accgccccag ccgactcacg
     1561 gtcaaatgcg gcgtcagccg gtcgcgtgag cagaaccgct ggctggcgcg gcgtgaactg
     1621 gctgaacgta ttttggagcg cgagcgttcg gaactctcga ttcgacggca ggcggcagag
     1681 aagatccgac gccagaaacg ccgacgctcg cggcgtcagc aggcccgcat gctggacgac
     1741 aagcgtaaac acagtgaaaa gaaggcgttg cggggcgccg tgcggttcga cgcctgaacg
     1801 ccgcaggggc cgtttcgggc gaggcggggc gcggcatgtt tccgaaatac agatggcggg
     1861 attcgcccgt agaggacggg tccgcccgcg aattggctga cgcgttggag ttgccgttgc
     1921 cgctggcacg ggttttggcg gcgcgcggcc acacctcgcc ggatcaggcc gccgcgtttc
     1981 tggcgccaaa cttgcaggcg catctggggc atccggacgg gtttccgggt gctgcggaag
     2041 ctgcggaacg gctctgggcc gcagtccggg ctggccgcag gattgtggtc tacggggatt
     2101 tcgatgcgga cggcgtggcg gccgcggcgg tgatggtttc ctcgctcaag cggttcggag
     2161 gtcgggtttc ggtctttctg cctcaccgcg atccggaggg ctacggcctg accctcgccg
     2221 ccttggagcg ctgccgccgc gaacaggggg gatggccgga cctgctggtc accgtggatt
     2281 gcggaatcac ctcgatcaac gaggtggagc acctgaacgc cgcaggggtt gaggtgattc
     2341 tgaccgacca ccacgaaccc ggcacggtat tgccccatgc cgcagtgatc gtgaatccgc
     2401 gtgtcggggc ttcgccgggt gcggagcacc tgtgcggcgc aggcgtggct ttcaaggtga
     2461 ttcacgcgat ggccgaactg ggtaaacgcc gcaactggta tccgggcgaa cccttctgcg
     2521 gcgaactgct ggcaccggtc ggggtggcga cggtcgcgga cgtggtgcca ttgaccgggg
     2581 agaaccggct gttggtttcg gccgcgctcc gttcgtggga gcggtacgcg ggtgcgggat
     2641 tgcgcgcgtt gctggcgcgc gcgtcggcgc agaccgtccg cgacatggat gcggccctgt
     2701 tcggatttgt gctggggccg cggctcaatg cggccggccg tatggggtcg gctatggatg
     2761 cgtacgagtt gttggccacg tgtgaccgtc agcgcgcgac ggagttggcg gcgcgtctcg
     2821 aagggttcaa cggcgagcgg cgcggcgtgg aggcgcgtat gctggccgcc gcgcggcacc
     2881 aatgcggact tgattcgccg cagggactgg gcgacctcgc ggcggtggtg gtcggcggcg
     2941 acggtccgca tgccggggca accggctggc accccggagt actcgggatc gtggcggcgc
     3001 ggctgagcga agcggcggcg cggcccgcag cggtggtggc gttcgacgag acgggcgcgg
     3061 gtcggggttc ggtgcgcgcg ggcgaggggt atcatgcgct caaggctctg gccgcggccg
     3121 gggatgcgct gaccgggttc ggcggacacg agcgggccgc agggtttcag gtacgggccg
     3181 gcgggttcgc gcagttccgg cagcggtttt gtgaggcgtg cgcggatcag gcgagaacag
     3241 ccgacggcgg gccatgcctg atggtggacg gatggctcac gcccgagtcg ctctccgtcg
     3301 cgttccttgc cgcgcaacag cgtctggcac cgttcggcta cggcaacccg ataccgcgct
     3361 ggggtctgcg cggggtgagc ttggaacgcg cgcaaccggt cggcaactcc ggcgagcatt
     3421 tgcaactcgc gtttgatgtg ggcgcgggcc gctccgtgcg gggcgtatgg ttccgccacg
     3481 gcgcgatgtc cgaggccgtg cgcgccgcag gcgggcggat ggatgtggtg tttgagatgc
     3541 gccgcaacga ttttggcggg gaagcgtcgg tggaaatgcg aatctgtgac ctcgcgccga
     3601 cagtcgcgga aaaggaggtc cgcggacgcc ttaaaaagag cagttgaacc ttcaactctc
     3661 tttggtttca gggtttcagg gtttcagggt ttcaaggttt catcggggtc ggggtcggaa
     3721 tcgggatcgg gatcgattgt ttaaccacag gacactttct tgggtttcag ggtttcaggg
     3781 tttctggttt catggtttca gggtttcatc caaatcgggg tcggg
//
LOCUS       DAOSLE010000209         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_21718_length_3725_cov_7.555041, whole genome shotgun sequence.
ACCESSION   DAOSLE010000209 DAOSLE010000000
VERSION     DAOSLE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21718_length_3725_cov_7.555041"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..719)
                     /locus_tag="PK576_10220"
     CDS             complement(261..719)
                     /locus_tag="PK576_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62003.1"
                     /translation="MNQLCFAACVALMLGLSVRAEEKPAAKQEGFPKRANSRAGLIQK
                     FDKDGDGKLNEEERAAMREEMRKNREKAFDKNGDGKLDDTERAAMQEARKAREKAFDK
                     DGDGKLSIEERKAMREELMKNRRGGGPGKGRPDKGKKKGPPPAAPAAPGA"
     gene            complement(827..2092)
                     /locus_tag="PK576_10225"
     CDS             complement(827..2092)
                     /locus_tag="PK576_10225"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOE62004.1"
                     /translation="MPEKTLPLSPAEMERLTQRYPTPFHLYDERGIRENARRLQAAFA
                     WNPGFREYFAVKACPNPFLMAILSEEGFGMDCSSLPELLLSERVGVTGEKIMFTSNNT
                     PAEEFVKARELGAIINLDDLAHIPFLEASAGVPERICLRYNPGPLKGGNAIIGRPEQA
                     KYGFTREQLFEGYRQLKAKGVRHFGLHTMVASNELNPQYFIDTAEILFDLVCELSREI
                     DVPFEFVNIGGGIGIPYRPEQEAVSLETVGEGIRRAYASRLLAKGHPELNLYMECGRM
                     ITGPYGYLVTRVRHIKRTYKTYAGLDACMANLMRPALYGAYHHISVPAKAGAPCDTVY
                     DVTGSLCENNDKFAIDRPLPVLEPGDLVVIHDAGAHGHAMGFQYNGKLRSAEVLLRED
                     GGTLLIRRAETPEDYFATLDFEALKGFRA"
     gene            complement(2143..2219)
                     /locus_tag="PK576_10230"
     tRNA            complement(2143..2219)
                     /locus_tag="PK576_10230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2183..2185),aa:Arg,seq:cct)
     gene            complement(2417..>2633)
                     /locus_tag="PK576_10235"
     CDS             complement(2417..>2633)
                     /locus_tag="PK576_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62005.1"
                     /translation="LSVSPKIGILMVDGKVVDAGLVEIEPGVNHKVEAVCQGYKRIEQ
                     YYKAKPGETRKIDILLEKEVKRSFFGF"
     assembly_gap    2634..2643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2644..>3725)
                     /locus_tag="PK576_10240"
     CDS             complement(<2644..>3725)
                     /locus_tag="PK576_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011102388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62006.1"
                     /translation="APAPEVAAAKPAAVAQPAKPSEPARQPEAKPAPAPEVAAAKPAA
                     VAQPAEPSEPARQPEAKPAPVEKPVTAQAAPSARPAEPAAPVAQKSDPAALRAKEAAE
                     AATRVEGAAARLRKEGLAACASVLDTLAAVPIPLRAEETLKTKWVAVRDASAELLERE
                     ILRKDPLAQRAQRLKTAGEIVQGRAIEAVMEASRVAELSKSLQQQQGVCVLRLVNQGT
                     QTVQVSAREGLEQTSLAGGEVKDWQIPVSGETLTMSITVDEGNRFRSRMETVRLPRGG
                     GVERAIGSAVAQTAAPTPKPEAAPAAKPEPVTPSPPATQPVAAPSPVVAAEKTAPAAP
                     SPAAVAEKTPQTAPAAPAPAAAKAFF"
BASE COUNT          645 a         1243 c         1106 g          721 t
ORIGIN      
        1 ttgtgatcgt gatcgcggcg cagacgctct cgcccgcctc cacgccgtcg cgcccggtgg
       61 tgctcgcgga tcaacgcgcc gccgtcaccg tgatccgtga cgccttcacc ggccgctgga
      121 ccatccacga ttggcgctgg ctcacggaat gatgccgaaa acgcggcggg gaagaaagag
      181 acggcccgtt acagaaaaac ccggctgcgg aggctaaacc ttcgcaaacc gggctcatgt
      241 tcctctctga gcttacgcgt ttacgcgccg ggcgcggcgg gcgcggcggg cggcggaccc
      301 tttttcttac ccttgtcggg cctgcctttt ccggggccac caccccggcg gttcttcatg
      361 agttcctcgc gcatcgcctt gcgctcctct atgctcagct taccgtcccc gtctttgtcg
      421 aacgccttct cgcgcgcctt gcgggcctct tgcatggcgg ccctttcggt gtcatcgagt
      481 ttgccgtcgc cgttcttatc gaacgccttc tcgcggttct tgcgcatctc ctcgcgcatc
      541 gcagcccgct cctcctcgtt caacttgccg tccccgtcct tatcgaactt ctggatcaag
      601 cccgccctgg aattcgcccg tttggggaac ccctcctgtt tggcagcggg cttctcctcg
      661 gcccgaaccg acagacccag catcagcgcc acacatgcgg cgaaacagag ttgattcata
      721 acatgactcc ttgattacgg ttgtcgtgta caacggacaa cgtctggtcg taaaaaacgg
      781 ccccgtgtcc gctttattgc cggcccgtgc cgattcccca tccggctcag gcccgaaatc
      841 ccttgagagc ctcgaaatcc agcgtggcga agtagtcctc aggcgtttcc gcgcgccgga
      901 tcagaagcgt gccgccatcc tcgcgcaaca gcacctcggc cgaacgcagc ttcccgttgt
      961 attgaaaacc catggcatgc ccgtgcgcgc ccgcgtcatg gatcacgaca agatcccccg
     1021 gctccaaaac cggcagggga cggtcgatcg cgaacttatc gttgttttcg cacagcgacc
     1081 cggtcacgtc gtacaccgtg tcgcacgggg cacccgcttt ggcgggcacg gagatgtgat
     1141 ggtacgcgcc gtagagcgca ggccgcatca ggttcgccat acaggcatca aggccggcat
     1201 aggttttgta ggtccgcttg atgtggcgca cacgcgtgac cagatacccg tacggtccgg
     1261 tgatcatccg gccgcactcc atatagagat tgagttcagg atggcctttg gccagcaggc
     1321 gcgaggcata cgcgcgccgg attccctcac ccacggtttc cagactcacg gcctcctgtt
     1381 cgggccggta cgggatgccg atgccgccgc cgatgttcac gaattcgaac gggacatcga
     1441 tctcgcgaga cagctcgcac accaagtcaa agaggatctc tgcggtatcg atgaaatact
     1501 gggggttaag ctcgttcgac gccaccatcg tgtgcaaccc gaaatgcctc acgcccttgg
     1561 ccttcagttg ccggtacccc tcaaaaagct gttcgcgcgt gaacccgtat ttggcctgct
     1621 cgggacggcc gatgatcgca ttgccgcctt tgagcggacc ggggttgtac cgcaagcaga
     1681 tgcgctccgg aacgcccgcg ctcgcctcca gaaaagggat gtgcgccaga tcgtcgaggt
     1741 tgatgatagc cccgagttcc cgcgccttga cgaactcttc ggcaggcgtg ttgttcgacg
     1801 tgaacatgat cttctcgccc gtaaccccga cgcgttcgga aagcagcaat tccggcaatg
     1861 agctgcaatc catgccgaac ccctcctcgc tcaagatcgc catcaggaac gggttgggac
     1921 aggccttgac cgcaaagtac tcccggaatc cggggttcca ggcgaacgcc gcctgaaggc
     1981 ggcgcgcgtt ttcgcggata ccgcgctcat cgtacagatg aaagggggta ggataacgtt
     2041 gcgtcaggcg ttccatttcg gcaggcgaca gcggcaaagt cttttccggc ataaactgcg
     2101 gctccacacg tcattccgac ggattcaaac aagacagcaa aatggtaggc caggccggag
     2161 tcgaaccggc gacccccaga ttaggaatct agtgctctgt cctactgagc tactggcctt
     2221 taaacggtta gtgataacat aacggggtga tctctggcaa gcccgcgacg cagagcctac
     2281 gcacttccaa cacgggcggt accccacaac caaatttcac ctcacataac ggcacaaagg
     2341 acacaacgtg tttttcatga cgccccattc tccttcatgc cttttgtgtc ttccatgaga
     2401 aacaactttt ttcttatcag aacccgaaga acgaccgctt gacctctttc tccagcagga
     2461 tgtcgatctt gcgcgtttca ccgggcttgg ccttgtaata ctgctcgatc cgcttgtagc
     2521 cctgacacac cgcctcgacc ttgtggttca cgccaggctc gatttcgacc agaccggcat
     2581 ccaccacctt gccgtccacc atcagaatcc cgatcttggg cgacaccgaa agcnnnnnnn
     2641 nnnggaagaa cgctttggcg gccgcaggcg caggagccgc tggcgcggtc tgcggagttt
     2701 tctcggccac ggcagcggga gaaggcgcgg cgggcgcggt tttctcagcc gcaacaacgg
     2761 gggacggcgc ggctaccggt tgagtggcgg gcggggaagg agtgacgggc tcgggcttgg
     2821 cggcaggcgc ggcctcgggt ttgggcgtgg gtgccgccgt ctgcgcgacc gcgctgccga
     2881 tggcgcgctc gaccccgccg ccgcgcggca gccggacggt ctccatacgc gaacggaacc
     2941 ggttgccctc atccaccgtg atcgacatcg tgagggtctc tcccgacacc gggatctgcc
     3001 aatccttgac ctcgccaccg gccagcgacg tctgttccaa cccctcgcgc gccgagacct
     3061 gtaccgtctg cgtgccctga ttgaccagcc ggagcacaca cacgccctgc tgctgttgca
     3121 ggcttttgct cagttccgcc acgcgcgagg cctccatcac ggcctcaatg gcccggccct
     3181 gaacgatctc cccggcggtc ttcagccgct gtgcgcgttg cgcgagcgga tctttccgca
     3241 ggatctcccg ctccaacaat tcggcgctag cgtcccgcac ggcaacccac ttggttttga
     3301 gagtctcctc cgcacgcagc ggaatcggaa ctgcggcgag cgtatccaac acgctcgcgc
     3361 aggctgcgag gccctctttc cggagacgcg ccgccgcacc ttccacccgc gtagccgcct
     3421 cagccgcttc tttggcgcga agcgcggccg gatcggattt ttgggcgacc ggagcggcag
     3481 gctcggcagg ccgggcagaa ggtgcggcct gagctgtaac gggtttctcg accggcgcgg
     3541 gcttcgcttc gggctgccgg gcaggctccg aaggctcggc cggctgcgcg acggcagcgg
     3601 gtttcgcggc ggcaacctcc ggggccggcg cgggcttcgc ttcgggctgc cgggccggtt
     3661 ccgaaggctt ggcgggttgc gcgacggcag cgggtttcgc ggcggcaacc tccggggccg
     3721 gagcg
//
LOCUS       DAOSLE010000210         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_22605_length_3611_cov_5.500281, whole genome shotgun sequence.
ACCESSION   DAOSLE010000210 DAOSLE010000000
VERSION     DAOSLE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22605_length_3611_cov_5.500281"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PK576_10245"
     CDS             <1..1200
                     /locus_tag="PK576_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE62007.1"
                     /translation="WRREQAPLEREAGYVHHLLTDGAYAKEIRVMGHGTFCRNRFTAV
                     RSRLRAARLRLLRLVLTQELAMQFLALATMGVLLLWMAGRLAAGALSLGALVMYAQAV
                     QRGQAQTGALAGTLAELYQSALFLESFDTLLAKPDTVRTPSRPRALPAPGCHGLVFER
                     VSFTYPGTSRPVLREISFTLHPGERIALVGANASGKSTLLKLMCRFYDPTAGRILFEG
                     VDLREVDPVVWRQRIGALFQDFGQYQFSLAENVWIGDPHGSASDARIAETVRRVGLDA
                     LTRTDPRGLDTPLGRWLREGIEPSLGQWQRVAVARATLRDADLLLLDEPASALDDRAQ
                     DEVIQWLQTVTVGRMAVLASHRRRVIEVADRVLVLREGVLVEEGTIPALRARKGEWAS
                     LYERVLS"
     gene            1181..2455
                     /locus_tag="PK576_10250"
     CDS             1181..2455
                     /locus_tag="PK576_10250"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE62008.1"
                     /translation="MSGSFPKLPERTPAQEDLLRVMRAAVNGQVAEIDPLAWPAVYRL
                     AAMHQVETFLYPLIRAWPPAALPPEEMMHFWRTVFLQAAGRYARVAEQARELFSALHA
                     AGIEMIPLKGVWLAERIYMEGGCRTMNDIDLLVPEARFNEACGVIRSIGYVTPDYDVT
                     DRRVKHAHFQKCGEPLALELHRRLFSHPVSAGEAPSTDWVWHGNMEDRLHGIPVQTFE
                     PERQVAYLAHHILGHRMAVPLKVYLDLCLVCERFAAPLSDTAFRHERARWGAVYGISF
                     VLRMADRLLKSEACRMWAAIPEESAELCEALDVAEQIVIGLDAKSIKLTPSLSRFIRS
                     KGLERIRIGLASLFRSAAEMRQIYPRTVRWFGLAGGYLWRLPDLFLRHGSFLSGSPSC
                     RHQTKVAHKGFEDREQLIAWLCARENDKKGHD"
     gene            2448..>3611
                     /locus_tag="PK576_10255"
     CDS             2448..>3611
                     /locus_tag="PK576_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOE62009.1"
                     /translation="MIKKLTLQVRLGTSFRVACAVLGIVFVSSGAPLTEREALDRLSI
                     WINGHPVMGCLAGNAIASTVRFQEGGGSYSVYAVAFSPVGYVVLNSDDRLPLVVAFSA
                     SSGVNLADLPDNAFRAALLTHVANAAETLGGMDTALAPDLSYRRTAPRRAPLSIEQYG
                     PYLATIWNQCNPYNLLCPEDPGGSAYYGYRVPSGCTPAAYAQVLHYHRWPLRGQGSHS
                     YTDSNGSITGTHSVCFSTPFGWAAMQAAYDPWTTTPQPGDVEVADLMYRLGVAADADY
                     ESDGTASSIRTLGERLNTYLFYEPIGYASSQTALLPGLNESLRTGYPCVVAVPGHAIA
                     ADGLLDDNGTVSYHINYGWGGSNNGWWSADNVAGQALAYGCTSLKPMLMAFPAE"
BASE COUNT          626 a         1088 c         1180 g          717 t
ORIGIN      
        1 tggcgtcgcg aacaggcccc gttggagcgc gaagccggtt atgtgcatca tctgctcacg
       61 gatggcgcgt acgccaaaga gatccgggtg atgggacacg gcacgttttg ccgtaaccgg
      121 ttcacggcgg tgcgttcgcg gttgcgtgcg gcgcggttgc gcctgctccg tctggtgttg
      181 acccaggagt tggcgatgca gtttctggct ttggccacaa tgggagtgct actgctttgg
      241 atggctggac gtttggcggc gggagcgctt tccctcgggg cgctggtcat gtacgcgcag
      301 gcggtccagc ggggacaggc gcagaccgga gcgcttgcgg gaacccttgc cgaactctac
      361 cagtccgcac tgtttctcga atcctttgac accctgctcg caaaaccgga caccgtgcgt
      421 acgccgtccc gaccgcgcgc gcttcccgca ccggggtgtc atggcctcgt gtttgagcgt
      481 gtatccttca cctaccccgg aacctcccga ccggtattgc gcgagatcag ttttaccctg
      541 catcccggcg aacggatcgc gctggtgggg gcgaatgcgt ccggcaaatc cacgcttcta
      601 aagctgatgt gccggtttta cgatccgacg gcgggacgga ttctgtttga gggtgtggat
      661 ttacgcgagg tggaccccgt tgtgtggcgt cagcggatcg gcgcgctctt tcaggatttc
      721 gggcagtatc agttctccct ggccgaaaat gtctggatcg gcgatccgca tggcagcgca
      781 tcggacgcac gcattgcgga aaccgtgcgc cgtgtgggct tggatgccct gacccggacg
      841 gatccccggg gactcgacac cccattgggg cgctggttgc gcgaaggaat cgaaccgagc
      901 ctcggacagt ggcagcgtgt cgcggtggcg cgcgccacgt tgcgtgatgc ggatctgctg
      961 ttgctggacg agccggcaag cgcgctggat gaccgtgcgc aggatgaggt gatccaatgg
     1021 ctgcaaacgg tgacggttgg ccggatggcg gttttggcga gtcaccggcg tcgcgtgatc
     1081 gaggtcgccg accgggtgct ggtgctacgg gagggcgtgc tggtggaaga gggaaccatc
     1141 cctgcgctac gggcgcggaa gggcgaatgg gcgagtcttt atgagcgggt cctttcctaa
     1201 gcttcccgaa cggactccgg cgcaggagga cttgttgcgg gtgatgcggg ctgcggtgaa
     1261 cggacaggtc gcggaaatcg accctctggc gtggccggca gtgtatcgtc tggcggcgat
     1321 gcatcaggtg gaaacgttcc tctatcccct catccgtgcc tggcctcctg cggcattgcc
     1381 gccagaggaa atgatgcact tctggcggac ggttttccta caggcggccg ggcgatacgc
     1441 gcgtgtggct gaacaggcgc gcgagttgtt ttcggcactc catgccgcag gcatagagat
     1501 gatcccgctc aaaggcgtct ggctggcgga gcggatctat atggagggcg gctgccgcac
     1561 gatgaatgat attgacctgc ttgtcccgga agcgcggttt aacgaggcat gcggggtcat
     1621 ccggtcgatc gggtatgtga cgcccgatta cgacgtcacg gacaggcggg tgaaacacgc
     1681 gcacttccaa aaatgcggcg agccgctcgc tctggaactc caccggcgtt tgttcagcca
     1741 tccggtttcg gcgggagaag ccccgtcaac ggattgggta tggcacggaa acatggagga
     1801 ccggttgcac gggatcccgg tgcaaacctt tgaaccggaa agacaggtcg catacctcgc
     1861 acaccatatt ctggggcacc ggatggctgt gccgctcaag gtttatctgg acctgtgtct
     1921 ggtttgcgaa cggtttgccg cgccgctctc ggacacggca ttccgtcacg agagggcacg
     1981 gtggggcgcg gtgtacggca tctccttcgt gctacgcatg gccgatcgcc tgctcaagtc
     2041 cgaggcgtgc cggatgtggg ccgcaatccc ggaggagtcc gccgagttgt gtgaggcatt
     2101 ggacgtcgcg gaacaaattg tgatcgggtt ggatgcgaaa agcatcaaac tcaccccttc
     2161 cctctcccgg tttatccgct ccaaaggatt ggaacggatc cggatcggac tggcgagtct
     2221 ctttcggtct gccgcagaaa tgcgacagat ttatccccgt acagtacgtt ggttcggttt
     2281 agcgggcggc tatctctgga gactgcccga tctgttcctt cgccacggtt cgtttctcag
     2341 cggaagcccg tcatgccgac atcagacaaa agtggcccac aaagggtttg aggacaggga
     2401 acagctcatc gcttggctct gtgcgcggga aaacgacaag aaaggccatg attaaaaaac
     2461 tcacattaca ggtgcgtctc ggcacttcct tcagagtggc atgcgccgtg ttgggcattg
     2521 tgttcgtttc atcaggagcc cccttgacgg aacgggaagc gctcgacagg ttatccatct
     2581 ggataaatgg ccatccggtg atgggctgtc tggctggaaa cgccatcgcc tcgacggtca
     2641 gattccagga aggcggcggc tcctacagtg tctatgccgt ggcgttctcg ccggtcggct
     2701 atgtcgtcct caacagcgat gaccggctgc cgctggtcgt cgccttcagc gcctcgtcgg
     2761 gcgtcaatct ggccgacctg cccgataacg ccttccgcgc cgccctgctg acccatgtgg
     2821 caaatgcggc ggagacgctg ggcgggatgg acaccgcgct agcgccggac ctctcctacc
     2881 gccgcaccgc cccccgccgc gcgccattga gcattgagca gtacggccct tatctggcga
     2941 ccatctggaa ccagtgcaac ccgtacaacc tgttgtgccc tgaagatccc ggcggcagcg
     3001 catactacgg ataccgcgtg ccgtccggct gcacgcctgc cgcctacgct caggtgctcc
     3061 attaccaccg ctggccgctc cgcgggcagg gctcgcacag ctacaccgac agcaacggct
     3121 cgatcaccgg cacgcacagc gtctgtttca gcacgccatt cggctgggcg gccatgcagg
     3181 ccgcctacga cccctggacc accacacccc agccgggcga cgttgaagtc gccgacctca
     3241 tgtaccgcct gggtgttgcc gccgatgccg attacgagag tgacggcacc gcctcaagta
     3301 tccggacgct gggcgagcgc ctcaacacat acctcttcta cgagcctatc ggctatgcgt
     3361 cgtcgcagac cgctctgctg ccgggactta acgagagcct gcgcacgggc tatccgtgcg
     3421 tcgtcgcggt gcccggccac gcgatcgcgg cggatgggtt gctggatgac aatggaaccg
     3481 tgtcgtacca tatcaactac ggctggggcg gctcaaacaa cggttggtgg tcggccgaca
     3541 atgtggccgg ccaggcgctc gcatacggct gcacctcgct caagcccatg ctgatggcat
     3601 tcccggccga g
//
LOCUS       DAOSLE010000211         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_22837_length_3580_cov_8.858156, whole genome shotgun sequence.
ACCESSION   DAOSLE010000211 DAOSLE010000000
VERSION     DAOSLE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22837_length_3580_cov_8.858156"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..2216
                     /locus_tag="PK576_10260"
     CDS             225..2216
                     /locus_tag="PK576_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62010.1"
                     /translation="MKRKNTGMARKVRMAVMTVTFIAGVRFVAEAQTVWVSAEDGVWS
                     DAAAWTAGVPDANAAFLTNQTASYKVTVEEPPATHYGNLTLANAAGNTTRLDVAASGF
                     ASTNGMLSIGRGATVAVNTGGVMGYTGRTPATAPFVEIKDGGVWRVDGGTIDFTDLRR
                     VTPTSGSCYVYVGNGSTGRMEVTSGEVLFKGVESSGETNKTVVVRVGNGTGGCGELVM
                     SGGSMTLYNPSSEWSQRVLDIGANGIGVMGAVSLSGEALMLVSNMVYVGGQQGTGTLD
                     VAGNAVFRMPRSNRLYVGKLSKALGIVTVRENGFLNLGGTDGLQMGTDSGRGILNIEG
                     GRAEVCGLHLSVSGSPWAGGYGELNLTGGMLTHRTGNGYGISVARAHTATAGAYGVIR
                     MTGGLIDHSLGLWQQDWSQNGIILGKVGNAANVGGNALGEFFISGGTVTNSGQFILGM
                     GPGATGVVVQTGGDIRQAVVNPSPRSFLTVGWGGGCGSYTLSGGSVVSWRNTYIGGHF
                     TNDYGYVPTDVAFTSNSVGVLRIDGGYLTVTNADLFIGRNGTGTLILGSNGVCTARHV
                     TLTNNTQATVRFELGETGCGTLTAIDTLSLSADAKLEVDTTRYQGEPVWLKLIDCATR
                     KGAFAEEDILVTGKGIVVQDRGDDDVWLFIERGTVIQVF"
     gene            2278..2622
                     /locus_tag="PK576_10265"
     CDS             2278..2622
                     /locus_tag="PK576_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62011.1"
                     /translation="MNLCFVVVNDIDKFEDLLSEFNNRKLYGGTIFDTMGMARAMGQH
                     SGIGHSIRGFLNKGRPFNKTIMMAVSDEQLQAVKEAFETVMGELGSENVGIIVAFPLT
                     AFWGIKDRSSLT"
     gene            2631..>3580
                     /locus_tag="PK576_10270"
     CDS             2631..>3580
                     /locus_tag="PK576_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE62012.1"
                     /translation="MSTLEVLQHIAVVLGASILFSKIFGKFNFPDVTGYLVGGIIIGP
                     MVLGLVPADAVKGLEIISEVALAIIAFSIGSEMRLSALKKTGRGIMLITLLEALGAFL
                     AVFLALWLIFRQEFSFALVLGSIACATAPAATLLVIRQYKAKGPVVDTLIPVVALDDA
                     VCIIAFGICASIAQAIVGGDAVNLLSLVKPVGEIVFSIALGGVYGVIATLLLRHIKTE
                     GELTSFILGSMFLLTSVALRFSLSSLLVMMSFGFALTNLSRAGERAATSLDGLVTPIF
                     MCFFTLSGADLDFGVFATVGFLAIVYVVTRVIGKVAGATAG"
BASE COUNT          689 a          928 c         1178 g          785 t
ORIGIN      
        1 tgtccggtcg ctttttcaaa catgctggcg aaatcattca tggggctctc cgatttcaac
       61 aggatgtctg cacaatacac cctatgcatt taactggcaa gcgcttttcc gctgatttgg
      121 attgcgtttt ttcaatccgt tcgcatacaa tatgcaagga gcttttgagg ggtttcttcc
      181 tgacgggaag aatggttgtg ttcaagccta aaaaggagtc gcagatgaaa cggaaaaata
      241 cggggatggc acgaaaggtc cggatggctg tgatgacggt cacgtttatc gcgggggtgc
      301 ggttcgttgc agaggcgcag accgtatggg tgagtgcgga ggacggggtg tggtcggatg
      361 cggctgcgtg gacagccggt gttccggatg ccaacgcggc gttcctgacc aatcaaaccg
      421 cctcgtacaa ggtcacggtg gaagagccgc ccgcaacgca ttacggcaac ctgaccctcg
      481 ccaatgcggc gggcaatacg acgcggctgg acgtggccgc gtcggggttt gccagcacca
      541 acgggatgct gtcgatcggt cgcggggcaa cggtcgccgt gaacaccgga ggtgtgatgg
      601 ggtatacggg acgcacgccg gcgaccgcac cctttgtcga aatcaaagac ggcggcgtgt
      661 ggcgtgtgga cggcgggacc atagatttca cggatttgcg gcgggtcacg ccgaccagcg
      721 gtagttgtta cgtctatgtc ggtaacggct cgaccgggcg tatggaggtc acgtccggag
      781 aggtcctgtt caaaggggtc gaatcgtctg gcgagaccaa caaaacagtc gttgtgcggg
      841 tggggaatgg aaccggcgga tgcggcgaac tggtcatgtc cggcgggagc atgactctgt
      901 ataatccctc ttccgaatgg agtcagcgtg ttctcgatat cggggccaac gggattgggg
      961 tgatgggcgc ggtctccctg tccggggagg cgctgatgct ggtcagcaac atggtgtatg
     1021 tcgggggcca gcagggaacc ggcacgttgg atgtcgcagg gaacgcggtg ttccggatgc
     1081 cgcggagcaa tcgcctttat gtgggcaagt tgtccaaggc gttagggatc gtgacggtca
     1141 gggagaacgg gtttctcaat ctgggtggaa ccgatgggtt gcaaatggga acggactcgg
     1201 gacgcgggat tctcaatatc gagggcggac gggcggaggt gtgcggattg catctgagcg
     1261 tttcgggatc gccttgggcg ggtggatacg gtgagctcaa tctgacgggc ggcatgctca
     1321 cgcatcggac cggaaacgga tacggaatct cggtcgcccg ggctcatacc gccacagcgg
     1381 gcgcgtacgg tgtgatacgg atgacgggag gcctgattga tcactccctc gggttgtggc
     1441 agcaggactg gtcgcagaac ggtatcattc tcggaaaggt cggaaacgcc gcgaatgtgg
     1501 gcggtaacgc gttgggcgag tttttcatca gcggcggaac ggtgacaaac tcaggccagt
     1561 tcattttggg tatggggccc ggcgcaacgg gtgtggtggt ccagaccgga ggagatatcc
     1621 gacaggcggt tgtgaatcct tcgcctcgat ctttcctgac cgtcgggtgg ggtgggggat
     1681 gtggctcgta cacgctttcg ggcggatccg tcgtgtcttg gcgcaacacc tatatcgggg
     1741 ggcacttcac gaacgactat ggctatgtgc cgaccgatgt cgcgttcacc agcaacagcg
     1801 ttggggtgtt gcgcatcgac ggcggctacc tcacggtcac caatgccgat ctctttatcg
     1861 ggcggaacgg cacgggcacg ctgatcctcg gttcgaacgg cgtctgcacg gcgcggcatg
     1921 tcacgctgac caacaacacg caggcaacgg tgcggttcga actgggtgag accggttgcg
     1981 gtacgctcac ggccatcgac acgttgtcgc tgagcgcgga cgccaaactg gaggtggaca
     2041 ccactcgcta tcagggggag ccggtgtggc tgaaactgat tgactgcgcc acgcgcaagg
     2101 gtgccttcgc ggaggaggac attctggtca caggcaaagg gatcgttgtg caggatcgcg
     2161 gcgatgatga cgtttggctc tttatcgagc ggggcaccgt tattcaggtg ttctagcatc
     2221 ccgaggggat gaaaagaggc aatcccggaa atccgccaac aggagaaagg ctgaagcatg
     2281 aacctctgct ttgtcgttgt caacgacata gacaaatttg aagacctcct aagcgagttc
     2341 aacaaccgga agctgtacgg gggaaccatt ttcgacacca tgggcatggc cagagccatg
     2401 ggacagcaca gcggaatagg ccattccatc agggggttcc tcaacaaggg ccggcctttt
     2461 aacaagacga tcatgatggc cgtttcggac gaacaactcc aagcggtcaa ggaggctttc
     2521 gaaacggtca tgggagaatt gggcagtgaa aatgtgggca tcatcgtcgc ttttcccctt
     2581 acggcatttt ggggaatcaa ggatcgctct tcgctgactt aggaggcgtt gtgtctactt
     2641 tagaagtgct gcaacatatc gcggtcgtcc ttggcgcgtc gatcctgttt tcaaagatat
     2701 tcggcaaatt caacttccct gatgtcacgg gctatttggt cggcggcatc atcatcgggc
     2761 cgatggtgct ggggttggtc ccggctgacg cggtcaaagg actcgagatc atcagcgagg
     2821 tcgcccttgc catcatcgcg ttcagcatcg gttccgagat gaggctttcc gccctcaaaa
     2881 agaccggcag gggcatcatg ttgatcacac tgctcgaagc cttgggcgcg tttttggcgg
     2941 tctttctggc gctctggctg atctttagac aggagttctc gtttgctctg gtgctcggca
     3001 gcattgcttg cgcaaccgcg ccggccgcca cgctgcttgt catccggcag tacaaagcca
     3061 aggggccggt tgtagacaca ctgatccccg tcgtcgcgct ggatgatgcc gtgtgcatca
     3121 ttgctttcgg catctgcgcg tcgatcgccc aggccattgt cgggggcgat gcggtcaacc
     3181 tgctgtctct tgtaaagcct gtcggcgaga tcgtgttttc catcgcgctg ggcggggtct
     3241 atggcgtgat cgctacgctc ctgttgcggc acatcaaaac cgaaggcgaa ctcacgtcgt
     3301 tcatcttagg cagcatgttc ctgctcacct cggttgcgct gcggttttcg ctctcgtcgc
     3361 tgctcgtcat gatgagcttc ggtttcgcgc tgacgaacct cagccgcgcc ggcgaaagag
     3421 cggccacctc gctggacgga ttggtcaccc ccatcttcat gtgcttcttt acgctctcgg
     3481 gtgccgatct tgatttcgga gtcttcgcta cggtcggctt cctcgcgatt gtctatgtcg
     3541 tgacccgcgt catcggcaag gttgccgggg caacagccgg
//
LOCUS       DAOSLE010000212         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_22939_length_3568_cov_5.206092, whole genome shotgun sequence.
ACCESSION   DAOSLE010000212 DAOSLE010000000
VERSION     DAOSLE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22939_length_3568_cov_5.206092"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1373
                     /locus_tag="PK576_10275"
     CDS             261..1373
                     /locus_tag="PK576_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOE62013.1"
                     /translation="MAIYKQKTSRFWWYSISRGRNLPRLCGSTGKEDRNDALAVEAVL
                     RQAHAGTSTRERLVAVIDALTGSRCDGLPLAGVWDVYYGWASRSGKALADITVRQRRR
                     VCVRFAEWAAENWTAVRDAGAVDRACANGFAEFLSKSGAADKTRRNVIADLGTVWEGL
                     RRVRDDVTANPWPLVLPSAESERIAAFTVSEELAVLNAADDAGHGWGLACRIARHTGL
                     RYGDVARLRWDQVDLDKNVIRLRPSKTARHGIGVVLPLCAALHKALSLARPGVSSEFV
                     LPEHAACYPNPWQGKPGRFSEVLKKAGVNGRHTFHSWRHTFRTRLAEAGVSDEIAKRL
                     GGWTVDKTAMRYDHDGRLGELAVAVEAAAKSGGGKN"
     gene            1792..2832
                     /locus_tag="PK576_10280"
     CDS             1792..2832
                     /locus_tag="PK576_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOE62014.1"
                     /translation="MPLCLTQQAPCRIARLVCRAVSTVVSILPFLFVEPAQSSGQPQV
                     PPWAESSFAKTNTPVVPSWLKPKTEKKTVRLPDAPDGWQRLEGCRFVEGKDHDGDSFH
                     VTSGGKNYRFRIYFADCPETDLRDNRYEEQMVWFGVGMDDVLAYGEKAKAFTESALRK
                     PFTVFTKWQNAMGAGNTPRFCGMVYTSEGENLSELLVGAGLARAHGFKCTDFPDRDGW
                     RRFAKGMVEIEQQAKRGQLGIYRKVRSPIVARKSLLPMPSTMPGAGAVLGVNPEAGTG
                     GTHDMGTVMPGVIDINSASKADLMKLPGIGEAFADKIIAARPFASAEDIMRVNGIGPK
                     KYEEIAHRIVVR"
     gene            complement(3037..>3568)
                     /locus_tag="PK576_10285"
     CDS             complement(3037..>3568)
                     /locus_tag="PK576_10285"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOE62015.1"
                     /translation="GGEVWFSDEAGVRSDAHSGTTWAPKGQTPIVSTTGARFGLNVIS
                     AVNRRGALRFMCVEGKVNADIFIEFLKRLVAGAGHPVFLVVDGHPTHKAVKTRKFVES
                     LNGKLHLFFLPGYSPDLNPDELVWNNLKNQGTGKCAITGPDQLKKLVVGHLRSMQKNE
                     ELVRSFYQTPTTKYAA"
BASE COUNT          737 a          893 c         1172 g          766 t
ORIGIN      
        1 gcaggcagca gatagcagac agaacgaact gcgctataaa ttaaacttcc gcttgtgccg
       61 caaagggttg ttcaaaagaa aatggagcgg gcgatgggaa tcgaacccac taattgctag
      121 tccctgcaac atggaaaaag cccattaatc cttgaattta atggtttttt actttctggt
      181 tgtataaacg tagtattgac acagcggctt tttctggtac agttgcgcac ggttgctttt
      241 tgttatgtgt gaggtgagca atggcgattt ataaacagaa gacgtctagg ttttggtggt
      301 actcgatttc tcgcggccgc aacctcccga ggttgtgcgg gtcgaccggg aaggaggacc
      361 gcaacgatgc ccttgccgtt gaggcggtgt tgcggcaggc gcatgccggg acctccacaa
      421 gggaacgtct tgtcgcggtg atcgatgccc tgaccgggag ccgttgtgat ggcttgccgc
      481 tcgccggcgt gtgggatgtt tactacggat gggcgtctcg atcagggaaa gcgttggccg
      541 acatcacggt gcggcagcgg aggcgcgtgt gcgtccgttt cgctgaatgg gcggcggaaa
      601 actggactgc cgttcgtgac gccggggcgg tggaccgggc ttgcgcgaac gggttcgcgg
      661 agttcttgtc gaagagcggg gcggcggata agacacggcg gaacgtgatc gcggatctcg
      721 gcacggtgtg ggaagggctt cgccgtgtgc gggatgacgt aacagccaat ccatggcctt
      781 tggtgttgcc ttcggctgag agcgaacgga ttgctgcatt taccgtctct gaggagttgg
      841 ctgttctgaa cgccgccgat gatgccggtc atggatgggg actcgcctgc cggattgccc
      901 gacacaccgg acttcggtac ggggacgtgg caaggctacg ttgggatcaa gtggatctcg
      961 acaagaacgt gattcgattg cgtccttcca agacggctcg ccatgggatc ggcgtggtcc
     1021 tgccgctttg tgcggcgctg cacaaggctc tttccttggc ccgtccgggc gtgagttctg
     1081 aatttgtttt gccggagcat gcggcgtgtt acccgaaccc gtggcagggc aagccgggcc
     1141 gtttttcgga ggttttgaag aaggccggcg taaatggacg gcatacgttc cattcctggc
     1201 gccacacgtt caggacacgt ttggcagagg ccggcgtcag cgacgagatc gcgaagcggc
     1261 tcggcgggtg gacggtcgac aagactgcga tgcggtacga tcacgacgga cgcctcggcg
     1321 aattggccgt cgccgtggag gccgcggcaa agtcaggagg agggaagaat tgaggggtca
     1381 gcatgactcc ggcgcggcga agcaaaagca gcgacgggaa aaacggatga aggtgttctg
     1441 acacttgcct cgaatgtacc gcccccgttg ttttcgattt ggtcttggac acggcgtgcg
     1501 gcatgggtta aactttagac gtatttcatg aggcggtcta tccgcctttg gtgaaccaaa
     1561 aattttgcgt agcttcggct gctgaccact gcgaaacggg aatttcaaag gagaggcagc
     1621 gctcaaagca cgccccggat tggggcgtgc tgaaagcgtt ctctccaagg cattcccgtg
     1681 cctgcagtgg ggcgtgagta ggcatgcccc tctttttggg cacggttgct attggtggag
     1741 agaaagccgg ccggcggcga ggcggacgcg ataagggaaa gcgtgtctgc tatgccgctg
     1801 tgtttaacgc aacaggcgcc atgccgtatt gcccggttgg tctgccgagc agtttcgaca
     1861 gtcgtttcca tattgccgtt ccttttcgtt gaacctgcac agtcgtcagg acagccgcag
     1921 gtgccgcctt gggccgagtc ctcgtttgcc aagaccaaca ctccggtggt cccttcgtgg
     1981 ctgaagccga aaaccgaaaa gaaaacggtt cgtttgcctg acgcgcctga cggatggcaa
     2041 aggcttgaag gatgccgttt cgtcgagggc aaagatcatg acggagacag ctttcacgtg
     2101 acgtcgggcg ggaagaatta tcgttttcga atctacttcg cggactgtcc cgaaactgac
     2161 ctcagggaca accgctatga ggagcagatg gtgtggttcg gcgttggcat ggacgacgtg
     2221 ctggcgtacg gggagaaggc gaaggcgttt actgaaagtg ctttgagaaa acctttcacg
     2281 gttttcacca aatggcagaa cgcgatgggc gccgggaata caccgcgctt ttgcggcatg
     2341 gtgtacactt cagaggggga gaacctgagc gagttgttgg tgggtgcggg tcttgcccgc
     2401 gcccacggct tcaagtgcac ggattttccc gatagggatg gctggcgccg gtttgccaaa
     2461 gggatggttg aaattgaaca gcaggcaaag aggggacaac tcgggatcta cagaaaggtg
     2521 aggtctccga ttgtggcgag gaaatctctt cttccgatgc cttccacgat gccgggggcg
     2581 ggtgccgttc tgggagtgaa cccggaggca ggcacaggag gcacgcacga catgggcacg
     2641 gttatgccgg gtgtgatcga catcaattcc gcttcgaagg cggatcttat gaaattgccg
     2701 ggcatcggcg aggcgttcgc cgacaagatc atcgcggcaa gaccgttcgc gtccgctgaa
     2761 gacatcatgc gcgtgaacgg catcggcccg aagaaatatg aggagatcgc gcatcggatc
     2821 gtggtgcggt gagaggacgc cgcagttatt gagactacag agaaagcgca ttatggatga
     2881 tgggtaaaaa ggggctggaa cagggaactg ccgcaactgc ggggtgaagg gggctcatga
     2941 aggcgcaaac cgtatccggc ggacacggta gcggtgctca agggcatgct tcaatggggc
     3001 cgcgccgatt actaagcgtc tcaaaataga aacaactcat gctgcatact ttgttgttgg
     3061 tgtctgatag aatgatcgga ccaattcctc gttcttctgc atggaacgca aatgtcccac
     3121 gaccagtttc ttgagctgat caggcccggt gatcgcgcac ttgccagtcc cttggttctt
     3181 gagattgttc cataccagtt cgtcggggtt caggtcaggg gagtacccgg gaagaaagaa
     3241 gaggtgcagt ttgccgttta gagattccac aaacttgcgt gttttcactg ccttgtgggt
     3301 ggggtgccca tcgacgacga ggaagaccgg atgacctgcg ccagcgacga ggcgcttgag
     3361 gaattcgatg aatatgtccg cgttgacctt tccctccaca cacatgaagc ggagtgcgcc
     3421 ccgtctgttg acagcggaga tcacgttcag cccgaaccgg gcacccgtcg tactgacaat
     3481 cggcgtctgc cccttcggcg cccatgtcgt gccgctgtgc gcatccgacc tgacgccggc
     3541 ctcgtctgag aaccacacct cgccgccg
//
LOCUS       DAOSLE010000213         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_23405_length_3506_cov_7.963489, whole genome shotgun sequence.
ACCESSION   DAOSLE010000213 DAOSLE010000000
VERSION     DAOSLE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23405_length_3506_cov_7.963489"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PK576_10290"
     CDS             <1..702
                     /locus_tag="PK576_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF/VirB11 family protein"
                     /protein_id="HOE62016.1"
                     /translation="LHKNVIVSGATSSGKTSVLNVLSGFIPENERILVIEDASELQLQ
                     QTHTVCFETRKPDKNDKGEVTIRDLIHSALRLRPDRLVIGEIRGGEALDLLQALNTGH
                     AGSMSTIHANSSRDSLSRIETCSLLSGIDIPLSALRSQVASAIDVVVHTARLSDGSRK
                     ITAISEVLGLKDGEYVLQDLYRFDVRGIGPDGSLDGVFTGTGQKPTFLHEAALRKLPL
                     DEAMFEKEGNTNAGR"
     gene            753..1118
                     /locus_tag="PK576_10295"
                     /pseudo
     CDS             753..1118
                     /locus_tag="PK576_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
     gene            complement(1278..2216)
                     /locus_tag="PK576_10300"
     CDS             complement(1278..2216)
                     /locus_tag="PK576_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOE62017.1"
                     /translation="MNAARNLLPWFRLFRLPNLLTVPGDPVAGFLLAAGTGLTRNHLL
                     PLLAAAGASLGLYVFGLILNDLLDVATDARERPDRPLPAGEITVPQARMAAIAAALSG
                     LNLAATAGRAALLIAAALAGLIVLYNAVLKRIPVVGVLTMGLCRGLSLMVGAVTACPG
                     LWTQPDVSRLPVLLAAAGLSLYVAAFSVLALREAEPDAVRHPSVRWLPLITLSFTLPT
                     LFAMMAAQGRIGSGIGPAFGVFLMTLALLQAWLTGGPLHSVLPVPELVGRHICNLLRV
                     QACFCAATGIGGILPAVCLTALSFAFVPLAKRFYSS"
     gene            complement(2230..3225)
                     /locus_tag="PK576_10305"
     CDS             complement(2230..3225)
                     /locus_tag="PK576_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOE62018.1"
                     /translation="MRILLTASVLCCVSALSAADACCAAANPYVGRWALTIPGGRAGW
                     LGVEQLTCGALKGSILWGGGSVVPVAETRMENGALLLTRKHNRKKEGKEVQHIETITA
                     RREGNALALLTRTADENGKELSRAEFTGVKIPDLPSRPDLSKLRFGTPVALLARNDLS
                     GWKVMNEKAPNCWSVKDGVLSNRVAGPDGKNRHGTNIRTEAEFEDFNLKTEVRVPKGG
                     NSGIYLRGIYEVQVAETYGRPVNPHNMGALYSRITPAIAAEKPAGEWQTFDITLVDRH
                     LTVILNGKTIIDNQPVLGCTGGAMTSDEFRPGPIYLQGDHTDVDYRNMVITPIVK"
BASE COUNT          706 a         1057 c         1120 g          623 t
ORIGIN      
        1 ctgcacaaga acgtgatcgt gtcgggcgcg acctcctccg gcaagacctc ggtgctgaat
       61 gtgctcagcg gcttcatacc cgaaaacgaa cggattctgg tcattgagga cgcgagtgag
      121 ttgcagttgc aacagacgca cacggtttgc ttcgagacac gtaagccgga caaaaacgac
      181 aagggcgagg tgacgattcg cgatctgatc cactccgcgt tacggttgcg gccggatcgg
      241 ttggttatcg gcgagattcg cggcggcgaa gcgctggatc tgctgcaggc actgaacacg
      301 ggacacgcgg ggagtatgtc gacgatccac gccaacagtt cacgcgacag cctctcgcgt
      361 atcgaaacct gctcgcttct aagtggcatc gatattccgc tctccgccct gcgctcccaa
      421 gtcgcctccg ccatcgacgt ggtggttcac acagcccgcc tgtccgacgg ctcgcgcaaa
      481 atcacggcga tctcggaggt gttgggcctg aaggatggcg agtatgtcct gcaggatctc
      541 taccggtttg acgtgcgggg gatcggtccg gatggcagtc tggacggcgt gttcacgggg
      601 acaggccaaa aaccgacctt cctgcatgag gcggcgttgc gaaagttgcc gctggacgag
      661 gcgatgtttg agaaggaggg gaacacaaac gccggacgct gaacatccaa cgccagacgg
      721 aaaacggagt cacccttcag cgttataaaa aaatggatcg catgacccca gagcagcggc
      781 atcactgcat gtcgcgcatc cgcagcaagg ataccgcgcc ggaatgggca gtacggcgcg
      841 cggttttcgc catggggttc cgttaccggc tgcatgacaa acgacttccg gggaaacccg
      901 atctggttct gccgcgtcac cggaaagtga ttttcgtcca cggctgtttc tggcatgcgc
      961 acgggtgccg gatcggggcg cggatacccg ccacgcaccg gcagttctgg aaaaagaagt
     1021 tcgccaacaa cgtgcggcgt gaccgggagg cgttgcggca gttgtggcgt gacggttggc
     1081 ggaccctggt ggtgtgggag tgcgagaccc atgatcctgt gcggctccgt aaggtgctgg
     1141 cggattttct ggaaccgacg ttccgcgtgc cggattacgg gctggaggaa cgtcacgcgc
     1201 tgtacggcat ggtggccgaa ccgcaggaag aaggccgctc gggttattgt ccggcgcggc
     1261 cagtcacgcg gggcggttca actactgtaa aaccgcttcg ccagcggaac aaacgcgaac
     1321 gatagcgccg taaggcatac ggctggcagt atgccgccga tcccggtcgc ggcacagaaa
     1381 caggcctgta cccgcaacag gttgcagatg tgccgtccca caagttcggg caccggcaag
     1441 accgaatgca acggcccgcc tgtcagccag gcttgaagca gggcaagggt catcagaaaa
     1501 acaccgaaag ccgggccgat gccgcttccg atgcggccct gcgcagccat catcgcgaac
     1561 agggtcggca acgtaaagga cagcgtgatc agaggcagcc agcggacgga agggtggcga
     1621 acggcatccg gctccgcctc gcgcagggcc aagacagaga aggccgccac atagagagac
     1681 agtcctgccg cagccagaag tacgggcaga cgggaaacgt cgggttgcgt ccacaagccg
     1741 ggacaggccg tgaccgcgcc caccatgagg ctcaggcccc ggcagagccc catggtgagg
     1801 acgccgacca cggggatacg tttcaacaca gcgttgtaga gtacgatcag gccggccagt
     1861 gcggcggcaa tcagcaatgc ggcacgtccc gccgttgccg cgaggttgag accggagagc
     1921 gcggcagcga tggctgccat ccgcgcctgc ggcactgtga tctcccccgc aggcaagggg
     1981 cgatccggac gttcgcgcgc gtcggttgcg acgtcgagca gatcgttgag gatcaggccg
     2041 aacacgtaga ggccgagcga tgcgcccgca gcggcgagta gcggaagcag gtggttccgc
     2101 gtcaaacccg ttccggcagc cagcagaaaa ccggccaccg ggtcaccggg gacggtaagc
     2161 aggttgggca accggaacag ccggaaccac ggcaacaaat ttcgggccgc gttcatccgg
     2221 cgcgacgcct cacttcacga tcggagtgat caccatgttc cggtaatcga catccgtgtg
     2281 gtcgccttgc agatagatcg ggccgggccg gaactcatcc gaggtcatgg cgcctccggt
     2341 gcacccgaga accggctggt tgtcgatgat ggtcttcccg ttcagaatca cggtcagatg
     2401 gcggtcaacg agcgtgatat cgaacgtctg ccattcgcca gccggttttt ccgctgcgat
     2461 ggcgggcgtg atacggctgt agagcgcgcc catgttgtgc ggattcacag gccggccata
     2521 ggtttcggcc acctgcacct cataaatacc ccgcagatag atgccgctgt tgccgccctt
     2581 gggcacgcgg acctcggtct tgaggttgaa atcctcaaac tccgcttccg tgcggatgtt
     2641 ggtgccgtga cggttcttcc cgtccggacc cgcgacccgg ttgctcagga cgccgtcctt
     2701 cacgctccag cagttggggg ccttctcgtt catgactttc catcccgaga gatcatttcg
     2761 cgcgagaagc gcgacaggcg tgccgaaacg gagcttggac aggtccgggc gggaaggcag
     2821 gtcggggatc ttgacgccgg tgaattccgc gcgggagagt tccttgccgt tctcatccgc
     2881 cgtgcgggtc agcagcgcca acgcgttgcc ctcgcgccgg gcggtgatcg tttcgatatg
     2941 ctggacctct ttaccctctt ttttccggtt gtgtttgcgg gtgaggagca acgcgccgtt
     3001 ctccatacgg gtttcggcaa ccgggaccac actgccgccg ccccagagga tcgagccttt
     3061 cagcgcgccg catgtgagtt gctcaacgcc cagccaaccg gcacggcccc ccggaatcgt
     3121 cagagcccag cgtccgacat acgggttcgc ggcggcacaa caggcgtcgg cagcggaaag
     3181 agccgaaacg cagcacagga cacttgcggt cagaagaata cgcatccgtg aaacagaaag
     3241 gtttgttttc ataagtgata gtttcccgtt ttcattccgg gaacgcaagt acgaatcgcg
     3301 gttccattga cattccgcac ctctcttcct aaacttcctc tcatccgggc aggtttcgcc
     3361 tcacccccgg caccttcaga aaaagggacg cggcggtgcg cccgacgctc tttttgaact
     3421 ctcagtcccg gcacttcatc gctccacttc atcgcaagcg caatcgactg agttttggag
     3481 accggtgcgt atgcgggact aaggcg
//
LOCUS       DAOSLE010000214         3464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_23772_length_3464_cov_7.198592, whole genome shotgun sequence.
ACCESSION   DAOSLE010000214 DAOSLE010000000
VERSION     DAOSLE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_3464_cov_7.198592"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..3388
                     /locus_tag="PK576_10310"
     CDS             482..3388
                     /locus_tag="PK576_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62019.1"
                     /translation="MLKKVICSCLCVVGAQAEVLTWNGVDGDAWGGAAQNWMDENGAV
                     CAWKNGAEAVFAGSACRVRAIEGVTTSRVRFRSNGFCLEGLAEIPEWIADAGTSNTVA
                     LVDTAPCATVRGGGMIVCAQSARFAALDLQEGLVCLADDSVTTKIHIGAAACLQATKD
                     LAFEELSGTGRLWTGEFPGPEEIPFENDFDSGLSTNKTYTHLLDFGPNPTNAVACLVT
                     FRHVNGPGAAGVSGLPGSSGNLMRMENVGVPRHHGLYSLFSDFAWGFTPEKPIRFKGL
                     EKGRWHRVTIYQRAFEAPGSRGRPAVLSFDMDGDGPLPPASITTWQNLHPVSRITYPF
                     YAVEEDSLTLHIRNLDPARGYHLYGLSIELCDDPRAAQAPIRVTAKKNTFGSAPEQVA
                     ADEAPVLYDLMCDTWAAIDGLGRELPDATACGAPRTDRSVGIFYWTWHRDNLGPYDNT
                     KLTANARDGIVSWPPCTAHWWGESEYGYYRATDEYVLTHHAILLAAAGVDAVFFDTTN
                     PPWTFKEQYEALCRAWTRLREQGTPTPQIAFICPFTFSGRPERAAIVLDQLWYDLYRP
                     GKWKDLWYRWQGKPLVLADPRCAKLPGMKEFFTFRYPEADYRRGPWAADQWSWLEVAP
                     QHVFTNSLGQAEQMSVGVAVNTLPGQPGPAPMSHKDGAMGRSWHDGAIDPDPAAVNRG
                     IFFQNQWDWALKNDPLFIFVTGWNEWRAGRMTKWSRFNAETDCYFPGGLFVDQYNQEY
                     SRDCEPMKGGHGDNYYYQLAANIRRYKGARPVPVATNTYAISVDGAFDDWAAVQPTYR
                     DAIGDVIHRNHRGCGQTHYTDASGRNDIAKLKVAETPQGQVAFYAQTANLLSSHTDPN
                     WMVLFVNTDAQAKTGWHGYDLRVNRMVSADGTSTSLDVWRGEHWECVGVVPIRIGARE
                     IELLLPQKFFGSTPLKFEFHWKDNPESLERHPTIQGDDAPERRFNYVFRQLSDN"
BASE COUNT          711 a          994 c         1063 g          696 t
ORIGIN      
        1 cgataccggt tgccagcccc gcaaacaatg tcagtccgaa agcgatccaa acgtcccgca
       61 tgtcatcctc tcttttccca aaacaccgga gatccgtacc agtccgagta gcttaagcca
      121 gcccgtgacc ggagtcaagc ggacgacgcc atgcaggatg tggcgtcagt gccttatggc
      181 tcgacaagcg cgaaaaaaag ttgtttctca cacgaaggca caaagggcac taaggttttt
      241 tggaagcacc gctcggttgt ttatccacag atggattttt tggggggtag ggctgaccgc
      301 aagtcgcggg tcgtgagtca acctctctta ttgcgaccat gcagtgaccc gcaatcgtga
      361 gagtaaaagc cctgctcggt ttttccacct tacggggggt tgacttcctt catccccccg
      421 cgcacaatgg acagcggctg ttggggtcga aatgtttcct catcttgcag tgagaagcag
      481 catgctgaaa aaggtgattt gtagttgcct gtgcgtggtt ggcgcgcagg ctgaggtgct
      541 gacgtggaac ggtgttgacg gcgacgcatg gggcggcgcg gcgcagaact ggatggacga
      601 aaatggagca gtatgcgctt ggaaaaatgg cgctgaggct gtgttcgccg gttcggcctg
      661 ccgtgtacgc gcgatcgagg gcgtgacgac ctcacgggtg cggttccgtt cgaatggatt
      721 ctgccttgaa ggtctggccg agattcccga atggattgcg gacgcgggaa catccaacac
      781 cgtggcgctc gtggatacgg caccgtgcgc aaccgtgcgc ggcggcggca tgattgtgtg
      841 cgcccaaagt gctcgcttcg ctgcgcttga cctgcaagag ggtcttgtgt gccttgcaga
      901 tgacagtgtg acgacgaaga tacacatcgg agccgccgcc tgcctgcagg cgacgaagga
      961 tcttgcattc gaagaactct caggaacagg acgcctgtgg acgggcgagt ttccgggacc
     1021 ggaggagatc ccgttcgaaa acgattttga tagcggcctc tccacgaata aaacgtatac
     1081 gcatctgctt gatttcgggc cgaaccccac gaacgccgtg gcatgcttgg tgacgttccg
     1141 tcatgtgaac ggtccgggtg cggctggcgt gagcggtctt ccgggaagca gcggaaatct
     1201 gatgcggatg gagaatgtag gtgtgccgcg gcatcatggt ctctattcgc ttttcagtga
     1261 cttcgcgtgg ggattcaccc cggagaagcc gatcagattc aagggcctag agaaagggcg
     1321 ctggcatcgt gtcacgatct accagcgcgc gtttgaagct cccggcagtc gcgggcgccc
     1381 cgccgtgctc tccttcgaca tggacggcga tggtccgctg ccgcccgctt cgatcaccac
     1441 atggcagaac ctccatcccg tctcgcgcat cacgtatccg ttttatgcag tcgaggagga
     1501 ctcgcttacg ttgcacatcc gtaacctcga ccccgcacgc ggctaccatc tctatggact
     1561 cagtattgag ctgtgcgatg atccacgcgc agcgcaggca cccattcgcg tgacggcgaa
     1621 gaagaacacc ttcggatcag ctcctgagca ggtagcagcg gacgaggctc ccgttttgta
     1681 cgacttgatg tgcgatacct gggcagccat tgatggcctc gggcgggaac tacctgatgc
     1741 cacggcatgc ggtgcgccac gcacggaccg cagcgtgggc atcttctact ggacgtggca
     1801 ccgcgacaac cttggcccct acgacaatac gaagctcacg gccaatgccc gcgacggtat
     1861 cgtgagctgg ccgccgtgta cggcgcactg gtggggcgag agcgagtatg gttattaccg
     1921 ggcgaccgac gaatatgtgc tcacgcacca cgccatcctc cttgccgccg ccggcgtgga
     1981 tgcggtgttt ttcgatacca cgaacccgcc atggactttc aaggagcagt atgaggcgct
     2041 gtgccgagcc tggacacgtc tgcgcgagca gggcaccccc actccgcaga ttgctttcat
     2101 ctgtccgttc acattcagcg gccgacccga gcgcgcggca attgtgctgg atcagttgtg
     2161 gtacgatctt taccgtcccg gcaaatggaa ggacctgtgg taccgctggc aggggaagcc
     2221 gctcgtcttg gccgatccgc gctgcgccaa gttgcccggt atgaaggagt tcttcacgtt
     2281 ccgttacccc gaagccgact atcggcgcgg cccttgggct gccgaccagt ggagctggct
     2341 tgaagtcgcg ccgcaacatg tgttcacgaa cagtcttgga caggccgagc agatgagtgt
     2401 gggcgtggcg gtaaacacat tgccggggca acctggcccc gcgccgatgt cccacaaaga
     2461 tggtgcgatg gggcgtagct ggcacgacgg tgcgattgac cccgatccgg cggctgtgaa
     2521 tcgtggtatc tttttccaga accagtggga ctgggcgctg aaaaacgacc cgctcttcat
     2581 tttcgtgacg ggctggaacg agtggcgcgc cggacgcatg acaaagtgga gcagattcaa
     2641 cgccgagaca gactgctact tccccggtgg cctcttcgtg gatcagtaca atcaagaata
     2701 cagccgcgac tgcgaaccca tgaagggggg ccatggcgac aactactact atcagcttgc
     2761 ggccaacatc cgtcgctaca agggggcgcg tcccgtgccc gtcgcaacga acacgtatgc
     2821 catcagcgtg gacggagcgt ttgacgactg ggcggcagtc cagcccacgt accgcgacgc
     2881 cattggcgat gtgatccacc gcaaccacag gggatgtggc cagacccact atacggatgc
     2941 gagcggccgc aacgacattg cgaagttgaa ggttgccgaa acgccgcagg ggcaggtggc
     3001 gttctatgcc cagacggcaa acctcctttc gtcccatacg gatcctaact ggatggttct
     3061 tttcgtgaac accgacgcgc aggcgaagac gggctggcac ggatatgatc tgcgtgtgaa
     3121 ccgcatggtt tccgccgacg gcacgagtac ctcactggac gtgtggcgcg gtgaacactg
     3181 ggaatgcgtg ggcgtcgtgc cgattcgcat tggtgcccgc gagattgaac tgctgctgcc
     3241 gcagaagttt tttggctcca cgccgctcaa attcgagttc cactggaagg acaaccccga
     3301 aagcctcgaa cgtcatccga caatccaagg cgatgatgcc cccgaacgcc ggttcaacta
     3361 tgtctttaga caactaagcg acaactaagg aacaggtaat tcgaaacgct caatgaagac
     3421 cactcgccaa gcttggtttt atcccgttgt tttagcgtat gccg
//
LOCUS       DAOSLE010000215         3449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_23888_length_3449_cov_6.927814, whole genome shotgun sequence.
ACCESSION   DAOSLE010000215 DAOSLE010000000
VERSION     DAOSLE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23888_length_3449_cov_6.927814"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="xseA"
                     /locus_tag="PK576_10315"
     CDS             complement(<1..775)
                     /gene="xseA"
                     /locus_tag="PK576_10315"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE62020.1"
                     /translation="MALPEKRALTVSELTRRIRQTLEDEIGSVWVEGEISNCKRYSSG
                     HTYFVLKDEKAQLNAVLFAGTQALLDPALRLEDGLRVRVFGEITVFESRGQYQLKVRK
                     VEEAGQGDLMRRFEELKRRLQAEGLFEDARKRPLPRLPQRIGVVTSPTGAVIHDILSV
                     LGRRFPNVQVRLAPVKVQGEGAAEEIAEAVAQFNRMCGSGSDWPADLLIVGRGGGSLE
                     DLWAFNEECVVRAVAESVIPVISAVGHNVDYTLCDFAADL"
     gene            complement(786..1571)
                     /locus_tag="PK576_10320"
     CDS             complement(786..1571)
                     /locus_tag="PK576_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOE62021.1"
                     /translation="MKILLVGDIVGGPGRRIFREVVRKLRGEHAVQAVVVNGENAAAG
                     NGITRALAEELFAAGADVITLGDHVWGQKDLEPTIAGEKRLLRPLNFPAGTPGQGSVT
                     VQSTIGSFTVVNLLGRVFMNPMDCPFRAMDAVLKTLPAQIPVLVDFHAEATSEKIAMG
                     HYLDGRVAAVFGTHTHVQTNDAVILPKGTAYITDLGMTGPAVSVIGREIAPVLKKFTT
                     GIPAKFEIARGPCVLEGAILELDRVSGRAVSLTPYRYREPAAG"
     gene            complement(1616..3193)
                     /gene="rny"
                     /locus_tag="PK576_10325"
     CDS             complement(1616..3193)
                     /gene="rny"
                     /locus_tag="PK576_10325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOE62022.1"
                     /translation="MTQFTWYDVSDATVGLLFGLAGVLGGYALRGLIGRWQADAVEKQ
                     AYLKLDEADKEVRNRIKEADIQARTEVVRAREEFEKSTKSRRKELQDIEDRLTFREEN
                     MDKKAALLEKKDQTLAQKQEELQQRDEALRQRKQEADRRYAEAEQRLQKLAGMTHDEA
                     RRDIQQRAEQEVRSEAGGMIRRVQEEARETADREAARIVTMAIQRYALSHASESMTST
                     VPLPSDDIKGRIIGREGRNIRTLEAVTGVNMLIDDTPEAVVISGFDPVRREIARQALE
                     QLVADGRIHPARIEEVVQSVTENMEKTIYEAGEAAAYEAQVQGVPAEILRCIGRLKFR
                     TSFSQNVLRHAIEVAHLMGMMAGELGLDPLIARRIGLFHDIGKALDHDAQGAHALIGA
                     EFLKRNGEDPVVVNGVAAHHEDVPSEGLYGVLCSAADAISSSRPGARVETVGVYVQRL
                     EKLEAIANRFEGVKKTFAVQAGREVRVMVDPGQISDQDAMVMARDISQQISATMQFPG
                     QIKVVVVRESRCVEYAK"
BASE COUNT          560 a         1237 c          950 g          702 t
ORIGIN      
        1 gcaagtccgc cgcaaaatcg cacagcgtgt aatccacatt gtggccgact gccgagatca
       61 ccgggatcac cgattccgcc acggcgcgca ccacacactc ttcgttgaag gcccacaggt
      121 cctccagact gcctccaccc cgcccgacga tcagcaggtc cgccggccag tcgcttccgg
      181 aaccgcacat ccggttgaat tgtgccacgg cttccgcgat ctcttctgcg gcgccctccc
      241 cctgaacctt gaccggcgcg agccgcacct gcacgttcgg gaaacgccgc cccaagacac
      301 tcaggatatc atggatcacc gcgccggtcg gcgaggtcac cacgccgatc cgctgcggca
      361 gacgcggtag cggccgtttc cgcgcgtcct cgaaaagccc ttcggcctgt aaccgtcgtt
      421 tgagttcctc gaaacgccgc atcaggtcgc cctgtcccgc ctcctcgacc ttacggactt
      481 tgagctggta ttggccgcgg ctctcaaaca cggtgatctc gccgaacacg cgcacgcgca
      541 gtccgtcttc cagacgcaac gcgggatcga gcagcgcctg ggtgcccgcg aagagcacgg
      601 cattcaactg cgccttctca tccttcagca cgaagtacgt gtgaccagag ctgtagcgct
      661 tacagttcga gatttcaccc tcgacccaca cgctgccaat ctcgtcctcc agcgtctgcc
      721 ggattcggcg cgtgagttcg gagaccgtca acgctctttt ttcaggcaac gccatgtccg
      781 gttcctcatc ccgccgctgg ctcccgatac cggtacggcg tcagggagac cgccctgccg
      841 cttacccggt cgagttccag aatcgcgccc tccagtacgc agggtccgcg ggcgatctca
      901 aacttcgccg ggatgccggt cgtgaatttt ttcagcaccg gcgcaatctc gcggccgatc
      961 acggaaaccg cggggccggt catccccaga tccgtgatgt aggccgtgcc cttgggcagg
     1021 atcaccgcgt cattggtctg cacatgcgtg tgtgttccga agaccgctgc cacacggcca
     1081 tcgagatagt ggcccatggc gattttctcg gaggtggctt cggcatggaa atccacaagc
     1141 accggtatct gggccggcag tgtcttcagc accgcgtcca tggcgcggaa cgggcagtcc
     1201 atgggattca tgaacacgcg cccgaggagg ttcaccacgg tgaacgagcc gatggtggac
     1261 tgcaccgtca cgcttccctg acctggcgtg ccggctggga aattcagggg ccgcagcagc
     1321 cgcttttcac ccgcgatggt cggttccaga tccttttggc cccacacgtg gtccccgagc
     1381 gtgatcacgt ccgcgccggc cgcgaacaac tcctcggcca gcgcgcgcgt gatgccgttt
     1441 cctgccgccg cgttctctcc gttcaccacc accgcctgaa ccgcatgctc accgcgcagt
     1501 tttcgcacca cctcgcggaa tattcgccgt cccggcccgc ccacgatatc acccaccagc
     1561 agaatcttca tcgcccgccc ctttccgata ttcccgcgct gtccgccgcc tgcggtcact
     1621 tcgcatattc cacacagcgc gattcgcgca ccactaccac tttgatctgt ccggggaact
     1681 gcatcgtggc ggaaatctgc tggctgatgt cgcgcgccat caccatcgcg tcctgatcgc
     1741 tgatctgccc cggatcgacc atcacgcgca cttcgcgccc ggcttgcacg gcgaaggtct
     1801 ttttgacccc ctcgaaccgg ttggcgatgg cctccagctt ttcgagacgc tgcacataca
     1861 cgcccacggt ttcgacccgt gcgccgggcc gcgaactgga gattgcgtcc gccgccgaac
     1921 agagcacgcc gtagagtccc tcggagggca cgtcctcgtg gtgcgcggcc acaccgttga
     1981 ccaccacggg atcctcgccg ttgcgtttca agaactccgc gccgatcaag gcgtgcgcgc
     2041 cctgcgcgtc gtgatccagc gccttgccga tgtcgtggaa gaggccgatg cgccgtgcga
     2101 tcagggggtc cagccccaac tcgcccgcca tcatgcccat caggtgggca acctcgatgg
     2161 cgtgccgcaa cacgttctgc gagaaactcg ttcggaactt gaggcgtccg atacagcgca
     2221 ggatctccgc cggaacgcct tgaacctgag cctcgtacgc tgcggcctcg ccagcctcgt
     2281 aaatcgtctt ctccatattt tcggtcacgc tctgaaccac ctcttcgatg cgcgccggat
     2341 ggatgcgccc gtcggcgacc aactgctcca gcgcttggcg cgcgatctcg cgccgcacgg
     2401 gatcgaaccc ggagatcacc accgcctcgg gtgtgtcgtc gatcagcatg ttgacgccgg
     2461 tcacggcttc cagcgtgcgg atattgcggc cctcgcgccc gatgatgcgt cccttgatgt
     2521 cgtcgctcgg cagcggcacc gtactggtca tggactcgct ggcgtgggag agcgcatagc
     2581 gttgaatcgc catggtgacg atgcgtgccg cctcgcggtc tgcggtttcc cgggcttctt
     2641 cctgcacccg ccggatcatc ccgcccgctt cgctgcgcac ctcctgttcg gcccgttgct
     2701 ggatgtcgcg ccgggcctca tcatgcgtca tgcccgccaa tttctgcagc cgttgctccg
     2761 cctcggcata ccgccggtcc gcttcctgct tacgttgccg caacgcctcg tcccgctgtt
     2821 gcaactcctc ctgcttctgg gcgagcgtct gatccttttt ttcgagcagg gccgccttct
     2881 tgtccatgtt ctcttcgcgg aaggtcaggc gatcctcgat atcctgcaac tccttacgtc
     2941 gggacttggt gcttttctcg aactcctcgc gcgccctcac cacctccgtg cgggcctgga
     3001 tatccgcttc tttgatccgg ttccggacct ccttgtccgc ctcgtccaac ttgagatacg
     3061 cctgtttttc gaccgcatcc gcctgccagc gtccgatcag gccgcgcaag gcatacccgc
     3121 ccagcacacc ggccagaccg aaaagcagac cgaccgtggc gtcactgacg tcgtaccacg
     3181 tgaattgcgt catgaaacct cccaacgcgt taccgcatat gattgtgtca catccgtgtc
     3241 tggacgtccc ggccgaccgg acacaggttc aaatgggaga tatcttatcc gattcccgta
     3301 agcggggcaa cctttgagca agcgaagtcg gaagcccgtc cgttttctcc gcttgatccc
     3361 cgtggaagcc ttgtgtacca ttccgttccg taacgcgtat ccctatcggt tacccgtgcg
     3421 ggacccgatt ttccacgcgt tttccggcc
//
LOCUS       DAOSLE010000216         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_24049_length_3430_cov_7.280000, whole genome shotgun sequence.
ACCESSION   DAOSLE010000216 DAOSLE010000000
VERSION     DAOSLE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24049_length_3430_cov_7.280000"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(401..1618)
                     /locus_tag="PK576_10330"
     CDS             complement(401..1618)
                     /locus_tag="PK576_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE62023.1"
                     /translation="MRDIRVLAQKDHISSLCTASPIQALSELVWNALDADAFDVKIDV
                     IQNPLGGIDAIRVSDDGLGINALEADRHFGNLGGSWKRDTARTPLSGRVLHGSKGRGR
                     FKAFSLGNRVEWRTTFQTADGLRSFVLGGAAADPSLFTLVEAATPGPATGTEVMITEV
                     RNAISSLLDTAWAVQQIASNFALYLKAYPNVRIYFQGLLVNPVIVQHTCRTYHLKSSE
                     GNKAELQVIEWKTKQSRGKVVFCNTDGFALHETDAAVRPGSGFNYTAYLVSQRFADLH
                     EANLLLLEEMHPEVRAFLDAARDILRRHFRERREALTAELLSQWQADGVYPYSGAPAN
                     KAERAARARFEACALAIRAYSDNFDILSSTEKRLLFRLLRETLDARPDSALETVADAL
                     GMPPRQRRALLKS"
     gene            complement(1651..2934)
                     /locus_tag="PK576_10335"
     CDS             complement(1651..2934)
                     /locus_tag="PK576_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOE62024.1"
                     /translation="MCCTPCQMPRALLSALCCWVAAAFAAFAGPDAADWPQFRGPAGQ
                     GIASARNLPRFWSETQNVRWMTRLPGLGHSSPVVAENRVWLTSATDKGRRRHVVCVDL
                     ATGAVLRDRVLYTCDEPEPCHNLNSFATPTPVLEQGRVYVTFGKVGTACLSAETGETL
                     WERRDLPVRYFDVGVASSPILHDDRLILTCDGQADDVRYVIALDKHTGRTLWRTDRTF
                     PDGKIPAYTHSSCIPLVIRVAGREQLISPGPYGVRAYDPASGNELWNVRYGGWSVIPR
                     PVFHNGLLVICNGTVKPLMLCIRPEGAAGDATASAVVWQTSRNVPNMPSPLLLGNRLY
                     TLTASRLSCLEADTGAERWSHTVPGQHMASPVAADGHLYLFNTTGIGSVAALGDTFNL
                     VATNRLTEGCMASPAVVGDSLVIRTRTRLYRIGKP"
     gene            3029..3384
                     /gene="ssrA"
                     /locus_tag="PK576_10340"
     tmRNA           3029..3384
                     /gene="ssrA"
                     /locus_tag="PK576_10340"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          623 a          963 c         1228 g          616 t
ORIGIN      
        1 agggtgttag gctgtgaggc ggtgaggctg ttaggctgtg agggtgtgag ggtgtgaggg
       61 tgttaggctg tgaggctgtt aggctgtgag ggtgtgaggc tgttagactg tgagggtgtg
      121 agggtgttag ggtgtgaggc aatcgatccc gatagcgata ccgatagcga taccgatttg
      181 gatgaaacct tgaaaccaga aacatcgata gcgattccga tagcgatacc gataccgatt
      241 tggatgaaac cctgaaacca tgaaaccgtg aaaccatgaa accgtgaaac cagaaaccca
      301 gaaacctgaa acatcgatag cgatcccgac taataacgca ctaacgcact aatgcactaa
      361 tgcactaatg aactaatgaa ctaacgcacg gttttcaggt tcacgatttg agcagggcgc
      421 ggcgttggcg gggcggcatc ccgagcgcgt cggccacggt ttccagtgcg gagtcggggc
      481 gggcgtcgag ggtttcgcgc agcagacgga agagcaggcg tttctctgtg gaggagagga
      541 tgtcgaaatt gtccgagtag gcgcggatcg cgagcgcgca ggcctcgaaa cgcgcgcggg
      601 ccgcgcgttc ggctttgttg gccggcgcgc cggagtaggg atagacgccg tccgcctgcc
      661 attggctcag cagttcggcg gtcagggctt cgcggcgttc gcggaaatgc cgacgcagga
      721 tgtcgcgcgc cgcgtccaga aaagcgcgca cctccgggtg catctcttcg aggagcagca
      781 ggttcgcctc atgcaggtcg gcgaagcgtt gcgagacgag gtaggcggtg tagttgaatc
      841 cgctgccggg ccgcaccgcc gcgtcggtct cgtgcagcgc gaagccgtcg gtgttgcaga
      901 agaccacctt gccgcggctc tgcttggttt tccactcgat cacttgcagt tcggccttgt
      961 tgccttccga ggacttcagg tggtaggtgc ggcaggtgtg ctggacgatg accggattga
     1021 ccagcaggcc ttggaaatag atgcggacat tggggtaggc cttgagatag agcgcgaagt
     1081 tcgaggcgat ctgctggacg gcccaggcgg tatcgagcag cgagctgatc gcgttgcgga
     1141 cctcggtgat catgacctcc gtgccggtcg ccgggcccgg cgttgcggct tcgacaagcg
     1201 tgaacaggct ggggtctgcc gcggcgccgc ccaacacgaa cgagcgcagg ccgtcggcgg
     1261 tctggaaggt cgtgcgccat tcgacgcggt tgccgaggct gaaggcctta aagcggcccc
     1321 tccccttgct gccgtgcagc acgcgcccgg agagcggcgt gcgcgcggtg tcgcgtttcc
     1381 acgagccgcc gaggtttccg aagtgccgat ccgcctccag ggcgttgatg ccgaggccgt
     1441 catccgacac gcggatggcg tcgatcccgc cgagggggtt ctggatcaca tcgatcttca
     1501 catcgaacgc gtccgcatcc agcgcgttcc agacgagttc gctcagcgcc tggatgggcg
     1561 aggcggtgca cagcgaggag atgtggtcct tttgggccag aacgcgaatg tctctcacaa
     1621 caggatccct ttcttttccg gtcgcgcgcg ttacggtttg ccgatgcggt aaagccgggt
     1681 acgcgtgcgg atgaccaatg aatcgccgac cacggcgggc gaggccatgc acccttcggt
     1741 caggcggttg gtggccacca gattgaacgt gtcgccaagc gcggccaccg aaccgatgcc
     1801 ggtggtgttg aaaaggtaaa gatggccgtc cgcggcaacg ggcgaggcca tgtgctgtcc
     1861 gggtacggtg tgggaccagc gctccgcgcc ggtgtcggcc tcaaggcagc tcagccgcga
     1921 ggcggtgagg gtatagaggc ggttgccgag gagcaggggc gaaggcatgt tcggcacgtt
     1981 gcgggaggtc tgccagacca ccgccgaggc ggtggcgtcg cctgcggccc cttcgggacg
     2041 gatgcagagc atcagcggct tgaccgtgcc gttgcagatg acgagcaggc cgttgtggaa
     2101 gaccggccgg gggatgaccg accagccgcc gtagcgcacg ttccagagct cgttgccgga
     2161 cgcggggtca tacgcgcgca cgccgtaggg gccggggctg atgagctgtt cccgtccggc
     2221 cacgcggatc accagcggta tgcaggacga gtgcgtgtag gccgggatct ttccgtcggg
     2281 gaaggtgcgg tccgtgcgcc agagcgtccg gccggtgtgt ttgtcgagcg cgatcacgta
     2341 acgcacgtca tcggcctgcc cgtcgcaggt gaggatcagg cggtcgtcgt gaaggatcgg
     2401 cgagctggcg acgccgacat cgaaatagcg caccggcagg tcgcgccgct cccagagggt
     2461 ctccccggtc tcggccgaga ggcaggcggt gccgactttt ccgaaggtga catagacgcg
     2521 gccctgctcc aaaaccgggg tgggggttgc gaagctgttg aggttgtggc agggctccgg
     2581 ttcgtcgcag gtgtacagca cgcggtcgcg cagcacggcg ccggtggcga ggtcgacgca
     2641 caccacatgg cgccgccgcc ctttgtcggt ggcgctggtc agccagacgc ggttctccgc
     2701 gaccaccggc gacgagtggc cgaggcccgg caggcgggtc atccagcgca cattttgcgt
     2761 ttcgctccag aagcgcggca ggttgcgggc agaggcgatt ccctgtccgg cgggtccgcg
     2821 gaactggggc cagtcagccg cgtccggtcc ggcaaaggct gcgaaggccg ccgcgaccca
     2881 gcagcagagt gcggaaagaa gggcgcgcgg catctggcaa ggcgtacagc acatacgcga
     2941 actatacgaa aaaaggaggg gtgctgtcca cgcgcgaaaa tccgggttta ctttttgcga
     3001 atctgtgcga tactggtctg cgttttgagg gggcgactgg tttcgacggg gcacgtgaaa
     3061 atccggttgc gcgccgaggg ctcctgttgg cctcgtaaaa aaacaggaaa aaacctaaac
     3121 gcgaacgata acttcgctct cgcggcctaa ttattaggcc cgacgtggaa ccgccgatgt
     3181 ctgctgagcg gggaagcgac gacgagcaga ccctgccggg ggccgagtag ccgggcgccc
     3241 gtgtcttgac gaatcgtggt tgctggtctt cgggtaagcc tgtccgccgg ccttcccgat
     3301 gatgagactg aaagacggga cacgcgcgta gagacgggat ggaagctgtt tcggacggga
     3361 gttcgattct ccccgcctcc accatttttc gcgcttgcgc cgtgacgggg gtttgcgcta
     3421 accttatcca
//
LOCUS       DAOSLE010000217         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_24466_length_3385_cov_8.372973, whole genome shotgun sequence.
ACCESSION   DAOSLE010000217 DAOSLE010000000
VERSION     DAOSLE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24466_length_3385_cov_8.372973"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1242)
                     /locus_tag="PK576_10345"
     CDS             complement(19..1242)
                     /locus_tag="PK576_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE62025.1"
                     /translation="MTLTRRGFFGGSIAVAATSGLKGAVPSPAGKLQGFDESPDAKTG
                     DPWEPFSDKKVRVGIAGYGVCAFGATFSFQDHPNVEVVAAADLFKDRAEMLAKVTGAK
                     RIYLSCEEMVDKEGKNMDAVFITTDAPSHARLSIQALNRGYHVAVAVPAVFGEDQLAL
                     AEELFATVKKTGLVYALFETTAFRPQCFAMRNIYKAGGFGDMVYTEGEYFHFSPPGKP
                     IDSYKGWRIGLPPQYYPTHSNGFYTCVTHKAFTEVTCIGKPSIQPAYQPGGNRYKNPY
                     GSEIALFKTEEGGSARMAVAWDLKGYSGEVGRCYGQLGCFRERYVGDNRLIKDAKLIR
                     TALPPGVENNGHHGGSHPYLTDDFIRAILLKRNPCVDVATALNTTVSGIYAHMSAMKG
                     GETLKIPVYKLLPIN"
     gene            complement(1309..2853)
                     /locus_tag="PK576_10350"
     CDS             complement(1309..2853)
                     /locus_tag="PK576_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOE62026.1"
                     /translation="MTTRRVVCVSVLLSAGVVFGWWDHPYITRCAWDAQPKELTAPYT
                     DFERDEFCYHTMMSDYPRMATAGDFGYFQLWDFFMWWQRRARVDYANGGGEDMSFQRV
                     LQAVKTETPLNALLWAGCFFHALEDSMSISHTALPNEYQGEWHGPCEAFAHWEKFNIG
                     GYVAKPLAGGYDPAALRKELDRRIAEVNKRAYDLWPKMKLIIDENGIKQPPAVKAQTN
                     ERIDPYVMTFAEGVAKLAADTLCTFLNARREFAENTGAALEGAINAPPDPVHAQRTAK
                     VYVRTVDDAPTHFETMAFNGRYAFRNLPDGTYRLAATRLGAKNALSAPFELKKERVTR
                     IDLALETELPQGNLVWNPTGTCDLYGARSPDRWRMKPLFNWGPLQQDYWRTSFMHLEP
                     GWTYRCGAKLKRPTTVRFRFAKSYGGHGPALELDLKDRLESEMVWTNAENFFKAKIEV
                     VSTEPLTNLIEKVWFVPVPPKDWAGIYPMSDYVPVDDIFRQSLRMTPPQPEFWRRDPC
                     ARPPAK"
     gene            complement(2893..3249)
                     /locus_tag="PK576_10355"
     CDS             complement(2893..3249)
                     /locus_tag="PK576_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62027.1"
                     /translation="MNSKEITRYFELRKQLFEIEAQLEALKPAVAEQLRQMNNFVRLD
                     GYDLKLSTYTSYEYSPRVTDLQRTLNETKRKEREEGVAKIKEQRDMLVLKAHRESNVR
                     EEPETYGEWETESGGM"
BASE COUNT          632 a         1024 c          952 g          777 t
ORIGIN      
        1 gaaaatcctg tcatcctgtc aatttatagg caagagtttg tatacgggga tcttgagggt
       61 ctcgccaccc ttcattgcgc tcatgtgcgc gtagatgccg gagaccgtgg tgttgagcgc
      121 cgtggcgaca tccacgcagg ggttgcgctt gagcaggatc gcccggatga agtcgtccgt
      181 aaggtagggg tgcgaaccgc cgtggtggcc gttgttctcc acgccgggcg gcagtgccgt
      241 acggatcagc ttcgcgtctt tgatcagccg gttgtctccc acgtagcgtt cgcggaaaca
      301 gcctagctgt ccgtagcagc gtcccacttc gccggagtat cccttgagat cccaggcgac
      361 ggccatacgc gccgagccgc cttcctctgt tttaaaaagc gcgatctcgc ttccgtaggg
      421 attcttgtaa cgattgcccc cgggctggta tgccggctgg atgctcggct ttccgatgca
      481 ggtcacttcc gtgaatgcct tgtgcgtcac gcaggtgtaa aaaccgtttg aatgcgtggg
      541 gtagtattgc ggcggcaagc cgatacgcca gcccttgtaa gagtcgatgg gttttcccgg
      601 cggagagaaa tggaaatact cgccttccgt atacaccatg tccccaaatc cgcctgcttt
      661 gtaaatgtta cgcatcgcaa agcattgcgg acggaacgcg gtcgtctcga aaagcgcgta
      721 gacgagtccc gttttcttta ccgtcgcgaa aagctcttct gcgagagcga gctgatcctc
      781 gccgaaaacc gccggcacag cgaccgccac gtggtacccg cggttcagcg cctggatcga
      841 gaggcgcgcg tggctcggcg cgtctgtcgt gatgaacacg gcatccatgt tcttcccttc
      901 cttatcgacc atctcctcac aggaaaggta gatgcgtttc gcgcccgtca ccttggcgag
      961 catctcagcc cgatctttga agagatcggc tgcggctacc acttccacgt tcggatggtc
     1021 ttggaaggag aaagtcgccc cgaatgcgca cacgccatag ccggcgatgc ccacgcgcac
     1081 tttcttgtct gagaacggtt cccacgggtc tcccgttttc gcatccggcg actcgtcgaa
     1141 tccttgcagc ttccctgcgg gtgacggtac cgcgcccttg agcccggacg ttgccgcaac
     1201 agcgattgat cccccgaaaa aacctcttcg cgtgagtgtc atcagtccct ccctttcaat
     1261 gaatttacca cagaggtggt acgatagcca ttgcgtattg ctcgaaagtc atttcgccgg
     1321 tggccttgca cacgggtcgc gccgccaaaa ctcgggctgt ggcggtgtca tcctgagaga
     1381 ttggcggaaa atgtcatcca caggcacgta gtcgctcatc gggtagattc ctgcccagtc
     1441 cttcggcggc acgggcacga accagacctt ctcgataaga ttcgtcaacg gctctgttga
     1501 aacgacctca attttcgcct taaaaaagtt ctccgcattc gtccagacca tttcggactc
     1561 aagcctgtcc ttgaggtcga gttcgagcgc cggaccatgt ccgccgtaac ttttggcaaa
     1621 acggaaacgg acggtggtcg gcctcttgag tttcgcgccg caacggtacg tccagcctgg
     1681 ctccaggtgc atgaacgatg tgcgccaata atcctgctgc agcggccccc agttgaagag
     1741 cggcttcatg cgccagcgat cgggagacct ggcgccgtag agatcgcaag tgccagtcgg
     1801 attccagacg aggttgccct gcggaagctc cgtctcgagt gccagatcga ttcgcgtcac
     1861 acgttccttc ttcagctcga acggcgcgga gagcgcgttc ttcgcgccca gacgcgtcgc
     1921 cgcgaggcgg tacgtgccgt ccggcagatt ccgaaaggca tagcgcccgt tgaatgccat
     1981 ggtctcgaaa tgcgttggcg cgtcatccac cgtgcgcaca tacaccttcg ccgtgcgctg
     2041 cgcatggacg ggatcgggcg gcgcgttgat tgcgccctcc agcgccgcgc cagtgttttc
     2101 cgcgaactcg cgtcgcgcgt tcagaaacgt gcacaacgtg tccgcggcga gtttcgcgac
     2161 accctccgcg aaagtcatca catacggatc gatgcgttcg ttcgtctggg ctttgacggc
     2221 cgggggctgt ttgatgccgt tttcgtcgat gataagcttc atcttcggcc agaggtcata
     2281 ggccctcttg ttcacctccg caatccgccg gtccagttcc ttcctgagag ccgccgggtc
     2341 gtatccgccc gcgagcggct tcgcgacata gccgccgatg ttgaacttct cccaatgggc
     2401 aaacgcttcg cacgggccat gccattcccc ctgatattcg ttgggcaggg ctgtgtgcga
     2461 aatggacatc gaatcctcca gcgcatggaa aaagcagccg gcccatagaa gcgcgtttag
     2521 aggcgtctcg gtcttcaccg cctggagaac gcgttggaac gacatgtctt ctccgccgcc
     2581 attcgcgtag tcgacgcggg cgcgacgctg ccaccacatg aaaaagtccc acaactggaa
     2641 gtagccgaaa tcacccgctg tcgccatgcg cggatagtcg ctcatcatcg tgtgatagca
     2701 gaactcgtcc cgctcaaagt cggtgtaggg cgccgtcagc tccttcggtt gcgcatccca
     2761 cgcgcatcgc gtgatatagg gatgatccca ccagccgaac accacgcctg cagaaagcaa
     2821 taccgaaacg cagacaactc ttcgtgttgt catgtaagtc atccttgttt tcctatgaac
     2881 aattcttcca cctcacatgc ctccgctctc cgtttcccac tcgccgtacg tttccggttc
     2941 ctcgcggacg ttgctttcac ggtgggcttt caacaccagc atgtcccgtt gctccttgat
     3001 tttggccacc ccttcttcgc gctccttgcg cttggtctcg ttaagcgtcc gctgcaggtc
     3061 cgtgactcgc ggcgagtatt cgtaagaggt atacgtgctc aacttcaggt cgtagccgtc
     3121 caggcggacg aagttgttca tctggcggag ctgttcggcc acggcgggct tcagggcctc
     3181 cagttgcgct tcgatctcaa agagctgctt gcgcagctcg aaataacgtg tgatttcttt
     3241 tgagttcatt tttgccttct cctttttgga tggaagccga gttgttcacc gcgttttcca
     3301 ttacataaag tttagtttgg catacgtgcg agatgtcggc aaggcgagaa tgtggacacc
     3361 cttgcaatcg gttcgtgaca ggggg
//
LOCUS       DAOSLE010000218         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_24918_length_3337_cov_11.236746, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000218 DAOSLE010000000
VERSION     DAOSLE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24918_length_3337_cov_11.236746"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PK576_10360"
     CDS             <1..623
                     /locus_tag="PK576_10360"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62028.1"
                     /translation="KFTPKIHHPMQWRYNLEYSCGMLTDFAAHQLDIMQWALGKDGIE
                     PVAIENIKGRLPPSTDLYNTLAEPFSFEIVYGDGTRVFVSSEYTYGCRFYGENGKQIY
                     VTNGKIETNPPELVREKIGENEVRLIRSGQQEKNFIEAVYSGATPISPVQAGFSALVP
                     AVLAHIGMRTERASLKWLAAKTRFENAPDADKLLAPHMRAPWKLDV"
     gene            complement(654..1604)
                     /locus_tag="PK576_10365"
     CDS             complement(654..1604)
                     /locus_tag="PK576_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE62029.1"
                     /translation="MAKKTRTLYRRMRRPLEWCAVWVALAVIPSLSLRRLLGLSRWIA
                     DIGLLFDYRGKTIARANLALMFGPRMTPYRERVIIRHAYRNMARVLVNVFWLSRDTRT
                     RVLDQVAFVPSVLETLRAAHPAITVSAHFGNWEILSQACVAHGVPMMSVAKEIGSPEM
                     TAQLIRLRSTIGQEIVPAEGAIRPLIQALRKGKSIGLLIDQHTHLWEGGAWISFFGVP
                     AGISLTPAALALKLNRPVLFAWSRPLHDGRYRIEPGELFLPEPGLDAATLTQRIAAAF
                     ERVIRRHPSFWCLNYRRWRYIRSGDDPTRYPDYARPERIR"
     gene            1974..2591
                     /locus_tag="PK576_10370"
     CDS             1974..2591
                     /locus_tag="PK576_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOE62030.1"
                     /translation="MARKTKAEAEKTRQCILKAALRLFVEKGYDRTTFADVAQRIGLT
                     KGAVYWHFKSKPDLLAELVVLMTERHTTQLARALPHPVSLDTPEALTENFVLRARLIV
                     SSPASRRFFLMTRRLDWASPKLTPVRQRIRRLADTPHVVICSALKDMQRTGAIRADAD
                     LAAVSSVLMMIWLGLLEQEISNGLGTDLDGALRFGFQAILNGVTH"
     gene            2676..>3337
                     /locus_tag="PK576_10375"
     CDS             2676..>3337
                     /locus_tag="PK576_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE62031.1"
                     /translation="MGWLLTAACLLWLGWMARALAPQKAAEASMPSAAMMGGAGGAPP
                     VEVQAVTEEEVNLPNTYIGRVEPIQDVELRAQVGGYIKNIHFKEGADVKAGDLLFTID
                     PEQYEARVAVRKAEIGQAEALLDRAERYLNRLERSDARAITQADLDTARSDVAQGRAA
                     VKQAVANLALAEFDLKHTRIFAPIDGRIGRTVANVGDYVMPSLGALVRIVQTDPIRIA
                     FSV"
BASE COUNT          674 a          921 c         1115 g          627 t
ORIGIN      
        1 gaaaattcac gcccaaaatc caccatccga tgcagtggcg ctacaatctg gagtattcgt
       61 gcggcatgct gacggacttc gccgcgcatc agcttgatat catgcagtgg gcgctgggca
      121 aggatggcat cgaaccggtc gcgatcgaaa acatcaaggg gcgtttgccg ccgtccaccg
      181 acctctacaa cacgctggcg gaaccgttct catttgagat tgtttacgga gacggcaccc
      241 gcgtctttgt ctcgtcggaa tatacctacg gttgccgttt ctatggcgag aacggcaagc
      301 agatttacgt gaccaacggc aagatcgaaa ccaatccgcc cgagctggtc cgagagaaga
      361 tcggggaaaa cgaagtccgg ttgatccgta gcggacaaca ggagaagaac ttcatcgaag
      421 cggtgtacag cggcgcgacg ccgatttcgc cggtccaggc gggtttctcg gcgttggtgc
      481 ctgccgtgct ggcgcacatc ggcatgcgga ccgaaagggc ttcactcaaa tggctggccg
      541 cgaaaacgcg cttcgagaat gctcccgatg cggacaaact cctcgcgccg cacatgcgcg
      601 ccccgtggaa gttggatgtg tgacgggccg cgtgacgcgg ggccggggcg cgcttaacgg
      661 atgcgctccg gccgcgcgta gtccggatag cgcgtggggt cgtcgccgct ccggatgtac
      721 cgccaccggc ggtagttgag acaccagaag gaggggtgtc ggcggatcac ccgctcgaac
      781 gcggcggcga tccgctgggt gagggttgcg gcatcgaggc cgggttcggg caggaacaac
      841 tctcccggct cgatgcggta gcggccgtca tgcagcggac gggaccaggc gaagagcacc
      901 ggacggttca gcttgagcgc cagggcggcg ggcgtcaggg agatgcccgc cgggacgccg
      961 aaaaacgaga tccaggcacc gccctcccaa agatgggtgt gctggtcaat cagcaatccg
     1021 atcgatttgc ccttgcgcag cgcctggatc agcggacgga tcgcgccttc cgcgggtacg
     1081 atctcctggc cgatggtcga gcgcaggcgg atcagttggg cggtcatctc gggagagccg
     1141 atctctttgg cgaccgacat catcgggacg ccatgcgcca cgcaggcttg ggagaggatc
     1201 tcccagttgc cgaagtgggc actcaccgtg atggcggggt gcgccgcgcg tagagtctcc
     1261 agtacggacg gaacgaacgc gacctgatcc aacacacggg tgcgcgtgtc acgggagagc
     1321 cagaagacat tcaccaacac ccgggccatg ttgcggtaag cgtggcggat gatcacgcgt
     1381 tcgcggtatg gtgtcatgcg gggtccgaac atgagcgcca aattcgctcg ggcgatcgtc
     1441 ttgccgcggt aatcgaacag caaaccgatg tctgcaatcc agcgggagag cccgagcagc
     1501 cggcgcaacg aaaggcttgg aatcacggcc aacgccaccc atacggcaca ccactccagc
     1561 ggccgccgca tacgtcggta aagcgtacgt gttttctttg ccatagataa ttttagcata
     1621 gcaagccggg gcagtacggg aaaggcaaaa aaaacggtct tgccgtgttg taatgatttg
     1681 ttttcgcatg gagaaacttt tccgcgcacg atccggtttc ccggggatgc ggccttgccc
     1741 ggaggcggca cggtttcggc ggtccggctc cgtgctgatg gcgcatggct gcggacgtcc
     1801 cgccgcaccg gcagggaccg tctgcggctc gtggggacgc tcgctcttca gggcgtggcg
     1861 tcaagcaggg aaacagggat gcgcccgaat cggcgtcagg gtcggaatca gtcttgcata
     1921 cctacccgga ggtatgtata gtgtgcgcct atttcgcgaa gggaggggtg cgcatggcgc
     1981 ggaaaaccaa agcggaagca gaaaaaacgc ggcagtgtat tctcaaggcc gcgctcaggc
     2041 tgtttgtcga aaaaggttat gaccgcacaa cttttgcgga cgtggcgcag cgtatcggcc
     2101 tgacaaaagg cgcggtctat tggcatttta agagcaagcc tgacttgctc gccgagctcg
     2161 ttgtgctgat gacggagcgg catacgaccc agttggcgcg tgcgctgccg catccggtgt
     2221 cgctggatac accggaggcg ttgaccgaaa atttcgttct acgcgcccgc ctgattgtca
     2281 gttcgccagc cagccgacgt tttttcctca tgacgcgtcg gctggactgg gcatcgccta
     2341 aacttacccc agtgcgccag cggatacgtc ggctggcgga cacgccccat gttgtcatct
     2401 gttcggcgtt gaaagacatg cagcgcacgg gcgccatccg tgcggacgcg gatcttgcgg
     2461 cggtgtcgtc ggtgctgatg atgatttggc tggggttatt ggaacaggag atttcgaatg
     2521 gcttgggaac cgacctcgat ggtgcgcttc ggtttggttt ccaagcgatt ttgaatggcg
     2581 ttacgcattg attttgaaag aaaggtaccc gtatcatgag tgaaaatcta gaacaacaaa
     2641 aggttactaa acgtgaacac ccgatagcca aagttatggg ttggttactg acggctgcct
     2701 gtctgctgtg gttgggatgg atggcgcgtg ccttggcgcc acaaaaggcg gctgaggcat
     2761 ctatgccgtc ggcagccatg atgggcggag cggggggggc gccgccggtc gaggttcaag
     2821 cggttacgga ggaggaggtg aatctgccca acacctacat cggccgggtc gagccgattc
     2881 aggatgtcga gttgcgcgcg caagtcggcg gttatatcaa gaacatacac ttcaaagaag
     2941 gcgcggatgt caaggcgggg gacctgctct tcaccatcga ccccgagcag tatgaggcgc
     3001 gggtcgcggt gcgcaaggcg gagattgggc aagcggaggc gctgctcgat cgcgccgaac
     3061 gatacctgaa ccggctggag cgttcggatg cacgcgccat cacgcaggcg gatctcgata
     3121 ccgcgcgcag cgatgtcgcg cagggacgcg cggcggtcaa gcaggcggtc gccaacttgg
     3181 ctctcgcgga gttcgatctg aaacacacac gcatctttgc gccgattgac ggacgtatcg
     3241 ggcggactgt tgcgaatgtg ggtgactatg tgatgccgtc attgggagcg ctggtgcgga
     3301 tcgtacagac ggatcccatc cgcatcgcgt tttcggt
//
LOCUS       DAOSLE010000219         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_25110_length_3317_cov_8.383200, whole genome shotgun sequence.
ACCESSION   DAOSLE010000219 DAOSLE010000000
VERSION     DAOSLE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25110_length_3317_cov_8.383200"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..2631)
                     /locus_tag="PK576_10380"
     CDS             complement(316..2631)
                     /locus_tag="PK576_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HOE62032.1"
                     /translation="MKKTVLSACVCWAALTGVAGGNDLIIWDDAGAGDKWDLAYPVGN
                     GRLGAMPFGNFPKEKILINEETIWHRDPAKLMPEEGFTYLETVRRLEASGDYAGADRF
                     FEKQFQPDGTNPDSYQPFGWLEIEYVTVSPLRKRYRELDLTTGIAKNVYTLDNGSVIT
                     QNVFACGRDDVIAVTIAADSPIRLRVRIDGGTVRGREIIKAGAATGQDATRYVGHIRV
                     LSADSIRADEGFLDIAGVGPITLLVAVATDLDRRTAGAKLPEGWQQKAARTLDELDGV
                     SGDALQRRAVNEHRQYFGRVELDLGKTADAILALSTPERLKRIQAGSQDDPDLIETYF
                     QFGRYLLIASSRPGSFPANLQGVWNPHVKAPWGSDFHLNINLQMNYWLAETTNLPETH
                     QPLFDFIRYCLPSGREMARRMGMRGWCMGHATDIWANARVMSSRVYWGGSFFGGQWMV
                     LHLLDHYRFNRDKRVLENNWDILTDSAAFAESWLIPDPATGQLLSRPSSSPENAFRYA
                     AADGVMAKAALSSGTSFDQFMILQVFHDYLEAAEILGRTEEPLVKTIRAVLPKVYRPR
                     VAKDGRLMEWRLPFEEVEPGHRHISHVLGAYPGNQIDLDGDSVMRDAVRRTLEFRLKH
                     GGAHTGWSRAWTIGIFARLSDGARAYDNLHAILAKSTLPNLWDNHPPFQIDGNFGATA
                     AIAEMLLHSHNDQINLLPALPANWPTGKFSGLRARGDYTVSAVWRDGVLREAEIRAGR
                     NAAGPVRVTCQGRTRTISVKPGKTSTFTPGG"
     gene            complement(3070..3231)
                     /locus_tag="PK576_10385"
     CDS             complement(3070..3231)
                     /locus_tag="PK576_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62033.1"
                     /translation="MRRSYVSGFHYGAIAIGFGTAATFTLVIAGRFVGATVDSDSDTD
                     TDLEIWMEP"
BASE COUNT          668 a          999 c          986 g          664 t
ORIGIN      
        1 catccaaatc ggtatcgggg tcggtatcgc tatcggaatc gatcatttaa ccacgggaca
       61 cacgggatgg catcgctcgg gttttttggg aacgccaggg ggaattcctg attttcatga
      121 agtttcatgt gtttcacggt tccatccaaa tcggtatcgg ggtcggtatc gctatcggaa
      181 tcgactgtcg cgcccacgaa acggcccgca atgacgagtg taaaggtggc ggcggtcccg
      241 aacccgatcc cgatggcgac atctcaggaa gggaaggggc aagtgcctgc ttgcccggac
      301 gagcagacgc ccgtctcacc ctcccggcgt gaaggtgctc gtcttgcccg gcttcacgga
      361 gatcgtccgg gtccggcctt ggcaggtcac gcggaccggt ccggccgcat tgcgccccgc
      421 gcggatttcc gcctccctca ggacgccgtc ccgccacacc gcgctgacag tgtaatcgcc
      481 gcgcgcgcgc aatcctgaga acttccctgt cggccaattc gctggcaggg ccggcagcag
      541 gttgatctga tcgttgtggc tgtgcaggag catctcggcg atcgccgccg tggcgccgaa
      601 attgccgtcg atctgaaacg gcggatggtt gtcccagagg ttcggcaacg tcgatttcgc
      661 gagaatcgca tgcaggttgt cataggcgcg ggcaccgtcg gagagacgcg caaagatacc
      721 gatcgtccag gctctgctcc agccggtgtg cgcaccgcca tgcttcaaac ggaattccag
      781 cgtgcggcgg accgcgtcac gcatgaccga atccccgtcg agatcaatct ggttgccggg
      841 gtacgcgccc agcacatgcg agatatgccg gtgtccgggc tcgacctctt caaaaggcag
      901 ccgccactcc atcaagcgtc cgtccttcgc gacacgcgga cggtagacct tgggcagcac
      961 cgcccggatc gtcttgacga ggggttcttc cgtgcggccg agaatctccg ccgcctccag
     1021 ataatcatga aaaacctgta ggatcataaa ctggtcgaag gaggttccgg acgaaagtgc
     1081 ggccttggcc atcacgccgt cagccgcggc atagcggaac gcgttttcgg gtgaactcga
     1141 cggacgcgac agcaattgcc cggtcgcggg gtcgggaatc agccaggatt ccgcaaacgc
     1201 tgcggaatcg gtcaggatgt cccagttatt ttccagcacg cgcttgtccc ggttgaaccg
     1261 gtaatgatcg aggagatgca ggaccatcca ctgcccgccg aagaaagagc ccccccaata
     1321 cacgcgcgag gacatgaccc gcgcgttcgc ccaaatatcg gtggcatgtc ccatgcacca
     1381 tcctcgcatg cccatgcgac gcgccatctc gcgcccggac ggcaggcaat accggatgaa
     1441 atcaaacaga ggctggtgcg tttccggcag gttggtcgtt tccgcaagcc aataattcat
     1501 ctgtagattg atgttcagat ggaagtccga accccagggc gctttgacgt gcggattcca
     1561 aacaccctgc aggttggcgg ggaaagaacc gggacgcgag gaggcgatca acaggtaacg
     1621 gccgaactga aagtacgttt cgatcaggtc cgggtcatcc tgactaccgg cttggatccg
     1681 cttcagacgt tccggggtgg acaaggccag aatggcatca gcggtcttgc cgagatccag
     1741 ttccacgcgt ccaaagtatt gccggtgctc attcacggcc ctccgctgca acgcgtcccc
     1801 cgaaacgccg tccagttcat ccagagtccg ggccgctttt tgctgccagc cttcagggag
     1861 tttggcgccg gccgtgcggc ggtcaagatc ggtggcgact gccacaagaa gggtgatcgg
     1921 cccgacaccc gcgatgtcga gaaacccctc gtcggcacgg atgctgtcgg ccgacagcac
     1981 acggatgtgt ccgacgtacc gggtggcgtc ctgtccggtg gcggcacccg ctttgatgat
     2041 ctccctgccc cggaccgttc cgccgtcgat gcgcacacgg agtcggattg ggctgtccgc
     2101 agcgatcgtg accgcgatca cgtcatcccg tccgcaggca aaaacgtttt gggtgattac
     2161 cgaaccgttg tccagagtat agacgttttt cgcaatgccg gttgtcaggt caagttcacg
     2221 gtaccgtttg cggaggggcg atacggtaac gtactcgatc tccagccagc cgaacggctg
     2281 atagctgtcc gggttggtgc cgtctggctg aaactgtttt tcgaaaaacc ggtctgcgcc
     2341 cgcgtaatcg ccggatgcct caagccgccg cacggtttcc agatatgtga acccctcctc
     2401 gggcatgagt ttggcgggat cgcgatgcca aatcgtctcc tcgttgatca ggatcttctc
     2461 cttcggaaaa ttaccgaacg gcatggcgcc gaggcgcccg ttgccgaccg gatacgcgag
     2521 atcccattta tccccggctc cggcgtcatc ccagatgatc aagtcgttgc cgcccgccac
     2581 acccgtcagc gcagcccagc acacgcaggc ggaaagaacc gtctttttca ttgttacccc
     2641 ctatcccatg ctccgaaccg gattcggaac cgaaaacaca tctctcatca ggtttcagta
     2701 tgcgggggaa tggtgaaaag tcaagcgtcc acacggtcgg tgctgaatgt gggcccggac
     2761 agcgacggca acggcatccc cgacctgtgg gaaatgcgga agaccggcgg cctcgacgct
     2821 ctttgcgcca gagtgcgtgc acagcggagc ggttcacgtc cccgtacacg aaacacgaag
     2881 aggagtcatc gcgcacgggt acgcaaacgg gcgcgggtca ggttttcatc caaatcggta
     2941 tcggggtcgg gatcgctatc ggaatcgatt atttaaccac gggacacttt cttgggtttc
     3001 agggtttcac ggtttcacgg tttcagggtt tctggtttca aggtttcagg gtttctggtt
     3061 tcatgtgttt cacggttcca tccaaatctc caaatcggta tcggtatcgc tatcggaatc
     3121 gactgtcgcg cccacgaagc ggcccgcaat gacgagtgta aaggtggcgg cggtcccgaa
     3181 cccgatagcg atggcaccgt aatggaaccc agaaacgtat gatcttcgca catccctgtc
     3241 acgaaccgat tacgaatcgg tgttgacgcg caatcccaat ttctgcgaca ctaaagataa
     3301 ggtgatgtcc tcactgt
//
LOCUS       DAOSLE010000220         3287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_25406_length_3287_cov_7.180693, whole genome shotgun sequence.
ACCESSION   DAOSLE010000220 DAOSLE010000000
VERSION     DAOSLE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25406_length_3287_cov_7.180693"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..803)
                     /locus_tag="PK576_10390"
     CDS             complement(12..803)
                     /locus_tag="PK576_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOE62034.1"
                     /translation="MRAVILHLLCFALAALLHLLLLLLVGWHLVERGKEVWPELEVAS
                     VELTLEGPAPETPAVMSRRTVPEATPPPEPAALPEPPQDLPPLPEATPLSPPESLPTP
                     PEPRPLPEPARAPVRELPEPEARPVPEPVRVPVREQPEPEARPVQAEPGPQVEEAEAE
                     IREQGGAAVGQIIGHPLLEQTIRPTYPVGARRRGEEGTVILDVTVGPDGRAVTVTQVA
                     SSGFPDLDRAAERAAAQARFKPGTRDGKPVTSAARLTLIFRLRDR"
     gene            complement(820..1221)
                     /locus_tag="PK576_10395"
     CDS             complement(820..1221)
                     /locus_tag="PK576_10395"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE62035.1"
                     /translation="MRLNSGYEDRKARLEMISLMDVMFLILVFFVYSIFSMAVHRGLK
                     VELPAARGVPEQGERVIVTLAADGTLSLNRRPLALEDLVRGVTLLWRETGTPVLISTD
                     RAAPLGVGIELLGRLKEGGVERVTFQVSGKP"
     gene            complement(1225..1959)
                     /locus_tag="PK576_10400"
     CDS             complement(1225..1959)
                     /locus_tag="PK576_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE62036.1"
                     /translation="MMFGWGVQIMAQITEPQGRLAAAFTLFDRGGPVMWPILAVSLMG
                     LTVTFERLFAFWRYNTANFCFRRRQSKLIALTREGRFEEAVACARQTDSPVCRVLVKA
                     LENRDSGFQETLEAASQLELDRLRRGLSVLDTTITVAPMLGILGTVTGIINTFNMLNL
                     EGIENPAAATAGIAEALITTAAGLIVAIACLFPFNFLVSQLKRRTHELEQIIHQFALA
                     FNTGVRVAEKPGTGAGTGRCRNVTEE"
     gene            complement(2194..3174)
                     /locus_tag="PK576_10405"
     CDS             complement(2194..3174)
                     /locus_tag="PK576_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOE62037.1"
                     /translation="MDNATILSRAREVMETEIEGLRAAQAALGDDFLATVRLLTETLD
                     RDGKIVVTGVGKNLHIAEKLSATLASTGSTSVVMNPIQAMHGDLGMIAARDVLLALSF
                     SGESEEVIRLIPAVRRLEVPVIAMVGNPESTLARLSDVTLCVRIGREACPFGMAPTTS
                     TTATLAVGDALAMVLIDARQFDKASYARLHPAGAIGRALVLRVRDIMRTGERLASLRS
                     DASIMDAVMAMTAAQSGAALMTDPEGRLVGIFTDGDLRRLLSEGRAGLHEPLAPVMTR
                     NPKSVADDAFAVDVLRVFERYKIDDLPVVDARGLLVGVVDIQDLPKMKVM"
BASE COUNT          641 a         1096 c          976 g          574 t
ORIGIN      
        1 tctttttagg tttaccggtc gcgcaggcgg aagatcagcg tgagacgggc tgcggaagtg
       61 accggtttac cgtcgcgtgt gccgggtttg aagcgtgcct gagccgcggc acgttccgcc
      121 gcgcggtcca gatcggggaa accgctggaa gccacctgtg tcaccgttac cgcacggccg
      181 tccggcccca ccgtcacatc gagaatcacc gtgccctcct cgccgcgtcg gcgcgcgcca
      241 accggatagg tcggccggat cgtctgttcg agtaagggat gcccgatgat ctggccgacc
      301 gcggctccgc cttgttcccg gatttcagcc tccgcctcct ccacctgcgg acccggttcc
      361 gcttgcacgg gccgcgcttc cggctcgggc tgttcccgaa ccgggacgcg gacaggctcc
      421 ggcacgggcc gcgcttccgg ctcgggcagt tcccgaaccg gggcgcgggc aggttccggc
      481 aggggacgcg gttccggcgg ggtcggcaga gattccggcg gagacaaggg cgtcgcctcc
      541 ggaagcggcg gtaaatcctg cggaggttcc ggtaatgcgg caggctcggg aggcggcgta
      601 gcctccggca ccgtacgccg tgacatgact gcgggtgttt ccggcgcagg accctccaac
      661 gtcagttcca ccgaggcaac ttctaactcg ggccacacct ccttcccgcg ctcgaccaaa
      721 tgccacccga ccaacagcag cagcagcaga tgcaacagcg cggcaagcgc gaaacagaga
      781 agatgaagga tcaccgcccg catgggatga ggcctcccct cacggtttcc cactcacctg
      841 aaaggtgacg cgctcgaccc cgccctcttt gagccggccc aagagttcga tcccgacacc
      901 aaggggcgcc gcccgatccg tgctgatcag caccggcgtg ccggtctcgc gccagagcaa
      961 ggtcacgccg cgcacgaggt cctccaaagc caacggccgt cggttcaatg acaacgtgcc
     1021 gtccgccgcc agagtgacaa tgacccgctc gccctgctcc ggcaccccgc gcgccgcggg
     1081 caactccact ttcaggccgc ggtggaccgc catggagaag atcgaataga cgaagaagac
     1141 gaggatcagg aacatgacat ccatgaggga gatcatttcc agccgggcct tgcggtcctc
     1201 atatcctgaa ttcaggcgca taggtcactc ttctgtcaca ttgcggcatc gtcctgtccc
     1261 ggcccccgtc cccggttttt ccgcaacgcg gacaccggtg ttaaaggcca gcgcgaactg
     1321 atggatgatc tgctccaact cgtgggtgcg gcgtttgagt tgggacacca gaaaattgaa
     1381 cgggaacagg caggcgatgg ccacgatcag tcccgccgcc gtggtgatca gggcctcggc
     1441 aatgcctgcg gtagcggccg ccggattctc gataccctca aggttgagca tgttgaacgt
     1501 gttgatgata cccgtgaccg taccgagaat gccgagcatc ggcgcgaccg tgatggtggt
     1561 gtccaacacc gagagccccc ggcgcaggcg gtccaactcc aactgcgagg cggcttcgag
     1621 tgtctcctga aaccccgaat cgcgattttc caatgccttg acaagtacgc ggcacaccgg
     1681 cgaatcggtc tgacgcgcac aggcgaccgc ctcttcgaac cgaccctcgc gggtcagggc
     1741 gatcaacttg ctctgacgtc tgcggaaaca aaaattcgcg gtgttgtagc gccagaaggc
     1801 gaagagacgc tcgaacgtca ccgtgagccc catcagcgaa accgccagga ttggccacat
     1861 caccggaccg ccgcggtcaa acagcgtgaa ggccgctgcc aaacgcccct gcggctccgt
     1921 aatctgcgcc ataatctgta cgccccatcc aaacatcatg caacgttcct taccgttagg
     1981 gacggttccc cgaaccgtcc tgacgccttg gggaagcgtc cctacccaaa gcagccacac
     2041 gttcaaacca tacgggacaa cccgcagccc aattctttag ccgggattac aggacttaca
     2101 gggttttttg attctcggta atcatgtgag tcctgtaatc ccggctgtga attctcgctt
     2161 cgtctcttca aacgcttcac ccttgcccac ggttcacatc accttcattt tgggcagatc
     2221 ctgaatatcg acaaccccga cgaggagccc gcgcgcatcc accaccggca gatcgtcgat
     2281 cttgtagcgt tcgaacacgc gcagcacatc caccgcaaag gcgtcgtcgg cgaccgattt
     2341 ggggttgcgg gtcatcaccg gtgccagagg ctcatgcaga cccgcccggc cttcggaaag
     2401 cagccgacgc aaatcgccgt ccgtaaagat cccgacaagc cgtccttccg gatcggtcat
     2461 cagggccgcg ccgctctggg ccgcagtcat ggccatcacc gcatccatga tcgaggcatc
     2521 ggaccgcaag gaggcgagac gttcacccgt acgcatgatg tcgcgcacgc gcaggaccag
     2581 tgcgcggccg atcgcacccg ccggatggag ccgggcgtaa ctggctttgt cgaactggcg
     2641 tgcatcgatc agcaccatag ccagcgcgtc gcctacggcg agggttgcgg tagtgctggt
     2701 ggtgggcgcc ataccaaagg ggcaggcctc ccgtccgata cgcacacaca gcgtcacatc
     2761 gcttaaccgc gcgagggtgc tctccggatt gccgaccatg gcgatgaccg gcacctcgag
     2821 acgccgcaca gcggggatca gccggatgac ctcttccgac tcgcccgaaa aactcagcgc
     2881 cagcagcaca tcccgggccg cgatcatccc gaggtcaccg tgcatggcct gaatggggtt
     2941 catcaccaca ctggtcgaac cggtgctggc cagcgtggcc gagagtttct cggcgatgtg
     3001 gagattttta cccacacccg tgaccacaat cttaccgtcg cggtcgagcg tttccgtcag
     3061 cagacgaacg gtcgccagaa aatcatcccc cagagcggcc tgcgccgcgc gcagcccctc
     3121 gatctcggtt tccatcacct cccgggcgcg ggagagaatc gttgcgttat ccataatgcg
     3181 ttttattgta ccgcaacggt ttcgggaagc aagggaggaa tcaaggattt ccaaggtcat
     3241 gcgtttcagg gttccattgc ggcgccatcg ctatcgccat cgggatc
//
LOCUS       DAOSLE010000221         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_26619_length_3171_cov_5.671053, whole genome shotgun sequence.
ACCESSION   DAOSLE010000221 DAOSLE010000000
VERSION     DAOSLE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26619_length_3171_cov_5.671053"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PK576_10410"
     CDS             complement(<1..980)
                     /locus_tag="PK576_10410"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOE62038.1"
                     /translation="MSVISGIRLEKKVWSGLAFCVRGWCAGLAMGLLGIQAAAAGTVV
                     SRVTPGGGMPSLTWESYPLGVYRVWRSENAAGPYDTQVGGDVTATGYTTTLTDPQSGA
                     AGSRRYYRIETVSPGYANYLILDVSGGTNAASWPVVVTNAVPDLLTDPAYRTTKVVLK
                     LIGAGSFKMFGLYDVTLTRPFYIGVFELTQDQCVNMTGSAYASQDHGATRPANNFSFA
                     DLRGGTNDAAGWGWPADNLVAPNSLIGRLRAKTGSDNFDLPTSAQWEYASRAGTTTDF
                     YNGTDANPSDGTDFSMVTNIARCKANNDIPIDGQTGHAPVGSYLPNAWGI"
     gene            complement(994..2448)
                     /locus_tag="PK576_10415"
     CDS             complement(994..2448)
                     /locus_tag="PK576_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62039.1"
                     /translation="MGVTNQARGWVRARGLCRAMAGCGVLVCATGFSVRAGVVPNAGA
                     EAAVACVSAAPADGLFSAVFATNVAEVTFGAVGTDRALSLSCVSIDGTVASGASPTGM
                     AAFSQGTFDAARTGVSVRLGRVSGELHGTGPDQAFGALAWSGAGGLTNPATLWAGLID
                     GVVSANAGPDGTLAAALCAGTAVDVTLGSGAVLFGGTYGWDTGDGSAASAASALQTLL
                     AKVKARTATAAESNALVTAAADGFAVLGGHGEDTLAFASGNTAVFGTVALGEGYDRVA
                     LAGVASGERVSLPTMDGVELLAVTNCPDVSVANTAGLADLFVGSDSLCRLKDASCVFD
                     GLYGTGTVVPEGTLQATGMIAVARFEPAVTGAVLSVNGNLTLGNGVTCALRTAEEEGA
                     WVNDRVAVSGALTVAGGGTIDLGCAEEAPLALNTRLVLFTAADGIANPGALSSWQLIG
                     TGRDDERKVIRTIRAEGNAVVAVIAVGGTFISLR"
BASE COUNT          602 a         1156 c          872 g          541 t
ORIGIN      
        1 taaatccccc aggcgttggg caggtaggag ccgaccggcg catgccccgt ctgcccgtcg
       61 atcgggatgt cgttgttcgc cttgcagcgc gcgatgttgg tcaccataga gaagtccgtg
      121 ccatccgaag gattcgcgtc cgtgccgtta tagaaatccg tcgtcgttcc ggcacggctg
      181 gcgtactccc actgcgcgct ggtcggcagg tcgaagttgt cgctgccggt tttggcccgc
      241 agacggccga tcagcgaatt cggggcaacc agattgtcgg cgggccagcc ccatcccgcc
      301 gcatcgttcg tgccgccgcg caaatcggcg aatgagaaat tgttcgccgg acgggtggcg
      361 ccgtgatcct ggctcgcata agccgaaccg gtcatgttga cgcactgatc ttgagtcagc
      421 tcaaacacgc cgatatagaa gggccgggtc agggtcacat cgtataagcc gaacatcttg
      481 aaactgcccg cgccgatcag tttcagcacc accttcgttg tccggtacgc gggatcggtc
      541 agcaggtccg gcacggcatt ggtcacaacc accggccatg atgccgcatt cgtgccgccg
      601 gacacatcga ggatcaggta gttcgcatac ccgggcgaca ccgtctcgat ccggtagtac
      661 cggcggctgc ccgcggcccc ggattgcgga tccgtcagtg tcgtcgtgta ccccgttgcc
      721 gtcacgtcgc ccccgacctg cgtgtcgtac ggtccggccg cattttccga ccgccagacg
      781 cggtacacgc ccagcgggta cgactcccag gtcagggagg gcatgccacc gcccggcgtg
      841 acgcgtgaaa cgactgtccc cgccgcagcc gcctgaattc cgagcaaccc catcgccaac
      901 cccgcgcacc agccccgaac gcaaaaggcc agcccgctcc acactttctt ttccaaccgg
      961 attcctgaaa tcacgctcat gtctgcctcc tcctcaacgc aacgaaatga atgtgccgcc
     1021 caccgcgatg actgccacga ccgcgttacc ctccgcacgg atggtccgga tcaccttccg
     1081 ctcgtcatcg cgtccagtgc caatcagttg ccagctcgac agcgcaccgg gattcgcgat
     1141 cccgtccgca gccgtgaaca ggaccaggcg cgtgttcaac gcgaggggtg cctcctcagc
     1201 gcatcccaga tcgattgtgc cgccgcccgc cacagtcaac gccccgctga cggccacccg
     1261 gtcgttgacc catgcgccct cctcctctgc cgtgcgcaag gcgcaggtca ccccgttgcc
     1321 cagggtcaga ttcccattga cgctcagcac cgcgccggtc accgcaggtt cgaagcgcgc
     1381 gaccgcgatc atcccggtcg cctgcagcgt cccttccggc acgaccgtgc ccgtcccgta
     1441 aagcccgtca aacacgcagg aagcatcctt cagccgacac agggagtcgc tccccacaaa
     1501 cagatccgca agccccgccg tattcgcgac cgacacatcc gggcagtttg tgaccgccag
     1561 cagttccacc ccgtccatcg ttggcaggct caccctctct cccgacgcca cacccgccaa
     1621 ggccacccgg tcatacccct cgcccagggc gaccgtaccg aaaaccgccg tgtttccgct
     1681 cgcgaacgcc agcgtgtcct ccccgtgtcc gccgagcacg gcgaacccgt ctgcagccgc
     1741 cgtaaccaag gcgttgcttt cggccgcagt ggccgtccgc gccttaacct tcgccagcag
     1801 ggtctgcagg gccgacgccg cgctggcggc cgaaccgtcc cccgtgtccc atccgtaggt
     1861 tccgccaaag agcaccgcac cggagcccag cgtcacgtcc accgccgtgc cggcgcagag
     1921 cgccgccgcc agcgttccgt ccgggccggc attcgcgctc accaccccgt cgatcaaccc
     1981 cgcccacagg gtggccgggt tcgtcaatcc acccgctccc gaccacgcca gcgcaccgaa
     2041 cgcctgatcc gggccggtgc cgtggagttc gccgctcacg cgtccgagac gcaccgacac
     2101 cccggttctg gccgcatcaa aggtcccctg cgaaaaagca gccatgccgg tcggtgaggc
     2161 cccgcttgcc accgtcccgt cgatcgagac gcacgacagc gacaacgccc gatccgtccc
     2221 gaccgcgccg aacgtcacct cagccacatt cgtcgcgaag accgctgaga acagcccgtc
     2281 ggcaggtgcc gcgctcacgc aggccactgc ggcctccgca cccgcattcg gaacgactcc
     2341 cgcccggacg gaaaaacccg tggcgcacac caagacgcca caccccgcca tcgcacggca
     2401 cagcccgcgc gcgcgcaccc atccccttgc ctgattggtc acacccatac cactcctcct
     2461 tccgcctgtc ggccaaacct tgtctcgttg atttatatta taggaaaaaa aggcgtgttc
     2521 cgacaatctc gataatgcga actcgaccct cgattttccg aactaacgaa accccctcag
     2581 aacacctcgg gacggaaaca gggatgtgcg aaacccatgc gtttctggtg aaagggagag
     2641 cagagagtcg gaagcgcggg cacctctggt ggacattgtt gactttgttg acgttgtgtg
     2701 gagacctgga cggcggaagc ggtgagggta attttgtctc acactgcaaa acagcgccat
     2761 tgtggctcac ttggttctgc ggatatgtgg agccatgaaa cacctccact ttttgtgacc
     2821 actcagtggt cacaaagatg aggttaaaac cttgcaccat gcagccgcgc cccgcccgcg
     2881 gtcatttaca gcacttcggg cgcttttggg ggctgtctcc gcgcgcgccc tatcgcatgc
     2941 cccgcgcgcg cccggcgccc gccctcccac ctgtttttac actcatcatt acgaccactt
     3001 ggtggtcgca ataaaaatct gtggatgaaa aaaaaccgag ccatgccttt tcgtgttgtg
     3061 gcacggtggg tgcttatatc agggtacgac aaggagacca caccatgacc gatttgaaca
     3121 aatggaccca aaaagcgcag gaggcgctgc agaacgcgcg ccaactcgcc a
//
LOCUS       DAOSLE010000222         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_26811_length_3153_cov_7.604261, whole genome shotgun sequence.
ACCESSION   DAOSLE010000222 DAOSLE010000000
VERSION     DAOSLE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26811_length_3153_cov_7.604261"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1678
                     /gene="hemL"
                     /locus_tag="PK576_10420"
     CDS             401..1678
                     /gene="hemL"
                     /locus_tag="PK576_10420"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOE62040.1"
                     /translation="MRRYKVDHEELYTRACRVIPGGVNSPVRAFNAVGGVPLYVESGK
                     GDKLRTADGRTLTDFCCSWGAMILGHAHDEVVEAIARQAARGTTFGINTPEEVTFAER
                     LTALVPGLERVRLVNSGTEAVMTALRIARGVTGRKRIIKFDGCYHGHSDSVLVGAGSG
                     LLTGGTLHSLGISRHVANDTFVAPFNDIDAVNEIISEKRVEIAAVIVEPVPGNMGLVM
                     PDPDFLVKLRRAADRCGALLIFDEVITGFRFGLSTYAQRVKVKPDLITLGKVIGGGLP
                     LAAVGGAAVFMDGLAPDGKIYQAGTLSGNPLALAAGLKTLEVLERDDPYPRMAQIGCR
                     MAEQINTAAAAKGLPLRCAQFQGVFTLFCNAEPVRNLTDAKRSDTQQYARLFHKLLQQ
                     GYYLPPSQFEVGFISAAHSEEAVMAFAQAVTDE"
     gene            1724..2560
                     /gene="panC"
                     /locus_tag="PK576_10425"
     CDS             1724..2560
                     /gene="panC"
                     /locus_tag="PK576_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOE62041.1"
                     /translation="MQVFTQPETMRQWAEEQRRAGRRIGLVPTMGALHAGHLSLVAEA
                     RRRADAVAVTIFVNPAQFGPNEDYAAYPRTETHDLACCREAGVDAVFLPSTTAMYAPD
                     ASVFVEEESLSLGLCGARRPGHFRGVCTVVAKLFHIALPHVAVFGQKDFQQAAVIRRM
                     VRDLDFPVEVVVAPIIREPDGLAMSSRNAYLTEEERQRAPGLSCALRQAAEAVAAGPC
                     DAETVRRNMTDVLVEHALKPDYVEIVDPDTLEPVAEARQGMVALVAAYCGKTRLIDNQ
                     VL"
     gene            2752..>3153
                     /locus_tag="PK576_10430"
     CDS             2752..>3153
                     /locus_tag="PK576_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HOE62042.1"
                     /translation="MDEAVLTDKEQRLHVLLRQYGRVAVGFSGGVDSTLLLRVARDVL
                     GPEAVMAVIADTPSMPRSEFAEACALADAMGVVRVVTDSTELADPRYAENSSDRCYYC
                     KKHLFARVAALAAEKGFHAVLDGNNADDAGDY"
BASE COUNT          588 a          823 c         1135 g          607 t
ORIGIN      
        1 tgttcatcgg gccttgtgtg gtcttgaccg acaactccgg aaacttatcg cccaacaacg
       61 gcatgctgtt ctttgacatt tccatgttat gttacctccc aactgagtga taaaacgtgt
      121 ctgccagatc acaccgcatg gcgtgaaccc gacccgaagg aacttccttc aactgtcaga
      181 ttattctgaa aaaagactcg ggtgtcaacc cccggcaatc ggttggcggg gggcgggacc
      241 gaaatcggta tcgggaggcg aaatgcaata tcaacttcca gtcccgatgc cgccgtccct
      301 tttcttattg caagtcactc cttcatcgcg ccctctaggt tgaccgttcg atgcgatatt
      361 ggtaatgtga ttatgtgcct tttaaaaaac gaaaacaggc gtgaggcgct acaaagtgga
      421 tcacgaagag ttgtatacgc gggcgtgccg ggtgattccg ggcggggtca acagtccggt
      481 tcgggctttt aacgctgtgg gaggggtgcc gctctatgtc gagtccggca agggcgacaa
      541 gctgcggacg gcggacggac ggacgttgac ggatttctgt tgttcgtggg gcgcgatgat
      601 ccttggtcac gcgcacgacg aggtggtgga ggcgatcgcc cggcaggccg cgcggggcac
      661 gacgttcggc atcaacaccc cggaagaggt gacgtttgcc gagcgcctca cggctctggt
      721 gccggggctg gagcgggtgc gcctcgtgaa ctccggtacc gaggcggtca tgaccgcgtt
      781 gcgtatcgcg cgcggggtga cgggccgcaa gcggatcatt aagttcgacg gctgttatca
      841 cgggcacagt gacagtgtgc tggtcggtgc gggcagcgga ttgcttaccg gcggaacgct
      901 ccactcgctc gggatctcgc ggcatgtggc taacgataca ttcgtcgccc cgttcaacga
      961 catcgacgcg gtcaatgaaa tcatcagcga aaagagggta gagatcgccg ccgtcattgt
     1021 ggagccggtg ccgggtaaca tggggctcgt gatgccggat ccggactttc tggttaaatt
     1081 gaggcgggcg gcggatcggt gcggcgcgct gctcattttc gatgaggtca tcactggatt
     1141 ccgcttcggc ctctccacat acgcgcaacg cgtcaaggtg aaacccgacc ttatcacgct
     1201 gggcaaggtg atcggcggtg gcttgccgct ggcggcggtg ggcggggccg cggttttcat
     1261 ggacgggttg gcgccggacg ggaaaatcta tcaggcggga acgctctccg gaaatccgct
     1321 ggcgctggcc gcgggtctga aaacgctgga ggtgctcgaa cgggatgatc cctacccgcg
     1381 gatggcgcag atcgggtgtc gcatggcgga gcagatcaac acggcggcgg ccgccaaagg
     1441 gttgcccttg cgatgcgcgc agtttcaggg cgtgttcacg ctcttctgca atgctgagcc
     1501 ggtgcggaac ctgacggatg ccaagcggag cgatacgcaa cagtatgcgc gacttttcca
     1561 caagctgctg caacaggggt attatctgcc gccctcgcaa ttcgaggtcg ggttcatctc
     1621 ggccgcgcac agcgaggagg cggtcatggc gttcgcgcag gcggtgaccg acgagtgacg
     1681 gtggcggtcc gcggctgttg gctatgcggg gaagggaaag ttgatgcagg tgttcacaca
     1741 gccggaaacg atgcggcagt gggcggagga acagcgtcgt gccggcaggc ggatcgggct
     1801 tgtgccgacc atgggggcgt tgcacgcggg acatctgtcg ctggtcgcgg aagcgcggcg
     1861 gcgggcggat gccgtggcgg tcacgatttt tgtgaatccg gcccagttcg ggccgaatga
     1921 ggactacgcg gcctatccgc gcaccgagac gcacgatctc gcgtgttgcc gggaggcggg
     1981 ggtcgatgcg gtgtttctgc cgtcgactac ggcgatgtat gcgccggacg ccagtgtgtt
     2041 cgtggaggag gagagcctct ccctcggatt gtgcggtgcg cggcgtcccg gacatttccg
     2101 gggagtgtgt accgtggtgg cgaaactgtt tcacatcgcg ctaccgcatg tggcggtgtt
     2161 cggtcagaag gactttcagc aggccgccgt gatccggcgg atggtgcgcg atctggattt
     2221 tccggtcgag gtggtggtcg cgccgatcat ccgcgagccg gacggtttgg cgatgagttc
     2281 gcggaatgcg tacctgacgg aagaggaacg acagcgtgcg cccggattga gttgcgcgct
     2341 gcggcaggcg gcggaagcgg ttgccgcagg gccctgtgat gccgaaacgg tgcggcggaa
     2401 catgacggac gtgttggtgg aacacgcgct gaagccggat tacgtcgaga tcgtagaccc
     2461 cgatacgctg gagccggtgg cggaagcgcg gcagggaatg gtggcactgg tcgcggccta
     2521 ttgcggaaaa acgcggttga tcgacaatca ggtgctgtag acgcaaatac gggcggagtc
     2581 caaagccaag gaggcggggg gagagggaaa cggggaatcg gaacgcccaa tgtcgaacgt
     2641 tgaacacgga acgccgaagc atcggtaggg aggcatcccc gaggcgtccg cagatgggac
     2701 aatcgggatc gcacgcttga aacggcgaac cggagaacgg gaacgctggg tatggacgag
     2761 gcagtgctga cagacaaaga acaacggctt catgtgttgc tgcggcaata tggccgggtg
     2821 gcggtcggat tttcgggtgg tgtggacagc acactcttgt tgcgcgtcgc gcgggatgtg
     2881 ctggggccgg aagcggtgat ggcggtgatt gccgacacgc cgagtatgcc gcgcagcgag
     2941 ttcgccgagg cgtgcgcgct ggccgacgcg atgggggtgg tgcgcgtggt gacggattcg
     3001 acggagttgg ccgatccccg gtatgccgaa aactcttcag accgctgtta ttattgtaag
     3061 aagcaccttt ttgcgcgggt tgccgcgctc gccgcagaga agggttttca cgcggttctc
     3121 gatgggaaca acgcggatga cgcgggcgat tac
//
LOCUS       DAOSLE010000223         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_27258_length_3109_cov_11.882777, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000223 DAOSLE010000000
VERSION     DAOSLE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27258_length_3109_cov_11.882777"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PK576_10435"
     CDS             <1..426
                     /locus_tag="PK576_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62043.1"
                     /translation="EYEEAKKGYESYAKRDEKEPAVYSRKRWYKRTLDRYEAQQKSDP
                     VNTLEIHVLRVGDLALATNPFELFADYAMRMHGRSPAGQTMLVQLASPADVTHSYLPS
                     ERAVKGGGYSAVPQSTPVGPEGGQILVERTLDTIRALFP"
     gene            636..980
                     /locus_tag="PK576_10440"
     CDS             636..980
                     /locus_tag="PK576_10440"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOE62044.1"
                     /translation="MAGTTYEMTGSIKTILEPMTFASGFTKREFILTMEDDYPQDVKF
                     ACVKDRTALLEHVAVGERVKVGFRIRSREYKERYYTDLEAFRIDKLEMDGSSVSYEDA
                     EPLTPFDDPTPF"
     gene            1069..1938
                     /gene="prmC"
                     /locus_tag="PK576_10445"
     CDS             1069..1938
                     /gene="prmC"
                     /locus_tag="PK576_10445"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOE62045.1"
                     /translation="MAEEQKKLRELIALGDAYLAKRNVPDSRVACELLAARLFRCARL
                     DLYRHLDTIPETRLVDALRRGLQRVAGGEPVQYVLGQWAFRNLTLKVDRRALIPRPET
                     EQLVGLVLRSPQVRDTVKPLVVDVGTGTGCIILSLAQELGRGVFVGLDICADALALAQ
                     ENAELTGLAGRVLFAQSDGCGEFDPASVDVLVSNPPYIPSHIVDGLEPHIRDHEPRLA
                     LDGGPDGLTFFRALLLDAVMVLKPGGAVFFEIGDEQGPALRALLDEYGFTEVAIMQDD
                     SGRDRYAMGVQSV"
     gene            complement(2820..>3109)
                     /locus_tag="PK576_10450"
     CDS             complement(2820..>3109)
                     /locus_tag="PK576_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62046.1"
                     /translation="VECRGSRVDDPDPDSDPDPDSDPDPDLDETMKYMKLHENQEFPL
                     AFQKNRAMPKKICVICVNLWIKTRAVPSRVFVVKQSIPIAIAIPTPIWMKP"
BASE COUNT          640 a          739 c         1005 g          725 t
ORIGIN      
        1 gagtacgaag aggcaaaaaa gggatatgag tcctacgcca agcgtgacga gaaagagcca
       61 gcggtgtata gccgcaagcg ttggtacaag cggacgttgg atcggtatga ggcgcagcag
      121 aagagcgatc cggtcaacac acttgaaatc cacgttctgc gcgtgggtga tctggcgctg
      181 gcgaccaatc cgttcgaact gttcgcggat tatgcgatgc gcatgcacgg gcgcagtcct
      241 gcgggccaaa cgatgctggt ccaactggcc agtccggcgg atgtgactca ctcctatctg
      301 ccttccgagc gggcggtcaa aggcggagga tacagcgctg tgccgcagtc cacgccggtc
      361 ggccccgaag gcgggcagat tctggtggaa cggactctgg atacgatccg tgcgctgttt
      421 ccctgaaacc ggagccccga aatgatggcg tgagccgctt ccggtgttgc ccgcaacgct
      481 ccggtttggt ttaatggccg caccgtaaga aaacggcagg ccaaccatcc ggggtgggcg
      541 aaaacgagag ggcaacggga acgttcaatg ttaagaaaag tcaggaatca acaaggtttc
      601 tgattgaagg tatagacaag cgaggacttt gggatatggc tggaacgacc tatgagatga
      661 cggggagcat caagacgatt ttggagccga tgacctttgc gagtggtttc acgaaacgcg
      721 aattcattct caccatggaa gatgactatc cgcaggatgt caaattcgcc tgtgtgaaag
      781 atcggacggc cctgttggaa catgtggcgg tgggagagcg tgtcaaggtg gggttccgga
      841 ttcggagtcg ggagtacaag gagagatact atacggatct ggaggccttc cgcatcgaca
      901 aattggagat ggacgggtct tccgtgtcct atgaggacgc ggagccgctg acgccgttcg
      961 atgacccgac gccgttctga ttgccggtcg gtgatacgga ccttcctttt caagacgggc
     1021 aaggcctgga ggattgattc ctgccccgac catgcggatg cggaacgaat ggccgaagaa
     1081 caaaaaaaac tccgtgagtt aattgcgttg ggtgacgcct atctcgctaa gcggaacgtt
     1141 cctgattccc gcgtggcctg cgaactgttg gcggcacggc ttttccggtg tgcccgtctg
     1201 gatttgtacc gccatctcga cacgattccg gaaacgcgtc tagtggatgc gttacggcgc
     1261 ggtctgcaac gtgtggctgg aggggaaccg gtacagtatg tgctgggcca atgggccttc
     1321 cgtaacctga cgctcaaagt ggatcggcgg gcactgatcc cgcggcccga aacggagcaa
     1381 ctggtcggtc tggttttacg ttccccgcag gtgcgggaca cggtgaagcc gttggtggtg
     1441 gatgtcggca ccggcacggg gtgcatcatt ctgagtttgg cgcaggagct ggggcgtggg
     1501 gtttttgtgg ggctggacat ctgcgcggat gcgctggcct tggcgcagga gaatgcagag
     1561 ttgaccgggt tggccggacg ggtgttgttc gcgcagagtg acggttgcgg cgaattcgat
     1621 cccgcaagcg tggatgtgct ggtctcgaat cccccctaca ttccttcgca catcgtggac
     1681 ggtctcgaac cgcacatccg cgatcatgag cctcgtctgg cgcttgatgg cgggccggat
     1741 gggctgacat ttttccgcgc gctgctgctt gatgcagtga tggtgctgaa acccgggggc
     1801 gcggtttttt ttgaaatcgg ggatgagcag ggccctgcgt tacgtgcgtt gctcgacgag
     1861 tatggtttta cggaagtggc cattatgcaa gatgattcgg gtcgggatcg gtatgccatg
     1921 ggcgtgcaga gcgtgtgagc ggtatcgtgt ccggggctga ccggttgggt ttgccttgga
     1981 gcggcagaga acaagagtgc ccggaacgct gaacgtgacc tgtgacgttc agatttcggg
     2041 cttcaagttt catccgcaga ttacgtagat ggacgcaggt ttttgggggg cgagagtctc
     2101 cacgagttac ctagtccaaa cacttcgcgg atcacgacga aagccgatgt ttgcgaggct
     2161 ttgttcgaat tctgcccgtt caactgccgg ttatagggta accggagtaa gggaaaaaga
     2221 ggtggggtct gtccacatct catgataagt gggcagaccg gcgccctgcg gaacggaaga
     2281 aacgaacggg ccgggcaaac cgtgaagggc ggccgttgaa gtgagtgttg cttatccgtg
     2341 tgaagacagt gatcccggtc gcgatgggga cggcggacgg acatcgggtt tgacgcccta
     2401 gggaacgggt ggtgtgttgt ccacatctca tgatacgaat ccggccacaa acagcattgc
     2461 gctttgaaca ccaagatgtc cctgctttct tgggtttcat ggtttcaagg tttcatctaa
     2521 atcgggatcg ggatcgctat cgggttcggc tgtttcacca cgggacactt tcttgggttt
     2581 cagggtttct ggtttcaggg tttcaaggtt tctgggtttc aggtttcaag gtttcatcca
     2641 aatcggggtc ggtatcgcta tcggtatcgg gatcgatcat ttaaccacga acacacggga
     2701 tggcactgct cgggttttca tccacagatt tacacagatt acgcagattt tttgggcatc
     2761 gctcgggttt tttgggaacg ccagagggaa ttcctgattt tcatgaagtt tcatgtgttt
     2821 cagggtttca tccaaatcgg ggtcgggatc gctatcgcta tcgggatcga ttgtttaacc
     2881 acgaacacac gggatggcac tgctcgggtt tttatccaca gatttacaca gattacgcag
     2941 atttttttgg gcatcgctcg gtttttttgg aacgccaggg ggaattcctg attttcatga
     3001 agtttcatgt atttcatggt ttcatccaaa tcgggatcgg gatcgctatc gggatcggga
     3061 tcgctatcgg gatcgggatc gtcgacccgc gacccgcgac attcgaccc
//
LOCUS       DAOSLE010000224         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_28073_length_3041_cov_8.399866, whole genome shotgun sequence.
ACCESSION   DAOSLE010000224 DAOSLE010000000
VERSION     DAOSLE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28073_length_3041_cov_8.399866"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..414)
                     /locus_tag="PK576_10455"
     CDS             complement(169..414)
                     /locus_tag="PK576_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62047.1"
                     /translation="MNAQKKLAFLRTDFVVTFHVIHGADCLVPTGASGDRAFVPDLPI
                     KSQDFPLRGRMYPGFSGLNNIVLIMDICARAAENHVR"
     gene            509..2743
                     /locus_tag="PK576_10460"
     CDS             509..2743
                     /locus_tag="PK576_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62048.1"
                     /translation="MRQVCWIVCCVGMLTGTLQADDSWKGAPGVEGDWNDGANWNSGM
                     VPENEIAFVNNGGYAVIPNGSFLTNLTIAVGQNSGNSGTVRIEPGAMITNTKLLAGNA
                     GTGRGLFLINGGSIDTVTQDFVLGQNSSQATGTVVMTGGEVTAKNVYVGWNGTGYFTL
                     SNGVLRTTSEAYVGTSLKGEFVQEGGTNSFDNGADLSLAKNSNAQGDYTLNNGTLICS
                     DYMYLGYASGARGTLTVNGGSLITSNNVVLGSGKGGSGVITVNGGNANWQALGSNLRV
                     GHESGAFGSLTLNSHTNTFNTVNVGNSSDSTGLVAMTESDITINNGLLFAGATNAMEM
                     NGGRLAVTAGGLTLNGTDSSRFVAHNGAAVSANQVSVGQTGTLRIEDAVLTVTNAAAN
                     AKFEVACGGQLDLNGGTIMCDTFDLPGGTAGERPTVLIREGWFGEKGNLKVYAGHHFV
                     QSGGEVTNAGFYVYGSTTQIVRVEISGGVFSVAPANYAIFNSGTSEFRLKGSAPRVTI
                     PMFSYVGGAPAKHFLLEYTLDKSPAHLAPVNFTDNSAYRCGHLRVALDGGALLLRTNT
                     FALMTGVKSTGFNYTSLPDSNMWSVQELLNTTSSVALASEALKGSLSVGGVMATGPFD
                     PAPMGHVEISNLRTNRLVALNVRLALTPGAKTLEQVVADFADAGFTNSAVESEGDYNV
                     RLTIPAADVPDRSTTPTTWFAWDFTRTPSITNVTTVVTNATLTALKVELEKLPDQGSL
                     IRLF"
BASE COUNT          654 a          822 c          943 g          622 t
ORIGIN      
        1 aggacatgat taacacgatg ttgttcaatc ctgaaaatcc tggatacatg cgcccacgca
       61 gcgggaaatc ctgtcaattt atcgggcatg attttccgct gcgcaggcgc atatatccat
      121 gattaacatg acgttgttca atcctgaaaa tcctgtaatc ctgtcaattt atcggacatg
      181 attttccgct gcgcgggcgc atatatccat gattaacacg atgttgttca atcctgaaaa
      241 tcctggatac atgcgcccgc gcagcgggaa atcctggctc tttataggca agtccggaac
      301 gaacgcgcga tccccggatg cccccgtagg caccagacaa tccgcaccat gaatcacatg
      361 aaacgtcaca acaaaatccg tgcgcaaaaa ggctagtttt ttttgcgcat tcatgccttt
      421 acaggcgcat cggtccgtcc tataatggcc agcagaacgg aaatcggtgt ctttacatgg
      481 cggaaaagca ggcaaacagg aggcggatgt gagacaggtg tgctggattg tgtgttgtgt
      541 cggcatgttg accggaacgc ttcaagcgga tgactcttgg aaaggtgcgc cgggtgtcga
      601 gggcgactgg aatgacggtg ccaactggaa tagcggcatg gtgccggaaa acgaaatcgc
      661 ctttgtcaac aacggcggat atgcggtgat cccgaacggt tcgtttctga cgaacctgac
      721 catcgcggta gggcaaaact cgggtaattc gggcacggtc cggatcgagc cgggtgcgat
      781 gatcacaaac accaagttgt tggccgggaa cgctggcacc ggacgggggt tgttcctgat
      841 caacggcggc agtatcgaca ccgtgaccca ggattttgtc ttgggtcaga actcgtcgca
      901 agccacagga accgtcgtga tgacgggcgg cgaggtcacg gcaaaaaacg tttacgtcgg
      961 ttggaatggc accggctact tcaccctctc gaatggcgtg ctccggacaa cttccgaggc
     1021 ctatgtgggg acttcgctga aaggcgaatt cgtccaagag ggcggaacga acagtttcga
     1081 caatggtgcg gacctctctc tggcgaaaaa ctcgaacgcg caaggtgatt acacgctgaa
     1141 caacggcaca ctgatctgtt cggattatat gtatctgggc tatgcgtccg gagcgcgggg
     1201 caccctgacc gtgaacggcg gcagcctgat cacctcgaac aacgtggtgc tcggtagcgg
     1261 aaagggcggc agcggggtca taaccgtcaa cgggggcaac gccaactggc aggctttggg
     1321 atcgaatctc agggtcggtc atgaatcagg cgcgttcggg agtttgacgc tcaacagcca
     1381 caccaacacc ttcaacacgg tcaacgtggg caattcctcg gattccacgg gtctggtagc
     1441 catgacggag agcgacatca ccatcaacaa cgggctgctg ttcgcgggtg cgacgaacgc
     1501 catggagatg aacggcggca ggctggcggt gacggcaggg gggctgaccc tcaacggaac
     1561 ggactcgagt cgttttgtgg cccacaacgg agcggctgtt tccgcaaatc aggtcagcgt
     1621 cggtcagacc ggtacgctcc ggatcgaaga tgccgtgctg acggtgacca atgcggcggc
     1681 gaacgcgaag ttcgaggtgg catgcggcgg gcagcttgat ttgaatggcg gcacgatcat
     1741 gtgcgacacg tttgatctgc cgggcggcac ggcgggtgag cggcccacgg tgttgatccg
     1801 ggagggctgg ttcggggaga agggcaatct gaaggtttat gccgggcatc actttgtgca
     1861 gagcggcggc gaggtcacca acgcgggctt ttatgtgtac ggctccacga cgcagatcgt
     1921 gcgggtcgag atcagcggcg gcgttttcag cgtggcaccg gccaactacg ccatcttcaa
     1981 cagcggcacc tcggagtttc gcctcaaggg ttccgcgccg cgggtcacga ttccgatgtt
     2041 cagctatgtc gggggggcac cggctaagca ctttctgctg gagtacacgc tcgacaagag
     2101 cccggcgcat ctcgcgcccg tcaactttac ggacaacagc gcctaccgct gcggtcatct
     2161 ccgcgtggcg cttgacggcg gggcgctgct gctgcgcacc aacaccttcg cgctgatgac
     2221 cggggtgaag agcaccggct tcaactacac gtcccttcca gacagcaaca tgtggagcgt
     2281 tcaggaactg ctcaacacca cctcaagcgt ggcgctggca agcgaggcgc tcaagggctc
     2341 gctgagcgta ggcggcgtca tggcgaccgg accgttcgat cctgcgccca tggggcatgt
     2401 ggaaatctcg aatctccgga cgaaccggtt ggtcgcgctc aacgtgcggc tggcgctgac
     2461 gcccggcgcc aagacgctag agcaggtggt ggcggatttc gcggacgccg ggttcacgaa
     2521 ctcggcggtg gagagcgagg gcgactacaa cgtgcgcctc acgatcccgg cggcggatgt
     2581 gccggaccgc tcgaccacgc cgaccacttg gttcgcatgg gacttcacgc gtacgccgag
     2641 catcaccaac gtcaccaccg tggtcaccaa cgccacgctc acggcgctca aagtcgagtt
     2701 ggagaaactg ccggatcagg gcagcctgat cagactcttt tagtgccctt aaaaatctgc
     2761 gcccatctac gtatgaaacg tgaaacctga aaccaaagag cagttgaacc ttcaactgct
     2821 ctttggtttc aggtttcacg tttctggttt ctggtttctg gtttctggtt tctgggtttc
     2881 atggtttcat ctaaatcggg gtcgggatcg ctatcgggat cgggatcgat tattgtgacc
     2941 acgcagtggc tcacaacgac gggggttcaa gggtttctgg tttcatggtt tcatctaaat
     3001 cggggtcggg atcgctatcg ggatcgggat cgattattgt g
//
LOCUS       DAOSLE010000225         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_28213_length_3031_cov_7.142809, whole genome shotgun sequence.
ACCESSION   DAOSLE010000225 DAOSLE010000000
VERSION     DAOSLE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28213_length_3031_cov_7.142809"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..1066
                     /locus_tag="PK576_10465"
     CDS             248..1066
                     /locus_tag="PK576_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE62049.1"
                     /translation="MITREPVYTDAPPNAIAGLGRAIVLGLGDIGRAGSMTAQALAGM
                     PLLLFCARRRRECVRQMFHMGIKTLPVISVVGMFTGMILGLQVGLELRRFNQEIYIGS
                     AVMVSLLREMGPFMTGLILAACVGSSMAAQIGTMTVNDEIAALEIMSIDPVLFLMTPR
                     IAAMVFMTPVLSFYTCVLGVIGGGIVGLTQLNVPWDQYIVMAMDIARLRDLYVGLFKA
                     LLFGIVITGVSCYEGFTTTQGAVGVGQATRRSVISSFLLILILGYIVTRMFYDL"
     gene            1063..1815
                     /locus_tag="PK576_10470"
     CDS             1063..1815
                     /locus_tag="PK576_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE62050.1"
                     /translation="MIRFDHVTKQLGGRLVLDDLSFEIPKGEIFAIVGPSGTGKSVTL
                     KHMVRLMTPTRGAVWIDGTEISAVDGADLARARERFGYLFQGGALLGWMTVEENIALP
                     LREKTRLSEAEIAERVAKALCAVGLEEDGDKFPSEISGGMQKRAGLARAIVREPEIVL
                     YDEPTSGLDPVTARTIDKLIRRLNKELGITSVVVTHDLQGALLSADRIAMLTGGRFVE
                     VSSPHAFVAADQPDVRRFLDAQFISKKGIEGM"
     gene            1864..2976
                     /locus_tag="PK576_10475"
     CDS             1864..2976
                     /locus_tag="PK576_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE62051.1"
                     /translation="MALKRNREVFSEVIVGLFMVAVLGLLAYFTIIISGIDILNGKNK
                     GPVTVLFTDVGGLKVRDSVILRGMTVGSVSEMHLENSGVRVTLLIDGGVRLKEGYRIS
                     VNTGSLLGGNYLLIEEGEGAEVPRETVLRGETPSQWMRDLGVIVANLREATEGSHLRN
                     TLTNLDVAVASIRAAVTRVEEGRGTLGKLFSDDMTLYNDLSNTVASLRSVAVKIDAGE
                     NSLGRLINDDGNVYTNLSASIANLKSISDRLEKGEGTLGKLLSSDAGIYDDLRETVEN
                     LKTVSARLEKGDGTLGKLLKDDNKVYNDLDATVANLREVTDRLVRGEGTLGKLSKEDE
                     LYSDTQGLIRDVRQTVDNFRDTTPISAFASLIMGGL"
BASE COUNT          574 a          801 c         1004 g          652 t
ORIGIN      
        1 aggtttcggg gtttcagggt ttctggtttc agggtttcat ccaaatcggg gtcgggatcg
       61 ggatcgctat cgggatcgat catttaacca caggacactt tcttgggttt cagggtttca
      121 aggtttcagg gtttcgtggg tttcatctgt cgcggccgac aggagtgtct tgcccagtat
      181 cccggcgttt ggtaatctga tgcaacaaaa ggtttaacgc accggcaaag gggataggag
      241 ggttttcgtg atcacgcgcg aaccggttta taccgacgcg ccaccgaatg cgatcgcagg
      301 attgggacgc gcgatcgtgc tggggttggg ggacattggc cgggctggca gcatgacggc
      361 tcaggctttg gccggtatgc ccttgttgct gttctgcgcc cggcgccgcc gcgaatgcgt
      421 ccgccagatg ttccacatgg gtatcaagac cctgcccgta atctcggtcg tcggaatgtt
      481 caccggtatg attctgggac ttcaggtcgg cctcgagctg cggcgcttta atcaggagat
      541 ctacatcggt tcggcagtga tggtctcgtt gttgcgtgag atggggccgt tcatgaccgg
      601 tctgattctg gcggcgtgcg ttggatcctc catggcggcg cagatcggca ccatgacggt
      661 gaatgacgag atcgcggcgc tggagatcat gtccatcgac cctgtgttgt ttctgatgac
      721 gccgcgtatc gcggcgatgg tgttcatgac cccggtgctc tcgttttaca cttgcgtgtt
      781 gggcgtgatc ggcggcggca ttgtgggcct gacgcagttg aatgtgccgt gggatcagta
      841 tatcgtcatg gccatggata tcgcccgctt gcgcgatctg tatgtggggc tgttcaaagc
      901 attgcttttc gggatcgtca tcacgggtgt ctcctgctat gagggcttca ccaccacaca
      961 gggcgcggtc ggtgtgggac aggccacgcg gcgttcggtg atctcctcct ttctgctgat
     1021 cctaatactc gggtacatcg tcacccgcat gttttacgac ctatgatccg ctttgaccat
     1081 gtcaccaaac agttgggggg ccgtctggtg ctcgacgacc tttctttcga aatcccgaag
     1141 ggggagatct tcgcgatcgt cggtccctcg ggcaccggga agagcgtcac actcaaacac
     1201 atggtgcgcc tgatgacccc cacacgcggt gcggtgtgga tcgacgggac ggaaatcagt
     1261 gccgtggacg gggcggattt ggcgcgcgcg cgagaacgct tcggctacct gttccaaggc
     1321 ggcgcgctgt tgggctggat gaccgtcgag gagaacatcg ccctgccgtt gcgtgaaaag
     1381 acgcgccttt ccgaagcgga gatcgcggag cgggtggcca aggccttgtg cgcggtcggg
     1441 ttggaggagg acggcgacaa atttcccagt gagatttccg gcgggatgca gaagcgtgcc
     1501 gggttggcgc gggccatcgt gcgtgaaccg gagatcgtgc tctatgatga gcccacttcc
     1561 ggtctcgatc cggtgaccgc gcgcacgatc gacaaactga tccggcggct caacaaggag
     1621 ctgggcatca ccagcgtggt ggtgacgcat gatttgcagg gtgccttgtt gagtgcggac
     1681 cgcatcgcca tgctgacggg cgggcggttt gtggaggtca gttcgccgca cgcgtttgtg
     1741 gccgcggacc agccggatgt ccggcgtttt ctggacgccc agtttatttc aaaaaaaggg
     1801 attgagggga tgtgaacgcg cgggtccgtc acgcacggtc cgtaaccgca aaaggaggaa
     1861 cgtatggctt tgaaacgcaa ccgcgaggtc ttttccgagg tgattgtcgg cctgtttatg
     1921 gtcgcggtcc tcggactgct ggcctatttc accatcatca tctccggtat cgatatcctg
     1981 aacggcaaga acaaggggcc ggtcacggtg ctgtttaccg atgtcggcgg attgaaggtg
     2041 cgcgacagcg tgattctgcg cgggatgacg gtggggtccg tgagcgagat gcacttggag
     2101 aatagcgggg tgcgggtcac gttgctgatc gatggcgggg tgcgcttgaa ggaggggtac
     2161 cgcatctcgg tcaacaccgg atctttgctg ggcggcaact acctgctgat cgaagaaggg
     2221 gagggtgccg aagtgccgcg ggaaaccgtg ttgcggggtg agacgccttc ccagtggatg
     2281 cgcgatctgg gggtaattgt ggccaatctg cgcgaggcca ccgaaggcag ccacctccgc
     2341 aacaccctca ccaatctgga cgttgccgtg gcctcgatcc gcgcggcggt cacgcgtgtc
     2401 gaagagggcc ggggtacgct cggcaagctg ttttccgatg acatgacgct ctacaacgac
     2461 ctctccaaca ctgttgcgag cctgcgttcg gtggcggtca aaatcgatgc cggcgagaac
     2521 tcgctaggcc ggctgatcaa cgacgacggc aacgtctaca ccaacctcag cgcctcgatc
     2581 gcgaacctca agtcgatttc cgaccggctg gaaaaggggg agggcacact cggcaaactg
     2641 ctctcttcgg atgccggaat atatgacgac ctgcgcgaga cggttgagaa cttgaagacg
     2701 gtttcggcgc gtttggagaa gggggacggc acgctcggca agttgctgaa agacgacaac
     2761 aaagtctaca acgacctcga cgcgacagtg gcgaatctgc gcgaggtgac cgatcggctc
     2821 gtccggggcg aaggcacgct cggcaaactt tccaaagagg atgaactgta ctccgacacg
     2881 cagggactca tccgggatgt gcgacaaacc gttgataact ttcgcgacac gaccccgatc
     2941 agcgcattcg ccagtctgat catgggcgga ttataaaccg gagaaccccg tatgctgtac
     3001 acacttggag aaatggcgga gaagttgaac c
//
LOCUS       DAOSLE010000226         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_28473_length_3010_cov_8.333672, whole genome shotgun sequence.
ACCESSION   DAOSLE010000226 DAOSLE010000000
VERSION     DAOSLE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28473_length_3010_cov_8.333672"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK576_10480"
     CDS             <1..252
                     /locus_tag="PK576_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE62052.1"
                     /translation="SLWVDGNYKAYRLRRRRERQASVDGRKGEHDRNLVSRAEIESEI
                     SAAGLDIIGHVDFLKYWEKWRTYVLAVRQGAINSRRTHA"
     gene            complement(317..1342)
                     /locus_tag="PK576_10485"
     CDS             complement(317..1342)
                     /locus_tag="PK576_10485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE62053.1"
                     /translation="MRILMVDNMQIRQYGNLKMGPGRKLVCAAIRDNHRLAEFSDRDV
                     ARFLAPLGIRSIGARLANRKLIQTALNFKPDILFIGHCDYIRNRSLEAIRAALPSIRM
                     AHFNIDPIWMDWHIQQIRERMPVTDAIFVTTGGEALKAFCTGKNVVAYMPNPSDPAME
                     ICDNSQKQTFRHDLLFCGKEIAGDDRNAFLRLLERDLSGKLRFGIYGMFGYPPVWGAR
                     YEETLAESKMALNLNRREEWPLYSSDRIAHLMGNGILTFLSDRGGMQRFFSEREAVFF
                     HEHGDLCEKILYYQEHDDERQAVAAAGRARYHQLFDSRRTLKFMIETLLNEPYSEDYE
                     WADHVFR"
     gene            complement(1369..1950)
                     /locus_tag="PK576_10490"
     CDS             complement(1369..1950)
                     /locus_tag="PK576_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE62054.1"
                     /translation="MAKACDLRKGSLVGINGLPHVVEDLKVSTPSARGAASIYRFRFR
                     NLVTKTKQDVSCKGEEPFDEIDFERRAVQYLYREGDLYTFMDAEDFSQFSLSQDLIEE
                     QIPFLIEEMEGLFALVSEGKVLTVELPQKVALRIAACDPVMKGASVTSRSKPATCDTG
                     LVVQVPEYLEAGETILVDTATGAYLSRATADRM"
     gene            2086..2619
                     /locus_tag="PK576_10495"
     CDS             2086..2619
                     /locus_tag="PK576_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOE62055.1"
                     /translation="MRKTGANRVIVITGEGKGKTTAALGLVLRAVGHGLGVCVLHFIK
                     NRRDTGEARALAGLPGVEAHQCGRGFVRERSGPVFEAHARAAHAGLRLAAAKLRDPAF
                     GMVVLDEICGAVALGLLPVEAVCAAVAGAAPGTIIVLTGRDAAPELVELADTVSGITC
                     VRHGLEAGWPAQAGVEF"
     gene            2939..3010
                     /locus_tag="PK576_10500"
     tRNA            2939..3010
                     /locus_tag="PK576_10500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2972..2974,aa:Thr,seq:ggt)
BASE COUNT          562 a          815 c         1040 g          593 t
ORIGIN      
        1 tcactttggg tcgatggcaa ttacaaggct tatcggctca ggcgaagacg ggaacgccag
       61 gcctccgttg atgggcgaaa aggagagcat gatcgcaacc ttgtgtcgcg agcggagatt
      121 gaaagcgaaa tcagtgctgc ggggctcgac attattggac atgtcgactt cctcaagtat
      181 tgggagaaat ggcgaactta tgttctggcg gtaaggcagg gcgctataaa cagccgtcgg
      241 acacacgcat gatggccggc cgtcccgagg ccaaggacgg gaacactttg ccggcgatga
      301 cgcggtcgcg tgcgggtcag cggaagacgt gatccgccca ctcgtagtct tccgagtagg
      361 gttcgttcaa cagggtttcg atcatgaact tcagggtgcg ccggctgtcg aagagctgat
      421 gataacgtgc ccggcccgcg gcggcgacgg cctgccgttc gtcgtcatgc tcctgatagt
      481 agaggatttt ttcgcacagg tcgccgtgct catggaagaa gaccgcctcg cgttcgctga
      541 agaagcgctg catgccgccc cggtcgctga gaaaggtcag gatgccgttg cccatcaggt
      601 gcgcgatgcg gtctgaggag tagagcggcc actcctcccg gcggttcagg ttgagggcca
      661 ttttactttc ggcgagggtc tcttcgtaac gggcacccca gaccggcggg taaccgaaca
      721 tgccgtagat accgaaccgc agttttccgg acaggtcacg ctccagaaga cgcaggaacg
      781 cgttgcggtc atctccggcg atctccttgc cgcagaagag caggtcatgc cggaacgtct
      841 gtttttgcga gttgtcgcaa atctccatgg cgggatcgga ggggttgggc atgtaggcca
      901 caacgttttt gccggtacag aacgctttaa gcgcctcgcc gccggtggtg acgaaaatcg
      961 cgtcggtgac cggcatccgc tcgcggatct gctggatatg ccaatccatc cagatggggt
     1021 cgatgttgaa atgcgccatg cggatggagg gcagggccgc acggatcgcc tccagggacc
     1081 ggttacggat gtagtcgcaa tggccgatga agaggatgtc cggtttgaag ttcagggcgg
     1141 tctggatgag ctttcggttt gccaggcgcg cgccgatgct gcggatgcca agcggcgcaa
     1201 ggaagcgggc gacgtcacgg tcgctgaatt cggccagccg gtggttgtcg cggatggcgg
     1261 cgcagaccag cttacgaccc ggccccattt taaggttgcc gtactgtcgg atctgcatgt
     1321 tgtcgaccat caggatacgc atcggggctc cttggtgtgg gggttacgtc acatccggtc
     1381 ggcggtggcg cgcgagagat aagcgccggt ggcggtgtcc acgaggatcg tctcgcccgc
     1441 ctccagatac tcgggcacct gcaccaccag gccggtatca caggtggcgg gtttgctgcg
     1501 tgaggtgacg gacgcgccct tcatcaccgg gtcacaggcg gcgatgcgca gcgcgacctt
     1561 ctgcggcagc tcgacggtga gcaccttgcc ttcggatacc agggcaaaga gcccttccat
     1621 ctcctcgatt agaaagggga tctgttcttc gatgaggtcc tgcgacaggg agaactggga
     1681 gaagtcttcg gcgtccatga aggtatagag gtcgccttca cggtagaggt actgcacggc
     1741 acgacgctcg aaatcgatct cgtcgaacgg ctcctcgcct ttgcaggaga catcctgttt
     1801 tgttttggtg acaaggttgc ggaatcggaa ccggtaaatc gaggcggcgc cgcgggcgct
     1861 gggagtggag accttcaggt cttcgacaac gtggggcagg ccgttgatcc cgacgaggct
     1921 gccttttctc aggtcacaag ctttggccat aagccttttc tccgaaaagg aaaaaagtat
     1981 agcgtgttgg gttcggcctg tcgagcgcgg gaaatccgcg cttttcgcgt gtaacggaaa
     2041 acgctatcct ttccttttcc ccaacgcggg aacgtaagga ggggcatgcg gaagaccggg
     2101 gcgaatcggg tgatcgtgat taccggcgaa ggcaagggca agaccacggc cgcgctgggc
     2161 ttggtgctgc gtgcggtggg ccacgggctg ggcgtgtgcg tcctgcactt catcaaaaac
     2221 cgccgggaca ccggggaggc gcgggccctg gccggattgc cgggggtgga ggcgcaccaa
     2281 tgcgggcggg ggttcgtccg ggaacggtcg ggtcctgtct tcgaggcgca cgcgcgcgcc
     2341 gcgcacgcgg gattacggct cgccgccgcc aagttgcgcg atcccgcatt cgggatggtg
     2401 gtgctcgatg agatctgcgg ggcggtcgcg ctcggcctgc tgccggttga ggcggtctgt
     2461 gcggcggtgg ccggagcggc gcccggaacg atcattgtgc tgaccgggcg cgacgccgcg
     2521 ccggagttgg tggaactggc ggacacggtc agcgggatca cctgtgtccg gcacggtctg
     2581 gaggcgggat ggcccgccca ggccggcgtg gagttctgag ccgcggcacg ggcgtcacgc
     2641 ccgtgaaaat catgggcaaa gatgcccatg ccgcgcatgc agggccagaa acgcataaac
     2701 ctcgcgcatc cctgccgctg accgaatgcg aaaaactttt tcgcgcggat tgacaggagg
     2761 gcctcctttg ggtaatctaa acgagttttc tttttttccg ggactcggtt ccgggacaag
     2821 ggaaaacaga cattggcaag gttaaaggga ctccagcacg atggccttaa agcccccttg
     2881 gggctggcgg gcaattgggc aaggggttct ttgccccctt tggggggttg gggacgaggc
     2941 caacatagct cagtcggtag agcacttcct tggtaaggaa gaggtcgtca gttcgattct
     3001 gattgttggc
//
LOCUS       DAOSLE010000227         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_28484_length_3009_cov_8.236290, whole genome shotgun sequence.
ACCESSION   DAOSLE010000227 DAOSLE010000000
VERSION     DAOSLE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28484_length_3009_cov_8.236290"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2460
                     /locus_tag="PK576_10505"
     CDS             247..2460
                     /locus_tag="PK576_10505"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOE62056.1"
                     /translation="MRRWICFFGWCGAAALQSAALTPADLMCELLAKPGLVAVTDATP
                     DFSWGFQDVRPGDFQSAYQIQVASGTAFLKTDQPDLWDSGKVASGQSLYIAYAGKALP
                     TDAEVCWRVRVWDGRGKEGPWSHTYSFKTAPVLSGDSALRYPLAQEWVKPVRVVTNAQ
                     GRVLVDFGRAAFGWVELVPPHELRRGGPFTLHLGEKLKGDSVDAKPGGTIRYAKVNGA
                     LTSPNVYRVPLVADKRNTSGGKEGAAILLPPEFGVVMPFRYVEIEECPYTVTPDTIRQ
                     IAVFYPFDMEAARFVSSDAVLDRVFDFCKYSIKATTFAGVYVDGDRERIPYEADAYIN
                     QLCHYCTDREFTLARYSHEYLMAHPTWPTEWKQHSVMMAWADWMYTGNTESLERMYDR
                     LKAEKTLEKNARASDGLLVTGGPSAPMESGLRDITDWPVGERDGFVFKPVNAVVNAFY
                     CLNLRQMAEMAAALDKTADAEAYRAKHERAVKAFNALFFHAERGCYVDGEGATHASLH
                     ANMLPLAFGLVPEAERGRVTRFVVSRGMACSVYGAQYLLEALFEADQDDTAIHLMTRD
                     ELRSWVNMMKAGSTITMEAWDIMVKPNLDWNHAWGAVPANIIPRFVMGVRPIEPGFGK
                     ILIRPQVGSLEAIEGYVPTVRGTVRVGVRQKPGTQYAVTVEIPVNTTARVELPWCEGA
                     RLRVNGKKVAARVEGGRFVVDDVTSGRHTLVWQCGEEAACGSSFGRRVRRIFGGG"
     gene            2409..2813
                     /locus_tag="PK576_10510"
     CDS             2409..2813
                     /locus_tag="PK576_10510"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOE62057.1"
                     /translation="MRFEFRQASPEDLRRWLTFLGAALLIALGVVALDAAGVRLCLFR
                     RLTGLPCLTCGGTRAFAALATGDVTGAFRQQPLVSALLTVGTAAGAVHSLLLARGRHL
                     TLRTSASERRLLLLAAVILAAANWVYLLWRGA"
BASE COUNT          538 a          858 c         1037 g          576 t
ORIGIN      
        1 gtctcgggac gccctcggtg tccagtattt gacggatcgt caccgcctca aaaaaaccta
       61 cggcgtcttt tctatttgac ggaccacgga tcaggacgca ttcctgctct cccgcagatt
      121 ccgattgccc gcaaagcccc aaacctgtta cgttgggttc atcccttcat ttagaaaacc
      181 cccatgcgaa tgtggcgggt ttcaaggccg gcatgctgct ggcaccgtga gcggaaagga
      241 aacgcgatgc gacgttggat ctgttttttc ggatggtgcg gggcggcggc gctgcaaagc
      301 gccgcgctca ccccggctga cctgatgtgc gaactgttgg ccaagccggg gctggtcgcc
      361 gtgaccgacg cgacgccgga tttttcctgg gggtttcagg acgtgcgccc cggcgacttc
      421 caaagcgcct accagattca ggtggcgtca ggaaccgcgt ttctgaaaac cgatcaaccg
      481 gatctctggg attcgggcaa agtcgcctcg ggtcagtcgc tttacatcgc ctacgcgggc
      541 aaggcgctgc ccaccgacgc tgaggtgtgc tggcgggtgc gcgtgtggga cggccgcggc
      601 aaggaagggc cttggtcgca tacgtactcc ttcaaaaccg cgcccgtatt gagtggagat
      661 tcggcgttgc ggtatccgct cgcgcaagag tgggtgaagc cggtccgcgt ggtcaccaac
      721 gcgcaggggc gcgtgctggt cgatttcgga cgggcggcct tcggttgggt cgagctggtt
      781 ccgccgcacg aactgcggcg gggcggcccc ttcacgctgc atctcggcga aaagctgaag
      841 ggcgacagcg ttgacgccaa accgggcggg acgatccgtt acgcgaaggt gaatggggcg
      901 ttgaccagtc cgaatgtcta ccgcgtgccg ttggtcgccg acaaacgcaa cacgagcggc
      961 ggcaaagagg gtgcggcgat tttgctgccg cctgagttcg gcgtggtgat gccgttccgc
     1021 tatgtcgaga tcgaggagtg cccgtacacg gtgacgcccg acacgatccg ccagattgcg
     1081 gtgttctatc cgttcgacat ggaggcggcg cgttttgttt cgagcgatgc cgtgctcgac
     1141 cgcgtgttcg atttctgcaa gtacagcatc aaggccacga cctttgcggg cgtgtatgtg
     1201 gacggtgacc gcgagcgcat cccgtatgag gccgatgcct atatcaacca actctgccac
     1261 tactgcaccg atcgcgaatt cacactggcg cgctacagcc acgagtatct gatggcgcat
     1321 ccgacatggc cgacggaatg gaagcagcac tcggtgatga tggcgtgggc ggattggatg
     1381 tacaccggca acacagagtc gttggagcgc atgtatgacc gcctcaaagc cgaaaagacg
     1441 ctggagaaaa acgcgcgcgc ctcggacggg ttactggtca ccggcggccc ctccgcgccg
     1501 atggaaagcg ggctgcgtga catcacggac tggccggtcg gcgagcgtga cgggtttgtg
     1561 ttcaaacccg tcaatgccgt ggtcaacgcc ttctactgcc tgaacctgcg tcagatggcg
     1621 gagatggccg cggcgctgga taagacggcg gatgccgagg cgtaccgtgc gaaacacgag
     1681 cgggccgtca aggcgttcaa cgcgctcttc ttccatgccg agcgcggctg ctacgtggat
     1741 ggcgaaggcg ccacccacgc ctcgctgcac gccaacatgc tgcccttggc gttcggcttg
     1801 gtcccggagg cggaacgcgg acgggtgaca cgctttgttg tctcgcgcgg gatggcgtgc
     1861 agcgtgtatg gcgcgcagta tctgctcgag gcgctcttcg aggccgatca ggatgatacc
     1921 gcgatccacc tgatgacgcg cgacgagctg cgtagctggg tcaacatgat gaaggccggc
     1981 tcgaccatca cgatggaggc ctgggacatc atggttaagc cgaatctgga ctggaaccac
     2041 gcatggggag cggtgcctgc caacatcatc ccccgctttg tcatgggcgt ccggccgatc
     2101 gagcccggat tcggaaaaat cctgatccgg ccgcaggtcg gttcgctgga ggcgatcgaa
     2161 ggctatgtgc cgaccgtacg gggcaccgtg cgggtgggcg tgcgccaaaa accggggaca
     2221 cagtacgccg taacggtaga gatccctgtc aacacgaccg cgcgcgtcga gttgccgtgg
     2281 tgtgaaggcg cgaggctccg ggtgaacggc aagaaggtgg cggcacgggt ggaaggcgga
     2341 cgctttgtgg tggacgacgt gacgtcggga cgacatacgc tggtatggca gtgcggggag
     2401 gaggctgcat gcggttcgag tttcggcagg cgagtccgga ggatcttcgg cggtggctga
     2461 cgtttctggg cgccgctctt ctgatcgcgc tgggtgtggt cgcgctggac gccgccgggg
     2521 tgcggctttg tctctttcgc cgtttaaccg gcctgccctg tctgacctgc ggcggcacgc
     2581 gcgcgttcgc ggcgctagcg accggtgatg tgaccggcgc gttccgtcag cagccgctcg
     2641 tgagcgcact gctgacggtg gggacggccg ccggtgccgt ccacagcctg ttgctggccc
     2701 ggggcagaca tctgacattg cggacatccg cgagcgaacg aaggctattg ctgttggccg
     2761 ccgttattct ggcggcggcc aactgggtat acctgctctg gcgcggcgca tgaaaccgtt
     2821 gccgggaagg ggcaggcgga gtttggattt aggatttagg atttaggatt taggagttag
     2881 cccgccgccc gcgcttcgca cgggaatgta actgaggata acgttgatgt gggttatagg
     2941 caaagaggga ggatttagcc cgccgcgcgg ctcagccgcg aaaaaacctt ggaatacgtt
     3001 taggacatg
//
LOCUS       DAOSLE010000228         2990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_28729_length_2990_cov_8.058262, whole genome shotgun sequence.
ACCESSION   DAOSLE010000228 DAOSLE010000000
VERSION     DAOSLE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28729_length_2990_cov_8.058262"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..>2990
                     /locus_tag="PK576_10515"
     CDS             80..>2990
                     /locus_tag="PK576_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62058.1"
                     /translation="MKSRLSFDSCFISAWTFAVAAFCFQMGAQAQTSVSLYKGPITGS
                     SPENPVDFSVTSYWDPAGMPSGTTAEAKFAKATMGTLTEQMYVALPNDLTLLTLRAGG
                     GKIINFIGENVSLGTLHYHDYLGYASVLYATAHAFGNDISSVCGAFAGDVVNAISGKM
                     VFAGGEVEHHHHCYANSDSETILNALPTNFWTHGSANLHLYASRQCGEVTGLWKATKD
                     SKHLKYVGASAIHVVAPGAKVTCPGIVPTGAYVRRWFSNAKTIELSAAALDNADSTNL
                     TFHAYNPSVRQDVVTWQKQGGAQNAILNKWSTGQNAVLEVDTLQGGSADAITFDAEGN
                     CYPWRVIIHNGKILSAVNFKYADIEFADNPSGVRGLPNSAVSVTSSSKSSTVTVPGGE
                     NTAYFKTFGPIAAGGGLIKKGAGALVAKLGDDLFGSLEVAEGSVSFTNAVDTAKIYEL
                     GSLTLGAGTVFNVDGGVTLNVKMISVGTGARITGTGTIAFPNLSMIPEGVIASSVKIG
                     IAGVSGGLVHGEIVPAVVGDPAFWLDTAAADSLVTEENGAVTNVVRWNDCRKASPDDG
                     YMFATNCWAKPTLANMLIQNYTHRSEGARINSFNRNYVRIVANPASLAHCEALVWSRK
                     LTGIKSVFLVMSAADGGGCVLGNTAKLGDFGRGATGQAAIFYSGTDASPEKVYKAPMY
                     INGVKVVNHFTFGPYTGFPNNWRPFLFEVHPTADGGEADAFGMQGTNRLDLAGGQRIF
                     ECILYTNELTRTEQAQVADYLTRKWFNACYGGAYALDEAEETIDATTFDRLDIVSGDY
                     AVEAITGTSFVKTGAGGMYVASLTGGTDLTIPEGSMVISSAKAASFANLPVDDSGVYM
                     HLDANNLEGSTMSEIDGKGECVKIWKSLRGATLRTATNFSTNHPALKTTGGSARMKDG
                     MKYVDLGEFREVPTTTGYGTVNANCGETYHFVKSDGIRAMFMVFGGTGPLVGNNGI"
BASE COUNT          594 a          900 c          932 g          564 t
ORIGIN      
        1 acgccaacgc caaaatcgcg cacaatttta atgatcctga taaaagggtt ttgtgaaaat
       61 cacctccgag gagtcttgta tgaaaagcag gctgagcttt gattcttgtt tcatctccgc
      121 ttggacattt gccgtggcgg cgttctgttt ccagatgggt gctcaggcgc aaacctccgt
      181 gagtctctac aaggggccga tcaccggaag ttcgcctgag aatccggtgg acttttcggt
      241 gacctcgtac tgggatccgg cgggcatgcc gtccggcaca accgccgagg cgaagttcgc
      301 gaaggcgacg atgggcacgc tcaccgagca gatgtacgtg gcgttgccga atgacctcac
      361 gcttttgacg ctgcgtgccg gcggcgggaa gatcatcaac ttcatcggcg agaacgtcag
      421 cctcggcaca ctccactatc acgactacct cggctacgcc tcggtgctct acgcgacggc
      481 ccacgccttc gggaacgata tctccagtgt gtgcggcgcc ttcgcgggcg atgtcgtcaa
      541 tgcgatcagc ggcaagatgg tgttcgccgg cggcgaggtc gagcaccatc accactgcta
      601 cgccaattcc gattccgaga cgatcctcaa cgccctgccg accaacttct ggacgcacgg
      661 ctctgccaac ctccatctct atgcgtcccg ccagtgtggc gaggtgaccg ggctttggaa
      721 ggcgacgaag gattcaaagc acctgaagta tgtcggcgct tctgcgatcc acgtggtggc
      781 gccgggcgcg aaggtgacct gcccggggat cgtgccgacg ggtgcctacg tccgccgctg
      841 gttctctaac gcgaagacga tcgaactttc tgcggcggcg ctggacaacg ccgattcgac
      901 caacttgacc ttccatgcct acaatccgtc cgttcgccaa gacgtcgtga cctggcagaa
      961 gcagggggga gcccaaaatg cgattctcaa caagtggagc accgggcaaa atgcggttct
     1021 ggaggttgat acgttgcagg gcggcagcgc ggacgcgatt acgttcgacg cggaggggaa
     1081 ctgctatccc tggagggtca ttatccacaa tggcaagatc ttgagtgcgg ttaatttcaa
     1141 gtatgcggat atcgaattcg ccgacaatcc ctccggtgtg cgcggtcttc cgaattccgc
     1201 cgtcagcgtc acctcctcgt ccaagtcctc gaccgtcacc gtgccgggcg gggagaacac
     1261 ggcctatttc aagacattcg gccccattgc cgcaggcgga gggctcatca aaaagggggc
     1321 gggcgcgctc gtcgcgaaac tcggcgacga tctttttggt tcgcttgaag tcgcggaagg
     1381 ttcggtctcc ttcaccaacg cggtcgacac cgccaagatc tacgagctcg gctcattgac
     1441 cctcggcgca gggacggtct ttaacgtcga cgggggcgtg acgcttaacg tgaagatgat
     1501 ttccgtcggc acgggggcgc ggattaccgg cacgggcacg attgccttcc ccaacctctc
     1561 catgatcccc gagggcgtga tcgcctcttc ggtgaagatc gggatcgcgg gcgtcagcgg
     1621 cggtctcgta cacggcgaga tcgtccccgc cgtggtgggc gatccggcgt tttggctgga
     1681 cacggctgcg gcagattcgc ttgtcacaga ggagaacggc gctgtgacga acgtcgtgcg
     1741 ctggaacgac tgccgcaagg catcgccgga tgacggctac atgttcgcga cgaactgctg
     1801 ggcaaaacca acgctcgcga acatgctgat tcagaattac actcaccggt cagagggagc
     1861 ccggatcaac agtttcaacc gcaactacgt gaggatcgtc gcgaatcccg cctcgctggc
     1921 ccactgcgag gcgctcgtgt ggagccggaa gctgacgggg atcaagtccg ttttcctcgt
     1981 gatgagcgcg gcggacggcg gcggatgcgt tctcggcaac acggccaagc tcggcgactt
     2041 cgggcgcggc gccacggggc aggccgccat tttctattcc ggaaccgatg cctcgccgga
     2101 gaaggtgtac aaggcgccga tgtacataaa cggcgtcaag gtcgtcaacc atttcacgtt
     2161 cggtccgtac acgggtttcc cgaacaactg gcgccccttc ctcttcgagg tgcatccgac
     2221 ggcggacggc ggcgaggcgg acgccttcgg catgcagggg accaaccggc tcgatctcgc
     2281 gggcggacag cggatcttcg agtgtatcct ctacacgaac gagctcaccc gcaccgagca
     2341 ggcgcaggtg gccgactacc tcacgcgcaa gtggttcaac gcctgctacg gcggcgcgta
     2401 cgcgctggac gaggcggagg agacaatcga cgcgacgacg ttcgaccgtc tcgacatcgt
     2461 gtccggcgat tacgcggtgg aggcgattac cggcacctct ttcgtgaaaa ccggcgcggg
     2521 cgggatgtat gtcgcctcgc tcaccggcgg cacggatctc acaatcccgg aaggttcgat
     2581 ggtgatctcc tccgcgaagg cggcgagctt cgcgaacctg ccggtggacg acagcggcgt
     2641 ctacatgcac ctcgacgcga acaacctcga aggttccaca atgagcgaga tcgacggcaa
     2701 gggtgaatgt gtgaaaatct ggaagagtct ccgcggcgcc acgctccgca cggcaacgaa
     2761 tttctccacc aatcacccgg cgctgaagac gacaggcggc tccgcgcgca tgaaagacgg
     2821 catgaagtac gttgacctcg gcgaattccg ggaagtgccg acgacaaccg gatacggtac
     2881 cgtgaacgcc aattgcggtg agacgtatca cttcgtgaag tccgacggca tccgtgcgat
     2941 gttcatggtg ttcggcggca ccggtcccct tgtagggaat aatggcattt
//
LOCUS       DAOSLE010000229         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_29350_length_2938_cov_7.842178, whole genome shotgun sequence.
ACCESSION   DAOSLE010000229 DAOSLE010000000
VERSION     DAOSLE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29350_length_2938_cov_7.842178"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PK576_10520"
     CDS             complement(<1..532)
                     /locus_tag="PK576_10520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE62059.1"
                     /translation="MMNAKDALPHYKFAEKYDQAHALTYFNKHNSGLGRRLFTWREVG
                     MARKALVLAGRPHSVLDLPCGTGRFWSMLAEEKSRKIFVADNSRAMIDTGLALRPREI
                     VSRIEKSFQCSAFDTALPENFVENVFCMRLLHHISRSEDRIRMLREFARVSSRSVLVS
                     LWVDGNYKAYRLRRRRE"
     gene            756..1880
                     /locus_tag="PK576_10525"
     CDS             756..1880
                     /locus_tag="PK576_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62060.1"
                     /translation="MSDLSNRSLPFRTWLDQNRFRYAVLWNLDAPTAKNPLCPGDRHV
                     IVLEDRAIQPADQHWGCRWRHPRNPYRVYSVNGENGTDYLGHPFLPTALAENLLAHTQ
                     QDASGLPMPAPRDRYDALLYHLAYHLAETSGADAEDPLAFRNSPWHTQVRSAGEPLGL
                     PYDLSLLDMHRWLKEHGYGFSAARRTAYLQHQFKNRFKSAFYAFALCMEMENQGEMNL
                     FVLRSKVIRHGFRQRLLDEIARHYTILAVRDIPWLTRLRKARHMRGNKWRWGGWPVVA
                     VVVFDPAPIWRTRAERAQEHSFVLNSRQFFKRELREQIVQEGGLYHKHNAMHTTDNEA
                     EAIGHLHLFFPPEAMQEVFDRLAAARSALDPSAPPNAFAP"
     gene            complement(1983..2768)
                     /locus_tag="PK576_10530"
     CDS             complement(1983..2768)
                     /locus_tag="PK576_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62061.1"
                     /translation="MRVLKRWWLRSRAFRAAQPELEACRATMERLFGGPVTWRRTGGC
                     GRDVVCLVSCGGKPVGVLRRVAPVAVRASGEPRKEQPAVPLGPEEKIKREWQAYAVGA
                     PHGLTPVPLWRAERILICAYVDGVPLKKEAERNGTYLDVAAAALPHIARLHRLGVTHL
                     DMSLSNILAEETGRRVFVDFEYGPAAGLTFEQQCLHDYLRLLESVWKFLDPPQRGRVG
                     RVWDEALRAHAPDQVRAAEIAPLRPALTRILAAPEMRVFWKGL"
BASE COUNT          627 a          958 c          756 g          597 t
ORIGIN      
        1 gttcccgtct tcgcctgagc cgataagcct tgtaattgcc atcgacccaa agtgacacga
       61 gcacggatct tgaactcact cgcgcaaact cccgaagcat cctgatccgg tcttcgcttc
      121 ttgaaatatg gtgcagcaaa cgcatgcaaa aaacattctc gacgaagttt tcaggcaatg
      181 cggtgtcgaa ggctgaacac tggaacgatt tttcaatgcg cgatacgatc tctcgcgggc
      241 gcaaagccaa tcctgtgtct atcatcgccc ggctattgtc agcgacgaat atcttacggc
      301 tcttctcctc ggcaagcatc gaccaaaaac ggcctgttcc acaaggcaag tcgagtacag
      361 aatgggggcg gcccgccaaa acaagcgctt tccgcgccat gccaacttca cgccaagtaa
      421 acagacgccg tccaagacca gaattgtgct tgttgaaata cgttagcgca tgcgcctgat
      481 catacttttc ggcaaacttg tagtgtggca atgcgtcctt tgcattcatc attttcactc
      541 cagtcttggc tcctctgaat gttggtaatg gtaacaaacc cttaaacaga cctaaagagg
      601 catatgcaac attaacaaat tgtaatctta ctgatgaaac gcatcacggt tcatgtttgt
      661 ttgatcatgg atccgaagtc tcgcaggctt gcgttcctta atcgatgcgg ctatgattgc
      721 cggaattcat atcccgacag acctgacgtg acagcatgag tgacctttcg aaccgatccc
      781 ttccttttcg aacctggctt gaccagaacc gtttccgtta tgcggtattg tggaatctcg
      841 acgcgcccac ggcaaaaaac cccctctgcc cgggggaccg gcacgtgatc gtattggaag
      901 accgcgcgat ccagcctgca gaccagcact ggggctgccg atggcggcac ccccggaacc
      961 cgtatcgggt gtatagcgtg aacggggaaa acggcaccga ttatctcggg catccttttc
     1021 tgcctaccgc cctcgccgag aacctgttgg ctcacaccca acaggacgcc tcaggcctgc
     1081 cgatgcctgc tccccgcgac cggtatgacg cgctcctcta tcatctcgct tatcatctgg
     1141 ccgagaccag cggtgcggac gcggaagacc cgctcgcctt ccgtaacagc ccctggcaca
     1201 cccaagtccg atccgcggga gaaccgctcg gtttgcctta cgacctttcg ttgttagata
     1261 tgcaccgctg gttgaaggaa catggctacg gctttagcgc ggcccgtcga acggcatacc
     1321 tgcagcatca gttcaaaaac cgcttcaaga gcgcttttta cgctttcgcg ttgtgtatgg
     1381 agatggagaa ccagggggag atgaatcttt ttgtcctgcg gtcgaaagtc attcgccacg
     1441 gtttccgcca acgcctcttg gatgaaatcg cccgccacta cacgatcttg gccgtgcgtg
     1501 atattccctg gctcacccgg ctccggaaag cccgccacat gcggggaaac aaatggcggt
     1561 ggggcggctg gcccgttgtg gcggttgtgg tgtttgatcc cgcgccgatc tggcgcacgc
     1621 gggcggaacg cgctcaagaa cattcgttcg tgctgaacag ccggcaattc ttcaagcggg
     1681 aacttcgcga acagatcgtt caggaaggcg ggctgtatca caaacacaac gccatgcaca
     1741 ccacggataa tgaggcggaa gcgatcggcc atctgcacct gttttttcct ccggaggcga
     1801 tgcaggaggt tttcgaccga ttggcggcgg cgcgctccgc gctggatccg tcggcccctc
     1861 caaacgcgtt cgcgccgtga cccgcgcgcg ggaagtggcg atcccgttcc aaaacggggg
     1921 gccgtcgcca gcccttcccg actgaacgca acgcatccgc ccgcacacct cgtcccgcga
     1981 catcagagtc ccttccagaa aacccgcatc tcaggcgcgg cgagaatacg cgtcaatgcg
     2041 ggccgcaagg gcgcgatctc ggccgcgcgg acctgatccg gcgcatgcgc acggagcgcc
     2101 tcgtcccaaa cgcgcccgac acggcctcgc tgcgggggat ccagaaactt ccagacgctt
     2161 tccaacaacc gaaggtagtc atgcaggcac tgctgttcga acgtcagccc cgctgcgggg
     2221 ccatactcaa agtccacgaa cacccgccgt cccgtttctt ccgccagaat gttcgaaaga
     2281 ctcatgtcca gatgcgtaac acccaaccgg tgcaaccgcg caatgtgcgg caaagccgcc
     2341 gccgccacat ccagataggt tccattgcgc tcggcctcct ttttgagcgg caccccatcc
     2401 acgtacgcac atatcaggat ccgctccgcg cgccacagcg gtacgggagt caacccgtgc
     2461 ggcgcaccga ccgcgtaagc ctgccactcg cgcttgatct tttcctccgg accgagcggc
     2521 acggcaggct gctccttccg cggttccccg cttgcgcgca ccgcaaccgg cgcgaccctc
     2581 cgcaacaccc caaccggctt ccccccgcac gacaccagac aaacgacatc gcggccgcat
     2641 cctcccgtgc gccgccatgt gaccggaccg ccgaacagcc gctccatggt cgcgcggcag
     2701 gcttccagtt ccggttgcgc cgcgcgaaac gctcgactcc gcaaccacca tcgtttcaga
     2761 accctcatcc gccattcccc tatcgttaca catgccgtac cgatctgttt ctgatcatac
     2821 cggatttgca aacacgggac aatcgcaacg tgacgtttca acaacctctt ccgagaacat
     2881 gccttatatg cattaaaaga gcagttgaac cttcaactct ctttggtttc agggtttc
//
LOCUS       DAOSLE010000230         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_29456_length_2929_cov_6.411621, whole genome shotgun sequence.
ACCESSION   DAOSLE010000230 DAOSLE010000000
VERSION     DAOSLE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29456_length_2929_cov_6.411621"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="PK576_10535"
     CDS             complement(<1..1873)
                     /locus_tag="PK576_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOE62062.1"
                     /translation="MRIRHLIVAALWASLLLVLTGPVFAAPMARILRIDPIAATTDGA
                     PVLTLVVDLVQSKRVNAVTGECAGNLDCESERLEKPKALFQPFPYPEGNANFLVTIDG
                     SAFPTKYVWKGRWGENQQMPGVGTAWLILVDAGGSMSSRIGDAKAIANAFVNAMGAND
                     IINIMAFDGKQVVKDSKWLSASRKGEASAFVNGIGANYGSWGRTRPLANIIRAGVNDA
                     FNELGNVGSDIVVPMHQALVVISDGAAGTDPSSTGPGALMLTQHLSKGRFPEDNSALP
                     KTPLPVISIWFPRTQIEELAQNAQEFMMNLANPEFGGFYTNVKAGGAGRAPQIVNAVR
                     DRFNHMHIVKYRVSCIASNVQQTFQLVFTNITPMIGGDATFKDVPVGIDPSTWPLDIN
                     VQYTLDRAKKNPVHPGGKFRVYGDFCWSGDKSRAEVYFLPKNQPAPPSVKGGDIETAK
                     RAQQQLIAMNMRGTAKEASDTFVEFEAPNDEKILLGSGDKAVVRMVIYDNKARRASAA
                     DAQHILTLKGSTAPFPFLWVGGGVFGGLVVLLLLVAIVRAGGSKKQRRAGAAPPAPVV
                     AGYGAPQAQPYAGAAAPVAQPYAGAAQPTAQPYAGTAQPGTQPYAGAAQPAAPAANAG
                     "
     gene            complement(1903..2256)
                     /locus_tag="PK576_10540"
     CDS             complement(1903..2256)
                     /locus_tag="PK576_10540"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE62063.1"
                     /translation="MAETNKAGVGRILRGFLYSFHTKTEGEFWPLFAGQNSIGRADAG
                     EDLDIFIADPTTSSRHATIISEVPGRFVLQDAGSTNGTFVNDQPVGFQGSVEIHDGDR
                     IRLGGYNAAVRFITR"
     gene            2667..>2929
                     /locus_tag="PK576_10545"
     CDS             2667..>2929
                     /locus_tag="PK576_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62064.1"
                     /translation="MLAEAANNWAQLPAVPGADKEQVQAPPAADKEQVQALPGADKEQ
                     VPVRLAAGTAQVPVRLAAGTVQVLVRLAADTAQVPVRPAADTV"
BASE COUNT          577 a          881 c          893 g          578 t
ORIGIN      
        1 aaccggcatt ggcagccggg gccgccggtt gagccgcccc cgcataaggc tgcgttccag
       61 gttgagccgt tcccgcataa ggttgggccg tcggttgagc cgcccccgcg taaggttggg
      121 ctaccggtgc agccgctcca gcgtagggtt gcgcttgcgg tgcgccatat cccgccacca
      181 ccggagctgg cggcgccgcc ccggctcgtc gctgtttttt acttcctccc gctcgcacga
      241 tcgccacgag caacaacaac accacgaggc ctccaaacac tccgcctcct acccatagga
      301 aaggaaacgg cgcggtggag cctttgagcg tgaggatatg ctgcgcatcc gcggcgctcg
      361 ctcgacgcgc cttgttgtcg taaatgacca tgcgtacgac cgctttatca ccgctgccca
      421 gcagaatttt ttcatcgttc ggagcttcga attccacgaa cgtgtccgaa gcctctttgg
      481 ccgtgccgcg catgttcatc gcaatgagct gctgctgcgc gcgttttgcg gtttcgatgt
      541 caccaccttt gacactcggt ggcgcgggtt gatttttcgg caagaaatag acttccgcgc
      601 gggacttgtc cccactccaa cagaagtcac catacacccg aaattttcct cccggatgca
      661 cgggattctt tttcgcccgg tcgagcgtgt actgcacgtt gatgtccaac ggccacgtgc
      721 tcggatcgat gcccaccgga acgtccttga aggtggcatc tcccccaatc atcggcgtga
      781 tgttggtaaa tacgagctga aaagtctgct gcacgttgga ggcaatgcat gacacccggt
      841 acttgacgat atgcatgtgg ttgaaacgat cgcgcacggc attgacgatc tgcggcgccc
      901 gtcccgctcc ccccgccttc acgttcgtat aaaagccgcc aaactccggg ttggcgagat
      961 tcatcatgaa ctcttgcgcg ttttgcgcga gttcttcaat ttgcgtacgc ggaaaccaaa
     1021 tcgaaataac aggaagcggc gttttcggaa gcgcgctgtt gtcctccgga aacctgcctt
     1081 tgctcagatg ctgcgtgagc atcaatgccc caggccccgt cgaagatgga tcggttcccg
     1141 ccgcgccatc ggaaatgacc acgagagcct gatgcatcgg cacgacgatg tcactgccca
     1201 cgttgccaag ctcgttgaag gcgtcgttca cacccgcgcg aatgatgttg gcaagcggac
     1261 gcgttcgtcc ccaactcccg taattggctc caatgccatt gacaaaagca ctcgcctcgc
     1321 ctttgcggct cgcggataac cacttggaat ccttgacgac ttgcttacca tcgaaggcca
     1381 tgatgttgat gatgtcgttg gcacccatgg cattgacgaa cgcattggca atggccttcg
     1441 catcgccaat tcgcgaagac atcgagccgc ccgcatcgac aaggatgagc cacgcggtcc
     1501 caacccccgg catttgctga ttttctcccc aacgtccttt ccatacgtac ttggtaggaa
     1561 aggccgaacc atcgatggtc acgagaaagt tggcgttgcc ttccgggtag ggaaatggct
     1621 gaaagagcgc tttgggtttc tcgagtctct cggactcgca atccagattg cccgcacact
     1681 caccggtgac cgcgttgacg cgtttggatt gcaccaagtc gaccacgagg gtgagaacgg
     1741 gtgcgccatc ggtcgttgcc gctatcggat cgatgcgaag gatacgggcc atgggggccg
     1801 cgaaaaccgg tccggtcaga accagcaaca gagacgccca aagggcagca acgatgagat
     1861 gtcgtattcg catggactgt gttctccgcg gtcatccaga agctatcgtg tgatgaatcg
     1921 aactgccgcg ttgtacccgc cgagacggat tcgatcacca tcgtgaatct cgacggatcc
     1981 ttggaaaccg acaggctggt cgttgacgaa cgttccgttg gtcgaacctg catcttgcag
     2041 aacaaaccga ccgggaacct ccgaaatgat cgtggcatgc cgtgaggatg tggtgggatc
     2101 cgcgatgaaa atatccagat cctcacccgc gtccgcacgt ccaatggagt tctgcccagc
     2161 gaaaagcggc caaaactcac cctcggtttt ggtgtggaag ctatacagaa atcctcgcag
     2221 aatcctgccg acacccgctt tgttggtttc tgccatcgga tcgagcccct gcacacccgc
     2281 ggacggttcc gccggatggg gcggcggact atacatgccc gcggaaggac ctgcgacgcc
     2341 cgggggagcc atggccgggg cctcgacggg cgaagggaaa ggactcatcg cgccggtggg
     2401 aggcaaagga gccggtcccc ccggggaaaa ggcagtggct tcgaatgctt cgcttcccgc
     2461 gatgcgcata tccgccagcg ccggaggtga accatggccc ggcccgtggt tcggtggagt
     2521 gcccgtaaca ccataggggt cggcgggcag cgcggcaccg ggggaaggat atccccctaa
     2581 atccatgggc tggggaggtg aatacggagg catgcctggc accggttggg gtgcataagg
     2641 accaggctcc ggctgtgggt tgaacggtgc tggctgaggc ggcgaataat tgggcgcagc
     2701 tcccggcggt gcctggggcg gataaggagc aggtccaggc accccctgcg gcggataagg
     2761 agcaggtcca ggcactccct ggggcggata aggagcaggt cccggtacgg cttgcggcgg
     2821 gtacggcgca ggtcccggta cggcttgcgg cgggtacggt gcaggtcctg gtacgccttg
     2881 cggcggatac ggcgcaggtc ccggtacgcc ctgcggcgga tacggtgca
//
LOCUS       DAOSLE010000231         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_30403_length_2859_cov_5.278531, whole genome shotgun sequence.
ACCESSION   DAOSLE010000231 DAOSLE010000000
VERSION     DAOSLE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30403_length_2859_cov_5.278531"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..1548)
                     /locus_tag="PK576_10550"
     CDS             complement(394..1548)
                     /locus_tag="PK576_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOE62065.1"
                     /translation="MKIVCDRNMPFAVEAFSLLGEVVLREGRMIGPEDVREAEMLVTR
                     STTRVNAALLDRSAVRFYGSGVIGTDHIDIPYLNARQIVWTGAPGCNAESVANYVTAA
                     LLWLGGRFGFTLAGKTLGVIGVGHVGRRVCAHAKAMGMRVLANDPPRQRDPSDSEARA
                     FVPLDHVLAESDILTCHVPLTREGTDATWHLLNPSTFARLKPGAILINAARGAVIDTG
                     ALLAVLGKRVAQVVIDCWEGEPAYCPELLARTALATPHIAGHSFEGKVNGTAIVYRRA
                     CDFFGIVPAYAFALPEPPVPVWRGDAAGRPDEAVLRDLVLSVYDIEADSDRLRASCVP
                     DAPTRAAAFDRQRGGYPMRREFASTRAALRNASAALCAAVRGLGFAVEKG"
     gene            complement(1552..2586)
                     /locus_tag="PK576_10555"
     CDS             complement(1552..2586)
                     /locus_tag="PK576_10555"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62066.1"
                     /translation="MIKVWGDAEAEAVVALQRGIPLCERPFAEIGRGCGLSEEAVIAL
                     ARRLLASGEARRFGAVFDARRMGYRSVLCAVSVPSEQIVDVAAKVTICPGVTHAYERG
                     WPAELASDLPGGPRGRNWPNLWFTLAAPAGTFAAELETLRAACRPFAIHGLPAIRRFK
                     IDVVFDVRTRDRDERVGPCPPIAEPTSPSVGWDERAREVVRLMQGDLPVDARLFATVA
                     ERLGLDESALLGLLCSWRDAGVLRRVGLLLRHREIGFKANGMCCWDMSEEAVMEAGRR
                     VAAFPEVTHCYERPRMEIFPFRLYAMIHTSDWEETRQLFERISREAGLTDGHLLLSLT
                     EFKKTSMKFF"
BASE COUNT          565 a          991 c          817 g          486 t
ORIGIN      
        1 tttcatccaa atcggggtcg ggatcgctat cgctatcggg atcgatcatt taaccgcgga
       61 acactttctt gggtttcaag gtttctgggg tttctggttt cagggtttca aggtttcagg
      121 gtttcaaggt ttctgggttt catccaaatc ggggtcggga tcgctatcgg ggtcgggatc
      181 gatcatttaa ccgcggaaca ctttcttggg tttcagggtt tctggggttt ctggtttctg
      241 gtttcagggt ttcaaggttt catccaaatc ggggtcggga tcgctatcgg tatcgggatc
      301 ggtatcgtcg acccgcgacc cgcgacattc gccccgcgac gatcgacagg gacgcccgcg
      361 cagcgggaaa tcctgtcaat ttaagcgctg ccgtcacccc ttttcaaccg cgaacccgag
      421 accgcgcacg gcggcgcaca acgccgccga agcgttgcgc aacgccgccc gcgtcgaggc
      481 gaactcgcgc cgcatgggat aaccgccgcg ctgccggtcg aatgcggcag cccgggtcgg
      541 ggcatccggc acgcaggagg cgcgcaacct gtctgaatcg gcctcgatgt cgtacacgct
      601 cagaaccagg tcgcgcaaca ccgcctcgtc cggacgaccc gcagcatcgc cccgccagac
      661 cggcacgggc ggttcaggca gcgcgaatgc ataggcaggc acgattccga aaaagtcgca
      721 ggcgcgacgg taaacgatcg ccgtgccgtt caccttgccc tcaaaggagt gacccgcgat
      781 atgcggtgtc gcaagggccg tgcgcgccaa cagctccgga caatacgcag gctccccttc
      841 ccagcaatcg atcaccacct gcgccacacg ctttcccagg acggccaaca gggccccggt
      901 gtcgatcacc gcgccgcggg ccgcattgat caggatcgcg ccgggcttca gacgcgcaaa
      961 cgtggacgga ttcagaagat gccacgtcgc atccgtccct tcgcgggtca gcggcacatg
     1021 gcaggtgaga atatccgatt cggccagcac atggtcgagc ggcacgaatg cacgcgcttc
     1081 gctgtccgat gggtcacgct gccggggcgg atcattcgcc agcacccgca tgcccatcgc
     1141 cttcgcatgg gcgcagacgc ggcgtccgac atggccgacg ccgatcaccc ccagcgtttt
     1201 acccgccagc gtaaatccaa accgcccgcc cagccagagc agggcagcag tgacataatt
     1261 ggccacactt tccgcgttac atccgggcgc accggtccag acgatctggc gcgcgttcag
     1321 atacggaata tcgatatggt cggtgccgat cacacccgag ccatagaacc gcaccgcgct
     1381 ccggtccagc aatgcggcgt tcactcgcgt cgtcgagcgc gtcaccaaca tctcggcctc
     1441 gcgcacatcc tccggtccga tcatccggcc ttcccgaagg accacctcgc caagaaggct
     1501 gaaggcctcg acggcaaaag gcatgttcct gtcacaaacg attttcatgc gtcaaaagaa
     1561 cttcatactg gttttcttga attccgtcag cgagagcaac aggtgcccgt ccgtcaaccc
     1621 cgcctcccgg gagatccgtt cgaacagttg ccgcgtttcc tcccaatccg aggtgtggat
     1681 catggcgtag agccggaacg ggaagatctc catccgggga cgctcataac agtgtgtcac
     1741 ctccgggaaa gcagcgacgc gccgccctgc ctccatcacc gcttcctcgg acatgtccca
     1801 gcagcacatg ccattggcct tgaacccgat ctcgcggtgg cgcagcaaca atcccacgcg
     1861 gcgcagaacc ccggcatcgc gccaggaaca caacaggccg aggagggcgg attcgtccaa
     1921 gcccaaacgt tcggccaccg ttgcgaacaa ccgcgcgtca accggcagat cgccctgcat
     1981 cagccgcacc acttctcgcg cccgctcatc ccatccgacg gacggggacg tcggttccgc
     2041 aatgggcggg cagggcccga cccgttcatc gcggtcacgc gtgcgcacgt caaagaccac
     2101 atcgatttta aaacgccgga tagccggcaa cccgtgaatc gcaaacggtc ggcaggcggc
     2161 gcgcaacgtc tccaattccg cagcaaaggt tccggcgggc gcggccagcg tgaaccacag
     2221 gtttggccag ttccgccccc gcgggccacc gggcagatcg gaggcaagtt ccgcaggcca
     2281 cccgcgttcg tacgcatgcg tcactcccgg acagatggtg accttcgcag ccacgtccac
     2341 gatttgctca gaaggcaccg acaccgcgca caacacgctg cggtacccca tccgccgggc
     2401 gtcgaacacg gcgccgaacc gccgcgcctc acccgacgcc agcagacgcc gggccaaagc
     2461 gattaccgcc tcctccgaca acccgcatcc tcggccgatc tcggcgaagg gtcgttcgca
     2521 cagcggaatc ccccgctgaa gcgccaccac ggcctccgcc tccgcatccc cccaaacctt
     2581 gatcatcctc gcatcctttt ccgaggggta tcataacaaa aggcggcttc cacgtcgaag
     2641 gcgggtttcc ccgcatcaaa ccgtgagggt cccgaagacc ggaacggttc gagcaaacgc
     2701 ctgagtgccc tttacgtcgc gtaatccggg attgcgtcgc gcatgaaacc ccgcagcgct
     2761 tctcaccagg taagccaccc gtcttccggg gtacggtttt cggcgcgcag ccacatcata
     2821 agcgccacgc ggaaatcgtc atcccgctcg ctgatccgg
//
LOCUS       DAOSLE010000232         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_32867_length_2692_cov_8.417899, whole genome shotgun sequence.
ACCESSION   DAOSLE010000232 DAOSLE010000000
VERSION     DAOSLE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32867_length_2692_cov_8.417899"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..944
                     /locus_tag="PK576_10560"
     CDS             174..944
                     /locus_tag="PK576_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62067.1"
                     /translation="MERTTQNEPVNILCLKWGSRYPAYYANALYQGFRRFLHRPFRFV
                     CVTEDTQGLDQGIETFPIPENPGMSIAWPNVFLKLLVTADGFAGLQGPTLFCDLDVVL
                     TGELDCFFDFHPGKPCIIHNWIETRKLLFRPRPAIGNSSVFRFEAGKSQYICDTFLRE
                     RDRAVDPAHFRTEQAFLTYAMRECHWWPETWVRSFKRHSLRPFPLNLCSTPRLPRDTR
                     ILVFHGYPDPDEALRGYRGKRPHQHALPAPWIADYWHT"
     gene            complement(941..1597)
                     /locus_tag="PK576_10565"
     CDS             complement(941..1597)
                     /locus_tag="PK576_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62068.1"
                     /translation="MKTRQMLDEGQWIEIGHGSRRKCFRVGNEPVCVKFYRLPSEYTH
                     KTTWGVRLHIGLARFRPHFNVNCQEWRYHRALRERLPPDLFAAFPEMLEPLFSPSRGW
                     GVVESLIENADGSPIRRVIAEMARTEDEGLRRRLYAAAEGLFTQLAEQAVCFYDPPNV
                     MVQWTGPETFRLRIVDFEPRGRALVPGLSRIRPYVRAKVRRRCARYLKRLRETFLQTG
                     "
     gene            complement(1723..2670)
                     /locus_tag="PK576_10570"
     CDS             complement(1723..2670)
                     /locus_tag="PK576_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOE62069.1"
                     /translation="MGWRGKTLGGSIGSFFGPWGALAGAAAGHFLVDRKKTRRAVNSP
                     LRLLAVTAAALREVAEADGRYTLREDRVIRAVLDGIRGEIGAPLGPHELAYLIDDAAR
                     IDQGLLRLAALARDMPGLGHAAVVWLWRVAVSEAGETSDGAARIARFAQQAGLSSGEA
                     RFCALPYSRTACADPSGVARRADCDTLGVPYDADPVQIKAAYRALSLTYHPDKHAGLD
                     PAIRALTAAKFTEIKAAYDRLRGGSRAGLLVRLAGADRLVPYADGVTAACFGCGRSVR
                     LHEESDPLTLRCDACQMLFAMEPERLAAMENSRGASPFS"
BASE COUNT          526 a          940 c          746 g          480 t
ORIGIN      
        1 cctgtcacga accgattccc ctgaaaaggc aacaacgaga ttctcaaagt aaatgcgacc
       61 gccccagtcg catttacttt gccaagtgag gctgtactgt aatcctgctt tcgctctctt
      121 gacacggggc ttcgtctcaa atattctgtt tcatacaagg aggagtatca ttcatggaac
      181 gaaccacaca aaacgaaccc gtgaacatcc tttgtttaaa atgggggtca cgctatccgg
      241 cgtattatgc aaacgccttg tatcagggtt tccggcgttt tctgcaccgc cccttccgat
      301 tcgtatgcgt gactgaggac acgcagggtc tcgaccaagg gatcgagacc ttcccgattc
      361 ccgaaaaccc aggcatgagc atcgcgtggc cgaatgtgtt tttgaaacta ctggtgacgg
      421 ccgatggctt tgccggattg cagggcccca cgcttttttg cgatttggat gtcgtgctca
      481 ccggcgagct ggactgcttc ttcgactttc atccgggcaa accctgcatc attcacaact
      541 ggatcgaaac gcgtaagctg cttttccggc cacgaccggc catcggcaac tcgtcggtct
      601 ttcggttcga ggcgggaaag tcgcaatata tctgcgacac ctttctccgc gaacgggatc
      661 gggccgtcga ccccgcgcac ttccgcaccg aacaggcgtt tctcacttac gcgatgcgcg
      721 aatgtcactg gtggccggaa acctgggtga ggagcttcaa gcggcacagc ctgcggccct
      781 tccccctgaa cctgtgttcc accccgcgat tgccgcgtga cacccggatt ctggttttcc
      841 acggctatcc ggatccggac gaggccctgc gcgggtatcg cggcaaacgt ccccatcagc
      901 atgccctgcc cgccccctgg atcgcagatt attggcacac ctaacccgtc tgcagaaacg
      961 tctcccgtaa ccgcttgaga tatcgcgcgc accgccgccg cactttggcg cggacatagg
     1021 gccggatccg cgagaggccg ggcaccaagg ccctccctcg cggctcaaag tccacaatgc
     1081 gcaggcggaa cgtctccggc ccggtccact gcaccatcac gttgggcggg tcgtaaaaac
     1141 agacggcctg ttccgccaac tgcgtgaaca acccctcggc cgccgcatac agacgccgcc
     1201 gcaacccttc gtcttccgtc cgcgccatct ccgcgatcac ccggcggata ggcgaaccgt
     1261 ccgcattctc gatcaggctc tcgaccaccc cccagccacg cgacggcgag aagagcggtt
     1321 cgagcatttc cggaaaggcc gcgaacaggt ccggcggcag acgttcgcgt aaggcgcggt
     1381 gataacgcca ctcctgacag ttgacgttga aatgcggccg gaaccgcgcg agcccgatgt
     1441 gcagccgtac cccccaagtg gtcttgtgcg tgtactccga gggcaatcgg taaaatttca
     1501 cgcagaccgg ttcgtttccg acccggaaac atttgcgtcg gctcccgtgc ccgatctcaa
     1561 tccactggcc ctcgtccaac atctgcctcg tcttcatcta ctcctcaatc accatccgga
     1621 acccggcacc ggaagaccgt acccgccaca ccggaccgta accggaactg cattatgagg
     1681 ggagcacgga aagccgtcaa ccccgtgccc atattgtcat gatcaagaaa acggggatgc
     1741 gccccgactg ttctccatcg cggccagccg ttcaggctcc attgcgaaca gcatctggca
     1801 ggcgtcacac cgcagcgtca gcggatcgga ctcctcgtgc aggcggacac tccggccgca
     1861 tccgaagcaa gccgccgtca cgccgtccgc gtacggaacc agccggtcgg cccccgccag
     1921 ccgcaccaac aacccggcgc ggctccctcc gcggagtcgg tcgtaggccg ccttgatctc
     1981 agtgaatttc gcggcggtca gcgcccggat cgcaggatcg agacccgcat gtttatcggg
     2041 atgataggtc agactcagcg cgcgatacgc ggctttgatc tgcacggggt cggcatcata
     2101 cggcacaccg agcgtgtcgc agtccgcgcg ccgggcaacg ccggaggggt cggcgcaggc
     2161 cgtgcggctg tacggcaacg cgcagaaccg cgcctccccg gacgaaaggc cggcctgttg
     2221 cgcgaagcgc gcgatccggg cggccccgtc cgacgtctcg cccgcttcgc tgaccgccac
     2281 gcgccacagc cagaccacgg cggcatgccc gagtccgggc atgtcccgcg ccaaggccgc
     2341 caaccggaga agcccctgat cgatacgcgc ggcatcgtca atcagatacg ccaactcgtg
     2401 cgggccgagc ggcgcgccga tttcgcctcg gattccgtcg agaaccgcac ggatcacccg
     2461 gtcttcccgc aacgtatacc ggccatccgc ttcggccacc tcgcgcaatg ccgcagccgt
     2521 gaccgccagc aggcgcagcg gcgaattgac cgcacggcgc gtctttttgc ggtcgaccag
     2581 aaaatgaccc gccgcagccc cggccagtgc cccccacgga ccgaaaaacg agccgatgct
     2641 gcctcccaac gttttgcccc gccaccccat acgcccttac gttatcgtaa aa
//
LOCUS       DAOSLE010000233         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_37182_length_2448_cov_7.078562, whole genome shotgun sequence.
ACCESSION   DAOSLE010000233 DAOSLE010000000
VERSION     DAOSLE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37182_length_2448_cov_7.078562"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..109)
                     /locus_tag="PK576_10575"
     CDS             complement(<1..109)
                     /locus_tag="PK576_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOE62070.1"
                     /translation="MAKVLGIDLGTTNSCMSVMEGGEPVVVPNAEGMRTT"
     assembly_gap    862..961
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <962..2325
                     /locus_tag="PK576_10580"
     CDS             <962..2325
                     /locus_tag="PK576_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62071.1"
                     /translation="STPPQRPAPSANRDPGSRIFSGTRADRTEPRKPSRPAPSVSTPP
                     QRPAPSANRDPGSRIFSGTRTAPSQRSDQRSESPSPASVRSVPQTPSVSRPDSRTRSD
                     TPRRITLDKRNGIRRGNDRPLRGESRTHLPERFASIQRNDRASPLSLRAPDGLSDSRT
                     PRLTPRRESVRGRPESRLRNVRPTPFRAPPRVYRPSPHRHPTARYRHRSPYVFDPWTF
                     RHHHRGFSHIVYRPVPIYAASGMSFFWSSGAFALGFSTYSWPSYGYTYTRYYDSWHDG
                     YWGYSNLYLGGWRNGWYGGFSYIYNPWPVYRTYYFYEPAPVVTRVETVYVEQAAPATV
                     TATTTSSATVAPPATSAVEEPVGAWAEAPAPERVEAQSYRCFCACGCNGTRPCFCDYP
                     CGAEYEDITTRTDLRLGYVSYAESLNPEAIWASYAGLDYATDEVYDQPDTETAAYSDR
                     DLL"
BASE COUNT          438 a          800 c          626 g          484 t
ORIGIN      
        1 gcgtcgtgcg catcccttcg gcgttcggaa ccacgaccgg ctcgccgcct tccattacgg
       61 acatgcagga gttggtcgtc cctaagtcaa ttcccaaaac ttttgccatt ttacgctccg
      121 tttcatccgc tctaactgaa caccctatac gcggatacgg ggtataatgc accttgtgtg
      181 ccaaaatcct ttcgacatcc ggactgtttt atttataacc tgtttttttg caagtggtta
      241 gaaaaaatat ccttccttaa tttatttggc ctgaagggtg cggtttcttg ttttggcaca
      301 cttgtcatgg cacagttttc acaaagtggg tcataattgc tcgccgcgtc acggcttctt
      361 tcttgccgtt ttaatcggat gtttaaaata tggccgatcg aaggcaatac gaagaggcat
      421 aaggcgttta tctttacgtg tggggaggtg caagtggtct tgacgggccc gccccgagcc
      481 cctaaactgc ggattatgag cctttgaccg aaaatggctg gatgggaaga aaggagcgca
      541 ccatgaaaac aacaagatgg ctcattgttt tgatggccgt aacggggttc gggttggcgt
      601 catcggctcg ggagcgctcc tcgtctagca gccggccgcg tggcgcgcct gcctcttcca
      661 aacaaagcgc atcctcctat caaacaggac gtaacgcttc atcatcctca cgcccctcag
      721 caccgcgctc ccaaatggcg gctcaaccgc ggtcagcgca tgtgccgtcg ccgaaaaccc
      781 cgtaccgcat gccggcaccc cgcgtctcac aaccgttctc ctcatccgtc tcccggcagc
      841 agtcccggcc cgcgccctcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 ntctccacac ccccgcaacg gcccgcgccc tccgccaacc gcgatcccgg ttcgcgcatc
     1021 ttcagcggca cccgcgccga tcggacggaa ccccgaaaac cgtcccggcc cgcgccctcc
     1081 gtctccacac ccccgcaacg gcccgcgccc tccgccaacc gcgatcccgg ttcgcgtatc
     1141 ttcagcggca cccgcacggc cccctcacag cgttcggatc aacggtccga atccccatcc
     1201 ccggcatccg tccgttcggt gccgcaaact ccaagcgttt cccggccgga ttcccggaca
     1261 cgttcggaca cgccccgccg gatcacgctg gataaaagga acggaatccg ccgcgggaat
     1321 gaccgtccgt tgcgcggcga atcgcgcacg caccttccgg aacgctttgc ttccatccag
     1381 cgtaatgatc gcgcctcccc gctcagcctt cgcgcgcccg acggactctc cgattcccgc
     1441 actccccgct tgacgccccg gagagaaagc gtacggggcc gtccggaaag ccgactccgc
     1501 aatgtgcggc ccacaccgtt ccgcgcgcca ccccgtgtgt accgtccttc cccgcaccgg
     1561 catccgacgg cgcgataccg ccaccgctcg ccgtatgtgt ttgatccgtg gacgttccgg
     1621 catcaccatc gcgggttctc gcacatcgtc taccggccgg tgccgattta cgcggccagc
     1681 ggaatgagtt tcttctggtc aagcggcgcg ttcgcactcg gcttcagcac ctactcgtgg
     1741 ccgagttacg gttacacgta cacgcggtat tacgacagtt ggcacgacgg atattgggga
     1801 tactccaacc tctatctggg cggatggcgc aacggttggt acggcgggtt ctcctatatc
     1861 tacaatccgt ggccggttta ccggacctac tacttctatg aacccgcccc agtggtgacg
     1921 cgggtggaga ccgtgtatgt cgaacaggcg gccccggcca ccgtgaccgc aaccacgacc
     1981 tcatccgcaa cggtcgcccc tccggccacc tcggcggtgg aagagccggt cggcgcatgg
     2041 gcggaagccc ccgcgccaga acgggtcgag gcgcaatcgt accgctgttt ctgcgcttgc
     2101 ggctgcaacg ggacaaggcc ctgcttctgt gactatccgt gcggtgcgga atatgaggac
     2161 atcacgaccc ggaccgatct ccgcctcgga tatgtctcgt atgcggaatc cttgaatccg
     2221 gaagcgatct gggcttcgta tgcgggattg gattacgcca ccgatgaggt gtatgaccaa
     2281 ccggacacgg aaaccgcagc ctactcggac cgcgacctgc tctgacatgc aacccgatgg
     2341 tctttccgtt ccatcttttc cgtacgcagc tcgcccgcat ccggaatcag aattagcctg
     2401 ccgcgcggct tcgcttcggc gggaagaaat ccgtgattaa catgatgt
//
LOCUS       DAOSLE010000234         2424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_37669_length_2424_cov_9.495990, whole genome shotgun sequence.
ACCESSION   DAOSLE010000234 DAOSLE010000000
VERSION     DAOSLE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37669_length_2424_cov_9.495990"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2268)
                     /locus_tag="PK576_10585"
     CDS             complement(61..2268)
                     /locus_tag="PK576_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOE62072.1"
                     /translation="MLCRMSRLGVLGVVLLGSRGIFASERQTLSLDGEWEIADSLLPE
                     VIPAAFPHTVPVPGLANLAKPAFPDVDRFISRENLSRRILHKLVPDDWLEKYWVGKVE
                     QDRNYFWCRRTFRAPKERAAALLKINKAQFGTAVWLNGLKVGESPGCFTASVFALGKA
                     IRWNAENTLVVRIGAHPAVLPDTFPTGSDFEKNTWSPGIYDSVSVSFCDNPVIESIQI
                     APRIASGEALVQTRVKNHSDAPVNASVFHTVKTWKRAKPVAMGEAVAVTLPPGGERVL
                     TQTIQIPDARLWSPEDPFLYVVETHTGGDSVATRFGMREFRFDAATGRAYLNGRVYFL
                     RGSNITLHRFFEDPLCKALPWNEKWVRKLLGEVPKKMHWNYFRFCIGPVPDRWFDICD
                     EVGLLIQNEFFVWTGAPGWDKRYSRTHDNAEMIRQYTDWMRDHWNHPSVVVWDANNET
                     MDASFGDIIIPAVRPLDLSNRPWENSYNKPVAPDDPVEDHPYLMIGGRHKPLKFKMSD
                     LETMDGKPLDKLPSDTNPKIINEYGWLWLDRDGTPTLLTENVYAQLCGTNSTARERLD
                     LYAYLLAGKTEFWRAHRQYAGIIHFVYLTCGYPGVYTADHFADVTRLKLDPAFADYMS
                     EAFKPLGVYVNFFQPTLKAGETRVFKVMLVNDESIPLSGQITLELIRRGKSSVLVRAE
                     QPFQIKPAGTASVAIALDMPDTEGECVLKAVAAADQKKDVSPTVCRRWVRLAR"
BASE COUNT          465 a          763 c          728 g          468 t
ORIGIN      
        1 ggcactggcc gcaggcggcg gaacaggacg cattcagcgg taagagggcg tgatatgggg
       61 ctaccgcgcc agccgcaccc aacggcggca aaccgtcggg ctcacgtctt ttttctggtc
      121 tgcggcggca accgctttca gtacgcactc cccctcggta tccggcatat ccaaggcgat
      181 cgcaaccgaa gccgtaccgg ctggcttaat ctgaaacggc tgttccgcac ggaccagaac
      241 gctgctttta ccgcggcgga tcagttcaag cgtgatctgt ccgctcagcg gaatcgactc
      301 gtcgttgacc agcatgactt tgaaaacacg cgtctcgccc gccttcagcg tgggctggaa
      361 gaaattcacg tagacgccca acggtttgaa tgcctcgctc atgtagtcag cgaaggccgg
      421 gtccaacttg agccgggtca catccgcgaa gtggtccgcc gtatagacac ccgggtaacc
      481 gcacgtcagg tagacgaagt ggataatgcc ggcgtactgg cggtgcgcgc gccagaactc
      541 ggtcttgccc gccagcagat atgcgtagag gtcgagccgt tcgcgggcgg tgctgttcgt
      601 cccgcagagc tgcgcgtaca cgttctccgt gaggagtgtc ggcgtgccgt cgcggtcgag
      661 ccagagccag ccgtattcgt tgatgatctt cgggttcgtg tccgaaggca gcttgtccag
      721 cggtttgccg tccatcgtct ccaggtcgct catcttgaac ttcagcggtt tgtgccggcc
      781 gccgatcatg agatacgggt gatcttccac gggatcgtcc ggcgcaaccg gcttgttgta
      841 gctgttctcc cagggacggt ttgacaggtc gagcggacgc acggccggaa tgatgatgtc
      901 gccgaaactt gcgtccatgg tctcgttgtt cgcgtcccag accacgacgc tgggatggtt
      961 ccagtggtcg cgcatccagt ccgtgtactg ccggatcatc tcggcgttgt catgcgtgcg
     1021 cgagtacctt ttgtcccagc ccggcgcgcc ggtccagacg aaaaactcgt tctggatcag
     1081 caggccgacc tcgtcgcaga tgtcgaacca gcggtcaggc accggaccga tgcagaaccg
     1141 gaaataattc cagtgcatct tcttcgggac ttcaccgagg agtttgcgca cccacttctc
     1201 attccacggc agcgccttgc acaacgggtc ttcgaagaac cgatgcaggg tgatgttcga
     1261 gccgcgcaga aagtagaccc tgccgttcag atatgcgcgt ccggtggcgg cgtcaaaacg
     1321 gaactcgcgc atgccgaagc gcgtcgcgac cgaatcgccg cccgtgtgcg tctcgacgac
     1381 gtacaggaac ggatcctccg gcgaccagag acgcgcgtcg ggaatttgga ttgtctgcgt
     1441 cagcacccgt tccccgccgg gcggcagcgt gacggcgacc gcttcaccca ttgcgaccgg
     1501 tttcgcccgc ttccaggtct tcacggtgtg aaacacggat gcgttgaccg gcgcgtcgga
     1561 gtggtttttc acgcgggtct gaaccagcgc ctcacccgag gcgatgcgcg gcgcgatctg
     1621 gatggattcg atcacggggt tgtcgcagaa actcaccgac acgctgtcgt agatgcccgg
     1681 cgaccatgtg ttcttttcga aatccgaacc ggtcgggaag gtgtccggca acacggccgg
     1741 atgcgcgcca atgcggacca caagcgtgtt ttcggcattc cagcggatcg ccttgccgag
     1801 cgcgaaaaca ctggccgtga aacaaccggg ggactcgccc accttcaacc cgttcagcca
     1861 gacggcggtg ccgaactgcg ccttgttgat cttgagcagg gccgcggcgc gttccttcgg
     1921 cgcgcggaag gtccgtcggc accaaaaata gttgcggtcc tgctccacct tgcccaccca
     1981 gtacttttcc agccaatcgt cagggacaag cttatggaga atacggcggg acaggttttc
     2041 gcggctgatg aaccggtcca catccgggaa ggccggtttg gccaaattgg ccaaaccggg
     2101 cacaggcacc gtgtgcggga acgccgcggg gatcacctcc ggtagcaggc tgtcagcgat
     2161 ctcccattca ccatccagcg acaacgtctg ccgctcagac gcgaaaatcc cgcgagaccc
     2221 caacaataca acgcccaaca cgccgagacg cgacatccga cacagcacgt ttttatcctt
     2281 agttaagcct tatcaccgtt cctttgaccg gtatcatatg taaaaactcg tccggcgtca
     2341 acacgcggtc actgagccgc acctcgtcga tccagccgtc gaacgagcgc gtgcggcccc
     2401 cgctggaccc tccaaaccgg atgt
//
LOCUS       DAOSLE010000235         2390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_38372_length_2390_cov_7.355460, whole genome shotgun sequence.
ACCESSION   DAOSLE010000235 DAOSLE010000000
VERSION     DAOSLE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38372_length_2390_cov_7.355460"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..722)
                     /locus_tag="PK576_10590"
     CDS             complement(201..722)
                     /locus_tag="PK576_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62073.1"
                     /translation="MKYVFNPRNRQINPVTEQTFVDATLRDKAVSPELYALVCKGKIS
                     VEDVAALFAVNKSPEILVKNHKAVKESPVATAPANTATVPEDTTPPPPAPGSTADEND
                     GKATADAAFDGRELCAKKTEELSILAATIGCPEMKDPAFAPTRGNLIKAIMAKAKAAE
                     GDEKAPGAPKAAE"
     gene            complement(793..1392)
                     /locus_tag="PK576_10595"
     CDS             complement(793..1392)
                     /locus_tag="PK576_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62074.1"
                     /translation="MATTYTAPNKLVGFADSVNATGRFGRSAFALFVDFAKVGAPAAT
                     GLVVSDIVDGIGALPKNCVVRTVAAQIVKPITVLSEDGISALPITLKAGSTTLASMTL
                     ADVNGPALGAEWTDVLLSNGSTGVAGAKENLSLTIGAVLDDADTPVATAITGGVLMLV
                     FFVDYIPEVDELNALFNTVNEISDGGNTATEITDFGLGY"
     gene            complement(1426..>2390)
                     /locus_tag="PK576_10600"
     CDS             complement(1426..>2390)
                     /locus_tag="PK576_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62075.1"
                     /translation="GYIFTWEAYVRSLPMIQGRDLITQFPESKDVEWIPQRAQYTQIG
                     TTRKQRRESRWDLTGEFLASGMQDFAKNRFKEYLDFCIQNAGTYNKGATVAVSGGPDL
                     GYGGRLLGKVLLGTPTLPVILFKDQAALVASSATGPKMLAVDFVASLDVALSEQIGGD
                     GGDTVMAGATLFDQQRSTESFVYYPALLNTYCKLSDKAISATAQDERRNSLFGDVMWL
                     NSTHLGGWNRAIKTNLLPTFVTDGTGGASFAPAGVKVIPIIFGQNRSVMATPPYTDSD
                     RQIKDQKNLLDVDRDVRYYDFFAAFPNFLGVAYVTFDEYAAQTA"
BASE COUNT          449 a          752 c          716 g          473 t
ORIGIN      
        1 gcggcgcatc tctttccaga cgcccgtgcg ccgaagaaat tcccgcgccg cctcacagac
       61 cgccttgcgt atctccagct cattcttgcc gggggcgcgg tatacgacat cctcggtgac
      121 ggctttcaag tccgtcacct gtaccggttc cagtgtgtaa ccgcccgttg tcatatcgct
      181 accctttcat cgtgtgccgc ttactccgcc gccttgggtg cgccgggggc cttctcatcg
      241 ccctccgccg ccttggcctt cgccatgatc gccttgatca gattaccgcg cgtgggggca
      301 aatgccggat ctttcatttc cgggcagcca atggtggccg caaggatcga aagctcctcg
      361 gtcttcttgg cgcacaactc gcgcccgtca aacgccgcat ccgcagtggc cttgccgtcg
      421 ttctcatccg ccgtgctgcc gggggccggg ggcggtgggg tcgtatcttc cggaaccgtc
      481 gcggtgttcg cgggggcggt ggcgaccggg ctctctttga ccgccttatg gttcttcacg
      541 aggatttcgg gggacttgtt caccgcaaag agggcggcta catcctccac gctgatttta
      601 cctttgcaga ccagcgcgta aagctcggga gaaacggcct tgtcccgcag ggtggcatcg
      661 acgaatgtct gctccgtgac ggggttgatc tggcggtttc gagggttgaa gacgtatttc
      721 atagatagcc tttcggtgga atacccccgg aggtgtcacc ctccggggat gattgaatcg
      781 atgcttcttc ggttagtagc ccaggccgaa gtcggtgatc tccgtggcgg tattgccgcc
      841 gtcggaaatt tcattcaccg tgttgaacag cgcgttcaac tcatccactt cggggatgta
      901 atccacgaag aagaccagca tcaacacgcc gcccgtgatt gccgtggcaa ccggcgtgtc
      961 cgcatcgtcc agcaccgcgc cgatagtcag gctgaggttt tccttggccc cggcaacgcc
     1021 ggtcgagcca ttggaaagca acacatccgt ccactccgcg ccaagcgccg ggccgttcac
     1081 atccgccagc gtcatggacg caagcgtggt ggaacccgcc ttgagcgtga tcggcagtgc
     1141 ggaaatgcca tcttcagaca gaaccgtaat cggtttgacg atctgcgccg ccaccgtgcg
     1201 aacaacacag tttttcggca acgcgccgat gccgtcaacg atgtccgaga ccaccagacc
     1261 ggtagcggcc ggtgcaccca ctttggcgaa atcgacaaag agggcaaacg ccgaacggcc
     1321 gaaacggccc gtggcgttaa cgctgtcggc aaagccgacc agcttattgg gagcagtgta
     1381 ggttgtagcc atgaaagggg cctccttcag acccattgtg atcggttagg ccgtctgcgc
     1441 ggcatactcg tcgaacgtga catacgccac gcccaagaag ttcgggaaag ccgcgaagaa
     1501 atcgtagtac cgcacatcgc ggtccacgtc cagcagattc ttctgatctt tgatctgccg
     1561 gtcgctgtcg gtgtagggcg gcgtcgccat gaccgagcgg ttctgaccga agatgatcgg
     1621 aataaccttc acacccgccg gggcaaagct cgcgccgccc gtgccgtccg tgacaaacgt
     1681 cgggagcagg ttggtcttga tcgcccggtt ccagccgccg aggtgcgtgg agttgagcca
     1741 catcacatcg ccgaacagcg aattgcgcct ctcgtcctgg gcggtcgcac tgatcgcctt
     1801 gtcggagagc ttgcaatagg tgttcaggag ggcagggtag tacacgaacg actccgtgct
     1861 gcgctgctgg tcgaacaggg tggcaccggc cataaccgtg tcgccgccgt ccccgccgat
     1921 ctgttcggac agagcgacgt ccagagacgc cacaaagtcc acagccagca tcttcgggcc
     1981 ggtcgcgctg gacgccacca acgcagcctg atccttgaac agaatcacag gcagagtcgg
     2041 ggtgccgagc agaaccttac cgagcagacg cccgccgtac ccaagatcag gcccgccgct
     2101 gacggcaacg gtcgcgccct tgttgtaggt gccagcgttc tggatgcaga agtccagata
     2161 ctccttgaac cggttcttgg cgaagtcctg catgcccgag gccagaaact ccccggtcaa
     2221 atcccaacgg gactcacgcc gctgcttgcg ggtggtgccg atctgggtgt actgcgcacg
     2281 ctggggaatc cactccacgt ccttcgactc ggggaactgc gtgatcaagt cacggccctg
     2341 aatcatcggg agcgagcgga catacgcctc ccacgtgaag atgtagccga
//
LOCUS       DAOSLE010000236         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_39201_length_2351_cov_5.662021, whole genome shotgun sequence.
ACCESSION   DAOSLE010000236 DAOSLE010000000
VERSION     DAOSLE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39201_length_2351_cov_5.662021"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..638
                     /gene="gatC"
                     /locus_tag="PK576_10605"
     CDS             342..638
                     /gene="gatC"
                     /locus_tag="PK576_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOE62076.1"
                     /translation="MKDTINVAYVAELARIELTPEEQQLFQRQLETVVRYVEKIGRLE
                     LSGIEPTQHGQNLVNVFREDQTVAAESEARELFLANAPERNGYEFRLPKIVEEA"
     gene            694..2130
                     /gene="gatA"
                     /locus_tag="PK576_10610"
     CDS             694..2130
                     /gene="gatA"
                     /locus_tag="PK576_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOE62077.1"
                     /translation="MDVAGLTLCAAAEGLRKKTYSSVELTRAVLDAIREKDGALHAYL
                     SVDEEGALRQAAAADAARAAGHDTDLLGIPVAIKDLINVEGQPCTCASNMMQGYVAPY
                     DATVAARLRAAGAVFAGRTNMDEFAMGSSSETSAFKVTRNPHDLERVPGGSSGGSAAA
                     VAGGEAIAALGSDTGGSIRQPAAFCGCVGLKPSYGRVSRYGVTAYASSLDQVGPMTRT
                     VEDAAVLLQTLAGVDPHDGTTLDTPVPDYRAALVGGVRGLRIGLPKEYFVDGTDPEVM
                     ACVRRAVDACATAGAEIVEVSLPHTEYAVAVYCIVGTAEASANLARFDGVRYGHRTSR
                     PASVFDLYARSRAEGFGQEVKRRIILGTYVLSSGYYDAYYGRAQKVRTLIRRDFTEAF
                     ERCDVLFTPVAPTPAYRIGERCEDPLTMYLGDTFTVPASLAGICGLSVPCGRTAAGLP
                     VGLQILGPAFGESVVLRAGQAVEQEVRA"
     gene            2127..>2351
                     /locus_tag="PK576_10615"
     CDS             2127..>2351
                     /locus_tag="PK576_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE62078.1"
                     /translation="MSRYLVTIGLETHVQLKTLTKMFCGCSLTFGEEPNTNVCPVCLG
                     YPGALPVMNREAVRLTVLSGLMLGCEINRHS"
BASE COUNT          417 a          613 c          884 g          437 t
ORIGIN      
        1 aagccaggat tacaggatta acaggatttc tgattgaggt tatcggtagg ccggccgtcc
       61 ggggttccgg gcgagaacag ggttgtgacg ggaacgctta acagtatgca gtgaagaggg
      121 gagagggagg tgcgcgggcg gatgcccgct ggcgggtgtt aaggctgtgg ggctgtaagg
      181 gaggggcaag cagacgcttg cccctcccga tctgagacat cgctatcgga tcgatacgtt
      241 caaacgtatt tgaattgtac cttcaggctg gaaaaggtaa aatagaactc tttcttttta
      301 caggtgccgg aggcggacgg aagctcagga aggaaccgca ggtgaaggac acgattaatg
      361 tggcgtatgt ggccgaattg gcgcggatcg aactgacgcc ggaggagcaa cagttgtttc
      421 agcggcagtt ggagacggtt gtgcgttacg tcgaaaagat cggaaggttg gagctctccg
      481 gcatcgagcc gacgcagcac ggccaaaatc tggtaaatgt gttccgtgag gaccagacgg
      541 tcgcggcgga gtcggaggcg cgcgagctgt ttctggcgaa cgcgccggaa cgcaacgggt
      601 atgagtttcg gttgccgaag attgtggagg aagcctgaac gggagtgtgc gcgggcagcg
      661 gaacgggcag ggacgaaggc gggagtcgac acgatggatg ttgcgggttt gacactgtgc
      721 gcggcggcag aagggctgcg gaagaagacg tattcctcag tcgagctgac acgggccgta
      781 ttggatgcga tccgggagaa ggatggcgct ctgcacgcgt atctgagtgt ggatgaggag
      841 ggggcgttgc ggcaggccgc cgcggcggat gccgcgcggg cggcggggca cgacacggac
      901 ctgctcggga ttccggtggc gatcaaggac ctgatcaatg tcgagggcca gccctgcaca
      961 tgcgcctcca atatgatgca ggggtatgtg gctccgtacg acgcgacggt tgcggcgcgg
     1021 ctgcgggccg caggcgcggt gtttgcaggc cgcaccaata tggacgaatt cgccatgggg
     1081 tcgtcttctg agacctcggc gttcaaggtc acgcgcaacc cgcacgatct ggaacgggtt
     1141 ccgggcggct ccagcggcgg atcggctgcg gcggtggccg gcggcgaggc cattgcggcg
     1201 ttgggtagcg atacgggcgg atcaatccgc cagcccgccg cattctgcgg ttgcgtgggc
     1261 ctcaaaccct cgtacggccg cgtgtcgcgg tacggcgtca cggcgtacgc ctcatccctg
     1321 gatcaggtgg ggcccatgac ccggacggtt gaggatgcgg cggttctgtt gcagacgctg
     1381 gcgggtgtgg atccgcatga cgggaccacg ttggacacgc cggtgccgga ttaccgggcg
     1441 gcactcgtcg gcggcgtgcg ggggttgcgg atcggcctgc cgaaggagta ttttgtcgat
     1501 ggcacggatc cagaggtgat ggcgtgtgtg cgccgggctg ttgatgcgtg cgcgacggcg
     1561 ggggccgaga ttgtggaggt gagcctgccg cacaccgaat atgcggtcgc ggtgtattgc
     1621 attgtgggca cggcggaggc gtccgccaat ctggcgcgtt ttgacggggt gcgctacggg
     1681 caccggacga gccgccccgc atcggtgttc gacctgtacg cccgtagccg ggccgagggg
     1741 ttcggccagg aggtcaaacg gcgcatcatt ctggggacgt atgtcttgag cagcgggtat
     1801 tacgatgcgt attacgggcg cgcgcagaaa gtccggaccc tgatccggcg cgactttaca
     1861 gaggcgttcg agcgttgcga tgtgctcttc acgccggtgg cgcccacgcc ggcgtaccgc
     1921 atcggcgagc ggtgtgagga tcccctcacc atgtatctgg gcgacacgtt cacggtgccc
     1981 gccagtctgg ccgggatctg cggactctcc gtgccgtgcg gccggactgc ggcaggatta
     2041 ccggtcggac tccagattct cggtccggcg ttcggtgaga gcgtggtgct gcgggcaggg
     2101 caggcggtcg aacaggaggt gcgggcatga gtcgttacct cgtcaccatc ggactggaaa
     2161 cgcatgtgca gctcaagacc ctcaccaaga tgttctgcgg ttgcagtctg acgttcggcg
     2221 aggagccgaa caccaatgtc tgcccggttt gcctcgggta tccgggcgcg ttgccggtga
     2281 tgaaccgcga ggccgtccgg ttaacggttc tctccggcct gatgctggga tgcgaaatta
     2341 accggcatag c
//
LOCUS       DAOSLE010000237         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_39926_length_2319_cov_8.694788, whole genome shotgun sequence.
ACCESSION   DAOSLE010000237 DAOSLE010000000
VERSION     DAOSLE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39926_length_2319_cov_8.694788"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PK576_10620"
     CDS             <1..611
                     /locus_tag="PK576_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62079.1"
                     /translation="LTVRGNPTFADVVSFFSSAGIDIKAGNIDGSYEMFENQPPQAIV
                     TAVLITAINALKEHARKNGAVYASGAEIPDAPPGGVAYCTGGSKGWSGKGKGCFIFEG
                     DVSFQGSGIDIESVNNYPALIVLSASEVKFNADAVIHGALLFPNASFTVNGHAEIHGV
                     ILVGQSARINGTADLYPGEGGRGFEVPPEERVKDNVVITAWH"
     gene            648..1751
                     /locus_tag="PK576_10625"
     CDS             648..1751
                     /locus_tag="PK576_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62080.1"
                     /translation="MAYAKIPEKEVSLLVRALGLLVNQAGVYGPTHNVTQRAARSVFA
                     ELDEIVGRYGAVEIALKENHLLLNGSMDGMDAASARNLADRMNLYKIGGLLFLPPADL
                     DEFLTCVALFGTPPAVLAAKGGFEAALKQVGLRSIQAVEVAYRRVTDDDPPVESTITA
                     SPSTVTANVEDLVAAWARDFSMPHAVPEEPVIPAVVQKDRIQRLATLLRETATMLEND
                     VGLDSDQRQQQIREAIARIRAALTEVTLESERHITALAEQVNADRQTIASIESAARRR
                     GLGIRLTRAELVERYAELNQEIVQPLTVSTGVIDMLGSGKAGPLNASQRDMLRLASES
                     VERVNQLVAYMCRIAGLPTSLTPDMDMIADTYR"
     gene            complement(1902..2246)
                     /locus_tag="PK576_10630"
     CDS             complement(1902..2246)
                     /locus_tag="PK576_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE62081.1"
                     /translation="MKKNRKGFTLVEIMTVVAVIGILAVIAIPNYLLSRKKSRQNACI
                     VNLKQIQNAKDQSLMANGGFTANDLFGPDKYIKVEPKCPAGGVYTIGDADANPSCDYT
                     AAAGYEHKLPTD"
BASE COUNT          461 a          650 c          739 g          469 t
ORIGIN      
        1 acctcacggt gagaggcaac ccgacctttg ccgatgtggt atcgttcttc agctccgcag
       61 gcatcgatat caaagccggg aacatcgatg gctcctacga aatgtttgag aatcagccgc
      121 cgcaggcgat cgtgaccgca gtgctgatta cggctatcaa tgcccttaag gagcatgcgc
      181 ggaaaaacgg cgcggtctac gcctcgggtg ccgagattcc ggacgcgccg cccggagggg
      241 tcgcgtactg cacgggcggc tcgaaaggtt ggagcgggaa gggcaaaggc tgcttcatct
      301 ttgaaggcga tgtgagcttc cagggcagcg gtatcgacat agaatccgtc aacaactatc
      361 cggcgttgat cgtgctgagc gcttccgagg tcaaattcaa cgcggacgcg gtaatccacg
      421 gtgcgctcct gttcccgaac gcgagcttca cggtcaatgg tcacgcggag atccacggtg
      481 tgatcctggt cggccagtct gcacgtatca acggcacggc cgatctctat ccgggcgaag
      541 gcggccgggg ctttgaggtg ccgccggaag agcgtgttaa agacaacgtc gtcatcaccg
      601 cgtggcatta agccggacca tgtagaggat gttgtgcgag gggcgtaatg gcatacgcga
      661 agataccaga aaaagaagta tcgctgctgg tgagggcctt agggctgctg gtgaatcagg
      721 ccggcgttta cgggcctacg cacaatgtca cccagcgcgc agcgcgcagt gtgttcgcgg
      781 agttggatga gattgtcggg cgttacggcg cggtcgaaat cgcgttgaag gagaaccacc
      841 tgctcttgaa cggctcgatg gacggcatgg atgccgcgtc agccaggaat ttggctgacc
      901 gcatgaacct gtacaagatc ggcgggttgc tctttttgcc gccagccgac ctcgatgagt
      961 tcctgacctg tgtcgccctg ttcggcacac cgcctgccgt gctggcggcg aagggcggtt
     1021 ttgaggccgc gctgaagcag gtcggcctgc gtagtataca ggccgtggag gtggcctacc
     1081 gacgtgtcac agatgacgac cctcccgtgg agtctacgat caccgcgtcc ccgtcaaccg
     1141 tcaccgccaa cgttgaagac ctggtggccg cctgggcacg cgatttcagc atgccgcacg
     1201 cggtacctga agagcccgtg attccggccg tggtacaaaa ggaccgcatc cagcgactgg
     1261 cgaccctgct gcgtgaaacc gccacaatgc tggaaaacga cgtggggctg gacagcgacc
     1321 agcggcagca acagatccgg gaggccattg cccggatccg tgcggcgttg acagaagtca
     1381 ctctggaatc ggaacgtcac atcaccgctc tggcggaaca ggtaaatgcc gaccgccaga
     1441 ccatcgccag catcgaatcc gccgcgcggc ggcgcggcct cggcatccgg ctgacgcgcg
     1501 ccgaactggt ggagcgttac gcggagctga accaagagat cgtccagccg ctgaccgttt
     1561 cgaccggtgt gatcgatatg ctcggttccg gcaaggcggg gccgctcaac gcctcgcagc
     1621 gtgacatgct gcgcctcgcc tcagagagtg tcgagcgcgt caaccaactt gtcgcctaca
     1681 tgtgccgtat cgcgggcctg cccaccagcc tgacgcccga catggatatg atcgccgata
     1741 cctaccgctg atcgcgcgga gcgcgatcgg cggagttggg agttgggatt taggagttag
     1801 gagttaggat ttaggagttg ggagttggga gtttaacgca gaggcttttt ggggggcact
     1861 gctcgggttt tttatccaca gatttttatt gccgcctgtc gtcagtcagt tgggagtttg
     1921 tgctcataac ccgcagcagc ggtataatcg caagatgggt tggcatccgc atcgccgatg
     1981 gtgtagacgc cgccagccgg gcatttcggc tcgaccttga tgtatttgtc gggcccgaac
     2041 agatcgtttg ccgtgaaccc gccgttggcc ataagcgact gatccttcgc gttctggatc
     2101 tgcttgaggt tcacgataca ggcgttctgg cgcgactttt tgcgtgacaa gaggtaattc
     2161 gggatggcga tcacggccaa gatgccgatg accgcaacca cggtcatgat ttcaacgagc
     2221 gtgaaaccct tgcggttctt tttcatgggc gtcccttctt tcctctttag ctcgggcaaa
     2281 atctgtgcca agagagtgac atgagcaaga tgcctgtgc
//
LOCUS       DAOSLE010000238         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_40758_length_2282_cov_7.410418, whole genome shotgun sequence.
ACCESSION   DAOSLE010000238 DAOSLE010000000
VERSION     DAOSLE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40758_length_2282_cov_7.410418"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2282
                     /locus_tag="PK576_10635"
     CDS             <1..>2282
                     /locus_tag="PK576_10635"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HOE62082.1"
                     /translation="ADMPPGALAWPTLTSASGLRARWSLDYYGSREKGFAKQWPVLVA
                     IVDSGGVSVTTEAWVRGVLMSEEWQEWLDATNILLVWWDRAKMSRTKWDDVAFQFAPG
                     EGGIALPQMVLFDTNGLKVDQFLAVSDGGDNLSRAEAFIDRLDNAMDWEGLQTGPGVV
                     GFTASSQAVSATTNAVTVTVKRSGGASEGAQTFRVFTESGSETNAAVAGTHYEAVNTP
                     VSWSAGESGERTVTVKLLDSVASSVTQRVFYVKLEKGAGATAALGTEVHKVTVNYAAG
                     VIGFTTNALTVYAGSPYAEVAVRRSGGVLGVQSVRMRTAALPNGPAQGGALFEKVETN
                     LVWTSGELGEKRVRIPLKGDALDWRAPLTGQVFYAALSPAATNVTARLSITNQAVTLR
                     GSVTPTGDWKYGGTGVWSVVSNTGGQASALTWSTRQGGWLTFAWQKTSPGGTFAAALP
                     GVVTNTAATEVTNRVALAQGANVVWMLTNCTATVKLLEWMPVEVQMLAPEDGTKATNK
                     VVTFSWMAPPGVTSYLYYARSPIPDPLPKYVGGIETNHVSYTNATPGIVEWHVEMEIR
                     GDSQTVARVVGPTWSVNFLKQREVPVAVPPPIADKASPNIVLYQYVPARIPTAERLEA
                     KAARAGRRYTARGLPSGLKIDASSGVIAGTPKRAGTFYAMVKTTTAGMVSSVELEIEV
                     LPCPAFALGEFQGVVLDDASRVRGALALKVSKSGALSGKLESGGRSQSLRGAWVDGNG
                     QARTVRLSAGRVEAMVLTLTP"
BASE COUNT          408 a          597 c          848 g          429 t
ORIGIN      
        1 gcggatatgc cgcccggcgc gttagcctgg ccgactctga cgtcggcttc cggtctgcgc
       61 gcgcgttggt cgctggatta ctacggatca cgtgaaaagg gattcgccaa acagtggccg
      121 gtgctggtgg cgattgtcga ctcaggcggg gtgtcggtga caaccgaggc gtgggtgcgc
      181 ggtgtgttga tgtccgagga gtggcaggag tggctggatg ccacgaacat tctactggtc
      241 tggtgggatc gcgccaagat gtcacggacg aagtgggatg atgtggcctt ccaatttgcg
      301 cccggcgagg gcggcatcgc gctgccgcaa atggtgctct tcgatacgaa cgggctgaag
      361 gtcgaccagt ttctcgcggt cagcgacggg ggtgataacc tatcgcgcgc cgaagcattc
      421 attgatcggc tggataatgc gatggattgg gaggggctcc aaactggacc gggggtggtt
      481 ggatttacgg cctcgtcgca ggctgtttcg gcgaccacca atgccgtgac ggtgacggtg
      541 aaacgttcgg gtggcgcgag cgaaggggca cagaccttcc gcgtgttcac ggagtcaggg
      601 agcgagacga acgcggcggt tgcggggacc cactatgaag ccgtgaacac cccggtgagt
      661 tggtctgccg gggagagcgg cgagaggacg gtcactgtga agttgttgga cagtgtcgcc
      721 tcctctgtga cccagcgtgt gttttatgtg aaattggaaa aaggggcggg cgccacggcg
      781 gcgttaggga cggaggttca caaggtcacg gtcaactatg cggccggcgt gatcggcttt
      841 acaacgaatg cgctgaccgt gtatgcggga agcccctacg ctgaggtggc ggtccggcgg
      901 tccggcgggg tgctgggcgt gcaaagtgtc cgtatgcgca cggctgcgtt gccgaacggt
      961 cccgcgcaag ggggcgccct ttttgagaaa gtggagacca atctggtctg gacttcaggc
     1021 gagttgggcg agaaacgggt gcggattccg ctgaaagggg atgcgctgga ttggcgggca
     1081 ccgttgacgg gacaggtttt ctatgcggcg ctgtcgccgg cggcgaccaa cgtgacagcc
     1141 cgactcagca tcaccaatca ggcggtcacg ctccggggtt ccgtcacgcc gacaggcgac
     1201 tggaagtatg gcggtaccgg cgtgtggagt gttgtttcga atacgggcgg ccaggcctcg
     1261 gcgttgacgt ggagcactcg gcaagggggt tggctcacct tcgcgtggca gaaaacttct
     1321 cccggtggga cgtttgcagc ggcgctgccc ggcgtggtca ccaacacggc tgcgacggag
     1381 gtgaccaacc gcgtggcgct ggcgcaggga gccaatgttg tgtggatgct caccaactgc
     1441 acggcaacgg tcaagctgct ggagtggatg ccggtggagg tgcagatgct cgcgcccgaa
     1501 gacggcacga aagcaaccaa caaggtggtg acgttctctt ggatggcccc ccccggagtg
     1561 acctcctatc tgtactatgc gcgatcaccg atcccggatc cgctgccgaa atatgtcggc
     1621 ggcatcgaaa ccaatcatgt gtcgtatacg aatgccacgc cgggcattgt cgagtggcat
     1681 gtggagatgg agatacgcgg ggattcgcag acggtcgcgc gggtggtggg gccgacctgg
     1741 tcggtcaact tcctgaagca aagagaggtg ccggtggcag tacccccgcc gattgccgac
     1801 aaggcatcgc cgaatatcgt gctgtaccaa tacgtgccgg cccgtattcc gacggcagag
     1861 cgtctggagg ctaaggccgc gcgcgcggga cgccgctaca cggcgagggg gctgccgagc
     1921 ggattgaaaa tcgatgcgtc gtccggcgtc attgccggaa cgccgaagcg tgcgggcaca
     1981 ttctatgcga tggtcaaaac gacgacagcc gggatggtgt cctccgttga attggagatc
     2041 gaggttctgc cctgtccggc gtttgcgctg ggtgagtttc agggggtggt gctggatgac
     2101 gcaagccggg tgcgtggtgc gcttgcgctc aaggtgtcga agagcggcgc gttatcgggc
     2161 aagctcgaaa gcggcggtcg gagccagtcg ctgcgcggcg cgtgggttga cgggaacgga
     2221 caggcgcgga cggtcaggtt gtcggccggt cgtgtcgagg cgatggtctt gacgctcact
     2281 cc
//
LOCUS       DAOSLE010000239         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_41004_length_2272_cov_7.380695, whole genome shotgun sequence.
ACCESSION   DAOSLE010000239 DAOSLE010000000
VERSION     DAOSLE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41004_length_2272_cov_7.380695"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1141
                     /locus_tag="PK576_10640"
     CDS             149..1141
                     /locus_tag="PK576_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE62083.1"
                     /translation="MQYRKLGSSEIQVSAVALGTWSIGGGPWWGKTDDNDSIRAIQAA
                     VDAGITLIDTAPAYGFGRSEEVVGRAIRGRRDQVVVATKCGLWWGDDRGVLHFEQDGY
                     TVRRCLDPRTIRIEVEQSLRRLGVDRIDLMQTHAQAVEPFKTPIAETMACLMELRKEG
                     KIREIGVSNCTPEQMDEYLAAGVIVSNQPRYSLLDRAIEHELLPYCRAKDLAVLVYSP
                     LEQGLLTGKIGMDTSFDPEAFRNKLPWFALDKRRKVLDMLEGWHDLTDKYGCTLSQLV
                     IAWTVSQPGVTAALCGARKEANVLENAKAGDLLLEDADRERMRRDAEALAPSEP"
     gene            1146..2210
                     /locus_tag="PK576_10645"
     CDS             1146..2210
                     /locus_tag="PK576_10645"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE62084.1"
                     /translation="MRTAVQHDTLSDSGGGVILVSCREKIRDADRGERESAEAGGPLP
                     RVLSVPVPADATGRTDVWLTAQLPEFSRARLQALIREGRVTCRGVAVKANASPQPGSV
                     IEICLPAPVAAEPAPEAIPLDTVYEDRDCLVINKPAGLVVHPAPGHAAGTLVNALLHH
                     CRDLVGVGGVQRPGIVHRLDKDTSGLMVVAKHDAAMAGLVRLFQTGEIRKEYLTLVHG
                     TPPQVSGTLRTRIGRHPVHRKKMAVVETGGKEAVTHYTVEKRLGGLSLVRCRIETGRT
                     HQIRVHMLSLGCPVAGDALYGKSAEDKRLPHVPPRQLLHAARLAFAHPLTGVELDFAA
                     PLPADFLPFLEESEGLRIFV"
BASE COUNT          386 a          628 c          819 g          439 t
ORIGIN      
        1 cctgaagagt gacttgggaa tgtgtccctt tccaactccg ccttgctttt cgcatcgtgt
       61 tggcacaaga tagagaggtg ttggcccaat ggattcacgg ggagaggagt ccgttgcggg
      121 cgactggtta atacgggagg atttcggaat gcagtacaga aagttggggt cgtcggagat
      181 tcaggtttcc gcggttgcgc tcgggacatg gtcgatcggc ggcggcccgt ggtggggaaa
      241 gaccgatgac aacgactcga tccgcgcgat tcaggcggcg gtggatgcgg gtatcacgct
      301 gatcgacacg gcgccagcct atgggttcgg acgcagtgaa gaggtggtgg ggcgcgcgat
      361 ccgggggcgg cgcgatcagg tggtggtggc caccaagtgc ggcctctggt ggggtgacga
      421 caggggagtc ctccattttg agcaggacgg atatacggtg cggcgctgtc tcgatccgcg
      481 cacgatccgg attgaggtgg agcagagtct gcggcgtctc ggtgttgacc ggattgacct
      541 catgcagacg cacgcgcagg cggttgagcc gttcaagacg ccgatcgcgg agacgatggc
      601 ctgtctgatg gagttgcgga aggaggggaa aatccgtgag atcggcgtct cgaactgtac
      661 gccggaacag atggatgagt acctcgcggc cggtgtcatc gtcagcaacc aaccccgcta
      721 cagtctgctc gaccgtgcca tcgagcacga gctgttgccg tattgccgtg cgaaggatct
      781 ggccgtgctg gtttactcgc cgcttgagca ggggttgctc accgggaaga tcggcatgga
      841 cacctcgttt gacccggagg cgttccggaa caagctcccc tggtttgcct tggacaaacg
      901 gcgtaaggtg ctggacatgc tggagggctg gcatgacctg accgacaaat acggctgtac
      961 cctctcgcaa ctggtcatcg cctggaccgt ctcccagccc ggggtgacgg ctgccttgtg
     1021 cggtgcccgc aaggaggcga acgtcttgga gaacgcgaaa gcgggtgatc tgcttttgga
     1081 ggatgcggat cgggaacgga tgcggcggga tgccgaggcg cttgcgccat cggagccctg
     1141 agccgatgcg cacggcagtc cagcatgaca cactttcgga ttctggtgga ggcgtgattc
     1201 tcgtgtcgtg tcgggaaaag atcagggatg cggatcgcgg ggagcgcgaa tcagcagagg
     1261 ccggcgggcc gctgccacgg gtgctctccg tgcctgtgcc cgccgacgcc accggacgca
     1321 cggatgtgtg gctgacggcg cagttgccgg agttctcccg cgcgcgcttg caggcgctga
     1381 tccgcgaggg gcgggtgacc tgccgcgggg ttgcggtcaa ggcgaacgcc agtccgcagc
     1441 ccggcagtgt gatcgagatt tgcctgcccg cgccggttgc ggcggaacct gcgcccgagg
     1501 cgattccgct cgatacggtt tatgaggatc gtgattgttt ggtgatcaac aaacccgccg
     1561 gactggtggt ccatccggcg cccggccatg cggcgggaac cttggtgaac gcgctgttgc
     1621 atcattgccg cgatctggtt ggggtggggg gcgtgcaacg gcccggcatt gtgcaccgtt
     1681 tggacaagga cacttcgggt ctcatggtgg tggccaaaca cgatgcggcc atggccggtc
     1741 tggtgcggtt gttccagacc ggcgagatcc gcaaagagta tctgactctg gtacacggca
     1801 cgccgccgca ggtttccggc acgcttcgga cgcggatcgg gcgtcatccg gtgcaccgca
     1861 aaaaaatggc ggtggtggaa acagggggga aagaggcggt gacgcactat acggtcgaaa
     1921 agcggttggg cgggctttcg ctggtgcgct gccgcatcga aaccggccgt acgcaccaga
     1981 tccgcgtgca catgctgtcg ctcggttgtc cggtggcggg ggacgccctg tacgggaagt
     2041 ccgctgagga taagcggttg ccgcatgtgc ccccccgtca actgctgcac gccgcgcgtt
     2101 tggcgttcgc gcatcccctt acgggcgtgg agttggattt tgccgcgccg ttgccagcgg
     2161 atttcctgcc gtttcttgag gaatccgaag ggctacgtat cttcgtgtga gaaacagccc
     2221 tgccgcacgc ttcggtgcgg cccctgcctg ctgcgcaggc ggtcgaaccc gt
//
LOCUS       DAOSLE010000240         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_41599_length_2248_cov_8.625627, whole genome shotgun sequence.
ACCESSION   DAOSLE010000240 DAOSLE010000000
VERSION     DAOSLE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41599_length_2248_cov_8.625627"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2248)
                     /locus_tag="PK576_10650"
     CDS             complement(<1..>2248)
                     /locus_tag="PK576_10650"
                     /inference="COORDINATES: protein motif:HMM:NF039815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62085.1"
                     /translation="MCDGWEVMRGLNPNDSRDGLNAMMDNPDGDYMAYALVPRLLVTL
                     ENGVQVLAPAAAVVSAGEALGEVEGLTIPYRYGDDTAPIAVGRPATGLAEEVVGSVEP
                     IDAVIVHNQVMQEFGFDPRTAWAGTVNPLSNPQRFPEWVAGLTAENVITAPNTRRFTS
                     KDEYLLMKYMAELRLNGCTGAIGDGNAAVKTADWSIYSTHPKTPDSDVVWGDGGAVVK
                     TDRMPDGWELYVSLPPGSEGTETDMLISPWDPYDGDWTFVGEDNLHNNREFHGTDCSA
                     GYTNVALYGVYPGDTPVVTIVRPESDALWINKFWPSNPYAGDTDGDGLKDDAEMTFIY
                     GTPVDNGTTCTPGGGLNPCSVDTDSDQLPDAWEHAFAATATPVSPLATTNGMDGTVSD
                     YREDWDRDGLLNYQEYWVQAVRSLRYDFTSNDIPMDGSAGARALFTPVTNAWDFSMYP
                     FGDANPPLWILLPVGPAKLFVSCDPRDPDTDGDGMDDFYEMFHGLNPILGEITLQEKG
                     DRIREAYKDKGRYTIDCLDNAWGANLPFDFVTYPWLAGLDEADVDADGLRNFEEQLQP
                     DTAAPACYNTDPSPLWLTDDSSPESVTARFYGFDGMFFWPGNNETISRMYLTGYKMFS
                     YEMNEGYDTDNDGVSDKAELMQTATTQSDPQDHDDPRRRQAIWFSGENSAAQSFSVAP
                     VREYSLRSFTVELWACPERVDREQVLIERPVIYPPSDSSTSGNVVRLNFRIGIKADGR
                     VYAMFQNAG"
BASE COUNT          388 a          728 c          712 g          420 t
ORIGIN      
        1 cccgcattct ggaacatggc gtacacccgg ccgtcggcct tgatgccgat gcggaagttg
       61 agccgcacca cattaccgga ggtgctgcta tcactcgggg gatagatcac cggacgctcg
      121 atcagcacct gttcgcggtc cacccgctca ggacaggccc acagctccac cgtgaagctg
      181 cggaggctgt actcccgcac cggagcgacg ctgaaggatt gcgcggcgct gttctcgccg
      241 ctgaaccaga tggcctgacg ccgccgcgga tcgtcgtggt cctgcgggtc ggactgtgtg
      301 gtggcggtct gcatcagctc cgctttgtcg ctgaccccgt cgttgtccgt gtcgtacccc
      361 tcgttcatct cgtaagagaa catcttgtag cccgtcaggt acatcctgga gatcgtctcg
      421 ttgttaccgg gccagaagaa catgccgtcg aacccataga accgggccgt cacgctctca
      481 gggctgctgt cgtcggtcag ccacagcggc gagggatcgg tgttgtagca ggcgggcgcg
      541 gccgtgtcgg gctgcaactg ctcctcgaaa ttacgcagac cgtccgcgtc gacatccgcc
      601 tcgtccaaac ccgccagcca cggataggtg acgaaatcga agggcagatt cgcaccccac
      661 gcattgtcga ggcagtcgat cgtatacctg cctttatcct tgtatgcctc gcggatacgg
      721 tcacccttct cttgtagcgt aatttcgccg agaatcgggt tgagcccgtg gaacatctcg
      781 tagaagtcat ccatgccgtc gccgtccgtg tccggatcgc gcgggtcgca ggagacgaag
      841 agcttggccg gccccaccgg aagcaggatc caaagcggcg gattggcgtc gccgaaggga
      901 tacatgctga aatcccatgc gttggtcacc ggcgtgaaga gcgcgcgggc accggccgaa
      961 ccgtccatcg ggatgtcgtt gctggtgaag tcgtaacgca gactgcgcac ggcctgaacc
     1021 cagtactcct gatagttgag cagaccgtcg cggtcccaat cctcccggta atcggaaacc
     1081 gtgccgtcca tgccgttggt ggtcgccagc ggggacaccg gcgtcgcagt ggcggcgaag
     1141 gcgtgctccc acgcatcggg gagctgatcg gaatcggtgt ccaccgagca ggggttcagg
     1201 ccgccgccgg gggtgcaggt ggtgccgttg tccacaggcg tgccgtagat aaaggtcatc
     1261 tcggcatcat ccttcaggcc gtcgccgtcc gtatcgcccg cataggggtt ggagggccag
     1321 aatttgttga tccagagagc gtccgattcg ggccgcacga tggtcacgac cggggtgtcc
     1381 cccggataca ctccatacag cgcgacgttg gtgtacccgg cggagcagtc cgtaccgtgg
     1441 aactcgcggt tgttatggag gttgtcctcg ccgacaaagg tccagtcgcc gtcgtacgga
     1501 tcccacggcg agatcagcat atcggtttcc gtgccctcag accccggcgg cagggagacg
     1561 tacagttccc atccgtccgg catgcggtcg gtcttgacca ccgcgccgcc gtcaccccag
     1621 accacgtcgc tgtccggtgt cttcgggtgg gtggagtaga tcgaccagtc cgcggtcttc
     1681 acggccgcgt tgccatcgcc gatggcgccg gtgcaaccgt tgagacgcag ctcggccatg
     1741 tatttcatca gcagatattc gtctttggag gtgaagcgcc gcgtgttcgg cgcggtgatc
     1801 acattttcgg cggtcagccc ggcaacccac tccggaaaac gctgcgggtt ggaaagcggg
     1861 ttgacggtgc cggcccacgc cgtgcgcggg tcgaagccga actcctgcat cacctggttg
     1921 tggacgatca ccgcatcgat cggctccacg ctccccacaa cctcctccgc caacccggtg
     1981 gcgggacggc cgaccgcgat cggcgcggtg tcatcgccat accggtaggg gatcgtcagg
     2041 ccttcgacct cgccgagcgc ctcgcccgca gagaccacgg ccgccgcagg cgccagaacc
     2101 tgaactccgt tttcgagggt cacgagcagc cggggcacca gggcgtaggc catgtagtcg
     2161 ccgtccgggt tatccatcat cgcgttcaga ccgtcacgcg aatcgttcgg gttcaagccg
     2221 cgcatcacct cccagccgtc gcacatgc
//
LOCUS       DAOSLE010000241         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_42327_length_2220_cov_9.274827, whole genome shotgun sequence.
ACCESSION   DAOSLE010000241 DAOSLE010000000
VERSION     DAOSLE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42327_length_2220_cov_9.274827"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="PK576_10655"
     CDS             complement(<1..2018)
                     /locus_tag="PK576_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOE62086.1"
                     /translation="MAAGVQAGQVVVAPDGMTPKAALEHIRAAKAQGNKEAWTVIVKP
                     GIYALTETLTFTPADSGTPQAPVTWVGEGAGATISGGGVIQGWKSEADGTWSAPIPKA
                     PDGKPAYFEQLWVNGRRADRARLPNGGQGTASYFKLVKPSISAVTNAAGKVAYIERAT
                     LTNSLAAAIGKIPADEYPYAQMCVVHKWSTARRILRNFDPATQTVETESPQEWRGWVK
                     WNERETLVSFENVRSAFDAPGEWFYDMKAGCVRYWPLAGETIATVVAVAPSAKLSQLI
                     AFQGDPDKGEFVHDIAFRNLTFAYSDVTPTVGRAVNGPTESWQHQAASGSDGLVTAEG
                     THRVSWDKCVVAHTGNYAFRFNDGCVSNRITNCMIEDLGAGGIWMGASGGHVAKGEVL
                     KRRVIKTLAPRSTAFNVIDNCVIRKGGLFNPEGTGVTLTHVSDSKVTHCEIYDFYYTG
                     VSVGWTWGFHGSVAQRNEVAFNKIYDLGKGIMSDMGGVYTLGTSYGTTVHDNVIHDVW
                     AYSYGGWALYTDEGSEGIVMERNLCWNTTDGGFHQHYGTGCIIRNNVFAWNRKLGAVR
                     TARQVVQDIPCTLHFVNNLVVVREGPLVGRGPRGVGGIWAGNLWYDYSGKPELDGLAW
                     EAWQACGKEVGGKYADPLFENADACDFRLKPESPAFALGFKAWDYTRAG"
BASE COUNT          378 a          785 c          626 g          431 t
ORIGIN      
        1 cctgcccgcg tgtaatccca cgctttgaac ccgagcgcga atgcggggga ttcgggcttg
       61 agccggaagt cgcacgcatc ggcattctcg aagagcggat cggcgtactt gccgccgacc
      121 tctttgccgc atgcctgcca cgcttcccac gcgaggccgt cgagttccgg cttgcccgaa
      181 taatcgtacc acaggtttcc ggcccagatg ccgcccacgc cgcgcgggcc gcgcccgacg
      241 agcgggcctt cgcgcaccac gaccaggttg ttcacgaagt gcagcgtgca ggggatgtcc
      301 tgcaccacct gacgcgccgt gcgcactgcg ccgagcttcc ggttccacgc gaacacgtta
      361 ttgcggatga tgcagcccgt accgtaatgc tggtggaacc cgccatccgt cgtgttccag
      421 cacaggttgc gctccatcac aatgccctcg gaaccctcgt ccgtgtacag cgcccacccg
      481 ccgtaggagt aggcccacac gtcatgaatc acgttgtcat gcaccgtcgt gccgtagctt
      541 gtgccaagcg tgtatacgcc gcccatatcg ctcatgatgc ccttgccgag gtcgtagatc
      601 ttgttgaacg cgacttcgtt gcgctgcgcc acgctgccgt gaaaacccca cgtccaaccg
      661 accgagacgc ccgtgtagta gaaatcgtag atctcgcaat gcgtcacctt cgagtccgac
      721 acatgcgtga gcgtcacacc cgtgccttcg ggattgaaca ggcccccctt gcggatcacg
      781 cagttgtcga tcacgttaaa cgcggtcgag cgcggcgcaa gcgtcttgat cacccggcgt
      841 ttgaggacct cgcccttggc gacgtggcca ccggaggcac ccatccaaat gccgcccgcg
      901 cccagatcct cgatcatgca gttcgtgatc cggttggaca cgcacccgtc gttgaagcgg
      961 aacgcgtagt tgcccgtgtg cgcgaccacg cacttgtccc acgagacacg gtgcgtgccc
     1021 tcggcggtca cgaggccgtc cgatccgctg gccgcctgat gctgccagct ttccgtcggg
     1081 ccgttcacgg cgcgtcccac tgtcggcgtg acgtcactgt acgcgaacgt caggttgcgg
     1141 aacgcgatgt catgcacgaa ttcgcccttg tccggatcgc cctgaaacgc gatcaactgg
     1201 ctcagcttgg cgctcggcgc gaccgcgacg acggtcgcga tggtctcgcc ggccagcggc
     1261 caatagcgca cacagcccgc cttcatgtca tagaaccatt cgcccggcgc atcgaaggcg
     1321 ctccgcacgt tctcgaaaga gaccagcgtc tcgcgctcgt tccattttac ccaccccctc
     1381 cattcctgcg gggactcggt ctcgaccgtc tgcgtggcag gatcgaagtt gcgcaggatg
     1441 cggcgcgccg tgctccactt atgcaccaca cacatctgcg cgtacggata ctcgtcggcc
     1501 ggaatcttcc cgattgctgc ggcgaggctg ttcgtcaacg tggcgcgttc gatgtaggcg
     1561 acctttccgg ccgcgttcgt cactgcggag atgcttggct tcacgagctt gaaataactc
     1621 gccgttccct gaccgccatt cggaagacgc gcacggtcgg cacgacgtcc gttaacccat
     1681 aactgctcga aataagcggg cttcccgtcg ggcgccttcg ggatcggcgc ggaccatgtg
     1741 ccgtccgcct cgcttttcca cccttggatc accccgccgc cggaaatcgt cgcgcccgca
     1801 ccctcgccca cccatgtgac cggtgcctgc ggcgtgccgg aatcggctgg cgtgaacgtg
     1861 agcgtctccg taagcgcgta gatgccgggt ttcacgatca ccgtccacgc ctctttgttt
     1921 ccctgcgcct tggccgcgcg gatgtgctcc agcgcggcct ttggcgtcat cccgtccggg
     1981 gcgaccacca cttgacccgc ctgaacaccg gctgccacgg cccaaattcc gaaaagacac
     2041 gcgaacctcg ttttgtttcc cattccaagc ctccccgaca tgttgtgttt ttccgactga
     2101 caataaaacg catcccccgt attccgcagg acgcgcttcc tcccgcaggc tcccccggca
     2161 acaaaccggt tcccatgatt gccgcctatt atgcggggga tcgacgaaac gagtcaaccc
//
LOCUS       DAOSLE010000242         2190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_43111_length_2190_cov_8.264169, whole genome shotgun sequence.
ACCESSION   DAOSLE010000242 DAOSLE010000000
VERSION     DAOSLE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43111_length_2190_cov_8.264169"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK576_10660"
     CDS             <1..215
                     /locus_tag="PK576_10660"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOE62087.1"
                     /translation="EAVQRERLMRSRGLTEEEARLRIAAQWPVAEKAARSHYVVYNES
                     DVAALAREADRIVRLLAASRMDHGGT"
     gene            299..1936
                     /gene="rho"
                     /locus_tag="PK576_10665"
     CDS             299..1936
                     /gene="rho"
                     /locus_tag="PK576_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOE62088.1"
                     /translation="MSTEHEASEQNVSQNSGGQSDGAAQAAPSQQAGGGGGKWTPRFT
                     PRPPSSARPSSSGRRDGRSSRGGRGRARPQKHLSRGGSARHSSKSQHLPKGLPADYDQ
                     PDMPPSPAPQNGEGAPDTAHPTETLPPVYLTDLQKQEVAELLELLPNANSEDLAALRK
                     HEIIFEVIRSYLRRGGTVITSGVLEILPDGYGFLRSPKSNYLQCPEDVYVSPSQVRRF
                     GLRTGDVVEGPVRDPREKERFFALGRVDMMNGKPPVEARRTVHFENLTPLFPDRRIQL
                     ETSPDEISMRVMDIFTPIGFGQRGLIVAPPRTGKTVLLQKVANAISANHPDAMLIILL
                     IDERPEEVTDMQRNTKAQVLSSTFDEAPERHVQVAEMVIEVAKRQVENGRDVVILLDS
                     ITRLARAYNTVQPHSGKILSGGVDANALHKPKRFFGAARNIEKGGSLTIIATALVDTG
                     SRMDEVIFEEFKGTGNMELCLDRQLVDKRVFPAINIEKSGTRKEELLLYPEELNRTWI
                     LRRALNGVPPVEVMEMVVKRIKNTKSNAEFLVSIKEQ"
BASE COUNT          437 a          632 c          734 g          387 t
ORIGIN      
        1 gtgaggcggt gcagagagaa cggttgatgc gctctcgggg actgacggaa gaggaggccc
       61 gcctgcgcat cgctgcgcag tggccggtag ccgagaaagc cgcccggtcg cactacgtgg
      121 tttacaatga gtccgatgtt gcagcgttgg cgcgggaagc ggaccggatc gtccgcctgc
      181 tcgctgcctc acgtatggat catggcggca cgtaacgagt tgttgacgcc cttccccctt
      241 tagtataggg agggcagagg tgaggattgc gggctttgcc cgcatgagga gagaaccaat
      301 gagtacagag cacgaggctt ccgagcagaa cgtttcgcag aattcgggtg gccaatccga
      361 tggcgccgcg caggccgcgc cgtcccaaca agcgggagga ggggggggga aatggacgcc
      421 gcgattcact ccgcgtccgc cttctagcgc acggccttct tcgagcgggc gccgggatgg
      481 ccgtagtagc cgcgggggcc ggggccgggc acggccgcag aagcacttgt cgcgcggtgg
      541 ttcggcccgc cactcgtcga aatcgcaaca cttgcccaag gggttgcctg cggattacga
      601 tcagccggac atgcctccta gtcccgcgcc gcagaacggc gaaggtgcgc ctgacaccgc
      661 gcatccgacg gagacgctgc cgcccgtgta cctcaccgac ctccagaagc aagaggttgc
      721 cgagttgctg gagctgttgc cgaatgccaa ctccgaggat ctggcggccc tgcgcaagca
      781 cgaaatcatt ttcgaagtca tccgcagcta tctccgccgt ggcggcacgg tgatcaccag
      841 cggtgtgttg gaaatcctgc cggatggata cggctttctc cgttcgccca agagcaatta
      901 cttgcagtgt ccggaggatg tctatgtctc gccttcgcag gtgaggcgct tcggattgcg
      961 gaccggcgac gtggtggagg ggccggtgcg cgacccgcgg gaaaaggagc gtttctttgc
     1021 gctgggccgc gtggacatga tgaacgggaa accgccggtg gaggcgcggc gtacggtgca
     1081 tttcgagaat ctgacgccgc tgtttccgga ccggcgcatc cagttggaaa cctcgccgga
     1141 cgagatttcg atgcgcgtca tggatatttt cacgccgatc ggattcggtc agcgcgggct
     1201 gatcgtggcg ccgccgcgca ccggcaagac cgtgttgctg caaaaggtgg ccaacgcgat
     1261 ctccgctaac cacccggacg cgatgctgat catcctgctg atcgacgagc ggcccgaaga
     1321 ggtcaccgac atgcagcgca acaccaaggc gcaagtgttg agttcgactt ttgacgaggc
     1381 tccggaacgg cacgtgcagg tcgctgaaat ggtcatcgag gtggccaaac gccaggttga
     1441 gaacggccgg gacgtggtga tcctgctgga cagcatcacg cgtttggcgc gcgcctacaa
     1501 cacggtgcag ccgcacagcg gtaagattct ctcgggcggg gtggatgcca acgcgttgca
     1561 caagcccaag cgctttttcg gcgcggcgcg aaacatcgaa aagggcggca gtctgaccat
     1621 catcgccacg gcgttggtgg atacgggcag ccgcatggac gaggtcattt tcgaggagtt
     1681 caagggtacc ggaaacatgg aactctgcct ggaccgccaa ctggtggaca agcgcgtctt
     1741 cccggcgata aacatcgaga aatccggcac gcggaaagag gagttgctgc tctatcccga
     1801 agagctgaac cgcacctgga ttctgcggcg tgccctgaac ggcgtgccgc cggtcgaggt
     1861 gatggaaatg gtggtcaagc gcatcaaaaa caccaagagc aacgcggagt ttctggtgag
     1921 catcaaggag cagtgatcac ggcatcggga tcgcttctcc gtccccgtac acattgactt
     1981 gcgacccgcg acgttcgacc cgcgacactt gggctgtttg gcaggacgac ggatccgccg
     2041 ttacgggcgg ggacgcccga cttcaggcca acgcgggcag agcccgcaaa gcggcacaaa
     2101 acctaaaaaa accttcgtgc ttttgtgggg ttaagaactg cgcggaggga cgggttttat
     2161 ttgcgcattc ccatcccgat gcggacatgt
//
LOCUS       DAOSLE010000243         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_44596_length_2136_cov_9.996636, whole genome shotgun sequence.
ACCESSION   DAOSLE010000243 DAOSLE010000000
VERSION     DAOSLE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44596_length_2136_cov_9.996636"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK576_10670"
     CDS             <1..779
                     /locus_tag="PK576_10670"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOE62089.1"
                     /translation="KPENGAVTVEITSADANKWPINLLAKGDIADRIWENILNVIGLP
                     MEYQEEVVDSWYDWLDTDSTITGRSGAEDEYYSDLDKPYKARNGPISSVAELEMVRGI
                     RERPAIFNGGILNPEERNKKAQVRIQPGLKAFFDIYGETVKINVNSAELEVLLTVPGI
                     DGDSLLANAIIEERRTGANRVTSGDASAESLLFKDWNDLNTRIPGGVPTEAEPFLAYA
                     PQKYFEIAVTGEAGGISHQIKAVAIVENDRVRYLRWREDP"
     gene            819..>2136
                     /locus_tag="PK576_10675"
     CDS             819..>2136
                     /locus_tag="PK576_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62090.1"
                     /translation="MTKSDLIALLAEGGVLRGVKFAPRGRDGWTRTGGGVWPLETPDA
                     EVTVDEDEIDAAAPPAVADETVVESDKPMTRALVAARSALGARDIVLALPLSQVLVRV
                     FKMPLEVRDDVADAVALQMDKLSPFPGEELSVGCEVLSETESHLWVFAAALPAAVFEE
                     LGAALDEARLQAVRTDVAMLGWLRSLSGPCQLMRTGRRVVLMDPDGSWDLVVLDHGVP
                     VLARGLGGAVSAEDVVREMTLSLLNAEMEAGSGAVAEVLVIAQQVPDQELVEKLRDLT
                     GAEVRHVVPPTPDGGVEGVALRAGEGAALDLTPQVWRDQTKALRVRKQVMTGVGIALV
                     VWAGLMGSLFTGPAVYKQLTAQVRKHSRAHAKAYKQVSDTRERVNLIESYTDRSRSSL
                     EMLRLISSVLPQGITLIGFTYRRDEGVKVSGEADQPTVVYDFKNAVT"
BASE COUNT          394 a          558 c          759 g          425 t
ORIGIN      
        1 acaaacccga aaacggcgcg gtgacggttg agatcacctc ggctgacgcc aataaatggc
       61 caatcaatct gctggccaaa ggggatatcg ccgaccggat ctgggagaac atcctgaatg
      121 ttatcgggtt gccgatggag tatcaggagg aggtggtcga cagttggtat gactggctgg
      181 acactgacag cacgatcacc ggacggagcg gtgcggaaga cgagtattat agcgatctcg
      241 acaagccgta caaggcgcgg aacgggccga tctcctcggt cgccgaattg gaaatggtca
      301 ggggtatccg tgagcgtccc gccattttca acggtggcat cctgaacccc gaggagagga
      361 ataagaaggc gcaggtccgc attcagcccg ggcttaaggc tttttttgac atctatgggg
      421 aaactgttaa gatcaatgtc aattccgctg aactggaggt gttgttgaca gtgccgggca
      481 ttgatggcga cagtttgttg gcgaatgcga tcatagagga gcggaggacc ggcgccaacc
      541 gggtcacatc aggagatgcg tcggcagaaa gcttgctgtt taaagactgg aacgatctga
      601 acacgcgcat ccccggcggt gtcccgactg aggcagagcc ttttttggct tatgcgcccc
      661 agaagtattt tgaaatcgcg gtgacgggcg aggcgggagg catctcgcac cagatcaagg
      721 cggtggcgat tgttgagaac gaccgggtgc gttatctgcg ctggcgtgaa gatccgtaac
      781 taattaactt ggattgcggg ctctgcccgc atgttgatat gacgaagagc gatttgatag
      841 cgttgttggc ggaaggcggc gtgctgaggg gcgtgaagtt cgctccgcgc ggacgtgacg
      901 gctggacgcg caccggtggg ggcgtttggc cgcttgagac gcccgatgcc gaagtgacgg
      961 tcgacgaaga cgagatcgat gcggccgccc cccctgcggt tgcggatgag actgtagtcg
     1021 agagtgataa gccgatgact cgggcgctgg tggcggcccg ttcggcgttg ggagcgcgtg
     1081 atatcgtgct ggcgctgccg ctttctcagg tgttggtgcg cgtattcaag atgccgctgg
     1141 aagttcgcga cgacgtggcg gacgccgtgg cgttgcagat ggacaagctc tcccctttcc
     1201 ccggcgagga gctgtcggtc ggctgcgagg tgttgagcga gaccgagtct cacctctggg
     1261 tttttgccgc tgccctgccg gctgcggttt tcgaagaatt gggcgcggcg ctggacgaag
     1321 cgcgcttgca ggcggtccgc actgacgtcg ccatgctggg ctggctgaga tcgctgagtg
     1381 gtccctgcca actgatgcgc accggccggc gtgtggtgtt gatggatccg gacggttcgt
     1441 gggatctggt ggtgctggat catggtgtgc ctgttttggc gcgcggtctg ggcggagctg
     1501 tgtccgccga agatgtggtc cgggagatga ccctctccct gttgaatgcc gaaatggagg
     1561 ccggcagcgg cgcggtggcc gaggtgttgg tgatcgcgca gcaagtgccg gatcaggaac
     1621 tcgtcgaaaa gctgcgcgac ttgaccggtg ccgaagtccg gcacgtcgtg ccgccgacgc
     1681 cggacggcgg ggtcgagggt gtggcgctgc gtgccggcga gggtgctgcg ctggacctca
     1741 cgccgcaagt gtggcgcgat cagaccaagg cattgcgtgt ccgcaagcag gtcatgaccg
     1801 gcgtagggat cgcgctggtg gtctgggcgg ggttgatggg ctcgctcttc accgggccgg
     1861 cggtctacaa gcaactcacc gcgcaggtcc gcaagcactc gcgcgcgcac gccaaggctt
     1921 acaagcaggt ctccgacaca cgcgagcgcg tcaacctgat cgagtcttac accgaccgtt
     1981 cccgctcgtc gctggagatg ctgcgcctga tctcctcggt cttgccgcaa ggaatcacgc
     2041 tgattgggtt cacgtaccgg cgcgacgaag gggtgaaagt ctcgggcgag gccgatcagc
     2101 cgacggtggt ttacgatttc aagaatgcgg tgaccg
//
LOCUS       DAOSLE010000244         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_45263_length_2114_cov_10.772220, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000244 DAOSLE010000000
VERSION     DAOSLE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45263_length_2114_cov_10.772220"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            632..2038
                     /locus_tag="PK576_10680"
     CDS             632..2038
                     /locus_tag="PK576_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE62091.1"
                     /translation="MDRREFLKGAAVAAIGTGIPFSVRAETSAVRSGQVAYSRTFPVK
                     IETDVMVCGGGSAGVAAAVAARAAGARVFLAEAFTCFGGMGTAARVPVYMQFGDGIRP
                     LAAGFGTRFRERLLKAGAISSNYGSFDFEAVKREYDAVMVESGADFLFHTKVIDVIRE
                     GDEIKHAVCASQSGIWAVKAKVYVDATGNGDVAVQSGVPFEKGDSMGKMMPASLLSSW
                     IGIDWDQWRKGLRSFNAEIDQALADGVFKVPDRHMTGIFKSANGLGNANIGHVYDLDG
                     TDERSLTKGYLRGRESMKEYQRYFSEYLKHGLENIRLIETGALMGVRETRRIMGDYVM
                     TLDDYVKRAVFPDEIGRYAYPIDIHPLSADKEAYARHREEFDRLYKYQKGESYGIPYR
                     ILCAKGVKNLLVAGRCVSTDQKVQASIRVMPACYIMGQAAGFAAAMAADGAGDIRGIC
                     VSELQRKLKNFGAYLPNA"
BASE COUNT          425 a          574 c          667 g          448 t
ORIGIN      
        1 atccgcttcc acacgttgga gtcgtccgaa atcccgatcc ttctggtcac caacgataac
       61 gatttccgcg ccaaagctcg tcggcttggt gcgcaatgcc tgtcggtgcc tgatttcggc
      121 gccttcatcc cgtgagcttc cccaagaatt gaagggcaag cccttgtaat cggttagtga
      181 caggggtcat gcgagtcgaa agcatggttt gtgtgttcgc gcattttctt gagcgttgaa
      241 agttgagtgc aagttaggcg aacggatttg gagcgcggcg gcaagcggta ttccgcgcga
      301 cgccgctttt tgagcatgag tgcaacggca aggtagcgcg caaaagcggt gccgcgaccc
      361 gcgcacagcg cggctcttgt caccgcactc caaatttttc cgccgcatca cgcgcagcac
      421 aacacacttg taaaatcatg tctaaaaaac gcaacccgct tttcaccaga aatgcgcgat
      481 cttcgcaccc cctgtcacca accgattacc aagccctttc tatttacggt tgactgtttt
      541 actctccccc cgcacaatgg acgtcggcta tgggaacctg aattgcagtc tctgctcaac
      601 aggcaaagtg gaatcttgaa aggatacgag agtggacagg cgcgaattct tgaaaggggc
      661 ggcggttgcc gcgataggga ccgggattcc gttcagcgta cgagcggaga catcggctgt
      721 acgtagcgga caggtggcgt attcgcgcac tttccccgtc aagatcgaga cggacgtcat
      781 ggtctgtggt ggcggctcgg ctggcgtcgc ggcggcggtc gcggcacggg ctgcgggggc
      841 gcgtgttttt ctggcggagg cgttcacgtg cttcggcggt atgggtacgg cggctcgggt
      901 gccggtttac atgcagttcg gcgacgggat ccgtccgctc gccgccggtt tcggaacgcg
      961 tttccgcgaa cgcctcttga aggccggcgc gatatccagc aactatgggt ctttcgactt
     1021 cgaggctgtt aagcgcgaat atgacgcggt gatggtcgaa tcgggtgcgg acttcctctt
     1081 ccacacgaag gtcatcgatg tcattcgtga aggtgacgag atcaagcatg ccgtctgcgc
     1141 gtcgcaaagc ggcatctggg cggtgaaggc gaaggtttat gtcgatgcga ccggcaatgg
     1201 tgatgtcgcc gtgcagtcgg gcgtgccgtt cgagaagggc gattcaatgg gcaagatgat
     1261 gccggcgtcg ttgctttcct cttggatcgg tattgactgg gaccagtgga ggaaggggct
     1321 gcggtcgttc aatgccgaaa ttgatcaggc actggcggac ggtgtgttca aggttccaga
     1381 tcgccacatg acgggcattt ttaagtccgc gaacggtctt ggaaacgcca atatcggaca
     1441 cgtctacgat ctggacggta cggacgaacg ttcgctcacg aaagggtatc tgcgtggtcg
     1501 cgaatcgatg aaggaatatc agcgctattt cagcgagtac ctgaagcacg ggctcgagaa
     1561 catccgtctc atcgagaccg gcgcgctgat gggcgtccgc gagacgcgcc gaataatggg
     1621 cgactacgtg atgacgctcg acgactacgt gaagcgcgcg gttttcccgg acgagatcgg
     1681 gcgctacgcc tacccgatcg acatccatcc gttgagtgcg gacaaggagg cttatgcgag
     1741 gcaccgcgag gagttcgacc ggctctacaa gtatcagaag ggcgagagtt acggcatacc
     1801 ctatcgcatc ctctgcgcga agggcgtgaa gaatttgctc gttgccggac ggtgcgtttc
     1861 gaccgaccag aaggtgcagg catcgatccg ggtgatgccg gcgtgctata tcatgggtca
     1921 ggccgccggt ttcgccgccg cgatggcggc ggacggcgcg ggtgatatcc gcggcatctg
     1981 tgtatccgaa ctccagagga aattgaagaa ctttggcgcc tatcttccaa acgcctgatc
     2041 cggcccgccc gagatggctt ccgccttacg cggggaccga cgagacctgc catgcagacc
     2101 attcgcatca ctga
//
LOCUS       DAOSLE010000245         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_45607_length_2103_cov_8.338379, whole genome shotgun sequence.
ACCESSION   DAOSLE010000245 DAOSLE010000000
VERSION     DAOSLE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45607_length_2103_cov_8.338379"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..898)
                     /locus_tag="PK576_10685"
     CDS             complement(95..898)
                     /locus_tag="PK576_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE62092.1"
                     /translation="MNKYLIFAGVAALALTASAGNLTEKIASKHKVKSTDIWYGGQRT
                     VFDFQGYDAWVVEPPAEVKPIEGTPWTWTMQWRTAFVPRTSVPRMLKMGYHHVSVDTF
                     KEHMNEKGLAVSKAFQDYLVKDLGFAPKARLIGMSWGGFFSIRYAATFPENVAKIYLD
                     APLLSFENFSSVPDHWKKTQPEQGWKDDPRMPVNMAKTIAETGIPVLLLYGGVDTVVP
                     PKSNCIRFAEAFRAAGGKPEKLKVVARGSYAHHPHGVEESDNTIIDFFR"
     gene            complement(1235..1762)
                     /locus_tag="PK576_10690"
     CDS             complement(1235..1762)
                     /locus_tag="PK576_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE62093.1"
                     /translation="MNDKILPLLGRRSVRVYRPGEAVSDDELRALLEAAMAAPSARAC
                     DPWRFLVVRERADLDALADLLPYGQMLRQAALGIVVCGDQAQAHRQELSYLLQDCSAA
                     VQNLLVAAHLLGLGAVWLGVHPNEDRVTGIRRQFGVPEAILPIAAISIGRPAETPAPR
                     TRFNPDFVHNGRWRA"
BASE COUNT          442 a          636 c          576 g          449 t
ORIGIN      
        1 atagcgaccc cgaccccgat ttggatgaaa ccatgaaacc agaaaccctg aaaccctgaa
       61 acctgaaacc atgaaatcaa agagagttga agcgttaccg gaagaaatcg atgatcgtgt
      121 tatcgctctc ctccacgcca tgcggatgat gggcgtaaga accgcgggca acgactttga
      181 gtttctcggg cttaccacca gcggcccgga atgcttccgc aaagcggatg cagttggact
      241 tcggcgggac cacggtgtcg acgccgccgt acagaagcag aaccgggatt ccggtctcgg
      301 ctatggtttt cgccatgttc accggcatgc gcggatcgtc cttccacccc tgctcgggct
      361 gggtcttttt ccaatgatca ggaacgctcg aaaaattctc gaaactgagt agcggcgcgt
      421 ccagatagat cttcgccacg ttttcgggaa acgtcgccgc ataccggatc gagaagaatc
      481 cgccccagct cataccgatg agccgcgcct tgggcgcaaa cccgaggtcc ttcaccagat
      541 aatcctgaaa agcctttgac acggcgagcc ccttctcgtt catgtgctct ttgaacgtgt
      601 ccacggaaac gtggtgatag cccatcttca gcatgcgcgg gacgcttgtc cgcgggacaa
      661 aagccgttcg ccactgcatt gtccaggtcc agggggttcc ttcgatcggc ttgacttccg
      721 ccggcggttc caccacccaa gcgtcatatc cctggaagtc gaaaaccgta cgctgaccac
      781 cgtaccagat gtccgtgctc ttcaccttgt gcttggacgc gattttctca gtcaaattcc
      841 cggccgacgc cgtcaacgcc aatgccgcaa cacccgcgaa aatgagatac ttgttcatcc
      901 tgttctctac cttgatgaga gttaaaaccc gttgcagaac atcccggtct caccactgag
      961 cgtcctgctt ttccccgtat gcctcttttt tgtaacaatt ttcggatgac gcggtcaagg
     1021 tcggagtcga ggtgccgaca acacagaacg catccctgtt gcgtggacgc tcccgatcct
     1081 gtccccgttt tcatgaatgt cgacttgcgc cccgcaacat gcgccccgcg acgttcgggt
     1141 tgttgggcaa ggctcctgat tcgactgatc cgtcattgcg ggcggggaca cccgcattcc
     1201 tgactgggta ggggacgcat ccccgaggcg tccgctaagc cctccagcga ccgttatgca
     1261 cgaaatccgg attgaagcgt gtgcggggtg caggcgtttc agccggtcgg ccgatggaga
     1321 tcgcggcaat cggcaggatc gcctcgggca cgccgaactg acggcggata ccggtcacgc
     1381 ggtcttcatt cggatggacg cccaaccaga ccgcgccaag cccgagcaga tgcgcggcaa
     1441 ccagcaggtt ttggacagcg gctgaacagt cctgcaacag gtaggagagt tcttggcgat
     1501 gggcctgtgc ctgatcgccg cagaccacga tgccgagggc agcctgacga agcatctgtc
     1561 cgtacggcag cagatccgcc agcgcatcaa gatccgcgcg ctcacgcacc accagaaaac
     1621 gccacgggtc gcacgctctg gctgacggcg cggccatcgc ggcctccaac agggcacgca
     1681 actcatcgtc gctgaccgcc tcgcccggac ggtaaacacg gacactgcgc cttcccaaga
     1741 gaggaagaat cttatcgttc atggggtcac tcctttctcc attcacatct atgcgcccgg
     1801 cccgaccaca agcggtcttt tcaacgcatc gttctgatca actcaaccgt atcgttaatc
     1861 ggcacgcctg tcaatgatgc cgcaagggtg tccgtaatcg gttcgtggca gaggtgtgcg
     1921 aagacaatac ctaaaaagag cagttgaacc ttcaactctc tttggtttca gggtttctgg
     1981 gtttctgggt ttctgggttt ctggtttcag ggtttcaggg tttcatcggg gtcgggatcg
     2041 ctatcggaat cggaatcgat tgtttaacca cgaacacacg ggatggcact gctcgggttt
     2101 ttt
//
LOCUS       DAOSLE010000246         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_46878_length_2061_cov_8.756231, whole genome shotgun sequence.
ACCESSION   DAOSLE010000246 DAOSLE010000000
VERSION     DAOSLE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46878_length_2061_cov_8.756231"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PK576_10695"
     CDS             complement(<1..220)
                     /locus_tag="PK576_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62094.1"
                     /translation="MSLRFRDIAFPLLFGILTLRAAEQDEVRFKTRTYTPDRTAGHAV
                     YRSEAYTESAAKRPIGKTTEPRDTRGFFG"
     gene            complement(222..848)
                     /locus_tag="PK576_10700"
     CDS             complement(222..848)
                     /locus_tag="PK576_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE62095.1"
                     /translation="MGIVIDHEARKRAIIAKSIQLFSEQGYDGVTYQKIADSCAIART
                     TLYKYFRNKRHIFNTAIWEVSYLLIERFEEILSSRTSTVDRLDRVMRAVLTLLFEQRV
                     ILTVILDYVLAAQRAGQQMDRTISRHTIGLRRILHRLVVEGLRKGELRRINATLATEL
                     LYAQMEAAVLRLTVSQNADYDELSRMLHQTIQNLTVPSAGSRSKNRRA"
     gene            1058..1837
                     /locus_tag="PK576_10705"
     CDS             1058..1837
                     /locus_tag="PK576_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE62096.1"
                     /translation="MGDTVSKELAEARWRKVRTLLRERGILRVEELAAELDVSAATIR
                     RDLAALEAAGGLRRIHGGAIAPDGRDEEPLFDDKTAMSAAEKGRIAEAALRLIRPRDI
                     VYLDGGSTILALARLLIHHTQITVVTNSLRVAQVFSSGGPRMILTGGECRLISQTFVG
                     PLTRSILGQLHLDIAFMGTIGVSAETGMTTTDPAEAYTKEQAMARAARVVLLADSSKF
                     GKTSFVRFGALSALSALISDKRMPAAQRKAFQRAGVETVLV"
     gene            1859..>2061
                     /locus_tag="PK576_10710"
     CDS             1859..>2061
                     /locus_tag="PK576_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62097.1"
                     /translation="MATNNYISTRKPGFKPQKYDLGKIPAYQYKGTLKDEIAAGNITA
                     AQAVDVLEDMLTIREFEEMIVKLR"
BASE COUNT          420 a          646 c          606 g          389 t
ORIGIN      
        1 gcccgaaaaa gccgcgcgtg tccctcggct cggtcgtctt gccgataggg cgcttcgcgg
       61 cggactccgt atacgcctca cttcgataca ccgcgtgacc tgcggtccgg tcgggtgtgt
      121 acgttcgggt tttgaaccgc acctcatcct gctctgcggc gcgcaacgtc aggattccga
      181 acaggagcgg aaacgcgata tcccgaaaac gcaagctcat cttacgccct cctgttcttg
      241 gatcgggaac cggccgacgg cacggtaagg ttctggatgg tctggtgcag catccgcgac
      301 aactcgtcgt aatccgcatt ctgactcacg gtcaagcgca gaacggccgc ctccatttgc
      361 gcgtacagta actctgtggc gagcgtggcg ttgatccggc gcaactcccc tttgcggagc
      421 ccctcgacca ccagccggtg caggatgcgg cgcagcccga tcgtatgccg cgagatcgtc
      481 cgatccatct gctgccccgc ccgctgcgcc gccaacacgt aatccaagat caccgtcaga
      541 atcacgcgct gctcgaaaag cagggtcaac accgcgcgca tcacgcggtc cagccggtcc
      601 accgtactgg tccgggagga gaggatctcc tcaaaccgtt cgatcagcag atacgagacc
      661 tcccaaatcg cggtattgaa gatatgccgc ttgttgcgga agtacttgta tagcgtggtc
      721 cgggcgattg cgcaactgtc ggcgatcttc tggtacgtca ccccatcata tccctgctcg
      781 gaaaagagct ggatcgattt ggcgataatc gcgcgtttgc gcgcctcgtg atcgatgacg
      841 atacccatgc ggacctcttg ccaaccggtg cggccccgta cgaaaaccgt agcaaacgtg
      901 accgcccgtt acattcattt gattgtcgat taaacgcgtg gtgacgtcaa gccccgactt
      961 gacgaaaacc gaaaatgtgt cattccgcgc atccgcgctt gcacccgatg agcgtttttg
     1021 attaactgtg atcgttttcg agcaaaaacg atcaaacatg ggcgacacgg tttccaaaga
     1081 actggccgag gccagatggc gcaaggtgcg cacgttgcta cgtgagcgag ggatcctgcg
     1141 cgtggaggag ttggccgctg agttggacgt gtcggccgcc accatccgcc gggaccttgc
     1201 cgcgctggag gcggccggtg ggctgcgtcg tatccacggt ggcgccatcg cgcccgacgg
     1261 gcgggacgag gagccgttgt ttgacgacaa gaccgcgatg tcggccgctg aaaaggggcg
     1321 gatcgccgaa gcggcgttgc gtctgatccg gccgcgcgac atcgtgtatc tcgacggtgg
     1381 cagcacgatt ctggcactgg cgcgcctgct gatccaccac acccaaatca ccgttgtcac
     1441 caattctctg cgcgtagccc aggtgttcag cagcggcggt ccgcgcatga tcctcaccgg
     1501 cggcgaatgc cgcctgatca gtcaaacctt cgtcggtccg ttgacccggt cgatcctggg
     1561 gcaactgcat ctcgatatcg ctttcatggg caccatcggc gtctcggcgg agaccggcat
     1621 gaccacgacc gatcccgccg aagcctacac caaagagcag gccatggcgc gggcggcgcg
     1681 ggtcgtgctg ttggcggaca gttcgaaatt cggcaaaacg agtttcgtgc gttttggtgc
     1741 cctctccgcg ctttccgccc tgatctcaga taaacgcatg ccggccgcgc aacgcaaggc
     1801 gttccagcgt gccggtgtcg aaaccgtact cgtataaccc ccaacacgaa ggcacatcat
     1861 ggcaaccaac aactacatca gtacacggaa acccggtttc aaaccccaga agtacgacct
     1921 cggaaaaatc ccggcctacc agtacaaagg tacgctcaaa gacgaaatcg ccgccgggaa
     1981 catcacggcg gcgcaggcgg tcgatgtgct ggaggacatg ctgacgatcc gcgagttcga
     2041 ggagatgatc gtcaagctgc g
//
LOCUS       DAOSLE010000247         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_47102_length_2054_cov_10.753377, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000247 DAOSLE010000000
VERSION     DAOSLE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_2054_cov_10.753377"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="PK576_10715"
     CDS             <1..1953
                     /locus_tag="PK576_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE62098.1"
                     /translation="PLASFAARGVWLEAEGFEQTGGWLIEQQSFGQVGSAYLMAHGMG
                     IPVDDAITTCTVPRAGKWTVWVRTRDWTAPWKRGTPAGRFQIVVNGTPLPETLGTNGE
                     AWGWQKAGTLPLDKGSAEIALRDLTGFNGRCDALYLTDQPGRVPANDPAALAELRQKT
                     LGTKRKDDPTVYDLAIAGGGISGICTAVAAIRSGAKVILIQDRSVLGGNNSSEIRVPM
                     GGILNKPPYPRLGQVVKEIEPITGGPGPYPPEYYEDARKKHVFETCPRHLYRLMLNTR
                     VIDIEKTGSAKISAFLTRNIRTGEETRIRAKFFADCTGDAVIARMAGASVMYGRESRA
                     TFGESLAPIKGDRQVMGMSIVWYCTRTPSPSAFPAIDWGHELDENTVYYVRSGDWEWE
                     TGQYRDQADETEYIRDYGLMTIFANWSYLKNHAARKAEWANERLAWISPLGGKRESYR
                     VVGDMVLTQNDIEQRVIYPDATASATWNLDLHFPDPIHEAKFKEPFRSCAYHRNITKP
                     YPVPFRCLYSKDIENLFLAGRHISVSHVAFASVRVMRTLGALGEVTGMAAALCAKHNI
                     SPRELHATRLDDLKEMMKRGVPPPPTYHPGGFGSPYEGYHFKDTGHLGVYPNPSPRLN
                     EPSVKARIEALGVQHVETEKQRPSGY"
BASE COUNT          467 a          625 c          585 g          377 t
ORIGIN      
        1 cctttggcga gtttcgccgc gcggggagtc tggctcgaag ccgaagggtt cgagcagacc
       61 ggcggatggc tgattgaaca gcagtcattc ggacaggtcg gctcggccta ccttatggcg
      121 cacggcatgg gaatcccggt tgacgacgcc ataacgactt gcacagtccc gcgtgcgggc
      181 aaatggacgg tctgggtgcg tacgcgcgac tggaccgcac cgtggaaacg cggcacgccc
      241 gccggccgtt ttcagatcgt ggtgaacggg acaccgctgc cggaaacgct gggcacgaac
      301 ggcgaagcgt ggggctggca gaaggctggc acgctcccgc ttgacaaagg ttccgctgaa
      361 atcgcgctgc gtgacctgac cggttttaac ggccgttgcg acgccctcta cctgaccgac
      421 cagcccggca gggttccggc aaacgatccc gccgcgttag cggagctacg gcaaaagacc
      481 ttgggcacca aaaggaaaga tgatccgacc gtttatgatc tcgcgatcgc gggcggcggc
      541 atttccggca tctgtacagc cgttgccgcg atacgatctg gggcaaaagt gatcctgatt
      601 caagatcgtt ccgtcttggg cggcaacaac agttcagaaa tccgcgtacc gatgggagga
      661 attctaaata agccccccta cccgcgcctc ggtcaggttg tcaaagagat cgaaccgatc
      721 acaggcgggc cggggccgta cccgcctgaa tattacgagg acgcccgcaa gaaacatgtg
      781 ttcgagacct gcccgcgtca cctctaccgc ctcatgctca acacgcgcgt catcgacatc
      841 gaaaaaaccg gctccgccaa aatttcagca tttctcacgc ggaacatccg caccggagag
      901 gaaacgcgca tccgtgccaa gtttttcgcc gattgcacgg gcgacgccgt aatcgcgcgc
      961 atggcaggcg catccgtcat gtatggccgt gaatcacggg caacattcgg cgagtccctc
     1021 gcgccgataa aaggcgaccg acaggtcatg ggtatgtcga tcgtctggta ttgcacacgc
     1081 acgccatctc catccgcctt cccggcgatc gactgggggc atgaacttga cgagaacacg
     1141 gtctattatg tacgcagcgg cgactgggaa tgggagaccg gccaataccg agaccaagct
     1201 gatgagaccg aatacatccg cgattacgga ctgatgacca ttttcgcgaa ctggtcctat
     1261 ctcaagaacc acgccgcgcg caaggccgag tgggccaatg aacgtctcgc ctggatctcg
     1321 ccgctcggcg ggaaacggga gagctaccgt gtagtcggcg acatggtcct gacgcagaat
     1381 gacattgagc aacgggtgat ctatcccgac gccacggcgt cggccacatg gaatctggat
     1441 ctgcacttcc ccgaccccat tcacgaagcc aagtttaagg agcccttccg ctcgtgcgcc
     1501 tatcaccgaa acatcaccaa gccctatcct gttccattcc ggtgccttta ctcaaaagac
     1561 attgagaacc ttttcctcgc cggacgacac atcagcgtat cgcacgttgc attcgcctcg
     1621 gtccgcgtga tgcgcacgct gggcgcactg ggcgaggtga ccggcatggc ggcggctctg
     1681 tgcgcgaaac acaatatctc accccgagaa cttcacgcca cccggcttga tgacctcaag
     1741 gaaatgatga aacgtggcgt gccccctcca cccacttatc atccaggcgg cttcggatcg
     1801 ccatacgaag gttatcactt caaagatacg ggacatcttg gcgtctatcc gaaccccagc
     1861 ccgcgcttga atgaacctag cgtcaaagca cgtattgaag cactgggcgt gcagcatgtt
     1921 gaaacagaga agcaacgtcc ttcgggctat tgaaagaact cgctgaatga ccaaaaaacg
     1981 caatcggaaa cagcagattc ttgagctact gatggagcgg ccggaactct ctgtcagtga
     2041 gctgagtcgc cact
//
LOCUS       DAOSLE010000248         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_47996_length_2026_cov_6.726027, whole genome shotgun sequence.
ACCESSION   DAOSLE010000248 DAOSLE010000000
VERSION     DAOSLE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47996_length_2026_cov_6.726027"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1155
                     /locus_tag="PK576_10720"
     CDS             163..1155
                     /locus_tag="PK576_10720"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE62099.1"
                     /translation="MKRIGWIAGVVVCAALCGLADLTTEKKRSAVLLDGVAAYVNTEM
                     ITIADVMGEVRRSPWADTAPELREKRLRELYTATLNALIDRKLILAAARKSKMELQSW
                     AVDNRVREIVANNFDGDQTKLHALLSDRKITFEEWKKNIEEDLLITAMRYQQVEKRVT
                     PTPTEIRAEYEANKGRYQTETAMAVSMIILDPPEKEGDESVAVRAEKIEAALKAGTSF
                     ASLARTYSKDAKAADGGSWGKVNPVDVFRKEIVDALDKLKPGETAPVVVLDGYGYIVR
                     KDEQQDARALTFEEAAPYVESHLRMRQAEKLYKEWTDRLRAESYIKVFELPTSK"
     gene            1160..2017
                     /gene="rsmA"
                     /locus_tag="PK576_10725"
     CDS             1160..2017
                     /gene="rsmA"
                     /locus_tag="PK576_10725"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOE62100.1"
                     /translation="MNLTSPSQVKAWCIANDFHPNKTLGQNFLIDRNTLKATLDAAGV
                     SSGCRVLEIGPGLGVLTEGLLSRGARVTAVEKDTRLASRLAEALGDPPELRVLAADAL
                     EVAWDALLAEGFDACVSNLPYSVGTRILLDLAPHPLAPPVFVVLVQSEVAERFAAPPG
                     RAGRSQAGVWLQLDYTVTPVRTVKASCFWPRPAVASALVRLDRQPCALTPTERVWFHD
                     LTRYAFMHRRKQLGGLLRKAPAPLGREEGALAVLFHSAGVDPLARAEQLTNTQWQSLA
                     RAFAEGVRG"
BASE COUNT          377 a          595 c          684 g          370 t
ORIGIN      
        1 atcctgtcca aattctcccc acctacgacg ccgacggcaa catgcgttgg gacggttctg
       61 aaggatacgt tggttttacc gtggcattcc gtccggcgct aggttacact atcttccgat
      121 tacgcggccg tcgtgtcgcg gggcgacgaa ggagaaaaac gaatgaagcg tatcggatgg
      181 attgcaggcg tagtggtttg cgcggccctt tgcgggttgg cagacctgac cacggagaaa
      241 aagcgttcgg ccgtcctgtt ggacggggtg gccgcctacg tgaacacgga gatgatcacg
      301 atcgctgatg tgatggggga ggtgcggcgc agcccgtggg ccgacaccgc gccggaattg
      361 cgcgaaaagc ggttgcgcga actgtacacc gcgacgctca acgcgctgat cgaccgtaag
      421 ctgatcctcg ctgcggcccg gaaatccaag atggagttgc agtcgtgggc cgtggacaac
      481 cgcgtccgcg agatcgtggc caacaatttt gacggtgacc agaccaaact gcatgccctg
      541 ctctcggacc gcaagatcac cttcgaggag tggaagaaga acatcgagga ggatctgctg
      601 atcaccgcca tgcggtatca gcaggtcgag aagcgcgtga ctccgactcc gaccgagatc
      661 cgggccgaat atgaagccaa caaagggcgt taccagaccg aaaccgcgat ggccgtgagc
      721 atgatcattc tcgacccgcc tgaaaaggag ggggatgaga gtgtggctgt ccgcgctgag
      781 aaaatcgaag ccgcgctcaa agccggcacc tcctttgcct cgctggcccg cacctactcc
      841 aaggacgcca aggcggccga cggcggttcg tggggcaagg tgaaccctgt ggatgtcttc
      901 cgcaaggaga tcgtcgatgc gctggacaaa ctcaagcccg gcgagaccgc gccggtggtg
      961 gtgctggatg gatacggtta catcgttcgc aaggatgagc agcaagacgc gcgcgcgctg
     1021 acttttgagg aggcggcgcc gtacgtcgag agccacctgc gcatgcggca ggcggagaag
     1081 ctctataaag agtggacgga tcggctgcgc gcggagtcgt acatcaaagt gtttgagttg
     1141 ccgacctcca agtgatgttg tgaacctcac gtcgccatcg caggtcaaag cctggtgcat
     1201 cgcaaacgac ttccatccca acaaaacgct gggacagaat tttctgatcg accgcaacac
     1261 ccttaaggcc accttggatg cggcgggcgt gtcatccggt tgccgcgtgc tggagatcgg
     1321 tccggggctg ggggtgctca ccgaggggct gctctctcgc ggggcgcggg tcacggcggt
     1381 ggagaaggac acgcggctgg cctcgcggct ggccgaagcg cttggcgatc cgccggaatt
     1441 gcgcgttctg gcggccgacg cgctggaagt ggcgtgggac gcgctgctgg cggaggggtt
     1501 cgacgcatgc gtctccaacc tgccttattc agtgggcaca cgcattcttc tcgatctggc
     1561 cccgcatccg ttggcgccgc cggtttttgt ggtgctggtg cagtccgagg tggccgagcg
     1621 tttcgccgcg ccgccgggca gggctgggcg cagtcaggcg ggcgtctggc tccagcttga
     1681 ttacacggtc acaccggtgc gcacggtcaa ggcttcctgt ttctggccgc ggccagcggt
     1741 cgcttccgcc cttgtgcggc ttgaccgtca accctgcgcg ctcactccaa cggaacgtgt
     1801 ctggttccac gacctcacgc ggtatgcctt tatgcaccgc cgtaaacagt tggggggcct
     1861 gctgcgcaag gcgcccgcgc cgcttggtcg agaggaaggc gcactcgccg tgttgttcca
     1921 cagcgctggc gtggatccgc tcgcccgtgc cgaacagctc accaacaccc agtggcaaag
     1981 cctcgcccgc gcgttcgcag agggggttag ggggtgaggg ggttag
//
LOCUS       DAOSLE010000249         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_49291_length_1986_cov_5.547385, whole genome shotgun sequence.
ACCESSION   DAOSLE010000249 DAOSLE010000000
VERSION     DAOSLE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49291_length_1986_cov_5.547385"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1463
                     /locus_tag="PK576_10730"
     CDS             168..1463
                     /locus_tag="PK576_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOE62101.1"
                     /translation="MADQDRERLLAGFADHLLRNRLADEKHGRYMVGWVRRFLGFPPP
                     VPNATAEECLQEYLRALEREQAQDWQVEQARMAVTAWLAWRKGQAAAPPQTPKLRLAA
                     DRTVDPARALEAVTETMRVRHYSYRTEQTYLDWARRFFQYLVSTGNVSDGRPVLTEET
                     FQSFISHLATRLRVAAGTQNQAFAAVLFLYRDVLGLEVGRLEDTVRAKRGHRLPTVLS
                     TEEVQKLFDKMEGTPRLMAELIYGGGLRVMECCRMRVKDVDFGMNQLMVRGGKGDKDR
                     STLLPERLKPALRQHLERVRGLFEQDRAAKVAGVHMPDALERKLPHASTEWGWFWLFP
                     SRSLSVDPRTNVVRRHHASDVSVQQAVAQAVAKAGIAKRVTPHTLRHSFATHLLVNGV
                     DIRQVQELLGHANVETTMIYTHVAKGLRAPPKSPLDLLG"
BASE COUNT          392 a          521 c          683 g          390 t
ORIGIN      
        1 cggttttcgg gttgctgagg ggccgtatcg ggttgtcttt tcatggatat ggctgtgtta
       61 ggacaagttg tcctaatcct tttgcgaacg gtgaaatctg cgcaacagat tgtaaggcag
      121 caggttgaaa cttggctttt tgcggaaggg ccggaagggg ggcgggtatg gcggatcagg
      181 atcgggaaag gctcttggcg ggatttgcgg atcacctgct gcggaaccgg ctggctgacg
      241 agaaacacgg ccgctacatg gtcgggtggg tccggcgctt tctgggcttt ccgccgcctg
      301 tgccgaacgc gacggcggag gagtgcctgc aggagtatct gcgggcgctg gaacgggaac
      361 aggctcagga ctggcaggtg gagcaggcgc ggatggccgt gacggcttgg ctcgcctggc
      421 ggaagggaca ggcggcggca ccgcctcaga cgcccaagct caggctggcg gcggatcgca
      481 cggtcgatcc ggcccgtgcg ctggaggctg tgacagagac gatgcgggtc cggcactatt
      541 cctaccgcac ggagcagact tatctggact gggcgcgcag gttcttccaa taccttgttt
      601 cgaccgggaa cgtgtccgac ggtcgccccg tgctgaccga ggagacgttc cagtccttta
      661 tctcgcattt ggcgacgcgc ctgcgcgtgg cggcgggcac gcagaaccag gctttcgccg
      721 cggttctgtt cctctaccgc gatgtgctgg gcttggaggt cggccggctg gaggacaccg
      781 tgcgggcgaa gcgcggacac cggctgccga ccgttttgtc aacagaggag gttcaaaagc
      841 ttttcgataa gatggaaggc acgccgcggc tgatggcgga gctgatctac ggcggcgggc
      901 tgcgcgtgat ggagtgctgc aggatgcgcg tcaaggatgt ggacttcggc atgaaccagc
      961 tcatggtgcg gggcggcaag ggggacaagg accggtcgac gctgctgccc gagcgtctca
     1021 aaccggcact cagacagcat ctggagcgtg tgcgtgggct ctttgaacag gatcgcgcgg
     1081 ctaaggtggc tggggtgcat atgcccgatg ctcttgagcg gaaactgccg cacgcgagca
     1141 cggagtgggg gtggttctgg ctcttcccca gccggagcct gtcggtggat ccgcgcacga
     1201 acgtggtcag gcggcatcac gcttcggacg tgtcggtgca gcaggcggtc gcgcaggccg
     1261 tggccaaagc gggcatcgcc aagcgcgtga ccccgcacac gctgcggcac agctttgcga
     1321 cgcacctgct ggtgaatggc gttgacatcc ggcaggtgca ggagttgctc ggacatgcga
     1381 acgtggaaac gacgatgatc tacacgcatg tggcgaaggg tttgcgcgca ccgccgaaaa
     1441 gccctctcga tctgctggga tgatggtgcc tccaacgccg agaattgcgc ctcggcttcg
     1501 agcgggaata ggggtcgtag tttgacatta gacattcaag ggttgttcag cgttttcgcg
     1561 gtgtggtcat gtgttgccgt ggcaaggcaa ctgaggcacc tccgaaggga tggtggtgcg
     1621 gttgaaaacc gacaacgcct tgcaccggtc gaccgtgtac gtatcaaggc tactggggaa
     1681 acgaaaagtc taatgtcaaa ctacgacccc gacagacgcc ctactgggga aacgaaaagt
     1741 ctaatgtcaa actacgaccc cgacagacgc caacagacgt atttaactca ggacactgaa
     1801 cgtcgaactc tgaactctga aggtgaaaac tccttgccta taacttacag cgatcaaggt
     1861 tatcttcatt taagtgccag tgtgaagcgt gggcggcggg ctaaccaatt acccgagata
     1921 gaagttgaac gttgagcgct gggcgttctt ttgttacggt accccaaaca cttttcacag
     1981 gagaag
//
LOCUS       DAOSLE010000250         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_49720_length_1973_cov_6.425443, whole genome shotgun sequence.
ACCESSION   DAOSLE010000250 DAOSLE010000000
VERSION     DAOSLE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49720_length_1973_cov_6.425443"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1835
                     /locus_tag="PK576_10735"
     CDS             126..1835
                     /locus_tag="PK576_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE62102.1"
                     /translation="MIRDRTAWFLTVLLTGVFSVLLIWPVARVVAGGFRFEGSWTLRY
                     ITGVFANPIYTEGLLNSLRIAIGATALACVLAVPLAWLGHTFSFRGQRLLSALLLVPM
                     VLPPFVGAIGLMQLFSPYGTLNALLGCGPVDWFGQARYAGVILLQALAFYPIIYLNVS
                     VALANTDPALDEAAQNLGAGLLTRVRRITLPLIMPGLFAGCTLVFIACFTELGTPLIL
                     NVSRCAAVQVYDELKEMSASPFPFALVTVVLVCSVLLYGLSRRLFGEHALTTPMRATL
                     AHQPRPIRGLQAALVALPFVLVILLALLPHAGVVLTSITVPGAWYRTTLPTAFTAAHY
                     AEALGHDMTLSAIRNSLLFSLLAVLVNLVLGVTAAWVIVRSRIRARRFLDSVVMLPLA
                     VPGLVMAFGFIALSSSLCNAPGVRAFPQVAALLDIRVNPSLFLVIAYAVRRLPFMVRA
                     AVAGLQQTSVELEEAAANLGATPCCTLRRVTLPLIAANLIAGTLLAFAFSMLEVSDSL
                     MLAQKKAFYPITKTIFELFQLVGIGRYLASALGVWAMFFLTVTIAGCSLLLGRKLGAL
                     FRA"
BASE COUNT          272 a          643 c          603 g          455 t
ORIGIN      
        1 ttttcacact tcgcgcttgc gcgcgcgtgt acccgcagta tcatgctttg tatccgtctg
       61 tagggaggtt gcggccgtgc acaggtgcga cgcgcaaccg atcctgcttc aacggtggag
      121 gatacatgat ccgcgatcgc acagcctggt tcctgaccgt gttgctgacc ggcgtcttca
      181 gcgtactgct gatctggcct gtggcgcgtg tcgtggcggg cggtttccgt ttcgagggat
      241 catggacctt gcggtatatc accggggttt tcgccaaccc tatttacacc gaggggctgc
      301 tcaacagcct gcggatcgcg atcggagcca cggcgctcgc ctgtgtgctg gccgtcccgc
      361 tcgcttggct ggggcacacc ttctcgttcc gcggccagcg gttgctctcc gcccttctgc
      421 tggtccccat ggtgttgcca ccgtttgtgg gggcaatcgg actgatgcaa ctcttcagcc
      481 cgtatggcac gttgaacgcc ctgctggggt gcggaccggt cgactggttc ggacaggcgc
      541 gttatgccgg agtaattctg cttcaggctt tggcgttcta tccgatcatt tacctgaacg
      601 tctccgtggc cctcgcgaac acagacccgg ccttggatga ggcggcgcag aatctcggcg
      661 ccgggctcct cacccgcgtc cggcgcatca cgctgccgct gatcatgccc ggactgttcg
      721 ccggatgcac gctggtcttc atcgcctgtt tcaccgaact cggcaccccg ctgatcctga
      781 acgtctcgcg ttgcgcggcc gtgcaggtat acgacgaact gaaggagatg agcgccagcc
      841 cttttccttt tgcgctggtc acggtggtgc tcgtctgcag tgtgctgctt tacggattga
      901 gccgccgtct attcggcgaa cacgccttga ccacgccgat gcgtgcgacc ctcgcgcatc
      961 agccgcgccc gatccgcggt cttcaggctg cgctggtggc tctgccattc gtgttggtga
     1021 ttctgctggc gcttctgccg catgcgggcg tggtgctcac gagcatcact gtaccgggtg
     1081 cctggtaccg cacaaccctc ccgacagcct tcaccgccgc ccattacgcg gaggcgctcg
     1141 gtcatgacat gacgctgtcc gcgatccgca acagcctgct cttctccctc ctcgcggttc
     1201 tggtcaacct tgttctgggc gttaccgcgg cctgggtcat tgtgcggtcg cgcatccgcg
     1261 cgcggagatt tctggacagc gtggtcatgt tgccgctcgc ggtcccgggt ctggtcatgg
     1321 ccttcgggtt tatcgcgttg agttcctcac tgtgtaacgc gccgggcgtg cgtgccttcc
     1381 cgcaggtggc cgcgttgctg gacatccgcg tcaacccttc gctttttctg gtgatcgcct
     1441 atgcggtgcg ccgcctaccc ttcatggtcc gggccgcagt ggcgggcctg cagcaaacct
     1501 ctgtcgagtt ggaggaggcc gcggccaatc tcggtgccac accatgttgc acgttgcggc
     1561 gcgtcaccct tccgctgatt gcggcgaacc tgatcgccgg cacgctgctg gcgttcgcct
     1621 tcagcatgct ggaggtctcc gacagtctga tgctggcgca gaaaaaggcc ttttatccga
     1681 tcaccaagac catcttcgaa ctcttccagc tggtcgggat cggccggtat ctggcctccg
     1741 cgctcggcgt gtgggccatg ttcttcctga ccgtgacgat cgcggggtgc agtctcctgc
     1801 tgggacggaa actcggcgcg ttgttccgtg cttgatcgca cggccgcgct gtgaagattc
     1861 attcatcttc caaagttccg agtcgatcac aaaaaaaagg gggggccgcg gatcgtcccg
     1921 atccgcggcc ccccggaaaa aaactcccgg cggcgtccta ctctcccgca ggc
//
LOCUS       DAOSLE010000251         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_50015_length_1965_cov_7.314660, whole genome shotgun sequence.
ACCESSION   DAOSLE010000251 DAOSLE010000000
VERSION     DAOSLE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50015_length_1965_cov_7.314660"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1965)
                     /locus_tag="PK576_10740"
     CDS             complement(<1..>1965)
                     /locus_tag="PK576_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOE62103.1"
                     /translation="ALPAASRFAPENVYWISRNPGLTMSESHQVPSVPAVTNAWFTDR
                     IERQGTEWLGRISYSTLTNAHIPFTGYRTLSGGGSSYILTNTLTDCASGDWSGAVTVR
                     LLSRYDGEDGGDTHLACLAIGDSTLGDIAWTNEAYQVATFPFASASAPDGTLFLTLTN
                     AATPPPYPLNDFTKFSCLSYAIDYARRYVARDDRLRCTGGSGSLAAVTHFTTNDVLVL
                     DVTDAARPLRVSGCEQLQDAATGLWSVSFACGDATRIYRLASNSKGLLQPAVRGVRDT
                     DWGAPAQATPHVILIPPEAWRSGFRQAVEPLRAFRTSQGIPSRIVDVEALYNAFSDGL
                     VDPEAIRAFCSAMRPNGLTYLLLAGAGAVDFKHHRLSVTDYTSCLIPTLIAGQRMIQT
                     GEGMIVALDGALGDTDGDGRPDVAVGRLPTSQTQQVTTVVGKTIAYETRPPRTKRPVS
                     LVADWENVYPKDYDFSNGIDRLQEPLRRAGRSVMRHVLPDGNSGTAARKTFLFPALQA
                     GAGLFHFFGHSNESSLGDGDRFLSIANIQPGNWRIPPTSVVIACRANRWHGLTPTQLI
                     LPFGLFATDTGFAGCLGATGNMRAREGEELAVALFTPDTGEQTVTLGKVWLRGMRSMA
                     GKIPQERLLCFSLIGDPALRFGVPPYPGTA"
BASE COUNT          386 a          605 c          635 g          339 t
ORIGIN      
        1 ccgcggttcc cggatagggc gggacgccaa agcggagcgc cggatcgccg atcaggctga
       61 aacacagcag gcgttcctgc gggatttttc ccgccatgct ccgcatgccg cgcaaccaca
      121 ctttgcccag cgttaccgtc tgttccccgg tatcgggcgt gaacagcgca accgcgagtt
      181 cctcgccttc tctagcccgc atattcccgg tggcgcctag gcacccggcg aaccccgtgt
      241 ccgttgcgaa aaggccaaac ggcaggatca attgggtcgg agtcagcccg tgccagcggt
      301 tggcccggca tgcgatcacc acgctggttg gcgggatccg ccaattaccg ggctgaatgt
      361 tggcaatgga cagaaatcgg tcaccgtcac ccagactaga ttcgttggag tgcccgaaaa
      421 agtgaaaaag tccggcaccg gcctgcagcg caggaaagag gaaggttttg cgagccgccg
      481 ttccggagtt gccgtcgggc agaacgtgtc tcatgactga tcgcccggcg cgcctcagag
      541 gttcttgcag ccgatcgata ccgttgctga agtcataatc cttgggatag acattctccc
      601 aatccgccac aagcgaaacc gggcgtttgg tgcggggagg ccgggtttca taggcaatgg
      661 ttttgccgac cacggtcgtg acctgctgcg tctgggaagt cggcagccgg ccgaccgcca
      721 catcaggtcg gccatccccg tccgtatcgc ccagtgcgcc gtcgagcgcc acaatcatcc
      781 cctcgccggt ttggatcatc ctctgtccgg cgatcagggt gggaatcagg caagaggtgt
      841 aatccgtgac ggacagccgg tggtgtttga agtccactgc cccggcaccg gcgaggagga
      901 ggtatgtcag cccgttggga cgcatcgcgg aacaaaaagc gcggatcgct tccggatcga
      961 ccagtccgtc gctgaatgcg ttgtaaagcg cctcgacatc cacaatgcgg gagggaatcc
     1021 cctgagacgt gcggaatgcg cgtaaaggtt ccacggcctg ccggaaaccg ctgcgccacg
     1081 cttccggcgg gatcaggatc acatgcggcg tcgcctgcgc gggcgcgccc caatccgtat
     1141 cgcgcacgcc gcgcacggca ggttggagaa gccctttcga gttggaggcg agccggtaga
     1201 tccgggtcgc gtcaccgcac gcgaacgaaa cggaccacag ccccgtggcc gcatcctgaa
     1261 gctgctcgca tccgctcacg cgcaggggcc gcgcggcatc ggtgacatcc aggacgagca
     1321 cgtcattcgt ggtgaagtgg gtgaccgcag cgagggaacc gctcccgccc gtacaccgca
     1381 ggcggtcgtc gcgcgcgaca tagcgtcgtg catagtcgat ggcataggag agacaggaga
     1441 atttggtaaa atcattgaga ggataagggg gcggagtggc cgcattggta agcgtcagga
     1501 agagagttcc gtcaggcgcc gatgcggaag cgaaagggaa ggtggccacc tgataagcct
     1561 cgttcgtcca cgcgatgtcg ccgagcgtgc tgtcgccgat cgcgaggcac gcaagatgcg
     1621 tgtcgccccc gtcctcacca tcgtaccggg agagaaggcg caccgtcacg gcgccgctcc
     1681 agtctccgga cgcgcagtcc gtcagcgtat tggtcaagat atacgaagag cctccccccg
     1741 acaatgtcct atacccggta aacggtatgt gcgcattcgt cagcgtactg tacgatatgc
     1801 gcccgagcca ttcggtgccc tgccgctcga tgcggtccgt gaaccaggcg ttggtgacgg
     1861 ccggaacgga cggcacctga tgactctcgc tcatggtcag gccgggattt cgggagatcc
     1921 agtacacgtt ctcgggtgcg aaacgcgatg ccgcaggcag cgcat
//
LOCUS       DAOSLE010000252         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_50095_length_1963_cov_8.103774, whole genome shotgun sequence.
ACCESSION   DAOSLE010000252 DAOSLE010000000
VERSION     DAOSLE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50095_length_1963_cov_8.103774"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..976)
                     /locus_tag="PK576_10745"
     CDS             complement(68..976)
                     /locus_tag="PK576_10745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE62104.1"
                     /translation="MLGDCLEVMAGLPDGAIDAVICDPPYGTINGAGLDGWCEGDTDW
                     DDALDPAAVFAHCNRVLRMNGALVLFSQEPYTSRLITEAHGNLPFSYRMTWLKDHFAN
                     ALVAKKAPVSYTEDVLVFFKKYDTQAQHPLRQYAKRVLAICGGDLKAINARLGHRRAE
                     HFFYVESTQFALCTEQTYAQLCEVYGLARMEWFKPYAELEEINRRFKRCFNLPEGQKY
                     KSNVLQYRKDYSGHHPTQKPVALMEDLVKTYTNPGDTVLDFTMGSGSTGVACVKTKRN
                     FIGIELDEGYFKIAEQRIRETEPPIF"
     gene            complement(1032..1808)
                     /locus_tag="PK576_10750"
     CDS             complement(1032..1808)
                     /locus_tag="PK576_10750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006621133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE62105.1"
                     /translation="MKEIDDGSVDLTVTSPPYDNLRTYNGTLNDWTQEKWQAIIRELF
                     RVTKQGGVVVWIVGDATINGSETGTSFKQALWAIECGFRLHDTMIYHREGPPLTHNRY
                     EQHFEYMFVWSKGKPSAFNGQRETSIKAGRTRKGFTSRQDGEVLGTRGEHGFVSPTKL
                     RGNVWKFAIGNNGSTSDKIAFKHPAIFPEALARDHILSWSNPGDTVLDPFLGSGTTGK
                     MAILEGRRFIGIEREEKYFDIACKRIEEAQRIMETEPPIF"
BASE COUNT          391 a          614 c          510 g          448 t
ORIGIN      
        1 aaacaccctc ccggtgtggt aaaagctctt ccgcgtctca cggaaggcat aaaaagccgc
       61 ctcccgatca aaaaataggc ggctccgtct ccctgattcg ctgttccgct attttgaaat
      121 acccctcgtc aagttctatc ccgatgaagt tccgtttcgt tttcacgcac gccacgccgg
      181 ttgatccgct gcccatcgtg aagtccagca ccgtgtcgcc ggggttcgtg taggtcttta
      241 ccaagtcctc catcagcgcg acgggcttct gcgtcggatg gtggccgctg tagtccttcc
      301 ggtattgcag gacgttggac ttgtatttct ggccttccgg caggttgaag cagcgcttga
      361 agcggcggtt gatttcctct agctccgcgt agggcttgaa ccattccatg cgcgccaacc
      421 cgtacacctc gcacaactgc gcgtaggtct gttccgtgca aagcgcaaac tgcgtggact
      481 ccacatagaa aaagtgctcc gcgcgccgat gccccagccg cgcattgatc gccttcaggt
      541 cgccaccgca gattgccagc actcgcttgg cgtactgccg caacggatgc tgcgcctgcg
      601 tgtcgtactt cttgaagaaa accagcacgt cctcggtgta ggagacgggc gccttcttcg
      661 ccaccagggc gttagcgaaa tgatccttca gccacgtcat ccggtagcta aacggcaggt
      721 tcccgtgagc ctcggtaatc agtcgggacg tgtacggctc ctgcgagaac agaaccagcg
      781 cgccgttcat ccgcaacacg cggttgcagt gcgcgaacac ggcggctggg tccagcgcat
      841 cgtcccaatc cgtatcacct tcgcaccagc cgtcaagccc cgctccattg atagtcccat
      901 agggcggatc gcagataacg gcatcaatcg ccccatccgg cagccccgcc atcacctcaa
      961 ggcaatcacc caacatcagc cgtatcgttg aacgcctccc ttcgagagga tacaaaaaag
     1021 ccgcctcccg atcaaaaaat aggcggctcc gtttccatga ttcgctgtgc ttcttcgatt
     1081 ctcttgcaag caatatcgaa atacttttcc tcgcgctcta tgccgatgaa acggcggccc
     1141 tcaaggatcg ccatcttgcc cgtcgtgccg ctgccgagaa acgggtcgag taccgtatcg
     1201 ccgggattgc tccagcttag gatgtgatcg cgggcaaggg cttcggggaa tatggcgggg
     1261 tgcttgaaag ctattttgtc ggaagtgctg ccattgttgc cgatggcaaa tttccagaca
     1321 tttccacgta acttcgttgg cgagacaaag ccatgttcgc cgcgagttcc taaaacctcg
     1381 ccgtcttgtc gggaagtgaa gcctttgcgc gtccgaccgg ccttaatgct ggtttctctt
     1441 tgaccattga aggctgatgg tttccccttg ctccacacaa acatgtattc gaaatgctgt
     1501 tcgtagcggt tatgcgtgag cggcggtccc tcgcggtgat atatcatcgt atcgtgcaat
     1561 cggaacccgc attcaatcgc ccacaacgct tgcttaaacg atgtgcctgt ctcgctgccg
     1621 ttgatcgtgg catcgcccac gatccacacc acaacgccgc cctgcttggt gacgcggaac
     1681 aactcgcgga tgattgcctg ccatttctcc tgcgtccaat cgttcagtgt gccgttgtag
     1741 gtccgcaggt tgtcgtatgg gggagacgtg acggtcaagt ccaccgagcc gtcgtcaatc
     1801 tccttcatgc gctctataca atctcccaac atcagccgta tcgttgcccg cctcccttcg
     1861 tgagaataaa aaagccaccc ctggggaggc ggccacaacc cggcgtcaag gtagcgcttg
     1921 cccggcgcgg gttaaacttg cttcctcaac tgttccagcg tca
//
LOCUS       DAOSLE010000253         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_50965_length_1939_cov_10.214437, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000253 DAOSLE010000000
VERSION     DAOSLE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50965_length_1939_cov_10.214437"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /gene="prs"
                     /locus_tag="PK576_10755"
     CDS             complement(<1..436)
                     /gene="prs"
                     /locus_tag="PK576_10755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOE62106.1"
                     /translation="MIVFSGTANLPLAEKIMAYLGGRLGDINIRRFPDGEIHVKIVDN
                     VRGRDVFIVQPICTQPNEMLMELLIMIDAARRGSAARITAVLPYYGYARQDRKDQPRV
                     PITAKLVANLLVAAGANRMLTMDLHAQQIQGFFDIPVDHLHAA"
     gene            complement(466..539)
                     /locus_tag="PK576_10760"
     tRNA            complement(466..539)
                     /locus_tag="PK576_10760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Gln,seq:ttg)
     gene            complement(525..1430)
                     /gene="ispE"
                     /locus_tag="PK576_10765"
     CDS             complement(525..1430)
                     /gene="ispE"
                     /locus_tag="PK576_10765"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOE62107.1"
                     /translation="MVRAGSVITLEAYAKINLTLEVLGKRPDGYHELRSIVMPVTLSD
                     TIALTSTYAQTAMTITAGPGVDLTKIGAMEHNLAVRAARLMQLRYQVNAGARIHIHKR
                     IPIGGGLGGGSADAAGVIVGLDRLWELGRPRAELMALGAELGSDIPALIHGGAVLMEG
                     RGERVRGLFEGTPTPTCQGFWLVIANPGIMCSTPEVFRKWRAGLTASPLLLHNMTSSI
                     RTSDVSAAALALHNGLQPAVYESYPGVAQTAARLQEAGCLGVLLSGSGASVFGLARDE
                     AHGREISRRLGAGVWHVVAQTCPVV"
     gene            1639..>1939
                     /locus_tag="PK576_10770"
     CDS             1639..>1939
                     /locus_tag="PK576_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62108.1"
                     /translation="MNRFTGIVVSIGLAAGFAVAERTAPAVFHVDSEAGNDSAEGTRP
                     DAAWRTLDKVNTATLIPGDRVLFKCGGLWRGQLMPKSGTPDARILYGAYGEGAKPI"
BASE COUNT          378 a          597 c          588 g          376 t
ORIGIN      
        1 gcgcggcgtg caggtggtcg acgggaatat caaaaaatcc ttggatttgc tgggcatgca
       61 ggtccatggt cagcatgcgg ttggcaccgg cggccaccaa caggttggcc acgagtttcg
      121 cggtgatcgg cacgcgcggc tggtctttac ggtcttggcg cgcatagccg tagtagggga
      181 gcactgctgt gatgcgcgcc gccgaacccc ggcgcgcggc gtcgatcata atcagcagtt
      241 ccatcagcat ctcgttgggc tgcgtgcaga tcggctgaac gatgaaaacg tccctgccgc
      301 gcacattgtc gacgatcttg acgtgaattt caccgtccgg gaaacgacgg atgttgatgt
      361 cgcccagtcg gccgccgagg tacgccatta tcttctcggc cagcggtagg ttcgcggttc
      421 ctgaaaaaac aatcatgctc gcacacactt tcctaaacac aaaactggtt gcccggggtg
      481 gactcgaacc acctccgaga gagtcaaagt ctcttgtgct gccattacac caccgggcag
      541 gtttgtgcca caacgtgcca cacgccggca cccagtcgcc ggctgatctc ccgtccgtgt
      601 gcttcgtctc tcgccaatcc gaatactgac gccccgctgc cggaaagcaa aacacccaaa
      661 cagcccgcct cttggagccg ggcggccgtc tgggcaacac cggggtaact ttcgtagacc
      721 gccggttgca aaccgttatg aagcgccagc gccgcagccg agacgtcgct ggtacggata
      781 gaagaagtca tattatggag aaggaggggg cttgcagtca agcctgctct ccattttctg
      841 aaaacctcgg gcgtggagca catgatgccg gggttggcga tgaccagcca gaaaccttgg
      901 caggtcggcg tcggcgtgcc ctcgaagagc ccccgcacgc gttcgccgcg tccctccatc
      961 agcacggcgc cgccgtggat caacgcggga atgtcgctgc ccagttccgc gcccagcgcc
     1021 atcagctccg cgcgcgggcg tcccaattcc cagaggcggt ccaaccccac gatcactccg
     1081 gcggcatccg cgctgccgcc tcccagtcca ccgccgatcg ggatgcgctt gtggatgtgg
     1141 atgcgcgcgc cggcgttgac ctgatagcgg agctgcatca gacgcgcggc acgcaccgcc
     1201 agattatgct ccatcgcgcc gatcttggtg aggtccactc ccggcccggc ggtgatcgtc
     1261 atcgcggtct gcgcgtaggt ggaggtgagc gcgatggtat cggacagcgt gacgggcatc
     1321 acgatactgc gcagttcgtg gtacccgtcc ggccgcttgc ccaagacctc cagcgtcagg
     1381 ttgatcttgg catacgcttc cagtgtgatg acagaccctg ctctgaccat ttgggcgcgt
     1441 attctgccat aacgtgccgt gcgcttcaag agagaggtga tatattgagg taatatatga
     1501 gggaactaaa gcactaacga actaacacac taacgaacta acgcacgtcc ttgattccta
     1561 ccccgttgtg ccctacactg aaacctatgt cgcggcggtc tgtcagagcc gcctgttaaa
     1621 aaaaggggag atgtttgcgt gaatcgtttt acaggaattg tcgtgtccat cgggcttgcc
     1681 gccggttttg cagttgcgga gcgcaccgcg ccagccgttt ttcatgtcga tagcgaagcg
     1741 gggaatgaca gcgcggaggg cacgcggccc gatgccgcgt ggcgtacgct cgacaaggtt
     1801 aacacggcca cgctcattcc cggcgaccgg gtgctcttca aatgcggcgg actctggcgc
     1861 gggcagctca tgccgaagag cggtacgccc gatgcgcgca ttctctatgg cgcttacggt
     1921 gaaggcgcca aaccgatcc
//
LOCUS       DAOSLE010000254         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_51047_length_1937_cov_5.219979, whole genome shotgun sequence.
ACCESSION   DAOSLE010000254 DAOSLE010000000
VERSION     DAOSLE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51047_length_1937_cov_5.219979"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="PK576_10775"
     CDS             <1..1533
                     /locus_tag="PK576_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62109.1"
                     /translation="AYGGPVTLTLDPLPGTLAGLPGATNGPWLCGMSILPARSNTVVF
                     ADGQTPPPPPGNPDSVDGLLLLSPPPSGAVHALTSPPPSAVLQPLRMTAGVTVLGNGG
                     WYCLRESSLCSWPDYDMIGCDPVSMAMIGVTGDTPLLSREAALQIFWARHPCVQCTVT
                     QPVVNAVYPFLYARVIFSFRPCEAAGEVRGAEQNLPWHTPEHGHFSLCSGIGCVCEGS
                     GYWTIGFSHSAVNTRNLSRLGTGNGDADATEHCLGVVWSAGGKVDLFALLHDSHQPHK
                     DDLSFTSDNLAVNNNGELVFGDNPKELEPQISLVKLRYKPTPKINVVFDKLWVVVNRS
                     DTKTKFDNWYAQNADMSWTATLPRPFASIALATNATGEVTGPIDPEPGWFGGQWHAPE
                     AKNTFLHHNAKYEMRSKPTSDGHGHQAMYDAHGYLITSPIAAGTADMYAPFYSIGAPR
                     LSTKHLEQDVQPFIRALQLDGNPVLPRDIYRNLNRPCIYVGLNTDTYIKKRPVLPTGV
                     QP"
     gene            1530..>1937
                     /locus_tag="PK576_10780"
     CDS             1530..>1937
                     /locus_tag="PK576_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62110.1"
                     /translation="MNIKKTVSLSVLFVSVLLQVDLCYGQSNVVYYGANSNAISLAFA
                     DTNLSESAKSAITADLRVCLSEWGKGELRMRIKGDSVGYIDNPTHCPHYPEDIEFPEN
                     VVPSGAAGLALHIPKKLSDAYTNAFAFAAANSNI"
BASE COUNT          396 a          666 c          525 g          350 t
ORIGIN      
        1 gcctacggcg ggccggtcac gctcacgctc gaccccctgc ccggaacgct cgcgggactt
       61 cccggcgcga ccaacggccc gtggctctgc gggatgtcga tccttccggc gcgctccaac
      121 accgtcgtct tcgcggacgg ccagacgccg ccgcccccgc ccggcaaccc ggacagcgtg
      181 gacgggctcc tcctgctctc gccgccgccc tccggcgcgg tccacgccct gacctcgccg
      241 cccccgtccg ccgtgctcca gcccctgcgc atgacggccg gcgtgaccgt cctcggcaac
      301 ggcggctggt actgcctgcg cgaaagctcc ttgtgctcct ggcccgatta cgacatgatc
      361 ggctgcgacc cggtcagcat ggcgatgatc ggcgtcacgg gcgacacccc gcttctttcc
      421 cgtgaagccg cgctgcagat cttctgggcg cggcacccct gcgtccagtg cacggtgacc
      481 cagcccgtgg tgaacgcggt ctacccgttc ctgtacgcca gggtcatctt ctccttccgg
      541 ccctgcgagg cggcaggcga ggtccgcggc gcggaacaga atctgccgtg gcacacgccg
      601 gagcacggcc acttctccct ttgcagcggg atcggctgcg tctgcgaggg ttcggggtat
      661 tggaccatcg gcttcagcca ctccgccgtc aacacccgca acctctcccg cctcgggacc
      721 ggcaatggcg acgcggacgc caccgagcac tgcctcggcg tggtctggag cgcaggcgga
      781 aaggtcgacc tcttcgccct gctccacgac agccaccagc cccataaaga cgacctgagc
      841 ttcacctccg acaacctggc ggtcaataac aacggcgaac tggtcttcgg cgacaatccc
      901 aaggagttag aaccccaaat cagcctggtc aaactacgct acaagcccac cccgaaaata
      961 aacgttgttt tcgacaagct ctgggtggtc gtcaacaggt ctgacacaaa gacgaaattc
     1021 gataactggt atgcgcaaaa cgccgacatg agttggacgg caaccctgcc gcgcccgttc
     1081 gccagcatcg ccctcgcgac gaacgctacg ggagaggtca ccggccccat cgacccggaa
     1141 cccggttggt ttggcggaca atggcatgct cccgaagcga aaaacacctt cctgcatcat
     1201 aacgcgaagt acgagatgcg ctccaaaccg acctccgacg gacatggtca ccaagcgatg
     1261 tacgatgctc acggttacct cattaccagt cccatcgcgg cggggacggc tgacatgtat
     1321 gccccttttt actcaattgg cgcacctcgg ttgagtacga agcatttgga acaagatgtc
     1381 caaccgttca tccgcgcctt gcaactcgat ggcaatccgg tgctccccag agacatctac
     1441 cgcaatctca accgcccatg tatctacgta gggctaaaca cggacacgta catcaaaaaa
     1501 cgccccgtct tacccacagg agtacagcca tgaacatcaa aaaaacagtt agtctttccg
     1561 tcctcttcgt gtccgtgtta ttgcaagttg atctgtgtta tggacagagc aatgtcgttt
     1621 actacggagc gaactcaaac gcgatcagcc tcgcgtttgc ggacaccaat ctttccgagt
     1681 cggcgaaatc ggcgatcacg gcggatttgc gggtgtgctt gagcgagtgg gggaaggggg
     1741 aattgcgcat gcggatcaag ggtgattcag ttggatacat cgataacccc acccactgtc
     1801 cgcattatcc cgaagacata gagttccccg aaaatgtcgt gccaagcggc gcagcaggtc
     1861 tggctttaca tatccccaaa aaactcagcg atgcctacac caacgccttc gcgttcgccg
     1921 ccgccaactc caacatc
//
LOCUS       DAOSLE010000255         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_54491_length_1849_cov_3.900223, whole genome shotgun sequence.
ACCESSION   DAOSLE010000255 DAOSLE010000000
VERSION     DAOSLE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54491_length_1849_cov_3.900223"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1456)
                     /locus_tag="PK576_10785"
     CDS             complement(254..1456)
                     /locus_tag="PK576_10785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOE62111.1"
                     /translation="MKKRMIGNLLVMCGLSVTTLSGETQEPVAEIWGAPVRQKRAEIL
                     WTKVLCKQPGRYIGWPTVCARANGELVAVFSGDRDGHVCPFGKVQMVRSADGGETWSP
                     AVNIGNTPLDDRDAGIVELPGGDLVASWFTSVAYTGSIRDRAKLNPGSPRFYWWLHDE
                     KIPAQTKRDGLGYFTIRSSDGGKSWETPVRSPGTAPHGPILLKDGRLLYVGITYSGVF
                     GVFSGAAYEVSVAESRDGARSWQRISAIPLPTEVKIGDFHEPHAVETADGRIVAQIRY
                     HGKGQGAATRLWQSESADGGATWAVARATDLAGLPPHLIRLRDGKLVSVYGRRFAPLG
                     EYACISDDHGRTWDVANEIKLAGHFNGDLGYPASTELPDGSILTVYYQADKQGEQTCL
                     MGTKWRVR"
     gene            complement(1608..>1849)
                     /locus_tag="PK576_10790"
     CDS             complement(1608..>1849)
                     /locus_tag="PK576_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62112.1"
                     /translation="TNMFLKVTSGGTVNLDFEGEVEVGHLVINGFEWPGRGMRYGSSQ
                     SLGEVDRVMDGTFTGTGVLKVVGPRGLQGTLIKLR"
BASE COUNT          352 a          623 c          499 g          375 t
ORIGIN      
        1 cgcccatgcc acgaggtttt ttacaagagt ctcctctaat cagtctcaaa ttctagcacc
       61 attcgccgcg tgtgtcaggg ggggctcgtc actggttagt gacaggggtt atacgagtcg
      121 ggagcaaaaa aatgcgcaac ttgtccatct gtggataaaa aaaccgagca gtgctcccga
      181 aaaaccttag tgccctttgt gtctccgtgt gagaaacaac ttgtttttat cgcgcgtatt
      241 gcaccgtaag gtactagcgc acccgccact tggttcccat cagacaggtc tgctctcctt
      301 gtttgtcggc ctgataataa accgtcaaaa tactgccgtc cggcagttcg gttgaggccg
      361 gatagcccag gtcgccgttg aaatgtccgg ccaacttgat ctcgttggcc acgtcccatg
      421 tgcgtccgtg gtcgtcgctg atgcaggcat attcgccgag cggggcgaag cgccgtccgt
      481 acaccgacac cagcttgccg tcacgcaggc ggatcaggtg cggcgggaga ccggccaaat
      541 cggtcgcccg cgccacagcc cacgtcgcgc caccgtccgc gctctcggat tgccacagcc
      601 gcgtcgccgc gccctgtccc ttgccgtggt agcgaatctg cgccacgatc cttccgtccg
      661 ccgtctcgac cgcgtgcggt tcatggaagt cgccgatctt cacttccgtc ggcagcggga
      721 ttgcgctgat gcgctgccac gagcgggcac cgtcgcgcga ctcggcgacc gacacctcat
      781 acgccgcccc gctgaacacc ccgaaaaccc cggagtaagt gatcccgaca tagagcagtc
      841 gcccgtcctt gagcaggatc ggcccgtgcg gcgcggtgcc gggcgaacgc accggcgttt
      901 cccagctctt gccgccgtca gacgagcgga tcgtgaagta acccaggccg tcccgcttgg
      961 tctgcgccgg gatcttctcg tcatgcagcc accagtagaa gcgcggcgag ccgggattca
     1021 gcttggcgcg gtcgcggatg ctgcccgtgt aggccactga ggtgaaccat gaggccacca
     1081 gatcgccgcc gggcagttcg acaatacctg catcgcggtc atccagcggt gtgttgccga
     1141 tgttgaccgc tggcgaccac gtctcgccgc catccgcgct acgcaccatc tgtaccttgc
     1201 cgaaaggaca gacgtgcccg tcgcgatcac cggaaaacac cgccacaagc tcgccgttgg
     1261 cgcgggcaca gactgtcggc cagccgatat agcgccccgg ctgtttgcac agtaccttag
     1321 tccacaggat ttcggcacgt ttctgtcgta ccggcgcacc ccagatctct gccaccggct
     1381 cttgcgtttc gcccgacagc gtcgtcacgc tcaaaccgca catcaccaaa agattaccaa
     1441 tcattctctt tttcattgca tccttccctt gccgcgttat gcgcggagtt aggagttaaa
     1501 agttgggagg gatgagcgtc tgctcgtcct tgggcaagta gacgcttgcc ctcccattcc
     1561 tgcactaact aacacaccaa tgaactaatg cactgccccc tcacacctta tcgcaacttg
     1621 atcagcgttc cctgtagccc acgcgggccg accaccttca gcacgccggt tcctgtaaag
     1681 gtcccgtcca tcaccctgtc gacctcgccc aacgattgcg aagagccgta gcgcataccg
     1741 cgtcccggcc actcgaagcc attgatcacc aagtgcccga cctcgacttc gccttcgaag
     1801 tccagattga ccgtgccacc cgacgtcacc ttcaggaaca tgttggtgt
//
LOCUS       DAOSLE010000256         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_55307_length_1828_cov_7.703328, whole genome shotgun sequence.
ACCESSION   DAOSLE010000256 DAOSLE010000000
VERSION     DAOSLE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55307_length_1828_cov_7.703328"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK576_10795"
     CDS             <1..266
                     /locus_tag="PK576_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62113.1"
                     /translation="NRAFNYVIRNNVICHSIDRLMDVGSGLKNPDGSSSMPRLEGNVF
                     IERTGGVFGKITEAPVRDRMCDTNTPAFLETFGPGNRCVWLEK"
     gene            355..771
                     /gene="speD"
                     /locus_tag="PK576_10800"
     CDS             355..771
                     /gene="speD"
                     /locus_tag="PK576_10800"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOE62114.1"
                     /translation="MKKNALGRHILTELYGCPMETLDDLQLITDAMTAAAIETGADIL
                     KTAFHQFSPQGVTGFVMIAESHLAIHTWPEYGYAALDVFTCGSNVDPWIASQYLEKRL
                     RAKSVRTKEVFRGIFHNAPGRRPTDSDRAEYQYVLF"
     gene            758..1612
                     /locus_tag="PK576_10805"
     CDS             758..1612
                     /locus_tag="PK576_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOE62115.1"
                     /translation="MFFSDKEWHADRTSPGVVHLFPLSGTLVDQQTAFQRIEIVENEI
                     FGRMLILDGELQSAAFDEHRYHEALVHPAMVLHPSPRRVLIAGGGEGATAREALRHES
                     VSQLVMADIDGEVVELCRLHLPQWHGNCYDDPRMELYIGDVLRLLEDRSDRYDVIILD
                     LTEPDEDGPAHHLATRQFYQLARDRLSPEGIVAMQAGDFNPVNLRTHTAAYTTLKEVF
                     PHVASYRVFVPSFHTEWGFLLASPHHPVERSTEVRQEIADTIAARKLELRFYNADVHC
                     GMFCSGMR"
BASE COUNT          375 a          476 c          551 g          426 t
ORIGIN      
        1 gcaaccgggc gttcaactac gtgatacgca ataacgtgat ctgccactcg attgaccgtt
       61 tgatggatgt ggggagcggg ctcaaaaacc ccgacgggtc ttcatccatg ccgcgtctgg
      121 aagggaatgt cttcatcgag cggacgggtg gcgtgttcgg taaaatcacg gaggcgcctg
      181 tgcgtgaccg catgtgcgac acaaacaccc ccgcgttcct tgagacgttc ggccccggta
      241 accgatgcgt gtggctggag aagtgatgag tgtaaaagtg ttgctctgct ttttcgtcgc
      301 atttgacatc ttggctttct gtggtatggt gtctgctata aggaggtgtt cggtgtgaaa
      361 aagaatgcgc tgggacgtca tattttgaca gagttgtacg gatgccccat ggagaccctt
      421 gatgacctcc agttgataac cgacgcgatg accgctgccg ccatcgaaac tggtgccgat
      481 attcttaaaa cggccttcca ccagttttca ccgcaaggcg tgaccggttt cgtcatgatc
      541 gcagagtcgc atttagcgat tcatacttgg cctgaatatg gctacgccgc actggatgtc
      601 tttacctgcg ggtccaacgt ggatccttgg attgcgtcgc aataccttga aaagcgccta
      661 cgcgccaaat cagtccggac caaggaggtc ttccgcggta tctttcacaa tgcgccggga
      721 cgcagaccga cagattcgga ccgggcggag tatcagtatg ttcttttctg acaaggagtg
      781 gcacgctgac cgaacaagtc cgggcgtggt tcatctcttc ccgctgtcgg gtacgttggt
      841 cgatcaacag acagctttcc agcggatcga aatcgttgag aacgagattt tcggcaggat
      901 gctgattctg gatggcgagt tgcaatccgc tgcctttgat gaacatcgct accacgaggc
      961 gctggtgcat ccggccatgg ttttacatcc atcgccccgg cgtgtcttga tcgccggcgg
     1021 gggagagggc gcgacggctc gtgaagcgct tcggcacgag agcgtttcgc aactggtcat
     1081 ggcggacatc gatggcgaag tcgtggaact ctgccgcctc catttgccgc agtggcacgg
     1141 gaactgttac gacgatccgc ggatggaact gtacatcggc gatgtgttgc gccttctgga
     1201 agatcgctcg gaccgctacg atgtgattat tctggatctg accgaacccg acgaggacgg
     1261 ccccgcgcat caccttgcca cacgccagtt ttatcaactg gcacgcgacc gattgtcgcc
     1321 cgaggggatc gtggccatgc aggccggtga ttttaatccg gtcaaccttc gcacacacac
     1381 ggcggcgtac acgacgttaa aagaggtctt cccgcatgtc gcgtcgtatc gtgtcttcgt
     1441 gccttcgttc catacagagt gggggttctt gttggcatcg ccgcatcacc ctgtggaacg
     1501 cagcactgag gtccggcaag aaatcgcgga tacgatcgcc gcaagaaaat tagagctgcg
     1561 cttttacaat gctgacgttc attgtggcat gttctgcagc ggcatgcgtt gaccggttaa
     1621 caggcgaaat gatacgatca ctgcgtaacg ttcaaagtga aacggggcgg aatgcagagc
     1681 gtccgtaata aagggggaaa gtcgatgatg cgtgagcggt gtacgtgctt tcgtgtgttt
     1741 gcggcagcat tgatggttgt tggctatgcg gcactgggcg ccgatgcagt gccgccggct
     1801 tggcgcgcca cagactcggg tttcgaac
//
LOCUS       DAOSLE010000257         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_56780_length_1794_cov_10.714779, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000257 DAOSLE010000000
VERSION     DAOSLE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56780_length_1794_cov_10.714779"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1609)
                     /locus_tag="PK576_10810"
     CDS             complement(266..1609)
                     /locus_tag="PK576_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE62116.1"
                     /translation="MTNALFYRPLLEDLRCWRDDPNRKPLIVRGARQVGKSCLVRLFA
                     GEYPRYAELNLEKPSHAALFRRGLTLSELIQAIMLECRVPAGSSPLLLFLDEIQEVPE
                     AVAMLRFFQEERPDLHVIAAGSLLETALEAAAISFPVGRVQYLYVRPLAFAEFLAVMG
                     EDAAREALFTVPVPAFAHAHLLNLFHSYALVGGMPEAVLCYRDGGRDLQAVNRVYADL
                     LSAFRDDILKYGRTATQRRLLLHALESAPLVAGSRVTFQGFGGSDYRSREMGEALRTL
                     ERARLLELVYPTVQGVPPLMPDRRKAPRLQFLDVGLVCHMAGLQRHLIGIRDLSDVYR
                     GRLIEQVVGQELKAIDARADLPLIFWVRDKAQSQAEVDFLLQTTAGVVPVEAKSGTAG
                     KLRSLHEYLSRYPAPLAVRVYGGMYEEHPVQVGEKTCRLVNVPYYATGLLPRYIA"
BASE COUNT          406 a          536 c          529 g          323 t
ORIGIN      
        1 cggtccaatg gaagccggtg tggtagggga cgccgggctc gagaacggaa gccggctgaa
       61 cgcccaatag ctgcgcgtac gcatccgcga gatggaaagg atttttttgc gcggtatgaa
      121 ggggctccac cagttcctca aatttcatat acgaaagata acaatatcgt ttcttttcgt
      181 aaacaaaatt tgaggtgacg cgcgcaatcg gttggtggca aggggtgtgc gaaggtcatg
      241 caccatgatc tagggtggtc tcggcttagg caatatagcg gggcagcaac cctgttgcat
      301 aatacggcac attgaccaga cggcaggtct tctcgccaac ctgaaccggg tgttcctcat
      361 acatcccgcc atagacacgt accgccagcg gcgcgggata gcgcgaaaga tactcatgca
      421 gcgaccggag cttgccggcc gtgccggact tggcctcgac cggcaccact cctgcggttg
      481 tttgcaacag gaaatccacc tcggcctggg attgcgcctt atcacgcacc cagaagatca
      541 gcggcagatc ggcacgggca tcgatggcct tgagttcctg gccgacgacc tgctcgatca
      601 ggcgtcccct gtatacatcc gacaggtcgc ggatgccgat caggtgccgc tgcaatccag
      661 ccatgtggca aacaagacca acgtcgagga actgcaaccg gggcgccttc cggcggtccg
      721 gcatcagcgg cggcaccccc tggactgtcg gatagacgag ttccagcaga cgggcacgct
      781 caagggtacg caaggcctcc cccatttcac gggagcggta gtccgagccg ccgaatcctt
      841 gaaacgtcac ccgtgatccc gccacaaggg gcgcgctttc cagggcgtgc agcagcagtc
      901 gccgttgcgt cgccgtgcgg ccgtacttta gaatgtcgtc gcggaaggcc gagaggagat
      961 cggcatagac ccggttaact gcctgcaggt cgcgcccgcc atcgcggtaa cacaggacgg
     1021 cctcgggcat gccgccgacg agcgcatagg agtggaacag gttcagcagg tgggcgtggg
     1081 caaacgcggg gaccggcacg gtgaacaacg cctccctggc ggcatcctct cccatgaccg
     1141 ccagaaactc ggcaaaggcc aggggcctga cgtacaaata ctgcacacgc cccaccggaa
     1201 aggagatcgc cgcggcctcg agtgccgtct caagtagcga cccggcggca atgacatgca
     1261 ggtccggacg ttcctcctgg aaaaagcgca acatcgccac cgcctcgggt acctcctgaa
     1321 tctcgtccag gaacaagagc agcggcgagg agcctgccgg cacccggcac tccagcataa
     1381 tcgcctggat cagttcagac agcgtcagcc cgcgccggaa cagggcggca tgcgagggct
     1441 tttccagatt cagctccgca tagcgcggat actccccggc aaagagccgc acgaggcaac
     1501 tcttgccgac ttggcgcgca ccgcggacaa tcaggggctt gcggttcgga tcgtcccgcc
     1561 aacaccgcaa atcctctaat aaaggccggt aaaaaagcgc atttgtcaca aagtaaatcc
     1621 ttttttgttt cttttgtgtc acaaaataag ggcatttatc cgaaatgtca agaggcagac
     1681 agtaatccta aaaagagttg agccttcacc cctctttggt ttcaaggttt cgtggtttga
     1741 tccaaatcgg gatcgggatc gctatcggga tcgattgttt aaccacggaa caca
//
LOCUS       DAOSLE010000258         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_56823_length_1793_cov_8.493671, whole genome shotgun sequence.
ACCESSION   DAOSLE010000258 DAOSLE010000000
VERSION     DAOSLE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56823_length_1793_cov_8.493671"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..894)
                     /locus_tag="PK576_10815"
     CDS             complement(64..894)
                     /locus_tag="PK576_10815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOE62117.1"
                     /translation="MSEWTDILDGLTDQITLLKELGLRTVELDPAVIRALGRRAAPAA
                     DATRPPPDSQPPTVPAPIAPEPFTAPRVAPIEETQALSPEARRAALDELAARIASCTA
                     CPLHQSRNRTVPGQGNFNSPDILFIGEAPGAEEDQQGVPFVGAAGQLLTRMIAAMGYT
                     RDQVFIANICKCRPPRNRPPTPDEAAICLIYLRQQIRLIRPKTIILLGGTAIKALLDT
                     QVGVNRIQGEWTQYEGIPVMPTFHPSYLLRFEPAKRDAWQALKRVLAHLGKPVPSSSR
                     "
     gene            complement(897..>1793)
                     /gene="galE"
                     /locus_tag="PK576_10820"
     CDS             complement(897..>1793)
                     /gene="galE"
                     /locus_tag="PK576_10820"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOE62118.1"
                     /translation="RVAEITGARPIFRKVDLLDREALDTVFAEDGPFDAVIHFAALKA
                     VGESVGIPLTYYRNNLSGTITLCEVMTAHGCRNMVFSSSATVYGDPASVPIREEFPTP
                     GATNPYGWTKLMMEQIFRDLHRADPAWNIILLRYFNPIGAHASGRIGEDPAGLPNNLL
                     PYVAQVAIGRLPSLSVFGNDYPTPDGTGVRDYIHVVDLAVGHLKALEFLHTGPGLEVF
                     NLGTGKGSSVLEVVRAFEQASGRAVPYRIMPRRPGDVAACWADPAKAERVLGWKAERD
                     LRVMCEDAWRWQSANPNGYAGG"
BASE COUNT          323 a          547 c          605 g          318 t
ORIGIN      
        1 gggtcgacat aaggtctgac gggagaagcg ggcgtcccta ccccgcgtgg ccgggcggtt
       61 ttctcaacgc gatgaggagg ggactggctt gccgagatgc gcgagcactc gtttgagcgc
      121 ttgccaagcg tcgcgtttag ccggctcgaa gcgcagcagg taggaagggt ggaaggtcgg
      181 catcacggga atgccctcat actgggtcca ctcgccttga atgcggttga cgcccacttg
      241 ggtgtccagc agcgctttga tggctgtgcc gcccagcaga ataatggttt tcgggcggat
      301 gaggcggatc tgttgccgca gatagatcag gcagatcgcg gcttcgtccg gtgtcggtgg
      361 acggttccgg ggcggacggc atttgcagat gttggcgatg aacacttggt cgcgggtata
      421 gcccatcgcc gcgatcatgc gcgtcagcaa ctgacccgcc gcgccgacga acggcactcc
      481 ctgctgatcc tcctccgcgc cgggcgcctc gccgatgaac agaatgtcgg ggctgttgaa
      541 atttccctga cccggaaccg tccggttgcg gctctggtgc agcgggcaag cggtgcaaga
      601 ggcgatacgg gccgccagtt cgtcgagtgc ggcgcgccgg gcctcaggag agagggcctg
      661 cgtctcctca atcggagcga cgcggggtgc cgtgaaaggc tcgggcgcaa tgggggccgg
      721 gaccgttggc ggctggctgt ccggtggggg gcgtgtcgcg tcggccgccg gggcggcgcg
      781 ccgccccaga gcccgaatca cggcgggatc aagttccacg gtacgcagcc ccagttcttt
      841 gaggagcgtg atctggtccg tgagaccgtc caggatgtcg gtccattccg acacgattaa
      901 cctcccgcgt atccgttggg gttggctgat tgccagcgcc aggcgtcttc gcacatgaca
      961 cgcagatcac gctcggcttt ccagcccagc acgcgctctg ccttggcggg atcggcccaa
     1021 caggcggcca cgtcaccggg gcggcgcggc atgatccggt agggtacggc acggcccgaa
     1081 gcctgttcga aagcccgcac cacctccagc acactgcttc ccttgccggt ccccaagttg
     1141 aagacctcca gccccggtcc ggtatggaga aattccagtg ccttcaggtg gccgaccgcc
     1201 agatccacca cgtgaatgta gtcccgcacg cccgttccat cgggcgtcgg gtagtcattc
     1261 ccaaacaccg atagagaagg aaggcggcct atggccacct gggccacata aggcagcagg
     1321 ttgttgggga ggccggcggg atcctcgcca atccggccgc tcgcgtgcgc gccgatcgga
     1381 ttgaaataac gcaacagaat gatgttccac gccgggtcgg cgcggtgcag gtcacggaaa
     1441 atctgctcca tcatcagttt ggtccagccg tacgggttgg tggcgccggg cgtgggaaac
     1501 tcttcgcgga tcggcacaga ggcaggatca ccgtagaccg tcgcggatga gctgaaaacc
     1561 atgttacggc aaccgtgtgc ggtcatcacc tcgcaaagcg tgatcgtgcc cgaaagatta
     1621 ttgcggtagt aggtgagagg gattcccaca gattcgccga ccgccttgag ggccgcgaaa
     1681 tggatcaccg catcaaacgg tccgtcttcc gcaaagaccg tgtcgagtgc ctcccggtcc
     1741 agcaggtcaa ccttgcggaa aatcggccgc gcgccagtga tctccgcaac gcg
//
LOCUS       DAOSLE010000259         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_58083_length_1765_cov_12.680702, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000259 DAOSLE010000000
VERSION     DAOSLE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58083_length_1765_cov_12.680702"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..814
                     /locus_tag="PK576_10825"
     CDS             167..814
                     /locus_tag="PK576_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005022032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOE62119.1"
                     /translation="MEQIIQWVVNTVGQWGYAGIVIMMFLESSCFPFPSEVVMIPAGY
                     LSSPNYIHSEGYVAGHEMNIWVAIAMGIIGSWLGAMFNYYLALLLGRPLLLKWGKYFF
                     ISEENFEKAERFFKRHGEIGTFTARLIPVVRQYVSLPAGLVRMNMARFLFYTGLGAGL
                     WVSILAWIGYIAGQSEALWRPYLKRATVAALVFCVVVVAVYVLMRRARGKKSDAV"
     gene            820..1395
                     /locus_tag="PK576_10830"
     CDS             820..1395
                     /locus_tag="PK576_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE62120.1"
                     /translation="MGVCVREREQEATRRDALAWLLRAGAGAVVASFAAKAAQRPTAD
                     RTVWQIDPRACIQCGRCETECVLHPSAVKCVHGYAMCGYCKLCFGYFKSDAARLDEAA
                     ENQICPTGAISRVFIEDPYFEYHIDESLCTGCGLCVKGCTHFGNGSLYLQIRHDRCTN
                     CNTCRIAEKCPAQAISRVPASRPYLLKESLC"
BASE COUNT          295 a          492 c          569 g          409 t
ORIGIN      
        1 tttgcttggg gtgcaaaagg tctcgggttc aaatcccgac gccccgacca tttaaagagc
       61 gtcaggcctc gaaaacggat cgttttcggg gtttttgcgt ttaggccgcc gacctgttgc
      121 gcggcggtct atgcgcgggg gcggcgaacc cccaatcgga ggtggcatgg agcagatcat
      181 tcagtgggta gtgaacacgg tggggcagtg gggctatgcg ggcatcgtga tcatgatgtt
      241 tcttgagagt tcatgcttcc cgttccccag tgaggttgtc atgatcccgg cgggctacct
      301 ttcgtcaccc aactacatac actccgaggg ttatgtggcg ggccatgaga tgaatatctg
      361 ggtggccatt gcgatgggga tcatcggcag ttggctggga gcgatgttca actactatct
      421 cgcgttgctg ctggggcgcc ccctcctgtt gaagtggggc aaatactttt tcatttccga
      481 ggagaatttt gagaaagccg agcgtttttt caaacggcac ggcgagatcg gtaccttcac
      541 cgcgcgcctg attccagttg tgcggcagta tgtgtcgctg ccggcgggtc tcgtgcggat
      601 gaacatggcg cgctttctct tctatactgg actcggtgcc gggctctggg tttcaatctt
      661 ggcctggatc ggttacatcg ctggtcagag tgaagcgctg tggcggccgt acctcaagag
      721 ggcgactgtc gcggccttgg tgttctgcgt ggtcgtcgtg gcggtgtatg ttcttatgcg
      781 ccgcgcgcgt ggcaaaaaaa gcgatgcggt gtgagaccga tgggggtgtg cgtgcgcgaa
      841 cgggaacagg aggcaacgcg tcgggatgcg ctggcttggt tgttacgggc cggcgcgggt
      901 gctgtggtgg cgtcgttcgc ggccaaagct gcgcagcgac caactgcgga ccgcacggta
      961 tggcagattg acccgcgcgc atgcatccag tgcggccgtt gcgaaaccga gtgcgtgctg
     1021 cacccatcgg cggttaaatg tgtgcacggt tacgcgatgt gcggttactg caagctctgc
     1081 ttcggttatt tcaagtccga tgccgcgcgt ctcgacgagg ccgctgaaaa ccagatctgt
     1141 ccgaccggcg cgatctcccg cgtctttatc gaagatccct acttcgagta tcatatcgac
     1201 gagtcgctct gcacgggatg cgggctttgc gtcaagggct gcacccactt cggcaacggg
     1261 tcgctctatc tccagatccg gcatgaccgt tgtaccaact gtaacacctg ccgcatcgca
     1321 gagaagtgcc ctgcgcaggc gatttcccgc gtgccggcct cccgtcctta cctgctgaaa
     1381 gagagccttt gctaacccgg ccacacatag tcttacgttt catatggcgc ctacaaatgc
     1441 gcgacgaccg ggctcgcgga gcgccgggag gggcaagcgt ctgcttgcct gcggacgagc
     1501 agacgctcgt ccctcccatc ctgcgggcaa aacaaaagat ctgtgcaaat cttgtgtggg
     1561 tccctgcggc caccacggtc gcggcatcac gttttccgca gccccaattt gattcgggac
     1621 acaccgtgcc gcagacggtg catccgccct ttgaggcatt ggtccccgct tgggccgatg
     1681 tgctgatctt ggcgcttgcg ctggctgtga cggtgtggct ggcgcatcgg gtgcgttcac
     1741 gccgttggct gctggtgttc tctct
//
LOCUS       DAOSLE010000260         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_62369_length_1681_cov_17.637761, whole genome shotgun
            sequence.
ACCESSION   DAOSLE010000260 DAOSLE010000000
VERSION     DAOSLE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62369_length_1681_cov_17.637761"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PK576_10835"
     CDS             <1..1422
                     /locus_tag="PK576_10835"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOE62121.1"
                     /translation="GFVPPPPRQEIPPPPANMAGGETFIPYPPPPATPASRSEKKNPP
                     RPPTMFTKLTSQYGEQDWNARPNDLNNLLKALKDMAEVDYDFEAKSFAEIDADPDRNP
                     ILYRSGHYHWSLDDAEKKKLREYLLRGGTIILNAGMGSKPFYDSAIKNLNEVFPEAPV
                     RRLSPDHPIFHAYYSLDKVNYRPGVRALYNGNEPWLDGVTIDCRTVAIISRFGMEVGW
                     DPLEDDNIIAYGQESAQQLGMNILSYASAMRNWTLENMKKTRFEDAKQVSTAGSMNIA
                     QVIYDGEWKTRHVGLSVLLSQYNVKTGVPVKFSTKEIRLTDPKLFDSPVLYMTGHESF
                     TLKPDEITALRNYLTHGGLLIAEACCGRKAFDVSFRENMARVLPGTSFNALRTGHPLF
                     RLPNDISSMKVTPALAKSYNNAPAIAPQVLSLEVDGNPAVIYSPIGMAGAWELSPNKY
                     CNSYLEDDALKLGVNILMYSSTR"
     gene            1431..>1681
                     /locus_tag="PK576_10840"
     CDS             1431..>1681
                     /locus_tag="PK576_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62122.1"
                     /translation="MPVLHMFSKRRRAAPFFGAAIAVMLCVCARAQVATPQEEAFLKA
                     WGAHVRTPNDHKAVIEVCQSVMDKSSTLGEFLPVVKTLAA"
BASE COUNT          359 a          539 c          464 g          319 t
ORIGIN      
        1 gggtttgtcc cgccgccgcc ccgtcaggaa atcccgccgc cacccgcgaa catggcgggc
       61 ggcgagacct tcatccccta tccgccgccg ccggccaccc cggcgtcccg ttcggaaaag
      121 aaaaatccgc ccagacctcc gacgatgttc accaagctga cttcgcagta cggcgaacag
      181 gattggaatg cgcgcccgaa cgacctgaat aatctgctga aggcgctcaa agacatggcc
      241 gaggtcgact atgacttcga ggccaagtcg ttcgcggaga ttgatgcaga ccccgaccgg
      301 aacccgatcc tctaccgctc gggccactat cactggagcc ttgacgacgc ggagaagaaa
      361 aagctgcgtg agtatctgct gcgcggtgga acgatcattc tgaacgccgg catgggttca
      421 aagccctttt atgactcggc gatcaagaat ctcaatgagg ttttcccgga agcgccggta
      481 aggcggctca gccccgacca tccgatcttc cacgcctact acagccttga taaggtcaac
      541 taccggccgg gcgttcgtgc gctgtataat ggcaacgagc cttggcttga tggcgtcacg
      601 atcgactgcc gcaccgtggc gatcatctcg cgtttcggca tggaggtcgg ctgggacccg
      661 ctggaggacg acaatatcat cgcctacgga caagagtccg cccagcagct cggcatgaac
      721 atcctgagct atgccagcgc catgcgcaac tggacattgg agaacatgaa gaagacccgt
      781 ttcgaagacg caaagcaggt ctcgaccgca ggctcgatga acattgcgca ggtcatctat
      841 gacggcgaat ggaaaacgcg ccatgtaggc ctctccgtat tgctctcaca gtacaatgtc
      901 aaaaccggcg tcccggtgaa gttctcgacc aaggagattc gcctgacaga ccccaagctg
      961 ttcgattccc ccgtgctcta catgacgggt cacgaatcgt tcactttgaa gcccgatgaa
     1021 atcaccgcac tgcgcaacta tctgacacac ggcggcctgt tgatcgccga ggcctgttgc
     1081 ggccgcaaag cgtttgacgt ctccttccgt gagaacatgg cgcgcgtact gcccggcacc
     1141 tcattcaatg ccctgcgtac cggtcacccg cttttccgtc tgccgaacga catctccagc
     1201 atgaaagtga caccggccct cgccaagtcc tacaacaacg ctccggcgat cgcgccgcag
     1261 gtgctctcct tggaagtcga tggcaacccc gccgtgatct atagcccgat cggcatggcc
     1321 ggcgcctggg aactctcgcc caacaagtat tgcaacagct acctcgagga tgatgcgctc
     1381 aagctgggcg tgaacatcct gatgtattcg agcacgcgtt aatgccgttc atgcctgtac
     1441 tccacatgtt ctccaagcgc cgtcgggccg cgcccttttt cggggccgct atcgccgtga
     1501 tgctctgcgt atgcgcccgc gctcaagtgg ccacgccgca ggaggaggcc tttctcaagg
     1561 cgtggggtgc acatgtccgt acgccgaacg accacaaagc cgtgatcgag gtctgccaga
     1621 gcgtaatgga caaaagttcc acgctgggcg agttcctccc tgtcgtcaaa acgctggcgg
     1681 c
//
LOCUS       DAOSLE010000261         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_63113_length_1668_cov_6.429634, whole genome shotgun sequence.
ACCESSION   DAOSLE010000261 DAOSLE010000000
VERSION     DAOSLE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63113_length_1668_cov_6.429634"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /locus_tag="PK576_10845"
     CDS             <1..>1668
                     /locus_tag="PK576_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE62123.1"
                     /translation="RIGIKADGRVYAMFQNAGIHDQPTGVAVVSGRMLTANEWVHIAA
                     RMDGQEGTLKLYLNDETPISEGTRLIPANGVVNTTVDPGSGQYPVPHYLFSRPATLVL
                     GAANLNTLLNVTFPPWSEYAHFYQGYLDEVRIWDGARTDAEILNDYKRRYVKEDLLAN
                     RRAVRESEFAGGSRVPGVSPQLPPELLYHYTFDNLFGADNAESVAKVPRGFNYWETTI
                     NRPEGYQVGWWSSLPTASTVYDDYGYVPWIENGVEHLPLFGGIVAEGTNMVVRDVNMV
                     RNSFYWTHYGAGVSNTPPSVHGLERYAFPNSNDPYGYWYKTSLDILLLSGDAGHKSAA
                     DLLPLGGAFAKQAVVMWDNASPASVWADTGTDTDADGLPDWWEEYVATLGAEYENLGW
                     YDLYPDGSGMTVGEAYMRDVAKGYTASNNPAAEGWDPDALVRQTSDVDGDGLPDWWEN
                     LYNLNPSSALKQDGAAGDPDRDGLSNYAEYLISEVYGLRLSRPDRFKTSVTQTVSDYF
                     VREGKLYYGEMFADHDFMEDAWEDGYSAQFVNRYVYDAHLDPDQDGW"
BASE COUNT          320 a          511 c          543 g          294 t
ORIGIN      
        1 tccgcatcgg catcaaggcc gacggccggg tgtacgccat gttccagaat gcgggaatcc
       61 acgaccagcc gaccggtgtg gctgtggtca gcgggcgcat gctgaccgcc aacgagtggg
      121 tccatatcgc ggctcgcatg gacggccagg agggcacgct caagctctac ctgaatgacg
      181 agacgccgat ttccgaaggc acgcgtctca tccccgctaa cggcgtggtc aacaccacgg
      241 tggatccggg tagcgggcag tatccggtcc cgcattacct cttctcccgt cccgccacgc
      301 tggtgctggg cgccgcgaat ctcaacaccc tcctcaatgt caccttcccg ccgtggtccg
      361 agtacgccca tttctatcag gggtatctgg acgaggtgcg gatctgggac ggcgcgcgca
      421 cggacgcgga aatcctgaac gactacaagc ggcgctacgt caaggaagat ctgctggcca
      481 accgccgtgc ggtgcgcgag agcgagttcg cgggcggatc gagggtgccg ggcgtctcgc
      541 cgcaactgcc gcccgagttg ctctaccact acacctttga caaccttttc ggcgcggaca
      601 acgccgaatc ggtggccaag gtgccgcgcg ggttcaacta ttgggaaacc accatcaacc
      661 ggcctgaggg gtatcaggta ggctggtgga gcagtctgcc caccgccagc acggtctacg
      721 acgattatgg ttacgtcccc tggatcgaaa acggcgtgga gcatctgccg ctcttcggag
      781 gcatcgtcgc cgaagggacc aacatggtcg tgcgcgatgt gaacatggtg cggaactcct
      841 tctactggac ccactacggc gcgggcgtga gcaacacgcc gccttcggtg cacgggctgg
      901 agaggtacgc gtttccgaac agcaacgatc cgtacggcta ttggtacaag acctcgcttg
      961 acatcctcct gttatcggga gacgcgggcc acaagagcgc ggcggatctg ctcccgctcg
     1021 gcggcgcgtt cgccaaacag gcggtggtga tgtgggacaa cgcctcgccc gccagcgtgt
     1081 gggccgacac cggaaccgac acggacgccg acgggctgcc ggactggtgg gaggaatatg
     1141 tcgcgaccct cggtgcggaa tatgagaatc tgggctggta cgacctctat cccgacggca
     1201 gcggcatgac cgtgggcgaa gcgtacatgc gcgatgtggc caaggggtac accgcgtcga
     1261 acaacccggc tgcggagggt tgggatcccg acgcattggt caggcagacc tccgatgtcg
     1321 atggtgacgg cctgccggac tggtgggaga atctgtacaa cctcaacccg tcctctgcct
     1381 tgaaacagga cggtgctgcg ggtgatccgg accgcgacgg cttgtcgaac tacgccgagt
     1441 acctgatctc ggaggtgtac ggattgcgcc tgtcgcgccc cgaccgcttc aaaaccagcg
     1501 tgacgcagac ggtctcggat tatttcgtgc gcgaaggcaa actctactac ggcgagatgt
     1561 ttgcggacca tgacttcatg gaagacgcgt gggaggatgg ctacagcgcg cagttcgtga
     1621 accgctacgt ctatgacgcg catctcgatc cggatcagga cggctggt
//
LOCUS       DAOSLE010000262         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_64104_length_1649_cov_5.455458, whole genome shotgun sequence.
ACCESSION   DAOSLE010000262 DAOSLE010000000
VERSION     DAOSLE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64104_length_1649_cov_5.455458"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="PK576_10850"
     CDS             complement(<1..1098)
                     /locus_tag="PK576_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE62124.1"
                     /translation="MSETPVSNVRNFVILGHSGSGKTTLTDAVAFKLGLNDRMGLVTN
                     GSSVSDTTEEEKNRKITIFSSPFTASHKLGDAEYKMVFIDSPGFMDFCGQIQGAIRAA
                     DFALITVDAASGVQVGTRRAWKYCQDGGLASVAFVITGLDKDNTDFAKTVAAIRDTFG
                     VNCVPVTAPAGADAVINLLDASDLPTPLQEIRDSLAESAAETDEALMEKYFEQGSLDP
                     VDIRKGLVTGIQSGGTNPIYSVYPLKNVGVTEMLDSICRLLPAPGTRVYRDTEGNELA
                     PDPDAPAVAQVWRTAVDPFLGQLSYVRVISGTLTTGTSVQNTSTDTKESISSMIYVVG
                     KKQTPVTKAGPGDIVAIPKFKNTKTGNTLAAA"
     gene            complement(1311..1535)
                     /locus_tag="PK576_10855"
                     /pseudo
     CDS             complement(1311..1535)
                     /locus_tag="PK576_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
BASE COUNT          332 a          478 c          508 g          331 t
ORIGIN      
        1 ggcggcagcg agggtgttgc cggtcttggt gtttttgaat ttgggaatcg ccacgatatc
       61 gccggggccg gccttggtga caggcgtctg cttcttacca accacataga tcatggagga
      121 gatgctctcc ttggtgtcgg tggaagtgtt ctgcacgctc gtaccggtgg tgagcgtacc
      181 ggagatcacg cggacgtagc tgagctggcc gagaaacggg tcaaccgccg tacgccagac
      241 ctgcgccacg gcgggcgcgt ccgggtcggg agccagctcg ttgccttcgg tgtcgcgata
      301 aacgcgggta ccgggggcag gcagcagacg gcagatgctg tccagcatct ctgtgacacc
      361 gacattttta agcgggtaga ccgagtagat cgggttggtg ccgccggact ggataccggt
      421 gacgagcccc ttgcggatat cgacgggatc cagcgaaccc tgctcgaaat acttttccat
      481 gagggcctca tccgtttcag cggcggattc agccaggctg tcgcggatct cttgaagcgg
      541 cgtcggcagg tcgctggcgt caaggagatt gatcaccgca tcggcaccgg cgggcgcagt
      601 cacgggcacg cagttcacgc cgaacgtgtc gcggatcgcg gcaacggtct tggcgaaatc
      661 ggtattgtcc ttgtcgagcc cggtgatcac gaaagcgacc gaagccagac cgccatcctg
      721 gcagtatttc catgcgcggc gggtgccgac ctgcaccccg gaggcggcgt caaccgtgat
      781 cagagcgaaa tcggcggcac gtatggcgcc ttggatttgg ccgcagaaat ccatgaatcc
      841 gggcgaatcg atgaacacca tcttgtactc ggcgtcaccc agtttatgac tcgcggtgaa
      901 cggactcgaa aaaatcgtga tcttgcggtt tttctcctct tccgtagtat ccgaaacgct
      961 ggaaccgttg gtgaccagac ccatccggtc attcagcccc agcttgaaag ccacagcatc
     1021 ggtgagggtc gtctttccgc ttccgctatg cccgagaatc acaaaattcc ggacgtttga
     1081 cacaggtgtt tcactcatgt aaagcattcc attttttgcg ggcggatccg ctgctggtat
     1141 ccgccccatg ttatctccga atccgaaccc caccctcgtg gcattctgga cagaagtgtt
     1201 ataaaagagt atggcaaaga gacgaccctg aggcaatccc aaaagaccgt gcgccgcgca
     1261 ccggtcgaaa atcaggcacg ggaacaatca gaaaacggcg tgacacagcc tcatctccct
     1321 gccaatccct ggcgcccccc gtcggtcagg cggtattttt gcaggcgact gttgggcttg
     1381 tcggggatgg tcatctcaat cagaccgtca gccaaggccg gtttgaggta acgctcacgg
     1441 aaggatttgc ggtcagaaag ccccagggcg gattgcagcg cctcgcgccc catctcgcct
     1501 tggagtgccg ccagcagctc gccgacttgg ggggacttga gggggggtta cggcgatagg
     1561 tatatcgcgg gacggagcga tgacccgttt gccttccaga atcgccgagc aacgcgccaa
     1621 ttctccaagc ggcgcaacgc cccggatta
//
LOCUS       DAOSLE010000263         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Kiritimatiellia bacterium isolate AMR_MDS_1631
            NODE_66981_length_1598_cov_9.418017, whole genome shotgun sequence.
ACCESSION   DAOSLE010000263 DAOSLE010000000
VERSION     DAOSLE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863160
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kiritimatiellia bacterium (wastewater metagenome)
  ORGANISM  Kiritimatiellia bacterium
            Bacteria; Kiritimatiellota; Kiritimatiellia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:38:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,172
            CDSs (total)                      :: 2,139
            Genes (coding)                    :: 2,122
            CDSs (with protein)               :: 2,122
            Genes (RNA)                       :: 33
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 15 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Kiritimatiellia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66981_length_1598_cov_9.418017"
                     /isolate="AMR_MDS_1631"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2212473"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1443)
                     /locus_tag="PK576_10860"
     CDS             complement(169..1443)
                     /locus_tag="PK576_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE62125.1"
                     /translation="MKAKRPKIKTYEHHGIKIRELRPELPEGGGGYYQVDLMRRGRRE
                     RSGFDTLQKAKTYCQQIGGKIEDEGAAVLAMSPTERHDAVQAQEELRGKATLLDAARF
                     WMKHNGGEDGVTVEEAGRRWLAELRRQGCRPTTIRARTFMVDSIVRFMGKKPVAAVTR
                     GDLEKWMEGKTGQTWDTYRRTARALLQYATEEKLVDYNVAAALRPVRADEKLPVPFTV
                     AAVSDILRAAEKYAPEVVPVLAVQFFCGLRPTEALGLRWEQVDFKSKTVRVLPETSKV
                     RRTRIVSPISAAALAWLAPYRKTRGPIGITTPSQQSYFMHRKPCGPDYVQKGVPIAER
                     KPDTRPKGLLSAAGVEWLQDGPRRTFASAHYALHGDAAKLASILGHTGGHDVLFRHYR
                     GLMTKAQARRFFAIRPASSNKVVVGNFKLAAG"
BASE COUNT          270 a          520 c          464 g          344 t
ORIGIN      
        1 tttcatggtg acaatccttt caactgtcac cagtttccca cgggtacgcg gaaacggtca
       61 aaaaacgagc gcacgatggc gcagagaacc cgtatttagg gcatattccg catattccgc
      121 ttgcggcggc ttatgctcgc aattcccccg atcagcagca gatggacgtc agccggcggc
      181 cagcttgaag ttgccgacga ccaccttgtt cgacgatgcc ggacggatgg cgaagaaccg
      241 tcgcgcctgg gccttggtca tcagaccccg gtaatgccgg aacagaacat cgtggccccc
      301 ggtatggccc aagatgctgg ccagcttcgc cgcgtcgccg tgcagggcgt aatgggccga
      361 cgcaaaggtg cggcggggtc cgtcctgtag ccattcgacg ccagccgccg acagtagccc
      421 tttggggcgc gtgtcaggct tgcgctcggc aatcgggacg cctttttgca cgtaatcggg
      481 tccgcatggt ttccggtgca tgaaatagga ctgttggctc ggcgtcgtga tcccgatggg
      541 gccgcgtgtt ttccgatagg gtgccagcca tgccagcgcc gccgccgata tgggggaaac
      601 aatccttgtt ctcctgacct tgcttgtctc tggcaggacc cggacggtct tgctcttgaa
      661 atctacctgt tcccatcgca gccccagggc ctcggtcggg cgaagaccac aaaagaattg
      721 aacggccagc acggggacaa cctccggggc gtacttttcc gcagcccgca gaatatccga
      781 gaccgccgcc acggtgaagg gaacgggcag cttttcatcc gcccggacgg ggcgcagcgc
      841 cgccgcgacg ttatagtcca ccagcttttc ctcggtggcg tattgaagca atgcgcgcgc
      901 ggttcgcctg tacgtgtccc aggtctggcc cgtcttgcct tccatccact tttccaggtc
      961 cccccgcgtg actgccgcca cgggtttctt gcccatgaag cgcacaatgg aatccaccat
     1021 gaaggtccgc gcccggatcg tggtgggcct gcaaccctgc cgccgcagtt cggccagcca
     1081 gcgccgcccc gcttcctcta ccgtgactcc atcctcgccg ccgttgtgct tcatccagaa
     1141 ccgcgccgcg tctaggaggg tcgccttgcc ccgcagctcc tcctgtgcct gcaccgcgtc
     1201 gtgccgctcg gtaggggaca tggccaggac cgccgcgcct tcatcttcga tcttcccgcc
     1261 gatctgctgg cagtaggtct ttgccttttg cagcgtatcg aagccggacc gctcccgccg
     1321 tccccggcgc atcaggtcaa cctggtagta tccgcccccg ccctcgggca gctcggggcg
     1381 cagctcccgg attttgattc cgtggtgttc gtaggtcttg atcttgggtc tcttggcttt
     1441 catcgtgcgc cctttcgcgg atggttgttt atccatgcac aagatagcac tttgtcaaag
     1501 cgtgtcaaga ggatgaaaaa cactaggaaa acggctatat tccgccttac ttgccaaggt
     1561 ggttgtcgtg ggttcaaatc ccatcgctcg ctccattt
//
